LOCUS       DAOPMW010000001        41813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_774_length_41813_cov_8.701470, whole genome shotgun sequence.
ACCESSION   DAOPMW010000001 DAOPMW010000000
VERSION     DAOPMW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 41813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41813
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_41813_cov_8.701470"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLU11_00005"
     CDS             <1..398
                     /locus_tag="PLU11_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57511.1"
                     /translation="TTFSTGFMFFSGASLTIDADSKEVDLLFSLEGANRCFDNNSTAV
                     VHFEGTKVKMNLRNAGTMNCEGLFHFIYKNSVNTTTQLQKLTTQRVSQIVFTDANKKE
                     TTVLLTGKDQELLISLGTCLVTEAKTLIR"
     gene            581..1009
                     /locus_tag="PLU11_00010"
     CDS             581..1009
                     /locus_tag="PLU11_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57512.1"
                     /translation="MNGQLTATGNTIVPVSLNQLIKSLVENTQPLAIQHHTKVVNEVD
                     GRLLLGEGASQLSSVLEELLDAVILNSREGDIHITADRFKDMVLLHIEERNNYNGYAL
                     SFSVGSLEPDAARIGGHISIEGERKKEAIIQFSFPGQFAA"
     gene            complement(1061..1633)
                     /locus_tag="PLU11_00015"
     CDS             complement(1061..1633)
                     /locus_tag="PLU11_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57513.1"
                     /translation="MKKLIALTALLFSLTPFTPLLAQAPAGFTAGYIVTADGSRLEGF
                     IKENFKGKASFVFQQTGGKKVTYGGNAVNEVNVGGVAYISYANDFFKVVTAGTRASLL
                     QKVSDATGKVIYNGNEAAGISSGTEGRINDLFIKTNGQSGLTLVSKDNLNTVAVNLFG
                     DCTSLMDSAKAGQLQFTDLEKIIARYNECK"
     gene            complement(1705..2277)
                     /locus_tag="PLU11_00020"
     CDS             complement(1705..2277)
                     /locus_tag="PLU11_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN57514.1"
                     /translation="MAARPTDTKESILRLGMEFIQTYGYHSFSYADIAKELKIKNAAV
                     HYHFPGKEDLLAEIVEQYIQRYHELARSLEKPGISSRQKIQAFINRYTALCEHGKICL
                     IGSVGSDYNTLPEKAKVKLTELVKLVLGLVEKVLREGKKKGELTFSETARVKSILIMT
                     NLAAGVQLARITGQQDYTAICNSLMKQLTG"
     gene            complement(2374..3699)
                     /gene="ahcY"
                     /locus_tag="PLU11_00025"
     CDS             complement(2374..3699)
                     /gene="ahcY"
                     /locus_tag="PLU11_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPN57515.1"
                     /translation="MSTVSKSIDFSLPYKVADISLAEWGRKEIRLAEAEMPGLMAIRS
                     EYGPSQPLKGARVAGCLHMTIQTAVLIETLTALGAEVKWSSCNIFSTQDHAAAAIAAA
                     GIGVFAWKGQTQEEADWCIEQTLFFGAADRPLNMILDDGGDLTNMVLDTYPELVQHVK
                     GISEETTTGVHRLYERVAKGTLPMPAINVNDSVTKSKFDNKYGCKESLVDSIRRATDV
                     MLAGKVAVVGGYGDVGKGSAASLAGAGCRVIVTEIDPICALQAAMDGFEVKKMIDAVK
                     EADIVVTASGCRDLITGAHFKLMKDKAIVCNIGHFDIEIDIAWLNTNYGHTKDTVKPQ
                     VDIYNVDGHDVIILAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIELWNNHKNYENKV
                     YVLPKHLDEKVARLHLAKIGVVLDELTDAQAEYLGITKAGPFKSELYRY"
     gene            complement(3954..6353)
                     /locus_tag="PLU11_00030"
     CDS             complement(3954..6353)
                     /locus_tag="PLU11_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPN57516.1"
                     /translation="MKKAVRIFWRIFFGGLAAFILVIVLANFGVFGAMPSINELQNPS
                     ILQASEVYAEDGTLMGKYYTERGNRSNVKYRDISPYVIDALVATEDERFYSHSGIDFR
                     STMRAVFTLGSEGGGSTITQQLALNLFNERATNKALRVIQKLKEWIIAVKLERNFTKE
                     EIITLYLNAVPFGNNVYGIRNASRTFFQKEPDRLTVEEAALLVGMLKGNSIYNPQRNP
                     KLALDRRNTVLSQMEKNGKITAAEAKKAKALHIKLNYRKLDENTGYAPYFREVLKDEI
                     KEALKDVEKPDGGKYSIYNDGLKIYTTINVQMQQYAEEGMAQHMAMLQKGINQRTDMK
                     NGTVWKGREKLLEKAIKESDRWKNLEEDGLSDKEIKASFNVKVPMKIFSWNNKREKDT
                     VMTPLDSIKYHLQMEQAGFIAMDPVTGEIRAWVGGIHFKTYKNDHVNLKTKRQVGSTI
                     KPLLYTQAMEERGFTPTTECENVAQFFQGSGWVPSGKQCGGGSMTMADALAFSKNCAT
                     AYIMKQVQPKQFSEFLARINIPTPVKPYPSIALGTCDLSMYEMLWSYTMFAGRGFSTK
                     PYAISRIEDRNGNVIKRFDFSVNRKEVISEATAYTMARMMQGTVDKGTAKGMRTRVGA
                     AEMGGKTGTTDDNADAWFIGYTPQLLAGSWVGFEYTFMHNQGDGSRIARPIAEYFFQK
                     VLADKKLGIEKDAKFIKPADLEEGPNNADIIISEQDPVPGAEGVDQGTGTEEDYQLNN
                     NEYISPESTPVKEDNMPAKRDSIVNTPVKKETETKPIGSAQEDPKKKKGLLKRLFDRK
                     N"
     gene            complement(6506..9463)
                     /locus_tag="PLU11_00035"
     CDS             complement(6506..9463)
                     /locus_tag="PLU11_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57517.1"
                     /translation="MRAKRHIIFFLFSLILLQAGLPAIGQLGFDLNIKKPKEYEERVL
                     RSEKTDKKFTVPRRFVQNTVTHYNYTFNANNKLNDVLARAKQAFKDDYSKLLPFYNYS
                     LDVTAADSLQLDSITLKASSGIALHDLRNDWVDNLYLLWGASYYLQKDFDSAYQMFQF
                     INYAFAPKEKDGYYKTIGSARDGNSALSISTREKRGLAKKMFTEPPSRNDAFIWQIRN
                     FLAQDQFAEASSLIVTLRDDPAFPSRLMNDLHEVQAYSFYKQNNWDSAAFHLVEALSN
                     AGNQQERARWEYLAGQLYEMAGNFKEARKNYDRSISRTTDLVMEIYARLATIRTNKDD
                     GEADIQKNVAELVKMARRDKYADYQDIIYYMAAQMQLEGNKVEEALQLLLLSTQAPNN
                     NPGQKNKAFLQMGDLSFLQKAYKNAYAYYDSIKLDDPSITNPDAISSRKTALREIVVN
                     IEIRERQDSLQRIAALPEDERRDYVKKLVKYLRKQQGLKDEQPILTTGIQTTGNQPPV
                     TLFPTGDTKGEWYFYNTNARSRGQNEFRAKWGNRPNTDNWRRGAAILNNRMNASPGGS
                     NPQDPGSNSGGDMSNGISFDDLYARLPLTEAQLAQSNDSIQKALFNLGKTYIQGIEDC
                     SAGTATLEELRATYPDFMPMDEVLFNLFYCYNKNGETGKAAEIKKLMEGKFAASNLTT
                     IVLTGKNPADETGNSAAKKTYEQIYDLFIEGDFAAAIAMKRTADSLYGNNYWTPQLLY
                     IEAVYYIKQRDDNEAKDILNSIISRFPKSPLNEKSIDLIDVLSRRAQIEEELTNLQIT
                     RYPDSGTVAPVTVIPAPPVTKTDTGSVKPPATVMPPTVKQPVQDTVKTQPVAPPPVVT
                     STPFVFKPADKYFVVLVLNKVDPVFINEAKNAFFRYNRETYYNKTYTADLYQLDEENR
                     LLLMSFFQNTDEAVAYVEKTRPKTASDIIPWLKGGKYYFTIISESNLQLLKAGKDLEA
                     YKRFLEQHLPGKF"
     gene            9597..9995
                     /locus_tag="PLU11_00040"
     CDS             9597..9995
                     /locus_tag="PLU11_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57518.1"
                     /translation="MAKLEAGSTLQRLRNRYRLVVMNDDTYEEVVTFKLSRLSVYVML
                     STIFVVLVGLTVALIVFTPLKYYIPGTSNDYKSVMELKRLQYRVDSVERQDAYKTQYI
                     LGLKKALGSTETAKLDTTAIVVPKPDISND"
     gene            10034..10432
                     /locus_tag="PLU11_00045"
     CDS             10034..10432
                     /locus_tag="PLU11_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPN57519.1"
                     /translation="MQQPVSNGNGGGATLISAGTVLKGDISSSSDLRIDGTVIGNIKS
                     GSKIIVGSSGVVEGDITGNQADITGKVNGNIRAKELLQLRGECLVSGNIYAGKLQVEP
                     SATFNGQCHMGANIVEMPNNESAVTVAAAK"
     gene            10625..11035
                     /locus_tag="PLU11_00050"
     CDS             10625..11035
                     /locus_tag="PLU11_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57520.1"
                     /translation="MNRSLLQPIRPLFLVFILVNALALTAKSLLEKYHFNQELLILGN
                     LLLFVVILVSYLITYRSLHSSNSQAFIRAMYGSFLIKFFILAIAAFTYIMIAKKEVNK
                     PGLAVCALLYIIYTAIEIRALMSTLKRMKQQSHG"
     gene            11028..11657
                     /locus_tag="PLU11_00055"
     CDS             11028..11657
                     /locus_tag="PLU11_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPN57521.1"
                     /translation="MAKKEVPVNHLEQFLPPGTGEAVLHYLHEYKVHLTIARERKSIL
                     GDYRHRTHQQNHRISVNGNLNPYAFLVTLLHEIAHLLTFEQYGNRVNAHGREWKSVFG
                     KLLHQFVQNQVFPPDIQQELTESLKNPAASSCAEDGLLRVLRRYDAKKDNHHLIEEFP
                     MGSLFRIKDGRVFRKGEKLRKRYKCTELPSGKVYLFSPVYEAVLVTVSS"
     gene            complement(11795..12289)
                     /locus_tag="PLU11_00060"
     CDS             complement(11795..12289)
                     /locus_tag="PLU11_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN57522.1"
                     /translation="MPPTIRPIQPADNAVIAKIIRDTLTEFGANHPGTVYYDPTTDTL
                     FELFQQEKSVYNIAEIDGKIVGGGGIFPTAGLPADTCELVKMYLLPEARGTGLGTQLI
                     NRCLEQAAANGFSKVYLETMNELKAALKVYERLGFSYLSGPMGNSGHFGCPLWMMKEL
                     EARS"
     gene            complement(12485..14368)
                     /locus_tag="PLU11_00065"
     CDS             complement(12485..14368)
                     /locus_tag="PLU11_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPN57523.1"
                     /translation="MRQLIMSLACLCILQVNAQTKKTVDSKIEKVTVFLQGAQTQRGA
                     KVSLTPGKYELVFQSISPSIDAQSIQVKADGAITVLSVIHQQNFMKEQQKQEEIRELE
                     TQKEALLEKIALQRNILNVFKQEEAMLLKNQQIGGANTGLKAADLKEAADFQRNRLTE
                     VYQKQMETDRVIKKQDAEMAKLNKQLAELNQKADISTSEIHVTVNAKETTNSQFTLTY
                     LVRKSGWYPTYDIRVKDISSPINLQYKANVSQQSGEDWKDVKLYLSTGNPNEDGTKPV
                     INPWFLKYYYPTASVNPLQGRVPGLSLNTPGMVSGRITDQQGNPVPGASILIKGTKTG
                     AAADANGNFSIRTGTGNPVLEISAVGMERAEYYANSGFMNITLQPSSNALSEVVVIGY
                     SGGSGYDDYQPGASPNYRKKKELTAINTTTSYQPTTTIYEIDDPYTVLNDGKTYTVDI
                     DGYELKAQFEYYAAPKFEPDAFLTARIADWQELNLLPGEANLFFEGTFLGKSTLDVMN
                     AGDTLNLSLGRDKGVVVKRTLLKEYSSRKFMGSNKTDTRQYEIVVRNNKQQPISITID
                     DQFPISTMKEIEIQDRKYDGAKLDDDTQKLTWQLTVDPKKENKLGFKYEVKYPRDKVL
                     QLD"
     gene            complement(14416..15510)
                     /locus_tag="PLU11_00070"
     CDS             complement(14416..15510)
                     /locus_tag="PLU11_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57524.1"
                     /translation="MRYLFVALILIHGLIHFMGFSKAFGLADMKQLSISISKPIGLLW
                     LLTALLFLISATLYLLKYGSWMYTCIIACLLSQVIIVLSWKDAKYGTIANGILLLIAL
                     LSLTTRLFEQSYKKEAAELVKKNSLNAIPLLTSADIGHLPVPVQRYLIYCGVLNQPKL
                     LNMQVMFEGQMREKGKDFFSFRSEQYNFFENPARLFFMKAAMYGITVPGYHRYVDASA
                     TMDIRLFGKIPVIHQSGPDMDTTETVTLFNDMCLLAPASLIDSRISWEPIDSLTVRAV
                     FINRGISISATLYFNKAGQLINFSSNDRMAIADHKRIPFSTPIHEYRPVRGFNLVSEA
                     DAVWDYPDGKFVYGKFRLTDIRYNINDREN"
     assembly_gap    15539..15638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15639..16744)
                     /gene="sucC"
                     /locus_tag="PLU11_00075"
     CDS             complement(<15639..16744)
                     /gene="sucC"
                     /locus_tag="PLU11_00075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPN57525.1"
                     /translation="MNLHEYQAKELLKKYNVPVQEGFACSTVHEAEEAYRQIKNQFGS
                     SFAVVKAQIHAGGRGKGAIKENGINGVKVGKNIEDIADFAKKILGGTLVTLQTGPAGK
                     VVNKILVAQDMYYDGISDRKEFYLSILLDRTKGQNVIMYSTEGGMNIEEVAHNTPEKI
                     FKEWVHPSGGLQGFQARKIAFNLGLSGDAFKNCVKFVTNLYNAYVGLDCSMLEINPLF
                     KAADNKIVAVDCKMNLDDNALVRHADLSSLRDVTEEDPTEVEAGQFNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFKIILKDPAVKAILINI
                     FGGIVRCDRVAQGVIDAYKNLGNIHIPIIVRLQGT"
     gene            16823..17635
                     /locus_tag="PLU11_00080"
     CDS             16823..17635
                     /locus_tag="PLU11_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57526.1"
                     /translation="MRLFPILAVFYLAGCHPGYRMQWPENRTGLTGSTFYEQAAAMQW
                     AARDSLVVQAALSGNLPPFLRKFVPVRVSLTDSLSGKMIKAVFYVAPDYLSIGSGDDW
                     ARVCITPYAAQRIADSLNCFLPTRKMVDDIYQAATLKLRPVPLFAYRDSTPSMWQHHL
                     IIEGQRKGKKGLIAGIKKDVVISGKISRDKKPDRVAIYGWHQPGGRVIQPLYTGHIYW
                     WVDYSQGIRLVYRRINVDGRWMDYTEVLKNPRLSRLLCDEKDCDFLRYPVHP"
     gene            17713..19773
                     /locus_tag="PLU11_00085"
     CDS             17713..19773
                     /locus_tag="PLU11_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57527.1"
                     /translation="MQRYFIFYQISFTLRSNYIYLSLFLLLFISQALLAQEPAMQLGN
                     RLKNLGSSFSGGSGNDSLKHRDNSEDSITISFRYMDSTRQYKLDSSVGDFTRRFPLPG
                     TSINLGNTGLATRSLLFSPELNAGFDPGFHAFDVYKWKLSEVRFFNTTRPYSELNYLL
                     GSRVEQMISLVHTQNIKPNWNFLFQYRLINSPGSFQNQNSNHNNYLLSSKYQSKNLRY
                     NNYFVLLSNKLQAYENGGIIDTTDILNDPDFKERYNINTRIGGKKVFSTNFFGNKINT
                     GNKYSETTLLFRQQYDFGKKDSLVTDSTIIPLFFPRLRFEHTLQFDRNSYTFQDNAGD
                     SLYYRAYYDTALRDANDTALFSDKWKLLNNDFSIYQFPDAKNLHQFIKLGLLVQTITG
                     TLSSGKLNFFNTAGHAEYRNKTKNQRWDIQANGRLFFTGYNSGDYEAHIRLQSLLSKK
                     AGSLELGFENTSRTPSFMFDSRSSFYLMKSVVNLKKENNTHLFASYFLPSFKMKLTGH
                     YYLVTNYTYLDNYFEVNQESSLFNLLQIAVEKTIRFGKHWNWHAELYFQQRVGEAPVN
                     VPALYTRNRIGYEGSLGFKNLDIAFGTEIRYRAAYKADHYSPVMGRFFYQDTTVIRNP
                     LPDIAAYVHFRIRPFTAFVRMENLNTARNFNGFGFTRNNLVAPFYALPGLQFRLGVFW
                     RFVN"
     gene            19843..20235
                     /locus_tag="PLU11_00090"
     CDS             19843..20235
                     /locus_tag="PLU11_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57528.1"
                     /translation="MKKLVLTISFLCYLTVTCGVVVNLHYCMDRLASVHFFGDESDQC
                     GQCGMLLHESNTCCHDEVKVFKLEQDQNKVSITDYTPVFTTELTIEPSLFLNAPFESE
                     LGLVNTFVNPPPLLSEQDTYLQINVFRI"
     gene            20329..20988
                     /locus_tag="PLU11_00095"
     CDS             20329..20988
                     /locus_tag="PLU11_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPN57529.1"
                     /translation="MKTIRLFSLTAVMVAITSLGFAQTKTESFQVSGNCGMCKTRIEK
                     AAKDAGASEAKWDAATQQLTITYTSSTTNAAKIQEKIAAVGHDNAGFKSTMEVYNKLP
                     GCCKYDKDAASGSMDCCKDGKCTNAGHCDKPCCKKDGDMKMDCCKDGKCDKEGHAGKD
                     CCKKEGAAKMDCCKDGKCSKEGHDGKDCCKKEGAAKMDCCKDGKCSKEGHDGKDCCKK
                     S"
     gene            21009..21554
                     /locus_tag="PLU11_00100"
     CDS             21009..21554
                     /locus_tag="PLU11_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPN57530.1"
                     /translation="MKKTGLLIIMALQVLLIQAQFSKANLQATGLTCALCSNAINKAL
                     KQLPFVESVKADIKNSSFSIVFRQGMDVKPDAIRDAVEGAGFFVGGLQLTGSFNNLST
                     GPDGIIRLGTNWFRFLNSSNKVLNGEVTFTLQDKSFLTAKAFKKISSTVKIPSLSTGK
                     AEADQVKAGVKKGERIYHVTI"
     gene            21624..22106
                     /locus_tag="PLU11_00105"
     CDS             21624..22106
                     /locus_tag="PLU11_00105"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN57531.1"
                     /translation="MKQIIVVFFLLCMSTLAQAQIKQGYKAPEVALPAANGDTIRLSS
                     LKGKVVLLDFWASWCGPCRISNKGLVKLYPKYKDKGFEILAISLDEDVDDWQKAIKKD
                     KVSWLQVIDKGGWESGTVNQWRIVAIPTSYLINKDGVLVAMDLEGKNLEKALNELLGD
                     "
     gene            22120..22959
                     /locus_tag="PLU11_00110"
     CDS             22120..22959
                     /locus_tag="PLU11_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57532.1"
                     /translation="MRYLIISGLLILLSVQGNAQELYVFSEPASNLPARSLSVKIKNH
                     FVTRDNIYGRFSNRLMPQFFFGVHKNLMLRAGISVANMHTPDTRYESFNVYAKYRLVS
                     NDDIHRHFRLAVYSEFSKTRIPFHYDEITLMGDKGGVEAGLVATQLWHKFALSGTIGH
                     TQVLHKSRKDNVIYIPERGYQSLNYSLSGGLLILPKEYSDYKQTNLNLYLEIIGQQLM
                     DKKAGFLDLAPAVQLIFGSTTKLNMGYRFQLNGSMQRMASNSWLISLERTFLNALKKK
                     HPK"
     gene            22965..23360
                     /locus_tag="PLU11_00115"
     CDS             22965..23360
                     /locus_tag="PLU11_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57533.1"
                     /translation="MRKTGLIILTFLYLAFTAGISVQRHYCMNRMAALRFTTQSEERC
                     GFCGMEKTERDNSCCHDEQAVYKITDDQQTGFPTIFIVNEFQVGSPEHYIPVSGKIIT
                     ASVPVPAYSHGPPGVSAFPLFIRFGVFRI"
     gene            23436..25982
                     /locus_tag="PLU11_00120"
     CDS             23436..25982
                     /locus_tag="PLU11_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57534.1"
                     /translation="MKIIILLVLFFLLFFSRKDIQAQTSSVITITLKVSGNCEMCKER
                     IEAAAKGKGVKEVSWDAETKLLTLTYDTLLTRIEKVHIRIADAGHDTELKKAKNDTYK
                     ALPECCLYRDHSESMTTDSGNSLISLVRGVVLEEDDRGNFRPLPRASISWLGTGAGTL
                     TDSTGVFKISVTQETDRLVISYTGYRPDTIAVQPATDLKIILASNQQLGEVTLNSKLR
                     STYISSLSTIRTQVMTEKELFKAACCNLSESFETNPSVDVSYNDAVTGSKQIQLLGLS
                     GNYTQLTVENLPGPRGLATALGLNSIPGPWVESIQLNKGVGSVVNGYESIAGQINIEL
                     KKPEKAEQLYANVYLNNMGKSDINLNLSRKFGKKWSAALLLHDDFLYNKVDFNKDGFR
                     DLPTGNLFSVLNRWKFENGKGFMAQFGVKLMTDEKTGGETDFDPAKDKLTTNHYGLGI
                     STRRYEAFSKTGYVFPGKKYKSIGLQLMAFSHEQDSYFGTQPYLAAQQSLYANLIYQS
                     IIGNTNHKFRTGLSWTADRYDETIRNITYKRTERVSGGFFEYTFTPNPKLGIVAGIRS
                     DHNNLYGWFLTPRLHLRYEPVKGTSIRISAGRGQRTANIFAENNSVLVSARTIVMPAL
                     NTSAYGLNPEVAWNKGLSVDQQFRLFNRSASLSMDFFRNDFRNQVVVDLEKAREVSFY
                     NLSGHSYSNSLQVELHTIPLRNLEWRLAYRYFDVHSTYSGVALQKPFTSKHRAFMNLA
                     YEIKDWKLDYTLNLNGPKRIPSTRDNPGFYQRDNYSPSFWIMNAQVSKTVGKKHPVDL
                     YLGGENLGNYFQRDVIISPDQPFGAYFDASLVWGPVSGRMIYAGLRYKIN"
     gene            26135..28417
                     /locus_tag="PLU11_00125"
     CDS             26135..28417
                     /locus_tag="PLU11_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57535.1"
                     /translation="MKQKPLFLLAIYCWFMLPLAAQTIRGEVRDALSGEPIPMASVHA
                     GDSLKTLTDKQGRFTINTSSNVHIHVSAMGYETAAFLNNQKDFLTITLMPQQKQIEGV
                     VITGTLKAVRRSESPVAVEVYTPQFLKKNPAPSLFESLQNVNGVRPQLNCSVCNTGDI
                     HINGLEGPYTMVAIDGMPVMSSLASVYGLFGIPTQLIDRIEIVKGPASGLYGAEAIGG
                     MINIITKSPDKASPVTAQLMTSSWKEHSFDAGLKFNLGKKISSLLGIHYFNYQNKVDH
                     NQDQFTDVALQHRVSLFNKFQLTRKHNRLASLAGRYFFENRWGGDTRWTKEFRGTDSF
                     YGESIYTSRWELFGQYQLPVQEKFLFSFSTTGHKQDSYYGQLPYLATQRTGFMQLTWD
                     KIAGENHSLLTGISARANFYDDNSTATKDTLSGRNKPDRSVLPGLFIQDEWKLHEKHL
                     LLLGLRTDYHPIHKSILTPRFAWKWSPGHQQVIRLNGGTGFRVVNLFTEEHAALTGAR
                     VVEIREALRPERSYNINLNYTRLLKGNKYSLQLDGSAWYSYFSNQIIPDYDTDPNKII
                     YKNLDGFSFSKGMTLNIDYSYGHRFKALLGMTLQSIKKTEKDASGNKLTQVPVLTEKW
                     SGTWSISYSIPAGGWSVDYTGNIYGPMRLPLLGVRDPRPAHSPVWSLQNLQVTKKLGR
                     AIECFAGSKNLLNWTPARRVPFLIARAHDPFDKLVEYEPDGSVRVSAENPFGLSFDPA
                     YVYAPNQGRRIFAGLRFTLR"
     gene            28461..28847
                     /locus_tag="PLU11_00130"
     CDS             28461..28847
                     /locus_tag="PLU11_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57536.1"
                     /translation="MKRFAGILLISVYMFSFAEFHNLLKIPVLIEHFHEHHQQNSNTS
                     FWSFIKLHYFDAYVIDSDYQEDRQLPFRDTDCGLVVSTSICEYAPVVMDIQPPAEPVR
                     EFHHFNDGHAPQFTSFDIFQPPRVLA"
     gene            28904..33274
                     /locus_tag="PLU11_00135"
     CDS             28904..33274
                     /locus_tag="PLU11_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPN57537.1"
                     /translation="MLNHIIRFSIRNKLIIGLLMLGLIGWGAISFSKLPIDAVPDITN
                     NQVQVITVSPSLAAPEVERLITFPIEQAMASVPGIEEIRSFSRFGLSLVTIVFDDNTN
                     VYWARQQISERLQQVRAEIPADAGNPFMGPVTTGLGEIFQYAVKPKKGFESRFTPAEL
                     RTIQDWIVRRQLLGTPGVADVSSFGGNLKQYEVSINTDRLKSMNVTVDDVFNALQLNN
                     QNTGGAYIEKGPTVLYIRSEGLAKTIEDIGKIFVRHTSSGIPVYIKDVADVKTGSAVR
                     YGAMCYNDEGEVAGAVVMMLKGSNSSVVIKDVKEKITQIEKTLPEGVEIVPFLDRTKM
                     VNNAIGTVEKNLIEGALIVIFILVLFLGNLRAGLIVASVIPLSMMFAVIMMNAFGVSG
                     NLMSLGALDFGLIVDGAVIIVEAVMHRLYHSRHFETSNSITQQTMNDEVESSAGKMMN
                     SAVFGQIIILIVYLPILALVGIEGKMFKPMAQTVSFALIGAFILSLTYVPMITSLFLS
                     KKMHHRENFSDKMMKWIQRMYEPALHKALAFPKLITISVFLFFVFAVFILARMGGEFI
                     PKLEEGDFAVDTRLLTGSSLSQTIESTQQSAGILLKRFPEVEKVVTKIGSGEIPTDPM
                     PIEASDMMVILKDKNEWTSAKTFDELADKMSKALEDVPGITAGFQYPVQMRFNELMTG
                     ARQDVVCKIFGEDLDTLAAYSKKLGDVVSTVDGAADLYVETVTGLPQIVISFNRDQMS
                     HYQLNVADVNRVIRTAFAGESAGTIYENERRFDLVVRLNQQSRQDLTDVQNLLIATPI
                     GTQIPLYQVANVEIKEGPNQIQREDAKRRIVVGFNVRGRDVQSVVDELSKKVNEQVKL
                     PSGYYVTYGGQFENLVEAKKRLSIALPIALLLIFIMLYFAFGKLKYGLLIFSAIPLSA
                     IGGILALWLRGMPFSISAGVGFIALFGVAVLNGIVLITEFNRLKEQGMHDLKFIIMEG
                     TKVRLRPVLMTASVASLGFLPMALSAGAGAEVQRPLATVVIGGLVTATFLTLVLLPIL
                     YMWLEREKPRKRKARPVAVLFLLLSLGTVAQAQQPESKRIMLDELLKLADKQNITVQA
                     LQKESAYWKQLQSAVADQGRTLLSAEYGKINSINNDTRFTLSQSFTLPVVYKRQRAFY
                     SAGEQSQQAMVQWKEQELHREIKLLYWSMTDLLGRQQLLLQLDSVYSRVLQAATLRLK
                     AGETNQLEKTSTEAQVAQLKMQLAQIQSGLLTDQQQLQWLLNSNEFYLPDPVSVNNKE
                     MEWTDTSSISTHPLVKYREGQVKMATAQTDMEKNKLVPDLSAGYNNMSIVGYQSPDGV
                     TQKYYGPGQRFGTVNITMGVPLFTKAARNKVKAAKLSEEAAQLNVKAADQQLKMQLLQ
                     YQEAFKKQQQLVQYYNNEGLAQSALIIRHAGLNFEKGQISYLEWTMLMNNATGIQLAR
                     LDALAQLNRIRTEIEYLTGK"
     gene            33282..34415
                     /locus_tag="PLU11_00140"
     CDS             33282..34415
                     /locus_tag="PLU11_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN57538.1"
                     /translation="MNRINIYILAMLFTAACSSKKQAATEDKEAVLADQVQLTDVQLK
                     NAGIETGKAIRQPLNTVLKVNGHVDVPPQNIVSISFPMGGYLRSTKLLPGMHISRGEV
                     IAVIEDQSLVQLQQDYLVTKARLQFLQQEYERQKMLNENNVNAAKTFQQAESEYLSQK
                     VLLKGYSEKLFLIGINPAGLSENSISRSVSVHSPINGFVSKVNVNIGKYVTATDVLFE
                     LINPDDMHAALTVFEKDLNKVSPGQKVKVSFVDDPATTYDCEVILVTRNVDENRGALV
                     HCHFETQPKNLLPGMFLNATININNADVLTVPEEAVVRFDNKQYVFVQSGKNEFSMVE
                     VEPGVKENGRLEIKSKMEGFEQQLLVTRNAYAVLSKMKNTAEE"
     gene            34587..36866
                     /locus_tag="PLU11_00145"
     CDS             34587..36866
                     /locus_tag="PLU11_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPN57539.1"
                     /translation="MELKYSQQIAGTLSLTLKQVSSIYDLHTEGSTIPFIARYRKEAT
                     GNLDEVVIANVIDQIKYFNELEKRKETVLKTIEEAGKLTPELKRRIEDCINATELEDI
                     YLPYKPKRKTKASVAIEKGLEPLATLLFQQGPHNPEEEAAKYINEQVKDTTEALQGAR
                     DIMAEWIAENEQARNLVRKLFTEEATVSSRVLTTKKEEADAQKYRDYFEFKEPLAQSP
                     SHRILAIRRGEKEGFLIMTINIEKENAHAELKRVFLKASGAAAKQVELAIEDSYNRLL
                     QSSIETEFRMASKTRADEEAIRVFAENLRQLLLASPLGQKRIMAIDPGFRTGCKVVCL
                     DEHGTFLKYITIFPHAPQNDWNGAVQTIKELVARYDIEAIGYGNGTASKETEQLARGI
                     DFGKPVSVFMVNESGASIYSASEVAREEFPDEDVTVRGAISIGRRLLDPLSELVKIDA
                     KSIGVGQYQHDVNQNRLKEALDQVVESCVNFVGVDVNTASKHLLTYVSGLSATVAKNV
                     VEYRTKNGGFKSREELKKVSMLGPKAFEQCAGFLRIPNGANPLDNSAVHPESYHVVEA
                     MAAKASSSVPELITNPEVRKQIKAREFVSEKIGQFTIEDILKELEKPGRDPRSPIEEF
                     RFAEGVSTMEDLKPGMKVPGIVTNITNFGAFVDVGVKQDGLVHISHLSNRYISDPNEA
                     VKLGQKVMATVLEVDTARKRIALSLKDGQGSQEVRKSERPGDRRTGSPKDGKSGGAKP
                     QPLNPFQAKLMELKKNFKD"
     gene            complement(36968..37492)
                     /locus_tag="PLU11_00150"
     CDS             complement(36968..37492)
                     /locus_tag="PLU11_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN57540.1"
                     /translation="MTHYLIIPGLGNSGPAHWQTWFEKNVPDCKRIEQKEWDAPALSD
                     WLTAIDHAVAALDPSTVVLIGHSLGCSTIAHWAKHSGKIIKGAMLVAPSDIEAPVYTF
                     PATGFTPIPLEKIPFKTIVVASNNDVWVSAERARYFADCWGSEYINIGDAGHINADAG
                     FGPWEEGVSILKKL"
     gene            complement(37489..37803)
                     /locus_tag="PLU11_00155"
     CDS             complement(37489..37803)
                     /locus_tag="PLU11_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN57541.1"
                     /translation="MKQLKNNTPKNLAPGLTGYYAHGTNMTLGLVEIKKGSDLPQHQH
                     PHEQITYILEGQLDMTIDGKLCPLTAGMYYVIPSNVWHGAVAVTDCKVIDVFNPVRED
                     YR"
     gene            complement(37855..39051)
                     /locus_tag="PLU11_00160"
     CDS             complement(37855..39051)
                     /locus_tag="PLU11_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57542.1"
                     /translation="MVPSIREAYNKAFTQEKYQAFLEDLHSAWPGQIDFRVAETPVFI
                     PADFTRKVLDACENIVDIISDPKFNELTRNAIPEKVKVPGENDHSHFIAFDFGICVNE
                     QGEYEPQLIEMQGFPTLFGYEVLLDDVYSRHFPIPEGYSSYLGGHTRETYLQLLKEII
                     VGNHNPENVILLEIFPRKQKTRIDFYCTEEYTGIKMVCLTELIKEGKKLYYFNNGKKT
                     EIKRIYNRIIFDDLQQQSPEVQEKGKLLLEELDVEWCPHPNWFYRISKYTLPLIRHPY
                     VPPTFFLNEIKQLPADLENYVLKPLFSFAGQGVIIDVTAADIEAVKDPENWILQKKVK
                     YADVIVTPDIPAKAEIRIFYFWKDGSTRPMPTNNLARLSKGKMVGVRYNKDKEWVGGS
                     FCLFAP"
     gene            complement(39117..39962)
                     /locus_tag="PLU11_00165"
     CDS             complement(39117..39962)
                     /locus_tag="PLU11_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HPN57543.1"
                     /translation="MSWTEKRVVKVAVLDLYEGAANQGMRCIRNILIEWAEKNDLLLY
                     AKEYDVRLKNELPDTSYDLYISSGGPGDPLTSRYDDWDIAWCRWLDKMHRWNENPDNS
                     VKKHIFFICHSFQLASRFFNAGVVCKRKSTSFGVFPIHMLHSGKEERVFDGLKDPFYA
                     VDSRDYQVIQPHHSLLNEMGASILCIEKSRPHVPYERAIMGIRFNDYMIGTQFHPEAD
                     ASGMSMYLQREDKKTTVIENHGEEKWQNMLEHLDDPDKIVWTYSHILPNFLNHAVGQL
                     LEVPA"
     gene            complement(40215..>41813)
                     /locus_tag="PLU11_00170"
     CDS             complement(40215..>41813)
                     /locus_tag="PLU11_00170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN57544.1"
                     /translation="LLFACTSKQQADLLVFNATIYTVDTAFSTAEAMVVKEGKILATG
                     KVADLEKEFEAVEKLDAGGKFIYPGFIDAHAHFVGYGLSLQTVDLSGTKSWEECLERV
                     KSFAATNKEGWITGRGWDQNDWDMKEFPNNESLDTLFPDRPVLLARIDGHAAVANQIA
                     LELAGIKAGDTLTGGEVEEMEGSLTGILIDNAVDLVSSKIPASTAEQFKKALQAAQKN
                     CFAMGLTTIDDCGLSYTSVEQIKELQTAGELKMRLYVMLSDEKANYEYAKKNGPIKTD
                     RLNVRSFKVYGDGALGSRGACLLNPYQDKPGHYGFLLSQPAHFDSVASWLSEQRWQMC
                     THAIGDSGNRTILNTYAKYLKGKNDLRWRIEHAQVVNQNDFAIFGQYSIIPSIQPTHA
                     TSDMYWAGDRLGTERVKGAYAYQQLLQQNGWLPLGTDFPVEDISTFKTFYAAVIRKDA
                     KGWPETGYQMENALTREQALRGMTIWAARSNFEEHEKGSLEKGKLADFVILDADLLKE
                     VPEKLLAVKVKMTYVGGERVYERK"
BASE COUNT        10920 a         9394 c         9948 g        11451 t
ORIGIN      
        1 agacgacttt ttcgacgggg ttcatgtttt tcagcggggc atcgcttact attgatgccg
       61 acagtaagga agtggacctg ttgttttccc tcgaaggggc caatcgttgc tttgataata
      121 actccaccgc cgtggttcat tttgaaggaa ccaaagtaaa gatgaacctg cggaatgccg
      181 gaaccatgaa ttgtgaagga cttttccatt ttatctataa gaattcagtc aataccacca
      241 cccagctgca gaaactgacc acacaacggg tcagccagat cgtttttaca gacgccaata
      301 aaaaagagac gacggtgctc ctgactggaa aagaccagga gttgcttatt tcccttggaa
      361 cctgcctggt gactgaggct aaaacactga tccggtaatt tttatactac aacgaccaga
      421 aaaaacactt agccagaaaa aggttaacag ttaaaccacg gaaattttcg ccgggaaaaa
      481 gcgtattact ttaaagggtg aatactttta taaggagaaa taaccgaaag cgtaattgct
      541 taatatccgg ttcctttgaa tgcgctgaaa acataaccct atgaacggac aattaactgc
      601 aacgggtaat actattgttc ccgtatcact gaaccagctg atcaaatcac tggtagagaa
      661 tacacagcct ttagcaattc agcatcatac caaggtcgtt aatgaagtcg atggccggct
      721 cttgcttgga gaaggagcat cgcaactgag cagtgtgctg gaagagttgc tcgatgcggt
      781 catcctgaat tccagggaag gagatattca cattacggct gaccggttta aagacatggt
      841 gttgctgcat attgaagagc ggaataatta caacgggtat gccctgtcat tcagcgtagg
      901 ttcgctggag ccggatgcag cccggattgg aggacatatc agtatcgagg gtgaacggaa
      961 aaaagaagcc attatccagt ttagttttcc cggacaattt gccgcataaa attgaaaagc
     1021 cccatcaagg ggcttttccg taggttccat cacaggttaa ttatttacat tcattatacc
     1081 gtgcaatgat cttttccaga tcagtaaact gtagctgacc ggcctttgca ctatccatta
     1141 atgaagtgca atccccgaaa agattcacgg caaccgtatt aagattgtct tttgatacga
     1201 gcgtaagccc ggattgaccg ttagttttaa taaacaggtc gttgatccgg ccttccgtac
     1261 cagaactgat gccggctgct tcattcccat tatagatcac tttaccggtt gcatcagata
     1321 ctttctgcag gagactggcc ctggttccgg cagttactac cttgaagaaa tcattggcat
     1381 aactgatata ggcgacaccg cccacattca cttcattcac cgcatttccg ccataagtga
     1441 cttttttgcc gccggtctgc tgaaatacaa aggaggcttt ccctttgaaa ttttctttaa
     1501 tgaatccttc cagccggctg ccatccgccg taacgatata cccggccgta aatccggcag
     1561 gggcctgggc gaggagcggg gtaaacgggg tgagggagaa gaggagtgcg gtaagggcga
     1621 ttaacttttt catacttgct ttttttttat agtgttaaat ggttgattac ttactgataa
     1681 gtaagtcata ggggtaaaaa atttttaacc ggtcagctgt ttcatcaggc tgttgcagat
     1741 ggcagtataa tcctgctggc cggtaattct tgccagttgt actcctgctg ccaggttagt
     1801 catgatcagt atcgatttaa cccgggcggt ttcactgaag gtcagttctc cctttttctt
     1861 accttcccgc agtacttttt ccaccaatcc cagcaccagt ttcacgagtt cggtcagctt
     1921 cacctttgct ttttcaggca aggtattgta gtccgaacct accgaaccga tcaggcaaat
     1981 ctttccgtgc tcacaaaggg ccgtatagcg gttgataaaa gcctgaattt tctgacggga
     2041 cgaaataccg ggcttttcca gcgaacgggc cagttcatga tagcgttgaa tatactgttc
     2101 aacgatttca gcaagcagat cttcttttcc agggaaatga taatggacag ccgcgttttt
     2161 tattttcagt tctttggcta tatccgcata actgaatgaa tgatacccgt aggtctgaat
     2221 gaattccatc cctaatcgca ggatcgattc cttggtatcg gtaggacggg ctgccatgga
     2281 tcaaatatac tgaaaactta ctaagtagta agttatttga ccggcataaa aaaacccgcc
     2341 ggttggcggg ttttcgctgt atgtgaaaaa tgattaataa cggtaaagtt cagatttaaa
     2401 cggacctgct ttcgtgatac caaggtactc ggcctgggca tcggtcagtt catccagaac
     2461 caccccgatt ttggcaaggt gaagacgggc cactttctca tccaggtgct tgggaagcac
     2521 atatacttta ttctcgtaat tcttgtgatt attccagagt tcgatctggg ccagcgtctg
     2581 gttggagaat gaattactca ttacaaagga cgggtgaccg gtagcgcaac cgaggttcac
     2641 caggcggcct tcggccagga tgatcacatc atgtccgtcc acattgtaaa tatccacctg
     2701 gggcttaacg gtgtctttgg tatgaccgta gttggtattc agccaggcaa tgtcgatttc
     2761 gatatcgaag tgcccgatat tacaaacaat agctttatct ttcatcagct tgaaatgcgc
     2821 acccgtgatc aggtcgcggc atccggaagc ggtaaccacg atatcggctt ctttcaccgc
     2881 atcgatcatt tttttcactt caaaaccgtc catggctgcc tgcagggcac agatcggatc
     2941 gatctcggta acaatcacgc ggcaaccggc acctgccagg gaagcagcag aacctttacc
     3001 cacgtcacca taaccaccga caacagctac tttaccggcc agcatcacgt cggtagcccg
     3061 gcggatagag tccactaatg attctttaca accgtattta ttgtcaaatt tggatttagt
     3121 aacagagtcg tttacattaa tggctggcat cggaagggta cctttggcta ctctttcata
     3181 gaggcggtgt acgccggtag tggtttcttc agaaatacct tttacatgct gtaccagctc
     3241 agggtaagta tccagcacca tattggtcag gtcacccccg tcatccagga tcatattcaa
     3301 cgggcggtca gcggcaccga aaaacagggt ttgctcaata caccagtctg cttcttcctg
     3361 ggtttgtcct ttccaggcaa atacaccaat accggcggcg gcaatcgcag cagcggcatg
     3421 atcctgtgtt gagaagatat tgcaggagct ccattttact tctgctccca gggctgtcag
     3481 ggtttcaatc agcacagcgg tctgaatggt catatgcaga caaccggcaa cgcgggcacc
     3541 tttcaggggc tgggaaggtc cgtattcaga acggatggcc atcagtccgg gcatttctgc
     3601 ttcggccagg cggatttctt tacggcccca ttctgccagt gaaatatctg ccactttata
     3661 gggcaggctg aagtcgatgg attttgatac agttgacatt tttgcgtata tttttatggc
     3721 ggcaaaggta ctattattta ctgtaaaacc cttgctgaat aacggtttca gcctatttat
     3781 tttaggtctg tgttcaatac ctgctcaggc ctaaccgctt attgttcaac aggtacggta
     3841 tttccggccg taaaagtaaa agtcccgccg ctgatctgtt gggcatccgg gagatgtatc
     3901 agcatcctga agaccataaa tggcataatt cagtacccgg ctgcatgaag aaattaattt
     3961 ttgcggtcaa aaagccgttt aagcaaacct ttcttttttt taggatcttc ctgggccgat
     4021 ccaatgggct tagtttccgt ttcttttttt accggcgtgt ttacaatact gtctcttttg
     4081 gctggcatat tatcttcctt taccggggtg gattccgggc tgatatactc attattattc
     4141 agctggtaat cttcttcggt accggtaccc tggtctacgc cctcggcacc cggaaccgga
     4201 tcctgttcac taatgatgat atcggcatta ttgggacctt cttccaaatc agcgggtttg
     4261 ataaattttg cgtccttttc aattcccagt tttttgtcgg caagtacttt ctggaaaaaa
     4321 tattcggcaa tgggtcgggc aatgcggctg ccatcgccct ggttgtgcat aaaggtatat
     4381 tcgaaaccca cccaggatcc agccagtaac tgtggggtgt acccgataaa ccaggcatcc
     4441 gcattgtcat ccgtggtacc tgttttgccc cccatttctg cagcaccaac ccgcgtacgc
     4501 atgcctttag ctgttccttt atccacggtt ccctgcatca tccgggccat ggtataggcg
     4561 gtggcttcac tgattacttc tttccggttt acactaaaat caaagcgttt gatcacattc
     4621 ccattccggt cttcgatccg gctgatggca tagggtttgg tggagaatcc acggccggca
     4681 aacatggtat acgaccagag catttcatac atcgacagat cacaggtgcc cagggcgatg
     4741 gatggataag gttttacagg agtcggtatg ttgatccggg ccagaaactc ggaaaactgt
     4801 ttgggttgta cctgcttcat gatataagcg gtggcacagt ttttagaaaa tgccagcgca
     4861 tcagccatgg tcatagaccc acctccacat tgttttccgg aaggcaccca tccactgccc
     4921 tggaagaact gggccacatt ttcacattca gtggtaggcg taaagcccct ttcttccatt
     4981 gcctgagtat agagtaatgg tttaatcgtg gagccgacct gtcttttcgt tttcaggttg
     5041 acgtggtcat ttttataggt cttgaaatga atacccccca cccaggccct gatctcaccg
     5101 gtcaccgggt ccatcgcaat aaaaccggcc tgttccatct gcaaatgata tttgatggaa
     5161 tccagcgggg tcatgacggt gtctttttcc cgtttgttat tccaggaaaa aatcttcata
     5221 ggtactttaa cattaaacga agcttttatc tctttatcac ttagtccgtc ttcttccagg
     5281 tttttccagc ggtcggattc tttgattgct ttctccagca gtttttcacg tcctttccag
     5341 accgttccgt ttttcatatc cgtacgctgg ttgatcccct tttgcagcat ggccatgtgc
     5401 tgcgccatgc cttcttcggc atattgctgc atttgtacat taatcgtggt atagatcttc
     5461 agcccgtcat tataaatact gtatttgccg ccatccggtt tctctacatc cttgagggct
     5521 tccttaattt catctttcag tacttcccgg aaataaggcg cataaccggt attttcatcc
     5581 agtttacggt aattgagttt aatatgaagc gcttttgcct ttttggcttc agctgccgtg
     5641 atcttcccgt ttttttccat ctgactcaat acggtgtttc gtctgtccag ggccagtttg
     5701 ggattacgtt gcggattgta aatactatta cccttcagca tacccaccag caatgcagct
     5761 tcttccaccg ttaaccggtc cggctctttc tggaaaaaag tccgggatgc attccggatt
     5821 ccgtatacat tattcccgaa aggcaccgca ttcaggtata aagtgatgat ctcttctttg
     5881 gtgaaattcc gctccagttt aacggcaatg atccattctt ttaatttctg aatcacccgt
     5941 aacgctttat tcgttgcccg ttcattaaag aggttcaggg ccagctgttg ggtgatcgta
     6001 ctgcccccgc cttcggatcc cagggtgaaa acagcccgca tagtggaacg gaagtcgatc
     6061 ccgctatgac tgtaaaagcg ctcatcttcc gtggctacca gtgcatcaat gacatagggg
     6121 gaaatatccc ggtatttcac attgctgcgg ttacctcttt ctgtataata tttacccatc
     6181 agggttccgt cttctgcata aacttcggaa gcctgcagaa tggagggatt ttgtaattca
     6241 ttaatagagg gcatggcacc aaagacgcca aaattggcca gcacaatgac cagtataaaa
     6301 gccgcaaggc cgccaaaaaa aatacgccag aaaatccgta ccgctttttt catatgatga
     6361 tgaatggttg aggtttgtcg atcttataga ttttatcgga gaaccgaaaa taagaaatta
     6421 ccggaggggt gggggaaacc agtatttaat tttttgcgaa agtgtgcctg aaagggaggt
     6481 tttacccgta tctcccgctt tttcatcaga atttacccgg caaatgctgt tccaggaaac
     6541 gtttgtatgc ttcaaggtcc ttacccgctt tgagtaattg caggttactt tctgagataa
     6601 tcgtaaagta atatttacct ccttttaacc agggaataat atctgacgca gttttagggc
     6661 gtgttttttc cacgtaagca actgcttcat cggtattttg gaagaaactc atcagcaaca
     6721 ggcggttttc ctcatcaagc tgatacaggt cggccgtata cgttttgtta taataggttt
     6781 ccctgttata ccggaagaag gcatttttag cctcatttat aaacacagga tccactttat
     6841 tcagcaccag gaccacaaaa tatttgtcag ccggtttaaa aacaaaaggg gtggaagtaa
     6901 caacaggtgg tggtgctaca ggctgtgttt tcaccgtatc ctgtacaggt tgcttaacgg
     6961 tcgggggcat gactgttgcc ggaggcttaa cggaacccgt gtcagttttg gttacgggtg
     7021 gagcgggaat cactgtcacc ggcgccactg ttccggaatc cggataacgg gttatctgga
     7081 gattagtcag ttcttcttca atctgggccc tccggctgag cacatcaatc aggtcaatcg
     7141 atttttcatt cagcggggat ttaggaaagc gggatatgat cgaattcaaa atatccttcg
     7201 cttcattatc atcccgttgc ttaatataat agaccgcttc aatgtagagc agttgtgggg
     7261 tccagtaatt attcccatac agactgtcag cggttctttt catggctatt gcagcggcga
     7321 aatcgccttc aatgaaaaga tcatagatct gttcataggt ttttttagct gcactgtttc
     7381 ctgtttcatc tgccggattt ttaccggtaa gtacaatcgt ggtaaggtta ctggcggcaa
     7441 atttgccctc catcagtttt ttaatctcag cggctttacc agtttccccg tttttattgt
     7501 aacaataaaa caggttaaac agcacttcat ccattggcat gaaatcggga tatgttgccc
     7561 gcagctcctc aagtgttgcg gtacccgcac tgcaatcctc aataccctga atatatgttt
     7621 tacccaggtt gaacaaggct ttctgtatag aatcattgga ttgcgccagt tgggcttctg
     7681 ttaacggaag acgggcatac aaatcgtcaa aactgattcc gttactcatg tcgccgccac
     7741 tgttgctccc gggatcctgt ggattactac cccccgggga ggcattcatc cggttattca
     7801 ggatcgcagc gcctcttctc caattgtctg tattgggccg gttgccccat ttggccctga
     7861 attcattttg tcctcttgaa cgggcattgg tattataaaa gtaccattcg cccttggtgt
     7921 caccggttgg gaaaagggta accggtggtt gattaccggt tgtttgtatt ccagttgtca
     7981 ggatgggctg ttcatccttt agtccctgtt gtttacgcag gtatttaacg agttttttta
     8041 catagtcacg acgctcatcc tctggtagag ccgcgatccg ctgcagactg tcttgtcttt
     8101 ccctgatttc aatattgacc acgatctccc gtaaagctgt tttcctggaa gaaattgcat
     8161 ccggattggt aatggaagga tcgtccagct tgatgctgtc ataataggca tacgcgtttt
     8221 tatatgcttt ttgcaaaaag gacagatcac ccatttgcag aaatgcttta ttcttttgtc
     8281 cgggattatt attaggtgcc tgtgtgctga ggagcagcaa ctgaagagcc tcctccactt
     8341 tatttccttc caactgcatc tgggccgcca tgtagtaaat tatatcctgg taatcggcat
     8401 atttatcacg tcgggccatt tttaccagtt cagccacatt tttctgtata tcagcctctc
     8461 catcatcctt attggtgcgg attgttgcca ggcgggcgta tatctccatc accaggtcgg
     8521 tggtacggct aattgaacga tcataatttt tccgggcctc tttaaaattg ccggccattt
     8581 cataaagctg accagccagg tattcccaac gggctctttc ctgttggttg ccggcattgc
     8641 tcagggcctc aaccaggtga aaggcggcac tatcccagtt gttttgtttg taaaacgaat
     8701 aagcctgcac ttcatgcagg tcattcatca aacgggatgg aaaggccggg tcatctcgaa
     8761 gggtaacgat cagactggag gcttcggcaa actggtcctg ggccaggaaa ttccggattt
     8821 gccagataaa tgcatcgttt cgactcgggg gttctgtgaa catttttttc gccagtcctc
     8881 ttttttcccg ggtagaaatg ctcagggctg aatttccgtc acgggcactg ccgattgttt
     8941 tatagtagcc atctttttct ttaggggcaa aagcgtaatt gataaactga aacatctgat
     9001 aagccgagtc aaaatccttt tgcaggtagt aggaagctcc ccagaggagg tagagattgt
     9061 ccacccagtc attcctgagg tcatgtaagg caatacccga ggatgctttt aaggtgattg
     9121 agtccagttg taatgaatcg gctgccgtaa catccagcga gtaattgtaa aaaggcaata
     9181 gtttggaata gtcgtcttta aacgcctgtt tggccctggc caatacatca ttcagtttgt
     9241 tattggcatt aaacgtgtaa ttgtagtgcg ttacggtatt ttgcacaaaa cggcggggaa
     9301 cagtgaattt tttatcggtt ttttccgaac ggagtacccg ctcctcgtat tcctttggtt
     9361 ttttaatatt caggtcaaag cccagctgac caatagcagg tagaccggcc tgtaaaagga
     9421 taagggagaa aaggaaaaaa atgatatgtc gttttgctcg catttgccgg cgcaatctaa
     9481 agtcttaata actgaatttc aaataattta gtataaaaat aggccaaatt attttgacct
     9541 gaattcgccg gaaaagcagg gttcatttac tatctttaaa caccaaaaat ccttttatgg
     9601 ccaaattaga agccggctcc accctgcaaa ggctgagaaa ccgataccgc ctggtggtga
     9661 tgaatgacga tacttacgag gaagtggtca cgtttaaatt gtcccggctg agcgtttatg
     9721 tcatgttgag cacaatattt gtagtattgg tcggacttac agtcgccctc atcgttttca
     9781 cacctttgaa gtactatata ccaggtacaa gtaatgatta taagtcagtt atggagctaa
     9841 aacggcttca gtaccgggtc gattccgtgg aaagacagga tgcctataag acccagtata
     9901 tactgggtct gaaaaaagcc ctgggaagta cggaaaccgc taaactggat accacggcca
     9961 tcgtggtgcc caaaccggat atttctaatg actaatgata aaaccaatgt tcaacaacaa
    10021 aaacaaaacc gacatgcagc aaccagtatc caatggtaat ggcgggggcg caaccctgat
    10081 cagtgcaggc actgtattaa aaggcgatat cagcagcagc agcgatctgc ggatcgatgg
    10141 caccgtaatc ggcaatatca aaagcggctc caagattatt gtgggcagca gcggggtggt
    10201 ggaaggggat atcaccggca accaggccga tattaccgga aaagtaaacg gcaatatcag
    10261 ggccaaggag cttcttcagt taagaggaga gtgtcttgta tccggaaata tctatgccgg
    10321 caaactgcag gtagaaccct ctgctacttt caacggtcaa tgccatatgg gggccaatat
    10381 tgtagagatg ccaaacaatg aatcagccgt caccgtcgcc gcagcgaaat aacaacaaag
    10441 aactactccg gtatgcaggc cttggcactc agctgctggc agctatcgga atcgctgtat
    10501 ttgccggaat caaagcggat aaatggctgc atacctttcc attgctgtct tgtgtcttac
    10561 ctttggtcgt cctgtcggca ctgttttata aactgttccg ggaaaccagc aggtccgata
    10621 aaaaatgaac agatcactgt tgcaacctat tcgtcctttg tttttggtat ttatccttgt
    10681 caatgcactg gccctgaccg ccaaatccct gctggagaaa taccatttca accaggaact
    10741 gctgatcctc ggtaacctgc tcctgtttgt ggtgatcctg gtttcctacc tgatcaccta
    10801 tcgttcactc cattcgtcca attctcaggc ttttatacgc gccatgtacg gcagtttcct
    10861 gattaagttt tttatcctgg ctattgcggc ttttacatac ataatgatcg ccaaaaagga
    10921 ggtgaataaa cccggactgg ccgtgtgtgc actgttgtat atcatctata cggccatcga
    10981 gatcagggcc ctgatgtcaa cacttaaaag aatgaagcag cagtcgcatg gctaaaaaag
    11041 aagtccctgt caatcacctc gaacaatttc ttccaccggg tacgggggaa gcagtgctgc
    11101 attacctcca tgaatacaaa gttcacctga ccattgcccg tgaacgaaag agtattttag
    11161 gggattaccg ccaccggacg catcagcaga atcaccgcat cagcgtaaac ggcaacctca
    11221 atccctatgc cttcctcgtc acactgctgc atgaaattgc ccatctccta acgtttgaac
    11281 aatatggcaa ccgggtgaat gcacatggcc gggagtggaa atcagttttc ggaaaattat
    11341 tgcatcagtt tgtacaaaac caggtgttcc ccccggatat ccagcaagaa ctgactgaat
    11401 ccctcaagaa tccagctgcc agcagttgtg cggaagacgg actgctacgg gtcttaagac
    11461 gctatgatgc aaagaaggat aatcatcacc tgatcgaaga attcccaatg ggtagtttat
    11521 tccgcattaa agatggacgg gtattcagaa aaggagagaa gcttcgcaaa cgctataaat
    11581 gcactgaact gccctcggga aaagtgtatt tgttcagtcc ggtatatgag gcggtgcttg
    11641 tgacagttag tagttagcag atagtagtta gaagtccgat gcttcggaca acagcccctg
    11701 ctgaagggtg ttggttgaat attttttaga aaaacatctt tggaaactgg tgcttttcat
    11761 ttagattacg tttcttagca ggcccttctc gcttctagct tctcgcttct agctctttca
    11821 tcatccacaa cgggcaacca aaatgcccgc tattacccat cggtccgctg aggtaggaaa
    11881 agcctaagcg ttcatatact ttcaacgcag ctttcagttc gttcatggtc tcgagataaa
    11941 ccttactgaa accattggcc gctgcctgtt ccagacaacg gttaatcagt tgtgtgccta
    12001 aacccgtgcc cctggcttca ggaagcaggt acatttttac gagttcacag gtatctgccg
    12061 gtaaaccagc cgtcgggaaa atgccaccgc cgccaacgat ctttccatct atttcagcaa
    12121 tattatacac cgatttttcc tgttggaata attcaaacag tgtgtcggtc gtgggatcgt
    12181 agtacacagt gcccgggtga ttggcgccaa attctgtcag cgtatcgcgg atgattttgg
    12241 cgatgaccgc gttgtcggcg ggttggatgg ggcgaatggt ggggggcatg gcgcaaagct
    12301 agggagaaaa ggtgagtggt gagtggtgag tagtgagtgg tgagtggtga atagtgagta
    12361 gtgagtggtg agtggtgagt aggatttcta agagtgattg tcatcccaac cgaagggagg
    12421 gaactaaata accccatccc ggaaacggga cggggttcac catctatgaa accaaaacca
    12481 actgttagtc gagctgcaag actttatcgc ggggatattt cacttcgtat ttgaagccca
    12541 gtttattttc ttttttcgga tctaccgtca gttgccaggt cagtttctgg gtgtcatcat
    12601 ccagtttggc tccgtcatat ttacgatcct ggatctcaat ctccttcatc gtgctgatgg
    12661 gaaactggtc atcgattgtg atactgatgg gttgctgttt attattgcgg accacgattt
    12721 catattgacg ggtatcggtt ttgttgctgc ccatgaattt gcgggaactg tattctttca
    12781 gcaatgttct ttttaccacc acgcctttgt cacggccgag ggagagattc agcgtatcac
    12841 cggcattcat cacatccaag gtggatttac cgaggaaagt accttcgaaa aacaggttgg
    12901 cttccccggg cagcaggttg agttcctgcc agtcggcaat acgggcggtt aagaatgcat
    12961 cgggttcaaa tttgggggca gcataatatt caaattgagc tttcagttca tagccgtcaa
    13021 tatccacggt ataggttttg ccgtcattca gtacggtgta gggatcgtca atttcataga
    13081 tcgtggtggt aggctgataa ctggtggtgg tattgatcgc agtcagttct tttttcttcc
    13141 ggtaattagg ggatgctccg ggttggtaat cgtcatagcc gctgccacca ctatagccaa
    13201 taacaaccac ttctgaaagt gcattactgc tgggttgaag ggtgatgttc atgaagcctg
    13261 agttggcata atattccgcc cgttccattc ccaccgcgct gatctcaagg accggattgc
    13321 cagtgccggt ccggatggaa aaattcccat tggcatctgc tgcagccccc gttttagttc
    13381 ccttgatcag gatacttgcc ccgggcaccg ggttgccctg ctgatctgta atcctgccgg
    13441 aaaccatgcc cggtgtattc agactcagcc cgggtacccg tccctgtaat gggttgaccg
    13501 atgccgtcgg ataatagtat ttcaggaacc agggattgat cactggtttg gttccgtctt
    13561 cattgggatt gccggtagat aaatacagtt ttacgtcttt ccaatcctca ccactctgct
    13621 gcgatacatt ggctttatac tgcaggttga tcggactgct gatgtctttt acgcggatat
    13681 cataggtcgg ataccatccc gatttgcgga ccaggtaggt gagggtgaac tggctgttgg
    13741 tggtttcttt ggcattcacg gtaacatgga tctcgctggt ggatatatcc gctttctgat
    13801 ttagttcggc caactgttta ttgagtttgg ccatttccgc atcctgtttt ttgatcacgc
    13861 ggtccgtttc catttgtttc tggtatactt cggtcagccg gttccgctgg aaatcggccg
    13921 cctctttcag gtccgcagct ttcagcccgg tattggcgcc gccgatctgc tggtttttca
    13981 gcagcatagc ttcttcctgt ttgaatacat tgaggatatt ccgttgcagg gcgatctttt
    14041 ccagtaaggc ttctttctgg gtttccagtt ctctgatctc ttcctgcttt tgctgctcct
    14101 tcatgaaatt ctgctggtga atcaccgata gaaccgtgat cgctccatct gcttttacct
    14161 ggatgctctg ggcgtcaatg gaaggggaga tggattggaa taccagttca tatttgcctg
    14221 gtgtaaggct cacttttgca ccccgctggg tctgggctcc ctgcagaaag acggtgacct
    14281 tttcaatttt actgtccact gtttttttgg tctgtgcatt gacctgcaga atacataagc
    14341 aggccagtga catgatcaac tgtctcataa tagatggttt tgagacagga tgcgaagggt
    14401 gtccggatta catacttaat tttcccggtc attaatatta taccggatgt ctgttaatct
    14461 gaattttccg tatacaaatt ttccatccgg gtaatcccag acggcatcgg cttctgatac
    14521 cagattaaaa cccctgactg gccggtattc gtgaatgggc gtggagaatg gtattctttt
    14581 atggtccgcg atagccatcc ggtcgttgga gctgaaattg atcagctgac cagctttatt
    14641 aaagtaaagg gtggcactga tgctgatccc ccgattgata aaaacagccc ggaccgtgag
    14701 tgaatcgata ggttcccaac tgatccggga gtcgatcagg gaggccggtg ccagcaaaca
    14761 catatcattg aatagggtga cggtttccgt agtgtccata tccggtcctg attggtgaat
    14821 caccggtatt tttccaaaaa ggcggatatc cattgtggcg gaagcatcca cataccggtg
    14881 atagccgggt acagtgatcc catacatggc cgccttcata aagaataacc tggcggggtt
    14941 ttcaaaaaaa ttatactgtt cagaccggaa tgaaaaaaaa tccttccctt tttcccgcat
    15001 ttgtccttca aacatcacct gcatattcag caactttggc tgattcaata caccgcaata
    15061 aatcaggtag cgttgtaccg gaaccggtaa gtgcccgata tctgcactgg tcagaagtgg
    15121 tatggcattc agggagttct ttttcactaa ttcagcggct tcttttttat acgactgttc
    15181 aaacaaacgg gtggtcaggc tcagcaaggc aatcagtaaa agaataccat tggcaatcgt
    15241 accatattta gcatccttcc agctcagcac aataataacc tgtgagagga ggcaggctat
    15301 gatacaggta tacatccacg aaccatactt taacaggtaa agtgtggccg atattaaaaa
    15361 cagcaaagca gtcaacagcc ataagagccc gataggtttt gatatagaga tactcagctg
    15421 tttcatgtca gccagcccaa aagctttact aaagcccata aaatgaatga ggccatgaat
    15481 caggatcagg gcaacaaata agtagcgcat aggaaagagt tgaatcttgt aaggtagann
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gccctggaga cgaacgatga
    15661 tcggaatatg aatgttgccg agatttttgt aggcatcaat gacaccctgg gccacccggt
    15721 cgcaacgaac gataccgccg aagatattga tgaggatcgc ttttacagca ggatctttca
    15781 ggatgatttt aaatccggct tccaccgttt gcgcattggc cgtaccgcct acatccagga
    15841 agttggcagg ttcaccaccg ctgagtttga tcatatccat agtggccatg gccagaccag
    15901 ccccattcac catacaaccc acattgccat ccagtttcac aaagttcagg ttgaactgac
    15961 cggcttccac ttcagtcgga tcttcctctg taacatcgcg cagggaagaa agatcggcat
    16021 ggcgtaccag ggcgttatca tccaggttca tcttacagtc aacggccacg attttattat
    16081 cagccgcttt gaacagcggg ttaatttcca gcatggagca atccagccct acataagcat
    16141 tgtaaagatt ggtcacaaat tttacgcaat tcttaaatgc gtctccgctc agtcccaggt
    16201 tgaatgcaat tttacgcgcc tggaatccct gaagtccacc ggaagggtgt acccattctt
    16261 taaaaatctt ttcgggggta ttatgggcta cttcctcgat attcataccg ccttcggtac
    16321 tgtacatgat tacattctga cccttggtac ggtcgagcag gatggagaga tagaattcct
    16381 tacggtcact tataccgtca taatacatat cctgggccac caatatttta ttcaccactt
    16441 taccggctgg tccggtttgc agggttacca gggttcctcc cagtattttt ttcgcgaagt
    16501 ctgcgatatc ttcaatattc tttcccactt tcacaccgtt gatcccattt tctttaatgg
    16561 ctcctttacc gcggccgcct gcatggatct gagcttttac aacggcgaag ctgctgccga
    16621 actggttttt gatctggcgg taggcttctt ccgcttcatg tactgtactg caggcaaatc
    16681 cttcctgcac cggaacattg tattttttca gtagttcctt cgcctggtat tcatggagat
    16741 tcataatgga aaaatttgcg caaagataaa ggaacaggga cagtgaaagg cgattaggga
    16801 atagtttttt taccttgcag gcatgcgact gtttccaata ttagccgttt tttacctggc
    16861 aggctgtcat ccgggttaca gaatgcaatg gcccgaaaac aggacagggc tgaccggttc
    16921 aactttttac gagcaggcag cggccatgca gtgggccgcc cgtgattccc tcgttgttca
    16981 ggctgccctt tcgggcaacc tgcccccttt tctccggaag tttgtaccag tcagggtttc
    17041 cctcacagat agcctctccg gcaagatgat caaggctgtt ttttatgtcg ccccagacta
    17101 cctcagtatc ggttccggtg acgactgggc gcgcgtctgt attacgccct atgcggctca
    17161 acggatagcc gacagcctta attgttttct gcccacccgg aaaatggtgg atgatattta
    17221 ccaggcggca actctgaaac tcagaccagt ccccctcttt gcttaccggg acagcacccc
    17281 gtccatgtgg cagcatcacc tgatcatcga aggtcagcga aaaggcaaaa aaggactgat
    17341 tgccggtatc aaaaaagatg tcgtgatttc cggaaagatc agccgggata aaaagccgga
    17401 ccgggtagct atttatggct ggcatcaacc gggaggccgt gttatacaac ctttatatac
    17461 cggtcatata tattggtggg tggattacag ccagggaatc cggctggttt accgcaggat
    17521 caatgtggac gggagatgga tggattatac tgaggtgtta aaaaatcccc ggcttagccg
    17581 cttattatgt gatgaaaagg actgtgattt tttgcggtac cctgtacacc cataacctga
    17641 aaggacaaca ggaacagact tattatttta attaacagaa taaacgccga tgtttacctt
    17701 tgcagcgcta ttttgcaacg ttatttcatc ttttatcaaa tcagttttac tctgagaagc
    17761 aactatatat acctgtctct ttttttactg ctttttattt cccaggcctt gctggcacag
    17821 gagcctgcta tgcaattggg aaaccggctt aaaaatctgg gaagcagttt ctccggcggc
    17881 agtggaaatg acagcctgaa acaccgggat aattcagagg attccatcac gatcagtttc
    17941 cggtatatgg attctacccg gcagtacaaa ctcgattcct cggtcggcga ttttacccgt
    18001 cgctttccgc tcccgggaac ctcgatcaac cttgggaata ccggcctggc cacccgttca
    18061 ttgctttttt ctccggaatt aaatgctggt tttgatccgg gttttcacgc ctttgatgta
    18121 tataaatgga aactgagtga agttcgtttt tttaatacca cccgtcccta ttcagaactg
    18181 aattatttac tgggcagccg ggtggaacag atgatcagcc tggtccatac ccagaatatc
    18241 aagcccaact ggaatttcct gtttcagtac cggctgatca attctcccgg gtctttccag
    18301 aaccagaaca gcaatcataa taattacctg ttgagttcaa agtatcaatc gaaaaatctg
    18361 cggtacaata attatttcgt attgctttcc aataaacttc aggcctatga aaacgggggc
    18421 attattgata ccacggatat cctgaacgat ccggacttta aggaacgtta taatatcaac
    18481 acgaggatcg ggggtaaaaa ggttttttcc accaactttt ttggaaataa aatcaataca
    18541 ggaaataagt acagcgaaac caccctgctg ttccgtcaac agtatgattt tggaaaaaag
    18601 gattccctgg tgaccgattc cactattatc ccattgttct ttccacgact gcgttttgaa
    18661 cataccctcc agtttgatcg taacagttat acttttcagg ataatgcagg ggattcactt
    18721 tattaccggg cgtactatga tactgcactc agagatgcca atgatacagc cctgttttcg
    18781 gataaatgga aattgctgaa caatgatttt tccatttacc agtttccgga tgccaaaaat
    18841 ctgcatcagt ttattaaact gggattactg gtacagacga tcaccggcac tttgtcttcg
    18901 ggtaaactga atttctttaa tacggcggga catgcggagt atcggaataa aacaaaaaat
    18961 cagcgttggg atatccaggc caatgggcgg ttgtttttca ccggttataa ttccggagat
    19021 tatgaggcgc atatccgtct gcagagtctg ctgagcaaaa aagccggatc gctggaactc
    19081 ggatttgaaa acacgagccg gactccctcg tttatgtttg attcccggag cagtttttac
    19141 ctgatgaaat cagtggtaaa cctgaaaaag gaaaacaata ctcatctctt tgcctcttat
    19201 tttcttcctt catttaaaat gaaactgacc ggtcattatt acctggtcac gaattatacc
    19261 tacctggata attattttga agtaaatcag gaaagcagcc tcttcaacct gcttcagata
    19321 gcagtagaga aaacgatccg gttcggcaaa cactggaact ggcatgccga actgtatttt
    19381 cagcaacggg taggagaggc cccggtcaat gtgccggcct tatatacccg taaccggatc
    19441 ggctacgaag gaagccttgg gtttaaaaac ctggatattg cctttggcac ggagatcagg
    19501 taccgggctg cctataaagc ggatcattat tcaccggtaa tgggtcgttt tttctatcag
    19561 gataccacgg tcatccggaa tccattgccc gatattgcgg cttatgtaca tttcagaatc
    19621 cgacccttta ccgcctttgt ccggatggaa aacctcaata ccgcccggaa tttcaacgga
    19681 ttcggtttta cccgtaacaa cctggtagct cccttttacg cccttccggg cctgcagttc
    19741 agactgggcg tattctggcg ctttgttaat tgattctcaa ataattgacc ctgataaaac
    19801 tcagttagta ttcttcaata ttgcgagtat ctttgtaaag acatgaagaa actggtcctg
    19861 accatatctt ttttatgcta tctcacggta acctgtgggg tagtggtcaa tctgcattat
    19921 tgcatggacc ggctggcttc tgtacatttc ttcggggatg aatcagacca gtgtggtcag
    19981 tgcgggatgt tgttacatga aagcaatacc tgctgtcacg atgaagtgaa agtttttaaa
    20041 ctggaacagg atcagaataa agtcagtatt acggattata cacctgtttt taccactgaa
    20101 ctgacaattg aaccttcact ttttctgaat gctccgtttg aatcggagtt gggactggtg
    20161 aacacctttg tcaatccacc tccactatta tccgaacagg atacctatct gcagattaat
    20221 gtcttccgta tctgatacat taattatttt atagcgacag gcaacagccg gtcaaccttt
    20281 gtgtcttatg gcacgatatt atttattcat tatttaataa agttttttat gaaaacgata
    20341 cgcttatttt cattgactgc cgttatggta gccattactt cactgggttt tgcccagact
    20401 aaaacagaat cattccaggt ttccggaaat tgcggtatgt gcaagacccg tattgagaaa
    20461 gcggctaaag atgccggtgc gtccgaagcc aaatgggatg cggctacgca acaactgacc
    20521 attacttata ccagttccac caccaatgcc gctaagatcc aggagaagat agctgccgtt
    20581 ggtcatgata atgccggttt caaatccacc atggaagtat acaataagct gccgggttgc
    20641 tgcaaatatg ataaagatgc agcctccggt tcgatggatt gctgtaaaga tggcaaatgc
    20701 accaatgccg gtcattgcga taagccctgc tgtaaaaaag atggcgacat gaaaatggat
    20761 tgttgtaaag atggcaaatg tgataaagaa ggacatgccg gtaaggattg ctgcaaaaaa
    20821 gaaggtgctg ccaaaatgga ttgctgcaaa gatggcaaat gcagtaagga aggacatgat
    20881 ggtaaagatt gctgtaaaaa agaaggggct gccaaaatgg actgttgcaa ggatggcaaa
    20941 tgcagcaagg aaggacatga tggtaaagat tgctgtaaaa aatcctgatt aagcgaaaaa
    21001 acatacttgt gaagaaaaca gggttgttga taataatggc gttacaggta ctgctgatac
    21061 aagcgcagtt cagcaaggcg aacctgcagg ctaccggtct gacctgtgcc ctgtgcagca
    21121 atgcaattaa taaagcactg aagcaacttc cctttgtgga atcagtgaaa gcggatatta
    21181 agaactcctc tttcagtatt gtattccggc agggcatgga cgtaaaaccc gatgcgatca
    21241 gggatgccgt ggagggcgcc ggtttctttg ttggtggatt acagttaact ggttcattta
    21301 acaacctcag taccggtccg gatggaatta tccgtttagg aacgaactgg tttcgttttc
    21361 ttaactcgtc taacaaggtg ttgaacggcg aagttacttt taccctgcag gataaatcat
    21421 tcctgacagc caaagcgttt aaaaagatca gcagtacggt aaagatcccg agcttgtcaa
    21481 ccgggaaagc agaagcagac caggttaaag ccggggtgaa aaaaggggag cggatctatc
    21541 acgtgaccat ttgatcagta tatctggttt ctctccccgg tattccgggg agagaaactt
    21601 tttaaagtgt ttatatgttt tcattgaaac aaattatcgt tgtattcttc ctgttgtgta
    21661 tgagtacact ggcgcaggcg caaatcaaac aagggtataa agcacctgaa gtagcattgc
    21721 ccgctgccaa cggagatacg atccggcttt catccttgaa agggaaagtg gtgttgctcg
    21781 acttctgggc ttcctggtgt ggtccctgcc ggatttccaa taagggattg gtgaaattat
    21841 atccgaagta taaggataaa ggctttgaaa ttcttgcgat atcattggat gaagatgttg
    21901 acgactggca aaaagccata aaaaaagata aggtcagctg gctccaggtg atcgataaag
    21961 ggggatggga atccggtacg gtgaaccagt ggcggatcgt tgccatacca acttcttatc
    22021 tgatcaataa ggacggagtc ctggtagcca tggacctgga agggaaaaac ctcgaaaaag
    22081 cattgaatga attgttgggt gactgattaa ttattccaaa tgagatacct gattatttct
    22141 ggattattga ttttattgag cgtgcaaggc aatgcacagg agttatatgt tttcagtgaa
    22201 ccggcatcca atttacctgc ccgttcgctt agtgtaaaga tcaagaatca ttttgtgacc
    22261 cgtgataaca tttatggtcg tttttccaac cgactgatgc cacagttttt tttcggtgtt
    22321 cataaaaatc tgatgctgcg ggccggtatt tcggtcgcaa atatgcatac gccggatacc
    22381 cgctatgaat cgtttaatgt ctatgcaaag tatcgtttag tgtccaatga tgatattcac
    22441 cggcatttcc ggttagcggt ctattcggag ttttcaaaaa caaggattcc ttttcattat
    22501 gatgagatca cgcttatggg cgataagggc ggggtggaag cagggctggt cgccacacaa
    22561 ctctggcata agtttgcact atccggtaca attggccata cacaggtttt acataaatca
    22621 agaaaggata atgtgattta tattcccgaa cggggttacc agtcattgaa ttattccctg
    22681 tctggtggat tgctgatatt gcccaaagaa tattccgatt ataagcaaac caatctgaac
    22741 ctctatctcg aaataatcgg gcagcaatta atggataaaa aggccgggtt cctcgatctg
    22801 gctccggctg tccagctgat tttcgggagt accaccaaat taaacatggg ctatcgtttt
    22861 caacttaatg gttccatgca acggatggct tctaatagct ggctcatcag ccttgaaagg
    22921 acttttttga atgctttaaa gaaaaagcac cctaaataaa tgccgtgcgc aaaaccggac
    22981 tgatcatatt gacattttta tacctcgcgt ttaccgcagg gatcagtgtg caacgccatt
    23041 attgtatgaa ccggatggcc gcccttcgct ttacgactca gtcggaagaa cgttgtggat
    23101 tttgcggcat ggaaaaaaca gagcgggata attcctgttg ccacgatgag caggcggttt
    23161 ataaaatcac cgatgatcag caaacgggtt ttccgacaat ttttattgtc aatgaatttc
    23221 aagtagggtc tccggaacac tatattccgg tgtccggtaa aataattact gcttcggtcc
    23281 ctgtcccggc ttacagccat gggccaccgg gagtatccgc tttcccctta tttattcgtt
    23341 ttggcgtatt tcgtatttaa tgaagaatta tcaagtttct atggccggta tggaccgcca
    23401 tgcacctgat tcattcattc atcaaataca tcatcatgaa aattatcata ctgcttgtat
    23461 tgttctttct gctgtttttc agcagaaaag atatacaggc gcaaacaagt tctgtaataa
    23521 caattaccct gaaagtatcc ggcaactgtg aaatgtgtaa agagcggata gaagcggccg
    23581 ccaaagggaa aggagtaaaa gaagtcagct gggatgcgga aaccaaactg ctgaccctga
    23641 cctatgatac gctgcttacc cgtattgaaa aagtacatat ccggattgct gacgccggcc
    23701 atgatactga gctgaaaaaa gctaaaaacg atacatataa agcattacct gaatgctgtc
    23761 tataccggga ccatagtgaa tcaatgacta ctgattcggg taattccctg atttccttgg
    23821 tacgcggtgt ggtattggaa gaggatgatc ggggaaattt caggcccttg ccccgggcca
    23881 gtatttcctg gctgggaacc ggtgcaggta cactgacaga tagtaccggt gtgtttaaaa
    23941 taagtgttac ccaggaaacc gaccggctgg tgatcagtta caccggttat cggccagata
    24001 ccattgctgt acagccggcc acggacctga aaattattct ggccagtaac cagcagttgg
    24061 gggaagttac cctgaattca aaattaagat ctacctatat ctcttccctg agtacgatcc
    24121 gtacgcaggt catgacggaa aaagaactgt tcaaagcagc ctgctgtaat ctcagtgaaa
    24181 gttttgaaac caatccttcc gttgatgtgt cgtataatga tgcggtaaca ggcagtaaac
    24241 agattcaact gcttggctta agcggcaatt atacccaact tactgtggag aatctgcctg
    24301 gtccgcgggg actggccacg gcccttggac tcaacagcat tccgggtcca tgggtggagt
    24361 cgattcagct gaataaggga gtgggttcag tagtgaatgg ctatgaaagt attgccgggc
    24421 aaatcaatat tgaattaaaa aaaccggaga aagcggaaca gctctacgcc aacgtttacc
    24481 tgaacaatat gggcaagtcc gatatcaacc tgaacctgtc ccggaagttt ggaaagaaat
    24541 ggagtgccgc cctgttattg catgatgatt tcctgtataa taaagtggac tttaataaag
    24601 atggatttcg cgacctgccc accggtaatt tgttctccgt gctgaaccgc tggaaatttg
    24661 agaatggcaa aggcttcatg gctcagtttg gggtgaagtt gatgactgat gaaaaaacag
    24721 gaggggagac cgactttgat ccggcaaagg ataaactgac cactaaccat tatggattgg
    24781 gcatcagtac ccggcgatat gaggcttttt caaaaacagg gtatgttttt ccaggtaaaa
    24841 aatataagag catcgggttg caactgatgg ctttttcgca tgagcaggat tcctattttg
    24901 ggacgcagcc ttacctggcc gcgcaacaga gtttatatgc caacctgatt tatcaatcca
    24961 ttattggaaa caccaatcat aagttccgga ccggcctcag ctggacagcg gaccgatatg
    25021 atgaaacgat cagaaacata acttataagc ggactgaacg ggtgtcggga ggattttttg
    25081 agtatacttt taccccgaat cctaaactgg gcatagtggc gggaatcaga agtgatcata
    25141 ataatctgta tggatggttt ctgaccccgc gtttgcatct gcggtatgaa ccggttaaag
    25201 gaaccagtat acggatcagt gccgggcgtg gtcaacgtac ggcgaatatt tttgccgaga
    25261 acaacagcgt actggtcagt gcaaggacaa tagtgatgcc ggccctcaac acgagtgctt
    25321 atggattaaa tccggaagtg gcgtggaaca aaggcctcag tgtggatcag caattccgtt
    25381 tgtttaaccg gagtgcttcc ctgagtatgg attttttcag aaacgatttc cgcaaccagg
    25441 tggtggtgga cctggaaaag gcgcgtgaag tctcattcta taatctctcc ggacattctt
    25501 attccaacag cctgcaggtt gaactacaca ccattccatt acgaaacctg gaatggaggc
    25561 tggcctatcg ttactttgat gtacatagta cgtatagcgg agtcgcttta caaaagccat
    25621 ttacctcaaa gcaccgcgct ttcatgaacc tggcttatga aataaaagac tggaaactgg
    25681 attatacatt gaacctgaat ggtccgaaac gaataccttc tacccgtgac aatcccggat
    25741 tctatcaacg ggataattat tctccttcat tctggatcat gaatgcgcaa gtcagtaaaa
    25801 cggtggggaa aaagcacccg gttgatctgt acctgggagg ggagaacctg gggaattatt
    25861 tccagcggga tgtgatcatc agcccggatc aaccatttgg cgcctatttc gatgcttccc
    25921 tggtctgggg gcctgtcagc ggacggatga tttacgccgg gcttcgctat aaaatcaatt
    25981 aactacctgg ctgattatca gcagctatct attattgcca tcggttcctc tttttaaggg
    26041 gaactttttt tttgccggta taccaaaaat aatttaaatt cgtataaata ttattttaga
    26101 cgtgtctaaa aaaatattta gttacctgta acacatgaaa caaaaaccac ttttcttact
    26161 ggccatttac tgctggttca tgttgccgtt ggcggcacaa actatccgtg gagaagtcag
    26221 ggatgccctc agcggggaac cgattcccat ggcttcggtt catgccggcg acagcctgaa
    26281 gaccctgacg gataaacaag gccggtttac aatcaataca tcttccaatg tacacatcca
    26341 tgtatcggca atgggatatg aaacagctgc ctttttaaac aaccagaaag attttctgac
    26401 gatcacgctg atgcctcagc aaaagcagat tgaaggggtg gtgattacgg gcaccttaaa
    26461 agcagtgcgg cgatcggaaa gtccggtggc tgtggaagtg tacaccccgc agtttctgaa
    26521 aaaaaatccg gctcccagtc tttttgaatc cctgcaaaat gtaaacgggg taagacccca
    26581 gcttaactgt agtgtttgta ataccgggga cattcatatc aacggattgg aaggtcccta
    26641 tacaatggtg gcgattgatg gaatgccggt gatgagcagt ctcgcctccg tatatggact
    26701 gtttggtatc ccgacgcaac tgattgaccg gattgaaatc gtcaaaggtc ccgcttcggg
    26761 cttatatggt gcggaagcca ttgggggaat gatcaatatt attaccaaat caccggataa
    26821 agcatcaccg gttaccgctc agctgatgac gagcagctgg aaggaacatt cttttgatgc
    26881 cggattgaaa tttaacctgg gtaaaaaaat cagttcttta ctgggtatac attatttcaa
    26941 ttatcagaac aaggtggatc acaatcagga tcagtttacg gatgtggcgc tccagcaccg
    27001 ggtatccctg ttcaataagt ttcaactgac ccgtaaacat aacaggcttg ccagcctggc
    27061 tggccgttat ttttttgaga accgatgggg aggggatacc cgttggacaa aggaattccg
    27121 gggtacggat agtttttatg gcgaaagtat ttatacctcc cgctgggaat tgtttggtca
    27181 gtaccagtta ccggttcagg aaaaattcct gttctccttt tccacaaccg ggcataagca
    27241 ggattcttat tatggacaat taccgtatct cgctacccag cgaaccgggt tcatgcagct
    27301 gacctgggat aaaattgctg gggaaaatca ctcactgctt acagggattt ctgcaagagc
    27361 caatttttat gatgacaact ccacggccac taaggataca ttaagtggcc gaaataaacc
    27421 agatcgcagt gtgctacccg gtctgtttat tcaggatgaa tggaaactgc atgaaaagca
    27481 cttgttatta ctgggactac gtacagacta tcacccaatt cataagtcca tccttacacc
    27541 ccggtttgcc tggaaatgga gtccgggaca tcagcaggta attcgattga atggtggtac
    27601 gggtttccgg gtcgtaaatc tttttaccga ggagcatgcg gctctaaccg gcgcaagggt
    27661 ggtggaaatc agggaagctc ttcgtccgga acgcagttac aatatcaacc tgaattatac
    27721 ccggctgcta aaggggaaca aatactcatt gcaactggat gggtcggcat ggtactcata
    27781 cttcagcaac cagatcattc cggattatga tactgatccc aataaaatca tctataaaaa
    27841 cctggacgga ttttcatttt ccaaagggat gaccctgaat attgattaca gttatggtca
    27901 ccggttcaaa gcgttattag ggatgacctt acagtcaata aagaaaactg aaaaggatgc
    27961 ttccgggaat aagttaacac aagtaccggt gctgactgaa aaatggagtg gtacctggtc
    28021 catcagttat tccattcccg caggaggctg gtccgttgat tataccggaa atatatatgg
    28081 tcccatgcgt ttacccttgc tcggagtcag ggacccacgt cctgcacatt cccctgtctg
    28141 gagtttacaa aatctgcagg ttaccaaaaa actgggccgg gctatcgagt gttttgccgg
    28201 gagcaaaaac ctgctgaact ggactccggc ccgccgggta ccttttctga ttgcacgggc
    28261 acatgatccg tttgataagt tagttgaata tgaaccggat ggctcagtaa gagtctctgc
    28321 cgagaatccc ttcggactga gttttgatcc ggcttatgtt tatgcgccta accagggccg
    28381 cagaatattt gcggggttgc gattcacatt acgctaaatg agcatataaa gaccttgctc
    28441 tttttttgta tctttacgag gtgaaacgtt tcgcaggtat tttactcatc tccgtttata
    28501 tgttctcctt tgcggagttc cataacctgc tgaagatacc cgtcctgatt gaacatttcc
    28561 acgaacatca ccagcaaaac agtaatacca gtttctggtc ctttattaaa ctgcattact
    28621 tcgatgcata cgtcattgac agtgattacc aggaagaccg tcaattgcct ttccgtgata
    28681 cagattgtgg gttggtggta agtacgtcca tatgcgaata cgcaccggtg gtcatggata
    28741 tccagccacc cgcagaaccc gtccgtgagt ttcatcattt taatgacggt catgcccccc
    28801 aattcacttc cttcgatatt ttccaacctc cccgtgtcct ggcataattt cacttttttg
    28861 tatttcattt ttttacactg attttcagtt caagtaatta tttatgctga atcatattat
    28921 ccgcttttcc atccggaata agctgatcat tgggttatta atgctgggat taattggctg
    28981 gggagccata tccttttcaa agttgcctat tgatgctgtt ccggatataa ccaataatca
    29041 ggtccaggta atcaccgtat ctccctccct ggccgctcct gaagtagagc gactgattac
    29101 ttttcctatc gaacaggcca tggccagtgt gccgggtatt gaagagatcc gctctttttc
    29161 ccgttttggt ctgtccctgg tgaccattgt atttgatgat aacaccaatg tatactgggc
    29221 ccgacagcag atttccgaac gactgcaaca ggtacgggca gaaattccgg ccgatgccgg
    29281 caatccattt atgggacccg ttaccactgg tcttggggaa attttccaat atgcggtaaa
    29341 acccaaaaaa ggatttgaat caagatttac ccctgcagaa ctccgtacga tccaggactg
    29401 gatagtacga agacaattat taggtacacc cggcgtggcc gatgtaagca gttttggggg
    29461 taatctcaaa caatatgaag tgtcaatcaa cactgaccgg ctgaagagta tgaatgtaac
    29521 ggtggatgat gttttcaatg cgctccagct gaataaccag aataccggcg gggcctatat
    29581 agaaaaaggc ccgaccgttc tttatatccg aagtgaaggg ctggcaaaaa ctattgaaga
    29641 tatcgggaaa atttttgtcc ggcataccag ttcaggtatc cctgtttata tcaaggatgt
    29701 ggccgatgtt aaaaccggtt cggccgtccg ttacggtgcc atgtgttata atgatgaagg
    29761 agaagtggcc ggagctgtgg tcatgatgct caaaggcagt aattcctccg tggtgatcaa
    29821 agatgtaaag gaaaaaatca cgcagatcga aaaaacgctt ccggaaggtg tggagatcgt
    29881 tcctttcctg gaccgtacaa aaatggtgaa taatgccatt ggtaccgtag agaaaaacct
    29941 gatcgaaggg gcattgatag tgatctttat cctcgtactc ttcctgggca atctgcgggc
    30001 aggactgatc gtggcctctg ttattccctt atctatgatg tttgcggtga tcatgatgaa
    30061 tgctttcggt gtatccggca acctgatgag tctgggtgcc ctggacttcg gtttgattgt
    30121 agacggggcc gtgatcattg tggaagcagt aatgcaccgg ctatatcata gccggcattt
    30181 tgaaacgagc aacagtatca ctcagcaaac catgaatgat gaagtggaaa gctcggcggg
    30241 taaaatgatg aactccgccg tattcggaca aattatcatc ctgatcgttt atctgccgat
    30301 cctggccctt gtaggtattg aaggaaagat gtttaaacca atggcccaga cggtttcctt
    30361 tgccctgatc ggtgctttta ttctttccct gacctatgtg ccgatgatca cttctctttt
    30421 tctcagtaag aaaatgcatc accgggagaa tttttcggat aagatgatga aatggataca
    30481 acgaatgtat gaaccggcat tacacaaagc cctggcattt ccaaagctga tcacgatttc
    30541 tgttttcctg ttttttgtat ttgccgtttt catcctggct cggatgggag gggagtttat
    30601 ccctaaactg gaagagggtg actttgcggt tgatacgcgg ttattgaccg gcagttcgct
    30661 gtcacaaacc attgaatcga ctcagcagtc ggccggaatt ttactgaaac gatttcctga
    30721 agtagagaaa gtcgtcacca agatcggttc cggtgaaata cccactgacc cgatgcccat
    30781 cgaagcgtcg gatatgatgg tgatcctgaa agataaaaat gaatggacgt cggctaaaac
    30841 ctttgatgaa ctggccgata aaatgagcaa ggcgctggaa gatgtccccg gcattaccgc
    30901 cggattccaa tacccggtac aaatgcggtt taatgaactg atgactggtg cccggcagga
    30961 tgtggtctgt aagattttcg gggaagacct cgatacactg gccgcatact caaaaaaact
    31021 cggggatgtg gtgagtacag tggatggggc tgcagacctc tatgtggaga ccgtaactgg
    31081 attgccgcag attgtaatca gttttaaccg ggatcagatg tcgcattacc agctgaatgt
    31141 agccgatgta aaccgggtga tccgtaccgc ttttgccgga gaaagcgctg gcacaatcta
    31201 tgagaatgaa cgacggtttg acctggttgt acggctcaat cagcaatccc gccaggatct
    31261 gacggatgta cagaatctgc ttattgccac tccaatcggt acacagattc ctttgtacca
    31321 ggtggccaac gttgaaataa aagaaggccc caaccagatt cagcgggaag atgctaagcg
    31381 aaggattgtg gttggattta atgtaagagg acgggatgta cagagtgtgg tggatgaact
    31441 cagcaagaaa gtaaatgagc aggtgaaact gccttccggt tattatgtga cttatggcgg
    31501 ccagtttgaa aacctggtgg aagcgaaaaa aagattgagt atcgctttac ccattgcgct
    31561 tttactcata tttatcatgc tctattttgc ttttggtaaa ctcaaatacg ggctgcttat
    31621 tttctccgcg attcctttgt ctgccatcgg tggtatcctg gccttatggc tccgtgggat
    31681 gccgttcagt atttctgcgg gtgttggatt tatcgcgttg ttcggggtgg ccgtgttaaa
    31741 tggaatcgta ttgatcactg agtttaaccg gttgaaagaa caaggcatgc atgaccttaa
    31801 atttattatt atggaaggaa ctaaagtccg gttacgtccg gtattgatga ccgcctcggt
    31861 ggcttcactt ggttttttac caatggcgtt aagtgccgga gcgggtgctg aagtacagcg
    31921 gcccttagct accgtggtta tcggaggcct ggttactgca actttcctga ccctggtttt
    31981 attacccatc ctgtacatgt ggctcgaaag agagaagccc agaaaaagaa aagcgcggcc
    32041 agtagctgtg ctcttcttgc tgttgtcatt gggtaccgta gcacaggcgc agcaacctga
    32101 aagtaaacgg ataatgctgg acgagttgct gaaactggca gacaaacaga atattacggt
    32161 acaggccctt caaaaggaat cggcttactg gaagcaactt caatcagctg tggccgacca
    32221 gggaagaacc ctgctgagtg ccgaatacgg gaagatcaat agtattaata atgacacccg
    32281 gttcaccctg agtcagtcat ttacacttcc agtggtatac aaacggcaac gggcctttta
    32341 cagtgcgggt gaacaatccc agcaggccat ggtacaatgg aaagaacagg aactgcaccg
    32401 ggaaattaaa ttgctgtact ggtcaatgac ggatctgtta ggccgtcagc aattgctgct
    32461 acaactggat tctgtttaca gccgggtact gcaggcggca acgcttcggt taaaagccgg
    32521 ggaaaccaac cagcttgaaa aaaccagtac tgaggcccag gtggcccagt tgaaaatgca
    32581 gctggcacag atacaatccg gcttactgac tgatcagcag caactgcaat ggctactgaa
    32641 cagtaacgag ttctacctgc cggatccggt tagtgtcaac aataaggaaa tggaatggac
    32701 ggacaccagc agtatatcta cccatccctt ggtaaaatac cgggagggcc aggtgaaaat
    32761 ggctacggca cagaccgata tggaaaagaa caaactggtg ccagatctgt ctgcaggata
    32821 taacaatatg tccattgtcg gatatcagtc ccctgatggg gttacccaaa aatactatgg
    32881 cccgggtcag cgttttggta cggtgaatat aaccatgggc gtaccgctgt ttaccaaagc
    32941 agcccgcaat aaagtaaaag cggctaaact gagtgaagaa gccgcacaac tgaatgtgaa
    33001 agccgcggat cagcaattaa agatgcagtt actgcaatac caggaagcgt ttaaaaagca
    33061 acagcaactg gtacaatact acaataatga aggactggca cagtcagcac tgatcattcg
    33121 tcatgccgga ttgaactttg agaaagggca gatcagctat ctcgaatgga caatgctgat
    33181 gaataacgca acgggtatcc aactggccag gctggatgca ctggctcaat taaacaggat
    33241 ccgtacagaa attgaatact taaccggtaa ataaaattaa aatgaaccga attaacattt
    33301 atatattggc catgctgttt acagcagcct gttcctccaa gaaacaggcg gccacggaag
    33361 ataaagaagc ggtattggct gaccaggtac aattgaccga tgtacaactt aaaaatgcgg
    33421 gtatcgaaac cggcaaagcg atccgccagc cgttgaatac agtactcaaa gtaaatggac
    33481 atgtggatgt tccgccgcag aatattgttt ccatcagctt tcccatggga ggttatctgc
    33541 gctccactaa attgttgccg ggtatgcata tcagccgggg tgaagtgatt gcggttattg
    33601 aagatcagtc cctggtacaa ttgcagcagg attacctggt gaccaaagcg cgattgcagt
    33661 ttctgcagca ggaatatgaa cggcagaaga tgctgaatga aaacaatgtg aatgctgcga
    33721 aaacattcca gcaggctgaa tcggaatatc tctcccagaa agttttgctt aaagggtaca
    33781 gtgaaaagct gttcctgatc ggtatcaacc cggccgggct ttctgaaaac agcatttcca
    33841 ggagtgtttc ggtgcattcc ccgatcaatg ggtttgtgtc aaaagtgaat gtgaatattg
    33901 gaaaatatgt tacggccacg gatgtattat ttgaactgat caatccggat gacatgcatg
    33961 ctgcactaac cgtatttgaa aaagacctga ataaagtgag tcccgggcaa aaagtaaaag
    34021 tctctttcgt ggatgatccc gctaccacct acgattgtga agtgatcctg gtaacccgga
    34081 atgtggatga gaaccggggt gccctggtgc attgtcattt tgaaacccaa ccgaagaacc
    34141 tgctgccggg gatgtttttg aatgctacca ttaatatcaa caatgcagat gtattgaccg
    34201 tgccggaaga agcggtggtg cggtttgata ataagcaata tgtttttgta caatccggaa
    34261 aaaacgaatt cagcatggtg gaagtagagc ccggtgtaaa agaaaacgga cggttggaaa
    34321 tcaagtcaaa aatggaagga tttgaacaac agctattggt gacccggaac gcttatgcgg
    34381 ttttatcaaa aatgaaaaac acggcagaag aataattttt tgcctgttta tgatagccgg
    34441 gatgactcgc ggagtggaga aagttgatat tcatttactt ccttccttcc cgtcttcccg
    34501 gctatttttt ttgagaaatg tggataaaaa acaaattttt ttttgctcgg tagtccgtta
    34561 gatttgctcc cccaacatga taacctatgg agctgaaata cagtcaacag atcgccggta
    34621 ccctgagcct caccctgaag caggtcagca gtatttatga cctgcatacc gaaggatcca
    34681 cgatcccttt tatagcccgt taccggaaag aagccaccgg taatctcgat gaagtggtga
    34741 ttgctaatgt gatcgaccag ataaagtatt tcaatgaatt ggagaaacgg aaagagacgg
    34801 tgttgaaaac aatcgaagaa gcgggcaaac tgacccccga gttgaagcgg cggatcgaag
    34861 attgtatcaa tgccactgaa ctggaagata tttacctgcc ttataaaccc aaacggaaaa
    34921 ccaaggcctc ggtggccatt gaaaagggac tggaaccgtt ggccacgcta ttgtttcagc
    34981 aggggccgca taatcccgag gaagaagccg cgaagtatat caacgaacag gtaaaagata
    35041 ctacagaagc cctccagggt gcccgggata tcatggctga atggattgca gagaatgaac
    35101 aggcccgtaa cctggtgcgt aaactcttta cggaagaagc tactgtttcg tctcgcgtgc
    35161 tgactacaaa aaaggaagag gctgatgccc agaaataccg ggattatttt gaatttaaag
    35221 aaccgttggc acaaagtcca tcgcaccgta tactggcaat ccgccgggga gagaaagaag
    35281 gatttctgat catgacgatt aatattgaaa aggaaaatgc ccacgcggaa ttgaaaaggg
    35341 ttttcctgaa agcctccggt gcggccgcca aacaggtgga actcgctatt gaggattctt
    35401 ataaccggtt gttgcaatcc tccattgaaa ctgaattccg catggccagc aaaaccaggg
    35461 ccgatgaaga agccatccgc gtatttgcgg agaatctgcg gcagttatta ctggcgtcac
    35521 cgctgggaca aaaacgcatc atggccattg accccggctt ccgtacgggg tgtaaagttg
    35581 tttgcctgga tgagcatggt actttcctga aatatatcac catcttcccg catgcgccgc
    35641 agaatgactg gaatggggca gtacagacga taaaagaact ggtggcccgt tatgatatcg
    35701 aagccatcgg gtatggtaac ggtacggcca gtaaagaaac agaacagctg gcgcgaggca
    35761 ttgattttgg taaaccggtt tccgtcttca tggtgaacga aagcggggct tccatttatt
    35821 cagcgagtga agtggcccgt gaagaatttc cggatgagga tgtgactgtt cgcggggcca
    35881 tcagtattgg ccgacgttta ctggatccgc tcagtgagct ggtaaaaatt gatgccaaga
    35941 gtatcggggt aggacagtat cagcatgacg taaaccagaa ccgcttaaaa gaggcactgg
    36001 accaggtagt ggagagttgt gtaaactttg tgggagtgga tgtgaatacc gccagtaagc
    36061 acctgttgac ctatgtatcg ggactcagtg ccaccgtggc gaagaatgtg gtggaatacc
    36121 gtaccaagaa cggcggtttt aagagcaggg aggaactgaa aaaagtttcg atgttaggcc
    36181 ccaaggcttt tgagcaatgt gcgggcttct tgcgcattcc caatggggct aacccgcttg
    36241 ataattccgc cgtacacccg gagagttatc atgtagtaga agctatggcg gcaaaagcca
    36301 gcagctccgt acctgaactg attaccaatc cggaagtgcg caagcagatc aaagcccgtg
    36361 aatttgtatc agagaaaatc ggccagttta ctattgaaga tattctcaag gaactggaaa
    36421 agccaggccg tgatccgcgc tcgcccattg aagagtttcg ttttgctgaa ggcgtaagta
    36481 ccatggaaga cctgaaaccg ggcatgaaag taccaggtat cgttaccaat atcaccaatt
    36541 tcggggcctt tgtggatgtg ggggtaaaac aggatggtct ggtgcatatc tctcatttat
    36601 ctaatcgtta tatcagcgat cccaatgaag cggtgaagtt gggacaaaag gtaatggcga
    36661 ctgtgctgga agtggatacc gcgaggaagc ggattgcttt gtcattgaag gatggacagg
    36721 gaagtcagga agtccgcaaa tcggaaagac caggagacag aaggaccgga agtccgaaag
    36781 atggaaagtc cggaggagca aagccgcaac cgctgaatcc ttttcaggct aagttgatgg
    36841 aattgaagaa aaacttcaaa gattagtcgg cagtcggcag tcgggagtcg gcagtccgtt
    36901 cttagaacta ttacttcaag gcacgtcagt cgtacttcgt atttcgtacc tcatacttta
    36961 ttcttcttta aagttttttc aatatagaaa ccccttcttc ccatgggccg aatccggcat
    37021 cagcattgat atgcccggca tcaccgatgt taatgtattc actgccccag caatcggcaa
    37081 aataacgggc tctttcggcg gatacccaca catcattatt gctggccact acaattgttt
    37141 tgaaggggat cttttccagc ggaatcggcg tgaagcctgt ggcaggaaac gtatagaccg
    37201 gtgcttcaat atcactgggg gcaaccagca tcgctccttt aataattttg ccggaatgct
    37261 ttgcccaatg ggctatcgtt gaacagccca ggctatgacc aataagaaca actgtagaag
    37321 gatcaagtgc agctaccgca tggtcgattg ctgtcagcca atcggataaa gcgggtgcat
    37381 cccattcctt ttgttcaatt cttttgcaat ccggaacatt tttttcaaac caggtttgcc
    37441 agtgggccgg acctgaattc ccgagtcccg gtatgatcag gtagtgggtc atcggtaatc
    37501 ttctcttacc gggttgaata catcgatcac tttacaatca gttacagcca ctgctccatg
    37561 ccatacgttg gaagggatta catagtacat gccggcggtg agtggacaaa gctttccatc
    37621 aatggtcatg tccagttgtc cttcgagaat ataagtgatc tgttcatgcg gatgctggtg
    37681 ctgagggaga tcacttcctt tttttatttc cactaatcca agtgtcatat tggtgccatg
    37741 cgcataatag ccggttagtc cgggcgcaag gtttttcgga gtattgtttt ttagttgttt
    37801 catcaagtaa tcattttaca aattcaggac tgttaggtcc cccttcaggg ggatttaagg
    37861 ggcaaacagg cagaaagacc ctcccaccca ttctttgtct ttgttatacc gtacaccgac
    37921 cattttccct ttgctcagtc tcgccaggtt attggtgggc ataggtcttg tactgccgtc
    37981 tttccagaaa tagaagatcc ggatctctgc tttggcaggg atatcggggg tgacaatcac
    38041 atccgcgtat tttacttttt tctggaggat ccagttctcc ggatccttga cggcttcaat
    38101 atcggcagcg gttacatcaa tgatcacgcc ctgaccggcg aaggagaata acggtttcag
    38161 tacatagttc tccaggtcgg ccggtaattg tttgatctca ttgaggaaga aagtgggcgg
    38221 cacatacgga tgacggatca ggggtaaagt atacttgctg atccggtaaa accagttggg
    38281 atgcggacac cattccacat ccagctcttc aagcaggagc tttccttttt cctgtacttc
    38341 cggactttgt tgttgcaggt cgtcaaaaat gatgcggtta taaattcgtt taatttccgt
    38401 tttcttccca ttattgaagt agtatagttt cttcccttct ttaatcagtt cggtcagaca
    38461 gaccattttg atgccggtat attcttcggt acagtagaaa tcgatccttg ttttttgttt
    38521 gcggggaaag atctccagca ggatcacatt ttccgggtta tgattaccca cgatgatctc
    38581 tttcagtaat tgcagatagg tttcgcgggt atgtccgccc aggtaactac tgtatccttc
    38641 cggtatcggg aaatgacgac tgtatacatc atccagtaat acttcatatc caaacagggt
    38701 ggggaagccc tgcatctcga ttagttgcgg ttcgtattca ccttgttcat tgacacaaat
    38761 gccaaaatca aaagcgataa aatgagaatg atcattttct cccggcactt ttactttttc
    38821 cggaatggca ttacgggtca gctcattaaa tttcggatcg gagatgatgt caacgatatt
    38881 ctcacaggca tccagcactt tgcgggtgaa gtcagccgga atgaagaccg gtgtttcagc
    38941 aactcggaaa tcgatctgtc ccggccatgc actatgcaga tcttcgagga aggcctggta
    39001 tttttcttgc gtaaaagctt tgttatatgc ttcccggatg gatggcacca tagtcgttta
    39061 tttacatatg gaagttaatg attgcggacg gggcagcaaa attaaacagg ggctttctat
    39121 gcaggtactt ccagcaattg acccactgcg tgattcagga aattgggcag tatgtgggag
    39181 tatgtccaca cgattttatc cggatcgtcc aggtgttcca gcatgttctg ccatttttcc
    39241 tccccatggt tttcaatgac tgtggttttt ttgtcttccc gttgcaggta catactcata
    39301 cctgatgcat cggcttccgg atgaaactgg gtgccgatca tataatcatt aaagcggata
    39361 cccatgatgg ctctttcata cggtacatgc gggcggcttt tctcaataca aagaattgag
    39421 gcccccattt cattgagcag gctatgatgc ggctgaatga cctggtaatc gcggctatct
    39481 actgcataaa acggatcttt taatccatca aacacacgtt cctccttacc ggagtgtaac
    39541 atatgaatcg gaaatacgcc aaaagaagtg gacttacgtt tacagaccac gccggcatta
    39601 aagaagcggc ttgcgagctg gaaagaatga cagataaaaa agatatgttt ctttacggag
    39661 ttgtcgggat tttcgttcca ccggtgcatt ttatcgagcc agcggcacca ggcaatatcc
    39721 cagtcatcat agcggctggt cagcgggtca ccaggacctc cactcgaaat atacagatca
    39781 tatgaagtat cgggaagttc atttttcagg cggacatcat attcctttgc ataaaggagc
    39841 agatcattct tctcagccca ttcgatgagg atgttacgga tacaacgcat gccctggttg
    39901 gcggcgcctt catagaggtc gaggacggct actttcacca cccgtttttc ggtccagctc
    39961 atgggggcaa aaatacgatt tttaagtgga agcaggaagc aaacggttgc gggtttgtcc
    40021 acaggggtgg atagtttttt tgtagtggaa agttgacaat gtacaaggtt tgaaaggttt
    40081 gaaaggttta aaaggtttaa gaggttcaag aggtttaaaa ggttgaagaa gtgttactaa
    40141 aatcgcaggt ctctttaacc tcttgaactt tttaaacttc ttaaacttct taaacttttt
    40201 aaacctcttt aaccttactt cctctcgtaa acccgttctc ctcccacata ggtcatcttc
    40261 acttttaccg ccagtaattt ctccggcact tctttcagca gatcggcatc caggataacg
    40321 aagtcggcaa gtttcccctt ctccaggctt cccttttcgt gctcttcaaa attggagcgg
    40381 gcggcccaga tggtcatacc gcgtagtgcc tgttcacggg ttaatgcatt ttccatctga
    40441 tagccggttt ccggccatcc ctttgcatct tttcggatca ctgcagcgta aaatgtttta
    40501 aaggtggaaa tatcttccac tggaaaatcg gtgccaagcg gcagccatcc gttttgttgt
    40561 aataactgct ggtaagcata agctcctttc accctttccg ttccgagacg atcaccggcc
    40621 caatacatat cactggtggc atgggtgggt tgtatggagg gaatgatgga gtattgaccg
    40681 aatattgcaa aatcgttttg attcaccacc tgggcatgtt cgatgcgcca gcgcagatca
    40741 tttttgcctt tcaggtattt ggcataggta ttcagtatgg tccggttacc actatcgcca
    40801 atcgcatgcg tacacatctg ccagcgttgt tcactcagcc agctggccac cgaatcaaaa
    40861 tgggcgggct gacttaacag gaacccataa tggcccggtt tatcctgata aggattcagc
    40921 agacaggccc ccctggatcc aagggcgccg tcgccgtata ctttaaaact acggacattc
    40981 aaccggtctg ttttgatagg gccgtttttc ttcgcgtact cgtaattggc tttttcatca
    41041 ctcagcatca catacaaccg cattttaagc tcaccggccg tttgcagttc ttttatctgt
    41101 tctaccgagg tataactcag gccgcagtca tcaatcgtgg tcaggcccat ggcaaaacaa
    41161 tttttctggg cagcctgtaa tgctttttta aattgttccg ctgtgctggc cggaattttt
    41221 gaactgacaa ggtctacggc attgtcgatc agtatgccgg tcaggctgcc ttccatttct
    41281 tccacctcac cacccgttaa agtatcacct gcttttattc cggcaagttc aagggctatc
    41341 tgattggcca ctgcggcatg tccgtcgatg cgtgccagca ataccggtct gtcggggaac
    41401 agtgtatcca gtgattcatt attgggaaat tctttcatgt cccagtcatt ctgatcccag
    41461 ccccgcccgg taatccatcc ctctttattg gtggcagcaa atgatttaac ccgttccagg
    41521 cattcctccc agcttttagt accactgagg tctacggtct gcagactcag tccatatccg
    41581 acaaaatgag catgggcgtc aataaaaccg ggataaataa attttccgcc ggcatccagt
    41641 ttttcaacgg cttcgaattc tttttccaga tcggccactt taccagtggc gagaatcttt
    41701 ccttctttga ccaccatggc ttcagcggtg gagaaagcag tgtctaccgt ataaatggta
    41761 gcgttgaata ccaggaggtc cgcttgctgt tttgaggtgc aggcaaaaag aag
//
LOCUS       DAOPMW010000002        36062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_982_length_36062_cov_8.597189, whole genome shotgun sequence.
ACCESSION   DAOPMW010000002 DAOPMW010000000
VERSION     DAOPMW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 36062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36062
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_36062_cov_8.597189"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLU11_00175"
     CDS             <1..359
                     /locus_tag="PLU11_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57545.1"
                     /translation="SWAPPAASRSARSDAMGMGISYITGKRCLYYGTGRSSDVFSVEL
                     DSKGRFSGKPEKVFSIAGMGPSGDDKVRRIRTDNMGNLFVYGMSFNYNLIPQREKKET
                     VYSFLYDAGSESWISK"
     gene            476..6649
                     /locus_tag="PLU11_00180"
     CDS             476..6649
                     /locus_tag="PLU11_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HPN57546.1"
                     /translation="MNKTNYLLLLVASVFLHQVVSAQVSGIVFRDYNGNGTRQAATAY
                     TEPLVQGVVVRAYNAAEVLLASATTSSVGAYSFTAGQIPSGTAIRLEFTIPSSGSCLV
                     NSAFDFASGSGATHGTAVRFLTAGAGAVNINFAINDPNDYLSTAAPFTAAFLFTNRYH
                     SGNATGSGTSAAGGVFYKMSYTVKGQTTLPASGLLATNAQIGTCYGIAYSKQADRVFT
                     SAFLKRHNGLGPANGTFNNAPGAIYMINPNLTAGTGAASYFSSLDALGYPTHNSTGSP
                     AYGAGTSYTLTGTGYGATVSYPANGLGVIGTNVNRGLPNDSTTRSNDPAAFGQVGKVS
                     LGDMEISDDGQFLFVTNFYDRKLYQLQLNNVKNPTSATVVNSWSLPNPPLRSTSGIPS
                     AAATYTGASDGTNFYDGTRGLQRPFALKYSRGKLYIGSVTTGEGTGAVSTQDNNTGNP
                     EYTDLWAYVWEFNPTSGTFTSTPVLQFPLNFNRGTNGDGLSETWRPWTNTLPSPWTGT
                     APGFSQYQQPMFSDIEFDSDGTMILGFRDRFGDQSGYDQSGLNGTVRFAGQAMGDMYR
                     AYYNSSSCVFELEQNAKEGPSSPKVATAGANNGDGPSNYTTNNGEFYYRDEVYNANTG
                     ATVGTFHLNTVSGALAIIKGTDSVVVTTMDPLRAWSSGISWFGSAGGDNGRDYEMFAG
                     AGAGTFPAPAVGATGKANGLGDIELLSAVAPTELGNRLWNDANGDGIQNAGESGLANV
                     TLELYSNGVDGLPGTGDDQLLGTTTTNGSGEWYFNTGNVTDGDPTTGGNQAGPQPGIP
                     YNIRVGSADWTGGAGTGDLANYRLSVTDKLCAGAADLSDNDASLSSGNIPMISLTIAA
                     AGQNNHDLDFGFTPLARVGDKVWRDDDQDGVQDTGEPGVAGITVTLYASDGTTVLATT
                     TTDAYGSYYFDQLAAGNYIIGVTLPVNYQFTSSTGTSEADGTNSDVSSSTGKTTAITL
                     SAAENQQQIDAGIIYTPSLTNSIGDRIWLDLDGNGSQNGTEPGLSGVTITLYASDGVT
                     VIATTVSDANGQFNFTGLPASTDYVLGITQPAGLLLTSSSGTTGANNTVNSDFSSSTG
                     KTGLVSSGTAGSQVKGIDAGFVTQPAASAVIGDRVWLDIPGGTAGVQDATEPGIAGVT
                     VNLYRDSNGDGSISGAEAATPFSTTVSDAYGHYIFNNLTAAVSTGSLYQVEFVLPAGY
                     TRTTADQGADDAVDSDADATTGRTIAYRLLPGQRNLSLDAGLVLSSPAGTSRIGDKVW
                     FDADADGVQDATEPGIAGVTVTLYNSSGTAVASTTTDANGNYQFVQLAAGSYSVGFSN
                     LPAGYSFIPIWSTDDANATNSDVNPGTGRTALISLSAGENETDVDAGLYAGIAAGLGS
                     IGNKVWYDITGGTSGVQDAGETGVPGVTVSLLDAGADGIVGNGDDGSTRSTITNAKGE
                     YIFTDLPAGNYAVQFSTLPASYTATTQNAGADDTRDSDGGAIGTGGAPAGASRTAVLS
                     LTEGEDNMSVDLGLVPPAGTNSLGNLVWFDANNDGLQAGEQGMTGILVTLYNGSGTAV
                     ATTVTNENGEYLFSGLADGSYSVGFTNLPAGFDFSTPSATNDATGSDANRATGRTTTV
                     TLNAGNRNDRTLDAGLISTRAVIGNYVWLDTDGNGIQDNDGSEPPVPGVTVTVYRPGF
                     GPDGIAGNSDDALPVASMITDQNGEYRFGNLLPGDYQLEFGTLPAGLVFTANDAGGDD
                     ALDSDANPATGLTGTITLSAGETDLSADAGLFKPRAVIGNYIWSDSNGNGIQDPDERG
                     FAGVLITLFNNLGVAVAATITDAQGWYQFPNVAPGTYSMQFTNLPAAVTFTTADAGGD
                     DAADSDVSGATIPGITVSTTTTNLTFDGGLLNAVTLPVFIQAFTAVPAGQTVQLKWKV
                     SEEQLVAAYIVEYSSDASNWTVLGSVAATGAEQYSYLHLSPIKGANFYRIRVVDADAS
                     YKFTEVRSVYLQKGNSLLVYPNPSVNGLVYVRLPEQIVGKGVTITVYALNGQQVTRVY
                     LAAAGQTEQLKLNGLANGQYILQLKAPAAGYSETSRLEVMK"
     gene            7124..8446
                     /locus_tag="PLU11_00185"
     CDS             7124..8446
                     /locus_tag="PLU11_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57547.1"
                     /translation="MKTTLHINRAIRQFLVLTVISAATATASAQTSQAIGEMTFENPA
                     LEPTSVATGTANGAEYRFSNVTPQMDAIMTITGRSSSQVVINNLDLTGNGFNKAFQPQ
                     IRYNNGNINSAGSWWIEFQFQFVNKGTKLPAQMSEFHVTGLDIDGNGSRLREWDAFYG
                     GASYTLENNSQLTVANLLGTLNLPLLNGKQFLGSQSDRNNIDTAATDVMTTVKYTNTS
                     TMVIRLGVTTTGSASNANRMYSVWFKNFAYIAPISTLPVKLTAFNASLAKDKVNLKWE
                     TASEINVSHFELERSTDGVNFSQAAMVFSYGAADTKANYAYSDNVASFQVPVVYYRLR
                     SVDIDGKSEISETRIIRLAKDQANSISLLTYPNPVMNELRVTVPNNWQNKQTVFELFN
                     ANGQIASRKATTGCNQTETINTSNLAPGFYIVRVSCDGQTAQQKIVKH"
     gene            8565..10823
                     /locus_tag="PLU11_00190"
     CDS             8565..10823
                     /locus_tag="PLU11_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57548.1"
                     /translation="MRRGNLNLLFTLVLVITGLPVLSQVTIKGVIKDNKNNPVAGASI
                     SIKDSYDGTTSDSAGRYSFKTTERGQQVLQVTSIGYKTAEQSIKLEGATLTVDVVIRQ
                     EISELTAVVLSAGTFEASDRKRAAAVLDPIDIVTTASANGDITQALKTLPGAQQVGES
                     EGLFVRGGTAAETKTFIDGTLVNNFFFSSVPNIAQFGRFSPFIFKGTVFSTGGYSALY
                     GQALSSALILESIDLPERTSANLGLSVIGGSAGYQHLSKNNKSSFGASYSYTNLAPAF
                     AVIKQRQDYSTVPNYHNADANFRIKTSKTGFLKYYGYFSSNKLAFTVNSIDSLGYRDE
                     FAISNTNHFHNLSWRENLPKKWRINAGLSYTNNQDDIRSAMQNNAGNEVQLSGLEAKT
                     FQLDNKGNYFNAKLVLEKRFKGLSGIRFGSEYNFSKDKSVYTAFNNQNYPGGLRESIS
                     SLFAESDVYVTNDLALKGGLRMEHSQYLGKFNLAPRASLAYKLGKGSQASLAYGVFYQ
                     NPERRYLPSPNDLSFMKAAHYILQYQKVLNQQSFRAEVFYKKYHNLIKTDFVGFTEAA
                     VSTNGFGDAKGIEFFWRDKKTIKNFDYWITYSFLDTKRDFLNFPFAITPNFAAKHNGS
                     IVTKKFVQKIKTQFNMSYTYSSGRPYYNISYEGNAYKFTDRGTIPDFHNVSFALNYLP
                     TIGKKDAKSFAVYVLQVSNIFNIKQTYGYQYSYNGMRKEPILPTSRMFVYIGAFISFG
                     IDRTNEVIDNGL"
     gene            10856..11503
                     /locus_tag="PLU11_00195"
     CDS             10856..11503
                     /locus_tag="PLU11_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57549.1"
                     /translation="MKKLLFLALTALFLQPVFAQSEKFTGAMKKNIAEIDSIFKSNEM
                     NRLNDIAATFERIGDAEKTQWLPYYYAAYCQVTLAYVKNEVANNDIIADKVDQLIAKA
                     EALQPKNSELSLLKAMNTSLRMLVNPMQRWQQYGPKVQQYTQDAMTQDPTNPRPHYFS
                     GVSLKNTPEQFGGGCGTAKPIFDKALELYAAFKPASDIHPNWGLQVCQREAEGCK"
     gene            11527..12201
                     /locus_tag="PLU11_00200"
     CDS             11527..12201
                     /locus_tag="PLU11_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57550.1"
                     /translation="MKEAEKPMTEQESLALITSMINRAKDVNHSTGLTSIMWGLVIMI
                     CSIVKLAEIHFEFRLPFDIYILTVIAIAPTIYFSIKQKKESRVTGYKDSFIDNTWLAF
                     GISIFLLSFIMNVLFAELRPMKDSVELATGKNLLYILYEYMGSFFLMLYGLPTFVTGV
                     SMKFKPMILGGIICWISCLAAVYTPVKIDLILTAISALFAWFIPGVILEKDYRKAKKE
                     LGAQHV"
     gene            12194..12487
                     /locus_tag="PLU11_00205"
     CDS             12194..12487
                     /locus_tag="PLU11_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPN57551.1"
                     /translation="MFKDLNPILHSQLRLAVMSLLISVKEAEFTFIKEKTNATAGNLS
                     VQIQKLKEAGYIEVVKQFRDNYPQTLCHITPAGIAAFEEYVASLQGYLGGRNE"
     gene            complement(12667..12810)
                     /locus_tag="PLU11_00210"
     CDS             complement(12667..12810)
                     /locus_tag="PLU11_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57552.1"
                     /translation="MKQKINNALKEQTDRLKKAVERMLNIPAKKAPAYVLQPVRNQHR
                     FPR"
     gene            complement(12843..13232)
                     /locus_tag="PLU11_00215"
     CDS             complement(12843..13232)
                     /locus_tag="PLU11_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57553.1"
                     /translation="MSIKFLSVFALFLQLSTAAQDQSLHVSPFTSTTTVATVPAIVEL
                     KAVVNNQKVQLNWKTEENQQVNLFEVEKSTDGKNFSMAALVFSTDKTDAEEYFFFEKN
                     TGKKTVYRVKIISKDKQVYYSAPVTAG"
     gene            13524..14432
                     /locus_tag="PLU11_00220"
     CDS             13524..14432
                     /locus_tag="PLU11_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN57554.1"
                     /translation="MSDVIRVKDLSKHFGELKAVQQLSFSVGEGQVYGFLGQNGAGKS
                     TTIRMLMTLIEPTGGEIEIFGMNLRKHRKEILRQTGAIIEKPDLYKYLTAKENVRIFS
                     RLSGVKASEKQIMDQLSLVGLAGRADSKVKTYSQGMKQRLGLACALVHNPGLIILDEP
                     TNGLDPQGIADMRQLILHLSRDLHKTLLVSSHLLSEMEMVADHMLIIHKGEKVVEGKV
                     SELLDPEKTMVELLTPDINSAIAEFKSGPWQRHLKEPVKGTVRLECNRNEIPALNKYL
                     VEKGIPVQSLRPLNSLEDYFLSLTKV"
     gene            14433..15317
                     /locus_tag="PLU11_00225"
     CDS             14433..15317
                     /locus_tag="PLU11_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPN57555.1"
                     /translation="MFIELLKIELYKIFKRPRTFIAFGAIAVIILLVQFALKVNGKDF
                     VEFYTDSQADTFDIPFDKILNGYFVCVAVLHMILIHVPLLVALIAGDMISGEANLGTL
                     RLLAGKPVTRTQLIMAKFWASTIYVILLLICMAFFALLGSMIIFGTEDLVVFKETDMH
                     IMKSGDVIWRFFAAFVFAALALTTIAAMALMFSVFSENSLGPIVATVCVVIVFTIVQQ
                     LQVPVFEQTINPWSFTTHMLGWKGFFYVEKNAEGATIPGSIENPAALVRSGLILLGYI
                     VLFLTISIRGFNKKDILS"
     gene            15327..16019
                     /locus_tag="PLU11_00230"
     CDS             15327..16019
                     /locus_tag="PLU11_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57556.1"
                     /translation="MKQRLLFFFAFSLFCTQLPAQAAKELLQKVKAKYDKVNDYTATG
                     KMKTNVIFIKAPIANIKVYYKKPDKLKIKNENGVSFIPKGSANVNMNNVLGLSNFEAV
                     ESGTEKVAGINCKVIKIFPLSDEENITRATLYIDEARLLMIKSVISTRENGTYELLMT
                     YKKYAEWGLPDKVELSFNTRDYKLPKGVTIDYDNGTGKGQPRDTKNQKGKVEIDYSSY
                     EINKGVPDSAFN"
     gene            complement(16022..17104)
                     /gene="pdxA"
                     /locus_tag="PLU11_00235"
     CDS             complement(16022..17104)
                     /gene="pdxA"
                     /locus_tag="PLU11_00235"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPN57557.1"
                     /translation="MNTNKPVIGISCGDVNGIGTELIIKTFSDNRILEHCTPVIFASN
                     KLINFYRKTMPELNLNYSSTKEFNRINHKQVNLFNCWEEEIQVTPGQLTETGGKYAIL
                     SLQTAVAALKQKHIDGLVTAPIHKKNIQSAEFNFTGHTPYLKEMFGVKDVVMMLCAGD
                     FRVALVTEHVPVGEVAQYITKEKILSKLALINQSLQKDFGIEKPKIAVLGLNPHAGDE
                     GLIGKEEESIIKPAIKEAKNNNMLVVGPYSADAFFARRSFERFDAVLAMYHDQGLIPF
                     KTLATGEGVNFTAGLPAVRTSPDHGVAFDIAGKDKADPSSFMTAIFECIDIINRRNGY
                     HESRKNPLRKMTPSILANAKDEIIEE"
     gene            17373..20018
                     /locus_tag="PLU11_00240"
     CDS             17373..20018
                     /locus_tag="PLU11_00240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPN57558.1"
                     /translation="MKAIPFYIKSLSLCLLFFLLHEIAGAQLTVIPYGSSWKYWANSQ
                     ANYPANWHTTGFDDSAWPSGTGELGYGDGDESTCIPAASPVAGTLCTPTGNKWPAYYF
                     RKVINIPDPAIYTNFSFNIDRDDGFVVYVNGVEVSRNNIAAGAVSYGTSATTAVEDGI
                     ISFTVATSAFVSGNNTIAVEMHQASVSGSPPVSTSSDISFNLQLLANDAFSAGLSRGP
                     YLQSGSQDGITIRWRTATAQNSKVEVGTSYGSYPIVVNDASSVTEHVVRVTGLSTDTK
                     YYYRIGNSTDSQLPDANQFFTTLPAFNTTRKIRVIAFGDCGRGNVAYQDESLASYLDY
                     LTTNSIDAADAWLLLGDNAYNSGTDAEFTANFFNIYGSNMLKNHKLYPAPGNHDYGNS
                     AANKALRNMAYHNSFTVPQSGESGGVASNKQNFYSFDIGNIHFLSLDSYGTESDGTSI
                     ETAGGSALKTWLTNDLAANTKKWTIAYWHHPPYTKGSHNSDSESDLVNIRQNFISFLE
                     TRGVDMIVCGHSHNYERGYLIKNYTGSWASFNAGTHAVSNSSATYTSSATCPYVYNSA
                     PANHGTVYVVSGSAGASGTMNTGFGTGPMPYALNDGGVFYFEVEDNRLDAKMLRRNGT
                     IFDQFTIIKDVNKTTNYTISNGSSKTLTASWPGNYSWSTAATTRSISVTPPINAVTNY
                     TVEDEYGCITDQFNVTTSSTLPVLLKTFDVRRMDAVVQLNWTIVADQTAAQFLIERSL
                     NGRDFVILGTVDVFPGSGEQQYSYTDHQPVPGTVFYRLSATGAGTAQTYFGIRKLNGN
                     THTGFDLEIRTGVTSSIVLDCSTATDDQYSLTIYTVNGVKVSQTDLSLQKGTTRKVIE
                     LKSGWYICELRNSRGEAVQQKAVVQ"
     gene            20025..24329
                     /locus_tag="PLU11_00245"
     CDS             20025..24329
                     /locus_tag="PLU11_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57559.1"
                     /translation="MKFKLIILFLLTGFISTRINAQVLRTFTPRYSNSSVNGNITFVA
                     NNIITSSGVSTTEAPPGGSAVNNGNTGANINIDADTLIPFGSSWKYYSAGAAPAGSWK
                     DLGYNDAAWSSGNGELGYGDGDETTCIPSGGGGTLCLPTGNKYLTSYFRKTINIPSPT
                     AYTSYQFSVERDDGYVVYVNGVEVARNNMPAGAVAYNTLASSAIEDAVITFSIANTYF
                     ATGNNTIAVEIHQANASSSDLSFNLQLACTDGVIFNSSSADLSLPSCSQVLFAGLYWG
                     ATQGTDGINVSWITNETNVKLKLPGSSTYTDLVSTQTDYHNNTLVPGLPHTGYRCFVD
                     ITALMNTTSPDGTYTVANVCSPAGIVNAAGGWTIVIAYSDPSTILRNLTIFDGSAIMN
                     GGDPALHIPIDGFLTPPSGPVSCELGAVVFDGDRVSTDEYSFKQNSNPLVGVYTSLTP
                     NATANLNDMWNSTISKYGAVVTTRSPAHNNTLGYDADILIVPNTLNAVLGNSQTSASI
                     RFSSPSENYMLQVATTAISQYTPSFNLVKSLTDVNGGSLVPGDVIRYRVDYHNNGNDA
                     SNQSVIYDNIPTGTTYVPGSLVINGVSKTDALSDDQAEYDFTNNRVLFRLGTGANGST
                     GGALAIGASGYIEFDVYTPSSCLLVSCSSTISNSARINYTGALSGISLYDSSGVDVSG
                     CTVLGPVTATLTGSCMPLGDTILTNSCPASSVTLPAGRYGGYRFYTGMPFGAGTLYDP
                     AVAITTTRVIYAWYDGPGSCDDTLQLNVYILACPDIDDDNDGLPDYLELNNPVALQDH
                     DSDGTPNWNDAQYPGFTDNNADGFNDNFDPSADSDNDGILNFYDPDFSGYVDSNGDGV
                     NDNMDKDLDGIPNHLDLDSDNDGIPDTVESFGVDANGDGRIDNYTDTDNDGFSQNVDA
                     NNTGVSGSSTGLGALDTDGDGLPNYLDLDSDNDGIPDIIEVYGADASNSAKVSVFTDT
                     DGDGITDSYDGDVGNDTVAENAAAALLRTGTDADNNGRADSWPYKNMDNDSKPNPYDL
                     DSDGDGITDVKETQLTDANWDGRVDGAVNADGRNTVLAALGSMVLANRDATGRADLYD
                     IDSDDDGIPDNVEGLTTAGYLLPAATDSDADGIDDRYDNYVGFGGDGIHPCDIDGDGT
                     PDYLDSDTDGDGLIDIIEGNDLNLNGIVDDLVTLTGVDTDGDGLDDRFDNNNSNAEST
                     SAYMGDGGTTSGDPTPGSITTVQHTIIAYGCASERDWRCIPYLLNCEYTRFEANADGQ
                     YVRVDWTVRCSQQPAYFIVERSADRQHFDSVQRVEARSVIHEEEAYQVIDRSLNTQLP
                     VLYYRLKTVLTSGKVNYSAVIPVLQVNKPNDSISLFPNPVRSSMTVGISVSSNARAVM
                     DIIDLNGRIHFRASQVLVKGNTTIELNHAVNLPEGMYIFRVQYNGMILTRKFNKLQ"
     gene            24635..27241
                     /locus_tag="PLU11_00250"
     CDS             24635..27241
                     /locus_tag="PLU11_00250"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPN57560.1"
                     /translation="MKKLYHRLLAAAFICSSFTSQVSAQCTNANVNWDNLEYFITNGN
                     TNYTTFINTPTLFANLVQSQRFAIGTNAVTISSNYPANGILGDNTSHTGETGSFGSGA
                     DINFTGNGTITLTFDTAVSNVQFSIFDLDLSQNVTVTAFEGVTPRVPVLTKPVGGTVT
                     IVGNIGTGPAGGIASSSSNATLNVAVAGPVTSITLVFAGTAGDFWISDIQACVYRNFA
                     TNYYVIAQPFTGQPSYVMVVHDLNTVFYVNPANGRAVSIFTDPDPRVREINNIAYDPY
                     KRIIYYSVDGLERCTPAGSPDSIRHIKKYDMNTETISTLISDVKTAPFNIPTFHAGLE
                     SGGAAFYDADLYVGVEGYKLSGTKNSGRETVIWRIEFASDSITPSKACQVWGTPVDNG
                     SNNIHDYADFGLKDGVIYDFNSSTAVGSGRGQYWQINMQSNTTTSTVVPNILANRPAQ
                     TAQSWNGTIYWVADSIGVFNGTVANTVTGKSRILSSPQSVTWVGPAGDAGEAFRPKAD
                     FGDAPSTYDPVALSPALHEKDTALFLGNVFTNDWDWEWDKQTSSDASGDGVDDNDGLT
                     YVSVFDKTTGNYLVQANIYNNTGADATLIAWFDYNGNGVYDASEALAPITIASSPVVQ
                     QHYLHWSGISSTIANGTYTYLRLRLTSSSNSMTTSNPTGYFNNGEVEDYRVLVDNFPL
                     SVNLLSFDAKATGRRTAKLSWKTINESELSGFGIERSADGVNWTNIGFADAKGNNNTG
                     SNTYEFIDQQALKGQSYYRLKLTDINPVTSRFSETRAIRIEDVVQNLTLVPNPAQAST
                     SMHLSSTANIAATIKVMDMTGRAMQTIKTALNPGENVIQLNGLEKYPKGTYLIQVVAE
                     DLVINRKLVITK"
     gene            27323..30379
                     /locus_tag="PLU11_00255"
     CDS             27323..30379
                     /locus_tag="PLU11_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57561.1"
                     /translation="MKNRLLSLALILATMQFTSVCLTSCGLRDERYFETEERENEEEE
                     ENEEEEQNGADKQLSAWFQSKGYPDASNLTQKYAAAWEEFLELKKRSPFQQYATRLQG
                     MGSWSYVGPKAFGGRILCLAINPQVNAAGQRTIFAGSAGGGIWKTYVSGIGASAWQPV
                     ITNTAVLGVSSMVYHPTDTSIILAGTGESYRIENYSSSPYSSNQVSNIGRNAWKARGT
                     YGVGILRSADAGLTWTQVLIKSQSDLFGIQKIKYHPTITGTVFACATDGLYKSTDSGV
                     TWSLIWSGTYCSDIVINPSNVNQIVIAAGNVTNSPKGVWRSTDGGTTFTQVTAGGFPT
                     AAQFKGSTNFTILGTNTIIASVGVGDIDNNGTYNEREVYRSDDFGVNWASVSNSTHAK
                     WQSWFAHGVTAFPGSTNKIFMFGVKKHVLVMSGTPATTGTLTNIATGAASSSYLVDGS
                     QEGSTTYVHDDVHDVQFVPGSTTTAYWATDGGVYRTTNANASPITGMTFVSCNGGLYA
                     QQFYPTIAQSQTTANLAIGGLQDNGVIRYNGTGWARVIGGDGTTVSFKPDNELIVLGS
                     NDTRGVNRSTNGGTSFPTSVMSYLGNIPNGDDDRTAFCAPVVVAPSNTNRWYVGSDNI
                     HVSNDAGVTFSGDVAGVPGTSYIEAFHKPAIAIGVSYSNADVLYVSVSPFAQNLLTDG
                     LYYNPPATIRKSTNGGGGFTTVTTGLPDRVVTDFAVSPTNHDSVFVTLSGFGTTHIYV
                     TGNGGSTWSPRGSGLPDVPFNTILIDPANPQILYAGCDFGVYVSADRGANWYDFNNNL
                     WDATLVMDLAITPGNKLRAATHGKGVFETARWDGMVTLPVNFISFEGANAGKNNNLKW
                     VVDQESNLSRYEVQRSTDGFSFAAVKQVTARNSAIQTTYSILDPLGQNPAPVYYYRIR
                     SIDQDGSYTYSEIVAIRINDGQKFEVLGNPFSQSITIRYTTSRTGKLSIALTDLQGRI
                     VRREELIAATGTGTYSINNLTGIPAGVYVARFDMEGKSIAIKVIKQ"
     gene            30577..30990
                     /locus_tag="PLU11_00260"
     CDS             30577..30990
                     /locus_tag="PLU11_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57562.1"
                     /translation="MKHLNACLLLLVIVMASSFNNISSDVTRSKNGYNANTVFAGFDF
                     FRTHRQGKDGITATWGYSGGTITGYIVERTYEDATDPYASWETVAIVAHTGARSYKVT
                     NTNVTPGFISYRVRVMNGNTEIAVSPVSTEHIVAH"
     gene            31045..33006
                     /locus_tag="PLU11_00265"
     CDS             31045..33006
                     /locus_tag="PLU11_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57563.1"
                     /translation="MKTPLRILCFLIFTCNLSQAQITYPIIKANFGVEADLRSNFFNG
                     LVQSGNDDWFLFPGTPGAGQFVIDTTGAANLLANYASNPDSRKLPFYKNMRFPPYTVV
                     NNRLLVDAYFVRDYHGDDSTIFASGSNKNGMSPGNWSCPVSQSIPDKNEILDVMIHVR
                     KAGPNITDSMWLFGGISIENTTGNRYFDFELYQTDIFYDRATRKFNNYGPDAGHTRWQ
                     FDAAGNMLAPGDIILTAEYSSSALTMVEARIWIDRADLLINPVGFNWSGSFDGASSGS
                     QYGYAGIQPETAGAFYTGLQSSNNTWAGPFSLILGDNSQLTNYAARQFMEFSVNMTKL
                     GLDNAKIFGGDDCLMPFRRVIVKTRASTSFTAELKDFVAPFDFFIAPAAQIETATPMI
                     CDTGSLSNIYVTNPIPTSIYQWTTPNGRFVGPATGPSVFVDTPGVYIVTQYLQISCGA
                     YATDTITVGRLPDCNVLANNLINFKGSYTNPAVRLEWKVLNNALARAFYIERSTDGTT
                     FTTIGMESPRPGWEGEAIYNTTDDLSGLNTPFVYYRIRMIESGNVQLLSSVIKFGNSR
                     TDNNPIRVLSNPVKDQLQLMIRAEHAAPGTLSLFDQSGKRVFTTTISLQKGISSFQFN
                     CMTGLPEGLYQIVLATGNRISSARILHKN"
     gene            33062..33814
                     /gene="rsmA"
                     /locus_tag="PLU11_00270"
     CDS             33062..33814
                     /gene="rsmA"
                     /locus_tag="PLU11_00270"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPN57564.1"
                     /translation="MYTLKKSLGQHFLRDENICRKIIAALQEHPYTQLLEVGPGGGAL
                     TKYLVQLPDIDFKAVELDDEKVNWLLQTYPVLEGKIIHQSFLDIDTPYQGRFTVVGNF
                     PYNISTQILFKLLDWRDQVECMVGMFQKEVAQRVAAKEGNKVYGVLSVLIQAFFDVEY
                     LFDVNEQCFNPPPKVKSGVIRLLPRKTPLELTDEKKFFLLVKTAFNQRRKTLRNAVKG
                     LFDAAILEDELFNKRAEQLSIEQFASLICKMR"
     gene            33811..34743
                     /locus_tag="PLU11_00275"
     CDS             33811..34743
                     /locus_tag="PLU11_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN57565.1"
                     /translation="MKKIIITGRAHPVLKDSLEKQGYEVLDMPDISAGELGGLIADAD
                     GLVVTTRIPVNKQLLDKAVRLKWIGRLGSGMELIDQSYAESRGIQCISSPEGNRNAVA
                     EHNLGLLLNLLNRLSIAQEEVKKGLWKRVENTGTELSGKTVGIIGFGNTGSSFARLLE
                     PFGVTVLAFDKYKYGFGKGYIKEASLEQIARYADVISLHVPQTPETTGMLNDDFFQTL
                     QRKPVLLNTSRGKVILIDALIKALKEERISGAGLDVLPNEKLSTYTEKEQEELNWLLA
                     RPDVIITPHIAGYSEEANYKMAAVLLEKLGIRIS"
     gene            34790..35245
                     /locus_tag="PLU11_00280"
     CDS             34790..35245
                     /locus_tag="PLU11_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57566.1"
                     /translation="MRQWITAIAGLLFIGGLFSSCSKNLEDRMTGTWKLEEASRRLST
                     GTSPFRTGYEDGLFKLDDNGLATYIEGSDTLTGYWRAGKHNKGIYNNSEGSWQARDMR
                     YLLISVSNTNIHRTLEWKFDDIDFHSHNKGLRAEQYTLGFDRIYEFKKR"
     gene            35367..35840
                     /gene="greA"
                     /locus_tag="PLU11_00285"
     CDS             35367..35840
                     /gene="greA"
                     /locus_tag="PLU11_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPN57567.1"
                     /translation="MSALQYVTKDTLEQMKAELQRMKAVDRPAASRAIAEAREKGDLK
                     ENAEYDAAKEAQGILEAKIAAMEGAMANVRVLDESSIDTSKVSILTKVTLTNLNTKKQ
                     VTYKIVSEKEADLKAGKISVTSPIGKGILGKVIGDTAEVQAPAGILKFRIDEITA"
BASE COUNT         9769 a         8179 c         8535 g         9579 t
ORIGIN      
        1 gatcttgggc gccaccagct gcgtctcgaa gcgcgcgatc tgatgcaatg gggatgggca
       61 tttcctatat caccggcaag cgttgtctgt attatggtac cgggcgaagc agtgatgtgt
      121 tttcggttga gctggatagt aagggccggt tttccggtaa gccggaaaag gtattttcta
      181 tcgcggggat gggtccatcg ggagatgata aagtgcggcg gatccggaca gacaatatgg
      241 gtaatttgtt tgtttacggg atgtctttca actataatct cataccacaa cgggagaaaa
      301 aagagactgt ctattctttt ctgtatgacg ccggatccga aagttggatc agtaaatagc
      361 gcactaacgt tgattagttt gagttttata attttctgat ataactaagt ttactaattt
      421 tacacctata tttatttagg tgtaaaatta gtaaacttag ttatatcaat ttcatatgaa
      481 taaaaccaac tatctgctgc ttttagtagc ctctgtattc ctgcatcaag ttgtatcggc
      541 ccaggtttcg ggcattgttt tcagagatta taatggaaat ggcacaagac aggccgcgac
      601 agcttataca gagccactgg tgcaaggtgt ggtagttcgg gcatacaatg cagctgaagt
      661 gttgctcgca tctgccacaa ccagtagtgt tggcgcctat tcatttacag ccggacaaat
      721 tccgtcaggt acagcgatcc gtcttgagtt tacgatcccg tcttccggat cctgtctcgt
      781 gaattccgca tttgattttg catccggatc cggtgccacg catggtactg cagttcgttt
      841 tctgacagcc ggtgccggcg cggttaatat caactttgca atcaacgatc cgaatgacta
      901 tctctcaacc gctgctccct tcaccgctgc ttttttattt accaaccgtt atcattcagg
      961 taatgcaacc ggttcgggaa catcagcagc cggtggtgtt ttttataaaa tgagttacac
     1021 cgttaaggga caaaccactt tacctgcttc tggtttactc gccaccaatg cgcagatcgg
     1081 cacctgttat ggtattgcat acagtaaaca agcggaccgt gtatttactt ctgctttttt
     1141 aaaacgtcat aacggtctgg ggccggcaaa tggcacattt aataatgcac cgggtgccat
     1201 ttatatgatt aatcccaatc tgacagcggg aacaggtgca gcatcctact tttccagtct
     1261 ggatgccctg ggttatccca cacataactc aaccgggtct ccagcttatg gcgccggtac
     1321 ttcatataca cttaccggaa ccggatatgg ggctactgtt tcttatccgg ccaatggtct
     1381 tggtgtaatc ggaaccaatg tgaacagggg cttacccaat gatagtacaa cccgcagcaa
     1441 tgacccggct gcttttggtc aggtaggtaa agtgagtctg ggtgatatgg agatcagcga
     1501 tgacgggcag tttttgttcg taaccaattt ctatgatcgt aagttatatc agcttcagct
     1561 gaacaatgta aagaatccga catctgcaac ggtagtgaac agttggagcc tgcccaatcc
     1621 gccattacgt tccacatccg gcattccgtc tgcagcagct acatacaccg gtgccagtga
     1681 tggaactaat ttttatgatg gcacccgcgg attgcaacgt ccgtttgccc tgaaatactc
     1741 aagaggtaaa ttatatatcg gatctgttac aactggtgag ggaaccggag ccgtaagtac
     1801 acaagataat aataccggta atccggaata taccgatctc tgggcctatg tgtgggaatt
     1861 caatcccacc tccggtacat ttacctctac tcctgttttg caatttccgc tcaacttcaa
     1921 ccggggtact aacggagacg gtttatctga aacctggaga ccctggacga acacgctgcc
     1981 ttctccctgg acaggtacag caccaggttt ttcgcaatac cagcaaccca tgttcagcga
     2041 tattgagttc gacagtgatg gaacgatgat actcggtttc cgcgaccgtt ttggtgatca
     2101 gtcgggttat gatcagagcg gcctcaatgg tactgtgcga ttcgccggtc aggccatggg
     2161 tgatatgtac cgggcttatt ataacagcag cagttgcgtt tttgaattag aacaaaatgc
     2221 gaaagaggga ccttccagtc ccaaagtggc tacggcgggt gccaataatg gcgatggtcc
     2281 gagcaattat accaccaata acggggagtt ttattacagg gatgaagtgt ataatgcgaa
     2341 taccggcgct actgtgggca catttcacct gaatacggtg agtggagcgc tggcgataat
     2401 caaaggaaca gacagtgtgg tggtaaccac catggacccg ctccgtgcct ggtcatccgg
     2461 tatcagttgg tttggcagtg ccggtggtga caatggccgg gactatgaaa tgtttgccgg
     2521 tgccggagca ggcacattcc ccgcacctgc agtcggcgca acaggtaagg ccaatgggct
     2581 tggtgatatt gaactgctga gtgcggtagc accaacggaa ttgggtaacc gcttatggaa
     2641 tgatgcgaat ggcgatggca tacagaatgc cggggaaagc ggattggcaa atgtcaccct
     2701 tgaattatat agtaacgggg tggatgggct gccgggtacc ggcgatgatc aattgctggg
     2761 caccaccact accaatggtt cgggtgaatg gtactttaat acaggcaatg ttacagatgg
     2821 agatccaact acaggcggta atcaggcagg gccacaaccg ggtataccct ataatatacg
     2881 ggtgggcagt gccgactgga ccggcggcgc cggtaccggc gatctggcta actaccggtt
     2941 atctgtaacc gataaattat gtgcaggggc agcggacctt agcgataacg acgcatcact
     3001 cagcagtggt aatattccca tgatcagtct gacgatcgct gctgccggac aaaataacca
     3061 tgatcttgat tttggattca cccccctggc aagggtaggg gataaagtct ggcgcgatga
     3121 tgatcaggac ggcgtgcagg ataccggcga acccggcgtg gccggcataa ccgtgaccct
     3181 ttatgcaagc gatggtacaa ccgtattggc caccaccacc acagacgcat atggcagtta
     3241 ttatttcgac cagctcgcgg caggtaatta tatcattggt gtaaccttac cggtgaatta
     3301 ccagtttacc agcagcacag gtaccagcga agcagatggc accaacagtg atgtgagcag
     3361 cagcaccgga aagactactg cgattacctt atctgctgct gaaaatcaac aacagattga
     3421 tgcaggtatt atttatacac cttcgctgac caatagtatc ggagatcgta tctggctcga
     3481 cctcgatgga aatggttctc agaacgggac tgagcccggt cttagtggtg tgaccattac
     3541 gctttatgct tcagacgggg taactgttat tgcaacaact gtttccgatg caaatggtca
     3601 gtttaacttt acaggccttc ccgcttctac tgattatgta ctgggtatca cacagcctgc
     3661 aggcctgttg ctgaccagca gcagcggaac aaccggggcg aataataccg ttaacagtga
     3721 ttttagtagc agtaccggaa aaaccggact ggtgagtagt ggaaccgccg gcagccaggt
     3781 aaaaggaatc gatgctggtt ttgttactca acccgcagcc agtgccgtta tcggcgaccg
     3841 tgtttggctg gatatcccgg gtggtacggc cggagtacag gatgccactg aacccggaat
     3901 tgccggagtt acggttaatc tttaccgtga tagtaatggc gacggatcga tcagtggagc
     3961 tgaagctgcc acgccattca gtacaactgt ttcggacgca tacgggcatt atatttttaa
     4021 caatcttact gccgccgtaa gtacgggcag tctgtaccag gtggagtttg tgttgcctgc
     4081 cggctatacc cgtactactg ccgatcaggg tgccgatgat gcagttgata gtgatgccga
     4141 tgccacaaca gggcgtacca ttgcttatcg attacttccc ggtcagcgca atctctcact
     4201 cgatgcggga cttgtgctgt cttctcctgc cggaacttca aggattggtg ataaagtctg
     4261 gtttgatgcc gatgcggatg gtgtacagga tgccaccgag ccaggcatcg ctggtgttac
     4321 cgttactttg tataatagca gcggtacagc agttgcaagt actacgacag atgcaaacgg
     4381 caattatcag tttgtacaac ttgctgccgg aagttatagt gttggtttca gcaatcttcc
     4441 tgcaggttat agctttattc ccatctggtc taccgatgat gcaaatgcca cgaacagtga
     4501 tgtaaacccc ggaacgggta gaacagcgct gatcagcctc agtgcaggcg aaaatgaaac
     4561 agatgtggat gccggtttat atgcaggtat tgcagcagga cttggttcca taggaaataa
     4621 agtatggtat gatattacag gcggaacgtc cggtgtgcag gatgccggtg aaacaggcgt
     4681 gcccggtgta acagtcagtc tgctggatgc tggagcggat ggtattgtgg gtaacgggga
     4741 cgatggcagt acccgcagca cgatcaccaa tgcaaaagga gaatatattt ttaccgatct
     4801 gcctgcgggt aattatgctg ttcaattcag cacattgccg gcctcttata ctgcaactac
     4861 acaaaatgcc ggtgctgatg atacccgtga cagcgatggt ggtgccattg gtacaggtgg
     4921 agcacctgcc ggtgcttcga gaacggccgt attgagtctc acagaagggg aggataatat
     4981 gtcagtggat ctaggacttg tgcctcccgc aggtaccaac tcattgggca acctggtatg
     5041 gtttgatgcc aataatgacg gattacaagc tggtgaacag gggatgaccg gtatactggt
     5101 tactttatac aacggaagcg gtacagcggt ggcaacaacc gttacgaatg aaaatggtga
     5161 ataccttttc agcggtcttg ctgatggaag ttatagcgtg ggctttacga atctgcctgc
     5221 aggatttgat ttcagtactc cttccgctac taatgatgca acgggaagtg atgccaaccg
     5281 cgctacagga cgcacaacga ctgtgactct caatgctggt aaccgtaatg accgtacact
     5341 ggatgccggt ctcatcagta caagggccgt cattggaaat tatgtttggc ttgatacaga
     5401 tgggaatggt atccaggata atgatggctc agaacctccg gttccgggag ttaccgttac
     5461 cgtgtatcgt cccggattcg gtcccgatgg tattgccggt aacagcgatg atgccttacc
     5521 ggtagcgagt atgatcaccg accagaatgg tgaataccgt tttggtaatc tgctgcctgg
     5581 cgattatcag ttggagttcg gtaccctgcc agccggactt gtattcactg ccaatgatgc
     5641 aggtggcgat gatgcactcg acagcgatgc caacccggcg acaggtctta caggaaccat
     5701 tacactgagt gccggtgaaa cggacctttc tgctgatgcc ggattattta aacccagagc
     5761 tgtaattggt aactatatct ggtccgattc aaatggcaac ggtattcagg atccggatga
     5821 gcgcggcttt gccggtgtgc tgattacctt gtttaataac cttggcgtgg cagtagcggc
     5881 gaccataaca gatgctcagg gctggtacca gttccctaat gtagcccccg gcacttacag
     5941 tatgcagttt accaacctgc ctgctgctgt cacatttacc acagcggacg ctggcggtga
     6001 tgatgcagcg gacagcgatg tatccggagc taccatccct ggaattacag taagcactac
     6061 cactactaat ctcacttttg atggtggcct gctcaatgcc gtaacacttc ctgtatttat
     6121 tcaggcattt acggcagtgc ctgccggtca aacggtacag ctcaaatgga aagtatcaga
     6181 agaacaactg gtggccgcct atatcgtgga gtatagttcg gatgccagca actggactgt
     6241 attaggtagt gtagcggcaa cgggggctga gcagtatagt tacctgcacc tttcacccat
     6301 aaaaggagcc aacttttacc gtatcagagt ggtggatgca gatgcttctt acaaattcac
     6361 cgaagtgagg agtgtatatc tgcaaaaagg caactcgctc ctcgtttatc ccaaccctag
     6421 tgtcaacgga ttggtgtatg tacgtttacc tgaacagatc gttgggaaag gtgttacgat
     6481 aacggtttat gcgctgaatg gtcaacaggt aacccgtgtt tacctggcag cggccggaca
     6541 aacagaacaa ctgaagttga atggtttggc aaacggccag tatatccttc agttaaaggc
     6601 ccctgctgcc ggatacagcg aaaccagccg gctcgaagtg atgaagtaaa atagtactgg
     6661 ataaacgtat aaaagggttg tacagattgt acagcccttt ttcatttact gcagctgtat
     6721 cccgtggatt tatccgtagc ggttcggaaa ttcacgtagt ccgacgaagt atatccacgg
     6781 agaattccgg ggaaaatgat tattttttct acttcccgga ttaaacttta ttttctttat
     6841 atattgataa tcaacagatt aatttattac ttagtaatat tactacagca tttacggtat
     6901 tttttacgaa aaaatgcgtg aaatacttgc aagtaaaaaa actgcgtatt atgtttgtat
     6961 cagaatcgaa caaatcctaa gaaaacaatc gaaatccgac cctgaaacta cttcttctta
     7021 aacgggatat tactcccggt acgtttcaac ttctttttac gggtttcatt gcgatctgag
     7081 attatccggt tcttaccctt taataatctg taacccctga gcaatgaaaa caactttaca
     7141 cattaaccgt gctatccgtc agttcctggt gctgactgtt atttctgcag ctaccgcaac
     7201 tgcttccgcc caaacctcac aagcaatcgg tgaaatgaca tttgagaatc ctgcccttga
     7261 gcctacttca gtcgcaactg gtacagccaa tggagcagaa taccggttct cgaatgtgac
     7321 tcctcagatg gatgctataa tgaccatcac aggacgttct tcatcgcagg tagtgatcaa
     7381 caatctggat ctgaccggaa atggttttaa taaggctttt caaccgcaaa tccgttacaa
     7441 caatggaaat atcaacagtg cgggttcatg gtggatcgag ttccagtttc agtttgtaaa
     7501 caagggtaca aagcttcctg cacaaatgag tgagtttcat gtaaccggtc tggatattga
     7561 tggcaatggc agccgcctgc gtgagtggga tgccttctat ggtggtgcat catatacttt
     7621 ggagaataat tctcagctga ctgtagcaaa tctgttgggt acactgaatc tgcctttact
     7681 gaatggtaaa cagttcctgg gctcacagtc tgaccgtaac aatattgata ctgctgctac
     7741 tgatgtcatg acaaccgtta agtatactaa tacaagtacg atggtgatcc gcctcggtgt
     7801 aaccactacc ggttctgcca gcaatgccaa ccgtatgtat tctgtatggt tcaaaaattt
     7861 tgcttacatc gctccgatta gcacactgcc agttaagctg acggctttca acgcctcttt
     7921 agctaaggat aaagtaaacc tgaaatggga aactgcttct gagatcaacg tgagtcattt
     7981 tgaactcgag cgcagtacgg atggtgttaa tttctcccag gctgcgatgg tgttttcata
     8041 tggtgcagcc gatacaaaag ccaactatgc ttatagtgat aatgtggcct ccttccaggt
     8101 accagtggtt tactaccgtc tccgttcagt ggatattgat ggcaaaagtg aaatctcaga
     8161 aacacgcatc atccgtctgg caaaagatca ggccaacagt atcagcctgc tgacctatcc
     8221 caatccggtc atgaatgaac tgcgggtaac tgtacccaac aactggcaga ataagcagac
     8281 tgttttcgaa ctgttcaatg cgaatggaca gatcgccagc cgcaaagcca ctaccggctg
     8341 caaccagaca gagacaatca acaccagcaa cctggctccc ggtttctaca ttgtgcgggt
     8401 tagctgtgat ggccaaacag cccagcagaa gatcgtaaaa cattaataaa caatattttt
     8461 cataggggtt ccgaccgtcc tgccaaaagc gggacggttc ttttttgtaa aaaatatttg
     8521 gtttatatta taaactactt tacgtttgtc ttcaaatcaa gaatatgaga cgcggaaacc
     8581 ttaacctgct cttcaccctt gtccttgtta ttaccggcct tccggtacta tcacaagtaa
     8641 ctatcaaagg cgtgataaaa gacaataaaa acaacccggt agccggagcc agtatatcca
     8701 taaaagacag ctacgatgga acaacctccg attcggccgg acgctattca tttaagacaa
     8761 ctgagagagg tcaacaggta ttacaggtga ccagtattgg ttacaaaaca gcagaacaat
     8821 ctataaaact tgaaggcgcc acacttacag tggatgtagt aatccggcag gaaatcagcg
     8881 agctcactgc cgtagtattg tcagcaggta cttttgaagc cagcgatcgc aaaagagccg
     8941 ccgcggtact cgatccgatc gatatcgtga ccaccgcgag tgcaaatggc gatattaccc
     9001 aggccttaaa aacactgccc ggtgcccaac aggtaggaga gagtgaagga ttgtttgtaa
     9061 gaggaggtac cgccgccgag accaagacct ttatcgatgg caccctcgtg aataatttct
     9121 tcttcagcag tgtgcccaat attgcccagt ttggtcgctt ctctcccttt atttttaaag
     9181 gtacggtctt cagcaccgga ggttattccg ctttatatgg tcaggcctta tcctccgcac
     9241 tgatacttga gtcaatcgat ctgcctgaac gtacatcagc caatctcgga ttgagtgtga
     9301 tcggcggcag tgccggatac cagcatctgt caaaaaataa taaatcctct ttcggtgcga
     9361 gctattccta taccaacctg gcgcccgcat ttgcggtgat caaacagcga caggattatt
     9421 ccactgttcc caattaccat aatgccgatg ccaatttccg gatcaagacc tcaaagaccg
     9481 gtttcctgaa atactatggt tacttcagca gcaataaact tgcttttaca gtgaacagta
     9541 ttgattcact gggctaccgt gatgagtttg ccatcagcaa taccaatcat tttcataatc
     9601 tttcctggcg ggagaatctt ccgaaaaaat ggcggatcaa tgcaggtctt tcgtacacca
     9661 ataaccagga tgatatccgc agtgctatgc aaaacaatgc cggcaatgaa gtacaattat
     9721 ccggactcga agccaaaacc tttcaactgg ataataaagg gaattacttc aacgccaaac
     9781 tggtgctcga aaagaggttt aaaggactca gcggtatccg ctttggttcg gaatataatt
     9841 tcagtaaaga caaatcagta tatacggcat tcaacaatca gaactatccc ggtggactca
     9901 gggaatccat cagctctttg tttgctgaat ccgatgtata tgtcaccaac gacctggccc
     9961 tgaaaggtgg gctccggatg gaacattcac agtacctcgg taaatttaat ctggcgcccc
    10021 gcgcatcgct ggcgtataaa cttggtaaag gcagtcaggc atccctggcc tatggggtct
    10081 tttatcagaa tcccgaacgc cgttacctgc cttcgcccaa tgatttatcc ttcatgaagg
    10141 cagcgcacta tatcctgcaa tatcagaaag tactgaatca gcaatctttc cgtgctgaag
    10201 tgttttataa aaaataccat aacctcatca agaccgattt tgtgggcttc accgaagcgg
    10261 cggtcagtac caatggattt ggtgatgcaa aagggatcga gtttttctgg cgcgataaaa
    10321 agactattaa gaacttcgat tactggatca cgtattcctt cctcgatacc aaacgcgatt
    10381 ttctgaactt cccgttcgcg atcacgccca atttcgctgc caaacacaat ggctctattg
    10441 ttaccaaaaa atttgtacag aagatcaaga cccagttcaa tatgtcatac acgtacagta
    10501 gcggacgacc ctattataat atcagctacg aaggcaatgc ctacaaattc accgaccggg
    10561 gcactattcc ggattttcac aatgtgagtt ttgccctgaa ttacctgccg acgatcggga
    10621 agaaagacgc taaatccttt gcagtgtatg tactgcaggt gagcaatatc ttcaatatca
    10681 aacaaactta cggttaccag tactcgtata acggaatgcg gaaagaaccg atcctgccta
    10741 cttcgaggat gttcgtgtac atcggcgcct ttatcagttt tggaatcgac cgcaccaatg
    10801 aagtaattga caacggactt taatagttaa ctataaaacc taaaaagtaa tcaatatgaa
    10861 aaagttactc ttcctggcat tgacagccct ttttttacaa ccggtatttg ctcaaagtga
    10921 aaaattcacc ggtgctatga aaaagaatat cgcagaaatc gacagtatct tcaaatccaa
    10981 tgaaatgaac cggctgaacg atatcgccgc tacctttgag cggatcggcg atgcagaaaa
    11041 gacccagtgg ctgccttact attatgcggc ttattgccag gtgacactcg cctatgttaa
    11101 aaacgaagtg gccaataacg atatcatagc cgataaagtg gaccagctga tcgctaaagc
    11161 agaagcattg caacccaaaa acagtgaact gagcctgctg aaggccatga acacatccct
    11221 gcggatgctg gtgaatccca tgcagcgctg gcagcagtat ggccccaaag tgcaacagta
    11281 cacccaggat gcaatgaccc aggatccaac caatccgcgt ccgcattatt tcagcggggt
    11341 ttcacttaaa aatacacccg aacaatttgg cggcggttgt ggtacagcca aaccaatctt
    11401 tgacaaagcc cttgagcttt atgcagcttt caaaccggcc agtgatatcc atcctaactg
    11461 gggccttcag gtttgtcaga gagaagcaga aggatgtaaa taattaacca gcacaaaact
    11521 gcagttatga aagaagccga aaagcccatg accgaacagg agagcctggc gctgatcacg
    11581 tctatgatca accgtgccaa ggatgtgaat cacagcacag gtctcacctc tataatgtgg
    11641 ggattggtga ttatgatatg ctccatcgta aaactggccg agattcattt tgaattcaga
    11701 ttgccatttg atatttacat acttactgtc atcgcgatag cgcctaccat ttacttttct
    11761 attaaacaaa agaaagagag cagggtgaca ggctataagg atagttttat tgacaatacc
    11821 tggctggcat tcggcatcag cattttcctg ctttcattta tcatgaatgt actgtttgca
    11881 gaactgcggc cgatgaaaga cagtgtggaa ctggcaaccg gtaagaacct gttatacatt
    11941 ttgtatgaat acatgggttc ttttttcctg atgctttacg ggctgcccac ttttgtcacc
    12001 ggggttagca tgaaattcaa acctatgatt ctgggtggaa ttatttgctg gatcagttgc
    12061 ctggcggctg tatacacgcc tgtaaaaatc gacctgatcc tgacagccat ttccgccctt
    12121 tttgcctggt ttattcccgg ggtcatactg gaaaaggatt atcgtaaagc caaaaaagag
    12181 ttgggggcac agcatgttta aagacctcaa tcccatatta cattcgcagc tgcgcctggc
    12241 ggtaatgtca ctcctcatca gtgtgaagga agcggagttc acttttatca aagagaaaac
    12301 caatgccacg gccggcaacc tcagtgtaca gatccagaaa ctgaaagaag ccggctatat
    12361 cgaagtcgtc aaacaattcc gcgacaacta cccgcagacc ttatgtcata tcaccccggc
    12421 cggtattgcg gcttttgaag agtatgtagc gagtttgcag gggtatctgg ggggaaggaa
    12481 tgagtaatga gtaatcagta atgagtagct cgggtaaata cgcgacagtt tagagacagt
    12541 ttcaaatctg taaagatttt tccactctta ctcattactg attattcatt actcattaaa
    12601 aatgagaagg ccctttagaa aaagggcctc atctcttttg gatactctaa gtgtatgaaa
    12661 gatttatcac cggggaaacc ggtgttgatt ccggacaggt tgcaacacat aggccggagc
    12721 tttttttgcg gggatattca gcatacgctc taccgctttt ttcaaacggt cggtttgctc
    12781 ttttaaggcg ttattgattt tttgtttcat ggtatgcagt taatggttga atgcagcatt
    12841 ctttagcccg ctgttaccgg ggctgaataa tatacctgtt tgtccttact gataattttt
    12901 acccggtaca ccgtcttctt cccggtattt ttttcaaaaa agaaatactc ttcagcatct
    12961 gtcttatccg tactgaatac cagggcggcc atactgaagt ttttgccatc cgtgcttttc
    13021 tccacttcaa aaaggttgac ctgctgattc tcttctgttt tccagttcag ctgaaccttc
    13081 tgattgttta caacagcttt cagttccaca atggccggaa ccgttgcaac ggtggtggtg
    13141 cttgtaaaag gggatacatg cagggattga tcctgtgccg cggtggacag ctgcaggaaa
    13201 agggcaaaaa cggataaaaa tttaatagac atttctcagg cttttaattt catttgttag
    13261 cccacagaaa tattggatgc ttcggcagat ttaccagcag acttgcttta aacggccttc
    13321 ccggaaggtg gagcggatgg tggcccgggt acggacgaat atcgtagttt tacgtggtta
    13381 aatgtggttt tccgaatcgc aaacaaacct aatccttgta cgtaataatt gcaagtattt
    13441 ttagataatt tttttagaca aataggtaaa tacttcatta acagggacga acctttaata
    13501 catttgtccg ttaactatcc cgaatgagcg atgtaatccg ggtcaaagac ctaagtaagc
    13561 attttggtga gctgaaagca gtacagcaac tttcatttag cgtgggagag gggcaggtct
    13621 atggcttcct cggacagaac ggtgccggaa aatccacgac catccggatg ctgatgaccc
    13681 tgatcgaacc taccggcggt gagattgaaa tcttcggaat gaacctgcgc aaacaccgga
    13741 aggaaattct ccgccagacc ggtgccatca ttgaaaagcc cgatctctat aaatacctta
    13801 cggctaaaga gaatgtccgc atcttttcac gcttgagtgg tgttaaggcc tctgagaagc
    13861 agattatgga tcagctttca ctggtgggac tggccggtcg tgccgacagt aaagtaaaga
    13921 cctactcaca aggtatgaaa caacggttgg gactggcttg tgccctggta cataaccccg
    13981 ggctgatcat tctcgacgaa cctaccaatg gtctggaccc gcaggggatt gccgacatgc
    14041 gtcagctcat ccttcacctg agccgcgacc tgcacaaaac cttgctggta tcctctcacc
    14101 tcctcagcga aatggaaatg gtggccgatc acatgctgat cattcataaa ggagaaaaag
    14161 tggtggaagg aaaagtgagt gaactcctcg atcctgaaaa gacaatggtg gaactcctca
    14221 caccggatat aaattcagcg attgctgaat tcaagtcagg tccctggcaa cgccacctga
    14281 aagaaccggt aaaaggtacg gtcagacttg aatgtaatcg caacgaaata cctgcattga
    14341 ataaatacct cgtggaaaaa ggtataccgg tgcaaagtct gcgtccttta aattcgctgg
    14401 aagactattt cttatcctta accaaagtgt agatgtttat cgaactgctt aaaatagaat
    14461 tgtacaagat cttcaagcgc ccgcgcactt tcattgcgtt cggggccatt gcagtgatca
    14521 tcttactggt acagtttgcc ttgaaagtaa acggaaagga ttttgtggaa ttttataccg
    14581 acagccaggc cgatacattt gatattccct ttgataaaat actgaatggt tactttgtat
    14641 gtgtggccgt tttgcacatg atcctgatcc atgtgcccct gctggtggcc ctgattgccg
    14701 gtgacatgat ttccggagaa gcaaatctcg gcacactccg cctgctggcc ggcaaacctg
    14761 ttacccgtac tcaactgatc atggctaaat tctgggccag tactatttat gtgattttgt
    14821 tattgatatg catggccttt ttcgcactcc ttggaagtat gatcatcttt ggtaccgaag
    14881 acctggtggt atttaaagaa accgatatgc atatcatgaa atcaggagat gtgatctggc
    14941 gtttctttgc ggcttttgtt tttgccgcac tcgcattgac caccatcgct gccatggccc
    15001 tgatgttctc cgtgttttcg gaaaactcac tcggtccgat tgtggccacc gtttgtgtag
    15061 ttatcgtatt caccattgta cagcaattgc aggtgccggt atttgaacaa accatcaatc
    15121 cctggtcctt caccacccac atgctgggct ggaagggatt cttttatgta gaaaaaaatg
    15181 cggaaggggc tactattccg ggctcgattg aaaacccggc tgccctcgtt cgcagcggac
    15241 tcatcctcct cggttatatt gttctgttcc ttacgatcag tatccgcggc ttcaataaaa
    15301 aagatattct cagttaatac cgttttatga aacaacgtct cctgttcttt tttgcttttt
    15361 cactgttctg cacacaactg ccggcacaag ctgccaagga actgttgcag aaagtaaaag
    15421 ccaagtatga taaagtgaat gattatacgg ccaccggcaa gatgaagacc aatgtgatct
    15481 ttatcaaagc gccgattgcc aatatcaagg tctactataa gaagcctgat aaactaaaga
    15541 tcaagaatga aaatggcgtg tcgtttattc ctaaaggatc ggcaaatgtg aatatgaaca
    15601 atgtactggg tctttccaat tttgaggccg tggaatcagg tacggaaaaa gtggcgggca
    15661 tcaactgcaa agtgatcaag atctttccac tgagtgatga ggagaatatt acccgcgcca
    15721 cactctatat tgatgaagcc cgtttactga tgatcaaatc ggtaatcagc acacgtgaaa
    15781 acggcacgta tgaattactg atgacctata aaaaatacgc ggaatggggc ttgccggata
    15841 aagtggaact gagctttaat acccgtgatt ataaattacc caaaggggtg accatagatt
    15901 acgacaatgg caccggcaag ggccagccaa gagatacaaa aaatcagaag gggaaggtcg
    15961 aaatcgatta ttcctcctat gagatcaaca aaggggtgcc ggattcagcc tttaactaaa
    16021 cctactcttc aatgatctcg tccttggcat tggccagaat ggacggggtc atcttgcgta
    16081 acggattttt ccggctttcg tgataaccgt tcctccggtt aatgatatcg atacattcaa
    16141 atatggccgt catgaacgag gatggatcag ctttatcttt tccggcaata tcaaatgcca
    16201 ctccatggtc gggggatgta cgaacagcag gcaatccggc cgtaaagttc actccttcgc
    16261 cggtagccag cgttttaaag ggaatcagtc cctggtcgtg atacatagcc agtactgcat
    16321 caaagcgctc aaaactgcgg cgggcaaaaa aggcatcggc actatagggc cctactacca
    16381 gcatattatt attcttggct tctttgatcg caggtttgat aatcgactct tcttctttac
    16441 cgatcaatcc ttcatctccg gcatgcggat tgagtccgag taccgcaatt ttcggctttt
    16501 caatcccgaa gtccttttgc aaactctggt tgatcagagc cagtttactc aggatttttt
    16561 cttttgtgat atactgtgct acttcaccaa caggcacatg ttcggttacc agggccaccc
    16621 ggaaatcacc ggcacagagc atcatcacca catcttttac cccgaacatt tctttgagat
    16681 agggagtatg tccggtaaaa ttaaactcgg cagattgaat attcttttta tggatcggcg
    16741 cggtaacgag accatcgata tgtttttgtt tcagggcagc tactgcggtc tgtaaagaca
    16801 ggatcgcata cttaccaccg gtttcggtga gctgaccggg cgttacctgg atctcttcct
    16861 cccagcagtt gaacaggttc acctgtttgt ggttaatccg gttgaattcc ttagtactgc
    16921 tgtaattcag gttcagctcc ggcatcgttt tccggtaaaa attgatcagt ttattggagg
    16981 caaagatcac cggtgtgcaa tgttccagga tccggttatc ggaaaaggtc ttgatgatca
    17041 gttcggtccc gatgccgttc acatcgccgc aggaaatacc gatgacaggt ttattcgtat
    17101 tcatggttaa cagtacttag gggttaaaag tacgattttt aaaaggattg aagtcaatca
    17161 cttattttaa aatgagtgct ccgggatttg aaggcagctg taaatcgttc gcttcttttt
    17221 tgccattcag ggttacggat gggagtgtag gattaagggt aattgtgtag tacctgtttg
    17281 ccttttgctt aagtaaatac agtattaata ccgattgtta ttgccgcttt attgagcgaa
    17341 ttttatagtt gcaaaagtta tacccgttac ctatgaaagc cataccattt tacattaaat
    17401 ccctttcact ctgcctcctt ttttttctac tccatgaaat agctggtgct cagctcactg
    17461 ttattcccta tggcagtagc tggaaatact gggcaaatag ccaggcaaat tatccggcca
    17521 actggcatac gaccggtttc gatgatagtg catggccatc tggtaccggt gaattgggtt
    17581 atggagatgg agatgaatct acctgtattc cggcggccag ccctgtagct ggtacacttt
    17641 gtactccaac tggtaataaa tggcctgctt actatttccg aaaagtgatt aatattccgg
    17701 atccggcaat ttataccaat ttcagtttta atattgaccg ggatgatggt tttgtggtgt
    17761 atgtgaacgg ggtggaagta tcacgcaata atattgcagc cggtgctgtc agctatggta
    17821 catccgcaac gacagcagtt gaagatggga tcatcagttt tacagttgct acgtccgctt
    17881 ttgtatcagg taacaataca attgcggtgg aaatgcatca ggcatccgtg tccggttcac
    17941 cacctgtttc aaccagcagc gatatttcat tcaacctgca attgctggcc aatgatgctt
    18001 tttcagccgg actgagccgc ggcccttacc tgcaatcagg tagtcaggat ggaataacca
    18061 tccgctggag aacagcaact gcacagaatt ccaaagtaga agtaggaaca agctatggca
    18121 gctatccgat agtggtgaat gatgccagtt cagtaacaga gcatgttgtt cgggtaactg
    18181 gtttgtcaac agataccaaa tattattatc gtataggtaa ctcaaccgat tcacagttgc
    18241 cggatgccaa tcagtttttt acaaccttgc cggcattcaa tacgacccgt aagatccggg
    18301 tgatcgcctt tggggattgt ggacggggta atgttgccta ccaggacgaa agcctggcca
    18361 gctatctgga ctatctcaca accaatagca ttgacgcagc cgatgcatgg ttattattgg
    18421 gcgataatgc gtataactca ggaacggatg cagaattcac ggccaacttc tttaatatct
    18481 atggcagtaa catgttgaaa aaccataagc tttatcctgc tccgggcaat catgactatg
    18541 gtaacagtgc ggcgaataag gctttgcgga acatggctta tcacaacagt tttactgtac
    18601 cccaatccgg tgaatctggt ggtgtagcgt ctaacaaaca gaatttttat tcttttgaca
    18661 tcggtaatat tcattttctt tcacttgact cctatggtac cgaaagcgat ggcaccagta
    18721 ttgaaacagc tggtggcagc gccctgaaaa catggctgac caatgatctt gccgcgaaca
    18781 caaaaaaatg gacaatcgcc tactggcatc acccgcccta tacaaagggc agtcacaact
    18841 ccgattcgga gtcagacctt gtgaatatcc ggcagaactt tatcagtttt ctcgaaaccc
    18901 gcggtgtgga tatgatcgtt tgtggacaca gtcataacta tgaacggggc tatcttatta
    18961 aaaactatac cggatcctgg gcttctttca acgcaggtac gcatgcagtg agtaattcca
    19021 gtgcaaccta taccagtagt gcaacctgtc cgtatgttta taattctgca cccgccaatc
    19081 atggtaccgt atacgtggta tccggatcag caggggccag tggtactatg aataccggct
    19141 ttggtaccgg gccaatgcct tatgccctga atgatggtgg tgttttttat tttgaagtgg
    19201 aggataatcg gcttgatgca aagatgctcc gtcgtaacgg aaccattttt gatcagttca
    19261 ctattatcaa ggatgtaaac aaaacaacga attataccat ttccaatggc agttcaaaaa
    19321 cattgactgc atcctggccg ggaaactaca gctggagtac cgccgctacc acccgttcca
    19381 taagtgtaac cccgcccatt aatgcagtaa ccaattatac ggtggaagat gagtatggtt
    19441 gcatcactga tcagttcaat gtaacaacct ccagtactct gccagtttta ctgaaaacct
    19501 tcgatgtcag acggatggat gcagttgttc aactgaactg gaccattgtt gcggatcaga
    19561 cggcagcgca attcttaata gagcgttcgc tcaatggccg tgactttgtg atactgggta
    19621 ccgttgatgt gtttccaggt tctggtgagc agcaatatag ttacaccgac caccaaccgg
    19681 ttccgggcac cgttttttac cggctttctg caacaggtgc cggtaccgca cagacttatt
    19741 tcggcatcag gaaactcaat ggtaacacac atacaggctt tgaccttgaa atacggacag
    19801 gggtgacgag tagtattgtt ttggattgca gtacagctac cgatgatcag tatagcctga
    19861 cgatctacac tgtcaatggg gtaaaagtca gtcagacaga tctgtcattg caaaaaggaa
    19921 caacccggaa agtaatagaa cttaaatccg gatggtatat atgtgagtta cgaaacagtc
    19981 gcggagaagc ggttcaacaa aaagcagtag tgcaataaaa cgaaatgaag tttaagctga
    20041 tcatattatt tctgctgact ggtttcatca gtacccggat aaatgcccag gtattgagaa
    20101 cgttcacacc ccggtattct aattcatcgg tgaacggtaa tatcacattt gttgccaata
    20161 atatcatcac ttcttccggg gtttctacga ccgaagcgcc tcctggtggt tctgcagtaa
    20221 ataacgggaa taccggggcc aatatcaata ttgatgcaga tacgctgatc ccatttggtt
    20281 catcctggaa atattattct gcaggagcag cccctgctgg tagctggaaa gaccttggtt
    20341 ataacgacgc agcctggtca tccggaaacg gcgaactggg gtatggcgat ggagatgaaa
    20401 ccacctgtat cccctccggc ggtggaggta cactttgcct gcctacaggt aataaatacc
    20461 tgaccagtta tttccgtaaa acaattaata ttccgtctcc cacggcttat acatcctacc
    20521 agttcagtgt ggaacgtgac gacggctatg tggtttatgt gaacggtgtg gaagtggccc
    20581 gcaataatat gcctgccggc gcagtggcat acaataccct ggcgtcttct gctattgaag
    20641 acgctgttat tacattcagt attgcaaata cctattttgc tacgggcaat aataccattg
    20701 cggtagaaat tcaccaggcg aatgcaagca gttccgatct gtcattcaac ctgcaactgg
    20761 cctgcaccga tggcgtgatc ttcaacagtt cctctgccga tctgagtctg ccatcctgtt
    20821 cgcaagtcct cttcgccggt ctttactggg gtgctacaca gggtacagat ggtatcaatg
    20881 tgtcctggat taccaatgaa actaacgtca aactgaaact gcccggttcg tcaacgtata
    20941 cagatctggt atctacgcaa acggattatc ataacaacac acttgttccc ggattaccgc
    21001 atactgggta ccgttgcttt gttgatatca ccgcattgat gaatacaacc agtcctgatg
    21061 gtacatacac cgttgcgaat gtctgcagcc cggccggtat tgtgaatgca gcaggtggct
    21121 ggacaattgt tattgcctat tctgaccctt caaccattct gcgtaacctt accatattcg
    21181 atggctcagc catcatgaat ggcggcgacc cggccctgca tatcccgata gatggtttcc
    21241 ttacacctcc ttccggaccg gtgagttgtg aactgggggc cgtggtattt gacggggaca
    21301 gggtatcgac cgatgaatat tctttcaaac agaactccaa tccgttggtg ggtgtgtaca
    21361 ccagtcttac acccaatgcc actgcgaacc tgaacgatat gtggaacagt acgatctcca
    21421 aatatggagc cgtggttacc acccggagtc cggctcataa taatacttta ggttatgatg
    21481 ctgatatcct gatcgtgccc aatacactga atgcggtttt aggaaacagt cagacctctg
    21541 ccagtatccg ttttagttcg ccttctgaaa actatatgtt gcaggtggca accaccgcca
    21601 tttcacaata cacaccttct ttcaacctgg tgaaatcgct tactgatgtg aacggcggca
    21661 gcctggtgcc gggtgatgtg atccgttacc gggtagacta tcacaataat ggtaatgacg
    21721 ccagtaatca gtcggttatt tatgataata tccccacagg caccacttat gttccgggca
    21781 gtcttgtaat aaatggggtt agtaaaacgg atgcattgtc cgacgatcag gcagagtatg
    21841 atttcaccaa taaccgggta ctcttccgac tcggtacagg ggccaatgga agtaccggtg
    21901 gtgcactcgc tatcggagca agcggttata ttgaatttga tgtttatacg ccttcttcct
    21961 gcttactggt ttcctgttct tctacaatca gcaacagtgc ccggatcaac tataccggcg
    22021 ctttatccgg tatttcattg tacgattcaa gtggtgtgga tgtgagtggt tgtacagtac
    22081 tgggtccggt aaccgcaaca ctcactggtt cctgtatgcc attgggcgat acgatcctga
    22141 caaatagttg tccggcttct tcagtaaccc tccccgcagg caggtatggc ggctatcgtt
    22201 tttataccgg aatgcccttt ggtgcaggta cactttacga tccggcagtg gcaattacca
    22261 caacacgtgt gatctatgcc tggtatgatg gcccgggttc ctgtgatgat acattgcaac
    22321 tgaatgtcta tattctggct tgtccggata tcgatgatga caacgacgga ttgcccgatt
    22381 acctcgaact taataacccg gtggcactac aggatcatga cagtgatggt acacccaact
    22441 ggaatgatgc gcagtatccg ggctttaccg ataataatgc agatgggttc aatgataatt
    22501 ttgatccaag tgctgatagt gacaacgatg gtatcctcaa tttttatgat ccggattttt
    22561 ccggttatgt ggacagcaat ggcgacggcg tgaatgataa tatggataag gacctggatg
    22621 gcattcctaa ccatcttgat ctggatagtg acaatgacgg aattccggat accgtggaga
    22681 gttttggggt ggatgcgaac ggcgacggac ggattgataa ctatactgat acggacaatg
    22741 atggcttttc ccagaacgtg gatgccaata atacaggtgt cagcggttca tcaacaggcc
    22801 tcggtgcact ggataccgat ggggatgggt tgccgaatta ccttgacctg gatagtgaca
    22861 atgatggtat accggatatt attgaagtat atggagccga tgcatctaac agcgctaaag
    22921 tcagtgtatt tacagatacc gatggcgatg gaataaccga tagctatgat ggcgatgtgg
    22981 ggaatgatac agttgcagaa aatgctgctg ctgctttgtt acgtaccggt actgatgcag
    23041 acaacaatgg acgtgcagac agctggcctt ataagaatat ggacaatgac agcaagccca
    23101 atccgtatga cctggacagt gatggtgatg gaattactga tgtaaaggaa acccagttga
    23161 cggatgcaaa ctgggacgga cgggtggatg gtgcggtaaa tgctgacgga cgaaacactg
    23221 tgctggctgc tctcggatcc atggttttgg cgaatcgcga tgcaacaggc agggccgatc
    23281 tgtatgacat cgattcagac gatgatggta ttccggataa tgtggaagga ttaacaacag
    23341 caggttatct tttgccggct gctacagata gcgatgcaga tggaattgat gaccgctatg
    23401 ataattatgt tggttttggc ggagatggca tccacccttg cgatatagat ggggatggca
    23461 cacccgatta cctcgacagt gatacggatg gcgacggact aattgatatt atagaaggaa
    23521 atgacctgaa cctcaacgga attgtggatg accttgttac cctgaccggt gtggatacag
    23581 atggggatgg gctcgatgac cggtttgata acaacaacag taacgccgaa tcaacctctg
    23641 cttatatggg cgatggtggt accacgtctg gtgatccaac cccgggttca attacaacgg
    23701 ttcagcatac aattattgct tatggctgtg cgtcagagcg cgactggcgt tgtatacctt
    23761 atttgctgaa ttgtgagtat acccgctttg aagccaatgc cgatgggcag tatgtcaggg
    23821 ttgactggac agtgcgatgc agtcagcagc cagcttactt tattgttgag cgaagtgctg
    23881 atcgtcagca ttttgatagt gtgcaaagag tagaagcacg atcagtcatc catgaagaag
    23941 aggcctacca ggtaattgac aggtccctga acactcaact accggtactt tattataggt
    24001 tgaaaacggt attgacttca ggtaaagtaa attattctgc agtaattccg gttttacagg
    24061 ttaacaagcc gaatgatagt atttccctgt ttccgaaccc ggtcagaagc agtatgacgg
    24121 ttggtataag tgtatccagc aatgcccggg ccgtcatgga tattattgat ctgaatggcc
    24181 gtatacattt ccgcgccagc caggttttgg taaaagggaa tactacaatt gaactgaacc
    24241 atgccgtaaa tctcccggaa ggcatgtata tttttcgtgt acaatacaac ggaatgatac
    24301 tgacccgtaa attcaataag ctgcaataaa atttcatgcc ttatttttta taatttgatt
    24361 ttaaagcccg caaaagcggg tttttttatt ctccccgggt actttcccga tacttttgct
    24421 ttataatatc gtaaaactcc tttccgcatt caggattagt acctgatcct tcgtgaaaac
    24481 ctacgtttca gttttctttt taagtaaata ctctgaattt agcacaggaa aatatttagg
    24541 tgttataaga gtattatttc aatcgtataa gtaaacaccg aagttttttc taattataat
    24601 ccaataccag aaaaccagta ccaaaatctg tatcatgaaa aagctttacc accggttgtt
    24661 agcagcggca tttatctgct cttcatttac cagtcaggtt tcagcacaat gtacgaatgc
    24721 caatgtgaat tgggataatc tggagtattt catcaccaat ggaaatacta attacacgac
    24781 atttattaat acgcctactt tgtttgccaa cttggtacag tcgcaacggt ttgctatagg
    24841 tacgaatgca gtcaccatat cttcaaacta tccggcaaac ggaatattgg gggataacac
    24901 ttctcatacc ggagaaaccg ggtcctttgg ttctggtgca gatattaatt ttactgggaa
    24961 tggtacaatt acattaacgt ttgatactgc tgtttcaaat gttcagtttt ctatttttga
    25021 tcttgacctg agtcagaatg ttacagttac ggcttttgaa ggggttactc cgagagtacc
    25081 ggttttgacc aagcctgtcg gcggcactgt gacgattgtt gggaatattg gaacaggccc
    25141 cgccggaggg atagcttcgt cttcatctaa cgcaacgctg aatgtagcag ttgcaggccc
    25201 agtcacatca attacccttg tctttgctgg tacagcggga gatttctgga tttcagatat
    25261 tcaggcctgt gtttacagga attttgccac caattattat gtcatagctc aaccatttac
    25321 aggtcagcca tcctatgtaa tggtggtaca tgatttaaat acggtttttt atgtgaatcc
    25381 ggcaaatgga agggcggttt caatttttac ggaccctgat ccacgggtac gtgagataaa
    25441 taatatcgca tatgatccat ataaaagaat tatttattat tcggtagatg ggttggaaag
    25501 atgtactccg gcggggtcac ctgattccat ccggcatatc aagaagtacg atatgaatac
    25561 tgaaacgatt tcaacattga tcagtgatgt aaagacggcc ccctttaata ttcccacttt
    25621 tcatgccggg cttgaatctg gtggtgcagc tttttatgat gcggatcttt atgtcggggt
    25681 ggaaggctat aagttgagcg ggaccaaaaa ttcgggcagg gagacagtta tttggcgaat
    25741 tgaatttgct tcggattcaa ttactccatc caaggcttgt caggtttggg ggactccggt
    25801 tgacaacgga tcgaataata tccatgacta tgcggatttt ggtctgaaag acggtgttat
    25861 ttatgatttc aattcttcca cggctgttgg cagtggtcga gggcagtatt ggcagataaa
    25921 catgcaatca aatacgacaa cttcaacagt tgtacctaat attttggcta acagacctgc
    25981 gcagactgca cagtcgtgga atggaactat ttattgggtg gctgattcta tcggcgtatt
    26041 caatggtact gttgcgaata cagtcactgg taagagccgg attttaagtt cgccacaatc
    26101 ggtaacctgg gtcggccctg caggagatgc gggggaagcg ttcagaccca aagcagattt
    26161 tggtgatgcg ccttctacat atgatccggt agcactgtct cctgcattgc acgagaagga
    26221 taccgctctt ttcctgggta atgtttttac caacgattgg gactgggaat gggacaagca
    26281 aacatcttcc gatgccagcg gggatggtgt ggatgataat gacggattga cctatgtgtc
    26341 tgtctttgat aaaaccactg gtaattatct tgtacaggca aatatttata ataacaccgg
    26401 agcggatgcc acattaattg cctggtttga ctataatggc aacggggtgt acgatgcaag
    26461 tgaagcactt gccccgatta ccatagccag ctcaccggtt gttcagcaac actatttaca
    26521 ttggtcaggt atcagttcca cgatcgccaa tggaacatat acctacttgc gtctgcgcct
    26581 aacatcctcc tcaaacagta tgaccacgtc aaatccaacc ggctatttta ataatggtga
    26641 agtggaggat tatcgcgtac tggtcgataa tttccccttg tctgtaaacc tgctttcttt
    26701 tgatgcaaaa gctactggac gcaggacggc aaagctgagc tggaaaacga tcaatgaatc
    26761 agagctttcc ggtttcggta ttgagcggag tgcagatggt gttaactgga ccaatattgg
    26821 ttttgcagat gctaagggaa ataacaatac tggcagcaat acatatgaat tcattgatca
    26881 gcaggcgctc aaagggcagt cctactaccg gctcaagctg acagatatta acccggttac
    26941 gtccaggttc agtgagacaa gggcaatccg gattgaggat gttgtacaga atttaaccct
    27001 cgtgccaaat cccgcacaag catcaaccag tatgcatctc agcagtacag cgaatattgc
    27061 agccacgata aaagtgatgg atatgacggg ccgtgccatg cagacgatca aaacagcact
    27121 gaatccgggt gagaatgtga tccagctgaa tggcctggag aagtacccga aaggcactta
    27181 cctgatccag gtggttgctg aggaccttgt tattaataga aaacttgtaa taaccaaata
    27241 aagagcgaat actgaacata cggggactgc tgaaactccc cgtatgacca acgatcctat
    27301 aaccactact taaatccaat ttatgaagaa ccgtttatta agcctggcgc ttatccttgc
    27361 cactatgcag ttcacttccg tttgtcttac ttcctgcggg ctgcgtgatg agcgctactt
    27421 tgagactgag gaaagagaga atgaagaaga agaggaaaat gaagaagaag aacagaacgg
    27481 ggcagataag caactttctg cctggtttca gtcaaaaggc tatcccgatg ccagtaacct
    27541 cactcagaaa tatgccgctg cctgggaaga atttctggag ctcaaaaaaa gatcaccctt
    27601 tcagcagtat gctacccgtt tacagggtat gggcagttgg tcctatgtag gaccgaaggc
    27661 atttggtgga cgtatacttt gtctggccat aaaccctcaa gttaatgccg ctggtcaacg
    27721 taccatattt gcaggcagtg ccggaggggg tatttggaaa acctatgtat cgggtatagg
    27781 agcatctgcc tggcagcctg taataaccaa tactgctgtt ttaggtgtgt ctagtatggt
    27841 atatcatccg acagatacca gtatcatcct ggccggaacc ggtgagtctt accggattga
    27901 aaattattcc agttctcctt attcatccaa tcaggtaagt aatatcggac gtaatgcatg
    27961 gaaggcaagg ggaacttatg gagtcgggat ccttagaagt gccgatgcag gcctgacctg
    28021 gacacaggta ctgataaaat cacaatcaga tctgtttggg attcagaaaa tcaagtatca
    28081 tcccacaatc accggaactg tattcgcctg tgccaccgat gggttatata aatcaacaga
    28141 ttccggtgtt acctggtcgc tgatctggtc cggaacatat tgcagtgata ttgtaatcaa
    28201 tccatcaaat gtcaaccaga tcgttattgc agctggtaat gtaaccaata gtcccaaagg
    28261 cgtgtggagg tcaaccgatg gaggcaccac atttacacag gttacagcag gtggtttccc
    28321 cacagccgct caatttaaag gctcgacgaa tttcaccatc ctgggtacca atacgattat
    28381 tgccagtgta ggcgttggcg atatcgataa taacggaact tataatgaac gcgaagtata
    28441 ccgctcagat gactttggcg taaactgggc ctctgtgtcg aattccacac atgctaaatg
    28501 gcagtcctgg tttgcgcacg gggttactgc tttcccaggc agcacaaata aaatattcat
    28561 gtttggcgta aaaaaacatg tacttgtcat gtccggaaca ccggccacga ccgggacatt
    28621 gacaaatatt gctacgggag ccgccagttc cagttacctg gtagatggca gtcaggaggg
    28681 aagtacaaca tacgtacacg atgatgtaca tgatgtacaa ttcgttccgg gaagcacgac
    28741 tactgcatat tgggccacag acggaggggt ttaccggacc acaaatgcca atgcttcacc
    28801 cattacgggg atgacatttg tttcctgtaa cggtgggtta tacgcacaac aattctatcc
    28861 caccattgct caatcacaaa ccacagccaa cctggcgatc ggtggtttac aggataacgg
    28921 ggttatccgt tataatggaa cgggttgggc ccgggtaatc ggaggggatg gcaccaccgt
    28981 ttctttcaag cccgataatg aacttattgt cctcggatca aatgatacga ggggcgttaa
    29041 ccgttctact aatggaggta caagtttccc gacatcagta atgagttatc ttggcaatat
    29101 tccgaacggg gatgatgacc gtaccgcttt ctgtgcacct gtagtggtcg ctccatccaa
    29161 tacgaatcga tggtatgtgg gttcggacaa tattcatgtg agtaatgatg ccggggtgac
    29221 attcagtggc gacgttgcag gagtgccggg taccagctat atcgaagcct ttcacaaacc
    29281 cgcgattgct atcggtgttt cctattcaaa tgcagatgtc ctttatgttt cagtttcacc
    29341 ttttgcacag aacctgctga cagatggact atattacaac ccgcctgcaa caatcagaaa
    29401 aagtaccaat ggtggtggtg gtttcactac cgttacgacc ggtctgcctg atcgcgtggt
    29461 gaccgatttt gcagtgagcc cgacaaatca tgacagtgtt tttgttaccc tcagcggatt
    29521 cggaaccacc catatctatg tcaccggcaa tggtggatca acctggtcac cccgtggcag
    29581 cggacttccg gatgttcctt tcaataccat cctgatcgat ccggctaatc cgcagatttt
    29641 gtatgccggt tgcgattttg gagtctatgt gagtgcagac cgcggtgcca actggtatga
    29701 tttcaataac aatttatggg atgccacact ggtaatggac ctggctatta cacccggtaa
    29761 taaactcagg gcagctaccc acggtaaagg tgtttttgaa actgcccgct gggatggtat
    29821 ggttacgctc cccgttaact tcatatcatt tgaaggtgcc aatgccggta aaaacaataa
    29881 cctgaaatgg gtagtggatc aggaaagcaa cctgtcacgc tatgaagtgc agcgaagcac
    29941 ggacggattc agttttgccg ccgttaaaca ggttacagcc agaaattccg cgatacagac
    30001 cacctattca atccttgatc cgcttggaca gaatcccgct ccggtatatt attatcgtat
    30061 ccggtcaata gatcaggatg gttcctatac ttattcagag atcgtggcaa tccggatcaa
    30121 tgatggacaa aaatttgaag tactgggaaa tccgttctcg cagtccatta ccatccgtta
    30181 tacaaccagc cgtacaggaa aactttccat tgcgcttaca gatctccagg gacggatagt
    30241 tcgccgggaa gaactgatcg ccgctaccgg aacaggtact tattcaatca ataatctgac
    30301 aggtatacct gcaggtgtat atgtagcccg ttttgatatg gaagggaaaa gcatagcaat
    30361 aaaagtcata aagcaataat atttcaaact cggtagctgg ttattaccgc aggaatttcc
    30421 tgcaagcctc cccttcatag gggaggcttt tttattggaa taactaagta ccgtagtatt
    30481 actactgtga ccgcattttc cgtgacagga agatgaggtg ttttttcata ggtcaacaat
    30541 taatggattg cttaatttta tagaaacaat tttactatga aacacctgaa tgcttgtctc
    30601 ttactcctgg ttattgttat ggcttcttca tttaacaaca tttcatctga tgttaccagg
    30661 agtaaaaatg gctacaatgc aaatacggtt tttgccggtt ttgatttttt cagaacacat
    30721 cgccagggaa aagatggcat cactgctacc tggggttata gcggcggtac tataacaggc
    30781 tatatcgtgg aaagaaccta cgaagatgcg accgatccgt atgcatcctg ggaaacagtg
    30841 gccattgttg ctcatacagg tgcccgttcc tataaagtaa ccaataccaa tgttacaccc
    30901 ggatttattt cctaccgggt tcgggttatg aatggcaata cggaaatagc tgtttcacct
    30961 gtttccacag aacatattgt ggcgcactaa atgttgttgt cacagttgct gaaattttcc
    31021 gattcccata cctcattcca acccttgaaa acgccactgc gcatattatg tttcctgatc
    31081 ttcacctgta atctttcaca ggcccagatc acctatccga ttatcaaagc aaattttggc
    31141 gtggaagccg atctgcgctc caatttcttc aacggactcg tacagtctgg taacgacgat
    31201 tggttcctct ttcccggtac accgggtgcc ggacaatttg tgatcgatac caccggcgcg
    31261 gccaacctgt tggccaacta cgccagtaac cctgatagcc gcaaactgcc attttataag
    31321 aatatgcgtt ttccgccata taccgttgtc aataaccggc tgctggtaga cgcttatttt
    31381 gttagggatt atcacggcga tgattccacc atttttgcct ccggttccaa taagaacggg
    31441 atgagtcctg gcaattggtc ctgtcccgtc tctcaatcca tcccggataa gaacgagata
    31501 ctggatgtga tgatacatgt tcgcaaagca ggacccaata tcacagactc catgtggtta
    31561 tttggtggta tctctatcga gaatacgacc ggtaatcgct acttcgattt cgaattatac
    31621 cagactgata ttttttatga ccgggcaacc cggaagttta ataactatgg tccggatgca
    31681 ggacataccc gctggcaatt cgatgcagcc ggcaatatgt tggcacctgg ggatataatc
    31741 ctgactgctg aatacagcag ctcagcactg actatggtgg aagcccgtat ctggatcgac
    31801 cgtgccgact tgttgattaa tccggtagga tttaattggt caggcagctt cgatggtgcg
    31861 agttccggct cccaatatgg ctatgccggg atacaacccg aaacagccgg ggcattttat
    31921 actggattac aaagcagtaa caatacctgg gccggtcctt tctccctgat cctcggcgat
    31981 aacagtcaac tcaccaacta tgcagcccgt cagttcatgg agttttcggt gaacatgacc
    32041 aagcttggac tggataatgc aaagatcttt ggtggcgatg actgtttgat gcccttccgc
    32101 agggtaattg tcaaaaccag ggcctctaca tcttttacag cggaattaaa agactttgtg
    32161 gcccccttcg atttctttat tgcgccagcc gctcagatcg aaacggccac tccgatgatc
    32221 tgtgataccg gcagccttag taatatctat gtgacgaatc ccatcccgac atccatttat
    32281 cagtggacta cgcccaacgg acgatttgtt ggcccggcaa caggaccatc cgtttttgtg
    32341 gatacccccg gtgtgtatat agttacccaa tacctccaga tttcctgcgg ggcctatgca
    32401 acagatacta ttacagttgg gcggttgccg gattgtaatg tactggccaa taacctgatt
    32461 aattttaaag gatcgtatac aaaccctgcc gtccgtctcg aatggaaagt gctgaataat
    32521 gcattggccc gtgcgttcta cattgaacga agtacggatg gtacaacatt cactacgatc
    32581 ggaatggaat cgccacgtcc cggctgggaa ggcgaagcga tctataatac aaccgatgat
    32641 ctgtccggcc tgaatacacc ctttgtctat taccgcatcc gtatgatcga aagcggcaat
    32701 gtgcaactgt tgagttctgt gatcaaattt ggcaatagcc gcaccgacaa taatcctatc
    32761 cgtgtactct ccaacccggt aaaagatcag ttacaattaa tgatccgcgc agagcatgca
    32821 gcaccgggta cattgagcct gtttgatcag tccggcaaac gggtatttac caccactatc
    32881 agtttgcaga aagggatttc cagcttccag ttcaactgta tgacaggatt acctgaaggc
    32941 ttgtatcaga tagtactggc aaccggtaac cgcatcagtt cagcacgtat tctgcataag
    33001 aactgaccat agcaacaggc tttgttttat tcctttccct attcctgtta cttttgtaca
    33061 tatgtacacc ctcaaaaaat cactcggtca gcacttcctg cgcgatgaaa atatctgccg
    33121 caagatcatt gcagcgctgc aggaacatcc gtatacccaa ttgcttgaag tgggaccggg
    33181 tggaggggca ttgactaaat acctggtaca attgccggat attgatttta aagcggtgga
    33241 gctggatgac gaaaaagtaa actggctcct gcaaacctat ccggtgctcg aaggaaaaat
    33301 cattcaccag agtttcctcg atattgatac accttaccag ggacgattta ctgtggtcgg
    33361 gaatttccct tataatatct ccacacagat cctgttcaag ctgctcgact ggcgcgatca
    33421 ggtagaatgc atggtaggta tgtttcagaa agaagtggcc caacgggtag ctgccaaaga
    33481 aggcaacaaa gtatacggcg tactgagtgt attgatacag gcgttctttg acgtagaata
    33541 tctttttgat gtaaacgaac aatgcttcaa tccgcccccc aaagttaaga gcggggtgat
    33601 ccgcctgctg ccacggaaaa caccattgga actgaccgat gaaaagaaat tcttcctgct
    33661 ggtaaaaacc gcctttaacc agcgacgcaa aaccctccgt aatgcggtaa aaggtctctt
    33721 cgatgcagca attctcgagg atgaactgtt taataaaaga gcagaacaac tcagcattga
    33781 acaatttgcc tcccttattt gtaaaatgag atgaagaaaa taataataac cggccgggct
    33841 catcccgtac tgaaagattc gctggaaaaa caagggtacg aagtacttga tatgcctgat
    33901 ataagcgctg gcgaactggg tggcctgatt gcagatgcag atggattggt ggtcactaca
    33961 agaattccgg tcaataagca actgctggat aaagctgtac gtttaaaatg gatcggccgc
    34021 cttggaagcg gtatggaact gatcgatcag tcctacgccg aatcccgtgg tatccaatgc
    34081 atcagcagtc cggagggcaa ccgcaacgca gtggctgaac ataatctggg tttattgctc
    34141 aacctgctga accggttgtc tattgcccag gaagaagtga aaaaaggtct ttggaaaaga
    34201 gtggagaata ccggcacgga actaagtggt aaaaccgtgg gtatcattgg ttttggtaat
    34261 acgggcagca gttttgcccg cttactcgaa cccttcggtg tcaccgtact ggcctttgat
    34321 aaatacaagt atggttttgg aaaaggatat atcaaagaag ccagtcttga acaaatagcc
    34381 cgttatgccg atgtaatcag tctgcatgtt ccacaaacac cggaaaccac cggcatgctc
    34441 aatgatgatt ttttccagac ccttcaacgt aaacccgtac tactcaatac atcccgcggg
    34501 aaagtgatcc tgattgatgc cctgataaag gctctgaaag aagaaaggat cagcggtgcc
    34561 ggccttgatg tattgcccaa tgaaaagctg tcgacctata ctgaaaaaga gcaggaagaa
    34621 ctgaactggc tgctggcaag acccgatgtg atcatcaccc cgcatattgc cggctacagc
    34681 gaagaagcca attataagat ggcagccgta ctgctcgaaa aacttggaat caggatatcc
    34741 tgaaatttcg taaattcagg gagtatttta aacctttaaa ccggccgtta tgcgccaatg
    34801 gatcacagcc atagcaggac ttttgttcat cggaggatta ttttcctctt gttccaaaaa
    34861 cctggaagac cgcatgaccg gtacctggaa actggaagaa gcatcccgca gattgtcaac
    34921 cgggacctct cctttccgaa ctggctatga agatggtctg tttaagctcg atgataacgg
    34981 actggcaacc tatatcgaag gaagcgatac actcaccggt tactggagag ctggtaaaca
    35041 taacaaaggg atttataata atagtgaggg ctcctggcag gccagggata tgcgttatct
    35101 cctgatcagt gtttccaata ccaatattca ccgcacactc gaatggaaat tcgatgatat
    35161 tgattttcac agccataaca agggcttacg ggccgaacaa tacacactgg gctttgaccg
    35221 gatttatgag ttcaaaaagc gctgattgtt aaaattctag acgcaaattt cccggtttaa
    35281 aaactttttc tacctttata cctgaaatgc aacgcttcag ctaaccctgg ggcgttgtta
    35341 tttttttatt atcaaaagac gagactatga gtgcattaca gtacgtgacc aaggacaccc
    35401 tggaacaaat gaaagccgaa ctgcaacgga tgaaagcggt tgatcgccca gctgccagtc
    35461 gtgccattgc agaagcccgg gaaaaagggg acctgaaaga aaacgccgag tacgatgcgg
    35521 ccaaagaagc ccagggtatc cttgaggcaa agatcgctgc tatggaagga gcaatggcca
    35581 atgtgcgggt actcgatgaa tcgagtattg ataccagcaa ggtatccatc ctgaccaaag
    35641 tgaccctgac caacctgaat accaaaaaac aggtaacgta taaaattgtg agcgagaaag
    35701 aagctgacct caaggccggt aaaatttccg taacctcacc tatcgggaaa ggaatccttg
    35761 gcaaagttat tggcgacaca gccgaagttc aggccccggc aggtatcctg aaattccgga
    35821 tcgacgaaat caccgcctga tctttatcag tcactgaaaa aaggaaaagt cttcgcccgc
    35881 gaaggctttt ttagtaaata aagtagcttt acctataaat gctcaaacct ttatatgact
    35941 ttttaaaccc tcgaacctcc aacctcgaac ctccaacctc caacctccaa ctttcaaccc
    36001 atgactatct tctccagaat catcgccggt gagattcctt cttataaaat tgccgagaat
    36061 ga
//
LOCUS       DAOPMW010000003        34239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1062_length_34239_cov_10.354991, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000003 DAOPMW010000000
VERSION     DAOPMW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 34239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34239
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_34239_cov_10.354991"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1138)
                     /locus_tag="PLU11_00290"
     CDS             complement(20..1138)
                     /locus_tag="PLU11_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPN57568.1"
                     /translation="MKGYLTAVLIILTGFNAYTQGKLIDKKATAETKALARNLEKLSR
                     KHILFGHQHATEYGHGWSGDLDRSDVKSVTGSHPAVIGVDFSGLSGRPQVEIEKEKDR
                     LRKIIEQAYNRGGVVTVSWHFINPVSPGGFYWVDTVSKPAVKFIIPGGSFHDGYKSIL
                     KTIAELADSCKGNDGKRVPMIFRPFHELDGYWFWWGKNYCTTDEFKSLWRFTVSYLRD
                     TLQVRNFIYAFSPDCTFNSEAEFAERYPGNEWVDMLGFDDYADFGRYGKYNLDSAFAR
                     LKIMTGFAKKNHKLAALTETGLESIPDTTWWTGKLMATIRRAKMRLSYVLVWRNDQHS
                     PTHYYAPYPGQVSVNDFLFFYNDPYTLFEKDLKKIYKR"
     gene            complement(1144..3129)
                     /locus_tag="PLU11_00295"
     CDS             complement(1144..3129)
                     /locus_tag="PLU11_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPN57569.1"
                     /translation="MKKLVLTLLLTVTLFQLIAQKTKTYQLFSPDKKNKITVVAGVRI
                     SWSVQHGQVTVLDPSTLAIEQTGWAIIAGVSPVLRARQQVINDTFQTIAYKKDRVINH
                     GNELILDCKGDYSIVFRAYNDGVAYRFVTRFPGETIVAREEAVFNFPGDPLVHIPFTS
                     DLRQQERYSCSFEEFYTSSKLSVLPPDSLAYLPLMVNLDDTLKAVLLEADVQNYPAMF
                     VQKNAISPNSLNAVHAPYPAAEATRGKYGINYAVTKRENFIARTAGTRSYPWRVIVIS
                     EQDKELLDNDMVQRLSAPSMIADPSWIKPGKVAWDWWNDWNLSGVDFKAGINTPTYKY
                     YIDFAAANKLEYVVLDEGWSDDWDLNKLNERIDLKELLAYAKQKEVGLILWSTWYALL
                     QDIDGLCAKYAAMGVKGFKVDFLDRNDQKTVASCYEIAAIAAKHHLLLDFHGMFAPQG
                     LQRTWPNVVNFEGVRGMEYMKWKPDDRVQEHALSIPFIRMMAGPMDYTPGAFRNMTKG
                     NARPSNSLPQGLGTRSQQMAMYIVYEAPLQMLADNPTIYTKEQECTDFIAAVPTSFNE
                     TKALDAQFGEYLVMARRKNDTWFVGGMTNWTARDITLDFSFLPEGAYTAVLFRDGINA
                     DRDATDYKKEIQTVSNSRKLIIHLAPGGGFAMRLIKK"
     gene            complement(3139..4122)
                     /locus_tag="PLU11_00300"
     CDS             complement(3139..4122)
                     /locus_tag="PLU11_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN57570.1"
                     /translation="MKYNRTGKSGLLLPAISLGLWQNFGNADQYDSMKAIVLGAFDRG
                     VTHFDLANNYGPPGGEAERNFGKMLKEELYAYRDELIISTKAGYYMWPGPYGEWGSRK
                     NLIASCDQSLKRIGLEYVDIFYSHRPDPNTPVEETMQALDSIVRSGKALYAGISNYNA
                     EQTRKAASVLNQLGTPCLIHQPRYSMLDRWVEAPGTTDGKSLLDVVEETGMGSIVFSP
                     LAQGRLTDKYINGVPTNSRIGRALPNGAIQTKDLSADMIEIIKQLNHLALERGQTLAQ
                     MAIAWLLKDHRVTSVLVGASSVAQLQNSIDALNTAAFNTDELQKIETILSH"
     gene            complement(4209..5285)
                     /locus_tag="PLU11_00305"
     CDS             complement(4209..5285)
                     /locus_tag="PLU11_00305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN57571.1"
                     /translation="MVKSFRYLLGFVLFFLLTERAGAQVSLKFYPADHSSIQYMGRID
                     FSNPKLPRFWQPGVTISFCFKGTDAEIILNDEQLWGSNQNYLELVVDGNAVRLQTKGK
                     RDTLSVKPYLKAGNVHEVVLCKNTEANIGYLELAGIRCDQLLKPVKQPTRKIECIGNS
                     ITCGASSDLSGIPCGKGKWQDQHNAWLSYGAITARSLDVQVHLSSVSGIGLMHSCCNL
                     EILMPQVYDKISMRNDTIQWDFNRYQPDIVTVCLGQNDGVQDSTAFCNNYISFLKTLR
                     GYYPNAKILLLSSPMADARLREFLRSSLKSVSGKMKTAGDSNLAMHVFEKSYTGGCDY
                     HPSVEEHALIAKELTEVIRKWMKW"
     gene            complement(5295..7550)
                     /locus_tag="PLU11_00310"
     CDS             complement(5295..7550)
                     /locus_tag="PLU11_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPN57572.1"
                     /translation="MKANILLVIGSFFAFVAKAQKQVPPAEAQAAALVKKMTLEEKVG
                     QMTQVTMAVFAKGAWANEDGELDPVALKKAIVDYKVGSMLNTTAHALPVEKWRSIMTQ
                     IQDEVKNTRLRIPVIYGLDAIHGQTYTLNSTLFPQNLAIAAARNPELVRQAAKVTAME
                     LRSSGVRWNFAPVLDIGRQPLWSRFPETYGEDVYLVKTLGVAGIKAYEEDGLKNPTAV
                     ATSLKHYLGYSASRNGKDRTPIYLPEIELREYYLPQFREAVRTGSASVMVNSSEINGI
                     PVHANRYLLIDVLRKELGFEGVIVTDWEDIKRLHDRHNVAATPREAVVMAINAGIDMS
                     MVPSDYSFYDLLIEAVKKGEVPMSRIDDAVKRILVMKYKTGLFENPYPEPASFGNFGK
                     PEFQQYALEAAREAMTLLKNSDNILPLAKNKKVLVAGPAAQSISALNGCWSYTWQGND
                     DRWYPADSKTIYQAFVDKIGAGNVLTTTQKGFGSPQNYDVNALKTAATLADVIVLCIG
                     ENAYAESPGNTRDLALPKEQEDLVVAAALTGKPVILVLTEGRPRFITNIEPAAKGILM
                     AYWSGRKSAEAIADVLFGDYNPSGRLPFSYHKSMGEIVLYDRKPTEEVREVFNDNINT
                     GYDPLYAFGHGLSYTSFSYSGLTLSSPTLNATGSLTVTLQVKNTGNRAGKHSVELYTR
                     DLYASITPNMRRLRAFQKISLQPGEQKEVRFTLTKEDLAFVNAQLKTVTEPGEFEVFV
                     GELKAKFMYQQ"
     gene            complement(7582..8193)
                     /locus_tag="PLU11_00315"
     CDS             complement(7582..8193)
                     /locus_tag="PLU11_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57573.1"
                     /translation="MGNGNPVSLEPERFIPSVHAVQPYLDKEKSLMEFPDSKAMLEPD
                     FDDAIWEPAFKADRDSLFGRQAKQVISRGHFVLPETDSTTVFTYFSRNIGKAQSVYIN
                     GVRIAAALNADSVIRLSRSLIRPGKNSFVIVAMPFLKVFPWDEVNKDPGMIQLYTPAG
                     KWQRKLFNGYAQVLVQSTGQQGRIILRASAAGLPVAQLEIEAN"
     gene            complement(8199..10433)
                     /gene="galA"
                     /locus_tag="PLU11_00320"
     CDS             complement(8199..10433)
                     /gene="galA"
                     /locus_tag="PLU11_00320"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HPN57574.1"
                     /translation="MKGWLLIGCLWLAGFTGLIAQQQTLVPRTRILMDTGWRFAFGHP
                     WDAAKDFNHATSYFSYLAKASYGDGAAGTRFDDRAWRMLDLPHDWAVELPFDSKGSLS
                     HGFKALGRNFPENSVGWYRKHFYIPKADEGKRIAIQFDGVFRQCSVWVNGFYLGTEPS
                     GFTGMEYNLSEYLNYGGENIIAVRVDATMEEGWYYEGAGIYRHVWLQKTDPLHVITHG
                     SFVNPIPEGNDARIRVSTSLINEQLKEAGCTVRYRVIDATGNNVSEAAIRDFNLAPWQ
                     QKDIQSELKLAGAHRWSLDTPYLYTLVTEIRNGETIKDRIQTVFGIRSLRFDPDKGFF
                     LNDKPIKIKGTNNHQDHAGVGTAVPDALMGYRLRQLMKMGANAYRTSHHPPAPELLDS
                     CDRLGILVIDENRLMGVTDHHFDYLKRLILRDRNHPSVISWSIGNEEWGIEGNELGAK
                     IAQSMQAYVKTIDTTRAITAAISGGWQRGISNVIDVMGVNYVGQINTDQHHQEFPKQP
                     MWGTEEGSTRATRGVYVKDDAGHLLPAYDARPYPHFKSIEEGWKYYMARDYLGGMFIW
                     TGFDYRGEPTPYGWPTVISYHGMLDLCGFPKDVTGYLRSWWTKDTVLHLLPHWNWKGE
                     EGKVKDVWVYSNCTEVELLLNGKSLGKKKMEPLGHLEWKVPYKPGTLQAIGYINGKKI
                     RETKRETAGEASKAVLSADRNLIRADGMDLSVITVAVADAGGKVLPTAANGFTFELEG
                     VPDG"
     gene            complement(10470..13328)
                     /locus_tag="PLU11_00325"
     CDS             complement(10470..13328)
                     /locus_tag="PLU11_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPN57575.1"
                     /translation="MKKIFIAAFLLFISSLTVFAQKPVIIKTGEGVIVYPYQPREGSV
                     KAVRLKVFADGIIRVTSAPELSFNDKESLTVIKQPEKTGWEMEEQNGYVLLKTKELVA
                     KIKLSSGAVSFFDQSGKTLLRERDINGKSLQPVMEEGKKLYSLRQVFESDKEEAYYGL
                     GQHQDDSWNYKGKKVIFFQNNTEVAVPYLVSVKNYGILWDNYSYSEAGDVRPMQQLSA
                     LALYDKKGQPGWLTTSYYNDKDKPGDLVFEKAESEIWYPYMNDLLQRAPKEFKPATGK
                     IVWEGSLSADQSGYYAFRFTYGGYLKLSVNGKVVFDKWRQSWNPGTVLADIFIEKSKR
                     SEVKIEWIPDGGESYVSASFLPPAPLEDEHTFSFISSAGSQIDYYFVKGNSPDEVVKG
                     YRELTGKAPIVPRWAMGFWQSRERYKSQEDILNTVAEFRKRKIPMDNIVQDWFYWKEN
                     DWGSQDFDRSRFPNADSMIEVLHKKYNAQLMISVWPKVYENIPVYNEFNKNGWLYTRN
                     IADRQKDWVGPGYVSTFYDVFNEKARKAFWELLNKKLYSKKIDAWWMDASEPDIQSNV
                     SPEKRKQQMQPLAMGTAAEFMNAYPLLNAKGIYEGQRGADSTKRVFLLTRSAFAGSQR
                     YAASIWSGDIGARWEDMRTQISAGLNYSISGLPYWTLDIGGFATEVRYNKVPMAPADE
                     EEWRELQTRWYQWGAFLPLFRSHGQFPVREVFNIAPEDHPAYKSMLFYNQLRYRLLPY
                     NYSLAGHAYHHDYTLLRGLVMDFAADKKTWNIGDQFMFGPSLLVAPVYTYKARTRTVY
                     LPAGQGWYDLYSGKFYKGGQTITSEAAYERTPVFVKAGSVIPTGPALQYTSEKAADPL
                     TLNIYTGKDASFTLYEDEGVNYNYEKGKYSEIPITYCQSANTLTIGDRTGSFAGMLNS
                     RTIYIKVVSAVQAMPFAFDGKETGRKIEYKGKKIVIRL"
     gene            complement(13335..15071)
                     /locus_tag="PLU11_00330"
     CDS             complement(13335..15071)
                     /locus_tag="PLU11_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HPN57576.1"
                     /translation="MTITSRFLYLSVLISLPFFILSCKKKGGTTPPPVGDTTTVNPQV
                     DPPLANTIGFFMDDWQPRTFTVPTTFTNTTVPAAAGVTVTIDRSNVLTKVPRSYASAN
                     ANLWMGQMVTETSLMTNITNLHPHLIRFPGGSISDVFFWNAQPNTPPADAPAQLIQDN
                     GTAVAAGFWYGKNTDNRTFSVDNYYSMLQQTGNKGMITINYGYARYGTGANPVARAAH
                     LAADWVRYDNGRTQYWEIGNENFGNWEAGYRINTATNQDGQPEFVTGQLYGQHFKVYA
                     DSMRKAAQEIGKTIYIGAVMSESAPLSWWTPTATTWNSGLFAAAGNYPDFYIVHNYYT
                     PFQTNATAEVILNTPVEETQKMMDYVRQTISGAGLTQKPVILGEWNITSSGSKQQVSH
                     VNGLHAVMVLGESLKNKIGMTARWDLANGWSNGDDHGMFNQGDEPDGVPKWNPRPAFY
                     HMYFFQQYLGDRLVAANSTNADVISYGSSFTSGETGVAIINKAADPKSVQVDIKNFKK
                     GSRFYWYTLTGGTDNGEFSRKTLVNGITSASAAGGPSTYKELSPYSGSTTNGIRVVVP
                     GRAAVFMVVSKQ"
     gene            complement(15081..16283)
                     /locus_tag="PLU11_00335"
     CDS             complement(15081..16283)
                     /locus_tag="PLU11_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPN57577.1"
                     /translation="MEHINPGARNYFLQELRSELEAILNYWIKYVPDNQQGGFYGKVD
                     NDNIPDKTAAVGSVMCSRILWAFSAGTAITGNPAHQEMADRVYAYINDFFIDRQYGGV
                     YWSLKPGGEALDTKKQLYGQAFCIYGLSAYYQLTGHEAALQTAIELYRCIEQYGFDNE
                     KNGYIEAFARDWSAIGDLRLSAKDNNERKTMNTHLHVIEAYASLYSVWPDPSLKERIT
                     NLLGLFEQYFVDHFHYHLRLFFDDDWHSKSARRSFGHDIEAAWLLQQCAEIIEDKALT
                     ELFRKLAIPITNAAAEGQDKDGGLWYEFEPAENKLIREKHSWPQAEALVGFYNAWQLT
                     GEKKYYQHCLDTWAFIRQYIRDHEKGEWFWGIYEDYSIMNEDKAGFWKCPYHNSRAML
                     ELMKRINE"
     gene            complement(16292..17515)
                     /locus_tag="PLU11_00340"
     CDS             complement(16292..17515)
                     /locus_tag="PLU11_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPN57578.1"
                     /translation="MRNNFYSRLARLEKTHEELVARQNEREAAENGNGIVDRYKYPVL
                     TAAHTPVFWRYDLNPDSNPYLLQRFGINAVLNPGAIKWNDKYLLVARVEGADRKSFFA
                     VAESPNGIDQFKFWDYPLQMPETDEPDTNIYDMRLIAHEDGWIYGVFCTERRDPSAPA
                     TDQSAAIAQCGLARTKDMKNWERLPDLKTQSPQQRNVVLHPEFYNGRYAFYTRPQDKF
                     IEAGKGGGIGFGVCDCMEGAVIDEEQVIDPKVYHTVYEMKNGLGPAPIKTAQGWLHLA
                     HGVRNTAAGLRYVLYMFMTDLHDISKVIYKPAGYFMAPEGEERIGDVSNVVFSNGWIV
                     EEDGRVLIYYASSDTRIHVATSTVDKLLDYVMNTSPDEFTSAESVKTLTELIDRNLSL
                     PVEKEEGTANAAFAG"
     gene            complement(17535..19364)
                     /locus_tag="PLU11_00345"
     CDS             complement(17535..19364)
                     /locus_tag="PLU11_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPN57579.1"
                     /translation="MKLTLIDISIIVSYLITMLMLGWFLRKKARVNKESYLMGGKKLP
                     WYMLGMSDASDMFDISGTMWMVALCFVYGIKSIWIPWLWPVFNQVFNMMYLSKWLRRS
                     NANTGAEWLATRFGLKGAGVKGSHAIVVAFALIGCLGFLAYGFIGLGKFIEIFVPWEL
                     VQDYIPFDIAPQYVAHFYGIIFTLFAMFYSILGGMHSIVLGDLIKYAIMTIGCIAIAA
                     IAMIHLRGNTLNVPAGWSDPFFGWNLNMDWSGIVNEANEKIKEDGYGLFGLLFMMMLF
                     KGVFAAAAGPAPNYDMQKILSTKSPKEASKMTGFVSIILLPVRYAMVIGLTVLGLLYY
                     NQLNLSNGNGGVDFERILPGAINSFLPVGILGLVLTGLLGAFMGTFSGTLNAAQAYIV
                     NDIYLKYVNKDASNKKIIRMNYLVGVLVVALGVILGFFAKDVNSILQWIVSGLYGGYI
                     ASNVLKWYWWRFNANGFYIGMLTGVTGALVFSRLFTGIEFLYYFPVLFLLSVAGSLIG
                     TFTAPPTEAETLKKFYRTVRPWGFWKPVHELVVKEEPGFEPNRRFKLDMFNVVLGIIA
                     QLCMTVLPMYVVLSMHLPLLITILILTLIILILKKTWWDKLED"
     gene            complement(19479..20618)
                     /locus_tag="PLU11_00350"
     CDS             complement(19479..20618)
                     /locus_tag="PLU11_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPN57580.1"
                     /translation="MKTVRLLVITVFCLLAVYTVWAQTGPVDPSATKETIGLYKNLLT
                     LQDKGFLFGHQDDLAYGVEWRYKEGRSDVKEAAGDYPAVYGWELGGIERPGNDKNIDG
                     VLFKKMKQFIRQGYERGGVITLSWHADSPLGGGKGAWDTTHGSVSSINPGGANHAMYK
                     EWLDKVAAFIGSLKGSKGEAIPVLFRPFHEHSGNWFWWCRNACTVFEYQTLWRFTYYY
                     LQTEKKLHNLLWVYNTGGDFNTKEEFLERYPGDDKVDLLSFDTYQYGDPEKEKWFEEN
                     TNRRLKMVGELAAEKGKPFALAETGYEAVPYAAWWTQTLLNAIGDNKISYVMVWRNHG
                     YQAETKKMHYYAPYKGQVSEADFRKFAALERVMFEKEAFNEQLYQ"
     gene            complement(20615..21898)
                     /locus_tag="PLU11_00355"
     CDS             complement(20615..21898)
                     /locus_tag="PLU11_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPN57581.1"
                     /translation="MRLLVLTVALTAFLQAMAGDPFVTVQNGRFMLGQKTYRYIGVNY
                     WYGGLEARTTKGKQRIKEEVAFLAAKGVRNVRVLAGAEGAGPINGVPRVEPALQTAPG
                     VFNIQLLEGLDFLLAELAKKKMKAIIYFSNNWEWSGGFLQYLNWNGKIADSVLKRKLS
                     WDEMRDLASGFYDCADCVSQYTTQLKLLVERTNSITKKKYRDDPAIMSWEIANEPRPM
                     RPAAIDAYKKFIRETAAFIRSLDPNHLVTTGAEGDIGSENMDVFRSTHEDKNIDYLTI
                     HIWPKNWGWFSDTAIGRSFDQVLEKTGAYINRHKEVAVALKKPLVIEEFGLPRDQHSF
                     SLQSSTIYRDLYYAFVFDYLLDAEKNAGVICGANFWAFAGKGRPSHRQLLWVEGDDVL
                     GDPPVEEQGLNSVFDADKSTWNVITSCIKQLNQFK"
     gene            complement(22028..23068)
                     /locus_tag="PLU11_00360"
     CDS             complement(22028..23068)
                     /locus_tag="PLU11_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPN57582.1"
                     /translation="MKQFIKNQLSIFLLMLGLGALFITGCQKDSDGNPEVSTGGMSSG
                     ELNPGSAAGGEVITLKGSGIGQVRSIVFDKNNVPAPFLSTLNTTTDLVFRVPDTAFGG
                     PQNVIFTNADGKTLTVPFSVIALPNVTAAFPTDFEAGTQVTITGNNLDDVSAVVLEGT
                     TDACTIVSQTRKQIVVTMPASTANRAKLKLTNASGIRITDMEFVNVGQALTVFTEQLN
                     NGFENWGWGGDYNASTDDKITGAKCLKAAYDPAGSWGGMQLGNGGSINIAGYRYFSFW
                     AKGADVDKNVQFRINWGNQKVVTIPANKWTYFRYDLTTAYPGLSNDVNNVTFQIQEAG
                     KTLLFDNIMFFK"
     gene            complement(23104..24672)
                     /locus_tag="PLU11_00365"
     CDS             complement(23104..24672)
                     /locus_tag="PLU11_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN57583.1"
                     /translation="MKNILRYTAIVLLPVVALFSCKKEFLDKPALDSTTLDNYYNTAE
                     EARGLTSTLYGLPWAGYENRAMDAIGDVMAGNEYTGGGDDPPFINFSFASTSVRIADA
                     WKVFYKIGGWTSEYMNALELKKSKGGDASFLDPAIAECNFFKGTVYFYIARIWGDAPI
                     VNNPGATILSGDFNIPRYFKADVLRFALEELKKAEAGLPETDVPGRVTKYSAKGMMAK
                     LYLYRKDYDSAKMKALEVINSGKYDLFNDYGAMFNSSANNNNIESLFSIQHQLTGNPW
                     GSGNQKNPDRGPGNLQTSEASMWELYTPSRDMINAYESGDIRRKGSMMEHGWTNPQWK
                     PKNANATYNAFMAGGYKYDTLQPVGDGGQKNQVRANIAKYVVGPGSAFGGEAVLGVNT
                     GINTMMLRYADILLIYAEAVLGTNASTTDASALAAFNKVRARAGLLQKTSLTPDDILK
                     ERRVEFAFEGDYWFDIQRQGFAKAKQIIEAQNRGTFDSPMYVTFTQNSMHLPIPAGEV
                     LQDPALAQPPVAYY"
     gene            complement(24700..27858)
                     /locus_tag="PLU11_00370"
     CDS             complement(24700..27858)
                     /locus_tag="PLU11_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57584.1"
                     /translation="MRKTRFPRAITMIIVLVLYSLTVFSQNRVISGVVSDQSGVPLVN
                     ATVSVKGSKTTVITDNYGAFKITVPPNATALVVSYVGAKPKEVSLEGKTNILVSLDVT
                     DTKLSEVVVIGYGTAKRSDVSSAISSVKTKDLKDLPVAGIDQALQGKVAGVTVVNNSG
                     QPGGGVSLRIRGVTTINANDPLIVVDGVIFASNTKFSAGYAGLGGSDGQTGNSFLATI
                     NPNDIESIDILKDASAQAIYGSQAANGVILITTKKGKSGEGKINYEMYAGNQIVQKKL
                     DLMNLREFAQYQNEVSPLLGFTPSAEFADPSILGRGTDWQDAMFRTGTVQNHQLSFSG
                     GKDKTNYYLSANYFTQKGILIGSDFKRYSTRFSLDHQLKNWIKVGVSANASRSIQNVT
                     LADAAEGTIWWGTLQSPLIPVKNLDGTWGGGGQTIGGFQYAQDNPIARSFYRGNKSTS
                     SQVFGQLYAEIEFMKGLSLRNEFAYSLSLYNNMAFQRTGNFGNTSLRSQLFEYRGNGY
                     YYAARNYLNYYKNIGKHAISATAGHEAQYNYWESLNGKKVDLQNNILDLNAGSSDRDT
                     WELGGGKNDGTIESYFARGTYTYDNRYALNLSIRRDGSSRFFGKNKWGNFPGASFAWT
                     VTNEKFAENMKKTINSLKVRIGYGEVGNTNMPNGAPNPPYTSVVSFWPGPVGFGSSNY
                     LYGVANPDLTWESVITKNAGVDISFLNGRIETAIDVYKKVTSQMLLIGTGPRLIGVGD
                     RWDDLKGPVINTGRMSNTGIDFSLTSTNIRNKDLTWKTNIIFSQFKNKLEKVAGDLAA
                     LYGRIYYDNYLISRTIPGFAVGSFFGLKTDGLYRTQDDLNNSLPQFGYSVDQTHTWLG
                     DVRYKDVGGADGKPDGVIDEKDITFIGSPLPKFTYGFTNTVNYKNFDFSVFIQGSHGA
                     KIFNFLRWQLEKMDNPYYNQLRSVMDRYTDANTNGKLPRFTNTNVNNVYMSDRYVEDG
                     SYLRIQNIAIGYRIPAKYLSKAKISTARVYITVQNLHTFTNYSGYDPEVGVFNNNIRL
                     TNVDAGHYPNPRSFTAGINLEL"
     gene            28075..28953
                     /locus_tag="PLU11_00375"
     CDS             28075..28953
                     /locus_tag="PLU11_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN57585.1"
                     /translation="MHKNILREITPLTPSDCFTIFTRVKTDFDFPLHYHEELELNFIH
                     NAKGARRVVGDHIAEIDDLELVLVGSNLPHVWQTHKCKGRKITEVTIQFHKDLFDEKF
                     LRRNQMSFIRNLLERSAKGILFSRTTAEQLKSRLLALEQKQGFDSVLELVSILHDLSV
                     SRNMHTLSDATFSNAELSYNSRRIERAFEYMNENFHKNITLTEVSKLVNMTDVSFSRF
                     FKAKTGITFIDSLLELRLGHASRLLIDTTESVSEIAYDCGFNNISNFNRLFKKKKNCT
                     PKEFRESYSTDNRVFI"
     gene            29132..29770
                     /locus_tag="PLU11_00380"
     CDS             29132..29770
                     /locus_tag="PLU11_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57586.1"
                     /translation="MYFQEAIAPYAGQPLTQQLLLDILREYKRPYDKITELVKQEQLI
                     QVKRGLYIPGAKLKLPPPVSFLLANHLYGPSYVSLDSALSFHGLIPERVFEISSMTLR
                     AAKTYDTNAGRFSYTKMPQPYYSYGIQSAELATNQVALVATPEKALCDRIINTSQLQL
                     RSVKQTIDFLTEDLRIEKSSLRQLDAGAISRWIKIAPKKTSLAMLVKTLNSL"
     gene            29767..30618
                     /locus_tag="PLU11_00385"
     CDS             29767..30618
                     /locus_tag="PLU11_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPN57587.1"
                     /translation="MIKEWLETYKPAHTEDALQALREIMQEIALAGLQRSGFFEVAAF
                     YGGTALRIFHGLPRFSEDLDFSLLEKNNDFSLAPYLDGMENEFAALGIKVSVREKSKT
                     TITAFDSAFLKPDTTWKELIIKAVLPDERIKLTPEIKIKLEVDTMPPPGFDTEEKLAL
                     KPFSFYTKCFTLPDLFAGKMHALLFRKWKGRVKGRDWFDLEWYIQKGVPLHLDHFRQR
                     AIDSGDWTKKTMTEKQFNQLLTDKIKAISFKAVKEDIIRFIPDAKVLEIWSAEYFNDL
                     SEKIKFG"
     gene            complement(30627..30989)
                     /locus_tag="PLU11_00390"
     CDS             complement(30627..30989)
                     /locus_tag="PLU11_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57588.1"
                     /translation="MKKWVVLDTTERKALLSEHFQKYLKEQTDFLKENNRLMNEAEKE
                     EWTKRFMQHPYNHLNVRVFIRTTKTNVVTDSIKWQVRIPRMDTIQYKLFIPAEERKEI
                     YASLKDFADTVLVSGLLR"
     gene            complement(31288..>34239)
                     /locus_tag="PLU11_00395"
     CDS             complement(31288..>34239)
                     /locus_tag="PLU11_00395"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPN57589.1"
                     /translation="YESYGIAGKPQGHYYVDAVDGVLKKSQNAYERPQPHACFILSVD
                     DDLVNEGGIMDLWVREARIFKYGSGVGTNYSNLRGEGEKLSGGGSSSGLMSFLKIGDR
                     AAGAIKSGGTTRRAAKMVCLDLDHPEIVEFINWKVEEEKKVGALINAGYPSGYEDEAY
                     KTVSGQNSNNSIRIPNSFFEKLEKGEDWELTGRMDGRVMKKVPSRELWNQIAYAAWRC
                     ADPGTQYNTTINEWHTCPEGGEIRASNPCSEYMFLDNTACNLASANLMKFYDADNNQF
                     DVEGYEYNCRLWTVVLEVSVLMAQFPSKEVAQLSYEYRTLGLGYANLGTMLMVSGIPY
                     DSEEARAIAGAITAIMTGTSYKTSAEMARALGAFPRYEENKAHMMKVMRNHRLAAYDA
                     DEYEQLSLKPQGIKVKYCPDYLLKAACSAWDDAVEMGEKYGYRNAQATVIAPTGTIGL
                     VMDCDTTGVEPDFALVKFKKLSGGGYFKIINQSVPTALRNLGYSEKETDNIIKYAVGA
                     ASFAGAPFVNHQSLSEKGFIADEIKRLDAAAATAFEIGFVFNKYTLGEECLQRLGFTP
                     EQYNNFEWSLLEALGYTDEQIEAANDYVCGTMMLEGAPGLKDEHLPVFDCANKCGQKG
                     ERYIHAHGHIRMMGATQPFISGAISKTINLPHEAKVEEIADAYHLSWKLGLKANALYR
                     DGSKLSQPLSNKSDKKKKDTAVEETKAAVVEQAPVAESNIVDLGKLTVEELLEEVQKR
                     VQASPDTKLKRKLASIVERRTLPAKRRGFTQKAKISGQAVFLRTGEYADGTLGEIFVD
                     MAKEGATMRSMMNCFAISVSIGLQYGVPLEEFVDKFAFTRFDPSGFVEHPNIKTTTSI
                     VDFIFRVLGYEYLGRTDLVHVLDKPEVMNTGGDDWDEVPSNPLEYVKEPELSSVRVTP
                     SSGSVAPQPAKAQQRAAQPVKSESSMDAMNAVAKNMQSDAPACNVCGNIMMRSGACYK
                     CLNCGNQGGCG"
BASE COUNT         8784 a         8596 c         7416 g         9443 t
ORIGIN      
        1 gttaacagga taattttttt catcgcttat aaattttctt caggtccttt tcaaacaacg
       61 tataaggatc attataaaaa aacaggaagt catttacaga tacctgcccg ggatagggag
      121 catagtagtg agtggggctg tgctgatcat tgcgccatac gagtacatag gaaagccgca
      181 ttttcgctct tcgtattgta gccatgagtt tgccggtcca ccaggtcgta tcgggaatgg
      241 attcaagtcc ggtttctgtt aatgcggcca gtttatgatt cttctttgca aatccggtca
      301 tgatctttaa ccgggcgaag gctgaatcaa gattatattt cccgtaacgg ccaaagtcgg
      361 cataatcatc gaatcccagc atatccaccc actcattacc cgggtaacgt tccgcaaatt
      421 ccgcctcgct gttaaaggta caatcaggag agaaggcata gataaaattc cgtacctgta
      481 acgtatcccg caggtaggat acggtaaaac gccagagtga tttaaattca tccgtagtac
      541 agtaattttt accccaccag aaccagtatc cgtccagctc atgaaacggc cggaagatca
      601 tgggcacccg ctttccatca ttccccttgc atgaatcagc cagctcagca attgttttta
      661 aaatggattt atagccatca tgaaaagagc cgccgggtat tataaacttt acggcaggct
      721 tggaaacagt atccacccaa tagaaaccac cgggggaaac agggttgata aaatgccagg
      781 aaactgtcac cacccctccc ctgttatacg cttgttcaat gatttttcgc aaccgatcct
      841 tttctttttc aatttcgact tgcggtctgc ctgaaagccc actgaaatcc accccgatca
      901 cagccggatg tgaaccagtt actgatttca catcactccg atccagatct ccgctccagc
      961 catgcccata ttcagtagca tgctgatggc caaaaagaat atgttttcgg gatagttttt
     1021 ccaggttgcg ggccagggcc tttgtttcag ccgtggcttt tttatctatc agttttccct
     1081 gcgtataagc attgaaacca gtcaggatga taagaacagc tgttaagtac cccttcatta
     1141 tcgttatttt tttataagcc gcatagcaaa accaccaccg ggtgccaggt gaatgattag
     1201 tttacggcta ttgcttactg tctgaatttc ttttttataa tcggtcgcat cccggtcggc
     1261 attgatcccg tcacggaata aaacagccgt atacgctcct tccggcagga aagaaaagtc
     1321 cagcgtgata tcacgtgctg tccagttggt cataccaccc acaaaccagg tatcattctt
     1381 ccggcgggcc ataaccaggt attccccgaa ttgtgcgtcg agtgcttttg tttcattaaa
     1441 actggttggc accgcagcaa taaaatcagt gcattcctgc tcttttgtat aaatagtcgg
     1501 gttgtcggcc agcatctgga gtggggcttc gtatacgata tacatagcca tttgctggct
     1561 gcgggtaccc aggccttgcg ggagactgtt actgggtcgg gcattgccct tggtcatatt
     1621 ccggaatgcg ccgggtgtat aatccatcgg accggccatc atacgtatga aaggtatact
     1681 gagtgcatgt tcctgtaccc ggtcatctgg cttccacttc atatattcca taccccgaac
     1741 tccttcaaaa ttgaccacat tgggccaggt gcgttgcagt ccctgagggg caaacatgcc
     1801 atgaaaatcg agtaacaagt gatgtttggc agcaatcgcc gctatttcat aacaggaggc
     1861 caccgttttc tgatcattgc ggtccaggaa gtctacttta aatcctttca cacccatggc
     1921 cgcatatttg gcacaaagac catcgatatc ctgaagtaac gcataccagg tgctccagag
     1981 gatcagccct acttcctttt gttttgcata agcgagtagt tctttcaggt caatccgctc
     2041 attcagttta ttcaaatccc agtcatcact ccagccttca tccagtacta catactctaa
     2101 cttattggct gcagcaaaat caatataata tttgtaggtg ggtgtattga tcccggcttt
     2161 aaaatccacg ccggaaagat tccagtcgtt ccaccagtcc caggctactt tcccgggttt
     2221 gatccaggag gggtctgcaa tcattgatgg ggcagacaac ctttgcacca tatcattatc
     2281 gagtaattcc ttatcctgct cactgatcac gatcacccgc cagggatagg aacgggtgcc
     2341 ggcggttcgg gcaataaaat tttcgcgttt ggtaacagca tagttgatcc catacttccc
     2401 gcgtgtagct tctgctgccg ggtaaggagc atgcacggcg ttgaggctgt tgggggatat
     2461 tgcattcttc tgcacaaaca tggccgggta attttgaaca tccgcctcca gcaaaacagc
     2521 tttcagggtg tcatccagat ttaccattaa tggcaggtaa gccagcgaat cgggtggcaa
     2581 tactgataat ttagacgaag tataaaactc ttcaaaggaa caggaatatc gttcctgctg
     2641 ccgtagatca ctggtgaatg gaatatgcac caggggatca ccgggaaaat taaatacagc
     2701 ctcctcgcgc gccactatgg tttcgccagg gaaacgcgta acaaagcggt aagccacccc
     2761 atcattgtac gcccggaaaa caatactata atcaccttta cagtccagta tcaattcgtt
     2821 tccgtgattg atgaccctgt cttttttata ggcaattgtc tgaaatgtat cattgatcac
     2881 ctgttggcgt gcacgtagta cagggctcac tccggcaata atggcccatc cggtctgttc
     2941 aatagccaat gtggatgggt caagtacagt tacctgacca tgctgcacgg accatgaaat
     3001 gcggacaccg gcaacaacgg tgatcttatt ctttttatcc ggtgaaaaaa gctggtaggt
     3061 ttttgttttc tgtgcaatca actgaaacaa agtcacggtt aacaataaag taagtaccag
     3121 ttttttcata tgggttgttt aatgtgagag tatggtttcg attttctgta attcgtctgt
     3181 attaaatgcc gctgtgttca gggcatcaat actgttttgt aactgtgcta cagagctggc
     3241 ccccaccaga accgaagtaa cccggtggtc tttgagcagc caggcaatgg ccatctgtgc
     3301 cagggtttgt ccacgttcca gggccaggtg attgagttgc tttatgatct caatcatatc
     3361 cgcgctcagg tccttggtct gaatggcgcc attgggtaat gcccgaccga ttcttgaatt
     3421 cgtgggcacg ccattaatat atttatcggt cagccggccc tgtgccagcg gagaaaacac
     3481 aatactcccc atcccggttt cttccaccac atccagcagc gatttaccgt cagtggtacc
     3541 cggggcttct acccaacgat ccagcatgga atagcggggc tgatgaataa gacagggagt
     3601 tcccaattgg tttaatacag acgctgcttt acgggtctgt tccgcattgt aattagaaat
     3661 accggcataa agggctttcc cactacgtac aatactatcc agcgcctgca tagtttcttc
     3721 caccggtgta ttggggtccg gacgatggga ataaaaaata tccacgtact ccagtcctat
     3781 ccgcttcagg ctctgatcac aactggcgat cagatttttc cgggagcccc attctccata
     3841 aggtcccggc cacatataat aaccagcctt ggtggaaata atcagttcat cccggtaggc
     3901 atacagctct tctttgagca ttttaccaaa attgcgctcc gcttcgcccc cgggaggtcc
     3961 ataattattc gccaggtcaa aatgggtgac accccggtca aaagccccga gaacgatcgc
     4021 cttcatactg tcatactgat ccgcattccc gaaattctgc cagaggccga gggagatggc
     4081 aggaagcaat aaaccgcttt ttccggtgcg gttatatttc atggtggcat atcggttgtc
     4141 ggaggcagta tacataaaat aatttttcag caatctacat catcggtggc aacttttcat
     4201 ggaacggttc accatttcat ccacttcctt atcacctccg tcagttcctt tgcaatcagg
     4261 gcatgttctt ccaccgatgg atgataatca catccgccgg tataggattt ttcaaataca
     4321 tgcatagcca ggttgctatc accggcggtc ttcattttac ctgagactga tttcaggctg
     4381 ctgcgaagaa attcacgaag tctggcatca gccatcggac tgctcagcag gagaattttt
     4441 gcatttggat aataccccct caatgttttg agaaatgaaa tatagttgtt gcaaaaagca
     4501 gtggaatcct gaactccatc gttttgtccc aggcaaactg tcacaatatc cggctgatac
     4561 cggttgaaat cccattgaat ggtatcatta cgcatactga tcttatcata aacctgcggc
     4621 atcagaattt ccaggttaca acaactgtgc atcaacccaa ttccggaaac agaggaaaga
     4681 tgcacctgta catctagtga acgtgccgtt attgccccat aactcaacca ggcattgtgc
     4741 tgatcctgcc atttcccttt gccgcaggga atgccactga gatcgctgct cgcaccgcag
     4801 gtaatggagt tgccaataca ttcgattttc cgggtgggtt gcttaaccgg cttcagcagt
     4861 tgatcgcaac ggataccggc cagttcaaga tagccgatat tcgcttccgt gtttttacaa
     4921 agaaccactt catgtacatt gccggctttc agataaggct ttaccgaaag ggtatccctt
     4981 ttccctttcg tctgcaatct gaccgcattc ccatccacca ccagttccag gtaattctgg
     5041 ttgctgcccc agagctgttc atcattgagg attatttccg catcggttcc cttaaaacaa
     5101 aaactgattg ttaccccggg ttgccagaaa cgggggagct ttggattact aaaatcaatc
     5161 cgccccatgt actgaataga agaatgatct gccggataaa atttcagcga aacctgtgcg
     5221 ccggcccttt ctgtcagtaa aaaaaacaac acaaagccca gcaaatagcg gaacgacttc
     5281 accatacaat acatttattg ctgatacatg aattttgctt tcaactcccc gacaaatacc
     5341 tcaaactcac ccggctcagt tactgttttt aattgtgcat tcacaaaagc gaggtcttcc
     5401 tttgttaacg taaaacggac ttctttttgt tccccaggtt ggaggctgat cttttgaaaa
     5461 gcccgcaggc gacgcatgtt tggcgtaatg ctggcataca gatcgcgtgt ataaagctcc
     5521 acgctgtgtt tgcctgcccg gttaccagtg tttttaacct gcagtgttac agtgagtgat
     5581 ccggttgcat tcaatgtggg actgcttaag gtaagtccgg aataactgaa gctggtataa
     5641 ctcaggccat gaccaaacgc atacagggga tcatacccgg tattgatatt gtcgttaaat
     5701 acttcgcgga cctcttcagt cggttttcgg tcatagagca cgatctcacc catgctttta
     5761 tgataagaga aaggcaggcg accggaggga ttataatctc cgaataagac atccgcaatg
     5821 gcttccgctg attttcgtcc gctccagtaa gccatcagaa ttcctttggc agcaggttcg
     5881 atattggtaa taaaccgagg ccttccttct gtgagcacca ggattaccgg ctttccggta
     5941 agtgccgctg ccactaccag gtcttcctgc tctttgggca gggccaggtc gcgggtattg
     6001 cccggacttt ctgcataggc attctccccg atacaaagta caatcacatc cgctaaggtc
     6061 gcagccgttt tcaaggcatt cacatcataa ttctgcggac taccaaatcc cttttgcgtg
     6121 gtggttaaca cattgccggc tccaatctta tccacaaatg cctggtaaat ggttttgctg
     6181 tcagccggat accaacggtc atcattcccc tgccaggtat agctccagca gccattcagg
     6241 gcactgatgc tctgtgcagc aggacccgcc accagtactt ttttattttt ggccaggggc
     6301 aatatgttat cactgttttt cagcagggtc atggcttcgc gggcagcctc cagggcatat
     6361 tgttgaaact cgggtttacc gaaattgcca aagctggccg gttccggata aggattttca
     6421 aacaatccgg ttttgtattt cattaccagg atacgcttca cggcatcatc aatccggctc
     6481 atcggcactt cccctttctt caccgcttcg atcagcagat cataaaagga ataatcagaa
     6541 ggcaccatac tcatatcaat tcctgcattg atggccatga ctaccgcttc acggggtgta
     6601 gccgctacat tatgccggtc gtgcagtcgc ttgatatctt cccagtccgt gacaatcacg
     6661 ccttcaaagc caagctcttt cctcaacaca tcaatcagca gataacggtt agcatgtacc
     6721 ggaattccat tgatctcact ggagttcacc attacagaag cagaaccggt acgtaccgct
     6781 tcacggaatt gcggaagata atattcacgt aattcgattt cgggcagata gatcggtgta
     6841 cgatcctttc cattgcggga agctgaataa ccaaggtaat gtttcagact ggtggctacc
     6901 gcggtcggat ttttcaatcc atcttcttcg taggctttga ttccggcaac accgagtgtt
     6961 ttcaccaggt atacatcttc accataggtt tccgggaatc ggctccacag gggctgacga
     7021 ccaatatcaa gtacgggggc aaaattccag cgcaccccgg atgatcgaag ttccatagcg
     7081 gttactttgg ctgcctgtct gaccaactcc gggttgcggg ctgctgctat agcgaggttc
     7141 tgcggaaaca aagtggagtt caatgtatag gtctgtccat ggatcgcatc cagtccatat
     7201 ataacaggga tccgtaaccg cgtgtttttt acctcatcct gtatctgggt catgatgctc
     7261 cgccattttt ctaccggcaa cgcatgagcc gttgtattga gcatggatcc tactttataa
     7321 tcaacaatcg ctttttttaa tgccaccgga tccagttctc cgtcttcatt ggcccaggca
     7381 cctttggcaa atacggccat ggttacctgt gtcatctgtc ccactttttc ttccagtgtc
     7441 attttcttaa ctaatgctgc agcttgtgct tctgcagggg gtacctgttt ctgtgccttg
     7501 gctacaaagg caaaaaaact gccaatgacc agtaatatat tcgctttcat ataacctgtt
     7561 ttacaaattg attaatccat tttaatttgc ctcaatttcc agctgtgcca ccggcaatcc
     7621 cgctgcactg gccctgagta ttatcctgcc ttgctggccg gtcgattgca caagcacctg
     7681 tgcatatccg ttgaacagct tacgttgcca tttaccggca ggtgtataca actgtatcat
     7741 acccggatcc ttatttactt catcccaggg aaatacttta agaaaaggca tggccacgat
     7801 cacaaagctg ttcttccctg gtcgtatcaa tgaccggctt agccggatga cgctatctgc
     7861 attaagtgct gcagctattc ttactccgtt aatatacacc gattgggctt taccgatatt
     7921 ccggctgaaa taggtgaata ccgttgtgct gtcggtttcc ggtaatacaa aatgaccacg
     7981 gctgattacc tgttttgcct ggcggccaaa tagactatcc ctgtccgcct taaatgcagg
     8041 ctcccagata gcatcatcaa agtcaggctc cagcatggct ttactatcag gaaattccat
     8101 cagtgatttt tctttatcca ggtatggctg taccgcatga acagatggaa taaaacgttc
     8161 cggttccagt gaaaccggat tgccattccc cacaccaatt atccgtccgg gacaccttcg
     8221 agctcaaagg taaaaccatt cgcagctgtc ggaagtactt ttccacctgc atccgcaaca
     8281 gcaacagtta taacggacag gtccattccg tctgcgcgga tcagattccg gtcggccgat
     8341 aaaacagcct tactggcttc gccagcagtt tcccgtttcg tctctcgtat ctttttgcca
     8401 ttgatatatc caattgcctg cagcgtgccg ggcttatatg gtactttcca ctccaggtgt
     8461 cctaatggtt ccattttttt cttcccgaga cttttcccgt tcagcaataa ctccacttct
     8521 gtacaattac tgtacaccca tacatccttt acttttcctt cctctccttt ccagttccag
     8581 tgtggcagca aatgcagcac ggtatctttt gtccaccagc tccggagata gccggtcaca
     8641 tccttaggaa atccgcaaag gtccagcatg ccatgataag aaataacggt aggccatcca
     8701 taaggagtgg gttctccccg gtaatcaaat ccggtccaga taaacatgcc gcccaggtaa
     8761 tcccgggcca tataatattt ccagccttct tctattgatt taaaatgcgg gtaaggcctg
     8821 gcatcatagg cgggtagtaa atggcctgca tcatctttta cataaacacc tcttgttgcc
     8881 cttgtacttc cttcttcggt gccccacatt ggttgctttg gaaattcctg gtgatgctga
     8941 tcggtattga tctgtcccac ataattaacc cccatcacat caatcacatt ggatatgccc
     9001 cgttgccagc ccccgctgat cgctgctgtt atagccctgg ttgtatcaat cgtttttaca
     9061 taggcctgca tgctctgtgc aattttagcg cccagttcat tgccttcaat cccccattct
     9121 tcattgccga tactccagct gatcacagat ggatgattcc ggtcacgcaa gatcagtctt
     9181 ttcaaataat caaaatgatg atccgtcaca cccatcagcc tgttttcgtc gatcaccagt
     9241 attccaagac ggtcgcagct atccagtaat tccggtgcag gcggatgatg ggaagtacga
     9301 taggcgtttg cccccatttt catcagctga cgtaaacggt atcccatcaa tgcatccggg
     9361 acggccgtgc ctacacccgc atgatcctgg tgattgttgg tgcctttaat ttttatgggc
     9421 ttgtcgttca gaaagaagcc tttatccgga tcaaaccgaa gcgaacggat accaaacact
     9481 gtctggatcc ggtcctttat tgtttcacca tttcgtatct ctgtaacgag tgtatacagg
     9541 taaggtgtat ctaacgacca gcgatgggct ccggccagtt ttaattcgga ttgtatgtct
     9601 ttttgttgcc agggtgccag gttaaaatca cggatggcgg cctccgacac gttattgccc
     9661 gttgcatcga tcactctgta ccgcacagta catcctgctt ccttgagctg ttcattgatc
     9721 aacgatgtac tcaccctgat cctggcatcg ttcccttccg gaatcggatt aacaaaactg
     9781 ccatgggtga tcacatgcaa cggatctgtc ttctgcaacc agacatgtcg atagatcccg
     9841 gccccttcat aataccatcc ttcttccata gtggcatcca cccggacagc gattatattc
     9901 tctcctccgt aattcaggta ttcggaaaga ttgtattcca tccctgtaaa tccgctgggc
     9961 tctgtcccca ggtaaaatcc attgacccaa acactgcatt gccggaaaac tccgtcaaac
    10021 tgtattgcta tccgctttcc ttcatccgct tttgggatat aaaaatgttt acgataccaa
    10081 ccgacactgt tttccgggaa attgcgacca agggctttaa agccatggct gagacttcct
    10141 ttgctgtcaa aagggagctc aacggcccag tcgtgcggca ggtcaagcat ccgccatgcc
    10201 cggtcatcaa aacgggtacc cgcagcaccg tctccatagg atgcttttgc caggtaggag
    10261 aaataacttg tagcgtgatt aaagtctttg gctgcatccc agggatgccc gaatgcaaaa
    10321 cgccagccgg tatccatcag tatcctcgtc cttgggacca aggtctgttg ctgtgctatt
    10381 aatcctgtaa aacctgccag ccataagcag ccaatgagca accatccttt cataatagtt
    10441 tatgtttgag taacaggcaa cagcacaggc tataacctga tcactatttt ctttcctttg
    10501 tattcgatct tacggccagt ttccttcccg tcaaaggcga aaggcattgc ttgcaccgca
    10561 ctgaccactt taatatagat cgtccttgaa ttcagcatac cggcaaagct tccggtacga
    10621 tccccgatcg ttagcgtatt tgctgactgg caatatgtaa tggggatctc ggaatacttc
    10681 cctttttcgt aattataatt cactccttca tcttcatata aggtaaaaga agcatccttt
    10741 ccggtgtaaa tatttaaggt cagcggatca gcggctttct cacttgtata ttgcaaggca
    10801 ggacctgtgg ggatgaccga tcccgctttc acaaataccg gagttctttc atacgctgct
    10861 tctgatgtaa tggtttgtcc gcctttatag aacttaccag aataaagatc ataccaaccc
    10921 tgacctgccg gcaaatacac cgttcttgtt cttgctttat acgtatatac cggtgccacc
    10981 agcaatgaag ggccaaacat gaactgatcc ccgatattcc atgttttctt atctgcggca
    11041 aagtccatca ccaggccgcg caataaagta taatcatgat gataggcatg accagccagg
    11101 gaataattat acggcaggag gcgataccgc aattgattat aaaacaacat gctcttgtag
    11161 gcaggatggt cttccggggc tatattaaaa acctcgcgga ccggaaactg accatgtgaa
    11221 cggaacaatg gcagaaaggc accccactgg taccagcggg tttgcaactc acgccattct
    11281 tcttcatccg ctggcgccat aggtacttta ttgtaccgga cttcagtcgc gaatccaccg
    11341 atatccagag tccagtatgg caaacctgaa atagaataat tcaatccggc actgatctgc
    11401 gtccgcatat cttcccaacg ggcaccaata tccccactcc agatgctggc agcataccgt
    11461 tgagaaccgg caaaagcaga acgggtaagc aggaaaacac gcttcgtgct gtctgcaccc
    11521 cgctgacctt cataaatacc tttcgcgttc aacaacgggt aggcattcat aaattcagca
    11581 gccgtgccca tggccagcgg ctgcatctgt tgttttcgtt tctccgggga tacattggat
    11641 tgaatatccg gctcactggc atccatccac cacgcatcaa tctttttact gtagagcttt
    11701 ttattcaaca gctcccagaa cgctttacgg gccttctcat tgaacacatc ataaaaagta
    11761 gatacataac ccggacccac ccagtctttc tgacgatcag cgatattcct tgtgtacaac
    11821 cacccgtttt tattgaactc attataaact ggaatatttt cgtagacctt gggccatacg
    11881 gagatcatca gttgtgcatt atacttttta tgcagcactt cgatcatact gtctgcattg
    11941 ggaaaacggc tacggtcaaa atcctggctg ccccagtcat tctctttcca gtaaaaccag
    12001 tcttgcacga tattgtccat cggaatcttt cttttcctga attcagcgac cgtattcagg
    12061 atatcctcct gcgatttgta gcgttcacgg ctttgccaga agcccatagc ccaacggggt
    12121 acgattggag ctttacctgt cagttcccgg tagcctttca ccacctcatc cggcgaattc
    12181 ccctttacaa aataatagtc gatctgtgat ccggcagagg aaataaaact aaatgtatgt
    12241 tcatcttcca aaggggccgg aggaaggaat gatgccgaga cataggattc cccgccatcg
    12301 ggtatccatt caatttttac ttcacttcgt ttcgattttt caatgaaaat atcagcgagt
    12361 accgtccctg ggttccagga ctgacgccat ttgtcgaaaa caactttccc attaacgctg
    12421 agtttgagat agcctccata agtaaaacga aaggcatagt aaccggactg atctgctgac
    12481 aaactacctt cccagacaat tttacctgta gcaggcttaa attctttggg tgcccgctgc
    12541 agcaaatcat tcatatatgg ataccagatc tctgattctg ctttttcaaa caccagatca
    12601 ccgggtttat ccttgtcatt gtaatacgaa gtagtcagcc atccgggctg tcctttttta
    12661 tcatagagtg ctaaagcgga gagttgctgc attggacgca catcacccgc ttcactgtag
    12721 gagtagttgt cccagaggat gccgtaattt ttaacagaga ctaaataagg aaccgctact
    12781 tccgtattat tctgaaagaa aatcaccttt ttgcctttat aattccagct gtcatcctgg
    12841 tgttgtccaa gtccgtagta ggcttcctcc ttatccgatt caaacacctg ccggagacta
    12901 tacaattttt taccctcctc cattacgggc tgaaggcttt tcccatttat atcccgttca
    12961 cgcaggagcg tttttccgga ctggtcaaaa aatgaaacag cccccgaact cagctttatt
    13021 tttgccacca gttcttttgt cttcaacagt acatatccat tctgttcctc catctcccag
    13081 cccgtttttt ccggttgctt aattacggtc aggctttctt tgtcattaaa ggaaagttcc
    13141 ggagctgagg taacccgaat gattccgtct gcgaatactt tcaaacgtac cgcttttaca
    13201 ctcccctccc ggggttgata gggataaaca atcacccctt cccctgtttt gataatgacc
    13261 ggtttctgtg caaagacagt aaggctggaa ataaaaagaa gaaaggccgc aataaatatt
    13321 tttttcatga tcatttattg ttttgataca accataaaca ccgcagctct tccgggcact
    13381 accacccgta ttccattagt tgttgacccc gaataagggc tcagttcctt ataagtggag
    13441 gggccaccgg ccgcagatgc ggaagttatc ccattcacca gtgtttttct tgaaaactca
    13501 ccattatctg tgccgccggt cagtgtgtac cagtaaaaac ggctgccctt cttaaaattc
    13561 tttatatcca cctgcaccga cttgggatca gcggctttat ttataatggc cacccctgtc
    13621 tcccccgaag taaatgaaga accatagcta atcacatccg cattagttga attggcagcg
    13681 actaaccggt cgcccaggta ttgctgaaag aaatacatgt gatagaaagc cggccttgga
    13741 ttccactttg gcaccccatc cggctcatct ccctggttaa acataccatg atcatcgcca
    13801 ttactccagc cattggccag gtcccagcgg gcggtcatcc ctattttatt cttcagcgat
    13861 tccccgagaa ccatcacggc atgcagacca tttacatgag acacctgctg ttttgaaccg
    13921 gaagaagtaa tattccactc tccaaggatt accggcttct gtgtaagtcc tgcacccgat
    13981 atagtctgcc ttacataatc catcatcttt tgtgtttcct caaccggtgt attcaggatc
    14041 acttccgcag tggcattggt ctggaagggt gtgtaataat tatggacgat ataaaaatca
    14101 ggataattac cggccgctgc aaataatccg ctgttccagg tagtggctgt tggtgtccac
    14161 caggacaagg gggctgattc agacataacc gctccgatgt atatagtctt tcctatttcc
    14221 tgggcggctt tacgcatgga atcggcatat actttgaaat gttgtccata caattgtccg
    14281 gtcacaaatt ccggttgccc gtcctgatta gtagctgtat tgatacggta gcctgcttcc
    14341 cagttgccaa aattttcatt gccgatttcc cagtattgtg tccggccatt atcgtagcgc
    14401 acccagtcag ccgccaggtg tgcagcacgg gcaactgggt tggctccggt accataacgc
    14461 gcatatccat agttgatggt gatcatgcct ttgttaccgg tctgctgcaa catgctgtaa
    14521 taattgtcaa cagaaaaagt ccggttatcg gtattcttgc cataccagaa tccggctgcc
    14581 accgctgttc cgttatcctg tatcagttgt gcaggtgcat cggcaggcgg cgtatttggc
    14641 tgagcgttcc agaagaatac atcgctgata ctgcccccgg ggaaacggat caggtgcggg
    14701 tgcagattgg taatatttgt catcaggctg gtctccgtga ccatttgtcc catccataga
    14761 ttggcattag cggaagcata tgaacgtggc actttggtaa gcacattact gcggtcaatg
    14821 gtgacggtta ctcctgccgc agccggaaca gttgtattgg taaaggttgt aggtaccgtg
    14881 aatgtccgcg gctgccagtc atccataaaa aagccgatgg tattggccag gggcggatcg
    14941 acctgcgggt tcaccgtagt cgtatcacca acgggtggcg gtgttgtgcc gcctttcttc
    15001 ttgcaggaca atataaagaa cggcaggctg atcagaacac tcagatacag gaaacgggat
    15061 gtgattgtca tacaaaaaat ttattcgtta atacgtttca tcagctcaag catagccctt
    15121 gagttatggt aagggcattt ccagaacccg gccttatctt cattcataat agaatagtcc
    15181 tcatagatac cccaaaacca ttcccctttt tcatgatccc tgatatactg gcgtataaac
    15241 gcccaggtgt caaggcaatg ctggtaatac tttttctcac ccgtaagctg ccaggcgtta
    15301 taaaaaccta ccagggcttc tgcctgcggc caggaatgtt tctcccggat cagtttattt
    15361 tctgctggtt caaattcgta ccaaagcccc ccatccttat cctgcccctc agccgctgca
    15421 ttggtaatag gtatggcaag ttttctgaat aattcagtca aggctttatc ttcaatgatt
    15481 tctgcgcatt gttgtaataa ccaggctgct tctatatcat gaccaaatga tcgccgtgca
    15541 gattttgaat gccagtcatc gtcaaaaaac aaacggaggt gataatgaaa atgatcaaca
    15601 aagtactgtt caaatagtcc gagcagattc gtgatccgtt cttttaatga aggatccggc
    15661 cacacggagt aaagactggc ataggcttcg ataacatgca ggtgcgtatt catggtctta
    15721 cgctcattgt tatccttggc actcagacgc agatcaccga tggcagacca gtcacgggca
    15781 aaagcttcga tataaccgtt tttttcattg tcgaaaccat attgctctat gcatcgatac
    15841 aattcaatag ctgtttgcag cgccgcttca tggccggtca gttggtaata ggccgataag
    15901 ccatagatac agaaagcctg tccatacaat tgctttttgg tatcgagtgc ttcaccaccg
    15961 ggtttcaatg accaataaac acccccgtac tgccggtcaa taaaaaaatc attgatatag
    16021 gcatataccc ggtcggccat ttcctgatgt gccggatttc cggtgatggc tgtaccggca
    16081 gaaaatgccc aaaggatgcg gctgcacatc acggaaccaa ccgctgccgt tttgtctggt
    16141 atattgtcat tatccacctt accatagaac ccaccctgct ggttgtcggg tacatacttt
    16201 atccaataat taaggatggc ttccagttcg gatctcagtt cctggaggaa ataattccgg
    16261 gcaccggggt ttatatgttc catcgtgtat gtcatcctgc aaatgctgca ttagcagtcc
    16321 cttcttcttt ttcaacgggc aaagacaaat tacgatcgat cagttctgtc agggttttca
    16381 ctgattcagc tgaagtaaat tcatcagggg acgtattcat tacataatca agtaatttat
    16441 ctactgtgct ggtggccaca tgtatccgtg tatcagatga tgcgtaatag ataagtaccc
    16501 ggccatcttc ctccacaatc catccgttac tgaaaaccac attggacaca tcaccgatcc
    16561 tttcctctcc ttccggagcc ataaaataac cagcaggctt atagatcact ttggagatat
    16621 cgtgcagatc ggtcatgaac atatacagta catagcggag accagctgcc gtattgcgta
    16681 caccatgtgc caggtgcagc caaccctgtg ccgtttttat cggcgctggt ccaaggccgt
    16741 ttttcatttc gtacacagta tgatacactt tagggtcaat cacttgttct tcatcgatca
    16801 ccgctccttc catacagtcg caaacgccaa atccgatacc acctcctttt ccggcttcaa
    16861 taaatttatc ctgcggacgg gtataaaatg catagcggcc attgtaaaat tcaggatgca
    16921 ataccacatt ccgctgctgg ggagattggg ttttcagatc gggcaatcgt tcccagttct
    16981 tcatgtcttt ggtacgggca agtccgcatt gagcaattgc ggctgattga tctgtggcag
    17041 gtgcggaagg gtccctgcgt tcggtgcaga atacaccgta aatccatcca tcttcatgtg
    17101 cgatgagtcg catatcgtag atattggtat ccggctcatc ggtttcaggc atttgcagcg
    17161 ggtagtccca gaatttaaac tggtctatac cattcggact ttccgccacc gcaaaaaaag
    17221 atttacggtc ggctccttcc acacgggcta cgagcagata tttatcattc catttgatag
    17281 cgccaggatt taatacagca ttgattccaa aacgttgtaa caggtatgga ttagagtccg
    17341 gattcaggtc gtaccgccaa aaaaccgggg tgtgtgccgc tgtgagtacc gggtatttat
    17401 accggtcgac aatcccgttt ccattttccg ctgcttctct ttcattttgt ctggcgacca
    17461 gttcctcatg tgttttttcc agtctggcga gtcgactgta gaaattgtta cgcatattgc
    17521 tcgaattata gtgatcaatc ttccagctta tcccaccatg tttttttcag gatgagtata
    17581 atcagggtta aaattaaaat tgtaatcagt aatggcaggt gcatggataa caccacgtac
    17641 ataggtaata ccgtcataca taactgcgcg atgatgccca gcacgacatt aaacatatcc
    17701 agtttaaaac gacggttggg ttcaaaaccg ggttcctcct tcaccaccag ttcgtgtacc
    17761 ggtttccaga atccccaggg gcgaacggtc cggtaaaatt ttttaagtgt ttctgcctct
    17821 gttggaggtg cggtaaacgt tccgatcagg ctgccggcta ccgagagcag gaagagtacg
    17881 ggaaaataat agagaaattc aataccggta aataaccttg aaaagaccag ggcacccgtt
    17941 acaccagtaa gcataccaat gtaaaatccg ttggcattaa accgccacca gtaccatttc
    18001 aatacattag aggcaatata tccgccataa agtccggata cgatccactg aagaatgctg
    18061 tttacatcct tggcaaaaaa tccaaggatc acccccaggg ctactaccag tactcctacc
    18121 aggtaattca tgcggatgat ctttttgtta gaagcatcct tgttcacgta cttgaggtaa
    18181 atatcattca cgatataggc ctgggcggca ttgagggttc cggagaaggt gcccataaag
    18241 gcgcccagca atccggttag tacaagtccc aggataccaa ccggcagaaa actattgatt
    18301 gcaccgggca gtatgcgctc gaaatccaca cccccgttac cattactcag gttaagctga
    18361 ttataataaa gcaatcccag aactgtgagc ccgattacca tggcataccg taccggcagc
    18421 aggatgatgg atacaaagcc ggtcatcttg gacgcttctt taggtgattt ggtagaaagg
    18481 attttttgca tgtcataatt aggtgccggg ccggctgcag cagcaaatac gcctttaaag
    18541 agcatcatca taaacagcaa tccgaataac ccatatccat cctcctttat tttttcattg
    18601 gcttcattga caatacccga ccagtccata ttcaggttcc agccaaaaaa aggatcactc
    18661 catccggctg gtacattcaa tgtattaccc cgaagatgaa tcatggcgat tgcggcgatc
    18721 gcaatacaac caatggtcat gatcgcatat ttgatcaggt cacccagcac aatactgtgc
    18781 atcccaccca atatggagta gaacatggca aagagtgtaa agatgatccc gtaaaaatgg
    18841 gctacatatt gtggggcgat atcaaacggg atataatcct gaacaagttc ccagggaaca
    18901 aatatttcaa tgaatttacc cagaccgata aagccatagg cgagaaaacc aaggcaaccg
    18961 ataagggcaa aagccactac gatcgcgtga gatcctttta caccagcccc tttaagtccg
    19021 aaacgggtag ccagccattc tgccccggta ttggcattgg aacggcgtaa ccatttactc
    19081 aggtacatca tattaaatac ctggttgaat accggccata accaggggat ccagatactt
    19141 ttaatcccat aaacaaaaca aagggcgacc atccacatgg taccactgat atcaaacata
    19201 tcggatgcat cgctcattcc cagcatgtac cagggcagtt ttttaccccc catcaggtag
    19261 ctctctttat tcaccctcgc tttcttccgc aggaaccagc ccagcatcag catggtgatc
    19321 aggtaggaga caataatact gatatcgatc agggtgagct tcatccgttt taatttatat
    19381 cttttttctg gcttatttaa aacaggcagc cggctgagga atcagcccgc ctttcctgtt
    19441 ttacgattgc tttgttgctt gctttgctta tgaaatagtt attgatacaa ttgttcattg
    19501 aatgcttctt tttcaaacat tacgcgctcc aatgcggcaa acttccggaa gtccgcttcc
    19561 gacacctgcc ctttataggg ggcatagtaa tgcatttttt ttgtttccgc ctgatagccg
    19621 tgattccgcc agaccattac ataactaatt ttgttatcac cgatcgcatt cagtaatgtc
    19681 tgcgtccacc acgctgcgta aggaacggct tcatacccgg tttccgccag tgcaaatggt
    19741 tttccttttt cggcggccag ttcacccacc attttcaaac gccggttggt gttctcttca
    19801 aaccatttct ctttttccgg atcaccatac tgatacgtat cgaaactcag cagatccact
    19861 ttatcatcac cgggataacg ctccaggaat tcttcttttg tattaaaatc acctccagta
    19921 ttatataccc agagcagatt atgtaatttc ttttccgttt gcagataata ataagtaaac
    19981 cgccagaggg tctggtattc aaatacagta caggcattcc ggcaccacca gaaccagtta
    20041 ccgctgtgtt catgaaaggg tcggaataaa accggaatcg cttccccttt acttcctttc
    20101 aatgaaccaa taaaggccgc gactttatcg agccactctt tatacattgc atggttggcc
    20161 ccacccggat taatggaaga cacggaacca tgcgtggtat cccaggctcc tttgccccca
    20221 cccaacggac tgtctgcatg ccagctcaat gtaataacgc cgccccgttc atatccctgg
    20281 cggataaact gtttcatctt tttaaaaagc actccgtcaa tattcttatc attgcccggc
    20341 cgttcaatac cccccagttc ccatccgtat acagccggat aatcgcctgc ggcttctttt
    20401 acatcacttc ttccttcttt atatctccat tccaccccat aggccaggtc atcctggtgg
    20461 ccaaagagga atcctttatc ctgtagtgtc agcaggtttt tataaagacc aattgtttct
    20521 tttgttgcgg acggatcaac cgggccggtt tgtgcccaaa cagtataaac agccagcagg
    20581 caaaataccg ttatcaccaa aagccgtact gttttcattt gaattggttt aattgcttaa
    20641 tacaggaagt tataacattc cacgtactct tgtccgcatc gaaaacagaa tttaatccct
    20701 gttcttccac gggtggatcg cccagcacat catctccttc tacccagagt aactggcgat
    20761 gcgagggacg gcctttcccg gcgaaggccc agaaattagc gccacaaatc actccggcat
    20821 ttttctcagc atccaggagg taatcaaaga caaatgcata ataaagatcg cggtagatcg
    20881 tactgctttg cagtgaaaac gaatgttgat cccggggtaa tccaaactct tctatgacca
    20941 acggtttttt taatgcaacg gccacttctt tatgtcggtt gatataggca cccgtctttt
    21001 ccagcacctg atcaaaactc cggccaatgg cggtatcact gaaccaaccc cagttcttgg
    21061 gccagatatg aatcgtgaga taatcaatgt ttttgtcttc gtgtgtggaa cgaaaaacgt
    21121 ccatattctc cgaaccaata tcaccctccg caccggtagt cacgaggtga ttcgggtcaa
    21181 gggaccggat gaacgcagct gtttctcgaa taaacttttt atacgcatca atggctgccg
    21241 gccgcatggg gcgaggctca ttggcgattt cccaggacat gattgccgga tcatccctgt
    21301 attttttctt tgtaatgctg ttcgtgcgtt ccaccaatag ttttaactgg gttgtatact
    21361 ggctgacaca atcggcacaa tcatagaatc cgctggccag gtcccgcatt tcatcccaac
    21421 tgagttttcg tttgagcaca ctgtctgcta tttttccatt ccagttgagg tattgcagaa
    21481 atcccccact ccattcccag ttattactga aataaataat cgctttcatt tttttcttag
    21541 ccagttcggc cagtaaaaaa tcaagtcctt ccagtaattg tatattgaaa acaccaggag
    21601 ctgtttgcaa tgcgggctcc acccgtggca ccccattgat cggtccggcc ccttcggccc
    21661 cggccagcac gcgtacattc cggacacctt ttgccgccag aaaagcgact tcctctttaa
    21721 ttcgctgttt cccttttgtt gttcgtgctt ccagcccgcc gtaccaataa tttacaccga
    21781 tatacctgta cgttttttgc ccgagcataa accggccgtt ctgtacggta acaaaagggt
    21841 ctcctgccat tgcctgtaag aaggcggtca gggctacggt taatactaaa agcctcattt
    21901 gaaatgttgt gttcaacggt tgaaaaaaaa gccgtctctg atcacagtta cccgaaccag
    21961 aaacggctta aacgatgact gcttattctc aaaaagaatc cgggattgcc ggcccgggtt
    22021 caatgattta tttaaagaac ataatattat caaacaacag ggtctttccg gcttcctgaa
    22081 tctggaatgt aacattattc acatcgttgc tcagtcccgg ataagcggta gtcaggtcat
    22141 agcggaaata agtccattta ttagccggga tggtgactac tttctgattt ccccagttaa
    22201 tccggaattg cacgttttta tcaacatcgg ccccttttgc ccagaaagag aaataacgat
    22261 agcctgcaat attgatggaa ccgccattac ccagttgcat accaccccag ctaccggccg
    22321 gatcataggc tgctttcagg catttggcac cggtgatttt atcatctgtg gaagcattat
    22381 aatcgccgcc ccagccccag ttttcaaacc cattattgag ctgttcggtg aatacggtta
    22441 acgcctggcc cacgtttaca aattccatat cggtaatacg aataccggaa gcattggtga
    22501 gtttcagttt cgcccggttc gctgtactgg caggcatagt gacaacgatt tgtttccggg
    22561 tttgagaaac gatcgtgcag gcatccgtag ttccttctaa tacaacagcg gatacatcat
    22621 cgaggttatt gccggtgatg gtcacctgtg tccctgcttc gaaatcggtc ggaaacgcag
    22681 cggttacatt gggcagtgca atcacactga acggaacggt cagggtttta ccatctgcat
    22741 tggtgaagat gacattctgt ggtccgccaa atgcggtatc cggtacacgg aaaaccaggt
    22801 cggttgttgt attgagtgtt gacaggaagg gggcaggcac attattttta tcaaacacga
    22861 tagaacggac ctggccgatt ccgcttccct taagggtaat cacttcacct ccggctgcag
    22921 aaccggggtt cagttcacct gaactcattc caccggtact gacttccgga tttccatcac
    22981 tgtccttctg acaaccggtg ataaacaggg cacccagtcc cagcatcagg aggaagatgc
    23041 ttagttgatt tttaatgaat tgtttcatat aaagcttctt taaaattact gaatgaatga
    23101 cgattaataa taagcaacag gcggttgagc cagcgcagga tcctgtaata cttctccggc
    23161 aggaatcggc aggtgcattg aattctgcgt gaaggttaca tacatcggac tatcaaaagt
    23221 gccccggttt tgtgcttcga tgatctgctt ggctttagca aaaccctggc gctggatatc
    23281 aaaccagtaa tctccttcaa aagcaaattc gactctgcgt tctttcagga tatcatccgg
    23341 agtaagtgaa gttttctgca aaagacctgc tcttgcacgt actttattga aagcggctaa
    23401 tgcactggca tcagtagttg aggcattggt acccagcacg gcttctgcat aaataagcaa
    23461 tatatctgca taacgcagca tcatcgtatt aatacccgta ttcacaccga gtacggcttc
    23521 accaccgaaa gctgatccgg gtccaaccac atatttcgca atatttgccc gaacctgatt
    23581 cttctggcca ccatcaccaa ccggttgcag ggtatcatat ttataaccac ccgccataaa
    23641 tgcattatac gtcgcgttgg cattcttggg tttccattgc ggattagtcc agccatgttc
    23701 catcatcgag cctttccgac ggatatcgcc tgactcgtat gcattgatca tatcacgact
    23761 tggtgtatac agttcccaca tactggcttc ggaagtctgc aggttaccgg gaccacgatc
    23821 cggatttttc tgattacctg aaccccaggg atttccggtc agctgatgct gaatagagaa
    23881 cagggattca atattgttat tattggcact ggaattaaac atcgcgccat aatcattaaa
    23941 caggtcatac ttgccggagt tgattacttc taatgctttc attttagcac tgtcataatc
    24001 tttacgatag agatacagtt tcgccatcat tcctttggcg gaatactttg taacccggcc
    24061 cggaacatct gtttcaggca aaccggcttc tgcttttttc agttcttcga gcgcgaaacg
    24121 cagcacatcg gctttgaaat aacgggggat attgaaatca ccggaaagaa tggtagcacc
    24181 gggattgttt acgatcggcg catcacccca gatacgggcg atataaaaat aaacagtgcc
    24241 tttaaagaaa ttacattcgg caatagccgg atccaggaaa ctggcatcgc ccccttttga
    24301 ttttttaagt tccagtgcat tcatgtattc actggtccag ccgcctattt tataaaatac
    24361 tttccaggcg tccgcgatcc tgacggaggt agaggcgaag gaaaaattaa taaagggagg
    24421 atcgtcgcca ccaccggtat attcattacc ggccatcaca tccccgatgg catccatagc
    24481 acgattctcg taaccggccc agggtaatcc gtaaagcgta ctggtcagac cgcgggcttc
    24541 ttccgcggta ttataatagt tatccagtgt ggtgctgtcc agggccggct tgtccaggaa
    24601 ttccttttta caggagaaca gcgcaaccac cggcagcaga acaatagctg tatatcggag
    24661 tatatttttc atattaataa tgtttaaaca gtttgcttat tataattcaa ggttgatacc
    24721 cgcagtgaaa gaacgtggat tcgggtagtg accggcatcc acattggtca gccggatatt
    24781 attgttaaac acgcccactt ccggatcata cccgctataa ttggtaaacg tatgcaggtt
    24841 ttgtacggta atatacacac gggcggtgct gatctttgct ttggacagat acttcgccgg
    24901 tatacggtaa ccaatagcaa tattctggat acgcaggtaa gaaccatcct ccacataacg
    24961 gtcggacata tacacgttat ttacgtttgt attggtaaag cggggcagct taccatttgt
    25021 attcgcatcg gtataacgat ccatgacgga acgaagctgg ttataataag ggttgtccat
    25081 tttttccagc tgccagcgaa ggaagttgaa aatcttcgca ccatggcttc cctggataaa
    25141 tacagagaaa tcaaaattct tataattcac cgtgttggta aacccgtaag taaacttcgg
    25201 caaagggctg ccgataaacg taatatcctt ttcatcaatc actccatccg gcttgccatc
    25261 cgcaccacct acatctttat aacgtacatc gcccagccag gtatgggtct gatcaacaga
    25321 atatccaaac tggggcaaac tgttattcag gtcgtcctgt gtccggtaga gtccatcggt
    25381 tttaagaccg aagaaagaac cgactgcaaa accaggaatg gtacgggaga tcagatagtt
    25441 gtcataataa atacgtccat acaatgcggc aagatctccg gctacttttt ccagtttatt
    25501 tttaaactgg gagaaaataa tatttgtttt ccaggttaaa tccttgttcc ggatattagt
    25561 ggaggtcagg ctgaaatcaa taccagtgtt ggacatccgt cctgtattga tgacgggtcc
    25621 tttcaggtca tcccaccgat cacctactcc gatcaggcgg ggaccggtgc cgataagcag
    25681 catctggctg gttacttttt tatacacatc aatagcggtt tcaatacgcc cattgaggaa
    25741 ggagatatcc accccggcat tcttggtgat cacagattcc caggtcaggt caggattggc
    25801 caccccatag aggtagttgg aacttccaaa accaacgggg ccaggccaga aggaaaccac
    25861 tgaagtatag ggaggattcg gggcaccatt tggcatattc gtattaccta cttcaccgta
    25921 cccgattctt actttgagtg aattgatcgt cttcttcata ttttccgcaa acttctcatt
    25981 ggtcactgtc caggcaaaag aagcacctgg gaaattaccc catttgtttt ttccaaaaaa
    26041 cctggaagaa ccatctctgc gtatactcag gttcagggca tagcggttat cgtaggtgta
    26101 agtaccgcgg gcaaaataag attcaattgt accatcattc tttccaccac ccaattccca
    26161 ggtatcacga tcactgctgc cggcattgag gtcaagaata ttgttctgga gatccacctt
    26221 cttaccattc aggctttccc agtaattata ctgcgcttca tgaccggcag tggcactgat
    26281 ggcgtgttta ccgatattct tatagtaatt caggtaattc cgggcggcat aataatatcc
    26341 gttaccccgg tattcaaata actgactgcg taagctggta ttaccaaaat taccagtacg
    26401 ctgaaaggcc atattattat agagactcag tgagtaagcg aattcattcc gcagtgatag
    26461 ccctttcatg aattcaattt ccgcatagag ctgaccgaat acctgtgaac tggttgattt
    26521 attaccacgg tagaaactgc gggcaatcgg attgtcctgt gcgtattgga agcctccgat
    26581 tgtttgtccg ccaccacccc aggttccatc caggtttttc accgggatca gcgggctttg
    26641 cagggtaccc caccaaatgg ttccttctgc ggcatcagcc agcgttacat tctggatact
    26701 gcgggaagcg ttggcgctta cccctacttt gatccagttt ttcaactgat gatccaggct
    26761 gaagcgggtg gaataacgtt tgaaatctga gccaatcagg atccccttct gggtaaagta
    26821 gttggcagag agataatagt ttgttttgtc tttaccacca gagaaactca gctggtgatt
    26881 ttgtacggta ccggtacgga acatggcatc ctgccagtcg gtaccacgac caagaatact
    26941 tgggtcggcg aactccgcgc tgggtgtgaa gccaagcaaa ggagaaactt cattctggta
    27001 ttgtgcaaat tcacgcaggt tcatcaggtc cagttttttc tgaacgatct ggttacccgc
    27061 atacatttca taattgatct tgccttcacc ggatttacct tttttggtag taatcaggat
    27121 cacgccattc gcggcctgcg atccatagat cgcctgggca gaggcatctt tcaggatatc
    27181 gatggactca atatcattcg ggttgatagt agcaaggaaa ctgtttccgg tttgtccgtc
    27241 agacccgccc agtccggcat aacccgcact gaattttgta ttactggcaa agatcactcc
    27301 atccaccacg atcagggggt cattggcatt gatggtagtc accccacgaa tgcggagtga
    27361 tacgccacca ccgggctgtc cactgttatt cactacggtt acacccgcaa cctttccttg
    27421 cagggcctgg tcgatacccg ctaccggaag atccttcagg tcctttgttt ttactgaaga
    27481 aatcgctgaa gatacatcgg aacgttttgc agtaccataa ccaatcacca ccacttcact
    27541 cagtttggtg tcggttacat ccaggctgac caggatattg gtcttgcctt ccaatgaaac
    27601 ttctttgggt tttgctccca cataagaaac caccagggcg gtggcattcg ggggaacggt
    27661 aattttaaac gcaccgtaat tgtcggtgat caccgtggtc ttacttcctt tcacggagac
    27721 agtggcattc accaggggaa ccccgctctg atcggagacc acacctgaaa taactctgtt
    27781 ttgtgaaaat acggtaaggg aataaagtac aagcacaatg atcattgtga ttgcacgggg
    27841 gaatcgtgtc tttctcatac agcaagaaaa tttagactca aaagtaggca tatatactta
    27901 tgccagaata atactatttt atctgattat tgtatttatt aaccctgatt taagcccaat
    27961 attccggatt tttccttttt agcaggaaga aaataaaaga taataaaaaa taaacagatg
    28021 tcaatatgct acttttttat ctattagcta gtaactttat ctcaaaggcc tgccatgcat
    28081 aagaatattc tccgggaaat taccccgctg actcccagtg attgttttac catcttcacc
    28141 cgggtaaaga cggactttga ttttcccttg cattaccatg aagaactgga actgaacttt
    28201 atacacaatg ccaaaggagc ccggcgggtg gtgggcgatc atattgctga aatagatgac
    28261 ctggagttgg tgctggtggg atcaaatctg ccgcatgtat ggcagaccca taaatgcaaa
    28321 ggacggaaga tcactgaagt cacgattcag tttcacaaag atctctttga tgaaaaattt
    28381 ctccgccgca accagatgag ttttatccgc aacctgctcg agcgttcggc caagggcatt
    28441 ctgttctccc gtaccacagc agaacaactg aaatcacggc tgctggctct cgaacaaaaa
    28501 cagggctttg actccgttct agaactggta tcgattctgc atgatctctc tgtatcccga
    28561 aatatgcaca cgttatctga tgcgactttc agcaatgcgg aactctctta taacagccgc
    28621 cggatagaaa gagcctttga atacatgaat gaaaattttc acaagaacat tacactgact
    28681 gaagtctcca aactggtgaa tatgacggat gtctccttca gtcgtttctt taaagccaaa
    28741 acaggaatta cgtttattga cagtttactg gaattacgtt taggccatgc ctcccgctta
    28801 ctgattgata ccactgaatc ggtttcagag atcgcttacg actgcggctt taataatatc
    28861 tccaacttta accgcctctt taagaagaag aagaactgca ccccaaaaga attcagggag
    28921 agctacagca cagacaaccg ggtgtttatc tgatggccat ccggcctatg ctcacttcct
    28981 gatccactcc tgtaaaaggg ttagatatca ataattttac atttttttac atacttattc
    29041 ggaaattttc cgaataaaat gatatatttt gtcatataat tgttttattt ttggtactaa
    29101 atcggaaata ttccgaatta aagccaatat tatgtacttc caggaagcca tcgccccata
    29161 tgccggtcag ccgctgaccc agcaattgct actggatatc ctgagggagt acaaaaggcc
    29221 ttatgataaa attactgagc tggtaaaaca ggaacagctg atccaggtaa aaaggggtct
    29281 gtatattccc ggcgctaaac tgaaactgcc gccaccggta tcctttctgc tggccaatca
    29341 tttatacgga cccagttatg tttccctgga ttcagccctg tccttccatg gattgattcc
    29401 ggaacgggtt tttgaaatca gttcaatgac actcagagcg gcaaagacct atgatacaaa
    29461 tgcgggccgg ttcagttaca ccaaaatgcc ccagccctat tatagttatg gcattcagtc
    29521 tgccgaactg gccactaatc aggtggccct ggttgctact ccggagaaag cgctctgtga
    29581 tcgtatcatc aacacttccc agcttcagct gcgtagcgtt aagcagacca ttgacttcct
    29641 gacggaagat ctccggatag aaaaaagcag tctgcggcaa ctggatgccg gagctatcag
    29701 ccgttggatc aaaatagctc caaaaaaaac aagtttagcg atgttggtca aaacactgaa
    29761 tagtttatga taaaggaatg gctggaaacc tataagcccg cgcatacgga ggacgctttg
    29821 caggccctgc gggagatcat gcaggaaata gccctggccg gattacaacg cagtggcttt
    29881 tttgaagtgg ccgcatttta cggaggcacg gccctccgta tttttcatgg gctgccccgc
    29941 ttttcagaag atctggactt ttcattactg gaaaaaaaca acgacttttc cctcgcccct
    30001 tacctggatg gaatggaaaa tgaatttgcg gccctcggta taaaagtcag tgtgcgggaa
    30061 aaaagtaaaa caactattac agcctttgat tccgcttttc tgaaacccga taccacctgg
    30121 aaggaactta tcataaaagc agtactcccg gatgaacgga tcaaactgac cccggagata
    30181 aaaatcaaac tggaagtgga tacgatgccc cctcccggtt ttgatacaga agaaaaactc
    30241 gctttaaagc cattctcttt ttacaccaaa tgttttaccc tgccggatct gtttgccggt
    30301 aagatgcatg ccttactatt ccgcaaatgg aaaggacgtg taaagggaag agattggttt
    30361 gacctggaat ggtacattca gaaaggagtt cctttgcacc tggatcattt ccgtcaacgg
    30421 gccattgaca gtggtgactg gacaaaaaaa acgatgactg aaaaacaatt taaccagctg
    30481 ctgaccgata aaataaaagc catatcgttt aaagcggtaa aggaagacat tattcgcttt
    30541 ataccggatg cgaaagtgct tgagatctgg tcagcggagt attttaatga tttatctgaa
    30601 aaaattaaat tcgggtaacc gtatttttat ctgagcagcc ctgagactaa aacagtatca
    30661 gcaaaatctt tcaggctggc atagatttct tttctttcct cggcaggtat aaataattta
    30721 tactggatgg tgtccatacg cggaatcctg acctgccatt taatactgtc tgttaccaca
    30781 ttagtcttcg ttgtacggat gaatacccgc acattcaggt gattgtatgg atgttgcata
    30841 aaccgttttg tccattcctc cttttccgct tcattcatca atctgttgtt ttctttgagg
    30901 aaatccgtct gctcctttaa gtatttctga aaatgttcac ttagtaatgc ttttcgctcc
    30961 gtagtatcca gcactaccca ctttttcatt gtaaaacaga ttcgtaaaaa atcctgcagc
    31021 gaatcagccg gaaccggtgg ctgataaaaa atgataacag gccggttatt agctgttctg
    31081 ccatatggct tcactataaa cgccattaaa cagaaaacaa cacttgtaaa aaaaaccact
    31141 ctaatcatgt cactagttac tgaaaatttc aaatacgcac aatactactt tcgggttata
    31201 aaaaaagaac ccgcttctca gcgggttctg ctttaatggg taccatcttt atttggtttt
    31261 ccgtcctaga ctaatttgtt ggggagatca accacaacct ccctggttgc cgcagttcag
    31321 gcatttgtag caggcgccgc tacgcatcat gatattaccg cacacgttac aggcgggtgc
    31381 atcactctgc atatttttcg ctaccgcgtt catggcatcc atgctgcttt cactcttcac
    31441 tggttgtgcg gcccgttgct gggctttcgc cggctggggg gctactgaac cggaagaagg
    31501 tgttacacgt acactggaaa gctcaggctc ttttacatat tccagggggt tcgaagggac
    31561 ttcatcccaa tcgtcaccac cggtattcat cacttcgggt ttatccagca catgtacgag
    31621 gtcggtacgt ccgaggtact cataacccaa tacacggaag ataaagtcaa caatagaagt
    31681 ggttgttttg atattcgggt gttctacaaa tccggatgga tcgaaacgtg tgaaggcgaa
    31741 tttatccacg aactcttcca ggggcacacc atactgaaga ccaatagaaa cagagatcgc
    31801 gaagcagttc atcatgctgc gcatggtagc accctccttc gccatgtcca cgaagatttc
    31861 tcccagtgta ccatcggcat attctcctgt gcggaggaat acggcctgtc cgctgatttt
    31921 ggctttctgt gtgaaaccac gacgtttggc cggaagcgta cgacgctcca cgatgctggc
    31981 gagtttacgt ttcagcttgg tatcaggaga agcctgtaca cgtttctgta cttcttccag
    32041 cagttcttcc acggtcagtt taccgaggtc aacaatgttg gattcagcaa caggcgcctg
    32101 ttcaaccacg gctgcctttg tttcttccac tgcagtgtct tttttctttt tgtccgactt
    32161 gttactgagc ggctgagaca gtttggaacc gtcgcggtag agtgcgttag ctttcagtcc
    32221 gagcttccag ctcaggtgat aagcatccgc aatctcttcc actttggctt catgtggcag
    32281 gttaatggtt ttggagatgg caccactgat aaaaggctgg gtggcaccca tcatgcggat
    32341 atgtccgtgt gcgtgaatat aacgctcacc tttttgtccg catttattgg cacagtcaaa
    32401 aacgggcaga tgttcatctt tcagtccggg cgcaccttcg agcatcatcg taccgcatac
    32461 atagtcgttg gcggcttcga tctgttcgtc ggtatagccg agggcttcca gcagactcca
    32521 ttcaaaatta ttatactgtt cgggagtaaa gcccaggcgt tgcaggcact cttcacccag
    32581 tgtatactta ttgaaaacga atccgatttc aaaagcagtg gcggcagcag catcgagacg
    32641 cttgatctcg tcggcgatga aacctttttc gctcaggctc tgatggttga caaatggcgc
    32701 accggcaaag ctggcggcac ccactgcata cttaatgata ttgtctgttt ctttttcaga
    32761 gtaacctaaa tttcgcaggg cagttggtac agactgattg atgattttga aataaccacc
    32821 accggacagt tttttaaatt tcaccagggc gaaatcaggc tccacaccgg tggtatcgca
    32881 atccatcacc agaccaatgg taccggtagg tgcgattact gttgcctgtg cattgcggta
    32941 accgtatttc tctcccattt ccacggcatc atcccaggca gaacaagcgg ctttcagcag
    33001 gtaatccggg cagtatttta ctttgatacc ttgtggcttc aggctcagtt gttcatactc
    33061 atctgcatca taggcagcca ggcggtgatt acgcatcacc ttcatcatat gtgccttatt
    33121 ttcttcatag cgggggaagg cacccagtgc gcgggccatt tccgcagaag ttttataaga
    33181 agttccggtc atgatcgcgg tgatggctcc ggcaatcgcg cgggcttctt cactatcata
    33241 tggaattccg cttaccatca gcatggttcc gagattcgca taaccgagtc cgagcgtacg
    33301 gtattcgtag cttaactgcg ccacttcctt tgaagggaac tgggccatca gcacagacac
    33361 ttccagtacc acggtccaga gacggcagtt gtattcgtac ccttccacat caaactgatt
    33421 gttgtctgcg tcgtagaact tcatcaggtt ggcagaagcc aggttacagg ctgtgttatc
    33481 gaggaacatg tactcagagc aggggttgga agccctgatc tctccacctt ccggacaggt
    33541 atgccactcg ttgatggtcg tattatattg agtaccagga tctgcacaac gccaggcggc
    33601 ataggcaatc tggttccaaa gttcacgtga aggcactttt ttcatcacgc ggccatccat
    33661 acggccggtg agttcccaat cctctccttt ctccagtttt tcgaagaatg aattcgggat
    33721 acggatggag ttattggagt tttgtccact tactgttttg taggcttcat cttcgtaacc
    33781 ggaaggataa ccggcattga tcagtgcacc tactttcttc tcttcttcca ctttccagtt
    33841 gatgaactcc acgatctccg ggtgatccag atccaggcat accattttag cagcacggcg
    33901 ggtcgtacca ccggacttta tggcacccgc agcacggtca ccgattttaa gaaaactcat
    33961 cagtcctgag gaactaccac caccgctcag cttctcgcct tcgccacgga gattggaata
    34021 gttggttcct acgccggaac cgtatttgaa gatacgggcc tcacgcaccc acaggtccat
    34081 gatgccgcct tcattcacca ggtcatcatc aacactcagg ataaagcagg cgtgcggctg
    34141 aggacgctcg taagcgttct gtgacttctt cagtacacca tccactgcgt ctacataata
    34201 gtgaccttgc ggtttaccag caataccata gctttcata
//
LOCUS       DAOPMW010000004        32988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1125_length_32988_cov_8.474236, whole genome shotgun sequence.
ACCESSION   DAOPMW010000004 DAOPMW010000000
VERSION     DAOPMW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 32988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32988
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_32988_cov_8.474236"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..488)
                     /locus_tag="PLU11_00400"
     CDS             complement(12..488)
                     /locus_tag="PLU11_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN57590.1"
                     /translation="MNSPVTFNCVIADDDEIDRLTTVSFAKQYPFLHIAGVFSTADEV
                     LELAKKSKPEVLLLDIDMPGMSGLELRQQLIDIPACIFVTSYPDYAVESFGLSALDFL
                     VKPLKADRFELTMRRLQDYLLIRQKAELLDFTLGSDSLFIKDGHDHIKIQLHEVVF"
     gene            784..1617
                     /locus_tag="PLU11_00405"
     CDS             784..1617
                     /locus_tag="PLU11_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57591.1"
                     /translation="MKRILLFVLLSSMLLFTGCKKNSSPVSAVIAKCKPVSEYTNLSG
                     NAATYEYVYGTDGNISSLKKYIGGGYHVLADSLLVFYDHTERFTPGITMGSFNKVNTV
                     FNANIFTGSPTRADISITLDGVEQRNYYTYSFSYDTKNRLAKISEQTNTVLNDLEYDL
                     FIIYNDQNNVSELRYEATTGPRTVTVIAASGYDNKHTPFAGIINWPMLMHAGWASSDS
                     EPLFTALSKNNPLGFEIPGWKRTITYAYNDNGFPVTRFSTNTTASATYSFEENFSCQC
                     N"
     gene            1647..2156
                     /locus_tag="PLU11_00410"
     CDS             1647..2156
                     /locus_tag="PLU11_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57592.1"
                     /translation="MKKILSTCILGISLLTSCGNNEQTQAMEDGKKMVSALKEMQPGG
                     IATSVDGWTMTAKFNGKPWTANSLMSPEVAGRIVGENNGESISLPYDRRGMVVGKKTR
                     FSQNNAVDLMTNDEVALWGGYAGEMEITKVDDEWAEGKFFVTGSSDSPAKQMKVTDGF
                     FRISLIKKQ"
     gene            2178..4145
                     /locus_tag="PLU11_00415"
     CDS             2178..4145
                     /locus_tag="PLU11_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57593.1"
                     /translation="MKTCIVILSLFLFCFDSNAQVLKKLGEKIKRDSEWRIRSKADQQ
                     VGRGIDSIVAIPKKVKDKKKAKKEEARKTETQSAAGKTENGSSGPLQTDNRSTQSKLS
                     EPGNDENDMEPRDGYAMLTLSANTVFTGGSITISGESIKYKNYKEVEITVTGPSVKDA
                     KSIPLNADGKFATVWFASGKAGEYKVTLKGSDKKATVSEAFTVYEIDGLDNLCDENIN
                     LITKAFDHLKETAEQVKNSLGSKDKAELDKKLEEVKEKVDLLLKLLKDLNVAGKDISK
                     LAKSGKNLSPNLADNLSVLNNKLKEQREKMEQVEEYRNHKPADNTVCEYLVLMNEACA
                     AFSVYSNIECFAFKGIIKNIILDKGVPLATGTINARAKGLSSPNDFLLKEPAKIFSTA
                     ALDAESLSSKLGTAGFCGDIVQFATDMLMNKYCGVFKGKLKHDYTIEFRNKSGQNWWT
                     YAVEMKAVLALRYPKDKGGGNIIRMKGNLEGNATKFSFFEDLEKNDEFQEGSKGKIEV
                     VPIKAYIPFSVSAATSEIDVMGFGAIARGLATPAYFNIPVDAEYDVNENKIKLFLNAP
                     IIDFSPAIRNQLVFLLIGGDLLPYVKRMSFPINKVFRTLGSVVRDNHEFPVVKDAKGI
                     LSFDGKANKHIGDRSSVIETDLNFTISAKKD"
     gene            complement(4146..4823)
                     /locus_tag="PLU11_00420"
     CDS             complement(4146..4823)
                     /locus_tag="PLU11_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN57594.1"
                     /translation="MSLYTYSPATDFSPVKKADGLMIAESTDALLLSIISHTRIEEVT
                     KRLANDNKAFVAWINNIPAAFGWMARDQAVIGELNHGFILPEGNRYLWNFRTLEAYRG
                     RGIYPALLQYILKQGDPDAERFWIIHAPENQASLKGIRKAGFEFVGKLYTGADGLATI
                     EAGEAASSFREELEYMDITMSADTATSCWNCSSPYLKKRSPECCCQEAGVKCVGNSGA
                     VVYMSNE"
     gene            complement(4869..6194)
                     /locus_tag="PLU11_00425"
     CDS             complement(4869..6194)
                     /locus_tag="PLU11_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HPN57595.1"
                     /translation="MKQESKTVEYNLTASEFNWEIKPGKTISAWGFNNQVPGPILRAR
                     KGDTLVINLVNNLPEPTMIHWHGIRLPAAMDGTGEVQRPVMPGESFEYRFTVPDAGTF
                     WYHSHQNETIQMERGMYGGIIIEEESDLLVDDEKLLLLDDMKLTGDNEFKQLGPVGRW
                     IERHDGREGSTNLVNGKEEAILYMHAGQTERWRIINAASARYFTLSLDGKPFKVIATD
                     GNLLEYARIETEWLITPGERFDIVAGPFNEGDQFSIRSLPYNRVTMLTAKNQPYARVQ
                     VLERKESVAHLPEKLSDIIALAPQDAPVTRKIKLSVDPSLKHIIDFTVNHEMHGMDKP
                     VMVGELQVWEVSNTSLMDHPFHLHGFFFQVLEDNGKAPAYKAWKDTYNLKPKTTIKIA
                     WMPDNRPGIWMYHCHIIEHHAAGMMANFEVIDPADPSPSGKGSFAGCHS"
     gene            6447..7274
                     /locus_tag="PLU11_00430"
     CDS             6447..7274
                     /locus_tag="PLU11_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPN57596.1"
                     /translation="MMKTKTRLLLNSIAALSTAMLLFTVSSCKKEVFSQGTVKSFSLQ
                     SAANGVTYDIKVALPSGYNPAAGKYATIYLLDGEEIFDFTANSCQEIAGKNGVANALV
                     VSIGYGQDRSIDYTPTKISNSTGGAPQFLDFIASQLIPKMEQDYNVDTSRAGRVILGH
                     SYGGLFGAYTFCVNQKLFGHYLLLSPSLWFDNLVTLQLEKENRALNRISPALVFMGIG
                     EAENSGRMQAPFESFYQSIRDQYPAVTLSKNREKNLAHLGSRNPNIIKSLNFYFQNR"
     gene            7317..7931
                     /locus_tag="PLU11_00435"
     CDS             7317..7931
                     /locus_tag="PLU11_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57597.1"
                     /translation="MNKRKFSIVFLLVLVYATNFAQLKIRVVTDKHHTVRSFTIDPAI
                     GFHTNFGSDFLLSTLVQWNPLKYLSLASHSSFAFNNIFQRDFNGVKTNYNYSILQKFG
                     AGTTFYSRKSSNTFLLMAGMKYTSFKETMKNPDNQPIKAAINSFSPDYGLMYALRKGV
                     KNRFFTFRMYVPLYPWPMKGSDISYADGNLNNIALELGVGLKIK"
     gene            8046..8621
                     /locus_tag="PLU11_00440"
     CDS             8046..8621
                     /locus_tag="PLU11_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN57598.1"
                     /translation="MASTNLLVKNMVCHRCILSVEGILRQESIPFRKVIFGEIELVAD
                     LTPEQKDRLEAQLEQVGFELIDNRSSALIEKLKQLIIKRARNEAGETDNKLKLSAYLS
                     RKVNHEYTYLSSLFSSVEGRTIENYFIEQRIEKAKELLIYGQLTLSQIAFDLEYSSTA
                     HLSTQFKKITGLTPTYFKEIGLAKRKSIDSI"
     gene            9014..9928
                     /locus_tag="PLU11_00445"
     CDS             9014..9928
                     /locus_tag="PLU11_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPN57599.1"
                     /translation="MQKVAFIIPPTVELLDLSGPAQVFTEAKVLGMEIEMEFYQYSEE
                     PVSTAGLGFGKLENFKTARLKEGDFVIVPGMDNDYVNSFSFKAEREFFNWLKECADKQ
                     VTICSVCSGAFALGHAGLLKDTECTTHWRRVQALQTQFPQARVMADILYIKSNNVYTS
                     AGISAGIDLALAILEDLKGPLFTHKVARGLVVYHRRSGTHKQQSIYLDYRNHINPQVH
                     EVQDYLIDNLSRENDIETLASLVGMSSRNLSRVFKEKTGSTILEYLTLLRKEFASTML
                     NNPDYTIEYIASKCGFKTARQLQRILKK"
     gene            complement(9945..11081)
                     /locus_tag="PLU11_00450"
     CDS             complement(9945..11081)
                     /locus_tag="PLU11_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN57600.1"
                     /translation="MKIVKRKIQKQIEDKIGKNKVLLIMGTRRVGKTVLIHEIKAKFK
                     GESLLLNAEDFDIRDILKNRTAANYRKLIGHASLFILDEAQVIEEIGAILKLMIDSMP
                     GLTIIATGSSSFDLLNKAGDPLTGRQYQFHLFPLAQLEIGEKETYTETVQQLDERLIF
                     GSYPEIFQLSSYQEKSVYLQQLVQSYLLKDILAYEGIRNSDKIIRLLRLIAFQLGREV
                     SYTELGTQLGMSKNTVESYLDLLSRVFVIHRLPAYSTNPRKEISKGSKWYFYDNGIRN
                     AIINDFRLPALRNDIGSLWENYLLAERIKRNTYQGNNVQYYFWRNYNQQEVDLIETTN
                     GKISAYEFKLSGDKKIKAPPAFTGSYPEASFTIISKDNYLDWIL"
     gene            11313..12923
                     /locus_tag="PLU11_00455"
     CDS             11313..12923
                     /locus_tag="PLU11_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HPN57601.1"
                     /translation="MENLTIIVFLLFGVAFIGILSNRFRFPFPIALVLCGIAISLIPG
                     LPVVELDPEIVFLLFLPPLLYEAAWNTNWHDFKANIRPISLAAIGLVLFTTVLVAVVA
                     YYLVPGFGWAESFLLGAIVSPPDAVSATSVTKGLGLNPKIITLLEGESLVNDASGLVA
                     YKYALAAVATGNFIFWKAGLNFMLVAAAGISIGLAIGYLMYLIHKKFISDPVVEVTLT
                     LLTPFTSYLLAEHFHFSGVLAVVTTGLYLSFRSDVILTHQSRIMGYSVWQVVSFILNG
                     LIFVLIGLQLRNIMKGIGNEYQTGELILYGAIVSLSVILIRFIWVIPAAMLQRMLNRS
                     LRKKEIYDTKNLVIFGWAGMRGVVSMAAALALPLTFPNGQAFANRYLIIFLTFCVILS
                     TLVLLGLTLPLLIKKLKTTPYSIAEEEYSIRTQVVSESITHIEENLSLVSDDLLNNIK
                     SKYEIKYNRLQHTDLPPDYFGDKPRVPENIFNAFTRLQLDMLEIERKAVNQLHRSGAA
                     NQEVLRKIEYELDLEETRLKMEFGSLGG"
     gene            12976..14373
                     /locus_tag="PLU11_00460"
     CDS             12976..14373
                     /locus_tag="PLU11_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN57602.1"
                     /translation="MSLRQFISSRKILRSFILLKENVTLKWKEVAELFGLHDDVFENP
                     RHLPLSKPVSVILIGAGHRGNIYAGYAHKNPREMKIAGVADSNRNRLLQASKKFNLPE
                     DRCFSDWEDVFKEPRFADAVIIATPDNLHTAPCLKALEMGYDILLEKPIAPTEEECRL
                     ILAKARSHNRIVGVCHVLRYSPYFRELKEVLDAGLIGNIISVQHMEPIEQIHMSHSYV
                     RGVWKNSKTATPIVLAKSSHDTDIIRWLVNSPAKDVHCFGNLKWFTNVNAPEGSTERC
                     TDGCKVEKSCAYSALQIYLRDRKRLYVFDMPSNKSLWDDFILTQLRTTDYGRCVYRMD
                     NDQPDHLTVNMLFENGVTAAFSMEAHVSYEGRRTRIMGSKGDIVGDMENFVLTDFKTR
                     KVTSWEMKTDAHGGGDHRLVKDWLQAVAQQDKSILSSSIEVSIESHLMAFAAEKSRLN
                     RTIEEINLDLDKATY"
     gene            14426..15664
                     /locus_tag="PLU11_00465"
     CDS             14426..15664
                     /locus_tag="PLU11_00465"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPN57603.1"
                     /translation="MLELTADELKIDSYSCKREQVRDFYIIAAGKAAGAMAAEAEKVF
                     GAFITGGLCITKYDHALPLQRLKMLEAAHPVPDENSVEAGRQVLQLLKGRSENDIVLF
                     LISGGASSLVTDLPSGTSITDLTKSYQLLVNSGAAIDEINCVRKHLSRIKGGQLAKAA
                     YPARIISLLISDVPGDDLQTIGSGPTVPDHSTYEEALSVVTKYGLQESLPASIWQHLN
                     MGVQGALEETPKPGDPVFRSVYTRIIANNYKAAAAAKMEAEKSGFHVQWDKRLMTGNT
                     DAEAQEFSQQLLHYEGPLPALFIRGGETTLRVSGNGKGGRNQHFALRALLEMKTHWNK
                     PSGFAILSAGTDGTDGPTDAAGAIIDSVMLTNPDLSVTTIQTHLTHFNAYPFFVQTGG
                     LLITGPTQTNVMDMVIGMIF"
     gene            16282..18846
                     /locus_tag="PLU11_00470"
     CDS             16282..18846
                     /locus_tag="PLU11_00470"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN57604.1"
                     /translation="MLTLVTIATLLIGVFLMASCRKSTDMPFTGDEPVSNTDSVSVMR
                     EAAALAVNTYYLSPSGNDNNSGSIDQPWFTLQKAWTVLAAGDLLYLRGGKYTYTVQPY
                     LTGKSGTAGNLIKVWAYPGESPVITRGTSFNKSNGWHRGMVYLSGNYIHLKGIRFTGM
                     YTDDNQVDAGLQTYNVNNCVFELLECDNNVEGMIVENNSNNNLILNSDFHDNFSNYGG
                     SNGGNSDGLGLTYMIGQNTTTTVRGCRAWNNGDDGFDTFENSGYVLIDSCWSWHNGYN
                     KGTNTSAGDGVGFKLGSDFLTTPAGVGVVKRRLQRSLGFDNRNAGAHINEADFSTEIF
                     NNVFFRNKITGLNFHYNNRVHTFRNNVSFGNTDKDVEISGNSIRSNNSAGTAGNDGGW
                     TTNASAADFLNTDTSGIRNARQANGSLPNINFLKLAAGSDLIDAGMNVGLYFTGNNPD
                     RAALEYGGVSVPVVNLPPVVNAGVDKTITLPVNNVTLSGSATDADGSIAVYAWTRTSG
                     PSTPTLSGANTSSLSASNLVAGTYLFRLTATDNNGATAWDETVVIVAGSGTPANLAPT
                     ANAGTDKTIVLPVNSIALTGSGTDPDGTIASYAWTYRSGPSTPVLTGATTANVTAGSL
                     VAGTYVLRLTVKDNGGLSGFDDVNIIVSASTTTNLPPVANAGPDKTITLPTNTVTFLG
                     SATDPDGTIASYNWTYRTGPATPSLTGARTATLKASNLKAGTYTFRLTVKDNKGKAAF
                     DEVNVIVKAAASTGGTKPISVSNAVADAGYSYYVVQDFGILPDDGANPVRSTLRIFEN
                     GVELNPAHSTHYDIQTIGRGRFSHWGDGSFIVLYFSASDNTNPKTNGRTYTYTTVQ"
     gene            18975..19412
                     /locus_tag="PLU11_00475"
     CDS             18975..19412
                     /locus_tag="PLU11_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57605.1"
                     /translation="MPAPSTKASTFLLFPWGLLSIVLVLGLNLVLDNDWFSKNWSWIA
                     LVLIIPAAILENFKVGYSTMRIMFMKVVYSLISFLLVGMAFGSALNGWHIARFQKIAD
                     VKTILLPWYVVLALSILLLIQVAQLFSMLKAVKREFMQMDPDQ"
     assembly_gap    19530..19629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19844..20953)
                     /locus_tag="PLU11_00480"
     CDS             complement(19844..20953)
                     /locus_tag="PLU11_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HPN57606.1"
                     /translation="MGAGCASLSLLLRMIRSGRFSDKKILLIDREPKTKNDRTWCFWE
                     AGDSFFESIVYRKWPRVDFLSDTYQATLTISPYEYKMIRGADFYQYCFQEISRHPQIE
                     IIYAEIGGCSTDEKSTTLVLNQAQFQLPPAVVFNAVYRPGTSRLNVLQHFKGWVIETE
                     QPVFDPGKASLMDFRVHQQEGTTFAYVLPFDAHKALVEYTLFTPQLLASEQYDQELKN
                     YIRDFLKITQYTVKEEEFGIIPMTDEKFPIQQNGVIHIGTAGGQTKASSGYTFQFIQK
                     QSDRILELLLKGESLSHLKPTPWRFRFYDNTLLYILYHKTLPGRKIFSTLFKKNKATQ
                     VLKFLDNESTLREELGIISSLPTWPFLKAALMQLR"
     gene            complement(20996..21511)
                     /locus_tag="PLU11_00485"
     CDS             complement(20996..21511)
                     /locus_tag="PLU11_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene hydroxylase"
                     /protein_id="HPN57607.1"
                     /translation="MDILLYILVFFGTFFVMEGITWCTHKYVMHGFLWILHKDHHQVR
                     PGVFEKNDLFAFIFAVPSFLLIYFGTYDHVWWMQSIGFGIMLYGAAYFLVHDVIIHQR
                     FKWFSRSNNTYVRAIRWAHKMHHKHLNKEQGESFGFLYVARKYWQKVQRDKQLMAGAR
                     RVEYAPEAEEA"
     gene            complement(21524..22069)
                     /gene="idi"
                     /locus_tag="PLU11_00490"
     CDS             complement(21524..22069)
                     /gene="idi"
                     /locus_tag="PLU11_00490"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPN57608.1"
                     /translation="MDLTQVILVDEQDQILGSCEKLEAHRKGFLHRAFSVFVFNRKGE
                     MLLQQRARNKYHSGGLWTNACCSHPAMGENTDDAVHRRLVEEMGFDTPAKKLFDFVYK
                     AEFDNGLTEYEFDHVYSGEYEGEIRCNPDEVMDYCFKPMPAIAESLQTRPGLYTAWFQ
                     LAFPKIESWWQQQYSTPALTD"
     gene            complement(22071..22907)
                     /locus_tag="PLU11_00495"
     CDS             complement(22071..22907)
                     /locus_tag="PLU11_00495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPN57609.1"
                     /translation="MLHLFHTTSENCSRIVTENYSTSFSSAIRMLHRDLRTPIFNIYG
                     FVRLADEIVDTFHDYDKDILLAQFKNETYAAIERGISLNPVLNSFQRTVNEFNIDLKL
                     VEAFFRSMEMDLDKKCYDCEGYKEYIYGSAEVVGLMCLYVFCEGKKEMYDQLEAPARA
                     LGAAFQKVNFLRDIKADFNGMSRVYFPGCDFSNFTEKDKREIEADIHQDFHQAYEGIL
                     RLPLKARFGVYVAYKYYLSLFKKIKRLEPARVLDTRIRIPNYLKAMIVFRAGVKNQLR
                     LI"
     gene            complement(22924..24399)
                     /gene="crtI"
                     /locus_tag="PLU11_00500"
     CDS             complement(22924..24399)
                     /gene="crtI"
                     /locus_tag="PLU11_00500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPN57610.1"
                     /translation="MSEKSVIVIGSGFAGLSSASFMAKAGWKVIVLEKNNTPGGRACQ
                     LKEQGFTFDMGPSFYWMPDVFERYFNQFGKKVSDYYKLTRLDPSYRVYWSDGYTDLPA
                     DYKALREVFESLEPGSGVMLDKYLREAAYKYEVGVNKLVYKPSQSLTEFMDWQTISGV
                     FRLEVFTSIKKHIYKYFKHSRLRQLMEFPVLFLGALPQDTPALYSLMNYADIKGGTWY
                     PEGGMYAIVDGMYRLALELGVEFRFDEAVSKIEISGTSAKKVITSKGTYTADAVISGA
                     DYQFTETKLLAPEYRSYSDRYWDKKVMAPSCLMYYVGLNKKLKSPVHHALFFDVPFDQ
                     HGVEIYKDPKWPTEPLFYVSVSSATDPSVAPEGQENIVFLVPVASGLEQDTEALREKY
                     FQIIARRMEKHLGESILDSVVYKKTFSVSDFVKEYNSFRGNAYGLANTLMQTAIFRPS
                     CQSKKVKNLFYTGQLTVPGPGVPPSLISGEVVAKQVIRTIK"
     gene            complement(24410..24910)
                     /locus_tag="PLU11_00505"
     CDS             complement(24410..24910)
                     /locus_tag="PLU11_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN57611.1"
                     /translation="MSGLEFNQLLLQNADFLKPFAVNLTRDTEAANDLYQETLYKALA
                     NREKYNVGTNIKAWLFTIMRNIFINNYRRKAKQKTIFDNTPNEYLINQKQATVSNAAE
                     SDMRIKEINAAIQGLPEIFRTPFLLYFDGYKYNEIAEVLHEPLGTIKSRIHFARKLLK
                     EQISRY"
     gene            complement(25030..25908)
                     /locus_tag="PLU11_00510"
     CDS             complement(25030..25908)
                     /locus_tag="PLU11_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPN57612.1"
                     /translation="MNAFTIKDLENLSGIKAHTIRIWEQRYHFLKPQRTGTNIRYYSN
                     DELKTVLNIALLNKYGYKISHIDKMLPEEIRSRILTLGDAQAIQERIVNELVQEMVDM
                     EIERFEKILTGYIASKGIERTVIQILFPFLEKIGILWVTGHINPAQEHLVTNIIRQKL
                     IVGIETCVSHLVKERTVLLFLPEGEHHELGLLFMYYMLKNRGIKTIYLGANVPVKDVA
                     YVVNLKKPDLVFTHLTATSSNFHLDKFLHQINTHFGSTATVVSGQLTQTYSKPVPAGI
                     TFKKSLSEVMEYLSNV"
     gene            complement(26169..26918)
                     /locus_tag="PLU11_00515"
     CDS             complement(26169..26918)
                     /locus_tag="PLU11_00515"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HPN57613.1"
                     /translation="MFKFFSRSKPEKEGDKEAIGMLKTDMHSHLIPGIDDGAPDIETS
                     LQLIGGLHALGYRKIITTPHIMWDMYKNTRETILERLEWVRTTVKEAGLDIEIHAAAE
                     YFLDDYVAGLVANNEPLLTVKDKMVLVEFSMAYPSHSLKDILFDMQMQGYQPIIAHPE
                     RYIYLEQNKAFYGELKDIGCLFQLNLLSLTGYYGKSVTELAQYLLKQNAYDLVGTDLH
                     HFRHLEALHSPALSVQLRKLLDSGKIRNAEL"
     gene            complement(26966..27628)
                     /locus_tag="PLU11_00520"
     CDS             complement(26966..27628)
                     /locus_tag="PLU11_00520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPN57614.1"
                     /translation="MYKLIKRFFDIVFSLLALIILLPVFVPIIILLLLTGEHEVFFKQ
                     ERVGFKNSIFKIWKFATMLKNSPNMGHGDMTVRRDPRITPMGHFLRQSKINELPQVIN
                     ILTGDMSFVGPRPLMKVGFDRYSEEMKSKVYNTRPGLTGIGSIVFRDEELIITNSQLP
                     PQECYRDVILPYKGALEVWYQQHQHFYTDSMILFLTAWYVVFPKSDLVYKTFPSLPKR
                     TF"
     gene            complement(27657..28805)
                     /locus_tag="PLU11_00525"
     CDS             complement(27657..28805)
                     /locus_tag="PLU11_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HPN57615.1"
                     /translation="MSNDKFIALSLPNMAGNEWKYVKDCLDTGWISSVGSYVTKFEQM
                     VADFAGAKYGIAAVNGTAALHISLLLSGVKQHDYVILPNLTFVASANSIKYLGADPLL
                     IDADPLLWQMDLDLLEEFLSTQTEVKGKELFYKKDGRRIGAIMPVHILGNMCDMDRFA
                     SIVSQYPLPVVEDATEALGTTYKGKSAGTFSPLAAFSFNGNKIISTGGGGVIVTDDEA
                     LAKHAKHLTTTAKASADEYYHDEVGYNYRLVNVLAAIGVAQMELLPSFIERKKQIVDF
                     YKKELTGVADIRFQQELPDVNTNGWLFTIQTDKQKALLDHLNANKILSRRFWMPMNKL
                     PMYKDCVYIRHIDRSDYIYNTCLSIPSSTNITDEELAIVTREIKAAIG"
     gene            complement(28798..29793)
                     /locus_tag="PLU11_00530"
     CDS             complement(28798..29793)
                     /locus_tag="PLU11_00530"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN57616.1"
                     /translation="MPKKVLLLGGAGFIGFNITKYLAENRDYELTIADNFARGKQDDY
                     FTELVNKHAIRVVAGDFTNPATFNELDEAYDQVYMLASVVGVDNANSIPHEIIRINTA
                     LIYNTLEWIRRSKVGKVLFTSTSECYAGTIEAFGYTTPTPEEVPLCIEDIGHPRFTYA
                     VTKMLGESGFLNYARILGFETTIVRYHNVYGPRMGFKHVIPHLVVRYRKGENPFKVYG
                     HDQTRAFCYISDAVEGTVLAMERPNTNGQIYHIGTQDEISIGDLIHYAGELMNFKGEF
                     ENAPTYPGSVSRRCPDITKARVQLGFEPKVMWKEGLKITVDWYNQYLESGKLVYE"
     gene            complement(30120..31631)
                     /locus_tag="PLU11_00535"
     CDS             complement(30120..31631)
                     /locus_tag="PLU11_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPN57617.1"
                     /translation="MTTSTLQVSKSPVTGKVYEIPADKKDKEGIDAFIALHSGKKVVV
                     VQGLGFVGSVMGLVVANALTEEYAVIGIDLPTPGSYWKIRSINEGVFPVIASDPKIEL
                     YYQNALQKKNYYASFDAYAYSKADVIVVDINLDVQKKSAKNKDLEGYDVDLTPFRKAI
                     EAIGAYCKEDVLVLVETTVPPGTAQKIVRPLLEEALTKRGLPVDKIKVGHSYERVMPG
                     PKYIDSIQNFYRVFAGTDEKSAVAVETFLRTVIRTDEYPLTRLGNTNATEMAKVLENS
                     YRAMNIAFMVEWSRFAEEAGVDIYEVVNAIRMRPTHKNIMLPGLGVGGYCLTKDPLLA
                     SWARMNLFGSTEKLGQSEKGVHINDKMPVYAFDFLQAQYKDSLEGKKVLLLGVSYLND
                     VGDTRYTPVEAFYDHLEMEGCAIHLHDPHVKYWEEKDIWVNQDLDGLLEDEYDVIVIT
                     TGHKDYRSNAALLEKIKNKPAAWVYDTIGVLSNEEIRSLKERHVVKVLGRGDL"
     gene            complement(31628..32305)
                     /locus_tag="PLU11_00540"
     CDS             complement(31628..32305)
                     /locus_tag="PLU11_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPN57618.1"
                     /translation="MDTNGYRVLFLRKKDNEYASRAEEFIRSNFPQATIFSGSRKDKL
                     PPEVLNWKGELLISFISSWIYPASLLESASVAAINFHPGSPEYPGTGCTNFAFYNDEK
                     EYGITCHHMKSSVDSGHIIQVKRFPILAEDTVYSVTQQCYHLIEESFYEMMDLVLKGE
                     PFPVTNEAWKRKPYTRKQLDDLCHIRPDMSPAEVDRRIRATTYKTPWAFTKIGDHIFK
                     LQAEQNQ"
     gene            complement(32732..>32988)
                     /locus_tag="PLU11_00545"
     CDS             complement(32732..>32988)
                     /locus_tag="PLU11_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPN57619.1"
                     /translation="IDHECIIGDFTHIAPGAVLCGNVKVGRNTFIGANSVIRQGIIIG
                     DNVTIGAGTVVVKDVPDGATVVGNPAKNLVKKPSRKLIAV"
BASE COUNT         8993 a         7652 c         7307 g         8936 t
ORIGIN      
        1 gcatgatcat attaaaatac aacttcatga agttgtattt taatatgatc atgcccgtct
       61 ttaataaaca ggctgtctga ccccaacgta aaatcaagca attccgcttt ttgcctgatt
      121 aacaggtaat cctgcagacg acgcatcgtt aattcaaagc ggtctgcctt cagtggtttt
      181 actaaaaaat ccaaagccga caatccgaag ctctcaaccg cataatccgg gtaagacgta
      241 acaaaaatgc aggcaggtat atcaatcagt tgctgcctga gttccaaacc actcatgccc
      301 ggcatatcta tatccagcaa taatacctcc ggtttgcttt tcttcgccag ttccaacact
      361 tcatcagccg tggaaaaaac cccggctata tgcaaaaagg ggtactgttt tgcaaaagaa
      421 accgtggtaa gtctgtctat ttcatcgtca tccgctatca cacagttaaa agttacggga
      481 ctgttcatga aagtaaattc agtttttcag gttaaaatgc ttgaaactgt caggttttct
      541 attgcttaaa aataggcttc tgcatccatt cgtctattat aaatgctgtt gggcgatcag
      601 ctttctcacc gcctaaaata ccggttaagt ttggttaatt aaaaatatat ataaaagaaa
      661 gccccaaagc cagccttttc aatcggctca ggacaacaga atgtcctgtt tgcaaaatgt
      721 atagaaggct gatatacact gacactaaac catctggcaa ctaagaaaat tactaaaaca
      781 aaaatgaaac gcattttatt atttgtactg ctgtcatcca tgttattgtt taccggctgt
      841 aaaaaaaact cttcaccggt gtccgccgta attgccaaat gtaaaccggt atctgaatat
      901 accaatttat ctggcaacgc cgccacgtat gagtatgtat atggcactga tggaaatatt
      961 tcttcgctaa aaaaatatat tggtgggggt tatcatgtac tggccgattc attactggtc
     1021 ttttatgacc atacggaacg ttttaccccg ggcattacca tgggaagttt taataaagtc
     1081 aatacagtat tcaatgccaa tatatttaca ggttctccaa caagggcaga catctctata
     1141 acccttgatg gtgtggaaca acgaaattat tacacctatt ccttttcata tgatacaaaa
     1201 aaccggcttg caaaaatcag cgagcaaaca aatacagtat taaatgacct ggaatatgat
     1261 ctttttatta tctataacga tcagaataat gttagtgagt taagatatga agccaccaca
     1321 ggaccgagaa cggttaccgt aattgctgca tccggttatg acaataaaca tactcccttt
     1381 gccggaataa taaactggcc catgctgatg catgccggct gggcaagcag cgactccgag
     1441 ccattattca ccgcactcag caaaaacaac ccgctggggt ttgagattcc gggctggaaa
     1501 agaaccatca catatgctta taacgacaac ggtttcccgg taacaagatt cagtacgaat
     1561 acaacagcat ccgctaccta ttcctttgag gaaaattttt cctgccaatg taattaacat
     1621 ctgtttcaat atttaaatca caaattatga aaaaaatatt atcaacctgt atcctgggaa
     1681 tttcgctgct gacctcctgt ggcaataatg agcaaaccca ggctatggaa gatggaaaaa
     1741 aaatggtatc cgctttgaaa gaaatgcagc caggggggat agctacatcg gtggatgggt
     1801 ggacgatgac tgcgaaattt aacgggaaac cctggacagc aaactctctt atgtccccgg
     1861 aagtggcagg aagaattgtt ggagaaaata acggggaaag tattagtctt ccgtatgaca
     1921 ggagaggaat ggttgtaggg aaaaaaacaa gattcagcca gaataatgcc gtagacctga
     1981 tgacgaacga cgaagtggcg ctgtggggtg gttacgcagg agaaatggag attactaaag
     2041 tggatgacga atgggcagaa ggcaaatttt ttgttaccgg cagttccgat tctccagcta
     2101 agcagatgaa agtcactgat ggatttttcc gcatatcact tattaaaaaa caatagctga
     2161 tccgctaaaa taagtttatg aaaacatgca tagtgattct ttctcttttc ctgttctgct
     2221 tcgacagcaa tgcccaggta ttaaaaaaat taggggagaa gataaaacgt gacagtgaat
     2281 ggagaatcag aagtaaagcc gatcagcagg tcggcagagg tatagacagt atcgttgcca
     2341 tacccaaaaa agtaaaagac aaaaagaaag caaaaaaaga ggaggcccgg aagacagaga
     2401 cccaaagtgc tgccggaaaa accgaaaacg gcagttcagg gcctttacaa acagacaaca
     2461 ggagcacaca gtcaaagctg agtgaaccgg gaaacgatga aaatgatatg gagcccagag
     2521 atggctatgc tatgcttacc ctttctgcca ataccgtatt taccggcggg agtattacaa
     2581 taagcggcga aagcataaaa tataaaaatt ataaagaggt ggaaatcacc gtcaccggcc
     2641 cttctgtaaa ggatgcaaaa tccatcccgt tgaacgcaga cggaaaattc gcaacagtat
     2701 ggtttgcctc agggaaggct ggcgagtata aggttaccct gaaaggaagc gacaaaaaag
     2761 caactgtgtc tgaagcattt actgtttatg aaatagatgg attggataac ttatgcgacg
     2821 aaaacataaa tttaattaca aaagcatttg atcatttaaa agaaacagca gagcaggtaa
     2881 aaaacagcct tggatcaaag gataaagccg aactggacaa aaagctggaa gaagtaaaag
     2941 aaaaagtcga tctattgtta aaattattaa aagacctgaa tgtagcaggt aaagatatca
     3001 gtaaactcgc caaatcagga aagaacctgt ccccgaatct tgctgacaac ctatcagttc
     3061 ttaataataa attgaaagaa cagcgggaaa agatggaaca ggtagaagaa tacaggaatc
     3121 ataagccggc cgacaatact gtttgtgaat acctggtgct gatgaatgaa gcctgtgccg
     3181 ccttttcggt atattcaaac atagagtgct ttgcttttaa aggtattatt aagaatataa
     3241 tactagacaa aggtgtacct ctggcaacgg gaacaataaa tgcaagggct aaagggttgt
     3301 cttccccaaa cgattttctt ctaaaagaac ctgcaaaaat attttcaact gcagcccttg
     3361 atgcagaatc cctgtcttcg aaattaggta cggcaggctt ttgcggggac attgtgcaat
     3421 ttgccacgga tatgttaatg aataaatatt gtggcgtttt caagggtaaa ttaaagcatg
     3481 attacacgat tgaattcaga aataaatccg ggcagaactg gtggacctat gctgttgaaa
     3541 tgaaagctgt cctggcttta cggtatccaa aggataaggg cggcgggaac atcatccgga
     3601 tgaaaggcaa cctcgaaggc aatgccacca aattcagttt ttttgaagac ctggaaaaaa
     3661 acgatgaatt ccaggaagga agcaagggca agatcgaagt tgttccaatt aaagcttata
     3721 tccctttttc cgtttcagcg gccaccagtg aaatagatgt aatggggttt ggggcaattg
     3781 caaggggttt ggccacccca gcctatttta atatcccggt ggatgcggaa tacgatgtaa
     3841 acgaaaataa aataaaacta ttcctgaatg ccccgatcat cgatttttca cccgcaatca
     3901 ggaaccagct tgtctttctt ttaattggcg gcgatctgtt gccttatgtc aaacgcatga
     3961 gtttcccgat aaacaaggtt ttcagaacat tgggttccgt agtcagggat aaccatgaat
     4021 tcccggtagt aaaagatgca aaaggcattc ttagttttga tgggaaagca aataaacata
     4081 ttggcgacag gtcatctgta atcgaaaccg atctgaattt taccatttca gcgaaaaaag
     4141 actgattact cattactcat atataccact gccccgctgt tcccgacaca ttttaccccc
     4201 gcctcctgac agcagcattc cggactgcgt tttttcaggt aagggctgct gcagttccag
     4261 caggaagtag ctgtatctgc tgacatagtt atatccatat actccagttc ttcccggaac
     4321 gatgatgccg cttctccagc ttcaatggtc gcaagaccat ccgcgccggt atagagcttc
     4381 cctacaaatt caaatcctgc tttccggatc ccttttaacg atgcctggtt ttccggcgca
     4441 tgaatgatcc agaatctttc cgcatcagga tctccctgct tcaggatata ttgaagtaat
     4501 gccggataga tgccccgtcc ccggtaagct tccagtgtcc ggaaattcca caggtacctg
     4561 tttccttccg gcaatataaa cccgtgattc agctccccga tcaccgcctg gtccctggcc
     4621 atccaaccaa aagcagcagg tatattgttt atccaggcaa caaaggcttt attgtcattg
     4681 gcaagccttt tggtcacctc ttctatgcgg gtatggctga tgattgacaa cagcaaggca
     4741 tctgtacttt ccgctatcat caatccatct gcttttttta caggactgaa atcagtcgct
     4801 ggtgaatagg tgtaaagtga catagcaatt ggttgagttt gtttgaaaat aatttacgga
     4861 atgaatgatc aggaatggca gccggcaaac gatcccttcc ctgatggaga cggatctgcc
     4921 ggatcgatga cttcaaaatt ggccatcata cctgctgcat ggtgttcgat gatatgacaa
     4981 tgatacatcc agataccggg ccggttatcc ggcatccagg caattttgat ggttgtcttg
     5041 ggtttcaggt tataggtatc cttccaggct ttataggccg gtgctttccc gttatcctcc
     5101 agcacctgga aaaagaaacc atgcaggtga aaaggatgat ccattaaact ggtattggaa
     5161 acttcccaaa cctgcagctc gcccaccatg accggtttat ccattccgtg catttcatga
     5221 ttcaccgtaa aatcaattat atgtttcaga ctgggatcca cggataactt tattttccgg
     5281 gtcacagggg catcctgtgg cgctaaagca atgatgtctg ataatttctc cggcaagtga
     5341 gccactgatt cctttctttc cagtacctgc accctggcat agggttggtt ttttgccgta
     5401 agcatggtta cccggttata cggcaatgaa cggatggaaa actgatctcc ttcgttaaag
     5461 ggaccggcaa caatatcaaa acgttcacct ggcgtgatca gccattcggt ttctatccgt
     5521 gcatactcaa gtaagttacc atcagtagca atgactttaa aaggtttacc atccaggctc
     5581 agggtgaaat accgggcact ggccgcattg ataattctcc accgttctgt ttgaccggca
     5641 tgcatatata atatggcctc ttcttttcca ttcaccaggt tggtgctccc ttcccggcca
     5701 tcatgtcttt cgatccaacg tccgactggc cccagctgtt taaattcatt atcaccggta
     5761 agtttcatat catccagcag taacagtttt tcatcgtcca ccagcaggtc agactcctct
     5821 tcaataataa tacccccata catccctctt tccatctgta tcgtttcatt ctggtgcgaa
     5881 tgataccaga aggttccggc atctggtacg gtaaagcggt attcaaaact ttcgccgggc
     5941 ataaccgggc gctgcacttc gccggtacca tccatggccg ccgggagcct gatcccatgc
     6001 caatggatca tggttggctc tggaagatta ttcaccaggt ttatcaccaa tgtatctccc
     6061 ttcctcgccc tgagtattgg acccggtacc tggttattaa atccccaggc actgatcgtc
     6121 tttcctggtt ttatttccca gttaaactca ctggcagtca ggttgtactc cactgtttta
     6181 ctttcctgtt tcatggtaat atgtttttgg ttattaaaat aaatggggtt gttccggtca
     6241 atgattacac aaaaatatcc cggcctgaag ctgagggacg gacagccgga tgtcagaaac
     6301 ggacaaatcg ttgtcaatca ttacccggta tacaagacac atattatata caggtacaga
     6361 cctgtccgtt tctgacatct gactgtctgt acaaccgtgg aatgccgtta tagctttgcc
     6421 ttatacatta accacaaaaa cataaaatga tgaaaacaaa aaccaggctg ctgcttaata
     6481 gtatcgccgc cttatctaca gccatgctgc tgtttacagt cagttcctgt aaaaaagaag
     6541 tgttctccca gggaacagta aaatcattca gcctgcaatc ggcggccaac ggggtaactt
     6601 atgacattaa agtcgccctg ccttccggct ataatcctgc agccggaaaa tatgcaacga
     6661 tctacctgct ggacggggaa gagatttttg atttcactgc caattcctgc caggagattg
     6721 ccggaaagaa tggggttgca aatgcccttg tagttagtat cgggtatggc caggaccgca
     6781 gtatcgatta cacacccacc aaaatcagca attccactgg tggtgcccct caattcctgg
     6841 attttatagc cagccagtta attccgaaaa tggaacagga ttataatgtg gataccagcc
     6901 gggccggccg ggtcatactc ggacattcgt atggtggctt attcggtgcc tacacatttt
     6961 gtgtgaacca aaaactattt ggccattacc tcctgctcag tccttctctt tggtttgaca
     7021 acctggttac cttacaactc gaaaaggaaa accgcgctct taacagaatc agtcctgccc
     7081 ttgtatttat gggtatcggt gaagcggaaa attcaggaag gatgcaggcc ccttttgagt
     7141 cattttacca atcgatacgt gatcaatacc ctgccgttac actttcaaaa aacagggaaa
     7201 aaaacctggc tcatcttggt tcacgaaacc caaacattat caaaagcctg aatttctatt
     7261 ttcaaaacag gtaattcctg cataaaatca ttccccacta tttaaaaata atttttatga
     7321 ataagcgaaa attctccatc gtcttcctcc tggtattagt ctatgccacc aattttgccc
     7381 agttgaaaat acgggtcgtc actgataaac accatacggt gcgttcattc acgatagacc
     7441 ccgccatcgg gttccatacc aatttcggat ctgatttcct gttatccacc ctggtgcagt
     7501 ggaacccgct gaaatacctc agccttgcct cgcattcctc atttgcattc aacaatatat
     7561 ttcagcggga ttttaatgga gtaaaaacaa actataacta ttccatcctg cagaaattcg
     7621 gagcgggtac aactttctat tcaagaaaaa gttcgaatac atttttactg atggcaggca
     7681 tgaaatacac ttcttttaag gaaaccatga agaaccccga caaccaaccc atcaaagcgg
     7741 ccataaactc cttcagcccg gactatggac tgatgtacgc gctacggaaa ggggtgaaaa
     7801 accgtttctt tactttccgg atgtatgtac cgctttaccc ctggccaatg aagggctccg
     7861 acatctctta tgctgacggg aatttgaaca atatagccct ggaattgggg gtcggactga
     7921 aaataaaata gtcctggaaa aatccggcac ccggtgaaca ttccccctga tgatgtaatt
     7981 tgtagtacga acggggcggt aaaaaagcag gttcgtggtc atttattcac ctcaattcta
     8041 cagttatggc atccaccaac ctgctggtaa aaaatatggt ctgccaccgc tgtatacttt
     8101 ccgttgaagg tattttgcgg caggaatcaa ttccattccg gaaagtgata ttcggagaaa
     8161 tcgaactggt cgctgattta acccctgaac agaaagaccg tctcgaagca caactggagc
     8221 aggtgggttt cgaattaatt gacaaccgca gcagcgccct gattgagaaa ctgaagcaac
     8281 tgatcataaa gagagccagg aatgaagccg gcgaaacgga taataaatta aaactctccg
     8341 cctatctttc ccgtaaggtc aatcatgagt atacttacct gagcagctta ttttcatccg
     8401 ttgaagggag aaccatcgaa aattatttta ttgaacagcg tattgaaaaa gccaaggagc
     8461 tgctgattta cggtcagctt accttatccc agattgcctt tgacctcgaa tacagcagca
     8521 ccgcccacct gtccacccag tttaaaaaga tcaccgggtt aacccccact tatttcaaag
     8581 agatcggcct tgccaagcgt aaatccatcg attccattta atcccaaaat tgtgcaacgc
     8641 atacccaacg gggtataaca gcccctcccg tacctggtct atttttgtgt tatcaattaa
     8701 catttaaaaa cataagtatg aaatcagcaa caaacgggca aatcgcccaa aacgtaaaat
     8761 gcagtaaccc taattgtacc tgtaccgatt gtaattgtgg ttctgcctgt acctgcgcca
     8821 attgtaagta atccaattca gtgcatgtgc catctgcccg ttcctttgaa ggaaagggct
     8881 tttggctgtc cgtcccgctg caaaacaatc ccggtcagcg atattcattc cctgtctgta
     8941 tctgacccta tcttgtccgt ctctactttc cgcttctgta tacctttata gtaatattct
     9001 aataatcagt gttatgcaaa aagttgcttt tattattcct cccacagttg aattgctgga
     9061 tctctccgga cctgcacagg tttttacaga agcaaaagtc ttaggaatgg aaattgagat
     9121 ggaattctat caatacagtg aggaaccggt cagtacagcc gggctgggtt ttggtaaact
     9181 ggaaaatttt aaaaccgcca ggttaaaaga aggggatttt gttattgtac cggggatgga
     9241 taatgattat gtaaacagtt tttcctttaa agcagaaagg gaatttttta actggctgaa
     9301 agaatgtgcg gataaacagg taacaatctg ctcagtttgc agcggggcat ttgccctggg
     9361 tcatgccggt ttgctgaaag atacagagtg caccacacat tggaggaggg tacaggcctt
     9421 acaaacacag ttcccgcaag cccgggtgat ggccgacatc ttatatatta aaagcaataa
     9481 tgtgtatacc agtgcgggta tcagcgccgg tattgatctg gccctggcca tactggaaga
     9541 cctgaaagga ccactgttta cccataaagt agccagggga ctggtggtgt atcaccggag
     9601 aagcggaaca cataaacaac aaagtatcta ccttgactac cggaatcata tcaatccgca
     9661 ggtacatgaa gtgcaggact acctgatcga taacctgtcc cgggaaaatg atattgaaac
     9721 actggcttcc ctggtgggta tgagttcccg caacctgagc cgggttttca aagaaaaaac
     9781 aggctccacc atactggagt acctcaccct gttgcggaaa gaatttgcca gcactatgct
     9841 gaataacccg gattacacca ttgaatatat tgcttcgaag tgtggtttta aaacagcccg
     9901 gcagttacag cggatactga aaaaataggt tccggtctac ctggttataa aatccagtca
     9961 aggtaattat cttttgatat aatagtaaat gaagcctcag gataggaacc cgtaaatgcc
    10021 ggcggtgctt ttatcttttt gtcaccggag agtttaaact catacgcgct gattttgcca
    10081 ttagtcgttt caattaaatc tacttcctgc tgattataat ttctccagaa ataatattga
    10141 acgttgtttc cctggtaagt gttccttttt attctttcag ccagcaaata gttttcccat
    10201 aatgatccta tatcatttcg taaagccggt aacctgaaat catttatgat cgcatttctt
    10261 atcccattat cataaaaata ccattttgac cctttcgaaa tttctttcct gggattagta
    10321 ctatacgcag gcagccggtg aataacaaaa acccgggaca gcagatcaag ataactctcc
    10381 actgtatttt tactcatgcc cagctgggta cccagctctg tatatgatac ttccctgccc
    10441 agctgaaaag caatcaaacg aagcagtcgt ataatcttgt cggaattccg gataccttca
    10501 tacgctaaaa tatcctttaa taaataagac tgtaccaact gttgtaaata aactgatttc
    10561 tcctggtaag atgataactg aaaaatttcc gggtaactac cgaatataag ccgttcatca
    10621 agttgctgaa cagtttccgt atacgtttct ttttccccga tttcaagttg ggcgagggga
    10681 aataaatgaa actggtattg acgccccgtt aatggatccc cggctttatt caataaatca
    10741 aatgatgacg acccggttgc tataatcgtt aacccaggca tactatcaat catcaatttc
    10801 aaaattgctc cgatctcttc aatcacctgg gcttcatcca atataaacaa agaagcatga
    10861 cctatcagtt tcctataatt ggcggctgtc ctgtttttaa ggatatcccg tatatcaaaa
    10921 tcctctgcat tcagcagtaa actttctcct ttaaacttcg ctttaatctc gtgaattaaa
    10981 actgttttcc ctacccttct ggtacccatg atcagcaata ctttattctt accgatctta
    11041 tcctctattt gcttctgaat ttttcgttta actattttca ttagttatgt cattatattg
    11101 actaaattaa tattaattta gtcatcttat tgaccaaatt aataattaaa ttacaattat
    11161 ctgttaatca ataatatata ttaaatactg cttaatgaag tctgggcgaa tgcctttggg
    11221 ccaggttcct gccgggtttt tcaaattaag tatattgaga attcttttaa agagtaaccc
    11281 agcctgctga caggttcctt atctgtttta ttatggagaa tctcaccatc attgtattcc
    11341 tgcttttcgg ggttgctttt atcggcatcc tcagtaaccg cttccggttt ccgtttccca
    11401 ttgccctggt gctttgtggt atagccatca gcctgatacc gggattaccg gtggtggagc
    11461 tggacccgga aattgttttc ctgctttttc tgcctccttt attatatgaa gcggcctgga
    11521 ataccaactg gcatgatttc aaagccaata tccggccgat cagcctggca gcaattggcc
    11581 tggtactgtt cactaccgta ctggttgccg tggtggccta ttacctggta cccggctttg
    11641 gatgggcaga atcattcctg ttgggcgcca tcgtttcccc acccgatgcc gtttcggcca
    11701 cttccgtcac caagggactt ggattaaatc cgaaaatcat caccctcctg gagggagaaa
    11761 gcctggtgaa tgatgccagc ggactggtag cttataaata cgcattggca gcggtggcga
    11821 ccgggaattt tatattctgg aaagccggac tgaatttcat gctggtggcc gccgccggta
    11881 tcagtatcgg gcttgctatc ggttacctga tgtatctcat tcataaaaaa tttatcagcg
    11941 acccggtggt ggaagtaacc ttgaccctgc tgactccctt tacatcttac cttttagctg
    12001 aacattttca tttttctgga gtactggcgg ttgtcaccac gggactctat ctttctttcc
    12061 gttccgatgt tatcctgaca caccagagcc ggatcatggg gtattcggtc tggcaggtgg
    12121 taagttttat attgaacggc ttgatctttg tacttattgg tttgcaattg cggaatatca
    12181 tgaaagggat cgggaatgaa taccagaccg gcgaactgat cctgtatggt gctatcgtca
    12241 gtctgtcggt tatcctgatc cggtttatct gggtaatccc ggccgccatg cttcaaagga
    12301 tgctgaaccg gtcgctgcgg aaaaaagaaa tatatgacac aaagaacctg gtgatttttg
    12361 gctgggccgg tatgcgggga gtagtctcta tggcggcggc gctcgccctt cccttaactt
    12421 ttccaaacgg ccaggcgttt gcgaaccgtt acctgattat attcctgacg ttttgtgtca
    12481 ttctctccac gcttgtgtta ttgggactca cgcttccgtt gctgatcaaa aagctgaaaa
    12541 caactcctta ttccattgca gaagaagaat actcgatccg cacccaggtg gtcagtgaaa
    12601 gtattaccca tatagaagaa aatctgtcac tggtaagtga cgatctcctg aataatatta
    12661 aaagtaaata cgagatcaag tacaaccggt tacaacatac ggatctgcca ccagattatt
    12721 tcggggataa gccccgtgtg cctgaaaata tatttaatgc cttcacacgt ctgcaactgg
    12781 acatgctgga gattgaaaga aaagcggtga accagttgca ccgcagcggg gccgccaacc
    12841 aggaagtgct ccggaaaata gaatacgaac ttgacctgga agaaaccagg ctgaaaatgg
    12901 aatttggatc attgggcggt tgatagtacc catcagacag ggaaagtaga tttgtaacct
    12961 ttaacacttc gccgaatgtc attacgtcaa tttatcagct cccggaagat attaaggtcc
    13021 tttatcttgc ttaaagaaaa tgtgacactt aaatggaaag aggtcgctga acttttcggc
    13081 ttacatgatg atgtatttga gaacccccgg cacctgcccc tcagcaaacc ggtgtccgtt
    13141 atcctgattg gggcgggcca ccggggcaat atttatgccg ggtatgcaca taaaaatccc
    13201 cgggaaatga aaattgccgg cgtagctgac agcaaccgga atcgcctcct gcaggccagc
    13261 aaaaagttta acctgccaga ggatcggtgt ttcagtgatt gggaagacgt ttttaaggaa
    13321 ccccggtttg ccgatgcggt catcatagcc accccagaca atctgcatac cgccccttgt
    13381 ctgaaagcgc tggagatggg ttatgatatt ctgttggaaa aacccattgc ccctactgaa
    13441 gaagaatgcc ggctgatatt agccaaagcc aggtcgcata accggattgt cggggtttgt
    13501 catgtattgc ggtactcccc ttatttccgg gaactgaaag aagttctgga tgccgggctg
    13561 atcggaaata tcatcagcgt acagcatatg gagcccatag aacaaattca tatgagtcac
    13621 tcgtatgtaa ggggcgtctg gaaaaacagt aagaccgcca ccccgattgt actggctaag
    13681 tccagtcatg atacagatat catccgttgg ctggtaaaca gcccggccaa agatgtgcat
    13741 tgttttggca acctgaaatg gtttaccaat gtaaatgcgc cggaaggcag cactgaaagg
    13801 tgtacagacg gatgcaaggt tgaaaaaagt tgtgcttact ccgccttaca gatatatctc
    13861 cgggaccgga aaaggcttta tgtttttgat atgccgagta acaaatcgct ttgggatgat
    13921 tttattctta cccagctccg gacaactgat tacggccgct gtgtatatag aatggataac
    13981 gatcagccgg atcacctgac ggtaaatatg ttgtttgaaa acggggtgac ggccgccttt
    14041 tccatggagg cccatgtatc ttatgaaggg cgacgtacac ggatcatggg ttcaaaaggg
    14101 gatatagtgg gtgatatgga aaactttgtg ctgactgatt ttaaaacacg gaaagtaaca
    14161 tcatgggaaa tgaaaaccga tgcacacggt ggcggagatc accggttagt gaaagattgg
    14221 ctccaggcgg tggcgcagca ggataaatca atcttaagtt cttccatcga agtgtctatt
    14281 gaaagtcacc tgatggcttt tgcagcggaa aaaagcaggc ttaaccggac tattgaagaa
    14341 ataaacctgg atcttgataa agctacttac tgaaatattt aacaaagctg ttcagcaatg
    14401 ccgtccggac catatcctcc cgggcatgct agaactgact gctgatgaac tgaaaattga
    14461 ttcgtatagc tgtaaacggg agcaggtaag ggatttttat attattgccg ccggtaaagc
    14521 agcgggtgcg atggcagcag aggccgaaaa ggtatttggc gcatttataa ccggaggcct
    14581 ctgtattacc aagtacgatc atgccctgcc gttgcaacgg ctgaaaatgc tggaagccgc
    14641 tcaccccgta cccgatgaaa atagtgtgga ggccggcaga caggtacttc aattgctgaa
    14701 aggccggtca gaaaacgaca tagttctttt cctgatatca ggaggagcgt cttccctggt
    14761 aacagacctg ccgtcgggaa ccagcattac cgatctgaca aaaagttacc agctgctggt
    14821 aaacagcgga gccgccattg atgaaataaa ctgtgtccgg aaacacctga gccggattaa
    14881 agggggccaa ctggccaaag cggcttaccc ggcccgcatt atcagcctgc tgatcagtga
    14941 tgttccgggt gatgatctgc agacaatcgg cagcgggcca actgttcccg atcactcaac
    15001 atatgaggaa gcgctgagcg tagtaactaa atacgggctg caggaatcac ttcccgcttc
    15061 aatatggcag catttaaata tgggagtgca gggagcgctg gaagaaacgc ccaagccagg
    15121 tgatccggta ttccgttccg tttatacccg aataatcgcc aataattaca aagcagcagc
    15181 cgccgcaaaa atggaggcgg agaaatccgg gtttcatgta cagtgggata aacggctgat
    15241 gaccggaaat acggatgcgg aagcacagga attttcgcag caactgctgc actatgaagg
    15301 ccctttgccc gctttgttta tccggggagg cgaaacgacg ctccgggtaa gcgggaatgg
    15361 taaagggggg cggaaccagc attttgcgct gagagccctt ctggaaatga aaacccattg
    15421 gaataaaccc tccggatttg ccatcctcag tgcaggaacg gatggcacag atggcccgac
    15481 agatgcagcc ggggcgatca ttgattcagt aatgctgacc aacccggatc tttcagtaac
    15541 taccatacag acacacctga ctcattttaa tgcgtatccg ttttttgttc aaaccggtgg
    15601 tctacttatc accggaccca cacagacgaa tgtaatggat atggtgatag gtatgatttt
    15661 ttaaatatac agaaacaact acatcggtgt agtgtcatcg ctacagcccc ttataaaata
    15721 ctacaacctg catcatcctt attactgaca caggtcagtt ttcattgaca aactaaaaga
    15781 aatacgcagg cctgattagg gatacactta aacatcaata aacggttgac tgctttatac
    15841 ttcgctaatt taaattccgt tttatagata caactaaaaa aaaatccgtt aaagtatctt
    15901 ttgaaatgtt gaatcaaatc aagtttaaaa aaaaatctgg cattgatttt ggataacaac
    15961 gatgctttac cggcacactt tatttaatca ctcagccgga aaaaaaggaa cgaagaaaac
    16021 aaaggttaag gcaattaagg aacgaaccac taacaccgga aactgtacca cttcacagta
    16081 cccggtcaac cactaacaca tacgaacatc cttccctgta caggtccata tctgtcagat
    16141 acgccttcta ttgcccgcgt tttcctgcct gctttttaca accaatatcc aaccggcgta
    16201 attttttttt acaactaaaa aacgtacaaa atgaaaaacc agtccaggca tctttcgctt
    16261 gcccatccag aaaaaaaatc aatgctcacc ttagtgacga ttgccacctt actgatcggt
    16321 gtgttcctga tggcctcctg ccggaaatca acagacatgc cctttaccgg cgatgaaccc
    16381 gtgagtaaca cagacagtgt atctgtcatg agagaagcag ccgcattggc cgtaaacact
    16441 tattacctct ccccttccgg gaatgataac aattccggca gcattgacca accctggttc
    16501 acgcttcaga aagcatggac cgtactcgct gctggcgacc tgttatattt gaggggtggt
    16561 aagtatacgt acaccgttca gccttacctg accgggaaaa gcggtaccgc cggcaacctg
    16621 ataaaagtct gggcctatcc cggtgaatca cccgttatca cccgtggtac ttcctttaat
    16681 aaatcgaatg gctggcaccg ggggatggtc tatctgtccg gtaattacat tcacttaaaa
    16741 gggatccgtt ttacaggcat gtatacggat gacaaccagg tggatgccgg tctgcaaacc
    16801 tataatgtga ataattgtgt attcgagtta ctggaatgcg ataataatgt ggaaggaatg
    16861 atcgtagaaa ataacagtaa caataacctg atcctgaata gtgatttcca tgacaacttc
    16921 agtaattatg gcggctcgaa cggaggcaat tctgatggcc ttggtttaac ctatatgatc
    16981 gggcagaaca ccaccaccac ggttcgcgga tgccgcgcct ggaataacgg cgatgacggc
    17041 ttcgatactt ttgaaaacag cggttatgtg ctgattgatt cctgctggtc ctggcataat
    17101 gggtataata aaggaaccaa tacctcagcc ggagacggtg tcggatttaa actgggttca
    17161 gatttcctga ccaccccagc aggtgtgggt gtggtaaaaa gaagactgca acgtagtctt
    17221 ggttttgata accgtaatgc gggagcccat atcaatgaag cagatttcag caccgaaatc
    17281 ttcaataacg tttttttccg gaacaaaatc accggactga acttccatta caataaccgg
    17341 gttcacactt tccgtaataa tgtatccttt ggcaataccg acaaagatgt tgaaatcagc
    17401 ggaaacagta tccgtagtaa taattcagca ggtaccgccg gtaatgatgg tggctggaca
    17461 acgaatgcct ccgcggctga cttcctgaat acggacactt ctggtatccg gaatgcccgt
    17521 caggccaatg gttcacttcc aaacattaat ttcctgaaac tcgcagccgg ttccgatctg
    17581 attgatgccg gtatgaatgt aggcctgtat ttcaccggga ataatccgga ccgtgctgcc
    17641 cttgaatatg ggggtgtgtc ggtacccgtt gttaacctgc ctcctgttgt aaatgcaggg
    17701 gtggataaaa ccattaccct tccggtaaac aatgtaaccc tgagtggttc ggccactgat
    17761 gcagacggaa gcattgcagt ctatgcatgg acaagaacca gtggtccttc cacaccgacc
    17821 ctgagtgggg ccaacacgtc cagcttatcg gccagcaacc tggttgcagg aacctatctt
    17881 ttcagactta cggccaccga caataacggg gctaccgcat gggatgaaac cgtggtcatt
    17941 gtggccggtt ccggtacacc cgctaatctg gcacctactg ccaatgcagg aaccgataaa
    18001 acaatcgttc tgccggttaa cagtatagca ctcaccggta gcggaacaga ccctgacggt
    18061 acgattgcat cttatgcatg gacttaccgt agtggtccgt caacaccggt acttaccggt
    18121 gcaacaaccg ccaacgtaac agccggcagc ctggtggcag gtacgtatgt acttcgcctt
    18181 accgtgaagg ataatggagg tctcagcgga tttgatgatg tcaatatcat tgtatcggct
    18241 tctactacta ccaatcttcc tccggtagcg aatgcaggtc ctgataaaac gattaccctg
    18301 cccactaata ccgttacctt ccttggcagt gcaaccgatc ctgatggcac aatcgcctcc
    18361 tataactgga cctatcgcac cggaccagcc accccatcac tgaccggggc aaggaccgcc
    18421 actttaaaag ccagcaatct gaaagcggga acgtatacct tccgtcttac cgttaaagac
    18481 aataaaggaa aagctgcctt tgacgaagta aatgtcatcg taaaagcagc tgccagcacc
    18541 ggtggcacca aacccatctc cgtatcgaat gcggtagcgg atgcaggcta ttcctattat
    18601 gtggttcagg atttcggcat tctgcccgat gatggtgcaa atccggtccg ttccaccctc
    18661 cgtatttttg aaaacggggt ggaattaaat cctgcccatt ccacccacta tgatatccag
    18721 accattggca gaggacgttt cagccattgg ggtgatggtt cctttatcgt attgtatttc
    18781 tctgcctcag ataatacgaa tccaaaaaca aacggcagaa catacaccta tactactgtt
    18841 cagtaaaagc aaaactaact gaacctgaaa tccgccccat gggggcggat ttttttttga
    18901 acttcccggt acccctcccg atgtttcctg attcccaact ttttcttacc tttctgtata
    18961 tcaaaaccta aaacatgcct gcgccttcca caaaagcctc cacgttctta ttattcccct
    19021 ggggattatt atccatcgta ctggtacttg gcctgaatct cgtattggat aatgactggt
    19081 tcagcaagaa ctggagctgg attgcactcg tactgattat tcctgccgct atcctggaaa
    19141 attttaaagt aggttactcc accatgcgga tcatgtttat gaaagtggtg tactccctga
    19201 tctccttcct gcttgtgggc atggctttcg gttccgccct gaatggctgg catatcgccc
    19261 gttttcagaa aatcgctgat gtaaaaacca tcctccttcc ctggtatgtg gtgctggccc
    19321 tgtcgatatt gctcctgata caggtggcac aattgttcag tatgctgaaa gccgtgaagc
    19381 gggagtttat gcagatggac cctgaccagt agttagtagt cggtagtcgg gagttagtag
    19441 cctgcattta tcagaagaaa gtgaggtgtt gccaggcagt tggcagttgg cagttggcag
    19501 ttggcagttt ggaatttttt cttatgtgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnng gcagttggca gttggcagtt ggcagttggc agtttggaat tttttcttat
    19681 gtgttgagtt gcttcttctt taagaaaata attaaataat caataattaa taattttcat
    19741 tcgttcgttc tcttcccgct tttctctatt ctttcttttt ctcctttgac ctctttagcg
    19801 ccacgcaggg aaaattgggt ttagctgttt attcgtttga tgtttagcgc aactgcatca
    19861 acgctgcttt taaaaaaggc caggtaggta aagaagagat aatccccaac tcctcccgca
    19921 gtgtactttc gttatccaga aacttcagca cctgggtggc tttgtttttc ttaaagaggg
    19981 tggagaatat tttccggccc ggaagggttt tatgatagag gatatagagc agggtattgt
    20041 cgtaaaaacg gaaacgccag ggtgtgggtt tcaggtgaga cagtgattcc cccttcagca
    20101 acagttccag aatcctatca gactgcttct gaataaactg aaaagtatag ccgctcgaag
    20161 ccttggtttg tccgccggcg gtaccgatat gaatcacccc gttctgctga atgggaaatt
    20221 tttcatccgt cattggaatg atgccgaact cctcttcttt cacggtgtat tgagtaatct
    20281 tcaaaaaatc acggatataa tttttcaact cctggtcata ttgttccgat gccagcaatt
    20341 gcggggtaaa caacgtgtat tccacgaggg ccttatgcgc atcgaaaggc aatacatagg
    20401 cgaatgtggt gccttcctgc tggtgcaccc ggaaatccat cagggaagct ttgccgggat
    20461 caaatacggg ctgctcggtt tcaatcaccc aacctttgaa atgctgtaaa acatttaacc
    20521 gggatgtacc cggacgatag actgcattaa atacgactgc cgggggcagt tggaattgcg
    20581 cctgattcaa taccagggta gtactctttt catcggtgct gcatccgccg atttcagcat
    20641 agatgatttc tatttgcgga tgccggctga tttcctgaaa acaatactgg tagaagtcag
    20701 ctccccggat cattttgtac tcgtaggggg aaatagtaag ggtggcctga tacgtatcac
    20761 tgagaaaatc cacccttggc catttccggt aaacgatgga ttcaaaaaaa ctatcgcccg
    20821 cttcccagaa gcaccaggta cggtcgtttt ttgtcttcgg ttcccggtcg atcaggagaa
    20881 tttttttatc tgagaatcta ccgctgcgga tcatgcgcag gagcagactg agcgaggcac
    20941 agccggcacc cagaaaaata taatcatatg atgattgctt gaacaggact gcaaattaag
    21001 cttcttctgc ttccggagca tattccacgc ggcgggcacc ggccatcagc tgcttatcgc
    21061 gttgcacctt ttgccagtat ttccgggcga catacagaaa gccaaaactt tcaccttgtt
    21121 ccttattcag gtgcttatgg tgcattttat gtgcccagcg gatggcgcgt acataggtat
    21181 tattgctgcg gctgaaccac ttgaagcgct ggtgaatgat cacatcatgt accaggaaat
    21241 aagcggcgcc ataaagcatg attccaaaac caatggattg catccaccag acatgatcat
    21301 aggtgccaaa ataaatcagg aggaaactcg gcacggcaaa aatgaacgca aagagatcgt
    21361 ttttttcaaa cacacccggg cgtacctggt gatgatcctt gtgcaggatc cagagaaaac
    21421 cgtgcatcac atatttgtgg gtgcaccagg taataccttc catgacgaaa aaggtgccga
    21481 agaaaacgag aatgtaaaga agaatatcca tgttcttgaa aaattaatca gttaaggccg
    21541 gggtactgta ctgttgttgc caccagcttt caattttggg aaaggcgagc tgaaaccagg
    21601 ctgtgtataa ccccggccgg gtttgcaggg attcagcaat ggcgggcatc ggtttaaagc
    21661 aataatccat gacctcatcg ggattgcagc gtatttcacc ctcatattcc ccgctgtata
    21721 catggtcaaa ctcatattcc gtcaggccat tgtcaaattc ggccttatat acaaaatcaa
    21781 ataatttctt tgccggggta tcaaatccca tttcctccac cagcctgcga tgtacggcat
    21841 catccgtatt ctcgcccatc gccgggtgac tgcaacaggc gttggtccac aggccgccgc
    21901 tatggtattt attccgcgcc cgttgctgca ggagcatttc acctttgcgg ttaaagacga
    21961 atacactgaa agcccggtgc agaaagcctt tccggtgtgc ctccagcttt tcacaagagc
    22021 ccaggatttg atcctgctca tccacgagaa ttacttgggt aaggtccata tcagatcagt
    22081 cgaagctggt ttttaacgcc tgccctgaat acgatcatcg ctttcaggta atttggaata
    22141 cggatccgcg tgtccagtac acgggccggt tccaggcgtt ttattttttt gaaaagggag
    22201 agataatatt tataagccac atacacgcca aaacgtgctt tcaggggcaa ccggagaata
    22261 ccttcataag cctggtgaaa atcctggtga atatcggctt caatttcccg tttgtctttt
    22321 tcagtaaaat tggaaaaatc acagccgggg aagtacacac gggacatccc gttgaagtcg
    22381 gccttgatat cgcgcaggaa attcactttc tgaaaggctg ccccgagtgc ccgggccggt
    22441 gcttccagct gatcatacat ttcctttttg ccttcacaaa atacatagag gcacatcagt
    22501 cccaccactt cagcagaacc atagatatat tccttgtatc cttcacagtc gtaacacttt
    22561 ttatccaggt ccatttccat actccggaaa aaggcttcca cgagtttcag atcaatattg
    22621 aattcattca cggtacgctg gaaactgttc agcaccggat tcaggctgat gccgcgttca
    22681 atggcggcat aggtttcgtt tttaaactgc gccagcagga tgtctttatc ataatcatgg
    22741 aatgtatcca caatttcatc cgccagacgt acaaacccgt agatattgaa tatcggggtc
    22801 cgcaaatcac ggtgaagcat gcggatcgcc gaggagaagg aggtgctgta attctccgtt
    22861 acaatccggc tgcaattttc actggtggta tgaaaaagat gtagcatact tcaatataag
    22921 tatttattta attgttctga tcacttgttt agccaccact tcaccgctga tcagtgaggg
    22981 tggaacaccc ggaccgggaa cggtcagctg cccggtataa aatagatttt ttaccttttt
    23041 actctggcag gatggccgga aaattgcggt ctgcatcagg gtgttggcga gtccgtaggc
    23101 attaccccgg aacgaattat actcctttac aaaatcactg acagaaaatg tttttttgta
    23161 caccactgaa tcaagtatgg attcacccag gtgtttttcc atccggcggg cgattatttg
    23221 aaaatatttt tcccgcaatg cttcggtatc ctgttctaga ccggaagcta ccggaaccag
    23281 gaatactatg ttctcctgtc cttccggtgc cactgacgga tctgtagcag atgaaacact
    23341 tacatagaac aaaggttcag tgggccactt cggatcttta taaatttcca ccccgtgctg
    23401 atcaaaaggc acatcaaaaa acagggcatg gtgtaccgga cttttcagtt ttttattgag
    23461 ccccacataa tacatcaggc aggaaggggc cattactttt ttatcccagt aacggtcgga
    23521 ataagaccgg tattccggcg caagtaattt tgtttcggta aactggtaat ccgccccgct
    23581 gatcaccgca tcggccgtat aggttccttt actggttatt acttttttgg cggatgtgcc
    23641 gctgatttca attttactga cggcttcatc aaacctgaat tccacgccca gttcgagggc
    23701 cagccggtac atgccgtcca caatcgcata catgccgcct tccgggtacc aggtacctcc
    23761 tttgatatct gcataattca tcaggctgta aagagccggt gtatcctgtg gaagggctcc
    23821 caggaaaagc accggaaatt ccatcagctg acgaagtctg gaatgtttga aatatttata
    23881 aatatgcttt ttaatggacg taaacacttc gagccggaat accccgctga tggtttgcca
    23941 atccataaat tccgtcagtg actggctggg tttatatacc agcttattca ccccgacttc
    24001 atatttataa gccgcttccc ggagatattt atccagcatg accccgctgc ctggttccag
    24061 tgattcaaaa acttcccgga gtgctttata atcagcgggc agatcggtat agccgtctga
    24121 ccagtatacc cggtatgaag gatcgaggcg ggttagttta taatagtcgg atactttttt
    24181 tccaaactga ttgaagtagc gttcaaatac atccggcatc cagtaaaagc tgggacccat
    24241 atcgaaggtg aaaccctgct cctttaactg gcaggcgcgt cccccgggtg tattgttttt
    24301 ttccagtaca ataactttcc agcctgcttt ggccataaaa gatgccgagg acagaccggc
    24361 aaatccgcta ccgataacaa tgactgattt ttccgacaaa tgaagtgatt taataacgac
    24421 tgatctgttc ctttaataat ttacgggcaa agtgaatacg gcttttgatc gtacccaggg
    24481 gctcatgcaa tacctcggcg atttcattgt atttatatcc gtcaaagtac aacaaaaaag
    24541 gagtccggaa gatttcaggt aatccctgga tggccgcgtt gatctctttg atccgcatgt
    24601 cgctttcggc cgcattgctg acggttgcct gtttctgatt gatcaggtat tcattcggcg
    24661 tgttgtcgaa aatggttttt tgtttagctt ttctgcgata attattaata aaaatattcc
    24721 gcatgatggt aaacaaccag gccttgatat tcgtgcctac attatatttt tcacggttag
    24781 ccagggcctt gtacaatgtc tcctggtaga gatcatttgc ggcttctgta tcacgggtga
    24841 gattcacagc aaaaggcttc agaaagtctg cattctgcag cagcaactgg ttaaattcta
    24901 atccggacat ataattgtgt ttaattatta tacggtaaaa ttaaacattt cggattgatt
    24961 ttaagaatat ttttgtttaa ttaaaatgtt aaaaaattaa acactagtaa tcagtcagtt
    25021 agcctgttat tatacatttg aaaggtattc catgacctca gaaagtgatt ttttgaaggt
    25081 aattccggca ggaaccggct tggaataagt ctgggtaagc tgcccggaaa ccacggtggc
    25141 cgtgcttcca aaatgggtat tgatctggtg caggaattta tccaggtgga agttggagga
    25201 agtggcagta agatgggtaa ataccagatc cggttttttc aggttcacca cataggccac
    25261 atcttttacc ggtacattgg caccgagata gatggttttt atcccccggt tcttcagcat
    25321 ataatacata aataaaagac cgagttcatg gtgttctcct tccggcaaaa acaataatac
    25381 cgtccgctct ttgaccaggt gtgatacaca ggtttcaata cccacgatca gtttctgacg
    25441 gataatattg gtaaccaggt gctcctgggc aggattgata tgaccggtta cccagagaat
    25501 accgatcttc tcgagaaagg gaaagagaat ctggatcacc gtccgttcaa ttcctttaga
    25561 cgcaatataa ccggtaagta ttttttcaaa acgctcaatc tccatatcca ccatttcctg
    25621 caccagttca ttcacaattc tttcctggat cgcctgcgca tctcccaatg taagaatacg
    25681 gctacggatc tcctccggca gcattttatc aatatgggaa atcttatacc cgtatttatt
    25741 cagcagggca atattgagca ccgtcttcag ctcatcattg ctgtaatacc ggatattggt
    25801 gcccgttcgc tgtggtttca gaaaatgata ccgctgctcc cagatacgga tagtatgggc
    25861 cttgatgccg gacaggtttt cgaggtcttt tattgtaaac gcgttcatga atatagatac
    25921 aatcgggaaa ggaaaaggtt gacggggaga gaaagtcaaa agtcaaaagt aaaaagggtc
    25981 gcataaaagg gtagattctt aactttttca ggaaatcagg ctgttgcagt aatttgattc
    26041 attccctgct ttcagttttt ttacttttta cttttgactt tttacttttt acttcccaga
    26101 aaatctgaca cccttatgaa gttaatgatt tcaggctttt tcttgctgac tgtgaagtcc
    26161 ggtgaaagct acaactctgc attacggatc ttcccgctat ccagcagctt ccttaattgt
    26221 accgacaaag ccgggctgtg aagggcttcg aggtgacgga agtgatgtag gtctgtacct
    26281 accaggtcgt aggcgttttg cttcaacagg tattgagcca gttccgttac ggatttacca
    26341 tagtaaccgg tcagggagag caggttgagc tggaagaggc aaccgatatc ttttaattcg
    26401 ccatagaagg ctttgttttg ctccaggtaa atataccgtt cggggtgggc gatgatgggc
    26461 tggtaaccct gcatctgcat atcaaacaga atatctttca ggctatgcga aggataggcc
    26521 atggaaaatt ccaccagcac cattttatct ttcaccgtca gcaggggttc gttattggcc
    26581 accagtccgg ctacataatc atccaggaaa tattcggcgg ccgcatggat ttctatatcc
    26641 agaccggctt ccttcaccgt ggtgcgtacc cactccagtc tttccagaat cgtttcgcgg
    26701 gtattcttat acatatccca catgatatgc ggggtggtaa tgattttccg gtaacccagt
    26761 gcatgcagac cgccaatcag ctggagggaa gtttcgatat ccggcgcccc gtcatcaata
    26821 cccgggatca ggtgcgagtg catatccgtt ttcagcatac caatcgcctc tttgtcccct
    26881 tccttctccg gcttcgatct tgaaaaaaat ttaaacatgc tgacccgctt tgtcgtcgaa
    26941 attacaaatg aatttgatca tcctttcaga aagttcgctt cggaagggac ggaaatgttt
    27001 tatatacgag atcactcttg ggaaaaacca cataccaggc ggtcaggaac aggatcatac
    27061 tatccgtata aaaatgctgg tgctgctggt accagacctc caacgcacct ttatagggca
    27121 ggatcacatc ccggtaacac tcctgtggcg gcagttgact gttggtgatg atcagttcct
    27181 catccctgaa tacaatggac ccgataccgg ttagccccgg ccgggtattg tacactttgg
    27241 atttcatttc ctccgagtaa cggtcaaacc cgaccttcat caacggacgg ggaccaacaa
    27301 atgacatatc acccgtcagg atattaatga cctgcggcaa ttcgttgatc ttactttggc
    27361 ggagaaaatg acccatcggt gtaatacgcg ggtccctgcg taccgtcata tccccatgcc
    27421 ccatattggg actattctta agcatggtgg caaacttcca tattttgaaa atggaatttt
    27481 tgaatcctac ccgctcctgc ttgaaaaaaa cttcatgttc tccggtcagc aataaaagaa
    27541 taatgatcgg gacaaatacc ggcagcagaa tgatcagcgc caataaggag aatacaatat
    27601 caaaaaagcg tttaatcagt ttgtacatac aaaaagagtt aaggcagtaa caccggttac
    27661 ccgatcgcgg cttttatttc ccgggtcaca atggccagtt cctcatcagt gatattggtg
    27721 gaactcggta tactgagaca ggtgttgtaa atataatctg accggtctat gtgccggata
    27781 tacacgcagt ccttatacat cggcagttta ttcatcggca tccagaagcg gcggctcagt
    27841 atcttattgg cattgagatg atccagcaag gccttttgtt tatccgtctg aatggtaaag
    27901 agccagccat tggtattcac gtccggcagt tcctgctgga accggatatc ggccacgccg
    27961 gtgagttctt ttttataaaa atcaacgatc tgcttttttc gttcaataaa ggaaggcagt
    28021 aattccatct gtgccacacc gatggctgcc agtacattca ccaaccggta attatacccc
    28081 acttcgtcat gataatactc atccgcactg gctttggccg tggtggtcag gtgcttggcg
    28141 tgtttagcca gggcctcatc atccgttacg atcacgccgc caccgccggt ggagataatt
    28201 ttatttccat tgaaactgaa agctgccaac ggactaaaag tgcccgcgct tttcccttta
    28261 tacgtggtgc ctaatgcctc ggtggcatcc tccacaacag gcagcgggta ttgactgaca
    28321 atcgacgcaa aacggtccat atcgcacata ttaccgagga tatgcacggg catgatggct
    28381 cctatgcggc gaccatcttt tttatagaat aattccttcc cttttacttc agtctgtgtg
    28441 gacagaaact cctccagcag atccaggtcc atctgccata gcagcggatc ggcatcgatc
    28501 agcagcgggt cggcaccgag gtatttgatg gaattggccg aagctacaaa tgtcaggttg
    28561 ggcaggatca cataatcatg ctgcttcact cccgatagca gtaacgagat atgaagggcc
    28621 gctgtcccat tcaccgcggc aatcccatat ttagccccgg caaaatcagc gaccatctgt
    28681 tcaaatttgg tcacatagga acctacagag gaaatccacc cggtatccag gcagtccttc
    28741 acatacttcc attcattccc tgccatattg ggcaggctca gggctataaa cttatcatta
    28801 ctcataaacc agttttccgg attcaagata ttggttgtac cagtccacag tgatcttcag
    28861 tccttccttc cacattactt taggttcaaa gcccagttgc acccgggcct tggtaatatc
    28921 gggacaacga cggctgaccg aaccgggata ggttggcgcg ttttcaaatt cgcctttgaa
    28981 attcatcagt tccccggcat aatggatcag atcgcctatc gaaatttcgt cctgggtacc
    29041 gatatgataa atctgtccgt tggtattggg acgctccatt gccaaaacgg ttccttccac
    29101 ggcatctgaa atataacaga aggcacgggt ctgatcatgc ccatatactt taaacggatt
    29161 ctcccccttg cggtaacgta ccaccaggtg agggatcacg tgtttgaagc ccatacgcgg
    29221 accgtacaca ttatgataac ggacaatggt agtttcaaat ccgaggatgc gggcatagtt
    29281 caggaaaccg gattcaccca gcatcttggt cacggcatag gtaaagcggg gatgaccgat
    29341 atcctcgata cacagcggca cttcttccgg cgtgggagtg gtatacccaa atgcttcaat
    29401 ggtacccgca taacattcgc tggtggaagt aaataatact ttgcccactt ttgaacggcg
    29461 gatccattcc agggtattat aaataagggc cgtattgatc cggatgattt cgtgcggaat
    29521 ggaattggca ttgtctaccc ccactaccga agccagcatg tatacctggt cataggcttc
    29581 atccagttca ttgaaggtag cgggattagt gaaatcaccg gccactacac ggatcgcatg
    29641 tttattcacc agttcagtaa aataatcatc ctgtttaccc cgcgcaaaat tatccgcaat
    29701 cgtcagttca tagtcgcggt tttccgccag gtatttggtg atgttgaatc cgatgaatcc
    29761 ggctccgccg aggagcaaca ctttttttgg catttttcta ttattaagtt tattttcttt
    29821 attgttaaat gagaattgag catttcgctt ctttggaatt caattttcaa tactcaacgc
    29881 tcaattttca attttcattt gttgaaccca cttcggggtt caggaaacca tccttattct
    29941 ttaccccggg tttcacccgg ggctattaat cgttcaaccc tttcagggtt ggcagattca
    30001 ccttactatg atgaatcttc gttcaattgt taaatgagca tttcacggct gcaaccgatt
    30061 cccgtacttc gtacttcgta cttcgtacct cgtacttcgt acctcgtacc tcgtactttc
    30121 tacaaatcac ctctccccaa cactttcacc acatgccgtt ccttcaatga tctgatttct
    30181 tcattgctca gtacaccgat ggtatcgtaa acccaggcag caggtttgtt tttaatcttt
    30241 tccagcaagg ccgcattgct gcggtaatct ttgtgcccgg tggtgatgac gatcacgtca
    30301 tattcatctt ccagtaagcc gtcgagatcc tggttgaccc agatatcttt ttcctcccag
    30361 tatttcacat ggggatcgtg caggtgaatg gcacagcctt ccatttccag gtgatcgtag
    30421 aacgcttcca cgggcgtata acgggtatcg cccacatcgt taagataact tacacccagc
    30481 aacagtactt ttttcccttc cagtgaatct ttgtattggg cttgcaggaa atcaaacgcg
    30541 tataccggca ttttatcatt gatgtgcacg cctttttcac tctgtcccag tttctccgtg
    30601 ctgccaaaca aattcatccg ggcccagctg gccagcaagg gatccttggt gaggcaatag
    30661 ccacctacac cgagaccggg taacataata tttttatgcg tgggccgcat gcggatggca
    30721 ttcacaactt catagatgtc cacaccggct tcttccgcaa aacggctcca ttccaccatg
    30781 aatgcaatat tcatggcacg gtaggaattc tccagcactt tcgccatttc agtggcattg
    30841 gtattaccca ggcgggtcag cggatattca tccgtgcgta tcacagtacg caggaaggtt
    30901 tccacggcta ccgcactttt ctcatcggta ccggcaaaca cccggtaaaa attctggatc
    30961 gaatcaatgt atttgggacc gggcatcacc cgttcatagg agtggcccac ttttattttg
    31021 tcaacgggaa gtccgcgttt ggtcagcgct tcttccagca ggggacgaac aattttctgg
    31081 gcggtgccgg gaggaaccgt tgtttccacc aacacgagta cgtcttcttt acaatacgcc
    31141 ccgatggctt caatggcttt gcggaagggg gtcaggtcga catcatatcc ttcgaggtcc
    31201 ttgttctttg ctgatttctt ctgcacatcc aggttgatat ccaccacaat cacatcggcc
    31261 ttgctatagg cataggcatc gaagctggca taatagtttt tcttttgcag agcgttctgg
    31321 taatacagtt caattttcgg atcgctggca atcaccggga atacgccttc attgatggaa
    31381 cgtatcttcc agtaagaacc cggggtagga agatcaatac cgatcaccgc atattcttcc
    31441 gtgagggcat tggccaccac gagacccatc acagaaccca caaagccaag gccctgcacc
    31501 accactactt tttttccaga atggagggcg atgaatgcat caatcccttc tttatctttt
    31561 ttgtcagcgg gtatctcata taccttaccc gttaccgggc ttttggatac ctgcagcgtg
    31621 ctggttgtca ttggttctgt tccgcttgaa gtttaaaaat atggtccccg atctttgtaa
    31681 aagcccaggg ggttttatac gttgttgccc ggatccgtct gtcaacttcg gccggggaca
    31741 tgtccggccg gatatggcaa agatcatcga gttgttttct cgtgtacggt tttcttttcc
    31801 acgcctcgtt agtaaccgga aatggttcgc cttttaaaac gagatccatc atttcataga
    31861 aactttcttc gatcaggtga taacactgct gtgttactga gtaaacagta tcctccgcaa
    31921 ggatgggaaa gcgttttacc tgtataatat gaccggaatc cacgctggat ttcatgtgat
    31981 gacaggtgat tccgtattcc ttttcgtcgt tatagaaagc aaaattggta caaccggtgc
    32041 cggggtattc gggactgccc ggatgaaaat taatggcggc caccgaagcg ctttccagta
    32101 agctggccgg gtagatccag gaagaaataa aagagatcag gagttcgcct ttccagttca
    32161 gaacttccgg gggcagtttg tctttgcggc tgccggaaaa aatcgtggcc tgtggaaaat
    32221 tggagcggat aaattcctcg gcccggctgg catattcgtt atcctttttg cgaaggaata
    32281 agacacggta tccgttggta tccacaggtt tcaagaagcg gggtttaagg ctgtttttta
    32341 aagggcaaat gtactgagaa aagtcgagaa aggcggcccc ctaaagggga ctttgcctgc
    32401 ggcaaaagag ggggccacga attaacacga aatcacacga aaggccacta aaggatttgc
    32461 tgcgcaaatg aatgcctgcg gcatttgagc cacggatctc acggatagcc acggattttt
    32521 gatttccgta ctcccgattg atcttttttt tgccacgaaa acacacaaaa tcacacgaaa
    32581 aatacaatag ttctttgcgg agatttttta atccgtgttt atcagtgtga tccgtggcct
    32641 gcctttctgt attcttttgt gtgattttgt gataattcgt ggcaaaaaaa aaacccggca
    32701 tcgccgggtt taaaataata taagtggccg attacacggc gatgagtttg cgggaaggtt
    32761 tcttaacgag attcttcgcg ggattaccga ctactgtggc cccatcgggt acatctttta
    32821 ccacaacggt accggcaccg attgtaacat tgtcgccgat gatgattccc tggcggataa
    32881 ccgagttggc cccgataaat gtattgcggc ctacttttac attaccgcaa agcacggcac
    32941 cgggcgcaat atgggtgaaa tcaccgatga tacattcatg atcaatac
//
LOCUS       DAOPMW010000005        30978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1249_length_30978_cov_10.973838, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000005 DAOPMW010000000
VERSION     DAOPMW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 30978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30978
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_30978_cov_10.973838"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLU11_00550"
     CDS             <1..314
                     /locus_tag="PLU11_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57620.1"
                     /translation="PLDTKEGYFFLSAGNVKSTRVYHYRLSIFEKHDERYRSIKTRFI
                     DNWVRSFSNTYEQIKSELIRNLRDLPNPAVYSIETELDFPVEETLLPVAKRRLVRFIS
                     E"
     gene            complement(446..>2497)
                     /locus_tag="PLU11_00555"
     CDS             complement(446..>2497)
                     /locus_tag="PLU11_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN57621.1"
                     /translation="RDAKPKELQKFYKIWYYKSGMDSAVLLADKNTVGMKLGMTISEF
                     GTISFSKTGKRLFFGTAPIQPPKDTSLVDMDLARLDIWHYKDDYLQTQQLYNLQRLLQ
                     TNYLAVYHLDKKELKQLGSEEIPTVYPTNEGDGEQFVGVTDFGKRVEGQWTGRTKKDI
                     YAINVNSGSLQLVKKDLEGLIAPSFISPSGKYIMWYDSKARHYFAWDGSITRNISQKI
                     KFPLWNEEHDTPDDPGPYGVMGWLQGDTVVYVYDKFDTWSLSLTGKSTPVDITQTGRK
                     NNISYRYLRIDQEERFFISGQKLIYRAFNEKDKSAGIFIGELGSYQPLDYIYFSGNYL
                     LSAIQKAKTADGFIYSTETYRSSPNLYYDMGDFIIQRGDERTKQVKSVQLSNINPQQK
                     DYNWGTAEIYKWKAFTGKMEEGVLYKPENFDPTKKYPMILYFYETHSNTLHSYIPPTP
                     TGSRLNISFFVSRGYVVFSPDIRYTVGHPAKDCYNHVVSAAQDLAKQKWIDAKNIGIQ
                     GQSWGGIQVAQLITMTNMFKAAWAGAPVANMTSAYGGIRWESGVNRQFQYEKTQSRIG
                     ATLWEKPNLYIENSPLFHVPKIKTPLVIMHNDADGAVPWYQGIELFTAMRRLGKQVWM
                     LNYNGEAHNLRERRNQKDISIREQQYFDWLLKGEKPPKWITEGVPAVKKGKDWGLELA
                     N"
     assembly_gap    2498..2597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2598..3582)
                     /locus_tag="PLU11_00560"
     CDS             complement(<2598..3582)
                     /locus_tag="PLU11_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN57622.1"
                     /translation="MRKTAFSLIALFVAIFSYSQKKPLDHTVYDGWQRIGERMISNDG
                     KWVVYTVDPQEGDNELVIQSSDAKYKKTIARGYNAVITEDARFVIFKIKPFFKDTREA
                     KIKKKKPEDTPKDSLAIVELGKDSIWKIGKVKTYKTPEKEAGWVAYHLEKAIEKKEPV
                     KKTDSPEKKIADSLTKVIDSLNKVIQSQPQKRRKNKDEDEESTKYEVRGTDAEGDEPG
                     GAGADAGTDLVLRNLSNGQEKIFANVVEYYFSKNGGKLLLEQAKNPKDSLSKPTVSLV
                     ELATGKSTVLSRGGNDFKNFAFTEDGDQVAYAAERDAKPKELQKFYKIWYYK"
     gene            3657..6098
                     /locus_tag="PLU11_00565"
     CDS             3657..6098
                     /locus_tag="PLU11_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57623.1"
                     /translation="MKQIFALFLLILSNLAAFSQRTGSVTGTVTDKNLQTTLRGVSVE
                     IVGTNLATSTDTAGNFRIAGITPKSYTIRFSSISYTSVELFNIVINSGNELNLNVQLE
                     PSVTNLEDVQVRSSRRTAVAASLETPLSVQRLTTEEIRSNPGGNFDISRVIQALPGVS
                     GTAGSVGGFRNDIIIRGGAPNENVYYLDGIEIPVINHFATQGSAGGPTGILNVSFLEE
                     VKLSSSAFDARFDNALSSVFQFRQRTGNPNKVQGNVRLSATELAGTLEGPLSKKTTFL
                     ASARRSYLQLLFKAIDLPIRPNYWDFQYKVTHRINKKTTLTFLGVGAIDDFTFAAPKE
                     ATPEKLYIIDATAPIHQWNYTVGASLRKQIKNGFWNLSISRNMFDNKIEKFDDNDEGN
                     ESKRRLGILSQEIENKLRFDVNKTVNGWKIAYGMLAQYVKSNNDNYKRLRKEILDQNG
                     TVVQPALVLDYTSDIAFMRYGLFSQVSRRYFDNRLGLSAGLRSDMNGFTNEGMNPSRT
                     LSPRISASYVLADKWTLNASVGLYYKLAPYTVLSFRDNNGNLLNKEADYTRSTHYTIG
                     TEYLPSNSTRFTLEAFYKKYNQVPVSVRDGISLANLGGDFGVVGNEEVVTTGKGTTYG
                     FEFFAQQKLTKRFFGTFSYTFFVSRYSGLNGKEIASAWDNRHLLSFIWGYKFNRNWEL
                     GLKFRYQGGAPYTPFDLTTSQLNYLTFGTGILDYNQLNSQRLEAFHASDIRIDKKWNY
                     RKTTLNLFLDITNWYKAINPSFPQYTFRRTADNTAFLTTDGQPIRQDGSNGIPVFLDN
                     SDASVIPTIGFIVEF"
     gene            complement(6082..6984)
                     /locus_tag="PLU11_00570"
     CDS             complement(6082..6984)
                     /locus_tag="PLU11_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN57624.1"
                     /translation="MKKAFLQLHIAVFLAGFTGILGLLITLNEGLIVWYRLLITALTM
                     WILFALLKKLQVISWADRLKIMGVGFIAAMHWVTFYGAIKYANVSVALVCFSAISFFT
                     ALFEPLILRKKINWTELLLGLVTLSGIYIIFHFDTQFKTGIMIGLVSAVLAALFPIFN
                     REFLKRVNVETLLSWQQTGGFLTLSFLLPFYLQKFPAGHFIPTWSDFGWLLVLAWFCS
                     VLAFQLSGNALKRLSAFTVNLTYNLEPVYGILLAFVVYQENKYLSHWFYWGFAIIAAA
                     LITHIFILIRVERKLTQAEIKTPQ"
     gene            complement(7024..7815)
                     /locus_tag="PLU11_00575"
     CDS             complement(7024..7815)
                     /locus_tag="PLU11_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPN57625.1"
                     /translation="MEKLVIIPTFNEKENITNILHAVFNLNEGFHVLVIDDGSPDGTA
                     QIVKDLQVKFPGQLFIEERRGKLGLGTAYIHGFKWGIARDYAFIFEMDADFSHNPNDL
                     PRLYNACKNEGADVAIGSRYIKGGGVVNWPANRIALSKGGGLYTRLITWMPVMDPTAG
                     FVCYKKEVLETMSLDKIQFVGYAFQIEMKFAAWKLGFKIKEVPIIFQDRTFGTSKMNK
                     GIIKEGVIGVLKLRWNSLFKNYRKRLKNTDTEAPASSKVLVNSEQ"
     gene            complement(7891..8658)
                     /locus_tag="PLU11_00580"
     CDS             complement(7891..8658)
                     /locus_tag="PLU11_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPN57626.1"
                     /translation="MLQLKKPLAFIDLETTGINLGTDRIVEIAIVKILTDGTRSVKRK
                     LINPGIPIPKASSDIHGITDEMVKDAPLFKQVAQELKQMLDGCDFAGYNSNRFDIPLL
                     MEEFLRAQVDFDMKNRKLLDVQNIFHKMEPRTLGAAYRFYCNKTLDGAHSAEVDASAT
                     HEILEAQIEKYAELGNTLDSILKVIGEDQVVDFARRFVMENGIEVFNFGKYKGRPVAD
                     VLKTEPQYYDWMMKGDFPQHTKQKLTEIYTRTLLKKG"
     gene            8889..9011
                     /locus_tag="PLU11_00585"
     CDS             8889..9011
                     /locus_tag="PLU11_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57627.1"
                     /translation="MKNRSLIALLLLTLVVVVSSCASQKYGCPGNPQANYKFRG"
     gene            9023..9358
                     /gene="ytxJ"
                     /locus_tag="PLU11_00590"
     CDS             9023..9358
                     /gene="ytxJ"
                     /locus_tag="PLU11_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HPN57628.1"
                     /translation="MNWKDLLTAQQLDEIIGLSHQQPQVIFKHSTRCSISSTAWNRLK
                     NTEKLKGADFYYLDLLAHRPLSGQIAVALDVEHESPQVLLIKNGKCVYDESHLGITPD
                     ELQDQILNA"
     gene            9608..10468
                     /gene="hisG"
                     /locus_tag="PLU11_00595"
     CDS             9608..10468
                     /gene="hisG"
                     /locus_tag="PLU11_00595"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPN57629.1"
                     /translation="MSETLKIAVQKSGRLYEGSMKLLKECGIEVSNGNNQLRVKAANF
                     PVEIFFLRDDDIPEYVQDAVADIGFVGENVVREKNKQVDPLEKLGFGKCRLSLAIPKN
                     GTLKSVADLDGKKIATSYPVILRDYLREKNINASIHEISGSVEIAPRIGLADAICDLV
                     SSGSTLFTNELMELEVLLQSESVLISNKKLTAEKKIILESLLFRIRSVKKARNNKYVL
                     LNAPNDKLEKICSLLPGMRSPTILPLAESGWSSVHSVISEGDFWDIIEQLKANGAQGI
                     LIIPIEKMIL"
     gene            10468..11757
                     /gene="hisD"
                     /locus_tag="PLU11_00600"
     CDS             10468..11757
                     /gene="hisD"
                     /locus_tag="PLU11_00600"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPN57630.1"
                     /translation="MRQYNYPSPDQWEELIKRPVTDNSALEKTVRRILRKVKENGDRA
                     VRKFTSEFDGVKLKKLQVSEKEIKTAAALLPDELKAAIRLAKENIERFHQSQREAITV
                     TETMPGIRCWRQSLPIEKTGLYIPGGTAPLFSTVLMLAVPASLAGCKEIILCTPPGKD
                     GAVHPAILFTAALCGVTQVFKAGGVQAIGAMAYGTESIPRVFKLFGPGNQYVTLAKQL
                     VQQDRVAIDMPAGPSEVLVIADASAHPAFVASDLLSQAEHGTDSQVILLTPSAALAGE
                     VEKEVEKQLNQLPRKAIAEKALANSRTILFNNMQEAVAFSNYYAPEHLILSCDDAESI
                     AQQVTAAGSVFIGHYSPESAGDYASGTNHTLPTNGYAAMYSGVSLDSFMKKITFQQLS
                     QEGLQRIGDAIMTMAAAEGLDAHKNAIAIRLNTQHGI"
     gene            11747..12799
                     /gene="hisC"
                     /locus_tag="PLU11_00605"
     CDS             11747..12799
                     /gene="hisC"
                     /locus_tag="PLU11_00605"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPN57631.1"
                     /translation="MEFNLDTLVRENIKKMTPYSSARHEFSGAATVFLDANENAFGSP
                     LPVNYNRYPDPLQWPLKEKISQIKGVPPSNIFLGNGSDEAIDLLFRIFCEPGQDNVII
                     FPPTYGMYEVCAEINNVAVKKVPLTRDYQLDLEATEAAVDANTKLIFVCSPNNPTGNS
                     IERGAIEVLLNNFAGLVVIDEAYINYARQKTFITELTEYPNLVILQTLSKAWGLAGLR
                     LGMAFAGQPVIDYMNQVKYPYNINTATQQLALEALSNISSVNNWTKTTVVQKDNLSEA
                     LLALPVTEKVYPSDANFILVKMTDARRIYEYLAGRGIIVRDRSKVMLCDDCLRITIGT
                     PEENNQLLEALKKYMA"
     gene            12796..13935
                     /gene="hisB"
                     /locus_tag="PLU11_00610"
     CDS             12796..13935
                     /gene="hisB"
                     /locus_tag="PLU11_00610"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HPN57632.1"
                     /translation="MRKILFIDRDGTLIQEAPPTYQLDSFSKLAFYPDMFTWMKKIAN
                     ELDFELIMVTNQDGLGTSSFPEETFWPVHEFVMTALENESIFFSNVHIDRTFPADHSP
                     TRKPATGMLTQYLNNPDYDIPGSFVIGDRITDVQLAKNLGCKAIWLNNDASLGAAEVN
                     NDMTTLRDVIGLETISWKDIWEYLRLGQRELVHERNTNETKIRIALNLDGQGKTNIRT
                     GLHFFDHMLDQLGRHSGIDLAITVDGDLHIDEHHTIEDTGIALGEAISLALGNKKGVA
                     RYGFTLPMDDALAQVALDFGGRSWLVWTAEFRREKIGDMPTEMFYHFFKSFSDAAKCN
                     LNIKVEGENEHHKIESIFKALAKTMKMALKRDPENMQLPSTKGVL"
     gene            13935..14528
                     /gene="hisH"
                     /locus_tag="PLU11_00615"
     CDS             13935..14528
                     /gene="hisH"
                     /locus_tag="PLU11_00615"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPN57633.1"
                     /translation="MKTVIIKYNAGNIQSVLVALERIGVQAVVTDDPEEILAAEKVIF
                     PGVGEASTAMQYLKERELDKLIVSLKQPVLGICLGMQLLCAYSEENDTPCLGVFEEVP
                     VRKFRNEKKAFKVPQTGWNSIQQLKGPLFSQVKENEYCYFVHSYYAILNAFTTGTTEY
                     IHPYSSALQRNNFYGVQFHPEKSAKAGEQILKNFLTL"
     gene            14532..15248
                     /gene="hisA"
                     /locus_tag="PLU11_00620"
     CDS             14532..15248
                     /gene="hisA"
                     /locus_tag="PLU11_00620"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPN57634.1"
                     /translation="MTIIPAIDIIDGKCVRLTKGDYEQKKIYNEDPLEVALQFEGAGL
                     KRLHLVDLDGAKAGEVKNWKVLEKIAGKTKLAIDFSGGISTQKNVEITFNSGAAFAAV
                     GSIAVKDEFTFSGWLLAFGAEKFIIGADTKEGKVVIRGWTESTTLTVAELIGRYKTKG
                     VKQFFCTDVEKDGLLQGTAIELYKKILNQHPSIDLIASGGITEVAELDELRQAGCDGA
                     IIGKAIYENRITLADLKRFL"
     gene            15250..16005
                     /gene="hisF"
                     /locus_tag="PLU11_00625"
     CDS             15250..16005
                     /gene="hisF"
                     /locus_tag="PLU11_00625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPN57635.1"
                     /translation="MLTKRIIPCLDIKDGRTVKGTNFLELRDAGDPVELAMRYCEEGA
                     DELVFLDITATTEKRKTLAAMVREIATHINIPFTVGGGINTVEDVRVLLDNGADKISV
                     NTSAVKRPELINELAMQFGSQCVVVAIDAKLINNTWMVVTHGGRTPTLLKALDWAREA
                     AARGAGEILLTSMENDGTKNGFALGITKTISEAVAIPVIASGGAGTMEHFSDVFKKAN
                     ADAALAASIFHFREIPVQELKRYLQERQIPVRL"
     gene            16002..16172
                     /locus_tag="PLU11_00630"
     CDS             16002..16172
                     /locus_tag="PLU11_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57636.1"
                     /translation="MILILFISLCIAWYFYQYFENKRIDRLKEQQEKRKDAYEQLLKI
                     ISEKEDNKHTNT"
     gene            16186..16776
                     /gene="hisIE"
                     /locus_tag="PLU11_00635"
     CDS             16186..16776
                     /gene="hisIE"
                     /locus_tag="PLU11_00635"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPN57637.1"
                     /translation="MQPDFSKYNDGLIPVIIQDAGTLQVLMLGFMNEAAFLQSQQEGK
                     VTFYSRSKQRLWTKGETSGNFLHIQEIKADCDNDTLLIRVLPAGPVCHTGTDTCFNEK
                     NPPFSLQQLERIIIDRKNNPDEKSYTASLFAKGINKVAQKVGEEAVELVIESKDDNQE
                     LFLGEAADLLFHYLVLLQHKGFTLADVVHTLEKRHK"
     gene            complement(16783..18978)
                     /locus_tag="PLU11_00640"
     CDS             complement(16783..18978)
                     /locus_tag="PLU11_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57638.1"
                     /translation="MRIKWSDLKKVIGEVQLSREKYISLIREDGTILSANSRMTRSLH
                     LSSCKDNVLNLFDLLHPVNRKSFAERLSESDKEGTVSCVEAYLKNGLYHPMKWHITPM
                     PANGESRAYLCQGYKLLDEDRTRAFYRLGEYNYQLIFDSLDAGILFQDARGEIIAANQ
                     KTAEILNISLEDLYNLTRIAIQWNKSWDIRNEQDQPVLFADTSFIKALETRKRHTEVL
                     VIRLKNGEQRWLHLSSQPLFRDHSSIPFAVVSSITDLTLEKKLIRELKERKAVLRSFM
                     KQTPNLAWVVDDQSNLLFASRHFYEFFGIDEINAPNKSILSLVPPAVAEALLGIHLRV
                     LETGISEDHIQRIKWADGSDYVFHIDIFPIEGISGRKILGGHAVNLTEKFNAEKRLRE
                     TNDRLLLLSRATSDAIWEWDMQTGQIFRNEALMDLIGYHTDIPKGLSWWLRRIHPEDR
                     NRVGDAVKQSTEEGKQSWEGEYRFKCADGEYKHMRDKGYIVYENGLPVKMIGSIQDIS
                     NFKELEKMLIEERLKRHKDVSELVIQAQEKERTQIGHELHDNVNQILSSAKLFVEMIQ
                     ADAADQRLYQSKGLDYISLAIEEIRKLSKELVAPRLKENNLISCISQLLEDARMASKI
                     DIHFIQDLSADLLSEGKKVTLFRILQEQLKNILKHSSATRVEVLLESDDQWVRMLVKD
                     NGIGFEANHTFRGIGLSNIYERVRFYDGAADIKTAAGEGCQLIVSIPIL"
     gene            complement(18968..19444)
                     /locus_tag="PLU11_00645"
     CDS             complement(18968..19444)
                     /locus_tag="PLU11_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN57639.1"
                     /translation="MYYSPLMGLFFYLYRNTNLIPVLKNTLNILIVDDNLNFIKRMIS
                     ILDDVKYPKKISIASDFNGAMQEMAAGGQDILLLDINMPGKSGIELLRYIREKNPGTE
                     VIMLTNHAEAYYREQCRELGAQHFLDKSNDFLQVPEIVNAYCRKNIETSQSDQYAD"
     gene            complement(19484..20116)
                     /locus_tag="PLU11_00650"
     CDS             complement(19484..20116)
                     /locus_tag="PLU11_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57640.1"
                     /translation="MIRILIADDHAIVRRGMKQLLLEQYPFARIGEASNVEELIHEIV
                     LHKDWDVVVCDMNMPGRSGLDAIMQIKEIAPGLPVLIMSMYPEDQYALRVLKSGAAGY
                     LTKETIHDDIVRAIETVIKGKRYITLSLAEKLAESVNHETEKPLHEVLSDREFDVFKL
                     LASGKTITEIASQLLLSSTTVSTYRSRIMEKMKMKSNAELARYAIESRLI"
     gene            complement(20141..25660)
                     /locus_tag="PLU11_00655"
     CDS             complement(20141..25660)
                     /locus_tag="PLU11_00655"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57641.1"
                     /translation="MNRIYTRQSITIAVVITFVLVSFFAWYSYVNMKRARYETLQVNK
                     TLQSLKVLENLMDDVQDIETANRGFLISANRDFLTPYDAAISNLSTDTTAILSLDGIS
                     ESRKDSLKILVRLTNKKLKIAAQTEALVSKNKVDSALLLVRSGTGMEIMDSIRNLVLA
                     LETTDRKVLNSSNSSREIAATNTARLFVILSICFILLTSLIFFIIHRDLKRKEQYEAR
                     IAYLAELTERTSDAIISIDPQQIVISWNKGAHNIYGYSSEEAIGRKISELIQSSALEN
                     GDQDIQREIAIKGGIEFESNDFTKDGKSIYCHISATALRDVHGKLTGFVSVVRDITQR
                     KLAEKLIYEFNHELAAKVKEKTEDIRKGEEKLRQVLDSAAGEFYVIDLDYRVILMSRM
                     AEVNLESVWKHVIRSGDRIIDFIPENRKEVVLESYNRAFGGETIEYETRVESGQQLRW
                     VSIQLTPVRNEFNEITGAFVGTRDISARKNAEAGLQESESRYRTLVEQATETIVLVDE
                     RGHFIQVNEAGVRLLGYSREEVMLMSLMDILVLEPGDPPLRFEQLRTGASILSNRKVR
                     RKDGVVIELELSTKMLSNGHFIGIGRDISERMQVQKALTESENRFRAIFNTQLNMVTL
                     LDKDGRILETNKTALAITGYRNEEVIGRYFWDIRLWQNEEERTARIEKVKEAITRAAA
                     GELIRYEGQISLKEGEVEVVDLSIKPIPGVDGKPMLLIMEGRLITEIKKAENEVKESE
                     AKFRSFFEYSMDGILLSSPDGAILSANPAACQMFGMSEAEIVALGRAGLIDQEDERIH
                     GLLDLRERMGYLTGELSFVRKDGTKFPGEFSSAIFKDAFGRERTSLIIRDVTERKRIE
                     EEIRQSNKRFEMISRTTNDAIWEWNILTGRKWANENHQQLYGLTIEDPVPEEYVWRSR
                     IHPDDRALIVSRQESTLASEKNVFISEYRFLNGENEYVYLFDRCYIERDDAGKPLRMT
                     GSMMDISDRKRSEEQLMESEQRLRLSMEAAKQGLYDLNIKTGEAIVNEQYALMLEYDP
                     RDFNENNELWLERMHPDDLPAAQKAFADYVSGITGEFRTEFRQRTQSGKWKWILSIGK
                     ILEYDDQGNALRMLGTHTDINSLKNAEEELIRTQKRFQNLVENISGVYWVNDLETQHT
                     LYISPSYETVWGRKCEDLYRNPADFVKSVHPDDLKKLVEAYHRIAEKKQFNLNYRIIR
                     PQGDIRWIAVKINVVSGADGRRMEYGYAEDITEQRKAETDLLESNARFQIVSRATSDL
                     VWDWNLLAGELWWNDNYYSSLGYRKTSELVDENEWYDRIHPDERERVRLNANKTFKGK
                     SSVWRDEYRYRKADGSYLHFLDRGFIMRDPGGQAIRMIGSMIDMTPIYTVQRKIEESE
                     MRLRTILDTDPECIKLMDEDACLLDINRAGLQMIEAASIEMVKGKSVLPLIDEGQQQQ
                     AIRLIKDAFKGKSGTLEFQMITLKGMKRWCELSVVPFLNTDKKIISALGVTKDITERK
                     QAETAVRVSEEKYRTLVEQAADSIALYDSDGILLDTNTSASRLLGYSREDFSQMKLSD
                     VLLPEDIVVNPVQFDVLSSGISTVKIRRMRRKDGTVVITEVRSQQLPDGRFLSVIRDM
                     TERIKAEEELKSSYNAVRSLTAHLQNIREEERTNIAREIHDELGQQLTVLKMDVAWLN
                     KRFQGTDDKVNQRLKDLLIMLDETVQSVRRISSQLRPSLLDDLGLTAAMEWQLGEFEK
                     RAGIKTSFEAPQDEIPVPEAAKTALFRIFQESLTNVVRHSEAKKLAVSVKEADNCLVM
                     TIADNGKGFDPMKVAEKKTLGILGMKERTEMIGGTYIINSLPGQGTSVIVSVPVI"
     gene            complement(25707..26342)
                     /locus_tag="PLU11_00660"
     CDS             complement(25707..26342)
                     /locus_tag="PLU11_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57642.1"
                     /translation="MGKITILLVDDHKLIRDSWSFILNSDSRFQVIGETSNADEAVEI
                     ARTKKPDIVLMDINMTPVNGFEATKLVRKFSPGSKIIGISMHSMPAYARRMLQIGAMG
                     YVTKNSSKDELINSIVEVYGGKKYICEEVKNILAQQELEDEGGTPDMNVLSRRELDIV
                     QLIKEGLSSKEIAIRLDISLKTVEVHRYNILKKLSLKNTAALVNFINVHGL"
     gene            complement(26561..27742)
                     /gene="uxuA"
                     /locus_tag="PLU11_00665"
     CDS             complement(26561..27742)
                     /gene="uxuA"
                     /locus_tag="PLU11_00665"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPN57643.1"
                     /translation="MKRMEQTMRWFGPNDPVSLADIRQAGCTGVVTALHHIPVGDVWT
                     MEEILKRKALIEEAGMTWTVIESLPVSEDIKKQQDNYVKHIENYKQSLRHVAACGLKV
                     VTYNFMPVLDWMRTDIAWLMPDGSRALYFERAAFVAFDLFMLKRPGAEKDYTAAEIRK
                     AQFRFDRMTDEEEERLFHNALLGLPGSEDRFTEEQILTALETYKGIDAARLKQHLFYF
                     LREVIPVAETLGLQMAIHPDDPPYPVLGLPRVVSTEKDAAELLAAAPSTANGLCFCTG
                     SFGARADNDILQMLKRFGDRVHFLHLRNTKRDAEGNFYEADHLAGDTDMYEIIKEVLR
                     IQQRRDCSIPMRPDHGHQMLDDLQKETYPGYSAIGRLRGLAELRGLEYGIARKMTYDE
                     G"
     gene            complement(27832..29160)
                     /gene="xylA"
                     /locus_tag="PLU11_00670"
     CDS             complement(27832..29160)
                     /gene="xylA"
                     /locus_tag="PLU11_00670"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPN57644.1"
                     /translation="MGVVTGNKEYFKGIGQIKYEGLGSDNPLAFRWYDENKIVAGKPM
                     KEHLRFACAYWHSFCGSGADPFGEPTHLFPWSEKTDPVERAKDKADAAFEFITKLGLP
                     YYCFHDVDAVDYSNDINENDRRLQAITAYFSEKQKASGVKLLWGTANLFSNRRYMNGA
                     ATNPDFHVLTHAAAQVKAALDTTIALGGENYVFWGGREGYMSLLNTNMKREKEHLARF
                     LHTAKDYARKQGFNGKFFIEPKPCEPSKHQYDYDCETVIGFLRQYDLLNDFSLNIEVN
                     HATLAGHTFTHELQVAADAGLLGSMDANRGDYQNGWDTDQFPNDINELTEAMLVILEA
                     GGFKGGGINFDAKIRRNSTDPEDLFYAHIGGMDVFARALITADNILQQSDYKKVRADR
                     YSSFDNGKGKDFETGKLSLEELRSLALENGEPSVISGKQEFLENLINRYI"
     gene            complement(29187..30665)
                     /locus_tag="PLU11_00675"
     CDS             complement(29187..30665)
                     /locus_tag="PLU11_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPN57645.1"
                     /translation="MLLLGIDLGTSSVKVSVIDATSQQTVATAQYPETEAEIISVHPG
                     WAEQSPESWWEQVQLAVLKVHAQRAYNPADIGAIGIAYQMHGLVAVDKDQQVLRHSII
                     WCDSRAVETGDKAFESIGREKCLLHLLNSPGNFTASKLAWVKENEPDVYEKIDKIMLP
                     GDFIAMKLTGTITTSIEALSEGVFWDFKENGLSEDVFNYYGFSRSLIPEIRDVFSEHG
                     QLTPKVAGALSLRVGIPVTYKAGDQPNNALSLNVLEPGEVAATAGTSGVIYGVSDQLA
                     YDHESRINTFAHVNYTREQRRLGVLLCINGTGILNRWVKNISGDLMGYAELNHKAAAV
                     APGSEGVMVFPFGNGAERMLNNRVMNAQFLHIDFNKHGIAHVYRAAQEGIVFAFRYGL
                     DIMRQNGMQPGVIRAGKANMFLSDVFTSAFVNTLNVPVELYQCDGSHGAAIGSGIGAG
                     FYKNPGEAFSNFAPVETIRPAAGHQYDELYAKWLEQLEKNLN"
BASE COUNT         8306 a         7648 c         6453 g         8471 t
ORIGIN      
        1 tacccctgga taccaaggaa ggttattttt tcctgagtgc cggaaatgta aaaagcaccc
       61 gggtctacca ctaccggctt tccatctttg aaaaacatga tgagcgttac cggagcatca
      121 aaacccggtt tatcgacaac tgggtccgca gtttcagcaa cacctacgaa cagatcaaat
      181 cagaactgat ccgcaacctc cgcgatctgc ccaaccccgc ggtgtattcc attgaaacag
      241 aacttgattt tccggtagag gaaacgttgt tacctgtggc gaaacggaga ttggtgcggt
      301 ttattagcga atgattttgg ctaagaggtc aaaagagatg aaaggagaaa aaaagagggc
      361 gtgattaggg gaatgtcttt ataacccttt aaacctctta aacctcttaa acttcttaaa
      421 ccttgcacac acattggcat attggctaat tggctaattc taatccccag tcctttcctt
      481 tcttcacggc cggtacgcct tcggtgatcc atttgggcgg cttctcccct ttcaataacc
      541 agtcaaaata ttgttgttcg cgaatagata tatccttctg gtttctcctt tccctgaggt
      601 tatgagcttc gccattatag ttcagcatcc acacttgttt gcccaggcgg cgcatggcgg
      661 taaagagttc aattccctgg taccagggaa cggcgccatc ggcatcattg tgcatgatca
      721 ccagcggagt tttaatcttc gggacatgaa ataaaggtga attctctata taaagattcg
      781 gcttttccca taaagtggcc ccgatccggc tctgtgtttt ttcatattgg aactgacggt
      841 tcacgccact ttcccagcga ataccgccat aggcactggt catattggcc accggtgccc
      901 cggcccaggc ggctttaaac atattggtca tggtaatcag ttgtgccacc tgtataccac
      961 cccagctctg tccctggata ccaatattct ttgcatcgat ccacttctgt ttagccaggt
     1021 cctgcgccgc actcaccaca tgattataac aatctttggc cggatgcccg actgtatacc
     1081 ggatatccgg agagaaaacc acataaccgc ggcttacaaa aaaggaaata ttcagccggc
     1141 tgccggtggg tgtaggcgga atataactat gcagggtatt ggaatgtgtt tcgtagaaat
     1201 aaagaatcat cgggtatttc tttgtgggat cgaaattttc gggtttgtac aagacccctt
     1261 cttccatttt accggtaaag gctttccatt tgtatatttc ggcggtgccc cagttgtagt
     1321 ctttttgctg ggggttaata ttcgataatt gtactgattt tacctgtttt gtacgctcat
     1381 ctccacgctg aataataaaa tcacccatat cataatataa gttaggtgag gaacgatatg
     1441 tttcggtact gtagataaat ccatctgcag ttttcgcctt ctgaatagca gaaagcaaat
     1501 aatttcctga gaaataaata tagtctaatg gttgatatga ccccagttct ccaataaaaa
     1561 ttccggccga tttatccttt tcattaaaag cacgataaat caatttttgg ccagaaataa
     1621 aaaagcgttc ttcctggtcg attcgcaagt atcggtaact gatattgttt tttcgtccag
     1681 tttgtgttat atcaacagga gttgatttcc ccgtcaaact caggctccat gtatcgaatt
     1741 tatcataaac atatacgact gtatcacctt gaagccatcc catcacccca tacggtcccg
     1801 gatcatccgg cgtgtcatgc tcctcattcc agagcgggaa ctttattttt tgagagatgt
     1861 ttcgggtaat gctgccatcc caggcaaaat aatgacgggc cttactgtca taccacataa
     1921 tatatttacc acttggtgaa atgaatgaag gcgcaatcaa cccctccagg tcttttttaa
     1981 ctaactgaag cgagccactg ttcacattaa tcgcataaat gtcttttttt gtacgcccgg
     2041 tccactggcc ttctaccctt tttccaaagt cagtaacacc cacaaactgc tcaccatccc
     2101 cttcattggt tggatacact gtaggaattt cttctgaccc taattgcttc agctcttttt
     2161 tatccaggtg ataaaccgcc aggtaattag tctgcaataa acgctgcaga ttgtataatt
     2221 gctgggtttg caggtaatca tccttataat gccagatatc cagtctggcc aggtccatgt
     2281 ccaccaggct ggtatcctta ggcggctgaa taggggcagt gccaaagaac aaacgctttc
     2341 cggtcttact gaaactgatg gtcccgaatt cactgatggt catgcccagt ttcatcccca
     2401 ctgtattttt atcagctaat aacacagcac tgtccatccc ggatttataa taccatattt
     2461 tatagaattt ctgcaactcc tttggtttgg catcccgnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnatt tataatacca tattttatag aatttctgca actcctttgg
     2641 tttggcatcc cgctcagccg cataagccac ctgatcgccg tcttctgtaa atgcaaaatt
     2701 cttaaaatca ttgccaccac gactcagtac cgttgacttc cccgtcgcta attcaaccaa
     2761 cgaaacagtg ggtttactca atgaatcttt cgggttcttt gcctgctcca acaacaactt
     2821 acctccgttc ttactgaaat aatattcaac cacattggca aatatctttt cctgcccgtt
     2881 tgaaagattc ctcaacacca ggtcagttcc ggcatccgcc cctgctcctc caggttcatc
     2941 accttccgca tccgtacctc gtacttcgta cttcgtactt tcttcatcct catccttgtt
     3001 ctttcttctt ttttgtggtt gcgactgaat aaccttgttt aacgaatcaa ttactttagt
     3061 aagactatct gctatttttt tctccggtga atctgttttc ttaaccggtt cttttttctc
     3121 tatggctttt tctaagtggt aagcaaccca accggcttct ttctccggtg ttttgtacgt
     3181 ttttactttt ccaattttcc atatactgtc tttgcccagt tccacgatgg ccagtgaatc
     3241 cttcggggta tcctcgggct ttttcttttt tatttttgct tcacgggtat ctttaaaaaa
     3301 aggtttgatc ttaaaaatca caaagcgggc atcctccgtg attacggcat tgtacccacg
     3361 tgcaatggtc tttttgtatt tcgcatccga cgactggatc accagctcat tatccccttc
     3421 ctggggatca acggtataca caacccactt cccgtcatta ctgatcattc gttcgccgat
     3481 ccgttgccag ccgtcgtaaa ctgtatgatc caatggtttt ttctgggaat aactaaaaat
     3541 ggccacaaac agggcgatca gggaaaaagc agtttttctc ataataaata atgatggtca
     3601 acaaattaaa gcaaacaatt caattactta gctgttctat atcagcaatt cctaatatga
     3661 aacaaatttt tgcgcttttc ctgttgattt tgtccaacct ggccgctttt tcacaaagaa
     3721 ccggctcggt gactggtact gtgaccgaca aaaacctgca aaccaccctc cgtggagtga
     3781 gcgtggaaat cgtcggaacc aacctggcta ccagtacgga tacagccgga aatttccgga
     3841 tcgctggcat aacgcctaaa tcatatacga tccgattcag ctctatttcc tatacatcgg
     3901 ttgaactttt caatattgtg atcaattccg gtaatgaact gaacctgaat gtgcaactgg
     3961 aaccttcggt gaccaatctg gaggatgttc aggtacgtag tagccgtcgt actgcagtgg
     4021 ctgcaagcct ggaaacccca ctaagcgtac agcgactgac cactgaagaa atccggagta
     4081 atcctggcgg taacttcgat atctcccggg taattcaggc gctcccgggc gtgagcggaa
     4141 cagcaggaag tgtgggtggt ttccgtaacg acatcatcat acggggtggc gcacctaacg
     4201 aaaatgtcta ttacctcgac gggatagaaa ttccggtgat caatcacttc gccacccagg
     4261 gtagtgctgg tggtcccacc ggtatcctga atgtgagttt cctggaagaa gtaaaactga
     4321 gttcttcggc ctttgatgcc cgctttgata atgccctctc ttctgttttc cagttccggc
     4381 aacgcacggg caacccgaat aaagtacagg gaaatgttcg gctcagcgcc acagaactgg
     4441 caggaacttt ggaagggcca ctctcaaaaa aaacaacctt cctcgcgtca gcccgtcgct
     4501 cctatctcca gttgctcttc aaagccatag acctgcccat tcgtccaaac tattgggatt
     4561 tccagtataa agtaacgcac cggatcaata aaaagaccac ccttacattc ctgggtgttg
     4621 gcgccatcga tgattttacg tttgcggccc ctaaagaagc tacccccgaa aaactctata
     4681 taatcgacgc taccgctcca atccatcaat ggaattatac tgtaggcgcc agcctgcgta
     4741 aacaaatcaa aaacggtttc tggaacctgt ccatcagccg gaatatgttt gacaataaga
     4801 ttgagaaatt tgacgacaac gatgaaggga acgaaagcaa acgccggctg ggtatcttgt
     4861 cacaggaaat cgagaacaaa ctacgctttg acgttaacaa aactgtcaac ggttggaaga
     4921 tcgcttatgg gatgttggca cagtatgtta agagtaataa tgacaactac aaacgcctgc
     4981 gaaaagaaat tctggaccag aatggtaccg tcgttcaacc cgccctggtg ctcgattata
     5041 cgtccgatat cgcattcatg cgttatggac tattctccca ggtaagccgt cgttatttcg
     5101 acaaccggct gggccttagc gccggtctgc gttcagacat gaatggattt accaatgaag
     5161 ggatgaatcc gtcgcgaacg ctctctcccc gtatcagtgc ttcatacgtc ctggcagata
     5221 aatggaccct gaatgcgtcc gtcggtcttt actacaaact ggccccctac accgtcctta
     5281 gtttccggga caataatggc aacctgctga ataaagaggc tgattatacc cgcagtaccc
     5341 attataccat tggcactgaa tacctccctt cgaacagtac ccgatttacc ctggaagcct
     5401 tttacaaaaa gtacaaccag gtaccagtct ctgtcagaga tggaatatca ttggcaaacc
     5461 tcggcgggga ttttggcgtg gtaggaaatg aagaagtcgt gactactgga aaagggacta
     5521 cctacggttt tgaattcttt gcccagcaaa aactgactaa acggttcttc ggcacgttct
     5581 catatacgtt ctttgtcagc cgctatagtg gcctgaatgg aaaagaaatc gccagtgcct
     5641 gggataaccg ccacctcctc tcttttatct gggggtataa atttaaccgc aactgggaac
     5701 tgggacttaa attccgttac cagggcggcg caccttatac gccgtttgat ctgaccactt
     5761 cccaattgaa ttacctgaca ttcggcaccg gcattctgga ctacaaccag ctcaacagtc
     5821 aacggctgga agccttccat gccagcgata tcaggatcga taaaaaatgg aattaccgga
     5881 agaccaccct gaatcttttc ctggacatca ccaattggta taaagcgatc aatccttctt
     5941 tcccgcagta tacgttcagg agaacagcgg acaataccgc atttctgact acagatggac
     6001 aacccatccg gcaggatggt tccaatggta tccccgtttt ccttgataac agcgacgcga
     6061 gtgtgatccc gacaatcggg tttattgtgg agttttaatc tccgcctgtg tcagtttacg
     6121 ttcaacccgt atgagaataa atatatgggt gatgagggcg gctgctatga tggcaaaccc
     6181 ccagtagaac caatgactga ggtatttatt ctcctgatat actacaaaag ccagcaatat
     6241 tccataaacc ggttcaaggt tataagtcag attgactgta aaggccgaca atctttttaa
     6301 agcattaccg gatagctgaa aggccagtac agaacaaaac caggccagta cgagcaacca
     6361 gccgaaatca ctccaggtgg gtataaaatg accggccgga aatttttgca gataaaaagg
     6421 caacaggaaa gagagcgtaa gaaagccgcc agtctgttgc cagctcagta aggtctctac
     6481 attcacccgt tttaaaaact cccggttgaa aatcggaaac agggcagcca atacagcgga
     6541 aaccaaaccg atcattatcc ctgtcttgaa ttgagtatca aaatgaaaaa tgatatagat
     6601 cccgcttagg gtaaccagac cgagcaataa ttcggtccag tttatttttt tccggagaat
     6661 aagcggttca aataaagcgg taaaaaaact gatggcggag aaacagacca gggccacgga
     6721 tacatttgca tatttaatgg ccccgtagaa agttacccag tgcatagctg caataaagcc
     6781 cacacccata attttcaacc ggtcggccca tgatatcacc tgcagttttt tcaacagggc
     6841 aaacaggatc cacatagtca gcgcagtgat cagcagacgg taccagacaa tcaatccttc
     6901 gttgagggta atcagcagtc cgagaatgcc tgtaaatccc gccagaaaga cagcaatatg
     6961 caattgcagg aaggctttct tcattgcctg taaattagga aaaaggaatg aatgaatggc
     7021 tggttattgt tcggaattga ccagtacttt agaagaagca ggtgcttcgg tatccgtatt
     7081 tttaagccgt ttccggtaat ttttgaaaag gctgttccag cgtagtttga gtaccccgat
     7141 caccccttct tttatgatac ccttattcat ttttgaagta ccgaatgtgc ggtcctgaaa
     7201 gatgatcggc acttctttta ttttaaaacc gagtttccag gcggcaaatt tcatttcaat
     7261 ctggaaggca taacccacaa actgaatttt atccagactc atggtttcga gcacttcttt
     7321 tttatagcag acaaatcctg ccgtgggatc catcaccggc atccaggtaa tcagccgggt
     7381 atataaaccg cctcccttgg aaagagcgat acggttagcc ggccaattga cgacccctcc
     7441 tccttttata taacgggaac cgatagccac atccgcccct tcatttttac aggcattata
     7501 caaccgggga aggtcattgg gattgtggga gaaatcggca tccatttcaa aaataaatgc
     7561 ataatcccgc gcaattcccc atttgaatcc atggatataa gccgtaccca gccccagttt
     7621 accccggcgt tcttcaataa ataactgtcc cggaaatttc acctgaaggt cttttacaat
     7681 ttgtgcagtg ccatccggcg aaccgtcatc aatgaccagc acatgaaagc cttcgttgag
     7741 attaaaaaca gcatggagga tattcgtaat attctccttt tcgttgaagg tcggtatgat
     7801 gactagtttt tccacgcccg gctatataaa atttactaac aaaagtaaga taaacagtaa
     7861 tcaagccctg ttaaaattca ggtaaagggt ttaccctttt tttagcaggg ttcgggtata
     7921 aatctctgtc agcttctgtt tggtatgctg gggaaagtct cccttcatca tccaatcata
     7981 atactggggc tccgttttca gtacatctgc caccgggcgc cccttatatt tcccgaaatt
     8041 aaaaacttcg atcccgttct ccatcacgaa acggcgggca aaatccacca cctgatcctc
     8101 tccgatcact ttcagtatcg aatcaagggt atttcccagc tccgcatatt tttcaatctg
     8161 tgcctccaga atctcatgcg tggcactggc atccacttcc gcactgtgtg ctccatccag
     8221 cgttttgtta caataaaaac ggtaagcggc tccaagagtc cggggctcca ttttatgaaa
     8281 aatattctgt acatccagca gtttgcggtt tttcatatca aaatccacct gggcacggag
     8341 aaactcctcc atcaacaagg ggatatcaaa ccggtttgaa ttgtaaccgg caaaatcgca
     8401 cccatccagc atctgtttca gctcctgggc cacttgttta aaaagcgggg catctttgac
     8461 catctcatcc gtgatcccat ggatatcact ggaagccttg ggaatgggaa ttccgggatt
     8521 gataagttta cgcttcacac tacgggttcc atcggtcagg attttcacta tggcaatttc
     8581 aactatgcgg tctgttccca ggttaatacc cgtggtttcc aggtcaataa aagccaatgg
     8641 ttttttcagt tgcaacatca gggttgtgtt tcttattaaa tgaggcggta aagttaactg
     8701 tttgtaaaac tccgcaaaat gaaattattc ggaaaaataa gggttaacct aagaaaacca
     8761 gtcatttaac tacccaaaat caggtaaaag actgataaaa tacactttga aaagccggga
     8821 agggacttat ctttgcagta acaaaataat ccgtttatta aatcgtttat ccaccaaata
     8881 tcccttttat gaaaaataga tccctgattg cccttttatt actgaccctg gtagttgtcg
     8941 tttcatcctg tgcttcacag aaatatggct gtcccggtaa tccccaggct aattataaat
     9001 tcagaggtta gtcccaatca atatgaattg gaaagatttg cttaccgccc aacaactgga
     9061 cgaaatcatc ggtttgtccc accagcaacc ccaggtaatt ttcaaacaca gcaccagatg
     9121 cagtatcagc agtacagcct ggaaccggct taaaaacact gaaaaactga aaggagccga
     9181 cttttactac ctggacctgc tcgctcatcg tccgctttca ggtcagatag ccgttgccct
     9241 ggatgtggaa cacgaatcgc cccaggtatt actgataaaa aatgggaaat gtgtctatga
     9301 tgaaagccat cttggaatca ctccggatga actacaggac cagattttaa atgcctgagc
     9361 ttaagggttt ctaaaaacaa gtaatgaatg ttcgttttta tgatcccgca gactcaatac
     9421 aaacagaaaa tcatctgccg ttaagctatc aattaaaata atccttattt ttgcaccgca
     9481 atgagaaatt acacacataa ccatcatcat cactttccct cgcaagggta ggccatgttg
     9541 ttattatgtg ttaacatagt tcggggctta cttcaggtag gccctttttt tattttaaac
     9601 tgtttatatg agtgagacat taaaaatagc cgtacagaaa tcagggcggt tatacgaagg
     9661 atcaatgaaa ttgctgaaag aatgcgggat agaagtcagc aacggaaaca accagttaag
     9721 ggtaaaagcc gccaatttcc ccgtagagat tttcttcctt cgtgatgatg atattcctga
     9781 atatgtgcag gatgccgtgg cagatatcgg attcgtcggt gaaaatgtgg tccgggaaaa
     9841 aaacaaacag gtagatccgt tggaaaaact gggttttgga aaatgcagat tatcgctggc
     9901 cattccaaaa aatggcacat tgaaatcggt ggctgatctg gatggcaaaa aaatagccac
     9961 cagttatccg gtcattctta gggattattt aagggagaaa aacattaatg cctccataca
    10021 tgagatcagt ggctctgtgg aaattgcacc acgtatcgga ctggcagatg caatttgtga
    10081 tctcgtgagt tccggctcca cactcttcac caatgagctg atggaactgg aagttttact
    10141 gcaatctgag tcagtgctta tcagcaataa aaagctgaca gccgaaaaga aaatcatact
    10201 ggaaagcctg cttttccgca tccgttcggt caaaaaagca aggaacaata aatacgtctt
    10261 gctgaatgcc ccgaatgata aactagaaaa gatctgcagc ctgttgcctg gtatgcggag
    10321 cccgaccatt ctcccgctgg cagaatccgg ttggagttca gtacactcgg tcatcagtga
    10381 aggtgacttc tgggatatta ttgaacaatt aaaagctaac ggggctcaag gcatcctgat
    10441 tattccgatt gaaaagatga tattataatg cgacaataca actatccctc ccctgatcaa
    10501 tgggaagaac tcataaaacg acccgtaacg gacaattccg cgttggaaaa gacggtacgc
    10561 aggatcctcc ggaaagttaa agaaaacgga gaccgtgctg tacggaaatt cacgagtgaa
    10621 tttgatggag tgaaactgaa gaagctgcag gtctccgaaa aagaaattaa aaccgccgcg
    10681 gcactcctcc cggacgagct gaaagccgcc attcgcctgg caaaagaaaa cattgaacgg
    10741 tttcaccaat cacaacgtga agcaataacc gtaactgaaa caatgcccgg tatacgatgc
    10801 tggcggcagt cattgcccat tgaaaaaaca ggcctttata ttccgggggg caccgcccct
    10861 cttttctcga ccgtactgat gctggcggta ccggccagtt tagcgggttg caaagaaatc
    10921 attctctgca cgccccctgg aaaagacggc gctgtacatc cggcgatatt atttacagcc
    10981 gccctatgcg gggttacaca ggttttcaag gcagggggcg ttcaggcaat cggggccatg
    11041 gcctatggaa cagagagcat tccccgggtt tttaaactat ttggcccggg taatcagtat
    11101 gtcaccctgg ccaaacaact tgtacaacag gatcgtgttg ctattgatat gccggctggc
    11161 ccttctgaag tactggtcat tgccgatgca agtgcccacc ccgcttttgt cgcgtcggat
    11221 ttattatccc aggccgaaca tggtaccgat tcacaggtaa tcctgctgac cccctctgcc
    11281 gcgttggccg gagaggtgga aaaagaagtc gaaaagcaat tgaaccagct tccacgtaaa
    11341 gccattgccg aaaaggcact ggccaacagc agaacaatcc ttttcaacaa catgcaggaa
    11401 gccgtggcat tttccaatta ctatgcaccg gaacacctta tcctgtcctg tgacgatgcg
    11461 gaatccatcg cacaacaggt aacagccgcc ggttccgttt ttatcggtca ctattcaccg
    11521 gaaagtgccg gagattatgc cagtggcacc aaccataccc tgcccaccaa cggctatgcc
    11581 gccatgtaca gcggggtatc attggacagc tttatgaaaa aaattacctt tcagcaactg
    11641 agtcaggaag gtctgcagcg cataggcgat gccattatga ctatggccgc cgctgaagga
    11701 ctggatgctc acaaaaatgc catcgccatc cgcttaaata cacaacatgg aatttaatct
    11761 cgatacactg gtcagggaga atataaaaaa gatgaccccc tactcctccg cccgtcacga
    11821 attcagcggg gcagccacgg tgttcctgga tgccaacgaa aatgcattcg gatccccact
    11881 gccagttaac tataaccgct acccggatcc gctgcaatgg ccactgaagg aaaaaatcag
    11941 tcagataaaa ggcgtgcccc catccaatat tttcctgggc aatggcagtg atgaagccat
    12001 cgacctgctc ttccggattt tttgcgaacc ggggcaggat aatgtcatca tcttcccgcc
    12061 gacttatggc atgtatgaag tatgtgccga aatcaataac gtggcggtaa aaaaagttcc
    12121 gctgacaagg gactaccaac tggatctgga ggccacggaa gcggctgttg atgcaaacac
    12181 taaactgatt tttgtctgtt cacccaataa cccgaccggc aacagtattg aacgcggtgc
    12241 catcgaagta ctgctgaata attttgccgg actggtagta atcgatgaag cctatattaa
    12301 ttacgcccgc cagaaaacgt ttattactga actaacggag taccctaatc tcgtgattct
    12361 acaaaccctg tcaaaagcct ggggcttggc cggactgcgg ctgggcatgg cctttgccgg
    12421 gcagccagta attgattata tgaaccaggt caagtaccct tataatataa ataccgccac
    12481 tcaacagctg gcactggaag cattgagtaa tatcagtagc gttaataact ggacaaaaac
    12541 aaccgtagta cagaaagaca acctgagtga agccttgctc gctttgcccg ttacagaaaa
    12601 agtatatccc tctgatgcca attttatatt agtaaaaatg accgatgccc gccggatcta
    12661 tgaatatctt gctggccgtg ggattattgt ccgggatcgt tctaaagtta tgttgtgtga
    12721 cgattgcctg cgcatcacta ttggcacccc cgaggaaaac aaccaattac tggaagccct
    12781 taaaaaatat atggcatgag aaagatttta ttcatcgacc gggatggcac cctgatccag
    12841 gaagcccctc ccacctacca gctggactct ttttccaaac tggcctttta cccggacatg
    12901 ttcacctgga tgaagaaaat tgctaatgaa ctggactttg aactgataat ggtgaccaac
    12961 caggatggtc ttggcacctc ctcttttccg gaagagacat tctggccggt gcatgaattt
    13021 gtcatgacgg cgctggaaaa tgaatcgata tttttcagca atgtgcatat agaccgtact
    13081 ttccctgcag atcactcccc tacgcgtaaa cctgctaccg gaatgctgac ccaatacctg
    13141 aacaatccgg actatgatat acccggatct tttgtaattg gcgaccggat caccgatgtg
    13201 cagctcgcaa aaaatctggg ttgcaaagcg atctggctga acaatgatgc cagccttggt
    13261 gcagcggaag taaataatga catgaccacc cttcgcgatg tgattggtct cgaaacaatc
    13321 agttggaaag atatctggga atacctgcgc ctgggacaac gggaactggt acatgaacga
    13381 aataccaatg aaacgaagat ccgtattgcg ttgaacctgg atggacaagg taaaaccaac
    13441 atccgcacag ggcttcattt ctttgatcat atgctggacc agctgggccg gcacagtggt
    13501 attgatctgg ccataacggt agacggagac ctgcatatcg acgagcacca taccatagaa
    13561 gataccggta tcgcgttagg agaagccatt tccctggctc ttgggaataa aaaaggcgtg
    13621 gctcgttatg gtttcacttt gccgatggat gatgcgctgg cccaggtagc gctggatttt
    13681 ggcggacgca gctggctggt atggacagcc gaatttagaa gagaaaaaat cggggacatg
    13741 ccgactgaaa tgttttatca tttcttcaaa tccttctccg atgccgcaaa gtgtaatctg
    13801 aacatcaaag tggaaggaga aaatgaacat cataaaattg aatccatatt taaagcactg
    13861 gcgaaaacca tgaaaatggc cttgaaaaga gatccggaaa atatgcaact acccagtacc
    13921 aaaggagttt tataatgaag accgtcatca taaaatataa cgcaggaaat atacagtcgg
    13981 tcctcgttgc cctggaacgc atcggggtcc aggctgttgt aacggatgat ccggaggaaa
    14041 tccttgcggc ggaaaaggta atttttcccg gtgtgggtga agccagtaca gcaatgcaat
    14101 acctgaaaga acgggaactg gataaactca ttgtttcact gaaacagccc gtattgggaa
    14161 tctgcctggg catgcaattg ctttgtgcgt attccgaaga aaatgacacc ccttgtctcg
    14221 gtgtatttga agaggtgccc gttcgcaaat tccggaatga aaaaaaggcc tttaaagtcc
    14281 cccagaccgg atggaactcc atacagcaat taaaaggacc tcttttttcc caggtaaagg
    14341 aaaatgaata ctgctatttt gtccacagct attatgccat attgaacgcc ttcaccaccg
    14401 gtactacgga gtatattcat ccgtatagtt cggcactgca acgaaacaat ttttacggcg
    14461 tgcaatttca ccccgaaaaa agtgccaaag ccggcgaaca gattttaaaa aactttttaa
    14521 cgctatagac gatgacgatt atacccgcca tagatatcat tgacggaaaa tgtgtgcgcc
    14581 tgacaaaagg cgattacgaa cagaaaaaga tatataacga agatccgctg gaagtagcac
    14641 ttcagttcga gggcgccggt ctgaaaagac tccacctggt agacctggac ggcgccaaag
    14701 ccggtgaagt aaagaactgg aaagtgctgg aaaaaattgc cggcaaaacc aaactggcca
    14761 ttgatttcag tggagggatc agtacacaga aaaacgtgga gatcaccttt aattccgggg
    14821 cggcctttgc cgcagtaggc agtattgcgg taaaagatga attcactttt tccgggtggt
    14881 tactggcatt tggtgctgaa aaatttataa ttggcgccga taccaaagaa ggcaaagtcg
    14941 tgatccgtgg ctggacagaa tccactaccc tcaccgtagc tgaactgatc ggacgataca
    15001 aaaccaaagg ggtgaaacag ttcttctgta ccgatgtgga gaaagatgga ctattacagg
    15061 gaacggcaat agaactatat aaaaaaatac tgaatcagca tccctctatt gacctgattg
    15121 ccagtggcgg tattactgaa gtagcggaac tggatgaatt aaggcaggcg ggttgtgatg
    15181 gcgccattat tggtaaagcg atctatgaaa acaggattac actggccgat ctaaaacgct
    15241 ttttataaaa tgctcactaa acggatcata ccctgcctgg atattaaaga cggacgtacg
    15301 gtgaaaggaa ccaatttcct ggaactccgt gatgccggcg acccggtgga actggccatg
    15361 cgctattgtg aagagggggc agatgaactt gtattcctgg atatcacggc caccactgaa
    15421 aaaaggaaaa cacttgcggc catggtcagg gagattgcca cccatatcaa tatccctttt
    15481 accgtaggtg gtggaattaa tacggtggaa gacgttaggg tcttgctgga taacggggct
    15541 gataaaatat ccgtgaatac ctcggcagtg aaacgcccgg aactgattaa tgaactggct
    15601 atgcagtttg gcagtcagtg tgtggtggtt gctattgatg caaagctgat aaataatacc
    15661 tggatggtcg tcacacatgg cggacgcacc ccgactttat taaaagcatt ggactgggcc
    15721 cgggaagctg cagcgcgtgg ggcgggtgaa atattactga catcgatgga aaatgacggt
    15781 acgaaaaacg gatttgccct gggtatcaca aaaaccatca gtgaagcagt agccattccc
    15841 gtcatcgctt caggtggagc cggaacgatg gaacattttt ccgatgtatt caaaaaagcc
    15901 aatgccgatg cggccctagc agccagtatt tttcacttcc gggaaatacc ggttcaggaa
    15961 ttaaaacgat atttacagga acgacagata ccggtacgct tatgatactg atcctgttca
    16021 tatccctttg tattgcctgg tatttttacc agtattttga aaacaaacga atagaccggc
    16081 tgaaggaaca gcaggagaag cgaaaagatg cttacgaaca actccttaaa ataatcagcg
    16141 agaaggaaga taacaagcat acgaatactt aatcaaacga atactatgca accggacttt
    16201 tccaaataca acgatggcct tatacccgtg atcatacagg atgcaggaac cttacaggtg
    16261 ttgatgctgg gatttatgaa tgaagcggcc ttcctgcaat cccaacagga aggtaaagtc
    16321 actttttaca gccgcagcaa acagcgtttg tggaccaaag gagaaacttc cggcaatttt
    16381 ctgcatatac aggagataaa agccgactgt gataatgaca cgttgttgat ccgggtactg
    16441 cctgcaggcc cggtttgcca tacgggcact gacacctgct tcaatgaaaa aaatccaccc
    16501 ttcagtctgc aacaactgga gcggataatc attgaccgga aaaataatcc ggatgaaaaa
    16561 tcctacacgg cgtcattgtt tgcaaaaggc attaataaag tggcgcagaa agtgggggaa
    16621 gaagcggtgg aactggtgat tgagagtaaa gatgacaacc aggaactatt tttaggggag
    16681 gccgctgacc tgctttttca ttacctggtt ttactgcaac acaaagggtt cacactcgcg
    16741 gatgtggtcc atacacttga aaaaaggcat aaatagattt ttttacagga tcggtatact
    16801 gactattagc tggcaacctt cacctgcagc cgttttgata tcagcggcac cgtcataaaa
    16861 gcgcacccgc tcataaatat ttgacaagcc aatgcccctg aaggtatggt tggcttcaaa
    16921 gccgatcccg ttatccttaa cgagcatccg gacccactga tcatcacttt caagtaacac
    16981 ctccacacgg gtagccgaac tatgcttcaa aatatttttc aattgttcct ggagaattct
    17041 gaataaagta acttttttcc cttcggacaa taagtcagcg ctcagatcct gtataaaatg
    17101 aatatcaatc ttactggcca tccgggcatc ttccagtaac tggctgatac aggaaataag
    17161 attattctct ttcaacctgg gcgccaccag ttccttcgat agtttccgga tttcttcaat
    17221 agccaggctg atataatcca atcctttcga ctggtacaac cgctgatccg cagcatccgc
    17281 ttgtatcatt tcaacaaaca gtttcgcaga tgacagtatc tggttaacat tatcatgcag
    17341 ttcatgtccg atctgtgtcc gttccttctc ctgggcctgg atcaccagtt cagaaacgtc
    17401 tttatgcctt ttcagccgtt cttctatcag cattttttcc agctccttga aattgcttat
    17461 atcctgaatg gatccgatca tctttaccgg cagcccattt tcataaacaa tgtacccttt
    17521 atcccgcatg tgcttatatt ctccatccgc acatttaaac cggtactccc cttcccagga
    17581 ttgctttcct tcttcagtgc tttgttttac ggcatccccc acccggttcc ggtcttccgg
    17641 atgaatgcgc ctgagccacc aggaaagtcc tttgggtata tcggtatgat aaccgatcaa
    17701 atccatgagg gcctcattgc ggaaaatctg accggtttgc atatcccatt cccagattgc
    17761 atcactcgtg gcgcgggata aaagcaataa acggtcattg gtttcccgga gtcgcttttc
    17821 cgcattaaat ttttcggtca ggttaacagc atggccaccc aatattttcc ttccggaaat
    17881 accctctatc gggaaaatat caatatggaa tacataatca gaaccatcgg cccatttaat
    17941 acgctggata tggtcctccg atataccggt ttccaatacc cgtaggtgga tcccaagcag
    18001 tgcttcggct actgctgggg gaaccagaga aagaatactt ttgttcgggg catttatttc
    18061 atctattcca aaaaattcgt aaaaatggcg gctggcaaaa agcaggttgg actgatcatc
    18121 cactacccat gcaaggttcg gtgtttgttt catgaaagaa cggagtacag cttttcgttc
    18181 tttaagttca cgtatcagct ttttctcaag ggtcaggtcc gtgatactgg aaacaaccgc
    18241 aaatgggata ctgctgtgat cacggaacaa gggctgtgaa ctcaggtgca gccagcgctg
    18301 ttccccgttt ttcaaacgga taaccagtac ctcggtatgt cttttccggg tttcaagggc
    18361 cttaataaat gatgtatctg caaacaaaac aggctgatcc tgttcgttcc ggatatccca
    18421 tgatttattc cattgaatgg cgatccgggt caggttgtac aaatcctcca gggaaatatt
    18481 caatatctct gctgtcttct gattggctgc aatgatttct ccccgggcat cctggaataa
    18541 tatgccggcg tccaggctgt caaaaatgag ttggtaatta tattctccta accggtagaa
    18601 tgcacgggta cggtcctcat ccagtaattt atatccctgg catagatagg ctcttgattc
    18661 cccgttcgcc ggcatcgggg tgatatgcca tttcatcgga tgatataacc cgtttttcag
    18721 gtaggcttcc acacaactca cggtcccctc tttatccgac tctgacaggc gttcagcgaa
    18781 actcttcctg ttaaccggat gcaaaaggtc aaaaagattt aacacgttat ccttacaact
    18841 gctcaggtgc agcgaacggg tcattcttga atttgcagaa aggatcgttc catcttccct
    18901 gatcagcgaa atatattttt cccgtgaaag ctgcacttcc ccgataactt tttttaaatc
    18961 actccactta atccgcatac tgatctgatt ggcttgtttc aatattcttc ctgcaatacg
    19021 cattaacgat ttccggcacc tgtaaaaaat cattcgactt atccagaaaa tgctgggcgc
    19081 cgagttccct gcactgttcc ctgtaatacg cttcggcatg attggtcagc attatcactt
    19141 ccgttccggg attcttttcc cggatatagc gcagtaattc aataccgctt ttccctggca
    19201 tattaatatc cagtaacagg atatcctggc cgcctgctgc catttcctgc atggcaccat
    19261 taaaatcgga agcaatgctg attttttttg gatacttaac gtcgtcgaga atactgatca
    19321 ttcgtttgat gaaattcaga ttatcatcca caatcagtat gttaagtgtg tttttcagga
    19381 caggaatcag gttggtatta cgatacaaat aaaagaaaag ccccattaag gggctgtaat
    19441 acaatggcca aagatttgta gcctttagtc tacaagtccg ggtttaaatc aatctcgatt
    19501 cgatggcata acgggccagt tccgcattac ttttcatttt cattttttcc atgatccggg
    19561 accggtaggt actgaccgtg gttgagctta ataagagctg gctggcaatt tctgtaatgg
    19621 tctttccgga agccagcagt ttgaatacat caaactctct gtcggaaagc acctcatgta
    19681 atggcttttc cgtctcatga ttaaccgact ccgccagctt ttcagccagg gacagggtaa
    19741 tatagcgttt ccccttgatc actgtttcaa tggctctgac aatatcatca tgtatcgttt
    19801 ctttggtcag gtaaccggcg gcaccggatt tgagtaccct cagcgcatat tgatcttcag
    19861 ggtacatact cataatcagc accggtagcc caggcgctat ttcctttatt tgcatgatgg
    19921 catccaaccc gctacggcca ggcatattca tatcacatac cacaacatcc cagtctttat
    19981 ggaggacgat ttcatgaatc agttcctcta catttgaggc ctccccgata cgggcaaaag
    20041 gatattgttc cagcaataat tgcttcattc cccttctgac aatagcatga tcgtcggcaa
    20101 ttaatattcg gatcataatg aatcctgttt atctgcaaat ttaaataacc ggtaccgaaa
    20161 caatcacaga tgttccctgg cccggcaaac tgtttattat ataagtcccg ccaatcattt
    20221 cagtccgttc tttcatacca agaatgccca gcgttttttt ctctgccact ttcatcggat
    20281 caaacccttt cccattatcg gcaatagtca ttaccaggca gttatcggcc tcttttactg
    20341 aaacagccag ctttttcgcc tctgaatggc gtaccacatt ggtcaatgat tcctggaaaa
    20401 tcctgaataa tgcagttttt gcggcttccg gaactggtat ttcatcctgc ggcgcttcaa
    20461 aactggtttt tatgcccgcc cgtttttcaa attcaccaag ttgccattcc atcgccgcag
    20521 tcagtcccag gtcatccagt aaactcggtc ttaactggga cgaaatccgt cttacagact
    20581 gaaccgtttc atccaacatg atcagcaggt ctttcagccg ctgatttact ttatcgtctg
    20641 tcccctgaaa acgtttatta agccaggcta catccatttt caacacggta agctgctgcc
    20701 ccaattcatc atgaatctcc cgcgcaatat tggttctttc ctcttcccgt atattctgaa
    20761 ggtgggcagt caggcttcgc accgcattat acgatgattt cagttcttct tccgctttaa
    20821 tacgctctgt catatccctg attaccgaaa ggaaccggcc atccggcaat tgttgtgaac
    20881 ggacttctgt gatcaccacc gtaccatcct ttcggcgcat ccggcgaatc tttaccgttg
    20941 aaatacctga actcagcaca tcaaactgaa cggggttcac cactatatct tccggtaaca
    21001 ggacatccga taatttcatc tggctgaaat cctccctgga ataacccagt aatcgggagg
    21061 ccgaggtgtt ggtatccagc aatattccat ctgaatcata caaagcgatc gagtccgcgg
    21121 cttgttccac cagggtcctg tacttttcct cactgacccg gactgcggtt tcggcctgtt
    21181 tcctttcagt aatatccttg gtgactccaa gtgcactgat tattttttta tccgtattca
    21241 aaaatgggac cacacttaac tcacaccatc ttttcatccc ctttaatgta atcatctgga
    21301 attccaacgt accggacttg cccttaaaag cgtcttttat cagccggatg gcctgttgct
    21361 gttgtccttc atctatcagc ggcaacaccg atttcccttt aaccatctca atacttgctg
    21421 cttcaatcat ctgtaatcct gcccggttga tatccagcag acaggcatct tcatccatta
    21481 atttgataca ctcagggtcc gtgtccagga tggtccgtaa acgcatttcg ctttcttcaa
    21541 tcttacgctg aacggtataa atgggggtca tatcaatcat ggaaccaatc attcgaatgg
    21601 cctgtccacc cgggtcacgc atgataaaac cacgatcaag aaaatgcagg taactgccat
    21661 ctgctttccg atatcggtac tcatccctcc agaccgaact cttgccttta aacgttttat
    21721 tggcattcag acggacccgt tcccgttcat ccggatgaat acggtcatac cactcatttt
    21781 catccaccaa ttccgatgtt ttcctgtatc caagacttga ataatagtta tcattccacc
    21841 agagttctcc agccagcaga ttccagtccc agaccagatc actggttgcc ctggaaacaa
    21901 tctggaaacg tgcatttgac tccagcaggt ctgtttcagc cttgcgttgt tcagtaatat
    21961 cttccgcata gccatattcc atccggcggc catccgctcc actcaccaca tttattttaa
    22021 cagcgatcca gcgaatatct ccctgtggcc ggatgatccg gtaattgaga ttgaattgct
    22081 ttttttccgc aatccggtga tatgcttcta ccagtttctt caggtcatcc gggtgtactg
    22141 atttgacaaa gtcagcagga ttccggtaca ggtcttcaca tttacggccc cagactgttt
    22201 catacgacgg actgatgtac aaagtgtgtt gtgtctcgag gtcgttaacc caatagacac
    22261 ctgaaatgtt ttctaccagg ttctgaaaac gtttctgggt gcggatcagt tcctcttccg
    22321 catttttcag gctgttgatg tctgtatgtg ttcccaacat acggagcgca ttcccctgat
    22381 catcatattc gagtattttc ccaatagaaa gaatccactt ccatttaccg ctttgggtac
    22441 gctgcctgaa ttcggtccgg aattctcccg ttatccctga aacataatcc gcaaaggctt
    22501 tttgggcggc cggcaaatca tccggatgca tccgttccag ccataattca ttgttctcgt
    22561 taaaatccct gggatcatac tccagcatca acgcatattg ttcattcaca atcgcctctc
    22621 cggtttttat attcaaatca tacaacccct gctttgcagc ctccatagaa agtcgcaggc
    22681 gttgctcact ttccatcaac tgctcttccg atcgcttccg gtcgctgatg tccatcatac
    22741 tcccggtcat tcgtagcggc tttccggcat catctctttc aatataacag cggtcaaaca
    22801 aataaacata ttcattctcc ccgtttaaaa aacgatattc agaaatgaat acattttttt
    22861 cagaggcaag ggtggattcc tgccggctga caatcagggc gcggtcatcg ggatgtatcc
    22921 ggctccgcca aacatattct tccggcaccg ggtcttcaat tgtcaatccg tatagttgct
    22981 ggtgattttc attcgcccat tttcttccgg tcagaatatt ccattcccag atcgcatcgt
    23041 tggtggttcg ggaaatcatt tcaaaccgtt tattggattg acggatctct tcctcgatgc
    23101 gtttccgctc ggtgacatcc cggatgatca ggctggtacg ttctctgcca aaagcatctt
    23161 taaaaatggc tgaactgaat tctcccggaa acttcgttcc atctttacgg acaaaactca
    23221 gttcaccggt cagataaccc attctttccc gtaagtccag caatccatga atccgttcat
    23281 cctcctgatc gatcagaccg gcccggccaa gggcaacgat ttccgcttcg gacatgccaa
    23341 acatctggca ggctgcggga ttggctgaaa ggatagcgcc atccggtgaa gaaagtaaaa
    23401 ttccatccat actgtattca aagaaagaac ggaacttcgc ttcactttcc ttgacctcat
    23461 tctccgcttt tttaatttct gtaatcaacc ggccttccat gatcagcagc atcggtttac
    23521 catcaacacc gggaatgggt tttatagaca aatccaccac ttccacttct ccctctttca
    23581 gtgaaatttg tccctcatac cggatcagtt cgccagccgc agcccgggtt attgcttctt
    23641 tcaccttttc aattcttgct gtccgctctt cttcattttg ccagagtcgg atatcccaga
    23701 aataccgtcc gattacttcc tcattccggt aaccggttat agcgagggct gttttgttgg
    23761 tttccagtat ccggccatct ttatccagca aggtcaccat attcagctgt gtattgaaaa
    23821 tggcccggaa ccggttttca ctttctgtca gcgctttttg tacctgcatt cgttctgaaa
    23881 tatcccgtcc aattccaatg aaatgaccat tggatagcat ctttgtactg agctccagtt
    23941 caattaccac cccgtctttc cgtcgcactt tccggttact caggatactg gcacctgtgc
    24001 gcagttgttc aaatctgagc ggaggatctc cgggttccag taccagaata tccatcagac
    24061 tcatcaacat tacttcttcg cgtgaataac ccaacagacg aacaccggct tcattcacct
    24121 gtataaaatg cccccgttca tcaacgagta caatggtctc tgtcgcctgt tctaccagtg
    24181 tcctgtacct ggattcactt tcctgcaatc cggcttctgc attttttcgg gccgaaatat
    24241 ccctggtgcc tacaaatgca cctgtaatct cattaaattc attacgcaca ggggttaact
    24301 gaatactgac ccacctgagt tgctgaccag attccacccg ggtttcatat tcgatagttt
    24361 ctccgccaaa agccctgttg taactttcaa gaacaacttc ttttctattc tccggtataa
    24421 aatcaattat ccggtctcct gaacgaatga catgtttcca taccgattcg aggttcactt
    24481 ctgccatccg gctcattaag atcacacggt aatccagatc gatcacatag aattcaccag
    24541 ccgcgctgtc cagcacctgc cggagttttt cctctccttt ccggatatct tctgttttct
    24601 ctttaacttt tgcagccagc tcatgattga attcatatat cagcttttca gccagtttcc
    24661 gttgcgtaat atccctgacc acagatacaa aaccagtcag tttgccatga acatccctga
    24721 gagcggttgc agagatgtga caataaatac ttttcccgtc tttcgtgaaa tcattcgact
    24781 caaattcaat cccgcccttg atggcaattt cccgctgaat atcctgatct ccattttcaa
    24841 gtgcactgct ctgtatcagt tcactgattt ttctcccgat tgcctcttct gaggaatagc
    24901 cataaatatt gtgtgctcct ttgttccagc tgattacaat ttgctgaggg tcaatagaaa
    24961 taatcgcatc actggtacgc tctgtaagct ccgcgaggta ggcgatacga gcttcatatt
    25021 gctcttttcg ctttagatcc ctgtggatga taaaaaagat taaggaggtc aggagtatga
    25081 aacatatact cagtatgaca aaaagccggg cggtatttgt agccgcgatt tcacgactac
    25141 tattagagga attcagcact ttgcggtcag ttgtttctaa ggccagcacg agattccgga
    25201 tactgtccat aatctccatt ccggtccctg accgaaccaa caacaacgca gaatcaactt
    25261 tattctttga aactaacgct tctgtctggg ccgcaatttt tagttttttg tttgtaaggc
    25321 ggacgaggat ctttaaagaa tccttccggc tttctgatat tccatccaga cttaatatgg
    25381 cggtagtatc tgtagaaaga ttggagatag ccgcatcata aggtgtaaga aaatcgcggt
    25441 tagcggagat caggaaacca cggttggccg tttcgatatc ctgcacatca tccatcaggt
    25501 tttccagaac tttcagactt tgtaatgtct tattgacctg taaagtctcg taccgtgccc
    25561 ttttcatatt tacataggaa taccatgcaa aaaaagagac cagcacaaaa gtaatcacca
    25621 cagcgatggt aatactctga cgggtataaa ttcgattcat gtgaaccgta ttgctttact
    25681 tcaggaaaag cacaattagg caactatcag agtccgtgaa catttatgaa gttgacaagg
    25741 gcagcggtat tcttcagact aagtttcttt aatatattat accggtgaac ttcaaccgtt
    25801 ttcagggata tatccagtcg tatcgcaatc tctttggacg ataacccttc tttaattagt
    25861 tgtacaatat ccagttcgcg ccgggataat acattcatat ccggtgtgcc cccttcatcc
    25921 tcaagctcct gttgcgccag gatatttttc acctcttcac aaatatattt tttaccacca
    25981 tatacttcca cgattgaatt aatcagttca tccttggaag aatttttggt aacatacccc
    26041 attgcgccga tctgcaacat acgccttgca taagccggca tggaatgcat agaaatgccg
    26101 atgatcttgg aacccggtga aaactttcga actaacttgg tggcttcgaa accgttaacc
    26161 ggtgtcatgt tgatatccat cagcacaata tcgggttttt tggtgcgggc gatttctacg
    26221 gcttcatcag cgttactggt ttcgccaata acctggaaac ggctgtcgct gtttaagata
    26281 aatgaccagg aatcccggat cagtttatgg tcatcgacaa ggagaatagt aatttttccc
    26341 atacggatag tggtttgcgt tgagcggtca aggttttttc cattgaaaaa ttaagtaaat
    26401 tttcaatgaa acctgcataa atcattgtct tatagccctt ttattggtat aaaaaaatga
    26461 attttaagta aatcccctta gtccgtatat gtagttttac gatttccttt agcttttaac
    26521 ttaattgact aaaccgaaac tgcttaagaa aaagtaatga ttacccctca tcgtaagtca
    26581 ttttacgagc gattccatac tccagacctc tcagttctgc cagccctctc agtctcccaa
    26641 tcgcagaata ccctggatat gtttcctttt gaagatcatc cagcatctgg tgtccatgat
    26701 ccggccgcat gggtatcgaa caatcccttc gttgctggat ccgtaatact tcttttataa
    26761 tttcatacat gtcggtatcg ccggccaggt gatccgcttc ataaaaattc ccttcagcat
    26821 cccgtttggt atttcgcaaa tgcaaaaaat gaacccggtc cccgaaccgc ttcaacattt
    26881 gcagtatatc attatcagcc ctcgctccga aagaaccggt acaaaaacat aatccattcg
    26941 ccgttgacgg ggctgctgcc agtaattccg ccgcatcttt ttctgtactt acaacacgtg
    27001 gtaaaccaag taccggatat ggcggatcat ccgggtgtat cgccatctgt aacccaagcg
    27061 tctcagctac aggaatcact tcccgtaaaa aataaaaaag atgttgcttt aaccgggctg
    27121 catctatccc tttatacgtt tcaagggcgg tcagaatctg ctcttccgta aaccggtctt
    27181 cactgccggg caatcccagc aaggcattat gaaacaatcg ttcttcttcc tcatcggtca
    27241 tccggtcaaa cctgaactgt gcttttcgta tttccgctgc tgtatagtcc ttttccgccc
    27301 ccgggcgctt caacataaaa agatcaaagg ccacaaaagc agcacgctca aaataaagtg
    27361 cccggcttcc atcgggcatc aaccaagcta tgtctgtacg catccagtcc agtaccggca
    27421 tgaaattata cgttaccacc ttaagtccgc aagccgccac atgccgcaag ctctgcttat
    27481 aattttctat atgttttaca taattatcct gctgcttttt aatatcctca ctcacgggca
    27541 gactttcaat caccgtccag gtcataccgg cttcttcaat cagcgctttg cgttttagta
    27601 tctcctccat cgtccatacg tccccgacag gaatatggtg aagggctgtt accaccccgg
    27661 tacatcctgc ctgacggata tccgctagac taaccggatc attgggcccg aaccaacgca
    27721 tggtttgttc cattcttttc atacttgtat cattcatttc attaaaatgc ctgcaggcct
    27781 gaaattcagg caatagctgc aggcatattg atctgtttta tttcaatccg atcagatata
    27841 tcggttgatc aggttttcca ggaattcctg tttaccgctg atcactgacg gctctccgtt
    27901 ctccaacgcc aaactccgca gttcttcaag tgacaatttt ccggtttcaa aatcctttcc
    27961 tttcccatta tcaaaagaag aataacggtc ggcccttact ttcttataat ccgattgctg
    28021 taaaatgtta tccgcggtga tgagcgcacg cgcaaataca tccattccgc caatatgcgc
    28081 ataaaacagg tcttccggat ccgttgaatt ccggcggatc ttggcatcaa aattaattcc
    28141 gcctcccttg aagcccccgg cttcaagtat gaccagcatc gcttcggtca gttcattgat
    28201 atcattggga aactgatcgg tatcccagcc attctggtaa tcgccccggt tggcatccat
    28261 actgcccaac agacctgcgt cggccgccac ttgcagttca tgtgtgaaag tatgtccggc
    28321 cagggttgcg tgattcactt caatattcag actgaaatca ttcagcaggt catactgacg
    28381 gagaaaccca atcaccgttt cacaatcata atcatactgg tgtttggaag gctcacaagg
    28441 tttgggctca ataaaaaact ttccgttgaa accctgtttc cgcgcatagt cttttgcggt
    28501 atgaagaaaa cgggccaggt gctccttctc tcttttcata tttgtattga gcagactcat
    28561 atacccttct ctccctcccc agaatacata gttctcccca cccagggcaa tcgttgtatc
    28621 cagagctgct ttcacctgtg cggcggcatg agtcagtaca tgaaaatccg gattcgtggc
    28681 cgccccgttc atataacgac ggttggaaaa caggttagct gtaccccata ataattttac
    28741 cccgctggct ttttgctttt cactgaaata ggcggtaatg gcctgcaggc ggcggtcatt
    28801 ttcattgata tcattggagt aatcaaccgc gtcaacatca tgaaaacaat agtaaggaag
    28861 tccgagtttg gtaatgaatt caaaggccgc atcggctttg tcttttgctc tttccaccgg
    28921 atctgttttc tcactccagg gaaaaagatg ggtgggttcc ccaaacggat cggccccgct
    28981 gccacagaag ctatgccagt atgcacaggc aaaacggagg tgttccttca ttggttttcc
    29041 ggccactatt ttgttttcat cataccatcg gaatgcgagt ggattatccg acccaagacc
    29101 ttcgtacttt atctggccaa tccctttaaa atattccttg tttcctgtaa cgactcccat
    29161 gatagtttat ttaaataatg atagaattaa ttaaggttct tttccagttg ttccagccac
    29221 tttgcgtaaa gctcatcata ttgatgtccg gcagcaggac gtatggtttc caccggtgca
    29281 aaattactaa aagcttcgcc cggattttta taaaaacccg ccccgatacc gctcccgata
    29341 gccgctccgt ggctgccatc gcactgatat aactccaccg gcacattcag ggtatttaca
    29401 aaggcggaag tgaatacatc actcaggaac atattggctt tgccggccct gattacccct
    29461 ggctgcatgc cattttgccg cataatatcc agtccgtacc ggaaggcaaa cacaatacct
    29521 tcctgtgccg cccggtatac atgcgcaata ccgtgcttat tgaaatcgat atgcaggaat
    29581 tgggcattca tcacccggtt gttcagcatt cgttctgccc cgttaccaaa aggaaatacc
    29641 atgacgcctt cactgccggg tgccactgct gccgctttat ggttcaattc cgcgtatccc
    29701 atcagatcgc cggatatatt cttcacccag cgattcagaa taccggtgcc atttatacat
    29761 aaaagtaccc cgagccgacg ttgttcccgg gtatagttta catgtgcaaa cgtattgatc
    29821 cgggattcat gatcataagc aagctgatca cttaccccgt aaatcacacc ggatgtacct
    29881 gcggtagcag ctacttcgcc cggctctagt acattcagtg aaagggcatt gttcggctgg
    29941 tcgcccgctt tgtaagttac cgggatacct acccgtaaag aaagggcccc tgccactttt
    30001 ggggtcagtt gcccatgctc cgaaaacaca tcccggatct cggggatcag ggaacgggaa
    30061 aatccataat agttaaacac atcctccgag aggccgtttt ccttaaaatc ccagaataca
    30121 ccttctgata aagcttctat acttgttgtt atggtaccgg taagtttcat ggcaataaaa
    30181 tcacccggaa gcatgatctt atcaattttt tcatacacat ccggctcatt ctcctttacc
    30241 caggccagtt tggacgcggt aaaatttccc ggcgagttga gcaggtgcaa caggcatttt
    30301 tcacggccaa tggattcaaa agccttatcc cccgtttcca ctgcccggct gtcgcaccag
    30361 atgatactat gccgcaacac ctgctgatct ttatccacgg ccaccagtcc atgcatctga
    30421 taagcgattc cgatagcccc gatgtctgcc gggttgtaag cccgttgggc atgtactttt
    30481 aataccgcca gttgtacctg ctcccaccag gattcaggcg attgttccgc ccacccgggg
    30541 tgtacggaaa ttatttccgc ctccgtttca gggtattggg cagtagccac ggtttgttga
    30601 gacgtggcat caataaccga aaccttaact gatgacgttc ctaaatctat tccgagcaat
    30661 aacatgtttc accttttaat aatagcacgc aagttaaatt aaaaataaag gagggatgaa
    30721 agaattcacc cctcgtgacg attgcctgtt tgaactgctt tgccaaaaaa agctttatta
    30781 caatcctgac ttttcagacg ggttttattt acattaaaaa ttacgaactc ccaaagaact
    30841 attgcggata gcattactgt tccaccaatg tggtaatact gcccgcaggc aacgtataaa
    30901 cgatactatt cccggcaact gaagccgtcg ccccttgcgc cacattttca gcagcggagg
    30961 tggtataggg caaaaaag
//
LOCUS       DAOPMW010000006        29116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1380_length_29116_cov_12.422009, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000006 DAOPMW010000000
VERSION     DAOPMW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 29116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29116
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_29116_cov_12.422009"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..374
                     /locus_tag="PLU11_00680"
     CDS             45..374
                     /locus_tag="PLU11_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN57646.1"
                     /translation="MKKIPIRQMGAAHQQGQLDHLVFKDGTRYSLKAIYTPSAFEQHC
                     KLPEQLGCLLTDEGYIQTGPGHETSIEGVYAVGDNASRMRTVANAVAMGNAAGISLSK
                     KMIVEQF"
     gene            472..1125
                     /locus_tag="PLU11_00685"
     CDS             472..1125
                     /locus_tag="PLU11_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57647.1"
                     /translation="MKLILVLTFAILNTVNLMATAQYPDKLIYKGKEYALHSNPLEDY
                     FDKYPDKRPKGGMMSSALWRGYVASFEFIDSQLYVKDIQIEVMDSSSKELYKTKWKTV
                     LKEVFPGDERIKTDWVTGILVIPYGKLVNYVHMGYGSTYEKYILLEIDKGDLKKEKKY
                     NYKKYELFKDRQFEAFRKTEEYQKLKTDLKKDGSTDEFVDSFLRSYITSYTTKILED"
     gene            1403..2566
                     /locus_tag="PLU11_00690"
     CDS             1403..2566
                     /locus_tag="PLU11_00690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPN57648.1"
                     /translation="MSTDQHPQPAEQNDSPSPTPEKEQQAAVPVKTNPVNDFISLFKP
                     AEGYFFTPVLLILNILVFVLMAVTGVSILDPDGASLVKWGANFRPVTLDGEWWRLITC
                     CFLHIGIIHLLMNMYALMYIGVLLEPLLGKTRFIVAYLLAGLLASLTSLWWHDFTISA
                     GASGAIFGMYGVFLALLTTNLIEKSARTSLLSSIGLFVGYNLLYGLKGGIDNAAHIGG
                     LVSGLVIGYALIPSLKNPGNNKLTNGTLSGLTVLALAVSFFVYTKLPNDISLYDKKIV
                     TFTELESKALNVYSMGSNTPTDTLLTAINKGLGYWGDNIKLLESLNTLKLPANLQQQN
                     QVLKEYCNLRIRSYNMLYKAVSENSDQYSDSLEILNADIEKKVSELVKIRDGK"
     gene            2673..3341
                     /locus_tag="PLU11_00695"
     CDS             2673..3341
                     /locus_tag="PLU11_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HPN57649.1"
                     /translation="MYHFSYFKEKDKQLILNFMEAHPFAFLSGCSLSGKPVATQIPVL
                     IEEREEELYIQGHIMRNTDHHKALVENPNALVVFTGPSTYVSATWYSNPQGGSTWNYM
                     SVHISGPIKFMSADELLEFMRKFTLKFEKGNRESLTIIDNLPASYVNKMLPAIAGFEI
                     KADKIDNVFKLSQNRDETSYLNIISKLEEQGGAGALIAAEMKKRKTELFPPGVEWDGS
                     KFDS"
     gene            3405..4634
                     /locus_tag="PLU11_00700"
     CDS             3405..4634
                     /locus_tag="PLU11_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPN57650.1"
                     /translation="MNRYIPQFLFVVTCIFLSGCTDTKKESYTIPSEFEEQEYIWLTW
                     VESGFLGGEPFKVTAMSAMKEITPYVKVRLFYGPQMGYDSAQLKSRIYESLIANNIDT
                     SRITLFYNEVYCGAIQDSGPVFLRNTEGRFAIADFNFLHPDNRTASIDKNVAAHFNLP
                     VISSTMISEGGAWQTNGRGTMLLVESVELDRNKSMTKKQIEEEYKRVLGVTKFIWLKK
                     GAKEEEWGKLENGKYGIGTSGHVDEFCRFVNDSTVLLAAVAPADTIGNEISKETYRRM
                     EENYKILLQSTDQDGKPLDIIRLPAGPLMSKNVAYKNLSKDEQSWFDQVTTDSVEFYL
                     ATGYMNFIIANQVVVAGKCWKEGLPFEFQERDKLAKSILEKAFPGRKIVQIDCMPLHH
                     DGGGLHCHSRNQPKGKL"
     gene            4703..7132
                     /locus_tag="PLU11_00705"
     CDS             4703..7132
                     /locus_tag="PLU11_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPN57651.1"
                     /translation="MNTLLKLIILFFSFAFTLISHSQPTPISKTPTGFTVACKDYLTE
                     LQVYSPGNIRVKKYPKEKTFNPQSLSVTAGPVSTPYSIKTKGTMVTLKTTELNAILDL
                     NTGALTFTSLNGTVLLREQPDGTRFTSFNDAGTPTLSVKQTYLLDTDEAIYGLGQQQK
                     GKMIQRNLTLKMIQDNTDDYVPFVLSAKGYGIFWDNYSPTVFSDDPSATSFSSEVGEG
                     IDYYFIKGTNADAVIAGMRTLTGASPMLPLWTFGFWQSKERYKTQDEPVEVVRRYRQL
                     GVPIDGIIQDWQYWSSNYLWNAMEFLNPDFGNPKKMISDIHGLHAHIAISIWNSFGPE
                     TKQYRELDSIKALMNFGTWPLSGVDKWPPLREYPSGVKVYDPYNPQARDIYWKYLSKM
                     HALGLDAWWMDSSEPDHLDFKDEDLNNKTYLGSFRKVRNAFPLMTVGGVYTHQRQVDS
                     SKRIFILTRSAFAGQQRYGANTWSGDVTASWNALRNQISAGLNFSLTGIPYWNSDIGG
                     FFLGQFRKPLANADYRELYTRWLQFGTFCPMMRSHGTDAPREIYQFGSKGEPVYDAIE
                     KYIRLRYRLLPYIYSTSWQVTHDHSSMMRALVMDFANDKKTLDINDQFMFGRSLLVAP
                     VTERQYAKATGVNGKDTIYTPDYSVTRSRQVYLPAGTNWIDFWTGTSHTGGQTIAKET
                     PLNIVPLYVKAGSILPMGPDVQYATEKKWDYLELRIYPGADGEFVLYEDENDNYNYER
                     GSYSQIRFSWNDKKKQLTIADRTGQFPGMLEKRSFRMVLVNEKQGAGDQPAGGDAHKV
                     EYEGKKLVISL"
     gene            7232..8131
                     /locus_tag="PLU11_00710"
     CDS             7232..8131
                     /locus_tag="PLU11_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57652.1"
                     /translation="MLTEEEILQTLDNYRNGHDPAFVQLGHPYVFPIDSRINVFRNTQ
                     GKWAIAIEKLGYDPRSEPIGLEITYYGNCLINLDYSETYFSNSYTVFPIDEESFKNTI
                     ELENLKPDAGFWIVRNNQIQLPHKKEEYLNAGIVLKEYEPNTISAEEVARLLVTRHRD
                     LFRATDEELYKSIPKDLDKILVIDEWHHRDFYQNNFNPIDDLPFTFPGPEELCRVIED
                     QIEKLNELNITKETLLNSFNLTEQNEIRLEQNKKELENNRPGSYETWQFIAKVIVTGD
                     PSHYKPTLAPTSHWKNWPDAGSL"
     gene            8217..8681
                     /locus_tag="PLU11_00715"
     CDS             8217..8681
                     /locus_tag="PLU11_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57653.1"
                     /translation="MPKLSLLFWTILLVLVIQSPAIAQTPNFTGTWIMNKEKSKIEDP
                     SEGMTGSVFRIKQEGNKFRIKIYHIYGEKEKKIGFKMKADGRTRKVKLIFKGKLEQKE
                     NSLIATMWRKNFLNIVNYKFGVNENELIADEVFTGRPRDHHSVWVFDREVVK"
     gene            9061..9789
                     /locus_tag="PLU11_00720"
     CDS             9061..9789
                     /locus_tag="PLU11_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57654.1"
                     /translation="MLQETIDRSYKVFAKYKPARPLDVCTNCCMTPEEENTLATLPLK
                     EITADFLAKYNDSAKPAKTSLDEVKYFLPRYFDLIGQFRFPSHSAELSFSRLAPFDKS
                     EWTVQEQELLKQFSIDFFNHCLRVYPIPAFNESIDCMLIMFWNSGFDISNLLTCWQNA
                     TGKESVFHFRDLYLHGFNQYNPAKLSNAFGEKELADLLRNWMQQEAVKKAFAEAIEML
                     ILEETQLEETDGYELNLLYDQLRA"
     gene            9896..10591
                     /locus_tag="PLU11_00725"
     CDS             9896..10591
                     /locus_tag="PLU11_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57655.1"
                     /translation="MVEDVYAEGDISKDTVFNGLIRFYDTTHNRLVMVASYKKGVLDG
                     PRTDYYKNGRPETKIKYENGKINGELIVYDTTGKITEKQNYYYDLVTGPSQSFKNGKI
                     SNYSFYSLDNEILFTLDYDKPKKIEKINNNSYFFWSINKYTTTTSPDPLNELFIYLPN
                     PPLLNLRYSLCIIDKAYTVLKVVKEFDSALYWEKTDLNFAGLKPTEMFALRLTIENAM
                     DDPADIAVMFKKL"
     gene            10595..11161
                     /locus_tag="PLU11_00730"
     CDS             10595..11161
                     /locus_tag="PLU11_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57656.1"
                     /translation="MKFILVISLLLFASCKNHIDVDNTEQREHGLSYIKGTNKLVDGK
                     VVRKSGGKMIELQSYKDGKMIGPFFQYGPDGQVVTKGFGIELKTYEQAIGGTDLTNCI
                     LSVVQVNNVFSYATVYMDNARVFDEKEKLLLLTKHILADYSATYKIDDLFIFDKNHEY
                     TVSKNAASKADYTVDTASRANPVKVNFR"
     gene            11190..12518
                     /locus_tag="PLU11_00735"
     CDS             11190..12518
                     /locus_tag="PLU11_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57657.1"
                     /translation="MLTGGLSAVVIAPGQTDSNNFMRPFFTILSTLVSVTCFTQVITV
                     ESVRVDSGIYEFKLQAKKDAADYSKAFRILSNQVKQNPLNAECRYFLGYAIDRLNAEY
                     GKDMINLKKEMTIKASEQFEEVNRLEPTYKGEIFILGPYAKLASIWGSLAASYLNRKL
                     VDSAVWAFTEGRKRGGFIDPILEFNRQLLNSCDKNAILVTYGDNITVPAWYLQTIENY
                     RTDITVVDANLIHTDWYPKFLKSERKLKISFSDAVIDTIDYIQWQPQQVTITNTVDTT
                     QVFSWELRPTYLDKYILKGDRILLDIFQQNFFSRPMYFYNESDSSFNLFLTPYLADEG
                     LVSRVMTKLIDYKKDVITVSKNLYDYNIDKLKKEDITKSDDAVSVLNSFRWAYFNNIY
                     HLLTQAKYDKAKELIKLMDNKFKKDKLPFTSVEVEKYFSELFKRVDKDYR"
     gene            13202..13876
                     /locus_tag="PLU11_00740"
     CDS             13202..13876
                     /locus_tag="PLU11_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57658.1"
                     /translation="MKILLLLLLPGLFADIHGQQATAKKNPVTVYLGAKTRVTPIYLK
                     RLHEGFEIGFPNNMEQPDYHLSGPGLLISEKKALGRKLEFSLHQVIRYDFLYQRMPMD
                     LVLPPDFSYSIKRKWIIDLEAELSHRFPRRKSVLLVSLGAGLNGLNTGFQETRRFYQS
                     PTSYTEKETRKNFLFPFLSTGFGWQKKKLLAGMKLGYCWDNPTVFASPFLYPELSVGY
                     RLFNGK"
     gene            13980..14570
                     /locus_tag="PLU11_00745"
     CDS             13980..14570
                     /locus_tag="PLU11_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57659.1"
                     /translation="MGLETHPSWYRDLPTLRTLILGSFPPHPSKRIYPFFYPNGRNRF
                     WKILADIAGMPLEWTRKDPVKAVEERYAIMQKLETGVQNIGYEIERKGTSALDTDIRI
                     TKYHDILSIITTHPELTRILLPGYSAEHSTTRSFLKYIEQKGIRSSDIPPIKAEAAFK
                     IYVNDREIECVILNSTSTAAKIRYEDLLEQFKHYLP"
     gene            14623..15558
                     /locus_tag="PLU11_00750"
     CDS             14623..15558
                     /locus_tag="PLU11_00750"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HPN57660.1"
                     /translation="MFRLLFILTLSLLLFSTCHVGADNTMLKKPGSIDSLHNAKQITA
                     FIHQIDSNYRKFILNDPVNYESRYAAFNYKRVADSLGVRPWSKADFDHNGLTDLLVIG
                     QHNDPSILIILNKGDHYQIIPITRRSFQDHTFPVVENNKIRYTFETNSDKIYQVLPAS
                     LETITLVYKFGDFIEENPLPAEHKIEKIEYNTTGCFGTCPVFKMTIRTDRSSVWEAIR
                     YNQFGSHKSAGTYSCTITEDKYAELTNLLNYIDFEKLQLDYAVRWTDDQQATLTITFD
                     NGQSKTILDYGLLGTYGLNRVHQLMFNLRENQDWK"
     gene            15570..16196
                     /locus_tag="PLU11_00755"
     CDS             15570..16196
                     /locus_tag="PLU11_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57661.1"
                     /translation="MPLKTYLPRHFNFPWFVPIALCCCLILLSCKQNKNPCNLPASVL
                     ADIAKLDSLVNVPALRERDSIWNAGGYKEAPVYSAQNETYRFIWNSAFNGSKVCRIEH
                     ISDSYNVTVKKFTKDGDIAEIRHFSISKKSWNNITDSLAILGFWTYFHPPYKMVFDPD
                     NWRLEAYKPIPDACTGKRYHSVSGSSPIDKGLYNMCKLLNRLDESLVE"
     gene            16265..17092
                     /locus_tag="PLU11_00760"
     CDS             16265..17092
                     /locus_tag="PLU11_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57662.1"
                     /translation="MFYLYRRNIISFGHNDGDEITDKHTILLDHASSASMIQSLRDKY
                     QHAFYKQLWCYKDLTAEERQVVLEVLGSKVTEDNWESELERISVAEYKKLEATLQFNF
                     TKIIEGNPQQDRFYPLIKHQQFSEFGLDSKYYHDMDHLPPLLISNNKQEAFEKGVLYL
                     LLTNDVSHYIGDPETTPYEADFRERFAAPRKKMLDYFSKSAFPFLKKSVSAELISRHQ
                     VLKFWILENPYFINTAQFEQFLKYKEEILKDKLDHFNRIVELFMEKKNELIEVINIY"
     gene            17108..17584
                     /locus_tag="PLU11_00765"
     CDS             17108..17584
                     /locus_tag="PLU11_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPN57663.1"
                     /translation="MTNEVHYTKGDATQPTGTGNKVIVHICNDIGGWGRGFVMAISSR
                     WKKPENEYRNWFNSAVNFSLGEVQFVQVEVETWVANLIGQHGIKRNNQNIPPIRYKAV
                     ADGLRKVADFCKPLNASVHMPKIGCGLAGGTWDEVEPIIISAFIPYGIPVTVYNFD"
     gene            17693..18469
                     /locus_tag="PLU11_00770"
     CDS             17693..18469
                     /locus_tag="PLU11_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57664.1"
                     /translation="MSSSNHFSDYYKTITATELLAILNNPGDYQPSAIEAAKAELESR
                     QLSETALQEARQPLVEKVQRKEAERKKIKAVEEKLKIAGTGFFEAINPIQQGIPSTEK
                     MIRRITIVFGVIFLYQLISDFREHIAYIKDFSSYPFLTVLYFFSLVLLPVALLFFWKR
                     KMLGWILLTIYLSFTVVTVLWFMFLTFTRKHSAYGVFENFYQTPPLTVFIIQLLFFVA
                     TMYLMCRKNIREVYSITDQKMMATISITALISFFLANAAN"
     gene            18646..19125
                     /locus_tag="PLU11_00775"
     CDS             18646..19125
                     /locus_tag="PLU11_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN57665.1"
                     /translation="MTPIETAIKMTLDRWYGSVASLDKVLNVLTDDQLLKETAPGKNR
                     GIYLLGHLVAVHDDMLTLLDLGEKQHPELTEPFIKMADKVTTELPSVAELRDYWSGQR
                     ALHREKFDQFTADQWFEKHTAVSADDFAKEPHRNKLNIIISRTSHLQYHTGQIALLK"
     gene            19132..19569
                     /locus_tag="PLU11_00780"
     CDS             19132..19569
                     /locus_tag="PLU11_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPN57666.1"
                     /translation="MSNQNFQYSFTTPKNPAEVFTLLLDPKNWWVGLFGETIEGKSQA
                     VNDEFTFSAGDGVHFSRQKLTELVPDKRIAWEVTESNLSFLKNTNEWAGTKICFDIEN
                     ENGKTKVLFTHEGLVPHFECYGGCSGAWTQYMEKLEKKLEARS"
     gene            19802..20194
                     /locus_tag="PLU11_00785"
     CDS             19802..20194
                     /locus_tag="PLU11_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPN57667.1"
                     /translation="MPTTILHPTFILRLAVAIILFMHSIPGMFNNGINDFGNHYLNEV
                     GFAPAGVPLAWAIKLSHVAAAICLLLNKYVKWAAVITILILVAGIVMVHFKEGWFVVG
                     GGSNGVEFNFLLIAVLLTFLFPNERKTS"
     gene            complement(20236..21243)
                     /locus_tag="PLU11_00790"
     CDS             complement(20236..21243)
                     /locus_tag="PLU11_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HPN57668.1"
                     /translation="MKAYIVKKYGKKEKLHLSDWAEPAVKENEVLVQIYSAGVNSLDA
                     LIRNGEFKLFLPYKPAFVNGHDMAGVVISTGSKVSRFKAGDEVYARVPDHHIGSFAEY
                     IAVHENALALKPTNLSMEEAASIPLVGLTAWQALIGIGRLEKGQKLFIQAGSGGVGIF
                     AIQLAKHVGAFVATTTSAKNMDLVKGLGADLVIDYKKEGYETILQGYDMVLHSSKEKG
                     SLDKSLQILKPGGQLISLVGPPTPEFATAIGLPWYLRLLTSLLSSGMKRKAKKGNIAF
                     SFLFMRADGAQLSQITKLIEAGVIRPVIDKVFPFDQTNEALAYVEAGRAKGKVVIKVK
                     E"
     gene            complement(21309..22106)
                     /locus_tag="PLU11_00795"
     CDS             complement(21309..22106)
                     /locus_tag="PLU11_00795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN57669.1"
                     /translation="MKQNILVTGASSGFGLLVATELHKRGHHVIGTSRNPEKYASKLP
                     FKLISLNLDSAESINSLAQSVFSEVKKLDVLINNAGFLVSGIAEETPIELGRQQLETN
                     FWGTIKVTNALLPQFRRQKSGKIITVGSIVGLVSFPNGAYYAASKHALEGYFKSLRYE
                     LKEFNINVAMIQPAAFKTNILDNSSTAVLKIDDYDLLRRKIEGFTNNLVAQAGDPAIV
                     AGKVIKVVETDNPKFRNIVGKGTSALIHLQHFAYGFLEKNVLKQLNS"
     gene            22200..22634
                     /locus_tag="PLU11_00800"
     CDS             22200..22634
                     /locus_tag="PLU11_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN57670.1"
                     /translation="METSKKRSDCPISGSLDVFGDKWSLLIIRDLMFGNKCTYNDFLK
                     SDEGIATNILASRLKGLEEHGIIEKSAHPDSKAKFLYRLTSKGIDLLPVLMEIYIWFD
                     KHYEMPADLKATMRDAKKDKEKFAKQIIKELRTRKSDNKSSS"
     gene            22756..24342
                     /locus_tag="PLU11_00805"
     CDS             22756..24342
                     /locus_tag="PLU11_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPN57671.1"
                     /translation="MKKLIHCFALLFVFSPFLHLRAQPPLIQNDTFWFTKEGQPIYSQ
                     GGGIFRFKDPSSGVEKYYWYGVRYREAETYRLNPAVTLPTSTFESVTCYSSIDLVNWS
                     FEHNILTMAELEKNGGYKRWLGRLGVAWLAELKKYVMVVQHGNQVLFTTSDTPAGNFT
                     WHNQISMKEMIGTPNTGDQTVFTDDDGKSYLIYSYGSGRNKIYVSEIGVKEGKANLLD
                     CKEIFRGESREGNCMFKYNGKYYMAASNIYGWDASLAYYLVADDIRGPYTPAKSMAVM
                     KGCEDDYAHITQTGFFVSVKGTKQETILYCGDRWADFAGNGLGYNQWFPMSFEGSTPV
                     FNSLHRWNLNAQTGLWSVDPGNNYVRNASFEADRRYVPTHVKPVQDQLTGWTSTVILG
                     NAISQDSLTSPVLNYFNTESDRKTVIGEKSLAITDKIKFNRKVSQTIISTPYVKLQDG
                     YYRLSAMIRNSGSFDTMMMYAKSKGKTFRYTITGENNEWKKINLPHIKVRKGKVEIGF
                     EAAGAAGAVWLVDDVELVRE"
     gene            24504..26606
                     /locus_tag="PLU11_00810"
     CDS             24504..26606
                     /locus_tag="PLU11_00810"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPN57672.1"
                     /translation="MPATSSKQLQIALAVLLFFPIVGISQVKPQIRTGFDAMYHIEQL
                     PLLFPNGTSTRQPLAYDPTGQNWDHHFMAAFTQYVDSIKQDDGTMVKEYVIYDEYGPG
                     VLYRQQMNAWYDRSKIPNGWLAWGEADQPRANANIRYYFDDEKEPRINMHLKDFFGTQ
                     KFPFTEPLAFVDSGYLFANLYYPFTYKKRLKVTMRPNAKSFEQMDTKWYQYTALSFPA
                     DYKLESWTGQSAVANPVIDQWRNTGDNPNNLEDSKKITSQTRIKKGSSATILSINQPG
                     SLAGFTLQLTPYNKETLFNTTLKIYWDDSKVPSIELPLGYFFGAGAKDYPATVDKVVQ
                     KSLTTLLFGFNPQTGTLYSYWPMPFWKSARIVIANNTDTDISNLQCTTWFKPAAILSY
                     NKNNTGYFLARRTIDSDPDTLGYRSVAFEEKGRGHVTGVVFYSDRYDMDGDEFCYIDD
                     SKTPQIHGSGTEDDHNQGWAGRAYQQPLWGGLVNGYDGAYRIYLNDCYIFNRNIQITY
                     EYSLTKPHFVNGGKTDVIVYYYKSATPGNLQLTDELDPGNHFSEQQHQYEVTGLTWKG
                     KLKDEYDGYERNLLYGAYTDDGKAYNGSSRFTVQVHPSNEGVKLRRRINRTGNGVQTA
                     LVYIDGKQVPRPWHIVIPSLSTGKGPVDGWYDSDFEIPSSFTKGKKSIEVKVEYVNSP
                     QKGAINEFYYWVYSYVGL"
     gene            26803..27717
                     /locus_tag="PLU11_00815"
     CDS             26803..27717
                     /locus_tag="PLU11_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57673.1"
                     /translation="MKSTLFTLFTLVCLQALAQPVTELRETEVYFRPTLTIPGKLRVM
                     YSADSSLLPAGHYKTSGGNRYHLFQVGDSGYLNGSFKDFTDQQVYREVTYLNGLVNRE
                     YFIMNGTVHTEAFDSTVTVLLYNPKKNKWNPELKSVRVEKEYGQKGRRLIISFTKGPY
                     NAYARYKYVDGILVSEMIPHYHEKKMDPSGKLYQLIQYNWTLKQIETSEFEKGILKSK
                     SILKNFSIVWDPKGIVHFTDDTGRFNDIVTFTYYDDQKIRKKEVEKNGKRTVTEYDKK
                     GKITSVNTYPLPTSESEIFEVAPVGEKQ"
     gene            27930..28415
                     /locus_tag="PLU11_00820"
     CDS             27930..28415
                     /locus_tag="PLU11_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN57674.1"
                     /translation="MLKSCWLFISLLGVALSAKAQLAKTDSLYLALKQQDSIFFERSF
                     NLCDLDYLDKTTHPELVFFHDQGGIQHKTEFLAGVKNNICGNPEQKPIRKAVANSLEV
                     FPLFSNGIFYGVIQTGIHEFYIRETGKPDRYSSRAKFTHVWLLDNGRWLLREVLSYDH
                     K"
     gene            28615..>29116
                     /locus_tag="PLU11_00825"
     CDS             28615..>29116
                     /locus_tag="PLU11_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57675.1"
                     /translation="MPFSYNFGEFPGQTINQGPKLISFSEYNGEADIELMCTQLDKKT
                     AKEQSRIVDEWCDHLTNDTLPLKSIWMETRISQKIFDAICKQKSLTSLWIKWGVYPDL
                     SAIRQLTNLEHLHLGNGKSITDLSPVGSLHQLKTLESDGLVNVQDYRFLSSLTALQDL
                     DIEGDGF"
BASE COUNT         8686 a         6708 c         6168 g         7554 t
ORIGIN      
        1 aattctccct tttttattaa attcattctc tgttaacagc caggatgaaa aaaatcccca
       61 taagacaaat gggtgctgca catcagcagg gacagctgga tcatcttgtt tttaaagacg
      121 gaacccgtta ttcacttaaa gccatttata cccccagcgc ctttgaacaa cattgtaaac
      181 tcccggagca actgggatgc ttgttgaccg atgaaggata tatccagaca ggacccgggc
      241 acgaaacaag cattgagggt gtatatgctg ttggggacaa tgcgtccaga atgcgtacgg
      301 tagccaatgc cgtggcgatg ggaaatgcgg ccgggataag cctgagtaag aaaatgatcg
      361 tggaacaatt ctgaagagaa aaaactcgtc ccggggttgt gctggctaca gccttttcct
      421 gtgtaatttt acaacagccg gcatcatgta aatacaccta aacctgaaca gatgaaactc
      481 attctagtct taacctttgc catactcaat acggtaaacc tgatggccac ggcccagtat
      541 cccgataaac tcatctataa aggaaaagaa tatgccctgc acagcaatcc cctggaagat
      601 tattttgaca aataccctga caaaagaccc aaaggcggaa tgatgtcctc cgcgctatgg
      661 cgtggatatg tagccagctt tgagtttatc gacagtcagc tatacgtgaa ggatatccag
      721 attgaggtga tggacagcag cagtaaagaa ctgtataaaa ccaaatggaa aactgtcctg
      781 aaagaagtat ttcccggcga tgaacgaatc aaaaccgatt gggtaaccgg tattttagtc
      841 attccatacg gcaagcttgt caattacgta cacatggggt atggctccac atatgaaaaa
      901 tacatattac tggaaataga caaaggtgat ctgaaaaaag agaagaagta caattacaag
      961 aaatacgaac tgtttaaaga ccgtcagttt gaggccttca gaaagacaga agagtaccag
     1021 aaactaaaaa cagacctgaa gaaagacggt agcacggatg agtttgtcga ctctttcctc
     1081 cgtagttaca ttaccagtta tacgactaag atattggagg attgactgct cctctctcta
     1141 ttgcaatcac aagcctcctt cactcagcaa tccctcccgg attcgctgtt ccttcaattc
     1201 agccaagcta tgaaaacaaa acaaaatgaa aatgccggag gtgtggttct tgataatgat
     1261 acaaaggata aacaacaatc atcgaagagc aacaatacca taaattcatt tatacaagaa
     1321 atataaaagg agtattttca tcttcaggaa atggagaaat tctatacgaa accagttacc
     1381 aaaacactca accctcccgt atatgtccac cgaccaacat ccacaaccag cggaacaaaa
     1441 tgattccccc tctccgacac ctgaaaagga acaacaggct gctgtcccgg tgaaaaccaa
     1501 tcccgttaat gattttattt ccctgttcaa accggccgaa gggtactttt tcacgccggt
     1561 gttgctgatc ctgaatattc ttgtgtttgt attaatggcg gtcacggggg taagtatact
     1621 cgatccggac ggcgccagtc tggtgaaatg gggtgctaac ttcagaccgg tcacactgga
     1681 tggcgaatgg tggagactta tcacctgttg tttcctgcat attggcatta tacacctgct
     1741 gatgaatatg tatgccctga tgtatatcgg ggtgctgctg gaaccactcc ttggtaaaac
     1801 aagatttatc gtggcatacc tgctcgcagg cttactggcc agtttaacca gcctatggtg
     1861 gcacgatttt accatcagcg ccggcgcctc cggtgccatc tttggaatgt atggtgtatt
     1921 cctggccttg ctcactacca acctgattga aaaatcagca aggacttccc tgctttcaag
     1981 tatcggcctc tttgttggat ataatttgct ctatggtctg aaaggaggta ttgacaatgc
     2041 ggctcatatc ggggggctcg tcagcggact ggtgattggt tatgcattaa taccttcact
     2101 aaaaaacccg gggaataata aactgactaa cggcaccctt tccgggctga ccgttttggc
     2161 cctcgccgtt tccttttttg tctataccaa actaccaaac gatatcagcc tctatgataa
     2221 aaaaattgtc acctttaccg aactggagtc aaaagcactg aacgtgtata gtatggggtc
     2281 gaacaccccc accgatacct tactgactgc cattaataag gggttgggat actggggcga
     2341 taatattaaa ctgctggaaa gtttgaatac cctgaaattg ccagccaacc tgcagcaaca
     2401 gaatcaggta ctgaaagaat actgcaacct gcgtatccgc tcctataata tgctttataa
     2461 agcagtttcc gaaaactccg atcagtatag cgacagcctg gaaatactga atgctgatat
     2521 tgagaaaaaa gtcagcgaac tggttaagat aagagatgga aagtagtgct gatatcggga
     2581 aagagggtca gttgaacatg ttctgtttgc cgcaagactg atccggaacc ctttatctaa
     2641 ttactcaact ttaaacaacc caacgactac acatgtacca cttttcatac ttcaaagaaa
     2701 aagacaaaca gctcatactg aacttcatgg aagcgcatcc ctttgccttc ctctccggct
     2761 gtagtttgtc cggtaaaccc gtggccacac agattcctgt tttgatagag gaacgggagg
     2821 aggaattata tatccaggga catatcatgc ggaatacgga ccaccataaa gccttagtgg
     2881 aaaaccccaa cgcattggtt gtgtttaccg gacccagtac ttatgtaagt gccacctggt
     2941 acagcaatcc gcaaggcggc tccacctgga actatatgag tgtgcatatc agcggaccga
     3001 taaaattcat gtccgctgat gaattacttg agtttatgcg aaaattcacc ctgaaatttg
     3061 aaaagggaaa ccgggagtca ttaacgatta tcgacaacct gccggcttcc tacgtcaata
     3121 aaatgttacc agccattgcc ggttttgaga ttaaagccga taaaatcgat aatgtattta
     3181 agctaagcca gaacagggat gagacgagtt acctgaatat tatttcaaaa ctggaagaac
     3241 agggcggagc aggcgcattg atcgccgcgg aaatgaagaa aagaaagacg gaattatttc
     3301 ccccgggagt ggagtgggat ggctcgaaat ttgattcctg ataaaaagct attaccgggt
     3361 tgcccaactt taatataaaa gtgtttaaca gtcaataata caatatgaac cggtatattc
     3421 cacaattcct gtttgttgta acatgtattt tcctgtccgg atgtactgat acaaagaaag
     3481 aatcttacac gatcccttcc gaatttgaag aacaggaata tatctggctg acctgggttg
     3541 aatcgggctt tcttggaggc gaacctttta aggtaactgc tatgagcgcc atgaaagaaa
     3601 ttaccccgta tgtgaaagta agattgtttt atggcccgca gatgggctat gactcagcac
     3661 aattgaaaag caggatatat gaatcgttga tcgcaaataa tattgacacg tcaaggatta
     3721 ccctttttta taatgaggtg tattgtggtg ctattcagga ttccgggcct gtttttctta
     3781 gaaatacaga gggccggttt gccatagccg attttaattt cctgcacccg gataatcgca
     3841 ccgcgtccat cgacaaaaat gtggctgcac attttaatct ccccgttatt tcttcaacca
     3901 tgatttctga aggaggggcc tggcaaacaa acggcagggg gactatgttg ctggttgaat
     3961 cggttgaact ggaccggaat aaatccatga caaaaaaaca gattgaagag gaatacaagc
     4021 gggtccttgg cgtaacaaaa ttcatctggc ttaaaaaagg agccaaagag gaagaatggg
     4081 ggaaattaga aaatgggaaa tacggaatcg gtacgagtgg acatgtggat gaattttgcc
     4141 ggtttgtaaa tgacagcacg gttcttttgg cagccgtcgc gccggcggat accattggaa
     4201 acgaaatatc aaaagaaacc tatcgccgga tggaggaaaa ttataaaata ctccttcaaa
     4261 gtacggatca ggatggaaaa ccattggaca ttatacggtt acctgccggt ccgctgatgt
     4321 caaagaatgt ggcttataaa aacttaagta aagacgaaca atcctggttt gaccaggtta
     4381 ccactgattc ggtcgaattc tatttagcca caggctatat gaattttatc atcgccaatc
     4441 aggtagtagt tgctggaaaa tgctggaagg agggactgcc attcgaattt caagaaagag
     4501 ataagctcgc aaaaagtatt ttagaaaagg cattccccgg aagaaaaata gtacagatag
     4561 attgtatgcc tttgcatcat gacgggggag gattgcattg ccattcgaga aatcaaccga
     4621 aaggaaaact atgattatct tagattgaaa tagcctttca ggtctattcc ctataccgct
     4681 gttccaccca aaccaatctg tcatgaacac gcttcttaaa ttaattattc tcttcttctc
     4741 attcgccttc accctgatct ctcacagcca gcccacccct atcagtaaaa cccccaccgg
     4801 cttcaccgtc gcctgcaaag attacctgac cgaactgcag gtatacagcc ccggcaacat
     4861 ccgggtgaaa aaatatccga aagagaagac attcaacccg cagagtctct ctgttaccgc
     4921 aggcccggtg agcacgccat acagtattaa aacaaagggc acgatggtaa ccctcaaaac
     4981 aacagagttg aatgccatac tggacctgaa taccggtgcg cttactttta cttccctgaa
     5041 tggtacggtg ctcctgcgtg aacagcccga tggtacccgc ttcacctcct ttaatgacgc
     5101 cggtacacca accctgagtg tcaaacaaac ctatctgctg gatacagacg aagcgatcta
     5161 tggactcggg cagcaacaaa aaggaaaaat gatccagcgt aacctcacgc tgaaaatgat
     5221 ccaggacaat actgatgact atgtgccctt tgttctctcc gccaaaggat acgggatctt
     5281 ctgggataat tattcgccca cggtcttcag cgatgatcct tccgccactt ccttcagttc
     5341 tgaagtgggc gaagggatcg actactattt tatcaaagga acaaacgccg atgccgtgat
     5401 tgcggggatg cgtaccctca ccggggcttc gcccatgctc ccgctctgga cctttggttt
     5461 ctggcagagc aaggaacgct acaaaacaca ggatgaaccg gtggaagtag tgcgtcgtta
     5521 tcgtcaatta ggcgtgccga tcgatggcat catccaggac tggcagtact ggagcagtaa
     5581 ttatttgtgg aatgcgatgg aatttctcaa tcctgacttc ggtaacccca aaaagatgat
     5641 cagcgatatt catggcctcc atgcgcacat agccatttcc atctggaact cttttggtcc
     5701 cgagaccaaa caataccgcg aactggattc aataaaagcc ctgatgaact tcggtacctg
     5761 gccgctctcc ggggtggaca aatggccgcc gctgcgggag tatccctccg gtgtaaaagt
     5821 ttacgatccc tataatccgc aggccaggga tatctactgg aaatatctaa gtaaaatgca
     5881 tgccctcggt ctcgatgcct ggtggatgga ttcctccgaa cccgatcatc tggacttcaa
     5941 agacgaagac ctgaacaaca aaacatatct cggttccttc cgcaaagtgc gcaatgcctt
     6001 tccgctgatg accgtaggcg gtgtgtatac acatcagcga caggtggatt cgtccaaacg
     6061 tattttcatt cttacgcgtt ccgcttttgc gggacaacaa cgctatggcg ccaatacctg
     6121 gtcgggcgat gtgacggctt cctggaatgc cctgcgcaac cagatttccg ccggtctcaa
     6181 tttctcactc acgggtattc cgtactggaa tagtgatatc ggcggtttct ttctcggtca
     6241 gttccgcaaa cccctcgcca atgcagatta ccgcgagctc tatacccgct ggttacagtt
     6301 cggtaccttt tgtcctatga tgcgctcaca cggtactgat gccccccggg agatctacca
     6361 gttcggcagc aagggcgaac ccgtgtatga tgccatagaa aagtatatcc gtctccgtta
     6421 ccgcttactg ccctatatct attccacgtc ctggcaagtg acccatgatc actcctccat
     6481 gatgcgggcc ctggtgatgg attttgccaa tgataaaaaa acactcgaca tcaacgatca
     6541 gtttatgttt ggccgctccc tgctggtagc cccggtaacg gaacgtcagt atgcaaaggc
     6601 aacgggcgta aatggcaaag acacgatcta cacgcccgac tattccgtta cccgttcaag
     6661 gcaggtgtac ctgcccgccg gcaccaactg gattgatttc tggaccggca cctctcatac
     6721 tggcggacaa accattgcta aagaaacccc gctgaatata gtaccgctct atgtaaaggc
     6781 cggcagcatc ctgcccatgg gtcctgatgt acaatatgcc accgaaaaga aatgggatta
     6841 cctcgagctc cgcatttatc cgggtgccga tggcgagttt gtattgtatg aagatgagaa
     6901 cgacaactac aattatgaaa gaggcagcta cagtcagatt cgcttttcct ggaatgataa
     6961 aaagaaacaa ctgactatcg ccgatcgtac gggccagttt cccggtatgt tggaaaaacg
     7021 cagtttccgg atggtacttg taaacgaaaa gcaaggagcc ggcgatcagc ctgcgggcgg
     7081 ggatgcacat aaggtggagt atgagggaaa gaagctggtg attagtttgt agaagaatga
     7141 cgatgttctt gtcaccctga gcccgcttcg cccatcgcag ttttaacgaa gctgtacctg
     7201 ttcgttttgt atttttaaaa attcgctttt aatgctgaca gaagaagaaa tactacaaac
     7261 gttggacaat tatcgtaatg gacacgaccc tgcatttgtt caactaggac acccttatgt
     7321 gtttcctatt gactcacgaa ttaatgtatt cagaaacacc cagggaaaat gggcaatagc
     7381 tattgaaaaa ttaggctatg accctcgctc tgaacctata ggacttgaaa ttacttatta
     7441 cggaaattgt ttaattaacc tcgattacag tgaaacctac ttttccaatt cctatactgt
     7501 ttttcccata gatgaagaaa gtttcaaaaa cactattgaa cttgaaaacc taaaaccaga
     7561 tgccggattt tggattgtca gaaataatca aattcaacta ccccataaaa aagaagaata
     7621 tttaaatgcc ggaatagtat taaaggaata tgaaccaaat actatttctg cagaagaagt
     7681 agcacggctt ttagttacga gacaccggga tctgttcaga gccactgacg aagagttata
     7741 taaatctatt ccaaaagact tagataaaat attagttatt gacgaatggc atcacagaga
     7801 tttctatcaa aataatttta accccattga cgacttacct ttcacatttc ccggacccga
     7861 ggagctatgt agagtgatag aagatcaaat tgaaaaatta aatgaactta acattacgaa
     7921 agaaacattg ttaaactctt ttaacctgac agaacaaaat gaaataaggc tcgagcagaa
     7981 taaaaaagaa ttagaaaata acagacctgg gagttatgaa acctggcaat ttatagctaa
     8041 agttattgtg accggtgacc cttcccatta caagccaaca ttagcgccaa cttcacattg
     8101 gaaaaattgg cccgatgcag gctcattgta attaagttta tcaaccagcc ttttttaaca
     8161 gttccttgtg tgccggcaat acattgcagt ccgaacttta gtgacaaacc actgccatgc
     8221 caaaactctc cctgcttttt tggacaatcc tgcttgtcct tgtcattcaa tcccccgcga
     8281 tcgcacagac tcccaacttc accggcacct ggattatgaa taaagaaaaa agcaaaatag
     8341 aagacccgtc tgagggcatg accggaagtg ttttcaggat caaacaagag ggcaacaaat
     8401 tccgcatcaa gatctatcat atctatggtg aaaaagaaaa gaagatcggt ttcaagatga
     8461 aggccgatgg caggactcgt aaggtcaaac tgatttttaa aggaaagctc gagcaaaaag
     8521 agaacagcct gatcgccact atgtggcgga agaacttcct gaatatcgtg aattacaaat
     8581 ttggcgtcaa tgagaacgaa ctcatcgccg acgaagtatt caccggacgt ccccgcgatc
     8641 atcacagtgt gtgggtgttt gaccgggaag tggtgaagta acaggctgtt ttgtcatccc
     8701 gacgaaggag ggatcaaccc agtctgagtc taccacttgc ttaaatcccg accgaaggga
     8761 tggtgagtcc tccgaagctt ttgcgaagga gggatttggc tgttcttgga gatttcgttc
     8821 aatacaggat tcctcctgcc cttcgacaaa ctcagggtaa actttggaac ctgcatatac
     8881 ttctattaca cacatctatc acccctcgct tcgcttcggg gtgacaaggg ttcttagtac
     8941 tcttccgttc taacgacgag cgttactgac cggcaaacac gttggtcgaa ggatgccgaa
     9001 gcataaggta aattttttct taccttcaaa ttatgattct ctcttttata acttataccc
     9061 gtgttgcagg aaaccattga cagatcatac aaagtctttg ctaaatataa gccggcaaga
     9121 ccacttgacg tttgtacgaa ctgctgtatg accccggaag aagaaaatac cctggctacc
     9181 ctgcccttga aagaaattac agctgatttt ttggcaaaat acaacgacag tgccaaaccc
     9241 gccaaaacaa gccttgatga ggtaaagtat tttctgcccc ggtacttcga tttaatcgga
     9301 caattccggt ttccttccca ttcagcggaa ttatcctttt caagactggc tccttttgat
     9361 aaaagcgaat ggacggtgca ggaacaggaa ctcctgaaac aattctccat cgattttttc
     9421 aaccattgcc ttagggtata tccgattccc gcatttaatg aaagtataga ctgtatgctc
     9481 atcatgtttt ggaatagcgg atttgacatc agtaacttat taacctgttg gcaaaatgct
     9541 acaggtaagg aaagtgtatt tcatttccgg gatctttacc tgcatggttt taaccaatat
     9601 aatccggcaa aactttcaaa tgcatttggc gaaaaagaac tggctgatct gctcagaaac
     9661 tggatgcaac aggaggccgt gaaaaaggcc tttgcagaag ccatcgaaat gttgattctt
     9721 gaagaaacgc agctggaaga aaccgatgga tatgaattaa atttactgta tgaccaatta
     9781 cgggcataac aaaatagttt gctgaatgag atggtgtatt ctacctttta tagtttcggc
     9841 tattttaata atatcgctga tctgctcctg cagcaaaacc aacaaaagaa gagtattggt
     9901 tgaagatgtt tatgcagaag gggatatttc aaaagatacc gttttcaacg ggctgatccg
     9961 gttttacgat actacacata accggctggt gatggtggcc agctacaaaa aaggtgtgct
    10021 ggatggtccg cggacagatt attataaaaa tggacgaccg gaaacgaaaa taaaatatga
    10081 aaacggaaaa ataaacggag agttaatcgt ttacgatacg accggaaaga tcactgaaaa
    10141 gcaaaactat tactatgatt tagtaaccgg cccttctcaa agtttcaaaa atggtaaaat
    10201 aagcaactat tcgttttact ccctggacaa tgaaatattg tttacgctcg attatgataa
    10261 accaaaaaag atcgaaaaaa tcaacaacaa cagttatttt ttctggagta taaacaagta
    10321 tacaaccact accagcccgg atccgttaaa cgaacttttt atttacctgc ccaatccacc
    10381 attacttaac ctccgctact cgctctgtat tattgacaag gcttacacgg ttttaaaagt
    10441 ggtaaaggag tttgattcag cattgtattg ggaaaaaaca gacctgaatt ttgccggatt
    10501 gaaaccgaca gaaatgtttg cgttgcgatt aactattgaa aacgcaatgg atgatcctgc
    10561 tgacatagca gttatgttca aaaagctgta aaccatgaaa ttcatactgg ttatatccct
    10621 tttattgttt gcctcctgta aaaaccatat tgatgtagac aatacggaac agcgggaaca
    10681 cggtttatcg tatataaagg gtactaacaa attagtggat ggcaaagtgg tccgcaaatc
    10741 gggtggtaaa atgattgaac tgcagagcta taaagacggg aaaatgattg gcccgttttt
    10801 tcaatatggc cctgacggac aagtggtaac aaagggtttt ggtattgagc tgaaaaccta
    10861 cgagcaggcg atcggaggaa cggacctgac caattgtatt ctttccgtag tccaggtgaa
    10921 taacgtgttt agttatgcaa ctgtttatat ggataatgcc cgtgtatttg atgagaaaga
    10981 aaaattgttg ctgctgacaa agcatatttt agcggactat tcagctactt acaagattga
    11041 cgatttgttt atttttgaca agaaccacga atacaccgtt tcaaaaaacg cggccagtaa
    11101 ggctgattac acggtcgata cagcttcaag ggcaaacccg gtaaaggtga atttcaggta
    11161 gtattatata taattataaa tatttcttcg tgctgaccgg cgggctttca gctgtcgtca
    11221 tagcaccagg acaaacagat agcaataatt ttatgagacc tttctttaca attttatcaa
    11281 ctcttgtttc ggttacttgt tttacacagg tgataactgt tgaatcggta agagtggata
    11341 gtggaatata tgaattcaaa ctacaggcaa aaaaggacgc tgcagactat tcgaaagcct
    11401 ttagaatatt atctaaccag gtaaaacaaa atccattaaa tgccgagtgc cggtattttt
    11461 tagggtacgc aattgaccgg ctcaatgctg aatacggcaa ggacatgatc aatcttaaaa
    11521 aagaaatgac cattaaagcc agcgaacaat ttgaagaggt caatagacta gaaccgactt
    11581 acaaaggcga gatttttatt ctagggccct atgcaaaact ggcttctata tggggaagcc
    11641 tcgcagcctc ctatttaaat agaaaactcg ttgattctgc agtttgggct tttactgaag
    11701 gcaggaaaag gggcggattt atagacccga ttttagaatt taacaggcag cttttaaaca
    11761 gttgcgacaa aaacgccatt cttgttactt acggagacaa tattacagtt ccggcctggt
    11821 acttacagac aattgaaaat taccggacag atattacggt ggtagatgca aacctgattc
    11881 atacagattg gtatccgaaa tttcttaagt ctgagagaaa gctgaaaatc agcttttcag
    11941 atgcggttat tgataccatc gactatatac aatggcaacc tcagcaagtt accatcacca
    12001 atactgttga caccacacag gttttcagtt gggaattacg gccaacctat ttggacaaat
    12061 acatacttaa aggagacagg attctactgg atatttttca acaaaatttc ttcagcagac
    12121 ccatgtattt ttacaatgaa tccgattctt ctttcaatct atttctaacg ccttatttgg
    12181 ctgacgaagg attggttagc cgggtaatga caaagttgat tgattataaa aaggacgtaa
    12241 ttactgtgtc aaaaaacctt tacgactaca atatcgacaa gctgaaaaaa gaagatataa
    12301 ctaagtcaga tgatgcggtg agtgttttaa attcctttcg ctgggcttac ttcaacaaca
    12361 tatatcattt attgacacag gcaaaatatg acaaggcaaa ggaattgatt aaactaatgg
    12421 ataataagtt caaaaaagac aaattgcctt ttacatctgt tgaagttgaa aaatatttct
    12481 ctgaactttt taagcgggtt gacaaagatt accggtaaaa tttagtgagc tgatatgctt
    12541 gctgaattcc gtcaactaat tttagtacgg gacaattcct ccaatcgtcg gaatctatat
    12601 gcacgtccac gatcaatgcc accacccctc gctgcgcttc ggggtgacaa gggttctttg
    12661 gagataccct ttgctacgat aaaataacca gactgacata cacctaccct atacctcatc
    12721 cgaaccatct ctaaactaaa agcgcatcat gcgctttatc cctatccaag ccccgaacaa
    12781 aaccaatacc aggtctggaa aaaactcggc tcagccccac ataaatcatt gcaactcttt
    12841 gccttacaac taacccacgc tcacttttaa cagtcaacac tctctaaccc tctctttttc
    12901 aacccattgc cttaactgaa tctggtttcc ttcgcaaatc cttcgtgttt tcattgacaa
    12961 tacctgtaga ttttgcgaag gagagtcgaa caagtcccga agaaaggtcg aagatgagta
    13021 aagtcgctaa ggggtgtcct gtactgaaaa tgccccagtc ttgaaaccca aaacagctaa
    13081 gaaaacctcc ttgttaacct ttcaacccct caacaatcaa ctacccggag cccacaataa
    13141 ccaccccgcc cctcccgttt cacagcaacc ccctgcccgc caacaccatt aaacgcttat
    13201 catgaaaata ctgctattgc ttttattgcc cggcttgttt gcggatatac atggacagca
    13261 ggccacggca aagaaaaatc cggttacagt ctatctgggt gccaaaacaa gagtcacccc
    13321 catctattta aaacggctgc atgaaggttt tgaaatagga ttccccaata acatggagca
    13381 acccgattac catttatccg gaccaggttt actgattagt gaaaagaaag ccctgggccg
    13441 taaactggaa ttttccctgc atcaggtgat ccgttatgat tttctttatc aacgaatgcc
    13501 tatggacctg gttttaccgc ccgacttcag ttacagtata aagcggaagt ggatcataga
    13561 tcttgaggct gagctgagcc accgtttccc gcgcaggaaa tcggtactcc tcgtatccct
    13621 gggtgccgga ttgaatggcc tcaacaccgg tttccaggaa acccgccgct tttaccaatc
    13681 ccctaccagc tacactgaaa aagaaacccg taaaaacttt ctgttcccct ttctcagtac
    13741 cggctttggc tggcaaaaga aaaagctgtt ggcgggaatg aaactcggct attgctggga
    13801 caaccccacc gtttttgcca gccctttttt gtatccggag ctgagtgtgg ggtatcggtt
    13861 gtttaacgga aaataagagc atgcatataa aacgaaaacc tgttagattt actttgtaat
    13921 aacaaaagct gattcttaaa tgcctggctt tacgctcatg gcgaaacgct gcatactata
    13981 tgggactaga aactcacccc tcctggtacc gcgacctccc cacacttcgc acgctcatcc
    14041 ttggcagctt cccgccccac ccatccaaac ggatctatcc ctttttctac cccaatggcc
    14101 gcaaccggtt ctggaagata ttggccgata tagcaggtat gccgctggag tggaccagga
    14161 aagatccggt caaagccgtg gaggaacgct atgcgattat gcaaaaactg gaaaccgggg
    14221 tacagaatat cggctacgaa attgaacgaa agggcacaag tgcactggat accgatatca
    14281 ggattaccaa ataccacgat atactctcca taattacaac ccatccggaa ctgacaagga
    14341 tcctgttgcc gggctattca gcggaacata gtaccacccg gtcgttttta aagtacatcg
    14401 aacaaaaagg aatacgttca tcggatattc cgcccatcaa agctgaagcg gcatttaaaa
    14461 tatatgttaa tgaccgtgaa attgagtgtg tcattttaaa ctctacatcg acggcggcaa
    14521 agatcaggta tgaagatttg ctggagcagt ttaagcacta tttgccctaa ctttagctat
    14581 ataaattgaa ctcgggaaat gccggcttcc cgaaaaccac ggatgttcag acttctattc
    14641 atacttacct tatccctcct cctcttctcc acctgccatg taggagctga taatacaatg
    14701 ctgaaaaagc cagggtctat tgatagtctc cataacgcaa aacaaatcac ggctttcatt
    14761 caccagatag atagcaacta tagaaaattc atactaaatg atccggtaaa ctatgaaagc
    14821 cggtatgcag cattcaacta taaaagagtc gccgatagtt taggtgtccg gccatggtct
    14881 aaagcggact ttgaccataa cggattaact gatctcctgg ttatcgggca gcacaatgac
    14941 cccagtatcc tgatcatcct gaacaaaggt gaccattatc agatcatacc gatcacccgt
    15001 cgctctttcc aggatcacac attcccggtt gttgaaaaca ataagatcag atatactttt
    15061 gaaaccaatt cagacaagat ttaccaggta ttacctgcca gcctggaaac cataaccctt
    15121 gtctataaat tcggtgattt tattgaagag aatccgttgc cggctgaaca caaaattgaa
    15181 aaaattgaat acaatactac ggggtgcttt ggcacctgcc cggttttcaa gatgacaatc
    15241 aggactgaca ggtcatctgt atgggaggcc attcgttata accaatttgg ttcgcataaa
    15301 tcagccggaa catattcctg tacaatcaca gaagacaaat atgcagaact gacgaacctg
    15361 ttaaactata tagactttga aaagctgcaa cttgattacg ccgtcagatg gaccgatgac
    15421 cagcaagcaa ccttaacaat cacatttgat aatggccaaa gtaaaacgat ccttgactat
    15481 ggcctacttg gcacatacgg actcaaccgt gtacaccagc tgatgtttaa tttaagagaa
    15541 aatcaggatt ggaaataaat cgaaaactaa tgccactgaa aacctattta ccccgtcatt
    15601 ttaattttcc ctggtttgtt ccgattgcgc tttgctgttg ccttatcctt ttgagttgca
    15661 aacaaaataa gaacccctgc aatctccctg catctgtcct ggcagatata gccaaactcg
    15721 attccctcgt caatgttccg gcactaagag aacgtgatag tatatggaat gccggtggtt
    15781 ataaagaagc ccctgtatat tcagcccaaa acgaaacata ccgttttatc tggaatagtg
    15841 cttttaatgg atcaaaagtt tgccggattg aacatatttc tgactcctat aacgtaacgg
    15901 taaaaaaatt tacaaaagat ggtgatatag cagagatccg tcattttagt attagtaaga
    15961 aatcctggaa caatataacc gattcgctgg ccatccttgg attctggact tatttccacc
    16021 cgccatataa aatggtattc gacccagaca actggagact ggaagcttac aaaccaatac
    16081 ccgatgcctg tacgggtaaa cgctatcaca gtgtatcggg ctcttcacca attgataaag
    16141 gtctttacaa catgtgtaag ctgcttaacc ggttagatga gtcgctcgtt gagtagtttt
    16201 tttgcatgtt tgatttaaat ttgtcacctt atagatacct tcattcacta accgaaaccc
    16261 ttccatgttc tacctctacc gtcgcaacat catctccttt ggccacaacg atggcgatga
    16321 aataacagat aagcatacca tcctgctgga ccatgccagc tcggccagta tgattcagtc
    16381 cctacgggat aaataccagc atgcatttta taaacagctc tggtgttata aagatttgac
    16441 agctgaagaa aggcaagtgg tgctggaagt actcggcagc aaggtaacgg aggacaactg
    16501 ggaatcagaa ctggagcgga tttccgttgc cgaatacaaa aaactggaag ccaccctgca
    16561 attcaatttt acaaagatca tcgaaggcaa tccgcagcaa gaccggtttt acccgctgat
    16621 caaacaccag caattcagtg aattcgggct ggacagcaaa tattatcacg acatggatca
    16681 cctgcccccg ctgctgatca gcaacaacaa acaggaagcc ttcgagaaag gggtgctata
    16741 cctgttgctg accaatgatg tgagccatta tatcggcgac ccggaaacca ctccctacga
    16801 ggctgacttc cgggaacgct ttgccgcacc ccggaaaaag atgctggact atttcagtaa
    16861 atccgccttc ccctttctca aaaaatcggt atcggccgaa ctgatctcca gacaccaggt
    16921 gctgaaattc tggattctcg agaatcccta cttcattaat acagcacagt ttgaacaatt
    16981 cctgaagtac aaagaagaaa tcctgaaaga caaactggac cattttaacc ggatcgtgga
    17041 gttgttcatg gaaaagaaga atgaattgat cgaagtgatc aatatatact gaactttatc
    17101 acgtcaaatg acgaacgaag tacactatac caaaggcgat gctactcaac cgaccggtac
    17161 gggaaacaaa gtaattgtac atatatgcaa tgatattggc ggttggggca ggggtttcgt
    17221 tatggcaatc tccagccgct ggaaaaaacc tgaaaacgaa tacagaaact ggtttaattc
    17281 agcggtcaat ttttcactgg gcgaagtaca gtttgtacaa gtagaagtag aaacctgggt
    17341 ggcaaacctg atcggccaac atggcatcaa aagaaacaat caaaatattc cacccatccg
    17401 gtataaagca gtggcagatg gattacgaaa agttgctgat ttctgtaaac cgcttaatgc
    17461 ctcggttcat atgccaaaaa ttggttgtgg cctggccggg ggcacctggg atgaggttga
    17521 acccataatt atctccgcct ttatacctta cggaattccg gttaccgtgt ataattttga
    17581 ttaaactaat ttttaaacat cctgcaaaaa ccaatctgat cagttattcc aacctgattc
    17641 cctacaatca taaaaaacca aattaagtag atttacatcc aatcatttac taatgtcttc
    17701 ctccaaccac ttctccgact actacaaaac catcaccgct acagaactgc tggccatact
    17761 gaataatccc ggtgactatc agccgtcggc catcgaagcg gccaaggcgg aactggagag
    17821 ccggcagctg agtgagacag cattacagga agcgcggcaa cccctggtgg agaaagtgca
    17881 gcggaaagaa gcggagcgga agaaaataaa agccgtggaa gaaaaattga aaatcgccgg
    17941 gaccggcttc tttgaggcca tcaacccgat acaacaggga attccttcta cggagaaaat
    18001 gatcaggcgg ataacaattg tttttggagt gatttttctc taccagttaa taagcgattt
    18061 cagggaacat attgcctata tcaaagactt ttccagctac ccctttctta ccgttttata
    18121 ctttttttcc cttgtgctgt tacccgttgc attattattt ttctggaaaa gaaaaatgtt
    18181 gggctggata ctcttaacca tttacctgag ttttactgtg gtaactgttc tttggtttat
    18241 gttcctgact tttacccgta aacattcagc ttatggagtc tttgaaaatt tttatcagac
    18301 tccaccctta actgtgttta ttattcagct gttgtttttt gttgcaacca tgtatttaat
    18361 gtgccgaaaa aatatccggg aagtatattc aatcaccgat cagaaaatga tggcgacaat
    18421 aagcattacc gccctgataa gtttcttttt ggcaaatgca gctaattagg gtgaaataag
    18481 aaaccggcaa atattttccg gcaccgattc gcattttgac ttaatttgtt ccggcatatt
    18541 caaacctctt ccataattat gaaatcagct gcaccctgaa attttcggaa tgcatcacct
    18601 ggttttattt tccacccatt aactttatcc tctaaattgt aaagtatgac tcccattgaa
    18661 acagccatta aaatgaccct ggaccgctgg tacggttctg tagccagcct ggataaggtg
    18721 ctgaacgtac tgacagacga tcaattgcta aaagaaacag ccccgggcaa aaacaggggc
    18781 atctatttac tgggacacct ggtcgccgta cacgatgata tgctgaccct cctggacctg
    18841 ggtgaaaaac aacatccgga gctgactgag ccctttataa aaatggccga taaagtgacc
    18901 acggaacttc cctccgttgc tgaactgcgc gactactggt ccggacaaag ggccttgcac
    18961 cgggaaaaat ttgatcagtt tacagcagat caatggtttg aaaaacatac ggccgtttca
    19021 gcagatgatt ttgccaaaga accgcatcgc aacaaattaa acataatcat ctcccgtact
    19081 tctcacctgc aataccatac cggtcagatc gcattgctta aataaccccc catgagtaat
    19141 caaaattttc aatacagctt taccaccccc aaaaatccag ccgaagtatt tacgcttttg
    19201 ctggatccca aaaactggtg ggtcggtttg tttggtgaaa ccattgaagg aaaaagtcag
    19261 gcggtcaatg acgagtttac attcagcgcc ggcgatgggg tacatttttc caggcaaaaa
    19321 ttaacggagc tggtacctga taaaagaata gcctgggagg taaccgaaag caatttatca
    19381 ttcctgaaaa acaccaacga atgggccggc acaaaaatct gctttgatat agaaaatgaa
    19441 aacgggaaaa caaaagtcct gttcacacat gagggactgg ttccgcattt tgaatgttat
    19501 gggggatgct cgggagcctg gacacagtat atggagaagc tggagaagaa gctagaagct
    19561 agaagctaga aacgagaagg aaacagcccc tgctgagttg gtttgtatga agaatgtact
    19621 gctactgcta tttcgagcga agcgagaaat cttgtatttc ctttctctct cccccacagc
    19681 ccgtcattcc gaacaaagtg aggaaccttg tatttgcaag gcagcgagaa atcaaaacat
    19741 cctctaactt cccgatgcat cggtctgtaa aaagtatctt tggtttaaat actacttact
    19801 tatgccaacc actatactcc atcccacttt catactgcga ctggccgttg ccattattct
    19861 gttcatgcac agcattcccg gtatgtttaa taacgggatt aatgattttg gcaatcacta
    19921 tcttaatgag gttggcttcg cccctgccgg ggtacccctg gcctgggcca ttaaactttc
    19981 ccatgtagcc gctgccatct gcctcttgct gaacaaatat gtaaaatggg ccgctgttat
    20041 caccatcctg atcctggtag ccggtattgt gatggttcat tttaaagaag gctggtttgt
    20101 ggtaggcggg ggaagcaatg gagtggagtt caatttcctg ctgatcgcag tattattaac
    20161 tttcttgttc ccgaatgagc gtaaaacaag ttaaacttcc tcatagaact aacgaccaac
    20221 ccattagaca aacaatcact ccttcacctt aatcaccact ttcccctttg cccggccggc
    20281 ctccacataa gccagtgctt catttgtttg gtcaaaagga aacactttat caatcaccgg
    20341 tctgatgaca ccggcttcga ttagtttggt gatctgactc aattgcgctc catccgcccg
    20401 cataaacagg aaactgaatg caatatttcc ctttttggct tttcgcttca tacccgaact
    20461 taatagactt gtaagtagtc ttaaatacca gggcaaccca atagctgttg caaattcagg
    20521 ggtgggcgga cctaccaggg agatgagctg accgccgggt tttaaaatct gtaacgattt
    20581 atccagcgaa cccttttcct tactactgtg caaaaccatg tcatatcctt gcagtatagt
    20641 ttcgtaccct tcctttttgt aatcaataac aaggtcagca ccgaggcctt ttactaaatc
    20701 catgttcttt gcacttgtgg tggtagccac aaaagctcca acatgttttg ccagctgaat
    20761 ggcgaatatt cccaccccgc cggaaccggc ctgaataaac agtttttgcc ctttttccag
    20821 tctgccaatt ccgataagtg cctgccaggc tgttagacct accaacggaa tggaggcggc
    20881 ctcttccata gagaggttag ttggctttaa tgccagtgca ttttcatgaa cagcaatgta
    20941 ttcggcgaaa ctaccgatat gatgatccgg tacccgggca tatacttcat cgccagcttt
    21001 gaaacggctc acctttgatc ctgtgctgat caccacaccc gccatatcat ggccgtttac
    21061 aaatgcaggc ttatagggca agaacaattt gaattctcca ttccggatga gtgcatccag
    21121 cgaattcact ccggccgaat aaatttgtac caatacttca ttttccttta ccgctggttc
    21181 tgcccaatcc gataaatgta atttttcttt tttgccgtat ttttttacga tatatgcttt
    21241 catgatactg tttatgattt gttctgctta tgaaatggag aaatactttt tttaaaaccc
    21301 cgggattact aactattcaa ttgtttcaat acatttttct ccaaaaaccc atacgcaaaa
    21361 tgctgtaagt gaattaatgc agaagtaccc ttccccacga tatttctgaa ttttggatta
    21421 tccgtttcca ctactttaat cactttacca gctacgatgg ctggatctcc ggcttgcgca
    21481 accaggttat tggtgaaccc ttcaattttc cgtctgagca gatcataatc gtcaattttc
    21541 agtactgcag tggatgaatt atcgaggata ttggttttaa aggccgccgg ctgaatcatg
    21601 gcaacattga tattaaattc cttcagctca taacgcagtg atttgaaata gccttccaat
    21661 gcatgcttgg aagctgcata ataagcacca ttcggaaagg ataccagtcc gacaatggaa
    21721 cccaccgtta tgatcttacc ggatttttgt cttctgaatt gtggtaataa tgcgttagtc
    21781 acttttatag ttccccaaaa attggtttcc agttgttgcc gacccagctc aataggcgtt
    21841 tcttctgcaa tgcccgatac taaaaaaccg gcattattga tcagtacatc cagtttttta
    21901 acttcactga aaacgctctg ggccaaactg ttgatggatt cagcggaatc cagattcagg
    21961 gatatcagct tgaaaggcag ctttgatgca tacttttccg ggtttcggct ggtgccaatg
    22021 acatggtgtc cccgcttgtg cagttcggta gccaccagta atccaaaacc ggaagaagca
    22081 ccggttacta atatgttttg tttcatgttt ttagtttaaa aggtgtatat ttgcttgcat
    22141 tttgcaagtg caaatataga attaactttc ctattgcaag tgctttttaa aactttttta
    22201 tggaaacaag caaaaaaagg tcggattgcc ccataagtgg ctcattagat gtgtttggtg
    22261 ataagtggtc cttactgatt atccgcgacc tgatgttcgg aaataagtgc acgtacaacg
    22321 actttttaaa atcggatgag ggtatcgcca ccaatatact agcttccaga ctgaaaggtc
    22381 tggaagagca tggcattatt gaaaaatcag cccatcctga cagtaaagca aaatttttgt
    22441 accgtctgac ttctaaagga attgacttat tgcctgtttt aatggagata tacatctggt
    22501 ttgataaaca ttatgaaatg cccgccgacc ttaaagcaac catgagggat gcaaagaaag
    22561 acaaggaaaa gtttgcaaaa cagatcataa aagagttgag aacgagaaag tcagataata
    22621 aaagcagcag ctgaataaga gaattcgatg atatcagcat ttcacatatt gcctgttccg
    22681 ttacgatgcg gtcgccaccg gtaaaacaat tcccctcttt tttcaccgga gttggataaa
    22741 ttcaccttat caattatgaa aaagctgatt cattgctttg ctctcctttt cgtgtttagc
    22801 ccttttctgc acctgcgggc tcaacccccc ctcatccaaa acgacacctt ctggtttacc
    22861 aaagaaggcc agcctatcta cagccagggc ggcggcatct tccgttttaa agatccttcg
    22921 agtggagtag aaaaatatta ctggtacgga gtgcgttacc gcgaagcaga aacctaccgg
    22981 ctgaatcctg cagtcacctt acctacgtct acttttgaaa gcgtgacctg ctatagttcc
    23041 atcgacctgg tgaactggag ctttgaacat aatatactca ccatggccga actggaaaag
    23101 aacggcggtt ataaacgctg gctcggacgg ttaggtgttg cctggctggc tgaactgaaa
    23161 aaatatgtga tggtggtgca acacggcaac caggtactat ttaccacatc agatacacct
    23221 gcgggtaatt ttacctggca caaccagatc agcatgaagg aaatgatcgg cacccccaat
    23281 accggtgacc agacggtatt cacggatgat gatggcaaat cttacctcat ctattcatat
    23341 ggcagcggac ggaataagat ctatgtatcc gagatcggtg taaaagaagg taaagctaac
    23401 ttgttggact gcaaagaaat tttccgcggt gaaagtcgcg aaggcaattg tatgttcaaa
    23461 tacaatggca aatactatat ggccgcttcc aatatttatg gctgggatgc ctccctcgcc
    23521 tactacctcg tggcagatga tatccgcggt ccatacactc cggcaaaaag tatggcggta
    23581 atgaagggct gcgaagacga ctatgcccat atcactcaaa ctggtttctt tgtttccgtg
    23641 aaaggcacga agcaggaaac cattctatat tgcggtgacc gctgggccga ttttgcaggc
    23701 aatggacttg gttataacca atggttccca atgtcatttg aaggcagcac gccggtattc
    23761 aattccctgc accgctggaa cctcaatgca caaacaggct tatggtccgt agacccaggt
    23821 aacaactatg tccgcaatgc gagttttgaa gccgaccgcc gctatgttcc cacccacgta
    23881 aaacccgtac aggaccagct caccggctgg accagcactg tcatcctcgg caatgccatc
    23941 agccaggatt cactcacgag tccggtactc aactatttca acaccgaatc cgatcgcaaa
    24001 accgtaattg gtgaaaaaag tctagccatt accgataaga taaaattcaa tcgtaaggtc
    24061 tcgcaaacta ttatatccac tccttatgta aaactgcaag acggttacta ccgcctcagc
    24121 gcgatgatcc gtaacagcgg ttcattcgat acgatgatga tgtatgccaa aagcaagggc
    24181 aagacattcc gctataccat caccggtgaa aacaatgaat ggaaaaagat caatctcccc
    24241 catatcaagg taagaaaagg aaaagtggag atcggatttg aagcagcggg tgccgccggc
    24301 gcggtatggc tggtggatga tgtggagtta gtgagggagt gaggggcttc ccatttcggg
    24361 cgccgcgcct ccgtttcact actaagggca tggagcgggt aatacggtta gctgccttcg
    24421 aacaacgata atcaggaatt taccttcctt ttttcccgaa ctttacagtt aacgaccgat
    24481 tctcttcttc aaatagtcct cccatgcctg ccacttcctc caagcaactg caaattgcat
    24541 tagctgtcct gctgttcttt cctatagttg gcatcagcca ggtgaaacca cagatccgca
    24601 ccgggtttga tgcgatgtat catattgaac aactgccatt gctctttcca aatggcacca
    24661 gcacccgtca gcccctcgct tatgatccca cgggtcagaa ctgggaccat cattttatgg
    24721 cggcttttac ccaatatgtg gactccatca aacaggatga cggaacaatg gtaaaagaat
    24781 atgtgatcta tgatgaatac ggtcctggtg tattgtaccg ccaacagatg aatgcctggt
    24841 acgaccgttc caaaattccc aatggctggc tggcctgggg agaagccgat cagcccaggg
    24901 ccaatgcgaa tatccggtac tacttcgatg acgaaaaaga accccggatc aatatgcacc
    24961 tgaaggattt ctttggtacc caaaaatttc cctttactga accactggct tttgtggatt
    25021 cgggctactt gtttgccaac ctgtattacc cctttaccta taaaaaaaga ctgaaagtaa
    25081 ccatgcgccc caatgcaaaa tcatttgagc aaatggatac caagtggtac cagtataccg
    25141 ccctctcctt cccggcggac tacaaactgg aaagctggac cgggcaatcc gcagttgcca
    25201 acccggtaat agaccaatgg cgaaacaccg gcgataaccc caacaacctg gaagacagta
    25261 aaaaaattac cagtcagaca agaattaaaa aaggcagttc cgcaacgatt ttatcgatca
    25321 atcaacccgg atcccttgcc gggtttaccc tgcaactgac cccttataac aaagaaacgc
    25381 tattcaatac cacattgaag atctactggg atgatagcaa agtgccttcg attgaattac
    25441 cactcggtta ttttttcggc gccggggcca aggactatcc cgctacggtt gataaagtgg
    25501 tacagaaatc acttactacc ttactctttg gttttaatcc gcaaacagga acactctatt
    25561 cctattggcc catgcctttc tggaaatctg cccggatcgt aattgccaat aacacggata
    25621 ccgatatcag caacctgcaa tgcactacct ggtttaaacc tgctgctata ctctcctaca
    25681 ataaaaataa tacaggttat ttcctggccc ggcgaaccat tgactccgat ccggataccc
    25741 tgggctaccg ctcagtggct tttgaagaaa aaggaagagg tcatgtaacc ggggtggtct
    25801 tttactccga ccgctatgat atggatggtg atgaattttg ctatattgac gatagtaaaa
    25861 caccccagat acacggcagc ggcacagaag atgaccataa ccagggatgg gccggaagag
    25921 cttaccagca accactctgg ggcggattgg taaacgggta tgacggcgct taccggattt
    25981 acctcaatga ctgctatatc ttcaaccgga atattcagat cacttatgaa tactccctta
    26041 caaaacccca ttttgtaaac gggggaaaaa cagatgtaat tgtgtactat tataaatcag
    26101 ccacccccgg taacctgcaa ctcaccgatg aactggatcc cggcaatcat tttagtgaac
    26161 aacagcatca gtatgaagta accggactta cctggaaagg aaaactgaag gatgaatacg
    26221 atggctatga aagaaacctg ctgtatggcg cttataccga cgatggtaaa gcttataacg
    26281 ggagcagccg gttcaccgtt caggttcatc cttccaatga aggcgtaaaa ctgcgccgga
    26341 gaatcaaccg caccggcaac ggggtgcaga ccgcccttgt atatattgat ggaaaacagg
    26401 taccccgtcc ctggcatatt gtaattccat ccctcagtac cggcaaaggt ccggtggatg
    26461 gctggtatga cagcgatttt gaaattccgt catcctttac caaaggaaaa aaatcgattg
    26521 aagtaaaggt tgaatatgtc aactcaccac aaaaaggcgc aatcaacgaa ttttattact
    26581 gggtatatag ttatgtgggg ttgtaggaat cagatgtcag ttgccggatg tcagttgcca
    26641 gtaaacagcc attccgaaac aaagtattat aaacctgata aacatcccgg ctgcacctca
    26701 acatcgtatc tcctgtttgt atatttgttc tttcggggac ttccgtggac cgttgtctgt
    26761 cgactgtgga caataccctc ccagcccatt caatcaacca atatgaaatc aaccttattc
    26821 acgcttttta ccctggtctg tctgcaggca ttggcccagc ccgttaccga actcagggaa
    26881 acggaagtat acttcagacc cacccttact atcccgggca aattgcgggt gatgtattca
    26941 gctgacagca gcctgctccc ggccggtcac tataaaacaa gcggcggcaa ccggtaccac
    27001 ctgttccagg tgggtgactc cggatacctg aacggctcct ttaaagattt tacagatcag
    27061 caggtttacc gggaggtaac ttatttaaac ggtctcgtaa accgggaata ctttatcatg
    27121 aacgggacag tacacacgga ggcattcgac tccactgtta ccgtactcct gtataatccg
    27181 aaaaaaaata aatggaatcc ggaattaaag tcagtcaggg tggaaaaaga atatggtcag
    27241 aaagggcgga gactgatcat cagttttaca aaagggccct ataacgctta tgcccgttat
    27301 aaatacgtgg atggcatcct tgtcagtgaa atgatccccc attatcatga aaagaaaatg
    27361 gatccatccg gaaaactata ccagctcatc caatacaact ggaccctgaa gcagatcgaa
    27421 accagtgaat tcgaaaaagg catccttaaa agcaaatcca ttctgaaaaa cttctcgatc
    27481 gtctgggacc cgaaggggat cgttcacttc acagatgaca ccgggcgctt taatgatata
    27541 gtgaccttca cttactatga cgatcaaaag atcaggaaaa aggaagtgga gaaaaacggt
    27601 aagcggacgg taactgaata tgacaaaaaa ggtaaaatca cttctgtgaa tacctacccc
    27661 ctgccaacct cagaatcgga aatatttgag gtagcgccgg ttggggaaaa acaataaata
    27721 caaagtacaa ggtaagaagt acgtgcggga tgccgtaaag cgagtagcag tacgttgtat
    27781 ttgataaaat cttctcagaa ggggctgttt cccgacaccc gacaactgac aaccgttcac
    27841 ccctgtatat ttcagtaccg gcaaataccc attaccagcg gcattgctat tcagcattcc
    27901 cggggcatct ttgatgctca atcctgttta tgctgaaatc ttgctggctt tttatatcat
    27961 tactcggggt cgcgttgagc gcaaaagcac aactggcaaa aaccgactcc ctctacctgg
    28021 ccctgaaaca acaggacagt atcttcttcg aacggtcctt taacttatgc gacctggact
    28081 atctcgataa aaccacgcac ccggaattgg tctttttcca tgaccaggga ggcatacagc
    28141 acaaaacaga gtttctcgcc ggagtaaaaa ataatatctg cggaaaccct gaacaaaagc
    28201 ccatccgcaa agctgtggct aacagtcttg aagtatttcc gctttttagc aatggaattt
    28261 tctatggcgt gatccagacc ggcatccatg aattttatat ccgggaaacc ggtaaacccg
    28321 accgctatag cagccgggca aaattcaccc atgtctggct gctcgataat ggccgctggt
    28381 tgctccgcga agtactgagc tatgatcaca agtagaagtt taccggcact tggatacttt
    28441 tttctctccc acacagcttg tccttccgac gaaggtggaa acttcgcaca gggacgtggg
    28501 ttgattttca ttgaaaaaca aatgcctgtc aggttttatt cgcttaggag gggttgttta
    28561 cttccgacta ctaacttcta actaccgact tccttttctc cctaccttta tccaatgcct
    28621 ttcagctaca acttcggtga atttcccgga cagactatta accaggggcc caaactgatc
    28681 agcttcagcg agtacaacgg tgaagcggat attgaactga tgtgtacaca actggataaa
    28741 aagaccgcaa aagaacagtc caggatcgtg gatgaatggt gtgatcacct gacgaatgat
    28801 accctgccat tgaaaagcat ctggatggaa acaaggatct cgcagaaaat cttcgatgcg
    28861 atctgcaaac agaaaagtct tacttccctc tggatcaaat ggggggtata ccctgacctg
    28921 tcggccatcc gccaactgac caacctcgaa cacctgcacc ttggtaatgg aaaaagtatc
    28981 acggacctct ccccggtggg cagtttgcat caactgaaaa cgctggaatc cgacggattg
    29041 gtaaatgtgc aagactaccg tttcctctct tccctgaccg ccctgcagga tctcgatatt
    29101 gaaggagatg ggttct
//
LOCUS       DAOPMW010000007        28473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1437_length_28473_cov_8.590928, whole genome shotgun sequence.
ACCESSION   DAOPMW010000007 DAOPMW010000000
VERSION     DAOPMW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28473
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_28473_cov_8.590928"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..863)
                     /locus_tag="PLU11_00830"
     CDS             complement(27..863)
                     /locus_tag="PLU11_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57676.1"
                     /translation="MKKSIQFCLSLLLLSVFNVSVFAQKTTTYAQSTTQLQLEKNNIQ
                     VENLSFTDALGPENQLQHTASFDFFLSDDAATQNILRILQVYTKEQASRQLNFVTLNY
                     AGDPLQERFYDNAVVEEILFNALDATSKAPFKATVKIRAGSVKIQKGGTRPDLAPRNK
                     RRALTANFSITLGNLPTTRVAKISGLGIKPGDGSYINLTIEVMAIDGAAWNQWFLTGA
                     AGLRKEKGNIHLLAPDLKGVLYSFSLYDVEIVSYTGGSSGNQQTISRATIGLRLKGLA
                     VK"
     gene            complement(985..1503)
                     /locus_tag="PLU11_00835"
     CDS             complement(985..1503)
                     /locus_tag="PLU11_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPN57677.1"
                     /translation="MSVLKPECPQFFTATNLWWKPLLKDDAHKEIVISSLRYLVEKRR
                     ISMHAFVLMSNHIHLIWKIKPDHRPENVQRDFLKFTGQQIKFSMQKTNPGFLELFRVD
                     SKDRKYQLWERNPYSQALWTAPVYLQKLQYIHDNPVKKGFCKDPEDYHYSSALFYETG
                     KDNWGFLEKYDI"
     gene            complement(1879..4641)
                     /gene="ppdK"
                     /locus_tag="PLU11_00840"
     CDS             complement(1879..4641)
                     /gene="ppdK"
                     /locus_tag="PLU11_00840"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPN57678.1"
                     /translation="MATKTKTGKYVYFFGGGKADGNESMKNLLGGKGANLAEMAGHPN
                     LKLPVPPGFTVTTEVCTYYYANKRTYPKSLNAQVKESMERMEKITGKKFGDEKNPLLL
                     SVRSGARKSMPGMMETVLNVGLNENTIKGVIAQSGDARFAYDAYRRLIMMYADVVMEK
                     AAGIEPKGGKGIRKVLDEKLEHVKKTRGYASDTDLTAEELKELVKDYKKTVKKVLGTE
                     FPDDPWEQMWGGVGAVFASWNGKRAIEYRRIEKIPHEWGTAVNVQAMVFGNMGEGCCT
                     GVAFTRNPGNGENHFYGEYLINAQGEDVVAGIRTPSPVNDYSKNAQSEHFSTLEELMP
                     AQYKELYTIQRRLEKHYHDMQDIEFTIEKGKLYMLQCRVGKRNGVAAVRMATEMVKER
                     LIDAKTALMRVGPNQLVELLLPMLDPKAELLAPVIAKGLPAGPGGAKGRVVFSSAEAV
                     EWASKGEKVILVREETSPEDVDGMHKAQAILTTKGGMTSHAALVARGWGKCCIVGCGD
                     IEIHAEAKNFHAKNGVVIKEGDWISLNGTKGAVYEGSMALVDIDIDKNQSYKDLMKLV
                     DKFKQIGVRANAETPLDAVNAVKFGAEGIGLFRTEHMFYGEGSEEPLFQLRKMIVSKT
                     EKERREALNGLFKFVKKDVKNTLSVMKGHPVTIRLLDPPLHEFVPHDKEKLRQLSKEL
                     GISMGMLNRRVLALHENNPMLGHRGVRLGISYPEITEMQVRAIFEATAELLKAGKKAF
                     PEIMVPVTVIAHELRHQKVIVDQVYKEVCEKFKMKKIPYLYGTMIETPRAALRAEFMA
                     NVADFFSFGTNDLTQMSFGFSRDDIGGFLPKYLDLKILPDDPFQTIDQEGIGQLIKVG
                     TERGRVTKPNLKVGICGEHGGDPESVKFCHRIGMNYVSCSPFRVPIARLAAAQAAIET
                     PRKQKK"
     gene            complement(4798..5187)
                     /locus_tag="PLU11_00845"
     CDS             complement(4798..5187)
                     /locus_tag="PLU11_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57679.1"
                     /translation="MAINKNHEFEELDGVKCGIVEKNVSPERLAFLKPLLELNGFTVV
                     SVPSPAPKAAPPPKVAEGETAPPPPPPPTPPSTFTVGVTDYTFNATNAIFGRMLKTKN
                     GHVVTHAYWLQKEAESHEEVPYYEFKG"
     gene            complement(5197..6594)
                     /locus_tag="PLU11_00850"
     CDS             complement(5197..6594)
                     /locus_tag="PLU11_00850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPN57680.1"
                     /translation="MNELFTLMKSELVLTGIIFLLLFIKIGKGVKNETLLPLAQVLLL
                     ANFVFGFVLNTEGRLFGDMFYTTHLIAFQKNILNLGVYLITLLCADWLRKTPHLAEFL
                     LLMLSALLGMFFMISSANLLIFYLALELATIPVAAMANFDLDKKKSAEGAMKMILSSA
                     FSSGILLFGISLIYGSTGSISLPEIQERLDGSTLQILAFIFFFTAFAFKLSMVPFHLW
                     TADVYEGSPIAVTSFLSVISKGAVAFIFMTMLFRVFTPMKEVWQVMIIILSVLTMVVG
                     NLFALRQQNIKRFLAFSSIAQVGFILLALSSDDFHMGSTAVIYFVLIYLFSNLAAFGV
                     ASVITERTGKENIDDYKGLYKTNPFMSWILALALFSLAGIPPTAGFFGKLFLITAGAS
                     GAAYWFILVAALNMIISLYYYLRVIRAVFMDKNDNPIEPLKLMAPVKLGMLLCGAGIV
                     LAGLLSWIYDYIHSL"
     gene            complement(6606..8084)
                     /locus_tag="PLU11_00855"
     CDS             complement(6606..8084)
                     /locus_tag="PLU11_00855"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPN57681.1"
                     /translation="MNLILLIGAPLLTALAVLAARNKAQVKWISLAGSLFQLGLAVAL
                     LLAFRHERAINNTAQMLFEQQHLLFKTLNISFHFGVDGISVAMILLTSFVVVAGVLVS
                     WNVEKMTKEFYFLLLLLGLGAYGFFISLDLFILFFFLEISVIPKYLLIGIWGSGKKEY
                     SANKLALMLMGGSALILVGMLGLYFLAGNTFDIMTLSKTTIPAEYQHILFPLLFLGFG
                     VFTALFPLHTWVPDGHSSAPTAGSMFLAGISMKLGGYGCLRVATYLLPEGAKEYATII
                     IVLSAIAILYGAFATMMQKDLKYINAYSSVSHVGFSLLGIGMLTQTAITGALMQMVSH
                     GVMTALFFAVIGMIYDRTHTRMVGEMGGLMKQMPFISTIFFIVGLCSLGLPGLSGFVA
                     EMTVFVGSWQNPDLFHRIATIAAAMSIVVTAVYILRAIGKTMMGPNTNEAHLILKDAA
                     WNEKLAAILLVGGILAIGIAPGWLNDLLQPAAEEIMNRIAGK"
     gene            complement(8095..10089)
                     /gene="nuoL"
                     /locus_tag="PLU11_00860"
     CDS             complement(8095..10089)
                     /gene="nuoL"
                     /locus_tag="PLU11_00860"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPN57682.1"
                     /translation="MPNATNIALIPLLPLAAFVLLGLFGRKYLKSSAGLIGTALLLGS
                     ALLAFYTAYDYFFVYGKVDGVYQQFIPMKFTWLAFTKEMSIDMGLVIDPISVMMLVVV
                     TLISLMVHIYSLGYLKGEERFSTYYAYLGLFTFSMLGLVLASNIFQIYIFWELVGLSS
                     FLLIGFYYDKPSAVAASKKAFIVTRFADLGFLIGILVLSYCSGTFDFQTMISRLTDPE
                     SNEVKNAVASTFLGASALTWGAALVFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHA
                     ATMVVAGVYLVARLFPVFSVTTPGVLEVIGWVGAISAVIAAVIACTQTDIKRVLAYST
                     MSQIGYMMFALGVSKYGGEAGLGYTASMFHLFTHAMFKALLFLGAGAVIHYVHSNEMK
                     DMGGLRKKMPITHITFLVACLAIAGVPPLAGFFSKEEILLAAYHSNTTIYWIALITSG
                     LTAFYMFRLYFNIFWNKPYEAHGEHHSHGSASATSTQHASAGKEGGFVMMMPLVLLSV
                     GAAAAGFIPFGELVSTDGKALESHFSLSFSAAPVALGLAGILLALWMYAKNSDKPAKL
                     AASLGGLYKAAYHKFYIDELYLFITKKILFNIIGRAAAWFDRTIVDGLVNLTGNTTVL
                     VSEGIKKIQSGKVQQYAIIFLGGVILLAVLFIYVFKPVYF"
     gene            complement(10096..10410)
                     /gene="nuoK"
                     /locus_tag="PLU11_00865"
     CDS             complement(10096..10410)
                     /gene="nuoK"
                     /locus_tag="PLU11_00865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPN57683.1"
                     /translation="MMEPGLYHILFISTALFFIGVFGFLTRRNLITMLMSIELILNSV
                     NLNFIAFNKYVWPEKMDGLFFTIFIIAIAAAEAAVAIAIIINLYRNNQSIDVNNAEEL
                     KY"
     gene            complement(10407..10925)
                     /locus_tag="PLU11_00870"
     CDS             complement(10407..10925)
                     /locus_tag="PLU11_00870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPN57684.1"
                     /translation="MMNGYTILFYLLAALTLSCGIMSIATRHIFRAAIYLLFTLIGIA
                     GLYFWLSYEFIAAVQIVVYVGGIVVLIIFSIFLTQQAGEKLTRQKLDRKIWAALAIFC
                     GFALTMVQVLHYQFPLSNNPATVTVRRIGEKMMSMEAGGYALPFEIISMLLLAAMVGC
                     IVIATRSKPEVK"
     gene            complement(10922..11398)
                     /locus_tag="PLU11_00875"
     CDS             complement(10922..11398)
                     /locus_tag="PLU11_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN57685.1"
                     /translation="MFLIDYIKDVTGAVKSLATGMKRTLYYFTHHKEIITEQYPDNRA
                     TLVMAERFKGEVVMPHDEKNEHRCTGCTACELACPNGTIKVVTKFDITPEGKKKKAID
                     KLVYHLELCTMCNLCIEACPSDAIKMAQTFEHSVFNRSDLTKILNNPGSKIMEGVE"
     gene            complement(11580..12656)
                     /gene="nuoH"
                     /locus_tag="PLU11_00880"
     CDS             complement(11580..12656)
                     /gene="nuoH"
                     /locus_tag="PLU11_00880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPN57686.1"
                     /translation="MWSLAHIANEIHEWLSNTFNPTWTLVIEMVMAGVAVIGLFAGLG
                     LVLVLMERRVAAWMQIRLGPNRVGPFGLLQSMADTLKLIVKEGLTPDGADKVMFNIAP
                     FIAMIVAMLLMAPIAFAKDFQLWDLNIGVLYVTAVSSIMVISILMAGWSSNNKYSLMG
                     AMRSGAQIVSYELSAGLSIMTIIVLTGSLQISDIINAQSNGWWIFKGHLPVWISFVIF
                     IIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYINVFIVCAIGATLFL
                     GGWMPLHFGSNNAFNDAMDYIPSSIWFFGKTFFLIFLIMWFRWTFPRLRIDQLLNLEW
                     KYLLPISMINLLLATAMAILGWHF"
     gene            complement(12792..13934)
                     /locus_tag="PLU11_00885"
     CDS             complement(12792..13934)
                     /locus_tag="PLU11_00885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPN57687.1"
                     /translation="MYRETQIIKETSPGIVGETGEFTINVGPQHPATHGVLHLVITLS
                     GETIMKVEPHLGYIHRSIEKMCESLTYRQFIYVTSRMDYLSSHINNHACALCVEKGMQ
                     IEVPERAKVIRVLMDELTRIASHELWWGAMAMDLGAFTPFFHAFRERESINEIMEETC
                     GARLTMNYNVPGGVMQDLHPDFQRKVKEFLKLYKSKIHEYDELVTGNIIFQNRMKGVG
                     ILSAKDAISYGCSGPTARGSAVSSDIRKVYPYGIYDKLEFDEVLETGCDSFARYMVRI
                     REMQQSIRIIEQLIDNIPEGDFQAKTKAVLKLPKGEFYTKVETARGELGVYIVSEGGT
                     TPYRIKFRSPGFSNLSVLDHIARGSKIGDLVAMMGTLDLVIPDIDR"
     gene            complement(13955..14413)
                     /locus_tag="PLU11_00890"
     CDS             complement(13955..14413)
                     /locus_tag="PLU11_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN57688.1"
                     /translation="MSKELLKFRITEMLPSASFDESGEWLNVEVTAAEWPTLAGALCK
                     DSHLALDYMFCLTCIDWKTHLTMVYHLHSTSFRHNIVVKVKLDRAKPEVVSVCKCWRT
                     AEFHEREVYEMFGVNFLNHPDLRLLILPDGWEGKNPLRKDFEDPINMIRL"
     gene            complement(14416..14952)
                     /gene="nuoB"
                     /locus_tag="PLU11_00895"
     CDS             complement(14416..14952)
                     /gene="nuoB"
                     /locus_tag="PLU11_00895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPN57689.1"
                     /translation="MSVKQNTANDFPGQIHENPGGGIVLSKLDDIINWARSNSLWPLT
                     FATSCCGIEYMAVAGAKYDFSRFGFEVARATPRQADVIIIAGTIVNKMAPVLKRLYDQ
                     MADPKYVIAMGACAISGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALLHALISL
                     QNKIKAGMTREQIRTDKD"
     gene            complement(14967..15317)
                     /locus_tag="PLU11_00900"
     CDS             complement(14967..15317)
                     /locus_tag="PLU11_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPN57690.1"
                     /translation="MGASSLIVLIIAAVAFSGIAIMLSTFVLKATKNKQKGEPYECGI
                     PTKGKTWIQLNVGYYLFALIFLIFDVELVFLYPWAVVAKSMGWVALVEIVIFFFILFT
                     GFLYAHKKGALKWV"
     gene            complement(15428..16147)
                     /locus_tag="PLU11_00905"
     CDS             complement(15428..16147)
                     /locus_tag="PLU11_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN57691.1"
                     /translation="MALLPWRKGIVTKIDDHTHNTRRFWIQVPELERFDFMPGQFVTL
                     DLPIHEKPNKRWRSYSISSWPDGSNIFELLIVHAEGGSGTSYLFEEINVGSELVFRGA
                     QGVFSLPPQLDKELFLICTGTGIAPFRSMAQYIHLQKVPHQKVHLIFGCRNKADLLYF
                     DELMELEEKMPGFRFHPVLSREHWEGHNGHVHEVYQELCKERQDAYFFLCGWKQMIDE
                     AKVVILEMGYDKKTIHQELYG"
     gene            16250..19684
                     /gene="mfd"
                     /locus_tag="PLU11_00910"
     CDS             16250..19684
                     /gene="mfd"
                     /locus_tag="PLU11_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPN57692.1"
                     /translation="MSVQELLQKYHQTPRLFQLADRLSFARPEKIYLENLRGSASQFI
                     VAAAFTHPSLSQMNHLVILNDGEEAAYFHNTLENLTEALDIFYFPASFKNRKNYQLLN
                     SSHVMLRTEALTKIAAGGNKKVLVTYPEALFEKVVLPKTLSSNIIHIRTGDTLDTGQL
                     LLKLGDYGFERTDFVYEPGQFAIRGGILDIYSFGNEKPYRVELYGNEVDSIRIVDPET
                     QLSERKLLQVSIIPNVDTQFEEEDRISLFEFLPENTVIWIQDYALCREHIADSIEDLE
                     SFLRIQEEIRAQPVVKTKHREELDDKLEKKQVSMKDFISIPEWEEKIVQRHLVGFGHG
                     DSFPDHFSPLTIRHNTREQPSFNRQFDLLIRDLKSWEAKKFQLYIFAENPRQLERMQS
                     IFDDLKADIVFNPVSTSIHEGFIDEDLQLLCYTDHQVFQRYHKYKVKQAYNKNKALTL
                     RTLRELQPGDFVTHIDHGVGVFSGLQKIEVNGRTQEAVRLIYKDTDILYVNINSLHKI
                     AKYTGKDGSVPKVNKLGSDVWNKLKEKTKAKVKEIAFDLIKLYAKRKAQQGFSHTPDN
                     YMQTELEASFIYEDTPDQSKAAADVKKDMESPSPMDRLVCGDVGFGKTEVAIRAAFKT
                     CLDGKQAAVLVPTTILAFQHYKTFKDRLKDFPVTVDYINRFKSAKERKETLKKLEEGK
                     IDIIIGTHGLLGKEVKFKDLGLLVVDEEQKFGVGHKEKIKTLRTTVDCLTLTATPIPR
                     TLQFSLMGARDLSIMNTPPPNRQPIQTEVQVYNEDFIRDAIYFETERGGQVFFIYNRI
                     AGLAEMAAIIQALCPDLSIGFAHGQMEGHELEEKILDFIDRRYDVLVCTNIVESGVDI
                     PNVNTIIVNNAHQFGLSDLHQLRGRVGRSNKKAFCYLLAPPMSTLPNDSRKRLQTLEQ
                     HSELGSGFQIAMRDLDIRGAGNLLGGEQSGFMAEIGFEMYQKILDEAIRELKRTDFKD
                     LFKEEIARQDDFVQDCTIDTDLEILIPDEYVESIAERLSLYQRLDNAESEEELLAMKT
                     ELEDRFGTLPRQVDDLFITVRCRKLAVDLGFEKMSLKDNTLRCFFINRPDSPYFESGT
                     FQKIIQFLQTETNKGKLKQTGRLFLLVANDLQDMAAMHRFLAAMHRYCFPAPVATV"
     gene            19770..25187
                     /locus_tag="PLU11_00915"
     CDS             19770..25187
                     /locus_tag="PLU11_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN57693.1"
                     /translation="MRIYPIPLIAFLLVTCFTATAQQDARFALLLKSGAVFPTKNIST
                     SRVDSFNNRSSRTAGKSFAILQFEQLPTAAERQQLLQAGIELLDYIPNNAYTATITGL
                     LSEATLQRVQARAIIEPSPQQKMQPQLARGLFPTYAIKTPGTIDLWISYPKSFLPEQV
                     KEELRRGNYDLVSNDYQLYRILGVRIAASRLEELAATPWVEYVQAIPAPDRELNSNSM
                     YTTRASVLRAPLSAGGRNLDGQGVVVGVGDNGDIQSHLDFNGRLINRAADIMRAHATH
                     VAGTIGGAGIIQELYTGYAPKATLLTQYYSGIFTEAPAYVQDHGMVITNNSFGAVVDD
                     CFFNGLYDLTSRVLDQQAIDLPELQQVFAAGNNGALTCSPYPAGFKTVLGGYQSAKNV
                     LTVGSTDFRSNIAASSSRGPVKDGRVKPEIMSQGVFLASTWTSNIYSYNNGTSMAAPG
                     VSGGLALLVQRYRQLNAGANPKNGLSKAILVNGADDKGNSGPDFRYGFGRLNLVRSLA
                     MIEGNTYFNSTVANTVTNTHTISVPANTAKLKVLVYWQDPPAAVMASKTLVNDIDLEV
                     NTPASFTVFPSVLDTLPANVNNLAASGTDHINNIEQVEINNPTAGNYDLKVIGNAITQ
                     NPSQEYFLVYDIIPRSLVLTNPVGGDKFTPSVTTLDTVYIQWDDYGSSGTYTLEFSPD
                     NGSSWILLNNAVSASSRVYSWGVPNTPTDQGRIRITNNTSGLTQTSAAFTIATLNTVS
                     LDAVQCEGYIKINWTAVPGATDYEVMWLQGTEMVSVTTTAALTYTFSGLSKDSTYWVT
                     VRPRINGNPGRRANAISRQPNAGTCAGTISDNDLKLDAIVSPATSGRELTSTALSATH
                     AVTIRIKNLDDAISSGNINVSYSINGGPAVNEVITTPIADIAAGGTLSHTFATTANLG
                     ATGTYSIEVIATKASDPVTANNTQTKVFKQLANAAINNALLPWLDDMETAAEQTITVD
                     QMGLTGNDRYDFVNNGIYGQAKTFIHTGIAYSGSKALTLDQSRFVSGGSVDSLTGTFN
                     LATFNTATDDIRIDFRYKNHGQKSNAANKVWIRGNDTDNWIAVYDLYANQPAADGSFK
                     LSGSIELSDSLAAYGQSFSTSTQVRWGQWGEYLAADNFRAAGYTFDDIRLYKAVDDIQ
                     LVSVDTPYTVSCGLSAAVPVKVTVRNTSNAIINNIPITLKVDGVVIANETITSIAASS
                     SVQYTFTATANLTTSGNHIIEVWSALATDTYNQNDTVSSTVNNLPLVSSFPYLQNFET
                     NNGSWYTGGILSSWEYGTPISPKINRAASGSKAWKTRIAGYYNDNEFSYLYSPCFDIS
                     TMTNPTLSFSVAMDIEDCGAVLCDAAWVEYSEDGITWTKLGSFGQGTNWYNKNYAGNQ
                     LWSQQNYTNWHVATTALPTSNNSRLRFRFVFNSDSGLNKDGIAVDDIHIYDNLYGLYD
                     GATMAAPVTQTINTGTNTWVDFLEGGKLVASVHPAGQEMGATDVRAYLHTGAVRWSSN
                     QYYHNRNFTIQPANAFLALGDSVLVRVYFPDTETETLIAATGCSGCAKPATVYDLGVT
                     KYSDPDNSYENGTIADNNQGVWSFVIPSRATKVPFDKGYYAEFKVNNFSEFWLNTGGL
                     TGSQPLPVELKQFTVRKLPQDDVLAEWVTASEYNVNRFEIEVARGNADYQSGRYTRLG
                     EILSQGNSDREQRYQYLDTEPGKSGVRYYRLKIIDNDGTVRYSAIRPLVFNIEIQWTV
                     TPNPSAGQFSLNCQASPGETIQVKVYDTKGRLVYQHSNPASGFAQKLNIDLKGPAYPA
                     GIYLLETGVGEKRRSFKLVKQ"
     gene            complement(25372..25575)
                     /locus_tag="PLU11_00920"
     CDS             complement(25372..25575)
                     /locus_tag="PLU11_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57694.1"
                     /translation="MSDVKITAYNVKTKEKGVEMLDAVITKTAKGAYMAQGNDGKGNK
                     LTTLMGEEKALAAIKAGIAKQGW"
     gene            complement(25755..>26342)
                     /locus_tag="PLU11_00925"
     CDS             complement(25755..>26342)
                     /locus_tag="PLU11_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPN57695.1"
                     /translation="LRGKILNVEKAMEHKIYDNEEIRNMFTALGVKMGTPEDPKALDL
                     SRLRYHKLIIMTDADVDGSHIATLILTFVFRYMTELVEQGYVYIAQPPLYLVKKGKEQ
                     AYAYNEDQRKLWVEKLGGGKDDSVNIQRYKGLGEMNASQLWETTMNPETRTLKRVTIE
                     SAAEADRVFSMLMGDEVAPRREFIESHAKYAKLDI"
     assembly_gap    26343..26442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26443..27362)
                     /locus_tag="PLU11_00930"
     CDS             complement(<26443..27362)
                     /locus_tag="PLU11_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN57696.1"
                     /translation="MTTEAQEKAAQISAGYSADSIQVLEGLEAVRKRPAMYIGDIGVK
                     GLHHLVYEVVDNSIDEALAGYCKNINVAIHEDNSIAVEDDGRGIPTAMHSKEKKSALE
                     VVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSKLLVTVNRDGKIFEQEYRIGVP
                     QYAVRETGTSETTGTRVHFWPDDSIFITTTYNKDILESRLRELAYLNRGVRIILTDLR
                     EKNEDGSNYSKEFYSEGGIVEFVEMLDANAGRNTLIPKVISVEGRDEANNVAVEVAMN
                     YNDSYNEHIYSYVNNINTIEGGTHVSGFRRA"
     gene            complement(27808..>28473)
                     /gene="fabZ"
                     /locus_tag="PLU11_00935"
     CDS             complement(27808..>28473)
                     /gene="fabZ"
                     /locus_tag="PLU11_00935"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPN57697.1"
                     /translation="KSEGYLNNLELRFPNEPARHKLLDVIGDLALIGYPIKARIIANR
                     PGHSSNVDFARKIKQYIKKNKHTQGIPVYDPSQPPVYDLQYIEKTLPHRFPFAFVDKV
                     IELSDTHIVGVKNVTFNEWFFQGHFPGNPIMPGVLQIEALAQCGGILAINLSGDGQYD
                     TYFLKIDNCKFKQMVRPGDTLILKMELAAPIRRGICEMKGTTYVGNKVCCEADLVAQI
                     VKR"
BASE COUNT         7577 a         6799 c         6600 g         7397 t
ORIGIN      
        1 tagtttggtg ttatcaccaa caagtatcac ttcaccgcca atcccttcag ccttaacccg
       61 attgtagccc ggctgatagt ctgctggttg ccagatgatc ctcctgtata agagactatc
      121 tcaacatcat ataatgaaaa actatacaat actcccttca ggtctggtgc caatagatgg
      181 atgttgccct tttcttttct gagcccagct gctcctgtaa gaaaccattg attccaggca
      241 gcgccatcaa tagccattac ttcaatggtt aaattaatgt aggatccgtc acccggctta
      301 atacccaatc cacttatttt agccacccgg gttgttggca gatttcctag tgttatactg
      361 aagttggcag ttaaggcccg ccgtttattc ctgggggcca ggtcaggcct ggtaccgcct
      421 ttttgtattt taactgaacc cgctctgatt tttaccgtcg ctttaaaagg ggccttggat
      481 gtagcatcca gtgcattaaa gaggatttct tctaccaccg cattgtcgta aaatctttcc
      541 tgtaaaggat caccagcata gttaagcgtg acaaaattca actgccggct cgcctgttct
      601 tttgtataga cctggagtat cctgagaata ttctgagtgg ccgcatcatc ggatagaaaa
      661 aagtcaaagc tggctgtgtg ctggagttga ttttcaggcc ccagcgcatc agtgaagctg
      721 agattttcca cctgaatatt gtttttttcc agttgcaact gggtggttga ttgtgcataa
      781 gtggttgtct tttgcgcaaa cactgatacg ttaaatactg aaagcaataa aagggaaaga
      841 cagaattgaa ttgacttttt catcgccggt tttttagtag gaagtaaaac taagagatga
      901 agattacccg gagtatccct gtttcagggg atatttgata atatgaagag caggtaaagc
      961 tgaatgtacc cggaagattt gtaattaaat gtcatatttt tcgagaaatc cccaattgtc
     1021 tttccctgtt tcataaaaaa gggcagaaga atagtggtag tcttccggat ccttgcaaaa
     1081 cccttttttt acaggattat catgtatata ctgaagtttt tgcaggtaaa caggagctgt
     1141 ccataatgcc tgactgtatg gatttctttc ccataactga tatttacggt ctttactgtc
     1201 taccctgaat aactcaagaa atccgggatt ggttttttgc atagaaaatt taatctgttg
     1261 cccggtaaat ttcaggaaat cacgctgtac attttccggc cggtgatccg gttttatttt
     1321 ccagatcagg tggatatgat tggacatcag gacaaaggcg tgcatgctga ttcgtctttt
     1381 ctccaccaaa taccggaggc tgctgattac tatttcttta tgcgcatcat cttttagaag
     1441 tggtttccac caaagatttg ttgcagtgaa gaactgaggg cattcgggtt ttaatactga
     1501 catgccgtaa ggtaaattcc ggaattggag gctgtatggg aaaaagacgt agacgggaag
     1561 tgaaaattcc ctcatacgga tgttcaaaaa aagccaactg attggtgttt ttagtaataa
     1621 atgcaccgga gacaggatgt atatcgttgg tgttctcacc aacgatccag cgcttatagg
     1681 tgtgattgct ggttatatca ccaaccgttt tgttggtgat aacaccaaca aaacgaaacc
     1741 ctctcaaaga cttgagaggg tttctatcgg attatagtga gtgagaaaac tgcatcttgt
     1801 tggtgttatc accagctgct gcatcttgtt ggtgttatca ccagctgctg catcttgttg
     1861 gtgttatcac caacaagctt acttcttctg ctttctcggt gtttcaatcg cagcctgtgc
     1921 cgcagccagg cgggcaattg gtacacggaa cggagaacag cttacatagt tcataccgat
     1981 acggtgacag aatttcacgc tttcgggatc gccgccatgc tcgccgcaga tacctacttt
     2041 gaggttgggt ttggtcaccc ggcctctttc tgtacctact ttgatcagct ggccgatacc
     2101 ttcctggtcg atggtctgga acggatcatc gggaaggatc ttcaggtcaa ggtattttgg
     2161 taagaaacca ccaatatcat cgcggctgaa accaaagctc atctgggtga ggtcattggt
     2221 accaaagctg aagaagtcgg ctacattggc catgaattca gcccgtaatg cagcacgcgg
     2281 cgtttcgatc atcgtaccat agaggtaggg gatcttcttc atcttgaatt tctcacatac
     2341 ttccttgtat acctgatcca cgatcacttt ctggtgacgt agctcatgag caatcactgt
     2401 aacaggaacc atgatctcag ggaaagcttt cttacctgct ttcagtaatt cggcagttgc
     2461 ttcgaaaata gcgcgtacct gcatctctgt gatctccgga taactgatac cgagacgtac
     2521 accacggtga cccagcatcg ggttattttc atgcaaagcg agtacacgtc tgttcagcat
     2581 acccatactg ataccgagtt ctttgctcag ctggcggagt ttttctttat catggggaac
     2641 aaactcatgt aatggtgggt cgagcagacg gatggttacg ggatgacctt tcattacgga
     2701 aagggtattc ttcacgtctt tcttcacaaa cttgaacagg ccgttcagcg cttctcttct
     2761 ttctttttcc gttttgctaa cgatcatttt acgtagctgg aacaggggtt cttcgcttcc
     2821 ttcgccatag aacatgtgtt ctgtacggaa gagaccgata ccttctgcac cgaatttaac
     2881 ggcgtttaca gcatccagcg gggtctctgc attggcacgt acgccgatct gtttgaattt
     2941 atccaccagt ttcatcaggt ctttgtagga ctggttcttg tcgatatcaa tgtccaccag
     3001 ggccatactg ccttcataaa cggcaccttt agtaccattc agactgatcc agtcaccttc
     3061 tttgatcacc acaccattct ttgcatggaa gtttttggct tccgcatgga tttcaatatc
     3121 gccacaacct actatacagc atttacccca gccgcgggct acgagcgctg cgtgagacgt
     3181 cataccacct ttagtggtaa ggatcgcttg tgctttatgc ataccatcca catcttcggg
     3241 ggaagtttct tcacgaacga ggattacttt ctcccctttg gaggcccatt ctactgcttc
     3301 agcagaagag aagaccacgc ggcctttggc accaccagga ccggcaggaa gacctttggc
     3361 aataacagga gccagtaatt ctgctttagg atccagcatt ggcaggagta attcaaccag
     3421 ctgattcggg cccacacgca tcaatgctgt tttggcatca atcagtcttt ctttgaccat
     3481 ttcagtggcc atacgaaccg cagcgacacc gttccgttta ccaacgcggc attgcagcat
     3541 atagagtttt cctttttcaa tggtaaactc aatgtcctgc atatcatgat agtgcttttc
     3601 cagtctgcgc tgaatggtat atagctcttt gtattgagca ggcatcagtt cttcaagggt
     3661 ggagaaatgt tcactctgtg cattctttga gtagtcattg acaggagaag gagtccggat
     3721 accggccacc acatcctcac cctgcgcatt tatcaggtat tctccataaa aatggttttc
     3781 gccattaccg gggttacggg taaatgccac accggtgcag catccttcgc ccatattacc
     3841 gaataccatg gcctgtacgt taaccgccgt tccccattcg tgtggtattt tttcaatacg
     3901 acggtattca atggcacgtt taccattcca gctggcaaat acggcaccca caccacccca
     3961 catttgttcc cagggatcat cagggaattc agtgcccagt actttcttaa cggttttctt
     4021 ataatcttta acgagttctt tcagttcttc agcagtaagg tccgtatcgc tggcatatcc
     4081 tctcgtcttt ttaacatgct ccagtttttc atccagcact ttacgaatgc ctttacctcc
     4141 tttgggttcg ataccggcgg ctttttccat caccacatcg gcatacatca tgatcaggcg
     4201 acggtaagcg tcataggcaa aacgggcatc accactctgg gcaatcacgc cttttatggt
     4261 attttcgttg agacctacat tcaatacggt ttccatcatg ccgggcatac tcttacgggc
     4321 accggagcga acggataaca ataatggatt tttttcatca ccgaattttt taccggtgat
     4381 cttttccatt ctttccatgg attcttttac ctgtgcgttc agggatttgg gataggttct
     4441 tttattcgca tagtagtaag tacaaacctc tgtggttacg gtaaatcccg ggggaaccgg
     4501 taatttaagg ttcgggtgtc ctgccatttc ggcgaggttt gcccctttgc cgcccagcaa
     4561 gtttttcatt gattcgtttc catcggcctt gccgccgccg aagaagtaca catactttcc
     4621 ggtttttgtt tttgttgcca tattagataa gcttttgtgc ttgcatagtt agcgctaaac
     4681 cagccgaata gggggggaca caggtcagcg gaaacactga ttcttatcaa tgacaaacat
     4741 gaaaaaagac gaaatggtat ttatttcgtc tttttcaatt agttatccgg taaaaattta
     4801 tcccttgaac tcgtagtaag gcacttcctc atggctctcc gcttcttttt gcagccaata
     4861 agcatgggtc accacatgtc cattcttggt tttcagcatc cggccgaaaa tggcattggt
     4921 ggcattgaaa gtataatccg ttacgccaac tgtaaatgtt gaggggggcg ttggtggtgg
     4981 cggaggaggg ggcgctgttt ctccctctgc cactttggga ggcggggccg cttttggcgc
     5041 cgggctgggg acagacacta ccgtaaaccc gttcaattcc agtaatggtt ttagaaaagc
     5101 gagtctctcc ggacttacat tcttttccac gatgccgcat ttcacgccat ccagttcctc
     5161 aaattcatgg ttcttgttga tcgccataca ctgtgcttat aatgaatgaa tgtaatcgta
     5221 gatccagctc agtaatccgg ctagtacaat accggcaccg caaagaagca tacccagttt
     5281 taccggggcc atcagtttca gcggttcaat cggattatcg ttcttatcca taaataccgc
     5341 ccggatcacc cgcaggtaat aatacaggga aatgatcata ttgagggccg ccaccaggat
     5401 gaaccagtaa gccgcaccgg aagcgccggc cgtgatcagg aataattttc caaagaagcc
     5461 agccgtgggt ggtataccag ccagggagaa taaggccaga gccagtatcc agctcataaa
     5521 aggattggtt ttatacagcc ctttataatc atcaatattt tctttgccag ttctttcggt
     5581 tatcacactg gccaccccga atgcggcaag attggagaac aaataaatca gcacaaaata
     5641 aatgacggca gtgcttccca tatggaaatc atcgctgcta agggccagta atataaaacc
     5701 aacctgggcg atggaagaaa atgccaggaa acgtttgata ttctgctgac ggagcgcaaa
     5761 aagattaccc accaccatcg tcaggacaga aagaatgatg atcatgacct gccagacctc
     5821 tttcatcgga gtaaataccc ggaagagcat ggtcatgaaa ataaaggcaa cggccccttt
     5881 cgaaataacg gagaggaatg aagtaaccgc aataggtgaa ccttcataca catcggcggt
     5941 ccacaaatgg aagggaacca tggataattt gaaggcaaat gccgtaaaga agaatataaa
     6001 tgccagaatc tgcagggttg aaccgtccag gcgttcctgt atttcaggaa gactgataga
     6061 accggtactg ccatagatca gggagattcc aaagagtaag atgccggagg agaaagcaga
     6121 agagagaatc atcttcatgg caccttccgc ggattttttc ttgtccagat caaaatttgc
     6181 catcgccgca acaggaatgg ttgcgagttc aagcgccagg tagaaaatca gcaggttggc
     6241 actggagatc ataaagaaca tacccaacag ggccgacagc atcaagagga gaaattcagc
     6301 caggtgtggt gtttttctca gccagtccgc acatagcagg gtgatcaggt ataccccaag
     6361 gttcagaatg tttttctgga aggcaataag atgagtggta tagaacatgt ccccaaacaa
     6421 gcggccctct gtattcagga caaagccaaa aacaaaatta gccagtagta aaacctgtgc
     6481 caatggcagc agggtttcat tcttcacccc tttaccgatt ttgataaaga gtaaaaggaa
     6541 aatgatgccg gtaagcacca gttctgattt catcaatgtg aacagttcat tcattccagc
     6601 cagttttatt ttccagcaat tcggttcata atttcttctg ccgccggttg cagcaggtca
     6661 ttcagccagc ctggggctat cccaatggcg agaataccac ccaccagtaa tatggcggcc
     6721 agcttttcat tccatgcagc atcctttaag atcaggtgtg cttcgttggt attgggcccc
     6781 atcattgttt tgccaattgc tcttaatata tacactgcgg tcaccacaat ggacatagcg
     6841 gcagctatcg tagcgatccg gtgaaagaga tccgggttct gccaggaacc tacaaatacc
     6901 gtcatctctg ccacaaagcc gctcaacccg ggtaaaccga gtgaacaaag tccgacaata
     6961 aagaagatgg tactgataaa gggcatctgt ttcatcaggc ctcccatctc acctaccatc
     7021 cgggtatgcg tacggtcgta gatcatgcca atcacggcaa agaaaagggc cgtcattacc
     7081 ccatgtgata ccatctgcat cagggccccg gtgatagccg tttgcgtcag cataccgatt
     7141 cctaacagac tgaatccaac gtgactcacg gaagagtagg cgttgatata tttcaggtcc
     7201 ttttgcatca tcgtggcaaa tgcaccatat aagatagcaa tggcagatag tacaataatg
     7261 attgttgcat attctttggc gccttcgggt aataagtagg tggctacccg taaacatcca
     7321 taaccgccga gtttcatgga gataccggcg aggaacatac tacccgcggt tggagcggaa
     7381 gagtgtccgt ctggtaccca ggtatgcaac gggaataaag cggtaaatac cccaaagccc
     7441 aggaataaaa gggggaataa aatatgctgg tattcggcgg ggatggttgt tttggaaagg
     7501 gtcatgatgt caaatgtatt acccgcgagg aaatataatc ccagcatacc caccagtatc
     7561 agggccgaac cacccatcag catcagggcc agtttgttgg cactgtattc ttttttacca
     7621 cttccccaga ttccgatcag caggtactta ggtattacag atatttccag aaagaagaag
     7681 agaatgaaca gatcaaggga aatgaagaaa ccataagcgc cgagtccgag cagcagcagc
     7741 aggaaataaa attccttggt catcttctcc acattccagg aaaccaacac tccggcaacc
     7801 actacaaagc ttgtcagcag aatcatggcg actgaaatac catccacgcc aaagtgaaaa
     7861 ctgatattca gcgttttgaa aagcaggtgc tgctgctcaa acagcatctg ggccgtgtta
     7921 tttatggctc tttcatgccg gaatgccagc agtaaagcta cggccaatcc gagttgaaat
     7981 aaagaaccgg ccagtgaaat ccatttcacc tgtgctttgt tccgcgcggc caatacagcc
     8041 agggcggtta acaaaggggc tcctataagt aatataaggt tcatcaattc ttatttagaa
     8101 atacaccggt ttgaaaacat aaataaacaa tacagccagc aggatcactc cacccaggaa
     8161 gataatcgca tattgctgca ctttcccgga ctggattttt ttgattcctt cggataccaa
     8221 tacggttgta ttacccgtga ggttgaccag tccgtccaca atggtgcggt caaaccaggc
     8281 agccgcacgg ccaatgatat tgaacaatat ctttttcgtg ataaacaggt ataattcgtc
     8341 aatgtaaaat ttgtgatacg ctgctttgta taaacctccc agtgaagccg ccagtttagc
     8401 tggtttatcg ctgtttttgg catacatcca aagcgccagt aaaataccag ccaatcccag
     8461 ggccaccggg gctgcagaaa acgacaggct gaaatgggat tcaagtgctt tgccatccgt
     8521 gctgaccagt tcgccaaaag gaataaaacc agcggcggcg gcacctacag ataatagtac
     8581 aaggggcatc atcataacaa agccaccttc tttgcccgcc gaagcatgct gtgttgaagt
     8641 tgcggaggcg gagccatgac tatgatgttc accatgcgct tcatatggtt tgttccagaa
     8701 tatattgaag tataaacgga acatataaaa agcggtcagt cctgaagtaa ttaatgcgat
     8761 ccagtatatt gtcgtattac tgtgataagc cgctaacaga atttcttctt tgctgaagaa
     8821 tccggccaat gggggaacac ccgcaatagc cagacaggcc accaggaaag tgatatgcgt
     8881 aatgggcatt ttttttcgta atcctcccat gtctttcatt tcattactgt gtacataatg
     8941 tatgactgca cccgcaccga ggaagagcag ggctttgaac atggcatggg taaacaggtg
     9001 aaacatagag gcggtatagc ccaatccggc ttcaccaccg tattttgaaa cgcccagtgc
     9061 aaacatcatg taacctatct gcgacatggt ggaataagcc agcactcttt tgatatccgt
     9121 ctgtgtacag gcgattacgg cagcaatcac tgcggaaatt gcaccaaccc aacctatcac
     9181 ttccagcaca ccgggggtgg tgacggagaa gacagggaat aatcgggcta ccaggtatac
     9241 acccgctacc accatggtgg cagcatggat taatgcagaa acaggcgttg gaccttccat
     9301 ggcatccggt aaccagatat gcaagggaaa catcgcactc ttaccagctc cgcctataaa
     9361 tacaagggct gcaccccagg tcagggctga agcacccagg aaagtggaag ccactgcatt
     9421 tttgacttca tttgattccg gatcagtcag ccggctgatc atcgtctgaa aatcaaaagt
     9481 gccggagcaa taggagagta ccaggatacc gatcaggaaa ccgagatcgg caaaacgggt
     9541 tacaataaat gcttttttac tggccgccac cgcgctgggt ttatcataat agaaaccaat
     9601 cagcaggaag gaggataatc caaccagttc ccagaaaata tagatctgga aaatattact
     9661 ggccagtacc agtccgagca tggagaatgt aaataaaccc aggtaggcat aataagtgga
     9721 gaatcgttct tcacccttca ggtaacccag actgtaaata tgtaccatca gtgaaatcag
     9781 ggtcaccacc accagcatca ttacggaaat gggatcgatc accaggccca tgtcaatcga
     9841 catttccttg gtgaaagcca gccaggtaaa tttcatgggt ataaactgct ggtaaacgcc
     9901 gtcgactttc ccatacacaa agaaataatc ataagcggta tagaaggcaa ggagagccga
     9961 tccaagcagg agggcggtgc caatcagtcc cgcgctgctt ttcaggtatt tccggccgaa
    10021 taagccaagc agaacaaagg cggccagggg aagcagggga atcagtgcta tgttggttgc
    10081 gttcggcata gtctgttaat actttaattc ttcagcatta tttacatcaa tggactggtt
    10141 gttccggtac aaattgatga taatggcgat ggctacggcc gcttcagctg cggcaatagc
    10201 gatgatgaat atggtaaaga aaaggccatc cattttttca ggccatacat acttgttaaa
    10261 ggcgataaag ttcaggttca cgctgttcag gatcagctcg atactcatca gcatggtgat
    10321 caggtttctg cgggtcagga aaccaaatac cccgataaag aataaagcgg tgctgataaa
    10381 taaaatatga tataatccgg gttccatcat tttacttcgg gtttactgcg tgtagcaatc
    10441 acgatacatc caaccatggc tgccagcagc agcatggaaa ttatttcaaa cggcagggca
    10501 taaccacctg cttccatact catcattttt tcaccgatcc ggcgtacggt aaccgtagca
    10561 gggttattgg acaaaggaaa ctgatagtgt aatacctgca ccatcgtcaa tgcgaatccg
    10621 caaaagatcg ccagggctgc ccatattttg cgatccagtt tttgcctggt taatttttcc
    10681 ccggcctgct gtgtcaggaa gatggagaaa ataatcaaca caacaattcc gcccacatag
    10741 accacaattt gtacggccgc tatgaattca taggagagcc agaaatagag acccgcaata
    10801 ccgatgaggg taaataataa atagatcgcg gcccggaaga tatgccgggt ggcgatggac
    10861 atgataccgc aactgagggt cagtgccgcc agcaggtaaa aaagtatggt gtatccgttc
    10921 atcattctac gccttccatg attttagaac ccgggttatt taaaattttt gtcaggtcac
    10981 tccggttaaa cacactgtgt tcaaaagtct gggccatttt gattgcatca gaaggacaag
    11041 cttctataca gaggttgcac atcgtgcaga gttccaggtg gtataccagt ttgtcaatgg
    11101 ctttcttttt ctttccttcc ggggtgatat caaatttggt gaccactttg atggtgccgt
    11161 tgggacaagc cagttcgcag gccgtacaac cggtgcaccg gtgttcattt ttctcatcat
    11221 gcggcatcac cacttcaccc ttgaagcgct cagccatcac cagcgtagcc cggttatccg
    11281 gatactgctc ggtaatgatc tccttatgat gggtaaaata atacagggtt cgtttcatcc
    11341 ctgtagccag ggatttcacc gcaccggtaa cgtcttttat gtagtcgatt aaaaacattt
    11401 tatgtttaat tcattttatt aaatgtcgcc cttccattac ctcaccccaa cccctctcct
    11461 ttaagagagg ggctagaaga tattctttct tccttgtcct cttgctcttt tagcatcagc
    11521 cgatttaaaa gacctttccc cttatcacgc ccccattcac cattcaccac tcacctccct
    11581 caaaaatgcc atcccaatat agccatcgcc gtagccagca gtaaattgat catactgatc
    11641 ggcagcaggt atttccattc caggttcagc agctggtcga tccgcaaacg cgggaaggtc
    11701 cacctgaacc acatgatcag gaagatcagg aagaaggtct tcccgaaaaa ccagatactg
    11761 gacggtatat aatccatggc atcgttaaat gcattgtttg atccgaaatg cagcggcatc
    11821 cagcctccca ggaataaagt ggctccgatg gcacatacaa taaatacgtt gatatattct
    11881 gccaggaaga aaagtgcaaa cttcatcccg gaatactccg tatggaaacc cgcagtcagt
    11941 tctgactcag cttcagccag gtcaaagggc gcacggttgg tttctgccgt tacggcaatg
    12001 ataaagatga caaaggaaat ccagacgggt aaatgacctt taaagatcca ccagccattg
    12061 ctttgagcat taatgatatc ggagatctgc agactgccgg tcagcacgat aatagtcatg
    12121 atggataatc cggcactcag ttcataactc acgatctgtg ccccgctgcg catggcaccc
    12181 atcagggaat atttattgtt actggaccag ccggccatta atatactaat gaccataatg
    12241 gaagaaacgg ccgtaacata taagacgccg atattcagat cccacaactg gaagtctttg
    12301 gcaaaagcaa tgggggccat cagtaacatc gccacaatca tggcaataaa gggagcgatg
    12361 ttgaacatca ctttatccgc gccatcaggt gttaatcctt ctttgacaat taatttcagg
    12421 gtatccgcca tggattgaag taaaccaaag ggaccaaccc ggttagggcc gaggcggatc
    12481 tgcatccagg ccgccacccg tctttccatc agaaccagta ccagtccgag accggcaaat
    12541 aatccaatga cggccacgcc ggccattacc atttcgatga ccagggtcca ggtgggattg
    12601 aatgtattgc tcaaccactc atggatttca ttggcgatat gtgcgagact ccacatgctg
    12661 cattgttcag gttagtatga ttgaccggtg caactgttta gcagtcgccg gtactttatt
    12721 ttcattttga ccggacagtg acaaacccct tgttcgcagt aaaacccctg tttactttag
    12781 ttaaacagtg attacctgtc gatatcgggg atgaccaggt ccagtgttcc catcatggcc
    12841 accaggtcac caatcttgct gccacgtgcg atatgatcga gtacggacag gttggaaaaa
    12901 ccaggtgaac ggaacttaat ccggtaagga gtggtgccgc cttcacttac aatataaaca
    12961 cccagttcgc cacgggcggt ttccactttg gtataaaatt cacccttggg cagtttcagc
    13021 acagccttgg tcttggcctg gaaatcacct tcgggaatat tatcgatcag ttgttcaata
    13081 atgcggatgg attgttgcat ttcacggata cgtaccatat aacgggcaaa ggaatcgcaa
    13141 ccggtttcga gcacttcgtc aaattccagt ttgtcataaa taccgtacgg gtatacctta
    13201 cggatatccg aagaaacggc acttcctctt gccgttggac cactgcatcc gtaagagata
    13261 gcatcttttg cactgagtat gccgactcct ttcatccgat tctggaaaat gatgttaccg
    13321 gttaccagtt catcgtattc gtgaatttta cttttataga gcttcaggaa ttccttcacc
    13381 ttccgctgaa aatccggatg gagatcctgc atcacaccac cgggcacatt gtaattcatc
    13441 gtcagccggg cgccgcaggt ttcctccatt atttcattga tactttctct ttcgcggaag
    13501 gcatgaaaga aaggcgtgaa tgcgcccagg tccatggcca tggcacccca ccagagttca
    13561 tgggaagcga tgcgggtcag ttcatccatt agtaccctga tcaccttggc ccgttcaggc
    13621 acttcaatct gcatcccttt ttccacgcag agcgcacagg catggttgtt aatatgggaa
    13681 gagagatagt ccatccggct ggtgacataa ataaattgcc ggtaggtcag gctctcgcac
    13741 attttttcga tcgaacggtg gatatagccc aggtggggtt ccactttcat gatagtttcc
    13801 ccgctcaggg taatcaccag gtgcagtacc ccgtgtgtag ccgggtgttg cggacccaca
    13861 ttgatggtga attcaccggt ttccccgaca atgccggggc tggtttcttt tattatttgg
    13921 gtttccctgt acatgctgtt cttgctgttt tgtcttaaag acgaatcata ttaatcgggt
    13981 cctcaaaatc ttttcgcaac gggttctttc cttcccatcc gtccggtaat atcagtaatc
    14041 gtaaatcagg atgattcaga aaattcaccc caaacatttc atatacttct ctttcatgaa
    14101 attctgcggt acgccagcat ttgcagacac tgactacttc cggtttggcc cggtccagtt
    14161 tcactttcac cactatatta tgccggaaac tggtggaatg aagatgatag accatcgtca
    14221 gatgagtctt ccagtcgatg caggtgaggc agaacatata atccagcgcc aggtgactgt
    14281 ccttacaaag cgcaccggcc aacgtgggcc attctgcggc cgtcacttcc acattcagcc
    14341 attcgccgct ttcatcaaag ctggcagagg gcagcatttc ggttatccgg aatttcaaca
    14401 gttctttgga cataattaat ctttgtctgt tctgatctgt tcacgggtca ttcccgcttt
    14461 aattttattc tgcagggaaa tcagggcatg cagcagggct tcaggccgtg gcggacaacc
    14521 ggccacatac acatccaccg ggatcacatg gtccgcacct tttactacag aataggtatt
    14581 ataaaagaat ggcccgccgc tgatcgcgca ggcccccatg gcgatcacat attttggatc
    14641 ggccatctgg tcatataatc ttttgagcac cggggccatt ttattcacaa tggttccggc
    14701 aataataatc acatcggcct ggcgtggggt agcccgggcc acttcaaatc caaaacggga
    14761 aaaatcgtat ttggcaccgg cgacagccat atattcgata ccacagcaac tggtggcgaa
    14821 ggtcagcggc caaagtgaat tggaccgggc ccagttgatg atatcatcca gtttgctgag
    14881 gacaatgcca cctcccggat tctcatgaat ctgtccgggg aaatcgttgg cggtattttg
    14941 tttcactgac atcttataat attatactag acccatttaa gggctccttt tttgtgggca
    15001 tataaaaatc cggtaaacag gataaaaaag aaaatcacta tttccaccaa tgctacccag
    15061 cccatactct tggccaccac ggcccagggg tacagaaaca ccagttccac atcaaaaatc
    15121 aggaagatca gggcaaaaag gtaataaccc acattcagct ggatccaggt cttgcccttg
    15181 gtgggtatac cacactcgta gggctcaccc ttttgtttgt ttttagtagc ttttaataca
    15241 aaagtggata gcatgatcgc gatgcctgag aaggcaactg cggcaataat cagaactata
    15301 agcgaagatg cgcccatata cagattgatt tgcacgaata tagtaacaat gcgctaaatg
    15361 cggtacatga cacatatcac cgcaaaactt gttctttgtc agttaaattt ttgggtgaag
    15421 ggcagggtta tccgtataat tcctggtgta tagttttttt atcatacccc atttccagga
    15481 ttactacttt agcctcatca atcatttgtt tccagccgca aaggaaaaaa taggcgtcct
    15541 gccgttcttt gcagagttcc tggtatactt catgcacatg cccgttatgt ccctcccagt
    15601 gttcccggga aagtaccggg tgaaaacgga atccgggcat tttttcttcc agttccatca
    15661 attcatcaaa atataaaaga tcagccttat tacggcagcc aaaaatcagg tggactttct
    15721 ggtgtggaac cttttgcaag tggatatatt gggccatact ccggaacggg gcaatgccgg
    15781 taccggtaca aatcaggaaa agttctttat cgagctgggg cggaagggaa aaaacaccct
    15841 gggcgccgcg gaaaaccagt tcactgccca cgttgatttc ttcaaataaa taggaagttc
    15901 cggaaccgcc ttccgcatgt acaatcagca attcaaatat attactgcca tcgggccagg
    15961 aggaaatgga ataactgcgc catcttttgt tgggcttctc gtgaatgggc aggtccaggg
    16021 taacaaactg gccaggcatg aaatcaaacc gctccagttc gggtacctgg atccagaaac
    16081 gacgggtatt atgggtatga tcgtcaatct tcgtaactat tccttttcgc cagggtagta
    16141 atgccatgct tggtggtttt gataaaggta aaaccaacga accattaata tgttctgaaa
    16201 cacggaaatt atcgtgcttt accccgtttg aattatcttt gtacagaaca tgagtgtcca
    16261 ggaattgttg caaaaatacc atcagacacc ccgccttttt caactggcgg acaggctctc
    16321 ttttgcccgg cccgaaaaaa tttacctgga gaatttaaga ggcagtgctt cccagtttat
    16381 cgtggctgct gcgtttacac acccttcgct ctctcagatg aaccacctgg tgatcctgaa
    16441 cgacggggaa gaagccgcct attttcacaa taccctcgag aacctgactg aagcactcga
    16501 tattttttat ttcccggcct ccttcaaaaa ccggaaaaat taccagttgc tcaattcctc
    16561 gcacgtcatg ctgcgtaccg aagccctgac aaaaatcgct gcggggggta ataaaaaagt
    16621 actggtcacc tacccggaag ccctttttga aaaagtggtt ttaccaaaaa ccctatcctc
    16681 caatattatt catatccgta ccggggatac cctggatacc ggtcagcttt tattgaaact
    16741 gggggattat ggatttgagc ggactgattt tgtttatgaa cccgggcagt ttgccatccg
    16801 cgggggaatt cttgatattt attcattcgg aaatgaaaaa ccttaccgcg tagagttata
    16861 cgggaatgaa gtggactcta tccgtatcgt ggatcccgaa acccagctca gcgagcgaaa
    16921 actgctgcag gtaagcatca ttcctaatgt ggatacacag tttgaagagg aagaccggat
    16981 ttcccttttc gaatttcttc cggaaaatac cgtgatctgg atacaggatt atgccctgtg
    17041 ccgcgaacat attgccgata gtattgaaga ccttgaatct ttcctgcgta ttcaggaaga
    17101 aatccgggcg caaccggtcg ttaaaaccaa acaccgggag gagctggatg ataaactgga
    17161 aaagaaacag gtgtcgatga aggattttat cagcatcccg gaatgggagg aaaaaatcgt
    17221 gcagcgtcac ctggtgggct ttggacatgg cgattccttc ccggatcatt tttccccgct
    17281 tacgattcgc cataacaccc gggagcagcc ttccttcaac aggcagtttg acctgttgat
    17341 ccgtgacctg aagagctggg aggcaaagaa atttcagtta tacattttcg cggagaatcc
    17401 gagacagctc gaacggatgc agagtatttt tgatgacctc aaagcggata tcgtttttaa
    17461 tcccgtgtcc acttccattc acgaaggatt tatcgacgaa gacctgcaat tactttgtta
    17521 taccgatcac caggttttcc agcgttacca taaatacaaa gtcaaacagg cgtataacaa
    17581 aaacaaggcg cttaccctac gcaccctgcg tgagttgcag ccgggtgatt tcgtgaccca
    17641 tattgaccat ggcgtgggcg tattcagcgg tctgcagaaa attgaagtga acggtcgtac
    17701 ccaggaagca gtacgactga tctataagga tacggatatt ctttatgtca atatcaacag
    17761 cctccataaa attgccaagt ataccgggaa agacggcagt gtgcccaaag taaataaact
    17821 gggtagtgat gtttggaata aactgaaaga gaaaacaaag gccaaggtca aagagatcgc
    17881 atttgacctg atcaagttgt acgccaagcg gaaagcgcag cagggatttt cgcatacgcc
    17941 tgataattac atgcagacgg aactggaagc ctcttttatt tatgaggata cccctgacca
    18001 aagcaaggcc gctgccgatg tgaaaaaaga tatggaatcc ccatctccta tggatcggct
    18061 ggtttgcggg gatgtgggct ttggaaaaac agaagtcgcg atccgtgccg ctttcaaaac
    18121 ctgcctggac ggcaaacagg cagccgtact ggtgcctact actattctgg cctttcagca
    18181 ctataaaaca ttcaaggacc ggttaaagga tttcccggtc accgtagatt atatcaaccg
    18241 gtttaaatcg gctaaagagc ggaaggaaac cctgaaaaaa ctcgaagaag gaaagatcga
    18301 tatcattata ggtacacatg gattgctggg gaaggaagta aagttcaagg acctgggctt
    18361 gctggtggtg gatgaagaac aaaaattcgg cgtgggacat aaggaaaaaa taaaaaccct
    18421 ccggactacc gtggattgtc tgacattgac ggccaccccg atcccgagaa cacttcagtt
    18481 ttcgcttatg ggtgcccgtg acctgagtat catgaatacc ccgccgccca accggcagcc
    18541 gatccagacc gaagtgcagg tatacaacga agactttatc cgcgatgcca tctatttcga
    18601 aaccgaaaga ggaggacagg tcttctttat ctataaccgg attgccggcc tggccgagat
    18661 ggccgcgatt atccaggcac tttgtcctga cctgagtatc ggctttgccc atggacaaat
    18721 ggaaggtcat gaactggaag aaaagatcct ggactttatc gacaggcgtt atgatgtact
    18781 ggtctgtacc aatattgtgg aaagcggggt ggatattccg aatgtaaata ccatcatcgt
    18841 aaacaatgcg caccagtttg gtttgagtga tctgcatcag ctacgcggac gggtgggccg
    18901 gagtaataaa aaagccttct gttatctgtt ggcaccaccc atgagtaccc ttcccaacga
    18961 ttcacggaaa cgattacaaa cacttgaaca gcacagtgaa ctcggcagtg gattccagat
    19021 cgccatgcgg gacctcgata tccgcggggc cggtaacctc ctgggcgggg aacaaagtgg
    19081 tttcatggcc gagattggtt ttgaaatgta ccagaagatc cttgatgaag cgatccgtga
    19141 attgaaacgg accgacttca aagacctctt caaggaagaa atcgcccggc aggatgattt
    19201 tgtgcaggat tgtacgatcg acaccgacct ggaaattttg atcccggatg aatacgtgga
    19261 aagtattgcc gaacggcttt ccctgtatca gcgattagat aatgcagaat ctgaagagga
    19321 actgctggcc atgaaaacgg aactcgaaga ccggttcggc acattacccc gacaggtgga
    19381 tgatctgttt attacggtcc gatgccggaa actggcagtc gacctgggct ttgaaaaaat
    19441 gagtctgaag gataataccc tccgttgttt ctttatcaac cgcccggatt ctccctattt
    19501 tgaatccggc acctttcaga aaatcataca gtttctgcag accgagacta ataaaggcaa
    19561 gctgaaacaa accggccggc ttttcctcct ggtggccaat gaccttcagg atatggcggc
    19621 gatgcaccgt tttctggcag ccatgcaccg gtattgtttt ccagcccccg ttgcaactgt
    19681 ttaaggatca gttctgcatt gtctgaattc cttgtctgta ttggtaattc tcaggtattt
    19741 gtattacttt tagatagcta aaaatcgcta tgagaatcta tccaattccc ttgattgcct
    19801 tcctgttggt tacttgtttt actgcaacag cgcagcagga tgcccgcttt gccctcttat
    19861 taaaaagcgg ggccgtattt cccaccaaaa atatcagtac cagccgggtt gactctttta
    19921 ataaccgcag ttcacgtact gccggaaagt ccttcgccat tcttcaattt gagcaattac
    19981 ccaccgcagc tgaaagacaa caattgctgc aggcaggaat tgaattactc gattatattc
    20041 ctaataacgc ctataccgca acgattaccg gtttactcag tgaagcaacc ctgcagcgcg
    20101 tacaggcaag ggccattata gagccatcgc cccagcagaa aatgcagccg caactcgcca
    20161 ggggtttatt cccaacatat gcgattaaaa cacccggtac catcgacctg tggatcagtt
    20221 atccgaaatc ttttttaccc gaacaagtga aagaggaatt gcgtcggggt aattatgact
    20281 tagttagtaa cgattatcag ttgtaccgga ttctcggcgt acgcattgct gccagccggc
    20341 tggaagaact ggccgctacc ccctgggtgg aatatgtgca ggccattccc gcccctgatc
    20401 gtgagttaaa cagtaacagc atgtatacca ccagagctag tgtgctgaga gctccattgt
    20461 cagccggtgg acgtaatctg gatggacagg gtgttgtagt gggtgtcgga gataatggtg
    20521 acatacaatc ccatcttgat tttaatggaa ggttgatcaa ccgggcggct gatatcatgc
    20581 gggcgcatgc cacgcacgta gcaggtacga tcggtggtgc cggtatcata caggaattat
    20641 ataccggcta tgcaccaaag gcaacattac ttacacaata ctattccggc atctttacag
    20701 aagccccggc ctatgtacag gatcatggta tggtgatcac caataactct tttggcgcgg
    20761 tggtggacga ttgtttcttt aatggtttat acgatctcac gtcccgtgta ctcgatcagc
    20821 aggccattga tcttcccgaa ttgcaacagg tatttgctgc cggcaataat ggagccttaa
    20881 cctgctcgcc gtatcctgcc ggatttaaaa ccgtattggg aggctatcaa tcggccaaga
    20941 atgtactcac agtgggaagc accgatttcc ggagcaatat cgcggcttct tccagtcgcg
    21001 gtccggtaaa ggatggccgt gtaaaaccgg agattatgtc acagggagta tttctcgcct
    21061 ccacctggac cagcaatatc tatagttata acaacggtac cagtatggct gcaccgggag
    21121 tttccggcgg actcgcatta ctcgtacaac gatatcggca attaaatgcc ggggctaatc
    21181 cgaagaacgg attaagtaaa gcgatactgg tcaacggggc agatgataaa ggaaacagcg
    21241 ggcccgattt ccgatatggt tttggtcggc tcaacctggt tcgatcgctg gccatgatag
    21301 aaggcaacac gtattttaat tcaaccgtgg ccaataccgt aaccaataca catacgatct
    21361 cggtgccggc caatacggct aaactcaaag tactggttta ctggcaggat cccccggcag
    21421 cagtaatggc cagcaaaaca ttagtcaatg atattgacct ggaagtaaat acacccgcca
    21481 gttttacggt gttcccttcc gtattggata ctttacctgc taacgtgaat aacctggcag
    21541 caagcggtac cgatcatatc aacaatattg aacaggtgga aattaataat cccacagccg
    21601 gtaactacga tttgaaagta atcggaaatg cgatcaccca gaacccatca caggagtatt
    21661 tcctggtata cgatatcata ccaagaagcc ttgtgctgac caatcctgtt gggggtgata
    21721 aattcacacc aagcgttact actctcgata ccgtgtatat tcaatgggat gattatggca
    21781 gcagtggtac ctatacactc gaattttcac ctgataacgg cagcagctgg attttgctga
    21841 ataacgccgt ttcagccagc agccgggtat atagctgggg cgtacccaat acccccactg
    21901 atcagggccg gatccggatt accaacaata ccagtggcct gacccaaacc agtgctgctt
    21961 ttacgatagc caccctgaat accgtctcac tcgatgcggt gcaatgcgaa ggatatataa
    22021 aaataaactg gaccgccgtt cccggtgcta cggattatga agtaatgtgg ctgcaaggaa
    22081 ctgaaatggt ttcagtgacg actactgctg cattgaccta tactttcagt ggtttgtcaa
    22141 aagattccac gtattgggtt actgtccgtc cgcggatcaa tggcaatccc gggcgacgtg
    22201 ccaatgccat cagtcgccaa cccaatgcag gtacctgtgc gggaactatt tcggataacg
    22261 acctgaaact ggatgcgatt gtttcccccg ccacttccgg acgtgaactg accagcaccg
    22321 ccctgagcgc cactcatgcg gttaccatac ggattaaaaa ccttgatgat gccatttcat
    22381 ctggtaatat caatgtgagt tattccatca atggcggacc ggctgtcaat gaagtaatca
    22441 caactcccat agcggatatt gcagccggag gaacgctttc gcatacattc gccaccaccg
    22501 ccaatttggg agccaccgga acctatagta ttgaggtcat cgccaccaaa gcctcggatc
    22561 cggtgacggc gaataataca cagacgaaag tgtttaagca actcgccaat gccgccatta
    22621 ataatgccct gcttccctgg ctcgacgata tggaaaccgc ggcagaacaa acgatcacgg
    22681 tggaccagat gggactgacc ggcaatgacc ggtatgactt tgtcaacaat ggaatatatg
    22741 gtcaggcgaa aacatttatc cataccggca ttgcttattc cggttccaaa gcactgacgc
    22801 ttgatcagag tcgttttgta tccggcggaa gtgttgattc actcaccggt acctttaacc
    22861 tggccacctt caatacggca acggatgata taaggatcga tttccggtat aaaaatcacg
    22921 ggcaaaaatc caatgccgcc aataaagtat ggatacgggg taatgataca gacaactgga
    22981 tagcggtcta tgacctgtat gccaatcagc cagctgcgga tggttccttt aaactctccg
    23041 gcagtattga attaagtgat tcactggccg cttatggaca atccttcagt accagcacac
    23101 aagttcgttg gggacaatgg ggcgagtatc tggcggctga taatttcagg gcggcaggat
    23161 atacgtttga tgatatccgt ctttataaag cggttgatga tatccaactg gtcagtgtgg
    23221 atacgcctta tacggtaagt tgcggactca gtgcagcggt gccggtaaaa gtaacggtac
    23281 ggaatacgtc gaatgccatc atcaataata ttcccatcac gctgaaagtg gatggagtgg
    23341 tcatcgccaa tgaaacgatt acttccattg cagccagctc atcggttcag tacacgttta
    23401 ccgcaacggc caatcttacc accagtggta atcatattat agaggtatgg tcggccctgg
    23461 caacggatac ctataatcag aatgacacgg tcagcagcac ggtgaataat ctgcccctgg
    23521 tcagcagctt cccttacctg cagaattttg aaaccaataa tggttcctgg tataccggag
    23581 gcatcttatc ctcctgggaa tatggtacgc cgatctctcc gaagatcaac cgggctgcca
    23641 gtggcagcaa ggcctggaag acaaggatcg ccggctacta taatgacaat gaattttctt
    23701 atctctattc gccctgcttt gatatttcaa ccatgaccaa tcctaccctc agtttcagcg
    23761 tggccatgga catcgaagat tgcggcgcgg tcctttgtga tgccgcctgg gtggaatatt
    23821 ctgaagatgg aattacctgg acaaaactgg gcagctttgg acaaggaacg aattggtaca
    23881 ataaaaacta tgcgggaaat cagttatgga gccagcaaaa ttataccaac tggcatgtag
    23941 ccacaacggc tttacccacc agcaataaca gccggctccg ttttcgattt gtgtttaatt
    24001 ccgactcagg gctgaataaa gacgggatag cggtggatga tattcatatt tacgataacc
    24061 tgtacggttt atatgatggt gcaaccatgg cagccccggt aacacagacc atcaataccg
    24121 gtaccaatac ctgggtggat ttcctggaag gaggtaaact ggtggcttcg gttcatccgg
    24181 ccggacagga aatgggagct actgatgtta gagcctatct gcataccggt gcggtacgct
    24241 ggagcagtaa ccagtattat cataaccgca actttaccat tcagcctgcc aacgcgttcc
    24301 tggcactggg tgattcggtg ttggtgcggg tgtatttccc ggataccgaa acagaaaccc
    24361 tgattgcggc tacaggttgc agtggttgtg ccaaaccggc tacggtttat gatctcgggg
    24421 taactaagta tagtgacccg gataatagtt atgaaaacgg aactattgcg gataataacc
    24481 agggtgtctg gtcttttgtt atcccgtccc gggccaccaa agtacctttc gataaaggct
    24541 attatgctga gtttaaagtc aataattttt ccgagttctg gctcaatacc ggcggactta
    24601 caggcagtca gcctttgcct gttgagttga agcagtttac tgttcgtaaa ctgccccagg
    24661 atgatgtact ggctgaatgg gtgactgctt cggaatacaa tgtaaaccgg tttgagattg
    24721 aagtggcccg tggcaatgcc gattaccagt cgggccgtta tacacgtttg ggtgaaatac
    24781 tgagccaggg taattcagac cgggaacaac gctaccagta tctggacaca gagcccggaa
    24841 aatcaggggt acgctattac cggttgaaaa taatcgataa tgacggaacg gtgcgctact
    24901 cagcgatccg cccgctggta ttcaatattg aaattcaatg gaccgttaca cccaatccat
    24961 ccgccggcca gttcagcctc aactgccagg ccagtccggg tgaaacgatc caggtgaaag
    25021 tctatgatac aaagggccgc ctcgtttacc agcacagcaa cccggccagc ggctttgccc
    25081 aaaaactgaa cattgacctg aaaggcccgg cctacccggc gggaatttat ttgctggaaa
    25141 caggagtggg agagaagagg aggagcttta aactggtgaa acaatgagta atcagtaaag
    25201 ttggtagatg cttattttct tggacctgtt ttaaagagga aagggcaatg aaaaccattt
    25261 aataagaagc taatttatag aatagtaaaa cgacagagcc ctccgaaaac cggagggctc
    25321 tgttattaag gactgtttaa tcagctactc attactgatt attcattatt cttaccagcc
    25381 ttgcttggct ataccggctt taatcgccgc cagcgctttt tcttcgccca tcagggtggt
    25441 cagtttattt cctttaccat catttccctg ggccatatag gctccttttg cggtcttcgt
    25501 gataacggca tccagcattt ccacaccttt ctccttggtt tttacattgt aggctgtaat
    25561 ttttacatct gacatggttt aagtttttgc gttagtaaag gatgaaatta cgcaatccgg
    25621 ggggttcaag gggagtaaat ttggctttta atgaaaaaag gctccgaaaa atcggagcca
    25681 agttgcttat agtcaatatt ttataaattc aaagtccaat ttgttaaaac cagccgggaa
    25741 gcccgcagaa ccggttaaat atccaatttt gcatatttcg catgactttc aatgaattcc
    25801 ctccggggag ctacttcatc gcccatcagc atactgaaca cccggtctgc ctccgcagcg
    25861 ctttcgatgg tcacccgctt cagggtgcgg gtttcggggt tcatcgtggt ttcccaaagc
    25921 tgggaagcat tcatctcacc cagacctttg taacgctgga tgtttacgga gtcgtctttg
    25981 ccgccgccca gtttttccac ccataactta cgttggtctt cgttgtaagc atatgcctgt
    26041 tccttgcctt tcttcaccag gtacagcggg ggttgggcaa tgtacacata gccctgctct
    26101 accagttcgg tcatatacct gaatacgaaa gtcaggatca gggtggcgat atgggatccg
    26161 tccacgtcgg catcggtcat gatgatcagc ttatgataac ggagacgact caggtcgagc
    26221 gccttcggat cttccggggt tcccatcttc acgcccaggg cggtaaacat gttccggatc
    26281 tcctcgttgt catagatctt atgttccatg gctttctcca cgttcaggat cttaccacgg
    26341 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcccggcg gaaaccggat
    26461 acgtgggtac caccttcaat ggtgttgata ttattgacgt aagaatagat atgttcattg
    26521 tagctgtcgt tatagttcat ggccacttca acggctacat tattggcttc atcccgtcct
    26581 tccactgaaa ttactttcgg aatcagggta ttacgaccgg cattggcatc cagcatttcc
    26641 acaaactcca caataccgcc ttcgctgtaa aactctttgc tgtaattact gccgtcttca
    26701 tttttctccc gcaggtcagt gaggatgatg cgtacaccac ggttcaggta agccagttca
    26761 cggaggcggc tttccaggat atccttattg taagtggtag tgataaagat gctgtcatcg
    26821 ggccagaaat gaacccgggt accggtggtt tcactggtac ctgtttcacg tacggcgtat
    26881 tgaggcaccc cgatacgata ttcctgttca aatatctttc catcacgatt tacggtaacc
    26941 agcagttttg aactaagggc attcacacaa ctcaccccca caccatgcag accacccgat
    27001 actttgtaag aacctttatc aaacttaccg cccgcgtgaa gcacggtcat taccacttcc
    27061 agggctgatt ttttttcctt gctgtgcatg gcagtcggga taccacgtcc atcatcttcc
    27121 acggcaattg aattgtcctc gtgtatcgcg acattgatat ttttgcaata accggccagg
    27181 gcttcatcga tggagttgtc cactacttca tatacgaggt gatgcaaacc ttttacgccg
    27241 atatcgccaa tatacatggc gggccgcttc cggacggctt ccaatccttc cagaacctgt
    27301 atactatctg cgctgtaacc ggctgaaatc tgggccgctt tttcttgtgc ttccgtggtc
    27361 ataattgagt ataaaatcgg gcgagtgtga ctgaaaaaat ataactcaca aatgtacgaa
    27421 atcggggtgg ggtaatggga tttattctgt tgaattttgc ccaaaaaaag ggggcattgt
    27481 ggataattga aaagcctgaa aatagaaggg aaaaaactgt caggtgtcgg gtgtcaggtg
    27541 tccggtgtca ggaaacagcc cctgctgtga acatatttca gcgcttttgg ggtaaactgc
    27601 tatttcgagc gtagcgagaa atcttgtatt tcctttttct ccccccataa cttgtcattc
    27661 cgcagcgagt agcgagcttg tctcgctgcg aagcaaggaa ccttgtatta aacaggttta
    27721 gctgtttaat acaactcaaa aatggtcaaa ttctttctgt atctccaacc cccaacctcg
    27781 aacctccaac ctcgaacctc caacctccta ccgcttcaca atctgcgcta ccaggtcggc
    27841 ttcacagcag actttattgc ccacataggt cgttcctttc atctcgcaga taccgcgtcg
    27901 gatgggggct gccagctcca ttttcaggat cagggtatcg cccgggcgga ccatctgttt
    27961 gaatttacag ttatcgattt tcaggaaata agtgtcgtat tgaccatccc cgctgaggtt
    28021 gatggccagg atgccgccgc actgggccag ggcttcgatc tgcaatacac cgggcataat
    28081 gggattgccg ggaaaatgac cctggaaaaa ccactcgttg aaagtgacat tttttacccc
    28141 cacaatatgc gtatcgctga gttcgatcac tttgtctaca aaggcaaagg gaaaacggtg
    28201 gggcagtgtt ttctcaatat actgcaggtc atatacgggg ggctgggaag gatcatagac
    28261 cgggatgccc tgcgtgtgtt tgtttttttt tatatactgt ttgatcttcc gggcaaagtc
    28321 cacattgctg ctatgtccgg gacggttggc aatgatcctt gctttgatgg gatagccgat
    28381 cagggccaga tcgccgatca catccagtaa tttatgccgg gccggttcat tgggaaaacg
    28441 gagttccagg ttattgaggt agccttcgct ttt
//
LOCUS       DAOPMW010000008        27555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1521_length_27555_cov_12.796727, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000008 DAOPMW010000000
VERSION     DAOPMW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 27555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_27555_cov_12.796727"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PLU11_00940"
     CDS             complement(<1..1625)
                     /locus_tag="PLU11_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPN57698.1"
                     /translation="MKRTLSLLISILLLTPVCMWSQNRFDVIISELMPDPSPTVGLPA
                     SEWIELKNCSSQPVSLQNWRITDANGQSGPLPSFVLQPDSFLIICSSSALAAMSQSGT
                     AIAVSSFPSLDNEGEWIALKAPDGRVIHSVNYTSNWYGNALKQEGGWSLEMIDTRNPC
                     SGKSNWTASIHPAGGSPGKKNSTEASNPDTTAPRLLRSYSQDSVTIRLVFDEPLDSAT
                     AVSSSQYSISQGITISSVSTVTPLFQEVQLKLNSPLQHNLVYTVKADGISDCKGNRLT
                     VTETKAGRAQDPVPGQIVVNEILFNPAPNGEDYVEIYNHSNQVADLSRLFMGNRNSSG
                     SVSSIKALSSVPFYLYPGEYLAFTESPDQLPLFFMVRFPDRVLAITSPPSFPDEEGTV
                     LLLNGQGVVIDEVKYNKDWHFKLISNDEGVALERLDPGRASGDPGNWHSAATTAGYGT
                     PGYQNSQYRSADMGAGQIKVSPAVFSPDNDGRDDLALIQYSLEESGYVANLVIFDAEG
                     KPVKQLVRNALLGRTGSWTWDGLDDSRRSLPLG"
     gene            1930..2940
                     /locus_tag="PLU11_00945"
     CDS             1930..2940
                     /locus_tag="PLU11_00945"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPN57699.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTELVPVASEKSVGKEVE
                     FKGKKYKVVSMSDAIAAKPAVAIFSAGGGTSLEWAPKFAEAGITVVDNSSAWRMDPSK
                     PLVVPEINADILTVNDKIIANPNCSTIQMVVALNPLHKKYGIKRIVVSTYQSVTGTGV
                     KAVEQLHGERRKAVKGETTDYPMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVKETNK
                     IMRDDSIRVTATTVRIPVMGGHSEAVNVEFANDYDLAEVKALLAASPGVVVVDDLANQ
                     QYPMPMDAHEKDEVFVGRLRRDESQPNTLNMWVVSDNLRKGAATNAVQIAEYLNSKGL
                     LG"
     gene            complement(2929..3597)
                     /locus_tag="PLU11_00950"
     CDS             complement(2929..3597)
                     /locus_tag="PLU11_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPN57700.1"
                     /translation="MLQPATLNFLKALKKNNNKEWFEKNRKTYEAAKADYTDFVQKVI
                     NEFGKKNPAYSSMLAKNCLFRINRDVRFSKDKSPYKTNMGAYFNPGGKKATTAGFYFH
                     CEPGQAFMGGGLWMPMPPQLSKIRQEIDYNYADFKKILANKKFKAVYGDLSRSAAYTL
                     VRLPKGYEADNPAGDYLKLKSFVALSPMKDTDLSSKDLVKKTVAAFEALQPLIHFINH
                     ALAE"
     gene            complement(3822..4382)
                     /locus_tag="PLU11_00955"
     CDS             complement(3822..4382)
                     /locus_tag="PLU11_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HPN57701.1"
                     /translation="MIKKLAGEVWKPLSFSGWRQLRNRYAVSSKGRIASYKEDITDGK
                     LLSGSLTTGYRTLNLHRPDNKGTLYIHREIAKLMVDRKSAAHKYVIHKNHNKLDNDVK
                     NLKWANLEEMMAHQQNSPAKKAYKIKQANKTTGLKLTASQVSKIKATLASKKRTLSIR
                     QLAEKYGVSEMTLYRIKSGENWGRVK"
     gene            complement(4498..6600)
                     /gene="ligA"
                     /locus_tag="PLU11_00960"
     CDS             complement(4498..6600)
                     /gene="ligA"
                     /locus_tag="PLU11_00960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPN57702.1"
                     /translation="MYSAEHTKATQHLTNELISLLKTGSIPASRVEDLREVLRFHEYR
                     YYVLTEPLIADFEYDQLYKALEKIEQEEPILVTADSPTQRVAKGLTKDFPTVQHLVPM
                     LSLDNSYNAEDLQDFDRKARELTGLDEIEYCVEPKFDGASISLIFENDLLVRGATRGD
                     GVEGDEITNNIRQIKTIPLSAAFSRQGIQQVEIRGEVLINKSNFKKYNDQLIALGEDP
                     KANPRNMASGTLRLKDSAEVAKRNLEAFVYHVSYYTLQKEAALPVNLTTHAGSLKLLW
                     ELGFRSPEQEKKVFKGIDAVIRYVEEFEATRDDLPYEIDGMVIKVNDFALQDKMGMTS
                     HHPRWAIAFKFKARQATSKLLNVEFQVGRTGAVTPVAKLEPVAVGGVTVSSISVHNEE
                     YIREKDLRIGDAVLIERAGDVIPQIVKSMPELRNGQEQPIQFPKQCPVCASQLFKEEE
                     EAVWRCTNIECPAQVVERIIHFVSKDAMDIRGFGEANVRKFYEMGLLKDIPSIYILDF
                     NKLKGLEGFGQKSIDNLQSAITNSKKQPLHRLIYALGIRFVGETTAKTLAQAVNHLLD
                     FKGFSLEQLQALEDVGPKVGGSIFQFFSNPDNITMLLELEQLGLTLKNEKKEMSSTGS
                     LTGQTFLFTGTLPTLKRSQAEAMAEEQGGQILGGVSAKLNYLVVGEDAGSKLEKAKKI
                     NSVKIISEDEFLQLIGKK"
     gene            6775..9687
                     /locus_tag="PLU11_00965"
     CDS             6775..9687
                     /locus_tag="PLU11_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN57703.1"
                     /translation="MKRTIPLLIALVFFVVKANAQLLTWTPDFAKDNDNIVITMDASK
                     GNQGLLNYSTTSDVYVHIGLITSASTSSSDWKYAKFTWATTPATAQATYIGNNKWTYT
                     INNPRTFFNSPAGVPSGETIQKIAILFRNGAGTVVQRNADASDMFIPVYDNTLATRFT
                     VPFFQPTSTRIPEPITKQLGDNIQLTAIANISSTMKLFLNGTEIQTASGVTTISANPV
                     LTTGGNQTIRVDAIDGATTKSESFSFFVSTPVNIAPLPAGVRDGINYEAGNTSAVLVL
                     YAPNKTRVSVIGEFPGSNWAEQSNYVMNKTPDGNYWWLRVSGLTAGTEYAFQYLVDGS
                     LKVGDPYAEKILDPNNDNFITSTVYPSLKAYPTGLTTGIVSVLQTNAPAYAWTVNNFA
                     RPDKRNLVIYEMLLRDFIEAHDWKTLRDTLNYFQTMGVNAIQLMPINEFDGNESWGYN
                     PSYFLAPDKYYGPKNRLKEFIDSCHRRGIAVIMDIALNHTTGDNPLAKLYWNSSTNQP
                     AANNPWLNVSATHPYNVFNDFNHESLATRYFTSRVVEHWLQEYKVDGFRFDLSKGFTQ
                     NAQCGSSTTNESCISQYHADRVAIWKRYYDTCQLKAAGSYMILEHFAADNEEIELSDY
                     GMMLWGNDTYNFNEASMGWVNTSNFERYLYNARGWSKPHLVSYMESHDEERLMYKNLN
                     FGNNSNSAHNVRDLATALRRIELCAGFFLAAPGPKMVWQFGEQGYDFSINRCTDGTIN
                     TNCRLSNKPIRWDYLTVTGRKRLYDIFSSMNKLRAHPWYKDVFTANNITINRSLAGAF
                     KTLTLRSATDTSMLCVIGNFDVNAQTGSFTFPNAGTWYDYLGGGTFTSTGAAQSFTLQ
                     PGELHVFVNRNLTNAVVTADTSVTPAGNRFAAQLYPNPGVAASQLEIEMPQTGLVRAE
                     IADISGRKIGTLLSQSLAKGKHRIAIGEKISMLPAGIYIVSIRTGTKTIQLKLAIQ"
     gene            9707..10438
                     /locus_tag="PLU11_00970"
     CDS             9707..10438
                     /locus_tag="PLU11_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPN57704.1"
                     /translation="MTEKALQQLKTAETEKISHLDYFNIGISIDCVILGYEEKELKVL
                     LIKSDMQEFSGLYSLLGDFIKPDEDLEAASYRVLKERTGLDDVFLEQVKTFGNIYRHP
                     SGRVITVAYYSLIDVSNHKLQLDLNELHWHKVDEINQLAFDHKLILDTCLGRLREQVM
                     EHPVIFNLLPEKFSLRELQELYEAILGVELDRRNFRKKISIKDWLVDIDEMENDVPHR
                     PGKLYMLKKQYRNKAIKATMPLQLA"
     gene            complement(10440..11435)
                     /locus_tag="PLU11_00975"
     CDS             complement(10440..11435)
                     /locus_tag="PLU11_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN57705.1"
                     /translation="MGSPATLKKISTQLNISISTVSRALKNHPDISENTKRKVKELAL
                     LMEYEPNSYAVNLRTNKSRVFGLIVPVISNLFYDSFIAAVEEEARRCGYSLIILQSGD
                     DPLQESENLKLCKLNRVDGVFVSLVADSNSAPLYNKLKESGTPVVFFDKVPDDAGFDT
                     ICMADEEAATMAANTILKYKKKKVLALLGNADLSITRKRKDAFLKVFEKEKSAPSLDI
                     RHCLNSAEARKITTEVLGKKNRPDHIFCMSDELLIGAMKSIYQTDLHIPDDLSVLAIS
                     NGFIPGLYKPEITYIETSGFELGKLSVKRMLEIMEGPSPSRSIILPSRLVTGGSL"
     gene            11775..14750
                     /locus_tag="PLU11_00980"
     CDS             11775..14750
                     /locus_tag="PLU11_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN57706.1"
                     /translation="MSAKTLLSAILCLVFAVSVQVVSAQDRTVTGKVTDSKDGSPVSG
                     ASVQPKGSKTGTSTKPDGTFSLNVGAGVSELVITSIGYETQTVSVSGKNSVDVSFVAS
                     AGSSLNEVVVTGYGTTRKKDLTGSVSSVKAKDFNKGTVTSPDQLIQGKVAGLQIVNNS
                     GQPGGGATIRIRGTSSVRSGNNPLIVVDGVPLDGGTARPGLGVGGVGTSPASNPLNFI
                     NPSEIASMDVLKDASATAIYGSRGANGVIIITTKRGQSGAPQIDVNYTAGTSSIMKKL
                     DVLTGDEYRAALTQYGLTSGNYGGSVNAMDEIMRKGMTQNISVAASGGNENAKYRFAA
                     SALNEEGIVKESGLKKYTIDFKGNFKLLESKKLGLDVSLMTSQVKESVVPITTDVGFE
                     GNLIGQALQWNPTHPMMKTDGTFWSEPQFGATSINPLWFLKMYDDQVNTSTVLASISP
                     YYKFSNSLEYRFLYSFNESNGSRKAQIGRQLTNIPAFKDRGWASVSAGKLRTQQFTHT
                     LNFNKQLSNALNLSAVAGYEYTKFEFANNGQTAQDFIDIGIPYYNMLGYATQASRAIW
                     NFENPTNELQSYFARTSFNLRDKYLLTATLRADGSTKFGENNKYGYFPSLAAAWNISS
                     ESFMQGVDFVSNLKLRLGWGRTGNQEFPAGSSQTRFGVTGIGSISQLNVGNPDLKWET
                     SSTTNIGLDFALLNRKVNVSVDYFNKLTEDVLFELSFPVPGGSSARIWRNLPATIKNN
                     GIEIAVNTNLVSKTDFSWNLGVNATFLKNMLEGLNGTYNTGALHGQGSTGAFVQKLAS
                     GYPLNVYYLREFMGIDKTTGQSIYQEDGDVSYYGKDANPRTLLGLSSEMSYKKFGLVI
                     NMNGAMGHYLYNNTAQSVVPITNLGSRNVASSLISASVKEDLSNPITSSTRFLEKGNY
                     LKMANMTLSYDLGQFAKFVKGARVSLTGQNLFVITNFTGFDPEVNVDKNIGGIPSNGI
                     EYIPYPTARRIQLNFNFSL"
     gene            14775..16055
                     /locus_tag="PLU11_00985"
     CDS             14775..16055
                     /locus_tag="PLU11_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN57707.1"
                     /translation="MKYGKVILVASAVLSLTACTKLDEKLTSETTSGNTGGSGSVNVS
                     ALLVGTYRSFEGLYMDQARIWAAQEHSSDEAVGPTRGGDWDDNGIWRVYHNHKWTADH
                     DFLTATFRQLAQSQFAATTVLDNNPSAQQAAEAKFLRAFAMWPVLDCWDQVPFRPTST
                     GDLTTTLPEVKKGTEAADFIISELNAAIPNLPAGRGLGIASKDAARALLMKVYLNKGV
                     YANRENPVFAPADMNQVIALADQILAGSYSLGNNFFDNFAPNNDVIGSELIYTLKADN
                     GAGVGGNGAMYSYMCGLHYNTNPSGWNGFATLGDFYDKFEATDKRRGGTYAGLTDVSG
                     LKSGFLIGQQFNQNGTALQDRKGNPLAFTREVKLKETGNNLEVTGIRVIKYIPDYAHQ
                     FPADNDMVIFRLADVLLMKAEAILRGGTATNVGP"
     gene            16110..16331
                     /locus_tag="PLU11_00990"
     CDS             16110..16331
                     /locus_tag="PLU11_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN57708.1"
                     /translation="MAALGTVDLNAVYDERGRELYWEGWRRNDMIRFGKFLAPRELKP
                     ETSGVERLLYPIPNEQLTINPNLTQNPGY"
     gene            16483..19785
                     /locus_tag="PLU11_00995"
     CDS             16483..19785
                     /locus_tag="PLU11_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPN57709.1"
                     /translation="MRKLICGLSVCLTLLAGCGDSGDEKNLFSLQQNTGIRFRNEVSN
                     SPDFNIFSYRNFYNGGGVAIGDINNDGLADVFFTANMGSNKLFLNKGDWQFDDISAKA
                     GFVNKKDWSTGVVMVDINQDGWLDIFVCNAGYINGAKPRCQLFINNKNLSFTDKAEEY
                     GLTNEGGYTTHAAFFDYDADGDLDCFIINNSFIPVNTLNYVNKRNLRAADWPVADFLK
                     GGGDHLLRNDAGKFTDVSKEAGIYGSLVSFGLGVTVGDLNGDHYPDVYVSNDFFERDY
                     LYINQRDGTFKDELEQWVQHVSHSSMGADMADVNNDGYPDIFTTDMLPDDDYRLKTTT
                     TFDNIDVHRLKQKSGFYNQYTQNTLQINNRNGKFMETGFYSGVAASDWSWGGLIFDAD
                     NDGRSDIFVCNGIYHDVTDQDFIDFFANDIIQRMVMTGEKDKFDEIVNKMPSRPIPNK
                     LFLNQGDLRFADPGNKSGLGTPSFSNGSAYGDLDNDGDLDLVVNNMNMDAFVYRNNSR
                     EINQHHYIGILLKDTGRNTFAVGSKIQVYQGTQVFSREVIPSRGFQSSVDYRQIIGLG
                     NGVADSMLITWPDGSSSRIILPEINKVHVITAAATAQHTLTASVASQPFFALTDFPVF
                     DKHTEDDFIDFYNERNIPVMLSREGPHADTADVNGDGLCDLYIGGAAGQPGQLYLQTV
                     TGSFLKTVSPAFEADKAFEEVAVLFFDADNDGDKDLFIGAGGNAQQPDSRLLAHRLYL
                     NDGKGNFANSPVKLPANTMNIAVAVAHDVDRDGDQDLFIGARSVPMQYGIVPDSYLWI
                     NNGSGAFTAGSPLKAGMITDAVFADLTGDQQAELLLVGEWMSPRLYTIQNQQLTEVKT
                     DLSSLKGWWQSVAVADLDGDKRMDIVLGNTGENFYLKPTPEQPVRLWISDFDENGTAD
                     KIITRTVNKKDMPVFLKRDLTDQLPVLKKQNLKFEAYGNKTIQQLFPGEVMQKAQMYE
                     FNSGASCMAYNEGDGRFLIQQLPVPVQFSSVNAIVCNDFNQDGRPDLVLGGNQFHFQP
                     QFSRLDASYGHFLINKGRSQWEWVAPRQSGLELRGMVRDIRLLPITGRTGLLFLQNDE
                     SPVFYTFSGAIK"
     gene            19795..23139
                     /locus_tag="PLU11_01000"
     CDS             19795..23139
                     /locus_tag="PLU11_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPN57710.1"
                     /translation="MFRQPAHILIVWCTLVTGLFSCKDTEPAGPVLFEALDHTRTGLQ
                     VSNQLTARPDFNMLKYMYFYNGAGVGTGDFNKDGLTDLFFASNQSQNRLYLNQGKLKF
                     TDVTTQAGIPDDGGWTTGISVADVNDDGWLDIYVSRVGQYETLKSKNQLLICKGLDKE
                     GIPLYQDEAAAYGLDFSGFSTQAAFFDYDLDGDLDMFLLNHSLRYNSTFAPRSNFTGT
                     VDSLSRDILYRNDNGKFSDVSTAAGIHQSVIGYGLGIVISDLNLDGWPDIYIGNDFHE
                     NDYLYINDQQGGFREELTERTMHTSQFSMGVDAGDINNDGWPDIIAADMLPSDPYILR
                     RSLGEDEYATFQIKIRNQYNHQYARNNLQLSRRNGLYSEIGLYAGVSATDWSWATLWM
                     DFDNDGRKDLFVSNGIPKRLNDIDYVNHVSNDEIQAKIRSGNMQDKDMALIDKFPEIR
                     LKNRFFLNKGQASFEDAGNRIGSNPETFSNGAVYADLDNDGDLDIVVNNIDEPAVVYR
                     NTSNDNGEHPSLQLQLNGPAGNRQAVGARLIAYSGNQVFNFEKFQARGFQSSMETPLF
                     VALGNQKPDAAWLIWPDNTCEPVSLNIDTAQIKTLTYQPGLPRFDYARLRSFYAATEV
                     PFEDITRQTGLTFMHRENVFNEFDREPLMPFMVSREGPAIAVADINQDGLEDLFVGSV
                     KWEQGAVFIQNKNATFTRVPQPALAADSTFEDTDACWTDVNKDGYPDLVVASGGNEYY
                     GQTPYLLSRVYLNDGKGHLSKKEDAWPKELMISASAVLPLDFNQDGYMDLFIGGRSVP
                     WQYGAIPASALLQNDGKGRFFNVTEKIAPQLATAGLVKDAVIADVNNDGRTDLVLALE
                     WEGIVAFLYKEGQFTRQNIVADKGWWNFILPADIDNDGDIDFIAGNQGLNSRFTVSKT
                     QPVRLYYNDFDDNGRKDQVLTYYLQGQEIPFANKAELERQMPGLKKKYLYAEEFAKAG
                     IKDMFSKQKLDQATLLTADYFSNALLINEGKGVFKTKALPWEAQLTSFRGACMADLNG
                     DNRLDVILGGNFYPNNIQMGRYDADYGSVLMNKGHGDFQVQQSTLPVKGEVRQVAPIR
                     LSGNGTAYILARNDDSLMVIRSASNR"
     gene            complement(23136..24041)
                     /locus_tag="PLU11_01005"
     CDS             complement(23136..24041)
                     /locus_tag="PLU11_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57711.1"
                     /translation="MKNRKSRLFLLTVLAVLLLATAFFGFSKQTFREQVEINYAILKT
                     GEQLRSKDQVQRWYLSSSAESDSLSVSEQTPFSARITARFQGKEKAFRFSINGDTLNA
                     QLSNVTLSYENTYFGHWFGKNPLIRDARKSLQNLKAYMEDSKRFYGYEIQLVKVEDTA
                     FLFKSVSVPLATKREATRKLFDELIAYAASKDAGYNGVRIFYSLKGEKDIKLFASIGV
                     TNAMDPDPATGIEYKKMPLGKNLLVANYQGTLNEVDKAYRALDRFKSDHNFTSMAIPF
                     QKILSDGYDFSDEQIVQLKIFSPVF"
     gene            24219..25706
                     /locus_tag="PLU11_01010"
     CDS             24219..25706
                     /locus_tag="PLU11_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN57712.1"
                     /translation="MTLAKPRLSFWQIINMNVGFFGIQYSFGLQQSAVNPIYDFLGAK
                     TDEIPWLNLAGPLTGLVIQPIIGALSDKTWHPRFGRRTPYFFIGAIICSIALFLFPFS
                     SALWMAAGLLWILDAGNNTAMEPYRAFIADKLDATQQPTGFQAQSFFTGFGQTLANLS
                     LYAFPFIIVGTTGKLPTWVFASFFLGAVCSIVSIWWSVRTTPEIVPDEEELAQIKEKN
                     KGMPHPAIQFFLSILTTLVAYAVIVLLLIPSLFIPGLIRRLLNYVEGHATLKILFAQN
                     IEIIDAIIKMPKVMWQLALVYLFQWYALFCYWQNSSKSIALSVWNTTPEENSKLYEKA
                     VGWTGLVNGWYNIVTFLCAFGLVWFARRYSPKLVHFGCLILAGIGLLAFPFIENKYLL
                     FPAITGFGIGWASMMGIPYLIVVSKIPKERYGVYMGVINMMIVIPMFIQTLSFGYILK
                     NFLHNNAGYAIAFAGVLLLIAAILTLRIQADDKPVNGEVNFSAGH"
     gene            25716..27479
                     /locus_tag="PLU11_01015"
     CDS             25716..27479
                     /locus_tag="PLU11_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPN57713.1"
                     /translation="MKKSQFNFVFFFLFIAAASCKLMPKEKEEPKKDGVKKTWWKEAT
                     VYQIYPRSFQDSDGDGVGDLKGIISRLDYIKSLGIDAVWLNPIYASPNKDNGYDISDY
                     ENIQPQYGSMTDFDTLLKGFHDRGIKVMMDMVLNHCSDEHKWFMEAKQSRNSPYYNYF
                     HWWPAEKGEPPYRFSIFDEKGYGWEFNKATNSYYLHYFGDFQPDLNWENPQLRQDIYK
                     MMRFWCDKGVDGFRMDALAFISKDTTWPAQLPAEYKGSWPMYYASGPHLHDYIQEMNQ
                     EVLSKYNLATVAEAMGDIPRVKKFVDEDRKELNMAYHFEAIDFGYLPGEYKMPDPNGY
                     DFARWKQVYAKWDSAFTDKGWGTMYLANHDQPRMVSRWGNDNPEYRDYSSKLLTTFIL
                     SMRATAFYYFGDEIGMSNIKFDRVDQYNDVELLTNYEQVKAKGGDLKRFLEGMKISSR
                     DNGRTPMQWDTTVNAGFGAGMPWLPVNGNYKTVNVSAQEKDPNSCLNYFRAMQKLRKE
                     NEVLVYGKFTLLDKDNPAVFAYTREWKGKKWLVLLNFKDKTTTVNTGFELSKATVKMH
                     NYTTASTNGTLQPYETVIYEL"
BASE COUNT         7319 a         6256 c         6631 g         7349 t
ORIGIN      
        1 tgcccgagcg gtaaggaacg tcggctatca tccaacccgt cccaggtcca gctgccggta
       61 cggcccaaca gggcattgcg gacgagttgc tttaccggtt taccttccgc atcaaagatg
      121 accaggttgg ctacataacc agattcctcc aatgaatatt gtatcaacgc cagatcatca
      181 cggccatcat tatccgggga gaacacagcc gggctgactt ttatttgtcc cgcccccata
      241 tcagccgatc ggtattgaga gttctgatac cccggtgtac cataaccggc cgtggttgcg
      301 gcactatgcc agttgcccgg atcaccggaa gcccttccgg gatccaatct ctccaatgcc
      361 accccttcat cattactgat taatttaaaa tgccagtcct tattgtactt cacttcatca
      421 atgactacgc cttgtccgtt caataatagc accgtgcctt cctcatccgg aaaagagggt
      481 ggtgaagtaa tggccagtac ccggtccggg aaacggacca taaaaaacag gggcaactga
      541 tcgggtgatt ccgtgaaggc gagatattca ccgggatata aataaaaagg tacggaactc
      601 agcgccttga tggagctgac tgagccactg ctgttgcggt tgcccataaa gagccgcgac
      661 aaatcggcca cctggttact atggttataa atttccacat aatcttcccc gttaggtgct
      721 ggattgaaca gaatttcatt gaccactatt tgtccgggta ccggatcctg tgcccgcccc
      781 gcttttgttt ccgtaaccgt cagccggttt cctttgcaat cactgattcc atccgctttt
      841 acggtataaa ctaaattgtg ctgcagggga ctgtttagtt tcagctgtac ttcctggaag
      901 agcggtgtaa cggtactcac actactaatc gttattccct ggctgatgga atattgagag
      961 ctactcactg ctgtcgcact gtccagtggt tcatcaaata ccagccggat cgtcacacta
     1021 tcctgggaat aagaacgcag gagccggggc gccgttgtat ccgggttgct tgcttctgtg
     1081 gagtttttct tacccggact accgccagcc ggatgtatac tggctgtcca gttgctttta
     1141 ccgctgcagg gattccgggt gtcgatcatt tccaaagacc agccgccttc ttgcttcagg
     1201 gcattgccat accagttgct ggtataatta acactatgaa tgactcttcc atccggagct
     1261 ttcagggcaa tccattcccc ttcattgtcc agcgaaggga aagaactcac tgcaatggca
     1321 gttccggatt gtgacatagc tgctaaagca gaagaagagc aaatgatcag gaagctatca
     1381 ggttgcaaaa caaaagaagg caacggaccg ctttgtccat tggcatccgt gatccgccag
     1441 ttttgtaagg atacaggttg gctgctgcag ttcttaagtt caatccattc actcgcgggc
     1501 agtccgactg ttggtgaggg atcgggcatc agttcactga taataacatc aaacctgttt
     1561 tgcgaccaca tacaaacagg tgtaagcaat aggatgctta taaggaggga taaggttctt
     1621 ttcatagggc aaatatgttt ttttttgata cggcgtgtgt aaccgtcgcc atattcatga
     1681 attttttttt ggcattattt ggcattcacg gcttgcggga ctatttttgc ttcgcaatga
     1741 tgacaacgaa acatacaaaa cgcattaaga aacattcctg agggaggttt gcagcgttat
     1801 gtgtgtccaa cataaaagtt tagcaggcct tccttacgga gggccttttt tttatccgtt
     1861 gtcattgtag catcaacaaa attttttaaa aaaaaacttc cgccttgcgg aagatggggg
     1921 gacaaaaaaa tgaaagttgc agtagtcggc gccacaggac ttgtgggcac caaaatgtta
     1981 caggtattgg cagaacggaa ttttccggtg accgaactgg taccggtggc ttctgaaaaa
     2041 tcagtaggga aggaagtgga gtttaaagga aagaagtata aggtagtgag tatgagcgat
     2101 gccattgccg ctaaaccggc tgttgcgatt ttctctgctg gcggtggcac ttccctggag
     2161 tgggccccta aatttgcgga agcaggtatc acggtggtag ataattcttc agcctggaga
     2221 atggatccct ccaagcccct ggtggttccg gaaatcaatg cagatatcct gaccgtcaat
     2281 gataaaatta ttgcaaaccc taactgttct actattcaga tggtggtggc tttaaacccg
     2341 ctgcataaaa aatatggcat taagcggatc gtggtgagca cctatcagag tgtaaccggt
     2401 actggtgtga aagccgttga acagttgcat ggtgaaagaa ggaaagcagt aaaaggggaa
     2461 accaccgact acccgatggc ctataaatat ccgattgacc tgaacgtgat tccacagatc
     2521 gatgtattcc tggataacgg gtataccaag gaagaaatga agatggtgaa agaaaccaat
     2581 aagatcatgc gggacgactc catccgggta actgccacta cggtacgtat cccggtaatg
     2641 ggcgggcata gtgaagccgt gaatgtggaa tttgccaatg actatgacct ggctgaagta
     2701 aaagccttac tggcagcttc tccgggtgtt gtggtggtgg atgacctggc caaccagcaa
     2761 tatcccatgc cgatggatgc ccatgaaaaa gatgaggtat ttgtaggtcg actcagaaga
     2821 gatgaaagtc agccgaacac cctcaatatg tgggtggtgt ccgataacct gcgcaaaggc
     2881 gcggccacca atgctgtaca gattgctgaa tatctaaaca gtaagggctt actcggctaa
     2941 tgcgtgattg ataaaatgaa tcagcggttg cagtgcttca aaagctgcaa ccgttttttt
     3001 tacgaggtct ttggaactga ggtccgtgtc tttcatcgga ctgagtgcta caaaactctt
     3061 tagttttaag tagtcgccgg ccgggttatc ggcttcatag ccttttggca atcgtaccaa
     3121 tgtgtacgct gcagaacggg aaagatcccc ataaacagcc ttgaattttt tattcgccag
     3181 tatttttttg aaatcggcat agttataatc gatctcctgc ctgatcttac tcagctgcgg
     3241 cggcattggc atccataaac caccacccat aaaagcctgc ccgggttcgc agtgaaaata
     3301 aaacccggca gtagtggctt tctttccgcc cggattgaaa tacgctccca tattggtttt
     3361 gtaaggtgat ttgtctttag agaaccgcac gtcccggttg atccggaata aacagttttt
     3421 ggccagcata ctgctgtaag ccggattttt ctttccgaat tcgttgatta ccttctgcac
     3481 aaaatccgta taatcagctt ttgcagcttc atacgttttc cggttttttt caaaccattc
     3541 cttattgtta ttcttcttaa gtgccttgag aaaattcagg gttgcaggct ggagcatgag
     3601 ggaataattt ccgttaaagg tgaagcatat aagtcaaaat tcaaaagtaa aaagtaaaaa
     3661 taacaggctg aaaggctgaa tagtatttat gcgttcgagt gtcaggcgta caaggaatgt
     3721 aatccctcat tacgacacga aatatcaaac acagcgtata agtaacagat tcttcttaga
     3781 aagggctgtt gactcccgac tactaactcc taactcccga ctcacttaac tcttccccaa
     3841 ttctccccac tcttaatcct atacaatgtc atttcactca cgccatattt ttcagcaagc
     3901 tgacggatac tcagggtgcg ttttttactg gcaagggtgg ctttgatctt acttacctga
     3961 gaagcggtga gtttaagacc agtggtctta ttggcctgtt ttattttgta cgcttttttc
     4021 gccggactgt tttgctgatg cgccatcatt tcttccaggt tggcccactt caggttcttg
     4081 acatcattgt ccagtttatt gtggttctta tggatgacgt atttgtgagc cgcagattta
     4141 cggtccacca tcagtttcgc gatttcccgg tgaatgtaca gcgtgccttt gttgtcagga
     4201 cgatggaggt tgagtgtgcg gtagccagtg gttaaggaac cgcttaataa ttttccgtcc
     4261 gttatgtctt ctttgtagct ggcaatccgg cccttactag atacggcata acgatttctc
     4321 agttgcctcc atccggagaa actcaaaggt ttccagactt caccggctaa ttttttgatc
     4381 atggtgttaa gtttttttta gccaggaaaa ggtttgttca ctaacggcgg tatatgcagg
     4441 ttaatagtgc aagtcaatca gtactaagtt agaccatatt cgctttaaag acaattatta
     4501 ttttttaccg atcaattgta aaaattcatc ctctgagata atcttgaccg aatttatttt
     4561 ttttgccttt tccagtttac tgcccgcatc ttcccccacc actaagtaat tcagcttagc
     4621 acttacacca cccagtatct gtccgccttg ttcttctgcc atggcttctg cctggcttcg
     4681 tttcagggtg ggcagggtac ctgtaaagag aaaggtttgt ccggtcagac tgcccgtact
     4741 actcatttcc ttcttttcgt ttttcagggt taagccgagt tgctccagtt caagcagcat
     4801 ggtgatattg tcagggttgc tgaaaaactg gaaaatgctg ccaccgactt tcggccctac
     4861 atcctcgagt gcctgcaact gctccagcga aaacccttta aaatccagca aatggtttac
     4921 ggcttgtgcg agcgtctttg ccgtggtttc gcctacaaaa cgtattccaa gagcataaat
     4981 caaccggtga agcggttgtt tttttgaatt tgtgatggct gattgaagat tatcgatcga
     5041 tttctgacca aaaccctcca atcctttcag tttattaaaa tcaagaatat atatcgaagg
     5101 aatatctttt aataaaccca tttcataaaa tttccgcaca ttggcttctc caaaaccccg
     5161 gatatccatg gcatccttgc tgacaaaatg gatgatccgt tccactacct gggccggaca
     5221 ttcaatattg gtacaacgcc agactgcttc ttcttcctct ttgaacaact ggctggcaca
     5281 aacgggacat tgtttaggaa actgaatagg ttgttcctgc ccgttgcgca attcgggcat
     5341 ggacttaacg atctggggga tcacatcacc ggcccgttcg atcagcactg catccccgat
     5401 ccgcaggtct ttttcccgga tatattcttc attatgaacg gagatactgc ttactgttac
     5461 tccacctacg gccaccggtt caagtttggc caccggggtg accgccccgg tgcggcctac
     5521 ctgaaattcc acattcagta atttgctggt ggcctggcgg gctttgaatt taaaggcaat
     5581 tgcccaccgg ggatgatggg aggtcatgcc cattttatcc tgaagggcaa aatcatttac
     5641 cttgataacc atgccatcta tctcataggg caggtcatcg cgggtggctt caaattcctc
     5701 cacataccgg attacggcgt caatgccctt aaatactttc ttttcctgct ccggactgcg
     5761 gaaacccaat tcccaaagca gctttaagga accggcatgt gtggtcaggt ttaccggaag
     5821 agcagcctct ttttgcaggg tgtaataact aacatgatag acaaaggctt caagattacg
     5881 cttggccact tcagcggaat ctttcagtcg taaggtcccg gaagccatgt tccttggatt
     5941 ggcctttggg tcttcgccca gggcaatgag ctggtcattg tattttttaa aattcgattt
     6001 attgatcagt acttctcccc ggatctccac ttgctggatg ccttgtctgg agaatgctgc
     6061 tgacaatgga atggttttga tctgacggat attgttcgtg atctcatcgc cctccacgcc
     6121 atcgccccgg gtggcccctc tgaccagtaa atcattttca aaaatcaggg agatactcgc
     6181 gccgtcaaat ttgggttcca cacaatattc gatttcgtcg agtccggtga gttcccgggc
     6241 tttccggtca aaatcctgca gatcttccgc attgtaggaa ttgtccagcg acaacatggg
     6301 caccaggtgc tgtaccgtgg ggaaatcctt ggtcagtcct ttggctaccc gctgggtggg
     6361 ggagtctgcc gttaccagta ttggctcttc ctgctcaatt ttttcaaggg ctttgtataa
     6421 ctggtcatac tcaaaatccg caatcagggg ttcggtaagc acataatacc ggtattcatg
     6481 aaaacgaagc acttcccgca gatcttccac ccggctggcc ggtatcgacc cggtttttaa
     6541 caggctaatt aattcatttg taagatgttg ggttgctttt gtgtgctcgg ctgaatacat
     6601 agtcaggggt cttgacccgt aaagttaatt ccctgagtaa tataaaaatt tgccgtttat
     6661 acagaattga ctcctaaaat taatgtgtcg ttagtacata tttgttaact tcgaatcggg
     6721 atacccaggt tgcttataat agcccgggaa tctgaattgc taaaaaagct tgctatgaaa
     6781 agaacgatcc ctcttttaat tgcattggta ttttttgtcg tgaaggccaa tgcccagtta
     6841 ctcacctgga ctcccgattt tgccaaggat aatgataata tcgttataac catggatgcc
     6901 agtaagggta accagggctt gctgaattac agcactactt cggacgtata tgttcacatt
     6961 ggtctgatca ccagtgccag caccagttcc tccgactgga aatacgccaa gtttacctgg
     7021 gccaccacac ctgctaccgc acaggccacc tatataggta ataacaaatg gacctatacc
     7081 attaataatc ccaggacttt ttttaattca cctgcgggtg tgccttccgg tgaaacgatt
     7141 cagaaaattg ccattctgtt ccgcaacggt gccggaactg tggtccagcg gaatgccgac
     7201 gcgagtgata tgtttattcc ggtatatgat aataccctgg ctacccgctt taccgtgcct
     7261 ttctttcagc ccaccagtac ccggataccc gaaccgatca ccaagcaact gggtgataat
     7321 atacagctga ccgctattgc gaatatttcc tccaccatga aattgttcct gaatggaacg
     7381 gaaatacaaa ccgcttccgg ggtaactact atttccgcaa acccggtatt gactaccggt
     7441 ggtaaccaga ccatacgtgt ggatgcgatt gacggagcga ccactaaatc agaatccttc
     7501 tccttttttg tttccacccc tgtcaatatt gcgcctttac cggccggtgt gcgggatggg
     7561 atcaattatg aagccggcaa tacctcggcg gtgctggtct tatatgcacc caataaaaca
     7621 cgggtatcag tgatcggaga gttcccggga agtaactggg ccgagcaaag taattatgtg
     7681 atgaacaaaa cgcccgatgg caattactgg tggctgcggg tcagcggact tacagcgggt
     7741 accgaatatg cgtttcaata cctggtggat ggcagcctga aagtagggga tccctatgct
     7801 gaaaaaatac tggatccgaa taatgataac ttcattacat ccaccgttta tccttcactg
     7861 aaagcctatc ccactggttt aacaacgggt attgtaagcg tactgcaaac caatgcaccg
     7921 gcttacgcat ggaccgtgaa taattttgcc cgtccggata aacgtaacct agtgatctac
     7981 gaaatgctgc tccgtgattt cattgaagcc catgactgga aaacacttcg cgacaccctg
     8041 aattattttc agaccatggg ggtgaatgcc atccagctga tgccgatcaa tgaatttgat
     8101 ggtaatgaaa gctggggcta caatccttct tatttcctgg cccccgataa atattatgga
     8161 ccaaaaaacc ggctgaaaga atttattgac agttgtcacc gccgcggcat tgccgtgatc
     8221 atggatatcg ccctcaatca taccaccggt gataatccgc tggccaaatt gtattggaac
     8281 agcagtacga atcagccggc ggcgaataat ccctggctga atgtatccgc tacgcaccca
     8341 tacaatgtgt tcaatgattt caatcacgaa agcctggcca cccgctactt taccagtcgc
     8401 gtggtggaac actggctgca ggaatataaa gtggatggct tccgcttcga tctctccaaa
     8461 gggtttaccc agaatgccca atgtggtagc agtaccacga atgaatcctg tatttcgcaa
     8521 tatcatgcag accgggtggc gatctggaag cgctattatg atacctgcca gttaaaagca
     8581 gcgggcagtt atatgatcct ggaacatttt gccgcggata atgaagaaat tgaactatcg
     8641 gattacggga tgatgttatg ggggaatgac acgtataatt ttaatgaagc ttctatgggt
     8701 tgggtcaata cgtccaactt tgaacgctat ctctataatg cccgcggttg gagtaagccc
     8761 cacctggtaa gttatatgga aagccatgat gaagaacggc tgatgtacaa aaacctgaat
     8821 ttcgggaata acagcaacag cgctcataat gtacgtgacc tggccacggc cctgcgccgg
     8881 attgaacttt gtgccggatt cttcctggca gccccgggtc cgaaaatggt atggcagttt
     8941 ggcgaacagg gctatgattt ttccatcaat cgttgtacag acgggacgat caatacgaat
     9001 tgccggttga gtaataaacc catccgctgg gattatttaa ccgtaaccgg acggaaaagg
     9061 ctctatgata tatttagcag tatgaacaaa ctccgggcac atccctggta caaagatgtg
     9121 tttacggcca ataatataac catcaaccgc agcctggcgg gtgcatttaa gacactcacc
     9181 ctgcgcagtg ccacggatac ttctatgtta tgtgtgattg gtaattttga tgtgaatgca
     9241 caaacgggtt cgtttacctt tcccaatgcc ggcacctggt atgattatct cgggggtggt
     9301 acattcacgt ccaccggtgc tgcacagagt tttaccctgc aacccggcga attgcatgta
     9361 tttgtaaacc gcaacctgac taatgcagtg gttaccgccg ataccagtgt aacaccggca
     9421 ggtaatcggt ttgcagctca gctttacccc aatccgggag tggctgcatc acaactggaa
     9481 attgaaatgc cccagaccgg cctggtacgg gcagaaatag cggatatcag cggccgtaaa
     9541 atcggtactc ttttatcaca atccctggca aaagggaagc accggatagc tattggagaa
     9601 aaaataagta tgttgccggc cggaatttat attgtgtcca tccggaccgg aacaaaaacc
     9661 atccagttaa aactggcgat tcaataacag accaaatgct gcagcgatga cggagaaagc
     9721 tttacaacaa ctgaagaccg cggaaacgga aaagatcagt caccttgatt atttcaatat
     9781 tggtatatcg attgactgtg tgatactcgg ttatgaagag aaagagctga aagtcctgtt
     9841 gatcaaatcg gatatgcagg aattttccgg actctattcc ctcctgggtg attttataaa
     9901 acccgacgag gacctggaag cggcttctta tcgggtgtta aaggaaagaa ccggactgga
     9961 tgatgtgttc ctggagcagg taaagacttt cgggaatatt tatcgtcacc catccggtcg
    10021 tgttattacc gtggcttatt attcactgat agatgtcagc aatcataagc tgcagctgga
    10081 cctgaatgaa ctgcactggc acaaagtgga tgaaataaat caactggcct tcgaccataa
    10141 actgatcctg gacacctgtc tcggccgttt acgcgaacag gtgatggagc atccggttat
    10201 ctttaacctg ttgccggaaa aattctccct gcgtgaatta caggaacttt atgaagctat
    10261 actgggagtg gaactggacc gcaggaactt ccggaaaaag atctccatta aagactggtt
    10321 ggtggatatt gatgaaatgg aaaatgatgt accacaccgt ccgggtaaac tgtatatgct
    10381 gaaaaagcag tatcgcaaca aagccattaa agcaactatg cctttacaac tggcctgact
    10441 taaagcgagc cgccggttac cagccgcgat ggaagaataa ttgaacgtga agggctgggt
    10501 ccttccatga tttccagcat acgcttcacc gataatttgc ccaattcaaa cccgcttgtt
    10561 tcgatataag tgatttccgg tttataaaga ccagggataa agccattgct gatggccagt
    10621 acggagagat catcgggaat atgcaggtcg gtctgataaa tactcttcat ggctccgatc
    10681 agcaattcat cgctcataca aaaaatatga tccggcctgt ttttcttacc tagtacttcc
    10741 gttgtgattt tgcgggcttc cgcactgttc aggcaatgac gaatatccag ggagggtgct
    10801 gatttttcct tttcgaagac tttcagaaag gcgtcctttc ttttcctggt gatcgagaga
    10861 tctgcattgc ccaacagcgc cagtactttt ttctttttat acttcagaat agtatttgcg
    10921 gccatggtag cggcttcttc atccgccata cagatagtgt caaagccggc atcatcgggt
    10981 actttatcaa agaataccac cggggtacct gactctttta atttattgta taaaggggcg
    11041 gagttgctgt cggccaccag cgagacaaac acaccatcca cccggttcag cttacaaagc
    11101 ttcaggtttt ccgattcctg caggggatca tcaccggatt gcaggatgat gagtgagtaa
    11161 ccgcaacggc gggcttcctc ttccacggct gcaataaagg aatcataaaa caagttggaa
    11221 ataacaggaa cgatcaaccc gaatacccgg cttttattgg tacgcaggtt cacggcatac
    11281 gaattgggct catattccat gagcaaagcg agttccttca ctttccgttt agtgttttca
    11341 gaaatatcgg gatgattctt caacgctctt gacaccgtag aaatactgat gttcaattgg
    11401 gttgaaattt ttttaagtgt ggcgggactt cccataagca ggcaatcgta tgaaatataa
    11461 aagtaatttt tccaccgcaa acatttgcgg aaatgttttc ggtctttttt cgcgacccaa
    11521 aacacgttga atgtgttctt gatacatcta aataactata aatttatccc ggaatgagaa
    11581 ttttaacatt ctttttttca acgcagcaat gcaaggcgaa aaattgactg aatgaaagaa
    11641 tgatagaatt gccaggccag tcaggttgaa acaaaagccg gtcccccctt atagttccag
    11701 tgttacagac ggatcaggcc atatgaaatt tggattatta caccgattat aatttttaaa
    11761 aaaatcgatt gcttatgtct gcaaaaacgc tgctttctgc tatcctttgc cttgtatttg
    11821 cggtctccgt gcaggttgta tctgcccagg acagaaccgt aacaggtaaa gtgactgatt
    11881 caaaagatgg ttccccggtg tccggggctt ctgttcagcc caagggctcc aaaacaggaa
    11941 cgtctacaaa acctgatgga accttttccc ttaatgtagg tgctggtgtt tccgaactgg
    12001 tcattacatc aattggttat gaaacacaaa cggtttccgt ttccggaaag aattccgttg
    12061 acgtgtcatt tgtagccagt gcaggttcca gcctgaatga ggtagtggtt accggttatg
    12121 gtacaaccag gaaaaaggac ctcaccggtt ctgtttcttc tgttaaagcc aaagacttta
    12181 acaaaggaac agttacatca cctgatcagc tgattcaggg taaggtagcc ggcttacaaa
    12241 ttgtaaataa cagcggccag cccggaggtg gtgctacgat ccgtattcgt ggtaccagtt
    12301 cagttcgttc cgggaataat ccgctgattg ttgtggatgg tgtgccactg gatggtggta
    12361 cggcccgtcc cggactgggt gttggaggtg ttggtacaag tcccgcatcc aacccgctta
    12421 actttattaa tccaagtgag atcgccagca tggatgtgct gaaagatgcg tctgccacgg
    12481 ctatttatgg ttcacgtggt gcaaacgggg taatcatcat caccaccaag cggggtcagt
    12541 caggtgctcc ccagattgat gtaaattata cagccggtac ttcttccatt atgaagaaac
    12601 tggatgtact taccggggat gaatacaggg ctgcattgac tcaatacgga cttacttccg
    12661 ggaattatgg cggaagcgtg aacgcaatgg atgagatcat gcgcaaaggc atgacacaga
    12721 acatttccgt tgcagccagt ggtggtaatg aaaatgccaa ataccgcttt gcggccagtg
    12781 ccctgaatga ggaaggtatc gttaaagagt caggacttaa aaaatataca attgatttca
    12841 aaggaaactt caaactgctc gagagcaaaa aactcggtct ggatgtttcg ctgatgactt
    12901 cacaggtgaa agaatcagtg gtgcctatca caaccgatgt gggttttgaa ggtaacctga
    12961 ttggtcaggc attgcaatgg aatccgacgc atccgatgat gaagacagat ggtacattct
    13021 ggtctgaacc ccagtttggt gccacatcta tcaacccgct ctggttcctc aaaatgtatg
    13081 atgaccaggt gaatacgtca accgtattgg ccagtatatc tccctattat aagttcagca
    13141 attcactgga ataccggttt ctttacagct tcaatgaaag taacggttcc cgtaaagcgc
    13201 agattggccg tcagttaacc aatatccctg cgtttaaaga caggggatgg gcatctgtaa
    13261 gtgcgggtaa actcaggacg cagcagttca cccatacatt aaacttcaac aaacagctca
    13321 gcaatgcact gaatctttct gctgtagccg gttatgagta taccaagttt gagtttgcca
    13381 acaatggcca gactgcacag gatttcatcg acattggtat tccctattat aatatgctgg
    13441 gctatgccac acaggccagc cgggcgatct ggaattttga aaatcccaca aacgaactgc
    13501 aatcttattt tgcaagaaca tcgtttaacc tccgcgacaa gtacctgctt accgccactt
    13561 tacgtgcgga tggttctact aaattcggag agaataataa atatggttat ttcccctcac
    13621 tggccgctgc ctggaatatc agcagtgagt cctttatgca gggggtggat tttgtcagta
    13681 acctgaaact ccgcttaggc tggggccgta ccggtaacca ggaattccct gccggttcat
    13741 ctcagacccg tttcggtgta acaggaatcg gttccatttc ccagttaaac gtgggtaatc
    13801 ctgatctgaa atgggaaaca tcgtccacta cgaatatcgg ccttgacttc gcattgctga
    13861 atcgtaaggt gaatgtttca gtggattatt tcaacaagct tacagaagat gtgctgtttg
    13921 aactgagttt cccggtaccg ggtggttcat ctgcacgtat ctggagaaat cttcctgcaa
    13981 ctatcaagaa taacggtata gagatcgcgg tgaatacgaa cctcgtcagc aaaactgatt
    14041 tcagctggaa cctgggtgta aatgccactt tcctgaagaa tatgctggaa ggattaaatg
    14101 gtacctacaa tacaggcgca ctccatggcc agggaagtac cggtgcattt gtacagaaac
    14161 tggcttccgg ttatcccctg aatgtgtact acctgcgtga attcatgggt atcgataaga
    14221 caaccggtca gagtatctac caggaagacg gtgacgtttc ctactatggt aaagatgcca
    14281 acccgagaac actgctgggt cttagttctg aaatgagcta taagaaattc ggactcgtga
    14341 tcaatatgaa cggagccatg gggcactacc tgtataataa tacagcacag tccgtagttc
    14401 cgatcaccaa cctgggtagc cgtaacgtgg ccagctcact gatcagtgct tctgtaaagg
    14461 aagacctgtc aaacccgatc acttcttcaa cccgtttcct ggaaaaagga aattacctga
    14521 agatggccaa catgacgctg tcatacgatc tgggccagtt tgccaaattt gtgaaaggag
    14581 ccagggtgtc gctgaccggt cagaacctgt ttgtgatcac caacttcacc ggcttcgatc
    14641 cggaggtaaa cgtagacaaa aatatcggtg gtattccttc aaacggtatc gagtatattc
    14701 cttacccgac tgccagaagg atccagctga atttcaattt ttcactctaa ttgctaaaac
    14761 ttaaaaacat taatatgaaa tacggtaaag taatattggt tgcttcagct gttctttcat
    14821 taacggcttg taccaagctg gatgaaaagc tgacaagtga gacgacttcc ggtaataccg
    14881 gaggctccgg aagcgtgaac gtatcggccc tgcttgtagg aacctatcgt tcatttgaag
    14941 gattgtatat ggatcaggcc cgtatctggg ccgcccagga acattcatcc gatgaagcag
    15001 taggcccgac acgtggcggg gactgggatg ataacggtat ctggagggtt tatcataatc
    15061 acaaatggac tgctgatcat gatttcctca ctgccacttt ccgtcagctg gctcagtcac
    15121 agtttgcggc cacaacggta ctcgataata acccgtctgc acagcaggct gccgaagcaa
    15181 aattcctgcg cgcttttgcc atgtggccgg tattggattg ctgggaccag gtgcctttcc
    15241 gtcctacatc aaccggtgat ctgacaacca cacttcctga agtaaaaaaa ggaaccgaag
    15301 ccgctgattt tattatttca gaactgaatg ccgcgattcc gaatcttccg gccggaagag
    15361 gcctgggtat cgccagtaaa gatgctgccc gtgcgctgtt aatgaaggtt tacctcaaca
    15421 aaggcgtata tgcaaacagg gaaaatccgg tatttgcacc tgctgatatg aaccaggtga
    15481 ttgcgctggc cgaccagatt ctggcaggca gctattcact gggcaacaac ttctttgaca
    15541 actttgcccc gaataatgac gtaatcggtt cagaactgat ttacaccctt aaggcagata
    15601 acggagccgg tgtgggaggt aatggtgcca tgtattccta tatgtgcggt ctgcattaca
    15661 ataccaaccc cagtggctgg aacggttttg ccacgctcgg tgatttctat gataaattcg
    15721 aggccaccga taaaagaaga ggtggtactt atgcaggtct taccgatgtg agcggattaa
    15781 aatcaggttt cctgatcggt cagcagttta atcagaatgg tacggcatta caggaccgta
    15841 aagggaatcc cctggccttt acccgcgaag taaaactgaa agaaaccgga aataacctgg
    15901 aagtaaccgg tatccgcgtg atcaaatata tacctgacta tgctcaccag ttcccggccg
    15961 ataatgacat ggtgatcttc cgccttgcag atgtactgtt aatgaaagca gaagcgatcc
    16021 tgcgtggtgg tacagctacc aacgtaggtc cttaaggagg tacagctgcc gcgattgtaa
    16081 actatatccg gacacatacc acccgcggtg tggctgcctt aggcaccgta gacctgaatg
    16141 cggtttatga tgaaagagga cgtgaacttt actgggaagg atggagaaga aatgatatga
    16201 tccgtttcgg aaaattcctg gctccgagag aactgaagcc tgaaaccagc ggtgtggaac
    16261 gtttattata ccccattccg aacgaacagc ttaccatcaa cccgaacctg acacaaaacc
    16321 ccgggtatta acaattttca gctaagcagt tagcaatttt agcaattaca aaagccattt
    16381 tctaatggct tttttttatg gggtaattgc agggttcagc cgttttacca gcataaaatc
    16441 tttagatttg acctgtgtgc ttaatcagcc gattatcgta ttatgagaaa attgatttgt
    16501 ggtctttctg tctgccttac actattagcc ggatgcgggg attcaggcga tgagaaaaat
    16561 cttttttcat tgcagcagaa tacgggtatc cgttttcgca acgaggtcag caactcacct
    16621 gatttcaata ttttcagtta ccgtaacttt tataacggtg gcggtgtggc tatcggggat
    16681 atcaataatg acgggctcgc cgatgttttt tttaccgcca atatgggcag taataaactg
    16741 tttctgaata aaggggactg gcaatttgat gatatttcag caaaagcagg ctttgttaat
    16801 aagaaagact ggagcaccgg cgtggtgatg gtagatatca atcaggatgg ctggctcgat
    16861 atttttgtat gtaatgccgg ttatatcaac ggtgcgaaac cccgttgcca gcttttcatc
    16921 aataataaga atctcagttt tacagataaa gcggaagaat acggactgac caatgaaggt
    16981 ggctatacca cccatgccgc tttttttgat tatgatgcgg acggggatct ggattgcttt
    17041 attatcaata acagctttat cccggtcaat acactgaatt atgtcaataa gcgaaacctg
    17101 cgggctgctg actggcccgt tgctgatttt ctgaaaggcg ggggcgatca cctgctgcgg
    17161 aatgatgcgg gaaaatttac tgatgtaagt aaggaagcag gcatctacgg cagcctggtc
    17221 agctttggtc ttggtgttac cgtgggcgac ctgaatggag atcactaccc ggatgtatat
    17281 gtttccaatg atttttttga gcgggattat ctgtatatca atcagcgtga cggaaccttt
    17341 aaagatgaac tggagcaatg ggtgcagcat gtgagtcatt cttccatggg agccgatatg
    17401 gcggatgtga ataatgacgg ctatcccgac atctttacca ccgatatgct cccggatgat
    17461 gattaccggc tgaagaccac caccacattt gataatatcg atgttcaccg gctgaagcaa
    17521 aaatccggtt tttacaacca gtatactcag aataccctgc agataaacaa ccgcaacggg
    17581 aagtttatgg aaaccggttt ttatagcggc gttgccgcat cagactggag ctggggcgga
    17641 ctgatatttg atgcggataa tgacgggcgc tccgatattt ttgtttgtaa cggcatctac
    17701 catgatgtga ccgaccagga ttttattgat ttttttgcca atgatattat ccagcgcatg
    17761 gtgatgaccg gcgagaagga taagtttgat gagatcgtga ataaaatgcc ttcccgcccg
    17821 attcctaata aactctttct taaccagggg gatctccgtt ttgccgaccc gggcaataag
    17881 tcaggcctcg gtaccccttc cttctccaac ggatcggcct atggcgatct ggataacgat
    17941 ggtgacctag acctggtggt aaacaatatg aatatggatg cctttgtata ccggaacaac
    18001 agccgggaaa ttaatcagca tcattatatt ggcatcctgc tgaaagatac gggccgcaat
    18061 acctttgcgg tgggaagtaa aattcaggta taccagggaa cacaggtgtt cagccgggag
    18121 gtcattccat caagggggtt ccagtcatcg gtcgattacc ggcagatcat cgggctcggt
    18181 aacggtgtcg cggattccat gctgatcacc tggcccgatg gctcatcctc ccgtatcatc
    18241 cttcctgaaa taaataaagt acatgtgata acggccgccg ccactgcaca acacacgttg
    18301 actgcatcgg tcgccagcca gcccttcttt gcgttaacgg actttccggt gtttgataaa
    18361 cataccgagg acgatttcat tgacttttat aacgaacgga atattccggt tatgctttcc
    18421 cgtgaagggc cgcatgcgga tactgcggat gtgaacgggg atggattatg cgacctgtat
    18481 atcggaggcg cagcgggtca gcccggacaa ctctatctgc agacggtaac aggttcattt
    18541 ttgaaaacag tatctcctgc ctttgaagcc gataaagcct ttgaagaggt ggccgtatta
    18601 ttttttgatg ccgataacga tggtgacaaa gacctcttca taggggccgg gggtaacgct
    18661 cagcaacccg acagccgctt actggcccat cgcctgtacc tgaatgacgg aaagggaaac
    18721 tttgcaaata gcccggttaa acttccggcc aatactatga acattgcggt ggccgttgcc
    18781 catgatgtcg atcgagacgg tgatcaggac ctctttatcg gggcccgtag tgtgccgatg
    18841 caatatggta ttgtgcctga cagttatttg tggatcaata acgggtcagg tgctttcacc
    18901 gccggctccc ctttgaaagc aggaatgatt accgatgcgg tttttgcaga cctgactggt
    18961 gatcagcagg ctgaactgct ccttgtgggt gaatggatgt caccccggtt gtatacgatc
    19021 caaaatcaac aactgactga agtgaaaacg gatctctcct ccctgaaagg ctggtggcaa
    19081 tcggttgcgg tggcggacct ggatggcgat aagcgaatgg atatagtcct gggaaatacc
    19141 ggagaaaatt tttacctgaa acccactccg gagcaaccgg tacgactctg gatcagcgat
    19201 tttgatgaga atggcacagc cgataaaatt attacccgta ccgtaaataa gaaagatatg
    19261 ccggtctttc taaaaagaga ccttacagat cagctcccgg tgctgaaaaa acaaaacctg
    19321 aagttcgaag cgtatggaaa taaaacaata caacagctct tccccggcga agtcatgcaa
    19381 aaagcacaga tgtatgaatt taacagtggc gcttcttgta tggcctacaa cgagggggat
    19441 gggcggtttc tcattcagca gttgccggtt ccggttcagt tttcttcggt aaacgcaatt
    19501 gtctgcaatg actttaatca ggatggacgt cctgacctgg tactgggagg aaatcagttt
    19561 cactttcagc cgcagttttc aagactggat gcgagttacg gacatttctt aattaataaa
    19621 ggccgcagtc agtgggagtg ggtggccccc cggcaatccg gacttgaatt gcgcggtatg
    19681 gtacgggata tccggctcct gccaataact ggccggactg gtctcctttt tttacagaat
    19741 gatgagtcgc ctgtttttta tacattcagc ggggccatta aataacgatc aagcatgttc
    19801 agacaacctg cgcatattct tatagtatgg tgtacactgg tcacaggact gttctcctgc
    19861 aaggatacag agccggctgg cccggttttg tttgaagcac tggatcacac ccgcaccggt
    19921 ctgcaggtaa gcaatcaatt gaccgcaaga cccgacttca atatgttgaa gtatatgtat
    19981 ttctataatg gagcgggagt gggcaccgga gatttcaata aagatggact gaccgatctg
    20041 ttctttgctt caaaccaatc gcagaacaga ctttacctga accagggaaa actgaagttc
    20101 acggatgtaa ctactcaggc cggtataccc gatgacggag gctggacaac cggcatatca
    20161 gtggccgatg tgaatgatga cggctggctg gacatctatg tatcaagggt gggacaatat
    20221 gaaacactga aaagtaaaaa tcaactgctg atctgtaagg gattggataa agaagggata
    20281 ccgctttatc aggatgaagc agcggcttac ggacttgatt tttccggctt cagtacccag
    20341 gctgcctttt ttgactatga cctggatggc gatctggata tgtttctgtt aaatcattcc
    20401 ctccggtata acagcacatt tgcaccacgt agcaatttta caggcaccgt ggattcgttg
    20461 tcccgcgata ttttatacag gaatgataac ggaaaatttt ccgatgtttc caccgccgcc
    20521 ggcattcacc agtcggtgat tggttatgga ttgggaattg ttatcagcga cctgaatctg
    20581 gatggctggc cggatattta tatcggcaat gattttcatg aaaacgatta cctgtatatc
    20641 aacgatcagc aaggaggatt ccgggaagaa ctgaccgaac ggaccatgca taccagccaa
    20701 ttctccatgg gagtggatgc cggggatatc aacaatgatg gctggccgga tattattgca
    20761 gcggatatgc tgccttctga tccctatatt ctccgccgct cgctgggtga ggatgaatac
    20821 gccacttttc agatcaagat caggaatcaa tataatcatc agtatgcccg gaataacctg
    20881 caactgagcc gacggaacgg attatacagc gaaatcggat tgtacgccgg tgtatccgct
    20941 acggactggt catgggccac cctgtggatg gattttgata acgatggccg gaaagacctg
    21001 tttgtctcaa acggcattcc aaaacggctg aatgatattg attatgtcaa ccatgtttcc
    21061 aatgatgaaa ttcaggcaaa gatcaggtcc ggcaatatgc aggacaagga tatggccctc
    21121 attgataaat ttccagagat ccgtttgaag aaccggtttt ttctgaacaa ggggcaggcg
    21181 tcttttgaag atgcaggaaa caggatcggc agtaatccgg agaccttttc taacggggcg
    21241 gtatatgcgg acttagacaa cgacggggac ctggatatcg tggtgaataa tattgatgaa
    21301 cccgccgtcg tatacagaaa taccagtaat gacaacgggg aacatccctc gctgcaatta
    21361 caactcaacg gaccggcggg aaaccggcag gcggttggtg ccaggctgat tgcttattcc
    21421 ggaaaccagg tttttaattt tgagaaattc caggcccgcg gatttcaatc atccatggag
    21481 acgcccctgt ttgtcgcact cggcaatcaa aaaccggatg ctgcctggct gatctggccg
    21541 gataatacct gtgagcctgt ttcgcttaat attgacacgg cacagataaa gacgctaacc
    21601 taccagccgg ggcttccccg ttttgattat gcgcggctgc gttcctttta tgcagcaaca
    21661 gaagtgccat ttgaggatat tacccggcaa accggactga cgtttatgca ccgggagaat
    21721 gtatttaatg aatttgaccg ggagcccctc atgccgttta tggtttcccg tgaaggacca
    21781 gccatcgccg tggcggatat aaaccaggat ggactcgaag acctgtttgt gggttcggtg
    21841 aaatgggaac agggagcggt ctttatacaa aataagaacg ctacgtttac acgcgtgcca
    21901 caaccggccc tggccgccga tagcactttt gaagatactg atgcctgctg gaccgatgta
    21961 aacaaagatg gatacccgga tctggtcgtt gccagtggcg gtaatgaata ctacgggcag
    22021 acaccctatc tgctttcacg tgtatacctg aatgacggga aaggtcatct ttccaaaaaa
    22081 gaagatgcat ggccgaagga actgatgatt tccgcatccg ctgtgctgcc gctggatttt
    22141 aatcaggatg ggtatatgga tttgtttatc gggggcagat cggttccctg gcaatatggt
    22201 gccattcccg cttctgcctt actacagaat gatggtaagg gcaggttctt caatgttacg
    22261 gaaaagatag ccccgcaatt ggcaactgca ggattggtga aagatgcagt gattgccgat
    22321 gtaaataacg atggccgtac tgacctggta ctggcactgg aatgggaggg gatcgttgct
    22381 ttcctttata aagaaggtca atttacccgg caaaatatcg tggcagataa agggtggtgg
    22441 aattttatct tgccggctga tattgataat gacggggata tcgattttat cgcgggtaac
    22501 cagggactca atagccggtt tacagtttcc aaaacgcagc ctgtacggct ttactacaat
    22561 gacttcgatg acaatggacg aaaagaccag gtactgacct attacctgca gggacaggaa
    22621 ataccatttg ccaataaagc ggaactcgaa aggcaaatgc cggggttgaa aaagaaatac
    22681 ctgtatgcgg aagaatttgc gaaagccggg ataaaagata tgtttagcaa acaaaagctt
    22741 gaccaggcaa ccctgttaac cgccgattat ttttcaaatg ccttactgat caatgaaggc
    22801 aagggcgtat ttaaaacaaa ggccttaccc tgggaagccc aactgaccag ttttcgcggg
    22861 gcctgtatgg ccgatttaaa cggggataac cggctggatg taatactggg tggtaatttt
    22921 tacccgaata atatccagat ggggcgctat gatgccgatt atggttcggt actgatgaat
    22981 aagggacacg gggatttcca ggtgcagcag tcaacactac ccgtaaaagg agaagtgcgg
    23041 caggtggcac ccatccgttt gtcaggaaac ggaacggcct atatcctggc caggaatgat
    23101 gactcactga tggtaatcag gtcagcatcc aatcgttaaa ataccggtga gaatattttc
    23161 agttgtacaa tctgttcatc gctgaaatca tagccgtcac tcaggatttt ctgaaacgga
    23221 atagccatgg aggtgaaatt atgatcagac ttaaaccggt caagggcccg gtaggcttta
    23281 tccacttcat tcagggttcc ctggtagttg gcaacgagca ggtttttccc caggggcatt
    23341 ttcttatatt caataccagt cgccggatcc ggatccatcg cattagtgac cccgatactt
    23401 gcaaataatt tgatatcctt ttcccccttg agtgaataaa aaatacggac cccgttataa
    23461 cccgcatctt tcgaggcagc ataagcaatc agttcatcaa acagtttccg ggtggcttcc
    23521 cgcttcgtgg ccaaaggcac agaaacggat ttaaaaagaa aagcggtgtc ttccactttc
    23581 accagctgga tctcataccc gtaaaagcgt ttgctgtctt ccatataggc tttcagattt
    23641 tgcaggcttt tccgggcatc ccggataagc ggattctttc caaaccaatg accaaagtat
    23701 gtattctcat aactaagggt gacattagaa agctgtgcat tcagcgtgtc gccatttata
    23761 gaaaaacgaa aagccttttc ctttccctgg aatcgggcgg tgatccgggc actgaagggg
    23821 gtctgttcac ttacggacaa gctgtctgat tcggcggaag aggaaagata ccaacgttgc
    23881 acctggtctt ttgaccggag ctgctcaccg gttttgagta tagcatagtt tatctcaacc
    23941 tgctcccgga atgtttgttt gctgaaaccg aaaaaagcgg tggcaagcag tagtacagct
    24001 aacaccgtca gcagaaaaag tcttgatttc ctgttcttca tatagcattt atttcgcccg
    24061 ctaaaaataa cctctttcag gcgttatgtt tattttttgt gcgccggatt atcctgttta
    24121 atttcccaac aacaaacgtt tgcaacagcc tgtctgttcc tgtttcagta cggtactccc
    24181 tgctgttttt tgtactttcc gggtccaaac gattgcatat gacattggct aaaccacggt
    24241 taagtttctg gcagatcatt aacatgaatg tgggtttttt cggcattcag tacagttttg
    24301 gtttgcagca aagtgccgtg aatcccattt atgatttcct gggagccaaa acggatgaga
    24361 ttccctggct caacctggcc ggaccattaa ccggactggt cattcaaccg atcatcggag
    24421 ccctgagtga taaaacctgg cacccgcgtt ttggccgcag aacgccctac ttttttatcg
    24481 gtgccattat ctgcagtatc gccctcttcc tatttccctt cagcagtgct ttatggatgg
    24541 cggctggttt gttatggata ctcgacgcag gtaacaatac ggccatggaa ccttaccggg
    24601 cttttattgc cgataaactc gatgccaccc aacagcccac cggcttccag gcgcagagtt
    24661 tttttacagg gttcggacaa acattggcga atttatcgtt gtatgcattt cccttcatta
    24721 tagtcggaac gacaggtaaa ctgcccacct gggtatttgc ctcctttttc ctgggtgcag
    24781 tttgttccat tgtttcgatc tggtggagtg tgcggaccac acccgagatc gtacccgatg
    24841 aagaagagct ggcacaaata aaggaaaaga ataaaggaat gccccatccg gcgattcagt
    24901 tctttctgtc tatcctgacg acgctcgtcg cttatgcggt gatcgtttta ttactcatac
    24961 cttccttgtt catacccgga ttgatccgtc gtttattgaa ttatgtcgaa ggccatgcta
    25021 ctttgaagat cctttttgcc cagaatattg aaatcatcga tgccatcatt aaaatgccga
    25081 aagtaatgtg gcaactggcg ttggtttatt tgttccagtg gtatgccctg ttttgttact
    25141 ggcagaactc ttccaagagt attgccctct cggtatggaa cactacaccg gaagaaaaca
    25201 gtaaactgta tgagaaggcc gttggttgga ccggactggt gaatggctgg tataatatcg
    25261 tcacctttct ttgcgcattc ggactggtct ggtttgcccg tcgttactca cccaagctgg
    25321 tgcattttgg ctgtttgata ctggccggaa tcggattgct ggcattccct tttattgaaa
    25381 acaaatacct gctgtttccc gccattaccg gattcgggat cggctgggcc agtatgatgg
    25441 ggattccata cctgatcgtg gttagtaaga ttcccaaaga acgctacggc gtatatatgg
    25501 gcgtgatcaa tatgatgatc gtaatcccga tgtttatcca gaccttatcc tttggatata
    25561 ttttaaaaaa tttcctgcat aacaatgctg ggtacgcgat tgcatttgcc ggtgtgttac
    25621 tcctgattgc cgcaatactg accttgcgga tacaggccga tgataaaccg gtaaacggcg
    25681 aagtaaattt ctctgcgggt cattaaaaat gaggtatgaa aaaaagccaa ttcaattttg
    25741 ttttcttttt tctattcatt gcagctgctt cctgcaagct catgcccaaa gaaaaggaag
    25801 agcccaaaaa agacggagta aaaaagacat ggtggaagga agccaccgta taccagatct
    25861 atccccgcag tttccaggac agtgatgggg atggggtagg cgatctcaaa ggaattattt
    25921 caagactgga ctatatcaaa agcctcggta ttgacgcggt atggctgaat ccgatctatg
    25981 cctctcccaa taaggacaat ggctatgata tcagtgacta tgaaaatatt cagccgcagt
    26041 atggcagcat gactgatttt gataccttat taaagggctt tcatgaccgc ggtatcaaag
    26101 tgatgatgga tatggtgttg aaccattgca gtgatgaaca taaatggttc atggaagcga
    26161 agcaatcgag aaacagtccc tactataatt atttccattg gtggccggca gaaaaagggg
    26221 agccgcctta ccgtttttcc atttttgatg aaaaaggata tggatgggaa ttcaataaag
    26281 ccacgaactc ctactatctc cattatttcg gcgattttca gccggacctg aactgggaga
    26341 atccgcaatt gcggcaggat atttataaaa tgatgcgttt ctggtgcgat aaaggcgtgg
    26401 atggtttccg gatggatgcc ctggctttta tttcaaaaga caccacctgg cccgcgcaat
    26461 taccagctga atacaagggc agctggccca tgtattatgc gagtggtccg cacctgcacg
    26521 actatatcca ggaaatgaat caggaagtat tgagcaaata caacctggcg actgtggccg
    26581 aagccatggg cgatattccc cgggtgaaga aattcgtgga tgaagaccgg aaggaactga
    26641 atatggccta tcattttgaa gcgatagatt ttggctattt acccggcgaa tacaaaatgc
    26701 ccgatcccaa tggttatgat tttgccagat ggaaacaggt ctatgccaaa tgggacagcg
    26761 cctttaccga caaaggctgg ggcacgatgt acctggctaa tcatgatcag ccacgtatgg
    26821 tttcccgctg gggtaatgac aacccggagt atcgtgatta ctcctccaaa ttactgacca
    26881 cgtttattct cagtatgcgg gccacggcct tctattattt cggggatgag atcgggatga
    26941 gtaatattaa atttgaccgg gtggatcaat acaatgatgt ggaactgctc acgaactacg
    27001 aacaggtcaa agcaaagggc ggtgatctca aacgttttct cgaaggcatg aagatctcca
    27061 gccgtgacaa tggccgcacc ccgatgcaat gggataccac cgtaaatgcg ggttttggcg
    27121 cgggcatgcc ctggctgccg gttaacggta attacaaaac agtgaatgtg tcggcccagg
    27181 agaaagaccc gaacagttgc ctgaattatt tcagggctat gcaaaaactc cggaaggaaa
    27241 atgaagtttt ggtgtatggg aaatttacac tgcttgataa agacaatccg gcggtattcg
    27301 cctatacccg cgaatggaaa gggaagaaat ggctggtatt gctgaacttt aaggataaaa
    27361 caactacggt gaataccggg tttgagttgt ccaaagcaac cgtaaaaatg cataattata
    27421 caacagcatc caccaatggt acactacaac cctatgaaac ggtgatctat gaactgtaaa
    27481 acggataagc atgaataaga aaattgttac ctggtcaatc gcggtaggat taggcggact
    27541 gttgtttggt atgga
//
LOCUS       DAOPMW010000009        27523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1526_length_27523_cov_9.500218, whole genome shotgun sequence.
ACCESSION   DAOPMW010000009 DAOPMW010000000
VERSION     DAOPMW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 27523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27523
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_27523_cov_9.500218"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLU11_01020"
     CDS             <1..533
                     /locus_tag="PLU11_01020"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HPN57714.1"
                     /translation="SQYVSIGADHYTKAVNSNGIKWTVLPDLGRTGSAITTYPVTAPP
                     QQPGGNAPHTEYEFYTYSSGDFRISAFFSPTLNFHNTASGLQYAISVDGEAPRIISIN
                     NDERTTEGGINYKWVGDNIIIKTSSHQIKQAGKHTLKYWLVNSGLVLQKLVLDFGGLL
                     PSYLGPEETINKIVNK"
     gene            536..1684
                     /locus_tag="PLU11_01025"
     CDS             536..1684
                     /locus_tag="PLU11_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPN57715.1"
                     /translation="MKPITLVLLIVVSGIITTGCASGKKTTNVSDSITLKDAFKNDFV
                     IGAAVNTGQINERDSGSAALLRTHFNGLTPENIMKCEIIHPEWDRYDFTLADKLVELG
                     KKYNSPVYGHTLIWHSQLSPFIQKIQRVDSFRTFFTNHITTIASRYDGKLKSWDVVNE
                     ALNEDGTLRKSVFLNKLGEDYIVEAFRLAQKAAPNTELYYNDYNIEQPKKRAGAIAII
                     KKIQAAGVRIDGVGIQGHWRSYNVPMKEIEESIKEFAALGIKVHFTELDLGVLPNPWD
                     NDAADVNMKAEYSAKMNPYAAGLPDSVSAKQAQSYADLFRLFLKYRKDIARITFWGVN
                     DGQSWLNGWPIRGRTNYPLLFDRNNKPKDAFYKVIETKANPVKDKKSF"
     gene            1704..3143
                     /locus_tag="PLU11_01030"
     CDS             1704..3143
                     /locus_tag="PLU11_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN57716.1"
                     /translation="MSTTSQKLSVREKIGYSLGDLAANLVFQTLMTYLAYYYTDICKL
                     DTTDASAIMLTVGLIAAFGFNPIIGALADRTVSRWGKFRPWILWTAVPLGVVAFLAFS
                     QPDYEKYQSKVIYAVITYTLLLLLYAANNLPYSALSGVITGDMKERNSMSAYRFVAVM
                     FAQFFVQVFMLPIIEHVGHGSKAAGMKEVMTWLAIIGTIMLVITFLSTKERIVPKPEQ
                     KSSLKEDLGDLVKNKPWVIMLLLTTLVFITLAMKGGSYVYYFKNYVDKDSLTQFIQPI
                     TSFLSDIGMNFFGEDPVSAGFGLFNAGGIIFMIVGITLSKGLADKYGKRDVFGVALFI
                     STLFILFFFFFPPKSVALMFTSQILHGFFYGITIPLLWAMIADVADYSEWKNNRRATA
                     IIFSAMMVGLKVGLSVGAALLTRILGFYDYVANSDASQTPQAVNGTKMLVSVFPAIPF
                     LIGVALLFFYAINKKMEVRIEGDLLDRRK"
     gene            3168..4205
                     /locus_tag="PLU11_01035"
     CDS             3168..4205
                     /locus_tag="PLU11_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPN57717.1"
                     /translation="MPEDSIDHINFEELNRRAISQPLVSHIYTADPSAHVFNGKIYIY
                     PSHDIDAGDAFDDLGSHFAMEDYHVLSMESPEDKSATDHGTALHVKDIAWAEKQLWAP
                     DAAEKDGKYYLFFPAKGYDGIFRIGIAVSDSPTGPFIPQPEPIKNSFSIDPAVFKDDD
                     GTYYMYFGGIWGGQLQRWRNGIFNGEQPESPFAFLPEADEPALCAKVAKLSDDLLEFA
                     EPTKDLVIVDNAGNPLLQGDTNRRFFEASWMHKHDGKYYFSWSTGDTHYLCYATGDSP
                     YGPFTYQGRILEPVLGWTSHHSICAWKDQWYLFYHDSSLSKGVTHLRSVKMTAIQYDK
                     DGRIIPIRPYL"
     gene            4291..6903
                     /locus_tag="PLU11_01040"
     CDS             4291..6903
                     /locus_tag="PLU11_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPN57718.1"
                     /translation="MKKLFLVLAIHLGTTLLAQDYKTFPMWNPALPAEQRAKDVISRL
                     TLEEKIAQMLNATPAVSRLGIPAYDWWNEVLHGVARTPYKVTAYPQAIGMAATWDSNS
                     LKRMADFSALEGRAIHNKSIELNKTGDRYVGLTYWTPNINIFRDPRWGRGQETYGEDP
                     FLTAMMGKAFVNGLQGNDPRYLKAAACAKHFAVHSGPEPSRHSDNFNPSAYDLWDTYL
                     PAFRELVTKAHVAGVMCAYNAVDKQPCCANDQMMNDILRKQWKFTGYVTSDCWAVDDF
                     FRYHKTHKDATSSAIDAVIHGTDLECGNSVYRTLIDAVKSGQIKESYIDLSLERLFII
                     RFRLGMFDPVSMVKYAQTPASVLEAPAHKAHALKMAQQSIVLLKNENNTLPLSKKLKK
                     VVILGPNADNKIALLGNYNGTPSHVTTILEGIKAKLGKDVEVVYEKAINFTNDTLFVK
                     NTGKNAFFHEGKPGVKADYFLNEKLEGAPALTRMEDDINNFWTEGEPFAPGMPWTHYS
                     ARYTTSITAEKDEVLNFESEGNDGYRIKINDSTVVDVWERNRWGNKQFSFPVKKGQTY
                     TIEMTFRQSEGDAIVKLRAGHYEKTDFTELARRYSDADIILFAGGISPQLEGEEMPVT
                     VPGFKGGDRTSIQLPTVQTELMKALKATGKPVVFVMLTGSAIAIPWENDNIPAIINAW
                     YGGQSGGTAVADVLFGDYNPAGRLPVTFYKSDSDLPGFSDYSMKGRTYRYFNGEALYP
                     FGYGLSYTRFKYNVTQVPAVLTKGKPVTVAFTLTNTGKKDGEEVAQLYITHQSTGLKP
                     IRALKGFQRVFLKAGETKKLMFTLTSDQLSLVNETGALYQPTGKISISVGGGQPGVSN
                     KTSSNIVTKDITVQ"
     gene            6911..8836
                     /locus_tag="PLU11_01045"
     CDS             6911..8836
                     /locus_tag="PLU11_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPN57719.1"
                     /translation="MKKSLLLLSVLVFLNESNAQVRLPRLIRDSMILQRDSKVTIWGW
                     ASANEKIAVKLNNKTYKTKADAAGNWNLILSPMKAGGPYTMDITGKNKISLKEILFGD
                     VWFCSGQSNMVHQLNIHDVTYANEIANANFPQIRQFWIPTLTNLQGPQADLPAGNWKP
                     AVGEEVRPFSAVAYFFAKKLFEKYQIPIGIINASVGGTPIEAWTSEEGFSDFASVSAI
                     IQKNKDTAYINSFNRRSGPPRTRAQTDLGLTGSLKWHDPSYSPKGWRTINIPGYWEDQ
                     GIKDLNGVVWYRKEIDIPATMTGKAAKLFLGRIVDADELYINGKLVGNTTYQYPQRRY
                     KLPEGILKPGKNLFVIRVTNTGGKGGFVPDKPYCLFAGTDTVDLKGTWHYKVGDVFPP
                     FTGGGGGGGLSFQNQPTALYNAMVAPGIKYTIKGFCWYQGESNAGKPDEYAKLLPALI
                     RDWRTKWNQGPLPFLNVQLPGFMDYNYLPVESNWAAMRESQLKALSVENTAMVVAIDL
                     GEWNDIHPDNKKTVGDRLALAAFKTAYGENSIHSGPIAQEVSADGNKLIIRFTHIGAG
                     LMTSDGEEPAEFAIAGADKKFVWARVRIEGDKIIVWNDQVTQPLYVRYAWADNPVNPN
                     LFNKEGLPASPFQLSIR"
     gene            8881..9879
                     /locus_tag="PLU11_01050"
     CDS             8881..9879
                     /locus_tag="PLU11_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN57720.1"
                     /translation="MKKSGIALLGLGYYATEQLAPAFRETQHCYLAAVITGEQSKGDH
                     FIREYGLSPQQVYTYDQFDQLKNDPSIEIIYIVLPHHLHAEYTIKALEAGKHVICEKP
                     MAATMKEAEQMMDAARRTGKTISIGYRLHFDPYHREMIRLVREQVFGKLTGIDCSFSF
                     IPPKAVWRLSKEASGGGPLVDVGIYCIQAVCYLTGQEPVAVTAQSLPVTDGEKFNDIE
                     ETLLFQLEMPDGLIANCRTSFSETSSYLRIDAEKGWAELNPAFNYNGLVMKKSDGQET
                     IYPPCPQQALQMDAIAQAIQIGQPSSVPGSMGVRDMKIIDAIYKAMESGNRVQVNY"
     gene            9907..11625
                     /locus_tag="PLU11_01055"
     CDS             9907..11625
                     /locus_tag="PLU11_01055"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HPN57721.1"
                     /translation="MSQKLRSHDWFGRKDKDGIIYRSWMKNQGMPPDWFDGRPVIGIC
                     NTYSELTPCNAHFRDFAESIKKGIIEAGGFPVEFPIMSLGETLLKPTAMLFRNLASMD
                     AEESIRGNPIDGVVLMTGCDKTTPSTVMGAASVGLPTIVVPGGPMLNGRYKGQTIGSG
                     THVWKFDEDMKTGKMTQEECEFAESCMSRSIGHCMTMGTASTMACMVEALGLTLSGAS
                     AIPAADSRKKVMAQLSGRRIVEMVKENLTIDKILTREAFENAIKVNAAVGGSSNFIIH
                     LLAIAGRIGIDLKLEDFDVLGSHIPLLLNLMPSGKFLMEDFYYAGGLPVVLHELKEQL
                     HKNVVTVTGKNHHDNIQGNTDCYNREVIASFNQPLIPEAGCVIVKGNLAENGAVIKPS
                     AATPALMIHTGRAVVFESIEDYHKRIDDPELDIDENCVMVLKYVGPVGYPGMPEVGNM
                     ALPKKILEKGIRDMVRISDGRMSGTAYGTAVLHVSPESAIGGTLALVQNGDLIELNVP
                     ERRLQLLVSEVELAVRKAAWEAPPAAANRGYVHLYINHVLGADKGADLDFLQGHSGSA
                     VTRDSH"
     gene            11647..12660
                     /locus_tag="PLU11_01060"
     CDS             11647..12660
                     /locus_tag="PLU11_01060"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPN57722.1"
                     /translation="MKKIAPLLVYLALMTGCNTDTKEKSGYPTTGYIERLDPGLDSLI
                     IKEAKIEIIATGLDWSEGPLWVEKENMLLFSDVPRDTIFKWTETGGKEVYLTPSGYTD
                     TIKRGGEIGSNGLLLDPSGNLVLCQCGNRQMARMDAPLNKPEPLYTPLATRYDGKRFS
                     SPNDAVYNAAGELFFTDPPYGLESQRDDDPKKEMKWNGVYKVKTNGEVILLTDSIPRP
                     NGIALLPGDQQLIIACSDPAKPNWYIYDLAGDSLMNGRIFHSTLNELKEVNGLPDGLK
                     IGKNGIMYASGPGGIWIFTSTGKLLGRIRVKNPASNCALSGDEKTLYITNDMQILRVK
                     LRN"
     gene            12715..14022
                     /locus_tag="PLU11_01065"
     CDS             12715..14022
                     /locus_tag="PLU11_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HPN57723.1"
                     /translation="MTILIILFAIGLQVFLTVKKLSPFLSLLIVAIVAGLLLGMSPAD
                     LVKSIEKGAGSTLGGLALILCLGAVLGKILEASGAAEKIALTLIQLFGEKYIQWAILL
                     TGFLIGIPLYYNAGFVILVPLVFMLARKTGLPLLYIAIPMAASLSTTHCFLPPHPGPV
                     VLVNAFKANTGLVLLYGIMIAIPAVILAGPLLGKWFKQGKNISAGKNEMDTTKQVDLP
                     AALPSFSIALLPVLLITLSVSGQHFLEEGILRTSLQFLGDSNIALLLSVLIALFTFGY
                     RKKINMTVQMKWVSDGISGIALILLIITAGGIFKQVLQDSGTGEYIASFSSRWNMPPL
                     LFAWVITALLRVTIGSATVAGITAAGIVSPLLATGTVSPELMVLAVGSGSVFGSHIND
                     SGFWMFKEFFNLTLKQTFLSWTLMETIISLAGLAGVLIINQFI"
     gene            14040..15083
                     /locus_tag="PLU11_01070"
     CDS             14040..15083
                     /locus_tag="PLU11_01070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPN57724.1"
                     /translation="MSIAKAYAAQDKTSLLAPWQFERRNPGPHDVQIDIHFCGVCHSD
                     LHQVRDEWGGSIYPMVPGHEIVGKVAAVGTAVTKFKVGDTAAVGVMVDSCRTCKNCEK
                     QMEQYCEEGMTGTYNGYERDKKTMTQGGYSSAIVTDERWVYHVSDKLDMAATAPLLCA
                     GITTYSPLRFAGVKKGMKVGIIGLGGLGHMGVKFAVSFGAEVTLISTSPSKQKDAERL
                     GAHHFLLSTDKESMKKFKGYFDVLLDCVSANHDYNNYLNLLDLEGKLMVVGLPSESPE
                     VSPFALITNRRTITGSMIGGTAETQEMLDYCAEHNIVSDVEVIPVQQINEAYERMLKN
                     DVRYRFVLDMKTL"
     gene            complement(15164..17863)
                     /locus_tag="PLU11_01075"
     CDS             complement(15164..17863)
                     /locus_tag="PLU11_01075"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN57725.1"
                     /translation="MICYKKIQSTIFLVTLSVFTSFGQQFRVQGTATQTGVGIYRITA
                     DATGQVGMLTNYYSLDLLSNFTISFQLNFGTKDDTGADGMAFMMSRSCNPSLTQGGGL
                     GVQGIPNSLIIDFDTYANGLPDDLAADHTGIYANGNMTNTGLIMDALTSPVCLTDLCP
                     NVEDGQWHTVQVQWEYLTASSQRISLFFDGQLRGSSTRNHIADEFANATSVYWSVAGS
                     TGALSNLQQMRVTDYTNIFTYCEGTVYTLTAPPNGSNYSWTGNSSVTNVASYTAIASG
                     TITCTYTNNCGQPESVSFGINVPFLVTPVLTSTMNACAGTDAVFTIASTPGYRIRYSL
                     NSGPVLELIIDASGVATITIPAINTDQTLQLTQVYDGTCGKDINLLEAVLVRPNPIVS
                     ASGGALCSATTIQLSGTPSGGTWSGAHISTAGVFDAATATPGFHNVTYQYTNQWSCSN
                     SIITQVEVYARPVVQAQNGSVCAGRAVQLQATPAGGNWSGNHISASGIFDATGVLAGL
                     YPVTYSYTDGNNCSNQSLANVVVNPLPVLTLTDAEVCSGSGIQLIALPTGGIWTGAHI
                     SSNGSFDATGLAAGIYPVQYSFSDQNGCNATGNSNIRVFSDQIIPLLTASAAICEGED
                     AHFQLSGIPGALIAYSINGSQPVQVILNAAGTAEVIVTGAVNNQQLLISQVESGPCIK
                     TIDVQATVPVKLKQEVRISREICAGDLFEGYGTAGTFTDIFTGVNGCDSTRVLDLSIR
                     QYIKPDLGPDQVFCEGDTLRITPGDFSSYLWSTGETTSSILVTGQGLYSVATGTVCGS
                     ATDQVLVGPGLCDIYFPSGFTPNGDGRNDEFKPETNLRPLTYNMQVFNRWGQLIFTSS
                     DIRNGWDGTFGGKRVDSGVFVYHASYSIRGVHKTIRGMITLIR"
     gene            18073..18443
                     /gene="ssrA"
                     /locus_tag="PLU11_01080"
     tmRNA           18073..18443
                     /gene="ssrA"
                     /locus_tag="PLU11_01080"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18584..20383
                     /locus_tag="PLU11_01085"
     CDS             18584..20383
                     /locus_tag="PLU11_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57726.1"
                     /translation="MNHQSPADYYSQRIQTLNDSLHSLKQKALTLSWLRLFSFLATIA
                     AGWLLWPEGLIPAFSGVIAGLVFFIIFVNRDLDNKTAIENTSRLLAICRNEQLALEHH
                     FYQFPDGASYMDTQHPYANDLDLFGHASLFQYINRTESEQAGRLLADWLLEPASAETI
                     LRRQKAVDALSDIPEWNEQLQSHGAAKKITLSAELHICQWMEEQNNVRTHMGWKLTRY
                     LFPLASLTWLLLYIVDQIPQSLFLTGLTIFLAISYAITKKLIPAWNKLGKISAELESF
                     SDSIRHIETASFNNELLNAHKATFSNPPLPSASTQIKSLKKIMDRLDYRLNPVVFIPL
                     SVFLCWDLQQVFALEKWKAANKAGIQHWFASLAEMEALSSLGRLRFNHPEWVFPVLET
                     ATIFAGTSIGHPLIPAGKRVNNDFNTQGDGIINLVTGSNMAGKSTFLRSCGVNIILAM
                     TGAPVCATGLRLSPLRVMSSMRISDNLEESTSTFYAELKKLKAIIEAVNKKEQVFLLL
                     DEILRGTNSGDRHTGSKALIKQLIAQHATGIVASHDLELAKLKEVYPQQLFNYHFDVQ
                     IANEELYFDYTLKEGVCQSMNASLLMKKIGINM"
     gene            complement(20543..21916)
                     /gene="rseP"
                     /locus_tag="PLU11_01090"
     CDS             complement(20543..21916)
                     /gene="rseP"
                     /locus_tag="PLU11_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPN57727.1"
                     /translation="MMSLLAINWAVVGIKAGQLILSLSILVILHEFGHYITARWFKCR
                     VEKFFLFFDPWFSLVKRKVGDTVYGIGWLPLGGYVKIAGMIDESMDKEALKQPPQEWE
                     FRSKPAWQRLIVMLGGVIMNILTAFVIYAFILMIWGDKKVPSSSMKYGVHVGDSTLMK
                     IGFKNGDRIIGVDGKEVYDLTRFKKKLITAEKVQIERDGQLIILDLPKDFVGQLIENK
                     NQKRKGFIGPRFPAIVANVQDTSIAYQSGLRKNDRIVALDTVRFEYFDQLSDILDTKA
                     NKTVLLTVNRNGVDTSFNTKINGEGQIGFNPYSSMEQLDSLGWLKLEITRYSFFAAFP
                     AGVKMTYTELRDYIDQFRTIFTPGTGGIKGIGGFKSMGSIFAPTWDWESFWRLTAMLS
                     VILAFMNILPIPALDGGHVMFTLYEMITRRKPNEKFLEYAQVVGMVLLLGLMLYANGN
                     DWFGWGR"
     gene            complement(21968..23137)
                     /locus_tag="PLU11_01095"
     CDS             complement(21968..23137)
                     /locus_tag="PLU11_01095"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPN57728.1"
                     /translation="MNKEIPVKRIAIFGSTGSIGTQALDVIAANTDKFSAEVLTAQNN
                     DELLIRQALQFNPNVVVIGDEKKYNRVKEALASTDIKVFAGEKALEEVASMDCYDLML
                     AAIVGYAGLKPTLNAIYAGKAIALANKETLVVAGDIIMQKAVEHRVPIIPVDSEHSAI
                     FQCLVGETRNRIDKIILTASGGPFIGRKPNYLVNVKREHALQHPNWSMGAKITIDSAT
                     LMNKGLEMIEAKWLFNLQPEQIQVVIHPQSIIHSMVQFEDGSIKAQMGLPDMKLPIQY
                     AMAFPKRIPNDFPRYDFKKVSTLTFEEPDLKTFRNLALAQDALAKGGNLPCIMNAANE
                     IAVFAFLRNRINFLDMTEVIEKTMQHLPFIEKPTLEEYFESDGEARNFAADIIKL"
     gene            complement(23145..24644)
                     /locus_tag="PLU11_01100"
     CDS             complement(23145..24644)
                     /locus_tag="PLU11_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPN57729.1"
                     /translation="MKIKSFLAKPFAAYIYKGIRKGMATALADQDAIFKQLVKTGRLT
                     EFGKAGGLDQVNDYEGFKQSVPIRDYEQLKPWIERIKEGKHNVLWKGKPIYFAKTSGT
                     TSGTKYIPITKDSIPNHINTARNALLCYMAETGNTRFADGKLIFLSGSPVLDRIGGIP
                     TGRLSGIVNHHVPQYLRSNQMPSYETNCIEDWETKLGKIVDETINQNMTLISGIPPWM
                     QMYFDELVRRSGKKVGELFPNFNVMVQGGVNFEPYKAKLFESIGRKIDTIELFPASEG
                     FFAFQDSQEAEGLLLNTNSGIFFEFVPAGEIFNENPTRLSLKDVKVGENYALIINNNA
                     GLWGYNIGDTVKFLSVDPYRLIVSGRTKHFISAFGEHVIGEEVEYSMLKVAEEMNVQI
                     TEFTVAPFVSSDAGKSYHEWFVEFEQPPVNIDEFAGRLDLALRGKNVYYDDLIGGSIL
                     KPLKLTPVRKNGFIDYMKSIGKLGGQNKVPRLSNDRKIADELIKWTVNH"
     gene            24802..25101
                     /gene="rplU"
                     /locus_tag="PLU11_01105"
     CDS             24802..25101
                     /gene="rplU"
                     /locus_tag="PLU11_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPN57730.1"
                     /translation="MIAVVKVAGQQYKVEKDQTLYVPRVEGNAGDKLDLEVLLVDADG
                     KLSVGSAVGTKVQAEILDQVKGDTVIAYKQKRRKGFHKKKGHRTAYTKIKVTSIA"
     gene            25179..25439
                     /gene="rpmA"
                     /locus_tag="PLU11_01110"
     CDS             25179..25439
                     /gene="rpmA"
                     /locus_tag="PLU11_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPN57731.1"
                     /translation="MAHKKGEGSVKNGRDSHSKRLGVKIFGGQPAVSGNIIVRQRGTV
                     YHPGKNVGVGRDFTLFALTDGVVEFRKAKNNKTVVSVALAEA"
     gene            25550..25672
                     /locus_tag="PLU11_01115"
     CDS             25550..25672
                     /locus_tag="PLU11_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57732.1"
                     /translation="MATKKKAAKKAAPKKAAKKAAPKKAAKKAAPKKKAAKKKK"
     gene            25939..>27523
                     /locus_tag="PLU11_01120"
     CDS             25939..>27523
                     /locus_tag="PLU11_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPN57733.1"
                     /translation="MDNAAIADNFSLLAKLMDIHGENSFKTKTYGAAGFNIEKMPVSL
                     KDTPREKLFGIKGIGPSVGEKVIEMLDTGKLNILEEYIANTPPGVIEMLNIKGIGPKK
                     IHTIWKEMGIESIGELLYACNENRLTLFKGFGEKTQQNVQAAIEFYFQNQGHFLYAQL
                     DEIFPQIDQYLKKIFGTDIVHSTGRYRRQELTIEELEFVVLRPNEKVKPAFQTAQPPE
                     LLEEKADSLLYQLKNGLKLRLYTGGDTMAESLFKTTASPEFMEAFENAFPVIDYKKAG
                     ADEAAVFRQAGIPVIPPCLRETGDIIRTARQQGIPKLIQASDIRSLIHSHSNWSDGSN
                     TLEEMAAECIKRGWEYMVISDHSQTAFYANGLKEDRIREQHRQIDLLNSQLAPFRIFK
                     SIESDILNDGSLDYPEEVLKTFDLVIASVHSNLGMSEEKANARLIKAIENPYTRILGH
                     MTGRLLLSRNGYPVDHRLIIDACEANKVVIELNAHPRRLDIDWRWISYALEKEVLLSI
                     NPDAHALEGFNDVKYGVLAA"
BASE COUNT         7692 a         6444 c         6295 g         7092 t
ORIGIN      
        1 agagtcagta tgtctccatc ggggcagatc attatacaaa agcggtcaac agcaacggaa
       61 tcaaatggac tgtattaccg gatctgggcc gcaccggttc tgcaatcacc acttacccgg
      121 taacggctcc accgcagcaa cccggtggaa atgccccgca taccgaatat gaattctaca
      181 catacagcag tggtgatttc aggataagtg ccttcttttc gcccacactc aactttcata
      241 atactgcatc aggtctgcaa tatgccattt ccgtagatgg agaagcaccc cggatcatca
      301 gcatcaataa tgatgaaaga actacagaag gcgggatcaa ttataaatgg gtgggtgata
      361 atatcattat taaaacatct tcccaccaaa ttaagcaggc tggaaaacac accctgaaat
      421 actggttggt gaacagtggt ttggtgctgc aaaagctggt gctggatttt ggaggactat
      481 tacccagtta tctaggaccg gaagaaacaa tcaataaaat tgtaaataaa taaccatgaa
      541 accaattaca ttagtcctgt tgatagttgt ctcaggtatt attacaaccg gttgtgcatc
      601 cggtaaaaag acaacgaatg tttccgatag tattacattg aaggatgcat ttaagaatga
      661 tttcgtgatt ggtgcggctg taaataccgg ccagatcaat gaacgggatt ccggctctgc
      721 agcacttctc cgcacccatt ttaacgggct cacacccgag aatattatga aatgtgagat
      781 tatacacccg gaatgggacc gttatgattt tacactggcc gacaaactgg tggagcttgg
      841 caagaaatac aacagccctg tatatggaca taccctgatc tggcacagcc aattgtcgcc
      901 ctttattcag aaaattcagc gtgtggattc attccggact ttctttacca atcatattac
      961 caccattgct tcccggtacg atggaaaatt gaaatcctgg gatgtggtaa atgaggcact
     1021 caacgaagac ggcacgttgc gcaaatccgt cttcctgaac aaactgggtg aggattatat
     1081 agttgaagca ttccggctgg cacagaaagc ggccccgaat acggaattgt attataatga
     1141 ttataatatt gagcagccaa agaaaagagc cggagccatt gccatcatta aaaaaattca
     1201 ggcggccggg gttcggatcg acggagttgg tatacaggga cactggcgct cttataatgt
     1261 gcccatgaaa gaaatcgaag agagtataaa ggagtttgct gcactgggga ttaaagtgca
     1321 ctttacagag ctggacctcg gggtgttacc gaatccatgg gacaatgatg ctgccgatgt
     1381 gaatatgaaa gctgaatatt ctgctaaaat gaatccgtac gcagccggat taccggattc
     1441 agtaagtgca aagcaggcac aaagttatgc tgacctgttc cgccttttcc tgaaataccg
     1501 gaaagacatt gcccgaatca ctttctgggg cgtaaatgac gggcaatcct ggctgaacgg
     1561 atggccgatc cgtggacgga ccaattaccc gctgttgttt gaccgaaata ataaacccaa
     1621 agatgctttt tataaagtaa ttgagaccaa agccaaccca gtaaaagata aaaaatcatt
     1681 ctaacgaaac cgctgccaac atcatgtcaa ctacatcaca aaaattatcg gtccgcgaaa
     1741 aaatcggtta cagtttaggc gacctcgccg ccaaccttgt tttccagacc ctgatgacct
     1801 acctggccta ttactatacg gatatctgta aattggatac cacggatgct tccgcgatta
     1861 tgctgactgt cggattgatt gccgcattcg ggttcaatcc gattatcggg gcactggcag
     1921 acagaactgt ttcccgctgg ggtaaattca gaccctggat tctctggact gctgtaccat
     1981 tgggcgtggt cgccttcctg gctttctcac aacccgatta tgagaaatat caatccaaag
     2041 tgatttatgc agtgatcacg tataccttgt tacttttatt atatgcagcg aataacctcc
     2101 cctactccgc cctcagcggc gtgatcaccg gggatatgaa agaacgcaac agtatgtcgg
     2161 cctaccgttt cgtggctgtg atgttcgccc agttttttgt acaggtattc atgttgccta
     2221 tcattgaaca tgtgggtcat ggcagtaaag ccgcaggaat gaaagaagta atgacctggc
     2281 tggctatcat tggaacaatt atgcttgtga tcacgttcct gtccacaaaa gaacgcatcg
     2341 ttccaaaacc ggaacagaaa tccagtctga aagaagacct tggcgacctg gtgaaaaaca
     2401 aaccttgggt gattatgcta ctgcttacca cgttggtgtt tatcaccctg gcaatgaagg
     2461 gaggttctta tgtttactat tttaaaaatt atgtggataa ggatagcctg acacagttca
     2521 ttcagccaat aacatctttc ctgtctgaca ttgggatgaa cttcttcggg gaagacccgg
     2581 tatccgccgg attcggacta tttaatgccg ggggtattat ttttatgatt gtggggatta
     2641 cgctctcaaa gggattggct gacaaatacg ggaaacgaga tgtatttggt gtagcccttt
     2701 tcatttccac cctgttcata ctctttttct ttttcttccc cccaaaatca gtcgcactga
     2761 tgttcacctc ccagattctg catggctttt tttatggcat caccattccc ctactctggg
     2821 ctatgattgc cgatgtggcg gattacagtg agtggaaaaa taaccgtcgg gccactgcta
     2881 ttatcttttc ggccatgatg gtgggcctga aagtcgggtt gagtgtcggt gccgccctgt
     2941 tgacacgcat ccttgggttc tatgattacg tggccaacag tgatgcttca caaacacccc
     3001 aggcggtcaa tggcaccaaa atgctggtga gtgtctttcc tgctatcccc ttcctgatcg
     3061 gggtagccct gcttttcttt tatgcgatca ataaaaaaat ggaagtccgg attgaagggg
     3121 acctgctcga caggagaaaa taattctgct tattcaaaca gttttttatg ccagaagaca
     3181 gtatcgatca tatcaatttt gaagaattaa accgccgtgc tatttctcag ccactggtca
     3241 gtcatattta tactgcggat ccttccgcgc atgtattcaa tggcaaaatt tatatctacc
     3301 cgtcacatga catagatgcg ggcgacgctt ttgatgacct cggcagtcat tttgccatgg
     3361 aggattatca tgttttatcc atggaatcac cggaagacaa atccgcaacg gatcatggaa
     3421 cagcccttca tgtcaaggat attgcatggg ccgaaaaaca actgtgggct ccggatgccg
     3481 cggagaaaga cggaaaatat tatctcttct ttcctgcaaa aggatatgac ggtatctttc
     3541 gtatcgggat agcagtcagc gactcaccca ccggtccgtt tatacctcaa ccggaaccta
     3601 tcaaaaacag cttttccatt gaccctgccg tatttaaaga tgacgacggg acttactata
     3661 tgtatttcgg gggtatctgg ggtggacagt tgcaacggtg gagaaacggc attttcaacg
     3721 gagaacaacc ggaaagcccg tttgcctttt taccggaagc agatgaaccg gctctatgcg
     3781 ccaaagtggc aaaactatcc gacgatttac ttgaatttgc tgaacctaca aaagatcttg
     3841 tcattgtgga caatgcaggc aacccgcttt tgcagggtga tactaaccgg cgcttttttg
     3901 aagccagctg gatgcacaaa cacgatggga aatattattt ctcctggtcc accggggata
     3961 cccattatct ctgttatgcc accggggatt ccccctatgg tccgtttacc tatcagggcc
     4021 gcatcctgga accggtgctg ggttggacct cacaccattc catctgtgcc tggaaagatc
     4081 aatggtacct tttttatcat gactccagct tgtcaaaagg agtcactcat ttacgttcgg
     4141 taaaaatgac cgctatccag tatgataaag acggcaggat tatccccatt cgtccttatc
     4201 tgtaaacaag tataaaatca gggacgcttc cggtaaaata tccggatatt tatgaataga
     4261 aactgaaaca acattcatca atataaacat atgaaaaaac tattcctcgt tttggccatc
     4321 cacctgggta caaccctcct tgcccaggac tataagactt tcccgatgtg gaacccggcc
     4381 ctgccggctg agcaacgggc caaagacgtg atcagccggc tgacgctgga agagaaaata
     4441 gcccagatgc tgaatgccac gccagccgta tcacggttag gcatacccgc atatgactgg
     4501 tggaatgaag tattacacgg cgtggcccgt accccgtata aagtgacggc ctatccgcag
     4561 gccatcggca tggccgctac ctgggacagt aattcattaa aacggatggc tgatttctct
     4621 gcgttggaag gacgagcgat tcataataaa tccattgaac tgaataaaac cggcgaccgg
     4681 tatgtgggac ttacctactg gacccccaat atcaatatct tccgcgatcc ccgttggggc
     4741 cgtggacaag aaacctatgg cgaggatcct tttctgaccg ccatgatggg aaaggccttt
     4801 gtaaatggat tacagggcaa tgatccacgc tatctgaaag ccgctgcctg cgccaaacac
     4861 tttgcggtac acagcggacc ggaaccctcc cgtcactctg ataattttaa tcccagcgcc
     4921 tacgatctct gggataccta tctgcccgct ttccgggaac tggtaacaaa ggctcatgta
     4981 gcgggtgtaa tgtgtgcgta taatgcagta gataaacaac cctgctgcgc caatgaccag
     5041 atgatgaatg atatccttcg taaacaatgg aaattcaccg gctacgtaac cagcgactgt
     5101 tgggcggtgg atgatttttt ccggtatcat aaaacccata aggacgccac cagctcagcc
     5161 attgacgcgg tgattcatgg aaccgacctg gaatgtggta attcagtata ccgtaccctg
     5221 attgatgccg taaaaagcgg acaaatcaaa gaatcctata ttgatctttc tctggaacgt
     5281 ttatttatca ttcgtttccg gctgggtatg tttgacccgg tatcgatggt gaaatatgcg
     5341 caaaccccgg cttccgtatt ggaagcccct gctcataaag cgcatgcctt aaaaatggcg
     5401 caacaatcca ttgtcttact gaagaatgaa aacaataccc tgccactgag taaaaaactg
     5461 aaaaaagttg taatccttgg ccccaatgca gacaataaaa ttgctttact cggtaattac
     5521 aacggcaccc cttctcatgt gaccaccata ctggaaggaa taaaagcaaa actgggcaag
     5581 gatgttgaag tggtatatga aaaagcgatc aattttacta atgatacctt gtttgtaaaa
     5641 aacaccggta aaaatgcatt tttccatgaa ggtaaaccgg gagtaaaggc agactatttc
     5701 ctgaatgaaa aactggaagg cgctccggcg cttacccgga tggaagatga tatcaataat
     5761 ttctggacag aaggtgaacc ctttgcaccc ggcatgccct ggacccatta ttcagcccgt
     5821 tatactacaa gtataaccgc cgaaaaagac gaggtactca attttgaatc tgaaggaaat
     5881 gacggttacc gtattaaaat caatgacagt actgtagttg atgtatggga aagaaaccgc
     5941 tggggtaata aacaattcag tttcccggtt aaaaaaggtc agacctatac cattgaaatg
     6001 acgttccgcc agagtgaagg tgacgccatt gtcaaactcc gcgcaggcca ttatgaaaaa
     6061 acagatttta cagaacttgc ccgcagatac agtgatgccg acatcattct ctttgccggt
     6121 ggtatttccc cgcaacttga aggagaagaa atgccggtta ccgttccagg ctttaaagga
     6181 ggggaccgca ccagtatcca gttaccaact gttcagactg aattaatgaa agcccttaaa
     6241 gccacaggta aaccagtggt gtttgtcatg ctaaccggca gtgctatcgc cataccctgg
     6301 gaaaatgaca atatcccggc cattatcaat gcctggtatg gcggtcaaag cgggggtacc
     6361 gcggtggcgg atgtactttt tggggattac aatcctgccg gcagattacc ggttacattt
     6421 tataagtcgg acagcgacct gccaggtttc agcgattatt ccatgaaagg aagaacctat
     6481 cgctacttta acggggaagc cctctaccct tttggttatg gattgagtta tacccgtttt
     6541 aaatacaatg taactcaagt accagctgtg ctgacaaaag gaaaacccgt taccgtggct
     6601 ttcacgctga ctaataccgg taaaaaagac ggggaggaag tggcccagtt atatatcacc
     6661 catcaaagca caggtctcaa acctatccgg gcattgaaag gttttcaacg ggtattcctg
     6721 aaagccggtg aaaccaaaaa actgatgttt acgcttacat ccgatcaact ttccctggta
     6781 aatgaaacag gcgccctgta tcaacccacc ggaaaaataa gtatcagtgt cggtggaggt
     6841 caacccggtg tatcgaataa aaccagcagt aatatcgtaa caaaagatat tactgtacaa
     6901 taatttgagc atgaaaaaaa gtctgctgct actttcggtt ttagtttttc tgaacgaaag
     6961 taatgcccag gttcgtttgc cccggctgat ccgtgacagt atgatcttac agcgggacag
     7021 taaagtaact atatggggtt gggcttccgc caatgaaaaa atagcggtaa agctcaataa
     7081 caaaacttat aaaaccaaag cggatgctgc tggtaactgg aacctgatcc tttctcccat
     7141 gaaagccgga ggtccttaca ccatggacat caccggtaaa aacaagatca gcctgaaaga
     7201 aattctgttc ggggatgttt ggttttgcag cggccaaagc aatatggtac atcaactgaa
     7261 tatccatgat gttacttatg ccaatgaaat tgccaatgcc aattttccgc agatccgcca
     7321 gttctggatt cccaccctga ccaacctgca ggggccacaa gcagatctgc cggccggcaa
     7381 ctggaagccg gctgtaggcg aagaggtgag gccgttttca gcggtagcct attttttcgc
     7441 taaaaaatta tttgaaaagt atcagatccc tataggtatc attaatgcga gtgtgggagg
     7501 tacaccgatt gaagcgtgga ccagcgaaga aggattcagc gattttgctt ccgtcagtgc
     7561 catcattcaa aaaaataaag acactgctta cattaatagt ttcaaccgga gatccggtcc
     7621 tccaagaaca agagcccaga ctgacctggg cctgacgggc agtttaaaat ggcatgaccc
     7681 gtcctattca cccaagggct ggcggacgat caatatcccc ggttattggg aagaccaggg
     7741 tattaaggac ctcaatgggg tggtctggta ccgcaaagaa atagatatcc cggctaccat
     7801 gaccggtaaa gcagccaagc tatttcttgg aaggattgtg gatgcggatg aactctatat
     7861 caatgggaaa ctggtgggga ataccactta ccaatatccg caacgccggt ataaattgcc
     7921 ggagggaatt ttaaaaccag gaaagaatct ctttgtcatt cgtgtaacca atacgggagg
     7981 aaaaggaggc tttgttcccg ataaacctta ttgccttttt gccggaacag acactgtcga
     8041 tctgaaagga acctggcact ataaagtggg cgatgtattt cctcctttta ccggaggcgg
     8101 tggcggaggc ggactttcct tccagaacca accaaccgcc ctgtataatg ccatggtggc
     8161 tccgggaatt aagtatacaa ttaaaggttt ctgctggtac cagggggaaa gtaatgcggg
     8221 taaaccagat gaatatgcga aattactgcc ggccctgatc cgggactggc gtacaaaatg
     8281 gaaccaggga cctctcccct ttctgaatgt gcagttaccc ggatttatgg attacaatta
     8341 cctgccggtg gagagtaact gggcggctat gcgggaatct cagctgaaag ctctctccgt
     8401 tgaaaacacg gcgatggtcg ttgccattga cctgggggaa tggaatgata ttcatcctga
     8461 caataaaaaa accgtaggtg atcgcctggc attggcggcg tttaaaacag cctatggaga
     8521 aaacagtatt cattccggcc ccattgccca ggaagtatcc gctgatggaa ataaactgat
     8581 catccggttt acccatattg gcgctggctt aatgaccagt gacggtgaag aaccggctga
     8641 atttgcgatt gctggagctg ataagaaatt tgtctgggcc agggtcagga ttgaaggaga
     8701 caaaatcatc gtatggaatg accaggtgac acaaccactc tatgtacgct atgcatgggc
     8761 cgacaatccg gtgaatccga atttatttaa caaagaaggc ttacccgctt ctccttttca
     8821 attaagtatc agataatata aattacacag tctgcattcc gctttttaat cctgttatac
     8881 atgaaaaaat caggtatcgc cttattaggt cttggctatt atgccacgga acaactggca
     8941 ccggctttcc gggaaacaca gcattgttac ctggcagccg tcattacagg ggaacaaagc
     9001 aaaggggatc atttcatcag ggaatatgga ttgtccccgc aacaggtata tacgtatgat
     9061 cagtttgatc agctaaaaaa tgatccctcc atcgaaatta tctatatagt actcccgcat
     9121 cacctgcatg cagaatatac catcaaagcc ctggaagcag gcaagcatgt gatctgtgaa
     9181 aaaccaatgg ccgctactat gaaagaagcg gagcaaatga tggacgccgc ccggagaacc
     9241 ggcaaaacca tttcaatcgg atatcggttg catttcgatc cctatcatcg tgaaatgatc
     9301 cgcttggtca gggaacaggt attcgggaaa ctgaccggca ttgattgctc tttctccttt
     9361 atccctccca aagccgtatg gcgactaagt aaagaagcat ccggtggagg accattggtg
     9421 gatgtcggta tttactgtat tcaggctgtt tgttatctta cagggcagga acctgtagcc
     9481 gtaactgcgc aatccttacc ggtaactgac ggcgaaaaat tcaatgatat tgaagaaacc
     9541 ctgttgttcc agctggaaat gccggatgga ttaatagcga actgtcgtac cagtttcagt
     9601 gaaaccagca gttatttgcg tattgatgct gaaaaaggtt gggctgaact caatcctgct
     9661 tttaattaca acgggctcgt catgaagaaa tcggatggcc aggaaaccat ctacccgcct
     9721 tgtccgcaac aggccctgca gatggatgcc attgcacagg cgatacagat cgggcaacct
     9781 tcatctgtac ccggatcaat gggggtcagg gatatgaaaa ttattgatgc catttacaaa
     9841 gccatggaat cgggcaaccg ggttcaggtt aattattaat ttcctcctca ttttaaaaaa
     9901 attaccatgt cacaaaaact ccgcagtcat gattggtttg gccgaaaaga caaggacggt
     9961 atcatctaca gaagctggat gaagaaccag ggtatgcctc cggattggtt tgatggccgc
    10021 ccggttattg gtatttgcaa tacctatagt gagctcactc cctgcaatgc ccatttcagg
    10081 gattttgcag aaagcataaa aaaggggatc attgaagccg gtggtttccc ggtggaattc
    10141 cctattatga gtcttggtga aaccttactc aaacctacgg ccatgctatt ccgcaacctc
    10201 gccagtatgg atgccgaaga atccatccgg ggcaatccga ttgatggcgt ggtactcatg
    10261 accggttgtg ataaaaccac cccctctacc gtaatgggag ccgccagtgt gggattacca
    10321 acgattgtgg tacctggtgg tccgatgctc aatggacgat acaaaggtca aaccattggc
    10381 agtggtaccc atgtctggaa gtttgatgaa gacatgaaga ccggtaaaat gacccaggag
    10441 gaatgtgagt ttgctgaaag ttgcatgagc cgcagtatcg gccattgtat gacgatgggt
    10501 accgcctcta ccatggcctg tatggtggaa gcattggggc ttaccctcag cggtgcatcc
    10561 gctattcccg cagcggactc ccggaaaaaa gtaatggccc agctcagcgg aagacgcatt
    10621 gtggagatgg taaaagaaaa cctgaccatt gataagatat taacccgtga ggcttttgaa
    10681 aacgcgataa aagtaaatgc cgctgtaggt ggttcttcca atttcattat ccacttactg
    10741 gcgattgccg gacgtattgg tattgatctg aagctggaag attttgatgt actgggcagt
    10801 catatccctc ttctgttaaa cctgatgccc tccggcaaat tcctgatgga agatttttat
    10861 tatgccggcg gcttaccggt ggtcctgcat gaactgaaag aacaattaca taaaaatgtc
    10921 gtgacggtaa ccggcaaaaa tcaccatgac aatatccagg ggaatacaga ctgttataac
    10981 cgtgaagtca tcgcctcctt taaccagccc ctgattcccg aagcgggttg tgtgattgta
    11041 aaagggaacc tggctgaaaa cggggcggtg atcaaaccat ccgctgccac cccggccctg
    11101 atgatacata ccggccgtgc ggtggtattt gaaagtattg aggactacca caaacgcatt
    11161 gatgatcctg aactggatat tgatgaaaac tgtgtgatgg tattaaaata tgtgggacct
    11221 gttggctatc caggtatgcc ggaagtgggc aatatggctt taccgaaaaa aattctggag
    11281 aaaggcatcc gcgacatggt gcgtatcagc gacggacgta tgagtggtac cgcctatggt
    11341 actgctgtac ttcatgtatc tccggaaagt gcaattggtg gcacactggc cctggtacag
    11401 aatggagatc tgatcgaatt gaatgtaccc gaacgccggt tgcaattatt agtcagtgaa
    11461 gtagaactgg ccgtcagaaa agccgcctgg gaggctcctc cggctgctgc taacaggggg
    11521 tatgtgcatc tttacattaa tcatgtactc ggtgcagaca aaggagctga tcttgacttc
    11581 ctgcaaggac attccggatc ggccgttacc cgtgattccc attgattaac ccatagcttc
    11641 gccattatga aaaaaatcgc ccccctcctg gtttacctgg cattgatgac cggttgcaat
    11701 actgatacaa aagaaaaatc aggctacccg acgaccggtt acattgaaag actggatccc
    11761 ggtctggata gcctcattat aaaggaggct aaaattgaaa tcattgctac cggcctggat
    11821 tggagtgaag gcccgttgtg ggtggagaaa gaaaatatgc tgctcttctc cgatgtgcca
    11881 cgtgatacca tattcaaatg gacagaaacc ggaggaaaag aagtatacct cactccctcc
    11941 ggctatactg atacgataaa gagaggagga gaaataggat ccaatgggct tttactggat
    12001 ccttcaggaa atctggtact ctgtcagtgc ggcaaccgtc agatggcacg gatggatgcc
    12061 cccttaaata aacctgaacc cctttataca ccacttgcaa cccgttatga tggtaaacga
    12121 ttcagcagtc ctaatgacgc cgtttataat gcagccggtg aattattttt cactgatccg
    12181 ccttatggac tggaatcaca acgggatgat gaccccaaaa aagaaatgaa atggaatggt
    12241 gtctataaag taaaaacaaa cggggaagtc attctgttaa cagatagtat accgagaccc
    12301 aatgggattg ctttacttcc cggagaccag cagctgatta ttgcctgttc ggatccggca
    12361 aaacccaact ggtatattta tgaccttgct ggtgattcat tgatgaatgg ccgcattttt
    12421 cacagcacac tcaatgaatt aaaagaagtg aatgggttgc cagacgggtt aaaaatcggg
    12481 aagaacggaa tcatgtatgc aagcggtccc gggggtatct ggatctttac ctcaaccgga
    12541 aaattactgg gaagaatccg ggttaagaat cccgccagca attgtgcatt gagcggggat
    12601 gaaaaaacct tgtatattac caatgatatg caaatcctgc gggtaaaact gcggaactga
    12661 tcaaacattc tctgaccatt caattacggt tgtacaaata atcagccagg ccatatgact
    12721 atactgatca ttctgtttgc catcgggttg caggtcttcc tgacggtaaa aaaactaagc
    12781 ccgtttttat ccttactgat cgtagccatt gtagcgggac tactgttagg catgtctcct
    12841 gctgacctgg ttaaatcaat cgaaaaagga gcaggcagca ccttaggtgg tctggccctg
    12901 atcctttgcc ttggcgcggt actggggaaa attctggaag ccagcggggc cgctgaaaaa
    12961 atcgcattga ccctgataca actatttggt gaaaaatata tccaatgggc catcctgctc
    13021 acgggtttcc tgatcggcat tccactctac tataatgcgg gttttgtgat cctggtaccg
    13081 ctggtattta tgctggctcg aaaaacaggc ttaccgcttt tgtacatcgc tatccccatg
    13141 gcagcctctt taagtaccac ccattgcttc ctgccaccgc acccgggacc ggttgtcctg
    13201 gtcaatgcct ttaaagcaaa taccggtctg gtattgttat acggaatcat gatcgctatt
    13261 ccggcggtca tactggctgg ccctttactg ggcaaatggt ttaaacaggg aaaaaacata
    13321 tcggctggga aaaatgaaat ggacaccacc aaacaggtgg atctccctgc cgcccttccc
    13381 tccttttcta ttgcgctttt accggtttta ttaattacgc tttcggtaag cggacaacat
    13441 tttttggaag aggggatcct gagaaccagc ttgcaattct taggggattc aaatatagcc
    13501 ctgttgctct cggtgctgat agccctgttc acgtttggct accggaaaaa gataaacatg
    13561 acggtgcaga tgaagtgggt cagtgacggt atttcaggaa tcgccctgat cctgttgatc
    13621 attacggcag gtgggatatt caaacaggta ttgcaggaca gtggcacggg tgaatatatc
    13681 gcttctttca gcagccgatg gaatatgcca ccgctcttat ttgcctgggt gataactgcc
    13741 ttgttacggg taaccatcgg atcagccacc gtagccggta ttacagcggc tggtattgta
    13801 tcccccttgc tggctaccgg aacggtttca cctgaactaa tggtcctcgc agtgggaagt
    13861 ggatctgtgt ttggatcaca tatcaatgat tcaggtttct ggatgttcaa ggaatttttt
    13921 aatctgactt taaaacaaac atttttatcc tggacactga tggaaacgat aatttcgctt
    13981 gccggcctcg ccggtgtgct gatcatcaac caatttatat aacgaaataa caaaatacca
    14041 tgtcaattgc aaaagcatat gccgcgcagg acaagaccag tctgttagct ccctggcagt
    14101 ttgaaagaag aaatcccggc ccacatgatg tgcagatcga tatccatttc tgcggggttt
    14161 gtcatagtga tctgcaccag gtaagagatg aatggggtgg ttctatttat ccgatggtac
    14221 ccggacatga gatcgtagga aaagttgcgg ccgttggcac agccgtcacc aaattcaagg
    14281 ttggggatac agctgcagtc ggtgtgatgg tagatagttg ccggacctgt aagaactgtg
    14341 aaaagcaaat ggagcagtat tgtgaagaag gcatgaccgg cacctataat ggctacgaac
    14401 gggataaaaa aaccatgaca cagggcggct attcctcggc cattgtaact gatgaacgat
    14461 gggtatacca tgtgtctgac aaactggata tggcggctac agcacccctg ctttgtgcag
    14521 gtataaccac ttactctcca ctccgttttg ccggggtaaa gaaaggaatg aaagtgggta
    14581 tcatcgggct tggtggttta ggacatatgg gcgtaaaatt cgccgtttca tttggcgctg
    14641 aagtaacgct aatcagcact tctccttcca aacaaaaaga tgcggaacgg ctgggagccc
    14701 atcattttct attgagcact gacaaagaat ccatgaaaaa atttaagggc tattttgatg
    14761 tactgctgga ttgcgtttca gctaatcatg attataataa ctatttaaac ctgctggatc
    14821 ttgaagggaa gctgatggtg gtgggtctac catcggaaag tccggaagtc agtccgtttg
    14881 ccttgatcac taaccgtcgg acaattaccg gctcgatgat cggcggcacc gcagagaccc
    14941 aggaaatgct ggactactgt gctgaacata atattgtatc tgacgtggag gtgattccgg
    15001 tacagcagat caatgaagcc tatgagcgca tgttaaaaaa tgatgtgcgg tatcgctttg
    15061 tactggatat gaaaacgctc taaagcagta atacacgctc cctttgtcaa tgcgttttgt
    15121 aacgaattga caaaggtatt tttataagaa tcatgacata cacttaacgt attaatgtaa
    15181 tcattccccg gatcgtcttg tggactcccc tgatcgagta tgacgcatga tagacaaata
    15241 caccgctatc gacccgtttc ccaccaaaag taccatccca tccattccgg atatccgaac
    15301 tggtgaatat caactggccc caacggttaa agacctgcat attgtatgta aggggccgga
    15361 gattggtttc cggtttaaac tcatcatttc tcccatcgcc atttggcgta aagccggaag
    15421 gaaaataaat atcacaaagc ccggggccga ccagcacctg gtctgtagct gatccgcaaa
    15481 cggttcctgt agctactgaa tacaaaccct gtcctgttac aagaatggat gacgttgttt
    15541 caccggtact ccataaataa ctactgaaat caccgggggt aatacgtagc gtatcccctt
    15601 cacaaaatac ctggtccggc ccaagatcag gttttatata ttgtctgatc gataaatcca
    15661 gtacccgggt actgtcacaa ccattcacgc cggtgaaaat atcagtaaac gttcctgctg
    15721 ttccatatcc ttcaaataaa tcacccgcac agatttcgcg gctgatcctt acttcttgtt
    15781 ttaatttaac aggaactgtg gcctgtacat cgatggtttt gatacatggc ccggattcaa
    15841 cctggctgat gagcagctgc tggttattga cagcccctgt cacgatcact tctgcagttc
    15901 cggctgcatt gagtataacc tgcacaggct gacttccgtt aattgaatag gcaatcaaag
    15961 cccccggaat accactcagt tgaaaatgcg catcttcccc ttcacaaata gcagcagaag
    16021 cagtcaacaa cgggataatc tggtcagaaa aaacccggat attgctgtta ccggtagcat
    16081 tgcaaccatt ctgatcacta aaactatact gaaccggata gatgccggct gcaagacccg
    16141 tggcatcaaa actgccattt gaagaaatat gagcaccggt ccatataccg cctgtaggta
    16201 aagcaatcag ttgtataccc gacccgctgc aaacctccgc atcggtgagg gttaaaaccg
    16261 gaagcgggtt gacgacaaca ttagccaatg actgattact gcaattattc ccgtcggtat
    16321 atgaataggt taccggatac aaacccgcca atacgcctgt tgcatcaaat atcccggatg
    16381 ctgaaatatg attccctgac cagttgccac ctgcaggtgt ggcctgtaac tgaaccgccc
    16441 ggccggcaca aacactcccg ttctgcgcct gcaccaccgg tcgtgcatat acttctacct
    16501 gcgttataat gctgttgctg caactccatt ggttcgtgta ctggtaagtt acattatgga
    16561 agccaggtgt tgccgtagcc gcatcaaaga cgcctgctgt tgaaatatgc gcgccgctcc
    16621 aggttcctcc cgatggtgtg cctgaaagct gaatcgtggt agcggaacat aaggcaccgc
    16681 cactggctga cactattggg ttgggtctga ccagtaccgc ttcaagcagg ttgatatcct
    16741 tgccacatgt gccatcatat acctgcgtca gttgaagggt ctgatctgta ttgatagcgg
    16801 gaatagtaat ggtcgcgact ccggatgcat caataatcag ttccagaacg ggaccactgt
    16861 ttaatgagta tcgtatccga taacccgggg ttgaagctat cgtgaacacg gcatctgtac
    16921 ctgcacaggc attcatagtt gatgtgagta ccggtgtaac gagaaacggc acattgatcc
    16981 caaaacttac ggattccggc tgaccgcagt tgttggtata cgtacaggta atggttccgc
    17041 tggcaatggc ggtataactc gcgacattag taacagaact gtttcctgtc cagctataat
    17101 tcgatccgtt gggcggagcc gtaagtgtgt ataccgtacc ctcacaatag gtgaaaatat
    17161 tggtataatc cgtcacccgc atctgttgca ggttagacaa cgccccggtg cttccggcca
    17221 cggaccaata tacggaagtg gcatttgcaa attcatcggc aatatgattt cgggtggaac
    17281 tgccccgcaa ctgtccatca aaaaataagg agatacgctg actgcttgcc gtcaggtatt
    17341 cccattgcac ttgtacggta tgccattgcc cgtcttccac attcggacaa agatcagtga
    17401 gacataccgg tgaggtcagt gcatccatga tcaaaccggt attggtcatg ttcccgttgg
    17461 cataaatgcc cgtatgatcg gcagcgaggt catccggcag tccgttggca tacgtatcaa
    17521 aatcgatgat cagtgaatta ggaataccct gtacacctaa tccaccgccc tgtgtcagtg
    17581 atgggttgca ggaccgcgac atcataaagg ccatcccgtc cgccccggta tcatccttgg
    17641 tgccaaaatt gagctggaaa ctgatggtga aattactgag aaggtcaaga ctgtaatagt
    17701 ttgtcagcat ccccacctgc ccggtagcgt cggcggtaat ccgataaata ccaacgcctg
    17761 tttgtgtagc ggtaccttgt acacggaatt gctggccgaa gctggtaaaa actgataagg
    17821 taaccagaaa gattgtactc tgaatttttt tataacagat cataatgggt gaaaaacaac
    17881 gaggtaaaca aagtaaaggc aaatcagtcc agaaacaaaa tttcaccacc agcctggcag
    17941 gtttaattcc tactttgaca ttcctcatgc tgtttctgaa atccggctat cctgctatcc
    18001 attgaattcc agtcactcgc taacataaaa tccggaattt gaataaaaga ggtaaatttg
    18061 ctgtccctaa ccgggggcgc ttggtattga cagcgtagct ctttggaagt gtaagcatgt
    18121 actgcgttgc gtaattagca gtttaatctg aatacgaaaa actcaaatgg caatacacag
    18181 tatgccatgg ctgcctaatc agtgattaag tagtcattag ggccgccggg ccggttgatc
    18241 tgttaccatg ccccgccgcc gactcaaaaa caaaacaggt tgctgtaacc ccaggctgtg
    18301 caggttgctt aagactatcc agctaagaga taaatcggtt gttcattccc agtttatctc
    18361 cgacaatcca aaatgaaata aacatgtaga aagcttttgg agaatatgtt tggacaccgg
    18421 ttcgagtccg gtcgcctcca cctttgttcc tttagcattt gcgaatgggg attcgcccat
    18481 caggatattt ctcctgatgg gctttttaat tctgatcagt ctgaaactgc tttccctttt
    18541 ttaattttta tttttgaatt ttgactttac ctagttttac cccatgaacc atcagtcccc
    18601 agccgattat tattcacaac gaatacaaac gctaaatgac agccttcatt cacttaagca
    18661 aaaagcgcta accctttcct ggcttcgtct tttcagcttc ctggctacca tcgccgccgg
    18721 ctggctgctc tggcccgaag gtttaattcc tgcattttca ggcgttattg ccggactggt
    18781 attctttatc atttttgtca accgagatct cgataacaaa acagcgattg aaaatacgtc
    18841 gcgcctactg gccatctgcc ggaatgagca acttgccctg gaacatcatt tttaccagtt
    18901 tcccgatggg gcttcctata tggatacaca gcacccctat gcgaatgatc tggatttgtt
    18961 tgggcatgcc tccctttttc aatatatcaa ccgtactgaa tccgaacagg ctggacgttt
    19021 actggctgac tggctgctgg aacccgcttc agcagagacg atcctgagac gacagaaagc
    19081 agttgacgcc ctgtccgata ttcctgaatg gaacgaacaa ctccagtcac atggtgcggc
    19141 aaaaaaaata acattgtcag cagaacttca tatctgtcag tggatggaag aacaaaataa
    19201 tgtgcgcact catatgggct ggaagctaac ccgctatctt tttccgctgg ccagtttaac
    19261 ctggctatta ctttatatag tggatcagat tccgcaatcc ttattcctta ccgggttgac
    19321 cattttctta gctatttctt atgccatcac aaaaaaactg ataccggcct ggaataaact
    19381 gggtaagatc agtgcggaac tggagagttt ctcagatagt atccggcata ttgaaaccgc
    19441 cagctttaac aacgaattac tgaatgccca taaagccact tttagtaatc caccactacc
    19501 aagcgcatca acccaaatca aatcgctgaa gaagatcatg gaccggctgg attaccggct
    19561 caatccagtt gtattcattc ctctcagtgt atttctttgc tgggatctgc aacaggtatt
    19621 cgcccttgaa aaatggaaag cagcaaataa agcaggcatt caacattggt ttgcctctct
    19681 agctgaaatg gaagcccttt catccctggg acgattacgt tttaatcatc cggaatgggt
    19741 tttcccggta ctggaaacag ccaccatatt tgccggaact tcaattggtc atccgctgat
    19801 tcctgctggt aaaagagtga ataatgattt caatacacaa ggtgacggga tcatcaacct
    19861 ggttaccggt tccaatatgg ccggcaaaag tactttcctt cgcagctgtg gggtcaatat
    19921 catattggcc atgaccggtg ccccggtttg tgccactggc ctccggctct ctcccctgcg
    19981 tgtgatgagc agcatgcgga tcagcgacaa cctggaagaa agcacctcca ctttttatgc
    20041 ggaattgaaa aaacttaaag caattattga agccgttaat aaaaaagagc aggtattcct
    20101 gctgctggat gaaatcctcc gtggcaccaa ttccggcgac cggcataccg gctccaaagc
    20161 cctgatcaaa caactgatcg cccaacacgc caccggcatc gtggcatcac atgaccttga
    20221 gctggccaaa ctaaaagaag tatacccgca acaattattc aattaccact tcgatgtcca
    20281 gatcgccaat gaggaattgt attttgatta taccctgaag gaaggggttt gccagagcat
    20341 gaacgcgtcg ctgctgatga aaaagatcgg aatcaacatg taagtacgaa gtacgaggta
    20401 cgaagtacga atggcgtgat aggcgaaaat gtttactgaa ttgaaaacag tattaaaata
    20461 gtaaacccct atttatttag gataaacgcc cacagtatgg gctgttgact actaactccc
    20521 gactactaac tcccgactat acctacctgc cccatccaaa ccagtcattt ccattcgcat
    20581 aaagcatcag tcccagcagc agcaccattc ccaccacctg cgcatattcc aggaatttct
    20641 cattgggttt tctgcgtgtg atcatttcat aaagcgtgaa catcacatgt cctccatcaa
    20701 gggcaggtat tggtaaaata ttcatgaatg ccagaatcac ggaaagcatc gcggttaaac
    20761 gccagaaaga ttcccagtcc caggtggggg caaatatgga tcccatggat ttgaagccac
    20821 caatcccttt gatcccgccg gtacccggtg tgaaaatggt cctgaactgg tcaatataat
    20881 cgcgtagttc tgtataggtc attttgacac cggccgggaa cgcagcaaaa aaactatacc
    20941 tggtgatttc cagtttcaac caacccagac tatccagttg ctccatactg ctgtacgggt
    21001 taaagccgat ctggccttcc ccattgatct ttgtattgaa actggtatcc accccattcc
    21061 ggtttaccgt cagtaatacg gtcttgtttg ctttggtatc gagtatgtcg gataactggt
    21121 caaagtactc aaaccgtact gtatccagtg ctacaatccg gtcattttta cgcaacccgg
    21181 attggtaagc aattgaagta tcctgcacat ttgcaacgat ggccggaaaa cgcggtccga
    21241 taaatccttt tcgtttctgg ttcttatttt caatcagctg tcctacaaaa tccttaggca
    21301 ggtcgagtat gatcagttga ccatctcgtt caatctgaac tttctcagca gtaatcagtt
    21361 tctttttgaa acgggtcagg tcatacactt cttttccatc tacgcctata atccgatcgc
    21421 cattcttaaa tccgattttc atcagggtac tgtcgcccac atgcacgcca tacttcattg
    21481 aactggaagg cacttttttg tcgccccaga tcatcaggat aaatgcataa attacaaatg
    21541 cggtaagaat attcatgatc acaccaccca gcatcacgat cagccgctgc caggccggtt
    21601 tgctgcgaaa ttcccattcc tgcgggggtt gtttcagcgc ttccttatcc atgctctcat
    21661 cgatcattcc ggcaatcttt acatagccac ccaatggaag ccagccgata ccataaaccg
    21721 tatcgcccac ttttcgtttc accagggaaa accagggatc aaaaaacagg aagaattttt
    21781 caacccggca tttgaaccag cgggctgtga tataatgtcc aaactcgtga agaattacca
    21841 ggatcgacaa cgaaagaatt aattgtccgg ccttgatacc caccactgcc cagtttatcg
    21901 ctaaaagact catcatggaa ataaaaataa aatcccgaac aggatcggga ataaacaata
    21961 ggtttaatta taattttata atatccgctg caaaattacg ggcctccccg tcgctttcaa
    22021 aatattcctc cagcgtgggt ttttcaataa aaggcaggtg ttgcatggtt ttttcaatga
    22081 cttcggtcat atccaggaaa ttaatccggt tacgcaaaaa ggcaaaaacc gcaatttcat
    22141 tggccgcatt catgatacag ggaagatttc ctccttttgc cagtgcgtcc tgggctaaag
    22201 ccaggttacg gaaggtcttc aggtcaggct cttcaaaagt cagggtactt acttttttga
    22261 aatcatacct cggaaaatca tttggaatcc gtttcggaaa agccatggca tactggatcg
    22321 gcagtttcat gtccggcaat cccatttggg ctttgatgct gccatcttca aactgtacca
    22381 tactatggat gatgctctgc gggtgaatca ccacctgaat ctgttccggc tgcagattaa
    22441 acagccattt agcttcgatc atttccagcc ccttattcat cagcgtggcc gaatcgatgg
    22501 tgatctttgc ccccatactc cagttgggat gttgcagggc atgctcccgc tttacattca
    22561 ccaggtaatt gggtttccgg cctataaaag gaccacctga agcagtaaga atgattttat
    22621 caatccggtt acgggtctca cctaccaggc actggaagat ggcggaatgt tcagaatcga
    22681 ccggtatgat gggcacccga tgctctactg ctttctgcat gatgatatct ccggcgacta
    22741 ccagcgtttc tttattagcc agggcaatcg ctttgcccgc atagatcgca ttcagtgtcg
    22801 gttttaatcc ggcatatcct acaatcgccg ccagcatcag atcgtaacaa tccatggaag
    22861 ccacttcctc gagcgctttc tctccggcaa aaaccttgat atcagttgat gccaatgctt
    22921 cttttacccg gttgtatttt ttttcatccc cgatcaccac cacattgggg ttaaactgaa
    22981 gtgcctgacg gatcagcagt tcatcattat tctgggctgt cagcacttca gccgaaaact
    23041 tatcggtgtt ggccgctatc acatccagtg cctgggtacc aatggatccg gtggaaccaa
    23101 agatggcaat tcgtttaaca ggtatttctt tattcattaa cgacttaatg atttactgtc
    23161 catttaatta attcatccgc gattttccgg tcattactga gccggggtac tttattctgg
    23221 ccgcccaatt taccgattga tttcatgtaa tcaataaagc catttttcct gaccggggtc
    23281 agcttcagcg gtttcagtat cgatccgccg atcaggtcat cataatacac attttttccg
    23341 cgtaaagcga ggtccagtcg tccggcaaat tcatcaatat tgactggcgg ctgttcaaac
    23401 tccacgaacc actcatggta ggattttccg gcatcactgc tcacaaaagg agccacggtg
    23461 aattcggtga tctgcacatt catttcctcc gccactttga gcatactgta ttccacttct
    23521 tcaccgatca catgttcacc aaaagccgaa ataaaatgtt tggtccttcc ggatacgatc
    23581 agccggtaag gatctacgga cagaaacttc acggtatcac cgatattata gccccaaagt
    23641 ccggcattat tattaatgat cagtgcgtaa ttctcgccca ctttcacgtc tttcagcgat
    23701 aagcgggtcg gattttcatt aaagatctca ccagccggaa caaattcaaa aaagatgccg
    23761 ctattggtat tcagtaacaa tccttctgct tcctgtgaat cctggaatgc gaagaatcct
    23821 tcactggccg gaaataattc aatggtatca attttacgcc cgatgctttc aaagagtttg
    23881 gctttatagg gttcaaaatt caccccgccc tgtaccatta cattaaaatt aggaaagagt
    23941 tcacctactt ttttaccact ccggcggacc aattcatcaa aatacatctg catccaggga
    24001 ggtatcccgc tgatgagggt catattctgg ttaatggttt catccactat tttacccagt
    24061 ttggtttccc agtcttcgat acaattggtc tcataactgg gcatctggtt tgaccgcaga
    24121 tactggggca catggtgatt cacgatgcca ctcaaccggc cggtcggaat gccgccaatc
    24181 cggtcaagca ccggtgaacc ggataaaaag atcagttttc cgtctgcaaa ccgggtattt
    24241 ccggtctcgg ccatataaca aagcagggca ttacgggctg tattaatatg gttggggata
    24301 gaatccttgg tgatcggaat atacttagtg ccgctggtgg tgccggaggt tttggcgaaa
    24361 taaatgggtt tccccttcca gagcacattg tgctttccct ccttgatccg ttcaatccag
    24421 ggtttgagct gctcatagtc acggattggc acagactgct taaatccttc ataatcatta
    24481 acttgatcca aaccccctgc cttcccgaac tcggtaagcc ggccggtttt caccaattgc
    24541 ttaaaaatgg catcctggtc agccagggct gtagccatcc ctttccggat acctttatat
    24601 atatatgcgg caaatggttt tgccagaaaa gacttgattt tcatacattc acgatatgat
    24661 ccgccttggc ggggtacaaa tatagcagat gccccggcaa tacgggggga ctaaatgttc
    24721 gtaaaacttt gatttcccgt ttgccacttc aaaaattccc cttacctttg cctcccgaat
    24781 tttcgggata aattataacg tatgatagct gtagtaaaag tagccggaca acaatacaaa
    24841 gtggaaaaag accagacttt gtacgttccc cgcgtggaag gtaatgccgg tgataaactg
    24901 gacctcgaag tattactggt tgatgccgat ggcaaactgt ctgtaggcag cgcagtaggt
    24961 actaaagtac aggcagagat cctggaccag gtgaaaggcg ataccgtgat cgcttacaaa
    25021 caaaaacgcc gtaaaggttt ccacaaaaag aaaggacacc gtactgcgta tacaaaaatc
    25081 aaagtaacca gtattgctta atcagtaagg atggcaacct gtaaaggttg ctgttaaact
    25141 gatcaacgaa tcaactcaaa acttataaac ctaataagat ggcacataag aaaggtgaag
    25201 gtagtgtaaa gaacggccgc gactcgcaca gtaagcgtct tggcgttaaa atctttggtg
    25261 gtcagcctgc agtatcaggc aacatcatcg tacgtcagcg tggcaccgtt tatcatcctg
    25321 gtaaaaatgt aggcgtaggc agagacttta ctttattcgc actgactgac ggtgtggtgg
    25381 agttccgcaa agcaaaaaac aacaagaccg ttgtgtctgt tgccttagct gaagcataag
    25441 cgttttgggc caacttttga attttgtgga aaaaaaattt gggtagtata aaataattgt
    25501 tatttttact atcgttaacc cattctacta aacattaaat tcaaaaaaaa tggctactaa
    25561 gaaaaaagct gcaaagaaag ctgctcctaa aaaagctgct aagaaagctg ctcctaaaaa
    25621 agctgcaaag aaagctgctc ctaagaagaa agctgctaaa aaaaagaaat aagttcgctt
    25681 atctctttaa agaaatagaa agtccttcga aattcgaagg acttttttta tgcaactaag
    25741 gaattaaaaa gtcaaaagta aaaattaaaa agccttaaag tggggactgt cttaaatcaa
    25801 agggtcctat tggggggggg tggagaaaaa aagcctgagc attgcttttg ggtaagttct
    25861 gctttcggtt tttttacttt ttacttttta cttttgatgt gacagccaga aacaaagggg
    25921 aaatcctaaa ttcgccctat ggacaacgcc gccattgccg acaacttcag cctgctcgcc
    25981 aagctcatgg atatccatgg agaaaactcc tttaagacaa aaacctacgg ggctgccggt
    26041 ttcaatattg aaaaaatgcc ggtttcgctg aaagacacgc cccgggaaaa acttttcggg
    26101 atcaaaggca tcggccccag tgtgggtgag aaagtaattg aaatgctgga taccggtaaa
    26161 ctgaatatcc tggaagagta tatcgccaat acaccacccg gggtgattga aatgctgaac
    26221 atcaaaggca ttggaccgaa aaagattcat accatctgga aagaaatggg gatcgagtcg
    26281 atcggtgagc tgctgtatgc ctgtaatgaa aaccggctca cactcttcaa aggatttggc
    26341 gaaaagaccc agcagaatgt gcaggccgcc attgaatttt atttccagaa tcagggccat
    26401 tttctatacg cccaactgga tgaaattttc ccgcagatcg atcagtacct caaaaaaata
    26461 ttcggaaccg atatcgtaca tagtacagga cgctaccgcc ggcaggaact caccattgaa
    26521 gaactggaat ttgtggtatt gcgtcccaat gaaaaagtga aaccagcctt tcagaccgcc
    26581 cagcctcctg aactactcga agaaaaagca gatagccttt tatatcagct taagaacgga
    26641 cttaaactcc gcttgtatac cggtggagac acaatggctg agtcattatt caaaactacg
    26701 gccagccccg aattcatgga agcttttgaa aatgcctttc ccgtgatcga ttataaaaaa
    26761 gccggggcag atgaagcggc cgttttcagg caggccggta ttccggtaat cccaccctgc
    26821 ctgcgggaaa caggtgatat catccggact gcccgtcagc aaggaattcc caaactgata
    26881 caggccagtg atatccgctc actcatccat agtcacagta actggagtga tggcagcaac
    26941 accctcgaag aaatggccgc cgaatgtata aagcggggat gggaatacat ggttatatcg
    27001 gatcactcac aaactgcttt ttatgccaac ggacttaaag aagaccggat ccgtgaacag
    27061 catcgccaga tcgatctgct caacagtcag ctcgcccctt tccgcatttt taaaagcatc
    27121 gaaagcgata tcctgaatga tggtagtctt gattatccgg aagaagtatt gaaaaccttt
    27181 gatctcgtga tcgcttccgt acacagtaac ctcggcatga gcgaggagaa ggccaatgcg
    27241 cggctgatta aagccattga aaatccatac acccgaatcc ttggtcacat gaccgggcga
    27301 ctgctattga gccgtaatgg ctatccggtg gatcaccggc tcatcataga tgcctgtgag
    27361 gcaaacaagg tagtcattga actgaatgct cacccgcgcc ggcttgatat tgactggcgt
    27421 tggatctctt acgcccttga aaaagaggtc ctgctttcca ttaacccgga tgcacatgct
    27481 ctggaaggat ttaacgacgt gaaatatggt gtacttgccg cgc
//
LOCUS       DAOPMW010000010        27160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1556_length_27160_cov_8.187493, whole genome shotgun sequence.
ACCESSION   DAOPMW010000010 DAOPMW010000000
VERSION     DAOPMW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_27160_cov_8.187493"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..2992)
                     /locus_tag="PLU11_01125"
     CDS             complement(230..2992)
                     /locus_tag="PLU11_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN57734.1"
                     /translation="MRKALLLIFLTFISGLTLLAQQTVTGKITDTGGLPVAGVSIKAK
                     KSGKIALTGSDGTFTISLPAEDELEISSVGYIKQTVKTGSGSINISLVQSIEELGQVV
                     LVGSRRAGRVKTETTSPVDVINVSQVSGPTARMDLTSILNYTAPSFNYNKQTGSDGAD
                     HIDLATLRGLGPDQTLVLINGKRRHQTAFVAMFGTRGRGNTGTDLNAIPAGAIDRVEI
                     LRDGASAQYGSDAIAGVINIITKKTVNQITGSLGLSAYYDSKFNTAFESSLHKDYEHA
                     GKFDGKAFNGNINFGLPLGKKGGFINLTFDGTKTEKTFRQALKTDNYQQDDEAMYLNI
                     YRRGHGDAELEAFGSFVNLELPASSKTTFYVFGGTNKKNSDAFAFTRNWSARPDRFPT
                     NANGDLIVVPGIMKTASDGDTWYSPHIQTEIKDFAATGGLKGVVFNNWNWDLSNTTGN
                     NNFHFYGDKTFNASLGSAQQRFDDGGFSFLQNTSNLNFSREFAKNFNLGVGAEFRYEN
                     YKIFAGEEASYKNYDPTGDKATGSQGFPGYQPADEVNAKRRVFGAYADAEFDIQPTWL
                     MNLAVRLENYSDFGFTHNYKLATRIKASKTVNLRGSFSTGFRAPSLQQINFSSTFTTV
                     QAGNIAEVKIAPNYSNLAKAAGIPDLTQEKSISGSAGFTWKATSALNVTVDGYLTKIK
                     DRVVISGQFDGSDPNLDATLRSQMQGLNVSLAQFFANGVNTTNAGLDIVVEYNKKIGD
                     KRFRALLAGNLQTMKIDEINVPAKLSGSAFLQQTFLSDREQKFILASAPGMKGTLTLE
                     YGVKKLTLGTRLTYFGKVTLLGYGQDGLGISPTVPLDNGSGDVPDQFNYGGKLVSDIY
                     MSHPLRKKLTLYAGVDNLLNAHPDFGVAKGAKDWAYNTETGGPWDAVQMGTNGTRLFV
                     RIGFNF"
     gene            complement(3158..4357)
                     /locus_tag="PLU11_01130"
     CDS             complement(3158..4357)
                     /locus_tag="PLU11_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPN57735.1"
                     /translation="MLTISTRGIQMPPSPIRKLVPYAEAAKKKGIKVYHLNIGQPDIE
                     TPPAILDAVRNADIKVLEYSHSAGNESYRRKLVQYYKKVGIDISHDQILITTGGSEAI
                     MFGFFTCLNPGDEVIIPEPFYANYNGFACAAGVKVVPIPSSIDTGFALPPIADFEKVI
                     TPNTKAIIICSPNNPTGYLYSRTEMEAVKDICIKFNLYLFSDEAYREFCYDGEYVSAM
                     HLDGLDAHVVLMDTISKRYSACGARLGAFVTKNKQVYDAAMKFAQARLSPPGLAQIMG
                     EAAVDLPESYFDAPKAEYQRRRDLLVKRLNAMPGVFCPNPGGAFYAMAKLPIDDSDRF
                     CQWLLEEFSWNNQTVMLAPATGFYGTAGLGKDEVRLAYVLNLDAINGAMDCLEQALKV
                     YPGRTGS"
     gene            5059..5664
                     /locus_tag="PLU11_01135"
     CDS             5059..5664
                     /locus_tag="PLU11_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPN57736.1"
                     /translation="MPTGTDIQETDLKALFGSLEFFHPLSPEIKAYISHHIVTRQVSK
                     RILLLKEGETCHHVYFIRKGIVRGFFREGTKDITTWITAENELVSSISTWDIPRPVTE
                     NIQVLEDAELYVMTLAALKDLFIRYPEFNIVVRKLLQKYYADAENRAFIARLTKAENK
                     YRYFLKAHPSLANRVPLKYIASYLGMTLETLSRIRKKITRP"
     gene            6024..12716
                     /locus_tag="PLU11_01140"
     CDS             6024..12716
                     /locus_tag="PLU11_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57737.1"
                     /translation="MRRKKVNKILSVLLLLLFVVVISIFYITYRQSEDVRYNAQTVSF
                     TAEVISLSTAVELSSYDYILAVRDYINLQDPVLKVRINENQAKLTGYLNRLTLLEKND
                     IEQPSTIDSLSKYVTRLLRSAGFIDSNLFALPSLERDKMTAALKTEMRSQLGQINKYS
                     EVLKELEYMEREQQQNAEEKSLFWSKLILYAVLFIVLVLTAVIVFRIRTEFSRQSASE
                     QKFITLLEAAPDATIISNEQGVITLANRQAEKLFGYSMKELVGLEVELLIPKHMREAH
                     VSRRKGFTDQSRERGMGHGVEMIALRKDGIEIPVEISLSPIETKEGKLIAAAIRDITA
                     RKMAEAEMRQLYTQLNLAYEALYFTDEHLVIKTWNHGAEVLYGYTAEEAIGRYSVELL
                     KTDLTAEEYKEALTAMTNNDYWAGEVRRINKSGELVHVHSSLSAIRNEANEVTGYLSV
                     SFDISAEMKLREEVRKLANLTEQMSEAVMSRKAGSSEILTWNAGAERLFGFSKEEAVG
                     KSARELGILRLGPEVFQEIENALISTGEWKGEKCFFRKDGSSFTGAVSSNAIQNDQHD
                     RSYVFLVKDISTEKLLAAALKKNNEELEEKVQVRTLEIGQSEKQYRYLFENNPMPMWV
                     YDLDDFRFLDINEMAIHKYGYSREEFLKMTLMDIRPESDKERFLHFDHAWKMNSQDTN
                     RGVWKHCKKNGEIIEVEVIAHSILFQNKKAKLVLLNDVTNRLQTERKLVASEKKFRAL
                     IENSNDIIALLDADMKITYRSPSAIRITGWTNEEIKKMNFLDEIVHPDDRERLNELIR
                     SVLLSPGVPVVVYYRSLHNNGKYIDLEGVLINLMHRDYIRAMVFNLRDVTEQKKAEQL
                     LVAREKRFRSLIENSFDIILLLDENFKVAYRSPSAVRISGRSDAEVLGTDPRALIHGD
                     DLPMINQGIKRLMLAPGSSEHALFRYPHTDGTYLWMEGYATNLLQDENVRSIVFNYRD
                     VTARVEAEERLVNREKHFRALIENSMDVIVVLDEKFKVIYRSPSATKITGVSDEATIG
                     NDSRTNIHPDDLGSLNILLRELINLPGKSLNILFRYRRADGRYIWVEGSATNLLAEDN
                     IKGLVLNLRDVTDRILTEEKVRDSEERYRATLDNMMEGVQIIGFDWRYKYVNKAAAAQ
                     AHTTKDQLIGAGMTEKFPGIENTELYQLISRCFTERVPFQMENRFVLPDGEEIWTQLS
                     IQPVPEGVFILSVDITEKIIAAASLKEEKHKLESIAASSPGLIYSFQLKPDGSMSFPY
                     ASSAMEDMFGYGHELINQDVSALLSHTVKEDKEVFIERLTRSALQLTPWNLEFRFQHP
                     EKGLIWIEGNSIPTKEPDGTITWHGVIMDVTERKKIQDKIIEQAAQLRTLSDNLPGVM
                     IYQMVGKSFYERRFTYVSNEVTRLTGKTPEEVIADPYLLYNCILPEDIPLVSLAEEKS
                     FEQRTVFNEEVRFRTFNGEIKWLNIISTPRKTDQGDFVWDGFHVDITERKSAENVIRE
                     SEERYRTLVEQAFDGILFYDINGQILECNNSGANTMGYSREEMLQMNLADLFYPEDLE
                     IRPLNIQDVSSGMQIFDFRTMKKKDGTPVEVEWVTRLLPDGRMLGVGRDITHQLRALS
                     QQNLLSAIVNSSDDAIISTNLNSVISSWNLGAEKIYGYTAGEMKGNTVNVLIPPDTLN
                     EEAEILAKIMNGDMVIHYETIHVRKDGTKIFVSITTSPLLDSKGAVTGSSKIIRDITR
                     QKEDANRISQTNERYEAVAKATSDAIWDFDYETNQTFIAGTGYLDLFGYPLVNVFSEP
                     RFWEDRIHPEDYDKVMEAMEQAKRETTVSQSSLEYRFRRADGSWAYLYDRFFIIRDKE
                     GNPLRLLGAKQDITKRKEAEQELIQIAAEKQLLYERLSVILNTLPASVALLDPKGVMV
                     EVNDAWKNFADVNGFSGVNYGVGTSYFTVSTQSFGDNEQIGKDISAGVQEVLNGTLSQ
                     FEYEYSWHLTKLNRWFRIVVTPLKGREFNGVVVMHMDISEIRRLELERIESKIEEQKK
                     VTEAMLKGQEKERNAIGIELHDNVNQILVGTKVLLSVVRDFPEKTSELIPSCIDNLSL
                     AVQENRKIAHELVTPNLSDENLLKQITRLADTMLKNAGIQTYINSESLNEQLLSDEMK
                     LALYRVAQEQCTNIIKYAEAEQVIISLATKKDNFFMRIADDGHGTDPQKVTHGIGLKN
                     ISSRLGVFSGTISIDTAPGQGFALEIEIPLTPVKTPDLPD"
     gene            12803..14260
                     /locus_tag="PLU11_01145"
     CDS             12803..14260
                     /locus_tag="PLU11_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN57738.1"
                     /translation="MNRVNCTHSFRLIVWALVLFLHHYTASGQLCTGSLGDPVVHIDF
                     GSPSNPSTGFTAPGYTYSNSSCPNDGFYTITGYSTGCFGTAWHTVAADHTGGGNYMLV
                     NASYQPGDFFVKTVSNLCPNTTYEFSAWIMNVLISPTGIKPDITFRIETPSGTLLNQY
                     STGGISVTSSPRWEQYGFYFTTTAGNTDVVIRMTNNAPGGGGNDLALDDIMFRPCGPV
                     VTSVIQGVSLPVNVCVYEQQSYTLAGSLSPGFVNPVYQWQQSLDSGRTWTDIPGAASA
                     TYTRPPSGEGRFRYRLSVAESGNAGIASCRIASEQLEVNIHPRPQVSAGPDKIRVAGY
                     SDTRLTGMVSGDNVQYSWIPPDYLSSDTALQPVVTAVTDMQYLLTAVSGVGCTNEDAV
                     LVKVVAGVFVPSAFTPNNDGKNDRWTIPFLDPSWGARVMVFDRAGQLVYQARGVSVDW
                     DGNFKGMPLDAGSFVYFIQFADGKPDLKGTLTLIR"
     gene            14653..15930
                     /locus_tag="PLU11_01150"
     CDS             14653..15930
                     /locus_tag="PLU11_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPN57739.1"
                     /translation="MKMKFMALCLMAGLFVLPSCKKDKETTPTDPPVVVNPTLRAISP
                     AAGPKNTLVTISGTNFGTNSSVLKVYFNNVQASIQSVNDTLITALVPVNAGTGTVKLE
                     KNSTSVNGPAFEYYVTNRVLTYAGSSQGFADGNLAAARFNIVSGVVKDASGNLFVCDR
                     DNHRIRKITPQGVVSTFAGGTMGFADGTGSAARFNQPYSITIDGAGNLYVGDRINHSI
                     RKITTAGVVTTLAGNGTAGDATGTGSAARFNEPIGIVSDQAGTLYVADYINAKIRKVT
                     PAGVVTTYQNILAFGLAMDAAGNLYGTGYYNHFVYKYSPTGAYSVVAGQNGAAGNLDG
                     TGTGAQFNFPSGITVDANGNLYVCDAMNHRIRKITAAGVVTTLAGNDPGVMDGIGSGA
                     SFDTPLGICSDFANNAVFIADFNNSRIRKLIID"
     gene            15941..16141
                     /locus_tag="PLU11_01155"
     CDS             15941..16141
                     /locus_tag="PLU11_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57740.1"
                     /translation="MNKTEWWPKEGKLKKGLIILGIVIVAALIMTLIEEKGVTFTKRM
                     PFVIALSCLAVWFYQPAKKEKA"
     gene            16399..18243
                     /locus_tag="PLU11_01160"
     CDS             16399..18243
                     /locus_tag="PLU11_01160"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN57741.1"
                     /translation="MKSISFLFLFAAFLAIQPSAAQTCNCPEYSTLKEKWASSEDDIA
                     GYINQLRTSPGKLCKAKADEWMAVDFMENGKYDSSDLFFRKAEEKYRQTGCNDSVLVS
                     LYKNWAQLYYTNADFGKAQEYSFKLLKAAELSGNPHEMAVCYTMIAQLFNQTGQADKG
                     VVFTRKAIPLADKVTDPVKKSDLFYKIAKRYLWHFQDTKISSSLDSSELFTIRQLEVA
                     RSINRKSALAAGFSNLQGIAWERGDLRKALVLLDSSFTYTDKDEDDVVGTNYFDKADI
                     YVALKDYPKAALMADSALFYRLRDANPVYTAEVYELISRINLESGNYKGAYEFREKGR
                     AITDSVRNIEKIKEVSELEKKYSQEKNENKIKDLARQKQVYLLLAIAGLLGLAVLGFF
                     IRQQSLRNKQKILETEQRLNRARMNPHFFFNALTSLQLHALEENDGKTLASNISKFSH
                     IMRETLESTYKEYVTIAQEKDFLEEYMELQSIRIPDRFRFSVTYGTTIEPEDTIIPSM
                     ILQPFVENSIEHGFAGIDYAGQVDIHFDQAPGELLVRISDNGKGLLNGPKENNEHISR
                     ASQIIKDRIYLLNIKLRTKASFSIDNNAHEKGVTVLIRLPLLYKDQVR"
     gene            complement(18246..19844)
                     /locus_tag="PLU11_01165"
     CDS             complement(18246..19844)
                     /locus_tag="PLU11_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57742.1"
                     /translation="MKKALCSLLCLVLSISLLAQDPGYTGPAKMPLKNFWSHIEKLKA
                     GTGISSSVSNAERMLQQVKEKDPSYNTTAMEAELKIWKDKAAKESGDKNSAKAKEEAE
                     RNYFKSMYQKMVGVYSSGRDIQPGVTGKVYYDRVMALNLSEYKEKRAAAGTVDPKSYP
                     ALTDAVLADYNEYLKRSDRLKWNVVQALTSSKNIANPQEKMKLLEDIKYECEAVLILS
                     PNHPEFTQKLAEVKKLLGNADAEAAKFYTSDFHKEHVNQIVWSSQPLVIGKEKEMTSS
                     IKNEYKSGEAIFGTVYLGNNVNQLMKGNEQLRIIIRIDGGTAVWGGDLSNILLPLSIQ
                     NKSYLQFALLPDAKWLAANYAPYLADENWTYSYFFDDLVRSGDISHTISCELHFPTSI
                     INNIKSNVKLDLNEGTTAIKTQATKLHDELMAGRKLPKAGMSNASIEQQMVAAANNEG
                     WNDKFLKAIITSSSWNIEKNSLTSVIMYRYLSAVCTIKSPDGKCYYQEFTFRQDYAGN
                     GNYDSKIKFNSYGGKKEIGCDKLK"
     gene            20077..20823
                     /locus_tag="PLU11_01170"
     CDS             20077..20823
                     /locus_tag="PLU11_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN57743.1"
                     /translation="MKVLLIDDEANQRNAVKQLLRSFCPLVQTIEEASGVKSGLQAIR
                     DFQPDLVFLDVEMGDGTGFDLMREIRNPDFQVVFITAHNKYAITAFQYSAIDYLLKPV
                     DPAELKRAVDRAAVHVKNSSLQEQLQVLMQQLSGIHNQDRKIVLKDIDNTYFIKVSDV
                     LYCEAAGSYTRFYFTNQQPILVSRNLKEYEDVLEGLGFLRTHHSFLVNPEKIKLFDKT
                     DGGMLILEGGLSIPVSQRKKDFVMQVLENR"
     gene            complement(20895..21335)
                     /locus_tag="PLU11_01175"
     CDS             complement(20895..21335)
                     /locus_tag="PLU11_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPN57744.1"
                     /translation="MKKILFLATAFLFTVAVSAQTKPEELIKVNTENHDFGKVKQNVP
                     VSYYFEIKNISDKPVVVENTWASCGCTMPEKIVEPIQPGATVKLKVDYNSAAVGPINK
                     DVFIKLAGIDQAKSVKITGEVLTPEAYEAYLKGKGAIEKAPSKG"
     gene            complement(21347..21844)
                     /locus_tag="PLU11_01180"
     CDS             complement(21347..21844)
                     /locus_tag="PLU11_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPN57745.1"
                     /translation="MKKITLLLFCMTSLLALQAQQPVHNENDGHNHGSTAPQAATTEN
                     ADLLFKEMEYDFSKIPQGKPVYHFFEIVNKGKTPIKLDNIQASCGCTTPEWSRDPIAP
                     GASTKIKVGFNAASEGMFDKYITVFYNNSSQNKQIRIKGFVWKAPDGPAPANASVDFL
                     KKQTF"
     gene            21969..24362
                     /locus_tag="PLU11_01185"
     CDS             21969..24362
                     /locus_tag="PLU11_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPN57746.1"
                     /translation="MENSQSELLTDPGKLSYDRFREEVLNDYRIACISRETSLLGRKE
                     VLGGKAKFGIFGDGKELAQVAMAKFFQPGDFRSGYYRDQTFMFAIGLTTAEQVFAQLY
                     ADPDPSREPFSSGRQMNCHFSTRNVNEQGEWMELATTKNVASDMAPTAAQMPRSVGLA
                     YASRAFRNVEQLKAFRGLSDNGNEVCFTTIGDASTSEGHFWETMNAAAVLQVPMAVFV
                     WDDGYGISVPKKFQTVKASISDALEGMRKKEGTNGLNIYKVKGWDYAGMCEAFEEGIR
                     LARETHTPVLFHVEEITQPQGHSTSGSHERYKSPERLDWEREWDGIRKMKEWLIGNEL
                     ASEEELDEIAEKAKEHVRESKASAWAKFVTPIKKQVARATDLVHAMANALPEKSEELH
                     KVARELSANREPLRRDVQKGVHAALNIAGDHDAAFWTKDFYKELQEENKKYYNTHLYN
                     EGPKSALHVQEVHAVIPADAALLNGFEILNRYFDELFSSNPKVLAFGEDLGFIGDVNQ
                     GFSGLQQKHGAERIFDTGIRELTIMGQGVGLALRGLRPIAEIQYLDYLLYGLQPLSDD
                     VSTLHYRTGGHQSCPLIVRTRGHRLEGIWHSGSPMGMIINSLRGMYVCVPRNMTQAAG
                     MYNTLLRSNDPGLVIECLNGYRLKEKLPSNLMEFTVPLGVPEVLKEGSDITIVSYGST
                     LRIIHDAAHMLEAEGVSCEIIDVQTLLPFDIHHRILDSLKKTNRIVFVDEDVPGGAAA
                     YMFNKVMEEQGGYKFLDVAPRTITAKAHRPAYGSDGDYFSKPNAEEIQGVVREMMRE"
     gene            24805..25602
                     /locus_tag="PLU11_01190"
     CDS             24805..25602
                     /locus_tag="PLU11_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPN57747.1"
                     /translation="MKLTLYQIDAFTDNLFGGNPAAVIPLKKWLDTELMQKIALENNL
                     SETVFFVPSKMNGVDYDIRWFTPTLEINLCGHATLASSYVIFDILKSNKRKIVFHSQS
                     GMLTVKKQKGKMWMDFPSWKPERTADYPEALGQILGGAEVVGVYKHRDWLVELQNEEA
                     VLKCEPDFTLMKKHMDKLIITAPGKEFDFVSRFFAPAAGVSEDPVTGSAHSQLIPFWS
                     EKLGKHKMTARQLSARGGTLYCEQLSKERVLMGGTCVYYMKGTITLP"
     gene            complement(25599..26003)
                     /locus_tag="PLU11_01195"
     CDS             complement(25599..26003)
                     /locus_tag="PLU11_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HPN57748.1"
                     /translation="MAESTSLVLFDGVCNLCNSSVQFIIKRDKKDRFRFAPLQGKTGQ
                     AILAKHGLSADSLNSFVLSEGDKIYTHSTGALLMMKRLGFPWSIFYVYILIPRFIRDG
                     VYRWIARNRYRWFGRQESCMIPTPALKKKFLD"
     gene            26055..>27160
                     /locus_tag="PLU11_01200"
     CDS             26055..>27160
                     /locus_tag="PLU11_01200"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57749.1"
                     /translation="MNRYRLYFILLAFFYLPGLQLSAQSRQQDSLYVFDLINKADVFY
                     NGGQYDSALLYCRQAEDWSVRNQYKTGQAFALIKTGEIYIDKDELDKAAKSAEAVNKI
                     GLQQHDSLVTAIGWLQQGQVQLYSNQFDAAIKLYEKCLQYYLKQHPTRYSGLAYNDLG
                     YCWGRKGDLSRQAEYLFHSVRVYENYFPEEAGELGAAYNNISTVYYGLNDMNKAIEYA
                     KIAMKYREISGDAGLLSVSCCNISQYYISISKEESEKYLKLCEQYAVQSRDERRMIHS
                     YVTAYNFYYSNNRFAEAYQYELKSIAGLEKSGKNPAMLSRRYIGAAICANKLRMDSTL
                     ILGYFRKAKGMLDLLKDRINYRDYFWQLSDYYRNA"
BASE COUNT         7316 a         5843 c         6687 g         7314 t
ORIGIN      
        1 gattactcat tactcattct tctctctgga tgtttgctcc tttaagtctg ttcagtcagc
       61 tactcattac tgattactca ttactcattc ttctctctgg atgtttgctc ctttaagtct
      121 gttcaggcag ctactcatta ctgattactc attcttcatc tttaatgcct tttctataaa
      181 aaataagccg cttcaggaat gaagcggctt gaattatagc aggcaatcgt caaaagttaa
      241 atcctatccg gacaaagagg cgggtaccgt tggtgcccat ttgtacggca tcccagggac
      301 caccggtttc ggtattatac gcccagtctt ttgcaccctt tgctacccca aaatcggggt
      361 gtgcattcag caggttgtct acccctgcat acaaggtcag ttttttccgc aaaggatgac
      421 tcatataaat atccgagacc agttttcctc cataattaaa ctgatcgggt acatcgccgc
      481 tgccgttatc cagtggaaca gtgggtgaaa ttcccaggcc atcctgacca taacccagca
      541 gggttacttt tccaaaatag gtgagccggg tgcccagggt aagttttttc acgccatatt
      601 ccagggtcag tgttcctttc ataccaggtg cagaagctaa aataaatttc tgttcacggt
      661 cgctcaggaa ggtttgctgc aaaaaagcag agccgctgag tttggcagga acattgatct
      721 catcgatctt catggtctgc aggttgcctg ccagcaatgc acggaaacgt ttgtcaccga
      781 tttttttatt atactcgaca actatatcca gtccggcatt ggtggtattt actccattgg
      841 caaaaaactg ggccaggctc acattaagcc cctgcatttg tgaacgtaaa gtggcatcca
      901 ggtttggatc actgccgtca aactgaccac tgattaccac acgatctttg atttttgtta
      961 agtatccatc tactgtaacg ttcagggcgg aagtggcttt ccaggtaaac ccggcactgc
     1021 cactgatgct tttctcctgc gtgagatcag ggataccggc tgctttagcc agattactgt
     1081 aattgggtgc aatttttact tcggcaatat ttcctgcctg tacggtggta aaagtggagc
     1141 tgaaattgat ctgttgcaaa gaaggagccc tgaagccggt actgaaagat cctctgaggt
     1201 tgactgtttt agaggctttg atccgggtgg ccagtttata gttatgggtg aaaccgaaat
     1261 cactgtaatt ctccaggcgt accgccaggt tcatcaacca ggtgggttga atatcgaatt
     1321 ccgcatccgc ataggcgcca aatacccgtc gtttggcatt gacttcatcg gccggctggt
     1381 atccgggaaa accctgtgat cccgtggctt tgtcaccggt cggatcatag tttttataag
     1441 atgcttcttc accggcaaat attttgtaat tctcatacct gaattcggca cccacaccca
     1501 ggttgaaatt tttggcaaat tcacgactga agttcaggtt gctggtattt tgcaggaagg
     1561 agaaaccgcc gtcatcaaaa cgttgttgcg cggaacccaa tgaggcatta aatgttttat
     1621 cgccgtaaaa atgaaaatta ttattacctg ttgtattgct gagatcccag ttccagttat
     1681 tgaaaaccac tccttttaat ccgcctgtgg ccgcaaaatc ttttatctcc gtctggatat
     1741 gcggactata ccaggtatcc ccatcactgg cggttttcat aataccgggt acaacaatca
     1801 ggtcaccgtt ggcattggta gggaaacgat cggggcgggc cgaccagttt cgggtaaagg
     1861 caaacgcatc ggagtttttt ttgttggtac cgccaaatac atagaaagtg gtctttgacg
     1921 aagccgggag ttccaggtta acaaatgatc cgaaggcttc gagttcagca tctccatgtc
     1981 cacggcggta aatattaagg tacatggcct catcatcctg ctggtaattg tcggtcttta
     2041 aagcctgtcg gaatgttttt tcggttttgg tgccatcaaa ggtcaggttg ataaaccctc
     2101 ctttttttcc gaggggcagg ccgaaattga tatttccgtt aaaggctttg ccatcgaatt
     2161 ttccggcgtg ttcgtaatct ttgtgcaggc tgctttcaaa tgccgtattg aattttgagt
     2221 cataataggc agacaatccc agtgagccgg taatctggtt gactgttttt ttagttatga
     2281 tattgatcac cccggcaatc gcatcagaac catattgggc ggaagcgcca tctcttaaaa
     2341 tttcaacacg gtcaatggca cctgcaggta tagcgtttaa atccgtgcca gtattgccac
     2401 gtcctcttgt gccaaacatg gctacgaagg cggtctggtg acgtcgtttt ccattgatca
     2461 gcactaaggt ctgatccggg ccgagtccgc gaagcgtagc cagatcgata tgatcagccc
     2521 catcgcttcc cgtttgttta ttataattaa aagagggggc cgtataattc aatatggaag
     2581 tcaggtccat tctggccgtt ggtccgctta cctgcgaaac attgatcaca tccaccgggg
     2641 aggtggtttc tgtcttaacg cgacccgccc ggcgggaacc taccaaaacc acctggccca
     2701 gttcttcgat cgactgtacc aggctgatat taatggagcc gctgccggtt ttaacggttt
     2761 gttttatata cccgactgat gaaatttcaa gttcgtcttc agcaggtaaa ctaatcgtga
     2821 acgtgccatc cgaccccgtc agggctattt ttcctgattt ttttgctttg attgatacac
     2881 cagctaccgg cagtccgccc gtatctgtaa ttttaccggt taccgtttgt tgggcaagga
     2941 gggtaagtcc cgaaataaat gtcaggaaga taagcagaag tgcttttcgc ataattgttg
     3001 gttttgggtg gaatgtaagc aggtgaataa aagataactt aaatataatg gaaattatat
     3061 ttatataaaa tcattgacgg aggaatccct tgatttttag ccacaattta gccacgggca
     3121 ctgaccttac ccaacagcgc gaagaaagta gttccggtta ggaacctgtc ctgccaggat
     3181 atactttaag tgcctgttcc aggcaatcca tggccccgtt aatggcatcc agattcaata
     3241 cataagccag tcttacttca tcttttccca atccggctgt tccatagaaa ccggtggcag
     3301 gggccagcat aactgtttga ttattccagg aaaattcttc gagcagccac tggcagaacc
     3361 ggtcactgtc gtcgatgggg agtttggcca tggcataaaa ggcacctccg ggattcgggc
     3421 agaaaactcc aggcatcgca ttcaatcgtt tgaccaaaag atcacgtctg cgctgatatt
     3481 ctgccttggg tgcgtcgaaa taggattcgg gcagatcaac ggccgcttcc cccataatct
     3541 gggccagtcc gggaggactt agcctcgcct gggcgaattt catggccgca tcatatacct
     3601 gtttattctt tgtaacaaaa gccccgagcc gggcgccaca ggcactgtaa cgtttggaga
     3661 tggtatccat cagtaccaca tgggcatcga gtccatccag gtgcatggca cttacatatt
     3721 ccccgtcata acagaactcc cggtaggctt catcgctaaa caaatacagg ttgaatttga
     3781 tacatatatc ttttacagct tccatttcag tcctgctata gaggtagccg gtcgggttat
     3841 tggggctgca gataatgatt gcttttgtat taggggtgat cactttctca aaatcagcaa
     3901 taggagggag ggcgaaaccg gtgtcaatac tggaaggaat ggggaccact ttcaccccgg
     3961 cggcgcaggc aaagccgtta tagttggcat aaaagggttc gggaataatc acttcatcgc
     4021 caggattgag gcaggtgaaa aaaccaaaca taatggcttc gctgccaccg gtggtaatca
     4081 gtatctggtc atgactgata tcaatcccga cttttttata atactgcacc agtttacgcc
     4141 ggtagctttc attgcccgca ctgtggctgt attccagcac ttttatatcg gcgttccgga
     4201 cggcatccag gatagccggt ggtgtttcta tatctggttg cccgatattc aggtggtaga
     4261 cttttatgcc ttttttcttg gccgcttccg cgtaaggcac cagtttacgt ataggagagg
     4321 ggggcatttg tatcccccgg gtggaaattg taagcatata acaaaggtag ggggaatatt
     4381 aaatcagcac cctggaagat acagccaata cgatgcctaa atggcggata aaccgtgtat
     4441 tagcccgctt gccggggcag gaatcaagaa ttgaaaggtg cagcattttt ctgcttttgt
     4501 agttgtaata ctacttcatt ataagcaaag gggacgagca gatatccggc ggtttatagc
     4561 atattgataa cgttaacaag taaacgtaaa agggtagttt gaaggccgtt tgtttaattt
     4621 tcgatcgttt tttagtgtaa actcttagca agaatcctat gagtaataca taaaaaccct
     4681 caaaatttga tataaatcaa acggaaactt taaaacatgg cactcttatt gttatattta
     4741 cgttactaaa atctatcatt atgattaccg ttacaatttt agcctgggta ttgttttttg
     4801 cattcctggc aggttcagtt atgatgatca caagaggtac agataaggct taattcagac
     4861 tggaactagt agtccaattt gctcagagtt agtaaacgtt atttaagagg ttgtctattt
     4921 atgacagcct ctttttttat gccctttatg ttggtttttt tattcagcga ttcccctgat
     4981 tgactggtgc ctggttatca atcaggcgct ttccgttttt agttatattt gataattaca
     5041 ttacctattt acatcattat gccaaccgga actgatattc aggaaacgga tttgaaagcc
     5101 ctgttcggct cgctggagtt ttttcatcca cttagcccgg aaatcaaagc gtatatcagc
     5161 caccacattg taacccgtca ggtcagtaaa aggatattat tattgaaaga aggagagacc
     5221 tgccatcatg tttattttat ccggaaaggc attgtcaggg gctttttccg tgaaggcaca
     5281 aaagatatca ctacctggat cacggcagag aatgaattgg tttcttccat ttccacctgg
     5341 gatattccaa ggccggttac ggagaatata caggtgctgg aagacgcaga gttatatgta
     5401 atgaccctgg cggcgcttaa agatttattt attcgttacc cggagtttaa tattgtggtc
     5461 cgtaaactcc tgcagaaata ttatgcggat gcggaaaaca gggcatttat tgcgcggctc
     5521 actaaagcag aaaacaaata ccgctatttt cttaaagcac acccttcgct ggcgaaccgg
     5581 gtgcctctta aatatatcgc ttcctacctg ggcatgacac tcgaaacact cagccggatc
     5641 aggaaaaaaa ttacccggcc ctgattttct atttcataat tgcaaaagaa aaaagtaagg
     5701 ggatacacct aatttttgat ttcgtccggt catggctgaa aacaggtttc tcttagtgtt
     5761 gacttgagtg aaacagaatt cacctggcta tattatattt tactaagtgc ttctacatat
     5821 ttctttgaaa atcatccgcc gaaacagcaa tgggcctgag gaaaatcagg tgtattattg
     5881 gttggcgggt ggaaattgca gagcgtcctt tatttataat aagggataat gttgctctga
     5941 gaaattaatg ttttataatc tgcttgtgat acaggcgcat tcttcacaat attcggtcag
     6001 cgcttttata gggtaaggct attatgagaa gaaaaaaggt gaataagatt ttatcggtgt
     6061 tgctgttact gctgttcgtg gtagtaatct ccattttcta tattacctac agacaaagtg
     6121 aagacgtacg gtataacgca caaaccgttt cctttacagc cgaagtgata agcctttcga
     6181 cagccgttga actctcttca tatgactata ttctggcggt ccgggattat atcaacctcc
     6241 aagatcctgt gttaaaagta agaataaacg aaaaccaggc taagctgacc ggctatctta
     6301 accggctgac actgctggaa aaaaacgata tagaacaacc ttccactatt gattcactca
     6361 gtaaatacgt aacccggtta ttgcgcagcg ccgggttcat tgatagcaat ctttttgccc
     6421 ttccttccct tgaaagagat aaaatgaccg ctgctttaaa aacggaaatg cgttcacagc
     6481 tggggcagat caataaatac agcgaggtat taaaggaact ggaatacatg gagcgggaac
     6541 agcaacagaa cgcagaagaa aaatcgttgt tttggtcaaa actgatttta tatgcagtac
     6601 tgtttattgt acttgttctt actgctgtta ttgtattccg gatcaggact gaattttccc
     6661 gtcagtcggc gagtgaacaa aaatttatca ccttgctgga agcggctccg gatgccacca
     6721 ttatctcaaa tgagcagggc gtcattacat tggccaacag gcaggcagag aaactctttg
     6781 gctattcaat gaaggaactg gtggggttgg aggtggagct attgatacca aagcacatgc
     6841 gggaagcaca tgtgtcgcgc agaaaagggt ttactgacca gtcgcgggaa agagggatgg
     6901 gacacggtgt ggaaatgatt gcactacgga aggatggcat agaaattccg gtggaaatca
     6961 gtttgtcgcc aattgaaaca aaggaaggca aactcattgc cgccgctatc cgggatatca
     7021 cggcaaggaa aatggccgaa gcggaaatgc gccagttgta tacccagctt aatctcgcct
     7081 atgaagcact ctattttacg gatgaacacc tggtgattaa gacctggaat cacggggctg
     7141 aagtgctgta cggttacacc gcggaggaag ccattggccg ttattcggtg gaattattga
     7201 aaacggatct gacagcagaa gaatacaaag aagcacttac ggccatgacc aataacgatt
     7261 actgggcagg agaggtcagg cgtattaata aaagcgggga gctggtacat gtgcattcat
     7321 cactttcggc tatccggaac gaagcgaatg aagtgacagg ctatctctcg gtcagttttg
     7381 atatttcagc tgaaatgaag ctccgtgagg aagtccggaa gctcgcaaac ctgacggagc
     7441 aaatgtccga agcggtcatg tcgagaaagg ctggctccag cgaaatactt acctggaatg
     7501 cgggtgcgga acggctattc ggtttttcga aagaggaagc agtgggaaaa tcagccagag
     7561 aactgggtat tttacgcctg ggccctgaag tatttcagga aattgaaaat gccctgatct
     7621 caacagggga gtggaaggga gaaaaatgtt ttttcaggaa agatggttct tcctttacgg
     7681 gggctgtttc ttccaatgcc atacaaaatg accagcatga ccgatcttat gttttcttgg
     7741 ttaaggatat atctaccgag aaactgctgg cagctgcttt aaagaaaaat aatgaggaac
     7801 tcgaggaaaa ggtacaggta cgcaccctgg aaataggaca gagcgaaaaa cagtaccgat
     7861 atctttttga aaacaacccc atgcctatgt gggtatatga cctggatgat ttcaggtttc
     7921 tggatataaa tgaaatggcc atccataaat atggatatag ccgggaggag ttcctgaaaa
     7981 tgaccctgat ggatatccgg cccgaatcgg ataaggaacg tttcctccat ttcgaccatg
     8041 cctggaaaat gaattcacag gataccaacc ggggcgtttg gaagcattgc aaaaagaacg
     8101 gggagattat tgaagtggaa gtgattgccc attctatttt attccagaat aaaaaagcca
     8161 aactggtctt gctgaatgat gttactaacc gccttcaaac cgaacggaaa ctggtggcca
     8221 gtgagaaaaa attcagggcc ctgatagaaa acagcaatga tatcattgcc ttactggatg
     8281 cggatatgaa gatcacgtac cggagtccgt cggctatccg gattaccggc tggaccaatg
     8341 aggaaatcaa gaaaatgaat ttcctggatg aaattgtaca cccggatgac cgcgagaggc
     8401 tgaatgagtt gattcgcagt gtgctgttga gtccgggagt acctgtagtg gtttattaca
     8461 ggagccttca taataacggg aaatatattg atttggaagg ggtgctgatc aacctgatgc
     8521 accgggatta tatccgcgct atggtgttta atctccggga tgttaccgaa caaaaaaagg
     8581 cagaacagtt gttggtggca cgggagaaac gtttccgctc cctgatcgaa aacagttttg
     8641 atataattct cctgctggat gaaaatttta aagtggctta ccggagtccg tcggcagtac
     8701 ggatttcagg gcgctcggac gcggaagttt tgggaacgga tcccagggca ttaatacatg
     8761 gcgatgatct accgatgatt aaccagggta ttaaacgact catgctggcc cccggaagtt
     8821 ctgaacatgc tttgtttcgt tacccccata ccgatggtac atacctctgg atggagggct
     8881 acgctaccaa cctgttgcag gatgaaaacg taagatcaat tgtatttaat taccgggatg
     8941 tgacggccag ggttgaggca gaagaaagac tggtcaaccg ggagaagcat ttccgggccc
     9001 tgatcgaaaa cagtatggat gtgattgtgg tattggacga aaagttcaaa gtgatttacc
     9061 gaagtccatc tgctacaaaa attaccgggg tttcagatga agcaaccatc ggaaatgaca
     9121 gtcgcaccaa catacatcct gatgatctgg ggtcgctcaa tatcctgctc agagaattaa
     9181 ttaatttgcc gggaaaatca ttaaatattc tttttcggta tcggcgtgca gatggacgat
     9241 atatctgggt agaaggtagt gccaccaacc ttttggctga ggataatatt aaagggctgg
     9301 tattaaacct gcgggatgtg acagatcgta tcctgactga ggagaaagtc agggacagtg
     9361 aagagcgata ccgggccact cttgataata tgatggaagg ggtacagatt atcggctttg
     9421 actggaggta caaatatgtt aataaggccg ctgctgccca ggcgcatact acaaaagacc
     9481 agctgatcgg ggcgggcatg acagaaaaat ttccgggtat tgaaaatacc gaactatatc
     9541 agctgatcag ccgttgtttt accgaaagag tgccttttca aatggaaaac cggtttgtcc
     9601 tgcctgacgg ggaagaaata tggacacaac tgagtataca gccggtgccg gaaggggtgt
     9661 ttatactcag tgtggacatt actgaaaaaa taattgctgc tgcttcttta aaagaggaaa
     9721 agcataaact ggaatccatt gcggcttctt ctcccgggtt gatttattcc ttccagctaa
     9781 aacccgatgg atccatgagt tttccgtatg ccagtagtgc gatggaagat atgttcggtt
     9841 atggacacga actgatcaat caggatgtaa gcgcgcttct atcccatacc gttaaagagg
     9901 ataaggaagt gtttatagaa cgtttaaccc ggtctgcctt acaactgacc ccctggaacc
     9961 tggaattccg gtttcagcat ccggaaaaag gattgatctg gattgaagga aattcgattc
    10021 ctaccaaaga gccggatggg acgattacct ggcatggagt gattatggat gtaaccgaac
    10081 ggaaaaaaat tcaggataaa atcatagaac aggccgctca actgcgtacc ctgagtgata
    10141 acctgccagg tgtgatgatc tatcaaatgg tgggtaaatc attttacgaa cgccggttta
    10201 cttatgtaag taatgaagtg acccgcctta ccggcaaaac accggaagaa gttatcgcag
    10261 atccgtacct gctgtacaac tgtattttac cggaagatat acccctggtt tccctggcgg
    10321 aagaaaaatc gtttgagcaa cggacggtgt ttaatgaaga agtacgtttc cggactttca
    10381 acggagaaat taaatggctg aatataatat cgactccacg aaaaacagat caaggcgatt
    10441 ttgtctggga tggtttccat gtggatatta ctgagcgaaa aagtgcagag aatgtgatcc
    10501 gggaaagtga agaacgatac cgtacgctgg tcgaacaggc ttttgacggc atactatttt
    10561 atgacatcaa tggacaaatc ttagaatgta ataacagtgg tgccaacacg atgggatata
    10621 gccgggagga aatgctgcag atgaaccttg ccgatctttt ttacccggag gacctggaaa
    10681 tccggccgtt gaatatccag gatgtttcga gtggaatgca gattttcgat ttcagaacca
    10741 tgaaaaagaa agatggaaca ccggtggaag tggaatgggt gacccgttta cttccggatg
    10801 gacggatgct gggtgtgggc cgtgatatta cccatcagct gagggccctg tctcagcaaa
    10861 atctgctatc cgctattgtt aattcctcag atgacgccat tatcagtacc aatctgaata
    10921 gtgttatcag cagctggaat cttggggcag aaaagatata tggctatacc gctggggaaa
    10981 tgaaaggaaa tacggttaac gtacttattc cgccagatac attaaatgag gaagctgaaa
    11041 tactggcaaa aataatgaac ggggatatgg ttattcatta tgaaaccata catgtcagaa
    11101 aggatgggac aaagatattt gtttctatca ccacctcccc gttactggat agtaaagggg
    11161 ccgtgacggg tagttccaaa attatcaggg atatcacccg tcagaaggaa gatgcgaacc
    11221 ggatctcaca aaccaatgaa cgttatgaag ccgtagctaa agctaccagt gatgcgatct
    11281 gggatttcga ttatgaaacc aatcaaacct ttattgctgg taccggttac ctggatcttt
    11341 ttggctatcc gctggtcaat gtattcagtg aaccccggtt ttgggaagac cggattcatc
    11401 cggaggatta tgataaagta atggaagcga tggagcaggc caaaagggaa accactgtca
    11461 gtcaatccag cctggaatac cgtttccgtc gtgccgatgg cagttgggca tatctgtatg
    11521 atcgtttctt tattataaga gacaaagagg gcaaccccct gcgtttgctc ggggccaaac
    11581 aggatattac taaacgcaaa gaagccgaac aggagctgat tcagattgca gccgagaagc
    11641 aactgctcta tgaacgactg tcagtgatcc tcaataccct gccggccagt gtggccctgc
    11701 tggatccgaa gggagtgatg gtggaagtga atgatgcctg gaaaaacttt gcagatgtaa
    11761 atggattttc cggggttaat tatggtgtcg gtacgagtta tttcactgtt tccactcaat
    11821 cattcggaga taatgaacaa attggcaaag atatttcggc gggtgtacag gaagtgctga
    11881 atggcactct ttcgcaattc gaatatgaat attcctggca cctgaccaaa ctgaaccgct
    11941 ggttcaggat tgtcgttacg ccattgaaag gccgtgagtt caatggggtc gtagtgatgc
    12001 acatggatat ctcagaaata aggcggcttg aactggagcg gatcgaaagt aagatcgaag
    12061 aacagaagaa agtaacagaa gccatgttga aagggcagga gaaagaacgg aatgcgatcg
    12121 gaatcgagtt gcatgataat gtaaaccaga tactggtagg cactaaagta ctgctctccg
    12181 tggttcgtga ttttccggag aaaaccagtg aactgatccc ctcctgtatt gataatctca
    12241 gcctggccgt acaggaaaac cggaaaattg cccatgaact ggtgacgcct aatttatcgg
    12301 atgaaaacct gctgaaacag atcacccgcc tggctgatac tatgcttaaa aatgccggca
    12361 ttcaaaccta tatcaattcc gaaagcctga atgaacagtt actgagtgat gagatgaagc
    12421 tggcactgta ccgggtggcg caggaacaat gcaccaatat tattaaatac gcagaagcgg
    12481 aacaggttat catttccctg gcaaccaaga aggataattt ctttatgcgg atcgcggatg
    12541 acggtcatgg tacggatccc cagaaagtga cccatggcat cgggctgaaa aacatttcca
    12601 gccggctcgg ggtgttcagc ggcacgatca gtattgacac cgccccggga cagggatttg
    12661 ccctggagat tgaaattccg ttgacaccgg taaaaactcc agatttgccg gactgattct
    12721 cgtgccgtta agttaattat ctacttttta gacttgtttg gggtcgtaaa tggattaact
    12781 ttacctgcta aacaggcttg ttgtgaatag agttaattgt acccattctt tccgtttgat
    12841 cgtatgggcg cttgtattat ttttgcatca ttatacggct tccggtcaat tgtgtaccgg
    12901 tagtttggga gacccggttg tacatattga ttttggttct ccttccaatc cctccaccgg
    12961 ctttacagct cccggatata cttattccaa ttcttcctgt cccaatgatg gattttatac
    13021 tataacagga tatagtaccg gatgttttgg tacggcctgg catacggtgg ctgcggatca
    13081 tactggtggc ggcaattata tgctggtgaa tgcctcttat cagccaggcg atttttttgt
    13141 caagaccgtc agtaatcttt gtcccaatac aacgtacgaa ttttctgcct ggatcatgaa
    13201 tgtgctgata tccccaacgg gtatcaaacc ggatataacc ttccggattg aaacaccatc
    13261 gggtacatta ctgaatcagt atagcaccgg gggaatcagt gtgacttcat caccccgttg
    13321 ggaacagtac ggtttttatt ttactacaac agccggaaac acggatgtgg tgatccggat
    13381 gaccaataat gcgcccggag gcgggggaaa tgatctggcc ctggatgata ttatgttccg
    13441 gccctgtggt ccggtggtca cgtctgtcat acagggagtg agtttaccgg taaatgtttg
    13501 tgtatatgaa cagcagtctt ataccctggc cggttcactg tcaccgggtt ttgtcaatcc
    13561 ggtttatcaa tggcagcaat cgcttgattc cggccgaacc tggaccgata tacctggcgc
    13621 tgccagtgct acttataccc gcccaccctc cggagagggc cggtttcgct accggctatc
    13681 agtagccgaa agtggaaatg cggggattgc ttcctgccgg atcgcctcag agcaactgga
    13741 ggtaaatatt catccacgcc cgcaggttag tgcaggacct gataaaattc gtgttgccgg
    13801 ttatagtgat acccgcctta ccggtatggt atcgggcgat aatgttcagt atagctggat
    13861 cccacctgat tatttaagtt cggataccgc attgcaaccg gtggtaacag cggttaccga
    13921 tatgcagtac ctgcttaccg ccgtttcagg ggtgggttgt actaatgaag atgcggtact
    13981 ggtaaaagtg gtggccggcg tttttgtccc ttccgccttt acgcccaata acgatgggaa
    14041 aaatgatcgc tggaccatcc catttctgga tccctcctgg ggagcaagag taatggtgtt
    14101 tgaccgggcc ggacagctgg tttatcaggc caggggcgtg tcggttgact gggatggtaa
    14161 ttttaaagga atgccgctgg atgccggcag ttttgtttac tttatccagt ttgcagatgg
    14221 taagcctgat ctgaaaggaa ctctcaccct cattcgataa tactcctccc gatttgaata
    14281 caaatacagg cgtcttaccc gatcacctgt ctgctttccg gtgacggctg ttaaatggct
    14341 gttcacactt gtttattctt ccatataacc tggagccacc ggattatttt taccctgttt
    14401 aatagcaatt ctattagttc atgttatacg gatagaaatc cgggcattaa aaattgtgaa
    14461 ctgcacaggc agtttggtat gcaatgagcc tccctggttt tctaaccggg gatctttttt
    14521 ttacacttat caatgatccg gtaccagata agaaccggtg gatggcggga gacaatccgg
    14581 tcagaacggg aagaaagggg tgaatatttt agcagggtaa ttatccaata ttcactaaaa
    14641 aatcaacacc aaatgaaaat gaaattcatg gcgctgtgcc tgatggccgg cctgttcgta
    14701 ctcccttcct gtaagaaaga caaagaaacc actcctacag accctcccgt ggtggttaac
    14761 cccacactga gggctatcag tcctgctgcg ggacctaaaa acacattagt aaccattagt
    14821 ggtaccaact tcggaaccaa cagctccgtt ttgaaagtgt actttaataa tgtacaggcc
    14881 agtattcaat cggtgaatga taccctgatt accgcactcg tgccggtgaa tgcgggaacc
    14941 ggtactgtaa aattggagaa aaacagtact tcagtcaatg gacctgcttt tgagtattac
    15001 gtaaccaacc gggtactcac ttatgcgggc agcagccagg gctttgccga tggcaatctg
    15061 gctgcggcca ggtttaacat tgtgagcggc gtggtaaaag acgcctccgg taacttgttt
    15121 gtttgtgacc gggacaatca ccggatccgt aagatcactc cacaaggggt agtatccact
    15181 tttgccggag gtacaatggg ctttgcggat ggtaccggtt cggcggcccg cttcaaccag
    15241 ccctatagca ttaccattga tggcgcgggt aacctgtatg taggagaccg tatcaatcat
    15301 tctatccgca aaataactac ggctggcgtg gtaaccaccc tggcaggtaa tggaacggcg
    15361 ggtgatgcaa caggtaccgg tagtgccgcc cggtttaatg agccgattgg tatcgtttcg
    15421 gatcaggctg gtacattata tgtggccgat tatatcaatg cgaaaatcag aaaagtaacg
    15481 cctgcgggtg tggtcaccac ctatcagaat atcctggcat tcggcctggc aatggatgcc
    15541 gccggtaatc tgtatggtac cggttattat aatcatttcg tgtacaaata cagtccaaca
    15601 ggcgcatata gtgtagtggc cggtcaaaac ggggccgccg gtaatttaga tggcacggga
    15661 acgggcgccc agtttaattt tccgtcaggc ataaccgttg atgccaatgg caacctgtat
    15721 gtatgtgatg ccatgaatca ccggatccgc aaaatcacgg ctgcgggtgt ggttaccacc
    15781 ctggccggaa atgacccggg cgtgatggac ggcattggca gcggcgcttc ttttgataca
    15841 ccgttgggta tttgcagcga ctttgccaat aacgcggtat ttattgccga ctttaataac
    15901 agccggatcc gcaaactgat tattgattaa aacgtaaaat atgaacaaaa cagaatggtg
    15961 gcccaaagag ggcaaactca aaaaagggtt gatcatactg ggcatcgtaa ttgttgccgc
    16021 gttaataatg accctgatag aagaaaaagg ggtaaccttt accaagcgca tgccatttgt
    16081 aattgccctt tcttgtctgg cagtctggtt ttaccagccg gctaaaaagg aaaaagcata
    16141 ggccggcttt cagcaagcgc cggttgaacg tagacaagcc gttctccttt tccgataacc
    16201 actacccaaa ccgtattccc gcttttgcgg gggtacggtt ttttagttga taaccgcggt
    16261 agttgggctg ataactgtat ggttttggtt gaagtctgcc tgttatacac attctggtca
    16321 ttaatcagga aaaacagatt ctgtggtaat cgggtacttc cctattttta atggcatacc
    16381 acacctaagt tatccactat gaaaagtata tcttttcttt ttttatttgc ggctttttta
    16441 gcaatacagc catcggcggc acaaacctgt aattgtccgg agtactcgac gttgaaagaa
    16501 aaatgggcgt cctcggaaga tgatattgcc ggatatatca atcagttgcg aacctctcca
    16561 ggtaaactat gcaaagcgaa agcggatgaa tggatggctg ttgatttcat ggaaaacggg
    16621 aagtatgaca gttcagatct gtttttcaga aaggcagaag aaaaataccg ccagacaggg
    16681 tgtaacgata gtgtactggt gagtttgtat aaaaactggg cccagcttta ttatacaaat
    16741 gcggattttg gcaaagcaca ggaatatagt tttaagttat taaaggcggc tgaattatcg
    16801 ggtaatccgc atgaaatggc agtctgttat acgatgattg cgcagttgtt caatcagacc
    16861 ggtcaggctg acaaaggagt ggtctttaca cgtaaagcca tacccctggc ggataaggtg
    16921 actgatcctg ttaaaaagtc cgacctgttt tataaaattg ccaaacgata cctgtggcat
    16981 ttccaggata ccaaaatcag cagcagcctg gatagttccg agctgtttac tatcaggcaa
    17041 ctggaagtcg cccgttccat caatcgtaaa agtgcattag cggccggatt tagcaacctg
    17101 caagggatcg catgggaacg gggtgattta cggaaagccc tggtgttgct ggacagctct
    17161 tttacataca cggataagga cgaagacgat gtggtgggta ctaattattt tgacaaagcg
    17221 gatatctatg ttgccttaaa agactatccc aaagccgctc ttatggccga ttcggcatta
    17281 ttctaccgat taagggatgc taatccggtt tatacggcag aagtgtacga actgatttcc
    17341 cggatcaacc tggaatccgg gaattataaa ggagcgtatg aattcagaga gaaaggaaga
    17401 gccatcactg acagcgtcag gaatattgag aaaattaaag aagtatccga actggagaag
    17461 aaatacagcc aggagaaaaa tgaaaataag ataaaagacc tcgccagaca gaaacaggtc
    17521 tatctcttac tggcgattgc cggcttactg ggcctggccg tacttggttt ttttattcgt
    17581 cagcaatcgt tgagaaataa gcagaagatt ctcgaaacag agcaacgcct gaacagggcc
    17641 cggatgaatc cgcatttctt ttttaatgca ctgacctcgc ttcagcttca tgccctcgag
    17701 gaaaatgacg ggaagacact ggccagtaat atctccaagt tctcccatat aatgcgcgaa
    17761 acactggaga gcacgtataa agaatatgta accatagccc aggaaaagga tttcctggaa
    17821 gagtacatgg aactccagtc gatccggata cccgatcgtt tccggttctc tgttacttac
    17881 ggaacgacca ttgaaccgga ggatacaatt attccttcca tgattctgca gccctttgtg
    17941 gaaaacagta tcgaacatgg ttttgccggc attgattatg ccggacaggt ggatattcat
    18001 ttcgaccagg cacccggtga actgctggtc cggatttcgg acaacgggaa aggattgctg
    18061 aacgggccca aagagaataa tgaacatatc tccagggcca gtcagattat taaggatcgt
    18121 atttacctgc tgaatataaa gctccggaca aaagcctctt tttccatcga caataatgcc
    18181 catgaaaaag gggtaaccgt tttgatccgg ttacccctgt tatataaaga tcaggttcgg
    18241 taaatctatt tcagtttatc acagcctatt tcttttttac ccccgtaact gttgaacttt
    18301 attttgctgt cataatttcc gttaccggca taatcctgcc ggaatgtaaa ctcctggtaa
    18361 tagcacttgc catccggact tttgatggta catactgcgc tgaggtatcg atacatgatc
    18421 acgctggtga ggctgttctt ttctatattc caggaagagg aagtgatgat ggctttcaga
    18481 aatttatcat tccatccctc attattcgcg gccgctacca tttgttgttc gatagaagca
    18541 ttgctcatgc cggctttcgg taatttacgt ccggccatca gttcatcatg taatttagtg
    18601 gcctgtgttt taatcgccgt ggttccttca ttcagatcaa gtttcacatt gcttttgata
    18661 ttgttgataa tactggttgg aaaatgcaac tcacagctga tagtatggct gatatcgccg
    18721 ctacgaacca ggtcatcaaa gaagtaggag taggtccagt tttcatctgc caggtagggg
    18781 gcataatttg cagccagcca tttggcatca ggcagcaggg caaactgcag gtacgattta
    18841 ttctgtatgc ttaacggcaa caggatgttg gacaggtccc cgccccatac cgcggtgccc
    18901 ccatctatcc ggataatgat ccgcaattgt tcatttcctt tcatcagctg atttacatta
    18961 ttcccaaggt atacggtacc aaagatggct tccccggact tatactcatt tttaatggaa
    19021 gacgtcatct ctttttcctt gccaattacc aatggctggc tgctccatac aatctgattc
    19081 acgtgttctt tatgaaaatc gctggtgtaa aatttggccg cttcggcatc tgcattgccg
    19141 agtagttttt tgacctctgc cagtttttgc gtaaactcag ggtggttggg cgaaaggatc
    19201 aggactgctt cacattcgta ttttatatct tccagcagtt tcattttttc ctgcggatta
    19261 gcaatgtttt tactgcttgt cagtgcctgt accacattcc actttagccg gtcacttcgt
    19321 ttcaggtatt cgttataatc agccagtacc gcatcggtaa gggcgggata acttttggga
    19381 tcaaccgtgc cggctgcagc tcttttttct ttgtattcgg acagattaag cgccattacc
    19441 cggtcgtaat atactttgcc ggttacaccg ggttgtatat cgcggccgct ggaataaaca
    19501 ccgaccattt tctgatacat cgatttaaaa taatttcttt ctgcttcttc ctttgccttg
    19561 gcggaattct tatcaccgct ctctttggcc gctttatctt tccagatttt taattcggcc
    19621 tccattgccg ttgtattgta ggaaggatcc ttctccttta cctgctgaag catccgttct
    19681 gcattactga cggaggaact gatgccagta cccgccttca gtttttcaat atgactccag
    19741 aagtttttaa gcggcatttt agccggaccg gtatagccgg gatcctgggc aaggagtgaa
    19801 atggaaagga cgaggcataa taaagagcac agggcttttt tcataattga cagattttaa
    19861 ggataaagaa aggacctgga cctgtaatta tactgccgga tcgatggctg cacccggcag
    19921 gtcgatagtt gcaccaaaag atgtgataac tgccggttgc ccacattgca ggcggttttc
    19981 aggataaata gtttttgtgg tactggactt tgttggcata tttggagagc atagtccgct
    20041 tttctcagtc ccggacaatt gttcaaactc attgcaatga aagtattact gatcgacgat
    20101 gaagccaacc agcgcaacgc ggtaaagcag ttgttgcgtt ctttctgtcc gctggtacaa
    20161 accattgagg aagcgtccgg ggttaaaagc ggactgcagg ccattcgtga ttttcaacct
    20221 gatctggtat tcctggatgt ggaaatgggg gatgggacgg gttttgacct gatgcgggaa
    20281 atcagaaacc cggacttcca ggtggtattt atcacggccc ataataaata tgcgattacg
    20341 gcctttcagt acagtgcgat tgattacctg ctaaagccgg ttgatcccgc cgaattaaaa
    20401 agagccgtag accgggcggc cgttcatgtg aagaattcca gtttgcagga acagctccag
    20461 gtgctgatgc agcagctttc cgggattcat aaccaggacc ggaagattgt gctcaaggat
    20521 attgacaata cgtattttat caaagtgtcg gatgtcttgt attgtgaggc agccggctct
    20581 tatacccgtt tttattttac caatcagcag ccgatcctgg tatcccgtaa tctgaaagaa
    20641 tacgaagacg tgctggaagg ccttggtttt ctgcgtacgc accattcttt cctggtcaac
    20701 ccggaaaaaa ttaagttatt tgataaaacc gatgggggga tgctgatcct cgaaggaggt
    20761 ctgtccatcc cggtctccca aaggaaaaaa gattttgtaa tgcaggtcct ggaaaatcgt
    20821 tgagtgagtc ggttacggac ataaaaaaat cctccccgtt gacagggagg actttctgtt
    20881 attttattca gggattaacc tttgctgggc gctttttcaa tagctccttt ccctttcagg
    20941 taagcttcgt atgcttcagg cgtcagtact tctccggtaa tttttactga tttggcctga
    21001 tctattccgg caagtttaat aaacacatct ttgttgatcg gtccaactgc ggcagaatta
    21061 taatccactt tcagtttcac cgtggcaccg ggctgaatcg gttcaacgat tttttcaggc
    21121 atggtgcaac cgcaactggc ccaggtgttt tccaccacca caggtttgtc gctgatattc
    21181 ttgatctcaa aataatagga gacagggaca ttctgtttga ctttaccgaa atcatgattt
    21241 tcggtattca ctttgatcag ttcttcaggt ttcgtttggg cagaaacagc tacggtaaaa
    21301 agaaaggctg ttgcaaggaa taaaatcttc ttcatggttt gttattttaa aaggtttgtt
    21361 tttttaagaa atcaacagac gcattggccg gcgccggtcc atcaggggct ttccagacaa
    21421 agccttttat tcttatttgc ttattctggc tgctgttgtt gtaaaaaacc gtgatgtatt
    21481 tatcaaacat gccttcactg gccgcattga atccaacttt gattttcgta ctggctccgg
    21541 gcgcaatcgg atcacgactc cactcggggg tggtacaacc acaactggcc tggatattat
    21601 ccagcttaat cggggttttg cccttattta ctatttcaaa aaagtgataa accggctttc
    21661 cctggggaat tttggagaaa tcgtactcca tttccttgaa taaaaggtca gcattttcag
    21721 tcgtggcagc ctgtggggcc gtggagccat ggttatgccc gtcgttttca ttatgaaccg
    21781 gttgctgggc ttgtaaggcc agcagggagg tcatgcaaaa aagcagcagg gtgattttct
    21841 tcatactgaa cggtttagcg ctgtcaaatt taggggaaag atgccagatg tcatagcggg
    21901 gttgcacggg ttatccgtta ttttttaaca ggggatttgt acgcggtcat tctccctatt
    21961 tttgttgtat ggaaaatagc caatcggaac tcctgaccga tcccgggaaa ctgtcctatg
    22021 accgtttccg ggaagaggta ctcaatgact accggatagc ctgtatcagc agggaaacca
    22081 gtttgttagg gcgaaaggaa gtattgggtg gcaaggccaa atttggcatt ttcggggatg
    22141 gaaaggaact ggcccaggtc gccatggcca agttttttca gcccggtgat ttccgcagcg
    22201 gctactaccg cgatcagact tttatgtttg ccatcgggct gaccacggcc gaacaagtat
    22261 ttgcccagct ctatgccgac cctgatccca gccgggaacc tttcagttcc ggccgccaga
    22321 tgaattgtca cttttccacc cggaatgtca atgaacaggg cgaatggatg gaactggcca
    22381 ccaccaaaaa cgtagcgagt gatatggcgc ccactgcggc tcaaatgccc cggtcagtag
    22441 ggttggctta tgcatcaagg gccttccgga atgtggaaca actcaaagcc ttcagggggt
    22501 tatccgataa tgggaatgag gtctgcttca ccacgatcgg ggatgccagt accagtgaag
    22561 gtcatttctg ggaaaccatg aatgccgcag cggtattaca ggtgccaatg gcggtttttg
    22621 tatgggatga cgggtacgga atatcagtgc ctaagaaatt tcagacggta aaggcgtcta
    22681 tctctgatgc gcttgaaggg atgagaaaaa aagaaggcac taacgggctg aatatttata
    22741 aagtaaaagg atgggattat gccggaatgt gtgaagcatt tgaagaaggg atccgcctgg
    22801 cccgtgaaac ccatacgcct gttctcttcc atgtagaaga aattacacag ccgcaaggac
    22861 attcaacttc cggcagccac gaacgttata aaagcccgga gcggctggat tgggaacggg
    22921 aatgggatgg tatccgtaaa atgaaggaat ggctgatcgg aaatgaactg gccagtgaag
    22981 aagaactgga tgaaattgcc gaaaaagcca aggagcatgt gcgggaaagt aaggccagtg
    23041 cctgggcgaa atttgtaacg ccgattaaaa aacaggtagc cagggccact gacctggttc
    23101 acgccatggc gaatgcttta ccggaaaaaa gtgaggaatt gcataaagtg gcacgcgagc
    23161 tttctgccaa ccgggagccc cttcggcgtg atgtgcagaa aggagtgcat gctgccctga
    23221 atattgccgg cgatcatgat gccgctttct ggacaaagga tttctacaag gagctacagg
    23281 aagaaaataa aaaatactat aacacccatc tttacaatga agggcccaaa agtgccctgc
    23341 atgtacagga agtacatgcc gtgattccgg cagatgctgc tttactcaac gggtttgaaa
    23401 tcctgaaccg gtattttgat gaattgtttt ccagtaatcc caaagtgctg gccttcggag
    23461 aggacctggg ttttatcggg gatgtaaatc agggtttcag cggtctgcaa caaaagcatg
    23521 gggccgaacg catttttgat accggtatcc gggaactgac tattatggga cagggggtag
    23581 gactcgcttt aagaggtctt cgcccgattg ccgagattca atacctcgat tacctgcttt
    23641 atggcctgca accgctcagt gatgatgtat ccaccctgca ttaccgcacg ggaggacatc
    23701 agagttgtcc gctgatcgtg cgtacccgtg gccaccggct cgaagggatc tggcacagcg
    23761 gttcacccat gggaatgatc atcaattcac tccgtggaat gtatgtctgt gtaccccgca
    23821 atatgaccca ggcggccggt atgtataata ccttattgcg cagcaatgac cccggactgg
    23881 tgatcgaatg tctcaatggc tatcgcttaa aagaaaaact gccttccaac cttatggaat
    23941 ttaccgttcc acttggtgta ccggaagtgc tgaaagaagg atctgatatt accattgtgt
    24001 cttatggttc cactttgcgg attattcatg atgccgcgca tatgctcgaa gcggaaggtg
    24061 tcagttgtga aattattgat gtacaaaccc tcctcccgtt tgatattcat caccgcatcc
    24121 tggattcatt gaagaaaacc aaccggattg tctttgtgga tgaagatgta cccggtggtg
    24181 ctgccgccta tatgttcaat aaagtaatgg aagaacaggg cggctataaa tttctcgacg
    24241 tggctcccag aaccatcacc gccaaagccc atcgtccggc ttatggcagc gacggggatt
    24301 atttcagtaa gccgaatgcg gaggagatac agggggtggt gagggagatg atgagagagt
    24361 aaggcaagtc gggagtcggg agtcgggagt caacagcccc tccttaggag ctgttcggtg
    24421 ttagctgtta ggtgttagga acagcccgta ctaagaagct gtcgggtgtc aggtgtcggg
    24481 tgtcaggaaa cagccctcac gagagagttg tctgatgtca gttgtcagat gtcaggaaac
    24541 agccctttct tagagaggtt ggatttataa atgagtattc tgctttcttt catccggagc
    24601 gaaatcatcg tgtcatcctg agtagacgca taaggagatt ttcagggcag aagaaaaatg
    24661 atctgcgtcg tatcgaagga tggactaagt caacagccca tcctgagaac atatacctca
    24721 aggcacgtca gtcgtacttc gtacctcgta cttataacag ttgttctttg taaatcacat
    24781 gttctttctt ttacattatt tactatgaaa cttactctct accaaatcga tgcatttaca
    24841 gataatttgt ttggaggaaa tcctgcagcc gtcatcccct tgaagaaatg gctggatacg
    24901 gaactcatgc aaaagatcgc attggaaaat aacctcagtg agacggtctt ctttgttcct
    24961 tccaaaatga atggcgtgga ttatgatatc cgctggttta ccccgacttt ggagatcaat
    25021 ctatgtggac atgctacgct ggcttcttct tatgtgattt ttgacatact gaaatcgaat
    25081 aaacggaaaa tcgttttcca ttcacagagt ggcatgctga cggtgaagaa gcagaaggga
    25141 aaaatgtgga tggactttcc ttcctggaaa cccgaacgca cagccgatta tccggaggca
    25201 ttgggccaga tactgggtgg ggcagaagta gtgggcgtgt acaagcaccg tgactggctg
    25261 gtggaattgc aaaatgaaga agccgtgctg aagtgtgagc cggattttac gctgatgaaa
    25321 aagcatatgg acaaactcat tatcacggca cccggaaagg agtttgattt tgtctcccgt
    25381 ttttttgcac cggccgccgg agtctccgaa gatccggtaa cgggttcggc acattctcaa
    25441 ctgattcctt tctggtcgga gaaactgggt aaacataaaa tgacggcccg gcaattatcg
    25501 gcccgcggag gtacactgta ttgcgaacaa ctcagcaaag agcgggtact catgggcggt
    25561 acctgtgtgt actacatgaa gggaacgatc acccttcctt aatccaggaa tttctttttt
    25621 aatgcagggg tggggatcat acaggattcc tgccggccaa accaacggta ccggttacgc
    25681 gcgatccaac ggtaaacccc gtcgcggata aagcggggaa taaggatgta aacatagaat
    25741 atggaccagg ggaaacccag ccgtttcatc atcaggaggg caccggttga atgggtatag
    25801 attttgtcgc cctctgacag aacaaatgaa ttcagcgaat cagcggaaag accgtgtttt
    25861 gccaggatgg cctggcctgt tttgccttgt aacggggcaa aacggaaccg gtctttctta
    25921 tcccgtttaa tgataaactg cacactgctg ttacacagat tacaaacacc atcaaacagg
    25981 accagggagg ttgattcagc cattttacaa aggtaatcat ccgtggtttc cggaatatgt
    26041 tgtatttttt gggagtgaac cggtatcgat tatactttat tttgctggcc ttcttttatc
    26101 tgccaggtct tcagttgtcg gcacaaagca ggcaacagga ttcattgtat gtttttgacc
    26161 tgataaataa agcggatgtg ttttataatg gcggtcagta tgattccgct ttattgtact
    26221 gccgccaggc cgaagactgg agcgttcgta atcaatacaa aaccggtcag gcttttgcct
    26281 tgattaaaac cggggaaatc tatattgaca aagacgaact ggataaggca gcaaaatcgg
    26341 cagaagccgt aaataaaatc ggattacagc agcatgattc actggttacc gcaattggct
    26401 ggttacaaca ggggcaggta caactgtaca gtaatcagtt tgatgccgct ataaaattat
    26461 atgagaaatg cctgcagtat tacctgaaac aacacccgac ccgttattca ggacttgcgt
    26521 ataacgacct gggctattgc tggggcagaa agggtgacct gagccggcag gctgagtatc
    26581 tgtttcattc agtccgggta tacgaaaact attttccgga agaggccggc gaactgggtg
    26641 ctgcctataa taatatttca acggtctatt acggactcaa tgatatgaac aaagcgatcg
    26701 agtatgcgaa gatcgcgatg aagtaccggg agatcagcgg tgatgccggc ttgctttccg
    26761 tcagttgctg taatatcagc cagtattata tttccatcag taaggaggaa tctgaaaaat
    26821 acctgaaact ttgtgagcaa tatgccgtac aaagccgcga cgaaagacgc atgatccatt
    26881 catatgtgac ggcctataac ttttattaca gcaataaccg ttttgccgaa gcctaccaat
    26941 atgaactgaa gtcaattgcc ggcctggaaa agtcgggaaa aaatccggcg atgttgtccc
    27001 gccgttatat tggcgctgcg atctgtgcaa ataaactccg gatggattcc acgctgatat
    27061 tggggtattt ccggaaagcc aaaggaatgc tggatttact taaagaccgg atcaattacc
    27121 gggactattt ctggcagctg agtgattatt accggaatgc
//
LOCUS       DAOPMW010000011        26268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1638_length_26268_cov_8.196315, whole genome shotgun sequence.
ACCESSION   DAOPMW010000011 DAOPMW010000000
VERSION     DAOPMW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26268
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1638_length_26268_cov_8.196315"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLU11_01205"
     CDS             complement(<1..318)
                     /locus_tag="PLU11_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPN57750.1"
                     /translation="MCGITGFCDFSRKLTRDHLYAANEALHHRGPDSGDIALFDASEA
                     NIGFGHRRLSIMDVTSNGSQPMYSDDKSVVIILNGEIYNFKEIREDLLQKGYKFHSDS
                     DTEV"
     gene            complement(528..2213)
                     /locus_tag="PLU11_01210"
     CDS             complement(528..2213)
                     /locus_tag="PLU11_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57751.1"
                     /translation="MNKKIRVGLLIDDFGMTAWEFRMLERIVTGDFSEVVLVVKRKQS
                     PVVSRSFGDKLKENLAHGFFKVYSKIEPRLYKTETNAFATTDTKSLLGNIPLLEVDCI
                     EKKFSDFIKEDDLKQILTYEVDVFLRLGFRILRGGILKAAKMGVWSYHHGDNRHFRGG
                     PAGHWEVLKREKTVGSILQILTEDLDGGEVLYRSWSSVDVSATRTINSMFWKTALFVP
                     RMLKELHRLGENAFMEKIRLENEPFQFYSGKNYTIPGNTEFLRLIIPFFMNRVSHKFW
                     KLFNYHQWVLLYSFTKNNQPASTAYRYKRLLPPADRYWADPCVIQEGGRYFLFFEEVI
                     FQEVEQGHIAVAEINEKGFVSPPVRILERPYHLSYPFVFRYEGRYYMIPESEANATIE
                     LYEAVNFPFEWKFKMNLMEGVRAVDTTLLFREGKIWMFTNIRDIEGASYSEELFLFSS
                     DQLLTTDWKPHPGNPVVSDARSSRPAGRIFMHYGRYYRPSQDCSIRYGYATVFNEIMV
                     LNSEQYCEKQVSAITPEWANDLYATHTFSSEGGLSAIDAIIKRRKRGSKFFGR"
     gene            complement(2265..3356)
                     /locus_tag="PLU11_01215"
     CDS             complement(2265..3356)
                     /locus_tag="PLU11_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN57752.1"
                     /translation="MRILFFIDSFPAGGKERRLVELMKGLHAQPGYEFELVIMTRDVH
                     YKEVFDLCKQVHFVVRKTKKDFSVFGQFHRICKDFKPDIAHCWDSMTAVYLVPLSFVH
                     RFKLVNGMVADTPAVTGFRNTNWLRGKLTFPFSKAIVGNSQAGLNAYGAPARKRHCIY
                     NGFSFTRSENLVESKVMRQRLAIETPLVVGMVASFSRFKDYPVYYKAAEQLLSERKDI
                     TFLAVGDFTDSDASLAGVSPELRSYFRFLGRRSDAESLINCMNVCVLATFTEGISNSI
                     MEYMALGKPVVATDGGGTKEIVEDGKTGYLVQASQPAELADKIRILLNDPGKRVEMGK
                     TGQEKVKAQFLLTTMVERYIGLYNKLISG"
     gene            complement(3363..4484)
                     /locus_tag="PLU11_01220"
     CDS             complement(3363..4484)
                     /locus_tag="PLU11_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN57753.1"
                     /translation="MKFCFWGDIADAILGKTIGGGEIQIAQLARALARQGHEVVIIDF
                     EAEESFSTEEGIKVKSVPGWNKGIPGLKILIHQIPVLYRMLKAEKADYYYVRMRSYFN
                     ITSYLAARKNKAKFIVALAHDLDVAGFRDRFRYEYKSRLSLKSFFTLYLPNDLVFDYT
                     IRRADFITLQHKGQRLLSRRFRGKQQVFENIFDLNKVKDVVKQPGDFYVMAGSLTIMK
                     GIGNLLELVKRMDKSVNLVVIGEPRGKMAEAVYNELKKFPNVVLKGRLRHAETINYIA
                     SAKALVSTSNFEGFPNVFLEAWANGVPVMSLQVNPGNVIKEAGLGEFVNGDYEALAAC
                     MLANSTAAIQPAHLVNYINEHHSIEKAADKFLRFIGQPL"
     gene            complement(4557..5471)
                     /locus_tag="PLU11_01225"
     CDS             complement(4557..5471)
                     /locus_tag="PLU11_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN57754.1"
                     /translation="MTGNLPLVSIVMATYNRAGTIERAVNSVLSQNYPNLELIIVDDG
                     SKDNTLEILSQYTNPAIRIFKLETNKGVTAAKNAGLNQIRGEWFTILDSDDEMLPEAI
                     MTMIRIPLELDPEITAVTCNCLDTTTRRFSGIGPGKDGYVTVDELMTFRGEFWGITKT
                     QLLGNDRFNEKLRGFESTLWYKIDDRANRYYCHQALRVYHTEGQDRIMKRKYDFEKDV
                     VLYENLITEMEFLYKQKKYDPVDFNYLCRSGLMIMQLAGKKQIAGAYFGLLKGVNQGW
                     RTRMMMASRTAAALELWLVNLKKKIKKQ"
     gene            complement(5475..6650)
                     /locus_tag="PLU11_01230"
     CDS             complement(5475..6650)
                     /locus_tag="PLU11_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN57755.1"
                     /translation="MTIIIFGDLFSFPEGHAATNRIFTYAKGLKENGVHVAVICFSND
                     YLEQPDGVAEGIPYYNAFNRISRSPSFFARRWHKMKKYFRTYQLIKKINRQDKVMAIN
                     RWSDIPFTQFYIWLLSRFLGIKIITECNEHPLRYHQQGSWRRWIGRMRFRMDAFFADG
                     ILCISDYLVRFHQERGIHPRKLFLLPSTVDPTRFRQVGERPLGDFYIGYFGSLTFRRD
                     DIGVLLDAFSIVHQQGKTARLVLGGFCTDEERLKIRKRISELDLAANVLLVEYLSRNE
                     ILRYIGHADILVMVRARDLESDASYPSKLTEFLASGRPVLSVNVGEVGEYIKDGISAY
                     LVSPGDSKAMAEKLIYMIDHYSEALLVGQKGRELTDTIFNYNFQAKRLIEYIQKLNM"
     gene            complement(6665..7909)
                     /locus_tag="PLU11_01235"
     CDS             complement(6665..7909)
                     /locus_tag="PLU11_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN57756.1"
                     /translation="MNRQIQNILSLMYPFFPFLAWIAHFAGDKPVTFYFNILALPLAI
                     YFLVSSKRKLPAYLICFMLFVFYHLASSFINHTIPDNQNPAFFILYDYNVFAFAMFVV
                     IENTDFDVAFIEKMNRNIFYIIVISLLVSILQMQNPNFFFNPRVLMDNPEEFGEVEIR
                     NASIYSWYTANSGGITFPILISFALNYFEAQKSRFMFIVLAGILVSFLTKARYVMISA
                     ILVFSQLFISKGKSFTKLISLAFIFAAGIALFVFIADQVGYDLQKVISSRILEEDSDM
                     ESAKARVVSYEVFMKVFPQHPWLGVGPATGKDVVELLGGGIPLIHVGYLSYLYFYGLV
                     GCLFLFLSLFFLLRDGWRVGKKWGFWGSFYGLITFCVANATFVYFNFAEMGIVLCVIY
                     QRFYKTYEPEESELEDTPATTS"
     gene            complement(7915..8853)
                     /locus_tag="PLU11_01240"
     CDS             complement(7915..8853)
                     /locus_tag="PLU11_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57757.1"
                     /translation="MHPALSKYLSKLFIQQWVIGVGKCSPGDILESRVFDPEISWLDV
                     GTADRFFADPFLVKAADGQYRILFEDYTFSTGYGHIAELEVTDRLSLKKSKKILDTGS
                     HLSYPFIFRENGRIYIFPEAAQSGKLTCYEYHAETSSMEMVKVILEQPLLDSTIIKKD
                     GRYWLFCTRLGQGEDRQLHIYSSDQLLGDYKPHPMNPVKEDINGSRPAGALIAYKGEW
                     FRPAQDSRYTYGGGMVINKIVRLDEQEFAEEFYMRISPGIHSGRKAGIRGMHTLNFSD
                     ELLVTDGTRWRFAPIVKLRQLMMKFSHNNKQVKEKG"
     gene            complement(8886..10010)
                     /gene="rffA"
                     /locus_tag="PLU11_01245"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8886..10010)
                     /gene="rffA"
                     /locus_tag="PLU11_01245"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR02379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HPN57758.1"
                     /translation="MIPFNKPYLTGKEMHYMYQAVYSGKISGNGLFTQKCQAYFEKQS
                     GFKKALLTTSCTDALEMSAMLAAIEPGDEVIVPSYTFVSTALAFVRQGAKIIFADSSP
                     VNPNIDADKIEELISPRTRAIVPVHYAGIACDMDKIMALAAKYNLLVIEDAAQAIDSF
                     YKSRPLGSIGHLAAFSFHETKNINSGEGGLLAINDEKLVGRAEIIWEKGTNRSDFSRG
                     VVSKYEWVDTGSSFLPSEINAAYLFAQIEHLEDIQNRRKEIWEQYNKGLQGWASANGI
                     GLPQIPDYATNNAHMFYVVCKDGAQRNRLVEHLKSGEIWAVSHYLSLHRSPYYKGSDR
                     GAELVNSDHYTDCLLRLPLFYELSNEETARIISRICSFAG"
     gene            complement(10052..10741)
                     /locus_tag="PLU11_01250"
     CDS             complement(10052..10741)
                     /locus_tag="PLU11_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPN57759.1"
                     /translation="MKIAIISDIHGNFPALQAVLKDINTEGVDQVYCLGDMVGYYCMI
                     DEVIDTLREREIPSLMGNHDYALCFSNGVIERSKTCTHILGRQLREIRPDNREWLQRR
                     PAGLEFKLAAESYFCVHGGLEDPVDEYINKVDEDYFTRHAFVHDVLISGHTHQLRNEK
                     AGKFTYLNPGSVGQPRDGNPEAAYLLITETAKWHKRVPYEVDRVADAMKEKGYEPYIY
                     EILYRGVKIGG"
     gene            complement(10738..11688)
                     /locus_tag="PLU11_01255"
     CDS             complement(10738..11688)
                     /locus_tag="PLU11_01255"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPN57760.1"
                     /translation="MYNILVTGVGSIIGYGIIDGLRKSTIPVSITGVDIYDDAYGAVI
                     ADHFEKGVRADAPEYIDFINQVVDQYKIDLIIPGIEQDLYAMWEHRDKISTKVVFGNE
                     LCMQMAKDKLFTFEYLYEKNIRLIPTLHHCTFSECRDKFGLPFLLKPILSSASKGIEL
                     IRTEEEFDFYSRRAGGKCIYQKIVGSMEEEYTANVFGDGNGGLFDVIILKRKLSGEGA
                     TSKAVYTDNPLLLEYIKVLCTLLKPDGPMNIQLRTEAGVPYLLEINSRVSSSCSIRTS
                     MGYNEPEMCLKYYLTGEKIVPAPRIPGRAIRYIADYFIAT"
     gene            complement(11717..13174)
                     /locus_tag="PLU11_01260"
     CDS             complement(11717..13174)
                     /locus_tag="PLU11_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPN57761.1"
                     /translation="MNSKKTVIVGSGLSALMMARMIKKYRDPMADIVILEKEEQVGGQ
                     YGSINYGQYGYFDVGMHIIYETCIPEIDNLFEDLLTEEEWIILDNNYKDIAGIYVNGR
                     LQTDTPYVDLRDSSTEKWREYVAELFQVIRSGKECQLPEEPNSYDLLVNHFGKRITDE
                     IFVPILEKLYFKHPSELAEIANKFTTINRLALFDSATMLDLMKSDEIRARICYPDQLS
                     MPQYRQNTQKGFYPREYGMFRVINRFRKQLEMEGVTFQTSCSITNLQLDQQTITSIEV
                     KGNNGNVESLPVKELFWTAGLPGLAATLGIPVKDLTFEKKKTEDVYVNLLFDKKPSMG
                     ELYYFYPFDTEFRSFRVTSYTNYCPSAAGERGYPVCVEFWAREEDSKTEENLILQACE
                     ELKTFGVIDDSYQLLFGKVERKMAGFPLPSVTNISTMNTINERISKAGITNLTATGVL
                     TGKNVFFIKDVLIDTYNKVISNRPMQSISNFKNAI"
     gene            complement(13171..14472)
                     /locus_tag="PLU11_01265"
     CDS             complement(13171..14472)
                     /locus_tag="PLU11_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN57762.1"
                     /translation="MNILNLATTDENAAGVACRNANDMFLKAGHNSLLLVKESVSGYP
                     GVLALKKRKYGTYIHTLPGRIENRLSLLYRKLRPFDRDDKYSFHNLDEGKKHISGKDI
                     LDAVPFKPDVILIFWVSNFINMETVSEMAALTEAKIMWMMTDNAPLTGGCHYPWDCKG
                     FHTDCNNCPAILTKGSKSIAEKNLAIKKKFIPGNAEIMSSSESDYQRAKSSALFKGKV
                     IHKLLAPIDENKFKPGDKTAARAFFGIPEGKKVIFYGANAFSDPRKGGTNFLNALNIL
                     KETLRQPDSKCNPSDVLLLLAGKEKPVHFLDIGFEVKEAGYLNENELITAYNAADFLV
                     SPSLEDSGPMMVNQSIVCGTPMLAFDIGVAMDLIENGVTGYKARLFDDEDLANGLKYL
                     LSLSEDELQTMSVNCRNFALQHMTYAEYTRRLTAIIQKQPL"
     gene            complement(14491..15168)
                     /locus_tag="PLU11_01270"
     CDS             complement(14491..15168)
                     /locus_tag="PLU11_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HPN57763.1"
                     /translation="MKNNKKQLVIIGSSSNARLAKFYFDTDSDREVVAFAVNRDYIKE
                     ATFEGLPVVALEDLSVHYPPDSYDAFVAVGYNQMNKVREKLYNEAKNIGYLLINYISS
                     RCTFLSQFPVGDNCFILEDNTIQPYVKIGNNVVLWSGNHIGHDVTIGDHCYITSQVVI
                     SGFVDVKPYCFLGVNATIRDAVTIAEATLVGAGAVIMKSTEEKGVYLPPRSTIFNKKS
                     DEVVISS"
     gene            complement(15158..15862)
                     /locus_tag="PLU11_01275"
     CDS             complement(15158..15862)
                     /locus_tag="PLU11_01275"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPN57764.1"
                     /translation="MKKVAISQSNYIPWKGYFDAINEVDEFMLFDDMQYTKRDWRNRN
                     KIKTKEGAKWLTVPVEVKGKYFQKINETLISDPKWGQDHWNSILHNYNKAPFFKDYKD
                     IFEPLYLASQEDHLSLVNYNFIKAICEILGITTKISWSSAFELKEDRNERLVDLCMKT
                     GATDYYSGPAAKAYMDVSLFEKENIQVHYYDYSGYPVYNQLHGEFDHAVSILDLLFCE
                     GPESKKYMKTFVENEK"
     gene            complement(15971..16615)
                     /locus_tag="PLU11_01280"
     CDS             complement(15971..16615)
                     /locus_tag="PLU11_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HPN57765.1"
                     /translation="MSIRHVGIIGNGVIGKQLEHLIAEGHKSGDLSFTYFDDRMFANN
                     EPSSYPLLEYTNAQFTNLEFYIGLGYKCTTLKRSVADRLFEGNFRFPAVIHYSACLHP
                     SVHVGNGVVIYPMCNLGFDVKVGYGSIINKSCTIAHDSVIGKCSFLAPSITLCGGVKV
                     GDNCFIGAGTIVADNVVIGNNVTVGIGSVITKDIADGLQVIGNPARVVSELNIR"
     gene            complement(16642..18009)
                     /locus_tag="PLU11_01285"
     CDS             complement(16642..18009)
                     /locus_tag="PLU11_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57766.1"
                     /translation="MLSSLTKPVSFIKSLVNKGHDRSINAKKNILASFIIKGGSIAIS
                     LVSVPLTIHWVHPDQYGIWLTLSSLVSWFSFFDIGFGNGLRNKFAEAKAKGEHELARI
                     YVSTTYAILSIISGFILILFICVNPFLDWAIILNAPRTMSAELGLLALIVFGFFSIDF
                     VLQLIATILNADQQPAKASLFSFLGGLLSLIIIFILTQTTKGNLIYLGLSFVGTQLVV
                     LLVATIWLFSGKYKPYAPSLKFVHFKYARGLMTLGLKFFIIQIAFIVVYETTIIIIAQ
                     LDQSQVTPYNIAFKYFSVIPMIFGIIMVPFWSAYTEAYVKKEMDWIRSTMKKLITIWG
                     FFTIMGIVMLLVSDKVYELWVGPEIKVPFTLSLAIVTYIIVNAWCTIYSVFLNGVGKL
                     KLQFYSGIIGALINIPLAIYMGKKFGVTGVVLSSLVLALGNALWSPVQYKKIMNNTAT
                     GIWNQ"
     gene            complement(18166..18717)
                     /locus_tag="PLU11_01290"
     CDS             complement(18166..18717)
                     /locus_tag="PLU11_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPN57767.1"
                     /translation="MNESKNWYAVYTRPRCEKKVAEQLSRKKIECYCPINKVVRQWSD
                     RKKVVFEPLFATYVFVNAAETELAQLKQLDGVVNLVYWLQRPAVIPEREIEAIRHFLQ
                     DNINVTLQKTTINISDRVRIISGPLMAHEGNVLSVKSRTVKLSLPSLGYLMIAEVQSA
                     SVEVLLPRGLTMPGLEYPLLASK"
     gene            complement(18921..21272)
                     /locus_tag="PLU11_01295"
     CDS             complement(18921..21272)
                     /locus_tag="PLU11_01295"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPN57768.1"
                     /translation="MHTFPKKGQPDQEKNVLSQAWFKYFPYWPLFLILLVIAGAGAWF
                     YVRYKTSPYYIATASILIKDEKKGQDEAKMLESLNQLSAKKIIENEIEVIRSRELLSQ
                     VARKLHLYAPIYEKHKVLPLSGYTSSPVRIQANNPDSLVTNVGDVYFTMDSSRTHVVI
                     KGEAYPLNKWVSTPYGNLRFVPNNITGYLPAQPFYFNLVDPKKIATYLQGSLTAVSVS
                     KLSSVISLKITDENAKKAEDILNALIVSYNEAALNDKNTLAANTLAFVQERLKFVEKD
                     LDSIEQKLQQYKANQGAIDIGSQGTLFLENVSAISQKLGDVNVQADVLDQVEKYVTSK
                     DKSGSIVPSTVGVNDPLLTKLVNDLYTNELEYDKLIRTTGPNNPMALTLKSEIDKMKP
                     SILENIRNQKTGIKANRDNLNSLNSRYSSLLSSIPKKERDLVEISRQQNIKTSVYNFL
                     LQKYEETAMSLQSSIADSRVVDSALSFGPINPGKKIYMIAGFLALILGIGLVTANEML
                     KRTILFRHEIEAFTSIPVVGEIVHDDSGEAIVIGPGKRTFIAEQFRNLRTSLPYLGIK
                     NNKKKILVTSTVSGEGKSFVVANLAVSLALAGKKVIVLEFDLSDPTLCDKLGFTNVNK
                     GIKDYLNGDAEPEDVIRRTSAHENLFIMPAGRPLPDNPSELIMSEKVPELLQYLSDIF
                     DYIVVDSAPVGLLSDAYVLSAYCDATLYVIRHKHTRKVSVQRLDANNKINELKNMAIV
                     FNGVKSRGFSKNGYGYGYGYGYIHNEKRDKVKKNKSTVSQERS"
     gene            complement(21287..22060)
                     /locus_tag="PLU11_01300"
     CDS             complement(21287..22060)
                     /locus_tag="PLU11_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPN57769.1"
                     /translation="MQLTFLRRVCVPVMVLVFLASCVDTRKATYFNGVNTGTINEKTP
                     VPESIIRPNDLLSITVSSLNPEATAVFNAPNNAINQPGGGASGYLVNADGTIQFPLLG
                     SIQTAGLTKEQLKDKIRKSIVDRQELKDPIVSIRYLNFRVTVLGEVRNPTVLTIPNEK
                     ISLLEAIGLAGDLTIYAKRDNVLIIREENGLKNIQRINLNSNELLTSPYYYLKSDDVV
                     YVEPNKSRVASANSNSQWVPIALSMLSFGVIVVDRLVNN"
     gene            23059..>26268
                     /locus_tag="PLU11_01305"
     CDS             23059..>26268
                     /locus_tag="PLU11_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57770.1"
                     /translation="MKRLLVAALITLTLPSFARVYYTSPSGNDNNAGSITAPWFTLQR
                     AWTSVAAGDTVYMRGGTYTYTVQPYLTGKNGTASNLIKLWAYPGESPVITRGTSFNKS
                     NGWHRGMVFLTGNYIHVKGIRFTGMYTDDNQVDAGLQTYNVNNCIFELLECDNNVEGM
                     IVENNSNNNLILNCDFHDNYSNYGGSNGGNSDGLGLTYMVGLNTVTTVRGCRAWNNGD
                     DGFDTFENSGYVLMDSCWAWHNGYFKGTNTAAGDGVGFKLGSDFLTTPAGVGIVKRRL
                     QRSLGFDNANAGAHINEADFSTELYNNVFFRNKITGLNFHYNNRVHYFRNNVSFGNTD
                     KDVEISGNSVRSNNSAGTAGNDGGWTTNASAADFMSTDTTGVRNTRQANGTLPNLNFL
                     KFSSSSDLIDAGLNIGLPFNGTAPDRGAYEFGVALPNQTPTANAGTDKTITLPTNSIT
                     QTGSGTDPDGSIAAYQWTKIAGPATFTIVSPTQAATVINNVVQGTYQFELRVTDNLGA
                     TARDTMILTVNAAANIAPTANAGANQTITLPVNTVTVTGSGTDTDGSIATYQWTKIAG
                     PATFTIVSPTQASTVINNLVQGTYQFELRVTDNLGAIGRDTMSVVVNATNTPPTANAG
                     ADQTITLPANTVTVTGSGTDPDGSIASYAWTKISGPVTFNIATPNQAASVINNLVQGI
                     YIFSLTVTDNAGATGIDYITITVNAAGNQSPAANAGADRTISLPVNSLTVTGTGTDPD
                     GSIAFWQWTKIAGPVTFNIVSPTQASTVINSLVQGVYRFELRVTDNLGAVARDTMTVT
                     VNAAPNQAPTANAGADQIINLPTNTVSVTGSGTDADGSIASYQWTKIAGPATFNIVSP
                     TQAQTTINALVQGSYQFELRVTDNLGAIDRDTMTITVNPLPNQPPVANAGADLTITLP
                     VNTVTLNGVATDADGSIITYQWTKISGPATFTIVSSSTPQTAVNGLVQGIYVFRLTVT
                     DNNGASATDDVTVNVNPAPNQAPVANAGADRTITLPTNSLTVTGTGTDTDGNIASYQW
                     TKISGPATFSITSPTQAQTLISNLIQGTYQFELRVTDNLG"
BASE COUNT         7279 a         6296 c         5389 g         7304 t
ORIGIN      
        1 tacttctgta tccgaatctg aatggaattt atagcccttc tgaagcaggt cttccctgat
       61 ctccttaaag ttgtagattt caccattgag aataatcacc acacttttat catcactata
      121 catgggctgg ctaccattac tggtaacatc catgatggaa agccgccggt gtccgaaccc
      181 gatatttgct tcgctggcat caaacaaggc aatatcccca ctatccggcc cgcgatgatg
      241 cagggcttca ttggccgcat agagatgatc cctggtcagt tttcttgaaa aatcacaaaa
      301 gccggttata ccgcacatag taaaggttta tgggtaaaaa aacaggtcgc ttatcgtaaa
      361 aagccgaaaa cagcgacttt gttaaaacgt aaaattttca ttttatttcg tttatgcctt
      421 aataatcagc ataaaaaaca agcggttatg acgtaatgag tatttttacc aactgaaatt
      481 taccacgtaa aattacgttt aagaatcagt tcttttagca gtaattatca acgtccaaaa
      541 aacttcgatc ccctttttct ccgttttatg attgcatcga tcgcagaaag ccctccttcg
      601 gatgaaaagg tatgggttgc gtacagatca tttgcccatt ccggggtgat ggcagatacc
      661 tgtttttcac agtattgttc actgtttaat accatgattt cattgaaaac agtggcataa
      721 ccatagcgga ttgagcaatc ctgtgaaggc cggtaatacc ggccatagtg cataaaaatc
      781 cggcctgcag gtcgggaaga gcgggcatcg gagacaaccg ggttgcccgg atgtggtttc
      841 cagtcggtgg tcagtaactg atcggatgaa aagagaaaca gttcttcact gtaggaagcg
      901 ccctcaatat cacggatatt ggtaaacatc catattttac cctccctgaa caaaagggtg
      961 gtatccaccg cccggactcc ttccatcagg ttcatcttaa acttccattc aaagggaaaa
     1021 ttcactgctt cataaagttc tattgtagca ttcgcttccg attcaggaat catatagtaa
     1081 cggccttcat acctgaatac gaatggatac gacaggtgat atggcctttc aagaattcgt
     1141 accggagggg atacaaatcc tttctcattt atttcggcca cagcgatatg tccctgttca
     1201 acttcctgga aaatcacttc ttcaaaaaag agaaaatacc gacctccctc ctgaattaca
     1261 caaggatccg cccaatagcg atcggctggc ggaagcaacc gcttataccg gtatgcggtg
     1321 gaggctggct gattattttt ggtaaaggag tataaaagca cccattgatg ataattaaaa
     1381 agcttccaga acttatgtga cacccggttc ataaagaatg ggataatcag gcgcaaaaac
     1441 tctgtgtttc cgggaatagt ataattttta ccactataaa actgaaaggg ttcattctcc
     1501 aggcgaatct tttccataaa agcgttttcg ccaaggcgat gcagttcttt gagcatccgg
     1561 ggaacaaaca atgccgtttt ccagaacatg ctgttgattg ttcttgtggc agaaacatcc
     1621 acagaagacc aggaacggta aagcacttcc cccccatcaa gatcctctgt cagaatctgt
     1681 aaaattgaac caaccgtttt ttcgcgtttc agaacttccc aatgaccagc agggcctccc
     1741 ctgaaatgcc ggttatcccc atgatgatag gaccatacac ccattttagc agctttcagg
     1801 atgccccctc gaaggatccg gaaacccagg cggagaaaca catccacttc gtaggtgagt
     1861 atctgcttca ggtcatcctc tttaataaaa tcagaaaatt tcttttcaat acaatcaacc
     1921 tcaagcaggg gtatatttcc gagtaacgat tttgtatcgg ttgttgcaaa ggcattggtt
     1981 tccgttttat ataaacgggg ctctatcttt gaatagactt taaaaaatcc atgcgctaaa
     2041 ttttccttca gcttatcgcc aaatgacctg gaaacaaccg gtgactgttt gcgttttacg
     2101 acaagcacca cctcactgaa atcaccggtc acgattcgtt ccagcatacg gaattcccag
     2161 gcggtcatcc cgaaatcatc aatcagtaat ccaacgcgga tttttttatt cataatgctc
     2221 agcgtttgta aaaaatcagc caaaaataga aactttggtt gctattaacc tgaaattaat
     2281 ttattgtaaa gtcctatata tcgctccacc atcgtggtca gcaggaattg tgcttttact
     2341 ttttcttgcc cggtcttacc catttcaact cttttcccgg gatcattcaa caggatccga
     2401 atcttgtcag ccagttccgc aggttgactg gcctgtacca ggtaacctgt cttcccatcc
     2461 tccacaattt ccttggttcc tcccccatcg gttgccacaa ccggtttgcc aagcgccatg
     2521 tattccatga ttgaattgga aatgccttcg gtaaaagtgg ccagtacaca tacattcata
     2581 cagttgatca gggattccgc atcggaacgc ctgcctaaaa aacggaaata cgatcttaat
     2641 tcaggtgaca cgccagccaa cgaggcatcg ctgtctgtaa agtcacctac tgccagaaaa
     2701 gtaatatcct ttctttctga aagcaactgt tcggctgctt tataataaac cgggtaatcc
     2761 ttaaaccgcg aaaaactcgc aaccataccc actacgagcg gtgtttcgat ggccagacgt
     2821 tgcctcataa cctttgactc caccaggttt tctgagcggg taaaactaaa tccattataa
     2881 atgcagtggc gctttcgtgc cggtgcgcca tacgcattca gtcctgcctg tgaattacca
     2941 acaatggctt tactgaatgg aaaagtcagt ttaccccgga gccagttcgt attacgaaaa
     3001 ccggtaacag ccggggtatc cgccaccatc ccgtttacca gcttaaaacg gtgaacaaac
     3061 gataatggaa ccaggtaaac agcggtcata ctgtcccagc aatgtgcgat atccggttta
     3121 aaatccttgc agattcgatg aaactgtcca aaaacagaaa agtctttttt ggttttgcgt
     3181 acaacaaaat gcacttgctt gcacaaatcg aatacctctt tataatgcac atcccgggtc
     3241 atgatgacca gttcaaactc atatccgggt tgtgcatgca atcctttcat gagttcaacc
     3301 agccgtcttt ctttgccacc ggcggggaat gagtcaataa aaaaaagtat ccgcataatt
     3361 ggttataatg gctggccgat aaatcgcagg aatttatcag ccgctttttc gattgaatga
     3421 tgttcgttta tataatttac cagatgtgcc ggctgaatag cggccgtact gttggccagc
     3481 atacaggcag ccagtgcctc ataatcaccg ttcacaaatt ctcccaggcc tgcttctttg
     3541 atgacattac ccggattcac ctgaagcgac atcaccggca caccgtttgc ccaggcttcc
     3601 agaaacacgt tgggaaaccc ttcaaaattg gaggtactga ccagcgcttt ggcactggcg
     3661 atataattaa tggtttccgc atggcgcaag cgtcctttaa gcactacatt cggaaatttt
     3721 ttcaactcat tgtataccgc ttctgccatt tttccccttg gctccccgat taccacaagg
     3781 ttgacacttt tatccatccg cttcacgagt tccagcaggt ttccaattcc tttcatgatg
     3841 gtgagtgatc ccgccattac ataaaaatcc cccggttgtt tcaccacatc tttcacctta
     3901 ttcaggtcaa aaatattttc gaatacttgt tgctttcccc tgaaccggcg tgaaagaagc
     3961 cgttgtcctt tatgttgaag agtaataaaa tccgcccgac gaatcgtata atcaaaaaca
     4021 agatcattcg gcaggtaaag tgtaaaaaaa cttttcagac tcaaccggct tttatattca
     4081 tagcggaacc ggtccctgaa cccggctaca tccaggtcat gcgccagggc cacaataaat
     4141 ttagctttgt tcttcctggc agcgagataa gaggtgatat tgaaatagga acgcatccgc
     4201 acataataat aatctgcttt ttctgctttc agcatccggt agagcacagg aatctggtgt
     4261 attaatatct tgagtccggg tatgcctttg ttccatccgg gtactgactt tacttttatc
     4321 ccttcttcgg ttgaaaaact ttcttctgct tcaaaatcaa taataaccac ttcatggcct
     4381 tgcctggcca gcgccctggc cagttgggca atctgtattt cccccccccc gatcgtttta
     4441 cccaggatag catcggcaat atcgccccag aaacaaaatt tcatcgttta tagtttaaaa
     4501 tcccggcaac cggattgaaa aaatccggaa cggaaacagt tttcagaaat attgatttat
     4561 tgttttttta ttttcttctt caggttcacc agccataatt ccaatgctgc ggctgtgcgg
     4621 ctggccatca tcatccgggt ccgccaaccc tggttcactc ctttcagcaa cccaaaatac
     4681 gctccggcaa tttgtttctt tccggccagt tgcataatca ttaacccgga ccggcacagg
     4741 taattaaaat caacaggatc gtatttcttt tgcttgtata aaaattccat ctcggtaatc
     4801 aggttttcat acaatacaac atccttttca aagtcatact ttctcttcat tatccggtcc
     4861 tgcccctcgg tatgatatac ccggagtgcc tggtgacaat aataacgatt ggcccggtca
     4921 tctattttat accacaatgt gctttcaaat cccctaagct tttcattgaa acgatcattt
     4981 ccaagaagtt gcgttttggt aatcccccag aattccccgc ggaaagtcat caattcatcg
     5041 actgtcacat acccgtcttt gcctgggcct atgccggaaa acctgcgggt ggttgtatcc
     5101 aggcaattac aggtaaccgc tgtaatttcc ggatcgagtt ccagcggaat ccggatcatt
     5161 gtcatgatgg cttctggcag catttcatcg tcagagtcca gtatggtaaa ccattctccc
     5221 cggatctgat tcaacccggc attctttgcc gcagtcaccc ccttattcgt ttccagtttg
     5281 aaaatccgga tagccggatt tgtatactgg ctgagtattt caagcgtgtt gtctttagag
     5341 ccatcatcca caataatcaa ttccaggttc gggtaattct gggacagcac gctattcacc
     5401 gctctttcga tggtgccggc cctgttatag gttgccatga caatactaac cagtggaaga
     5461 ttacctgtca tagatcacat atttagtttt tgtatatatt caatcagcct tttcgcctga
     5521 aaattataat tgaatatggt gtctgtcagc tcccggccct tttggcctac cagcagtgct
     5581 tcactgtaat gatcgatcat atagattagc ttttctgcca tggccttact gtcacccggg
     5641 gaaacaagat aagcgcttat accgtcctta atatattcac ccacttcacc aacattgaca
     5701 gaaagtaccg gtcgtccgct ggccaggaat tctgtaagtt tggaaggata ggatgcgtct
     5761 gattccagat cgcgcgccct taccattacc aggatatccg catggccgat gtaccggagt
     5821 atctcatttc ttgaaaggta ttcaaccagc aggacgttcg cagctaaatc cagttcacta
     5881 atccgtttcc tgattttcag tcgttcctca tcagtacaaa atccacccaa aaccagccgg
     5941 gccgttttac cctgctggtg aacgatggaa aaagcgtcca gtaatacacc gatatcatct
     6001 ctgcggaatg taagtgaacc aaaatacccg atatagaaat cccccagcgg cctttcaccc
     6061 acctgcctga atcgggtagg atccacagta ctgggtaaca ggaataattt gcgtggatgg
     6121 atcccccttt cctggtgaaa ccttaccagg taatcagaaa tacagagaat tccatccgcg
     6181 aaaaaagcat ccatccggaa tcgcattcgc ccgatccacc ggcgccagct accctgttga
     6241 tgataacgta aaggatgctc attgcattcg gtgattattt taattcccaa aaaccgggac
     6301 aggagccaga tataaaactg ggtaaagggg atatcactcc accggtttat cgccatcact
     6361 ttatcctgac ggttgatttt tttaatcagt tgataagtcc ggaaatattt tttcatttta
     6421 tgccagcgac gggcaaaaaa agacgggctc cggctgatgc ggttaaaagc attataataa
     6481 ggtattcctt cagccactcc gtccggttgt tcgaggtagt catttgagaa acagatcact
     6541 gctacgtgta caccattctc ctttaacccc ttggcatagg taaatatcct gttggtggcc
     6601 gcatggcctt caggaaaact gaaaaggtca ccaaaaataa tgatcgtcat tgcaggtttt
     6661 tttctcagga agttgttgca ggggtatcct ccaattcact ttcttccggt tcataggttt
     6721 tataaaaacg ctgataaatc acacacagga ctatacccat ctcagcgaaa ttaaaataca
     6781 cgaaggtggc attggccaca caaaaggtaa tcaggccata aaaactgccc cagaatcccc
     6841 attttttacc cacccgccag ccgtcacgta acagaaaaaa taaggatagg aagagaaaca
     6901 aacaacccac cagtccgtaa aaatagaggt aggacaggta gcctacgtgg atcaggggta
     6961 tccctcctcc aagtagttca accacatcct ttcctgtagc cggccccaca cccaaccatg
     7021 gatgctgcgg gaatactttc ataaaaactt cataggaaac cacccgcgct ttggccgatt
     7081 ccatatccga atcctcttcc aggatacgac tggagattac tttttgcaga tcatatccca
     7141 cctggtcggc aataaaaaca aaaagcgcaa tacctgcagc gaagatgaaa gcaagtgaaa
     7201 tcagtttagt gaatgatttg ccttttgaaa taaacaattg tgaaaaaaca aggatagcgg
     7261 agatcattac gtagcgggcc ttggtcagga aggagaccaa tataccggcc aaaacgataa
     7321 acatgaagcg gcttttctgt gcttcaaagt agttgagcgc aaaagagatc aggatcggga
     7381 atgtgatacc gcccgagttc gccgtgtacc aggagtaaat cgatgcattt ctgatttcca
     7441 cttcaccgaa ctcctcggga ttatccatca gcacacgtgg gttgaagaaa aaattcggat
     7501 tctgcatctg cagaatactt accagcagtg aaatgacaat tatgtaaaaa atattacggt
     7561 tcattttttc tataaacgcc acatcaaaat ccgtattctc aataaccaca aacatggcga
     7621 aggcgaatac gttataatca tatagaataa agaacgccgg gttctggtta tccgggatgg
     7681 tatgattgat aaaggaagaa gcgagatgat aaaacacaaa aagcataaag cagatcaggt
     7741 aagccggcag tttgcgtttt gaactgacca ggaagtaaat ggccagtggt aatgccagga
     7801 tattaaagta aaaggtcacc ggcttatctc cggcaaaatg ggctatccat gccaggaacg
     7861 gaaagaaggg gtacatcaat gacaatatgt tctgtatctg ccggttcata gtttctatcc
     7921 cttctctttt acctgtttat tgttatggct gaacttcatc atcagctgcc tgagttttac
     7981 tattggtgca aagcgccagc gggtaccatc agttaccagg agttcatccg aaaaatttaa
     8041 tgtatgcata ccccggattc cggctttccg gccactgtga atgcccgggc tgatccgcat
     8101 ataaaactcc tcggcgaatt cctgctcatc caaccgtact attttattaa tcaccattcc
     8161 tccgccataa gtatagcggc tgtcttgtgc cggacgaaac cactcaccct tataggctat
     8221 cagcgcgcca gccggccggc tgccgttgat atcctctttt accggattca tcgggtgggg
     8281 tttataatcg cccaggagtt gatccgaaga ataaatatgc aattgccggt cttctccctg
     8341 gccaagccgg gtacaaaaca accaataccg gccgtctttc tttatgatag tactatccag
     8401 taacggttgt tccagtatta ctttaaccat ctccattgaa ctggtttccg catgatactc
     8461 ataacaggtc agcttgccac tctgagcagc ttccggaaaa atataaatcc gtccattttc
     8521 cctgaaaata aacggatagg acaaatgact gccggtatcc agaatttttt tacttttctt
     8581 cagtgacagc cggtcggtaa cttccagttc agcaatatgt ccatagccgg tagagaatgt
     8641 atagtcttca aataaaatcc ggtattgtcc atctgcggct ttcactaaaa acgggtcagc
     8701 gaaaaatcga tcggctgttc caacatccag ccagctgatc tccggatcaa ataccctgct
     8761 ttcgagtata tcacccggac tgcattttcc cacgccgatc acccattgct ggataaacaa
     8821 cttggagaga tattttgaaa gagccgggtg catctgcgta tgattaaaat aaataggtgg
     8881 tccgtttatc ctgcaaaaga gcagatacgg ctgatgattc tggccgtttc ctcattactt
     8941 aattcataaa acaggggcaa tcgcagaagg caatcggtat agtggtcact gtttaccagt
     9001 tcagcaccac gatcggatcc tttataataa ggactgcggt gaaggctgag gtagtgggaa
     9061 acagcccata tttccccgct cttcaggtgt tccacgagcc ggttacgttg tgcgccatct
     9121 ttacatacca cataaaacat atgggcattg ttggtcgcat aatctggtat ttgagggagg
     9181 ccaatcccat tggcacttgc ccagccctgt aaccctttat tatactgttc ccatatttct
     9241 tttctcctgt tttgtatatc ctccaggtgc tcgatctgcg caaaaagata agcggcatta
     9301 atttctgaag gaaggaaaga ggatcctgta tccacccatt catatttgct aacgacaccg
     9361 cgcgaaaaat cgctgcggtt tgttcctttt tcccagataa tttctgcacg gcctaccagc
     9421 ttttcgtcat tgatagccag tagcccccct tcaccggagt taatattctt tgtttcatga
     9481 aatgagaaag ccgcaagatg cccgatagac ccaagggggc ggcttttata aaacgaatcg
     9541 atagcctggg ctgcatcttc aatgaccagc agattatact ttgcagccag ggccataatc
     9601 ttgtccatat cgcaggcaat acctgcataa tgtaccggca caatggcacg ggtccgggga
     9661 ctgatcagtt cttcaatctt gtccgcatca atattcggat tcaccggtga actgtctgca
     9721 aaaattatct ttgccccttg ccgtacaaaa gcaagagcgg tggatacaaa tgtgtaggat
     9781 ggaacaatga cttcatcacc cggctcaata gccgcaagca tggcactcat ctccaatgca
     9841 tcggtacagg aagtggtcag cagtgctttt ttaaaaccgg attgcttttc aaaataagcc
     9901 tggcatttct gggtaaacaa cccgtttccg gaaatttttc cggaatatac ggcctggtac
     9961 atgtaatgca tttctttacc ggtcagataa ggtttgttaa atggtatcat ctgctattta
    10021 tttcaattca tgaatagtag tacgctggtt ttcatccgcc aatttttaca ccgcggtata
    10081 aaatttcata tatatagggt tcatatcctt tttccttcat cgcgtccgcc acccggtcca
    10141 cttcgtaagg aaccctttta tgccactttg ctgtttcagt gatcagcaga taagcggctt
    10201 ccgggtttcc gtcacggggc tggcctaccg atccgggatt caggtaagtg aacttccccg
    10261 ctttctcgtt ccgtaactga tgggtatggc ctgaaatcag cacgtcatgc acaaaggcat
    10321 gacgggtgaa atagtcctca tcgaccttat taatatactc atctaccgga tcctccaatc
    10381 caccatgtac acaaaaataa gattcagcag ccagtttaaa ttctaacccg gcaggccgtc
    10441 tttgcagcca ctcgcggtta tctggtctga tttcccgtaa ctgtctgccc agaatatggg
    10501 tacaggtctt tgaccgttca atgactccat tactgaaaca gagtgcatag tcatggtttc
    10561 ccatgagtga gggaatttct ctttccctta atgtgtcgat cacttcatca atcatacagt
    10621 agtaaccgac catatcgccc aggcaataga cctggtccac cccttccgta ttgatatctt
    10681 ttaataccgc ctgtaaagcc ggaaagttac catggatatc agaaattatg gcaattttca
    10741 tgtggcaata aaataatctg cgatataacg gatcgcccgg cccggaatac ggggggctgg
    10801 tactatcttt tcaccggtca ggtaatactt caggcacatt tccggttcat tataacccat
    10861 agatgtacgg atagaacagg aactggaaac cctggagttt atttccaaca ggtaaggcac
    10921 gcctgcttct gtccgcagtt gtatattcat cggcccatcc ggctttaaaa gggtgcaaag
    10981 caccttaata tattcaagca gcaatggatt atctgtatag accgctttac tggttgcccc
    11041 ttctccggat agtttccttt tcaaaataat tacatcaaac aacccaccgt ttccgtctcc
    11101 aaagacattg gcggtgtatt cttcttccat agatcccact attttctggt aaatacattt
    11161 acctcctgca cgacgggaat aaaaatcaaa ctcctcctct gtccggatta attcaattcc
    11221 tttagaggcg gaggaaagta tgggtttcag taaaaaaggc aggccgaatt tatcccggca
    11281 ttcagaaaaa gtgcagtggt gcagggtagg tatcagccgg atattctttt cgtacaggta
    11341 ctcgaaagtg aataatttat cctttgccat ctgcatgcac aattcgtttc caaaaaccac
    11401 tttggtgctg atcttgtcgc gatgctccca cattgcataa aggtcctgtt caataccggg
    11461 tataatcagg tcaattttat actgatcaac aacctggtta ataaaatcta tatactccgg
    11521 tgcatccgcc ctgacccctt tttcaaaatg atcagcaatc actgcgccgt aggcatcatc
    11581 gtaaatatca accccggtaa tactgacagg gatagttgat tttcgtaatc catcaattat
    11641 tccatatccg attatagatc ctactcctgt gactaatata ttatacatat cctctctgct
    11701 gtttggcagc cggtgatcag atagcattct taaaattact gatcgattgc atcggccggt
    11761 tactgattac cttattatag gtatctatta aaacatcctt aataaagaac acattcttac
    11821 ctgtcagcac ccctgttgcg gtcagattag tgataccggc cttgctgatc cgttcattaa
    11881 tcgtattcat tgtgctgata ttggttacgg atggcaaggg gaatcccgcc attttccgct
    11941 ccactttccc aaacagcaat tggtagctgt cgtcgatcac tccaaaggtt tttagttcct
    12001 cacacgcttg taagataaga ttttcctcgg tcttggaatc ttcttctctt gcccaaaact
    12061 ccacacatac cgggtagccc ctttcacctg ctgctgaagg acaatagttt gtataactgg
    12121 tcacccgaaa agaccggaat tctgtatcaa atgggtagaa ataatataac tcacccatgg
    12181 atggcttttt gtcaaacaac aggttgacat atacatcttc cgtttttttc ttttcaaacg
    12241 tcaggtcctt aaccgggatc ccaagtgttg ccgcaagccc gggtagtccg gctgtccaga
    12301 atagctcttt tactggcaat gattcaacat tcccgttatt gcctttcacc tctatggaag
    12361 taatagtttg ctgatccagc tgcagattgg tgatagaaca ggatgtctgg aatgtcacgc
    12421 cttccatttc cagttgtttc cggaacctgt taataaccct gaacattcca tattcccggg
    12481 gataaaatcc tttttgcgta ttctgccggt attgtggcat gctgagctga tcaggatagc
    12541 agatgcgtgc cctgatttca tccgacttca tcagatcaag catggttgct gaatcaaata
    12601 atgccagccg gttaatggtc gtgaatttat tggcaatctc tgccagttct gaaggatgtt
    12661 taaagtatag tttttccagg atcggtacaa aaatctcatc ggtgatacgt ttaccgaagt
    12721 gattaaccag cagatcgtat gaattaggtt cttctggcag ttgacactct ttacctgacc
    12781 ggataacctg gaaaagttca gccacgtatt cccgccattt ttctgtggag ctgtcccgca
    12841 gatccacata gggggtatcg gtctgtaacc ggccgtttac atatatacct gcaatgtctt
    12901 tataattatt atccagaata atccactctt cttccgtgag gaggtcctca aaaagattat
    12961 caatttcagg aatacaggtt tcatagatta tatgcattcc tacatcaaaa tacccatact
    13021 ggccatagtt aatactacca tactgcccgc ccacctgttc ttctttttcc agaattacga
    13081 tatcagccat cgggtcccgg tattttttaa tcatacgagc catcatcagc gcagaaagtc
    13141 cgctgccaac aattacagtc tttttgctgt tcataagggt tgcttttgta ttatagcagt
    13201 cagtctgcgt gtatattcag catacgtcat atgctgtaac gcaaaatttc tgcaattcac
    13261 tgacattgtc tgaagttcgt cctcagacaa agacagcagg tatttgagtc cgttggccag
    13321 gtcttcatca tcaaataacc tggctttata gccggttacc ccattttcga tcagatccat
    13381 tgctactccg atatcaaaag caagcattgg agtaccacaa acgatactct gattgaccat
    13441 catggggccc gaatcttcca gtgaggggga tactaagaaa tcagccgcat tgtaggctgt
    13501 aattaattca ttttcattca gatatcccgc ttcttttact tcaaacccga tatccagaaa
    13561 atgaacaggc ttttctttgc cggccagcag gagcaataca tccgagggat tgcatttact
    13621 gtccggttga cggagtgttt ctttaaggat attgagtgca ttcaggaagt tggtaccccc
    13681 tttccgcgga tcactaaacg catttgcccc atagaatatt acttttttac cctccggaat
    13741 gccaaaaaaa gccctggcag cggttttgtc tccgggtttg aacttgtttt cgtcgatcgg
    13801 ggcaagcaat ttatggataa ctttcccttt aaacagcgct gagcttttag cccgctgata
    13861 atcagactcc gaactgctca taatttccgc attgcccggg ataaattttt tctttatagc
    13921 caggtttttt tccgcaattg atttggaccc ttttgtcagg atggccgggc aattattgca
    13981 atcagtatga aatcccttgc agtcccaggg gtaatggcag ccgcccgtaa gcggggcatt
    14041 atccgtcatc atccacatta ttttggcctc agtcaatgcg gccatttcac tgaccgtttc
    14101 catattgata aaattcgaga cccaaaagat cagaatcacg tcgggtttga aaggaaccgc
    14161 gtcaaggata tctttaccgg aaatatgttt ctttccttca tcaagattat gaaaagagta
    14221 cttatcgtct ctgtcaaacg gacgaagctt ccggtataac agggacaacc tgttttcaat
    14281 tctgccggga agtgtgtgga tgtaggttcc gtattttcgt ttcttcaatg ccaggactcc
    14341 ggggtaacca gaaactgatt cctttaccag taacagggaa ttatggcccg ctttaaggaa
    14401 catatcgttt gcattcctgc aggcaacgcc ggcggcattt tcatcagtgg tagctaaatt
    14461 gagaatattc atgggagatc agcgcaattt tcaggaggag ataaccacct catcactttt
    14521 cttattgaaa atcgtggagc ggggaggtaa atatacccct ttttcttcgg tgcttttcat
    14581 aatcacagca ccggcaccca ccagggttgc ttcggcgatg gtaactgcat cccgtatagt
    14641 agcattcacc cccagaaaac aatagggttt cacatccaca aaaccggaaa tcaccacctg
    14701 tgaagtaata tagcagtggt caccaatcgt aacatcatga ccgatatgat tgccgctcca
    14761 gagtaccaca ttattgccaa tcttcacata gggctgtatc gtgttgtcct ccaaaataaa
    14821 acaattatcc cctacaggaa actgcgacag aaaggtgcag cgtgagctga tatagttgat
    14881 caataggtaa cctatgtttt tggcctcgtt atatagcttt tccctgacct tattcatctg
    14941 gttataccct actgccacaa acgcgtcata cgagtctggc ggataatgaa cggacaggtc
    15001 ttccaatgcc accacgggaa ggccttcaaa tgtagcttct ttaatatagt ccctgtttac
    15061 tgcaaaagcc accacttcac gatccgaatc ggtgtcaaaa tagaatttcg caagtcttgc
    15121 attggatgag cttccaataa taacaagttg tttcttatta tttttcattt tcaacaaagg
    15181 ttttcatgta tttttttgat tcgggacctt cacaaaataa gagatccagg atactaaccg
    15241 catgatcaaa ctcaccatgc aactgattgt aaaccgggta tccgctgtaa tcataataat
    15301 gaacctggat attctccttt tcaaaaaggc ttacatccat ataggctttg gcggccggtc
    15361 cggagtagta atccgtggcc cctgttttca tacaaagatc caccagtcgt tcattccggt
    15421 cttctttcag ttcaaaagcg gaagaccagc tgatctttgt ggtgatcccc agtatttcac
    15481 atatggcttt tataaaatta taattaacca ggctgagatg atcttcctgg ctggccaggt
    15541 atagtggctc aaatatgtct ttatagtctt tgaaaaacgg agccttattg taattgtgca
    15601 aaattgaatt ccagtgatcc tgaccccatt tcggatcact gatcagggtt tcatttattt
    15661 tctgaaagta cttccctttt acttccaccg gaactgtcag ccatttggcc ccttcctttg
    15721 tctttatttt attccggttc cgccaatccc tcttggtata ctgcatgtca tcaaagagca
    15781 taaattcatc cacttcgttg atggcatcaa aatatccctt ccaggggata tagttagatt
    15841 gtgatatcgc gacttttttc atttttttta ttcaggatta aaggcttatt ccggttgcca
    15901 aagcaaaagc ggcaagccgg aggagcgaca aaatataaat ttttcccggg agtttatagt
    15961 aaatcggtaa ttacctgata ttaagttctg acactaccct ggccgggttg ccgatcacct
    16021 gcaatccatc tgcaatatcc tttgtgatca ccgaacctat tccaacggta acgttattcc
    16081 cgatcacgac attatctgcc acaatagtac cagctccgat gaaacaatta tcgcccactt
    16141 ttacaccccc gcacaaagta attgaagggg ccaggaaaga gcatttcccg atcactgaat
    16201 cgtgagcgat ggtacaggat ttattaataa tgcttccata cccgacttta acatcaaaac
    16261 caagattaca catcggatag atgacgaccc cgtttccgac atgaactgat ggatgcaggc
    16321 aggcggaata atgaatcact gcggggaagc ggaaattgcc ctcaaaaagc cggtcggcaa
    16381 ccgatctttt caacgttgta catttataac ccagtcctat ataaaattca agattggtaa
    16441 attgggcgtt ggtgtattcc aacagggggt aactggaagg ttcgttattg gcaaacattc
    16501 tatcatcaaa ataggtaaaa gaaaggtcac cgcttttgtg gccttcagca attaaatgct
    16561 ccagttgttt cccgataaca ccatttccaa taatgccgac atgcctgata ctcataccgt
    16621 ttcattttag aataagatag gttattgatt ccatatccct gtagcagtat tattcatgat
    16681 cttcttatac tggacgggcg accagagcgc gttacccaat gccagcacga gcgaggaaag
    16741 taccacaccg gtaaccccga attttttacc catgtaaata gcgagcggga tattgatcag
    16801 ggcaccaatg ataccgctgt aaaactgcag ttttaatttt cccacgccat tcaggaaaac
    16861 agaataaata gtacaccagg cattcacaat aatataggta acaatggcca gggacaaagt
    16921 aaacggaact ttgatttcag gtcccaccca cagctcatat actttatctg aaacgagcaa
    16981 catcacaata cccataatgg tgaaaaagcc ccagatggta atgagttttt tcatggtgga
    17041 acgaatccag tccatttcct ttttaacata cgcctcggta taggcactcc agaaaggaac
    17101 cataataatc ccaaaaatca tggggatgac actgaaatac ttaaaagcga tattgtaggg
    17161 agtcacctgt gactgatcga gctgggctat aataatgatg gtggtttcat acaccactat
    17221 aaacgcaatc tggataatga agaatttcaa tcccagggtc atcaggcctc ttgcgtactt
    17281 aaaatgtaca aatttcaggg aaggggcata gggcttgtat ttacccgaaa aaagccagat
    17341 agtagccacc aggagcacta ccagttgggt gccgacaaaa ctcagaccga ggtaaatcag
    17401 gttacccttt gttgtctggg tcagtataaa tataataatc aaagaaagca ggccccctaa
    17461 aaaactgaat aaggaagctt ttgcgggctg ctgatcggca ttcagaatgg tggcaataag
    17521 ctgaagtaca aagtcgattg aaaagaagcc aaatacaatc agtgccagca atccaagttc
    17581 agcactcatt gtcctggggg cattcaggat aatggcccag tcaagaaatg gatttacgca
    17641 tataaaaaga atcaggataa atccactgat aatactgaga atcgcgtagg tggtactcac
    17701 ataaatacgg gccagttcat gctcgccttt agccttggct tccgcaaatt tattacgtaa
    17761 gccattccca aatccgatat caaaaaaact gaaccagctg accagtgaac tgagcgtaag
    17821 ccagatgccg tactgatccg ggtggaccca atgaatcgtt aaaggaaccg ataccaggct
    17881 gatcgcaata cttcctcctt tgataataaa ggaagccagg atgttttttt tggcattgat
    17941 actccggtcg tgccctttat tgaccagact tttaataaag gaaaccggct tggttagact
    18001 gcttaacatt aagatttaca tagtttacag atcctgccag ccacaatagt cattgcagca
    18061 gtatctaatg atggtacagg atcgggcaac aaagattgcc gttttaattc acactttcta
    18121 attatctctc aaaaataacc aatagccgga agatgggccg gttgattatt tggaagccag
    18181 taacggatat tccagtccgg gcatggtcaa ccctcttggc aagagtactt caacactggc
    18241 cgattgcact tcagcaatca tgaggtatcc cagtgaagga agtgacaatt taacggtcct
    18301 gcttttcaca gaaaggacat tgccttcatg tgccataagg gggccactga tgatccggac
    18361 acggtccgat atgttgatcg tcgttttctg aagagtcaca ttaatattat cctgaagaaa
    18421 gtgccggatg gcctcaattt cgcgctcagg aatcaccgcc ggtctttgca accagtatac
    18481 aagattcacc acgccgtcca actgtttcag ttgggccagt tcagtttcgg cagcatttac
    18541 aaaaacatag gtggcaaaaa gcggttcaaa aaccactttt ttccggtcac tccattgcct
    18601 gaccacttta ttgatcgggc aatagcattc gattttcttt cgtgacagct gctctgccac
    18661 tttcttttca catctgggac gggtgtaaac agcgtaccaa tttttacttt cgttcataaa
    18721 accaggttta acaaaacact taaatcaata gaatttttaa ttctacgggt cattcacagc
    18781 attgatttct ctgccttaac tggtttttgc acggaacggg gtacggcttc gccttcatca
    18841 gtcacaatgg tgacgaaaac agcgccattt taaaatacat ccattctaca taacaacacc
    18901 tgtcggctaa tctatcctat ctaacttctt tcctgagaaa cggtcgattt attcttcttt
    18961 actttatctc ttttttcatt atgaatatag ccgtagccgt atccatatcc atatccattt
    19021 ttactgaatc ctcttgattt caccccattg aacacaatgg ccatattctt cagttcattg
    19081 atcttgttat tggcatccaa ccgctgcacc gacacttttc gggtatgttt atgccggatg
    19141 acatacaagg tcgcatcaca ataggccgat aacacatacg cgtctgataa aagaccaacc
    19201 ggggcagaat ccaccacgat ataatcaaat atatcggaaa ggtattgcag cagttcgggt
    19261 actttttcac tcatgatcag ctctgaagga ttgtccggaa gcggacgacc tgccggcatg
    19321 ataaacagat tttcgtgtgc ggacgtcctt ctgatcacat cttccggttc tgcatctcca
    19381 ttcaggtaat ctttaatccc tttgttcacg ttggtaaatc cgagtttatc gcacagggtc
    19441 ggatcactca ggtcaaattc cagcacgatc acctttttac ctgccagggc caggctaact
    19501 gccaggttcg ccaccacaaa gctttttcct tccccggaga ccgttgaagt caccagaatt
    19561 ttctttttat tgttcttgat cccaagatat ggcagggaag tacggagatt ccggaattgc
    19621 tctgcaataa aggttctttt accaggtccg ataacaatag cttcaccgga atcatcatga
    19681 acaatctctc caaccactgg aatggaggta aatgcctcta tttcatgcct gaacaggatg
    19741 gttcttttca acatttcgtt ggcagtaacc agtccgattc ccagaattaa tgccagaaaa
    19801 ccggcaatca tgtaaatttt cttgcccgga ttgatcgggc cgaaggacag cgcactgtcc
    19861 accacccggc tatccgcgat ggaagattgc agggacatgg cggtttcctc gtatttctga
    19921 aggaggaaat tatatacgct ggtcttaata ttttgctgac ggctgatctc cacgagatca
    19981 cgttccttct taggtatgct gctgagtaag gatgaataac ggctgttcag ggagttcagg
    20041 ttatcccgat tggctttaat acctgttttc tgattccgta tattctccag aatactgggt
    20101 ttcattttat caatctcact tttcagagtg agtgccatcg ggttattagg ccccgtggtt
    20161 ctgatcagct tgtcatactc cagttcgttg gtataaaggt cattcaccag tttagtgagc
    20221 aaggggtcat ttaccccaac tgtggaagga acaatactgc ccgatttatc tttggaagtg
    20281 acatattttt ccacttggtc gagtacatcc gcctgaacat tcacatcccc gagtttctga
    20341 ctgatggcac tgacgttttc aagaaaaagc gttccttgtg aaccgatatc aatcgccccc
    20401 tgatttgctt tatattgctg caatttttgc tcgatggagt caaggtcttt ttccacaaac
    20461 tttaaccgct cctgtacaaa ggccagggta ttggcggcca gggtattttt gtcattcagt
    20521 gccgcttcat tataagaaac gatcagtgca ttgaggatat cctccgcctt ttttgcgttt
    20581 tcgtctgtaa tttttaaact gattacagaa gacagtttgc tgactgaaac ggctgtcaga
    20641 cttccctgta ggtaagtggc aattttttta ggatccacca ggttaaaata aaagggctga
    20701 gccggtaaat accctgttat attattaggt acaaaacgta aattaccgta aggagtggaa
    20761 acccatttat tgagtggata cgcttcccct ttaatcacca catgggtcct ggaactatcc
    20821 atggtaaagt acacatctcc aacattcgtc accagcgaat cagggttatt ggcctgtatc
    20881 cgtaccgggg aagaagtata accggaaagg ggtaatactt tatgtttttc gtaaataggc
    20941 gcatagagat gaagtttcct ggccacctgg gaaagcaatt ccctggaccg gatcacttcg
    21001 atttcgtttt cgataatttt tttggcagat aactgattca gtgattccag cattttggcc
    21061 tcatcctgac ctttcttctc atccttgatc agaatggaag cggtagcgat gtaataaggg
    21121 ctggttttgt acctgacata gaaccaagcg cctgccccgg caataaccag tagaatcagg
    21181 aagaggggcc aataaggaaa atacttgaac caagcctggc tcaatacatt tttttcctgg
    21241 tctggctgac cttttttagg aaatgtgtgc atagttttct gggttcttaa ttattaacta
    21301 accggtctac aacgatcaca ccaaacgata acatactaag ggcgattggc acccattgtg
    21361 aattactgtt ggcagacgct acacgcgatt tattcggttc cacatagacc acgtcatcgg
    21421 atttcaggta atagtacggt gatgtcagca gttcatttga attcagattg atccgctgaa
    21481 tatttttaag cccgttttcc tcacggataa taaggacatt atctcttttt gcatagatgg
    21541 tcaggtctcc ggccaggcca atggcttcca gcagggagat tttttcattt ggaatggtca
    21601 gcacagtagg gttcctgact tctccaagaa ccgttaccct gaaattcaga tagcggatgg
    21661 aaacaatcgg atctttcagt tcctggcgat ccacaatcga tttacggatt ttatctttca
    21721 attgctcctt cgtcagtcca gcggtctgga tactacccag caaaggaaat tgaatcgtac
    21781 catcggcatt gaccaggtaa ccggaggcgc ctccacccgg ctgattgatg gcattattcg
    21841 gtgcattgaa tacagccgtt gcttccggat tcagactgga aacggtaatg ctcagtaagt
    21901 cattgggcct tatgattgat tccgggacag gtgttttttc attgattgta cccgtattca
    21961 ctccattgaa ataagtggct ttccgggtat ccacacagga agccagaaaa actaacacca
    22021 ttactggtac acaaacccgc cttaaaaaag ttaactgcat atgtttccat ttaagccttt
    22081 agggctaatt tattgtacta ccaatcagct taacttgaat cagtggtttt gcgaaggatg
    22141 aaggtacatg cggggcttta cgaaggatta aaggatacgg tcgtactgca agataatcag
    22201 cgatatacgg ccaattcgag tacaagatac aaatcataaa tgaaatcgtg tagtgtttac
    22261 gaaaaaaaaa tgtggttttc cctgaatttt tacctaggcc atagtaaaac tacgtataag
    22321 gtatttcctg atttcccggt aagtatttac tgctaattct gattctgaat gatttgtgca
    22381 cattttacta tagcgccaga ccgcggcacc tggaccaata aagccggatt ttttataaaa
    22441 tattgatccc tacagaccgt atacaggatt aagatttctc aaaaatcccg aatcccccgc
    22501 cgtgtttttt cgaagtagta cttcgactac aaaaataatc agagtaactg ctgatgtaaa
    22561 aaatcagttt taaaattgat cacaatcatt gtttaataac gattgaacta ttgcaaaaaa
    22621 caaatctgaa tcaatggatt ttacctatgg taatttccct gaatttacac gcaaagagtt
    22681 ttgcacattg tactaataaa atgtgagtac ctacactcga tttcaagaac actatggcat
    22741 tattttagct atttcatagg aaatcaaacg cagagttaag aaattagtat agagattcag
    22801 aatgggctgt aaaagttcaa ttgaagtctc aaccaatatt ctaaaaatca attttttgaa
    22861 tccgatggaa aaccataccg gttgcattgt aaacaaacgt atcgcaaccc caaaccgcaa
    22921 aaaaatccgg cgcctgcaat tatacaggcc tgcccggcac ctgcctcatc cccctgatca
    22981 tttgttttcc ggaaaattca acacgttaag catagctctg atacccttcg aataaatccg
    23041 tataaattcc tctgaaagat gaaaagactc ctagtagcag cactgattac ccttaccctt
    23101 ccctcattcg cgagagttta ctacacctcc ccgtcgggaa atgacaacaa tgccggatcc
    23161 attacagccc cctggtttac cttacaaagg gcctggactt ccgttgcagc aggcgacacc
    23221 gtttatatga gaggtggcac ctatacgtat actgttcagc cttacctgac cgggaaaaac
    23281 gggaccgcca gcaacctgat taagctctgg gcctatccgg gtgaatcacc tgttatcacg
    23341 cgtggaacct cctttaataa atcgaacggc tggcaccggg gaatggtatt tctgaccgga
    23401 aactatatcc atgtaaaagg aatccgtttt accgggatgt ataccgatga caaccaggta
    23461 gacgccggtt tacagactta taatgtaaat aattgcatat tcgagttact ggaatgtgat
    23521 aataatgtgg agggaatgat tgttgaaaat aacagtaata ataacctgat cctgaactgc
    23581 gatttccacg acaattacag caactacggt ggctcaaacg gggggaattc tgacggcctc
    23641 ggattaacct atatggtagg ccttaatacc gtgactacgg ttcgcggttg ccgcgcctgg
    23701 aataacgggg atgacggatt tgacactttt gaaaacagtg gctatgtact gatggattcc
    23761 tgctgggcct ggcacaatgg ctattttaaa ggaacaaaca ctgctgccgg agacggggtt
    23821 ggatttaaac tgggctcgga tttcctgact acccctgccg gggtgggtat tgtaaaaaga
    23881 aggctgcaac gcagtctcgg atttgacaat gcaaatgccg gagcacatat caatgaagcc
    23941 gacttcagca cagagctata caataatgtt ttcttccgga acaagattac cgggcttaat
    24001 ttccattata ataaccgggt acattatttc cgtaataatg tatcttttgg taataccgac
    24061 aaagacgtag aaatcagcgg aaacagtgtc cggagtaata actctgcagg aaccgctgga
    24121 aatgatggcg gttggacaac caatgcttcc gccgcagact ttatgagtac ggacactacg
    24181 ggtgtgcgta ataccagaca ggcgaacggc acgctgccga acctgaattt tctcaaattc
    24241 agttcaagca gcgacctgat agatgcgggt ttaaatattg gtcttccatt taatggtaca
    24301 gcgccggacc ggggtgccta tgagtttggt gtagccttac ccaaccagac tcctacagcc
    24361 aacgccggaa ccgataaaac catcacttta cccaccaatt ctattaccca aaccggatcc
    24421 ggaacagacc ccgatggcag tattgccgct tatcaatgga ccaaaatcgc cggtcccgcc
    24481 acttttacca tcgtctcccc cacccaggcc gctactgtta ttaataatgt cgttcaaggc
    24541 acataccagt ttgaattacg ggtgaccgat aatctcggtg ccacggcgag agacaccatg
    24601 atccttactg taaatgcagc cgccaatatt gcccccaccg ctaacgcggg tgccaaccag
    24661 accattacgt taccggtgaa tacagtaact gttaccggtt ctggtaccga tacggatggc
    24721 agtattgcga cttaccagtg gaccaaaatc gccggacccg ccacttttac catcgtctct
    24781 cctacccagg cctccaccgt tatcaacaac ctggtgcagg gcacctacca gtttgaatta
    24841 agagtgaccg ataacctcgg tgccattgga agagatacaa tgagtgttgt ggtgaatgcc
    24901 accaacaccc ctcccaccgc taatgccgga gccgatcaaa ccattacatt gccggcaaat
    24961 acggttacag taactggtag tggtacagac ccggacggaa gtattgcgag ctatgcctgg
    25021 acaaaaattt caggtccggt cactttcaat attgccacac ctaatcaggc cgccagtgtt
    25081 atcaacaatc tggtgcaggg tatttatatt ttcagtctta ccgttaccga taatgccggt
    25141 gcaacaggca ttgactatat tacaataact gtaaatgcag ccggtaatca gtcccctgct
    25201 gccaatgcag gtgcagacag aacgatcagt ttaccggtga attccctgac tgttaccgga
    25261 accggtacag accccgatgg cagtattgct ttctggcaat ggaccaaaat tgcaggacct
    25321 gttacattta atatcgtttc gcccacgcaa gcatctacgg tcatcaacag cctcgttcag
    25381 ggtgtttacc ggtttgaact gcgggtaacc gataacctcg gtgccgtagc aagggatacc
    25441 atgactgtaa ccgtgaatgc agcacccaac caggccccaa cagccaatgc aggcgcagac
    25501 cagattatca atctgccgac caacaccgta tccgtaaccg gcagcggtac cgatgctgat
    25561 ggatcaatcg cctcctatca atggacaaaa attgcaggac cggccacgtt taatatcgtt
    25621 tcacccacgc aggcgcaaac cacgataaat gccctggtac agggcagtta tcagtttgaa
    25681 ttaagggtta cggataatct cggcgctatt gaccgggata cgatgaccat caccgtgaac
    25741 ccccttccta accaaccgcc ggtagccaat gcaggagcag atcttacgat caccttacct
    25801 gttaacaccg ttactttaaa cggagttgcc accgatgcgg atggttccat cataacttac
    25861 cagtggacaa aaatttccgg cccggccaca tttaccattg tttcttcttc cacaccgcaa
    25921 actgcggtaa acggactggt acagggtatc tatgtattcc gcctgaccgt aaccgataat
    25981 aatggtgcct ccgctacaga tgatgtgacc gttaacgtaa atcctgcccc taaccaggcg
    26041 ccggtggcga atgcaggagc agacagaaca atcaccttac cgaccaacag tcttactgta
    26101 accggtacag gcactgatac agacggaaat atcgcttcct atcaatggac aaaaatttcc
    26161 ggtcccgcta ctttcagtat tacgtctcca acgcaggcgc agacgctgat cagtaacctg
    26221 atacagggca cttatcagtt tgaactgcgg gtaaccgata acctcggc
//
LOCUS       DAOPMW010000012        26093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1655_length_26093_cov_8.201628, whole genome shotgun sequence.
ACCESSION   DAOPMW010000012 DAOPMW010000000
VERSION     DAOPMW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26093
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_26093_cov_8.201628"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLU11_01310"
     CDS             complement(<1..382)
                     /locus_tag="PLU11_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57771.1"
                     /translation="MKKLLLLVLVMFALNAQSQSLKDLLFSGKMKTDSGTVVRKGEDL
                     KDKVDTATKKPAPVASEKAKPASINTDSVKTASVPVAAQAPANTEAVKPAEEAAPASP
                     QATATRDNNRIWKEFVDTVLGSIKA"
     gene            complement(737..2458)
                     /locus_tag="PLU11_01315"
     CDS             complement(737..2458)
                     /locus_tag="PLU11_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57772.1"
                     /translation="MKNVLLSFFSLMVLQSLNGQLTSDNIGFTRNGTSYSPFKGRFTA
                     VAIFPDGRILAGGEIDGPGRDYDFTLIAYNSQGIRDKSFGKDGIVQTDLEKFNDRLSS
                     ILMMSDGKFIAAGSANPYGKNTMGAMVKYEPNGSLDQSFGSNGMVRIPAGQQSLFVEK
                     ITQQKDGKILLTGTKRDGYGSRLVVYRFLTNGSPDPAFGNKGSVELPNINANAHSITV
                     LPSGKIAVTGVAGYSPSKYILVALLTSAGKWDPLFANKGVYEHRKTSYDESPGGLMAL
                     PDGRLMVTGSSINMNQLNMDSVLVFRLLANGKPDHSFGIRGDARYRFAARSNLAKAIL
                     LQPDQKILVIGTTSTDKNTRGMALRLHPNGNRDTTFGIRGIAILGFDQNTQDYIDAAT
                     LQPDGKLVLAGYSKSGMEWPAIGRLTANGEIDKGNPTPTVIKPPAPKTGTRLLNANEY
                     AELSRSMAGEFKRSIPGTIVAEGYLDGGNTIAKYKTITLNAAPNDVFLIAAFVKYSAP
                     VQIKIPGPNSYNITCNGDRITDAGATVHPYHCKYVNNTGQQRMIQFEINVQNSMEPGY
                     YLIIKAK"
     gene            complement(2544..3992)
                     /locus_tag="PLU11_01320"
     CDS             complement(2544..3992)
                     /locus_tag="PLU11_01320"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN57773.1"
                     /translation="MKKLFLLLLLPFQLVAQSGPDDLFNKIKQVETSLNPSLIFGDTL
                     PNNSIEQRLRETHVKGLSIAVIQNYKIHWAKAYGWADSSENRKVNLETRFQAASISKS
                     LNSMGILKLVQQGKLDTAADINQYLRNWKFPYDSLSKNKKITILNLLSHTAALDIHGF
                     PGYDRTDSFPKLTQVLNGERPANTKKIKSFGQPGKAYRYSGGGTTITQQMLMDITGAD
                     YADWMQKNVLKPLGMKNSSYRQPPTDTLNLATGYYGNDQPVHGKYHVYPEQAAAGLWT
                     TPSDLARYIIECQLTLNGKKGKVLSTPMMKTRMTPYIDKSAALGVFIEKKAGQPWFTH
                     NGGNEAFLCTSWGSMEGGNGVVIMINGEDFSVVNELLNSVATVYKWEGFFKPEFRKTI
                     SIPKDTLALYTGNFKMGKDTISLLMCGDHLCIQQNRQPAAGYKVFFTDKTHFSIREEA
                     ATSFTLLFNESGKLEALELKDPRMKMKLPKME"
     gene            complement(4040..4822)
                     /locus_tag="PLU11_01325"
     CDS             complement(4040..4822)
                     /locus_tag="PLU11_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57774.1"
                     /translation="MRWESPGKLVFSYLRNFLTRSSYGKYRHPIGHMMVELKDSTKHV
                     IAGVRAVKNSGMTRKVLFNGYGLGILFEKIQGKLDETDINLPDIQRRSKNGDIAFLEF
                     SISQAVFDRLWNYYNEYKEKGYHKLYNGLNQPLEGEGAGCSAFAFSFLEAAGLLDMIP
                     PEICRIDRTAPHSLVYQSGHKEAQVSLFKVLMSRSWSAENDAAAPLYSTYEPTWLFNW
                     IKRNHTAMPDKGKVQKTWLNKAPGILIDCSDQPVPQGPVWKN"
     gene            complement(4991..5740)
                     /locus_tag="PLU11_01330"
     CDS             complement(4991..5740)
                     /locus_tag="PLU11_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HPN57775.1"
                     /translation="MENIAGKYPDSWHYNHLYDPRMMSPGSNMPRYYGLMKAPLSYKV
                     ILQLLSEEYTTVTEADSLDIRQTLEMQANAVLQVLKRDNITPSSPVYNEAIALIAWLQ
                     YRPDTPERRHKDSLQKENSRRSEEQMMASLESDLSNPESQIMKLANSKDPAILKKGKN
                     YFSISCAVCHRENANGSIGPNLTDDYWLHDNSIKALFLTIFNGVPDHAMPAWKHSIDK
                     ERIATIIAFIRSLKGSNPPNPKEPQGEKRSD"
     gene            6070..7317
                     /locus_tag="PLU11_01335"
     CDS             6070..7317
                     /locus_tag="PLU11_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN57776.1"
                     /translation="MQPKQYGIFSLPVIVGALGFFVDIYDLLLFSIVRRSSFAELGVA
                     ESAMKSTGENIISWQMLGLTIGGILWGMMGDKKGRKSVLFGSILLYSFATIANGMAQT
                     IEQYTWLRFIAGLGLAGELGASITLTSELLPKEKRGIAAALIASSGVMGTIAAYFVHW
                     LSDENWRLCYYIGGAMGLALLLLRVGVLESGLFDASKKAKVAHGNFLHFFNNRDRFAR
                     YMRGILIGLPVWYIIGVLISFSDEFAKQFDITGFDQPTALMLQYVALLFGDMTAGLLS
                     NVIRSRKKTLLIFYAITAFFLAIFFITKGGGSATNMYLICMGLGFGSGISVLYITMSA
                     ETFGTNLRATAAISIPNLVRGFLPVILLIFQWLRSENVLNNYISGAWVTGLIVLLVGF
                     VSVLYTKETWGKELDFLEETGRK"
     gene            7492..8268
                     /locus_tag="PLU11_01340"
     CDS             7492..8268
                     /locus_tag="PLU11_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HPN57777.1"
                     /translation="MTKLLISLTLAITSICWISPVKKTPAIYQLTIFQYKDSVQEKTL
                     ETYFEQALLPALHKLEYNQVGVFKSWANDTSAVKKIYVLVAGKSMDELNGIKDKLAKD
                     GAYLDKGKAYLDAIYTSAPYYRIETIYLKAFSMAPELTLPALKGPKSERVYELRSYES
                     ATEKIFRNKVHMFNEGGEIALFKRLNFNAAFYSEVIAGSKMPNLMYMTCFENMTDRDA
                     HWKSFGSDPEWKRLSALPEYQHNVSLNEKNFLRPVDYSDY"
     gene            complement(8426..9655)
                     /locus_tag="PLU11_01345"
     CDS             complement(8426..9655)
                     /locus_tag="PLU11_01345"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPN57778.1"
                     /translation="MKQLPVLFLLAGSAILLNACIQSNGNPVTTTDGDQLPPVETRKA
                     NADYKPAFTGQTRISGAKTTTAYKVDKIGDKLGQPWAVVPLPDGQLLLTFKKGYMQIH
                     TTDGALVKKITGFPQVDDKGQGGMLDVTPDPAYSTNKTIYWSYSETSGNGNLMAVAKG
                     QLNEKNGTVDNPVVIFRATPALKSVLHFGSRLLFDKSGYLFVSTGERSISEGRAQAQL
                     LSSGLGKIFRITKDGKPAPGNPFAKQAGAIPEIYAYGIRNCQSLDIHPVTGDLWEAEF
                     GPRGGDELNIIKAGKNYGWPVITYGIEYSGQQVGEALQQKAGMEQPVYYWDPTLSPSG
                     MTFYKSKDIPEWENNLFIGGLSGQHIARLIIKNNKITGEERLLVDKNERFRDVAFYKG
                     MLFAVTDGGSLYRVSKK"
     gene            complement(9799..10641)
                     /locus_tag="PLU11_01350"
     CDS             complement(9799..10641)
                     /locus_tag="PLU11_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN57779.1"
                     /translation="MLLKDFIPAPELREFVQLYRIVHLRFEKGQVVPPKAYPPRPEHC
                     LAFYPYDTETVQYAESGKEIKHIPVVLYGQFTEVTHRMIGHHFLVFQIIFYPGALYRL
                     TGISAAELNNQYIDAEAVFSSELRRVNEQLYHAIGYPDMIRIANEFVHGLIKHQKKPR
                     LLTDHAWALLLTGNTKYSLDQLAREACLSTKQFERRFKERTGVNPKLYERIIRFDRAF
                     RLKNSKPEYDWLRIAVECGYHDYQHLVKDYKDFTSLSPTAFHEIENKAPERKFGLSEG
                     YYKE"
     gene            complement(10675..11232)
                     /locus_tag="PLU11_01355"
     CDS             complement(10675..11232)
                     /locus_tag="PLU11_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN57780.1"
                     /translation="MQRKQFLMAGLLAAPGLALARIPFKQLKHSIQGDPFIVGSGKSR
                     FGEVVKFLGVHPNDLKISSKDTGGQLSVFEYTGLGKVGPMLHIHFEQDEIFMVIEGEY
                     RFVVGEDTHVLKAGQTIFLPRGIPHTWIQLTERGKLIYFLQPAGKMEEFFSYMNKLTE
                     RPSPEEMDRIHAEHQMKVVGPPLTL"
     gene            11424..12644
                     /locus_tag="PLU11_01360"
     CDS             11424..12644
                     /locus_tag="PLU11_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57781.1"
                     /translation="MPLISKKGTFMLLLIFTVVFLVKCMDSKKISTDPRGVEYAGAGS
                     CRSCHQAVYDSFRLTSHHAATAPGDPALVKGSFLEGKNSFAFDDNRQVKMEKRGGRLY
                     QVLYKDGKEKEAHPFDISFGSKNAQTWLYWSGNNTYQLPISFYTSVNNWGTSPGYSIL
                     YPSFDRLIGRNCFECHSSHIGFLHGADSSPLTTNNPQKREEMDRRSLVTGIDCERCHG
                     PAREHVNYHAQNPGQSQGRFITRILGMSRQQQLDACAVCHSGNDRVKKKSRFAFRPGD
                     TLSHFFGSLMDSIIKTEPDVHGNQYGLLTESRCFIQSLQLTCNTCHDPHRDSPSNLAI
                     YSAKCVGCHPVGSPAFCTVQSPGADVLVQNCIDCHMPNQPSSAISFQLSGSTQRYSYY
                     LRTHRIGVYHLETK"
     gene            complement(12715..13164)
                     /locus_tag="PLU11_01365"
     CDS             complement(12715..13164)
                     /locus_tag="PLU11_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPN57782.1"
                     /translation="MKKLIILTGVIVVLASALSFISFQEDPWKVPEKYEKLTNPVPAD
                     EASIASGKELYDYFCLSCHGTDGKGSGKRAYKLNNTPTDFTLEIFQKQSDGAMLYKIY
                     SGHSDMPGFKKRIPGNQDAMEGSFGKTRIPGDLVNYIRTFGKKNNHH"
     gene            complement(13420..14700)
                     /locus_tag="PLU11_01370"
     CDS             complement(13420..14700)
                     /locus_tag="PLU11_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN57783.1"
                     /translation="MKRSALYCLAALAFLFTQPLAAQILNPKKILERKANRAIEKKTD
                     QAIDSLFDKKEKPKEVPQKTESPAPVPAEKPDPGKAADVIKKDTLPGLQLYSKYDFVP
                     GERVVFFDDFSQDNVGDFPALWNTNGSAEIVTSNLYPGRWMRYIGRTDIWTDSLLKLP
                     ENYTIEFDVIPIKGEEDRMAGYGFRLMQCINVRSWDGGSVPGKAGFNCGVEYFGRPYY
                     RTYINGEEGQDFGLTGFKDDHQYYQKENQKYHIAVWVQKSRVRYYVDENKLFDLPKAF
                     PLTSVKMDRIRFEDGAALVSNIRIAVGAPDMRNKLMTEGKIVSYGIYFDVNKDVVKPA
                     SYGSLKEIANILNENPEVKIKVIGHTDADGADAANLDLSKRRAAAVKNELVASFGIDA
                     SRIQTDGRGEAEPVAPNNSLANKALNRRVEFIKL"
     gene            complement(14811..16640)
                     /locus_tag="PLU11_01375"
     CDS             complement(14811..16640)
                     /locus_tag="PLU11_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPN57784.1"
                     /translation="MHPQLQKLLTCVNLEEKEQATRYALDQAHTLKQLKSEGLALHPL
                     IITRKNFGYADYPEISFRLAFPPETNLFRDGAAIECFISGEEPIKGVLISLEGKAGEF
                     RLFAPDFPDWIEDDGVGIKLAPDTRTTSIMKKVLNELEQHKSLFRLFEQIHGPAITAK
                     ANTTASGLSLTFKNSSLNQSQQEAIRAIVNNEDLLIVHGPPGTGKTTTLAEAIFQLVQ
                     QGEKVLVSAPSNAAVDHISKTLISLGINLLRVGNSSKTDAAVFAHTPEGKLGNSKSQK
                     EIKQLKIRAEEFRRMALKYKRSFGKAEREQRNLLFKEVKAIRAEIRKLQDYNEQKLFE
                     EAKVIAGTPIGLYDAGVGQLKFNTLFLDEAGQCIEPLAWCIFPMAEKIVLAGDHWQLP
                     PTVLSHQAAAMGFNNSILEVAIPHTGAVYLLDTQYRMRESIAGFSSRYFYKELLKTAI
                     HLENRGTHITFIDTAGSGFNEKQGPDGISLQNEGELQIIRQFLEQEAWDPAGTAFISP
                     YSGQVAAAKEMLPAVMRISTIDSFQGQEKEKIILSLVRSNDDGDIGFLKDYRRMNVAI
                     TRAKEQLLVIGDSATIGGDKFYQSFLEYVEEKGHYRTVWEFQL"
     gene            16787..17620
                     /locus_tag="PLU11_01380"
     CDS             16787..17620
                     /locus_tag="PLU11_01380"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPN57785.1"
                     /translation="MALVCNRLAGSGLSLEVMARLEGLLHQYNIPKTVFIQDWPDSFD
                     GFSDIYLVGGDGTLNYFINKYPDITIPLVIFKGGTGNDFHWLLYRDVDMVSQFKQVLD
                     LPARPVDCGRCNNRLFINGVGAGFEGAVAKSLSAKKKLPGKTSYLLTILQKIFSYRSR
                     HFTIMTGDKVITGKKLLVDISNGRRAGGGFHIAPEARANDGLLDLVLADALSSLRRLI
                     YLPVIEKGKHLGLSIITHLPVRSVRIQSLEIIQYHLDGEYAEATELNITIREKAFLFR
                     Y"
     gene            17685..18215
                     /locus_tag="PLU11_01385"
     CDS             17685..18215
                     /locus_tag="PLU11_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN57786.1"
                     /translation="MTIFSDPHIGFAGTTDIPAIMELLNSAYRGESSRKGWTTEADLI
                     AGNVRTDEQNLTDVMAIPGSVFLKYTNEDGQLQGCVNLQVQDQRVYLGMFSVHPGLQG
                     TGIGKIILRSAEEFALSRDCTRIYMSVISVRAELIAWYQRHGYADTGERKAFKEDGLT
                     GKHLRELEFMTLEKPL"
     gene            18258..19235
                     /locus_tag="PLU11_01390"
     CDS             18258..19235
                     /locus_tag="PLU11_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN57787.1"
                     /translation="MVLLDVRGIGKVEKTGFSVNDIHFSQEQYQQIAIVGETGSGKTS
                     LLKMIAGLIQPDSGEILFEGNRVLGPLEKLLPGHPRIAYLSQHFELRNNYRVEEELEA
                     RNKRTEEEAQAIYEVCRITHLLKRRTDQLSGGERQRIVLARLLTTSPSLLLLDEPYSN
                     LDRHHTQVIKAVLKDLVEQWKISCIMVSHDAEDLLSWADRVLVMREGRIIQDGSPETI
                     YEQPLDSYTASLLGEFNLLDHSLAAVLLPGSEIPADKKWMLRPEQLNLDRTDHSPVAA
                     VIREMHYCGSHYLAHAEVMGQELKIRSSIRLETGVTVGLSLLPGNPWFI"
     gene            19257..19616
                     /locus_tag="PLU11_01395"
     CDS             19257..19616
                     /locus_tag="PLU11_01395"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HPN57788.1"
                     /translation="MKTIFFLLFSLTGITTFGQQKIKPISVADSTKKIQVVETACGQC
                     QFGLKEKGCLLAIRIKGQAYFVDGTTIDEHGDAHAEDGFCNAIRKSEVQGTVVKGRFN
                     ATYFKLLPPAAVKKEQQ"
     gene            19709..19936
                     /locus_tag="PLU11_01400"
     CDS             19709..19936
                     /locus_tag="PLU11_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57789.1"
                     /translation="MTIKKTMPAYRMILGAFFLTAVMAVGCNEGTEKKEPVNDSPNVT
                     APVPPVTDTPAPPIDTSKMKEASTRPVKTTD"
     gene            complement(19983..21485)
                     /locus_tag="PLU11_01405"
     CDS             complement(19983..21485)
                     /locus_tag="PLU11_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN57790.1"
                     /translation="MNQRDFTLLYRSLRCLLVFCLFLQSVTVSAQQSNVDSMRNLLRL
                     ESVDTARVKLLWQLARDMGGYNPDTAQYLSTQALYLARKINYLEGESRALGVLANTLV
                     KIGNYPRALELNIQKLQIEEKRNKPRNLASVLMNIGVVYVLQEEYPKALEYYRKADSV
                     IRSSNVEDMKYYINLNIGDVFNRVDISDSAYNYFSTSLAIARKLGDGDLIGTSMTGLG
                     HTYLKQGNYQASALNYRTGIGLLTAAKDDEILCEALLGLANLFEKTDQRDSASFYANA
                     SRQLAKNAGFLSKELEAAEFLTQHFQLERSIDSAFMYVSYVRGLNDSVNSKSRIRESQ
                     VITSNEQYRQMEMEEQKRLERAQRFKQLQMLLIAIFIPGFFLITLVLSRVNVHIKVIR
                     VLGVLSLLFLFEYLTLLLHPTVADITHHTPVLEILIFVAIAAILIPLHHKLEHWFIHK
                     LIHHRHGEAVKKEAAAAAALAEKESQQPATITADPEKPSATPETPGSPKK"
     gene            21619..22944
                     /locus_tag="PLU11_01410"
     CDS             21619..22944
                     /locus_tag="PLU11_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPN57791.1"
                     /translation="MNRLLRLCLLFSGLFAAVSSYSQTLYEMRYSYTSDGVTDRLSAF
                     ILRNDDGTGLVRVAYLNSEDNKNKLVEMEMQESYGQHEDGTEDSSQLVFVGLNPRFII
                     GTPDDEFLEDHFVFSFNPDSGFYEPSFVFSMPDDTSTQVGQLEEVRFLNQEDLTQELV
                     LQFFTEQDVFYKQLFETSTRGLSADDKQAQLHLVLVANTEDKDIGKTCVIDKDATYKT
                     FSEIAEFLEINFKPTVIAGKEFSKVNVDKAVSSVRPGPNDIVVFYYSGHGFNEVDEQY
                     QFPYLDLRDKSFQKYGGAYTMNVEAIYQKLRAKGARLNLVISDCCNKDPTSGALVSSE
                     GASTRTSSIGWNKENCQALFMSKQPMSIMMTAAQKGELSAGNPSDGGIFTFIFREQLE
                     KALGPFTRNPSWNNLVQSAKKETVNKARTTWCNKEKRIVCVQNPVFKME"
     gene            23022..24896
                     /locus_tag="PLU11_01415"
     CDS             23022..24896
                     /locus_tag="PLU11_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPN57792.1"
                     /translation="MKKIGHTGTGYNLYPVIFLFFISMVAANAQSLRVEPMNWWVGMK
                     NQQLQLMVYGEGIGSSTPVLAYPGVKLEKVHKAESPDYLFLDLRINAGTKPGSFNIRF
                     NKQGSTVATYNYQLAARNKERAAVKGFSPADVVYLITPDRFANGDPSNDVVEGMREKK
                     INRTNDYGRHGGDIRGMINNLDYIASMGFTAIWPTPLLENDMPNASYHGYAITDFYKV
                     DPRFGTLEEYKELADKARQKGLKLIFDGVVNHTGSQYWWMKNLPFKNWINYPDSIRIT
                     NHRRTVHQDLYASASDRELMVKGWFVRQMPDMNQDNPYLSTYLIQNSIWWVETLGLAG
                     IRQDTYPYSQKKFLEQWSCQIMNEYPGFSIVGEEWSTNPVIAAYWQKGKPNTTGYNGC
                     LKSTMDFPIQSALMQALTEPESWDKGLVKLYEALANDFVYASPKDLLVFGDNHDMDRV
                     FTSLKKNPELMQMAMSYLLTIRGIPQIYYGTELLMENSAKPGDHGLIRSDFPGGWTED
                     TLNGFTGAGLTAEQLAAQSYLKKLLNWRKNKSVIHSGKTLHFGPANGVYVYFRYTEKE
                     TVMVILNKNAGPTGLHTDRFAEILKGKKTAMDVIRGNQYPLSEELIIPGRSALILELK
                     "
     gene            complement(24967..>26093)
                     /locus_tag="PLU11_01420"
     CDS             complement(24967..>26093)
                     /locus_tag="PLU11_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPN57793.1"
                     /translation="GITAFSGRTISINVGLFGSSPQVSSTMPTPGGNISGTTKDERGM
                     SVMPNLAMVWSKKNSKSHFGVSAYGISGFGVTFPESATNPVTMPQQSGGFGQVKSDYQ
                     LLQVGLTWAYQLNDKVSVGVAPTFNYSALELLPNPLSSPSPTKGYPGADKATALGIGG
                     QAGIYFNSGTGFKMGASYKSQQYFQDFNFKNYYADGSQAPNVDFKMNYPAIASAGLGY
                     TNKLFDFALDYRFIDYKNTKGFDTKGWTPTASVAGFGWENISVVSAGVQYKALKAMPF
                     RIGYTYSSNPITSELAMFSLPATAIIKHAFQFGFGFEAGKKLTLNGVFHHGSSGGSLD
                     GALLHPGWITNSNPYGAVPGSKISYKMTTNMLMFGVNYKL"
BASE COUNT         6698 a         5847 c         6185 g         7363 t
ORIGIN      
        1 cggccttgat gctgccgagt acggtatcca caaattcttt ccagatgcgg ttattgtcgc
       61 gcgtggcggt ggcctgcgga ctggcagggg ccgcttcttc agccggtttt acggcttcgg
      121 tattggcagg cgcctgggct gcaacaggta cggatgcagt tttcacactg tctgtgttta
      181 tcgatgctgg ttttgctttc tcgctggcta ccggagcggg ctttttagtg gcggtgtcca
      241 ctttatcctt caggtcttct cccttgcgga ctaccgtgcc tgaatcggtt ttcattttgc
      301 cactgaagag caggtctttg agggactggc tctgggcatt caacgcaaac atcaccagta
      361 caaggagaag gagttttttc atatttcaag attttgggcc agggccttac tgcaatttac
      421 aaatcctttc gttagggagt tcttaaaaat tgccacggga tgcgctaaag cgcttatata
      481 gttggccacg aaaacacacg tagcggcacg aaaaatacag cgcaacaggt tgagctgaag
      541 cacaaaaaaa tacagggcca cggatttcac gcctgtcccg ccttatgcgg gagataaaca
      601 cggattgatt gcctacggca aaaaaggtag ccacgaaatt ccacgaaggg actcgaaagt
      661 aatacaaaga gtttacggct gtttaattcg tgccctttcg tgtgttttcg tggccaacta
      721 tgccgcgaca cggcttttat ttagctttta tgatcagata atacccgggc tccatgctgt
      781 tctggacatt gatctcaaat tgtatcatcc gctgttggcc ggtattgttt acatacttgc
      841 aatgataggg atgtacagtg gcaccagcat ccgtgatgcg gtctccgttg caggttatat
      901 tatagctatt ggggcctgga attttaatct gaacaggagc ggagtattta acaaaagccg
      961 cgatcaggaa aacatcattg ggggctgcat tcaatgtaat ggttttgtat ttggcaatcg
     1021 tattgccgcc atcgaggtat ccttcagcca caatcgtacc cggtattgat cttttaaatt
     1081 cacctgccat ggatcttgac agctctgcat attcattggc attgagcagt cttgttccgg
     1141 ttttgggtgc cgggggtttg ataacggtcg gtgtcggatt gcctttatcg atctcgccat
     1201 tggcggtaag tcttccgatg gccggccatt ccattccgga ttttgaataa ccagccagca
     1261 ccagtttccc atctggttgt aaagtagccg catcaatata atcctgtgtg ttctggtcaa
     1321 atcccaggat ggctatcccg cgaataccaa atgtggtatc ccggttacca ttggggtgca
     1381 ggcggagggc catcccacgt gtatttttat cagttgatgt ggttccaatc accagaattt
     1441 tctgatcagg ttgcagcagt atcgctttag caagattact cctggcggca aaccggtagc
     1501 gggcatctcc ccggatgcca aaactgtgat cgggttttcc attggccagt aaacggaata
     1561 caagtactga atccatgttt aactgattca tattgatgga tgagccggta accatcagtc
     1621 ggccatccgg taatgccatc aatccaccgg gtgattcatc ataagaggtt ttgcggtgtt
     1681 catatactcc tttgttggca aacagtggat cccatttgcc ggctgaggtg agtaaggcga
     1741 ccagtatata ttttgaaggt gaatatccgg ccactcctgt cacggcaatt ttaccggagg
     1801 gcaatacagt tatactatgt gcattggcat ttatattggg caattctacc gaccctttat
     1861 tgccgaaagc aggatcggga ctgccatttg tcaggaaacg gtaaacaacc agccggctgc
     1921 catatccgtc tctcttcgtg ccggtgagta aaattttacc atccttctgc tgcgtgattt
     1981 tttctacaaa taaactttgc tggccggccg gaatccgcac cattccatta ctgccaaaac
     2041 tctgatccag gctgccgttg ggttcatatt tcaccatagc gcccatcgtg tttttcccgt
     2101 atggattggc agaaccggca gcaatgaatt ttccatcgga catcatcaga atggaactta
     2161 accggtcgtt gaatttttca agatcagtct gcacgattcc atcctttcca aaacttttat
     2221 cccggatacc ctggctgtta taagcaatca gggtaaaatc ataatctctg ccgggaccat
     2281 caatttcccc gccagccagt atccggccat cggggaaaat cgcgacagcc gtaaaccggc
     2341 ctttgaacgg actatagctg gtaccgttgc gggtaaagcc aatattatca ctggtaagct
     2401 gaccattcag cgattgaagt accatcaggc tgaaaaagga aaggaggaca tttttcatgg
     2461 ttcctttttt gattattcta aagataatca tccgtctttt ttccatgaat accttggatt
     2521 gggtattttg gcccttctcc ggtttactcc atttttggca gtttcatttt catccgtgga
     2581 tccttgagtt ccagggcttc cagttttccg gactcattaa ataaaagagt aaatgaggtg
     2641 gcggcttctt cccggataga gaaatgggtc ttatcggtaa agaacacttt gtacccggca
     2701 gcaggctgac ggttttgctg aatacaaaga tgatcgccgc acatcaacag tgaaatagta
     2761 tcttttccca ttttgaaatt tccggtatac aacgccaggg tgtcttttgg aatggagatg
     2821 gttttacgaa actccggttt gaaaaatcct tcccatttat atacggtggc cacactgttc
     2881 agcagttcat tcaccacgga aaaatcttct ccattgatca tgataaccac accatttccg
     2941 ccttccatac tgccccagga agtacagaga aaggcttcat taccgccatt atgagtaaac
     3001 catggctgtc cggccttttt ctcaataaac acaccgaggg cggcgctctt gtcgatataa
     3061 ggcgtcatcc ttgttttcat catcggggtg gacaacactt tacctttttt cccattcaac
     3121 gtcagctgac actcgatgat ataacgggcg aggtcactgg gagtggtcca tagtcccgct
     3181 gccgcttgtt ccggatatac atggtatttc ccatgcaccg gctgatcatt gccgtaatag
     3241 ccggtggcca ggttcagggt atcggtggga ggctggcggt acgaactgtt tttcatgccg
     3301 agtggcttca gcacattttt ttgcatccag tctgcataat cagctcctgt aatatccatc
     3361 agcatttgtt gtgtgatagt tgtgcctccg ccggaatagc ggtaggcctt cccgggttgt
     3421 ccaaaggatt ttattttttt ggtattcgcc ggtcgttcgc catttagtac ctgggtcagt
     3481 ttagggaaac tatcagtccg gtcataaccg ggaaagccat ggatatccag tgcagcggta
     3541 tgactgagca ggtttaatat cgtgatcttt ttgttttttg acagactgtc gtaaggaaac
     3601 ttccagttgc gcaggtactg attgatatca gctgcggtat ccagtttgcc ctgctgtacc
     3661 agtttgagga tgcccatact gttcaggctt ttactgatcg aagccgcctg gaaacgggtt
     3721 tccaggttca ctttccggtt ttctgacgaa tcggcccagc cataggcttt ggcccaatga
     3781 attttataat tttgaatgac ggcaatgctg agtcctttca catgggtttc ccggagtcgt
     3841 tgttcgattg aattattggg caatgtatcg ccaaaaatca gggaagggtt cagggacgtt
     3901 tcgacctgtt tgattttatt gaaaagatca tcgggtcctg attgggcaac taattgaaaa
     3961 ggcaatagaa ggagcaggaa gagttttttc atgactggtt tttgtggccc taaagttagg
     4021 ggatcggcta aacctgttat tagtttttcc aaaccggccc ttgtggaacc ggctgatcgc
     4081 tgcaatcgat cagaatacct ggtgctttat tcagccaggt tttctgcact ttgcctttgt
     4141 cgggcattgc tgtatgattc cgcttaatcc agttaaacaa ccaggtgggt tcgtaggtgg
     4201 aatagagtgg ggctgctgcg tcattttccg cagaccagct tctggacatt aaaactttaa
     4261 acaaggacac ctgtgcctct ttatgaccgg actgatatac caacgagtga ggggctgtcc
     4321 ggtcgatacg acatatttca ggaggaatca tatccagtaa tccggcggct tcgagaaaac
     4381 tgaaagcaaa ggcggagcaa ccggcccctt cgccttcgag cggttggtta agcccgttat
     4441 ataatttatg ataccctttt tctttgtatt cgttatagta attccagagc cggtcaaaaa
     4501 ccgcttgact gatgctgaat tcaaggaagg caatatcccc gtttttggaa cgtcgttgta
     4561 tgtccggcaa attgatatcc gtttcatcca gtttgccctg aattttttca aataaaattc
     4621 caagaccata accgttgaac aacactttgc gcgtcatgcc cgaattttta accgcacgca
     4681 caccggcaat gacatgcttt gtactgtctt tcagttccac catcatatga ccgatcggat
     4741 gccggtattt tccataggaa gaccgggtca gaaaattccg gagataactg aagaccagtt
     4801 tacccggcga ttcccaacgc atttgatgcg gtgcaggaat agtataaatg gtgagtgtct
     4861 gggctttaat tttatacggg tagaatagac ataggagggc taaaattata attacccccg
     4921 attttttatg aaaagtgccc gccattggta caatatactt ttaaccagcg gcacttacta
     4981 atgattttgc tcaatctgag cgtttttcgc cctgtggctc ctttggattt ggaggattgc
     5041 tgcctttaag tgaacggata aaagcgatta tagtggcaat tctttcttta tctatgctat
     5101 gtttccaggc tggcatggca tgatccggta ctccattaaa aatggttaaa aaaagtgctt
     5161 tgatactatt gtcatggagc cagtaatcat cagtcaggtt tggcccgatg gatccgttgg
     5221 cattttcacg atgacagacg gcgcaactga tcgaaaagta gttttttccc tttttcaaaa
     5281 tggccggatc cttgctgttg gccagtttca taatctggct ttccggattt gacaagtctg
     5341 attcaagtga ggccatcatc tgctcttcgg atctgcggct attttctttt tgaagagagt
     5401 ccttatgacg acgttccggg gtatcgggcc ggtattgcag ccaggcgatt aaggcaattg
     5461 cttcattata tacaggagag gagggtgtaa tattatcacg tttaaggact tgcaaaacag
     5521 cattagcttg catttccagc gtctgacgta tatccagact gtccgcttct gttacggtag
     5581 tgtattcttc acttagcaat tgaagaatga ctttatagct caacggggct ttcattaatc
     5641 catagtaccg tggcatgttc gagccggggg acatcatgcg gggatcatat aaatggttat
     5701 aatgccagga gtccggatac ttaccggcaa tgttttccaa acttattttg ttactgtcaa
     5761 gtaatggggt acatgtatga catttattgc aattttcctg ttggtaaatg tatttcccat
     5821 aggcagtcag gagattaaat gaagtgtcgt aatttgatct ggttctatat gaccactttg
     5881 tttgttttgg taaaacaaac gagaagaata aaagagggag taacaacagg atcaggataa
     5941 agcttttttt catgagagaa aagtacaaat aatccaattt atatcgcatg attcagcacc
     6001 gggattccat tgttcagtga atttttcttt agcaaagctt tcttatcttt ccgcacaaac
     6061 cagcgattga tgcaaccaaa acaatatggc attttctccc tgcccgtaat cgtgggcgcc
     6121 ctgggttttt tcgtggatat ttatgatctt ctgcttttca gtattgtccg ccgttccagt
     6181 tttgccgaac tgggtgtagc cgaatctgcc atgaaatcca ccggcgaaaa catcatcagc
     6241 tggcagatgc ttggactcac tatcggtggt attctttggg gcatgatggg ggataagaag
     6301 ggacgtaaaa gtgtgctctt tggttcgatc ctcctttatt cctttgccac cattgccaat
     6361 ggaatggcgc agacaattga acaatacacc tggctgcggt tcattgccgg actgggattg
     6421 gcgggtgaac tcggggccag tatcaccctc acgagtgaat tattacccaa agaaaaacga
     6481 gggattgccg cagccctgat tgccagcagc ggggtgatgg gtacgattgc ggcttatttt
     6541 gttcactggc tgtccgatga aaactggcgg ctctgttatt atatcggtgg cgcaatggga
     6601 ctggccctgc tcttattacg cgtgggggta ttggaaagcg gtttatttga tgcgtcaaaa
     6661 aaagccaaag tggctcatgg taatttcctt cattttttta ataaccggga tcgttttgcc
     6721 cgctatatgc ggggtatcct gatcgggttg ccggtctggt atattatcgg ggtactaatc
     6781 agtttctccg atgaatttgc caagcagttc gacatcaccg gatttgatca gcctaccgcc
     6841 ctgatgctcc aatatgtagc tttacttttc ggtgatatga cggcgggact gctaagtaat
     6901 gtgatccgga gccgcaagaa aaccctcctg atcttttacg cgatcactgc ctttttcctg
     6961 gcgattttct ttatcacaaa aggcggcggc tcggccacca atatgtacct gatctgtatg
     7021 ggactcggtt ttggctccgg tatttccgta ttatatatca ctatgagtgc cgaaaccttc
     7081 ggcaccaatt tacgagccac ggccgccatc tctataccca atttggttcg tggttttctg
     7141 cccgtcattc tcctgatctt ccagtggctg cgcagtgaaa acgtgctgaa taactatatc
     7201 agcggagcct gggtcaccgg cctgatcgta ttgctggtag ggtttgtgtc ggtgctttat
     7261 actaaggaaa cctgggggaa ggagctggat tttctggaag aaacgggaag gaagtaaaaa
     7321 gtcaaaagta aaaagtaaaa attgcaggtt gaaagattga aaatttattc ctgactgttc
     7381 atttctgcct ttgctcggag cttctttata taaatgtttt ttctgctttc gtatttttta
     7441 ctttttactt tttacttttg acttaccaca tcgctacttc agaaattaat tatgacaaaa
     7501 cttctaatat cactcaccct tgccatcaca tcaatttgct ggatatctcc tgtaaagaaa
     7561 accccggcga tctaccagct gacaatcttt cagtataaag acagtgtaca ggaaaaaacc
     7621 ctggagacct attttgaaca ggcgttactt cctgcattgc acaaactgga atataaccag
     7681 gtgggcgtct ttaagtcctg ggccaatgat acatcggctg ttaagaaaat ctatgtgctg
     7741 gtggccggga aatcaatgga tgaactgaat ggaattaagg acaaactcgc gaaagatggc
     7801 gcgtacctcg ataaagggaa agcatacctg gatgcgatat atacaagcgc tccatactac
     7861 cggatagaaa cgatatacct gaaagcgttt tccatggctc cagaactgac cttgcctgct
     7921 ttgaagggcc cgaaaagcga aagggtctac gaactgcgta gttatgaaag tgccacggaa
     7981 aagatcttcc ggaataaagt acatatgttt aatgaagggg gagagatcgc cctttttaaa
     8041 aggttaaatt tcaacgccgc cttttacagc gaagtgattg ccggcagcaa aatgcccaac
     8101 ctgatgtaca tgacttgttt tgaaaacatg accgaccgcg atgcacattg gaaaagcttt
     8161 ggcagtgatc cggaatggaa aagattatcc gccctccccg aataccagca taatgtatcg
     8221 ctaaatgaga agaatttttt aagaccggtt gactactcgg attattaagt aaaaattaaa
     8281 aagtaaaaat agcaggctga aagattgaaa ctacattcct ggcacttcat ttcttccaat
     8341 ttttgaagat tcaatataaa atgatttttc tgctttagaa ttttttactt tttacttttt
     8401 aattttgact tcccgacttc ttcccctatt tcttgctgac cctgtacaaa ctccctccat
     8461 ccgtaaccgc aaacaacatt cccttataaa atgccacatc gcggaagcgt tcgtttttat
     8521 ctaccagtaa acgttcctct ccggtgattt tattgttttt tatgatcagg cgggcaatat
     8581 gttgtccgct gagtcctccg ataaaaagat tattttccca ttcaggtata tccttgcttt
     8641 tgtagaaagt cataccactg ggggagaggg tggggtccca ataatatacg ggctgttcca
     8701 tcccggcttt ctgctgcagg gcttcaccta cttgctgtcc gctgtactca atgccatagg
     8761 taatgacggg ccagccatag ttcttgccgg ctttaatgat attcagttca tcgccaccac
     8821 gtggaccgaa ctctgcttcc cagagatcgc cagttaccgg atggatatcc aggctctggc
     8881 agttgcggat tccataggca tagatctcgg ggatggcacc ggcttgtttg gcaaagggat
     8941 ttccgggagc gggtttgccg tctttcgtga tacggaatat cttacctaag cctgaactga
     9001 gtaactgggc ctgtgcccgg ccttcggaaa tggaacgttc accggtgctg acaaagagat
     9061 aaccgctttt gtcaaataat aaacgggaac cgaaatgcaa gacacttttc aaagcgggtg
     9121 ttgccctgaa aatgaccacc ggattatcca ctgtcccgtt tttttcattg agttgtcctt
     9181 tggctactgc catcaggtta ccgttaccgg aggtttctga ataggaccag taaatggttt
     9241 tattggtcga atatgccgga tccggtgtta catccagcat gccaccctgg cctttatcat
     9301 ctacctgggg gaatccggtg atttttttca ccaatgcacc atcggtggta tgaatctgca
     9361 tatacccttt cttaaaagtc agcaacaact gtccatcggg caggggtacc acggcccagg
     9421 gttgcccgag tttatcaccg atcttgtcca ccttataagc agtagtggtt ttggcaccac
     9481 taatccttgt ctgaccggta aatgccggtt tataatccgc attggctttc cgggtttcca
     9541 ccgggggcaa ttgatcgccg tctgtagttg tgacaggatt gccattgctt tggatacagg
     9601 cattcaaaag gatggccgat ccggcaagta aaaaaaggac aggtaattgt ttcatgtttt
     9661 caatgttgaa gcgtaaaaat aatgaactct gttctaacaa agcatgggat taattgttta
     9721 ggcggaaggt tcaatgcgac gtgaagaaca aacagataaa atatactgaa cggtgtggtc
     9781 tccgggtcgt acgtattctt attctttgta atacccttca ctcagcccga attttctttc
     9841 cggggcctta ttctctattt catgaaaggc ggtcggggag aggcttgtaa aatccttata
     9901 gtcctttacc aggtgctggt aatcgtggta accgcattca accgcaatcc ggagccagtc
     9961 atattccggc ttcgagtttt ttaaacggaa ggcccggtcg aatcggataa tccgttcata
    10021 cagttttgga ttgacaccgg ttcgttcttt aaaccggcgt tcaaactgtt ttgtactcag
    10081 acaggcttcg cgggcaagct ggtcgagact gtattttgta tttccggtta ataataacgc
    10141 ccaggcatgg tcggtcagca agcggggctt tttctgatgc tttatcaatc catgcacaaa
    10201 ttcattggct atccgaatca tgtccgggta accgatcgcg tgatacaact gttcattaac
    10261 tctccttagt tcactggaaa aaacagcttc tgcatcgata tattgattgt tcagttcggc
    10321 agctgaaatc cccgttagcc ggtacaaggc accaggataa aaaataatct ggaaaacgag
    10381 gaaatgatgc ccgatcatcc ggtgggtaac ttcggtgaac tgaccataca ggacaacggg
    10441 aatatgtttg atttctttac cggattccgc atactgaacg gtttccgtat cgtaaggata
    10501 aaatgccagg caatgctcgg gccgtggcgg ataggccttg ggaggcacta cctgtccttt
    10561 ttcaaaccgg aggtgcacga tccggtagag ctgtacaaat tccctgagtt caggggctgg
    10621 tatgaaatct tttagcagca aacttttagg ttctatagtt aaggtactgc ttttttataa
    10681 tgtcaggggt ggacctacaa ctttcatctg gtgctccgca tggatccggt ccatttcttc
    10741 gggactggga cgttcggtca gtttattcat ataggagaaa aattcttcca ttttcccggc
    10801 gggctggagg aaataaatca gtttcccccg ttcggtcagt tgaatccagg tatgtggaat
    10861 accacgggga aggaaaatgg tctggcctgc ctttaaaaca tgtgtatctt ctccaactac
    10921 aaatcggtat tcaccctcaa taaccataaa aatctcatcc tgttcaaaat ggatatgcag
    10981 catcggaccc actttgccca ggccggtgta ttcaaaaaca gaaagttgtc cgccggtatc
    11041 tttcgacgag attttgagat cattgggatg aacacccagg aacttcacca cttcgccaaa
    11101 ccgggatttg cccgaaccga caataaaggg atctccctgg attgagtgct tcaattgttt
    11161 aaatggaatc cgggccaatg ccagtccggg tgcagccagt aatccggcca tcaggaattg
    11221 tttacgttgc atacgattgg tttttgtact gtaaaaatga agccggatgg ttataggtta
    11281 gtggtaaaaa aaagacattt tcagggatag ctgcagataa tggatttgtt attgatctga
    11341 aagtgtcagg aagtattcat taactggtaa ctgatgttca attgtcggcc agcaggttaa
    11401 ctgcttttcc ctaaattgca ggtatgcccc tgatttccaa aaaaggaact tttatgctgc
    11461 tattgatttt caccgtagta tttctggtga aatgtatgga tagtaaaaaa atctccacag
    11521 atccccgggg agtggagtat gccggtgcgg gttcctgccg atcctgtcac caggccgtat
    11581 atgattcatt ccggcttact tcccatcatg ccgctacggc tcccggtgac ccggcccttg
    11641 taaaagggag ttttctggaa gggaaaaaca gttttgcctt tgacgataac cggcaggtga
    11701 aaatggaaaa aaggggagga cgtttgtacc aggttttgta taaagacgga aaagaaaaag
    11761 aggctcatcc ttttgatatc agttttggca gtaaaaatgc acaaacctgg ctgtattgga
    11821 gtggcaataa tacttaccag ttacctattt ccttttatac ttcagtcaat aactggggca
    11881 ccagtccggg ctattccatt ctttaccctt cttttgaccg gctgatcgga cgaaattgtt
    11941 ttgaatgtca tagttcccat attgggttcc tgcatggagc agatagcagc ccgttaacca
    12001 cgaacaaccc gcagaaaaga gaagagatgg atcgtcgttc actcgttacg ggaattgact
    12061 gtgaacgttg ccatgggccg gcccgtgagc acgtaaatta ccatgcgcaa aatccaggtc
    12121 agtcgcaagg ccgctttatc acccggatct tgggtatgag cagacagcag caactggatg
    12181 cctgtgcggt ttgtcattca ggcaatgacc gggtaaaaaa gaaatcccgt tttgcattcc
    12241 ggccgggtga taccctttct catttcttcg ggtcactgat ggacagtatc atcaaaaccg
    12301 aaccggatgt acatggcaat cagtacgggc tgctgaccga aagccggtgt tttatccaaa
    12361 gcctgcagct gacttgtaat acctgtcatg atccgcaccg ggattctccg tctaacctgg
    12421 cgatctattc tgccaaatgt gtgggttgtc atccggttgg cagtccggct ttctgtaccg
    12481 ttcaatcacc tggagccgat gtcttagtac aaaactgtat tgactgtcat atgcccaacc
    12541 agccttcgtc tgccatcagc tttcagttgt ccgggtccac ccagcgttat tcttattatt
    12601 tgcgtaccca cagaatcggg gtgtaccatc tggaaacgaa ataaactcaa cgaatgaact
    12661 aaaaaaaatg ccagcatcag ctgacatttt tttttaattg actatcggac atgctcaatg
    12721 gtgattattt ttctttccga atgtccggat atagttcacg aggtcgccgg gaatcctggt
    12781 tttaccaaag cttccttcca tcgcatcctg atttcccgga attcttttct tgaaaccagg
    12841 catatcgcta tgtcctgaat aaattttgta aagcatagcc ccatcggatt gtttctggaa
    12901 aatttccagg gtgaaatcgg taggggtgtt attcagttta tacgcccgct tgccggaacc
    12961 tttaccatca gttccatgac aggacaaaca gaaatagtca tataactctt tgccggaagc
    13021 aatagaagct tcatcggcag gtacagggtt tgtcagtttc tcatatttct ccgggacttt
    13081 ccagggatct tcctggaagg aaataaaaga cagcgcggag gccagtacaa cgataacgcc
    13141 ggtcagtatg attagctttt tcatttttaa tgtgtttgta agatcatcaa taaaatttcg
    13201 tcagttaaaa gtagaccgga gtggtatgtg gttattatga cgcctgtcaa agcctttaat
    13261 gcgtttgctc agtttttgtc agcaggtaaa ttacagacat gtcttttaat aggaatgaaa
    13321 gtcaatgcca gccttgatta catcgatttg taaaacgaaa tgaccaactg gtgacaacca
    13381 tttttcttta aaattaactg tagtagtatt gtttactgct tacagtttaa tgaattccac
    13441 ccgccggttc agggctttat tggccaggct gttattgggc gctaccggtt ctgcttcacc
    13501 acgtccatcc gtttgtatcc gggaggcatc aatgccaaaa gatgccacaa gttcattctt
    13561 caccgccgcg gcccttcttt tggaaagatc caggttggcc gcatcggctc catcagcatc
    13621 cgtatgacct atgactttga ttttcacttc cgggttttcg ttcagaatat tggcaatctc
    13681 tttgagcgaa ccataggaag ccggttttac cacatcttta ttcacatcaa agtatatccc
    13741 atagctgaca atctttcctt ctgtcataag tttgttacgc atatccgggg caccaaccgc
    13801 gatccggata ttactgacca gtgccgcacc atcttcaaac cggatccggt ccatttttac
    13861 agaagtcagt ggaaaagctt ttggcagatc aaataattta ttttcgtcta cataatagcg
    13921 tacccttgat ttctgcaccc atacggcaat atggtatttc tgattttctt tttggtaata
    13981 ctgatgatca tctttaaaac cggtaagccc aaaatcctgt ccttcttccc cattaatata
    14041 ggtccggtaa tagggtcttc cgaagtattc aaccccgcaa ttaaaaccag ctttgccagg
    14101 tacagaacct ccatcccagg aacgaacatt gatacattgc atcagccgga aaccatatcc
    14161 cgccatccgg tcttcttctc ctttgatcgg gatcacatca aattcaatgg tataattttc
    14221 tggcagttta agtaaggagt ctgtccagat atccgtacgg ccaatatacc gcatccagcg
    14281 accgggataa agattactgg taacgatttc tgctgagcca ttggtattcc ataaggcggg
    14341 gaaatccccc acattgtcct gcgaaaagtc atcaaaaaat accacccgtt cacccggcac
    14401 aaaatcatac ttggaataca attgcagtcc gggcagggta tctttcttta taacatccgc
    14461 tgcctttccg ggatccggtt tttctgcagg aaccggtgcc ggcgattcag ttttttgcgg
    14521 aacttctttc ggcttttcct ttttgtcaaa caggctgtct atcgcctgat ccgttttttt
    14581 ctctatcgcc cggttggctt ttctctcgag gatttttttt ggatttaata tctgtgccgc
    14641 taaaggctgg gtaaacaaaa atgcaagggc agccaggcag taaagtgcag accttttcat
    14701 aaatgtgtat tttggaaaag gtaatccgct ggcgaaggag gttcctttga taagggtcat
    14761 tcattcccgt gattataatc aaaagcccgc ttaccgatgg aagtattgaa tcagagctga
    14821 aattcccata ccgtacggta atgtcctttt tcctccacat attcgaggaa ggactggtaa
    14881 aacttatcgc ccccgatcgt ggcactgtcg cctattacca gcaactgctc tttggcccgg
    14941 gtgattgcca cattcatccg ccggtaatct ttcagaaaac cgatatcacc atcatcatta
    15001 ctccgaacca gtgacaaaat gattttttct ttctcttgtc cctggaaact gtcgatcgtg
    15061 ctgatccgca tgaccgcagg gagcatttct ttagccgcgg ccacctgccc tgaatacggg
    15121 gatataaatg ccgtgcccgc cggatcccat gcttcctgtt cgaggaattg ccggatgatc
    15181 tgcagttcac cttcattctg taaactgatt ccgtccggac cctgtttttc attaaatccg
    15241 gagcctgctg tatctataaa tgtgatatgg gtgcctctat tttccaggtg aattgctgtt
    15301 ttcagtaatt ccttataaaa atagcggctg ctgaatccgg caatggattc ccgcatccgg
    15361 tattgggtat cgagcaggta aacagcacct gtatgtggaa tggccacttc gaggatggaa
    15421 ttattaaaac ccatggcagc tgcctgatga ctcaataccg tgggtggtaa ttgccaatga
    15481 tcaccggcca gcacaatctt ttctgccatc gggaagatgc accaggccag gggttcaata
    15541 cattgtccgg cttcatccag gaacagggta ttgaatttta actgacccac tccggcatca
    15601 taaaggccaa tcggggtgcc cgcaatcacc ttggcttctt caaagagttt ctgttcattg
    15661 taatcctgca gtttacggat ctccgcgcgg atggctttaa cttccttgaa caaaagattc
    15721 cgttgttccc gttccgcttt gccaaaactt cgtttgtatt ttaaggccat ccgtcgaaat
    15781 tcttcggccc ttatcttgag ttgtttgatc tctttctgcg atttgctgtt acccagtttg
    15841 ccttcggggg tatgtgcaaa gacagcggca tccgttttac tgctgttgcc cacccgcagc
    15901 aggttaatcc ccagtgaaat cagggttttg gagatatgat ccaccgcggc attactggga
    15961 gccgatacca atactttttc tccctgttgc actaactgga aaatagcttc cgccaatgtg
    16021 gtggttttac cggtgcccgg agggccatgg acgatcagca ggtcttcatt atttacaatg
    16081 gctcggatcg cttcctgctg actctggttt aaggaactgt ttttaaatgt aagggaaagg
    16141 ccggatgctg ttgtattggc ttttgctgtg atggccggtc catgaatctg ttcaaaaagc
    16201 cggaacagtg atttatgctg ctccagttca ttcagtactt tcttcatgat agacgtggta
    16261 cgggtatccg gcgccagttt gatacctacg ccgtcgtctt cgatccagtc cggaaaatca
    16321 ggggcaaata accggaactc acccgctttc ccttccaggc ttattaaaac tcctttgatt
    16381 ggttcttccc cactgataaa acattcaata gcggcaccat ccctgaacag gttggtttcg
    16441 ggagggaatg ccaaccggaa actgatttca gggtaatcgg catatccgaa gtttttacgg
    16501 gtaataatca acggatgtaa ggccaggcct tccgatttca gctgttttaa ggtatgggcc
    16561 tgatcgagtg cataacgggt ggcttgttct ttttcctcca ggttcacaca ggtcagcaat
    16621 ttttgcaatt gggggtgcat ggccggaaga taagtaaata gcccatgggt gaaatgataa
    16681 tttttcagat tgactgaaga aaattatctt ccgttctctg ttgctttaaa cggcagtaaa
    16741 acagtttatt cacctcaacg attagaacct ggttcaggaa agaaatattg ccttagtctg
    16801 taaccggctg gctggttccg gcctttcact ggaagtaatg gcccggctgg aaggactgtt
    16861 gcaccagtac aatatcccta aaacggtttt catccaggac tggccggatt cctttgatgg
    16921 cttttctgat atttacctgg tcgggggtga tggaacgcta aattatttta taaataagta
    16981 ccctgatatt accatccccc tggtgatctt caaaggggga accggaaatg attttcactg
    17041 gctgctttat agagatgtgg atatggtcag ccagtttaaa caagtattgg atttacccgc
    17101 ccgcccggtg gattgtggcc gttgcaacaa ccggttattt ataaatggag tgggggccgg
    17161 gtttgaagga gcagtagcca aatcactcag cgccaaaaaa aagctacccg ggaaaacctc
    17221 atacctgcta acgattctgc agaaaatttt cagttatcgc tcccggcatt ttactatcat
    17281 gaccggtgac aaggtaatta ccggaaaaaa attgctggtg gatatcagta acggacgccg
    17341 ggccggaggc ggttttcata tcgcacctga agccagggcc aatgacggac tgctggatct
    17401 ggtgttggct gatgctttgt cttccttacg ccggctgatc tacctgcccg ttatcgaaaa
    17461 agggaaacac ctgggactgt ccattattac ccatttgccg gtcaggtctg tacgtattca
    17521 gtcactggaa attatccaat accacctgga tggggaatat gcggaagcca cagaactgaa
    17581 tataacaatc agggaaaagg cctttctttt ccgttactga atatttcaat tttgccggcc
    17641 atcacaagca aaaccagttc ccccatttct tttaattttg cgccatgact attttttctg
    17701 atccgcatat agggttcgcc ggtaccacag atataccggc tattatggaa ttattgaata
    17761 gtgcctaccg gggtgaaagt tccagaaaag gctggactac cgaagcggac ctgattgccg
    17821 ggaatgttcg tacggatgaa cagaatctga ccgacgtgat ggcgattccc ggcagtgttt
    17881 ttctgaagta tacgaatgaa gacggacagc tgcagggttg tgtcaatctg caggtacaag
    17941 atcaacgtgt ttatctcggc atgttcagtg tgcatcccgg attgcaaggg acgggtatcg
    18001 gaaaaatcat tctccgttcg gctgaagaat ttgccctttc ccgggattgt acccgtattt
    18061 atatgtctgt gatctctgtc cgggcagaac tgattgcctg gtaccagcgc catggctatg
    18121 cggataccgg agaacggaag gcttttaagg aagacgggct taccggcaaa caccttcggg
    18181 aactggaatt tatgaccctt gaaaagccgc tctgacctgt tgcttgcagt aaagcggcga
    18241 tgacctattt tcggggcatg gttttattag atgtcagggg gattgggaaa gttgagaaaa
    18301 caggtttttc tgtaaatgat attcatttct cccaggaaca ataccagcag attgccattg
    18361 tgggcgaaac cggctcaggt aaaaccagcc tgctcaaaat gattgccggt ttaatacagc
    18421 cagattccgg tgagatactt tttgaaggca accgggtatt aggtcctttg gaaaaactgt
    18481 tacccggtca tccccggatc gcttatctca gtcagcattt tgaattgcgg aataattacc
    18541 gggtcgaaga agaactggaa gcccggaata aacgcactga agaagaagcg caggctattt
    18601 atgaagtttg ccggatcacg cacctgctta aaagaagaac ggatcagtta tctggtggag
    18661 aacgtcaacg gattgtgctg gcccgcttac tcacgacttc cccgtctttg ttattactgg
    18721 atgagcctta ttccaatctc gaccgtcatc atacccaggt gatcaaagcg gtgttgaaag
    18781 acctggtgga acaatggaag atcagttgta tcatggtctc ccatgatgcg gaagatctct
    18841 tatcgtgggc cgatcgggta ctggtaatgc gggagggccg aattatacag gatggtagtc
    18901 cggagactat ctatgaacag ccacttgata gttataccgc ttccttactg ggtgaattta
    18961 atctgctgga tcattcatta gcggccgttt tacttccggg cagtgaaata cctgctgaca
    19021 aaaaatggat gcttcgtccc gaacaactga acctggacag aacggatcat tccccggtag
    19081 ctgccgtcat ccgtgaaatg cattattgcg gcagtcacta tttggcccat gccgaagtta
    19141 tggggcagga attgaaaatc agatcttcca tcagattaga aacaggagta acggttggcc
    19201 tatctttatt acccggcaat ccctggttta tttaataaaa acaaaaacag acaacgatga
    19261 aaacaatctt cttcctatta ttcagcctga ccggaattac cacttttggt cagcaaaaaa
    19321 taaaacccat ttccgtggcc gacagcacca aaaaaataca ggtggtggaa acagcctgtg
    19381 ggcaatgtca gttcggatta aaagaaaaag gatgcctgct ggctatacgc attaaaggcc
    19441 aggcttattt tgtagatggt acaacaatcg atgagcacgg tgatgcgcat gcggaagatg
    19501 gcttttgcaa tgctatccgt aaatctgaag tacagggtac tgttgtgaaa ggccgtttca
    19561 atgccactta ttttaaactg ttaccgccgg cagccgttaa aaaagaacag cagtaaacag
    19621 caaagcccgg taacctgccg cctattcatc cttatttttt ggaaaatccc caaaaattct
    19681 gtaaccttag tacttaaacc aaaaacatat gaccataaaa aaaacaatgc ctgcttaccg
    19741 gatgatcctg ggtgcattct ttctgactgc tgtgatggcg gttggttgta atgaaggcac
    19801 agagaaaaaa gaacctgtta atgattcacc taatgtgacg gctcctgtgc ctccggtgac
    19861 ggatacgcct gcgcccccga ttgatacatc aaaaatgaaa gaggcttcca cccggccggt
    19921 aaaaactacc gactaagtgc aggcttaaaa tgattgaaag gtttccggtt tacgggaacc
    19981 ttttattttt tgggggaacc cggagtctcc ggtgtagcgg aaggcttctc cggatcggcg
    20041 gttatagtgg ccggttgttg cgattccttt tctgccaggg cagcggccgc cgctgcttct
    20101 tttttcaccg cttccccatg ccggtgatga atcagtttat ggataaacca gtgctccagt
    20161 ttatggtgca gaggaatgag tatggcggca atggccacaa aaatcagaat ttccagcacg
    20221 ggggtatggt gagtaatatc agccacggtg gggtggagga gtaaggtcag gtattcaaaa
    20281 agaaacagaa gagaaagcac acccaatacg cggatgactt taatatgcac atttacccgg
    20341 ctaagcacca gggtgatcag gaagaaaccc ggaatgaaaa tcgcaatcag caacatctgc
    20401 agttgtttga aacgttgcgc cctttccagt cttttctgtt cttccatttc catttgtcgg
    20461 tattgttcgt tgctggtgat cacctgtgac tcacggatac ggctcttgct gttgaccgaa
    20521 tcgtttagcc cccggacata actgacatac ataaaagcac tgtcaatact ccgctccagt
    20581 tggaaatgct gggtcagaaa ttcagcggct tccagttcct tgctcaggaa tccggcattt
    20641 ttggcaagct gcctggaggc attggcataa aaactcgcgg aatcgcgctg atctgttttt
    20701 tcaaacaggt tggccagtcc cagtaaagct tcacatagta tttcatcgtc tttggcggcg
    20761 gtcagcaaac cgatgcccgt gcggtaattc agggcagacg cttgataatt accctgtttg
    20821 agataggtat gacccaggcc ggtcatggaa gtgccgatca ggtcaccgtc tcccagttta
    20881 cgggcgatag cgagggaggt actgaaataa ttataggctg aatctgaaat atccacccgg
    20941 ttaaacacat ccccgatatt aaggttgata taatatttca tatcctccac attactggag
    21001 cggatcactg aatcagcttt ccggtaatat tcgagggctt tggggtattc ctcctgtaaa
    21061 acatatacca ccccgatatt catcagtaca ctggccaggt tcctcggctt attccgtttt
    21121 tcttcaatct gtagtttctg gatattgagt tccagcgccc gtggatagtt gccgattttc
    21181 accagggtat tggcgagtac acccagggcc ctggattcac cctccaggta attgatttta
    21241 cgggccaggt atagtgcctg cgttgacagg tattgtgcag tgtccgggtt atatccgccc
    21301 atatcacggg cgagttgcca gaggagtttc acccgggccg tatccacact ttccagccgc
    21361 aacagattgc gcatactgtc cacattcgac tgctgcgcac tgaccgttac tgactgaagg
    21421 aacaggcaaa aaacaaggag gcagcggagt gaccggtata gtaaagtgaa atcccgttgg
    21481 ttcatgagtc tgtcttaatc gttctaattt atagaataaa aacggattac gcatttttta
    21541 gacgtcaacg ggttgaataa ttttttgggt aaaccggatg aatttggtag atttaatttc
    21601 acctcaaacc tgctgctgat gaatcgttta ctccggctgt gcctgttgtt ttcaggactg
    21661 tttgctgccg tttcctccta ttcccagact ttatatgaaa tgcgctattc ctacaccagc
    21721 gatggggtta cggaccgctt aagtgcattt atattgagga atgatgatgg aaccggtctg
    21781 gtcagggtgg cttatctcaa ttccgaggat aataagaaca aactggttga aatggagatg
    21841 caggaatcgt atggccagca tgaggatgga acggaagaca gttcacaact ggtatttgta
    21901 ggccttaatc cacgattcat tattggtacg cccgatgacg aattcctgga agatcatttt
    21961 gtgttcagct ttaatccgga ttcgggtttc tatgaacctt cttttgtttt ttcaatgccg
    22021 gatgatacct ctacccaggt aggtcaactg gaggaagtac ggttcctgaa ccaggaagac
    22081 ctgacccagg aactggtctt gcagtttttt acagagcagg atgtttttta taagcaactt
    22141 tttgaaacca gtacccgtgg actcagtgcg gatgataagc aggcccagct gcacctggta
    22201 ctggtggcca atacagaaga taaggatatc gggaaaacct gtgtgattga taaagatgcc
    22261 acctataaaa ctttcagtga gattgccgaa ttcctggaaa taaattttaa accaacggtg
    22321 atcgccggta aggaattcag taaggtcaat gtggataaag cggtcagttc agtacggcca
    22381 gggcccaatg atattgtggt cttttattat tccgggcatg ggtttaatga ggtggatgag
    22441 caataccagt ttccttacct tgatctgcgg gataaaagtt tccagaaata tggaggggct
    22501 tataccatga atgtggaagc catttaccag aaactccggg ccaaaggggc ccgtctcaac
    22561 ctggtgatca gtgattgctg taataaggat ccgacatccg gggctctggt cagttcagaa
    22621 ggggcttcca cccgaacctc ctccattggc tggaataagg agaattgtca ggcgctgttt
    22681 atgtccaaac aaccgatgtc cattatgatg acggctgcac aaaaaggcga attgtctgcc
    22741 ggcaatcctt ctgatggtgg aatattcact tttattttcc gggaacaact ggaaaaagca
    22801 ctaggaccct ttacccgtaa ccccagctgg aacaacctgg tgcagtccgc taaaaaagaa
    22861 accgtaaata aagcacgtac aacctggtgc aataaagaaa aaagaatcgt ttgtgtgcag
    22921 aacccggttt tcaaaatgga ataaacggtc ttttttccgg tgtatagatt ccctgcagat
    22981 attctaatga ttaaaatatc tttggggaat aatctcagta tatgaaaaag attggccaca
    23041 caggaaccgg atacaacctg tatccggtta tttttttgtt tttcatttca atggttgccg
    23101 caaatgccca gtcgctacgg gtagaaccca tgaactggtg ggtaggtatg aaaaatcagc
    23161 aacttcagtt gatggtgtac ggggagggaa tcggatcttc cacacctgta ttggcttacc
    23221 ccggggtgaa actggagaaa gtacataaag cggaaagccc tgattatctt tttttggatt
    23281 tgcggataaa cgcaggtact aaacccggca gttttaatat ccggttcaat aagcagggaa
    23341 gtacagtggc tacctataat taccagctgg cagctaggaa caaggagcgt gcagcggtga
    23401 aaggcttttc cccggctgat gtggtttacc tgattacccc tgaccggttt gccaatgggg
    23461 atccttccaa tgatgtggtg gaggggatgc gggagaaaaa aatcaaccgg accaatgatt
    23521 atggccgcca tggcggggat atcaggggga tgatcaataa tctggattat atcgcttcta
    23581 tgggttttac ggctatctgg cccacaccct tactggagaa tgatatgccg aatgcttcat
    23641 atcacgggta cgcaattaca gatttctata aagtggatcc ccgctttggt acactggaag
    23701 agtataagga attagccgac aaagcccgtc agaagggctt aaagctgatt tttgatgggg
    23761 tggtgaatca taccggttcg cagtactggt ggatgaagaa cctccccttt aaaaactgga
    23821 ttaattatcc ggactccatc cggattacca accaccggcg tacggtacac caggaccttt
    23881 atgcttcagc gtccgacagg gaattgatgg taaaaggctg gtttgtaaga cagatgccgg
    23941 atatgaacca ggataaccct tatctgtcca cttacctgat ccagaacagt atctggtggg
    24001 tggagaccct cggtttggcc ggtatacgtc aggataccta tccctattct cagaaaaaat
    24061 tcctggagca gtggagttgc cagatcatga atgaatatcc aggattcagt attgtaggag
    24121 aggagtggag taccaacccg gttattgcgg cttactggca aaaaggaaaa cccaatacaa
    24181 ccgggtataa cggctgcctg aagagtacta tggatttccc tattcaatct gcgctgatgc
    24241 aggcgctgac cgaaccggag tcatgggata agggactggt gaaactctat gaggccctgg
    24301 ctaacgattt cgtatatgcg agcccgaaag atcttctggt atttggcgac aaccatgata
    24361 tggaccgggt gtttacctca ttgaagaaaa acccggaact gatgcagatg gcgatgagtt
    24421 atttactgac gatccggggg atcccgcaaa tctattatgg aacggaatta ttgatggaaa
    24481 attcggctaa accgggtgac cacggactga tccgttccga ttttcccggc ggatggacag
    24541 aggataccct gaatggattt accggggcgg gattaacagc agaacagtta gccgcacaat
    24601 cttacctgaa gaaattatta aactggcgga aaaataaatc cgtgattcat tccggaaaga
    24661 ccctccattt cggaccggct aacggggtat atgtttattt ccgctataca gaaaaagaaa
    24721 ccgtgatggt gatcctgaat aaaaatgcgg gaccaaccgg gttgcatacc gatcggtttg
    24781 cggagatttt aaaaggcaaa aagacggcga tggatgtgat ccggggaaat cagtatcccc
    24841 tcagtgagga attgattatt ccgggccgtt cagccctgat tcttgaatta aaataaaata
    24901 gcgtcatctc ttttataaaa aaagccccgg atttccgggg ctttttttat aaagtaattt
    24961 ctgaaatcag agtttgtagt ttacgccaaa catcagcatg ttggtggtca ttttatacga
    25021 gatcttactt ccgggaacag ctccgtaagg attggagttg gtgatccagc ccggatgcag
    25081 taaggcgccg tcgagtgaac cgccgctgct gccatgatgg aacaccccat tcagggtcag
    25141 tttcttaccg gcttcaaatc caaaaccaaa ctggaatgca tgtttgatga tggcggtggc
    25201 gggcaaagag aacatggcca gttcggaagt gatcgggttt gagctgtagg tataaccgat
    25261 ccggaatggc attgccttta aagctttgta ttgtacaccg gcggacacaa cggaaatatt
    25321 ttcccagcca aatccggcta cggaagcggt gggcgtccat cctttagtgt cgaatccctt
    25381 cgtgttttta taatcaataa accggtagtc cagtgcaaaa tcaaacagct tgtttgtata
    25441 accaagtccg gcagaggcaa tggccgggta gttcatttta aaatccacat tgggtgcctg
    25501 ggagccatct gcgtagtaat ttttaaaatt aaaatcctgg aaatactgct gggatttata
    25561 ggaagccccc attttaaaac cagtacctga attaaaataa atacccgctt gtccgccgat
    25621 acctaaagcc gtggctttat ctgctcccgg gtatcctttg gtaggactag gtgaagaaag
    25681 cgggttgggt aataattcaa gcgccgaata gttaaatgta ggagcaactc caacagaaac
    25741 cttatcattt aactggtaag cccatgtcag tcccacctgt aataactggt aatccgattt
    25801 cacctgaccg aaaccgccgg attgctgggg catagtaacc gggttagtcg cactttcggg
    25861 gaaggttact ccgaaaccgc tgatcccgta agcagataca ccaaaatggc ttttactgtt
    25921 tttcttagac cataccatgg ccaggtttgg cattacagac atcccccttt catcttttgt
    25981 tgtgccggaa atattaccac ccggtgtagg catggtggaa cttacctggg gtgaggagcc
    26041 gaataatcct acattgatgg aaatagttct tccggaaaag gcggtaatac cag
//
LOCUS       DAOPMW010000013        26027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1663_length_26027_cov_11.107693, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000013 DAOPMW010000000
VERSION     DAOPMW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_26027_cov_11.107693"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLU11_01425"
     CDS             <1..236
                     /locus_tag="PLU11_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57794.1"
                     /translation="GASVTLRDGKQRHGSGDDRALIILGSDLQNIFVADDPAHKPTGR
                     FLLQQLGDFRFKFDKSFDACQRDKNTCSISSKR"
     gene            complement(248..613)
                     /locus_tag="PLU11_01430"
     CDS             complement(248..613)
                     /locus_tag="PLU11_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57795.1"
                     /translation="MMNEKAFRAIYSNYLESGLTIRDFCTNQSIHESKFFYWQNKLKG
                     VLPPKRGFVPVIFSSNQQKPSPELPVAMNNQPEQYNKHASSVMSLTCEINYPNGIRVK
                     VNGLSDLEMVRTLLASTQQ"
     gene            682..823
                     /locus_tag="PLU11_01435"
                     /pseudo
     CDS             682..823
                     /locus_tag="PLU11_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457950.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(965..3322)
                     /locus_tag="PLU11_01440"
     CDS             complement(965..3322)
                     /locus_tag="PLU11_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57796.1"
                     /translation="MTMRSHEAAVFGYVVRIIDSENENVDLIYNYRTSYSTFDLVYGE
                     EVTSISARSDFEQLSREWIPIELRYNYREQSLLLVLPDTTLAAYPVKFSQKVKILFGA
                     SDFENFKTTDVPPMNIRDITLRHEGQLIHEWKLNESAGNIAFDSKGKVNALAMNPVWL
                     KPKFQFWAKNADILLEGNGEVAFNQKDEVVYIIGDELLMVYSVKENRLDTVRYLNKPD
                     VLKQGCQAFYDPVHRSVVSYNVDLKSIARFNLTTRLWEQTASPAYPLTVFWHHNKYFQ
                     EKDSTLYIFGGYGQHEYKNSVLRFRFNENRWDTLITSDKLFHPRYLSASGMNNDTVFI
                     LGGYGSLTGKQILNPGTYKDLVAYSIQHNHFSLIREFDPPMEDIAFANSMVIDPDHQE
                     FYVLAFPIFRYEGYLQLMKGRLNSPELTLAGDKIPYLFHDIQSFSDLFLCQTSWKLVC
                     VTLLKTENNKTQAQVYTIGYPPGSLTDIEKTSGWNKNLLFLFPALFLVSWLLFLAVEK
                     KMGTRRSTVSQESSTDQKLSEIQDISSAADLFTNIHPKSNTILFLGDFQIFNRTGSDI
                     TSKFSPLLKELFLLIWLNSIKDKGISAERIIEILWLDKDEKSARNNLAVNIVKLKTLL
                     SELDSIELSKSTGYWKSIFNEETVYNDYWHCLRTARHYGQPGRDDICELIRISQKGHF
                     LESLSFDWLDEFKAELSNIIIDTLIRYSDQMDTSRDPNLALHLAHTVLLYDMLNEEAI
                     LLKCKTLTNQGKHHLAKEAFDKFTRDYKNLYNIPYPKTLLEILNF"
     gene            3941..5092
                     /locus_tag="PLU11_01445"
     CDS             3941..5092
                     /locus_tag="PLU11_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71/99-like protein"
                     /protein_id="HPN57797.1"
                     /translation="MAGYQGWFNADGDGAGRGWNHYWKNQRLAPGSCKFDYWPEMSEY
                     PVQYNSPFYFSDGKQATLFSSYDESTVDLHFKWMKEYGIDGVFIQRFVTNLNNPVSYG
                     HNQKVLASAFKAAERHGRILCIMYDLSGIAAGEETLLMEDWKKLVEQYQLKNENRLDN
                     YLCHNGKPLVVVWGAGFNDNRKYGLKEVRTIVDFLKNDSLYGGFSVMLGVPTYWRTLK
                     NDTQSDTLLHTIVRSADIVQPWFVGRFNEETYLGFKDLLRDDMAWCKENGPDYVPVVF
                     PGFSWHNMYPNYPSNQIPRNKGQFYWKQLAGVLSLGAEMVYVAMFDEIDEGTAIFKCA
                     HQVPVGESVFVPLDEEIPSDHYLWLTGQAGRMLRKEIPVSAEMPMKTHN"
     gene            5125..8208
                     /locus_tag="PLU11_01450"
     CDS             5125..8208
                     /locus_tag="PLU11_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN57798.1"
                     /translation="MISLLLMWCVPVRAQVLSVQGKVTDQNNAGLPGASIVVKGTTVG
                     TITDINGNYLLNVPNPEQAVLVFAFIGYDMLEIPVRGQSTINAVLSTSNIGLEEVVVT
                     AMGITRDAKALSFARQSVSVEEMTEARSPNIVNSFSGKIAGVQVVPSGFNTGSSRIVI
                     RGNSSLTGNNQPLFVVDGVPIDNSPGDSGSIDYGNNAADLNSDDIESIEVLKGPNASA
                     LYGSRAANGVILISTKKGTPKFKITLNSGTMFQTLTQFPEYQNAYGVGTSFYIDNQHM
                     LPWANVNYRSWGSPMLGQPYVALNGEVKPYLPQPDNVKNFYQKARLLTNSVALEGGNA
                     DNIVRFSYTNYNGNSVVEGFNKNTKHNVNLRVQNKFRKWITLDTKVNFIRDNVVNRQY
                     SNDNGRNPSYMYAHMARSTNLDELIPWKDELTGKEIGTHRNFSNPYWVIHENPNEDTK
                     DRVIATFNPEIKITDWIKLIGRFGADMFWTNGYEFNNIGSIIASNPNGFLRTFNVNQQ
                     NINLEGILSVNKKLEAFSVSANFGLTRFDSRSERRDSRINSLLQPGLINLSNAKEYPV
                     VSQNQSRKRINSAFGALSFGYKDFAFVDLTGRNDWSSTLPSKNNSYFYPSIGGTLIIT
                     ELLKAKSNLLSFAKLRASYAIVGNDSDPYRLDQTFSFNGFFNDATVASLSTTMNNPDL
                     KPEKTTSYEYGLDLRFFKNRLALDATYYDASTVNQIITASLPTSSGFERRLYNAGEIK
                     NWGYELSLTGQPVQRGNFKWETRINFSKNNSEVVSLLDDIPRFQLANRSSYLYVYAEV
                     GKPYAYLRGLGVKRDNQGRMLLEDGGGLLQKNTDMPFGTASPDWLAGIGNTFSWKGLD
                     LYFLVDIKQGGVMYSGTISRMLTNGMFAETLEGRDDFYLHSVIWGENTSELTGGARWD
                     AYFNDGTKNTKFMTPQNYEYARPNFAEFVIYDASFVKLREVSFGYYFPKLLLAKTPFE
                     NARISATGRNLWILHSNTPQGIDPEASSTSGNGQGIENGALPPNAIYGINVRLIF"
     gene            8220..9803
                     /locus_tag="PLU11_01455"
     CDS             8220..9803
                     /locus_tag="PLU11_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPN57799.1"
                     /translation="MKSKLIYIAIMVGLFSCTDQFEEINQNPNNPVKVEPEFLLTTSL
                     RETQNLYGGNMNGVVFYNYTHYFSGFQGEFQRYTYSVSSTNDYWRNNYVRCLQPVHQI
                     VKLYEEDPSYKNRVIIAKIWKAYIFSNIVSVWGGVPMSAALEGTPGVAYEKEQDIYTA
                     LLDQLKELAEAIDLTGDTYKANADKIYGGDLMKWKKFAQTLRLRLAMRISAADPEKAK
                     KIALEISRNATGIISSKAETAAMNWGITSDSWSYLYNRVVYNYTANKATIPVLCESLV
                     YHTLPYNDARLPVYGQPAKQGPSIGKYFGQNISYGGGGGYTTRTNPHSLLKQDDYSYI
                     GERFLQPDAEYVFLSYAEACFLKAEAALKGWWKDTNAQNYYYEGIDASYDHYGLSADQ
                     ATAYKETPGIKWGTETDSTGRFYEFQDWMGICSSYIKANDFNRQIIMQHWLAMPNQGV
                     DAWALIRRTRILEFQPQFATYDGEYAYIPDRIPYPGSEYSTNPLEVEKAVSWLGGPDG
                     LFTKLWFGLPNVKNPNLPH"
     gene            9836..10891
                     /locus_tag="PLU11_01460"
     CDS             9836..10891
                     /locus_tag="PLU11_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPN57800.1"
                     /translation="MKNIFYALVGLFLWVSCEETDEHLPPQFNYEIPPTELSDDVLVG
                     ALYYNYATADWAKKYTNTPQLGQYSALSAETMKQHRIWADQAKINFFIFPWNGTSGNA
                     LLTSFITGRSENVKMVLNYSTAHLSATNASPLAGAKLTTMVNEFKSHYITHFDKEYYF
                     KIDGKPVVIISPVNLASSAAASIDYPMVITALRTELKNDGTDLFLIGEITSGWLPPQR
                     YATALKTFDAVVLSNWTANGNYGYDRSVFYPAFSDQAFKNWADSTSVWGIDFVPCIMP
                     GFDDKVMTPASKNFNIVRSAKLYTDMCNVAKRNLGEKKITIINSWNNFQFGTSIEPTS
                     EYGTEYLEITSKQFKVQ"
     gene            10955..12040
                     /locus_tag="PLU11_01465"
     CDS             10955..12040
                     /locus_tag="PLU11_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN57801.1"
                     /translation="MKRFIITIIIVLFIFSEALPQKSSMIVLGDLHYDLLEDHDMEWL
                     RTKPDDLRQVTKEYTVYTDKYWNDFMAVLLQKAEAVRPPLRAVVQLGDLSEGLAGSPE
                     KAKQMAGNTMKAIEKVGMEVPWILAKGNHDITGPGAPEAFQEFYVPVIREQTGNPEIQ
                     NASYTYSFDNVSVTCIDPWERGFSMVDFLDQELTKSAALVKFVAIHEPVIPVTERCWH
                     TMRRNPEQRVKLLEVIARHKAIVLCAHLHRYSVVKRNTPWGPIVQVMVISVIKDKDYQ
                     KPAKVITQYGPSLAEAFPDWQPETLEARKAMLAEEAKYVTFYKQTDLPGYAVFKINAK
                     KRKVDLEYYAAFGAKPYDKLAISNLMK"
     gene            12180..13313
                     /locus_tag="PLU11_01470"
     CDS             12180..13313
                     /locus_tag="PLU11_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN57802.1"
                     /translation="MFFFKRKFFTLLLGFIVTCGQSQDISFIALGDLHYDRLDLHDWD
                     YVMKRPQDFEQIMKEYPQITAVYLPKFLRLIKEQSLVITPPVKAVVQLGDLVEGVAGN
                     ERLAREMNRGVVDLLYSVEMAVPWILVKGNHDVSNSPGQPEAWQEVIRPFIEGQVNSP
                     VGRGMYTHRLTGQVELFVLDQFFSVDQNLPETAMLSFLEEVFSKSEARFKFVLTHQPV
                     IPVTDRCWHLMSGIRRPLSDPRLRERLLNLLAKNKAIVLCAHLHKYSVVSRKTEAGAV
                     VQVMINSVVGSFDLKASVEVETEYKGAGFVEENPTFQLHNQEVRKKILENEKPNIQYF
                     TRADLSGYAIISVYEKENKVVLKYYNGFSADPFQSLELSAWWE"
     gene            13431..14657
                     /locus_tag="PLU11_01475"
     CDS             13431..14657
                     /locus_tag="PLU11_01475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPN57803.1"
                     /translation="MRRRFLLVLFWTVLSCIPSWGQPEGVHLSLSATGKGEKQFMTVL
                     WYSREAGAVQFIRYGLTEKELPKMVKADSYGMEGSFFHKGEMTKLTSSTRYYYQCGSD
                     KSGWSSVFTFVTPPKKGKPHPFTVGIFGDTQNNEFNEQFQKTAEIVGRLAEVRPDLTL
                     HMGDVVNNGSVTADWLSFLRIAQPLAANAPLMLTLGNHDVENTKGPRFQHPFSSFNGL
                     FSLPGKGTDYSFDYGNVHFVCLFSGVAPVAAENGLLRYGPDSEERRWLERDLASARKS
                     RHTDWIIVFVHYPPYSFGWSNVKRWQETIAPIIDRYGVDLCLSGHRHVYERHHPLRNG
                     QPAVDGWGITYVTNGTAGGSPQGTGGSDYPTMAFTPAERMYNYAIMSVVGKSLNYEVF
                     DSAGEKTDEFELNSLR"
     gene            14676..16952
                     /locus_tag="PLU11_01480"
     CDS             14676..16952
                     /locus_tag="PLU11_01480"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPN57804.1"
                     /translation="MKSNLLKKLVILLIAGMPGCTSPRMKMADPESPVGYVNPYMGNI
                     SHLLVPAYPTIHLPNSMLRVYPERADYTGDRLRGLPLVVTSHRGSSAFNLSPYQGSDE
                     NLQPVIAFSYDQEKITPYSYAVYLDDQETEVRFGVSHQSGLYEFTFGKSDSVALIINS
                     LKGEMCWNGKAVSGHQQLQNNVKVYIYLEADLPVRSAGVLKEGMLTGSDYCANGRNAC
                     VVLKFAAGSERIRVRYGISFINEEQARKNLEREFTGFELEPLLDYGRMTWNEALGKIK
                     ISGGTYDDKTVFYTSLYRTYERPVCISEDGLYYSAFDGKVHEDEGIPFYTDDWIWDTY
                     RATHPLRILTDPGTETRIIRSFLRMAEQMEHFWMPTFPEITGDSRRMNSNHGVATVID
                     GWSKGIREFDLEKAYEACRKGIEEKTLAPWSGKPAGELDRFYREKGYFPALREGEEET
                     IPEVHSFEKRQPVAVTLGTAYDKWCLSQIARELGKNEDYDYYSEQALNYRHLFNPETR
                     FFHPRDSRGEFIEPFSYVFSGGLGARHYYGENNAWIYRWDVQHCISDLVRLMGGREAF
                     IASLDAMFSEPLGKSKFEFFAQLPDHSGNVGQFSMANEPSLHIPYLYNYAGEPWKTQK
                     RIRALLDQWFRNDLMGIPGDEDGGGMSAFVVFSQLGFYPVTPGLPVYNIGSPAFEESV
                     IRLDNGEEFKIIARNCSKENKYIQSAKLNGKEWNKPWFSHDDLTEGGLLELKMGSKAN
                     KTWGADETDAPPSGIVLP"
     gene            17057..17557
                     /locus_tag="PLU11_01485"
     CDS             17057..17557
                     /locus_tag="PLU11_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN57805.1"
                     /translation="MLSVRGKVTDQTRIGLPEASILIKGTTLGPLTCQEGNYQINVPD
                     VEKAVLVVGFIGFEQTEVAVNGRSTVDVQLAQSAIGLDERVVIVLGITRKSKSLGYAV
                     QSIDVNSMTEIRDANHTNMLAGTAAGVNMISAGGSTASTREEFRGNNSLTGNNQPLYV
                     VEGMII"
     gene            17609..17776
                     /locus_tag="PLU11_01490"
     CDS             17609..17776
                     /locus_tag="PLU11_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN57806.1"
                     /translation="MNYDNIVRNINPDDIESIEILKGGNASEPNDSDAANGVILITTK
                     KAAKSADLGVT"
     gene            17956..18104
                     /locus_tag="PLU11_01495"
                     /pseudo
     CDS             17956..18104
                     /locus_tag="PLU11_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011453049.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18271..20037)
                     /locus_tag="PLU11_01500"
     CDS             complement(18271..20037)
                     /locus_tag="PLU11_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN57807.1"
                     /translation="MISNKKTIKSKLFTCFFILLAIFAVSILVFQDHREKQFRIALME
                     NQLNEYSDLTNRYIENNNLLSTNDFGKLDSIKDLIPSAKIRITVLDLAGRVLYDNSVE
                     NYSEMENHLDRPEVQKAKYADRGYNIRHSVTTKQDYFYFAKFYKTIFIRSAVEYNAEV
                     INYLKVERAFIIYLLFLFAIFGLILRLVANRLGDSVSRLKEFTIKASRDEELNPEIQF
                     PEDELGTISREISLAYERSRKVKAELMLEKEKLISHLFVLKEGVAFFSPQKQVILTNS
                     HFIFYSNIISEESSVKADKILEMEAFSEISSFIDKTLNSSTGHSAELPRQEFTIQRNG
                     MHFQIQVIFFQDRSFEILITDNTQLVKRSIMKQQLTSNIAHELKTPIASVKGYLETVL
                     NNPDIDHVKLRYFVEKAHNQSERLTQLVNDIALLNKIEESSDLYPKEKVKVAKLISEV
                     IVSFSDKLKNKSVKFEIGMDENVTVNVNYSLLFSVFRNLTENSVDYGGDNITIQITNY
                     HEDNNFHYFSFSDNGQGVEEEHLPRIFERFYRVDSGRSRKEGGTGLGLAIVKNAILSF
                     KGEISVRNKKGGGLEFLFSIPK"
     gene            complement(20056..20742)
                     /locus_tag="PLU11_01505"
     CDS             complement(20056..20742)
                     /locus_tag="PLU11_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57808.1"
                     /translation="MVEKYRILVAEDEEDLCEILQFNLESEGYVVDAVHSAESALKKD
                     LTVYDLLLLDVMMGRMSGFSLAQKVRKELHLEVPIIFLTAKNDENEILTGFNLGADDY
                     ITKPFSVKELQARVKTVLKRLHRGKPEEEPETETVGNMLIDYVGKRLIIQNIKIDLTR
                     KEFEILVFLVRHSGRVFSRNDLLARIWENNVIVNERTVDVNIARLRKKMGECGNYIHN
                     KSGFGYYFEV"
     gene            complement(20770..21459)
                     /locus_tag="PLU11_01510"
     CDS             complement(20770..21459)
                     /locus_tag="PLU11_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPN57809.1"
                     /translation="MNIKKENAILEVIEKFSDYSELILHQLTLLEKIINSGSQLIPSD
                     ILEEIMSNEEKSDKFEIKLSEKIINTIVLQQPLASDLRKLMACYQMVINLERIGDLVI
                     NIVNFIPKIKDQEIYGIMTDVIYNMLIVTQNMVQKSILSFTQGDREYAIWTIKNDPVV
                     DEMNQKLVKKVISKTGLPEETRQILLSFIHTNSIISSIERIADHATNVAEATIYALDG
                     TDIRHQNKEEK"
     gene            complement(21496..22278)
                     /gene="pstB"
                     /locus_tag="PLU11_01515"
     CDS             complement(21496..22278)
                     /gene="pstB"
                     /locus_tag="PLU11_01515"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPN57810.1"
                     /translation="MTENKMHIEHPALSLRNLSVAYTKDKPAVKGVTADIKRHSITAI
                     MGPSGCGKSTLLRAINRMHDLYPEIVMQGEIRLNGEDVFNMSTTTLRRKIGMVFQRPN
                     PFPTMSVYANVLAGYQLNGFSLKRSEKDEIVEKSLMDVGLFDEVRDSLNKKGTFLSGG
                     QQQRLCVARALALKPEVILADEPTSALDPLSTARIEDLFTELKKNYTIVLVTHNMAQA
                     ARISDNAMFMYLGELVEYDRTKTMFTNPKDPRTEGYLTGKFG"
     gene            complement(22289..23182)
                     /gene="pstA"
                     /locus_tag="PLU11_01520"
     CDS             complement(22289..23182)
                     /gene="pstA"
                     /locus_tag="PLU11_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPN57811.1"
                     /translation="MKSDNRNTPDRSSWRDKKNKLFLGLVVTLSIVTVLPIFLILWKL
                     VKEGISQINLDFFIKVAPDPLEAMTAVNNGEIIPGGIANGITGSLLMVLIASLIAIPV
                     SILAGIFLYEHPNGWYARLIRNLTDILQGVPSIVLGLIAYIWIVISITKGFSGLAGSV
                     ALAIMMLPIVIRSTEETMKMIPVTLKEAAAALGVPYWRIILKVMIPTGFSGLMTGILL
                     SISRILGETAPLMLTALGSTMINLNIYKPTSAVPLLIWEFYNNPNMVRLVWSSSLFLM
                     GVVLVLNILAKRINSRFGNNQ"
     gene            complement(23230..24105)
                     /gene="pstC"
                     /locus_tag="PLU11_01525"
     CDS             complement(23230..24105)
                     /gene="pstC"
                     /locus_tag="PLU11_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPN57812.1"
                     /translation="MIRSREIFTSDKIAKVILKSASFLVLLLAAGIIFSLLKGSIPAF
                     REFGIFRFIFSSEWNPTEGKEVYGAFPFIFGTVVTSLLALIICFPLSFATSLFISEYF
                     KKTKIAGILSTLVDLLAGIPSIVYGLWGFYTLRPLIVDLGLSEQGYGIFTSSLILAIM
                     IIPYATSLSNEIISMVPNELKEAGYSLGATRLEVIFNVILPVSRSGLVAGYILALGRA
                     LGETMAVTMLIGNANLIPKGIFSPGNTMASVIANQFGEADGLKLSALIAIGLLLFVVT
                     AIVNAAGKWIVRRYA"
     gene            complement(24112..25086)
                     /gene="pstS"
                     /locus_tag="PLU11_01530"
     CDS             complement(24112..25086)
                     /gene="pstS"
                     /locus_tag="PLU11_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPN57813.1"
                     /translation="MRAEITAAGATFPQPFYNKVFNDYSTMTGNKLTYGGIGSGGGIR
                     SLKDKVVDFGATDAFLTDEQAAELPAPVVHVPTCLGAVVIAFNLPGITELKMKNEVLA
                     SVFLGEIKKWNDPKILADNPGINLPGKDISVVYRSDGSGTTFIFSDYLSKISKSWADK
                     VGTGKSLNWPVGMGAKGNPGVAGIIKQTEGTIGYIGSEYAFAQKIPCVLLQNQAGNFI
                     APSVESVSAAAQGDIPADTRVMVTNSADPDAYPISAFTWIILYKEQSYNGRKIDQARE
                     TVKFLQWLVGPDAQAIARKVDYAPLPEKAVQLANDILKSVTFEGEPLL"
BASE COUNT         7126 a         5741 c         6079 g         7081 t
ORIGIN      
        1 ctggcgccag cgtaactctt agggatggga agcaacggca tgggagcggt gatgataggg
       61 ctctcattat cctgggttct gaccttcaga acatatttgt ggcggatgat cctgcgcaca
      121 aaccaacggg gaggttcctt ctccagcaac tcggtgactt caggttcaag ttcgataaaa
      181 gcttcgacgc atgtcagcgg gataagaata catgtagtat ctcatcgaag aggtaaggct
      241 gaacatgcta ctgttgtgtt gaagccaaca gggttcttac catctccaga tccgaaagac
      301 cgttgacctt taccctgatc ccattagggt aattgatctc acaagtcagg ctcatgacgg
      361 aagaagcatg tttgttatac tgttccggtt ggttattcat ggctaccgga agttccgggg
      421 atggtttttg ctgattactg ctgaaaatga caggtacgaa tcctcgttta ggaggcaaca
      481 cacctttcag tttgttctgc caataaaaga acttgctttc atggatgctc tggtttgtac
      541 agaagtcccg gatagttaaa ccggattcca gatagttact gtaaatggcc cggaatgctt
      601 tttcgttcat catgtcggtt gctttatgtc aaaaagcaaa cataaatccg gatccgatta
      661 aaaaatgacg tggttcaccg aatgcttaca aaagttgttg atgaactcaa gaacatatgc
      721 cagatcaaac acacccgcca cagaagccag gggggttatt actaacctca tatcagggtt
      781 gatcggttat gggcacttgc cgaaaaagcc ttcccttaac taggaaatcg ttgaccctaa
      841 ggctttcaat tcgaaatgtc atgtcgaact cacgttagtt tttacccaaa aggttttgta
      901 aataaaagtc cataaaaata caatacaaag taaagtgggt tatttttcta taaaatattg
      961 taaattagaa atttaatatt tccaacaatg ttttggggta tggtatgtta taaagatttt
     1021 tataatcgcg ggtaaattta tcgaatgctt ctttggctag atgatgtttt ccctggttag
     1081 ttagggtttt gcatttcaac agaatggctt cctcattcag catgtcatac aataagaccg
     1141 tatgggcaag atgaagcgcg aggttcggat ctcggctggt atccatctga tcgctgtatc
     1201 tgatcagggt atcaatgata atattagaga gttcagcctt gaattcgtcg agccagtcaa
     1261 aagaaagact ttccagaaaa tggcctttct gggagattct gatcagttcg cagatatcat
     1321 ctctccccgg ttgaccgtaa tggcgtgcag tgcggaggca gtgccaatag tcgttataaa
     1381 cggtttcttc attaaaaatg ctcttccagt agcctgtact cttcgataac tctatcgaat
     1441 ccagttcact taagagcgtt ttaagcttaa caatgttcac cgcaagattg ttcctggcgc
     1501 ttttttcatc cttatccaac caaaggattt caataattct ttccgctgaa atgcctttat
     1561 cttttatgga gttaagccag atcagcagaa acagctcctt gagcaaaggg gaaaacttgc
     1621 tggtaatgtc agagccggta cggttgaaga tctgaaaatc acccaagaac aggatggtat
     1681 ttgatttggg atgaatgttg gtaaagagat cagcggcaga ggaaatatct tggatttcag
     1741 atagtttctg gtccgttgaa ctttcctggc tgacggtact tctccgtgtt cccatctttt
     1801 tttcaactgc cagaaaaagt aaccatgaaa ccaggaacaa ggcggggaaa agaaaaagca
     1861 gatttttgtt ccatccggag gttttttcta tgtcagtaag ggaccccgga gggtatccga
     1921 tggtgtacac ctgtgcctgt gttttgttgt tctccgtttt gagcagggtt acgcaaacca
     1981 gtttccagct tgtctggcaa agaaaaaggt cggaaaagga ttggatgtcg tgaaacagat
     2041 acggaatttt atctcccgcc agggttaatt cgggtgaatt gagtctcccc ttcattaact
     2101 gtaggtaacc ttcatacctg aaaatgggaa atgccagaac atagaattct tgatggtcag
     2161 gatcaatcac catggagttt gcaaaggcga tgtcctccat cgggggatca aattctcgga
     2221 ttaagctgaa atgattatgt tgaatgctgt aggcaacgag gtccttgtat gttcctggat
     2281 tcagaatttg tttaccggta aggcttccgt aacctccaag gatgaatacg gtgtcgttat
     2341 tcatgcctga tgctgaaaga taacggggat ggaacagctt atcactggtg atgagggtat
     2401 cccaccggtt ttcattaaac ctgaatcgca gtacgctgtt tttatattca tgttgtccgt
     2461 aacctccgaa aatgtacaag gtactgtcct tttcctgaaa atatttgttg tggtgccaga
     2521 aaacggtgag agggtatgcc ggtgaggccg tttgttccca taaccgggtt gtcaggttga
     2581 acctggctat gcttttcaaa tctacattat agcttacaac ggagcgatgt acaggatcat
     2641 aaaaggcttg acagccctgt tttaaaacgt cgggtttatt cagatacctg accgtatcaa
     2701 gcctgttttc cttaacggaa tagaccatta acaattcgtc ccctatgatg tataccacct
     2761 catctttctg gttaaaagcc acttctccgt tgccctcaag caaaatatcg gcgtttttgg
     2821 cccagaattg aaatttaggt ttcagccaca cgggattcat agccaaggca ttgacttttc
     2881 ccttggaatc aaaggcaata ttcccggcgc tttcgtttaa cttccattcg tgaatcagtt
     2941 gtccttcatg ccgaagcgtt atgtcacgga tattcatggg agggacatcg gtagtcttga
     3001 agttctcaaa atcagacgca ccgaaaagta ttttaacctt ctggctgaat ttaaccggat
     3061 aagcggctaa ggttgtgtca ggcaatacca gaaggaggga ttgttccctg tagttgtacc
     3121 ttaattcaat gggaatccac tctctggata gttgctcaaa atcggacctg gctgaaatgc
     3181 tcgtgacttc ctcgccatag accagatcaa aagtggagta agaagtgcgg taattataaa
     3241 tcagatccac attttcattc tcactgtcga taattctgac gacataccca aaaacggctg
     3301 cttcgtgcga acgcatggtc attgcgaagg agagggtaaa attgtcgtga acattaaaga
     3361 aatggttggg agaaagatcc agatgggtcc tgttttcctt taaatcagag tggcttgaaa
     3421 actgcaggcc gtaaagctgc cctgacaagt ccgcaggaag aaaaaaagag aggataatca
     3481 aataatagag gattctatta tgaaaaggga ctgaagtata gaatgatcgt acaaaagtgc
     3541 tcatccaaaa ccttattttc gaattccctg gatatcaaac aaaaacatta aaaggataaa
     3601 tattcttaga tgcatcattt catgccatca tcatcgtgct aaattacaag acaaattaaa
     3661 actttacaac tcttttaagc ccgtagtact ttttttacgc tttcgctcct gattaatgct
     3721 gatttcttat tcggttaatc tattcttaat cggaaattaa cctttcccgg ttaactttga
     3781 agtaatcaat tcaaaaacca tgaaccagaa caagatgctg ttacggtttc tcctcttttg
     3841 ttttccatgg atgtttatca gcgggtgcgt tgcttacaat tccggagata aaggagaaat
     3901 tcccgtaagg gaaaaatcag cgagttacaa aggattgatt atggccggct accagggttg
     3961 gttcaatgcc gatggtgacg gggctgggag aggctggaac cactattgga aaaaccagcg
     4021 tcttgcaccg gggtcttgca aatttgatta ttggcccgaa atgtcggaat accctgttca
     4081 atacaactct cccttttatt tttctgacgg gaaacaggcc acccttttca gttcctatga
     4141 tgagagtaca gtagatctcc atttcaaatg gatgaaagaa tatggtatcg acggcgtatt
     4201 cattcagcgg tttgtcacca atttaaacaa cccggtcagc tatgggcaca accagaaagt
     4261 actcgcgtcg gcgtttaagg ctgcggaacg gcatggcagg attctttgca tcatgtatga
     4321 tctttcgggg attgccgccg gtgaagagac cctgctgatg gaagactgga agaaactggt
     4381 ggagcaatat caacttaaaa atgagaatcg attagataat tatctctgtc acaatggtaa
     4441 gcctttggtg gtcgtttggg gggcgggatt taacgataat cgcaaatacg gactgaaaga
     4501 ggtgagaacc atcgttgatt tcttaaagaa cgattcgtta tacggtggtt tctcggtcat
     4561 gctgggagtg cccacctatt ggagaacgct gaaaaatgac acgcagtccg atacgctgct
     4621 tcataccatt gtccgatcag ccgatattgt gcagccttgg tttgtcggtc ggtttaatga
     4681 agagacttat ctaggcttta aagatttgtt gagagacgat atggcctggt gcaaggagaa
     4741 cggtccggac tatgttccag tggtttttcc gggcttcagt tggcacaata tgtatccaaa
     4801 ttacccatct aatcagatac ccaggaacaa ggggcaattt tactggaagc agttggcagg
     4861 agttctttcc ttgggagctg aaatggtcta tgtggccatg tttgatgaga ttgacgaggg
     4921 aactgccatt ttcaaatgtg ctcaccaggt tcctgttgga gaaagcgtat ttgttccttt
     4981 ggatgaggag attccctcag accattacct gtggctcacc ggccaggcgg gccggatgct
     5041 ccgaaaggaa atcccagtat cggcagaaat gcccatgaaa acccataact gaaatattta
     5101 aattctatga gacaaacttt gattatgatt tcactgttac tgatgtggtg tgtcccggtc
     5161 agggcacaag tactgtcagt gcagggaaag gtaactgacc agaacaatgc gggtctgccg
     5221 ggggccagca ttgtggttaa ggggacaaca gttgggacga ttaccgacat caacggtaac
     5281 tacctgctga atgtccccaa tccggagcaa gctgttttgg tgtttgcctt tatcgggtat
     5341 gacatgcttg aaataccggt caggggccag tcaaccatca atgcggtgct gagcaccagc
     5401 aatatcggac tggaagaggt ggtagttacc gccatgggga ttacccgcga tgctaaagcg
     5461 ttgagttttg cccgtcagag tgtcagtgtg gaagagatga cagaagccag gagccccaat
     5521 atcgtcaact ccttctcagg gaagattgcc ggggtgcagg tcgttccttc cgggtttaat
     5581 acgggctctt ccaggattgt gattcggggg aacagctccc tgaccgggaa taaccagccc
     5641 ctgtttgtcg tcgatggagt tcccatcgac aactcgcccg gcgattccgg tagcatcgac
     5701 tacgggaaca atgcggctga cctcaactcc gacgatattg aaagcattga agtgctgaaa
     5761 ggtcccaatg cttcggcctt gtacggttcc agggcggcca acggggtgat cctgatctcc
     5821 actaaaaaag gtacccccaa gtttaaaatc accctgaatt cggggaccat gttccagacg
     5881 ttgactcaat tccccgaata ccagaatgca tatggggtgg gtacctcttt ttacatcgac
     5941 aatcagcata tgctgccttg ggccaacgta aattacagga gttggggttc ccccatgctg
     6001 ggccagcctt acgtggcctt gaacggggaa gtgaaacctt atctgccgca acccgataat
     6061 gtgaagaact tttatcagaa ggcccgcctg ttgaccaatt cggtggccct ggaaggggga
     6121 aatgctgata atattgtcag gttttcctat accaactata atgggaacag cgtggtggaa
     6181 ggattcaata aaaacaccaa gcacaacgtc aaccttaggg tccagaataa attcaggaag
     6241 tggatcactc ttgataccaa ggtgaatttt atccgtgaca atgtggtcaa ccggcagtat
     6301 tcgaatgaca acgggcgcaa cccgtcatac atgtacgccc atatggcccg gagcactaac
     6361 ctggatgaac tgatcccctg gaaagatgaa ctgacaggaa aggagatagg gacccaccgt
     6421 aacttcagca atccctattg ggtcatccac gaaaatccga acgaagacac caaggacagg
     6481 gtaatcgcca cctttaatcc tgaaataaaa atcacggatt ggatcaaact tatcggcaga
     6541 ttcggcgctg atatgttctg gaccaatggt tatgaattca acaacatcgg atccattatc
     6601 gccagcaatc ccaacggttt tcttcggacc ttcaatgtga accaacagaa tattaacctg
     6661 gaaggcattc tttctgttaa taaaaagctg gaggcatttt ccgtttccgc caattttggc
     6721 ctcactcggt ttgattcccg gtctgaacgg agggattcga gaatcaactc cttgctgcaa
     6781 cccggcctga tcaatctgtc caatgccaag gaataccctg ttgtcagcca gaaccagagc
     6841 cggaaaagga ttaattccgc atttggtgcg ctctcttttg gttataagga ttttgccttt
     6901 gtcgacctga cgggccgtaa tgactggtcc tccacgcttc cgtctaaaaa caactcctat
     6961 ttttatcctt ccatcggggg gacgctgatt attaccgagt tgctgaaagc aaagagcaat
     7021 ttgctcagtt ttgcaaaact gagagcctcc tatgcgattg tcggtaatga ttccgatccc
     7081 taccgactcg atcagacctt ttcctttaac ggatttttca acgacgcgac agtggcctct
     7141 ctgagtacga ccatgaacaa tccagacctg aaacccgaga aaaccacctc ctatgaatac
     7201 ggcttggatc tgcggttttt caaaaacagg ctggcgctgg atgcgactta ctatgatgcc
     7261 tccaccgtca accaaatcat taccgcctcc ctgccgacat ccagcggttt tgagcgcaga
     7321 ttgtacaatg ccggtgaaat caaaaactgg ggatacgaac tgagtctgac cggacaacca
     7381 gttcaacgag ggaatttcaa atgggagacc aggattaatt tttcaaagaa caactcagaa
     7441 gtggtgtccc ttctcgacga cattccccgt ttccagcttg ccaaccgatc gagttactta
     7501 tatgtgtacg cggaggtggg taaaccgtat gcttatctgc gcgggctggg agtcaaaagg
     7561 gataaccagg gaaggatgtt gctcgaagac ggaggcggtc tgctccagaa aaatacagat
     7621 atgccctttg gaaccgccag ccccgactgg cttgccggaa tcggaaatac cttctcatgg
     7681 aagggattgg atctctattt cctggttgac atcaaacaag gcggggtcat gtattcggga
     7741 accatctcca gaatgctgac caacggtatg tttgccgaaa ccctcgaggg tcgcgatgac
     7801 ttttacctcc acagcgtcat ttggggtgaa aacacttcgg aattgaccgg aggtgctaga
     7861 tgggacgctt actttaatga cggcacaaaa aacacaaaat tcatgactcc ccaaaattat
     7921 gaatatgccc ggcccaattt cgccgaattc gttatttatg atgcctcgtt cgtgaaactc
     7981 agggaagtat ccttcggata ttatttccct aagctgctgt tggccaaaac tccttttgag
     8041 aatgccagga tttcagccac cggaagaaac ctttggatcc tgcattccaa tactccgcag
     8101 ggcattgacc cggaagcctc ttctacctcc ggaaatggac agggcatcga aaacggagca
     8161 ttacctccca acgccatcta cggaatcaat gtaagactca tcttttgaaa atgacgatca
     8221 tgaaatcaaa acttatctat atagcgataa tggtgggatt gttctcttgc actgatcaat
     8281 tcgaagaaat taatcagaat cccaataatc cggtaaaagt agagcctgaa ttccttctga
     8341 ccacttccct gagagaaacc cagaatcttt acggaggcaa catgaacggc gttgtgtttt
     8401 ataactacac ccactatttc tcgggatttc agggggagtt ccagcgatat acctatagtg
     8461 tctcctccac caatgactac tggcggaaca attatgtcag atgtttgcag ccggtgcatc
     8521 agatcgtgaa gctgtatgaa gaggatcctt cctataagaa cagggtgatc atcgccaaga
     8581 tctggaaggc ttatattttc tccaatatcg tttcagtctg ggggggtgtt cccatgagcg
     8641 cagcgttgga aggcacaccg ggggttgcct atgaaaagga acaggatata tacactgcct
     8701 tgctggatca gttgaaggag cttgccgaag cgattgatct taccggggac acatacaaag
     8761 ccaatgctga taagatctat ggcggggatt tgatgaagtg gaagaaattt gcccaaacgc
     8821 tccgcttgcg actagcaatg cgcatttccg ctgccgatcc agagaaggcc aaaaaaatcg
     8881 cactcgaaat ttcccggaac gcaacaggaa ttatctcgtc gaaagcagaa acggcagcaa
     8941 tgaactgggg gatcaccagt gactcttgga gttaccttta caaccgggtg gtgtacaact
     9001 atacggccaa taaagctacc attcctgtgc tttgtgaatc actggtctat cacacgctgc
     9061 cctataacga tgccaggctg cccgtatatg gtcagccagc caaacaaggg ccttccatcg
     9121 gcaagtattt tggccagaac atctcctatg gtggtggtgg cggctatacc acccgcacca
     9181 acccccattc cctgctcaaa caggatgact actcctatat cggggagcgt ttccttcagc
     9241 ctgatgcgga atatgtcttt ctgtcctatg cggaagcctg tttcctgaaa gctgaggctg
     9301 ccctgaaagg atggtggaag gatacgaatg cccagaatta ttattatgag ggaattgatg
     9361 cctcctatga ccattatgga ttatcggcag atcaggctac cgcctataaa gagactccgg
     9421 gaatcaagtg gggtacagag accgattcaa cgggtcgttt ctatgaattt caggactgga
     9481 tgggcatttg ttccagttac atcaaggcaa acgattttaa ccggcagatc attatgcagc
     9541 actggctggc catgcctaac caaggcgtgg atgcctgggc gctgatccgg cgcacgagaa
     9601 tccttgaatt ccagccccaa ttcgctactt atgacggcga atatgcctat attcctgacc
     9661 gcattccata tcccggaagc gagtattcca ccaacccgtt ggaggtcgaa aaggcagtct
     9721 cttggctggg aggccccgat ggcctgttca ccaaactatg gtttggcctg cccaatgtga
     9781 aaaatccaaa tttaccccat taaggaatga ctttgaaaat caaaaaatga taaatatgaa
     9841 aaatatattc tatgccctgg ttggtctgtt cctttgggtc tcgtgtgaag agacagacga
     9901 acacctgccg ccgcagttca actatgagat accgccgaca gaattgtcag acgatgtgct
     9961 ggtaggagcc ctctattata attatgcgac tgccgactgg gcaaagaagt acaccaacac
    10021 cccacaactg ggtcagtaca gcgccttgtc ggccgaaacc atgaagcagc accgcatttg
    10081 ggccgatcag gccaaaatca actttttcat tttcccgtgg aacggcactt ccggtaatgc
    10141 cctgctgacc agcttcatta caggaagaag cgaaaatgtg aaaatggtgt tgaactacag
    10201 tacagcccat ttatcggcga ccaatgcctc tccgctggcc ggcgccaagc tgactaccat
    10261 ggtgaatgag tttaagtccc attatatcac tcattttgac aaggagtatt actttaagat
    10321 cgacggaaag ccggttgtca tcattagtcc cgttaatctg gcaagttccg ctgcggcaag
    10381 cattgattat ccgatggtca tcacggcttt acgaaccgag ttgaagaacg acgggaccga
    10441 cctgttcctt attggtgaaa taaccagtgg ttggttgccc ccacagcggt atgccactgc
    10501 cctgaaaacc tttgacgccg tcgtgttgag caactggacg gccaacggta attacggcta
    10561 tgacaggtct gtattctatc ccgccttttc cgatcaggcc tttaaaaact gggccgattc
    10621 cacttcggtg tggggaatcg actttgttcc ttgcatcatg cccgggtttg acgataaggt
    10681 aatgacgccg gccagcaaga attttaatat tgttagaagt gcaaaactct ataccgacat
    10741 gtgcaatgtg gctaagagaa acctcggcga aaagaaaatc accattatca attcgtggaa
    10801 taattttcag tttggcacct ccatcgaacc tacctccgag tacggtaccg aatacctcga
    10861 aataaccagc aagcaattta aagttcagtg attaagtcac atggatgttg cttgaatatc
    10921 actttattaa ccatatttcc gcaaataact taatatgaaa cgattcataa ttactattat
    10981 catcgttctc tttatatttt cggaggccct gccccagaag agcagtatga ttgttttggg
    11041 ggatcttcat tatgatctgc tggaagatca tgacatggaa tggctcagga caaaacctga
    11101 cgatctcagg caggtcacga aggaatatac ggtttacacc gacaaatact ggaacgattt
    11161 catggcggta cttttgcaga aggctgaggc cgtcaggcct cccctgaggg cggttgtcca
    11221 gttgggggat ctctcggaag ggctggcagg ctccccggag aaggcgaagc aaatggcggg
    11281 caacaccatg aaggccatcg aaaaggtggg tatggaggtt ccctggattc tggccaaagg
    11341 gaatcatgac ataaccggac ctggtgcacc cgaagccttt caggagtttt atgtgcctgt
    11401 gatcagggaa cagaccggaa atcctgaaat tcaaaatgcc agctacactt atagctttga
    11461 caatgtatct gtcacctgta ttgatccctg ggaaagaggt ttcagcatgg tggatttcct
    11521 ggatcaggaa ctgaccaaat cggcagctct ggtcaaattt gttgcgatac atgaaccggt
    11581 aatcccggtg accgaaaggt gctggcatac tatgcgaagg aatccggagc agcgcgtaaa
    11641 actccttgaa gtcatcgcac ggcataaggc cattgtgctc tgcgcccatc tgcaccgcta
    11701 ttccgtggta aagagaaaca ccccctgggg acccatcgtc caggtcatgg tcatcagcgt
    11761 cattaaggac aaggattacc agaaacctgc aaaggtgatc acccaatatg gaccctccct
    11821 ggcggaagct ttccccgact ggcagcctga aaccctggaa gcacggaagg ctatgctggc
    11881 tgaagaagcg aaatacgtga ccttttacaa acaaaccgac ttgccgggat atgcggtatt
    11941 caaaatcaac gccaaaaaga gaaaggtcga cttggaatat tatgccgcct tcggagccaa
    12001 accatatgat aaacttgcca tttcgaacct gatgaaataa caagtcacga tcatgcacca
    12061 accgggatca tgatccacgg gcaagtatgg gttccataaa attgaccgtt ggagctcaat
    12121 atattgtatg attgaaactt gagtgaagca attattacgt gacttaaaga ttgccggtga
    12181 tgtttttttt caaacggaaa tttttcacgc tccttttggg gttcatagtt acctgcggcc
    12241 aatcccagga tataagtttc atagcactgg gggatttgca ttacgaccgc ctcgacctgc
    12301 atgactggga ttatgtgatg aaaaggcctc aggattttga acagataatg aaggagtatc
    12361 cgcagattac cgcggtatat cttcccaaat tcctccggct aatcaaagag cagtccttgg
    12421 tgattacccc tccggttaaa gcggtggttc aactagggga tttggtggaa ggggttgccg
    12481 ggaatgaaag gcttgcccgg gaaatgaacc gcggcgttgt ggaccttctg tattccgttg
    12541 aaatggcggt cccctggatt cttgtcaagg ggaaccacga cgtcagcaac agtccggggc
    12601 aaccagaagc ctggcaggag gtgattcgtc cctttatcga agggcaggta aacagcccgg
    12661 tgggcagggg catgtataca catcggctca ccggccaggt ggaacttttt gttttggacc
    12721 agtttttcag cgtggaccaa aacctgcccg aaaccgccat gttgtccttt ctggaagagg
    12781 tgttcagtaa atcggaggcc agattcaaat tcgtgctgac ccatcagccg gtcattcccg
    12841 ttaccgaccg ttgctggcat ctgatgagtg gcatccggcg acccctttcc gaccccaggt
    12901 taagagagcg gttgctcaat ctgctggcaa aaaataaggc catagtcttg tgcgcccatc
    12961 tgcataaata ttcggtggta agccgtaaaa ctgaggccgg tgccgtggtt caggtgatga
    13021 taaacagcgt ggtgggaagt tttgatctca aggcgtcggt ggaggtggaa acggaataca
    13081 aaggggccgg atttgttgag gaaaatccaa ccttccagtt gcataatcag gaagtgagga
    13141 aaaagatcct ggaaaatgaa aagcccaata tccaatattt tacaagagcg gatctgtcgg
    13201 ggtatgcgat catttcagtt tacgaaaagg aaaacaaggt tgttttaaaa tactataacg
    13261 gtttttccgc tgaccccttc cagtccttgg agctgtcggc atggtgggaa tagagcgccg
    13321 gccacgcccc gttccttcaa taccaaagaa ctgaaataga ccacaagagt gctaaagata
    13381 tgagagaaaa cagtgacact tctggagcag gtgggccgtt tcctttttgg atgaggagaa
    13441 ggttcctttt ggtgctattc tggacggtat tgtcatgcat cccgtcttgg gggcagcctg
    13501 aaggtgttca cctgagcctt tctgcaaccg gcaaagggga aaaacagttc atgaccgtcc
    13561 tatggtactc cagggaagcc ggcgccgttc aattcatccg gtatggcctg acagaaaagg
    13621 aactgcctaa aatggtaaag gctgattctt atggaatgga aggatcgttt ttccataagg
    13681 gagaaatgac aaaactcact tcttcaaccc gctattacta tcaatgtggt tctgacaaat
    13741 cgggttggag ctccgtcttt acctttgtca ctcctccgaa aaagggaaaa ccccacccct
    13801 ttacagtcgg aatatttggc gacacccaga acaatgaatt caacgaacag tttcagaaga
    13861 ccgctgagat tgtcgggcgg cttgcggaag taagacctga tcttacgctt cacatggggg
    13921 atgtggtcaa taacggatcg gtgaccgctg attggctttc ctttctcagg atcgctcagc
    13981 ccctggctgc caacgccccc ctcatgctga ctctgggaaa tcatgatgtt gagaatacca
    14041 aaggcccgcg atttcaacat cccttttcct cctttaatgg attattttcc cttcccggga
    14101 agggtaccga ttattccttc gattatggca atgttcattt tgtctgtctt ttttcagggg
    14161 tagcccccgt tgctgcagaa aacggattgc tgcgttacgg acctgattcc gaagaacgga
    14221 ggtggctgga gcgggacctg gcctcggccc ggaaaagccg ccatacagac tggatcatcg
    14281 tattcgttca ctacccgccc tattccttcg gttggagtaa tgtcaaaagg tggcaggaga
    14341 ctatcgcccc tattattgac cggtatgggg tggacctttg tctctccggc caccgccatg
    14401 tctatgaaag gcatcatccg ctcagaaacg gtcagcctgc agtcgatggt tggggaatca
    14461 cctatgttac caacggaact gccggaggat cgccacaggg aaccggtggc agtgattatc
    14521 cgacgatggc ttttacccca gcggagagaa tgtataatta tgcaataatg tcagtagtcg
    14581 gaaaatcact gaactacgaa gtctttgatt ccgcaggaga aaaaacggat gaatttgaac
    14641 tgaacagcct ccgttaactt aattttgtga agattatgaa atcaaacctt ttaaaaaaac
    14701 tggtcatttt gctcattgcg ggaatgccgg gatgcacctc gccccggatg aagatggcag
    14761 atcccgagag tcccgtcgga tatgtcaatc cttatatggg caacatcagc caccttttgg
    14821 tgccggccta tccgacaatt cacctgccca acagcatgct gagggtgtat cctgagcgtg
    14881 ctgattatac cggtgacagg ctccgggggc tgcctttggt agtcaccagc caccggggaa
    14941 gttccgcctt taatttaagt ccgtaccagg gaagcgatga aaatcttcaa ccggtcatcg
    15001 ccttcagcta cgaccaggag aagattaccc cctattccta tgcggtatat cttgacgacc
    15061 aggagacgga agtcaggttc ggagtaagtc accagtccgg cctttatgaa ttcactttcg
    15121 ggaagagcga ttccgtggcg ctcatcatta attccttaaa gggggagatg tgttggaacg
    15181 ggaaagcggt ttccgggcat cagcagttgc agaataatgt caaagtctat atttatctgg
    15241 aggcggacct gcctgtgcgg tctgcgggag ttctgaaaga gggtatgttg actgggagtg
    15301 attattgtgc caatggaagg aatgcctgtg tggtgcttaa gtttgcggca ggcagtgaac
    15361 gtatcagggt caggtacggc atctccttta tcaacgaaga acaggctagg aaaaacctcg
    15421 aaagggagtt caccggtttt gagctggaac ccctgctgga ttacggccgg atgacttgga
    15481 acgaggcatt ggggaaaatc aaaatttccg ggggtaccta cgatgacaaa accgtctttt
    15541 acacctccct ttaccggact tatgaacggc cggtttgcat ttccgaagat ggtctctatt
    15601 acagtgcttt tgacggaaag gtccacgagg atgaaggaat acccttttat accgacgact
    15661 ggatctggga tacctacagg gctacgcatc cccttaggat tctgacggat cccggtacgg
    15721 agacccgtat tatccgttcc tttctcagga tggccgaaca aatggagcat ttctggatgc
    15781 ccacctttcc agagataacg ggcgatagcc gcaggatgaa ctctaaccat ggcgtggcga
    15841 cggtcattga tggctggtca aaggggatca gggaattcga tctggagaaa gcctatgagg
    15901 cctgccgcaa aggcattgag gaaaagacgc tggcaccctg gtcaggaaaa cctgccgggg
    15961 aacttgaccg gttttaccgg gaaaaaggct acttcccggc attaagggaa ggtgaagagg
    16021 aaacgattcc ggaggtccat tcctttgaga aacgccagcc tgtagccgtc acccttggaa
    16081 cggcatacga taaatggtgt ttgtcgcaaa tagcccggga actaggaaaa aatgaggatt
    16141 atgactatta ttctgagcag gccttgaatt accgtcatct tttcaatccg gagacccggt
    16201 ttttccatcc cagggacagc cggggggaat tcatcgagcc tttcagttat gtattttcgg
    16261 gtggactggg ggcgcgacac tattatggcg agaacaatgc ctggatatac cggtgggatg
    16321 tgcagcattg catctccgac ctggtcaggc tgatgggcgg aagggaagct tttattgcca
    16381 gccttgacgc catgttttcc gagccgctgg gaaagagtaa gtttgagttc tttgctcagt
    16441 tgcctgacca ttcgggaaac gtggggcagt tctccatggc caacgaaccc agcctccaca
    16501 taccttactt gtataattac gccggtgaac cctggaaaac acagaaacgg atacgcgcct
    16561 tgcttgacca atggttccgc aacgacctga tgggcattcc gggtgatgag gacggcggtg
    16621 gcatgtcggc atttgtggta ttttcccagc tgggttttta tccggtcacg ccagggcttc
    16681 ctgtttataa catcggtagt ccggcatttg aggagagcgt gatcaggttg gacaatggtg
    16741 aagaatttaa aattattgcc cggaattgca gcaaggaaaa caaatacatt cagtcggcaa
    16801 aattgaacgg aaaggagtgg aataaaccct ggttcagcca cgatgacttg acagagggtg
    16861 gtcttctgga actgaagatg ggaagcaaag caaacaaaac ctggggtgct gacgaaaccg
    16921 atgcccctcc ctcgggaatc gttttgcctt aaaacataaa tcacctaatc attaattata
    16981 tcaaacaata cttattagac gatttttaat tctcatctcc ctgaccctga ctggttgtgc
    17041 attccttacg gcacaggtgt tgtcagtcag ggggaaggta accgaccaga ccagaattgg
    17101 gttgccggaa gcaagcattt tgatcaaagg gactacgctg ggtcctctta cctgccagga
    17161 gggtaattat cagatcaatg tccctgatgt ggaaaaggcg gttctagttg tcggatttat
    17221 aggttttgag caaacggaag ttgcggtgaa tgggaggtcc acagtggatg ttcaactggc
    17281 ccagagcgcc ataggcctgg atgaaagggt ggtcatcgtc ctggggatta ccaggaagtc
    17341 gaaatcgctg ggttacgctg tgcagtcgat agatgtcaat tccatgactg agatacggga
    17401 cgctaaccat accaacatgc tggcaggtac ggccgcaggg gttaacatga tctcagctgg
    17461 cggatccacg gcatctacca gggaggaatt caggggaaac aactccctga ccgggaataa
    17521 ccagccgttg tatgtggtcg aagggatgat catttagaac gatatgggga agtggacggc
    17581 cagcctgtga gtgccaaagg aataaccgat gaactacgat aatattgtca ggaacatcaa
    17641 tcccgatgac atcgagagca ttgagattct gaaaggaggc aatgcctctg agccgaatga
    17701 ttccgatgcg gccaacgggg tcatcctgat caccaccaag aaagccgcaa agagcgctga
    17761 cctgggtgtc acctaatttg agttcggtat aagaatgtta tttaacgtat tagttatcag
    17821 tattttgctg ataggtacat ggaaatcagg tatataacaa aaaggatttt tcctgaatta
    17881 gtttcgttaa tgaacaagga agaatccatg aaaaacagca ttatgtttct ttctgataaa
    17941 atttatcttt gaaaacgtat tctgattgaa aaagttgtcg atgaacttaa gaacatatgc
    18001 cagatcaaac acacccgcca cagaagccag gggggtttat tactaacctc atatcagggt
    18061 tgatcggtta tgggcacttg ccgaaaaagc cttcccttaa ctaggacatc gttgaccctt
    18121 aggctttcaa ttcgaaatgt catgtcgaac tcacgttata tgttcttgat ggtagcagtt
    18181 taaaccggca agtagcagtg tttcaaaccg gcgccgaaat tttttctggg aattacagaa
    18241 aaatatctgt aatatcctca gttaaaagta ttatttaggt atggagaaca ggaattcaag
    18301 tccgcctcct tttttattac gaactgaaat ttccccttta aagcttagaa tagcattttt
    18361 gacgatagcc aaacctaagc cggtaccgcc ttcttttctg gaacgccccg aatccacccg
    18421 gtaaaaccgt tcaaaaattc tgggaagatg ctcttcctcc actccctggc cattgtcaga
    18481 aaaggaaaaa taatgaaaat tattgtcctc gtgataattg gtgatttgta tagtaatgtt
    18541 atcgcctccg taatcgaccg agttttctgt cagattccgg aagaccgaaa ataacaggga
    18601 gtaatttaca ttcactgtaa cattttcatc cattccgatt tcaaatttta cagatttgtt
    18661 tttcagtttg tcactgaaac ttactattac ttcgctgatg agttttgcca ctttaacctt
    18721 ttccttcgga tagagatcgg aggactcttc aatcttgttc agcagggcaa tgtcatttac
    18781 cagttgggta agacgctccg attgattgtg ggctttttcc acaaaatatc taagcttgac
    18841 atgatcaata tccggattgt taaggaccgt ttcgaggtaa cctttaacag atgcgattgg
    18901 ggtttttaac tcatgtgcaa tatttgaggt caactgctgt ttcataatac tcctttttac
    18961 cagctgtgta ttatcggtaa taaggatttc aaaactacgg tcctggaaaa atatcacctg
    19021 gatttgaaaa tgcataccat tacgctgtat ggtaaattcc tgacgtggta attccgcaga
    19081 atgacctgtg gatgaattga gtgttttgtc tatgaaagaa gatatttcgc tgaatgcttc
    19141 catttctaaa atcttatctg ccttgacaga cgattcttca gatataatgt ttgaataaaa
    19201 gatgaaatgg ctgtttgtaa gtattacctg tttttgaggg gagaaaaaag ccacaccttc
    19261 tttaagtaca aaaaggtgac tgatcagttt ttctttttcc agcattaatt ctgccttgac
    19321 ttttctggat cgctcataag ccaatgaaat ttctctgctt attgttccca gttcatcttc
    19381 cgggaattga atttccgggt ttaattcctc atcacggctg gctttgattg taaattcttt
    19441 caatcttgaa acggagtctc ctaaacgatt tgccactaat cttaaaatca gaccaaaaat
    19501 ggcaaataaa aacaacaggt agatgataaa tgcacgttca actttcaggt aattaatcac
    19561 ttcagcattg tattctaccg cagaccttat aaaaatagtt ttataaaatt tagcaaaata
    19621 aaaatagtcc tgtttggtgg tgactgagtg acggatattg tatccacgat cagcatattt
    19681 ggctttttgc acctccggcc ggtccagatg attctccatt tcggagtagt tttccacgga
    19741 attatcatat aaaacacgtc cggccagatc taaaacagtt atacgaattt tggccgatgg
    19801 aatcaggtct tttatggaat caagtttgcc gaagtcattg gtggaaagca ggttgttatt
    19861 ttcgatgtat ctgtttgtca gatcactata ttcattaagc tggttttcca tcaaagcgat
    19921 acggaactgc ttttcccggt gatcctgaaa aacaagaatg gataccgcga aaatggctaa
    19981 cagaataaag aagcaagtaa acagtttgga ttttattgtt tttttattgc tgatcatctt
    20041 tcttagttca gaaatttata cttcaaaata atacccaaat ccggatttat tatggatata
    20101 gttaccgcat tcccccattt tttttcgcag tcgtgcgatg tttacatcca ctgtccgctc
    20161 attaacaatc acgttgtttt cccatatacg ggccagcaga tcatttctgg aaaaaactct
    20221 tccggagtgt ctgacgagaa acaccaggat ttcaaattct ttccgagtaa ggtcaatttt
    20281 gatattctga ataataagcc tcttccccac atagtctatt aacatatttc ccactgtttc
    20341 cgtttcaggt tcttcttcag gcttgccccg gtgcaatctt ttgagaactg ttttaaccct
    20401 tgcctgtaat tctttgacgg aaaagggttt ggtgatataa tcatccgcac caagattaaa
    20461 tccggtcagt atttcatttt catcattttt tgccgtaaga aatatgatgg gaacttcaag
    20521 atgaagttct tttctgactt tctgtgccag actgaaacct gacattctac ccatcatgac
    20581 atcaagcaac agcaaatcat aaacagtcag gtctttcttc aaagcacttt cagcagaatg
    20641 gaccgcatcc acgacataac cttcactttc aagattaaac tgaaggattt cacagagatc
    20701 ttcttcgtct tctgctacca gtattctgta tttttcaacc atgttaaagg atgatctaat
    20761 tgctaatttt catttctctt ccttattctg atgcctgata tccgtcccat cgagggcata
    20821 gatggtagct tcagccacat tggtcgcatg atcggcaatt ctttcaatgc tggatattat
    20881 actgttagtg tgaatgaaag agagcaatat ctgcctggtc tcttcaggaa ggccggtctt
    20941 ggatatcact ttttttacca gtttttgatt catctcatcc accacaggat cattttttat
    21001 ggtccatatg gcatattcgc ggtcaccctg ggtgaaagac agaatagatt tttgaaccat
    21061 gttctgagta actatcagca tgttataaat aacatctgtc attattccat aaatctcctg
    21121 atcttttatc tttggaataa aattaacgat atttatcacc aaatcgccaa ttctttccag
    21181 attgatgacc atctgataac aagccattaa ttttcgcaaa tccgacgcca aaggttgttg
    21241 aagaacgatg gtattgataa ttttctcgct gagtttaatt tcaaatttat cggatttctc
    21301 ttcattgctc ataatttctt ccaggatatc cgaaggtatt aactgtgatc ctgagttgat
    21361 gattttttca agcagggtga gttgatgaag aatcagttca gaataatcac tgaacttttc
    21421 gatcacctcc agtatggcat tttctttctt tatattcatg gtattctttt attgatgaat
    21481 tcgataattt gaaaattaac caaatttccc ggtaagataa ccttccgttc ggggatcttt
    21541 ggggttggta aacatggttt tggtacggtc atattccacc agttctccca ggtacatgaa
    21601 catggcgtta tcagagatac gtgctgcctg tgccatgtta tgggtaacca gtacgatggt
    21661 atagtttttc ttcagttctg taaacaggtc ttctatcctg gcggtggaca agggatcgag
    21721 tgctgaggtt ggctcatctg caaggatcac ttccggtttc aacgcaagag ccctggcaac
    21781 acacagtctt tgttgctgtc ctccggagag gaaagtccct tttttattca aagaatctct
    21841 gacttcatca aaaagaccca catccataag cgacttttca acgatttcat ccttttcaga
    21901 ccgtttcagg ctgaatccgt tcagttggta tccggcgagc acattagcgt ataccgacat
    21961 ggtggggaag ggatttggac gttggaacac cattccgatc ttccgtcgta atgtggtggt
    22021 tgacatgtta aaaacatcct ccccgttaag ccgtatttca ccttgcatca ctatttcagg
    22081 atataaatca tgcatcctgt taatagccct gagcaaagta cttttcccgc atcccgaagg
    22141 tcccatgatg gccgtgatgg agtgtctttt tatatctgca gtaactcctt ttacggcagg
    22201 tttatctttg gtgtaggcaa ccgaaagatt acggagagag agtgccggat gttcgatgtg
    22261 cattttattt tcagtcataa cccgcaaatc attggttgtt tccaaatcgt gaattaattc
    22321 ttttcgccag aatattcaaa acaagtacaa ctcccatcag aaagagtgag gagctccaga
    22381 cgagcctgac catattggga ttgttgtaaa attcccagat cagtaaaggg acagcgctgg
    22441 tcggtttata gatatttaag tttatcatgg tgctgcccag agctgtgagc ataagagggg
    22501 cggtctcgcc aaggattctg gaaatggaaa gcaggatacc ggtcatgagt ccgctgaaac
    22561 cggtcggtat cataaccttc aggataattc tccagtaggg tactcctaaa gcagctgcgg
    22621 cttctttcag ggttaccggg atcattttca tggtttcctc cgtggaacgg atgacaattg
    22681 gcagcatcat gatagcaagt gccacggaac ctgccagtcc cgaaaaacct ttggtaatgc
    22741 ttatgacgat ccagatgtaa gcaatcagtc ctaatactat agaaggtact ccctgcaaaa
    22801 tgtcggtaag atttctgatc agccgggcat accatccatt gggatgctca tacaggaaga
    22861 tgccagcaag aatgctgaca ggaatggcaa tcagcgaagc tatcaggacc attaaaagcg
    22921 aaccggtaat tccgttggca attcctccgg gtataatttc tccgttgtta acggctgtca
    22981 tggcttccaa aggatcgggt gcgactttga taaaaaagtc gagattaatc tggctgatcc
    23041 cttctttgac cagtttccag agaatcagaa atatgggaag cactgttacg attgacagtg
    23101 tcactaccaa tcctaggaag agcttgtttt ttttatctct ccatgaagag cgatccgggg
    23161 tatttctatt gtcagatttc attaagttca tcaatggata aagcttatct aacatccttg
    23221 tttatgtatt caggcatatc ggcgaacaat ccatttccct gccgcattta ctatggcagt
    23281 aaccacaaac agtaaaaggc caatggcaat aagtgcactt aattttaaac catcagcttc
    23341 tccgaactgg ttggcaatga cagaagccat tgtattcccc ggactgaaaa ttcctttagg
    23401 gatcaggtta gcgtttccta tcagcatggt aactgccatc gtttctccca gtgctcttcc
    23461 tagtgccaga atataaccag ctacaagtcc cgaccttgaa accggcagaa ttacattaaa
    23521 gattacctct aaccgggtag ctcccaagga gtaccctgct tccttcaact cattcggaac
    23581 cattgaaatt atttcgttgc tgagtgaagt ggcgtacgga ataatcatga tcgccagaat
    23641 cagggaagag gtgaatatgc cgtaaccctg ttcagataat cccaggtcca caatgagcgg
    23701 gcgaagggta taaaatcccc ataatccgta aacaatggaa ggaattccgg ccagcagatc
    23761 caccagtgtg ctgagaatac cggcaatttt tgtttttttg aagtattcgg aaatgaacag
    23821 ggaagtggca aaagaaagcg ggaaacaaat gatcaatgcc aaaagtgagg taaccaccgt
    23881 tccgaagata aacgggaagg ccccgtaaac ctctttcccc tccgtaggat tccattccga
    23941 agaaaatata aaccggaaga tgccaaattc cctgaaagcg ggaatggatc cttttaaaag
    24001 cgaaaagata attccggctg caagaagcaa gaccaggaaa gaggcagatt tcaggataac
    24061 cttggctatt ttgtcgctgg tgaatatttc ccgagaccgg atcattgcca cttataacaa
    24121 aggttcgcct tcaaaagtca ctgatttaag aatatcgttg gccagttgga ccgctttttc
    24181 aggcaggggt gcatagtcaa ctttccgtgc aattgcctgg gcatcaggtc caacaagcca
    24241 ctggaggaat ttaacagttt cccgggcctg gtcgattttt cggccattat atgactgttc
    24301 tttatataga ataatccagg taaatgcgct tatgggatag gcatccgggt cagccgaatt
    24361 ggtgaccatt accctggtat cagccggaat atcaccttgg gcagcggcac tgacggattc
    24421 cacagatgga gcaataaaat tacctgcctg attctgaagt aaaacacaag gaattttctg
    24481 agcaaaagca tactccgaac caatatatcc gatggttcct tcagtttgtt tgatgatgcc
    24541 ggctactccc ggatttcctt tggcacccat tcctaccggc cagttcagtg atttccccgt
    24601 tcctacctta tctgcccagg acttactgat tttgctcagg taatcgctga aaatgaaagt
    24661 agttccgctt ccgtcggaac gatagactac cgaaatatct ttgccgggca ggttaattcc
    24721 gggattgtcg gcaaggattt tgggatcatt ccattttttt atttctccca gaaagacaga
    24781 agcaagcacc tcgtttttca tttttagttc agtgattccg ggaagattaa aggcgattac
    24841 cactgctccg agacaggttg gcacatgcac aacaggcgcc ggcagttcgg ctgcctgctc
    24901 atcggtaagg aatgcatcgg ttgccccgaa atcgactact ttgtctttca gactcctgat
    24961 accaccaccc gaaccaatac ctccgtaagt gagtttgttc ccggtcatgg tggagtaatc
    25021 attaaaaact ttgttataga aaggttgagg gaaggtcgca ccggcagctg taatctcagc
    25081 tctcaaactt ccggatttct catttttatt tttattaccg gaacatgcag ttaaagaaat
    25141 acatactatg aatacaaaaa ctaaattctt cataaaatct cacttattaa tttgcaattt
    25201 ctcattttta gaaaactggt aaaaacattt cggcaaattc cgatttttga aaattaactt
    25261 tttaaattgt cctgattgta aagaacttcc gacagtacaa agtaacatca ctcctgttac
    25321 aaattagtta cagaattgtt acaataattt taaatctgcc tgtcaggatt tgatgattac
    25381 ggaattaata ttttgcctgt atgctgataa tcaggatatt ctggttgaca gcttttcccc
    25441 actcgatgcc tttgattatc ctggtgccgt cagcctaagt gtaaaataga gatttaaggg
    25501 cgatcgattt ggtataacca aaataaccgt ggcctccaag gtcaagtttg gctccgattg
    25561 ctctttcccc ggggtaaagg gttgaccacc agggaccatt cgacgacttc ccgttgatcg
    25621 gttgaatatt ccacttcata attgggtcgg ttaaattttc ctatccgtaa actaaaagtt
    25681 ttggtacggt gatcatttac aacagcactg gcatctttaa aagaaaaact tccggaagtt
    25741 aaatcaggtt acaaaaacta tgtgcattgg tactggtagc atttacctgg gcttataagg
    25801 ccggaattca tctctatagt ttgaatccaa taaaaattat ctggcattga agacgagcct
    25861 atagtatttt gtaaatatgg cttgatttat atcgctaata tgttaattat cagtgatata
    25921 gataaatttt agaaagtagt aaattattgt catgtactta gctgagtgaa acaaaacttt
    25981 acaaaaactt aacacctttt ctatcctggt tacaagcaag gtgaata
//
LOCUS       DAOPMW010000014        25329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_1755_length_25329_cov_11.691699, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000014 DAOPMW010000000
VERSION     DAOPMW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 25329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25329
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_25329_cov_11.691699"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..731)
                     /gene="rpsP"
                     /locus_tag="PLU11_01535"
     CDS             complement(282..731)
                     /gene="rpsP"
                     /locus_tag="PLU11_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPN57814.1"
                     /translation="MSAKIRLQRHGSKKRPFYFIVVADSRAPRDGKFIQKLGTYNPLT
                     VPATIQLDRQKALEWLNKGATPTDTVRRILSFKGVLFLKHLLRGVSLGLFDDATAMEK
                     FTKWSAEHEAQVKRRQEEAYRQKRARRVPSTGRRPERRPEGSGGGEG"
     gene            complement(1127..>2317)
                     /gene="ffh"
                     /locus_tag="PLU11_01540"
     CDS             complement(1127..>2317)
                     /gene="ffh"
                     /locus_tag="PLU11_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPN57815.1"
                     /translation="YKIAKEFTDKVKDKAMGEKVINAISPGQLMTKIVKDELAELMGG
                     EEAVFNAKGNPAVILIAGLQGSGKTTFSGKLAHFLKTKKGLSPLLVAADIYRPAAMEQ
                     LRVLGEQIGVDVHIELESKDAVAIATNAVKEARSKNKNVVIIDTAGRLAVDEVMMTEV
                     ANVKNAVNPQEILFVVDSMTGQDAVNTAKAFNERLDFTGVVLTKLDGDTRGGAAISIK
                     YTVNKPIKFTSSGEKMDTLDVFYPERMAQRILGMGDITSLVEKAQAQFDEVEAKKLEA
                     KLRKNKFDFADFKMQLEQIKKMGNMKDLLGMIPGLGSKVKDLDINDDSFKGIEAMINS
                     MTPAERADPDLIDGSRKKRIAKGSGKDIQEVNNFMKQFEQMRDMMKNMNKMGAFGKMM
                     PGRK"
     assembly_gap    2318..2417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2494..3363)
                     /locus_tag="PLU11_01545"
     CDS             complement(2494..3363)
                     /locus_tag="PLU11_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPN57816.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELISPEEEVKLATLIK
                     QGDQRALDRLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVRLPLNKVGLTNRIQKAFSQLEQQYEREPSAEELAE
                     VLNMDIEEVSSTLGINARHVSMDSPLSEGEENTLIDVLENPNAEKTDNELDHNESLKT
                     EIDRSLKTLTDRQKEVICFFFGIGVDHPMSLEDIGERFNLTRERVRQIKDKAITKLKT
                     SNRCKLLRTYLGA"
     gene            3671..4048
                     /locus_tag="PLU11_01550"
     CDS             3671..4048
                     /locus_tag="PLU11_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HPN57817.1"
                     /translation="MSKQLYTLEFPVRCSPSILFEFLSTPAGLGEWFADKVDERDNIF
                     YFGWNGAFEKAEVMEIEQDKYIRYHWLTAPKEEYFEFRIEKSEITNQTILIIKDFAEK
                     KEIKDQSMLWDYQVKELFHRLGN"
     gene            complement(4045..4662)
                     /locus_tag="PLU11_01555"
     CDS             complement(4045..4662)
                     /locus_tag="PLU11_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57818.1"
                     /translation="MKSAKIRLSEEEQLLVYNGDWILTKNRVLDAVSSFLADLQAEQS
                     RWLRDQAPGLPPSILTIPAKVSKGEQYLGLPYRILDYPRIFSSADIFAVRTFFWWGHY
                     FNVTLQLAGHWKKQTEASLIAALADIKEENLYICTGTDPWQHHFTDDYYQPVRTLNSA
                     EWEKIILEQAFIKLSCRQELSDWEQMGERLLADFGRLIRLTGYTG"
     gene            4677..6116
                     /locus_tag="PLU11_01560"
     CDS             4677..6116
                     /locus_tag="PLU11_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPN57819.1"
                     /translation="MFRKLDKLILKAFIGPFIATFFITLLVLVIQFFWLYIDDFVGKG
                     LGPSVIFEFILYQSAVLVPLALPLAILLSSLMTFGNLGESFELVAIKSAGISLLRFMR
                     PLFIVSIFISGIAFVFSNNVIPVANLKSRTLLADIIYAKPAFDLKEGIFYDKISGFAI
                     KIGKKEQNDSVIRDVIIYEQGNPLQDNFIVAKSGIMRVTANKRFLEFNLKDGWRYQER
                     GNVYANSNIEYIRLGFKEYKKQFDMSTLGFSNRTADSVNKNNEKMFTMRQLNVAIDSL
                     EKDQEQTRDRSIRDMFNVLQFNSLLDSAWNKLPPDSTRYSKIKSVDELLPDSARSNIN
                     EMAKSTAGSVKINAESLFQISKDKGRNLRKHQVEWHRKITLSLACIVLFLIGAPLGSI
                     IRKGGLGTPLIFAIVFFMIFYFSSNTGEKFAKEGSMDPFTGMWLATFILLPLGIFLTY
                     KAMRDSQVLNKEFYSRVARFVRKLFRRPS"
     gene            6155..7144
                     /locus_tag="PLU11_01565"
     CDS             6155..7144
                     /locus_tag="PLU11_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPN57820.1"
                     /translation="MVTSKQQQNLRVQKWVAALSVLILAIKFVAYYTTHSVAILTDAL
                     ESIVNVTAGFIGLYSLYVAAKPRDTDHPYGHGKAEFLSAAIEGTLISVAGAIILYKAG
                     ENLIHPVQLHRLDTGIWLVAATALANFLIGYYCLRIGKKNNSIALVASGRHLQSDTYS
                     TIGIILGLVLLYFTGLFWIDSMVAILFGILIMITGYRILRKSIAGIMDEADSDLLGKM
                     VQVLNTNRQVNWIDMHNLRVIKYGAVLHLDCHLTVPWYLNVHEAHREIDSLAALVRRE
                     FGESLELFVHCDGCLPFSCQICKKADCAERKNNFEKEIAWTMDNISTNKKHGL"
     gene            7144..7536
                     /locus_tag="PLU11_01570"
     CDS             7144..7536
                     /locus_tag="PLU11_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPN57821.1"
                     /translation="MKTTTTWKGAHTFQCEHEGNLIAVDGDKVSGHGPKALLLSGLAG
                     CSGIDVVDILEKMRVGFSDFSMETAAEVTDEHPKVFTGVEITYRIKTDPENEDKVKKA
                     IELSLEKYCGVAAMLKKNSAVNYKLVIL"
     gene            7546..7950
                     /locus_tag="PLU11_01575"
     CDS             7546..7950
                     /locus_tag="PLU11_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN57822.1"
                     /translation="MLSKKSQYAFKALEYLTLHYKQGPVLISEISKKKKIPLKFLENI
                     LLELKKAGILDSKKGKGGGYFLSKDPAKVKVATIVRLVNGPIAMLPCVSLYFYERCKN
                     CDEKKCGLHDMMIEVRDASLGILENRTLKDLV"
     gene            8051..8686
                     /locus_tag="PLU11_01580"
     CDS             8051..8686
                     /locus_tag="PLU11_01580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPN57823.1"
                     /translation="MGLHLGDIAPNFRAQTTAGEIDFHEYLGDSWGILFSHPADYTPV
                     CTTELGKTALLQEEFRKRNVKVLAVSVDSLDHHQDWVKDINETQHCNVDFPIIADPDR
                     VVAFLYDMIHPKASETFTVRSLFVIGPDKKIKLTITYPASTGRNFYEVLRVVDSLQLA
                     ATYTVATPADWKEGEDVIVGLSVTTEDAQKTFPKGVKVVKPYLRYTPQPNI"
     gene            8686..9387
                     /locus_tag="PLU11_01585"
     CDS             8686..9387
                     /locus_tag="PLU11_01585"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPN57824.1"
                     /translation="MLTIEQIKEAESLSAAEAIRLVTEWFPQGVVFSSSLGQEDQVLT
                     DMIYKNGLPVKIFTIDTGRLFNETYELLDRTNARYKNNMQVYFPESADVESFVQEKGI
                     NAFYESVENRKECCFLRKVKPLNRALAGAKVWITGLRAEQSDNRQDMPLIEWDEQRQL
                     YKFNPLINWTYEQMLTYIKEHNVPYNRLHDKGFISIGCAPCTRAIETGEHPRAGRWWW
                     ESSQKECGLHGEHKK"
     gene            9384..10319
                     /gene="cysD"
                     /locus_tag="PLU11_01590"
     CDS             9384..10319
                     /gene="cysD"
                     /locus_tag="PLU11_01590"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPN57825.1"
                     /translation="MTSTEQSCMNTTYHLNYLEQLEAESIHIFREVAAQFEKPALLFS
                     GGKDSICLVHLALKAFAPGKIPFPLVHIDTGHNFPEALDFRDAMAASVGATLVVRKVE
                     DTIKQRSLTEPKGKFPSRNWLQTYTLLDTIEEFGFDCCIGGARRDEEKARAKERIFSV
                     RDEFGQWNPKLQRPELWNTYNGKIHKGENVRVFPISNWTELDIWNYIRHEKIDLPSIY
                     FAHERKVIEHEGQLVAISEFVHPDEQDVVMTKRVRYRTVGDMTCTAAVESTADTLDEV
                     INEIISTRISERGETRIDDKVTEAAMEDRKKNGYF"
     gene            10508..11752
                     /locus_tag="PLU11_01595"
     CDS             10508..11752
                     /locus_tag="PLU11_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPN57826.1"
                     /translation="MDLLRFITAGSVDDGKSTLIGRLLYDSKNILIDQLEALEKSTKN
                     RTDGSVDLALLTDGLRAEREQGITIDVAYRYFTTPKRKFIIADAPGHVQYTRNMITGA
                     SNASLIIILVDARQGVVEQTRRHSIIASLLRIKHVVVAVNKMDLVDFSQDVYNNIVID
                     YNEVAAQLGLKNITYIPMSALDGDNIVDASTRMPWYTGKPLLEFLEDVVLDEDVNLTD
                     ARFQVQFVIRPQTEELHDYRGYAGRIISGIYKKGEKVTVLPQGIETTISSVEIAGKEV
                     EEAFAPQSAVLQLADDIDISRGDTIVKSTNLPKVSNELEVLLCWLDDKPLMTGNKYLL
                     QHNSRQVRAMIRQVEYKIDVNTLQQQPVEGQVKLNEVVKATIKTAAPMVYDPYDQLSE
                     YGSAILIDETSNSTVAAVLIKS"
     gene            11752..12576
                     /gene="cobA"
                     /locus_tag="PLU11_01600"
     CDS             11752..12576
                     /gene="cobA"
                     /locus_tag="PLU11_01600"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPN57827.1"
                     /translation="MQTPAHPTSGSITQGLSLVTLAGAGPGDPELITLKLQKRLAEAD
                     VIISDRLVNPAIILAHARPDVRIIQAGKQGYNEHSYTQEEVTAFILEEARKGSKVLRL
                     KGGDVAFFSNVLDELEALVKEKIPFEIIPGITAASGASAYAGIPLTARGYAQGVRFIA
                     FNPNQQFDEATFRSLATGTDTIVVYMAVRNLGELTQQLLSYEADPQRKIAVVEQASTP
                     AQKISISTLKEAVNDFAGKSFTSPSLIIIGDVLSLYGRFDWFKETGGDPVFNELQS"
     gene            complement(12585..14699)
                     /locus_tag="PLU11_01605"
     CDS             complement(12585..14699)
                     /locus_tag="PLU11_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HPN57828.1"
                     /translation="MQSFRTELENPLVEKDILDLEKKIILFREGKMDEEKFRSLRLAR
                     GVYGQRQPGVQMVRIKLPYGKMTLAQWKRIAAVSDEYSTGNLHLTTRQDIQIHFVSLD
                     RTPQLWAELDKDDITIREACGNTVRNITASDTAGIDPEEPFDVTPYAHAMFEYFLRKP
                     LCQEMGRKFKIAFSNTEKDTALTFMHDLGAIARVRTVDGKEQRGFKILIGGGLGAQPH
                     LAICTHEFLEEDLLIPYLEAVLRVFDRYGERNSRHKARIKFLIQKIGIETFNKLVEEE
                     RLALTHQRVAIRSRETGVGSRETGVGRRESGDDHIDPLKYELWKNTNLFKQKQEGYYA
                     VYVRVPNGNISSDISRRLIGQLEGVIADDVRVTINQGLQFRFVKPEHLPYVYQVLESE
                     GLAAPGFGSVADITSCPGTDTCNLGISNSTGTALALSEVIEEAFPEFLFNKDITVRIS
                     GCMNSCGQHGMASVGFHGSSLKAKGQVVPALQVLIGGAVLGSGDARISDKVIKVPSKR
                     APAVLISIFNDYKKLAAENESFTDYSLRKGEKYYYELLKPLASLDTLSDDEFIDWGQE
                     AKFATAIGVGECAGVMIDLVSTLILEAEEKIVGAEDCLRNNQWADSIYNAYAAQVHSA
                     KALLLQQGVHCNTQIGILNDFDTHFTAKGLYKENGSFKQEVMRMNETTPSAAFAAEFI
                     QQALDFIQFAKSWREKTVKEEK"
     gene            14928..16190
                     /gene="hemA"
                     /locus_tag="PLU11_01610"
     CDS             14928..16190
                     /gene="hemA"
                     /locus_tag="PLU11_01610"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HPN57829.1"
                     /translation="MEYQQLFTDQLNRLKDEGTYRYFLEVNKSAQHFPRFTYQDEQGN
                     SQYAINWCSNDYLGLSSEESLISKLGFAAHRSGTGSGGTRNISGTTNYHKELERTLAD
                     WHRKEGALLFNGAYQANVTTLQTLGRQLPGLVFFSDERNHASIIEGIRGCRNEKKIFA
                     HNDMEQLEELLKQVPAEQPKLIVFESVYSMSGTIAPVNAIIALARKYNALTYVDEVHA
                     VGLYGKTGAGILEEENVQSSIDIVNGTLSKAIGVFGGYIAASSVLIDFIRSFGRGFIF
                     TTSLPPAICAAANTSIQLIQREEQRRRQFFSNVAYLRQSLYENGVDFKLNPSHITIIP
                     VAGADRCRELARQLLEKQGIYLQPINSPTVPVGEECLRVICTARHLPKHIHHLALSLK
                     KLLHGDYQTDRQEFPSFALAAGDCEGQD"
     gene            16114..17640
                     /gene="hemC"
                     /locus_tag="PLU11_01615"
     CDS             16114..17640
                     /gene="hemC"
                     /locus_tag="PLU11_01615"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPN57830.1"
                     /translation="METIRLIGRNSRLSLLQLEIVKARIEQVFPEVKVELIARSSRGD
                     QLSGIPLHTVEGQDFFTRDIFEALSTHEADIAVHSLKDMSSAHFFGDHLFAVVDRDDT
                     RDLAIFNPGILDKISRGETIIIGTCSPRREEMATVFLKKALPQLKETIHIETRPIRGN
                     VESRLRKLDSGDYDATILATAGLNRLLRSPADSAMIQQLLANKKLMLLPLIECVPAPC
                     QGAIVAEAHPSNHRAADILKAINNPMLLQDAIAEKRTAVQYGTGCLQQFGVTTIKTKQ
                     GACLFAAGKDDQSIAFTDWQPWPALPIAPAHIFSTTDRMRDFFNYEWRAALPEINLPV
                     VFIANYKLMAGVINKNALQNKTILVSGTKTWYELARQGYWVTAGADALGFEYLLPSLS
                     MPLLKIEANDILILTHEAAAQRWQKKGYSAACTYKLIPVFNPSLFEEIAGAKAICWSS
                     FAQYDAYGHLAQPGITHICAGGETATQLKQAGIDPVIFPTIKAFEQWRKLNTLSPNAA
                     "
     gene            17601..18596
                     /gene="hemB"
                     /locus_tag="PLU11_01620"
     CDS             17601..18596
                     /gene="hemB"
                     /locus_tag="PLU11_01620"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPN57831.1"
                     /translation="MEKIEYPLTQRRLRTSSLVRELTASVKLNHKSFILPLFVEEGLA
                     APRPVPGLGPVLVETIHSITGVVEKQLANGIAKFLLFPVPAGKQEQLKDFSFATNLVR
                     TLKQTFSERIWIAADLCLCSYTTHGHCGVLDEEHTRLLNSPTVDVLVQYASKLAAAGV
                     DCIAPSDMTDGRIGAIRTRLDALGFEEVTIMSYAAKFSSQFYGPFRDACHSAPQSAGL
                     QNRKTYQHSPLNPADALASASRDEREGADILMVKPAALYTDIIASVKASTEKPVAAYH
                     VSGEYAAIEALAEKGLVNRAAAHLEVWTALSRAGADIIISYAAGEARQWIKEMEY"
     gene            18723..20228
                     /locus_tag="PLU11_01625"
     CDS             18723..20228
                     /locus_tag="PLU11_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPN57832.1"
                     /translation="MRKNKKEIKEQVPGTKNPLYPVFLKLDTLELLLVGAGNVGLEKL
                     QSLLGNAPQARITVVAPKILDEVRVLLAEHPSCQLKEREFVEADLEHKDLLVLATDNP
                     ELHREVKQIANAKGILVNVADTPELCDFYLGSIVQKGNLKIAISTNGKSPTAGKRIKE
                     VLQDALPGELDQVIENLHILRGRLNGNFQHKVKKLNKITRVMVENESSSTAQRWRKIA
                     TYSLIGFALMLIGHFIFSYIPVRQIADDTSAWYKTLDPNFHWMVLAGFMAQMVDGATS
                     MGYGVTSSIVLQSAHVSPAAISGGIHTAEMFTTAASGYSHYKFGNVNKKLFKALVIPG
                     IIGAILGAFLLVKFGETHIRFLRPAMAIYTLLLGVRIFSNAFRAVDIKKKFKRFGWLA
                     GAGGFLDSFGGGGWGPIVTSTLITKGRTPRYVVGSVSLTEFFVTLASALTFFTMIGVQ
                     NWQVILALVLGGVVAAPIAARLAGKLPKKTSFIFLGTVVVLWSFRILYKAF"
     gene            20370..21125
                     /locus_tag="PLU11_01630"
     CDS             20370..21125
                     /locus_tag="PLU11_01630"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPN57833.1"
                     /translation="MNIVRLSFFSLLFYGSCFNGEAQTKQTEQAEQVWLGYLNQTRLS
                     NKWGIWVDAHLRTKDDFVSELNTGIFRTGLTYYMNDDLKLTAGYAYVNHFPGDNHKNI
                     SQPEHRPWQQVQWHTKYPRLRLMQYLRLEERWRRKVLNDNELADGYTFNFKFRYNFLA
                     QFPLGKIRFQPRSLSLVLNDEVHVNFGKQIVYNYFDQNRFFTGLNYQLSGKENLQVGY
                     LYVFQQLGAGNRYKNIHGARIFYFQNLDLRKKK"
     gene            complement(21132..21887)
                     /locus_tag="PLU11_01635"
     CDS             complement(21132..21887)
                     /locus_tag="PLU11_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN57834.1"
                     /translation="MKLFSAKHTAPRFTDEGQGDPIILLYGLFGSVKNFQPLIEHLRK
                     THRVIVPIFPFYELGFCVDIFTLMKFVQEVADELQLEKFHLLGNSMGGHIALLYTLQH
                     PEKVKTLTLSGSSGLYEHGMGDTYPRRRDYDYIKTKTELTFYKPEVATKELVDEIYAT
                     VNSRKAIQIISLAKSTIRHNVEKDLHKLTLPCCLIWGKNDTITPPEVAESFHRLMPQS
                     ELHWIDECGHVPMLETPEAFNPLMDDFLKRSSA"
     gene            22185..23363
                     /locus_tag="PLU11_01640"
     CDS             22185..23363
                     /locus_tag="PLU11_01640"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HPN57835.1"
                     /translation="MAKQQKPATRAVRMQTPKTAQKEHSTPLFLTSSFTYDSAEDMAA
                     AFATESPEVNIYSRFSNPGVDEFVNKMAALEGAEHGVATGTGMAAIFSTFMTFLSKGD
                     HIISASAVFGSTHTILTKYLPKWGIESSYFDMNQPETIETLVKPATKMLYLETPSNPG
                     LDIIDIEYVAAICKKHSIMLVVDNCFATPAVQQPIQYGADLVIHSATKFIDGQGRVLG
                     GVIVGKKELVHQLYLFIRNTGPSMSPFNAWVLSKSLETLFIRMDRHAASALAIATKLQ
                     GHAKISRVKYPFLESHPQYAIAKKQMSNGGGIVTFELKGGVESGRKFLNALEMISMTN
                     NLGDSRTIASHPASTTHSKLSEAERQSVGITPGLIRVSVGLEDVEDILDDLLQALDNS
                     "
     gene            complement(23364..24086)
                     /locus_tag="PLU11_01645"
     CDS             complement(23364..24086)
                     /locus_tag="PLU11_01645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPN57836.1"
                     /translation="MNLKDSIIAETENWVAINKPSGLLSIPDREGKEVSLKVLLKEKY
                     GEIFTVHRIDRDTSGLIVFAKNESAHKHLCKQFEDRHTKKIYQGLVIGSPAQPAGSIE
                     APLMEHPGLNGTMIVHRKGKEALTDYEVLTDFGIFSHVQFRIHTGRTHQIRVHMKDLG
                     YPIVCDPLYGDGKPLLLSAVKSKFKLSKNELEERPILGRLALHAFQLSFTDIDGNIVD
                     LEAPLHKDMRATLQQLAKRKKK"
     gene            complement(24110..25060)
                     /locus_tag="PLU11_01650"
     CDS             complement(24110..25060)
                     /locus_tag="PLU11_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPN57837.1"
                     /translation="MKQPGNNFGKFAKKKSNAAIKEQFKQEKRKDKKEREAFFDQKKA
                     EARQRLTPNVNQQSGKKGQAPAPVSHQRKESDELMPLNKYIAHSGVAARREAAELVKQ
                     GLVKVNGETVNEPGFKVSPKDEVRVNGKKIFLAKNLVYILLNKPKDFITTTDDPQGRK
                     TVLDIIARATTERVYPVGRLDRNTSGVLILTNDGELAQKLTHPSNEVKKVYHVTLNKP
                     LEKRDFEQILNGVTLDDGPANVDVLAYADSKDKSQVGVEIHSGRNRIVRRIFEKMGYD
                     VRNLDRVVFAGLTKKNVDRGKWRFLTEKEVRDLKHFGKGK"
BASE COUNT         6572 a         5782 c         6068 g         6807 t
ORIGIN      
        1 gaatgtctta taaagctttg cgggaaattt tacgcaagac taagagtaag aattgactaa
       61 attcttcgag tcttctaagt cccccttcgg gggacctgcc taccggacag gcaggtttag
      121 ggggccttcg tgttcatccg tgtgatccgt ggcacccggc tgtctcagac agctatttgc
      181 acagcaaatc cattcgtgcg ttttcgtgtg ctttagtggc caaacaaaaa aatcccggcc
      241 aacggccggg attcatgaac tttcaggatg cggacatttt attatccttc accaccgcca
      301 ctgccttctg gtctgcgctc aggtctgcga cctgtgctgg gaactcgtct ggctcttttc
      361 tggcggtagg cctcttcctg gcgtcttttc acttgtgctt cgtgctctgc actccacttc
      421 gtgaatttct ccatggctgt agcatcatca aacagtccga ggctaacgcc acggagcagg
      481 tgtttcagga acaacacgcc cttgaagctg aggatacggc gaacagtatc ggtgggtgta
      541 gcacctttgt tcagccactc caatgccttt tgacgatcca gctgaatagt agcaggaaca
      601 gtcaggggat tgtaagtacc taatttctgg atgaatttac cgtcgcgggg tgcccgtgaa
      661 tcggctacaa caatgaagta aaagggtctt ttcttagacc cgtgtctctg aagacggatt
      721 tttgctgaca taaatagaca tttaacagcg ttaaacaata attgttagtt ccggtagata
      781 ccggagagcg aagataggaa tttgatcgga aaatgcaaca aggttttggt tcagtctgca
      841 gtaggcaatc ggcagcaggc agcccttcct tacaggaata gttaaaacct taaatgcttt
      901 gatttttgaa cctgtttgtc agcacaaggc gttgattaat agtgttttat gaatattata
      961 aaggtagaga aatgaaagat ttttctgcct ttattaatta gtcgcctgaa gagtattcaa
     1021 ttaatgaatg agcctttgaa atatgcaaaa tccaggttta ttcctttgaa gcaaaaattt
     1081 gtgactgccg actgccaact gccaactgcc gactgccaac tgccgactac ttcctccctg
     1141 gcatcatctt cccaaacgcc cccattttat tcatgttctt catcatgtcg cgcatctgtt
     1201 caaactgttt catgaaattg ttgacctcct ggatatcctt accgcttcct ttggcgatcc
     1261 gttttttgcg gctgccgtcg atcaggtccg ggtcggcacg ttcagccggc gtcatcgagt
     1321 tgatcatggc ttcaatgcct ttaaatgagt catcattaat gtccagatct tttaccttgc
     1381 tgcccagtcc gggtatcatc cccagcaggt ccttcatatt ccccatcttc ttgatctgtt
     1441 ccagctgcat tttgaaatcg gcaaaatcga atttattctt ccggagtttg gcttcaagtt
     1501 ttttggcttc tacttcgtca aactgcgcct gtgctttttc cacgagtgaa gtgatatcac
     1561 ccatgccgag aatacgctgg gccatccttt cgggataaaa cacatccagg gtatccattt
     1621 tttccccgct gctggtaaac ttgatcggct tattaaccgt gtacttgatc gaaatagccg
     1681 caccaccccg ggtatcgcca tccagctttg ttaataccac accggtaaag tctaaccgct
     1741 cattaaaggc cttggcggta ttcaccgcat cctgccccgt catactgtcc accacaaaga
     1801 gaatctcttg tggatttacg gcattcttta cattggcgac ttccgtcatc atcacttcat
     1861 ccacggccag gcggccggcg gtatcgatga tgaccacatt tttattttta ctgcgggctt
     1921 cttttaccgc attagtggca atggccaccg catccttact ctccagttca atatgtacat
     1981 ccaccccgat ctgctcaccc aatacccgta attgttccat ggccgccgga cgataaatat
     2041 ctgccgccac cagcagggga gagaggcctt ttttggtttt gaggaaatgg gccagtttac
     2101 cactgaaagt ggttttacca gaaccctgca ggcctgcgat caggatcacc gccggattgc
     2161 ccttggcatt gaaaacggct tcttcgccgc ccatcagttc agccagttca tcctttacaa
     2221 ttttggtcat caactgaccg ggagagatcg cattgattac cttttcaccc atcgctttat
     2281 ccttcacttt atccgtgaat tccttcgcga ttttatannn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnngcc gaagacaaaa gaaaaaagtg aaccgaagtt cactttttaa
     2461 cgagttgcaa agataggtca agtagagttt atatcatgcg cctaaatagg ttcttaatag
     2521 tttacaacga ttggaggttt tgagcttggt gattgcttta tccttgatct gacgaaccct
     2581 ttcgcgggtc aggttaaaac gttccccgat atcttccagg ctcatcggat ggtccacccc
     2641 gataccaaaa aagaagcaga taacctcttt ttgacggtcg gtgagggtct tgagcgagcg
     2701 gtcaatttcg gttttcaggc tctcattatg atccagttcg ttatctgttt tttccgcatt
     2761 cgggttctcc agcacatcaa tcagggtgtt ttcctcgcct tcagacaacg gggaatccat
     2821 actcacgtgc cgggcattga taccgagggt ggaagatact tcctcaatat ccatattgag
     2881 tacttcggcc agttcttcgg cagaaggttc ccgctcgtat tgttgttcca gttgggaaaa
     2941 ggctttctga atccggttgg tcagtcccac tttgttcagg ggcaaccgca caatccgggc
     3001 ctgttcggcc agtgcctgta aaatgctctg gcggatccac cagaccgcat aggaaataaa
     3061 tttaaaacca cgggtttcat caaaacgctg ggcggctttg attagcccca ggttaccctc
     3121 attgatgagg tcggggagcg aaagaccctg gttctggtac tgtttggcca cagatactac
     3181 aaaacgcaga ttggcctttg tcagccggtc caatgcacgc tggtcgccct gtttgatcag
     3241 ggtcgctaat ttgacttctt cttccgggga gataagttct accttgccga tttcctgcag
     3301 gtatttttcc aggctttggg actcacgatt ggtaatagac ttcgtgatct tgagttgtct
     3361 catgctcata ggtatggttt taaatggtgt attttaaggg ttaaagaata agtttggttt
     3421 ggtcttatac tctaaaaata cccaccacag tgtctaggtt gtacagaaag tattgaactt
     3481 caagggcaat ttaacgtaat tgcccatacg cgccaaatta attttggcat cctattaacg
     3541 aattcatcct caatttattt tgtcaaaaca gttgcggaaa caaatagggg gaaaaataag
     3601 tgctaaaata ttttgcagca atgatttatt ttctattatt gcagctatcg cgatgaagtg
     3661 atagaaacca atgagtaaac aactatatac cttagaattc ccggtccgct gctccccgtc
     3721 catcttattt gaattcctct ccaccccagc cggtcttggc gagtggtttg ctgataaagt
     3781 agatgaaaga gacaatatct tttattttgg ctggaatggg gcttttgaaa aagccgaagt
     3841 gatggaaata gaacaagata aatatatacg ttatcactgg ctgacggccc ccaaggagga
     3901 atattttgaa ttccggatcg aaaagtctga gattaccaat cagacgatcc tgataattaa
     3961 ggattttgcg gaaaagaaag aaatcaagga tcagagtatg ctttgggact accaggtgaa
     4021 ggaactcttt caccgcctcg gcaactaacc cgtatacccg gtcaaccgga tcaaacggcc
     4081 aaaatcagcc agcaatcgtt cccccatttg ttcccagtcc gacagttcct gccggcagga
     4141 cagctttata aaagcctgtt ccaggattat tttttcccat tcagcactgt ttaaggtccg
     4201 gaccggctga taatagtcat ccgtaaaatg atgctgccag ggatctgtac cggtacagat
     4261 atacagattt tcctctttta tatcagccaa tgcggctatc agggaagcct cggtttgctt
     4321 tttccaatgt ccggccagtt gcagggtaac attgaaataa tgcccccacc agaaaaaagt
     4381 gcggaccgca aatatatcgg ctgaactaaa tatgcgcgga taatccagta tccggtacgg
     4441 taagcccagg tattgctcac cttttgaaac tttggcggga atagtaagta tcgaaggggg
     4501 aagacctggt gcctgatccc ggagccaccg ggactgttcc gcctgaaggt ctgccagaaa
     4561 agaactaacc gcatccagta ccctgttctt tgttaaaatc cagtctccgt tgtacaccag
     4621 taactgctcc tcttccgaaa gccttatttt tgctgacttc atttattaaa gataacgtgt
     4681 tcaggaaatt agataaactc atccttaagg cctttatcgg tccttttatc gcaacgttct
     4741 ttattacgtt gctggtattg gtgatccagt ttttctggct ttatatcgac gattttgtcg
     4801 ggaaaggact ggggccatcc gttatttttg agtttatcct ttatcagagt gccgttctgg
     4861 tacccctcgc actgcccctg gcgatcctcc tttcttccct gatgactttt ggtaacctgg
     4921 gtgagagttt tgaactggtg gcgattaaat cggctggtat ttccttgtta cgttttatgc
     4981 ggccgctgtt tatcgtcagc atttttatca gtggcattgc ctttgttttt tccaataatg
     5041 tgattccggt agccaacctc aaatccagga ccctgctggc tgatatcata tatgccaaac
     5101 cggcttttga tcttaaggaa ggtattttct atgataagat cagcggcttt gctatcaaga
     5161 tcgggaaaaa ggaacagaat gacagtgtga tccgggatgt gatcatctac gaacagggaa
     5221 acccgctaca ggataatttt atcgtggcaa aaagcgggat catgcgggta acggccaata
     5281 aacgattcct tgaatttaac ctgaaagatg gctggcgtta ccaggaaaga gggaatgtat
     5341 atgccaattc caatattgaa tatatccggc tcgggtttaa ggagtataaa aagcagtttg
     5401 acatgagcac cctggggttc tccaaccgaa cagcagacag tgtaaataag aacaatgaga
     5461 aaatgtttac catgcgccag ctcaatgtgg ccattgattc gctggaaaaa gaccaggaac
     5521 agaccaggga ccggtccatc cgcgatatgt ttaatgtact gcagtttaac agcctgctgg
     5581 attcggcctg gaataaactg cctccggaca gtaccaggta cagtaagata aaatcagtcg
     5641 atgaactttt gcccgattcc gcccgttcaa atatcaatga aatggcaaaa agcacggcgg
     5701 gttcggttaa aattaatgcg gaatcgctgt tccagatttc aaaggataaa ggccgcaacc
     5761 tccgtaagca ccaggtggaa tggcaccgga aaatcaccct ttccctcgcc tgtattgtac
     5821 tctttctgat tggtgcgccc ctgggttcaa tcatccggaa aggaggatta gggacaccgc
     5881 tgatttttgc gatcgtcttt ttcatgatct tctatttctc gagtaatacc ggcgaaaaat
     5941 tcgccaaaga aggcagtatg gatccgttta ccggtatgtg gctggcgacc tttatcctgc
     6001 tgcccctggg tattttcctg acctataaag ccatgcgtga ttcgcaggta ttaaataaag
     6061 aattttattc ccgtgttgcc cgctttgtac gtaaattatt cagacgccca tcctgacata
     6121 gggatttgta actttgccaa aaccggtact atcgatggtc accagcaagc agcaacagaa
     6181 tctacgggtc cagaaatggg tagctgcttt gtcggtactg attcttgcca tcaaatttgt
     6241 ggcgtattac actacccatt cagtagccat cctgaccgat gcattggaaa gtatcgtcaa
     6301 tgttacagcc ggatttatcg gtttgtatag tttgtatgtg gccgctaaac cccgggatac
     6361 cgatcatccc tacggacatg ggaaggccga atttttatct gcagccattg agggtaccct
     6421 gatctccgtt gccggtgcaa tcattttata taaagcgggt gagaacctga ttcatccggt
     6481 acagttgcat cggctggata ccggaatctg gctggtggcg gccacggcac tggctaattt
     6541 tttgattggc tattattgcc tccggatcgg taagaaaaac aattcaatcg cactcgtagc
     6601 cagcggaaga cacctgcaaa gcgatacgta ttcaacgatc gggattattt tgggactggt
     6661 tttactctat tttacgggtc tgttctggat tgacagtatg gtagccatct tgttcggtat
     6721 actgattatg attacaggat accggattct ccgcaaatcc attgccggca ttatggatga
     6781 agcagattcg gacctgttgg gaaaaatggt gcaggtactg aatacaaaca ggcaggttaa
     6841 ctggatcgat atgcataacc tgcgggtgat taaatatggt gcggtactgc acctggactg
     6901 tcacctgacc gttccctggt acctgaatgt acatgaagcc caccgggaaa ttgatagcct
     6961 tgccgcactg gtacgccggg aatttggcga atcgctcgaa ctctttgtgc attgcgatgg
     7021 ctgcctgccg ttttcctgcc agatctgtaa aaaagcggat tgcgcggaaa gaaagaataa
     7081 ttttgagaaa gaaattgcct ggacaatgga taatatttca accaataaaa aacacggatt
     7141 ataatgaaaa ccaccaccac ctggaagggg gcacatacat ttcaatgcga acatgaaggc
     7201 aatctgattg ccgtagatgg agacaaagta agcggacatg gccccaaagc cctgctttta
     7261 tccggactgg ccggttgttc cggtattgat gtggtcgata tcctggaaaa aatgcgggtc
     7321 ggtttttctg atttttcaat ggaaacggct gctgaagtaa ccgatgaaca tcccaaagta
     7381 tttacaggcg tggaaattac ctaccggatt aaaacggatc ctgaaaatga agacaaggtt
     7441 aaaaaagcga ttgaactgtc attggagaaa tattgcggag tggcggcgat gctgaaaaaa
     7501 aattcagcag tcaattataa attggtcatc ctgtaatcaa tcgctatgct gtcaaaaaaa
     7561 tcgcaatacg cattcaaggc cctggaatac ctcaccctgc actacaagca gggacccgtt
     7621 ctgatctcag agatttcgaa gaaaaagaag atacccctga aattcctgga gaatattctg
     7681 ctggaactga aaaaggccgg catcctggac agtaagaaag ggaaaggcgg ggggtatttc
     7741 ctgagcaagg atccggctaa ggtaaaagta gccacaattg tgcgtcttgt gaatgggcca
     7801 atcgccatgc ttccttgtgt aagtctctat ttttatgaac gttgtaagaa ctgcgatgaa
     7861 aagaaatgcg gattgcatga tatgatgatt gaggtgcggg atgcatcgct cggaatattg
     7921 gagaacagaa cattaaagga cctggtatag ccatccggga cgaacctttg tttgtgccgt
     7981 tcttttaagt ctatcaactt ggtaggttat tgtttttata gtacttttgt catcttaaac
     8041 ttttagcatt atgggactac atctgggaga cattgcaccg aatttcaggg cacagacaac
     8101 cgcgggggaa attgattttc atgagtacct gggggacagc tggggtattc tgttttcaca
     8161 cccggcggat tatacaccgg tatgtacgac agaacttggt aaaacagctt tattacagga
     8221 agagttccgg aagcggaatg tgaaagtact ggcggtgagt gtggattcgt tggatcatca
     8281 ccaggactgg gtgaaggata tcaatgaaac acagcattgt aatgtggatt tccccatcat
     8341 tgcggacccg gacagagtcg tggcttttct gtatgatatg attcatccca aagcatctga
     8401 aacattcacc gttcgttccc tttttgtaat aggaccagat aaaaagataa aactgacgat
     8461 tacctatccg gcttccaccg gacgtaattt ctatgaagtg ttacgggtgg tggattcatt
     8521 acaactggca gccacctata cggtagctac tccggcggat tggaaagaag gagaggatgt
     8581 gattgttgga ttatccgtaa ctacggaaga tgcacaaaag acttttccga agggtgtgaa
     8641 agtagtcaag ccgtatctgc gttatacccc gcaacccaat atctgatgct gacgattgaa
     8701 caaataaaag aagcggaaag tctgtcggca gcagaagcga tcaggcttgt gactgaatgg
     8761 tttccccagg gtgttgtttt ttcgtcctcg ctaggacagg aagaccaggt actcactgac
     8821 atgatttata aaaacggact gccggtaaag atttttacaa tcgataccgg ccgcttgttt
     8881 aatgagacct atgagttgct ggatcgtacc aatgcccgtt acaaaaataa tatgcaggtc
     8941 tatttcccgg aatcagctga tgtggaatca tttgttcagg aaaaagggat caacgcgttt
     9001 tatgaatcag tggaaaaccg gaaggaatgt tgtttcctgc gtaaagtgaa acccctcaac
     9061 cgggcccttg caggtgctaa agtctggatt accggtttaa gggcggagca atcggacaac
     9121 cggcaggata tgcctttaat tgaatgggat gaacaacggc aattatataa attcaatccg
     9181 ctcatcaact ggacctatga acagatgttg acgtatatca aggagcataa cgtgccatat
     9241 aataggttac atgataaagg ctttatcagt attggctgcg ccccatgcac ccgggcaatt
     9301 gaaaccggcg aacaccccag ggcaggccgc tggtggtggg agagttcgca aaaggaatgt
     9361 ggtctgcatg gagagcataa aaaataacaa gtacagaaca gtcgtgtatg aatactactt
     9421 atcatttgaa ttatctcgag caactggaag cggaaagcat ccatattttc cgggaagttg
     9481 ccgcccagtt tgaaaaaccg gccctgcttt tcagcggggg aaaagattcg atctgcctgg
     9541 ttcacctggc acttaaagcc tttgcacccg gcaagattcc attcccgctc gtacatattg
     9601 ataccgggca taattttccc gaggccctgg attttcgcga tgcgatggct gcttccgtcg
     9661 gggctaccct ggtggtacgg aaagtggaag acacgatcaa acaaagaagc ctgaccgagc
     9721 ccaaggggaa gtttcccagc cgcaactggc tgcagaccta taccttactc gatactattg
     9781 aagagtttgg ttttgattgt tgtattggtg gtgcccgtcg ggatgaagaa aaggcaagag
     9841 ccaaagaacg aatattctca gtacgggatg aatttggtca atggaacccc aaactccagc
     9901 gtccggaact ctggaatacc tacaatggta aaatacataa aggggagaat gtacgggtat
     9961 tcccgatcag taactggact gaactcgata tctggaatta tatccgccac gaaaagattg
    10021 atctgccttc catttatttt gcgcatgaac gcaaagtgat cgaacatgaa ggacagttgg
    10081 tggcaatcag tgaatttgtt catccggatg aacaggatgt ggtgatgacc aagcgggtcc
    10141 gttaccgcac ggtaggggat atgacctgta cggccgcggt ggaatccaca gcggatacac
    10201 tggatgaagt gatcaacgaa attatttcca cccggatcag cgaaagggga gagacaagga
    10261 ttgatgataa agtaacagaa gcggcgatgg aagaccggaa gaagaacggc tacttttaaa
    10321 gtaaaaagta acaattaaaa agtaaaaaag cctaaggctg aacaggctga ctgatttgga
    10381 ttttaatatg ggttgaaatt ttaattcttt aacaaaataa aataagtgaa acttcaggta
    10441 taaaagacgt tgcgatctga atggttgtac gcttttttac ttttgacttt ttacttctga
    10501 ctttcatatg gatttattgc gatttataac tgcaggcagt gtggatgatg ggaagagtac
    10561 cctcatcgga cgattattgt atgacagtaa gaatattctg attgatcaac tggaagcgtt
    10621 ggagaaatcc accaagaacc ggacggatgg atcagtggac ctggcgttgc ttacggacgg
    10681 actgcgtgct gaaagagaac agggaattac gattgatgtg gcgtatcgct acttcacgac
    10741 acccaaacga aaatttatca ttgcggatgc accgggtcac gtacagtata cccgcaatat
    10801 gatcaccggg gcttctaatg ccagcctgat catcatcctg gtggatgccc ggcagggggt
    10861 ggtggaacaa acccgccgtc actccatcat tgcttcactg ttgcggatca aacacgtagt
    10921 ggtggccgtc aacaaaatgg acctggtgga tttttcacaa gatgtatata ataatatcgt
    10981 aatcgattat aacgaagtgg ccgcgcagct gggattgaaa aatatcacct atatccccat
    11041 gagtgcattg gatggggaca atattgtgga tgcttccacc cgcatgccct ggtataccgg
    11101 gaaacccttg ctcgagttcc tggaagatgt ggtgctggat gaggatgtga acttaaccga
    11161 tgcaaggttc caggtacaat ttgtgattcg tccgcagaca gaagaactgc atgactaccg
    11221 cggctatgcc ggccgtatca tcagcggtat ttataagaaa ggggagaagg tgacagtgtt
    11281 gccccagggt attgaaacca ccatcagttc ggttgaaatc gctggtaagg aagtagagga
    11341 agcctttgcc ccgcagagtg ccgtactgca actggccgat gatattgata tcagccgggg
    11401 agatacgatt gttaaatcga cgaacctgcc caaagtgagc aatgaactgg aagtgttgct
    11461 ttgctggctg gatgataaac ccctgatgac gggaaataaa tatttgttgc aacataacag
    11521 tcgccaggtc cgggcgatga tccggcaggt ggaatataaa attgatgtta atacactgca
    11581 acaacaaccc gtggaaggac aagtgaaact caatgaagtg gtgaaagcca ctatcaaaac
    11641 agcagctccg atggtgtatg acccttacga ccaattgagc gaatatggtt ccgctatcct
    11701 gattgatgag accagtaaca gtacggtggc ggccgtactt atcaaatcat aatgcaaacg
    11761 cctgctcatc cgacatccgg ttccattacg cagggactgt cgcttgtcac actggccgga
    11821 gcgggaccag gtgatccgga actcattacc ctgaaattac aaaagcgcct ggcggaagct
    11881 gatgtaatca ttagtgaccg gttggtgaat ccggccatca tacttgcaca tgcccgtccg
    11941 gacgtacgca ttattcaggc aggtaagcag ggatataacg aacattctta tacacaggaa
    12001 gaagtcactg cctttattct ggaagaggcg cgtaaaggca gtaaagtact gcgtcttaaa
    12061 ggaggtgatg tggctttttt cagtaatgtg ctggatgaac tggaagcctt ggtaaaagaa
    12121 aaaattcctt ttgaaattat tccgggtatt accgcggctt ccggtgcgtc cgcttatgcg
    12181 ggcattccat taacggcgag gggctatgca caaggcgtgc gatttattgc gtttaatccc
    12241 aatcagcaat ttgacgaagc tactttccga tccctggcta ccggtacgga tacgatagtg
    12301 gtgtatatgg cggttcgtaa cctgggtgag ctcactcaac agctactgag ctacgaagcc
    12361 gatccgcagc ggaaaatcgc ggtggtggaa caagcttcta caccggcaca aaaaatcagt
    12421 atatccacct tgaaagaagc cgtgaatgat tttgccggca aatcttttac gtcaccgtcg
    12481 cttatcatta tcggcgatgt gctgtcgttg tatggccggt ttgactggtt caaagaaacc
    12541 gggggtgacc cggtattcaa tgaactgcag tcgtaagtgg tcagttattt ttcttccttc
    12601 acggtttttt ctctccagct tttagcaaat tggataaaat caagggcctg ctgtataaat
    12661 tcggcggcaa atgcggcaga tggagtggtt tcattcattc gcatcacttc ctgtttgaac
    12721 gaaccgtttt ctttgtacaa cccttttgcc gtgaaatgtg tgtcgaaatc attgagaatt
    12781 ccaatctgtg tattgcaatg aacgccctgt tgcaataaaa gcgctttggc ggaatgcacc
    12841 tgggccgcat aggcattgta aatactgtct gcccattggt tattacggag gcaatcttca
    12901 gcgcctacta ttttttcttc cgcttccaga atcagggtgg ataccaggtc aatcattaca
    12961 ccggcacatt cacccacgcc gatagcagtg gcgaattttg cttcctgccc ccagtcaata
    13021 aactcatcat cagaaagagt atccaggctg gccagcggtt ttaataattc gtaataatat
    13081 ttctccccct tacgtaaact ataatctgtg aaactttcat tttcagcggc cagtttttta
    13141 taatcattga agatgctgat taaaacagcc ggggctcttt tactgggaac cttaattact
    13201 ttatcagaaa tcctggcgtc cccactgccc aatactgctc cgccgatgag tacctgtaaa
    13261 gccggcacta cctgtccttt ggcttttaaa gaacttccat gaaatcctac agatgccatc
    13321 ccgtgctgac cacaactgtt catacagcca ctgatacgga cggtaatatc tttattgaat
    13381 aaaaattcag gaaaggcttc ttcaatcact tcacttaagg ccaacgctgt accggtactg
    13441 ttactgatcc ccaggttaca ggtatccgta cccggacagc tggtaatatc cgccacactg
    13501 ccgaaaccgg gtgcagcgag tccttccgat tcaagtacct ggtatacata gggcaggtgt
    13561 tcgggtttta caaaacgaaa ctgtaatccc tggttgatcg tcacccgcac atcatccgcg
    13621 atcactcctt ccagctgacc aatcaaccgg cggctgatat ccgaagaaat attcccattc
    13681 ggcacccgga catacacggc ataatatcct tcctgctttt gtttaaacaa attagtattc
    13741 ttccacaact catatttcag tggatcgata tggtcgtctc ccgactcccg tctcccgact
    13801 cccgtctccc gactcccgac tcccgtctcc cgactcctta tcgccacccg ctgatgcgtc
    13861 agcgccagtc gctcttcctc caccagttta ttaaacgttt cgatcccgat tttctgaatc
    13921 agaaatttga tccgtgcctt gtgccggctg ttgcgttcac cataccggtc gaatacgcga
    13981 agtaccgctt cgagataggg gatcagtaag tcttcttcca ggaattcatg ggtgcagatg
    14041 gccaggtgtg gttgcgcacc cagtccgcca ccaatcagta ttttaaaacc tctttgttct
    14101 ttaccatcca ctgtcctcac ccgggcaatg gccccgagat catgcatgaa tgttaaggcc
    14161 gtatcttttt cggtattact aaatgcgatc ttgaatttgc ggcccatttc ctggcaaaga
    14221 ggtttacgca ggaagtattc aaacatcgca tgtgcatatg gcgtaacatc aaagggttct
    14281 tccggatcaa tacccgctgt atcggaggcc gtgatattcc tgacggtatt gccacaggct
    14341 tcccggatgg tgatgtcgtc tttatcaagt tcagcccata attgtggggt acggtccagg
    14401 ctcacaaaat ggatctgaat atcctggcgg gtggtgaggt gcagattgcc ggtggaatat
    14461 tcatcggata ccgcggcgat acgtttccat tgggccaggg tcatttttcc atagggtaat
    14521 ttaatacgca ccatttgtac gcccggctgc cgctggccat ataccccgcg ggcgaggcgg
    14581 agactgcgga atttttcttc atccattttt ccttcgcgga aaagaatgat ttttttttcg
    14641 agatcaagaa tatctttttc taccaatgga ttctctaact cagtgcggaa gctctgcata
    14701 tcgggtggtt attgtttacc cgccggttgg aacgaacggg ggatgaaaaa tactctacct
    14761 tataagtaga ctaacaaaat tacagggtcg cacccgtttg tcaaaaaata attatagcaa
    14821 taacagacgt acgtattaca gaataaaatg cgaaaaccct actaaattag taggaaatta
    14881 attactttcg tttttcgtcc cgtttatacc gggtttatat aaggcgtatg gagtaccagc
    14941 aattatttac cgatcagtta aaccgtttaa aagacgaagg cacctatcgc tattttctgg
    15001 aggtgaataa aagtgcgcaa cattttcccc ggtttaccta ccaggatgaa caggggaaca
    15061 gtcaatatgc cattaactgg tgcagcaatg attatctcgg actgagcagt gaagaaagcc
    15121 tgatttccaa actcggattt gcagcccatc gcagcggtac gggcagcgga ggtacgcgta
    15181 atatttccgg tactaccaat taccataaag aactggagcg cacactggcg gactggcatc
    15241 ggaaagaagg cgccttgctt tttaacgggg cctaccaggc caatgtcacc acgctgcaga
    15301 cactgggcag acagttaccc ggtcttgtct tcttttccga tgaacgcaac catgcttcca
    15361 tcattgaagg gatccggggt tgccgtaatg agaaaaagat ttttgcgcac aatgatatgg
    15421 agcaacttga agagctgttg aaacaagtgc ccgcagaaca acccaaactg atcgtttttg
    15481 aatcggtgta ctccatgagc gggacaattg caccggtaaa tgcgattatt gcacttgccc
    15541 ggaagtataa tgcactgacc tatgtggatg aagtacatgc cgtgggatta tatggcaaaa
    15601 caggtgccgg tatactcgaa gaggaaaatg tacagtcttc catcgatatc gtgaacggaa
    15661 ccctttccaa agctatcggg gtttttggcg gttatattgc cgcctcgtcg gtgctgattg
    15721 attttatccg cagtttcgga cgcggtttta ttttcactac ttcattgccg cctgcgatct
    15781 gtgccgcagc caacacgagt attcagctga ttcaacggga ggagcagcgg cgaagacaat
    15841 ttttttccaa tgtagcatac ctacgtcaat cattatacga aaatggggtc gactttaaac
    15901 tgaatccttc acatataacc ataataccgg ttgccggggc ggaccgttgc cgggaactgg
    15961 cccgtcagtt actggagaaa cagggtatct acctgcagcc gatcaattct cccacggtgc
    16021 cggttggaga agaatgcctc cgggtgatct gtaccgcccg tcatttaccc aaacatatcc
    16081 atcacctggc cttgagcctc aaaaaactat tgcatggaga ctatcagact gatcggcagg
    16141 aattcccgtc tttcgctctt gcagctggag attgtgaagg ccaggattga gcaggtattt
    16201 ccggaagtaa aagtggaact gattgcccgc agcagccgcg gtgatcagtt gtctggtatc
    16261 cccctgcata ctgtagaagg gcaggatttt tttacgcggg atattttcga ggccttgtca
    16321 acccatgaag ctgatatagc cgtgcactca ttgaaagata tgagtagtgc acattttttc
    16381 ggggatcatc tctttgccgt agtggaccgg gatgatacgc gcgacctcgc catcttcaac
    16441 ccgggcatac tggataaaat cagccgtggt gaaaccatta ttattggtac ctgttcgccc
    16501 cgccgcgaag aaatggccac ggtatttctg aaaaaagcat tgccgcaatt aaaggaaacc
    16561 attcatattg aaacgcgccc gatccgcggc aatgtcgaat cccgcttacg taagctggat
    16621 agcggagact atgatgcgac tatattggct acggcgggac ttaatcgctt gttacgtagt
    16681 ccggctgatt cggccatgat acaacagctg ctggccaata agaaattgat gctgttgccc
    16741 ctgattgaat gtgtaccggc gccttgccag ggagcgattg tggcggaagc acatccatcc
    16801 aatcaccggg cggccgatat actgaaagcg attaataatc ccatgctgct gcaggacgcc
    16861 attgcggaaa agcggacggc ggtgcaatat ggtacgggtt gtttacagca gtttggagtt
    16921 accactatca aaacgaagca aggagcatgt ctttttgcgg cgggtaaaga cgaccagagt
    16981 attgctttca cggactggca accatggccg gctttgccca ttgctcctgc tcatatattt
    17041 tccaccaccg accggatgcg tgattttttt aattatgaat ggcgggcggc gctgccggaa
    17101 ataaatttgc cggtggtgtt tatcgcgaat tataaattaa tggccggggt gatcaataag
    17161 aatgctttgc agaataaaac tatcctggtg tcaggcacca aaacctggta tgaattggcc
    17221 aggcaaggat actgggtaac tgctggtgct gatgccctgg ggtttgaata cctcttgcct
    17281 tcactctcta tgccactatt aaaaattgaa gccaacgata tcctgatact tacccatgaa
    17341 gccgcggctc agcgttggca aaagaaaggg tattccgccg cctgcaccta taaactgatc
    17401 cccgttttta atccgtcact ctttgaagaa atcgccggcg ccaaagccat ttgctggagc
    17461 agttttgccc agtatgatgc gtatggtcac ctcgcacaac ctggcattac acatatctgt
    17521 gcagggggag aaacagccac tcagttgaaa caggccggta tagatccggt gatttttcca
    17581 acgataaaag catttgaaca atggagaaaa ttgaataccc tctcacccaa cgccgcctga
    17641 ggacaagctc cctggtcagg gaactaaccg cctcagtaaa actgaatcac aaaagtttta
    17701 tcctgccact gtttgtggaa gaggggctgg ctgcaccacg accggtaccg ggattaggcc
    17761 ctgtgctggt agagaccata cattccatta ccggggtagt cgaaaagcaa ttagccaatg
    17821 gtattgccaa atttttatta tttcctgttc cggccggcaa gcaggagcaa ctgaaagatt
    17881 tttcatttgc tacgaacctc gttcgtacac tgaagcaaac ttttagcgaa cggatctgga
    17941 ttgcagctga tctttgtctt tgcagttata ctacacacgg gcactgcggt gtgctggatg
    18001 aggagcatac ccgtttactc aatagtccta cggtggatgt gttagtacag tatgcttcaa
    18061 aactggctgc ggccggtgtg gattgcatag cgcccagtga catgacggat ggccggattg
    18121 gcgcgatccg tacccggctc gatgcgcttg ggtttgaaga agtgacgatc atgagctatg
    18181 ccgccaagtt cagttcccag ttttacggtc ccttccggga tgcctgtcat tctgctcctc
    18241 aatcagcagg attacaaaac cgtaagacgt accagcattc accccttaat ccggcggatg
    18301 ccctggcctc tgcctcgcgg gatgaacggg aaggggccga tatcctgatg gtcaaaccgg
    18361 ccgcgttgta tacagatatt attgcttcgg taaaagcgag tactgagaag ccggtagccg
    18421 cctatcacgt aagtggtgaa tacgccgcca ttgaagcatt ggccgaaaaa gggttggtca
    18481 acagagcggc ggcgcacctg gaagtctgga ctgccctgag ccgtgccgga gcggatatca
    18541 tcatttccta tgctgcaggt gaagcccggc aatggattaa agaaatggag tattaatcaa
    18601 aaacccatag aaatggtaga ctggatgatt taaatcagct taagtttaca ttccggaccc
    18661 gagttttgcc cggttcgtgg aagtttaaaa attaccaaca gcattacaga caggattcat
    18721 ccatgcgaaa gaataaaaaa gaaataaagg agcaggtgcc tggcacaaaa aatccgcttt
    18781 acccggtttt cttaaaactg gatacattgg agctgttact ggtaggagcc gggaatgtgg
    18841 gattggagaa gttgcagtcc ctgttgggta atgcgccaca ggcccggatc accgtggtag
    18901 caccaaaaat tctggacgaa gtaagagtat tactggcaga acatccatcc tgtcagttga
    18961 aagaacgcga atttgtggaa gccgacctgg aacataaaga cctgctggta ctggccacgg
    19021 ataacccgga gctgcatcgg gaagtaaaac aaatcgccaa tgccaaaggt atactggtga
    19081 atgtagctga tacaccggag ctttgtgatt tttatctggg cagtattgtc caaaaaggaa
    19141 atctgaaaat cgccatttcc accaatggta aatcacccac ggctggtaag cggatcaaag
    19201 aagtgttgca ggatgcactg cctggtgaac tggatcaggt cattgaaaat cttcatattc
    19261 tgcgcggccg gttaaatgga aattttcagc ataaggttaa aaagctaaat aaaattaccc
    19321 gggtgatggt ggagaatgaa agttcgtcca ccgcccaacg atggcgcaag atcgccactt
    19381 attcgctgat tggatttgcc ctgatgctga tcgggcattt tattttttct tacattcctg
    19441 tacgtcagat cgccgatgat acatcggcct ggtataaaac actggatccg aattttcact
    19501 ggatggtgtt ggccgggttt atggcacaga tggtggatgg cgcaacgagt atgggatatg
    19561 gggtaaccag ttccatcgta ttgcagtcgg cccatgtaag cccggccgcc atcagcggcg
    19621 gaattcacac ggcagagatg ttcactaccg ctgcatctgg ttatagtcac tataaattcg
    19681 gaaatgtcaa taagaaatta tttaaggcac tggtgatccc tggaattatc ggggccatac
    19741 tgggcgcgtt tttgctggta aaattcgggg agacgcatat tcgtttcttg cggccggcga
    19801 tggccatcta taccttgctg ctgggtgtcc gcattttcag taatgctttc cgggccgtgg
    19861 atataaaaaa gaaattcaaa cgctttggct ggctggccgg tgccggtggt tttctggatt
    19921 cattcggcgg aggtggttgg ggaccgattg taacttccac actcattact aaaggacgaa
    19981 ctccacgcta tgtagtgggc tcggtaagtc tgaccgaatt ttttgtaaca ctcgccagtg
    20041 cccttacttt ctttacgatg ataggggtgc agaactggca ggtaatcctt gccctcgtat
    20101 tgggcggggt agtagcggcg cctatagcgg cccggctcgc gggtaaactg cccaaaaaaa
    20161 catccttcat tttcctgggc accgtggtgg tgctctggag tttcaggatt ttatataagg
    20221 cattctgagt agtaccagtt cgtttatttt atggtcatag cagatatttc tattgactag
    20281 aagcaggcag ttttactagc gctggtggtt aaccaattat aactatatcg tgcttcattg
    20341 ctactactat ttctttacat tcgtataata tgaatattgt aaggctatcg tttttttcat
    20401 tgctgtttta cgggtcctgt tttaacggcg aagcgcaaac caaacaaacc gaacaggcgg
    20461 agcaggtctg gctgggctac ctgaatcaga cccggctgag caataagtgg ggaatctggg
    20521 tggatgcaca tttgcggacc aaggacgatt ttgtatcgga gctcaacacc ggcatcttcc
    20581 ggacaggcct gacttattac atgaatgatg acctgaagtt gacagccggt tatgcatatg
    20641 tcaatcattt tccgggagat aatcataaaa atatttctca gccggaacac cggccctggc
    20701 agcaggttca atggcataca aaatatcccc gtttacgcct gatgcaatac ctgcgcctgg
    20761 aagaacgatg gcgaaggaaa gtgttaaacg ataatgaact ggcggacgga tatactttta
    20821 attttaaatt caggtataat tttctggcgc agtttccact ggggaaaatc aggttccagc
    20881 ctcgcagcct gtccctggta ctgaatgatg aagtgcatgt aaatttcggg aagcagatcg
    20941 tgtataatta ttttgaccag aaccgcttct ttaccgggtt gaattaccag ctcagtggaa
    21001 aggaaaatct gcaggtgggt tatctgtatg ttttccagca actgggagcc ggtaaccggt
    21061 ataaaaacat acatggtgcc cggatttttt atttccagaa cctggatctc cgtaaaaaga
    21121 aatgaactgt tttaagcaga actccgttta agaaaatcat ccatcagggg attaaaggct
    21181 tccggtgttt caagcatcgg cacatgcccg cattcatcga tccagtgcaa ttcagattgg
    21241 ggcatcagcc ggtgaaaact ctccgccact tcgggaggcg taatggtatc attctttccc
    21301 cagatcaggc agcagggcag ggtgagttta tgtaaatcct tttctacatt atgccggatc
    21361 gtggatttag ccagcgaaat gatctgaatg gccttgcggc tgttgaccgt agcataaatc
    21421 tcatccacca attccttagt cgccacttcc ggtttataaa aagtcagttc tgtcttggtc
    21481 ttgatatagt cgtaatcccg gcgtcttgga taggtatcgc ccatgccatg ttcatataaa
    21541 ccggaactgc cactcagggt cagggtcttt actttctccg gatgctggag cgtatacagt
    21601 aaggcaatat gcccgcccat ggagttaccc aacaggtgaa atttttccag ctgcagttca
    21661 tctgccactt cctgaacaaa tttcatcagg gtaaaaatat cgacacagaa tcccaattca
    21721 taaaaaggaa agatcggaac aatgacccgg tgagttttac gcaggtgttc aatcagcggc
    21781 tggaagtttt tcaccgaacc aaacagtcca tacaggagaa tgatcggatc tccctgtcct
    21841 tcatccgtga atcggggcgc agtatgtttg gcagaaaata atttcatatg taaacattat
    21901 cgcgaaaaac tacagttttg gttcgtttgc ccgatactgt aatcgataca gttccggttt
    21961 gggaagggtg actaccccat ccgggcggga aaataaggaa ataaccgttc gttaccaatg
    22021 attcagccag aattgaagag taattaaagc ccgggatcag cgatcacggg aatccccttg
    22081 tgtgggtcaa agggttatag cctattattt ttgtagacat cattttccca atcgattacc
    22141 ggtcccattg tatctttaca ggaatattaa agcagtactt caagatggca aaacaacaaa
    22201 aaccggctac ccgtgcagta agaatgcaga ctccaaaaac ggcgcagaaa gaacattcca
    22261 cgcctttgtt tctgaccagc agtttcacct atgattctgc ggaagatatg gccgcggcct
    22321 ttgccactga atcaccggaa gtcaatattt attcccgctt ttcgaatccg ggtgtggatg
    22381 agtttgtcaa taagatggcg gctcttgaag gagcggaaca tggggtagca accggtacag
    22441 gaatggccgc gatcttctct acgttcatga cctttctcag taagggggat catatcattt
    22501 ccgcatccgc cgtctttggg tccacgcata ccattctgac caaatacctg cccaaatggg
    22561 gaattgagtc ttcttatttc gatatgaatc aaccggagac gattgaaacg ttggtaaaac
    22621 ctgccacaaa aatgctttac ctggaaacgc cgagtaatcc ggggctggat attatcgata
    22681 ttgaatacgt tgctgcgatt tgtaaaaagc attctattat gctggtagtg gataattgtt
    22741 tcgccacacc agccgtacag caacccatac aatatggagc tgacctggtg atacattccg
    22801 ccaccaaatt tattgatggt cagggacgtg tattgggagg tgtgattgtc gggaaaaaag
    22861 aactggtaca ccagctctat ctttttatcc ggaataccgg accttccatg agtcccttta
    22921 acgcctgggt actgagcaag agcctggaga ccttattcat ccggatggac cgccacgcag
    22981 cttctgcact ggccattgct actaaactgc agggacatgc gaaaatcagc agggtgaaat
    23041 atccattcct tgaatcacat ccgcaatacg ccattgccaa aaaacaaatg agcaatggtg
    23101 ggggaattgt cacttttgaa ttgaagggag gagttgaaag tggccgtaaa tttctgaatg
    23161 cactggaaat gatttccatg accaataacc tgggcgacag ccgcaccatt gcctctcacc
    23221 cggcttccac cacccattcc aaattatcgg aagcggaacg tcagtcggtg ggtattaccc
    23281 caggtctgat ccgggtgtcc gttggactgg aagatgtgga ggatatcctg gatgatttat
    23341 tacaggccct ggataattcc tgattatttt ttctttcttt tggccagctg ctgtaaagta
    23401 gcccgcatgt ctttatgcag gggtgcttcg aggtcaacta tatttccatc tatatcggta
    23461 aaactcagct gaaacgcatg caaggccagc cggccgagga tgggacgttc ttccagttcg
    23521 ttcttggata atttgaattt agattttacg gcggataaaa gcagaggttt gccatctcca
    23581 tacaatgggt cgcatacaat cgggtagccg aggtctttca tatgtacccg gatctggtgc
    23641 gtacggccgg tgtgaatccg aaactgtacg tgtgagaaaa taccaaaatc agtcagtact
    23701 tcataatccg tgagcgcttc tttccctttg cgatgaacga tcatggtgcc gttcaacccg
    23761 ggatgctcca tcaacggggc ttcaatactg ccagcgggct gtgccggtga tccaatgacc
    23821 agtccctggt aaatcttttt ggtatgacgg tcctcgaatt gtttgcagag gtgtttatgc
    23881 gcggattcat ttttagcaaa tacgatcagt ccgctggtat cccggtcaat ccggtgcacc
    23941 gtgaatatct ccccgtactt ttctttcaat aatactttca gggatacttc ttttccttcc
    24001 cggtcaggaa tcgacaggag gccggacggc ttattgattg ccacccagtt ctccgtttca
    24061 gctataatgg agtcctttaa attcatatca gttaattggg aatggccggc tattttcctt
    24121 tgccaaaatg tttgagatcg cgtacttcct tttccgtcag gaaacgccac ttgccgcgat
    24181 ccacattctt cttggtgagt cctgcaaata ccacgcggtc caggttacgt acatcatagc
    24241 ccatcttttc aaagatgcgg cgtacgatcc ggttacgtcc gctgtggatt tccactccca
    24301 cctgtgattt gtctttggag tcagcatagg ccagcacatc cacattggcc ggtccgtcat
    24361 cgagggtcac cccattcagg atctgttcaa aatctctttt ctccaggggt ttattcaacg
    24421 taacgtgata tactttcttt acctcattgc tggggtgtgt gagtttctgg gccagttcac
    24481 catcattggt caggattaac acacctgatg tgtttcggtc cagccggcct accgggtaaa
    24541 ctctttcggt ggtagcccgt gcgattatat ccaataccgt tttccggccc tgcggatcat
    24601 cggtggtggt gataaaatct ttgggtttat tgagcagtat atagaccagg ttcttcgcca
    24661 ggaaaatctt tttcccgttg acccgtactt catctttggg cgatacttta aaaccgggtt
    24721 cattcacggt ctcgccattc acttttacaa gcccctgctt gaccagttca gcggcttcgc
    24781 ggcgggcagc cacaccggag tgggcgatgt atttattaag gggcatcagt tcgtcgctct
    24841 ctttccgctg atgggatact ggagccggtg cctggccttt cttgccggac tgctgattta
    24901 cattcggggt taaacgttga cgggcttctg ctttcttctg atcgaagaag gcttcccttt
    24961 cctttttgtc ttttcgtttt tcttgtttga attgttcttt gatcgcggcg ttgctcttct
    25021 ttttcgcgaa ttttccgaag ttgtttccgg gttgcttcat ggtgagtagt ttgctgtttt
    25081 acaacagtag tgagccgcaa aggtacggat ttccggccgg ttggccgaaa cttgcctgcg
    25141 gcaagatttt tgccacgaat tgccactaaa atgcacgaaa aatacataaa ggcaagccac
    25201 ggatcacacg gattaacacg gattggcccc ctaaatcccc cgaaggggga ctttgaagac
    25261 tcaaagaagt tagacaattc ttactcttag tcttgcgtaa aatttcccgc aaagctttat
    25321 aagacattc
//
LOCUS       DAOPMW010000015        22943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2062_length_22943_cov_9.947527, whole genome shotgun sequence.
ACCESSION   DAOPMW010000015 DAOPMW010000000
VERSION     DAOPMW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2062_length_22943_cov_9.947527"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLU11_01655"
     CDS             complement(<1..957)
                     /locus_tag="PLU11_01655"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN57838.1"
                     /translation="MLISRFAISQTCTIQCQNCIQSANGIQNDGSTGFCPGQTAKLYF
                     NGSCVTTLNQYTFQWQVSTGPGTWADINNATTDTLIVSIANTYQLVITGPGPCTSTCS
                     IIVNAYTSPIAEFSSVPNNTCGRTNVHFSNNSTLPGGGTMTYQWNFGDPGSGANNTSA
                     TTNPSHYYIPPIGTGTIIYTVTLIATSNTGCKDTVTHTVTIGERPDATLDEITTGGVV
                     TINGQLYFRKCSPTPWTMEFLNASTTNSFNTNYKIIWGDASPDFTSATFPGTVTHTYG
                     VGSYVLQYIVNSATCADTTRYNIFVGNQPAGGLLSPGSTTICN"
     gene            complement(1301..3688)
                     /locus_tag="PLU11_01660"
     CDS             complement(1301..3688)
                     /locus_tag="PLU11_01660"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN57839.1"
                     /translation="MRQFISCFLIVLATSTSLLAQNTYNNPNSNHGNKFEQMGGILPT
                     PNEYRTASGAPGPKYWQQRVDYDIKCELDEKNLKLNGSETVTYFNNSPNELVYLWLQM
                     DENQHSSVNNANYQTSNPMNRQLSPQAIDRVDEAKTDNGYGFNIVKLTDATGKSLKYT
                     VNKTMMRVELPVPLKAGQRFVFKLDWNYKIADRNGRGGRGGYEFFPEDGNHVFTMTQW
                     YPRLCVYSDFQGWQNHQFTGRGEFALTFGNFKVAMTVPADHVILSTGEGQNYAAVLSP
                     AQMARWNKAKNSTEPVEVVTLDEAKAAEKQKSDKKKTWIFKADNVRDFAWGSSRKFVW
                     DALATKIEGKKIMCMSGYPKEAYGLYRKYSSKAVEHTIKTYSKFTIPYPYPVAQSIEA
                     SNGMEYPMICFNFGRTEKDGTYSEGTKNGMLGVVIHEVGHNFFPMIINSDERQWSWMD
                     EGLNTFVEYLTEELWDNKFPSRRGPAAFITDYMKLPKDQLEPIMTNSENIVQFGPNAY
                     SKPATGLNILRETIMGREQFDYAFREYARRWAFKHPEPADFFRTMEDASGEDLDWFWR
                     GWFFSTEVCDISLDSVRYAKADLTAEPEKREASSFKRSADKPLLPTFDDISKIRNRED
                     KNIKFQTDADTTLRDFYWRYGRGLEPYDTSRKEVTMPAGGAEKLNEEEMQKYGNRHMY
                     ELSFSNKGGLVMPIIIEWTYKDGTKEIERIPAQVWRLNENKVVKSFIKDKEVASVKLD
                     PLKETADINEKNNSWNTIPEPSRFTIFKQAQGGGRRNQGGQGGNPMQRAQEKKGF"
     gene            complement(3822..6203)
                     /locus_tag="PLU11_01665"
     CDS             complement(3822..6203)
                     /locus_tag="PLU11_01665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN57840.1"
                     /translation="MNKSLLLSCMLLLMTGIAIAQIQNNASSNHANKFEQLGTILPTP
                     NEQRTASGAPGTKYWQQRVDYDIKCELDEANNKLTGSETITYFNNSPDVLTYFWMQLD
                     ENQHSSVNNAGYQTGNRMPQQTTDNMLDAMTEKKSDNGYGVNITKLTDALGKPLSYTI
                     NKTMMKVMLPAPLKPGQKFVFKCDWNYKIANRGDFIRFGGARGGYEHFAEDGNNNYTM
                     TQWYPRLCRYSDDQGWQNHQFTGTGEFTLCFGNFNVQMTVPADHIVGGTGECQNYAQT
                     LTPTQMARWKKAQTAKEPLQIVTLDEALQASKTKSKAKKTWIYKADNVRDFAWTSSRR
                     FVWDAMSSPIEGKKVMSMSYYAKEAYPIYSKFSTKAVAHTLKTYSHFTFPYPYPVAIS
                     VEGNQGMEYPMISFNPGRAEKDGSYSEGAKNAAILVIIHEVGHTFYPMIVNSDERQWT
                     WMDEGLNSYLQYLTQELWDNKFPSDGGPAFTITDYMKLPKDQLEPIMTNSENINNFGA
                     NAYDKVATGLNILRETIVGRELFDNAFREYSRRWAFKSPTPADLFRTLEDATGEDLDW
                     FWRGWFYSTDACDISIDSVKHAVPDVSAVPFLTKDTVVYRPLAKPQVNDFEDLSKIRN
                     RQDTTIKFETDADPSLRDFYWNYARGNAKYDTTKYPVTMRPQTETLDEAGKQKYGSKH
                     LYEVSFSNKGGLTMPIIIEWTYKDGTKEIERIPAQVWRKNEKNVIKTFLKDKEVASIK
                     LDPYKETADIDETNNSFGDFKEPSRFKVFKQKQNVTPAPGINPMQKAMEKKGF"
     gene            complement(6219..6797)
                     /locus_tag="PLU11_01670"
     CDS             complement(6219..6797)
                     /locus_tag="PLU11_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HPN57841.1"
                     /translation="MSDFKFYFGLGWEHIMSWDALDHLLFIAALAAIYLLKDWKQVLI
                     LVTAFTIGHSLTLALSVMDVFRFPAAWVEFLIPCTIVITAISNLFQKQFTAKSIRINY
                     FLALFFGLIHGLGFANTIRFMLARDQSMGWGLFGFNVGLEAGQIVVVATLLLVTQALI
                     TTFSINRREWVIFLSAAVFSLALQISLTRLPF"
     gene            6903..7418
                     /locus_tag="PLU11_01675"
     CDS             6903..7418
                     /locus_tag="PLU11_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57842.1"
                     /translation="MASSVYKWLLIPLICLLVAGKPAPHPFHVSVIEVNHNAADKTLE
                     ISCKIFTDDFEKILAKNYKTKVDLINPADKAPMDSLVKKYILSHLVISANGKPVSLHY
                     LGFENDKEAAYGYIEVENVPVLNKISFSTNLMYDQFEDQVNIMHVMVNGNRKSTKLNF
                     PETEAEIVFSP"
     gene            complement(7446..8747)
                     /locus_tag="PLU11_01680"
     CDS             complement(7446..8747)
                     /locus_tag="PLU11_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPN57843.1"
                     /translation="MDIFILSLLILLNGLFSMSEISLVSARKARLEHLAEKGDKKAIL
                     ALALSNHPELFLSAVQIGITLISIITGVYSGERFGRDLQPYVEQIALFKPYAETISTT
                     FIVILVTYLSIVFGELIPKRIGLLRSEKIAMMVAGPMKTFSRITHPFVWLLNKTSNIF
                     FSIFNIKRSKDDAVTEEEIKTLITEGTEAGTIDEAEQEIIERVFHLGDRNITSLMTHR
                     SDIVWFNLDDNEDKIKEKILQEPHSAYPICDGEIDNIKGVVSIKDLYISPDNTLFKEI
                     MQPALFIPENNTPYQVLEKFKESKIHSAFIVDEYGSILGLLTLNDILEAIVGDIQQQD
                     VPDYEIVEREDGSFIVDGQIPFYDFLTRFEKTEWMNEGEHDFDTLAGFVLHELERIPK
                     TGDKLSWKGFSLEIIDMDGHRIDKVLVTLSKELLEDIEESR"
     gene            complement(8747..9364)
                     /locus_tag="PLU11_01685"
     CDS             complement(8747..9364)
                     /locus_tag="PLU11_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPN57844.1"
                     /translation="MILPAINLLDKLVIWDKWLFVKINSQWTNPLFDNLLPYWRTAEF
                     WAPLYLFLLLFTLVNYKSKGAWWVLFFLCTFVACDLTGTHAFKKVFERVRPCNDPDFY
                     MQVRLLLDHCGSGFSFVSNHATNHFGMGIFGFVTLRHVIGKWAWLGPLWALSVAYSQV
                     YVGVHYPLDVMAGAALGTLFGISFGTLFNKRYGFAIFAHQSTVSV"
     gene            9487..11466
                     /locus_tag="PLU11_01690"
     CDS             9487..11466
                     /locus_tag="PLU11_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN57845.1"
                     /translation="MLARFRIPKTLLWVANLFGIFLLIFTGFRLATFLAFRPENFRFS
                     SALPAFGMGVRYDLRWISVILLPVVLLSSWPRLSPFFSSKNKKWWTWYLAIITFIVFF
                     FFAAGFGSFSYNRTPLDAGAMNFAEDFTISIKMIWQTYPLIWMLLGLVVAVLFFRWMY
                     HRSHWQVINRTDGKGIPHRRNFFFLSSLLLAILIYGSFSWPPLSRADSFRFNNSFTSY
                     LAINPLQNFVATLRLRKPDYNEKKAREVFPVMAEWMQLPNAAEFSFRREIGPRSTSFE
                     SRPNIILVQCESFSMYKSTMSGNPLNASPYFDSLCRQGIFFDRCFSPHFSTARALFAI
                     LSGIPDAQLFKFSTRNPEALQQHTIVNGLEGYDKHYFLGGDPEFNNFQGLLSNIRGLQ
                     MHTGDYFKTPKVNVWGVSDKDLFLAANDVFRKKDKPFFAYIQTSGNHRPYNKSIPETD
                     IDFIKVTIPDEEIKKYGFESLDEYNCFRYSDYCFRKFIEAAQKEEYFHNTIFVFVGDH
                     GLAGNASPMYPPVWTEQRLTDEHVPLLFYAPDLLAPQQRHEVVSQIDILPTIAGLLHQ
                     SYVNTTLGRDLLDPSKKNNFAFITNTSDRIGIVTDDFYLTRNINSGEEMLSPLLTGTT
                     TYTPAQKDSVHKRLSLFTTAFYETSKYLIMNNDRD"
     gene            complement(11526..12263)
                     /locus_tag="PLU11_01695"
     CDS             complement(11526..12263)
                     /locus_tag="PLU11_01695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN57846.1"
                     /translation="MTEQAWYKNWFNSSFYHKLYFKRDEKEAEAFINQLIQRLQPPAG
                     AKMLDVACGKGRHSKILAAKEYQVTGIDISPDSIRSAKKSEKENLEFFVHDMRLPFRS
                     NYFHYVFNFFTSFGYFRTRREHDAAFRTITSALLPGGQFVIDYLNVHYAEDHLVAAET
                     RQMENTLYDIHRWDDETHFYKKITVTDPSLKQPMSYTEKVAKFTLGDFTDMLSFQGLQ
                     VQEVFGDYSFSHYDVKKSPRLIIIARK"
     gene            12342..13721
                     /gene="glmM"
                     /locus_tag="PLU11_01700"
     CDS             12342..13721
                     /gene="glmM"
                     /locus_tag="PLU11_01700"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPN57847.1"
                     /translation="MALIKSISGIRGTIGGKPGENLTPVDIVKFAAAYGTIIAEGKER
                     AKIVIGRDGRISGAMVQDLVINTLTGLGIDVVDLGLSTTPTVEVAVKMANADGGIILT
                     ASHNPKEWNALKLLNEDGEFISAETGARVLELATKEEFVFASVDKLGTVTKDDSYGQL
                     HIDAILQYPLVNTAAIANQNFKVVVDAINSTGAIYVPALLKALGINDVIVLNGEVNGK
                     FAHNPEPLPEHLSQLCNEVVKQQADLGIAVDPDVDRLCFVNEDGTLFGEEYTLVAVAD
                     YVLSKRKGNTVSNMSSTKALKEVTLQHGGSYSPSAVGEVNVVKKMKETNAVIGGEGNG
                     GIIVPDFHYGRDALIGIALFLSHLADQKKGIKSLRGTYPDYFISKNKIELQNGVDVPG
                     IFEKIKVKYKNQPINTEDGLKIEFENDWVHLRTSNTEPIIRIYAESDFETKANNIAGQ
                     LMRDIREFS"
     gene            complement(14443..14889)
                     /gene="tnpA"
                     /locus_tag="PLU11_01705"
     CDS             complement(14443..14889)
                     /gene="tnpA"
                     /locus_tag="PLU11_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPN57848.1"
                     /translation="MSSYRQVYYHIVFGTKFREPAINPDHELDLFKYIWGVVKKNNCV
                     LHQLNGMPDHIHLLSDLHPSVSLSNFIKDIKVSSSIWLKDTGKFPLFTGWQEGYGAFS
                     CSDRDKERVIGYIKGQKAHHKSENFKEEYIRLLQEHRIKYDEKYLF"
     gene            complement(15414..17639)
                     /locus_tag="PLU11_01710"
     CDS             complement(15414..17639)
                     /locus_tag="PLU11_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPN57849.1"
                     /translation="MDQEYITNFLADEDDRVDGIDKVTGRAKYTADHHPENMVYGVFV
                     TSTIARGAIKNFKLNEAKDAPGVLEIITHLNCPALPGYNPWAKDPKKKGFEWRGLKPF
                     HQNLVYHYGQPIALVVATTWEKAVKAASLVKAEYINEVAETDFNSLRKTESKLKPSGE
                     YKRGQADAYKQAEVFVENEYYMPVETHNPIELHATIAQWDSEDKLLVYDKSQGPKGTQ
                     SELARLFGIEEKNVRVITKFLGGAFGSSLRSWPHVPAACIAAKMVKRPVRVQLTRRQM
                     FTMVGYRPMAWQKIAVGATKEGKLTGITHHAISNTSRYEDYREGIVNASHYLYECPNV
                     NTSYKILPLDVNTPTWMRGPGEATGAFALECILDELAYKLNMDPVELRIKNYAVLHPQ
                     SKLPWTSKFLRECYDKGREMIGWSNRPATPRSLTENGKLIGYGMGGGAFGASRGNATV
                     KAVFTSEGKLLLYSAVSDMGPGTATAMVSIAAQVMNMPSSKISFLLGDTEFPPGPTQG
                     GSTTTSTMGTAVSLVCDTLKAQFTEIALKQSPLFNGLSEADLEFDKESIRSKKETNKK
                     ISLTELLKASGKPQIEVLKESGPDPAVRSKYAMNSFSAHFIKVAVDPATGAIEFLKVV
                     CTGDGGKIVSPKTARSQMIGGAVGGIGMALTEEALIDKTTGKYLTDTLGSYQVPRHRD
                     VPFIHTWFPDLADPLINPMGSKGVGEIALIGFASALANAVYNATGKRLTHLPLKPEKL
                     V"
     gene            complement(17666..18280)
                     /locus_tag="PLU11_01715"
     CDS             complement(17666..18280)
                     /locus_tag="PLU11_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPN57850.1"
                     /translation="MEQHVHSCPGKSRRDFIKKSTALTAISLTPIAVVKAGENHEAIA
                     SFFEKTPLQMEVNGKTVQVLVDPGVSLLDMLREQLGLTGTKKGCDQGHCGACIVHVDG
                     KRVNSCLTEAVDNNGKKITTIEGLADGDKLHPVQEAFIKHDAFQCGYCTPGQIMSAVA
                     CIREGQANTETDIREYMSDNICRCGAYPNIVAAIREAKNSGQKI"
     gene            18437..19564
                     /locus_tag="PLU11_01720"
     CDS             18437..19564
                     /locus_tag="PLU11_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPN57851.1"
                     /translation="MNRIYLDNAATTPMDPQVLEAMLPYMTSHFGNPSSVYSYGRETR
                     LAIENARKTVARILQANPGEIFFTSGGTESDNMAIASAIHDLGCRHIITSAIEHHAVL
                     HTVEHYDHRDEVTISFVKLLSDGHIDLEDLEAQLAARPERCLVSLMHANNEIGNLLDI
                     YATGEICKKYNAIFHSDTVQTIGHYKIDLRKNPVHFATAASHKFHGPKGVGIIYINDN
                     IKINPIVFGGGQERNMRAGTENLYGIVGFAKALELADASHEKDSTYIQSLKQYMIEQL
                     EQHIPDVAFNGDYKGQCLYTVLNVSFPKTEKSEMILFSLDMQGVCASGGSACSSGADI
                     GSHVIRAINNNPNRVPVRFSFSKYNTKEEVDVVVEKLKQLI"
     gene            19923..20279
                     /locus_tag="PLU11_01725"
     CDS             19923..20279
                     /locus_tag="PLU11_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPN57852.1"
                     /translation="MEKLTHAEEETMQAVWRTGEGNVKAFMEYLDNDIPYTTVASTIK
                     NLEKKGYLTSRLLGNAYLYHPAVSEEEYKEKFMGKVVKEYFDNSYKELVNFFVEQKKL
                     SARELKEIIQLIEQGK"
     gene            20344..22317
                     /locus_tag="PLU11_01730"
     CDS             20344..22317
                     /locus_tag="PLU11_01730"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPN57853.1"
                     /translation="MPFIFSYLLKLSISLSVVFLFYHFVLRRLTFYTWNRWYLIGYSV
                     LCFFLPFVDISFALEQSDIKNSPVVKWVPVLGQRIGLDTGADSGPVSGWEYALLVLMA
                     GVGVMLVRLGIQLISIYRMRKRSSMISQDGIRIYQVDAPIIPFSFGDSIFINQHQHSL
                     EELEEIIRHEFVHVKQKHSVDIIWGELLCLLNWYNPFAWLLKKSIRQNLEFIADRHVL
                     EQGMDRKQYQYLLLKVIGNNHFSIANQFNFSSLKKRIAMMNKLRSAKFHLVKFMFVLP
                     LVSVMLLAFRNEWKKQEPRTEQLMTAVIAGNDTIPDKLPPPPPPPPPPPKGQGTIKDF
                     IDRNQGVKNLGWVYHHDNSVAMIHVMKKDGATEIYHTDNAEEMNAGAKKYGKFPVPPG
                     AMAPPPPPGQDKHVWVQGYPIAPVDPKDVVVVQGYPIAPPAPASGVVVEGYPIAPVPP
                     HGEIVVTGYARPPAPVKLPDHVKSISIKNGDKAVVVLKNGKTELYDLNNPEQKKKFDA
                     QYPAPPPPPPPPAKGVRVEGRPLNGSVGATVVEGKPLPDNLIYVIDGIITTKFEMEKI
                     SPDKIASIDILKGESAVALYGENAKEGVVQIKLKYEAQPLYVINGEISSRETMDKLKP
                     EQIESVDVLKGEKATAAYGDKGKNGVIMIKTKK"
     gene            complement(22368..22589)
                     /locus_tag="PLU11_01735"
     CDS             complement(22368..22589)
                     /locus_tag="PLU11_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HPN57854.1"
                     /translation="MNRFKDFVEWNAFGVCSAIGNRLGIATSRIRQYFMYASVLTMGS
                     PIIIYMVLAFFRNVRKYVMAARRNPWYYL"
BASE COUNT         5969 a         5057 c         5494 g         6423 t
ORIGIN      
        1 attacagata gtggtactgc ccggacttaa aagtcctccc gctggctgat taccaacgaa
       61 aatattataa cgggtggtat cggcacaggt ggccgagttc acaatatact gaagtacata
      121 cgagcctact ccataggtat gtgtaacagt acccgggaag gtggcgctgg taaaatcagg
      181 agaggcatcg ccccagatga tcttataatt ggtattgaaa ctgttggtgg tggatgcatt
      241 gagaaattcc atggtccagg gagtgggcga acatttccgg aagtacaact ggccattgat
      301 ggtgaccaca ccaccggtcg tgatttcatc gagcgtggca tccggtcttt caccaattgt
      361 aaccgtgtgg gttaccgtat ccttacagcc ggtgttgctc gtggcaatca gggttactgt
      421 ataaatgatt gtcccggtac caatcggcgg gatataataa tgactgggat tagttgtggc
      481 ggatgtgttg tttgcaccag aacccggatc tccaaaattc cattgatagg tcatagtgcc
      541 gccgccgggg agcgtggagt tattactaaa atgtacattg gtccggccac aggtattatt
      601 aggaacggaa ctgaattctg caattgggct agtgtaagca ttaactataa tactacaagt
      661 actggtacaa gggccaggtc cggtaattac cagctgataa gtgtttgcaa tagaaacaat
      721 cagtgtatcg gtagtggcgt tatttatatc agcccaggta cccggaccag ttgatacctg
      781 ccattgaaag gtatactggt tgagcgtagt gacacaggaa ccgttaaaat agagttttgc
      841 ggtctggccg ggacagaatc cggtgcttcc atcattttga atgccattgg cggattggat
      901 acaattctga cactgaatgg tacaggtttg actgattgcg aaccggctga taagcataaa
      961 gcagactaaa aggatggata cctttatgct tacagatgtt ctgttcggga gaaagaggtg
     1021 gtggccggaa atcatattcg gttcaaaagc aatgagcaat aaggtttaaa ctggttgcga
     1081 aaaatagaaa ataggaccgg cacctgcaat cttttacgat tgtattttag gagattcccc
     1141 tgttattttt acgaaaaggg tgttttcccc cttcttttgg tcctgataat gccagttatc
     1201 ggaaaactac gtatcgccgg attggctttt taaggattgc tgaaataaaa aaacctccgg
     1261 tttgtaccgg aggttttcat tattattttt tgaaacagga tcagaatcct ttcttttcct
     1321 gggcacgctg catcgggttt cccccttgtc cgccctggtt tcttcttcct ccgccctgtg
     1381 cctgtttgaa aatcgtaaag cgggaaggtt ccggaatggt attccagcta ttgttttttt
     1441 cgttgatatc ggctgtttct ttcaacggat cgagttttac ggatgccact tctttatcct
     1501 tgataaaact tttcaccact ttattttcat tgagccgcca tacctgggca ggaatgcgtt
     1561 caatttcttt cgtaccgtct ttatacgtcc attcgataat gattggcatc accagtccgc
     1621 ccttgttact gaaagagagt tcatacatat gacggttgcc gtatttctgc atttcttctt
     1681 cattcagttt ttcagcacct ccggctggca tagttacttc tttacggctg gtatcatacg
     1741 gctcaagtcc gcggccatag cgccagtaaa aatcacgcag tgtggtatcg gcatcagtct
     1801 ggaactttat attcttatct tcccggttgc ggattttaga gatatcatca aaagtgggca
     1861 acaggggttt atccgcactc cgtttaaaag aggaagcttc gcgtttttcc ggttccgcgg
     1921 ttaaatctgc tttggcatat cggacagaat ccagtgaaat atcgcaaact tcagtactga
     1981 aaaaccagcc tctccagaac caatccagat cttcgccgct tgcatcttcc atcgttcgga
     2041 agaagtcggc cggctcaggg tgcttgaagg cccagcgacg tgcatattca cggaaggcat
     2101 aatcaaattg ttcccggccc atgatggtct cgcgcagaat attcagaccg gtggcgggtt
     2161 tggaataagc gttgggacca aactgaacaa tattttcact gttggtcatg atcggttcca
     2221 gctgatcttt gggaagtttc atgtaatctg tgataaaagc tgcaggaccg cggcgactgg
     2281 ggaatttatt atcccacagt tcttcagtga ggtattctac aaaggtgttc agtccttcat
     2341 ccatccagct ccactggcgc tcatcactgt tgatgatcat cgggaagaag ttgtgaccca
     2401 cttcgtggat gaccacgccc agcatcccgt tcttggtgcc ttcgctgtaa gtaccgtctt
     2461 tttcagtacg gccgaagttg aagcagatca tcgggtattc catcccgtta gaagcttcaa
     2521 tactctgggc gaccgggtat ggataaggga tcgtgaattt ggaataagtt ttaatcgtat
     2581 gttctacggc tttggaggag tatttacggt aaagaccgta agcctctttt gggtagccgc
     2641 tcatacacat gatctttttg ccttcgattt tggtagccag cgcgtcccaa acaaatttgc
     2701 gggaagatcc ccaggcaaag tcacgtacat tatccgcttt gaaaatccag gttttctttt
     2761 tgtcgctttt ttgtttttca gcggccttgg cttcatcgag tgtaaccact tcaaccggct
     2821 ctgtggagtt cttcgctttg ttccagcggg ccatttgagc aggggataat acagccgcat
     2881 aattctgtcc ttcgccggta gacaggatca cgtgatcagc aggtactgtc atcgccactt
     2941 tgaaattgcc aaaagtcaga gcgaattcac cacgtccggt gaattgatga ttctgccagc
     3001 cttggaaatc gctgtataca caaagacggg ggtaccattg ggtcatagtg aatacatggt
     3061 tgccatcttc ggggaaaaat tcatatccgc cacgtccgcc ccggccattc cggtcagcaa
     3121 ttttataatt ccagtccagt ttaaatacaa aacgctgacc ggctttcagt ggaacaggca
     3181 gttccacccg catcatggtt ttattgacgg tgtatttaag ggatttacca gtggcatcgg
     3241 tcagtttaac tatgttgaaa ccatacccat tatccgtttt tgcttcatcc acacggtcaa
     3301 tggcctgggg actgagctga cggttcatcg gattactggt ctggtaattg gcattattca
     3361 cactgctgtg ctggttctcg tccatttgca gccagagata cactaattca ttaggggagt
     3421 tattgaaata agttacggtt tcactgccgt tcagtttcag gtttttttca tcgagttcac
     3481 atttgatatc atagtccacc cgttgctgcc agtattttgg accgggggca ccactggctg
     3541 tccggtattc attcggtgtg ggtagaatac ctcccatttg ttcgaattta ttaccgtgat
     3601 tgctgttggg gttattgtac gtgttttgtg ccagcagact ggtgctggtg gccagtacaa
     3661 tcaaaaagca ggaaatgaac tgtctcattt gatatgttga tttttaataa tggattttgt
     3721 tccggaatca ctaaagtaag gaattcaggt gtatgccggt tcagtccata aaaagaaaaa
     3781 gccgctattt gataagcggc tttttctcat ataagaagaa atcagaatcc tttcttctcc
     3841 atggctttct gcatcgggtt gatgccgggg gccggagtta cattctgttt ttgcttaaat
     3901 actttgaaac gggaaggttc tttgaaatca ccgaaactgt tattcgtttc gtcgatatct
     3961 gccgtttctt tgtacgggtc cagtttaata gaagccactt ctttgtcctt taaaaaggtt
     4021 ttgatgacat tcttttcgtt tttgcgccat acctgggcag gaatacgttc aatttctttg
     4081 gtgccatctt tatacgtcca ttcaataatg attggcatgg tcaggccgcc tttattgctg
     4141 aaagacactt cgtataaatg tttgctgccg tatttctgtt taccggcttc gtccagcgtt
     4201 tctgtctgtg gtcgcatggt taccgggtat tttgtagtgt catatttggc attaccgcgg
     4261 gcatagttcc agtaaaaatc acgcaaagaa ggatccgcat cggtctcaaa ttttatagtt
     4321 gtatcctggc ggttacggat tttggacagg tcttcaaaat cattgacctg aggttttgcc
     4381 agcgggcgat acaccacggt atctttggtc agaaagggta cggctgaaac atccggtacc
     4441 gcatgtttca cggaatcgat agagatgtca caagcatccg tactgtagaa ccatccgcgc
     4501 cagaaccaat ccaggtcttc cccggtggca tcttccagtg tacggaacag gtcagccggg
     4561 gtaggggatt tgaaggccca gcgacgtgaa tattcacgaa aagcattgtc aaacagttcg
     4621 cggcccacaa tggtctcccg gaggatattc agtccggtcg ctactttatc gtaggcattg
     4681 gcgccgaaat tattgatgtt ttcactgttg gtcatgatgg gctccaactg gtctttcggc
     4741 aacttcatat agtcggtgat agtgaatgcg ggaccgccat cagaagggaa tttattgtcc
     4801 cataattcct gggtcaggta ttgaaggtag gagttcagtc cttcgtccat ccaggtccac
     4861 tggcgttcgt cgctgtttac gatcatcggg taaaaagtat gtcctacttc atgtataatc
     4921 accaggatcg ctgcgttctt ggcaccttca ctgtaactgc catctttttc tgcacggccg
     4981 gggttgaagg agatcatcgg gtattccata ccctggtttc cttccacact gattgctacc
     5041 gggtaagggt aagggaaagt gaaatgcgaa taagtcttta acgtatgcgc aacggccttc
     5101 gtggaaaatt tactatagat cggataggct tccttggcat aatagctcat ggacattacc
     5161 ttttttcctt caattggtga tgacatcgca tcccatacaa aacggcggga agaggtccag
     5221 gcgaagtcac gtacattatc cgctttatag atccaggttt ttttggcctt gctctttgtt
     5281 ttggaagcct gtaatgcctc atcgagtgta acgatctgca ggggctcttt ggcggtttgt
     5341 gcttttttcc accgggccat ttgagtgggc gttaatgttt gcgcataatt ctggcattct
     5401 ccggtacccc ccacaatatg gtcggcaggt acggtcatct gtacgttgaa atttccaaag
     5461 cagagtgtga actcaccggt accggtaaac tggtggttct gccagccctg gtcatcgctg
     5521 taccggcaga gacgtggata ccattgggtc atggtatagt tattattccc gtcttcggca
     5581 aaatgttcat agccgccacg ggcaccgcca aaacggataa aatctccgcg attggcgatc
     5641 ttataattcc agtcacattt aaaaacgaat ttctgaccgg gcttcagggg cgcgggcagc
     5701 atcactttca tcatcgtttt gttgatggtg taactgagtg gtttgccgag cgcatcggtg
     5761 agtttggtga tattcacacc atacccgtta tccgattttt tttcagtcat cgcatccagc
     5821 atattgtcgg tggtttgctg gggcatccgg ttgccggtct ggtaaccggc attgtttacg
     5881 gagctgtgct ggttttcgtc cagctgcatc cagaaatacg tcagtacatc aggtgaattg
     5941 ttgaaataag tgatagtttc cgaaccggtc agcttgttat tcgcttcatc cagttcgcac
     6001 ttaatatcat agtctacccg ctgctgccag tatttggtac cgggggcacc actggcagta
     6061 cgttgttcat tcggggtggg cagaatagtg cccagttgct caaatttgtt ggcatggttg
     6121 gagctggcat tgttctgaat ctgggcaata gcaatgccgg tcatcaacag cagcatacaa
     6181 ctcagtaaaa ggcttttgtt catgtagagg tatatttatt aaaaaggtaa acgtgtaagt
     6241 gaaatctgaa gggcaagact aaaaacggca gctgaaagaa agatcaccca ttcccggcgg
     6301 ttgatgctga aagtggtgat caatgcctgg gtgacaagca ggagggtggc aacgaccacg
     6361 atctgcccgg cttccaatcc tacattgaag ccgaaaagac cccagcccat gctctggtcc
     6421 cgggccagca tgaagcggat ggtattggca aaccccaacc catggatcag tccaaagaat
     6481 aaggccagaa aataattgat ccgtatggat tttgccgtga actgcttctg aaacaggttg
     6541 ctgatagcgg ttatgacgat cgtacaggga atcaggaatt caacccatgc tgcggggaaa
     6601 cggaaaacat ccatgacgct cagggccagg gtcagggaat gcccgattgt aaaggcggtg
     6661 accaggatca ggacctgttt ccagtccttc agcaggtaaa tggctgcgag ggcggcaatg
     6721 aagagtaaat ggtccagggc atcccagctc atgatatgct cccaccccag tccgaaataa
     6781 aatttaaaat cactcatcct tttttgggtt tatacatcga accaatcgac tatttttacc
     6841 gtccttgctt cgggccaaca aaataagccc aagttgagta atcctgaaaa ataatagtgt
     6901 caatggcatc atccgtctat aaatggcttt taatcccgct gatttgcctg ttggtggccg
     6961 gaaaaccggc cccacacccg ttccatgtaa gtgtgataga agtaaatcac aatgcggccg
     7021 ataagaccct ggagatcagt tgtaagattt ttaccgatga tttcgagaaa attctggcca
     7081 agaactacaa gacgaaagtg gacctgatta acccggcaga caaagccccg atggattcgc
     7141 tggtaaaaaa gtatatcctc agtcatctgg taatatccgc caacgggaaa ccggtcagtc
     7201 tgcattatct cgggtttgaa aatgacaagg aagcggctta tggctatatc gaagtggaga
     7261 atgtaccggt tttgaataag atcagctttt ccaccaacct gatgtatgat cagtttgaag
     7321 accaggtgaa tatcatgcat gtgatggtga atggtaaccg gaagagtacg aaactgaatt
     7381 ttccggaaac agaagcggag atagttttta gtccatagtc gttagtaggc actattgact
     7441 aatgactatc gactttcctc tatatcttcc agcaactcct tactcaacgt caccagcact
     7501 ttatcaatcc ggtgaccatc catatcaatg atctcaaggg agaagccttt ccagctgagt
     7561 ttgtcccctg ttttgggtat cctttcgagt tcatgtagta caaatccggc cagggtgtca
     7621 aagtcgtgtt ccccttcatt catccattcc gttttttcaa agcgggtcag gaagtcatag
     7681 aaaggaatct gtccgtctac gataaagcta ccatcctctc tttccacaat ttcgtaatcc
     7741 ggtacatcct gctgttgaat atcaccaacg atggcttcca ggatatcatt taatgtaagg
     7801 aggcccagta tacttccgta ttcatcaaca ataaaagcag aatgtatttt tgattctttg
     7861 aatttttcta atacctggta aggggtgttg ttttccggga tgaacagggc cggttgcata
     7921 atttccttaa acaaggtatt atccggactg atataaagat ccttgatgga aaccactcct
     7981 ttgatattgt ctatttctcc atcacagatc gggtaagcag aatggggttc ctgcaggatt
     8041 ttttccttga ttttgtcttc attatcatcg aggttgaacc agacgatatc actccggtgc
     8101 gtcattaaag aagtgatatt ccggtcgccc aggtgaaaga cccgctcaat aatctcttgc
     8161 tctgcctcat caatggtacc ggcttcggtg ccttcggtga tcagggtttt aatttcttct
     8221 tccgttaccg catcatcttt tgaacgtttg atattaaaaa tggagaagaa gatattgctg
     8281 gttttattaa gcaaccatac aaaggggtgc gtaatccggg aaaaggtctt catggggccg
     8341 gccaccatca tggcgatctt ctcagagcga agcagtccga tccgtttggg aatcagttct
     8401 ccaaatacga tggaaagata agtgaccagg ataacaataa atgtggtgga aatagtttct
     8461 gcgtagggct taaataaggc aatctgctct acataaggtt gcaggtctct gccaaaccgt
     8521 tcaccggagt acacacccgt aatgatggat atcagggtga tacctatctg aaccgctgat
     8581 aaaaacagtt cgggatgatt ggagagggcc agggcgagga tggccttttt atcccctttt
     8641 tctgccaggt gttccaacct ggccttccgg gccgaaacca gggaaatttc cgacatagaa
     8701 aaaaggccat taagcagaat caggagactg agtataaata tgtccattaa acggaaaccg
     8761 ttgattgatg ggcgaagata gcgaatccgt agcgcttatt aaacagggtc ccgaagctga
     8821 tgccaaagag ggtgccaagg gcagctccgg ccatgacatc cagcggataa tgcactccga
     8881 cataaacctg ggaataggct accgataaag cccagagcgg gccaagccag gcccatttgc
     8941 cgattacgtg gcggagggtg acaaaaccga atatgcccat gccaaagtgg ttagtggcgt
     9001 gattcgaaac aaaactaaaa ccggaaccgc agtgatccag caacagcctt acctgcatat
     9061 aaaaatcagg atcattgcag gggcgcaccc gttcaaatac ctttttgaaa gcatgcgtgc
     9121 cggtaaggtc gcaggccaca aacgtgcata gaaagaacag aacccaccag gctcctttgg
     9181 atttataatt gaccagtgtg aataacagca aaaacagata aaggggtgcc cagaattcag
     9241 ccgttctcca gtaagggagc aggttgtcga ataagggatt cgtccactgg ctgtttattt
     9301 ttacaaacag ccatttatcc catattacca gtttatccag caggttgatc gcaggcagta
     9361 tcatccctgc aaatgtagga tttgcgggta tataccctga tacagtgttc aaataagtga
     9421 taatgggtat ggtgataagc cgatttagta ttttctttgc agcacactaa tacgataacc
     9481 tcttacatgc tggcgagatt ccggatacca aaaaccttac tgtgggtcgc caacctgttt
     9541 gggatttttc tgttgatttt tacgggtttc aggctggcga ctttcctggc ctttcgtcct
     9601 gaaaatttcc gtttttcatc agcccttccc gccttcggga tgggggtccg gtatgatctc
     9661 cgctggattt cggtgatcct gctgccagtg gtattgctca gcagctggcc gcgattgtcc
     9721 ccttttttct cttccaagaa taagaagtgg tggacctggt acctcgccat tatcactttt
     9781 atcgttttct tcttttttgc tgccgggttt ggcagtttct cctataaccg tacgccgctg
     9841 gatgccgggg ccatgaactt tgcggaagat tttactattt ccattaaaat gatctggcag
     9901 acctacccgc tgatctggat gctgctggga cttgtggtgg ccgttctttt tttccggtgg
     9961 atgtaccatc gcagccactg gcaggtgatc aatcggaccg atggcaaagg gattcctcac
    10021 cggcgtaatt ttttcttcct cagcagcctg ctcctggcta tccttattta tggttcattc
    10081 agctggccgc ccctgagccg ggcagacagt ttccggttta ataacagttt tacttcctac
    10141 ctggccatta atcccttaca aaatttcgtc gccacccttc gtttgcgcaa accggattat
    10201 aatgaaaaga aagccaggga ggtattcccg gtaatggcag aatggatgca gttacccaat
    10261 gcggctgaat tcagtttccg tcgcgagatc ggcccccgga gtacttcatt tgagagccgc
    10321 ccgaatatta tattggtgca atgtgaatca ttcagcatgt acaaaagcac catgagtggc
    10381 aacccgctca atgcctctcc ttattttgat tccctttgcc ggcagggtat attttttgac
    10441 cgttgttttt ctccccattt ctccaccgcg agagcgttgt ttgccatcct ctcaggaatt
    10501 ccggacgcac agctattcaa gttttccacc cggaaccctg aagccttgca acaacatacc
    10561 attgttaacg gcctggaggg ttatgataaa cattatttcc tgggtgggga tccggaattc
    10621 aacaattttc aaggcctgct gagcaatata cgcgggctgc agatgcatac cggggattat
    10681 tttaaaacac caaaagtaaa tgtgtggggg gttagtgata aagatctgtt cctggcggcg
    10741 aatgacgtgt tccggaaaaa ggataagccc ttttttgctt atatacagac ttcagggaat
    10801 catcgcccat ataacaagag cattccggaa acggatatag attttataaa agtgacgatt
    10861 ccggatgaag aaataaaaaa atacggattt gagtcactgg acgaatacaa ttgtttccgc
    10921 tactctgatt attgtttccg gaaatttatt gaagccgcgc aaaaggagga atattttcac
    10981 aataccatct ttgtatttgt cggtgatcat ggtctcgcgg gaaatgccag tccgatgtat
    11041 ccacctgtct ggactgaaca acggctgaca gatgagcatg tacccctgtt gttttatgca
    11101 ccggacctgc tggcgcccca gcaaagacat gaagtagttt cacagattga tatcctgccc
    11161 acgattgccg gcttactgca tcaatcttat gtcaatacca ccctcggccg tgatctgttg
    11221 gatccgtcca agaaaaataa ttttgctttt attacgaata cttctgaccg gatcggaatc
    11281 gtgacggatg atttttacct gacccgaaat attaattcag gagaagagat gttgtctccg
    11341 ttactgaccg gcacgacgac gtatactccc gcccaaaagg actcggtcca caaaaggctt
    11401 tctttgttta cgactgcttt ctacgaaaca tcaaagtacc ttatcatgaa taatgaccgg
    11461 gactgatttg ctctttagcg atggaggagg caggaactga attcttgtat tttactttcc
    11521 gataattatt tccgggcaat gatgatcagc cggggtgatt ttttcacatc ataatgacta
    11581 aaggaataat ccccaaacac ttcctgtacc tgcagtccct ggaagctaag catatcggta
    11641 aaatcgccta aggtaaactt ggccactttc tccgtatagg acatgggttg ttttaatgag
    11701 ggatcggtga cggtgatttt tttgtaaaaa tgagtctcgt catcccatcg gtgaatatca
    11761 tacaatgtgt tttccatctg ccgggtctcg gcagccacca ggtgatcttc tgcataatgt
    11821 acattcaggt agtcgattac aaattgtccg ccgggcagca gcgcggaagt aatggtgcgg
    11881 aaagctgcat catgttcgcg gcgggtgcgg aaatatccaa agctggtaaa aaaattaaac
    11941 acataatgaa agtaattgct gcgaaagggg agtcgcatat catgcacaaa aaattcaagg
    12001 ttttcttttt ccgatttttt tgccgaccgg atactatcag gggagatatc aatcccggta
    12061 acctggtatt cttttgccgc caggatttta ctatgtcgcc ctttaccaca ggccacatcg
    12121 agcattttag caccggcagg tggttgtaag cgctggatca gctgattgat aaaagcttcc
    12181 gcctcttttt catccctttt aaaatagagt ttatggtaaa aggaagaatt aaaccagttt
    12241 ttgtaccaag cctgctccgt catatggcaa atgtaagccg gccggttaaa ataaccgatt
    12301 gaatctgaaa tgcgtatttt tgcagcctta aaaaaaatca agtggcacta atcaagagta
    12361 tttcaggaat caggggaacg ataggcggga aaccgggcga aaatctcacg ccggtggata
    12421 ttgtgaagtt tgctgctgct tacggaacaa ttatagctga agggaaggag cgggcaaaga
    12481 ttgtaatcgg ccgtgacggg cggatcagtg gggcgatggt acaggacctg gtaatcaaca
    12541 ccctgaccgg attagggata gatgtggtgg acctgggact ttccaccacc ccgacggtgg
    12601 aagtggcggt gaagatggcc aatgcagatg gcggaatcat tctgacggcc agtcataatc
    12661 ccaaagaatg gaacgcgctc aaattactga atgaggacgg tgaatttata tctgcggaaa
    12721 ccggtgcccg cgtactggaa ctggccacaa aggaagaatt tgtatttgca tcagtcgata
    12781 aactgggtac ggtaacaaag gatgacagtt atggtcagct ccatattgac gccattctcc
    12841 aatacccgct ggtgaatact gcggccatcg ccaatcagaa ctttaaagtg gtggtggatg
    12901 ccatcaattc taccggggcc atttatgtac ctgccttgtt gaaggcattg gggataaatg
    12961 acgtgattgt gttaaacggg gaagtcaatg gaaaatttgc ccataatccg gagccactac
    13021 ctgaacacct ctcccaactg tgtaatgagg tggtgaagca acaagccgac ctcggtattg
    13081 ccgtggatcc ggatgtggac cggctatgtt ttgtgaatga ggacggaact ttatttggag
    13141 aagaatatac attggtggcc gtggcagact atgtgctgag taagcgtaaa gggaatacgg
    13201 tcagtaatat gagcagtacc aaggcgctga aagaagtaac actgcaacat ggcggttcct
    13261 actcaccgtc agcggtgggt gaagtgaatg tggtgaagaa aatgaaggaa acgaatgcgg
    13321 tgattggtgg cgaaggaaat ggtggcatca tcgttccgga ttttcattat ggtcgtgatg
    13381 ccctgattgg tatcgcatta ttcctgagtc acctggctga tcagaaaaaa gggattaaat
    13441 cattaagggg tacttatccg gattatttta tttccaagaa taagattgag ttgcagaacg
    13501 gcgtggatgt gccggggata tttgagaaaa taaaagtaaa atacaagaac cagcccatta
    13561 ataccgaaga cggattaaag atcgaatttg aaaatgactg ggtgcacctg cgcacctcga
    13621 ataccgaacc catcatccgc atttatgcgg aaagtgattt cgaaaccaag gccaataata
    13681 ttgccggtca gctgatgcgg gatataaggg agtttagcta agtcaaataa atttttcaga
    13741 aaagctgttt ccgtatttga aacagctttt tttatgggtt tcatggaatt agggaggcat
    13801 aaaacaccgg tttcaccggt tgttaaaatt attcaacccc gttggggttg ggggctgtgg
    13861 gtgcctttta ccgccagttt cactggcggt tatcgttgtt caacccctat cggggttggt
    13921 tgattgtcaa actattcaga tgggatttag ttgttatgtc aggatcactg gctggcagat
    13981 aaatttattt agttccggat tctttttcac gctacatcgg agatatcggg gaggttgata
    14041 gttacaatat tgtagatgca ctgcctgaaa tacaaatctt ctcaacccca acaaggttga
    14101 ataaagtgag tcctaaatta atttaaccca ttttttttac acactataaa ggatatacgg
    14161 ggtgcttcct atttataatt ttgtaaacgt tctgactgaa ttggattttt tccaacccca
    14221 acggggttga ataatgtgag ctcgtaaatt aatttagccc aattttttac acgccataaa
    14281 ggatatacgt ggtgcttcct atttatattt ttataaacgt tctgactgaa ttggattttt
    14341 tccaacccca acggggttga ataatgatag cccctggtga aaccaggggt ataatgatta
    14401 gagagccccc cacaaccccg aatggggttg aactaaaatc tgctagaaaa gatatttttc
    14461 atcatattta atccggtgtt cctgtaacaa ccggatatac tcttctttga agttctccga
    14521 tttatgatgt gctttttgcc ctttaatata gccgatcact ctttctttat cacggtctga
    14581 acaactaaag gcgccatatc cttcctgcca accggtgaat aaagggaatt tgccggtgtc
    14641 tttcagccaa atactacttg atactttgat gtctttgatg aaattactta gtgaaacgga
    14701 gggatgcagg tcagacaaaa ggtggatatg atccggcatg ccatttaatt gatgaagaac
    14761 acaattgttt tttttaacca caccccagat gtatttgaag agatccagtt catggtccgg
    14821 gtttatagcg ggttctctga attttgttcc aaaaacaatg tgataataga cttgtctgta
    14881 cgaactcata aacaattttt taggaataag gcccggattg agttccggtt ttcaaatcta
    14941 taagggaaat atttgagaat tctattcgtc tccatttgaa ggaataaatg ttgaaaactt
    15001 ttcgaatatg gcaattggaa caccggtttc accggttgtt agaattattc aaccccgttg
    15061 gggttggggg ctgtgggtgc cttttaccgc cagtttcact ggcggttatc gttgttcaac
    15121 ccctatcggg gttggttgat tgtcaaacta ttcagatggg atttagttgt tatgtcagga
    15181 tcactggctg gcagataaat ttatttagtt ccggattctt tttcacgcta catcggagat
    15241 atcggggagg ttgatagtta caattttgta gatgcactgc ctgaaataca aattttctca
    15301 accccaacgg ggttgaagaa tgatagcccc tggtgaaacc aggggtataa taattataga
    15361 gccccctaca accccgaatg gggttgaatt aaatcagcaa cattgacgaa acatcaaacc
    15421 agtttttccg gtttcaacgg caaatgggtc agtcttttac cggtggcatt atataccgca
    15481 ttggccaatg ccgaagcgaa accgattaac gcaatttcac ccactccttt cgaacccatc
    15541 gggttgatca gcggatcagc cagatccgga aaccaggtat gaataaaggg aacatcccgg
    15601 tgacggggta cctgataaga gccgagtgta tcagtcaggt atttgccggt agtcttatca
    15661 atcaatgctt cttcagtcag tgccatacca atgcccccta cggcaccgcc aatcatctgg
    15721 ctgcgggcag ttttcggact cacaattttt ccgccatcgc cggtgcagac tactttcagg
    15781 aattcaatcg ctcctgtggc cggatccaca gccactttaa taaaatgcgc agagaacgaa
    15841 ttcatcgcgt atttgctgcg tactgccgga tcaggaccac tttctttcag cacttcaatc
    15901 tgtggtttgc cactggcttt gagcagttcg gtcagactga ttttcttatt tgtctctttt
    15961 tttgaacgga tggactcttt atcaaattca agatccgctt ccgacaatcc attgaataat
    16021 ggcgattgtt ttaaggcaat ctcggtaaac tgcgctttca atgtatcaca taccaaactg
    16081 accgcggttc ccattgtgga agtggtggtg gaacctccct gcgtagggcc gggcggaaat
    16141 tcagtatcac ccagtagaaa actgatttta gatgatggca tattcattac ctgtgccgct
    16201 atactgacca tagccgttgc tgtacccggt cccatatcgc ttactgcact ataaagcagg
    16261 agctttcctt cgctggtgaa aacggctttt acggtggcat tacccctgga agcgccaaac
    16321 gcaccaccgc ccattccgta tccgatcaat ttaccatttt cggtcaggga acggggcgta
    16381 gccggacggt tggaccagcc gatcatttca cgccccttat cataacattc cctaaggaac
    16441 ttactggtcc agggtagttt actctgagga tgaagtaccg cgtaattttt tatccggagt
    16501 tccaccgggt ccatatttag tttataagct aattcgtcca ggatacattc gagcgcaaaa
    16561 gcaccggtgg cttcaccggg accccgcatc caggtggggg tgtttacgtc cagcgggaga
    16621 attttataac tggtgttgac attcggacat tcatacagat aatgcgaagc gttcacgatc
    16681 ccttcacggt aatcttcata gcgggaagta ttgctgatgg catgatgggt gatgccggtg
    16741 agtttgcctt ccttcgtggc accaaccgct attttctgcc aggccatggg acgataaccg
    16801 accatggtaa acatctgccg gcgggtcagt tgtaccctga ccggacgttt caccattttc
    16861 gcagcgatgc aggcggccgg tacatgcggc cagctgcgca acgaagaacc aaaagccccg
    16921 ccaaggaact ttgtgatcac gcgtacattt ttttcttcga taccaaaaag ccgggccagt
    16981 tccgattgtg tgcctttcgg accttgtgat ttatcataaa ccagcagttt gtcctcgcta
    17041 tcccattggg caatggtggc atgcagctcg atggggttat gcgtttcaac aggcatatag
    17101 tattcgttct ccacaaatac ttccgcctgc ttataggcat cggcctgtcc tctcttatat
    17161 tcacctgagg gcttcagttt gctttctgtt ttacgcaggc tgttaaaatc agtctctgcc
    17221 acttcgttga tatattctgc ttttacgagg gaagcggctt tcactgcttt ctcccaggtt
    17281 gtagctacca ccagtgcaat cggctggcca taatgataca cgaggttctg gtggaagggt
    17341 ttcaatcccc tccattcaaa gcccttcttt ttgggatctt tagcccaggg attatagccg
    17401 ggcagggcag gacagttcag gtgcgtgatg atctccagca cacccggagc atctttcgct
    17461 tcgttcagct tgaagttttt aatagcgcca cgggcaatgg tactggtgac aaatacacca
    17521 tagaccatat tttcgggatg atgatcggct gtgtatttag cccgaccggt tactttgtcg
    17581 atcccatcca cgcggtcgtc ttcgtccgct aagaaattcg ttatatattc ctgatccata
    17641 ctggtaagtt gaaaagtgca ggtgattaaa ttttttgtcc gctgttcttc gcctcgcgta
    17701 tcgcggccac gatattagga taagccccgc aacggcagat attatcgctc atatattccc
    17761 ggatatcggt ttccgtgttc gcctgtcctt cccggataca ggccacggcc gacatgattt
    17821 gtccgggtgt acaataaccg cactggaatg catcatgttt aataaaggct tcctgtacgg
    17881 gatgcagttt gtctccatcg gccaatcctt caatagtggt gattttttta ccattgttat
    17941 ccaccgcttc cgtgagacag gagttgaccc gtttcccatc cacatgtaca atgcaggcac
    18001 cacaatggcc ctgatcgcag ccttttttag tgccggttaa tccaagttgt tcgcggagca
    18061 tatccagcag actaacaccc ggatccacga ggacctggac cgtcttacca tttacttcca
    18121 tttgcagcgg tgtcttttca aaaaaagaag caatcgcttc atggttttca ccggctttta
    18181 ccacggcaat tggcgtgagg ctgatagcgg taagggcagt ggattttttg ataaagtcac
    18241 gccggctttt gccgggacag gaatgaacat gttgttccat agaaaaaata tatgcttaaa
    18301 gttaggttat aaatgcgcac atgaaaaaat aatcaggagt cgggaaaagg agctggccgg
    18361 actttattcc cttttcggca agccgggctg attgcctacc tttgtccctg ttttttcacc
    18421 ggctttgaca atcaatatga accgaattta cctcgataac gcggctacca cgcccatgga
    18481 cccccaggtg ctggaggcca tgttgcctta catgacttca catttcggaa atccttcatc
    18541 tgtatattct tacggcaggg aaacccgcct ggcgattgag aatgcccgga agactgtagc
    18601 ccgtattctg caggcgaatc ccggggagat cttttttacc agtgggggca ccgaaagcga
    18661 caatatggcg attgcatcag cgatccatga cctgggatgc cggcatatca ttacttcagc
    18721 tatcgagcat catgcggtac tgcatacggt ggaacattat gatcaccggg atgaagtgac
    18781 cattagtttt gtgaagttgt tatcggatgg acatatcgat ctggaagacc tggaagctca
    18841 gctcgctgcc cggccggagc gatgcctggt gagcctgatg catgccaata atgagatcgg
    18901 aaacctgctc gacatttacg caaccggtga gatttgtaaa aagtataatg ccattttcca
    18961 ttccgataca gtgcaaacca tcggacatta taaaattgat ttacggaaga atccggttca
    19021 ttttgccacc gcggccagtc ataagtttca tgggcccaaa ggagtgggga tcatttatat
    19081 taatgataat atcaaaatca atcccatcgt ttttggcggg ggacaggagc gtaatatgcg
    19141 ggcaggtaca gaaaacctgt atggaattgt cgggtttgcc aaagcgcttg aactggccga
    19201 cgccagccat gaaaaggaca gtacttatat tcaatcgctc aaacaatata tgattgagca
    19261 actggaacag catatacctg atgtggcatt caatggtgat tataaagggc agtgtttata
    19321 tacggtgctc aatgtgtctt tccccaagac cgagaaatca gagatgattc tcttcagcct
    19381 ggatatgcag ggagtctgtg cttccggcgg tagtgcctgc agcagcgggg ctgatatcgg
    19441 aagtcatgtg atccgggcca ttaacaacaa tcccaaccgg gtaccggtgc gtttttcgtt
    19501 cagcaagtat aatacgaagg aagaggttga tgtagttgta gagaagttga agcagctgat
    19561 ttgagagttt gacgattgta gtttgttgtt tggcgatcct tctgagaaaa tatttcttaa
    19621 atcagtgcga atccgtgtat atctcccgca taatgcagga caggcgtgtg acctgccttt
    19681 ttgtatttct ttcgtgcaca tttgtgtcat ttagtggcca ccttatttgc cgtaggcaaa
    19741 tcaatccgtg tttatctgtg ttatccgtgg cttgcctttt tgtatttctt tccccgcatg
    19801 aggcgggaca ggcgtgccta tttgtgtgaa ttcgtggcca aataatcccc gcaggggatt
    19861 ttaacaaata ataatttggc cgtctacgaa aaattcgtag gtttacgaaa atttcgtaga
    19921 aaatggaaaa actcacccat gccgaggaag aaaccatgca ggccgtctgg cgtaccgggg
    19981 aaggcaatgt aaaagccttt atggaatacc tggacaatga tatcccttat actaccgtgg
    20041 catccaccat caagaacctg gagaagaaag gctacctgac cagtcgcttg ttggggaatg
    20101 cctatctcta tcatccggct gtatcggaag aagagtacaa ggaaaaattt atgggtaaag
    20161 tggtgaagga gtatttcgac aattcctaca aggaactggt caacttcttc gtggagcaga
    20221 aaaaactttc tgcccgggaa ctgaaagaga tcattcaact gattgaacag gggaagtagt
    20281 gagtggtgag tggtgagtgg gtgtactaaa gatgtaaatg tctcaaagtc taaactattt
    20341 ccaatgcctt ttatttttag ttatttattg aaattatcaa tcagcttaag cgtggtattc
    20401 ctgttttacc atttcgtatt gcgcaggctg actttttata cctggaaccg ctggtacctg
    20461 attggatatt ccgttttgtg ttttttcctg ccttttgtcg acatttcatt cgccttggag
    20521 caatcggata ttaaaaacag tccggttgta aaatgggtgc cggttttggg tcagcgtatc
    20581 ggactagaca caggtgcgga tagcggtccg gtgagtggct gggaatatgc tttgctggta
    20641 ttaatggcgg gggttggggt gatgctggtc cggctgggta ttcagctgat ttccatttac
    20701 cggatgagaa aacgttcgtc catgatttcg caggacggta tccggatcta ccaggtagat
    20761 gcaccgatca tacccttttc gttcggtgat tccattttta tcaatcaaca tcagcatagc
    20821 ctggaagaac tggaagaaat catccggcat gaatttgtgc atgtcaaaca aaaacacagt
    20881 gtggatatta tctggggtga gctgctttgc ctgctgaact ggtacaatcc ttttgcctgg
    20941 ttattaaaga aatcgatccg gcaaaacctt gaatttattg cggaccgtca tgtactggaa
    21001 caggggatgg accggaagca ataccagtac ctcttattga aagtaatcgg gaacaatcat
    21061 tttagtatag ccaatcaatt taatttttcg tcacttaaaa aaagaattgc catgatgaac
    21121 aaacttcgct ctgccaaatt ccatttggtg aaatttatgt ttgttcttcc gctggtttcg
    21181 gtcatgctgc tcgcattccg gaatgaatgg aagaaacagg aaccccgtac tgaacaatta
    21241 atgacagcag taattgcggg taatgatacc atacccgata aattaccacc accaccacca
    21301 ccgccaccac ctccaccaaa ggggcaaggg acaataaaag attttatcga cagaaatcaa
    21361 ggggtgaaga atctgggttg ggtatatcac catgataatt ctgtagcgat gatacatgtc
    21421 atgaaaaaag atggagcgac tgaaatctat catactgata atgcagaaga aatgaatgca
    21481 ggcgctaaaa aatatggcaa gtttccggtt cctccgggtg caatggcgcc tccgccacct
    21541 ccgggtcagg ataagcatgt ttgggtacag ggatatccga ttgcacctgt cgatcccaaa
    21601 gatgtggtag tagtacaggg atacccgatt gcaccccctg cccccgcaag cggagttgtg
    21661 gtggaaggct atccgatagc accggtccca ccccatggag aaatcgtggt gactggttat
    21721 gcaagaccgc ccgcacctgt taagttgccg gatcatgtta aatccatttc cattaaaaac
    21781 ggggataagg ccgtggtagt gcttaaaaat ggcaagacag agctatatga cctgaacaac
    21841 cctgagcaaa agaagaaatt tgatgctcaa tacccggctc ctccgccacc accaccgcca
    21901 cctgcaaaag gggtacgggt ggaaggacgt cctttgaatg gatcagtagg tgcaacggtt
    21961 gtggaaggca aacctctgcc tgataacctg atctatgtga ttgacggaat tattactaca
    22021 aaatttgaaa tggaaaagat cagcccggat aagattgctt cgatagatat cctgaaagga
    22081 gaatctgcag tggccctcta tggcgaaaat gcgaaggaag gggtggtgca gataaaactt
    22141 aagtatgagg cacagccttt gtacgtgatc aatggagaaa tctcatcccg ggaaacaatg
    22201 gacaaattaa aacctgagca aattgaatca gtcgatgtgc tgaaagggga gaaagccaca
    22261 gcagcgtatg gcgataaagg taaaaatggt gtgataatga tcaaaacaaa gaaataagta
    22321 tagccctgaa accaacaggc gccattccta agggagtggc gtttttttta caggtaatac
    22381 cagggattgc ggcgggcggc cattacgtat ttacggacat tgcggaaaaa ggcaagcacc
    22441 atataaatga taatcggaga gcccatggtc agcacggagg catacataaa gtactgccgg
    22501 atccggctgg tggcgatacc caggcggttg ccaatggcag aacaaacacc aaaggcgttc
    22561 cactcgacaa agtctttgaa tctgttcata aggctaaaat agaaaattta aacaaatgcc
    22621 ccaaacctga gttataatag atgacctgtt tttcgtactt ttgcggcaca ttttcaatta
    22681 gccaattagc cgatttgcca attagccaat ttgaccttcc ttcgataact tccctaatgt
    22741 cagcaaattg tactaaaacg ccggttgtgg atgtttaatt accgcatttt ctaattatca
    22801 aattgtataa ttaccatggt attcgatctt gatcttatca aaaagactta cactgaaatg
    22861 cccgccaaag tggatgccgc ccgtaaagca ctgggacgtc ccctgaccct ggctgagaaa
    22921 attttgtatg ctcacctttt tga
//
LOCUS       DAOPMW010000016        22368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2158_length_22368_cov_9.666607, whole genome shotgun sequence.
ACCESSION   DAOPMW010000016 DAOPMW010000000
VERSION     DAOPMW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 22368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22368
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_22368_cov_9.666607"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1265)
                     /locus_tag="PLU11_01740"
     CDS             complement(99..1265)
                     /locus_tag="PLU11_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57855.1"
                     /translation="MNRVFVLLLSILFFSSCDSVTQKSGGKAVNPDGTPMQEGKDYMI
                     LKRFRVVDNQGFSQPVEVSSFLLPANWTVKSEVNWNATSKCLPEMLQASVQASSPDAA
                     YELLVYPATQFDWSDDPIYLDAMQKGFVMHSCTAAQPQDAAGYISGTLAPFMRANVKS
                     ASIIESLQQQMDAGAMQMTQAARQGGNTAYNHKGSAAEGVLQFEDGKEGLAFCTLMQT
                     LVSVPGAQGGMSTNTQCYVSMRVVIKYKPGNEPMARKIMSTFFSSARINPQWASGVQS
                     YFAAVTRNAQDEGWKQIQISHQAQQEIGDNIIRSWESKNASEGSRASDTESFGQYLRG
                     VDNWTDEGGNKVELTSGYSNAWSRGDGSYMLSNNPAFDPNTIAGETQSWSRLKQ"
     gene            complement(1321..2493)
                     /locus_tag="PLU11_01745"
     CDS             complement(1321..2493)
                     /locus_tag="PLU11_01745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN57856.1"
                     /translation="MKSLQTFLLLFLSGILFSCSKSGGGSDPVPATQADIAAVDSKVN
                     TFMTTYNVPGASLAVSKNGKLVYVKGYGKANTSTNELVTPAHRFRLASVSKTFTGVAI
                     LKLVQDGRLSLDAKVFGTGGVLANDFGTSPYNSNLLNITVRHLLQNVSGSWGASTGGD
                     VIDQNPSYTYKQLLDWIINTRPNPNVPGTVYDYSNVNFCIAGRIIEKISGKSYVNFIK
                     EDIMAPIGATQTDISGKTAGEKKANEVTYYGQGQDANFVYNIAFPRRDADGGLLSTAS
                     DLLRLVNAIDGFSTRTDLLTPASITALTTPSAVFSSYACGIGIWSAENLWYNYGSLPG
                     TRTGFFRHNNGMCVALLLNSRVDPVAGENPFVFAMQDLMLDLVKNSSYTWQNIDQF"
     gene            2625..4580
                     /locus_tag="PLU11_01750"
     CDS             2625..4580
                     /locus_tag="PLU11_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN57857.1"
                     /translation="MMKRIGYLLLPALMAFCSANGQFPGLDRVREAIYGASNDQERLS
                     RLQAASAFKNSMHGDTIYHYAKWAKELALKLGDKKALARAEYSLISGDIASGKTDSVM
                     PRIDRNKIFIGIKETDPDLFYKIQLLKANVLNRTSRLNEAQELQLQLLQSAEKEKNTM
                     ARLFLLNYVGATYLNTTGQRETARKTWESGLDIIRQTNDPAYSEIETYLLSNLSLYFF
                     GNYYAAPTPERFDTCLRFLNRTIDLCRKNESMGVLASTLSYRGNLYGRNKMYKEAEAD
                     FTATVEIRNKIGDPLYITEDLKNLSFFYFERNELDKCLAVINEGLLTCERFHIRETVL
                     DLLKMKAGVYQKKGDKEQYITVLEKLLLTGDTIFQVRTAEKIAKLQTEFDVQKKEALI
                     AKQQLDLLKRNFLLYAAGIATFLLALYFVFRFRKYKRKQQLLMEEKKQQHALQVKEAE
                     EKERKRIAAELHDNLGVQANAILYNSSQLSAGTGNTVQIANDLQETAKEMLLNLRETL
                     WAMKNSDVNAVQLWLRVINFMQQMGRHYTDIQFKVSGTAPKDLIIPANRALHIVLVIQ
                     ETVNNSVKHARATTIIAQSILQETDWTILLQDDGTGFDLEKAQTKEDRYGLKNIQERA
                     LAGEFSCRISAEPGKGTLTTLQISHSR"
     gene            4632..5297
                     /locus_tag="PLU11_01755"
     CDS             4632..5297
                     /locus_tag="PLU11_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57858.1"
                     /translation="MIRVAIADDKIYNRKVIVDKLARTALFSLVFQAADGNEFLQKMR
                     ELKQEELPHIVLMDLEMPEVDGVSAIGTASALYPSVKFVVLTIFDDDDKIFRAIRAGA
                     CGYLLKEESGEVITDMLMNLWENGAGPISPSIAYKILQMVQQPVAVTDEKNKAENIFQ
                     LSDRELEILQLLCEGLEYKEIGNRIFISANTVKKHCISIYQKLHVNSKAQALRIAYTK
                     GLV"
     gene            complement(5316..6662)
                     /locus_tag="PLU11_01760"
     CDS             complement(5316..6662)
                     /locus_tag="PLU11_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN57859.1"
                     /translation="MSAYTIYMVEDDPWYGEILDYHLSLNPDYKVTRFESGKDCLAKM
                     HLKPDLVTIDFSLPDITGDDLFKRIREISPQVPVIVISSQEEVAVAVNLLKMGVNDYL
                     VKDEATKDLLWNSIIRIRETQSLKKEVEQLREELGQKFSFQKTMIGQSESLKKVFTLI
                     GKAVKTNINILINGETGTGKEVVAKAIHYNSDRKKKNFVAVNMAAIPRELIESELFGH
                     EKGAFTGAVGRKPGKFEEANGGTIFLDEIAEMDMSLQSKLLRVLQEREVVRVGGNEKI
                     KLDIRLVTATHKNLAQEVQRGNFREDLYFRIMGLPIVLPPLRERENDILLLSKHFADE
                     FAKENNLGTIQFSKEARDKLMSHPFPGNVRELKAVIDLAVVMCEDNEIREEDITFSQG
                     KRDDLFITENKTLRQYTCDIVKQYLKKHNNDVVAAANALDIGKSTIYKMMQAGELD"
     gene            7135..8481
                     /locus_tag="PLU11_01765"
     CDS             7135..8481
                     /locus_tag="PLU11_01765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPN57860.1"
                     /translation="MKIVIVGSGYVGLVTGACFSEVGINVTCVDIDKKKIENLNNGII
                     PIYEPGLEDMINRNVKKQRLTFTTDIKEAIQDCEVVFIAVGTPPDEDGSADLKYVLSV
                     ARDCGRNMNDYLLIVTKSTVPVGTSEKVRKAAQEELDKRKVSIDFDVASNPEFLKEGA
                     AIDDFLKPDRIVIGLESTRAEELMKSLYKPFTLNGHPLIFMDITSAEMTKYAANSMLA
                     AKISFMNDIANLCEIVGADINLVRKGIGSDSRIGNKFIYPGIGYGGSCFPKDVQALIR
                     TAGEYNYELRVLKAVEAVNKDQKMVIYNKIMNYFDGDIQGKTIAIWGLSFKPQTDDMR
                     EAPSLVIINMLLEAGAKVKAYDPVAMKEARHHFGETISYVEDPYEALIDADCLAILTE
                     WPEFRVPNFKIIKKLLNNAVIFDGRNIYDKAEMNRNGFNYFCIGVQTTTKQKTELV"
     gene            8533..>8830
                     /locus_tag="PLU11_01770"
     CDS             8533..>8830
                     /locus_tag="PLU11_01770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN57861.1"
                     /translation="MNRKKILVTGGAGFLGSHLCERLLHEGNEVYCMDNFFTGQKQNI
                     VHLLDNPYFELIRHDVTAPFFIEADEIYNLACPASPIHYQYNPIKTMKTSVMGAI"
     assembly_gap    8831..8930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8931..9626
                     /locus_tag="PLU11_01775"
     CDS             <8931..9626
                     /locus_tag="PLU11_01775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN57862.1"
                     /translation="HYQYNPIKTMKTSVMGAINMLGLAKRIKAKILQASTSEVYGDPK
                     IHPQPEAYWGNVNCIGERSCYDEGKRAAETLFMDYHKQNGVNVKIIRIFNTYGPRMHP
                     NDGRVVSNFIVQALQNKDITIYGEGNQTRSFQYVDDLIEGMIRLMNTPKGFTGPVNIG
                     NPNEFTIRELAENVIRLTGSTSKIVKMPLPADDPMMRKPDISLANKELNWNPNTQLEE
                     GLLKTIAFFKTVI"
     gene            9881..10225
                     /locus_tag="PLU11_01780"
     CDS             9881..10225
                     /locus_tag="PLU11_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57863.1"
                     /translation="MPEQLYNLNLVKKLAHNNMDIINKLMHVFIEQAPTSVKDIKSAY
                     YSKKYAVVTALAHKIKPTYGYFAVNETEKDLEMIERLSTLESPSAEIEEMINKVEVAT
                     MKLVAEMKADLN"
     gene            10380..10766
                     /locus_tag="PLU11_01785"
     CDS             10380..10766
                     /locus_tag="PLU11_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN57864.1"
                     /translation="MKKTILVLDDESIIGLLLENFLSAQYEVKHINNGQQALEWLEIN
                     IPDLIICDIEMPEMNGFDFLTQVRQRGFTRHTPVVMLSAKSESKERIKCYKLGAQDYL
                     TKPFNPEELEEIVKKNLFPIHFSIAW"
     gene            10784..11980
                     /locus_tag="PLU11_01790"
     CDS             10784..11980
                     /locus_tag="PLU11_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPN57865.1"
                     /translation="MKTSLSVLYVGNNPAYFQPLENKGEIALKTVSSSVRGIKYLSEG
                     NKTDVIFSDFCLSGSNGLFFFDWIRTQPGFDKVPFILLSKEFRSEIYKTAYQKKVDDY
                     YSLAQPVPEDISTRLRFLVNFKKKKGVYVDEKTSAKFSMPLSKRLFDIAVSATALLLL
                     SPILLLVILAIRLESKGKVYYISRRVGRKPFDFYKLRSMRTGADSQLKALAKIRNQYA
                     DTSANAEVDFTLPCPRCSNMPDGQSCSPILYIKDHAICDFWHNEQQKNVAAKNSVFVK
                     IKDDPRVTRVGKFIRNTSIDELPQLINVLKGDMSIVGNRPLPVYEAEQLTRDDLSKRF
                     LAPAGITGLWQVELRGKGGVMSEDERKRLDNEYADHFKDGKYSFWYDVKLMLRTIPAM
                     FQKDSV"
     gene            12000..12728
                     /locus_tag="PLU11_01795"
     CDS             12000..12728
                     /locus_tag="PLU11_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN57866.1"
                     /translation="MKLTAIYIITLLLILSARPGQAQSADSTAMTPSDSVITFPPLND
                     LIDSAVKYHPGIRYRQYEVKVKESNLQTQQHNWTRNMGVQADSRYGTFDNFSANDNGQ
                     STTLLNTTNKQFNYGFGVYMKLPLYDLLNRKNQLKQAKNEVEMARSLGEVQETEIRQM
                     VIKQYQDALLRQRLIRIKAQNLGNARVNMEMVEKEFRNGIIPISEYARISDIVYRAES
                     DYELAKTEFITAKLLLEEMVGFKF"
     gene            12744..15182
                     /locus_tag="PLU11_01800"
     CDS             12744..15182
                     /locus_tag="PLU11_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57867.1"
                     /translation="MKIIELIRLIRKHLVLLITVSVLAAVLVTMLTRNPKLTFASKTT
                     LYTGLATGSTVEMDKTYNYALTNTSFDNLINIIKSRETQQNVAIRLLSQHLLLPGPDP
                     KYISKQSWEELKRITPEYIYNYVVKSKPTNTITTDTIPVKTKNKGDSLRTRSANTHSA
                     DSSQILYKSGLFPASVSRLDFEKTVYNLTQLMHSSDTNFVYKLLNYTDPHYSIAAISG
                     VKAERIASSDLVRLSYETDDPGICQQTLAIYSEVSINNFKNIKENRSDAVVKYFESQL
                     KQADDKLKEAEDDLLAFNKSNNIINYYEQSKAVAVVKEDMEVDYNNKKAQLAGIEAAT
                     RRLEDKLNIQQVVQLKSGVVMDKKKRLSEVNYLIAEAEADNKADGPLKALRNEANVLK
                     EEIRKNVGDLYAYQNTTDGLPVSKVLNEWINSVVEADNLRASIRVMDQRNRDFQQQYS
                     IYAPAGAHIKRIEREIAVSEQGYLEILHGLNLAKLKLQDNELSSNLKAVDLPFYPLTP
                     TPTKRKVLIIAGALVSAMLVLGLILVLEYFDETLKNLKRAAKTLQLPALGIIPKVYLH
                     PETSNFPYIQNRLLEFSIQNMEQFFGLNNGSKITKTILFVSTQENEGKTTLAGNLALK
                     LTRQGKKVIVLNYNHQPKNTSKHSKFPIFSRLLGYSDPRIDYNSPFLAAPENYLPKEN
                     CFTYTVNDDFYNSRSYTEILEHNGQQAAFVPDYVLIELPALLSKNYPTELMSMSDLVV
                     LVCRANRIWTESDQLVINSIQPLTGNKLHFIINGSQLKEVEAVIGDLPKKRSILRKKL
                     KNLFRFQFFSSNRI"
     gene            15192..16565
                     /locus_tag="PLU11_01805"
     CDS             15192..16565
                     /locus_tag="PLU11_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57868.1"
                     /translation="MKKTLNKIIREDNFLSLAGNLVIAAAGLAGFALLARSLQLNQFG
                     EWMLFISSGSFIEMFRFGITNTGLVRYLSGADEHTRIKLIGSNSVISLISSLVIIALL
                     VICNLLFHTSLEKAGYGLFFTWYPLLALVNLPWNNALVVLQADRKYGKMLLIKSMNSL
                     PFLMVVLVNYFTGYFTLTQLAIALLIINALTSLISLAAGWDGLRYIFKATRASTNTLL
                     HFGKYTTFTLVGTNLLRSADSIIISLSPMGVNAVALYSIPLKLTELQQIPLRSFAATA
                     FPKMSKASIHGKLDELKKLFYTYTGAITYLFVGVSLITFVFADFFVLVLAGKSFLQNN
                     PDAGQAISIVRIFSLYGLLLPLDRMTGIGLDSINKPGVNALKVLIMVLVNIAGDLIAV
                     FVFQSLLLVAISSILFTAMGIWLGMYFLNKELHLRYRDIFTEGAWFYKNILNKVSGNR
                     LAVNLNR"
     gene            16578..18056
                     /locus_tag="PLU11_01810"
     CDS             16578..18056
                     /locus_tag="PLU11_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN57869.1"
                     /translation="MTTGQHINPFEYNTGSSRLSSPRAVLLVIMIISGLAFLSAKLGM
                     MSFAVSVGLPLFLVYGYFLFTWPVVGLYTAIAMSFVLIGAGRYVKDVPVGLGLDAVLI
                     LTFIALFINKFKEKVDWSPVNKDITYLSLIWFGYSMFQLVNPESQSVAAWFSGRGVAL
                     YMFLMVPLTLLLINTNRKLDILLYVWGALSILATLKGIMQLKIGLDAAEQGWLDDGNA
                     KTHVLFGKLRVFSFMSDAGQFGGNQAYSAVVSFIFSMAQTDRKKKWFFLIVSLLAFYG
                     MIISGTRGAISVPLAGFMLFFILRKNIRVLSIGVAILAVVFVFFKYTTIGQGNDQIRR
                     MRTAFDPNDASLQVRLDNQKLLKRYLASRPFGGGIGHGGVKAQKYLPNAFLSQIATDS
                     WYVLVWVEMGIIGLLLHLFILFYTLIRGTWLVMFRLKDPKLKLKISALIAGLAGVMVA
                     SYGNAVLGAMPTGMLIYTSMAIILNAKALDKSTGEPVPVLIK"
     gene            18083..19297
                     /locus_tag="PLU11_01815"
     CDS             18083..19297
                     /locus_tag="PLU11_01815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN57870.1"
                     /translation="MTSTMTEKMKGKDFIITGLQSWDIPIGSNAIDIAKEIAKNNRVL
                     YVNSPLDLMTMYRNKKTPENEHRLDVVKKFKKPIRQISENLWVLDFPFAVWSVNGFPD
                     GPVFDLFNRRNNKRIFNYVKKTAAELGFENPVHFIDNDIYRSFYAKEILKPRLSVYYR
                     RDNLQPFAYWKKHVARLEPALIGKSDLVVCNSPELADFSRQFNQKSFDVGQGVDLSAY
                     DPDKKFPIPAELATIPGPIIGYIGDINSLRLDPDLIYELAKARPQYSFVMTGREDKVF
                     AAHALHTLKNVHFTGSIPKDRVPEFMAAFQVCLNPQLINEITIGNYPRKVDEYLAMGK
                     PVVATTTNTMQLFKEHVYLCKNAGQYMVAVDKALSEDNDRLRQQRIAFARSHSWENNV
                     QNIYNQIQQSLN"
     gene            19303..20259
                     /locus_tag="PLU11_01820"
     CDS             19303..20259
                     /locus_tag="PLU11_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPN57871.1"
                     /translation="MNYRNIADLNKVILKKLSIIPRDIDLIVGVPRSGMMPANLLALY
                     LNKPYTDLHSFINGHIYKAGARAQFFDANSGSKKILVVDDSVASGRALNEVKELLKDH
                     STRFDIKYCAVYMSPGKEKTVDYFFETVALPRYFQWNIMNHSSLEKACFDIDGVLCED
                     PKPEQNDDGEKYTDFILNAPPLYIPGSKIGTIVTSRLEKYRKETEIWLQKYNIKYNEL
                     IMLDLPDMKARQEANNHGGHKANAYLSKPYTLFVESELGQAREINRLTKKPVFCTETF
                     EMIFESESVLYNLKSGKYLPFLRKYALKARTKIRKMKNARHA"
     gene            20302..21489
                     /locus_tag="PLU11_01825"
     CDS             20302..21489
                     /locus_tag="PLU11_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN57872.1"
                     /translation="MLVYNIIQVILLSLLGSATLYIFIFAVAGVFYKEKKSAKADRHR
                     KFAVLIPGYKEDEVIVEVAKSALLQEYPMGLFDVVVIADSFQPETLAELRTLPVKLIE
                     VSFDKSTKSKALNKGMATLTSHYDIAVILDADNIMAPDYLLKMNAAFAQGAFAVQGHR
                     TAKNQNNSWAVLDAASEEINNHIFRKGHRVLGLSSAIIGSGMAFYYDFFKTMMADVKA
                     VGGFDKEIELKMLKAKHRISYQDDAMIYDEKVQKADVFGNQRRRWLSAQIHYFQKDII
                     PAIGHLFTKGNLDYFDKAIQFIQPPRVLLIGALLLLSTLFIGANYLLNNGISYSTAWL
                     AITAACMLALLLSVPRSFYNWKTGRALISLPSGMLVMLMSLLKTKGANKTFIHTKHTS
                     SSR"
     gene            21515..>22368
                     /locus_tag="PLU11_01830"
     CDS             21515..>22368
                     /locus_tag="PLU11_01830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPN57873.1"
                     /translation="MKKNRLEKLEALRGFAALYVVLFHVLPQQILLFGVNIGLLFRFG
                     PEAVIVFFVLSGFVIKYTFERSADKSFKYYFIRRFIRLYIPLFFIYVLGYLLKCYNAG
                     ELANPEWGSLLGNLFMLQDVISQKPNVISAAYMGNGVLWSLSYEWWFYMLYYVLATRI
                     PAEKLNKWVNIIVITAAASYLIYPFIVNRLIMYFAIWWIGVRFAEIYLQGGAFSFKKV
                     MPYGAVLLAITCILGLNLYLNFSYTKVYSYPLVAYPFIEVRHFVFAIIVMFGAIAWYN
                     LKWVGFNAA"
BASE COUNT         6375 a         5351 c         4791 g         5751 t
ORIGIN      
        1 ttatcgtacg ttcaggcaaa aatcagaagg aggtcaccac attatgacaa cctccttttt
       61 tacatagaag aaaggacgga agaagccgga taaaatggct actgcttcaa ccggctccag
      121 ctttgcgttt ctcccgctat agtattcgga tcaaaagccg gattattact cagcatataa
      181 ctgccatctc cccggctcca ggcattggaa tagccactcg tgagttccac tttatttcct
      241 ccctcatccg tccagttgtc cactccacgc aggtattgac cgaaactttc cgtatcggaa
      301 gcgcggctgc cttcactggc attctttgat tcccagctac ggataatatt atcaccaatc
      361 tcctgctgag cctggtgact gatctgtatc tgtttccaac cctcgtcctg tgcattccgg
      421 gtaaccgccg caaaataaga ttgcacgcca cttgcccatt gcggattgat ccgggcactg
      481 ctgaaaaatg tactcatgat tttccgggcc atcggctcat tacccggttt gtattttatt
      541 accacccgca tactcacata acattgggta ttggtagaca tcccgccttg tgccccgggt
      601 accgaaacca gtgtttgcat cagcgtacaa aaagcaagac cttccttccc atcttcaaac
      661 tgtaatacgc cttccgccgc actgccctta tgattatacg ccgtattgcc tccctgcctt
      721 gcggcctgcg tcatctgcat cgcccctgca tccatttgct gttgcaggct ttcaataata
      781 gacgcggatt ttacatttgc ccgcataaaa ggagccagtg ttccactgat atatcccgcg
      841 gcatcctgcg gttgtgccgc tgtacaagag tgcatcacaa accccttctg catggcatca
      901 aggtagatgg gatcatcact ccagtcaaac tgggtggcgg gataaaccaa taattcatac
      961 gctgcatccg gtgaagaagc ctgcaccgat gcctgcagca tttcaggaag acatttactg
     1021 gtggcattcc aattcacttc gcttttcacg gtccagttag cgggtaataa aaaggaactg
     1081 acttcgactg gctgactgaa tccctggttg tccactaccc gaaaccgctt caggatcata
     1141 taatctttgc cctcctgcat cggggttcca tcagggttta cggcctttcc gccggatttt
     1201 tgcgttacgg aatcacagga cgaaaaaaac aggatagaaa gcaacaggac gaaaactcgg
     1261 ttcatattta taaaattcag tgtgttcgtt atagttatat tattctccgg aatcggtttt
     1321 ctaaaactga tcaatattct gccaggtata actgctgttt tttaccaggt ccagcatcag
     1381 gtcctgcatc gcgaaaacaa atggattctc accagccacc ggatccaccc ttgaattgag
     1441 caggagggcc acacacatcc cgttgttgtg ccggaaaaat ccggtacgtg tacctggcag
     1501 actgccataa ttgtaccata gattttccgc actccagata ccaataccac aggcatagga
     1561 ggagaacacc gctgagggag tggtgagagc ggtaatactg gccggggtca gcagatcagt
     1621 ccgggtactg aatccatcaa tggcattgac cagtcgtaaa agatccgaag cggtggagag
     1681 aagccccccg tctgcatccc ttctcggaaa agcaatatta tacacaaaat tcgcatcctg
     1741 tccctggcca taataggtaa cttcattggc cttcttctct cccgctgttt taccgctgat
     1801 atctgtttgt gtagccccga tcggtgccat aatatcttcc ttgataaaat tgacatagga
     1861 tttaccggaa attttctcaa tgattcttcc ggcaatacag aaattgacat tggaataatc
     1921 atacacagtt cctggtacat tgggattagg ccgggtattg ataatccaat ccaggagttg
     1981 cttataggta taggacggat tctgatcgat cacatccccg ccggtagaag ccccccagga
     2041 gccggataca ttctgcagca ggtggcgtac cgtaatgttg agcaggttac tgttatacgg
     2101 tgaagtacca aaatcattgg ccagcacccc gcccgttcca aataccttgg catccaggga
     2161 aagccgtccg tcctgcacca gtttcaggat cgccacaccg gtaaatgttt tggatacact
     2221 cgccagcctg aacctgtgag caggagtcac cagttcattg gtactggtat tggctttccc
     2281 atatcctttg acatacacca gtttcccatt cttgctgaca gccagggaag ctccgggtac
     2341 attgtaggtt gtcataaaag tgttcacttt actatcaacc gcagcgatat ctgcctgggt
     2401 agccggcacg ggatccgatc caccaccgga ctttgaacag gaaaataaaa taccactcaa
     2461 aaaaaggagc aggaatgttt gcaaggattt catgtctgta taattagtgt ttaattaaaa
     2521 cgaactaagg gctatgtaaa aatggagggt ttccttacgg tcccgtatac tccatttgga
     2581 tagttttttg atcgggggcc gtccggtagt tttacataaa ttgtatgatg aaacggattg
     2641 gatacctgct tttacctgcg ctgatggcct tttgtagcgc caatggtcag tttcccggtc
     2701 ttgaccgggt ccgggaagcg atttacgggg ccagcaatga ccaggaacgt ctgagccggt
     2761 tacaggccgc ctccgccttt aaaaattcaa tgcatgggga tacgatctat cattatgcaa
     2821 aatgggccaa ggaactggcc ctgaagctgg gtgataaaaa agcactcgcc cgggcagaat
     2881 acagcctgat cagcggggac atcgccagcg gaaaaacaga cagcgtcatg ccccgcatcg
     2941 accggaataa gattttcatc ggtataaaag aaaccgaccc cgatctgttt tataaaatcc
     3001 agttactaaa agccaatgta ctgaaccgaa ccagccggct gaacgaagcg caggaactgc
     3061 aattgcaatt actccaatcc gcggaaaagg aaaaaaatac tatggcccgg ctctttttat
     3121 tgaattatgt gggcgccact tatctgaata caacgggaca gcgggaaaca gcccggaaaa
     3181 cctgggaaag cggactggac ataatccgac agaccaatga cccggcctac agtgaaattg
     3241 aaacctacct gctttccaat ctatcccttt atttctttgg taactattat gccgccccca
     3301 ctcctgaacg atttgacacc tgcctccgct tcctcaaccg tacaatcgat ctctgccgga
     3361 aaaacgaatc catgggcgta ctggcctcta ctttatcgta ccgggggaat ttatacggcc
     3421 ggaataaaat gtataaagag gccgaagcag attttacagc aacggttgaa atccggaata
     3481 agatcggtga tccgttgtac ataacggagg atttaaaaaa tctttccttt ttctacttcg
     3541 aacgaaatga gctggataaa tgcctggctg tgatcaatga ggggctgctt acttgtgaac
     3601 gatttcatat ccgggaaacg gttcttgatt tactcaaaat gaaagccggg gtctatcaga
     3661 agaaaggaga caaggaacaa tatataacgg tactggaaaa actgttgctg acgggtgata
     3721 cgatcttcca ggtcagaaca gcggaaaaaa tcgccaaact acagaccgaa tttgatgtac
     3781 aaaaaaaaga agccctgatt gctaaacagc aactggatct gctcaaacgg aatttcctcc
     3841 tttatgcggc gggaatcgcc actttcctcc tcgccctgta ttttgtattc cggttcagaa
     3901 aatacaaacg aaaacaacag ctgttgatgg aagaaaagaa acagcaacat gccctccagg
     3961 taaaagaggc ggaagaaaaa gaacgaaaaa gaattgcggc tgaactgcat gataacctgg
     4021 gggtacaggc caatgcgatc ctgtataaca gcagtcagct ttcggcagga acaggaaaca
     4081 cagtacagat tgccaatgac ctgcaggaaa ccgccaagga aatgctgttg aatctgcggg
     4141 aaactttatg ggcaatgaaa aactcggatg taaatgccgt acaactctgg ctgcgggtca
     4201 tcaactttat gcaacagatg ggtcgtcatt ataccgacat ccagtttaaa gtatcaggca
     4261 ccgcacccaa agacttaatc attccggcca accgggccct gcatatcgta ctcgttatcc
     4321 aggaaaccgt caataactcc gtcaaacatg cccgggccac aaccatcatt gcacaaagta
     4381 ttttacagga aacggactgg acgattttac tgcaggatga cggtaccgga tttgacctgg
     4441 aaaaagcaca aacaaaggaa gaccgttatg gtcttaaaaa tatacaggaa cgcgcattgg
     4501 ccggtgaatt cagttgccgg ataagtgctg aaccgggtaa agggaccctg accactttac
     4561 agatcagtca cagccgttag cggtacaacc aatgcctgtc aacattctca attactttct
     4621 tatatttaat tatgatcaga gtagccattg ccgacgataa aatttacaac cggaaggtca
     4681 ttgtggacaa actggcccgt actgcattat tttccctcgt attccaggcg gcggatggca
     4741 atgagttttt gcaaaagatg cgggagctga aacaggaaga actcccgcat attgtactga
     4801 tggacctgga aatgccggaa gtggatggag tatcggctat cggtacggct tcggccctct
     4861 acccttccgt aaaatttgtc gtactgacta ttttcgatga tgatgataaa atattcaggg
     4921 ccatccgggc gggtgcctgc ggttacctgc tcaaggaaga aagcggggaa gtgattaccg
     4981 atatgctgat gaatctctgg gaaaacgggg ccggaccaat ttctcccagc attgcctata
     5041 aaattctgca aatggtgcaa cagccagtgg ctgttactga cgaaaaaaat aaggcagaga
     5101 atatatttca actgagtgac cgtgaactgg aaatcctgca acttttatgt gaaggactgg
     5161 agtataagga aatcggcaac cggattttca tcagtgccaa taccgtaaag aaacattgta
     5221 tcagtatcta tcagaaactg catgtcaaca gtaaggccca ggccctgcgg atcgcctata
     5281 ccaaagggct ggtataaccg ttaaaccata tggcattaat ccagttcccc ggcctgcatc
     5341 atcttgtaaa tcgtactctt tccgatatcc agggcattgg cagctgccac cacatcatta
     5401 ttatgttttt tcaggtattg cttcacaata tcacaggtat actgacgaag tgttttattc
     5461 tccgtgataa ataaatcatc ccgtttgccc tgggaaaaag taatgtcttc ttcccggatc
     5521 tcattgtctt cacacattac caccgccaga tcaatcacgg ctttcaactc ccgcacattt
     5581 cccggaaagg gatggctcat cagtttgtcg cgggcttcct tactgaactg gatggtaccg
     5641 agattatttt ctttggcaaa ttcatccgca aaatgcttcg acaataaaag aatatcgttt
     5701 tcccgctccc gcaacggagg caatacgatg ggcaacccca taatcctgaa atacagatct
     5761 tccctgaaat tccccctctg tacttcctgc gccagattct tatgcgtagc agtgaccaaa
     5821 cggatatcca gttttatttt ttcatttcct ccgaccctca ccacttcccg ctcctgcaat
     5881 acgcgcagca gtttactctg aagactcata tccatctccg caatttcatc cagaaaaata
     5941 gtaccgccat tcgcttcttc aaacttgccg ggttttcggc ctacggcccc ggtaaaagcc
     6001 cctttctcat gtccgaataa ttcactttcg atcagctccc tggggatggc ggccatattc
     6061 acagcaacaa aattcttttt cttccggtca ctgttgtaat gaattgcttt agccactact
     6121 tccttacccg taccggtttc cccattgatg aggatattaa tattggtctt taccgcttta
     6181 ccaatcaggg tgaaaacctt tttcagactt tcactttgac caatcatggt tttctgaaag
     6241 gagaatttct gtcccagttc ttcccgcaac tgttccactt ctttcttcag cgactgcgtt
     6301 tcgcggatac ggatgatact gttccacaac aaatccttgg tggcctcatc ctttacaaga
     6361 taatcgttca cgcccatctt gagcaggtta acggcaacgg ctacctcttc ctgggaactg
     6421 ataactatga cgggtacctg cggactaatc tcacgtatcc gtttaaataa atcatccccg
     6481 gttatatccg gcaaggaaaa atcgatggtg accaggtccg gcttgagatg catcttagcc
     6541 aggcaatcct tccctgattc aaaacgggtc actttatagt caggatttaa agagagatgg
     6601 taatcaagga tctccccgta ccagggatca tcttcaacca tgtaaattgt ataggcactc
     6661 atattagctg gattttaatc tgcttgtact ggtttttcag aaatctatat caatgtacaa
     6721 attttgtttc aattacaata attggataaa attccaataa atggaaatat acgctgagca
     6781 gaataattac aattgattga tttactaaca gatatctgtt ggcatctgct ttgaaatatc
     6841 tgagaaaaac aaactctatg caattaaccg taacaacccc catgaatctg aaatcagccg
     6901 gtcaaaaaat cccgggatgg cagcctaccg attttttaga caaaaaagga atctgatcgt
     6961 caaataagta cttgcactta tcccttgtag tcataaatca ctatttgata aaaatgattc
     7021 atatgattga ttatcatata tacaatacta tagacaatcg gatgtttata ggttattaaa
     7081 aaagaatcct ttctatcctg agaaaaaact aaatcaaaac gtaaaactct aattatgaaa
     7141 attgtaattg taggaagcgg ctatgtcggc ctggttaccg gcgcttgctt ttcagaagtt
     7201 ggtatcaatg taacctgtgt ggacattgac aaaaagaaaa ttgaaaatct taataacggt
     7261 attatcccca tatatgaacc tggactggag gatatgataa accggaatgt gaaaaagcaa
     7321 agactgacat tcaccacaga tatcaaggag gcgattcagg attgtgaggt tgtatttatc
     7381 gcggttggta cccctccgga tgaggatggc agtgccgatc tgaaatatgt actgtctgta
     7441 gcccgtgatt gtggccggaa tatgaatgat tacctgctga ttgttacaaa aagtacagta
     7501 ccggtgggca cttctgaaaa agtgcggaaa gcggcacagg aagaactgga taaaagaaaa
     7561 gtgtccatcg acttcgatgt ggcttccaac cctgaattcc tgaaagaagg cgccgctatt
     7621 gacgatttcc tgaaaccgga ccgcattgtg atcgggctcg aatcaacccg ggcagaagaa
     7681 ctgatgaagt ccctgtacaa accttttacc ctgaacggtc accccctgat tttcatggat
     7741 atcacatccg ctgaaatgac caagtacgcc gctaactcca tgctggccgc taaaatcagt
     7801 ttcatgaacg atatcgccaa tttatgcgag atcgtgggcg cggatatcaa cctggtcaga
     7861 aaaggaatcg gatccgactc ccgtatcggc aacaaattta tttacccggg tatcgggtat
     7921 ggtggttcct gtttccccaa agacgtacag gccctcatcc gtaccgccgg tgaatacaat
     7981 tatgaactgc gggtactcaa agccgtggaa gcggtgaata aagaccagaa aatggtaatc
     8041 tataataaga tcatgaacta tttcgacggg gatatccagg gcaaaaccat tgccatctgg
     8101 ggactttctt tcaaaccgca gaccgatgat atgcgggagg ccccttccct ggtgattatt
     8161 aatatgttgc tcgaagcggg tgctaaggta aaagcctacg atccggtagc gatgaaagaa
     8221 gcccgtcatc attttggaga gacgatctcc tatgtggaag atccgtacga agccctgatt
     8281 gatgcagatt gcctggcgat cctcactgaa tggcctgaat tccgggtacc aaactttaaa
     8341 atcatcaaga aattactgaa caacgcagtc atcttcgacg gtcgtaatat ttatgacaag
     8401 gcagaaatga accgtaacgg atttaactac ttctgcattg gtgtgcaaac cactaccaaa
     8461 caaaagaccg aactcgtata acccaccagg aagcattcct ctcaccagga atgcttcttc
     8521 ttaatctaca atatgaaccg taaaaaaata ctggtaaccg gcggtgccgg tttcctcgga
     8581 tctcacctgt gtgaacgctt actccatgaa gggaatgaag tgtattgcat ggataatttt
     8641 ttcaccgggc agaaacagaa tattgtccac ctgctggaca acccctattt tgaactgatc
     8701 cggcatgatg taaccgcccc gttttttatt gaagctgacg agatttataa cctcgcttgt
     8761 cccgcctccc ccattcacta ccagtacaac ccgatcaaaa ccatgaagac ttccgtcatg
     8821 ggagcgatca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cactaccagt
     8941 acaacccgat caaaaccatg aagacttccg tcatgggagc gatcaatatg ctgggactgg
     9001 cgaaacggat aaaggcaaaa atcctacagg catccaccag cgaagtctat ggcgatccaa
     9061 aaatacatcc gcaaccggaa gcctactggg gcaatgtaaa ttgcataggt gaacgttcct
     9121 gttatgacga agggaaaagg gcggctgaaa ccctgtttat ggattaccat aagcaaaacg
     9181 gagtcaatgt aaaaatcatc cgcatcttca atacctacgg tccgcgtatg caccccaacg
     9241 acggtcgggt ggtctcaaac tttatcgttc aggcgcttca aaacaaagac atcaccatct
     9301 atggcgaggg caaccagacc cggagttttc aatatgtgga tgacctgatc gaaggaatga
     9361 tccgcttaat gaataccccc aaaggattca ctggtcccgt caatatcggc aaccccaatg
     9421 aattcaccat ccgtgaactc gcggagaatg tgatccgttt gaccggatcc acatcaaaaa
     9481 ttgtaaaaat gcccctgccg gccgacgatc cgatgatgcg taaacccgat atcagcctgg
     9541 ctaataaaga actgaactgg aacccgaata cccagctgga ggaaggatta cttaaaacaa
     9601 ttgccttctt taaaaccgtt atctgatttc ctgaaaattg taactacttt ttaaaccctt
     9661 aatagtaaaa gctgccgtct caatacgggc agctttttta ataaaattct ccggcatcaa
     9721 actgaaaagc gattttccgc aatttggaaa atttttcctc aaatgagacc aattgccaaa
     9781 cagagactta tttaatcaac tgatttacat ataactgtac aaatttttat tctggcacag
     9841 gaatggactt attgaagtaa atcaagttta aaaattcatt atgcctgaac aactatacaa
     9901 tctgaacctg gtgaaaaaac tggcgcacaa taacatggat ataatcaata aactgatgca
     9961 cgtatttatt gaacaggctc ccacctccgt gaaagatatt aagtcagcct actactctaa
    10021 aaaatatgca gtagttactg ctctggccca taagataaaa ccaacctatg gttatttcgc
    10081 tgtcaatgaa acggaaaaag acctggaaat gatcgaaaga ctatcgaccc tggaatctcc
    10141 atctgccgaa atagaagaaa tgataaataa agtggaggtc gcaactatga agctggtggc
    10201 tgaaatgaaa gctgacttga actaagtaaa atcacttacc gggaataggc acatttatct
    10261 agttattata taaacatctc tttctaaatc attcaaacac aataaaagcg ttacttactt
    10321 aagtccgcta ccaatcgaaa acaatcgcca aaatcaaaac gacctgtaaa tccatgatta
    10381 tgaaaaaaac aatacttgtc ctagatgatg aatctattat cggactcctg ttagagaatt
    10441 ttctctctgc ccaatacgag gtaaaacaca ttaacaatgg tcagcaagcg cttgaatggc
    10501 ttgaaatcaa tattccggac ctgattatct gtgatattga aatgccggaa atgaatggat
    10561 ttgatttcct gacacaggta cgtcaacgtg gctttacccg ccatacaccg gtggtgatgt
    10621 tatccgcaaa atcagaaagc aaagaacgga tcaagtgcta taaactgggg gcccaggatt
    10681 acctgaccaa accctttaat ccggaagaac tcgaagaaat cgtcaagaaa aatcttttcc
    10741 ccattcactt ttccattgcc tggtaataaa acgaaccctg ctcatgaaaa cttctttatc
    10801 cgtattatat gtcggcaaca accccgctta ctttcaacct cttgaaaata aaggtgaaat
    10861 agctctaaag actgtgtctt cttcagtccg cggcattaaa tacctctccg aaggaaataa
    10921 aaccgatgtc atattcagcg acttttgcct gtccggctcg aatggacttt tcttttttga
    10981 ctggatcaga acacaaccgg gatttgataa agtacctttt atcttattat caaaagaatt
    11041 cagaagtgaa atttacaaaa ccgcttatca gaaaaaagta gacgattatt attcactggc
    11101 acagcctgtt ccggaagata tcagcacccg gcttcgtttc ctggttaact tcaaaaagaa
    11161 aaaaggggtc tatgttgatg aaaagaccag cgccaaattc agtatgcctc tttccaaaag
    11221 gctttttgat attgcggttt ccgccaccgc cctgctcctg ctctctccga tattgcttct
    11281 tgttatcctc gcgatccgac tggaatccaa aggaaaagtg tactatatct ccagacgtgt
    11341 cggccgaaaa ccttttgatt tctataaact gagatccatg cgcaccggtg cagacagcca
    11401 gttaaaagcg ttggctaaaa tcaggaatca gtatgcagat acttcggcca atgcggaagt
    11461 ggattttaca ttgccttgcc cgagatgcag caatatgccg gatggtcagt cctgttctcc
    11521 catactctat attaaagatc acgccatctg tgatttctgg cacaatgagc agcagaaaaa
    11581 tgtagccgct aaaaactccg tatttgtaaa aatcaaagac gatccccgcg ttacccgtgt
    11641 gggtaagttt atccggaaca ccagtattga cgaattaccc cagctcatca atgtgctcaa
    11701 aggagatatg tccattgtag gcaatcgccc tttaccggtg tacgaagcag aacaactgac
    11761 ccgggatgat ttgtccaaac gttttctggc gcccgcaggc atcactggtt tatggcaggt
    11821 agaactgaga ggcaagggcg gcgtgatgtc ggaagatgaa cgcaaacgcc tcgacaatga
    11881 atacgccgac catttcaaag acggaaaata ttctttctgg tatgatgtta aactgatgct
    11941 tcgtaccatc cccgccatgt tccagaaaga ctctgtataa tttcttttaa tccaccaaaa
    12001 tgaaactcac tgccatttac ataattacct tactgctgat cctctccgcc agaccaggtc
    12061 aggcacagtc tgccgattca acggcaatga ccccaagcga cagtgtaatt acttttcctc
    12121 cgctgaatga cctcattgat tccgctgtaa aatatcatcc gggtatccgc taccgccagt
    12181 atgaagtgaa agtaaaagaa tcaaatctgc aaacccagca acacaactgg acgcggaata
    12241 tgggtgtaca ggcggacagc cgctatggta cgtttgacaa tttctccgcc aatgataacg
    12301 gacagtcaac gaccctgttg aatacaacca acaaacagtt taattacgga tttggggtat
    12361 atatgaagct tcccctttat gatctcctca accggaagaa ccagttaaaa caagcgaaga
    12421 atgaagtgga aatggcccgt agcctcggtg aagtccagga aactgaaatc cggcagatgg
    12481 tgattaaaca gtaccaggac gccctgctac ggcaacggct gatccggatc aaagcgcaga
    12541 acctgggtaa tgcccgggtg aatatggaaa tggtggaaaa agaattccgg aacggcatca
    12601 ttcccatcag tgaatatgcc cgtatctctg atattgttta ccgggccgaa tctgattatg
    12661 aactcgccaa aactgaattt ataacggcca aactattact ggaagaaatg gttggattca
    12721 aattttaata aaggaccgtt caaatgaaaa tcatagaact gatacgatta atccggaaac
    12781 acctggtact gctgattacg gtatcggtac tggccgccgt actggtgacc atgctgaccc
    12841 gtaatcccaa actcactttt gcttccaaga ccaccttata tacaggtttg gccaccggct
    12901 ccaccgtaga aatggataaa acctacaact atgcgctgac caatacctcc tttgacaacc
    12961 tgatcaatat cattaaatca agagaaaccc agcaaaatgt ggcgatccgc ctgctgagcc
    13021 agcacctgct gcttcccggg cccgacccga aatacatttc caaacaatcc tgggaggaac
    13081 tgaaaagaat tacacccgaa tacatttaca actatgtggt aaaaagcaaa cccaccaaca
    13141 caatcaccac cgataccata ccggtaaaaa caaaaaacaa aggcgacagt ctgcgtacac
    13201 ggagcgccaa cacccattcc gctgattctt cccagattct ctataaatca ggattgttcc
    13261 cggcatcggt cagccggctt gatttcgaaa aaaccgtata caacctgacc cagctgatgc
    13321 acagcagtga caccaacttt gtatataaac tgctgaacta tactgaccca cactattcca
    13381 ttgccgccat ttctggtgta aaggccgaac ggattgccag cagcgacctc gttcgcctca
    13441 gctatgaaac ggatgatccg ggtatctgcc agcagaccct cgcgatctac tctgaagtgt
    13501 cgatcaacaa tttcaagaac atcaaagaaa accgatctga cgctgtagtg aaatattttg
    13561 aatcacagct gaaacaggcc gatgacaaac ttaaagaagc ggaagacgat ctgctcgctt
    13621 ttaataaatc caataatatc atcaactact acgaacagag taaagcagtt gccgtggtaa
    13681 aagaggatat ggaagtggat tataataaca aaaaagccca gttggctggt attgaagccg
    13741 ccacacgcag actggaagac aaactgaata tccagcaggt tgtgcagctg aaaagcggcg
    13801 tagtcatgga taaaaagaaa aggctgagcg aagtgaatta cctgatcgcc gaggcagaag
    13861 cagataataa agcggacggc ccgttaaaag cactgcggaa tgaagccaat gtactgaaag
    13921 aagaaatccg gaaaaatgta ggggatcttt acgcttatca gaataccacg gatggattgc
    13981 cggtaagtaa agtactcaat gaatggatca acagcgtggt ggaggccgat aacctgaggg
    14041 ccagtatccg ggtgatggat cagcgtaacc gtgattttca acaacagtac tccatctacg
    14101 cccctgccgg cgcacatatc aaacgaattg aacgggagat cgccgtatct gaacaaggat
    14161 acctggaaat tctgcacgga ttaaatctgg ccaaacttaa actccaggac aatgaactat
    14221 cctccaacct gaaagcagta gatctgccct tttatccatt aacccccacc cccaccaaac
    14281 gtaaagtgct gatcattgcg ggtgcacttg tatcagctat gctggtgctg ggtttgatac
    14341 tggtgctgga gtattttgac gaaacactga agaacttaaa aagagcggca aagacattac
    14401 agctcccggc gctgggcatc attccgaaag tctacctgca tcctgaaacc agcaatttcc
    14461 cttatatcca aaaccgctta ctggagttct ccatacaaaa tatggaacag tttttcggct
    14521 tgaataacgg gtcaaagatt actaaaacca tcctctttgt cagtacacag gaaaatgaag
    14581 ggaaaaccac cctggcaggc aacctggccc tgaaactgac ccggcagggt aagaaagtca
    14641 ttgtactaaa ctataaccac cagccgaaaa acacttcaaa gcattcaaaa tttccgattt
    14701 tcagccggct actcggatac agtgaccccc ggattgacta taacagtccc tttctggcag
    14761 cgcccgaaaa ttatttgccc aaagaaaatt gtttcactta cacagtcaat gatgattttt
    14821 ataacagccg gtcttatacc gaaattctcg aacataacgg acaacaggcg gcttttgtac
    14881 ccgactatgt attaatcgaa cttccggccc tgctctccaa aaattatccg actgagctga
    14941 tgtcgatgag cgatctcgtg gtgctggtat gccgggccaa ccggatctgg acagaatctg
    15001 atcagctggt gatcaatagc attcagccat taaccggcaa caaacttcat ttcattatta
    15061 acggatcaca actgaaagaa gttgaagcgg tgatcggcga tcttcctaaa aaaagaagca
    15121 tccttcgtaa aaaacttaaa aacctgttcc gcttccagtt cttttcaagc aaccgtattt
    15181 aattaaccga attgaaaaaa accctcaata aaatcatccg ggaagacaac ttcctttctt
    15241 tagccggaaa cctggtgatc gctgcggccg gtctggctgg ctttgccctg ctggcccgta
    15301 gtcttcagct gaatcagttt ggagaatgga tgctttttat ttcttcgggt tcctttatag
    15361 aaatgttccg cttcggtatc accaataccg gactggtgcg ttacctatcc ggtgcggatg
    15421 aacacacccg cataaaactt atcggttcca attcggtcat cagcctcatc agctcgctgg
    15481 tgattattgc cctccttgtt atctgcaatc tgctcttcca tacttcactg gaaaaagcag
    15541 gttacggatt gttctttacc tggtatcccc tgctggccct ggtgaacctg ccctggaaca
    15601 atgccctggt ggtactgcag gccgaccgga aatatggaaa gatgctgctc atcaaatcca
    15661 tgaacagcct tccgttttta atggtggttc tggttaatta tttcaccggc tattttacat
    15721 tgacacaact ggccattgcc ttactgatca tcaatgccct gacctccctg atcagtctgg
    15781 ctgccggatg ggatggactt cgctatatat tcaaagcaac ccgtgcttcc accaataccc
    15841 tgctgcattt tggaaagtat accactttta cccttgtagg aaccaacctg ctgcgcagtg
    15901 ccgattccat tatcatcagc ctaagcccga tgggagtaaa tgccgttgcc ttgtacagta
    15961 ttccgcttaa actgaccgaa ctgcaacaaa tacccctgcg cagttttgcc gccaccgctt
    16021 tccctaaaat gtcaaaagcc agtatccatg gaaaactgga tgaactgaaa aaattatttt
    16081 atacctacac cggagcgatt acttatctgt tcgttggagt cagcctgatt acgtttgtgt
    16141 ttgcagattt ctttgtactg gtattggccg gcaagtcctt cctgcaaaac aatccggatg
    16201 ccggtcaggc catcagcatt gtccgcatct tctccttata tggattactc ttgccgctgg
    16261 atcgtatgac cggtatcggt ctggacagta tcaacaaacc gggagtcaat gcgcttaaag
    16321 tactgatcat ggtgctggtt aatatagcag gtgacctgat cgctgttttc gtgtttcaat
    16381 ccctgttgct ggtagcgatc tcttccattc tgtttaccgc catgggcatc tggctgggta
    16441 tgtatttcct gaataaagaa ctccacctgc gctaccggga tatattcacc gaaggggcgt
    16501 ggttttataa aaacatttta aacaaagttt ccggcaaccg gctggcggta aatctcaacc
    16561 ggtaatcctg caatccaatg acaactggtc agcatatcaa cccttttgaa tataacaccg
    16621 gctcttcccg tctgtcgagt ccgcgtgctg tgctgctggt gataatgatc atctcaggac
    16681 ttgcatttct ttcggcaaaa ttgggaatga tgagctttgc cgtcagtgtc ggcttacccc
    16741 tgttcctggt gtatggctat ttccttttta cctggccggt cgttggttta tataccgcca
    16801 ttgccatgag ttttgtactg atcggagccg gacgatatgt gaaggatgtg ccggtcggtc
    16861 ttggactgga tgctgtactg atccttacgt ttattgcatt attcattaat aagtttaaag
    16921 aaaaagtaga ttggtcaccg gtcaataagg atatcactta cctgtcattg atctggtttg
    16981 gctattcaat gtttcaactc gtgaacccgg aatcccaaag tgtggcagcc tggttttccg
    17041 gacgtggcgt ggccttgtac atgtttttga tggtgcccct tacgttgctg ctcatcaata
    17101 ccaaccggaa gctggatatc ctgctctatg tgtggggagc cctttccatt ttagccacat
    17161 tgaaaggaat tatgcagtta aagatcggac tggatgcggc tgaacagggc tggctggatg
    17221 acggcaatgc gaaaacgcac gtgcttttcg gaaaactcag ggtcttctca ttcatgagtg
    17281 atgccggaca gttcggaggc aaccaggcgt attccgcagt agtgtcattt attttctcca
    17341 tggcacaaac ggatcgtaaa aagaaatggt tcttcctcat cgtttcttta ctggcttttt
    17401 atggtatgat catttccgga acccgtggtg ccatcagtgt acccctggcc ggcttcatgc
    17461 tcttctttat cctccgcaaa aatatccgcg tattgagtat cggtgtggca atcctcgctg
    17521 ttgtgtttgt cttctttaaa tataccacga tcggacaggg caatgaccag atccgccgga
    17581 tgagaacagc cttcgacccc aatgacgcct ccctacaggt tcgactggat aaccaaaaat
    17641 tactcaaacg ctacctggct tcacgcccct ttggaggtgg tatcggtcac ggtggggtaa
    17701 aagcgcaaaa gtatctgccc aacgcatttc tctcgcaaat cgccaccgat agctggtatg
    17761 tactcgtatg ggtggaaatg ggaataatcg gcttattact gcacctgttt atacttttct
    17821 atacactgat ccggggcacc tggctggtga tgttccggct gaaggatcca aaactgaaac
    17881 tgaaaatcag tgcgctgatt gccggtctgg caggtgtaat ggtagcttct tatggaaacg
    17941 ctgtattagg cgccatgccc accggaatgc tgatctatac ctccatggca attatactga
    18001 atgcaaaggc gcttgataaa agtacaggag aacctgtacc ggtattaata aaataaaaac
    18061 cgaacattaa atcccctatg atatgacatc aacaatgact gaaaaaatga aaggcaagga
    18121 ttttatcatc accggactgc agtcctggga tataccgatc ggatccaatg ctatcgatat
    18181 tgctaaagaa atagcaaaaa ataaccgcgt gctgtatgtc aacagtccgc tggatttaat
    18241 gacgatgtac cggaataaga aaaccccgga aaatgaacat cgtcttgatg tagtgaaaaa
    18301 attcaaaaaa ccgatccgcc agatcagtga aaacctctgg gtactcgatt tcccttttgc
    18361 ggtctggtca gtcaatggtt tcccggatgg ccccgtcttt gacctgttca accgccggaa
    18421 caataaacgg attttcaatt atgtgaaaaa aactgccgca gaactgggtt tcgaaaatcc
    18481 ggtacatttt attgacaatg atatctaccg tagtttctat gccaaagaga tattaaaacc
    18541 ccgcctctcc gtctattacc gccgggataa cctgcagccc tttgcctact ggaaaaaaca
    18601 cgtggcccgg ctggaacctg ccctgatcgg caaaagtgac ctggtggttt gtaattctcc
    18661 cgaactcgca gatttctcca ggcaattcaa tcagaagagt tttgatgtag gccagggcgt
    18721 ggacctttcc gcgtatgatc cggataaaaa atttccgatc cccgcagaac tggccaccat
    18781 acccggaccc atcatcggtt atattggtga tatcaacagt ctgcgtctgg atcccgattt
    18841 gatttatgaa ttggcgaaag cccgtccgca atattcattt gtaatgaccg gccgtgaaga
    18901 caaggtattt gccgcgcatg ccctgcacac cctgaagaat gtacatttta ccggcagtat
    18961 ccccaaggac agggtgcctg agtttatggc cgccttccag gtatgcctga atccgcaact
    19021 gatcaacgag atcacaatcg gcaactatcc gcgtaaagta gatgaatacc tcgccatggg
    19081 taaaccggtg gtagccacga ccacgaacac catgcagctt ttcaaagaac atgtatacct
    19141 gtgcaaaaac gcgggtcagt atatggtagc ggttgataaa gcattgtccg aagacaatga
    19201 ccggttacgt cagcagcgta ttgcattcgc ccgttcacat tcctgggaaa acaatgtcca
    19261 gaatatctat aaccagattc agcaatcctt aaactaatca ctatgaacta cagaaatatt
    19321 gccgacctca ataaagtcat cctgaaaaaa ctgagcataa tcccccggga tatcgacctg
    19381 atcgtaggtg taccccgcag cggtatgatg cccgccaacc tgctggccct ttacctcaat
    19441 aaaccgtata ccgacctcca ttcctttatc aatggacata tttataaagc gggtgcccgg
    19501 gcccagttct ttgatgccaa cagcgggagt aagaaaatcc tggtagtgga cgacagtgtg
    19561 gcttccggac gtgccttaaa tgaagtaaag gagctgctga aagatcacag cacccgtttt
    19621 gatataaaat actgcgctgt gtatatgagt ccgggcaaag agaaaaccgt ggattacttt
    19681 tttgaaacgg ttgccctgcc ccgctatttc caatggaata tcatgaacca tagctcactg
    19741 gaaaaagcct gctttgacat cgatggggta ttatgtgagg atccgaaacc cgaacagaat
    19801 gatgatggtg aaaagtatac cgactttatc ctgaatgcac cacccctgta cattcccggc
    19861 agcaaaatcg gcaccatcgt tacttcccgt ctggagaaat accggaaaga aacagagatc
    19921 tggttgcaga agtataatat aaaatacaat gaactgatca tgcttgacct gccggatatg
    19981 aaagcaagac aggaagccaa taaccatggc ggacataaag ccaatgctta cctgtcaaaa
    20041 ccgtacaccc tttttgtcga aagcgaactg ggacaggcca gggagatcaa ccggcttacc
    20101 aaaaagccgg tattctgtac cgaaactttt gaaatgattt ttgaatccga gtcggtattg
    20161 tacaacctca aaagcggcaa atacctgccc ttcctgcgca agtatgcatt aaaagcccgg
    20221 accaaaatca ggaaaatgaa aaatgcccgt cacgcataaa aaaccagaag acccgaaacg
    20281 accactttta actcaattat catgttagtt tataatatca tacaggtcat actacttagt
    20341 ttactcggat cagccaccct gtatattttc atatttgccg tggccggcgt attctataag
    20401 gaaaagaaat ccgcaaaagc cgacaggcac cggaaattcg ccgtgctgat tcccggttat
    20461 aaggaggatg aagtcattgt ggaagtggcc aaatcggcct tactgcagga gtaccccatg
    20521 ggtctttttg atgtggtggt gattgccgat tcatttcaac ccgaaaccct ggctgaactg
    20581 agaaccctgc ctgtaaaact gattgaagtc agttttgaca agagcaccaa atccaaagcc
    20641 ctgaacaaag ggatggccac acttacttcc cactatgata ttgcagtgat cctcgatgcg
    20701 gataatatca tggcccctga ttacctgctg aagatgaatg cggcctttgc acagggtgct
    20761 tttgctgtac agggtcatcg taccgccaaa aaccagaata attcctgggc ggtactggat
    20821 gcggcgagtg aagaaattaa caaccatatt ttccgtaaag gccaccgcgt actgggactc
    20881 tcttcggcta ttatcggatc cggtatggct ttttattatg actttttcaa aaccatgatg
    20941 gccgatgtaa aagccgttgg tgggtttgat aaggaaattg aactgaaaat gctcaaagcc
    21001 aaacaccgga tcagttacca ggatgacgcg atgatctatg acgaaaaagt acagaaagcc
    21061 gatgtgtttg gcaatcaacg gagacgctgg ctgtctgcac aaattcatta tttccaaaaa
    21121 gatattattc cggcaatcgg tcatctcttt acaaaaggca acctggatta ttttgacaaa
    21181 gcaattcagt ttatccaacc acccagggta ttactcattg gtgccttatt gctgttgagc
    21241 accctcttta tcggggccaa ttacctgctg aataacggca ttagctattc caccgcctgg
    21301 ctcgccatca ccgccgcctg tatgctggcc ttactgttgt ctgtaccccg ttccttttac
    21361 aattggaaaa cgggtcgggc attgatcagt ctgcccagcg ggatgctggt gatgcttatg
    21421 tcgctgttga aaaccaaagg cgctaacaaa acatttattc ataccaagca tacgagcagc
    21481 agcagataaa gcagtatttg taaaattttt acctatgaaa aagaacagac tagaaaaact
    21541 ggaagcgctg cggggatttg cggcgcttta tgtggttttg tttcatgtgc tgccccagca
    21601 aatcctgctc ttcggcgtca atatcgggct cctcttccgc ttcggaccgg aagcggttat
    21661 cgtattcttt gtgctgtccg gcttcgtgat taaatacact tttgaaagat cagctgataa
    21721 atccttcaaa tattatttta tccggcgttt tatccggctc tatattcccc tcttctttat
    21781 ctatgtactg ggttacctgt tgaaatgtta caatgccggc gaactggcca atccggaatg
    21841 gggcagcctg ctgggtaatt tattcatgtt gcaggatgtg atttcccaaa aacccaatgt
    21901 gatatctgcc gcctatatgg gcaacggggt actttggtcc ctttcttatg agtggtggtt
    21961 ctatatgctt tactatgtac tggctacccg tataccggcg gaaaaactga acaaatgggt
    22021 gaatattatc gtcatcaccg cggccgcctc ctacctcatt tatcctttta ttgtgaaccg
    22081 gctgatcatg tatttcgcca tctggtggat tggggtacgt tttgccgaaa tctatcttca
    22141 gggcggcgcc ttctctttta aaaaagttat gccttatggc gctgtattgc tggccatcac
    22201 ttgtattctc ggcctgaatc tctaccttaa tttttcctat accaaagtgt acagctaccc
    22261 gctggtggcg tatcccttta tcgaagtccg gcattttgta tttgccatca tcgtcatgtt
    22321 tggcgcgatt gcctggtata acctgaaatg ggtgggattc aatgccgc
//
LOCUS       DAOPMW010000017        22206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2187_length_22206_cov_10.120717, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000017 DAOPMW010000000
VERSION     DAOPMW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 22206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22206
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_22206_cov_10.120717"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1063
                     /locus_tag="PLU11_01835"
     CDS             47..1063
                     /locus_tag="PLU11_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPN57874.1"
                     /translation="MKKVCCFGELLLRLPPANGGEWLRHNQLPVFVGGAELNVATALA
                     TWQLPVKYCTALPDNIISKDIRDYLEQKHIDTSSIILSGERIGLYYLKEGADMKSHEN
                     VFDRKYSSFSTLGTGIVDWETIFSDVSWFHFSAIAPAVSESAAALCKEALEEAKKRGI
                     TISVDLNFRSLLWKYGKQPVEIMPELVQYCDLVMGNIWAANTLLGIPLNEELIAAKSY
                     TAHARFTASEIFTRFPQCNWMANTFRFDGNAEEIRYFATLDNRTEQVVSPLLQTDSVV
                     DKVGSGDCFMAGLIYGLISGNPADEIISFAAAAAFAKLQEKGDATQNSVEKIKAYLQN
                     PVFN"
     gene            1100..>2037
                     /locus_tag="PLU11_01840"
     CDS             1100..>2037
                     /locus_tag="PLU11_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPN57875.1"
                     /translation="MSKKVVTLGEIMLRLSTPDFKRFVQADTFDITYGGGEANVAAAL
                     CNYGLNGTFVTKVPNNPIGQSAINHLRRYGVDTQFVARGGERLGIYFLETGASMRASQ
                     VVYDRAGASIADVNLDEFDWDKIFDGADWFHTTGITPALSDKAAALAEAALKAAKAKG
                     ITTSIDLNYRKKLWSKEKAREVMTRLCYFVDVCIGNEEDADTTLGFKAGETDVTKGEL
                     HLDGYKDVFQQMKAKFGFKFIASSLRESHSASDNGWSALVYDGTDFYHTKKYNVRIVD
                     RVGSGDSFASGLIYGLVTGMSMPDAAEFGVAASALK"
     assembly_gap    2038..2137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2138..2571
                     /locus_tag="PLU11_01845"
     CDS             <2138..2571
                     /locus_tag="PLU11_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPN57876.1"
                     /translation="ANAPKYFSVHPLFEKAFAFISSTDLANAPDGKSEISEGLKAIFS
                     NAQGKTAEASLSKFECHNKNIDIQLCISGVETIGWTPREKCVTPNGGYNDEKDVQLFH
                     DKADTFFQLTDGQFAIFFPEDVHAPMISEGVVKKLVIKVKI"
     gene            2802..3464
                     /locus_tag="PLU11_01850"
     CDS             2802..3464
                     /locus_tag="PLU11_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPN57877.1"
                     /translation="MSNTKKITDAIIAQGILPLYFNPSEEISLDTLKAIYRAGIKAVE
                     YTNRGEAALSNFKKMVELRNAEMPGLLLGVGTIKNMGHATDYMAAGADFLVSPGFVPD
                     VAAYCVANDIFYAPGCMTPTEIIAAENAGIGFIKLFPGNMLGTEFLTTIKDIFPKLLF
                     MPTGGVDTTKESIESWYKAGVCAVGMGSKLISKKLMEAKDYATMEGETKKVLELISSI
                     KP"
     gene            3499..>3873
                     /locus_tag="PLU11_01855"
     CDS             3499..>3873
                     /locus_tag="PLU11_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN57878.1"
                     /translation="MSNYRWVIVSMLFFATTINYLDRQVLSLTWKDFIVPEFHWNNND
                     YGTITAIFSLAYAFSMLLAGKFVDWMDTKKGFLWAIGVWSIGACLHAYCGIATSGILT
                     GTWLVGFEGAKDTIATVSDVAKI"
     assembly_gap    3874..3883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3884..4931
                     /locus_tag="PLU11_01860"
     CDS             <3884..4931
                     /locus_tag="PLU11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN57879.1"
                     /translation="ITSLSVTLFIFARIVLALGEAGNFPAAIKATAEYFPKKARGFAT
                     SIFNAGATIGALIAPISIPFIAKAYGWEMSFIIIGALGFVWMGFWVFIYKKPEKHPKV
                     NSEELAYIQQDDKHANAEEMLAHTKKISFAKAFSYKQTWAFAIGKFMTDGVWWFYLFW
                     TPAYLSSVYHMDSTQSAPQIFTLYFITLLSIIGGWLPTYFVEKKGMNPYAGRMKAMLI
                     FAFFPLLALFAQPLGYLSVWYPVIIIGIAGAAHQAWSANIFSTIGDMFPRSAIATITG
                     IGGMAGGIGSFLINKGSGKLFDHAANTNMKLMGFEGIEAGYFIIFTICAVAYLIGWVI
                     MKSLVPKYKPITDL"
     gene            5259..5678
                     /locus_tag="PLU11_01865"
     CDS             5259..5678
                     /locus_tag="PLU11_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57880.1"
                     /translation="MKFQTRLINFLLPFAAYLLFAFQWFNTYVKAWYANIPEETQYLR
                     GALTNPYIYYTWLFGITSLLHLTIGIVYLVREKTTRPLIYFLMLNLLFTSYYVFLFAA
                     DFPKWSRTGPLNFVILCLLGISIVRYYFTLYRREDRE"
     gene            6202..7206
                     /locus_tag="PLU11_01870"
     CDS             6202..7206
                     /locus_tag="PLU11_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57881.1"
                     /translation="MKLPSILFLLFLLKLTSEAQDSLRKVNILAPDSKTVFIGELGQS
                     LGSFLTVQGVVVDGPDKGYEEGPNLIVHMINDSATQVPLQIPLSPYFGSFGKGTMPVI
                     KTGNSYRLRVYETGEFIGSPDGAFNEAGVVIQTTGFYFKNRLIVLAGEKTAPLEWHPA
                     QFEGREALLTGIAENENDTACIRGASWVLKLVNVPKWTPAEIGKQAEVYGKIQGTPAK
                     NQFYVLQGRSRLIRLEDQLGKQVSLRGLAISQNQYWWFNYRGTDIYVEKMEQLPGWSD
                     ENHFMAMEITGILDQEKLPRLDQIAVKDNRDLKMYYIIRNAKWTPVNKLLEPEWFEFR
                     "
     gene            7267..7653
                     /locus_tag="PLU11_01875"
     CDS             7267..7653
                     /locus_tag="PLU11_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPN57882.1"
                     /translation="MNKDTLAYNNAQSVTEKPICQLLASTINAALPEAENKIWHAIPV
                     WFLDGNPVVGYSKLKNGIRLLFWSGQTFEEAGLKNEGKFKAAEIRYTALDQVNTKDLQ
                     RWLKKARDIQWDYKNIVKRKGQLERL"
     gene            7749..8468
                     /locus_tag="PLU11_01880"
     CDS             7749..8468
                     /locus_tag="PLU11_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57883.1"
                     /translation="MRICSALLLLIFLTGCFKRNESNVNAPCTDSCMVFNIRVTTGLH
                     STTTMANIPLELGWSKPATPLGDPGRLIAKGKTQGNGTISFSFKAKAKELQGGKFYIT
                     ALAGNDYFRTQNEYYGIGKYDSLVNTAIHVPAKATLKIIYKNFNPVSTEDYFQCLPYF
                     LSYGSTGHPIEMKKPDGQLSNTHFFTSYGPFTTLELTGTTAGDQYTYLDIIKKKNGVR
                     VDIRDSIYIVKGETKTYEVSF"
     gene            complement(8759..10084)
                     /locus_tag="PLU11_01885"
     CDS             complement(8759..10084)
                     /locus_tag="PLU11_01885"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HPN57884.1"
                     /translation="MGIIKDRFRAKADSVAAEIKDLLKEHGNKVIGEVTLSQIYQGMR
                     GITGLVTETSLLDAQEGIRFRGHSIPELQAKLPKAPGGSEPLPEGLFYLMLIGELPTD
                     EDAEHVTNILQRRSHVPTHVFEAIDALPITAHPMTMFVVGVMALQTESYFQKKYGEGI
                     SKKDYWLPVFDDSIVLLARLPRIAAYIYRRKYKNSDHIQPNGLLDWAGNLAHMLGYDD
                     ESFRELMRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQ
                     EVIKWVFEMQEELNTDLPTKEQIAEYVKKTLSEGKVVPGYGHAVLRKTDPRFTAQMEF
                     GKKHMPDDKLVQTIWNVYETVPPILQTIGKIKNPWPNVDAHSGALLVHYGMVEYEFYT
                     VLFAVSRALGVLASLCWDRALGFPLERPKSVTTDLVKQWLDGKDNIWGE"
     gene            complement(10315..11412)
                     /locus_tag="PLU11_01890"
     CDS             complement(10315..11412)
                     /locus_tag="PLU11_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPN57885.1"
                     /translation="MHKLDWYILKKFLVTFIFCMLLFTVIAVAVDSSEKADDFVTAGF
                     STKEIINKYYLGFVPWIWSLLFPLFVFIAVIFFTSRMATRSEIIAILASGTSYNRFLR
                     PYFIGGFFLAAVLWVGNSYWIPKANTIKANFQSKYIDSKDPSKGRTFSSCARCYYLRI
                     DTNTFIGIRDYDTTTKFARGFFLERVKENKVVYNLRAESISWDTLTKKWKLFRVGERH
                     LDSVGERLVNRDTMILSMNLKPEELRKDDYLKDKLTSPELAYYIKREEMRGTEGLKTL
                     QVEWYRRTAVSATVLLLTMIGAVIASRKIRGGSGMHLAIGFVIAAVFIISDRFSTVFA
                     TKGNFHPFLAAWLPNIVFLFVAVWLYRKTPK"
     gene            complement(11463..12602)
                     /gene="tgt"
                     /locus_tag="PLU11_01895"
     CDS             complement(11463..12602)
                     /gene="tgt"
                     /locus_tag="PLU11_01895"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPN57886.1"
                     /translation="MAALHFELSHRDTDSKARAGKITTDHGEIQTPIFMPVGTVGSVK
                     AVTQQQLVNEVKAQIILGNTYHLYLRPGLDVLEKAGGLHRFNGWERPILTDSGGYQVF
                     SLAGTRKIKEEGVTFQSHIDGSKHLFAPENVMDIQRSIGGDIIMAFDECPPGGSEFAY
                     AKKSMELTHRWLDRCFTRFNETEPKYGYTQNLFPIVQGGTFKDLRKQSCEYIASKEAN
                     GNAIGGLSVGEPEPVMYEICDWCCDHLPQDKPRYLMGVGTPWNILECISMGVDMFDCV
                     MPTRNGRNAMLFTSNGVINIDNKKWEFDFSPIDESIDCEISAYYSKSYLRHLMKAKEI
                     LGLTIASVHNLAFYLWLVGEARKHIQQGDFAAWKAEIIPRLKQKL"
     gene            complement(12686..15019)
                     /locus_tag="PLU11_01900"
     CDS             complement(12686..15019)
                     /locus_tag="PLU11_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57887.1"
                     /translation="MKKQAVFPLICLLFIHNHVFSQGSAERIFVQTDRDYYAPGETIS
                     FNGFVLSAIDSVSSRNLFVELWDDSLRQWSSITLPLIDGVGAGAVTIPRGLKSTPFFI
                     RAYSDVTARQTQPYQFVKTLYRSAMDVPVAKELNGDGGPVFFPEGGSLVNQALNHVVF
                     KAGSEFRGSVRNSKGETLTTLNPVFNGMGRFTFTPVKGETYTCYWEGGGRTGSTPLPD
                     AVDIGVALHIRQSPDTMFFDLDNGGSPDLLAHQLKMLLMIGNEPAYVVEMNLAKKSKF
                     SYFIPLQDYRSGMAELRVLATDNTVLATRPVFINRHSLTGQPKLELVKKDLSPRGENR
                     VKLQFADTTIRVLSVSLIDAGYAENKGGSGLAATLLSPDGQPVPRAFRAISTPEQMDL
                     AVQVAFSQQPVRDKVPAATVLPKGNYLQLEGTVIKGKKPLAEKNLLVGIRSASNGKEL
                     YKVTTDNKGKFVIDDLVLYGDVYVHCRIPGNELQELTCTFRLNIPSSNQPADLLAAFE
                     QLVRRTAAAYSAGGASPSSSLTDAVDTLAFTEKSITLSEVVLSANSSVQVTKRLKALE
                     EKYIAGSVVSGYSATSETLDVLNDPGRMRYMDIFSYMRANLKRVDVRFVNGARQLIYF
                     GRGLTGEMMVDQFYVDNSIVDRNQLDFISLDQIAAIKFVQNLSIEKTLPPALAIFLKK
                     PGDEGYWEKDRFQILEQKLTGYPLSPEFREPDYRKEEIKVEKDLRKTISWKPNTPLEK
                     GTTEIQFYNSDRTKKIRIIAEGITADGSIVYVEKEIE"
     gene            15191..16351
                     /locus_tag="PLU11_01905"
     CDS             15191..16351
                     /locus_tag="PLU11_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN57888.1"
                     /translation="MLALARHLFTFAAMPPIAWEEILFYFFIALTLIQLFYYGRFFSR
                     VAFFKPKEQQQSRQHPVSVIICARDEDENLARNLPGVLVQQYSSSHELVVVNDNSLDD
                     SKYILQELKKTFKSLHVVELTQEAKLISGKKYPLSIGIREAKYEVLLLTDADCVPASE
                     HWIQKMQEAYTGGIEIVLGYGAYHRRRGLLNKLIRFETFHTALQYLSYALSGIPYMGV
                     GRNLSYKKDLFLRNKGFASINHIPSGDDDLFINKVATKENTAVVIDPDAITRSIPKTT
                     WGGWLKQKSRHYTTAKFYKPKHKFLLGLYFGSQFLYYPAFAAAAIFFDWRFVLPVFGL
                     RLILQGIVLYKSMKKMGEADLWPWFIFLDMWMFFYYLIFAPALWRRPRRSWN"
     gene            16363..17004
                     /gene="rsmG"
                     /locus_tag="PLU11_01910"
     CDS             16363..17004
                     /gene="rsmG"
                     /locus_tag="PLU11_01910"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPN57889.1"
                     /translation="MQHIDILYKYFTEFTPQQLEQWQLLEGLYKEWNEKINVISRKDI
                     DGLYEKHILHSLSIAASFEFEPGMEIIDIGTGGGFPGIPLAIFFPEVKFLLADSIAKK
                     LKVVEAVAAGIGLTNISTRHTRAEEIKNQKFDFAVSRAVAPLKELWSWARPLLKTKPM
                     SPDAQHKPGLICLKGGDLAKEIQESSTRPHMIEIKELFPEEFFQEKYLLYVPR"
     gene            17059..17706
                     /locus_tag="PLU11_01915"
     CDS             17059..17706
                     /locus_tag="PLU11_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57890.1"
                     /translation="MRQITVFIVDDNKELRNALEEIVSMSEGYHCIGTASSAEEAVDR
                     IPIIRPDVVLMDINLGTEESGIDVVRTLKPRIPDTNFMICTVYEEDEKIFEALSAGAS
                     GYILKKTPPGRMLEAIRELYEGGAPMSSQIARKVVAAFQHQQSQHSFTDPEKLNVLTP
                     REMEILELLSKGLMYKEIATQTFLSPETVRKHVYHIYEKLHVTNRVAAVNKYYGR"
     gene            complement(17937..19778)
                     /locus_tag="PLU11_01920"
     CDS             complement(17937..19778)
                     /locus_tag="PLU11_01920"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN57891.1"
                     /translation="MNKQVFFSLITVLALQTAVAQPDRWQQKVKYVMNIDMNVQTNQF
                     TGKQKLEYWNNSPDTLTRVFYHLYFNAFQPGSMMDTRSRRQGTITAGRGADWDMRVKD
                     RIQKLKLDEIGYQEILSLKMNGRVQQFLMDETILEVRLDKPILPRSKVVFDMEFKAQV
                     PLQIRRSGRDNPSSKVRFSMSQWYPKLCEYDYEGWHPTPYVGREFYGVWGDYDVSISI
                     DKKYILGGTGYLQNPNQIGYGYEAPGAKVVRPAGDKLTWRFLAPNVHDFMWAADPDFI
                     HKSVKARDSLTFHLLYKVSNVQAAAWEQILDDAQRALPFMEKKFGTYPYRQFSFVHGG
                     DGGMEYPMATLLIGPGAWLHEWLHNWYYGILGTNESLYGWMDEGFTSYAEDLVTAYLD
                     NSKGFAFDGNYRGYQSLAKSGKEEPLTTHADHFNTNSAYGAAAYSKGAVFLEQLGYIT
                     GASVRDRILIEYYNKWKFKHPNASDFIRIAEKVSDMKLDWYKEYWVNSTKTIDYSIDS
                     LWEEGGKTKVRLNRAGLMPMPIDLQISFKDGSKELHYIPLNLMYGSKPVEDSAITRTV
                     YAPWKWTHSTYTIETSRRLADFSLVEIDPSLRMADIDRKNNRLELKW"
     gene            19820..20503
                     /locus_tag="PLU11_01925"
     CDS             19820..20503
                     /locus_tag="PLU11_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPN57892.1"
                     /translation="MIKRKLIDGYPFVAYQPEEVSADEMKQRSLDFYTRLQERRSTRD
                     FSDRPVPREVIESVIRTAATAPSGAHKQPWTFCVVENQDLKKVIREAAEKEEYESYTG
                     RMPEEWLADLRPLQTDWQKPFLTTAPFLIVVFKKSYELKEDGRKGNVYYGSESCGLAC
                     GFLLAAIHQAGLAALTHTPSPMNFLSQLLKRPENEKPFLLIPVGYAAEDCWVPDLQRK
                     ALEEVSVWY"
     gene            complement(20500..21165)
                     /gene="rsmI"
                     /locus_tag="PLU11_01930"
     CDS             complement(20500..21165)
                     /gene="rsmI"
                     /locus_tag="PLU11_01930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPN57893.1"
                     /translation="MLYLVPTPIGNLKDITLRALEVLKEVDLILAEDTRTSSHLLNHY
                     QISKPVTPYHQHNEHKVVDHLVSQLLEGKKMAIITDAGTPGISDPAFLLVRACIKAGV
                     KVESLPGATAFVPALVNSGIPSNRFCFEGFLPLKKGRQTLMKQLAEEERTMIFYESPM
                     RLVKTLEEMATYFGPERLCSVSRELTKMFEENKRGTLQEVAAYFKEKTVKGEIVLIVA
                     GKS"
     gene            complement(21251..21496)
                     /gene="purS"
                     /locus_tag="PLU11_01935"
     CDS             complement(21251..21496)
                     /gene="purS"
                     /locus_tag="PLU11_01935"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPN57894.1"
                     /translation="MTYTVQIKVMPLKELLDPQGKAVMGGLANLGLNGVDDVRVGKHI
                     TLQVNADSPENAKAIADEASKKLLSNAVMEYFEIVVD"
     gene            complement(21493..>22206)
                     /locus_tag="PLU11_01940"
     CDS             complement(21493..>22206)
                     /locus_tag="PLU11_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPN57895.1"
                     /translation="LAIVAVLQNGIIIHVTAEGSELLDIPEKIWLASLFIGIAAVVDF
                     LDGFVARLFKATSEMGKQLDSLADVVSFGVAPGMIIYQFLRLSFAKDPSGVDTSLLWL
                     VPAFILPCAAAWRLARFNLDTTQTYHFKGMPVPAVGLLVASLPMIYFYSTDQWMVDLL
                     LNKWFLYGVVAVLSWLMVSTLPLMAMKFKDYSVKNNLPKYLLVGVAVIAALLLKWLAV
                     PVIILAYVVLSLLFKNKIA"
BASE COUNT         5986 a         5225 c         5064 g         5821 t
ORIGIN      
        1 gtgtataccg atatggatcc ggctcctgtg gccagtttag tatagtatga agaaagtatg
       61 ttgttttggt gaactcttat tgcgtctccc gccggctaac ggcggggaat ggctgcgtca
      121 taaccagttg cccgtatttg tgggcggcgc ggaactgaat gtggccactg cattagccac
      181 ctggcagtta cccgtaaagt actgcacggc tttaccggac aatattattt caaaagatat
      241 ccgggattac ctggaacaaa aacatattga cacgtcttct atcattctct ccggggagcg
      301 catcggcctt tactatttaa aagaaggggc agatatgaaa agtcatgaga atgtttttga
      361 ccggaagtat tcttcttttt ctacactggg aaccggcatc gtggactggg aaaccatctt
      421 cagtgatgta agctggtttc atttcagtgc aattgcacct gcggtgagtg aatcagcggc
      481 agcgctttgc aaagaagcat tggaggaagc aaagaaaaga gggatcacta tttctgtgga
      541 cctgaatttc cgttccctgt tatggaagta cgggaagcaa ccggtggaga tcatgcctga
      601 actggtgcaa tactgtgacc tcgtcatggg aaatatctgg gcggccaata cactgttggg
      661 gattccgttg aacgaggagc tgattgcggc gaaatcatat accgcccatg cccgttttac
      721 tgcatcagaa atctttaccc gttttccgca gtgcaactgg atggccaata ccttccgttt
      781 tgacggcaat gcggaggaga tcaggtattt cgccacactg gataaccgga cagaacaagt
      841 agtatctcct ttattacaga ccgattcggt agtggataaa gtaggcagcg gggattgttt
      901 tatggccgga ctgatctatg gcctgatctc cggaaacccg gcagatgaga tcatcagttt
      961 tgcggcagcc gcggcttttg ccaaactgca ggaaaaagga gatgccacac aaaacagcgt
     1021 ggaaaagata aaagcgtatt tacaaaaccc tgtatttaat taatcagaca atattcaatc
     1081 aacacaaaaa ttcgacgaca tgagtaagaa agtagtaacc ctcggtgaga tcatgctgcg
     1141 tttatccacc cccgatttca aacgttttgt acaggccgat acctttgaca tcacgtatgg
     1201 aggtggcgaa gccaatgtgg cagcagccct gtgtaactat ggcctgaatg gcacgtttgt
     1261 aaccaaagtt cctaataacc cgatcggtca gagtgccatc aaccacctgc gtcgttatgg
     1321 ggtagataca caatttgtag cacgcggtgg tgaacgtctt ggcatttatt tcctggagac
     1381 aggcgcttcc atgcgcgctt cccaggtagt gtatgaccgg gcaggtgctt ctatcgccga
     1441 tgtaaatctg gatgaatttg actgggataa aatttttgac ggcgcggatt ggttccatac
     1501 cactggtatc accccggcgc tgagtgataa agcggctgcc ttagccgaag ccgccctgaa
     1561 agccgccaaa gccaaaggga tcaccaccag tattgacctt aactaccgga agaaactctg
     1621 gagcaaggaa aaagcccgtg aagtgatgac ccggctctgt tattttgtag atgtatgtat
     1681 cggcaacgaa gaagatgccg acactacttt aggattcaaa gccggcgaaa cggatgtaac
     1741 aaagggtgaa ttgcacctgg atggttataa agatgtattc cagcagatga aggccaagtt
     1801 cggtttcaag ttcatcgcct cctcactccg cgaaagtcat agtgccagtg ataacggatg
     1861 gagtgccctg gtatatgacg gtactgactt ctaccatacc aaaaaataca atgtacggat
     1921 cgttgaccgc gtaggcagtg gcgacagttt tgccagcggc ctgatctatg gtctcgttac
     1981 cggtatgtcc atgcctgatg ccgctgaatt tggcgtagcc gcttccgcac tcaagcannn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntag caaacgcccc caaatacttc
     2161 tccgttcacc cgttgtttga aaaggccttc gcctttatca gcagcaccga tctggccaat
     2221 gctccggacg gaaagtcgga gatcagtgaa ggactaaaag cgatcttcag taatgcacaa
     2281 ggaaaaacgg ctgaggccag cctgtccaag tttgaatgtc acaataagaa tatcgacatc
     2341 cagctttgca tcagcggtgt ggagaccatc ggctggacac cccgggaaaa atgtgtaacg
     2401 cctaatggag gttacaatga tgaaaaagac gtgcagttat tccacgacaa ggccgacacg
     2461 ttcttccagc tcaccgatgg gcagtttgcc attttctttc cggaagatgt gcatgcgccg
     2521 atgatcagtg agggagtggt gaagaagttg gttattaagg tgaagatttg attcagttgg
     2581 cagtaggcag ttggcagttg gcagtaggca gtcggcagta ggcagtcggc agtaggcagt
     2641 tggcagttgg cagtcggcac ctaagaaaca atttcaaatt accggagaaa ccttttcata
     2701 agaactattc gtttttataa gttaagactg gaatagtttt taagcatctt cgaactatag
     2761 gattttttac tttttaattt tgacttttta attatactat tatgagtaat actaagaaaa
     2821 taaccgacgc catcatcgcc cagggcatcc tccccctcta tttcaatccc agtgaggaaa
     2881 taagcctgga tacattgaaa gcgatttaca gggcgggaat caaagccgtt gagtatacaa
     2941 accgcgggga agcggcgctg agtaatttta agaagatggt ggaactgcgg aatgcagaga
     3001 tgccgggctt gttgctgggg gtgggcacca ttaagaatat gggacatgcc actgattata
     3061 tggcagcggg agccgacttc ctggtgagtc cgggatttgt accggatgta gccgcatact
     3121 gtgtggctaa tgatatattt tatgcgccgg gttgtatgac ccctacagag atcattgccg
     3181 cagagaatgc cggcattggt tttatcaaat tattcccggg aaatatgctg ggaacggagt
     3241 ttttaaccac aattaaagat atcttcccta aactgttgtt tatgcccacc ggcggcgtgg
     3301 ataccacgaa ggaaagtatt gaaagctggt ataaagccgg ggtttgtgcg gtgggtatgg
     3361 gcagcaaact gatcagtaag aaactgatgg aagcaaagga ctatgcgacc atggaaggcg
     3421 aaacgaagaa agtactggaa ctgatcagca gtatcaaacc ttaaaattac acacatgcag
     3481 ggccaaatga aaagtaaaat gtcgaactac agatgggtga tcgtttccat gctgttcttt
     3541 gccactacca ttaattatct cgaccggcag gtactttccc taacctggaa agactttatc
     3601 gttcctgaat ttcactggaa taacaatgac tatggtacca tcaccgcgat cttctcattg
     3661 gcgtatgcat tctccatgct gctggccgga aaatttgtag attggatgga taccaagaaa
     3721 ggttttctct gggcaattgg agtctggtct atcggtgcct gcctgcacgc ctattgtgga
     3781 atcgctacct ctggtatcct gactggtacc tggttggtgg gttttgaagg agccaaggac
     3841 accattgcaa ccgttagtga tgtggccaaa atannnnnnn nnnaataact tcactcagtg
     3901 tcacactgtt catctttgcc cgtattgttt tggcactggg agaagctggt aactttccgg
     3961 ccgccatcaa agccactgca gaatactttc cgaaaaaagc aagagggttt gccaccagca
     4021 tctttaatgc cggcgccact attggtgctt taatcgcccc gatctccatc ccttttatcg
     4081 caaaagccta cggctgggaa atgtctttta tcatcattgg tgcgttgggt tttgtttgga
     4141 tgggtttctg ggtattcatt tataagaagc cggaaaaaca ccctaaagtc aacagcgaag
     4201 aactggccta tatccagcag gatgacaaac atgcaaatgc ggaagaaatg ctggcccaca
     4261 ctaagaaaat atcctttgcc aaagcattca gctataaaca aacatgggcc tttgccattg
     4321 gcaaatttat gaccgatggt gtctggtggt tctatctgtt ctggacaccc gcctacctga
     4381 gttcggtgta ccatatggat tcaacgcaga gtgctccgca gatcttcacc ttgtatttta
     4441 tcactctact gtcaataatc ggtggctggt taccaaccta ttttgtagag aaaaaaggga
     4501 tgaaccctta tgccggacga atgaaagcga tgctgatctt tgctttcttc ccattattag
     4561 cacttttcgc ccaaccactc ggttacttgt ctgtttggta cccggtcatt attataggta
     4621 ttgcaggcgc agcgcaccag gcctggtccg ccaatatctt ctccaccatc ggagatatgt
     4681 tcccccggtc agctattgct accattactg gtattggcgg tatggcaggt ggaatcggct
     4741 cattcctgat caacaaaggt tccggaaaac tgtttgatca tgcggcgaac accaatatga
     4801 aactgatggg ttttgaagga attgaagccg gctatttcat catctttacc atctgtgcgg
     4861 tagcctacct gatcggatgg gtaatcatga aatcattggt gccaaaatac aagccaatca
     4921 ctgatttata aaaagagctt attcattttt aaaaatacgg cacccgcatt ttggcagtac
     4981 cgtatttttt ttcacttatt attgcccccc ctctcaataa ctgcttcaga acgtgacatc
     5041 cctggataat gttctattaa aaatcgtagc ctttacaaca atcggcagca gtgaaaaatg
     5101 aaaacaaaat gtattgagga aactgtctga aagaaagccg gatctttcgt atcttgaaaa
     5161 tggagcctag tttctgcatg tctatccgat tgacctggta ggctctggac cagttttgta
     5221 caaatccagt aagcagaata attaattcat tcaccagtat gaaatttcag acccgcctga
     5281 ttaattttct cctgccgttt gcggcttacc ttctctttgc ttttcagtgg ttcaatactt
     5341 atgttaaagc ctggtatgcc aatataccag aagaaacaca atatctgagg ggtgcactta
     5401 ccaaccccta tatttattat acctggctgt ttggcataac ttcccttcta catctcacaa
     5461 ttggtatcgt ctacctggtt cgtgaaaaaa ctacccgccc gttgatttat ttcctgatgc
     5521 tcaacctgct gtttaccagt tactatgttt ttcttttcgc tgccgatttt cctaaatgga
     5581 gcagaaccgg tccgttgaat tttgtgattt tgtgcttgct tggaatatcg attgtcaggt
     5641 attattttac actttaccgg agagaagatc gtgaataaca aagaaaatag tcaattggac
     5701 tattttctct caatgtgaaa ttgaattcat gcggattatc tatatagcga ttttgaaaaa
     5761 aatcatgtgc taagatttat aaacagttgc aatcaaaaag gaataaaatc atgataacaa
     5821 aaatagtctt atcgctgatc acggcctttc tgtttattac tggtgtattt ttaattatta
     5881 cgaataaaga aatcgagggc aatttttacg acagtactgc cggcagaggc tgggtacaca
     5941 attccattaa tggctatttt cttgtactta tatcaattgg ctttttaata gcactcattt
     6001 ttgtaataaa agggaaaaaa ataaaaccat agtcctgtcg agttatagaa ccggtttttg
     6061 ggcgcaaata aacagcatgc tgctgtttgc caaaaaacat ttagcaaata gaactacttt
     6121 aaaaatatgc tattaaggaa atgacggtca ctttaacaag cctcattgcc tgcttttttg
     6181 ccgctttaaa aaatattgat tatgaaattg ccgtctatac tattcctctt gttcctgctc
     6241 aaattaacca gtgaagcgca agacagcctt cgcaaagtga atattcttgc cccggattca
     6301 aaaacagtat ttattggaga actgggtcaa tcgttaggca gctttcttac tgttcaagga
     6361 gttgtagtgg atggaccaga caaaggatat gaagaaggcc ccaacctgat cgtacatatg
     6421 ataaacgaca gtgccaccca ggtaccccta caaatacccc tgtcccccta ttttggctca
     6481 tttggcaaag ggacaatgcc cgtcatcaaa acaggtaata gttatcgttt acgggtatac
     6541 gagaccggcg aatttattgg cagtcccgac ggggctttca acgaagccgg ggtggtcata
     6601 caaacaaccg gattctattt taaaaaccgg ctgatcgtac tcgctggcga aaaaacggca
     6661 ccgcttgaat ggcaccctgc tcaatttgaa ggtcgtgagg ccttactcac gggcatagcg
     6721 gaaaatgaaa atgataccgc ctgtattcgg ggagccagtt gggtactgaa actggtaaat
     6781 gttccgaaat ggactcccgc cgaaatcgga aaacaggcag aagtatacgg gaagatacag
     6841 ggaacaccgg caaaaaatca gttttacgta ttacagggaa gatccaggct aatacgactg
     6901 gaagatcagc tgggaaaaca ggtcagtctg cgtggattgg ccatcagcca aaaccaatac
     6961 tggtggttca attaccgggg taccgatatc tatgtggaaa aaatggaaca gctgccaggc
     7021 tggtctgatg aaaaccattt catggcaatg gaaataacag ggatactgga tcaggaaaaa
     7081 cttccgcgac tggatcagat tgctgtgaaa gataaccggg atttaaaaat gtattatata
     7141 atcaggaatg caaaatggac accggttaac aaattactgg aacctgagtg gtttgaattc
     7201 agataagaag ttattctcaa catcagattt attttaagtc tggtataaaa gtgaatgata
     7261 ccaccaatga ataaagacac cctcgcctac aacaacgccc aaagcgtaac agaaaagcca
     7321 atctgtcaat tgctggcgtc aacaatcaat gccgccctcc ccgaagcgga aaacaaaatc
     7381 tggcatgcga tccccgtctg gtttctcgat ggcaatccag tggtggggta tagcaaactg
     7441 aaaaatggta tccggttgct cttctggagt gggcagacat ttgaagaagc tggtttaaaa
     7501 aacgaaggca agttcaaggc agcagaaatc cgttatacag cccttgacca ggtgaatacc
     7561 aaagaccttc aacgatggct gaaaaaagca cgtgatattc agtgggatta taaaaatatt
     7621 gtgaaaagaa aagggcaatt ggaacgactg taatgaggca aaagcaggct tttcaaaata
     7681 cgcttttata tttgtacttt taaaggactc attttatcac ttccgttgtt ttacaaattc
     7741 gcaggaccat gagaatatgc agcgcactcc tccttctgat ttttctgact ggctgcttta
     7801 aaagaaacga gtcaaatgta aatgcgcctt gtacagacag ttgcatggtc tttaatatcc
     7861 gggtaactac aggtctccat tcaaccacca ccatggccaa cattccgctt gaactgggct
     7921 ggtcaaaacc ggccactccg ctgggtgatc ccgggcgatt gatcgccaaa ggaaaaaccc
     7981 agggcaacgg aacaatcagt ttttcgttta aggctaaagc gaaagaatta caaggtggaa
     8041 aattctatat taccgcctta gcgggaaacg attatttccg gactcaaaac gagtattatg
     8101 gaatcgggaa atatgacagc ctggtaaata cagctattca tgttccagcc aaagcaacat
     8161 tgaaaatcat ctataaaaac ttcaacccgg tcagtacaga agattatttc caatgtcttc
     8221 cctattttct gtcttatggt tcaacagggc atcccataga aatgaaaaag ccggacggcc
     8281 aactcagcaa tactcacttc tttacatcat acgggccgtt tacaacactg gagcttaccg
     8341 gtactacagc tggcgaccag tatacgtacc ttgatattat taaaaagaaa aacggagtac
     8401 gggtggatat aagagacagt atttatattg taaaaggaga gactaagaca tacgaagtaa
     8461 gtttctgaga caggtgaatc tcatccaatt gaagtagcgc ctgcaaaaca cgattcattt
     8521 acctaacaac tacctgatat cttccaaaaa aaatgctcct caacaggagc attaatcttt
     8581 tacatcatat tgtgtgaaac cttaatcaca tccaagcttc ctcttcattc ttcgacatga
     8641 tcatctttcg gcaagctcat ccttcggcaa gctcaggatg acaaggacac ttccctactc
     8701 ccgactcccg actcccgact cctgactccc gactactaac tcctaactcc cgactatcct
     8761 actctcccca gatattatcc ttcccatcca gccattgttt caccaggtcg gtggtcactg
     8821 attttggtct ttcgaggggg aagccaaggg cacgatccca gcagagagaa gcaagtacac
     8881 ctaaggcacg gcttactgca aaaagtacgg tatagaattc atattccacc ataccgtagt
     8941 gtactaacag ggcgccacta tgcgcatcca cattcggcca ggggttcttg attttaccga
     9001 ttgtctggag aatcggaggt acggtttcgt atacattcca gatggtctgt accagtttat
     9061 catccggcat atgtttttta ccaaactcca tttgcgcggt gaaacgaggg tcagttttac
     9121 ggagaaccgc gtgaccatac ccgggcacca ctttcccttc gctcagggtt ttcttaacat
     9181 attcggcaat ctgttctttg gtgggcaggt cggtattcag ttcttcctgc atttcaaaca
     9241 cccatttgat cacttcctga ttcgcaagtc cgtgtaaagg accggccagt ccgttcatac
     9301 ccgctgcata agaaagataa ggatcactca gggctgaacc taccaggtgg gtggtgtggg
     9361 ctgatacatt accgccttca tggtcggcat ggatggtcat gtataaccgc atcagttcac
     9421 ggaaactttc atcatcataa ccaagcatat gggccaggtt acccgcccag tcgagcagac
     9481 cgttgggttg aatatgatcg ctgtttttat atttacgacg gtagatgtaa gctgcaatac
     9541 gtggcagacg ggccagcagt acgatactgt catcgaatac cggcaaccag taatcttttt
     9601 tactgatccc ttccccgtat tttttctgga aataactttc tgtctgcaag gccatcacgc
     9661 ctacgacaaa catcgtcatc gggtgtgccg tgatcggcag ggcatcaatc gcttcaaaca
     9721 cgtgggtagg tacgtgactc cggcgttgaa gaatattggt cacatgttct gcatcttcat
     9781 cggtagggag ctccccgatc agcatcaggt aaaaaaggcc ctcaggtaaa ggttcagacc
     9841 ctccgggagc tttgggcagt ttggcctgca gttcaggaat agaatgaccc cggaaccgga
     9901 ttccttcctg ggcatccagg agtgaagtct ctgtcaccaa accggtaatc ccccgcattc
     9961 cctggtaaat ctgggagagg gtgacttctc caattacttt attaccatgt tctttcagta
    10021 agtcctttat ttcggcagct acgctatccg ctttagctct gaaccggtct tttattattc
    10081 ccataacacc ttatttttcg gggatttttg aaaaaaatta gaaggcataa aattactgaa
    10141 tgcccggggt ggtaacaaat aataaaaggg aaagtttgct aaagtagtaa gtacgaagta
    10201 cgaggtacga ggtacgaaag gcaggccgta aaaccaggaa tagtaggata tttaagatac
    10261 aatcttctta gaacgggctg tatactcccg actcccgact acgaactccc gactctactt
    10321 tggcgttttc cgatacaacc acaccgccac gaacaaaaac acaatattag gcagccaggc
    10381 ggctaaaaag gggtgaaaat tgcccttggt ggcaaatacg gtggagaacc ggtctgaaat
    10441 gataaaaaca gcggcaataa caaagccaat agccaggtgc attccactgc ccccacggat
    10501 ctttcggctg gcgatcacgg ccccgatcat ggtcagcaat aatacggtag ccgagaccgc
    10561 tgtgcgacgg taccattcca cctgcagggt tttgagtcct tccgtgcccc gcatttcctc
    10621 ccgcttaatg taataagcga gctccggaga agtgagttta tccttgaggt aatcatcttt
    10681 ccgcaattcc tctggtttga gattcatgct gagaatcatg gtatcccggt ttaccagccg
    10741 ctcgcccaca ctgtcaagat gccgttctcc aacccggaaa agcttccatt ttttagtgag
    10801 cgtatcccag gaaattgact ctgcacgcag gttgtacacc actttattct cttttacgcg
    10861 ctccaggaaa aatccacggg caaactttgt agtggtatca taatccctga taccgataaa
    10921 cgtattggta tcaatccgca ggtaatagca acgggcacaa ctgctgaagg ttcgaccctt
    10981 tgaggggtct ttgctgtcga tatactttga ctgaaaattg gctttaattg tattcgcttt
    11041 cgggatccag taactgttgc ccacccagag aaccgcagcc aggaaaaacc ctccgataaa
    11101 ataaggacgc agaaaccggt tatagctggt tccgctggcc aggatcgcaa tgatctcaga
    11161 acgggtggcc atccggctgg tgaaaaaaat tacggcgata aacacaaaca acgggaacaa
    11221 caggctccag atccagggca caaacccgag atagtattta ttaatgattt ctttggtact
    11281 gaatccggct gttacaaaat catccgcctt ctcgctgctg tccacggcca cggcgatgac
    11341 cgtgaataac agcatgcaga agataaaggt gaccaggaat ttcttcagta tgtaccagtc
    11401 gagtttgtgc atggaatggc gaaaataacc aattaactgt tgacgctttg tgaaagcctg
    11461 acttataact tttgcttgag gcgggggatg atttccgctt tccaggccgc aaaatcgccc
    11521 tgttggatat gtttccgcgc ctcccctacc agccaaaggt aaaaagcgag attatgcaca
    11581 ctggcgatgg ttaatcccag tatttctttg gccttcatca ggtgccgcag gtaggatttg
    11641 ctgtaatacg cactgatttc gcaatcgata ctttcatcaa taggcgaaaa atcaaattcc
    11701 catttcttat tgtcaatatt gatcactcca ttggacgtaa agagcatggc attgcggcca
    11761 ttgcgggtgg gcatcacaca gtcaaacata tccaccccca tgctgataca ttccaggata
    11821 ttccagggcg tgcctactcc catgaggtag cggggtttat cctgcggcag gtgatcacag
    11881 caccagtcac aaatctcata catcaccggt tccggttcgc ccacactcag accgccgatc
    11941 gcatttccat tggcctcttt tgaagcgata tactcacagg attgtttgcg caggtcttta
    12001 aatgtgccac cctgcacaat gggaaaaagg ttttgtgtat acccatactt cggctccgtt
    12061 tcattaaacc gggtgaaaca acggtccagc cagcgatggg tcagttccat tgatttttta
    12121 gcataggcaa attcactgcc acccggcgga cattcatcaa aagccataat gatatccccg
    12181 ccgatactgc gctggatatc catcacgttt tcaggagcga ataaatgctt gctgccatcg
    12241 atatgcgact ggaaagtcac cccttcttct ttgattttcc gggtaccggc cagtgaaaaa
    12301 acctggtagc ccccgctatc ggtcaggatc ggtctttccc agccattgaa ccggtgcagg
    12361 ccacccgctt tttccagtac atcgagtccg ggacgcaagt ataaatgata ggtattcccc
    12421 aggataatct gggctttcac ctcattcacc agttgttgct gggtcacggc tttcacactg
    12481 cccaccgttc caacgggcat aaatatgggt gtctgtattt ccccatggtc ggtagtgatt
    12541 ttcccggccc gggccttgct gtcagtatcc cggtgactca gttcaaaatg aagtgctgcc
    12601 atagggctgc aagataaatg gaaatgggct gtttccgggg ctgaaatgga aaattgaaga
    12661 ctgacgccgt aagcggaaca gcggcttatt caatttcttt ttcaacatat acaatacttc
    12721 catcagccgt aataccttct gcaatgatcc tgattttttt ggtgcggtca ctgttataga
    12781 attgaatctc tgtagtcccc ttttctaaag gcgtgttcgg tttccagctg atcgttttgc
    12841 gcagatcctt ttccactttt atttcttctt tccggtaatc cggctctctg aactccggtg
    12901 aaagcgggta acctgtcagt ttttgttcta atatctggaa cctgtctttt tcccaataac
    12961 cctcatcacc cggttttttc agaaaaatgg ccagtgcggg gggcagtgtt ttctcgattg
    13021 acaggttctg tacaaacttg atcgcagcta tctgatccag tgaaataaaa tccagctgat
    13081 tcctgtccac aatggaattg tcaacataaa actggtccac catcatttcg cccgttagtc
    13141 cacgaccaaa ataaataagc tgacgggcgc cgtttacgaa ccgtacatcc acgcgcttca
    13201 ggttggcacg catataacta aagatatcca tgtagcgcat ccttccggga tcattcaata
    13261 catccagtgt ttcgcttgtg gcagaatatc cgctgacaac actaccagca atatattttt
    13321 cctcgagcgc ctttaatctt ttggtaacct gaacactgct gttcgccgac agtaccactt
    13381 cggagagtgt aatggatttt tcagtaaagg ccaatgtatc gacagcatcc gtcagtgagg
    13441 atgatggcga tgctccccct gctgaataag cagcagctgt tcttcgtacc agttgttcaa
    13501 acgctgctaa caagtctgcc ggctgattgc tggacggtat attcaaccgg aaagtacaag
    13561 tgagttcctg tagttcatta cccggaatcc ggcaatgcac ataaacatcc ccatacaaaa
    13621 cgagatcatc aatgacaaat tttcccttgt tatctgttgt tactttatac aattcttttc
    13681 cattggaggc agaacgtata cccaccagca gattcttctc tgccaggggt ttcttacctt
    13741 ttataacggt cccctccagt tgaagataat tgccctttgg cagaacagtt gctgccggca
    13801 ctttgtcccg caccggctgt tgagaaaaag caacctgtac ggcaagatcc atttgttcag
    13861 gcgtactgat ggcccgaaaa gcacgaggaa ccggctgtcc atcgggtgaa agcagcgtag
    13921 ccgcaagacc ggatccaccc ttgttctcag cataaccggc atcgattaaa cttaccgaaa
    13981 gtacacggat cgttgtatct gcgaactgca acttcaccct gttctcacca cggggggaga
    14041 ggtctttttt aaccagctcc agcttcggtt gccccgtcag actgtgccgg ttaataaaga
    14101 caggtcgtgt ggccaatacg gtattatctg ttgccaacac ccggagttcg gccataccgg
    14161 aacggtaatc ctgcagggga atgaaataac tgaactttga cttctttgct aaattcatct
    14221 ccaccacata agccggttca tttccgatca tgaggagcat tttcaattga tgggccaaca
    14281 gatcaggact accaccatta tcaagatcaa aaaacatagt atccgggctt tgccggatat
    14341 ggagggctac accgatatca acggcatctg gcagcggagt tgagccagta cgacctcccc
    14401 cttcccaata acaggtgtag gtctcccctt ttaccggtgt aaatgtgaaa cggcccatcc
    14461 cgttgaagac tggattcaac gtggtcagcg tttctccctt gctgttacga actgatccac
    14521 gaaattcact cccggcctta aacaccacat ggttgagcgc ctgattgacc agggatcccc
    14581 cttccggaaa aaaaaccggc cccccgtcgc cattcagctc tttggcaacc gggacatcca
    14641 tcgctgaccg gtagagtgtc tttacaaact gatatggctg tgtttgacgg gccgtgacat
    14701 ctgaataggc ccggataaaa aatggagtgg atttcagccc ccggggaata gtgacagcac
    14761 ctgcacccac tccatctatc aggggaaggg tgatggacga ccattgccgc agcgaatcat
    14821 cccacaactc cacaaacaaa ttacggctgc tgacagaatc gatagcagac aggacaaacc
    14881 cattgaaaga gatcgtttct cctggtgcat aatagtcccg gtcagtttgt acaaagatcc
    14941 gttcagctga tccctgtgaa aaaacatgat tatggatgaa tagtaaacaa ataaggggga
    15001 atactgcttg ctttttcata cagttagaag gttgtataag gtttaagtgc tgtattgcca
    15061 gaagccggtt ccggctatct gcacattaaa tttactgcct taaaatacaa aaaaattaca
    15121 accatctgtt aaatcagcgc ttatgcaagc cctacttatc ctactgctgt ggaaagcttt
    15181 tgccaactga atgttggcat tagcccggca cctctttacc ttcgccgcta tgccaccaat
    15241 tgcctgggaa gaaattctct tttatttctt tattgccctg acccttatcc agctgtttta
    15301 ttatggccgg tttttcagcc gggttgcttt ttttaaaccc aaagaacaac agcaatccag
    15361 gcagcatccg gtttccgtga tcatttgtgc ccgtgatgag gatgaaaacc tggcccgaaa
    15421 cctgcccggg gtattggtcc agcaatacag cagcagccat gaactggtgg tggtgaatga
    15481 caactccctg gacgactcca agtatattct gcaggaactt aaaaaaacat tcaaatccct
    15541 tcatgtggtc gaactgaccc aggaagccaa gctgatttcc ggaaaaaaat atccgttgtc
    15601 catcggtatc cgtgaagcaa aatatgaagt gttgctgctg accgatgcgg attgtgtacc
    15661 cgcgagtgaa cattggattc agaaaatgca ggaagcatat accgggggca ttgaaattgt
    15721 attaggctac ggagcttatc atcgccgccg gggtttgctt aacaagctga tccgttttga
    15781 aacttttcat acggcactgc aatacctttc gtatgcctta tcgggtattc cgtatatggg
    15841 agtgggccgt aatctttcgt ataagaaaga cctgtttctg cgcaacaagg ggttcgcttc
    15901 catcaatcat attccgagtg gcgatgatga cctgttcatc aataaagtcg ccaccaaaga
    15961 gaatactgcg gtggtgatcg atccggatgc cattacccgt tccataccca aaaccacctg
    16021 gggcggctgg ttaaaacaaa aatcaaggca ttataccacg gccaagtttt ataaacccaa
    16081 acataaattc ctgcttggtt tgtattttgg ttcccagttt ctttattacc cggccttcgc
    16141 tgcggcagcc attttctttg actggcgttt tgtattaccg gttttcggcc tgcgccttat
    16201 cctgcaggga attgtcttat ataaatccat gaagaaaatg ggtgaggccg atctctggcc
    16261 ctggtttatt ttccttgata tgtggatgtt cttttattat ctcatctttg caccggcttt
    16321 gtggagaaga ccccgacgga gctggaactg acctgaatga ttatgcaaca catcgacatc
    16381 ctctataaat attttacgga attcaccccg cagcagctgg aacaatggca gctgctggaa
    16441 gggttgtata aagaatggaa tgaaaagatc aatgtgattt cccggaagga tatcgacggt
    16501 ttatatgaaa aacatatcct gcattcactc agtattgcgg cttcttttga atttgaaccc
    16561 ggtatggaga ttattgatat tggtaccggg ggcgggtttc ccggtatccc gctggccatt
    16621 tttttcccgg aagtgaagtt tttactggca gacagtattg ccaaaaaact gaaagtggtg
    16681 gaggccgtgg ctgcaggaat cgggctgacc aatatcagta cccgccatac ccgtgcagaa
    16741 gaaataaaga accagaaatt tgattttgcc gtttcccggg cggtggcacc gttaaaagag
    16801 ctatggtcct gggcccgccc cttgcttaaa accaagccga tgtcaccgga tgctcaacat
    16861 aaacccggtt taatttgtct gaaaggcggc gatctggcaa aagaaataca ggaaagtagc
    16921 acccggccac atatgattga aataaaggaa ttgttccccg aagaattttt ccaggaaaaa
    16981 tacctgctct acgtaccccg gtaaaatata ccacttagtg gtataggagc ctattgatta
    17041 attgcagaac tttaaagaat gaggcagatt acggttttta ttgtagatga taataaagag
    17101 ctcagaaatg ccctcgaaga gatcgtttcg atgtcggaag gataccactg tattggtacg
    17161 gccagttctg cggaagaagc cgtagaccgt attcccatta tccgtccgga tgtggtacta
    17221 atggatatca acctggggac ggaagaaagt ggtattgatg tggttcgtac cctgaaaccc
    17281 cggatacccg ataccaattt catgatctgt accgtgtatg aagaagacga gaaaatattt
    17341 gaggccctca gtgccggggc aagcgggtat atcctgaaaa aaacaccgcc gggccggatg
    17401 ctcgaagcca tccgggaact ctatgagggg ggtgccccca tgagcagcca gattgcccgt
    17461 aaagtagtgg ctgcttttca gcatcagcaa agccagcata gttttaccga tccggaaaag
    17521 ctgaatgtac tgaccccccg tgaaatggaa atacttgaac tcctctccaa aggactgatg
    17581 tataaagaaa ttgccacaca aacctttctg agtccggaaa cggtcagaaa gcacgtttat
    17641 catatctacg aaaaactaca cgtaaccaac cgcgtagcag ccgtcaataa gtactacggg
    17701 aggtaagatt tgcaggtttt tgcgggttat tttatcaaaa agggcaaaaa ataccacaaa
    17761 agtggtattt gagggtatct gcagtcagcc tacttttggg taagtaaaaa gcattacaag
    17821 aacccttaac cttaaactta tcctgtcacc cgatataggg ccttgattca aaccattgac
    17881 aaagcccctg ctttccagca ggggctttac tttttaggtg aatcagttgt tttacattac
    17941 catttcagtt ccagtctgtt gttcttccgg tcaatatccg ccatccggag agagggatca
    18001 atctcgacca ggctgaaatc agccaagcgc cggctggttt caatcgtata ggtgctgtgc
    18061 gtccatttcc agggtgcata taccgtacgg gtaatagctg aatcctccac cggtttactg
    18121 ccatacatca ggttaagcgg gatataatgc agttccttac tgccatcttt aaaactgatc
    18181 tggagatcaa tgggcatagg catcagaccg gcccggttca gcctcacttt ggtcttgccc
    18241 ccttcttccc acaagctatc aatactgtaa tcaatggtct tggtggaatt gacccaatac
    18301 tctttgtacc aatccagttt catatcactg actttctccg caatccggat aaagtcgctg
    18361 gcattgggat gtttgaattt ccacttatta taatattcaa tcaggatccg gtctctgacc
    18421 gacgccccgg ttatataccc cagttgttcc aggaatacgg cccccttgga ataagcagct
    18481 gctccatagg cgctattggt attaaaatga tcagcgtgcg tagtcagggg ttcttccttg
    18541 ccgctttttg ccagcgactg atagccgcgg tagttcccat caaaggcaaa tcccttgcta
    18601 ttatcgagat aggcggttac caggtcttcc gcgtaggatg taaagccttc gtccatccag
    18661 ccatagagcg attcattggt gccgaggatt ccatagtacc agttgtgcag ccattcatgc
    18721 aaccacgcac ccggaccgat cagcaaagtg gccatcgggt attccatccc cccatcaccc
    18781 ccatgcacaa acgaaaattg cctgtacgga taggtgccga attttttctc cataaaggga
    18841 agcgcccgtt gcgcatcatc cagtatctgt tcccaggctg ccgcctgtac attgctgact
    18901 ttatagagga ggtgaaatgt caggctgtcc ctggctttta ctgatttatg aataaaatcc
    18961 ggatccgccg cccacatgaa atcatgcaca ttgggtgcga ggaatcgcca ggtcagttta
    19021 tccccggcag gccgtaccac tttggcaccc ggtgcttcat atccataccc gatctggttg
    19081 ggattctgca ggtaaccggt tccgcccagg atatattttt tatcaatcga gatacttaca
    19141 tcataatcgc cccaaactcc ataaaactcc cggcccacat acggggtggg atgccagcct
    19201 tcataatcat attcgcaaag cttgggatac cactggctca tggagaacct cactttactg
    19261 gagggattat cccggccact gcgccggatc tgcaggggca cctgggcctt gaactccata
    19321 tcaaaaacaa ctttggatct gggcaggatg ggtttatcca atctgacttc caggatggtc
    19381 tcatccatca ggaattgttg cacccttcca ttcattttaa gcgacagaat ttcctggtac
    19441 ccgatttcat caagtttgag cttctggatg cggtccttta cccgcatatc ccagtctgcc
    19501 ccgcgcccgg ctgtgatcgt tccctgccgg cggctgcggg tatccatcat actgccgggc
    19561 tggaaagcgt tgaagtacaa atgatagaaa acccgggtca aggtatccgg agaattattc
    19621 cagtactcga gtttctgttt acctgtaaac tgattggtct gcacattcat gtcaatattc
    19681 atgacatatt ttactttctg ctgccagcgg tctggctggg caacggcggt ttgaagtgcg
    19741 agcaccgtaa tcagggagaa aaaaacctgt ttattcattt attctggttt gaattggtaa
    19801 atttcgccaa atacctgtta tgataaaaag aaaattgatt gacggttatc cttttgttgc
    19861 ttaccagccg gaggaggttt ctgcggatga aatgaaacag cgaagccttg atttctatac
    19921 ccgattgcag gagcgacgtt ctaccaggga tttttcggac cggccggtgc ccagggaggt
    19981 gatagaatcg gttatccgga cagccgccac cgcacccagc ggggcacata aacaaccctg
    20041 gaccttttgt gtggtggaaa accaggacct caaaaaagtc atccgggaag cggctgaaaa
    20101 agaagaatat gaaagttata ctggccgtat gccggaggaa tggctggcgg atctgcgccc
    20161 gttacagacg gactggcaaa aacctttttt aacaaccgcc cccttcctga ttgtggtttt
    20221 taaaaaaagt tacgaactaa aagaggatgg ccggaaagga aatgtgtatt atggcagtga
    20281 gagttgcgga ctggcctgtg gttttctgct ggcagccatt caccaggccg gattggcggc
    20341 ccttacccat acgccatcgc cgatgaattt tttaagtcaa ctgttgaaac ggccggagaa
    20401 tgaaaaaccc tttttgctga tcccggttgg atatgcggcg gaggattgct gggtaccgga
    20461 tctgcaacgt aaagcactgg aagaagtatc ggtctggtat taggatttac ccgcaacaat
    20521 aaggacaatt tctcccttta ctgttttttc tttgaaataa gcagcgactt cctgcaaagt
    20581 gcctctctta ttttcctcga acattttagt cagttcgcgg ctcacactgc acaaacgttc
    20641 cgggccaaaa taagtggcca tttcctccag ggtctttacc agtcgcattg gtgattcata
    20701 aaagatcatg gtccgctcct cttctgccag ttgtttcatc agggtttgcc ggcccttttt
    20761 caggggcagg aatccttcaa aacaaaaacg gttggaggga ataccgctgt tgaccagcgc
    20821 cggtacaaat gccgtggccc cgggcaatga ttccactttc accccggctt tgatacaggc
    20881 ccttaccaac agaaaggccg gatcggaaat accaggtgta ccggcatccg taataatcgc
    20941 catcttcttt ccttccagca actgactgac gagatggtcc accactttat gctcgttgtg
    21001 ttgatgatag ggcgtaaccg gtttactgat ctggtaatga ttcagcaggt gcgaactggt
    21061 ccgggtatct tccgccaata tgagatccac ctccttcagc acttcaagtg cacgaagcgt
    21121 gatgtctttg agattaccga tgggggtggg gacgaggtaa agcaaagttt ataggttgaa
    21181 aggttgaaag gttaacaggt ttaccggtaa gtattcttag aaggtccttg ttaacttttc
    21241 aacttgtaaa ctagtcaact actatctcga aatactccat caccgcatta ctcagcaatt
    21301 tcttactggc ttcatcagcg atggcttttg cgttctccgg agaatccgca ttcacctgga
    21361 gggtgatatg cttacctacc cgtacatcgt ccaccccgtt cagtccgaga ttggccagtc
    21421 cgcccattac ggccttccct tggggatcca gtaattcttt cagcggcatt acttttatct
    21481 gtacagtgta ggtcatgcta ttttattttt gaagagcaaa gataagacaa cataggccaa
    21541 aatgataacc ggaacggcca gccatttcaa cagcagtgct gctattactg caacccccac
    21601 cagcagatac ttgggaagat tgtttttaac cgaatagtcc ttaaacttca tggccatcag
    21661 gggcagggtg gataccatta accaggacaa tacagccacc accccataga ggaaccactt
    21721 gttcaataaa agatccacca tccattgatc tgtactgtag aaatagatca tgggcaagga
    21781 agccaccaac agtcccaccg ccggaaccgg cattccttta aaatgatagg tctgggtggt
    21841 atccaggtta aagcgggcca gtcgccaggc ggctgcacag ggaagaataa atgcaggaac
    21901 cagccagagc agactcgtat ccacaccgga aggatctttt gcaaaactca gccgcaggaa
    21961 ctggtaaatg atcatgccgg gggccacgcc aaagctcacc acatcggcca gtgaatccag
    22021 ttgtttgccc atttcagagg tggctttaaa gagcctggcc acaaaaccat ccagaaaatc
    22081 cactacggca gcaatcccga taaacaagga agccagccag atcttttccg gtatatccag
    22141 caattcactg ccttcggccg tgacatgaat gataatgccg ttttgtaata ctgctacaat
    22201 ggccag
//
LOCUS       DAOPMW010000018        21395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2308_length_21395_cov_10.263121, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000018 DAOPMW010000000
VERSION     DAOPMW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_21395_cov_10.263121"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1000
                     /locus_tag="PLU11_01945"
     CDS             104..1000
                     /locus_tag="PLU11_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57896.1"
                     /translation="MKNLCFFFLYLIIFPASAQVITPGQDTAAAVYYQQAGEYAVRNE
                     HAAAFRFLKEAIVNGARGEDVLTDTDFTGLQKDLNGWAEIEVLLKSQYVASNPGISKP
                     DLGYELWRMWVEDQRYRTLKRNYKLPGNPVTDIALHQQQLIRVKEIIKKSGWPAYSEV
                     GRAGGDAVFFVFQHDDAKNMKKVLPLLIAAAKAGEADMSKVAMMIDRYLAYTEQVQLY
                     GSQAFRRIKPGQDRKDIPLTLYPIAGEERMLERRKALGMVDFFENCRQLGVEYIRIEN
                     RTDYKPIVIKKKWVDAGYLLTE"
     gene            1402..2469
                     /locus_tag="PLU11_01950"
     CDS             1402..2469
                     /locus_tag="PLU11_01950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN57897.1"
                     /translation="MRSSQYTLFILLIVFASCKKDNSNPPAAETMYFPSNTDTTWERK
                     SMSSLGWNTSNVQALKDYLSQKNSKSFMILVNGRIVMEEYFNGHTASDTWQWNSAGKT
                     LVSTTTGIAQQEGLLNINTKASQYLGAGWTAAPLAKENLITVRHLLTMTSGLNDSNNL
                     VIRPNLTYMADAGTRWSYHNVFQKLIDVVAATSGQTFDNYFNAKLKNKLGMDGFWNYG
                     TIFTIYHSNTRSMARFGLMALNNGKWKDEQIINQTFFNEAINSSQSINPSYGYLWWLC
                     GKSGAMIPGGQTFYPGNLIPNAPTDAYAAMGAEDQRIYVVPSKKMVVIRMGDASDPLN
                     PSFAVSGFDNELWGKLNAVIN"
     gene            2582..3739
                     /gene="xseA"
                     /locus_tag="PLU11_01955"
     CDS             2582..3739
                     /gene="xseA"
                     /locus_tag="PLU11_01955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPN57898.1"
                     /translation="MNDSIKLSTLTQLIQDTIQSRFEGEVFQVSARIMNVKKYESARR
                     CYLTLEEYEYGSKITEIRAVFWSNAYSEIEKFEKALRQPFKDGAEITCKVKVRYHKVF
                     GLNLDVLQIDVAQALGTLELERQQTLEKLVKENPEYVQLIGGNYLTYNNRLELPLVIS
                     RIALITAPNSDGQRDFLQELKGNKHEYQFAVTEFLTTIQGENAHQLILDQLRLIEKQQ
                     ENFDMVAIVRGGGSISDFRPFDDYALSAAVARFPIPIFTGIGHDRNQSIVDLMAREQK
                     TPTKVAALIVEHNFDFENALIDLKARMSDAVTHQLDRAAEKLEQAKRIIRLSSPWDIL
                     RRGFAIITHEDKIVVDPSLIKEGMELQTQLMNETIHSTVTKKTKDENKTDI"
     gene            3717..3926
                     /gene="xseB"
                     /locus_tag="PLU11_01960"
     CDS             3717..3926
                     /gene="xseB"
                     /locus_tag="PLU11_01960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPN57899.1"
                     /translation="MKIKLTYSEAFNKLEELVEQLEDGTIPLDKLAATVKQANEWVAV
                     CEGKLRQIQEEVEADVVAKKSKGKK"
     gene            3993..4694
                     /locus_tag="PLU11_01965"
     CDS             3993..4694
                     /locus_tag="PLU11_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN57900.1"
                     /translation="MKYFLLHLLFLSALNSGAQHRNSFAFTIHVQPEITRHHKNYAIQ
                     WNEAGATTTFNAGLRAAVQYRIAGRFFAEAGLGYVPRRMNNTLYFFNQNVIPPPRQSP
                     TQELVGYKNITYRTLELPLTVGVIFLKLKKVELYATGGYTTNYLLSARYGKGSNSKYA
                     GVYSKRYWQGESLLAGIGTDIRIKEKLFFSTRLSYSLRNSVNADPYLFSQSYDGLRMT
                     HQFVQGAVGVRWSLN"
     gene            5099..6109
                     /locus_tag="PLU11_01970"
     CDS             5099..6109
                     /locus_tag="PLU11_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57901.1"
                     /translation="MAQKFLFCLLVFPALWVNSQSDFLDQLKIYKINDPVLKEIQFSV
                     FKKDMDKKKPLLLFLGGSGLEPTFKYNRADQQVYYTGFYRFSQYKDRYHIVFINKAGI
                     PLYDSVNNNNEQYKVTPFAEANNTADWRAQSASKAIDYLLKVLPVDTSNVIVVGHSQG
                     GQVAPKVAVLNRKVKKVVIMSANALDHIYDRMLLARQQALNNQMSYEEAQYVVDSLLT
                     IQKAIYQSPADTQQTFWGDPFNKWYSYSKETPLENMLKLNIPILLIAGGRDVEGSYIA
                     NTDYAALEFIRKGKQNLTYKVYPNYDHIYGETARKYGEEIGLGFKANAVVDDVFKWLG
                     EK"
     gene            6426..6974
                     /locus_tag="PLU11_01975"
     CDS             6426..6974
                     /locus_tag="PLU11_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57902.1"
                     /translation="MKILSAILLCFILFSCQQPTEKKSATPAVTSFDETAETAAILQV
                     IENETSCFFAGNYECWAKNWSHEPYAMQAWNNDDGSMDAAIGWEKINAQGKNWIEKYY
                     KNGKEVIHPVVKKEKPQVKFFNDRTAYLIWKQYNADKENKYYKLSQETRIMEKAADGW
                     KIVNVSAFWNSNKKIPVDSLKL"
     gene            7079..7489
                     /locus_tag="PLU11_01980"
     CDS             7079..7489
                     /locus_tag="PLU11_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57903.1"
                     /translation="MKRVFFLAGIIMLSCVVTAQSFKQAFFNADISNFWKAYDKIRAT
                     TDTALQRQLINDIYIRPGSAGLQELIRYRNYTDDDFLTMIRESPAFWKSLRSQTLNTE
                     KLYPVIEMDIQKLKTLYPPLKPATVYLLIGAFRT"
     gene            7556..8380
                     /locus_tag="PLU11_01985"
     CDS             7556..8380
                     /locus_tag="PLU11_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPN57904.1"
                     /translation="MADLPQRFHAYYRDFKPRENLALLCTHEYVHTQQNQPVDNLLSN
                     CLSEGIAEFISCKATGIASNTPAIAFGKLNTEKVVSQYVKDLFVMFNDYNWIWGENRN
                     EFRVRDLGYYIGYSIAERYYELSADKGKAIRELIELDYTNDAAVEHLVDTTRFLPKKL
                     SELYTDYDKSRPRVIALSPSINGTKDVAPGLTRITVHFSEPLNGINTGIDFGPLGPDY
                     FPKLSPVRVWSDDKRSITLEADLKPGKTYQFMIDNTFRREDQVRIKPFVVEFSTKE"
     gene            complement(8427..9359)
                     /locus_tag="PLU11_01990"
     CDS             complement(8427..9359)
                     /locus_tag="PLU11_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HPN57905.1"
                     /translation="MLTPILIFIVALTVLLVAAQFFTRAAEQIGGWLKLSPFVIGVFI
                     VGIGTSLPELISGIISTGKGLSEIVPGNIIGASISNILLITGLAVVLNKKSISLNSTY
                     IYIDLHFLLGSFFFFYLVAYDGVIRWTEAISGILIFLVYSFYLIRGGEKKTETESKSV
                     QTAFPVKALLLLIGSAVGIYFGADYTISSLEKIALGFNVPASIVALTLLSLGTTLPEL
                     AVNISAIRQGKAEMAIGNILGSCIFNTLVIPGATSLIADVKVPAELTGFSLPVFAACG
                     LLFYLLTQDKKISIWEGMMFICLYGLFILKISGG"
     gene            9585..11573
                     /locus_tag="PLU11_01995"
     CDS             9585..11573
                     /locus_tag="PLU11_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HPN57906.1"
                     /translation="MKCRPAALLLLSCFGYLLGLGQQPAPIWNADGKYGVVNSKGQVI
                     VPFEYDYISSYNQGLAIVAKNKKKGLVDVNGKMVIPVMYEDIYHCDNSGWPKPVKISG
                     KAGAINRKNQLVIPATYRFMDCFYNGLALVSDPKEETYGIIDSTGKLIIPFGIYQSLS
                     ADFNNGLVPAKKNDRWGYINKAGKEVIPFIYLHAFPFEKGRAEVTKGYDDIYINTKGE
                     EVNHYMTELRKQYEQLNYPLNGQIRYRKEGIGWCVMDTTGKELIPAGKYDDVDYFFEG
                     VAFVRKKNKWGAIDRQGNLVIPFYYMGKLRPFTDGLALLRNIEGEQVLINKKGEAVYG
                     KHIILDATKAATDYATGERLYQARKYQEAVPYLRSAADNGHEEAMYLLGLLYWKGFGV
                     LQDVDESMKWLKRASGNGSYNAREELKKIEKAGLPQVDEYKMGYDAFQLKKYKEAVSW
                     FNKAAAKGNMEAMFNLGMIYQLGAGTEIPANEKEAVGWYQKAADKGHVNSMNNLGQLY
                     LSEEGIQDFKLALQWFTKGADKGHSGCMYNIGVMYANGTGVTMQMEEAYKWFKKAADK
                     GMVEAKEAVKKIEKEGLVSTEVKNGDAAFDKKEYDKALTWYQQGADKGYSEAMYKLGL
                     IYELKHKTTLAREWYKKAIAGGHLKAKEALENLELTGF"
     gene            complement(11570..12103)
                     /locus_tag="PLU11_02000"
     CDS             complement(11570..12103)
                     /locus_tag="PLU11_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57907.1"
                     /translation="MNNLTLGPGTRLQHTQYGPGVIVALRYATYIISFINHGVKEIDK
                     TDDKLEEIIPENVSAEIETHSEVERSLLKILRLWGGITENVPLGEKWAKGTMLLQPFD
                     KTLKPKEIPVEDFFHKIVMLRDRLRVLEQNINSHKKLNDEDKVNLQQYITRCYGSLTT
                     FNVLFKDKEDWFVGEKS"
     gene            complement(12356..12709)
                     /locus_tag="PLU11_02005"
     CDS             complement(12356..12709)
                     /locus_tag="PLU11_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN57908.1"
                     /translation="MFLQLAHTKKPIYKDSRELVAECYRLTALFPKEEKFAMVSQIRR
                     AALSVHLNIAEGCSRRSEAERKRYFEIARGSVIEVDAAIGIACNLDFCKIESLSVIET
                     LILNCFKQLSALIGK"
     gene            13014..16379
                     /locus_tag="PLU11_02010"
     CDS             13014..16379
                     /locus_tag="PLU11_02010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPN57909.1"
                     /translation="MKQLPLLLGTALFFLPAAAQVKQPPANKQMEVQVVTQVQQVIGT
                     IALNGFNFENGKLSLAGWTREGTAFNNQPTYGHNVVIRREMDNPQNPFRVNITLGGDY
                     WKDLSLPIGHKGKYWIGSFENRGDSAQPFARTQGNTPMGTLLSPEFTISHDYISFLVG
                     GSSDMANEKIELIYEGQPSDAAGDDKNKSKNQGILINPLNASQAIAKFYKASGKGSSV
                     MRRVWWEVKTLKGKKARIKITDNSATGYINVDDIRFQEQSPDQTDIKIGTRLTVKQVF
                     QKEGNYYDWDFPLWGTIDTHTHPAAHIGFGNRLFHGEPVGTINKALADCEIDHGADIL
                     RTLTSSSGERIRGNIIRNAVAGVFDPGHRLGVMDPGNTWGSNEQTLAYWPIYNTKLHQ
                     QMWKDWLKRAYDGGLRMIVALTVHSQVFARISEGTRPDDDKSVGDKQIDYIKNVIAPA
                     CRDWMEIAYSPADMRRIMLNNKLAVILGSELDNIGNFDGKRAPTEAEVRAEIRRLYDK
                     GLRYMFPVHLLNNGFGGTAIKSDIFNLATKFNTGRAFDVELKDYKDGFSEYKLKAKFD
                     VLSDVPAAMIDANNKAIAYGGIAGAAAPVVVPAAAPLVLPFIPLITTAGTTVVGMAAP
                     LIPVLAGVAVNKWTNEWGISLDVIPVGNNYPAYPWKRYAGHINRQGLTTAGEIAVNEM
                     MRLGVMIDIDHAGRNTVDDYFRMAFAVPAGGYPLNSGHNQFTETRWTEAQLLPEAYDL
                     STVDGRKLYTNHYNHIAEKIANENDRKPDELIKIRQLGGMMGVGISGANVNALTNVIP
                     ASSRRYTESVVTNNNPGSSRSAAQLLLYAIEKMNGSGVCFGTDINGFAGLVGPRFGAN
                     SASAAGHDRYDIYNRRSFVYGQANQVRYTPFTDLSGNRFRGHLTGSYDDLKNNPDKFL
                     QNGKLFTEEQGDFMLALQIYYSMEERFKREAESGDTRNKIDWYLNHVVHSSYDKGRIR
                     EFIKGLIKGGNGENIGTDVFNGDVGTQQQISLKVSREKIFNNRTVDSRTGDVTSNSEK
                     NRRHKLLEAAFDYYKIMTRPGTNEPLIPASTLGKTWDYNIDGMAHYGMMADFFQDLKN
                     VGMKPKDMSVLFNSAEDFARMWEKCVRQSERVR"
     gene            16845..17285
                     /locus_tag="PLU11_02015"
     CDS             16845..17285
                     /locus_tag="PLU11_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPN57910.1"
                     /translation="MFIQKYFEEFELNHTRQTRGRTITETDIVIHAGQSGDFFPHHMD
                     EEWCKTQPFGRRIAHGTLIFTVAIGLTADFINEVSMTYGYERLRFIKPVFINDTIRVN
                     ITIKEKKDHKKPEYGLVTELVECFNQHKELVMLCEHILMVKKNV"
     gene            17287..17685
                     /locus_tag="PLU11_02020"
     CDS             17287..17685
                     /locus_tag="PLU11_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57911.1"
                     /translation="MAAIVLNGITWGHSRGITPLLALSQRYSELHPEVEIRWKKRTLQ
                     EFADFPIEKLTLEYDLLIIDHPWVGCAAATNCVLPLNEYLSPAYLNDQARNSVGQSHN
                     SYHYEGMQWALAMMQDPLRRCLKAIDERWR"
     gene            17825..19282
                     /locus_tag="PLU11_02025"
     CDS             17825..19282
                     /locus_tag="PLU11_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57912.1"
                     /translation="MKYFFIFFLFCSTRLLAQEQTYDIVTFTLPDGWSKGEENNAAIQ
                     YVKKEGTKWAQIAIYKNTTSLGTPEADFDSEWEKLAATPYQITALPIKTAAETVFGDW
                     KKTSGTGKWMFNGKEVITTVTTYTGYGACVSILCNRSDKKFAPAYDQLLSTIRLQSGN
                     TVAVQDPVPPPPADNGNEQTISNTQPVATGYKFNTTNFDDGWTSSIQEDWVQVEKGSI
                     KTMIHYPKEGTIFPSDPEPLIRAAWNILVAPRYSNLSNFSARYVETYNRPYFGSGYAT
                     ENSTGQSVFIVLFRRGGGWIEVVAPDNASFTREFGFNPESIVWGKLTEYSGGWVVTNS
                     AGTTINADPEVFNKLDNMTTYNKFAVAASDLAGTGKWNAGFSSNTYYANYYTGNYTGM
                     NTYTSSQWFDFKSGQQYHWELVAANTFNGQTNVAQAKSDGNFSSVNDWQLNFSNIEQR
                     SKTYDAYFTATKGGRVLWLNDANAPGSGIFTGYSK"
     gene            complement(19533..21056)
                     /locus_tag="PLU11_02030"
     CDS             complement(19533..21056)
                     /locus_tag="PLU11_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPN57913.1"
                     /translation="MYHFARFKVALLVFLFPIISTATIRLPYFISDGMVLQQNSDAAI
                     WGWASPGATVKVNTSWNKASYSTKAGSDGKWRLSVKTPVAGQVPTTVWDMSEDGSAAA
                     RMSPMLLEKTPTAGHGPYTITISDGKPVTIKDVLIGEVWLCSGQSNMEMAMKGFKDQP
                     IIGSNEAIFNSTNEHIRLYTVPRSVQRLPQDTSKQSAWKMAEPEAVSNFSATAYYFGK
                     MLYERLKVPIGLVNISYGGSPVEAFMDAETLKAFPEIKVPAPTDTSKLNNKNAAVLYN
                     GMLKPFLGYTIKGCIWYQGESNNDRAKQYETLFPAFVKQIREQSGQGDFPFFYAQIAP
                     YNYMNHAAVGATAYNSAYLRDAQRKALSVIPNSGMAVLMDIGEEFNIHPMDKLTGSKR
                     LAFMALGKTYGLKGFGYESPAYDAITISGNIVTVKFKNAPNGLSAFGKELKYFEVAGA
                     NKVFRPAKAMVNGGNIILSAPDVKEPVAVRYAFKDFVVGDLFSSEGFPVSSFRTDDW"
BASE COUNT         6162 a         4495 c         5216 g         5522 t
ORIGIN      
        1 tttattcaaa aatcaaaaaa gataaggaat atgactacct gatcatttca gccgtttcca
       61 ctcccattcg tctgctgtct tcctatctga ataaaatgca accatgaaaa acctgtgctt
      121 tttcttttta tacctgatta tttttccggc ctccgcccag gttattactc cgggacagga
      181 tacggccgct gccgtttatt atcagcaagc gggcgaatat gcagtacgga atgaacatgc
      241 agctgcgttc agatttttaa aagaagcgat agtgaatggg gccaggggag aggatgttct
      301 caccgacact gactttacag gcttacaaaa ggacctgaat ggctgggctg aaattgaagt
      361 cttgttgaaa agccagtatg tggccagtaa tccgggaatt tcaaagccgg acctggggta
      421 tgaactttgg cggatgtggg tggaggatca acgttaccgt acattaaagc gtaattataa
      481 attgcccgga aatccggtta ccgatatagc cctgcaccag caacagctta tccgggtgaa
      541 agagattatt aaaaaatccg gctggcccgc ttatagtgaa gtaggacggg caggcgggga
      601 tgcggtgttt tttgtttttc agcatgatga tgctaaaaac atgaaaaagg tactgccctt
      661 actgatcgcc gcggcaaaag ccggggaagc ggatatgagc aaagtcgcga tgatgattga
      721 ccggtacctg gcctatacgg agcaggtaca gctttacggc agccaggcat tccggagaat
      781 caaaccgggg caggaccgga aggatatacc gctgaccctg tatccgatag ctggtgaaga
      841 gaggatgttg gagcgacgta aagctttagg catggtcgat ttttttgaga attgcaggca
      901 attgggggtg gagtatataa gaattgagaa ccggacggat tataagccta tagtgattaa
      961 aaagaagtgg gtagatgcgg ggtatttatt gaccgaataa aagggttaat gacaaatttt
     1021 attcatttat ataatccatt gtgggggcag gagttcaacc ggatttacca ggtgttgaaa
     1081 aataaatgat tatttcgtga tgccctgttg caggataagg acttgatgat gcaatatgct
     1141 gcctttaaag aacgtttaac cggtcacaat ggcattagca gggaggcata aacaccatgg
     1201 ggaaacggaa attatcctgg aagatttacg aaatcaggtg atgacggatg ttgaactttc
     1261 ggcgaaccgg aagatgaatg gttaaacaaa caggaggggt gattcggatt tgcgtttaat
     1321 cttcgccgtt ctgtaaaatt ttcagtatcc ggtttaactt ttatactctt tctaagtcat
     1381 aaatatccta aaaccaaacc tatgcgatca tcgcagtata ctttattcat tcttctaatc
     1441 gtatttgcct cctgtaaaaa ggacaattcg aatccccccg cggccgaaac catgtatttc
     1501 ccgtccaaca cggacaccac ctgggagcgt aaatccatga gcagtctggg ctggaacacc
     1561 agtaatgtac aggcgctgaa agattatctc agtcagaaaa acagtaaatc ctttatgatc
     1621 ctggtcaatg gacggatcgt gatggaggaa tatttcaatg ggcatactgc ctcagatacc
     1681 tggcaatgga acagtgccgg aaagaccctg gtatctacaa ctaccggtat cgcccagcag
     1741 gaaggattgc tgaacattaa tacaaaagct tcgcaatacc tgggagcggg ctggacggca
     1801 gctccgttgg ccaaggaaaa ccttatcacc gtcaggcact tgttgaccat gacctccgga
     1861 ttaaatgatt cgaataacct ggttatccgc cctaacctta cctatatggc agatgcgggt
     1921 acccgttggt cttaccacaa tgtatttcag aagcttattg atgtggtagc agcaaccagc
     1981 gggcaaactt tcgataatta ctttaatgca aaactgaaga acaaactggg catggatggc
     2041 ttctggaatt atggtaccat ttttaccatc tatcatagca atacccgtag tatggcaagg
     2101 tttggtctga tggccttgaa taatggcaag tggaaagatg agcagatcat caaccagaca
     2161 ttcttcaatg aagcgatcaa ttcctcgcaa tcgatcaatc cttcttacgg gtatctttgg
     2221 tggttatgtg gcaagtccgg agcgatgata cctggcggtc agacttttta ccctggcaac
     2281 ctgattccca atgcccctac tgatgcgtat gcggctatgg gtgcagaaga tcagcggatc
     2341 tatgtagtac cctctaaaaa gatggtggtg atccggatgg gcgatgcctc tgatccactg
     2401 aatcccagtt tcgccgtatc gggctttgat aatgaattgt gggggaagct caacgcggtg
     2461 atcaattaat caaggattaa actaatttat caaccccgct attttgcggg gttttttgtt
     2521 gacaggcaat accggaaagt ggtagttgta atgtcaggga atggactaaa ttgtgtgcct
     2581 gatgaatgac agcataaaac tttccacgct cacgcaactg atccaggata cgatccagtc
     2641 acggtttgag ggagaagtat ttcaggtgtc cgcgaggatc atgaatgtaa agaaatatga
     2701 atctgcgcgc cgttgttacc tgacccttga agagtatgaa tatggcagca agatcactga
     2761 gatcagggcg gtattctggt ccaatgctta tagcgagatt gaaaaatttg agaaggcact
     2821 ccggcaacct ttcaaagacg gagcagagat tacctgtaaa gtaaaagtca ggtatcataa
     2881 agtgtttggg ctaaacctgg atgtattgca aattgatgtg gcacaggcac tgggcacact
     2941 tgaactggaa aggcagcaga ctctggagaa actggtaaag gagaacccgg aatatgtaca
     3001 gctgatcggc gggaattatc ttacctataa taacaggctg gaactacccc tagtcattag
     3061 ccgtattgcg ttgatcaccg cgccgaattc agacggccag cgcgattttc tgcaggagct
     3121 gaaggggaat aaacacgagt atcagtttgc cgtgactgaa tttctcacaa cgatacaagg
     3181 tgaaaatgcc catcagctga ttctggatca attgcgactg attgaaaagc agcaggaaaa
     3241 ttttgatatg gtggcgattg taagaggagg aggttcaatc agtgatttca ggccatttga
     3301 cgattatgca ctttcggctg cggtggcccg ctttccgata cccatcttta ctggtattgg
     3361 tcacgaccgt aatcaaagta ttgtggacct gatggccagg gaacaaaaaa caccgaccaa
     3421 ggtagcggct ttgatcgtgg agcataattt tgattttgag aatgcattga ttgatcttaa
     3481 agccaggatg agtgatgctg ttactcatca actcgaccgg gcagctgaaa aactggaaca
     3541 ggcgaagcgg atcatccggc tctcgagtcc gtgggatatt ctccgccggg gctttgcgat
     3601 tattacccat gaagataaaa tagtagtcga tccttcgctg attaaggaag gcatggaatt
     3661 acaaacacaa ttgatgaatg aaaccattca cagtacggta accaaaaaga caaaagatga
     3721 aaataaaact gacatatagt gaggccttta ataaactgga agaactggtg gaacagctgg
     3781 aagacgggac tataccattg gacaagctgg cggctacggt aaaacaggct aatgaatggg
     3841 tagcggtttg tgagggcaag ttgaggcaga ttcaggaaga ggtagaagcc gatgtagtag
     3901 cgaaaaagag taagggaaag aaatgagttt ttgctttcgt tttttaaaag ccttacaggc
     3961 aacgaaaccc tttttgatct tgtcatacaa acatgaagta ttttctttta cacctgcttt
     4021 tcctgtctgc actcaattcc ggagctcagc atcggaacag ttttgcattc accatacatg
     4081 ttcagccgga gatcacccga catcacaaaa actatgcgat tcagtggaat gaggcaggag
     4141 ctaccaccac cttcaatgca gggttacgcg cagctgtgca atatcgaatt gccggccgtt
     4201 ttttcgcaga ggccggattg ggatatgtac cccggcgtat gaataatacg ttgtattttt
     4261 tcaatcaaaa tgtaataccg cctccaaggc agagcccgac gcaggagctg gtcgggtata
     4321 aaaatatcac ttaccggact ctggagctac cgcttacagt gggtgtaatc tttttaaaat
     4381 taaaaaaagt ggaactgtac gctacaggcg ggtatactac taactacctg ctcagtgcgc
     4441 gatatgggaa ggggtccaat tcaaagtatg ccggggttta cagcaagcgg tattggcaag
     4501 gggaaagcct gctggcgggt ataggaaccg atatccgtat aaaagaaaaa cttttcttct
     4561 caacccgcct cagttactca ttacgaaatt cagtaaatgc ggatccttat ctatttagtc
     4621 agagttatga tgggctgagg atgacacacc agtttgtgca aggggcagtg ggtgttcggt
     4681 ggtcattaaa ctaaaactga ccgatattat cctcctgata ttttcaaaat aaacaactca
     4741 cacaaacaga taaacatcat tccatcccag accgtactct tattattctg tgtaagcagg
     4801 taaagcagta accccaaagt ggaaatcaaa attgacatta gtaatgaact ctggggcgac
     4861 cggcgttttg ccatacagga cccaaccggg atcaacattg atactgtgag gcacatgccg
     4921 taaaactgag gactatggga ggagcaggta tgaaagcttg ttttgatatt tttgagtggg
     4981 tgtatatttc ttttcacgaa acattaagct ttctcctata tttaaagaga aatggattcg
     5041 tttttactcc gaccgaatct caactttcta caaccagcac caatctttta ttcctgctat
     5101 ggcacaaaaa ttcttgtttt gccttttagt ttttcctgct ttatgggtta atagccagtc
     5161 ggattttctg gaccagctaa aaatctataa aataaacgac ccggtattga aagagatcca
     5221 gttctccgtg ttcaaaaagg atatggataa gaaaaagcca ttgttgcttt tcctcggagg
     5281 atccggcctg gaacccacgt tcaagtataa ccgggctgat cagcaggtgt attataccgg
     5341 cttttaccgg ttcagtcagt ataaagacag ataccatatt gtctttataa acaaggccgg
     5401 gataccgctg tatgattcgg tgaacaataa taatgaacag tataaagtaa ccccttttgc
     5461 ggaagccaat aacacagccg actggcgggc tcagagtgct tcgaaggcaa ttgattacct
     5521 gctgaaagta ctaccggtgg ataccagtaa tgtaattgtg gtcggccatt cacagggcgg
     5581 gcaggtggcg cccaaagtag cggtgctgaa ccggaaagtg aagaaagtag tgattatgtc
     5641 ggccaatgcc ctggatcata tttatgaccg gatgctgctg gccaggcaac aagcgctgaa
     5701 taaccagatg agttatgaag aagcacaata tgtggtggat agtctgttaa ctattcagaa
     5761 agccatctat cagtcgccgg ctgataccca acaaaccttt tggggagatc cgtttaataa
     5821 atggtacagt tactcgaagg aaactcccct ggagaatatg ctcaaactca atattccgat
     5881 actcctgatt gccggtggcc gtgatgtgga gggctcctat atagccaata ccgactatgc
     5941 ggcgctggaa ttcatacgaa aagggaagca gaacctgacc tataaagtgt atcccaatta
     6001 tgatcatatt tatggcgaaa cagcacgtaa atacggcgaa gagatcggac tggggtttaa
     6061 agccaatgcg gtggtggatg atgtttttaa gtggctgggt gagaagtagt gaatgtcaga
     6121 ctgtgatcat tcgtattccg aattcaacga agcacgattt ccccttgtct tactaatatg
     6181 aagagttacc tcagtttacg ggcttaatta taccatacgc aataccggta cattcgatcc
     6241 ataccttttc agccaggaca tggatggtat tccgctgaac caacaggttt tacgggggtc
     6301 ggtgggagtg aggtgggtat tataaatagc tgcacaggag tacccgggat tttgtatatt
     6361 aggagaagga tacgttgaca tttgaaaagt atccaatttc cactcacctc cataaactgt
     6421 aaatcatgaa aattctcagc gccatcctgt tatgttttat cttattctcc tgccagcaac
     6481 caacagaaaa aaaatcagct acaccagcag ttaccagttt tgatgaaacg gctgaaacag
     6541 ccgccatcct gcaagtcatc gaaaatgaga ccagttgttt tttcgccggc aattatgaat
     6601 gctgggccaa aaactggagt catgaacctt atgccatgca ggcctggaat aatgacgacg
     6661 gttccatgga tgcggctatt ggttgggaaa aaattaatgc acagggtaag aactggattg
     6721 aaaagtatta taaaaacggg aaagaagtga ttcaccctgt ggtgaagaag gaaaagccac
     6781 aggtaaagtt tttcaatgac cgcacggcct accttatctg gaaacagtat aatgccgata
     6841 aggagaataa atattataaa ctcagccagg aaaccaggat catggaaaag gctgccgacg
     6901 gctggaagat cgtcaacgtt tcggcctttt ggaattccaa taagaaaatt ccggtggata
     6961 gtttgaagct atagtattca tcagctcaca ataaacggat aaggttaacc gttaatactt
     7021 gtctttatcc aatctcctgt ttattcccct ttttattttc aaaccaacgg tataaccaat
     7081 gaaaagagta ttttttttgg ctggcattat tatgctcagc tgtgtagtca ctgcacaatc
     7141 gtttaaacag gcttttttca acgctgatat cagtaatttc tggaaagcgt atgataagat
     7201 cagggccacc acggatacgg cactgcagcg acagttaatc aatgatatat atatccgtcc
     7261 ggggtcagcc ggactgcagg aactgatccg gtaccggaat tatacagatg atgattttct
     7321 gactatgatc cgtgagagtc cggctttctg gaaatccttg cgtagtcaga ccctgaatac
     7381 ggaaaaactc tatccggtta tcgagatgga tattcaaaaa ttgaaaaccc tgtatcctcc
     7441 tttaaaaccc gcgacggttt atttgctgat cggtgctttc cgaacgtagg gtacgggcgc
     7501 aaatgggaaa gtgctgatcg ggagtgagta tgcgttggca ggaccagcaa cgaatatggc
     7561 tgatttacct cagcggttcc atgcttatta ccgggatttc aagccccgcg aaaacctggc
     7621 cctgctttgt acccatgagt atgtgcatac gcaacaaaat caaccggtgg acaatcttct
     7681 ttccaattgc ctctcagagg gtattgcgga gtttatttcc tgtaaggcaa ccggtatagc
     7741 ttccaacaca ccggccattg cctttggtaa acttaatacg gagaaagtgg tcagccagta
     7801 tgtgaaagac ctgtttgtga tgttcaatga ttataactgg atatggggtg aaaaccgtaa
     7861 tgaattcagg gtgcgtgacc tgggctatta tatcggctac agcattgcag agcggtacta
     7921 tgagttgtct gcggataaag ggaaggccat acgcgaactt attgaactgg actataccaa
     7981 cgatgcggct gtggaacacc tggtagatac cacgcggttc ttaccgaaaa agctatctga
     8041 actttatact gactatgata aaagccgtcc aagggtaatc gccctgtcgc cttccattaa
     8101 tggcacaaaa gatgtagcgc cggggttgac caggatcacc gtacatttct ccgagccgct
     8161 gaatgggatt aataccggaa ttgactttgg tccgctgggt cctgattatt ttccgaaact
     8221 gagccctgtc cgtgtatggt cggacgataa acgttcgata actcttgaag ctgatctgaa
     8281 acccggcaag acttatcagt ttatgattga taatacattc cggagagaag atcaggtaag
     8341 aatcaaaccg tttgtggtgg agtttagtac gaaggaatag gatttagagt tgagggttta
     8401 aaggaaagtc cctttttatg gagttatcat cctccggata tcttcaatat aaataatcca
     8461 tacagacaga taaacatcat gccttcccag atggatatct ttttatcctg ggtgagcagg
     8521 tagaagagta acccgcaggc ggcgaatacc ggaagggaaa aaccggtgag ttcggccggt
     8581 acttttacat cagcgattag tgaagtggca cctggtataa cgagggtatt aaaaatacag
     8641 ctacccagga tattgccgat ggccatttcg gcttttccct ggcggatggc tgaaatgttc
     8701 acggccagtt cgggtagggt ggtgcctaat gataaaaggg taagtgccac aatggaagcc
     8761 ggtacattga aacccagtgc tattttctcc agggaggata tcgtataatc tgctccgaaa
     8821 tagataccca cggcactgcc aatcaggagt aataaggcct tcaccgggaa cgctgtctgc
     8881 acagactttg attctgtctc tgtttttttc tctccccctc taatcaggta aaaactatag
     8941 acgaggaaaa tcagtatgcc cgaaatggct tcagtccaac ggatgacacc atcataggcc
     9001 acgaggtaaa agaagaagaa gctgccgagc aggaaatgca ggtcgatata gatataggta
     9061 ctgttaaggg aaatactttt tttgttcagt accaccgcca atccggtaat gaggaggata
     9121 ttggaaatgc tggccccgat aatattaccg ggtacgattt ccgataaccc tttgccggtg
     9181 gaaataatgc cggaaatcag ttcgggtaag gaggtcccga tccccacaat aaataccccg
     9241 attacaaaag gtgaaagttt taaccaaccc ccgatctgtt cggccgcccg ggtaaaaaat
     9301 tgagcagcga ccagtaaaac ggtcagtgcc acgataaaga tcaggatcgg ggtaagcata
     9361 tgactgataa aacaatgatg agaaaacggg ttagccctgc aataataatt aaatatctat
     9421 caagtcgtaa ctgaaagcaa ctcctcactc gattaaggat caaaaaacac cgggttgccg
     9481 gcattaattt ttccggatac cgtcttccgg gtatgttttt ctcatgtcta cctattataa
     9541 ttgcataaag gaaatttgct aaaccccgga ttaattgata aagtatgaaa tgtagacctg
     9601 ccgccctgct cctgttatcc tgctttggtt atctgcttgg actgggtcaa caaccagccc
     9661 ccatatggaa tgctgatggt aaatacgggg tggtcaatag caaaggtcag gtgattgtac
     9721 cttttgagta tgattatata agcagttaca atcaggggct ggctattgtg gcaaagaata
     9781 aaaagaaggg gttggtcgat gtaaacggga agatggtgat tccggtcatg tatgaagata
     9841 tctatcattg tgacaacagc ggatggccta aacctgttaa aataagcggg aaggccgggg
     9901 ccatcaaccg gaaaaaccag ctggtgatac cggctactta ccggtttatg gattgttttt
     9961 ataatggact ggccctggtt tcggacccta aggaagaaac ctatggaatt atcgacagta
    10021 ccgggaagct gatcattcca tttgggattt accaatctct ttctgcggat tttaataatg
    10081 gactggtacc cgcaaaaaag aatgaccggt gggggtatat taacaaggca ggaaaagaag
    10141 tgattccctt tatttacttg catgcgtttc cttttgagaa gggcagggct gaagtgacta
    10201 aaggatatga tgatatctat atcaatacca agggagaaga ggtgaatcac tatatgacgg
    10261 aactccgcaa acagtatgag caattaaact acccgcttaa tggccagatc aggtacagaa
    10321 aggagggcat tggctggtgt gtaatggata caaccggtaa agaacttatc cctgcaggca
    10381 aatatgatga tgtggattat ttttttgagg gagtcgcttt tgtcaggaaa aaaaataaat
    10441 ggggcgctat cgatcggcaa ggtaatctgg ttattccttt ttattatatg ggtaaattac
    10501 gtccttttac tgacggtctg gccttactca gaaatataga aggagaacag gtgctgatca
    10561 acaagaaagg agaggcggtg tatggaaagc atattatact ggatgccact aaggcggcta
    10621 ctgattatgc tacaggggag cgtttatacc aggccagaaa ataccaggaa gccgtacctt
    10681 atctgcgcag tgcagctgat aacggacatg aagaagcgat gtacctgctg ggactgcttt
    10741 attggaaggg atttggagtg ctgcaggatg ttgatgaatc gatgaagtgg ctgaaacgtg
    10801 cttccggtaa cggaagttat aatgcaaggg aggagttgaa aaaaatcgaa aaggccggtc
    10861 tgcctcaggt ggatgaatac aaaatggggt atgatgcttt ccagctgaaa aaatataaag
    10921 aggcggtcag ctggtttaac aaggctgccg caaaagggaa catggaagcc atgttcaatc
    10981 ttggaatgat ctatcagttg ggagcaggta ctgaaatacc ggccaatgaa aaagaggcgg
    11041 tgggctggta tcaaaaggca gccgataaag gacatgtgaa ttccatgaat aacctgggtc
    11101 aactgtattt atccgaagaa ggtattcagg attttaaact ggcccttcaa tggtttacca
    11161 aaggggcaga caaagggcat tccggatgca tgtataatat cggggtgatg tatgcaaatg
    11221 gtacgggtgt gacaatgcaa atggaagagg cttataaatg gtttaaaaag gcggcagaca
    11281 aagggatggt ggaagctaaa gaagcggtta aaaaaattga aaaggaggga ctcgtatcaa
    11341 cggaggttaa aaacggggat gcggcttttg acaagaaaga atatgataag gcgctcacct
    11401 ggtaccagca gggagctgat aaagggtaca gtgaagccat gtataaactg ggactaatct
    11461 atgagcttaa acacaaaacc accctggcca gggaatggta taagaaagcc attgccgggg
    11521 gccatctgaa agcgaaggaa gcgcttgaaa atctggagct gacggggttt tagcttttct
    11581 ctcccacaaa ccaatcctct ttatccttaa acaatacatt gaaagtggtg agtgaaccat
    11641 aacagcgggt gatatactgc tgcaggttca ctttatcttc gtcattcagt tttttatgac
    11701 tgttgatatt ctgttccagc acccgcagcc ggtcgcggag catgacgatc ttgtggaaga
    11761 aatcctccac cggaatttct ttgggcttaa gtgttttatc aaaaggctgc agcagcatag
    11821 tgcctttcgc ccatttttcc ccaagtggta cattttcggt aatgccgccc cagagccgca
    11881 ggatcttcag caacgaacgt tccacttccg aatgggtttc gatttcagcg gatacattct
    11941 ccggaataat ttcttccagt ttatcatctg tcttgtctat ttcttttacc ccatgattga
    12001 taaaggagat gatatacgtg gcataccgga gggcgacgat tacgccggga ccgtattggg
    12061 tatgttgcag gcgggtgccg gggccgaggg tgaggttgtt catggtggga aattactgtg
    12121 atgtcgggaa ttgaccaaaa ggtcggatga gtaatgagta atcagtaatg agtagctcgg
    12181 gaaggggctt attggcagag gcaggttgag ggtgggatga gaaatgagta gcccgggaag
    12241 aggctcttcg ggtagtggca ggataaaggt cggatgaaaa atgagtaaag agtagttcct
    12301 ggaaactaaa ataattccgg ggcaggttgg tagtggggca acgctaataa tccggctatt
    12361 ttcctataag ggctgaaagc tgcttgaaac agttcagtat cagtgtttca attacgctta
    12421 aggattcaat tttacagaag tccaaattac aagcaattcc tattgctgca tctacttcaa
    12481 ttactgatcc ccttgcaatt tcaaaatatc ttttacgttc tgcttcggaa cgcctggagc
    12541 agccttctgc aatattcaaa tgtactgaaa gggcggctct ccggatctgt gaaaccattg
    12601 caaatttttc ttctttagga aataatgcag taagacggta gcattcagct accaactccc
    12661 tgctatcctt gtaaattggt ttttttgtgt gggcaagttg taaaaacata gtttaaggtt
    12721 aattccttaa atatattatt tccaccaatc gaaattttat ccgtcgccat ttttattaaa
    12781 aactcttcca cttacctgtt cttactgaat ataatcctct tcccgggcta ctcattactg
    12841 attactcatt actcattctt ctctcaacct gtctctgcca ataagcccct ccccgggcta
    12901 ctcattactg attactcatt catttacagg ctgtttctgc caataagaat tcccccgcta
    12961 tttcatttga aaaagtaaaa ccgtatattg ttctcctaac cattacacct caaatgaaac
    13021 aactaccctt actacttggc acagcccttt ttttcctgcc agctgctgct caggttaaac
    13081 aacccccggc caacaaacaa atggaagttc aggtcgtcac ccaggtgcag caagtcatcg
    13141 gcacgattgc gctcaatgga tttaattttg aaaacgggaa attgtccctg gccggctgga
    13201 cccgtgaagg caccgcattt aataatcaac cgacctatgg acataatgtg gtgatccgcc
    13261 gggaaatgga taatccgcag aatccgttcc gggtgaatat tacattgggc ggagattatt
    13321 ggaaggatct ttcattgcct atcggacata aaggaaaata ctggattggc tcttttgaaa
    13381 acagaggtga tagtgcgcag ccatttgccc ggacacaggg caacacacct atgggcacct
    13441 tgctctctcc ggagtttact atctcacacg attatatcag ctttctcgtg ggcggcagta
    13501 gtgatatggc gaatgaaaaa atagaactga tctacgaagg acaaccttcg gatgccgctg
    13561 gtgatgataa aaacaagtcc aaaaatcagg gaatactgat taatccttta aatgccagcc
    13621 aggctattgc taagttttat aaggccagcg gaaaaggaag tagtgtaatg cgccgtgtct
    13681 ggtgggaggt gaaaacattg aaaggaaaga aggcgagaat aaaaattacc gataacagtg
    13741 ctaccggata catcaacgtg gatgacatcc gttttcagga acaatccccc gatcagaccg
    13801 atataaagat cggcacccgt cttaccgtta agcaggtgtt tcagaaagaa gggaattatt
    13861 atgactggga tttcccgctg tgggggacca ttgatacaca tacacaccct gcagcacata
    13921 tcggttttgg taaccgcctc tttcatggtg aacccgttgg cactattaac aaggcactgg
    13981 ccgattgtga aatcgatcac ggagccgata tattgcgtac cctgacgagt tcctccggtg
    14041 aacggatccg cggaaatatt atccgtaatg cggtggccgg tgtgttcgac ccaggccatc
    14101 gcctgggtgt tatggatccc ggtaatacct ggggatccaa tgaacaaacc cttgcctact
    14161 ggccgattta taataccaaa ctgcatcagc agatgtggaa ggactggctg aaaagagctt
    14221 acgatggtgg gttacggatg attgtggcgc tgactgtaca tagccaggtg tttgcccgta
    14281 tcagtgaagg gacccggccg gatgatgaca aatcggtggg ggataagcaa attgactata
    14341 taaaaaacgt gattgcacca gcttgccgtg actggatgga aatcgcttat tctcctgctg
    14401 atatgcgccg gatcatgttg aataataaac tggctgtgat cctgggcagt gaacttgata
    14461 atatcggcaa cttcgatgga aagcgggcgc caactgaagc ggaagtgaga gcggaaatcc
    14521 gtcgtcttta tgataaaggg ttacgctata tgttcccggt gcatttactg aacaatggct
    14581 ttggtggaac cgccatcaag tctgacatct ttaacctggc caccaagttt aatacgggca
    14641 gggcttttga tgtggaacta aaggattata aggatgggtt ttcggaatat aagctcaaag
    14701 ctaaatttga tgtgttgagt gatgtacctg cggccatgat cgatgccaat aataaagcga
    14761 ttgcttatgg gggtattgca ggtgctgctg ccccggtagt ggtacccgct gccgctcctt
    14821 tggtattacc ttttattccg ctgatcacga ctgctggtac aacggtagtg gggatggcgg
    14881 ctccgctgat tccggtcctg gccggagtgg cggtaaacaa gtggacgaat gaatggggca
    14941 tttcactgga tgtgataccg gtgggaaaca attacccggc ttatccctgg aaacgttatg
    15001 cgggtcatat caaccggcag ggattgacaa cggctggcga aatagccgtg aatgaaatga
    15061 tgcggttggg tgtgatgatc gatattgatc atgccggcag aaatacagtg gatgattatt
    15121 tcaggatggc ctttgccgta ccagcaggag gttacccgct taacagtggc cataaccagt
    15181 ttaccgaaac ccgctggacg gaagcgcagc tactacccga agcctatgat ctcagtacag
    15241 tggatggccg gaaattatac acgaatcatt ataatcatat agctgaaaaa attgcgaatg
    15301 aaaatgaccg caaacccgat gagctgataa aaatcaggca gctgggcggt atgatgggtg
    15361 tgggcatctc aggagccaat gtaaacgcgt taacgaatgt aatccctgct tcctcccggc
    15421 gctataccga atcggtggtg accaataata atccgggcag cagccggtcc gcggcccagt
    15481 tattattata tgctattgaa aaaatgaatg ggagcggggt ttgttttggt acggatatca
    15541 acggctttgc cggactggta ggtccgaggt ttggggcaaa cagcgccagt gcggccggac
    15601 atgaccgtta tgatatttat aaccgccgca gttttgtata tggacaggcc aaccaggtgc
    15661 gctatacccc ctttaccgat ctttctggta accgtttccg cggccatctg accggctcct
    15721 atgatgatct gaagaataat ccggataaat ttttacagaa cggtaaactg tttaccgagg
    15781 aacaaggcga ttttatgctg gccttgcaga tttattattc catggaagag cggtttaaac
    15841 gcgaagctga atccggtgat accagaaata agattgactg gtacctgaat catgtggtac
    15901 acagcagtta tgacaaggga cggatcaggg agtttatcaa aggattaatc aaaggcggga
    15961 acggagagaa tatcggcacg gatgttttta acggagacgt aggtacccag caacagattt
    16021 ccctgaaagt aagccgcgag aaaattttca ataaccgtac ggttgacagc cggaccggtg
    16081 atgtaacctc caattccgaa aagaaccgtc gccacaaact gctggaagca gcgtttgatt
    16141 attataaaat aatgacccgg cccggtacca acgaaccgtt gattccggcc tccaccctgg
    16201 gtaaaacctg ggattataat atcgatggca tggcgcatta cgggatgatg gcggatttct
    16261 tccaggacct gaagaatgtg ggcatgaaac ccaaggatat gtcggtgctg tttaactccg
    16321 ccgaagattt tgcccggatg tgggagaaat gtgtgaggca gagcgagcgg gtgaggtagc
    16381 tgctcgggga gaggcgttca aggtagaaac agactgaaag tagaatgagt aatgagtaat
    16441 cagtaatgag tagctcgggg aaagacctta cgttttgaag caggtttggg gagggggata
    16501 agtaatgagt aatcattaat tatccccttt ttcagcctgt ttctaacttt aatacctcct
    16561 cccgagctac tcattactga ttactgatta ctcatccgaa attgtaaaat tgtgccaaaa
    16621 tgtccctgtt tacttagggg agacttctaa gaaagcaata aatattccat caaatcagta
    16681 aaaccttcca tttaacgaca aggggttgca gggtactttt aaagagaatt tcaagcataa
    16741 gcagcaagca gaaacggcca gccctttgta acgaccttcc ggtcgcttac aaaggggccc
    16801 tgaccggctc ccgcccctgc acaaaccgaa acgattgcca caccatgttc atacagaaat
    16861 acttcgagga attcgaactc aatcataccc ggcagacccg gggccggacg atcaccgaaa
    16921 cagatattgt gattcacgcg ggacaatcgg gcgacttttt cccccatcat atggacgaag
    16981 aatggtgcaa aacccagccc tttggccggc ggattgccca tggcaccctc atctttactg
    17041 tggcgatcgg actgaccgct gattttatca atgaagtatc catgacctac ggttatgagc
    17101 ggctccgttt tatcaaaccc gtgtttatta atgataccat tcgggtaaat attactataa
    17161 aagaaaaaaa agaccataaa aaaccggaat acggcctggt gaccgaactg gtggaatgtt
    17221 tcaatcaaca taaagaactc gtgatgttgt gcgaacatat cctcatggta aaaaagaacg
    17281 tttaatatgg cagccatcgt tttaaatggt atcacatggg gacatagccg gggtattact
    17341 cccctgctgg cgctgtccca gcgatacagt gagctgcatc ccgaagtgga aattcgttgg
    17401 aagaaaagaa cattgcagga gttcgcagat tttccgatcg aaaaactcac tcttgaatat
    17461 gacctgttga tcatcgatca tccctgggtg ggttgcgctg ccgccacgaa ttgtgtgctg
    17521 ccattaaatg aatatttatc gccagcctat ctgaatgacc aggcccgtaa ctcagtcggc
    17581 caatcgcata acagttatca ctatgaagga atgcaatggg cactggccat gatgcaggat
    17641 ccactcaggc gttgcctgaa agcgattgat gaaaggtgga gataggttga aatgtttaca
    17701 agttgacaag tggtgctctt agcaggttgc cttatcaacc tgccaacctt ccataaaaat
    17761 cctctttttc cgggatacgc ccctctttag aatatttcat ctttgagcag taaatatgcc
    17821 cgacatgaag tacttcttta tatttttcct tttctgctcc acccgcctgt tagcccagga
    17881 acaaacctat gatatagtta ctttcaccct gcccgatggc tggagcaagg gggaagagaa
    17941 taatgcggcc attcagtatg taaaaaaaga gggaaccaaa tgggcacaga ttgccattta
    18001 taagaacacc acttcacttg gaaccccgga agcggatttt gacagtgagt gggaaaaact
    18061 ggctgccacc ccttatcaga ttacagccct tccaataaaa acagctgctg aaaccgtttt
    18121 tggcgattgg aaaaaaacca gtggcaccgg caaatggatg tttaatggta aagaagtcat
    18181 taccacagtg actacctata ccggttacgg agcctgtgtg agtatactct gcaatcgctc
    18241 ggataaaaag tttgctcccg cttatgatca gctgctgagt acgatccggt tgcaatccgg
    18301 gaacacagtt gctgtgcagg accccgtacc tccgccaccg gctgataatg gcaatgaaca
    18361 gacgatcagc aatacgcaac ccgtggctac cggttacaag ttcaatacga ccaattttga
    18421 tgatggctgg accagcagca tccaggaaga ctgggtacaa gtggagaaag gatccatcaa
    18481 aacgatgatc cattatccca aagagggaac gatcttccct tccgatcccg agccgctgat
    18541 cagggctgcc tggaatatac tcgtggcacc ccgttacagc aatctgagta atttcagcgc
    18601 ccgatatgtg gaaacttata accgtcctta tttcggttca ggctatgcca cggaaaacag
    18661 caccggtcag tcagttttta ttgtgctctt ccgcaggggc ggcggctgga ttgaagtggt
    18721 agcaccggat aatgccagct ttacccgtga atttggcttc aatccagaat ccattgtctg
    18781 gggaaagctg actgaatatt caggtggctg ggtagtgacc aacagtgccg ggacaacaat
    18841 taacgctgat ccggaggttt ttaataaact ggataatatg acgacctaca acaaatttgc
    18901 ggtggccgcc tccgacctgg ccggcaccgg aaaatggaat gccggttttt ccagtaatac
    18961 gtattatgca aattattata ccggaaacta taccgggatg aacacctata cttcttccca
    19021 gtggtttgat tttaaaagcg ggcaacagta tcattgggaa ctggtggcgg ccaatacttt
    19081 caacggccag actaatgtgg cacaagcgaa gtctgatggt aacttctcct ctgtaaatga
    19141 ctggcagctg aatttttcaa atattgagca gcgctcaaaa acatatgacg cttatttcac
    19201 cgccactaaa ggagggaggg tgctctggct gaatgatgcg aatgcaccgg ggtcggggat
    19261 ttttacggga tactcgaaat gagtaatgag taatcagtaa tgagtagctg tggaagagtt
    19321 caattgagct gtaagaggtt gagtaaggat gaataatgag taatcagtaa tgagtagctg
    19381 tggaacaccc agattgttca gggtcagtat taaagtggaa ggttttaaaa aagaaacgaa
    19441 aaatctttac agctttcaaa ctgattctaa actgtaaaga tttttccaga gctactcatt
    19501 actcattatt cattactcat tgtccccctt cctcaccaat catctgtcct gaaactactc
    19561 accggaaacc cctccgaact aaacagatcc cccaccacaa aatccttaaa tgcataacga
    19621 accgctacgg gttcctttac atcgggagca gataagataa tattgccgcc gtttaccatc
    19681 gctttcgccg gacggaatac cttatttgcc ccggccactt caaagtactt cagctctttg
    19741 ccgaaggcag ataaaccgtt tggcgcattt ttaaatttaa ccgtgacaat attaccactg
    19801 atcgtgatgg catcataagc gggactttca tagccaaatc ccttcagccc gtatgtttta
    19861 ccaagtgcca taaaagcgag tcgtttactg ccggtcagtt tatccatcgg atggatatta
    19921 aattcctccc cgatatccat cagtacagcc atgccgctat tgggtataac agataaagcc
    19981 ttacgttgtg cgtccctcag gtaagctgaa ttataagccg tagcacccac ggccgcatgg
    20041 ttcatataat tatacggcgc aatctgcgca taaaagaaag gaaagtctcc ctgtccgctt
    20101 tgctcccgga tctgtttaac aaaagcagga aatagcgtct catattgttt cgcccggtca
    20161 ttattgcttt cgccctggta ccagatacaa cctttgatgg tataaccaag aaagggtttc
    20221 agcattccat tgtacaatac cgcggcattt ttattattga gtttgctggt atcggtggga
    20281 gcggggactt taatttcggg aaaggctttc aatgtctctg catccataaa tgcttccacc
    20341 ggagagcctc cgtaactgat attgaccaac ccgatcggta cttttaatct ttcatagagc
    20401 atttttccaa aataataagc cgtggcactg aaattactca ctgcttcagg ctccgccatt
    20461 ttccaggccg actgtttgct ggtatcctgt ggtaaacgtt gtacggaacg cggaacggta
    20521 tagaggcgga tatgctcatt cgttgaattg aagatggctt cattactgcc aataatgggc
    20581 tggtctttga atcctttcat cgccatttcc atattactct gcccgctgca caaccatact
    20641 tcaccaatca gtacatcctt gatggtaacc ggcttcccat ccgaaatagt aatagtatac
    20701 ggaccgtgtc ccgctgttgg agttttctcc aacagcatag gagacatcct cgcagccgca
    20761 gatccatcct ccgacatatc ccatacagtg gtaggcacct ggccggccac cggggtcttc
    20821 acggataaac gccatttgcc gtcggacccg gcttttgtac tatagctggc tttattccat
    20881 gaagtattca ctttcaccgt agcgcccgga ctggcccagc cccagattgc ggcatccgaa
    20941 ttttgctgca ataccatgcc gtccgaaatg aaataaggaa ggcggatagt ggcggttgag
    21001 atgatgggaa acaggaatac aaggagggca actttaaagc gggcgaaatg atacatggca
    21061 tggtcgtttt tgttggcacc aatgtaggga ttttggcggg gaaaagcatc atgtagcagc
    21121 ggatgagtaa tgagtaatca gtaatgagta gctgtggaag acgctattta aattgtaaca
    21181 ggcttaaagt ggatgagtaa tgagtaatca gtaactgtgg aagactcaaa atgatatgaa
    21241 atcttgctaa agcttaaaaa aagtaatttt gggcttcgaa atgcataaag tttataaaat
    21301 ataagttctc ttccccactc attactcatt actcattttt cttactttag ctcccgatac
    21361 gccacttatg atcaaggaca aagacaagga caaca
//
LOCUS       DAOPMW010000019        21069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2361_length_21069_cov_11.890597, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000019 DAOPMW010000000
VERSION     DAOPMW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_21069_cov_11.890597"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLU11_02035"
     CDS             complement(<1..227)
                     /locus_tag="PLU11_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HPN57914.1"
                     /translation="MSAESNEKEVLLKIELDTFSNAIAMDMGLKIVELAKSRNQMIAV
                     EISRLNQSVFLYVEDGLPADKHNWLRRKANVA"
     gene            complement(308..1276)
                     /locus_tag="PLU11_02040"
     CDS             complement(308..1276)
                     /locus_tag="PLU11_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN57915.1"
                     /translation="MINWGIIGCGDVTEVKSGPAFNKVPGSRLIAVMRRDTTKAADYA
                     KRHQVDRWYADADALIQDPGVNAIYIATPPDAHEKYTLAAIKAGKPVYVEKPMALNYA
                     AAKNMAAVAAAAGIKLTVAHYRRQWPLFLKLKSLLAQQVIGEIRFVRLQFTRQAPAAA
                     ELTDTKNAWRVNPAISGGGLFHDLAPHQLDILYYLLGDAEKIAGMASNQGGHYAADDI
                     VTGMIRFTNGVLFSGTWCFTAAEASDDCEIIGSTGTIHFSFFSKQEIRLTLDGQTTEF
                     TFDPLAHVQQPMIAEVVNYFSGNASNPCSGEEGALIMSWMEQMTGK"
     gene            complement(1376..1681)
                     /locus_tag="PLU11_02045"
     CDS             complement(1376..1681)
                     /locus_tag="PLU11_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN57916.1"
                     /translation="MKTKKNNLITLDQLIEKNYGKKGTPKRDKFEAGYENFKIGALLQ
                     MARVEKGMTQQELAEKTGTTKSYISRIENNIKEVRLSTLHKIVEQGLKGKVRLSIEL"
     gene            complement(1662..2012)
                     /locus_tag="PLU11_02050"
     CDS             complement(1662..2012)
                     /locus_tag="PLU11_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPN57917.1"
                     /translation="MTNTQKFRTIVFFKDYFELFYEQQREKVKAKIVWTLKLIETLPV
                     IPETYLKHIENSKGLYEVRVQLGSDIFRIFCFFDEGQLIILINGFNKKTKKTPSSEIQ
                     KALSIKQEYENEKK"
     gene            complement(2254..2838)
                     /locus_tag="PLU11_02055"
     CDS             complement(2254..2838)
                     /locus_tag="PLU11_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57918.1"
                     /translation="MKKRLIVLLIIILSISCNNNELTDSGEIPVKGTDTYSHLGITRQ
                     KDSLIAALSEMKTEKLDYSAEAYWKIIQQGEKMIPVLLECLTDTHPTTIYNNCKKGKL
                     NVGEVSYFAQEELADFPAFLITQTQYDLIENDCWNFYTYLFNDKNKNEYQRKARNFYT
                     SNRKINYQYKKFSKKEMTPCRKLYKIEGRLVWNG"
     gene            complement(3170..3853)
                     /locus_tag="PLU11_02060"
     CDS             complement(3170..3853)
                     /locus_tag="PLU11_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57919.1"
                     /translation="MKLALLILLLFGLNSSAQIAEDLNSQTKKHLLAVKDGLTTTQYQ
                     LFLTLISKRDTASLMQRFRELAPINIVLIEGYHFKTGKYGIFEEYHFTPFGNSWESKA
                     YLNNHPADKLYSSVYLDSSLLASPAFRIDSLYPRVYQMGMGCGVTRQMQRIARRLQKK
                     AKKDGMVLESYCRIETIFLKSENRIITRFLVPVAGKRKTLIRSGFGTEIAKAGLEYWL
                     FRSSYFTGD"
     gene            complement(3911..4462)
                     /locus_tag="PLU11_02065"
     CDS             complement(3911..4462)
                     /locus_tag="PLU11_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57920.1"
                     /translation="MNRLFHIAVLLTILFIVETSCVNKHITRDDTINNYEKNSKQIME
                     LAAYFLKLKPDSNEVFFKPSKNRYTISIAQPYWAISPDYPYNAGRDMRMDSEQMRSFL
                     KTLDWTTNTVITLKKMLKKTNCLSISTQGDYIQLDFAAGDICAFSYLITEKPFSDSTI
                     ENDSKKGVTFLSKYARIGSACAL"
     gene            complement(4622..5740)
                     /locus_tag="PLU11_02070"
     CDS             complement(4622..5740)
                     /locus_tag="PLU11_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPN57921.1"
                     /translation="MSNTITIQQIELYKLSIPLIEPFITSLGTDYTADNVLIRVLTKE
                     GIVGFGECSPYMPINGESQDTCMVVGELFANALIGHNALDIEGAVNLMDSIIYANGSI
                     KSAFDIALYDIASQNAGVPLYQFIGGKNNKTIITDYTVSLAEPDVMAAQAIKILAQGY
                     PAIKVKLGKDGKKDVVRMKAIRAAVGNDIPLRIDANQGWGVEEAIETLNALAPLDIQH
                     CEEPIARWNYLSLPRVKKESPIPIMADECCGDDHDAERLIALGACDYFNIKLGKSGGI
                     FKALKMARMAEAAGLHLQVGAMIESRLAMTAFAHFALSSPNIVHFDFDTALMLREDPV
                     TGGIVYKANGVVEVPEAIGLGATISEEWLGKWEKVVAK"
     gene            complement(5750..6376)
                     /locus_tag="PLU11_02075"
     CDS             complement(5750..6376)
                     /locus_tag="PLU11_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57922.1"
                     /translation="MGKSTLAQYPSFSIGTDLGAIRNWPKGQQFTGLNHSVQLDFHLA
                     EKDGIQVNFSYCSFGKYNNVLRAKATDAVTLPQQIQYTNAGKLRFRLLSVGWKHYLHG
                     NPQLETGWSLYGKAGFGLLMGRVENSHNRTIDTALYQIPVREGTANFKRMTLDLALGW
                     EKPLGADFYFYTEARVTVPSPGYPSPFLLVNDNAPFTTLLCGGLRILF"
     gene            complement(6749..8914)
                     /locus_tag="PLU11_02080"
     CDS             complement(6749..8914)
                     /locus_tag="PLU11_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPN57923.1"
                     /translation="MKKIFLTIVLFLSLALKSMADEGMWLPLLLGQQVYNDMVKKGLK
                     LTKDQLYNINKASLKDAIIIFGGGCTGEIVSAEGLIFTNHHCGYNAIASASTMEHNYL
                     QNGFYARNKGEEIPTTLSVQFLLRIDDVTKEVMDSLQGLSGTERVAKQNAVLAAINKR
                     MSDAGQFIETRVSPLFKGNQFLAFVYQRYSDIRLVGTPPESVGKFGGDTDNWEWPRHT
                     GDFSVFRVYADADGKPAKFAEGNKPMKPKWYLPVSLRPLKDGDFSMIWGYPGGTNRYE
                     SSYGIKIATDINNPTLVKLRDARLKYMFEEMKKDPAVKLQLASSYAGIANYWKFFDGE
                     TKQLLKYDVFGQKQTAETKFNAWAKGKPEYENLFADWGKAYDAWRPYSKHRVYIVEGV
                     MGSPLIAFAGSLMQVEAAMVKTGSSKADIAKAVEAANNQRTNFLKDENKTSDENILAA
                     VTRMFYEDVDKQQHPLGFYGTLKNSYGALNDESTYKKYAADVFSKTMIFDDAKWAAFV
                     ANPDAVTLQSDLAFAHASAFLVNYNTKYAPFSQQFNTKNAEFGRLYLKGVMEMDPAKA
                     KMMYPDATFTMRVSYGNVKSYRPRDAVFYDYVTTSKGLLEKYKAGDYEFDLPANQIEL
                     LKKKDFGQYMDPTRKDLVIGFITTNDITGGNSGSPVINGRGELIGLAFDGNYEALSHK
                     LAFDKDLNRTICVDIRYVLWCIDKLGGGANIIKELKLMK"
     gene            9008..10351
                     /locus_tag="PLU11_02085"
     CDS             9008..10351
                     /locus_tag="PLU11_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPN57924.1"
                     /translation="MQKNQLAAVILAALFLVYSCGKKGNTPNPPPNPVPGCSSGNAPA
                     NGSFVTSTSVTLSWNSVGTASAYDIYLGTTAVPTTLIAANVTGTSTTYTIPATPNTTY
                     YWYALPKNTGGSATGCTGTVQSFTYIVITPPASLGYYVVGYMPSYRVLADIPDVKFRM
                     TNVVVYAFYGVNGSGTISAPTSGSTLAATVTKARANNAKILLGINDGTGDGKTNFKNM
                     ASTAAGRTNFIKSVMAVVRANNLDGVDMDWEFPSTSDGTDVTFTALMKELSDTLHRDA
                     RYYLSAAVTAGKYAGGIRDAIKNEIFPYVDFFNIMAYDDFSTSTPYRHHSDYTLATVC
                     LNYWLTTRGMPAAKAVLGIPAYGRPSGMTQSGTIKTYKDILAAGGNPQYDSATVSTTT
                     FPSYTVYYNGQYTTKRKAKLAKDIAAGVMFWEKGQDAMDGNSLLKAACDTIGRTY"
     gene            complement(10536..11345)
                     /locus_tag="PLU11_02090"
     CDS             complement(10536..11345)
                     /locus_tag="PLU11_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57925.1"
                     /translation="MNKALILAALTLLLSFQSNAQAVKTLKKVITLEITEEGGSNGAA
                     VAWHPIQKKYYAAMAGNSEFPLVVFDAQGKKVFEDGITTQYDVRGLWYNPFAKALQAN
                     GYSDYGWGQYNLFEGGKPGEIITFHEGQLQPTEQSVGAYDPAKNLLYFLDEDGNLEQY
                     SVKDTAYIKTINLQLGVTKKDDTGLTDNYDVIEEYNSASIIYTGIKGSEIGLLNIETQ
                     EVELYDVSNGYLVSKLKLPEGTATYEMFNFSYCNGMYWFFNQELRTWTGCK"
     gene            complement(11409..12410)
                     /gene="obgE"
                     /locus_tag="PLU11_02095"
     CDS             complement(11409..12410)
                     /gene="obgE"
                     /locus_tag="PLU11_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPN57926.1"
                     /translation="MEKSNFVDQIRIFCRSGHGGAGNKHFMRNKFTALGGPDGGDGGR
                     GAHIILRGNSNLWTLLHLRYYKNVLAEDGERGGANNCHGRDGKDIIIDVPLGTIARDE
                     ESGGQEVEILEDGQEVIWLPGGKGGLGNSHFATPTNQAPEHAQPGLPGVEGWKVLELK
                     VLADVGLVGFPNAGKSTLLSVITAAKPKIANYAFTTLVPNLGMVEYRDGKSFCIADLP
                     GIIEGAAEGKGLGHRFLRHIERNSVLLFLIPADCDDHKKEYDILVNELEQYNPELLHK
                     QFIIAVSKSDMLDDELKQAIEKELPADVPHVFISSITNKGLTELKDMLWKILNEPVE"
     gene            complement(12436..>12642)
                     /locus_tag="PLU11_02100"
     CDS             complement(12436..>12642)
                     /locus_tag="PLU11_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57927.1"
                     /translation="EICVYLRFHLRHLRDCILVVEGLRDEGKSRSYQQHPLSTPLLAS
                     SFLLLAFFLASCFSLTSGIFAVLL"
     assembly_gap    12643..12742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12743..13977)
                     /locus_tag="PLU11_02105"
     CDS             complement(<12743..13977)
                     /locus_tag="PLU11_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57928.1"
                     /translation="MRIALPVFTLSFVFCSMSPVKAQQADRFAWAITDAQPGGSNWSF
                     LRKLNLETGEYSAVLLDGNNSNLAVYNAGNKKPIETPLTDARFGNTINAAFGTGVAAM
                     AFDKKTNRLYYTPMLFDQLRYIDLKTMKVYFVMDKGFTGKPVKNADQGNIVTRMVIAA
                     DGFGYALTNDATQLIRFSTGKKFLSEDLGAIVDDPANKGVSIHNSCTSFGGDMIADNE
                     GNLFVISARNQVFKINPSNKVATHLGMINGLPEGYTVNGAAVNAENRIIVSSAMRTET
                     FTVDHKNWSATPYTISSTAWNSSDLANSNLLTSSEKNNNTVDFVSRNPAPNSGEGKIS
                     LYPNPVTNNQFVMQFSQLKAGNYSIEVTDVMGRRVLQQNMSISGDNHAQVMKLDPAAA
                     RGIYLIKVMDGGKQEVYST"
     gene            complement(14129..15910)
                     /locus_tag="PLU11_02110"
     CDS             complement(14129..15910)
                     /locus_tag="PLU11_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57929.1"
                     /translation="MKTFTQVKGLLTFVVFALLFNKNAAAQPAVTLNPASTQNVTAGA
                     TINFTAQRNSNSQNWTGGNSQFTFTWSSSPAGVTFTNNPNTVNGTSSSTVASFPTTGT
                     YQISCLVQEGGGGLNATSVATTVNVSTPVPANIWATSSNGTQISGFAVSNGTYQSGPN
                     NIFAPTFPGGTTGGSSTAALGRSAFPSLNLGHFYWLPNTAGNNGVVEIFAATSTGANV
                     TRVASFDVNGAGNNTSLGFVRLGMGPDGTGWILAGDGTTLFLAKFISNGVNPVTVTIE
                     DASVNLVGGAVSTFQNGDLCISGSGTIYALANDGNGVTQIFIGAPTGANTTLTKRWDL
                     VTPGGGNFSGTVNGVAFDVLGSIYISTGAGLYYINQNTANGPAGTVQCSLVVAMTGLQ
                     DLASNVFPAQSTLPVTLLNFSAAYRNGVTVLNWETENEVNFSHYEVERKTESGADYII
                     VDSKQANGNAGRSAYTSSDNISSLTDGVVYYRLRMVDVDGKFKYSNVIMVRKEKKTIT
                     GISLSPNPVISTANATVRFEAASNAVVSLRVMDMAGRQVLAQQNNVTGGTNSIQVNNL
                     NRLQPGLYIIQLVNGNELSSFKFSVVQ"
     gene            complement(16186..16779)
                     /locus_tag="PLU11_02115"
     CDS             complement(16186..16779)
                     /locus_tag="PLU11_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HPN57930.1"
                     /translation="MFYAYKKIRPVAHKSAYVHPQAVVTGDVIIGRNCYIGPGAALRG
                     DWGRIILEDGCNVQENCTIHMFPGITVILKEGAHIGHGAIIHGAQIGKNCLVGMNSVI
                     MDHVELGDESIVGALTLIKEGELIPPRSVVVGNPGKVIKPVTDEMLKWKTEGTELYQH
                     LPTDCFESLKPCKPRRKPTAESKRELPGYQSWKKSQP"
     gene            complement(16825..17262)
                     /gene="paaI"
                     /locus_tag="PLU11_02120"
     CDS             complement(16825..17262)
                     /gene="paaI"
                     /locus_tag="PLU11_02120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HPN57931.1"
                     /translation="MHTEKDKEALAVVNHMMEHDAFSRWLGISILEIREGYSRIRMTI
                     RPEMVNGFGIIHGGIAFSMADSAFAFACNNRNTLSVALDTSINFIKPVHIGDILTAVA
                     TELHNGKSTGLYQVEIRNQKDHVVALFKGLCYRTDKKLVPGND"
     gene            complement(17266..18039)
                     /locus_tag="PLU11_02125"
     CDS             complement(17266..18039)
                     /locus_tag="PLU11_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPN57932.1"
                     /translation="MSSILFERKGPIVFITLNRPDKFNAFNREMALLLQEKLDACKDP
                     GIRAVLITGAGKAFCAGQDLAEVTDPNGPGMARILSEHFNPIVTRIRELEKPVIAAVN
                     GVAAGAGANLAICCDIVVAARSAVFIQAFSKIGLVPDTGGSFFLPRLIGWQKASAIMM
                     LGDKIAAEEAERIGMIYKFFEDTGFAVSSQQIAETLATMPTRALHLIKKELQWSSTHT
                     LYEQLQNEDKLQQRAAQTADFKEGVNAFLEKRVPVFKGE"
     gene            18151..18444
                     /locus_tag="PLU11_02130"
     CDS             18151..18444
                     /locus_tag="PLU11_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57933.1"
                     /translation="MKLLIAVLLLSIPFLSCGPGKAYPDKVKQNFINSCAAKANGNTA
                     LCECLFEKVRERYTYAEFITLENDMKKGIQSQAFMNYMDSATRSCIIEKRGKP"
     gene            18486..19697
                     /locus_tag="PLU11_02135"
     CDS             18486..19697
                     /locus_tag="PLU11_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57934.1"
                     /translation="MFRFGLVLSFLLPLFAVAQPSEAEIRKQITNAGTKQIKFTKTTG
                     TRQWNSDAGNWEWVRGVEVIRTSDYAGIDLVVTGDVVYQYTGAGKYTYWKFRTLSNQY
                     LGIPNPTNKEIMDFLGTDWPKFYGYYFRKIVKLHGDPVLAENPDWTWHTPNSVSFKMK
                     QSFDFIYSNTEVQTMETIWNIRLYRDSPKDKWKNFISVKSQDAREEKITGSQKYTMEQ
                     VNDLEKKTLFFTFAEQKAKQEAAALPSVSLPEFKNAEEMVKYLHDILRNGNPEKFRAV
                     LLQLLHPGYFVEGSTVRLQPQQEQTLQQVITAVYNNKATYKMMYCQTPVYKVDQWGGG
                     SQKKTIYITGAVNNCNSSFTIAPVNMGYKEGVAQTALKITEYGIYVRQDQDAINFVSS
                     FSDRKKLCPND"
     gene            complement(19694..20185)
                     /gene="paaJ"
                     /locus_tag="PLU11_02140"
     CDS             complement(19694..20185)
                     /gene="paaJ"
                     /locus_tag="PLU11_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HPN57935.1"
                     /translation="MTPDIQKTWSYLEEIKDPEIPVLSIVDLGIVREVAIDDEAVTVT
                     ITPTYTGCPAMDMIAASIKMELMARGFAEVLVKFVISPAWTTDWMTEKGKLQLKEYGI
                     APPNPRQQVCNDKLFAPDEAVQCPHCESWHTRRISEFGSTACKALYQCEDCKEPFDYF
                     KCH"
     gene            complement(20202..20930)
                     /gene="paaC"
                     /locus_tag="PLU11_02145"
     CDS             complement(20202..20930)
                     /gene="paaC"
                     /locus_tag="PLU11_02145"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HPN57936.1"
                     /translation="MNSIINYTLYLADNALILGQRNSAWCGHGPVLEQDIAITNISLD
                     LIGQARSFYQYAAELTGNGATEDSLAYLRTEREFKNVLLTEQPNGDWAQTILRQFFFS
                     TYQFYLYQALVNQPDTRIAAIAEKSLKEVSYHLRWSSEWVIRLGDGTEESNRRLHKAL
                     EALWCYTGELFMPAEFEAGIDLVGLKNDWSTKVKTILEEATLAVPDNVFMHSGGKTGN
                     HTEQMGFILTELQYMQRAYPDSEW"
BASE COUNT         5459 a         4911 c         4768 g         5831 t
ORIGIN      
        1 gccacattcg ctttccggcg cagccagttg tgcttatcag cgggtaatcc atcttccaca
       61 tagagaaaaa cagattgatt cagccggctg atctccacag caatcatctg gttccggctc
      121 ttcgccaatt caacaatttt caaccccata tccatggcga tggcattgga gaatgtatcg
      181 agttcgattt tgaggaggac ttccttttca ttgctttcag cggacatata caggcgtttt
      241 cggatgagga aatttaaaat gatcgtgtaa agatagcaag cagcgactta ttcaggtgat
      301 gggatcctca tttaccggtc atctgttcca tccagctcat gatcagcgct ccctcttccc
      361 ctgagcaggg gtttgaggcg ttgccggaaa aatagttgac gacttcagcg atcatgggct
      421 gttgtacatg tgctaatggg tcaaaggtaa attcagttgt ttgaccgtca agggtcagcc
      481 gtatctcttg cttgctgaaa aaactgaaat ggatggtacc ggtactgccg ataatctcgc
      541 aatcgtcact ggcttcagct gctgtaaaac accaggtgcc gctgaataaa acaccattgg
      601 taaaacggat cataccggtg acaatatcat cggcggcata gtggccgccc tggttgctgg
      661 ccatgccggc tattttttcc gcatcaccga gtaaatagta cagaatatcc agctggtggg
      721 gggccagatc atgaaagagt ccgccaccag agatagccgg attgacccgc caggcatttt
      781 ttgtatcagt cagttcagcg gcggcaggcg cctgcctggt aaactgtaaa cggacgaacc
      841 ggatttcacc gatcacttgt tgtgcaagca ggctctttag ttttaagaac aggggccatt
      901 gccggcggta atgggccacc gttaatttta taccggccgc tgcagccacc gccgccatgt
      961 ttttggccgc agcataattc agggccatgg gcttttccac gtatacaggc ttgcctgctt
     1021 taatggcggc caatgtatat ttttcatgcg catcgggtgg tgtggcaata tagatggcat
     1081 tcaccccggg atcctgtatc aatgcatccg catctgcata ccaccggtcc acctggtgcc
     1141 gcttcgcata gtcggctgct ttggtggtat cccggcgcat cacggcaatc agccttgagc
     1201 ctggcacttt attaaatgcc ggcccgcttt taacttcggt tacatcccca cagccaatga
     1261 taccccagtt gatcatgaat tactccgtca ggattttgtt gatggataaa aataactcat
     1321 tcgggtgtaa aatggcgttc gttttactta ctccttaaag ggcttgtatt ccttgctata
     1381 gttcgatgct cagtctcacc ttacccttca atccttgttc tacaatttta tgcagggtgg
     1441 aaagccgtac ttctttgata ttattttcaa ttcttgagat ataggatttg gtagtgcctg
     1501 ttttttcagc cagttcctgt tgggtcatac ctttttcaac gcgtgccatt tgcagcagtg
     1561 cgccaatttt gaaattttca taaccagctt caaatttatc ccgtttgggg gtaccttttt
     1621 ttccatagtt tttttctatc agctggtcta aggttatgag attatttttt ttcgttttca
     1681 tattcctgtt taatgcttaa agctttttgt atttctgatg atggagtttt tttggttttc
     1741 ttattaaatc catttataag aataattaat tgtccctcat caaaaaagca aaaaatcctg
     1801 aaaatatcag atcccaattg tacccgcacc tcatacagtc ctttactgtt ttcaatatgt
     1861 tttaaatagg tttccgggat gactggtaac gtttcaatca gcttaagggt ccagacgatt
     1921 ttagctttta ctttttcacg ttgttgttca tagaataatt caaaataatc tttaaagaaa
     1981 acaatagtcc tgaatttttg ggtattggtc ataacaaaga tagtaaaagt tgcactttag
     2041 tgcaacaatg attttagtct tttacagctt taattttcca gctctattat attggtaagc
     2101 tccaatctat taacttgaaa atcaaaacta aaggctggaa aagtacctgc cttgataaaa
     2161 tcaacacgga atcctgttat ttttccccgg aataattggg gtaattcgga aaggatataa
     2221 ggtttccatt atgtagataa taagactgga tttttaccca ttccacacca gccggccttc
     2281 aattttatac aactttcggc aaggtgtcat ttccttttta ctgaatttct tgtattggta
     2341 attgattttg cgattgctag tataaaagtt ccttgctttt cgctggtact catttttatt
     2401 cttatcgtta aataaatagg tataaaagtt ccagcaatca ttttcaataa gatcgtattg
     2461 agtctgtgta atcagaaaag caggaaagtc cgccagttct tcctgtgcaa aatagctgac
     2521 ttctcctaca ttcagttttc cttttttaca gttgttgtat atagtggtag ggtgggtatc
     2581 tgtaaggcac tccagaagta cagggatcat tttttctcct tgctggatta ttttccaata
     2641 ggcttctgca ctgtagtcca gcttttcagt tttcatttcg cttaaagcgg caatcaggct
     2701 gtctttttgc cgggtaatgc cgaggtgact gtacgtgtcg gttcctttga cagggatttc
     2761 gccggaatcg gttaattcat tgttattaca actgatactt aagataatta ttagtaatac
     2821 aattagcctt tttttcatgg ataagccagg tatatttttc ttttttactg ctgagtgaaa
     2881 tgatattgca tttaattaaa aaagatatca accttgttta taatttttca caaaataata
     2941 gcccagtaaa gggataaaca aggtcaggta agtaagagtc ttccgcctgg atttactcag
     3001 ttgtaatttg cccaaacgat gaaaactcag atacataatt atgactgaga gtaaaatggc
     3061 agctgataag gagattgggc tggtcatagt gctgtgttaa taaggtttac ggttagtcgg
     3121 ttcgaacgcc ggttaatgct cttgtaaggt acatgaaatc cgggcttttt caatcaccag
     3181 taaaatagct acttcggaat agccaatact ccaagccagc ctttgcaatt tcagtcccaa
     3241 agccgctcct gattagtgtt tttcttttgc cggcaacggg taccaggaac cgggtgatga
     3301 tccggttttc ggatttcagg aaaatagtct caatacgaca ataagattcc agaaccatcc
     3361 catctttttt cgcctttttt tgtaatcgac gggctatccg ctgcatttga cgggttaccc
     3421 cgcatcccat ccccatttga tatacacgcg gatacaggct gtcaatccgg aatgccggcg
     3481 atgcaagcag gctgctatcc aaatacacag agctataaag tttatcggca ggatgattat
     3541 tcaaataagc cttgctctcc catgaattgc caaaaggcgt aaagtgatac tcttcaaaga
     3601 tgccgtactt cccggttttg aaatggtaac cttcaatgag gacaatatta attggggcca
     3661 gttcccggaa acgttgcatc aggctggccg tatctctttt gctgataagg gtgagaaaga
     3721 gctggtactg tgtggtggta aggccgtctt tgacagccag caaatgtttc tttgtttggc
     3781 tattaagatc ttctgctatt tgtgcggaac tgtttaaacc gaatagtagc agtattaata
     3841 atgcgagctt catgaagtca gctaacttag aaattattct gatgcagaaa tgttaaaata
     3901 tcgattgttt ttaaagagcg caagccgaac caatcctggc atacttactt aaaaaggtga
     3961 cccctttttt actgtcattt tcaatagtac tgtcggaaaa cggcttttcg gtaataagat
     4021 aagaaaaggc acaaatatct cctgccgcaa agtcaagttg aatgtaatcg ccttgtgtac
     4081 ttattgacag acagtttgtt tttttcagca tcttttttaa agttatgaca gtatttgttg
     4141 tccagtcaag cgttttcaaa aacgatctca tctgttctga atccattcgc atgtccctcc
     4201 cggcgttata cgggtaatca gggctaatgg cccaatatgg ctgggcgatt gaaatggtat
     4261 acctgttttt tgaaggctta aagaaaactt cgttgctatc tggcttcaat ttcaaaaagt
     4321 atgcagcaag ctccatgatt tgcttactgt ttttttcata gttgtttata gtatcatcac
     4381 gggtaatatg tttgttaaca caagacgttt caacaatgaa taaaattgtt aaaaggactg
     4441 ctatatgaaa taagcggttc atttataaag ataatcgcag atggagagta agtctgacgt
     4501 attccaatcc aacgcaatgc ttaggtcgcc ggccggttcc ccactgttcc cgagttccgt
     4561 aaaacaggac tgcaaagcct tttagaggtt aaagtacttt gtttatttgt tcttctaagg
     4621 cttattttgc caccaccttc tcccacttcc ccaaccactc ttccgaaatc gtcgcaccaa
     4681 gaccaatcgc ttccggtact tccaccacgc cattggcttt atacacgata cctccggtga
     4741 ccggatcttc ccgcagcatc aatgcggtat caaaatcaaa gtgtacaata tttggggagg
     4801 agagggcgaa atgcgcaaaa gcagtcatgg ctaaccgtga ctcaatcata gcgccgacct
     4861 gcaggtgtaa tccggccgct tcagccatcc gggccatttt aagggccttg aaaatgccgc
     4921 cggatttgcc gagcttgata ttgaaataat cacaggcacc cagtgcaatc aggcgttcgg
     4981 catcatggtc atcgccgcaa cattcatcgg ccataatggg gatgggactt tctttcttta
     5041 cacgcggcaa agagagataa ttccagcggg caatgggctc ttcgcagtgc tggatatcaa
     5101 ggggagctaa tgcgtttaat gtctcaatgg cttcttccac accccaaccc tggttggcat
     5161 cgatgcgtaa aggaatatca ttgccaactg cggcacgaat tgctttcata cgtaccacgt
     5221 ctttctttcc atccttaccg agtttcactt tgatcgccgg atagccctgg gcaaggatct
     5281 ttatcgcctg ggccgccatg acatcgggct cagcaagact aacggtataa tcggttatga
     5341 tcgtcttgtt atttttaccg ccgataaatt gataaagcgg aaccccagca ttttgactgg
     5401 cgatatcgta taaagctata tcaaacgcac tctttatact gccattggca tagatgatgc
     5461 tgtccatcag gttgacagcc ccttcaatat cgagggcatt gtggccaatc agggcgttgg
     5521 caaatagttc accaacgacc atacaggtat cctggctctc accattgatg ggcatatacg
     5581 gactgcattc cccaaaacca acaatccctt ccttagtaag tacccggatc agcacattgt
     5641 cggctgtata atcggtgcca agagaagtga taaaaggctc gatcagggga atggacagct
     5701 tgtacagttc gatctgttgt atagtgatag tgttactcat aacagttgat taaaataaga
     5761 tccgtagtcc gccgcagagc agtgtcgtaa agggcgcatt atcgttgact aataaaaacg
     5821 gactcgggta accgggtgag ggcactgtta cccgggcttc ggtatagaaa taaaaatcgg
     5881 cacccaacgg tttttcccaa cccaaggcca ggtccagggt catccgttta aaattggccg
     5941 taccttcccg tacgggtatc tggtaaaggg ccgtatcgat cgtacggtta tgtgaatttt
     6001 ccacccttcc catcagtaag ccaaaaccgg ccttgccgta aagactccac ccggtttcca
     6061 gttggggatt gccatgcagg taatgcttcc agcctaccga taataaacgg aatcggagct
     6121 taccggcatt cgtatactgt atttgttgcg gcagggtaac tgcatccgtg gccttcgccc
     6181 ggagtacgtt attgtattta ccaaaactgc aataactgaa gttcacctgt attccgtctt
     6241 tctccgccag gtgaaaatcc agctgaaccg aatgattcag cccggtaaac tgctgcccct
     6301 tcggccaatt gcgtatcgcc cccaggtcgg taccgataga aaaagaaggg tactgggcca
     6361 gtgtactttt tcccaagaaa gtaagcaggg ttaatatgat gagttggtgg cgcaagaaag
     6421 tgttatgcaa tgttactaaa ttattcagaa gagcccccta aatcccccga agggggactt
     6481 taaagactca aattatttga tcaatcttat ttaatcgtct tgcgtaaagt ttcccgcaaa
     6541 gccattaagc ctttccgaac aaagggaaag tcttttaagc ttagcgggat acgatacgca
     6601 attctgtgaa agaaagagat tgcgaacttt cagtgtctaa agtccccctt cgggggattt
     6661 agggggcctt cttcaaaaaa aagcgggtga ccgttttcag ccacccgccc atacttaaaa
     6721 cttataactt aaaacttaca actcctccct atttcatcag cttcagttcc ttaataatat
     6781 tcgcgccacc gcccagctta tcaatgcacc acaacacata gcggatatcc acacagatcg
     6841 tgcggttcag gtctttgtcg aaggcgagtt tgtggctcag cgcttcataa ttgccatcga
     6901 aggccagacc gatcagttca ccacggccat tgatcacggg agaaccggag ttaccgccag
     6961 tgatatcatt ggtagtgatg aatccgatca ccaggtcctt gcgggtggga tccatgtact
     7021 gaccgaaatc ttttttcttc agcaactcga tctgattggc cgggaggtca aattcataat
     7081 caccggcctt gtatttttcc agtaatcctt ttgatgttgt gacatagtca tagaaaaccg
     7141 catcgcgcgg acgataggat tttacattgc catagctcac ccgcatggtg aaggtggcat
     7201 cgggatacat catcttcgct ttggcgggat ccatttccat aacacctttc aggtacaagc
     7261 ggccaaactc ggcattcttg gtgttgaatt gttgtgagaa cggcgcatac ttggtattat
     7321 aattcaccag gaaagcactg gcatgggcaa aagccaggtc actttgcagc gtaacggcat
     7381 ccggattggc cacaaaagcc gcccatttcg catcgtcgaa gatcatggtc ttgctgaata
     7441 catcagcggc atatttttta taggtcgatt cgtcattcag cgcaccataa ctgtttttca
     7501 gcgtgccata aaaaccaagc ggatgttgct gtttgtctac atcctcatag aacatacggg
     7561 ttacggcagc cagtatattt tcatccgaag ttttgttttc atctttcagg aaattggtgc
     7621 gctggttatt ggctgcttcc acggctttgg caatatcggc tttggaagaa ccggtcttta
     7681 ccattgccgc ttccacctgc atcagggaac cggcaaaagc gatcagcggg gaacccatca
     7741 cgccttctac aatatacaca cgatgcttgc tgtaaggtct ccaggcatca taggctttac
     7801 cccagtcggc aaacaggttt tcatattcag gttttccttt agcccaggca ttgaactttg
     7861 tttcagctgt ctgtttttgc ccgaacacat cgtatttcag taactgcttg gtttcgccat
     7921 caaagaattt ccaatagttg gcgatgcctg cataggagga agcgagctgc agtttcaccg
     7981 cagggtcttt cttcatttcc tcaaacatat atttcaacct tgcatcccgg agttttacca
     8041 gggtcgggtt gttgatatct gtagcgatct tgataccata tgaactttcg taacggttgg
     8101 taccaccggg ataaccccag atcatggaga agtcgccatc tttcaacgga cgtaaagaaa
     8161 cgggcaggta ccatttgggt ttcatgggtt tgttgccttc cgcaaatttg gcgggtttgc
     8221 catcggcgtc ggcataaaca cggaacacgg agaagtcgcc ggtatgacgg ggccattccc
     8281 agttgtcagt atcaccaccg aatttaccta cactctcggg cggggtgcct acgagccgga
     8341 tatcggagta acgctgataa acgaaggcga ggaattggtt gcctttgaaa agcgggctga
     8401 cacgggtctc gataaactga ccggcatcac tcattctttt attgatggca gccagtacag
     8461 cgttttgttt cgccacgcgt tctgtcccgc tgagtccctg cagggagtcc atcacttctt
     8521 tggttacatc atcaatgcgc aggagaaact gcacactgag ggtggtgggg atttcttcac
     8581 ctttgttacg ggcataaaaa ccattctgga ggtaattatg ttccatcgta ctggccgaag
     8641 caatagcatt gtatccgcag tggtggttgg taaaaatcag tccttcggca cttacgatct
     8701 cgcccgtaca gccaccacca aagatgataa tcgcgtcttt cagcgatgct ttgttgatgt
     8761 tgtaaagctg atccttggtg agtttaagac cttttttcac catatcatta taaacctgct
     8821 gtcctaataa aagtggcagc cacattcctt catcggccat ggatttcagg gccagggaaa
     8881 gaaatagtac aatcgtcagg aaaatcttct tcatacatgt gattttaggg ggcaaaccta
     8941 ctgaaattcg gttttatggg acctgacttt catctgaatc gagtattttg gggggcaatc
     9001 actgcttatg caaaaaaatc aactcgctgc ggtcatactg gccgcccttt tcctggttta
     9061 ttcctgcggt aaaaaaggta atacgcccaa tccgcccccc aatccggtgc caggttgttc
     9121 tagcggcaat gccccggcca atggatcctt tgttaccagt accagtgtca ccctcagttg
     9181 gaattccgta ggtacggcca gtgcctatga catatacctc ggcaccacgg ctgtccccac
     9241 taccctgatc gccgccaatg taacgggtac cagtaccact tatacgatac ccgccacacc
     9301 caataccacc tattactggt atgcactgcc aaaaaacacc ggaggttctg ccaccggatg
     9361 taccggcacg gtgcagtcct tcacttatat cgtgattacg ccgccggcct cccttggtta
     9421 ttatgtagtg ggttatatgc ccagctaccg ggtgttggcg gatattccgg atgtgaaatt
     9481 ccggatgacc aatgtggtgg tctatgcgtt ttacggggtc aatggatcgg gtaccatttc
     9541 agcacccaca tccggtagca cactggcggc tacggttacc aaggcccggg ccaataatgc
     9601 gaagatcctg ctgggtatca atgatggcac aggtgatggc aagaccaatt ttaaaaacat
     9661 ggcttccacc gcggcaggcc gcaccaattt tattaaaagc gtgatggccg tcgtccgcgc
     9721 caataacctg gatggggttg atatggactg ggaattccca agtacctccg atggtacgga
     9781 tgtcacgttt acggcattaa tgaaagaatt atcggataca cttcaccggg atgcccgtta
     9841 ttatttatcc gccgccgtta cagccggaaa atatgcggga ggtatccggg atgccattaa
     9901 aaatgaaatc ttcccttacg tggatttctt taatatcatg gcctatgatg atttcagtac
     9961 cagcacgcct taccgccatc acagtgatta taccctggct accgtctgtc tcaattactg
    10021 gctcactacc cgtggtatgc cggccgctaa agcggtactg ggtattccgg cctatggccg
    10081 tccttcgggt atgacacaga gcggcactat caaaacctat aaagatattc ttgccgctgg
    10141 cggtaatccg caatacgatt cagccacggt atccaccaca acttttcctt cgtacaccgt
    10201 ctactacaac ggacaataca ccaccaagcg caaagccaaa ctggccaagg atattgcggc
    10261 cggtgtgatg ttttgggaga aaggacaaga tgcgatggat ggaaattcgt tgctgaaggc
    10321 ggcttgtgac acaattggga ggacttatta gtttgattgg tgatttgttt tgaagaacca
    10381 agatgcccct ctttcgctaa agctacagag ggcaaagcaa gatccaagat gccaaataag
    10441 caccaaaaca aatcccaaaa ccttctaagg aggttttggg atttgttatt tggaatttct
    10501 ttggcttctt ggtgtcttgt atcttgatgc ttttcttact tgcatcctgt ccaggttctc
    10561 agttcctgat taaaaaacca atacattcca ttgcaataac tgaagttgaa catttcgtaa
    10621 gtggcagtgc cttccggcag ttttaattta ctgacgagat agccgttgga aacatcgtat
    10681 aattccactt cctgggtttc aatgtttagc agcccgattt cactgccctt gatacccgta
    10741 taaatgatgg aggcactgtt atactcttca ataacgtcgt aattatcggt caagccggta
    10801 tcgtctttct tagttacgcc caattgcaga ttaatggttt tgatataggc cgtgtccttt
    10861 acgctatact gttccaggtt gccgtcttca tccagaaaat agagcagatt ttttgcagga
    10921 tcataagcgc cgaccgattg ttcggtgggc tgcagctgtc cctcatggaa agtgatgatc
    10981 tctccgggct taccaccttc aaacaaattg tattgccccc agccataatc gctatagcca
    11041 tttgcctgca gggccttggc gaaggggttg taccataagc cgcggacatc atattgcgtg
    11101 gtgataccgt cttcaaaaac tttcttgcct tgggcatcaa agacaaccaa agggaattcg
    11161 gaattgccag ccatggcggc ataatatttt ttctgaatgg ggtgccaggc aacagctgcg
    11221 ccattggatc cgccttcttc agtaatttct aacgtaataa cttttttcag ggtttttaca
    11281 gcttgcgcat tcgactggaa gctgagcagc aacgtaaggg ctgcaagaat cagagccttg
    11341 ttcatgggta attatttgcc ctaaagctag actaatctgt tgccctttta catacccgga
    11401 agacggtatt attctacggg ttcattcagg atcttccaga gcatatcttt cagctcggtt
    11461 aatcctttat tggtaataga agaaataaag acatggggta catccgcagg caattctttt
    11521 tcaatggcct gtttgagttc atcatccagc atatcacttt tactgaccgc aatgatgaat
    11581 tgtttatgga ggagttcggg attgtattgt tctaactcat tgaccaggat gtcatattct
    11641 tttttgtggt catcacaatc tgcgggaata aggaacagca acaccgagtt tcgctcaata
    11701 tgccggagga agcggtgccc cagtccttta ccttcagcgg caccttcgat aataccggga
    11761 agatcggcaa tacagaaact tttcccgtcg cggtattcca ccatacccag gttgggtacc
    11821 agggtggtaa atgcgtagtt ggcgatcttg ggtttggcag ctgtgattac agagagcagg
    11881 gttgatttac ccgcattcgg gaaaccgacc agtcccacat cagccaacac cttcaattcc
    11941 agtactttcc agccttctac accggggagt cccggctgtg cgtgttcagg tgcctggttg
    12001 gtaggggtgg caaaatggga gttgcccaat ccgccttttc cgccgggtaa ccagatcact
    12061 tcctgtccat cttcgaggat ctcgacttcc tggccaccgg attcttcatc ccgggcaatc
    12121 gtccccagag gtacatcaat tatgatatct tttccgtccc ggccatggca gttgttggca
    12181 ccgccgcgtt cgccgtcttc ggccagtacg tttttgtaat agcgtaaatg gagtaaggtc
    12241 cagagattgc tgttgcctct caggataata tgggctccgc ggccaccatc cccaccatcg
    12301 ggtccgccga gtgccgtgaa tttattacgc ataaagtgct tattaccagc acctccatgg
    12361 ccactgcggc aaaatattct gatctgatca acaaagttac tcttctccaa aacggtgttt
    12421 tttgtgaatc cggctttaca gcaggactgc aaatatacct gaagtaagcg agaagcaaga
    12481 agcgagaaag aaagcgagaa gcaagaagct agaagcgaga aggggtgtac ttagtggatg
    12541 ttgctggtaa gaacgacttt ttccttcatc ccgtaaacct tcaactacaa gaatacaatc
    12601 ccgcagatgg cgcagatgga accgcagata gacgcagatt tcnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnttagtgga atacacttcc tgctttccac catccatcac
    12781 tttaatcaga tagatccctc tcgctgcagc cggatccagt ttcatcacct gtgcatgatt
    12841 gtcgccgctg atactcatat tctgttgaag cacccgtctg cccattacat cggttacttc
    12901 tattgagtag ttaccggctt tgagttgact gaactgcatc acaaactggt tattggtaac
    12961 cgggttgggg tagagactga ttttgccctc tccgctgttg ggagcagggt tacgactgac
    13021 aaaatctacc gtattattat ttttttcgga actggtcagc agattgctgt tggccaggtc
    13081 agaactgttc caggctgtgc tgctgattgt atatggagtg gctgaccagt ttttatggtc
    13141 caccgtaaac gtttcagtcc gcatggcact gctgacaatg atacggtttt ctgcattcac
    13201 ggctgcgcca tttacggtat agccttccgg caatccgttg atcatgccaa gatgagtggc
    13261 caccttattg gatggattga ttttaaatac ctggttgcgt gcagaaataa caaagagatt
    13321 gccttcatta tcagcaatca tatccccgcc aaaactggta caggaattat gaatagacac
    13381 tcctttattg gcgggatcat ctacaatggc cccgaggtcc tcgctgagaa attttttgcc
    13441 ggtactgaaa cggatcagct gggtggcatc attggtcagg gcatagccaa atccatctgc
    13501 ggcaatcacc atccgggtaa cgatattgcc ctgatcggca tttttaacag gcttaccggt
    13561 aaagccttta tccatgacaa aataaacctt catggtcttc aggtcaatat agcggagctg
    13621 atcgaaaagc atcggggtat aataaaggcg gtttgttttt ttatcaaaag ccatcgccgc
    13681 cacaccagta ccaaatgcgg cattgatcgt gttgccgaaa cgggcatcgg tcagtggcgt
    13741 ttcaatgggt tttttgttac cggcattata cacagccaga ttgctgttgt tgccgtccag
    13801 tagtacggca ctgtattcgc cggtctccag gttcagttta cgcaggaaac tccagttgct
    13861 gccacccggc tgcgcatctg tgatggccca ggcaaagcgg tcggcttgtt gggccttaac
    13921 gggggacata ctgcaaaaaa cgaaggacag cgtgaaaacg ggtaaagcta ttctcatatt
    13981 cagggttttt gttgtcatta aattacaaca tttccccagc cgtcaaaaac aattttttat
    14041 aagtggtata aaaaataaac ccctcccgga cgaaccaagg aggggtcaag gtataaaaac
    14101 cctgagcatt gcctttctga tcaattaatt actgtaccac agagaattta aaagaggaca
    14161 gttcattgcc attcaccagt tgaataatat agagaccggg ttgcagcctg ttcaggttat
    14221 tgacctggat gctgttagtt ccaccggtta cattgttttg ttgagccagt acctgacggc
    14281 cggccatatc cataacacgc aggcttacta cggcattgct ggcagcctca aagcgaacag
    14341 ttgcgttagc tgtgctgatc accgggttag gactgagact gatacctgtg atggtcttct
    14401 tctccttgcg caccatgatc acattgctgt atttaaattt accatccaca tccaccatcc
    14461 ggagacgata gtataccacg ccatcggtca ggctgctgat attatcactg gatgtatagg
    14521 cagagcggcc ggcatttcca ttggcttgtt tgctgtcaac gatgatataa tcagcaccgc
    14581 tttctgtttt tctttctact tcataatgac tgaagttgac ttcgttctct gtttcccagt
    14641 tcaggacagt aacgccattc cggtaagccg cgctgaagtt cagaagggtt acaggcaggg
    14701 ttgattgagc agggaatacg ttagaagcga gatcctgtaa accggtcata gctacaacca
    14761 gtgaacattg tactgtaccg gcaggtccgt tagcggtgtt ctggttgata taataaagtc
    14821 cggcaccggt agagatataa attgaaccca atacatcaaa agccacaccg tttactgtac
    14881 cggaaaaatt accaccacca ggtgttacga ggtcccaacg cttggtcagc gtggtattgg
    14941 caccggtagg agcaccgata aagatctgcg ttacgccatt gccgtcattg gccagtgcat
    15001 agatggtacc tgatccggag atacaaagat caccgttctg gaaagtagat accgcaccgc
    15061 ctacgagatt cacactggcg tcttcgatgg taacggtaac aggatttaca ccatttgaaa
    15121 taaacttggc gaggaacagg gtggtgccat caccggcaag aatccatccg gtgccatcag
    15181 gtcccatacc gagacgtaca aaaccaagac ttgtattgtt gccagctcca ttcacatcga
    15241 aggaagctac acgggtgaca ttcgcaccgg tagaagtggc ggcaaaaatt tcaaccactc
    15301 cattgttacc tgccgtgttg ggtaaccagt agaaatgacc caggttaaga ctggggaatg
    15361 cactgcggcc aagggcggca gttgaagaac caccagtagt accaccgggg aaggtaggtg
    15421 cgaaaatatt gttgggaccg ctctggtatg tgccattgga aacggcaaat ccggagatct
    15481 gggttccatt ggaagaagta gcccagatat tagcgggcac cggtgtggat acatttacag
    15541 tagtagcaac agaagtggca tttaaaccac caccaccttc ctgaaccaag caggaaatct
    15601 ggtatgtgcc ggttgtgggg aaagaagcaa ccgttgaaga gcttgtacca ttcaccgtat
    15661 tggggttatt ggtaaaagtc acaccagccg gacttgaact ccaggtaaat gtgaactggc
    15721 tgttccctcc ggtccagttc tgactgttgc tgttacgctg agccgtaaaa ttgattgtgg
    15781 ctccggcagt aacattctgt gttgaagcgg gattaagtgt tacggcaggc tgtgctgcgg
    15841 cgtttttgtt aaaaaggagt gcgaatacga caaacgttaa caaccccttt acttgtgtaa
    15901 aagttttcat gctcatggtt ttatgtagaa aaaataataa taccttgttc aaatggtttt
    15961 gaacggaaat ggaccaatgc tcagggtcat ctacaggaat ggagttaagg gggtcttaat
    16021 aaggagacat ggacggatta cactaacaaa aaaaacgatc taatgcttca gggttttttt
    16081 agaggaattg tgattcgcag aagacaaaag taatatacgg atgcagattc tgcaagtaaa
    16141 atcgcattta ttttttggga acctactcaa ctatatatcg tgtttttacg gctgactttt
    16201 tttccagctc tgataaccag gcagttctct ctttgattcc gccgtcggtt tacgtcttgg
    16261 cttgcagggt ttcagacttt caaaacagtc ggtcggtaag tgctggtaaa gttccgtacc
    16321 ctccgttttc cacttcagca tctcatcagt taccggtttg atgacttttc cgggattgcc
    16381 caccaccaca ctcctgggtg gaataagttc tccttctttg atcagggtca gggccccgac
    16441 aatagattca tctccgagtt ccacatggtc catgatcaca ctgttcattc ccaccaggca
    16501 attcttaccg atctgtgcac catgtataat ggccccgtga ccaatatgcg caccttcttt
    16561 taatataacc gtgataccag gaaacatatg aatcgtgcag ttctcctgca cattacagcc
    16621 atcttcaaga atgatccgac cccagtcgcc acgaagcgcc gcacccggac cgatataaca
    16681 gttgcggccg atgatcacat cgccggtcac cacggcctgc ggatgcacat aggcggattt
    16741 gtgggccacc ggcctgattt ttttataagc gtagaacatg gggcatattt taatcggtaa
    16801 tcaacaggaa tgtataaacg aaaatcagtc gttgcctggc accagttttt tatcggtacg
    16861 gtaacaaagc cctttgaaca gggccaccac atggtctttc tggttgcgga tctccacctg
    16921 gtacaggccg gtggacttcc cgttatgaag ttccgtagcc acggccgtca gtatatcccc
    16981 gatatgtacg ggtttgatga aattgatgga agtgtccagt gccaccgaca aggtattgcg
    17041 gttattgcag gcaaaggcaa aggcactgtc ggccatggag aacgcgatac ctccgtgaat
    17101 gatgccgaat ccatttacca tttccgggcg gatcgtcata cggatacggc tatacccttc
    17161 ccggatctcc agtatggata tgcccagcca gcgactgaat gcatcatgct ccatcatatg
    17221 attcacaaca gccagggctt ctttgtcttt ttcggtatgc atagattatt ctcctttaaa
    17281 tacaggtact cttttttcca ggaaggcatt cacgccttct ttaaaatcgg cagtctgtgc
    17341 agcgcgttgc tgcagtttat cctcattctg caattgttca tacaaggtat gcgtggacga
    17401 ccattgcagt tcttttttaa tcaggtgcaa ggcccgggtg ggcatagtgg ccagggtttc
    17461 tgcgatttgc tgactactca cagcaaaacc ggtgtcttca aaaaatttat agatcatccc
    17521 gatccgttcg gcttcttctg ccgctatttt atcacccaac atcattattg ccgacgcttt
    17581 ctgccagccg ataagccggg gcagaaaaaa gctgccgccg gtatcaggca ccaatccgat
    17641 ttttgaaaaa gcctgtataa atacggcgga acgtgcggca acaacaatgt cacaacaaat
    17701 ggccaggttg gcaccggcac cggcagccac cccgtttacc gctgcaatca ctggtttctc
    17761 cagttcacgg atccgggtca caatcgggtt aaaatgttca ctcagtatcc tggccatccc
    17821 gggcccgttg ggatcggtta cttctgccag atcctgacca gcacagaatg cctttccggc
    17881 accggttatt agtacggccc ggatacccgg atccttacag gcatccagtt tttcctgtag
    17941 caacagggcc atttcccggt tgaaggcatt gaatttatcg ggccggttaa gggtgataaa
    18001 gacaatcggt cctttgcgct caaagaggat ggaagacata gctgaatttt aaagcaaatt
    18061 aggaaagatt cggctcaacc aagggcccgt tttcagcccg aacggaaatg tgacggattt
    18121 ctttatctta atggcaccaa accattaaac atgaaactac tcattgcagt cctcctgctg
    18181 tccatccctt tcctttcctg tggtccgggt aaagcgtatc cggataaagt aaaacaaaat
    18241 tttatcaata gctgcgcggc caaagccaat ggcaatacag ccctgtgcga gtgtttattt
    18301 gaaaaggtca gggaacgcta tacctatgct gaatttatta cccttgaaaa tgatatgaaa
    18361 aaagggatcc aatcacaggc gtttatgaat tatatggatt ccgccacacg gtcttgtata
    18421 attgaaaaac gaggaaagcc ttaaattgca aagccattaa acgcatacac ctaaaaaatt
    18481 acattatgtt caggttcggc cttgtccttt ctttcctgtt gcctttgttt gccgtcgctc
    18541 agccttcaga agctgagatc cggaagcaga tcacgaatgc aggcaccaaa caaatcaagt
    18601 ttaccaaaac taccggcacc cggcaatgga acagcgatgc ggggaactgg gaatgggttc
    18661 ggggtgtgga agtgatccgt acaagtgatt acgcagggat tgacctggtg gtaaccggtg
    18721 atgtggtata ccaatatacc ggcgcgggca agtacaccta ctggaaattc cggaccctga
    18781 gtaatcagta tctgggaata cccaacccca ccaataaaga aattatggat ttcctgggaa
    18841 cagactggcc taaattctat ggttattatt tccggaaaat tgtaaaactg catggcgatc
    18901 ccgtactggc agaaaatccc gactggacct ggcatacgcc taattcggtt tcatttaaaa
    18961 tgaaacaaag ctttgatttt atctattcca ataccgaagt acagaccatg gaaacgatct
    19021 ggaatatccg cttgtaccgt gattctccaa aggacaagtg gaagaacttt atatcagtaa
    19081 aaagtcagga tgcacgagag gaaaaaatca ccggttcaca gaagtatacc atggaacagg
    19141 taaacgacct ggaaaagaaa accctgttct tcacgtttgc agaacaaaag gcaaaacagg
    19201 aagcagcagc tcttccgtcc gtttcgctgc cggagtttaa aaatgcagag gaaatggtca
    19261 aatacctgca tgatattttg cggaacggca atccggaaaa gttcagggcc gtgttgctgc
    19321 agctccttca tcccggttat tttgtggaag gcagtacagt ccgtcttcag cctcaacagg
    19381 aacaaacctt acaacaggtc ataacagcgg tctataacaa caaagccact tataagatga
    19441 tgtactgcca gacgccggtt tataaagtgg accaatgggg cggaggcagc cagaaaaaaa
    19501 ccatttatat caccggtgcc gtgaataact gtaattcctc atttacgatt gctccggtca
    19561 atatgggcta caaagaaggg gtggcgcaaa cggccttaaa gatcactgaa tatggtatat
    19621 atgtccggca ggaccaggat gcgatcaatt ttgtgagttc attcagcgac agaaagaaat
    19681 tatgcccgaa tgattagtgg catttaaaat aatcaaaggg ttctttgcaa tcctcacatt
    19741 ggtaaagggc tttgcaggct gtactgccaa actcactgat gcggcgggta tgccagcttt
    19801 cgcaatgcgg acattgaacg gcttcatccg gtgcgaataa tttatcgtta caaacctgtt
    19861 gccggggatt gggtggtgca atgccgtact cctttaattg caatttccct ttttccgtca
    19921 tccagtcagt ggtccaggcc ggactgatca cgaactttac cagcacttcg gcaaatccgc
    19981 gggccatcag ttccatttta atcgaagccg caatcatatc catggccggg caaccggtat
    20041 aggtgggggt gatggttacg gtgacagctt catcatcgat agctacttcc cgtacaatac
    20101 ccaggtccac gatactgagt accggaattt cggggtcttt gatttcttca agataagacc
    20161 aggtcttttg aatatcggga gtcattgtat tcatttacac tttaccattc actgtctgga
    20221 taggcccgct gcatatattg aagttctgtc aatataaaac ccatttgctc ggtatgattg
    20281 cctgtcttac ccccgctatg cataaataca ttatccggaa cagccagcgt ggcttcttcg
    20341 agtatggtct ttactttggt actccagtcg tttttcagcc caacaaggtc aattcctgct
    20401 tcaaattcag ccggcataaa taattcaccg gtataacacc agagtgcttc cagcgctttg
    20461 tgcagccggc ggttactttc ttccgtgcca tcgcccaggc ggatcaccca ttcactgctc
    20521 cagcgcagat ggtaacttac ttctttcaat gacttctccg caatcgcggc aatacgggta
    20581 tcgggttgat tgactaatgc ctggtataaa taaaactggt aagtactgaa gaaaaactgt
    20641 cgcaatatcg tctgggccca atcgccattg ggttgttccg tcagcagtac atttttaaat
    20701 tcccgttcgg tccgcaggta agccagcgaa tcctccgttg cgccattacc cgtaagttcc
    20761 gccgcatact gataaaaact ccgcgcctgc ccgatcaggt ccagcgaaat attggtaata
    20821 gcgatatcct gttccaacac cggtccatga ccacaccagg cggagttgcg ttgaccgaga
    20881 atgagggcat tgtctgctaa gtaaagggtg taatttatta ttgaattcat tctttagtat
    20941 ttaagaacca agatgccgaa ttaagaacca agaagccaag atgccaagaa gccaaagaat
    21001 aatcaatgat caaaaaacaa ataaattcca attgataaat tccaaaaaag caccaattgg
    21061 ttattcttt
//
LOCUS       DAOPMW010000020        20926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2390_length_20926_cov_12.431316, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000020 DAOPMW010000000
VERSION     DAOPMW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_20926_cov_12.431316"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLU11_02150"
     CDS             <1..1152
                     /locus_tag="PLU11_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN57937.1"
                     /translation="FKQPPMSTSSHAEILEYLNHGDADLAVRRLLDYCLDTGQDALIS
                     EAIRLSQAYHQAIEIGPIPASFTEQARSLIDKAAATGQTNPVPQPLITADQVAKTYSG
                     GGFSLKPISFTLQTGQVLGVVGENGNGKTTLLRCLAGQLALDGGKVDYHLLRKPDYYA
                     IKNHIAFIPQRIPKWYGLLKDNLHFSAAISGIHGPENDRMVDFMLERLNLSRYAHLTW
                     SQISSGYRTRFEIARILLQRPRLLILDEPLANLDINAQQTILTDLVFMARAAHNPMGI
                     VLSSQQLHEVEKVADTVIFIKDGNCLYSSADKAEKITSTAIEFETMLDRESIHRLFAA
                     GSVSLQYNGGFYTIESTGLSAAEILATLVNAKVPLTYYRDITYSTKRFF"
     gene            1168..2682
                     /locus_tag="PLU11_02155"
     CDS             1168..2682
                     /locus_tag="PLU11_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57938.1"
                     /translation="MDNVNRPLIPLFLKKLDATLLRKAPAIWQSRFHLVVYYTILVAV
                     AVFGLSFISFGNPKDDTGTSGLTTFTVMISLLGLVIWIIYLLRFNVFKRFGSWRVTDS
                     LISFLFYFLSVASIVSICFIPAAVQSLRANQQYGNEELVADINEINTTACQLEYEILD
                     REWKPDTTRLISSWEENAIRVSVDTSAEIANVKTDHFNKIDTSDLRRRLANADSVVQL
                     TDSLFIFYSVPDYLFVNNYSSDQYSVTKVLSSAEIYRKYLQTRPNPDRPLLVARLKAL
                     AEKWAAPGQYEYTDSPVNENEEYYLSRIKRKYGLFSADAGVGNVTEKKYNWKENRLIY
                     LRILYYISVSLALLLFLFRHSSTRTFFFSLLTAVLLSILTGLLMIMGGAGTDGENFLV
                     ILTFYYLFFAAISISIFSTGSRTIFQGIGLNLFVYMTPFMPLVVATLYVISQTKRRLL
                     HYLGEPGNPAVTADYFDSGPVYRAAEWLGPIIFLLLLQFLFSRLYRRWYAAPEN"
     gene            complement(2691..3503)
                     /locus_tag="PLU11_02160"
     CDS             complement(2691..3503)
                     /locus_tag="PLU11_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPN57939.1"
                     /translation="MKRLLFLSFVLFFTSPLFAQETKPLTIGEIKTLKSAVLGEDRTL
                     NINLPLSYDTSKAYPVIYVLDGSMNEDFLHITGLLQFFNMTFSMPDCIIVGIANVDRK
                     RDFTFHTELKDLKKDYPTTGHSEKFIRFIESELQPYISKQYKTTDTRYLIGQSLGGLL
                     ASEILLKHSTLFTHYLIVSPSLWWDDESLLKAAPELVAKQDMSGTFVYVSVGKGEDKI
                     MQREASELYGLLKKANKPGLQTAFKLMTEDNHATILHRSLYEGLLILFPYKE"
     gene            3663..5891
                     /locus_tag="PLU11_02165"
     CDS             3663..5891
                     /locus_tag="PLU11_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPN57940.1"
                     /translation="MYRFAILLLAVVSGNLIFAQTSTDKKIEKSVDSLLRLMTLEEKI
                     GQMNQYNGPWAATGPLTNDDNLLDQVKTGKVGSMLNVTGVKRTRELQELALQSRLKIP
                     MLFGQDVIHGFRTIFPIPLGEAASWDLKAMEDGARVAATEASAAGVHWTFAPMVDIAR
                     DPRWGRVMEGAGEDTYLGSLIAKARVKGFQGTGFGDTSAVMACAKHFAAYGAAVGGRD
                     YNSVDMSLRQLHETYLPPFKAAVEAGAATFMNSFNDINGIPATGNKYIQRDILKRDWK
                     FTGFVVSDWGSIGEMINHGYSKDSKEAGMQAANAGSDMDMESRSYTKYLSALVKEGKV
                     KMAVIDDAVRRILRKKFEMGLFTDPYKFSNEKREQTQWNNLQHRVVARDMAKKSIVLL
                     KNEKQVLPISKQTGSIALIGPFVKAKSDHLGFWSYDWPDDSTRIVSLWEGVKAKVAPS
                     TKMLYAKGCGITDLDKTGFAEAVETARKADYVILTIGETRDMSGEAKSRSSIQLPGVQ
                     EDLVKEIVALGKPTVVMISAGRPLVFNWTADHAPAILYTWWLGTEAGNAMADVLFGDY
                     NPSGKLPMSFPRTEGQIPIYYNHYNTGRPAKNDSDRNYRSAYTDLSIYPKYAFGHGLS
                     YSNFEYSDITLSALSFTASQRLTATVTLTNKSSRPGTETVQLYIRDITGSVVRPVKEL
                     KGFQQVLLNGGETKTISFTLTANDLRFYNDQLQYIYEPGEFKLFIGSSSDQVKEVGFE
                     LK"
     gene            complement(6285..6530)
                     /locus_tag="PLU11_02170"
     CDS             complement(6285..6530)
                     /locus_tag="PLU11_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57941.1"
                     /translation="MKYVLMAIGLYILYQFIFKLVIPVFIATRKIKKGFREMQSRMQE
                     QMQQQQQQQSGYAQQPETSAPDSKFKSGDYIEFEEVK"
     gene            6713..7183
                     /locus_tag="PLU11_02175"
     CDS             6713..7183
                     /locus_tag="PLU11_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HPN57942.1"
                     /translation="MSNETQIKALEQKVLALISEEPDHFLVDIRIKPTNNFKIYIDGN
                     QGISVEKLVSYNRKLYKQLEEEGQYPEGDFSLELSSPGLDEPLKLHRQYLKNVGRFVE
                     VVMLDGTKAEGKLLSATDEEIVVEEIKGKGKKATTVLHTLPFSQIKTTKIQIKF"
     gene            7211..8458
                     /gene="nusA"
                     /locus_tag="PLU11_02180"
     CDS             7211..8458
                     /gene="nusA"
                     /locus_tag="PLU11_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPN57943.1"
                     /translation="MSSINLIDAFQDFKDAENIDRPTMMKVVEDVFKTLLRKKYGSDD
                     NFDVIVNAEKGDLEIIRRRMIVEDGQVEDPLAQVGYSDAVKIEPDFEVGEELYEEIDL
                     LDFGRRAILAAKQTLASRISDLKKNVLAKKYGDRIGEIISAEVYQVWKKEILLLDEEG
                     NELILPKSEQIPQDYFKKGENIRAVVARVDMKNNNPVIILSRTSPDFLAKLLEIEVPE
                     IFDGLIVIKKIVRDPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INYTSNIQLLIQRSLTPAKITSMELDNEAHHANVYLKPDQVSLAIGRRGVNIKLASEL
                     TGYELDVYRDNEGEEEEFDIDLEEFSDEIETWIIDELKRVGCDTGRSVLNLTPEELER
                     RTDLEKETIADVRRILKEEFEKE"
     gene            8587..11463
                     /gene="infB"
                     /locus_tag="PLU11_02185"
     CDS             8587..11463
                     /gene="infB"
                     /locus_tag="PLU11_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPN57944.1"
                     /translation="MAELKLPRLLAAAKEFNVGQDTIIDFLIGKGFPKDDLKPTSKLS
                     EDMYRALQQEFQGDKAAKMKSDQLELPKGSQPDAKKKKEEEEISFKKEEKAPAKAAKK
                     EEEAKPAVAAVAEEKPVKEEVKEIAPEPAPEVEKAAEEPAKPETTGIEGPKVVTKIDL
                     SAIDSSTRPKKLVKAKKEEAAPAAEEAKPVAKPKLKKKEEEVVAEVPVVESTPVEEAP
                     VAEEQQAAVIENIEVEKLTGPKILGKIELPVDNDTRPVKDEKKKRKRIPIEKKDNKRE
                     IFINKEEDKRPGGGAGGFQRGRGGAAGGPHRGGPRPVLGRREDKLIDEKEIQRKIQET
                     QAKLSGGGGKGKSLKAKHRREKRQEMADAMGDAVDNKLQVTEFISVSELANLMDVSFA
                     EVISKCMSLGIMVSINQRLDAEVIELVASEFGFDVEFIDMEKQMEMEEEEDQDTEEEL
                     QFRSPIVTIMGHVDHGKTSLLDYIRNANVVAGEAGGITQHIGAYQVELQNGKEITFLD
                     TPGHEAFTAMRARGAKVTDIAVIVVAADDAVMPQTREAISHAQAAGVPMIFAINKIDK
                     DGANSQKIYEQLSQMNILLEEWGGKFQSQEISAKKGLNIDKLLEKVLLEAEILDLKAN
                     PDREATGTIIEASLDKGRGYVATLLVENGTLRHGDLIVSGQYYGRVKAMFNERNKKEE
                     EAGPSAPAVVLGLNGAPQAGEKFKVYADEAEAKEIANRRAQILREQGIRTKKHITLDE
                     IGRRLALGSFKELKVIIKGDVDGSVEALSDSLQKLSTPEILVSVIHKGVGQINESDIV
                     LAEASDAIVIAFNVRPSLQASRLADKAGIEIKMYSIIYNAIEEVKSAMEGMLEPKTQE
                     KIVANVEIREVFKFDKATVAGCVVLDGKIKRDSKVRLIRDGIVIYPTGEGVNAELGSL
                     KRFKDDAKEVVSGMECGLTIKNFSDIKAGDVVEAYEIEEVKRTL"
     gene            11773..13146
                     /locus_tag="PLU11_02190"
     CDS             11773..13146
                     /locus_tag="PLU11_02190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN57945.1"
                     /translation="MLSLKKIVTAVFLLLTFVGVAQGQPKKVIADKISGIVGDKIILY
                     SDIRNSLADIIRQGGQVPENAECMLMEQAIISKMLMLQAQKDSLPVTDEEVEADLDNR
                     IRTYINQFGSQEALEEVAGKTIYQIKDDARESVKEQKLAQAMQRKIVDNVRITPIEVK
                     AYFEKIPKDSLPFYETELEIGQIVLYPKASRDLEQYIAGEMNNYKKQIETKITDFCRL
                     AKQVSEDPGSKDRCGQYQINRTEKTWDPVFLSTAFRMKEGEISSPVKSKFGFHIIQMV
                     QRNGDDAIVRHILRIPPVTEEEINQAKGKLDTIRSKIIAGTLGFNEAATKYSDDEAAK
                     YTGPFITNREGSPFVTIDMLDKEMVAMLAKMKVGEFSQPTPFESEQGKKGVRIVYLKS
                     KSEPHRMNLKDDYSKISTFALEEKKSQTLDKWIKNKLPTYYVMIDPGTAEECPQLKKF
                     DGETKAF"
     gene            13357..16386
                     /locus_tag="PLU11_02195"
     CDS             13357..16386
                     /locus_tag="PLU11_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57946.1"
                     /translation="MFKLPDYFVASGQFYYLMIDPEGYVSSANPLFSRHFLVEDVVAN
                     RIHVSSLLIPSDTVQFLSVLKNSLGQPHQLFPIQLIMRRVDGKPVALNWECSAHSNEL
                     GMPLYMQAISTASPIPGMNDRAVELKAGSHERYMAFEHSAEGLWMFDSKEPVSIHESA
                     DNILNYWKDHSTLVECNDNMARMYGFENAEELKGVKLGKLLDLSEPSSLEGLRTFIQN
                     GFATTTVETKEYDKSGNVKYFLNRMTGVVEQEQLQRVWGTQQDITAQRLVEEKLRQSE
                     FFFRNLFVNSLDGIFITDANGVITFVSPSVTPILGYEDEETLGHNCFDYVHPEDIKLA
                     RTAFETERKDETIVEFISIRVRQKGGDWLWCIVRGHNLLDNPYVKGMVINFYNDSFRK
                     KTENALKESEARFRYQATILNNVTDVIVTTDNARVITSWNHVIEKLTGITSAEAVGKA
                     YRIVVKTDYSPYTSEQVLEIVGREGIWKGEASFTGIDGEKKILLHTISMLYDEQGNKI
                     GLLGVGKDITDRKKAQARLQESELFYRNMITHSLDGIVLTDKTGSIRYCSPSIQRISG
                     YETAYLLGRKIFEFVHPSDTKVAFEAFDTEMRKESQLNYLTLRLKHANGDWVWCTARG
                     HNLINNPIFNAMVIYFTNDSKRKAIEDKLRESEQRFRSLVQNVQMGVTMREPSGKLIM
                     ANRAAEEMLGVGQDELSCTFENTDDWNTISEDGTPMPIDAHPVPVSIRTGSPVRGIVM
                     GVYRKKHNDRVWILVNTEPIVNDEGQLIYVICSFMDITEQKRMAQLLIDQEIQKQKQV
                     TQATIDAQEKERTEIGKELHDNINQHLNTTRLYLEVAKEKAGGEVLQMINLAHKNMAG
                     IINEIRQLSQSLVPPTLGDLGLAESIQDLCDGLRRSHKFKIKFSRSHFEEEIIPDNLK
                     LSLFRILQEMISNIIRHSGATQISIKLQTDAESIFLNITDNGNGFDTQSAREGLGLNN
                     IRTRVALFNGKMELKTAPGEGCVLHVTIPYHRESEAD"
     gene            16538..18265
                     /locus_tag="PLU11_02200"
     CDS             16538..18265
                     /locus_tag="PLU11_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPN57947.1"
                     /translation="MEKQHNRGQDGAGIATVKLDVEPGYPFMNRLRSNAPQAIADIFQ
                     QIGKEIDELERYHPDIKSHPGLMKGHVAFLGELLLGHLRYGTQGKNKLEYCHPFINRD
                     TIPARNLALAGNFNIVNSDELFTLTGKEPHPNVRFSDLAAMIELMHTYLCQEDETSPE
                     KLDIKRVLKKTLPLMDGGFHVGGVTGNGIGFVFRDAHGIRPSYYYINDEVVVAASERA
                     AIRTTFNVGENEVKELMPGQALIVSETGEIEIAQILEPKERKACSFERIYFSRGSDEK
                     IYKERKALGYNLSEPVLHAIEHDLKNTIFSFIPNTAETAFYGMLKGMENYLNKVKLER
                     ILSWGKDYDDEKLAEMINRRIRVEKIAIKDVKMRTFITEDVSRNEMVQHVYDITYGTV
                     RPGLDTLVVIDDSIVRGTTLKESIIRMLGRLQPKGIVVVSSSPQIRYPDCYGIDMSKM
                     GDFIAFNAAVELLKERNKYSLLNELLEKCKELESMNQLESENVVKQLYKQFTAEEIST
                     KIAKLITPPELSIPVKVIFQTIEDLHKACPNNLGDWYFTGNYPTPGGNKVVNKAFMNY
                     MEGKNVRGY"
     gene            18302..18799
                     /locus_tag="PLU11_02205"
     CDS             18302..18799
                     /locus_tag="PLU11_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPN57948.1"
                     /translation="MKTLILVRHAKSDWSGDGLPDFDRPLNERGKKDAPVMAARLLDK
                     KIPVDLFIASPAKRAAKTARFFAEAYGNTKADIVFKEELYLPAADVFYKVIEHISDLH
                     SHVALFSHNNGITDFANELTNTRIDNIPTCGIFALNIHCDHWKDFRVAQKEFWFFDSP
                     KSGQD"
     gene            complement(18800..19717)
                     /locus_tag="PLU11_02210"
     CDS             complement(18800..19717)
                     /locus_tag="PLU11_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN57949.1"
                     /translation="MFLFIFAPLKITSKAHIAVLFTNLFFALNLSLVKHISPSHVGAY
                     GLNIFRVGISLLLFWMVWAFGKTPAAIRRKDIPRFIACGLSGVAINQMLFVKGLTLTS
                     TIHASLLMLCTPLLITVFAFWVLKEKVTIQKALGLALGIGGALLLILSKEKNGTASLN
                     GDLFILVNAMAYTVYFIIVKPLMQVYPPLHVIRWVFTFGFFMILPFGWTEFAATSWPD
                     FEWTHFAALGMVVFCGTFLAYTFNIVGLKHLGAGITGSYIYTQPVFAAIIAALFLNEA
                     FTIEKIMAGLLIFSGVYLVSRKTPPTVEE"
     gene            complement(19717..20667)
                     /locus_tag="PLU11_02215"
     CDS             complement(19717..20667)
                     /locus_tag="PLU11_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN57950.1"
                     /translation="MFSEAFPELPLLHLPGYQVEYAKTKRGLFFKLLLQLPRIINSIW
                     QEGRWLKRKVAGHGIDAVISDNRYGLYHKKIPCIFITHQLCIKVPGSKRAERWLQKIN
                     YRFIQRFTACWVPDHAARPGLAGDLSHPDLQPAIPVTYIGPLSRFEKLNLPETAQHLL
                     LLLSGPEPQRTLLEEKLISELVHYNGTATLVRGLPGNTSMMPSTNMIRIYNHLPQQVL
                     NEELHKAGMVICRSGYSTLMDLAALNKKSILVATPGQTEQEYIAQLHLQQKTASAAEQ
                     SSFSLAEALDKASRFSFQPFPSSGKNELSAAVGRLLTSLR"
BASE COUNT         5922 a         4657 c         5331 g         5016 t
ORIGIN      
        1 tttaaacaac cacctatgtc cacctcttcc cacgcggaaa tcctggaata cctgaatcat
       61 ggagatgccg acctggccgt gcgcaggctg cttgattatt gtctcgatac cggtcaggat
      121 gcactgatca gtgaagcgat ccggctgagt caggcctatc accaggcgat tgaaatcggt
      181 ccgatccccg ccagttttac agaacaagcc cgcagcctga tcgataaagc ggcggctacc
      241 ggtcagacca atcccgttcc gcagcccttg atcaccgccg accaggtggc taaaacctat
      301 tcgggtggcg gctttagtct gaagccgatc agttttaccc tgcagaccgg acaggtgctt
      361 ggtgtggtag gggagaatgg taatggtaaa acgactttat tgcgctgtct cgccggacaa
      421 ctcgccctgg atggaggcaa agtggattac cacctgttgc gcaaaccaga ctattatgcc
      481 atcaagaacc atattgcttt tatcccgcaa cggattccca agtggtatgg tttattaaaa
      541 gataatctcc atttcagtgc ggctatttcg ggtattcatg gacctgaaaa tgaccggatg
      601 gtggatttta tgctggaacg gctgaacctg tcccgctatg cgcacctgac ctggagccag
      661 atcagcagcg gctaccgtac ccggtttgag atcgcgcgga tcttattgca aagaccccgc
      721 ttactgattc tcgatgaacc actggccaac ctcgatatca atgcgcagca aaccatcctg
      781 acggacctgg tctttatggc ccgggcggcg cataatccga tggggatcgt gctgagttcg
      841 cagcaattgc atgaagtgga gaaagtagcg gatacggtga tctttatcaa agacggcaac
      901 tgcctgtaca gcagtgccga caaagccgaa aagattacca gtacagcgat tgagtttgaa
      961 accatgctgg accgcgaaag tattcaccgg ctttttgcag cgggttcagt aagcctgcag
     1021 tacaacggcg gtttctacac cattgaatcc accggactca gtgccgctga gatactggcc
     1081 acgctggtca atgcaaaagt accgctgacc tattaccggg atattacgta cagtaccaaa
     1141 cgtttctttt aaacttattt tccttttatg gataacgtga acagaccttt gattccttta
     1201 tttttaaaga aactggatgc tacgctcctg cggaaggctc cggcaatctg gcagtcaagg
     1261 ttccacctgg ttgtctatta tacaatcctg gttgccgtag cggttttcgg actttccttt
     1321 atcagctttg gaaacccgaa ggatgatacg ggtacttcgg ggctgactac gtttaccgta
     1381 atgattagtt tgctgggtct cgtgatctgg atcatctacc tcctgcggtt taatgtgttc
     1441 aaacgtttcg gaagctggcg ggtgaccgac agcctgatta gttttctttt ttacttcctt
     1501 tctgtcgcaa gtattgtttc catttgtttt attcctgctg cggtgcagtc cctgcgggcg
     1561 aatcaacagt atggtaacga agaattggtc gccgatatta acgaaatcaa tacaacggcc
     1621 tgtcagctgg agtatgaaat tctggaccgg gaatggaaac cggataccac ccggttaatc
     1681 agtagttggg aagagaatgc catacgtgta tcagtggata cttctgccga aatcgcaaac
     1741 gtgaaaaccg atcactttaa taagattgat accagcgatc tccgccgccg gttggccaat
     1801 gcagatagtg tggttcaact gaccgactct ctttttattt tctattcggt gccggactat
     1861 ctttttgtaa acaattattc ttcggatcaa tacagtgtca ctaaagtgct ttcttccgca
     1921 gaaatttacc ggaagtacct gcaaacccgt cccaacccgg atcgtccgtt gttggtagcc
     1981 cggctcaaag cactcgccga aaaatgggca gcacccggtc agtatgaata cactgattcg
     2041 ccggtaaatg aaaatgagga atattatcta tccagaatca agagaaaata tgggttgttt
     2101 agtgcagatg caggagttgg aaacgtgacg gagaaaaaat ataactggaa ggaaaaccgc
     2161 ctcatctatc tgcggatatt gtattacatc tctgtttcac tggcattact cctctttttg
     2221 ttcaggcaca gcagcacccg tacgtttttc ttttcactat taacggcagt gctgctcagt
     2281 attctcaccg gcttgctgat gatcatgggc ggtgcaggaa cagatggaga aaactttttg
     2341 gttatcctga ctttttatta cctgttcttt gccgctattt ccatttccat tttttctaca
     2401 ggcagccgga caatattcca ggggatcgga ctgaacctat ttgtgtatat gactccgttt
     2461 atgccgctcg tagtggccac actctatgtt atttctcaga caaaacggcg cctgctccac
     2521 tatttgggtg aacccggcaa tccagctgtt acagccgatt attttgattc cggtccggtg
     2581 taccgggctg cagaatggtt gggacccatc atttttttgt tgctgcttca gtttttattc
     2641 agcaggcttt accggagatg gtatgctgct ccggaaaatt agtgtcccgg ctactcttta
     2701 tacggaaata aaatcagcag cccttcatac aaactccggt gcaggatcgt ggcatggtta
     2761 tcttcggtca tgagtttaaa agcagtctgc agtccgggtt tgttggcctt tttcagcaac
     2821 ccgtacagct cactggcttc ccgttgcatg atcttgtctt ctcctttacc aacagataca
     2881 tatacgaaag tgccggacat atcttgtttt gccactaatt caggggctgc cttcagcagg
     2941 ctttcatcat cccaccagag actcggactc acgatcaggt aatgcgtgaa taaggttgaa
     3001 tgcttcaaca ggatttcact ggccagtaaa ccacccagtg actgaccgat caggtagcgg
     3061 gtatccgttg ttttatactg tttgctgata tacggttgca gttctgattc gataaagcgg
     3121 atgaatttct cagaatgacc ggttgtcgga taatctttct tcaaatcttt cagttcggta
     3181 tgaaaggtga aatcccgttt ccggtccaca ttggcgatgc ctacgatgat gcagtccggc
     3241 atgctgaacg tcatattaaa aaactgcagc agcccggtaa tatgcaggaa gtcttcgttc
     3301 atgctgccat ccagcacata gatcacgggg tacgctttac tggtatcata ggataagggc
     3361 aggttgatat tcagagtccg gtcttcaccc agcacggcag atttcagggt cttgatttcg
     3421 ccgatggtaa gtggtttggt ttcctgggca aataaaggac tggtaaaaaa gagaacaaag
     3481 gataaaaaga ggagacgctt cataaaagcc ggattttttt tattttttaa agggtaaagt
     3541 taccggtctg tcaggaataa tccggctcct ggtttattcc tgatttttca tagtgaaaaa
     3601 cagctgtttt aatccggatt cccctcctga tttccgtacc tttaagcctc aattttccct
     3661 atatgtatcg ctttgccatc ctcctgcttg ccgtagtttc cggcaacctc atttttgccc
     3721 agacaagcac cgataaaaag atcgaaaaat cagtggattc cctgctccgt ctcatgacgc
     3781 tcgaagagaa aatcgggcag atgaaccaat acaatggtcc ctgggccgct accggcccac
     3841 tgaccaatga tgataacctg ctcgatcagg taaaaaccgg aaaagtaggt tccatgctga
     3901 atgtaaccgg ggtaaaaaga acccgggaat tacaggaact ggccctgcag tccaggttga
     3961 aaataccgat gcttttcgga caggatgtga ttcatggttt tcgcaccatt ttccccattc
     4021 cattgggaga agcggccagc tgggacctga aagcgatgga agacggagca agggtagcag
     4081 ctaccgaagc cagtgcggcc ggtgtacact ggacttttgc cccgatggtg gatattgccc
     4141 gcgacccccg ttggggacgg gtgatggaag gagccggtga ggacacttac ctcggttccc
     4201 tgatcgccaa agcccgcgta aaaggattcc agggaacagg cttcggggat accagtgctg
     4261 tgatggcctg tgcgaaacac tttgcggctt atggagcggc cgtgggcgga cgggattata
     4321 acagcgtgga catgagtctg cgtcagttgc acgaaaccta cctgccgcca tttaaagcag
     4381 cggtggaagc cggagcagct acgttcatga attctttcaa tgatatcaat ggcattccgg
     4441 ccacgggtaa taaatatatc cagcgcgata ttttaaaaag agactggaag tttactggtt
     4501 ttgtggtgag tgactggggc agtatcgggg agatgatcaa tcatgggtat tccaaagaca
     4561 gcaaggaagc cggtatgcag gccgccaatg ccggcagtga tatggatatg gaaagccgga
     4621 gttataccaa atacttatcg gccctggtca aggaaggaaa agtgaaaatg gcggtgattg
     4681 atgatgcggt acggcggatc ctgcgcaaga aatttgagat gggattattt accgatccgt
     4741 ataaattctc caatgaaaaa agggagcaaa cgcaatggaa taatctgcaa caccgggtgg
     4801 tggcccgtga tatggcgaaa aaaagtattg tactgctgaa gaatgaaaag caggtattac
     4861 ccatcagcaa acaaaccggc agcattgccc tgatcggtcc ctttgtaaaa gcaaaaagtg
     4921 atcacctcgg tttctggagc tatgactggc ccgatgattc cacccgcatt gtatccttgt
     4981 gggaaggggt gaaagcaaaa gtagctccct ccacaaaaat gctttatgcc aaaggatgtg
     5041 gcataacgga tctcgataaa accggatttg cggaagccgt ggaaaccgcc cggaaagccg
     5101 attatgtgat cctgaccatt ggtgaaaccc gcgatatgag tggcgaagcc aagagccgta
     5161 gcagtattca attacccggt gtgcaggaag atctcgttaa ggaaattgtc gccctgggca
     5221 aacccaccgt ggtgatgatc agtgccggtc gtccgctggt atttaactgg accgcggatc
     5281 atgccccagc cattctttat acctggtggc tgggtaccga ggccggtaat gccatggcgg
     5341 atgtactctt tggggattat aatccttccg gtaaactgcc catgagtttc ccgcgcaccg
     5401 agggacagat tcccatttat tataatcatt acaataccgg tcgtccggca aagaatgaca
     5461 gtgatcgtaa ttaccgttct gcctacacgg atctttccat ttacccgaaa tatgcattcg
     5521 gacacggact gagttattcc aactttgaat acagtgatat cacactcagt gccctatctt
     5581 ttacggccag tcaacgtctt accgccaccg ttacattgac caataaaagt agccgtcctg
     5641 gaacagaaac tgtacagctg tatatccggg acatcacggg ttctgtggta agacccgtaa
     5701 aggaattaaa aggtttccag caggtattgc tcaatggcgg agaaacaaaa acgatcagtt
     5761 ttacccttac cgccaatgat ctccggtttt acaatgatca gttgcagtac atctatgagc
     5821 cgggtgagtt taaattattc atcggcagca gttcggatca ggtgaaggag gtggggtttg
     5881 agttgaagta gctggttgct cgcccttcgt actcgtcggc ttcgatacgt cccgcctgga
     5941 tctgatcaac acccctgtaa atctgctttg gcgggactac tcagcctgac aacaacattt
     6001 cgtcagtttc gatctcctgt gtgacagggg gcaaaaattt tcgattctta ctgcttttat
     6061 tgttgagaag cggaatgccg ttgtcatggt gagcctgccg aaccatgata tgattttttt
     6121 aaatgattga taagcgatac caccctttcg aataggttct gcttttctgc ctacgaagcg
     6181 gaatgctgtt gtcatactga gtagactgcc tcttaacgat atgctaaggg gcagaaacaa
     6241 aagctactga ggcagtcgta tcgaagtatg agcgtgaaga acccctactt cacctcctca
     6301 aactcaatat aatcacctga tttaaatttt gaatcgggag cactggtctc cggttgttgc
     6361 gcatagccgg attgctgctg ctgttgttgc tgcatttgtt cctgcatacg gctttgcatt
     6421 tcgcggaatc cttttttgat tttccgggtg gcgataaaga ccggtatcac cagtttgaaa
     6481 ataaactggt aaaggatgta taaacctatc gccattaata cgtacttcat cggactgtaa
     6541 agataataag ggcctgtttc cggttaccgg cttttcgggt ccatttaccg atttattaac
     6601 gggttggttt tcaggaattg tatttgggat gaaatgtgaa aatcccttac atttgcactg
     6661 gaattagacg attgaaggga acggtgccgt tcccttcttc ttttatatac ccatgagcaa
     6721 cgaaacacaa ataaaggctt tagagcagaa ggtcctcgcc ctgatcagcg aagaacccga
     6781 ccatttcctg gtggatatcc ggatcaaacc gaccaacaat tttaagatct atattgatgg
     6841 taatcagggt attagcgtgg aaaagctggt tagttacaac cgcaaactgt acaagcaact
     6901 ggaagaagaa gggcagtatc cggaaggcga tttttcattg gaattatcct cccccggact
     6961 ggatgaaccc ctgaaactgc accggcaata cctgaaaaat gtgggtcgtt ttgtggaagt
     7021 ggttatgctg gacggaacta aagcggaagg gaaactgctc agtgccaccg atgaagaaat
     7081 tgtggtggaa gaaataaaag ggaaggggaa aaaagcaacg actgtgctgc atacacttcc
     7141 cttcagccag ataaaaacaa caaaaattca aattaaattt taatcaaacc ggattacaaa
     7201 attttgagct atgtcaagta ttaacctgat cgacgcattc caggatttta aagatgcgga
     7261 aaatattgac cgccccacaa tgatgaaagt ggtggaagat gtttttaaaa ccctcttgcg
     7321 taaaaaatat ggcagtgatg ataatttcga cgtgatcgtg aatgccgaga aaggagacct
     7381 tgaaattatc cgccgccgga tgattgtgga agacggacaa gtggaagatc ccctggccca
     7441 ggtgggttac tccgatgcgg ttaaaattga acccgacttt gaagtagggg aagaattata
     7501 tgaagagatt gacctgctgg atttcggccg ccgtgctatc ctggctgcca aacagaccct
     7561 ggccagccgg atcagcgacc tgaagaaaaa tgtactggct aaaaaatatg gcgaccgtat
     7621 cggtgaaatc atcagtgcgg aggtttacca ggtatggaaa aaggaaatcc tcctgctgga
     7681 tgaagaaggc aatgaactga tcctccccaa gagcgagcag attccccagg attatttcaa
     7741 aaaaggcgaa aacatccggg ccgttgtggc cagggtggat atgaaaaata ataatccggt
     7801 aatcatcctg tcccgtactt ctcctgattt cctggcgaaa ttgctggaaa tcgaagtacc
     7861 ggaaattttt gacggactga tcgtgatcaa aaagatcgtg cgtgatccgg gagaaagagc
     7921 gaaagtggcc gtggaatcct acgacgaccg tattgacccc gtaggcgcct gcgtgggtat
     7981 gaaaggaagc cggattcatg gtatcgttcg cgaactgaaa aacgagaata ttgacgttat
     8041 caattatacc agtaatatcc agttgctgat ccagcgttca ctgacaccgg ctaaaatcac
     8101 cagtatggaa ctggataatg aagcccatca tgcgaatgtt tacctcaagc ccgaccaggt
     8161 atccctggct atcggccgcc gcggggtgaa tatcaaactg gcatcagaat tgaccggcta
     8221 tgaactggat gtttaccggg ataatgaagg tgaggaagaa gaattcgata tcgacctgga
     8281 ggaattcagc gatgaaatcg aaacatggat cattgatgaa ctgaaacggg tgggttgtga
     8341 tacaggacgc agtgtactga acctgacacc ggaagaactg gaacgccgta cagacctcga
     8401 gaaagaaacg atcgccgatg tacgcaggat cctgaaagaa gaatttgaga aagaatgagc
     8461 attgtcataa cagtccggta gagttataaa ggtcatataa ctcaactggc agccttgacc
     8521 cgtacagctc gtttgaatga tatgacttgt ttgacctatt tgaccgtttt gatttaaaaa
     8581 acattaatgg cagaattaaa acttccacga ttattagctg cggctaaaga atttaacgta
     8641 ggacaggaca cgatcatcga tttcctgatc gggaaaggat tcccgaagga cgacctgaag
     8701 cccacttcaa agctgtcgga ggacatgtat cgtgcccttc agcaggagtt ccagggagac
     8761 aaggccgcaa agatgaagag cgatcagctg gaattgccta aaggcagtca gcccgatgcg
     8821 aaaaagaaaa aggaagagga agaaatcagc ttcaagaagg aagagaaagc tcccgcaaag
     8881 gccgcaaaga aagaagaaga agcgaaaccc gccgtggcgg ccgttgcaga agagaaaccg
     8941 gtgaaggaag aagtgaagga aattgcaccc gaacccgcac ctgaagtgga gaaagcagcg
     9001 gaagagcccg ccaaacctga aacaacagga attgaaggcc ccaaggtcgt tacaaagatc
     9061 gacctctctg cgattgactc ctccactcgt cccaagaagc tggtaaaagc taagaaagaa
     9121 gaagcagctc ctgcagcaga ggaagccaag cctgttgcca aacccaagct taaaaagaaa
     9181 gaggaagaag tggtggctga agtaccggtg gtggaatcca cgcctgtgga ggaagcaccg
     9241 gtggcagaag agcagcaggc cgctgtgatt gaaaatattg aagtagaaaa actgactggt
     9301 ccaaagatcc tcggtaagat tgaactgccg gtggacaatg atacccgtcc ggtgaaagac
     9361 gagaagaaaa aacggaagcg tatcccgatt gagaaaaaag acaacaaacg ggaaatcttc
     9421 attaataaag aagaagacaa acgtccgggc ggcggtgctg gcggattcca gcgtggtcgt
     9481 ggtggtgctg caggtggccc tcaccgtggc ggcccccgtc cagtacttgg acgtcgggaa
     9541 gacaaactca ttgatgagaa agaaattcag cggaagatcc aggaaacgca ggccaagctt
     9601 tccggtggtg gtggtaaggg caagagcctg aaagccaaac accgcaggga aaaacgccag
     9661 gaaatggcgg atgccatggg cgatgccgtt gataacaaac tgcaggtaac ggaatttatc
     9721 agcgtgagcg aactcgccaa cctgatggat gtaagtttcg cggaagtgat cagcaagtgt
     9781 atgagtctcg gtatcatggt gtcgatcaac cagcgtcttg atgcggaagt gatcgaactc
     9841 gtggccagtg aattcggttt cgatgtggaa ttcatcgata tggagaagca gatggaaatg
     9901 gaagaagaag aagaccagga tacagaggaa gaactgcagt tccgttcacc cattgttacc
     9961 atcatgggtc acgttgacca tggtaaaaca tctttacttg actatatccg gaatgcgaat
    10021 gtagtggccg gtgaggcggg tggtatcacc cagcacatcg gtgcttacca ggtagagttg
    10081 cagaatggaa aagaaattac cttcctcgat acaccaggtc acgaagcgtt taccgcgatg
    10141 cgtgcccgtg gtgcgaaagt gacggatatc gcggtgatcg tggtagcagc ggatgacgcg
    10201 gtgatgccac agacccgtga ggccatcagc cacgcacagg cggctggtgt accgatgatc
    10261 tttgcgatca acaagatcga taaggacggc gccaattcac aaaagatcta tgaacagctt
    10321 tcgcagatga acatcctgtt agaagaatgg ggtggtaaat tccagagcca ggaaatatcc
    10381 gccaagaagg gattgaatat tgacaaatta ctcgagaaag tattgctcga agcagagatc
    10441 ctggacctga aagccaaccc tgaccgggaa gctaccggta ccattattga agcctcactc
    10501 gataaaggac gtggatacgt agccaccctg ctggtggaaa acggaaccct gcgccatggt
    10561 gacctgatcg tgagtggtca gtactacgga cgggtgaaag ccatgttcaa cgaacggaat
    10621 aagaaagaag aggaggccgg accttctgca cctgcagtag tactcggatt aaacggtgcc
    10681 ccccaggccg gtgagaaatt caaggtttat gctgatgagg cagaagccaa ggaaatcgcc
    10741 aatcgccggg cacagatact gcgcgaacag ggtatccgca ccaagaaaca cattacactg
    10801 gatgagatcg gtcgccgtct ggcactggga agcttcaaag aactgaaagt gatcatcaaa
    10861 ggtgacgtgg atggatcggt agaagccctg agtgactctt tacagaaact gtctacaccg
    10921 gagattctcg tgagtgtgat ccataaagga gttggtcaga tcaatgaaag tgatatcgta
    10981 ctggctgaag catccgatgc gatcgtgatt gcctttaacg tacgaccttc cctgcaggca
    11041 tcgagactgg cggataaagc cggaatcgag atcaagatgt actcgatcat ctacaacgcg
    11101 atcgaagaag tcaagagtgc gatggaaggg atgcttgaac ccaagaccca ggagaaaatc
    11161 gtggccaacg tcgagatccg tgaagtattc aagtttgata aagcgaccgt tgccggttgt
    11221 gtggtactcg atggtaagat taaacgggat tcgaaagtcc gcctgatccg ggatggtatc
    11281 gtgatctatc cgacaggaga aggggtcaat gccgaactgg gttcactgaa gcgcttcaaa
    11341 gatgacgcga aggaagtggt atcgggtatg gagtgtggtc tgacgatcaa gaactttagt
    11401 gatattaagg cgggagacgt ggtggaagct tatgagattg aagaagtaaa gagaacgctt
    11461 taaaagcaac aagaaccgaa gatgccaaga agccaaagaa gcatcaagga acaagagaca
    11521 aggaagaacc aagaaccgaa gatgccaaga tccaaagaag caccaaggac caagagacaa
    11581 ggaagaacca agaaccgaag atgccaagat ccaaagaatg atcaagatac aagatgccaa
    11641 ataagcatca aatcagaaat tccaaacctt ctaagtgaga tcagcaaagg ggtttggaat
    11701 ttatgttaaa acctttgtta aataaagcac cggtgatacg attgacagtg ccggtaaact
    11761 acttttgaaa ttatgcttag tttgaagaaa attgtaacag ccgttttttt actcctcact
    11821 tttgtcggtg tggcccaggg tcagcccaaa aaagtgattg ccgataagat ctccgggatt
    11881 gtgggagata agatcattct gtattcggat atccgtaact cgctggctga tattatccgc
    11941 caggggggac aggtgccgga gaatgcggaa tgtatgctga tggaacaggc cattatttcc
    12001 aaaatgctga tgctgcaggc gcaaaaggat tcactgcctg tgaccgatga ggaagtggaa
    12061 gccgatctgg ataacaggat ccgtacctat atcaatcagt ttggcagcca ggaggcactt
    12121 gaagaagtag caggcaagac catctaccag atcaaggatg atgcccgtga gtcggtaaag
    12181 gaacagaagc tggcccaggc gatgcaacgg aagatcgtgg acaatgtgcg gatcaccccg
    12241 atagaagtaa aagcctattt tgaaaaaatt ccaaaagaca gtttgccgtt ttatgaaacc
    12301 gagctggaaa tcggacagat cgttttatat cccaaagcat cccgcgatct cgaacagtat
    12361 attgccgggg agatgaataa ctacaaaaaa cagatcgaga ccaagattac cgatttctgc
    12421 cgcctggcca aacaggtatc cgaagatccg ggaagtaaag accgctgtgg tcagtaccag
    12481 atcaaccgga cagagaaaac atgggatccg gtattccttt ccactgcatt ccggatgaag
    12541 gaaggcgaaa tttcctcacc ggttaaatcc aaattcggtt ttcatatcat ccagatggtt
    12601 cagcggaacg gggatgatgc tattgtacgt catattttac gcatcccgcc ggtaaccgaa
    12661 gaagaaatca accaggcaaa aggcaaactc gataccatcc gttcaaaaat catcgcgggt
    12721 actctgggtt tcaacgaagc cgctaccaag tacagtgatg atgaagccgc caaatacacc
    12781 ggacctttta tcaccaatcg cgaaggctct ccctttgtta ccattgatat gctcgataag
    12841 gaaatggtgg ccatgctggc gaaaatgaaa gtgggtgaat tttcacagcc gacgcccttc
    12901 gagagtgagc agggtaagaa aggcgtacgg atcgtttacc tgaaatcaaa gtcggaaccg
    12961 caccggatga atctgaagga tgattacagc aagatctcca cgtttgcgct ggaagaaaaa
    13021 aagagccaga cccttgacaa atggatcaag aataaactcc cgacctatta tgtcatgatt
    13081 gacccgggca cggctgaaga atgtccccag ttgaaaaaat ttgatgggga aaccaaagcg
    13141 ttctaaacgg gtataacata ttgcgaaacg gctgtcttaa cgagacagcc gtttttttat
    13201 gcgcctgaca gccgggattt acctgaataa caatagtaaa aactgcaggt tttggtgacg
    13261 agcttcatta gacgtttcta aaatcgtgtt gattatattt ccttcactca atttaaacat
    13321 taactttagt cttcagttca atggttgaat ctacatatgt ttaagttgcc tgattacttt
    13381 gtcgcatcgg gtcagtttta ctatctcatg attgatcccg aagggtatgt ctcatcggca
    13441 aacccattat tctcccgcca tttcctggtg gaagatgttg tggcaaaccg tattcatgtc
    13501 tcctccctgc ttattccttc cgataccgtg caattccttt ctgtattgaa aaacagcctg
    13561 gggcaacccc atcagctttt cccgatacaa ttgataatga gacgggttga cggtaagcct
    13621 gttgccctga actgggagtg ttctgctcat tccaatgagc ttggaatgcc cctttatatg
    13681 caggcgatca gtacggcatc tccaataccg ggtatgaatg acagggctgt ggaattaaaa
    13741 gcaggaagcc atgagcggta tatggcgttt gagcatagtg cagaaggcct ttggatgttt
    13801 gacagtaaag aaccggtttc cattcatgaa tcggcggaca atatcctgaa ctactggaag
    13861 gatcattcca cgctggtgga atgcaacgat aatatggcca ggatgtacgg atttgaaaat
    13921 gcggaggaac tgaaaggggt taaactgggc aagttgctgg atctgtcgga gccctccagc
    13981 cttgaaggac tgcggacatt tatacagaat ggatttgcca ccactacggt ggagacaaaa
    14041 gaatatgata agtccggcaa tgtcaaatat ttcctgaacc ggatgacggg tgtggtggaa
    14101 caggagcaat tgcaacgggt ctggggaaca caacaggaca taacggctca gcggctggtg
    14161 gaagaaaaac tcaggcagag tgaatttttt ttccgtaacc tttttgtaaa ctcattggat
    14221 ggaatcttca taaccgatgc gaacggggta atcacctttg tctcaccttc tgttaccccc
    14281 atacttggat atgaggatga agagacgctg ggacataact gctttgacta tgttcatccg
    14341 gaggatatca aactcgcccg tactgctttt gaaaccgagc ggaaggatga aaccatcgtt
    14401 gagtttattt ccatccgggt acggcaaaag gggggcgact ggctttggtg tatcgtgcgg
    14461 ggccataacc tgcttgataa tccctatgtg aagggcatgg tcattaattt ttacaatgac
    14521 agcttcagga aaaaaacaga gaatgcactg aaagaaagtg aagcacgctt ccggtaccag
    14581 gccacgatac tgaataatgt aacagacgtg atcgtaacca ccgataatgc cagagtgatt
    14641 acctcctgga atcatgtgat tgaaaagctg accggtatta cttcagccga agcagtggga
    14701 aaagcgtacc ggattgtggt caaaacagat tattcaccat ataccagcga gcaggtgctc
    14761 gaaattgtgg gacgggaagg aatctggaaa ggggaagctt cctttaccgg tattgacggg
    14821 gaaaagaaaa ttctcctgca taccatttcc atgttatacg atgaacaagg aaataaaatc
    14881 ggattgcttg gggtcggtaa agatattaca gaccggaaaa aggcgcaggc ccgcttgcag
    14941 gaaagcgaat tgttttaccg gaacatgatc acccattcgc tggatggtat tgtactgacg
    15001 gataaaaccg gtagtatccg ttactgcagc ccgtcgatcc aacggatttc cgggtacgaa
    15061 acggcctacc tgcttggccg gaaaatattt gagtttgtac acccctccga taccaaagtg
    15121 gcattcgaag catttgatac agaaatgcgt aaagaatcgc aactgaatta tttaacgctc
    15181 cggctgaaac atgccaatgg cgattgggtc tggtgtacgg cccggggtca taacctgatc
    15241 aataatccca ttttcaatgc catggtgatt tatttcacca atgactcaaa aagaaaagcc
    15301 attgaggata aactgcggga aagtgaacaa cgcttccgta gcctggtaca gaatgtacag
    15361 atgggtgtta ccatgcgtga accttccggg aaactgatca tggccaaccg ggctgctgag
    15421 gaaatgctgg gtgtcggaca ggatgaacta agttgtacat ttgaaaatac ggatgactgg
    15481 aacacgatca gcgaagatgg tacgccgatg ccgattgacg ctcaccctgt tccggtctcc
    15541 attcgtacag gaagtcctgt gcggggcata gtgatggggg tataccggaa aaaacacaat
    15601 gaccgggtct ggatactcgt gaataccgaa cccattgtaa atgatgaagg tcagctcatc
    15661 tatgtcatct gttcttttat ggatatcacc gaacagaaaa gaatggctca actcctgatc
    15721 gatcaggaga tccaaaagca aaaacaggtg acccaagcca ccattgatgc ccaggaaaaa
    15781 gagcggacgg aaatcgggaa agaactgcat gataatatca atcagcacct caacaccacc
    15841 cggctttacc tggaagtggc caaagagaaa gcaggcgggg aagtgttgca gatgatcaac
    15901 ctggcacata agaacatggc cggtattata aatgagatcc gtcagctatc ccaatccctg
    15961 gtaccaccta ccctcggaga tctgggtctg gccgagtcga tacaggacct ttgtgacggg
    16021 ctgcggcgtt cgcataaatt caagatcaag ttcagccgtt cgcattttga ggaagaaata
    16081 attcccgata acctgaaact cagccttttc cgcatccttc aggaaatgat cagtaatatt
    16141 atccgtcatt ccggggccac ccagatttcg atcaaattgc aaacagatgc cgagtccatt
    16201 ttcctgaaca ttacagataa cggcaacgga tttgatactc aatctgcccg cgaaggactc
    16261 ggattgaaca atatccggac ccgggtggcc ttatttaatg ggaaaatgga actaaagacc
    16321 gctcccgggg aaggatgtgt gctccatgtg acgattcctt atcaccggga atcggaagcc
    16381 gattaattaa cccgaaacgc ctacctttgc gccttccatg agcgacccga taaaacacga
    16441 atgcggactc gcattcatac ggttaagaaa gcctttttcg tactaccatc agaagtacca
    16501 taccgtactg tgggggctta ataagctgta cctgctgatg gagaagcagc ataaccgcgg
    16561 acaggacggg gccggtattg ccacggtaaa actcgatgtg gaaccgggtt atccctttat
    16621 gaaccggttg cgtagtaatg ctccccaggc catcgccgat atattccagc agattggtaa
    16681 agaaatcgat gaactcgaaa ggtatcatcc cgatataaag agtcatcccg gcctgatgaa
    16741 aggacatgtg gctttcctgg gtgaactcct cctggggcat ctccgttacg gtacccaggg
    16801 aaaaaacaaa ctcgaatact gtcatccgtt tatcaaccgg gatacgatcc ctgcccgtaa
    16861 cctcgcactg gccgggaatt tcaatatcgt taactccgat gaactgttta cccttaccgg
    16921 taaagaacct catccgaatg tacgtttcag cgacctggct gccatgatcg aactgatgca
    16981 tacttatctc tgccaggaag acgaaacatc gccggagaaa ctggacatta aaagagtatt
    17041 gaagaagacc ctgcccctga tggacggtgg ttttcatgta ggtggtgtga ccggcaacgg
    17101 tattggtttt gtcttccgcg atgcacacgg catccgccct tcttattatt atatcaatga
    17161 tgaagtagtg gtggcagcct ctgaaagagc ggccatccgc accacgttca atgtagggga
    17221 gaatgaagtg aaggaactga tgcccggaca ggccctgatt gtgagcgaga ccggagaaat
    17281 agaaatcgcc cagatcctgg agcccaaaga gcgaaaggcc tgcagcttcg aacgtattta
    17341 tttttcacgg ggcagcgatg agaaaattta taaggaacgg aaggccctgg gatataacct
    17401 gagtgagccg gtactgcatg ctatcgaaca cgacctgaag aatacgatct tctcctttat
    17461 acccaatacc gccgaaaccg ctttctatgg tatgctgaag gggatggaga attacctgaa
    17521 taaagtaaag ctggagcgga tactcagctg gggtaaggat tatgatgatg agaaactggc
    17581 ggagatgatc aaccgcagga tacgggtgga aaagatcgct atcaaggatg taaaaatgcg
    17641 caccttcatt accgaggatg taagccggaa tgaaatggtg cagcacgtat atgatataac
    17701 ttacggaaca gtacgtcccg gcctcgatac cctggtcgtg atcgacgatt ctattgttcg
    17761 gggcaccacg ttgaaagaaa gtatcatccg gatgctgggc cgtctgcaac ccaaagggat
    17821 cgtggtggtt tcctcctccc cgcagatccg ctaccccgat tgttatggga tcgatatgag
    17881 taagatgggt gattttatcg cttttaatgc ggcagtggaa ctgctgaaag aaaggaataa
    17941 atactccttg ctcaacgagc tgctggaaaa gtgcaaggaa ctggaaagta tgaatcaact
    18001 ggaaagtgag aacgtggtga aacagttata caaacaattt accgcagagg aaatttccac
    18061 caagatcgca aaactgatca ccccgcccga actgagtatt ccggtaaaag taatcttcca
    18121 gaccattgaa gacctgcaca aagcctgtcc caataatctc ggtgactggt actttaccgg
    18181 aaattatcca acccctggtg gtaataaagt ggtgaacaaa gcctttatga attatatgga
    18241 aggcaagaac gtaaggggat attaagtctg tatttgcatt tcctgtttaa aaaatttcaa
    18301 tatgaaaacc ctgatcctgg tccgtcatgc caaaagtgac tggagcgggg atggtcttcc
    18361 ggactttgac cgcccgttga atgaacgggg aaaaaaggat gccccggtca tggctgcccg
    18421 gttactggat aaaaaaattc cggtagacct gtttattgcc agtccggcta agcgggcagc
    18481 gaagaccgcc cgtttttttg cagaagctta tgggaatacc aaagcagaca ttgtttttaa
    18541 agaggaactc tacctgccgg ctgcggatgt tttttataaa gtcatcgaac atatcagcga
    18601 tctgcatagc catgtggcgt tgttttccca taacaacggg atcacggatt ttgccaatga
    18661 actgaccaat acccgtattg ataatattcc cacctgcggc atttttgcgc tgaatatcca
    18721 ttgcgatcac tggaaggatt tccgggtagc ccaaaaggaa ttctggtttt ttgattcgcc
    18781 gaaatccgga caggactgat tattcttcaa cagtaggtgg tgttttacgg ctgaccaggt
    18841 atacccctga gaagatcagt aaacccgcca ttattttctc tattgtaaaa gcttcgttca
    18901 gaaacagggc cgcgataatg gcggcaaaga ccggctgtgt atagatgtat gaaccggtta
    18961 taccggcacc caggtgtttc aggccaacaa tattaaacgt ataagcgaga aaggttccgc
    19021 aaaaaaccac catgcccagt gccgcaaaat gagtccattc aaaatccggc cagctcgtgg
    19081 ccgcaaattc agtccagcca aaaggcagga tcataaaaaa tccgaaggtg aaaacccagc
    19141 ggatcacatg caagggcgga taaacctgca tcaggggttt tacaatgatg aagtagacgg
    19201 tatacgccat ggcatttacc aatataaaca gatcgccgtt taaactggcg gtaccattct
    19261 tttctttcga gagaatcagc agcagggcac caccgattcc caatgcaagc ccgagcgctt
    19321 tctgaatggt gactttttct ttcaataccc agaaagcgaa aaccgtgatc agcaaagggg
    19381 tgcagagcat gagcagcgaa gcatggatgg tggacgtaag ggtcagtcct tttacaaaca
    19441 acatctggtt gatggccact ccgctgagtc cgcaggcaat aaaacgggga atatcttttc
    19501 gccggattgc tgccggtgtt tttccaaagg cccagaccat ccagaaaagt aaaagactga
    19561 tgcccacccg gaaaatattc aggccataag cacccacatg tgaaggagaa atatgtttca
    19621 ccaggctcag gttgagcgca aaaaaaaggt tggtgaataa aacggcgata tgggccttgc
    19681 tggtgatttt caacggggca aagataaaga gaaacatcag cggagtgaag tcagtaagcg
    19741 acctactgct gcactcaatt cattttttcc ggaagaagga aagggctgga aggagaaccg
    19801 gctggcttta tcaagtgcct cagccagtga aaaagaggac tgctcagctg cactggccgt
    19861 tttctgttgc agatgtagct gagcgatata ttcctgttcg gtttgtccgg gtgtggctac
    19921 cagtatactt tttttattca gcgccgccag atccatgagg gtgctgtagc cgctccggca
    19981 gatgaccata cctgctttat gcagttcttc gttgagcacc tgctggggca ggtgattata
    20041 aatccggatc atattggtgg aaggcatcat cgatgtgttt cccggtaaac cccgcaccag
    20101 ggtagcggtt ccattataat gaaccagttc actgatcagt ttttcttcca ggagggtacg
    20161 ttgtggttca ggtcctgaaa gcaacagcag taaatgttgc gctgtttcgg gcagattcag
    20221 tttttcaaat cgcgaaagcg ggccgatata ggtaacaggt attgcgggtt gcagatcagg
    20281 atgggataag tcaccggcca gtccgggccg ggccgcatga tcaggtaccc agcaggcagt
    20341 gaagcgttgt ataaaccggt aattgatttt ctgtaaccat cgttcggctc ttttgctccc
    20401 gggtactttg atgcagagct ggtgagtgat aaaaatgcat ggaatttttt tgtgatataa
    20461 tccataccgg ttatcgctga tgaccgcatc gatcccgtgt ccggcgactt ttctttttaa
    20521 ccaccggcct tcctgccaga tgctgtttat aatacggggc agctgtaaaa gcagtttaaa
    20581 gaaaagacct ctttttgttt tagcatattc cacctggtag cctggtaagt gtaacagggg
    20641 gagttcgggg aaagcctcgc tgaacaaggc ggcctgggct ccttccccgg caagccagac
    20701 atcggccccc tgggcctgaa gttcccggat caggggaaaa cagcgggtgg catgcccgag
    20761 cccccagtcc agcggggcaa cgaggatacg gggttttcct tgtttttcag ggtatttggc
    20821 ctgatcaggc ttattatttg gtgaaaaatg gatcagcggt atactatttt gttcgtaaaa
    20881 tagttttaat ttagaacatt aagtgtatga aacaaatcaa caaaat
//
LOCUS       DAOPMW010000021        20315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2503_length_20315_cov_7.797285, whole genome shotgun sequence.
ACCESSION   DAOPMW010000021 DAOPMW010000000
VERSION     DAOPMW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20315
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_20315_cov_7.797285"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..2652
                     /locus_tag="PLU11_02220"
     CDS             490..2652
                     /locus_tag="PLU11_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57951.1"
                     /translation="MEHFDRIDEIVARITKEIERHITDLHTKVRQYGPFNVIANTSFR
                     NQLALLSPDAKPVAGSLSVASEYLSLICLKFPFTLGIREFTRPHDVAEDLHVINELCL
                     QVIQKYTMIHHHRFHVYRPDGTMSDIQHIAQALSSEELLVRNETFESHHWDLLEELYD
                     RYDDYFKKELGFTIKEAVRICITIADLVNKRMQESIMQLKSNSQQMLKEILAYKKSKK
                     KPQQFYPQEMLDAYCTWDDRALEMHFYESMATYQMVMMGDRLSFTPELIAEEENLEVE
                     TVRSFFSRLSLGFGEINADFSEPEIMHPLKDRPLIRHENQYMAPSLSLLDYSLDRLFA
                     GTLIKDGKKQARYMETRHDYLMEKGMEHIKSVLKGGQYYTHLKYPGGEMDGLVILGTN
                     SFFIEAKGHRIHDRAKKGYIDRLENHIDDLVKDSHYQATRTQQYLAGNPAAVFEDRQG
                     RKVTIDGTTFQNSFFISLTIESISAISCNLKLDNTLGLFTKETFPWIVSLYDLRVICE
                     HMEGPAYFIQYLHRRKEFFKHKKFKVLDELDLLGYYLKRNLRFDDIVQGQYDQLSGIL
                     LESMMEEYNKYYLYQEGVTKKPVQKLKHYSIQPVKTLVRALEESNLEFGLDAAVQLLE
                     FGSKTKKQILDNITTIKNRFKRDGYNHDFRIAGDDNDSDKSWMLSYWTGPHEDGFIEY
                     FRKYIRETFQREPVNSYIAILDTGKNEYQFAEIMLFTS"
     gene            2693..3853
                     /locus_tag="PLU11_02225"
     CDS             2693..3853
                     /locus_tag="PLU11_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57952.1"
                     /translation="MTTSEKFVADLCAMSFLPFWSFPGPIGKKGKELCDLLVVCHNTI
                     IIFSVKDITPSQHDDEVVVYDRWVKKAIIESISQIYGAERFINTVDSIQLKNSDHIIT
                     LPVKEKRIIHRVAIAFGSKDYYPLPMGDFENGYVNVFDEKATTTILKELDTVTDFTNY
                     LLAKQRFSEKHTIMLPYETDFLAFYLQTGLSLDVDEDVVLVGENLWEDYSKSEKYAQW
                     KTVIEVSYVWDMMIQRFYMDYVKPGVSHEKRRDMERAIRLINLEPRINRLELGLILEN
                     ASKTKVKARMIKPFDGAEHTYVFMPLTADNWEHKEAELGLRCIVARAQFPEAKKIIGI
                     AIGRTSEDNFTFDLHYLNMPEIDEKFIANADSIKEELGFFKKITQTHSKDMR"
     gene            3883..4893
                     /locus_tag="PLU11_02230"
     CDS             3883..4893
                     /locus_tag="PLU11_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57953.1"
                     /translation="MTYTEEIENAIAIKAVLPEDVYNFSESDYKDFFTGYYSFCRENL
                     DIQSKQEPIVPNVFLFTNSFEVNAGAGIRNKNFIIEINVGLIRSCFENYHDNKPLADF
                     FTQLLPDTFSKFDNTANVLAFQICTQFTYYHELAHLFQLSGKKENIAIKERYNLAGKA
                     CYDRVKHILEINADTYATINVATHIEQYIERTFKDKVTIENTMDTFVFFGCCLLNYTL
                     NFSTKPEEIYFEAHDHPHSFLRQLNVVLNLTHHVGHSPYFQKAGIVLNEGILFRSVID
                     IYKQLEKNGVFKTNVAGILDSSQQLAKPLANYLFSLLQFDTKDFVNAMDVWNKGATKD
                     KK"
     gene            6009..7430
                     /locus_tag="PLU11_02235"
     CDS             6009..7430
                     /locus_tag="PLU11_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57954.1"
                     /translation="MTVDKHPYSILAIEDNPGDYVLVTEFLEERISSCTIYHAPDFKT
                     ATGLLTSKAQSLDVILLDLTLPDKSGTELVMEILAMAGNLPVIVLTGNADIDFSIRSI
                     GFGVSDYLVKDDLTSDVLYKSIIYSIERKKIYKQLKESEERFSTLFHQSPQPMYVYDP
                     ATTKMVQVNKAATLFFGYSEEEMKGMSVMDLVFTDDVTMARHMAEEVLEDTSNSHAEL
                     LRFQKKTGEIVYMELYGTSINLEGKYLRSVIAIDITEKLLFEKKMTRAIIKTQEDERY
                     EIGSELHDNVCQILASSQMYMSMLEPELQQETVSLLEQAKGNTGLAIREIRNLSHRLA
                     PAFFDESSLEESMERLINDFSIGDEMQIDCVVSEQVLALDLGHDLQLNLYRILQEQLR
                     NIQKYAQASKVDICVDLENGKLNLQITDNGIGFDLSRVKKGIGIANMKRRAELFGGKT
                     VIFSEPGKGCQVLVTIPLNKQSS"
     gene            7630..12900
                     /locus_tag="PLU11_02240"
     CDS             7630..12900
                     /locus_tag="PLU11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN57955.1"
                     /translation="MATRNTTRNAGLTRKKKPAGLPSVLLSIGSEHKSFPFLKQLLTP
                     LLENSDAALLITNPLPEAVTLRLTEWLKLFKFNVRTAKDKTKTSARTLYLTPRGVRSF
                     ILDDVIRLEELNTPKEAKQKQDRLDAVLYDLNERIKEQACLYGITKLAATAPGLDELL
                     HGAVKLIPKGWQYPSHTCCSIIYNKAEFRSAGFRKTRWNQLIERDVQAGGKLVITVYY
                     KKKMPDADEGPFLKYERSLLNSIADSLILYISQLLVKSRSRQSESMLKEAQELALLGN
                     WSYDLLQKKSYWSDSVYDIFGVSRQQIRQSFGAFFNQVSKEDRKRVETTMRKARREGG
                     DFSLDYCILTPAGEKKYIEEFGYSEKDKKGRVIRLFGTVQDISQRMKAEQKLSDSEYK
                     YRSLFEASPVPSYIFDLHTHRILDVNKRMTEQYGYTRDELLSMTILDIRPKEQIPRLH
                     EQLEKANRVKGVIHFGVMTHQNKDGSLKQVDISGEQIRIGDSDCMLVLAHDISEKMMQ
                     QEMDHLERNIIEKSLKSKTAIDIILKDYLTGLEELITGLSASILRVSEGKVYNWASPS
                     LPLFYAEALNGLAIGPEEGSCGTAAYTGKQIIVSDIRKSNLWKKYKKLALSAGLRASW
                     STPVFNPEGKVIATFANYYKEIRMPSPFELEIFNRAAVLISILIESYEQDQRLQRSHE
                     RFGFVTKATSEAIWDWNLVTDKVFCGDGFNTILGVILKEQEWRLSVWLEYIHPDDRML
                     ITQKVESVLQGKENRWELEYRIIRKKGQLVYVSDRAYIIRNNQGRPIRIIGAIRDITV
                     KREEEQQLRLLESVVTNTSDAVVITRADLNTDQGPEIIYVNEAFSRMTGYSPAEVIGK
                     TPALLQGPKSDWAEMNRLSLALKRGEPCSVNIINYTKSGKEYRVEMMVKPVFDANHRI
                     THFFAIQRDITTQFKLQQQRRFMEMVSSTFKKIEGLVISLDSVLKKLVEFGDFTAAET
                     WLLTADKQHLSLVASFASTKASKDYYAENKALNTLTPDGGLLKEVWARRELVIVDDFD
                     KHASLRRRAGVQKAGFKSALAIPLMNRGELIGVLAVLTDKPSAHLSEFEQLFRGLEAD
                     LGSEIKRKQLENELSLIFNTAPDIIAVVGFDGYFKRINPAAYDILGYSIEELLSRPYN
                     EFIHQDYISATNSITATFPSISGSNYFENCYITKSGKSVWLGWTYQQIPGEDLVYTVA
                     KDITRQKELQRLLHDATSLARIGAWEMELPAMQIIWSDVTREIHEVEPGYQPVLQEIY
                     QFYQDSDKSRLEESVLKAIGEGIPYDLELLHVTAKGNQRWVRVIGTPEMANGKCLRIY
                     GSFQDIHARKLAEIELQQRTRYLSAIATVAQIFLSNENWFDGIKNSFDLARYTIMADQ
                     IFYKEIYTDKETGQSMYRQRIGWLKGKDMPAIDPVETEVLPIDSFPEVKEVLLSDQPY
                     MAVLSELPDNLLRQVLVHSGIKSVLFLPVFAGSVLEGVVGVEECYHERRWAEGEIFFM
                     RSLCSSLSSAIQRFNSSAAQKKLNEDLVRQTRELKISNTELEQFAYVASHDLQEPLRM
                     ITSFLTKLEDRYAIRLDDRGRRYIDFAVDGANRMRQNILGLLQYSRVGKGNAAIEEID
                     LNMVVEEIRLLHQTDINETGAVVEVAELPVINNYMAPVVQLMNNLVSNALKYRKPDTP
                     PVIRISARPVEEYWEIAISDNGIGIEKEYLQKIFVIFQRLHPKDQYEGTGIGLAVVKK
                     IVENLGGKVWVESDPGIGSTFYFTIRSIKD"
     gene            12961..13686
                     /locus_tag="PLU11_02245"
     CDS             12961..13686
                     /locus_tag="PLU11_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPN57956.1"
                     /translation="MDKDEEIALLKAQLQQSRDSFELFAYAVSHDMREPVRMVKSFMD
                     LLLKKYSDSLNEKAKSYVSYASDGAERADLMIQDLLFYYRAVKNIQPTTADLNIAYAG
                     ASGKLKRKLETRNCMISCPVLPVVQGDVDGIQEVFTYVIDHLLFHVPLDEQPRLQIGV
                     KQADKKWEITCTGNVPDPVPGELRDLFRLFHNRSQQLSADSTLNKLAVAKRIIEYLNG
                     EMKADQAAGGGVEFKITLPMVTE"
     gene            13702..15018
                     /locus_tag="PLU11_02250"
     CDS             13702..15018
                     /locus_tag="PLU11_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN57957.1"
                     /translation="MKLISAYKQKIRQNCIDSGAGKTDRVEYWQDTLFSATMEIMIPL
                     SLIAVIPGVIFSFTSKVPLLAILDLVAFLWLWLVGFARLPSVNTRKFLFVFCLYYVAS
                     YMLLYLGINGPGLLFLFAAAIFALLILPVSYAWWWSCINLSACLIFALVLHWDMAPFA
                     VVNEIPVSEWMVVSSNLVFLSFTFSFLLPKLMNGLSGSMEKQRLMQMDLFDKNSNLEQ
                     YASIISHDMQTPLRTISGFLNLLEKKYGNSLDDKGKEYIHFAVDGSAHLQHIITGLLD
                     FSRAGKYDAGKFEEFSLREVAGQVIQLLKGQLEEKAGEVRCESELMLFSNRSAFMQVL
                     HNLVGNAIKYSRKDVPPRISISVQDGIADWTIKVEDNGIGIEEEYFEKIFIIFQRLHS
                     RMEYEGTGIGLAIVKRIIEKLNGRIRVESTPGAGSSFYFTIPKQND"
     gene            15021..15461
                     /locus_tag="PLU11_02255"
     CDS             15021..15461
                     /locus_tag="PLU11_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN57958.1"
                     /translation="MKEVHILLVEDNEGDIVLTLEAFEESKILNKFSVVRNGKDALDF
                     LFKQAPYEDAKEPDIILLDINLPLKSGIEVLQEVKNDDRVRHIPVIMLTTSSSEKDIS
                     LSYKHHANCYITKPVEVFKFLEAVNAIEHFWLQIVSLDGKNKRE"
     gene            complement(15479..16168)
                     /locus_tag="PLU11_02260"
     CDS             complement(15479..16168)
                     /locus_tag="PLU11_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPN57959.1"
                     /translation="MAIDLYKKEDHLLLAVDCIIFGFDGSRLKALLVKRNLEPGLGKW
                     SLMGGFVKKTESVKEAATRVLNLLTGLTDVYMEELGCFGNVNRDPGGRVVSIAFFALI
                     KINDYSESLMKEHNSKWFELDHLPALIFDHGEMISQAKEKLKDKVSTHPAGFTLLPRK
                     FTLQQLQGLYEAIYEKEMDKRNFTRRILELGILKKLEEKEKESSRKGAFLFVFEKTRY
                     EKLSKKGFRII"
     gene            complement(16187..17887)
                     /locus_tag="PLU11_02265"
     CDS             complement(16187..17887)
                     /locus_tag="PLU11_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219851.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPN57960.1"
                     /translation="MSGISTLDILIFVIYFILVAGYGYWVYNKKKKATVTASHDYFLA
                     EGSLTWWAIGASLIASNISAEQFIGMSGNGYFVGIAVAAYEWIAALALIIIAVWFMPV
                     YLKNKIYTMPQFLKRRYNESVSLIMAIFWLLLYVFVNLTSILYLGAIAISGLIGPEYL
                     HVVMVGLAVFSLIITLGGMKVIGYTDVIQVAVLIIGGFATIYFALTIVSEKFGLGSDA
                     VAGFKTLMQQAPDHFHMILQKPTAASSQEDVNKYLILPGIAMYFAGQWIVNLNYWGCN
                     QYITQRALGADLETARKGILFAGFLKLLMPIIVMLPGIAAYVLHQNGHLESLRGMDDA
                     YSAILGFLPSGLKGLAIAALTAAIVASLAGKLNSIGTIFTLDIYMKYFKKEKVESNES
                     KEKNMVWVGRMAALVSIAIAVIFEWKDMLGISGEGGFTFIQKYTGFISPGVFAMFILG
                     MFWKRTTGTAAVAGLLTGFLLAIFFNSFAVPLMGNETILYTAFKNKDGIYEIPFLINM
                     GWAFFFTMVVMIGISLGGPKVNPKAFELDSEMFKVKPGILIMIVTTIMILAALYIKFW
                     "
     assembly_gap    18010..18109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18110..19176)
                     /locus_tag="PLU11_02270"
     CDS             complement(<18110..19176)
                     /locus_tag="PLU11_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPN57961.1"
                     /translation="MQLPDLKKWSVWFVTGSQHLYGEETLKQVDEHSAKIADTLNAAK
                     HIPVQVIFKPVVKTPEEIFNLINAANNDNSCIGIITWMHTFSPAKMWINGLKILRKPM
                     LHLHTQFNRDIPWSSIDMDFMNLNQSAHGDREFGFIMSRMRMNRKVVAGHWQDPEVQK
                     KINSWARAAAGWQDWQGARFCRFGDNMRQVAVTEGDKVEAEIKFGYSVNTYGVGDLVA
                     VINKVKNAAIDELIAVYNKEYKLAPSLKKGGKQYESLRDAAKIEIGMRTFLEQGGFKG
                     YTDTFEDLHGMKQLPGIASQRLMAEGYGFGGEGDWKTAALVRAMKVMGSGLPGGNSFM
                     EDYTYHFEPKNNLVLGSHMLE"
     gene            complement(19194..>20315)
                     /locus_tag="PLU11_02275"
     CDS             complement(19194..>20315)
                     /locus_tag="PLU11_02275"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPN57962.1"
                     /translation="ALALQLAACNNSTEKEQKDGTAQLNAKPGIGEMPFGTFEGKPVT
                     EYTLTNASGMQVGIINYGGAISKLITPDNKGKMGDVVFGFANLEGFQQKGNPYFGGLI
                     GRFGNRIAKAGFTLDGKKYTLAANNGVNALHGGSKGYDKVYWTIEKQAGDSSLKLTYT
                     SPDGEEGYPGTLKMEVIYTLTSANELKIDYSATTDKATPVNLTSHAYFNLSAGADSTI
                     LGHELMMNAAQYTPVDSTLIPTGKIEAVAGGPMDFNTAKPIGRDIDKITGGYDHNWVL
                     TRSGTGLELAAKLVHPGSGRVMEVFTTEPGLQFYSGNFLDGTLSNTKGGVKYVWHGAL
                     CLETQHFPDSPNQPSFPNTILKPGETYHHTCVYKFSTQP"
BASE COUNT         5846 a         4209 c         4677 g         5483 t
ORIGIN      
        1 gtttattcgc tagtttcatt agctggccaa tgcggagcca cctcctatac acttaagaga
       61 attttaggaa ggtatttggg caaagcatag ccagtttttc atttcaggca agaacggacc
      121 tggcaaagga gttgctaact actactaata atacacttca gatgattgcc gagagtgtag
      181 ggtacacttg gggaaccaat tttcagaacg tatttaaaag agttgttgga gcgacgcagg
      241 aagaatgccg aaaaaaaacc agtaatcaga gggttgacaa aagagaaaaa aaatagtggc
      301 catttccgta tctttgcagc gatgcaattc gttccaatca taggaaggtt tttccagacg
      361 cggtcgcagc tctgccgatc gatacttagt tcgtgggact ccctgtcctg atcagccctg
      421 tcattttact ttgctgattt tatttcaaca gcctgattta cttccttact ttatatagat
      481 aagaagcata tggaacattt tgatagaata gatgaaattg ttgcacgaat aacaaaagaa
      541 attgagcggc atattacaga cctacatacc aaagtacgtc agtacggacc ttttaatgtt
      601 attgctaata cctctttccg caaccaattg gcccttctta gtccggatgc caaacctgtt
      661 gcaggatcgc tttccgtggc ctccgaatac ctttccctga tctgcctgaa atttcctttc
      721 acattaggta ttagggagtt cacccgtccg cacgatgttg ccgaagacct gcatgtgatc
      781 aatgagcttt gcctgcaggt cattcagaag tataccatga ttcatcatca ccggtttcat
      841 gtatacagac cggatggtac aatgtctgat attcagcata tcgcgcaggc attatcaagc
      901 gaagagctgc tagtacgcaa cgaaaccttt gaatcacatc actgggacct tctggaagag
      961 ttgtatgatc ggtacgacga ttatttcaaa aaggaacttg gctttacaat aaaagaagcc
     1021 gtccgtatct gcatcacgat tgccgatctt gtgaataagc gcatgcagga gtcgatcatg
     1081 caactaaaat caaattccca gcaaatgctg aaggagatcc tggcttataa aaaaagcaag
     1141 aaaaagccac agcaattcta tccgcaggaa atgctggatg cttactgcac ttgggatgac
     1201 cgggcactcg aaatgcattt ttatgaaagc atggcgacct accagatggt gatgatgggt
     1261 gaccgtttaa gttttacacc cgaacttatc gcggaggaag agaatctgga agtagaaaca
     1321 gtacgcagtt ttttttcgcg gctctctctt gggttcggtg agatcaacgc cgatttttcc
     1381 gagcctgaga tcatgcatcc tttgaaggat cgcccgctga tcaggcatga aaatcaatat
     1441 atggctcctt cattgtcact gctggattat tcgctcgacc gtcttttcgc tggtacactg
     1501 ataaaagatg gcaaaaagca ggcacgttat atggagacca ggcacgatta cctgatggaa
     1561 aaaggaatgg agcatatcaa aagcgtcctt aaaggagggc agtattatac tcaccttaaa
     1621 tatccaggtg gtgaaatgga cggattggtc attctcggta ccaattcatt tttcatcgaa
     1681 gccaaaggtc accggataca tgaccgtgca aagaaaggat atattgacag gttggagaac
     1741 catattgatg atcttgttaa agactcccat taccaggcaa cccgtactca gcagtattta
     1801 gctggtaatc ctgcggccgt ttttgaagat aggcaaggaa ggaaagtgac gattgacgga
     1861 acaacgtttc aaaattcatt ttttatttcg ctaaccattg agagcatcag cgccatttcg
     1921 tgcaatttga agctggataa taccctcggt ctttttacga aggaaacatt tccctggatc
     1981 gtaagcctgt atgatctccg ggtgatctgt gagcatatgg aagggccagc ctattttata
     2041 caatacctcc accggcgtaa agagttcttc aagcataaaa aattcaaggt actggatgag
     2101 cttgatctgc ttggttatta cctcaagcgc aaccttcgtt tcgatgatat tgtccaaggc
     2161 cagtatgacc agctttccgg tatccttctg gaatcaatga tggaggaata caataaatac
     2221 tacctgtacc aggaaggagt cactaaaaag ccggtgcaaa aactgaagca ctattcgatt
     2281 cagcctgtaa aaacactggt ccgtgcactg gaagaatcga acttagaatt tggtctcgat
     2341 gcggctgtac agttactgga attcggatcg aagacgaaga agcaaatatt ggataatata
     2401 accacgatca aaaatcgctt caaacgggac ggctataacc acgatttccg aatcgcaggt
     2461 gatgacaatg attccgacaa aagctggatg ctgtcctatt ggacaggtcc gcatgaagat
     2521 ggatttatcg agtatttccg aaagtatatc agggaaacgt tccagcgtga gcctgtaaac
     2581 tcttatatcg cgatccttga tacaggaaaa aatgagtacc agtttgcaga aatcatgttg
     2641 tttacatcgt aagtagtttt tggggaagaa ctttaatgtg aaatttatta aaatgacaac
     2701 atcagaaaaa tttgtagctg atctatgtgc aatgtctttt ttaccatttt ggagttttcc
     2761 aggcccaatc ggaaagaaag ggaaagaact gtgcgatctt ttggttgtct gtcataatac
     2821 gatcataatt ttttcagtga aagatattac accctcccag catgatgacg aagtagttgt
     2881 ttatgatagg tgggtaaaga aggcaattat agaatccatt tctcagatat acggcgcaga
     2941 aagatttatt aatactgttg acagtattca gcttaagaat tcggatcaca ttatcacact
     3001 gccagtaaaa gagaaaagga tcatacacag ggttgctatc gcgtttggca gcaaggatta
     3061 ttatccgcta ccaatgggag attttgaaaa tgggtacgtc aatgtgttcg atgaaaaagc
     3121 tactactacc attttaaaag aactggatac tgtcactgac ttcaccaatt atttattggc
     3181 gaagcaaagg ttttctgaaa agcacaccat catgctgcct tatgaaacag attttcttgc
     3241 tttttacctg cagaccggac tttcactgga tgttgacgaa gatgttgttc tggttgggga
     3301 aaatctttgg gaggattata gcaagagtga aaagtatgcc caatggaaaa ctgttatcga
     3361 agtgagttat gtgtgggaca tgatgatcca acgcttttat atggactatg tgaagcccgg
     3421 agtatcccat gagaaaaggc gggatatgga aagagcgatt cgtttgatca acctggaacc
     3481 ccggatcaac agattggaac ttggcctgat cctggagaat gcctctaaaa cgaaggtaaa
     3541 agcaaggatg atcaagcctt ttgatggggc agagcacacc tacgttttta tgcctttaac
     3601 cgccgataac tgggaacata aggaggctga gttggggctt cgttgcatag ttgcaagagc
     3661 tcagtttccg gaagcaaaaa agatcattgg tatcgctata ggtaggacct ctgaagacaa
     3721 ttttacattt gacctgcatt atctgaatat gccggagatc gatgaaaaat tcattgccaa
     3781 tgctgattcc attaaggaag agctgggttt ttttaaaaaa ataacgcaga cgcatagtaa
     3841 ggatatgagg taatctggct caatcaaaaa accaaattca atatgacgta tacagaagaa
     3901 atagagaatg caattgctat aaaagcagtt ttaccggaag atgtttataa tttcagtgaa
     3961 agcgattata aagatttctt cacgggttat tattcgtttt gccgcgaaaa cctcgatata
     4021 cagtccaaac aagaacctat agtaccgaat gttttcttgt ttaccaacag ttttgaagta
     4081 aatgcaggtg cgggaatcag gaacaagaat tttatcatag agattaatgt cggcctgatt
     4141 cgttcctgtt ttgaaaacta ccatgacaac aagccgttag cggatttctt cacccagcta
     4201 cttcctgaca cgttcagtaa atttgacaac actgctaatg tcctagcctt ccagatctgt
     4261 acgcagttta cctattacca tgaattggct cacctttttc aattaagcgg caaaaaagag
     4321 aatattgcta taaaggaacg atacaatctt gctggcaaag cctgttatga tcgagtgaag
     4381 catatacttg aaataaacgc agatacctat gcaacaatca atgtcgctac tcatatagag
     4441 cagtatattg aaagaacatt taaggataag gtgacaatag agaacactat ggatactttc
     4501 gtgttttttg gatgttgtct gctaaactat acgcttaact tttcaactaa accagaagaa
     4561 atatatttcg aagcgcacga tcatccgcat tcttttctcc ggcaactaaa tgttgtattg
     4621 aatcttacgc atcatgttgg tcacagccct tatttccaga aagcgggaat tgtattgaat
     4681 gaaggcattt tattccggtc agtgatcgat atttacaagc aactggaaaa aaatggtgta
     4741 tttaagacca acgttgcagg tattctggac tccagccagc agctggccaa gccactggcc
     4801 aactatctgt tcagtctttt acaatttgat acaaaagact ttgtaaatgc aatggatgtt
     4861 tggaacaagg gcgcgacaaa agacaaaaaa taagcatgcc tcaaccttta gttgtgacag
     4921 gagaattcga atgagaatta tcgatgtata agcagcagct tgcggcaaaa gccgaaagcg
     4981 atagtattca ttcctgagtt tttatccgaa tatgcaaatg gaggacttcc tgcgccttcg
     5041 gtcaaatggc acctgtctat tgatatggaa atgggtggtc gactttattc gactagtatt
     5101 aatcgattga caaataatca ccattgctgt aggggataat cacttttgta cctattgaat
     5161 taaccctttc aagccaaatt ggcagaaaat taatgtaaga atatctatgg tataatttct
     5221 gggaatctgt ccattacaac taattcaaaa aattaagaat catggataat acagctgttt
     5281 tgagaaaagc attggcgaat ttaaactcaa aatttgttcg tgaaggacaa atttgtattc
     5341 aatcaactac attttccagt tatctaatta ctgatttcga gaatgatctc tttttttccc
     5401 tgtcaggaaa ctattatgga atagattgtt ttaagacttg ggaagggatt tactatgatc
     5461 agaaaatgga tttataattc tatgtgggga tgtcaggtcc tgataatttc tcaaatataa
     5521 tgtctggttt cccaatacaa ggcgatatgc ttaacctatt aacaacggag ctaaaaattc
     5581 gaataatgga agctagtttg agttatagta gaatttgtgt caactacgct gaattgccca
     5641 attttttgaa agttcctagc tagcatttct tatcatatgt cttttattga atttgcggcg
     5701 ataatgataa ttttcgaaaa ggagagggat gatatagagc atcaaggaat gattattaat
     5761 cgtcacatta aatttttatc ttgataaaat tttgttcttc ttttcctttg tttgaccttt
     5821 tccagttgcc gcattggtgc cttattgcgg aatgcattga acatcagctg aaaggaattc
     5881 ctgtatcggg aagtaagtaa gtattcagca agccctaacg gtaaacccct attgttcaat
     5941 cagttttata gaattaattt caagtgggat tccctgagca ctacaggata gaaattctcc
     6001 accaattcat gactgttgat aaacaccctt atagtattct ggccattgaa gataatcccg
     6061 gggattatgt gcttgttacc gaatttctgg aagaacggat ttcttcctgt accatttatc
     6121 acgcgcctga ttttaaaaca gccaccggat tgctgacgtc gaaggcacag tcgctggatg
     6181 tgatcctcct tgacctgacc ctgccggata aaagcggcac cgaattagta atggaaattc
     6241 tggccatggc cgggaacctg ccggtgatcg tactgaccgg aaacgccgat atcgatttca
     6301 gtatccgttc aatcggtttc ggggtgtccg attacctggt aaaggatgat cttacttcag
     6361 atgtcctgta caaaagcatt atctactcca tcgaacgaaa gaaaatatac aaacaactga
     6421 aagagtcgga agaacggttc agcaccttgt ttcaccaaag tccgcaaccg atgtatgtgt
     6481 atgatccggc tactacaaaa atggtgcagg tcaacaaagc agccactttg ttttttgggt
     6541 acagcgagga ggaaatgaaa ggcatgagtg taatggatct cgtctttaca gacgatgtaa
     6601 cgatggccag gcatatggcc gaagaagtac tggaagatac atccaactcg catgcggaat
     6661 tgctccgctt tcagaaaaaa acaggtgaga tcgtatatat ggaattatac ggcaccagta
     6721 ttaacctgga gggaaaatac ctgcgctctg ttattgcgat cgatattacc gagaaacttc
     6781 tttttgaaaa gaaaatgaca cgggcgatta taaaaacaca ggaagatgaa cggtatgaaa
     6841 taggtagtga actccacgat aacgtatgcc agatactggc ctcttcccag atgtatatga
     6901 gtatgctgga acctgaattg cagcaggaaa cggtcagtct gctcgagcag gccaaaggga
     6961 atacggggct ggcgatccgg gaaatcagaa acctttctca tcggctggca cccgcttttt
     7021 ttgatgaatc cagcctggaa gaatcaatgg aacggctgat taatgatttc tctatcgggg
     7081 acgaaatgca gattgattgt gtagtcagcg aacaggtact tgccctggat ctgggccatg
     7141 atctgcagtt aaatctttac cggatcttac aggaacaact ccggaatatt cagaaatacg
     7201 cccaggcttc caaagtggat atatgtgtgg atctggagaa cgggaaactg aatttgcaga
     7261 tcacagacaa tgggataggg tttgacttat cccgtgttaa aaaaggaatt ggaatcgcta
     7321 atatgaaaag aagggctgaa ttatttggcg gtaagaccgt tattttttct gaaccgggaa
     7381 aggggtgcca ggtactggtt accattcctc tgaataagca atcttcttaa gcactgtcga
     7441 ttgcccgtcc cggtcttacc gggtatagct attgccaatc cgtgtcatga tcattttggc
     7501 tgctgatgcc ggttagacta taatccggga atattgatta ggttgccagg tgtggatgac
     7561 tgcaaggcat gataagtccg gaaacagaat agcagcaagc aattattaca aaaggatcgt
     7621 aagaacaaaa tggccaccag gaatacaaca cgaaatgctg ggttaaccag gaaaaagaaa
     7681 cctgccggtc tgccttccgt tctgttatcg atcggatccg aacataaaag ttttcctttc
     7741 ctgaaacaac tcctgactcc gctccttgaa aacagtgatg ctgctttgtt gatcacgaac
     7801 ccgctgccgg aagcggttac tcttcgctta actgaatggc ttaaactttt taaattcaac
     7861 gtacggactg ctaaagacaa aacaaagacc agtgcccgta cactttattt aacgccccgt
     7921 ggcgttcgtt cttttatttt ggatgatgtg atccggttgg aagaactgaa tacaccaaag
     7981 gaggcgaaac aaaagcagga ccggctggat gctgttctgt atgacctgaa tgagcgcatc
     8041 aaagagcaag cctgtcttta tgggattacc aaactggctg ccaccgcacc gggactggat
     8101 gaattattac atggcgcggt caaactgatc ccgaagggtt ggcaatatcc gtcacatacc
     8161 tgttgttcca taatttataa caaagcggaa ttcagatcgg ccggtttcag gaaaacccgc
     8221 tggaatcagt tgattgaacg ggatgtacaa gccgggggaa agctcgttat tacggtttat
     8281 tacaaaaaga agatgccgga tgcggatgaa ggcccctttc tgaagtacga gcgatccctg
     8341 ctgaatagca ttgctgatag cctgatcctt tatatcagtc agctgcttgt taagagtcgt
     8401 tcccggcaaa gcgaatccat gttgaaggaa gcgcaggaac tggccttgct gggtaactgg
     8461 agttatgacc tgcttcagaa aaagagctat tggtccgatt cagtatatga tattttcggg
     8521 gtatcccgtc aacagatccg gcagtctttt ggggctttct tcaatcaggt ttcaaaagaa
     8581 gaccggaaaa gagtggagac aactatgcga aaagcccgca gggaaggggg cgactttagc
     8641 ctggactatt gtattcttac tccggccgga gagaaaaagt atattgaaga gtttggttac
     8701 agtgaaaagg ataagaaagg ccgggtgatt cgtcttttcg ggacggtaca ggatatttct
     8761 caacgaatga aggcagagca gaaattatcc gattcagaat acaagtaccg cagtctcttt
     8821 gaagcaagtc cggtacccag ttacattttt gatctacata cacaccggat actggatgtg
     8881 aacaaacgga tgacagagca atatgggtat acccgggatg aattactttc aatgactatt
     8941 ctggatattc gtccaaagga acaaataccc cggttacatg agcagcttga aaaagcaaac
     9001 cgggtgaaag gggttattca ttttggagta atgacccatc agaataaaga cgggagcctg
     9061 aagcaggtgg acatttccgg agaacagatc cgtatcgggg acagcgactg tatgctggtt
     9121 ctggcacatg atatctcaga aaaaatgatg caacaggaga tggatcacct ggaacggaat
     9181 attatagaga aatcgcttaa aagtaaaacg gctatcgata ttattttaaa ggattatctg
     9241 accggactcg aagaacttat aaccggatta agtgcctcga tcctacgtgt aagtgaagga
     9301 aaggtgtata actgggcatc cccttcgttg cctttatttt atgcagaggc attgaatgga
     9361 ttggcgattg gtccggaaga aggatcctgt ggtacagcag cgtataccgg aaaacagata
     9421 attgtatctg atatcaggaa aagtaattta tggaagaaat acaaaaaact ggccctgtca
     9481 gccggattaa gggcgtcgtg gtcgactcct gtttttaatc cggaaggaaa ggttatagcc
     9541 acttttgcaa attattataa ggagataaga atgccttctc cgtttgagct tgaaattttt
     9601 aaccgggctg cagtgcttat atctattctg attgaatcat atgaacagga tcaacgactt
     9661 cagcgcagtc atgagcgttt tggctttgtt acaaaagcta cttcggaagc aatctgggac
     9721 tggaatttag ttactgataa agtcttttgc ggagatggat ttaacaccat tctcggcgta
     9781 atactgaaag agcaggaatg gcgcctgagt gtttggctcg aatacataca cccggatgac
     9841 cgtatgttga ttacgcaaaa agtggaatcc gtattgcaag ggaaggagaa tcgctgggag
     9901 cttgaatacc gtattatccg gaaaaagggg cagctggtct atgtgtctga ccgtgcttat
     9961 attatacgga ataatcaggg ccggcccata cgtattatcg gagcgatccg ggatattaca
    10021 gtaaaaaggg aggaagaaca acagttgcgc ctgctcgaaa gtgtggtgac gaataccagc
    10081 gatgcagtgg tgattacccg ggcagatttg aatacggatc agggacctga aatcatttat
    10141 gtaaatgaag cattttcccg aatgaccggt tattcaccgg cagaagtgat aggaaaaacg
    10201 ccggctctgt tacaaggtcc taagtcggat tgggcggaaa tgaaccggct atcacttgca
    10261 ttaaaaagag gagaaccctg ttcggtcaat attattaatt atactaaatc aggaaaggaa
    10321 taccgggtgg aaatgatggt aaaacccgtg tttgatgcaa atcaccggat tacacatttt
    10381 tttgcgattc aacgggacat taccacacaa ttcaaactgc aacaacaacg acgcttcatg
    10441 gaaatggtaa gtagcacctt caaaaagatt gaaggcctgg ttatctccct tgatagtgta
    10501 ttgaagaaac tggtggagtt tggcgatttt acagcggccg aaacatggct gttgacagcc
    10561 gacaaacaac accttagcct ggtagccagt tttgcatcta ctaaagcgtc aaaggattac
    10621 tacgcagaga acaaagcatt gaatacgctc actcctgatg gtggattact gaaagaggtc
    10681 tgggctcgcc gtgaattggt gatcgtggat gactttgaca aacatgcgag tctgcgtcgc
    10741 cgggcagggg ttcagaaagc agggtttaaa tcagcgttag ccattccatt gatgaaccgt
    10801 ggagaactga tcggggtgct ggctgtactg actgataagc cgtcggctca tttgtcggag
    10861 tttgaacaat tgttcagggg actcgaagca gatctggggt ctgagatcaa acgtaaacaa
    10921 ctcgaaaatg aactgagttt aatttttaat acggcgcctg atattattgc cgttgtcgga
    10981 tttgatggtt atttcaaacg aatcaatcct gctgcttatg atatactggg ttattcgata
    11041 gaagaattat tgagtcgtcc atataatgaa tttattcacc aggattatat atctgccacc
    11101 aattcaatca cggcaacttt cccttcaatc tccggttcga attattttga gaattgctat
    11161 atcaccaaat ccgggaagtc ggtttggttg ggctggacct atcagcaaat accaggggag
    11221 gatctggtat ataccgtggc aaaggatatc accaggcaga aagaattgca gcgattactg
    11281 catgatgcca ccagtctggc ccgtattggc gcctgggaaa tggaacttcc tgccatgcag
    11341 atcatctggt cagatgtaac gcgtgagatt catgaagtgg aacccggtta ccagccggtg
    11401 ctgcaggaaa tttaccagtt ctatcaggat tcagataagt cccggctcga agaaagcgtg
    11461 ttaaaagcga tcggggaggg cattccctat gacctcgaac tcctgcatgt tacagccaaa
    11521 ggaaatcaac gttgggtacg tgttatcggt acacctgaaa tggccaatgg caaatgttta
    11581 aggatctatg gcagcttcca ggatattcat gcccggaaac tggccgaaat tgaattacag
    11641 caacggaccc gttatttatc cgccattgct acagtggcgc agatcttcct gagtaatgaa
    11701 aactggtttg atggaattaa gaattccttt gatctggcga ggtatacgat catggccgat
    11761 cagatttttt ataaggaaat ttatacggat aaagaaacgg gtcagtccat gtaccgccaa
    11821 cggatcggtt ggctgaaagg gaaggatatg ccagcgattg atccggtgga aacagaggtc
    11881 ttgcccattg attcattccc ggaagtaaaa gaggttttat tgtcggatca gccttatatg
    11941 gcagtgctta gcgaattgcc ggataacctc ctcagacagg tgttagttca ttcaggaatt
    12001 aaatctgttt tatttctccc ggtgtttgcc ggatctgtac ttgaaggggt ggtgggcgtg
    12061 gaggaatgtt accatgaaag acgatgggct gagggtgaaa tcttctttat gcggagtctg
    12121 tgttccagtt tgtcatctgc tattcaacgc ttcaatagca gtgctgcaca aaagaagctg
    12181 aatgaggatc tggtgaggca gacgagggaa ctaaaaatct ccaataccga actggaacag
    12241 tttgcttacg tggcttcgca tgacctgcag gaacctttac gcatgataac cagttttttg
    12301 accaaactgg aagatcgcta cgccatccgg ctggatgacc ggggtcgccg gtatatagat
    12361 tttgccgttg atggcgccaa ccgtatgcgt cagaatatcc tgggcctcct gcaatactca
    12421 agagtgggaa aaggcaatgc agctatcgag gaaattgacc tgaatatggt ggtagaggaa
    12481 atacgcctgt tgcaccagac agatatcaac gaaaccgggg ccgtcgttga agttgcggag
    12541 ctgccagtga ttaataatta tatggcaccc gtggtacagc ttatgaataa cctggtaagc
    12601 aatgcgctta aatacagaaa gccggatacg ccacccgtaa tacgaatttc tgccaggcca
    12661 gtggaggagt attgggaaat tgccattagt gataatggaa ttggtatcga aaaagaatac
    12721 ctgcagaaga tctttgtcat atttcaacga ctgcacccga aagaccagta cgagggtact
    12781 ggtatcggcc tggcagtggt taaaaaaatt gtggaaaatc tgggcggtaa ggtttgggtg
    12841 gaatccgatc cgggcatcgg cagtacattt tattttacca tccggtcaat taaggattga
    12901 tggagcgatt cgcgctactg ctgaaatcag ttaggccgac aactactaaa aagttattct
    12961 atggataaag atgaagaaat agccttatta aaggcacagt tacaacaatc aagggattca
    13021 ttcgaattgt ttgcttacgc tgtttcgcac gatatgcgtg aaccggtccg gatggtaaag
    13081 tcgtttatgg atctgttgct gaaaaagtac agtgattcgc tgaatgaaaa agctaaatca
    13141 tatgtcagtt atgcttctga tggggcggag cgggcggacc tgatgatcca ggatctgctt
    13201 ttttattacc gggccgttaa gaacattcaa cccaccacag cagatctcaa tatagcctat
    13261 gccggtgctt caggaaaatt aaaacgaaaa ctggaaaccc gtaattgtat gatcagttgc
    13321 cccgtactcc cggtagttca gggcgatgtg gatggaatac aggaagtatt tacttatgtg
    13381 atagaccatt tgctgtttca tgtaccttta gatgagcagc cccggctgca gatcggggta
    13441 aagcaggcag acaaaaaatg ggaaattacc tgtacgggta acgtacctga ccctgtaccc
    13501 ggtgaactgc gcgacctgtt tcgtttgttt cacaacaggt ctcagcaatt atcagcagac
    13561 agtaccctga ataaactggc tgttgcgaag cgtatcattg agtacctgaa cggggaaatg
    13621 aaggccgatc aggcagcagg gggtggtgtt gaatttaaaa tcacgctacc aatggttacg
    13681 gaataaacag gtggattaag aatgaaactc attagtgcat ataaacaaaa gatccgccag
    13741 aactgtattg attccggggc aggtaaaaca gatcgggttg aatactggca ggatacattg
    13801 ttttccgcga ccatggaaat aatgattccg ttgagcctga tagcagtgat accaggagtc
    13861 atcttttcat ttacgagcaa agtgccactt ttggccatcc tggatttggt agcctttcta
    13921 tggctttggc tggttggttt tgcccgcctg ccttctgtga ataccaggaa attccttttt
    13981 gttttctgcc tgtattatgt ggcctcatac atgttgctct atctcggcat taatggccct
    14041 ggactgcttt tcctgtttgc cgctgctata tttgcacttt tgatcttacc ggtcagctat
    14101 gcctggtggt ggagctgtat caacctgtca gcctgcctga tttttgcctt ggtactccat
    14161 tgggatatgg ccccgtttgc ggtggtgaat gaaataccgg tgagtgagtg gatggtggtt
    14221 tcttccaacc tggtcttcct gagtttcacc ttttccttcc tgctgccaaa actgatgaat
    14281 ggtttgtcag gcagtatgga gaaacaacgg ctgatgcaga tggatttgtt tgataaaaac
    14341 agtaacctgg aacaatatgc ctcgatcatt tcacatgaca tgcaaactcc tttgcggact
    14401 atctccggtt ttttaaacct gctagaaaag aaatacggaa acagcctgga tgataaaggc
    14461 aaagaatata ttcatttcgc ggtcgacggg tcagcccatt tgcaacatat aattaccggg
    14521 cttcttgatt tttcaagggc cggcaaatat gatgcaggta agtttgaaga attcagcctg
    14581 agagaagtag ctggtcaggt gattcagttg ctgaaagggc agcttgagga gaaggccggc
    14641 gaagtccgtt gcgagtcaga gttaatgctg ttttctaacc gatcggcttt tatgcaggta
    14701 ctccataacc tggtagggaa tgcaataaaa tatagccgta aggatgtgcc accccggatc
    14761 agtatcagtg tacaggatgg aattgcagac tggactataa aagtggagga caacggaata
    14821 ggtattgaag aagaatattt tgaaaaaata tttattattt ttcagcgact gcattcccgt
    14881 atggagtatg aaggaacggg aatcggactc gcgattgtaa aaagaattat agaaaaactc
    14941 aacgggcgta tccgggtgga atccaccccg ggtgccggaa gtagttttta ttttaccatt
    15001 ccaaaacaga atgattaatt atgaaagaag tacatatttt actggtagag gataatgaag
    15061 gggatattgt cctgacactg gaagcctttg aagaaagtaa aatcctcaat aaattcagcg
    15121 tggtgaggaa tggcaaagat gccctcgact ttttattcaa acaagccccc tacgaggatg
    15181 ccaaagaacc ggatattata ctgctggata tcaatctgcc acttaaaagc ggtattgaag
    15241 tactgcagga agtaaagaat gacgaccggg tgaggcatat cccggtaatc atgctgacca
    15301 cttcatcctc tgaaaaggat atttcccttt catataaaca ccatgccaat tgctatatca
    15361 ccaaaccggt agaggtattc aaattcctcg aagccgtgaa tgccattgag catttttggt
    15421 tacagatagt gtcactggac gggaaaaata aaagggaata atggtgcgca gacgccgatc
    15481 agataatccg gaaacctttt ttcgaaagtt tctcatacct ggtcttttca aaaacaaaaa
    15541 gaaatgcccc tttcctggat gattcctttt ccttctcttc cagttttttc agaataccga
    15601 gttcgaggat ccgccgggtg aaattgcgct tatccatttc tttttcataa atggcttcgt
    15661 aaagtccctg caactgctgc agggtgaatt tgcggggcag cagggtaaag ccggccggat
    15721 gcgtggatac tttatctttt aatttttcct tggcctgact gatcatctcc ccgtgatcga
    15781 atatgagcgc gggaagatga tccagttcaa accatttgga attatgttct ttcatcagtg
    15841 actcactata gtcattgatc tttatcaggg caaaaaaggc aatagacacc acacggccac
    15901 cgggatctcg gtttacattc ccgaaacaac ccagttcctc catatataca tcggtcagac
    15961 cggtcagcag gttcagcacc cgggtagctg cttctttcac actctccgtt tttttcacaa
    16021 aaccgcccat gagtgaccac ttgccgagtc cgggttcaag attccgtttg accaacaggg
    16081 ccttcagccg gctgccgtca aaaccgaaaa tgatacagtc cacagcgagc agtaaatgat
    16141 cctctttttt atataaatca atggccataa tcctgctaat ttccggttac cagaatttaa
    16201 tatataaagc agccaggatc ataatcgtgg tcacaatcat gatcaggata ccgggcttca
    16261 ctttaaacat ctcactgtca agttcaaagg ctttgggatt tactttcggt ccgcccaggc
    16321 tgataccgat catgaccacc atcgtaaaga agaaggccca tcccatattg atcaggaacg
    16381 gaatttcata aataccatct ttgtttttga aagcggtata taatattgtc tcattaccca
    16441 ttaatggaac cgcaaaacta ttgaagaaga tagccagtaa gaatccggtg agcagaccgg
    16501 ctactgcagc cgtacccgtt gtgcgtttcc agaacatacc caggataaac atggcaaata
    16561 cacccggact gataaagccg gtgtacttct gtataaacgt aaatccacct tcgccactga
    16621 tacccaacat gtctttccac tcaaaaatga cggcgattgc aatagagacc agggcagcca
    16681 tccggcctac ccaaaccatg ttcttctcct tgctctcatt actttccact ttctcctttt
    16741 tgaaatattt catataaata tccagcgtga aaatggttcc gatactgttc agtttacccg
    16801 ccagtgaggc tacaatggca gcggtcaggg ctgcaatggc cagtcccttt aaaccggatg
    16861 gcaggaatcc gagaatagct gagtaagcat catccatacc acgcagactt tctaaatgac
    16921 cattctgatg caatacataa gcggcaatac cgggcagcat cacaatgatc ggcattaaaa
    16981 gtttcaggaa accagcaaac aggatcccct tacgggcggt ttccaggtca gcacccagtg
    17041 cacgctgcgt gatatactgg ttacaacccc agtagttcag gttcacgatc cactgaccgg
    17101 caaaatacat ggcgataccg ggaaggatca ggtatttatt gacgtcttcc tgggaagaag
    17161 cagcagtagg tttctgcagg atcatatgga aatgatccgg cgcctgctgc atcagggttt
    17221 tgaaacccgc aacagcatca ctgcccagtc cgaatttttc actgacaatg gtcagagcga
    17281 aatagatggt ggcaaaacca ccgatgatca gtacggctac ctggatcaca tccgtatacc
    17341 cgatcacttt cataccacca agggtgatga tcagggagaa tacggccagc cccaccatga
    17401 ccacgtgcag gtattcaggt ccgatcagtc cgctgatggc aatcgcaccg agataaagaa
    17461 tagacgtcag attgacaaac acataaagca ataaccagaa gatcgccatg atcagtgata
    17521 cactttcatt ataccgccgt ttcaggaact gcggcatggt atagatcttg ttcttcaggt
    17581 aaaccggcat aaaccagacc gcgatgatga tcagggccag cgccgcgatc cactcatagg
    17641 ccgcaaccgc gatccctacg aaataaccgt taccactcat tccgataaac tgttcggctg
    17701 agatattaga ggcaatcagg gaagccccga tagcccacca ggtcagcgat ccttcggcca
    17761 ggaagtaatc gtgtgaggca gttacagttg ctttcttttt tttgttatac acccagtaac
    17821 cataaccggc tacgaggatg aaataaatga cgaaaatgag aatatcaagg gttgaaatac
    17881 cactcatgtc aattgatttt tggttgttta tttaaaagag gcgtcgttcc agcgcaattc
    17941 gttcttgaat tgataaagat tcgtgttttt accgataagg acaaattcaa ttccggccat
    18001 ttcagcaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctccagcat
    18121 atgtgagccg aggactaaat tattctttgg ttcaaaatga taagtataat cttccatgaa
    18181 cgaatttcca ccgggtaaac cactgcccat tactttcatg gcacgtacca gtgcagctgt
    18241 tttccagtca ccttcaccac caaagccata gccttctgcc atcaggcgtt gtgatgcaat
    18301 accgggtaat tgtttcatcc catgcaggtc ttcaaacgta tcggtatagc ccttgaagcc
    18361 accctgctca aggaaggtcc gcatgccgat ttcaatttta gccgcatccc ttaatgactc
    18421 atattgtttg ccgcctttct tcaaagaagg cgccagttta tattctttat tgtatactgc
    18481 aatgagctca tcaatcgccg cattctttac tttattgatt acggccacca ggtcacctac
    18541 gccgtaggta ttcacactgt aaccgaattt tatttcggct tccactttat cgccttcggt
    18601 tactgctacc tgtcgcatat tatcaccaaa gcggcagaaa cgggcgccct gccagtcctg
    18661 ccagccggca gccgcacggg cccaactatt aattttcttt tgtacctccg gatcctgcca
    18721 gtgaccagcc accactttac ggttcatccg catccggctc atgataaagc caaactccct
    18781 gtctccatgc gcactctggt tcaggttcat aaagtccata tcaatgctac tccagggaat
    18841 atcgcggttg aattgtgtat gcaggtgcaa catcggtttg cgcaggatct tcagtccgtt
    18901 gatccacatc ttagccggag aaaaagtatg catccaggtg atgatcccga tacaactatt
    18961 gtcattgttg gctgcattga tcaggttgaa tatctcttcc ggtgttttta caacgggttt
    19021 gaaaatcacc tgtacaggaa tatgtttagc ggcattaagg gtgtctgcga tcttcgcaga
    19081 atgttcatcc acctgtttca gggtttcttc tccgtacaga tgctgactgc cggttacaaa
    19141 ccagacagac cattttttta aatcaggtaa ttgcataaag agtgttttga caactatggt
    19201 tgagtggaaa atttatagac acaggtatgg tgataggtct caccgggttt taaaatcgtg
    19261 tttgggaacg aaggttgatt cgggctgtcc gggaaatgct gggtttccag gcagagagca
    19321 ccatgccaaa catattttac accgcctttt gtattggaga gtgtgccatc caggaaatta
    19381 ccggagtaga actgaagtcc gggttcagtg gtgaatactt ccattacacg gccactgccg
    19441 ggatgtacca gtttcgcagc cagttccaat ccggttccac tgcgggtcaa tacccagttg
    19501 tgatcatagc caccggttat tttatcaata tcacgtccaa tgggtttggc ggtattaaag
    19561 tccatcgggc caccggctac cgcttcaatt ttaccggtag gaatcaaggt agaatcaacc
    19621 ggtgtatatt gagcagcgtt catcatcagt tcatgaccga gtatggtgct gtcagctccg
    19681 gcgcttaaat taaaataagc atgactggtc aggtttaccg gggtggcttt atcggtggtg
    19741 gcgctgtagt cgatcttcag ttcattggct gatgtaagtg tataaatcac ttccattttt
    19801 aaagtaccgg gatacccttc ttccccgtcc ggactggtat aagtcagttt caggctgctg
    19861 tcgccggcct gcttctcgat tgtccagtat actttatcgt accctttact accaccatgc
    19921 aaggcattaa caccattgtt ggcagccagg gtatattttt taccatcaag cgtgaaaccg
    19981 gcttttgcaa tacggttacc aaagcggcca atcaatccgc cgaaataggg attgcctttt
    20041 tgctgaaacc cttccaggtt agcgaaacca aaaaccacat cccccatctt gcctttgtta
    20101 tccggcgtga tgagtttact gatcgctccg ccatagttaa tgatgccaac ctgcataccg
    20161 gatgcgttgg taagtgtata ttccgttacc ggcttaccct cgaatgtgcc gaatggcatt
    20221 tcaccaatac ccggcttggc gtttaactga gcggtaccgt ctttttgctc tttttcagta
    20281 ctgttattac aggcagccag ttgcagggca agggc
//
LOCUS       DAOPMW010000022        19697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2638_length_19697_cov_8.280165, whole genome shotgun sequence.
ACCESSION   DAOPMW010000022 DAOPMW010000000
VERSION     DAOPMW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_19697_cov_8.280165"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2581
                     /gene="gcvP"
                     /locus_tag="PLU11_02280"
     CDS             <1..2581
                     /gene="gcvP"
                     /locus_tag="PLU11_02280"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPN57963.1"
                     /translation="RNIFENPGWYTQYTPYQAEISQGRLESLLNYQTMVSDLTALPIA
                     NASLLDEATAAAEAMTMFFNALNKQDHIERPRFFVDAELFPQTIDVLKTRAVPVGIEV
                     VTGDYKTASIDATYFGAIVQYPNDKGSVEDYRGFISQVHAAGAFVVMATDLLALTLLT
                     PPGELGADAAVGSAQRFGVPLGFGGPHAAFFATKDEFKRNMPGRIIGISIDAQGNRAL
                     RMALQTREQHIKREKATSNICTAQALLANMAAMYAVYHGPDGLKNIAKRIALYTQTVA
                     EAIQERGFDLVSDAFFDTLVVKTNDLTIIREKAERQQINLRYIDAHHIGISVDETTDV
                     ADLFDLINCFENDKDPVAFTIDQEGELDHIPTSLTRTSNFLTHPVFNTHKSESQMMRY
                     IKYLENKDLSLNTSMISLGSCTMKLNAASEMMPLSWAHWSKVHPFAPSAQTEGYMYMA
                     NELSKYLCEITAFDACSLQPNSGAQGEYAGLLTIRSFHEANGDHHRNVMLIPISAHGT
                     NPASAVMAGMKVVVVKALENGYIDVDDLKAKADQYSKELAGIMITYPSTYGVYEETVK
                     DITDIIHQHGGQVYMDGANMNAQVGLTAPGLIGADVCHLNLHKTFAIPHGGGGPGMGP
                     ICVKAHLAKHLPGHVHEGTAHAVSAAPIGSASILLISYGYIRMLGAEGVRAATEYAIL
                     NANYMRARLAGNYDILYTNNNGQCAHEFIVDLRPFKKSAEIEAEDVAKRLIDYGFHAP
                     TMSFPVAGTIMIEPTESEDKAELDRFCDALLSIREEIRAVEEAKADKKDNPLKNAPHT
                     QFVLTADEWKHGYTRQEAAFPLPYVSANKFWPSVARVNNTHGDRNLICTCEPVESYMI
                     AEA"
     gene            complement(3910..5316)
                     /locus_tag="PLU11_02285"
     CDS             complement(3910..5316)
                     /locus_tag="PLU11_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN57964.1"
                     /translation="MKKTILLFSFIATVQIVFAQKSNVDTILQKVALEKDENKRFDLY
                     ISMVGAEINNDPKWCIETGLKILNQAQRGNDKIGQTMAYSFLGQGYRLLGNSIKALDF
                     HHKAIAIAEKTSNQSLLASAKNQTGHIYRDREEYDKAAKIYLSSTAHADKGHNEKMKG
                     WAPSNLGAVFLATNNLDSSLMYSQRAYEIFTRLKVISNNPFVFVNLGGVHSKMGNTPV
                     AMGYFNMALNRSEGTSNIRYRNLTCTALAEHFQRNNQTDSCVFYARKAIETVSNTTFF
                     YLSSKPAKLLSDIYEKTNCDSTLKYAKLIKTANDSLSSNKTNQQILLMTFEEDLRQQE
                     LVTEKIKEQQQRKQNIQYALLALGIITFIIAFLLLSRKLITNTKLIQFLGVVALLLVF
                     EFLNLLLHPFLERTTHHSPALMLLALVCIAALLVPLHHKLEHWATTKLVEKNKKIRLA
                     EAKKTIQELETNPDDKSE"
     gene            complement(5446..5862)
                     /locus_tag="PLU11_02290"
     CDS             complement(5446..5862)
                     /locus_tag="PLU11_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57965.1"
                     /translation="MSACTGNKETNSLNQLVSQKTNSLKILQNKIDSLRNESIELKEE
                     LNEWFTEIEIDKLRAKGIQHPEQEITIDLLNKPNLIPYLGVLGGTMRFGDIKLLGDRW
                     VVAYFADGHIDGEMILRYTITKDLKIKWTVIDHYLN"
     gene            complement(6172..6963)
                     /locus_tag="PLU11_02295"
     CDS             complement(6172..6963)
                     /locus_tag="PLU11_02295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPN57966.1"
                     /translation="MHDFRSSGLLKVPGFWILFIFLLFIVGRFIKPAFPASWEQFVYG
                     IGGTIGTFLLLWGFIKAEKGTFRNYELFWQSGTLPRFFKGFLYGAIIFSCIMLVLVAA
                     GGIRIRFSNSWDTSAAFWYLSIIPLALFEELAFRSYPFILLKKKFSFLITQLIVAVAF
                     AVYHMIMGWDTTVAILGPGIWALVFGLGAAWSKGIALPTGIHVALNFSQQVVGMKEDN
                     GGNIFIMQELPSAAFIPANTVGMICQVAVGVLALILTVIYRDRLK"
     gene            7137..8177
                     /locus_tag="PLU11_02300"
     CDS             7137..8177
                     /locus_tag="PLU11_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN57967.1"
                     /translation="MRQTDSDIIIIGAGGIVQHAHLPAYKKAGYTVRGIYDKDIAKAK
                     IVAADFGLPVAFASLEQAIDAAGRSTVFDLAIPAAALLSVLEKIPPGSSVLLQKPMGE
                     NLEQAKNILKVCNEKKLSAGINFQLRYAPFILKAKELLNSGQIGTLCDIEININVLTP
                     WQLWDFLFTLPRMEILYHSIHYMDLVRQLAGEPESVFARTIKHPDMPQLASVKSMIYM
                     DYGENLRASILTNHCHKFGPRHQHSYIRIEGTRGAIYIRLGLLMDYPQGVPDEFEYIT
                     LDENGEGKWKSISIEGSWFPDAFAGSMGEMMKAQSDKKYKPDNNLEDGLKTMRWVERA
                     YQSTENGKSVKK"
     gene            complement(8239..11037)
                     /locus_tag="PLU11_02305"
     CDS             complement(8239..11037)
                     /locus_tag="PLU11_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57968.1"
                     /translation="MKKNLFFSLFFLCIVSQYSRAQGGSKETVLSNIRSRLVSFTGAV
                     SLNDADFTILSEDEKGLRLTGTLSVFSLSNVKADISATSNSATFTAGFSNGVTSQLTV
                     DGKKLSSWLPSWLNGKMDLLKLSLSYQDAGASEAKMMVELGPPAAATPIIFQGMQLSQ
                     PSFTLILDKTGTNTITQANFSSALKFGLLNLSLSGTAASDAKWSFTANIGSLSLKDML
                     SSMASAVSVSVPVLPAPFENFTLNKAFLSFNSDNQINLAGAWNMGSMEAFFDQPKGQM
                     VLALSPDNSFSFAKVSEILAPMDQIGFKDMAIIVANKEIVVKKMTGVLHKLNLDGSNI
                     PAGVQVMGEFRVPSDLPGFRAKTTLTLSGLFPVPLALPTVKAATTFKNMEFGKRFNLL
                     ESFLQLNPADMKFGAGLRFSYKPDNQKSLDFTGMGEVSPPATFNLITYMNKGSFWNEP
                     FGIKGLRIGELGLDIGADVLSPVPRPVIGIAGTIKIGSFSGTGSGSLNTANPLESAVS
                     LSVNEISFQQVVNELMSDAVKAEINKLPVGARDFKITNAVLSLVPKTTTIAGKTLDQG
                     FQLSGTGYISGFRARLNMGGSNSNGLFGEAVAAPVIVKNGSTEIFKLTGANGKDSSRI
                     MVDLTKTTLAGGNPFMFFDATASLLGMTAGVNVKISGTGFKFTTTGKLFNKFSADLDG
                     SGSGSIDHSPGMEIRAAMKSDLFEILNTEILSFINKETKTDQDRYKKAKDDLKESRDY
                     LARTKADVEAFENEKERVEKQRKKVRRLKNEAEDINLNNEPWRGPEKAALWAAYETAN
                     GILKGYVKTLEKLSEAINWTKQNAYKDLVNSSLSVINGFETFTTGTKAAARWVVDNGL
                     ENMVDVKSAEFKGKLNVVSGGSVTMKTNVTYMDNDYNLSYEFEFNDPAEAAERIARKL
                     LDNLASSGSSPEFGKELF"
     gene            complement(11120..11752)
                     /locus_tag="PLU11_02310"
     CDS             complement(11120..11752)
                     /locus_tag="PLU11_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN57969.1"
                     /translation="MIEVAIIEDVEAIRKSITAYLDAQEEFAVTASSNSVEAFLALDP
                     AEIKPDVILSDIGLPGMNGIEGIRLFQKKFPDAEIIMLTVFQDEEHIFKALCAGATGY
                     LLKSTPLPRIRESILDITVGEVPMSKAVARKVLEHFKPNRTYGVKMDELTAREKQVLQ
                     NMVEGLSYKMIAARLDTSLETVRTQVKSIYKKLHVNSKAEAIRIAMHNKM"
     gene            complement(11812..14868)
                     /locus_tag="PLU11_02315"
     CDS             complement(11812..14868)
                     /locus_tag="PLU11_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN57970.1"
                     /translation="MKGRILFLWLYFTSNLAVAQAPGLTVDFISTEQGLPHKLVNCIT
                     QDNRGFMWVGTANGLFRYDGYSFYDYTKHVSGSDQLKGLNIGSLLKDKEGNIWIGYND
                     GLSKMDPVTGKTSRIPLTGYTGTEQRVKRIMLDSQNRILIYLADNTLLCYDSHFKLRF
                     IYHFSIRVREDGLDHPVILEDADHSFWLPTFFNGIDHISAEGRFLEHFRQVKDAGKNW
                     PVKVLMDPYGIEYKFALGPRFDKVIQHPALVVNAAQHVLLRPYASPSGILDQAGNEWN
                     IKWNTVHLTDPVAKKELEFTLSYSNDFFYDYPWLNVYQGSDGIIWTCSRKGIFKIHYR
                     RSPFVNFLYSTPSPQKAGISIRGITEDKSGDIWIGSYPYAANSTVNNTVLSVIRASNK
                     NTAKPFLFYQPDGGAYDVGGSPVYRMHDEEEYLLASSEANYMLHIEKRTGRVSKDTCP
                     FEVLAPIKAFIYRNDSTVFVTSGTAIGIYRKRPGQKARFRLLYKNKEGDPFDGVNNFF
                     DAGNGKCWAVGNDGLYLLNDEGVLLRSFSMGNVPSLLLPVLDINYLYREDAHLFWLGT
                     RHGMIRLDTLKKNSRVYTTNDGLPNNNIMSIIPDEFGFLWLSTDNGLSRFNPAQEIFI
                     NYDVNDELPHNEFNRMASLKASDGRIYFGGLNGVTAVYPRDVDTSLSNLQPQLVSYSK
                     YDGKRDSVFIFQASEVSENRIIFSHNDRLFNFRVMLPAYRNTSKNRFLYKLQGWDKEW
                     QSAPNTNMISYSFLPAGDYELVVKGASAGDTWARNEYRLKIKVLVAWYKTWWFYVLLA
                     AVLGLLVYTFYRYRINQVLKLEKIRNKISADLHDELGSVLTQISLQSEMVSSNIYDEE
                     EKKNELKNISNTSRRAIHAMSDIVWSIKAGHDKTSSLLDRMRDHADLMLQPLNTEVDF
                     TVTGIDEQKEMDNTVRQELFLIYKEAIHNIVKHSQPSGVQIRLENGKEGFEMEITNDI
                     NQLQSYGVLGGNGLKNMQERALGIRARLEIKKEENRFTLTVRREWI"
     gene            complement(15233..16687)
                     /locus_tag="PLU11_02320"
     CDS             complement(15233..16687)
                     /locus_tag="PLU11_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57971.1"
                     /translation="MKRTFTLPALVLFIVLGSSWLQSAVAQYNQNDSYTQDDQYSPND
                     RNREVSYQDFYDQLSPYGQWVDHPDHGYIWIPDAGPDFRPYSTEGHWVWTEDAEWMWV
                     SDYDWGWAPFHYGRWDQDQHYGWYWVPGYEWSPAWVAWRDGGDYYGWAPIRPGISINV
                     NYNMGSYAPPYDFWSFTPRRYILYPRVRDYCVDYRRNSSIFSVTTVINFNIGGRWGYR
                     SGPSRWEAERYCGRITPVRFRDSYSPGRTYFRNNEVRIYRPNVRRDEGRGYAPQRFER
                     YDRNRDFRTAPRNNGNGRINNGGANGRPENGNRNNGFGRNENGNRDNGRINDTRGGRP
                     VNRDFRTNGNGDRQPQSGGTPNNNTGGRRFERNDNNSNNGGNNPRMGGSNNPRTGPGA
                     DRPQRRPDNGNSGNTEPRRFERRNDNGGGAVQGNNNRSIERRPVSSGSNGQQRMERRT
                     APQSQPAQRQVERRSENSGGNGGGNSGGNGRRRF"
     gene            17442..18293
                     /locus_tag="PLU11_02325"
     CDS             17442..18293
                     /locus_tag="PLU11_02325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPN57972.1"
                     /translation="MEQRFINLYDEYTHKPLPRQEFIKRLTMMAGSTAAALALLPLLE
                     VNGAHSAITPQDDLFTERISYAGVNGDMQAYVARPKDDKKYPAVVVIHENRGLNAHIE
                     DVARRAAKAGFLAIAPNALSSLGGTPENADEARTKFQQLKPEDNLQNFIKVFDYLGTR
                     KDANGSYGCVGFCWGGAMSNNLAVNVPSLKAAVAFYGRQPATEDVAKIKAAVQLHYGS
                     MDEGVNKGIAAYEEALKTNKIAYELYMYEGAQHAFHNDTAPTRYNEAAAKLAWERTMS
                     FFQKQLK"
     gene            complement(18450..>19697)
                     /locus_tag="PLU11_02330"
     CDS             complement(18450..>19697)
                     /locus_tag="PLU11_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57973.1"
                     /translation="NMMGDLRGVLTNGAERGLSTFGMIGVEMTGGTGQALKVAPGKTA
                     ELSFPIPASLQAAAPDSIDLWHFDEATARWKQEGKAGKTGAMYVAKVSHFSFWNCDAP
                     FPLIDLCMSFKDDHGAPLNNVQVRIKRIVNGSYGYGRTDSTGSLCGKVPKNENLELQL
                     LDICGNPFYTQAIGPFTTNSTLPVITVTLPAATSLTISGTLTTCATTNVVNGAAAIYI
                     EGGHQYTVAVTNGVFSVTIPRCTNTAINYTVLGVDYATLQQSIPVSGTGTTGTVNVGT
                     LQACGTSSLEYIEVMIDGTPFTLATPADYINYNDSTGAAPYLFNTWISGARQNSGTTS
                     GGIQFSFRHNQSVATGLPLDFVYVSAGPGMTNSQSFAAGAASVNTTVFGAAPGGFVEG
                     NFTVQMNLGGTPRTVVCNFRVRR"
BASE COUNT         5409 a         4772 c         4212 g         5304 t
ORIGIN      
        1 ccggaatatt tttgaaaatc cgggatggta tacccagtat accccttacc aggccgagat
       61 cagccagggc cgcctcgaaa gcctgctgaa ttaccagacc atggtcagcg acctgaccgc
      121 tttacctatt gccaatgctt ccctgctgga tgaagccacc gcagcagcag aagccatgac
      181 catgttcttt aatgccctga acaaacagga tcatatcgaa cgtccgcgat tctttgtaga
      241 tgctgaacta tttccgcaga ccatcgatgt actgaaaacc cgggcggtgc cggtgggtat
      301 tgaagtggtg accggagatt acaaaaccgc ttcaattgat gccacttact ttggtgccat
      361 cgtccaatac ccgaatgata aaggttcggt ggaagactac cgtggtttta tcagccaggt
      421 acatgctgcc ggcgcctttg tggtcatggc caccgacctg ctggccctga ccctgctcac
      481 tcctcccggc gaactgggtg cagatgcagc tgttggttct gcccaacgtt ttggggttcc
      541 cctgggattt ggcggaccac atgccgcgtt ctttgccacc aaagatgaat tcaaacgcaa
      601 tatgcccggc cggatcattg gtatcagtat cgatgcccag ggcaatcgtg ccttacgtat
      661 ggcattgcag acccgcgaac aacatatcaa acgcgaaaaa gcaacttcga atatctgtac
      721 cgcacaggcc ctcctggcca atatggcggc catgtatgcc gtatatcacg gaccggatgg
      781 attaaagaat atcgctaaac gcatcgccct ttatacccag accgtagcgg aagccatcca
      841 ggaaagagga tttgacctgg tttccgatgc tttctttgat acgcttgtgg taaaaaccaa
      901 tgatctgact atcatccgtg aaaaagcgga gcgccagcaa atcaatctcc gctatatcga
      961 tgcacatcat atcggtatct ccgtggacga aaccaccgat gtggcggacc tgtttgacct
     1021 gatcaattgc ttcgaaaacg ataaagaccc cgttgccttt accatcgacc aggaaggtga
     1081 actggatcat atcccgactt ccttaacccg tacgtcgaat ttcctgactc atccggtttt
     1141 taatacgcat aagagcgaaa gccagatgat gcggtatatc aaatacctgg agaacaagga
     1201 tctttcattg aacacatcga tgatctcact cggcagttgt acgatgaaac tgaatgccgc
     1261 cagtgaaatg atgcccctga gctgggccca ttggagcaaa gtgcatccgt ttgccccgtc
     1321 tgctcagaca gaaggttaca tgtacatggc caatgaactg agcaaatatc tctgcgagat
     1381 caccgcgttt gacgcctgca gtctgcaacc caacagtggt gcgcaaggag aatatgctgg
     1441 attgctcacc atccgttctt tccatgaagc caatggcgat catcaccgca atgtgatgct
     1501 gatcccgatc tctgcacacg gcaccaatcc tgcttctgcg gtaatggccg gaatgaaagt
     1561 ggtggtggtg aaagccctgg agaatggata cattgatgta gatgacctga aagccaaagc
     1621 cgatcagtac agtaaggaac tggcgggtat catgatcacg taccccagta cctatggcgt
     1681 gtacgaagaa acagtaaaag acatcaccga tattatccac cagcatggcg gacaggtata
     1741 tatggatggt gccaatatga atgcacaggt aggcttaaca gcaccgggtc tgatcggagc
     1801 ggatgtatgt cacctcaacc tgcacaagac cttcgccatt ccacatggcg gtggcggtcc
     1861 cggcatgggc cccatttgtg taaaagcgca cctggctaaa cacctgcccg gtcacgtaca
     1921 cgaaggaacg gctcatgcgg tatctgcagc accaattggt tctgcttcta tcctgctcat
     1981 ctcctacggt tatatccgta tgcttggcgc tgaaggtgta agagcggcta ctgaatatgc
     2041 aatcctgaat gcaaactata tgcgggcaag actggccggc aattatgata tcctgtatac
     2101 aaataataac ggtcagtgtg cacacgagtt catcgttgat cttcgtccct tcaaaaagtc
     2161 agccgagatc gaagcggaag atgtagccaa gcgcctgatc gattatggct tccatgcgcc
     2221 caccatgagt ttccctgtgg ctggcactat catgattgag cccacagaaa gtgaggacaa
     2281 agccgaactg gatcgttttt gcgacgcctt attatctatc cgtgaagaaa tcagggcagt
     2341 ggaagaagcc aaagcggata aaaaagacaa cccgctgaaa aatgcaccgc atacgcagtt
     2401 tgtactcacg gcggatgaat ggaagcatgg gtacacacgc caggaagcag ccttcccgct
     2461 gccctatgtg tcagccaata aattctggcc atctgtagcc cgggtgaaca acacacatgg
     2521 cgaccggaat ctgatttgta cctgtgagcc ggtggagagt tatatgattg ctgaagctta
     2581 ggagaaagta ggcagtaggc agtaggcagt tggcagttgg cagaatgtcc ttgtcatcct
     2641 gcctgtcccg ccaaatgcgg taagcctgcc taccggacag gcaggttttt agaagattaa
     2701 tagcctaaaa agcaaattac cagcataaaa tagaaagccc cttctgtatc aagttaacag
     2761 aaggggcttt ttatttctgt gtataaaaaa acgtttaaaa gggcatttcc ggcggcaata
     2821 aataacagct tgtatcaacc caaatactgt tcagctaaac ccaaaaaaag ttcggttttt
     2881 gcttgatttt ttacacagtt catgtttggc gctggccaat tcattcaccg tgacggaaag
     2941 ttcagtcctt actatttttc catatctatc cgcgaccacg agtcgaagtc gatgtagaca
     3001 aaatttgtca ccgtattgtt tttgaactta gtttttttgt atcttttatc aaccaatacg
     3061 cattgaacaa caacaatgga accaaccaca caaataggtc tgaagccaga aatgccgttg
     3121 cttcatttgg agatatataa taaaatacgt ccatagcgaa agtgtcaatt acccttatag
     3181 aaacaaagta ggccgcgcag ataaaactgc ttaccatcat tcgtttatgc atcacaactt
     3241 ttctttgctt tataaaataa taagcaagca ttgtaaatag caaccataaa cttgttacgg
     3301 taaattgtga aggccgacac gcaatgcacc aagttcgggg tatcataata tacaacgttg
     3361 taatacataa caaggacgaa agaatataaa gcttgcccgt agttcgatga aaggaagtgt
     3421 atttgttgcg tattgcaggt gtgaattgca atacagcggt gctataaaca atgatgccgg
     3481 caaaaatatg taatgcaaaa atactgttgt caagcgtagt gttacttccc caatgttcta
     3541 aagtgtcatt gctgaggtac ttgtacttag gaatcaattt gtataaaaag aaaccgaaga
     3601 agacaagcca aaaaaaggca attgaaaata ttttcaacat ttattgatat tcagttttgg
     3661 gattagttta gtatttcatt tcgagcgtcc aacgtatggg gctttgtgca gtggccgatc
     3721 cgatcctgcc tgccgtcagg catggatcct ggcagcgggg ctgcaaaaag ccgttcctgg
     3781 gttaaattct tttttagatc aaatttctat gaaaccatgc cagcccagta tgtcgctggg
     3841 tattattctg tcgatgagaa tttattctga cggccattgc acaaaatccg gtgttgagcg
     3901 ttcgtgcttc tattctgatt tgtcatcggg gtttgtttct aactcctgaa tggttttttt
     3961 tgcttctgcc aatcttattt ttttattctt ttcaaccagt tttgtcgtag cccaatgttc
     4021 caatttatga tgcaggggca caagcaacgc agcaatacat accaatgcca gcaacatgag
     4081 tgccggagaa tgatgggtgg tgcgttctaa aaagggatga agtaataaat tcagaaattc
     4141 aaataccaat aacagcgcga ccacgccgag aaattgaatg agctttgtat tggtgattaa
     4201 tttccggcta agcagtaaga atgcaatgat gaaagtgatg atgccaaggg ctaacaaagc
     4261 gtactggatg ttttgttttc gttgttgttg ctcctttatt ttttctgtaa ccagttcctg
     4321 ttgccgtaaa tcctcttcaa acgtcattaa taaaatttgc tgattcgttt tgttactgct
     4381 taacgaatcg ttggctgttt ttataagctt tgcatatttt aatgtgctgt cgcaatttgt
     4441 tttttcatag atatcagaaa gtagttttgc cggtttactg ctcaggtaaa aaaaggtagt
     4501 attgcttacc gtttcaatcg cttttctggc atagaagacg caggagtcgg tttgattatt
     4561 tcgttggaaa tgttctgcta aagctgtgca agttaaattg cgatacctga tattagaggt
     4621 gccttcactt cgattgagcg ccatattaaa atagcccata gctacggggg tattgcccat
     4681 tttactatga acaccaccca ggtttacaaa tacaaaagga ttattggaaa ttacttttaa
     4741 ccgggtaaaa atttcataag ccctttgtga atacatcagc gaagaatcaa ggttattggt
     4801 agcaagaaat acggcaccca ggttcgaagg agcccagcct ttcatttttt cattgtgtcc
     4861 tttatccgca tgtgctgtgg atgacagata aattttagca gccttatcgt attcctccct
     4921 gtctctgtaa atatgccctg tttgattttt tgcggaagct aataaagatt gattactggt
     4981 tttttctgcg atggcaattg ctttatgatg aaaatcaagg gctttaatag agtttcccag
     5041 tagccggtat ccctgcccca aaaaggaata ggccattgtc tgtccaattt tatcattgcc
     5101 tctttgtgcc tggtttaata tctttagtcc ggtctcaatg caccatttcg ggtcattgtt
     5161 aatttctgct cccaccatgc ttatgtataa gtcaaatctt ttgttttcgt ctttttctaa
     5221 cgcgactttt tgaagtatgg tgtctacgtt tgatttttgt gcaaaaacta tttgcacagt
     5281 tgcaataaaa gaaaagagga gtatcgtttt tttcattttc agttgaagta gctaaatatt
     5341 gaatctagca atttaggcaa tatctcgcca aaatatatta ataaattatg tgttgagtat
     5401 atctactgat ggccattgca caaaaccaaa tgttgtacgc aattgctagt tcaaataatg
     5461 gtcaatcact gtccatttaa tttttaaatc tttcgtaatg gtgtatcgta atatcatttc
     5521 tccatcaata tggccatcgg caaagtatgc aactacccat cgatctccca aaagttttat
     5581 atcaccaaat ctcatagttc cacctaatac cccaagatag gggataagat tgggtttatt
     5641 gagcaaatca attgtaattt cctgttccgg atgctgaata cctttagcgc ggagtttatc
     5701 aatttctatt tctgtaaacc attcattcaa ttcttcttta agttcaattg actcattgcg
     5761 taatgaatct attttattct gcaatatttt tagggaattg gttttttgtg atactaactg
     5821 atttaaagaa tttgtctcct tgttgcctgt acatgcagac ataacgaaga aaaagaaaag
     5881 tactatagta gtttgtggca gcatttaata attgtgtaaa actccctgat ggatgcactt
     5941 cgtgaatctc agtcacatag acttttttca aacccggtct tgaaaaggct gaaacaagct
     6001 tcaggacctg aaacccatac taccagaatc aggcagtgac agacgaattt ccaggacggt
     6061 ttgatgctga aattactaaa aaaaagaaaa aaaagcaact gattcagatc tgttttttaa
     6121 acgcatttgc gggcttaacg aggaaataac agctataaat gaatctccgt tttattttaa
     6181 cctatccctg tatataacag tcagtattaa agccagtacc cctactgcca cctgacaaat
     6241 cattccaacc gtattggctg ggatgaatgc cgcggatggc agttcctgca tgataaagat
     6301 attgcccccg ttatcttctt tcatccccac aacctgctgg gaaaaattca atgccacatg
     6361 gataccggtg ggcagggcaa tgccttttga ccaggcagca ccgagaccaa ataccagggc
     6421 ccagataccc ggacctaaaa tggccacggt ggtgtcccag cccatgatca tgtgatatac
     6481 ggcgaaagca actgcaacaa tcagctgggt gatcagaaac gaaaactttt tcttgagcaa
     6541 aatgaaggga taggaccgga aggccagttc ctcaaagagg gccagtggaa taatcgacaa
     6601 ataccagaag gctgcggaag tatcccaact attggaaaaa cggatacgga taccgccggc
     6661 tgccacaagc accaacatga tgcatgagaa gatgattgcc ccgtataaga atcctttgaa
     6721 aaaccggggc aaggtacccg attgccaaaa cagctcataa ttacggaatg ttcccttttc
     6781 agctttgata aaaccccaga ggagtaaaaa agtgccgatc gttccgccaa tcccatatac
     6841 aaactgttcc caactggccg ggaatgccgg tttgataaac cgacccacta taaaaagcag
     6901 gaaaataaac aggatccaga atccaggcac tttcaataaa ccggatgatc ggaaatcatg
     6961 catgagcagt tggttttgcg gatgggaaag gagacttgtc cggacccatc tatactgcta
     7021 acttactatt ctttctaata ataaagagat tcctctattc agcacagtga atgacaggta
     7081 ttatcagaac tgtcctatct tgggaaagaa aaacagcctc acaagggcat cacagtatgc
     7141 ggcaaacaga ttcagatatc atcattattg gcgcgggcgg tattgttcag catgcccacc
     7201 tgccagcgta taaaaaagca ggatatacgg tccggggtat ctatgataag gatattgcca
     7261 aagcaaaaat tgtggctgcc gacttcggat taccggtagc tttcgcctca ttggaacagg
     7321 ccattgatgc cgcgggccgc tcaacggttt ttgacctggc cattccggca gctgctttat
     7381 tatcagtatt ggaaaaaata ccacccggct cctccgtatt actgcagaaa ccgatgggtg
     7441 aaaacctgga acaggccaaa aatatactga aagtctgcaa tgagaaaaaa ctatcagccg
     7501 gtatcaactt ccagttacgt tatgcgccat ttatcctgaa agcaaaagaa ctgctgaata
     7561 gcggacaaat cggaacgctt tgtgatattg aaataaatat caacgtactc actccctggc
     7621 agctctggga tttcctgttt actttgccac ggatggagat actctatcac agtatacatt
     7681 atatggacct ggtacgacaa ctggccggag aacctgaatc agtttttgca agaacgatca
     7741 aacacccgga tatgcctcaa ctcgcctcag tgaaatccat gatctatatg gattatggag
     7801 aaaacctgcg ggccagtatt ctcaccaacc actgtcataa attcggtccc cgtcatcaac
     7861 attcctatat acggatcgaa ggcacccggg gagcaatcta tatccggctg gggctgctca
     7921 tggattatcc gcaaggcgtg ccggacgagt ttgaatacat tacgctcgat gaaaacgggg
     7981 agggaaagtg gaaatccatt tctattgaag gttcctggtt cccggatgct tttgcaggaa
     8041 gtatgggtga aatgatgaaa gcacagtcgg ataaaaaata taaacccgac aataacctgg
     8101 aagacgggct gaagaccatg cggtgggtgg aacgggccta tcaatctaca gaaaatggta
     8161 aatcagttaa aaaataatgc acccttttta caggatgcat tactgtttca gtaactactt
     8221 ttcttcaaaa acttttgctt aaaaaagctc tttcccgaat tccggactgc tgccggaact
     8281 ggctaagtta tccaacagct ttctggcaat cctctcggcg gcctcagccg gatcgttgaa
     8341 ttcaaattca taactgaggt tatagtcgtt atccatataa gtaacattgg tcttcatggt
     8401 caccgatcct ccggaaacga cattaagttt ccctttgaat tcagcggact ttacatccac
     8461 catgttttcc agaccattgt ccaccaccca gcgggccgct gccttggtac cggtggtaaa
     8521 cgtttcaaaa ccattgatta cggacagcga cgaattaact aagtccttat aggcgttttg
     8581 tttggtccag ttaatcgctt cacttaattt ttccagcgtt ttcacatacc ctttaagaat
     8641 tccgtttgct gtttcatatg cagcccacaa tgccgctttt tccggccctc tccacggctc
     8701 attattcaga tttatatcct ccgcttcatt tttcagccta cgtactttct tccgttgctt
     8761 ttcaactctt tccttctcat tttcaaatgc ctcaacatct gcttttgttc tggcaaggta
     8821 atcgcggctt tctttcagat catcctttgc ctttttgtat ctatcctgat ccgtctttgt
     8881 ttccttatta ataaaactga gtatttccgt attcaatatt tcaaacaaat cgcttttcat
     8941 ggccgccctg atttccatac ccggagaatg gtcaatactt ccactaccgc tgccatccag
     9001 atcagcactg aacttattaa acagtttgcc ggtagtagta aatttaaaac cggttccaga
     9061 tattttcaca tttacaccgg cagtcatacc cagtaaactt gcagtagcat caaaaaacat
     9121 aaacgggttt cctcctgcca gtgtggtttt tgtcagatcc accatgatcc ggctggagtc
     9181 tttgccattg gccccggtta acttgaagat ctccgtactt ccgtttttca caattacagg
     9241 tgcggccact gcttccccga aaaggccgtt ggaattagaa ccacccatat tcagtctggc
     9301 cctgaagcct gatatatatc ccgtaccaga aagctggaat ccctggtcca gggtctttcc
     9361 ggcaatcgtt gtggttttgg gcaccaggga aagtacggca ttggtgatct tgaaatcgcg
     9421 ggcaccaaca ggcaatttgt tgatctccgc tttcacggca tccgacatca gttcattaac
     9481 cacttgctga aaactgatct cattaactga taatgagacc gcgctttcca gcggatttgc
     9541 cgtattcagg gagcctgacc cggttccgga gaatgagccg attttgatcg taccggcaat
     9601 gccgataacc ggccttggca ccggggataa cacatcggca ccaatatcga gtccaagttc
     9661 cccgatccgg agtcctttta tgccaaaagg ttcattccag aaactgccct tattcatata
     9721 cgtgatcaaa ttgaaagtgg cagggggtga tacctcaccc ataccagtaa aatccaatga
     9781 tttctgatta tccggtttat aggaaaaacg cagcccggca ccgaatttca tatcggcagg
     9841 attcaactgc aggaatgact ccaacagatt aaacctttta ccaaactcca tattcttaaa
     9901 agtagtggcc gctttaacag tgggtaatgc caggggtaca gggaataagc cggataaagt
     9961 cagtgtcgtt ttggctctaa agccgggcag gtcggaggga accctgaatt cgcccattac
    10021 ctgtacacca gcgggtatat tggatccatc cagatttaat ttatgtaaca ctccggtcat
    10081 tttctttacc acaatttctt tatttgccac aataatagcc atatccttaa acccgatctg
    10141 atccatcggt gctaatattt cactgacttt agcaaaactg aatgaattgt ccggtgacaa
    10201 agccagcacc atttgtcctt ttggctgatc aaaaaaagct tccatacttc ccatattcca
    10261 tgctccggcg aggttgatct ggttatcgct gttaaaggaa agaaatgctt tattcaatgt
    10321 aaaattttca aaaggggccg gcagtacagg cacactgacc gaaacagccg acgccatcga
    10381 tgacaacata tcctttaatg acaaggaacc aatatttgcg gtaaatgacc atttagcatc
    10441 cgatgcggcc gtaccggaga gtgacaggtt aagcaggcca aacttcagcg cggaggaaaa
    10501 gtttgcctgg gtaatggtat ttgtccctgt tttatctaaa atcagtgtaa atgacggttg
    10561 actcagttgc atgccctgaa aaatgatcgg ggtggctgcc gcaggtggcc cgagttccac
    10621 catcatcttt gcctcactgg ctccggcatc ctgataactt aaactgagtt taagcagatc
    10681 catctttcca tttagccagg agggcaacca actactcagt tttttcccat caacggtcag
    10741 ctgactagta accccattgc tgaaacctgc ggtaaaggtg gctgaattac tggtagctga
    10801 gatatccgct ttaacattgc tcagcgaaaa aacggatagt gtaccggtga gcctgagccc
    10861 tttttcatct tctgagagaa tggtaaaatc cgcatcattc agcgataccg caccagtaaa
    10921 ggaaacgagc ctggagcgga tattgctcag cactgtttct ttgctgcccc cctgtgccct
    10981 gctgtactgt gatacgatgc acaaaaaaaa gagtgagaag aataagttct ttttcatatt
    11041 gaagatatta atacctcaaa tatcttcaat cattattctt ttaaaatacc ctgttagggt
    11101 gagaaatcag ccgcgaagat tacattttat tatgcatcgc gatacgaatt gcctctgcct
    11161 tgctgttgac gtgtaatttt ttataaatac ttttcacctg tgtacgaact gtttccaggc
    11221 tggtatccag gcgggccgca atcattttat agcttaagcc ttccaccata ttctgcaata
    11281 cctgtttctc acgcgctgtc agttcatcca tcttcacacc ataggtacgg ttgggcttaa
    11341 aatgttccaa cactttacgg gcaacggctt tactcatcgg tacttcacct actgtaatat
    11401 ccagtatact ttcccggatg cggggaaggg gcgtgctttt cagaaggtac cctgtggccc
    11461 cggcacaaag cgctttgaaa atatgctctt catcctggaa cacggtcagc ataatgatct
    11521 ccgcatccgg aaattttttc tggaaaagcc ggattccttc aataccattc attcctggca
    11581 ggccaatatc ggaaagaatc acatccggtt ttatctcggc cggatccaaa gccaggaacg
    11641 cttccactga attactggaa gctgttacgg caaattcttc ctgggcatcc aggtaagccg
    11701 taatactctt acgtatcgct tccacatctt caatgattgc aacctctatc atactacaaa
    11761 tttaacgggg aataacccgg gccacctacc cttttagggt gaaaatcata atcaaatcca
    11821 ctctctgcga acggtcagcg taaaccggtt ctcttccttc tttatttcaa gccgggcccg
    11881 gatacccaag gccctttcct gcatgttttt caacccatta ccacccagta ccccatacga
    11941 ttgcaactgg tttatatcat tcgtaatttc catttcaaac ccttctttcc cgttttccaa
    12001 tcggatttgc accccggagg gctggctgtg cttcactata ttatggatgg cttccttgta
    12061 aatcaggaaa agttcctgcc ggaccgtatt atccatctcc ttttgttcat caataccagt
    12121 caccgtaaag tccacttccg tattcaacgg ctgtaacatt aaatcggcgt ggtcacgcat
    12181 ccggtccagg aggctggatg ttttatcatg acccgctttt atactccaca caatatcgct
    12241 catagcatga atggcccggc ggctggtgtt gctgatattc ttcagttcgt ttttcttttc
    12301 ctcttcatca taaatattac tgctcaccat ttcactctgc agggatatct gggtaagaac
    12361 gctgccgagt tcatcgtgca ggtcggcgga tattttattc cggatcttct cgagtttcag
    12421 cacctggttg atccggtagc ggtaaaacgt ataaaccagt aaaccaagga ctgcagccag
    12481 taagacataa aaccaccagg tcttgtacca ggctaccagt acctttattt tcaaccggta
    12541 ctcattcctg gcccaggtat ccccggcaga agctcctttc accacaagtt cataatcccc
    12601 tgcgggaaga aaactataac tgatcatatt ggtattgggt gctgattgcc attctttatc
    12661 ccagccctgc agtttgtata aaaaccggtt ttttgatgta ttgcggtaag cgggtaacat
    12721 caccctgaaa ttaaataagc ggtcattatg actaaagatg atcctgttct ccgatacttc
    12781 cgatgcctgg aaaatgaata cagaatccct tttaccatca tacttactgt aactgactaa
    12841 ttgcggttgc aggttactca gactggtatc cacatcccgg gggtaaacag ccgtcacccc
    12901 atttagcccg ccgaaataaa tacggccgtc cgaggctttc agactggcca tccggttaaa
    12961 ttcattgtgc ggcagttcat cattcacatc ataattgata aatatttcct gggccggatt
    13021 gaaacggctg agtccgttat cagtactcag ccacaaaaaa ccgaattcat ccggtataat
    13081 actcatgata ttattattgg gcaatccatc gttggtggta tacacacggc tgtttttttt
    13141 caatgtatcc agccgtatca taccatgccg ggtacccagc caaaacaggt gtgcatcctc
    13201 ccggtacagg taatttatat ccagtacggg caataacagg gagggcacat tccccatgga
    13261 gaatgaacgc aacaagacac cctcgtcatt caacaggtac aatccatcat tgcctaccgc
    13321 ccagcatttc ccattaccgg catcaaaaaa attattcacc ccgtcgaacg ggtctccctc
    13381 cttattctta tacaataatc tgaaacgggc tttctgtcca ggccgtttcc ggtatattcc
    13441 gatggcagta ccagatgtta caaaaacggt cgaatcattc cggtaaataa acgcttttat
    13501 aggtgccagc acttcaaaag gacaggtatc tttactcacc ctgcccgtcc ttttttcaat
    13561 atgcaacata taattggctt ccgaactggc cagcaggtac tcctcttcat cgtgcatccg
    13621 gtaaacaggg gaacctccta catcataagc ccctccatcg ggttggtaaa acaaaaatgg
    13681 tttcgctgta tttttattac tggcccttat cacagacaaa acggtattat ttactgtact
    13741 gtttgcagca tatggataag aaccgatcca gatatccccg gacttatctt cggttattcc
    13801 ccggatactg atgcccgctt tctgcgggga aggcgtgctg taaagaaaat tgacaaaagg
    13861 actgcgcctg taatgaattt taaaaatacc cttccggctg caggtccata taataccgtc
    13921 agatccctgg taaacattca gccatgggta atcataaaaa aaatcattgg aataactgag
    13981 ggtgaattcc agttccttct ttgctaccgg gtcagtcaga tgaacagtat tccatttgat
    14041 attccattcg tttccagcct ggtcaagaat tcctgaagga cttgcatagg gacgtaacaa
    14101 tacatgttgg gcggcattca caaccagtgc cggatgctga atgactttat caaatcgcgg
    14161 gcccaacgca aacttgtatt caataccata aggatccatc agtactttta cgggccagtt
    14221 tttcccggcg tctttaacct gtcggaaatg ctcaaggaac ctgccctctg cactaatatg
    14281 gtcgatcccg ttaaaaaaag taggcaacca gaaactatga tctgcatcct ccagaattac
    14341 cggatgatcc aatccatcct ctctgacccg gatcgaaaaa tgataaataa accggagttt
    14401 gaaatggctg tcataacaga gtagcgtgtt gtcggccaag tagatcagaa tacggttctg
    14461 gctatcgagc ataattcttt ttacacgttg ctcagtaccg gtatatcctg ttaagggaat
    14521 cctgctggtc tttccggtaa ccggatccat tttcgaaagt ccatcattat acccaatcca
    14581 gatattacct tccttgtctt tcagcagact gccgatattc agtcctttca actgatccga
    14641 gccggaaaca tgctttgtat aatcataaaa agaatagcca tcatagcgga aaagaccatt
    14701 ggccgtaccc acccacataa aaccgcggtt gtcctgggtg atgcaattga cgagtttatg
    14761 cggcaatccc tgttccgtgg aaataaaatc aacggtaagt ccgggcgcct gtgcgactgc
    14821 cagattactg gtaaaataca accacaagaa caatatcctt cctttcaaag caatgagttt
    14881 ggtgtcatgg acaacaaaag aatctggctg gatattcctg ttgaactata ttgaaacaat
    14941 cctttcttgt ccgtgataaa tataagtaaa accaacagac atccggcatg gtacgctgac
    15001 cagatacggg caaaaaaagg gaagaggcgc cgatggctaa cctcttccct ttttcccttc
    15061 tcgcttctag cttcttgctt ctagcctctc tacacctcac cccaacccct ctcctttagg
    15121 agaggggcta aggacatccc gtaaacccct gctcaaaaaa ccgatcaatg ttcagggata
    15181 caggaaactg ttcttccctt ctcgcttcta gcttcttgct tctagcttcc ctctaaaatc
    15241 ttcttcttcc attcccgcca ctgtttccgc caccattccc tccactgttt tcactgcggc
    15301 gttcaacctg acgttgtgca ggctgagatt gcggagcggt ccggcgttcc atccgttgct
    15361 gtccgttact tccacttgat acaggacgac gctctattga ccggttatta ttcccctgaa
    15421 cggccccacc tccattatca ttacgtcttt caaaacgacg gggttctgta ttccctgaat
    15481 tgccattatc gggtctgcgt tgcggacgat cagcaccagg accggtgcga ggattattgc
    15541 ttccacccat acgtggattg tttccaccat tgttgctatt attatcattc cgttcaaaac
    15601 gacggccacc ggtattgttg ttgggtgtac caccgctttg aggttgtctg tccccgttgc
    15661 cattggtacg gaagtcgcga tttaccggac gaccaccacg tgtatcattg atacgaccat
    15721 tatcccggtt accattttca tttcttccaa aaccattgtt gcggtttccg ttttcaggac
    15781 ggccattagc acctccatta ttaatacggc cattgccatt gttgcggggg gcagttctga
    15841 aatctctgtt gcggtcgtag cgctcaaaac gctgtggcgc ataaccacgg ccttcatcac
    15901 gacgcacatt gggacggtaa attcttactt cattattcct gaaataagta cggccgggtg
    15961 aataactgtc ccggaaacga accggagtga tacggccgca atagcgttct gcttcccaac
    16021 ggctcggccc tgaacgataa ccccaacgac caccgatatt aaagttgatc acggtagtga
    16081 cactgaaaat ggaagaattg cggcgatagt ccacacaata atctctcacc cttgggtaaa
    16141 gaatataccg tcgtggcgta aaggaccaga aatcataggg cggtgcatag ctgcccatat
    16201 tgtaattgac attgatgctg atacccggac gtatcggtgc ccagccataa taatcacctc
    16261 catcgcgcca ggctacccag gccggagacc attcatatcc cggtacccaa taccatccgt
    16321 aatgctgatc ctggtcccag cgaccatagt ggaacggagc ccagccccag tcataatcgg
    16381 atacccacat ccattcggca tcctccgtcc agacccaatg tccttcagta ctgtaaggcc
    16441 ggaaatccgg gcccgcatcg ggaatccaga tataaccatg gtccgggtga tccacccact
    16501 gaccataagg actcagctga tcatagaagt cctggtaaga aacctcccgg ttccggtcat
    16561 tgggactgta ctgatcatcc tgggtgtagg aatcattctg attgtactgg gccaccgctg
    16621 actggagcca ggaactgccc agcactatga acagtaccag agcaggcaat gtaaatgttc
    16681 gtttcatgtc ttgaagtttt agcttgttgg tgaaatcacc aacaagatga gtgatgatgt
    16741 cctgttggtg ttttcaccga caggttcttg ttggcgttct caccaacaat atctactagc
    16801 acagacgcga ttactgtgcc accggataac aattgttagc cggaaggggt cttttaagaa
    16861 aacatttggt gaaaaatttt cacaattggc ggcgtcaccc ttcgatacgt cccgcatgac
    16921 tttattcaac ttccctgtat gttaacttta gcgggactac tccccgcatt aggcgggaca
    16981 ggcaggatga cacgatcatt tcgcaccgtg atgggaaaag tagaaggact ccagccccga
    17041 aggggtgaca caattatagc ccggcaataa cgaagatgcc cgcaaagccc cgaaggggtg
    17101 acacaattgt aatttgtaac attaaacatc tttcactcct taacgccgtc tatagagcaa
    17161 tcagaaccat cctaccaaaa caaatgcaac gcaaacgagg ctacaagcct cgttgagcgg
    17221 gcacatgtac attttctcag gtcgctaaaa gcgacaaaat aatgccccgc agctggaaac
    17281 tacggggcca gtcaagaaca ttacttcatt gcctgtcttc ttcaatcatt attatttctt
    17341 agaaggggct gtttcccgac acctgacacc taacaccttt accaatactc cggaaaaaaa
    17401 ggtgtagatt tagtaaacaa gaatcaacaa ctacaacaac catggaacaa cgcttcatca
    17461 acctctacga cgagtacacc cacaagccac ttccccggca ggaatttatc aaacgattga
    17521 cgatgatggc cgggagtact gcagccgctt tagcccttct gcccttgctg gaagtaaatg
    17581 gtgcccattc ggcgataacc ccacaagatg atttgtttac cgaacgaatc agttatgcag
    17641 gcgtgaacgg agatatgcag gcctatgtgg cccggccgaa agatgataaa aaatacccgg
    17701 ccgtggtggt gattcacgaa aaccgcggac tgaatgcaca tattgaagat gtagcccgcc
    17761 gggcagccaa ggccggcttc ctcgctattg ccccgaatgc cctgtcctcc ctgggcggca
    17821 caccggaaaa tgcagatgaa gcaaggacaa aattccagca actgaagccg gaagacaacc
    17881 tgcagaattt tattaaagtc tttgattacc tcggcacccg taaagatgcc aatggaagtt
    17941 acggttgtgt gggattctgc tggggtggtg ccatgagcaa taacctggca gtaaatgtgc
    18001 cttcattaaa agcagcagtg gctttttacg gcagacaacc tgctaccgaa gatgtggcaa
    18061 aaataaaagc ggccgtacag ctgcactatg gctcaatgga tgagggcgtc aacaaaggca
    18121 tagctgccta tgaagaagcc ctgaagacga ataagatcgc ttacgaatta tacatgtacg
    18181 aaggcgccca gcatgctttc cacaacgata ccgctcccac ccgttacaat gaagccgccg
    18241 ctaagctcgc ctgggaacgc acgatgtcct tttttcagaa acagcttaag tagggattat
    18301 taattattgg attattgatt attataaagc agaaagagta ttatagaaat agccggcact
    18361 catagtaccg gctatttata taatacaccc ctactcctta cttataactt aatacttata
    18421 acttaatact tataacttac aacttccctt tacctcctca ccctgaaatt acacactacc
    18481 gtccttggag ttcctccaag attcatttgc accgtaaagt tgccttccac aaagccgcca
    18541 ggggctgcac caaataccgt tgtattaacg gaagctgcgc ctgctgcaaa actctgcgag
    18601 ttggtcatcc ctggtccggc actcacatat acaaaatcaa gaggaagacc agtcgctact
    18661 gattgattat gacggaacga aaactgaata cccccgcttg ttgttcctga attctgacgg
    18721 gcacctgaaa tccaggtatt aaacagataa ggagcagcac cggttgagtc attataatta
    18781 atgtaatctg ccggggtagc aagggtaaac ggcgttccat caatcattac ttctatatac
    18841 tccagcgaag atgtgccaca ggcctgcagg gtacccacgt tcaccgtacc ggttgtacca
    18901 gtaccggaaa ccgggatcga ttgttgcagg gtggcataat caactcccag aactgtataa
    18961 ttgatggccg tattcgtgca acggggaata gtaaccgaaa ataccccgtt ggtcacggct
    19021 acggtgtact ggtgtccgcc ttcaatatag atcgctgcgg caccattgac cacattggta
    19081 gtggcacagg tggtaagggt gccggaaata gtgaggctgg tggctgccgg aagtgtaacg
    19141 gtgataaccg gcaatgtact atttgttgta aacggaccga ttgcctgtgt atagaacgga
    19201 ttgccgcaga tatccagcag ttgtaattcg aggttctcat tcttcggcac tttaccacaa
    19261 agactaccgg ttgaatccgt acgtccatag ccatagctgc cgtttacgat ccgcttgatg
    19321 cgtacctgta cattgttcag cggggcccca tgatcatcct tgaatgacat acaaagatca
    19381 atcagcggaa atggtgcatc gcagttccag aaagagaaat gactgacttt ggctacatac
    19441 atggctccgg tcttaccggc ctttccctcc tgtttccagc gggcggtggc ttcatcaaaa
    19501 tgccagagat cgatggaatc aggagcagca gcctgtaagg aggccgggat cgggaaactc
    19561 agctcagcag tcttgcccgg agctacttta agcgcctgac cggtaccgcc ggtcatttcc
    19621 acgccaatca taccaaaagt agataaccct ctttcggcac cattggtcag taccccccgg
    19681 aggtcaccca tcatatt
//
LOCUS       DAOPMW010000023        19140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2765_length_19140_cov_10.731150, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000023 DAOPMW010000000
VERSION     DAOPMW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2765_length_19140_cov_10.731150"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLU11_02335"
     CDS             complement(<1..1150)
                     /locus_tag="PLU11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPN57974.1"
                     /translation="MKYLPLNPEIFVQNRQRFVRQMQKNSIAIFVSNDEVPSNGDAIY
                     SFKQNSDLFWLSGVTQEDSMVILFPDNPDPKYREVLVLVRPNELKEKWDGKRLRVKEA
                     QDMSGIKTIVWLDSIDGLLQPWIHLADNIYLDSNENDRKASLVRSRDYRFIDEMKSRY
                     PLHNYLRAAKIMKELRAIKTALEVEVMQKAMDITEHTFRRLLKFVKPGVMEYEIEAEI
                     FHSFLSQRATGPAYGSIIASGDRARTLHYVSNSEECKDGELILMDFGAEYGGYCADLT
                     RTIPVNGKFTRRQKTVYNACLNIHNYCRSILKPGITIVDYTDKIGEEATQQFLKIGLI
                     KKSDVKNEDPENRAYRKYLYHGISHHLGVDVHDLGTKTEPVKAGMVFTI"
     gene            1445..3049
                     /gene="pckA"
                     /locus_tag="PLU11_02340"
     CDS             1445..3049
                     /gene="pckA"
                     /locus_tag="PLU11_02340"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPN57975.1"
                     /translation="MSVPTIAIPAQHLHKIGLKNNGSIHYQLSPEELIQDTLRIGDGV
                     LNDTGALVIQTGEFTGRSPKDKFTVKDESTADSVHWNDFNIPIEPGYYDIIFDKVTDY
                     MNRLPELWVRDCYACADPRYRLNVRVVNEKPWNNLFAYNMFLRPAEEELESFTPDWHV
                     ITVPGLKLDPAVCGTRQHNAAVVSFKHKMILIAGTGYTGETKKGIFTILNYILPHEKD
                     VLSMHCSANMGEDGDTAIFFGLSGTGKTTLSADPNRKLIGDDEHAWTNDNIFNFEGGC
                     YAKTINLTEEKEPEIFNAIRPGALVENTVFFEGTNRINFDDGSITENTRVSYPLHFIS
                     NAQEPSVGGLPKNIFFLTCDAFGVLPPVSKLTPGQAMYQFISGYTAKVAGTEAGVTEP
                     KPTFSACFGAPFLPLHPGKYAAMLGKKMQENNVNVWMINTGWTGGAYGTGSRMKLKYT
                     RAMITAALEGTLNNVEYMNDPIFGMAVPTSCPDVPAELLQPRNTWADKNAYDEKAKYL
                     AGLFVKNFEKYKDGVSEEILAAAPRA"
     gene            3307..3843
                     /locus_tag="PLU11_02345"
     CDS             3307..3843
                     /locus_tag="PLU11_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HPN57976.1"
                     /translation="MKRIFSLLLFSLSVSATLAQEAGEDILTWSASRKLTWSDYKASP
                     NPKSDAAATTSTSLSIDYHISGSQFSYTIKSWFSRSRSWVRNQTDHILSHEQGHFDIA
                     EAYARKLHKRMSAYRFNKNNYQKELNRIYNEVADEKAAVQQQYDRETRHSIDEVKQAE
                     WLKKISRLLDELKDFADY"
     gene            complement(3956..4591)
                     /locus_tag="PLU11_02350"
     CDS             complement(3956..4591)
                     /locus_tag="PLU11_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPN57977.1"
                     /translation="MAKAKKPKHIAIAGNIGAGKTTLTEMLSKHYKWIPNFEDVDHNP
                     YLMDFYEDMPRWSFNLQIFFLNSRLRQLVEIQKGTETVIQDRTIYEDANIFAPNLHEM
                     GLMSKRDFDNYFNFFQTLKTLVQPPDLLIYLNASVPTLVGQIQKRGREYEENIRLDYL
                     KKLNEYYNKWIGDYKEGKLLVIDVDKNKFAERDEDFGEIIRKVDAELYGLF"
     gene            complement(4686..5690)
                     /gene="trpS"
                     /locus_tag="PLU11_02355"
     CDS             complement(4686..5690)
                     /gene="trpS"
                     /locus_tag="PLU11_02355"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPN57978.1"
                     /translation="MAAKKELVMSGIRPTGFLHLGNYFGAVRNYVRMQEEFDCYFMVA
                     DLHSLTTHPDTKELKTNVHRVLSENIACGLDPEKVALYCQSHVYETSELYLYLNMLAY
                     VGELEKTTTFKDKIRLNPENINAGLLTYPVLQAADIILHRAKYVPVGKDQEQHLEMAR
                     NYVNRFNHRYGEVFPEPVAFNYGEELVKVPSLDGKGKMSKSENQLATLYLSDDDEMIR
                     KKCKKAKTDAGPTEPNSPMPDYIENIFLLMKLVSEEDVIRKFKEDYNACTIRYGDMKE
                     QLGEDMVRFITPIREKADAIRNNEAYLKQVMEMGAEKARKSARVTMELVREAIGLNYF
                     "
     gene            5797..6087
                     /gene="gatC"
                     /locus_tag="PLU11_02360"
     CDS             5797..6087
                     /gene="gatC"
                     /locus_tag="PLU11_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPN57979.1"
                     /translation="MEVNDPLVEKLAHLSRLQFTDTEKAGIKKDLQRMIGFVEKLNEL
                     NLEGVEPLLHMSPEVNVLREDEIRGSVSREAALRNAPLHDEQFFKVPKVIKK"
     gene            6089..6802
                     /locus_tag="PLU11_02365"
     CDS             6089..6802
                     /locus_tag="PLU11_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN57980.1"
                     /translation="MAQGIIHLEDIQKSYYMGKQALPVLKGISMDIFKNEYVALMGPS
                     GSGKSTLMNILGCLDTPTAGRYILNNNDVSTMADNALAEVRNKEIGFVFQQFNLLPRL
                     TAAENVALPLVYAGMNKKLRNEKAMQVLEMVSLTDRSHHKPNELSGGQCQRVAIARAL
                     VNDPSIILADEPTGNLDTKTSYEIMEIFNTIHNNGNTIVLVTHEEDIANHAHRIIRLR
                     DGVVETDRKNEKAGQYSHH"
     gene            6873..7433
                     /locus_tag="PLU11_02370"
     CDS             6873..7433
                     /locus_tag="PLU11_02370"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPN57981.1"
                     /translation="MATRIYTKVGDKGSTSLIGGTKVPKSHLRIEAYGTVDELNSVIG
                     LCRDLIVEENSREILREVQDRLFTIGSSLACDPIKEPKMRLPDLKETDVELLEKEMDR
                     MNEELPEMKNFVLPGGHPTVSHLHIARCVARRAERCCVRLELESLEVEPVILKYLNRL
                     SDYLFILSRYTGMKLNAPEIPWKPRI"
     gene            7524..8387
                     /locus_tag="PLU11_02375"
     CDS             7524..8387
                     /locus_tag="PLU11_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN57982.1"
                     /translation="MRNTCFFLFLFIYLSSCQPDPVNPPNTGPLRDTTLFNVPYGSDP
                     AQKLDLGLPANRTNNSALVLVIHGGGWATGDKAELNWLLNGLKARGFAVANINYRLTL
                     NTPDNYKMQLDDVDSALNYTLRQSSIQTFNGQKLYLVGHSAGGHLSLSYAYTRNSTGR
                     IKAVGSLAGPTDLYSMSYYNFNIYNLILEPYLGLPLFPASAEATDRYKACSPFYQATA
                     TVPASIFFHGALDVVVTPDQSSSLNNKLGTLGVDHKLVSYPLTFHDWWTDGTKVTNTL
                     DELKSWFNTHP"
     gene            complement(8378..9034)
                     /locus_tag="PLU11_02380"
     CDS             complement(8378..9034)
                     /locus_tag="PLU11_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN57983.1"
                     /translation="MISGKNIYKRYGTVEVLRGVDVEISRGEVVSIVGPSGSGKSTLL
                     HILGTLDAADSGEVRMNQVLLQSLSGKKMAAFRNKHIGFVFQFHHLLPEFSALENVCI
                     PGWLSGRGKAVVKQEAEKLLGMLGLANRMENKPNQLSGGEQQRVAVARALINKPDIIF
                     ADEPTGNLDSANAKDLHQLFFDLRSQFSQTFLIVTHNEELATLSDRVLHMRDGIIVQG
                     "
     gene            9250..9471
                     /locus_tag="PLU11_02385"
     CDS             9250..9471
                     /locus_tag="PLU11_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPN57984.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYSIRSGAPIEVVENLQELE
                     DEGDVYETIEDIWPDYPTQDDFLFNEDEY"
     gene            complement(9525..11228)
                     /locus_tag="PLU11_02390"
     CDS             complement(9525..11228)
                     /locus_tag="PLU11_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPN57985.1"
                     /translation="MANYYTDTTELKFHLTHPLMQKIVALKERNYTAHEQFDYAPVDF
                     EDAMDSYDKVLEIVGDICANTVAPNAESVDAEGPLLIDGHVKYAAGTQKNLEAIVKAG
                     LMGITLPRKYDGLNFPIVPYIMAADMVSRADASFVNIWGLQDCAETIHEFADEEQQAR
                     YLPRIAAGETAAMDLTEPDAGSDLQAVQLKATLVDGKWFLNGVKRFITNGDGNISLVL
                     ARSEEGTADGRGLSMFIYDRDSNAVTVRRIEHKLGIIGSPTCELVFKNAPAELVGQRK
                     FGLIKYVMALMNGARLGIAAQSVGIADAAWREAVSYAKERVQFGSSISKFPAVYEMIS
                     KMKAKVDAIRSLLYETARFVDVYKAYEHISKERSLTPEERTELKAYQKLADIFTPMSK
                     GLSSEVCNQVAYDALQIHGGSGFMKDYPIERIYRDARITSIYEGTTQLQVVAAVRGVT
                     TGGYLAQMKAYEQMEVKPELLGYRNYISEMTNEYEEAVKAVTEVGNNEYTDFHARRLV
                     EMAGNIIMSHLLVLDTNRDHQYWKSLDIFLKIARGENRGKMEFIKITNPADLGKYKID
                     G"
     gene            complement(11268..12287)
                     /locus_tag="PLU11_02395"
     CDS             complement(11268..12287)
                     /locus_tag="PLU11_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPN57986.1"
                     /translation="MNNVCVYCEFEEGRIQEVSYELLTKGRSLANQLNCKLEALVLGE
                     GLNGLEKEVSRYGVDILYMADDKRLYPYTTLPHTAVAVGLFKQTHPQIVLMGATSIGR
                     DLGPRISSSLTSGLTADCTNLEIGEYEDKKAGKKYENLLYQIRPAFGGNIVATIVNPD
                     NRPQMATVREGVMKKETISDTHKAELIYLDAGQYLRDEDLVVKIIERHMETSKVNLKN
                     SSIIIAGGYGVGSKENFNLLYELAHTLGAEVGASRAAVDAGFTDKERQIGQTGITVRP
                     KLYIACGISGQIQHIAGMQDSAMVISINNDANAPINKLADYVIQGNLETIIPKMIRYY
                     KKNIK"
     gene            complement(12317..13183)
                     /locus_tag="PLU11_02400"
     CDS             complement(12317..13183)
                     /locus_tag="PLU11_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPN57987.1"
                     /translation="MYKIIVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLNA
                     LELALEIKDQYPDTKITLLTMGPVRAAEILREGMYRGADAGVLLTDKAFAGSDTLATS
                     YALSCAIRKIGEADLILAGRQAIDGDTAQVGPQVADKLGIPQITYAEEIISLHDRTIQ
                     VKRRLERGVEVVECTLPVLITAHSTAPECRPRNAKRLMRFKRATTLSERHTTAEDYLA
                     VHNDQAVLITEWCAKDVNSDLVQLGLGGSPTKVRQVENIVFQAKESKVISTDDGEIES
                     LMQELISQHTIG"
     gene            complement(13481..14296)
                     /locus_tag="PLU11_02405"
     CDS             complement(13481..14296)
                     /locus_tag="PLU11_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN57988.1"
                     /translation="MSNQLLKGKKGIIFGALDENSIAWKTAVKCHEQGAQIVLTNAPV
                     ALRMGEINKLSELCGNAPVIGADVTLLEDLKHLLQESMKHFGGKIDFILHSVGMSINM
                     RKGHHYTEISYTHNHKTLDISALSLHKLLHTAWNMDAISEWGSVVALTYIAAQRVFPD
                     YNEMADAKALLESITRNFGYHYGVRNKVRINTISQSPTRTTAGSGVKGFDGFISYAEK
                     MSPLGNASGEDCANYTVTLFSDLTRFVTMQNLFHDGGFSFTGVSHAVIEKMEG"
     gene            complement(14375..14581)
                     /locus_tag="PLU11_02410"
     CDS             complement(14375..14581)
                     /locus_tag="PLU11_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57989.1"
                     /translation="MKLKSLMIMGLILLSLLPLFLLYKWLQVKMKPRHSLQHFFGFMA
                     VMFVIVFSYTFLLVFGIRLLFPGA"
     gene            14701..15777
                     /locus_tag="PLU11_02415"
     CDS             14701..15777
                     /locus_tag="PLU11_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPN57990.1"
                     /translation="MHIRTIITGSGCYIPPVIKTNRDFAIQTFYGEDTNRIETPTEEI
                     VTKFQDITGIEERRYAAEEMNSSDMATLAARQAIEDSGINPEELDQIIVAQNFGNVIK
                     HTIQTDVLPALASRVKHDLGIKNPNCVAYDILFGCPGWVQGVIQADTYFKAGAAKKAL
                     VIGTETLSRVIDVYDRDSMIFSDGAGATILEAQDVDEKGPGILSWSVQSHCLDEAYYL
                     YLGKSNFPGSDPRIRYIKMKGRKVYEYAMQHVPAAMKDCLDKAGVSISDVKKVFIHQA
                     NEKMDEGIIRRLFKLYNIKEIPEHIMPMSIHKLGNSSVATIPTLFDMVKRGLWDNHDL
                     KAGDIVLFASVGAGMNINAICYRM"
     gene            15843..18269
                     /gene="pheT"
                     /locus_tag="PLU11_02420"
     CDS             15843..18269
                     /gene="pheT"
                     /locus_tag="PLU11_02420"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPN57991.1"
                     /translation="MTISYKWLSEYLPVTVDPERLSRILTSIGLEVESMHPYEEVKGG
                     LKGLVIGEVLHTEKHPNADKLTLTKVNTGGTEPLQIVCGAPNVAAGQKVVVATVGTTI
                     YPSAGDPLTMKVAKIRSVESHGMICAEDEIGLGSSHAGILVLPADTKVGTLAAEYFKP
                     YEDIIYEIGLTPNRMDAMSHWGVARDVCAYLSHHDKKDLRPKIPHSNGLKADSLSFPI
                     EVKIENDTACPRYSGISIDKVTIAESPVWLKQKLKAIGLRPINNIVDITNYIQHETGQ
                     PLHAFDADLIRGRKVVIKNLPEGTPFVTLDEKERNLSADDLMICDEEGGMCIAGVFGG
                     LHSGVTEKTKTIFLESAFFEGIGLRKTSFRHNLRTDAASRFEKGTDISATVNVLKRAA
                     LLIKEICGGEIASEIVDIYPKKKEKADVQIKWHYIKKLSGKNYHPDSVKNILSSLGFE
                     IIKEGIDELRVAVPFHKPDISLPADIVEEIVRIDGLDNIDIPESITITPAVEDNYARE
                     VVKEKTADYLVGLGYHEMMTNSITNAAYFSAAELETMVTMKNSLSAELNILRSSLFET
                     ALEVVAHNLNHKNSSLRLFEFGKAYSTSGAGKYHEAEQLCILISGNSREEEWKQKAVA
                     ADFYALKGTVEAVMRGLGIKADSVEAMEVPKLENHIVYKLNNHVIAGAGQVSKGVLDQ
                     FGIKQPVFYAGLNWELISDLASNHNPKIREIPRYPQVQRDLAMIVSRELPWQEVENTV
                     HKIRLDKLRDIKLFDIFESEKLGTGKKSLAVNFTFLDEEKTLTDKEIDGWMNKIMGAL
                     EKELQAEIRK"
     gene            18266..18553
                     /locus_tag="PLU11_02425"
     CDS             18266..18553
                     /locus_tag="PLU11_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57992.1"
                     /translation="MNTTEEQLKRIQDKLQQLLKQQVAARRENEKLKEELGAAREKLA
                     TQQKSTDELKQQVTVLKMNAGGMNDMDKKELEKKINSYLKEIDRCIALLGE"
     gene            complement(18554..18811)
                     /locus_tag="PLU11_02430"
     CDS             complement(18554..18811)
                     /locus_tag="PLU11_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN57993.1"
                     /translation="MFAKVKSSVFIDALQLQLTAIKGYHPLRGVLKDDTGSVCRIIEQ
                     KIFRDMEELTWAGLNDLPYGRYTLELSHGDDEMKMNLVKRV"
     gene            18932..>19140
                     /locus_tag="PLU11_02435"
     CDS             18932..>19140
                     /locus_tag="PLU11_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPN57994.1"
                     /translation="MDDLILISALIGDRTYRIKIEPRDEEMVRRTLKTINEKIVEFKT
                     EFAGKDMQDYIAMVMIWLATEMQSAP"
BASE COUNT         5147 a         4583 c         4415 g         4995 t
ORIGIN      
        1 caatggtaaa gaccattccg gcttttaccg gttctgtctt ggtacccagg tcatgcacat
       61 ccaccccgag gtgatgtgat atgccatgat ataagtattt ccgataggcc ctgttctccg
      121 gatcttcgtt cttcacatcg gattttttta tcagcccgat ttttaaaaac tgttgcgtgg
      181 cttcctctcc gattttatcg gtataatcaa cgatcgtgat accgggtttt aatatactgc
      241 ggcaataatt atggatattg agacaggcat tatacaccgt tttctgacga cgggtgaatt
      301 tgccattgac cggaattgtc cttgtcagat ctgcacaata tccgccgtac tctgcgccga
      361 agtccatcag gatcagttca ccatccttgc actcttcact gttgcttaca tagtgtaagg
      421 tacgggcacg atcaccactg gcaattatac ttccataggc gggtccggtt gcgcgttggc
      481 ttaggaaaga gtgaaagatt tctgcttcta tttcatattc catcacaccg ggcttaacaa
      541 actttaataa gcggcggaat gtgtgctccg taatatccat cgccttctgc atcacttcca
      601 cttcaagtgc tgttttaatg gcacgaagct ctttcataat ctttgcggca cgcaggtaat
      661 tatgcaacgg gtaccggctt ttcatttcat caataaagcg atagtccctt gaacgtacga
      721 ggctggcctt gcggtcattc tcattactat ccaggtaaat attatcagcc aggtgaatcc
      781 agggttgtaa taaaccatca atactatcca gccatacgat ggttttgatg ccactcatat
      841 cctgcgcttc ttttacccgg aggcgtttgc catcccattt ttccttcagt tcgtttggac
      901 gaaccagtac cagcacctcc cggtatttcg gatcgggatt atccggaaac aggatcacca
      961 tactgtcttc ctgggttacc ccgctcagcc agaaaagatc gctgttctgt ttgaaggaat
     1021 aaatcgcatc cccgttactg ggcacttcat cattacttac aaaaatggca atggagtttt
     1081 tttgcatctg gcgcacaaag cgttgccggt tctgtacaaa aatctccggg ttcaatggca
     1141 ggtatttcat atcagttgat taaggaattt gaggaaaaat gaaaaatcct gctaaagata
     1201 gcatcctatc cttttatgcc cctgactttc atcacaaaac aatgcaggaa ccctcgattt
     1261 ggagaaaatt cagcagaaac tgccactgat tgaatattgg atgatataaa gccatgaaaa
     1321 tgaataaata attgtcccaa aaactgattt tttgacattc tctaatgtgt cgcacgaaca
     1381 cttgtttgta aaatattggg ttttggtatt acctttgtcg cttcaccaaa tgagtgcgcc
     1441 atatatgtca gtaccaacga ttgctattcc tgcccaacat ttacacaaaa tcggcctcaa
     1501 aaacaatgga tccattcatt atcagcttag tccggaagaa ctgatccagg acacccttcg
     1561 catcggcgat ggagtgctga atgatacggg ggccctggtc atccagacgg gagagtttac
     1621 cggacgttct cccaaagaca aattcacggt gaaagacgaa agcaccgcag acagtgtaca
     1681 ctggaatgat tttaatattc ccatcgagcc cggctattac gatatcatct tcgataaagt
     1741 caccgattat atgaaccggc tcccggaatt atgggttcgt gattgctatg cctgtgccga
     1801 tccccgctat cggttaaatg tacgggtggt caatgaaaag ccctggaaca acctgtttgc
     1861 ctataatatg tttcttcgtc cggccgaaga agagctggag agttttacac ctgactggca
     1921 tgtgatcacg gtacccggtt taaaactgga tcctgcagta tgtggtaccc gtcagcacaa
     1981 tgcggctgtg gtatctttca aacataaaat gatcctgatt gccggtacgg gttataccgg
     2041 tgaaaccaag aaaggcattt tcaccatcct caattatatt ttaccacacg aaaaagatgt
     2101 attgagcatg cattgcagtg ccaatatggg tgaagacggt gataccgcca ttttcttcgg
     2161 attaagtggt accggaaaaa caacgcttag tgcagacccc aaccgtaagt tgatcgggga
     2221 tgatgaacat gcctggacca atgacaatat tttcaatttc gaaggcggct gttatgccaa
     2281 gaccattaac ctcaccgaag aaaaagaacc ggaaatattc aatgcgatcc ggccaggtgc
     2341 ccttgtggaa aataccgttt tctttgaagg aacgaatcga atcaattttg atgacggatc
     2401 aattactgag aacacccggg tctcctatcc cctgcatttt atcagtaatg cccaggagcc
     2461 ttccgtggga ggtttgccaa aaaatatttt cttcctgacc tgtgatgcgt ttggggtatt
     2521 gcctcctgtc tcaaaactta ctcccggaca ggcgatgtac cagtttatat ccggatatac
     2581 agccaaagta gccggtactg aagcgggggt aacggaaccc aaacctactt tcagtgcctg
     2641 ttttggcgcc cccttcctgc cccttcatcc gggcaaatat gccgccatgc tgggtaaaaa
     2701 aatgcaggaa aacaacgtga atgtctggat gatcaatacc ggctggaccg gtggagcata
     2761 cggcaccggc agccggatga aactgaaata tacccgggcc atgatcacgg ccgcactgga
     2821 aggcacattg aacaatgttg aatatatgaa tgatcccatt ttcggtatgg cggttcccac
     2881 cagttgtccg gatgtacctg ctgaactcct gcagccccgg aatacctggg ccgataaaaa
     2941 tgcctatgat gaaaaagcaa aatacctcgc cggattgttt gtgaagaatt ttgaaaagta
     3001 taaagacggg gtgagtgagg agatacttgc cgcagcacca agggcataag tacgaagtac
     3061 gaggtacgaa gtacgaaagg cattccgtaa aacgaggaag agccgtttac taagattcaa
     3121 cctcctaaga aggggctatt gtccgaagca tcggactact aactaccgac ttctaactac
     3181 agactaattc tctggatggt tgtttgaaat attcccggag ccgatggttc cgggattttt
     3241 atttttttta acgccgggcc ctgcatccaa taaaattttt ctcccctctt ttcgttccat
     3301 tacaacatga aaagaatttt cagtttactg ctttttagct tgtccgtttc cgccacgctt
     3361 gcgcaggaag ccggcgaaga tatccttacc tggagtgcaa gccgcaagct gacctggagt
     3421 gattacaaag ccagtcccaa ccctaagagt gatgcggcag ccaccaccag cacttccctg
     3481 agtatcgact atcatatatc gggcagccag ttcagttata cgatcaaatc ctggttttca
     3541 agaagccgtt catgggtacg taatcaaaca gatcatatcc tgagtcatga gcaggggcat
     3601 tttgatattg ccgaggccta tgcccgcaaa ctgcacaaac gaatgagcgc ctaccggttc
     3661 aataaaaaca attaccagaa agaactcaac aggatctaca atgaagtggc cgatgaaaaa
     3721 gcagcggtgc aacaacaata cgaccgggaa acccgccaca gcattgatga agtcaaacaa
     3781 gccgagtggc tgaaaaagat cagccggtta ttggatgaac tgaaggactt tgctgattac
     3841 taattccggt tgatatagca accccatcgg tagaattaag gggaatgccc aaaaagaaaa
     3901 aacctgcccg gtctgaatac cgcaagcagg tcttaatcaa ctattttatt ggctgttaaa
     3961 acagaccgta gagttcggca tccactttcc ggataatctc tccgaaatcc tcatcccgtt
     4021 cggcaaattt atttttatcg acatcaatga ccaggagttt tccttcctta taatcgccga
     4081 tccatttgtt ataatattca ttgagttttt tcaggtaatc cagccggata ttctcttcat
     4141 attcccggcc ccttttctgg atttgtccca ccagggtggg tactgaagca ttcaggtaaa
     4201 tcagcagatc cggcggttgt accagggtct tgagggtctg gaaaaaatta aagtaattat
     4261 caaaatccct tttgctcatc aggcccattt catgcaggtt gggcgcaaag atgttagcat
     4321 cctcatagat ggtacggtcc tggatcactg tttccgttcc tttctgaatt tccactaact
     4381 gacgcagccg gctgttgagg aagaatatct gaagattaaa actccatcga ggcatatcct
     4441 cataaaaatc catgaggtag ggattatgat ccacatcctc aaaattggga atccacttat
     4501 agtgcttgct gagcatttcg gtcagggtcg tctttccagc gccgatattg cctgcaatag
     4561 cgatgtgttt tggtttctta gcttttgcca taactgctta aattccggat acaataatat
     4621 tcccgaaagg gagggcttca aagtaagccc tttttcggac tttgagattc ggtaatccct
     4681 gttttttaaa aataattcaa cccgatagct tcccgaacca gctccatcgt gacccgggca
     4741 cttttacggg ctttctccgc ccccatttcc atcacctgtt tcaggtatgc ttcattattc
     4801 cggatggcat ctgctttttc ccggatgggg gtaataaagc ggaccatatc ttctcccagt
     4861 tgctctttca tatcgccata gcgtatggtg catgcgttat aatcctcctt gaatttccgg
     4921 attacatcct cttcactgac cagtttcatg agcaggaaaa tattttcaat ataatcgggc
     4981 atggggctgt tgggttcagt tggtcctgca tccgttttag cctttttaca tttttttcgg
     5041 atcatttcat catcatctga cagatataat gtagccaatt ggttttcact cttactcatt
     5101 ttacccttac catccaggct gggcactttt accagttctt ccccataatt aaaagccact
     5161 ggttccggaa atacttcacc atagcggtga ttaaaccggt tgacataatt acgggccatt
     5221 tccaggtgtt gttcctgatc ttttccaacc ggaacatact tggcgcgatg caaaataata
     5281 tccgccgcct gcaaaacagg atatgtcagt aaaccggcat ttatattctc cgggttgagc
     5341 cggatcttgt ctttgaaggt ggtggtcttt tccagttccc ccacataagc cagcatattc
     5401 aggtagaggt agagttctga agtctcatat acatgactct ggcagtaaag ggccactttt
     5461 tcgggatcca gtccgcaggc aatattctcc gacaatacgc ggtgcacatt tgttttcagt
     5521 tcttttgtat ccggatgggt ggtcagggaa tgcaggtcgg ccaccataaa ataacaatca
     5581 aattcctcct gcatccggac atagttccgc accgccccga aataattacc gaggtgtaaa
     5641 aagccggtgg gccggatgcc gctcataacg agttctttct ttgctgccat agggcggcga
     5701 agataagaat tagtcaggaa gtaagcccgg ccagggaaag cttaccgtta ataaaactca
     5761 tgcgcccggc tccggtacag ggattatctt tgcagcatgg aagtaaatga tcctttagtg
     5821 gaaaaactgg ctcatctctc ccggctgcag tttacggata ccgaaaaagc cggtatcaaa
     5881 aaagacctgc aacggatgat cggctttgtg gaaaaactaa atgaattgaa ccttgaaggg
     5941 gtggaacctc ttttgcatat gagcccggaa gtaaatgtgc tgcgggaaga tgagatccgt
     6001 ggttcagtca gccgcgaagc agccctccgc aatgccccgc tccatgacga acaatttttt
     6061 aaagttccca aagtgataaa aaaataatat ggcacagggc atcatccatc tggaagacat
     6121 acaaaagagc tattacatgg gtaaacaggc ccttccggtc ctgaagggca tttccatgga
     6181 tattttcaag aatgaatatg tggctttgat gggaccctcc ggttccggaa aaagtaccct
     6241 gatgaatatc ctgggttgcc tggatacccc tactgcgggt cgttatatcc tcaacaataa
     6301 tgacgtaagc accatggcgg ataatgccct ggctgaagtt cgtaataaag aaatcgggtt
     6361 cgtcttccag cagttcaacc tgctgccacg gctgacagcc gcagaaaatg tggcccttcc
     6421 cctggtttat gccggaatga ataaaaaatt acggaatgaa aaagccatgc aggtactgga
     6481 aatggtgagc ctgacagacc ggagtcatca caaacccaat gaactttccg ggggacagtg
     6541 ccagcgggtg gccattgccc gcgccctggt caatgatcct tccatcatcc tggccgatga
     6601 acctacgggt aacctggata ccaagacctc ctacgaaatc atggagattt tcaacacaat
     6661 tcacaataat ggcaatacca ttgtgctggt gactcacgaa gaagatatcg ccaatcatgc
     6721 gcacaggatt atacgccttc gtgatggtgt ggtagagacc gacaggaaaa acgaaaaggc
     6781 cggccaatat tcgcatcatt gatgcaataa atatctttac gggccgggaa catttctttg
     6841 agcaacattg ttgtgaagca cattcaacta acatggcaac caggatatac accaaagtag
     6901 gagacaaagg atcaacatca ctgataggcg gtaccaaagt gcccaaatcc catttacgta
     6961 ttgaagcata tggcacggtg gacgaattga attcggtcat tggtctttgt cgcgacctga
     7021 tcgtagaaga aaacagccgt gagattctcc gggaagtgca ggaccggctt tttaccattg
     7081 gttcttccct ggcctgtgat ccgattaagg aacccaaaat gcgccttccg gatctgaaag
     7141 aaacagatgt ggaattactg gaaaaagaaa tggaccggat gaatgaggaa ttgccggaga
     7201 tgaaaaactt tgttctgccg ggcggtcacc ccactgtttc ccatctccat attgcccgct
     7261 gtgtggcccg gagagcggag cgctgttgcg tacggctcga actggaaagc ctggaagtgg
     7321 aaccagtcat tcttaaatac ctgaaccggc tgagtgatta cctttttatc ctgagccggt
     7381 acaccggaat gaaactgaat gcccctgaaa taccctggaa acccagaatt taacagcctg
     7441 cttcagtcct gcatacaacc ctgttctggc ggatagcgac atatcatttt accttttttt
     7501 acgttttaac ttaaaccaga gatatgcgca acacctgttt ttttctcttt ttgttcattt
     7561 atctgagcag ttgccagcct gatccggtaa atcccccgaa taccggtccg ctccgggata
     7621 ccactttgtt taatgtcccc tacggttctg atcctgccca gaaactggac cttggcttac
     7681 ccgccaaccg aaccaataac agcgccctgg tgctggtcat acatggagga ggatgggcca
     7741 ccggtgataa agctgaactg aactggctgc tcaatgggtt aaaggccaga ggctttgccg
     7801 ttgccaatat caattaccgg cttaccctga atacaccgga taactacaaa atgcaactgg
     7861 atgatgtgga cagtgcacta aattataccc tgagacaatc gtccatacaa actttcaacg
     7921 gacaaaaatt atatctcgtc gggcatagtg ccggtgggca tttgtcgctt agttatgcct
     7981 atacccgtaa tagtaccggc cgcataaaag cagtgggctc tctggccggg ccaaccgacc
     8041 tgtatagcat gtcctactac aattttaata tatataacct gatacttgaa ccttacctgg
     8101 gtctaccctt attcccggcc agcgcagaag ctacagaccg gtataaagca tgcagtccat
     8161 tttatcaggc aacagctact gtgcccgcca gtattttctt tcatggagcg ctggatgtgg
     8221 tggttacacc ggatcagagc agttcgctta ataataagct gggaacactt ggagtggacc
     8281 ataaactggt cagttacccc ctcacttttc atgactggtg gactgatggc accaaagtga
     8341 ccaataccct tgatgaacta aagagctggt tcaacactca tccctgaacg ataatcccgt
     8401 cccgcatatg cagcacccgg tcactgaggg tagccagttc ctcattatgg gtcacaatca
     8461 gaaacgtctg gctgaactga ctccgcaggt caaaaaacaa ctgatggagg tctttggcat
     8521 tggctgaatc caggttgccg gtcggttcat cggcaaaaat aatatccggt ttattgatca
     8581 gggcccgggc gacagccacc cgctgttgtt ctccacccga tagctgattg ggcttgtttt
     8641 ccatccggtt agccagaccc agcataccga gcagtttttc ggcctcctgt ttcaccacag
     8701 ccttaccccg gcccgatagc cagccaggga tacagacatt ttccagggcg gaaaattccg
     8761 gaaggaggtg atgaaactgg aatacgaacc cgatatgttt attgcggaaa gccgccattt
     8821 ttttacctga cagactttgg agcaggacct ggttcatcct cacttccccg ctgtctgcgg
     8881 catcgagggt gcccagaata tgcaataagg tgcttttacc ggaaccagaa ggccccacga
     8941 ttgacaccac ttctccccgg ctgatttcca catccacccc tctcagtact tcgacggttc
     9001 cataccgttt atatatgttt tttccgctaa tcattcttca aatatcgcaa ataacggggg
     9061 ccggaaaaaa acaggttctc cggctttccc ccggttttat gaaaaccttt gaaaaccgtg
     9121 tacaattaag gatttggaaa attcattaat acaactacat ttgcggaaaa ttaaggggtt
     9181 ttgccgtaca agccggagcc cttatacttc ccgaaaaagg aagctttaaa caaaataaat
     9241 cctgagaaga tgttctggac acttgaatta gcctcgtacc ttgaagacgc accctggccg
     9301 gctaccaaag atgagctgat tgactattct atccgttccg gtgcacccat cgaagtggtg
     9361 gagaacctgc aggaactgga agatgaaggc gatgtttatg aaaccatcga ggatatctgg
     9421 cctgattatc caacgcagga tgacttcctc ttcaatgagg acgagtatta acccaaccgt
     9481 aaaagtatag aacccctttc tgaggggttt ttttatgccc gttcctaccc gtctatttta
     9541 tacttaccca ggtcagccgg attggtaatc ttgataaact ccatttttcc ccggttttca
     9601 ccacgggcaa ttttcaggaa aatatccaat gatttccaat actggtgatc ccggttggta
     9661 tcgagtacca gcaggtgtga catgataata ttgccggcca tttcaacgag gcgtcgggcg
     9721 tgaaaatcgg tgtattcatt attccccact tctgtcaccg ctttaaccgc ttcttcatat
     9781 tcattggtca tttcgctgat ataattccgg taaccgagca attcgggttt tacttccatc
     9841 tgttcgtagg ctttcatctg tgccaggtat cctccggttg ttacacctct cacagcagcc
     9901 actacctgca gttgcgtagt gccttcataa atactggtga tccgtgcatc ccggtaaatc
     9961 cgttcaatcg gatagtcttt cataaagccg gatccgccat gaatctgcag ggcatcatag
    10021 gctacctggt tacagacttc agaagaaaga ccttttgaca tcggggtaaa tatatccgcc
    10081 agtttctgat aggctttaag ctccgtccgc tcttccggtg tcaggcttct ttctttggaa
    10141 atatgctcat aggctttata cacatccaca aaccgggctg tctcatacag cagtgaacgg
    10201 atcgcatcca ccttcgcctt cattttagag atcatttcat acactgcggg aaacttactg
    10261 atggagcttc caaactgcac ccgctctttg gcataagaga cggcttcccg ccaggcagca
    10321 tcggcaatac ccacagactg ggcggcgatt cctaatctgg ccccgttcat gagtgccatc
    10381 acatatttga tcagtccgaa cttgcgttgt cccaccagtt cagccggtgc gtttttaaac
    10441 accagttcac aggtcgggga accaatgatt cccagtttgt gttcaatacg gcgaaccgta
    10501 accgcattac tgtcacggtc ataaataaac atggataatc cacgcccatc ggctgtccct
    10561 tcttcggacc gggccagcac taaggatata ttgccatctc cgttggtaat aaaacgctta
    10621 accccattga ggaaccattt cccatccacc agggttgctt ttaactgaac tgcctgcagg
    10681 tcacttccgg catccggctc tgtaaggtcc atggctgcag tctcaccagc ggcgatgcgg
    10741 ggcaggtaac gggcctgttg ttcctcatcg gcaaattcat ggatggtttc ggcgcaatcc
    10801 tgtaagcccc agatattgac aaaactcgca tctgccctcg ataccatatc ggccgccatg
    10861 atataaggca ctatcggaaa attcagtccg tcatatttac ggggcagggt gatgcccatc
    10921 aagcctgctt ttactatcgc ctccagattc ttctgggtgc cggccgcata tttcacatgt
    10981 ccgtcaatca gcagcggccc ttccgcatcc acactttcgg cattcggcgc cactgtattg
    11041 gcacaaatat ccccaacgat ctccagtact ttatcataac tatccattgc atcttcgaaa
    11101 tcaaccggtg cataatcaaa ttgttcgtgc gctgtatagt ttcgttcttt caacgccacg
    11161 attttctgca tcaggggatg ggtgaggtga aatttcagct cagtggtatc cgtataataa
    11221 tttgccatgt caactggttt ataaataatt gaatcaagtg gaacttttta cttgatattc
    11281 tttttatagt atctgatcat tttaggaatg atcgtttcca ggttgccctg aatcacatag
    11341 tccgccagtt tattaatcgg cgcattggca tcattgttaa tagatataac catggccgaa
    11401 tcctgcatac cggcaatatg ctggatctgt ccggagatgc cacaggcgat atacagtttg
    11461 gggcgaacgg tgataccggt ttgaccaatc tgccgttcct tatccgtaaa accggcatcc
    11521 acggccgccc gggaagcacc gacttcagca ccgagcgtgt gggccagttc ataaagcagg
    11581 ttaaaatttt cttttgaccc taccccataa ccaccggcga ttatgatgga actgttcttc
    11641 aggttcactt tcgacgtttc catatgccgt tcaataatct ttaccaccag gtcttcatca
    11701 cggaggtatt gcccggcatc caggtaaatg agttcggctt tatgtgtgtc agagattgtt
    11761 tcttttttca ttacgccttc cctgactgtt gccatctgcg gacggttatc cggatttaca
    11821 atcgtggcca cgatattacc accgaaggcg ggccggatct ggtaaagcag gttctcgtat
    11881 ttcttaccgg ctttcttatc ctcatattct cctatttcca ggttggtaca atctgcagtc
    11941 agtccgctgg taagtgagga agaaatccgt ggtccgagat cgcgcccaat cgaagtggct
    12001 cccatcagta cgatctgcgg atgggtttgt ttaaacaacc caactgccac tgcggtatga
    12061 ggcaatgtgg tatagggata aagccgttta tcatcggcca tatagaggat atcgacccca
    12121 tagcggctga cttctttttc caatccattc aatccttcgc ccaataccag tgcttccagt
    12181 ttacagttca gctgattggc cagtgaacgg cccttggtca gcagttcata gcttacttcc
    12241 tggatcctgc cttcttcaaa ttcgcagtac acgcaaacat tattcatggt gctgtttttt
    12301 atccttgatt agatgattat ccgattgtat gctgactgat gagttcctgc atcagcgatt
    12361 ctatctcgcc atcgtctgta ctgattacct tggattcctt ggcctggaaa acgatatttt
    12421 ccacctgtct gactttggta ggagaaccac ccagaccgag ttgcacgaga tcgctgttta
    12481 catccttggc acaccactcg gtgataagca cggcctgatc attgtgtaca gccaggtaat
    12541 cttcggctgt cgtgtgtcgt tcagatagcg tggtggctct tttaaaccgc attaaacgtt
    12601 ttgcattccg gggccggcat tccggggcgg tactatgtgc cgtgatcaat accggcaatg
    12661 tacattcaac gacttccaca cctcgttcca gccgacgttt tacctgtatg gtccggtcat
    12721 gaagtgaaat gatttcttct gcatacgtta tctggggaat ccccagttta tccgccacct
    12781 gcggacccac ctgggctgta tcgccgtcaa tggcctgtcg tccggccagg atcagatcgg
    12841 cttcaccgat tttgcggatg gcgcaggaca gcgcatacga tgtggccagt gtatccgatc
    12901 cggcaaaagc tttatcggtc agcaacacgc cggcatctgc tccccggtac atcccttccc
    12961 gcaggatttc ggctgcccgt acaggtccca tcgtcagcag ggtgatcttt gtgtccggat
    13021 actgatcctt gatctccagc gccagttcga gtgcattgag gtcttcgggg ttaaaaatag
    13081 caggtaaggc cgcccggttt acggtaccat cggccttcat tgcgtctttg cctacattcc
    13141 gggtatccgg aacctgtttg gctaaaacaa tgattttata catgtcccag taaggttgtt
    13201 gtggaactac aaaccttgga aatccctggg tgggaaaacc tgataaaaat cagggtaacg
    13261 aatgttttgg caccagaaag agaaagtacg aggtacgaag tacgaagtac gaaaaataca
    13321 aagtacgagg tacgagactt gaagcagata gcggactatc ggtctcgtaa ctcgcagctc
    13381 gtcactcgca gcttaaccct aaagctcacc tgctcataaa aaaaacaggg ttgcaccgct
    13441 cagtacaacc ctattctcta tttactattt tctatttcgc tcaaccttcc atcttctcaa
    13501 tcaccgcatg tgaaaccccg gtaaagctga accctccgtc atggaagaga ttctgcatgg
    13561 tgacaaaacg ggtgaggtcg ctgaaaaggg tgaccgtata gttggcacaa tcttccccgg
    13621 aggcatttcc gagcggactc attttttctg cataggaaat aaagccatca aatcctttaa
    13681 ccccgctgcc ggcggtcgtg cgggtgggtg actgggaaat agtattaatc cggactttat
    13741 ttcttacccc gtagtggtaa ccgaaattac gggtgatgct ttcgagcagg gccttggcat
    13801 cagccatttc gttatagtca gggaacaccc gctgagccgc aatataggtg agtgccacca
    13861 cgctgcccca ttcgctgatc gcatccatat tccaggccgt gtggagcagt ttgtgcagac
    13921 tgagggctga aatatccagt gttttatggt tgtgcgtata actgatctcc gtataatgat
    13981 gcccttttcg catattgata ctcataccta cagagtgaag gataaaatca attttaccgc
    14041 cgaaatgttt catggactcc tgtaataaat gtttgaggtc ctccaggagc gtgacatccg
    14101 caccgatgac cggggcattg ccacaaagtt cagacagttt attgatctcg cccatacgga
    14161 gcgctaccgg tgcattggtt aatacaatct gtgcgccttg ctcatgacat ttgaccgccg
    14221 tcttccaggc aatcgaattt tcatccagtg ccccgaaaat gattcctttc tttcctttga
    14281 gtaactgatt cgacataacg attggtttta tggcggtaaa aataaggaga tatgcggact
    14341 gaccctaaaa taatgatgag gtttcgcctg ctgttcacgc accggggaat agtaaccgga
    14401 tcccaaaaac aaggagaaac gtatagctaa aaactattac aaacataacc gccataaagc
    14461 caaaaaaatg ttgcagggaa tgacgcggct tcatttttac ctgcagccat ttatacaaaa
    14521 ggaataaggg caagagggag agcagaatta gtcccatgat catcagcgat ttcaatttca
    14581 tcgggttgtt taatttttta gacagaaaat gggctgaaaa gcctccggtc aatggtaaaa
    14641 taaacaagta gccctatctt agccggaaga aatcagccat tcctgatttt tcgtcaattt
    14701 atgcatatcc gcacgattat tactggctcc ggctgttata ttccacccgt gatcaaaacc
    14761 aaccgggact ttgctattca gactttctat ggtgaagaca ccaatcgtat tgaaaccccg
    14821 actgaagaga tcgtgacaaa attccaggat attaccggca tagaagaaag acggtatgca
    14881 gcagaagaaa tgaattcttc agatatggcc acactggctg cccgtcaggc catagaagac
    14941 agcgggatca atcccgaaga actggaccag atcattgtag cgcagaattt cgggaatgtg
    15001 atcaagcata cgatccagac tgatgtatta ccggccctgg cttcccgggt gaaacatgat
    15061 ctgggcataa aaaatccaaa ttgtgtggct tatgatatat tatttggttg tccgggctgg
    15121 gtacagggtg tcatccaggc tgatacctat ttcaaggccg gtgcggctaa aaaagcgctg
    15181 gtgatcggta ccgaaaccct gagccgtgtg attgacgtat atgaccgcga cagcatgatt
    15241 ttttccgacg gggcgggtgc cactattctc gaagcacagg acgtggatga aaaaggacct
    15301 ggtattctgt catggagtgt gcagagccat tgcctggacg aggcttacta tttgtacctg
    15361 ggaaaaagca attttcccgg aagcgatccc cggatccgct atattaaaat gaaaggccgc
    15421 aaggtctatg aatatgcgat gcagcatgta cctgctgcga tgaaggattg cctggataaa
    15481 gccggtgttt ctatatcgga tgtaaaaaag gtttttattc atcaggcaaa cgagaaaatg
    15541 gatgaaggga ttatccgccg tttattcaag ctgtacaata tcaaagaaat tcccgaacat
    15601 atcatgccca tgagtattca caaactggga aacagttccg ttgccaccat ccccacttta
    15661 tttgatatgg ttaaacgcgg actctgggat aatcatgacc tgaaggccgg ggatatcgtt
    15721 ctctttgctt ctgtgggggc tggcatgaat ataaacgcga tctgctaccg gatgtaagca
    15781 ctgtccggcc cgcacttcct cccgtttcca tatcttcgca gctttaaaag aagcaagcga
    15841 acatgacgat ttcctataaa tggctgagtg aatacctgcc agtaacagtg gaccctgaac
    15901 ggctgagccg tatcctgact tccatcggac tggaagtgga gagcatgcat ccctatgaag
    15961 aagtaaaagg cgggttgaaa ggactggtga tcggggaagt attacacact gaaaaacacc
    16021 cgaacgcaga taaattaacc ctgactaaag taaataccgg tgggacggaa cccctgcaga
    16081 ttgtgtgtgg ggcacccaat gtggccgccg gacaaaaagt agtggtcgcc accgtaggca
    16141 ctacaatcta cccttcagcc ggtgatcccc tgaccatgaa agtggcaaaa atccgcagtg
    16201 tggaaagcca tggaatgatc tgtgcggaag atgagatcgg tcttggcagt tcacatgcgg
    16261 gcatcctggt tttacccgcc gataccaaag tgggcaccct ggcggctgaa tacttcaagc
    16321 cgtatgaaga tatcatctac gaaatcggac tgacccccaa ccggatggat gcgatgagtc
    16381 attggggagt agcccgggat gtatgcgcct atctgagtca tcatgataaa aaagacctgc
    16441 ggcccaaaat tccccatagc aatggactga aggccgattc tctttccttc ccgatagaag
    16501 ttaaaattga aaacgatacc gcctgtcccc gttattcagg catcagcatt gacaaagtga
    16561 ccattgctga gtcacccgtc tggctgaaac agaaactgaa agcaatcggt ttgcgtccta
    16621 tcaataacat tgtggatatc accaattata ttcagcatga gacaggacaa cctctgcatg
    16681 catttgatgc agacctgatc cggggccgca aagtggtcat taagaattta ccggaaggca
    16741 ccccttttgt caccctggat gaaaaggaaa gaaacctcag tgccgatgat ctgatgatct
    16801 gtgatgaaga aggtggcatg tgcatcgctg gtgtgtttgg cggattacac agcggggtaa
    16861 ctgaaaaaac aaaaacgatt ttcctggaaa gtgctttttt cgaaggaatc gggttacgca
    16921 aaacctcgtt ccggcataac ctgcgtacgg atgcggccag ccgctttgaa aaaggaacgg
    16981 atatctccgc tacggtcaat gtactgaaac gtgccgcgtt gctgattaag gagatctgcg
    17041 gcggcgaaat cgcttctgaa atcgtggata tatatccaaa gaaaaaagaa aaggcggatg
    17101 tgcagatcaa atggcattac attaaaaaac tgagtggtaa aaattatcac ccggacagtg
    17161 tcaagaatat tctttccagt ttaggttttg aaataatcaa ggagggtatc gatgagttac
    17221 gggtggccgt gccgtttcac aaaccagaca tcagtcttcc cgccgatatc gtggaagaaa
    17281 tagtccggat tgatggcctg gacaatattg atattcctga atcgatcacc atcactcctg
    17341 ccgtggagga caattatgca agggaagtcg tgaaggaaaa aacggcggat tacctggtcg
    17401 gacttggcta ccatgaaatg atgaccaact ccattaccaa tgctgcttat ttttcagcgg
    17461 cggaactgga aaccatggtt accatgaaaa acagcctcag tgccgagctg aacatcctcc
    17521 gcagttctct ttttgaaacc gcgctggaag tggtggccca taacctgaat cacaaaaaca
    17581 gctcccttcg gttatttgaa ttcggtaaag catacagtac gtccggggcc ggaaaatacc
    17641 atgaagctga acagctttgt atactgatct ccggtaatag ccgggaagag gaatggaaac
    17701 aaaaggctgt cgccgctgat ttttatgccc tcaagggaac ggtggaagcg gttatgcgcg
    17761 ggctgggaat aaaagcagac agtgtggaag caatggaagt tcccaaactt gaaaaccata
    17821 tcgtttataa actgaataat catgtaattg caggagccgg acaggtcagt aaaggggtgc
    17881 tggatcaatt cggtataaag caaccggttt tttatgccgg cctgaactgg gaactgattt
    17941 cggatctggc gtccaatcat aatccgaaga tccgggagat cccccgttat ccgcaggtac
    18001 agcgggacct ggcgatgatt gtatcccggg aactgccctg gcaggaagtg gagaataccg
    18061 tgcataagat ccggctggat aaattacgcg acatcaaact ctttgatata ttcgagagtg
    18121 aaaaactcgg aaccggtaaa aaatcactgg ctgtgaattt tacattcctg gatgaggaaa
    18181 agacacttac cgacaaagaa atagatggct ggatgaataa gattatgggg gcactcgaaa
    18241 aagaattaca ggctgaaatc agaaaatgaa tacaaccgaa gaacagctga aacgaataca
    18301 ggataagctg cagcagttgc tgaagcaaca ggtggctgcg cgcagggaaa atgagaagct
    18361 gaaagaagag ctgggtgctg cccgtgaaaa gctggcaact cagcagaaaa gtacagatga
    18421 actgaaacaa caggtaactg tgctcaagat gaatgcaggg ggaatgaatg acatggataa
    18481 gaaggaactg gagaaaaaga tcaacagtta cctcaaagag atcgaccggt gtattgcttt
    18541 actgggagaa tgatcagacc cgtttgacca ggttcatttt catttcatca tcgccatgag
    18601 aaagttccag tgtataccgt ccataaggca ggtcatttaa tccggcccag gtcagttctt
    18661 ccatatcccg gaaaattttt tgctcaatga tgcggcatac tgaaccggtg tcgtctttca
    18721 acacaccacg cagggggtgg taccctttga tagcagtcaa ttggagttgt aatgcatcaa
    18781 taaaaactga tgacttaact ttagcgaaca tgggggaaag ttttggttcg ttatcatttc
    18841 ccactatagc cttaacctct taattttcag gagggtatac ggtgttcgat tgaggttaca
    18901 aagtaataaa acaattttga aattgcaagc aatggatgat ttaattctga tatcggccct
    18961 gatcggggac cgaacgtacc ggatcaagat agaaccccgg gatgaggaaa tggtccgcag
    19021 aaccctgaag accatcaatg aaaaaatcgt ggaattcaag acggagtttg ccggcaagga
    19081 tatgcaggac tatatcgcca tggtgatgat ctggctggct acggagatgc aatcggcccc
//
LOCUS       DAOPMW010000024        18743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2846_length_18743_cov_9.661548, whole genome shotgun sequence.
ACCESSION   DAOPMW010000024 DAOPMW010000000
VERSION     DAOPMW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18743
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2846_length_18743_cov_9.661548"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PLU11_02440"
     CDS             complement(<1..2004)
                     /locus_tag="PLU11_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN57995.1"
                     /translation="MKLFPDSAQVQLEFNKVRDLLALYCQSDHARAKAQELRVHTRRD
                     FIELELRQTHEYRQLSANSIYFPNEVILNLSKELKLLSIPGAMLTGEQFIAIRKLCEN
                     TAAIFRWFDNERRLAYPGLTKVINDTYYEKVIVELIDDVLDESGQVKDNASDDLRNIR
                     MNLYRKRNELRKVFERIVSKLNKQGYLAEIEESFMSGRRVVAVFAEQKRTVKGILHGE
                     SDSRKTAFVEPEETIELNNAVHELENDERKEVIRLLKELTAKLSVYAALLSTYHRVMG
                     EYDFIRAKARLAGDMNGEYPVIMDKAGVHLVEARHPLLYLYNRKLNKETVPVSVTLEE
                     SSRILVISGPNAGGKTVTMKTIGLLQLMVQSGLLVPVHPSSRFGIFKQLMIHIGDTQS
                     IEFELSTYSSHLLHMKDFITHANGRTLFFIDELGSGTDPNLGGAFAEVILQELVKKHA
                     IGVVTTHYLNLKVMAGKTPGIMNGAMSFDEKNLQPLYKLVLGKPGSSYTFSIAERIGM
                     DKQLINRARQLVDEDQYRLDKLLNRTEQDLQQLQKKEKQLSHTLKENEQIRKEMQQVL
                     EKEKHRQQVELLKEQNRVAEQKIAYMKDMERKLKQIMIEWRRAENKQKVIKQMEALLF
                     KKDETKVVGKMQKKIDEKFKEVGGEIRVGDKVKMKKNHQVGQVI"
     gene            2216..4795
                     /locus_tag="PLU11_02445"
     CDS             2216..4795
                     /locus_tag="PLU11_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPN57996.1"
                     /translation="MNRKALLILITTGTMLSAVAQNKQAAPSQPPAPTDSVKTKKPTG
                     IADKVKGSKKMEGLFTIYQDTVTGSVQLYIKKAQLGKEYIYQSFSISGPTQLFLNQSM
                     HRTTFVFKVQKAYDKLEFSRVNTSFYYDENNPVSKTKNIDKPEAVFLAEKSTGEDSAG
                     YLIQADGFFISEKMDAVRPANIPGMPMGMSFSLGGLNPLKSKYNQIRSYPGNTDVVVD
                     LAYDNPGAFVSGGPDITDPRYVRVRMQHSLIEMPSNNFRPRADDPRVGYFMQQVTDQT
                     SISPVPYKDMINRWHLEKKDPSAAISDPVEPIVYWVENTTPLEYRQTIVEAGLKWNAA
                     FEKAGFSNAVQMKIMPDDADWDPADIRYNVIRWVSSAQASYGAIGPSFVNPKTGQILG
                     ADITVEWFSGSNTPIIDELFSGAPANQPAPVFNIPGMDHQHLANCTMAQELKTQLMTG
                     MTVADITDAGPAEIKEMHKQFLYYLIMHEMGHTLGLNHNMKSSQMLSMAELNNKEITR
                     KQGLIGSVMDYPAINIASDKSKQGDYYTTLAGPYDIWAIQYGYTPFNAAEEEAGLRAI
                     LSRSTEPQLAFGNDGDDMRMPGKAMDPRVNVNDLSSDAIAYAEDRLKLVNSVMGKLVQ
                     KYSKPNQSFAELRARYGALQGQRNSMIAAASRYIGGIYIDRSTPDQRSGNKPYTPVPL
                     ATQKKAMELLSKYVFAPNAFDADNQVLPYLQPQRRGFNQSSTGDDYRITGVVLNQQGA
                     AVAHILHPATVQRITNSRLYGNQYSVADVMNDLVKACFEADLRTNVNVYRQYLQTAFV
                     KQAAFILDPKNPGYDDVARAAALNTLKKLKTQLAGAVSTNEETKAHRGNLVFLINNAM
                     EPR"
     gene            complement(4868..5281)
                     /locus_tag="PLU11_02450"
     CDS             complement(4868..5281)
                     /locus_tag="PLU11_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPN57997.1"
                     /translation="MTPEKISTTAFRLKPGEDLRAGIEKIVREKDIRAGWVATCSGSL
                     TDYAIRFANQPDASAGSGHFEIVSLTGTVSVNGSHLHLSISDSTGRTIGGHLTAGCKV
                     YTTAEIVLMYTDKYTFTREKDGSTPWEELQVHTND"
     gene            5422..6111
                     /gene="msrA"
                     /locus_tag="PLU11_02455"
     CDS             5422..6111
                     /gene="msrA"
                     /locus_tag="PLU11_02455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPN57998.1"
                     /translation="MKQRINLKPMLAVWMTGLVTLVACAQKAPGTTESKKEKQTMMNS
                     APLPATAAVATFANGCFWCTEAIFEELDGVISAVSGYTGGETISPTYKEVCDGNTGHA
                     ECLQITYDTSKISFDELLEVFWQTHDPTTLNRQGADVGTQYRSGIFYHNDEQKEKAES
                     YKAALDKSGAFDRPIVTEITPFSIFYPAEDYHQQYFELNGNSNPYCRVVIQPKLEKFR
                     KVFKDKLRKDR"
     gene            complement(6177..7421)
                     /locus_tag="PLU11_02460"
     CDS             complement(6177..7421)
                     /locus_tag="PLU11_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN57999.1"
                     /translation="MKTRLLAVVMTIVSFVIFSTEKSQAQAAPIFSFSSYSLESGTAL
                     SNGAVYRFSNVTTGVDALVTISAVTSGITLRNIDRTIDGYGEAFQPEYRINGNTNGYI
                     EFQIRFVNSGTSTTSNRPIVSATGLDIDGSPSGSNSLKEFNRIDMGGGSYEFNSYNAE
                     LTVSQSGTAVSGNNYTGNLFGALVDTTAKEVMFSVTNTNVGSMTYRIGSNNETSGSST
                     RYASLYYKKFTYQHFPLAISGLMSFDGVAVNNKVKLSWELAANKYSKVVLEKSNTSAN
                     FTKVYEANSGSFDHSGYTDADVQGGQLFYRLCATNLEGKTEYSSVIAVKVSGLVKNEM
                     NVYPSLIQTGATLAVALAEKTEGTVLISDLSGRIVKQQKVNLQAGSNSIAVDGFDNFA
                     KGNYIVALRTPAVVYSRKVVVQ"
     gene            complement(7658..9283)
                     /locus_tag="PLU11_02465"
     CDS             complement(7658..9283)
                     /locus_tag="PLU11_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPN58000.1"
                     /translation="MYRLTKYFPFLLAICLLSSCYNKTGQEKNIFHYNEFSGIPTLDP
                     AFAKSQATMWPAHQLFNTLVEIDDSLHIVPSLATRWDISADRTVYTFYLRNDVYFHDD
                     PAFPGGKGRKMKADDIVYSLSRIMDKRVASPGAWIFSRKTDTLQPFEALDDTTFRLNL
                     LYPYNPILGILSMQYCSIVPREVVEYYGNDFRRHPVGTGPFRMVAWEEGQALVLKRNE
                     KYFEKDSAGNPLPYLDGVKVSFYDSKATEFLLFRQKDLDFINDIEASFKDEVLTKAGT
                     LRKEWEGKIRLIASPYLNIEYLGILMDTTNPLLAGSPLRLKKIRQAINYGFDRRKMTL
                     YLRNSLGTPAESGFVPLGLPSFDSVVVRGYHFDPVKTRQLLAEAGFPGGKGLPVIKLL
                     TIAIYADMANFIAKQLEESGIPVQVEVVQKSLLLDMTSGSRAAFFRGSWIADYPDAEN
                     YLSVFYSKNPAPPNYTRYHNPAFDLLFEKAIAETNDSIRYELYRQADQLMINDAPVVP
                     LWYDKAVRLVQTRVVGFRANALNLLELRTTRFQ"
     gene            9346..11037
                     /locus_tag="PLU11_02470"
     CDS             9346..11037
                     /locus_tag="PLU11_02470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN58001.1"
                     /translation="MYRHSRILIVLPFVLISLFSGAQPLNKKETFTRQDTLRGSNGPG
                     RDWWNVIEYDIQITPDYSSKKITGSVQILFTVPQDCTDKIMQLDLQEPMRIDSMKQGN
                     EPLTEYRREGNVYFIDFGKRKFQKHQPAEQNYTFFSISVYFSGKPREAVNPPWEGGWI
                     WTKDNRGRPWMTVACQGLGASVWYPCKDYQGDEPDKGASLTVTVPDNLTAIGNGRCIN
                     KKAGKNGTTSWTWQVKNPINSYNIVPYIGKYVHFADRYTGETGEELSLDYWVLDVDSA
                     RAAKQFGRDVKRMLKAFEYWFGPYPFYEDGFKLVQSSHLGMEHQSAVAYGNHFMDGYL
                     GMDLSGSGWGKKWDYIIIHESGHEWFANNITSNDIADMWVHEGFTMYSEVLFTEFYYG
                     KAAGDAYLQGIRNNIANDRPLIGPYGVNKEGSGDMYNKGASLLHTIRQVIHDDSLFRK
                     IMRGLNQEFRHKTVDSKEIETYMSRVSGKDLSKIFDQYLRTTQIPELEYRLNGQELNY
                     RWTNVVKGFNMPLQLMGKPGSWLYPETGWKKITLPPGTGIEQYNPNFYITLKKGQ"
     gene            11081..12583
                     /locus_tag="PLU11_02475"
     CDS             11081..12583
                     /locus_tag="PLU11_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPN58002.1"
                     /translation="MSSIRRQSIISSLVIYIGFGVGLLNIYLFTRKGIFLEPQYGLYN
                     AFIAIATLMMAFSSFGMPTYIYKFFPYYKSHLPDKKNDQAAIALFTGIIGFIVIILAG
                     ILFKPLVIKKYITYAPEIITYYNWIFVLGFGLLIFTLLEAWAWQIHKSIFTNFLKELG
                     WRLFTMILILLFAAQLISFDLFIKLFAFSYPFIAVILLAYLLYTKQIHFNFKLSKVTR
                     RFSGSILKLSSFVYAALVVFNISIVFDSLLISSVMKDALAQLAVYSVAQNIAAMIQAP
                     QRGIISASMIHLATAWKEKNMEMIRKIYQRSSINQLLFACGFFSLVVLNLQDAVVTFE
                     LKDTYLKAFYVVIFLGMAKLVDMGTGVNAQIIATSTYWRFELFSGIILLSVMLPLSYI
                     LTREYGIVGTAVAQLISISIYNLIRVLFLWNKFRLQPFSRQTVYTILLGAACFALSYW
                     LFMDLHSWTGLFARSIFFCVVFAGGAIYMNLSPDIQPVLKNLKSRIGWKS"
     gene            complement(12587..13303)
                     /locus_tag="PLU11_02480"
     CDS             complement(12587..13303)
                     /locus_tag="PLU11_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPN58003.1"
                     /translation="MNFSGNKPVANLNETPAPAKTKFIEEITITPQADAEKIAAPDIW
                     EKPVVKQTAPAESKPAKKITDPAPVPTPVPASGNNLTSRGTPVVESATPVHFKYSVLM
                     DTEVESLPDKTLLDAVDEWYGVRYRVGGNTKSGVDCSGFTVAVYAAAYGMTLPRVSRD
                     QYRLSRKISTTELKEGDLVFFNTNGRGVSHVGVYLGNNKFIHASVSRGVMVSDLFENY
                     YLRRFVGAGRIDDKQIVSAE"
     gene            13638..14855
                     /locus_tag="PLU11_02485"
     CDS             13638..14855
                     /locus_tag="PLU11_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HPN58004.1"
                     /translation="MQILFPFFSFLILSTSCQQAPARQISMQSIIVEALPLDTIPQVL
                     QTAPKPYKSIIYEKKEVLKAAPGTKSVLFNSNGSKLYAMNLEGMSVYEFDQSTRKIVR
                     EFKFKPTRGTGWDYTKSKPIASFQEKPVEACFSHEDKILWVSLHNAEGIVPIRLDDLK
                     KNTANPAKGQLTKPVIVHYPGTAKKDSFLVPLIETGKTPKVIARSADSRHLLVSNWHS
                     YNVSILEMNETSSPFGKVIATVPVTAIPRGVVVDDVNKKSFVAIMGGSSLAVIDNTTW
                     KTDTILQVQNSPRHIVLDTSGHLFVSYNRIGKIACLDAKSGKSLFSTSTHAQPRTIAL
                     SKNHKFIFVTCYTSDFVDVYKINADNFEKVASLPCGGHPVGVDIFESENRLEAWVCSY
                     QKGEIVVYSFLKK"
     gene            15006..18680
                     /gene="dnaE"
                     /locus_tag="PLU11_02490"
     CDS             15006..18680
                     /gene="dnaE"
                     /locus_tag="PLU11_02490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPN58005.1"
                     /translation="MKFSHLHVHTQYSLLDGAASIKNLYKKAISDGMPALAISDHGNM
                     FGAFEFVAEAYRNKDENGNLKVKPVVGCEFYITENRHQKTFTKDQKDRRMHQILLAKN
                     EQGYRNLVKLTSLGFIEGMYSKYPRIDKELILKYHEGLIATTCCLGASVPQAILRKGE
                     EEGENEFKWWLNIFQEDFYVELQRHGMAEQDKVNEVLVKLARKYNVKIIASNDSHYVE
                     QDDFNAHDILLCINTGEKQATPALRDFADDDVSMKDRRFAFPNDQFYFKKTAEMSKVF
                     HDLPEAIDNTNEVVDKVELLSLTRDILLPNFPVEKRFEIHTKEETIGKYKVSAESQNQ
                     WEYLKHITYEGAANRYTEITDEIRERIDFELFTIKMMGFAGYFLIVSDFIKAGRQKGV
                     FIGPGRGSAAGSVVAFCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKV
                     IDYVVEKYGKNQVAQIITYGTMAAKMSIKDVARVMDLPLAESNALAKLVPDKPGIELK
                     RVLHAPMKAKEGEKSLEEKDGLGNDELESVKKLRDIYKQVDTPAGKVLHEAERLEGSV
                     RNTGIHAAGIIIAPQDLTDLIPVATSKDSHLWLTQIEGSVIEEAGVIKMDFLGLKTLT
                     IIKNALVLIKQNHGVEIDIDTIPLDDEKTFELYQHASTIGTFQFESPGMQKYLRDLKP
                     DKFGDLIAMNALYRPGPIAYIPNYIDRKHGREAISYDVPEMEEYLQETYGITVYQEQV
                     MLLAQKLAGFSKGDADVLRKAMGKKQKAVLDKMKAQFISGAKEKNLPEDKLEKIWTDW
                     EAFAQYAFNKSHSTCYAYVAYQTAYLKAHYPAEYMAAVLNNAGSIDKITFFMEECKRM
                     GLKVLGPDINESLKGFAVNEKGEIRFGLGGLKGVGEAAVESIIEERKKNGWFKNIFDF
                     IKRINQRTVNKKTLESLAYTGGFDCFPEHHRAQFFTIPEGETVNGLEKIIKFGQIMTA
                     QNASSTNTLFGDLPASMEIPPPRIPDCPAWSLVQQLDHEKEVAGIFLSGHPLDNYRFE
                     MKHYGVTTLTDFNEFKESIRMQPNPGRPFRIVGLVADAQHRIARSGNKFGIFVIEDYS
                     GKTEIALFSEDYMRLTPILQPGSTVLIHGYFKPRYNKEEFEFKLTAVSLAESMKKTMT
                     KQVTIEAHPGVFNTEMVSFIEKNMRDFPGKSTLRINLAEPRNKMKISLVTMSGGFEMN
                     EEMIQFLENKPELEVQVLTS"
BASE COUNT         5138 a         4363 c         4326 g         4916 t
ORIGIN      
        1 aatcacttgt cccacctggt gatttttttt cattttcact ttatcaccta cccggatctc
       61 gcctcccact tctttaaact tttcgtctat cttcttctgc attttaccaa ccactttcgt
      121 ctcatctttc ttaaagagga gtgcctccat ttgtttgatc actttctgct tattttccgc
      181 tctccgccat tcgatcatga tctgtttcag cttccgctcc atatccttca tataagcgat
      241 tttctgttca gccacccggt tctgttcttt tagtaattcc acctgctgac ggtgcttttc
      301 cttttccagt acctgttgca tttccttccg gatctgttca ttttctttca gggtatgact
      361 cagctgcttt tccttttttt gcaattgctg caggtcctgc tcagtgcggt tcaatagttt
      421 atcgagccgg tactgatcct catccactaa ctggcgggcc cggttgatca gttgtttatc
      481 cataccgatc ctttccgcaa tggagaacgt gtaggaactg ccgggcttcc ccagcaccag
      541 tttatataat ggctgcaggt ttttctcatc aaaactcatg gcgccattca tgatacccgg
      601 tgttttaccg gccatcactt tcaggttgag gtagtgggtg gtcaccacac caatcgcatg
      661 cttttttacc agttcctgaa gaatcacttc cgcaaaagcc ccccccaggt tcggatccgt
      721 tccactcccc agttcatcga taaagaataa agtccgtcca ttggcatgcg tgataaaatc
      781 tttcatatgc agcaaatgac tgctgtacgt actcagttca aattcgatgc tctgggtatc
      841 cccgatatgg atcatcagtt gtttgaagat gccaaaccgc gaagaaggat gcaccggcac
      901 cagcaaaccg ctctgcacca tcagctgcaa caacccgatg gttttcatgg tcaccgtttt
      961 accaccggca ttgggtccgc tgatcaccag tatccggctg gattcttcaa gggtaactga
     1021 aacggggacg gtttctttat tcagcttacg gttgtacagg tacaataacg gatggcgggc
     1081 ttccaccagg tgaacgccgg ccttgtccat aatcaccggg tattccccgt tcatatctcc
     1141 ggcaagacgg gctttggccc ttataaaatc atactcaccc attacccggt ggtacgtact
     1201 gagtaacgcg gcataaacgg ataatttcgc agtcagttct tttaacaacc ggatcacttc
     1261 tttccgttcg tcgttttcaa gttcatggac cgcgttgtta agttcaattg tttcttcggg
     1321 ttccacaaag gctgttttcc ggctgtcact ttccccgtgc aaaatccctt tcaccgttct
     1381 tttctgctcc gcaaacaccg ctacaacccg tctcccgctc ataaaacttt cttcaatttc
     1441 agcaagatat ccctgcttat taagtttgga gacaatccgt tcaaagactt tccggagttc
     1501 atttcttttg cggtacagat tcatacgaat gttccgcaga tcatcagatg cattgtcctt
     1561 gacctgtcct gattcatcca gcacgtcgtc aatgagttcc acaatgactt tttcataata
     1621 ggtatcattg ataacctttg tcagccccgg atatgccagg cggcgttcat tatcaaacca
     1681 tcggaaaata gcagctgtat tttcacagag tttgcggatg gcaataaact gctccccagt
     1741 cagcatggcg cctggaatcg ataacagttt taattctttt gagaggttca ggattacttc
     1801 attcgggaaa taaatactat tggcagacag ctgacggtat tcatgagtct gccggagctc
     1861 cagttcaata aaatcccgtc tggtatgcac acgtagttcc tgcgctttgg cccgggcatg
     1921 atcgctctga cagtaaaggg ccaacaggtc cctgaccttg ttaaattcca gttgcacctg
     1981 cgccgaatcg ggaaaaagct tcattctttg attttagtga accatcaatg gggagcaaat
     2041 ttcggatatt caacgcatat tgtcatgtct aaacagaccg gattcccggg cccttgacaa
     2101 tactatcccg gccggcccgc tctcatcccc caaaacttcc gttcatagaa atccttatct
     2161 ttctgatctt cctgtccctt attcttgtta gtcctttcaa attaaaaccc aacatatgaa
     2221 tagaaaagca cttctgatcc tgatcaccac aggtacgatg ctatctgctg tagcacaaaa
     2281 caagcaggct gcgccctctc agccaccagc tccgacagat agcgtaaaaa ccaaaaaacc
     2341 aaccggcata gctgacaaag tgaaaggcag taagaaaatg gaaggccttt ttacgatata
     2401 ccaggatacc gttaccggaa gtgtgcagct ttatattaaa aaagcgcagc tgggtaagga
     2461 atatatctac cagagctttt caatcagtgg gcctacccag ctgtttctga atcagagtat
     2521 gcaccggacg acttttgtat ttaaagtgca gaaagcctac gataaacttg aattttcgcg
     2581 ggtaaatacc tctttttact acgatgaaaa taatccggtc agcaagacaa aaaatataga
     2641 caaaccggaa gctgtattcc tggctgagaa atcaaccggc gaagattctg ccggatacct
     2701 gatccaggca gatggatttt ttatcagcga aaaaatggac gcagtgcgtc cagctaatat
     2761 cccaggcatg cccatgggca tgagtttttc cctgggcgga ctgaatccct taaaatcaaa
     2821 atacaaccag attcgttcct acccgggcaa taccgatgtg gtggtggacc tggcgtatga
     2881 taatccaggt gcgtttgtat caggaggacc ggatataacc gatccccgtt atgtgagggt
     2941 acggatgcag cacagcctga ttgaaatgcc ttccaataat ttcagacccc gggcagatga
     3001 cccgagggtg ggttatttta tgcaacaggt taccgatcag accagcatca gccccgtgcc
     3061 ttataaggat atgatcaacc gctggcatct ggagaaaaaa gatccttccg cagcaatcag
     3121 tgaccctgtt gagccaatcg tatactgggt ggagaatact acaccgctgg aataccgcca
     3181 gactatcgtg gaagccggcc tgaaatggaa cgcggctttt gaaaaagccg gtttcagcaa
     3241 cgctgtgcag atgaagatca tgccggatga tgctgattgg gatccggccg atatccgcta
     3301 caatgtgatc cgttgggttt cttcggccca ggcctcctac ggggccatcg gccccagttt
     3361 tgtaaacccc aaaaccggac aaatactggg agctgatatt acggtggaat ggttcagcgg
     3421 cagtaacacc ccgattattg atgaattatt cagcggggca ccggccaatc aacctgctcc
     3481 tgttttcaac ataccaggca tggaccacca gcacctggcc aattgcacga tggctcagga
     3541 actgaaaaca cagcttatga ccggaatgac cgttgctgat attacggatg caggaccggc
     3601 agagatcaaa gaaatgcata agcagtttct ctattacctg atcatgcatg aaatgggtca
     3661 tacgctcgga ctcaaccata atatgaaatc cagccagatg ctctccatgg ctgaactgaa
     3721 taataaagag atcacccgta aacagggatt gatcggatcg gtgatggatt atccggcgat
     3781 caatattgcc agtgataaat ccaaacaggg cgattattat accacgcttg ccgggcctta
     3841 tgatatctgg gccattcagt atgggtatac gcccttcaat gccgccgaag aagaggcagg
     3901 attacgtgct attctcagcc gcagtaccga accacaactc gctttcggga atgacgggga
     3961 tgatatgcgt atgcccggaa aagccatgga tccgcgggtg aatgttaatg acctcagcag
     4021 tgatgccatt gcctatgcag aagaccggtt gaaactggtt aattcagtaa tgggtaagct
     4081 ggtacaaaaa tacagtaaac cgaaccagag tttcgctgaa ctgcgggcgc gttatggagc
     4141 attgcaggga caacggaaca gtatgattgc cgcagccagc cgatatatcg gcgggatcta
     4201 tattgatcgc agtacacctg accagcgttc cggcaataag ccatacacac cggttccgct
     4261 ggccacccag aagaaagcaa tggaattgct cagcaaatat gtttttgccc cgaatgcttt
     4321 tgatgccgac aaccaggtac tcccctacct gcaaccccag cgccgtggat ttaaccaaag
     4381 cagcacaggt gatgattacc gcattaccgg ggtggtactg aatcagcagg gagccgctgt
     4441 tgcacatatc ctgcatccgg caaccgtaca gcggatcacc aacagccggc tgtatgggaa
     4501 tcaatacagt gtcgccgatg tgatgaatga tctggtgaaa gcctgttttg aagctgacct
     4561 gagaaccaat gtaaatgtgt accgtcaata cctgcaaaca gcctttgtta agcaagccgc
     4621 ctttattctt gatccgaaaa atccgggata tgatgatgtg gcaagggccg cagcactgaa
     4681 cacattaaag aaactgaaaa cccagctggc tggagctgtt tccaccaatg aagaaacaaa
     4741 agcacaccgc gggaacctgg ttttcctgat caataatgca atggagccca ggtagactat
     4801 cttagaattt ttgactcact aaaaaggctc tttggtttca aagagccttt ttttgagtca
     4861 atagtaatta gtcgttagta tgaacctgga gttcttccca gggggtggag ccgtcctttt
     4921 cgcgggtgaa cgtatattta tccgtataca taagaacgat ttcggccgtt gtataaacct
     4981 tgcaaccggc tgtcaggtgt ccgccaatgg ttcggccggt gctgtcgctg atactcaggt
     5041 gcaggtgcga gccattcaca cttacagtac cggtaagact tacaatttcg aaatggccac
     5101 ttccggcaga tgcatcgggc tgattggcga agcggatggc ataatcagtc aggctgcctg
     5161 agcaggtcgc cacccagccg gcacggatgt ctttttcccg gactattttc tcgattccgg
     5221 ccctgagatc ctcgccgggt ttgagccgga aagcagtagt actgattttt tcaggagtca
     5281 tggtcttggt tttacaggct gaaaaaagga taattgttaa aagaattaga acaatccgca
     5341 tgcggcatct gttttaatga ttggtccgta agttaatggg tattccgtca acctattcaa
     5401 taattcacga aatttaacca cttgaaacag cgaataaacc tgaagccgat gctggccgtt
     5461 tggatgacgg gactggtcac cctggtggcc tgtgcccaga aagcacccgg cacaactgaa
     5521 tctaaaaaag aaaagcaaac tatgatgaac tccgcgccct tacccgccac tgctgccgtg
     5581 gctacttttg ccaatggttg cttctggtgt actgaagcca tttttgaaga actggatggg
     5641 gtgatcagtg ccgtatccgg ttacacgggt ggcgaaacga tcagccctac atacaaggaa
     5701 gtctgtgatg gcaacaccgg gcatgccgaa tgcctgcaga tcacgtatga cacgtccaaa
     5761 atcagttttg atgaattact ggaagtgttc tggcagacgc atgaccccac cacactgaac
     5821 cgccaggggg ccgatgtggg tacccagtac cggagtggta ttttttacca caacgatgaa
     5881 caaaaagaaa aggctgaaag ctataaagca gcgttggata aaagcggggc atttgaccgc
     5941 ccgatcgtaa cggaaatcac ccctttcagt atattttatc cggccgaaga ttatcatcag
     6001 cagtattttg aattaaatgg taattcaaac ccttattgcc gggtggtgat acaacccaag
     6061 ctggaaaagt tccggaaagt atttaaagac aaactaagaa aagaccgttg atgtataaaa
     6121 aagaaagggc tgtatccgcg atacagccct tcttattcaa atgaaacccc tcttttttat
     6181 tgtacgacta ctttcctgga atatactact gccggcgtcc tgagtgccac aatataattc
     6241 cccttagcaa agttgtcaaa gccatcgacc gcgatactgt tagaaccagc ctgcagattt
     6301 actttctgtt gttttacgat ccgtccggac aaatcggata tcagcaccgt tccttctgtt
     6361 ttttcagcaa gagcaacggc caacgtggcc cctgtctgaa tcagtgaagg ataaacgttc
     6421 atttcattct ttaccaaccc acttacttta accgcgataa cactgctgta ttctgttttt
     6481 ccttccaggt tagtggcaca taaacggtaa aacagctggc caccctgcac atcggcatcc
     6541 gtataccctg aatgatcaaa tgaaccactg tttgcctcat atacttttgt gaaattggca
     6601 gatgtgttac ttttttccaa taccactttg ctgtatttat tggccgccag ttcccaggac
     6661 aatttcactt tattatttac tgccactccg tcaaaagaca tgagtccgga gatagccagg
     6721 ggaaaatgct gatacgtaaa ctttttataa taaagcgatg catatcgtgt actgcttccg
     6781 cttgtttcgt tattagaacc tatccggtaa gtcatgctac ccacattggt attcgttaca
     6841 gagaacatca cttctttggc cgttgtatct accagcgccc caaataaatt gccggtatag
     6901 ttattaccac ttaccgcagt accggactga gaaacagtca actctgcatt atacgagtta
     6961 aattcataag agccaccgcc catatcaatc cggttaaact ctttcagtga attgctgccg
     7021 ctgggactgc catcgatatc aagacctgtg gcagagacaa tgggcctgtt tgaagtagtg
     7081 ctggtgccac tgtttacaaa acgaatctga aattctatat atccattggt attgccattg
     7141 atccggtatt caggttgaaa agcttcacca taaccatcta tcgtacgatc gatattacgc
     7201 agggtgatac cggatgtaac agcactgatg gtaaccaggg catccacacc ggtggttaca
     7261 tttgagaaac ggtaaacggc accattactc agggctgtac ctgactctaa agaatatgaa
     7321 ctaaaactga aaatgggagc tgcctgtgct tgtgattttt cggttgaaaa gatgacaaaa
     7381 gatacaatgg tcatcaccac ggcgaggaga cgcgttttca taaaaggggt ttttgtttta
     7441 caaagggttg tgatcgttgg tcgctcgaag gatctggatt aatccggaaa tgttcccatg
     7501 aactttacgg agatattctg cttacctact ccgtatattt cggatacatc aaaagtaata
     7561 tgcacattta aaatatgcaa gcttttacga caaaaaatta atagtattta cttagtagat
     7621 ttacgtatac acatatacgt tactgaagat caatccctta ttgaaatcgg gtggtacgta
     7681 gttcgagcag gtttaatgca ttggcacgga aacctacgac ccttgtttgt accaatcgga
     7741 cggctttatc ataccataat ggcactaccg gggcgtcatt gatcataagc tgatcggcct
     7801 ggcggtagag ttcgtagcgg attgaatcat tggtttcagc aatcgctttt tcgaacaaca
     7861 ggtcaaaagc cggattatgg taacgggtat aattgggagg agcgggattc tttgaataaa
     7921 aaacagagag gtagttctcc gcatccggat aatcggcaat ccagctgccc cggaaaaaag
     7981 cggccctgga gccggaggtc atatctaata acagtgattt ctgtactact tccacctgca
     8041 ccggtatgcc ggactcttcc agttgtttgg caataaaatt ggccatatcc gcatagatcg
     8101 caatcgtgag cagcttaatg accggcaacc ctttcccccc cgggaaaccg gcttctgcca
     8161 gtaactgccg ggttttaacc ggatcaaaat gatagcccct gaccactact gagtcaaaag
     8221 aaggaagtcc gagtggcaca aacccggact ctgccggtgt acccaatgaa ttacgcaggt
     8281 agagggtcat cttacgccgg tcaaatccat agttgatggc ctgcctgatt ttttttaacc
     8341 gtaacggaga gccggcaaga agtggattgg tggtatccat taaaataccc aggtattcaa
     8401 tattaagata tgggctggcg atcagcctga tcttaccctc ccactctttt cgcagggtac
     8461 cggccttggt tagcacttca tccttaaacg atgcttcaat atcattaata aaatcaaggt
     8521 ccttttgccg gaataataag aattccgtgg ccttgctgtc ataaaaactc actttaaccc
     8581 catccaggta gggcaagggg ttacccgcac tgtctttttc aaaatacttt tcatttcgtt
     8641 tcaataccag ggcctgtcct tcttcccagg ccaccatcct gaacggaccg gtgccgaccg
     8701 ggtgccggcg gaaatcattc ccgtagtatt ccacgacttc ccggggaaca atagaacaat
     8761 actgcattga taaaatgccc aggatcgggt tgtacgggta aagtaaattg agccggaaag
     8821 tggtatcatc caaggcctcg aacggttgca gggtgtctgt tttccggctg aagatccagg
     8881 cacccggact ggccactctt ttatccatga tcctggacag actatacaca atatcgtcag
     8941 ccttcatttt tcgcccctta ccaccaggaa acgccgggtc atcatggaag taaacatcat
     9001 tcctgaggta aaaagtatat acagtccggt cggcggaaat gtcccagcgg gtggccaggg
     9061 aaggaacaat atgaagacta tcatcaattt ccaccagggt attgaatagc tggtgcgcag
     9121 gccacatcgt tgcctgactt ttggcaaagg ccgggtccag ggtcggaata ccggaaaact
     9181 cattgtaatg gaaaatattc ttttcctgtc ctgtcttgtt ataacaggag ctcagcaggc
     9241 agatcgccag taaaaaagga aaatattttg ttaaacggta catgagttaa taagagtcgg
     9301 actaaattag caaagtatcg cttataaaac gtttaaccgg aaagaatgta ccgacactcc
     9361 cgtattttaa ttgtgctccc tttcgtttta atatcccttt ttagtggcgc ccaaccgctc
     9421 aataaaaagg aaacattcac ccggcaggat acattgcgtg gcagtaacgg tcctggcaga
     9481 gactggtgga atgttattga atatgatata cagatcacgc cggattattc gtcaaagaaa
     9541 ataacaggca gtgttcaaat cctttttaca gtaccccaag actgtacgga caaaatcatg
     9601 cagcttgatt tacaggaacc gatgcgtatt gatagtatga aacagggaaa tgaacctctg
     9661 accgaatacc gaagagaggg aaatgtgtat tttattgact ttgggaaacg gaagtttcaa
     9721 aaacaccagc cagcggaaca gaactatacc tttttttcaa tttcagtcta tttctccggc
     9781 aagccccgtg aggcggtcaa tccaccctgg gaaggaggct ggatctggac taaagataac
     9841 cgcggccggc catggatgac cgttgcctgc cagggtttgg gcgccagtgt atggtatcct
     9901 tgtaaagact accaggggga tgagccggat aaaggcgctt cccttacggt taccgtacca
     9961 gacaatttaa cagcaatcgg gaatggacga tgtatcaata aaaaagcagg taaaaacgga
    10021 acaaccagct ggacctggca ggttaagaat ccaatcaaca gttataatat cgtcccttat
    10081 attggtaaat atgtacattt cgctgatcgt tataccgggg aaaccgggga ggaactttcg
    10141 cttgattact gggtcctcga tgtagattca gccagggcgg cgaaacagtt tggccgtgat
    10201 gtaaagcgta tgctcaaagc ctttgaatac tggtttggcc cctacccttt ttatgaagac
    10261 ggatttaagc tggtacaatc ctcgcacctg ggtatggagc accagagtgc ggtggcctat
    10321 ggcaatcatt tcatggatgg ttacctgggc atggatttat ccggcagtgg ctggggaaag
    10381 aaatgggatt atattatcat ccatgaaagt ggccacgagt ggtttgccaa taatatcacc
    10441 tccaatgata ttgcggatat gtgggtacat gaaggtttca ccatgtactc agaagtcttg
    10501 tttactgaat tttattatgg taaagcggcc ggtgatgcct atctgcaggg aatccgcaat
    10561 aatatagcca atgaccgtcc gctgatcggg ccctatggcg tgaacaaaga aggcagcggg
    10621 gatatgtata ataaaggcgc cagtctgctg cataccatcc gccaggtgat acatgatgac
    10681 agtctcttca ggaaaataat gcgcggactg aaccaggaat tcaggcataa aacggtggat
    10741 tcaaaagaaa tcgaaaccta tatgtcccgg gtttccggca aagacctttc taaaatattt
    10801 gaccagtatc tacgtaccac acagatccct gaactggaat accggttaaa tggccaggag
    10861 ctaaactaca ggtggacaaa tgtggtaaag ggattcaata tgcctctgca gctgatgggg
    10921 aagccgggtt cctggctgta tccggagacc ggctggaaaa agattacgtt accccccggc
    10981 accggtattg aacagtacaa tcccaatttc tatattaccc tcaaaaaagg acagtgaacc
    11041 tgatacctga caattccaat cagtccttta cctttaccaa atgagcagta tccggcggca
    11101 aagtattatt tcttcattgg tgatttatat cgggtttggc gttggcctgc tgaatattta
    11161 tttgtttacc cggaagggta tttttcttga accgcaatac ggtttatata acgcgtttat
    11221 agcgattgca acacttatga tggctttttc cagctttgga atgcctacct acatttataa
    11281 gtttttccca tattataaat cacatctgcc ggataaaaag aatgaccagg cagccattgc
    11341 cctttttacc ggtattatcg ggtttattgt aattattctt gccggcatcc tctttaaacc
    11401 ccttgtgata aaaaaatata tcacgtatgc cccggaaatc attacttatt acaactggat
    11461 ttttgtactt ggcttcgggc tgttgatttt tacactcctg gaggcctggg cctggcagat
    11521 ccataaatcc atttttacca actttctgaa agagcttggc tggcggctct ttaccatgat
    11581 cctgattctg ttgtttgcag cacagttgat ttcctttgac ctgttcatca agttgtttgc
    11641 attcagctat ccttttattg cagtcatctt gctggcttac ctgctctata ccaaacagat
    11701 tcattttaat ttcaaactca gcaaagttac ccgccgtttt tccggtagta ttctgaaact
    11761 tagttctttt gtttatgcag cactcgttgt ttttaatatt tccattgttt ttgattcatt
    11821 actgatcagc tctgtcatga aggatgcgct ggcccagctg gccgtttatt ccgtagccca
    11881 gaatatcgcg gcaatgatcc aagcccctca gcggggaatc atcagtgcct ctatgattca
    11941 cctggctaca gcctggaaag aaaaaaatat ggaaatgatc cgtaaaattt accaacggtc
    12001 atcaattaac caactcttat ttgcctgtgg ctttttttca ctcgtagttt tgaacctgca
    12061 ggatgctgtg gtcacttttg aattaaaaga cacttacctg aaagcatttt atgtcgtgat
    12121 cttcctggga atggccaagt tagtggatat gggaaccggt gtaaatgcgc aaatcattgc
    12181 cacttccact tactggcgtt ttgaattgtt tagcggcatt atcctgcttt cggtgatgtt
    12241 gccactgagt tatatcctta ccagggaata cggaatcgtg ggcacggcag tggcccagct
    12301 catctcgatc agtatctaca acctgatccg ggtacttttt ctctggaata aattcaggct
    12361 gcaacccttc tcccggcaaa ccgtatatac catactgctg ggtgcggcct gctttgccct
    12421 gagttactgg ctgttcatgg atcttcatag ctggaccggc ttgtttgccc ggagtatttt
    12481 cttctgtgtt gtttttgcag gcggggctat ctacatgaat ctttcacccg acattcagcc
    12541 agtattaaaa aacctaaaga gtagaatcgg ttggaaaagc tgaggcctat tcagcagata
    12601 cgatttgttt gtcatcgatc ctgcctgccc ctacaaaacg gcggagataa tagttttcaa
    12661 agaggtcact gaccatcacg cccctggaga cagatgcgtg aataaattta ttattgccca
    12721 ggtatacacc tacatgcgat accccccttc catttgtatt aaagaaaacc aggtctcctt
    12781 ctttcagttc ggtggtggaa atcttacggc ttaaccggta ttggtcacgg gaaaccctgg
    12841 gtaatgtcat cccataagcg gcggcatata cagctacggt aaatccggaa caatccactc
    12901 cgcttttagt attacctccc acccggtaac ggacaccata ccactcatcc accgcatcca
    12961 ataatgtttt atcagggagg gattccactt cggtatccat gagcacggaa tatttaaagt
    13021 gaaccggtgt agctgattcc acaactggtg tgccccttga agtcaggtta ttcccgcttg
    13081 ccggcacagg tgttggtacc ggagccggat ctgtaatctt tttagctggt ttactttccg
    13141 ccggggccgt ttgtttcacc actggctttt cccaaatatc cggtgctgct attttttcag
    13201 catctgcctg cggggttatt gtaatttctt caataaattt ggtctttgca ggtgcgggtg
    13261 tttcgttcag gttggcaacc ggcttgtttc cggaaaaatt catttgcttc agtgctgtac
    13321 agctgctgaa gaaaacgatc aaaaaaatga atgttattaa ttgcttaacc attggaaatg
    13381 ctgcaaaggt tgaattcata ggcggttaaa aaggtaatcg gtttgtaact cagcctctcc
    13441 ggagaaaggc cagcaaaaat atacgatatt cccctaaaat cggggtattc attaaacgaa
    13501 attaggcgct taaaattgcc ggaagcccta acccgctgaa aactaatcaa ctaaaatcca
    13561 atcctcaatt ttttttgtgt caaatcatcc gttaatcgga tgggttttat tgaatatcat
    13621 taacttatcc ttttattatg caaatactat tccctttttt cagtttcctc attttgagta
    13681 cgagctgcca gcaagcacca gcccgtcaga tttctatgca gtcgattatt gtagaagcct
    13741 tgccccttga tacgatccct caagtgcttc agacagcacc aaaaccatac aaatcaataa
    13801 tttacgagaa gaaagaagtc ctcaaagctg ctcccggcac caaatctgtc cttttcaaca
    13861 gcaatggctc aaaattgtac gccatgaacc tcgaggggat gagtgtctac gagtttgacc
    13921 agtccaccag aaaaatcgtc cgggaattta aatttaaacc cacccggggt acgggttggg
    13981 attatacaaa aagcaaaccc attgcttctt tccaggaaaa gccggtggaa gcctgcttca
    14041 gccatgagga taaaattctc tgggtttccc tgcacaatgc agaagggatt gttccgatcc
    14101 ggctggatga tctgaaaaag aatacagcca acccggctaa aggccagctg accaaacccg
    14161 ttattgttca ttatccgggc actgctaaaa aagattcctt tctggtgcct ttgattgaga
    14221 ccggcaagac cccaaaagtt attgcccgct cggctgacag ccggcacctg ttggtcagta
    14281 actggcattc ctataatgta agtattctgg aaatgaatga aaccagctcg ccatttggaa
    14341 aagtgattgc cacagtacct gttacagcca ttccgagggg tgtggtggtt gatgatgtga
    14401 ataaaaaatc atttgtggca atcatgggag gctcatcgct tgcggtcatc gataatacca
    14461 cctggaaaac agataccatc ctccaggtgc agaacagccc ccggcatatt gttttagata
    14521 ccagtggcca tttatttgtt tcctacaaca ggattggcaa gatagcctgc ctggatgcta
    14581 agtccggaaa gtcccttttc agcacctcca cccacgccca gcccaggacc attgccctgt
    14641 ccaaaaacca caaatttatt tttgtcacct gttataccag tgacttcgtg gatgtgtaca
    14701 aaatcaacgc cgataacttt gagaaagtgg catctttgcc ctgtggagga catcctgtag
    14761 gggtggacat ttttgaaagt gaaaaccggc tggaagcgtg ggtgtgcagt taccagaaag
    14821 gagagatagt cgtgtatagc tttctgaaaa aataactttg tttcagtcgg caggcagcag
    14881 ttggcagtca gcaaatggca ggtcgccttt gtcatcctga gcttgccgaa ggatgatcag
    14941 tagaaaacag ttggaagtcc ttcacggaat aatattgaat caacagaagt gagattgaaa
    15001 aggctatgaa gttttcgcat ttacacgtac atacacaata ctccctcctc gacggggcgg
    15061 cttcgataaa gaacctgtat aaaaaagcca tcagtgacgg aatgccggcg ctggccatca
    15121 gtgaccatgg taatatgttc ggcgcttttg aatttgtggc ggaagcttac cgcaacaaag
    15181 atgaaaacgg gaacctgaaa gtaaagcccg tggtgggttg tgaattctac atcaccgaaa
    15241 accgtcatca gaaaaccttt accaaggacc agaaagaccg ccggatgcac cagatcctgc
    15301 tggccaagaa tgaacagggc taccgcaacc tggttaaact gacatccctg ggttttattg
    15361 aagggatgta cagcaagtat ccccgtatcg acaaagagct gatcttaaaa taccatgaag
    15421 gactgatcgc caccacctgt tgcctgggtg catcggtgcc acaagccatc ctgcgcaaag
    15481 gcgaagagga aggggaaaat gaattcaaat ggtggctgaa tattttccag gaagattttt
    15541 acgtggaact gcaacggcat ggcatggcgg agcaggataa agtaaatgaa gtactggtaa
    15601 aactggcgcg gaaatacaac gtcaagatca ttgccagtaa tgactctcat tatgtggaac
    15661 aggatgattt caatgcccac gatattttac tttgtatcaa taccggggaa aaacaggcca
    15721 cccctgccct gcgtgatttt gccgatgatg atgtgagcat gaaggaccgg cggtttgcct
    15781 ttccaaacga ccagttttac ttcaagaaaa cggcggaaat gagcaaggta ttccacgacc
    15841 tgcctgaagc cattgacaac accaacgaag tggtggataa agtggaattg ctgagtctga
    15901 cccgtgatat cctgctgccg aatttcccgg tggaaaaaag atttgagatc catacgaaag
    15961 aggaaaccat cggaaagtat aaagtttccg ctgaatcgca gaaccagtgg gagtacctca
    16021 aacatattac ttacgaaggt gcggccaaca ggtataccga aataacggat gaaatccggg
    16081 agcggatcga ttttgaattg tttaccatca agatgatggg atttgccgga tacttcctga
    16141 tcgttagtga ctttattaaa gccgggcgtc agaaaggcgt atttatcgga ccgggtcgtg
    16201 gatctgcagc aggctcggtg gtggcttttt gtatcggcat caccaatatt gatcctatta
    16261 aatacaattt gctgtttgag cgttttctga atccggaccg gaagtcaatg cccgatatcg
    16321 atacggactt cgatgatgaa ggccgtcaga aagtgatcga ttatgtggtg gagaaatacg
    16381 gcaaaaacca ggtggcccag atcatcacct atggtaccat ggccgcgaaa atgagtatca
    16441 aagatgtggc ccgagtaatg gacctgcccc tggcggaatc caatgcgctg gctaaactgg
    16501 tacccgataa accgggaata gaattaaagc gggtgttgca tgccccgatg aaagcgaaag
    16561 aaggggaaaa gtcactggaa gaaaaagacg gattaggaaa tgatgaactg gaaagtgtaa
    16621 agaagctgcg tgacatatat aaacaggtgg atacacccgc cggtaaagta ctgcacgaag
    16681 cggaacgact cgaaggttcg gtccgtaata ccggtatcca tgcggcgggc atcatcattg
    16741 cgccgcagga tctgaccgac ctgattccgg tggccacttc caaagactcc catttatggc
    16801 tgacccagat tgaaggaagt gtgattgagg aagccggtgt aatcaagatg gactttctgg
    16861 gactgaagac ccttaccatc attaagaatg cgttagtcct gatcaagcag aaccatggag
    16921 tagaaattga tattgacacc atcccactgg acgatgaaaa gacttttgaa ctttaccagc
    16981 atgcttccac gattggtacg ttccagtttg aaagtccggg tatgcagaaa tacctccgtg
    17041 acctgaagcc cgataaattt ggtgacctga ttgcgatgaa tgccttatat cgtcccggtc
    17101 ccattgcgta tattcctaac tacattgacc gcaaacacgg acgcgaagca atcagctacg
    17161 atgtgccgga aatggaagag tacctgcagg aaacctacgg gatcacggtg taccaggaac
    17221 aggtgatgtt gctggcgcag aaacttgccg gcttcagtaa aggagatgcg gacgtgctgc
    17281 gaaaagcgat gggtaagaag cagaaagcgg tgctggataa aatgaaggct cagtttatca
    17341 gcggtgcaaa agaaaagaac ttacctgaag ataaactgga gaaaatctgg accgactggg
    17401 aagcgtttgc ccagtatgcc ttcaataaat cgcactccac ctgttatgcc tatgtggctt
    17461 accagacggc ctacctcaaa gcgcattacc cggccgagta tatggccgct gtgctgaata
    17521 acgccggcag cattgataag atcactttct ttatggaaga gtgtaagcgg atggggttaa
    17581 aagtattggg acctgatatc aatgaatcgt tgaaaggatt tgccgtgaat gaaaaaggtg
    17641 aaatccgttt tggactcggt ggcctgaaag gcgtgggtga agccgcggtg gaaagtatca
    17701 ttgaagaaag aaagaaaaac ggctggttta aaaacatctt tgattttatc aagcggatca
    17761 atcagcgtac ggtgaataaa aaaacactgg aaagtctcgc ttataccgga gggtttgatt
    17821 gtttcccgga acaccaccgg gcccagtttt tcaccatccc cgaaggagaa accgttaacg
    17881 ggctggaaaa aattatcaaa ttcgggcaga ttatgacggc ccagaatgcc agcagtacca
    17941 atacgttatt tggtgattta ccggccagta tggaaattcc cccgccccgg attccggatt
    18001 gtcctgcctg gtcgctggtg caacaactcg atcacgagaa agaagtggcg ggcattttcc
    18061 tgagcgggca tccgctggat aattaccggt ttgaaatgaa acattatgga gtaaccaccc
    18121 tgactgattt taatgaattc aaagagagta tccggatgca gcccaatccg ggccggcctt
    18181 tccggattgt gggcctggtg gccgatgccc agcaccggat cgcccggagt ggcaataaat
    18241 tcggaatttt tgttattgaa gattactccg gcaaaacaga gattgccctg ttcagcgaag
    18301 actatatgcg gctgaccccg attcttcagc ccggcagcac tgtgctgata cacggctatt
    18361 ttaaaccccg gtataataaa gaagaatttg agtttaagtt aacggcggtc agtctggcgg
    18421 aatcgatgaa aaaaaccatg accaaacaag taacgattga agcccatccg ggcgtattca
    18481 atacggaaat ggtcagcttt attgaaaaaa acatgcgtga ttttccgggc aaatcaaccc
    18541 tgcggatcaa cctggcagag cccagaaaca agatgaaaat cagcctggtg acgatgagtg
    18601 gcggctttga aatgaatgag gaaatgattc agttcctgga aaataagccg gaactggagg
    18661 tgcaggtact gaccagctga caggtactta gtacgatctt ttaacagctg ttaacaagtt
    18721 tccctgacaa gttttcaacc gtg
//
LOCUS       DAOPMW010000025        18179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_2963_length_18179_cov_11.096447, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000025 DAOPMW010000000
VERSION     DAOPMW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18179
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_18179_cov_11.096447"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PLU11_02495"
     CDS             <1..1806
                     /locus_tag="PLU11_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HPN58006.1"
                     /translation="KWIDLSSTTPDEALRTLLEDQLQVISVDYDPAAIDELTLPVPAV
                     VTRGLSFAGILNVPEAEVKVSLGYGTTITQTKTFILRKNETTDYDQVKRIWATMRISE
                     LELQFDKNKDAITRLGKEFSVVTQNTSLIVLDRVEDYVEQEILPPVELQKEYYALLKE
                     KKEQENTNKGYALQSALNAMQELKNWWSRDYKPDVKKNQSADTGTDQVFSMVEREGSM
                     ANGLVVTDSARVSTEYFTAPVILADEKVSLPPPAQEQLQASAIGNTESMKMAEENADG
                     VMDKNEEFKEKSQSTIDVKEWKPDVPYLKELEKTSARDFLSKYLNLKKAYVDQPSFYI
                     DVARFFFQQKEKQLAIQVLSNVAEMKLEEASLMRMMANQLMEAGEKELAVESYRDILK
                     MREEEPQSYRDLALALNETGAYNEAVQLMYKLVLGTWHGRFADVKSITLNEMNAIISA
                     HPGRVDLSGIDPRLVYAMPVDVRIVISWNADNSDIDLWVTDPVKEKCFYQNSATSLGG
                     RISQDVTGGYGPEEFMLKKAVNGKYKVEVNLYGDNRQTLGGPIAIKADLFTDYGKPTQ
                     KRETINFRVTTGKEVVELGALSFAAESMAGNNSHK"
     gene            complement(1999..3267)
                     /gene="purT"
                     /locus_tag="PLU11_02500"
     CDS             complement(1999..3267)
                     /gene="purT"
                     /locus_tag="PLU11_02500"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HPN58007.1"
                     /translation="MIPHACWCFHQHAPPCWSSDQHVLVGENTNKYLRPMHKKILLLG
                     SGELGKELVIALKRLGQTVIAVDSYAGAPAMQVADGYEVIDMLNGQELDKLVFDYKPD
                     LIVPEIEAIRTERFYEYEKQGLKVIPSAKAANFTMNRKLIRDLAAKELGLRTANYFYA
                     TQFEEFNKAVDSIGLPCVVKPLMSSSGKGQSVLKSDEDRQKVWDYAINNSRGDIKEVI
                     IEEFIAFHTEITLLTVTQQNGPTLFCPPIGHRQERGDYQESWQPVAISDKDYNEACEM
                     AGKVTTALTGSGIWGVEFFLTDKGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAI
                     LGLPIPEIKLERAGASAVVLANKSAEQFTIHGIDNALAQPDTDIRIFGKPSTRPYRRM
                     AVALVYDQPGADMDTLRKKAKEVADMIRVD"
     gene            complement(3264..4331)
                     /locus_tag="PLU11_02505"
     CDS             complement(3264..4331)
                     /locus_tag="PLU11_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58008.1"
                     /translation="MKAVLKRFRIKISHWEYWPTELVYGPIFPVFLWYCLRARSFFFT
                     TASNPGIQHGGLMMESKYSIDAVVPAEYRPHSLYFEPGTAFSTVESALYNHQLSYPVI
                     VKPDIGGKGMGVIKVENARHLADVISQFPVPYLVQPFIDLPLEIGLFWARLPGQETGT
                     ITGIVRKEFLQVTGDGKQTVRSLLMANDRYLLQLPALEKLLVDKLEQIIPDGITELLV
                     PYGNHARGALFLDESRLISPALIQRFNRVCNQIEGFYFGRLDIRYKNWASLEKGLDFS
                     IIELNGAGSEPTHIYDPGHSLFFAWKEIIRHWKLLWIISMENKKQTKTSWMSLKEGLH
                     MLKSHFRYLKKCKHGFKLMKQ"
     gene            complement(4336..4920)
                     /locus_tag="PLU11_02510"
     CDS             complement(4336..4920)
                     /locus_tag="PLU11_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58009.1"
                     /translation="MPPSLINPFEYSSILVSIILGLGITQLLSAFSDLLYNYKKVSFY
                     WPQTLWLVFILFLHIQDWFITYQLKDKPEWHFAELVFILLYPVSLFMAAKLLLPSNDK
                     EESRQMKSYYMSQYPVLFAVMAVSILLSVIFNIWLLREPLLAQVHLLMFFLVILLIAV
                     LKVRHPMIHKFTAIAVCVAALTSLIIETESWVIK"
     gene            complement(4924..5691)
                     /locus_tag="PLU11_02515"
     CDS             complement(4924..5691)
                     /locus_tag="PLU11_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPN58010.1"
                     /translation="MCTVLFIPSNGRYLFVSLRDEDPFRAKANNPQIYEGENYRYLAP
                     VDPKAGGTWAGINSYGNVMILLNGGFEKHKPAGQYRKSRGLIVKELLQSVYPVVEWSL
                     MDMGGIEPYTLITWSENNLFQLVWDGHSKHRMLLDTQQPAIWSSATLYTPEAKEKRSQ
                     LFDQWVHDQPFIDHKTVMKFFQSFKDYENGFLMHRSASLRTLSYSFIELQPSVQAIYH
                     YDDFSGSLTDEQIIHTSGYSPDLSVLFSNASIAPCKK"
     gene            complement(5727..6224)
                     /locus_tag="PLU11_02520"
     CDS             complement(5727..6224)
                     /locus_tag="PLU11_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58011.1"
                     /translation="MIQYQLQDQLQGLVRLLQQLTDEQYTRKIRHLGQATIGGHSRHI
                     IEIVQCVLSGYETGRVDYIHRYRNTELEMNRSAAIQLIQEVERMLNKTDRSLELFVET
                     ATGVLQNSPLTTYLREIIYSIEHTVHHLALIKVALTEMELSLADETFGIAYSTMLYRK
                     SIAKG"
     gene            complement(6243..6656)
                     /locus_tag="PLU11_02525"
     CDS             complement(6243..6656)
                     /locus_tag="PLU11_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPN58012.1"
                     /translation="MTIKNAVTWLLRIVAAVILLQTLYFKFTGHPESVALFTKLGVEP
                     WGRIGTGVIELVAGILLLLPATVFIGAFLGIGLMSGAILSHLTIIGISSGDDGGQLFI
                     MAILVWVICLILLLLFLPQGKSLLQSMKPIISKKG"
     gene            complement(6670..7125)
                     /locus_tag="PLU11_02530"
     CDS             complement(6670..7125)
                     /locus_tag="PLU11_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HPN58013.1"
                     /translation="MKKYLFAACLLLTISGFAQTALRTKTFNLEKGIAIQGYDPVAYF
                     TQNKAVKGKKELSVFAEGATYYFSSQTNKDLFLKDFRKYEPQYGGWCAYAMGASGEKV
                     EIDPDTFKIVNGKLFLFYHSWINNTLTKWNKDEINLKSKADRNWQLLYK"
     gene            complement(7143..7580)
                     /locus_tag="PLU11_02535"
     CDS             complement(7143..7580)
                     /locus_tag="PLU11_02535"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HPN58014.1"
                     /translation="MTKKVVSTLVTLLFTGLVIGQPVKYNSKNGIAIKGFDPVAYFTL
                     GKAVKGLPEISFNWSGSTWLFSTKAHLDSFSKHPEKYAPQYGGYCAYGCSENHLSPTD
                     PEAWTIINNKLYLNYNLRTRVVWLQDTVARIKNADIHWPVLNH"
     gene            complement(7661..8515)
                     /locus_tag="PLU11_02540"
     CDS             complement(7661..8515)
                     /locus_tag="PLU11_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN58015.1"
                     /translation="MKYIFTHPDGHGRLLLHKNENSFQQYFYKQQAGFTFHTIAWNKG
                     KKQLIYIDEVAWDFEENAVLPIMFNQTFRFERPADIVAWQFNREFYCIVNHDAEVGCV
                     GFIFFGPSPTMFIRLDPADATEYTRLMQVFETEFDSDEEIKGEMLRMLLVQLIIKTTR
                     LAKKQYLGTAEPTEDKFNLIRQYNLHVEIHFRKEHQVQFYAGLLHKSPKTISNYFALY
                     SKKKPLQIIHDRLIMEARRLFTYTDKPIKEIANELGFEDVSHFSKFFKKYTSLNPSDL
                     RKSLINSK"
     gene            complement(8632..>9030)
                     /locus_tag="PLU11_02545"
     CDS             complement(8632..>9030)
                     /locus_tag="PLU11_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPN58016.1"
                     /translation="AHGNEPSHHVAWLYAWSDKPELTSRLITRICNEFYTDKPTGMIG
                     NDDCGQMSAWYIFATLGLYPVNPANGLFIRSLPQCNKIVLDINESAPVVIRRSGTLKQ
                     PEYWLNNTLIKENFIPFSALRRLAGNGYSR"
     assembly_gap    9031..9130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9131..10446)
                     /locus_tag="PLU11_02550"
     CDS             complement(<9131..10446)
                     /locus_tag="PLU11_02550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPN58017.1"
                     /translation="MNKIIPLCFFWLLNVTCLAQVKQATDYTRFVNTFIGTEGTGHTF
                     PGPSMPFGLVQPGPDNRDNGWDYTSGYQYKDSLIMGFSQTRANGTGISELGDILLQPV
                     TDERTDDFAISYRKETEKASPGYYTVELKNKVKTELTATERVAFHQYTYPDHKAKLLI
                     DLQHGLRFLTDSLVLESNVQVENNSRLSGWCHTQNWVNRKYYFTIEFSLPFTTILELP
                     KKAKENASRFLLGFMLKDKRLKVKIALSTVSVEGAKENLKTELPHWKFNTIVSLAKKT
                     WNKYLSRIVIEAPVKQKTIFYSCLYRLLLQPSNICDTDGQYRGPDDKIHTALNKEYYS
                     TLSLWDSYRAAHPLYTLIVPERVNGFVNTLVEHAKTAGFLPIWTVWGQDNYCMIGNHA
                     IPVIADAYLKGFTGFDAEEALTQMVRSSRENHINSNWTLLNQYGYYP"
     gene            complement(10443..>10776)
                     /locus_tag="PLU11_02555"
     CDS             complement(10443..>10776)
                     /locus_tag="PLU11_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58018.1"
                     /translation="IRFEAAIQKNQTNGDEYYFQYGPLVLCHPLNSTEVITKTYTIAG
                     LTDFNCLPLKETVVELNGALPVQDATVKNHFHAEMKPASTDQPEVILLVPMAGTTLRQ
                     VTFKMKGQ"
     assembly_gap    10777..10876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10877..11950)
                     /locus_tag="PLU11_02560"
     CDS             complement(<10877..11950)
                     /locus_tag="PLU11_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPN58019.1"
                     /translation="MKKNYLTLLLIALAMLQNINLPAQDSSANPFLKIKPLAFRFQAL
                     PLSAITPGGWLKTELKRNLDGFTGRLDTLAPDLIQQDDIYGKDRLSKKVKNKNLGAVS
                     DAGEWQAQFLWWNSETQSNWLDGFIRTAILLKDKTQLEKAERLINRLLQTQDKDGYIG
                     IYDPELRYRFDNENGELWSKATLYRALLGWYDYKKDPALLTAIEKAVKNVMDNYPVNQ
                     SHPFYSVNPDAGGLTHGLAFTDVLEQLYVLTKKQAYLDYCLFLYKDFSTQTLNEDAQF
                     KKLQQADLPLKGHGVHTYEHLRSVIAAWYASGNPALKSAIDQFIRKINRATTPSGAPV
                     GDEFAGGRAGDATSTGYEFCSLHE"
     gene            complement(11947..12786)
                     /locus_tag="PLU11_02565"
     CDS             complement(11947..12786)
                     /locus_tag="PLU11_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58020.1"
                     /translation="MEIKWLCPYWGQFPAEATVFTDRAIGAGYDGIEIHLPDDKKFRS
                     ALLSELEIIAGDHPGFVFVAQQVLSAAHETVSDYISRMISSLKDIALLQPDFINAHTG
                     KDYFSFDDNCRVIDTVLNFSQQSGIKVFHETHRGRFSFHLAGLLPYLKQFPEIKLTGD
                     FSHFCVVSESLLEDQQEQLESITPHIAHLHARIGFSQAPQVNDPFAPEWAATLDTYTG
                     WWERILQYRKKAGDKQFTITPEFGPVPYMPVLPFTQQPVGDQWEINKKMKDRLSERFN
                     KLL"
     gene            complement(12791..13618)
                     /locus_tag="PLU11_02570"
     CDS             complement(12791..13618)
                     /locus_tag="PLU11_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPN58021.1"
                     /translation="MSAIPSFVSSFLKEDYALSKEQISFYQQNRFIKLKQVFSPEVLN
                     WLNEVITERVRSMNKETRSLDERNTYGKAFLQLFNLWLEDEQVSELVLSRRLGKIAAE
                     LMQCEGVRLYHDQALFKEAGGGFTPWHADQYYWPLQTDKTITAWIPLQPTPLEMGPLE
                     FSAGSHAVIEGRELEIGDDSETMIRERLKVTDFAHVIEPFDLGEMSFHSGWVFHRAGA
                     NNSQDMRKVMTIIYMDRDMRLKAPENKNQQLDWDTWCPGAQIGEVINSKINPIIYQH"
     gene            13739..14608
                     /locus_tag="PLU11_02575"
     CDS             13739..14608
                     /locus_tag="PLU11_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN58022.1"
                     /translation="MRPKLEKVSAGNGSFLSFEVRQLSFDFFWHFHPEYELTYIVSGQ
                     GKRLVGDSVETFGPGDFVLIGPGLPHVWVSEKKTDQLAVALVVQFSAAFVESVLSFPE
                     LSAADSLFRKAQQGICFQVARHWKPERKMKVIAGLQPGNPFTVLLELLQELTVKKSRT
                     LASAQYKLLRSGNDGRRIHKVLKFVQDHYREPITVSQAAAMLHLSDSAFCKYFKRSMG
                     KTFSDFVNDIRITQAASLLMETDLSIAALAADCGFENISYFNRVFLRKKGIQPYKFRE
                     SRRENRKYNMPND"
     gene            14621..15232
                     /locus_tag="PLU11_02580"
     CDS             14621..15232
                     /locus_tag="PLU11_02580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN58023.1"
                     /translation="MKYTIIALLLFLSACSVSKNPLRKQVKFLQKGIIKDDTSYIYAL
                     PYEEGKTFRVIQGYFSRFTHKERAALDFNMKRGTKICAAREGVVVRVKEDGKKGGLDK
                     KYRSDGNNIVIQHPDGSRAGYWHLQYNGALVNVGDTVKKGQVIALSGKTGYAFNPHLH
                     FLVWRSSGGNWQQVPTRFMTSKGVKYLRGWRRYTVGENTNSRR"
     gene            complement(15397..16224)
                     /locus_tag="PLU11_02585"
     CDS             complement(15397..16224)
                     /locus_tag="PLU11_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58024.1"
                     /translation="MKQSLFVLLFSFSFFFQYSHAQVTTVKSPAQNQPKLKMPAGLPA
                     KIKQVDSLKKAHFSAGQILKTYIPKYGPQFQQEMTENLIVMYHSGFKTGELADGVKTQ
                     YPEIRFPVVFRIFYTALKEIRTYGNSTTWIFTSSDTLKISYGLPVERGYVDYPHFYIK
                     TLKDGGASVSDIFYFINKYMVTGSPANWQNIENNVKALLQSGFKEDVVLKNLLIIRER
                     AAWIFTGFCKGAKSAGVDAVTTSRVLNMENLTKDEISNYLKNGGYTAAEILAALQAL"
     gene            complement(16256..17038)
                     /locus_tag="PLU11_02590"
     CDS             complement(16256..17038)
                     /locus_tag="PLU11_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58025.1"
                     /translation="MRIIGLLFLLGLSCYTTAQSVRLPQGVTMDTLPEGFSEGLFRHH
                     LYKNKQVIASIVPVTWHNNDTRGTTIATSYEASFLVGTVRKDSFTTVKQSLQWLVPVI
                     EDYFSPVDYPRWKSLRIQPPKVRIRYPWEWSYKSDRNNSIFQSKAQTNNRLALLSTNN
                     SEIMQVIRTPNTGKLTLDQLILLSQRMNPAINLQQNPLQDILIGGKTFKTTNHTFMEL
                     MLQQHYWYADEKEIIYIGVGLLREDQVRYPLVIKEILKSIKW"
     gene            complement(17069..17896)
                     /locus_tag="PLU11_02595"
     CDS             complement(17069..17896)
                     /locus_tag="PLU11_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN58026.1"
                     /translation="MKRLALLILVSISLLSSAQDKRGCDTIEPKYMTRMPGFLLENCD
                     LSEFSTYKFSYIDLKDKRIYKEVEGRFYRLRYSKIKGESRKFSGEQIRFNYHNAVVKA
                     KGSSLSLRKDFFKMNQNGKTVWFKIDNAEDSDDQGFDLFIVETEEMKQDIEVNMSEGI
                     DRDGKIALYGILFDVGKSVIKPESAPALQMVIDYLIANPGIKIYVVGHTDNTGLFASN
                     ITLSKARAKAVKDYLVTKGKIAATRLGSDGVGSLCPVATNGTEEGKALNRRVEIVKQ"
     gene            complement(17925..>18179)
                     /locus_tag="PLU11_02600"
     CDS             complement(17925..>18179)
                     /locus_tag="PLU11_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58027.1"
                     /translation="AEAGVLSVEASATQFISFDSDFNVTDVGNKLSVGASAMEISSTL
                     GLEGLSASGDVTFGVNSGLNVSSSASAMGQSMFAGEIQLL"
BASE COUNT         4743 a         3946 c         4222 g         5068 t
ORIGIN      
        1 aaatggatcg acctcagcag tacaaccccg gatgaagcgc ttcgtacatt actcgaggat
       61 cagttgcagg tgataagcgt ggactatgac ccggcggcca ttgacgaact gacattgccc
      121 gtacccgctg tagtgacccg cggactttca tttgccggta tactgaatgt accggaggcg
      181 gaagtaaaag tttcactggg ttatgggact actatcacgc aaacaaaaac attcatcctg
      241 cggaaaaatg aaactacgga ttacgaccag gtaaaacgga tctgggccac gatgcggatc
      301 agtgaactgg agctgcagtt tgacaagaac aaagatgcga tcacccgcct gggtaaggaa
      361 ttttcggtgg tcacgcaaaa cacttcattg atcgtgctgg accgggtgga ggattatgta
      421 gaacaggaga tcctgccacc ggtggaatta cagaaagaat attatgcatt gcttaaagag
      481 aaaaaggaac aggaaaacac taacaaaggc tatgcgctgc aatcagctct gaatgccatg
      541 caggaattaa agaactggtg gagcagggac tataagccgg acgtaaagaa gaatcagtca
      601 gctgatactg gaactgacca ggtgttttca atggttgaaa gggaaggatc aatggcaaat
      661 ggattggtgg taacagattc agcacgggtg tcaacagaat attttactgc tccggtaatt
      721 ctagctgatg aaaaagtgtc ccttccccct ccggcacagg aacagttgca agcctcagct
      781 atcggcaata cagaatctat gaagatggca gaggagaatg cagacggggt gatggataag
      841 aatgaggaat ttaaggaaaa aagccaatcc actattgatg tgaaggaatg gaaacctgat
      901 gtgccttatt taaaggaact ggagaaaaca agtgcccgtg attttctcag taaatacctt
      961 aacctaaaaa aggcatatgt ggatcagcct tcgttttata ttgatgtggc ccggtttttc
     1021 ttccaacaga aagaaaagca actggccata caggtactga gtaatgtggc tgaaatgaag
     1081 ctcgaagagg cttcactgat gcgcatgatg gccaatcagc tgatggaagc aggggaaaaa
     1141 gaactggcgg tggaaagcta tcgggatata ctgaagatga gggaagaaga accgcagtct
     1201 taccgtgacc tggccctcgc cctgaacgaa accggggcct ataatgaagc cgttcagctg
     1261 atgtataaac tggtattagg cacctggcat ggccgtttcg ccgatgtgaa gtccattacg
     1321 ttaaatgaaa tgaatgcgat tatcagtgcg catccgggtc gtgtagactt gtctggcatc
     1381 gatccgcgac tggtttatgc catgccggtg gatgtacgga tcgttatcag ctggaatgcc
     1441 gataacagcg atattgatct ctgggtcacg gatccggtca aagaaaaatg tttttatcaa
     1501 aactcagcta cttcattggg tggacgtatt tcacaggatg taacgggagg ttatggtccg
     1561 gaggagttca tgctgaagaa agccgtgaac ggaaagtata aagtggaagt gaatttatat
     1621 ggggataatc gccagaccct tggaggaccc attgcgatca aggccgatct gtttaccgat
     1681 tatggaaagc cgactcagaa aagggaaacg atcaatttcc gggtcacgac cggtaaggaa
     1741 gtggtggaac tgggggcgtt gagttttgcg gctgaaagta tggcggggaa taatagccac
     1801 aaataaagaa gtacattatg ctactcatgc ttcggcaagc tcagcatgac aggtgcagcc
     1861 cgttgtcacc ctgagcctgc ctaccggaca ggcaggcgtg tcgaagggtg agtagctaat
     1921 gcttcggcag gcttagcata acatgaacag cagctcaggt tatttatagt tggtgttatc
     1981 accagcaatt ttcatcgctc agtccacccg aatcatatcc gctacctctt tcgccttctt
     2041 ccgtagtgta tccatatcag cccccggctg atcatatacc agagccaccg ccatgcgccg
     2101 gtagggtcgc gtggatggtt ttccgaatat acggatatct gtatccggtt gtgccagggc
     2161 attgtcgatt ccatgaatgg taaactgttc agctgattta ttggccagca ccacggcact
     2221 ggcaccagct ctttccagtt tgatctccgg gatcggtaag ccaagtatgg cccgggcatg
     2281 cagttcaaat tcggaaaggt tttgtgtacc ggccagggtc accatgccgg tatcatgcgg
     2341 acgcggactg agttcagaga aataaacacc tttatctgtt aagaagaatt ccactcccca
     2401 gataccggag ccggttaagg cagtggttac tttaccagcc atttcacagg cttcgttata
     2461 gtctttatca ctgatcgcca ccggctgcca gctttcctgg taatcgccgc gttcctgtcg
     2521 gtggccgatg ggcggacaaa ataaagtggg tccgttttgt tgcgtcacgg tgagcaaggt
     2581 gatctcggta tgaaaagcaa taaactcctc gatgatcact tctttgatat cacctcttga
     2641 attattaatt gcatagtccc atactttctg ccggtcttca tcggatttga gcacggattg
     2701 tcctttgcca ctggaggaca taaggggctt caccacacag ggcagtccga tagaatcgac
     2761 ggctttatta aattcttcaa attgtgtagc atagaaataa ttggccgtac gtaatcccag
     2821 ttcttttgcg gccagatccc ggatcagttt gcggttcatg gtgaagtttg cggctttggc
     2881 actggggatg acctttaatc cctgtttttc gtattcataa aaacgctctg tgcggatcgc
     2941 ttcaatctcc ggaacaatca ggtcgggttt ataatcaaat accagtttat ccaactcctg
     3001 cccattgagc atatcaatca cttcatagcc atcggccacc tgcatggcgg gagcgccggc
     3061 ataactatcc acggcaatca cggtttgacc aagccgtttt aacgcaatca ctaattcttt
     3121 accgagttca ccagagccga ggaggaggat ttttttgtgc atggggcgaa gatacttgtt
     3181 ggtgttttca ccaacaagta catgttggtc agaagaccaa caagggggtg catgttggtg
     3241 aaaacaccaa caagcatgcg gtatcactgc ttcatcagtt taaatccgtg cttacatttt
     3301 tttaaatagc ggaaatgact tttgagcatg tgaaggcctt ctttcagact catccagctg
     3361 gtcttggttt gttttttgtt ttccatgctg ataatccaga gcagtttcca atgccggata
     3421 atttctttcc aggcaaaaaa cagcgagtgt cccgggtcgt agatatgtgt tggttcactg
     3481 ccggcaccat tcaattcaat aatactgaag tccagccctt tctccagtga tgcccagttc
     3541 ttatagcgga tatccagtct gccgaaataa aatccttcaa tctggttaca gactcgatta
     3601 aagcgttgaa tcaatgcggg gctgatcagc cggctttcgt ccaggaacaa ggcaccgcgg
     3661 gcatgattgc cataaggtac aagcagttct gttattccgt caggaataat ctgctccagt
     3721 ttatcaacca gtaatttttc aagagctggc aattggagca ggtaccggtc attagccatt
     3781 agtaaagaac ggacggtttg tttcccatca cccgttactt gcaaaaattc tttccggaca
     3841 atacccgtta tggtgcctgt ttcctggcct ggaagtcgtg cccagaataa accaatttca
     3901 agtggcagat caataaatgg ctgcaccagg taaggaaccg gaaattgact gattacgtcg
     3961 gctaagtgcc tggcattttc cacttttata acgcccattc cttttccccc gatatcgggt
     4021 tttacaatca ccggatacga caactggtga ttatacaatg cagattcgac tgtactaaat
     4081 gcagtacctg gttcaaaata caaggagtgg ggccggtatt cagcgggtac aaccgcatcg
     4141 atactgtatt tactttccat catcagcccc ccatgctgta tacccggatt cgaagcggtg
     4201 gtaaagaaaa aactccgggc tctcaggcag taccaaagga ataccgggaa tatgggtcca
     4261 tataccagtt cggttggcca gtactcccag tgactgattt taatccggaa tctttttagt
     4321 actgctttca taaaattatt ttattaccca gctttctgtt tcgataatca gtgaagtaag
     4381 agcggcaacg caaacagcaa tggccgtgaa tttatggatc atcgggtgac ggactttcag
     4441 gacggcaatc agtaagatta ccaaaaaaaa catgagcagg tgtacctgtg caagcaaagg
     4501 ctcccggagc agccagatat taaaaataac agaaagcagg atgctgaccg ccatgaccgc
     4561 aaaaagcacg ggatactggc tcatataata ggacttcatt tggcggcttt cttctttatc
     4621 atttgaaggc agcaagagtt tggcggccat gaaaagcgaa accgggtaaa gcaaaataaa
     4681 gacaagctcc gcgaaatgcc attccggttt atcttttaat tgataggtga taaaccagtc
     4741 ctgtatatgc aggaaaagaa taaagaccag ccaaagcgtt tgtggccagt agaatgacac
     4801 ctttttataa ttgtataata aatccgagaa agccgagagt aactgagtaa ttccaagtcc
     4861 cagtatgatg gaaaccagga tgctgctgta ttcaaacgga ttgatgagcg aaggaggcat
     4921 aaattatttt ttacaaggcg caatcgatgc atttgaaaaa agaacggaga ggtcggggga
     4981 atagcccgag gtatggatga tttgttcatc cgtcaaggag cctgaaaagt cgtcgtaatg
     5041 gtaaattgcc tgaacggatg gctgtaattc aataaaagag tagctgaggg ttctcagtga
     5101 tgctgaccgg tgcatcagga aaccattttc atagtcttta aaagactgga agaatttcat
     5161 aaccgtttta tggtcaataa aaggctgatc atgtacccac tgatcaaata actggctgcg
     5221 cttttctttt gcctcagggg tataaagtgt agcggaagac cagatggcgg gttgctgggt
     5281 atccaggagc atccggtgtt tgctatggcc atcccaaacc aactgaaaca ggttgttttc
     5341 agaccaggtg atcagtgtgt acggttcaat tccccccata tccatcaacg accattccac
     5401 taccgggtat acggattgca acaattcttt tacgatgagc ccccggcttt tccgatactg
     5461 accagccggt ttatgttttt caaacccgcc attcaggagg atcatcacat tcccatagct
     5521 gttgatcccg gcccaggtac ctccagcctt cggatctacc ggggccaggt atcggtaatt
     5581 ctctccttca tagatctgcg ggttattggc ctttgcccgg aacggatctt catccctgag
     5641 tgaaacaaat aaataacggc cattggaagg aataaataag actgtacaca ttttcagttt
     5701 attttgatga taccatcatg cagcaactat cccttggcaa tggatttcct gtacagcata
     5761 gttgaatagg cgatgccaaa ggtttcatca gccagcgaca actccatttc agtcagggct
     5821 actttgataa gggccaggtg gtgaacggta tgctctatcg agtagatgat ctccctcaga
     5881 taggttgtca acggactgtt ctgaagaacg ccagttgctg tttccacaaa caattccagg
     5941 gacctgtctg ttttattcag cattctttct acttcctgaa tcagttggat cgctgctgac
     6001 cggttcattt ccagttcagt atttctgtat cggtgtatat agtccacgcg gcccgtctca
     6061 tatccggata atacgcattg aacgatttca ataatgtgcc ggctgtgtcc gccaatggtg
     6121 gcttgcccca ggtgcctgat cttacgggta tattgttcat cggtaagttg ttgtaatagc
     6181 ctgaccaacc cttgcagctg gtcttgaagt tgatactgta tcatgcagtg catatttcag
     6241 ggttaccctt ttttagatat gatcggtttc attgattgca acagagattt tccttgtggc
     6301 agaaaaagca gcagtaagat caggcagatt acccaaacca ggatcgccat aataaacaat
     6361 tgccccccgt catctccgga gctgataccg attatagtga gatgagaaag gatagcgccg
     6421 gacatcaatc caattcccag gaaagccccg ataaaaacgg tagctggtaa aagcaggagt
     6481 atgcctgcta cgagttctat cacgccggta ccgatccgac cccagggttc cacacccagt
     6541 ttagtgaata aagccactga ttcagggtgt ccggtaaatt taaaataaag tgtctggagt
     6601 aagataacgg ctgctacaat ccggagcaac caggtaactg catttttaat agtcatgttg
     6661 attgatttat tacttatata aaagctgcca gttccggtca gcctttgatt tcaggtttat
     6721 ttcatccttg ttccatttgg tgagggtatt attgatccag gagtgataga acagaaacaa
     6781 tttaccattt acaattttaa aggtgtccgg atcgatttcc actttttcgc ccgatgcccc
     6841 catggcataa gcacaccagc ctccgtattg tggttcgtat tttctgaaat ctttcagaaa
     6901 gagatcctta tttgtctggc tggaaaaata ataggtagct ccttctgcaa atacactcaa
     6961 ttccttttta ccctttaccg ctttgttctg cgtaaaataa gctaccgggt catagccctg
     7021 tatggctatc cctttttcca ggttaaaggt ctttgtccgg agtgcggttt gtgcgaaacc
     7081 ggaaatggtc aacagcagac aggctgcaaa tagatatttt ttcattttac ttatttttca
     7141 gattagtggt tcaataccgg ccagtggata tcggcatttt tgatacgggc tacagtatcc
     7201 tgaagccata caactcttgt cctgaggttg tagttcaggt agagtttgtt atttatgatc
     7261 gtccaggctt cagggtcggt gggcgacagg tgattttcac tgcatccgta tgcacaatac
     7321 cctccgtatt gcggggcata tttttcgggg tgttttgaaa agctgtccag gtgcgcttta
     7381 gtggagaata accaggtgct tccgctccag ttaaaagata tttccggcag tccttttacc
     7441 gcttttccga gggtgaaata ggccaccggg tcaaagcctt ttattgctat tccgttttta
     7501 ctgttgtact taacaggctg gccgattacc agccctgtga aaagcagggt gaccagggtg
     7561 ctgactactt tttttgtcat ttgttttatt tgcagcaaaa atgctttttg cacggctgct
     7621 aagcaattga catatttccc tgtttcaggt atatttttcc ctatttgctg tttatcaggg
     7681 atttcctgag gtcagaggga ttgagcgagg tgtatttttt aaagaactta ctgaaatggg
     7741 aaacatcctc aaatcccagt tcgttggcga tttctttgat gggtttatcc gtataggtaa
     7801 acaaacgtct tgcttccatg atcagcctgt cgtgtataat ctgtaagggt ttctttttgc
     7861 tgtacagggc aaaatagttg gagatcgttt ttggtgattt atgtaacaga ccggcataaa
     7921 actgcacctg gtgttccttc ctgaaatgga tttcgacatg caggttgtat tgcctgatca
     7981 ggttgaattt atcttcggtg ggctccgccg ttcccaggta ttgttttttt gccaggcggg
     8041 tggttttgat aatcagctgc acgaggagca tccgcagcat ttctcctttg atctcttcat
     8101 cggagtcgaa ttccgtttca aacacctgca taagtcgtgt atactctgtt gcatcggccg
     8161 gatccagtcg tataaacatg gttggcgagg ggccgaaaaa aatgaaacca acacaaccca
     8221 cttcagcatc atggttgaca atgcaataaa attcccggtt aaattgccag gctacaatgt
     8281 ctgccggtct ttcaaaacgg aatgtctggt taaacatgat gggtaagacc gcgttttctt
     8341 caaaatccca ggcgacttcg tcgatataaa tgagctgctt tttcccttta ttccaggcaa
     8401 tcgtatgaaa ggtgaacccc gcttgttgtt tgtaaaagta ctgctgaaac gaattttcgt
     8461 ttttatgtaa aagcagccgg ccatggccat ccggatgggt aaaaatgtat ttcatattat
     8521 ttcttgcaga ataataccta cggataacaa ttgaagacag ttttaatctg aattgttaaa
     8581 aggcgtctgc cttttcccga aaataggaaa caaacgaatt caggcctcaa attaccggct
     8641 gtaaccattg ccggccaaac gtctgagagc agaaaaaggg ataaaatttt ctttgatcag
     8701 ggtgttattc agccagtatt caggttgttt cagggtgccg cttctcctga tcacaacagg
     8761 agctgattca tttatgtcta agacaatttt attgcattga ggtagcgaac ggataaatag
     8821 cccgtttgcc ggatttaccg ggtagagtcc caaagtggca aaaatatacc aggcactcat
     8881 ttgaccacaa tcatcattcc caatcatacc cgttggtttg tcagtataaa attcattgca
     8941 aatgcgggta atgagccggg aggtaagttc gggtttgtca gaccaggcgt atagccaggc
     9001 tacatgatga ctgggttcat ttccatgtgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn ggataataac catattgatt caacagggtc cagttactgt tgatatggtt
     9181 ttcccggctg cttcttacca tttgtgtcag ggcttcttct gcatcaaatc ctgtaaaacc
     9241 cttcaggtac gcatctgcaa ttaccggaat agcatgattg ccgatcatac agtaattatc
     9301 ctgtccccaa acggtccaga tgggcaggaa tcctgcagtt ttagcgtgtt ccaccagggt
     9361 atttacaaaa ccgtttactc tttcaggtac aatcagggtg taaagcggat gggctgcacg
     9421 gtaagaatcc cagagtgaaa gggtggaata gtattcttta ttcaatgcag tatggatctt
     9481 atcatccggt ccgcggtatt gcccgtcagt atcgcagata ttggagggtt gcagcaataa
     9541 ccggtacagg caggaataaa aaatagtttt ttgtttaaca ggtgcctcaa ttacgatccg
     9601 gcttaaatac ttgttccagg ttttttttgc aagacttaca attgtattaa atttccaatg
     9661 gggcagttcg gtcttcaggt tttcctttgc accttccaca ctcacagtgg ataaggcaat
     9721 ctttaccttc agtcgtttgt ctttgagcat aaaacccaac aggaagcggg acgcattttc
     9781 ttttgctttt tttggcaatt caaggatggt ggtaaacggc agactgaact cgatcgtaaa
     9841 gtagtattta cggttaaccc agttttgggt atgacaccac ccggacaacc ggctgttgtt
     9901 ttccacctgc acattgcttt ccagtaccag gctatccgtc aaaaaccgca agccatgctg
     9961 caggtcgatc agcagtttcg ctttatggtc gggataagta tattgatgaa aggccacccg
    10021 ttctgtagcc gtcagctctg tctttacctt gttttttaat tcaactgtat aataaccagg
    10081 agaagctttt tccgtttctt tccggtagga tattgcgaaa tcatccgtgc gttcatccgt
    10141 aaccggctga aggaggatat cgcccaactc actgatgcct gtaccattgg cccgcgtttg
    10201 tgaaaagccc atgatgaggc tgtctttgta ttgataccca ctggtataat cccagccatt
    10261 atcccgattg tctggtccgg gctgcactaa cccaaaaggc atggatggcc ccggaaaagt
    10321 atgccctgta ccttcagtac ctataaaagt gttcacaaaa cgcgtatagt ctgtagcttg
    10381 tttaacctgt gcaaggcagg ttacgttcaa caaccagaaa aaacaaagag ggatgatttt
    10441 gttcattgcc ctttcatttt aaaggtgacc tgtcgcaggg tggtaccggc cattgggaca
    10501 aggagaatca cttcgggctg atcggtgctg gctggtttca tttcagcatg aaaatgattt
    10561 ttaacagttg catcctgcac gggtaaagca ccattcaatt caacaaccgt ttcctttaac
    10621 ggaagacagt tgaaatcggt taaacccgca atggtgtatg tttttgtaat tacttcagta
    10681 ctgtttaaag gatgacaaag taccagcgga ccatattgaa agtaatattc atctccattg
    10741 gtttgattct tttgaatggc tgcttcaaaa cggatgnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnttca tggagcgagc agaactcata gccggtactg gtagcgtctc
    10921 cggctcttcc accggcaaat tcgtcaccca ccggtgcacc ggaaggggtg gtggcccggt
    10981 tgatcttccg gataaactga tcaatggctg atttcagtgc gggattaccg gatgcatacc
    11041 aggctgcaat tacagaacgc agatgctcat aggtatgcac gccatgacct tttagcggaa
    11101 gatcagcttg ctgcagtttt ttaaattgtg catcctcgtt gagtgtttgt gtagaaaaat
    11161 ctttgtatag aaagagacag taatccaggt acgcttgttt tttggtaagc acgtataatt
    11221 gttcaagcac atctgtaaat gccagtccat gtgtaagtcc tcccgcatcc ggattaaccg
    11281 agtaaaatgg atgggactgg ttcaccggat agttgtccat tacattcttt acggcttttt
    11341 caatggctgt taacagggcc gggtcttttt tatagtcata ccatcccagc agggcacggt
    11401 aaagtgtggc ttttgaccag agctcgccgt tttcattatc aaacctgtac ctcagttcgg
    11461 gatcatagat accgatatac ccgtctttat cctgtgtttg cagtaagcga ttgatgagtc
    11521 gttcggcttt ttcaagttgt gttttatcct tgagcaggat ggcggtacgg ataaatccat
    11581 ccagccagtt actctgtgtt tcactgttcc accagagaaa ctgggcctgc cattcaccgg
    11641 catcagatac tgcccccagg tttttgttct ttactttttt actgagccgg tcttttccat
    11701 agatatcatc ctgctgtatc aggtccggag ccagggtgtc aagacggcca gtaaatccat
    11761 ccaggttacg ctttagttcc gttttcagcc agccgccagg agtaattgca gataagggca
    11821 atgcctggaa ccggaaggcc aatggcttta tttttaaaaa gggattggca ctgctatcct
    11881 gtgccggtaa gttgatgttt tgaagcattg ccagcgctat taacaacagg gttaaataat
    11941 tttttttcat agcagcttat taaatcgttc actcaacctg tctttcatct ttttattgat
    12001 ttcccattga tcaccgaccg gttgttgggt aaagggtaag accggcatat agggaaccgg
    12061 tccgaactca ggcgttatgg taaattgttt atcgccagct tttttccggt attggaggat
    12121 gcgttcccac cagccggtat aggtgtccag tgtagctgcc cattccggtg caaacggatc
    12181 atttacctgt ggagcctgtg agaaaccgat ccgggcatgc aggtgggcga tatgcggggt
    12241 aatggattcc agctgctcct gctggtcttc gaggagactt tcactgacta cgcagaagtg
    12301 actgaagtcg ccggtcagtt tgatttcagg aaactgtttg agataaggga gcagaccggc
    12361 caggtgaaaa ctgaaccgtc cacggtgtgt ttcatgaaac acttttattc cggattgctg
    12421 actgaaattt aaaacagtat caatcacccg gcagttatca tcaaaactga aatagtcttt
    12481 gcccgtatgg gcattgataa aatcaggctg caggagtgcg atatccttca ggctactaat
    12541 catccggctg atataatcgc ttaccgtttc atgggcagca gacaaaacct gttgggcaac
    12601 aaatacaaag ccggggtggt ctcccgcgat gatctccagt tcgctcagca gggctgatct
    12661 gaatttttta tcatcgggca ggtgaatttc aataccatcg taaccagccc cgatggcccg
    12721 gtcagtaaat actgttgctt cagcagggaa ttgtccccag taaggacaga gccattttat
    12781 ttccatctgt tcaatgttga tagatgattg gatttatttt actattgatc acttcaccaa
    12841 tttgtgcacc cgggcaccag gtatcccaat ccagttgctg gtttttattt tcgggggctt
    12901 tcagccgcat atcccgatcc atgtagatga tggtcataac tttccgcata tcctgactat
    12961 tattcgcacc cgcccgatga aagacccaac cggaatgaaa actcatttca ccgaggtcaa
    13021 agggctcaat tacatgggca aaatccgtta ctttcaaacg ctcccggatc atcgtttcac
    13081 tatcatcccc gatttccagt tcacggcctt caatcactgc atgactgccc gcactgaatt
    13141 cgagcggacc catttccagt ggagtgggtt gaagggggat ccaggcagta atggttttat
    13201 cagtttgcaa gggccagtaa tactgatcgg catgccaggg tgtgaatcct ccaccggcct
    13261 ctttgaacag ggcctgatca tgatacaatc ttactccttc acattgcatc agttcagccg
    13321 caatttttcc taatcgcctg ctcaatacca actcactgac ctgctcatct tcgagccaga
    13381 gattgaacaa ctgcagaaag gctttgccgt aggtgtttct ttcatccaat gagcgggttt
    13441 ccttgttcat actacggacc cgttctgtga tgacttcatt gagccagttc agtacctccg
    13501 gggagaatac ctgtttgagt ttgataaacc ggttttgctg gtagaaactg atctgttctt
    13561 ttgagagtgc gtagtcttcc ttcaggaagg agctaacaaa tgaggggatg gcagacatat
    13621 cgtggtttta gactacaaat ttcactgctc tttcctttgt tttctggtac aaagaatcca
    13681 aatattagta ctatattgac attaatctgc ctgatgttac agaaaatagc aatatagtat
    13741 gcggccaaag ctcgaaaaag tcagtgccgg caatggatcc tttctcagtt ttgaggtaag
    13801 gcaattgtcg tttgattttt tctggcattt ccatccggaa tatgagctaa cctatattgt
    13861 atccggtcag gggaaacgat tggtgggaga cagtgtcgaa accttcggtc cgggtgattt
    13921 tgtattgatc ggtcccggcc tgccccatgt atgggtaagt gagaagaaga ccgaccagct
    13981 tgccgtggcg ttagtggtac agttcagtgc ggcatttgta gaatcggtgc tgtcttttcc
    14041 ggagctatcg gcagcagaca gtttgttcag aaaggcacaa cagggaatct gtttccaggt
    14101 ggcaagacat tggaaaccgg aacgtaaaat gaaagtcatt gccggactac aacccggaaa
    14161 cccttttacc gttctgctgg aacttttgca ggagttgact gttaaaaagt ccaggacact
    14221 tgcttccgct cagtataagt tgttaaggtc tggaaacgac ggccgaagga tacataaggt
    14281 tttgaagttt gtgcaggatc attaccggga gccaattact gtcagccagg cggctgccat
    14341 gctgcattta tccgacagtg cattttgcaa atactttaag cgcagtatgg gtaagacgtt
    14401 ctcagatttt gtcaatgata tccggatcac ccaggccgct tcgctgctta tggaaacgga
    14461 tctctccatt gccgcactgg cggcggattg cggatttgag aacatcagtt atttcaaccg
    14521 ggtctttctg cgcaaaaagg ggatccaacc ctataagttc agggaatcga gaagagaaaa
    14581 caggaaatat aatatgccga atgactgaac aatcaaacaa atgaagtata ccattatagc
    14641 cctgttattg tttttatcgg cctgctccgt cagcaaaaat ccgttgcgaa agcaggtgaa
    14701 gttcttgcaa aagggcatca ttaaagacga taccagttat atctatgccc tgccctatga
    14761 agaaggcaag accttccgcg tgatccaggg ttattttagt cgctttaccc ataaggaaag
    14821 agccgccctt gatttcaata tgaaaagggg tacgaagatc tgtgccgccc gggagggagt
    14881 ggtggtacgg gtgaaagaag atgggaagaa agggggactc gataaaaaat accggtcaga
    14941 tggcaacaat atcgtcatcc agcatcccga cggatcaaga gccggttact ggcacctgca
    15001 atacaacggg gcattggtga atgtagggga tacagtgaag aagggacagg taattgcttt
    15061 aagcggcaaa acgggatatg cgttcaatcc gcacctgcat ttcttagtct ggcgttcatc
    15121 agggggcaat tggcagcagg tgccgacacg gtttatgacg tcgaaggggg tgaagtacct
    15181 gagggggtgg aggcggtata ctgttggtga gaacaccaac agtaggcggt gagatacagt
    15241 tggtgaaaac accaacagta ggcggtgaat acagttggtg aaaacaccaa ctgaatgcgg
    15301 tgagtacaca atctgtacga gctgatgaaa tcaacaggta caactaaagt tagcgagata
    15361 gtagttggtg ttttctccaa ctactaatta gatagatcat aatgcctgga gcgctgcaag
    15421 aatttctgct gccgtatatc ctccattttt aagataattt gaaatttcat cttttgtcag
    15481 attctccatg ttcagcaccc tggaggttgt aaccgcgtca actccggcag atttcgcccc
    15541 tttacaaaaa ccggtgaata tccaggctgc tcgttcacgt ataatcagca ggtttttcag
    15601 tactacatct tctttaaaac cggattggag caacgctttt acattatttt caatattctg
    15661 ccagtttgca ggactgccgg tgaccatgta cttgttaata aaatagaaga tgtcagaaac
    15721 agaggcaccg ccatctttaa gtgttttaat gtaaaaatgc gggtaatcga catagcccct
    15781 ttcaactggt aaaccatacg agatctttaa tgtatcactg gatgtgaaaa tccaggtagt
    15841 ggaattaccg tatgttctta tttcctttaa agcggtataa aatatccgga aaacaactgg
    15901 aaaacgtatt tccgggtatt gtgttttaac cccatcagct aattccccgg ttttgaaccc
    15961 gctatgatac atcacaatta gattctctgt catttcctgc tgaaactgag ggccatattt
    16021 tggaatatat gttttaagga tttgtccggc agaaaagtgt gctttcttca gactgtcaac
    16081 ctgtttgatt ttagctggta aaccagctgg cattttgagt tttggctgat tctgtgcagg
    16141 tgatttcacg gttgtaacct gggcgtggct gtactgaaaa aagaatgaaa aagagaagag
    16201 tagtacaaac agggattgtt tcatatttca gcgtgattta tatgtttata ggactttacc
    16261 atttaatact tttcaaaatt tctttgatca ccaacggata ccttacctgg tcttctctca
    16321 gcaagcccac cccgatatag atgatttctt tttcatccgc ataccaatag tgctgttgca
    16381 gcatcagttc catgaacgta tgattggtgg ttttaaaggt tttccctccg atgaggatat
    16441 cctgtagcgg gttttgctgc aggttgatgg ccggattcat ccgctgactg agcaggatca
    16501 gctggtcaag tgtaagctta ccggtattcg gtgtccggat cacctgcatg atctctgaat
    16561 tgtttgtact gagtaatgcc agccggttat tggtttgagc cttactctgg aagatggaat
    16621 tattgcggtc cgatttatag ctccattccc agggataccg gatcctgact ttcggcggtt
    16681 gtatccgtaa gctcttccaa cggggataat ccactggtga aaaatagtct tcgatcaccg
    16741 gcaccaacca ttgtaaagac tgttttaccg tggtaaaact gtctttacgc actgtgccca
    16801 ccaggaaact ggcttcatac gaagtggcaa tcgtggtacc gcgggtatca ttgttatgcc
    16861 aggtaaccgg gacgattgac gcgatcacct gtttgttttt gtacaggtgg tgacggaaca
    16921 gtccttccga aaaaccttcg ggaagggtgt ccatcgtgac accttgcggc agacgtactg
    16981 attgcgcagt agtatagcat gaaagtccta gtaagaacaa aagaccaatt attctcatgg
    17041 gaaacggttg aatggttaat ggtaatactt actgttttac aatttccacc ctacggttca
    17101 atgcctttcc ttcttcggta ccattggtgg ctacagggca aagtgatccc actccatcgg
    17161 accccagtct ggtagcggca attttacctt ttgtcaccag gtaatccttt actgctttgg
    17221 cccgggcttt agacaaagtg atattactgg caaagagtcc cgtattatcg gtatgtccca
    17281 ctacgtagat tttgataccg ggattagcta tgaggtaatc aattaccatt tgtaaggcag
    17341 gagcagattc aggtttgatc acggacttac ctacgtcaaa taatatcccg tataatgcga
    17401 tctttccatc acggtcaata ccttcactca tatttacttc tatatcctgt ttcatttctt
    17461 cggtctcaac gatgaaaaga tcgaaacctt gatcatcaga atcttctgca ttatcaatct
    17521 taaaccagac tgttttccca ttctggttca ttttaaaaaa atcctttctg agggatagtg
    17581 atgagccctt tgcttttacg actgcattgt gatagttgaa acggatctgt tctccactga
    17641 atttccggga ttctcccttg attttggaat acctgagacg ataaaaacga ccttccactt
    17701 ctttataaat ccgcttgtct ttcaaatcaa tgtaggaaaa cttatacgtg ctgaactcac
    17761 tcaggtcaca attttccaga agaaaacccg gcatacgggt catgtatttc ggctcaatcg
    17821 tatcacagcc tcttttgtcc tgagccgaag agaggagact gatgctgact aaaatcaata
    17881 aggccaaacg tttcatatac tatggtttgt ttttgaaaat gctgttataa tagttgtatt
    17941 tctcccgcaa acatgctttg tcccatggcc gaagcgctgc tgctgacatt cagtccactg
    18001 ttcactccaa acgtcacatc cccactggca ctgagtcctt ccagtccgag cgtggaggat
    18061 atctccattg ccgaagcgcc aaccgataat ttatttccaa catccgttac attaaaatca
    18121 ctgtcgaaac tgatgaactg tgtggcgctg gcttccacac tcagcactcc ggcttcggc
//
LOCUS       DAOPMW010000026        17643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3105_length_17643_cov_7.725893, whole genome shotgun sequence.
ACCESSION   DAOPMW010000026 DAOPMW010000000
VERSION     DAOPMW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_17643_cov_7.725893"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLU11_02605"
     CDS             <1..679
                     /locus_tag="PLU11_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN58028.1"
                     /translation="QRAAQGGSIPYDARKLLSTSTIGGQPVAMKYLYNYLNYNTLAPA
                     SLLTKNGKWFLFRQTHLHMRFAEAANRAGKYRLSWGFLNSGIAGAYPAPTSDVTLYHN
                     SLGEPYPFNFDGRNSGSSGVPYYRSDWYRNIGIRARANLMDITVPAADSLLTIENALI
                     KETALENAFEGTRWPDLVRVALRRNDPSFLADKIYNKLLKDGTGDAAAVRTKLMNPSN
                     WYLPFNW"
     gene            813..2183
                     /locus_tag="PLU11_02610"
     CDS             813..2183
                     /locus_tag="PLU11_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN58029.1"
                     /translation="MNKRFLSLILLTTGIALQAQKQNDVTTPLHAMQPDYPVPYVIPG
                     KTDVKKVLDRIYNYLDTVTPPVMINKKTGAVLSDPAQLDTNSTVKSGDFRLTSYEWGV
                     TYAAMLRAAETTGDKRYATYVKDRFDFLSKWVPAVKAKFPVDYIRSQRFLHQPIDPHA
                     LDDAGAVCAAMIKAQRAGLNEDLRPQIDVFINYIVNKEYRLKDGTLARNRPLKNTIWL
                     DDMFMGVPAMAQMGKLTGQKKYFDEVVKQIRQFSGRMFNKELGIYMHGWVESMNPHPE
                     FHWGRANGWALLTLTEVLDVLPENHPGFKFVLDQYRAHVKGLAKYQDGTGFWHQLLDK
                     SDSYLETSATAIYTYCIAHGVNKGWLDVKAYAPMSLMGWSAVASKVNDKGQVEGTCVG
                     TGMAFDPAFYYHRPVNVFAAHGYGPALFAGAEITALLDQFSFRINDSAIMLDETKSGS
                     NVPLRW"
     gene            2220..4205
                     /locus_tag="PLU11_02615"
     CDS             2220..4205
                     /locus_tag="PLU11_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPN58030.1"
                     /translation="MKQLKFVKLLVVLLMLSLSGVAQTGNQTNIRKVNITVNKPTTAI
                     SPNMWGIFFEDINFGADGGLYAELIKNGSFEFPMPMMGWRDPDTEAQGVMLIVNQAAH
                     GVNPRYAQLKLKGTGDFVLSNDGFRGIGIQKDETYLFSIMAKQLSGQGNIRLEMINAK
                     GTVIGKAEPVAVNGDWKKYNLSFTASDTAQKGKLRVIFNGNGELHMDRLSLFPQHTWK
                     NRPNGLRNDLVQKLADLKPGFLRFPGGCIVEGRDLTNRYQWKTTVGAPEDRKLIMNRW
                     NVEFAHKNAPDYFQSFGLGFYEYFQLAEDLGAAPLPILNCGMACQFNSSEVVSLPELD
                     PYIQDALDLIEFANGEVSTKWGKLRADMGHPAPFNMRLLGVGNEQWDEQYVERYALFE
                     KILKQRYPEVKLIAAAGPFAEGAYFEYAWKAFQSYKPDFIDEHYYKGPDWFFKNAARY
                     DNYDRKGFKVFAGEYAGHGKDNAQAESKNSWLSAMSEAAFMTGLERNADVVQMCSYAP
                     LLAHVDAWQWRPDLIWFDNLRSMATPNYYVQQLYGTNKGTHSVPALLDGKALSGKDSL
                     YASAVIDSVTKELIIKLVNAGEKTESFEFSLAGIKALKGEMTITTLRNEELLSYNTLD
                     KPMSVQPVSTQILVKGKKITQVTQPRSFTVIRVKYVQ"
     gene            4195..6651
                     /locus_tag="PLU11_02620"
     CDS             4195..6651
                     /locus_tag="PLU11_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPN58031.1"
                     /translation="MFNKKIQLLLALFVSVNVFAQTSDPMLLWYNKPAKDWNEALPLG
                     NGRLGAMVFGRAGEELIQLNEQTLWTGGPVNPNPNAEAPKYLQPVRDELFRDSIGRAV
                     RLLRKMQGPNTEMYQPLGDLKIKQMFTGEASNLYRDLHIGKAIATSSFDAGGVHYTRE
                     LFISAPDQVIVIRLKASKAKALQFTVDVSHDLQYHKSVTPDKVLVLSGKARITNDERR
                     NPKPLVYEDSVRCDGVRYQFRVKAVTADGKVNHTDSSLLVSDATEVVLYITAATSFNG
                     IDKCPDSEGKDEVKLATEAMNKASKKNFTTLYTAHLKDYQTYFNRVQLTLNKKTVPAL
                     PINERLEAYMKGAPDFALEELFFQYGRYLLICSSRPGGFPANLQGIWNNLLRPSWRSN
                     YTTNINLQMNYWPALPANMTEMNTPLIDHIKRMAKNGTATAKNYYNMRGWAVHHNSDM
                     WAQTNPVGEGGGDPKWANWSLGSPWMSQHLYEQYRFTADKKYLKEVAYPLMKGAAEFC
                     LDWLVERNGVLVTAPSTSPENVYLHPNGFKGTVTIASAMDMEIIWDLFTNMIEASEIL
                     GIDKEFAQLLKEKRAKLNPLKIGKKGNLVEWYDDWEDEEPTHRHVSHAFALHPGRQIS
                     PLLDTNYSNAVRKTLELRGDDGTGWSIAWKINFWARLLDGDHAYKLYRNLLHITKGYT
                     EEKYDGGGGVYPNLFDAHPPFQIDGNFGATAGVTEMLVQSHLDHIQLLPALPAAWKEG
                     QVKGLLARGNFVVNIAWNNGQLSDASITSNKGGICKIRTARPLTVQGLSIKSEGNEQT
                     GYVLSFPTKPGQRYILNAVK"
     gene            6648..7988
                     /locus_tag="PLU11_02625"
     CDS             6648..7988
                     /locus_tag="PLU11_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HPN58032.1"
                     /translation="MRQRDFLFGLFFFALQAGAQTVPMRFDFGHGKLMPGYTKVSTDT
                     RYSDETGYGFDKYSTDLQSEDGQTKNALLTDYISSSKPFYFSVKLPEGNYDVKVILGD
                     PLGTSAATIRAECRRLMITNAATGKGKFTTLNFTVHVKDSLIRNEKNEVVSKVRLKER
                     EINYLHWDNKLTLEFNGGAPKICAVEITPNKTATTVFLAGNSTVVDQDKEPWAAWGQM
                     IPSFFQAKKIAIANYAESGEALNSFRSAKRLEKVLSLMKKGDYLFIEFGHNDMKQKGE
                     GVGAFTTYKKELLRYIADVKAKGGIPVLVTSMHRRSFDSTGHIINTLGDYPEAVRQAA
                     KETNTALIDLNAMSKTLYEAWGPVESIKAFVHYPANTFPGQDKKLEDNTHFNTYGAWQ
                     IAKCIVKGIKEAKLGIAPSLRKDRVTFDPAHPDPLEKWNWPMGAYVTGMKPDGN"
     gene            8467..9813
                     /locus_tag="PLU11_02630"
     CDS             8467..9813
                     /locus_tag="PLU11_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58033.1"
                     /translation="MHKLIFFLALLSTLNASAQIGRRFPSERKEIIDPVTGHKLIFLT
                     STPNGDSKIYQTHNQWTSDGQWLIFRSNRARNEAIAVNEKTGDIVQVTEGGYTGMLNV
                     GRLSMKLYFLRYKNDRPNDTARRRPTGPLQVIEVNLAAVLDDSKAGKMKSADTYQRVC
                     GTIPEELDGGGDLALDGDEQWVYFKTGKKSAAKYLPDSVKPAATFGPRNMGAGPNGIA
                     AMNIKTGETKYVVAVPFQIGHIQTNPWMPGEIVFCWETGGKSPQRTWTVMADGKGLRP
                     VYPESEYEWVTHEAVISKDEVAIAIMGHRKIGGVDTSGTAVGGANPGQDPAWGPSGTR
                     EKPTGLAIFNLRTREVLIAGQTKKGSGLWHVSGSADGRFAVGDDFSRSIYLIDRRSGE
                     MILLSTGHKTTASDHPHPTMSPDGTKIQIQSAMLSVDGKSMNICVIPVPEEWVRRK"
     gene            complement(10122..10259)
                     /locus_tag="PLU11_02635"
     CDS             complement(10122..10259)
                     /locus_tag="PLU11_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58034.1"
                     /translation="MKFYFVACGDRVKCLGHAMRGLRAAHSLLGDGERGDEFESGFYP
                     G"
     gene            10394..10942
                     /locus_tag="PLU11_02640"
     CDS             10394..10942
                     /locus_tag="PLU11_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58035.1"
                     /translation="MKAKDLFLIILKVFGIYLIKEVLTAIPPVLYSILRMNEISLGWG
                     FTELILSFLTLCIYTGICYLLLFQTRWIVSKLQLTSGLSEEPLVMNLHRSSVYTIAII
                     VAGIVVLAFSVPQLVRSIYQWSQFMDQRRAMLSTDYFNYEGMIVSFTEVISGLLFLGN
                     QRTIVNFIESRRRKTTAEGSGE"
     gene            11764..11937
                     /locus_tag="PLU11_02645"
     CDS             11764..11937
                     /locus_tag="PLU11_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58036.1"
                     /translation="MKNKKNIVTSTSSNGAAVFKKMMEDKKAIREHIQKGGKIADIKE
                     KFNFVKPLSITGK"
     gene            11906..12397
                     /locus_tag="PLU11_02650"
     CDS             11906..12397
                     /locus_tag="PLU11_02650"
                     /inference="COORDINATES: protein motif:HMM:NF039676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6169 family protein"
                     /protein_id="HPN58037.1"
                     /translation="MLSPYPLQVNDEYSFEFITETGIRYKIYFLDYSYLFNDYTHITC
                     PVYSFNIDAIEGNPDLSPGDDRVGATVSVVMNLFFSQVDNVGVYVCDTLDDRQQARKR
                     KFDSWFYAFNDGSLIKEDGLAIVEGTEIYNALLLHKNNSQLTEIILAYKELNERAGDK
                     DIS"
     gene            12434..13015
                     /locus_tag="PLU11_02655"
     CDS             12434..13015
                     /locus_tag="PLU11_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58038.1"
                     /translation="MRIIIFLLLLTLSTQALAQDSTKRYTFKEVGWTITIPAGFMEVD
                     SVENRKNMERGKEVMEESNEVIADISEVKTLFSTTKNTFQYFTATLTRFNLQQDGDYV
                     KANNDLKGITYNTLKGQMPDAEVDSSTKKITIDGLEFDKFSISIKMKDLSLTLVLISK
                     LYKGYDFGISYLYIDEETREQLEACIRSSKFRK"
     gene            13336..14685
                     /locus_tag="PLU11_02660"
     CDS             13336..14685
                     /locus_tag="PLU11_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58039.1"
                     /translation="MERKRLIRITNNRIFLTPEMSLPMSQTSLPGEYISFRTHDDIFF
                     EIEGDYDKDKQEITVKVVDYHPSDTKRFDVQLAKAPVRFIHFAPLSWQRIERYLASYT
                     MKALLKKNIVFEEAGYVRVNEQTGSSFRAAIDRGRQADRGAMISVSDQEPGYETGKQH
                     QPVIETITEEARIYFKDAVFELGFLSFSYKSKNLKGEFNLKIENHYLLPEFNAIRNYF
                     PKALGGKKQFSIKVRFTVTDNKVTDIVTTSSDIDRINEDLLDSVKRMRIAGLTKTPLQ
                     ITVDKSLFTSSDIFDSFKEEEKDGNVFNQSEQDILNMLIEDGNIRNARQLQYLSGSKH
                     SANHKLRFTLKPNFGFLFFIEGETKNHFCWELLNSHATYLWSFDKMDSDIKWQYKQIE
                     DIINIIREMGRENYKQHYRSNPAGSDLIFSSIDHRDAGSALKDGFVEWQYRLRERLV"
     gene            14833..15156
                     /locus_tag="PLU11_02665"
     CDS             14833..15156
                     /locus_tag="PLU11_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN58040.1"
                     /translation="MAKKVKLNTLSDMKDEFVGKPETPKSVRFEYELSMEVLGYMIKK
                     ARQERNLTQEQLGELVGVQKAQISKLESSANSATIGTILKVFKGLKAEIHFNIKMGDK
                     KLQLS"
     gene            15185..15712
                     /locus_tag="PLU11_02670"
     CDS             15185..15712
                     /locus_tag="PLU11_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58041.1"
                     /translation="MPKKKNYHIDIKIDKLTNSIQNTISGDSFVTDVHLARKEDIRNI
                     TKKNGWLFSWQSEFRLKDRQVYKLTIRDNPGIIQGLASISDFNDHYYLHLVESAPFNL
                     GRKKLYEGVPGNLFAFACKLSWDAGYLGFVAFNSKTRLISHYEKTLGAIHVGGHKMII
                     FSDQALKLINKYFPI"
     gene            15718..15933
                     /locus_tag="PLU11_02675"
     CDS             15718..15933
                     /locus_tag="PLU11_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58042.1"
                     /translation="MGHIKEPDGVDLVIGPSTITEKEKGLISWIIQYYKRTGKMPSKR
                     LIASRNELPKPVSRKSAITRVRAKAIK"
     gene            16009..16587
                     /locus_tag="PLU11_02680"
     CDS             16009..16587
                     /locus_tag="PLU11_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58043.1"
                     /translation="MTPKQTERLRKKITAIRRTLAAEKRKFGCYDDGRGLRYLPTKYY
                     IQLADYKGGLTYTRWFQKNFPDDCGFPDFLFEWTILLFKTGKRKDAEEKAFQTFCSNT
                     YLFDHFFGKPIVPVEKWEGSNLEAPEFSTSIGYTSGQADLADFSTWLESWLSSPDFYA
                     RSTKFIEIQKRLLTEKDPETRRYLVLAATQLS"
BASE COUNT         5077 a         4048 c         4141 g         4377 t
ORIGIN      
        1 acaacgggct gcacagggtg gttctattcc ttacgatgcc cgtaaattat taagcacttc
       61 caccatcggc ggacaacccg ttgccatgaa gtatctctat aattatctta actataatac
      121 actggcaccc gccagcttgc tgaccaaaaa tggcaagtgg tttcttttcc gtcagactca
      181 cctgcacatg cgctttgcag aagcggcaaa cagggccggt aaataccgtc tttcctgggg
      241 tttcctgaac agcggtatag caggtgcata cccggctccg acttcagatg ttaccttgta
      301 ccataattca ttgggggaac cctatccctt caatttcgac ggacgtaaca gtggtagttc
      361 cggtgtgcct tattatcgtt ctgactggta tcgcaatatc ggcatacgtg cccgcgccaa
      421 cctcatggat attactgttc ctgctgcaga cagcctgctg accatcgaga atgccttgat
      481 taaggaaacc gccctggaaa atgcgttcga aggaacaaga tggcccgacc tggttcgtgt
      541 agccctccgc cgcaatgatc cttctttcct ggccgataaa atttataata aactgctgaa
      601 agatggcaca ggagatgccg cagcagtccg gaccaaatta atgaaccctt caaactggta
      661 tttgcctttt aactggtaaa gaaccagtac tacgttagtt cttttagcaa gcagtcgata
      721 gaaaaaggca gggaatcaag tacctgtctt tttcatttta aagaagcccc gtaacttcat
      781 agcttacata ttgtaagtaa tactatttca tcatgaacaa acgatttctt tcgctcattc
      841 tgctgacaac gggtattgca ctccaggccc agaagcaaaa cgatgtaacc accccgcttc
      901 atgccatgca gccggattat cccgtgccat atgtcattcc gggaaaaacc gatgtgaaga
      961 aagtactgga ccgcatttac aactacctcg ataccgtgac gccaccggtc atgatcaata
     1021 aaaaaaccgg tgcggtatta agcgatcctg ctcaactcga tacgaacagc acggtaaaat
     1081 ccggcgattt ccgattgacc agctatgagt ggggcgttac ctatgccgcc atgctgcgtg
     1141 ctgctgaaac cactggtgat aagcgttatg ccacttatgt gaaagatcgt tttgatttcc
     1201 tcagcaaatg ggtgccggcg gtcaaagcaa aatttcccgt cgactatatc cgcagccagc
     1261 gcttcctgca tcagccgatc gatccgcatg cactggatga tgccggagcc gtttgcgcgg
     1321 ccatgatcaa ggcacaaagg gccggactca atgaagatct gcgtccccag attgatgtat
     1381 tcattaacta tatcgtcaat aaagaatacc gcctcaaaga cggcaccctc gcccgaaacc
     1441 gtccgttgaa aaataccatc tggctcgatg atatgtttat gggtgtaccg gccatggccc
     1501 agatgggtaa actgaccggt cagaaaaaat acttcgatga agtggtaaaa caaatccgcc
     1561 agttctccgg ccgcatgttc aataaagaac tcggcatcta tatgcatggc tgggtggaaa
     1621 gcatgaaccc ccatcctgaa tttcactggg gacgcgccaa tggatgggcc ttactcacgc
     1681 tgacagaagt attggatgta ttacccgaaa atcatcccgg ctttaagttc gtgcttgacc
     1741 agtaccgcgc ccatgtaaaa ggactggcca aataccagga cggcaccggc ttctggcatc
     1801 agttactgga taaaagcgac agttaccttg aaacatcagc caccgctatt tatacctatt
     1861 gcatcgctca tggggtgaat aagggatggc tcgatgtaaa agcctatgcg ccgatgagtc
     1921 tcatgggctg gagtgccgtg gcttccaaag taaatgataa aggacaggtg gaaggcactt
     1981 gtgtaggcac cggaatggct tttgatcccg ctttctatta tcatcgtccg gtgaatgtat
     2041 tcgccgccca tgggtatggt cctgccttat tcgctggtgc ggaaatcact gccctgctcg
     2101 atcagttcag cttccgcatc aatgacagtg cgattatgct ggatgaaaca aagtccggca
     2161 gcaatgtacc cctgcgctgg taagaaaata aatattgaat tccgaataaa gagtcagata
     2221 tgaaacagtt gaaatttgtg aagttgcttg ttgtgttatt gatgttatca ttgtccggtg
     2281 tggcacaaac cggaaatcag accaacatac gtaaagtaaa tattaccgtc aataaaccca
     2341 ctacggccat ctctccgaat atgtggggga tcttttttga agatatcaat ttcggtgccg
     2401 atgggggctt gtatgccgaa ctcatcaaga acggctcctt cgaattcccc atgccgatga
     2461 tgggctggcg cgatccggat actgaagcac agggtgtgat gctgatcgtc aaccaggccg
     2521 ctcatggcgt gaatccccgc tatgctcaat tgaaattaaa aggcacgggt gattttgtgt
     2581 tgagtaatga tggctttcgc ggcatcggga ttcagaaaga tgaaacctat ctcttcagca
     2641 ttatggccaa acagttgagt ggccaaggca atatccggct ggaaatgatc aatgccaaag
     2701 gcacggtgat aggtaaagca gaaccggtag ccgttaatgg tgactggaaa aaatacaatc
     2761 tctctttcac cgccagtgat accgcgcaga aaggaaagct gcgggtgatc tttaatggca
     2821 atggcgaact gcatatggac cggctctctt tatttcccca gcatacctgg aagaaccgtc
     2881 ccaatggctt acgcaatgac ctggtacaaa aactcgccga tctcaaacct ggcttccttc
     2941 gtttccccgg tggttgtatc gtggaaggac gagacctgac gaaccgctac caatggaaaa
     3001 caacggtagg ggcgcctgaa gaccgtaaac tgatcatgaa ccgctggaat gtggaattcg
     3061 cccataagaa tgccccggat tattttcaaa gtttcgggct gggcttctac gaatactttc
     3121 aactggctga agacctcggt gccgctccat tgcccatcct gaactgcggg atggcctgtc
     3181 agtttaacag cagcgaagtg gtcagtctgc ccgagctcga tccctatatc caggatgccc
     3241 tcgatctcat tgagtttgct aacggggaag tatccacgaa atggggcaaa ctccgggcgg
     3301 atatgggaca tcccgcacct tttaatatgc gcctccttgg agtgggtaat gaacaatggg
     3361 atgaacaata cgtggagcgc tatgctttgt tcgagaagat actaaaacaa cgctatcccg
     3421 aagtaaaact gatcgccgcg gccggaccct ttgcggaagg tgcttatttt gagtatgcct
     3481 ggaaagcctt tcaatcctac aagcccgatt ttatcgatga acattattat aaaggccccg
     3541 attggttttt taaaaatgcc gcccgttacg ataattacga ccgcaaaggt tttaaagtct
     3601 ttgccggtga atatgcagga catggaaaag acaatgcaca agctgaatcc aagaactcct
     3661 ggttgagtgc gatgtccgaa gccgctttca tgactggtct tgagcgcaat gccgatgtgg
     3721 tgcagatgtg ttcgtatgct cccttgctgg cccatgtgga tgcctggcaa tggcggcctg
     3781 acctcatctg gtttgacaac cttcgcagta tggccacacc caattattat gtacagcagt
     3841 tatatggcac caataaaggc acccattcag ttcctgcctt gctcgatggg aaagccctga
     3901 gtggcaagga tagtttatat gccagtgccg tgattgattc tgttacaaaa gaactcatca
     3961 tcaaactggt gaatgccggg gagaaaaccg agtctttcga attcagtctg gctggtatta
     4021 aagcgttgaa aggagagatg accataacca ccctgcgcaa cgaagaactg ctgagttata
     4081 ataccctgga taaaccaatg tcggttcaac cggtcagcac acagatattg gtgaagggaa
     4141 agaaaattac acaggttaca caaccccgct cattcacagt aatacgggtg aaatatgttc
     4201 aataaaaaga tacagctcct gcttgccttg tttgtgtcgg tgaatgtctt tgcccagaca
     4261 tccgatccga tgctgctctg gtataataaa cccgccaaag actggaatga agcccttccc
     4321 ctgggcaatg gacgattggg ggccatggta tttggaaggg cgggagagga gttgatccaa
     4381 ctgaatgaac aaaccttatg gaccggtggt ccggtgaacc ccaatcctaa tgcggaggca
     4441 ccgaagtatt tgcagccggt aagggatgaa ctattccgcg acagtatcgg ccgcgccgtc
     4501 cgtcttttac ggaaaatgca gggtcccaat acggaaatgt atcagcccct gggcgatctg
     4561 aagattaaac agatgtttac aggagaagcc agcaatctct accgcgatct tcatattggc
     4621 aaagccatcg ctacttcttc ctttgatgcc ggtggggtac actatacccg cgaactattt
     4681 atctccgcac cggaccaggt aatcgtcatc cgcttaaaag ccagtaaggc caaagccctg
     4741 cagttcacgg tggatgtatc gcatgatctg caataccata aatccgtaac acccgacaag
     4801 gtattagtct taagcggcaa ggcccgcatc acgaatgatg aacgccggaa tcccaaaccc
     4861 ctggtctatg aagattccgt ccgctgtgat ggggtgcgtt atcagtttcg cgtgaaagcg
     4921 gtaaccgccg atgggaaagt aaatcataca gatagttcgc tactggtcag tgatgctacc
     4981 gaagtcgtac tgtatataac cgccgctacc agtttcaatg gcattgataa atgtccggat
     5041 agtgagggaa aagatgaagt aaaacttgcc acggaggcga tgaacaaagc cagtaagaaa
     5101 aatttcacca ccctctatac cgctcacctc aaagattacc aaacctattt taaccgggta
     5161 caactcacgc tgaataaaaa aacagtaccc gcgctgccca tcaatgagcg gctcgaagcc
     5221 tatatgaagg gcgctcctga ttttgcattg gaggaattgt ttttccagta tggccgttac
     5281 ctgctcatct gttcttcaag acccggtggt tttcctgcta acctgcaggg tatctggaat
     5341 aatttattac gcccctcctg gagaagtaat tataccacca atatcaatct ccagatgaat
     5401 tactggccgg ccttaccggc gaatatgacg gagatgaata ctccgcttat tgaccatatc
     5461 aaacggatgg ccaaaaacgg aaccgccacc gcaaagaatt attacaatat gcgtggctgg
     5521 gcggtacacc ataattccga tatgtgggca cagaccaatc ccgttggcga aggaggcggt
     5581 gatcccaaat gggccaactg gagcctgggt agtccctgga tgagtcagca cctctatgaa
     5641 cagtatcggt ttacagccga taaaaaatat ttaaaagaag tggcttaccc gctgatgaaa
     5701 ggcgcagcgg agttttgtct cgactggctg gtagaacgca atggtgtatt ggtcactgcc
     5761 ccctccactt ctcctgaaaa tgtctacctg catcccaatg gcttcaaagg caccgtgacc
     5821 attgcatcgg cgatggacat ggagatcatc tgggatctgt ttacgaatat gatcgaagcg
     5881 tcggagatac tgggtattga taaggagttt gcacaattac tgaaagagaa aagggcaaag
     5941 ctcaatcctt taaagatcgg gaagaaaggt aatctcgtgg agtggtacga tgactgggaa
     6001 gatgaagagc ccacgcaccg gcacgtatca cacgcgtttg ccttgcaccc gggtagacag
     6061 atatcgccct tgctggatac gaattactcc aatgcagtca ggaaaacact ggagttaaga
     6121 ggcgatgatg gtaccggctg gagtattgcc tggaaaatta atttctgggc ccgcttgctc
     6181 gatggggatc atgcctataa gttgtatcgc aatctgcttc acatcaccaa aggatacacg
     6241 gaggaaaaat atgatggggg tggtggtgtc tatcctaatt tatttgatgc acatccgcct
     6301 tttcagatcg atggcaactt cggcgctaca gcaggggtga ccgaaatgtt agtacagagt
     6361 catttggacc atatccagtt attacccgct ttaccggcag cctggaaaga gggacaggta
     6421 aaaggcttac tcgcccgggg caacttcgta gtgaatatcg cctggaataa tggacaactg
     6481 tccgatgcca gcatcacgtc caataaagga gggatctgta aaatcagaac ggccagaccc
     6541 ttaaccgtac agggattatc tataaaatcc gaagggaatg agcagaccgg ctatgtactc
     6601 agtttcccga caaaacccgg tcagcgttat attttaaatg cagtgaaatg agacaacgag
     6661 attttctctt cggtttattc ttcttcgctt tacaggctgg tgcacaaacc gtcccgatgc
     6721 gtttcgattt tggccatggt aaattaatgc ccggttatac caaggtaagc accgatacca
     6781 ggtacagtga cgaaaccggc tatggatttg ataaatattc caccgatctg cagtctgaag
     6841 acggccaaac aaagaacgcc ctgctcactg attatatcag cagctccaaa cccttctatt
     6901 tctcagtaaa actccccgaa ggcaattatg atgtaaaagt tattttgggt gatcccttag
     6961 gtacgtctgc tgccaccatc cgtgccgaat gccgcaggct gatgattact aatgcggcaa
     7021 ccggtaaagg gaaattcact accctcaatt ttacggtgca tgtcaaagac agtctgatcc
     7081 gcaatgaaaa gaacgaagtg gtcagcaaag tgcggttaaa ggaaagagag attaattacc
     7141 tgcactggga taataaactc acactcgaat tcaacggtgg tgctccaaag atctgtgccg
     7201 ttgagattac ccccaacaaa accgccacta ctgtttttct cgccggtaat tccaccgtag
     7261 tcgaccagga caaagagccc tgggcggcct ggggacaaat gatcccttca ttttttcagg
     7321 caaagaaaat cgccatcgcc aattatgcag aatcaggaga agccctcaat tcattccgca
     7381 gtgccaaaag actggagaag gtactcagcc tgatgaagaa gggggattat ctcttcatcg
     7441 agtttggcca taatgatatg aagcaaaaag gagagggagt cggagctttc actacttaca
     7501 aaaaagaact gttgcgttat atcgctgatg taaaagcaaa aggcggtatc cccgtactcg
     7561 tcacgtccat gcatcgccgg agttttgatt cgaccggtca tatcatcaac accctcggcg
     7621 attatcctga agccgtgcgt caggccgcca aagaaaccaa taccgccctc atcgacctca
     7681 atgccatgag caaaacattg tacgaagcct ggggacccgt tgaatccatc aaggcctttg
     7741 ttcactaccc ggccaatacc tttcccggtc aggataaaaa attagaagac aatacccatt
     7801 tcaataccta cggtgcctgg cagatcgcca aatgcatcgt gaaaggaata aaggaagcaa
     7861 agctcggcat cgccccctca ttaagaaagg acagggtgac ttttgatccc gcccaccccg
     7921 atccgttgga aaagtggaac tggcccatgg gggcttatgt gacggggatg aagccagacg
     7981 ggaattagtg agtggtgagt agtgagtggt gagtggggtc ctaaagcagg aagttgattt
     8041 caggttggaa gcttttttgc gggactttta tttcgagcgc agcgagaaat cttgtatttt
     8101 tttctctctc ccccaccagc gtcgtcattc cgaacgaagt gagggacctt gtatttgcgt
     8161 gagtggtgaa tggtgagtgg tgaatggtga ataggtaaag tcctaaagtg ccttgtcatc
     8221 ccgaagcgaa gcgaacagag ggcttcttag tacgttgtca tcccgaagcg aggcgaacaa
     8281 agagcttctt agaacgttgt catcccgaac cgaagggagg gatggtgaat agagtaaaat
     8341 gaccacttta atttcgagcg aagcgagaaa tcttgtattt ctttttctct ccccaatatc
     8401 gtcgtcatcc cgacgaagga gggaccttgt atttaaacaa ctaaacgact aaacacaaaa
     8461 ctctccatgc ataaactcat cttcttctta gccctcttat ccactctcaa cgcctccgcc
     8521 caaatcggtc gccgtttccc ctccgagcgc aaagaaatca ttgatcccgt aaccggacac
     8581 aaactgatct tccttaccag cacacccaat ggcgattcca agatctacca gacccataat
     8641 caatggacct ccgatggaca atggctcatc ttccgttcca accgggcccg caatgaagct
     8701 atagccgtca atgaaaaaac aggagacatc gtacaagtca ccgagggcgg ttatacgggt
     8761 atgctcaatg taggaaggct gtcgatgaaa ctctatttcc tccggtataa gaatgatcgt
     8821 cccaatgata cggccagaag gagaccaacg ggcccgctac aggtgatcga agtaaacctc
     8881 gcggctgtac tcgatgacag caaggccggc aagatgaaat ctgctgatac ctatcagcgt
     8941 gtttgtggca ccatacccga agaattggat ggaggtggtg atctggccct cgatggcgat
     9001 gagcaatggg tgtattttaa aacaggaaag aagtccgcag cgaagtattt gccagatagt
     9061 gtaaagcctg ctgccacttt tggtccaagg aatatgggtg ctggtcccaa tgggatcgcc
     9121 gccatgaata ttaaaacagg cgagacaaaa tatgtggtgg ctgttccctt ccagatcggg
     9181 catatccaaa ctaatccctg gatgcccggt gagattgttt tctgctggga gaccggcggc
     9241 aaatcgcccc agcgcacctg gacggtgatg gccgatggta aaggcttacg tccagtatat
     9301 ccggagtccg aatacgagtg ggtcacccat gaagccgtga tctccaaaga tgaagtggcc
     9361 attgcgatta tgggtcaccg taagatcggc ggcgtagata ccagcggcac cgcagtcggc
     9421 ggcgccaatc ccggtcagga tcccgcctgg ggtccatctg gtacaagaga gaaaccaacc
     9481 ggcctggcca tcttcaatct ccgcacccgc gaagtactca ttgccggaca aaccaagaag
     9541 ggcagtggtc tctggcatgt cagcggttcc gccgatggcc gtttcgccgt aggggatgat
     9601 ttctccagaa gcatctacct catcgaccgc cgcagcggag aaatgatttt attatccacc
     9661 ggccacaaaa ccaccgcatc cgatcacccg catcctacga tgagtccgga tggcacgaag
     9721 attcagatac aatcggcgat gctttccgta gatggaaagt ctatgaatat ttgtgtgata
     9781 ccggtgccgg aggaatgggt taggagaaaa tagaaaatag aggccttcta aggaaattgc
     9841 cttaagcccg aagggctgac atgattatag cccctggtgt taaccagggg aaagatagtg
     9901 gctatgatga aactgaaaac cccgaagggg tggcattatt atagagttga cgttaagagg
     9961 tgtattagcc ccgaaggggt gtaacaataa tagcaaccag tccggaaatg tccctaaagc
    10021 cccgacctgc cgaccgaagc gtagcgtagg tgggaggggt gacacaatta taccccggta
    10081 aataacattc atttcaccag ccccagcggg gcgaaatatc attagcccgg ataaaatccg
    10141 gattcaaatt catcgccccg ttccccatcc cccaataacg aatgcgcggc ccggaggcca
    10201 cgcatagcat ggcccagaca ttttacccgg tcgccacagg cgacaaaata aaatttcaaa
    10261 gcaggatgct ttgaaccctt ccggtcatcc cgtttttttc aatatccctt tttcaaaccc
    10321 aatcccccaa tccccaattt ttccctacct taaatcccgt ttataatcgg caattaaacc
    10381 taactgctcc aacatgaaag caaaagacct tttcctcatc atcctgaaag tattcggtat
    10441 ttacctcatc aaagaggtgc tcactgccat cccacccgtt ttgtattcca tcctccggat
    10501 gaatgaaatc tccctgggtt ggggttttac tgaactgatt ttatccttcc tcaccctttg
    10561 catttatact ggtatctgct atctgttgtt gtttcaaacc cgctggatcg tatccaaact
    10621 gcaattaaca tccggcctta gtgaagaacc cctggtcatg aacctgcacc ggtcttccgt
    10681 ttataccatt gccatcattg tggctggcat tgtggtactg gccttttctg taccgcagct
    10741 cgtgcgtagt atttatcaat ggagtcagtt catggaccaa cgccgtgcca tgctgtcgac
    10801 cgactatttc aactatgaag gaatgatcgt ctcttttact gaagtgatca gcggactgct
    10861 gttcttgggg aaccagcgga cgattgtgaa ttttattgaa tccaggaggc gtaagacaac
    10921 ggcagaggga agtggtgagt agtgagtgga gagtggagag tggagagtgg agagtggaga
    10981 gtggggcctg ttaaggtaat tgtattaatg tattaagccc gtagggctga catgattata
    11041 gaaaatacga gaaggagtaa acagggcctc agaaccccga cctgctgaca gtagcaaagc
    11101 gaaggtggga ggggtgacat aattatagcc ccgggtgcca acccggggaa aagaatgtcg
    11161 cgttaatgaa actgaaagcc ccgaaggggt gacacaatta taggggtgac acaattatca
    11221 gggtgaaata tttgtagccc ggataaaatc cggattcaaa ttcatcgccc cgttccccat
    11281 cccccaataa cgaatgcgcg gcccggaggc cacgcatagc atggcccgtg cattttcaac
    11341 ggtcgccaaa ggcgacaata tagcatttca aagcaggatg ctttgaaatg ctgacggaca
    11401 tcccgttttt ttggatagtc ctttttcaaa cctaatcccc gtttttccct accacaaacc
    11461 ttgttcatca tctgtcatct ccccacccca tcggggtgca ttgtcggtag cttcattgcc
    11521 gccccaaatg ttcccttcac cccgtggggg tgtattgtga tgttcaggaa aagcagttgt
    11581 acaaggcata tgaaatgaat cctaagaacc ttcgctgtca acctttcaac cagtcagcca
    11641 tttcaacctg ctcattatca atccattgtt tttatacccg cttaattgtt cttctgtttt
    11701 tgatgttaaa tagccgtaac aggcctgtgt ttactaagga atttttgtaa atttgtagtg
    11761 ttcatgaaaa ataaaaagaa catagttact tctacaagct ccaacggtgc cgctgtcttc
    11821 aagaaaatga tggaagacaa aaaggctatt cgtgaacata tccaaaaggg gggcaaaatt
    11881 gcggacataa aggaaaagtt caattttgtt aagcccttat ccattacagg taaatgacga
    11941 atactccttc gagtttataa cggaaacagg aatcagatat aaaatctatt tcctggatta
    12001 cagttacctg tttaatgatt acacacatat tacctgcccg gtatattcct ttaacattga
    12061 tgcgattgaa ggtaatcctg acctgtcacc tggagatgac cgtgttggtg cgactgtttc
    12121 agttgtaatg aatttattct tctcccaggt agataatgtt ggggtttatg tttgtgatac
    12181 gctggatgat cggcagcaag cccggaaaag aaagtttgat agttggtttt atgcatttaa
    12241 tgatggctca ttaataaaag aagacggact agccatagta gaagggactg aaatctataa
    12301 cgccctttta ctgcataaaa ataatagtca actgactgaa ataatactag cttataagga
    12361 actgaatgaa agggcaggag ataaggatat ttcctaagaa gcctccctat caacatttcc
    12421 ctaaatttaa cccatgcgaa taatcatctt ccttctcctc ctcacactct ccacccaggc
    12481 cctggcccag gactccacaa agcggtacac attcaaagaa gtcggatgga cgatcactat
    12541 tccggctggt ttcatggagg ttgattccgt cgaaaacagg aaaaatatgg agcggggcaa
    12601 ggaggtcatg gaggaatcta atgaggtcat tgctgatata tctgaagtta aaaccctttt
    12661 cagcaccact aaaaatacct ttcagtattt cacagcaacg cttaccaggt ttaatctcca
    12721 acaagacggg gattatgtga aagcgaataa tgatctgaag ggaataacgt acaatacatt
    12781 gaagggtcag atgcctgatg cggaagtgga ttcttctaca aaaaaaatca ccattgacgg
    12841 actggaattt gacaagttca gtatttctat taaaatgaag gacttgtccc tgacgcttgt
    12901 gttaatatcc aagctataca aaggctacga tttcgggatc agttacctgt acatagatga
    12961 ggaaacacgg gagcaacttg aagcatgcat ccgcagcagt aaattccgta aatgaagcaa
    13021 ggtcatggta aatgcagttt ataagccggc acgcaggcct gtcaaccttt caactcccca
    13081 ccccatcggg gtgcattgtt ggtagcctca ttgccgcccc ggatgttccc ttcaccccgt
    13141 gggggtgtat tgtgatgttc aggacagaca gttgtaaaag ctgtatgaaa tcaatcctaa
    13201 gaagccctcc ctgtaaacct gcctcgccgg caggcaggcc cttcaacctg cctcagccgg
    13261 caggcaggcc tgtgaaccta tcaacctctc ccgtaattca gtaaattagc ctgtaaaacc
    13321 gcgaccaacc cactcatgga aagaaaacga ctcatacgaa taaccaataa ccggattttc
    13381 cttacaccgg aaatgagtct gcccatgtcc caaaccagtt tgccaggtga gtatatcagt
    13441 tttcggacac atgatgatat cttctttgaa atcgaagggg attatgacaa agacaaacag
    13501 gaaattaccg ttaaggtggt tgactaccat ccatcagata caaaacgatt cgatgtgcag
    13561 ctcgcaaaag ccccggttcg ttttattcac tttgcaccac taagctggca gcgtattgaa
    13621 cggtacctgg ctagctatac gatgaaggcc ttgctgaaaa aaaatattgt attcgaagaa
    13681 gcgggttatg tccgggtcaa tgaacaaaca ggttcatcat tccgtgctgc tattgaccgt
    13741 ggcaggcagg cagatagagg agcaatgatc tctgtttcgg atcaggagcc gggttatgag
    13801 actggcaaac agcatcagcc ggttattgaa acgatcacgg aagaagcaag aatttatttt
    13861 aaggacgcgg tgtttgaatt aggattcctt tccttttctt acaagtcaaa gaatcttaaa
    13921 ggagaattta acctgaagat tgagaaccac tatctcttgc ctgagttcaa tgcgatccgc
    13981 aattatttcc ccaaagcatt ggggggaaag aaacagtttt ccattaaagt ccggtttaca
    14041 gtgacggata acaaggttac ggatatagtt actacttcgt ccgacataga tcgaatcaac
    14101 gaagatctgc tggacagtgt aaaacgaatg cggattgccg gtcttacgaa gactcctttg
    14161 cagataactg tagataaatc actgtttact tcttcagata tatttgactc ctttaaggag
    14221 gaggaaaagg atggaaatgt attcaatcaa tcggagcagg atattttaaa tatgttaatt
    14281 gaagacggca atatccggaa tgcccgtcag ctgcaatacc tgtcaggttc aaaacattca
    14341 gccaaccata aattgcggtt taccttgaag cccaacttcg gcttcctgtt tttcatagaa
    14401 ggcgagacca aaaatcattt ctgctgggaa ttgctgaact cgcatgccac gtatctgtgg
    14461 tcattcgata agatggactc ggatataaag tggcagtaca agcagattga agatattatc
    14521 aatatcatcc gggaaatggg ccgtgaaaac tacaaacagc actacaggag taacccggcc
    14581 ggaagcgatt tgattttcag cagcatcgat caccgggacg ccggctctgc gctgaaggat
    14641 ggctttgtag agtggcagta tcggttgaga gagcggttgg tgtagtgggt ttaaaatttc
    14701 aaagcaacta tgctttgaaa acccaaatcc aagaattata caaaaaagat tgtttctaaa
    14761 tatggaaaca atttctactt ttgaattcta cgatgaagtc atataaaact tcaatccgaa
    14821 aaaataatca tcatggcaaa aaaagtgaaa ctgaataccc tttccgatat gaaagatgag
    14881 tttgtcggca agcccgaaac accaaaaagt gttcggtttg agtatgaact cagtatggag
    14941 gtattgggct acatgatcaa aaaagccagg caggaacgaa acctgacaca ggaacaactg
    15001 ggtgaattgg tcggtgtaca aaaagcacag atatcaaaac tcgaaagcag tgccaacagt
    15061 gccacaatcg gtaccatttt gaaagtgttt aaaggcttaa aagctgaaat tcatttcaat
    15121 attaaaatgg gggataaaaa gctgcagcta tcctgaaatc gggtcttttt tgtatcttta
    15181 tagggtgccc aaaaagaaaa attatcacat agatattaag attgacaagc tcacgaatag
    15241 tattcagaat actatttccg gtgatagttt tgttaccgat gttcacttgg cgagaaagga
    15301 ggatatcagg aacattacaa aaaagaatgg ctggctcttt tcctggcaat cagaatttcg
    15361 ccttaaagac cggcaggtgt acaaactcac aatacgggat aatcccggaa ttattcaagg
    15421 gctggccagt attagtgatt ttaacgatca ttattattta cacctcgtgg aaagtgcccc
    15481 atttaatttg ggcaggaaaa aactttacga aggtgtgcct gggaatcttt ttgcttttgc
    15541 ctgtaaactt tcctgggatg caggatatct gggtttcgtg gcatttaatt caaaaacaag
    15601 attaatatca cattacgaga agactttggg agccattcat gtaggtgggc ataaaatgat
    15661 aatattttca gatcaggcac taaaacttat taataaatac tttccaattt aataaacatg
    15721 ggacacatta aagaaccgga tggggttgat ttggtgattg gcccatccac cataacagag
    15781 aaagaaaagg ggctgatcag ttggattatt caatactata aacgtacggg gaaaatgccc
    15841 tcaaaaagac tcatagcatc ccgtaatgaa ttaccaaaac cggtaagcag gaaaagtgca
    15901 attacccgcg tccgtgctaa agcgatcaag taaaacagtc aatttatatt gggagcttgt
    15961 atacaccagc ttaaccggtt gtgctgacag ctaagttaac tactcctcat gactcccaaa
    16021 caaaccgaac gactccgcaa aaaaatcacg gccatccgcc gtactctggc agctgagaaa
    16081 cggaaattcg gttgttatga tgacggccgt ggactgcgat atcttcccac aaaatattat
    16141 attcaactgg ccgactataa aggcggactg acttataccc gttggtttca gaaaaacttt
    16201 ccggatgact gcggttttcc tgatttcctg tttgaatgga caatccttct ttttaaaacc
    16261 ggtaaacgca aagacgcaga agagaaagcc tttcagacat tttgcagtaa tacctacctc
    16321 tttgatcact ttttcggaaa accgattgtg ccggtggaga aatgggaagg gtccaatctc
    16381 gaagcacctg aattttccac atcaatcggc tatacatccg gtcaggctga cctggccgat
    16441 ttcagtacct ggctggaatc ctggctttca tcacctgatt tttacgcccg gagcacaaaa
    16501 tttatagaaa ttcagaagcg gctcctgaca gaaaaggacc ccgaaacccg gcgctatctt
    16561 gtacttgccg caacacagtt aagttaactg gttgaaaagc ctgccggccg gcaaaggcag
    16621 gttgacaagg aagcttccta agaaaccacc ctgtaaactt gtaaatctgt caacctttca
    16681 acttcacccc atcggggtgt attattggta gcatcaatgc cgccccgaac gctcccttca
    16741 ccctgtaggg gtgtattgtt ggtgtcaaaa ttatcgttgg tgaaataacg cccccatact
    16801 agccagatca gccgatcaca taaggggtgg tctatccggg aataaacgtt tatacacggg
    16861 tataaacgtt tattcccggc tatatttaaa aaaacgccct tagtttaagg cgaatgaaca
    16921 gggttgaaag aatataccag ttatagaaaa aatttcggtg aatgtgattt gcgttaataa
    16981 caaattttat aatacaatga catataggct tttaaataat tgcaatggaa gataatttta
    17041 gaaattctaa aatatattta gatttgcaat acgagccctt cctgtgtaca aaaccggccg
    17101 gagaatggta gtgatcagca aaagcacatt gaagaggttc tttgagaaat acccttcagc
    17161 gaccggacct gtactacagt ggtacggtag ggtgaaggaa gctgactgga aggactttgc
    17221 agatttaaaa aaaatgtttt cggctactga ctatgtgggg aacgatttgt atgtattcaa
    17281 cctgggtggg aacaaatacc ggctcattgc cagggttttc tttagcgtgc gtaccgttta
    17341 tattcgattc attggtacac acggagaata tgataaggtt aagctcgctg acctttaagg
    17401 aaaacaaaag cgagagcggc cttcttttaa tggtatcgtg ggtatagtca atccggattc
    17461 aaattatata atatgcaaaa tgttaagcgc cgtttggtgg taaagaaaaa gtcacggttg
    17521 aaaaaggcgg gagaagaaat gaaatcaagc accggagaaa agggtttaat aaaagccagg
    17581 aaagtgcaga tcaaaacaaa agaagcttac gacgccgtca tgcaggagat tgaccaactg
    17641 atg
//
LOCUS       DAOPMW010000027        17642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3108_length_17642_cov_8.169159, whole genome shotgun sequence.
ACCESSION   DAOPMW010000027 DAOPMW010000000
VERSION     DAOPMW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17642
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_17642_cov_8.169159"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            798..1793
                     /locus_tag="PLU11_02685"
     CDS             798..1793
                     /locus_tag="PLU11_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPN58044.1"
                     /translation="MKQLICLLVFVVSLAGNGLAQTTADSLGIPADTVRVADSQLLSD
                     TPVVIPTVLVKRPLLQTDSSWTLAADDLVAGPKLQWLILAHHPIYRFEFKPVDGVRRA
                     DLTDKGEVRVAKDKDLLFYSLLLLIIVFALLRRAFPKYFNDLFRLFFRTTLKERQISE
                     QLMQTPVPSVLLNGFFAVSAGFYCTFLLLHFGLNPTGEFWTLFIYCTGGLAAAYFIKF
                     TGLKISGWIFNLQAAANSYIFIVFIINKMIGILLLPFLLVLAFSTGTVYTVGMTLSWC
                     LIGGLMLYRLFLTYAVIRNQVKVSPFHFFLYFLAFEIAPLLLVYKALLVFFSENT"
     gene            1796..2623
                     /locus_tag="PLU11_02690"
     CDS             1796..2623
                     /locus_tag="PLU11_02690"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPN58045.1"
                     /translation="MHPTLADPNMSTNGVKKAGEQAKAIQRILITQPRPESEKSPYFE
                     LERKYSVKLDFHAFIRLEGIPARDFRKQKIEIPNYTGIIFTSRNAIDHFFRMCEEMKV
                     SVSQDTKYFCITEAVALYLQKFILYRKRKVFYGADGTNKSMFDVINKHKANEKFMYVC
                     SENQQDNEIVNWLKANNCEYTMAFMYRSVSSDVKEVLTSIDYDVICFFTPSGVKSLFD
                     NVPKFKQNGTVLGAFGTNTFRAIEEKGLKLEIKAPQPQTPSMVAALDQFLASLKKKK"
     gene            3121..5178
                     /locus_tag="PLU11_02695"
     CDS             3121..5178
                     /locus_tag="PLU11_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPN58046.1"
                     /translation="MKSTGNRLKDETSPYLLQHAHNPVDWYPWGPEALEKAKKENKPI
                     LVSIGYAACHWCHVMERESFENDATAEIMNTHFVNIKIDREERPDLDHIYMDAVQAMT
                     GSGGWPLNVFLTPDTKPFYGGTYFPPQKAFNRPSWTETLYGVAQAFRERRHEIDAQAE
                     NLTEHLLNANAFGIKMPEEADTLFHPEKLQECFTQYMKTADRQWGGFGKAPKFPQTFS
                     IQFLLRHHYFTGEPAALEQALLSLDKMIDGGIYDQAGGGFARYSTDAEWLAPHFEKML
                     YDNALLVSVLSEAYQLTHKPRYREVIDETLAFIEREMMHPGGGFYSALDADSEGVEGK
                     FYTWSRQELESELGEEAAAFCAYYDVSEAGNWEHTNILWIRKPMAEVAQSLKMEEAAL
                     SELLARGRKRLMEIRANRIRPLLDDKVLLGWNALMNTAYCKAYEATGNEKYRDQALRN
                     MDFLLSAFRDETGQYRHTWKNGQARYPAFLDDLAFLLQAMLQLQQVTGDLNWLERSRE
                     LVRLIIEKYQEPGTPYFFYTPDDQEDVIVRKKEVYDGALPSGNAVMAICLYHMSVIFD
                     MKEWKERSLDMYRMLGNAITRYPVSFGVWAMGVQEVVEGTNEIVLTGDNPEVLMADLL
                     GRYVPNKVVLTTGIKKLDIPLLEGRKGEGKPSIYLCRNNTCQAPVFSADSLISLINRD
                     KKQ"
     gene            5332..6636
                     /locus_tag="PLU11_02700"
     CDS             5332..6636
                     /locus_tag="PLU11_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPN58047.1"
                     /translation="MRNLYYALYATACMTLVSSCGILGGKKNKGGALPNDGQVHGVAP
                     GGKYTLPKPPGMVYVPQGTFHMGPSDEDPAYAFSARNRSVSISGFWMDATEITNNEYR
                     QFVNWVRDSVVARKMGYVKTGSDGNENVDWAKMKTMKWGDPKVMEQLSSTDIILPPDD
                     RVFGKKEIDPSKIVFHSETFDLKEAARRENAGKSRSTFIVKKDVAIYPDTLCWIRDFS
                     YSYNEPMTKRYFSHPAFGNYPVVGVNWKQATAFCEWRTHFLNAFLDSKKRTQESDFRL
                     PTEAQWEYAARGGRSQSMFPWGNYYLRNKKGCLMANFKPGRGNYPEDGGFYTVRADGY
                     WPNDYGLYCMSGNVAEWTSSIYYEGRNNFQHDMNPDVRWNAKDDDPPLMKRKIIRGGS
                     WKDVGYYLQTGTSTYEYQDSTKSYIGFRCVIDLPAAPQKGRR"
     gene            6753..7526
                     /gene="gldL"
                     /locus_tag="PLU11_02705"
     CDS             6753..7526
                     /gene="gldL"
                     /locus_tag="PLU11_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPN58048.1"
                     /translation="MAAAIPSKVSKWVDVGVSFGAALVIWGALRKITHAGDADTWLWI
                     GLTTEAIIFSIYGILYAVYPAVKDASHDEEQFSVAGTKSRAALGAMDKMLAESDISPD
                     TMKRLGDGFKQLNTTVSGLTTIGDTINATADFTNKTREVTGQLEKVKDAYTTAASSVG
                     AFNTATEGAKVFHEQIQVLTKNLSSLNTIYELELQESNNHLKALNSFYGKLSETSNAM
                     LNSAEDAKKVQDQIGHLATNLGRLNNIYGNMLTAMQGRS"
     gene            7617..9146
                     /gene="gldM"
                     /locus_tag="PLU11_02710"
     CDS             7617..9146
                     /gene="gldM"
                     /locus_tag="PLU11_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HPN58049.1"
                     /translation="MSLPKEPRQKMINIMYLVLTALLALNVSSEILNAFRTVRRSLEN
                     SNATVTQSTAIIMKSLEDKSNDPVTTERATPWFQKAQKVTATADAIFKYIDGLKNDII
                     TKAGGKPGDPTVTFKEDNLDIVTKMFVQGKEGDKLKQKLEQFQKDILGIDPEIDSAFK
                     KSLNIDLSNPPGQDGKSKAWDLAYFNMVPTVAGLTILSKFQNDIKGAENKVVSFCHQK
                     VGEVKVVFDSYAAIVGQNSNYLMPGQQLEVKAGIGAFSKTSKPTITIGGTTVPIGEEG
                     FALYKTDAGGVGAHSIPVRISFFNQTTGKDEVKEVKVEYTVGSANASIALDKMNVLYI
                     GVDNPVTIAASGGGDDKIQTSITAGGSLTRVGNGKYIARVNNITDDCKITVTVDGKVA
                     GASQFRVRTIPLAQAYVGGKPSGEPYQAGAFKAQGGVGAGIKDFPFELSYDVVSFTFT
                     CDTDDDIFSQPVQGNSFAPIRAAINQHVKADRMVTIDDIRVKGPDGRTTKAPSLVYYI
                     K"
     gene            9155..10177
                     /gene="gldN"
                     /locus_tag="PLU11_02715"
     CDS             9155..10177
                     /gene="gldN"
                     /locus_tag="PLU11_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPN58050.1"
                     /translation="MNMKFVKFGFCLVLSAFLLTPADAQRVSKKKKAENAAQTPPAGT
                     EQQQNNNTQPPSGYDPLAGLPMTYDTTSNDLTPKKSLRNDNAFDKSNLNQRTPLPYEH
                     LRWDDALYAEKVWRELDLREKMNKTFMYEAVDDNGSQMFVNLLLKAVQNGEVTAFSDD
                     RFTLPMDVPSVQQITAGRLDTFPVYDTKDISKIVGWTVSRASFDAKSVTRLRLKEEWV
                     FDRESSRMFVRILGIGVLKTEYMPGTTKERGTSALFWVYYPDLRPMMAKAEVYNPKNM
                     GQSRMTWEELFESRMFSSYIVKSTLDNSSNKMIRNYINDPILRLLEGENIKEKIFNFE
                     QDLWSY"
     assembly_gap    11143..11242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11243..12801)
                     /gene="uvrC"
                     /locus_tag="PLU11_02720"
     CDS             complement(<11243..12801)
                     /gene="uvrC"
                     /locus_tag="PLU11_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPN58051.1"
                     /translation="MTAAEFHLIASTIPVQPGIYKYFDSEEQLLYVGKAKHLRKRVSS
                     YFSKTFTTYKTHELVQRIARIEFTIVNSEQDAFLLENSLIKQYQPRFNINLKDDKTYP
                     FIVIKNEPFPRVFLTRQKKDDGSEYLGPYTSVKRVRELLEFIKQTIQLRNCNLNLTPG
                     NIEKKKFKVCLEYHLGNCKGPCEGLQTLADYEEYISQLRNLLRGNLAPVIRHFKEEMK
                     AHAAELAFEKAELVRKKIDYLENYQSRSVVAGLKSADLDVFYLLQEKEIAYVNYLMVR
                     NGSIIQTQTNKVDTPLDETPEEVLAFSVAALRSTFNSDAREIVVPIPIDFPQEDMIVT
                     VPRGGDKKKLLELAEKNARYFIDELQAKERLKLNSKEEAGKALLEQLQEDLTLSDLPV
                     HIECFDNSNFQGSYPVSAMVCFRNAQPFKKDYRHFNVKTVEGINDFATMKEAVYRRYK
                     RLSEEGQDFPQLVIIDGGKGQLSAANEAITELELEGRTTLVGLAKNEEEIFFTGDTQS
                     LKLPYNSPTLKL"
     gene            complement(12798..13388)
                     /locus_tag="PLU11_02725"
     CDS             complement(12798..13388)
                     /locus_tag="PLU11_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58052.1"
                     /translation="MKKMFLAGLPLLTLFLFSCKEKKKAESKGFISVVSLIEKQVAHV
                     DTSLYSIVQVVSKDSLHQDTTYIPREKFRETAREFLELPDLTDPEVAARFKEDKMYDT
                     LYRRVIITYTPLDVKKEEFKKIELLISQELKEDGSNKVTNIIADRIINNRDGFLQKKM
                     LWQTDRSFLVVTSTQQPGQPEITSTNRVTWNEENIP"
     gene            complement(13489..14979)
                     /locus_tag="PLU11_02730"
     CDS             complement(13489..14979)
                     /locus_tag="PLU11_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58053.1"
                     /translation="MESNFNNNDFERFLKQNADQYRMFPSENVWDGIHNTLHTRRRWH
                     GLWLALLLLTTGTVTWIMLTTTSGNKKEQIIAARKPEAKKETATLPPSELVTTPKANR
                     ISKPPIASPGNQEKNIFLATEVLESPESTADITGVDKASTVALAPVSTSLGNISASFP
                     AAPVNPARNPSAIVTRTAPRQPENLNEAPAVVVAAKPVIPSIADDLITDPAAISGMNE
                     KQTETAPELNSEDQVYPLTIESVINSYSVARKTRKAKWQLYITPTVTYRKLNENKQFI
                     NSARSNMSPTALTYSPDLNNLVTHRPDLGLQLGVSMAYPLTKKLSLLTGLQFNISKYD
                     IWAYNYPSEIATVALSNDYGGTSTVSTVTNYRIVGVNRANKANWLRNFYYSVSVPVGL
                     EYKAFKTRRAFVGVAATLQPTYVLGNRAYVISTDYKNYMEVPSLTRKWNVNAGFETFA
                     GFNVGKTAWRIGPQVRYQTMSSFKKEYPVQEHLFDYGFKLGIMLNR"
     gene            complement(15011..15562)
                     /locus_tag="PLU11_02735"
     CDS             complement(15011..15562)
                     /locus_tag="PLU11_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN58054.1"
                     /translation="MKNEASAQRELYNRYSSKMLAVCYRFAQNREDAEDMLQEGFIKV
                     FLQMHTFENRGAFEGWIRRIMVHTCINILKKNKKFNESVDIIHATGVQVREESVPAII
                     QAKQVVECIRLLPIGYRTVLNLYAVEGYSHREISNMLDIEESTSRSQYTRAKAMLEDI
                     LVRKRILHRPKQEFNWLAAVSRR"
     gene            complement(15774..16376)
                     /locus_tag="PLU11_02740"
     CDS             complement(15774..16376)
                     /locus_tag="PLU11_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPN58055.1"
                     /translation="MKLKASHTDPHNKRVHQAERLRLYRKIHRTTGMLLFVFFFIMSI
                     TGLLLGWKKNSGGYLLPDTYKGSSTNLSEWLTLDSLYLSACLYMHDSVSPASSTELER
                     IDIRKDKGVVKFVFNGQFLGLQMDGATGKLLHIDKRRSDFIEKVHDGSILDYYSGTSG
                     DFFKLVYTSVMGSALFVFTFTGFWLWYGPGAIRRSRKTQK"
     gene            complement(16428..17321)
                     /locus_tag="PLU11_02745"
     CDS             complement(16428..17321)
                     /locus_tag="PLU11_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58056.1"
                     /translation="MCYNTLDATFDTTFIKFPYKQAELRDGALTGNAGLVFRPAEGWQ
                     LNGNISTGYRMPNVDDIGKLFESVPGNVTIPNPELTPEYAWNFEAGIIRNSPGKYLFE
                     ITGFHTLLTNAIVRRPTTFNGQDSILFNNVLSRVEALQNVARATVNGIQLSAEVFFTK
                     SFSFVSHANWISGKETDDVKNEQVPLRHAPPFYGSTAFRLRTKKITAELNCQYNSQIS
                     NADLAPSEKAKTDIYAKDASGKPYAPGWYTLNLKASYQLIQQLMLTAGWENMSNQRYR
                     PYSSGIVAAGSNFILSVRASL"
BASE COUNT         4603 a         3941 c         4228 g         4770 t
ORIGIN      
        1 aaggccggta tcttcaacct ggcctgggca gtagggaaac gcaacgatgc ggagtttaac
       61 ctgcgccagt caaagatcca ctttggattt gtgaattact tttaaagctg ctctgcggct
      121 ttggggtggc cacgaattaa cacaaaaaga cacgcctgtc cagccttatg cgggagattg
      181 caacggattt ggttttgtat tccttcgtgt ctattcgtgt tttttagtgg ccaatattat
      241 ccgcgaagcg ggaaatcctt cccgtccttc ttttcttccc ggctaatttt ttgccggtaa
      301 ggcggtaaga acgggaagga agcctctgcg aggctttttt gttgccacga atgagcacaa
      361 aagagcacga aagaaataca gaaaggcagg ccacggatga cacgcctgtc ccgccttatg
      421 cgggagattg ccacggattt ggttttgtat tccttcgtgt ttattcgtgt tttttagtgg
      481 ccaatattat ccgcgaagcg ggaaatcctt cccgtccttc cttttgttca ctcccgacac
      541 ttcgggattc ccggctaaat ttttgccggt aaggcggtaa gaacgggaag gaagcggctg
      601 cgaggctttt ttgttgccac gaattaacac aaaaggacac gaaaataaac aagcactaca
      661 taggagtgac tgtttactgc caactgccga ctgccaactg ctcactgccg actggaaact
      721 tttcttttca gcaatatctc acaaacactt cttatttatc aaacagccct gctatttttg
      781 caacacaaat ttttttcttg aagcaactga tctgtttact ggttttcgtg gttagcctgg
      841 cgggtaatgg actggctcag actacagcag acagtttggg tataccagcg gatacggttc
      901 gggttgctga cagccagctg ctgagcgaca cccctgtggt tattccaacg gtattggtaa
      961 aacgcccgct tttacagact gacagcagct ggacactggc agctgatgat ctggtggcag
     1021 gcccaaaact acaatggctg atcctggccc atcatcccat ttaccggttt gaatttaaac
     1081 cggtggatgg agtccggaga gccgacctta ccgataaggg ggaggtgcgg gtggcaaaag
     1141 acaaggacct gctgttttac agtttactgt tactgataat cgtctttgcc ttgcttcgcc
     1201 gggcattccc caaatatttc aatgaccttt tccggctatt tttcagaacc acgttgaagg
     1261 aaagacagat cagcgaacag ctgatgcaaa caccagtgcc ttcggtttta ctcaatggct
     1321 tttttgccgt cagtgccggc ttctactgta ctttcctgct gctgcatttc ggactcaatc
     1381 caaccggtga attctggacc ttgtttatct attgcacagg ggggctggcc gctgcgtatt
     1441 ttattaagtt caccggattg aaaatatccg gctggatttt caatctgcag gctgccgcca
     1501 attcctatat ttttattgtc tttattatca acaagatgat cggaatcctg ttgctaccct
     1561 ttttgctggt actcgcattt tcaaccggga cggtatatac ggtgggcatg acattatcct
     1621 ggtgcctgat cggcggatta atgctttatc gtcttttcct gacttatgcg gtcattcgta
     1681 atcaggtcaa agtcagtcca tttcatttct ttttatactt tctggccttt gagatagccc
     1741 ccctattatt ggtttacaag gccttgttag tctttttcag tgaaaacact taactttgca
     1801 cccaacttta gccgatccga acatgagtac aaatggggtt aaaaaggccg gggaacaggc
     1861 caaagcaata cagagaatcc ttatcaccca gccgaggccg gaaagcgaga aatcgcctta
     1921 ttttgagctg gagcggaaat acagtgtcaa actggatttt cacgctttca tcaggctgga
     1981 aggaattcct gccagggatt ttcgcaaaca aaagattgag atccccaact ataccggtat
     2041 cattttcacc agccgtaatg ccattgatca tttcttccgg atgtgtgagg aaatgaaagt
     2101 cagtgtttcc caggacacca agtatttctg tatcacagaa gcggtggccc tttatctcca
     2161 gaagtttatt ctttaccgca aacgcaaagt tttttacgga gccgatggca ccaataaaag
     2221 catgtttgat gtgatcaata agcataaggc gaatgagaag ttcatgtatg tgtgctccga
     2281 aaaccagcag gataatgaga tcgtaaactg gttgaaagca aataactgcg aatacaccat
     2341 ggcctttatg taccggagtg tgagcagtga tgtaaaggaa gtgctgacca gtattgatta
     2401 tgatgtcatc tgtttcttta cccccagcgg ggtgaagagc ctttttgata atgttcccaa
     2461 atttaaacag aacggtactg tactcggagc ctttggtacc aataccttcc gggcgattga
     2521 agaaaaaggg ctgaaactcg aaatcaaagc cccgcagcca caaactccca gtatggtggc
     2581 ggccctggat cagtttctgg cttcactgaa gaagaaaaaa taagcaacga ataaattaaa
     2641 aaaggctgta cttgaaaaag tgcagccttt ttttgcctgc ggcaaaaagg ggtggccacg
     2701 aattgacaca aagtggcacg aaataataca gaaatacacg gccacggatc acacagatta
     2761 acacggattg tatttgcgaa tggttaaagg accctactga ttaagaaagg ttaatcagtg
     2821 ctgatccgtg tgatccgtgg ccttgtattt ttcgtgaatt ttcgtgtgat ttcgtggctc
     2881 ccctaataac cgcgaagcgg gtattatttc ccgtccttcc tttattcccg gctaattttt
     2941 tgccacgaat aggcacaaaa gcccacaaat ttttcccgct aagcctgata agactttatg
     3001 attaatgagg actcttcttc tttcgaaaag tcttaaaatc tttgcgggca atagaataac
     3061 cgcgaagcgg gaaatccttc ccggctaact tttctcgact aagttcgtta tacttgatta
     3121 atgaaatcta ccggtaaccg attaaaagac gaaacaagtc cttacttatt acaacatgct
     3181 cataatcctg tggactggta tccctggggg ccagaggcac tagagaaagc taaaaaagag
     3241 aataagccca tcctggtcag tattggctat gcggcctgtc attggtgcca tgtgatggag
     3301 cgggaaagtt ttgagaatga tgccacggca gagattatga atacccattt tgtcaatatc
     3361 aaaattgacc gggaggaacg tccggacctg gaccatattt acatggatgc ggtgcaggct
     3421 atgaccggaa gcgggggctg gccgctgaat gtttttttaa cacccgatac caaacccttt
     3481 tacggaggca catattttcc gccacagaaa gcgtttaacc ggccttcctg gacggaaacc
     3541 ctttatgggg tggcacaggc tttccgggaa cgaaggcatg agattgatgc ccaggcagag
     3601 aacctgacgg agcacctgct taatgccaat gcttttggta taaaaatgcc ggaggaggca
     3661 gatacattgt ttcatccgga aaagttgcag gagtgtttta ctcagtatat gaaaacggcc
     3721 gacaggcagt ggggcggttt tggaaaggct cccaagtttc cccagacctt ttccattcag
     3781 tttttattac gtcaccatta cttcaccggc gaacctgctg ccctggagca ggccctttta
     3841 tcattggata aaatgattga tggtggaatt tatgaccagg cagggggagg ttttgcccgt
     3901 tattctacag atgccgaatg gctggccccg cattttgaaa aaatgctgta tgacaatgcc
     3961 ttgcttgttt ctgtattaag tgaagcgtat caactgacgc ataaaccccg ttaccgggaa
     4021 gtgatcgacg agaccctggc ctttatagaa cgggagatga tgcatccggg agggggcttt
     4081 tattcagccc tggatgccga cagtgagggc gttgagggaa agttttatac ctggagccgc
     4141 caggaactgg agtcggaatt gggagaggag gcggccgctt tttgcgccta ttatgatgtg
     4201 tctgaggctg gcaactggga gcataccaat attctctgga tccggaagcc catggccgaa
     4261 gtggcgcaat cgttgaaaat ggaggaggcg gcgctttcgg aactcctggc aagggggcgt
     4321 aaaaggttga tggaaataag ggcaaaccgg attcgtcccc tcctggacga taaagtgctg
     4381 ctgggctgga atgcgttgat gaatacggct tattgcaagg cctatgaagc tacaggaaat
     4441 gaaaagtacc gggatcaggc gctgcgtaat atggactttt tactgtcggc tttcagagac
     4501 gaaacaggcc agtaccgcca tacctggaaa aatggtcagg cgcgatatcc ggcatttctg
     4561 gacgatctgg ccttcttact ccaggctatg ttacagttgc agcaggtaac cggagatctg
     4621 aattggctgg aaaggtcccg cgaattggta cggctgatta ttgaaaaata ccaggaaccc
     4681 ggaacccctt attttttcta tacgcctgat gatcaggaag atgtaattgt acgaaaaaaa
     4741 gaagtgtatg acggcgcttt accttcaggg aatgctgtga tggccatttg tttatatcac
     4801 atgtctgtaa tatttgatat gaaagagtgg aaagagcgaa gtctggatat gtaccgcatg
     4861 ctgggaaatg ctattacccg ttacccggtt tcttttgggg tatgggcgat gggggtacag
     4921 gaagtggtgg aaggaaccaa cgaaattgtg ctgaccgggg acaacccgga agtcctgatg
     4981 gccgatttgc tgggtagata tgtgccgaat aaagtagtgc tgacaaccgg gataaagaag
     5041 ttggatatcc cattactcga gggcagaaaa ggggagggta aaccttccat atacctctgc
     5101 cgtaataaca cctgccaagc gcccgtattt tccgcagata gccttatctc actgataaac
     5161 agggacaaaa aacagtaaat aaattttagt taatacaata aaataagctt catggccgtt
     5221 agaaaagtta ccgtgatttt tacggtttga tttctaaaaa taaattataa ccttgtgccg
     5281 gagaacagtt ctaaacatta tatttgccca atacccttta agtgtatgaa aatgagaaac
     5341 ctttattatg ccctgtatgc aaccgcgtgc atgactcttg tatccagttg tggaatcctg
     5401 ggtggtaaga aaaacaaagg cggcgccctg cccaatgacg gtcaggtgca cggagtagcc
     5461 cccggtggta agtatacact tcccaagcct ccaggcatgg tgtatgtgcc gcagggtaca
     5521 ttccatatgg gtcccagtga tgaggatcct gcgtatgcat tcagtgcacg gaaccgttcc
     5581 gtatcgatca gtggcttctg gatggatgcc accgaaatca ccaacaacga ataccgccag
     5641 tttgtaaact gggtacgtga ttctgtagta gcccgtaaga tggggtatgt aaaaacaggg
     5701 tcagacggca acgagaatgt tgactgggca aaaatgaaga ccatgaaatg gggtgatccc
     5761 aaggtcatgg agcagctcag cagcaccgat atcatccttc ctccggatga ccgtgtattc
     5821 ggtaaaaaag aaattgatcc ttccaaaatc gtgtttcatt ctgagacctt cgatctgaaa
     5881 gaagcagccc gccgggaaaa tgccggtaaa tcccgttcta cttttattgt aaagaaagat
     5941 gtggcgattt atcctgacac cctgtgctgg atccgtgact tctcctactc ttataatgag
     6001 ccgatgacca aaagatattt ctctcacccc gctttcggta actatccggt agttggtgtg
     6061 aactggaagc aggccaccgc tttctgcgaa tggagaaccc atttcctgaa tgcgttcctg
     6121 gattccaaga agagaacaca ggaatcagat ttccgcctgc ctaccgaagc gcagtgggaa
     6181 tatgcagccc gtggcggtcg ctcacaatcg atgttcccct ggggtaacta ttacctgcgt
     6241 aacaagaaag gatgcctgat ggccaacttc aaacctggtc gtggtaatta tccggaagac
     6301 ggtggtttct ataccgtacg ggcagatgga tactggccga atgattatgg tctgtactgt
     6361 atgtcaggta atgtggccga gtggacttct tctatctatt atgaaggccg caacaatttc
     6421 cagcatgata tgaaccctga tgttcgctgg aatgcaaaag atgatgatcc tcccctgatg
     6481 aagcgtaaaa tcatccgtgg tggcagctgg aaagatgtgg gatactacct gcagacaggt
     6541 accagtactt atgagtacca ggattccacc aaatcataca tcgggttccg ttgtgtgatc
     6601 gatcttcccg ccgctccgca gaaaggccgt cgctgattaa tatcgaaata acaatccaac
     6661 aatattgaga ggacagttaa acgaacctgt cctctcactt tctctgttca gtaaaataat
     6721 atcattaaaa ccacttttta aaaaatccaa ttatggcagc agcaattcca tctaaggtta
     6781 gtaaatgggt agacgtaggc gtatcattcg gtgcagcatt agttatctgg ggagccctcc
     6841 ggaaaatcac acacgcaggc gatgccgata cctggctttg gatcggtctg accaccgagg
     6901 caatcatctt ctctatctat ggtatattat atgccgttta tccggcagtt aaagacgctt
     6961 ctcatgacga agagcagttt tctgtagccg gtacaaaaag ccgtgcagcg ctgggtgcta
     7021 tggataaaat gctggctgaa tccgatattt ctcctgatac catgaaacgc ctgggcgatg
     7081 gtttcaaaca actgaatacc accgtttccg gtctgaccac tatcggcgat accatcaacg
     7141 caaccgctga cttcaccaac aaaacccgtg aagtaaccgg ccagctggaa aaagtaaaag
     7201 acgcttatac caccgctgct tcctcagttg gtgcgttcaa taccgctact gaaggtgcta
     7261 aagtattcca cgagcagatc caggtactga cgaagaacct gtcttccctg aacacgatct
     7321 acgaactgga gttacaggaa agtaataatc acctgaaagc gcttaactcg ttctacggta
     7381 aactgtctga aacatccaat gcgatgctga acagtgccga agatgccaaa aaggtacagg
     7441 atcagattgg tcacttagct actaacctgg gtcgtctgaa caacatttac ggtaacatgc
     7501 ttactgcaat gcagggtcgc agctaaaaca aactgtctga gccggaccac ctgaaccgtg
     7561 aataccggcc ggatattaaa agtcatcatt tctaatccta ttaaacctaa atagtcatgt
     7621 ctctacctaa agagccccgg cagaagatga tcaacatcat gtacctggtg cttaccgcac
     7681 tgctggcact gaatgtatca tctgagatcc tgaatgcctt ccggactgtt agacgaagcc
     7741 tggagaacag taatgccacg gtgacccaat ccaccgcgat cattatgaaa tccctggagg
     7801 acaagtcgaa cgatccggta accacggaaa gagctactcc ctggttccag aaagcacaga
     7861 aagtaactgc cacagcggat gcaattttta aatatatcga tggccttaag aatgatatca
     7921 tcaccaaagc gggtggtaaa cccggcgatc ctacggttac ttttaaagaa gataacctgg
     7981 acatcgttac aaaaatgttt gtacaaggta aagaaggaga caagctcaag caaaaactcg
     8041 agcagttcca gaaagatatc ctgggtatcg atcctgaaat cgacagtgcc tttaagaagt
     8101 cactcaatat tgacctttcc aatcctcccg gccaggatgg taaatccaag gcctgggacc
     8161 tggcttactt taacatggta ccgaccgttg ccggtctgac catcctgagt aagttccaga
     8221 acgacatcaa aggtgcggaa aataaagtcg tgtctttctg tcaccagaaa gtgggcgagg
     8281 taaaagtggt atttgactct tacgcagcta tcgtaggtca gaactccaat tacctgatgc
     8341 ccggacaaca actggaagta aaagccggta tcggggcctt cagtaaaacg tccaagccca
     8401 ctatcactat tggcggtaca actgtaccga ttggtgaaga agggtttgcc ctttacaaaa
     8461 ccgatgcggg tggtgtgggt gcacacagta tccccgttcg tatcagcttc tttaaccaga
     8521 ctactggtaa agatgaagta aaagaagtaa aagtggagta tacggttgga tctgccaacg
     8581 cctccattgc cctggataaa atgaacgtac tatatatcgg ggttgataac ccggtaacca
     8641 ttgctgccag tggtggtggt gatgataaaa tccagacttc tattaccgcc ggcggaagtc
     8701 ttacccgtgt aggtaatggg aaatacatcg cccgggttaa caacattact gatgattgta
     8761 aaatcactgt aactgtagat gggaaagtag ccggagcaag ccagttccgt gtacgtacca
     8821 tacccctggc ccaggcttat gtgggtggta aaccttctgg cgaaccctac caggccggtg
     8881 cctttaaggc acagggtggt gtgggagccg gtatcaagga tttccccttt gaactgagct
     8941 atgacgtggt aagtttcact tttacctgcg atacagatga tgatattttc tctcaaccgg
     9001 ttcagggtaa ttcctttgcc ccgatcagag cggctataaa ccagcacgtt aaggccgacc
     9061 ggatggtgac tatcgatgat attcgcgtaa aaggtcccga tggacgtact acaaaagccc
     9121 cttcactcgt ttattatatt aaataatgtg tgacatgaat atgaaatttg taaagtttgg
     9181 tttctgcctg gtgctgtcgg cctttttgtt gacgcctgcg gatgctcaac gggtgtctaa
     9241 aaagaagaaa gcggagaatg ccgctcaaac gcctcctgcc ggcaccgagc agcagcagaa
     9301 caacaatacg cagcctccct ccggttacga cccgctggct ggcctgccta tgacctatga
     9361 caccaccagt aatgatctga cgccgaagaa atctctgcgg aacgataacg cgtttgataa
     9421 gagcaatctg aatcagcgta ccccgctccc gtatgagcat cttcgctggg atgatgccct
     9481 ttacgctgag aaagtatggc gcgagctcga cctccgcgaa aaaatgaata aaacattcat
     9541 gtatgaagcc gtggatgata atggcagcca gatgtttgta aacctgttgc tgaaagcagt
     9601 tcagaacggt gaagtaaccg cgttcagtga tgaccgcttt accctgccga tggatgtgcc
     9661 cagtgtacag cagatcaccg ccggccgcct ggatacattc ccggtatatg atacaaaaga
     9721 tatctccaag atcgttggat ggactgtttc acgcgccagc tttgatgcca aatcagtgac
     9781 ccgtctccgt ttaaaagaag agtgggtatt tgaccgcgaa tccagccgca tgtttgtgcg
     9841 aatcctgggt atcggggtgc tgaaaacgga atatatgcca ggtactacaa aggaaagagg
     9901 tacatccgcc ctgttctggg tatactatcc cgatctgcgt ccgatgatgg ccaaagcgga
     9961 agtgtacaat ccaaaaaaca tgggccaaag ccgcatgacc tgggaagaac ttttcgaaag
    10021 ccgcatgttc agcagctata tcgtgaagtc taccctggat aactccagca acaaaatgat
    10081 ccggaactat atcaacgatc cgatcctccg cctgctggaa ggagaaaata tcaaagagaa
    10141 aatcttcaat ttcgaacagg atctctggtc ctactaattg aaatctgaac ttaatagaaa
    10201 ggctgtcctg cgggatggcc ttttttttta atcagttgtc ggatgtcagg tgtcagatgt
    10261 caggaaacag ccagtccaaa ctagtcggta gttagaagtc ggtagttagt agccaacagc
    10321 ccatactgag agcatatact taaacaacgt actgatgctg catttcgagc gtagcgagaa
    10381 atcttgtatt tattttttta ccctttacca agcttgtcat cccgacggag gagggacctt
    10441 gtatttaagc agttgttcgt tgttcgctgt taggtgttag gaaacagccc gtcaaaacca
    10501 gttagtagcc ctccttcgct aaagctacgg aggataaatc ggtagttagt agcctgcctg
    10561 ccggcaaagg caggtcaaca gccaatgctg atagcatata tttgaacaac gaactgttgc
    10621 tgctatttcg agcgctactc cttcgtttat ctacggagtg caacgagcga gaaatcttgt
    10681 atttaagctg gtgttcgttg taagctgtta ggtgttaggg gaacagcccg ttctgagaga
    10741 aggtgtcggg tgtcaggtgt cgggtgtcgg ggaacagcca gtcaaaacca gtcggtagtt
    10801 tgaagtcggt agttagtagt caacagcctc tcctgaggag cttgaatcta attaaactgc
    10861 tgccactcgt tttacgtcat gcccttcgta cttcgtacct cgtacttatc ttcaggtgtt
    10921 gattctacac ttatcctgtg gactataaaa tctcttcaaa aagtcaagct cattgttgta
    10981 aaagcttcat tactaagtaa aaaaagttgt caaaatcgta aaagtcctat aggaaacctg
    11041 aaaaaaagtg gtaccttcgt catggttttt cataggatat tggattttaa aaacggggct
    11101 gaatttctat tccggcccca ctttttttta tataggtcct cannnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnagttttaa ggtgggactg ttatagggca gtttgagcga
    11281 ctgggtatcg ccggtaaaga aaatttcctc ttcattcttg gcaaggccaa ccagggtggt
    11341 tcttccttcc agttccagtt cggttatggc ttcattagcg gcgcttagct ggcctttgcc
    11401 accgtcgatg ataactaact ggggaaaatc ctgtccctct tcagagagcc gcttataacg
    11461 tctgtaaaca gcttctttca tagtagcaaa atcgttaata ccttccaccg tttttacatt
    11521 aaaatgccgg tagtcttttt taaaaggttg cgcattccgg aaacagacca tggccgaaac
    11581 cgggtaactg ccctggaagt tggagttatc gaaacactca atatgaacgg gcagatcact
    11641 cagggtcagg tcttcctgca gttgttccag caacgctttg ccggcttctt cttttgaatt
    11701 cagttttaac cgttccttcg cctgcaattc atcgataaag taacgggcat tcttttcggc
    11761 cagttccagc aatttctttt tatctcctcc ccggggtaca gtgacgatca tatcttcctg
    11821 cggaaaatca atcgggatgg gcacgactat ttcgcgggca tcactgttga atgtgctccg
    11881 taaggcagct actgaaaaag ccagtacttc ttcgggtgtt tcatccagcg gggtatccac
    11941 tttattggtc tgggtctgga taatggagcc gttacgcacc atcaggtagt tgacataagc
    12001 aatctctttt tcctgtagca ggtagaaaac atcgagatca gctgatttca ggccggccac
    12061 caccgagcgt gactggtaat tttccaggta gtcaattttc ttccggacga gttccgcttt
    12121 ttcaaaagcc agttcagcgg catgggcctt catctcctct ttgaaatgcc ggatcaccgg
    12181 ggccaggttg ccgcggagca ggttgcggag ctgactgata tattcttcat aatccgccag
    12241 ggtctgtaat ccttcacagg gacctttgca gttacccagg tgatattcca ggcagacttt
    12301 gaatttcttt ttttcaatat ttccgggagt caggttcagg ttgcaattgc gcagctggat
    12361 ggtttgtttg ataaactcca ggagttccct tacccgtttc accgaagtat agggacccag
    12421 gtattccgat ccatcgtctt tcttctgccg ggtcaggaaa acccgtggga agggttcgtt
    12481 tttgataaca ataaaaggat aggtcttatc gtctttcagg ttgatattga accgcggctg
    12541 gtattgtttg atcagggagt tttccaacag gaaggcatcc tgttcagaat tgacaatcgt
    12601 aaactcgatc cgggcaatcc gctggaccag ctcatgcgtt ttatacgtgg taaaggtttt
    12661 tgaaaaatag gaactcaccc gtttcctcag gtgtttggcc ttacccacat agaggagttg
    12721 ttcctcgctg tcaaagtatt tatagatccc tggctgaacc ggaatcgtag aggcgatcag
    12781 atgaaattcg gccgcggtca tgggatgttt tcttcattcc aggttacccg attagtactg
    12841 gtgatttccg gctggcccgg ttgctgggta ctggtgacga ccaggaagct ccggtcggtt
    12901 tgccagagca tctttttctg caggaatccg tcccgattat tgatgatccg gtctgcgata
    12961 atgttagtga ctttattact gccatcttcc tttagttcct ggctgatcag caattcaatt
    13021 ttcttgaatt cctctttttt gacatcaagg ggggtataag taatgatcac ccggcggtag
    13081 agggtatcgt acattttgtc ttctttaaag cgggccgcga cttcagggtc tgtcaggtcc
    13141 ggtagctcca ggaattcacg ggcggtttcc ctgaattttt cacgggggat ataagtggta
    13201 tcctggtgca agctgtcttt ggaaaccacc tgaacaatag aatacaggga ggtatccaca
    13261 tgggccacct gtttttcaat cagcgaaacc acggagataa atcccttgct ctccgctttt
    13321 tttttctcct tacaggaaaa caggaagagg gtcagcaagg gaagcccggc aaggaacatt
    13381 tttttcatgc tgctaaatta attgaaacca cattaactgc acgacctgac atggaaaacc
    13441 cgccagttga aggcgggttt tccatagtaa gcagttttca gcattttatt aacggttgag
    13501 catgatgccc agtttgaatc catagtcaaa caggtgttcc tgtaccgggt attctttttt
    13561 gaagctggac atggtctggt agcggacctg tggtccgatc cgccaggcgg ttttccctac
    13621 attgaaaccg gcaaaggttt caaatccggc attgacattc catttgcggg tgagggacgg
    13681 tacttccata tagtttttgt aatcggtaga gattacgtaa gcccggttac ccagtacata
    13741 agtgggttgt aatgtggcgg ccacacccac aaaggctctt cttgttttaa aagctttgta
    13801 ttcgagacct accggaacag aaaccgaata gtagaagttc cgcagccagt tggctttatt
    13861 ggctctgttc accccgacga tccggtagtt ggtaaccgtg gaaactgtgc tggttccgcc
    13921 ataatcatta ctcagggcca ccgtagcgat ttcactcgga tagttgtagg cccagatatc
    13981 atacttgctg atattgaact ggaggccggt gagcagggat aattttttag tcaggggata
    14041 ggccatggac acacccagtt ggaggcccag atcggggcga tgggtgacca ggttattcag
    14101 gtccggggaa taggtgagcg cagttggact catattcgaa cgggctgaat tgataaattg
    14161 tttgttttcg ttgagcttac ggtacgtaac cgttggcgtt atgtatagct gccatttggc
    14221 tttccgtgtc ttacgggcca cactatatga attgatcaca ctttcgatgg tcaggggata
    14281 aacctgatct tcactgttta attcaggtgc agtttccgtt tgtttttcat tcatcccgga
    14341 aattgctgcc ggatcagtaa tcaggtcgtc agcgatagac ggaataacgg gttttgctgc
    14401 cacaaccact gcaggtgctt cattcaggtt ttcgggttgc cgcggggctg tccgggtaac
    14461 gatggcggat ggattcctgg ccggattgac cggtgcagcc gggaagctgg cacttatatt
    14521 tccaaggctg gtactgacag gggcaagggc tacagtgctg gccttatcca cgcctgtaat
    14581 atctgcagtt gattcagggc tttccaaaac ttcagtggcc aggaagatat ttttttcctg
    14641 attgccgggt gaggcaatcg gtggtttgct gatgcgattg gctttaggtg tggtgaccag
    14701 ctcggaagga ggtagtgttg ctgtttcttt tttcgcttcg ggtttacgag ccgcgattat
    14761 ttgttctttt ttattaccgg aagtagtggt cagcataatc caggtaacgg taccggtagt
    14821 gagtaaaagt aaagcgagcc agagtccatg ccagcggcga cgggtatgca gtgtgttatg
    14881 aatgccatcc cacacatttt cagaaggaaa catccggtac tggtcagcat tttgcttcag
    14941 aaaccgctcg aaatcgttat tattgaaatt actctccata accagtcagc aattaagggt
    15001 acgtttatca tcagcggcgg cttacggcgg ccagccagtt gaactcctgt tttggtctgt
    15061 gcaatattct ttttcttact aaaatatctt ccagcatggc tttggcccgg gtatactgac
    15121 tgcggctggt actttcttca atatccagca tgtttgaaat ttcccggtga ctataccctt
    15181 ccacggcgta gagattcagt accgtccggt aaccaatcgg caacaaccgg atacattcca
    15241 ccacctgttt ggcctggata atggctggaa ccgactcttc cctgacctga acacccgttg
    15301 catggataat gtccacgctt tcattgaact tcttattctt cttcaggata ttaatacagg
    15361 tatgtaccat tatcctccgg atccaaccct caaatgctcc ccggttttca aaagtgtgca
    15421 tctgtaaaaa gaccttaatg aaaccttcct ggagcatatc ttccgcatct tcccggttct
    15481 gtgcaaaacg ataacatact gccagcattt ttgaactata ccggttgtac agttcacgct
    15541 gggctgatgc ctcatttttc aaacaacctt ttaaaatggc ttcctcggtc ataactaccc
    15601 tttggttgcc tgtaaattag acattttttt gaaagggttg ctgcatccgt ttaaaaattt
    15661 ttaagtgttt aacaattaaa aaaaagggtg attgtgttga caaccaccct tttaaagaaa
    15721 aaatatgaaa ttgaatattt cttacataat aagaatcagc ggacatgcgg aactcatttt
    15781 tgagttttcc tgcttcgccg gatagcacct gggccatacc agagccagaa accagtaaat
    15841 gtaaaaacaa acaaggcaga tcccataacg gacgtataca ccagtttgaa aaaatccccg
    15901 cttgttccgg aataataatc aaggatggat ccgtcatgta ctttttcaat aaagtcagat
    15961 ctccttttgt cgatatgaag gagtttcccg gttgccccat ccatttgtaa tcccaggaat
    16021 tgtccgttga aaacaaattt gacgacacct ttgtctttcc gtatatcaat tctttcgagt
    16081 tcggttgaag aagcagggga aactgaatca tgcatgtaca ggcaggcaga taaatataga
    16141 ctatctaaag tgagccattc ggacaggttt gttgagctgc ctttataggt atctggcaat
    16201 agatatcctc cgctgttttt tttccatcca agtaacaagc cggttattga cataataaag
    16261 aagaaaacaa acaatagcat acctgtggtc cggtgtatct tcctgtaaag ccgtagccgc
    16321 tctgcctggt gtactctttt gttgtgcgga tcagtatgtg aagctttcag tttcatacct
    16381 gttgatttga atgaaggcgc aggatttgtc cctgcgcctt tgaataatta caacgacgcc
    16441 ctgacagaga ggataaaatt actcccagct gccacaattc ctgatgagta aggtctgtag
    16501 cgttgattgc tcatattttc ccaaccggca gtgagcatca actgttgtat taactggtac
    16561 gaagctttca gattcaaggt ataccaaccc ggagcatacg gtttcccgga tgcatctttt
    16621 gcataaatat ctgtcttcgc cttttcactc ggggccagat cggcattgct gatctggctg
    16681 ttatactgac agttcagttc agcagttatt ttttttgtcc gtaaacggaa agcggtgctg
    16741 ccatagaatg gcggagcatg acgcaggggt acctgttcat tttttacgtc atccgtttct
    16801 ttgccgctga tccagttagc atgtgataca aaggagaaag attttgtaaa aaaaacttcc
    16861 gcgctgagct gtataccgtt aaccgtagcc ctggccacat tttgtaatgc ttccacacgg
    16921 ctcagtacat tgttgaacag gatactgtcc tgtccgttga atgtggtggg gcgtcttaca
    16981 attgcattgg tgagcagggt atgaaatccg gtaatttcaa aaaggtattt tccaggacta
    17041 ttccggataa tgcctgcttc aaagttccag gcatattccg gtgtcagctc cggattgggt
    17101 atggtaacat ttcccggaac actttcaaac aatttgccaa tatcgtccac atttggcatc
    17161 cggtatccgg tagagatatt accattgagc tgccatccct cagccggacg gaatacaagg
    17221 ccggcattgc cggtcagtgc accatcccgg agttcagctt gcttatacgg gaacttgata
    17281 aaagtggtat caaatgtggc atcgagtgta ttataacaca aattgactct cggcgattga
    17341 cattgctgct aataaagtcg ttttccttgc agcagaggtg tttcggttaa tctgccgcgg
    17401 gtattatcgg aatcaggcaa gaaatttttg ggttttaaga agcggtcaaa agcggtcaat
    17461 tttcgcgatt gaaaatcttg gcaatatttc ataagatctt ttttggccaa tagatatgga
    17521 ttgcgtattc aataaaggaa accgacttga tagaatgagg cccttgtggt ttactgccgc
    17581 agagcaaacg ggcgatggtg tactgctcgg aatcggtgtc ctgaaactcg tcgatgagca
    17641 ga
//
LOCUS       DAOPMW010000028        17259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3210_length_17259_cov_9.787608, whole genome shotgun sequence.
ACCESSION   DAOPMW010000028 DAOPMW010000000
VERSION     DAOPMW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17259
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_17259_cov_9.787608"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PLU11_02750"
     CDS             <1..1334
                     /locus_tag="PLU11_02750"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPN58057.1"
                     /translation="GDGVITGYGTVNGRLVYVFAQDFTVFGGALSETHAEKICRIMDM
                     AMKAGAPMVGLNDSGGARIQEGVRSLGGYADIFYRNVQSSGVIPQISAIMGPCAGGAV
                     YSPAMTDFTIMVENTSYMFVTGPNVVKTVTNEEVSSEELGGASTHSTKSGVTHLTALN
                     DMDCINQVKTMLSYMPQNCEDVVVKHPYTMGDETREILETIVPASANQPYDIRSVING
                     ICDEESFFEVHKYYADNIVVGFARLAGRSIGIVANQPMCLAGVLDIDSSKKGARFTRF
                     CDCFNIPLLVLVDVPGFLPGTDQEWNGIITNGAKLLYALSEATVPRCTVITRKAYGGA
                     YDVMNSKHIGADMNYAWPSAEIAVMGAKGASEIIFKSEIAAAEDPAKKLLEKEAEYAE
                     LFANPYTAAERGFIDEVIEPRETRKKLIKAFAMLENKVAKRPRKKHGNIPL"
     gene            complement(1406..2197)
                     /locus_tag="PLU11_02755"
     CDS             complement(1406..2197)
                     /locus_tag="PLU11_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPN58058.1"
                     /translation="METILLILFVLAILTLLLSVAFRTGSKKQTTLPPFPEHWRSLLD
                     EQSVFYQELDTNGKTAFEKRIQAFLQQTRVTGVNTPVEELDKVLIGASAIIPIFSFPD
                     WEYINLREVLLYPDSFNHDFEQEGADRSILGMVGEGALNGVMVLSQHELRQAFLNKTS
                     KTNTAIHEFVHLVDKTDGTVDGIPEILMQRQYVLPWLQAMQKEIKKILADKSDINPYG
                     ATNEAEFFAVVAEYFFERPDLLERKNPELYQLLTSIFRQDSNRPA"
     gene            complement(2235..2585)
                     /locus_tag="PLU11_02760"
     CDS             complement(2235..2585)
                     /locus_tag="PLU11_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58059.1"
                     /translation="MKLMQHHMILNWTGRISGILITAFFAAFFIGEGVPEILNGKGKE
                     LLEFIPFTLLSLTGFIVAWYSPLWGGRLLLAGALLLVGYFIFQQDLIMALIYGLPSGL
                     TGLCFIAAANKELI"
     gene            complement(2606..3265)
                     /locus_tag="PLU11_02765"
     CDS             complement(2606..3265)
                     /locus_tag="PLU11_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58060.1"
                     /translation="MKQLFYMAVLLLFLLPGCSSSRITSSWKADNVQPQAFKKIIVLG
                     LIREQDRHLRESMEQHLVGELLTLGYDATCSCDEYNPKAFENMTEEQALAKLKSSGVD
                     AVLTVVLLDKTQERYYVPGRVQYTPYRIYYNRFWGYSRTMYGRIYTEGYYTEDTKYFW
                     ETNLYALEKNELLYSAQSQSFDPASTEKMGDEHGRLITLNMLKKNVLINLKEQPVKKA
                     M"
     gene            3593..4966
                     /locus_tag="PLU11_02770"
     CDS             3593..4966
                     /locus_tag="PLU11_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPN58061.1"
                     /translation="MISVIIPALNEEKTVGDVVKLALSSVGVTEVIVVDDKSLDATVA
                     TAKAAGAVVITSTKLGKGTSMKDGILYANNELIVFLDADIITYPENIISLLTAPLVKD
                     TADFSKSFFSRQAGRVTELVAKPLLSILYPDFPSFWQPLSGMIAGKKSMFKKIHFEEG
                     YGVDIGILIDMYQQGARIAEVNIGHIENRMQSLEQLGKMSREVAAVILKKSSHKGENN
                     YETLEHIQLIREQMEFAIRESSRSLKKVAIFDMDNTLLRASFIHTAAEHFGFRKELIE
                     IVTTQNNPFIRTKLIARLLKGRDFSELIRLADSIPVTDHTSEIIQLLRDNGFVVGIIS
                     DSYDCITNHLKNRFGFDFSLANELEFSKSVATGEVKIPSFFLSHEGSMCNHEYCKTHA
                     LTHICNRFSVPVSDTLAIGDSENDICIIKKAGIGVSFCSSNQLLDAVADFIVKEADFN
                     LLTPVIE"
     gene            4972..6246
                     /gene="nhaD"
                     /locus_tag="PLU11_02775"
     CDS             4972..6246
                     /gene="nhaD"
                     /locus_tag="PLU11_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HPN58062.1"
                     /translation="MFIFFLIAVFVIGYIAIAFEHPLRLNKAASAMITGVLCWTIYMF
                     SSASAETVTDELLHHLGEIASILFFLLGAMTIVELIDTHNGFEFITRHITTRSKSRLL
                     IIVVVITFFLSALLDNLTTAIVMASLCARLLKNKEDKLWFCGMIVISANAGGAWSPLG
                     DVTTTMLWIGGQITALNIMKQLILPSLVVCALPLALVAWRFRGQQIDRLEEKDDQTKA
                     EKKDGLLILFSGIGFLVFVPVFKTLTHLPPFMGMLLALGLMWVITTFLHKDKEPEQLE
                     KLTVARALQKVDSPSILFFLGILLAVSSLQSAGLLQTGASWLSNTLGNDYMIGSFLGL
                     LSAVVDNVPLVAASQGMYDLQTYPTDHPFWEFLALTTGTGGSAIIIGSAAGVAVMGIE
                     KIDFMWYLRNISWLALIGFAGGIAVFLLQMQF"
     gene            6491..8053
                     /locus_tag="PLU11_02780"
     CDS             6491..8053
                     /locus_tag="PLU11_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPN58063.1"
                     /translation="MFRGFIERGIAMVLRSIARRSSFIPLSHTYLHVITSSVTVGFGG
                     SAGLEAPIVATGSAAGSTIGRISDLTYQERTLLIACGAAAGIAAVFNAPIAGVIFAVE
                     ILLTEMLVSHFIPLIISSVTGVLCSKVILSEASLFNFRLTQDFDYRNVPYYLLLGILA
                     GFISLYYARAFKSTESRLHHWKLPPLMKALTGGILLAGLYFILPPLFGEGYESVKVVA
                     SGTFNSLTGTSSPLSQLGDTAGIIVFAAMIVLLKPVAAAITIGSGGNGGNFAPSLFTG
                     SYLGFFFSKLLNATPWIKIPVGNFSLVGMAGVLSGVMYSPLTAIFLIAEITNGYELFI
                     PLMIVSSISFFIVKSYEPYSMELKKLAMEGEVFTHKKEKNILTSFQLSDILHDNFETI
                     SVDKKLGDLVEIIKKSEKNIYAVVDSKGKFAGIIELNDIKQRLFDSSQFDIVPVRTLM
                     KKPPAVLQEDEHMHSVMEKFDLTRSWFLPVLNKDKIFVGFISKTKIFYKYREILSASA
                     DIYEQESGNKPG"
     gene            complement(8059..8619)
                     /locus_tag="PLU11_02785"
     CDS             complement(8059..8619)
                     /locus_tag="PLU11_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58064.1"
                     /translation="MGLSIHYSGSIKDRSLVPQLGAEMKDICESLGWDYHYFEADEKD
                     PLEGIYFAPEECEPLFLTFTPEGRLLSPISHITREMLVEHGLDPELIYTISTKTQYAG
                     IDAHVALIRLFRYLSKKYFSHFEMTDEGGYWESNDLNVLKNQFAAYERAIQTFVDALQ
                     GMERIPGESPDSLASRIEELLKKKEG"
     gene            8936..9130
                     /locus_tag="PLU11_02790"
     CDS             8936..9130
                     /locus_tag="PLU11_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58065.1"
                     /translation="MKKYFFIIKSLVAGLIVLFVLEGCGTEYHYYQQHKSHSPKYMKR
                     QVKYKKYQHHDNGVEIKIRH"
     gene            9280..10782
                     /locus_tag="PLU11_02795"
     CDS             9280..10782
                     /locus_tag="PLU11_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPN58066.1"
                     /translation="MKRSCWIVLFILMYSALAAQTDTIKKFPNGNILLRKVAGMQLLS
                     PSGQNLGSWTETMHNNKQGGRFVSIVSKTDSLGKRKSGVFDQQNGEFVLPVEFDQLSP
                     TDYGKGFYTTRIGKKYGFYSVPAGKRLPPIYDFTGRHVNPAWFIVAVSGEYNCGYVYD
                     EKLALVDSIPGMKSVNSRIVSGNREWYQINMADGQGLLDKENKLLYRNEWKHIHDVKG
                     MTAIVNNGKAIGLFDLATGKLVKPYSFTDYWPDIFGSQGFLKNGNQWVLIDQLGKQLF
                     TFKAEEVEFSHRGETGGFYFKQKGLWGVMNNKGKIVRSPVWESVSPGLAGGFTGKYPG
                     KPDTYYNGVYKEINGEKVLTGIRQLNTEEVTAAYPDQAVWVREEPKEVIEMMNIPAPV
                     RHEENEDHIFVKMEVDPAFTKTGEVEKTVLRQLVDAYKKEFQIKTTGTVQLKLVVEKD
                     GKISSTIVVSSPDPLLTEASKKLLGNITGWKPGIQNGRNVRGEKTLVLEW"
     gene            complement(10792..12273)
                     /locus_tag="PLU11_02800"
     CDS             complement(10792..12273)
                     /locus_tag="PLU11_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPN58067.1"
                     /translation="MHIPSLIKINKNKPEPIYLQVAECLSDAIRNGILPGGLKLPGVK
                     KMAQQTGLHSRTIEQVYEELDAQGWVTVKPHSGTYITDKPPVLSATGKKEVLSAAEHK
                     ATFPFRKIGLPPRQTGYSKEGKKIYDIILNDGQPDIGYMPAVELEKNHRLLIRKKEHS
                     RLFNKENAWGSFELRKALSRFLNDTRGLNTGTSNLLISKGNQFSIFLVLSLTMTEQDS
                     IVFAEHNYSGVYSICRYLNLNIETVKTDQDGISLGELGKICKRKKIKAVYITPHIHYP
                     TCVKLSEERRKQLLQLAEQYNFMILEDDYDFDFHYTNAPGFPVAAYDNQKRVVYIGGL
                     NRLISPTLRISYIAGPEDFLSELAKLKVSIDKFSDSLLEEALALTLKEAVIRRYRNKA
                     VKYYMHKRDFLLRLLDKNLAGCCTYHAPEGGMAVWIEFNREFPVSDIHRLCMKKGLLL
                     PERDDLYTYPPESNGIRIGFGNSTKAELSNAIQIIREVIAVVK"
     gene            12341..12823
                     /locus_tag="PLU11_02805"
     CDS             12341..12823
                     /locus_tag="PLU11_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN58068.1"
                     /translation="MKIGFVMVVCGIAILSGSFKINKIMNENNGTGAVQFRIARPTDK
                     LEEVVDFYKTVLGLEEIGAFKQHEGYDGVMLGLPGFDYHLEFTQHTDGSPCPAPTKDN
                     LLVLYFDTPLKYNRAVERALQLGHQPVEPENSYWKGKSETFEDPDGWRVVFFNGVYKP
                     "
     gene            12829..13494
                     /locus_tag="PLU11_02810"
     CDS             12829..13494
                     /locus_tag="PLU11_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN58069.1"
                     /translation="MRIQKLVLQTTDLQRQYSFYIEKFNFQVVTADQDHFSFSAGASL
                     VEFRAYSGKDIFPYHLAFTIPAGQLIKAADYLAAKGINLVKMGNQTIFQFESWNATSL
                     YFRDPDENILELIVRFNLGLKNGSVHFSPSDIIQVSEIGIVADDPQAFSSYITEQSSL
                     PVWKMAGNDFIAMGDEEGLLIVVQKNRNWYPTDTPARAMPVYIHSPAFNRSIQADPYL
                     FAP"
     gene            13626..14186
                     /locus_tag="PLU11_02815"
     CDS             13626..14186
                     /locus_tag="PLU11_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58070.1"
                     /translation="MKKTIFLLLLTVFSVSGQAQKRQPSVAGYGAAVAELTSIDKKAA
                     LNLGGYGGVLINHRFLLGASGNNILFSKTINGSKTDFQFNYYGLITEYKFFPGSFVHF
                     SAGLTSALGWLENDILTADKKKRMDGDYVYVIQPRLALELRITSFMQGKLYGNYRFTG
                     NTKSRYFTPRNLDGAGAGAALVFGSF"
     gene            14287..14763
                     /locus_tag="PLU11_02820"
     CDS             14287..14763
                     /locus_tag="PLU11_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPN58071.1"
                     /translation="MEKSIQTEIIIHASREKVWEVLTDFSSYALWNPFMVSASGKLAK
                     GQRLKIQLQNGSSTMTFSPAVRSVIPGFSFSWEGRLFFKGLFDGYHFFKIIQNSSNQV
                     KLVQGERFSGIFSRMIFHKIGASTAVNFDRMNRELKKRAEMKEIRATNHIKITGDA"
     gene            14756..15811
                     /locus_tag="PLU11_02825"
     CDS             14756..15811
                     /locus_tag="PLU11_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPN58072.1"
                     /translation="MHKQRMRYMDNLKVMLTCLVVAHHAGQAYGPSGGVWVVKEADAL
                     PWLRIFFYVNASFMMGLYFFISGYFMVVSLEKKTTGRFIKDRLKRLGIPLLFFTFLVF
                     LPFNYVVSGGEGNIFFVLADTYLHQPPLATGHLWFIASLLLYTFLYLAIRRMVRPVPA
                     RGFIFRPYYIAVYIFLLGLVTVFVRSYYPIDQWRTWLIPLEIAHVPQYLSLFLLGILF
                     NQFCWLEQLKLRYGFLVLIITAAIPVLPGTDLLDTTWIASFEESGLCVGLSIILLVIF
                     RNYYNQESAITLSFSQNSYGIYLFHLLIVIALQQLLVNWPAAAEFKFPLVVVLGVFVS
                     FMLSRLLRKIPLVRRII"
     gene            15993..>17259
                     /locus_tag="PLU11_02830"
     CDS             15993..>17259
                     /locus_tag="PLU11_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPN58073.1"
                     /translation="MICRSGLLLLFSLHCSLILPAQPLSTADQIPANSFNPSTLLWYD
                     QPARKWEEALPVGNGRLGAMVFGKNKEERIQLNEETYWSGGPYSTVVKGGHNYLKEIQ
                     QKVFDGKFLEAHNLFGRELMGYPVEQQKYQSLGNLHLFFNNQDSVTAYKRWLDLATGI
                     AGVSYEAAGVRYQRETFASAPGQVIVVRLTASKPGSISFTANLRGERNQAHSNYATDY
                     FRMDPYGQDGLILTGKSADYMGVEGKLRYEARIKAVNEGGTITTNGVDLNVSKADAVT
                     LYFAAATNFVNYKDVSGDPHKRVEDCFNKLGNQSFTSILNEAVIDHSSYFNRVSLKLP
                     VNSQSFLPVTERIKRIQSAPDPAMAALSYQFGRYLMIASSRPGTQPANLQGIWNDNMN
                     PSWDSKYTTNINTEMNYWPVESGNLSECAE"
BASE COUNT         4546 a         3669 c         4131 g         4913 t
ORIGIN      
        1 atggcgatgg agtgatcacc ggttatggta cagtaaatgg cagactcgtt tatgtgtttg
       61 cccaggactt caccgtattt ggcggggcac tttcagaaac ccatgcggag aaaatctgcc
      121 ggatcatgga tatggctatg aaagccggtg caccgatggt aggtctgaat gactccgggg
      181 gtgcccgtat acaggaaggc gtacgttcac tgggtggata tgccgatatt ttttaccgta
      241 atgtgcagtc ctccggtgtg attccccaga tctccgcgat catgggccca tgcgccggcg
      301 gagccgttta ttctccggcc atgaccgatt ttaccatcat ggtggaaaac accagttata
      361 tgtttgtcac gggtcccaat gtagtgaaaa ccgtgaccaa cgaagaagtc agttccgaag
      421 aactaggagg agcttctacc cattccacca agtcaggggt aacacacctg accgccctga
      481 atgatatgga ctgcatcaac caggtgaaga ccatgctgag ctatatgccg caaaactgtg
      541 aggatgtggt agtgaaacac ccctatacaa tgggtgatga aacaagggaa atactcgaga
      601 ctattgtacc cgccagtgcc aaccaaccgt atgatatccg cagtgtgatc aatggcatct
      661 gtgatgagga atctttcttt gaagtacata aatactatgc ggataatatt gtagtgggtt
      721 ttgcgcgtct ggccggccgt agtatcggca tcgtggcgaa tcagccaatg tgcctggccg
      781 gggtactgga tatcgacagt tccaagaaag gcgcccggtt tacccgcttc tgtgattgtt
      841 ttaatattcc gctgctggtg ttggtggatg tgccgggctt ccttccgggt accgatcagg
      901 aatggaatgg tattattacc aatggcgcca aactgctgta tgcgttgagt gaagcaacag
      961 taccacgttg taccgtgatc acccgaaaag cctatggagg agcctatgat gtgatgaatt
     1021 ccaagcatat tggggccgac atgaactatg cctggcccag tgctgaaatc gcagtgatgg
     1081 gagccaaggg agccagtgaa atcattttca aaagtgaaat tgctgcggct gaagatccgg
     1141 ctaaaaaact gctcgagaaa gaggccgagt atgcggaact ctttgccaac ccgtataccg
     1201 ctgcggaaag aggatttatt gatgaagtga tagagcccag ggaaacccgg aaaaaactga
     1261 tcaaggcttt tgccatgctg gagaataaag tggccaaacg cccgagaaaa aaacacggga
     1321 atattccttt atagttcatt atttaaaagg gacagctgct attgcttatg caatagcagc
     1381 tgtccctttt tatgcatttt atctgctaag cggggcgatt cgaatcttga cgaaagatgg
     1441 atgtgagcag ctgatacagt tccggatttt tccgttccag taaatccggg cgttcaaaaa
     1501 agtattccgc cactacggca aaaaactcgg cttcattggt agctccatac gggttgatat
     1561 ccgatttatc agccagtatc tttttgattt ctttttgcat ggcctgcaac cagggcaaca
     1621 cgtattgccg ctgcatcagt atttccggaa tgccgtccac tgtaccatcc gttttatcca
     1681 cgagatgaac aaattcatgg atggcggtat tggtcttact ggttttattg agaaaagcct
     1741 gtcttaattc atgttgcgat aaaaccatca cgccgttcag cgctccttcg cccaccatac
     1801 ccagtatgga acggtctgcc ccctcttgtt caaaatcatg attaaaggaa tccggataaa
     1861 gcaatacctc ccgcaagttg atatattccc agtccgggaa tgaaaaaatc gggatgatgg
     1921 cactggctcc gatcagaact ttgtccaatt cctccacagg ggtattgacc ccggtaacac
     1981 gggtttgttg cagaaaagcc tgtattctct tttcaaaagc cgttttaccg tttgtgtcca
     2041 gttcctggta gaaaacggat tgttcatcca gcagggagcg ccagtgttcc gggaaagggg
     2101 gaagggtagt ttgtttttta gaaccagtac ggaatgccac actcagcaaa agcgttagga
     2161 tcgccaggac aaacaggata agtaaaatgg tctccatcgg catgaatata gaaattaccg
     2221 gggagattcg gggctcagat cagttcttta tttgccgccg ctataaaaca aagaccggtc
     2281 agcccggaag gcagtccata tatcaaagcc attatcaggt cttgttggaa aatgaaatag
     2341 ccaaccagca gcagcgcccc ggccagcagg agcctgcctc cccagagcgg actataccag
     2401 gccactataa aaccggtcag gctaagtaaa gtgaatggaa taaattccag cagctctttt
     2461 ccttttccgt ttaatatttc gggcactcct tctccaataa agaaggcggc aaaaaaggcc
     2521 gtaatcagta ttccactgat acggcctgtc cagttcagga tcatgtgatg ttgcatgagt
     2581 ttcattttac cttattatac tttgatcaca tggctttttt caccggttgt tctttcaggt
     2641 tgattagtac attttttttc agcatattca gggtgatcaa tcttccatgt tcatctccca
     2701 ttttttcagt ggaggccgga tcaaaggact ggctctgtgc ggaataaagc aattcatttt
     2761 tttccagcgc atagagattg gtttcccaga aatattttgt gtcctctgta taatatcctt
     2821 ccgtgtagat ccgcccgtac atcgtgcggg aataccccca gaaacggtta tagtaaatgc
     2881 ggtaaggtgt atattgtaca cggccaggga cataataccg ttcctgggtt ttatccagta
     2941 acaccactgt aagtacggca tccacccctg aactttttaa tttggccagc gcctgttctt
     3001 ccgtcatatt ttcaaaggct tttggattgt attcatcaca ggaacaggtg gcgtcgtaac
     3061 ccaaggtaag gagttcacca accaggtgtt gttccatact ctcgcggagg tgacggtctt
     3121 gttcgcggat caatcccaat acaatgattt tcttaaacgc ctgcggctgt acattgtctg
     3181 ctttccagga gctggtgata cgggaagaac tgcatccagg caggaggaat aacaacagta
     3241 cagccatgta gaatagctgt ttcatgttat tatattttat tcactcttaa gttagttcat
     3301 attgtaagcg ataactatga gttatctcat ttgtcagaat gagcgtcctc aatcagcaaa
     3361 gctttgaatg tatactatat aacttattga atgataatct ggtgaatggg agcacgctac
     3421 acattacaat tgctggttaa tgtaatatgg gtatatttat ctttctttcc tggccgtaaa
     3481 gcagtatata catgcattac tgagaatgtc tgctgcaaaa tcagtctgta ccagtttgaa
     3541 taactggtta cggtcttatc aggtaatatc ttttaacatt aaatataaag ccatgataag
     3601 tgtaattatc cctgctttaa atgaggagaa aaccgtaggt gatgtggtta agctggcttt
     3661 gtcatctgtg ggtgtcacgg aggtaatagt cgtagacgat aagtcactcg atgccacggt
     3721 tgcaaccgcc aaagcagccg gggcggtggt gatcaccagt accaagttgg gcaaaggtac
     3781 ctccatgaag gacgggatac tgtatgcgaa taacgaactg attgtttttc tggatgcgga
     3841 tatcatcact tatccggaga atatcatatc actgcttacg gctcctttgg tgaaggatac
     3901 ggcagatttt agcaaatcat ttttttcgcg gcaggccggc cgggtcacgg agttggtggc
     3961 gaaaccgctt ttatccattt tgtacccgga ttttccttca ttctggcaac cgttaagtgg
     4021 tatgattgcc ggaaagaaga gtatgtttaa gaaaattcac tttgaggaag gctatggggt
     4081 ggatataggt atcctcattg atatgtacca gcagggggcc aggattgctg aagtgaatat
     4141 cggacatatt gaaaatagaa tgcagtcgct ggaacaattg ggtaaaatgt cccgtgaagt
     4201 ggccgctgtg atacttaaga aatccagtca taagggagag aataattatg agacactcga
     4261 acatatacaa ctgatccggg agcaaatgga gtttgccatc cgggaaagtt cgaggagttt
     4321 gaagaaagtg gccatttttg atatggataa tacgctgcta cgggccagtt ttattcatac
     4381 agcagcggaa cattttgggt tcaggaaaga actgattgaa attgttacta cacaaaacaa
     4441 tccttttatc cggactaaac tgattgcccg tttattaaaa gggcgggatt tcagcgagct
     4501 gatccggctg gctgattcaa ttcccgttac cgatcatacc agtgaaatca ttcagctgct
     4561 ccgggataat ggatttgtgg tgggaattat atctgacagt tatgattgta tcaccaatca
     4621 tttgaaaaac cggtttggct ttgatttttc cctggccaat gaactggaat tttcaaaaag
     4681 cgtcgctacc ggtgaggtta aaataccttc cttttttctg tcacatgaag gcagtatgtg
     4741 taatcatgag tattgcaaaa cacatgcact gacccatatt tgcaaccggt tttcagtacc
     4801 tgtttcagat acactggcca ttggcgacag tgaaaatgat atctgtatta taaaaaaagc
     4861 cggaatcggg gtttcttttt gttcatccaa tcagttgctg gatgctgtag ctgattttat
     4921 tgttaaagaa gctgatttta atttattaac tccagtaatt gaataaactg tatgttcatt
     4981 tttttcctga tcgctgtttt tgtcattggt tatattgcca ttgcctttga acatccgttg
     5041 cggctgaata aagccgcttc ggccatgatc accggtgttc tttgctggac gatttatatg
     5101 ttcagttctg catccgcaga aacagtaaca gacgaactgc ttcatcacct gggagagatc
     5161 gcctccattc ttttctttct gctgggggcc atgactattg tggaactgat cgatacacat
     5221 aatggctttg agtttattac ccggcatatt acaacccgga gtaaaagccg gctattgatt
     5281 attgtagtgg tgattacttt tttcctgtct gccttactgg ataacctgac caccgcaatt
     5341 gtaatggctt ccctctgtgc ccgcctgtta aagaataaag aagataaact ctggttttgc
     5401 ggtatgattg ttatttcagc caatgccggc ggggcctggt cgccactggg tgacgttacc
     5461 accaccatgc tctggatcgg tggtcagatt accgcgttaa atattatgaa acaactgatt
     5521 ctgcccagcc tggtcgtttg tgcattgccc ttggcactgg tagcctggcg gttcagggga
     5581 cagcagattg accggttgga agaaaaggat gatcaaacaa aagccgaaaa gaaagatggg
     5641 ttgctgatcc ttttttccgg aatcggtttc ctggtttttg tacccgtatt taaaacgctg
     5701 acccatttac ctccatttat gggtatgctg ctggcactag gcctgatgtg ggtgattacc
     5761 acttttctac ataaagacaa agaacctgag cagctggaaa aacttaccgt agcccgggca
     5821 cttcagaaag tggatagtcc cagtatcctg tttttcctgg gtatactgct ggctgtttct
     5881 tccctgcaat cagccggcct gcttcagaca ggagccagtt ggttaagcaa tacactcggg
     5941 aatgattata tgatcggttc tttcctggga ttattgtcgg ccgtggtgga taatgtaccg
     6001 ctggttgcag catcccaggg tatgtatgat ctgcagacct atccgaccga tcatcctttc
     6061 tgggaattcc tggcgcttac taccggaacc ggtggcagtg ccattattat aggttccgcc
     6121 gctggggtgg ctgttatggg gatagaaaaa atcgatttta tgtggtatct ccggaatatc
     6181 agctggctgg ccctgattgg gtttgccggg ggtattgcgg ttttcctgct gcaaatgcaa
     6241 ttttaatatg ggtctgttta gggaaactta ccaacagatt tttatccgga tacggacaaa
     6301 actcagccgg cctcagctga tcatgctggt tgcggtactg acgggtcttg cgtcagggat
     6361 cctggccgta ttactgaaac ttcttgttca ctggattcaa cgcggaatca gccaggtcaa
     6421 tacccattca tatacgcact tgttttttcc tgcggccggc cttctgttaa ctgtattgat
     6481 aatacgatat atgttcaggg gatttattga aaggggtatt gcaatggtgc tccgttccat
     6541 tgcccggcgg agttctttta ttccgctaag tcatacctac ctgcatgtca ttaccagctc
     6601 agtaactgtt ggttttggag ggtctgccgg tctggaggca ccgattgttg ccacgggttc
     6661 tgctgcaggt tccaccatcg ggcgtatcag cgatcttacc taccaggaac ggaccctgct
     6721 gattgcctgc ggagccgcag ctggcattgc ggctgttttc aatgcaccga ttgccggagt
     6781 gatttttgcc gtggaaattc tccttaccga aatgctggtc agtcatttta tccctttgat
     6841 aatatcttct gttacgggtg tgctgtgttc caaagtgatc ctttccgaag cttcactttt
     6901 taacttcaga ctgacccagg attttgatta caggaatgtt ccttattatc ttttactggg
     6961 cattttggct ggctttattt ccttgtatta tgcccgggca tttaaaagca cggaaagccg
     7021 cctgcatcac tggaagctgc cacctttgat gaaagcactc acaggtggca tactgctggc
     7081 cggtttgtat tttatattgc ccccgttgtt tggagaaggg tatgaaagtg tgaaagtagt
     7141 tgccagcggg acttttaatt cccttacggg tacctcaagc cctttgtcac agcttggcga
     7201 cacagccggg atcattgttt tcgctgccat gattgtattg ctgaaaccgg tagccgctgc
     7261 catcaccatt ggtagcggtg gaaacggtgg aaattttgcc ccttcccttt ttaccggctc
     7321 ttacctgggt ttcttttttt ccaagttgct caatgccact ccctggatca aaatacccgt
     7381 gggcaatttc agcctggtag ggatggccgg tgtgctgagt ggggtgatgt attcgccact
     7441 gacagccatc ttcctgattg ccgagatcac caacggttat gaactgttca tcccgctgat
     7501 gattgtgtcg tctatctctt tttttatcgt taaatcctat gagccctatt cgatggagct
     7561 taagaagttg gctatggagg gcgaagtatt cacacataag aaagagaaaa atatactcac
     7621 cagttttcag ctcagtgata tcctgcatga taattttgaa accatttcag tagataaaaa
     7681 gctaggggac ctggtggaga taatcaaaaa aagtgaaaag aatatttacg cggtagtgga
     7741 tagtaaagga aaatttgccg gcattattga actaaacgac atcaaacaac gtctctttga
     7801 ttccagccag tttgatattg tacccgtcag gacccttatg aaaaagcccc cggcggtttt
     7861 acaggaagat gaacatatgc attccgtcat ggaaaaattt gacctgaccc ggagttggtt
     7921 tcttccggtt ttaaataagg ataaaatatt tgttggcttt atttcaaaaa caaaaatatt
     7981 ctataagtac cgtgagattc tgtctgccag tgccgatatt tatgaacagg aaagcggtaa
     8041 caaaccaggc tgatttgttt atccttcttt ctttttcagc agttcttcaa tccttgaagc
     8101 cagtgagtcc gggctttcac ccggtattct ttccattccc tgcagcgcat ccacaaaggt
     8161 ctgaatggcc cgttcataag cggcaaactg gtttttcagc acattcaggt cattggattc
     8221 ccagtatccg ccctcgtcag tcatttcaaa atggctgaag tattttttac tgagataacg
     8281 gaagagccgg atcagggcca catgggcatc gataccggca tactgggtct tggtactgat
     8341 ggtatagatc agttccgggt ccagcccgtg ttccaccagc atttcccggg ttatatgact
     8401 gataggagaa agcagtcgtc cctccggggt aaaagtgagg aacagaggct cacattcctc
     8461 cggggcaaaa tagatccctt ccaacggatc cttttcatcg gcttcaaaat agtgatagtc
     8521 ccagcccagg ctttcacaga tatctttcat ttccgcgccc agctgcggca cgagggagcg
     8581 gtctttgatg ctgccggagt agtgaatgga gagtcccata atcagttttt ttaattggca
     8641 taaaattctc caatagatcg caggctgcca atggaaaaac aacagtgatt tcgttttttt
     8701 tccgacgcct gccgtttaaa ataccaaaaa agcggcaaat ccattcatgc cttatttacc
     8761 ggtagactgt ttcagcgact ggttctaccc cgtttctgaa cgatcctgac ttacagaaac
     8821 gtataaactt cctgctgacc tgtataactg ttgaggaagt ttattggatc atctttactg
     8881 cagacccgga tcaatggagc cgggtgagtg gacaggttga tatgaaacaa tagaaatgaa
     8941 gaagtatttt tttatcataa aatcactggt cgccgggctg atcgtgttat tcgtgctgga
     9001 gggatgtggt acagagtacc attattacca gcaacataaa tcacatagcc caaagtatat
     9061 gaagcgccag gtaaagtata aaaagtatca gcatcatgat aatggagtgg aaataaaaat
     9121 ccgtcattag ccgcaggtgc tgtggttgaa taagcaagga aaatgctgac tgaagcgaat
     9181 tcatctggta caggaagcat tgaacaggca ttttaagcaa agggtggcag gaagaacagc
     9241 cgcccttttt ttatctttat ggtctaaatt tcagacccga tgaaaagaag ctgctggatt
     9301 gtacttttta ttttaatgta ttccgcttta gctgcccaaa ctgataccat aaaaaaattc
     9361 cccaatggta atatattatt gagaaaggtg gcaggaatgc agctgctttc gccgtctggc
     9421 cagaatctgg gcagctggac agaaaccatg cataataata agcagggagg ccggtttgta
     9481 agtattgtca gtaagacgga ttcgcttgga aaaagaaaat caggtgtatt tgatcagcag
     9541 aatggtgaat tcgttttgcc agtggagttt gaccagctta gcccgactga ttatggaaaa
     9601 ggtttttata ccacacgtat tggaaaaaaa tatggatttt atagtgttcc tgccggaaaa
     9661 cgactgcctc cgatatatga ttttacaggc agacatgtta atcctgcctg gtttatcgta
     9721 gccgtatctg gtgagtataa ttgtggttat gtgtatgatg aaaaattagc cctggtggat
     9781 agcataccgg gtatgaagtc ggttaatagc cggatcgttt cgggtaaccg cgaatggtat
     9841 cagatcaata tggccgatgg ccaggggctg ttggacaaag aaaataaact gctataccgc
     9901 aacgagtgga aacatattca tgatgttaag ggaatgaccg ccattgtcaa taatggaaag
     9961 gcaatcggac tttttgacct cgcaacaggc aaactggtaa agccctacag ctttacggac
    10021 tactggccgg atattttcgg tagtcagggt tttctgaaga atggaaatca atgggtactg
    10081 attgatcagc tggggaagca gttgtttact tttaaagccg aagaagtgga attttcacat
    10141 cggggagaaa ccggtggctt ttactttaaa cagaagggct tgtggggggt gatgaataat
    10201 aaaggcaaaa tagttcgtag cccggtatgg gaatcggtca gtccgggttt agcaggtgga
    10261 tttaccggga aatatcccgg gaagcccgac acttattata acggggttta taaagaaatt
    10321 aatggggaga aagtcctgac aggtattcgt cagctaaaca ccgaagaagt aacagctgcc
    10381 tatccggatc aggccgtttg ggtacgggaa gaacccaaag aagtgattga aatgatgaat
    10441 atacctgctc cggtccggca cgaagaaaac gaggaccata tttttgtaaa aatggaagtg
    10501 gatccggcgt tcaccaaaac aggtgaggtg gagaagacgg tgctccgaca gctggtggat
    10561 gcttataaaa aagaattcca gataaaaaca actggaaccg tgcagctgaa attggtggtg
    10621 gaaaaagatg gaaaaatcag cagtaccatc gtcgtttctt cgcctgatcc cctgctcaca
    10681 gaagcgtcta agaaattgct cggcaatatt accggctgga aacccggtat acagaatgga
    10741 cggaatgtac gcggggagaa gaccctggtg ctggaatggt aacaaatgcc tttatttcac
    10801 cactgctatt acttcccgta taatctgaat tgcattgctc agttcagctt tcgtgctatt
    10861 cccaaatccg atcctgatgc cattgctttc aggaggatag gtatataaat catccctttc
    10921 aggcaaaagc agtccttttt tcatacataa ccggtgaata tcgctgactg gaaactccct
    10981 gttaaactca atccagaccg ccatcccacc ttcaggtgca tggtaagtgc aacacccggc
    11041 cagattttta tccagtaaac gcagcagaaa atctctttta tgcatatagt attttactgc
    11101 tttgttccgg taacggcgga tgaccgcttc ttttaatgtc agcgccaggg cttcttccag
    11161 taagctatcc gaaaatttat ctatagagac tttcagtttg gccagttcgg ataaaaaatc
    11221 ttctggtccc gcaatatatg atatgcgaag agtcggcgat atcaggcggt tcaatccgcc
    11281 aatataaacc acccttttct ggttatcata agcggccacc gggaaccccg gtgcattcgt
    11341 ataatgaaaa tcgaaatcgt aatcgtcctc aaggatcatg aaattgtatt gttctgccag
    11401 ttgcagcaat tgtttccttc tttcttccga tagttttaca caggtaggat aatggatatg
    11461 aggagtgata taaactgctt ttattttttt gcgtttacag atttttccga gttcaccaag
    11521 actgattcca tcctgatcag tcttaacggt ttcaatgttc aggttcaggt accggcaaat
    11581 actatacacg ccggaataat tatgctcagc aaatacaatg ctgtcctgtt ctgtcattgt
    11641 aagtgaaagg accaggaata tactgaactg gttacccttt gatataagca ggtttgacgt
    11701 gccggtattc aggccccgcg tgtcgttcag gaaccgggaa agtgcttttc tcaattcaaa
    11761 ggacccccac gcattttctt tattaaaaag acggctatgt tcttttttcc ggatgagcag
    11821 gcgatggttt ttctccagtt ccacggcggg catatagcca atatccggct gcccgtcatt
    11881 caggatgatg tcatagattt ttttcccctc cttgctatac cctgtttggc ggggaggaag
    11941 tccgatcttc ctgaatggga acgtagcctt atgttcagct gctgacaata cttccttttt
    12001 gcctgtagcg gagagaacgg gaggtttatc tgtaatatac gtaccggaat ggggttttac
    12061 cgttacccag ccttgtgcat caagctcttc atacacctgc tcgatggtcc ggctgtgcaa
    12121 tccggtttgt tgtgccattt ttttaacacc gggcagtttt aatccccctg gtaaaatgcc
    12181 attcctgatg gcatcggaca ggcattctgc tacctgcagg tagatcggtt cgggtttatt
    12241 cttattgatt tttatcagcg atgggatatg cataaactga cctaattaaa aatataaatc
    12301 tgacccttcc cgttacatca attagttgca attttggtct atgaagatcg gatttgtgat
    12361 ggtggtttgc gggatcgcaa tcctctcagg ctcatttaaa attaataaaa ttatgaatga
    12421 aaataacgga accggagcgg tgcaattcag aattgcccgg ccaacagata aactggaaga
    12481 ggtggttgat ttttacaaaa cggtgctggg tctggaagag atcggggcat ttaaacagca
    12541 tgaaggatat gatggggtga tgctgggtct tcctggtttt gattatcacc tggaatttac
    12601 ccaacacacg gatggcagtc cttgtccggc gccaacaaag gataacctgc tggtgctgta
    12661 ttttgacacg cctttgaagt ataaccgtgc ggttgaacgg gccttacaat tgggtcacca
    12721 gccggttgaa ccggagaata gttactggaa ggggaaaagt gaaacttttg aagatcccga
    12781 tggatggaga gtagtatttt ttaatggtgt atataaaccg tgaatgctat gcgtatacaa
    12841 aaactggtac tacaaaccac ggatctgcaa agacagtatt cgttttatat tgaaaaattc
    12901 aacttccagg ttgtaacagc ggaccaggac cattttagtt tcagtgccgg cgcttcgctc
    12961 gtggagttca gggcttattc aggcaaggat attttcccat atcacctggc gttcaccata
    13021 cctgcaggcc agctgatcaa agcagcggat tatctggctg ctaaaggcat aaacctggtg
    13081 aaaatgggga atcagactat tttccaattt gagagctgga atgccacatc cttatatttc
    13141 agggacccgg atgaaaatat acttgaactg atcgttcgtt ttaacctggg gctaaagaac
    13201 ggctccgtac atttttcccc ctcagatata attcaggtca gtgaaatagg aattgtggcc
    13261 gacgatccgc aggctttttc cagttatatc actgaacagt ccagcctgcc cgtctggaaa
    13321 atggccggca atgactttat tgcaatgggc gatgaagagg ggttactaat tgttgtacag
    13381 aagaacagaa attggtatcc aacggatact ccggcaagag ccatgcctgt ctatatacat
    13441 tcgcccgctt tcaaccggag catacaggct gatccctacc tgttcgctcc ctgattcatt
    13501 accaataagc tgaatcgaac tggcaggaca tgaaaatgtg ctgaatggcg gagctgtatg
    13561 caaacgataa caaaaacctg actgtaacca ctcagtccaa taatttaaaa tcaaaagaca
    13621 acaaaatgaa aaaaacaatt ttcctgctgc tactgaccgt attttcagta agtggccaag
    13681 cgcaaaaacg tcaaccttct gtggcaggct atggggctgc tgtagcagaa ctgaccagca
    13741 tcgacaaaaa agctgccctg aacctgggag gatatggggg tgtattgatc aatcatcgtt
    13801 tcctgctcgg ggcctccggg aataatattt tattctcaaa aaccatcaat ggttctaaga
    13861 cggattttca gttcaattat tacggactga tcacggagta taaattcttt ccggggagtt
    13921 tcgtgcattt ctcagccgga ctgacatccg cgcttggatg gctggagaat gatatcctga
    13981 ccgctgacaa aaaaaagcgc atggatggcg attatgtata tgtgatccaa ccccgtctgg
    14041 cactggaact tcgtatcact tcatttatgc aggggaaatt gtatggcaac taccgcttta
    14101 ccggaaatac aaagagccgg tacttcacgc cccggaattt agatggagcc ggtgccggtg
    14161 cggcactggt ttttggctct ttctagtggt ggtaaaacgt catcaaaata taccggcggg
    14221 gataaccgat catcaaccgg gatcagttac cccggatttt aattcattta tctacaatca
    14281 gggattatgg aaaaaagcat tcaaacagaa attattattc atgcctcccg tgaaaaagta
    14341 tgggaagtat taacggactt cagcagctat gcgttatgga atccatttat ggtttcggca
    14401 tccggcaaat tagctaaagg gcaaaggttg aaaattcaac ttcagaacgg cagcagcacc
    14461 atgactttca gtcctgctgt gcgttctgtt atccccggct tttcattttc gtgggagggg
    14521 cgtttgtttt tcaaaggact ttttgacggg tatcattttt ttaaaatcat acaaaacagc
    14581 agtaatcagg taaaactggt ccagggagaa cggttttcag gaatattcag ccggatgata
    14641 tttcataaga tcggtgccag tacggcggtg aattttgatc ggatgaacag ggaattaaag
    14701 aagcgggctg aaatgaaaga gataagagcc actaatcaca taaagattac cggtgatgca
    14761 taagcaacga atgcgctata tggataatct gaaagttatg cttacctgcc tggtagtagc
    14821 acatcatgcc gggcaggcgt atgggccatc gggcggagtc tgggtggtga aagaagcaga
    14881 tgcccttccc tggctcagga tttttttcta tgtgaacgca tcgtttatga tgggtttgta
    14941 tttttttatt tccggatatt ttatggtggt cagtcttgaa aagaaaacaa ccggccggtt
    15001 tataaaagac cggctgaaac gacttggtat cccattattg ttcttcacat tcctggtttt
    15061 tcttcctttt aactatgtag tttccggtgg agaagggaat attttctttg tcctggctga
    15121 tacttattta caccagcctc ctttagctac tggacattta tggtttatcg cttcattgct
    15181 tctatatact tttctttacc tggcaataag aagaatggtc cgtccggtgc cagcccgcgg
    15241 gtttattttc agaccatatt acatcgcggt atatatcttt ttactcggtt tggtgactgt
    15301 gtttgtacgc tcgtattatc ccattgatca gtggagaacc tggctgatac cgctggaaat
    15361 agcgcatgtg ccacaatacc tgagcctgtt tttgctggga attttattta atcagttttg
    15421 ctggctcgaa caacttaaac tccggtatgg gtttctggta ttgattataa cagccgccat
    15481 tccagtttta cccggaaccg atttgttgga taccacatgg atagcatcgt tcgaagaatc
    15541 cggactttgt gtgggtttgt ccattatcct gctggtgatt tttcggaatt attataacca
    15601 ggagtcggca ataactctat ccttttcaca gaactcttac ggtatttatc tttttcattt
    15661 gttgattgta attgcattac aacagttgct cgtaaattgg ccggcagctg cagaattcaa
    15721 atttcccctg gtggttgtat tgggggtgtt cgtttcattc atgctttctc gactcctgag
    15781 gaagataccc ctggtcagac gtataattta atgtatgagg cttgttggct gaatctacat
    15841 gggctctgtt taatcaggaa tgatttcagc agtttcgggt aattggtttt tcgaacagtg
    15901 ggggactgtt tttagtacac aaaaaggggt atatttagct atcaacccgt catttcgggt
    15961 gtcattgaat caaaccctca aaggccctgc ttatgatttg tcgttcaggt ttattattgt
    16021 tgttctccct gcattgttcc ctgattttac cggcacagcc gcttagtaca gcggatcaga
    16081 tccctgcaaa ctccttcaac ccttccactt tattatggta cgatcagccc gcccggaaat
    16141 gggaggaggc cctgcccgtg ggcaatggcc ggctgggggc catggtgttt gggaagaata
    16201 aggaagagcg gattcaatta aatgaagaga cctattggtc gggcggacct tattccactg
    16261 tagtcaaagg cgggcataat tacctgaaag aaattcaaca gaaagttttt gacggtaaat
    16321 tcctggaagc acataatctc tttggccggg aactgatggg ctacccggtg gaacaacaga
    16381 aataccagtc gctcggcaac cttcatttgt ttttcaacaa ccaggatagt gtaaccgcct
    16441 ataagcgctg gctggatctg gcaacaggta tagcgggggt tagttatgaa gcagctggtg
    16501 tacgctacca acgcgagaca tttgcttccg ctcccggaca ggtgatcgtg gttcgtctta
    16561 cggccagcaa acccggcagt atttccttta ctgcgaatct gcggggtgaa cgcaaccagg
    16621 cccattccaa ttatgccacc gattatttcc ggatggatcc ttacggacag gatggattga
    16681 tcctcacagg caagtcggct gattacatgg gtgtggaagg caagctccgt tatgaagccc
    16741 ggatcaaagc ggtgaatgaa gggggtacga ttactaccaa tggagtggat cttaatgtca
    16801 gcaaggctga tgccgtgacc ttgtattttg cagcggctac caattttgtc aactacaaag
    16861 atgtaagcgg tgatccgcac aaacgggtgg aagactgttt taataaactg ggcaatcaat
    16921 catttacttc tatcctgaat gaggcggtta ttgatcacag tagttatttc aaccgggtgt
    16981 cattgaaact gccggtcaac tcccagtctt ttttgccggt aacagaaaga ataaagcgga
    17041 tacaatccgc tcccgatcct gcaatggccg ccctgagtta tcagtttggc cgttacctga
    17101 tgatcgcttc ttcccgtccg ggtacacaac ccgccaacct gcagggtatc tggaatgata
    17161 atatgaaccc gtcctgggat tcgaagtaca cgactaatat caatacggaa atgaattact
    17221 ggccggtgga aagcggcaat ctttctgaat gcgccgaac
//
LOCUS       DAOPMW010000029        17111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3240_length_17111_cov_7.997772, whole genome shotgun sequence.
ACCESSION   DAOPMW010000029 DAOPMW010000000
VERSION     DAOPMW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17111
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_17111_cov_7.997772"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLU11_02835"
     CDS             complement(<1..682)
                     /locus_tag="PLU11_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPN58074.1"
                     /translation="MHKQTSRREALKKLAAGSLAAGSLTSLSSFTDQAMEEQQSLKGN
                     INHSVCSWTFNFMPLDELCGTVKKLGFSAIDLLTPKDWPTVQQHGITCSMCYTAGKIS
                     LTEGWNNKNNHAWLIKDFNEAIPLVAKAGYKNLICFSGNRNGMDDETGMKNCAEGLKQ
                     IIGLAEKEGVIIQMELFNSKVDHKDYMADKSAWGVNLCKMIGSPNFKLLYDIYHMQIN
                     EGDVIRTIR"
     gene            complement(699..1922)
                     /locus_tag="PLU11_02840"
     CDS             complement(699..1922)
                     /locus_tag="PLU11_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPN58075.1"
                     /translation="MKSTTRIQLSVMMFLEFFIWGGWFVTLGTFLGTNLQADGASIGQ
                     VFSTQSWGAIIAPFIIGLIADRFFNAEKILGVLHIAGAILMYQMYTADNISTFYPYVF
                     GYMVLYMPTLALVNSVSFNQMKNPEKDFSSIRVWGTIGWIVAGLLISYLFHWDSKENI
                     AAGMLKNTFLLSGSAALVLGLLSFTLPKTPPSKKSGEKVSVGDIIGLDALKLLKDKNF
                     AIFFLASVAICIPLAFYYQNTNPFLTNIGMSNPTGKMTIGQGSEVLFLLALPVFFTRF
                     GFKKTILVGMLAWALRYVLFAYGNAGELGFMLITGIALHGICYDFFFVSGQIYTNSKA
                     GEQYKSAAQGLITLATYGVGMLIGFKVAGMITDAYKTADGFDYKMIWLIPAAIAAAVM
                     LLFALSFKDEKVTKS"
     gene            2074..3234
                     /locus_tag="PLU11_02845"
     CDS             2074..3234
                     /locus_tag="PLU11_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN58076.1"
                     /translation="MQNRKLRMAMIGGGIDAFIGAVHRIALNMDGQVELVAGALSVNP
                     EVALESGRQLFLSEDRIYTDYKTMLEKEAAMPADKRIDFVTIVTPNFVHFDPAMMALD
                     KGFNVVIEKPITFSLEEAKLLKAKLEATGLSLLLCHTYNGYPMVKQAKRMLQQGVLGK
                     IRKVYVEYPQGWLSTFLEKTGQTQAAWRTDPKKSGKAGSMGDIGTHAFNLAEYVTGLK
                     VTSLCSNVNIIVEGRMLDDDGAAFLKFDNGATGVLMATQIAAGEENNVKIRVYGEKGG
                     LEWKHEDTNTLLVKWLDKPVEIYRAGTGYLDSFASHNCRTPAGHPEGFLEAFANLYRN
                     FVLTVRAKINGETPKPEWLDFPGVEEGIRGMAFIENIIESGKSDTKWTPFVV"
     gene            3264..4319
                     /locus_tag="PLU11_02850"
     CDS             3264..4319
                     /locus_tag="PLU11_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPN58077.1"
                     /translation="MTTIKGPGIFLAQFMGNEAPFNNLKSICLWAESLGFKAIQIPTW
                     DDRCIDLKLAAESKTYADEIKGIVNDCGLEIAELSTHLQGQLVAVHPAYDTLFDGFAP
                     EALRGNPKARMEWAIQQVKYGAKASQNLGLTAHPTFSGSLLWHTFHPWPQRPAGLVEE
                     GFKELGKRWTPILNYFDECGVDVCYEIHPGEDLFDGATYEMFLEEVNNHPRACLLYDP
                     SHFVLQQLDYIQYIDFYHERIRAFHVKDAEFNPTGRQGTFGGYQSWANRAGRYRSPGD
                     GQVDFKTIFSKLTQYDYPGWAVMEWECCIKHPEDGAREGAEFISKHIIRVTEKAFDDF
                     AASGTDEKANRLLLGLD"
     gene            4366..4743
                     /locus_tag="PLU11_02855"
     CDS             4366..4743
                     /locus_tag="PLU11_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPN58078.1"
                     /translation="MKKMILPLFAIVMLASCGGADTSGGKTTEPVAEKPKDPAYDKGL
                     ALVAQNDCLTCHKINEPFTGPSYSQVAEKYAGADAAKIKEISEKIIKGGTGVWGSALM
                     TPHASLSQEDAEAMVKYILLLKK"
     gene            4750..5526
                     /locus_tag="PLU11_02860"
     CDS             4750..5526
                     /locus_tag="PLU11_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN58079.1"
                     /translation="MKTLLIGTIVALSAVSCNNSSETTKNAEGDSLVPPTTAMANVLT
                     EEQQKEGWQLLFDGQTTKGWHKYGGSPVGSAWKVADGMLYLDTTDKKDWQIRDGGDIV
                     SDSAYDNFHLKLEWKIAKDGNSGIIFYIHEDTAKYEWPWNTGPEMKVLDNSGHPDSKI
                     IKHRAGDLYDLISCSKETVKPAGEWNLAEIKSQNAKLELSLNGEVVVSTTLWDDAWKK
                     LVAGSKFSKMPGFGTYQKGKIGLQDHGNMVWYRNVMIRKL"
     gene            complement(5577..7067)
                     /gene="pyk"
                     /locus_tag="PLU11_02865"
     CDS             complement(5577..7067)
                     /gene="pyk"
                     /locus_tag="PLU11_02865"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPN58080.1"
                     /translation="MNKNIDKYLHKSFDKEAGRIHAFKRTKIVATVGPACDTYEKLLE
                     LVKAGVNVFRLNFSHGAHEDKARIIELIHKINKSEPYNIAILADLQGPKLRVGDLEGG
                     ALEINEGDILTFTNEKVIGNRERIYVSYPDLHTDVKVGEKILFDDGKLEVIVKEITAA
                     KEVKVEVTLGGVLLPKKGMNLPDTRISLPSLTEKDLADLEFIMKQRVEWIALSFVRSP
                     DDITGLKEKIKKAGHSAKVIAKIEMPQAIVNLRDIIVESDGIMIARGDLGVELPVEAI
                     PLIQKDIIKKCLHRAKPVIVATQMMESMIERTKPNRSEITDVANAVLEGADAVMLSGE
                     TATGKYPKLVVETMTKIIMEVETKAYDYNREEMLKPQSHSPSFLSDAICYNACKLAED
                     VNADALIGMTQSGYTGFMLSSYRPKAHLYIFTKIRSLVNQLSLSWGVRAFYYDEEESL
                     DDIFSDQIDILKERDFVRSGDVVVSTGSTPIHLHLPTNVLKIAKVE"
     gene            complement(7113..8096)
                     /gene="pfkA"
                     /locus_tag="PLU11_02870"
     CDS             complement(7113..8096)
                     /gene="pfkA"
                     /locus_tag="PLU11_02870"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPN58081.1"
                     /translation="MEKKVTRIGVLTSGGDAPGMNAAIRAVVRTGIYHGLEVFGIMRG
                     YQGLIDDDIFRMEGRSVANIIQRGGTILKTARCKEFYEPAGRKTGYENLKKRGINGLV
                     IIGGDGSFRGAVNFSKEFDIPCVGLAGTIDKDISGTDFTIGFDTAVNTAVEAIDKVRD
                     TMDAHDRIFIIEVMGRDAGYIALHSGIATGAENILIPERKTDINGIISSLQEKERRKK
                     MVNLIVVAEGDEFGGANEVQRVLQEKLPKAEIRVCILGHIQRGGAPSCMDRLIASRMG
                     YHAVECLLEGRHNVFVGIQNNKMNYIPLETAVKNKGKISDEWMKIVKILAS"
     gene            8389..10527
                     /locus_tag="PLU11_02875"
     CDS             8389..10527
                     /locus_tag="PLU11_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN58082.1"
                     /translation="MKKLLLLLGILPVLASGQIKYPVTAKGTTVDNYHGTTVPDPYRW
                     LEDDRSDSTRDWVTRQNKVTFDYLGKIQYRKQWQDRLEDINNYAKYSSPFRQGTNFYF
                     YKNDGLQNQSVLYRQKGLNGKPELLLDPNQFSAEGTTSLAAFSISKNGKYAVVGKSAG
                     GSDWRTFFVMDLTTKKYLKDSLAWVKVSGAAWKGNGFFYSRYPAPEKGKELSTKNENH
                     QVFYHVVGTSQDKDQLVYEDKANPQQFNSVFTDESERYVFLTSNDRGKGKEGNSLWYY
                     DSKSTDKKFKPLIKDIGDFQYSFIEEVNGKFLLQTNEGARNRRIIQVDPLHPEKENWT
                     TFIPEVEENMSSVTSAGSKLFVTYMKDVTSRVYVYDLGGKLEREVKLPGLGTVSGFGG
                     EREDPFVFYTFTSITYPPTIFKYDIATGESSLFRKPEVKFDPEDYVTEQVFYPSKDGT
                     QIPMFIVHKKGLELNGKNPAILYGYGGFNISSTPNFSSARIAWLEQGGVYCIANLRGG
                     SEYGEKWHEAGMREKKQNVFDDFITAAEFLIAKGYTSPDYLAAQGGSNGGLLVGTVIN
                     QRPELFKVAIPQVGVMDMLRFHKFTIGWNWIAEYGSSDKPGDFKNLYSYSPIHNIKPG
                     INYPATLVTTADHDDRVVPAHSFKYAATLQEFYQGNNPLLIRIDVNSGHGASNLKKSL
                     ETTADIYSFIFYNMGLKPEFKLTADGKKAF"
     gene            10550..11440
                     /locus_tag="PLU11_02880"
     CDS             10550..11440
                     /locus_tag="PLU11_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN58083.1"
                     /translation="MKVLITGANGFLGHYLVTALLQQGFNVLATGKGACRLAGQGTNG
                     FDYAEMDFTDLSSTRDTVSRYAPDIIVHAGAVSKPDDCELDQALAWRINTGGTHHLLE
                     AAAAHQAFFIFISTDFVFDGEKGMYAEEDQTVPVNYYGQTKLEAEKAVHQYPHPWAVV
                     RTVLVYGEPLAGRSNILSIVKDKLEKGEGYKVVDDQVRTPTYVGDLAEGIVSIIQKKA
                     TGTWHLSGAEVLTPYEMACKAADFLGLDKGLLTRVTADTFTQPAKRPAKTGFNISKAK
                     MVLGFKPVSFAEGLAKTFLG"
     gene            complement(11474..12064)
                     /locus_tag="PLU11_02885"
     CDS             complement(11474..12064)
                     /locus_tag="PLU11_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPN58084.1"
                     /translation="MKYSLLFVLLFSGHLIVAQPPQYLDSVSAFRNQYINTHGVIKEK
                     DRAGLQFYPIDTSFLMPSRVERIYEAPWFAMETSGKIKKTFRVYALLHFTMSGKPLRL
                     TVYQSQQLMQSEEYKDYLFIPFTDLSNGEQTYENGRYIDIRTGDLENSPFLLDFNKAY
                     NPYCAYVSNVYNCPVPPVENKLAVPVNAGEKKFSLH"
     gene            complement(12073..13020)
                     /locus_tag="PLU11_02890"
     CDS             complement(12073..13020)
                     /locus_tag="PLU11_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HPN58085.1"
                     /translation="MKNIKLIEVPSEIGAGTRGASLGIEAIKIAALDFMSNFFIHFPS
                     EKIQTENKLLYEPIESPYAKRIKGVSSIYERVSKSVTETIKNNFFPVVLSGDHSTAGA
                     VIAGIKMAKPKCKLGVIWIDAHADLHTPYTTPSGNMHGMPMAVSINEDNEECAVHTLD
                     EETTRQWNQLKALGKITPKVLPENVVFISLRDFEKEERHLIEKYGMKVITTKEVRNKG
                     AENVVRSAIRYLSDCTDIFVSFDVDSLDSSISKGTGTPVGNGLREREAEDLISKFMQN
                     RKICCFEIAEVNPTLDKENLMAEIAFNILQRSVNVLMMN"
     gene            complement(13275..14060)
                     /locus_tag="PLU11_02895"
     CDS             complement(13275..14060)
                     /locus_tag="PLU11_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN58086.1"
                     /translation="MHRIIIPVDFSETSLNAARYASKMLAWNESATAVLYHNYENEDD
                     LDISLYYLESLKKEFNKAGVRQVECEHELGGDLIENISRLAHTMRATLVMMGITGKSA
                     IRQVMFGSNTLKLIDKNLYPVMIIPPDAVYKGVTKVAFATDYQHVELSTPSQLIGSVL
                     KLLDPKLHIVHVSKEAIKTLPAALQLEKEKVDQMFADFKKEFFFITNEDFYEALDSFV
                     KEQDIDILVTVPKHPSNSSSLFRTSHTKKLAYHSHIPILAAHQ"
     gene            14187..14648
                     /locus_tag="PLU11_02900"
     CDS             14187..14648
                     /locus_tag="PLU11_02900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN58087.1"
                     /translation="MNQPKIREALVSDISQIQRVRNAVKENTLSDPALVPDSDVADYI
                     TRRGKGWVSLEGEAVTGFAIVSVSDQNVWALFIEPGFDKLGIGRQLHDRMMDWYFEQT
                     QETIWLSTTPGTRAEGFYRKAGWEEAGEYGKGEVKFIMTPENWSKQKKLAG"
     gene            14736..15851
                     /locus_tag="PLU11_02905"
     CDS             14736..15851
                     /locus_tag="PLU11_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN58088.1"
                     /translation="MRRIGYFFSLFPLIFFAFWVKQPPAQSDLLSKADGPYLIYRKDT
                     LFVHTIYDAGGVTKLRTDTLKTGAKKEIQLKVPTGNPDEFFTVTLKSRLQQEKAEYRK
                     APRQLAISDMEGDLKGFLKLLKGNGVIDQQFNWTFGDGHLVLTGDFFDRGVQVTELLW
                     FIYSLEEKAKAAGGYVHFVLGNHEIMNMNGDFRYVQPRYMEHAALMQLDYLSLFDEQS
                     ELGRWLRTKNIVEKVGEVLYMHGGISSEVNLFNAAAPVLNELARPYYGDTSYLYPNVH
                     VEILYSEVGPFWYRGYYIGSPKATEAQIDSTLSIFGARYIATGHTIARDTISTWFNNK
                     LFNTDVPHSKGFSEGLLLEKGKFYRVNEAGDRFLIAG"
     gene            15957..16946
                     /locus_tag="PLU11_02910"
     CDS             15957..16946
                     /locus_tag="PLU11_02910"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HPN58089.1"
                     /translation="MARSIAFREAIREALSEEMRRDERVFLMGEEVAEYNGAYKVSQG
                     MLAEFGAKRVIDTPISELGFTAIGVGAAQNGLRPVVEYMTWNFAVLALDQILNTASKM
                     LAMSGGQISCPIVFRGPNGSAGQLGAQHSTAFEALYANIPGIKVVSPSNGYDAKGLLK
                     QAIRFEEDPVMFMESEQMYGDKSEVPDEEYYIELGKADVKRAGRDVTIVSFNKMMKVA
                     LGAAEELEKEGISAEVIDLRTIRPLDWKTILESVKKTNRLVIVEEQWPFASISSEITY
                     QIQKEGFDYLDAPIRRITAADAPLHYAPNLVAAALPDVPRTVKLVKEVMYQVK"
BASE COUNT         4637 a         3773 c         4145 g         4556 t
ORIGIN      
        1 cccggatcgt ccggatcaca tcgccttcat tgatctgcat gtgatagata tcataaagca
       61 gtttgaagtt aggggaacca atcattttgc agaggttcac cccccaggca ctcttgtcgg
      121 ccatatagtc tttgtgatcc actttgctgt tgaataactc catctggatg atcacgcctt
      181 ccttttcggc cagcccgata atttgtttca acccttccgc acagttcttc attccggtct
      241 cgtcatccat gccattccgg ttaccactga aacagatcag gtttttatac ccggcttttg
      301 caacgagggg gattgcttca ttaaaatctt ttatcagcca ggcatggttg tttttattat
      361 tccagccttc tgtcagacta atcttcccgg ccgtataaca catcgaacag gtgatgccgt
      421 gctgctgaac tgttggccag tctttgggcg tcaacagatc gatcgcggaa aaacccagtt
      481 tttttaccgt cccgcataat tcatccaggg gcatgaaatt aaaggtccat gaacagacgg
      541 aatggttgat attgcctttc agggattgtt gttcctccat agcttgatcg gtaaaagaag
      601 acaatgaagt cagacttccc gctgccaggg atccggcagc cagctttttt aacgcctcac
      661 gtcttgatgt ttgtttatgc ataagcctgt tttgccggtc aggatttggt tactttttca
      721 tctttaaaac tcagggcaaa caataacatg actgccgctg ctatcgctgc cggaatcagc
      781 cagatcattt tataatcaaa accatcggcg gtcttgtacg catccgtgat cataccggcc
      841 actttaaaac cgatcagcat ccccacacca taggtagcga gggtgatcag tccctgggcc
      901 gcactcttat attgttcacc ggcttttgaa ttggtataga tctgaccgga tacaaaaaag
      961 aaatcatagc aaataccatg cagggcgata cccgtgatca gcataaagcc cagttcgccg
     1021 gcattgccat aggcaaacaa aacgtaacgg agtgcccagg ccagcatacc taccaggatg
     1081 gtttttttga agccaaagcg ggtaaagaac acaggcaggg ccagcaggaa caacacttcc
     1141 gaaccctgtc ctatggtcat cttaccggta ggatttgaca taccgatatt ggtcaggaaa
     1201 gggttcgtat tctgatagta aaaggcgagc ggaatacaaa tagcaacaga agcgaggaaa
     1261 aagatggcaa agtttttatc ttttaacagc ttcaatgcat ccagaccaat gatatcgccg
     1321 acgctgactt tctccccgct ttttttggag ggtggggttt taggcaatgt aaaactcagt
     1381 aatcctaaaa ccaatgcggc ggaaccggat aacaagaaag tgtttttcag cataccggcc
     1441 gcaatatttt ctttagaatc ccaatgaaaa agataactga tcagcaaacc agccacgatc
     1501 catccgatcg ttccccaaac acggatactg gaaaaatctt tttccggatt tttcatctga
     1561 ttgaaggaaa cggaatttac taaggccagt gtgggcatat acagcaccat ataaccgaat
     1621 acatagggat aaaaagtact gatattatct gccgtgtaca tctggtacat cagaatcgct
     1681 ccggcaatat gcaatacccc aaggattttt tccgcattga aaaaacgatc ggcgatcagg
     1741 ccaatgataa agggtgcaat aatggctccc caggattgtg tggaaaatac ctgtccgata
     1801 ctggcaccat cggcctgaag gttcgtgcct aaaaaggtac ccagtgtcac aaaccatcct
     1861 ccccagataa agaattcaag aaacatcatg accgacagct ggatgcgtgt agtggatttc
     1921 atgtgagagc ctttaagtga atcgttagtt ttttaaaaag tgatttcgga aaactaaatg
     1981 tactattttt aaccacatat tttatttatc atttatcaaa ttttacccgc agtaatcttt
     2041 gtttattcac attattcatt tcaaacgttt gcgatgcaaa acagaaaact acgaatggcg
     2101 atgatcggtg gaggtatcga cgcttttata ggagcagtac accggattgc gctcaatatg
     2161 gacgggcagg tggaactggt ggccggggcc ctcagtgtca accctgaagt tgcacttgaa
     2221 tctggccggc aactcttcct cagcgaagac cggatctata cggattacaa gaccatgctg
     2281 gagaaagaag cagccatgcc cgctgataaa cgtatcgatt ttgtgaccat tgtgacgccc
     2341 aatttcgtgc attttgatcc ggctatgatg gccctggata agggcttcaa tgttgtcata
     2401 gagaaaccca tcactttttc cctggaagaa gccaaactgc tgaaagcaaa actggaagca
     2461 accggcctca gtttattgct ctgtcatacc tataacgggt accccatggt gaagcaggcc
     2521 aaacgcatgt tacaacaggg tgtattgggc aaaatccgga aagtatatgt tgagtacccg
     2581 cagggatggc tgagtacctt cctggaaaag accggtcaga cacaggcggc ctggcgaacc
     2641 gacccgaaaa aaagtgggaa ggccggttcg atgggcgata tcggtaccca tgcatttaac
     2701 ctggcggaat atgtaaccgg tttgaaagta accagtcttt gctccaatgt aaatattatt
     2761 gtggaaggcc ggatgctgga cgatgatggc gcggcattcc tgaagtttga taatggagcc
     2821 acaggtgttt taatggctac ccagattgcc gccggggagg aaaacaatgt gaagatccgt
     2881 gtatatggcg agaagggcgg actggaatgg aaacatgaag ataccaatac cttactcgtg
     2941 aaatggctgg ataaaccggt ggaaatttac cgcgccggta ctggctacct ggattctttt
     3001 gccagtcata actgccgcac accggccggt cacccggaag gattcctgga agcttttgcc
     3061 aatctctacc gcaattttgt actgaccgta cgggcaaaaa taaatggcga aacgcccaaa
     3121 ccggaatggc ttgattttcc gggtgtggaa gaagggatac ggggcatggc ttttattgaa
     3181 aatattattg aatccggtaa gtccgatacc aagtggaccc catttgtcgt ataatttttt
     3241 tttgaactat ttattttaaa ccgatgacaa ccattaaagg ccccggaata tttctggccc
     3301 agtttatggg aaatgaagcc ccttttaata atttaaaatc catctgcctc tgggcggaat
     3361 ctcttggttt taaagccatc caaataccca cctgggatga ccggtgtatt gaccttaaac
     3421 tggctgcaga gagcaagacc tatgcggatg aaataaaagg gatcgtcaac gattgcggac
     3481 tggagattgc tgaattgtcc acgcatctgc aggggcaatt ggtggcggtc catccggcct
     3541 atgacacttt gtttgatggt tttgccccgg aagccctgcg cggtaatccc aaagcaagaa
     3601 tggaatgggc cattcaacag gtgaagtatg gcgctaaagc ttcacaaaac ctgggactga
     3661 ccgcgcatcc tactttcagt ggttcgttgt tatggcatac atttcatcca tggccgcaac
     3721 gaccagccgg actggtggaa gaaggattta aagaactcgg aaaacgatgg acacccatcc
     3781 tgaattattt tgatgaatgc ggggtggatg tctgctatga aattcacccg ggagaggatc
     3841 tttttgacgg ggctacttat gaaatgtttc tggaagaagt aaataatcat ccgagagcct
     3901 gcttattgta tgacccctca cattttgtat tgcaacaact ggattatatt cagtatatcg
     3961 atttctatca tgagcggatc cgggccttcc atgttaagga tgcggaattt aatccaactg
     4021 gcaggcaggg cacatttggc gggtaccagt cctgggccaa cagggccggc cgttaccgct
     4081 cacccggaga cgggcaggtg gatttcaaaa ccattttcag caaactgact caatacgatt
     4141 atcccggctg ggccgtgatg gagtgggagt gttgtattaa acatccggaa gacggtgcca
     4201 gggagggggc tgaatttatc agtaaacata ttatccgggt tacggaaaag gcttttgatg
     4261 attttgcggc cagtggaacg gacgaaaaag caaaccgatt attgctcggg ttggattaac
     4321 cactaaattc gtacagcaaa aaaattataa tttaaaatat tttgtatgaa aaagatgatt
     4381 cttccattat tcgcgattgt tatgctggcc tcctgtggcg gagcagatac ttcaggtggg
     4441 aaaacgactg aacctgttgc cgaaaagccc aaagatccgg cttatgataa gggacttgct
     4501 ttagtggcac agaatgactg cctgacctgt cataaaatca atgaaccctt taccggaccc
     4561 agttattcgc aggtagcgga gaaatatgcg ggtgctgatg cggccaaaat aaaagaaatc
     4621 tccgagaaga ttatcaaagg gggtacgggt gtttggggat cagccctgat gacgccccat
     4681 gcctcccttt ctcaggaaga tgcagaagcg atggtaaaat atattttatt acttaaaaaa
     4741 taaccaagga tgaaaaccct gctcatcggc actatcgtag ccctgtctgc ggtctcgtgt
     4801 aacaacagtt ccgagactac gaaaaatgcg gaaggcgaca gcctggtgcc tccaacgact
     4861 gctatggcca atgtattaac ggaagaacag caaaaagaag gctggcaatt attatttgat
     4921 ggccaaacca ctaagggctg gcataagtat ggtggtagcc ctgttggcag tgcctggaaa
     4981 gtggccgatg gaatgctcta cctggatact accgataaaa aggactggca gatcagggat
     5041 gggggcgata tagtgagtga ttctgcctat gataatttcc atctcaaact cgaatggaaa
     5101 attgccaaag acggtaacag cgggatcatc ttttatattc atgaggatac cgctaaatac
     5161 gaatggccct ggaataccgg tcctgaaatg aaggtgctgg acaatagcgg acacccggat
     5221 tccaagatca ttaaacatcg ggccggggat ctgtatgacc tgatctcctg cagtaaggaa
     5281 accgtgaaac cggcgggcga atggaacctg gcggaaatca aatcacagaa tgctaaactg
     5341 gaactatcac tcaatggtga agtagtggtg agtaccacac tctgggatga tgcctggaaa
     5401 aaactcgtgg ccggcagtaa gttttccaaa atgcctggtt tcggtacgta tcagaaagga
     5461 aagatcggat tgcaggacca tggcaacatg gtatggtacc ggaatgtaat gatccggaaa
     5521 ttataagttg actgatatga atgataaaag gctggccatt ggccagcctt ttatcattat
     5581 tcgactttag cgatcttcag cacattcgtc ggcaggtgca ggtggatcgg cgtggagccg
     5641 gtactgacca ccacatcgcc gctacggaca aagtctcttt cttttaagat atcgatctgg
     5701 tcggaaaaga tatcatccag gctttcctct tcatcataat agaaggcgcg taccccccag
     5761 ctcaggctca gctgattgac gagtgagcgg atttttgtga agatatacag gtgagctttg
     5821 ggacggtaac tgctcagcat aaatccggta tacccactct gggtcatccc gatcagggcg
     5881 tcggcattta catcttcagc cagtttacag gcattataac aaatcgcatc actcagaaaa
     5941 gaaggagagt gcgattgggg tttcagcatt tcctcccggt tgtaatcgta ggcttttgtt
     6001 tccacttcca tgataatctt ggtcatggtc tccactacca gtttgggata cttgccggtg
     6061 gccgtttccc cactcagcat caccgcatcg gcgccttcca gtaccgcatt ggccacgtcg
     6121 gtgatctcac tccggttggg tttggtccgt tcgatcatgc tctccatcat ctgggtggct
     6181 acgatcacag gtttggcccg gtgcaggcat tttttgatta tatctttttg gatcagggga
     6241 atcgcttcta ccggcagttc cacaccgaga tccccccggg cgatcatgat gccatcactt
     6301 tccacgataa tatcacgcag gttcacaatc gcctgcggca tttcaatttt ggcaatcact
     6361 ttggcagagt ggcctgcttt tttaatcttt tctttcaaac cggtaatatc atccggactg
     6421 cgtacaaaag aaagggcaat ccattccacc cgttgtttca tgataaattc caggtctgcc
     6481 agatcttttt ctgtcagcga tggcagtgaa atgcgggtat ccggcagatt cattcctttt
     6541 ttgggcaaca gcaccccacc cagggtcact tccactttta cttcctttgc ggcggtgatt
     6601 tccttcacga tcacttccag tttaccatca tcaaacagga ttttctctcc cactttcaca
     6661 tcggtgtgca gatcggggta ggagacatag atcctttccc ggttcccgat cactttttca
     6721 ttggtgaacg tgagtatatc cccttcatta atttccaggg caccgccttc caggtctcct
     6781 acacggagtt tcggtccctg caggtcagcg aggatggcaa tattgtaggg ctctgattta
     6841 ttgatcttgt gaatcagttc gatgatccgg gccttgtctt catgggcacc gtgagagaaa
     6901 ttcaggcgga aaacattcac gccggctttg accagttcca gtaatttttc ataggtatca
     6961 caggccggtc caacagtggc gacaatcttg gttcgtttaa aagcgtgtat acggccggct
     7021 tctttgtcaa atgatttatg gaggtatttg tcaatgtttt tattcataat aagtggcggt
     7081 ctttagtaga ggtgaaaagg cgtacggttc aatcagctgg cgaggatctt cacaatcttc
     7141 atccattcat cactgatctt acctttattt tttaccgctg tttccagcgg gatataattc
     7201 attttattat tctggatgcc gacgaataca ttatgacgac cttccagcag gcattcaacg
     7261 gcatgataac ccatccggct ggcaatcagg cggtccatac aactgggagc cccgccacgc
     7321 tgaatatgac ccagaataca aacccggatc tcggctttcg gtagtttttc ctgcagtacg
     7381 cgctgcactt cattggcccc gccaaactca tctccttcgg ctaccacgat caggttcacc
     7441 atttttttac gacgttcctt ttcctgcagg gaagaaatga tgccattgat atcggtttta
     7501 cgttccggta ttagaatgtt ttccgccccg gtggcaatcc cgctatgaag ggcaatatag
     7561 cctgcatccc gtcccatcac ttcaattata aagatccggt cgtgtgcatc catggtatcc
     7621 cttaccttat caatggcttc tacggctgta tttactgccg tatcaaatcc gatggtaaag
     7681 tcggtgcccg aaatatcttt atcaatggta cccgccaggc ccacacaggg aatatcaaac
     7741 tctttactga aatttaccgc accccggaag gaaccatctc ccccgatgat cacgagcccg
     7801 ttgatccctc tttttttgag gttttcataa ccggttttac gaccggcggg ttcataaaat
     7861 tccttacaac gggcggtttt aaggatggtt ccaccccgtt ggataatatt ggctacggaa
     7921 cggccttcca tccggaagat atcatcatcg atcagtccct ggtaaccccg catgatcccg
     7981 aatacttcca acccgtgata aatacctgta cgaacgactg ccctgattgc ggcattcata
     8041 ccgggggcat cacctcccga agtaagaacg ccgattcttg ttactttctt ttccatcttg
     8101 aattttaaaa cttggcaaaa ataggctttt gttttgtaat gtgtaccagg cacacaatcg
     8161 ttagtattaa acagttttcc aaatatctga tttattcttt ttatttattg aataaaccaa
     8221 tattggagac aaaaaagtaa tattcaggaa ggaagatgat gaactgaaat tgttttgtta
     8281 ctgaataact gaacttttat gccatttatc ccggttttta taaggtcttc cactgtccgg
     8341 ttgcagctgc gttgaatcat ttatcttagt actttaataa aagtgcatat gaaaaaactg
     8401 cttttacttc tcggaatact gcctgtactg gcctcaggac agataaaata cccggttacg
     8461 gccaaaggaa cgaccgtaga taattatcac ggaaccacgg taccggatcc ttaccgctgg
     8521 ctggaagatg accggagtga ctccaccagg gactgggtga cccgtcagaa caaagtaacc
     8581 tttgattacc tcgggaagat acagtaccga aaacaatggc aggacagact ggaggatatt
     8641 aataattacg ccaaatactc atccccgttt cgccagggga cgaatttcta tttttataaa
     8701 aatgacggac tgcagaacca gagtgtgctt taccggcaga aaggactgaa tggtaaaccg
     8761 gaattgttac tggaccccaa ccagttttcg gcggaaggca caacctccct tgccgccttc
     8821 agtatttcta aaaacgggaa gtacgcagta gtgggtaaat cagcgggcgg cagtgactgg
     8881 cgtacctttt ttgtgatgga cctgaccaca aagaaatacc ttaaagattc actggcctgg
     8941 gtgaaagtgt caggagctgc ctggaaagga aacggcttct tttacagccg ttatccggca
     9001 ccggagaaag ggaaggaact ttctacaaaa aatgaaaatc accaggtatt ctatcatgtc
     9061 gtgggtacct cacaggataa ggaccagctg gtttatgaag acaaagctaa tccgcagcag
     9121 tttaattccg tttttacgga tgaatccgaa cggtatgtgt ttctcaccag caacgaccgt
     9181 ggaaaaggaa aggaaggaaa cagcttgtgg tattatgaca gcaaatcaac cgacaaaaaa
     9241 ttcaaacctt tgataaaaga tatcggtgat tttcagtata gttttataga agaggtaaat
     9301 gggaaattcc tcctgcagac caatgagggt gcccggaacc gccggatcat ccaggtggat
     9361 cccctgcatc cggaaaagga gaactggaca acctttatac cggaagtgga agaaaacatg
     9421 tcttcggtaa ccagtgccgg aagtaaatta ttcgtcacct atatgaaaga tgtaaccagc
     9481 cgggtgtatg tgtatgacct gggtggaaag ctggaacggg aagtgaagtt gcccggtctt
     9541 ggtacggtaa gtggtttcgg cggtgaacgg gaagatccct ttgtctttta tacgttcact
     9601 tccattacct atccgccgac tatttttaaa tatgatatcg ctacgggtga aagcagtctc
     9661 ttccgtaaac cagaagtgaa gtttgatccg gaggattatg tgaccgaaca ggttttttat
     9721 ccttccaaag acgggaccca gattccgatg tttatcgtgc ataagaaagg actggagctg
     9781 aatgggaaga acccggccat cctttatggc tatggtggtt ttaatatcag cagcacgccc
     9841 aatttttctt cggcacggat tgcctggctg gaacagggag gggtgtattg cattgccaat
     9901 ctccggggag gcagcgagta tggagaaaaa tggcatgaag ccggtatgcg ggagaaaaag
     9961 caaaacgtgt ttgatgattt tatcaccgct gcagaattcc tgattgcgaa agggtatact
    10021 tctccggatt acctggctgc acagggcgga tctaatggag gactgctggt gggaaccgtg
    10081 atcaatcagc gtcctgaact tttcaaagtg gcgattcccc aggtaggagt gatggatatg
    10141 ctccggtttc ataaattcac gattggctgg aactggatcg ctgaatatgg cagcagtgac
    10201 aagcccggag attttaaaaa tctttatagt tattcgccga tccataatat caaacccggt
    10261 atcaattacc cggccaccct ggtcaccacg gctgatcatg atgaccgagt ggtaccggcg
    10321 cattccttca aatatgccgc tacccttcag gaattttatc agggaaataa cccgttactg
    10381 atccggatcg atgtgaattc cgggcacggg gccagtaacc tgaaaaagtc attggaaacc
    10441 accgcggata tctattcctt tattttttac aatatgggac tgaagcctga atttaagctg
    10501 acggccgacg ggaaaaaagc attctgatca accggataac tgcaccgcta tgaaagtact
    10561 gatcaccggg gccaatggat ttctcgggca ctacctggta accgctttat tgcaacaggg
    10621 atttaatgta ttggcaacgg gcaaaggggc ttgtcgcctg gcagggcagg gaactaacgg
    10681 ttttgattat gcggaaatgg attttaccga tctttcatcc acccgtgata cggtcagccg
    10741 ctatgccccg gatattatcg tacatgccgg ggcggtcagt aaacccgatg actgcgaact
    10801 cgatcaggcc cttgcctggc ggatcaatac cggaggaacc catcatttgc tggaagcggc
    10861 ggcagcacac caggctttct ttatttttat ttccactgac tttgtatttg atggggagaa
    10921 aggaatgtat gcggaagaag accagacggt ccccgtgaat tattatggcc agaccaaact
    10981 ggaagcagag aaggccgtac accagtatcc gcacccatgg gccgtagttc gtacggtttt
    11041 ggtatacgga gagccgcttg ccggcagaag taatattctt tccattgtaa aagataaact
    11101 ggaaaaagga gaggggtata aagtggtgga tgaccaggta cgcaccccaa cttatgtagg
    11161 tgacctggcg gaggggatcg tctccattat tcaaaaaaag gccaccggaa cctggcacct
    11221 gagcggggcg gaggtactta ctccctatga aatggcatgt aaagcagcgg attttctggg
    11281 acttgacaaa ggcttactta cgagggtaac ggccgataca tttacgcaac ctgccaaaag
    11341 gccggcaaaa accgggttta atatttccaa agcaaaaatg gtattgggct ttaagccggt
    11401 gtcctttgcg gaaggtctgg ccaaaacttt tttgggttga aataatgctg atgaaaagaa
    11461 accggatatt attttaatgc aaactgaatt ttttttctcc ggcatttacg gggacggcta
    11521 atttattttc tactggcggt accgggcagt tgtacacatt actcacatac gcacaatagg
    11581 ggttataggc tttattgaaa tccagtaaaa aaggactgtt ctccaggtct ccggtgcgga
    11641 tatcaatata acggccgttt tcataggttt gttcaccatt gctgagatcg gtaaatggaa
    11701 taaagaggta atctttatat tcttccgact gcatcagttg ttgtgactga taaacagtaa
    11761 gccgcagggg tttgccactc atcgtgaaat gaagcagggc ataaacgcgg aaagtttttt
    11821 tgattttgcc ggaggtttcc atcgcaaacc agggcgcttc atagatccgt tccaccctgg
    11881 agggcatgag gaatgaagta tcgataggat agaactgcag tccggccctg tctttttcct
    11941 ttatcacccc gtgtgtattt atatactgat ttctgaaagc cgaaacagaa tccaggtatt
    12001 gtggaggttg tgccacgatt aaatggccag aaaaaagtaa tacgaataac aggctgtact
    12061 tcatacggca atttagttca tcatcagcac gttcacactt cgctgcagta tattaaatgc
    12121 aatttcggcc atcaggtttt ccttatccag ggtagggttg acttccgcaa tttcaaaaca
    12181 gcagattttc cggttttgca tgaatttact gatcaggtct tctgcttccc tttcacggag
    12241 accattccca accggtgtcc cggttccttt ggagatagac gaatcgaggc tgtccacgtc
    12301 aaaggataca aagatgtcgg tacaatcgga gagatagcgg atagcggacc ggaccacatt
    12361 ttccgctcct ttgttgcgca cttccttggt ggtaattact ttcatgccgt atttctcgat
    12421 caggtgccgt tcttcttttt caaagtcacg cagggagata aagaccacat tttccggaag
    12481 gaccttaggc gtgatttttc cgagggcttt cagctggttc cattgccggg tggtttcctc
    12541 atccaatgta tgcacggcac attcctcgtt gtcttcgttg atcgacacgg ccatgggcat
    12601 accgtgcata ttgccggaag gggtggtata cggtgtatgc agatccgcgt gagcgtcgat
    12661 ccagatcacc cccagtttac atttgggttt cgccatttta attcctgcta tcaccgcacc
    12721 tgccgtgcta tgatcaccac ttaatacgac cgggaagaaa ttattcttaa tggtttcagt
    12781 caccgattta ctcacccgtt catagatgga actcacccct ttgatccgtt tggcataagg
    12841 cgattcgatt ggttcataaa gcagtttatt ttccgtttgt attttttctg aagggaagtg
    12901 aataaagaaa ttgctcatga aatcaagggc ggcaatttta atggcttcaa tgccgagact
    12961 ggcaccacgg gtacctgcac ctatttcaga gggaacttca atcagtttaa tatttttcat
    13021 actggccagt gttggataat cattgccggc gtacggcaaa taaataaggt taaacaaata
    13081 gggtagcaga cccgattaat caggaatgta aaaattggcg ggcagggatt gctcggcaaa
    13141 ggtgaaaacg ggacgctgtg tggataatga gagtctcata gaagagtaca aagataacta
    13201 atctttgacc aaaatcaaat aacgttcgtt cgtattttgt attttttata atcttaatat
    13261 attgatattc aatattattg atgtgcggcc aggattggga tatgactgtg ataggccagt
    13321 tttttggtat gggaggtacg gaaaagggag gaggagttac taggatgttt ggggacggtg
    13381 accagtatat caatatcttg ttcctttaca aaactatcca gtgcttcata gaaatcttca
    13441 tttgtgataa agaagaattc ttttttaaaa tccgcaaaca tctggtcaac cttttctttt
    13501 tccagttgaa gcgcagcagg cagggttttg atcgcttctt tactcacatg tacaatatgc
    13561 agtttgggat cgagaagttt aagtaccgat ccgatcaact gggatggggt actgagttcc
    13621 acatgctgat agtccgtggc aaaagccact ttagtcacgc ccttgtagac cgcatccggc
    13681 ggtatgatca ttaccgggta caggttttta tcaatcagtt tgagggtatt gctgccaaac
    13741 attacctggc ggatcgctga tttgccggta atccccatca taaccagggt ggcccgcatg
    13801 gtatgggcaa gccgcgaaat attttcaatc aggtcgccac ccaattcatg ttcacattcc
    13861 acttgtcgta ccccggcttt gttaaattcc tttttcaggc tctccaaata ataaagggaa
    13921 atatcgaggt catcttcatt ttcataatta tgatagagta cggcagtggc gctttcattc
    13981 cacgccagca ttttagaggc ataacgcgcc gcattcaggg aggtttcaga aaagtcaaca
    14041 gggatgatga tacggtgcat ggttggtttt gtttcgggta gttaaaaata aggaataagg
    14101 aataagaaat aaggaattag gaaatggggg attgggcgct tcctttcaca ttccttattt
    14161 cttattcctt attcttaatt ttccgtatga atcagccgaa aatcagagaa gccctcgttt
    14221 ccgacatctc ccaaattcaa cgggtgcgga atgcggttaa ggaaaacaca ctatcggatc
    14281 cggcattggt cccggattcg gatgtagcgg attatatcac ccgacggggg aagggctggg
    14341 tttcgctgga gggggaagcc gtgaccggat ttgccattgt cagtgtttcg gatcagaatg
    14401 tatgggcttt gtttatcgaa ccgggttttg ataagctggg tattggcagg caattgcatg
    14461 ataggatgat ggactggtat tttgaacaaa ctcaggagac tatctggctg agtaccactc
    14521 ccgggaccag ggctgaaggt ttttaccgga aagcgggctg ggaggaagcg ggtgaatatg
    14581 gaaaggggga agtgaagttt attatgaccc cggaaaattg gtcaaaacaa aaaaaacttg
    14641 cgggttaaca ttttcaataa ctttaaaaca cccatttgga caacccattg aagtggttaa
    14701 tcgtcctgat aattacaggt aatgaaacac acaccatgcg aagaataggc tatttttttt
    14761 ctctttttcc gttgatcttt tttgcctttt gggtaaaaca gccgcctgca caaagtgatc
    14821 tcctgtccaa agccgatgga ccttatttga tttacaggaa ggatacttta ttcgtccata
    14881 caatttatga tgccggcggg gtcactaaac tccgcacgga taccttaaaa accggggcaa
    14941 aaaaagaaat tcagctcaaa gtacccaccg gtaatccgga tgagtttttc acggtgacgc
    15001 taaagtcacg gcttcagcag gaaaaggcag aataccggaa agcaccccgt caactggcca
    15061 tttccgatat ggaaggagac ctgaaagggt tcctgaaact attaaaaggg aacggggtga
    15121 tcgaccaaca gtttaactgg actttcggag atggacacct ggtgctgacc ggtgattttt
    15181 ttgaccgggg tgtacaggta actgaactcc tctggtttat ttacagtctc gaggaaaagg
    15241 ccaaagccgc agggggatat gtacattttg tgctgggcaa ccatgagatt atgaatatga
    15301 atggtgattt ccgttatgtt cagccgaggt atatggaaca tgccgccctt atgcaactcg
    15361 attatttatc actttttgat gagcagagtg aactggggcg ctggctccgg accaagaaca
    15421 tcgtggaaaa agtaggggag gtgctgtata tgcacggggg tatttcatca gaagtaaatc
    15481 tgttcaatgc cgcggctcct gtacttaatg agctggcccg accctattac ggagacacca
    15541 gttatctgta tcccaatgtg catgtagaga ttttgtacag tgaggtcggc ccattctggt
    15601 accgggggta ttatatcggt tctccaaagg ctaccgaagc ccagattgac agtaccctga
    15661 gtattttcgg cgccagatat atcgccaccg gacataccat tgcccgggat accatcagca
    15721 cctggttcaa taataaattg tttaataccg atgtgccgca cagcaagggt ttttctgaag
    15781 ggctgctgtt agaaaaggga aagttttacc gggtcaacga agccggggac agattcctga
    15841 ttgccggtta agtcacccac ccgtactgtc tgtaaaaagc ggacctgccc ctgcttcatt
    15901 ttaggtttta tgcgccttct cgcttaaatt tgccccactt taaaaagcac taatcaatgg
    15961 caagatcaat cgcgtttcgt gaagctatcc gggaagccct cagtgaggaa atgagaaggg
    16021 atgaaagggt gttcctgatg ggagaggaag tggcagaata caatggagcc tacaaagtga
    16081 gccagggaat gctggccgaa tttggcgcta aacgggtaat tgatacccct atttctgagc
    16141 ttggttttac cgcaatcggg gtgggtgccg cccagaatgg ccttcgcccg gtagtggaat
    16201 atatgacctg gaattttgcc gttttggctt tagatcaaat tttaaataca gcttctaaaa
    16261 tgctggcaat gagcggcggg caaataagtt gtccgatcgt gtttcgcgga cccaatggtt
    16321 cagccggaca gttaggagct cagcactcta ctgcttttga agccctttat gcaaatattc
    16381 ccgggattaa agtggtatca cccagtaatg gatatgatgc caaaggattg ctgaaacagg
    16441 cgatccggtt tgaagaagat cccgtcatgt ttatggaaag tgagcagatg tacggcgaca
    16501 aatctgaagt gcccgatgaa gagtattata tcgaactcgg aaaagcagat gtgaaaagag
    16561 ccggacgcga tgtaactatt gtttcgttta ataaaatgat gaaagtggcc ctcggggctg
    16621 ccgaggagct cgaaaaggag ggaataagcg ccgaagtaat cgatttaagg acaattcgcc
    16681 ccctggattg gaaaacaatt ctggaaagtg taaaaaagac aaatcgcctg gtaatcgtgg
    16741 aagagcaatg gccttttgca tcgatatctt ctgaaattac ctaccagatt cagaaggaag
    16801 gattcgatta cctggatgcg ccaattcgcc ggataaccgc tgctgacgcg cctttgcact
    16861 atgcccccaa cctcgtggct gctgcattgc cggatgtgcc ccgtaccgta aaattggtaa
    16921 aggaagtgat gtaccaggtc aagtaggaat tatacaaata ataatggccc ctgcccactg
    16981 gcaggggctt tttttttaca ggcatctaaa caataaaaaa aaattcggat atcgtgcaac
    17041 gatttgattt ataaagtgca tctttgtctc ggtttttcat aggatattgg attttaaaac
    17101 gaggctgggt t
//
LOCUS       DAOPMW010000030        16841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3328_length_16841_cov_10.999404, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000030 DAOPMW010000000
VERSION     DAOPMW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_16841_cov_10.999404"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2320
                     /locus_tag="PLU11_02915"
     CDS             <1..2320
                     /locus_tag="PLU11_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN58090.1"
                     /translation="VQGWQNAMLRNEANMNIGSAPAFTPQKIRDLYDHQDEEYWYFNE
                     IMVKGLKQNYNMSVSGGNESSTYMVSLGYMDQESNFTGNFGLQRYNFRTNLTTEYKNF
                     KLSSVMAYNRRNERTIAGGTGNIIINSTRIPPYYYYKFTQDGKYLVNDVIGDDNTMAK
                     LKEGGHEDKDEDNFVGSLNLDYRIFNGLTAKGLVGLDLTQHHRYRRDIKVPLYSSADL
                     ENPVVYINPNSLTEDYNNKRHTISTQFFLDFDRTFNSDHNVTGLIGVSNESYTFKASR
                     IAWEYTDDDLGLPTTDASKQSTGNHNSNAATDQTSISSLFGRAGYNYADKYYTDLSFR
                     YDGSSKFAKDYRWGFFPSFSAAWRISEENFMGNYKTNVGDLKLRTSYGVLGNQNVDNY
                     SYQTVYQMYTNSYVFNNLPVPGTGYTYGNPFLTWEKSANFNFGIDATFLKNNLFVSLD
                     YFRKKTWDILLTPEVSSVFGSSAAKENAGTMQNRGWEATINYKLKSGDFSHNFNLNIS
                     DTKNEVTDFGGKERIDQTDQLYKLIREGEALGSYFGYKTDGLFQSYDEIANSALPLGA
                     SVHPGDVKYVDTYKDGVIDEKDRTVLGNAFPRYTFGFIYDLTYNNFDFNVLIQGVGKR
                     DMYVRGELVEPFHSNYSYAIYKHQLDFWTPTNPEAKWPRLVAPGSPSSANNWGKAGTD
                     IYLLDGAYLRVKNIQLGYSIPRQFTSKFGVQKLRISVNAQNALTLSKNSFIDPESSEF
                     GNNMGGIGGVGANSARNYPTLVYYGFGLDLEF"
     gene            2334..3953
                     /locus_tag="PLU11_02920"
     CDS             2334..3953
                     /locus_tag="PLU11_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN58091.1"
                     /translation="MKKGFFKYYIGIFSLILVVLPSCNELDLAPTNKFTDLNYWTSPE
                     KASAVLSMAYSQMFGANYFFANERLSDNLYEGRGNTDEKIITSGQADAALGRFANEWR
                     NCYEGIKTCHTFLENIDRVGGLDETLKGRMIAEARFIRASLFFRLTTHYGDVPLFKAN
                     LSLQEANTIARTPKADVIDFVRKELNEIVTILPTRQHYSASDNGRITKGAVMMLLART
                     YLYENDWANVASVSEKIMNGEYGQYALFPSYPGLFLPENEYNSEVILDMGYTLNLRTW
                     GEFYDAIPISVGGRINAFAPTQELVDDYIMKNGKAIHETGSGYNEDNPYSNRDPRFAA
                     TIVYHEYKWIKGNGTSSTIYIKPGSSKEAGASNLDEYAGPGQNSTGTGYYLRKYFDPT
                     APASLQAGLNLILMRYADVLLMYAEAKNELGQMNEEIWNKTIKPIRQRAGFTDASALN
                     YPGTDLRNIIRRERRCELAVEGLRLFDIRRWKTIETVMNGNPHGAKFAANNTQLIQLD
                     MRRFNKDRDYLFAVPQSQRDINPNLTQNNGY"
     gene            3997..5121
                     /locus_tag="PLU11_02925"
     CDS             3997..5121
                     /locus_tag="PLU11_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HPN58092.1"
                     /translation="MKTIGPKIILLISLILAFFACSDDGEVRDIGVTPVKKLYEPDNG
                     KAVVLQSSASAILYFEWEPARAENSGTVLYEVVFDKADGNFSNPIYTTASGNNGGANH
                     AMISHKVMNRIAALAGIESSAKGTIKWTVYSSKGIDPVKAEEERMLTVTRLAGFADIP
                     NQLYITGAGTEAGGELSNALIMKKITDGEFEIYTKLTAGQPFKFVSSNTGSPVEYSLS
                     GEKVVEKGTSTVTKTGIYKYYIDFNIGAFTTKEVTKVNLFLNWSQRKIELPYKGFGIW
                     ELTNHTITGLSGNDNNDDRYKFRMESSKGETEWRAINNDSKPTGNDAYYYMVEKTNVE
                     QWTNNQIWKNPSTTGWNDKTYDIMFSLNPKNEYTHNLVIK"
     gene            5136..6275
                     /locus_tag="PLU11_02930"
     CDS             5136..6275
                     /locus_tag="PLU11_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HPN58093.1"
                     /translation="MKNILIKTGCLAALVLILMACNDTMGDPDYRLTEVKALIEPANG
                     KTIKLEPLPSASVYFEWEYIEVEKGGTAVYQIAFDKPDGDFSDPVYMVNSGNNGFNNH
                     VTLSHKQLNKIAGMAGIFPSETGSVKWTVFSSKGTKAMKAASENTITITRLAGFEEVP
                     IDVYVTGEGSEGGTDLSKAQKMKAVAGGEFEVYTKLKANSPYYFTDSRSGTPRKFYTA
                     DGLVKENGSSKVPTEGIYRITLDFNTGASTYTLVLGIGFYFSPAGSLLFELPYKGNGI
                     FQAKETVTFKQEGWGRDERYKFRMFVKENSGAGEVKELEWGTLNQTDSRPTPTTAESY
                     YYLKLLTNLTQWDNKWKLMGDFDGVPAVYTIYLQADKPYTHSVTK"
     gene            6367..7497
                     /locus_tag="PLU11_02935"
     CDS             6367..7497
                     /locus_tag="PLU11_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HPN58094.1"
                     /translation="MRKIFLILFLLQPWLFISCKEDISNNDNDQDKEEKIDWNVAANS
                     SSKALVSSFWNVKEKCFNTFSTGVLNFQYWPQAHALDVIIDAYLRTGDVTYKNYISQW
                     FEGVKVKNGNTFKNDYYDDMEWNALAILRAFKVTGDVKYKEAALEIWEYIKVGWNENA
                     GGGITWKKGMEYSKNACSNGPACILAARLYQEFGDEKNKDWALKIYNWEKSTLFNSNN
                     GIVYDNINSETGKIQNWIFTYNQGTFIGSAVELHKIFNEKSYLNDAVLAANYTISSLV
                     DNSILKSEGTGDGGLFKGIFVRYFTDLIQQDRLDATDKKRYLLFMKHNAETLWTQGTT
                     LPEVFFGPNWRVKPGSSTGLTEQTSGCMMIEAAAHLQNSGML"
     gene            7707..9860
                     /locus_tag="PLU11_02940"
     CDS             7707..9860
                     /locus_tag="PLU11_02940"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPN58095.1"
                     /translation="MNKHLLLFTCMFIICGCRTDKKLTGYVNPLLGTATLWEPEDLGY
                     VRKIRERTWGAEVFPGASLPNAMVQLSPVTQFRSGAGYQYEDTVIYGFAHTNKGHWNL
                     LHIPLLPVTGNITPQDYRSGFSHENETAHPGYYQVFLKRYRINAELTSTLRCGYHRYT
                     FPPGEEKKLVADMTRSNNRVTNWKIEKTGDHLFTGFQQAEGKLYFYALSNYPVNNIEQ
                     LKEDDREISLVTFGDSKGKKPLELKIGFSFVSVENAQMNLEQEMIDKPFSRVRKEADK
                     TWESLLSKIEVTGGTEREKGIFYSCLYRSFLWPALRSDVNKDYIDERNHVVNNGFHYY
                     TNPSFWDDYRNKLILLGMISPEVTLDVIQSLTDKGEKRGGYMPTFFHGDHASVFIAGS
                     YLRGLKDFDLERAYKLLLKNATVPGRGGRPYLDEYMERGWIAEKDTTNVPTWDEYKAA
                     VTKTVEYAYDDYATALIAKELGDEENYRMLMGRTGNYKNLFDPGTGFWRGKIDNGDWI
                     EDFDPFYPYYAYMFREANAWQSLFFAPHDVDGMLALYPSHQAVELKLDSLFTVPWKGY
                     EAHNMTGFIGNYCHGNQPDHHIPYTYTFIGRQEKTQAVLDSIMGRFYDMGADKLAYAG
                     MDDAGEMSAWYVFNAIGLYTYSPADPEYMVTVPLFDKIRFTLGDNTTFTIRKSGNGKK
                     IQGITYGGNKLEGWFISHRQLQEGKELTVQTEDIR"
     gene            9999..13517
                     /locus_tag="PLU11_02945"
     CDS             9999..13517
                     /locus_tag="PLU11_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN58096.1"
                     /translation="MSGSFGKTWWGEKWLQSLNNIDFSNRLPRGASYARKGAVEKITI
                     TGNHISAKVKGSRPRPYKVDIIMPPFFDPELENFLRDLTRQPVIISRLLNRELDPSLL
                     DLAMKHGLKVFPRQWSDLKMQCSCPDWAVPCKHLAAVIYKVSSEIDNNPFQVFTIHNV
                     DLLRRLDQLGIVIHKENMEIPGIKELCLEKKKSATKFDPENAYNKLSFTGLQSLHEPL
                     TALLSPSPAFYTTSETDFREKYRALLTRVVKSTQKAAKGKIALEEFFSGASNIEQQIN
                     PYSELTIVLNDQFRAQVWIDDLKYTIPELLLQLSRIHSSRIYDYQPSTAALHTALHLS
                     LQLVAHGAIVPQIVRLTGKAYIVRWLPAMISSDVKKLVDELENILLPDLCYWQEGEKL
                     TVINREPALNLLSLFITEIVRIVSYRVEDNLFESLFFKSAAYPFNLPGEESLPGGIQV
                     WLQKYYLTQGRFKPMLLVEETRDNRFIVQVNIQDRNHPNEASTPLQTILKDEQYGNER
                     FEILQSLTQLSAFISGLDTYINAGGKEDLIMTNEVFTRFLMEMIPAIQLLNISVLLPI
                     SLQEILRPKASVKLKSKPSARGFLRLDKLLDFDWQIAIGDTLLPPEEFEKLLKRSEGL
                     IRFKKRYIYVEKEDLEKIHRHLSQPGEISAFQMLRAALSGEYQGAPVSMTDEVKEMVS
                     ELINVGEVKLPEGLQALMRPYQHRGFSWIYRNAQIGFGSVLADDMGLGKTLQVIATIL
                     KFKEERVLEKEKVLVVAPTGLLTNWQAEIQKFAPSLLTEIYHGTTRDLKRLSEVDILI
                     TSYGMARRDADQFKKMKWHTLVIDEAQYIKNQETQQTKAIKSIKAGNCIALSGTPVEN
                     RLSELWSIMDFSNRGFLGSLKEFQDTWAQPIEQLNDRETANKLKKVTAPFMMRRLKTD
                     KNIISDLPDKIEIDSYASLTSVQAGLYEKTLDEAMDAIEGVSGTDQKALFVRQGLVLQ
                     MILALKQICNHPAQFLKNGETDASLSGKVELLFDKLDSITESNEKVLLFTQFTQMGDL
                     LRHFITERYHNEPLYIHGGCSIDQRKEMVERFQNNHADRIFLLSLKAAGTGLNLTAAS
                     HVIHYDLWWNPAVEAQATDRAYRIGQKNNVMVHRFITRNTFEEKINEMIRKKKALAEM
                     TVATGESWIGNLSNKELREIFTPGEK"
     gene            13566..14321
                     /locus_tag="PLU11_02950"
     CDS             13566..14321
                     /locus_tag="PLU11_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HPN58097.1"
                     /translation="MTPKKLPYAPLQEVIFEGKWELSIDPHTKTFVDPEYPFALGKFQ
                     QSINSKFPVYKVKIPGGIPFQMLSHQPVHQFWSKDGIWPVIQYGPGVVTLNDTEKNYT
                     WEDTFFPLAKDTLLNIKNAYSTELAFISYTLRYIDVVNIKNYKFTNWMDFVKENINVE
                     FNNNFIPPGPLSQFNFEQTFEINEVGTLEISFSNGQDHKNEEIFIWQNAVSGKNALTC
                     EELVSWLSKAHGFCSNIFKEICKKDFYDSFISR"
     gene            14299..14787
                     /locus_tag="PLU11_02955"
     CDS             14299..14787
                     /locus_tag="PLU11_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58098.1"
                     /translation="MIVLLADKPEQASMSTILKPDNEEIFPKWFVKRRNYVAPNSTVF
                     PDITVFSGDIIDLLQKLNSFTSLKYNWDSYGAIPPSLMSIRNARRFLFKNANVSLPFY
                     FVSPGINGEVMIELNKDQKAAEIYFNPDGSEELLLYENNNCILEKEDVSSYTDLIEFF
                     NE"
     gene            14780..15145
                     /locus_tag="PLU11_02960"
     CDS             14780..15145
                     /locus_tag="PLU11_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58099.1"
                     /translation="MSESLPQITIEDNDRLLRRVLFLHPDFIKPDGTPSSSSFSLKTG
                     EKGLSVDIERLTTYDRSILDKSRFRLYFLKASFTTQLGLTNQHDPLPDNYAHALITGN
                     FSRSICRLLASFAKRINFP"
     gene            complement(15286..16701)
                     /locus_tag="PLU11_02965"
     CDS             complement(15286..16701)
                     /locus_tag="PLU11_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58100.1"
                     /translation="MTKIIGRLKEQNELQRYYQSDTSEFIAVYGRRRVGKTFLIREFF
                     NNTFDFYLTGLANANLASQLLNFQFSMRRAGLTHAPVVKNWLCAFQQLIELLEKSRNK
                     RKVVFLDELPWMDTPKSDFMSSLEHFWNGWASGRSDIVLIVCGSATSWMMNKLLNNKR
                     GLHNRITQRIPLRPFTLLECKQFLQSVEISWEEYQITECYMIFGGIPYYWSLLRKGLS
                     LAQNVDYLFFHESGALRNEFGNLYAALFRNSDKYVQLMETISRKSKGLTRGELIKSGK
                     VKTGGGLSKMLEDLENCGFIRSYLSFGKKSRDKIFQVTDFFTLFYYHFLNLNFASSEN
                     HWSTMIDTPQHRIWSGYAFERVCVVHIQQIRQSLGISGVHCRYASWRSKGTGNGAQID
                     LLIDRNDSVINLCEMKYSIHPFAIDKKYAANLRNKIGTFKEETQTSKSVFLTFITTFG
                     VKQNEYAGMVQSEVKMADLFV"
BASE COUNT         5061 a         3782 c         3701 g         4297 t
ORIGIN      
        1 ggtgcaggga tggcagaacg ccatgttgcg aaacgaagcc aacatgaaca tcggcagcgc
       61 tcctgcattc acccctcaaa aaatccgtga tctatatgat caccaggatg aggagtactg
      121 gtatttcaat gaaataatgg tgaaagggct gaagcagaac tacaacatga gtgtttcggg
      181 gggaaacgag agttcaacct atatggtttc cctgggatac atggatcagg aaagcaattt
      241 taccggcaac ttcggtctgc aacgttacaa tttccgtacg aacctgacca cggagtacaa
      301 aaatttcaag ttatccagtg ttatggctta taaccgccga aacgagcgga cgatagccgg
      361 aggtaccggt aatatcatca ttaactcaac ccgtatccca ccctattact attacaagtt
      421 cacgcaggac ggcaaatacc ttgtaaatga tgtaattggc gatgacaata ccatggccaa
      481 acttaaagag ggaggccatg aggataaaga cgaggataat ttcgtcggaa gtttaaacct
      541 cgactataga atttttaatg ggctgaccgc aaagggactg gtcggactgg acctgaccca
      601 gcatcaccgc tatcgcaggg atattaaagt tcccctttat tcttcagccg atctggagaa
      661 tccggtggtc tacatcaatc cgaattccct gacagaagac tacaacaaca aaagacatac
      721 tataagcacc cagttcttcc tcgattttga tcgcaccttt aattccgacc acaatgtcac
      781 cggattgatc ggtgtttcga atgaatctta cacgtttaaa gccagtcgca ttgcctggga
      841 atacaccgat gatgacttgg ggcttcctac tactgacgct tcaaaacaga gtaccggtaa
      901 ccataactct aatgcagcta ccgaccagac cagcatttcc tctctttttg gtagagcagg
      961 gtacaattat gccgacaaat actatactga cctttcgttc cgctatgatg gctcctctaa
     1021 attcgcgaag gactaccgct ggggattttt cccatccttt tctgctgcat ggcggatttc
     1081 ggaagagaat tttatgggaa attacaagac gaatgtggga gatttgaaac tgcgtacatc
     1141 ctatggggtt ttagggaacc aaaatgtaga caactattcc tatcagactg tttaccagat
     1201 gtacaccaat tcctatgttt ttaacaacct gccggtaccg ggtaccgggt acacttatgg
     1261 aaatccattt ctgacctggg aaaaatctgc aaatttcaac ttcggaattg atgctacttt
     1321 cctgaagaac aaccttttcg tgtcgttgga ttatttcaga aaaaaaacct gggatattct
     1381 tttaacaccg gaagtctcct ctgtcttcgg gagttcagcc gctaaggaaa atgccggaac
     1441 catgcaaaac cgcggttggg aggcaacaat caactataaa ctcaaatcag gtgatttcag
     1501 tcataatttt aatctgaata tctcggatac caaaaatgaa gttaccgatt ttggcgggaa
     1561 agagcgtatt gaccagaccg accagcttta caagctcatc cgggaaggtg aagccctggg
     1621 atcctatttc ggctataaaa ccgacggatt attccagagt tatgatgaaa ttgccaacag
     1681 cgcccttcct cttggcgctt cagttcatcc gggagatgtt aaatatgtgg atacttataa
     1741 agatggggtg atcgatgaaa aggaccgcac ggtactcgga aacgcattcc ccagatacac
     1801 cttcggattc atctacgatt taacctataa taacttcgac ttcaacgttc tcattcaagg
     1861 tgtcggaaaa cgtgacatgt atgtacgggg tgaactggtg gaacccttcc attccaatta
     1921 ctcctatgct atttacaagc accagcttga tttctggaca cccacaaatc ctgaagcaaa
     1981 atggccccgg ctggtagccc ccggatcgcc ttccagcgcg aacaactggg gaaaggcagg
     2041 caccgatatt tatcttttag acggagctta cctgcgtgta aaaaacatcc aactgggata
     2101 ttccataccg agacaattca cttctaaatt cggcgttcag aaattgcgta tcagcgtgaa
     2161 tgcacagaac gcattgacgc tgtcgaaaaa ctcttttatc gatcctgagt cttcagaatt
     2221 cggtaataac atgggtggca ttggtggtgt tggtgcaaac agcgcccgta actatcctac
     2281 tttggtatat tatggattcg gattggattt agaattttaa aaaggttgat attatgaaaa
     2341 aaggattctt taaatactat atcgggattt tcagcctgat cctcgtggtg cttccctcat
     2401 gtaacgaatt ggatttagcg cctacgaaca aattcactga cctaaattac tggacctcac
     2461 ccgaaaaagc ttcggccgtc ctgagtatgg cttatagtca gatgttcgga gccaattatt
     2521 tctttgcaaa tgaaagactc tctgataacc tatatgaagg caggggaaat acggatgaga
     2581 aaatcattac ctccggacag gctgatgccg ccctgggtcg ctttgccaac gaatggagaa
     2641 actgttatga aggcattaaa acatgtcata ctttcctgga aaacatagac agggttggag
     2701 gcctggatga aacattaaag ggaagaatga tagctgaagc ccggtttatc agagcctccc
     2761 tgtttttcag actaaccact cattatggag atgttcccct gtttaaagca aacctgtcat
     2821 tacaggaagc caataccatt gcccgtactc caaaagccga tgtgatagac ttcgtcagaa
     2881 aggaattgaa tgagattgtg acaatattac ctaccagaca acactattca gcctccgata
     2941 atggcaggat taccaaagga gccgtcatga tgctgctggc gcgtacctac ttatatgaaa
     3001 acgactgggc taatgtagct tcagtaagtg aaaaaatcat gaatggcgaa tacggacagt
     3061 atgccttgtt tccgagttat ccagggcttt tcttacccga aaatgagtac aacagtgaag
     3121 tcattcttga catgggatac actctcaacc tccgaacctg gggcgagttt tatgatgcaa
     3181 tcccaatttc ggtaggaggg cgcatcaatg catttgcccc aacccaggaa ctggtcgacg
     3241 attacattat gaaaaacggg aaagccatcc acgaaacggg ctccggttac aatgaagata
     3301 atccttacag caaccgggac ccacgctttg ccgcaaccat tgtttatcac gaatataaat
     3361 ggataaaagg taacggcacc tcctcgacga tttacatcaa acccggttct tcaaaagagg
     3421 caggggcttc caatctggat gaatacgccg ggccgggaca gaactccacg ggaacgggtt
     3481 attaccttag gaagtatttc gatccgacgg ctccggccag tcttcaggca ggattgaatc
     3541 tcatccttat gaggtatgcc gatgttttgc tcatgtatgc agaagctaaa aatgaattgg
     3601 gacagatgaa tgaagagatc tggaacaaaa ccatcaagcc gatcaggcag cgtgccggat
     3661 ttaccgatgc ttctgcattg aactatcccg gtactgacct ccggaatatc atcaggcggg
     3721 agcgccgatg tgaattggct gtggaaggct tgagactttt cgacatccgc cgttggaaaa
     3781 ctattgaaac cgtcatgaac ggcaatcctc atggcgccaa gttcgcagcc aataataccc
     3841 agttgatcca actggatatg agaaggttta acaaggacag ggattatctg ttcgcagttc
     3901 cccagtccca aagggatatc aatccgaatc tcacgcaaaa caatgggtat taattattat
     3961 gtggtttcaa cgtatcaatt tataaattgt ataattatga aaacaatagg tcctaaaata
     4021 atcctgctga tatctctgat tcttgctttt tttgcctgca gtgatgatgg ggaagtcaga
     4081 gatattggcg tgactccggt aaaaaaacta tatgaaccgg ataatgggaa agcggttgta
     4141 ttacaatctt ccgcttcggc aattttgtat tttgaatggg aacctgccag agctgaaaac
     4201 agtggaaccg ttttatatga agtagtgttt gacaaagccg atggcaactt ctccaatccg
     4261 atttatacta cggcttccgg gaataacggc ggagctaatc atgccatgat ttcccacaaa
     4321 gtaatgaata gaattgctgc attggcaggg atcgaatctt cggcaaaagg taccattaaa
     4381 tggactgttt actcttcaaa aggtatagat cccgtaaaag cggaggaaga gcgcatgctg
     4441 acagtaacgc gtttggccgg cttcgctgat attcccaacc aactctatat tactggagca
     4501 ggaacagaag ctggaggaga actcagcaat gcactgatca tgaagaagat taccgatggg
     4561 gaatttgaaa tctataccaa attaactgcc ggtcagccct tcaagtttgt cagttctaac
     4621 acaggttcac ccgttgagta ctccctgagt ggggaaaaag ttgttgaaaa aggaacttct
     4681 accgtgacaa aaacaggtat ttacaaatac tatatcgatt ttaacattgg tgcgtttact
     4741 accaaggagg tcaccaaagt gaacctgttc cttaattggt cgcagcgaaa aattgaattg
     4801 ccctataaag gatttggaat atgggaattg accaaccaca ccattaccgg cctttctggg
     4861 aatgacaaca atgacgaccg gtataaattc agaatggaaa gttccaaggg agaaaccgag
     4921 tggagggcaa tcaataatga cagcaaacct accggaaatg atgcctatta ctacatggtg
     4981 gaaaaaacca atgtggaaca gtggaccaac aaccagatct ggaaaaatcc atccacaact
     5041 gggtggaatg acaaaactta tgatatcatg ttctcattga atcccaaaaa tgagtacacc
     5101 cacaatttag ttataaaata atgaggataa atattatgaa gaatatacta ataaaaacag
     5161 gctgtttagc tgcgttggtt ttaatactga tggcatgcaa cgacacgatg ggcgaccccg
     5221 actaccggtt aacagaggtt aaagcgctaa ttgaacctgc caatggcaag accatcaagc
     5281 tggagccttt gccttcagcc tctgtatatt tcgagtggga atacattgag gtggaaaagg
     5341 ggggtactgc tgtttaccag atagcttttg acaaacctga cggggatttt tcagatcccg
     5401 tgtacatggt taattccggg aacaacggtt ttaacaacca tgttaccctg tcacataaac
     5461 aactgaacaa gattgccgga atggccggaa ttttcccatc agagacaggt tcggttaaat
     5521 ggacggtatt ctcttcaaag ggcacaaaag ccatgaaggc agcttcggaa aacaccatta
     5581 cgattacgcg cctggcaggc tttgaagaag tacccattga tgtgtatgtt acaggagaag
     5641 gttccgaagg gggtactgac ctttcaaaag ctcaaaagat gaaggccgtc gcaggaggcg
     5701 aatttgaagt ctatactaaa ttaaaagcca acagccccta ttattttacc gacagtcgca
     5761 gcggcactcc ccggaaattt tacacggccg atggactggt caaggaaaac ggatcctcga
     5821 aggtaccaac tgaaggtatt tacaggataa ccctcgattt caataccggg gccagcacgt
     5881 ataccctggt gcttggaatc ggattctatt tcagtcccgc cgggagtcta ctgtttgaat
     5941 taccctacaa gggaaatgga atattccagg ccaaagaaac ggttaccttt aaacaagaag
     6001 gctggggaag agatgaacgc tataaattca gaatgttcgt aaaagagaat agcggagcag
     6061 gggaagtgaa ggaactggaa tggggtactt taaaccagac cgattcccgc ccaacaccaa
     6121 ctaccgctga atcttactac tacctcaaat tactgacaaa cctgacgcaa tgggataaca
     6181 agtggaagtt aatgggtgat ttcgacggcg ttcctgcggt ttacaccatt tatcttcagg
     6241 ctgataaacc ttatacacac tccgtaacca aataataccg gcctgagtat aataaaaaga
     6301 gataagagtt catgttgccg aactcttatc tctttattct ccaatcaatt ttcgaagacc
     6361 tataacatga ggaaaatatt cttaatactc tttttgttac agccatggct gtttatttca
     6421 tgcaaagaag atattagcaa taacgacaat gatcaggata aggaagaaaa aatagactgg
     6481 aatgttgccg ccaacagcag cagcaaagcg ctggtaagct cattctggaa tgtcaaagag
     6541 aagtgtttta atactttcag cacgggtgtg ctgaattttc agtactggcc acaggcacat
     6601 gccctggatg tcatcattga cgcctatttg cgaaccggtg acgtgactta taaaaattac
     6661 ataagccaat ggtttgaagg ggtgaaggtc aaaaacggga atacttttaa gaatgactac
     6721 tacgacgata tggagtggaa tgcattggct atcctccgcg ctttcaaagt caccggcgat
     6781 gtgaaatata aagaagccgc actcgaaatc tgggagtaca taaaagtcgg gtggaatgag
     6841 aatgccggtg gtggtatcac ctggaaaaaa ggaatggaat acagtaaaaa cgcctgttcc
     6901 aatggtccgg catgcatcct ggctgcccgc ctttaccagg agttcggcga cgaaaaaaac
     6961 aaagattggg ctcttaaaat ttacaactgg gaaaaatcaa cccttttcaa cagcaacaac
     7021 gggatcgttt atgacaatat caacagcgaa accggcaaaa tccagaattg gatttttacc
     7081 tataaccagg ggactttcat tggttcggca gtggaattac ataaaatatt caatgaaaaa
     7141 tcttatctga acgatgcagt tttagcggca aactatacca tcagcagttt ggtggacaat
     7201 tccattctta aaagtgaggg aactggtgat ggcggtcttt ttaagggtat ttttgtcaga
     7261 tattttaccg acctgattca acaggacaga ctggatgcca ccgataaaaa acgatacctt
     7321 cttttcatga aacacaacgc ggaaaccttg tggacgcaag ggacaacgct tcctgaggtt
     7381 ttctttggcc caaactggag ggtgaaaccg ggttcctcta ccgggttgac cgaacagaca
     7441 agcggatgca tgatgatcga agcagcggcc catttgcaaa attccggaat gctgtaatca
     7501 ttcgccttat tcacatattg tcctttacaa aactgcgttt caattacata tcgaaagcat
     7561 cacggatcgg ctcattaccc aaatggaatg agtttcgcga tactccgctg aacttcgttt
     7621 cacatgaacg aacaaataca gcctttttgg taaattataa caaatccgta aaattattgg
     7681 aatcatgacg aattaacagt tcacttatga acaaacacct gctcctcttt acctgtatgt
     7741 ttataatttg cggttgcagg accgacaaaa aactcaccgg ttatgttaat ccattgctgg
     7801 gaacggcaac gctatgggaa cctgaagatc tgggatatgt ccgcaaaatc agggaacgta
     7861 cctggggcgc tgaagtgttt cccggcgcct ccctgcccaa tgccatggtt cagcttagtc
     7921 cggtaaccca gttcagaagc ggtgccggat accagtatga agatacggtc atctacggat
     7981 ttgcccacac caacaaaggg cactggaatc tgctgcatat tcccctgtta ccggttacgg
     8041 gaaacatcac ccctcaagat tacaggtcgg gattcagcca cgaaaatgag accgcacatc
     8101 ccgggtatta ccaggtgttt ctgaaacgct atcgcatcaa tgccgaattg acttcgacct
     8161 tgcgctgcgg ataccaccgg tataccttcc ccccgggaga agaaaaaaaa ctggttgccg
     8221 acatgacccg ctccaataac cgcgtgacca actggaaaat cgagaaaacc ggcgatcatc
     8281 tattcacggg gttccaacag gcggagggga aattgtattt ctatgcactc tccaactacc
     8341 cggtgaataa tattgaacaa ttaaaggaag atgaccggga aatatcgttg gtgaccttcg
     8401 gagatagcaa agggaaaaaa ccgctggaac ttaaaatagg tttttccttt gtaagtgtcg
     8461 aaaatgccca aatgaacctg gaacaggaaa tgatcgacaa gcctttttcc agggttcgca
     8521 aggaagcgga taagacctgg gaatcacttt tgtcaaaaat tgaggtgaca gggggaaccg
     8581 agcgggaaaa aggaatcttc tattcctgcc tttacagatc cttcctctgg ccggcactcc
     8641 gaagcgatgt caacaaggat tatatcgacg agagaaacca cgtcgtcaat aacggattcc
     8701 attattacac caatccctcc ttctgggacg actaccgaaa taagctcata ttgctgggga
     8761 tgatctctcc ggaagtaacc cttgacgtca tccaatccct taccgacaaa ggagaaaaaa
     8821 gaggaggata tatgcctact ttctttcacg gggatcatgc ttctgtgttt atcgctggat
     8881 cctatctgcg gggtttgaaa gattttgacc tggaacgggc ctataagctg ctgttgaaga
     8941 atgctacagt tcccgggcgt ggcggaaggc cttatctgga cgagtacatg gaaaggggct
     9001 ggattgccga aaaagatacg acaaatgtcc ctacctggga tgaatacaag gcagcggtca
     9061 ccaaaacggt ggaatacgcc tatgacgact acgccacagc cctgatagcc aaagaactgg
     9121 gtgatgagga aaattacagg atgctcatgg gacgcacagg caattacaag aatctgtttg
     9181 acccgggaac cggcttctgg aggggaaaaa ttgacaacgg cgactggatt gaagattttg
     9241 atccatttta cccttattac gcttacatgt tcagggaagc caatgcctgg caatccctgt
     9301 tttttgcccc gcacgatgtg gatggaatgt tagcccttta ccccagccat caggcggttg
     9361 aactgaagct ggattcgttg ttcacggtac cctggaaagg ctacgaggcc cacaacatga
     9421 ccgggtttat cgggaattac tgtcatggca accaacccga ccatcatatt ccttatacct
     9481 ataccttcat cggcaggcaa gagaaaaccc aggccgtact ggacagcatc atgggcaggt
     9541 tttacgatat gggggcagat aaactggctt atgccgggat ggacgatgcc ggagaaatgt
     9601 cagcctggta tgttttcaac gccatcggat tgtatactta ctccccggca gatcctgaat
     9661 atatggttac agttccgctg tttgacaaaa tccggtttac attgggcgac aataccacat
     9721 ttaccatcag aaagagcggc aacgggaaga aaattcaagg aattacctac ggtggtaata
     9781 aattggaggg atggtttatt tctcaccgtc agcttcagga aggcaaagaa ttaaccgtac
     9841 aaactgagga tattcggtaa aagccgccag ctcatcacgg aactgttcgt gtccgtgttc
     9901 gccgccattg gataaaacga acatctggta tcgaaaagat atgccgatta ttctattttt
     9961 gtgcctgtca acaaaattcc aactccaact tagaaattat gagcggatcc ttcggaaaaa
    10021 cctggtgggg agagaagtgg ctgcaatcgc tgaacaacat tgatttcagc aaccggctgc
    10081 cacgcggagc ctcctatgcc agaaaagggg cggtggagaa aatcaccatt accggcaacc
    10141 acatttcggc caaagtgaag gggtcgcggc cccggccata caaagtcgat atcatcatgc
    10201 ctcccttctt tgatcccgaa ctggagaatt ttctccggga cctcacacgg caaccggtga
    10261 tcatttccag gttgctgaat cgcgaactgg atccttccct gcttgacctg gcaatgaaac
    10321 atgggttaaa ggtttttccc cgccagtgga gcgacctgaa gatgcaatgt tcctgtcccg
    10381 actgggctgt tccctgcaaa cacctggctg ctgtcatcta caaagtctct tcggagattg
    10441 acaacaatcc ctttcaggtt ttcaccatcc ataatgtaga tcttctccgg agacttgacc
    10501 agcttgggat tgtgatccat aaagaaaaca tggaaattcc aggaattaag gagctttgtt
    10561 tagagaagaa aaaatccgca acaaagtttg atcctgaaaa tgcatataat aaactctcct
    10621 tcaccgggct tcaatccctc cacgaacccc tgacagccct tctttcccct tctccggcat
    10681 tttataccac gtcagaaaca gatttccggg aaaaataccg tgcactcctg acccgggtgg
    10741 tcaaaagcac ccaaaaggca gcaaagggaa aaatcgccct tgaagaattc ttttccggag
    10801 cctctaatat agagcagcag atcaacccct attcggaact cacgattgtc ctgaatgacc
    10861 agttcagggc acaggtatgg atcgatgacc ttaaatatac catcccggaa cttttattac
    10921 agctttcgcg gattcatagt tcccgcatct acgactacca gccctcgaca gcggcccttc
    10981 acactgcgct acacctctcg ctgcaactgg tcgcccatgg tgccattgtt ccccagatcg
    11041 tcaggttaac cgggaaagct tatatcgtcc gctggttacc ggcgatgatc agcagcgatg
    11101 tgaaaaagct ggtggatgag ctggaaaaca tattgctgcc cgatctctgt tactggcagg
    11161 aaggggagaa actcacagtc atcaacaggg aacccgccct gaacctcctt tcgctcttta
    11221 ttaccgaaat cgtacggatc gtcagttaca gggtggagga taacctgttt gaatcccttt
    11281 tctttaagtc agcggcctat ccttttaacc tgccagggga agaatccctg ccggggggta
    11341 tccaggtctg gcttcaaaaa tattacctta cacagggcag gtttaaaccc atgctcctgg
    11401 tggaagaaac cagggataac aggtttattg tccaggtgaa cattcaggac agaaaccatc
    11461 caaacgaagc gtccaccccc ttgcagacca tactcaagga tgaacaatat ggaaatgaac
    11521 gttttgaaat tttgcaatcg ctgacccagt taagcgcctt catttcggga cttgacacct
    11581 acatcaatgc cggtggaaaa gaggatctga tcatgaccaa tgaggttttt acccgtttcc
    11641 tgatggaaat gattccggcc atccaactcc tcaacatttc agtattgctg cccatatctt
    11701 tgcaggaaat attacggccc aaagccagtg ttaaattgaa gagcaagcct tcagccaggg
    11761 ggtttctccg gcttgataaa ttgcttgatt ttgactggca gatcgccatc ggtgatacac
    11821 tgctgccccc ggaggagttt gagaagctcc tgaagcgatc cgaaggactg atcagattta
    11881 aaaaaagata catctatgtt gaaaaggaag atctggaaaa gatccaccgc catttatccc
    11941 aaccggggga aatttcagca ttccagatgc tcagggcggc gctttcgggg gaataccagg
    12001 gggcaccggt ttcgatgacc gatgaagtaa aagaaatggt gagcgagctg atcaacgttg
    12061 gagaagtcaa gctgcccgaa ggacttcagg cgttgatgcg cccctaccag cacaggggtt
    12121 tttcatggat ctaccgcaat gcacagatcg gtttcggatc ggtgcttgcc gatgacatgg
    12181 ggttggggaa aaccctccag gtcatagcca ccatcctcaa attcaaggaa gaaagagttc
    12241 tcgagaagga aaaggtactc gtggtggctc ccaccgggtt actaaccaac tggcaggctg
    12301 aaatccagaa atttgcacca tccctgctga cggaaattta tcacggcacc acacgcgacc
    12361 tgaaacggct gagtgaggtt gacatcctga tcacctccta cgggatggcc cggagggatg
    12421 ccgatcaatt taaaaagatg aagtggcata ccctggttat cgacgaagcc cagtacataa
    12481 aaaaccagga aacccaacaa accaaagcca tcaaatccat caaagcgggg aattgtattg
    12541 ccctgagcgg cacgccggtg gaaaaccggc tgagcgaact ttggagcatc atggacttta
    12601 gcaaccgcgg attcctggga tccctcaaag aatttcagga tacctgggca caaccgattg
    12661 aacaactcaa cgaccgcgaa acagcaaaca aactgaagaa agtcactgcc ccgtttatga
    12721 tgcggcgcct gaaaaccgat aaaaacatca tctcagacct gcccgacaaa atcgagatcg
    12781 acagctacgc gtcattgacc agtgtgcagg ccggactcta tgaaaaaacc ctcgatgaag
    12841 ccatggacgc cattgaaggg gtttccggca ccgaccagaa agcgctcttc gtaaggcagg
    12901 gactggtctt gcagatgatc ctcgccttga aacagatttg caaccacccc gcccaattcc
    12961 tcaaaaacgg ggagacagac gcttcgctga gcggaaaggt ggaactgtta ttcgacaaac
    13021 tcgacagcat cacagagagt aacgagaaag tcctgctttt tacccaattt acccagatgg
    13081 gtgacctgct ccgtcacttc atcacagaac ggtaccacaa cgaaccgctt tatattcacg
    13141 ggggatgttc gatcgaccaa cggaaggaga tggtagagcg gttccagaac aaccacgccg
    13201 accgcatttt cctgttgtca ctgaaggccg caggtaccgg actgaatttg acagccgcca
    13261 gccatgtaat ccattacgat ctctggtgga atccggcggt ggaagcccag gccaccgacc
    13321 gggcctaccg gatcggtcag aaaaacaatg tgatggtcca ccgcttcatt acccggaaca
    13381 cctttgaaga aaagatcaac gaaatgatcc ggaagaaaaa ggcattggcg gaaatgaccg
    13441 tggccaccgg ggaaagctgg atcggcaacc tcagcaacaa ggaactgcgg gaaatcttta
    13501 cgccggggga aaaatgacgg attattcttt ggggcattat tgtgatataa atgtatattt
    13561 gtatcatgac tccgaagaaa ttaccatacg ctccattaca agaagttata tttgagggta
    13621 aatgggaact ttcaattgat cctcatacta aaacatttgt tgatcctgaa tacccgtttg
    13681 ccttggggaa atttcaacaa tccataaaca gcaaatttcc tgtttacaaa gtgaaaatcc
    13741 ctggtggtat accttttcaa atgcttagcc atcaacctgt tcaccagttc tggagtaaag
    13801 atgggatttg gccggttatt cagtatggtc ctggagttgt aacattaaat gacactgaaa
    13861 aaaattatac atgggaagat acttttttcc ctttggcaaa agatacatta ttaaacatca
    13921 aaaatgcata ttctactgag ttggccttta tttcatatac attacgctat attgacgttg
    13981 taaacataaa aaactataag ttcaccaact ggatggattt cgttaaagag aatataaatg
    14041 ttgagtttaa taacaatttc attcctcctg gtccattgag ccagtttaat tttgaacaaa
    14101 catttgaaat caacgaggtg ggaacgcttg aaattagttt ttcaaatgga caagaccata
    14161 aaaatgagga aatattcatc tggcaaaatg cggtttctgg aaaaaatgcg ttgacttgtg
    14221 aagaattggt ttcctggctg agcaaagctc atggattttg ttcaaacatt tttaaagaaa
    14281 tttgtaaaaa ggatttttat gatagtttta ttagccgata aacctgagca agcctcaatg
    14341 agtaccattc ttaaaccaga taatgaagaa atatttccta aatggtttgt aaaaagaagg
    14401 aactacgttg ctccgaatag tacggttttt ccggatatta ctgttttttc aggagatata
    14461 atcgatttat tacaaaagct aaacagcttt acaagcttaa aatataattg ggattcttat
    14521 ggtgctattc ctccatcatt aatgtcaata agaaatgcca gacgtttttt attcaaaaac
    14581 gcgaacgttt ctctaccttt ttattttgta tctcccggaa ttaatggtga agtaatgatc
    14641 gaattgaaca aggatcaaaa agctgccgaa atatacttca atcctgatgg ttccgaagaa
    14701 cttctgcttt acgaaaacaa caattgtatt ctggaaaaag aggatgtgag ttcatacacg
    14761 gatcttatag aatttttcaa tgagtgaatc tttgccacaa ataaccattg aagataatga
    14821 ccgtctgttg agaagagtcc tgtttcttca ccctgatttc ataaaacctg acggcacacc
    14881 ctcctcttca agtttttctt taaaaacagg agaaaaaggt ctttctgttg acatcgaacg
    14941 actgacaacc tatgacaggt ctattttaga taaaagcaga ttcagacttt attttcttaa
    15001 ggcttctttt acaactcaac ttggtttgac aaaccaacac gatcctttac ccgacaatta
    15061 tgcccatgct ttaattaccg gaaacttttc cagatcaatt tgcagattgc tcgcttcttt
    15121 cgccaaacgg ataaactttc cataacgttt ttcagaaagt taaatgcact cgtcagtaat
    15181 ttgtccggca atgataaacg gcaatgataa acaaataggt gcttccttca tggcatcata
    15241 acggccggta agatgtttgt caaaatcgtt tatggaaaac tataatcata caaaaagatc
    15301 tgccattttt acttcgctct gaaccatgcc tgcatattca ttttgtttta ctccaaaggt
    15361 agtaatgaaa gttaagaata cagatttgga agtttgggtc tcttctttaa aagtacctat
    15421 tttatttcgc aggtttgctg catatttttt atcaatggca aatggatgga tagagtactt
    15481 catctcacaa agattgatca ccgaatcgtt tctgtcgatc agcaggtcaa tttgagctcc
    15541 gtttcccgtt cctttacttc gccatgaggc atatctgcag tgaactccag agattccaag
    15601 actttgcctg atttgctgaa tatggacaac gcatacccgt tcaaatgcat aaccactcca
    15661 gatccggtgc tgcggagtgt caatcattgt actccagtga ttctcactgc ttgcaaaatt
    15721 cagattcaga aaatgataat aaaacaaagt aaagaaatcg gttacctgaa aaattttatc
    15781 ccgtgatttt tttccaaaag aaaggtaact tcgaataaaa ccacaatttt ccagatcttc
    15841 caacattttg gacaagcctc ctcccgtttt aaccttacct gatttaatca gttccccacg
    15901 ggttaatcct ttgcttttcc ggcttatcgt ttccatcagt tgaacatatt tatccgaatt
    15961 tctgaataag gcagcatata aatttccaaa ttcattcctt aatgccccac tttcatggaa
    16021 aaacaggtaa tccacatttt gggccaggct gagtcctttt cttaagagac tccagtaata
    16081 gggtatcccc ccaaaaatca tgtaacattc tgttatctgg tattcctccc agcttatttc
    16141 gactgattgc agaaattgtt tgcattccag caaggtgaac ggccgtaggg gtatcctctg
    16201 cgtgatccga ttgtgtaatc ctcttttgtt attcaaaagc ttattcatca tccatgaagt
    16261 agcagatcca caaacaatta atacgatgtc cgatctgccc gatgcccatc cattccagaa
    16321 atgctccagg gaactcataa aatccgattt gggagtatcc atccagggca attcatccag
    16381 aaaaaccact tttcgcttat tccgggattt ttccagcaat tcaatgagtt gttgaaaagc
    16441 acaaagccag tttttaacaa ccggagcatg ggtaagtcct gcccttcgca tcgaaaactg
    16501 aaaattgaga agttgagaag ccaaattggc attggcaaga cccgttagat aaaaatcaaa
    16561 agtattgttg aaaaactccc ggatcaaaaa agtctttcct accctccgcc tgccataaac
    16621 agcaataaac tccgaggtat ccgactgata atacctttgt aattcattct gctctttcaa
    16681 acgacctata attttcgtca taacgccggc atttataatt cgccataaat atacaaaaaa
    16741 atacacttat ggctaattat agatctatat ttcgccataa ctcctgtaaa aacagagata
    16801 tggcgaaata tattgaattc aggaggatct tgtcactcat t
//
LOCUS       DAOPMW010000031        16824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3333_length_16824_cov_8.955096, whole genome shotgun sequence.
ACCESSION   DAOPMW010000031 DAOPMW010000000
VERSION     DAOPMW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16824
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3333_length_16824_cov_8.955096"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLU11_02970"
     CDS             <1..904
                     /locus_tag="PLU11_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HPN58101.1"
                     /translation="AGEQKWKIDSTVTHIDTRSVEREDSVMSLPLIEKPNNPFEGSKD
                     FAGRKNILITEWGPYDFRSPIIWNTNATDTSGLMQFDILGPAGQWKIRKYYGVEGVSA
                     VNGEVPAKITARKLNGERTDILIELEYTGQEVHTPFGEIVKAGKPYRFQFRRFFQPIR
                     WEVNWFTYDSLTNPVRFPERLVRLDQQKPVKSEIADKLDYAWWGGIRVGEKQIQQFIT
                     LANGQANIPDGEYELSVTWDDAVRVYLDGKIILDEWEPSRYSFDESPNRKIKLHLSGN
                     HNFKVEHAELNGFATLAIKLRKLD"
     gene            1021..1326
                     /gene="rpsJ"
                     /locus_tag="PLU11_02975"
     CDS             1021..1326
                     /gene="rpsJ"
                     /locus_tag="PLU11_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPN58102.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAEKIVKTVRSTGAVVTGPIPLPTR
                     RKIFTVLRSPHVNKKSREQFQLATHKRLLDIYTSSSRTVDALSKLDLPSGVEVEIKA"
     assembly_gap    1736..1835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1930..2565
                     /gene="rplD"
                     /locus_tag="PLU11_02980"
     CDS             1930..2565
                     /gene="rplD"
                     /locus_tag="PLU11_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPN58103.1"
                     /translation="MKVEVLNTKGTATGRSVELPEEIFGAEPNNHVIYLAVKQYLAAQ
                     RQGTHKVKTRAEVQGASRKLHRQKGTGGSRKGNIRNPLYKGGGTIFGPKPHSYDFKLN
                     RKVKDLAKISALSYKAKENAIMVVEDINMDSPKTKSFMDIMGSLKVADKKVMFVQPEY
                     NDNVELSLRNVPSVLGMLLSDINTYDIVNSEVLILTESAAKIFADDEKAEA"
     gene            2670..2969
                     /gene="rplW"
                     /locus_tag="PLU11_02985"
     CDS             2670..2969
                     /gene="rplW"
                     /locus_tag="PLU11_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPN58104.1"
                     /translation="MKLSEVLIKPILTEKANAQQDKLRRYAFKVARKANKLEIKKAVE
                     TFYGVTVTGVNTAVVPGKSKSRFTKSGVISGRKPAYKKAFVTVAEGENIDLYSNI"
     gene            3068..3904
                     /gene="rplB"
                     /locus_tag="PLU11_02990"
     CDS             3068..3904
                     /gene="rplB"
                     /locus_tag="PLU11_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPN58105.1"
                     /translation="MALRKFKPVTAGTRWRIGNAYAEVTTNVPEKTLVEAKPRTGGRN
                     SSGHLSMRYRGGGHKKKYRIIDFKRNKEGVATVESIQYDPNRTAFIALLNYADGEKRY
                     ILAPQGLTVGAVVESGATVAPEVGNALPMKNMPLGTNIHNIEMQPGQGGKLARSAGSS
                     AQLTNKEEKYAILKMPSGELRKVLINCWATVGVVSNSDHSLQSMGKAGRNRWKGIKPR
                     NRGVAMNPVDHPMGGGEGKASGGQPRSRNGQYSRGLKTRTKGKGSDKMIIQRKNGNKL
                     AK"
     gene            3937..4206
                     /gene="rpsS"
                     /locus_tag="PLU11_02995"
     CDS             3937..4206
                     /gene="rpsS"
                     /locus_tag="PLU11_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPN58106.1"
                     /translation="MARSIKKGPYVATHLEKKVDAINEGKNKKGVIKTWSRRSTITPD
                     FVGHTFAVHNGNKFIPVYVTEFMVGHKLGEFAPTRQFKGHSKKEG"
     gene            4224..4568
                     /gene="rplV"
                     /locus_tag="PLU11_03000"
     CDS             4224..4568
                     /gene="rplV"
                     /locus_tag="PLU11_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPN58107.1"
                     /translation="MEAVAKLRNYPTSPRKMRLLADLIRGQKVEKVLAELEHNPKHPA
                     VPLRKLVLSAISNWKQKNEGGDESQLVVKTIFVDGARTLKRMRPAPQGRGYRVRKRSN
                     HVTVIVDTKETK"
     gene            4599..5423
                     /gene="rpsC"
                     /locus_tag="PLU11_03005"
     CDS             4599..5423
                     /gene="rpsC"
                     /locus_tag="PLU11_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPN58108.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYGSKKDYAGKLIEDHRIRTYLN
                     ARINKGGISKIVIERTLGKLIITIHTSKPGIIIGKGGGEVDRIKEELKKLTGKDDVQI
                     NILEIRRPELDAMIVGDTIARQIENRINFKRATKMAIASALRMGAEGIKIKLSGRLAG
                     AEIARSEEFKQGRVPLHTFRMDIDYANVFAQTVYGKIGIKVWICKGEVLAKRDLNPNF
                     VGGKSDVSDRRDGRGEHGGGGDRRDHRGGDRRDDRRGGGGGGDRRGGGGRGGERRN"
     gene            5549..5971
                     /gene="rplP"
                     /locus_tag="PLU11_03010"
     CDS             5549..5971
                     /gene="rplP"
                     /locus_tag="PLU11_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPN58109.1"
                     /translation="MLQPKRTKHRKMQKGRMKGDAKRGTTIAFGSYALKALDQHWITD
                     RQIEAARQALTREMKREGNVWIRIFPDKPITRKPQEVRMGKGKGNLEFWAAVVKPGRI
                     MFEIDGVSEQVARASLALAAGKLPIKTKFIVRRDLVTA"
     gene            5993..6202
                     /gene="rpmC"
                     /locus_tag="PLU11_03015"
     CDS             5993..6202
                     /gene="rpmC"
                     /locus_tag="PLU11_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPN58110.1"
                     /translation="MSKKAEFVKSIHGMNEQDLKARLEEDKQRLKKLEFAHAISPLEN
                     PMTIRGLRREIARLQTELKLKQQQA"
     gene            6270..6527
                     /gene="rpsQ"
                     /locus_tag="PLU11_03020"
     CDS             6270..6527
                     /gene="rpsQ"
                     /locus_tag="PLU11_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPN58111.1"
                     /translation="MEARNLRKTRIGVVTSNKMAKTITVAVERKVKHPIYGKFVKKTT
                     KFHAHDDKNECSIGDLVKIMETRPLSKTKRWRLVEVVEKVK"
     gene            6638..7006
                     /gene="rplN"
                     /locus_tag="PLU11_03025"
     CDS             6638..7006
                     /gene="rplN"
                     /locus_tag="PLU11_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPN58112.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQRYARIGDKIVVTVK
                     DAIPQGGIKKGTVAKAVIVRTTNKLRRKDGSYIRFDDNAVVILNQSDEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            7043..7378
                     /gene="rplX"
                     /locus_tag="PLU11_03030"
     CDS             7043..7378
                     /gene="rplX"
                     /locus_tag="PLU11_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPN58113.1"
                     /translation="MKARFKPKYNIRKGDSVVVITGDDKDLSKPRTVKEVLVEKGKVI
                     VEGVNIITKHTKPSAQNTKGGIVKKEAPIAISNVMLWDAKAKAPARITRKREEGKTVR
                     ISKKSGEKI"
     gene            7408..7983
                     /gene="rplE"
                     /locus_tag="PLU11_03035"
     CDS             7408..7983
                     /gene="rplE"
                     /locus_tag="PLU11_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPN58114.1"
                     /translation="MSTKTYAPRLADKYKKDVVPALVKKFSYATVMQAPKLEKICLNR
                     GVNGAVSDKKMVDIAIEELTTISGQKAVATMSKKDISNFKLRKNMPIGARVTLRGDKM
                     FEFLDRLISVALPRVRDFKGVNEKAFDGRGNYTLGVTEQIIFPEIDIDKVSKITGMDI
                     TFVTTANTDEEAYELLKELGMPFKNAKNNSN"
     gene            7998..8267
                     /gene="rpsN"
                     /locus_tag="PLU11_03040"
     CDS             7998..8267
                     /gene="rpsN"
                     /locus_tag="PLU11_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPN58115.1"
                     /translation="MAKTSVKARQRKRERMVAQFAAKREELKKSGDLKALDELPKNSS
                     KVRLKNRCQLTGRPKGYVRYFGISRVALRDMALNGKIPGLKKASW"
     gene            8342..8740
                     /gene="rpsH"
                     /locus_tag="PLU11_03045"
     CDS             8342..8740
                     /gene="rpsH"
                     /locus_tag="PLU11_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPN58116.1"
                     /translation="MVTDPIADFLTRIRNAQMAGHRVVDIPASNLKKRMTEILYNQGY
                     ILKYKFEDDNKQGVIKIALKYDPATKQPAIQSMERVSRPGLRQYSKPADFRRVKNGLG
                     VAILSTSKGVMTDKDAKSQNVGGEVLCYIY"
     gene            8829..9383
                     /gene="rplF"
                     /locus_tag="PLU11_03050"
     CDS             8829..9383
                     /gene="rplF"
                     /locus_tag="PLU11_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPN58117.1"
                     /translation="MSRIGKKSITIPEGVSITVGKDNVITVKGKQGELKQAIDRDITV
                     EIKDGHVHFVRPTDQIRHRAMHGLYRALIANLVKGVTEGYVRKLELIGVGFKAANQGN
                     VLDLALGYSHNIIFEIPKELKVGTAQEKGQNPIITLEGIDKQLIGQVAAKLRSLRKPE
                     PYKGKGVRYVGEVVRKKAGKAAGK"
     gene            9435..9794
                     /gene="rplR"
                     /locus_tag="PLU11_03055"
     CDS             9435..9794
                     /gene="rplR"
                     /locus_tag="PLU11_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPN58118.1"
                     /translation="MNPKVKSQRRKNIRYRIRRKISGTTAKPRLSVYRSNAEIYAQLI
                     DDTTGQTLAAASSRDKDIVAQKVTKSEKGKLVGAAIARKATELGLKDVTFDRGGYLYH
                     GRVKSVADGAREGGLLF"
     gene            9828..10346
                     /gene="rpsE"
                     /locus_tag="PLU11_03060"
     CDS             9828..10346
                     /gene="rpsE"
                     /locus_tag="PLU11_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPN58119.1"
                     /translation="MSKFNVNRVKAGDLELKDKVVAINRVVKTTKGGRAFSFSALVVV
                     GNENGVVGHGLGKAKEVQEAITKGIEDAKKNLIKVPVMKGTIPHDQWAKEGAAKVLIK
                     PAAHGTGVIAGGSMRAVLEAAGVTDVLAKSLGSANPHNVVKATFKALAMLREPIHVAK
                     TRTMGLKKVFNG"
     gene            10412..10594
                     /gene="rpmD"
                     /locus_tag="PLU11_03065"
     CDS             10412..10594
                     /gene="rpmD"
                     /locus_tag="PLU11_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPN58120.1"
                     /translation="MKKIKVTLVKSPIDRPERQKLTLQALGLNKTNSSREMIATPQIL
                     GMVHKVTHLVKVEEVN"
     gene            10670..11119
                     /gene="rplO"
                     /locus_tag="PLU11_03070"
     CDS             10670..11119
                     /gene="rplO"
                     /locus_tag="PLU11_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPN58121.1"
                     /translation="MKLHELRPAKGATHKTKRIGRGDGSGHGGTSTRGTKGAQARTGF
                     KNKMAHEGGQMPIQRRIPKRGFKNPHRIEYKVINLGQVEQLAEKYNITEFTLENLYIN
                     GLIAQTDRVKVLGNGELKEKFTFRVNAVSDKAKAAIEAAGGSVEIVK"
     gene            11189..12526
                     /gene="secY"
                     /locus_tag="PLU11_03075"
     CDS             11189..12526
                     /gene="secY"
                     /locus_tag="PLU11_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPN58122.1"
                     /translation="MKKFIQTLKNIWSIEELRGKILFTMMMIAIYRLGAHIVLPGINP
                     NKMSETGDSNTGILGLIDAFSGGAFNQASIFALGIMPYISASIFMQLMTVLVPRFQKM
                     QKEGESGRKKINQYTRYLTVIVTILQASAYVTYLQGMTQQNGAIMAGYAGFFWLSTVV
                     VLTAGTMFVMWMGEKITDKGLGNGTSLIIMIGILARLPQSFLQELSAKSVRNGLILVF
                     IVEIAILIAIIMGCILLIQGVRKVPVNYAKQIVGNRQFGGARQFLPLKVNSAGVMPII
                     FAQAIIFIPTIFANYDTQASGFLRAITDHSNVWYMLIYSVVVIGFTFLYTALIFNPKQ
                     IADNLKQNNGFIPGVKPGQPTADYIGSIMDKITFPGAILLAFVGILPGIAERLGVTQQ
                     FSTFFGGTSLLIMVGVILDTLQQIETQLLMRQYDGLMKSGRIQGRQTTSAVQM"
     gene            12597..13394
                     /gene="map"
                     /locus_tag="PLU11_03080"
     CDS             12597..13394
                     /gene="map"
                     /locus_tag="PLU11_03080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPN58123.1"
                     /translation="MILYKTDEQVEQMRKSALLVSHTLTQVAAMLKPGLDTLSIDQLI
                     GTYIRDHQAIPSFLNYHGYPFNSCISVNDVVVHGFPNKRELKDGDIITVDVGVILNGW
                     HGDHAYTFAIGDPGKDVMKLIQVTKESLYKGIEKAVAGNRVGDIGFAIQDYTEKKHGY
                     GVVRELVGHGLGKDLHEDPQVPNYGKRGSGAKLKEGMVLAIEPMINMGKKEVYTEEDG
                     WTVRTRDGKPSVHFEHDVCIRKDKADVLSDYGPIEKAEALNSNLFTY"
     gene            complement(13546..14136)
                     /locus_tag="PLU11_03085"
     CDS             complement(13546..14136)
                     /locus_tag="PLU11_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPN58124.1"
                     /translation="MAGQLTKKELQEIPVVPYDKIRDYLQTGHIFFSSGSYTFSGMIQ
                     RLTKSVWSHVAIVYKDEKLGRVLVLEAEPRVGIRMIPLSNYLRNYQGKKRPYKGQVVI
                     ARLNSQLEEQQLNKAISFGLDQLTRPYDNWEIIRIMLRILFRKGKRERNKSYICSELV
                     RDAYAKSGVLFKMNDTYISPQEIWKDERVEIKYRLL"
     gene            14200..15426
                     /locus_tag="PLU11_03090"
     CDS             14200..15426
                     /locus_tag="PLU11_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN58125.1"
                     /translation="MAKKLVVIGGGAAGIFCAVNAARMNPVLDVILLEKSSKFLSKVR
                     ISGGGRCNLTHACFDREEMSRHYPRGQRFVKKAFHRFFTTDTIQWFEERGVPLKTEAD
                     GRMFPQSDNSESIVSCLLKEVNQYKVDIRMNAEVLQLQEKENGFALLLSQAKTLEADY
                     VCVACGGYPKTSMFDWLLQTGHTIEPPVPSLFTFNIPHHPVTGLMGVSVPAATVKISG
                     TKMEETAPLLITHWGLSGPAVLKLSARGARELAERNYHFSVIVNWLPGQQDQQLAIQF
                     RSIRTELAAQKISLRNPFGLPQRLWDLLLELSGIDKEKRWADLPAKEQNKLVANIVHC
                     SFDVKGKTTFKEEFVTAGGISLSGIDVNTMQSKTVPGLYFAGEIMDVDGVTGGFNFQH
                     AWTSGFIAASAVAGRE"
     gene            complement(15625..16593)
                     /locus_tag="PLU11_03095"
     CDS             complement(15625..16593)
                     /locus_tag="PLU11_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPN58126.1"
                     /translation="MLYHYLKLMAGIAIRVFFRKIVINHPERLREKGPLLLASNHPNS
                     FLDSVLLNTIFRQPVWSLARGDVFKKPLFRKLLTAVKILPVYRTSEGVENLSENYKTF
                     DACMELFKQNGIITIYSEGKCINEWHLRPLKKGTARLAIKAWEENIPLRVLPVALNYS
                     SFTRFGKNIFINFGEIITSDVIAAHTADGPRNLEFNNRLRQELEKGVYEIPSTDIKQR
                     KEKLSVPVSSLKKIILFLPGCLGWLINVPPFIFARKNAISRLKEPGHYDSVVMGILLL
                     TYPLYILLLTITLLLISHSWWSFLFPIVALFTAWAYVQVKAQMSNE"
BASE COUNT         4993 a         3622 c         4179 g         3930 t
ORIGIN      
        1 ggccggtgaa caaaaatgga aaatagattc cactgttact catattgata cccgttctgt
       61 agagcgggag gattcggtca tgagtctgcc tttgatagaa aaaccaaaca atccttttga
      121 aggaagtaag gattttgccg ggcgtaaaaa catactgatc accgaatggg gaccctatga
      181 tttccgcagc cccattatct ggaataccaa cgcaacggat acatccggac tgatgcagtt
      241 tgatatactg ggtccggctg gacaatggaa gatcaggaaa tattacggtg tggagggagt
      301 atccgctgta aacggggagg ttcccgcaaa aatcactgcc cggaaactga acggtgagcg
      361 gacggatatt ctgatcgaac tggaatatac cgggcaggag gtgcatacgc ctttcgggga
      421 gatagtgaaa gccgggaagc cttatcgctt tcagttccgc cggtttttcc agcccatccg
      481 ttgggaggta aactggttca cttatgattc actgaccaat ccggtcaggt ttccggaacg
      541 attggtgcgg ctggatcagc aaaaacctgt aaagtccgaa attgccgata aactggacta
      601 tgcctggtgg ggtgggatca gggtggggga gaagcagatt cagcaattta tcaccctggc
      661 aaacgggcag gcaaatatcc cggatgggga gtatgagctg tcagtaacct gggatgacgc
      721 tgtaagagtg tacctggatg gcaaaataat cctggacgaa tgggagcctt cccggtacag
      781 ttttgatgag tcgcccaatc gaaaaataaa attgcatcta tctggaaatc ataattttaa
      841 ggtcgagcat gctgagttaa acggctttgc caccctggcg ataaagctcc ggaaactgga
      901 ttaatccaca gctggggaaa agagtttctc cacagggcct aaaattcttt cataagtatt
      961 tggaaggtat agccttcgcc cttacctttg cactcccaaa agaaaattgg gtttttacac
     1021 atgtctcagc gaatcagaat caaattacag tcgtacgatc acaacctggt agataaatct
     1081 gcagaaaaga tcgtcaaaac ggtacgtagt accggagccg tggtaacagg tccaattccc
     1141 ctgcctaccc gccggaagat atttacggta ttgcgttccc cgcacgtaaa taaaaagagt
     1201 cgtgagcagt tccagctggc tacgcataag cgtctgctgg atatttatac ttcatccagc
     1261 cgtacggtgg atgccctgag caagcttgac ctgcccagtg gtgtggaagt agagatcaaa
     1321 gcgtagtacc ctgcgtgctg tgctgatcga atctagttct tatattttgt atttaagtaa
     1381 ttgctactct ctcaactggt aatccataag caaaccgaag ggccctcctt cgctgaagct
     1441 acggacggcc aggaaaagag ctctggctta taaacataaa acaagccatt cagggtttgt
     1501 ttaccgctgg tacttcccca taaaggaaaa ggtcggtggc gaacggggat tgacccttcg
     1561 aaaagtcagg tgacacgccg tctcctgata ctccgaagaa tgtcccaata accttgaggc
     1621 ataaaatcac aaacatgaaa ggtattattg ggaaaaaaat tgggatgacc agcatcttcg
     1681 atccctcagg taaacagacg gcctgtacga tcattgaggc cggtccctgt gtagtnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtacc gggtcatatc ggttcaattg
     1861 tattaattca gaaataattc attacccgcc gggggtgatt atcccgggtg agtcaataaa
     1921 taccagaaaa tgaaagtcga agttttaaat acgaaaggta cagcaacagg cagatcagtg
     1981 gaactgccgg aggagatctt cggagcagag cctaacaatc acgtaatcta cctggctgtt
     2041 aaacaatacc tcgccgccca gcgtcagggt acacacaaag taaagacccg tgctgaagta
     2101 cagggtgcca gccgtaaact gcaccgtcag aaaggtactg gtggaagccg taaaggtaat
     2161 atccgtaacc ctttatacaa gggtggtggt accatcttcg gtcccaagcc gcactcttat
     2221 gatttcaaac tgaaccgtaa ggtaaaagac ctggccaaga tttctgccct tagctacaaa
     2281 gccaaagaga atgcgatcat ggtggtggaa gatatcaata tggattcccc gaagaccaag
     2341 agctttatgg acatcatggg cagcctgaaa gtagccgata agaaagtaat gtttgtgcag
     2401 ccggaatata atgacaatgt ggaactgtcc ctgcgcaatg tgccttctgt actcggtatg
     2461 ctcctgagtg atatcaatac ctatgatatt gttaactcag aagtgctgat cctgacagaa
     2521 agtgcagcga agatctttgc tgacgacgag aaggctgaag cgtaattcat ctttcgggcg
     2581 gtgtcttcgg acaggctcag gatgacatcc ttcggcaggc tcaggatgac aaaaaaagag
     2641 cttattacaa acaattaaat tgaattcaga tgaaactttc tgaagtactg ataaaaccga
     2701 tcttaactga aaaagcaaac gcccagcagg acaaactgcg tcgctatgct tttaaagtag
     2761 cccgtaaagc aaataagctg gagatcaaga aagcggtaga aaccttttac ggggtgactg
     2821 taacaggagt gaataccgct gtggttcctg gtaaaagcaa aagccgtttc accaaatccg
     2881 gagtgatctc tggtcgtaaa ccggcgtata aaaaagcttt tgtaacggtg gcagaagggg
     2941 agaatatcga tttgtattcg aatatttaat ccgggaagat cctccttcgc caaggctacg
     3001 gaggataata atggcaaaca accgccgcga agtgttggaa taagaaatta taaaagagaa
     3061 ttttaaaatg gcactaagaa aatttaaacc cgttacagcc ggtacccgtt ggagaattgg
     3121 taacgcttat gctgaagtta ctaccaatgt acctgaaaag accctggtgg aagccaagcc
     3181 ccgcacgggc ggacgtaact cttccggtca cctgtctatg cgttacaggg gtggcggtca
     3241 caagaagaaa tatcgtatca tcgatttcaa aagaaataaa gaaggggtag ctacagttga
     3301 atctattcag tatgacccga accgtacagc cttcatcgcc ctgctgaatt atgcggatgg
     3361 cgagaagcgt tatatcctgg ctccccaggg gctgactgta ggtgcagtag ttgaaagtgg
     3421 tgctactgtt gctcccgaag ttggtaatgc gcttccgatg aagaacatgc cgcttggtac
     3481 caacattcac aatattgaaa tgcaacccgg tcagggtggc aagctggccc gcagtgcagg
     3541 atcttctgcg cagctgacca acaaagaaga aaaatatgcg attctcaaaa tgccttccgg
     3601 tgaattgaga aaagtactga tcaattgctg ggcaacagta ggggtggtga gcaacagcga
     3661 ccatagcctc cagagcatgg gtaaagccgg tcgtaaccgc tggaagggta tcaaaccccg
     3721 taaccgtggt gtggctatga accctgtaga tcacccgatg ggtggtggtg aaggtaaagc
     3781 ttcaggtggt cagccccgca gcagaaacgg ccagtactcc agaggtctga agacgagaac
     3841 caaaggaaaa ggcagtgata aaatgatcat ccagcgtaag aacggaaata agctggccaa
     3901 gtaatcaaac tcattaacaa ctcaacgaca taaatcatgg ctcgttcgat taaaaaaggt
     3961 ccttatgtag cgactcacct tgaaaaaaag gttgatgcta tcaatgaagg gaaaaacaag
     4021 aagggtgtta tcaagacctg gagccgccgc tccacgatca cacctgactt tgtgggtcac
     4081 acgttcgctg tgcacaacgg caacaagttc atccctgttt atgtaaccga atttatggtt
     4141 ggacataaac tgggagaatt cgcccccacc cgtcagttca aaggacactc taaaaaagaa
     4201 ggataaacaa gaaattaatt acaatggaag cagtagctaa actgagaaat tatcccacct
     4261 cgccgcgcaa aatgcgtttg ctggctgacc tcatccgtgg tcagaaagtg gagaaagtac
     4321 tggctgaact ggagcacaat cccaagcacc ccgcagttcc cctgcgcaag ctggtgctga
     4381 gtgccatcag caactggaag cagaagaatg agggtggtga tgaaagccag ctggtggtaa
     4441 aaaccatctt cgtggacgga gcccgcacct taaagagaat gcgtcccgcg ccccagggcc
     4501 gtggttaccg tgttcgcaaa cgcagcaacc atgttactgt gatcgtggac acaaaagaaa
     4561 ctaaataatt aatcgactaa cgcataacct gaataaacat gggtcaaaaa gcaaatccga
     4621 ttggtaacag gttaggtatc atccgcggat gggaatctaa ctggtacggc agcaagaaag
     4681 actatgctgg taaactaatc gaagatcacc ggatcagaac ttatctgaat gcacgtatca
     4741 acaaaggcgg tatctccaag attgttattg agcgcaccct gggtaaactg atcattacca
     4801 ttcatacatc caagcctggt attattatcg gtaagggtgg tggagaagtg gatcgcatca
     4861 aggaagagct gaagaagctg accggtaaag atgatgtaca gatcaacatt cttgaaatac
     4921 gtcgtccgga actggatgcg atgatcgtgg gtgatactat tgcccgtcag atcgaaaacc
     4981 ggatcaattt caaacgggcc actaaaatgg cgattgcttc tgccctgcgt atgggtgcgg
     5041 aaggaatcaa gatcaaactg agcggccgtc tggctggtgc agagatcgcc cgtagcgagg
     5101 aattcaaaca aggacgtgtg cccctgcata cattccgtat ggatattgat tatgcaaacg
     5161 tgtttgccca gaccgtatat ggtaaaatcg gtatcaaagt atggatctgt aaaggtgaag
     5221 tactggccaa gcgtgacctg aaccccaact tcgtaggtgg aaagagtgat gtaagcgacc
     5281 gcagagatgg ccgtggcgaa catggtggcg gcggtgatcg tcgtgatcat cgtggtggcg
     5341 accgtcggga tgaccgcaga ggcggtggtg gtggtggtga ccgcagaggt ggcggcggaa
     5401 gaggtggcga acgcaggaat taagaaagca aggagcagcg agtcacgacg agttacgagc
     5461 tgcggaccgt ttcaaataaa ataaagagtt taattgcttt agggtagggg ctcgaaacct
     5521 gttactcaaa gctcaaagct taaaagaaat gttacaaccg aaaagaacga agcacaggaa
     5581 aatgcagaag ggccgcatga aaggcgacgc caaaagaggt actactattg catttggttc
     5641 ttatgcttta aaagcactgg atcagcactg gattactgac cgtcagattg aggcggcccg
     5701 tcaggcgctt acccgtgaaa tgaaaaggga aggtaatgta tggatccgca ttttcccgga
     5761 taaaccaatt acccgcaaac cccaggaagt aaggatgggt aaaggtaaag gtaacctcga
     5821 attctgggcg gccgtggtta aacccggacg tatcatgttc gaaattgatg gagtaagtga
     5881 gcaggttgcc cgtgcctccc tggccctggc tgccggtaaa ctgccgatca aaaccaaatt
     5941 tattgtgcgc cgtgacctgg ttaccgcata atcattaata aatcgtacag cgatgtcaaa
     6001 gaaagcagaa ttcgtaaaaa gcattcacgg aatgaacgag caggacctga aggcccgtct
     6061 cgaagaagac aaacaacgcc tgaaaaagct ggagtttgcc catgcgattt ctcccctgga
     6121 gaatcccatg accatccgcg gactgcgccg tgaaattgcc cggctccaaa ctgaactgaa
     6181 gttaaagcaa cagcaagcat aattagtctg aagtccggaa aagggagtcg ggaatcgact
     6241 cagacagccg gtcgtctaaa aaaaataaaa tggaagcaag aaatttaaga aaaaccagga
     6301 ttggtgtggt aaccagcaat aaaatggcta aaaccattac ggttgcggta gaaagaaaag
     6361 taaaacaccc tatctatggt aagttcgtaa agaaaaccac caagttccat gcgcatgatg
     6421 acaaaaacga atgcagcatt ggtgatttag tgaaaatcat ggaaacccgc ccgctgagca
     6481 aaacaaagcg ctggagactg gtggaagtgg tggagaaagt caagtaagag aaagatacga
     6541 gctacgagcg ttgagctacg agccgtgcta agaataaaca gattgtatta attgcttgag
     6601 ctcgtaactc gtggctcaaa gcttaaagct taagaaaatg attcagcaag aaagccggtt
     6661 aaatgtagcg gacaacagcg gtgccaaaga ggtgctttgt atccgtgtac tcggtaacag
     6721 cggccagcgc tatgcccgga ttggcgacaa aatcgtagta acggtgaaag acgcgatccc
     6781 ccagggtggt atcaaaaaag gtacagtagc caaagcggtg attgtacgta ccaccaataa
     6841 gcttcgtcgt aaagatggtt cttacatccg ttttgatgac aacgcggtgg tgatcctgaa
     6901 ccagtctgat gagcccaggg gtacccgtat cttcggcccg gtagcccgtg agctccgtga
     6961 taaaggatac atgaagatta tttcactggc tcccgaagta ttataattga taaaagggta
     7021 atacccatta acgcaagata aaatgaaagc aagatttaaa cccaagtata acatccgtaa
     7081 aggcgacagc gtggtggtga tcaccggcga tgataaagat ttatctaaac cccgtacggt
     7141 gaaagaagta ctggtggaga aaggtaaagt gattgtggaa ggtgtgaaca tcattaccaa
     7201 gcacaccaaa ccttctgccc agaatacaaa gggtggtatc gtgaaaaaag aagcacctat
     7261 agcgatcagc aatgtgatgc tgtgggatgc caaagccaag gctcctgccc ggatcaccag
     7321 gaagcgggaa gaaggcaaaa ctgtaagaat ttcaaaaaaa tcaggcgaga aaatctaatt
     7381 tctgacctgg cgtaataagc gaataatatg agtacaaaaa catatgcccc gagactggca
     7441 gacaagtaca aaaaagacgt tgtacctgcc ctggtaaaaa agttcagtta tgccactgtg
     7501 atgcaggctc ctaaactgga gaaaatctgc ctgaaccgtg gtgtaaacgg agcggtgtct
     7561 gacaagaaaa tggtggatat tgccatcgag gaactgacca ccatttccgg tcagaaagcc
     7621 gtggccacca tgtctaagaa agacatctcc aacttcaaac tccgtaagaa catgccgatc
     7681 ggcgcccggg ttaccctgcg tggcgacaaa atgtttgaat tcctggatcg cctgatttcc
     7741 gtggcactgc cccgtgtacg tgacttcaaa ggagtaaatg aaaaagcttt tgacggccgt
     7801 ggtaactata ccctgggtgt gacagagcag atcattttcc cggagatcga tatcgataaa
     7861 gtaagcaaaa tcaccggtat ggatatcacg tttgtgacca ccgccaacac cgatgaagag
     7921 gcttatgagc tgctgaaaga actcggtatg cctttcaaaa acgcaaaaaa taattctaac
     7981 taaaagaata atcactcatg gcaaaaactt ctgtaaaagc caggcagcgg aaaagagaaa
     8041 gaatggttgc acaatttgca gctaaacgcg aagagctgaa aaaatccggc gacctgaaag
     8101 cgctggatga gttaccgaaa aactcttcca aagtccgctt aaaaaaccgt tgtcagctga
     8161 cggggcgtcc taaaggttat gttcgttact ttggtatttc cagggtagcc ctgcgtgaca
     8221 tggcgttgaa tggcaagatc cccggtttga aaaaagcaag ctggtaaaca aaacaaatta
     8281 attaaactaa acttttccaa ttatcccgac gcttcggtcg ggacgtagga gggcaataaa
     8341 tatggtaaca gatcctatag cagatttcct gacaagaatc cggaacgcac aaatggcagg
     8401 tcaccgtgta gtggatattc ccgcttccaa cctgaaaaag cgtatgaccg agatcctgta
     8461 caaccagggt tatatcctta aatacaaatt tgaggatgac aacaagcaag gcgtgattaa
     8521 gatcgccctg aagtatgatc ccgccaccaa gcaacccgct atccagagca tggagcgtgt
     8581 aagccgtccg ggtctccgtc agtactccaa accggctgat ttccgccgcg tgaagaacgg
     8641 actgggtgtg gccatcctgt ctacttccaa aggtgtaatg actgataaag acgccaaatc
     8701 ccagaatgtg ggtggtgaag tactgtgcta catttactaa aactgatgtt gccgatacat
     8761 taagccgaac ctatataggc tgaccggttg gtaacgaaac taatagatca cttaataaaa
     8821 aatattttat gtcccgtata ggtaaaaaat cgatcaccat tcctgaagga gtcagtatca
     8881 ccgtaggaaa agacaatgtg attactgtaa aaggtaagca gggtgagctg aaacaggcta
     8941 tcgaccgtga tatcacggtg gaaattaaag atggtcatgt acatttcgta cgtcctaccg
     9001 accagatccg tcaccgtgcc atgcatggtc tgtaccgcgc cctgatcgcc aacctggtga
     9061 aaggtgtgac agaaggatat gttcgtaaac tggaactgat cggggtgggt tttaaagcgg
     9121 ctaaccaggg taatgtactt gacctggcgc tgggttactc tcacaacatc attttcgaaa
     9181 tccccaagga actgaaagta ggaactgccc aggaaaaagg acagaaccct attatcaccc
     9241 tggaaggaat tgataaacaa ctgatcggcc aggtagccgc caaattacgc agcctgcgta
     9301 aacccgagcc gtacaaaggt aaaggggttc gttatgtggg tgaagtggta cgtaagaaag
     9361 caggtaaagc ggctggtaaa taattaaaca tttaattgca tcctggcagc atcaggatac
     9421 taaatacaac agagatgaat ccaaaagtaa aatcacaaag aagaaagaac atccgttaca
     9481 ggatccgccg caaaatcagc ggaaccactg ccaagccccg tttatcggtg taccgcagta
     9541 acgcggaaat ctacgcccag ctgatcgatg ataccaccgg acagacactg gctgccgctt
     9601 cctccaggga caaggatatc gttgcccaaa aggtaacgaa gagtgagaaa ggtaaactgg
     9661 taggggctgc catagcacgc aaagccactg aactgggact caaggatgta acttttgacc
     9721 gtggtggata cctctatcat ggacgggtaa aatcagtagc cgacggagcc cgcgaagggg
     9781 gtcttctgtt ctaaacaatc cattcaaact tttaattaca agtataaatg tcaaagttta
     9841 acgtaaacag ggtaaaagcc ggtgacctgg aactgaaaga taaagtggta gccatcaacc
     9901 gtgtggttaa aacaaccaag ggcggacgtg cgttcagttt ctctgcctta gtggtggtcg
     9961 gtaacgaaaa cggagtggta ggccacggtc ttggtaaagc caaagaagtt caggaagcaa
    10021 ttaccaaggg gattgaggat gccaaaaaga acctgatcaa ggttcccgtt atgaaaggta
    10081 ccattcctca tgaccagtgg gccaaagaag gtgcagccaa agtgctgatc aagcctgccg
    10141 ctcatggtac cggtgtgatc gccggaggtt ccatgcgtgc cgtgctggaa gcagctggag
    10201 taacagacgt acttgccaaa tcgctgggtt ctgccaatcc gcataacgtg gtgaaagcaa
    10261 cctttaaagc attggctatg ctgcgtgaac ccatccatgt ggctaaaacc cgcaccatgg
    10321 gattaaagaa agtattcaac ggttaaatca taaataaaga atacctgacc ccggcaacag
    10381 ctggcaaaag ggatttgaaa acaggaattt tatgaagaag ataaaagtaa cattggtgaa
    10441 aagtccgatt gacagacccg aacgccagaa gctcacgctg caggcactgg gactgaacaa
    10501 aacgaattct tcccgtgaaa tgattgccac tcctcagatt ctgggcatgg tgcacaaagt
    10561 gacccacctg gtaaaagtgg aagaggtgaa ctaatccgga tttccggatt ctgacaaaca
    10621 aaaatgcgag ccccgacgct tttggggcga tgagtaaaaa ataaaagcaa tgaaattaca
    10681 cgaattaaga cctgcaaaag gcgcaacaca caagacaaaa cgtatcggcc gcggtgatgg
    10741 ttccggacat ggtggaactt caacaagagg tactaaagga gctcaggccc gtacaggttt
    10801 caagaataaa atggcccacg aaggtggcca gatgccgatc cagcgccgta tccccaaacg
    10861 cggtttcaag aatccccatc gtattgaata caaagtaatc aacctcggac aggtggagca
    10921 actggcagaa aaatataata tcactgaatt cacactcgaa aacctgtaca tcaacggact
    10981 gatcgcgcag acagaccgcg tgaaagtgct gggtaatggt gaactgaaag aaaaattcac
    11041 cttccgggta aatgcagtaa gtgataaagc caaagcggcc atcgaggcag caggtggttc
    11101 ggtggaaata gtaaagtaat ttcactaaat tgcacgacgc atttaatgcg tcttttatgt
    11161 tttacgtgct ttaaactttt atatccccgt gaagaaattt atacagacac tcaagaatat
    11221 ctggagcatc gaagagttgc gcggcaagat cctgttcacc atgatgatga tcgcgatcta
    11281 tcgtctgggt gctcacattg ttttaccggg tatcaatccg aataaaatgt cggaaaccgg
    11341 tgacagcaac acaggtatcc ttggattgat cgacgccttc tccggaggag cttttaacca
    11401 ggcttctatc tttgcattag gtatcatgcc ttatatctct gcctctatct ttatgcagct
    11461 gatgacggta ctggtacccc gcttccagaa aatgcagaaa gaaggggaaa gcggacggaa
    11521 aaaaattaat cagtataccc gttacctcac tgtaatcgtt accattttac aagcgtcagc
    11581 gtatgttacc tacctgcagg gtatgaccca gcagaatggt gccatcatgg ccggatatgc
    11641 cggattcttc tggttatcca ccgtagtggt gctgacagcc ggaaccatgt ttgtaatgtg
    11701 gatgggtgaa aaaattacgg ataaaggact tggaaacggt acctccctga ttatcatgat
    11761 cggaatcctg gcccgtctcc cgcaatcatt cctgcaggag ctgagtgcca aatccgttcg
    11821 gaacggtctt atccttgtat ttatcgtgga aatcgccatc ctgatcgcta tcatcatggg
    11881 atgtatcctg ctgatccagg gggtgcgtaa agtaccggta aactatgcca agcaaattgt
    11941 aggaaaccgc cagtttggtg gtgcccgtca gttccttccc ctgaaggtca acagtgcagg
    12001 tgtaatgccg atcatctttg cccaggcgat catttttatt ccgaccattt tcgcgaatta
    12061 cgatacacag gccagcggtt tcttacgggc tatcacggat cacagtaatg tgtggtatat
    12121 gttgatttat tcagtggtgg tgatcggatt tactttcctt tacactgcct tgatctttaa
    12181 ccccaaacag atagctgata acctgaagca aaacaacgga ttcatccccg gtgtgaagcc
    12241 cggtcagccc actgccgatt atatcggatc aatcatggat aaaatcacat ttccgggagc
    12301 tatcctcctg gcatttgtag gtatcctgcc cggtatcgca gaacggttag gtgtaaccca
    12361 gcaattctcc actttcttcg gcggtacttc actgctgatc atggtgggtg taatcctgga
    12421 taccctgcag cagattgaaa cgcaactgct gatgcgccag tacgacggac tgatgaagag
    12481 tggccgtatt cagggccgcc agactacttc tgccgttcag atgtaatacc cgataaactc
    12541 attcaggacc cggtaagacc acttgccggg ttcttttttt taaagatatt tgcaacatga
    12601 ttttgtataa aacagatgag caggtggaac agatgcggaa aagcgccctg ctggtgagtc
    12661 acacgctcac ccaggtagct gccatgctga agcccgggct cgataccctt tctatagatc
    12721 aactcattgg tacctatatc cgggatcatc aggcaatccc ttctttcctg aattatcatg
    12781 gctatccttt taattcctgt atctccgtca acgatgtggt agtgcatgga tttcccaata
    12841 aacgcgaatt aaaagatggc gatatcatca ccgtggatgt aggagtgatt ctgaatggct
    12901 ggcatggtga tcatgcttat acctttgcca tcggagatcc cggtaaagac gtgatgaaac
    12961 tgatccaggt gaccaaggaa tcgctttaca aaggcataga gaaggcagtg gccggtaacc
    13021 gggtgggtga tattggtttt gctattcagg attataccga gaagaaacat ggctatggcg
    13081 tggttcggga actggttggt catggcttag gtaaagacct gcatgaagac ccgcaggtac
    13141 ccaattatgg caaacgggga agcggggcca aattaaaaga aggcatggta ctcgccattg
    13201 aaccgatgat caatatggga aaaaaggaag tgtataccga agaagatggc tggactgtcc
    13261 gcacccgcga cggcaaacct tctgtgcatt ttgaacatga tgtatgcatc cggaaagata
    13321 aggcggatgt tttgtcagat tatggcccta ttgaaaaagc agaagccttg aatagtaatt
    13381 tgtttactta ttgatttttt ttcggtaacc taatttattt tcaaggtacc aggtacgagt
    13441 tttgtgttgc gagagaattt taaaagggag catcagtttg ttaatgaaga actacttact
    13501 taggaggccc tgacaactga cagctgacac ctgacaactt attcctcaca gcagccggta
    13561 cttaatttcc acccgttcat ctttccaaat ctcctgcgga ctgatatagg tgtcattcat
    13621 tttaaagagc acaccggatt tggcatacgc gtcacgtacc agttctgagc agatataact
    13681 tttattccgt tccctttttc ctttccggaa caggatccgt aacatgatac ggatgatctc
    13741 ccagttgtca tagggccggg tcagctgatc gaggccaaag ctgatggctt tattcagctg
    13801 ctgttcttcc agctggctgt tgagccgggc aatcaccacc tgacctttat acggcctttt
    13861 tttaccctgg taattccgga gataattcga tagcgggatc atccggattc ccaccctggg
    13921 ttccgcttcc agtaccagca ctcttcccag tttttcatct ttgtaaacga tggccacatg
    13981 actccataca cttttggtca gccgctgaat catgccggaa aaagtataac tcccggaact
    14041 gaaaaagata tgtccggtct gcagatagtc cctgatttta tcatagggca ccacgggtat
    14101 ttcctggagt tcttttttag taagctgtcc tgccataagc gaattattgt cgtagaacga
    14161 acttaattaa atttcatcgt tctttgcaaa aaacaatacg tggctaaaaa actggtcgtg
    14221 atcggaggag gggctgccgg aattttctgc gccgtgaatg cagcccggat gaatccggtg
    14281 ctggatgtga tcctgctgga gaaatcatca aaatttttat ctaaagtacg gatcagtggt
    14341 ggaggccgtt gcaacctgac ccatgcctgt tttgacaggg aggagatgag ccggcattat
    14401 cccaggggcc agcgttttgt gaagaaggcc tttcatcgtt tttttaccac cgatacaata
    14461 caatggtttg aagaacgggg agtccccctg aaaacggagg ccgatggtcg tatgtttccc
    14521 cagtcggata actcagagag tattgtcagt tgcctgctga aagaagtgaa ccaatacaaa
    14581 gtggatatcc ggatgaatgc ggaagtgctg cagttgcaag aaaaagaaaa tgggtttgcc
    14641 ttactgctca gtcaggctaa aacgctggag gcggattatg tttgtgtggc ttgtggcgga
    14701 tatcctaaaa cgtccatgtt tgattggttg cttcagaccg gtcatactat tgaaccaccg
    14761 gtaccttcac tttttacatt taatattcct catcatccgg taaccggact gatgggggtc
    14821 agtgtgccgg ccgccactgt aaaaataagt ggaacgaaaa tggaagagac ggccccttta
    14881 ctgattaccc attggggatt gagtgggcca gctgtgttaa aactcagtgc ccgcggtgcc
    14941 cgggaattgg cggagcggaa ttaccatttc tctgtaattg taaactggtt gccggggcaa
    15001 caggatcagc aactcgccat acagttccgc agcatacgaa cggaactggc tgctcaaaaa
    15061 atcagtctcc ggaatccttt tggtttacca caacggttat gggatctgtt acttgaatta
    15121 tcaggaattg ataaggagaa acgttgggct gatcttcccg caaaggagca aaataaatta
    15181 gtggcgaata tcgtacactg ttcttttgac gtgaaaggca aaacgacatt caaagaggag
    15241 tttgttacgg ctggcgggat cagtctgagt ggtattgatg taaataccat gcagagtaaa
    15301 acagtacccg gactctattt tgccggggaa atcatggatg tggatggcgt gaccggcggg
    15361 tttaatttcc agcatgcctg gaccagcgga ttcattgcgg cgagcgcggt agctggaaga
    15421 gaataaggaa tgagtaatga ataatgagta atgagtaatg aataatgagt aatgaataat
    15481 gagtagctcg ggaagatact tttgttatag ttgctggcag aaatgcggaa tatttaaaaa
    15541 aaaaataaag atgatgtttt tgtcacgttc aaaaaactgg gtgtttgaac gtttagttca
    15601 attcgatcag ctactcatta ctcattactc attactcatc tgagctttta cctgcacata
    15661 ggcccatgcg gtaaacaaag ccactatagg aaacaaaaag ctccaccagc tatggctgat
    15721 cagcaggagc gtaatcgtca gcaacaaaat atacaaaggg taggtcagca ataagatgcc
    15781 catgactacg gaatcgtagt gaccgggttc tttcaggcgg ctgatcgcat ttttcctggc
    15841 aaaaataaaa ggagggacat tgatcagcca gccgagacaa ccgggaagga acaggattat
    15901 ttttttcagg gaggatactg gaactgataa tttttctttc cgttgtttaa tgtcggtgga
    15961 aggaatttca taaacccctt tttccaattc ctgtcgcagg cggttattaa attcgaggtt
    16021 acgggggcca tcggctgtat gggctgcgat cacatcggaa gtgatgattt ccccgaagtt
    16081 tataaaaata ttttttccaa accgtgtgaa ggaactgtaa ttcaaggcaa ccggtaacac
    16141 ccgtagtgga atattttctt cccaggcttt gatcgccagc cgggcggttc cttttttcaa
    16201 gggccgcagg tgccactcgt tgatgcattt cccttcactg taaatagtga tgatgccgtt
    16261 ttgtttaaac aattccatac aggcatcaaa ggttttataa ttttcagaaa ggttttccac
    16321 gccttcactg gtccggtata cgggaagaat tttcaccgca gtcaggagtt tgcggaacaa
    16381 tggtttctta aacacatcgc cgcgggccag cgaccacaca ggctgccgga atatggtatt
    16441 gagtaaaaca gaatcgagga aagaattggg atgattggat gccagcagga ggggaccttt
    16501 ctcacgcaat ctttcagggt gattgatgac aattttccgg aaaaaaacac ggatggcaat
    16561 cccggccatt aatttcaggt agtggtagag caatggtgcg caatagtggc ctgagccggt
    16621 taaaaattaa aaatggcagc aaatccccgg aaaccaggca ctaaacggtt tgcaagggtc
    16681 gctaaatcag cgtgttacaa gagggaaact aacgaaaatt cttcgtcctg aaaagcgttt
    16741 ttccccattt ttttaaatta tctttgctgc cccgaaataa aaccattcgg gaaacaaata
    16801 tcatgtttga aaacaacatt aaaa
//
LOCUS       DAOPMW010000032        16757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3349_length_16757_cov_9.475931, whole genome shotgun sequence.
ACCESSION   DAOPMW010000032 DAOPMW010000000
VERSION     DAOPMW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16757
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_16757_cov_9.475931"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLU11_03100"
     CDS             complement(<1..403)
                     /locus_tag="PLU11_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58127.1"
                     /translation="MKFMKTAPLLLCLILLASCANEEKAPATSDSHVDAVRNFVRAAL
                     DGKFNEARMYMISDSVNNNYMDVAERSYGNMTQENKDGYRASSIQFPSPMVTLNDSIS
                     IVIYSNSFKNNPDTLKVIKTGGKWLVDFKYLY"
     gene            complement(574..1182)
                     /locus_tag="PLU11_03105"
     CDS             complement(574..1182)
                     /locus_tag="PLU11_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HPN58128.1"
                     /translation="MKKLNILLAAIALMTAIFTGCSGSAGDPKSVLVAFFDKMSKKDL
                     EGAAKLATKESKPTLDMMKKAMDMGEKFKDANTKEEDPADEFNNMEIGEAKISGDEAT
                     VSVTNKKKKETFDFPLKKQDGGWKVDFSMATLAKMGMDKAKKEGDMDQKDIDDLKNFN
                     MDSLNQGLKTLDSALKSIKPEDLEKMKNMGEEIKKQMEAAKQ"
     gene            complement(1233..1805)
                     /locus_tag="PLU11_03110"
     CDS             complement(1233..1805)
                     /locus_tag="PLU11_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPN58129.1"
                     /translation="MPVFNAAYLSRSYNQIEQSKRLIQSGDIIFRNGNDEVSEAARRM
                     NRKDSSWSHCGIILIENDSILVYHALGGTYNPDMKLLREPLAVFCNPKENYGFGVYRY
                     ELDTAQFSTIRQTVHQHYRNGLRFDLFFNFETDDVMYCSEFVFKTMNKSLHGKLSPYL
                     RIDTIPYGVTTDDLFLNPDCKPVTRENFKP"
     gene            complement(1910..2881)
                     /locus_tag="PLU11_03115"
     CDS             complement(1910..2881)
                     /locus_tag="PLU11_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPN58130.1"
                     /translation="MTDTIINLETVNPIEFFGVNNGKLDILKKKFPLLKILSRGTQLK
                     LSGAPEQIENAREKISLIVTYLERNGHMSQNYFEQILGGDDAEVIDHFKEKNPNEVLV
                     FGPNGKSVRARTANQKKLVQEAEKNDIVFAIGPAGTGKTYTAVALAVRALKNKQVKKI
                     ILTRPAVEAGESLGFLPGNLKEKIDPYLRPLYDALDDMIPADKLGYYMTTRTIEIAPL
                     AYMRGRTLDHAFIILDEAQNATDLQIKMFLTRIGSNAKAIITGDMTQVDLPRNQRSGL
                     STAVRILQNIDGIGHIELDEEDVVRHRLVKQILRAYTKEHEKDERNH"
     gene            3479..8548
                     /locus_tag="PLU11_03120"
     CDS             3479..8548
                     /locus_tag="PLU11_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN58131.1"
                     /translation="MLDAIGDAICMFDARGEFTWQNKACMTLCGMVTPEHRPPTSLTD
                     HLTDTQLVDFRKHLYRALGGERVFFEWVCANSNKGFLMELRPLRDDKQVIGVMGTVRE
                     NGNLGIAAPLSPDTGINRVFERVTDAFLALDKDWKYTYLNSKAEELHGLPANELIGKS
                     IWEVFPDVVTEPFFHSLHKAMETQQPMRVELFYSTRNRWFEDFIYPSSEGVSVYYRDI
                     TLQKQAEQQLIRSEQQYRSLVEQAADAIIIADLRGRCIDSNTMAEKMTGYGREELAGL
                     NIFSLLVITPGEPPIRMAELLNHASILQDRKVRRKDGTFFHAELNSKMISDDRILIIG
                     RDISSRKQMEMELQESEFRYRTLFEEGADGVCFYDIVKDAYIGVNKRMTELLGYSKEE
                     FLQMKVTDIAFPEEIEQNPPRYKGLAMGVTVTNERYFRRKDGRGIFIETTTRRLTGSN
                     YVSFMRDITERKQAENTIKENELRWKLALDTSELGVWEMNFEMNSAFISAKTREQTGY
                     KNETDLASPDFWLNAINKEDQASVINKFIQTLKGVLPSFDATFRVVCRNGELKWFRFT
                     GKVTDRDAAGKAKRIIGIHEHITDRVQKESELRLKGLAIEGTLSGIGMADLEGNITYV
                     NSAIVKMWGAKSSEELIGKNLAEVFHGDVVYQSMAKLRTEGAARGEDMAKRVDGSLFP
                     VEFAANVILDDKGTPACMFGSFIDITKRRDAQIRLAESETLFREIAENSSSGIILLDK
                     NFKFKFVSDSARRITGYLDDPIIGVDPDIFTHPEDLPGLLLMLQELINEPGKVVTTQY
                     RFRYKDGDWHYIESTFSNLLHIKDVEVISINFRDIHEERNARLKLQESENKLRTFFDR
                     SMDGIIIGTEDGGVVSANQSAQQMFGMTEEEMNARGKNGLVDVSDPAWAAHLQRRKEH
                     GYAISEVYMIHKDGSRFPVLFSSSVMFDAGEKKMISLIFHDISDYRRSEGQIRQFNER
                     IQLLSKATNDAVWEWDIVNDTMWWNEAYFNMMGYDPSGPVPPLPDWIRKVHPDDRDKI
                     VSRLKRIRKNMIDSWQDDFRYQLPDGTWGTVLDRAYVIRNEEGHPVRVIGAYVDITQQ
                     KLIEEKLQFEKMLSDSLIQKMPGVFYLYTRDGKFLRWNKNFETISGYNAFEIRQMHPL
                     DFYEGEEKEKVKARISHHFHETMPGADVILTTKDKRKVPLYINSMALIYEGIPCIVGM
                     GTDISTLKRTQQELVESEEAFNRLFHESNEAILLLDGRNFIDCNDATVSLLGYSSRDE
                     FLLQPPWKISPKKQPDGELSSVKAKRLISEALEKGYNRFEWIHRKADGSDFPVEVMLT
                     PILVKGKQLFYTVWRDITDRNKAELALQESIKEISAYKYAIDQATIVSFSDPTGNITH
                     VNDNFVRLYGYSKEEIIGVNHKILNSGMHSRDFWKEFWTSIRKGKVLKADVCNKAKDG
                     SLHWADTTIVPFLDEKGQPTQFLAIRNDITEKRKLEQELKERERGEQIRITETALDAQ
                     EKERNFLGQELHDNVNQILVGTKLILAVVAEDPEKYRDVLDNSIQNIQRAIEENRKLS
                     HSLATPDMKLMSLPKQLSSLAHGMFYQQPIKVSFSRKGFTEERVDDKRKITLYRIAQE
                     QCTNIIKYARATRVDIILQMKQSTIQLIIKDNGSGMEPGKSTDGIGIRNMRGRVSIYG
                     GSLQIDTAPGKGFKLTVTIPL"
     gene            8716..11184
                     /locus_tag="PLU11_03125"
     CDS             8716..11184
                     /locus_tag="PLU11_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN58132.1"
                     /translation="MLTLKMTTKPLFLLLFLLTTTIAGAQLYKVSGVVMDNKKEPLQL
                     ASVEVKELRKGTVTKDDGSFSFFLERGKYDLIVSMVGFKTRIVTFYINNEDVVENVMM
                     EIDEGPSLGEVILKVKTRDRAEELVRNVIKNKEPILNAIGSYSVNVYIRAFQMDSGVV
                     KRKKPEETDSVQRENFDGMSMTEVSLHLDKSAGGQIREERVGVNKRGGREESLFYLTT
                     TDGDFYIFNNLIQAPPVSKIPFVSPLSYSGLVAYKFKTIKIDRTVKPKIYTIAIRPRQ
                     LSNATIEGEIRIQDSTWTVFSSEFRIPSAHMPEFDYMEVKQDYGNVGDSARMITRQQF
                     NYYTKVKGGRLYGETIAVYSGYDLKKNFKRGHFGNEVSTTTQQAYEKDSLFWRTVRTE
                     PMSVQQAKYIRYQDSVYQYMRSESYLDSIDRILNKITWTKMLIFGQTFNDHKKERMWI
                     LPPITSLIQPIAFGGARLKVAGAYRKTYPSRKNLSLEADLNYGFRNRDLNGTIGLRRK
                     YNPFNQGQFNFTAGRNFEFIYPGDAWVNVLKRSNIYLNTSFEASHELEIINGVRLMNR
                     FEVAFRRSVSDYKVGNNADSIFGIPNEPPVNFDPYNATYNEIKLYLTPGLKYIREPKE
                     KIFLGSKWPTFYIIWKKGIPGLIKSEINFDYLELGLEQSINFGVMGNTSYIIKTGDFF
                     NTKDLRIIDYKFMRQGDPLFFQNPQKAFQALDSTFPIFDRYFQGNLLHEFNGALINKI
                     PFLKKLKLQEIAGGGFLFTSERALRYFEFFAGVERVFKWPFNPLARVKLGLYVVGSAA
                     NKFNNPVQVKIGLTSWDRFRNKWR"
     gene            complement(11319..11711)
                     /locus_tag="PLU11_03130"
     CDS             complement(11319..11711)
                     /locus_tag="PLU11_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HPN58133.1"
                     /translation="MKAYIAIPYQGRKEIREELDTITSILNQCGVSSFVFADQYSFKA
                     EEEKQMMQQAMEAINQSDWLIAFASEKAIGIGIEAGYAKGKGKPVIYIRHIEAEHSTT
                     LSGISDYQIIYVDLQDLAQKLIDIVEHL"
     gene            complement(11719..14130)
                     /locus_tag="PLU11_03135"
     CDS             complement(11719..14130)
                     /locus_tag="PLU11_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58134.1"
                     /translation="MRLYLRFLFLFILPASVSAQSFSFPDWSLQLSNNGAITELMDNH
                     TGRNHVVPGQPTPLVSIETKDSLLLPVSARYANHKAFLSYEGGIVITINISQRKNHLT
                     LEVTDVKNEDRIDALIWGPYPNRLGQSVGETIGIAQNDTFTLGLQALNLKTLGGFPWN
                     DNDHLPQLDALSQPGYENFDTPKKFVLYSVEAARPTWFGSSLQAYTRNRNKDRVIANW
                     GYETYTSPAYKDGGIKGSKIALFGCPTAETLNRIEEIELSEGLPHPTINGQWIKKSPV
                     INSSYLIMDFTESNMDSCIDVVKKSGLHYLYHGHPFSDWGHFNLIPEQFPLGWAGMKK
                     CVQQAAQAGIYTGTHVLSNFITTNDAYITPVPDKRLAKAGSTKILQAISATDTSIEIA
                     DPLFFNQHKNDNLHTVVIGNELIRYSTVTDKAPWKLKDCVRGAFSTTAAAHPKNEMIS
                     KLADHGYKVFLGNAELNQEIARKIASFMNYTGVRMLDFDGLEGNHSSGYGNYGEAQFA
                     QAWLDSLQPAIKNHFLLGASRSGHYFWHIYSRMNWGEPWYAGFRESQTEYRLFNQKYY
                     KRNLMPGMLGWFKMTASTTVEDIEWLMARSASYNAGFAFVTDLNTVRQNGASEEIFSI
                     LNTWENARLEGKFSPEWMERMKPLENEFKLKKTATGELWITPVYSNKFTHTKKVLQPG
                     QPAYSTYFIENKAGDQPLRFIITALDESVSGIRIELNGAKTLEIPVILEKGQTLKYTE
                     GSEALMYDNHWQIVRKIPVKASLLQCTAGKHQVQVDARFEGTNETPKLKLEFLFFGPA
                     EKLEK"
     gene            complement(14269..14529)
                     /locus_tag="PLU11_03140"
     CDS             complement(14269..14529)
                     /locus_tag="PLU11_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58135.1"
                     /translation="MNKIVVLFFLLCIGLILLIITMQTSHSKDGEFSGGLLILYGGCV
                     ILYYNRKKEKQEMAGLRFRGLVTGILCVVAGLYLILNFFFPQ"
     gene            complement(14607..15257)
                     /locus_tag="PLU11_03145"
     CDS             complement(14607..15257)
                     /locus_tag="PLU11_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58136.1"
                     /translation="MSHPHQYLKLLVVMMTSTFITQQAFGQLFLFNSNKRTDTSIWLI
                     RNKSKGIIPGNSTYFAAAVNFARSREAEFSIGRSNIIRSDYSRGIVQSNISTWGFSCA
                     FSKWAGQDQYTGKFFLEHIKIPPFLLGNFVLRTDYSVNFTNKQQYLRPSLGVTLFYAD
                     LLYSYSFKTAGPGINQYRHGFTLRSKFYASRKKAEWNYHIWKKEVWARYLRMTYKL"
     gene            complement(15254..15703)
                     /locus_tag="PLU11_03150"
     CDS             complement(15254..15703)
                     /locus_tag="PLU11_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58137.1"
                     /translation="MKIALSLCLLLTSLSLSAQFVAKMEIKEPIKGLCNEKDVYAILP
                     IGDQKEAICPVSEEEIARRLDSTVVFLKEHQDFKGKGMVSVIINCKGEVVKCEMDNKT
                     KYPELDKQIVAVFAVLGQWKAGKIGKKEVDSVKLFSFEIKNGKISLN"
     gene            complement(15810..>16757)
                     /locus_tag="PLU11_03155"
     CDS             complement(15810..>16757)
                     /locus_tag="PLU11_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58138.1"
                     /translation="LYREKKITIIGWEFGAAEYQLEADSVVTAYEFDRKKAIAIMRKS
                     MYAWCYEEYLAIFNTIWQLNKSIPEGLEKIRFLQLNKPYNPRRLYSSDPGIRQEEAKR
                     NFDNTLPGIVEKEVIQPNKKILIYCGLHHSLTKFQTPKFLFIKDKGRAGQQLFEKYPE
                     KVYQCCLLSPFPPRWFVYNELTRKENYSYVFPFEGVFNQLYDTLKRPFAVNSSNPLFA
                     ELKDHNSFYAFDTWRGIKLRNFSDGFIMLESFDKTEPVSLIRDWVTSQEETEEVKKIL
                     PETVSKDIKSPAELMQYISPENNTRELQALHQIKKFWEE"
BASE COUNT         4492 a         3648 c         3992 g         4625 t
ORIGIN      
        1 ggtacaggta tttgaaatcc accagccatt tgcctccggt tttgatcact ttgagcgtat
       61 ccggattatt cttaaatgaa ttggaataga tcacgatact gatggagtca ttcagcgtga
      121 ccatcggtga aggaaactga atggaggagg cccggtaccc gtctttgttt tcctgggtca
      181 tattgccgta ggaacgctcc gccacatcca tataattgtt attgactgaa tcactgatca
      241 tatacatacg ggcttcattg aatttaccat cgagggcggc ccttacaaaa ttgcgtaccg
      301 cgtctacatg gctgtctgaa gtggccggag ctttttcttc atttgcacag gaggccagca
      361 gaatcaggca gagcaggagg ggggcggttt tcatgaattt cataattgca aagtacaaaa
      421 gattcttaag gaaataccgg cgactggctg caatatgcct gctccgtctt tgaattcaac
      481 cgggagggag cttttttggt gtgtattcgt gttttttagt ggcaaaaaaa agccgtcaca
      541 aagtgacggc cttacgtatg taggacgggc catttattgc ttagcggctt ccatttgttt
      601 tttaatttct tctcccatat tcttcatttt ctctaaatcc tctggtttaa tggatttaag
      661 cgcactatcc agtgttttaa gcccctggtt caatgaatcc atattgaaat ttttcaaatc
      721 atcaatatcc ttttgatcca tatcgccttc ttttttcgct ttatccattc ccattttagc
      781 aagggtggcc atactgaaat caactttcca gccgccatct tgttttttca gggggaaatc
      841 aaaggtttcc tttttctttt tattggtaac agaaaccgtg gcttcgtcgc cgcttatttt
      901 agcctcgccg atttccatat tattaaattc atcagcagga tcttcttctt tggtattggc
      961 atctttaaat ttctcaccca tatccatcgc ttttttcatc atatccagcg tgggtttgct
     1021 ctcttttgtg gccagttttg cagccccttc gagatctttt ttagacattt tatcaaaaaa
     1081 ggccactaat acggatttgg ggtccccggc agatccggag catccggtga agatggcggt
     1141 cattaacgca atggctgcca ggaggatgtt caattttttc atttgttaaa tggtttaagg
     1201 agtcaaaagt aacgattacg tttaaaaaag catcatggtt tgaaattttc ccgggttacc
     1261 ggtttacaat ccgggttaag gaataaatca tcggtggtaa ccccgtatgg aatagtgtcg
     1321 atacgcaggt aaggactcaa ttttccgtgg agggatttat tcatcgtttt aaatacaaac
     1381 tccgaacaat acatcacatc atccgtttcg aagttgaaaa acagatcgaa ccggaggcca
     1441 ttccggtaat gctggtgaac ggtttgccgt atcgtcgaaa actgggcagt atccagttca
     1501 taccggtaaa ccccaaaacc ataattttcc ttcggattgc agaaaacggc aaggggttcc
     1561 cgcaataatt tcatatccgg gttataagtg ccacctaatg catggtaaac caggatgctg
     1621 tcgttttcga taagtataat tccgcaatgg gaccatgaac tgtctttccg gttcatccgg
     1681 cgggccgctt cgctcacttc atcattcccg ttacggaata tgatatcacc gctttggatc
     1741 agtctttttg attgttcaat ctggttataa ctccgggaaa ggtacgcggc attgaatacc
     1801 ggcatttgct tttttacttt gggggtgcac ccgggtaaca gccataaaag taaaaggcag
     1861 ccaattgacc gccttttaaa atattggtgt aataaataaa cgtctttcat cagtgatttc
     1921 gttcgtcttt ttcgtgctct ttcgtgtatg cccggaggat ttgcttcacg aggcggtgac
     1981 gtaccacatc ctcttcatcc agttcgatat ggccgattcc gtcaatattc tgcaggatac
     2041 gcacagcggt ggataaacca ctgcgctgat tgcgcggcag atccacctgg gtcatatccc
     2101 cggtaataat ggctttggca ttggaaccaa tacgggtgag aaacattttt atttgaaggt
     2161 ccgtggcatt ctgggcctca tccaggataa taaaggcatg atcaagtgtc cgtccgcgca
     2221 tatacgccag gggggcgatc tcgatggtgc gtgtggtcat ataatacccc agtttatccg
     2281 ccgggatcat atcatccagg gcgtcataga gtggacgcag gtagggatcg atcttctcct
     2341 tcaggttgcc gggcaggaaa ccaaggcttt cgcccgcttc taccgccggg cgggtaagga
     2401 tgatcttctt gacctgttta ttcttaagcg cccttaccgc cagggcaacc gccgtatagg
     2461 tcttaccggt accggcgggt ccgatggcaa atacgatatc gtttttttcc gcttcctgta
     2521 ccagtttttt ctgattagcc gtgcgggccc ttactgattt gccattgggt ccaaagacca
     2581 gcacttcatt gggatttttc tccttaaaat ggtcaatgac ttccgcatca tccccaccca
     2641 gaatctgctc aaaatagttc tggctcatgt gcccgttacg ttccaggtag gtgacgataa
     2701 gggaaatttt ttcccgggca ttttcaatct gttccggggc cccgctcagc ttcagttggg
     2761 taccgcgcga gaggattttc aacaggggaa attttttctt aaggatgtcg agctttccgt
     2821 tgtttacacc aaaaaattca atagggttta cggtttcaag gttaatgata gtgtctgtca
     2881 attgcttcgg tttatgtttc tcaaagccgg caaaagggcg gtaccggatt atctgcgtct
     2941 tctgcgcttt tacagaagcc tgcccttctt ttccaatatt aatcagcaat actgtaaata
     3001 cctgcactta cttattcaca ggttgtgaaa agagaaaagg gggctagtga tggacggatt
     3061 gcttttttct gtatagcagt ctgactttca ggccggccag gaaataccgg ccggtaaagc
     3121 attccggttt tttgcagggc actgattttt ttacataacg gatatgtata gttgtaaacc
     3181 ggattttccg ctgctgcttg ctgcaagagc aaacttgtcc attggggtca aattcatata
     3241 aagcatttat ttactatatt ctgccagata agggtaaact attataaaaa aataaatatt
     3301 atacgaatgc cggctgaata gtattgctta aatgacgggt tttcaagggt attgactaca
     3361 ttatgtgaga accgcttttt gtattgccaa cactatgcct actggatcac accgccatcc
     3421 accagatcgt ttaaccgagg tagctacggc ttcaccggat cgctatttgt acccgcttat
     3481 gcttgatgcc ataggcgatg ctatctgcat gtttgatgcc cggggagaat tcacctggca
     3541 aaacaaggcc tgcatgacgc tctgtggaat ggttacgccg gagcatcggc cgccaacctc
     3601 cctgacggat catttaacgg atacacaact ggtcgatttc cggaaacatt tgtaccgggc
     3661 gctgggggga gaacgggtat tttttgaatg ggtctgcgcc aatagcaata agggattcct
     3721 gatggaactc cggcccttac gtgatgataa acaagtgatt ggggtgatgg ggacggtccg
     3781 tgaaaacggt aacctgggca ttgctgcgcc gctgtcgcct gacacgggta taaaccgtgt
     3841 ttttgaacgg gtaaccgatg ccttcctggc cctggataag gactggaagt atacttatct
     3901 caatagtaaa gcagaagaac ttcatggttt accggccaat gaactgatcg ggaaaagcat
     3961 atgggaagtg tttccggatg tggtaacaga acccttcttc cacagcttgc ataaagctat
     4021 ggaaacccag caacccatgc gggttgagct tttttattcc acccgtaaca gatggtttga
     4081 agacttcatt tatccatcat cggaaggagt ctctgtttat taccgggata tcactttaca
     4141 gaaacaagcg gaacagcaat tgatccgcag tgaacaacaa taccgctccc tggtggagca
     4201 ggcggctgat gcgatcatca ttgccgatct gcgtggccgc tgtattgaca gcaataccat
     4261 ggcggagaaa atgaccgggt atgggcggga ggaactggcc ggactcaata ttttcagttt
     4321 attggtaatc acgccgggag aacctcctat acgtatggcg gagttactca atcatgcatc
     4381 tattttgcag gatcggaaag tgcggcggaa ggacggaacc ttttttcatg cggaactgaa
     4441 ttcgaaaatg atcagtgatg accggatcct gatcattggc cgggatatca gctcgcgtaa
     4501 gcaaatggaa atggaactcc aggagagcga gttcaggtac cggactcttt ttgaagaagg
     4561 ggcggacggg gtttgctttt atgatattgt aaaggatgcc tatatcgggg taaataaacg
     4621 aatgaccgaa ttattaggat actccaaaga agagttttta cagatgaaag taacggacat
     4681 cgcgtttccg gaggaaatag aacagaatcc tccccgatac aagggactgg ctatgggagt
     4741 tacggtaacc aatgagcgct atttcagacg caaagatggc cgtggaatat ttattgaaac
     4801 caccacccgg cggctgaccg ggagtaatta tgtttcgttt atgcgggaca ttaccgaaag
     4861 gaaacaggca gaaaacacga tcaaagaaaa tgaattacgc tggaagctgg ccctggatac
     4921 cagcgagctc ggggtttggg aaatgaactt tgaaatgaac agcgccttta tatcagccaa
     4981 aacccgtgaa cagacaggct ataaaaatga aacggatctg gccagtcccg atttctggtt
     5041 aaatgccatc aataaagagg atcaggcttc cgtgattaat aaatttattc aaacactcaa
     5101 aggggtcctg ccctcatttg atgccacatt cagggtggtt tgccggaatg gagaattgaa
     5161 gtggttccgg tttaccggta aagtgacgga tcgcgatgct gccggaaaag caaaacgcat
     5221 tatcgggatt catgaacata taacagaccg ggtacagaag gaaagtgaac tccggctcaa
     5281 aggactggcc attgaaggta cactcagcgg aattggcatg gcagaccttg aagggaatat
     5341 tacctatgta aattcggcaa tcgttaaaat gtggggggcg aaaagcagtg aagaactgat
     5401 tggaaaaaac ctggctgaag tcttccatgg ggatgttgta tatcagtcca tggcaaaact
     5461 tcgtacagaa ggggctgccc gtggtgagga tatggccaag cgggtggatg gatcgctttt
     5521 ccctgttgaa tttgccgcca atgtaattct cgacgataag ggaacgcccg cctgtatgtt
     5581 cgggtctttt attgacatta ctaaacgccg ggatgcacag atccggttgg cggaaagcga
     5641 gacattgttc cgggaaatcg ctgaaaattc ctcaagcgga atcatactac tggataaaaa
     5701 cttcaaattc aaatttgtca gtgattccgc ccgccggatc accgggtacc tggatgatcc
     5761 aataatagga gtggatccgg atatcttcac ccatccggaa gatttgcccg ggttgctgct
     5821 gatgctgcag gaactgatca acgaacccgg aaaagtagtc actactcaat accggttcag
     5881 gtataaggac ggagattggc attatattga aagtactttc tccaacctgc ttcacattaa
     5941 agatgtggaa gtgatatcca tcaatttcag ggatattcat gaagaacgaa atgcccggtt
     6001 gaaattgcag gaaagtgaga ataaactccg gactttcttt gaccgtagta tggacgggat
     6061 catcattggc acagaggatg gcggggtggt gtcagccaat cagtcggccc agcagatgtt
     6121 tggaatgacc gaggaagaaa tgaatgcacg tgggaaaaac ggcctggtag atgtgtctga
     6181 tcccgcctgg gcggcgcatc tgcaaagacg aaaagaacat ggttatgcca tcagcgaagt
     6241 ctatatgatc cataaagacg gatccaggtt ccctgtcttg ttttcttcct cggtaatgtt
     6301 tgatgccggg gagaaaaaaa tgatcagcct gatttttcat gatatcagtg attaccggag
     6361 aagtgagggg cagatccggc aattcaatga acgaatacag ctactatcga aggctaccaa
     6421 tgacgcggta tgggaatggg atattgtaaa tgatacgatg tggtggaatg aggcctattt
     6481 caacatgatg ggttatgatc cgtccggacc ggtaccaccg ctcccggact ggatccggaa
     6541 agtgcatccg gacgaccgcg acaaaatcgt cagccggtta aaacggatcc gtaaaaacat
     6601 gatcgactcc tggcaggacg atttccggta tcagttgccc gatggtacct ggggtaccgt
     6661 actcgacagg gcctatgtga tccgcaatga agaaggacat ccggtccggg taatcggggc
     6721 ttatgtggat attacccagc aaaaactgat cgaagagaaa ttgcagtttg aaaaaatgct
     6781 ctcggattca ctgatccaga aaatgccggg cgtattttat ctctatacgc gggatggtaa
     6841 attcctgcgc tggaataaaa attttgaaac gatctccggg tacaatgctt ttgagatccg
     6901 ccagatgcac cctcttgatt tttacgaagg agaggaaaag gaaaaagtaa aagccaggat
     6961 cagtcatcac ttccatgaaa caatgcccgg tgcggatgtg atcctgacca ccaaggataa
     7021 acggaaagtc ccgctttaca tcaattccat ggcgttgatc tatgaaggaa ttccctgtat
     7081 cgtaggaatg ggtactgata tctccaccct caaacgtacc cagcaggaac tggtggaaag
     7141 tgaagaagcc tttaaccggc tgttccatga atccaatgaa gccattcttt tgctggatgg
     7201 ccggaatttc atagactgta atgatgctac ggtctcttta ttaggctatt cgtcaaggga
     7261 tgaattcctg ttacaacctc cctggaagat ttcacctaag aaacaacccg atggagaatt
     7321 gtcgtctgtt aaagcaaagc gactgatcag cgaagccctg gaaaaaggat ataaccggtt
     7381 cgaatggata caccggaaag ccgatggatc tgattttccc gtggaagtca tgctgacccc
     7441 gatactggtc aaagggaagc agttgtttta taccgtctgg agagatatta ccgaccgcaa
     7501 taaagcggaa ctggcactgc aggaaagcat aaaggaaatt tcagcgtata aatatgccat
     7561 tgatcaggca actattgttt cattttcaga tccaaccggt aatattacgc atgtgaatga
     7621 taattttgta cgattatacg gatattccaa agaagaaatc atcggcgtga atcacaaaat
     7681 cctgaattcg ggtatgcatt cccgtgattt ttggaaagaa ttctggacct cgatcaggaa
     7741 aggtaaagtg ctgaaagcgg atgtttgcaa taaagccaag gacggctccc tgcactgggc
     7801 cgataccacc atcgttccct tcctcgatga aaaggggcag cccactcagt ttctggcgat
     7861 ccggaatgat ataactgaaa agcgaaaact ggaacaggaa cttaaagaac gggaaagagg
     7921 cgaacagatc cgcataacgg aaaccgccct ggatgcgcag gaaaaggaac ggaacttcct
     7981 gggacaggag ctgcatgata atgtaaatca gattctcgtc ggaacaaagc tgatcctggc
     8041 cgtggtggca gaagatccgg aaaaataccg ggatgtgctt gacaacagta ttcagaacat
     8101 acaaagggcc attgaagaaa accggaagtt gtcacattcc ctggcaactc cggatatgaa
     8161 actgatgagc ctgcctaaac aactgtccag cctggctcat ggtatgtttt accagcaacc
     8221 tattaaagtg tctttcagcc gtaaaggatt cacggaagaa cgggttgatg ataaacggaa
     8281 aatcaccctg taccggattg cccaggaaca atgcacgaat atcattaaat atgcccgggc
     8341 cacccgggtg gacatcatcc tgcaaatgaa acaatccacg attcagctta tcattaagga
     8401 taacgggtca ggaatggagc ccggaaagag taccgatggt atcggcatcc ggaatatgcg
     8461 cggtcgtgtc agcatttacg gaggctcatt acaaattgat acggctccgg gaaaggggtt
     8521 taaactcaca gtaactattc cactctgaaa ggatcaccta ccggttatcg gacaataagc
     8581 aaaataatca gctatttttg caaggcaata aggtatagac ccctgcccgg tgcccgggat
     8641 gttaaaaaaa gctaaaatgc atccatgcgt caggatatgc cgttacaaat tgcaacattt
     8701 gtagtttaac ctgtaatgct taccctgaag atgaccacta aacccctttt tctcctcctc
     8761 tttctcctga ctactactat agcaggcgca cagttgtata aagtatccgg ggtggtgatg
     8821 gataataaaa aggaacccct gcagctggcc agcgtggaag taaaggaact ccgtaaagga
     8881 acggttacca aagatgatgg ctctttcagt tttttcctgg aaaggggtaa atatgacctc
     8941 atagtgagca tggtgggatt taaaacaaga atcgtcactt tttatatcaa taatgaagat
     9001 gtagtggaaa atgtgatgat ggaaattgat gagggcccca gcctggggga agtgatactc
     9061 aaagtcaaaa cccgggaccg ggcagaagaa ttggtccgga atgtgatcaa aaacaaagaa
     9121 ccgattctga atgcaatcgg ctcctattcc gtgaatgtct atatccgcgc cttccagatg
     9181 gattcagggg tggtgaaaag aaaaaagccc gaagaaaccg atagtgtaca acgggagaat
     9241 tttgacggaa tgtccatgac tgaagtgtcc ctgcatctcg acaagagtgc cggcggacaa
     9301 atccgggagg aacgcgttgg cgtgaataag cggggagggc gggaagaaag cctcttttac
     9361 ctgaccacaa cagacggcga cttttatata ttcaacaacc tgatacaggc accgccggtg
     9421 tcaaaaattc ccttcgtatc gcctttgagt tacagtggac ttgtggcgta taagttcaag
     9481 acaatcaaaa tcgaccggac tgttaaaccc aagatttaca cgattgccat tcggccgcgc
     9541 cagttgagta atgcaaccat cgaaggggag ataaggatac aggacagtac atggaccgtt
     9601 tttagttccg aattcaggat accctctgcc catatgccgg agtttgacta tatggaagta
     9661 aaacaggatt atggtaatgt aggtgacagt gcccggatga ttacccggca gcagtttaac
     9721 tattatacca aagtgaaggg cggacgcctt tatggcgaaa ccatcgctgt ctattcgggt
     9781 tacgacctga aaaagaattt caaaagagga catttcggca atgaagtcag taccaccacc
     9841 cagcaggcgt atgaaaaaga ttccttgttc tggcgtaccg tacgaactga gccgatgtcg
     9901 gtccagcagg ccaaatatat ccgttatcag gacagtgtgt atcagtatat gcggagtgaa
     9961 tcttatctcg attccattga ccggatactg aataaaatca cttggaccaa gatgctgatc
    10021 ttcggacaaa ctttcaatga tcataagaag gaacggatgt ggatactgcc tcccattact
    10081 tccctgatcc agccgattgc ctttgggggt gcccggttaa aagtagcggg cgcctaccgc
    10141 aaaacctatc cctctcgtaa gaacctctcc ctggaagccg acctgaacta tggattccga
    10201 aaccgggacc tgaatggcac catcgggtta agaagaaaat ataatccgtt taaccaggga
    10261 cagttcaatt tcaccgccgg acggaatttc gaattcatct atcccggtga tgcctgggtg
    10321 aatgtgctga agcggagtaa tatttatctg aatacgtcct ttgaagccag tcatgaactg
    10381 gaaatcatca acggggtccg gttgatgaat cgttttgaag tggctttccg ccgttccgtc
    10441 tccgattata aagtgggcaa taacgccgat agtatttttg gtattcccaa tgaaccaccg
    10501 gttaacttcg atccgtataa tgccacgtat aatgagatca agctttatct caccccggga
    10561 ctgaaatata tccgcgaacc gaaagaaaag atttttcttg gttctaaatg gccgacattc
    10621 tatattatct ggaaaaaagg aatccccgga cttattaaaa gtgaaatcaa ttttgactac
    10681 ctcgaactgg gactggagca gagtatcaac tttggggtga tggggaatac ctcttatatc
    10741 attaagaccg gtgacttttt taacaccaaa gacctgcgta ttatcgatta taaatttatg
    10801 cgccagggcg atccgttgtt tttccagaat cctcaaaagg ccttccaggc actggactcc
    10861 actttcccga ttttcgaccg ttatttccag ggcaacttgc tccatgaatt caacggggcc
    10921 ctcataaata aaattccctt cctgaaaaaa ctcaaactcc aggagatcgc aggagggggc
    10981 ttcctcttca ccagcgagag agccctgcgt tatttcgaat tctttgccgg ggtggaacgg
    11041 gtattcaaat ggccattcaa ccccctggcc cgcgtcaaac ttggtttgta tgtggtgggt
    11101 tctgccgcga ataaatttaa taatcctgtg caggtgaaga tcggattgac gagttgggat
    11161 cggttcagga ataaatggcg ttgaggcagt aggcagtagg cagtcggcag aatgtagttg
    11221 tcatcctgag cctgcctacc ggacaggcag gcctgccgaa ggatgaagta gtaggcagtt
    11281 agtttttcag aagaaagcat ggggtttggt tgctataatt ataagtgctc aactatatct
    11341 attagttttt gtgctaaatc ctgcaaatcg acgtagataa tttgataatc actgatgccg
    11401 gagagggtgg tggaatgttc agcttcaatg tgcctgatat aaattacggg tttacctttt
    11461 cctttggcat agccggcttc gataccaata ccgatggctt tttcggaagc aaacgcaatc
    11521 agccaatctg attgattgat ggcttccatg gcctgctgca tcatttgttt ttcttcttcc
    11581 gctttgaatg agtactgatc tgcaaatacg aatgaactga ctccacattg attcaaaata
    11641 ctggttatag tatccagttc ttcccgtatc tcttttctgc cctggtaagg aatggcgata
    11701 tacgctttca tggctaattt atttttccag tttctctgcc ggaccaaaga aaaggaattc
    11761 gagttttagt ttgggcgttt cattcgttcc ctcaaaccgg gcatccactt gcacctgatg
    11821 tttgccggca gtgcattgaa gcaaggatgc cttaacggga atcttccgga ctatttgcca
    11881 gtgattatca tacatgaggg cttcggaacc ctccgtatat ttcagggttt gtcctttttc
    11941 taaaatgacg ggaatctcca gtgtcttagc gccattcagt tcaatccgga ttccggaaac
    12001 ggattcatcc agggcggtga taataaagcg aaggggttgg tccccggctt tgttttcaat
    12061 gaagtatgta ctataagctg gttgaccggg ttgaagtact ttttttgtat gcgtaaattt
    12121 attggagtag accggtgtaa tccataattc gccggttgct gtttttttga gtttgaattc
    12181 attttccaga ggcttcatcc gttccatcca ctccggggaa aactttccct ccagccgggc
    12241 attttcccag gtgttgagga tggagaagat ctcctcactg gctccgtttt gtcttaccgt
    12301 attcagatcc gttacaaagg caaaaccggc gttataggaa gcggacctcg ccatcagcca
    12361 ttcaatatct tctaccgtgg tggaagccgt catcttaaac cagcccagca taccgggcat
    12421 caggttgcgt ttataatatt tctggttgaa taaacgatac tccgtctggc tttcccggaa
    12481 acccgcatac cagggctcac cccagttcat ccgcgaatag atatgccaga aataatgacc
    12541 ggaacgactg gctccgagta agaaatgatt tttgatagcg ggctgcagac tatccagcca
    12601 ggcctgggca aactgagctt ctccatagtt gccataaccg gaagagtgat tgccttccag
    12661 tccgtcgaag tccagcattc ttacacctgt ataattcata aaggaagcga tcttccgggc
    12721 gatctcctgg ttcagttccg cattccccag gaatacttta tagccatgat cggccagctt
    12781 agagatcatt tcgttttttg gatgtgcagc ggccgttgta ctgaaggcac ctcttacaca
    12841 gtctttcagt ttccagggtg ctttatctgt aaccgtgctg taccgtatca gttcattacc
    12901 gatgaccacc gtgtgcagat tatcgttttt atgctggttg aagaataatg ggtccgcgat
    12961 ctcaatactg gtatccgtag cacttattgc ctgcaggatc tttgtactac cggcctttgc
    13021 caaccgttta tccggaacgg gtgttatata ggcatcattg gttgttatga aattggagag
    13081 tacatgagtg ccggtataaa tgcctgcttg cgctgcctgc tgtacacatt ttttcatgcc
    13141 ggcccatccg aggggaaatt gttccgggat taaattaaaa tgtccccagt cgctgaaagg
    13201 atgaccatga tagagataat gcaatccgga tttcttcacc acatcaatac aactgtccat
    13261 attgctttcc gtaaaatcca tgatcaggta tgaagaattg atcacaggtg acttctttat
    13321 ccattggcca ttgatcgtgg gatgagggag tccttcactg agttcaattt cttcaattcg
    13381 attcagtgtt tcggccgtgg gacagccaaa caaggcaatt ttagagccct tgatacctcc
    13441 atctttatag gcaggggagg tgtaagtttc ataaccccag ttcgcgatca cccggtcttt
    13501 attccggtta cgggtataag cctgcagcga acttccaaac caggtaggac gggccgcttc
    13561 tacggagtat aggacaaatt tcttcggggt atcaaaattt tcatagccgg gttgggaaag
    13621 ggcatccagc tgtggcaggt gatcattatc gttccaggga aagccgccca gggttttgag
    13681 gtttaatgcc tgcaatccca aggtgaatgt atcattctgt gcgattccga tggtttctcc
    13741 taccgattga ccaagacggt tggggtaagg tccccagatc agggcatcga tccggtcttc
    13801 gttttttaca tctgttactt caagggtcag gtggttttta cgttgggata tgtttattgt
    13861 tatgacgatg cccccctcat aggaaagaaa tgctttgtgg ttggcatacc tggcgctgac
    13921 tggcaataaa aggctgtctt ttgtttctat ggaaacgagt ggagtgggtt gtccgggtac
    13981 cacatgattc cggcctgtat gattatccat caattcggtg atcgctccgt tgttgctcag
    14041 ctgcagtgac cagtccggga aagaaaaaga ttgagctgaa accgaagctg gcaaaataaa
    14101 cagaaaaagg aatcgcagat acagtcgcat ggcagttgtt ttgtgatagg cgggtcaaag
    14161 caatcaccat atatttgtac aatttgtgct tgctttcact tctaaattag ggatttttta
    14221 tcaggtccgg tgttgcgtaa gcctgaatca aatcataaga agaaaaggtt attggggaaa
    14281 gaaaaaattc aggatcaggt ataatccggc caccacacat aggattccgg tcaccagtcc
    14341 cctgaaccgg agcccggcca tttcttgctt ctcttttttc cggttataat aaagtataac
    14401 acaaccacca tacaatataa gcaggccacc actgaactcg ccatctttcg agtggctggt
    14461 ctgcatcgta attatcagga gaatgagccc gatacaaagc agaaagaata gtacaacaat
    14521 tttattcatg tttgcgttgt ttgtgtcaaa ccggcctggc gggtagtgaa agacaaaaat
    14581 ggctcagtgt ttttcattca gaaatattaa agcttatagg tcattcttaa atacctggcc
    14641 catacttctt ttttccagat atgataattc cattcggcct ttttccggct ggcatagaac
    14701 tttgacctta atgtaaaacc atgccggtat tgattgatac ctggcccggc cgttttgaat
    14761 gaataactgt agagcagatc tgcgtagaac aaagtgacac ccagtgaagg gcggaggtac
    14821 tgttgtttat tggtaaagtt gaccgaataa tcggtgcgca gcacaaaatt acccaacaag
    14881 aacggtggga ttttgatatg ttcgaggaaa aattttccgg tatactggtc ctggcctgcc
    14941 catttcgaaa atgcacagct gaaaccccat gtgcttatat tggattgaac gatccctctc
    15001 gagtaatcgc tcctgatgat attcgaccgg ccaatactga attcggcttc ccggctacgg
    15061 gcaaaattga ccgctgcggc aaagtaagtg ctgttgccgg gtatgatccc ttttgattta
    15121 ttccggatca gccatatcga agtgtcggtt cgtttattgg aattgaaaag gaacagctgt
    15181 ccgaatgcct gttgcgtgat gaaagtactg gtcatcataa caaccagcag tttcaggtat
    15241 tgatgcggat ggctcaattc aggctgattt ttccgttttt aatctcgaaa ctgaacaact
    15301 tcacagagtc tacttccttc tttccgatct ttccggcttt ccattgaccc agaacagcaa
    15361 aaacagcaac tatttgttta tccaactcag gatattttgt cttattatcc atttcacatt
    15421 tcactacttc tcccttacag ttaataatta cactgaccat ccctttccct ttaaaatcct
    15481 ggtgttcttt caggaaaaca accgttgagt caagccgcct ggcaatttcc tcttcactta
    15541 ccgggcatat ggcttctttc tggtctccta ttggtaaaat agcatacacg tctttttcat
    15601 tgcaaagtcc tttgatgggt tctttgattt ccatttttgc aacaaactgg gcagaaagcg
    15661 aaaggctggt taaaagcagg caaaggctaa gggcaatttt catttgatag gttttttgtg
    15721 tataggataa aggtagataa tttttgattt tagcacttgt cgggctcctg atttgtacat
    15781 gttttttggc aagggtgttc tgaaaacagt tactcctccc agaatttttt gatctggtgc
    15841 agcgcctgca actccctggt attattttcc gggctgatat attgcatcag ttcagccggg
    15901 gatttaatat cttttgatac ggtctccggc agtatttttt ttacttcttc agtctcttcc
    15961 tggctggtta cccaatcccg gatcagtgaa accggttcag ttttatcaaa tgactcaagc
    16021 ataatgaagc cgtcactgaa attgcggagt ttaattcctc tccaggtgtc aaacgcataa
    16081 aaactgttat gatctttcag ctctgcgaat aaagggttac tgctatttac ggcaaaaggt
    16141 ctttttaagg tatcatagag ttgattgaat actccctcaa aagggaaaac gtaactataa
    16201 ttttctttgc gggtcagttc attatacaca aaccaccggg gtggaaatgg agaaagcagg
    16261 caacattggt aaaccttctc cggatatttt tcaaatagtt gctgaccggc ccgccctttg
    16321 tcttttataa aaaggaattt tggagtctgg aattttgtca gcgaatgatg aaggccgcaa
    16381 taaattagta ttttcttatt gggttgaatt acttctttct ccacgatgcc gggaagggtg
    16441 ttgtcaaaat ttctttttgc ttcctcctgg cggatgccag ggtctgaaga atacaaacgc
    16501 ctggggttat acggcttgtt cagttgcaga aaccggattt tttccagtcc ctctggaata
    16561 gatttattta attgccatat tgtgttgaat atggccaggt actcttcata gcaccaggcg
    16621 tacatcgatt ttcgcatgat ggctatagct tttttgcggt caaattcgta tgctgtaacc
    16681 acactgtccg cttccagctg atattcagcg gctccgaatt cccaaccgat tatagtgatt
    16741 ttcttttccc ggtatag
//
LOCUS       DAOPMW010000033        16401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3464_length_16401_cov_8.274318, whole genome shotgun sequence.
ACCESSION   DAOPMW010000033 DAOPMW010000000
VERSION     DAOPMW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_16401_cov_8.274318"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..496)
                     /gene="raiA"
                     /locus_tag="PLU11_03160"
     CDS             complement(197..496)
                     /gene="raiA"
                     /locus_tag="PLU11_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPN58139.1"
                     /translation="MNVKIQTVHFNADSKLMEYVNKKLEKLTVFSDRIIQVHVFLKLD
                     NVVHSVKDKIAEIRVHVPRHDFFVKSTSKSFEESFDSAFDSLVSQIKRKKEKQAA"
     gene            complement(653..1555)
                     /locus_tag="PLU11_03165"
     CDS             complement(653..1555)
                     /locus_tag="PLU11_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPN58140.1"
                     /translation="MPVQERANIQTFLDYLKFEKRYSAHTLLAYTNDLTDFFDFLEIQ
                     YGIRELREIQSGIVRSWLASLKDKGLGAVTLNRKISSLKSFFKYQLKTGGVEQSPMGQ
                     VISPKKSKRLPVFVKEEEVRELVQTLSGAAEDWKSLNMKMLVAIFYATGMRLSELTGL
                     KETQVDESRGQIKVLGKGNKERVIPVSPELMAAIREYQQLKRKEFEQTGETLLVTEKG
                     KKLYPRYAWALVNQALGEASTLDKKSPHVLRHSFATHLMNHGADLNAVKELLGHSSLA
                     ATQVYTHNTIGKLKDIHKKAHPRG"
     gene            complement(1626..1820)
                     /gene="rpsU"
                     /locus_tag="PLU11_03170"
     CDS             complement(1626..1820)
                     /gene="rpsU"
                     /locus_tag="PLU11_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPN58141.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKSKVLLQLRERQSFIKPSVR
                     RRGQVLKAIYKQQLATGKFD"
     gene            1921..2511
                     /locus_tag="PLU11_03175"
     CDS             1921..2511
                     /locus_tag="PLU11_03175"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPN58142.1"
                     /translation="MFTGIIEHIGEVKEVISNGSNRSFWIESPISDQLKTDQSVSHSG
                     VCLTVEEVSGNRHRVTAIDETLNKTNLGSWGPGSLVNLERCLQMNGRLDGHLVQGHVD
                     ATGTCTGKTVKEGSWEFEIAFPRPFAELVIEKGSICLNGISLTAFEVKKKHFKVAIIP
                     YTFEHTNLKTLEPGQTVNLEFDMIGKYLLRRLSLKD"
     gene            2532..3617
                     /locus_tag="PLU11_03180"
     CDS             2532..3617
                     /locus_tag="PLU11_03180"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPN58143.1"
                     /translation="MNPAPAHRQYYPALDGLRGLAILLVIVYHNFGFINYFFFGWLGV
                     DLFFVLSGFLITDILLNTAGQPNFLKNFYTRRLLRIFPLYYLALILFIYIIPGVFQTF
                     PTGYYRENQFWMWTYLQNWLYIFKPAGETTGLHHLWSLAVEEQFYLLWPLIILLLKKP
                     GRLLLFISLVLLAVIGLRIWIWNSQVENLAYFNLYTFSRIDGLCIGSMVALLHRINPG
                     FLSKNTSVVVLCFALANFAFYFINQQYAFSFPYLAIAGYTTFAMTFGLLVNEAVTNGT
                     RLITTVFSWPVLRFFGKISYGLYVFHWPLYLILNPVLTQVISIPGNSTLTSFLGSLLS
                     TGIAIGISLLSFHYFEQPFLKLKQRFN"
     gene            3632..4396
                     /locus_tag="PLU11_03185"
     CDS             3632..4396
                     /locus_tag="PLU11_03185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58144.1"
                     /translation="MDLVEYNLIKEKSFRRHPWEQTRMQFLFYLLNRLPNKNNLADTG
                     SGDGYLASGIATRYPEARVTAIDINYTDELLEALHTGKPANLRFTRDLHVIPDDPPAD
                     AIILMDVLEHIEKPEELLKELLQLKAVNSETRFIITVPAFQKLFSMHDKNLGHYRRYN
                     LKQLEKLLSPLSLRMENSGYFFNSLLPVRYLQKLVEKNKPVKDKDSIHNWKGGPLITW
                     ILRTGFWIEFKITWYLARLGIKIPGLTCYCICRYAP"
     gene            4378..5112
                     /locus_tag="PLU11_03190"
     CDS             4378..5112
                     /locus_tag="PLU11_03190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN58145.1"
                     /translation="MPVRSIIIPCHNEALRLEPGAFSAFLQQSAGTHLLFVNDGSTDN
                     TQQVLEQIRNNNPDQVTILSLTSQSGKGEAVRRGMMESLSLFPAVTVSGYMDADLSVS
                     LDEMTRLFGCFSHAEKRFILGSRVKKIGAQISRNEWRHFYSRIIATIVGSIIRLDVYD
                     TQCSAKLFRQDSIETVFNKPFRTKWLFDVEIICRLHKAYGPLNDNGLEEPLLEWTERK
                     GSKLRWYSIFRVIREVFILKKYYTGK"
     gene            5109..7298
                     /locus_tag="PLU11_03195"
     CDS             5109..7298
                     /locus_tag="PLU11_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58146.1"
                     /translation="MTESGNNKKYQDYLFIIVLALLAYWPVSFMLFSVKNDAINYFLA
                     MRYNTSEAIQSGHFPLWSPYINLGYPLHGDMQSGTWNPVVLVMSLVRKYDIYWLQLET
                     IITIIISGCSFYKLLSHFQLSRVILLAGSAAYMLNGYITDAGQFLNWLYAAAYLPLVV
                     LYAIRSFEQFSTRDAFLLGVSFSLMLLSAYPADFILLGYLLTAYFLFSVFRYKKDSGL
                     IKSFTRCLKPALIAVVSFALICLPAILSYLPFLASIRRGNGVELDVALSNSFSPVNFI
                     SVLTPWPTQRLNWEQYTDPLIRNSYMGLIPLVFLITYFRQKSKKTFVQYFFTGTLIFF
                     LLFSLGKAGGLRVLSYYTLPLLDSFRHPANAKLFVLFAAQLLSFLTLDKYLKEPGSLQ
                     PAIKRIVNILLVITGLLLITGLFKTRIFQLAGKLNVTHGRLDLKTIFDAISFYDFLFL
                     NATLAFIILLMLRWTLKKNRLQRWLLPVLCAEMILAAQFMLPLTYFRTSSPQTVQNIL
                     NAQPKGYPAPDGFTSIREMSADGMNYFNEIGCLNPFNKKPGRSDYIITPANLSWQLDF
                     WEDTAFREKIIEYPFYYLADTIYQVPDKKDFIRDTLSRKAALANLSLYAKPLIPDNQD
                     ALSVIESFGPEHIRFKVNSAGPTFLVLFQNHYPNWAVRINGQKTDIIPTNHAFMGVVV
                     PGGEQVVEFRYEAPGILYMAMGSLLFTLAGLVYFSRKKKNTREQKAD"
     gene            7279..7986
                     /locus_tag="PLU11_03200"
     CDS             7279..7986
                     /locus_tag="PLU11_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58147.1"
                     /translation="MNRKLTNTIRFVMDEGLPAFIRDSRWFMYPFFWYWFKGKNLATY
                     MDFKKIAWGLSEAAFNRVYQELDCRATDRPTDLNEKCIRLMLEKLHPEAKTMLDVGCG
                     RGHWLSLAGGARKDLLLTGCDVFEESTVNKSFTFKQGSIYQLPFEDRSFDIVTCHHTI
                     EHLPDIHKAISELKRVTRKQLIIVTPRQRYFYYTLDLHLHFFPEKEYLQQVTGIPNGD
                     YMDCDGDWCLIANTGSI"
     gene            complement(7979..9838)
                     /gene="dnaK"
                     /locus_tag="PLU11_03205"
     CDS             complement(7979..9838)
                     /gene="dnaK"
                     /locus_tag="PLU11_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPN58148.1"
                     /translation="MAKIGINIATGSLQQHEMIVGIDLGTTNSLVAIIHPDTRQPVVL
                     KEHDGVSLVPSIVHFGETGQVTVGEEAKKFLITEPQHTIFSVKRLMGKSYNDIREHSG
                     YFSYKVIDDHTESLVKIQVGEVFYSPVELSSLILKELKARAEHILKTPVTKAVITVPA
                     YFNDAQRQATRDAGKLAGLDVLRIVNEPTAASLAYGLGLSRDEIKTIAVYDLGGGTFD
                     ISILRISNGIFEVLSTNGDTYLGGDDIDRAIVQYWASLAGITDEELKMHKNLSQELRL
                     LAETAKIALSSSDNYIGEVAEQEFSLSREQLEQLITPLIDRTITSCAGAMKDAGLATS
                     DIDEVVMVGGSTRVPLVKQRVSAFFGKKVHDTLNPDEVVALGAAVQADVLAGNNKDLL
                     LLDITPLSLGIETMGGLMDVLIPRNSKIPSKAGRQYTTQKDGQGSMRISVFQGERDLV
                     KDNRKLAEFNLTGIPGMPAGLPKVEVSFLVNADGILTVKAKELRSGVEQSIDVKPQYG
                     LSDAEVEKMLMDSITNAKEDIRTRALVEAQTEALQILETTRQFIQRNTGFLTDMELAE
                     TEKAMEELTRLREQGTKDAIQAGVEKLNDISRPYAERLMDTAVAAALKDKHIK"
     gene            complement(9982..15111)
                     /locus_tag="PLU11_03210"
     CDS             complement(9982..15111)
                     /locus_tag="PLU11_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPN58149.1"
                     /translation="MKRLLLLLLLISGLAAQSQTYNNEWIDYSKTYYKFKVGVNGLYR
                     ISQPVLASLGIGNTPAQDFQLWRKGEQVPMYTTVQTGTLGAGDYLEFWGEMNDGKSDN
                     ELYRNADYQLNDKWSLETDTVSYFLTINAASANLRLVPTANALPTTLTVEPYFLHTEG
                     IYYKSRINNGYAAVVGEYVYSSSYDQGEGWASPDIGTNVTRTESMANLYPYNGAGAPA
                     PLIKINASGNALNPRQVEVRINNTLVATQTMDFFDYKKFQTTFDASLISAGTASVAVK
                     NATSISNDRMAIAQTELVYPRLFNFGGGSNFRFELEANPAGNYLEIVNFTHGGVSPVI
                     YDITNGKRYVTDITNPNLIKVVLEPSSVKRKLIMFGQYPNYPIFQVTSLQTRNFVNYL
                     TAANQGNYLIITHPVMQSSTSGANPVEEYKTYRSSAAGGSHNAKVYLIDELYDQFGLG
                     VKKSPAAIRNFILWTRARYSAPVKNIILIGKGLNYTQYRSYESNPDVERLSFIPSFGF
                     PASDNLLACDSRGDGIPKVSIGRLSTISGDEVLVYLAKVRQYEQAQAFSSPLIADRAW
                     QKNVVHVIGAADGSLGAILTADMERYRSIVADTFYGANVNTFSKVSSAPVEQASSERL
                     YKLFETGIGVMTYFGHSSATTLEFNLDNPDQYNNAGKYPITIVMGCNAGNFYNFNTLR
                     FLTKETLSEKFVLADQRGSIAFIASTHFGIVHYLDIMNTKTMGAASVSHYGKTLGEVM
                     RESVAQVYNLTTQNDYYARFHTEQATLHGDPAIRFDGSTDKPDYAIEEPLVKVNPSFI
                     SVAETHFKVDAKFVNLGKALNKNIVVEVKRTYPNLTTEVIRRDTIPGVRYMDSLSYAI
                     DIVATRDKGLNRISICLDADNTVDELYETNNCITKDIYIYEDEARPVYPYNYAIVNNP
                     ALKLVASTANPFGVLKQYVMEMDTTEFFNSPLKVSHTQSSSGGVMEFVHGMNLRDSAV
                     YHWRVAPVPASGQPSWNKSSFVYLSNSGPNSSDLGYNQSDFYQLSKSTYNRIKLDSAT
                     HDYKFGNVYQNLFLKMGTYVTSGSSSAAAFSVAINGIANIRLTSWFQSLVFNVFDPVS
                     FRPWRNVVLRAATNFPNDLGDGRFRSHAPMNLSGVLTPYNFEYRYTTPEFRKRMMDFM
                     KDSIPDGHYVVVRNFTLDPNTFGGAPNFPIAYANDWAADESLYGAGNSLYHYLKNAGL
                     SGIDSFYRPRPFGLVYKKNDPSFAPKWVFGAGVVDNPTLSVDCPTVDTVGFITSPLFG
                     PAKAWKQLKWRGSAEPAGDTTTLNVIGITADGDEMPLYTGLTPLQQDFDLSDVDAEEY
                     PFLKLQMRSSDNIHFTPYNLRYWRVTYQPVPEGAIAPNLYLNVKDTVDVGEPYQFKVG
                     FKNVSEAPFDSIKVKMVITDKNNIPHIIPQPRTRPLPINDTLQIGAVVATGTIPGANT
                     VYVEANPDNDQWEQYHFNNFAFRNLYVKPDSLNPLLDVTFDGMHILNRDIVSSKPDII
                     IKLKDEAKWMALDDTSLLTLQVRYPNGSLRRFYFNNDTLQFNPAGQVPVNDNTATLNF
                     RPYFEQDGEYELIVSGEDKSANEAGAIEYRVLFEVINKPMISNMLNYPNPFTSSTAFV
                     FTITGNEVPQNIKIEIMTVTGKIVREITKDELGPLRIGRNITEFKWDGTDQYGQKLAN
                     GIYLYRVITNLNGKALDKYKAQGDDTNKYFNKGYGKMYLMR"
     gene            complement(15142..16254)
                     /locus_tag="PLU11_03215"
     CDS             complement(15142..16254)
                     /locus_tag="PLU11_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPN58150.1"
                     /translation="MKTNRLFLLLLTTTLFQVTHAQFRKYSNEFLNIGAGARGLAMGS
                     SQVATVNDGTAGYWNPAGLVNIKDHPQVNLMHAEYFAGIGKYDYASIALPTSGNKRTI
                     GITGLRFAVDDIMNTLFLVEPDGSINYNNIQSFSSADYAFIFSFAQKLKEKANKNIDF
                     GLNAKVIHRSVGKFAKAWGFGLDAGLQIKSKNWRFGIAARDITSTFNAWSFTFTEKEK
                     EVLYLTNNEIPVKSTELTAPRVVLGIAREFKVGQKSSLLAEANIDLTFDGQRNTVISA
                     DPVSADPKLGLELNINNVFFLRGGINNFQKALYDGDTVNQKRVWIYQPSAGAGFKIQN
                     FSVDYAFTNLANQTNPLFTHVFSLRFNLVGNKNDAN"
BASE COUNT         4229 a         3945 c         3563 g         4664 t
ORIGIN      
        1 tgatattgca aaaattttaa agtgaatttt tctgctgaaa atcaagaaat agttataaag
       61 ccgggaagtc cggaaagacg gaaagtcaga aagaagccat gtcggaagtc tggtccatta
      121 caacttcccg gcatttcaga cgcccggact tcttcataaa aaaagggcgg atctttcgat
      181 ccacccttgt agctttttaa gcggcttgtt tttctttttt tctttttatc tggctcacga
      241 gcgagtcgaa agcactgtcg aaagattctt caaatgattt actggttgat tttacaaaaa
      301 agtcgtgtcg tggcacatgg acccggattt cagcaatttt gtctttaacg gaatgtacca
      361 cattgtccaa tttcaaaaac acatgtacct ggatgatgcg atcgctaaat actgtcagct
      421 tttccagctt tttgtttaca tactccatca gcttgctgtc agcattgaaa tgaacagttt
      481 gaatttttac attcatggtc atcaattttt aactgtgaaa caataaataa agaacttaaa
      541 cggaaaggtt tttcttaggg gttcggttct tatatcgtat tcaagttaca aaaaaaggaa
      601 acgtattcca ataaaataaa cccttaaaat ttattgtgct gtttccggta aatcaacccc
      661 ggggatgcgc ctttttatgg atgtctttca acttcccgat tgtgttgtga gtatacacct
      721 gcgtggccgc caggctgctg tgacccagca attccttcac cgcattcagg tcagccccgt
      781 gattcattaa atgagtggca aaactatgac gcaacacgtg tggacttttt ttatcaagcg
      841 tactggcctc ccccagtgcc tgattaacca gcgcccaggc ataccttggg tacagtttct
      901 ttcccttttc cgtaaccagc aaggtctccc cggtttgttc aaattccttt cgtttcaatt
      961 gctgatactc gcggatagcc gccatcagct ccggacttac cggaatcacc cgctctttat
     1021 tacctttccc caggaccttg atctgtcccc tcgattcatc cacctgtgtc tcctttaggc
     1081 ctgtaagctc gctcagccgc atcccggtgg cgtagaatat cgccaccagc attttcatat
     1141 tcaggctctt ccagtcctcc gcagccccgc tgagggtctg taccagctcg cgcacttcct
     1201 cttctttcac aaaaaccggt aatcttttgc ttttcttcgg actgatgacc tgccccatgg
     1261 gcgattgttc tacccctccc gtttttaact ggtatttgaa aaaggatttc agactcgaaa
     1321 ttttccggtt cagcgtgacc gcccccagcc ccttgtcttt taacgacgcc agccagctcc
     1381 ggacaatgcc ggattgtatc tcccggagtt cccggatccc gtattgtatt tcaagaaaat
     1441 caaaaaagtc ggtaagatca ttcgtatagg ccagcagggt atgcgccgaa tacctttttt
     1501 caaatttcag gtaatccagg aaggtctgta tgttggcacg ttcttgtaca ggcataaaaa
     1561 aagacagcta caaagctatt aaactttgca gctatctcaa gagtatatta tccgtcaggg
     1621 attaattagt caaacttacc agtggccagc tgctgtttgt agatggcttt cagtacttga
     1681 cccctgcgcc ttacagaagg cttaatgaaa ctctggcgct cacgcagttg caggagcact
     1741 ttggactttt cgaatttctt tttgtacttc tttaaagctt tgtcgatgtt ttcgcagtct
     1801 tttgagtcga tgatcagcat aattaaatga tacccccttc ggtatttttt taaggatggc
     1861 aaagataagt gggattattg aaatgaagaa atgaaaaaag aaatgattta acttgcctcc
     1921 atgtttacag gtattattga acacatcggc gaagttaaag aggtcatttc caatggttcc
     1981 aaccgtagtt tctggatcga atcccccatt tcagaccaac tgaaaactga tcagagtgtc
     2041 agccatagcg gggtttgcct gaccgtggaa gaggtatcgg gcaaccggca ccgtgtcacc
     2101 gccatcgatg aaaccctgaa taaaaccaac ctgggcagct gggggcccgg ctcccttgta
     2161 aaccttgaac gctgtctgca gatgaacgga agactggatg gtcacctcgt acagggacat
     2221 gtggatgcca ccggcacctg caccggtaaa acggtcaagg aaggcagctg ggaatttgaa
     2281 attgccttcc cccgcccctt tgctgaactg gtgatcgaaa aaggatccat ctgcctgaat
     2341 ggcatcagcc tgaccgcttt tgaggtaaaa aagaaacatt tcaaagtggc cattatcccc
     2401 tacacatttg aacataccaa cctgaaaaca ctcgaacccg gtcaaaccgt taacctggaa
     2461 tttgatatga ttggcaagta tctgctcaga aggttatctt tgaaagacta acactaaccc
     2521 cggtcttcct gatgaaccct gcacctgcgc acaggcaata ttaccctgca ctggacggcc
     2581 tccggggact cgccattctg ctggtgatcg tgtatcacaa tttcggcttt atcaattatt
     2641 tctttttcgg ttggctgggc gttgatttgt tcttcgtgct ttccggtttc ctgatcacgg
     2701 atattctcct gaatactgcc ggacaaccga acttcctgaa aaatttttat acccgcagac
     2761 tcttgcgcat tttcccactc tattacctgg ccttaattct ttttatttat atcatccccg
     2821 gggtgttcca aacctttcca accggatatt accgggaaaa ccagttctgg atgtggacct
     2881 atctccagaa ctggttgtat atttttaaac cggccggcga aaccaccgga ctgcatcatc
     2941 tctggtcact ggccgtggaa gaacaatttt acctgctttg gcccctgata atactgctgc
     3001 taaaaaagcc gggtcgtttg cttctcttta tttccctggt attattggcg gttatcgggc
     3061 tgcggatttg gatctggaat tcgcaggtgg aaaacctggc ctatttcaac ctctatactt
     3121 ttagccggat tgacggcctg tgtattggct ccatggtcgc cctgctgcac cggatcaacc
     3181 cgggatttct atccaaaaac acgtctgtcg tggtcctgtg ttttgccctg gctaattttg
     3241 cattttattt tatcaaccaa caatacgcat tcagttttcc ttacctggcc atcgctggtt
     3301 ataccacatt tgccatgacc ttcggtctgc tggtcaatga agctgtcacc aatggcaccc
     3361 ggctgatcac cactgttttc agctggccgg tattacggtt cttcggtaaa atttcttacg
     3421 gcctctatgt ttttcactgg ccattgtacc tgattctgaa ccccgtgctg acccaggtga
     3481 tcagtatacc tggtaacagc accctgacca gttttctagg ctccctgctg tccacgggta
     3541 tcgccattgg aatcagcctg ctgagctttc attattttga acaacctttc ctgaaactaa
     3601 aacaacggtt caactgaacc tttccacctc catggatctt gtagaatata atctgataaa
     3661 agaaaagtcg ttcaggagac atccctggga acagacccgt atgcagtttc tgttttacct
     3721 gttgaatcgt ttacccaata aaaataattt agccgatacc ggaagtggtg acgggtatct
     3781 cgcatcgggt atcgccaccc ggtaccccga agccagggta accgcgatcg atattaatta
     3841 tacagatgaa ttgctggaag cactccatac tggaaaaccg gccaatctca ggtttaccag
     3901 ggatttacat gttatccccg atgacccacc cgccgatgcc ataatcctga tggatgtcct
     3961 ggaacatatt gaaaaaccgg aagaactcct gaaagaactg ctgcagctga aagcggtgaa
     4021 cagtgaaacc cggtttatca ttacagtgcc ggcctttcaa aaactctttt ccatgcacga
     4081 taaaaatctc ggccactacc ggcgatataa cctgaaacaa ctggaaaaat tactgtcccc
     4141 gttatcgctg cggatggaaa acagtggtta tttctttaac agcctgctgc cggtcaggta
     4201 cctgcagaaa ctggttgaaa aaaataaacc ggtgaaggac aaagacagta tacacaactg
     4261 gaaaggcggt cctcttatca catggatact ccgtaccggc ttttggattg aatttaaaat
     4321 tacttggtat cttgcccgcc tcggaattaa aatacccggg ctcacctgtt attgtatatg
     4381 ccggtacgct ccataattat accctgtcat aatgaagccc tgcggctgga acccggagcc
     4441 ttttccgctt tcctccaaca atcagccggc actcatttac tgtttgtcaa tgatggcagc
     4501 acagataata cgcagcaggt actggaacag atccggaata ataatccgga ccaggtaaca
     4561 attctgtcat tgaccagcca aagtggtaaa ggggaagccg tgcgacgcgg catgatggaa
     4621 agcctgtccc tgtttcctgc tgttacggtt tccggttata tggatgcgga cttatccgtc
     4681 agcctggatg aaatgacccg tctcttcgga tgcttcagcc atgcagagaa acgtttcatt
     4741 ctgggaagcc gggttaaaaa aatcggtgca cagatcagcc ggaatgaatg gcggcacttt
     4801 tacagccgta ttattgccac tattgtgggt tccatcatcc ggctggatgt atacgatacc
     4861 caatgcagtg ccaagctgtt tcgccaggat agtattgaaa ctgttttcaa caaaccattc
     4921 agaacaaaat ggttgtttga tgtggagatt atctgccgtt tgcacaaggc ctatggaccg
     4981 ttaaatgata acgggctgga agaacccctg cttgaatgga cggaacgaaa gggttccaaa
     5041 ctccgctggt acagtatctt ccgggtaatc cgtgaagtct tcattttaaa gaaatactat
     5101 accggcaaat gactgaatcc ggaaataata aaaaatacca ggactacctg tttattatcg
     5161 ttctggccct gttggcatac tggccggttt cctttatgct gttcagcgta aagaacgacg
     5221 cgatcaatta tttcctggcg atgcgctaca ataccagcga agccattcaa tccgggcatt
     5281 tccctttatg gtctccctat attaacctcg gctatccgct ccatggcgat atgcaatccg
     5341 gcacctggaa tccggtggta ctggtgatga gccttgtccg taagtatgat atatactggc
     5401 tccaactgga aactatcatc accataatta tttcaggatg cagtttctat aagttgttat
     5461 cgcatttcca actcagccgg gtcatcttat tggctggcag cgcggcctat atgctcaatg
     5521 gttatataac ggatgcgggt cagttcctga actggctcta tgcggccgct tacctgcccc
     5581 tggtagtact ctatgctatc cggagttttg aacagttcag cacccgggat gcctttttac
     5641 tgggagtcag tttttcactg atgttattat ctgcttaccc tgccgatttt attttattgg
     5701 gatacttact gacggcctat ttcctgttct ccgttttccg ttataaaaaa gattcgggcc
     5761 tgattaaaag ttttacacgt tgtttgaaac cagcactgat tgctgtcgtg agttttgcgt
     5821 tgatttgcct gccagctatt ctctcctacc tgccctttct ggcttccatc cggcggggaa
     5881 acggcgtgga attggatgtc gcgttgtcca attcattttc gcccgtcaat tttatttcag
     5941 ttttaacccc ctggcccacc caacggctta actgggaaca atatacggat ccgctgatcc
     6001 gtaacagtta catgggcctg attcccctgg tgtttcttat tacgtatttc cggcaaaaaa
     6061 gcaaaaaaac atttgttcag tatttcttta ccggcaccct gattttcttc ctcctgttta
     6121 gcctgggtaa agccggagga ctgagggtac tcagttatta cacattgccg ctgttggata
     6181 gtttccgtca cccggccaat gcaaaactct tcgttctctt tgccgcccag ttattgtcct
     6241 ttcttacact tgataagtac ctgaaagagc cgggttcttt acagccggcc atcaagcgga
     6301 tcgtgaatat tttactggtg ataaccggtc tcttactgat caccggactt tttaagaccc
     6361 ggatattcca actggcaggg aagctgaatg taacacatgg acgtttagac cttaaaacca
     6421 tatttgatgc tattagcttt tatgacttcc tgtttctaaa cgcaaccctg gcattcatca
     6481 tactccttat gctgcgttgg actttaaaga agaaccgtct gcagcgatgg ttgttgccgg
     6541 tgctctgtgc cgaaatgata ctggctgcac aattcatgct cccattgacc tatttcagga
     6601 cttcttcacc acaaacggta caaaatatac tgaacgcaca accgaaaggt tatccggctc
     6661 ctgacgggtt tacttctata cgtgagatgt ccgcagatgg aatgaattat tttaatgaaa
     6721 tcggttgcct caacccgttt aataaaaagc cgggacgctc cgattatatt atcaccccgg
     6781 ccaatctttc ctggcaactg gatttctggg aggacacggc tttccgtgaa aaaataattg
     6841 aatacccttt ctattattta gcggatacta tttaccaggt tcctgataaa aaagatttta
     6901 taagggatac cctgtcccgg aaagctgcat tagccaatct gtctttatat gcaaaaccgc
     6961 tgattcctga taaccaggat gccctatccg tcattgaatc attcggcccc gaacatattc
     7021 gctttaaagt aaattcagcc ggaccaactt tccttgtttt atttcagaat cattacccga
     7081 attgggccgt cagaataaat ggtcaaaaaa cggatatcat tccaaccaat catgcattta
     7141 tgggggtggt ggttccgggt ggggaacaag tagtggagtt ccggtatgaa gcccccggta
     7201 ttctatacat ggccatgggt tcactactct ttactcttgc cggtttagtg tactttagcc
     7261 ggaagaaaaa aaatacccgt gaacagaaag ctgactaata cgattcgttt tgtaatggat
     7321 gaaggcctcc ctgcttttat ccgcgacagc cgctggttca tgtacccttt cttctggtat
     7381 tggttcaagg gaaagaacct ggctacttat atggacttta agaagattgc ctggggttta
     7441 tcagaagccg ctttcaaccg cgtataccag gaactggatt gcagggccac cgaccgccct
     7501 accgacctga atgagaaatg tatccggctt atgctggaaa aacttcatcc cgaagctaaa
     7561 actatgctgg atgtaggttg cggaagagga cattggcttt cattagccgg cggggcccgg
     7621 aaagatctgc tgcttaccgg ctgtgatgta ttcgaagaat ccaccgttaa taaatccttt
     7681 actttcaaac agggttcaat ctaccagctg ccatttgagg accggtcttt tgatattgtc
     7741 acctgccatc atacgatcga gcatttgccc gatatccata aagccatatc agaattgaaa
     7801 agagtaaccc gtaaacaact gattattgtt acaccccggc aacgttactt ctactatacc
     7861 ctggaccttc acctgcattt cttccctgaa aaggaatacc tgcaacaggt caccggcata
     7921 cccaacggtg attatatgga ttgcgacgga gactggtgcc tgatcgccaa taccggttct
     7981 atttaatgtg tttatctttt aaagcagccg ccacggccgt atccatgagc ctttctgcat
     8041 agggtcttga aatgtcattt aatttttcga cccccgcctg tatggcatcc ttggttccct
     8101 gctcccgcag acgggtcagc tcttccatgg ctttttcagt ttcggcaagt tccatgtccg
     8161 tgaggaaacc ggtatttcgc tgaataaact ggcgggtggt ttccagaatc tgcaaggctt
     8221 ctgtctgtgc ttccaccaat gcccgggtgc ggatatcttc ctttgcattc gtgatagagt
     8281 ccatcagcat tttctccact tccgcatctg aaagtccata ctgcggtttc acgtcaatac
     8341 tttgttccac cccactccgt agttcttttg cttttacagt cagaatgcca tccgcgttca
     8401 ccaggaatga aacctctact ttaggtaatc cggccggcat tcccggaatg cccgtcaggt
     8461 taaactcagc cagtttacgg ttgtctttga ccaggtctct ctcgccctga aatacactga
     8521 tgcgcatact tccctggcca tctttctgtg tggtgtattg ccttccggct ttggatggta
     8581 tttttgaatt gcggggaatc aatacatcca tcaatccgcc catggtctcg atacccaggg
     8641 aaaggggtgt gatatcaagt aataacaggt ctttattatt tccggccaat acatcggcct
     8701 gtaccgccgc accaagcgcc accacttcgt cgggattcaa ggtatcatgt actttttttc
     8761 caaaaaaagc actgactctt tgtttcacta aaggcacccg ggtggaaccg cccaccatca
     8821 ccacttcatc aatatcactg gttgcaagac cggcatcttt catggcaccg gcacaactgg
     8881 taatagtgcg gtcaatcaat ggcgtaatca gttgttccaa ctgttccctg ctcagactga
     8941 actcctgttc cgccacctcc ccgatataat tgtcgctgct gctcaaggca attttggctg
     9001 tttccgccag gagccggagt tcctgtgaaa gatttttatg catcttcagt tcctcatcag
     9061 taataccagc caaagatgcc caatactgta caatcgcccg gtcaatatca tcgccaccca
     9121 ggtaagtatc cccattcgtg gagagtactt cgaaaatgcc attactgatc cgaagtatgg
     9181 atatatcaaa tgttccacca cccaggtcat acacggcaat cgtctttatt tcatcccgac
     9241 ttaacccaag cccgtaagcc aggctggcgg cggtaggttc gttcacaata cggagcacat
     9301 ccagacccgc cagtttgccc gcatcacggg ttgcctgccg ctgggcatca ttaaaataag
     9361 ccggcacggt gattacggct ttcgtaaccg gtgttttcag tatatgttcc gctcttgctt
     9421 taagttcctt cagaatcagg gaagataatt ccacaggcga gtagaagacc tctcccacct
     9481 gaattttcac gaggctttct gtatggtcat caatcacttt ataactaaag taaccgctgt
     9541 gttcccggat atcattgtaa gattttccca tgagccgctt cacagaaaaa atggtgtgct
     9601 gaggctcggt gatcaggaat tttttggctt cttctcctac agtcacctgt cccgtttcgc
     9661 cgaaatgaac aatggaagga accagggaaa cgccgtcatg ttcttttaat acaaccggtt
     9721 gccgggtatc cgggtgtatg atcgcgacca ggctgttggt ggtacccagg tcgattccga
     9781 caatcatttc atgctgttgc agacttcccg tggctatgtt aattcctatt ttggccataa
     9841 ttgatagtaa ttcttttgtg atcttatcgc agggcaggcc cctggctggg ataaccatgt
     9901 atccaatctg caaaattcgc ttatcccggg cgtaaaaaaa agtcccgccg gggcgggact
     9961 tttcgttttt tggaatattt tctaacgcat caggtacatt ttgccgtacc ctttgttgaa
    10021 atacttattg gtatcatcgc cttgcgcctt gtatttatca agggctttgc cattcaggtt
    10081 ggtaatcacc cggtagagat agataccatt tgccagtttc tggccatact ggtcagtacc
    10141 atcccatttg aactctgtga tattacgtcc aatgcggagt ggcccaagtt cgtccttggt
    10201 gatttcacgt acaatcttac ccgtcactgt catgatctcg attttgatat tctgcggcac
    10261 ttcattaccg gtaatggtaa atacaaaggc cgtggaactg gtgaacggat tcgggtagtt
    10321 gagcatattg gagatcatcg gcttattgat cacctcaaat aatacacggt attctatggc
    10381 accggcttca ttggcactct tatcttcccc tgaaacaatc aattcatatt ccccatcctg
    10441 ctcaaaataa ggccggaagt tgagcgtggc ggtattgtca ttgaccggca cctgtcctgc
    10501 tggattaaat tgcagggtgt cgttattgaa atagaagcgg cgcaggctgc cattgggata
    10561 acgtacctgt aatgtcagca ggctggtatc atccaatgcc atccatttag cttcatcctt
    10621 caacttaata ataatgtccg gtttggagga tacaatatcg cggttcagga tatgcatccc
    10681 gtcaaatgtt acatccagta aaggattcag gctatctggt tttacgtaaa gattacggaa
    10741 agcaaaatta ttaaaatgat actgttccca ctggtcatta tccgggttag cttccacata
    10801 taccgtattg gcgccaggta tggttccggt ggccaccacc gcaccgatct gcaacgtatc
    10861 attaatcggc agaggccggg ttcttggctg gggtataata tgcggaatgt tatttttatc
    10921 ggttattacc atcttcacct tgatactgtc aaatggcgct tcactcacat tcttaaaccc
    10981 gactttaaac tggtaaggtt cacccacatc caccgtgtct ttcacattca ggtaaagatt
    11041 tggcgcaatg gctccttccg gaacgggttg ataagtcact ctccaataac gcaggttgta
    11101 tggggtgaaa tggatattgt ctgaactacg catctggagt ttcaggaagg gatattcttc
    11161 cgcatctaca tcagacaggt caaaatcctg ctgcaggggt gtcagtccgg tatacaatgg
    11221 catttcgtcg ccatctgctg taatcccaat tacatttaaa gtggtggtat cgccggccgg
    11281 ttcagcgctt cctctccatt tcagttgttt ccaggccttg gccggaccaa ataaagggga
    11341 agtgataaat cctacggtat cgacggtcgg acaatcaaca gaaagagttg gattgtccac
    11401 cacacctgct ccaaataccc atttgggagc aaacgacggg tcattctttt tataaactaa
    11461 accgaaaggt ctgggacgat agaatgaatc aatacccgac aatccggcat ttttaagata
    11521 atgataaagg gaatttcccg caccgtacag cgattcatcc gccgcccaat cattcgcata
    11581 agcaattggg aaattgggcg ccccgccaaa agtattcgga tccagggtga agtttctgac
    11641 gacgacatag tgcccatcag gaatagagtc tttcatgaaa tccatcatgc gcttcctgaa
    11701 ttccggggtg gtataacggt attcgaaatt gtatggagta agtacaccag aaagattcat
    11761 aggagcatga cttctgaaac gcccgtcacc caggtcattc gggaaattag tagctgccct
    11821 gagaacaaca ttcctccatg gtctgaagct cacggggtca aaaacattaa acaccaacga
    11881 ctggaaccag gaggttagcc tgatattggc aataccattt atcgcaacag aaaaggcggc
    11941 agcactggat gatccactgg tcacgtaggt acccattttc aggaagagat tctggtatac
    12001 attcccgaat ttatagtcat gggtggcaga atccagtttt atcctgttgt aagttgattt
    12061 gctcaattgg taaaaatcag attgattata tccaagatcg ctgctgtttg gacccgagtt
    12121 gctgagataa acaaaagatg atttattcca ggaaggctgc ccactggcag gcacaggcgc
    12181 cactctccaa tggtatacgg cactgtcacg caggttcatt ccatgtacaa attccataac
    12241 cccacctgaa gaactctgcg tatgggatac cttgagcggt gaattgaaaa actcagtggt
    12301 gtccatttcc atgacatact gtttcaatac gccaaaagga ttggccgtgg atgccaccag
    12361 ctttaatgcg gggttgttta caatggcata attatatgga tataccggac gggcttcgtc
    12421 ttcatagatg tagatatcct tggtgataca gttattggtt tcatacaatt catctactgt
    12481 attatctgca tccaggcata tagaaatacg attgagccct ttatcccggg ttgccacaat
    12541 atcgatagca taagacaatg aatccatgta tcgtacaccc ggaattgtat cccggcggat
    12601 cacttcagtg gtcaggttgg gataggtccg cttcacttcc actacaatat ttttattcaa
    12661 cgccttaccc agattgacaa atttcgcatc tactttaaaa tgtgtttctg ctacagatat
    12721 aaaactggga ttcactttta ccagcggttc ttctatcgca taatccggtt tatcggtaga
    12781 cccgtcaaaa cgaatcgccg ggtcaccatg caaagtggcc tgttcggtat ggaaccgggc
    12841 ataataatca ttttgggtag tgaggttgta tacctgggct acggattcac gcatcacttc
    12901 tcccagtgtc tttccataat gtgaaaccga tgccgccccc atcgttttgg tattcatgat
    12961 atcgagataa tgcacaatac cgaagtgcgt actggcaatg aaagcgatgg atccccgctg
    13021 gtccgccagt acaaattttt ccgaaagggt ttctttggtt aagaaacgaa gtgtgttgaa
    13081 attatagaaa ttaccggcat tacaacccat tacaatggta atcgggtatt tgcctgcatt
    13141 attatactga tcaggattat caaggttgaa ctcaagcgtg gtggcggaag agtgaccgaa
    13201 ataagtcatc accccgatac ccgtttcaaa cagcttgtac agacgttcac tgctggcttg
    13261 ttccaccgga gcagaagaca ctttactgaa ggtattcaca tttgcaccat agaaggtatc
    13321 ggcaacaatt gaccggtaac gctccatatc tgctgtaagg atcgctccca gcgaaccatc
    13381 agcggctccg attacatgca ctacattttt ctgccaggcc ctgtctgcaa tcagcggact
    13441 ggagaaagcc tgagcctgtt catactgcct tactttagca aggtaaacca gtacttcatc
    13501 acctgaaatg gtcgacaggc ggccgatcga tacttttgga ataccgtccc ctcttgaatc
    13561 acatgcaagt aagttatcgg aagcaggaaa tccaaaagaa ggtataaacg aaagtcgctc
    13621 cacatccggg tttgattcat aactccggta ctgtgtataa ttcaggcctt taccgatcaa
    13681 tataatattc ttcaccgggg cactgtaacg ggcacgtgtc caaagaatga aattccggat
    13741 agcggccggg ctttttttaa cgcccagtcc aaactggtca tacagttcat caatcaggta
    13801 cactttggca ttatgacttc caccggcggc agaactacgg taggttttat attcttccac
    13861 cggattggcc cctgaggtag aggactgcat aacagggtga gtaatgatca ggtaatttcc
    13921 ctggttggca gcagtaagat aattaacaaa attccgggtt tgcaggctgg tcacctggaa
    13981 aatcggataa ttaggatact gaccaaacat gatcagtttc cgctttacgg aggaaggctc
    14041 caatactact ttaatcaggt ttggattggt gatatcggtt acataccttt tcccattggt
    14101 aatgtcatag ataacaggac tgactcctcc atgggtgaaa tttacgatct ctaaatagtt
    14161 accggctggg ttggcttcca gttcaaaacg gaaattactc ccgccgccaa aattgaacaa
    14221 gcggggataa accagttcgg tctgtgcaat cgccatccgg tcattggaaa tactggttgc
    14281 attctttacc gctactgaag ctgtgcctgc actgatcagc gaagcatcaa aagtggtttg
    14341 aaattttttg tagtcaaaga aatccatggt ctgggtggcc accagggtat tatttatcct
    14401 gacttccacc tggcgtggat tgagtgcatt tccagaagca ttgatcttta ttaaaggggc
    14461 cggggcaccc gcaccattat aggggtacag gttagccatg ctttcagtac gtgttacatt
    14521 cgtaccaata tccggagaag cccatccttc accctgatca taggaagaag aatacacata
    14581 ctcccctaca accgcggcat atccattatt aatcctgctc ttataataaa ttccctctgt
    14641 atgaagaaaa tatggctcca cagttaaagt ggtaggcagc gcattagcag tcggaaccag
    14701 ccggagattg gcactggccg cattaatagt cagaaagtag gaaactgtat ctgtttcaag
    14761 actccatttg tcattcagct ggtaatccgc attccggtac aattcattat ccgatttacc
    14821 atcattcatt tctccccaga actcaagata atcacccgcg cctaaggtac cggtctgcac
    14881 cgttgtgtac atgggcacct gttctccctt tctccataat tgaaagtcct gggcaggggt
    14941 gttaccaata cccagagaag ccagcacagg ctgactgatc cggtaaagtc cgttaacacc
    15001 tactttaaat ttataatagg ttttggaata gtcaatccat tcattattgt aggtctgcga
    15061 ctgggcagcc agaccggaaa taagaagtag cagaagtaaa agtcttttca tggatcaata
    15121 atttatcggg tactgacaga tttaattagc atcgtttttg tttcccacca ggttgaaccg
    15181 gagtgaaaaa acatgggtaa ataacggatt ggtctggttg gccaggttgg taaaggcata
    15241 atcaaccgaa aagttctgga ttttaaaacc agctccggca ctcggctggt aaatccagac
    15301 ccgcttttga ttcaccgtat ccccgtcata cagggctttc tggaaattgt tgataccacc
    15361 gcggaggaag aaaacattat tgatattcag ctccagtccc agtttaggat cagcacttac
    15421 cggatcggca ctgatcaccg tattccgctg tccgtcaaat gtgaggtcaa tattagcttc
    15481 tgccagtaaa ctggatttct gtcccacttt aaattcacgg gcgataccca gcacaaccct
    15541 gggtgccgtt aattcggtgg acttaaccgg tatctcatta ttggtcagat agagtacttc
    15601 tttttctttt tctgtaaagg tgaaagacca ggcattaaat gtgctggtaa tatcacgggc
    15661 cgctatacca aagcgccagt tcttgctttt gatttgcaga ccggcatcca ggccaaaacc
    15721 ccaggctttg gcaaacttgc ccacactgcg gtgaatcact ttggcattca gtccaaagtc
    15781 aatattctta tttgctttct cttttaattt ctgggcaaag gaaaaaataa aggcataatc
    15841 tgcggaagag aaagactgga tattgttgta attaatggat ccatcgggtt ccaccaggaa
    15901 taaggtgttc atgatatcgt ccaccgcaaa acgtaaaccg gtgataccga tggttcgctt
    15961 atttcctgaa gtaggaaggg caatgcttgc ataatcatac ttaccgatgc cggcaaaata
    16021 ttcagcatgc atgaggttta cttgtggatg atctttgatg ttgaccagac cggcggggtt
    16081 ccagtagccg gcagtaccat cattcacggt ggcgacctga gagctgccca tggccaaccc
    16141 tcgtgcacca gccccgatat tcaggaattc attcgaatat ttcctgaatt gagcatgagt
    16201 aacctgaaac agcgtggtgg taagcagtag caggaataag cggttcgttt tcattcactg
    16261 atttttcaaa ataagcaatg gattaaatta cttgttttcg attaggtatt ggatagcttg
    16321 taaaattaat ctttaccctt ctaaaatcat aacattgtgg agggtttaga ctaatcattg
    16381 aaaacgaata ttttacatga a
//
LOCUS       DAOPMW010000034        16338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3481_length_16338_cov_8.150587, whole genome shotgun sequence.
ACCESSION   DAOPMW010000034 DAOPMW010000000
VERSION     DAOPMW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16338
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_16338_cov_8.150587"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1515
                     /locus_tag="PLU11_03220"
     CDS             151..1515
                     /locus_tag="PLU11_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN58151.1"
                     /translation="MKINLVSLMLAILPATYIHLPAQGIKISSGASLVVNGSANLVLA
                     NTGITNNGQFFPGNGAVMFTGNSATSGSFINGSSTTGFYNLTLNKTSNGIQLGRDISI
                     SNILLFTSGDSLFLNSFNIDLGLTGTLSGEASTKRITGRTGGYIQSTQVLNAPAAVNP
                     GNLGFQITSTANLGSTVIRRGHQQQSGASIYRYFTVTPTNNSGLAATIEFYYFDNELA
                     GIAEPNLALFSSANGGVQWLNLGEDDINQGANILTLASIDILNRFTLANISAPLAVKL
                     IRFYVVPAANEVTLHWLTSTESNNHYFAVERSADGMHFMEIGKVPGYGTSNQEQQYRF
                     TDLWPLSDKSWYRLRQVDIDGKISYTPVLMINRNFNTIQPARLYPNPVPGTTIFLSLN
                     SDKAGERTFMMYNQAGMLLKQFNVSLVTGTQLIELQTGLLPAGIYILRQQGNARFNLN
                     FLKQ"
     gene            1819..2259
                     /locus_tag="PLU11_03225"
     CDS             1819..2259
                     /locus_tag="PLU11_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPN58152.1"
                     /translation="MGETWNKKERQKKKQKEKMDKAEKMKERKEQTGKKSLEDMMAYV
                     DENGNISATPPDPRKRKEIKLEDITIGVPEYVPDAEDEFRTGKVSFFNHEKGFGFIRD
                     LVTQESIFVHINNLSGPVQENDKVSFTTEKGPRGLMAVNVKLVP"
     gene            complement(2271..2522)
                     /locus_tag="PLU11_03230"
     CDS             complement(2271..2522)
                     /locus_tag="PLU11_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HPN58153.1"
                     /translation="MTSEQIEKYIAPGKRKDNPLNIHFKDRQPVTGIFIQESDYEELK
                     SKNLWRVVSGNNVKEWEQRKDLNLSRIFNGISFTRLSEV"
     gene            complement(2601..2852)
                     /locus_tag="PLU11_03235"
     CDS             complement(2601..2852)
                     /locus_tag="PLU11_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPN58154.1"
                     /translation="MTIYVSNLSFTMTDDQLKKVFVPFGEVSSARIMTDKFTNRSRGF
                     AFIEMPDELEAQKAINDLNGSPCEGRPMVVNMAKPRQQF"
     gene            3146..3409
                     /locus_tag="PLU11_03240"
     CDS             3146..3409
                     /locus_tag="PLU11_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58155.1"
                     /translation="MQDHYSLLHTIYEIVKNDPQPERYSCKPRELILRRMQEWSDIQQ
                     QLHQLEKEELVTTEQQETMVIRITKAGLSLVKEQLEIARLSGN"
     gene            complement(3411..4283)
                     /locus_tag="PLU11_03245"
     CDS             complement(3411..4283)
                     /locus_tag="PLU11_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPN58156.1"
                     /translation="MLRTKLHKAGFIITSFMLMAAASGNKKITLAEQKLNTVSVPADS
                     LVISKHMFDTAGLCICLPDLTAAELDAAPRIRLQEEARVFVQAHLRENAFYYDKIKAK
                     SGPVFSMIDQVFTKYQVPVEMKYLAIVESKLDSRIVSSAGAAGLWQFMPVAAKSFGLR
                     ISGKQDDRRNEYKSTVAAAKCLLYLNGIFDDWLLTLAAYNSGPAKVLSAIRKSGSRNY
                     WELEKWLPAETRKHVKKFISVHYHFEGHGSVATMTKPETLAHTNDVALFLNRTKQSPD
                     SVNVEGEKISSKLK"
     gene            complement(4725..5141)
                     /locus_tag="PLU11_03250"
     CDS             complement(4725..5141)
                     /locus_tag="PLU11_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58157.1"
                     /translation="MKKIILNNRSIAIALLTLFTIALSPVVNATEKKPVIAAEIKFVG
                     KVQNNPVFELTVPGAGVQEEYIVSVRDEHGNVLHIETIKAAGFTKKFMLSLDENNDGA
                     NYSIQFVISTKSNKKSALYSVNNNNRNVEEVVVTKL"
     gene            5736..6326
                     /locus_tag="PLU11_03255"
     CDS             5736..6326
                     /locus_tag="PLU11_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPN58158.1"
                     /translation="MAFKKDDVIIIEPSEIFVGKKDAPVTLVEFGEYESEECAKVNEV
                     VKQLLEEYEGKIRFQFRHFPLTLIHQRSLKASESAVAAAQEGKFWEMHNVLFNNRRNL
                     GTTSLKLHSKEAGVKNKKFLDDLVNGMYGWQVQDDIKEGHNRGVKELPTFFINDVLFV
                     GKPTFANLSAAIDSALKKGKSKAPVKKAAVKQKQRA"
     gene            complement(6701..7009)
                     /locus_tag="PLU11_03260"
     CDS             complement(6701..7009)
                     /locus_tag="PLU11_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPN58159.1"
                     /translation="MNIYVSNLSFAVVDEDLRSYFAEYGDVTSAKVIMDKFTNRSKGF
                     GFVEMSDDAAANKAIAELDGATVDGRSIKVSVAKPREERPARSNNSRPSYSGNNNNRW
                     "
     gene            complement(7404..9803)
                     /locus_tag="PLU11_03265"
     CDS             complement(7404..9803)
                     /locus_tag="PLU11_03265"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58160.1"
                     /translation="MKQHTSLILILCFLFSSTGILYAQDVPVTIRLQNSKKEPVPFAS
                     VSAINRMDSTLAKQQVADSNGLVRFPLIKGQQYRIEVVALNYAPFEKNISVGSGTPDF
                     RFTMEETGKTMEGVVLRSSKPLMRQEDDKTIVDPENLVAASSSGYEVIEKTPGLFVDQ
                     DGNIYISSLSPASVQINGREMRMSAADVASILKSLPPTAISKIEIVRTPSAKYDANSS
                     GGVVNVVLKKGVKIGLTGSVNAGIQQGNYGNQLIGFNLNNNDGEKSLSLNFNYSHRNS
                     YEKIVTDRIFAPDSVLGQEAYTTYPGNTFFGSYVYSWGKKKWEFELSGTANLNLSDNK
                     TDNQNRIEKISTNQLLTNNLNQVTNQGNSLVIGNGFESKLKLDSLGSEWTSQFFYYNS
                     NNKNNQGYDTRYYTPIPFTNSGDGTMDNHRDLLSGRTDLKLKMKNRLTLETGLKSTLH
                     LFNNNTLFYTESNGNRVPDAGRTNRFRYKENINSAYAQGSKTMGKDIVLKFGTRLENT
                     NMRGNQEIPTDTSFSIHRTDLFPYVFLSKSLMKIAGYDLRAYLVYRRTITRPVYEQLN
                     PFSRYVDAYLSEVGNPGLRPQFTRNYEANISVDERPILAIGVNDTKDIFSNVVYQSDT
                     SQSQAYRTYDNLGKNKEWYFRALGALPPGGKYFFVLGTQYNHNFYEGIYENAPLSFKR
                     GTWTFFTYQTLKLDKLSVITLNGFWRLKGQQQFYELGSFGALNASINRRFMKEKLIIT
                     LSVSDMFLTNKNDFSLKQGTVDAIGFRQADTRRVGINIRYSFGMRKKEEGVNMFNVEP
                     Q"
     gene            complement(9892..10353)
                     /locus_tag="PLU11_03270"
     CDS             complement(9892..10353)
                     /locus_tag="PLU11_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HPN58161.1"
                     /translation="MKSSTLPFFERFAVKVTKATGRPVAFLMACLVILIWIFTGPVFK
                     FSDTWQLVINTGTTIITFLMVFVIQHAQNKDSVALQLKLNELIAASSRASNRLVDVED
                     LSAEELETLKKFYIKLSELAKLEKNIHRTHSIDEAENTHQDKIRTQDESKR"
     gene            10418..10918
                     /locus_tag="PLU11_03275"
     CDS             10418..10918
                     /locus_tag="PLU11_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPN58162.1"
                     /translation="MVRFTVTIKKFGSKGEKTGWSYFEVPADIAQELKPGNKKEFKVK
                     GKLDKFTIKRTSLIPMGGGLFIMALNADMRKAIRKKEGAMLVVQLTEDKSDFVFNADF
                     IECLKDEPPAHSFFQSLTGSHQRYFSKWIDSAKTDATKTKRIAMAVNALARSLGYPEM
                     IRSSRQ"
     gene            complement(11084..12238)
                     /locus_tag="PLU11_03280"
     CDS             complement(11084..12238)
                     /locus_tag="PLU11_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPN58163.1"
                     /translation="MYRPKTLAIIGLFALFIFLACKKETSTPANDPVSAVLNLPANPF
                     NYASISLPGYLNSPPIQGQINTPATNPITNDGATLGRVLFYDKNLSANNTISCASCHK
                     PELGFSDTAFKSKGFNGGLTGRNSMSLINARFYTPGSFFWDQRAATLEAQVVAPIQDL
                     VEMGMTLPALENKLNALAYYPPLFKKAFGNETINSDLISKALSQFVRSIVSYQSKYDA
                     GRASLPANPAPAPNAVFPNFTAQENRGKELFLTPANGCAACHGTETFVAPTEKNNGID
                     MTTVDRGFGAVSNNTNQDATFKVTTLRNVELTAPYMHDGRFKTLEEVVEHYSTGVKNH
                     PNLSPQMKLPNGQPRFLNLSAPDKAALVAFLKTLTDRTVQTNQLYTNPFK"
     gene            complement(12422..12940)
                     /locus_tag="PLU11_03285"
     CDS             complement(12422..12940)
                     /locus_tag="PLU11_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN58164.1"
                     /translation="MKKTILPLALAILLFACQQPNTATDPATAIKEIGNTLDSFNRAA
                     ARADFNAYFNYYTEDGIFCGTDATERWDKKQFMEWSKPYFDKGKAWDFTAIDRHIYLD
                     PAGDLAWFDELLSTQMKICRGSGVIVKQNKEWKLKQYVLSTTVPNSLVDTVTIMKTAE
                     EDSLIRVITKSK"
     gene            complement(13035..13649)
                     /locus_tag="PLU11_03290"
     CDS             complement(13035..13649)
                     /locus_tag="PLU11_03290"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HPN58165.1"
                     /translation="MKKTILLLSILAVVIFLTNCNTTGEKKKGIPTASTDTISQIKSP
                     LETGQEMAMKTKAILGKHLVTAIQDMGTEGAIEFCSSRAIQLTDSMSTVLGSRIKRVA
                     ERNRNPVNVASADELSYIKEIKASLTRKENPKPQLKETTETYTAYYPILTDQLCLQCH
                     GDKKTDIKPATLSTLNRLYPGDKATGFKLNELRGIWVVEMKKAK"
     gene            complement(13729..14103)
                     /locus_tag="PLU11_03295"
     CDS             complement(13729..14103)
                     /locus_tag="PLU11_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58166.1"
                     /translation="MYFKVLFISQANHMILFRYLKQTFQSPSLAKNMLLGAGIGLALI
                     GLFLFKADEPNPEWHELWMIRPFVIVPFAGAMGGLFFTFMEPWRSKAGWLKATAYFLC
                     LIVFLIGLFIGTVLGLDGTYWD"
     gene            14157..14915
                     /locus_tag="PLU11_03300"
     CDS             14157..14915
                     /locus_tag="PLU11_03300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HPN58167.1"
                     /translation="MAGFVKEDSIVRRIWGHSDTVLFIFAGASAEFALNKAVDWLYFT
                     GKLPADPLGRLFSTVGYAKKIVFAPEAEAQQAIGRMRQIHGAVEQNRGSAIPEWAYRD
                     VLYMLIFYSIASYELLERKLSEDEKEEVYNVFYRLGSGMGLKGLPTTYTTWLPDREAH
                     LRSDLQRSQYTADLFRQYKKHLGTFRYKLLIEAQKMLLPDTVKEMLQFKTIRWLYPAV
                     PVYKFSRMMRLDSIIKNVLLPGKYKQEIRGLDVG"
     gene            14968..>16338
                     /locus_tag="PLU11_03305"
     CDS             14968..>16338
                     /locus_tag="PLU11_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPN58168.1"
                     /translation="MISSINDFKNPFFIGVAGSGMSALAQYLKGIGKNVNGSDRFFAP
                     GVYNETQEKLEAEGIRCFVQDGSGITDATDLVVCSTAIEDTVMEVQKAKQLNIPILRR
                     SELLSLIAGSKKTIAVGGTSGKSTTSAMLFDILEKAGLEPSIISGAGLISIIKEGKIG
                     NAKVGAGELLVIEADESDGSIVQYKPEIGLLMNIDKDHQEIDELLSLFGTFKNNSRKF
                     ITNQSHPLTAPLSSQWANDFATDTSHDAGYAASDFKQVGMEISFKVNNQSFSLHLPGK
                     HNMENAVAATAVAHQLGVDLATCAAALKEYQGIYRRHQVLGQKNGVWMIDDYAHNPVK
                     CAAAIAACQPIAPKVVAWFQPHGYKPTKFLRADFVKELAAVLRPQDEIWMSEIFYAGG
                     TAVKDISANDLINDLKALGKKAFFVEDRNDFIDTARPHLDSNTVLLLMGARDPSLEQF
                     AKESWER"
BASE COUNT         4495 a         3518 c         3767 g         4558 t
ORIGIN      
        1 atccacctga taatgaaaat caacaaagag tcctccgaca ctgctgatga tacgtataac
       61 gccgatgccg gaggactctt tgttgatttt cattatcagg tggatagctg gggtagcaga
      121 agtacataca ccaaataaat aagccgcact atgaaaatca atctagtcag tttgatgctg
      181 gcaatattgc cggccactta catacatctg cctgcacagg gtataaagat cagttccggg
      241 gcttcactgg ttgtgaatgg atcggcaaac ctggtactcg caaatacggg gataaccaat
      301 aacggtcagt ttttcccagg taacggagcc gtaatgttta caggtaattc ggccacatcg
      361 ggatccttta ttaatggcag ttcaactacc ggattttata atctgacact gaacaaaacc
      421 agtaatggta ttcaactggg tcgagatatc agtatatcca acatccttct ttttaccagt
      481 ggggatagtt tatttctgaa tagcttcaat attgatctgg gtttaacggg tactttgagc
      541 ggagaagcga gtacaaagcg cattaccggc cgcacagggg gatatataca gtccacccag
      601 gtattaaatg caccggcagc cgtaaatccg ggtaacctgg gatttcagat aaccagcaca
      661 gctaacctgg gaagtaccgt catccgaaga ggtcatcagc aacaatccgg ggcctctatt
      721 taccgttatt ttaccgttac accaaccaat aacagtggat tagcggccac cattgaattt
      781 tactacttcg ataatgaact ggccggaata gccgaaccca acctggcact gttttcaagt
      841 gccaatgggg gtgtgcagtg gctaaatctg ggtgaagacg atattaacca gggtgccaat
      901 atactcacac tggcaagcat agatatattg aaccggttca cgttggccaa tatcagtgcg
      961 ccattggctg taaaattgat caggttttat gttgtaccgg ctgcaaacga agtgacgctt
     1021 cattggttaa ccagtacgga atcaaacaat cactattttg ccgttgagcg atccgctgac
     1081 ggaatgcatt tcatggaaat aggcaaggta cccggctatg gcacatctaa ccaggaacag
     1141 caataccggt ttactgacct ttggccctta tctgataaat cctggtaccg tctgcgccag
     1201 gtggatattg acgggaaaat aagttatacc ccggtgctga tgatcaacag gaatttcaat
     1261 actattcagc cggcaaggct ttatcctaac ccggtaccag gcacaacgat cttcctgagt
     1321 ttaaatagtg ataaagcagg agaacggact tttatgatgt ataaccaggc aggtatgctt
     1381 ttgaaacaat ttaatgtcag cctggttact ggtacacaat taattgaact ccaaaccggt
     1441 ctgcttccgg caggtattta tatcctcagg caacaaggaa atgcaagatt taatttgaat
     1501 tttttaaaac aatagtgtac gatgccacac ttatattagt gtggatttcc tgttaatagc
     1561 aaacagcgtt cttgccagat gttctttccc gggttagctc ctcattatga ataggataat
     1621 gagaaggagc tgtagtttat gccgtgatga tacctggtat ggaagccggt tctcccggcg
     1681 gttaaacagg cccggttggt ttagccgtta ttcttgccta aaatagttta ccttcaccat
     1741 caacccccgc atccggtcag tcactaacta tctctaatcc cgggtacctg cggtcattta
     1801 tacacctaaa tttttaacat gggagaaacc tggaacaaaa aagaaagaca aaagaaaaag
     1861 caaaaagaga agatggacaa agcggagaaa atgaaagaac gcaaagaaca gacaggaaag
     1921 aaaagtctgg aagacatgat ggcgtatgtg gatgaaaatg gtaacatttc cgccactcct
     1981 cccgatcccc gtaaaagaaa agaaatcaaa ctggaagata ttacgatcgg cgtaccggaa
     2041 tatgtacccg atgctgagga tgaattccgt accggcaaag tcagcttttt caatcatgaa
     2101 aaaggatttg gctttatccg tgacctggta acacaggaaa gcatatttgt acatatcaat
     2161 aacctctcag gccccgttca ggagaatgat aaggttagtt ttaccaccga gaaaggtccc
     2221 cgtggtctga tggctgtaaa tgtgaaactg gtgccctgaa ccgggcaggt ttaaacctca
     2281 ctgagccggg taaaggaaat cccgttaaag atccggctca gattcaggtc cttccgctgt
     2341 tcccattctt taacattatt cccgcttact accctccaca aatttttgga tttcagttct
     2401 tcgtaatctg attcctggat aaatatgccg gtaaccggct gacggtcctt aaaatgaata
     2461 ttaagcggat tgtcttttct tttgcccggg gcaatgtact tttcgatttg ttctgaagtc
     2521 ataactgatt atatatttta agcagcagct ctcttcaaac tgtcaggact gattaatcca
     2581 tccaggttta ctgctcttgt ttaaaattgt tgtctgggtt ttgccatatt cactaccatt
     2641 ggccggccct cacaagggga accattcagg tcattaatgg ctttctgcgc ttccagctca
     2701 tccggcattt caataaaggc gaatccacgg ctgcggttag taaacttgtc ggtcattatc
     2761 cgggctgaac tcacttcacc aaaagggaca aatactttct tcagctgatc atcagtcatc
     2821 gtgaaactga gattggaaac atagatcgtc atgggaatca ttttcgggtc aaaaaatagt
     2881 tgaggcgagt gcagctgtag ggaaaatgaa aagggaaagt gattcacgta caagagcaga
     2941 gtactcaatt tgtcccggtg aaggtaggtt aattttaggc ggtgccggat tttcttttca
     3001 aaggcattga catcggaacg ttttaatccc ggcccggcta aaaccggcat ccgaagccca
     3061 gttactcaca tttattctgt tatgtatgaa ttttttgtga ataagttgat cgccccgcaa
     3121 gtaatttttc gaataatttt aaaatatgca agaccattac agccttctcc atactatcta
     3181 cgaaatcgtg aagaatgatc cccagccgga gcgttattcc tgcaagcccc gggaactgat
     3241 tctgcgtcgt atgcaggaat ggtccgatat acagcaacaa ctgcatcagc ttgaaaaaga
     3301 ggaattagta acgaccgaac agcaggaaac catggtcatt cgtattacta aggccggtct
     3361 ttcgctggta aaagaacagc ttgaaattgc ccgtctcagt ggcaattgat ttattttaat
     3421 ttgctgctga tcttttctcc ttcaacattt acggaatcag gagattgttt tgtgcggttt
     3481 aaaaacagcg ccacatcatt cgtatgtgcc agcgtttctg gttttgtcat ggttgctaca
     3541 ctgccatgtc cttcaaaatg gtaatgcaca ctgataaact tcttcacatg tttgcgggtt
     3601 tcagctggta gccacttttc cagttcccag taattgcggc tgcctgattt ccggattgca
     3661 gacaatactt ttgccggtcc gctgttataa gcggccaggg tcagcaacca gtcgtcaaat
     3721 attccattca ggtaaagcag gcatttggcc gcggccaccg tgcttttata ttcattccgt
     3781 cggtcgtcct gcttgccgga aatacgaaga ccgaaacttt ttgcggctac tggcataaac
     3841 tgccataggc cggcagcacc cgctgaggat acgatccggc tgtccagttt cgattcaaca
     3901 atagccaggt atttcatttc taccggcacc tggtactttg taaatacctg atcaatcatg
     3961 gaaaaaacgg gaccgctttt cgcttttatt ttatcatagt aaaaggcatt ttcacgcaga
     4021 tgagcctgta caaatacacg tgcctcctcc tgtaaacgga tacggggcgc agcgtctaat
     4081 tctgcagcag ttaaatcggg caaacagatg cacaagcctg ccgtatcaaa catatgtttt
     4141 gaaataacca gggagtctgc cggtactgaa acagtattta atttctgttc agcaagggta
     4201 atttttttat taccggaagc ggcggccatg agcatgaaac tggtaatgat aaatccagct
     4261 ttgtgtaatt tagttctaag catgtaacag ggttacgtga ataatacttg ccggactgga
     4321 tcagaaaatt tttcttgcgc attgcaaaaa aaaacgggtg ttgcggacaa gagtagtttt
     4381 tcaataacac aacaccgggc ttattaatac caaagagggg caaaggtaac cccggaaaca
     4441 gccctatttg ccgcttttaa cagctcttta tccactgaca gtttaactcc gtgagaatca
     4501 ttgaacaaat ttgttaaaac tgcttatttg tcagatgtca gttgtcagtt gtcagatgcc
     4561 aggaaacagc ccatgctaag taggtgttcg ctgttagctg ttaggtaaca gcccttcctg
     4621 acaacatata cgtcaatgca cgtcagtcgt acttctaact tcttacttaa agaaaagggc
     4681 cgtatcttat gatacagccc tcgcttttaa aagagtttca aagattataa ctttgtcacg
     4741 actacttctt ctacgttacg gttgttgttg ttaactgaat agagtgcaga tttcttgttg
     4801 ctcttggtac tgatcacaaa ctggatgctg tagttagcac catcgttgtt ctcatccagt
     4861 gacaacatga atttcttggt gaaaccagct gcttttatgg tttcaatgtg aagcacattt
     4921 ccatgttcat cccgaacact cacaatgtac tcttcctgta caccagcacc tggcacagtc
     4981 agttcgaata caggattgtt ctgcactttg cctacgaatt tgatttcagc ggcaattaca
     5041 ggtttctttt cagtagcgtt aactacgggg ctaagggcga tggtgaacag ggtcagcagc
     5101 gcgatagcaa tggagcggtt gtttaaaatg atctttttca tggttatact ttttttgttt
     5161 ttttaaatga ttacgaaaaa ctttaatggg ggcaatcggg caggcaagca gaaagtttca
     5221 gggcaatgca gaggttagaa atccggatta gtttcgcaag agttacttgc tttgatgatg
     5281 taaaaatagg gcgggttttc ggtgccataa aaccaggttt aagctgtttt aaggataccc
     5341 aaggctatat ttaaattctt atataattga taaacaatag tttacggatg gttttataaa
     5401 ggatacccaa ggctttaaaa ggcatataat gagccgtttt ttcggtattg ttttaacaga
     5461 ttttaacaga ttatcaggta gactaagagt atcccctcat tatttttatt cctattatat
     5521 tagcgtaaaa agatggtctt catatactta ataaggtgtt gagaaattaa tgttgagcgg
     5581 atttggctag ggacaaaaaa acattacctt caaccgttcc ggagggctga ctgattggca
     5641 taatcgtctt tctttcaacc aaatggccgg aaatttgtta tataaatcag gtcagcacca
     5701 tataggatgg tgatgcacaa aaaggagttt taaaaatggc atttaaaaaa gatgatgtaa
     5761 ttattatcga acccagtgaa atttttgttg gtaaaaaaga cgcgcctgtc acattagtgg
     5821 agtttggcga atatgaaagt gaggaatgtg cgaaagtgaa tgaagtggta aaacaattgt
     5881 tggaagaata tgaaggcaag atccggtttc agttccggca tttcccgctt accctgattc
     5941 atcagcgtag cctgaaggcc agtgaatccg ctgtagccgc ggcccaggaa ggaaagtttt
     6001 gggagatgca taatgtattg ttcaacaacc gacgtaatct cggaaccacc agtcttaaat
     6061 tacacagcaa ggaagcaggc gtgaagaata aaaaattcct ggatgacctg gtgaatggta
     6121 tgtacggctg gcaggtgcag gatgatatta aagaaggaca taaccggggc gttaaagaat
     6181 tgcccacatt ctttattaat gatgtgctgt tcgttggcaa acctactttc gccaacctga
     6241 gtgccgctat tgattctgcc ctgaaaaaag gtaaatcaaa ggcaccggta aagaaagctg
     6301 ccgtcaaaca aaagcaaaga gcgtaattaa cgcctcagca tattgaatcc cgccggtggc
     6361 gggatttttt ttgcctgcgg caaaaggggt ggccccaaaa cacacaaaag aacacgaata
     6421 aacagccggg aaacctgcct ggcggcagtt ttttagccac ggatatcaca gattcacacg
     6481 gattgatttc ttatccttat gcatgtgcct gttaatttgc atttaaaatg ggataactct
     6541 gaaatatgga aaagtaaatc cgtgttaatc agtgttatcc gtggcttgta ttcctgtatt
     6601 tttcgtgttc tttcgtgtcc attcgtggcc accccttttg ccgcaggcaa aaaaaagctg
     6661 cctctttaag agacagcttt tacaaactta cggtatatga ttaccagcgg ttgttgttgt
     6721 taccagagta agaaggacgg ctgttgttag aacgggcggg tctttcttca cggggtttag
     6781 ctacactaac tttgatagaa cgaccgtcaa cggttgcgcc atccagttca gcgattgctt
     6841 tgttagcagc agcatcatcg ctcatttcca caaaaccgaa acctttgcta cggttagtga
     6901 atttgtccat aataaccttt gcagaagtaa cgtcaccgta ctcagcaaaa taacttctta
     6961 agtcttcatc cacaacggcg aagcttaaat ttgaaacata aatgttcatg taaaaaaaat
     7021 tgacttttaa aaatacttga gaaaacgcaa gggtaaagaa actaactatt agcagataat
     7081 gcgtagcagt aaaaatcact tacaaactgt cgtcgaactc aaattaacag ggcaaagata
     7141 ggagtaaaag gaatatccaa gggatttatt ttgttaaagc gggttttgtc aggtttaaga
     7201 ggtttaaaag gttgaagagg tttaagaggt ttaaaaggtt gaaaaggttg aaagggctta
     7261 gacgtttgtt gtttagtggt ttaactgagt aaactctttt tatgactgct ggttggtcaa
     7321 acagaactgc cgcaccccat aaaaccagcc ctccttagaa cagactacta actactatct
     7381 gctaactact aactactatc tggctactgt ggttctacat taaacatatt aaccccctct
     7441 tctttcttcc gcatcccgaa actataccgg atattaatcc caacccgacg ggtatcggcc
     7501 tggcggaaac caatagcatc cactgtacct tgtttcagac tgaaatcatt tttattggtc
     7561 aggaacatat cgcttacgct cagggttatg atcagctttt ccttcatgaa ccgccggtta
     7621 atactggcat ttaatgctcc aaatgaaccc agctcataaa attgttgctg tccttttaac
     7681 cgccagaatc cattcagggt aataaccgat agtttatcca gcttcagggt ttgataggta
     7741 aagaaagtcc aggttcctct tttaaaagag agcggagcgt tctcataaat tccttcatag
     7801 aaattgtgat tgtactgagt accgagtaca aaaaagtatt ttcctccggg tggcaaagca
     7861 cccagtgccc tgaagtacca ttccttgttt ttcccgagat tgtcataggt ccggtatgcc
     7921 tggctttgac tcgtatcgga ctgatacacc acattcgaga aaatatcttt ggtgtcgttg
     7981 accccaatgg cgagaatcgg ccgctcgtcc acactgatgt tggcttcata attccgggta
     8041 aactgtggcc tcaatcccgg attacctact tcagacagat aggcatccac ataacgggag
     8101 aatggattca gttgttcata caccggacgg gtaatagtac ggcgatatac gaggtaagcc
     8161 cggagatcat agcctgctat tttcatcagg cttttactca ggaatacata gggaaacaga
     8221 tccgtacgat gaatggaaaa actggtatcg gtggggattt cctgattccc ccgcatattg
     8281 gtgttttcca gccgggtccc aaactttagt acaatatctt ttcccatggt ttttgaaccc
     8341 tgggcataag cagaattgat attctctttg taacggaacc ggttggtacg acccgcgtcc
     8401 ggcacccggt tgccattcga ttcagtataa aaaagggtat tgttgttaaa caaatgaagg
     8461 gttgatttca acccggtttc taacgtaagc cggtttttca ttttcagttt gagatcagta
     8521 cgccccgata acaaatcccg gtgattgtcc atggtgccat ctccagaatt agtaaaaggg
     8581 atgggggtat aataccgggt gtcatacccc tggttatttt tattattgct gttataataa
     8641 aaaaactggc tggtccattc ggaacccaaa ctgtccagtt tcagttttga ctcaaatccg
     8701 tttccgatta ccagcgaatt accctggttg gttacctggt tcagattatt ggtcagtaac
     8761 tgattagtgc tgatcttttc aatccggttc tggttatcgg ttttattgtc cgacaaattc
     8821 agattagccg ttccggataa ttcaaactcc cattttttct ttccccagct ataaacataa
     8881 ctgccaaaaa aagtattgcc cggatatgtg gtataggctt cctggccgag tacagaatcc
     8941 ggggcaaaaa tccggtctgt gacaattttc tcataactat tacgatgact gtaattaaag
     9001 ttcagcgata aacttttttc cccatcatta ttattcaggt taaatccaat gagctgattt
     9061 ccgtaattgc cctgctggat accggcattc acactgcccg taagtccgat ctttacccct
     9121 tttttcaaca ccacatttac cacaccaccg ctgctgttgg catcatattt tgcagaagga
     9181 gttcgtacaa tttcaatttt tgaaatagcg gtagggggca ggctcttgag tatactggcc
     9241 acatcagcgg cgctcattct catttcacgt ccgttaattt gtacactggc cggactcaga
     9301 ctactgatat aaatattccc gtcctggtcc acaaacaagc ccggtgtttt ttcaatcact
     9361 tcatatccac tggaagaggc cgccaccagg ttttcaggat ccacaatagt tttgtcatct
     9421 tcctggcgca tcagcggttt agacgaccgg agtacaaccc cttccatggt ttttcctgtt
     9481 tcttccatag taaaacgaaa atcaggtgta cccgaaccca ccgaaatatt tttttcaaag
     9541 ggggcataat tcagcgccac tacttcaatc cggtactgct gtcccttaat gagtggaaac
     9601 cggaccagtc cattgctgtc cgctacctgc tgtttagcca gggtactgtc catgcggtta
     9661 atggccgata cggaggcaaa tggaacaggt tcttttttac tgttctgtaa ccggatggtt
     9721 acaggtacat cctgcgcata caggataccg gtggaggaaa atagaaagca gaggataagg
     9781 ataaggctgg tatgttgttt catggtgctc attaacagga aagcgcaaaa atagttcttt
     9841 ctttttcctg caaaagccac agatcatgcc ggatagcctt ttcccatttt tttaacgctt
     9901 tgattcatct tgtgtcctga ttttatcctg gtgagtattc tccgcctcat caatggaatg
     9961 ggttcggtga atattcttct ccagtttggc cagttcggac agtttaatat agaatttctt
    10021 taatgtctcc agttcttctg ctgacaaatc ttcaacatct accagccggt tgctggccct
    10081 ggaagaagcg gcaataagtt cattcagttt caactgcagg gccacactgt ctttattctg
    10141 cgcatgctgg attacaaaaa ccatcaggaa agtgatgatg gtggtaccgg tattgatgac
    10201 cagttgccag gtatccgaga atttaaagac cggacccgtg aaaatccaga tcaggattac
    10261 caggcaggcc atcaggaagg caaccggccg gcctgttgcc ttggttactt tcacggcaaa
    10321 tcgttcaaaa aaggggagcg tagatgattt cataccattt tatttcagcg ttttactatt
    10381 ttagttacca tattaaaatt agtacttaat cattacgatg gtccggttta ccgttacgat
    10441 aaaaaaattc gggtcaaaag gggagaagac cggctggtct tattttgaag tgcccgctga
    10501 tattgcacag gaacttaaac ctggcaacaa aaaggagttt aaggtaaaag ggaaactgga
    10561 taaatttacc attaagcgaa cctccctgat cccaatgggt ggaggacttt tcattatggc
    10621 cctgaatgcg gatatgcgga aagccattcg caagaaagaa ggggcaatgt tggtggtaca
    10681 acttactgaa gataaatctg attttgtctt taatgctgat tttatcgaat gcctgaaaga
    10741 cgaaccacct gcacattcat tttttcaatc acttaccgga tcacaccaac gctatttcag
    10801 caaatggatc gatagcgcta aaacggatgc cacgaagacc aaacggattg cgatggcggt
    10861 gaatgcgctt gcccggagtt tgggatatcc tgagatgatc agaagcagta ggcagtaggc
    10921 agttggcagt aggcagtcgg cagtaggcag taggcagttg gcagtaggca gtcggcagtt
    10981 aacagcccct acttagtatt ttccccgata tactttcact ttttgttact tttttgcaga
    11041 tccggattga ggaattttta ctttttactt ttgaattttg acttcactta aaagggttcg
    11101 tatacagctg attcgtctgc actgtccggt cggtcagcgt cttcagaaaa gccaccaagg
    11161 ccgctttatc cggtgcagac aaattcaaaa aacggggctg cccgttaggt agtttcatct
    11221 gtggcgaaag attgggatga ttctttacac cggtgctgta atgctccacc acttcttcca
    11281 gggttttaaa acgaccgtca tgcatatagg gcgcggtgag ttccacattg cggagtgtgg
    11341 ttactttgaa cgtggcatcc tggttagtgt tattggagac ggcgccaaaa ccacggtcca
    11401 ccgtagtcat atcaatacca ttattctttt ctgtcggggc tacaaaagtt tcggtaccat
    11461 gacaggcggc acagccattg gcgggagtca gaaacaattc cttgcccctg ttttcctgcg
    11521 ccgtgaagtt cgggaagacc gcgtttgggg ccggggccgg attggcagga aggctggcac
    11581 ggcctgcatc atatttagat tgataggaaa caatggaccg gacaaattgt gacagcgctt
    11641 tggaaataag gtcactgttg atggtttcgt taccaaaagc ttttttaaat aaagggggat
    11701 aataggcaag ggcattgagt ttgttttcca atgccggaag ggtcattccc atttccacca
    11761 ggtcttgaat gggggctact acctgagctt ccagggtggc cgcccgttga tcccagaaaa
    11821 aactgcccgg ggtataaaac cgggcattga ttaatgacat agagttgcga ccggttaagc
    11881 caccattgaa acctttgctt ttaaaggcgg tatcggaaaa ccccaattcg ggtttatggc
    11941 aggaggcaca ggaaatcgta ttgttggcgg ataaattttt gtcgtaaaaa agtacccgtc
    12001 ctaatgtggc tccgtcatta gtgatcgggt tagtggccgg ggtattgatt tgtccctgga
    12061 taggcgggga attcaggtag cccggcaggg aaatgctggc atagttgaac gggttggctg
    12121 gcagattcaa tacagccgaa acgggatcat tcgccggggt gctggtctct tttttacagg
    12181 caaggaaaat aaacagggca aaaagtccga tgatggctag cgtctttggt cggtacatga
    12241 tggttcgatt ggtggtcggg ctaagactgt ttaaccaaca atgggtttaa catccgcgaa
    12301 aaaataagtg tattgacaat tgtctgggta aacttacgtt gtcccattgc ggaaaggggt
    12361 aagcgaacct acaggtaaag gaaattaatc agaaagtttt gtaaaaatct ttcttcaatg
    12421 actatttgct ttttgtgatc acccggatca gcgaatcttc ctcagcggtt ttcatgatgg
    12481 tgacggtatc caccaggctg ttgggcactg ttgtggacaa cacatattgc tttagtttcc
    12541 attctttgtt ttgtttaact atcacacccg aaccgcggca gatcttcatc tgggtgctga
    12601 gtagttcatc aaaccaggcc aggtctccgg caggatcaag ataaatatgc cggtcaatag
    12661 ccgtgaaatc ccaggccttg cccttatcga aataaggttt ggaccattcc ataaactgct
    12721 ttttgtccca gcgctcggtg gcatccgtac cacagaagat accgtcttcc gtataataat
    12781 taaaataggc attgaaatcc gcacgggccg ctgcgcggtt aaaggaatcc agcgtattac
    12841 cgatttcttt gatggcagtt gccggatcgg ttgcagtgtt gggttgctgg caggcaaaga
    12901 gaagaatagc cagtgccaag gggagaatag tttttttcat gtcttgaaaa taagaaaata
    12961 gaggagtatt cccatgggtg tttactgaat accatcccat tttcctgtga tttacagggg
    13021 gatcaccaca aagcttattt agcttttttc atctccacca cccagatacc acgcaattca
    13081 ttcagtttaa aaccggtggc tttatccccc gggtatagcc ggttgagggt ggagagcgtg
    13141 gctggcttta tatctgtttt tttatcccca tgacattgaa gacatagttg atctgtcagg
    13201 ataggataat aagcagtgta cgtttcagtg gtttctttca gctgaggttt aggattctct
    13261 ttcctggtga gtgaagcctt gatttccttt atataggaaa gttcatctgc ggatgccacg
    13321 tttaccggat tccggtttct ttcggccacc cttttaatgc gactgcccaa caccgtactc
    13381 atactgtctg tgagctgaat agcccgggaa gaacaaaatt caatggcgcc ttccgtaccc
    13441 atatcctgta tggctgtaac caggtgcttt cccaaaatgg ccttggtttt catcgccatt
    13501 tcctgtccgg tttccaacgg actttttatt tgactgattg tgtcggttga agcggtgggg
    13561 attccctttt tcttctcccc tgttgtattg cagttggtca gaaagatgac cactgccaaa
    13621 atgctgagta gcaggatagt ttttttcatg tcgccggtat ttatgggtaa tggctaaagt
    13681 tcgtttactg aacggtaaag ttcagtgctt ttggtcaccc agcctccctt aatcccagta
    13741 agtgccatcc agtcccaaaa ctgttcctat aaacagtccg attaaaaaga cgatcaggca
    13801 aaggaaataa gctgtggctt ttaaccaacc ggctttactt cgccagggtt ccataaaagt
    13861 aaagaatagg ccacccatag ctccggcaaa gggaacgatt acgaatgggc ggatcatcca
    13921 tagttcatgc cattccgggt tgggttcatc cgctttaaaa agaaaaagcc caataagggc
    13981 taaaccgatc ccggcgccca gcagcatgtt tttagccagc gatggggact ggaatgtttg
    14041 tttcaggtac ctgaaaagga tcatgtgatt agcttgtgag ataaaaagta ctttgaaata
    14101 caaagtaaat gtttaatttt gactttgaca agattttttg atctgaaatg ttagctatgg
    14161 caggttttgt aaaagaagat tctattgtac ggcgtatctg gggccacagt gatacagtcc
    14221 tattcatttt tgccggggca tcagctgagt tcgctttaaa taaggcggta gactggcttt
    14281 actttaccgg taagctgcct gccgatccgt tgggaagatt attctccacg gtaggttatg
    14341 ccaaaaagat cgtctttgcc ccggaagccg aagcccaaca ggcgatcggc cggatgcgcc
    14401 agatccatgg tgcagtggag cagaatcgcg gcagtgctat tccggaatgg gcttaccggg
    14461 atgtgctgta tatgctgatc ttttattcca tcgcctctta tgaactactg gaacgaaaat
    14521 tatcggagga tgaaaaggaa gaagtatata acgtctttta ccgtctgggt tcaggaatgg
    14581 ggttgaaagg actgcccacg acgtatacca cctggctgcc cgaccgggaa gcgcatctga
    14641 gatcggatct acaaaggagc caatacaccg cggatctttt ccggcagtat aaaaagcacc
    14701 tgggcacatt ccgttataaa ctgctgatcg aagcacagaa aatgttattg ccagacactg
    14761 taaaagaaat gctccagttc aaaactatcc gatggttgta cccggctgtg ccagtttaca
    14821 aattcagccg gatgatgcgg ttggatagta tcattaaaaa tgtattactt ccggggaaat
    14881 ataagcagga gatcaggggg ctcgatgtgg ggtgagtggt gagtggagag ttttccccat
    14941 ccccctcatt ttccgtttct ttgcagcatg atatcctcca tcaacgactt caaaaatccc
    15001 ttctttatcg gtgtggccgg ctcaggcatg agtgccctgg cccagtacct caaaggcatt
    15061 ggcaaaaacg taaacgggag tgaccgtttt tttgcccccg gtgtatataa tgaaacccag
    15121 gaaaaactgg aagccgaagg aatccggtgt tttgtccagg acgggtcagg gattaccgat
    15181 gcgaccgacc tggtggtttg ttccaccgcc atcgaggata cggtaatgga agtccagaaa
    15241 gccaaacaac tcaatattcc tatactccga cggtccgaac tgctttccct gattgcgggt
    15301 agcaagaaaa cgatcgccgt tggcggtacc agtggaaaga gtaccaccag tgctatgttg
    15361 tttgacatcc tggagaaagc cggactggag cccagtatta tcagcggggc aggactgatc
    15421 agtatcatca aagagggtaa gatagggaat gccaaggttg gagcggggga actcctcgtg
    15481 attgaagccg atgagagtga tggttcgatt gttcagtata agcccgaaat cgggctgctg
    15541 atgaatattg ataaggatca ccaggagatt gacgaattgc tcagtctgtt cgggaccttt
    15601 aaaaacaatt cccgcaaatt tatcaccaac cagtcgcatc ctttgacggc tccgctttct
    15661 tcacagtggg ccaatgattt tgccacggat accagtcatg atgcaggata tgccgccagc
    15721 gatttcaaac aagtggggat ggaaatcagt ttcaaagtaa ataaccagtc tttcagtctg
    15781 caccttcccg gcaaacacaa tatggaaaat gccgtagctg cgactgcagt tgctcaccag
    15841 ttgggcgtgg atctggccac ctgtgcggca gcgctgaaag agtaccaggg gatctaccgc
    15901 cgtcaccagg tattgggaca aaagaacggg gtatggatga tcgacgacta tgcccataat
    15961 cctgtcaaat gtgccgcagc cattgctgcc tgccagccga tcgcccccaa agtagtcgcc
    16021 tggtttcagc cccatggcta taaacctacc aaattcctcc gggctgattt tgtaaaagaa
    16081 ctggccgccg tcctccgtcc ccaagatgag atatggatga gtgaaatctt ctatgcaggc
    16141 ggtaccgctg taaaagatat atccgccaac gacctgatca atgatctgaa agcattgggt
    16201 aaaaaagcgt tttttgtaga agaccggaat gacttcatag acaccgcccg ccctcacctg
    16261 gatagcaata cggtattatt gctcatggga gcaagggatc cttctctgga gcaatttgca
    16321 aaagagagtt gggagagg
//
LOCUS       DAOPMW010000035        16255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3506_length_16255_cov_7.292901, whole genome shotgun sequence.
ACCESSION   DAOPMW010000035 DAOPMW010000000
VERSION     DAOPMW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3506_length_16255_cov_7.292901"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..692)
                     /locus_tag="PLU11_03310"
     CDS             complement(174..692)
                     /locus_tag="PLU11_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58169.1"
                     /translation="MKLDYQYLLEGNLDPQSRVWIYQCNRLFSLSEAFEVEDILKQFT
                     GSWLSHGTPVKGAAYLFFGQFVVLMADETASGVSGCSTDTSVRLIKDIEQRFGVTLFD
                     RLSLAFVVKDKVELLPLSQLPYAAENGFINGDTLYFNNLVQTKKELETNWIIPVKESW
                     LAKKIEFSGPVS"
     gene            722..1129
                     /locus_tag="PLU11_03315"
     CDS             722..1129
                     /locus_tag="PLU11_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58170.1"
                     /translation="MTMHGKNDSWQTNWLYDSAEWKRFAKWELFKKGGISLLLCLLIP
                     FRYKKSKEVKVSQHQVLIDGKTYLFNAGKRVFQTADLYDAGPLHVLEICFKENESYGV
                     IRVPVPKGKLKEAIVLEKRLQVTNVSLIVTREV"
     gene            complement(1448..3844)
                     /locus_tag="PLU11_03320"
     CDS             complement(1448..3844)
                     /locus_tag="PLU11_03320"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58171.1"
                     /translation="MKRFPLLLLVFLCSIAGSAQDKTVSGILTGNVMDSRSKALEGAT
                     VRLISFADTLTNRTMLTDKAGEFSFSSIPFGYYKLSVSFVGLQPLTLDSIYFRSERFD
                     FNMADITLKPKSNEMLEDVVIYAEKPLIQSKDGNITFNAGESALAAGGTVSELLTQVP
                     LVTKDQDGKISVRGKEPKILIDDKPVTLNMQQLQDLLESMPGSSIEKIEVMTNPPPQY
                     ANEQGGVINIVTRKGRVGKSGRLSLSGGTRGEFLFNGSFNYRKQGLSISMNAGAGYNK
                     YGGSGYSIRNNIYTDSSNFFNTRNTNWSESIRPNFRFNMDYDFNKKHSVNVTLNYNQS
                     DAESYNNTEYKNINRFNELWKLSEREIQSLGKGYSPNASFTYTYRGKPGEVLRIISSY
                     HFSWNESNRDFNQQFYNPDYTPTGLDSMQRQNNNTRVNGQTLNLNYDRMLDNKKTFIS
                     VGGIYNRSNNHVTIDASYKKKPEGTMEKLDLLSNNFWFHQTVGNLRASVRQLLGKDFS
                     FTAGSTAEHTGIWFELLKENREVRNNYLTWLPFANINKTWREILSLTFAYRRSIRRPG
                     IGELNPTIDFSDPYNVRFGNSELEASTSDNFDFIIGRTKTKYFINLGFGFNKVENIFS
                     QVRTLLPDGKTQITWENISDRKELEVSTWNGVTLSKKLRVNFSSSYTYNMYSAFDKTV
                     RRFRDGGSFTSNLNFIYNPTDKWSFNGGFNINRFASAQGFARWNTSMNMGLMRRLFNK
                     RMTITLSTVDPFVNQQRRTFTYGTNFNLESYSLTRTKNYRFTIGYNFSNAPKKKSQKT
                     "
     gene            4010..5452
                     /gene="miaB"
                     /locus_tag="PLU11_03325"
     CDS             4010..5452
                     /gene="miaB"
                     /locus_tag="PLU11_03325"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPN58172.1"
                     /translation="MIDLMTDKVPRSDRDNQDEGRQGEAYAPFVQDPNQYKKRFYIES
                     YGCQMNFADSEVVAAILNQEGFGATRNLEEADLIFINTCSIREKAEQTVRKRLTEFRK
                     LKQRKKGMLVGVLGCMAERLKSKFIEEERLVDIVVGPDAYRSLPELVAEASGGQKAVN
                     VLLSRDETYADISPVRLDTNGVSAFVSIMRGCNNMCSFCVVPFTRGRERSRDAASILQ
                     ECRELFENGYREVTLLGQNVDSYYFVEENSNRIITFANLMEEVARLSPLLRVRFSTSH
                     PKDITDEVLHVMARYENICKYIHLPVQSGSTRVLQLMNRTYTREWYMAKVNRIREILP
                     DCGISSDIISGFCTEEEKDHRETLDVMEFSRYDMSYMFFYSERPGTLAQRRYTDDIPL
                     DVKKRRLQEIVDLQGRLSFESNKRDLNKTFTVLIEGDSKKSNQDWMGRSSQNKVIVFP
                     KEQYSLQKGDYVKVQVTDCTQATLLGKIIA"
     gene            5480..6733
                     /locus_tag="PLU11_03330"
     CDS             5480..6733
                     /locus_tag="PLU11_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN58173.1"
                     /translation="MDIQSIKNRFAIIGNSPALNYALQVAIQVAATDLTVLINGESGV
                     GKEVFSQIIHSLSSRKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGAVDARKGYFET
                     VNGGTIFLDEIGELPLGTQARLLRVLETGEFIRVGSSKVQKTDVRVVAATNRDLLQQV
                     QAGKFREDLYYRLSTVPIRVPALHDRPEDIHLLFRKFAADFATKYKTTSVQLDDEAKQ
                     LLISYPWPGNVRELKNIAEQISVLSEDKQIRAKDLGKFLQPYSSNRLPVLSSASSAAN
                     GPEFANEREILYKLFFDMKKDVTELKRMFLDMLQNPELVAQNPHILNELKDLRHSGQE
                     MLQPMSFPGGLQSPAPHTQPVLMNNDIQDHVEVEESLNIMDKEKELIIKALKKHKGKR
                     KDAALDLGISERTLYRKLKEYDIEE"
     gene            6733..7257
                     /locus_tag="PLU11_03335"
     CDS             6733..7257
                     /locus_tag="PLU11_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPN58174.1"
                     /translation="MKKLSSLIPAGLLALVVAAALFNSSSCGIYRFSDAAIPDSIKTI
                     KVNLLENRASYVNPSLSPNLSDKLRQKILSQTKLSQTNNDNADWEISGTITQYSFSTS
                     AITGQQASNNRLTVSISIILQDRKADKTEKHEVSRSFEFRGDQSFQQAETNLAEEMIR
                     TLTDDIFTKLFSKW"
     gene            7313..8254
                     /locus_tag="PLU11_03340"
     CDS             7313..8254
                     /locus_tag="PLU11_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58175.1"
                     /translation="MNVSINQLVKSLLQKESLEHCSLPELTAYAERNPYFGAAQLLLA
                     KKLQTESPERFEEQYQKTLLYFHNPLWVEHLLNDTGGAEIEEVEKVERLTSLQVDKVE
                     EVQEVEEVQEVEKVQEVQEVEEGAERLTSLQVDRVEEEVEKEIILDEEKMETGDNMNS
                     FETAVVEEVRADAPVIPVPVPVEAVNPANDLVFEPFHTVDYFASQGIKFKEEERPTDE
                     FGKQLKSFTDWLKTLKRLPATELAKNITPQSENKVEQLAGQSITDREILTEAMAEVWE
                     KQGNALKAMEIYRKLSLFEPAKSAYFAAKIEGLKKKN"
     gene            8256..8612
                     /gene="secG"
                     /locus_tag="PLU11_03345"
     CDS             8256..8612
                     /gene="secG"
                     /locus_tag="PLU11_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPN58176.1"
                     /translation="MTTLFVILIIVVSAVLGLIVLVQNPKGGGLAGNVAGFSNQLMGV
                     KQTTDVLEKGTWIFGGLIALLCLLSVFFISNTTTANASGPGVDPTKVTVPVQQQPQPG
                     TNLQPTPTPAPVPPAK"
     gene            8688..9725
                     /gene="mltG"
                     /locus_tag="PLU11_03350"
     CDS             8688..9725
                     /gene="mltG"
                     /locus_tag="PLU11_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPN58177.1"
                     /translation="MKKKIILGTLVVLLLLAGFIGYKVAGPAVSDKNGKYLYIRSGDG
                     MKELLEKLDTGKVISSKAWFSRVAGWLNFKSVKPGRYELKNGMSLYAFVRKLRSGDQS
                     LVKMVIVKERTREAFASKMGRKFDTECDSLTMIRFINSNDSLQSFGLDTSTFLSVMLP
                     YTYEIRWNSTPARIIEQFQVAYKKFWTPERTAKAAQLNLTPLQATILASIVEEETTRK
                     ADKLNIASTYLNRLKINMKLQACPTAKYVSRNFQLDRITEKQTSLVSPYNTYLNEGLP
                     PGPICTPSLESMEAVLNAPATAYLYFVASYAFDGSTIFTSNYDDHMKYARMFQAEQRR
                     RADSVKKARSK"
     gene            9727..10695
                     /locus_tag="PLU11_03355"
     CDS             9727..10695
                     /locus_tag="PLU11_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPN58178.1"
                     /translation="MPLLDKILHRIETLPLVSFLIKKSKTTSLPGFNGIAIYDVIRFF
                     FGQVKTIGMTERASAVAFNFVMAIPPAIIFLFTLIPFIPITDEFQQEMFSLIRDVIPG
                     EKDNAVVIHFLEDFINNPRNGLLSLGFVLSLFFSSNAMMGIMRSFDKNYIGFKKRNGL
                     QTRLVAIKITLILYVFVFVSVLLLVARVAVLKWLGITDETVLAVINNLRLLVVVLLFF
                     ASISIIYRYAPSVHKKWKLINPGSILASFLMLLMTFAFSWWVTNFSNYNQLYGSISTI
                     LIIMALIFINSLVLLIGFELNVSISSLRKMADERKDNGTTEEGISA"
     gene            10728..11330
                     /locus_tag="PLU11_03360"
     CDS             10728..11330
                     /locus_tag="PLU11_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPN58179.1"
                     /translation="MKKILYAAFPAILLISGWLNTADPLPIGAALPQPEVKMKDISGQ
                     EVSFSDARKKNGLLVMFSCNTCPYVIKNQSRTKEACAFASANEIGVVLLNSNEAQRDG
                     DDSFEEMKAYAAEQGYNWYYLVDKNSVMADAFGANRTPESFLFDKDGKLVYHGAIDDN
                     AADASAVSRKHLVVAMTEMKSDKDVSVKKSRSVGCGIKRQ"
     gene            11455..11652
                     /locus_tag="PLU11_03365"
     CDS             11455..11652
                     /locus_tag="PLU11_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58180.1"
                     /translation="MLSRHQLNRIIVIGFMVLVGYALAKAFYHNSILGILLGLTSLGA
                     GVYFLYLMAKAKEEMEAEENA"
     gene            11693..12523
                     /locus_tag="PLU11_03370"
     CDS             11693..12523
                     /locus_tag="PLU11_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58181.1"
                     /translation="MKILFPWLLMLFISCQQKNKAGTSLLPALNAAGPMGSVADSPTV
                     RNSIHVFTALCDNKYQGIVPVPAIMGNGQDPANNLYWGWGYGIRTYFNKSKEWKLVTV
                     STPGYPVHERLVYKSTIGDYYLVADAYDGRAMKECLQDFLKALSGNHKKTIIAGNQQV
                     GIFGHARLLAFVGHNGLMDLTLPETFIQADSLKREGIVLACYSKSYFSSRFKDAGAYP
                     LLWTSNLFGPEAYSLHDAISGYIRGENAVAIQTRAATVYAKFTKCSVHAAKRLLVTGW
                     "
     gene            complement(12503..12976)
                     /locus_tag="PLU11_03375"
     CDS             complement(12503..12976)
                     /locus_tag="PLU11_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN58182.1"
                     /translation="MFFMPGIRLDAQEVRRVKITDMEKLMSESKHPLVINFWATWCTP
                     CVEELPSLIREVKKHEADSLQLILVSLDDKENFPEKLRDFIRARNYEGTFLWLDETNA
                     DYFCPLIDSSWSGAIPATLFLHKKNGYRKFVEAKLSDRTLQDILKEMFRSPSRNK"
     gene            complement(13058..13660)
                     /locus_tag="PLU11_03380"
     CDS             complement(13058..13660)
                     /locus_tag="PLU11_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPN58183.1"
                     /translation="MTPERSHKLLQVLSKRQPDLTVVLENVFDPHNISAVMRTCDAVG
                     IQEIYILNNRIPPHKKWGARSSSSAAKWLTIYQFEDAAACFSALRKKYKKILTTHLSS
                     DAVSLHDIDMTEPIALVFGNEHSGVSDEIRAMADGNFIIPQVGIIRSLNISVACAVTL
                     YEAFRQKTLAGHYERPGFEHEMKQGLLREWELREEGEVGS"
     gene            13737..15194
                     /locus_tag="PLU11_03385"
     CDS             13737..15194
                     /locus_tag="PLU11_03385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPN58184.1"
                     /translation="MKYPPVQNFINGAFITPQTQRHLPVISPVNGQVLSEVPLSGAID
                     LDKAVTAASAAFRGWSRTPIKERVQVFFRYKTLLEKHLDELAALCSEENGKTHAESVA
                     EIEKCIELTEFATSLPQLVTGEILEVSKGVECRTEHVPLGVVASVVPFNFPAMVPNWT
                     IPNALALGNCMIIKPSEKVPLTLGRIALLLKEAGLPDGVFNIVNGDSEIVTAICDHPG
                     IEAVSFVGSTRVAKLVYQRGTSHLKRVLALGGAKNHLIVLPDANTDMTAQNVVASMSG
                     CAGQRCMAAGAMLAVGNVEAIIEKICIEARKVIPGKNLGAVISQDAKTRIEQYITEAE
                     KQGAKILVDGRGVTVPGNEEGTYVGPTVIDYVRPEMAVAREEIFGPVISIIRTQTVDE
                     ALAIENASPYGNAAAVFTQHGGMARYIIERASAGMIGVNVGVPVPREPFSFGGWNESK
                     FGTGDITGKSSIAFWTRLKKSTVKWNPEAGINWMS"
     gene            15356..>16255
                     /locus_tag="PLU11_03390"
     CDS             15356..>16255
                     /locus_tag="PLU11_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPN58185.1"
                     /translation="MLNFYSASTRIVNTRRGITECLESALGSNTTDCDLVIIHASLGH
                     NFKELIEEAQLLAPHAKVVAASCCGIVGKEGVSETMKDIAIMAVRGKEFGLAWVNEIF
                     GNNSYEKAKEMAEELYRQQPDTRFIYFLASGIDIANDRCIEGIESVFGPEVTIFGATS
                     ADNMKGVVSYQAVAGQVFEHAAFMIGFADPTLEVNTQATHGFVAIGEPFVVTRAEGHY
                     IYELNGKPAWTEFTRHLGLPATATCGDSIPIGALAEKLSPELSKEYGNDHILRVVTRH
                     EGDTMLYATTCKTGTELWLTLRDE"
BASE COUNT         4421 a         3675 c         3820 g         4339 t
ORIGIN      
        1 aaccggctac ctacagcttg ggaagctgtc gctctaccag gtgagctact tccgctttta
       61 tgttccgtag ctttagcgaa ggaacatttt actccgtagc cctgcctgcc ggcaggcagg
      121 tttagcgaag gaacatttta tgttccgttt tttcagcaag gagcaataca aaattaagaa
      181 accggtcctg aaaattcaat tttcttcgcc agccagcttt cttttacagg tatgatccag
      241 ttagtttcca attctttttt tgtctgcacc aggttattga agtaaagggt atctccgttt
      301 ataaaaccgt tttctgctgc atatggcaac tgggaaagcg gcagtaattc caccttatct
      361 tttacaacaa atgccaggga taacctgtca aataaggtta caccaaaccg ttgttcaata
      421 tccttgatca gccggaccga tgtatctgta ctgcatccgc ttaccccgga agctgtttca
      481 tccgccatta atacaacaaa ctgtccgaaa aataaatagg ccgctccttt cacgggtgta
      541 ccatgactca accaacttcc ggtaaactgt ttcaggatat cttccacttc aaaagcttca
      601 cttagtgaaa aaagccggtt gcactggtaa atccaaaccc ttgattgagg atctaaatta
      661 ccctccagca ggtattgata gtcgagtttc attcctctaa attacgacca aattgatgag
      721 gatgacaatg cacgggaaaa atgatagttg gcagactaac tggctctatg acagcgctga
      781 atggaaacgg tttgcaaaat gggagctttt taagaaaggg ggtatatccc tgctactctg
      841 tttgctcatt ccttttcgtt ataaaaagag taaagaagtg aaagtcagtc agcaccaggt
      901 acttatcgac gggaaaacat atctgtttaa tgcgggtaag cgggtatttc aaacggcaga
      961 tctgtacgat gcgggtccat tgcatgtatt ggagatctgt tttaaagaaa atgaaagcta
     1021 tggagtgatc cgggtcccgg tgccaaaagg caaactgaaa gaagccattg tgctggagaa
     1081 aagattgcag gttaccaatg tatccttaat agtaacaaga gaggtttagt tgtttatatg
     1141 tttagtcgtt tgacgtttag aggcacttta ccttaatcac gccctctttt atcctccgaa
     1201 gctttagcga aggagtattt tatcctccga agctttagcg aaggagtatt ttatcctccg
     1261 tagctaagcg aaagaggatt ttctctcttt tttactcctt tgatctcttt agcgccgccc
     1321 gcagggcaag aaaggtttag ttgtttagtc gtttgacgtt tagaggaact ttacccgctt
     1381 taggattttt tactttttac ttttgacttt tgacttttaa cttgcccctg taaatcatct
     1441 tcccgcttca ggtcttctga cttttcttct tcggtgcgtt gctgaaatta tagccaatcg
     1501 taaaccggta attcttcgta cgggtcaggc tataactctc caggttgaaa ttggtaccat
     1561 acgtaaatgt gcggcgctgc tgattaacga acggatcgac cgtactcagc gtaatggtca
     1621 tacgtttatt gaataagcga cgcataagcc ccatattcat actggtattc cagcgggcaa
     1681 agccctgggc tgaggcaaaa cggttgatat taaagccccc gttaaaactc cacttatctg
     1741 ttggattata aataaaatta aggttagaag taaacgatcc gccatcccgg aatcgccgta
     1801 cggttttatc aaaagcgctg tacatattat aggtataact ggaactgaaa ttgacgcgaa
     1861 gctttttgga aagcgttacg ccgttccagg tactgacttc cagttccttc cggtcactga
     1921 tattttccca ggtaatctgg gttttcccat cgggtaataa agtgcgcacc tggctgaaaa
     1981 tattttccac tttgttgaag ccaaagccca gattaataaa gtacttggtc ttggttcttc
     2041 cgataataaa atcaaaatta tccgaggtag aggcttccag ttcagaatta ccaaaccgta
     2101 cattatacgg atcggagaaa tcaatcgtgg gattcagttc cccgataccc gggcgccgga
     2161 tactacgtcg gtaggcaaac gtgaggctga gtatttcccg ccatgtttta ttgatattgg
     2221 caaaaggcag ccaggtgaga tagttgtttc ttacctcccg gttctctttc aataactcaa
     2281 accagatacc cgtatgttcg gccgtactgc cggcggtaaa actgaaatcc tttccaagca
     2341 attgcctgac ggaggcccgc agattgccaa cagtctggtg aaaccagaaa ttattactga
     2401 gcaggtccag cttctccatc gttccttccg gtttcttttt ataactggca tcgatggtta
     2461 catgattatt ggaccggtta taaattcccc caaccgatat aaatgttttt ttattatcca
     2521 gcatccggtc ataattcaga ttcagggttt gcccgttcac ccgggtattg ttgttctggc
     2581 gctgcatgga gtccagcccg gtgggtgtat aatccggatt ataaaattgc tggttaaaat
     2641 cccggttgct ttcattccag gagaaatgat aactgctgat gatccgcagc acttcgcccg
     2701 gtttgccccg gtaggtatac gtaaaactgg cgttggggct ataccctttt cccaggctct
     2761 ggatctcgcg ttcactgagc ttccagagtt cattgaaccg gttaatgttt ttgtactcgg
     2821 tgttattata actctccgca tcgctctggt tataattcaa cgttacattc accgaatgtt
     2881 ttttattgaa atcataatcc atattgaacc ggaaattggg gcgaatactc tcactccagt
     2941 tggtattacg ggtattgaaa aaattggatg agtcggtata aatattattc cggatagaat
     3001 acccgctgcc cccgtattta ttataaccgg ctcccgcatt catactgata gagagtccct
     3061 gtttccggta attaaaactt ccattgaata aaaattctcc acgggttcct cccgataagg
     3121 ataagcgtcc gcttttgccc acccttcctt tacgggttac tatattgatc acgccgccct
     3181 gttcattcgc atattgtggt ggcggattgg tcatcacttc aattttttca atcgaactgc
     3241 ccggcatcga ttccaacagg tcctgcaatt gctgcatatt caaagtaacc ggcttatcat
     3301 cgatcaggat cttgggttct tttcccctta cggatatttt accatcctga tccttggtca
     3361 ccagcggtac ctgggtcagc agttcactga cggttcctcc ggctgccagg gccgactccc
     3421 ctgcattaaa tgtgatattc ccgtctttgg attgaatcag gggtttctcg gcatagatca
     3481 ccacatcctc cagcatttca ttcgattttg gcttcagggt gatatcggcc atattgaaat
     3541 caaaacgctc ggaacgaaaa tagatactgt cgagtgtgag cggctgtaaa ccgacaaaac
     3601 tgacggagag tttataataa ccaaatggga tactgctgaa actgaattcc ccggccttgt
     3661 cagtcagcat cgtgcggttg gtaagcgtgt cggcaaaaga tatcaggcga acggtcgccc
     3721 cttccagggc tttactgcgg ctgtccatta cattcccggt cagaatacca gaaaccgttt
     3781 tgtcctgggc tgaacctgca atactgcaaa gaaaaaccaa taaaaggaga ggaaaccttt
     3841 tcaacatgct agattagacg gtaaagttat aacgaacttg cccaacccgg acctgttttt
     3901 atacaaaagg caaatacaaa catctgttta tcagtatctc agggagttat gacaacctgt
     3961 cacattctcc aaggttttag gtatatttgc tgttctaaat cagggttgaa tgattgattt
     4021 aatgacagat aaagtgcccc ggagtgatcg ggataatcag gacgaaggac ggcaggggga
     4081 agcttatgcc ccgtttgtcc aggatccgaa tcagtataag aaaagattct atatcgaaag
     4141 ttatggttgt cagatgaatt tcgctgatag tgaagtcgtg gccgctattc tgaaccagga
     4201 aggttttggc gccacccgca acctggaaga ggctgacctg atatttatta atacctgttc
     4261 gatccgtgaa aaggcggaac agaccgtccg taaacgcctg actgaattcc ggaaactcaa
     4321 gcaacgcaaa aaaggaatgc tggtcggggt actgggatgc atggcagaac ggctgaaatc
     4381 aaaatttatt gaagaagagc gattagtgga tatcgtggtt gggccggatg cataccgttc
     4441 cttacccgaa ttggtggctg aagcatccgg tggacaaaaa gccgtcaatg tcttattaag
     4501 ccgggatgaa acctatgcgg atatttcacc cgtacgactt gacactaacg gggtgagtgc
     4561 ttttgtaagt atcatgcggg gttgtaacaa tatgtgttcc ttctgtgtgg ttccttttac
     4621 ccgtggacgg gaaagaagcc gggatgcggc cagtatcctg caggaatgcc gggagctgtt
     4681 tgaaaatggc taccgggaag tgacgctgct gggacagaat gtggacagtt attatttcgt
     4741 ggaagaaaac agcaaccgga tcatcacttt tgcgaacctg atggaagaag tggcccggct
     4801 ctctcccctg cttcgggtgc gcttttccac ttcacatccc aaggatataa ccgatgaagt
     4861 cctgcatgtc atggcccgtt acgaaaatat ctgtaagtat attcatctgc cggtgcaaag
     4921 cggctccacc cgggtgttgc aactgatgaa ccgcacgtat acccgcgagt ggtacatggc
     4981 caaagtaaac cgtatccggg aaatccttcc tgattgcggt atttcatccg atattatttc
     5041 cggtttctgt acggaagagg aaaaagacca tcgcgaaacc ctggatgtga tggaattcag
     5101 ccggtatgac atgagttata tgttttttta cagtgaacgt ccgggtaccc tggcccagcg
     5161 ccggtatacg gatgacattc cgttggatgt gaagaaaaga agactgcagg aaattgtaga
     5221 cctgcaggga cggctttctt ttgaaagcaa taagcgggac ctgaacaaaa cctttacagt
     5281 gctgattgaa ggtgacagta aaaaaagtaa ccaggactgg atgggccgga gttcgcagaa
     5341 caaagtgatt gtattcccga aagaacaata ttccctgcaa aagggtgact atgtaaaggt
     5401 gcaggtgact gattgcaccc aggccaccct gctgggcaaa ataattgctt aacatttacc
     5461 aggcctttgc gccaagacta tggatattca aagtattaaa aatcgttttg ccatcattgg
     5521 caactccccg gcgctgaatt atgcgttgca ggtagctata caggtagctg ctaccgacct
     5581 cacggtactg atcaatggag aaagtggtgt ggggaaggaa gtgttttctc aaatcattca
     5641 ttcattatca tcccggaagc ataatccgtt cattgccgta aactgcgggg caattcccga
     5701 aggtaccatc gattcggaat tattcggaca tgagaaaggc agttttacgg gagccgtgga
     5761 tgcccggaag ggatatttcg aaaccgtaaa tggtggaact attttcctgg atgaaatcgg
     5821 ggaactgcca ctcggtaccc aggcccgttt attacgggtt ttggaaaccg gtgaatttat
     5881 ccgcgtgggg tcttccaaag tgcagaaaac cgatgtacgg gtggttgccg ccactaaccg
     5941 ggatctgttg caacaggtac aggctggcaa attcagggaa gacctgtatt atcgcctgag
     6001 tacagtaccc attcgcgttc cggccctgca tgaccgtccg gaagatatac acctgctctt
     6061 ccgcaaattt gcagcagact ttgccaccaa gtataaaacc acttccgtac agctggatga
     6121 tgaagcaaaa caactgttaa taagctaccc ctggcctggt aatgtgcggg aattaaagaa
     6181 tattgcagaa cagatttccg tattaagcga ggacaaacag atcagggcaa aagatctcgg
     6241 taaattctta cagccctatt ccagcaaccg gttgccggtc ttatcatcgg cttcctccgc
     6301 ggcgaatggc ccggaatttg ccaatgaaag ggagattctg tacaagcttt tctttgatat
     6361 gaaaaaagat gtgacggagt tgaagcggat gttcctggat atgttacaga atccggaact
     6421 ggtagcacag aacccgcata tcctgaatga actgaaagac cttcgccatt ccggccagga
     6481 aatgttgcag ccaatgagtt tccccggtgg tttacaatct cccgctcccc atacacaacc
     6541 tgtattgatg aacaacgata tccaggatca tgtggaagtg gaagagagcc tgaacataat
     6601 ggataaggaa aaagaactga tcataaaagc gctgaaaaaa cacaagggca aacggaaaga
     6661 tgcggcgctt gaccttggaa tcagtgaacg aaccctgtac cggaagttaa aagaatacga
     6721 tatcgaagaa taatgaaaaa attatcatcc ctaatcccgg ccggattact ggccctggtt
     6781 gtagctgctg cgcttttcaa cagctccagt tgtggtattt atcgcttttc cgacgccgcg
     6841 ataccggatt cgatcaagac catcaaagtg aacctgctgg aaaacagggc ttcctatgtc
     6901 aacccttccc tgagtcctaa tttatcggat aaactgcgcc agaaaatact atcgcagacc
     6961 aagctttcgc aaaccaataa tgacaatgca gactgggaaa tcagcggcac cattactcaa
     7021 tattcattca gtacttcggc cattaccggt cagcaggctt ccaataaccg tttaacggtc
     7081 agtatctcga ttattctgca ggatcgtaaa gcggacaaaa cagaaaaaca tgaagtcagc
     7141 cggagttttg aattccgtgg cgatcagtca ttccagcagg ccgaaaccaa cctggccgag
     7201 gaaatgatcc ggaccctgac cgatgatatt ttcaccaaac tattttcgaa atggtaagcg
     7261 ggaaacactg ttccctcttt cccggaatca acgattcact taacttgcag gcatgaacgt
     7321 cagtatcaat caattggtaa aatcgctgct tcagaaagaa tccctggagc attgcagcct
     7381 gccggaactg acggcctatg ctgaacggaa tccttatttt ggcgcggccc aattactgct
     7441 ggcaaaaaaa ctccagaccg aatccccgga aagatttgaa gaacaatacc agaagaccct
     7501 gctctatttt cataacccgc tttgggtgga gcatttgctg aatgatacgg gtggggcgga
     7561 gattgaagag gttgaaaagg ttgaaaggtt gactagttta caggttgaca aggttgaaga
     7621 ggttcaagag gttgaagagg ttcaagaggt tgaaaaagtt caagaggttc aagaggttga
     7681 agaaggagct gaaaggttga ctagtttaca ggttgacagg gttgaagagg aagttgagaa
     7741 ggaaattata ttagatgaag aaaagatgga gactggagat aatatgaaca gttttgagac
     7801 agcggttgtg gaggaagtga gggccgatgc gccggttatt ccggtgcctg tgccggtgga
     7861 agcggtaaat ccggcaaatg acctggtttt tgagcctttt cataccgtgg attattttgc
     7921 ctcccagggt ataaagttta aggaggaaga acggccgact gatgagttcg ggaagcaact
     7981 gaagagtttt acagactggc tgaagaccct taaacgtctg ccggccactg aattagccaa
     8041 gaatatcacc ccccaatccg agaataaagt ggaacaactt gctggtcagt ccatcaccga
     8101 ccgggaaata ctgaccgaag ccatggcgga ggtatgggaa aaacagggaa atgcgctaaa
     8161 agccatggag atataccgta aattaagttt gttcgaaccc gctaaaagtg cttattttgc
     8221 agcgaaaatc gaaggattaa agaaaaaaaa ctgatatgac gactttattt gtgattctta
     8281 taattgtggt ttctgctgtt ctgggtctta ttgtgctggt tcagaatcca aaaggtggcg
     8341 gtttagccgg aaatgtggca ggttttagca accagttaat gggggtgaaa caaacgaccg
     8401 atgtgctgga aaaaggcacc tggatctttg gtggccttat tgcgctgctt tgtttactgt
     8461 ctgtattctt tatttcaaat accacaacag ccaatgcttc aggtcctggt gttgatccga
     8521 ccaaggtaac ggtgcctgtt cagcaacagc cgcagcctgg tactaatctc caaccaactc
     8581 cgactccggc tcctgtaccc ccggctaaat aataacaacg tactatttat acaaaacccg
     8641 gtctgcttat taaaaggacc gggtttttta tttaaattga tcccactatg aaaaagaaaa
     8701 tcatcctggg taccctcgtg gttctgcttt tgctcgcagg ctttatcggg tacaaagtgg
     8761 ccggtccggc tgtctccgat aaaaacggga aatacctgta tatccgttca ggtgatggaa
     8821 tgaaggaact cctggaaaaa ctggatacgg gtaaagtgat cagcagcaaa gcctggtttt
     8881 cacgggtagc gggttggctg aattttaaat ccgttaaacc cggccgatat gaattaaaga
     8941 acggaatgag tctttatgcc tttgtccgca aactcaggtc cggcgaccag tcactggtaa
     9001 aaatggtgat cgtaaaagaa aggacccgtg aagcctttgc cagtaaaatg gggcggaagt
     9061 tcgacaccga atgtgattcg ctcacgatga tccgctttat taacagtaat gactcactcc
     9121 aatccttcgg actggacacc agcacttttc tgtcggttat gcttccttat acctacgaga
     9181 tcagatggaa cagtacacca gcccggatca ttgagcagtt tcaggtggct tataaaaaat
     9241 tctggactcc tgaacgtacg gctaaagcgg ctcaattgaa cctgacgccc ctgcaggcaa
     9301 ccatccttgc ttcgattgta gaggaagaaa ccacccgtaa agcggataaa ttgaatattg
     9361 ccagcaccta cctgaaccgg ttaaagatca atatgaaact acaggcctgt ccgacggcca
     9421 aatatgtcag ccgtaatttc cagctcgacc ggatcacaga gaaacaaacc agcctggtat
     9481 ccccctataa tacctatctc aatgaagggc tgcctccggg gccgatctgt acgccttccc
     9541 ttgaatcaat ggaagcggta ttgaatgccc cggcaacagc gtacctttac tttgtggcga
     9601 gttacgcttt tgacggcagc actattttta ccagtaatta tgatgaccat atgaaatatg
     9661 caagaatgtt tcaggcggag caaagaagaa gggctgattc tgtgaaaaaa gcgcggagta
     9721 aatagtatgc cgctactgga taaaatactg caccggattg aaacactgcc attggtctct
     9781 tttcttatca aaaaaagcaa gaccacttcg ttgcctggtt ttaatgggat tgcgatttat
     9841 gatgtgatcc gttttttttt cggccaggta aagacaattg gtatgacaga aagagcttcc
     9901 gctgtcgcct ttaactttgt gatggcgatt ccgcctgcta tcatcttcct ttttacctta
     9961 attccgttta ttcccatcac cgatgaattt caacaggaaa tgttcagcct gatccgggat
    10021 gtgatacccg gtgaaaaaga caatgcggtc gtgatccact tcctggaaga ctttattaat
    10081 aatccccgca atggattatt atcgctcggt tttgtgttgt ccctgttttt ttcttccaat
    10141 gccatgatgg gcattatgcg ttcatttgat aaaaactata tcggcttcaa aaaaagaaac
    10201 ggattacaga cccgcctggt ggccatcaag atcacgctta tactttatgt gtttgttttc
    10261 gtttctgtgc ttttactggt tgcccgggta gccgtattaa aatggctggg gatcaccgac
    10321 gaaaccgtcc ttgccgtgat caataacctg cggctgctgg tggtggtcct gctgttcttt
    10381 gcttcgattt ccattattta ccgctatgcc ccttctgtac ataaaaaatg gaaactgatc
    10441 aaccctggct ccattctggc ttctttcctg atgttgctga tgaccttcgc cttttcctgg
    10501 tgggtcacca atttcagcaa ctataaccag ctttatggct cgatcagtac catcctgatt
    10561 atcatggccc tgattttcat caactccctg gtcctgctga tcggttttga actcaatgtg
    10621 agtatctcct ccctccgtaa aatggcggat gaacggaaag ataacgggac cactgaagaa
    10681 ggaatatctg cctgattgcc gaactttaag gtctaaacca ttgaaacatg aaaaaaatcc
    10741 tgtatgccgc cttcccggct atcctgctta tttcaggatg gctgaatacc gcagacccac
    10801 ttcctatcgg agccgctctg ccccaacctg aagtaaaaat gaaagatatc agtggtcagg
    10861 aagtttcttt cagcgatgca cggaaaaaga acgggctgct ggtgatgttt tcctgtaaca
    10921 cctgccctta tgtgatcaaa aaccagtccc gcaccaaaga agcctgcgct tttgcttccg
    10981 ctaatgagat cggggtggtg ttactaaact ccaatgaagc tcagcgggat ggggatgatt
    11041 catttgaaga aatgaaagcc tatgcggccg aacaggggta taactggtat tacctggtcg
    11101 acaaaaactc ggtcatggct gatgcctttg gcgccaaccg caccccggaa tctttcctgt
    11161 ttgacaaaga tggtaagctt gtttaccatg gggcaatcga tgataacgcc gctgacgcat
    11221 ctgccgtgag ccgcaaacac ctggttgtag ccatgacgga aatgaaatcc gataaagatg
    11281 tttcggtaaa aaaatcccgt tcagtgggat gtggcataaa aagacaataa ggtctgctgc
    11341 cctgaaaatt tgcatgtccg gcctggccgg acattttttt taactttttt tatggaaagt
    11401 gtaaagccct gcatccaaag aacaaaccac cttttcaaac ttaaatcatc gcttatgctt
    11461 tccagacacc aactcaaccg gatcatcgtg atcggcttta tggtacttgt gggctatgcc
    11521 ctggccaaag ctttttacca caacagtatt ttaggtatcc tgctaggatt aaccagtctt
    11581 ggtgccggcg tctatttcct ttacctgatg gccaaggcca aggaggaaat ggaagcagaa
    11641 gaaaatgcct gaacgaataa agtatagcca tgctttttat taaaccccgc ctgtgaaaat
    11701 tctttttccc tggctgttga tgctcttcat cagttgccag caaaaaaaca aagccggcac
    11761 gtcattattg cccgcattaa atgcggccgg acctatgggt tctgttgcag acagtccgac
    11821 tgtccgcaac agtatccatg tgtttaccgc actctgcgat aacaaatacc agggaatcgt
    11881 tccggttccg gccatcatgg gaaacggcca ggatccggcc aacaatctct attggggatg
    11941 gggatatggc atacgtacct atttcaataa aagtaaagaa tggaaattag ttacggtctc
    12001 cacaccgggt tatccggtac atgagcgatt ggtctataaa agcactattg gcgattacta
    12061 cctcgtagcc gatgcatatg atggccgggc catgaaggaa tgcctccagg atttcctgaa
    12121 agccctgtca ggaaatcata aaaaaacgat catcgccggc aatcagcagg tcggaatatt
    12181 tggtcatgcc cgcttgctgg cttttgtggg acataacgga ttaatggatt taacgctacc
    12241 cgaaacattt atacaggctg atagtctgaa gcgggaaggt attgtcctgg cttgttacag
    12301 taaaagctat tttagttccc ggtttaaaga cgctggtgcc tatcccctct tgtggaccag
    12361 taatctgttt ggcccggaag cctatagcct gcatgatgcc attagtggtt atatccgggg
    12421 tgaaaatgca gttgccatac agaccagggc agctactgtt tatgcaaaat tcacgaagtg
    12481 ctccgtccat gcagcgaagc gtttacttgt tacgggatgg tgaacggaac atttctttga
    12541 gtatatcctg taatgtccga tccgacagtt ttgcctccac aaatttccgg tatccatttt
    12601 ttttgtggag aaacagggtg gctggaatag ctccagacca tgagctatcg attaagggac
    12661 agaaataatc tgcattggtt tcatccagcc ataaaaacgt cccttcataa ttcctggcac
    12721 ggataaagtc cctgagtttc tccggaaaat tctctttgtc atccagactc accaggatca
    12781 actgcagact gtctgcctca tgcttcttca cttcccggat cagggaaggc aattcctcca
    12841 cacatggggt acaccaggtc gcccagaaat taatcactaa aggatgtttg ctttcgctca
    12901 tcaacttttc catgtccgta atcttaacac gacgaacctc ctgggcatcc aaacgaatcc
    12961 ccggcataaa aaacataacc cataatataa caagccctcc cttcattcct tacttttaac
    13021 taacgactaa tgactaacga cttccgacta ccgactacta actaccgact tctccctcct
    13081 ccctcagctc ccactccctc agcaacccct gtttcatttc atgttcaaag cccgggcgtt
    13141 cataatgacc cgcgagggtt ttctgccgga aggcttcgta taaagtaacc gcacaagcca
    13201 cactgatatt gagtgagcgg ataataccca cctgtgggat aataaaattg ccatcggcca
    13261 tggcccggat ttcatcactt accccactgt gttcattacc gaaaaccagg gcaatcggct
    13321 cggtcatatc aatatcatgt aagctgaccg catcactact caggtgagtg gtcagtatct
    13381 ttttatattt tttccggaga gcggaaaaac aagcagcggc atcttcaaac tgatagatag
    13441 tgagccattt cgcggcactg gacgaactgc gggcacccca ttttttgtgc ggggggatcc
    13501 ggttgttgag aatatatatt tcctgtatcc ctactgcatc acaggtcctc attacagcgg
    13561 agatattgtg cggatcgaat acattttcca ggactacggt caggtcgggc tgacgtttgg
    13621 ataaaacctg taaaagttta tggctgcgtt caggagtcat gcggggaact gtttcctgca
    13681 aacttcgtta ttttagttat ttttaatccg attgtttatt acaaataccg gtgaaaatga
    13741 aatatcctcc cgtccagaat tttattaatg gtgcatttat cactcctcaa acccaacggc
    13801 atttgccggt gatctcgccc gtaaacgggc aagttttgag tgaagtcccc ttatccggag
    13861 ccattgacct ggataaagca gtcactgcag catctgcggc ttttcgtggg tggagcagga
    13921 ctcctatcaa ggaaagggtg caggtttttt tccggtataa aactttattg gaaaaacacc
    13981 tggatgaact ggctgcccta tgcagtgaag aaaatggtaa gacccatgct gaatccgtgg
    14041 cagaaataga aaagtgtatt gaacttactg aattcgccac ctccctgccc cagttggtaa
    14101 cgggtgaaat actggaggtc agtaaaggtg tagagtgtcg tacagagcat gtacccctgg
    14161 gagtggtggc ctctgtcgtt ccttttaatt ttcctgccat ggtacccaac tggaccatcc
    14221 cgaatgccct ggcattggga aactgcatga tcatcaaacc ttctgaaaaa gtgcccctga
    14281 ccttgggccg gatagccctg ttactgaaag aagccggatt accagacggt gtttttaata
    14341 ttgtgaatgg agatagtgaa atcgtaacag ccatttgtga tcatcccggt attgaagccg
    14401 tttcatttgt cggatccacc cgggttgcca aacttgttta ccaacggggt acttcccatt
    14461 taaagagagt actggctctt ggaggagcca aaaaccacct gatcgttttg ccagatgcca
    14521 ataccgatat gacggcacag aatgtggtag ccagcatgag tgggtgtgcg gggcagcgtt
    14581 gtatggctgc aggtgccatg ttagcggtag gaaatgtgga ggccatcatt gaaaagattt
    14641 gcatcgaggc ccgtaaagtt attccgggaa aaaacctggg tgcagtgatc agtcaggatg
    14701 cgaagacccg gatcgaacag tatattacag aagctgaaaa acaaggtgcc aaaattctgg
    14761 tagatggacg cggggtgacc gtacctggta atgaagaagg tacctatgtc gggccgactg
    14821 taattgatta tgtgaggccg gagatggcag tagcccggga agaaatcttt ggtccggtta
    14881 tttctatcat acgcacccaa actgtggatg aagccctcgc cattgaaaat gcgagtcctt
    14941 acggaaatgc cgccgctgta tttacccagc atggaggcat ggcccggtac attattgagc
    15001 gggccagtgc cggtatgatc ggggtcaatg ttggagtacc cgttccaagg gagcccttct
    15061 cctttggtgg ctggaatgaa agcaaattcg gtaccggaga tattacaggc aaaagttcga
    15121 ttgctttctg gacgcggctg aaaaaatcca ccgtaaaatg gaacccagaa gccgggatca
    15181 actggatgag ctgatcgcgt ccagacataa cggttttccc tattccagat ttatggcggt
    15241 gatactgcta tattccggta agtgctacca cttaatttgc aatcagtaaa tcaagatcag
    15301 gggaatttgc cggcagaact gcgtaaatta tggtaagaat tttaaatatc ttaccatgtt
    15361 gaatttttat tctgccagta cccggatcgt aaatacgcgc cggggaatca cggagtgctt
    15421 ggaatctgca ctcggcagta atacaaccga ctgtgattta gtcattatcc atgcatcact
    15481 tggtcataat tttaaagaac tcatagagga ggcgcagttg cttgcgccgc atgcaaaagt
    15541 agtagcagcc tcctgttgtg gcattgtggg caaggaaggg gtcagtgaaa ccatgaaaga
    15601 catcgccatc atggcggtca ggggaaaaga attcggactc gcctgggtca atgaaatttt
    15661 cgggaataac tcctatgaaa aagcaaaaga aatggcggaa gaactctatc gccagcaacc
    15721 ggatacccgt ttcatttatt tcctggcttc aggaattgat attgccaatg accgttgtat
    15781 tgaaggcatc gaatcggtat tcggacctga agtaacaata ttcggtgcca catctgccga
    15841 taatatgaaa ggggtggtaa gttaccaggc tgttgccggc caggtatttg aacatgctgc
    15901 tttcatgatt ggttttgccg atcccacact cgaagtgaat acacaggcaa cccatggatt
    15961 tgtagctata ggagaaccct ttgtagtaac acgtgcggaa ggacattaca tttatgaatt
    16021 gaacgggaaa cctgcctgga cagaatttac acggcacctc ggacttccag ccactgccac
    16081 ttgtggtgat tccattccta tcggcgccct ggctgaaaaa ctgagccctg aattgtccaa
    16141 agaatatggc aatgatcata ttcttcgggt ggtgacccgt catgaaggag ataccatgct
    16201 ttatgccacc acttgtaaaa ccggaactga actctggctt acgttgcgtg atgag
//
LOCUS       DAOPMW010000036        15573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3746_length_15573_cov_5.924797, whole genome shotgun sequence.
ACCESSION   DAOPMW010000036 DAOPMW010000000
VERSION     DAOPMW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15573
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_15573_cov_5.924797"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLU11_03395"
     CDS             <1..247
                     /locus_tag="PLU11_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58186.1"
                     /translation="LQPLFKLFLYTTDKLEISIRQTGLDKYSIRLLNIDMALPVEIKT
                     DEGIKKIMVDKKGVSVSSKTFVQVDPRVFYLKKLIYE"
     gene            294..581
                     /locus_tag="PLU11_03400"
     CDS             294..581
                     /locus_tag="PLU11_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58187.1"
                     /translation="MRNQPEIEFFIYFSREHDSRRYRAKVKVLYRTENVIRFSIRAGE
                     KAMEMEKFLYRKTNRWKVEQMNFIRLGHTRVYEKMISGIQVAIDEEMKKYP"
     gene            649..999
                     /locus_tag="PLU11_03405"
     CDS             649..999
                     /locus_tag="PLU11_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58188.1"
                     /translation="MTPAARSVCYFGFYLYGVGLTLMAIPNLFLKTLQLPETQEVWIR
                     ILGVVVFGLGFYYHRSGVQNNTAFFKLTIPTRIFVFISFVSFVLLGYAAPIMIAIGAV
                     DLLGAAWTYSALKK"
     gene            complement(1015..3594)
                     /locus_tag="PLU11_03410"
     CDS             complement(1015..3594)
                     /locus_tag="PLU11_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN58189.1"
                     /translation="MRTHKYVIFLLFFLFFIFAFFLWEFKSINVNNRQNVDAVETTTR
                     FSKNLNQLRFELLKTDNNIISGIYKPAQKLHYDFKTDSTSLIRLFNEPTFTKIAEPSL
                     NKSLTTLRGKVEEKLTLQSAYVANAPADSLSGLVYNKKNEALNKSIHSGLTSVNTLQQ
                     KLFSVQIGKVEDTYTKQYLFALGFAILSFLVFYFLLTGLNSHIQRRKAAEEEAIINEE
                     KYKNIIDESGAIIFTTNMDGRFTFVNEMACRVTGFAQDELLKKSYIDLVHPIWQDQLL
                     HFYSDALHKGDLETQIKFPITCKNGDMKWVEQTAVIVFQQGQPMGFHCVTKDITESYQ
                     LEREKKYLYQLFQSIMDNSPQAIFIKSTEGRYIHVNKQFCELLMLTELEIVGKTDDEL
                     FGKSLTTHYRNKEAQVIKTQTPSRLRQNFNSKKGIRTFSIEIFPLFDHNSEVLGVSGI
                     ASDITDAVQNQRNLISALKRAESAEKHTQSFLANMSHEIRTPLNSIIGLSYQLLKSDM
                     RQKEHGFVRTIHSASEHLLVLINDILDTSKIEAGKLGLDQTSFSLRELIQLMVELLRQ
                     KAEEKNIRITTEIDSNLFDSHIGDPHRLKQILLNLLTNAVKFTPKGLIRITCKVEEDE
                     AKQQSIALTIIDTGIGIEQEYIDRIFTKFSQEDKSTTRNYGGTGLGMFITKSLVELMG
                     GTISVSSQKEIGTHVKINLSLPKNLVPVIQLPAKATTDTSILNNLHILVVDDNEMNRL
                     VASTVLENHGIIVSEAVNGADAIRKLEKESYDLILMDLQMPVMDGLEASLKIRKDLGL
                     SVPIVALTASALNEESHRCLDAGMNDFIIKPFKEEEMVQVLIRNIRLDHRSELSDHVR
                     KIS"
     gene            complement(4261..5349)
                     /locus_tag="PLU11_03415"
     CDS             complement(4261..5349)
                     /locus_tag="PLU11_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58190.1"
                     /translation="MKKLYLLLLLNAPLWVAAQKNVDLDKYRFTVQYRALPQMQLDST
                     YRTYHVEIESTRLMESFMDDLSPENSVKLEGWKKLEQDGHIGIQVKLGDLLPESFSVK
                     ERLETVRNGNGIVTGSRTYYHQEVVYSFSANASIADFKGMHIMDQELISRQNKQVYKS
                     PEFNIKHVAEGYFLVNSMSVTRELYRNAVTRAMHTLSDRITRNFGFDEVAVNDYMWII
                     DSRKHPEYSAWRNAFSQMNEVLYNMDANTPITGAREKLQPVISYFEDIRKRYSSTKKH
                     DRKIRYATYFNLAVLYYYLDDPQSMMREANGLVLNDYDSRDGKGFEETAAWLKNQFLQ
                     NNIYTRHFPVNPGSYRGPMEKKDISSIN"
     gene            complement(5566..6258)
                     /locus_tag="PLU11_03420"
     CDS             complement(5566..6258)
                     /locus_tag="PLU11_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58191.1"
                     /translation="MFFSDSMAQDCKVLEDSLKGTYEGECKKGLANGNGTAAGTDRYT
                     GHFKNGYPDGNGKYTWKNGDWYEGEWKKGKQNGMGTFSHRVINGQGQTETLTGFFKNG
                     EYKGKFEKPYSTEMLTNNFTSISVKKMNTLVSEITFTVKNTTAGGSTNSQPVLPKSEI
                     KNIQLLQGNYTEMIYDTTSQVTNRYIFRNIIYPFSAIITFERARSVQQVAIAKIELNE
                     NGNWMIRVDVEQ"
     gene            6423..8972
                     /locus_tag="PLU11_03425"
     CDS             6423..8972
                     /locus_tag="PLU11_03425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPN58192.1"
                     /translation="MAKGLSTAEAKAALQSHGFNELLTARPKNVLKIALEVVREPMFL
                     LLISCGLLYMILGDYREGIIMLSTIFLIIFITFYQYRKTEKALDALRNLSSPRALVIR
                     DGKELRISGREVVPGDLLVLNEGDRIPADAFLLESLNLTIDESLLTGESAPVTKLTGA
                     VPDEEKITVFSGTLVVQGNGLAEVTATGNRTAFGKIGVSLAAIQQEETQLQTEMKKLI
                     RNLFLIGAVLSIAIVLLFYFTRGNFLQSLLNGLTAAMAILPEEFPVVLTVFLALGAWR
                     LSRRNVLTRKSSAIETLGSATVLCSDKTGTITLNKMEVTVVYNGKDIYHSDVFSEHHA
                     LFYNLIKTASQASNEKPVDPMEIAIGELYDKLYDTGTSGAAFIREYPLSRELMAMTRV
                     MQDPMGESSPYQVYAKGAPETILKMCKLEAATQIQHEKVLKQLAAEGYRVIAVASAEH
                     APSSLPDEQEQFTFTFIGFVGLTDPVRPEVPQAIKDCYTAGIRVIMITGDYPVTAASI
                     ARSIGLAPEGEIISGEELVKMSDEELKVRIGRVTVFARVVPEQKLRIVEALKANNEIV
                     AMTGDGVNDAPALKAAHIGVAMGMKGTDVAREASSLVLLDDNFTSIVAAIRLGRRIFD
                     NLQKAMSYILAIHIPIIGLTLLPSFVSTLPLLLMPLHIVFMELIIDPVCSVAFESEQE
                     EKGIMNRPPRKRDDRFFGSRKILQSFFSGLLLLLMVLFVYFFSIHEGHTEGEVRAIAF
                     SALITGNIILILTNLSKSRTALAVLAEKNTALLIILGSAFIMLQLILFIPGLRKIFSF
                     DFPGMSHFIPSFIGASLVLLILETVKYFKYRRKSKLEKNREGLKVVSGNSV"
     gene            complement(8944..11160)
                     /locus_tag="PLU11_03430"
     CDS             complement(8944..11160)
                     /locus_tag="PLU11_03430"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN58193.1"
                     /translation="MCIRYSFLFIFILSVFRLSAQDAAVFRPDSTQREIKALPIRQSI
                     KLDGKLDEAEWKLAGLSPLFTQIEPLQGQAPNFATSVKVLYNRQFLYFGVFCRDSVGK
                     KSIRATDFKRDFNTRQHDHIGLSFDGFNDQRNSMALFTNAYGVQRDLLNFDDMYTDLD
                     WDGLWKVRTTRTDSGWYAEMAVPWQTLRYPRKNDSLQQWGFNIYRNRRLSNEISAFSG
                     YPRSFSFLRMAYSGKLTNLEPPPPGRNLRIQPYILSSYDRYKNNTSITDPEKTAFRYG
                     GDIKWAITPHSILDLTFNTDFAQADADRQVNNVTRFSVFFPERRQFFLENASLFGTGV
                     APGEDLSGGSMRIQPFFSRRIGLDDSGNPIPIDAGGRFVYRSAKNNIGAMLMHQQGSS
                     IMPATDFFVGRFSHNLGRLNRIGGLVTARNNSQGHNIVSTMDGFFRLSESHQLNMMVS
                     HSSTSATGKQGLSGFAQYYYVNNQWKFWWTQSFVSKNFNPEMGFVSRNDVIATTPGIF
                     WYYRGKNLPFPKLIRAFEPGAWFEMYHQASTGQLTERTVSLNPVWFNFQQGGYIGYIV
                     NHSFQRLPETFSPVGIDILAGDYNYFNHQIYYSTDPSRKISGGLDLTFGDYFNGKLTS
                     TNTRLQFSPSPHFSLLAQYNRLNFDEVGTPAISKKTDLYTVSGRFALNPRIQLIGFYQ
                     LNSENNLGNYNLRFSWEYQPLSYIYVVFNHRGFDNALAKRQTEDHSIIKLSYLKQL"
     gene            complement(11334..11765)
                     /locus_tag="PLU11_03435"
     CDS             complement(11334..11765)
                     /locus_tag="PLU11_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58194.1"
                     /translation="MAAENLSNYTPVKAITLERLVHALINVKLESVVRKQSFFINEVH
                     SNVPLNADRNVVTNVLDSLLQNVLNETSSSCIRISAKEYSQVLLIHIYDNHEADYGAE
                     NVVSDLVRVEAQKIGGFVGITRHQKNQTVIAFSFPNVPLAA"
     gene            complement(12280..13488)
                     /locus_tag="PLU11_03440"
     CDS             complement(12280..13488)
                     /locus_tag="PLU11_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPN58195.1"
                     /translation="MKLIPLALLAFFLHFCGSPRQVQTEKEIRILTGADQTEKYIPLL
                     QGKRVAIMANPTTVIGSTHLVDSLQKRGVNIVKVFGPEHGFRGNASAGVHVADETDPV
                     TGIPVISLYGSKNKPSKQDLADVDILLYDLQDVGCRFYTNINALARLMDACYENGKEM
                     LILDRPNPNGYFVDGPVLDMKFKSGIGMFPIPIAHGLTVGEFARMVNGEGWLTNKVKC
                     PVTIIPVANYNHDMPYTLPVKPSPNLNTQQSILLYPSTCLFEGTYLNHGRGTYFPFTM
                     LGSPSLKGKYDFSFTPTGIKGMSETPLFMNQLCYGLDLRNYDVSQLRKTKQINLQWMI
                     ELFKASPNKEQFFDNKLSKEMGVIEKLTGHASFRQQIMEGKSEKEIRASWEPGLSEYK
                     EMRKKYLLYP"
     gene            complement(13637..14137)
                     /locus_tag="PLU11_03445"
     CDS             complement(13637..14137)
                     /locus_tag="PLU11_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN58196.1"
                     /translation="MTFTASLLKEMENEARTTRKILERVPDGNYDWKPHEKSMSIRQL
                     ATHVAEITGWIPLILHTDEMDFTKMEYKPAAINNQQELIAHFEKSLSEGTSALSAVTD
                     DKLVNETWTMRSGDIIHSADSRLENVRHCFCQIVHHRAQLGVYLRLLNIPLPGSYGPS
                     ADDTSF"
     gene            complement(14204..14893)
                     /locus_tag="PLU11_03450"
     CDS             complement(14204..14893)
                     /locus_tag="PLU11_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPN58197.1"
                     /translation="MNRMDRLLGMLTLLQSRKYVTAEKIAAKFSISIRTVYRDVKALG
                     ELGIPVSFEAPKGYFIVQGYFLPPVSFSTEEANALLLIEVLVNGFTDQSVRKHYGSAL
                     DKIRSVLRGHQKEQLELLNDKIRFQKPACYDLHFEYLPSLQNAIAARSVIELEYLNQK
                     EETSKRKVEPIGLIFYAFNWHLIGWCHSRRDYRDFRVSRIKKFTDTGLPFRKKEHMEL
                     NEYMKQLPVNY"
     gene            complement(14954..>15573)
                     /locus_tag="PLU11_03455"
     CDS             complement(14954..>15573)
                     /locus_tag="PLU11_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPN58198.1"
                     /translation="LAASIIQAQETERTRWAEELHDNVCQILSVANMYAIDVTDHPEN
                     LPSLGPQLKKLVAESINEIRQLSATIRTPAFDQETLVESIEKLSANINRLKPVTIELD
                     NQSLNEGELENDKKLMIYRVVQEQINNIIKYAEARSVEIKLDNTSSNQVLISVKDDGK
                     GFDPLKVKTGIGLRNIQSRLQVYNGNIAIHSSPGNGCLLEASFPN"
BASE COUNT         3919 a         3302 c         3737 g         4615 t
ORIGIN      
        1 actccagcca ttgtttaaat tatttttata taccacggat aaactggaga tcagcatccg
       61 tcagaccggc ctggataaat attcaatccg gctgctgaat atagatatgg ctcttccggt
      121 agaaatcaaa acggatgaag gtattaagaa aatcatggtg gataaaaagg gggtttcggt
      181 cagcagcaaa acttttgtac aggtggatcc ccgggtattt tatctgaaga aactgatcta
      241 tgaatagcag ggtctgaatt ggtttatagt tgaatagtag tgaagagtga tgtatgcgta
      301 accaacctga aattgagttt tttatttact tttcccggga gcatgattcc cgcaggtacc
      361 gtgcaaaggt aaaagtgtta taccggactg aaaatgtgat ccgtttcagc atccgggccg
      421 gggagaaagc gatggagatg gagaaattcc tgtaccgcaa aaccaatcgg tggaaagtgg
      481 aacagatgaa ttttatccgg ttaggtcata cacgtgttta tgaaaaaatg atcagtggta
      541 tccaggtggc tatagatgag gaaatgaaaa agtatccctg acatacagtc ggctgtacgt
      601 tgtttactac ggatttatca gactatttat aaacaaaaac caacatttat gactccagca
      661 gcacgttccg tttgctattt tgggttttac ctttatgggg tgggacttac cttaatggct
      721 atccccaatt tgtttttaaa aactttacaa ttaccggaaa cgcaggaagt atggatcagg
      781 attttaggcg ttgtggtctt tggccttggg ttttattacc accgttccgg ggttcagaat
      841 aataccgctt tttttaaact gactattccc acccggatct ttgtctttat ttcttttgtt
      901 tcatttgttt tgttgggcta tgctgctcct atcatgattg ctatcggtgc tgtggatttg
      961 ctaggagccg cctggaccta ttctgctctg aaaaaatagc cattcagttt gtcttcagga
     1021 tattttccgg acatgatcac tgagttcgga acgatgatcc agcctgatat tccggatcaa
     1081 aacctgcacc atctcttctt ccttgaaggg tttgatgata aagtcattca tacctgcatc
     1141 caggcagcga tggctttctt cattcagggc actggcggtc agggcaacga ttggaacgga
     1201 gagcccaagg tctttcctga ttttaaggga ggcttccagt ccgtccatca ccggcatctg
     1261 caggtccatc aggatgaggt catagctttc tttctccagt ttgcggatgg cgtccgcccc
     1321 attaacggct tcactcacaa tgattccatg attttccagt acggtggaag ccacgagccg
     1381 gttcatttca ttatcatcaa ctaccaggat atgcagattg ttcaggatac tggtatcggt
     1441 agttgctttt gcgggtaact gtataacggg taccaggttt ttcggcaggc tgagattgat
     1501 ctttacatgg gtgccgattt ctttctggct gctcactgaa atcgtgccgc ccatcagttc
     1561 caccaggctc ttggtaataa acatacccag tccggtgccg ccataattgc gggtagtgga
     1621 tttatcttcc tgggagaact tggtaaagat gcggtctata tattcctgtt caatgccaat
     1681 gccggtatca atgattgtaa gcgcaatgga ttgttgtttg gcttcatcct cttcaacttt
     1741 acatgtaatc cggatcaggc ctttaggggt gaattttacg gcattggtca gcaggttcag
     1801 tagtatttgt ttcagccggt gcgggtcgcc gatatgactg tcaaacaggt tactgtcaat
     1861 ctcggtagtg atccggatgt ttttttcttc tgctttctgg cgtagcaatt ccaccatcag
     1921 ctggatcagt tcccgtaagg agaaggaggt ctggtccagt ccgagtttac ctgcttcgat
     1981 tttcgaagta tccaggatat cgttaatcaa aaccagcagg tgttcagagg cggaatgaat
     2041 ggtgcgcaca aatccatgtt ctttttgccg catgtctgat ttgagtaact ggtaacttag
     2101 cccgatgatc gaattaaggg gcgtgcggat ttcatggctc atattggcca ggaaactctg
     2161 ggtgtgcttt tcagcggatt ctgctctttt gagtgcgctg atcaggttgc gctgattctg
     2221 tacggcatca gtaatatcgg acgctattcc ggaaacgccc aatacttcgc tattgtgatc
     2281 aaagaggggg aaaatctcta tggagaatgt ccggatgcct ttcttgctgt tgaaattctg
     2341 acgaagccgg ctgggggtct gtgttttaat tacctgtgct tccttattcc ggtaatgcgt
     2401 tgtaagactt ttgccaaaga gttcatcatc tgtttttcca acgatttcca gttcggtcag
     2461 catcagcagt tcgcagaatt gcttgttgac atggatatac cggccttcgg tggatttgat
     2521 aaagatcgcc tgcggactgt tatccataat ggattggaaa agctggtaca ggtatttctt
     2581 ttctctttcc agctggtaac tttccgtgat gtctttggtg acacaatgga agcccatggg
     2641 ttgtccctgc tggaatacga tcacggcggt ttgttccacc catttcatat cgccgttctt
     2701 gcaggtaatc gggaatttga tctgtgtttc caggtcacct ttatgcagtg catcgctgta
     2761 aaaatgcagg agctggtcct gccagatggg gtgaaccagg tcgatatatg attttttgag
     2821 gagttcatcc tgggcgaagc cggttacccg gcaggccatt tcattcacaa acgtgaagcg
     2881 tccatccata ttggtggtaa agatgatggc gccggattcg tcaataatgt ttttgtattt
     2941 ttcctcattg atgatggcct cttcttccgc tgctttcctt ctttggatat gggagttcag
     3001 cccggtgagc aggaagtaaa ataccaggaa ggaaaggatc gcgaaaccaa gggcaaaaag
     3061 gtattgtttg gtatacgtgt cctccacttt gccaatctgt acagaaaaca gtttttgttg
     3121 caacgtgttt accgatgtaa gaccggagtg aatagattta ttgagggctt catttttttt
     3181 attatagact aatccgctca gactgtctgc cggagcattg gctacataag ctgattgcag
     3241 ggttagtttt tcttccactt taccccggag tgtggtaagg cttttgttaa gtgagggttc
     3301 agctattttg gtgaacgttg gctcattgaa taaacggatc agcgaagtgg aatcggtttt
     3361 gaaatcatag tgcagctttt gtgcaggctt atagatcccg gaaataatat tattatccgt
     3421 tttcagcaat tcaaaacgta gctgattcag gtttttggag aaacgggtgg tggtttccac
     3481 cgcgtctaca ttctgacggt tgtttacatt gatactcttg aattcccaca gaaaaaatgc
     3541 gaaaatgaag aacaggaaaa agagtaagaa tatgacatac ttatgggttc tcatcggggt
     3601 agggattcgg atttatgaac tactaacgac taaagtttat cattttgttg cttaattgcc
     3661 aatatgttgg caggattaaa atcattttat tggtttcctc ttagtccggt atagtggtaa
     3721 atttctcatt tcggaaaacc gtctaagtgt ctgcggaact tttcgtagta gaaacaacct
     3781 tgtttttcag taagctatgg gtttttagcc tcttcccgtt tgcagcaata tttgtaatat
     3841 tctgtggact tacttagttc attttattta tttgaagcat gatttatatc atatgtaagg
     3901 gcatgcccgt aatcctatat tacggttagt aagaaagcga gattaaaaac gaaagcccat
     3961 ggaactactg ctcatatacc cggatttttt catctgacaa tgcctgtgta atcccaggcc
     4021 agactgatat tcatcgtaaa aatactttaa gcgctataca gcgacaccct gtatctatgg
     4081 atgttacagc aggtagggtt cacgcggagg gtaaggccta tggaatgtac atctgtcggt
     4141 tgtatacacc tgcaggcgtt ttcagcatca gctgatcctg aaagggtgag ggttgtttct
     4201 attataaatg aaagggctgc atgctatgca gcccttttcc atacatatgg cagatgcggt
     4261 tcagtttatg ctggaaatat cttttttttc catgggtccg cggtatgagc ccggattaac
     4321 cggaaaatga cgggtgtaaa tattattttg caggaactgg tttttcagcc aggctgcagt
     4381 ttcttcaaat cccttgccat cacggctgtc ataatcattt aataccagcc cgttcgcttc
     4441 ccgcatcatt gattggggat catccaggta ataatataga accgcgagat tgaaataggt
     4501 ggcataacgg attttccggt catgtttttt tgtggaagaa taccgtttac ggatgtcttc
     4561 aaaataactg attacaggtt gcagtttttc cctggcaccg gtaatgggtg tgttggcatc
     4621 catgttatac aacacttcat tcatctgcga aaaggcattt cgccaggcgc tgtattccgg
     4681 gtgtttacgg ctgtcgatga tccacatgta atcattaacg gctacttcat caaagccaaa
     4741 gttccgggtg atccggtcgc tgagggtatg catggccctg gtcaccgcgt tgcggtacaa
     4801 ttctcttgta actgacatcg agtttacgag aaaataccct tcagccacat gtttgatgtt
     4861 aaattcggga cttttataaa cctgcttatt ctgacgggat atcagttcct gatccatgat
     4921 atgcatgcct ttgaagtcgg cgatactggc attggccgaa aagctataca ccacttcctg
     4981 gtgatagtaa gtccggctgc cggttacgat accattcccg tttcttacgg tttccagtct
     5041 ttcttttacc gagaaagatt cgggtaatag atcacccagt tttacctgga ttccgatatg
     5101 cccgtcctgc tccagttttt tccagccttc gagtttaacc gaattttccg gcgaaaggtc
     5161 atccataaag gattccatca accgggtact ttctatttcc acatggtaag tgcgataggt
     5221 gctgtccagt tgcatctgtg gcagtgcccg gtattgtacg gtaaagcggt atttatcaag
     5281 gtccacattt ttctgggcgg caacccagag cggggcgttc agcagaagta aaaggtataa
     5341 ttttttcaca gtcaggattt tgtctgtaaa aaacggaagc aaagcttaaa tagtacatga
     5401 tcagtagcag aatattgaag agggtaggca cgtttatcgt aaatacacga gggaaaacac
     5461 gaaagaagtt gttagctgtc atcccgatgc ttcgggagtc agtcaacccc gaaaatgaaa
     5521 cttccttgtc aacctattta cgtattaacg tatcaacctg ttccttcact gttccacatc
     5581 cacccggatc atccagttgc cgttttcgtt gagttcaatt tttgcaatgg ccacctgttg
     5641 tacagaacgg gctctttcga atgtgataat ggcactgaag gggtaaatta tattcctgaa
     5701 aatataacgg ttggtgacct gcgaggtggt gtcatagatc atttcagtat aattgccctg
     5761 gagtaactgg atatttttta tctccgattt tggaagcacc ggctgactgt tggttgagcc
     5821 tccggctgtg gtatttttga cggtaaaggt aatttcggat acgagggtat tcattttttt
     5881 gacactgatg ctggtgaaat tatttgtcag catttcggtg ctgtatggtt tttcaaattt
     5941 ccctttgtat tccccgtttt taaaaaagcc agtcaatgtt tctgtctgac cctgcccgtt
     6001 aatcacccgg tgactgaatg ttcccatacc attttgtttt cctttcttcc attcgccttc
     6061 ataccagtct ccgtttttcc aggtgtactt tccatttcca tcagggtagc cgtttttgaa
     6121 atggccggtg tagcggtcag tgccggcagc tgtgccattg ccgttggcca gtcccttttt
     6181 gcattcccct tcgtaggtgc ctttgaggga gtcttccagg actttacagt cctgtgccat
     6241 tgaatctgag aaaaacatac agaataatat aaacaaagca cagcttttca taaaagaggt
     6301 tgttttttaa agatagtcca attctccgga atcgcactat tgactggcat cagttattta
     6361 tctgaccatt atctgtgata accgcgatcg cttccgttaa ctttcaggta tatattttac
     6421 ccatggccaa aggtttgagc accgcggaag caaaggctgc tttgcaatcg cacggattta
     6481 atgaacttct tacggcacga ccgaagaatg tactcaagat tgcactggaa gtggtgcggg
     6541 aaccgatgtt cctgctcctg atcagctgtg gcctgcttta tatgatactt ggcgattacc
     6601 gggagggcat cattatgctg agtacgatct tcctgatcat ttttattact ttttatcagt
     6661 accggaaaac agagaaagcc ctggacgccc tgcgtaatct gtcttcaccc cgtgccctgg
     6721 tgatccggga tggaaaagaa cttcgaattt ccgggcggga agtcgttccg ggtgatctgc
     6781 tggtgctcaa tgaaggtgac cggattccgg ctgatgcctt tttactggaa tcacttaacc
     6841 ttaccattga tgagtccctg ttgactggtg aatcggcccc ggtgacgaaa ttaacgggtg
     6901 ctgttccgga tgaagaaaag attactgttt ttagtggaac ccttgtggta caaggtaacg
     6961 gactggccga agtaacggca accggaaata gaacggcttt tggaaagatt ggtgtttcgc
     7021 tggccgcgat acagcaggag gagacacagt tgcaaaccga aatgaaaaaa ctgatccgga
     7081 atttatttct gatcggggcg gtactcagta ttgctatagt gcttttgttt tattttaccc
     7141 ggggcaattt tttgcaatcc ctgctcaacg ggctcacggc agctatggct atccttccgg
     7201 aagagtttcc cgtagtcctt actgtattcc tcgcattagg tgcctggcgt ttatccaggc
     7261 ggaatgtact tacccgtaag tcatcggcca ttgaaaccct tggttctgcc accgttttat
     7321 gcagtgataa aaccggtaca attaccctga ataaaatgga agtgactgtg gtgtataacg
     7381 gcaaggatat ttatcacagc gatgttttta gtgaacatca tgcacttttc tacaatttga
     7441 ttaaaacggc cagtcaggca tcgaatgaaa agccggtaga tccgatggaa attgccatcg
     7501 gcgaactata tgataagttg tatgatacgg gtacttcggg ggctgccttt atccgtgaat
     7561 acccgttgag ccgcgaattg atggccatga cccgggtaat gcaggatcca atgggggaat
     7621 cctctcccta ccaggtgtat gccaaaggag caccggaaac tattttaaaa atgtgtaagc
     7681 tggaagcagc tacacagatt cagcatgaaa aagtgttgaa acaactggcc gccgaagggt
     7741 accgggttat tgccgttgcc agtgccgaac atgcgccgtc ttcattgccc gatgaacagg
     7801 aacagtttac ttttacattt attggctttg tcggactcac ggatccggta cgcccggaag
     7861 taccacaggc aatcaaggat tgttataccg ccggtatccg ggtgatcatg atcaccggag
     7921 attatccggt gaccgctgcc agtatagccc gatctatcgg gttagcgcct gaaggtgaga
     7981 tcatttcagg agaggaactc gtgaaaatga gtgatgagga attgaaagtc cgaatcggtc
     8041 gtgttacagt gtttgcgcgg gtagtgcccg aacaaaaact ccggatagtg gaagcgttga
     8101 aagccaataa tgaaattgta gccatgacgg gtgatggggt caacgacgcc cctgcattaa
     8161 aggctgccca tatcggtgta gcgatgggaa tgaaaggtac cgatgtggcg cgggaagctt
     8221 cctccctggt attgctggat gataatttca cttccattgt tgctgcgatt cggttgggcc
     8281 gccggatttt tgataacctg cagaaagcga tgtcgtatat tctggcgatc catattccga
     8341 ttatcggtct caccttatta ccttcatttg tatccaccct gccattgctg ttaatgcctc
     8401 ttcatattgt atttatggaa ttgattattg atccggtctg ttcggttgca tttgaatcag
     8461 aacaggaaga aaagggcata atgaaccggc ctccaaggaa aagagatgat cggttttttg
     8521 gatcacgtaa aatacttcaa agttttttca gcggtttatt gttgcttctc atggtactat
     8581 ttgtgtattt tttctcaata catgaaggcc ataccgaagg cgaagtcaga gcgatagcct
     8641 tctcggcttt gattaccggt aatatcattc ttatactcac gaacctatcc aagagccgga
     8701 cagctttggc ggtactggcg gagaaaaata cagctttgct gattatactc ggttctgctt
     8761 ttattatgct gcagttaatt ttgtttattc cgggacttcg aaagattttt agttttgact
     8821 ttccggggat gagtcatttt atcccgtcct ttattggagc ctccctggtc ctgcttattc
     8881 tggaaacagt caaatatttc aagtatagaa gaaaaagcaa actggaaaaa aaccgcgaag
     8941 gccttaaagt tgtttcaggt aactcagttt gatgatcgaa tgatcttcgg tttgtctttt
     9001 cgcgagtgca ttgtcgaatc cacggtgatt gaacaccaca tatatataag aaagcggctg
     9061 gtattcccag gaaaaccgga ggttgtagtt gcccaggtta ttttctgaat tcagctggta
     9121 aaagccgatc agctgaatcc gcgggtttaa agcaaaacgg ccggagactg tatacaggtc
     9181 cgttttttta ctaatggctg gtgtacccac ttcgtcgaag ttaagcctgt tgtattgggc
     9241 gagcagactg aaatgcgggg aaggggagaa ttgcagccgg gtattggtgc tggtaagttt
     9301 tccattaaaa taatccccaa atgtgagatc gaggccccca ctgattttcc gggagggatc
     9361 ggtactataa taaatctggt gattgaaata attataatca ccagccagta tatcaatacc
     9421 caccggggag aaggtttctg gcagccgctg aaagctgtga ttgacaatat atccgatata
     9481 cccgccctgt tgaaaattga accaaaccgg attcagggat acggttcgtt ctgtgagctg
     9541 tcccgtggaa gcctggtgat acatttcaaa ccatgcacct ggttcaaatg cccggatcaa
     9601 ttttgggaat ggcaaattct ttccccggta ataccagaat ataccaggag ttgtggcgat
     9661 gacatcattc cgggaaacaa atcccatttc aggattgaaa tttttagaaa caaaggattg
     9721 agtccaccag aatttccatt gattattgac atagtaatat tgtgcaaaac ccgagaggcc
     9781 ttgtttcccc gtggcactgg tgcttgaatg ggagaccatc atattcagct gatgcgattc
     9841 actcagcctg aagaaaccat ccatggtact cactatatta tgtccctggc tgttatttcg
     9901 ggccgtaacc aatcctccaa tccggttcaa tcttcccagg ttatgcgaaa atcgtcccac
     9961 gaaaaaatca gtggccggca tgatagaaga gccttgctgg tgcatcagca tcgcgcctat
    10021 attgttttta gccgagcggt acacaaaacg gccaccggca tcaattggga taggattacc
    10081 actgtcgtcg agtccgatcc gccggctgaa gaaaggttgt atccgcatac ttcctccgga
    10141 aagatcttcg ccgggagcca cacccgttcc aaagagagag gcattttcaa ggaaaaactg
    10201 acgacgttca ggaaaaaaaa cggaaaaccg ggttacatta ttgacctgtc ggtcggcatc
    10261 tgcctgggca aaatcggtat tgaaagtcag atcgagaata ctgtgtggag taatcgccca
    10321 ctttatatcg cccccataac ggaaagcggt tttttcagga tccgtaatac tggtattatt
    10381 tttataccgg tcatatgaag aaagtatata aggctggatc cgaaggttgc gtccgggcgg
    10441 tggtggttca aggttggtga gtttgccgga ataggccatc cgcaaaaaag aaaaggagcg
    10501 gggatagccc gaaaaagcac tgatttcatt cgataacctc cggttccggt aaatattgaa
    10561 tccccattgc tgaagactgt catttttccg gggatagcga agcgtttgcc agggtacagc
    10621 catttccgca taccagccag aatcggtacg ggtggttctg accttccaga gcccatccca
    10681 gtccagatcg gtatacatgt catcaaagtt cagtagatcc ctttgtactc cataggcatt
    10741 tgtaaataac gccattgaat tccgctgatc attaaatccg tcaaaggaaa gaccgatatg
    10801 atcgtgctgc cgggtattga aatccctttt gaaatcagtg gcccggatcg atttcttacc
    10861 aaccgaatcc cggcagaaaa ccccgaaata aagaaactgg cgattatata acacttttac
    10921 tgaagtagcg aaattgggtg cctgcccctg aaggggttca atctgggtaa acaggggaga
    10981 aaggcctgca agtttccatt cagcctcatc cagtttgcca tcgagtttta tggattgacg
    11041 gatgggtagt gcttttattt cccgctgggt ggaatcggga cgaaaaacag ccgcatcctg
    11101 ggcggatagc cggaacacag atagaataaa gatgaataga aaactgtacc tgatacacat
    11161 aaaggggtac aagataagta aaatgacttg ccgggggata accgtatatc ccaaaattgc
    11221 cgtttttttc tggcaaaagc aggggctgat caccggtaaa taacaaaaag ggcttcccgg
    11281 taagggttgc cctttttatt tgttcgtctt gaagcaattg ttttctatac gcctcaggca
    11341 gccagtggta cattcggaaa actgaatgcg ataacggtct ggtttttctg gtggcgggtg
    11401 atacctacaa aaccaccaat tttttgtgcc tcaaccctga ccaggtcaga taccacattt
    11461 tccgcgccgt agtcagcttc atggttgtcg tatatatgga tcagcaatac ctggctgtat
    11521 tcttttgccg agatccggat acaactacta ctggtttcat tgagtacgtt ctggagtagg
    11581 gaatcgagca cgttggttac cacattccgg tcggcattga gtggtacatt gctgtggact
    11641 tcattgatga aaaagctttg ctttcgcacc acgctttcca gtttaacatt gatcagtgca
    11701 tgtaccagcc gttccagcgt aatcgctttg accggggtgt agttgctcag attctctgct
    11761 gccatgttgt tttttttaag tacgggttgg attccatcgg ggtacggatt agctgatttt
    11821 caaggatcgg attctttctt ggtttttcaa cagctacgga tctaaaacgg gtttttcaag
    11881 gacaataatt ttttcaaagg atacaggtta aaacatggtc ttctcaggat acggtttttt
    11941 caaaggatgc ggatcagccg gcttttcatt tttccgcttc gttcaggcag cggggatatt
    12001 gtttaatttg ctgatacaaa gtaacatcag gtagaagata tacgaagtag actattcaat
    12061 gaatcactag tagtacttca actactgttc cgtaaaatac ctttacccaa aataagtgct
    12121 ttcatgtatt catgactgtg tggatgttgc tagtcagtcc ggctattgat ctgaatcatt
    12181 ggccggttag aatgcagggg cagatgaaaa aaaccacctg gccgccaggt ggtttttttc
    12241 atctttaata aaacggaatt tcattccacg ctattcctct tacgggtaga gcaggtattt
    12301 tttccgcatc tctttgtatt cacttagacc aggttcccaa ctggcgcgga tttccttttc
    12361 agatttacct tccatgatct gttgacggaa ggaggcatgt ccggtgagtt tctcgatcac
    12421 acccatttct ttgctgagtt tgttgtcaaa aaactgttct ttattcgggg aggccttaaa
    12481 cagttcgatc atccattgca ggttgatctg ttttgttttt ctcaactggg atacatcgta
    12541 attccgcagg tcaagtccgt aacaaagctg gttcataaat aagggtgtct ccgacatacc
    12601 tttaatacca gtgggggtga agctgaaatc gtatttgcct ttcaaggacg ggctaccgag
    12661 catggtgaac gggaaataag taccgcgccc atgattgaga taggtgcctt caaataaaca
    12721 ggtagacgga tacagtaaga tggattgctg cgtattcaga ttcggggaag gttttaccgg
    12781 cagggtatag ggcatgtcat gattgtaatt ggccacagga ataatggtaa ccgggcattt
    12841 gactttattg gtcagccagc cttctccatt gaccatccgg gcaaattcac ctacagtcaa
    12901 tccgtgtgca atgggaatcg gaaacatgcc gatgccggat ttgaatttca tatccagtac
    12961 cgggccatct acaaagtatc cgttcggatt gggccggtca aggatcagca tttcttttcc
    13021 gttttcataa caggcatcca tcaggcgtgc gagggcattg atattcgtgt aaaaacgaca
    13081 acccacatcc tggagatcat acaatagaat atctacatcg gcgagatcct gcttcgatgg
    13141 tttgtttttt gatccatata acgatatcac cggaataccg gttaccggat ctgtttcatc
    13201 tgccacatgc accccggcac ttgcattacc ccggaacccg tgttctggcc cgaaaacttt
    13261 tacaatattg accccgcgtt tctgcagact gtctaccagg tgcgtactgc cgatcacggt
    13321 ggttggattg gccatgatgg ccactctttt tccctggagc aggggaatat atttttctgt
    13381 ctggtctgca ccggtcagta tacgaatttc tttttcggtt tgtacctgcc tggggctgcc
    13441 gcaaaaatga aggaagaaag cgaggagggc aagggggatc agtttcatgc ttatgaattg
    13501 taccttaaaa gtaaggaata ttcaaccttg taagtgatcg ttctttccct tttaaaaaat
    13561 gtagtacgta ttaaattcgc cggatatata aaagcgtaaa gggcttactt tccctatgaa
    13621 gtaagccctt taattatcag aagctggtgt catccgcact gggtccgtag ctgccgggca
    13681 acggaatatt taacaaccgg aggtaaacac ccagctgcgc ccggtggtgc acgatctggc
    13741 aaaaacaatg gcgaacattc tctaatctgg aatcggcact gtgaatgata tctccgctgc
    13801 gcatggtcca ggtttcatta accagtttat cgtcggttac cgcagataag gcactcgtac
    13861 cttccgacag tgatttttcg aaatgggcga tcagttcctg ttgattattg atggctgccg
    13921 gtttgtattc cattttggtg aaatccattt cgtccgtgtg caggatcagc ggaatccagc
    13981 ctgttatttc ggccacatgg gttgccagtt gccggatgct catacttttt tcgtggggtt
    14041 tccagtcgta attcccgtcc ggtacccgtt ccagtatttt acgggtggtc cgggcttcat
    14101 tttccatttc tttcaataag gaggcagtaa atgtcatgtt gtttgttttt agaatacaaa
    14161 gcaaacatgc cccactgaca accctatggc agccgcaaaa aaattaataa ttcacaggta
    14221 attgtttcat gtattcattg agctccatat gttctttttt gcggaatggc agaccggtgt
    14281 ccgtgaattt ttttatacgg gaaacccgga agtcccggta atcacgacgg ctatggcacc
    14341 agccgatcag gtgccagttg aaagcgtaaa agataaggcc gatgggttcc actttccgct
    14401 tgctggtttc ttctttttgg ttcaggtatt cgagttcaat gacggagcgg gcagcaatgg
    14461 cattctgaag tgaagggaga tattcaaagt gaagatcata gcaggccggt ttctggaaac
    14521 ggatcttatc attgagtaat tccagttgtt ctttctgatg gccccggaga accgaacgga
    14581 tcttatccag ggcactgccg taatgtttac gcacagactg atcggtgaat ccgttgacca
    14641 gcacttcaat caggagtagg gcattggctt cttcagtact gaaggaaacg ggtgggagaa
    14701 aatagccctg tacaataaaa tagccttttg gcgcttcaaa actgaccggg atgcccagtt
    14761 cgcctaatgc tttcacatcc cggtaaacgg tgcggatact gatggaaaat ttagccgcta
    14821 ttttttccgc ggttacgtat ttacgggatt gcaaaagtgt gagcatgccc agcaggcggt
    14881 ccatccggtt catagcataa atatagcgga aataagcagg cgtccgtcag caaaggtaaa
    14941 acggggatat ggtttaattt ggaaaactgg cttcgagcaa acaaccgtta ccgggtgaag
    15001 aatgtatggc aatattgcca ttatacacct gaagccggct ctgtatattc cggaggccga
    15061 taccggtttt aacttttaac ggatcaaagc ctttgccatc atcctttacc gatatcagta
    15121 cctggttact gctggtgttg tccagtttta tttccaccga ccgggcttcc gcatatttga
    15181 taatattatt gatctgttcc tgtactaccc ggtagatcat cagcttttta tcattttcca
    15241 gttccccttc gttcagggat tggttgtcca gttcgattgt taccggtttc agccggttga
    15301 tattggcgga taatttttca atggattcca ccagtgtttc ctggtcaaag gcaggggtgc
    15361 ggatggtagc ggatagctgc ctgatttcat tgatactttc agcgaccagt tttttaagtt
    15421 gtggtccgag actgggaagg ttttcaggat ggtcggtcac atcaatggca tacatattgg
    15481 ctacggaaag aatctggcaa acgttgtcat gcaattcttc ggcccagcgt gtcctttctg
    15541 tttcctgtgc ctgtataatg gaggcagcca ggg
//
LOCUS       DAOPMW010000037        15439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3805_length_15439_cov_9.526066, whole genome shotgun sequence.
ACCESSION   DAOPMW010000037 DAOPMW010000000
VERSION     DAOPMW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_15439_cov_9.526066"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLU11_03460"
     CDS             complement(<1..303)
                     /locus_tag="PLU11_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58199.1"
                     /translation="MKYSYFLLILFLSNSALLSAQLQVRHIAKNRSIVKKIKESMGKV
                     RFESTNGSVTVQYGVNQGTSDQLTSTSKLLNKAMCLTREEVRGQKSESEMLIADASR"
     gene            complement(340..1692)
                     /locus_tag="PLU11_03465"
     CDS             complement(340..1692)
                     /locus_tag="PLU11_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58200.1"
                     /translation="MKKLLLSVFWISITGILQAQLRIGTGTRIRSAGGTYLVLDSVGI
                     HNESVAIQLEPVVKFTGAGTAAISGSQGITFNSIQVDKTAGGQVRLFQHMNVSQSVSF
                     NNGYIDLNGFETTLDAAATISGENVSAGFTGSGGGFVSITTNLIFPAGANPGNLGAII
                     NAPANLGSTIIRRGHRSQTNGGGGGNSVYRYYDIIPANNTGLNATLRFFYRDEELNGL
                     SESNLQLWKSTDNVNWFLQGFSARDIVANYVTLTGINSFSRWTLSTPGNTLPVSWGSI
                     RLDCALLAPVLWWETLYEQNSMHFEIETSGNGNDWVKAGVINASGNSQDKKSYHFTDL
                     PGRKAAFYRIIQVDADGRRNSSSVIRNTCVSNTALQVWPNPVKKTLTVSVHSAVNGII
                     RLRLSDIQGATILVSQRTVTAGPNLLTVEMEQLPPAIYHLQVFSAGEERPVVIPVVKE
                     "
     gene            complement(1713..2681)
                     /locus_tag="PLU11_03470"
     CDS             complement(1713..2681)
                     /locus_tag="PLU11_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58201.1"
                     /translation="MKKTFLLFALFLPIVTIVAQSVAINTDGAAAHTSAALDIKSTDK
                     GMLVPRMTTAQRTAISNPATGLLIFDTTTGSFWFYNGSSWANLVDSNTGTSVWAANGS
                     DIYNTNAANVGIGTTTPNANALLHLNSTSKGMLLPTLTNDQMTSISSPPVGLMVFNAN
                     TKSPMIYTQRGYQSILPGIFGTQNAWTPISPGPRIIAWGVVDSLLAGGGATPPDNTTH
                     AIGVKSGSNNFSVIWNGPSRWVEITLTNMDYKTDSMILLVTPIGNETWDQAVSTSHTS
                     SGVGTQSRATIKFTDVSRYINGYTGNSIRRRSKFSFILYSLTRDSY"
     gene            complement(2786..3415)
                     /locus_tag="PLU11_03475"
     CDS             complement(2786..3415)
                     /locus_tag="PLU11_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58202.1"
                     /translation="MPVRILIYEDNINLRESLSNLLSLTGDYQVCASFPDCSGVILQV
                     KELMPDVILMDIDMPLVNGIEAVRRIRTFNQAVQIIMLTVFDDNTHVFDAMYAGANGY
                     LLKKYISDKLMHSIREVLDGGAPMSPSIARMVITSMQKAEPARDYQLTPREKEILQFL
                     SRGNSFKMIAIELNISLDTVRTHIKRIYDKLHVCSQIEAVSKAINEKLV"
     gene            complement(3475..6942)
                     /locus_tag="PLU11_03480"
     CDS             complement(3475..6942)
                     /locus_tag="PLU11_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN58203.1"
                     /translation="MKAQLYFKGRTVNAEDGLSDDRVTCFYKDKTGYLWIGTRNGLNR
                     YDGSRFKIFRPGKSNSIASEVIQGITGDKEGKIWVATPSGICIYDPEADSWELIQTPL
                     KKNKNSLPNNMVWDIWFDANGLLWIAVDVFEFVSYDPLKKSYTYYNWPAFARTSPELA
                     GAGNYNSIQRFKAKDDHTFYLGTSKGLVELDTRTGQFRFLGGGYNADVHAIHYDKERG
                     LVYVSVQSGRLFCYRENDNQFSEVQIEPLSYPSRQLIIPDTAEIWLPAETGLLRIFPG
                     AAKAICEMGKLPLDGTLPEGGVNRVFIDDSGIRWVATVNGIFLFDFRILNAAFLPLEE
                     VSPRQGINKMTGLLYEADQDQYFVCLREPATVFLVNRKKKGIQKFTKSADGQLLGPCN
                     RIRKDGAGDIWLMTDLHIYRFDREKEEFILFPSPYKGEWVGYKDMARDSRGNYWFASF
                     RFGLLFYDIRLKAYTEVPGFNQPYRRRVATVLFSDTIHNDLWIGTSGHDLHRYSLKDQ
                     KLYSYGENSGLSELSSLNMINDIRADAEGRIWVATHAGGVFRYLGESKTGAAFTRYSM
                     QEGLPANAVLSLQPVKNGIWFLTGSAISFMSFRDQSEKITTTGQFFSFSSYDSEVGIP
                     HTLTTGSGEEELNTAVSGGLLFYYPQAAYTGPGFPLFVNQILINGKEQNIQPSDMLKL
                     PSGIRSLKLFFAALYYGGRDQLQFQYRLEGYDSSWINGSDRMLAEYQNLPPGNYRFSL
                     RVLNKESKQLTAPAILKIRVQPRFWQTMYFIIPVILLTGYIIFRIIRSLQQKVKEQEQ
                     LNAFATSLYGLAGADDIFWVTTRNCIDILGFEDCVMYQIDEERNCLVQKAAAGPKNPG
                     SGRDVLNPIEIPIGKGIVGAVAATGIAEKIRDTRRDSRYIVDDESRLSEITVPVKIEG
                     KVYAIIDAEHGSRNFFRKRHLRMLEKIAAICAERISKYLTEEKLRAKIARDLHDEMGS
                     TLTSINILSKVGMETGEAGSTVSGYLEKIKDNSAKMMESMSDIVWAINPVNDNLEQLL
                     IRMKEFAAEMLEPARIQYYFETDGELEKSFLNPEQRKDVYLVYKEAVTNAVKYSAATE
                     MTICLMYRQDSLLLQVIDNGKGFDASLEFSGNGLKNMQSRAAGIKAVLSIQSIPGTGT
                     VISLKKNIT"
     gene            complement(7073..8032)
                     /locus_tag="PLU11_03485"
     CDS             complement(7073..8032)
                     /locus_tag="PLU11_03485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58204.1"
                     /translation="MTTAEEEKFIMFRNRLTKVYRHLGKQARRQGVSCYRIYDHDLPE
                     FPLCIEMYGEKLYVAEYKRYHGMTEEEHDSWMEQTMGVAGELLGVEKANIFLKLRQRK
                     PGRLGQYQKYDAVQHEFQVEENGLKFIVNLSDYLDTGLFLDHRLTRQQVRGLCEGKKV
                     LNLFAYTGSFSVYAAAGGASEVVTVDLSKTYLNWSERNLALNGFTDPAIYKTVHADVM
                     QYLKTLPAGYFDLVVMDPPTFSNSKRMEDILDIQRDHAELINDCLAAMNSGGQLFFST
                     NFRKFLLEKEKIHTDHVKDITKATTPFDFEGRLFRYCYHIRKP"
     gene            8217..9602
                     /locus_tag="PLU11_03490"
     CDS             8217..9602
                     /locus_tag="PLU11_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPN58205.1"
                     /translation="MYSIVDIETTGSYAAANGITEISIHLFDGEQVTDHYTTLINPGQ
                     PIPYFIQTMTGITDKMVADAPRFEEVAEKIHSLLKDRIFIAHNVNFDYSFVKSHLQQA
                     GFDLNTKKLCTVRLSRKIFPGLISYSLGKLCTALGITINDRHRATGDSAATVKVFQLL
                     LQNDKDQHIAKSLQRNSKESILPPNVPKTDFEQLPYTPGVYYFHNEKGKVVYVGKANN
                     IRYRVNSHFSNNSQSRQKQHFMQHVHRISYQCCGTELMACILESAEIKKRWPVFNASQ
                     KRWEDVYGIYTYEDQNGYLRLAIDKNRKRLNPVITFHYLVDGHALLRKLIREFTLCPK
                     LCFLQKDNGPCAGPEEKGCGGACEQKESTADYNLRVQHAIRSLMSRPSFAIIDQGLGA
                     EDRSCILVLEGSFYGMGYLPAGFPASEVTLLREQLTQYRENSFIRNLVHGYAARFPEK
                     LIWLSQPVEIK"
     gene            9660..10646
                     /locus_tag="PLU11_03495"
     CDS             9660..10646
                     /locus_tag="PLU11_03495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPN58206.1"
                     /translation="MKNSFSVVFALLFSITLFAQPGTTKPAAKPAPKPSTKAVPKPVQ
                     PVLKNLDDSVGYCVGISVANFYRQMGAKKLNTLLVSRGINDRLGNKQLLIDSITSNTV
                     MNNYLTLLQQAVPKTPVKTVVKPTKALLKNFDDSACYSVGINVANFYKEMGVKKLNTT
                     LVSRGITDLFGKKKSLVDSASINTVMNNYLTLLQQEKSKGNIKAGEDFLAANKNKPGV
                     MVTASGLQYEILRDSAGIKPVAADDVTVHYRGTFIDGSGFDNSYDRGQPITFNLRGVI
                     PGWTEGLQLMSVGSKYKFYVPYTLGYGAFDYMSIPGGSMLIFEVELLAVKKN"
     gene            complement(10760..11833)
                     /locus_tag="PLU11_03500"
     CDS             complement(10760..11833)
                     /locus_tag="PLU11_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HPN58207.1"
                     /translation="MQSIRLKSSTYKLLQPFIVLHIIAVTGILGYMIIEGASFFDSVY
                     MTTITITTVGYGETIPLSTAGRAFTMFLLIASWLTYAFVITRITQFVVSGELNQYFKT
                     RKLMKDISRLNQHVIVCGYGRNGNQAANILKAHHIPFIVVEKDEHLVQRVDEEDTGII
                     RLAGDATDDDVLKAAGVDRARALITTLPIDAQNVFIVLSARSLNPHLKIISRASESTS
                     VAKLKKAGADNVIMPDFIGGTHMATLISKPDVVEFIDFLSGEQGHSIHMESVSFDDLP
                     VNLKDKSLHDVMGWKKTGVNCIGIKDCDGNFLINPPEDTYITPGMRVIVLGTIEQIRE
                     MKGNLGEKREARSEKQEGNSRIS"
     gene            complement(11892..12599)
                     /gene="cmk"
                     /locus_tag="PLU11_03505"
     CDS             complement(11892..12599)
                     /gene="cmk"
                     /locus_tag="PLU11_03505"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPN58208.1"
                     /translation="MSEKKIVITIDGWSSCGKSTLAKRLAKELGYVYVDSGAMYRAVT
                     LYFIRTNVDIDKKKEVAEALTNINLEFVFNSSAQRTEMFLNGENVEFVIRDLLIAEKV
                     SDVAAIREVREFAVAQQQKMGAKKGIVMDGRDIGTVVFPKAELKIFMTADNAVRVERR
                     FKELYAKNPNVTIEEVKNNLEMRDYIDSHREVSPLRKAEDALELDNTSLTEDQQFKKA
                     LDWALARKAGRKISSTH"
     gene            complement(12702..15239)
                     /locus_tag="PLU11_03510"
     CDS             complement(12702..15239)
                     /locus_tag="PLU11_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPN58209.1"
                     /translation="MDNNFSTQVKEIISFSREEALRLGNDFIGTEHLLLGLIREGDNT
                     AVRILKSFNVDLYELRKEIELAVKDKTGKNIANINSLPLTKQAEKVIRVTVLEAKALK
                     SATVETEHLMLSILKNKENIATQILNQFDVDYDLFRQELGIVKSNDPRAEFQDENDDD
                     FDEEKKYSQPKGGSKQAGTAKSKTPVLDNFGRDITKMAELGALDPIVGRESEIERVSQ
                     ILSRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRVLFDKRVISLDLAALVAGTK
                     YRGQFEERMKAIMNELEKNRDVILFIDELHTIVGAGGASGSLDASNIFKPALARGELQ
                     CIGASTLDEYRMYIEKDGALDRRFQKVVIDPPSVDDTIQILNNIKSKYEDYHNVNYDQ
                     EAIDACVKLSDRYVTDRLLPDKAIDVMDEVGARVHLKNINVPQTIVDLEKKIEDVKNE
                     KNKVVKSQKFEEAASLRDTEKRLQEDLEKAKAEWEEESKHKRFPIGEEDIAEVVSMMT
                     GIPVKRMVQAETDKLRKMADDMRGMVIGQDEAILKVVKAIQRNRVGLKDPKKPIGTFI
                     FLGPTGVGKTELARALSRQMFDSEDSLIRIDMSEYMEKFTVSRLIGAPPGYVGYEEGG
                     QLTEKVRRKPYSVILLDEIEKAHPDIYNILLQVLDDGQLTDGLGRKVDFKNTLIIMTS
                     NIGVRQLKDFGAGVGFATASRIEHEDEANKAVIEKALKKTFSPEFLNRIDDVVIFNSL
                     TKENIFNIIDILMKGVTKRLTNLGFSLELTEPAKTFLADKGYDSQFGARPLHRAIQKY
                     LEDPLAEEILDMHVKAGDVLIADVDADNTRLSFTFKSKAGEPSEAQK"
BASE COUNT         4142 a         3889 c         3107 g         4301 t
ORIGIN      
        1 acgtgaagcg tccgcaatta acatttcaga ctcacttttc tgacccctta cttcttccct
       61 tgtcagacac atggctttat ttaacaattt tgatgtgctg gttagctgat cagaggtacc
      121 ctggtttaca ccatactgaa ccgttacaga accattcgtt gactcaaaac gaaccttccc
      181 catcgactct tttatctttt ttactatcga cctgtttttg gcaatatgcc gaacctgcaa
      241 ctgggcagag agcaaagccg aattacttaa aaaaagaatc agtaaaaaat agctgtattt
      301 cataaaatag gttttttaaa aacacccgtt aaattatttc tattccttta caaccggaat
      361 cactacgggc cgctcttcac ctgctgaaaa tacctgcagg tgataaatgg caggtggtag
      421 ttgttccatt tccactgtaa gcagattggg gcctgcagtt actgttcgtt gagaaaccag
      481 aatggttgca ccctgaatat ctgacaaccg tagccggatt attccgttta cagcactatg
      541 cacggatacc gtaagcgttt ttttaactgg gtttggccag acctgtaaag ccgtattact
      601 gacacaggta ttccggataa cagatgagct gtttcgccgg ccgtcagcat ctacctgaat
      661 aatccggtaa aaggccgctt ttctcccagg cagatctgta aaatgataac tttttttatc
      721 ctgactattt ccagacgcat tgataacacc ggccttcacc cagtcattcc cattcccgct
      781 cgtctcaatc tcaaaatgca tactattctg ttcatataaa gtctcccacc aaagtactgg
      841 tgccaacagg gcacagtcca gccggatact tccccaactt acaggcaggg tattgccagg
      901 agtggaaagt gtccagcggg agaaactgtt tataccggtg agcgttacat agttggccac
      961 aatatcacgt gcactgaatc cctgcaagaa ccagtttaca ttgtctgtac tcttccataa
     1021 ttgcaggtta ctttcagaaa ggccgttcag ttcctcatcc cggtaaaaaa aacgaagcgt
     1081 agcattgagc cctgtattat tcgccggtat tatatcataa taacgataaa ctgaattacc
     1141 tccgccgcca ccgtttgtct gtgatcggtg tcctcttcgg ataatggtgc ttcccaggtt
     1201 agccggcgca ttgataatgg cccccaggtt tccgggattc gcccccgccg ggaaaattaa
     1261 attagtggtg attgaaacaa atcctccccc ggatccggta aaacctgcac tcacattttc
     1321 gccactgatc gtagcggcag catccaacgt tgtttcaaat ccattcaggt caatatagcc
     1381 attattaaaa ctgacgctct gtgacacatt catgtgttga aataaccgga cctgtccacc
     1441 agcagtttta tcaacctgga ttgaattgaa tgttatgccc tgagaaccgc tgatagcagc
     1501 ggttccggct ccggtaaatt tcacaacagg ttccagttgg attgccacac tttcattatg
     1561 tatacccaca ctatccaata ccaggtaagt acccccggca gaccggatcc gggtaccggt
     1621 cccaatcctc aactgagcct gcaaaatccc cgttattgag atccagaaaa cagataaaag
     1681 caattttttc attaaaagtt gtttcacttt atttaataag aatctcttgt taaggaatag
     1741 agaatgaatg aaaacttact cctccggcga atcgagttcc cggtataacc atttatatat
     1801 ctggaaacat ctgtaaactt gatggttgca cgactttgtg tccctacacc tgagctagta
     1861 tgacttgtag acacagcctg atcccatgtt tcattaccaa tcggggtcac taagagaatc
     1921 atactgtctg ttttatagtc catgttagtc aacgtgattt caacccacct gctagggcca
     1981 ttccatatta cagaaaaatt attactgccc gatttgactc caattgcgtg tgtggtatta
     2041 tctggtggag tcgctccacc tccggcaaga agtgaatcaa caactcccca ggcaataatt
     2101 cttggcccgg gtgaaattgg ggtccaggca ttctgcgttc caaatattcc cggaagaatg
     2161 ctctgatatc cacgttgcgt ataaatcatg ggactcttcg tattagcgtt aaataccatt
     2221 aaaccaaccg ggggactact gattgaagtc atctgatcgt tggtaagtgt tggcaataac
     2281 attccttttg aagttgagtt aagatgtaat aaggcattgg cattcggagt tgtagtccca
     2341 atgcccacat tagcggcatt ggtattataa atatcactgc cattggccgc ccatacagag
     2401 gttccggtat tactatccac caggttggcc caggaactgc cattataaaa ccagaaactg
     2461 cctgtagtag tatcaaatat caacaacccg gtggccgggt tggaaatagc agtccgctgg
     2521 gcggtagtca ttctgggcac cagcatcccc ttatcagttg atttgatatc caatgcggca
     2581 ctcgtgtgtg cagcagctcc atccgtattg atggcaacac tttgggcaac tatcgtcaca
     2641 atcggtaaaa aaagggcaaa aaggaggaat gtctttttca tttttccggc atttttattt
     2701 actgtaaaaa tggagcggaa aggaatacaa gacaataccg caatgatgtg tcacctgatc
     2761 agggtacagg aaggcagaaa cgtaatcaaa cgagtttttc gttgatggct ttgctcaccg
     2821 cttcaatttg cgagcagaca tgaagtttgt cgtaaatgcg tttaatatgc gtccggactg
     2881 tatcgaggct gatattcagt tcgatggcaa tcattttaaa gctgttccct cttgagagaa
     2941 actgcagaat ttccttctct cttggtgtaa gctgataatc cctggccggt tccgcttttt
     3001 gcatgctggt gattaccata cgggcaatgc tcggactcat tggagcacct ccatccagta
     3061 cttcacgtat ggaatgcatg agcttatctg atatatattt tttcagtaaa tatccattgg
     3121 cgccggcata catggcatca aaaacatggg tattatcatc gaaaaccgtc agcatgataa
     3181 tctgaacagc ctggttaaat gtacggattc tcctgactgc ttcaatcccg tttacgaggg
     3241 gcatatcgat atccatcagg atcacatccg gcatcagctc ctttacctgc agaataaccc
     3301 cggaacagtc cgggaaactg gcacatacct gataatcccc tgttaaagaa agcagattac
     3361 tcaggctctc ccgcagatta atattgtctt cgtatataag gattctgacc ggcatgttaa
     3421 ataaaactat gggctgcaac ttaatgcagt tccctaaatt aatctatacc ctaatcaggt
     3481 gatatttttt ttcaacgaaa tcaccgtacc tgtaccggga atggactgga ttgataatac
     3541 agcctttata ccggctgccc ggctttgcat atttttcaga ccatttccac tgaattcaag
     3601 actggcatca aaccccttcc cattgtcaat tacctgcaag agcagactat cctgtctgta
     3661 cattaaacaa atcgtcattt ccgtagcagc actgtacttc accgcatttg tcacggcttc
     3721 tttatagacc aggtatacat ctttccgctg ttccggattc aggaatgatt tttccagctc
     3781 cccatccgtt tcaaaataat actgaatcct ggctggttca agcatttccg ccgcaaattc
     3841 cttcatccgg ataagtaatt gttccaggtt gtcattcacc ggattgatgg cccagacgat
     3901 atcactcatg ctttccatca tttttgctga attgtcttta attttttcaa ggtaaccact
     3961 cacagtactg ccggcctccc cggtctccat tcccacttta ctgaggatat taatactcgt
     4021 aagggttgag cccatttcat catgcagatc acgggcaatt tttgcccgta atttttcttc
     4081 agtcaggtat ttactgatcc gttccgcgca tatcgccgcg attttttcca gcattcttaa
     4141 atgtcttttc ctgaaaaaat ttctactgcc atgttctgca tcaataatgg catacacttt
     4201 accttcaatt ttaacgggta cggtgatctc agataacctg gactcatcgt ccacaatata
     4261 cctgctatca cgtctggtgt ccctgatttt ttccgcaata cctgttgccg ctacagcgcc
     4321 tacaatcccc tttcctatgg gaatttcaat cggattcagc acgtccctcc cgctgccggg
     4381 gtttttggga cctgcagccg ctttctgtac caaacagttc ctttcttcat cgatctggta
     4441 cattacacaa tcttcaaagc cgagaatatc tatgcaattt ctggtagtca cccagaaaat
     4501 atcatctgca cccgcaagtc catacaatga agtggcaaat gcattcagtt gctcctgttc
     4561 ctttactttt tgctgcagtg aacgaataat ccggaaaatt atgtatcctg taagcagaat
     4621 aacaggtata ataaaataca tggtctgcca gaaccggggt tgcaccctga ttttcaatat
     4681 ggccggagcc gtcagttgct tgctttcttt attcaatacc cgaaggctga accggtaatt
     4741 accaggaggc aggttctgat actctgccaa catacgatct gaaccattta tccagctgct
     4801 gtcatacccc tcgagcctgt attgaaactg cagctgatca cggcctccat aatataaagc
     4861 ggcaaaaaat aacttcagcg atctgatacc agaaggcaat ttcaacatat cacttggttg
     4921 tatattctgc tctttcccgt ttatcagaat ctggtttacg aataacggga atcctggacc
     4981 tgtatacgct gcctgcggat aataaaacaa aagccccccc gaaactgctg tattcagttc
     5041 ctcttctccg ctgcctgttg taagggtatg gggtatacct acctcagaat cataagaact
     5101 gaacgaaaaa aactgacccg ttgttgtgat tttttcagac tgatccctga atgacataaa
     5161 tgaaatcgca cttccggtca aaaaccaaat accatttttt acaggttgca aggataatac
     5221 agcattggcc ggcaatcctt cctgcatgga ataccgggta aatgctgcac ctgttttact
     5281 ttctcctaaa tacctgaaaa cgcctccggc atgagtggct acccatattc tgccctcggc
     5341 atccgcacgg atatcattaa tcatattcaa cgaggacaac tcagaaagac cactattttc
     5401 accataactg tatagctttt ggtctttcag tgaatagcgg tgcagatcat gaccagatgt
     5461 acctatccat agatcgttat gtattgtatc agaaaacaat acagtagcca cccgccgccg
     5521 gtaaggttga ttaaatcccg gcacctcagt ataagccttt aacctgatgt cgtaaaataa
     5581 caaaccaaac cgaaaggagg caaaccagta attaccacgg gaatctctgg ccatatcttt
     5641 gtagccaacc cattccccct tataagggct ggggaaaaga ataaattctt ctttttcccg
     5701 gtcaaaccga tagatgtgta aatctgtcat cagccagata tccccggctc catctttcct
     5761 gatacggttg catggtccca gaagctgacc atctgctgat tttgtaaatt tctgtatccc
     5821 cttttttttg cgattgacca gaaatacagt tgcaggctca cgtagacaaa caaaatactg
     5881 gtcctgatcc gcttcataaa gcagacccgt cattttattt ataccctggc ggggagaaac
     5941 ctcctctaac ggaagaaatg cggcgttcag aatacggaag tcaaacaaaa aaatgccatt
     6001 cactgttgcc acccagcgaa tcccgctatc atcaataaat acccggttca ccccaccctc
     6061 tgggagtgtc ccatcaagcg ggagtttccc catctcacaa attgcttttg ccgcacctgg
     6121 aaaaattctg agcaaaccgg tctctgcagg cagccagatt tcagcggtat cagggataat
     6181 caattgccgg gatggatagg aaagcggttc aatctgaact tcactgaatt gattgtcatt
     6241 ttcccggtaa caaaataaac ggccagactg aactgataca taaactaaac cccgctcctt
     6301 gtcataatgt attgcgtgca catccgcatt atatcccccg cccagaaacc gaaactgccc
     6361 ggtccgtgta tccagttcaa caagcccttt actcgttcca agataaaatg tatgatcatc
     6421 ctttgcctta aaacgttgaa ttgaattata atttccggct ccggccagtt ctggtgatgt
     6481 ccgggcaaac gccggccagt tataatatgt atatgacttc tttaggggat cgtaagaaac
     6541 aaattcaaaa acatccaccg ctatccataa aagtccattt gcgtcaaacc agatatccca
     6601 aaccatgtta ttggggaggc tgttcttgtt ttttttgagt ggcgtttgaa tcaattccca
     6661 actgtcagcc tcaggatcat aaatacagat acctgagggt gttgccaccc agattttccc
     6721 ctccttgtca cccgttattc cctgaatcac ttccgaggct atggagttac tttttccagg
     6781 tctgaaaatt ttgaaccggc ttccatcata gcggttaagc ccattacggg tgcctatcca
     6841 gagatatccg gttttatcct tataaaaaca agtaacccgg tcgtcagata agccgtcttc
     6901 ggcattaacc gtacgccctt tgaaataaag ctgcgccttc atttcaactg gaaataaagg
     6961 aaaacaaaaa acaatcgtga tcagggaaga aaataatgtc cgcctcaggt taaagtattt
     7021 atagcacaaa gtagaaaaaa aatgctggaa tactataccc tgatcaggtg agttacggct
     7081 ttcggatatg ataacaatac cgaaacaacc tcccttcaaa atcaaagggt gtcgtggctt
     7141 ttgtaatatc ttttacatgg tccgtatgga ttttctcttt ttccagaaga aacttccgga
     7201 aattagtact gaaaaataac tgccctccgg aattcatcgc cgccaggcaa tcattgatca
     7261 actccgcatg atcccgctgg atatcgagaa tatcctccat ccgtttgctg ttactgaaag
     7321 taggcgggtc cataacaacc aggtcaaaat atccagccgg taatgtcttc aggtactgca
     7381 tcacatccgc atgtaccgtt ttgtatattg ccggatccgt aaacccattc agtgccaggt
     7441 tgcgctcgga ccagttgagg tatgttttgg ataaatcaac agtcaccacc tcactggccc
     7501 cgccagcagc cgcataaacg gagaaagaac cagtataggc aaagaggttc agtaccttct
     7561 tcccttcaca caatcccctt acctgttgcc gggtgaggcg gtgatcgagg aataatccgg
     7621 tatccagata gtcactcagg ttcacaataa atttcaatcc gttctcctcc acctgaaatt
     7681 catgttgcac cgcatcatac ttctggtatt gccctaaccg gccgggtttg cgctgacgca
     7741 gtttaagaaa aatattggct ttttccaccc ccagtaactc cccggccaca cccattgtct
     7801 gttccatcca gctgtcatgc tcctcttccg tcatcccatg atagcgtttg tactcggcca
     7861 catataattt ttcaccatac atctcaatac aaagaggaaa ttccggaaga tcatgatcat
     7921 agatccggta acaggatact ccctgcctcc tggcctgttt gcccagatgc cggtaaacct
     7981 tggtcagccg gttccggaac attataaact tttcttcttc tgcggtagtc atacttattt
     8041 tcacgaaatt tatgcttcaa agatcgtttt tatagttgat aagttgaaag gtttacaagt
     8101 taacaaggcc ggggtttacc ttgtcaacct ttcaacttat caacccgtca acctgtttgt
     8161 aaccctgtca actttttacc ctatcaaccc gtcaacctgt aaacttatca actcccttgt
     8221 atagcatcgt cgacatagaa accaccggca gttatgcggc cgccaacggg atcaccgaga
     8281 tctcgatcca tctctttgac ggggagcagg tgacggatca ttataccaca ttgatcaatc
     8341 cgggacaacc tattccttat tttatccaga caatgaccgg cattacggat aaaatggtgg
     8401 ctgatgcacc ccgatttgag gaggtcgctg aaaaaatcca tagcctgctt aaagaccgta
     8461 ttttcatagc acataatgta aactttgatt actcttttgt caaatcgcat ctgcaacaag
     8521 ccggttttga cctgaataca aagaagcttt gtacagtacg attgagccgg aaaatttttc
     8581 ccggcctcat ttcctacagc ctgggcaaac tttgtaccgc actcggaata accattaatg
     8641 accggcaccg ggcaacgggc gatagtgcag ccaccgttaa agtctttcag ttgttgttgc
     8701 aaaacgacaa ggatcagcat attgccaaaa gcctgcaacg gaattcaaag gaatcaattc
     8761 ttccgccgaa tgttcctaaa accgactttg aacaattgcc ctatactccc ggggtatact
     8821 atttccataa tgaaaaaggg aaagtggtct atgtgggcaa ggcgaataat atccgttacc
     8881 gggtcaacag tcatttcagt aataactcac agagccgcca gaaacaacat ttcatgcagc
     8941 atgtacaccg gatcagttac caatgctgcg gcaccgagct gatggcctgt attctcgaat
     9001 cggccgagat caaaaaaaga tggcccgttt tcaacgcttc acaaaaaaga tgggaagatg
     9061 tgtatggcat ttatacctat gaagaccaga acggttactt acggctggcc attgataaaa
     9121 acaggaaacg gttaaacccg gtcatcacct tccattacct ggtagatgga catgccttgc
     9181 ttcgtaaact gatccgggaa tttacccttt gtcctaagct ttgtttcctg cagaaagata
     9241 atggcccatg tgccggcccg gaagaaaaag gatgcggggg cgcctgtgaa caaaaagaaa
     9301 gtacagctga ttataacctg cgggtgcaac atgccatccg ttcattaatg tcccggccat
     9361 cctttgccat cattgaccag ggattaggag cggaagaccg ttcctgcatc ctggtgctgg
     9421 aaggcagttt ttatggcatg ggctatcttc cagctggatt cccggcttcc gaggttacct
     9481 tactacggga acaactgaca caataccgcg aaaacagctt tatccgtaac ctggtgcatg
     9541 gatatgccgc ccgtttcccg gaaaaactga tctggcttag ccagcccgtt gaaattaaat
     9601 aagcccggat tcccgttttc cccggccaaa ctgtattttt ggcccctaat aaaatgtaca
     9661 tgaaaaacag cttttccgta gtctttgccc tacttttttc cattacgctc ttcgctcagc
     9721 cgggtacgac caagccggca gcaaaacccg ctccgaaacc cagcaccaaa gccgtcccca
     9781 aaccagtgca gcctgtttta aaaaacctgg atgattccgt tggctactgt gtcggcatca
     9841 gtgtagctaa tttttaccgg caaatggggg ccaaaaaatt aaacaccctg cttgtatccc
     9901 gtggcatcaa tgaccgcctg ggcaataaac aactgctgat tgattccatt accagcaata
     9961 cggtcatgaa taattacctt accctcttac aacaggcagt tcccaaaaca cccgtaaaga
    10021 ctgttgtaaa accaacaaaa gccctgctaa aaaactttga tgactctgcc tgttattccg
    10081 ttggtattaa tgtggctaat ttttataaag aaatgggggt gaaaaaatta aacaccaccc
    10141 tggtttcccg tggaattacc gacttatttg gaaaaaagaa atccctggtt gattcggctt
    10201 ccattaacac agtcatgaat aactacctga ccctgttgca acaggaaaaa tcaaaaggca
    10261 atataaaagc gggtgaggat tttctggcag caaataaaaa taaaccaggg gtaatggtta
    10321 cagccagcgg actgcaatat gaaatactga gagacagtgc aggcattaaa ccagtcgccg
    10381 ctgatgatgt gaccgtacat tacaggggca catttataga tggttccgga tttgacaact
    10441 cttatgaccg tggacaaccc atcacattca atcttcgtgg ggtaatcccc ggctggaccg
    10501 aaggattgca gttaatgtcc gttggttcca aatacaaatt ttatgtaccg tatacactgg
    10561 gctacggagc atttgactat atgtccattc ctggtggctc catgctcatc tttgaagtgg
    10621 aactgcttgc tgtgaagaaa aactaagaac tataaataat aaaaagcggg tattgacaaa
    10681 atcaataccc gcttttttgt tataaccaca gagaacgcgg agaaatacaa aaagctgcat
    10741 aaagtgaata cttctcgctt tacgaaatgc ggctgttccc ttcttgcttc tcgcttcttg
    10801 cttctcgctt ttctccgagg ttccccttca tctccctgat ctgctcaatc gtacccaaca
    10861 caattacccg catacccggt gtgatatacg tatcttctgg tggattaatc aggaaattac
    10921 catcacaatc tttgatcccg atacaattga ctccggtctt tttccatccc atcacatcat
    10981 gcagtgattt gtctttaaga ttgaccggca ggtcatcaaa acttaccgat tccatatgaa
    11041 tggaatggcc ctgttcgccc gaaagaaaat caataaactc gaccacatcc ggttttgaga
    11101 tcagggtggc catatgcgta ccgccgataa agtcaggcat gatcacattg tctgccccgg
    11161 ctttttttaa tttggcaacc gaggtagatt cagaagcccg gctgataatt ttcaaatggg
    11221 gatttagtga acgagccgac aatacaataa atacattctg tgcatcaatg ggtaaagtcg
    11281 taatcagtgc ccgggccctg tcgacccccg cagctttcag tacatcgtca tcggttgcgt
    11341 cacctgccag ccggatgatt ccggtatctt cttcatccac ccgttgtacc aggtgttcat
    11401 ctttttccac cacaataaag ggaatgtgat gtgctttgag aatattggcc gcctggtttc
    11461 cattccggcc atatccacag acaatcacat gttgatttaa cctggaaata tctttcataa
    11521 gtttccttgt tttaaaatac tgatttaatt ccccgctcac gacaaactgg gtgatccggg
    11581 taatcacaaa tgcataggtg agccagctgg cgatcagcag gaacatggta aaggcacggc
    11641 cggctgttga cagaggaatg gtttcgccat acccgacagt ggtaatggtg atagtggtca
    11701 tatagaccga atcgaagaaa gaggcccctt caataatcat gtagcccagg ataccggtca
    11761 ccgcaataat atgaaggaca ataaatggct gtagcagttt gtacgtggaa ctcttcaatc
    11821 gtatgctttg catcaaatat aaaagggttc aggggttgaa cagctgaccg atatcagcca
    11881 agatactgaa attaatgggt actgctgatc ttccggccgg ctttacgggc caatgcccag
    11941 tccagggctt ttttaaactg ctggtcttcg gtgaggctgg tattatccag ttccagggca
    12001 tcttccgctt tccgcaacgg actcacttcc cggtgcgaat caatataatc ccgcatctcc
    12061 aaattatttt tcacttcctc aatggttaca ttggggttct tggcatataa ctctttgaac
    12121 cggcgttcga cccgtactgc attatccgca gtcataaata ttttcagttc ggcttttgga
    12181 aaaaccaccg tgccgatatc ccggccatcc attacaatgc ctttctttgc ccccatcttc
    12241 tgctgctggg ccacggcaaa ctcacgcact tcacggatgg cagccacatc actgactttc
    12301 tcagcgatga gcaggtcacg gatgacaaac tccacattct ccccgttgag aaacatttct
    12361 gtacgctgcg cggaggaatt aaaaacaaat tcaaggttaa tatttgtaag cgcttccgct
    12421 acttccttct tcttatcgat gtccacattg gtccggataa agtacagcgt caccgcccgg
    12481 tacatggccc cgctgtccac atacacataa ccgagctctt tagccagtcg cttggccagg
    12541 gtgctcttgc cgcaactgga ccagccatca atggttataa cgattttttt ttcagacata
    12601 ctccgggcaa atgtaaataa aaaagggggt gtcttaaaag tttgtctcct gccaaatagc
    12661 aggataaaac gtctaagaca cccctatcaa aaaaaactac tttacttctg ggcttcagag
    12721 ggctccccgg ccttgctttt aaaggtaaac gacaaacggg tattgtccgc atccacatct
    12781 gcaatcagta catcacctgc tttcacatgc atatccaata tctcttctgc caggggatct
    12841 tccaggtatt tctggatggc cctgtgcagc ggacgggcgc cgaactgaga gtcataccct
    12901 ttatccgcaa ggaaggtctt ggccggctcg gtaagctcaa ggctgaatcc gagattcgta
    12961 agacgcttgg ttaccccctt catcaggata tcaataatgt tgaagatatt ctctttggtc
    13021 aggctgttaa agatcaccac atcatcaatc cggttcagga actcaggaga gaaggttttc
    13081 tttagtgctt tttcaatcac cgctttatta gcttcatctt catgctcaat tcttgaagcc
    13141 gtggcaaatc ccacaccggc gccaaaatct ttcagctgac gtaccccgat attggatgtc
    13201 atgatgatca gggtattttt gaaatccact ttgcgtccga gtccatcggt taactgaccg
    13261 tcatccagca cctgtaaaag aatattataa atatccggat gcgctttctc aatctcatcc
    13321 agcaggatca cactatacgg cttacgacgt actttctccg tcagctgtcc gccttcttca
    13381 tagcctacat agccgggagg ggcaccaatc aggcggctca cggtgaattt ctccatgtac
    13441 tcactcatat cgatacggat cagtgaatcc tccgaatcaa acatctggcg ggagagcgca
    13501 cgagccagtt cggttttacc caccccggta ggaccgagga agataaaagt gccaatcggc
    13561 tttttgggat ccttcaaacc aacgcggtta cgctgaatcg ctttcaccac tttcagaatg
    13621 gcttcgtcct gaccgattac cattccacgc atatcatcag ccatcttacg gagcttgtcg
    13681 gtttctgcct gcaccatccg cttcacgggg atgccggtca tcatactcac tacttccgca
    13741 atatcctctt ccccgattgg gaaacgttta tgcttgcttt cctcttccca ttctgccttc
    13801 gctttctcca ggtcttcctg cagtcttttt tcagtatccc gtaaagaagc ggcttcttca
    13861 aacttctggc tcttcaccac tttattcttc tcgttcttta catcttctat ctttttctcc
    13921 aggtccacga tggtctgggg cacattgata tttttcaggt gcacccgggc gcctacttca
    13981 tccatcacgt caatggcttt atcaggcagt aaacgatcgg ttacataacg gtcactcaat
    14041 ttcacacagg catcaatcgc ttcctggtca tagttcacgt tatggtaatc ctcgtatttg
    14101 gacttgatat tattcagtat ctggatggta tcatccacac tgggcggatc gatcaccact
    14161 ttctggaaac gacggtcaag ggcgccatct ttctcaatgt acatccggta ttcatccagg
    14221 gtggaagcgc caatacattg gagctcgcca cgggccaatg ctggtttaaa gatattgctg
    14281 gcatccagag aaccactggc accacccgca cccacgatgg tatgcagctc atcgatgaac
    14341 agaatcacat cccggttttt ctccagctca ttcataatgg ccttcatccg ctcctcaaac
    14401 tggccacggt acttggtacc agccaccagg gccgccagat caagggagat cacccgctta
    14461 tcaaacaata cacgggaaac tttacgttgt acaatgcgga gggccaggcc ttccacaata
    14521 gcggtcttac ccactcccgg ttccccgatc aggatcgggt tattcttttt ccggcgggaa
    14581 aggatctgcg atacccgttc gatctcggac tcacgtccaa caatcggatc aagggccccg
    14641 agttccgcca tcttagtaat gtcacggcca aaattgtcta ataccggtgt tttgcttttg
    14701 gcagtaccgg cttgtttact gccgcctttc ggctgagaat attttttctc ttcatcaaaa
    14761 tcatcatcat tctcatcctg aaattcggcc cggggatcgt ttgattttac gatgcccagc
    14821 tcttgtctga aaagatcgta gtccacgtca aactgattta aaatttgagt tgcaatattt
    14881 tctttgttct taagaataga cagcatcaga tgttcggtct ccactgtcgc gcttttcagg
    14941 gccttggcct ccagtacggt cacgcggatc actttttctg cctgtttggt caggggcaga
    15001 ctgttgatat tcgcaatgtt ctttcccgtc ttatccttta ccgccagttc gatctctttc
    15061 cgcaattcat aaaggtccac attgaagctt ttcaggatcc ggactgctgt attgtctcct
    15121 tcgcggatca gccccagcaa aaggtgctcc gttcctataa aatcattgcc cagccgtagt
    15181 gcttcctccc tgctgaacga aataatctcc ttaacctgtg ttgaaaaatt attatccatt
    15241 ttaagataat tggttgtgat acaatattaa cgaataaatt tggttctttt tccggggtac
    15301 ttataaacgg tctgttgata atttaagttg caggagaaac cctgattttt actttgcaga
    15361 cgggatagaa gtgccaatta ctaaacgtaa gaactatgaa tgtcaaaacc gataccaaag
    15421 agaaatttca tgccatcac
//
LOCUS       DAOPMW010000038        15302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3872_length_15302_cov_8.397980, whole genome shotgun sequence.
ACCESSION   DAOPMW010000038 DAOPMW010000000
VERSION     DAOPMW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3872_length_15302_cov_8.397980"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /gene="asnB"
                     /locus_tag="PLU11_03515"
     CDS             <1..1578
                     /gene="asnB"
                     /locus_tag="PLU11_03515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN58210.1"
                     /translation="EVVLKCYQEYGIGSVEKFIGMFAYAIYDQPKQELYILRDRAGVK
                     PLYYYHANNTLLFASELKSFHAYPLFEKKIEPQAVSLFFKYGYIRDPFTIFQNTHKVR
                     PGHYIKIDIRTKTVTDHKYWDVVEHYNKPKIQISEEEATAELEKLFRSAFEYRMVSDV
                     PVGVFLSGGYDSTLVTAILQANSAQKIKTFTIGFHEKKWNEAPYAKEIAAHLGTDHHE
                     YYCTTKEAQDIFPQLADIYDEPFGDSSGIPTTLVSRFARKQVTVSLSADGGDEIFAGY
                     NKYQQMQKLNNYLLSAPGPLLALGKGAANLSMKAIPGLRRFHKVERLTEILNTYRNAG
                     LSDIYSRQYKNNDLYDLLPGYNDKLGLFDEMEILNSGNDFINKSLAYDYRTYMVDDIL
                     TKVDRATMSVALEGREPLLDHRIIEFASQLPSNLKYHNGEKKYLVKKIVHKYIPKEVM
                     DRPKTGFSIPVFDWLTGDLKDHLYSLINEKEFAKHGLIDIKKAMSVRKEFEDGRKGRD
                     AQIWLLLMFQMWWNRWM"
     gene            1624..2928
                     /locus_tag="PLU11_03520"
     CDS             1624..2928
                     /locus_tag="PLU11_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN58211.1"
                     /translation="MKILIVNYRFFVSGGPERYLFNIMALLKKKGHTVIPFSVSNQHN
                     QPSDYRDYFMSPVGEGKEVYFNEYSKRNLKTLWKTFSRMFYSVEARRQLSRLIKDTQP
                     DLVYVLHYQNKMSASVFDAAARLKVPVVHRVSDFSQICANGLFYRPQQKDVCERCLHG
                     TRLNAVKHKCVHDSYFLSAVKAASLKLQQFLHLDKKIAAYVVPSGFTISKLAVFGLPP
                     AKLRHIPTFFNFSIMKENLAISYGSYALFVGRVEEEKGLYTMVNAFIDTPYSLKIIGG
                     SSSGFDAKLKSYLAGKNHHIELLGQLAFSEIQQYLAACAFTVMPTEVYDNFPNTVLES
                     FAFRKAVVATNVGSLREIVNDQENGLLFTLKDAVSLRNCVQSLFEDSDKCRRLGEQAF
                     RKLNDEYSEEQHYTKLMHCFETVVDEVRQKPVLVQAAKTFVN"
     gene            2934..3950
                     /locus_tag="PLU11_03525"
     CDS             2934..3950
                     /locus_tag="PLU11_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58212.1"
                     /translation="MRIIILADFKVKAGSDFWIREELEKLGYTTELLGIQNYNPKDDT
                     TRFGKIKIWYKYFKLAGKGLSVSGKEDVLISDNFVIGAITAFFGRLSGRKRKVIALNM
                     IAHEKGLFNRIFRKIVYNTAFRNRGFWFSVNDSQLIDLYAQQFRFPEDRVFVLQDPFF
                     KSDEQADFTGEGDFVFTGGDAYRDWDAVIRCAKELPQIRFTGVARKKYFPGHTELPIN
                     LKMYFDTTQDEFYGLMKNSRLVFLPLNSKAPCGLIVMMRAALLSKPVVISETPSTQNY
                     IRNKISGILLPLQDQLGMKKEISELFWSQELQQKYAAELKHFILENFSTGSTMKKIEE
                     IIKK"
     gene            3982..4884
                     /locus_tag="PLU11_03530"
     CDS             3982..4884
                     /locus_tag="PLU11_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN58213.1"
                     /translation="MKKRIYMAGCGGMLGEAFYTQFKDDYEIKCTDKDVNVSWLSFLD
                     FRDFDAYKKDVLDFKPDYLFHLGAYTDLEFCEQNAEDTYKTNTLAVENAVYLANQLDI
                     PLLYISTAGIFDGKKELYDDWDQPNPLGVYARSKYMGERYVVENARRFLVCRAGWMMG
                     AGPSKDKKFVQKLMKQLKEGKKELFIVDDKDGTPTFTHDFARNVKALIQKEYWGLYNM
                     VCGGQTSRLEVAGELLKLMGLEKEVKITSVNSDYFKQTYFAERPPCERLVNRKLELRE
                     LNLMQDWKSALHQYVNAYYKDYLK"
     gene            4887..6011
                     /locus_tag="PLU11_03535"
     CDS             4887..6011
                     /locus_tag="PLU11_03535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN58214.1"
                     /translation="MKKLRIAAFGFRTLPPAKGAAGADKFAMELFPRLVQRGHTVTAY
                     NRRYPDVHQDITEYKGVRIKTFKTVNRKGFDTLLHSLKCTCHIIFRNTADVVHIQNGG
                     NSIWALPLRLFGKKVFISQDGVDWKRDKWPWYGKLYLRISTYISAYFPSDIIFDNVIA
                     RRYFEERFKKKFAFIPFGSEVAPVVPGEEVFTRFGLEKDSYYLFVGRFIPDKGLHYLI
                     PAFEKSGSRRKLVMIGGSPNPSSYENELYAMASDKVVFTGFLYGDDVTRLMKNCYCYI
                     QPSDVEGLSPVILSVMGLGVPLIVSDIEENIYAVQDTAITFSKADTNSLTEKIRYAEQ
                     HHPHMKELAVKAQNRALTEFNWDNVAEQHEQIFSGIKQVR"
     gene            6035..7414
                     /locus_tag="PLU11_03540"
     CDS             6035..7414
                     /locus_tag="PLU11_03540"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HPN58215.1"
                     /translation="MTNKNKGIFQLFFAGMDLFALNAVHLALIMVMNRIDRSGQQYNA
                     FFIVSNMAWLASAYINAVYARTNRFNFDYFGKQSLKAYLLFVIFILFFIFIYNYSYSR
                     LFIISNFLGFGLIILFTRLLFLLLSVYMRKGNRFKKKVVVLGYNDLSKRLVGHFMVSD
                     QLSMEGYFEDPQEVHELSTFPILGNRKECVSYAIENEVDEIYSTVSPESNKYVYDMAQ
                     VAESNMIRFKFVPDFQLFVNRNTHIDFINDIPILSLRSEPLDDITARIKKRVFDIVFS
                     TMVAVLLLSWLVPLMAILIKLSSRGPVFFVQLRSGRDNKSFKCFKFRTLRVNREADTK
                     QVTQNDNRITRLGAFLRKSNIDELPQFFNVIIGNMSVVGPRPHMLRHTEDYSRVLNEY
                     MIRHFVKPGVTGWAQVNGFRGEIREKEQLRKRIEYDIWYLENWNLWFDLRVIFLTVWV
                     TIKGDKNAY"
     gene            7447..8937
                     /locus_tag="PLU11_03545"
     CDS             7447..8937
                     /locus_tag="PLU11_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HPN58216.1"
                     /translation="MFIEKIINALTRQISHRPDHMAFCIAGEHITYAGFGNQIATIRQ
                     ALRTIDNKNHNVGLVTNDDIQTYASIFALWMEGHAYVPLHHTQPAERSHEIIGQAEIS
                     LVLNSLPGGSFQGARLLITKDLPAASVLHEPVVVPDSALAYILFTSGSTGIPKGVPIT
                     RSNLVVFMDAFMAAGFEVSEKDRCLQCFDLTFDVSVQSFLLPLMHGACTYTIPHDQIK
                     YSYVYGLMEDHQLSFIVMAPSMIRYLRPYFDEIDVPSVRYCILTAEASPLDLVDEWAA
                     CIPNAEILDFYGPTEATIYCTYYRYKRNGVNKQLNGMLSIGVPMEGVTGLILDDQRNI
                     LGKGEKGELCIAGGQLTLGYWKNEEKNAEAFFSMEYEGTPTRFYKTGDLCFYDAEGDI
                     LYSGRMDFQVKIQGYRIELGEIEHHAREFMQGNNAVAIPFQNTSGNTEIALFLEGEEE
                     SPARVVQFLKTKLPAYMVPTKFIFKPLLPLNTNGKIDRKALKAFLD"
     gene            8954..9886
                     /locus_tag="PLU11_03550"
     CDS             8954..9886
                     /locus_tag="PLU11_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPN58217.1"
                     /translation="MKFGVIGNDQMLLSCLRILLDRPDAEISFVIYDTRRINPMNPIG
                     DFCEKHKLNHKGIERLNEPEMLHFIQQQQPDYILSINNFWVIRPDVLAVPARGTINFH
                     NSVPSRYHGLNVPSWVIMNGEKTHGAMWHFVEPAIDTGEVLLFREFELAKGETAASLM
                     VKCIRAGIELFPELLDQLYTGNIQRIPQQPNASYFGKKQYPANNGYIDFSANSEVIER
                     LVRGLNYLPYANHFLYAKIRHGEKELIVNEVGLETPVTGAMPGVVLAVEEDRILVGTG
                     NGVIAIEDAMDEEGNECNGEAMAGYFGICPGDKL"
     gene            9918..10157
                     /locus_tag="PLU11_03555"
     CDS             9918..10157
                     /locus_tag="PLU11_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPN58218.1"
                     /translation="MERATVIEQLTPVFRKIFNAPELEITDEMTANDVEGWDSLSHML
                     LIADVETAFSIKFKLKELNKMRNVGDMIEIILSKA"
     gene            10160..11596
                     /locus_tag="PLU11_03560"
     CDS             10160..11596
                     /locus_tag="PLU11_03560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPN58219.1"
                     /translation="MVFTSVNFILFFAAVIVLYFSAPLKWRWLVLLVASYFFYINIKP
                     VFAILLAAVTASTYVFTRLMDQTRKESRKNVYKVINIILVLLPLFFFKYFAVINNSLI
                     AGLQGWGIHWPLPEIELFLPVGISFYTFMAIGYTIDVFNEETPAEKHPGIVALFISFF
                     PLILSGPIERAGNMLPQFRNPVNPDYDKVVKGLRFMLWGFFMKLVVADRIGIYVDAVY
                     HNIPNHNGTTLLAAFFLYPFQVYADLGGYSLVAIGVAGILGIKVMDNFRRPFFAATMA
                     EFWRRWHMSLITWLTDYVYTPISFALRQYKLWGIVYSLMITFMISGIWHAASLTFVAW
                     GLLQGIYLSVEALTNKQRANMEKKYKLANRGWYIFLSCLITFILFGISQVFARSANLQ
                     DAFQVLGKIVTEPGALFIGAPSILIFSILGISMVMLKDFTDEYYPGRFLLFENRNKPV
                     RVMAYSLVIVLILLIGVFDGGEFIYFQF"
     gene            11601..12512
                     /locus_tag="PLU11_03565"
     CDS             11601..12512
                     /locus_tag="PLU11_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58220.1"
                     /translation="MLKKLFINFLLVAAVVAVLDFALGKVLRHFYFRESSGLHYRTTY
                     SMEKADADILIFGSSRANHHYVPEVFADSLGLTFYNTGRDGNGIFYQAALLKSVLKRY
                     TPKVIILDYAGDFGKGRQVYDNLSSLLPYYQSHPEIRQEVNLRSPYESVKLLSGIYPY
                     NSQLLTIAVGNLDMNKKRKSDNKGYVPLETEHPVVKEYFNSLIQQPVDSNKIESLREF
                     VRVAKASGTRFVVVYSPLYQEHKRYWEIEICETICKEAGVPFWDYSRDSFFVQQPDLY
                     YDPVHLNHKGALIFSKQIASRMRDVLH"
     gene            12578..13603
                     /locus_tag="PLU11_03570"
     CDS             12578..13603
                     /locus_tag="PLU11_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58221.1"
                     /translation="MKIKYLLKGLLKSVPGVEYVYNFHKETGGTDNASYCYTVWMRHL
                     MMAWENGYQRIPASIAELGPGDSLGIGLSALLTGAEKYYALDVIRYSNTERNLLVFEE
                     LVKLFKAKAAPSDTEFPNMRPKLPQLHFPAHILTDEKLEKLLDEKRLDRIRSSIRACG
                     QGQPLQEDSMILYEVPWTDASVIRQESVDMIISQAVLQHVNDLPGTYRSMNAWLKKDG
                     IMSHNIDLKSMGSSDHWYGHWTYSELEWKIVKGRKKYLINREPYSVHMRLADESGFKV
                     VKEIKDIQALPDRNQFSGQYRKMKEEDMTISSLFMQAVKTRLLILFNCIVFAGEHYNV
                     YSLSTIV"
     gene            13622..14047
                     /locus_tag="PLU11_03575"
     CDS             13622..14047
                     /locus_tag="PLU11_03575"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPN58222.1"
                     /translation="MRRVIIDNKTGFLIRPEKLRRKKRQRIFVVLSLVLTIVLSSLSD
                     FHLERVLGLEYSQGFDMLQHGGYYFLLTLFLLYQLPSQQGSFSFYISLFSFSVLVESI
                     QLFTPGRTFSEFDLLSNFLGITLAFVMNNFFAGAKQKNR"
     gene            14096..14260
                     /locus_tag="PLU11_03580"
     CDS             14096..14260
                     /locus_tag="PLU11_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58223.1"
                     /translation="MGILRQIAEYLYLRKKDPNRPKDKWIGYMHGINRISLLVFLLCM
                     IILAVKLLRH"
     gene            complement(14241..>15302)
                     /gene="lpxB"
                     /locus_tag="PLU11_03585"
     CDS             complement(14241..>15302)
                     /gene="lpxB"
                     /locus_tag="PLU11_03585"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPN58224.1"
                     /translation="IREIRKNDAAARIRCWGGDKMKAQGGELVKHYRELAFMGFTEVL
                     MNLRTIFRNLAICRKDIGEYQPDVLILVDYPGFNLRIASWAKKQGIRVVYYIAPQVWA
                     WKENRVKSMKECIDLMLVILPFEKDYFKNKWNWDVHYVGHPLVEETDAAATKGSGERL
                     AEKPVIALLPGSRKQEISKKLPVMLEMSRRFPEYRFVVAKAPGTDEDFYKELLLNYPG
                     VTYVQNRTYELLLQSKAALVTSGTATLETALLGIPEVVCYKGSPISYQIARRVIKVKF
                     ISLVNLIMDREIVKELIQDAFNPDNLETELKAILTNEDRIMKMKDEYARLKALLQQGG
                     DASATAAAHISRFLSAGGA"
BASE COUNT         4175 a         3178 c         3678 g         4271 t
ORIGIN      
        1 gaagtagtgc tcaaatgtta ccaggaatat ggaattggtt ccgtggaaaa atttattggc
       61 atgtttgcat atgccatata tgatcagccc aaacaggaac tttatattct ccgtgaccgg
      121 gcgggtgtca aacccttgta ttattatcat gctaataata ccctgctttt cgcttccgag
      181 ctgaaatcat ttcatgccta tcccttattt gagaaaaaga tcgagcccca ggctgtttcc
      241 ctgtttttta aatatggtta tatccgggat ccttttacca ttttccaaaa tacacacaaa
      301 gtaagaccgg ggcattacat aaaaatagat atccgtacaa agacagtaac cgatcataaa
      361 tactgggatg tggtggaaca ctacaataaa cccaagattc agatttctga agaagaagca
      421 acagcagaac tggaaaaatt attccgttcc gcttttgaat accgcatggt gagtgatgtg
      481 ccggttgggg tattcctgag cggcgggtac gacagtacgc tggttactgc aattctgcag
      541 gccaacagtg cccagaaaat taaaacgttc acgatcgggt ttcatgagaa aaaatggaat
      601 gaagctccct atgccaagga gatcgcggcc cacctgggta ccgatcacca tgaatactac
      661 tgtaccacca aagaagcaca ggatatattt ccccaactgg cagatatata tgatgaacca
      721 tttggtgaca gctcgggtat accaaccacc ctcgtgagcc gctttgcccg caaacaggtt
      781 acggtcagtc tctccgcaga tggaggggat gagatatttg caggatacaa taagtaccag
      841 caaatgcaga agctgaacaa ttacctgctg agtgcgccag gaccgctgct ggcactgggc
      901 aagggcgcgg caaatttgtc catgaaagcc atccccggtc ttcgccggtt ccataaggtg
      961 gaaagactga cggaaattct gaatacgtac cggaatgccg gactttccga tatctacagc
     1021 cggcaataca agaataatga cctgtatgac ttattgcccg gatacaatga taagcttgga
     1081 ctttttgatg agatggagat cctgaatagc ggaaatgatt ttatcaataa aagcctggcc
     1141 tatgactacc gcacctatat ggtggatgat atcctgacca aagtagaccg ggctacgatg
     1201 agtgtggcac tggaaggacg ggagccttta ctggatcacc ggattattga attcgcatcg
     1261 caattaccat ccaacctcaa gtaccataac ggtgaaaaaa aatacctcgt taaaaagatc
     1321 gtgcacaagt atattcccaa ggaagtgatg gaccgtccga aaacagggtt ttcaattcca
     1381 gtatttgact ggctgaccgg tgatctgaaa gatcatttgt acagtctgat caatgaaaag
     1441 gaatttgcca aacatggcct gattgatatt aaaaaagcca tgagcgtacg gaaggagttt
     1501 gaagatggac ggaaaggaag agatgcgcag atctggctcc tgctgatgtt ccagatgtgg
     1561 tggaaccgct ggatgtaaca tgcaatacca agctgatgaa taaactatac taacctggcc
     1621 ggaatgaaga ttctaattgt aaattatcgg ttttttgtat ccggggggcc agaacgctac
     1681 ctgttcaata tcatggcgct tcttaaaaag aagggccaca ccgttatacc ctttagtgtc
     1741 agcaaccagc ataatcaacc ttccgattat agggattatt ttatgagccc ggtgggtgaa
     1801 gggaaagaag tatacttcaa cgaatacagc aaacgtaatc tgaaaacact ctggaagacc
     1861 ttttcccgta tgttttattc cgttgaagcc aggcggcaac tcagccggct cataaaggat
     1921 acacagcctg acctggttta cgtgcttcat taccagaata aaatgtcagc cagtgtgttt
     1981 gatgccgcgg cccggcttaa ggtaccggtg gttcaccggg tttccgattt cagccagatt
     2041 tgtgcaaacg gattgtttta tcgtcctcag caaaaagatg tgtgtgaacg atgtctgcat
     2101 ggcacaagac tgaatgccgt gaagcataaa tgtgtgcatg attcttattt cttgtctgcc
     2161 gttaaggccg cttcactgaa attgcaacaa ttcctgcacc tggataaaaa gattgccgct
     2221 tatgtggttc cttccggatt tacgatcagc aaactggctg tatttggttt gccccccgcg
     2281 aaactccggc atatccctac cttcttcaac ttcagtatca tgaaagagaa cctggcgatc
     2341 agctatggtt catatgcttt atttgttggc cgggtggaag aggagaaagg gttgtataca
     2401 atggtgaatg cgtttatcga taccccttat tcactgaaga taatcggggg atcatcttcc
     2461 ggatttgatg ctaaactaaa atcatacctg gctggtaaaa atcaccatat tgaattgctg
     2521 ggccaactgg ctttcagtga gatccagcaa tacctggcgg cctgcgcctt taccgttatg
     2581 cccaccgaag tgtatgacaa ttttcccaac acggtgctcg aaagttttgc tttccgcaaa
     2641 gcagtggtgg caacgaatgt gggcagtctg cgggagattg taaatgacca ggagaacgga
     2701 ttgttattta cactgaaaga tgcggtatcg ctgagaaact gtgtgcaatc gctttttgag
     2761 gattcggata aatgcaggcg gttaggggaa caggcgttcc gcaaactgaa tgatgaatac
     2821 tcggaggagc aacattatac taaactgatg cattgttttg aaacagtggt ggatgaggtc
     2881 cggcaaaaac cggtactggt acaggcagca aaaacattcg taaactgaac agggtgagaa
     2941 taattatact tgcggatttt aaggtaaagg ccggctcaga cttctggatc agggaggaac
     3001 tggaaaaact cggctatacc acggagctcc tgggtattca gaattacaat ccgaaggatg
     3061 ataccacccg gttcggtaaa attaaaatct ggtataaata ttttaaactg gctggtaaag
     3121 gcctgtcagt ctcaggtaag gaagatgtgc tgatcagtga caatttcgta attggtgcca
     3181 ttaccgcttt tttcggccgg ctcagtggcc ggaaaagaaa agtaatcgcc ctgaatatga
     3241 ttgcccatga aaaggggctt tttaaccgta ttttccggaa aatcgtttac aatacggctt
     3301 ttcgtaaccg gggtttctgg ttttcggtga atgacagtca attaatagac ctgtatgcac
     3361 agcaattccg ttttccggaa gaccgggtgt ttgttttgca ggatccgttt tttaaaagtg
     3421 atgagcaggc agactttacc ggtgaagggg attttgtttt tacaggcggg gatgcctacc
     3481 gggattggga tgcagtaatc cgttgtgcaa aggaactccc gcagatccgg tttaccgggg
     3541 tggcgaggaa gaagtatttt ccaggacaca cggagttgcc cattaacctg aaaatgtatt
     3601 ttgataccac gcaggacgaa ttttacgggt tgatgaaaaa cagccgcctt gtgtttttgc
     3661 cattgaacag taaagcacct tgcggactga ttgttatgat gagggcggca ctgttgtcta
     3721 agccggtggt gatttcagaa acgccatcca cgcagaatta tatccggaat aagatttccg
     3781 ggatattgct gcccttgcag gatcagttgg gaatgaaaaa agagatatcg gaattattct
     3841 ggagccagga gttgcagcaa aagtatgcgg ccgaactgaa gcattttatc cttgaaaatt
     3901 tttcaaccgg ctccaccatg aaaaaaattg aagaaataat aaaaaaataa gaattataaa
     3961 ccgaaaccta aaatgaataa catgaaaaag agaatttaca tggccggatg tgggggaatg
     4021 ctgggagagg cattttatac ccagttcaag gatgattatg agattaagtg caccgacaag
     4081 gatgtgaacg tatcctggct gagtttcctt gatttccggg attttgatgc ctataagaaa
     4141 gatgtgctgg atttcaagcc ggattatctt tttcacctgg gagcttatac cgatctggag
     4201 ttctgcgagc aaaacgcgga ggatacttat aaaaccaata ccctggccgt agagaatgca
     4261 gtttacctgg cgaatcaact tgatattccc ctgttataca tcagtacagc tggaattttc
     4321 gatgggaaaa aggagttgta tgatgattgg gaccagccta atcccctggg tgtttatgcc
     4381 cgttctaaat atatgggaga acgctatgta gtggagaacg cccggcggtt cctggtatgt
     4441 cgtgccgggt ggatgatggg agccggacct tcaaaggata agaaatttgt tcaaaagctg
     4501 atgaaacagc tgaaagaggg gaaaaaggaa ttgtttattg tggatgacaa ggatggtacc
     4561 cccacgttta cccatgattt tgccaggaat gtaaaagcct tgatccagaa agaatactgg
     4621 ggattatata acatggtgtg tggcgggcaa accagccggc tggaagtagc aggagaactg
     4681 ctgaaattaa tggggttgga aaaagaagta aaaatcacca gtgtgaattc tgattatttc
     4741 aagcagactt attttgccga acgtccgcct tgcgaacgcc tggtaaacag gaaactggag
     4801 ctgagagaac tcaacctgat gcaggactgg aaatcagcct tacatcagta cgtgaatgcc
     4861 tactacaagg attatcttaa ataagcatga agaagctcag aattgccgct tttggtttcc
     4921 gcacccttcc tcccgccaag ggagcggcag gggcagataa atttgcgatg gaattatttc
     4981 cccgcctggt acaacgtggc catacggtaa cggcgtataa ccgccgttac ccggatgtgc
     5041 accaggacat aaccgagtat aaaggggttc ggattaaaac ctttaaaaca gttaaccgga
     5101 aaggatttga tacgctttta cactcattga agtgtacctg tcatattatt ttcaggaaca
     5161 cagccgatgt ggtacatatt cagaatggag gtaacagtat atgggcactg ccactccgac
     5221 ttttcgggaa gaaagtgttt atatcccagg acggagttga ctggaaaagg gataaatggc
     5281 cctggtatgg taagttgtat ctccggattt ccacttatat cagtgcgtat ttcccgagcg
     5341 atatcatttt tgacaatgtg attgcccgcc ggtattttga agagcgcttc aaaaaaaaat
     5401 ttgcctttat tccttttgga agtgaagtgg caccggtagt tcctggagag gaagtgttta
     5461 cacgttttgg actggagaaa gacagctatt acctgtttgt gggtcgcttt atcccggata
     5521 aaggactcca ttacctgata cctgcatttg agaaatcagg atccaggcga aaactggtaa
     5581 tgattggagg ttcccctaat ccttcttcct atgagaatga actctatgct atggcctcgg
     5641 ataaagtggt attcaccggc ttcctgtatg gagatgatgt aacccggctc atgaagaact
     5701 gttattgtta tatacagcct tcagatgtgg aagggctttc ccctgtcatt ctttccgtga
     5761 tggggctggg cgttccactt attgtcagtg acattgaaga gaatatctat gctgtacagg
     5821 atacggccat aactttcagc aaggccgata ccaattcatt gacggagaag atcaggtatg
     5881 cagaacagca ccacccccat atgaaggaac tggcggtgaa agcacaaaac agggcattga
     5941 ctgaatttaa ctgggataat gtggctgagc aacatgaaca gatattttca ggtataaagc
     6001 aggtacgtta aagaaccagg aaaaaagtga ttgcatgaca aacaaaaata aaggcatatt
     6061 ccagctgttt tttgcaggga tggacttgtt cgccctgaac gcggtgcacc tggcactgat
     6121 catggtgatg aaccggattg atcgtagcgg ccagcaatac aatgcttttt ttattgtaag
     6181 caatatggcg tggctggcct cggcctatat taatgcggta tatgcccgga ccaatcgttt
     6241 caactttgat tattttggaa agcagtcgtt gaaagcctac ctgttgtttg tcattttcat
     6301 cctgtttttc atctttattt ataattacag ttattcaagg ctttttatca tatccaattt
     6361 ccttggattt ggcctgatca ttcttttcac ccgtctgctc tttcttttac tgagtgtata
     6421 catgcggaaa ggcaaccggt ttaagaaaaa agtggtggtg cttggataca atgatctttc
     6481 caaaagactg gtcgggcatt ttatggtcag tgaccagttg tctatggaag gatattttga
     6541 agacccacag gaagtacacg aactttccac tttcccgatc ctgggcaacc gaaaggaatg
     6601 tgtgtcctat gccatcgaga atgaagtgga tgaaatctac tcaacggttt cccctgaatc
     6661 taataagtat gtatatgata tggcccaggt ggcggaatcc aatatgatcc ggtttaagtt
     6721 tgttcctgat ttccagctat ttgtcaaccg gaatacacat attgatttca tcaatgatat
     6781 acctatttta tcccttcgtt ctgagccatt ggatgatatc actgcaagga ttaagaaaag
     6841 ggtcttcgat attgtattca gtacaatggt ggccgtattg cttttgtcct ggctggtgcc
     6901 gctgatggct atcctgatta aactcagttc caggggacct gttttctttg tgcagttacg
     6961 atctggacgg gataataaat cttttaagtg ctttaagttc aggacccttc gggtcaacag
     7021 ggaagcggac accaagcagg taacacagaa tgataaccgg ataacccggt taggcgcctt
     7081 cctgcgaaaa tcaaatattg atgaattgcc acagttcttt aatgtgatca tcggtaatat
     7141 gtccgtggtt ggcccccggc cacatatgct ccggcatact gaggattatt ccagggtgct
     7201 gaatgaatac atgatccggc attttgttaa acccggcgtt accggctggg cgcaggtgaa
     7261 tggtttccgt ggggagattc gtgaaaaaga acagttgcgg aagcggattg agtatgatat
     7321 ctggtacctg gaaaactgga acctctggtt tgacctgcgg gtgattttcc tgactgtttg
     7381 ggtgaccatt aaaggagata aaaacgccta ttgaccggtt catatgaatt ataaatatgt
     7441 ataactatgt ttatcgaaaa aataataaat gcccttaccc gtcagatcag ccaccggcct
     7501 gatcatatgg ccttttgtat agccggggaa catattacct atgccggatt tggcaatcag
     7561 atcgccacga tccgccaggc actccgtacg attgacaata aaaaccataa tgtcggactg
     7621 gttaccaatg atgatataca aacctatgct tccatttttg ccttatggat ggaagggcat
     7681 gcctatgtgc cccttcatca cacccaaccg gcagagcgaa gccatgaaat tattggtcag
     7741 gctgaaatca gcctcgtatt aaattcgctt ccgggtggtt cattccaggg tgcccggtta
     7801 ttaattacaa aagatttgcc tgctgcttcc gtattacatg aacccgtcgt tgttccggat
     7861 tcagcactgg cttatatatt atttacttcc ggcagtaccg gcatacccaa aggggtgccg
     7921 attaccagga gtaacctggt cgtattcatg gatgcattta tggcagccgg atttgaagta
     7981 tccgaaaagg accgctgcct gcagtgcttt gatctcacat ttgatgtatc tgtacagtca
     8041 ttcctgttgc cgctcatgca tggggcctgc acctatacca tacctcatga tcagatcaag
     8101 tatagctatg tttatggatt gatggaagat catcagctca gctttatcgt aatggcgccc
     8161 tccatgatca ggtatctccg gccgtacttt gatgaaattg acgtgccaag tgtccggtat
     8221 tgtatcctta cggcggaagc ctctccgctg gacctggtgg atgaatgggc cgcttgtatt
     8281 cccaatgcgg aaatacttga tttttacggc ccgacagaag ccaccatcta ctgtacgtat
     8341 taccggtata aacgtaacgg ggtcaataag caattgaatg gcatgctctc tatcggtgtg
     8401 ccaatggaag gggtaaccgg actgatcctg gatgatcagc ggaatatcct cggaaaggga
     8461 gaaaagggag aactctgtat tgccggggga cagctgacac tcggttactg gaaaaatgaa
     8521 gaaaagaatg ccgaggcctt ctttagtatg gagtatgaag gaacacccac ccgtttttac
     8581 aaaaccgggg atctttgttt ttatgatgct gaaggtgata tcctttactc ggggcggatg
     8641 gatttccagg taaaaataca aggataccgg atcgaacttg gggaaataga acaccatgcc
     8701 agggaattta tgcagggaaa taatgctgtg gcaattccat ttcagaatac tagcggaaat
     8761 acggaaattg cactcttcct ggaaggggag gaggaaagtc cggcccgggt agtgcagttc
     8821 cttaaaacaa aactgcccgc ttatatggtg ccgactaagt ttatttttaa accccttctt
     8881 cccctgaata ccaatgggaa gatcgaccgg aaagctctca aagcattcct ggattaataa
     8941 agaagagccg gatatgaaat ttggagtaat tggaaatgac cagatgttgc tgagctgttt
     9001 acgtatcctg ctggaccggc cggatgcgga gatcagtttt gtgatctatg atacccgacg
     9061 gatcaatccc atgaatccga tcggggattt ctgtgaaaag cataagctga atcacaaagg
     9121 tattgaacgg ctaaatgagc cggaaatgct ccattttata caacagcaac aaccggatta
     9181 tatcttaagc attaataatt tttgggtgat ccggcccgat gtactggccg taccagccag
     9241 gggaaccatc aattttcata attcagtccc gtcccgctat catggattga atgtgccctc
     9301 ctgggtgatt atgaacggag agaaaacgca tggagcgatg tggcattttg tggaaccggc
     9361 tatagatacc ggggaagtgc tcctgtttcg tgaatttgaa ttggcaaaag gggagacagc
     9421 cgcgtcgttg atggtaaaat gcatccgggc gggaattgag ctgtttcctg aattgctgga
     9481 tcaattgtat accggaaata tccagcggat tccacaacag cccaatgctt cctattttgg
     9541 aaaaaagcaa tacccggcaa acaacgggta tattgatttt tcggccaatt cagaagtaat
     9601 agagcgtttg gtgcgtggac tgaactatct gccttatgcc aatcattttc tctatgcgaa
     9661 gatcaggcac ggggagaagg aattgatcgt gaatgaggtg gggctggaaa ccccggttac
     9721 cggagctatg ccgggtgtgg tgctggcggt ggaggaagac cggatattgg tgggaaccgg
     9781 taatggcgtg attgccattg aggatgccat ggatgaagag gggaatgagt gtaacgggga
     9841 agcgatggcc ggttatttcg gtatatgccc gggggataaa ttataatgtg aaacggagaa
     9901 cttattaaat atacactatg gaaagagcaa cagttattga gcaactgaca ccggttttca
     9961 gaaagatttt taatgcaccg gaacttgaga tcacagatga aatgaccgcc aatgatgtgg
    10021 aaggatggga ctctctttct cacatgttac tgattgcaga tgtggaaact gctttttcaa
    10081 tcaaattcaa actgaaagag ttgaataaaa tgcggaatgt gggtgatatg attgaaatta
    10141 ttttgtcaaa agcataggaa tggtttttac atcggtaaac tttatcctgt tttttgctgc
    10201 ggtgattgta ctgtatttca gtgccccgtt gaaatggcgc tggctggtct tgctggttgc
    10261 cagttatttt ttttatatca acatcaaacc ggtttttgcc atactattgg ctgcggttac
    10321 cgcctctacg tatgttttta cccggttaat ggatcagacc cgcaaagagt cacgaaaaaa
    10381 tgtttacaag gtcatcaata tcatactggt actgttgccc ttgtttttct ttaagtattt
    10441 tgccgttatt aataacagcc tgatagcagg tcttcagggt tggggtattc actggccgct
    10501 tcccgaaatt gaattattcc tgccggtggg aatttccttt tataccttca tggccatcgg
    10561 ttataccatt gatgtcttca atgaagaaac acccgcagaa aagcacccgg gtattgtggc
    10621 cctgtttatt tcttttttcc cattgatttt atcaggtccc attgaaaggg ctggcaatat
    10681 gctgccccag ttccggaatc ctgtcaaccc ggactatgac aaagtggtga aaggactccg
    10741 atttatgctt tggggctttt ttatgaaatt ggtggtggcc gaccggatcg gcatttatgt
    10801 agatgcggta taccataata ttccaaatca taatggcaca accctgctgg cagcattttt
    10861 tctttatcca ttccaggttt atgccgatct gggtggatac tcactggttg ccatcggtgt
    10921 ggcaggtata ttagggatta aggtcatgga taatttcaga cggcccttct ttgcagctac
    10981 catggctgaa ttctggagaa gatggcatat gtcactgatt acgtggctta cagattatgt
    11041 gtacacccca atttcattcg cgctccgcca atataaatta tggggtattg tttattcgct
    11101 gatgatcact tttatgatca gcgggatctg gcatgctgcc tcgctcacct ttgtcgcctg
    11161 ggggctctta cagggaatct atctcagcgt agaagcgctg accaataaac agcgggcaaa
    11221 tatggagaag aaatataagc tggcaaacag gggctggtat atcttcctgt cctgcctgat
    11281 tacttttatt ctctttggta tctcccaggt ttttgcacgt tctgccaacc tccaggatgc
    11341 tttccaggtg ctgggaaaaa tagttactga accgggagcg ctttttatcg gggcgccttc
    11401 tattttgatt ttcagtatac tgggtatcag tatggtcatg ttgaaagatt ttacagatga
    11461 atactatccg ggccgctttc ttttatttga gaaccggaac aaaccggtcc gtgttatggc
    11521 ttatagttta gtgattgtcc tgattctcct gatcggtgta tttgatggcg gggagtttat
    11581 ttattttcaa ttctgacggg atgttaaaaa aactttttat aaacttctta ttggtggctg
    11641 ccgtggtggc ggtgctggac tttgctttag gtaaagtact ccggcatttt tatttccggg
    11701 aatcttccgg tctgcattac cgcaccacat attcgatgga aaaggccgat gcggatatac
    11761 tgatctttgg ttcttccagg gccaaccatc actatgtacc cgaagtattt gcggactcac
    11821 tgggacttac cttttataat accgggcgtg atggtaacgg aattttttac caggccgcac
    11881 tgctcaaatc cgttttaaag agatacactc ctaaagtcat catattggat tatgccggtg
    11941 attttggcaa aggacgacag gtgtatgata acctgtcttc tttattgcct tactatcaat
    12001 cacacccgga gatccgccag gaagtaaacc tgcgcagccc gtatgaatca gtgaaattgc
    12061 tttcaggtat ttatccctat aattctcaat tattgacgat tgcggttgga aacctggaca
    12121 tgaataaaaa gcgaaagtct gataacaaag gatatgtacc gctggagaca gaacatccgg
    12181 ttgtaaaaga gtattttaat tcactgatac aacaaccggt ggattccaat aagatcgaat
    12241 cgttgcgtga atttgttcgt gtggcaaaag catcgggtac ccgctttgtg gtggtctatt
    12301 cgccacttta tcaggaacat aagcgctatt gggaaattga aatatgtgaa accatctgta
    12361 aagaggcagg tgttcctttc tgggactatt cccgggattc tttttttgta caacagcctg
    12421 atttgtatta cgatccggta cacctgaatc ataaaggtgc cctgattttt tcaaaacaaa
    12481 tcgcatccag aatgcgggac gttttgcatt gaatcagaat ccttaaattg tattataagt
    12541 agtattttac ctgttcatta agcctaacac aaaaaccatg aaaattaaat acctgctgaa
    12601 aggtcttctg aagagtgtac ccggggtgga atatgtttat aatttccaca aagaaaccgg
    12661 cggtacagat aatgccagct actgttatac ggtctggatg cgacacctga tgatggcctg
    12721 ggaaaacggg tatcagcgta taccggcatc catagctgaa ctggggcccg gcgattcact
    12781 ggggatcggg ctttctgcct tgctcaccgg ggcagaaaag tattatgcac tggatgtgat
    12841 ccggtattca aacaccgaac gtaacctgtt agtatttgaa gaacttgtaa aacttttcaa
    12901 ggcaaaagcg gcgccatctg atactgaatt cccgaatatg cgtccgaaac tgccgcaatt
    12961 acatttcccg gcacatatcc tgacggatga aaaactggaa aaactgctgg atgaaaaacg
    13021 gttggatcgg atacggtctt cgatcagggc ttgtggtcag ggtcagcctt tacaggaaga
    13081 tagcatgatc ctttacgagg ttccctggac agacgcctcc gttatacgcc aggaatcagt
    13141 ggatatgatt atttcgcagg cagtactgca acatgtgaat gatcttccgg ggacgtatcg
    13201 cagcatgaac gcctggctta agaaagatgg tattatgtcc cataatatcg acttgaaatc
    13261 aatggggtcg tccgatcatt ggtatggaca ttggacctat tctgaactgg aatggaaaat
    13321 agtcaaagga cggaaaaaat acctgattaa ccgggaaccc tattcggtgc atatgcggct
    13381 ggcggatgaa agcggcttta aagtggttaa agagattaag gatattcagg cattacccga
    13441 caggaatcag ttttccggtc agtatcgcaa aatgaaagag gaggatatga ccatttccag
    13501 tttgtttatg caggcggtta aaacgaggtt gctgatattg tttaattgta tcgtatttgc
    13561 cggagaacat tacaatgtgt acagtctcag taccattgtt taaattataa aataagaaga
    13621 attgcgccgg gtaatcattg ataataaaac agggttcctt atcaggccgg agaaactgcg
    13681 tcggaaaaaa agacagcgca tttttgttgt tctttcgctg gtgctgacta ttgtactcag
    13741 ttcattgtcc gattttcacc tggaacgtgt tcttggtctt gaatacagcc agggatttga
    13801 tatgcttcag catggggggt attattttct gctgacccta ttcctgcttt accagctgcc
    13861 ctcccagcaa gggtcctttt ccttttatat ctcactgttt tccttttctg tactggtgga
    13921 aagcatacag ttattcacgc ctggccggac cttctcagaa tttgatctgt tgagcaattt
    13981 tttgggaatt actcttgcat ttgtaatgaa taattttttt gcaggagcta aacaaaaaaa
    14041 ccgttaggcg gttatttgtt ataaagcctg tcagacattt tgttaatttt gcaacatggg
    14101 tatcctcaga caaatagccg aatacctcta cctccgtaaa aaagatccca accggccaaa
    14161 agataagtgg atcgggtata tgcatggcat caaccggatc agccttttgg tattcctgct
    14221 ttgtatgatc attctggcgg ttaagctcct ccggcactga gaaaccggct gatatgcgcc
    14281 gccgctgttg ctgaagcatc acctccttgc tgcagcaggg cttttaacct tgcatattca
    14341 tccttcattt tcataatccg atcctcatta gtgaggatgg ccttcaattc agtttcgaga
    14401 ttgtccggat tgaaagcgtc ctgaatcaac tcctttacaa tttcccggtc cataatgagg
    14461 ttgaccaggg agataaattt cactttaatc acacgcctgg cgatctggta cgaaatgggg
    14521 gagcctttat aacagactac ttccgggata cccagcaagg cggtttccag ggtagccgtg
    14581 ccggaagtga ccagcgctgc ttttgattgc agcagcaatt cataggtccg gttctgcaca
    14641 taggttacac ccgggtaatt cagcagcagt tccttataaa aatcttcatc agtcccgggc
    14701 gctttggcta ctacaaaccg gtattccgga aaacggcggc tcatctccag catgaccggc
    14761 agttttttac tgatctcctg tttacggctg ccaggtaata aggctatcac cggtttttcc
    14821 gccaaccgtt ctcccgatcc ttttgtggcg gctgcatcgg tttcttccac cagcggatgc
    14881 cccacatagt gaacatccca gttccattta ttcttaaagt aatctttttc gaaggggaga
    14941 atcaccagca tcaggtcaat acattccttc atcgatttta cccggttctc tttccaggcc
    15001 catacctgtg gtgcgatata gtaaacgacc cggatgcctt gtttttttgc ccagctggcg
    15061 atccgtaaat taaaacccgg atagtccacc aggatcagta catccggttg atattcccct
    15121 atatccttac ggcagatagc aaggttgcgg aaaatagtac gcagattcat caacacttct
    15181 gtaaatccca taaaagccag ttcccggtag tgcttcacga gttcgccacc ctgggctttc
    15241 attttatctc caccccagca acgtatccgg gctgcagcat cgtttttacg gatttcccgg
    15301 at
//
LOCUS       DAOPMW010000039        15035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_3970_length_15035_cov_8.711282, whole genome shotgun sequence.
ACCESSION   DAOPMW010000039 DAOPMW010000000
VERSION     DAOPMW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_15035_cov_8.711282"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="PLU11_03590"
     CDS             complement(<1..1717)
                     /locus_tag="PLU11_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPN58225.1"
                     /translation="MSEALHVHSGAAHAVHHDDHGHGHHEQSFISKYIFSMDHKTIGK
                     QFLITGIVWAIIGGLFSVLFRLQLGYPDQSFPILETFFGHWAKGGQIQPEFYYGLVTI
                     HGTVLVFFVLTAGLSGTFANLLIPLQIGARDMASPFMNMLSYWFFFLASVVMLSSIFV
                     HTGPASGGWTMYPPLSALRQAGDGQKIGTDLWLIAMALFIISSLLGGLNYISTILNMR
                     TKGMSMTRMPLPIWAMFFTAVLGVLSFPVLLSGAILLLFDRNLGTSFYLSDIVINGEI
                     LPNEGGSAILYQHLFWFLGHPEVYIILLPAMGMVSEILSVSARKPIFGYMAMLGSMFA
                     IAILAFLVWAHHMFVTGLNPFLGSVFVLLTLLIAVPSAIKVFNWLTTLWRGNIRFTPA
                     ALFAIGFVSLFISGGLTGIFLGNSTIDIHLHDTMFVVAHFHIVMGVASFFGMFAGVYH
                     WFPKMFGRYLNNTLGYLHFWVTMIGAYLIFWPMHYQGLAGVPRRYLDKKGWVSFNQFG
                     DLDKMITVVSIIVFAVQLMFVFNFFYSIFKGRKVTTTNPWGANTLEWTTPINPGHGNW
                     EGEIPE"
     gene            complement(1759..2805)
                     /locus_tag="PLU11_03595"
     CDS             complement(1759..2805)
                     /locus_tag="PLU11_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPN58226.1"
                     /translation="MQTFFIVAILALGFVITFQIAKASEYVAVLRGYERSRKQSNKIN
                     GFLLLAFLILGLIGVYYCNEQLKGKILGEPASDHGVLVDRMLYVTIAITFIVFVITQV
                     ALFWFSYKYQEKENQKAFYFPHNNKLELIWTVIPAIVLTILVGVGIFYWFKITGAAPK
                     NAMQVEIVGKQFGWEMRYPGKDGVLGKKYFKNVNPAANNPAGQIWDDPANYDDVFVEQ
                     EMHLVVGKPVQLVIGAKDVIHDVGLAHFRLKMDAVPGTPTTMWFTPTITTADMRKKTG
                     NPDFVYEISCDQMCGKGHWSMRGTIVVETQEEFDAWMAGKKPQYLVANPDKDPSKKAD
                     TLSAAAPVTGAIIR"
     gene            complement(2883..>3176)
                     /locus_tag="PLU11_03600"
     CDS             complement(2883..>3176)
                     /locus_tag="PLU11_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HPN58227.1"
                     /translation="APLLILMTRDAKRNYGTITFMAIMIIFGHWLDFYQMVFPSVMKD
                     STGATHVPNILYDMGVALGFVGLIMFVTGRALSKAPLLAKNHPFVKESLIHHT"
     assembly_gap    3177..3276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3277..3995)
                     /locus_tag="PLU11_03605"
     CDS             complement(<3277..3995)
                     /locus_tag="PLU11_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HPN58228.1"
                     /translation="MSIRENFEVPKRYNTIAIALMAVGILAIAGLWVTSGSSSDPHKS
                     ARFWASLLQNSVFFLLVVNASMFFICATTLAWGGWQMAFRRVPEAISAVVPVIGVIAG
                     AILLAIAFGGNHTIYHWTDTHHVAEDPILSHKKGFLNTTFFAVATILTIVLWSVLGWK
                     MRQLSRSLDNNPITTVEGRKKYVWNNTVWAALFIVVFSLTVMSSIPWLWLMSIDAHWY
                     STMYSWYTFASTFVSGMALIAL"
     gene            complement(4030..4677)
                     /locus_tag="PLU11_03610"
     CDS             complement(4030..4677)
                     /locus_tag="PLU11_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPN58229.1"
                     /translation="MKKILFVTGSLALMVAMISCGGPRTDEGRAYMPDMSYSRAYETY
                     ASTEALDKAGVHYTAKPVDGTIARGEMAAYPYQNDSIGYAKSAEVKNPLDTASRDMKE
                     AERLYLINCGICHGTKLDGNGPLYTSGVFPAAPRNLVGDPLMVAMTEGTMFHSLTYGK
                     NTMGSYASQLSTKQRWMITAYVKAEQAKKTGTAAPAATAAPAPAAKDSAAVAPMK"
     gene            complement(4705..>5284)
                     /locus_tag="PLU11_03615"
     CDS             complement(4705..>5284)
                     /locus_tag="PLU11_03615"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HPN58230.1"
                     /translation="MAVKKFVVGNFDDEAVLFPAVKKVRRAGYKIHDVFTPFPIHGLD
                     HAMGLRDTSLHTAGFIYGITGTSTAVGFITWALTVDWQINFGGKPFFSLPAWIPVTFE
                     LTVLFAAVGMVLTFCYLCQLAPFVKKDHFNPRSTDDTFVMALECTDKTNEAEVLSFLE
                     TVGAKEVKIEYKETEWWIGRYDKDKKLFHKEN"
     assembly_gap    5285..5384
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5385..6787)
                     /gene="nrfD"
                     /locus_tag="PLU11_03620"
     CDS             complement(<5385..6787)
                     /gene="nrfD"
                     /locus_tag="PLU11_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPN58231.1"
                     /translation="MALLRYESQVRAPLVDGTKDYHQVTEDICRPVEASPSRLWWVGF
                     LISVALLIFGIVSVTMEVMYGTGQWNLNKTIGWGWDITNFVWWVGIGHAGTLISAILL
                     LFRQGWRTGVNRAAEAMTIFAVMCAGQFPIWHMGRVWNAFFVLPYPNTRGPLWVNFNS
                     PLLWDVFAISTYFTVSLLFWYSGLLPDLATLRDRAKAKWRKHFYGVAAFGWTGSTKHW
                     QRHEALSLVLAGLSTPLVLSVHTIVSFDFATSIVPGWHTTIFPPYFVAGAIFSGFAMV
                     QTLLVVTRKVLALEDYITMEHIEVMNKVIVLTGSIVGIAYLTELFMAWYSAVSYEEFA
                     FFENRLNLNSPYGWSYWLMMGCNVLSPQIFWFRKMRRNLFVTFFMSILVNIGMWFERF
                     VIIVTSVYRDYLPSSWSTYYTPSIWEIGFYMGTFGLFFTCYFLFSKFFPVIALAEIKH
                     ILKKNGKSFKDHMEEIEG"
     gene            complement(6850..10056)
                     /locus_tag="PLU11_03625"
     CDS             complement(6850..10056)
                     /locus_tag="PLU11_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HPN58232.1"
                     /translation="MSQKKYWQSFGEVNDSENFQKKQQDEFREELPFEGFDDKGLIDA
                     KAPRRDFLKYLGFSTAAASLAASCKIPVKKAIPYANKPENLIPGVAQYYATTYVQDGD
                     VVPVLAKVRDGRPIKIEGNTDCGYTGGGTTARVQASVLDLYDTSRLRFPIQKTGTGEA
                     AKYNEVPTFEQFDKLVAEAMKAAGGTTVLLTSTIVSPSTKEIIAAFPGLKHVQVDPVS
                     YSGMLLANEASGFGRKIPTYDFGAAKVIVSLGADFLGTWLNAAENAKGYAAGRKIDEQ
                     HFAMSKHYQFESFLSLTGASADERFTHRPSEEGAVALELLNALTGEASTRPGIKKVAE
                     DLKANMGTALVVSGSNDKNVQVIVNAINNAIGAFGKTIDWSKPVNYRQGIDSDFAQLL
                     ADMEAGSVGTLMIYGANPAYTSTDAARFTAALAKVKVKVSFNERMDETTELCDFILPT
                     HHYLESWGDAEAKTGAVSFQQPTIYPLFKTRPFQTSLLKWSGNNTEYETYFKNYWATK
                     LGAEDVFNSILQIGVKEEPLTAAGAGAYNGVAVIEASAAIAAAKKGTGTEVVLYQKTS
                     IGTGTGATNPWLQELPDGITKATWDNYALISLTMAKSLLGLNLIDGSEKESNNYEYFP
                     QKPVIKLTVVGVNTNNKPVDIELPVLVIPGMDANTIAVAVGYGRSENLGKTAAGVGKN
                     VYPFSSYNGSTVVYHNAVVAADAGRKEKIAQTQIHNSYEDRIEVVRETTLATLKKYPT
                     VIPDYRNHLEEDFGTSKDGKTKDYRKEATLYPMHDQPGIKWGMNIDMNACYGCGACVV
                     ACHAENNVPVVGKSEVLRYHDMHWLRIDRYFVSDEKNPDQLKGVVFQPMMCQHCDNAP
                     CENVCPVAATNHSAEGVNQMTYNRCIGTRYCANNCPFKVRRFNWADYTGADSFDDNQD
                     QQLVGKLDPAVFQMNDDLTRMVLNPDVTVRSRGVIEKCSFCFQRLQAAKLEAKKQDRP
                     LKDGDAKTACQTACAANAIVFGNVHDTSSEITKVRKENPSRSFYVLEQLHVLPNVSYL
                     AKVRNTDELIEPAHHAAPAAEKTEGHAPAETKPAEAHH"
     gene            complement(10117..11394)
                     /locus_tag="PLU11_03630"
     CDS             complement(10117..11394)
                     /locus_tag="PLU11_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPN58233.1"
                     /translation="MILCKPIGQRLTVLFLSLFVISIVNISAQDAAAGKAIFTGKCAA
                     CHNVFKKMTGPALMGLEERGKWADHKELLAWINNPAAYMAKDPYTQGLKAEYGSMMTG
                     FADVTQKDVDDMVAYINAEVANKNAAPKPGAVTETADTGNQNAIIFGVISLVLGIIAL
                     ILMQVNSNLKKMSDDSEGIVRPEPVAFYRNKTYIAMASIVFFVFGGYMVAKGAIGLGR
                     QKDYQPEQPIYYSHKVHAGINQISCLYCHGNAWESKHAAIPSVNVCMNCHKAINTYEK
                     GPKLYKENGDEVNGTAEIAKLYQFAGFDPNNPSKWDPAKAKPIEWTKIHNLPDHVYFN
                     HSQHIRAGNVQCQSCHGEITAMDEVKQVADLSMGWCINCHRQTNVNFNYDSTKGNKFY
                     SIYEKFHNDIKSGKMDSVKVKDIGGLECQKCHY"
     gene            11665..11970
                     /locus_tag="PLU11_03635"
     CDS             11665..11970
                     /locus_tag="PLU11_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58234.1"
                     /translation="MFEGLQQKWKVKGGQLALILCTFAIGGSATGWVAKKIMNVLAIE
                     QDWLWAVIYIILVTICWPLMVILISIPFGQFPFFSRYIKKLGSRIGLVRSKTPDINE"
     gene            11963..12535
                     /locus_tag="PLU11_03640"
     CDS             11963..12535
                     /locus_tag="PLU11_03640"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPN58235.1"
                     /translation="MSEKLTHIAIFASGAGSNAHQIIRHFTGSAKAKISLIVCNKPGA
                     GILQIAEKEHIPTLLIEKELFFQGDAYVPELQKKGIDLIILAGFLWKIPPALIRAYPD
                     RIVNIHPALLPKYGGKGMYGRHVHQAVIENKEAVSGISIHFVDELYDNGAVIFQASCP
                     VLEGDTPESLAKRVQELEHAHFPKVIEGLL"
     gene            12604..13248
                     /locus_tag="PLU11_03645"
     CDS             12604..13248
                     /locus_tag="PLU11_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58236.1"
                     /translation="MKKLTLFFLLFASTLTSTAQNNPILITPGVGVNNIKLGMSEKQV
                     TDLLKGEPVGYTYADQLDAFKGYDTRIDSVMQFVLGFDSCIRYDNELPVSMPVFGLYF
                     KNHKLNFITVTSYSATEDHLKQVRLNNGLKFHDSMEDCGKKLAKSPFVALGYGDYSGD
                     HYYYTLGLEMVYDEDKLTAIGIYPITRDFPAKIAENSERLLKEASAEGEEEGNN"
     gene            13306..14073
                     /locus_tag="PLU11_03650"
     CDS             13306..14073
                     /locus_tag="PLU11_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN58237.1"
                     /translation="MKKLYPYIIISLLFLGAAYNSQAQTYRIKGYVLDSSRNYPMELV
                     SVISSSGKGTVTNSIGYYELEVSEQDTIWFSYLNKSTMKFPVAKISNPMQFDISIQVN
                     VPTLKEVKILPRNYRQDSIQNRLDYAKVFGFQKPKLKTVTPTYGAGVGFDLDEIINMF
                     RFRRNRSMLSFQQRLLTEEQDKFVDNRFSKALVRRLTLLDGRELDSFMQVFRPSFTFT
                     KIAGDYDFRLYIKQALYRFKHGLPPEAWFKEEEPVLE"
     gene            complement(14076..15008)
                     /locus_tag="PLU11_03655"
     CDS             complement(14076..15008)
                     /locus_tag="PLU11_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN58238.1"
                     /translation="MIKDKDKDNKEGSHKNIWYGAGRQRIEIPKTILKSRVNSNSMLR
                     HLHICSIGYYPKAKDHFTYRKKGLPENFLFYCVDGNGWFQIGKQRYEVGPNEFFILPQ
                     NIEHSYGSSTDHPWTIYWIHFGGEALPQFNEVQEIQKHFKPVYIKNNGEIIPIFSQIY
                     KTLELGYSVDNLLFANMCLSHFLTLFVYNGRHYAANSGDKLDCVDSAILFMQEKINDN
                     ISLNDLSAKYNYSISRFSNLFKQKTGYAPIDYFLQMKMQKACQQLNFSNRSIKDIAFG
                     LGFDDPYYFSKRFRKNIGMSPKKYRSLRSNDLND"
BASE COUNT         3859 a         3574 c         3523 g         3879 t
ORIGIN      
        1 cttctggaat ctcaccttcc cagttaccat gacccgggtt gatcggggtg gtccattcca
       61 gggtgttggc accccagggg ttggtagtcg ttacttttct tcccttgaaa atagaataga
      121 aaaaattgaa gacaaacatc agctgtaccg cgaatacaat gatcgaaacg accgtgatca
      181 ttttatcaag atcaccaaac tggttgaagg aaacccaacc tttcttatcc aggtaacggc
      241 ggggaacacc ggccagcccc tggtagtgca tgggccagaa gatcaggtaa gccccgatca
      301 tggttaccca gaaatgcagg taacctaatg tattgttcag gtagcggcca aacattttgg
      361 ggaaccagtg gtacacaccg gcaaacattc cgaagaagga tgccacaccc attacaatat
      421 ggaagtgcgc cacaacaaac atagtatcgt gcaggtgaat gtcaatagtc gagttaccca
      481 ggaagatacc ggtcagacca ccagagatga acaggctcac aaaaccgatt gcaaataaag
      541 cagccggggt gaagcggata tttcctctcc ataatgtggt aagccagtta aacactttga
      601 tcgcagaagg tacggcaatc aatagcgtca gcagcacaaa gaccgatccg aggaatggat
      661 taagtcccgt gacaaacatg tggtgcgccc ataccaggaa ggccaggatc gcaatagcga
      721 acatagaacc aagcatggcc atataaccaa agatcggttt acgggcactg acggataata
      781 tctccgacac catacccatt gcgggcagga ggatgatgta tacttcaggg tgacccagga
      841 accagaacaa gtgctggtag agaatcgcac taccgccttc gttcgggaga atttctccgt
      901 tgatcacaat atcactcagg tagaagcttg tacccaggtt acggtcaaat aacagcagga
      961 ttgcaccgga taataataca gggaaagaaa gtactcccag aaccgcggtg aagaacatgg
     1021 cccagatcgg cagcggcata cgggtcatac tcatcccttt tgtacgcata ttcaggatcg
     1081 tggaaatata gttcagaccg cccagcaggg atgatataat aaagagggcc atcgcgatca
     1141 gccagaggtc ggtaccaatt ttttgtccgt cacccgcctg acgcagggcg ctcagcggcg
     1201 gatacatggt ccagcctccg gaagcagggc cggtatgtac gaaaatggag gagagcataa
     1261 ctacgctggc caggaagaag aaccagtaag acagcatatt cataaaaggc gatgccatat
     1321 cacgggcgcc gatctgcagg ggaatgagta agttggcaaa tgtgccactc aaaccggcag
     1381 tcagtacgaa gaatacgagt acagtaccgt ggatggttac cagtccataa tagaattcag
     1441 gctgaatctg gccgcctttt gcccaatgcc cgaagaaggt ttcgagaatg gggaaagact
     1501 ggtcaggata acccaattgc aaacggaaca gaacagagaa cagtcctccg ataatggccc
     1561 atacgatacc ggtaatcagg aactgcttac cgatggtctt atggtccatg ctgaagatat
     1621 acttcgagat gaaggactgc tcatggtgac catgtccgtg atcatcatga tgtacagcgt
     1681 gtgctgctcc ggaatgtaca tgtaatgctt cgctcatatt ctcttttatt taagcaacgt
     1741 tgatgcttgt ttcaataatt aacggataat cgctccggtt acgggagcag cggcactcag
     1801 ggtgtcggct tttttagaag ggtccttatc aggattggct accaggtact gtggcttttt
     1861 gcctgccatc caggcatcaa actcttcctg ggtttccacg acaatcgtac ctctcatact
     1921 ccagtgacct ttaccgcaca tctggtcgca ggaaatctcg tatacgaaat cagggtttcc
     1981 tgttttctta cgcatatcgg cggtggtaat agtcggggta aaccacatgg ttgttggagt
     2041 accgggaaca gcatccattt tcaggcggaa atgggccaga cccacgtcat ggattacgtc
     2101 tttggcgccg atcaccagtt gtaccggttt accaactacc aggtgcattt cctgttctac
     2161 aaaaacgtcg tcgtaattgg cgggatcatc ccagatctga cctgccgggt tgttggcggc
     2221 cggatttaca ttcttgaaat attttttacc aaggacacca tctttacctg ggtagcgcat
     2281 ttcccaacca aactgcttgc ccacaatttc cacctgcatg gcattcttag gtgccgcacc
     2341 ggtgattttg aaccagtaga aaataccaac tcctaccagt atggtaagta caatggcagg
     2401 gattacggtc cagatcagct ctaacttatt attatgcggg aaataaaagg ctttttgatt
     2461 ttctttttcc tggtatttat aggagaacca gaaaagggct acctgggtga taacaaatac
     2521 aataaaggta atggctatcg taacatacaa catcctgtcc accagcacgc catgatcaga
     2581 agcaggctca ccgaggatct ttcccttcag ttgttcatta caatagtaaa cgccgatcag
     2641 tccaaggatc aggaaggcca gcaggagaaa gccgttgatc ttattggact gtttacggga
     2701 acgctcatat ccccttaata cagctacata ctcacttgct ttggcaatct ggaaggtgat
     2761 tacaaagcct aatgctaaaa ttgcgacaat gaaaaatgtc tgcataactt aaaatgaggt
     2821 ggtttttatc taatatctat ctaacaactt tacatcagag gcgaagcttg ttccggatac
     2881 catcaggtat ggtggatcag gctctctttt acaaacgggt ggtttttggc cagcaggggg
     2941 gctttgctca gcgcccttcc ggttacaaac ataatcagac ccacaaatcc aagcgcaaca
     3001 cccatgtcat ataaaatatt cggcacatgt gtggcgccag ttgaatcttt catcacactg
     3061 gggaatacca tctggtaaaa gtccagccag tgaccgaaaa tgatcatgat cgccataaat
     3121 gtaatagttc cgtagtttct tttggcatcc cgcgtcatta atattaacaa cggagcnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggg caatcagggc cataccggaa
     3301 acaaacgtac tggcgaatgt gtaccagctg tacatcgtac tgtaccagtg cgcatcgatg
     3361 ctcatcagcc agagccaggg aatggaagac attacggtca gggagaacac cacgataaaa
     3421 agggccgccc aaacggtatt attccagaca tatttcttac gtccttcaac agtagtgatc
     3481 ggattattat ccaggctgcg ggaaagctga cgcattttcc agcccagcac cgaccagagt
     3541 acaatggtca ggatcgtggc cactgcgaaa aaagtagtat tcaggaaacc ttttttgtgg
     3601 ctgaggatcg ggtcttcagc tacgtggtga gtatcagtcc agtggtaaat ggtatgatta
     3661 ccgccaaaag cgattgccag gaggatcgct ccggcaatta caccaattac aggaaccaca
     3721 gcggaaatcg cttcaggcac ccggcggaaa gccatttgcc agcctcccca tgccagggta
     3781 gtggcacaga taaagaacat ggaggcattt accaccagca aaaagaacac actgttttgc
     3841 agcagggaag cccagaacct ggccgactta tggggatcag aggaggatcc gctggtaacc
     3901 cacaatccag caatcgccag aatacccact gccatcaatg caatggcaat cgtattatat
     3961 ctctttggca cttcaaaatt ttcccggata gacatcagtt aatatttaat gtttcttcaa
     4021 ttcggtttgt tatttcatcg gggcaacggc cgcactgtct ttggcagccg gagccggagc
     4081 agcagtagca gccggagctg ctgttcctgt tttcttcgct tgttccgctt tcacataggc
     4141 cgtgatcatc cagcgctgtt tggtactgag ctgtgaagca tagcttccca tagtgttctt
     4201 accataagtc agggaatgga acatcgttcc ttctgtcatc gccaccatca ggggatcgcc
     4261 aaccaggtta cggggagctg caggaaatac accgctggta tataaaggtc cattaccatc
     4321 cagcttggta ccatgacaga taccacagtt aatcaggtac aggcgctcag cctctttcat
     4381 atcacgggat gccgtatcta acgggttttt aacttctgca gacttagcat agccgatcga
     4441 gtcgttctga taaggatagg ccgccatttc accacgggct atcgtaccgt ccaccggctt
     4501 agcggtataa tgtacaccgg ctttgtccag cgcttcagta gaagcatagg tttcataagc
     4561 ccggctgtag gacatatccg gcatataagc acggccttca tccgttctag gaccgccaca
     4621 actgatcatg gccaccatca gggctaaact accggtaaca aacagtattt tcttcattat
     4681 ctcttgttat tacgatgaat gatcttagtt ctccttgtga aacaatttct tgtccttatc
     4741 gtatcgacca atccaccact ctgtttcttt atattcaatt ttcacttctt tggcacctac
     4801 tgtttccagg aaactcagta cttcagcctc attggtctta tcggtacatt ccagggccat
     4861 cacgaatgtg tcgtccgtag aacggggatt aaaatgatcc ttttttacaa agggagccag
     4921 ctggcagagg taacagaagg tcagtaccat accgacggcc gcgaataata cggtcagctc
     4981 gaatgtaaca gggatccagg ccggcaggga gaaaaacggc ttaccaccga aattgatttg
     5041 ccagtctacc gtcagtgccc aggtaatgaa acccacagca gtggatgtac cagtgatacc
     5101 atagatgaaa cctgcggtgt gcagactggt atctcttaat cccatcgcat gatccagtcc
     5161 gtggatcggg aagggggtga atacatcatg gatcttgtat cctgcgcgac gtactttttt
     5221 cactgcggga aaaagtaccg cctcgtcatc aaaattgccg actacaaatt tttttacagc
     5281 cattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccttca atttcttcca
     5401 tatggtcttt gaaactctta ccgtttttct tcaggatatg cttgatctct gccagggcga
     5461 taaccgggaa gaatttagag aaaaggaagt aacaggtaaa gaacagaccg aatgtaccca
     5521 tgtagaaacc gatctcccat atagaaggtg tataataagt actccaggat gagggcaggt
     5581 aatcacgata aacagaggtt acgatgatca caaaacgctc aaaccacata ccgatattca
     5641 ccaggataga catgaagaaa gtcacaaaca ggtttcttct cattttccgg aaccagaaga
     5701 tctggggtga cagtacgtta caacccatca tcagccagta gctccagccg taaggactgt
     5761 tcaggttcaa ccggttttca aagaaggcga attcctcata gcttacggca gaataccagg
     5821 ccatgaacaa ctcagtgagg taggcgatac ccacgatcga accggtgagt acgattacct
     5881 tattcattac ttcaatatgc tccatcgtaa tgtagtcctc gagggccagc accttacggg
     5941 ttaccaccag cagggtctgt accatcgcga aaccggagaa gatcgcaccc gctacgaaat
     6001 agggcgggaa gatcgtcgta tgccatccgg gaacgataga ggtagcgaag tcgaaggata
     6061 caatcgtgtg tacagaaagt acgagcgggg tactcagacc ggccagtacc agggagaggg
     6121 cctcatgacg ctgccagtgc ttggtggaac cagtccagcc aaaagcggcc acgccgtaaa
     6181 aatgttttct ccattttgct ttagccctgt cacgtaaagt ggccaggtca ggcagcagac
     6241 cggagtacca gaataataag gaaacggtga agtacgtaga gatcgcgaat acgtcccaga
     6301 gcagcgggga gttgaagttc acccacaaag gaccacgggt attcgggtaa ggaagcacga
     6361 agaaggcgtt ccacacacga cccatgtgcc agatcggaaa ctgacccgcg cacattaccg
     6421 cgaagatggt catcgcttct gccgcacggt ttacccccgt tctccagccc tgacggaaca
     6481 gcaacaggat cgcggagatc agggttccgg cgtgaccgat acccacccac catacgaagt
     6541 tggtgatatc ccatccccaa ccgattgtct tgttcaggtt ccactgaccg gtaccataca
     6601 tcacttccat cgttacggag acgataccga agatgagtaa cgctacggaa atcagaaaac
     6661 ctacccacca cagtctggac gggctggctt ccaccgggcg gcaaatatct tccgttacct
     6721 ggtgataatc tttggtgcca tcgactaatg gtgctcttac ctgtgattcg tatctgagta
     6781 atgccatatt ctggttgttt gtccttccct ttttgagttc aggatatagt tagtcaactt
     6841 tcttcctgat taatgatgtg cttctgctgg ttttgtttca gcgggagcgt gtccttcagt
     6901 tttttctgca gccggtgctg catgatgtgc cggctcgatc agttcatccg tattccgcac
     6961 tttggccagg tagcttacat tcggcaatac gtggagttgc tccagtacat agaaactacg
     7021 gcttgggttt tctttccgca ctttagtgat ctcactgctg gtatcatgca catttccgaa
     7081 tacgatcgca ttggcggcgc aggcggtctg gcaggctgtt ttggcatcgc cgtctttcag
     7141 cggacgatcc tgttttttcg cttcgagttt agccgcttgt aagcgctgga agcagaagga
     7201 acatttttcg atcaccccgc gtgagcgaac cgtaacatcc gggttcagca ccatacgggt
     7261 aaggtcgtcg ttcatctgga atactgcggg atccagttta cctaccagtt gctgatcctg
     7321 gttatcgtcg aaactgtcag ctccggtata atccgcccag ttaaagcgac gaactttaaa
     7381 cggacagtta ttggcgcaat aacgggtacc gatacaacgg ttataggtca tctggttcac
     7441 cccttccgca ctgtggttgg tggcggctac cggacaaacg ttctcacaag gagcgttgtc
     7501 acaatgctga cacatcatcg gctggaagac cacgcctttc agctggtcag ggttcttttc
     7561 atcgcttaca aaataacggt cgatccgcag ccagtgcata tcatggtaac ggagcacttc
     7621 gctcttaccc accaccggta cgttgttttc tgcatgacag gccaccacgc aggcgccgca
     7681 gccataacag gcattcatgt cgatgttcat gccccatttg atacccggct gatcgtgcat
     7741 cgggtaaagg gttgcttctt tacggtaatc cttggtcttg ccatccttgc tggtaccgaa
     7801 gtcttcttcc aggtgattgc ggtaatcagg aatcacggtg gggtatttct tcagggtggc
     7861 cagggtggtt tcgcggacta cttcaatccg gtcttcgtat gaattgtgaa tctgtgtttg
     7921 tgcaattttc tctttccgtc cggcatcagc agctaccacc gcattgtgat aaaccacggt
     7981 agagccatta taagaagaga atggataaac gtttttacct acgccggcag ctgttttacc
     8041 caggttctca ctacggccat aaccaaccgc caccgcaatg gtgttggcat ccataccggg
     8101 gatgaccagt acaggtaatt ctatatctac cggcttgttg ttggtgttaa caccaacaac
     8161 agtcagcttg atcaccggct tctgaggaaa atactcgtag ttattacttt ctttctcact
     8221 gccatcgatc aggttcagac ccagcagtga tttggccatg gtcaggctga tcagtgcata
     8281 gttgtcccag gtggctttgg tgataccatc cggtaattcc tgcagccagg ggttggtggc
     8341 accggttccg gtaccaatcg atgttttttg atagagcact acttcggtac ctgtgccttt
     8401 tttggcagct gcgattgcag cactggcttc gattacagca actccattgt atgcaccagc
     8461 accggcagct gtcaaaggct cttctttcac cccaatttgc aggatgctgt tgaatacatc
     8521 ttcagcaccc agcttggtcg cccaatagtt cttgaaataa gtttcgtact cagtattgtt
     8581 accactccat ttcagcaggg aagtctggaa cggacgtgtt ttgaataaag gataaatggt
     8641 aggttgttgg aaactgactg cacctgtttt agcttccgca tcaccccagc tttcgaggta
     8701 gtggtgagtg ggcaggatga aatcgcagag ttcagtggtc tcatccattc tttcattgaa
     8761 ggatactttc actttcactt tggccagagc cgctgtgaaa cgggccgcat ctgttgatgt
     8821 ataagcgggg ttggcaccgt agatcatcag ggttccaact gaaccagctt ccatatcggc
     8881 gagcagttga gcaaaatctg aatcaatacc ctggcgataa ttcaccggct tgctccagtc
     8941 gatggtttta ccaaatgcgc caatggcatt gttgattgcg ttcacgatca cctgtacatt
     9001 cttatcgttg ctgccactta ccaccagggc tgtacccatg ttggctttca ggtcttcggc
     9061 tacttttttg ataccgggac gtgtgctggc ttcgccggtc agggcgttca gcagctccag
     9121 ggccacggct ccttcttcag aaggacgatg agtgaaacgc tcatcggcac tggctcctgt
     9181 caggctcagg aagctctcga actggtagtg cttgctcatg gcaaaatgct gctcatcgat
     9241 cttacgaccg gcggcataac cctttgcatt ttctgctgca ttcagccagg taccgagaaa
     9301 atccgcaccc agactcacga ttactttggc tgcaccaaaa tcataagtag ggatcttacg
     9361 gccaaatccg ctggcttcgt tggccagcag cataccgctg taagaaaccg gatccacctg
     9421 cacatgtttc aggccgggaa acgcggcaat aatttctttc gtagaaggag aaacgatcgt
     9481 gctggtcagt aacaccgtgg tgccgccagc cgctttcatt gcctcagcta ccagtttatc
     9541 gaactgttcg aacgtaggaa cttcattgta tttggccgct tcacctgttc cggttttctg
     9601 gatcggaaaa cgaagacggc tggtatcata cagatcaagt acggaagcct gtacgcgggc
     9661 cgttgtgcca ccgccggtat aaccacaatc ggtattccct tcaatcttga taggacggcc
     9721 atctcttact ttagccagta ccggtaccac atctccatcc tgtacataag tggtggcata
     9781 atactgtgcc acaccgggaa tcaggttttc gggtttgttg gcgtacggaa tcgctttctt
     9841 taccgggatc ttacagctgg cagccaatga ggcggcggcg gtgctgaaac ccaggtactt
     9901 caagaaatcc ctgcgggggg ctttggcgtc tattaatcct ttgtcatcga aaccttcaaa
     9961 aggtaattcc tcacgaaatt cgtcctgctg ctttttctga aagttttcgc tgtcattcac
    10021 ctctccgaaa ctttgccagt actttttttg gctcatgaat atcagtttga taattcgata
    10081 atgtgctgaa tacaattgtt gacgttacgg acctgcttag tagtgacatt tctgacactc
    10141 aagaccaccg atatctttca ctttcacgct gtccattttt ccggacttga tatcattgtg
    10201 gaacttctca tagatgctgt aaaacttgtt acccttggtg ctgtcgtagt tgaagttcac
    10261 attggtctga cggtgacagt tgatacacca tcccatgctc aggtcggcca cttgttttac
    10321 ttcatccata gcggtgatct caccatgaca gctctgacac tgtacattac cggcacggat
    10381 gtgctgggag tggttgaaat aaacgtggtc tggcagatta tggatcttag tccattcgat
    10441 tggtttcgct ttagcaggat cccatttgga aggattgttc ggatcgaaac cggcaaattg
    10501 ataaagcttg gcgatctcgg ctgtaccgtt tacttcatcc ccgttttctt tatacagttt
    10561 gggtcctttt tcgtaggtgt tgatggcctt gtggcagttc atacacacat tcacagatgg
    10621 aatggcggca tgcttgcttt cccaggcgtt tccatggcag tacagacagc tgatctggtt
    10681 gataccggcg tgtactttat gggagtaata gataggttgt tccggctgat agtctttctg
    10741 acgacccagt ccgatggcgc ctttggcgac catataacca ccgaatacaa agaatacgat
    10801 ggaggccatg gcgatatagg tcttgttgcg gtagaaggct acaggctcag gacgaacgat
    10861 tccttcagaa tcatccgaca ttttcttcag gttgctattt acctgcatca ggatcaaggc
    10921 aatgataccc agtacaaggg agatgacccc gaaaatgatc gcattctggt tgccggtatc
    10981 tgcagtttca gtaacagcgc cgggtttagg agctgcattt ttatttgcca cttctgcatt
    11041 gatataggct accatatcat ctacatcctt ctgggtgaca tcagcaaaac cggtcatcat
    11101 ggagccgtat tccgccttca gtccctgggt atagggatcc ttggccatat aggctgccgg
    11161 attgttgatc caggccagca actccttatg gtctgcccat ttaccacgtt cttccagtcc
    11221 catcagggcg ggacccgtca tctttttgaa gacgttgtgg caggcggcac actttccggt
    11281 aaagatggct ttaccggcag ctgcatcctg tgcagaaata ttaactatcg aaataacaaa
    11341 tagagataga aatagtacag tcagtctctg gccgattggt ttacaaagaa tcatagatgc
    11401 aattgatctg aaaaatgtgg ataaatagct ctaatcaggc gcaaaaataa gtcagtatca
    11461 ctataaaaag ccaatagctt taactttttt ttgatccgct accagcctgc gtttcagccg
    11521 attttcagtc ttttttttga agggtcaaac tctgtttgtc ataaatcagt aaagcctgac
    11581 ctaaagttca acatatgtgg acgaagcggc atttattggt tgttttttgt cgacttttgc
    11641 tccgcattat tcaaaaagca cgacatgttt gagggtttac aacagaaatg gaaggtgaaa
    11701 ggcggtcagc tggccctgat tctctgcacg tttgccattg gtggaagtgc aaccggatgg
    11761 gtggccaaga agattatgaa cgtattagcc attgagcagg actggctctg ggctgttata
    11821 tatattatac tggttacgat ttgctggccg ctgatggtca tcctgatcag tattcctttt
    11881 ggtcagtttc ccttcttttc ccgctatatt aaaaaactgg gcagccggat cggtctggtc
    11941 agatcaaaga cgcctgatat caatgagtga aaaactgacc catatcgcca tttttgcctc
    12001 cggggctggc agcaatgcgc atcagatcat tcgtcatttt accgggtccg caaaggcgaa
    12061 aatcagcctg attgtatgta ataagcctgg ggctggaatt ttacaaatag ctgaaaaaga
    12121 acatattccg actcttttaa tcgaaaaaga actctttttt cagggagatg cgtatgtccc
    12181 tgaactccaa aaaaagggga tcgatctgat cattctggct ggctttctct ggaaaatccc
    12241 gccggccctg atccgggcct acccggaccg gattgtcaat attcaccctg cccttctgcc
    12301 caaatatggg ggcaaaggga tgtatggccg ccatgtgcat caggcagtaa ttgaaaacaa
    12361 agaagccgta agcggcattt cgattcattt tgtggatgag ctatacgaca atggcgctgt
    12421 catatttcaa gccagttgtc cggtactgga aggggatact cctgagagcc tggccaaaag
    12481 ggttcaggaa ctggagcatg cccattttcc gaaagtgatt gaagggctgc tgtaatatac
    12541 cttcaagggg gtatagttct gtacgcggtt taactgcatc tttatctctc aaacaaaccc
    12601 tttatgaaaa agctgaccct gtttttcctc ctttttgcaa gtaccctgac cagtaccgca
    12661 cagaataacc cgatcctgat caccccgggc gtaggcgtta ataatattaa attgggcatg
    12721 tctgaaaaac aggtgaccga tctcctaaaa ggtgaaccgg tgggttatac ctatgcagat
    12781 cagttggatg ctttcaaagg atatgatacc cggatcgata gtgtgatgca atttgtgctg
    12841 ggatttgaca gttgtatccg ctatgataac gaactgccgg tcagcatgcc ggtattcgga
    12901 ttatatttca aaaatcacaa actgaatttc atcaccgtca cttcctattc cgccacggaa
    12961 gatcacctga aacaggttcg tctcaataac gggcttaaat tccacgacag catggaggat
    13021 tgcggaaaaa aactggcgaa atcccctttt gtagccctcg gatacggcga ttattccggt
    13081 gatcattatt attataccct cggactcgaa atggtatacg acgaggataa gcttactgct
    13141 atcggcatct accccattac ccgtgatttt ccggccaaaa tcgctgaaaa cagcgaacgc
    13201 ctgctcaaag aagccagtgc agaaggggaa gaagaaggaa ataattgatc aagtccttac
    13261 actttagaat agcttttgca ctttctttcg ttacttgctg ccggattgaa aaagctatac
    13321 ccttatatta tcatttccct gctctttttg ggagcagctt acaacagcca ggctcagacg
    13381 taccggatta aagggtatgt gctggacagt tcccgcaatt acccgatgga actggtgagt
    13441 gtaatctcca gttcaggcaa gggaacggtc accaacagta tcggttacta tgaattagag
    13501 gtaagtgaac aggatacgat ctggttcagc tacctcaaca agtccaccat gaagttcccg
    13561 gtggctaaga tcagcaaccc catgcagttt gatatttcca tacaggtaaa tgtacccacc
    13621 ctgaaagaag tgaaaatttt accccgcaat taccggcagg attcgatcca gaaccggctg
    13681 gactatgcca aggtattcgg cttccagaaa ccaaaactca aaacagtcac gcctacttat
    13741 ggagccggtg taggattcga tctcgatgag atcatcaata tgttccgctt ccggcgcaac
    13801 cgcagcatgc tttctttcca gcaacggctg ctgacagaag aacaggataa atttgtagac
    13861 aaccgcttta gtaaagcact ggttcgccgg cttacgttgc tcgacggtcg tgaactggat
    13921 agttttatgc aggtattccg gccctcattt acatttacta aaatagccgg cgattatgat
    13981 ttccggttgt atatcaaaca agccctctac cgtttcaaac acggattacc tcccgaagcc
    14041 tggtttaagg aagaagaacc ggtgctggaa taaaatcaat cattgagatc attagaacgc
    14101 agtgaccggt atttcttcgg cgacataccg atatttttcc tgaagcgttt ggagaaataa
    14161 tagggatcat caaagcccag tccgaaggcg atatctttta tcgagcggtt gctgaagttg
    14221 agttgctgac aggctttctg cattttcatc tgcaaaaaat aatcgatcgg tgcataacct
    14281 gtcttctgtt tgaacaggtt ggaaaaacgt gagatagaat aattgtattt cgcactgaga
    14341 tcattcagtg aaatattatc attgatcttt tcctgcatga agagaatggc actgtccacg
    14401 caatcgagtt tatccccgga gttggccgca taatgccggc cattatacac aaacagggtc
    14461 aggaaatgcg agagacacat attcgcgaac agcaggttat cgacgctgta acccagttcc
    14521 agtgttttat aaatctggga aaagatcggg atgatctccc cattgttctt tatatagacc
    14581 ggtttgaagt gcttttgtat ttcctgcact tcattgaact gtggcagggc ttctcctccg
    14641 aaatgaatcc agtagatggt ccagggatgg tcggtactgc tgccatagga atgttcgatg
    14701 ttttgcggca ggataaagaa ttcattcggc cctacttcat aacgttgttt cccgatctgg
    14761 aaccagccat tgccatccac acagtaaaac agaaaatttt caggtaatcc tttcttccgg
    14821 taagtaaaat gatctttggc cttgggataa tagccaatcg aacagatatg caggtggcgc
    14881 agcatggaat tgctgttgac cctggacttc aggatggtct tgggaatctc aatccgttgc
    14941 cggccggccc cgtaccagat gtttttatgt gaaccttctt tgttgtcctt gtctttgtcc
    15001 ttgatcataa gtggcgtatc gggagctaaa gtaag
//
LOCUS       DAOPMW010000040        14962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4005_length_14962_cov_8.022741, whole genome shotgun sequence.
ACCESSION   DAOPMW010000040 DAOPMW010000000
VERSION     DAOPMW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4005_length_14962_cov_8.022741"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            823..1371
                     /locus_tag="PLU11_03660"
     CDS             823..1371
                     /locus_tag="PLU11_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58239.1"
                     /translation="MKFDKTFWTTTFLSIAIMLLTDIASGQSVIDTPKKSKTNKSLDT
                     ECHLTDFERQNIFTKVEVLATFKGFSPEWFKYAQNNFDFNSVSRKLSDTAQTFHDSIF
                     LRFIVTKVGLLCKIVVLKGNNILSDPAIKLIKQSSPWTPAVSGGRNLNVYRTLKIEVY
                     IDKQKNEFKIIRDFNSYSNPDG"
     gene            1572..2354
                     /locus_tag="PLU11_03665"
     CDS             1572..2354
                     /locus_tag="PLU11_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58240.1"
                     /translation="MKRHFLYFIYFLFCLSIVVSSQSNFPAFENWKYSKVPTNPDTIY
                     SYNLDNSNWIVYRKNDNIRVKEMMDISDREYLPFKTPTPISYSKMEEFTGKRSFIKVT
                     DGYLVAFWRGEFGASLFWFSDDGTRYYKINGASAVQFIERNGEIYAINGLAHLDMSNG
                     ILFKIDRENNKWVANKYLELPFAPYAVSLDSNNDFIVVTSDNLIKINRKKRIKTLLKT
                     DFWQGLYPTSSVIEGEYCYIGMRKGVYKFNLRTKDSDWLLPK"
     gene            2540..3061
                     /locus_tag="PLU11_03670"
     CDS             2540..3061
                     /locus_tag="PLU11_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58241.1"
                     /translation="MTKKILISILSLLTAYYTIAQTNNPKILVDSLKHLKEDTIDCNT
                     DLYWRIIAIGKDAIPPLIDKLTDTSLTNVRWQCKKEKLNVGEIAQLALVEIAYFPAAK
                     ITQWQFDIIENGCWNFYDYFFNNKNKVHYQNLIKVWYQEQFQNYRAIEIPQKKLSACR
                     LKYNLIKYYEWQE"
     gene            3298..3906
                     /locus_tag="PLU11_03675"
     CDS             3298..3906
                     /locus_tag="PLU11_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58242.1"
                     /translation="MKLIFILLFSACYYSLFGQAVSLQEYFPLKNGVGRLFYVSHISG
                     TDTLQTDNDSSICKSLLIKGKEIFYFDDNLFSDDNTIIGSNSFCDGVFYYENGNFIFS
                     PLFWKYELKEANLDYFETLFPAKISFDTIYKYQDGEEKRKYTFIGFEDVYIKDKLYKD
                     CLKLIITQDWKTAQYIDTVWFFKGLGVVKWLRGTGRLEEIKL"
     gene            complement(4127..5002)
                     /locus_tag="PLU11_03680"
     CDS             complement(4127..5002)
                     /locus_tag="PLU11_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN58243.1"
                     /translation="MDNFAPMKPFMLFGSSVMIVVVLLAALAFINTKNEIPEKHVEVV
                     LRTIGHQLLLSAKDSSSRVLPVKKTAENIYQISFQHEVGFLSDSLINIVHRTFQKTAL
                     AANYIVNLRNCDQKETVLAFEMNSKTGDLTPCRGRTLELGCYMIEIELLRANTFNFRW
                     LWLLIIPLIVAGFYLKEKFRKKEPKESVSDSTDYIQVGIFQLYTAKNVLKSGHKNITL
                     SENETKALEIFAQDINQVVEREKLIKEIWEDKGLVVISRNVDVLVSKLRKKLTDDSSI
                     KIVNVPGRGYKFIIE"
     gene            5131..6123
                     /locus_tag="PLU11_03685"
     CDS             5131..6123
                     /locus_tag="PLU11_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58244.1"
                     /translation="MKFIYPLAVLLIFPFLSCNQASQYPQTAQLTGSLPLQNVDKTGL
                     VFKSIDGGQSWLNISEGLPGDKLEGSLFVNDLGFHLRTGKYIYHSKPSSKAPFWEKEI
                     FPDEHSNIAPGKSGMFAYDYHQGLFRKKLNGSREWSPVFENFELKGIIDVFETNGGTV
                     FIICDRGRGLFRSTDNGKNWKEVSRGVINLTASNGVLMANSINGVIRSADDGETWETV
                     ISEGGVGIDIAPIDGGFAAITFNTQSETRRVRASYDGGKTWQAIDAGLPPSKNISSII
                     QVGDSFFCGHPNGIYQSGDKGKTWKLILPSIEGKVFSLSVSGNVIYAIPSNGGC"
     gene            complement(6151..7122)
                     /locus_tag="PLU11_03690"
     CDS             complement(6151..7122)
                     /locus_tag="PLU11_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPN58245.1"
                     /translation="MKKPPVSILAVLLATLLVASCKKDKTPAPVAALRFTKTTTAGSH
                     FAPLFDHQTVVFNNKLWVIGGRKSDILYTNEIWSSTDGAAWAQTTVSGPVFSVRSNHQ
                     VVVFNNKLWVIGGLSNGSALNDVWNSSDGAAWTQVTPNAAFSIRSLHDVIVFNNKMWL
                     IGGDGIGGTKNDIWSSTDGITWTQETVTGTQFGPRAGHQVVVFNSKLYVIGGYTATGF
                     KNDVWSSTDGRNWVEEIASGSKFVPLYSHQAIVYNNALYVMGGLVGFVTTDEIWSSTN
                     GKDWTKETNAAFGTRGGHQAVIFNNKICVTGGYDAAAVIKNDVWFSN"
     gene            7746..8852
                     /locus_tag="PLU11_03695"
     CDS             7746..8852
                     /locus_tag="PLU11_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58246.1"
                     /translation="MIAGNHTTDGTGTSTFSSLLNGLNPGTTYYVRAYATNSAGTGYG
                     NEVSFTTTTPAVYTAGYEYNTNSVCVAKVWKNGVETALSSGFSNAQGWVRAVFVSGAD
                     VYVAGYEFNGIKNSAKLWKNGTTTALSDLTNDSQAWAISVSGADVYAAGYEKNIAGIS
                     VAKIWKNGAPLSITDGTNDAQVYGIHIAQADVYAAGFENTSGVSTAKLWKNGAVVPLQ
                     GAGTNTTTYSVYVSGTNVYVAGSEIIPGGYSRAILWTNGVPAYLTSGIGSIAIPYSVY
                     AYGTDVYVAGFDQSTGTGLSKATLWKNGIASSLTNGSASARANSVYVYNNDVYVTGYE
                     LVWPFSISRTWKNGTVIHSTNGSFNAATTSVFVQ"
     gene            9085..10218
                     /locus_tag="PLU11_03700"
     CDS             9085..10218
                     /locus_tag="PLU11_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58247.1"
                     /translation="MKAISATLLMLVLAFLSLFAQAQKGSSIVIHDNTSPANGGENAT
                     SALRKEIASAFNREKPCVDLMDDEDIRNIIESEREKDLLEGADPQQVLTDIANLMGAS
                     YAMSVSSTPGPGGSTSYTVIVMDPQTAKTIARQTGSDPKQMADNIVKQLGSSLPDDCQ
                     PHWVGEVKYEYSSNETKTTNDAGAMHASTRNTKRSSTVTYSAQNNIVATLLPPKPGSP
                     VSSGKTMARVWMRSKIIGELKQETAGEVYCRLKGQNPVWKGYNLNYSETTTQLGGGAD
                     NLPVSISIDSEGNYKIFVKTPGGTLLGKFETNRSESGCTTDNPPVKSAVSMPEQKLDA
                     SGFDVTGKTDAKNRDSLSGSKTLPDGKTKITWNLKLVKPKSKK"
     gene            10377..11255
                     /locus_tag="PLU11_03705"
     CDS             10377..11255
                     /locus_tag="PLU11_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN58248.1"
                     /translation="MVNFQERVLHYPQYCKQFSCGDSLITIFNCPPEARLMKEKFADL
                     WSHENYIFFVLEGKKVWHTADGSYEISEGSCVLVRKGAWILEQFFDIGFCLVLFFIPD
                     QFICETLKSRSAPIHHTGKNYNSVIPIVATETLKSFFVSIYAYFAGTTSPDPSLLELK
                     FRELILNIADNRDNAELLSYFCSIMNEPQAVSLERVMHDNYCFNLKMEQYAALCNRSL
                     SAFKRDFQKQFNSTPGKWLLEKRLQHSMTLLKNTGKTVAEAAFESGFENNSHFSRCFK
                     QRFGLTPMVAKQSMVS"
     gene            11365..12183
                     /locus_tag="PLU11_03710"
     CDS             11365..12183
                     /locus_tag="PLU11_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN58249.1"
                     /translation="MNPNKALWEKGDFTKIADTMRESSAALVATLGIQPGIAVLDVGC
                     GDGDTAVPAAKLGAQVTGLDIAHNLVEAGNLRANKEGVAASCKILEGDVMDMKELKDA
                     NYDLVISIFGAMFAPRPLDTAKEMVRVTRKGGRIVMGNWIPGDPTLVAQILKISSAYT
                     PAPPEGFISPMRWGVEEDVISRFGNAGIAKENISFSRETFTFQADYAPSVLVNNFKNY
                     YGPTMNAFEAAEKTGNAGALQLELENLFNSQNQSGIGNRTSIPATYLKVTVRCP"
     gene            12296..12724
                     /locus_tag="PLU11_03715"
     CDS             12296..12724
                     /locus_tag="PLU11_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58250.1"
                     /translation="MKKYLLFCLLVLSLKSYSQCTECNSVHLALKKPGQVRSLILNGH
                     QHGVVMDSIPVAIGQLVNCEILYLTDHRIKKVPATIGKLQKLKELSFGGCQLERLPEE
                     IFSLKNLKELILFDNAFSEEYIKELRQRVATALPKTKLML"
     gene            12764..12886
                     /locus_tag="PLU11_03720"
     CDS             12764..12886
                     /locus_tag="PLU11_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58251.1"
                     /translation="MPEYEKFQACYEQEGDKLFLQFNQFTEEQLLSENWKNALS"
     gene            12940..13671
                     /gene="bla"
                     /locus_tag="PLU11_03725"
     CDS             12940..13671
                     /gene="bla"
                     /locus_tag="PLU11_03725"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaB/IND/MUS family subclass B1
                     metallo-beta-lactamase"
                     /protein_id="HPN58252.1"
                     /translation="MRIILPLLSLFINLVNSHAQNSAPKLKITPLTGDFYIYTTYNTY
                     EDSQVPANGMYLVTDNGVVLFDTPWDTTQFQPLLDSIKLKHNKKVNLCIATHWHSDKT
                     AGLEYYRQQGIRTYTTKLTDEWSHKNNMPRAEFLMYSDTVFQAGQYRFETYYPGEGHT
                     ADNIVIWFNKEKILYGGCLIKGVSDVSLGYLGDANSIAYASTLKNVRKRCRKPRSIII
                     AHNDWNNLNSLKHSIKMAKRLKRKQ"
     gene            13929..14648
                     /locus_tag="PLU11_03730"
     CDS             13929..14648
                     /locus_tag="PLU11_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58253.1"
                     /translation="MKELEDYSWFPSVLRKFQTEFIGFVVARFNVYDGFVKHLNTLSL
                     PAQPLTDLCSGSGEPAIHIFKKSHCFTHLFLSDKYPASQVVKSGEDSYAMARKDVLEM
                     EFNPGTCYSMFNAFHHFSDEDQLRIIRKLHHSGSTAFLAEILEPDIICFLKVLFTTTI
                     GCLLLTPFIRPFSFRRLFFTYLLPVNILTITYDGLVSVLKSRTANHYRKLFADQGEGI
                     EVIRLRNGLTPVIIIQIHAGT"
     gene            14645..>14962
                     /locus_tag="PLU11_03735"
     CDS             14645..>14962
                     /locus_tag="PLU11_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPN58254.1"
                     /translation="MKTLLTIWKFSRPHTIIGSVISIITLYFIVCDKKEVQYLSFLVP
                     ALIIGITCNIFIVGINQIADIQLDKINKPNLPLPSGALSPAQAWLIVSIALSISLGLA
                     LFIT"
BASE COUNT         4477 a         3294 c         3102 g         4089 t
ORIGIN      
        1 atctaaaaac aactttaact caggaacaac ttttgcagca cggctgccag gaaccatgcc
       61 tgtcggcagg caggctttga tcggccagtg tacaaaaccc catatgttag cagccttaaa
      121 tcagtaatta ttaaatgcga ttttatttaa tattaatact tagtttcttt gtggtaaact
      181 gcatcactag tacacagaaa attgtagata gaaatactat aacatttaat cttttccata
      241 cgaacgacac tataaacctt aatgtctcct atttttctaa aaacaatgcc tcttgtggtg
      301 tacatgcaat accctatgca ttactgattg gtcaaacttc aactacatgt tcaagtaata
      361 ttccaaagac tattacagta ttggcctggt gtgacactca agattataga gttggacaga
      421 atattaaagt aatagtcatt caagacccga ctacccgaac aggtttacaa ccactttata
      481 aaatcaagga cactttaatc cacaatcaaa aacataaatg ggttttgggg tcagaatatc
      541 ctgctatatg gggaaaggtg ttgtaaatta ttcaactgct gcaaacatcg ggttttgtgc
      601 aatggccatc agaatatatt ctcaacgaca gaataaaacc cagcaacata tcgggctggc
      661 acggccttct agctatttgc tctaaaaaac gataattact ccaggaacgg atttgtgcgg
      721 cccggctaca aggaaccttg cctgtcggca ggcaggctcc gatcggacac agcacaaagc
      781 cccatccgtt gtgcgctatt ttaaaccgac attctattct caatgaagtt tgacaaaaca
      841 ttttggacaa caacgtttct atcaatagca atcatgcttt tgacagacat cgcttcagga
      901 caaagtgtta tagacacacc aaaaaaaagt aagaccaata aatcattgga cacagaatgt
      961 catttgacag attttgaacg acaaaatatt tttacaaaag ttgaggtatt agctacattc
     1021 aaagggtttt caccagaatg gtttaagtat gcacaaaaca attttgattt caattcagtt
     1081 agccgaaagc tttctgacac agcacaaact tttcatgact ccattttttt aagatttata
     1141 gtaacaaagg ttggactttt atgcaagatt gtagtactta aaggaaacaa catcttgtct
     1201 gaccccgcaa ttaaattgat aaagcaatct tcaccttgga cacctgcagt aagtggtgga
     1261 cgaaatctaa acgtttacag aacattaaaa attgaagtgt atattgacaa acaaaaaaat
     1321 gaattcaaaa taatacgaga cttcaattca tattcaaacc cagacggttg acgaatgcaa
     1381 aacaaacgta caaaatggag ctttgtgtaa tggctatcgg attatattct caaaaacaga
     1441 attaagccca gcgacatatc gggctggcgc ggccttctag atatatgatc taaaaaaaga
     1501 aactttaccc aggaacggct tttgaggccc ggatcggcca ttgtacaaag cgctatacgt
     1561 tagcagcaac tatgaaaaga catttcctat actttatcta tttccttttt tgcctgtcta
     1621 ttgtcgtttc ttcgcaatct aattttcctg ctttcgaaaa ctggaaatat tctaaggtgc
     1681 caaccaatcc agatacgatt tactcatata atcttgataa tagcaattgg attgtttata
     1741 ggaagaatga caatattcgt gtcaaagaaa tgatggacat ttcagatcgg gaatacctac
     1801 cctttaaaac tcctacacca atatcttaca gtaaaatgga agagttcaca ggtaaaaggt
     1861 cttttattaa ggtgaccgat ggctatttgg tcgcattttg gcggggagaa tttggcgcaa
     1921 gtctattttg gttttccgat gacggaacaa ggtattacaa aataaatggc gcaagtgctg
     1981 tgcaattcat agaaagaaat ggagagattt atgctatcaa tggtttagct catttggata
     2041 tgtcaaatgg catactgttc aaaattgatc gtgagaataa caaatgggtg gcaaataaat
     2101 accttgaact accatttgcc ccatatgctg tatcattaga ctcaaataat gattttatcg
     2161 tagtcacttc tgacaattta ataaagatta accgtaagaa acggataaag actcttttga
     2221 aaactgattt ttggcaaggt ttatatccaa ccagttcggt tatcgaaggg gaatattgtt
     2281 atataggcat gaggaaaggt gtttataaat tcaatctccg gacaaaagat agtgactggc
     2341 ttttgccaaa atagctgctg ctaaaatggg gtttggggtt ctggcggctt tatgagcata
     2401 tcttcatcta tattacattt ctcagcgaca tatccgggct gacgtaacac ggaatatcgc
     2461 cacgacggca agccccatac gttgtatgca aggtagtaag atatagtact gctggaattt
     2521 cccaatccat ttgtgttaaa tgacaaagaa aatattgata tcgatacttt cattattaac
     2581 agcctattat acaatagcac aaacaaataa tccaaagatt ttggttgaca gtctaaaaca
     2641 cctaaaagaa gacacaattg attgtaacac agatttatat tggagaataa ttgccatagg
     2701 caaagacgca atcccgccgt taattgacaa attaactgat acctctttaa ccaatgtcag
     2761 atggcaatgt aaaaaagaga aattaaacgt tggtgaaata gctcaattag ctcttgtaga
     2821 aatcgcctac ttcccggcag caaaaataac acagtggcaa tttgatataa ttgaaaatgg
     2881 atgctggaat ttttatgact attttttcaa taataagaat aaagttcatt atcaaaatct
     2941 gatcaaagtc tggtaccaag aacaatttca aaattacagg gcgatagaaa ttccacaaaa
     3001 gaaactctct gcctgcaggt taaaatacaa tcttataaag tattacgagt ggcaggaata
     3061 ataccctgca tacaatatcg ggttttgtgc aatgaccatc agaatatatg ttcatcaaca
     3121 gaataaaacc cagcggctgt tcgggctgtc aaggcctatt agatatatgc tctataaaac
     3181 gaaatttaag ccaggtacgg ctttttgcgg cccggctgcc aggaagcctg tcggcaggca
     3241 ggctccgatc gacaactaca caagcccgat acgttagctg caaatattca gagtccaatg
     3301 aaactaatat tcatactttt attttccgcc tgctattact ctctatttgg acaagcagtc
     3361 tctttgcaag aatacttccc attaaaaaat ggcgtaggaa ggctctttta tgttagtcat
     3421 atatctggca ctgatacact tcaaactgac aatgactcat caatctgcaa atccctttta
     3481 attaaaggga aagagatttt ctattttgat gacaacttat tcagcgacga caatacgatc
     3541 atcggctcaa attctttttg tgatggtgta ttctattatg agaatggaaa ttttatcttc
     3601 tcccctcttt tttggaagta tgaattaaag gaggcaaacc ttgattattt tgagacattg
     3661 ttcccggcta aaatttcatt tgacacaatt tataaatatc aagacggcga agaaaaaaga
     3721 aaatatacct tcattggctt tgaagacgta tatataaaag ataagctata taaagactgc
     3781 ctgaaattga taatcacaca ggattggaaa accgctcaat atattgacac cgtttggttc
     3841 tttaagggtc ttggtgtagt aaaatggcta agaggcacgg gcagacttga agaaataaag
     3901 ttatagctgc agctcacatc gggttttgtg caatggccat cagaataaaa cccagcgaca
     3961 gctcgggctg gcaaggcctc ttaaaaaact gatctaaaaa atcttttccc ccggaactgc
     4021 tttttgcggc ccggctgcca ggatccatgc ttttcggcag tcagatacgg accaaccact
     4081 gcacaaagtc ccatacgtaa tagtcaaact gatttttagc cgacccctac tcaataataa
     4141 acttataccc cctgccgggc acattaacaa ttttaatgga ggaatcgtct gtcagtttct
     4201 tgcgtagttt agacactaac acatcgacat ttctgctgat gaccaccagg cctttatctt
     4261 cccaaatctc ttttatcagt ttttcccgtt ccacgacctg gtttatatct tgggcaaata
     4321 tttctaacgc ttttgtttca ttttcagaca gggtgatgtt tttatgcccg cttttaagca
     4381 catttttagc agtatacaat tgaaagattc ctacttgtat ataatcagta ctatcggaga
     4441 ctgattcctt cggttctttt ttccgaaact tctctttcag ataaaatccg gcaacaatca
     4501 acgggataat caacagccac aaccaacgga aattaaacgt attggccctc aaaagttcaa
     4561 tttcaatcat ataacaaccc agctccagcg tacgccccct gcagggtgtt aaatcgcctg
     4621 ttttactatt catttcaaat gccaggaccg tttccttttg gtcacaattc ctcaaattca
     4681 cgatataatt ggctgccagc gcagtttttt gaaatgtccg atgaacgata ttaataaggc
     4741 tatcggaaag gaaaccaact tcatgctgaa aggagatctg gtagatgttt tcagctgttt
     4801 ttttaactgg cagcacccgg gatgaagaat cttttgcgga aagcaaaagc tgatgcccga
     4861 tagtacgtaa taccacttct acatgcttct cagggatttc gttctttgta ttgataaatg
     4921 ctaaagcagc aagcaaaaca acgactatca tcactgaaga tccgaacagc ataaatggct
     4981 tcataggtgc aaaattatcc ataaaacccc ggttttgagt ggttttacaa gcttttacac
     5041 ctcattttac aactctttac cgttaaaaag ccttcccgta gctggcgacc gatatcttta
     5101 ctttatcgtt tcacccaatc aagtatagcc atgaaattta tttaccccct tgcggttttg
     5161 ctcattttcc ctttcctgtc ctgcaaccag gcgtcccaat atccacaaac agctcagctc
     5221 acgggtagtc tgccgcttca aaacgttgat aagaccggcc tcgtgtttaa atctattgat
     5281 ggcggacaaa gctggctaaa catcagcgaa ggactgccgg gagataagct ggagggtagc
     5341 ctctttgtaa atgatctggg gtttcattta cgtaccggta aatatatata ccacagtaaa
     5401 ccaagcagca aagcgccttt ttgggagaaa gagattttcc ctgacgaaca tagcaatatt
     5461 gcgcctggta agtccgggat gtttgcatac gattaccacc agggtctgtt caggaaaaag
     5521 ttaaacggat cacgtgaatg gtcgccggta tttgagaatt ttgaactgaa agggatcatc
     5581 gacgtttttg aaacaaacgg aggcactgtt tttatcattt gcgaccgcgg ccggggcctt
     5641 ttcagatcca ctgacaatgg aaaaaactgg aaagaagtta gtcgcggcgt cataaatctg
     5701 acagcgtcaa acggggtgct gatggcgaac agcatcaatg gggtaataag atcggccgat
     5761 gatggcgaaa cctgggagac agtgatcagt gaaggcggag tgggcatcga tatagcaccc
     5821 attgatggtg gattcgctgc tatcacattc aatactcagt cggagacccg aagagtgcgc
     5881 gcttcctacg acgggggtaa aacctggcag gccatcgatg ccggactgcc accgagtaag
     5941 aatatttcct ccattatcca ggtgggtgat tctttctttt gtggtcatcc caacggcatt
     6001 taccaatcag gagataaggg gaaaacatgg aaactgatac tcccttccat agaaggaaag
     6061 gttttcagtt tatctgtttc cggcaacgtg atttatgcca tacccagtaa tggaggatgt
     6121 tgaagcatcg gtaatttgat taccctggca ctagtttgaa aaccacacat cgttttttat
     6181 cacagcggct gcatcatacc cgccagtaac acaaatctta ttattaaaaa taactgcctg
     6241 gtgacctccc ctggtaccaa aggctgcatt ggtttctttt gtccagtctt tgccgtttgt
     6301 gctgctccat atttcatcgg tagtaacaaa tccaacaagc ccacccatta catacaatgc
     6361 attattatat acgattgcct ggtgagaata gagcggtaca aattttgaac cggaggctat
     6421 ttcttctacc cagtttcttc cgtcggtgct cgaccaaaca tcgtttttaa atccggtggc
     6481 ggtatatcca ccaatcacat acaacttgct gttgaataca acaacctgat gcccggctct
     6541 cggtccaaac tgcgttcctg ttactgtttc ctgtgtccat gtaatgccat ccgtactcga
     6601 ccagatatca ttttttgtac cgccaatacc atcgccgcct attaaccaca tcttgttatt
     6661 aaatacaata acatcatgta acgaccggat actgaaagct gcattcggag ttacctgtgt
     6721 ccatgccgcg ccgtctgaac tgttccaaac gtcatttaat gcactgccat tacttaatcc
     6781 gcctattacc cagagtttat tattaaaaac aacgacctgg tgattgcttc ttacactaaa
     6841 gacagggccg cttaccgtag tttgtgccca ggctgcccca tcggtactgc tccatatctc
     6901 attcgtgtac aatatatctg atttccttcc gccaattacc caaagcttgt tgttaaacac
     6961 taccgtttgg tgatcgaaga gtggcgcaaa gtggctgccg gcagttgttg ttttcgtaaa
     7021 tctcagtgcg gcaacgggtg caggagtttt atcctttttg caggaggcca ctaaaagcgt
     7081 tgccaataat acagcaagga tactgacagg tggttttttc attttgtttg atttaaaggg
     7141 ttagattaat tttacatttg tgtaaggcat tcccccgagg ttttgcgttt ataccgcttt
     7201 ttaaattcat caggaaatgc ttctgtgctg aaagacataa agctaactac ggcatctgca
     7261 gtatcatagc atttttgata tgtgagatta acggattgat aactgaaaat taacgcagga
     7321 gccatgccca taggcaggca gacacggagg ggatgctgta caaggtctta ccacgaaagg
     7381 ctttgtagaa caacagccta atggcagcta aaaccaatag aaaagattga tttaatcaaa
     7441 attttatcat tacggaaaag ttacaattca aagttagtac ccttatttgt acctactgtt
     7501 tataacaccg gctacttaat cagcaccagt atgaaatata ttctgccttt aatatccctg
     7561 atttctcttg tcattagttg taataaaaca atacaactcc ccctcctcct tctcttccaa
     7621 cagtaataac caccgccata tcttcaataa cccctttaac cgcaacacga ggtggtgatg
     7681 tcacaaataa tggaggcgcc actgttacaa cacgtggtgt ttgctggagt gattcgcccg
     7741 ctccattgat cgcgggcaat catacaacgg atggaacagg aaccagtact ttcagcagtt
     7801 tactgaatgg cttaaatccg ggaaccacct actatgtcag ggcttatgca accaatagtg
     7861 caggaacggg ctatgggaat gaagtaagtt ttaccaccac aacccctgct gtttatacag
     7921 cgggatatga gtataataca aattcagtct gtgttgcaaa ggtctggaaa aacggagtgg
     7981 aaacagcatt atcctccggc ttctccaacg cccagggatg ggtccgcgca gtatttgttt
     8041 caggtgctga tgtttatgta gcgggttatg aatttaacgg gataaaaaat tcagcaaaac
     8101 tctggaaaaa cggaacaaca acggctttgt cggatctgac taatgattca caagcctggg
     8161 cgatcagtgt ctcaggcgcg gatgtttatg ccgcaggata tgaaaaaaac attgccggaa
     8221 tcagtgttgc aaagatctgg aagaatggtg ctcccctgtc gattactgac ggcacaaatg
     8281 atgcacaggt ttatggaatt catatagcgc aagccgatgt ttatgccgcc ggatttgaaa
     8341 acacttcagg agtaagtaca gcaaaactct ggaaaaacgg agcggttgtt ccacttcagg
     8401 gtgccggcac taacactaca acttactcgg tatatgtttc cggaacgaat gtctatgtag
     8461 ccggaagtga aattattcca ggtggatact ccagggctat cttatggaca aatggagtgc
     8521 ctgcctatct gaccagcgga atagggagta tagccattcc ctatagtgta tacgcttatg
     8581 gaacagatgt ttatgtggcc ggatttgacc agtctaccgg aaccggtctt tcaaaagcaa
     8641 cgctctggaa aaacggaatt gccagttctt taaccaatgg gtctgcttct gccagggcca
     8701 attcagttta tgtttataat aatgacgttt atgttacagg atatgaatta gtctggccat
     8761 tttcgatttc aagaacctgg aaaaacggga cggttattca ttccacgaat ggctcgttta
     8821 atgcagcaac aacttctgtg tttgttcaat agtgtggtac tgaggttaat cagcaactat
     8881 tcccatttaa tatatctgcc ggaaggaaca gatccgaata tcaacgccct aaaacgccgt
     8941 tcctgaactt tttaatttcc ccggaaataa cgaactttaa tcctgtcatc ttctgccgga
     9001 agccgcttcc ggattctggc ttgccggcgc caacctttcc aagtgtattg cttcaccttc
     9061 ttaaaataaa aggagggaat gacaatgaaa gcaatttctg ccacattact catgctggtt
     9121 ttagcctttt tatctctgtt tgcacaggct caaaaagggt catctattgt gatccacgat
     9181 aatacttctc cggccaatgg cggggaaaat gctacatcgg cactccggaa ggagatcgct
     9241 tccgcattca accgcgaaaa accctgtgtg gatctgatgg acgatgagga tataagaaat
     9301 atcatagaga gtgagcggga aaaggattta ctggaagggg ctgatccgca gcaggtatta
     9361 accgatatcg ccaacctgat gggcgccagt tatgccatga gcgtgtcgtc cacacccgga
     9421 ccagggggaa gtacctctta cacggttatc gtgatggatc cgcaaacagc caaaaccata
     9481 gcccggcaaa cgggatcaga tcctaagcaa atggctgata atatcgtgaa gcaactgggc
     9541 tccagcctgc ccgatgactg ccaaccgcat tgggtagggg aagtcaaata tgagtattct
     9601 tccaatgaaa caaaaacaac gaacgatgcc ggtgcgatgc atgccagcac gagaaatacc
     9661 aaacgtagca gcaccgttac ctactcggcc caaaacaaca tcgttgccac cctgttacct
     9721 cctaaacccg gcagtcctgt ttcctccggt aaaacaatgg ccagggtctg gatgcggtcg
     9781 aaaatcatcg gtgaactgaa acaggaaaca gcgggagaag tttattgccg gctgaaaggt
     9841 caaaatcctg tctggaaggg atacaactta aattattccg aaaccaccac acaattaggt
     9901 gggggtgcag ataatttgcc tgtttctatt tcaattgaca gtgagggcaa ctacaaaatt
     9961 tttgtaaaaa cacccggcgg aaccctttta ggaaaatttg agacgaaccg gtcggaatca
    10021 ggctgtacca ccgacaatcc ccccgtaaaa agtgcagtca gtatgcctga acaaaaactg
    10081 gatgcttcgg gttttgatgt tacaggaaaa accgatgcca aaaaccggga ttcgttatcg
    10141 ggctcgaaga ccttgcctga cggtaaaaca aaaattacct ggaacctgaa actcgtgaaa
    10201 ccgaaatcga aaaaataaac gggtcagcgt gtttattgca acctagctgt ttgtttccct
    10261 ttgtcctatt gcctgacaga aaccggtcat tgcataaagc cctgccaatg aatgcatttc
    10321 atcataatca aaaatcatct cccgaaaatg ctatatttat agccaactga atgactatgg
    10381 tgaatttcca ggaacgtgtt ttacattacc cccagtattg taaacagttt agctgtggcg
    10441 attccctgat aaccatattt aattgtccgc cggaagcccg gctgatgaaa gagaagtttg
    10501 cagacctgtg gagtcacgaa aattatatct tttttgtcct ggaagggaaa aaagtctggc
    10561 acaccgccga tggttcttac gaaataagcg aaggcagttg tgtactggtg cgaaaagggg
    10621 cctggatact ggagcagttt tttgatatcg gcttttgcct cgtgcttttt tttatacccg
    10681 atcagtttat ttgcgaaaca ctgaagagcc ggtcggcacc aatccatcat accggcaaaa
    10741 attataattc tgttattccc attgtggcaa ccgaaacact gaaatctttt tttgtttcga
    10801 tatatgccta ttttgccggc accacaagcc ccgacccctc cttactggaa ttaaagttca
    10861 gggaactcat attaaacata gcagataacc gggataatgc agaactgctt tcgtatttct
    10921 gttccataat gaatgagcct caggctgttt ccctggaaag agtgatgcat gacaattatt
    10981 gttttaattt gaaaatggaa caatatgcgg ccttgtgcaa cagaagtctt tccgccttta
    11041 aacgggattt tcaaaaacaa tttaacagca cacccggcaa atggctgctt gaaaaacggt
    11101 tacagcattc aatgacttta ttaaaaaaca cgggaaagac cgtggcggag gccgcctttg
    11161 aaagcggatt cgaaaacaat tctcacttca gccggtgttt taaacagcgc ttcggcctca
    11221 ctcccatggt cgccaaacaa tcaatggttt cctgagcctg tcagcaaaac aggtggactt
    11281 cagagcaaag acaggtttat gtacaggccc tattttcgtc ctgtaataaa aaagttattt
    11341 tctcttacct ctaaacaaaa accaatgaat ccaaacaaag cactttggga aaaaggtgat
    11401 ttcaccaaga tcgcagacac catgcgggaa agctccgcag cactagttgc aacactgggt
    11461 atccaacctg gtatagctgt tttagacgtc ggatgtggtg atggcgacac ggccgtaccc
    11521 gccgcaaaac tcggggcaca ggttacgggc cttgatattg cccacaatct ggtggaagcc
    11581 ggtaatctgc gggccaataa agaaggcgtg gccgcttcct gtaaaattct ggaaggagat
    11641 gtaatggata tgaaggagct gaaagatgcc aattacgatc ttgtcatcag tatttttggc
    11701 gccatgtttg cgccccgccc gctagatacc gctaaagaaa tggtgcgtgt tacccgtaaa
    11761 ggcggccgta ttgtaatggg caactggata cccggtgatc caaccctggt ggcacagatc
    11821 ctgaagatca gttccgcgta tacgccggca ccccccgaag gttttatcag cccgatgcgc
    11881 tggggcgtgg aagaagacgt aatcagtcgt ttcggtaacg caggtattgc aaaagaaaac
    11941 atctccttct ccagggaaac gtttacgttc caggcagatt atgccccgtc tgtactggtc
    12001 aataatttca aaaattatta tggccccacc atgaatgcct ttgaagccgc tgaaaaaacc
    12061 ggtaatgcag gagcgttgca actggagctg gaaaatttgt ttaacagcca gaatcaaagc
    12121 gggatcggaa accgaacctc cattccggct acttatttaa aagtaactgt acgctgcccg
    12181 taatcggtat cagggcctcg gtacccggcc cctatttctt aaaaaacgga acttccgggc
    12241 agcctggact ttgcctgcgg gtcgccggac tatagtatat tgcataataa acagtatgaa
    12301 aaaatacctg ctgttctgcc tgctggtact ttccctgaaa agttatagtc aatgcaccga
    12361 atgtaactcg gtgcatttgg cattgaaaaa acccgggcag gtcagatcgc ttatcctgaa
    12421 cgggcatcag catggcgtgg taatggacag tatcccggta gcgattggtc aactggtgaa
    12481 ctgtgaaatc ctgtatctca ctgatcaccg gataaaaaaa gtaccggcca ctatcgggaa
    12541 actgcagaaa ctgaaggaac tgagttttgg aggttgccaa ctggagcggc tgccggagga
    12601 aatcttcagt ttaaaaaacc tgaaagagct gattcttttt gacaatgcat tttctgaaga
    12661 atatataaag gaattaaggc aacgggttgc cacggcttta ccgaaaacga agctgatgtt
    12721 gtaagaggtt atagtttaat taagaataat ccggttttat tttatgcccg aatacgaaaa
    12781 attccaggcc tgctacgaac aggaaggtga caaactcttc ctgcagttca accagtttac
    12841 agaagagcag ctgctttccg agaactggaa aaacgccttg tcctgaagtc ccattgaaaa
    12901 gggatctata tctttacttc ccaaaaagca aaaaccccga tgcgcataat cctccccctc
    12961 ctttcccttt ttatcaacct ggtgaacagc catgcacaaa acagtgcgcc taaactaaag
    13021 atcactcccc tcaccggcga tttctatatc tataccacgt ataatacata tgaagacagt
    13081 caggtacctg ccaacgggat gtaccttgtt acggacaatg gagtagtcct ttttgacaca
    13141 ccctgggata ctacccaatt ccaacccctg ctggacagta tcaagctaaa acataataaa
    13201 aaagtaaacc tctgtattgc cactcactgg cacagtgaca agacggccgg actggaatat
    13261 taccggcagc agggtatccg gacctatacc acaaaactga cggatgaatg gtcacataaa
    13321 aacaatatgc ccagggctga attcctgatg tacagtgata ccgtgttcca ggcaggtcag
    13381 tatcggtttg aaacatacta cccgggtgaa ggacatacgg ctgataatat tgtaatctgg
    13441 tttaacaagg aaaagattct ttatggtggc tgtctcatca agggggtaag cgatgtgagc
    13501 ctgggctatt taggagatgc caacagtata gcctatgcca gcacgctgaa aaatgtacgg
    13561 aaaagatgcc ggaaaccaag atcgattatt atcgcacaca atgactggaa caatctgaat
    13621 tcattgaaac actccataaa gatggctaaa agactaaaaa gaaaacagta gtacctccat
    13681 tgcctccttc tgcttcttgt aactgacctg atacacttct cctgtttatt cctgactagt
    13741 tacctttcag cagccattca aagccggcgt tctgcaggaa acttcatgcc cactataaaa
    13801 cctctcccgc aaatgtgtga atagtctaac aattcagtaa tttgtataac agttgcccat
    13861 tcccggctgt ccatctttgt acgctggcaa taatcaccat attgattgaa ctaaatccag
    13921 gataactttt gaaagaactc gaagattata gctggttccc ctcagtatta agaaaatttc
    13981 aaacggaatt cattggcttt gtcgtagccc ggttcaatgt atatgatggg tttgtaaagc
    14041 acctgaacac attatcattg cccgcgcagc ccctgaccga tctttgctcc ggcagcgggg
    14101 agcccgccat ccacatcttc aaaaaaagcc attgtttcac ccatcttttt ctgagtgaca
    14161 aatatcctgc gagccaggta gttaaatcgg gtgaggattc ctatgcgatg gcgcggaaag
    14221 atgtccttga aatggaattc aaccccggga cctgctattc catgttcaac gcgtttcatc
    14281 atttcagtga tgaagatcaa ttaaggatta tacggaaact gcatcactcc gggtccacgg
    14341 cttttctggc ggagatcctg gagccggata ttatctgttt cctgaaagta ctctttacca
    14401 ccactattgg ctgcctgcta ttgaccccgt ttataagacc cttttcgttc aggcggcttt
    14461 ttttcactta tctgttgcct gtcaatatcc ttaccatcac ctatgatggt ctcgtatcgg
    14521 tactgaaatc caggaccgca aaccactacc ggaaactatt cgcggaccag ggagaaggta
    14581 ttgaagtcat ccgtttaaga aacggcctga ctcctgttat catcatacag attcatgcgg
    14641 ggacatgaaa acgctcctga cgatctggaa attcagccgc ccccatacca ttattggaag
    14701 cgtgatcagc atcatcactt tgtattttat cgtgtgtgac aaaaaggagg tccagtatct
    14761 ttccttcctg gtaccggccc tgatcatcgg gatcacttgt aatattttca ttgtaggcat
    14821 taatcagata gccgatatcc aattggataa gatcaacaaa cccaacctgc cactgccgtc
    14881 gggtgcgcta agcccggcac aagcctggct gattgtaagc attgccttat cgatcagtct
    14941 tggattggcc ttattcatta ct
//
LOCUS       DAOPMW010000041        14586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4158_length_14586_cov_8.167160, whole genome shotgun sequence.
ACCESSION   DAOPMW010000041 DAOPMW010000000
VERSION     DAOPMW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_14586_cov_8.167160"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..470
                     /gene="trxA"
                     /locus_tag="PLU11_03740"
     CDS             153..470
                     /gene="trxA"
                     /locus_tag="PLU11_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPN58255.1"
                     /translation="MAKEFTDANFKNDVLASDKLTVIDFWAEWCGPCRAIGPVIEELS
                     KEYDGKANIGKLNVDNNPEVSMNYGITSIPAILFIKNGQVVDKLVGAQPKANFVRKIE
                     SHI"
     gene            complement(541..1797)
                     /locus_tag="PLU11_03745"
     CDS             complement(541..1797)
                     /locus_tag="PLU11_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPN58256.1"
                     /translation="MNIKGRLTVLSFMQFFVWGAWLLTIGTYCFNAKGWTGAQFGAIF
                     STLALSSLFMPALTGIIADKWMNAEKLYGILQILYGVVLFFVPQVNDPDTLYYVIFAA
                     MICYMPTISLSNSISYTILKNNNYDVVKVFPPIRVWGTIGFIVALWTTNLTGSKANAN
                     QFIIAAVAAIALGIYAFTLPKCPPQRSISKDASLIEQLGLNAFKLFSKYKMALFFIFS
                     MFLGAALQLTNMYGDSFLDDFRKIPEYGPESFVVKYSTIIMSISQISETVFILTIPFF
                     LKKFGIKKVMLFSMIAWVLRFGLFAYGNPTDGLWMIILSCIVYGMAFDFFNISGSLFV
                     ETTTSSSIRSSAQGLFMMMTNGVGAYMGSKISGYVIDQYFTIGAGDKDWHNIWLYFAL
                     YALVVGVLFALLFKHKHNPDELNPQH"
     gene            1916..3058
                     /gene="mqnE"
                     /locus_tag="PLU11_03750"
     CDS             1916..3058
                     /gene="mqnE"
                     /locus_tag="PLU11_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HPN58257.1"
                     /translation="MVTDIVLAPELQSIAAKVKAGERISDADCLTLFEKASLGFVGSL
                     ANSIKERLHGDKVYFNRNFHIEPTNVCVFSCNFCSYSRLYANKEEGWELTIQQMLDMV
                     KKYDGKPVTEVHIVGGVHPKMDLNFFADLLKQIKAHRPELHIKAFTAVEYDYMFRKAK
                     LSVEEGLKFLIEAGLDSIPGGGAEIFHPEIRQKICADKVDADGWLKIHETAHQLGLHS
                     NATMLYGHIEHYTHRVDHMRRLRELQDKTKGFNTFIPLKFRNMDNDMSNIAESTHIED
                     MRLYAIARIYLDNFPHLKAYWPMLGRQQAQLSLSFGVDDIDGTIDDTTKIYSMAGSEE
                     LNPSMSTADLVSLIRQAKREPVERDTLYNEIQNFGKTGAINSPELN"
     gene            complement(3129..3761)
                     /locus_tag="PLU11_03755"
     CDS             complement(3129..3761)
                     /locus_tag="PLU11_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58258.1"
                     /translation="MATKKQAKKKVAKPAARKKTAPKKKAVKKVAKKAVKKPVKKAAK
                     KVVKKAAPKKAAKKKAVKKVAKKAVKKVAKKVVKKAVKKAAPKKVAKKAAPKKAAPKK
                     PVAKKPVTKKPVVKKTVAVKPAPPKAVVKKPVAKKPVVKKTPAPKPAPAATVVPVMVE
                     EITEVFSTPEGEVEVTQTVLTFDEEAGEEGNGFTTDDVTDDEENKEDASA"
     gene            complement(3892..5319)
                     /locus_tag="PLU11_03760"
     CDS             complement(3892..5319)
                     /locus_tag="PLU11_03760"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPN58259.1"
                     /translation="MPYEAISVFDIFKIGVGPSSSHTLGPWQAARRFVQSLQDNGQLP
                     KVTQLRVLLYGSLAKTGKGHGTDIAVQLGLGGEDPVNFAVDQIDARIRDIASMHKLIL
                     NGNHEVEFNPAEDIEFLFAESLPFHPNAMTFLVQLNDDTAVAETYYSVGGGFVIKEGE
                     VRAAGSSVQLPFPINTADDLLHWSMKTGMSISDIVLENENTWRKEEETRHSALNIWRV
                     MKECMYRGCHTSGILPGGLNVKRRGAALNQKLLAGKNYSDYDSWLTAIRTGGNDFKYT
                     LDWVSCFALAVNEENASFGRVVTAPTNGAAGVIPAVLHYYIAFCDGNREEKVVQFLLA
                     AGEIGSIFKKGATLSAAMGGCQAEIGVSSAMAAAGLTECMGGSQKQVMMAAEIAMEHH
                     LGLTCDPIGGLVQVPCIERNTMGAIKAITASQLALQSTPDFAKVSLDAVIKTMWETAL
                     DMNHKYKETADGGLAINVPLSLPEC"
     gene            complement(5414..5881)
                     /locus_tag="PLU11_03765"
     CDS             complement(5414..5881)
                     /locus_tag="PLU11_03765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPN58260.1"
                     /translation="MKNFIVSVFVSLLILPGTGCLKDNSCNAKSVDSERTTMSTYATA
                     NGLTVTEHSSGFQYQIISAGAGQTPSLSSTVSVRYTGKLMDGTVFDSATGTPVTFPLG
                     QVIPGWQLAVPLIQEGGLIRIILPSSLAYGCTGAGAIPPDAVLYFEIQLVDVQ"
     gene            complement(5953..6270)
                     /locus_tag="PLU11_03770"
     CDS             complement(5953..6270)
                     /locus_tag="PLU11_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58261.1"
                     /translation="MLSVEEKAFVTYWGENRLSKKRVLKQLYAGLPLAMILVVAIFAN
                     LFSGWYGRAQMVLFRENSSLIIILLVAALSIVVFIVIFSARHRWEQNELRYRELLHKE
                     EKD"
     gene            6360..7304
                     /locus_tag="PLU11_03775"
     CDS             6360..7304
                     /locus_tag="PLU11_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58262.1"
                     /translation="MKLALLLAQYLYANKKLDLPGIGTFHLDPSVVIDPETDKNTKPE
                     PINGIHFDYNTSIKENTELTDYIAAKSGKIKALAAADLDSHLWLGQQFLNIGKPFVLE
                     GIGQLTKTQAGALDFSAGILQPEVLKDQQNWQPMADHSSQAGENDYKDLLNNRRDTVQ
                     WKKPVAIIMIIAGIGVAIWGGYTVYKNNSAEAGADSLAKESTTTGIQPQVNSGITGNT
                     SSPSDTSHSTSVNEISAPATPAGMYKFVVETAGRQRALTRYQKLKALPTDVKMETPDS
                     LNFKLYFLLQVSPADTAKVLDSLRTYYTPKGNRAFIEK"
     gene            complement(7358..8353)
                     /locus_tag="PLU11_03780"
     CDS             complement(7358..8353)
                     /locus_tag="PLU11_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPN58263.1"
                     /translation="MAKQTISNIKLGVFVITGLLFLILLLYMIGKNENLFGSTFRLRA
                     QFGNVQGLTSGNNIRYAGIQVGTVKKVRILNDTLIEVVMLIETKMKDFIRKNAVVSIG
                     TDGLMGNHVLNITPVAGTADLVGDNDILPVKKMESVDDMMQTLAKTNEDVAVIASQLK
                     NTVLRINSSTAVWEILDDKTVPQHIRSSLINVRSATAKASRLISDLQLVLSDIRDGKG
                     SLGAIITDSSFAQNLQAALLRINEVGADADTLAKTINSLTRQVQDDLDRGGGTANVLL
                     KDSTFARNLRESILNIREGTDGFNEIMEAMKHSFLFRGYFRNLEKQKKANLLLKQ"
     gene            complement(8360..9139)
                     /locus_tag="PLU11_03785"
     CDS             complement(8360..9139)
                     /locus_tag="PLU11_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN58264.1"
                     /translation="METAFIIPVKPVATGTKKEEVIRIQGLYKSFGTNQVLIDFNLQL
                     FAGENVVVLGKSGSGKSVLIKCIIGLLQPDQGLVQVFGKNIPDLSHEELDKIRMKVGF
                     LFQSNALYDSMTVKENLEFPLRRHSIRMEQQEVDELVLNALDDVGLAHTINLMPAELS
                     GGMRKRIALARTLILRPDIILYDEPTTGLDPITGKEIIELMVDIQKKYKSSSLIISHD
                     MNCVRMAADRVVMLIDGKCYVQGSYDELKRSADPRVKEYFE"
     gene            complement(9142..9930)
                     /locus_tag="PLU11_03790"
     CDS             complement(9142..9930)
                     /locus_tag="PLU11_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN58265.1"
                     /translation="MSKRTEIMQIKNWKNWFDEAGDMSRFAGRFFSQWFRPRYEVAEF
                     FRQCFIIGYKSLPLVGLTGFIMGLVLTMQLRPSLIDYGVESQLPAMVGIAIVREIGPV
                     IAALIFAGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKYLVVTRVLATTLMLPVLVM
                     LGDAISLYGSYLGVNIRGVTSFQLFWTQVFDNLSFSDVLPAFIKSFFFGFAVGVVGCY
                     KGYNSRKGTEGVGRSANSAVVLSSILIFIIDLIAVQVTDIFELN"
     gene            complement(9923..10258)
                     /locus_tag="PLU11_03795"
     CDS             complement(9923..10258)
                     /locus_tag="PLU11_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPN58266.1"
                     /translation="MNATTSEPVRAVDYKRLRVECTAFPEGGLIPERYTCDGKNISPP
                     FIIHHIPEEAVCLALIKEDPDAPGGTWVHWLLWNMPVTSHIVAFGEYSGLYKRNEYGE
                     EGPVAYEDE"
     gene            complement(10285..10476)
                     /locus_tag="PLU11_03800"
     CDS             complement(10285..10476)
                     /locus_tag="PLU11_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58267.1"
                     /translation="MAKNLKPLISPEIPYPGKRPDTIPDTGDEELQLPEDDPDIIIDP
                     ETEEETEPAFIPPPPGEGP"
     gene            complement(10499..11431)
                     /locus_tag="PLU11_03805"
     CDS             complement(10499..11431)
                     /locus_tag="PLU11_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HPN58268.1"
                     /translation="MRRYFTQSARFNETEAVNLLKQRSCSLNTKKDLEPLFNKIGDAK
                     IVMLGEASHGTHEYYTWRAYITRKLVEEKGFQLIAVEGDWPDCYRLNRFVKGLDKTSK
                     SAFDVLHVFNRWPTWMWANWEMVALAEWLHRFNSELPANKRVGFYGLDVYSLWESMEN
                     IMQYLSKTDPEALKIAEEAFRCFEPFRKGEGSDYARAAQFVPELCQQEVVSLLKTIQE
                     KSAGYNTDTENVFSAGQNARIAVNAEKYYRAMVTGGPHSWNVRDRHMADTLDALLDFH
                     GRNAKVVVWEHNTHIGDARATDSTQIPETYPFGV"
     gene            11591..12115
                     /locus_tag="PLU11_03810"
     CDS             11591..12115
                     /locus_tag="PLU11_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HPN58269.1"
                     /translation="MTTNDTAISNLHQLLKSDICRQASAEVQLHKVLPGWASLATSLK
                     LKVVIQKYLDFVVLQKQEIDQFIEMENINQLPLCNRVMQAFILDTEEKAGYCSDSEVK
                     DACLLASIQEINHYKISAYGTAAAFANTLGMEATALVFRKAEVNEKQIDDRLSQLAEH
                     EINLKARTPIVLPG"
     gene            12126..12779
                     /locus_tag="PLU11_03815"
     CDS             12126..12779
                     /locus_tag="PLU11_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN58270.1"
                     /translation="MDWRLNDTVIIPVQNSFVYGELEIPLHAQSVVIFSHGSGSSRLS
                     PRNQFVARYLHQQNIGTLLFDLLTEKEDLNYQNRFDITLLTKRLIRATEWLVEQPVAR
                     QLSLGYFGASTGAASAIRAASLLPNIQAVVSRGGRPDLAGTVYLIKLEAPSLLIVGSL
                     DTEVIRLNEWAAAQIKGKNKLEIVPGATHLFEEAGTMDKVCELAAAWFRHYLIPVEA"
     gene            12822..13454
                     /locus_tag="PLU11_03820"
     CDS             12822..13454
                     /locus_tag="PLU11_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPN58271.1"
                     /translation="MFRNRTDAGTRLAEKLKKFKKEPGIIMAVPRGGVPVAYAVAKEL
                     NLPVELVLTKKIGHPLNKEYAIGAASLTDYFIIPHEGVTDHYIESELQLIRSRLKEMQ
                     KTLQADTLPENYEGKTLLIIDDGIATGNTILGTIKLLHKNRPGKIVIAAPVASEPAVE
                     MLAKEADEVIVLLIPSEFYGVGAFYEDFRQVSDEEVMYYLNKLKELRKAG"
     gene            complement(13629..>14586)
                     /locus_tag="PLU11_03825"
     CDS             complement(13629..>14586)
                     /locus_tag="PLU11_03825"
                     /inference="COORDINATES: protein motif:HMM:NF023425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HPN58272.1"
                     /translation="SEQTFPTHAAILGNGETLLPGTYLIAEASSIEGNLVMDAGGNAN
                     AIFIIKIEGAFSPGPSSQVILTGGALACNIYWAVEGGAIAMAASSKMKGNFIANPGAV
                     SMAASSSLEGRLLSTTGAISVDGVTAAIPVCSALLPLILTDFTLQKQGNAVVLLWTGK
                     DEHSFAGYELQRSQDGFIFNSIGFVVSSGDTGPVNYQWTDQQPLPAMSFYRLKMIDRD
                     GKFTLSHVLFVSQNPLTGFSIFPNPVSAGQVQLQMASVPKGNYQMRIYNEAGIRMKEL
                     DIKIGNETVNIITVPLRLSAGFYFLVIHDPGGSRQAMKFIMK"
BASE COUNT         4047 a         3510 c         3143 g         3886 t
ORIGIN      
        1 tagtacgatc ttttaacagc tgttaacaag tttccctgac aagttttcaa ccgtggagcc
       61 aagttttaca caactttgtg ccagtatcac atgttatcag ggcaggactt aaatttgcta
      121 tccttaaaag aacaaaacaa aaaaactata caatggcaaa agaattcacc gacgcaaact
      181 ttaaaaatga cgtattagca tctgataaac tgactgtgat cgatttctgg gcagaatggt
      241 gcggtccctg ccgcgctatc gggccggtta ttgaagaact gtctaaagaa tatgatggta
      301 aagcaaatat tggtaaactg aacgtggaca ataaccctga agtttccatg aattacggga
      361 tcacttctat cccggctatt ctctttatta aaaacggtca ggtagtcgac aaattagtag
      421 gcgctcagcc gaaagcaaat tttgtaagaa agattgaatc acatatttaa ttttctataa
      481 acaaatatta aaaagcccgg acttccgctg aagcccgggc tttttagtat acagagatgg
      541 ctaatgttgc ggatttagtt catccggatt gtgtttgtgc ttgaatagca gggcaaaaag
      601 gacgcctact acaagggcat aaagagcaaa ataaagccag atattatgcc agtctttatc
      661 acctgctcca atggtgaaat actgatcgat tacatagccg ctaatcttac ttcccatata
      721 ggcgccaact ccattggtca tcatcataaa gagcccctgg gcacttgagc gtatagatga
      781 actggtggtg gtttcaacaa aaagggaacc ggagatattg aaaaaatcaa aggccattcc
      841 gtatacaata cacgacagaa tgatcatcca taatccgtcg gtcggattcc cgtaagcaaa
      901 gagtccaaaa cgaagcaccc aggcaatcat ggagaacagc attactttct ttataccgaa
      961 ttttttcagg aagaacggga tcgtcagaat aaaaacggtt tccgaaatct gggaaataga
     1021 catgataatc gtggaatatt tcactacaaa agattcaggt ccgtattccg gtattttcct
     1081 gaaatcatca agaaaggaat ccccatacat attcgtcagc tgcaaggcgg cacccaaaaa
     1141 catggaaaaa ataaagaaca aggccatttt atatttcgaa aacagtttaa aagcgtttaa
     1201 acccagctgt tcaatcaggc tggcatcttt tgaaatggat cgctggggcg ggcattttgg
     1261 caatgtaaac gcataaatac ccagggcgat agccgctaca gccgcaatga taaactggtt
     1321 ggcattggcc ttactgcccg tcaggttggt ggtccacaag gcaacaataa atccgattgt
     1381 tccccacacc cgaatgggcg ggaatacctt taccacatca tagttgttat tcttcagaat
     1441 ggtataagaa atggaattgg acagggaaat ggtaggcata taacagatca tggccgcgaa
     1501 aatcacataa tacagggtat ccgggtcatt cacctggggg acaaaaaaga gtactacacc
     1561 atagagaatc tgcaggatac catacaattt ttccgcattc atccatttat ctgctatgat
     1621 cccggtaagg gcaggcataa acaaagagga tagtgcgagg gttgaaaaga tggcgccaaa
     1681 ttgggcaccg gtccagcctt ttgcattaaa acaataggtg ccgattgtca gtaaccaggc
     1741 accccataca aaaaactgca tgaaactaag taccgttaac ctgcctttaa tattcatacg
     1801 gaaaatttaa aatgaacact ttaaaaaaga aagatatgaa tttttccggc ccggaaattt
     1861 ttcaggaatt cagagccgga acctgcttaa ctttgcctat taatctgttt aaatcatggt
     1921 gaccgatatt gttttagcgc ccgaactgca atccatcgca gccaaggtaa aagccgggga
     1981 acgtatttcc gacgcggact gtctgacctt gtttgaaaaa gccagcctgg gttttgtagg
     2041 gagcctggcc aattccataa aagaaaggtt acatggcgac aaagtctatt tcaaccgcaa
     2101 tttccatatt gagcccacca atgtctgtgt attcagctgc aatttctgtt cgtacagccg
     2161 gctttatgcc aataaggaag aaggctggga actgaccata cagcagatgc tggatatggt
     2221 aaaaaaatac gatggcaagc cggtcacgga agtgcatatc gtgggcgggg tacacccgaa
     2281 gatggacctg aatttctttg ccgatctgct gaaacagatc aaggcccacc gccccgaatt
     2341 acatatcaag gcctttaccg ccgtggaata tgattatatg ttccggaaag ccaaactctc
     2401 cgtggaagaa ggtttgaagt tcctgattga agccggcctg gactccattc cgggtggagg
     2461 cgcagaaatt tttcatcccg agatccggca aaaaatctgt gcggataaag tggatgcgga
     2521 tggctggctg aaaatacatg agacggcgca tcagctcggg ctgcattcca atgcgacgat
     2581 gctgtatggt catattgagc attataccca tcgggtggat catatgcgtc ggttacggga
     2641 gttgcaggat aagacgaagg gctttaatac gtttattccg ctgaagttcc ggaatatgga
     2701 caatgacatg agtaatatag ctgagagtac gcatatagaa gatatgcgac tctacgcaat
     2761 tgcccggatc tacctggata atttccctca tctcaaagcc tactggccca tgcttgggcg
     2821 tcagcaggcc cagttatccc tttcctttgg cgtggatgat attgacggta ccattgatga
     2881 taccacgaag atatacagta tggccggctc tgaggaactg aatccatcga tgtcaacggc
     2941 ggacctggtc tccctgatcc gccaggcgaa aagagaaccc gtggagagag ataccctgta
     3001 taatgaaata caaaatttcg ggaaaacagg agctatcaat tcccctgaac taaattaatc
     3061 tcctgtttat aaaagaaaga ggttatcacg gaatgtgata acctcttttt atttgttggc
     3121 cagacagctt aagctgaagc gtcttcctta ttttcttcgt catccgtaac atcgtcagtc
     3181 gtgaacccat ttccctcttc gcctgcttcc tcatcgaaag tcaggacggt ctgggtaact
     3241 tccacttcac cttccggagt actaaacact tcggtgattt cttccaccat tacaggaacg
     3301 actgtagctg caggagcggg tttgggtgcc ggtgtttttt taaccactgg ttttttggcc
     3361 accggttttt ttaccacggc tttaggcggg gcgggtttta cagctactgt ttttttaact
     3421 accggctttt tagttactgg ttttttagct actggttttt tgggagctgc ctttttgggt
     3481 gctgcttttt ttgcgacttt tttaggcgct gcttttttta ccgctttctt caccactttc
     3541 ttcgcgactt tttttaccgc ttttttggct acttttttaa cggctttttt ctttgctgct
     3601 tttttgggcg ctgctttttt caccactttt ttagcggctt ttttaacggg tttcttaacc
     3661 gctttttttg ccactttttt tactgctttc ttttttggag cagttttttt acgggcagcc
     3721 ggtttggcga cttttttctt cgcttgcttt ttagtagcca tgacagataa tttagaattg
     3781 gataaaatca ggttttgaag tgaaataaat ttacaacatc ctttttgtta tgtggtaact
     3841 tattccaaaa ttaatataaa caaccgggaa ttacataacc tttcccatgg ctcagcactc
     3901 cggtaagcta agcggaacat taatggccag tcccccatcg gcggtttcct tgtatttgtg
     3961 gttcatgtcc agggccgttt cccacatggt tttgatcacc gcatccaggg atactttagc
     4021 aaaatccggg gtactctgca gggccagctg ggaagccgta atggctttga tggcccccat
     4081 ggtattccgt tcgatacagg gaacctgtac caatccccca atgggatcgc aggtgagtcc
     4141 gaggtggtgt tccatcgcta tttccgcggc catcatcact tgcttctggg aaccacccat
     4201 acattcggtc agtccggcag cagccatagc cgacgacacc ccgatctccg cctgacaacc
     4261 acccatggcc gcagaaaggg tcgccccttt tttaaatatg ctgccgattt ccccggccgc
     4321 cagcaggaac tgaactactt tctcttcccg gttcccgtcg caaaaagcga tataataatg
     4381 cagcacagca ggaatcacgc cggcagctcc attcgtggga gccgtcacta cccggccaaa
     4441 ggaggcattc tcctcattca ccgccagggc aaaacaactg acccaatcaa gggtatattt
     4501 aaaatcattg ccccctgtac ggatcgccgt cagccagctg tcgtaatctg aataattttt
     4561 cccggccagc aatttctggt tcagggcagc gccccttctt tttacattta atccaccggg
     4621 taaaattcca gatgtatgac acccgcggta catgcactcc ttcatcaccc gccatatatt
     4681 cagggctgag tggcgggtct cctcctcttt tctccaggta ttttcattct ccagaacaat
     4741 atcggaaata ctcatcccgg ttttcatgga ccagtgcagc agatcgtcag cggtattgat
     4801 ggggaatggc aattgcacgg agctgcctgc agcacgtact tccccttcct taataacaaa
     4861 gccacctccc accgaataat aggtttcggc aacagcggta tcatcattca gctgtactaa
     4921 aaaggtcatg gcgttggggt gaaatggaag ggattcagca aagagaaatt cgatatcctc
     4981 cgccgggttg aactccactt cgtggttgcc gttcaatatc agtttatgca tgctggcaat
     5041 atcccggata cgggcatcga tctgatccac cgcaaaattc accgggtctt ctccgccaag
     5101 tccgagttgt actgcgatat ctgttccgtg accttttccg gtcttggcca gggagccgta
     5161 taataaaacc cggagctgtg tgactttcgg taactgtcca ttatcctgca gggattgtac
     5221 aaaacgccgg gcagcttgcc agggcccaag ggtatgggag ctggaggggc caacccctat
     5281 tttgaaaata tcgaaaactg atatggcttc gtatggcaaa atgtggtttt gatttcaaaa
     5341 atacaggaaa aaagaacccc gccactgcaa gcaggacggg gttagcagag cataagcagt
     5401 aaaggctcag caattattga acatctacaa gttgaatctc aaaatataag actgcatccg
     5461 gcggaatcgc acccgcacct gtacatccat atgccaggga ggatggaaga ataatccgga
     5521 ttaacccgcc ttcctgaatc aggggaacgg ccagctgcca gccgggtatc acctgtccaa
     5581 ggggaaaagt tactggtgtt ccggtagcgg agtcaaaaac ggttccgtcc atcagtttac
     5641 cagtatagcg tacggagact gttgagctta gtgagggggt ttgcccggct cctgcggaaa
     5701 taatctggta ctggaacccg ctgctgtgtt cggtaaccgt caacccgttg gcagtggcat
     5761 aagtcgacat ggtagtacgc tcactgtcca ctgattttgc attacaacta ttatccttca
     5821 ggcaaccggt gccgggcaaa atcagcaagc ttacaaaaac actaacgata aaattcttca
     5881 tactcaacaa ttggtgcaaa tccggaataa gacctcctta aaccctgatt cgctgcaaat
     5941 aaaataaaaa aatcagtctt tttcttcttt gtgcagaagc tcccggtacc ggagttcatt
     6001 ctgctcccat cgatggcggg cagaaaaaat gacgataaag accacgatgg agagggcggc
     6061 caccagtaaa atgatgatca gcgaggagtt ttcccggaac agtaccatct gggcccgtcc
     6121 ataccagccg gagaacaggt ttgcgaaaat ggctaccacc agtatcatgg ccagtggcag
     6181 tccggcgtac agctgtttca gcacccgttt tttggacagc ctgttttccc cccagtaggt
     6241 cacaaacgct ttttcttcaa cacttagcat aattcatgca aatttaaagt atcccgacca
     6301 acagtacttg tcacaatcct ataaaatcta tatattgtag tctgtaaaaa tccaaaacat
     6361 tgaaactagc actgctcctg gctcagtatc tgtatgcgaa taaaaagctg gaccttcccg
     6421 gcatagggac ttttcacctg gatccctctg tggttattga ccctgaaacc gacaaaaaca
     6481 ccaaaccaga acctataaac gggatccatt ttgattacaa cacatccatc aaggaaaata
     6541 ctgaattaac ggattatatc gctgctaaat ccggtaagat caaggccctg gcagctgctg
     6601 atctggattc acatctttgg ctgggccagc agtttctgaa tatcgggaag ccctttgtgc
     6661 tggaaggcat tggccagttg acgaaaaccc aggcgggtgc ccttgatttt tctgcaggca
     6721 ttcttcaacc cgaggtatta aaagaccagc aaaactggca gcccatggcc gatcacagtt
     6781 cccaggccgg ggaaaatgac tacaaagatt tattgaataa ccggagggac actgttcaat
     6841 ggaagaaacc cgtggctatt attatgatta ttgccggcat cggggtggct atctggggcg
     6901 gttatactgt ttataaaaac aatagtgcag aagctggtgc cgactccctg gcaaaggaaa
     6961 gcactaccac gggtatacaa ccccaggtga attcgggcat aacagggaac acttcctccc
     7021 cttcagatac cagccattct acctccgtta atgaaatatc cgctccggct accccggcag
     7081 gtatgtataa atttgtggtg gaaacggctg gtcgtcagcg agccctgacc cgttatcaga
     7141 aattaaaggc cctgcccacg gatgtgaaaa tggaaacacc ggactccttg aatttcaaac
     7201 tctatttttt attacaggtc agtccggcag atacggcaaa agttttagac tccctgcgga
     7261 cttattatac accgaagggg aatagggcgt ttattgaaaa ataacaaagg acaaccctta
     7321 attcaaggga tcaatacccg tgctaatgat cctgtactca ttgcttcaaa agcaagtttg
     7381 ctttcttttg tttttccaga ttccggaagt acccccgaaa aagaaaacta tgcttcatgg
     7441 cttccatgat ttcattaaac ccgtcagttc cctcccggat attcaatatg ctttcccgca
     7501 aattccttgc aaaggttgaa tctttcagta aaacattggc cgttccccca cccctgtcca
     7561 ggtcatcctg cacctggcgg gtcagactat taatcgtttt ggccagtgta tcggcgtctg
     7621 cccccacttc attgatacga agcagggcag cctgaaggtt ctgtgcaaag ctgctatccg
     7681 taataatagc tcccaacgaa cctttcccat cccgaatatc cgacagtacc agttgaagat
     7741 cgctgatcaa ccggctggct ttggcggtgg ctgaacgtac attgatcagt gaggaccgga
     7801 tatgctgtgg taccgtttta tcatcaagta tttcccaaac agctgtgctg ctattaatcc
     7861 ggagtacggt attttttaac tgggaagcga ttactgcgac atcttcattt gttttggcca
     7921 gggtctgcat catatcatcc acgctttcca ttttctttac cggcaggata tcattatccc
     7981 cgacaagatc agccgtaccg gcaaccggtg taatattcag tacatggttt cccatcaggc
     8041 catccgtacc gatagaaacg actgcatttt tacggataaa gtctttcatt tttgtttcaa
     8101 tcagcattac cacttcgatc agggtatcgt tcagaatccg cactttttta acggtaccca
     8161 cctgtatacc agcataccgt atattattgc ctgaggtcag gccctgcaca ttaccaaact
     8221 gggcccgcaa gcggaaagta gaaccaaata aattctcatt ctttccgatc atatataata
     8281 acaggatcag gaaaaggagg ccggttatca caaacacccc cagttttata tttgatatcg
     8341 tttgctttgc cataaaaatc tattcaaaat attccttcac tctcgggtcg gcggaccttt
     8401 tcagttcatc gtaacttccc tgcacgtaac atttaccatc tatcagcata accaccctgt
     8461 ctgcggccat acgcacacaa ttcatatcat gagaaataat cagcgatgat gatttatatt
     8521 ttttttgaat atcaaccatc agttcaataa tctcttttcc cgtgatggga tccaaccccg
     8581 tggttggttc atcgtacagg atgatgtcgg gtcggaggat cagcgtccgt gcgagcgcga
     8641 ttcttttacg catgcccccc gacagttcgg caggcatcag gttgattgta tgagccagtc
     8701 ccacatcatc gagtgcattc aacaccagtt catccacttc ctgttgttcc atccggatcg
     8761 aatgccggcg taacggaaac tccaggttct ccttcactgt catcgaatca tacaaggcat
     8821 tgctttgaaa aagaaagccc actttcattc ttattttatc cagttcttca tgggaaagat
     8881 caggtatatt tttcccaaat acctgcacca ggccctggtc aggctgcagc aacccgatga
     8941 tgcatttgat cagtaccgat ttacctgagc ctgattttcc caataccacc acattctcac
     9001 cagcaaacag ctgaaggtta aaatcaatca gtacctgatt cgtaccgaat gatttataca
     9061 aaccctgtat ccggatcact tcttcctttt tagtccccgt agcaaccggt ttaactggta
     9121 taataaatgc agtttccatt ttcagtttaa ttcaaatata tccgtaacct gtactgcgat
     9181 caggtcaata atgaaaatca ggatggagga cagtaccacg gccgagttag cagaacgtcc
     9241 caccccttct gtccctttcc tggaattata ccctttataa caacccacca cacccacggc
     9301 aaacccaaag aaaaaagatt tgataaaggc cggcaggaca tcactgaacg agagattgtc
     9361 aaagacctgc gtccagaaaa gctggaaact ggtcaccccc ctgatattga ccccgaggta
     9421 agaaccatat aatgagatcg catcaccaag catcaccagt accggaagca tcaatgtggt
     9481 ggccaatacc cgggtaacca ccaggtattt gaacggattg gtgccagata cttccatggc
     9541 atcgatctgt tcggtcactt tcatagaacc cagttcagcg ccaatactac tgccgatttt
     9601 acctgcaaat attaatgcgg caatcaccgg acctatctcc cttacaattg cgatacccac
     9661 cattgcaggc aattgtgatt ccaccccata atcgatcagc gagggcctca gttgcatggt
     9721 cagcaccaat cccattataa aaccggtgag tccgaccagg ggcagtgatt tatagcctat
     9781 aataaaacat tgcctgaaaa attcagccac ttcatagcgg ggtctgaacc actggctgaa
     9841 aaagcgaccg gcgaaacggg acatatcacc agcctcatcg aaccagttct tccagttttt
     9901 aatctgcatt atttctgtac gcttactcat cttcataagc aacagggcct tcctccccgt
     9961 attcgtttct tttatataac ccgctgtatt ccccgaaagc cacaatatga ctggtcaccg
    10021 gcatattcca aagcagccaa tggacccagg taccaccggg agcatccgga tcttccttta
    10081 ttaatgccag gcaaaccgcc tcctccggta tatgatgaat gataaacggc ggactgatat
    10141 ttttcccatc gcaggtatat ctttcaggaa tcaatcctcc ttcggggaat gcggtgcact
    10201 ccacacgcaa ccgtttataa tcaaccgccc ttactggttc agaagttgtt gcattcatat
    10261 taaccggttc aaaaaattaa acaatcaggg accctcccca ggcgggggtg gaataaatgc
    10321 aggttccgtt tcttcttccg tttccggatc aataataata tcaggatcat cttccggcaa
    10381 ctgcagttct tcatcgccgg tatccggaat cgtatcgggc cgtttacccg gatacggaat
    10441 ttccggactt atgaggggtt taagattctt tgccatcgta cttaatttaa taaagtgatc
    10501 agactccaaa cgggtatgtt tccggtattt gtgtactatc cgtggccctt gcatcaccaa
    10561 tatgcgtatt gtgttcccaa accactactt tggcattcct tccatgaaaa tccaataatg
    10621 catcaagagt atccgccatg tgacggtccc tgacattcca gctatgtgga ccacctgtta
    10681 ccattgcccg gtaatatttt tctgcattca cagcaatccg tgcattttgc ccggcactga
    10741 aaacattttc agtatcggta ttgtaaccag ctgatttctc ctgtatagtt tttaacaagg
    10801 acactacttc ctgctgacac aactccggca caaactgtgc agccctggca taatcactac
    10861 cttccccttt ccggaaaggt tcgaagcagc gaaatgcttc ttcagcaatt ttcaatgctt
    10921 cgggatccgt ttttgagagg tattgcataa tattctccat actctcccat aaactgtaca
    10981 catccagtcc gtaaaaaccc acccgcttat tggccggcaa ttccgaatta aaccggtgca
    11041 gccattctgc taaagccacc atctcccaat tcgcccacat ccaggtaggc cagcggttaa
    11101 acacatgcaa tacgtcaaaa gcacttttac tagtcttatc cagtccttta acaaaacggt
    11161 tcagccggta gcaatccggc cagtcccctt caacagctat taactgaaag cccttctcct
    11221 ccaccagttt ccgggttata tatgcccgcc acgtgtaata ttcatgcgta ccatgactgg
    11281 cttcacccag cattacaatc tttgcatctc ctattttgtt aaacagtggt tcgaggtcct
    11341 ttttagtatt aagcgagcag gacctctgtt ttaataaatt aacggcctct gtttcgttaa
    11401 accgtgcaga ttgtgtgaaa taacgtcgca tggtgattta tcccctgttt ttgtgaacag
    11461 acggaatcct actcaaagca cgttctattt gccacctgtt taaaatgagg ccggtcactt
    11521 tcagatgtga tgatcatcat tggaaacagc tgctaaagaa aggtagattg tatctgctaa
    11581 acatattatt atgacaacca atgatacagc catctctaat cttcaccagt tactgaaatc
    11641 cgatatttgt cgtcaggcca gtgctgaagt gcagttacat aaggttttac ccggttgggc
    11701 cagcctggct acatcactga aactaaaggt cgttattcag aagtatcttg attttgtagt
    11761 attacaaaag caggaaatcg accagtttat tgaaatggaa aatatcaacc agttgccact
    11821 ttgtaaccgg gtaatgcagg cttttatcct tgatactgaa gaaaaagcag ggtattgcag
    11881 tgactccgaa gtaaaagatg cctgtttact tgcatctatc caggaaatca atcattataa
    11941 aatcagtgct tatggtacag ccgcggcttt tgccaataca ctggggatgg aagccaccgc
    12001 cctggttttc cgcaaagcag aggtaaacga aaaacaaatt gatgacaggc tttcacaact
    12061 ggcagaacat gagattaacc tgaaagcccg gaccccgatt gttttacccg gttaaaaaat
    12121 aaatcatgga ctggcgttta aatgatacag tcatcatacc ggtgcaaaat agctttgttt
    12181 acggcgaact ggaaattccg cttcatgctc aatcagttgt aattttttca catggcagtg
    12241 gaagcagccg gctgagcccg cgtaaccaat ttgtggcccg gtatcttcat caacagaata
    12301 tcgggaccct gttatttgac ctgcttactg aaaaagagga tcttaattat caaaaccgtt
    12361 ttgatataac cctactaaca aaacggctga tcagggcaac tgaatggctg gttgaacaac
    12421 cagtcgccag gcagctgagt ttaggttatt tcggagccag caccggtgcc gcttccgcaa
    12481 tcagggcggc ctccttactt ccgaatatac aggcggttgt ttcgagaggt ggacggcctg
    12541 acctggcggg cacagtttac ctgataaaac tggaagcccc gtctttgctt atagtcggaa
    12601 gtcttgatac tgaagtaatc cggctcaatg aatgggcggc ggctcagata aaagggaaaa
    12661 ataaactgga aattgttccg ggtgccaccc atctttttga agaagccggt actatggata
    12721 aagtttgtga actggctgcc gcctggttca ggcattacct gataccagtt gaagcctgac
    12781 ttaacaatcc gggaattttt tacaaataaa aaaatgcaac tatgttccgc aacagaaccg
    12841 atgccggtac ccggttagct gaaaaactga aaaaattcaa gaaggagccc ggtataataa
    12901 tggcagtacc caggggaggc gtaccggtag cctatgccgt ggccaaagaa ctgaacctgc
    12961 cggttgagct tgtactgaca aaaaaaatcg gccacccgct aaataaggaa tatgcgattg
    13021 gtgcagcaag tcttaccgat tattttatta ttccgcatga aggggtgact gatcattata
    13081 ttgaatctga attgcagcta atacgcagcc ggctgaaaga aatgcaaaaa acgctgcaag
    13141 cggatacgct tcctgaaaac tatgaaggga aaaccttact catcattgat gatggtattg
    13201 ctaccggcaa cacgatattg ggaacaataa aactgctgca caaaaacagg ccgggtaaaa
    13261 ttgtgatcgc tgcgcctgtt gcctcagaac ccgccgtgga aatgctggca aaagaagcgg
    13321 atgaagtgat cgtattactg atcccctctg aattttatgg tgtaggggca ttttatgaag
    13381 atttcaggca ggtgagtgat gaggaagtaa tgtattacct gaataaattg aaagaactca
    13441 ggaaagccgg ataataataa aacggagtta ccaaacaaaa ctccattcaa tgatcttaga
    13501 ataaaaaaaa attgtatacc atataaagcg ctgaaatccc acagtcttta ccctttattc
    13561 tggtggtttc atatcgcggc aggatccatc gatacggaca caggtttttt tccgggatca
    13621 ttgcggtgct acttcatgat gaatttcatt gcctgcctgc ttccacctgg gtcatgaata
    13681 accaggaaat aaaatccggc gcttaaccgg aggggtactg tgattatgtt tacggtttcg
    13741 tttccgatct taatatctaa ttctttcatc cttataccgg cctcattata aatccgcatt
    13801 tgataattcc ctttgggtac ggaagccatc tgcagttgta cctgcccggc ggagacgggg
    13861 ttggggaaaa tagaaaaacc ggtcaaggga ttctgactga caaacaaaac atggcttaaa
    13921 gtaaactttc catcccgatc gatcatttta agccggtaaa aactcatggc gggtaaaggt
    13981 tgttgatctg tccattgata gttaacaggc ccggtatcgc cggatgaaac aacaaatcct
    14041 atgctattaa atataaaccc gtcctgtgaa cgctgcaatt cgtaacctgc aaatgaatgt
    14101 tcatccttac ccgtccataa cagtacaaca gcgttaccct gtttctgcaa agtaaaatca
    14161 gtaagtataa gcggtaacaa ggcactacaa acgggtatgg cggcggtgac cccatcaacg
    14221 gaaatcgcgc cggtggttga gagtaatctg ccctcgagcg aactgctggc cgccatcgaa
    14281 acagccccgg gattggctat aaaattccct ttcattttac tcgatgcagc catcgctatg
    14341 gcccctcctt ctaccgccca gtaaatattg caggcaaggg ctccccctgt taatatgact
    14401 tgggatgaag gcccgggaga gaaagcacct tctattttta tgatgaaaat ggcattcgcg
    14461 tttcctcctg catccatgac aagatttcct tcaattgaag atgcttccgc aattaaatag
    14521 gttcccggta agagcgtctc gccattaccc aggatagcgg catgggtagg aaaagtctgt
    14581 tcggat
//
LOCUS       DAOPMW010000042        14468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4218_length_14468_cov_9.684035, whole genome shotgun sequence.
ACCESSION   DAOPMW010000042 DAOPMW010000000
VERSION     DAOPMW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14468
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_14468_cov_9.684035"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLU11_03830"
     CDS             complement(<1..247)
                     /locus_tag="PLU11_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN58273.1"
                     /translation="MMKFFRTIGFFLLFTFAFLLSSAQHEKYHAPSDTLVQKKLTQWQ
                     DLKFGLMMHWGTYSQWGIVESWTLCPEDEGWTVRRGPH"
     gene            complement(368..697)
                     /locus_tag="PLU11_03835"
     CDS             complement(368..697)
                     /locus_tag="PLU11_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58274.1"
                     /translation="MTLIPKELVPAINQSMEIELPATGTHEQLHEKLSVVINDLINHD
                     FEKLVFHLYRIDVDEARMRALLANKADQNAGGLIADLIIERQLQKIKAKAEFKQKAEE
                     GTGEEKW"
     gene            complement(702..1478)
                     /locus_tag="PLU11_03840"
     CDS             complement(702..1478)
                     /locus_tag="PLU11_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPN58275.1"
                     /translation="MKKNNLIEFLNEKVRLYNQPSFISEDPISVPHRFTKKQDIEIAG
                     FFAAIFSWGNRTTIINKSNELMKLMDEAPYAFCTGHTDNDLKRLLTFKHRTFNTTDLL
                     YFIEFFKWHYSKHKSLETAFTLHGETVEEMLTGFHHYFFSLEDAPPRTRKHIASPKKG
                     STCKRLNMFLRWMVRQDDKGVDFGIWKNIPPASLICPIDLHVARVANRFNLIYFKKTE
                     TLQVNWKTAVELTKYLRTLDPDDPVKYDFALFGLGVMEKF"
     gene            complement(1480..2619)
                     /locus_tag="PLU11_03845"
     CDS             complement(1480..2619)
                     /locus_tag="PLU11_03845"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HPN58276.1"
                     /translation="MKSATKYIHAGSHPDPSTGAIMTPVYMTSTYVQEAPGVNKGYEY
                     ARSQNPTRTALEEALAVIENGKYGLAFSSGVAATDAVIKLLNPGDEVICANDMYGGTY
                     RLFTKVFERFGIVFRYVDTTVASNVEAAITANTKLVWLETPTNPLMNITDIAAVAAIT
                     KAKKLILVVDNTFASPALQNPLDLGADIVMHSATKYLGGHSDVIQGALVMNDTELRDK
                     LYFIQKSCGAVPGPMDCFLVLRGIKTLGIRMKAHCENGKIIANWLRANPKVSKVYWPG
                     FEDHAGYAVAREQMKDFGGMISFELKNDSVEEAKRVLSSTHLFALAESLGGVESLINH
                     PASMTHASIPREERIKNGLSDSLIRLSVGIEDADDLVNDLAKAIG"
     gene            complement(2668..4518)
                     /gene="asnB"
                     /locus_tag="PLU11_03850"
     CDS             complement(2668..4518)
                     /gene="asnB"
                     /locus_tag="PLU11_03850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN58277.1"
                     /translation="MCGIAGIIQLPSAGYTKESLEKMTHALAHRGPDGEGYFQNHDST
                     VLLGHRRLAIIDRSDTGRQPMDYLGRYTIIHNGEIYNYKELKTELQQQGYSFHSQTDT
                     EVIVAAYDCWKEECLTRFDGMFAFAIWDEQKRELFAARDRFGEKPFFYYYDQQRFLFA
                     SEMKALWAAGIHKTPNQKMLFNFITIGYTDNPARPEETFYEGIQQLPPASYMKFSLVY
                     FQYSIKNWWELDPEIQDKEITEEEAIEEFSELFSASVRRRMRSDLPVGTSLSGGLDSS
                     SIVAAIHALHHTNNSQQCFTAVFDGFEKDESVFANTVARQYDLKQHLVRFTANDLLND
                     WDKLCAHQEEPFGSSSIYAQYKVYEKAREEGVTVLLDGQGADEILAGYQSYYKWYWQE
                     LFRKRKLYRSNELRYARERGIHENFDYRNMIAAYFPAYATVVLERHYLIKAIGHKDLS
                     PEFVQLQSKEAYYTPPEYFDLNGVLHFNTCIHGLSELLRFADRNAMAHGREVRLPFLD
                     HELVEFVFSLPSHFKIRQGWSKWLLRKSMDKKLPDSVNWRTGKVGFEPPQQQWMETKA
                     MQEGIREAKKILVEEKILQPGVLDKPNTPHSAHAADHYDWRYFSAASLFK"
     gene            complement(4561..5199)
                     /locus_tag="PLU11_03855"
     CDS             complement(4561..5199)
                     /locus_tag="PLU11_03855"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN58278.1"
                     /translation="MRLTAAILCCCLSLYSCQEGNKEEKKEPATSAIKDSAPSNRSER
                     PAVNPYNGVDVSPMDMVYYPVDYPKLKMAHTLSTPPLARVIYSRPHLQGRHIFHEVLK
                     YDEAWRLGANEATELTLYKDVTVQDKKIKAGRYTLYCIPHTDTWTIAINSNIDTWGLE
                     PDTARDVARFVVPVVRTQNSLEYFTMVFEKKEAEAQLLMAWDDVEVRLPLRF"
     gene            complement(5211..5771)
                     /locus_tag="PLU11_03860"
     CDS             complement(5211..5771)
                     /locus_tag="PLU11_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN58279.1"
                     /translation="MKKTLALLFFFQLFFLSLMAQTLPPVDKSPMDMSYYPANYPVLK
                     IQDKPTEALAARVVYSRPQKAGRTIYGGLVKYGEVWRLGANEATEIEFFKPVKIDGKK
                     VAKGRYTLYAIAYEATWTFILNKNTDTWGAFKYDPKKDILRVTVPAEKITETVESLAM
                     SFEKTTTGFKLVVAWDMVKAGLPISL"
     gene            complement(5847..6395)
                     /locus_tag="PLU11_03865"
     CDS             complement(5847..6395)
                     /locus_tag="PLU11_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPN58280.1"
                     /translation="MISDYVKGKKKLDYPTEIQWGIQLHRAIDQYTDTHEATREAKEV
                     FRPSYRLYSGAVVDVIYDHFLAADESEFTDDTLLQFARGVYEVLEHQQHLMPERFAGM
                     FPYMKEQNWLFHYRSRWGAGKSLGGLVRRAQYLTESETAFRLFEAHYQLLSDCYRHFW
                     NEVKPFAAQTFTELSGGENLTG"
     gene            complement(6483..7151)
                     /locus_tag="PLU11_03870"
     CDS             complement(6483..7151)
                     /locus_tag="PLU11_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPN58281.1"
                     /translation="MNKLINWLVALVILIPGIYLAFAWNGLPDKVPMHFDINGNADRY
                     GNKTELLTTILILPAVSLFVYFLLANIYRIDPKKYAVENRVRLKRIAFAIVCFLSALT
                     CIIIYSASHGGSKFDVSLILSAVGLLFAIIGNYLPNLKPNYFAGMRLPWTLENPENWR
                     KTHALAGKLWFAGGILLAITCLFLPSRAAMILFFSVMVILVIIPTVYSYRFYIQHKKS
                     GPVQ"
     gene            complement(7153..7416)
                     /locus_tag="PLU11_03875"
     CDS             complement(7153..7416)
                     /locus_tag="PLU11_03875"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPN58282.1"
                     /translation="MNSVFKALNDPTRREILELLQQRDMTAGEIVDQFTISGPSISHH
                     LDLLKQAGLVNAEKEGQYIYYSLNTTVVDEIMKWFLQFKTKKK"
     gene            7774..8211
                     /locus_tag="PLU11_03880"
     CDS             7774..8211
                     /locus_tag="PLU11_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58283.1"
                     /translation="MKKTLLALVVLISSLAVYAADENVNEKVLNAFKTEFTSASQVEW
                     TTGPNYYKASFVFNDKHVFAFYNIEGRLLGLTRNITSSELPLKLQTDLKKNYESYWIS
                     DLFEAAREEGTSYYLTLEDADTRLVLRASADNSWTVYEKRKKS"
     gene            8374..9348
                     /locus_tag="PLU11_03885"
     CDS             8374..9348
                     /locus_tag="PLU11_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPN58284.1"
                     /translation="MNKGPVSQFIQHHYRHFNSAALVDAAKGYEQHLLEGGKMMITLA
                     GAMSTGELGISLAEMIRQGKVDIISCTGANLEEDVMNLVAHSHYKRIPNYRDLTPQEE
                     WDLLENHYNRVTDTCIPEEEAFRRLQKHLVKQWKDAEAAGERYFPHEFLYKTVLSGDL
                     KQYYEIDPKDSWIIAAAEKNIPIVVPGWEDSTTGNIFASYVIKGELKASTVKNGIEYM
                     VWLADWYRNNSGGKGVGFFQVGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDINTPKFIVESDATIVAPLIFAWVLGW"
     gene            complement(9938..11482)
                     /locus_tag="PLU11_03890"
     CDS             complement(9938..11482)
                     /locus_tag="PLU11_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPN58285.1"
                     /translation="MFKKIMICVIALAPLLAAAQKKDKNAVKAAATITVEDLKKHLYI
                     IASKEMEGRETPSAGLDKAADYIEAHFKALGLTPGNNGSFRQFYPLYKDSMLSSSFKL
                     NGNAIELNKDYQPQTSNYAASMRFSEVVFAGYGISDPEKRDDYKDLKVAGKLVLIMDG
                     TPADYKPTQTGFASPASSFGKMNAALSKGASAVLIVYSNFPRRSLNANSGWSMNGYKA
                     TQTPFSFTISTEVAGKILGVDGKSLADKIKNGEIASKAYPAEIDLEFSKQSKMAKASN
                     VIGVLEGTDLKDEYVFITAHYDHLGKRDTVIYYGADDDGSGTTGVLEIAEAFIKAKEA
                     GKGPRRSIVFMAVSGEEKGLWGSGYYGNNPIFPLDKTTVDLNIDMIGRSDASRKVGDS
                     TNYVYVVGDDKISSDLKVISEGMNKQYVKMELDYKFNDPKDPNRIYFRSDHYNFAAKG
                     VPIIFYYDGMLGADYHKPTDTPEKIQYELFAKRAKLVFFTAWEMANRNEMLKRDIKLE
                     IPKRGM"
     gene            complement(11565..12020)
                     /gene="rpiB"
                     /locus_tag="PLU11_03895"
     CDS             complement(11565..12020)
                     /gene="rpiB"
                     /locus_tag="PLU11_03895"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPN58286.1"
                     /translation="MHSPFDLHKPIAIGSDHAGFKYKEQLISFLEGKGLPFTDFGTHS
                     ADSVDYPDFAHPVANAVENGEAAFGILICGSANGVAITANKHQGIRAAICWGEELAQL
                     SRAHNNANVICIPARFVREGDAEKMVAHFMTTAFEGGRHATRVNKISCG"
     gene            complement(12237..13091)
                     /gene="tatC"
                     /locus_tag="PLU11_03900"
     CDS             complement(12237..13091)
                     /gene="tatC"
                     /locus_tag="PLU11_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPN58287.1"
                     /translation="MAVFDFFNRRKTETQGEMTFIDHLEELRWHLVRSVIAVVICAIV
                     VMIFSSEIVEDVLMAPTRADFVSARWLCTMGQSIGIGDALCFTAVDAKFLENTMTGQF
                     LAYFTLGFMGGFVIAFPYIFWEFWRFVKPALSEKELKKTRGVIFWVSLLFFSGVAFGY
                     FILTPFMVNFYFNFKLSSQIVIMPSFSDYLENLIYTTVGIGVLFQMPLLVMVLARIGV
                     VTAKFLRKYRRHALVIIVIAAAIITPSTDPFSLTIVTIPLYLLFEASIMVAARINKQQ
                     EKAAKEWS"
     gene            complement(13345..>14468)
                     /locus_tag="PLU11_03905"
     CDS             complement(13345..>14468)
                     /locus_tag="PLU11_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPN58288.1"
                     /translation="NMEILSEFLESPARFLGTTLLGFNIFLVFLGLQISTVMQPIWSY
                     WHIGSDTVHIIVEIVLATFIVLIFAEFIPRAIFRARSNWLLQRLAQVTNVCYQLLFPI
                     AASFIDLSEWLLKYVFNIRMDKTNKEALKRSDLKHLFQSGNTDERQERNTQLMENAQE
                     LPKIKIRQCLVPRKEIVGIESKSTVEELTKKFVETKLSKLLVYENNIDHIIGYVHQLD
                     MFKKPETIQSVLLPIPAVPESMSATDLIGKFSKERKSIAWVVDEFGGTAGIITMEDVL
                     EELFGEIHDEYDTEEFVEKQLAEYEYIFSGRLELDYLEEKYGFVFGQEEAETLSGYII
                     NHHETIPRQKERIIIDDYEFDIIHVTDTRIETVKMKKLK"
BASE COUNT         3832 a         3390 c         3324 g         3922 t
ORIGIN      
        1 aatgcggacc ccggcgcacg gtccagccct catcttccgg acaaagggtc cagctttcca
       61 ctattcccca ctgactgtaa gtgccccaat gcatcatcag gccaaatttc agatcctgcc
      121 attgggttaa tttcttttga accagcgtat cggaaggagc atggtatttt tcgtgctggg
      181 cggaggaaag taaaaaggca aaagtaaaaa gtaaaaaaaa tcctattgtc cggaagaatt
      241 tcatcatcga aagatatttt atgacttaaa gtaaggaatg tgttcgattg ttcatataaa
      301 acaactagca cattcaatga tcagcaggat ctgactgcta tctgccgact gctatctgcg
      361 aactgattta ccatttctct tccccggtcc cttcctcagc tttctgttta aattccgctt
      421 tcgctttgat cttctgcaat tgtctttcaa taatcaggtc cgcgatcagt cccccggcgt
      481 tttgatctgc tttattggcc agcagggccc gcatcctggc ttcatccaca tcaatacggt
      541 acaggtggaa gaccagtttt tcaaaatcat gattgatcag gtcattgatc actacggata
      601 atttttcatg gagttgttca tgggtaccgg tggcggggag ctcaatttcc atggattgat
      661 tgatggcagg aaccagttct tttggaatta atgtcatatc gttagaattt ttccatgacc
      721 cctaaaccga ataaagcaaa atcatacttc acgggatcat ccggatccaa agttcgtaag
      781 tatttggtga gttcaactgc tgttttccaa tttacctgta aggtttctgt ttttttgaag
      841 tagatgaggt taaaccggtt ggctacccgg gccacatgca ggtcaatagg acagatcagg
      901 ctggccggcg ggatgttttt ccagatccca aaatccacgc ctttatcgtc ctgccttacc
      961 atccagcgca aaaacatatt cagccgcttg caggtggagc cctttttcgg actggcaata
     1021 tgtttccggg tacggggcgg ggcgtcttcc agggagaaaa agtaatgatg gaatccggtc
     1081 agcatttctt ctaccgtttc cccgtgcagg gtgaaagcgg tttcgaggct tttgtgttta
     1141 ctgtaatgcc acttgaaaaa ttcaataaaa tacagcaggt cggtggtatt gaacgtgcgg
     1201 tgtttgaagg tcagcagtct tttcaggtca ttatcggtgt gcccggtaca gaaggcgtaa
     1261 ggggcctcat ccattaattt catcagttcg ttcgacttat tgataatggt ggtccggttg
     1321 ccccaggaaa agatggccgc aaagaaaccg gctatttcga tatcctgttt tttggtaaaa
     1381 cgatgcggta ccgagatggg atcctcggaa ataaaagaag gctggttgta cagccttact
     1441 ttttcattta aaaattcaat cagattattc ttcttcatat tacccgatcg ctttcgccag
     1501 gtcatttacc aggtcatcgg catcttcaat gcccacgctc agccggatca atgaatcaga
     1561 taacccgttt ttaattcttt cttcccgggg tatcgatgca tgggtcatgc tggccgggtg
     1621 attgatcaga gattccacgc caccgagact ttcggccagg gcaaacaaat gcgtggagga
     1681 caagacccgc ttcgcttctt ccacactgtc gttctttaat tcaaaagaga tcataccgcc
     1741 aaagtctttc atttgctcac gggctacggc ataaccggca tgatcttcaa acccgggcca
     1801 gtatactttc gacacctttg gattggcccg taaccaattg gcaatgatct tcccgttttc
     1861 acaatgggct ttcatccgga tgccgagcgt tttgatgccc ctcaacacga ggaaacaatc
     1921 cattggaccg ggtacagctc cgcaactttt ctggatgaaa tataatttat cccgcagttc
     1981 cgtgtcattc atcaccagtg ctccctggat tacatcactg tgacccccga gatattttgt
     2041 agccgaatgc atcacgatat ccgcacccag gtccagcgga ttctgcagcg caggtgatgc
     2101 gaaagtattg tctacaacaa gaattagttt tttggctttg gtaatcgctg ccacggccgc
     2161 gatatccgtg atattcatca gcggattggt gggtgtttcc agccatacca gtttggtgtt
     2221 ggcggtgatg gccgcttcta catttgaagc cacggtggtg tccacatagc ggaatacaat
     2281 accaaagcgt tcaaatactt tggtgaaaag gcggtaggta cccccgtaca tatcgttggc
     2341 gcagatcact tcatcaccgg gattcagcaa tttgattacg gcatcggtgg cggccacccc
     2401 gctgctgaaa gcgagaccgt attttccatt ttcaatcacg gccagcgctt cctcgagtgc
     2461 ggtacgggtt ggattctgac tgcgggcata ttcataacct ttattcaccc cgggggcttc
     2521 ctgcacataa gtagaggtca tgtataccgg ggtcatgatg gcaccggtgg aaggatcagg
     2581 atgcgaaccg gcatggatgt atttcgtagc tgacttcata atagataata ttaaagtgaa
     2641 ttgaacagca aagatgacga aaagccctca cttgaagaga ctggccgccg aaaaataccg
     2701 ccaatcgtaa tgatcggctg catgggcact atggggtgtg ttgggtttat cgagaacccc
     2761 aggttgtagg attttttcct ctaccagtat ctttttggcc tccctgatcc cctcctgcat
     2821 agcttttgtt tccatccatt gctgctgggg tggttcaaaa cctactttcc cggtacgcca
     2881 gttcaccgaa tccggcagct ttttatccat actcttccgc aacagccact tactccagcc
     2941 ctgcctgatc ttgaaatggg aaggcaggga aaacacaaat tccaccagct catgatcaag
     3001 gaagggcaac cggacctccc gtccatgcgc catggcattg cggtcggcaa aacggagcag
     3061 ttcactgagt ccgtgtatac aggtattgaa atgcagtacc ccgttcaggt caaaatactc
     3121 aggcggggtg taataggcct ctttactctg caactggaca aattccggcg acaggtcttt
     3181 atgcccgatg gctttgatga gataatgccg ttcaagcacc acggtggcat aagcgggaaa
     3241 ataagccgca atcatattgc ggtaatcaaa gttttcatga atccctcttt cccgggcata
     3301 gcgcagttca ttgctgcggt acagttttct tttacgaaac agctcctgcc agtaccattt
     3361 ataataggat tgataacccg caagtatttc atcggcgcct tgtccgtcca gcaataccgt
     3421 caccccttct tcccgggctt tttcatacac tttatactgg gcatatatac tggaggagcc
     3481 aaagggctct tcctggtgag cacatagttt gtcccagtca ttgagcaaat cattggcggt
     3541 aaaacgaacc aggtgctgct tcaggtcata ttgacgggct actgtatttg caaaaacgga
     3601 ttcatccttt tcgaatccat caaatacggc cgtaaaacat tgctggctgt tattggtatg
     3661 atgtaaggca tgaatagccg ctacaatgga agaactgtcc agtcccccgc tgagtgaagt
     3721 accaacgggc agatcactcc gcatccggcg gcgcaccgat gcgctgaata attcactgaa
     3781 ctcttcaatg gcttcttcct ccgtaatttc tttatcctga atttcaggat ccagttccca
     3841 ccagtttttt atgctgtatt gaaaatacac cagcgagaat ttcatataac tggccggggg
     3901 taattgctga atgccttcat aaaaagtctc ctccgggcgg gcgggattat cggtataacc
     3961 gatcgtaata aaattgaaaa gcattttctg gttcggggtt ttgtgaatac ccgcggccca
     4021 gagtgctttc atttctgaag caaaaagaaa gcgttgttga tcgtaatagt aaaagaaagg
     4081 tttttccccg aatcggtcac gggcggcaaa gagttccctt ttctgctcat cccagatggc
     4141 aaaggcaaac atgccatcga aacgggtgag gcattcttct ttccagcaat cataagcagc
     4201 tacgatcact tcggtatcgg tttgggaatg gaaggaatag ccctgttgct gcagttccgt
     4261 ttttagttct ttataattat aaatttcccc gttatgaata atggtatagc ggcccaggta
     4321 atccattggt tgacggccgg tatcggagcg gtcaatgatc gcgagccggc ggtgtccgag
     4381 cagaaccgtg gaatcatgat tttgaaaata tccttcccca tccggaccac gatgggccag
     4441 ggcatgggtc attttttcca atgattcttt cgtgtaaccc gctgagggta gttgtataat
     4501 accggctatg ccacacatac tgtaaatatc tgtaataatt acggcagtag ccgaaaagga
     4561 tcagaaacgc aaggggagcc ttacttccac atcatcccag gccatcagca attgtgcttc
     4621 ggcttccttc ttttcaaata ccatggtaaa atattcgagg gagttctgtg tccggaccac
     4681 cggcaccaca aaacgggcca catcccgggc cgtatccggc tccagtcccc aggtatcaat
     4741 attgctgttg atggcaatgg tccaggtatc ggtatgcgga atgcagtata aggtgtaacg
     4801 gccggctttg atttttttat cctgaaccgt gacgtcttta tacagcgtca gttctgtggc
     4861 ttcattagca cccagcctcc aggcttcatc gtatttcaat acttcatgaa atatatggcg
     4921 gccctgcagg tgcggacggc tatagatcac ccgggcaagg ggaggcgtac tgagggtatg
     4981 cgccattttt aatttcgggt agtccaccgg atagtacacc atatccatag gggatacatc
     5041 taccccgtta taaggattaa cagccggtct ttcggaacgg ttggaaggcg cactgtcttt
     5101 gatggcagaa gtcgccggct ctttcttttc ctctttattc ccttcctgac aggaatagag
     5161 ggaaagacag caacataaaa tcgcggcagt taatctcata agaaagcaat ttataaactt
     5221 attggcaaac cggcctttac catatcccag gccacgacca gtttaaagcc ggtggtggtt
     5281 ttttcaaaac tcatagccag cgactccacc gtttcggtta ttttttcagc cggaactgtc
     5341 acccgcaata tatctttttt agggtcatat ttaaaagcac cccaggtatc tgtgttctta
     5401 ttcaggataa aggtccaggt ggcctcatag gcaatggcgt aaagggtata gcgtcctttg
     5461 gctacttttt tcccgtcaat tttcacgggt ttgaaaaatt caatttcagt ggcttcattc
     5521 gcacccagcc gccagacctc cccgtattta acgagtccgc cgtatatggt acgacccgct
     5581 ttctgcggac ggctatatac tacccgcgcg gccagggctt cggtgggttt gtcctggatc
     5641 ttcagcaccg ggtaattggc cgggtaataa ctcatatcca tcggtgattt atccaccggg
     5701 gggagggttt gggccatcag ggacaaaaag aacaattgaa aaaagaacag gagggcgagt
     5761 gtttttttca tggactattt tttaaaaggc cgcaacgggt tgcggataac aaatgtaaaa
     5821 cgaattgcca gtccggatgt taccttttat ccggttaaat tttccccgcc ggacagttct
     5881 gtaaaagtct gagcagcaaa aggtttgact tcgttccaga aatgacgata acagtccgat
     5941 aatagttggt aatgggcttc aaaaagccgg aaagcggtct cgctttcggt caggtactgg
     6001 gcacgccgta caagtccgcc caggcttttc ccggctcccc atcggctccg gtaatgaaaa
     6061 agccagtttt gttccttcat ataaggaaac atacctgcaa agcgttcggg cataagatgt
     6121 tgctgatgtt ccaggacttc atagacaccc cgggcaaact gaagaagcgt gtcatcggtg
     6181 aattccgatt catcggctgc cagaaaatga tcatagatca catccaccac ggcgccgcta
     6241 taaagtcggt atgaaggccg gaatacttcc ttggcctcgc gggtggcttc atgggtatcg
     6301 gtatactgat caatggcccg gtgcagttgt ataccccact gaatttccgt ggggtaatcc
     6361 agtttctttt ttccttttac ataatcactg atcatattgc ccaccagtac acccggatgg
     6421 ttgaaagaga gatatgcgtg tgcgaggtaa ttgataaggc tattcgttta taaagcacaa
     6481 atttactgaa ccggtccgct ttttttatgc tgaatataaa agcggtagga ataaacggtt
     6541 ggaattatta cgagaatcac cattacgcta aagaaaagga tcatggctgc ccgtgatgga
     6601 agaaaaaggc aggtaatggc gagcaggatt cccccggcaa accagagttt tcctgcaagg
     6661 gcatgcgtct ttctccagtt ttcggggttt tcgagtgtcc agggcagacg cattccggca
     6721 aaataattag gtttcagatt gggcaggtag ttgccgatga tggcaaataa taaaccaact
     6781 gctgacagga tcaggcttac atcaaatttt gaaccgccat gggaggcact gtaaatgatg
     6841 atacaggtaa gtgcggaaag gaaacaaaca atcgcgaaag cgatcctttt caggcgcacc
     6901 ctgttttcca ccgcatattt tttagggtcg atccggtaaa tattggccag caggaaatac
     6961 acaaacaggc taacagcagg taaaataaga atggtagtca gcaattctgt tttattacca
     7021 taacggtctg cattcccgtt gatatcaaaa tgcatgggca ctttgtcggg cagaccgttc
     7081 caggcaaagg ccaggtaaat acccggaatc aggatgacga gggccaccag ccaattgatc
     7141 agcttattca tcttatttct tttttgtttt gaattgtaaa aaccatttca tgatttcatc
     7201 caccaccgta gtgttcaatg agtaatatat atactgtcct tctttttccg cattcaccag
     7261 ccctgcctgt ttcaacaggt caagatggtg ggaaatactg gggccggata tagtaaactg
     7321 atccacaatc tcaccggctg tcatatcccg ctgttgcaac aactcaagaa tttcccggcg
     7381 ggtaggatca ttcagggctt taaataccga gttcatcagg gttaattata tatttagaca
     7441 aatatctaaa tactttttct tttgaacaaa taaattgtcc gtattcttta ttcctacagg
     7501 ataaccggta tttataaatg ctggacggtt aataagatat agttcctgtt aacagctatt
     7561 aacatcggct tccgttaatc ataattggct tgtgaacggc tgtaaccaat tctatctttg
     7621 aaccgtcaca cagatacagg aatagtcgtt agtcaatagt cgttagtcaa tagtcgatag
     7681 tcattagtaa acagccaata cacttattaa gaatataacc ttttgaactt tttaaacctt
     7741 ttaaacctct tgaacctctt aaacctttca aaaatgaaaa agacgctgtt ggcccttgtg
     7801 gtactgatca gttccctggc cgtatatgcc gctgatgaaa atgtcaatga aaaagtattg
     7861 aatgccttta aaacagaatt cacatcggca agtcaggtgg aatggaccac cggccccaat
     7921 tattataagg catcctttgt attcaatgac aagcatgtat ttgcttttta taatattgaa
     7981 ggccgtcttc ttggactgac ccggaatatc acgtcatctg aactgccgct gaaactgcag
     8041 acagatctga aaaaaaatta tgaaagctac tggatctctg atttgtttga agccgcccgc
     8101 gaggaaggaa catcttatta cctgaccctg gaagatgcgg atacccgcct ggtgctcagg
     8161 gcttctgcgg ataacagctg gacggtctac gaaaagagga aaaaatcctg aacgacttag
     8221 ttttggtgtg tggtaaaaca accctggtct gcgtctgcag ccggggtttt gtttttacta
     8281 tattttcagc aaaacagtac ttttgcgcac cgcaatgtct catcccgtac gatacgggat
     8341 gatctgaagg cggatcttaa aactataatt aaaatgaaca aaggtccggt ttctcaattc
     8401 attcagcatc attaccgtca cttcaactcg gccgcattag tagatgccgc caaaggctat
     8461 gaacaacacc tgctcgaagg gggtaaaatg atgatcaccc tcgctggtgc catgagcaca
     8521 ggcgaactgg gtatttccct cgccgaaatg atccgccagg gaaaagtgga tattatcagt
     8581 tgtacagggg ctaacctgga ggaggatgtc atgaacctcg tggcacactc ccattacaaa
     8641 cgcattccca attaccggga tctgacgcca caggaagaat gggatctgct cgaaaatcac
     8701 tacaaccggg taaccgatac ctgtattccg gaggaagaag ctttccgtcg tctgcaaaag
     8761 cacctggtaa aacagtggaa agatgcagaa gcagccggag aacgctattt tccgcatgag
     8821 tttttataca agaccgtact gagtggcgac ctgaagcagt attatgagat agatcccaaa
     8881 gacagctgga tcattgccgc cgctgagaaa aatattccga tcgtggtacc gggctgggaa
     8941 gacagcacta ccgggaatat attcgccagt tatgtgatca agggcgaact gaaagcttcg
     9001 accgtaaaga atggcattga gtatatggta tggctggccg actggtaccg gaataattcc
     9061 ggtggcaaag gggttggctt cttccaggta gggggcggta ttgccggaga tttcccgatc
     9121 tgtgtggtgc ccatgatgta ccaggacctg gaatggcatg atgtgccttt ctggagttat
     9181 ttctgccaga tcagtgattc caccacttcc tatggctctt attcaggggc ggtgcccaat
     9241 gaaaagatca cctggggtaa gctggatatc aatacgccca agtttatcgt ggaaagcgat
     9301 gccaccatcg tggcgccatt gatttttgcc tgggtactcg gttggtaaaa aatcaaaaca
     9361 gcgtataatg gaagaacccc gaacaacgag ttgtacgggg ttcttgctat tttgaaggta
     9421 gggattaaaa atgctgtcag gccaacatat ctatcagaca gatatcagac cccggaagtt
     9481 gcgttaggtt taaaaaaaag ttgactgcgg caatatttat aggccacaaa aacacacgaa
     9541 gagacacgaa aaaatacaat aaatacaagc cacggataac acagatatac acggatttga
     9601 ttgcctgcgg caattttttt gccactaaag cacactaaag ggcacgaata aatacaagcc
     9661 acggataaca cagatataca cggatttgat tgcctgcggc aattttttgg ccactaaaac
     9721 acacgaaagg gcacgaaaaa tacaatacat ggatctacct ttctgtatta ttcgtgccct
     9781 ttcgtgttta ttcgtggcca cccgctttgc cgcaggcaat ccgtgtatat ccgtgcaatc
     9841 cgtggcctgc ctttatgtat tcttcgtgtc attttcgtgt tcattcgtgg caaaaaaaat
     9901 ccccggcttg ccggggattt caaagcattt ggggcgatca catgccgcgc ttcgggatct
     9961 cgagtttgat atcccttttc agcatctcat tccggttcgc catttcccag gcggtaaaga
    10021 ataccagctt cgcccttttt gcaaaaagct catactggat tttttccggc gtatccgtgg
    10081 gtttatgata atccgccccc agcatcccgt cataatagaa aatgatgggt acacccttgg
    10141 ccgcgaaatt gtaatgatcg ctgcggaaat aaatacggtt cggatctttc ggatcattga
    10201 atttataatc cagttccatt tttacatact gcttattcat gccttcactg atcactttca
    10261 gatcactgct gatcttatca tcacccacca catatacata attggtggaa tcccccactt
    10321 tacgactggc atcactgcga ccaatcatat cgatattcag gtccacggtg gttttatcca
    10381 acgggaaaat gggattgttg ccgtaataac cagaacccca taatcctttt tcctcaccgc
    10441 tgacggccat aaatacaata ctgcggcggg gtcctttgcc ggcttctttg gctttaataa
    10501 aagcttccgc aatttcaagt acccctgtgg tgccggaacc atcatcatcc gcaccatagt
    10561 agataacggt atcgcgttta cccaaatgat cgtagtgagc ggtgataaat acatattcat
    10621 ctttcaggtc cgtgccttcc agtacgccaa ttacgttgga agctttcgcc attttggatt
    10681 gcttgctgaa ttccaggtcg atttcggcag gatacgcttt ggaagcgatc tccccgtttt
    10741 tgattttatc ggccagactt ttgccatcca cgccgaggat ctttccggct acctctgttg
    10801 aaatggtaaa actgaatggg gtttgcgtcg ctttgtaacc gttcatactc cagccactgt
    10861 tggcattcag gctgcggcgg ggaaaattgg aataaactat aaggaccgcg gaagcgcctt
    10921 tgctcagggc ggcattcatc ttgccaaaag aactggcggg agaagcaaaa cccgtttggg
    10981 tgggtttata gtctgccgga gttccgtcca taatcaggac cagtttaccg gccactttca
    11041 ggtctttata atcatcgcgt ttttccggat cgctgatgcc atatccggca aacaccactt
    11101 cagagaagcg catggaagcg gcgtagttac tggtctgtgg ctggtaatcc ttattgagtt
    11161 caatagcatt gccatttaat ttaaaggaac tgctcagcat agagtcttta tacaacgggt
    11221 aaaactggcg gaaactgccg ttattccccg gggtcagtcc cagggctttg aaatgcgcct
    11281 caatataatc cgctgcttta tccagtccgg cagaaggggt ttccctacct tccatttctt
    11341 tactggcaat aatgtacagg tgtttcttca ggtcttccac ggtgatcgtg gcggcggctt
    11401 ttaccgcatt tttgtctttt ttctgtgcag cggccagcag gggagccagg gcgatcacac
    11461 agatcatgat ttttttaaac atatgggtgt tattggtttg tagcaggggt aaaaataatc
    11521 ccttccggga aaggaaggga taaaatatgg atgaccggtt ttaatcagcc gcaagagatc
    11581 ttgttgaccc tggtggcatg tcgtcctcct tcaaaagccg tggtcataaa atgagcgacc
    11641 attttctccg catcaccttc acgtacaaag cgggcaggta tgcagatcac attggcatta
    11701 ttatgtgccc gtgacaactg ggccagctct tctccccagc agatcgccgc cctgattccc
    11761 tggtgtttgt tggccgtaat ggcgacaccg ttggcactgc cacagatcag aatgccaaat
    11821 gccgcttccc cgttttcaac tgcattagct acgggatgtg caaaatccgg ataatccact
    11881 gaatcagcac tatgcgtacc aaaatccgta aacggtaaac ctttcccttc caggaacgaa
    11941 atcagttgct ccttatattt aaatccggca tgatcactgc cgatggcgat gggcttgtgc
    12001 aggtcaaagg gagaatgcat aagaagaagt tgaataaaag atgaaatagt tactgcccgg
    12061 taaagttacg aatctataaa caaagtacga ggtacgaagt acgaagtacg gaaagcagaa
    12121 agagtcttat attattgaaa taatttaaag caggtaaagg ctccattctt acagattttt
    12181 cctgtcagga tgggctgttg actcccgact actaactact aacttccgac tagttatcag
    12241 ctccattcct tcgccgcttt ctcctgttgc ttattgatcc tcgctgccac cataatactg
    12301 gcttcgaaga gcaggtagag cggaatggtc acaatggtca ggctgaacgg gtctgtggag
    12361 ggggtaatga ttgcggcggc aattacgatg atcactaatg cgtgacgacg gtatttgcgc
    12421 aaaaattttg cggtaaccac accaatgcgg gccagcacca ttaccaataa gggcatttga
    12481 aaaagtacac cgattcctac agtggtataa atcaggttct ccaggtaatc ggaaaaagaa
    12541 ggcatgatca caatctggga actgagtttg aagttgaaat agaaattcac catgaacggc
    12601 gtcagaataa aatagccaaa agccacgccg gaaaagaata agagagagac ccagaaaatc
    12661 actccgcgtg tttttttcag ttctttctca gataaagcag gcttgacaaa acgccagaat
    12721 tcccagaaga tatagggaaa ggcgatcaca aaaccgccca taaatcctag ggtgaaatag
    12781 gccaggaact gaccagtcat tgtattttca agaaatttgg catccaccgc ggtaaaacaa
    12841 agcgcatcac cgattccgat actttgtccc atggtacaaa gccagcgggc agacacaaaa
    12901 tcagcccggg tgggggccat cagcacatct tccacaattt cacttgagaa gatcatgact
    12961 acaatagcac agattaccac ggcaatcacc gacctcacca ggtgccagcg caattcctcc
    13021 aggtggtcga taaatgtcat ctctccctgg gtttccgtct tccgtcggtt aaaaaaatcg
    13081 aatactgcca tggtttttat tacgagaggc aaatatagct aattagccaa tgtgcaagag
    13141 agagaatgtg ccaattagcc aatggacgta ctaaggaaat tgtcttcaat gatgaatatt
    13201 atatgtatta agcaggtcat cgtcatcatc cgggcaaaaa gtgtaactag atgttttagc
    13261 ttccttattt accacctgag agcaattttc ggtctttctt actcttcccc aattgacaca
    13321 ttggctaatt ggcatattgg cacattactt tagcttcttc atcttcaccg tctcaatcct
    13381 tgtatccgtt acatggataa tatcgaattc gtagtcgtcg atgatgatgc gttctttctg
    13441 acgggggatg gtttcgtggt gattgatgat ataaccggag agggtctcgg cttcttcctg
    13501 gccaaagaca aagccgtatt tttcttccag gtaatccagc tccaggcggc cggagaaaat
    13561 atattcatat tcagccagct gtttttcaac aaactcctcg gtgtcatatt catcatggat
    13621 ctctccaaat aactcctcca gtacatcttc catggttatg attccagccg taccaccaaa
    13681 ctcatccacc acccaggcaa tactcttccg ttccttgctg aattttccga tcaggtcagt
    13741 ggcgctcatg ctttcaggta cggcgggtat gggtaacaag accgactgga tggtttccgg
    13801 tttcttaaac atatccaact ggtgtacgta gccaatgata tgatcgatat tattttcata
    13861 gaccagcagc ttactgagct tggtctccac aaatttttta gtaagttctt ccacggtaga
    13921 cttgctttcg atgcctacga tctctttccg gggtaccagg cactgacgga tttttatttt
    13981 gggcagttcc tgtgcatttt ccatcagctg ggtattgcgc tcctgccgct catcggtatt
    14041 gccggactga aacaggtgct tcagatcact ccgcttcagg gcttccttat tcgttttatc
    14101 catacggata ttgaacacat atttcagcag ccactcagaa agatcaataa aggaggcagc
    14161 gatgggaaac aataactggt agcacacatt ggtcacctgg gccagccgct gcaataacca
    14221 gttactccgc gcccggaaaa tggccctggg aataaactct gcaaatatga gtacgataaa
    14281 agtggcgagg acaatctcta ctatgatgtg aaccgtatca gaaccgatat gccagtaact
    14341 ccagatcggt tgcatcactg tgctgatctg caatccgaga aagaccagga aaatattaaa
    14401 acccagtaaa gtggtgccca ggaaacgggc cggtgattcc aggaattcgg agagaatctc
    14461 catattac
//
LOCUS       DAOPMW010000043        14158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4349_length_14158_cov_10.398426, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000043 DAOPMW010000000
VERSION     DAOPMW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_14158_cov_10.398426"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1019)
                     /locus_tag="PLU11_03910"
     CDS             complement(186..1019)
                     /locus_tag="PLU11_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPN58289.1"
                     /translation="MNELTAAASQVRRDIVRMVHGASSGHPGGSLGCADFLTALYFRI
                     MKRKPGFDMDAREEDVFVLSNGHISPLFYSVLARTGYFDVKELATFRKLNSRLQGHPA
                     THEHLPGVRIASGSLGQGMSVAIGAALTKKMNGESNLVFSLHGDGELNEGQIWESVLF
                     AAHHKVDNLISTIDWNGQQIDGPTDKVMSLGNLRQKFDAFGWQTLEMNGNDMDEVVLT
                     LEKAKSLTGQGKPIAILMHTAMGRGVDFMEGTHEWHGIAPNDEQLTKALAQLPETLGD
                     Y"
     gene            1258..2397
                     /locus_tag="PLU11_03915"
     CDS             1258..2397
                     /locus_tag="PLU11_03915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPN58290.1"
                     /translation="MLDILLTASVSFIITFLAVPVVLEVAEKKKLFDIPNERKVHTRL
                     VASLGGVGIFGGFILAALLSIQGHLNYEFQYFFAASLVIFFLGLKDDLIILSASKKFI
                     GQIIAASILIHLGGIRLDSMHGILGFEKLPEAFSLALSYLTIIVVINSFNLIDGVDGL
                     AASLGILTMLVFGSYFFAIGYQAYALLAFAMAGSLAAFMIFNHHPARIFMGDSGSLMI
                     GLINAILVIKFINVANDPLVAVPVPSAVAIGFGILIVPLLDTLRVFSIRILNGQSPFT
                     PDRNHVHHLLLDRGMDHAAVTLTCVSVNIGFIILAWFGRHLGPNYLMLLMLTLAFSGL
                     GLLYYKRPRRAVLIAKRSDGVATMQTKGTKVVTLTKEKEAQSAVD"
     gene            2532..3095
                     /gene="frr"
                     /locus_tag="PLU11_03920"
     CDS             2532..3095
                     /gene="frr"
                     /locus_tag="PLU11_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPN58291.1"
                     /translation="MSEELSSIHSGAEEHMKKAISHLETELVKIRAGKANPQMLDGIM
                     VDYYGSPMPIGQVANVNVMDARTLSIQPWEKNMLQPIERAIIAANIGINPQNDGVLIR
                     MFLPPLTEERRKELVKKCQGEGEQSKIAIRNIRRDAIEHIKRLQKNGLSEDVAKDAET
                     GVQEMTDKFISTVEKHLASKEKEIMSI"
     gene            complement(3125..4036)
                     /locus_tag="PLU11_03925"
     CDS             complement(3125..4036)
                     /locus_tag="PLU11_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN58292.1"
                     /translation="MNSKLISWALFIALCVIWGSSFILMKWGLYDSNQQPVLSAYQVA
                     ALRMLSSGLVMLPFLKRSLKNLPAPTLRYVLLSGWLGSFFPAFLFCIAETKIDGALTG
                     SLNSLTPIFVIITGALFFRKQTSWTKISGVLVGLVGSALLLYINAGKPNGYLAYTGFV
                     VLATIFYGFNVNMVHERLHGVSSVQIATVAFTGLIPPSLIILIVTGYFNLPLTEHVYT
                     MSTIASTLLGILGTALASVLFYVLVKKAGGLFASLVTYGIPFIAILWGVYYGEKVTGM
                     QVIALGIILLGVYLANRPETKKATPVE"
     gene            4229..4975
                     /locus_tag="PLU11_03930"
     CDS             4229..4975
                     /locus_tag="PLU11_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HPN58293.1"
                     /translation="MSKVKLSSLDPRAPKDLDKQDTKAATEKILVELDELQNLLYAEG
                     KHSVLIVIQGMDGSGKDGVIRNVLGNMNPQGVMVKSYKAPTAEELSHDFLWRIHHHAP
                     AKGMIQVFNRSHYEDILVTRVHKWCDDETARKRMAAINDFERLLQEHNNTHILKFYLH
                     VSPEEQHERLSERLKEPAKMWKYNEKDFDEAKLWDIYMEMYEDCFAHCNKPEWTIVPA
                     DQNWYKESIIARALRDLLKGLDMQFPGMKK"
     gene            5049..5417
                     /gene="mscL"
                     /locus_tag="PLU11_03935"
     CDS             5049..5417
                     /gene="mscL"
                     /locus_tag="PLU11_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPN58294.1"
                     /translation="MGMLKEFRDFAMKGNIVDLAVAVVIGGAFGKIVDSMVGDIIMPI
                     LGKIMGDPNYFLTWTAAGIPVGKFIQAIFNFLIIAFVLFLVIKAMNSVKKKEEAAPAA
                     PAPTPEDIQLLREIRDALKK"
     gene            5521..6306
                     /locus_tag="PLU11_03940"
     CDS             5521..6306
                     /locus_tag="PLU11_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPN58295.1"
                     /translation="MQNPSYQIKLPQFEGPFDLLLFFIERDELDIYNIPITRIINDFM
                     DYIHQSGELNIELSSEFILFISTLMRIKAKMLLPRKELDAQGNEIDPREELVNKILEY
                     KRFKEASAEMAEMEAVRMLMVKRGNIQKELSQIGEEDSEGTEIQNITLFKLMKSFEKA
                     MQKYSDRLNKPVHTVVSYNYTMEQSRDEMLSLAREEKTIAFERIFERSANRVQAIFLF
                     LSLLELVQQKFLKIMIGEGKNNFIIEFIEPDERLTELDEQNQL"
     gene            6334..6744
                     /locus_tag="PLU11_03945"
     CDS             6334..6744
                     /locus_tag="PLU11_03945"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPN58296.1"
                     /translation="MKSFATANWKGTGKEGNGTLSSKSKVLENTSYDFRSRFEDGTFT
                     NPEELVAAAHAGCFSMKLSFVLGAAGFTPDNIDTKCTITLEAGAVTNSHLEVTASVPG
                     IDAAKFAECAADAKANCPISKLLNTEITMEATLA"
     gene            6812..7495
                     /locus_tag="PLU11_03950"
     CDS             6812..7495
                     /locus_tag="PLU11_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58297.1"
                     /translation="MKILFLALLLTCNLILYAQQTSAGMNGTQRRMEVYSWAERPEEK
                     VEYDLQKMFLDQQWMPGIVSFKSGRPAMAVPLILDLHNNTLYYLQGAVIMEFVDTVSQ
                     FLIRVPSKKDSILMKFKCQLPAIQTNNSETFYEVLVDGDIKLLKCRAKSILLFKDKNL
                     PEEIKQLYFAELPGNKLVMIPMDADGIKEKLPDYNAEITAILKKEHIKLKNENKLAEL
                     FVHLNNTQH"
     gene            complement(7569..9503)
                     /gene="dxs"
                     /locus_tag="PLU11_03955"
     CDS             complement(7569..9503)
                     /gene="dxs"
                     /locus_tag="PLU11_03955"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPN58298.1"
                     /translation="MQITPGPLLQTIDSPDDLKKLPREKLHQLCDELRQYIIDVVSVH
                     GGHFGASLGVVELTTALHYVYHTPYDQLVWDVGHQAYGHKILTGRRDNFPSNRKYKGL
                     SGFPKRSESEYDTFGVGHSSTSISAALGMAMAAKYKGEKDRKVVAVIGDGSMTAGLAF
                     EGMNHAGVADSDMLIILNDNGIGIDPNVGALKEYLTDISTSPTYNKVKDDVWKLMGKL
                     PVGKNFSRAMAHKLIEGMKGMVSSSANLFEALNLRYFGPIDGHNVAKLVDTLRDLREI
                     PGPKILHVITTKGKGYALAEQDQTKWHAPGLFDKLTGEIQKKKFDIPQPPKYQDVFGH
                     TMIELAEQNDKIFGITPAMPSGSSLKFMMEKMPDRAFDVGIAEQHAVTLSAGMATQGL
                     KVFCNIYSSFMQRAFDSVVHDVAIQKLPVVFCLDRAGLVGDDGPTHHGCYDIAYFRCI
                     PNLVICSPMNESELRNLMYTAQLESTKLPVVIRYPRGEGMMPEWKTPMKEIEIGKGRK
                     LRDGQDIAVLSFGHPGNFAAAAIRDLKNDGLNPGHYDMRFAKPLDEALLHEACQRYEK
                     LLTVEDGTVEGGFGSAILEFMNRHGYKNEVRILGIPDRLVEHGTPKELIRECGYDAEG
                     IASSIREMMKEKITVSTLLG"
     gene            9705..10772
                     /locus_tag="PLU11_03960"
     CDS             9705..10772
                     /locus_tag="PLU11_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN58299.1"
                     /translation="MWFANSRYWLFQAVGWGGFTLISIFFAYSFDQLNTREQLTQVFG
                     RLGIFVILGIMLSHLMRSLILNVDLLQKKLERQFLYFLLITLLFSVIGSLIYMQVLQH
                     FNLLNKREHEFLDNKVLLIASGVFNFFVYFFIWNLIYFIYHYVTKSRKQQLDTLRLES
                     MVKELELKTIKAHINPHFIFNSLNSIRALVDENPQRARNAVTELSNILRSSMQTEQLE
                     TVPLERELGIIKDYLALENMRFEDRLKVEYDIDEDTLNQPVPPMMLQTLVENAIKHGI
                     SKQINGGLVKVISAFKGDYHELVVQNTGQLDGRQPTEEGFGLSSTTNRLSLLYGQKAS
                     FEIKQINGSLVEARVQIPVQV"
     gene            10806..11534
                     /locus_tag="PLU11_03965"
     CDS             10806..11534
                     /locus_tag="PLU11_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN58300.1"
                     /translation="MKAIIIDDERLARLELRKMLLEFPEVEVIDEAVNAEDALAKIEA
                     LQPDLIFLDIQMPGKTGFDMLAELDKAPQVIFTTAHHEFALKAFEVNALDYLMKPVEP
                     KRLADAIQKLQQSEGREQRGETEFVNNSILSEHDQVFVKDGERCWFVKLSEIRLFESV
                     GNYAKVYFGANKPLILKSLNALEERLDEKVFFRANRKHIVNLRMIDKIEPYFNGGLLL
                     DLKGGEKIEVSRRQTVKFKEMMSL"
     gene            11794..13080
                     /locus_tag="PLU11_03970"
     CDS             11794..13080
                     /locus_tag="PLU11_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN58301.1"
                     /translation="MIRQSIAIILFTASGLSLAAQKGFDIINVKEVERIEKTLASDDM
                     RGRRTFTPDIDKAADFIAAEFKAIGLGTMNNTGSYRQEFAMVRPKFISATAMLDGQVV
                     EQKSVIVITCQPQLKIDQTAGYETAFIKTGTNLQTEARKYARGNKNMIVWVEKSFANS
                     FGNLARLKSSMFKTATSVVFVLTDTAPAAYTIEAVHEIIEQKMANVVGVIPGKSMVNE
                     FVVFSGHYDHLGVGKPVDGDSIYNGANDDAAGTTAVIMLAKYFKKLGNNERTLVFAAF
                     TAEEVGGFGSQYFSRQLDADKVMAMFNIEMIGTESKWGKNSAYITGYEKTNMGDILAK
                     DLQGTAFTFYPDPYPTQQLFYRSDNATLARLGVPAHTISTSKMDVEPHYHKLTDHIET
                     LDMANMTEVIKAIALSSKSIISGKETPTRVKVEELR"
     gene            complement(13566..>14158)
                     /locus_tag="PLU11_03975"
     CDS             complement(13566..>14158)
                     /locus_tag="PLU11_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPN58302.1"
                     /translation="ISNHLDECVQQIKKSCDIPVILFPGSPSQVSKYADALLYLSLIS
                     GRNPELLIGQHVVSAPFVKKSGLEIMPTGYIVVDGGAPTTVSYISNAAPVPADKNEIA
                     MCTAMAGEMLGMKLIYMDAGSGAKRPITESMIKAVADHIEVPLIIGGGILQPEKAYLN
                     CKAGADVIVVGNAIEKDASLIKEMSDAVHSVPVVTG"
BASE COUNT         3891 a         3262 c         3349 g         3656 t
ORIGIN      
        1 attttctcct ggtcggagct accgttttcc tgatagcttt tcttgcttcc tggataccct
       61 cacgcaaagc ggcttcgcag gagttttcgt tgcggacgga gtagggtaca attgaaaatt
      121 gagcattgaa aattgagtat tgaacatttc tctttgaaaa atgctcaata ctcaatgctc
      181 aattatcaat aatcccccaa ggtctccggc aattgtgcca gcgccttggt cagttgctca
      241 tcattcggag caataccatg ccattcgtgg gtgccttcca taaaatcaac cccacggccc
      301 atggccgtat gcatcaggat ggcaatcggt ttgccttgac cggtcagtga ttttgctttt
      361 tcaagggtca ataccacttc atccatatca tttccgttca tttccagggt ctgccagcca
      421 aatgcgtcaa atttctgacg gagattaccc aggctcatta ctttatcggt gggaccgtcg
      481 atctgctggc cattccagtc gatcgtactg atcaggttat ccactttatg atgggctgca
      541 aataaaacgg attcccagat ctgtccttcg ttcagttcgc catctccgtg cagggagaag
      601 accagattgc tttccccgtt cattttcttg gtcagggcgg cgccaatggc cacactcatg
      661 ccttgtccga gggaaccaga ggcaatccgt actcccggca gatgctcgtg ggtggcgggg
      721 tgtccctgca gccggctgtt cagtttacgg aaagtagcca gttcttttac atcgaaatag
      781 cccgtccggg ccagaacgga gtaaaaaaga ggcgaaatat gcccatttga cagcacaaat
      841 acatcctcct cccgggcatc catatcaaaa ccgggttttc gcttcattat tctgaaatac
      901 aaagcagtga ggaagtcggc acagcccagg gagccccccg ggtgaccgct gctggctcca
      961 tgtaccatcc ggacgatatc ccttcttacc tgggatgcgg ctgcagttag ttcgttcata
     1021 attaataaaa taggttgtaa ttaaactttt cgtatcttgc tgcctcaaag ggtcagatta
     1081 gttgcaaaag tgcattgttt ttcaatgcaa aaaacagttg taataaattt tatctggcaa
     1141 attcatctaa atatcctctc gattacatag atctgatgtc agataaacta aatgttttat
     1201 gtaatgcaaa aaccattttg gttgatgcct atttatgtac catataaaac tgcttagatg
     1261 cttgatattt tattgacggc ctctgtctct tttataatta cgttcctggc ggtcccggtt
     1321 gttttagaag tggctgaaaa aaagaagttg tttgatatcc ccaatgaacg taaagtccac
     1381 acccgtctgg tggcttcact gggaggtgtt ggtatttttg gtggttttat cctcgcagca
     1441 ctcttatcta ttcagggtca cctgaattat gaatttcaat atttctttgc ggcttccctg
     1501 gttattttct ttctcggatt gaaagatgac ctgattatat tatctgccag taaaaaattt
     1561 atcggacaga tcattgccgc gtccatactg attcacctgg gtggtatccg gctcgatagc
     1621 atgcatggta tcctgggatt tgaaaaatta ccggaagctt tcagcctggc actttcttac
     1681 ctgaccatta ttgtggtcat caattccttt aacctgattg atggagtgga tggactggct
     1741 gccagcctgg gtatcctgac catgctggtc ttcggcagtt atttctttgc gatcggttac
     1801 caggcttatg ccttactggc ttttgcgatg gccggcagcc tggctgcttt tatgatattc
     1861 aatcatcacc cggcccgtat tttcatggga gactccggct cgcttatgat cggactgatc
     1921 aatgcgatac tggtgatcaa atttatcaat gtggccaacg atccgctggt agccgtaccg
     1981 gttccttctg cggtggccat tggttttggt atcctgattg ttcccctgct cgataccctc
     2041 cgtgtattca gcatccggat actgaacggt cagtctccgt ttactcctga ccgcaatcac
     2101 gttcatcacc tgttacttga ccgtggtatg gatcatgcgg cggtaaccct gacctgtgta
     2161 agtgtaaata ttggctttat catccttgcc tggtttggcc gtcacctcgg tccgaattac
     2221 ctgatgttgc tgatgctgac cctggccttt tccggactcg ggctgcttta ctacaaaaga
     2281 ccccgccgtg ctgtgcttat tgccaaaagg agtgatggtg tagccactat gcaaaccaag
     2341 ggtactaaag ttgttaccct gacaaaagaa aaagaagcgc agtccgcagt cgactaatga
     2401 agcgcataaa ataacagccg gccggaccct gaaagtccgg ccggtttttt tggtataagc
     2461 cagttttatt agatttgcgt cccgaagcgt tgaatcttta ttcaaccagg gaaagattaa
     2521 aacgaaaaac tatgtcagaa gaattatcct ccattcattc cggtgcggaa gagcacatga
     2581 aaaaagcaat cagtcacctg gagactgaat tagtaaagat cagggccggt aaagccaatc
     2641 cccagatgct cgatggcatt atggtggatt actacggatc cccgatgccg atcggccagg
     2701 tagccaatgt taatgtaatg gatgcacgta ccctgagtat tcagccctgg gagaaaaata
     2761 tgctgcagcc catcgagcgg gccatcattg ccgccaatat cggtatcaat ccacagaatg
     2821 atggtgtttt aatccgcatg tttctgcccc cgcttacgga ggaaaggcgg aaagaactgg
     2881 taaaaaaatg ccagggcgaa ggcgaacagt caaagattgc gatccgcaat atccggcgcg
     2941 atgccatcga acatatcaaa cggctgcaaa agaacggcct cagtgaggat gtagcgaaag
     3001 atgcggagac cggtgtacag gaaatgaccg ataaatttat atccacagtg gaaaaacacc
     3061 tggcttcaaa agagaaggaa attatgtcga tctgaggaat aactttttta aatgaaaagc
     3121 aacatcattc aaccggtgtt gctttttttg tttccggccg gttggccagg tatacaccca
     3181 gcagaatgat gcctaatgca atcacctgca tgccggttac tttttcgccg taatacacac
     3241 cccatagtat ggcaataaag ggaatgccat acgtgaccag ggaagcaaat aaccctccgg
     3301 cttttttgac caatacataa aataatacgg atgccagggc ggtacccagg atgccgagta
     3361 aagtactggc gattgtactc atggtataga catgctccgt cagcgggagg ttgaaatacc
     3421 ccgtaacgat taaaataatc aaagaagggg gaatcagtcc ggtaaaggcc accgtggcaa
     3481 tctggaccga acttacaccg tgtaatcgtt cgtgcaccat gtttacatta aagccataaa
     3541 aaatagtggc cagcaccaca aacccggtat acgcaaggta cccattgggc ttacccgcat
     3601 ttatatataa aagcaaggca ctgccgacca atccaaccag caccccggaa atttttgtcc
     3661 agctggtttg tttccggaaa aacaatgctc cggtgatgat tacaaaaatc ggggtcagtg
     3721 aattcaggct cccggtaagg gcgccatcta tttttgtctc agcaatacaa aataagaaag
     3781 cagggaaaaa actgcccagc cagccactca gtaatacata tcgcagcgtg ggtgccggta
     3841 aatttttaag tgaacgtttc agaaaaggga gcataaccag accggaggat aacatccgca
     3901 atgccgccac ctggtaggcg gataagaccg gttgctggtt gctgtcatac aatccccatt
     3961 tcatcaggat aaatgaactg ccccagatca cacaaagtgc aatgaagagt gcccagctga
     4021 ttagtttact attcataaag ggggcaaagt tcaggtttca gggggagagt tgggtatttt
     4081 ttttaactta cgttttctta ggaaaagttt aaaagttgac aggttgaaag gtttacaggg
     4141 agcctcctaa gggtcttcct tgttaacttg taaacttttc aacctgtcaa ccttttcgtg
     4201 tatcaaccag tcaacataaa attagaccat gagcaaagta aaactctcct ccctcgatcc
     4261 ccgcgccccc aaagacctcg acaagcaaga cactaaagcc gctactgaaa aaatactggt
     4321 ggaactggat gaattacaaa acctgttata tgctgaaggg aaacattcag tactgattgt
     4381 gatccagggg atggatggca gcgggaagga cggggtgatc aggaatgtac tgggtaatat
     4441 gaatccgcag ggcgtgatgg tgaaatccta taaagcccct acggcagagg aactgtccca
     4501 tgattttctc tggcgcattc atcatcatgc acctgccaaa ggaatgatcc aggtatttaa
     4561 ccggtcacac tatgaagata tcctggtgac cagggtgcat aaatggtgtg atgatgaaac
     4621 ggcccgcaaa cggatggcgg ccatcaatga ttttgaacgg ctgctgcagg aacacaacaa
     4681 tacccatatc cttaaattct acctgcatgt atcacccgaa gaacaacatg agcggctgag
     4741 tgaacggttg aaagagcctg ccaaaatgtg gaaatacaat gagaaggatt ttgatgaagc
     4801 caaactatgg gacatatata tggaaatgta tgaggactgt tttgcccatt gcaataaacc
     4861 cgaatggacg atcgtaccgg ctgatcagaa ctggtataag gagagtatca ttgcaagggc
     4921 tttacgcgat ttgctgaaag gactggatat gcagttccct ggaatgaaaa agtagttttt
     4981 cgtattataa ttatctatat cttgaattga atcctgaaca tttttattaa acccaaaatc
     5041 aattttttat gggaatgctt aaagaattcc gtgattttgc catgaaaggc aatatcgtgg
     5101 atctggccgt agccgtggtt atcggtggtg cctttggaaa aatcgttgac tccatggttg
     5161 gcgatattat catgcctatc ctgggtaaaa tcatgggtga tccgaattat ttcctgacct
     5221 ggacagctgc cggaataccg gttggcaagt ttattcaggc cattttcaat ttcctcatca
     5281 tcgcttttgt gctcttcctg gtgattaaag ccatgaattc tgtgaaaaag aaagaagaag
     5341 ccgccccggc cgcccctgca cctacaccgg aagatatcca gctcctccgc gagatcaggg
     5401 atgccctgaa gaaataaaaa atggggaaaa agggaggttt tcctccgccc cgtttccctt
     5461 acttttgaag tcctgtttaa ccgcaggact tctttttttt cagacctaaa cagcaaccgc
     5521 gtgcagaacc cgtcgtatca gatcaaattg ccccagtttg aaggcccttt cgacctttta
     5581 ttattcttta ttgaaaggga tgaactggat atctataata ttcctattac ccgcattatc
     5641 aacgacttca tggactatat ccaccagagc ggggaactaa atatcgaact cagcagtgag
     5701 tttatcctat tcatttcaac cctgatgcgg atcaaggcca aaatgctcct gccccggaaa
     5761 gaacttgatg cccagggcaa tgaaattgac ccccgggaag aactggtcaa taaaatcctg
     5821 gagtacaaac ggttcaagga agcatccgcg gaaatggccg aaatggaagc cgtacggatg
     5881 ctgatggtga aaagaggaaa tattcaaaag gaactttccc agatcgggga ggaagacagt
     5941 gagggtaccg aaatacagaa tatcaccctc tttaaactga tgaagtcctt tgagaaggcc
     6001 atgcagaagt acagtgaccg cctgaacaag ccggtacaca cggtggtttc atacaactat
     6061 accatggaac aaagccggga cgaaatgctc agcctggccc gcgaagaaaa gaccatcgca
     6121 tttgaacgga tatttgaacg cagtgccaac cgggtacagg ctatcttcct gtttctttcc
     6181 ctgctggaac tcgtacaaca gaagttctta aagattatga tcggggaagg aaagaataat
     6241 tttattattg agtttattga gcccgatgag cgactgaccg aacttgacga acaaaaccaa
     6301 ctataaattt ttaacctcat ttaatttaaa cgcatgaaaa gttttgcaac agccaactgg
     6361 aaaggaaccg gaaaagaagg aaatggcacc ctgtcctcta aatcaaaagt gctcgaaaac
     6421 acgtcttatg acttcagaag ccgctttgaa gacggaacct ttaccaatcc cgaagaactg
     6481 gtagctgccg cccatgccgg ttgtttctcc atgaaactga gctttgtgct gggtgctgcc
     6541 ggctttaccc cggacaatat cgacaccaaa tgcacaatca cccttgaagc aggtgccgta
     6601 accaacagcc acctggaagt gacagcctct gttccgggta ttgatgccgc taaatttgca
     6661 gagtgtgcag cagatgccaa agcaaactgt cctattagta agctgctgaa tacagaaatt
     6721 accatggaag ccaccctggc ctgatgaatc ttgaggtgca ggtccgcagg gggttgatga
     6781 acaggaatta accacgttta aataaaccga catgaaaatt ttatttctcg ccctgttgct
     6841 tacctgcaac cttattttgt acgcccagca aaccagtgcc ggcatgaacg ggacccaacg
     6901 gcggatggaa gtatatagct gggctgaacg tccggaagaa aaggtggaat atgatctcca
     6961 gaaaatgttc ctggaccagc aatggatgcc cggtattgtc agtttcaaaa gtggccgccc
     7021 tgcaatggcc gttccactga tcctggactt gcataacaat accctctatt atcttcaggg
     7081 agccgtgatc atggaatttg ttgataccgt cagccagttc ctcatccggg tgccttctaa
     7141 gaaagacagt atcctgatga agtttaaatg ccagttgcct gccatccaga ccaacaactc
     7201 cgagactttt tatgaagtat tggtagacgg cgatattaaa ttactgaaat gcagggccaa
     7261 gtccatcctg ctttttaaag acaaaaatct gccggaagaa atcaagcagc tctattttgc
     7321 agaactgccc ggtaacaaac tggtgatgat tcccatggat gcagatggaa tcaaagagaa
     7381 attacctgac tataatgccg agatcacggc aatccttaag aaagagcata ttaaactgaa
     7441 gaacgaaaat aaactggctg agttattcgt acacctgaat aacactcagc attagtttta
     7501 ttcatagatt cacaaaagca aaggctgccc ttgtaaaaag gcagcctttg tttcttttgt
     7561 atggtttatt aacctaataa ggtcgacacc gtaatttttt ctttcatcat ttcccggatg
     7621 ctgctggcaa taccttccgc atcataacca cattcacgga tcagttcttt cggggtgcca
     7681 tgctcaacca accggtcggg aatgcccagg atccgcactt cgtttttata accatggcgg
     7741 ttcataaatt ccaggatcgc actgccaaag cccccttcaa cagtaccatc ttccaccgtc
     7801 agcagttttt cataacgctg gcaggcttca tgcagaagtg cttcatccag tggtttggca
     7861 aaacgcatat cgtaatgacc cggattcaat ccatcgtttt tcaggtcacg gatagccgcc
     7921 gcggcaaaat taccgggatg accaaaggaa agaaccgcaa tatcctgtcc gtcccggagt
     7981 ttacgtccct tgccgatttc aatttctttc atcggggtct tccactctgg catcatgcct
     8041 tcgccacggg ggtaacggat caccaccggg agtttagtag attccagttg ggccgtatac
     8101 atcaggttac gcagttcact ttcattcatg ggtgaacaga tcaccaggtt gggaatacaa
     8161 cggaaataag caatatcata acagccgtga tgggtggggc catcatcgcc taccagtccg
     8221 gcgcggtcaa gacaaaagac aaccggtaat ttctggattg ccacatcatg taccacggaa
     8281 tcgaaagccc gctgcatgaa agaagaatag atattacaga agactttgag tccctgggtc
     8341 gccataccgg cactgagggt cacggcatgt tgttcggcaa tacccacatc gaatgcgcgg
     8401 tccggcattt tctccatcat gaatttcagg gaagacccgg aaggcatagc gggggtgata
     8461 ccgaatattt tatcattctg ttccgccagt tcgatcatgg tatgaccaaa cacatcctgg
     8521 tatttgggag gctgtgggat gtcaaatttc tttttctgga tttcaccggt cagtttatca
     8581 aacaaaccag gtgcatgcca cttggtctgg tcctgttcgg ccagcgcata gccttttccc
     8641 ttggtcgtga tcacgtgcag gatcttggga ccgggtattt cacgcagatc gcggagtgta
     8701 tccaccagtt tggccacatt atgaccatca atcgggccaa aatagcgcag gttcagggct
     8761 tcaaagagat tggcactgct gctgaccatc cccttcatcc cctcaatcag tttatgggcc
     8821 atggcccggg aaaagttttt acccacaggc aattttccca tcagtttcca cacatcgtct
     8881 ttcactttat tatacgtggg agaggtggag atatcagtga ggtattcttt cagcgcaccc
     8941 acattcgggt caatgccgat tccgttatca ttcaggatga tcagcatatc agagtccgcg
     9001 acacccgcat ggttcatgcc ttcgaaagcc agtccggcgg tcatggaacc atccccgatc
     9061 acggcgacca ctttacggtc tttttcgcct ttgtatttag ccgccatggc catacccagt
     9121 gctgcggaaa tagacgtgga ggagtgtcct accccgaatg tatcatattc actttcgctt
     9181 cttttgggaa aaccgcttaa tcctttgtat ttacggttgc tggggaaatt gtcccggcgg
     9241 ccggtcagaa tcttatggcc gtaagcctgg tgacctacgt cccagaccag ctggtcatac
     9301 ggcgtatggt acacataatg caaggccgtg gtcagttcca ccacacccag actggcccca
     9361 aaatggccgc catgtacact taccacatca atgatatatt gacgcaactc atcgcagagc
     9421 tggtgaagtt tttcccgggg cagctttttt aagtcatccg gagaatcaat agtttgtaac
     9481 agagggccag gagtgatttg catgggttca catttggtgc tgtaaaagta ctcttttcct
     9541 tcagatcgtc ataatgggcg tttgagggaa aagctgttta acaatatttt gcctgaaagg
     9601 tttacccggt taaacgggtt tttccggtcc acttacaggc attatgcacc actgatatat
     9661 taattcgggt cataaagtga ccgttttctt tagatttgta gcaaatgtgg tttgctaatt
     9721 caaggtactg gttatttcag gcagtgggct ggggcgggtt taccctgatc agtatcttct
     9781 ttgcctattc ttttgatcag ctcaatacaa gggagcagct gacccaggta tttggccgac
     9841 tcgggatatt tgtgatcctg gggatcatgc tcagtcacct gatgcgttcc ctgatcctga
     9901 atgtggacct gctgcagaaa aaactcgaac gccagttttt atatttcctg ctcatcaccc
     9961 ttttgttttc cgtgatcggt tcactgatct atatgcaggt cctgcagcat ttcaacctgc
    10021 tcaacaaacg cgagcatgaa ttcctggaca ataaagtact gctgatagcc agcggcgtct
    10081 ttaacttctt tgtgtatttc tttatctgga accttatcta cttcatttat cattatgtga
    10141 ccaagagccg gaaacagcaa ctggatacct tacggctgga gtcgatggta aaggaactgg
    10201 aactgaaaac catcaaggcc catatcaatc cccattttat ttttaattcc ctcaacagta
    10261 tccgcgccct cgtggatgaa aatccgcaac gggcaagaaa tgcggtgacc gagctcagta
    10321 atattctgcg cagcagtatg cagacggagc aactggagac ggtgccgctg gaacgggaac
    10381 tggggatcat taaggattat ctcgcgctgg agaatatgcg gtttgaagac cggcttaaag
    10441 tggagtatga tattgatgag gatacattga atcagccggt gccgcccatg atgttacaaa
    10501 ccctggtgga gaatgccatc aaacatggaa tcagtaagca gatcaatggc ggattggtaa
    10561 aagtgatttc agcctttaag ggagattatc atgaattggt ggtgcagaat accggtcagc
    10621 tggatggccg gcagccaacg gaagaagggt ttgggttgtc cagtaccact aaccggctca
    10681 gtctgcttta tggtcagaaa gccagttttg aaataaaaca gatcaacgga tccctggtgg
    10741 aagccagggt gcagatcccc gtacaagtat aaaacgattt taacgccacc ccaaaacagt
    10801 atttcatgaa agcaataatc atcgatgacg aacggcttgc gcgtcttgag ttaagaaaaa
    10861 tgcttctgga atttccggaa gtcgaagtaa ttgatgaagc ggtaaatgcg gaggatgcgc
    10921 tggcaaagat cgaagccctg cagccggatc tgattttcct tgatatccag atgccgggca
    10981 aaaccggatt tgatatgctg gccgagctcg acaaggcacc gcaggtgatc ttcaccacgg
    11041 cccaccatga atttgccctg aaggcctttg aggtgaatgc gctggattac ctgatgaaac
    11101 cggtggagcc caagcggctg gccgatgcga tacagaaact gcagcagtcg gagggcaggg
    11161 agcagcgtgg cgaaaccgaa tttgtcaata acagtatcct gtctgagcat gaccaggtat
    11221 ttgttaaaga cggggaacgt tgctggtttg tcaagctcag tgagatccgc ctgtttgaaa
    11281 gtgtgggcaa ttatgccaaa gtttattttg gtgccaacaa gcccctgatc ttaaaatcac
    11341 tcaatgcact ggaagaacgc ctggatgaaa aagttttttt ccgcgccaac cggaagcata
    11401 tcgtcaacct ccgcatgatc gataagatcg agccctattt taacggtgga ttattactcg
    11461 acctgaaagg cggcgagaaa atagaagtga gccggcggca gacggtgaag tttaaggaga
    11521 tgatgagcct ctgagtcagt tcgtagttag tagtcggtag ttagtagtct cgtgttaagg
    11581 gtaaagagtt aaaggtagtt ggaggttgga ggttcgaggt ttcgtgatga gtgtaaagtc
    11641 atttcaatag gggtctttct gctttgaaga aaaggcccga agggctgaca caattataga
    11701 tagtagattt ttggcagcac ccaaagcccc gaaggggtga cacaattgta ggggtgacat
    11761 tattataaac gtcttattca gaacaaaaac caaatgatca gacaatccat tgcaataatc
    11821 ctgttcaccg catcaggatt atccctcgcc gcacagaaag gctttgatat tatcaatgta
    11881 aaagaagtgg aacggatcga aaagaccctc gcctccgatg acatgcgtgg ccgccggacc
    11941 ttcaccccgg atattgacaa agccgctgat tttatcgcgg ctgaattcaa ggccatcggc
    12001 cttggtacaa tgaataatac cggcagctat cgtcaggaat ttgccatggt gcgtcctaaa
    12061 tttatcagcg ccactgctat gctggatggt caggttgttg aacagaaatc ggtgatcgtg
    12121 attacctgtc agccgcaact gaagattgat cagactgctg gttatgaaac cgcttttata
    12181 aaaaccggga ctaacctgca gaccgaagcc cgtaaatatg cccgcgggaa taagaacatg
    12241 atcgtgtggg tggagaaaag ttttgccaat agtttcggca acctggcccg cctgaaatca
    12301 tccatgttta aaacagctac ttccgtggtg tttgtattaa cagataccgc tccggctgcc
    12361 tatacgattg aagcggtgca tgaaatcatc gaacaaaaaa tggccaatgt ggtgggcgta
    12421 atccctggaa aaagcatggt caatgaattt gtggtcttct ctggtcacta tgatcacctg
    12481 ggggtaggta aacctgtgga tggcgattcg atttacaatg gtgccaatga tgatgcggcg
    12541 ggtacaacgg ctgtcatcat gcttgccaaa tattttaaga agctgggtaa caatgaacgc
    12601 accctggtct tcgccgcttt taccgcggag gaagtaggtg gcttcggatc ccagtatttc
    12661 tcccgtcagc tcgacgcgga taaagtaatg gccatgttca atattgaaat gatcggcact
    12721 gaaagcaagt ggggcaaaaa ctccgcctat atcaccggct atgaaaaaac caatatggga
    12781 gatatcctcg ccaaagatct gcagggtact gccttcacat tttatccgga tccctatccc
    12841 acgcaacaat tattctatcg ctccgataat gccactctgg cccgtctcgg agtacccgct
    12901 catacaattt ctacatcgaa aatggatgtg gagccccatt atcacaaact caccgatcat
    12961 attgaaacgc ttgatatggc aaacatgacc gaagtgatca aagccattgc actgagttcc
    13021 aagagtatta tttctgggaa ggagacgcca acgagggtga aggtggagga actcagataa
    13081 aagttcgaag ttggaagttg gaggttggag gtttcgtgat gagggaaaaa acatttcgat
    13141 agggttctgt cttctatcag ataataggcc cgaagggctg acacaattat agaaagaaga
    13201 gttacaataa agcccgaagc cccgaagggg tgacacaatt aacgttggag attcgaagtt
    13261 ggaggtttcg tgatgaggga aaaagcattt cgatagggtt ctttctgcta tcagataata
    13321 ggcccgaagg gttgacagaa ttatagaaag aagagttaca ataaagcccg aagccccgac
    13381 ctgccgacag agacgaagtg aaggagggag ggctgaaatg attatagatt aaagaagcga
    13441 aggatcctgt aatctgaaaa ccctgaaggg gtgacatgat tataccattt tgaacggaat
    13501 agacgaacat aacttagatc gaaactgaat gtccggcctt atcaaccttt taacctttca
    13561 accagtcaac ctgttaccac cggcacactg tgcaccgcat cactcatctc cttaatcaga
    13621 ctggcgtctt tctcaatcgc attaccgact acaatcacat ccgcaccggc cttacagttg
    13681 aggtaggctt tttctggctg gagtatacca ccgcctataa tgagcggcac ttctatatga
    13741 tcggccacgg ccttgatcat actttcggta atgggacgtt tggcaccgct gccggcatcc
    13801 atataaataa gcttcatgcc cagcatttcg ccggccatgg ctgtacacat cgcaatttca
    13861 tttttgtcag caggtacagg tgcggcatta ctgatatagg ataccgtagt gggggctccg
    13921 ccatccacca caatataacc ggtcggcata atctccagtc cgctcttttt cacaaaagga
    13981 gcggaaacca catgctgacc aatcagcaat tcaggattcc ggccggagat cagggataag
    14041 tataataagg catcagcata cttactcacc tgggagggac taccaggaaa caggattacc
    14101 gggatatcac agcttttttt gatctgttgt acacattcat ccaggtgatt ggagatca
//
LOCUS       DAOPMW010000044        14012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4409_length_14012_cov_5.288386, whole genome shotgun sequence.
ACCESSION   DAOPMW010000044 DAOPMW010000000
VERSION     DAOPMW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4409_length_14012_cov_5.288386"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2093
                     /gene="secD"
                     /locus_tag="PLU11_03980"
     CDS             <1..2093
                     /gene="secD"
                     /locus_tag="PLU11_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPN58303.1"
                     /translation="NVINKFIQFDLPRQDEKGIMRFPAWIGYVMIRDTAAFRNYLNEF
                     KSNFPSDAVFAYGIPDKDKKGVALEFVPLYALKTFNRTSAKLEGDAISDARQDFDQYG
                     KVAVQMNMKPGGAAIWKKMTEDNVGKPLAIVLDNVVQSAPNVNNVIPSGESSITGSYT
                     QQEAADLANILKIGKLPAPAKIVHEEQVGPTLGAKAIKGGTNAFLISFVVIFLLMLVY
                     YNTSGWVANIALILNLLFTVGILAGLGATLTAPGIAGLVLTIGMAVDTNVIIYERIKE
                     ELSKGKGYIPAINEGYKRSLPPVLDAHVTTLLTAFILFYFGLGPVKGFATTQIIGILL
                     SLFCGILVSRWVSDIFTNKKKHLEYFTGVSRRVFKHAKYKFIEYRKIAYMISVVVLIL
                     GIASYFNGFKYGVEFDGGRSYTVQFTQKVNEEAVRQALEKSFEKENTIIKTIGDNSKL
                     DITTSYLIKNTSLSADSVVSQKLYEGLKSFLPADLTYEQFATEKYIQQSKKVLPTISD
                     DLKAGAVKATIFAMLVIFLYIFIRFRDWRYSLGTIVALLHDVFVTLIVFSFAKNLVPF
                     PLEIDQHFIAAVLTVIGFSMNDTVIVFDRIREDSRMMPTAPRGEVINRAINETLSRTI
                     MTSLTVFLTILILFLVGGEVTKGFAFAMLIGVITGTYSSIFVAAPILVDLGGKRPLGK
                     AAEKPKPGAAEKKA"
     gene            complement(2449..2793)
                     /locus_tag="PLU11_03985"
     CDS             complement(2449..2793)
                     /locus_tag="PLU11_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPN58304.1"
                     /translation="METAVTIPVTLTEGAIAEVRRLMAADGFDHSQKLRIGVKGGGCS
                     GMTYVLGFDQPGANDQHFEIAGIPCVMDKSHELYLHGMLVDWQDGLNNRGFTFKNPNA
                     STTCGCGTSFAV"
     gene            2946..3452
                     /locus_tag="PLU11_03990"
     CDS             2946..3452
                     /locus_tag="PLU11_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58305.1"
                     /translation="MDLTLVSLIISVLAILLSFFAVSRTVSGKQETPVTNNSFESVPL
                     RLQAYERLVLLTERISLTSLISRLNQPGISAAEMKVILTENIKQEYDYNSTQQLYVSQ
                     VSWDAVRNLKEQNIMVINQVAASLPHTASSSELNKKILEVMMSQQNGALHEMVSQTLN
                     HEAKKLMQ"
     gene            3482..5041
                     /locus_tag="PLU11_03995"
     CDS             3482..5041
                     /locus_tag="PLU11_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPN58306.1"
                     /translation="MDPQKKYTDSGIEVKQLYTGEDLPVLNNEMPGQFPFTRGVQPDM
                     YRGKLWTMRQYAGFSTAEESNKRYHYLLSQGVSGLSVAFDLPTQIGYDSDHALAEGEV
                     GKVGVAIDSIEDMQILFNGIKLEEVSTSMTINATGYILLSLYVALAKQQGADLKKISG
                     TIQNDILKEYAARGTYIYPPKPSMRIITDIFEWCSAEVPKWNTISISGYHIREAGSTA
                     VQEIAFTLANGKAYVQAAQQKGLDINVFGKRLSFFFNAHNNLFEEVAKFRAARRMWAK
                     IMKDLGATDPKAMMLRFHTQTGGSTLTAQQPLNNISRVTIQTLAAVLGGTQSLHTNGY
                     DEALSLPTEAAARIALRTQQIVAFESGAVDTVDPLAGSYYVESLTQEVETAAWKLIEK
                     IDAMGGSVSAIEEGFIQDEIARSAYEYQRNIENNEKIIVGVNKFQSEEQNNTPILKVD
                     DSIRIVQAEKLRQLRSNRDHAKCDQLLQLLSDKANSGENIMPVVVEAVEAKCTLGEIA
                     DTLRELYGEYK"
     gene            complement(5210..5590)
                     /locus_tag="PLU11_04000"
     CDS             complement(5210..5590)
                     /locus_tag="PLU11_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58307.1"
                     /translation="MSYTTITRIGTDHTIWQKGFGFYKDELDIMESRLTEIAKRNTTF
                     EARQGVEHFQNQFIVQRNNIDELKHEVNIYVGKLGADAQQHGGRMETHMIEEHAALHG
                     KYEDFERVMNILRHEFNEYLSKWM"
     gene            complement(5801..7462)
                     /locus_tag="PLU11_04005"
     CDS             complement(5801..7462)
                     /locus_tag="PLU11_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPN58308.1"
                     /translation="MSEERSLNFLEEIIEEDLKNGKYKSIVTRFPPEPNGYLHIGHAS
                     SICLNFGLTQKYPGFTNLRFDDTNPVTEETEYVESIKEDVKWLGFEWKNERYASDYFD
                     TLYAYALKLIDKNLAYVDDISAEEMAAMKGTPTEPGKDSPYRNRSVEENRDLFVRMKN
                     GEFPDGVRTLRAKIDMGHINMLMRDPIIYRIKHAHHHRTGDKWCIYPMYDFAHGQSDS
                     IEEITHSICTLEFVPHRELYDWLIEKLEIYPSHQYEFARRNINYTVTSKRKLLQLVNE
                     KHVTGWDDPRMPTISGLRRRGYTPESVREFCDRIGVAKRDNMVDVGVLEFCVREHLNK
                     IALRRMVVFDPLKIVITNYTMDEEILLSENNPEDEQGGSREIPFSNEIYIERDDFMEV
                     PPKKYFRLAPGQMVRLKSAYIIKCEEVLKDEAGHITEIRCTYIPESKSGEDSSGIHVK
                     GTLHWVSVRHAAKATVRLYDRLFRVEDPTDEAGDFKEYLNPDSLQELPVVYAEPSLKQ
                     ASLQERYQFIRKGYFVLDRDSANGNLVFNKTVGLKDAWAKEVKKG"
     gene            7621..8466
                     /locus_tag="PLU11_04010"
     CDS             7621..8466
                     /locus_tag="PLU11_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN58309.1"
                     /translation="MNRILVPTDFSPNADRAIDYAVRIARLNESTLYLIHACDNLDPL
                     YMEGAMTHMEYNQKIMDEAFEKLDIRRKSIEDTEGVLVNIQLYSGSVMDTIMVAALEH
                     KADLIIMGTLGISGLRDRIFGSKTASLISESNIPVLAIPLEYDWSQPGKFLIAIKDVD
                     EALPLLDPAFELASLFNAEVHITLFTDKDDGGALDYISDDRIITRAETKLKGQHKQVL
                     IKKEHLLGGNFEDSINEYIAQNGIDLLAMITHKRSLLGRLFHQSITRKMSYHTRVPVL
                     AIPAG"
     gene            8488..9342
                     /locus_tag="PLU11_04015"
     CDS             8488..9342
                     /locus_tag="PLU11_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN58310.1"
                     /translation="MKTIVVPFDFSANARKALSTAFQLAKATASGILVVHVVHQSPYK
                     LAAAGNENEMNRLIRQDEMEKKIALQQETAEIQQALSLKFPAGQIMVKAVYHPLVVEK
                     IIELAHAEDAGLIVMGTHGASGLRKILFGSNAANMISRSDIPVLAIPQDYEYKTVEKI
                     LYASDLENLEAELKRIIPFAIALNAVIEVLHLDYGENDNKISLEEAEKLIHKSPYQFI
                     NLLIREANLNKPLLAQLKAHTELVKPEWLIMFTQVKSMWDKLFKGSHTEDMSQALPLP
                     MMSFKKPE"
     gene            complement(9344..9847)
                     /locus_tag="PLU11_04020"
     CDS             complement(9344..9847)
                     /locus_tag="PLU11_04020"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN58311.1"
                     /translation="MQERVQQLIQHYQMQPHPEGGFFLQTYKSSGSIPQSVLPEGFAG
                     PRPFSTAIYFLLEQGNFSAFHRIRSDECWHFYEGQTLLVHMLNPDGSLTTIRLGPDIA
                     HGELYQYVVPANCWFASEPAPGTSYSFVGCTVSPGFDFADFELADTTTLMTGFPGQRS
                     IIQRLCR"
     gene            complement(9847..11655)
                     /locus_tag="PLU11_04025"
     CDS             complement(9847..11655)
                     /locus_tag="PLU11_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPN58312.1"
                     /translation="MKDNYDKFEYRIPMRDGAKLFTLVYMPKDKSKTYPILMNRTCYN
                     ASNYGGFNTHGHPSSFLVHDGYILVFQDVRGRYMSDGLFNNMTPNIPGNDPKNKKDID
                     ESSDTWDSIDWMVKNLAGNNGKAGIYGISYPGFYSAASLPDAHPALKAVSPQAPISDF
                     FFDDFHHMGAYLQSYTAAFAVFGYQKKDTTRKDWFNEEMMRVYSKPVKDGYDFHLKMG
                     PLKNVTEKLHYDNFFWNQIVDHPNYDTFWQKRSILPHLKNTKPAVLTVGGWFDAEDLY
                     GPLNIYKTIEKNTPGAKNSIVMGPWGHGDWARENGKSTHNHIYFGDSISTFFQKEIER
                     NFFNFHLKGEGTMALPEAYMFNTGKKEWQKFDVWPPTEIPPVKLTFGENGKLFINQPA
                     NEQLRFEYISDPAKPVPYTSQTEGLTFTPRNYMSDDQREAARRPDVLTFETEVLGDEV
                     TLAGEILAKLKVALSTTDADFVVKLIDVYPDNHTNYPHNPKNVIMGGYQQLVRSEVFR
                     GRFRNSFEKPEPFVPGKVTEVNVPLQDVLHTFKKGHRIMIQIHSTWFPFIDRNPQTYV
                     ENIYKADEKDFIKSTIKVYGSSVVGAGGEQTPKKAF"
     gene            11850..12383
                     /locus_tag="PLU11_04030"
     CDS             11850..12383
                     /locus_tag="PLU11_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58313.1"
                     /translation="MRKFLVLLLTFFIHGTIFSQEWLTFEDSSSTFTAKYPATWTKKI
                     RADGIVVFSSPISGDTDNFKENINVIVRKSALFTKDFDLKEALPSIIDKLNSSLTDFK
                     QNSSRFFKWNGLDAAEIKFTVTNKAEDQDNPLPLKMMQWYCSGEGVFYTATFTAHRDN
                     NQFDAEALELFQSIRFR"
     gene            12441..12590
                     /locus_tag="PLU11_04035"
     CDS             12441..12590
                     /locus_tag="PLU11_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58314.1"
                     /translation="MSLLNQGNKKDKKKEDKKKGAAGSGSKFMAKPKSGFVSKQQNTG
                     SQRGS"
     gene            12606..13022
                     /locus_tag="PLU11_04040"
     CDS             12606..13022
                     /locus_tag="PLU11_04040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HPN58315.1"
                     /translation="MNREELLKELSEETFITLKPSPVHGIGVFALRDIPRGCKTLFSR
                     NVGEWIRVPISDIDQLPDQTRNMVETYCLYDETHYYLPDYGFKVMDLVNYLNHSSTPN
                     VISVNDGEYFESLREIKEGEELLVNYGEIVDTKEYL"
     gene            complement(13212..13691)
                     /locus_tag="PLU11_04045"
     CDS             complement(13212..13691)
                     /locus_tag="PLU11_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58316.1"
                     /translation="MRQALISICLLFSSAVYAQSDSDTTLAHCPVFITDTVTANNFFI
                     EARPVLMKVYRVKGKLTIRLEQKDQFFTLYFHEKKLRNTTYKIQAGSTGKSEVEAAYS
                     FRTGDQSSYISVAKGSIDCYFDTEKQSWQLNISGLIINQVERAVTHYRVKGKLVVGS"
     gene            complement(13747..>14012)
                     /locus_tag="PLU11_04050"
     CDS             complement(13747..>14012)
                     /locus_tag="PLU11_04050"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPN58317.1"
                     /translation="SKDQPLKNIRIRGFMGMASFSDNPVQVRAEFRQLKNFYDQYKSD
                     SSPAFRIDTLSMGMSGDYKIAMEEGGNMVRIGSLLFGERNRPV"
BASE COUNT         3889 a         3333 c         3143 g         3647 t
ORIGIN      
        1 tcaatgtaat caataagttt atccagtttg acctgccccg ccaggatgag aaaggtatta
       61 tgcggtttcc ggcctggatc ggttatgtaa tgatccgtga tacggctgct ttccggaatt
      121 acttaaatga atttaaaagc aatttcccca gcgatgcggt gtttgcttat ggtatacctg
      181 ataaagataa aaaaggagtg gccctggaat tcgtaccact ctatgcgctt aaaacattta
      241 accgtacttc agccaaactt gaaggggatg ccatttcgga tgcccgccag gattttgacc
      301 agtatggtaa agtagcggtg cagatgaata tgaaacccgg tggtgctgct atttggaaaa
      361 aaatgaccga agacaatgtc ggtaagccac tcgcaattgt gctggataat gtagtacagt
      421 ctgccccgaa tgtaaataat gtgatcccca gcggcgaatc atccatcacg ggtagttata
      481 cccaacagga agcggccgat ctggcgaata tcctgaaaat tggtaaactc ccggctcctg
      541 ccaagattgt acatgaagag caggttggac cgaccctcgg tgccaaagcc atcaaaggcg
      601 gaaccaatgc attcctgatc tcttttgtgg tgatcttcct cctgatgctg gtctattata
      661 ataccagtgg atgggtggcc aatatcgccc tgatcctgaa cctgctcttt accgtaggta
      721 tcctggccgg actgggtgct acgttaaccg cccctggtat tgccggtctg gtactgacca
      781 ttggtatggc ggtggatact aacgtaatta tctatgagcg tattaaagaa gaattatcca
      841 aaggcaaggg ctatatcccg gcgattaatg aaggatataa acgttcattg ccacctgttc
      901 tggatgcgca cgtaactacc ttattaaccg catttattct cttttacttc ggactgggtc
      961 cggtaaaagg gtttgccacc acacagatca ttggtatcct cctttccctg ttctgcggaa
     1021 tcctggtgag ccgttgggtg agtgatattt ttaccaataa gaaaaaacac cttgaatact
     1081 ttaccggtgt ttcccgccgc gtattcaaac atgcgaaata caagtttatt gaataccgga
     1141 agatcgctta tatgatttcc gtagtggtac tcatcctggg tatcgcctcc tatttcaatg
     1201 gatttaaata tggagtggag tttgacggcg gacgtagcta taccgttcag ttcactcaga
     1261 aagtaaatga ggaagccgtg cgacaggccc tggaaaaatc cttcgaaaag gaaaacacga
     1321 tcatcaagac cattggtgat aattccaaac tggatatcac cacatcatac ctgatcaaga
     1381 ataccagtct gtctgctgac tcagtagtat ctcagaaatt atacgaaggc ctgaaaagct
     1441 tcctgcctgc tgacctgaca tacgaacagt ttgcgactga gaaatatatc cagcagagca
     1501 agaaggtact gccgaccatt tcagatgacc tgaaagccgg tgcggtgaaa gccaccattt
     1561 ttgcgatgct ggtaatcttc ctgtatatct tcatccgttt ccgcgactgg aggtattcac
     1621 tgggtacgat tgtagccctg ttgcacgacg tatttgtgac attgattgta ttctcttttg
     1681 caaagaatct cgtgcctttc ccgctggaga tcgaccaaca ttttatcgca gcggtgctga
     1741 cggttatcgg tttctccatg aacgatacgg tgatcgtatt tgaccgtatt cgtgaagaca
     1801 gccgtatgat gccaactgca ccaagaggtg aggtgatcaa ccgcgcgatt aacgagaccc
     1861 tgagccgtac gatcatgact tccttaacgg tattcctgac catcctgatc ctgttcctgg
     1921 taggtggtga ggtaaccaaa ggtttcgcct tcgcgatgct gatcggtgta atcaccggta
     1981 cctactcttc tatcttcgtg gctgccccga tcctggtaga cctcggtggt aaaagacccc
     2041 tcggtaaggc cgctgaaaag ccgaagccgg gtgctgctga gaaaaaagcg taagtaaccg
     2101 ctaaaaatat aaagaggccg tcttaacaga cggcctcttt tttttgcctg cggcaaaaaa
     2161 gggttggcca ctaaatcacg cgaaagggca cgaagaatac aaatacagaa aggcaggcca
     2221 cggatcacac agatcagcac ggattctatt tcgtgagact gcaaaaaaaa tatatcccgc
     2281 aaagcttttt aagacattcc gataagggta gcatttttaa tgtacggaca gtctttaatt
     2341 cttaacagga tgcaaatctt ctatagttgg aaaattaatc cgtgctgatc tgtgtgatcc
     2401 gtggcctgta tttcttcgtg ccctttcgcg tgatttagtg gccaaatatt aaacggcgaa
     2461 agaagtaccg catccgcagg tcgtgctggc gttgggattt ttgaaggtga agccgcggtt
     2521 attgagtccg tcctgccagt ctacgagcat cccgtgcagg tagagctcat gtgatttatc
     2581 catcacacag ggaataccgg ctatttcaaa atgctgatca ttggctcccg gctggtcaaa
     2641 accgagtaca taggtcatgc cggagcatcc tccgcccttt acccctattc tcagtttctg
     2701 gctatgatca aaaccatcag cggccatcag gcgtcttact tctgcaattg ctccttcggt
     2761 gagcgttacg ggtatcgtta cagctgtttc catccctcaa aattaatcaa agttttgact
     2821 gttaaaagcc cctgcatttc cgaagaaggg aatttaactg acaattatca gtccgctgac
     2881 tgccagtacg gtaccggaaa gatggtccaa tcagcctatt tttgcaacaa atcaaaatca
     2941 tctctatgga tcttaccctt gtaagtctta ttatttctgt gctggccatt ctgctatcct
     3001 tctttgccgt atcccggact gtatcgggca aacaggaaac tccagttacc aacaactcgt
     3061 ttgaaagtgt cccactccgt cttcaggcct atgaacgcct ggtactcctg accgaacgta
     3121 tttccctgac cagcctcatc agccggttga atcaacccgg aatttccgcg gctgaaatga
     3181 aagtgatcct gaccgagaat atcaagcagg aatatgatta taacagcacc cagcaattat
     3241 atgtgagcca ggtcagctgg gatgcggttc gcaacctgaa agaacaaaat ataatggtca
     3301 tcaaccaggt cgccgccagc ctgccccaca cggcttcttc atcagagctc aataaaaaaa
     3361 tactggaagt catgatgtca cagcaaaacg gtgccctgca cgaaatggta tcgcaaaccc
     3421 tgaatcatga agccaagaaa ctgatgcaat aacgattttt cccacccatc aattttcaat
     3481 tatggacccg caaaaaaagt atactgattc cggaatagaa gtcaaacaac tctataccgg
     3541 ggaagacctt cctgtgctca acaatgagat gcccggccag ttccctttta cccggggtgt
     3601 tcagcccgat atgtaccgtg gcaaattatg gacgatgcga cagtatgccg ggttctccac
     3661 cgccgaagaa agtaataaac gctatcacta tctgttgtca caaggggtta gcggactgag
     3721 tgtggctttt gacctgccta cacagatcgg gtacgacagt gaccatgccc tggccgaagg
     3781 agaagtcgga aaagtgggcg tagccatcga ttctattgag gatatgcaaa tccttttcaa
     3841 tggaataaaa ctggaagagg tcagcacatc catgactatt aacgccactg gttatatcct
     3901 tctctcatta tatgtggcac tggccaagca gcagggcgct gacttgaaga agatttccgg
     3961 aaccattcaa aatgatatat taaaggaata cgcggcccgt ggtacctata tctatccgcc
     4021 caaaccttcg atgcgcatca tcacggatat ctttgagtgg tgcagtgccg aagtgcccaa
     4081 gtggaatacc atctccatat ccggttatca tatccgggaa gccggcagta ccgctgtaca
     4141 ggaaatcgct tttaccctgg ccaatgggaa ggcctatgta caggccgcac agcaaaaagg
     4201 actggatata aatgtctttg gcaaacgcct ctccttcttt tttaatgcgc ataataatct
     4261 gtttgaagaa gtggcgaaat tcagggcggc acgcaggatg tgggcgaaga ttatgaagga
     4321 ccttggcgcc actgatccca aggctatgat gcttcgtttt catacccaga ccgggggaag
     4381 cacgcttacc gcccagcaac cattaaacaa tatatccaga gttacgatcc agaccctggc
     4441 cgctgtattg ggcggcacac agtcgctgca taccaatggc tatgatgaag ccctgagtct
     4501 gcccacagaa gccgccgccc ggattgccct acgtacccag cagatcgtgg cttttgaaag
     4561 cggggcggtg gataccgttg acccgctggc tggttcttat tatgtggaat ccctgacaca
     4621 ggaagtggaa acagccgcct ggaaactgat tgaaaaaatt gatgccatgg gcggcagtgt
     4681 gtcggccatc gaagaaggat ttatccagga cgagatagcc cggagtgctt acgagtacca
     4741 gcgcaatatt gagaacaatg agaagatcat tgtgggagtg aataaattcc agtccgaaga
     4801 acaaaacaac accccgatcc tgaaagtaga cgacagtatc cggatcgtac aggcagagaa
     4861 attaagacaa ctgcgctcca accgggacca tgccaaatgt gatcagctgc tccagttact
     4921 cagtgacaag gccaacagtg gcgaaaatat tatgcccgtg gtagtagaag ccgtggaggc
     4981 gaagtgtaca cttggggaaa ttgcagatac gttacgcgaa ctgtacggcg aatataagta
     5041 aggttgacaa gttaataggt tgaaaagttg acaggttgaa aagttgacaa ggaagttttc
     5101 ttagaagcgg ccttgttaac ctgtcaacct gttaacctgt caactatacc taaaacaata
     5161 agggccactt cctttagaaa atggccctta aataacaatt actaacccct cacatccact
     5221 tactcaggta ttcattgaat tcgtggcgaa ggatattcat tacgcgttca aaatcttcat
     5281 atttcccgtg aagggcggca tgctcctcaa tcatgtgggt ttccatacgg ccaccatgct
     5341 gctgcgcatc ggcacccagt ttgcccacat agatattcac ttcatgtttc agctcatcaa
     5401 tattgttgcg ctgaacaatg aactgattct ggaaatgttc cacaccctga cgggcttcga
     5461 aagtggtatt ccttttagcg atctcggtca ggcggctttc catgatatcc agctcgtctt
     5521 tatagaatcc aaaccctttt tgccagatgg tatggtcagt cccgatccgg gtgatggttg
     5581 tataactcat agcggattgt tttaaccaaa gctatctttc caatttcctt attttgctga
     5641 ctcctgtcag cgaaggatat gatagaattc atcggcctta aagactttta ttagccaatt
     5701 ccttcattta caccgccgaa gccagcaaag agcgtcaaaa taaaaaaagc gccgtaaagg
     5761 gcgcttctgt tcgttagatg acgttcctgc tcccgggtga tcaacctttt ttcacttctt
     5821 tagcccaggc atcttttaat ccgaccgtct tattaaaaac caggttgcca ttggcagaat
     5881 cgcggtccag tacaaaataa cccttgcgta taaactggta acgttcctgt aaactggctt
     5941 gtttcaggga gggttcagca taaactaccg gaagctcctg taatgaatcc ggattcaggt
     6001 attccttaaa atcaccggct tcatccgtcg ggtcttctac ccggaagagg cggtcgtata
     6061 aacggaccgt ggcttttgcc gcgtgccgga cactcaccca atgcaacgtg ccttttacat
     6121 ggataccgct gctgtcctcc ccactcttgc tttccggaat ataagtacac cggatttctg
     6181 taatgtggcc ggcctcatct tttaagactt cctcacattt aataatatag gcgcttttca
     6241 gtcgtaccat ctggccaggt gccagccgga aatatttttt cgggggtact tccataaaat
     6301 catcccgttc gatatatatc tcattgctga atggaatttc cctggaacca ccctgttcgt
     6361 cttccggatt attctcactc agaaggatct cttcatccat tgtataattc gtgataacaa
     6421 ttttcaacgg gtcaaatacc accatccgtc gcagggcaat tttattcagg tgttccctga
     6481 cacaaaactc gagtacaccc acatccacca tattatccct tttcgccacc ccgatacgat
     6541 cacagaattc acgaacactt tccggggtat accctcttct ccgcaatcca ctgatcgtgg
     6601 gcatacgggg atcatcccag ccggtgacat gtttctcatt taccagctga agcagtttcc
     6661 ttttactggt tacggtataa ttgatattac gacgggcaaa ctcatactga tgtgaaggat
     6721 agatttccag tttttcgatc agccagtcat atagttcccg gtgcggaaca aactcgagcg
     6781 tacaaatgga atgggtgatt tcttcaatgg aatcactttg gccatgggca aaatcataca
     6841 tcgggtaaat acaccattta tccccggtac ggtgatgatg cgcatgcttg atccggtaaa
     6901 tgatcgggtc ccgcatcagc atattgatat ggcccatatc aatttttgcc cggagcgtac
     6961 gaaccccgtc gggaaactcg ccatttttca tccggacaaa taagtcacgg ttttcttcca
     7021 cagaccggtt ccggtaagga ctgtctttac ccggttcagt cggcgttcct ttcatggccg
     7081 ccatttcctc cgccgaaata tcatccacat aggccaggtt cttatcaatc aacttcaggg
     7141 cataagcata cagggtatca aaatagtcgg aagcatagcg ttcatttttc cactcaaatc
     7201 caagccactt cacatcctcc ttgatacttt ccacatactc ggtttcttcc gttaccggat
     7261 tggtatcatc aaatcgcagg tttgtaaagc ccgggtattt ctgggtaagc ccgaaattca
     7321 ggcagatgct ggaagcatgc ccgatatgga ggtacccgtt cggctccggt gggaaacggg
     7381 tcacaatcga tttgtattta ccgtttttca ggtcctcttc gatgatttct tccaaaaaat
     7441 tcaaactacg ttcttcagac ataactcttt tataataaag ggcaaagata cgaaatatca
     7501 gttagcagcc accgtagttc cccatgcccg ccggctcaat taaaatcagc ataactgttg
     7561 agagcgatca gtaaaataaa ggttatattg gaggaacttg ctaacagtta aacccataca
     7621 atgaacagga tactggtacc taccgacttt tcacccaatg ccgaccgggc gatcgattat
     7681 gccgtccgga ttgcgcggct aaacgagtcc acgctgtacc tgatccatgc ctgcgacaac
     7741 ctggaccccc tctacatgga aggggccatg acgcatatgg aatacaatca aaagatcatg
     7801 gatgaggctt ttgagaaact ggacatccgg cggaagagta ttgaagacac agaaggagta
     7861 cttgtaaata tccagttgta cagtggcagc gtgatggata ccattatggt ggccgccctg
     7921 gaacataagg cggacctgat aatcatgggt acactgggta tctccggatt acgggaccgg
     7981 atatttggca gtaaaacggc ctccctgatc agtgaaagca atatccccgt actggcgatc
     8041 ccccttgaat atgattggag ccagcccgga aaattcctga ttgccatcaa agatgtggat
     8101 gaagcccttc ccctcctcga cccggctttt gaactggcct ctttatttaa tgcagaagtc
     8161 catatcaccc tgtttactga taaggatgat ggaggcgcac tcgattatat ttcagatgac
     8221 cggattatca cccgggcgga aacaaaactg aaaggacaac ataaacaggt cctcatcaaa
     8281 aaagaacatt tgctgggtgg aaatttcgaa gacagtatca atgaatatat tgcccaaaac
     8341 ggcattgacc tgctggccat gatcacccat aagcgaagcc tgcttggccg gcttttccat
     8401 cagagcatca cccgtaaaat gagttatcat acccgggtac cagtcctggc gattccagcc
     8461 ggctaatcac tcaaaaattg aaagcatatg aaaacgatcg tggtgccttt cgacttctcg
     8521 gccaatgccc gtaaagcatt atccactgct tttcaactgg ccaaagcaac cgcttccgga
     8581 attctggtgg tgcatgtagt gcatcaatcg ccttacaaac tggcagcagc gggcaacgag
     8641 aacgaaatga accgccttat ccggcaggat gaaatggaaa agaaaattgc cctgcaacag
     8701 gaaacagcag aaatacaaca agccctttcg ctaaaattcc cggccggaca aataatggta
     8761 aaagcggttt atcatcccct ggtcgtggaa aaaattattg aactcgcgca tgcagaagat
     8821 gccggcctga ttgtcatggg aactcatggt gccagcggtt tacgaaaaat ccttttcgga
     8881 tcaaatgcgg cgaacatgat ttccagatcc gacatacctg tgctggccat accccaggac
     8941 tatgaatata aaacggtgga aaaaatatta tatgccagtg acctggagaa tcttgaagct
     9001 gaactaaaac ggatcatccc cttcgcgatt gccctgaatg cagtaataga agtgctccac
     9061 ctggactatg gtgagaatga taataaaatc agtttggagg aagcggaaaa attgatccat
     9121 aaatcgccct accagtttat caacctgctg atccgtgaag caaatctcaa taaaccatta
     9181 cttgcacaac tgaaggccca tactgaatta gtgaagcctg aatggctgat catgtttacc
     9241 caggtaaaaa gtatgtggga taaattattt aagggcagcc acactgaaga tatgtctcag
     9301 gcgcttccgt tacccatgat gagttttaaa aaaccagaat aattcagcgg cacaaccgtt
     9361 gaataatgct gcgttgtccc ggaaaaccgg tcataagcgt ggtcgtatcc gccaattcaa
     9421 aatcggcaaa atcaaacccg ggtgacacgg tgcatccaac aaaagagtaa gaagtgccgg
     9481 gagctggttc actggcaaac cagcagttgg ctggaaccac atactggtaa agttccccat
     9541 gagcaatatc cgggcccagc cggatcgtgg ttaatgatcc atccgggtta agcatatgca
     9601 ccaacagggt ttgtccctca taaaaatgcc agcactcatc acttctgatg cggtgaaaag
     9661 cggaaaaatt accctgttcc agtaaaaaat agatcgcggt ggaaaaagga cgcggaccgg
     9721 caaaaccttc cggaagcacc gactgcggta tactcccgga agatttatag gtctgcaaaa
     9781 aaaatcctcc ctccggatgc ggctgcatct gatagtgttg aatcagttgt tgtacacgtt
     9841 cctgcatcag aatgcttttt taggggtctg ttcaccccct gctccgacta cagaactgcc
     9901 atacactttt atagtggatt taataaaatc cttttcatct gccttgtata tattctctac
     9961 ataggtctgc gggttgcggt caataaaggg aaaccaggta ctgtgaatct ggatcatgat
    10021 ccggtgtcct tttttaaatg tatgcaacac atcctgcaaa ggcacattca cctccgtcac
    10081 ctttcccggt acaaagggtt ccggtttttc aaaactgtta cggaaccttc cacggaatac
    10141 ttcactacgc accagttgct ggtagccgcc catgattacg tttttgggat tatgggggta
    10201 gttggtatga ttatcgggat acacatcaat cagtttgacc acaaaatcgg catcggtggt
    10261 ggaaagcgcc actttgagtt tggcgagtat ttccccggcc agggttactt catctcccag
    10321 tacctctgtt tcaaaagtca gtacatccgg acgacgggcg gcttcccgct ggtcatcact
    10381 catatagttg cggggggtaa acgtcagtcc ctctgtttgt gaggtatagg gtaccggttt
    10441 ggccgggtca ctgatatatt caaaacgcag ttgttcgttg gccggctgat taatgaacag
    10501 ttttccgttt tcgccaaacg tcagctttac aggtgggatt tcggtgggag gccatacatc
    10561 aaatttctgc cattcctttt tcccggtatt gaacatatag gcttcgggaa gggccatcgt
    10621 tccttcgccc ttcaggtgga aattaaaaaa attacgttct atttctttct ggaaaaaagt
    10681 ggaaatgctg tcgccaaaat aaatatgatt atgtgtgctc ttcccatttt cacgggccca
    10741 gtcaccatgt ccccaggggc ccatgacgat actgtttttt gcaccgggcg tgttcttttc
    10801 aatcgtcttg taaatattca acggaccata caggtcttct gcatcaaacc atccacccac
    10861 ggtgagtacc gccggctttg tattcttcag gtgaggaagg atacttcttt tttgccagaa
    10921 tgtatcatag ttcggatgat ccacaatctg gttccagaaa aaattatcat agtggagttt
    10981 ttcggtaaca tttttcagcg gccccatttt caggtgaaaa tcgtacccgt ctttcaccgg
    11041 cttgctgtaa acacgcatca tctcctcatt gaaccaatcc ttacgggtgg tatctttttt
    11101 ctggtaaccg aaaacggcaa aggcggcagt ataactttgc aggtaagccc ccatgtgatg
    11161 aaaatcatca aaaaagaaat cggaaatggg tgcctgcggg gacaccgctt tcagggccgg
    11221 gtgagcatcg ggtaaggagg cggctgaata aaagccagga taagatatac catagatacc
    11281 tgctttacca ttattgccgg ccaggttttt caccatccag tcaatactgt cccaggtatc
    11341 cgaactttca tcgatatcct ttttgttctt cggatcattg ccggggatat tcggggtcat
    11401 attattgaat aagccatcac tcatatagcg gccccggaca tcctggaaga ccaggatgta
    11461 tccgtcatgc accaggaagg aggaaggatg tccgtgtgtg ttgaaacctc catagttgga
    11521 ggcattgtaa caggtccggt tcatcaggat cggataggtc tttgacttat ccttgggcat
    11581 atacaccagg gtgaacaact tagccccgtc ccgcatcggg atacggtact caaacttatc
    11641 atagttatcc ttcatatact gtgtttcggg atccgcagac tggccaataa gcaatccagg
    11701 aacgccgaaa agcaataaaa aaatgaaaaa tctatacata attttttagg ggaagataaa
    11761 gcaagaacgg gtaaattttc aaggttatta tacacgggac tttcacaagc cgataattaa
    11821 ctatattttc ggggataaca ctaaaaaata tgagaaaatt tctggttctt ctgcttactt
    11881 tcttcatcca cggaaccatt ttctcccagg agtggctcac ttttgaagac agcagctcca
    11941 cttttaccgc caaatacccg gctacatgga caaaaaagat cagggctgac gggatcgttg
    12001 ttttttcatc gcccatatcc ggtgatacgg acaatttcaa ggaaaatatc aatgttattg
    12061 tccggaaaag cgctttattc accaaagatt ttgacctgaa ggaagcatta ccttccatca
    12121 tcgacaaact gaattcctcc ctgacggatt ttaagcaaaa ttccagccgt tttttcaaat
    12181 ggaacggtct ggatgccgcc gaaatcaagt tcactgtaac caataaagcg gaagatcagg
    12241 ataatccgct accgttaaaa atgatgcagt ggtattgttc cggcgaaggt gtattctaca
    12301 cggccacatt taccgcacat cgggataata accagtttga tgctgaggcg ttggaacttt
    12361 tccagagtat ccggttcaga taaccctacc ggattttgtc aacaaccggc taatcaagta
    12421 tctttataaa aaatactcct atgtcattgc tcaaccaggg aaacaaaaaa gacaagaaaa
    12481 aagaggataa gaaaaaaggg gccgccggct cgggttccaa attcatggct aagcccaagt
    12541 ccgggtttgt cagcaaacaa caaaataccg gttcccagcg gggtagttaa tcatataacc
    12601 agtatatgaa ccgggaagaa ctgctgaaag aactttcaga agaaacattt attaccctta
    12661 agccatcgcc tgtacacgga atcggggtgt ttgccctgcg ggatattccc aggggatgca
    12721 aaaccctctt ctcccgaaat gtcggggaat ggattcgggt gcccatttcc gatatcgacc
    12781 aattgccgga ccaaacccgc aatatggtgg aaacctactg cctgtacgat gaaacccact
    12841 attacctgcc ggattatggt ttcaaggtga tggacctggt caattacctg aaccattcct
    12901 ccacccccaa tgtaatctcc gtaaatgatg gtgaatattt tgaatcactc cgggaaataa
    12961 aagaagggga agaattgctg gtgaattatg gggagattgt ggatacgaaa gaatacctct
    13021 aagaaaagtt tcaagaacca agatgccaag agccaaagaa gcattaaaat tcaaatacca
    13081 aaaaagcaac aataaaaaat aaccaaaacc ttcttagaac ggtttggtgc ttatttgaaa
    13141 atattgcttt gaaagtataa actcctgcag tcaattaata tatcttagaa tgggctgttg
    13201 actaccgact actaactacc gactactaac ttacctttta cccggtaatg cgtcaccgcc
    13261 ctttccacct ggttgataat aagtccgctg atattcagct gccagctttg cttttcggta
    13321 tcaaaatagc aatcaatact gcctttggct accgaaatat aagaagactg gtcgccggtt
    13381 cggaaagaat aggccgcttc cacttcactt tttccggtag agcctgcctg aattttgtaa
    13441 gtcgtgttcc ttaatttttt ttcatgaaaa tataaagtga agaactggtc cttttgctct
    13501 aaccggatgg tgagcttgcc tttaacccgg tatactttca tcagtacagg ccgggcttcg
    13561 atgaaaaaat tattagccgt taccgtatcg gtaataaaaa caggacaatg agccagcgtg
    13621 gtatcgctgt ctgactgagc ataaacggca ctggaaaata ataagcagat tgaaatgagg
    13681 gcctggcgca tggttggtct ttgttggcaa ggaaaatacg aagaatttaa tgaatcagaa
    13741 tccctgtcat acaggccggt tccgctcgcc gaacagcagg cttccgatcc ggaccatatt
    13801 gccgccctct tccatagcga ttttgtaatc accgctcatg cccatggata gggtatcaat
    13861 ccggaaagcc ggggaactgt ctgatttata ctgatcgtaa aaatttttca attgcctgaa
    13921 ttcagctcgc acctgaaccg ggttatcgga gaaggaggcc atgcccataa acccgcggat
    13981 gcggatattc tttaaaggct gatcttttga ca
//
LOCUS       DAOPMW010000045        13892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4460_length_13892_cov_8.896798, whole genome shotgun sequence.
ACCESSION   DAOPMW010000045 DAOPMW010000000
VERSION     DAOPMW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_13892_cov_8.896798"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLU11_04055"
     CDS             complement(<1..648)
                     /locus_tag="PLU11_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN58318.1"
                     /translation="MSLFVKKPLDQILAQAADSEKGLKRTLGAGNLIALGIGAIIGAG
                     LFVRTAAAAGQAAGPAVTLSFVVAAIGCAFAGLCYAEFASMIPIAGSAYAYSYVTMGE
                     LIAWIIGWALIMEYALGAATVSIAWSEYLNKLLGGAIPYEWCHSPMESFTDTAGIHHS
                     GIMNAPALVILLALTLLLIKGTQESAFVNMIIVFIKVAIVILFIAIGWQFIKPENH"
     gene            complement(710..2155)
                     /locus_tag="PLU11_04060"
     CDS             complement(710..2155)
                     /locus_tag="PLU11_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN58319.1"
                     /translation="MAEHASSFKPTLGLFDGTMIVAGSMIGSGIFIVSADITRNVGSA
                     GWLIAVWLITGFMTLTAALSYGELSGMFPKAGGQYVYLKEAYNPLMGFLYGWSFFAVI
                     QTATIAAVGVAFSKFLAYLVPELGNNYFLFDAGIIKVYSGQLVAIAIICLLTYINSRG
                     VKEGKFIQTVFTSAKLFALFGLIAFGFLLVKESYWAENWKTGFTAVQDFGQKDATGQL
                     HPTAWMGISGSALLGAIAASMVGSIFSSDAWNNVTFIAGEMKNPKRNIGLSLFLGTLI
                     VTVIYVSANLMYLNVLPLNELAFPADDRVAVAAANKIFPSFGTILIAILIMVSTFGCN
                     NGLILAGARVYHTMAKDGLFFKQVGTLNKNAVPQVALWIQCIAASVWSLSGKYGQLLD
                     MISFVVVLFYMLTIIGIFILRKKRPDAERPYKAFGYPVLPFLYILMGAAFCILLIIFK
                     PEFTWPGLVIVLLGVPLYYLAISNRKKEQTG"
     gene            2297..3568
                     /locus_tag="PLU11_04065"
     CDS             2297..3568
                     /locus_tag="PLU11_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPN58320.1"
                     /translation="MSKTNKLTLYIFLAMLIGVGVGYWVHESYPKSTINVFSDNVKLL
                     TTIFLRLVQMIIAPIVFCTLVVGIAKLGDLKTVGRVGGKAMLWFITASLISLGLGLML
                     VNIFEPGVGVDVSNVDKAAVSELMDKSKGFSLAKFVEHVVPKSVVEAMATNEILQLVV
                     FSIFFGIALTALGEKGKGIIHTLDTLGHVMLKMVGYVMLVAPLGVFGAMASAIAKNGL
                     EILKTFGMYVGEFYLGLLILWMIMLIVGYIILKRRLPHLLRRIASPMAIAFNTASSEA
                     VYPKMVEEMERFGCKDKIVSFVLPLGYSFNLDGSMMYMTFASMFIAQAYGITSITGDV
                     GQQIIMLLVFLVTSKGIAGVPRASLVVVLATGQMFGLPAEGVGLILAIDVFCDMGRTM
                     TNVLGNALATAAVSKWEGALEHTDHHQHPHH"
     gene            3600..4337
                     /locus_tag="PLU11_04070"
     CDS             3600..4337
                     /locus_tag="PLU11_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPN58321.1"
                     /translation="MFLLDAFHWSQLVQPQWYVENGGLWLLLFVVFAETGLFVGFFLP
                     GDSLLFVAGIYAHEITKANEVAKPGLGYQVLKYLEISYPYTYWLDLIVLIGLISLMGI
                     LGNTVGYWTGRKVGPAMYHWRDRFLFKKKYLHQAHDFYEKHGGLAVIGARFLPFIRTF
                     APIVAGIVEMDRKKFHFYNVAGCILWVATMILGGFFLQKWVLQLFNFDLKEHLEFIVL
                     GIVLVTTAPVLIKMFSKHTPKTPTQTN"
     gene            4346..5581
                     /locus_tag="PLU11_04075"
     CDS             4346..5581
                     /locus_tag="PLU11_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN58322.1"
                     /translation="MDQAKPASIFNIAVIVAALGYFVDIYDLQLFGIIRVASLRDLGL
                     TEDQIRVEGESILQWQMVGLMIGGVIAGIMGDKKGRLSVLFGSIVLYSLANIANGFVE
                     TVEQYKWIRFVAGLGLAGELGVGITLVSEITKKESRGLATSLVAGIGLTGAVVAFIMK
                     ENFDWRTCYFIGGGLGMLLLILRISVYESGMFNAVKKTEVQRGNFFMLFTNWDRFKRY
                     LFGILIGLPTWYVIGVLVYFSSDFAREFGITEKVDPGKAIMYAYAAISLGDILIGLVS
                     QRLRSRKKALFIFYGITIIFMLLFFTIQWNGSAQKMYWICAGLGFGTGFWAIFVTMAA
                     EQFGTNLRATAATTIPNMVRGMLAIFILPLFKTLRGMEGMGYVKGGIYAGIIVMTITI
                     VAALLSKETFHKDMDYVEH"
     gene            5614..6630
                     /locus_tag="PLU11_04080"
     CDS             5614..6630
                     /locus_tag="PLU11_04080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN58323.1"
                     /translation="MEPADQIEKKAGKKFLIVRFSSIGDIVLTTPVIRCLKQQVPDAE
                     VHFLTRDRFHSVVEHNPYIDKVHQLAHSWELMIEELKTEDYDYIIDLHHNSKTLRLKN
                     ALKKKSFSFYKLNIQKYILTAFKINLLPKLHIVDRYMKTVESFGVKNDGKGLDYFISK
                     EEETQMHDIPVSHTAGYIACVIGAAHGTKRWPVHKWKEFCAQLNHPIILLGGPEDAAN
                     GNEIASVDDIKIYNACGKFKLNESADLVRKAKLVISNDTGLMHIAAAYQKIIISLWGN
                     TVPSFGMTPYYGDFSMTSLQMQTKVWCRPCSKIGYDKCPLGHFKCMEKIEVEEILKRV
                     RQKI"
     gene            7011..7946
                     /locus_tag="PLU11_04085"
     CDS             7011..7946
                     /locus_tag="PLU11_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58324.1"
                     /translation="MRLVIILLCLSGGLLSAQLPAARIDSFRLYKQVKVTIDRPVVLK
                     NGRPLALIFYALPNGNSTEWTMGKLKKEGDDWHYDIQHIRAQTAFIRQAMPRQNFVVI
                     YLENDFKSWPAMKTKFPDYKTMYAHILDTLAGLFPEKHQHIYLNGHSGGGSFIFGYLA
                     GVEKIPARIKRISFLDSDYNYDSSYYPKLKEWLVRVKDSHLNVFAYNDSVALYKGKRV
                     VSDSGGTWYRSRLMLRHLQRDFQFSESRTDSLLIYKSENNRIQFYLKPNYNQGIYHTQ
                     QVELNGFIHSVLCGTRKEGKRYEYYGKRAYGRLIE"
     gene            complement(8742..9503)
                     /locus_tag="PLU11_04090"
     CDS             complement(8742..9503)
                     /locus_tag="PLU11_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPN58325.1"
                     /translation="MKKATFIFSLLLISCLTSYGQIINVENSRLHSDTTGWMGGAGAS
                     INLNKNAVQVLGVNLESQLQYKAPSQKSIWLLLGNYSFLKAGENKLVSQGFIHLRYNY
                     KVNNWLRWEFFGQYQNNPVLFIDHRFLLGTGPRFRVFRHNKNRIYAASLLMLEDEKEL
                     TNPVISHTELRNSSYVSVSIIPNGQLELVNTFFYQPLLKKFEDYRFLNQLSVKVKAGR
                     HTSLGVKFNYLYDSKPAGTAPATTYNFATGIDFDF"
     gene            complement(9633..11516)
                     /locus_tag="PLU11_04095"
     CDS             complement(9633..11516)
                     /locus_tag="PLU11_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPN58326.1"
                     /translation="MHYVSVEGLTKSYGIQPLFSGISFHIEEGDKIALVARNGTGKST
                     LLRILAGQETADSGKLWINKEVDVVLFEQDPVFVEEKSVSDNIFFHDHPVINAIKEFE
                     AASETEDHERMSTSIVNMDELNAWDFDTKVKQVLGKLNIHHLEQPVNTLSGGQRKRVA
                     LAKTLIDIGFEHKHVLLIMDEPTNHLDVEMVEWLEHYLDKENVTLLLVTHDRYFLDNV
                     CNEIWELDGNSNMYVYKGDYANFLEKKAARIESEASSVDKARNLYRKELEWMRKQPKA
                     RTTKSKSRQDNFYEVEKKAKQQVADEQLQLEMKMSRMGGKIIELKKVYKSYGEKLILK
                     GFDYTFKKGERIGIVGKNGVGKSTFINMLQGLEQPDSGKINVGDTVIFGNYSQQGLIV
                     KEDMRVIEFVKNIAEHFPLASGGSLSAAQFLQLFLFDPDKQYTYISKLSGGERRRLHL
                     LSILFRNPNFLVLDEPTNDLDLPTLGVLENFLSEFPGCLLIVSHDRYFMDRLVDHLFV
                     FEGDGVIRDFPGNYTLYRNEIRDAVTVVEKKEVKTVPAPAPVTVATAPKRQPSFKEKR
                     EFELLEKEISDLTKERTTVTEKLNSGDTPFDELQKLSVRIGEIAVLLDEKEIRWLELS
                     ELM"
     gene            complement(11532..12182)
                     /locus_tag="PLU11_04100"
     CDS             complement(11532..12182)
                     /locus_tag="PLU11_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPN58327.1"
                     /translation="MQIKDYFALLELEASAPVTEIKKAYRRLALQLHPDKSGNDPYAA
                     ARFAEIKEAYEVLTDPSKKEYYLQQRWYNQTAGIRRTEQIVTPVTMIQQSLELERYVA
                     RLDEFRMDREGLLSFMLDLYSDERLEKLRAFQDTESMHTIVQSVIRSAKYLQPGQAEK
                     IAEQIRKLAPGDTVIARELADFLQHSRKKSNREKYSVWLALLLTALICLLIYLTSR"
     gene            12513..13271
                     /locus_tag="PLU11_04105"
     CDS             12513..13271
                     /locus_tag="PLU11_04105"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPN58328.1"
                     /translation="MKKTTTNTRRDFIKSTGKAGLAAGLSMTVIPALAKQYAGQKTAY
                     GWVEDVPYEQQPLGYAFPALEPVIDAMTMEIHYSKHAAAYAKNLADAVKAEGVDVSKE
                     KLVSLLGRISKFSPKMRNNGGGHYNHEFFWKSMRAASENNQPSGELAATITRDFGSFE
                     AFKTQFSDAGKNRFGSGWAWLVLNKEGKLVVGSTANQDNPLMDISDLKGTPLLGLDVW
                     EHAYYLKYQNRRPDYIAAWWKLVNWEVVSKRFGK"
     gene            13328..>13892
                     /locus_tag="PLU11_04110"
     CDS             13328..>13892
                     /locus_tag="PLU11_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN58329.1"
                     /translation="MLSTKINRLLLLVLSAGLFFYSCQREIKWPEQPALPNETAVNDA
                     ERVTGGVNGIVINENNQPMAGVTVRSGTNTTTTDRYGVFQFRNIQLSKANGTVSVEKN
                     GYFTGYRSFVAVEGLINNVRIKMLPKSNSGSFDAAAGGMVSLGSGAKLQMPANSVTDA
                     AGTAYTGNVNVAMTWIDPSFVDLPFNMM"
BASE COUNT         3580 a         3135 c         3426 g         3751 t
ORIGIN      
        1 gtggttttcc ggcttgataa actgccagcc aatcgcgatg aataaaataa cgatagccac
       61 tttgatgaat acgatgatca tatttacaaa tgcagactcc tgggtgcctt tgatcagcaa
      121 cagggtcagc gccagcagga tcaccagggc gggggcgttc atgataccgg agtggtgtat
      181 accggcggta tcggtgaaac tttccatcgg cgagtggcac cattcatacg gtatggcccc
      241 tcctaaaagc ttatttaaat attcactcca ggcaatagaa actgtggcgg caccgagggc
      301 atattccatg atcagcgccc agccaataat ccaggcaatc agttcaccca tggttacata
      361 agaataggca tacgcactac ccgcgatggg gatcatagaa gcaaattccg cataacacaa
      421 accggcaaaa gcacaaccaa tggccgctac gacaaaggaa agggtaactg cgggaccggc
      481 tgcttgtccg gccgctgctg ctgtacgcac aaaaagacct gcaccaataa tcgctccgat
      541 gcccagcgcg atcaggttgc cggcacctaa tgttcttttc aagccctttt cagaatcggc
      601 cgcctgcgcc agaatctgat ccaatggctt cttgacaaat aaactcatga gttggttttt
      661 agtgagtgac taagataagt attgattaaa tacgaaacag agactttttt taaccggtct
      721 gctccttttt tcgattggat atagccaggt aatataaggg tacacccagt aatacaatga
      781 ctaagcccgg ccaggtaaat tccggtttga aaatgatgag cagaatacag aaggcggctc
      841 ccattaatat gtataaaaat ggtaatacgg gatagccaaa agccttgtat ggtcgttcgg
      901 catcgggtct ttttttccgt aaaataaaga tgccaatgat cgtgagcata taaaataaga
      961 ccactacaaa ggagatcatg tccagcagct gaccatattt tccactcaga ctccagacag
     1021 aagcggcaat acactggatc cataaagcta cctgcggtac cgcattttta ttcagggtgc
     1081 cgacctgttt aaaaaacaac ccgtccttgg ccatggtatg atacacccgg gcaccggcca
     1141 ggatcagtcc gttgttacac ccaaacgtag acaccatgat caggatggcg atcagtatgg
     1201 taccaaaaga agggaatatt ttattggcgg cggccacggc cacccggtca tctgcgggga
     1261 aggccagttc attcaagggc agcacattta aatacatgag gttcgccgat acataaatga
     1321 ctgtcacgat cagggtgccg agaaataaac tcagcccgat attccgcttg gggttcttca
     1381 tttccccggc aataaaggtc acattgttcc aggcatcact gctgaaaata gaaccgacca
     1441 tggaagcggc aatggctcct agcaaagccg aaccggaaat gcccatccag gcagtgggat
     1501 gtaattgacc ggtggcatct ttttgcccga agtcctgcac cgctgtaaaa cccgtcttcc
     1561 agttctccgc ccagtaactt tcttttacca gcaggaaacc aaatgcgatc agtccgaaca
     1621 gggcaaataa tttggcggaa gtaaaaaccg tctgaatgaa ttttccttcc ttcacccccc
     1681 tggaattaat ataagtcagc aggcagatga tggcaatagc cacgagctgc ccggaataca
     1741 ctttgataat acccgcgtca aacaggaaat aattattgcc cagttcaggt accaggtaag
     1801 ccaggaattt ggagaaagcc acacccacgg ccgcgatggt agcggtctgg atcacggcga
     1861 aaaaactcca gccatagagg aatcccatca ggggattata ggcttctttg agatagacat
     1921 attgtccccc ggcctttgga aacataccac tcagttcccc ataactcagg gcggcggtca
     1981 gggtcatgaa gccggtgatc agccatacgg cgatcagcca gccggcacta cccacattcc
     2041 gggtgatatc ggcactcaca ataaaaatac cggacccgat catggagccg gctacaatca
     2101 tggtgccatc aaaaagaccc agtgtgggct taaaggaact tgcgtgttct gccatacaat
     2161 cagttttgaa gccttggtta aaaggggaaa atactgattt ccggttaatc gcttaaaaaa
     2221 gagttattaa cggcggtttt ttaatttttc aactgggtaa ttttaccgat tttcggacat
     2281 atttaatcag actgctatgt cgaaaaccaa taaactgacc ctctatattt tcctggctat
     2341 gctgatcggt gtgggagtgg ggtactgggt gcatgagtcc tatccgaagt ccactatcaa
     2401 tgttttttcg gataatgtaa aactcctgac caccatcttt ctgcgcctgg tgcagatgat
     2461 tattgccccc attgtattct gtacgctggt tgtggggatt gccaaactgg gcgatctgaa
     2521 aaccgtggga cgggtaggag gcaaggccat gctctggttt ataacggcct ccctgatatc
     2581 gttgggtctc ggactgatgc tcgtgaatat ttttgagccg ggtgtagggg tggatgtgag
     2641 taatgtggac aaagctgcgg tgagcgaact gatggataaa tcaaaaggat tttcattggc
     2701 caagtttgtg gaacatgtgg tgcccaagag tgtcgtggaa gccatggcca ctaatgaaat
     2761 tctgcaactg gtggtgttct ccattttctt tggtattgca ctgactgcct tgggtgaaaa
     2821 gggcaagggg attattcata cactcgatac tctcggtcat gtgatgttga aaatggtggg
     2881 ctatgtgatg ctcgttgccc cattgggtgt gtttggcgct atggcatctg ccattgcaaa
     2941 aaacggcctg gaaatactga agacatttgg tatgtatgtc ggtgagtttt acctggggtt
     3001 actgatactc tggatgatca tgctgatcgt gggctatatt atattaaaaa gaagactgcc
     3061 ccatctgctt cgtcgtattg ccagtccgat ggccattgcc tttaatacgg ccagcagtga
     3121 agcggtatat cccaagatgg tggaagagat ggaacgcttt ggttgtaaag acaagatcgt
     3181 gtcgtttgtg ctgccgctgg gttattcgtt taatctcgat ggcagtatga tgtatatgac
     3241 ctttgccagt atgttcatcg cacaggctta tggcattacc tctattacag gggatgtagg
     3301 tcagcagatc atcatgctgc tcgttttcct cgtcaccagt aaaggaattg ccggggtacc
     3361 ccgtgcctct ctcgtagtgg tactcgctac cgggcagatg tttggtttgc cggcagaagg
     3421 ggtgggactg attttagcca tcgatgtgtt ctgcgatatg ggccgtacca tgaccaatgt
     3481 actgggcaat gccctggcca cggcggcggt aagtaaatgg gaaggcgcat tggaacatac
     3541 cgatcatcac cagcaccccc atcattaatt cactcatgtt attaaaataa aggaactata
     3601 tgtttttact ggatgcgttt cattggtcac agctggtaca gccgcaatgg tatgtggaga
     3661 atggcggact ctggcttttg ctttttgtgg tgtttgccga aacaggatta tttgtcggat
     3721 tttttctgcc gggagattcc ctgctctttg ttgcgggtat ttatgcccat gaaattacca
     3781 aggccaatga agtggccaaa cccgggttgg gttaccaggt tctgaaatac ctggaaattt
     3841 cctatcccta tacctactgg ctcgacctga tcgttttgat cggtctgatt tctttaatgg
     3901 gaattttagg aaatacggtc ggttactgga ccggccgtaa agtgggtccg gccatgtatc
     3961 attggcggga ccgtttttta tttaaaaaga aatacctgca ccaggcacac gatttttatg
     4021 agaagcatgg cggactggcc gtgatcggtg cccgtttcct gcccttcata cggacctttg
     4081 ctcccatcgt tgccggtatt gtggaaatgg atcgtaagaa attccatttc tataatgtgg
     4141 ccggttgtat tttgtgggta gccaccatga tccttggcgg gttcttcctg caaaaatggg
     4201 tgctgcaatt gttcaatttt gatttaaagg aacacctcga atttattgta ctgggtatcg
     4261 tattggtcac cactgcaccg gtgctgatta aaatgttttc caagcatacc cccaagaccc
     4321 cgacacaaac caactaacga ttcatatgga ccaggccaaa ccggcgagca ttttcaacat
     4381 tgcagtgatc gtggccgccc tcggctactt tgtcgatatc tatgacctcc agttattcgg
     4441 tattatccgc gtggccagtc ttcgcgacct gggactgacc gaagaccaga tccgtgtcga
     4501 aggcgaatcc atcctgcaat ggcagatggt aggcctgatg atcggcggtg ttattgccgg
     4561 tatcatgggt gataaaaaag gaagactcag tgtgttgttt ggttccatcg tcctttattc
     4621 cctggccaat atcgcgaatg gatttgttga aaccgtagag caatacaagt ggatacggtt
     4681 tgtggccgga ctcggactcg ccggtgaact gggtgtagga attaccctgg tatccgagat
     4741 caccaagaag gaaagccgcg gactggctac ttcgctggtg gcgggtatcg gactcaccgg
     4801 ggccgtcgtg gcttttatta tgaaagaaaa tttcgactgg cgcacctgtt attttatcgg
     4861 gggcggtctg ggtatgttat tactcattct ccgtatctcc gtttatgaat ccggtatgtt
     4921 caatgcggtg aaaaaaacag aagtacagcg gggaaatttt ttcatgctgt tcaccaactg
     4981 ggaccgcttc aaacgttatt tattcggaat cctgatcggc ctgcctacct ggtatgtgat
     5041 cggggtgctg gtatatttct cctccgattt tgcccgggaa ttcgggatta ccgaaaaagt
     5101 ggatccgggt aaggcgatca tgtatgctta cgcggccatt tcattgggtg atatcctgat
     5161 cggtttagtc agtcagcgcc tgcggagccg gaagaaagca ctctttatat tttacggcat
     5221 taccatcatc ttcatgttgc tcttctttac gattcagtgg aatgggtcgg cacagaagat
     5281 gtactggatc tgtgccggac tgggttttgg taccgggttc tgggccatat ttgtcaccat
     5341 ggcggccgag cagtttggta ccaatctccg cgccactgcc gctaccacca ttcccaatat
     5401 ggtacgggga atgctggcta tctttatatt gcctttgttc aagacattaa gaggaatgga
     5461 agggatgggc tatgtgaagg gtggtattta tgcgggtatt attgtaatga cgattaccat
     5521 cgtggcggcc ctgctcagca aagagacctt ccataaggat atggattatg tggagcatta
     5581 aataattatc gttttaaagg agatcattcc gaaatggaac cagccgatca aatagaaaaa
     5641 aaagccggaa agaaattcct cattgtacgc ttctcttcca ttggtgatat cgtgctcacc
     5701 accccggtga tccgttgcct taaacaacag gtgccggatg cggaagtaca ttttcttacc
     5761 cgggatcggt ttcattccgt ggtagagcac aatccctata ttgataaagt ccaccagttg
     5821 gcccatagct gggaactgat gatcgaagaa ctgaagacgg aagactatga ttacattatc
     5881 gatctgcacc acaacagcaa aacactgcgg ttaaaaaacg cgttgaagaa aaaatccttc
     5941 tctttttaca aactcaatat tcagaagtat atcctgacgg cctttaagat taatctgctg
     6001 cccaagttgc atattgtgga ccggtatatg aagacggtgg aatctttcgg agtaaagaat
     6061 gacgggaagg ggctggatta ttttatcagc aaagaggaag aaacgcagat gcatgatatt
     6121 cccgtctccc atacggcggg gtatatcgct tgtgtgatcg gcgcggccca tggcaccaag
     6181 cgctggccgg tgcataaatg gaaggaattc tgtgcacagc tgaatcatcc catcatcctg
     6241 ctgggcggac ccgaggatgc ggccaacggg aatgagatcg cttcggtaga tgatatcaag
     6301 atttacaatg cctgtggcaa attcaaactg aatgaaagcg ccgacctggt acgcaaggcc
     6361 aaactggtaa tcagtaatga taccgggctc atgcatatag cggccgcgta tcaaaaaata
     6421 atcatctccc tttgggggaa tactgtgcct tcttttggca tgacgcctta ttatggggat
     6481 ttttccatga cctctttaca aatgcaaacg aaagtttggt gccgaccctg ttctaaaatc
     6541 gggtacgaca aatgcccgct gggacatttc aaatgcatgg aaaaaattga agtggaggag
     6601 atacttaaaa gagtcaggca aaagatatag ctcctcctca aattgagaag acttttgcct
     6661 ggctcttaca atactttaca agagtctgca gcagtgttaa ctgttcaaaa agaataatgc
     6721 cagcagcgcg aaagtaacga ggaatccacc gattactaaa cgggcctgca catcttcatt
     6781 cttcattaag agtttaattt tcttcataac ctgatttttt aaaagttttc aaatagaaat
     6841 actaccaggt tttcagggga ctggacaagt caatcgttag gataacgaat taagtggggt
     6901 aatgcaaaag tagactacct ttttatatcc tgcaagaatg gggccggatt ttttgattag
     6961 gaaatctacg atagctgcta ttcccttgaa ggacttacct ttagagtgct atgcgactgg
     7021 taattatact actatgcctt tccggtggac tgctttctgc ccagttgccg gctgcccgga
     7081 ttgactcttt tcggttgtac aaacaggtga aagtgaccat tgaccgtccg gttgtgctga
     7141 aaaatggccg tcccttagcc ctgatttttt atgcccttcc caacggaaat tccacggaat
     7201 ggaccatggg aaagctgaaa aaggaaggag atgactggca ttacgatatt cagcatatcc
     7261 gggcgcaaac ggcctttatc cggcaggcaa tgccccggca gaattttgtg gtaatctatc
     7321 tcgagaatga tttcaaaagc tggccggcga tgaaaacgaa gtttccggat tataaaacaa
     7381 tgtatgctca tattctggac acactggctg gcttgtttcc cgaaaagcat cagcacattt
     7441 acctgaacgg acatagcggg ggtggcagtt ttatttttgg ctacctggcc ggcgtggaaa
     7501 aaattccggc aaggatcaaa cggatcagtt tcctggacag tgattataat tatgacagca
     7561 gctattatcc caaactgaag gaatggctgg tccgggtaaa agacagtcac ctgaatgtat
     7621 ttgcatacaa cgacagcgtg gctttataca aggggaaaag agtggtatcc gattccggcg
     7681 gtacctggta ccggagccgg ctgatgttgc gtcacctgca aagggatttc caattcagcg
     7741 agtcccgtac agacagtctt cttatttata aaagtgaaaa caaccgaata cagttttacc
     7801 tcaagcctaa ttacaatcag ggcatttatc atacgcaaca ggtggagttg aatggcttta
     7861 tacattccgt gctttgtggc acgcggaaag aggggaagag gtatgagtat tatgggaaga
     7921 gggcgtatgg gaggttgatt gagtagttgt cgggtgtcgg gtgtcaggtg tcaggaaaca
     7981 gcccgtatga gaaggtgtta gctgttagct gttaggaaac agcccctgct aagaaatatt
     8041 agtatagaag aagacaggca atgaagtaat gttcttgact ggccccgtag cttttagctg
     8101 cggggtttta tgttgtcgct tttagcgacc tgagaaaatg tacaggtgcc tgctcaacga
     8161 ggcttgtagc ctcgttctcg ttgtatttgt tttgatatga tgaataggcc tattgatcag
     8221 acagcgaccg ctacagaggg agattgcttg ataattgcaa attgcaattg tgtcacccct
     8281 acggggcttt cgggcatctt catttgatcc aggctataat tgtgacaccc ctacggggct
     8341 gggggagggg acagataata gtatggcgta agataatcat gtcacccctt cggggtgagt
     8401 ctgtctcgaa tatatccaac tattcttctg ctatagaaat ttcacccctt cggggttata
     8461 gagttccgtg gggggcacct taccccgggt tggcacccgg ggctatgaat cgttcaaccc
     8521 ctttggggtt ggtagtatat tatttgtatc gcggtacttt taatcatcaa gagaggaatg
     8581 ttcgtgtaat cctgcccgcc gaccgaagcg tagcgaagat gggagtagtc ccgcaaacaa
     8641 acatcaccgg gctggtgata acagtctctc gggaagtatc gaagaatgaa ctcggccact
     8701 caccattccc cactccccat tccccattca ccatcccctc ctcagaaatc aaaatcaatt
     8761 cccgttgcaa aattatatgt agtagccggc gccgttccgg ccggctttga atcatagaga
     8821 taattaaatt ttacgccaag cgaggtatgt ctgccggctt ttacttttac agagagctga
     8881 ttcaggaacc ggtagtcctc aaatttcttc aataagggct ggtaaaaaaa agtgtttacc
     8941 agttccagtt gtccgtttgg aataatggat acggacacat aactgctgtt gcgcagttca
     9001 gtgtgactga ttaccggatt ggttagttct ttctcatctt ccagcatgag tagtgacgcg
     9061 gcatagatac ggtttttatt gtggcggaaa accctgaatc tcggtcctgt tcccagtaaa
     9121 aaacggtgat caataaaaag caccgggtta ttctgatact ggccaaaaaa ctcccaacgc
     9181 agccagttat tgactttgta attatagcgg agatggataa aaccctgtga aacgagcttg
     9241 ttttcccccg ctttgaggaa actgtagttg cccaataaga gccagatact tttttgggaa
     9301 ggggctttat attgcagttg cgattccagg ttgactccca gtacctgcac cgcattttta
     9361 ttaagattaa tagaagcacc ggccccgccc atccaaccgg tagtatccga atggaggcgg
     9421 gagttttcga cgttgatgat ttggccgtaa ctggtcaggc agctgatcaa cagcagggaa
     9481 aaaatgaagg ttgctttttt cattgaaaaa tacattaaaa ggtgtcaggt gtctgctgtc
     9541 aggtgtcagg aaacagcccc ttctaacaac ataaacttca aggcacgtca gccgtacttc
     9601 gtacttcgta cctcgtactt tgtatttcgt acttacatta attcgcttag ctccaaccac
     9661 ctgatctcct tctcatccag caacaccgct atttccccaa tccgcacgga tagtttttgt
     9721 aattcatcaa aaggtgtatc accactgttc agtttttcag tcaccgtagt tctttctttc
     9781 gtcaggtcac tgatttcttt ttccagtaat tcaaattctc ttttttcctt aaaactgggt
     9841 tgccttttcg gggcggttgc tacagttacc ggggcagggg cggggacggt tttcacttcc
     9901 ttcttttcca ctaccgtcac cgcgtcgcgt atctcattgc gataaagggt gtagtttccg
     9961 gggaagtcac ggatcacgcc atcgccttca aagacaaata agtgatccac gagccggtcc
    10021 ataaaatacc ggtcatgcga tacaatcagt aaacagccgg ggaattcact taagaaattt
    10081 tccagcacgc cgagggtggg gaggtccagg tcattggtcg gttcatccag aaccaggaaa
    10141 ttgggattac ggaacaatat cgataataaa tgcagtcttc tccgttcacc tccgctgagt
    10201 ttactgatat aggtatactg cttatcggga tcaaataaaa acaattgtaa aaactgggcg
    10261 gcgcttaatg agccgccgct ggccaggggg aaatgttcgg cgatgttttt tacaaactca
    10321 atcacccgca tatcttcctt cacgattaat ccctgttgtg aatagttgcc gaaaataacc
    10381 gtgtcgccta cattgatctt cccgctgtcg ggttgttcaa gtccctgtaa catgttaatg
    10441 aaagtcgatt tccccacgcc gttcttaccg acaatcccga tgcgttctcc ttttttgaaa
    10501 gtgtaatcaa accctttcag gattaacttc tcgccataac ttttatatac ttttttcagt
    10561 tcgatgattt tgcctcccat ccggctcatt ttcatctcca gctgtaactg ctcatccgcc
    10621 acctgttgtt tggccttttt ctctacttca taaaaattat cctgacggct cttgctcttg
    10681 gtggtacggg ccttgggttg tttccgcatc cattccagtt ccttgcggta caggttgcgg
    10741 gccttgtcca cactggaggc ctcgctttcg atacgggctg cctttttctc caggaaattg
    10801 gcatagtcgc ctttgtacac atacatgtta ctgttgccgt ccagttccca gatctcatta
    10861 catacattat ccaggaaata acggtcgtgg gtcaccagta acagcgtcac attttcttta
    10921 tcgagataat gttccagcca ttccaccatt tccacatcga ggtggttggt cggttcgtcc
    10981 atgatcagca acacatgctt atgttcaaaa ccgatatcga tcagggtttt ggccagggct
    11041 acccgtttac gttgtccgcc acttaaggta tttacgggct gctcgaggtg atgaatattc
    11101 agtttgccca atacctgttt tactttggtg tcaaaatccc aggcattgag ttcatccata
    11161 ttcacaatgg aggtactcat gcgttcgtga tcttcggttt cactggcggc ttcaaactcc
    11221 ttgatcgcgt tgatcacggg gtgatcgtga aagaaaatat tatctgaaac ggatttttcc
    11281 tcgacgaata ccggatcctg ttcaaacaat acgacatcta cttctttatt gatccagagt
    11341 ttaccggagt ccgcggtttc ctgtccggcc agtatgcgga gcagggtgga tttacccgtg
    11401 ccattccggg ccaccagggc aattttatca ccttcttcta tatgaaagga aatgcccgaa
    11461 aaaaggggct gtatgccata actcttcgtc aatccttcta ctgaaacata atgcatgggg
    11521 taaagataag tttacctgct ggtaagatat atgagtaaac agatcagggc ggtcagcagc
    11581 agggccagcc acactgaata tttttcacgg ttacttttct tccttgaatg ttgcagaaaa
    11641 tcagccagtt cacgggcaat caccgtatcg cccggtgcga gtttccggat ttgttcagct
    11701 atcttttctg cctgtccggg ctgcaggtat tttgcggacc tgatcaccga ttgtacaatg
    11761 gtatgcatgc tttccgtatc ctggaacgcc cgtagttttt ccaggcgttc atcggaatag
    11821 aggtccagca taaatgacag taacccttcc cggtccatcc ggaattcgtc cagccgcgcc
    11881 acatagcgtt ccagttcaag ggattgctga atcattgtga ccggcgttac gatttgttct
    11941 gtacgacgga taccggccgt ctggttgtac cagcgttgct gcaggtaata ctcttttttg
    12001 gagggatccg tcagcacttc ataggcttcc ttgatctccg caaagcgggc cgcggcatag
    12061 ggatcattgc ccgatttatc cggatgcagc tgtaaagcca gcctgcggta ggcttttttt
    12121 atttcagtca ccggggcaga agcttccagt tccagtaagg cgaaataatc tttaatctgc
    12181 atcgggcgcg aagatacggg caggaggggt ggtaatttaa cagaaattgc aacctcttta
    12241 cccggtttta agtcagagaa agtggaatta aaggtaactt agagcgttgt ggaggcccgg
    12301 agtaaggctt tctaagtacg aggtacgagg tacgaagtac gaaagggatg tcgtaaaacg
    12361 aggaaatgtt tgttgcttaa gatagacctt ctaaagaggg gctgttgact accgactacc
    12421 gactaccgac tactaactcc cgactgattt gggaggggct gtttcctgac acctgacagc
    12481 agacacctga caccttttaa agtaaatgca caatgaaaaa aacaactacc aacacccgcc
    12541 gcgattttat caaatccacc ggcaaagccg gcctggctgc cggattgtcg atgaccgtaa
    12601 taccggcatt ggccaaacag tatgcaggac agaaaaccgc gtatggctgg gtggaagatg
    12661 tgccttatga gcagcagccg ctgggctatg cctttccggc gttggaaccc gtgatagatg
    12721 ccatgaccat ggagatccat tacagtaaac acgccgctgc gtatgccaag aacctggcgg
    12781 atgccgtgaa ggcagaaggc gtggatgtgt caaaagagaa actggtttca ttattgggaa
    12841 ggatctctaa gttctctccc aaaatgcgca acaatggtgg cggtcattat aaccatgaat
    12901 ttttctggaa gtccatgcgg gcagccagtg aaaataatca gccttccggg gaactggctg
    12961 caacgattac ccgtgacttc gggtcattcg aagcattcaa gacccagttc agcgatgccg
    13021 gtaagaaccg ttttggcagt ggatgggcct ggctggtgct gaataaggaa ggcaaactgg
    13081 tcgttggttc tacggccaat caggataatc cgttgatgga tatcagtgat ttaaaaggta
    13141 caccgctatt aggactggat gtatgggaac atgcttatta cctgaagtac cagaaccgtc
    13201 gtccggatta tattgcggcc tggtggaaac tggtgaattg ggaggtggtg agcaagcgtt
    13261 ttggcaaata agcggattaa tcactgtcat tgcctgattt gacttatttt attattttgt
    13321 ctgatatatg ctaagtacca aaatcaaccg actgctcctg ctggttttgt cagcaggact
    13381 cttcttctat tcctgtcagc gtgaaatcaa gtggcccgaa cagccggcgc tgcccaatga
    13441 gacagccgtc aatgatgcag aacgcgtgac tggaggggtc aatggaattg taataaacga
    13501 aaacaaccag cccatggctg gtgtaacggt caggagtgga accaatacca cgactacgga
    13561 ccgttatggt gtgtttcaat tcagaaatat ccagttgtca aaagccaacg gcaccgtcag
    13621 tgtggagaaa aacggttatt tcacggggta ccggagtttt gtagcggttg aaggacttat
    13681 caataatgtg cggattaaaa tgctgcccaa atccaacagt ggcagttttg atgcggctgc
    13741 cggtggaatg gtgagtctgg gttcaggtgc caaactacaa atgccggcta attccgtgac
    13801 ggatgcagcg ggtacagcct atactggtaa tgtaaatgtg gcgatgacct ggattgatcc
    13861 atcatttgtg gatcttcctt ttaatatgat gg
//
LOCUS       DAOPMW010000046        13783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4510_length_13783_cov_8.842512, whole genome shotgun sequence.
ACCESSION   DAOPMW010000046 DAOPMW010000000
VERSION     DAOPMW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_13783_cov_8.842512"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLU11_04115"
     CDS             <1..384
                     /locus_tag="PLU11_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPN58330.1"
                     /translation="RNNGVLISKFQDKTTGYSIDKLQDRGTFFVDPGEEVYCGQIIAE
                     NIKPGDLVVNATEGKKLTNHRASGSDDATRIAPKTQMTLEECMEYIQQDECIEVTPNF
                     IRMRKVILNEDDRKKVQKRMEADAV"
     gene            806..2887
                     /locus_tag="PLU11_04120"
     CDS             806..2887
                     /locus_tag="PLU11_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58331.1"
                     /translation="MPFRILFLFLLGFSCTAIQAQCLSGNCTDGFGKFKYKNDNIYEG
                     NFKGGYCNGKGKMFYSNGEFYEGDWVNGTWNGKGLYTWKDGRRFEGQVKDGYFDGFGI
                     YFMTDSNRYEGNWKVGSFEGTGKYFFKDGRYYDGGWLKGKKSGKGKFTWPDGGSYIGD
                     WKEDKFEGEGKRIYNDSSEYEGSWVASVQQGKGRYVYNNGDVYAGEFKEGKRSGLGKY
                     VVKADGQVYEGTYLNGQPEGKGKLVMGNKDRYDGDFKNGLADGYGIGEYTTGKRYEGE
                     WKEGNWNGKGKATWKDSSSYEGQWVKGKMQGTGTKIYKGGDRYTGNWFEDKIEGQGEY
                     TWKNGDVYTGHFTNEKMSGQGKKVYKDGSVYEGSWLFGLYSGKGVLSWPNKNRYEGNF
                     STGVALGNGAFYRNGVLLFEGNFNGTSTYSTATTWLLTDGRFARRTTYTDGSIFEIWH
                     PDQSMFIGSIIDIGYNGSISEIKPREGSLLYATGKVISGKWEDGTIKQPTYQYQMKMS
                     EAVPEYVKGRIYYAYSKYADALKSFNKAIGGNLKEDSVYLFRGAAYYGLKLYDSARAD
                     LTTLLKKQAKNKEGITWRAKTLLSLKDTTAALADFSTYIKNFPGDSTGYWQRGVIYHN
                     VKNYDAAVKDYTTYLQRTKARNENVYYYRGICYEHLGKKTEACADFETAKKGGNKNAD
                     AQIKKLCGNPG"
     gene            2959..3180
                     /locus_tag="PLU11_04125"
     CDS             2959..3180
                     /locus_tag="PLU11_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58332.1"
                     /translation="MKKSLIIGLVIVAGLLLAGPEAVAQCSICTKTAQQLGEGPAKGM
                     NTGILYLAFAPMAIIGFLAYRWIKNNRQA"
     gene            complement(3181..5073)
                     /locus_tag="PLU11_04130"
     CDS             complement(3181..5073)
                     /locus_tag="PLU11_04130"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN58333.1"
                     /translation="MKNRNRSIRVFFVFVLWFHFQSVLIPASGQTTAISGVVNSYYRV
                     LEIIPAKACIRLSSTAGLTVNDPILLIQMKGATITTTNNSSFGTVTALNDAGNYETGT
                     ICSIIGDSVFLFHILENTYTPATGKVQLVPFARYVSANVTDTVKATPWNNSTGTGGVI
                     AIYTEQDLILNAPVYAGASGYRGGNFQQSNGTCSDALPASGYAYNASNLSPQSGAAKG
                     EGIADVASNQTGGRGAPANGGGGGNNHNNSGGGGANLNAGGTGGGNSSSGVACTATLK
                     GEAGRSLSSNSGLKIYAGGGGGAGHSNNGLSNVSGANGGGIIFIWANNLLGNSQSINA
                     SGGSGGSSLSDGAGGGGAGGTIILHISNYTGPVTIEANGGNGGNSNNGGNIGRCFGGG
                     GGGSGGIIYFTGSLPAITTSVTGGAAGVESGRDVSCNPVPQSAAAGTAGQVIPDYTFT
                     RSFSPASYCSLILPADFITFNATIVRENVQLKWEVTDPELISHFGIERKTTNGNWEFI
                     GEKQAIDGQSVYQLTDPLTITGHHYYRVRMIRKDQSAVLSAIKAVHIKPATAFTFYPN
                     PANTELFILRKQAATAVLKLSDLSGRTLLQEEVRSTRYRLVLPVLPAGIYLLNMDGEI
                     KKLQIR"
     gene            complement(5109..6005)
                     /gene="meaB"
                     /locus_tag="PLU11_04135"
     CDS             complement(5109..6005)
                     /gene="meaB"
                     /locus_tag="PLU11_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPN58334.1"
                     /translation="MWQELLAQVQQGNSKALARSITLVENEHPGYEKLLQSLPPSHSK
                     IIGITGPPGAGKSTLVDALIGVFVGKGRKVAVVCVDPSSPFNLGAVLGDRIRMSDWYT
                     NDNVFIRSLATRGSMGGLHPKIIEITDLLKAFRFDYIIVETVGVGQSEVEIAGLADVT
                     AVVVVPEAGDEIQTMKAGLMEIADLFVVNKADRPDADMFVRNLRLMLAPSFSRKTAEV
                     PVIKTVAARKEGINEFAEKIEELLVSSGSNEKTSWLLAEKAYYLIGQKRMKDISKSRL
                     KAEIETALEQGNFNLYQYIAGK"
     gene            complement(6054..6824)
                     /locus_tag="PLU11_04140"
     CDS             complement(6054..6824)
                     /locus_tag="PLU11_04140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN58335.1"
                     /translation="MNKLSVAIITFNEERNIGRCIDSVLSVADDVIVLDSHSTDNTCA
                     IAVAKGARVVQQAFAGYIEQKNKALALAEYDLVLSLDADEALDERLQQSILSAKNNNP
                     AAGYTMNRCTYYCGRFIRHGAWYPDRKLRLFNKQLAIWGGTNPHDKVEFESPQPTVHL
                     TGDILHYSYYSIEEHITQNNKFSTISAESYFNRGKKTSLFKIFFNPCWAFFSGFILRG
                     GFRDGFYGYVIARNVAHLTFLKHSKLYLLQRNAAEGKK"
     gene            6889..7662
                     /locus_tag="PLU11_04145"
     CDS             6889..7662
                     /locus_tag="PLU11_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HPN58336.1"
                     /translation="MKIPNQTIAFLEKYFDRIFVITLERAGDRQVKVKERLEGLTFEF
                     FYGVDKHGLSWEKVNQEGVYDDKKARQLNRYSKGMVLGHIACALSHRKLYQHIVEKGY
                     QRVLIFEDDAVPLFDKDGELQQSVEELPANWEVIYFGYNKNETATPALKRKQAFYRVI
                     SHLKLIKWSPLMVSNLLPKPYSPHLQKAGFHDLTHSYAVTREACLKLIRAQTPVVFNA
                     DPLVSHLIMNGELSAFITRKKFFTQEQFLDPAHRSFIHH"
     gene            complement(7663..8535)
                     /locus_tag="PLU11_04150"
     CDS             complement(7663..8535)
                     /locus_tag="PLU11_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN58337.1"
                     /translation="MKMSGFTIIRNAVLNDYPVVEAILSVLPVVDEMIVSIGDSEDET
                     EALILSIGNPKIKIIHSVWDPELKKGGKVLAVETDKAKAACAPDSDWLFYIQADEVIP
                     EQYQAAIRDAALMYKDNKKVEGLLFHYLHFYGTYDYVGDSRKWYTKEARIIRNDKTIT
                     AFRDAQGFRKGNTRLNVKQIPAWVFHYGWVKSPELMKRKLKNTGVFWHGEEAGFQEFL
                     KTEDFFDYNDFDSLKKFLGKHPAVMKERVSKGNPAVQLDITQKKFSLKKRLLYWFEKK
                     TGIRPFSFRNYRII"
     gene            8616..9824
                     /locus_tag="PLU11_04155"
     CDS             8616..9824
                     /locus_tag="PLU11_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN58338.1"
                     /translation="MKPDALLSPRYLSLVLAALLVTGLLFSRALLSIMMASGLLLLLF
                     PSVRQVFGAVFKRPLLFLLAGIIIFYAWLFFLDPQQYTLERMGLHLGVISLLCLLHFM
                     AATSDWLLPFILSVGVLSTFPSVYDMVMHEGLAALYEKGQVAYTLMQGDHQRFTIWIS
                     GCLAFSWYYWLMEHKKQAFYFILYFTIFLLLLSVRTGWLFAGLVTVAGGLIYLQRQHK
                     RVYWIRAILGLILLLAIAYSVPFVRNKIQYMQWEWREKKAAEQLGSSDAIRWMVNESA
                     VELIRENPKGLGEAKAALALKERIKINYPESSVSFQWPFNQYLKWWLTAGWVMGSLPL
                     LLVLYLLYRLYFTRNYLTAVWVLFIALTGLYESTLEMQYGFFLGVFFTGLLYLYESGL
                     SKRTVLRAYR"
     gene            10030..10668
                     /locus_tag="PLU11_04160"
     CDS             10030..10668
                     /locus_tag="PLU11_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPN58339.1"
                     /translation="MTNFIPIFPLGLVVYPGESLNLHIFEPRYKQLIQECHAAKKPFG
                     IPTVINNKLQEFGAMVEIDELTKIYDNGEMDVKTKGLRIFRILELIKEIPDKLYSGAI
                     VTYPDNYGQGNPDTMRKLMTSIRELHGLLKLSKDFKKTDEEMLSYDVAHHIGLSLDEE
                     YELMGLMDEKQRIAYIKRHLIKVIPMVSGLEQLKEKIKLNGHFKNLSGFDIQ"
     gene            10716..11441
                     /locus_tag="PLU11_04165"
     CDS             10716..11441
                     /locus_tag="PLU11_04165"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPN58340.1"
                     /translation="MVTLCFDFGNTRKKVAVFHGPEMKEAVMLPDDSVATIQSLIDRF
                     KPAKSILSSVIDHNPDIETLLAVHTKFHKLSHLTKVAFTTPVGKPETIGADRLALTAA
                     AIHYYPTHNNLVIGMGSCITYNFINKYHEFVGGAISPGLEMRLKSLNYYTAKLPLVKA
                     DSNVPLMGYDTVTNILSGVVLGMAKEMDGFIAAYKEKYRNINVLLTGGDIVYLASHLK
                     NRIFADPDLIFKGLYATSEVNNP"
     gene            11413..12831
                     /locus_tag="PLU11_04170"
     CDS             11413..12831
                     /locus_tag="PLU11_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58341.1"
                     /translation="MQRAKLITPKIIKALRGSQWSFVVLFLAAASPAFSQDNSPYSRY
                     GIGDLVPATNIINRSMGGVTAGYFDQLSINFNNPASYSTFQAIRERKAKKLSSGRAIL
                     DLGLNFENRTLREPNNATKFTASNALFSYVQVGVPLKADWGLSFGIRPVSRISYKILT
                     NEKLIDPITGLPIEDAATLYTGDGGSYLISAGTGFSLFKKEKHGLMEGLSVGFNGGYL
                     FGEKDYSSRRALLSDTVSYYKANYETRTNYGSLYYNMGMQYKLPISKTMQLTIGAFGN
                     FSQKLKGKQDRIRETYVYDDNTGNVRLDSVSETRDVKGTINLPSSMTLGFMLQKFAVQ
                     NKEGGWMLGMDFATQKWSDYRFYGQADSLRNKWELRTGVQINPVPRRNYFSNISYRFG
                     FFFGPDYVKVGQKLPQVGASFGLGLPMAISRQAPNQATFINMAFEVIKRGNDKNLLKE
                     NLFRFSLGFSLSDIWFIKRKYE"
     gene            12863..13438
                     /gene="lptC"
                     /locus_tag="PLU11_04175"
     CDS             12863..13438
                     /gene="lptC"
                     /locus_tag="PLU11_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPN58342.1"
                     /translation="MFTTRHYKITLTALLITGCFFMTGCENDDKVLNDWTRRVVMTEE
                     ATNVVSYMSQDGQLRAKLTAPLMIRTNGDTITVEFPKSLHCDFYNDSTKKVETFLDCR
                     YGIYYENLNKVYLRDSVVVISVNGDTLRTPDLWWDQSTKMFYTDKYANYNGPNKIVRG
                     GTGMEATQDLRRITFKEATGNLKVSENGMPK"
     gene            13647..>13783
                     /locus_tag="PLU11_04180"
     CDS             13647..>13783
                     /locus_tag="PLU11_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN58343.1"
                     /translation="MSFLQRTALITGATRGIGKAMALRLAKEGANIVIAAKSVEENAK
                     LG"
BASE COUNT         3736 a         3148 c         3266 g         3633 t
ORIGIN      
        1 agaaataacg gggtgctgat ctccaaattc caggataaga ccacgggtta ttccattgat
       61 aaattacagg atcgtggtac tttctttgta gatccgggag aagaggttta ttgcggacag
      121 atcattgcgg aaaacattaa gcccggtgac ctggtggtga atgccacgga aggaaagaaa
      181 ctgaccaacc accgtgccag cggaagcgac gatgctaccc gtatcgcgcc taaaacgcag
      241 atgacactgg aagaatgtat ggagtatata cagcaggatg agtgtatcga ggtaacgccc
      301 aactttatcc ggatgcgtaa agtcatcctg aatgaagacg atcgtaaaaa agtgcagaag
      361 agaatggaag cagatgcagt gtaagctgtg aaatgatata gagaggctgt cctaaagggc
      421 agcctttttt ttggccacga aagaccacga aaaggcacga aaaaaaatac aagccacgga
      481 taacacggat ttgcacggat ggccccctaa atcccccgaa gggggacttc agactccgat
      541 agattgcaaa tactttttat caatgctttg cgtatttgta tcccgcaaag cttaaaagac
      601 ttttccttaa acggagtgtc ttataaatct tagcgggaaa tttttacgca agactaaaag
      661 aaagaaatgt ttaaagcttt tgggtcttcg aagtccccct ttgggggacc tgcctatcgg
      721 acaggcaggt ttagggggcc attataccct cttcccggta tagacaaaat ggccccggca
      781 tactacattt gataaaatac tttttatgcc attccgtata ctctttcttt ttctccttgg
      841 cttttcctgc acagccatac aagcccaatg cctttccggc aactgcaccg atggctttgg
      901 taagtttaaa tataaaaacg ataacatata tgaaggcaat ttcaaaggcg gctattgtaa
      961 cggtaagggt aaaatgtttt attcaaacgg ggaattctat gaaggcgact gggtcaatgg
     1021 cacctggaac gggaaggggc tttatacctg gaaggacgga cgaagatttg aaggtcaggt
     1081 aaaggatggt tattttgatg ggtttgggat ttatttcatg acagacagta accggtatga
     1141 aggcaactgg aaagtgggca gttttgaggg taccgggaaa tactttttta aagacggcag
     1201 gtattatgat ggtggctggt tgaaaggcaa gaaatcgggg aaagggaaat ttacctggcc
     1261 tgatggggga agttatatcg gcgattggaa agaagataag tttgagggag aaggcaaacg
     1321 catttataac gacagcagtg aatatgaagg aagctgggta gccagtgtgc aacagggtaa
     1381 agggcggtat gtatacaata acggggatgt atatgcgggg gaatttaaag aaggaaaaag
     1441 aagcgggttg ggtaaatatg tggtgaaagc agatggtcag gtttatgaag gaacttatct
     1501 gaacgggcag cccgagggga agggtaaact ggtgatgggg aataaggatc gttatgatgg
     1561 ggacttcaaa aacggattgg ccgatggcta tggtatcggg gagtatacga ccggaaagcg
     1621 atatgaaggc gaatggaaag aaggaaactg gaacggtaaa gggaaagcta cctggaaaga
     1681 tagttcaagt tatgagggac aatgggtgaa aggaaaaatg cagggaacgg gcactaaaat
     1741 ttacaaaggc ggggaccgct ataccggcaa ctggtttgaa gataagatag aagggcaggg
     1801 cgagtatacc tggaaaaacg gggatgtata taccggtcat ttcaccaatg aaaaaatgtc
     1861 gggacaagga aaaaaagtgt acaaggatgg ctccgtctat gaaggatcct ggctgtttgg
     1921 attatatagc gggaaaggtg tactgagttg gccaaacaag aaccgatatg agggcaattt
     1981 ctcaactggg gtggctttgg ggaatggggc tttttaccga aacggggtgc tgttgtttga
     2041 aggcaatttc aacggaacca gtacgtatag tactgccact acctggcttt taacagatgg
     2101 ccgctttgcc cggcgtacca cttatactga tggttccatc tttgagattt ggcatccgga
     2161 tcagtcgatg tttatcggca gcatcatcga cataggttat aatggatcca ttagtgaaat
     2221 caagccacgt gaaggctcct tgttgtacgc aacgggtaag gtgattagcg gaaaatggga
     2281 agacggaacg attaagcaac ccacatatca ataccagatg aaaatgtcgg aggcggtccc
     2341 tgagtatgta aaaggccgga tctattatgc gtattcaaaa tatgcggatg ccttgaaatc
     2401 attcaataaa gccatcggcg gaaacctgaa agaggatagt gtgtatttat tcaggggtgc
     2461 ggcatattat ggcctgaaat tatatgattc tgcccgtgcg gatctgacca cactattaaa
     2521 gaaacaggct aaaaacaaag aagggattac ctggagggcc aaaaccttac tgtcgctgaa
     2581 agacactaca gccgctttag ctgatttttc aacctatatt aagaatttcc ccggcgactc
     2641 caccggctac tggcagcggg gggttattta tcataatgta aagaattatg atgcagcggt
     2701 taaggattat accacatacc tgcaacgcac taaagcccgt aacgaaaacg tatattatta
     2761 caggggaatc tgttacgaac atcttggtaa gaaaacagaa gcctgtgcgg atttcgaaac
     2821 agccaaaaaa ggaggaaata agaatgcgga tgcccagatc aaaaaacttt gtggcaatcc
     2881 cggctaagca aaaaagaagg gtttttccga acttccggac cgccatttcg ttgtattttt
     2941 gctcctcagt tacaacgtat gaagaaaagc ctgataattg gtttagtgat agtggccgga
     3001 ctcctgctgg caggtccgga agcagttgca caatgttcga tctgtaccaa gaccgcccag
     3061 caactgggtg aaggtccggc caaagggatg aatacaggta tcctgtacct ggcttttgcc
     3121 ccgatggcca ttattggttt tctcgcctat cgctggataa aaaataaccg ccaggcctga
     3181 tcagcggatc tgtaattttt ttatttcccc atccatattc agtaaataaa ttcctgctgg
     3241 caggacaggt aataccaggc ggtaacgggt agaccgtact tcttcctgta acagggttct
     3301 gcctgaaaga tcactgagtt ttaaaaccgc cgttgccgcc tgtttccgga gaataaataa
     3361 ttcggtattc gccggattcg gataaaaggt aaaggccgtt gcgggtttta tgtgcaccgc
     3421 tttgatcgcg gataaaacag cagactgatc tttccggatc atccggacac ggtaataatg
     3481 atgacctgtt attgtaagag gatcggtcaa ctggtatact gactgcccat caatcgcttg
     3541 tttttcgccg atgaattccc agttgccatt ggttgttttt cgttcaattc cgaaatggct
     3601 gatgagttcc ggatcagtta cttcccattt cagctgtaca ttttcccgga ctatagttgc
     3661 gttgaatgtg ataaaatcag cgggtaaaat taatgaacaa tagctggccg ggctgaatga
     3721 tcgggtgaaa gtataatcag ggattacctg tccggccgtt ccggctgcag ccgactgtgg
     3781 caccgggttg caggatacat cacgtccgga ttctacaccg gccgcaccgc cggttacact
     3841 ggtggttatg gctggcaaac ttccggtaaa ataaataatg ccgccactgc caccacctcc
     3901 accaccaaag caacggccga tattgccccc gttatttgaa ttacccccat tgcctccatt
     3961 ggcctcaatg gtaaccggac ctgtatagtt actgatgtgc aggataatgg ttccgccggc
     4021 cccgccacca ccggccccat cggataagga agatccaccc gacccaccgg aagcattaat
     4081 ggactggctg ttgccaagca ggttattcgc ccagataaaa ataataccac ccccattggc
     4141 gcccgataca ttggacaacc cattattaga atgaccagca ccacccccac cgccggcata
     4201 aatttttaat ccgctattac tgctcagact cctgcctgct tcccctttta aggtggcagt
     4261 acaggccacc ccgctgctgg aattaccacc cccggttccg cctgcattca gattggcacc
     4321 cccaccccca ctgttattat gattattacc gccacccccg ccattggccg gtgcaccgcg
     4381 tccgcccgtc tggttactgg ctacatccgc aatcccttct cctttggcag ccccgctttg
     4441 aggactcagg ttagatgcat tgtacgcgta cccgctggca ggtaaggcat cactacaagt
     4501 gccattgctc tgttggaagt taccgccgcg gtaaccactg gcacctgcat aaaccggtgc
     4561 atttaagatc aggtcctgtt ccgtatagat cgcgatcacc ccgccggttc cggtgctgtt
     4621 attccagggt gttgctttaa cagtatctgt tacgtttgcg gatacatatc tggcgaaggg
     4681 aaccaattgc actttaccgg tggcaggcgt gtatgtattt tctaatatat gaaaaagaaa
     4741 aacagaatcg ccgatgattg aacagatcgt accggtttca taattgccgg catcattgag
     4801 ggccgtcacc gttccaaaac tgctgttatt ggtagtagtg atcgtggccc ctttcatttg
     4861 tattaacaga atgggatcat tgacggttag tcccgccgta ctgctgagac ggatacaggc
     4921 tttggcggga ataatttcaa gtacacggta atagctgttg accactccgc tgatagcggt
     4981 ggtttgtccg gatgcaggga tcaggaccga ttgaaagtga aaccataaaa cgaaaacaaa
     5041 gaaaacgcgg atggaccggt tcctgttttt catatgatgg gacgttggtg tcgaagttga
     5101 tacatcgctt atttgcccgc gatatattgg tacaggttga aatttccttg ctccaaagcc
     5161 gtttcaattt ccgctttcag cctgcttttt gaaatatcct tcattctttt ctgcccgatc
     5221 aggtaatacg ctttttcggc aagcagccag cttgtttttt cattagagcc ggatgaaacc
     5281 agtaactctt caattttttc cgcgaactca ttgatccctt ccttcctggc cgcaaccgtt
     5341 ttgatcacgg gcacttctgc tgttttccgg ctgaaagaag gagccagcat caggcgcaga
     5401 ttgcggacaa acatatcggc atccggacga tccgctttat tgaccacaaa gagatcggct
     5461 atttccatca gcccggcctt catggtttga atttcatccc cggcctcggg tactaccact
     5521 acagcagtaa cgtcggccag tccggcaatc tccacttcac tttgtccaac ccccacagtt
     5581 tccacaatga tatagtcaaa acgaaaggct ttcaacaggt ctgttatctc aatgatcttc
     5641 gggtgcaacc cacccatgga acccctggtg gccagcgaac ggataaaaac attgtcgttg
     5701 gtataccaat cgctcatccg tatccggtct cccaataccg cccccaggtt aaagggagag
     5761 gaggggtcta cacaaactac ggcaactttc cggcctttcc caacgaatac cccgattaat
     5821 gcgtctacca gggtgctttt acctgcaccg ggcgggccgg ttataccgat aattttcgaa
     5881 tgggagggtg ggagagactg cagtaatttt tcatagcccg ggtgttcgtt ttccaccaaa
     5941 gtaatactcc gggccaatgc tttgctgttt ccctgctgaa cctgtgctaa taattcctgc
     6001 cacataattt ttgaatgcta taactgcaaa agtaaacaga acctgtttcc cctttatttt
     6061 ttgccttcag cggcattgcg ctgcaacagg taaagtttac tgtgtttgag aaaagtgaga
     6121 tgcgccacat tccgggcaat cacgtaacca taaaatccat cccggaaacc tccccgcaga
     6181 atgaacccgg aaaaaaaagc ccagcagggg ttaaaaaaaa ttttgaacag gctagtcttt
     6241 ttgccacggt tgaaatagct ttcggcggag atggtgctga atttattatt ctgtgtgata
     6301 tgttcttcaa tcgagtagta agaatagtgc aggatatctc ccgtaagatg aacggtgggt
     6361 tgcggcgatt caaactccac tttatcatgc gggttggtgc cgccccatat ggccagttgc
     6421 ttattaaata aacgcaattt acgatcagga taccaggcac catgccggat aaaccggcca
     6481 caataatatg tacaacggtt catggtatag ccggcggccg ggttattgtt tttggcggag
     6541 agtatggatt gttgtaaacg ttcatccagg gcctcgtccg catccaggct gagtaccagg
     6601 tcatattcag ccagggccag ggccttattt ttttgctcaa tataacccgc aaatgcctgt
     6661 tgtaccaccc gggccccttt tgcaacggca atggcacagg tattgtcagt agaatgggag
     6721 tcgagcacaa tcacatcatc ggcaacagac agtactgaat caatacagcg gccgatattg
     6781 cgctcttcgt tgaaggtgat aatcgcaacc gataacttat tcatctaccc aaaaaatttt
     6841 catatataat atcgggtata tttgtaaaac ccattaaact attgaggatt gaagatacct
     6901 aatcaaacga tagcatttct tgaaaaatat ttcgatcgca tatttgtcat taccctggaa
     6961 agagccggcg accgccaggt aaaagtgaaa gaacggctgg aaggattgac ttttgaattt
     7021 ttttacggcg tggacaaaca cgggctcagc tgggagaaag taaaccagga aggtgtctat
     7081 gacgataaaa aagcccggca actgaaccgt tacagtaaag ggatggtgct gggacatatt
     7141 gcctgtgcgc tttctcaccg taaattatat cagcatattg tggaaaaagg ttatcagcgg
     7201 gtactcatat ttgaagatga tgccgtgcct ttgtttgaca aggatggcga attacaacaa
     7261 agtgtggagg aattgcctgc aaactgggaa gtgatttatt tcggctataa taaaaatgaa
     7321 acggctaccc cggccctaaa aagaaagcag gctttttacc gggtcatcag tcacctgaag
     7381 ctgattaaat ggtcgcccct catggtcagt aacctgttgc caaagcctta ttccccccat
     7441 ctgcagaaag cgggcttcca tgatctgacc cattcgtatg ccgttaccag ggaggcttgc
     7501 ctgaaactga tccgggcaca aacaccggta gtctttaatg cagatccgtt ggtttcacac
     7561 ctgattatga atggggaatt aagtgctttt ataacccgga aaaaattctt tacacaggag
     7621 cagtttttag atccggcaca tcgttccttt atccatcact gatcagatga tccggtaatt
     7681 gcggaaactg aaggggcgaa taccggtttt cttctcaaac cagtacagca gtcttttttt
     7741 cagggagaat ttcttttggg tgatatccag ttgtaccgcc ggattgccct tgctcaccct
     7801 ttctttcatc acggcagggt gcttgcccag aaattttttg agtgaatcaa agtcattgta
     7861 gtcaaaaaag tcctctgttt tcaggaactc ctggaagccg gcctcttctc catgccagaa
     7921 tacacccgta ttcttaagct tccgcttcat cagttccggg ctcttcaccc agccataatg
     7981 aaagacccag gccggtattt gtttaacatt cagccgggta ttgcctttac ggaacccctg
     8041 ggcatcccgg aaagccgtaa tcgttttatc gttccggatg atccgggcct ccttggtata
     8101 ccatttacgg ctatcgccca cgtaatcgta agtgccgtaa aaatggagat agtggaacaa
     8161 gagcccttcg acttttttat tatccttata catcagggcc gcatcccgga ttgctgcctg
     8221 gtattgttct ggaattactt cgtcggcctg aatataaaac agccagtccg aatccggcgc
     8281 acaggccgct ttggccttat cggtttccac ggccagcact ttcccgcctt ttttcagctc
     8341 cgggtcccag accgaatgaa taattttaat tttagggttc ccgatggata gaatcagggc
     8401 ttcggtttca tcctcactat ccccgatact gacgatcatt tcatccacca ccggtaaaac
     8461 ggagagaatc gcttccacta ccgggtaatc attgagcacg gcatttctga taatcgtaaa
     8521 tcctgacatc ttcatggcgc aaaaatagtc tttctgcatc ggcttcccga aacaaaccca
     8581 ttgcgaaaaa aaatgtaact tccttattaa ttgctatgaa gcctgacgca ttgctttcac
     8641 cccgctatct ttctttagta ctggccgccc tgttggttac cggcctctta ttttcccgtg
     8701 ccttgctcag tattatgatg gcaagtggtc tgctcctgct cctctttcct tctgtaagac
     8761 aggttttcgg tgctgtattc aaacggccct tacttttcct gctggcgggt attattatct
     8821 tctatgcctg gctgttcttc ctggatccgc aacaatacac ccttgaaaga atgggcctgc
     8881 accttggtgt aatcagttta ctctgtctcc ttcattttat ggccgccact tcagactggc
     8941 tgctgccatt tattttatcc gtgggtgtat tgagtacctt tcccagcgtg tatgatatgg
     9001 tgatgcacga gggactggcc gcattatatg aaaaaggaca agtggcgtat acactgatgc
     9061 agggagatca ccagcgattt accatctgga tcagtggttg cctcgccttt tcctggtatt
     9121 actggctgat ggaacataaa aagcaggcat tttatttcat cctttatttc accattttcc
     9181 tgttattact atctgtgcgt acgggttggt tgtttgccgg actggttacc gtggctggtg
     9241 ggcttatcta tttacaaaga cagcacaaaa gagtttactg gatcagggcg atactgggtt
     9301 taatactcct gttggccatt gcatatagtg tcccttttgt acgaaataag atccaatata
     9361 tgcaatggga gtggcgggaa aagaaagcag cggaacagct cggcagttcg gatgccatcc
     9421 gctggatggt gaatgaatcg gcggtggaac tcatccgcga aaatcccaaa ggattgggtg
     9481 aggcaaaagc cgcgctggca ttgaaagaac ggataaagat caactaccct gaatcatcgg
     9541 taagttttca atggccgttt aaccagtacc tcaaatggtg gctcactgcg ggctgggtga
     9601 tggggtccct cccgttattg ctggtgcttt acctgttata ccggctgtac tttacccgca
     9661 attacctgac cgccgtatgg gttttattta ttgccctcac cggcctgtac gaaagcaccc
     9721 tcgaaatgca gtatggattt tttctcggcg tatttttcac cgggctgctt tatttatatg
     9781 aaagcgggtt aagtaaacgt acggttttaa gagcataccg ataggtttcc cgcagatagt
     9841 gcagattaat acacgcagat aaccgttgat tatatcacgg agatactgtg gtacattttt
     9901 tttaggctag tttactttta acccaattat gtattttctg cgcgatctgc gtgtattgat
     9961 ctgcgaaaat ctgcgggaaa atgtatttga caatctcctt tcaaccgctg cattatcttt
    10021 acatcctcta tgactaattt catccccatt ttcccccttg gcctcgtggt atatcctggt
    10081 gaaagcctta acctgcatat ttttgaaccc cggtacaaac aactgatcca ggaatgccat
    10141 gccgccaaaa aaccctttgg tattccaaca gtgatcaata ataaactgca ggaattcgga
    10201 gccatggtgg agattgatga actgacgaag atctatgaca acggagagat ggatgtcaag
    10261 acaaagggcc tgaggatttt ccgtatcctc gaattaatta aagaaattcc ggacaaactg
    10321 tacagtggcg ctatcgttac ctacccggac aattacggcc agggcaatcc ggataccatg
    10381 cgcaaactga tgaccagtat ccgggaacta cacggacttt taaaactcag caaagacttt
    10441 aaaaagaccg acgaggaaat gcttagttat gatgtggcac atcatatcgg cctctccctc
    10501 gatgaagaat acgaactgat gggactcatg gacgaaaaac aacggatagc gtatatcaag
    10561 cggcatttaa tcaaagtgat ccccatggta tccggtctgg aacaactcaa agagaagatc
    10621 aaactgaacg gacattttaa aaacctgagc gggtttgata tacaataatt ggcacatgct
    10681 gtatttccct acttttaccc ctaattttca gttatatggt tacgctctgt tttgattttg
    10741 gtaatacccg gaaaaaagtg gctgtatttc acggtccgga aatgaaagaa gccgttatgt
    10801 tgccagatga ttcagtggct acgattcaat cgctgattga ccggtttaaa cccgccaaat
    10861 ccattctttc ttctgtgatc gatcataatc cggatattga gaccttactg gcggtgcata
    10921 ccaaattcca taaactgagt cacctgacca aagtggcttt taccacaccg gtaggtaagc
    10981 ccgaaaccat cggggcggac cggttggcac tgactgcagc cgccattcat tattacccaa
    11041 cccataataa cctggtcatc ggaatgggct cctgcattac ctacaatttt atcaataaat
    11101 accatgagtt tgtgggcgga gctatctctc ccggactcga aatgcggctg aaatcgctta
    11161 attactatac ggcgaagctg cccctggtga aggccgatag caatgtaccc ctgatgggct
    11221 acgacacggt cactaatatc ctctccgggg tggtgctggg aatggctaag gaaatggatg
    11281 gatttattgc cgcttacaag gagaaatacc ggaacattaa cgtgctttta accgggggag
    11341 atatagtgta tttggcttca caccttaaaa acaggatatt tgcagaccct gatttaatat
    11401 ttaaaggtct gtatgcaacg agcgaagtta ataaccccta agataatcaa ggcgttacga
    11461 ggttcccaat ggagttttgt ggtgcttttt ctggcagctg ctagccctgc attttcgcag
    11521 gacaactccc cttattcccg ttatgggatc ggagatctgg ttccggccac aaatattatt
    11581 aaccggagta tgggtggagt aaccgccggt tatttcgacc agctttccat caattttaat
    11641 aacccggcct cctattcgac cttccaggct atccgggaac ggaaagcaaa aaaactcagt
    11701 tccggccggg ccatcctgga cctcggactg aacttcgaaa accgcacatt acgcgaaccc
    11761 aataatgcca ccaagtttac agccagcaat gcgctgtttt cctatgtaca ggtgggggtg
    11821 cccctgaaag cggattgggg attgagcttt ggtatccgcc cggtttcccg gatcagttat
    11881 aagattctga ccaacgaaaa gctgattgac ccgattaccg gtttgcccat cgaagatgca
    11941 gcgacattgt ataccggtga tggaggttcc tacctgattt ctgcaggaac cggtttctcc
    12001 ctttttaaga aagagaagca tggtctgatg gaaggattga gtgttggctt taatgggggg
    12061 tatttatttg gtgaaaaaga ttacagttcc cgccgtgccc tgctcagcga tacagtgtct
    12121 tattacaaag ccaattatga aacccgaaca aattatggca gcctttatta taatatgggg
    12181 atgcaatata aattaccgat cagcaagacc atgcagctga ccatcggggc ttttggaaat
    12241 ttctcccaga agctgaaagg taagcaagac cgcatccgcg agacgtatgt atatgatgat
    12301 aataccggga atgtccgtct cgatagtgta tcggaaaccc gcgatgtgaa aggaaccatt
    12361 aacctgcctt ccagcatgac ccttggattt atgctgcaga agtttgcggt acagaacaaa
    12421 gaagggggat ggatgctggg aatggatttt gccacacaga aatggagtga ttaccgtttt
    12481 tacggacagg ccgattccct gcgtaacaaa tgggaactcc gtaccggtgt acagatcaac
    12541 cccgtgcccc gccgtaatta tttcagcaat atctcctatc gtttcgggtt tttcttcgga
    12601 ccggattatg taaaagtggg acaaaagctg ccccaggtag gagccagttt cggactgggt
    12661 ctgccaatgg cgatcagtcg tcaggcaccc aaccaggcta cttttatcaa catggctttt
    12721 gaagtgatta aaagaggaaa tgataagaac ctgctgaaag aaaacctgtt ccgtttttca
    12781 ctgggcttct cactcagcga tatctggttt atcaaacgta aatacgaata accggtttcc
    12841 cgtttttgat cattcattga aaatgttcac gacacgccat tataaaatca ctttaacagc
    12901 cttgttgatc acaggctgtt tttttatgac cggctgtgag aatgatgata aagtacttaa
    12961 cgactggacc cgccgggtgg tcatgaccga ggaagccacc aatgtggtca gttatatgag
    13021 ccaggatggg caactgcggg ccaaactcac ggcgccctta atgatccgta ccaacggaga
    13081 taccatcacg gttgaatttc ccaaaagcct gcactgtgat ttctataatg acagtacgaa
    13141 gaaagtggaa accttcctgg attgccgcta tggtatttat tatgaaaacc tcaacaaagt
    13201 ctatctccgc gacagcgtag tggtgatcag tgtaaacggc gataccctcc gcacccccga
    13261 tctctggtgg gaccaatcga ccaaaatgtt ttatacagat aagtacgcga actataacgg
    13321 tcccaataaa atcgtgcgtg gaggaaccgg tatggaagcc acccaggacc tgcgccgtat
    13381 tacgttcaaa gaagccaccg ggaatttgaa agtttccgaa aatggaatgc ccaagtaaaa
    13441 gttgtcagtt gtcagatgtc agttgtcagg agttttctta gttgtttttt caaaatattg
    13501 gtctcttcca acctccaacc tcgaacctcg aacctccaac ctcgaacttc gaacttcgaa
    13561 cctccaacgc tcgacccttt tctaataaaa catttgtcaa cccaattttc taagataact
    13621 ttatctaaac cctcaaccct tttaatatgt cattcttaca acgcactgcc ctcattaccg
    13681 gagccacccg tggtatcggg aaagccatgg ctcttcgatt agccaaagaa ggagccaata
    13741 ttgtgattgc cgccaagagt gtggaagaga atgccaaact ggg
//
LOCUS       DAOPMW010000047        13732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4533_length_13732_cov_9.104043, whole genome shotgun sequence.
ACCESSION   DAOPMW010000047 DAOPMW010000000
VERSION     DAOPMW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_13732_cov_9.104043"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLU11_04185"
     CDS             <1..654
                     /locus_tag="PLU11_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN58344.1"
                     /translation="DLKAMEIKRTKLDLALFYEDDFKEVDAVIRTRLSRKNDKGIVLL
                     HGLPGTGKTTYLRYLVGKIRKRVLFLSPSVAGNLMNPDFIELLVDNPNTVLVIEDAEN
                     IIMDRRHNSSSAVSNLLNISDGLLADFLNVQLICTFNSALTTMDNALLRKGRLIARYE
                     FGKLGVSKSQRLSKHLGFDQAITEPMTIAEIANPHEKNNGEKPVEVIGFRLAETMMN"
     gene            1374..2489
                     /locus_tag="PLU11_04190"
     CDS             1374..2489
                     /locus_tag="PLU11_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPN58345.1"
                     /translation="MKKVKLNAWQVALVFKNGAYLRCLTEGTYWFWANENVYVYDMTQ
                     SFVAPVELNILLLDEKLSGLLEVVEVKEEELVLQLENGLLKTILTAGRYTFWKSVIRY
                     AFIRVDISKPEIDESINKSWLNKSMVMAWVRTFEVLNYEKGLLYIDGKFREVLEPGTY
                     YWWKNRVSVQVYRADMRQLQLEINGQEILTRDKAALRINAWAQYKLADIQKALVLNKE
                     YDKQLYVAFQLSLREYVAGYTFDELLEKKDSLAPFVLEQVKEKATGLGVEVLNFGIRD
                     IILPGDVKEIMNQVLIADKKAQANSIMRREETASTRSLLNTAKLMEDNAMLWKLKEME
                     YVENIAEKISSISVSGNGMLLDQLKQLFVSGTEVKKE"
     gene            2574..4025
                     /locus_tag="PLU11_04195"
     CDS             2574..4025
                     /locus_tag="PLU11_04195"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPN58346.1"
                     /translation="MSKLKLSGKQIRAIGYPEGPVISVAMQVMNSKFKHSTEKEALEI
                     LKQVLNAPENYLEDEQLKTVAEKLMPAPLTEGAEISLNQTGIAFSIFGQEHIEEGAMH
                     QMYQAAKLPVSVAGALMPDAHSGYGLPIGGVLATDNAVIPYGVGVDIGCRMCLSVFDI
                     APKELVDRESFFTRELGEATLFGSGGQFDHAENHAVMEHELFYQLPLLKHLHGRAWKQ
                     LGSSGSGNHFAEFGIVEILEKDPVLGLEPGSYTGFLTHSGSRALGATIANHYTKLAIS
                     KRHLPQEAKNLAWLDMNEEEGIEYWMAMNLAGDYASACHHIIHDKIAKQLGRKPMKRV
                     ENHHNFAWKEKWEGRDVIVHRKGATPAGKDVLGIIPGSMTADGFIVKGKGEMAAVNSA
                     SHGAGRKMSRTRAIASVTDKQFKDELKKFGVKLLGGGLDESPFAYKDIHTVMQSQKAL
                     VDVVGRFTPKIVKMDGAKHRSWGKKGQSEIAGE"
     gene            complement(4113..4523)
                     /locus_tag="PLU11_04200"
     CDS             complement(4113..4523)
                     /locus_tag="PLU11_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPN58347.1"
                     /translation="MKQLTVLTALFIVFTSFRSDTTVKVDLYNSKWQLKTIHSKEGAA
                     YIYTNAFIRFDETQKRVSGNGSCNSFGGSVTINDNSLQIGNLFSTKMYCEAVQPIETD
                     FFAALGKVTRYEIKGNSLLMYQGDTLLLEFEVQQ"
     gene            complement(5421..6665)
                     /locus_tag="PLU11_04205"
     CDS             complement(5421..6665)
                     /locus_tag="PLU11_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPN58348.1"
                     /translation="MNQRFYSLDVFRGATVCLMILVNNPGSWGSIYAPLEHAPWHGLT
                     PTDLVFPFFLFAVGNAMSFVMPRLEAGGDGLFWKKVLKRTALIFLIGLFLNWWPFVTE
                     VNRLVEGTTDFQKETIFKGFTWIDYYKDKAGTIIESVKGVRVLGVLQRIALAYFFASV
                     LVYYLKARKAFLAGLILLLVYWVLCYAGNPADPYSLKGWFGTAIDKWFLGQAHMYKGE
                     GIPFDPEGLMSTLPAIVQVIFGYLVGDYIQKKSKVLPLPDQSGATTGGMYEMLTGIFV
                     IGIAMLVTGYCWDMVFPINKKIWTSSYVIYTTGLAIMTIATMIYMIEIKKVRGGLARF
                     FDVFGKNALFVFALSAFLPKGLALIKLGDGVNPWNWLYKKVLIHTPGDKELGSLLYAL
                     CVITFMWAICWWMDKKKIYVKV"
     gene            7006..7506
                     /locus_tag="PLU11_04210"
     CDS             7006..7506
                     /locus_tag="PLU11_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPN58349.1"
                     /translation="MSKKWLAIYTRPRWEKKVNQLLTEKGFESYCPLNKVKRKWSDRI
                     KVVEEPLFKSYVFVKVSEDDRTEVRMTTGAINYVYWNGKPAVIKEKEISAIRRFLNEH
                     EYVEARPMDAKVNQRVRIIKGTFMDQEGKILELRHKTAKIAIDSLGYILVAYIDRTKL
                     TSALPE"
     gene            7560..8336
                     /locus_tag="PLU11_04215"
     CDS             7560..8336
                     /locus_tag="PLU11_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPN58350.1"
                     /translation="MKFTRFLLLLALPLYFISCKPLQKIPNYLENVNDSTGKGVVKVV
                     ELKIQKNDLLSIQIFSLSTQPDKADILFNQPTLGAAGGAPGYLVDNNGNIEHHRLGVI
                     HAEGLTKQQLADEVKKRLKEPVEVLADPTVLVRFMNLKVTVLGMVGQEGPISVPGEKL
                     NIFEAVGLAGGINDFGKKNTVKVIRETDGQREVGFLDLTSQKVFESPYYNLVQNDLVI
                     VEATSQKQTEQQQTKIMQKITFAFTIVTAVAALSNIFIRN"
     gene            8364..10793
                     /locus_tag="PLU11_04220"
     CDS             8364..10793
                     /locus_tag="PLU11_04220"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPN58351.1"
                     /translation="MEETHINPNYNPNKSDLSSLSFRDLFYKYIRFLPVFILSVALML
                     LGAYLYLRWTVPVYSASGSMIIKDEGKNRGAGDKVEELFSSNKAQNIQSEIEILKSRP
                     LMQRVVMKLNLQFSYYAIGKVKTINVYNQGPFLIRALNITDSSQPFIFKIRFVNDTDF
                     RINEEATVYHFGQEIINRYGSFVFIKNVGLPANNIYSVQWVPTFQAAGGYASTLQIMP
                     KTIGTGILNISMRCPNPHLGSAILNGLMEEYGAYSLEQKKLSSDQILAFIEGRLVDIG
                     TKLDSVQRMYLDYQTRYNMIDADVQSSSYFGIINESDKTINEQNYRISVAEKIDEYLS
                     DKKNAFAKIVVPSTLGLDDPTLNDLVNLYNKVQLERKQLVDGNVPAGHPLVKDADNQV
                     EKIRLSIRENIRNINKASADLVSDVRKRSNLGEEQLRAMPVKIKELAEIKRQVETYQD
                     LFKLFTQKKEETAISRASNTPNSEIIDRSYPQVSPVKPDKRSIQAMAILLGLAIPALF
                     IFVSEVLNDKVTTRFDIEKITQAPILGEIGHSYSESTLVVSKTTRSMVAEQFRIIRSN
                     LQYVLNKKDKAVIITTSSFSGEGKSYVSTNMGAVLALAGKRTVILEFDIRKPKVLSGL
                     GMSKGPGITNYLVGKMTNLEELIRPVPEHENLFVLGCGPVPPNPSEMLLDPRLDDMFN
                     WLKNHFDIVIIDTAPVGMVSDAMTLSKFADCTLYLVRQSHTFKKQIALIDEFYIANKL
                     PKVSIIINDVKLKPGYGYYGYGRYGYGYGYGYGSYYEEETAPDNFFERILAKLDIRRL
                     LGLKKKKKKNK"
     gene            10795..11754
                     /locus_tag="PLU11_04225"
     CDS             10795..11754
                     /locus_tag="PLU11_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN58352.1"
                     /translation="MMRVLVTGGAGFIGSNLVEALLKDERVSFVRVLDNLSTGFLKNI
                     EPYMTDPRFEFLEGDIRDFDTCKKAVEGIDVITHQAALGSVPRSIYDPLTTNGVNITG
                     ALNVFTAAKEAGIKRVVYAASSSTYGDHPGLPKVEDIIGNPLSPYAVTKYVNELYARV
                     YASLYQMEFVGLRYFNIFGPRQNPNGPYAAVIPLFAEALINQKPPTINGDGSHSRDFT
                     FVANAVQANILSLFSKNKEAVNQVYNIACGEQTSLLELFNGLNKEAKTTLQPIFGPER
                     LGDVKHSLADISKAKQLLGYNPLVSVEEGLKVTFGWYKKKFEV"
     gene            11904..12263
                     /locus_tag="PLU11_04230"
     CDS             11904..12263
                     /locus_tag="PLU11_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN58353.1"
                     /translation="MHTYSFEKLEVWQLARKMVVSIYKLTALFPPEEKFGMISQMRRA
                     SVSICSNIAEGSGRKTAKDQAAFYNTSYSSLLELLNQLIIAVDLGWLPAEQLIQIRRE
                     IEILSNKLNSLRNSRLQ"
     gene            12362..13441
                     /gene="rfbB"
                     /locus_tag="PLU11_04235"
     CDS             12362..13441
                     /gene="rfbB"
                     /locus_tag="PLU11_04235"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPN58354.1"
                     /translation="MPSSSTTTTKTIIITGGAGFIGSHVVRLFVTRYPDYKIINLDAL
                     TYAGNLENLRDIENAPNYFFEKADILDKDAIERIFVLHQPDAVIHLAAESHVDRSIIS
                     PLDFVYTNVIGTVNLLNSARHFWKDNFAGKRFYHVSTDEVYGALGDTGFFTEDTKYDP
                     HSPYSASKAASDHFVRAYHDTYGLPVVVSNCSNNYGPNHFPEKLIPLFINNIITKKPL
                     PVYGDGLYTRDWLFVKDHATAIDNVFHSGQNGQTYNIGGFNEWRNIDLVKLLCKQMDE
                     KLGNAPGTSEVLITYVKDRPGHDRRYAIDATKINKELGWSPSVTFEEGLSQTIDWYLH
                     NTEWLKNVTSGAYQHYYDEQYKDRN"
     gene            13546..>13732
                     /locus_tag="PLU11_04240"
     CDS             13546..>13732
                     /locus_tag="PLU11_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPN58355.1"
                     /translation="MKGIILAGGSGTRLYPITKAISKQLMPIYDKPMIYYPLSVLMMA
                     GIREVLIITTPEDNPSFI"
BASE COUNT         3965 a         3117 c         3251 g         3399 t
ORIGIN      
        1 gacctgaaag ccatggagat taaacggaca aaactggacc tggctctttt ttatgaagat
       61 gactttaaag aagtggatgc ggtgatccgt acaaggctca gccggaagaa tgataaaggg
      121 attgtcctgt tgcacggact ccccggtacg ggtaagacga cttatctccg gtacctggta
      181 ggcaaaatcc ggaagcgggt cctctttctg tcgccttcag tagccggaaa cctgatgaat
      241 ccggacttca tcgaattatt ggtggacaac ccgaatacgg tgctggtgat tgaagacgcg
      301 gaaaatatta tcatggaccg acggcacaac agcagttccg ctgtgtccaa cctgctgaat
      361 atttcagatg ggttgctggc cgacttccta aatgtgcagc tgatctgcac gttcaatagt
      421 gcgctgacca cgatggacaa cgcgctgtta cggaaaggaa ggctgatcgc ccggtatgag
      481 tttggaaaac tcggcgtcag taaatcacag cggttgagta aacacctggg atttgaccaa
      541 gcgatcactg agcccatgac aattgctgaa attgccaatc cgcatgaaaa gaataatggg
      601 gagaaacccg tagaagtgat cggtttccgt ctggcggaga cgatgatgaa ttgattatta
      661 aggtttacgc acttcggatt tcagtatata taaaaatgta gtagagtccg atcagccact
      721 cattactgat tactcattac tcattcattt gagtgatccg acgtgactgt ttgtataaaa
      781 gcttgcttaa ccgcaagatg atgatgtccg tgaatcaacc cgggcggatg tttaaacgca
      841 tcgcagtgca gaggcagtag ctatctgtat gctgttgagt aaacgggtat gcatcattat
      901 tcgcttgtgt gatgggttcg agtcccattc ccgtataagg gatagctcag ttggtagagc
      961 aaagcactta cgcaggttcg aatcctgctc cgtctcaaaa acggatagct cagtggcaga
     1021 gcactacaat cataatgtag cggaatggaa gcaggccact tccttaaaat tggccaaaca
     1081 acgaaggccg gctttgacaa caaaggaatt ttcagatcag gtgattaaac aatcacccct
     1141 tgtgaattcc gggaaagccg gtgaatcgcc tgcaggataa tccatcctgt tgtacaacgg
     1201 ctgagtccgg ggtaatcagt cggatgactc tttatcctca ttcagtcagc acaacagtac
     1261 ggtcagcctc cgcggttcgt gggtcttgcc gatcaccggc cttttatttt gaacattccg
     1321 ttttacgatc atgctaaaca acggaggatc gggcaacgga caaaaaactg tatatgaaaa
     1381 aagtaaaact aaatgcctgg caggtagccc tggtatttaa gaacggcgct tacttgcggt
     1441 gcctgacaga agggacatac tggttctggg ccaatgaaaa tgtgtatgtc tatgatatga
     1501 cacagtcttt cgtagcacct gtggaactga atatcctgtt actggatgaa aagctctcgg
     1561 gtttactcga agtggtggaa gtaaaggaag aagaactggt attgcagctg gaaaatggtt
     1621 tgttgaaaac catactgact gccggccggt acactttctg gaaatcggtg atccggtatg
     1681 catttatccg ggtggatatc agcaagccgg agatagacga atccatcaat aaatcctggt
     1741 tgaacaagtc catggtaatg gcctgggtaa ggacctttga agtgttgaac tatgaaaaag
     1801 gcctgctgta tatcgatggt aaattcaggg aagtgctgga gccgggtaca tattactggt
     1861 ggaagaacag ggtatccgta caggtgtacc gggccgatat gcggcaactg caactggaga
     1921 tcaacgggca ggagatcctg acccgggata aagcggcctt gcggatcaat gcctgggcgc
     1981 agtataaact ggcggatatt cagaaggcat tggtgctgaa taaggaatac gataaacaat
     2041 tgtatgtcgc ttttcagctc tctctccggg agtatgtcgc aggatacact ttcgatgaat
     2101 tgctggagaa aaaagacagc ctggcgccct ttgtcctgga acaggtcaaa gaaaaagcga
     2161 ctggtctggg cgtagaggta ctgaacttcg gtatccggga tatcatcctg ccgggtgatg
     2221 tcaaggagat catgaatcag gtactgatcg cagataaaaa agcacaggcc aactccatca
     2281 tgcggcggga agaaaccgcc agcaccagga gcttactgaa caccgcgaaa ctgatggaag
     2341 acaacgcgat gctctggaaa ctgaaagaga tggaatacgt ggagaatatc gcggaaaaga
     2401 tcagctccat cagtgtatcc ggtaacggta tgttactgga tcagctgaaa cagctctttg
     2461 tttccggtac tgaagtgaaa aaagaatagc aacaacaggt tacctgtcac attccagggc
     2521 aggtaacctt ttaaacaatc tggaaagaac gaattatatt ggagaaagaa atcatgagca
     2581 aactaaaatt atcaggaaaa caaatacgcg ccatcggcta cccggaagga ccggtgatca
     2641 gtgtggccat gcaggtgatg aacagcaagt tcaaacacag cacggagaaa gaagcattgg
     2701 aaatactgaa acaggtattg aatgccccgg agaattacct ggaagatgaa caattgaaaa
     2761 cggtggcaga gaagttaatg ccggccccat tgaccgaagg cgcggagata tcactgaatc
     2821 agaccgggat cgccttcagc attttcggtc aggaacatat tgaggaaggc gccatgcacc
     2881 agatgtacca ggcggccaaa ctcccggtga gtgtggccgg tgccctgatg ccagatgcac
     2941 atagcgggta cggtttaccg atcgggggag tcctggctac ggataatgcc gtgattccgt
     3001 acggagtggg cgtggatatt ggttgccgga tgtgcctgag tgtttttgat attgcaccga
     3061 aagagctggt ggatcgtgaa tccttcttta cccgtgaact gggagaagct actttgtttg
     3121 gaagtggtgg ccagtttgat cacgccgaaa atcatgctgt gatggaacat gaattgtttt
     3181 accagttacc gctgttaaag cacttacatg gacgggcctg gaaacaactg ggaagttcag
     3241 gtagcggaaa ccactttgcc gaattcggga tcgtggagat tctggaaaag gatccggtgc
     3301 tcggactgga gccgggatct tacaccggat tcctgacgca ttccggttcg cgggcgctgg
     3361 gagcgactat tgctaaccat tacacgaaac tggcgatcag taaaaggcat ttaccccagg
     3421 aggcgaaaaa cctggcctgg ctggatatga atgaagaaga aggaatcgaa tactggatgg
     3481 ccatgaacct ggccggggat tatgccagcg cctgccatca tatcatccat gataaaattg
     3541 caaagcaact gggacgtaaa ccgatgaagc gggtggaaaa tcatcacaat tttgcgtgga
     3601 aagaaaaatg ggaaggccgt gatgtgatcg tacaccgtaa aggcgccacc ccggctggta
     3661 aagatgtgtt gggaatcatt ccgggttcta tgaccgccga cggatttatt gtaaaaggga
     3721 aaggggaaat ggctgccgtc aactctgctt cacatggagc aggacgaaaa atgagccgta
     3781 cccgggccat cgcttccgta acggataaac agttcaaaga tgaattgaaa aaattcggag
     3841 tgaaactgct cggtggcgga ttggatgaat cacccttcgc ctacaaggat attcataccg
     3901 tcatgcaatc ccaaaaagcc ctggtggatg tggtgggtcg attcaccccg aaaattgtga
     3961 agatggacgg ggcgaagcac cggagctggg ggaagaaggg tcaatcggaa attgccgggg
     4021 agtgagcagg tgctgaacat tgataaatga acattgaata ttgaacattg gtttacttag
     4081 caacgcaatg ctcaatattc aatgttcaat tatcattgct gaacctcaaa ctccagcaac
     4141 aacgtatccc cctggtacat cagcaaacta ttgcctttaa tttcataccg ggtgacttta
     4201 cccaatgctg cgaagaaatc ggtttcaata ggttgtaccg cttcgcaata cattttggtg
     4261 gagaataaat ttccaatttg caggctgttg tcattgatgg ttactgaacc gccaaaactg
     4321 ttacaacttc cattgccact cacccttttt tgcgtttcat caaaccggat aaaggcattg
     4381 gtgtaaatat aggcggctcc ttctttggaa tggatggtct tcagttgcca tttgctgttg
     4441 tataagtcca ctttaacggt tgtgtctgaa cgaaaagaag taaaaacaat gaataaggcg
     4501 gtcaaaacgg ttagctgttt catatttgtt gattatacaa aacagatgcg ggccgggaag
     4561 ggaatacaca ggtgccggga aacggagtta ttaaaaatat gttaaggctt aaaagatgtc
     4621 aggtgtcagt cggcagttag cagtcgggag tcgggagtca acagcccctc cttagtatgt
     4681 gttttctaaa cattctaaaa tatacccccc tttaacattt cgcccctacg gggctctgtg
     4741 ggatgcttgc ggaattttcc ttctaataat aggccacccc taacggggcg gaatacatgg
     4801 aaatctcctt ataaagatct ataccgtcgt taaacaacct gctatttaca ggtcgcccct
     4861 acggggctct atggactact ggtgaaattt tctttctatt aacaggtcgc ccctagcggg
     4921 gcggaataca atggaaattc tctttctaat aatatgccac ccttaacggg gcggaataca
     4981 atggaaattc tctttctatt aacaggccac ccctaacggg gcggaataca ttggaaattt
     5041 ccttataaag atctaaacct ttgtcaaaca acctgctatt aacatttcgc ccctacaggg
     5101 ctctatggga tgcttgtgga attttctttc tactgatagg acacccctaa cggggcggaa
     5161 tacattggaa atttccttat aaagatctat accttcgtta aacaatctgc tatttacatt
     5221 tcgcccctac atggctctgt gggatgcttg cggaattttc cttctaataa tatgccaccc
     5281 ttaacggggc ggaatacaat ggaaatctac atataaacgt ctaaacaact aaacgctcct
     5341 ataagtttct ttcttcttta gtaccctcta aaccgttctc tcaacctctt tcttctttag
     5401 ttctctctaa accgttctct ctaaaccttc acgtagatct tcttcttatc catccaccag
     5461 caaatcgccc acataaacgt gatcacgcag agtgcataca gtaatgaacc cagttcctta
     5521 tcgccgggag tatgaatcag tactttttta tacaaccagt tccagggatt cacgccatcg
     5581 ccgagtttga tcagggccag tccttttggc aaaaacgcac tgagggcaaa aacgaataag
     5641 gcgtttttgc caaacacatc aaagaaacgg gccagtccgc cacgtacttt ttttatttcg
     5701 atcatataga tcatcgtggc aatagtcatt atcgccagac cagtggtata gatcacatag
     5761 gagctggtcc agattttttt attgatcggg aaaaccatat cccaacagta accggttacg
     5821 agcatcgcaa taccgatcac aaaaatgccg gtgagcattt catacatgcc accggtagtg
     5881 gcaccggact gatcgggcag gggaaggact ttgctttttt tctgaatata gtccccgacg
     5941 agatagccaa aaatcacctg tacaatagca ggcaatgtac tcatcagtcc ttccggatca
     6001 aagggaatgc cttcgccctt gtacatatgg gcttggccca ggaaccactt atcgatcgcc
     6061 gtaccaaacc aacctttcag actgtaggga tctgccggat tacccgcata acaaagcacc
     6121 caatagacca gcagcaggat cagaccggca agaaaagctt tccgggcttt caggtaatac
     6181 accaatacgg aggcaaagaa ataggcgagg gctatccgtt gtaatacacc caatacccgg
     6241 actcctttca cggattcgat gatcgtaccc gctttgtctt tgtagtaatc tatccaggta
     6301 aagcccttga aaatggtttc tttctggaaa tctgtagtgc cttccaccag ccggttgacc
     6361 tccgttacaa agggccacca attgaggaaa agaccgatca gaaagatcag ggccgtgcgt
     6421 ttcaacactt ttttccagaa aagcccgtca ccccccgctt ccagacgggg cattacaaac
     6481 gacatcgcat tgccgacggc aaataaaaag aagggaaaaa ccaggtcggt gggggtcagg
     6541 ccatgccagg gtgcatgttc cagcggagca taaatggatc cccaggaacc ggggttgttt
     6601 accagtatca tcaggcagac cgtggctccg cgaaatacat cgagggaata aaagcgttga
     6661 ttcacagcat tcgttttggt gattgaaggt agtttaaagt tccaattcct gaatccttcc
     6721 aatcggccca ttttcacagc tttttcatca aaaaacaggg ctcttcgaaa tttaaagtta
     6781 tcagactcgt aattctactt atagttatca acttatgggg gtaaatcctt caaaatccga
     6841 tgcttttatt cgttgcccac tcacatcttg gactatattt gcctccaaat tggggaatac
     6901 tggttaaaag tgttccgttt tttaacataa agttatataa atcagtcatt tataataaaa
     6961 atatgtgact gaagtggcga agccatgaat aaaatttatt gagatttgtc aaagaagtgg
     7021 ttggccattt atacccggcc aagatgggaa aaaaaagtga accaactcct gaccgagaaa
     7081 ggattcgaaa gttactgtcc gctcaataaa gtaaaacgga aatggagtga ccggatcaaa
     7141 gtggtggaag aacccctgtt taaatcctac gtttttgtaa aagtgtcgga agacgaccgc
     7201 acggaagttc ggatgaccac cggagcaatt aattatgtgt actggaacgg gaaaccggct
     7261 gtcattaaag aaaaggaaat cagtgccatc cgtcgtttcc tgaatgaaca tgaatatgtg
     7321 gaagcaagac ccatggatgc gaaagtgaac caacgggtcc gaatcatcaa aggaaccttc
     7381 atggatcagg aaggaaaaat cctggaatta agacataaaa ccgctaaaat cgccattgac
     7441 agcctgggct atattttagt cgcctatatt gacagaacta agctaacttc ggcgctgccc
     7501 gaatgacaac ctaaccttat ttttttgcag tccgctgctt tcattaaaag acacctgtaa
     7561 tgaaattcac ccggttttta ttactgcttg cactacccct ttattttatt tcctgtaagc
     7621 ctttacaaaa gatccctaat tacctggaaa atgtgaatga ttccaccgga aagggcgtgg
     7681 taaaagtggt tgaactgaaa attcagaaaa acgacctcct gtctatacag atattcagtc
     7741 tcagcaccca gccagataag gccgatatcc tcttcaacca gcccaccctg ggtgcggccg
     7801 gcggagctcc cggatacctg gtcgataata acgggaatat tgaacaccac cgcctggggg
     7861 ttattcatgc agaaggctta accaaacagc aactggccga tgaagtaaag aaaaggctga
     7921 aagaacctgt ggaagtactg gctgatccaa ccgtactcgt gcggtttatg aatcttaaag
     7981 taacggtact gggtatggtg ggacaggaag gcccgatttc tgtacctgga gaaaaactga
     8041 atatttttga agccgtggga ctggccggtg gtattaatga tttcggcaaa aagaataccg
     8101 tgaaagtaat ccgggaaacc gatggccagc gggaagtggg attcctggac ctgacctcac
     8161 aaaaagtctt tgaatcacct tattataacc tggtgcagaa tgacctcgtc atcgtggaag
     8221 ccaccagcca gaaacaaacc gaacagcagc agaccaagat catgcagaag atcacttttg
     8281 cctttaccat tgtaaccgcg gtggctgccc tttcaaatat ttttatcagg aactaatcct
     8341 cttacgaatt tttattcctt atcatggaag aaacgcacat aaatccgaat tataatccaa
     8401 ataaaagtga tctctccagc ctgagtttca gggatctctt ttataagtat atccggtttc
     8461 tccccgtctt tattttatcc gtggccctca tgcttcttgg cgcttattta tacctgcgct
     8521 ggaccgtacc ggtgtattcc gcatccggaa gtatgatcat taaagatgaa gggaaaaaca
     8581 ggggcgccgg agataaagtg gaagaactgt tcagcagcaa caaggcccag aatatccaga
     8641 gtgaaattga gatactgaaa tcacgtccgc tgatgcagcg cgtggtgatg aaactcaacc
     8701 tgcaattcag ttactacgcc attggtaagg taaaaaccat caatgtctat aaccagggcc
     8761 ctttcctgat cagggccttg aacataacgg actcctccca gccctttatc ttcaagatcc
     8821 gttttgtcaa cgataccgat ttccggatca atgaagaagc cacggtgtat catttcggac
     8881 aggaaataat aaaccgctat ggcagttttg tatttattaa aaatgtcggc cttccggcca
     8941 acaatatata cagtgtgcaa tgggtcccta ctttccaggc ggcagggggg tatgcatcca
     9001 ccctgcagat catgcccaaa accatcggaa ccggtatcct gaatatcagt atgcgttgtc
     9061 cgaacccgca cctgggctcc gccattctga atggattgat ggaggaatac ggggcttaca
     9121 gtcttgaaca gaaaaagctt tcttcagatc agatcctggc ctttatcgag ggcaggctgg
     9181 tggatatcgg caccaagctg gacagcgtgc aacgcatgta cctggattac cagacccgtt
     9241 acaacatgat tgatgcggat gtgcaatcct cttcctattt tggcatcatc aatgaatcgg
     9301 ataaaaccat caatgaacag aactaccgga tatcagtagc cgaaaaaatt gatgagtacc
     9361 tgtccgataa aaaaaatgct tttgctaaaa tcgtggtgcc ctccaccctg gggttggatg
     9421 atcctaccct caacgacctg gtgaacctgt acaataaagt acaactggaa cgtaaacaac
     9481 tggtggacgg caacgtaccg gccgggcacc ccttagtaaa agatgccgac aaccaggtag
     9541 agaaaatcag gttaagcata agggagaata tccgcaatat caacaaggct tcagcggacc
     9601 tggtatcgga tgtgcggaaa cgcagtaacc tgggagaaga acaactcagg gcgatgccgg
     9661 ttaaaataaa agaactggcc gaaatcaaac gccaggtgga aacctaccag gacctgttca
     9721 aactctttac ccagaagaaa gaagagaccg cgatctcccg cgcttccaat acaccaaatt
     9781 cagaaatcat tgaccggtcc tatccacagg taagcccggt gaaacctgat aaacgttcca
     9841 tacaggccat ggccatcctg ctcggcctgg cgatccccgc tttattcata tttgtgagtg
     9901 aagtgctgaa cgataaagta accacccgct tcgatatcga gaaaatcacc caggccccga
     9961 tcctgggaga aatcgggcac tcctattcag aaagcaccct ggtggtcagc aaaaccaccc
    10021 gcagcatggt agcggaacag ttcaggatca tccgctcgaa tcttcagtat gtgctgaata
    10081 aaaaagataa ggcggtgatc atcactacct cctccttcag cggggagggt aaatcctatg
    10141 tatcaacgaa tatgggtgcc gtactggcac tggcaggaaa acggaccgtg atccttgagt
    10201 tcgacatccg caagccaaaa gtattatccg gactgggtat gtccaaagga cccggtatca
    10261 ccaattacct tgtaggtaaa atgaccaacc tggaagagtt gatccgcccg gtaccggaac
    10321 atgaaaatct ttttgttttg ggttgcggac ccgtgccacc caatccgtcc gagatgttgc
    10381 tcgatccccg gctggacgac atgttcaact ggcttaaaaa tcatttcgat attgtaatca
    10441 ttgatacagc accggtgggc atggtgagtg atgccatgac actgagtaag tttgccgact
    10501 gtaccctgta cctggtacgt cagtcgcata cctttaaaaa gcagattgcc ctgatcgatg
    10561 aattttatat cgccaataaa ctgcccaaag tgtcgatcat tatcaatgac gtgaaactta
    10621 aacccggtta tgggtattac ggctacggcc gttatgggta tggatacggg tacggctatg
    10681 gcagttacta tgaagaagaa acagcgccgg ataacttctt cgagcggata ctggccaaac
    10741 tcgacatccg ccgcctgctg ggtttaaaaa agaaaaaaaa gaaaaacaag taagatgatg
    10801 cgtgtactag taaccggtgg tgcaggattt atcggatcaa acctcgtgga agccctgttg
    10861 aaggacgaaa gagtgagctt tgtccgggtg ctggataatc tctccacagg tttcctgaaa
    10921 aatattgaac cctatatgac ggatccccgg ttcgaattcc tggaagggga tatccgggat
    10981 ttcgacacct gtaaaaaagc ggtggaagga attgatgtga tcacgcacca ggccgcactg
    11041 ggttcggttc cccgttccat ttacgatccc ctcacaacca acggggtgaa tattaccggg
    11101 gcgctgaatg tttttaccgc cgcaaaagaa gccggaatca aacgcgtggt gtacgcggcc
    11161 agttcctcca cctacggaga ccatcccgga ttgcccaaag tagaagacat catcggtaat
    11221 ccgctttctc cctatgcagt cacgaaatat gtaaatgaac tgtatgcccg cgtttatgcg
    11281 agcttatacc agatggaatt tgtgggactg cgttacttca atattttcgg tccccgacag
    11341 aatcccaacg gaccctatgc ggccgtgatt ccgctttttg ccgaagccct gatcaaccag
    11401 aaacctccta ctatcaacgg ggatggttca catagccgcg acttcacttt tgtggctaat
    11461 gcggtacagg ccaatatact ttctctgttt tcaaaaaata aagaggcggt taaccaggtc
    11521 tataatattg cctgtggcga gcaaacctcc ttgttggagt tgtttaatgg actgaataaa
    11581 gaagcgaaaa caaccctgca accgatcttc ggcccggaaa ggttgggtga tgtgaagcat
    11641 agcctcgcgg atatctcgaa ggcgaaacaa ctgctggggt acaatcccct ggtgagtgtg
    11701 gaagaggggt tgaaagtgac gtttggttgg tataagaaaa agtttgaggt ttagttgttt
    11761 agtcgtttag cggtagagtt gcttagaacg ttctaaacga ctaaacgaca aacgactaaa
    11821 cttattcaca tatccctctt tcagaaggac ggtctccaaa aaatgtatgc tggtttagta
    11881 tattgatact taaaccttat attatgcata catattcatt tgagaaatta gaagtctggc
    11941 aattagcaag aaaaatggtg gttagtattt acaagttaac tgctttgttc ccaccggaag
    12001 aaaaatttgg gatgatcagc cagatgagaa gggcgtcggt ttcaatttgt tccaatatcg
    12061 cagaaggtag tggaaggaaa acagcaaagg atcaggccgc tttttataat acgtcttaca
    12121 gcagtttatt ggaactgcta aatcaattga taattgcagt ggacctcgga tggcttcctg
    12181 cggagcagct gatacagatt cgaagagaaa ttgagatttt atcaaataaa cttaattcgc
    12241 ttcgaaattc caggctacag tagtctcaac gggtagaagt ttgatcgtgc taagcaactc
    12301 taccgctaaa cgactaaaca tctaaacgac taaacatcta aacaactaaa ccttcttccc
    12361 catgcccagt tcttctacaa caaccactaa aacgatcatc attaccggtg gagccggttt
    12421 tattggctca catgttgtcc gtctttttgt aacccgatac ccggattaca agatcatcaa
    12481 cctcgatgcg ctgacttacg ccggtaacct ggaaaatctc cgggatattg aaaatgcgcc
    12541 caattatttt tttgagaagg ccgacatcct ggataaggat gccattgaac ggatctttgt
    12601 cctgcatcag ccggatgccg tgatccacct ggccgcagaa agccatgtgg accgttccat
    12661 tatttccccg ctggattttg tgtatacgaa tgtgatcggg acggtgaacc tgctgaacag
    12721 tgcccgtcat ttctggaaag ataattttgc cggcaaacgt ttctatcatg tgtctaccga
    12781 tgaagtgtac ggcgccctgg gtgataccgg cttctttacc gaagacacca aatatgatcc
    12841 gcattccccc tacagcgctt ccaaagcagc cagtgatcat tttgtacggg cttatcatga
    12901 tacctacgga ttgcccgtgg tagtgagtaa ctgttcaaat aattacggcc ctaatcattt
    12961 ccccgaaaaa ctgatccccc tctttattaa taatatcatt accaaaaagc cgctgccggt
    13021 ttacggagat ggattgtata cccgcgactg gctctttgta aaagaccatg ccacggcaat
    13081 tgacaatgtg tttcacagtg gtcagaacgg gcagacctac aatattggcg ggtttaacga
    13141 atggcggaat atcgacctcg tgaaactgct ctgtaagcaa atggatgaaa aactggggaa
    13201 tgcacccggt acaagcgaag tgctgattac ctatgtaaaa gaccgtcccg ggcatgaccg
    13261 ccgctacgct attgatgcca ctaaaatcaa taaagaactg ggctggtccc cctccgtcac
    13321 cttcgaagag ggcctttccc agacaattga ctggtacctg cacaataccg aatggctgaa
    13381 aaacgttacc agcggcgcgt atcagcatta ttatgacgaa cagtataaag acagaaactg
    13441 attaatagtt tagtcgttta gttgtttaga cgtttagatc atactttgat gatcttcttc
    13501 taaacgacta aacgactaaa cgactaaacc ctcaaaccat ccctcatgaa aggcatcatc
    13561 ctcgccggcg gctccggtac ccggctttac ccgatcacca aggccatttc caaacaatta
    13621 atgcccatct acgataagcc gatgatttat tatccgcttt ccgtactgat gatggccggc
    13681 atccgtgaag ttttaattat caccacaccg gaagacaatc cttcttttat tc
//
LOCUS       DAOPMW010000048        13517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4640_length_13517_cov_9.033650, whole genome shotgun sequence.
ACCESSION   DAOPMW010000048 DAOPMW010000000
VERSION     DAOPMW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_13517_cov_9.033650"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..656
                     /locus_tag="PLU11_04245"
     CDS             48..656
                     /locus_tag="PLU11_04245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPN58356.1"
                     /translation="MLLNIHPKDPQPRLIKQVVESLQKGGIIIYPTDTVYGLGCDILQ
                     QKAIERICKIKQVDPKKAQLSFICNDLSHLSEYAKPIPNPTYRLLKEFLPGPYTFILP
                     ASKLVPKILQSKKDTIGLRIPDNSIAQAIIKELGRPILSASLPGTMVEDYTDPEVMYQ
                     NFPHEVDYIIDGGIGGMVPSTIIDCTGDEPELVRQGLGEWNG"
     gene            complement(802..1398)
                     /locus_tag="PLU11_04250"
     CDS             complement(802..1398)
                     /locus_tag="PLU11_04250"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPN58357.1"
                     /translation="MLLPCYKETQKEAGLDEAGRGCYAGPVFAAAVILPKDFYHPVLN
                     DSKQLSEKQREELRPVIEKEALAWAVASVGQQEIDQINILKASIKAMHLALDQLKKKP
                     NYLLIDGNRFYPYKKIPHQCFVKGDGRFASIAAASILAKTHRDEYMVKLHEQFPQYGW
                     NKNKGYGTAVHRKAIEAYGLCDHHRKSFNLLPAQNELF"
     gene            1578..2648
                     /locus_tag="PLU11_04255"
     CDS             1578..2648
                     /locus_tag="PLU11_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPN58358.1"
                     /translation="MTTELNSRSSRPIAIWLLVGVGMIIIQVLLGGITRLTGSGLSIT
                     EWNVVTGTLPPMNEQQWMTEFDKYQQTPQYQLLNSGFTIKDFKFIFFWEWFHRVWARL
                     IGVVFAVPFIIFLIQKRFKPSMVKPMLILFLLGALQGAIGWIMVASGLTGDAIYVKPA
                     KLALHFVFALGLLAYTFWFAMELLVPKEKIVYNKKIHKLTIGILAVLLVQLVYGAFMA
                     GHKAATAAPTWPDINGSIIPPNMFGQEGGLLDLIDNRITIHFIHRGLAYILLAMVLWQ
                     ANRLFKSKATADLKMSNWLPAFLVLLQLGLGIFSVLSSTEIIPNKWGFFEWIAQLHQL
                     VAMALLLSQVWLLYIVRRQLAL"
     gene            2721..4982
                     /locus_tag="PLU11_04260"
     CDS             2721..4982
                     /locus_tag="PLU11_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPN58359.1"
                     /translation="MKAWLKGLYHSFPVQLLFLHFRKYQVLLLFWFVLFSTVSGSFMK
                     TFGADSLFLAPEYLGNVNSISYAIVGASIGMFIMSWNITSFILFSKHIRFLAAATNPF
                     LKFCINNSLIPLSFLVFYFFKATHFLQTKELVKGTEILFFFGGFISGFIFILASSLIY
                     FFRADRSILQRMMPVIAEPKNYITHLSAIKEVYHDKPLISVSSYLETPFRLKAARDVS
                     HYTDEFVVSIFKGHHIAAILSVFAAFLFLIGIGFLLDNTFFQLPAAGSITIFFSILIG
                     VAGAVTYFLQSWSIPYLIGLIILLNVFYKLDWIDPRNKAYGLNYWNKTERPQYTRENL
                     AAMTTPANVNADKQNMLAVLEAWKKKQTDTSGKPLLVLINTSGGGHRSATFTMGVLQA
                     LDSISNGEIMKRTFLISGASGGMIGAAYFRELYLRKQKGENIRLQNKDYISDISGDLL
                     NPLFTSFFARDLIAPAQKFSVGPYRYVKDRGYSFEQKLSENTRGFLDKQLKDYVEEEK
                     NARIPLMFFNSVITRDSRKMIISTQPVRFMMRGEVDSSAIPIADPDAVDFVSFFKQQD
                     PYNIRMLTALRMNATFPVVLPNVWLPSDPVIDVMDAGLRDNYGQETSLRFLSCFEEWI
                     KVNTSGVLLIQIRDRQAGGWEAPYLSDDITDHATKPFLLLQHNWFKMMEYSQNDMLDY
                     FTTVNGIGIHKIAFQYQSGSAENKAALNFHLTKREAKDIMHSIEAENNQKNFERLRVL
                     LTMPAKKASKPVE"
     gene            complement(4966..5862)
                     /locus_tag="PLU11_04265"
     CDS             complement(4966..5862)
                     /locus_tag="PLU11_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPN58360.1"
                     /translation="MSRKILYIINPIAGTRTKKDLQQFIEKRTADAGIPFQVFPSVAS
                     GDYSFLLSIIEEERFTDIVVAGGDGTISQVVSSLMKTKLNFGIIPCGSGNGLALAAGI
                     SKKPEKALEVVLNGKPSLVDGFLVNGHFACMLTGIGFDAKVAHEFSKQKTRGLKTYAT
                     LVSKNFFSADPYPFIVEANGLRFSTEAYFISIANSNQFGNNFTIAPKALLSDGYLDIV
                     IAKRSGKPYLLFNLIKQILAGKLIKMESSLKQPVIYFQTEKIKILNPAEAPMHIDGDP
                     AEMAAEVNVQILKSCFLLIQPV"
     gene            complement(6068..6490)
                     /locus_tag="PLU11_04270"
     CDS             complement(6068..6490)
                     /locus_tag="PLU11_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58361.1"
                     /translation="MKKLFLLITAFIISMTIYGQQRWTVSHQKIVILKNVEEDPEGNT
                     IELSKSSLSLNGNLTFTFKINDGTVNRTLMVDDSSRAGIKSWENIKKTTSISNAELKK
                     LFSGRTKLLFYFTEIPKDPAKAAVVRMRPLHLCTLVIK"
     gene            complement(6533..7582)
                     /locus_tag="PLU11_04275"
     CDS             complement(6533..7582)
                     /locus_tag="PLU11_04275"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPN58362.1"
                     /translation="MFKSKIAGIGKYLPANVVTNNDLLKYMETSDEWIQERTGIQERR
                     YAHRTNETTTTMGVEAAKVAIERAGTTPQDIDFIIFATLSPDYYFPGCGVLLQRAMKM
                     KEIGALDIRNQCSGFVYAMSVADQFIKTGMYKNILVVGAEKHSFGLDFSTRGRNVSVI
                     FGDGAGAVVLQPTEKENQGILSTHLHSDGNSAEILAMYNPGTHANHWSEQKLASFDEA
                     EIGQMFFSHAMIDDGQVFPNMDGPAVFKVAVEKFPAVIKEALEANQRTTADINLLIPH
                     QANLRISQFVQKKLQLRDDQVYNNIMRYGNTTAASVPIALCEAWENGKVKDGDLVCLA
                     AFGSGFTWGSVLLNW"
     gene            7736..9103
                     /locus_tag="PLU11_04280"
     CDS             7736..9103
                     /locus_tag="PLU11_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58363.1"
                     /translation="MKQFLYIICLLLVTNQLAAQKQHFDLVSYTAPKGWKKEVSKEAV
                     IFSINETKKKSWAVIKLVRSNPTEGDIDTDFLSAWTFLSATPFGIAESPVTTEIIEGG
                     GWKIKSGSGKFRFKNADAFTTVTTMSGFGVAFSIQFNSNSEQYVPVLTAFIETMEVDE
                     VKITIADNIPPPVKTDDPPAKSSGFTFTNTNFDDGWTSTAMEDWAEVTKGNIRVLIHY
                     PNKEADAYQSVLKDGDLKAWNVLVSPRYSNLRNFEWKSIQSWQSISFMEGELTENSTG
                     KNVHVVLFKMHYSNGNGRYMEFVTNSKGEYEQAFGAYHSTSYGWEVPADMQWRNKFAV
                     SANDLIGKWGTDNYASLTYYYVNTGADAGTSTTATTNEFTFISASNYQSEHNGASGMV
                     GNIKFSSQTYKGTFTTTNWDITMTNRFQGKPEKFSCQFEAVKGGRILVLTDRQGTVFN
                     LGKIK"
     gene            complement(9168..9317)
                     /locus_tag="PLU11_04285"
     CDS             complement(9168..9317)
                     /locus_tag="PLU11_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58364.1"
                     /translation="MNKQHHIKELEMKIEHIRKEPVKGWLGRISKSVKVRKIEKKIRS
                     LRKGD"
     gene            9508..10641
                     /locus_tag="PLU11_04290"
     CDS             9508..10641
                     /locus_tag="PLU11_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPN58365.1"
                     /translation="MNQPVSQRFLALDVLRGMTVFFMIIVNTPGSWDTTWAPLKHAAW
                     HGFTPTDLVFPTFMFVVGNALSFVEKKWNGLSDGKVLWIITKRTLIIFLLGYLMYWFP
                     FLHWNEAGELVANPIANTRIMGVLQRIALGYFFGALMIRYLKAATAVRLSVVLLLLYW
                     LILLLFGDKADPFNMLTNAGNRFDLWLFGESHLYHGEGVAFDPEGVLSTMPSIANVVF
                     GYAAGKFIAEKGKTWEGLTKLLLTGFALISLALLWHLVFPINKKLWTGSFVLLTVGID
                     CVLLAALSYVTDFLGRTKWTYFFEVFGKNPLFIYLLSEIAAILLWFIPIGEKRTPLYA
                     WLYEHIFRHAGAYLGSFLFALAVVMFCWLVGWIMDKKKIYVRV"
     gene            complement(10642..11637)
                     /locus_tag="PLU11_04295"
     CDS             complement(10642..11637)
                     /locus_tag="PLU11_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN58366.1"
                     /translation="MKLLKWLAVILLVFTVVYFLGPRPSTPKYTNELPTIPATGLALE
                     ELVAKNEARFKIKPDNEARILWANDSAKAVTDYAVVYLHGFSASQEEGDPVHYDFAQK
                     FGCNLFLSRLDAHGIDTTEQLVNFTAAGVWQSAKEAYAIGKLLGKKVILMSTSTGGTL
                     ALKLAAEYPEIAGLILLSPNIEINDPNAWMLNNHWGLQIAHLVKGRYNVVADTTATYA
                     QYWNNRYRMEATVQLEELLESTMKASTFNKVTQPTLLLYYFKDEDHQDDVVKVSAMKR
                     MFNQLGTSAALKRQVPVPNAGDHVLGSYVKSKDIATVQTECEKFATEILKLKEAQ"
     gene            11866..>13517
                     /gene="typA"
                     /locus_tag="PLU11_04300"
     CDS             11866..>13517
                     /gene="typA"
                     /locus_tag="PLU11_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPN58367.1"
                     /translation="MDIRNIAIIAHVDHGKTTLVDKILHATKVFRDNQETGELIMDSN
                     DLERERGITIFSKNAAVVYKDVKINVIDTPGHADFGGEVERVLKMADGVILLVDAFEG
                     PMPQTRFVLQKALHLNLHPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLHFPT
                     YYGSGKNGWFNDTLDQIEGITPLLDGIIKHVPPPRINEGPVQMQITSLDYSSFLGRIA
                     VGKVSRGVLKENQPVVLMQADGSIKKTRVKELYVFEGMGKKKVTEVIAGDLCAVVGIE
                     DFNIGDTIADAENPEALPVISVDEPTMNMLFSINNSPFYGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDTDSGDSFQVYGRGILHLGILIETMRREGYELTIGQPQVIIKEVNGVK
                     CEPFENLVVDVPQEYASKVIDLVTRRKGEMHVMETKGEMQHLEFEIPSRGLIGLRSTM
                     LTNTAGEAVMAHRFVEYKPWKGPIPGRNNGVLISKFQERTTGYSIDKLQDRGTFFVDP
                     GEDVYSGQIIAENIKPGDLVVNATEGKKLTNHRASGSDDATR"
BASE COUNT         3454 a         3110 c         3239 g         3714 t
ORIGIN      
        1 attaatctct tagcacaaaa ttgctaactt taaggactac aattgccatg ctgctcaata
       61 ttcatccaaa ggatcctcag ccacgcctga ttaaacaggt ggtggaaagc ctgcagaagg
      121 ggggaattat tatttatccc accgatacag tgtatggatt gggctgtgat attttacagc
      181 aaaaagcgat tgagcggatc tgcaagatca agcaggtcga tcccaagaag gcgcaacttt
      241 cgtttatctg taatgatctt tcgcacctga gcgaatatgc caaaccgatt cccaatccta
      301 cctaccgttt attaaaggag ttcctgcctg gtccgtatac ttttattctt ccggccagca
      361 aactggtgcc caagattctg cagagtaaaa aagacacgat cggattaagg attcccgata
      421 acagcattgc ccaggccatc atcaaggagt tgggccgtcc gatattaagc gcctccctgc
      481 cgggcaccat ggtagaagat tataccgatc ccgaagtgat gtaccagaac tttccgcatg
      541 aagtggatta tatcattgac gggggtatcg gcggcatggt cccttcaacg atcattgact
      601 gtacagggga tgaaccggaa ctggtacggc aagggttagg agaatggaat gggtagaggt
      661 tgggttggaa gttggaagtt ggaggttgga ggttggaagt tggaagttgg aggttggaga
      721 gttggaagtt ggaggttgtt taggacgaat actctatttg tatattgaag cagtctgaaa
      781 gaagtaagag aagtaaggtt ttcagaataa ttcgttttga gctggcaaca ggttgaagct
      841 tttccggtga tgatcgcata agccatacgc ttcaatggct ttacggtgta ccgccgtccc
      901 ataaccttta ttcttattcc agccatactg cggaaactgc tcatggagtt ttaccatgta
      961 ttcatcccgg tgcgtcttgg ccaggatgct ggcggcagca atggaggcaa accggccatc
     1021 gccttttaca aagcactggt gcggtatttt tttataggga taaaagcggt ttccatctat
     1081 caacaggtaa ttcggtttct tttttaactg atccagcgcc agatgcatgg ctttgatgga
     1141 ggcttttaaa atattgatct ggtcgatttc ttgctgtccg acactggcca cggcccaggc
     1201 aagcgcttct ttttcaatca ccggccgcaa ttcctcccgc tgtttctccg ataactgctt
     1261 ggaatcattc aacacgggat gataaaaatc cttcggcagg atcaccgcag ctgcaaacac
     1321 cggtccggca tagcaacccc gaccggcttc gtcgaggccg gcttcctttt gggtttcttt
     1381 gtagcatggg agtagcacga gtgcaattta caaagttgac aggttgacaa gttagaaagt
     1441 tgacaaggga ccttctaagg agttttgtcg taagtaacgg gatctccacc cttatcaacc
     1501 tgcctgtccg gtaggcaggc ttttcaacct gttaaccttt caacctttaa gcccgcaact
     1561 tgttaacttt gcatcccatg acaactgaac taaactcccg cagttcccgc cccatcgcca
     1621 tctggctcct ggtgggggtg ggcatgatca tcatacaggt attattaggc ggaataaccc
     1681 ggctgaccgg ttcgggttta tccattactg aatggaatgt ggtgacgggt accctgccac
     1741 cgatgaatga gcaacagtgg atgactgagt ttgataagta ccagcaaacc ccacagtatc
     1801 aattactgaa ttccggcttt accataaagg atttcaagtt tatctttttc tgggaatggt
     1861 ttcaccgtgt ttgggcccgt ctgatcgggg tggtatttgc ggtacctttt atcattttcc
     1921 tgattcaaaa acggtttaag ccttctatgg taaaacccat gttgatttta ttcctgctgg
     1981 gagccttgca gggagcaatt ggctggatca tggtggccag cggactgacc ggcgatgcga
     2041 tttatgtaaa accggcaaaa ctggccctgc attttgtatt tgccctgggt ctgctcgcgt
     2101 atactttctg gtttgcgatg gaactgctgg tgcctaaaga aaagattgta tacaataaaa
     2161 agattcataa actgactatc ggcatactgg cggtgttatt ggttcagctg gtgtacggtg
     2221 cctttatggc cggacataaa gccgccactg ctgcccccac ctggcccgat atcaatggca
     2281 gtatcattcc gcctaatatg ttcggacagg aaggcggact gctggacctg attgataacc
     2341 ggatcaccat ccattttata caccggggat tggcctatat cctgctggcc atggtgctct
     2401 ggcaggccaa ccggttattt aaatcaaaag caacggcgga cttaaagatg agcaactggc
     2461 tacctgcctt cctggtattg ctccagttgg gtctgggtat tttttcggta ctctccagta
     2521 cggagattat tcctaataag tggggattct ttgagtggat agcccagctt catcaactgg
     2581 tggccatggc cctgctgctt tcacaggtct ggctactcta tatcgttcgc cgtcagctag
     2641 cactttaaca tttaatcagt tacctgagtt tgcaaaggca atgaattaat gttattttag
     2701 agggaactat taaccaactg atgaaagcct ggcttaaagg actctatcat tcatttcccg
     2761 tccagctatt attccttcat ttcaggaagt accaggtgct cctgctgttc tggtttgtcc
     2821 tgttcagcac cgtgagtggt tcgtttatga agacctttgg agccgattcc ctcttcctcg
     2881 ctccagaata ccttggcaat gtgaactcca tcagctatgc gatagtaggt gcctccatcg
     2941 gcatgtttat catgagctgg aatatcacgt cctttattct gttcagcaag catatccgct
     3001 ttcttgcagc agccaccaat ccttttttaa aattctgtat aaataattcc ctgataccgc
     3061 tgagtttcct ggtattctat tttttcaaag ccacccattt ccttcagaca aaagaactcg
     3121 taaaaggaac cgagatcctt ttcttctttg gtggatttat cagcggcttt atttttattc
     3181 tcgcctcctc cctgatttat tttttccgcg ccgaccggag tatcctgcaa cggatgatgc
     3241 ctgtaattgc ggagccgaaa aattatatta cacacctttc cgctattaag gaagtctacc
     3301 atgacaaacc actgatcagc gtcagcagct atctcgaaac ccctttccgt ctcaaagcgg
     3361 cccgggatgt gtcgcattat accgatgagt ttgtggtatc gatcttcaag ggtcatcata
     3421 ttgccgcgat tctttcggta tttgctgcct ttctctttct gatcggtatt ggcttcctgc
     3481 tggataatac cttttttcaa ttgcctgctg ccggcagtat caccatcttt ttctccatcc
     3541 tgatcggggt ggccggtgcc gtgacgtatt ttttgcagag ttggagtata ccttacctga
     3601 tcggactgat tatcttattg aatgtatttt ataaactcga ctggatcgat ccccggaata
     3661 aggcctatgg cctgaattac tggaacaaga cggaacggcc gcaatataca cgcgaaaacc
     3721 tggcggcgat gactacgcct gccaatgtaa atgcagataa acagaatatg ctcgcggtgc
     3781 tcgaagcctg gaagaaaaaa caaacagata catccggcaa acccttactg gtgctgatca
     3841 ataccagcgg gggaggacac cgcagtgcca cttttaccat gggggtgttg caggcgctgg
     3901 atagtatcag caacggggag atcatgaaac gcactttcct gatcagcggg gcttccgggg
     3961 gcatgatcgg ggcggcttac ttcagggaat tgtacctgcg taaacagaaa ggggagaata
     4021 tccggctgca gaacaaggac tatatcagtg atatttccgg tgacctgctg aacccgctct
     4081 ttacttcctt tttcgcccgt gacctgatag cccctgcgca gaaattttcg gttggccctt
     4141 atcgttatgt aaaagacaga gggtattctt ttgaacagaa actgagtgaa aatacccgtg
     4201 gtttcctgga taaacaattg aaggattatg tggaagagga aaagaatgcc cgcattccac
     4261 tgatgttttt caattcagta atcacccgtg acagtcgtaa aatgattatt tccacccaac
     4321 cggttcggtt catgatgcgc ggcgaagtgg acagcagtgc aataccgata gccgatccgg
     4381 atgccgttga ttttgtatcc ttctttaaac aacaggatcc ctataatatc cggatgctga
     4441 ccgcccttcg gatgaacgcc acttttccag tggtgttgcc gaatgtctgg ttgccctctg
     4501 acccggtaat tgatgtgatg gatgcgggtc tccgggataa ttacgggcag gaaacctccc
     4561 tccggttttt atcctgtttt gaggaatgga taaaagtaaa taccagcgga gttctgctca
     4621 tccagatccg ggaccggcag gctggtggct gggaagctcc ctatttatcg gacgatatta
     4681 cggatcatgc caccaaaccc tttttattgc tgcaacacaa ctggtttaag atgatggaat
     4741 attcccagaa cgatatgctg gattatttta ccacggtgaa tggaatcggc attcataaaa
     4801 tagctttcca gtatcagtcc gggagtgcgg aaaataaggc cgcactgaat tttcacctca
     4861 ccaaacggga agcgaaggat atcatgcatt ccattgaagc ggagaataac cagaaaaact
     4921 ttgaacggct cagggtgtta ttgacgatgc ctgcaaaaaa ggcatctaaa ccggttgaat
     4981 aagtaggaaa cagcttttca ggatctgtac attcacttcc gcagccattt ccgccgggtc
     5041 accatcaata tgcatcgggg cttctgccgg attcagtatt ttaatctttt cggtctgaaa
     5101 atatatgacc ggttgtttca gcgaactttc catctttatc agtttgccgg ccaatatctg
     5161 tttaatgaga ttaaacaata agtaaggttt accggatcgt tttgctatta caatatccag
     5221 gtagccatca gagagcaagg ctttaggagc aatagtgaaa ttgttaccaa actggttact
     5281 gttggcgata ctgataaaat aagcttccgt gctaaaccgg agcccgttag cttccactat
     5341 aaaagggtaa gggtctgccg agaagaaatt tttactaacc agggtggcat aggtcttaag
     5401 accccttgtt ttttgttttg aaaactcatg cgccactttg gcgtcaaaac cgatgccggt
     5461 cagcatacag gcaaaatgac catttaccaa aaacccatct accagggatg gctttccatt
     5521 caatacgact tcaagggctt tttccggttt tttggaaatg ccggcggcca gtgccaatcc
     5581 gtttccggat ccgcagggga tgataccgaa gttcagcttc gtcttcatca ggctgctcac
     5641 cacctggctg atggtgccat cgccaccggc tactacaata tcggtaaaac gctcttcctc
     5701 aatgatggat aacaggaaac tatagtcgcc gctggcgaca gagggaaata cctggaaggg
     5761 aatgccggca tcggccgttc ttttttcaat gaattgctgt aaatctttct tggtccgtgt
     5821 accggcaatg ggattaatga tataaaggat cttacgggac attcgtgcaa aagtacattt
     5881 tcttgctcat gcttcggcaa gcctgcctgt ccggtaggca ggctcagcat gacaactgca
     5941 ccggctcagg gagacaggag ttgctcttgt caccctgagc gtgtcgaagg gtgacgaagg
     6001 caaagtacat tttcttgctc atggttcggc aagctcacca tgacaagaac ttcttcttgc
     6061 taccctttta ctttatcacc agcgtacaca aatgcaacgg ccgcattctt accacagcgg
     6121 ctttggcggg atctttagga atctcagtaa agtagaaaag gagtttagtt cgtccgctga
     6181 acaacttttt cagttcagca ttggagatgg aagtggtctt cttaatattt tcccagcttt
     6241 taatgcctgc ccggctgctg tcatccacca tcagggtgcg gttgacagtg ccatcgttta
     6301 ttttaaaagt gaatgtgagg ttaccattca gggataaact ggatttactc agctcaatcg
     6361 tatttccttc cggatcctcc tccacatttt tcaggatgac aattttctga tgcgatacgg
     6421 tccagcgttg ctgtccgtaa atggtcatgc tgataataaa tgcagtgata aggaggaata
     6481 actttttcat acttgtaaag actgttttat acagggggaa gttgctttcc tattaccagt
     6541 ttaacaatac actgccccag gtaaacccac tgccaaaagc ggccaggcaa accaggtcgc
     6601 cgtctttcac tttgccgttt tcccaggctt cgcataaagc aatcggaaca gaagcagcgg
     6661 tggtgtttcc ataccgcatg atattattat atacctggtc atcccgcagc tgcagtttct
     6721 tttgtacaaa ctgactgatc cgcaggttgg cctggtgggg gatgagcagg ttgatatctg
     6781 ccgttgtccg ttgattggct tccagggctt ctttgatcac ggcggggaat ttctctacgg
     6841 ctactttaaa tactgcggga ccgtccatat tcgggaacac ctgtccatca tcgatcatgg
     6901 catgactgaa aaacatttgc ccgatttcgg cctcatcaaa actggccagt ttctgttcac
     6961 tccagtgatt ggcatgggtg cccggattat acatggcgag tatttccgca ctgttaccgt
     7021 cactatgcag gtgggtggag aggataccct ggttttcttt ctcagtaggc tggagtacca
     7081 cggccccggc accatcgcca aagatcaccg aaacattacg gccccgggtg ctgaaatcca
     7141 gtccgaagga atgtttctcg gcaccaacca ccaggatatt cttgtacatg cctgttttga
     7201 taaactggtc ggccacactc attgcatata caaaaccgct gcactggttg cggatatcga
     7261 gggcgccaat ctctttcatt ttcatcgccc gctggagcaa taccccacaa ccgggaaaat
     7321 agtaatcagg actcagggtg gcaaaaatga taaagtcaat atcctgcggg gtggtgccgg
     7381 cccgttctat cgccacttta gccgcttcca cacccatggt ggtggtggtt tcattggtgc
     7441 ggtgggcata acggcgttcc tggattccgg tccgttcctg aatccactca tcactggtct
     7501 ccatgtattt cagcaggtca ttattcgtaa ctacattggc aggcaggtat ttaccaatcc
     7561 cggcgatctt tgatttgaac atagcaatca tttaaaagag gaagatagta aatgtgcggg
     7621 aaatgaaact attgcaggtg aaacagttgc ttttcggatg aatatgcgct gaattcccct
     7681 ttttacagga agaaactatc cgccagagca cctactttta gttcaaattc aacgcatgaa
     7741 acagttttta tacattatat gcctgctcct ggttaccaat cagcttgcag cccagaaaca
     7801 acattttgac ctggtgtctt atacagctcc gaaaggctgg aaaaaagaag tcagtaaaga
     7861 agcggtgatt ttttctatta atgaaaccaa aaagaaatcc tgggctgtta taaaactggt
     7921 cagaagtaat ccaacggaag gggatattga taccgatttt ttgtcggcct ggaccttcct
     7981 gtccgccaca ccatttggta tcgcggagtc tccggtgacc accgagatca tcgaaggcgg
     8041 gggctggaaa attaaaagcg ggagcgggaa attccggttt aaaaatgccg atgcgtttac
     8101 cacggttacc accatgagtg gttttggcgt ggctttcagt attcagttca attcaaacag
     8161 tgagcaatat gtaccggtac tcacagcctt tattgaaaca atggaagtgg atgaagtaaa
     8221 aatcactata gcggataata ttcctccgcc tgtaaagaca gatgacccgc cggcaaaaag
     8281 cagtggtttc acttttacta ataccaattt tgatgatggc tggaccagta cagcaatgga
     8341 ggactgggcg gaagtaacca aaggtaatat ccgtgtgctc attcattatc cgaataagga
     8401 agcggatgct tatcagtcag tcttgaaaga cggcgacctc aaagcatgga atgtcctggt
     8461 gtcgccccgc tattccaatc tccggaattt tgaatggaaa tcgatccaga gctggcaatc
     8521 gatcagtttt atggaagggg agcttacgga gaacagtacc ggcaaaaatg tacacgtagt
     8581 attatttaaa atgcattaca gcaatggcaa tggccgctat atggaatttg tgacaaacag
     8641 taagggggaa tacgaacagg ctttcggtgc gtatcatagt acatcgtatg gatgggaggt
     8701 gccggccgat atgcaatggc gcaacaaatt tgcagtaagt gccaatgacc tgatcgggaa
     8761 atggggaacg gacaattatg cttccctgac ttattattat gttaataccg gtgccgatgc
     8821 agggacttcc accactgcca ccacgaatga attcaccttt atatcagcca gtaactatca
     8881 gagtgaacat aatggagcca gcggtatggt gggtaatata aaattctcca gccagaccta
     8941 taaaggcaca ttcaccacca cgaactggga tattaccatg accaaccgtt ttcagggcaa
     9001 gccggaaaaa ttcagttgtc agtttgaagc agtaaaaggc ggccggatac tggtgctgac
     9061 ggaccgccag ggaaccgtct ttaacctggg aaaaataaaa taagtgtccg taaataataa
     9121 accgcctctg agggcggttt atttaatttt ttaaggcagg caaattttta atctcctttc
     9181 cgcagggagc ggatcttctt ctcaatcttc cggactttca ctgatttgct gattcttccc
     9241 agccaccctt taacgggttc tttccggata tgctcgatct tcatttcaag ctctttaatg
     9301 tggtgctgct tgttcatttt tcaatgtttt gtttcgaaat tgtagaggaa tttgaacaat
     9361 ataaagatac gataagaaaa agcatcaaga acccctccga agcctttggc gttggagggt
     9421 tcttgatggt tcaattaaag attccatttt atttggaatg ttaacttact cccctgtttt
     9481 ttttatgttc gaacaataat actgtttatg aaccagccag ttagccagcg atttttggcc
     9541 cttgatgtat tgcggggcat gaccgtattc tttatgatca ttgtgaatac gcccggcagc
     9601 tgggatacta cctgggcgcc cttgaaacat gcggcctggc atgggttcac ccccaccgac
     9661 ctggtattcc cgacctttat gtttgtggtg ggcaatgccc tgagctttgt tgagaaaaaa
     9721 tggaatggat tgtctgatgg aaaagtattg tggattatca caaagcggac gctcatcatt
     9781 ttcctgctcg gttacctgat gtactggttt ccctttttac attggaatga agccggtgaa
     9841 ctcgtggcca acccgatcgc caatacccgg atcatgggcg tgctgcaacg gattgccctc
     9901 ggttatttct tcggggccct gatgatccgt tatctcaaag ccgcaacggc ggtccggttg
     9961 tcggtggttt tactattgct ctactggctg atccttttat tgtttggcga taaagccgat
    10021 cctttcaata tgctgaccaa tgccggcaac cggtttgacc tctggttatt cggtgaaagt
    10081 catttatatc atggagaagg agtggctttt gatccggaag gcgtgctgag taccatgcct
    10141 tctatcgcca atgtcgtgtt tggttatgca gccggtaagt ttattgcgga gaaggggaag
    10201 acctgggaag gactgaccaa attattactg actggttttg cattgatcag ccttgcttta
    10261 ttatggcacc tggttttccc gatcaataaa aaactatgga ccggttcctt cgttttgctt
    10321 acagtcggta tcgattgtgt gttgttggcc gcattgagtt atgtgaccga ttttctgggt
    10381 cgtaccaaat ggacctattt ctttgaagtg tttggaaaga acccgctctt tatctacctg
    10441 ctctcagaga ttgcggccat cctgctttgg tttattccca ttggtgaaaa acgaacccct
    10501 ttgtatgcct ggttatatga gcacatcttc cggcatgccg gtgcctacct gggttctttt
    10561 ctgtttgcgt tggccgtagt gatgttctgc tggctcgttg gctggatcat ggataagaag
    10621 aagatctatg tgagggtctg actactgcgc ttcctttagt ttcaatattt ccgtggcaaa
    10681 cttttcacat tccgtttgta cagttgctat gtctttcgat tttacataag aacccagcac
    10741 atgatctccg gcattcggga caggcacctg tctttttaaa gcagccgaag tgcctaattg
    10801 attaaacatc cgtttcatcg ccgatacttt taccacatca tcctgatgat cttcatcttt
    10861 aaaataataa agaaggaggg tgggttgggt gactttatta aaagtcgagg ccttcatggt
    10921 ggactccagt aattcttcca gttgtacagt ggcttccatc cggtatcggt tattccagta
    10981 ctgagcatag gtggcggtgg tatcggccac cacattgtac cggcctttta ccaggtgggc
    11041 aatctgcagc ccccaatgat tattcagcat ccaggcattg ggatcattga tttcgatatt
    11101 cggggataat aagatcagac cggcgatctc aggatactcg gcagccagtt taagggccag
    11161 ggttccacct gttgaagtgg acatcaggat cactttttta cccagcagtt tgccgatggc
    11221 ataggcttcc ttggccgatt gccaaacccc tgccgcagta aaattgacca gttgttcggt
    11281 ggtgtcgatc ccatgcgcat ccagccggct caggaacagg ttgcatccaa acttctgggc
    11341 aaaatcataa tgtaccggat ctccttcttc ctggctggcg gaaaaaccat gcaggtacac
    11401 cacggcataa tctgtaacgg cttttgcaga atcattggcc cacaggatac gggcctcatt
    11461 atcgggcttg attttaaatc tggcttcgtt tttggccact aactcttcca acgccaaacc
    11521 ggtagccgga atcgttggca attcattcgt gtatttaggt gtggaaggcc tggggcctaa
    11581 aaagtaaaca acggtaaata ctaacaggat gacagctaac catttaagca gtttcataga
    11641 aactattttc ctaaaactac tgtaaatatt cctgtttgga aagttaagcc tgtattaaat
    11701 gcgggtttca tttggggccc cgggttttta agcgtacctt cgcgcccggt tttgaggagg
    11761 gatttcggag tcaggagtca atagtataga ccggatttat caagtgttac ctctaaacaa
    11821 caaacgtcta aacaactcaa cctgttcaaa gaccaacatt taattatgga tatcagaaat
    11881 atagcaatca tcgcccacgt agaccacggt aaaactaccc tggttgacaa aattttacac
    11941 gccaccaagg ttttccggga taaccaggaa accggtgagc tcatcatgga cagcaatgac
    12001 ctggagcgcg agcgtggtat caccatcttc agtaagaacg cagcagttgt atataaggat
    12061 gttaagatca atgttatcga tacccctggt cacgccgact tcggtggtga ggtagagcgc
    12121 gtactgaaaa tggccgacgg ggtgatcctg ctcgtggatg cctttgaagg tccgatgcct
    12181 cagacccgct ttgtattaca gaaagccctg cacctgaacc tgcacccgat cgtggtaatc
    12241 aataaagtgg ataaaccgaa ctgccgtccg gatgaagtgc atgatgcggt atttgaactc
    12301 ttcttcaacc tggatgccac ggaagaacag ctgcatttcc ctacctacta cggtagtggt
    12361 aagaatggct ggttcaatga caccctggat cagatcgagg gtatcacacc cctcctggac
    12421 gggatcatca aacacgtacc gccgccccgg atcaatgaag gaccggtaca gatgcagatc
    12481 acatccctgg attactcttc cttccttggc cgtatcgccg taggtaaagt aagccgtgga
    12541 gtactgaaag aaaatcaacc cgtggtactg atgcaggccg atggcagcat taagaagacc
    12601 cgcgtaaaag aattatacgt gttcgaaggc atgggcaaaa agaaagtaac cgaagtaatt
    12661 gccggtgacc tttgcgccgt agtaggaatc gaggatttca atatcgggga taccattgct
    12721 gatgcagaaa accctgaagc cttaccggta ataagtgtgg atgagccgac catgaacatg
    12781 ctcttctcca tcaacaactc tcccttctat ggtaaggatg gtaaattcgt tacctcccgt
    12841 cacctgcgtg accgcctgat gaaggaaacc gaaaagaacc tggccctgcg tgtggaagac
    12901 accgacagtg gtgatagctt ccaggtgtat ggtcgtggta tccttcacct gggtatcctg
    12961 atcgagacca tgcgtcgtga aggatatgaa ctgaccatcg gacagccgca ggtaattatc
    13021 aaagaagtaa acggcgtcaa atgtgagcca tttgaaaacc tggtggtgga tgtaccccag
    13081 gaatatgcca gtaaagtaat tgacctggta acccgtcgta aaggcgaaat gcacgtgatg
    13141 gaaaccaagg gcgaaatgca acacctggaa tttgaaattc cttcgagggg gctgattggt
    13201 cttcgttcca ccatgctgac caataccgcg ggtgaagccg ttatggccca ccgttttgtt
    13261 gaatacaaac cctggaaagg cccgattccc ggcagaaata acggggtgct gatctctaaa
    13321 ttccaggaaa ggaccaccgg atattccata gataaattac aggaccgtgg taccttcttt
    13381 gtggatccgg gagaagatgt atactccgga cagatcattg cggaaaacat taagcccggc
    13441 gacctggtgg tgaatgcaac agaaggaaag aaactgacca accaccgtgc cagcggaagt
    13501 gacgatgcca cccgtat
//
LOCUS       DAOPMW010000049        13475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4659_length_13475_cov_9.343294, whole genome shotgun sequence.
ACCESSION   DAOPMW010000049 DAOPMW010000000
VERSION     DAOPMW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13475
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_13475_cov_9.343294"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLU11_04305"
     CDS             <1..351
                     /locus_tag="PLU11_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPN58368.1"
                     /translation="LDFYSQDTIGIQEKVFAMYLNRANRVIGIFPVSTGGITGTVADI
                     RLILSVALKCGATGIIISHNHPSGSLKPSRMDKELTIKLKEAGCIMDIALQDHIIVVP
                     EVGKYYSFADEGEL"
     gene            380..754
                     /locus_tag="PLU11_04310"
     CDS             380..754
                     /locus_tag="PLU11_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58369.1"
                     /translation="MPHFEDELHEFLNDIYIKLVIIEGNEPYYLNFFYDPQVEKAVFQ
                     LGYFSHVENDVKNYDGEPSRLLYNMFLKACLQIDSNFCFTGNQNDIYRVFAVFRSRVN
                     DCIEKLNFPSHFEFSTGMVIIE"
     gene            complement(763..1446)
                     /locus_tag="PLU11_04315"
     CDS             complement(763..1446)
                     /locus_tag="PLU11_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58370.1"
                     /translation="MDLKEKISAIEKFFNYIKEIDIKDLEIYLNPHLKNLENYSNRIN
                     DKKEAARKLLVALKDSPEKSYLESIFEEVTKFPIPDNISWQEKKLIPRSKVENENRPE
                     YLEAAFGILSYIKEVEIERKFKECFKKLHIYYDKDLKQLIDLMNSVKKNDKLTALVKY
                     NFAFDDLLNKLNSHDNYIEKIITQTDLSMDRNNLIDSIVNSVNYTQKGRNEIRNAKLE
                     ELFRPKKDK"
     gene            1568..1792
                     /locus_tag="PLU11_04320"
     CDS             1568..1792
                     /locus_tag="PLU11_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN58371.1"
                     /translation="MEKRYKLEVEKLGARIKFLRRKKGLSQLDLEIQSGINRTEISKI
                     ENGLKNIEFLTIVRLAVALEVEISDFFDIQ"
     gene            2288..2674
                     /locus_tag="PLU11_04325"
     CDS             2288..2674
                     /locus_tag="PLU11_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN58372.1"
                     /translation="MKKDNVPDLAAADTGKVINELEKSMAHIIVEIIEYVPNSVVTKT
                     ILKKSTGNISVMSFDTGEGLTEKTSPFDTFAQIIEGKAEIVIDKVAHKLETGQGIIIP
                     AHTSNLIKPNGRFKMIITVIKSGYEI"
     gene            complement(2796..3161)
                     /locus_tag="PLU11_04330"
     CDS             complement(2796..3161)
                     /locus_tag="PLU11_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN58373.1"
                     /translation="MHLNQAGSASSGELTKAVPHMVVEIIEYVNNAVVSKTILKKSTG
                     NVTAMSFDTGEELSDKISPYDNFVQIIDGVAELTINKEVHHLKLGNGIIIPAHAQHAF
                     AAKEKFKIISTIIKSGYED"
     gene            complement(3307..6168)
                     /locus_tag="PLU11_04335"
     CDS             complement(3307..6168)
                     /locus_tag="PLU11_04335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN58374.1"
                     /translation="MNKKTALLLRPAQLKKTGHLFELLQVDKADKYSVYTTEKINLGV
                     LKKQYPKLPGGFFTAINRFTESNIDLLKAELFHALKKQQAINETEFRKQVQLQLERKL
                     LQELQALKPLLPLLSCYHQSPVPGDDKRLKTAPCIFSTYTPQLSFEVKHSSGGWLSLE
                     ILIVLNGTAYPLSQFSQYGFLLENKNEYFLLKFPDYQVLNRVQSFDWEQEHFTDARFI
                     TEVLEPLTGYPVNRNNLLKTDCLEVMPTGRVVLSELSGQFLMITPQFDYDGHIVEGSW
                     GETTTLTREGKEISIRRHQDAEKAIRWEVEQLHGSFPNQSNGYYYLSFANAAKHHWFL
                     KAFRHLLDHDIQVLGMDMLKHFRYSTEKPVTHYQFISEEKGWILLSFSLKVGKEEISI
                     KELQKILRTGEQALLLKDGSITLLDEDWQRAYSIIIKHARIENRQIRVPKWVFIALEN
                     GTASGEQIATFIPADWKKKWMNWQQADTPLYPLPASIHASLRTYQQKGYEWMRLLDEI
                     EAGVFLADDMGLGKTLQTICYLAWLAENRTQGNILIICPASLVYNWQNEIRKFAPELK
                     SVVYHGSTREETLFEHEGVQIIISTYGTIRSDEEFILTQSLVAVILDESHTIKNPSSQ
                     ITKLIHQLNADKRVVLSGTPVMNNTTDLFSQLHFVLPGIFGSREFFKREYADPIDLQR
                     DDKKVAALKKLTAPFILRRTKEQVARDLPPKTEITLWCQMDPEQQTVYNDIRDSVKGT
                     LSDIIKKQGLQKSKLQVLEGILKLRQLCNSPELLEEYKNSQIPSIKLNTLISEIENNL
                     SDHKVLVFSQFTSMLDLLSLEMKNRGLSHVMLTGATPPKERDRLVQTFNQEEHESRIF
                     LLSLKAGNAGLNLTAADYVFLFDPWWNHAVEQQAIDRTHRIGQQKSVFAYKMICRDTI
                     EEKILQLQDRKKTLSKELIPEEEGFVKALSEEDVQFLFG"
     gene            complement(6265..6495)
                     /locus_tag="PLU11_04340"
     CDS             complement(6265..6495)
                     /locus_tag="PLU11_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58375.1"
                     /translation="MRLSEFIVLTQEEKRLTVVNEGVPLAKKELSEEMIFLFRLQGFF
                     VETHFSKQTKEIQEYLMFRESPELTPAWKLIQ"
     gene            complement(6754..7254)
                     /locus_tag="PLU11_04345"
     CDS             complement(6754..7254)
                     /locus_tag="PLU11_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN58376.1"
                     /translation="MKTYVRIISVSPKDSVLPVSESIKKQLTRLLKQEDFQKKDLRLT
                     LNLKNQKEGLLTVRWLALRQKKDILYVDLSAYISTYIGETEKNLDQLFQKASEGNMIL
                     LFDEADALFGKRAGSSETDNQNREKTTILLIKKMESYKGSILLICKADGCSFLPARLN
                     FTRISG"
     gene            7559..8857
                     /locus_tag="PLU11_04350"
     CDS             7559..8857
                     /locus_tag="PLU11_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPN58377.1"
                     /translation="MNNTLDPRLMHPREQITMVIGRIYRRGLTTTSGGNISVIDDNGD
                     IWVTPSAVDKGSLTVDDIVCVKKDGTIIGRHKPSSEFPFHKSIYEKRPEIKAVIHAHP
                     PALVSFSIVRKIPDTRIVSQARHVCGPIGYAAYRLPGSQALGDIIAEEFQQGFNAVIM
                     ENHGTVVGGTNLADCFLRFETLEFCARTILYAHMIGEPSYLSEEEITAFDQQLPELLP
                     EMDETIYSSSERAVRTEICQIVQRACSQGLMTSSYGTISRRWEGNDFLITPTDVPRWD
                     MQPKDIVQIKDGKREKGKLPSRSTHLHQLIYETHPEINSIILTQTPYLMAFAASDAVF
                     NVRTIPESWIFLQDVYTVPFGGQFNGSQMIPEMLSKNSPALLVKNDCAIVTGDKLLQT
                     FDYLEVAEFSAKSLIMSASLGQLVPINDEQVDELRSAFLK"
     gene            complement(8858..10873)
                     /locus_tag="PLU11_04355"
     CDS             complement(8858..10873)
                     /locus_tag="PLU11_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPN58378.1"
                     /translation="MVKRWFFYGFPARSVTGSNNKDGISTLGKQSNSMRIYFFILCFV
                     ALNSTAQTPRNLLQQKAGPGQLVKILDAGKDWIRYPAYKDRTEWDKLTGNLKSQLIKE
                     GEAYLNYAWKLVTATQYLEYERSGNRTAMEDPAGSNNRALSRLIIAELAEGKGRFTDP
                     IINGVWAACDMSSWVASAHLPVQQSKRSLPDFREEIIDLGSSDMGSFFSWTWYFLHEE
                     FDKINPVISARLKQQIRQRILVPYMERNDFWWQALQEKPGRLVNNWNPWCNFNVLTCF
                     LLIEDDPVRQAAGVYKTMQSTDQFINYVKEDGACEEGPSYWGHAAGKLYDYLELLSRA
                     TGKQIHLFEHPMIRNMGEYIVRSYVGKGWVVNFADASAKGGGDAGLIYRYGKAVNSMD
                     MQSFAAYLVQDKKGITEITTNRDFFRTLENLSIVKEIIATKAALPTAAESWYPQTEFC
                     YIKNNNGLFLAVKGGFNNESHNHNDAGTFSLYANETPFFIDAGVGTYTRQTFGPERYT
                     IWTMQSEYHNLPLINGKPQVFGAQYKATHTRFDAAKKQFSTDLAKAYSNEAKIKTWIR
                     NYTLEGNSLSIEDRFDLEATLAPNALHFLVWAQPVLVNEGIIQLEKEGTRLSLNYDKS
                     IFSYALDTIPQTDKRLSNVWGETIYRIRLTAKKMQSKGNYRVIIRKE"
     gene            complement(11039..11359)
                     /locus_tag="PLU11_04360"
     CDS             complement(11039..11359)
                     /locus_tag="PLU11_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58379.1"
                     /translation="MSNRKLVQSAAFLLLFSFTASAQTSSGNTDMAQSATTTTEEPRA
                     RQLMDRLETIKGIDRSTLNSNERKDLRKEVKEIRKEMKAASGGVYLSVGAILLVILVL
                     ILIL"
     gene            11851..12411
                     /locus_tag="PLU11_04365"
     CDS             11851..12411
                     /locus_tag="PLU11_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN58380.1"
                     /translation="MKLFIKNMVCIRCKMVVKDQLQKLGYHPVIVELGEADITEDLSA
                     AELEQVRTALMHSGLELMEDKKSILIQKIKNIIIELVHYSEEPLVVNLSEFLHQKLDH
                     NYTYLANLFSQVQGTTIEKYFISHKIERVKELLVYNELNLTEIAYLMHYSSVAHLSAQ
                     FKKVTGLTPSHFKQLREQRRKMLEEL"
     gene            12547..12795
                     /locus_tag="PLU11_04370"
     CDS             12547..12795
                     /locus_tag="PLU11_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58381.1"
                     /translation="MSLLYNKKKNVSLELYASALRNENDGLYDAAVSEYEQALLEQER
                     LRFKDARLIAGIHEKLKVLHTIISYNNNFQAALIKPAV"
     gene            12848..13000
                     /locus_tag="PLU11_04375"
     CDS             12848..13000
                     /locus_tag="PLU11_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HPN58382.1"
                     /translation="MSSLLYFVAIILVIGWAVGFFAYSAGSVIHILLVIALVAIIFRL
                     IQGRRV"
BASE COUNT         3691 a         2744 c         2912 g         4128 t
ORIGIN      
        1 ctggattttt actcccagga tactattgga attcaggaga aagtatttgc tatgtattta
       61 aacagggcaa acagagtgat tggcattttt cctgtttcaa caggaggcat tacaggaaca
      121 gttgctgata tccggctaat actttcagtg gctcttaaat gtggggctac tggtataata
      181 atctctcaca accacccatc tggttcctta aaaccttcca gaatggataa ggagctaaca
      241 attaaactta aagaagctgg gtgtattatg gacattgccc tgcaagatca tattattgta
      301 gttccggaag taggtaaata ttacagcttt gcagatgagg gagaattata aatttcgtaa
      361 attaacttca aatattgtta tgccacattt tgaggatgaa ttacatgaat tcttaaatga
      421 tatttatata aagttggtga ttatagaagg aaatgaaccg tattatctta atttctttta
      481 tgatcctcaa gttgaaaaag ctgtatttca acttggatat ttttcacatg ttgaaaacga
      541 tgttaaaaat tatgatggag agccatcaag acttctttat aatatgtttc ttaaagcgtg
      601 cttacaaata gatagtaatt tctgttttac cggcaaccaa aatgatattt atagagtttt
      661 tgctgttttc agatcaagag ttaatgattg tattgaaaag ttaaattttc ccagtcattt
      721 tgagtttagc actgggatgg taattattga ataatcttta aattacttat cttttttggg
      781 acgaaataat tcctctagct tggcatttct aatttcattt cttccttttt gtgtataatt
      841 taccgaatta acaattgagt caattaaatt attacggtcc atactaagat cagtttgagt
      901 aattatcttc tcaatataat tgtcatggga attcaattta tttagtaaat catcaaaagc
      961 gaaattatat tttactaagg cagttagctt gtcatttttt ttcactgaat tcattaaatc
     1021 aattaattgc tttaggtctt tatcatagta aatatgtaat tttttgaaac actctttaaa
     1081 tttacgttca atttctactt cttttatgta gctcaaaata ccaaaagcag cttccaggta
     1141 ttctggtcta ttctcatttt caactttgct tctagggata agttttttct cttgccaaga
     1201 aatattgtct ggtattggaa attttgttac ttcttcaaaa atcgattcca gatatgattt
     1261 ttcaggggaa tcctttaatg ccacaagtaa ctttcttgca gcttcttttt tatcgtttat
     1321 tctgttggaa tagttttcaa gatttttcag atgtgggttt aaataaattt caaggtcttt
     1381 tatatctatt tctttgatat aattaaagaa cttttctatt gcgcttattt tttcttttaa
     1441 atccatttta tttaatctag ggtttaaagt gagttaatta gctctatgag gaagttacgg
     1501 aattttaaaa tatgtgagtt ataactcact tttttagcct gtattttctt cgaccttttc
     1561 cccataaatg gagaagaggt ataaattaga agttgaaaag ttgggagcaa gaattaaatt
     1621 cctaagacgt aaaaaggggc tttctcagct cgatttagag attcagtcag gaataaacag
     1681 aacagagatc agtaaaattg aaaatggact taaaaacatt gaatttctta caattgtaag
     1741 gttggcagtt gcccttgagg ttgaaatttc agactttttt gatattcagt agaaagttac
     1801 caattttatt accaatatag caggttttta ctttcaaatt taactacaaa ctattgatag
     1861 tcagtgtatt tgtttgccgt ccaaggattc ataacccgaa ggtcggcagt tcaagtctgc
     1921 tctccggtac aaataaaagg tctcttcaag aggcctttta ttatacaata atgagaattt
     1981 acaaggagca tcggatacat atcccgactg taaacgtcgg gacggcagtt caaatctgct
     2041 ctccggtaca aataaaaggt ctcatcagga ggcctttttt tgtaagtaag gtagcagcac
     2101 attgagcatc ggattcatat cccgagttta cagtcgggat ggcctttcaa ttctgctctc
     2161 cgttatttag cctgtttatt ctgtcataca ggtcacgggt atacctatct ccagtatgta
     2221 agaatcttgt aatattcatt tcaaaagcga taaaagcggt gaatgctaca ttcctcaatt
     2281 ttgtagtatg aaaaaggata acgtacctga tcttgcagca gcggataccg gaaaagtgat
     2341 taacgagctg gaaaaatcaa tggctcatat tattgtggaa attattgagt atgtcccgaa
     2401 ttcagtagtc actaaaacta tcctgaaaaa atctaccgga aacatcagtg tgatgtcttt
     2461 tgataccggg gagggactga ccgagaaaac atcccctttt gatacgtttg cccagattat
     2521 tgaaggcaag gcggaaatag ttattgataa agtggcccat aaactggaaa ccgggcaagg
     2581 gatcatcatt cctgctcata cttcaaacct gattaaaccc aatgggcggt ttaaaatgat
     2641 catcacggtg attaaaagcg gatacgaaat ttgacctggc tttcagaccg gttactgtaa
     2701 aaatccggaa atacaatttt cagacaactt atctgttcat cagctctggg agccatgaaa
     2761 atggtttttc cgcatactaa aaaaagggga gccggttaat cctcatagcc gcttttaatg
     2821 atggtggaaa tgattttgaa tttctccttg gcggcgaatg catgctgcgc atgggcaggt
     2881 atgataatgc cattccccag tttcagatga tgtacttcct tattgatagt gagttcggca
     2941 acaccgtcaa tgatctggac aaaattatca tatggagaaa tcttgtcact gagttcttcc
     3001 ccggtatcaa atgacatggc cgttacattg ccggtcgatt ttttcagaat ggttttactg
     3061 actacggcat tgtttacata ttcaattatt tcaaccacca tatgcggaac ggctttcgtt
     3121 aactcaccgc tgctcgcgga accggcctgg tttaagtgca tatgtaaagt ataggtggtt
     3181 aaaataatgt cttacaattt agcgataaag aaactactgt ggaaatatat gtttgtgcgt
     3241 aagcgcgcca aatcaagaat attcaacaaa cagggagtat agctttattg acaattgcgg
     3301 tatcacctat ccaaacaaaa attgcacatc ttcttctgat aatgctttta caaaaccctc
     3361 ttcctccggt ataagttcct ttgataaggt ttttttccgg tcctgcagct gcaagatctt
     3421 ttcttctatg gtatcccggc aaatcatttt ataggcaaaa acgcttttct gttgacctat
     3481 gcggtgggta cggtcaatcg cctgttgttc caccgcgtga ttccaccagg ggtcgaacag
     3541 aaacacataa tcggcggcgg tgagattcaa acccgcattt cccgctttta aactcagcag
     3601 gaagatccgg ctctcgtgtt cttcctggtt aaaagtctgt accaggcggt cccgctcttt
     3661 gggaggggtg gcgccagtca gcatcacgtg cgataaacca cggtttttca tttccaggga
     3721 taacaggtcg agcatcgatg tgaactggga aaacaccagt actttatgat ccgacaggtt
     3781 attttcgatt tcgctgatca gtgtattcag tttgatggag gggatctgac tgtttttgta
     3841 ttcctccagt aactccggag aattacaaag ctgcctgagt ttgagaatac cttccagtac
     3901 ctgcaattta cttttttgca agccctgctt ttttattata tcactgagtg tgcctttgac
     3961 cgaatcccgg atatcgttat agaccgtttg ttgttcgggg tccatctggc accagagggt
     4021 tatttcggtt ttgggaggca ggtcacgcgc cacctgttcc ttagtccttc tcagaataaa
     4081 tggagcggtt agttttttca gggccgccac tttcttgtca tcccgctgca gatcgatggg
     4141 gtccgcatat tcgcgtttga aaaattcacg ggagccaaat atgcctggca atacaaaatg
     4201 cagctgactg aacaggtccg tcgtgttgtt catgactggt gtaccgctca gtaccaccct
     4261 tttatccgca ttgagctggt ggatcagttt ggtaatctgg gaagatgggt ttttgatcgt
     4321 atggctttca tccagaatca cggctaccaa tgactgggtg agtatgaatt cttcatcgga
     4381 ccggatcgtt ccataagtgc tgataataat ctgcacgcct tcatgttcga ataaggtctc
     4441 ttcccttgta ctcccatggt acacgaccga ttttaattcc ggggcaaatt tccggatttc
     4501 gttctgccag ttgtaaacca atgatgccgg acaaataatc aggatatttc cctgtgtcct
     4561 gttttcagcc agccaggcca aatagcaaat agtttgcagc gtcttgccca atcccatatc
     4621 atctgccagg aataccccgg cttcaatttc atccagcagc cgcatccatt catacccttt
     4681 ctgctggtac gtacgcaggg aagcgtggat cgacgcgggc agcggataca aaggggtatc
     4741 ggcctgctgc cagttcatcc actttttttt ccagtcggct ggaataaaag tggcgatctg
     4801 ttcaccggag gctgttccat tctctaaggc aataaagacc catttaggaa cccggatctg
     4861 cctgttttcg atccgggcgt gtttgatgat aatgctatag gcgcgctgcc agtcctcatc
     4921 cagtaaggtg atcgatccgt cttttaacag taaggcctgt tcacctgtac ggagtatttt
     4981 ctggagctct ttaatactga tttcttcttt tcccactttc agggagaagg ataaaaggat
     5041 ccaacccttt tcttcactga taaattgata atgagtaaca ggcttttcag tactgtaccg
     5101 gaaatgtttc agcatatcca ttcccaatac ctgtatatca tgatccagca aatggcggaa
     5161 agctttcaaa aaccaatgat gcttagccgc gttggcaaaa ctcaggtaat aataaccatt
     5221 ggattgattg ggaaaactgc catgtaattg ttccacttcc catcggattg ctttttccgc
     5281 atcctggtga cgacggatac tgatttcttt tccttcacgg gtgagggtgg tggtctctcc
     5341 ccaactgcct tccacaatat gaccatcata atcaaactgg ggggtgatca tgagaaactg
     5401 accactgagt tcactcaaca ccacgcgtcc cgttggcatt acttccaggc aatccgtttt
     5461 gagcaggttg ttccggttaa ccggatagcc tgtcaggggt tccagtactt ctgtaatgaa
     5521 ccgggcatcg gtaaaatgtt cctgttccca atcaaaggat tgtacccggt tcagtacctg
     5581 gtaatcgggg aacttcaaca ggaagtattc gtttttgttt tccagcagga aaccatattg
     5641 actgaactgg gacaggggat aggctgttcc attcaataca ataagaattt ccagggaaag
     5701 ccagccgcct gagctgtgct tcacttcaaa actcagctga ggagtatagg tgctgaaaat
     5761 gcaaggggct gttttaagcc gcttgtcatc gccgggtacg ggtgactgat gataacagga
     5821 gagcaggggc aacaggggtt taagtgcctg aagctcctga agtaatttcc gttccagttg
     5881 taattgaacc tgtttgcgga attccgtttc attgatggcc tgttgttttt tcagggcatg
     5941 gaataattca gctttcagta aatcaatatt actttctgta aaccggttga tagccgtgaa
     6001 aaaaccgccg ggtaatttcg ggtattgttt tttcagcaca cccaggttga ttttctccgt
     6061 ggtatatacc gagtatttat cggccttgtc cacctgcagt agttcgaaca gatgtccggt
     6121 tttctttaac tgggcaggac gtaataacag ggcagttttt ttgttcatcg ggtgctggta
     6181 aaaagtctgc aaataaagga aatcccggtc agattcaata gaacgctgac cggtttcttt
     6241 cctctttttt taccagtgaa taacctattg aatcagtttc cacgcggggg ttaattccgg
     6301 tgattcacga aacatcaggt actcctgaat ttcctttgtt tgtttactga aatgggtttc
     6361 gacaaagaaa ccctggaggc ggaagagaaa gatcatttct tccgataatt cctttttggc
     6421 taaagggacg ccctcattta ctacggttaa tcgcttttct tcctgtgtca gcacaataaa
     6481 ttctgatagt ctcataattg cttgttttaa tacagtgaat aaaaaaaggc aaatacaaaa
     6541 acctgctttt gacaaggatt tctccctgac attggcaaaa aatcaatgaa cttacgaggc
     6601 agaggcaata aaactaatcg gataaaacgg ggccagtgac aggcctgagg cagggacaaa
     6661 ttaagacagt cacctgatat gttcaaattt aatttcccgg aggcaataaa agcgggaaaa
     6721 aggtaaaatt gccaggcggg actgaccgct gggttatccg ctgatccggg tgaaattcag
     6781 tcttgcaggc agaaaggagc agccatctgc tttgcaaatc aataagatag aacctttata
     6841 ggattccatt tttttaatga gcaggattgt ggttttctcc cggttttggt tatccgtttc
     6901 actgctgcca gcccgcttcc caaacagagc atccgcctca tcgaagagca ggatcatatt
     6961 tccttcggag gctttttgga aaagctgatc caggtttttt tcggtttcgc cgatatatgt
     7021 actgatataa gccgataagt caacgtagag aatatctttt ttttgccgga gcgccagcca
     7081 gcgaaccgtt aataaacctt ctttttgatt tttcagattc aatgtcagtc gtaaatcctt
     7141 tttctgaaaa tcttcctgtt tcagtaatcg ggtgagttgt ttttttattg attcgctgac
     7201 aggaagcaca ctgtctttcg gggaaacaga aataatgcgc acatatgttt tcaaagaatc
     7261 cttctgggct aagataaagc caggccagga attcaataat attaacagta aaagaaggcg
     7321 catgtttttt ataaatataa ctaattaatt tattcgttca ggctcctgat agttgtaatc
     7381 acttacaggt tggaacggga tggttaacct ccctttattt ttaattttgt catggaatgt
     7441 tagccctggg tgcagtccct gcaatcacgg ggtggatgag caaagccata tttcctgact
     7501 ttaccggatc ctccttctgc aattaccatc gcccattccg agcaccaatc tgatttttat
     7561 gaataataca ctcgaccccc ggctgatgca tccacgtgaa cagattacca tggtgatcgg
     7621 acggatttac aggcgtggcc tgaccaccac ctccggagga aacatttcag tgatcgatga
     7681 taacggcgat atatgggtga cgccctccgc agtggataaa ggctcactga ccgtggatga
     7741 tattgtgtgt gtgaaaaagg acggaacaat tatcggccgg cataaacctt cttcagaatt
     7801 tccttttcac aaatcgattt atgaaaagcg cccggaaata aaagccgtga ttcatgcgca
     7861 cccaccggcc ttggtgtctt tcagtattgt gcgtaaaatt ccggataccc ggattgtgtc
     7921 ccaggcacgc catgtatgcg gacccattgg ctatgcggct tatcgtttgc ccggcagcca
     7981 ggccctcggt gatataattg cagaggaatt tcagcaggga ttcaatgcgg tgattatgga
     8041 gaatcatggc accgtggtgg gcggaacaaa tcttgcggat tgttttctgc gttttgaaac
     8101 acttgagttt tgtgcccgca ccattttata tgcccacatg attggcgaac cttcttatct
     8161 cagcgaagag gagattaccg catttgatca gcaattaccc gagcttttac ctgaaatgga
     8221 tgaaactatt tatagtagtt ccgaaagagc cgtgcggact gagatctgcc agattgttca
     8281 acgggcctgc agccaggggt tgatgaccag ttcctacggc accatctccc gtcgatggga
     8341 gggaaatgat tttctgatca cccctaccga tgtgccccgt tgggatatgc agccaaaaga
     8401 tattgtgcag attaaagacg gtaaaaggga aaaagggaaa ctgcccagcc gctccacgca
     8461 tctgcatcaa ctgatatatg aaacgcatcc ggagatcaac tccattatac tgacgcagac
     8521 tccctacctg atggcttttg cagcatctga tgcagtattc aatgtgcgga cgattcccga
     8581 aagctggata tttctgcagg atgtgtacac cgttccgttt ggcggacagt tcaatggcag
     8641 ccagatgatc ccggaaatgt tatctaaaaa cagcccggcc ctgctggtga aaaacgactg
     8701 tgccattgta accggggaca aactgcttca gacttttgac tacctggaag tggctgagtt
     8761 cagtgctaag tcgctgatca tgtcggccag tctgggtcag ctggtgccaa tcaatgatga
     8821 gcaggtggat gaattaaggt cggccttcct gaaataatta ttccttacgg atgatgaccc
     8881 ggtaattccc ttttgactgc attttttttg ccgtcagccg gatccggtaa atggtttcac
     8941 cccatacatt cgataaccgc ttatctgttt gcgggatggt atccagggca taactgaaaa
     9001 tggatttatc ataattcagg ctgagtcgcg taccttcttt ttctaattgg attattcctt
     9061 catttactaa gacaggttgc gcccatacca ggaaatggag tgcattgggg gcaagggtgg
     9121 cctccaggtc aaagcggtct tcaatggaca gactgttgcc ttccagtgta taattcctga
     9181 tccatgtttt tatcttggct tcatttgaat aggctttagc taaatcggta ctgaattgtt
     9241 ttttagctgc atcaaaccgg gtatgggtgg ctttatattg ggcaccaaag acctgtggtt
     9301 tcccattgat cagcggcaga ttatggtatt cgctttgcat cgtccagatc gtatatctct
     9361 ccggaccgaa agtctgccgg gtatacgttc ctacacccgc atcaatgaag aaaggcgttt
     9421 cattcgcata gagcgaaaaa gtacccgcat cattgtgatt atggctttcg ttattaaatc
     9481 cgcctttcac tgccaggaaa aggccgttat tatttttaat ataacaaaac tcagtttgag
     9541 ggtaccagct ttctgccgcc gttggtaaag ccgcttttgt ggctataatt tcttttacaa
     9601 tggacaggtt ctccagggtt ctgaaaaaat cccggttcgt agtaatttcc gtaatgcctt
     9661 ttttatcctg tacgaggtaa gcggcaaaag attgcatatc catactgtta accgctttcc
     9721 cataacggta tataagaccg gcatcaccgc cccctttggc ggaagcatcc gcaaagttta
     9781 ccacccagcc cttacctaca tagcttcgga caatgtactc acccatattc cggatcatcg
     9841 ggtgttcaaa taaatggatc tgttttccgg tggcgcgact gagtaattcc aggtagtcat
     9901 atagtttgcc ggccgcatga ccccaatagg aagggccttc ctcacaggcg ccatcttctt
     9961 ttacataatt aataaactga tcggtgctct gcatggtttt atatacaccc gctgcttggc
    10021 gaaccgggtc atcttctatc agcagaaagc aggtcagcac gttgaaattg caccagggat
    10081 tccagttgtt aaccagccgt ccgggttttt cctgtaatgc ctgccaccaa aagtcatttc
    10141 tttccatata agggacaaga atccgttgcc ggatctgttg ttttaatcgg gcggaaatca
    10201 ccgggttgat tttatcaaat tcttcgtgga ggaaatacca ggtccaggaa aagaaagaac
    10261 ccatgtctga tgacccgagg tcaatgattt cttcccggaa atcgggcaga cttcttttcg
    10321 actgctgtac tggtaagtgg gcagaagcca cccaggacga catgtcgcag gccgcccaga
    10381 ccccattaat gatcgggtct gtaaaccgac ctttgccttc agccagttcg gctatgatca
    10441 atctgctgag tgcccggtta ttggatccgg cgggatcttc catggcggta cgattgccgc
    10501 ttcgctcata ttcgagatat tgcgtggcag tgaccagctt ccaggcatag ttcagataag
    10561 cttctccttc tttgatcagt tgtgacttta aatttccagt aagtttatcc cattcagtac
    10621 ggtccttgta agcgggatat cggatccagt ctttgccggc atccagtatt ttaactaatt
    10681 gccccgggcc agccttttgt tgaaggaggt ttctgggagt ctgggctgta ctattcaatg
    10741 cgacgaagca tagaataaag aaatagatac gcatggagtt ggattgtttt cctaaggtac
    10801 tgattccgtc tttattattt gaaccggtga ctgatctggc aggaaaccca taaaaaaacc
    10861 accgctttac catgcggtgg atataaggat taatgattaa cgcaacccga tttattgtat
    10921 gatgctgtaa cagggcttaa ataactgaag tcaatcagtt gatttatatg aatcctgccg
    10981 gctgcctgaa caatttacca ggaagatcag caatatatcg gataagcggg ctataggatt
    11041 ataagatcag gattaatact aatattacca ataggatcgc acctactgat agatacacac
    11101 cgccgctggc agctttcatt tccttgcgga tttcttttac ttctttccgc aggtctttcc
    11161 gttcattact gtttaaagta gaccgatcta ttcctttaat ggtttccaaa cgatccatta
    11221 gctgacgagc ccggggttct tcagtagtag tggtggcaga ctgagccatg tcggtattgc
    11281 cggatgaagt ctgggcggag gcggtaaaag aaaataaaag taaaaaagcg gcactctgaa
    11341 caagttttct gtttgacata aagaagtgtt taaattttta atacaactaa aaaccagtga
    11401 cttagttttc atgtttcggt tattttcctg aaataaattt tgttttcaac ctgagcttta
    11461 gggatgcaac tgatcaggtt tgaacgtgtc tgggtacaaa taaccaggag gataaggtgt
    11521 ttaattgcct gatcaaaggt gaaacaattc cattttatca taattatcca gttatgttca
    11581 tttattacaa ctttcataga tttaaaatgc agaatcgtgt gatttacaac gaaaaatgtg
    11641 ctgatcagcc ttttcccatt tcattgggga aaatggggat ccggcaggac tttttggcaa
    11701 ttcaatcagt ggtacaggta gggtgttttg acaaaagaaa tacctgaacc tgctgatttt
    11761 tcagtatatt tagataagtt catacgccgt taatcacctc ccttacttct ttcacatatc
    11821 attggcgtag tgattattaa attcattttc ttgaaactgt ttattaaaaa tatggtctgc
    11881 atccgctgca aaatggtggt caaagatcag ttgcagaaat tagggtatca tccggtaatt
    11941 gttgaattag gggaagcgga cattacagaa gacctgagcg ccgccgaact ggaacaggtt
    12001 cggacggccc tgatgcactc cggactggaa ttgatggagg ataaaaaaag tatactgata
    12061 caaaaaatta aaaacattat tatagaactg gttcattatt cagaagagcc attggtggtg
    12121 aacctgtccg agtttctgca tcagaaactg gatcataact acacctatct cgctaatctt
    12181 ttttcccagg tgcaggggac cactattgaa aaatacttta tctcccataa aattgaacgg
    12241 gtaaaggaac tcctggttta taatgaactg aacctgacgg agatcgctta cctgatgcac
    12301 tacagcagcg tggcccacct gtcggcgcaa tttaaaaaag tgaccggact taccccttcc
    12361 catttcaaac agctcaggga gcagagaagg aaaatgctgg aagagctgta aaacatataa
    12421 aaatgtttcc ggattctgta ataactatga gtgcagttta actgaacttt attgttgttt
    12481 tggttactta tggactttca ggaatatatc atcaaattta gtttggtttc aacagtattt
    12541 tatattatgt ctttacttta taataaaaag aagaacgttt cccttgaatt atatgcttcc
    12601 gcgctgcgta atgaaaatga cgggctgtat gatgcggcag tgtcggagta tgaacaggcc
    12661 ttgctcgaac aggaaaggtt gcgtttcaag gatgccaggc tgatcgcggg tattcacgag
    12721 aaattaaaag ttttacatac aatcatatct tataacaata attttcaggc agccctgatt
    12781 aaaccggcgg tttagttttt tagcagccgt tttatgaagc gcatgagaaa taaataattt
    12841 taaacacatg agtagcttac tttattttgt tgcaatcatt ctggttatcg gttgggcggt
    12901 tggttttttt gcttatagtg ccggttcagt gattcatatt ttattagtga ttgcccttgt
    12961 tgccattatt ttccggctga tccagggccg gcgggtatag tcatacgaac ctgatttgca
    13021 gtagcgtagc tgatgcctga tcagcataaa taaagccccc gtaaaaacgg gggctttcac
    13081 caaaactaac tgttacgaga aattctgctt tttgcttcta tgcaaatatg tttctcaaag
    13141 atcggttagg tgaaagagct aatcattaca ggctggtcca atttatttac aactttcatg
    13201 cagattgtcc tataggggtg aatcaataaa ctattcgcag aattgtataa accccgatcc
    13261 ggttttttgg agcaacttta aacacctgaa agtaattcaa gtatcactta tttgaaatta
    13321 tagcggactt tgtctgtgtc aacccttcag gtataaccat gatcaaagta aagaaagaag
    13381 ggattttgct ggtaaagaca gcaaacggct tcgagaatga aggggtgctg aatcctgccg
    13441 ccatacgcga agggaaaagt attcatcttt tttac
//
LOCUS       DAOPMW010000050        13459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4670_length_13459_cov_9.260743, whole genome shotgun sequence.
ACCESSION   DAOPMW010000050 DAOPMW010000000
VERSION     DAOPMW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13459
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_13459_cov_9.260743"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLU11_04380"
     CDS             <1..273
                     /locus_tag="PLU11_04380"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58383.1"
                     /translation="MMNMGFSKQVFKGKGTLRLNMRDVLYSQKIKGTIKYSNIDAAFQ
                     QRRDSRQVALGFTYRFSKGKMNNNGQKRKTGGASDEQNRVKTGGDN"
     gene            358..849
                     /locus_tag="PLU11_04385"
     CDS             358..849
                     /locus_tag="PLU11_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPN58384.1"
                     /translation="MPSFDIVSKVEAQALDNAVNVTNKEITNRFDFKGSHVVINLDKK
                     EFLLKLETDDDMKMGQLIDVLINRAHKQGIAPEAFDASKAGAQSGKVWKKEVKVRNGL
                     VQEDAKKIVKLIKDAGLKVQASINDDLVRVTGKKIDDLQSVIQLCKTANLGFPLQYVN
                     MRD"
     gene            1238..1486
                     /locus_tag="PLU11_04390"
     CDS             1238..1486
                     /locus_tag="PLU11_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPN58385.1"
                     /translation="MKKGLHPDSYRLVVFKDMSNGHSFLSRSTAASKETVQWEDGKDY
                     PLIKLEISNMSHPFFTGKNMLVDTAGRIDKFNKKYAKK"
     gene            1606..2784
                     /locus_tag="PLU11_04395"
     CDS             1606..2784
                     /locus_tag="PLU11_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HPN58386.1"
                     /translation="MNKIIFTEEFCQPEKLHPFTLTRQLQDIRVGILTIREKWEKMLG
                     LPSFDKKEDDYKDLSRAVDVDAVIGKYDCFMIHGNVLPSPSLVKAIRLLKNGECIATL
                     DGYNIAYRFSRKQMTDKHRIRVSRVVSFKEAIQTIQYPWDIIRLNDQAIREDFKLLTA
                     RRKSQPISRTNKVIKPGQVFIEKGASVEHCIINASTGPVYIGKEASIMEGCLIRGPFA
                     MGEKSCLKMGTKIYGATTLGPGCVGGGEIKNAVLFGYSNKAHEGYLGDAVIGEWCNLG
                     AGTSNSNLKNNASPVKVWTPEGEVVAGPKCGVMMGDYSRTAINTSINTGTVIGVSCNV
                     FGNGLTPKFIPSFSWGSEGLSRYRFEKAVTDIEGWKKLKGTVISADEKMILQYIFEKF
                     "
     gene            2804..3565
                     /gene="tpiA"
                     /locus_tag="PLU11_04400"
     CDS             2804..3565
                     /gene="tpiA"
                     /locus_tag="PLU11_04400"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPN58387.1"
                     /translation="MRKQIAAANWKMNLTYQDGEKLLDDILDAGISLAPHQHSIFAVP
                     FPYLIMTRSEVAEEFNYHAAAQNCHHKTSGAYTGEVSVEMLQSIGVNYCVIGHSERRE
                     YNAETNDILAAKVNLCLQHDITPIFCCGEPLSVREAGTQNEFVANQLKESLFHLPANS
                     LKGIIIAYEPIWAIGTGKTATTAQAQEIHQFLRSVIADQYGATLANEIPILYGGSVKA
                     NNAVELFACPDVDGGLVGGASLVASDFIAIIKALK"
     gene            3641..4033
                     /locus_tag="PLU11_04405"
     CDS             3641..4033
                     /locus_tag="PLU11_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58388.1"
                     /translation="MQRKNQVLLSFLKIKAVVNGREIYPLSNTKPVVIPVGANNPRIV
                     ITDGYHITTPLKLVYNELPVYCFKVACAISDRQLLFGFLMMAGLYLSGFYTGLLVLKV
                     FSFIPLIYLLTFYYLNRKEFIRLIPVTN"
     gene            complement(4030..5004)
                     /locus_tag="PLU11_04410"
     CDS             complement(4030..5004)
                     /locus_tag="PLU11_04410"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPN58389.1"
                     /translation="MAMMKRFRNMRSEDNTGFGNSSNNSGRFFDRRTGGANVHKKGVS
                     LLNRYSWYHTMLGMPRGKFLSLLLLIYVSVNLVFAGIYYLIGVEHLAGIERSHNLKEF
                     SEVFFFSTQTFTTVGYGRISPTGFLTSAISTFEAFIGLLSFAIATGLFYGRFSRPQAF
                     LKFSDTALVAPYKDGSALMFRMTPFKNNLLSEAEVKLTLAMRVEENGKIVNKFFTLPV
                     EIDKLNSFSLSWTIVHPLNEKSPLAGFTEEDFGNTDLELMVFVKAFDEIFSNTVVARW
                     SYITKEFVWGARFNPMFYAGTDRQSTFLDISLLNSYSPATIPAASNTL"
     gene            5070..6242
                     /locus_tag="PLU11_04415"
     CDS             5070..6242
                     /locus_tag="PLU11_04415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN58390.1"
                     /translation="MARKKILWLCSWYPNRADPFDGDFIQRHARAASLYNDIHVIRLV
                     ADASLRPGKTAFQKQESKGLTEQVVYLGKGKSLAGRFLFYQRWNRIFKDVIKAYLADQ
                     GVPFVVHVHVPYPAGRLALWLKQKYGVPFVVTEHWTIYQPGSEIAYSSQPARMRSLIT
                     RVMRETVLFLPVSRDLAEQVNRLVLPVSAKPIPNVVDTNHFCFQVTDHVKRTKFRYIH
                     VSGMNAQKNPEGLLRAFAAAWQEDPELELWMLGNRDQRLAEFASGLDLPAGVILFGGE
                     VSYRDVAKRMRECDALVLFSQVENAPCVISEALCCGLPVIATRTGGIPEMLNDSNGVL
                     IEPGDETAMKTALLDLKKTYNRYNRAVIAAHASSIYNYEAVGKELSVLYNQVVLKG"
     gene            complement(6246..6503)
                     /locus_tag="PLU11_04420"
     CDS             complement(6246..6503)
                     /locus_tag="PLU11_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HPN58391.1"
                     /translation="MDLKEQFEKAIAESKTLSEKPSNDTLLQLYSLYKQSSEGDINID
                     PPANPFDFVAKAKYEAWAALKGKTNAEAMTAYIELVAKLKN"
     gene            complement(6563..7591)
                     /locus_tag="PLU11_04425"
     CDS             complement(6563..7591)
                     /locus_tag="PLU11_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58392.1"
                     /translation="MNKNRLFTVLLLFISITAGAQEAPVDSIRFFTDEGLIHVNLSTD
                     LRQVQTETRQGVFQQASISMLFPDSTLINETISVSARGNFRRNYCRIPPMMMNFRNKG
                     APRLQSLGKLKLVIGCGINPVDEELLLKEYLVYKIYNMLEDRSFRVRLMRVNYTDTRK
                     KLKPFSQFAFLIEDDGDMARRNGCHKKDEVKMMTEATNRDMMTMVALFQYMIGNTDWA
                     VPTNHNIKLIFDKKNKNTPPYVVPYDFDYCGLVDASYAIPHETIGTEKVTERVYRGFP
                     RTMEELQIQLDIFRSKKDSIFKTINSFELLSSKTRKEMTSYLQEFFELIENKRAVSMA
                     FIDEARVN"
     gene            complement(7669..9306)
                     /locus_tag="PLU11_04430"
     CDS             complement(7669..9306)
                     /locus_tag="PLU11_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN58393.1"
                     /translation="MSKIKHMQRLFLSALFSLLIISGQAQSEVKRPKLVVGLVMDQMR
                     WDYLYRYYARYSETGGFKRLLNQGFSCENTLIPYAPTVTACGHSSIYTGTVPAVNGIS
                     GNGWWDKEKMRAVYCTEDKTVSTVGSSSTLGKMSPRNLLVTTICDELRLATNFKSKVI
                     GVAIKDRGGILPAGHSANAAYWYDNTNGNWITSTYYMNELPKWVTDFNSQKIVEKYYE
                     KGWNIMYPLTTYKQSTADDKSWEAKPFGAVFPYDLKKFIGKDYGKISTTPMGNSMTFD
                     FAKAALTAEQLGTDEITDFLAVSFSSPDYIGHTFGPNSVEAEDGFLRLDQELGAFLNY
                     LDLKVGKGQYTVFLSADHGAAHVPEFMEENRLPGGRAYIGKLVESMNKGLKEKYNLSG
                     IIVSDDNYQIHLNHPLLDSAKAVKAEITEWIIQQLLAAPGIDRAFALKDLNTTPLPVR
                     IREMLNNGYHPARNGDIQFILKPGYIDAYSTTGTTHGLWNPYDAHIPLLWYGWGIKKG
                     KTNRETFMTDIAPTLAALLHIQMPSGSTGAPIPEVLK"
     assembly_gap    9397..9496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9507..10727)
                     /locus_tag="PLU11_04435"
     CDS             complement(9507..10727)
                     /locus_tag="PLU11_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN58394.1"
                     /translation="MISATARTYRNAYAGLSRETWLLSFIMLINRSGTMVVPFMTLYL
                     TSKEMGYSVGEAGYVFGLFGAGAFTGAWLGGRLTDRIGFYPVQLFTLFTGGLLFLVLG
                     QMKTYPLICLFTFLLSFVNEAFRPANSTAIAFYSKTENRTRSYALNRLAINLGWAVGS
                     GLGGILASINYELLFWVDGFTNITAALIMWLLLKPADYKPVAQSPAEKADTLPASKDK
                     LFMLFIGATVLFASCFFQLFTNLPVYFERELHLSKPTIGLLMAFNGILIAVIEMVLIY
                     RLEGKRPVMFYITRGILLVGLSFLLLNLSGIGFMLALIMIITVTFGEILSMPFMNSYW
                     ITRTQVSNRGQYAALYTMAWSAAQTLGPMGGAQVVQYSNFQVLWYITGGLCFAAAGIF
                     HLLNKKERKKLSGL"
     gene            10845..11192
                     /locus_tag="PLU11_04440"
     CDS             10845..11192
                     /locus_tag="PLU11_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58395.1"
                     /translation="MKKLILLTAMAAMALVLSNCSGSKKLAATPKLNFESNLKAVVMS
                     ECAPCHIPAKGGNKKPYDNYANVKTDIDEIIRRIEMNPGERGFMPFRKTTKLSDSTIA
                     LFKQWRADGVLEN"
     gene            complement(11196..11648)
                     /locus_tag="PLU11_04445"
     CDS             complement(11196..11648)
                     /locus_tag="PLU11_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58396.1"
                     /translation="MKLILLFFFSLLACCSPAAAQEFINMPYNKAETALKKAYPGKSG
                     QPAVYTRKDSILVQVSDLTQLIYVFDKKGLCMTETTSTRCDSCYNGYLEVLLNKKIYN
                     WKKINENQYISRYEDNMMIELVPEAEIRTFSIFRADFTREVYELLLKD"
     gene            11747..12217
                     /locus_tag="PLU11_04450"
     CDS             11747..12217
                     /locus_tag="PLU11_04450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HPN58397.1"
                     /translation="MALLEDKIRVKKSGLPGAGKGLFAKSFIPKGTRIVEYKGDITTW
                     KEVDHEDGDNGYIYYVKRNHVIDALRTPEALARYANDARGIVRVKGISNNSEYMEEGL
                     KVYITSVKDIPAGGEILVDYGKEYWDVIRHNIRLEAKKQKELAAKKTKSKKKRA"
     gene            complement(12219..12569)
                     /locus_tag="PLU11_04455"
     CDS             complement(12219..12569)
                     /locus_tag="PLU11_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58398.1"
                     /translation="MKEFLKTLLATFDQSVIDYKAYLAAGKTYRYAQVLKANNRKALQ
                     LLDEHKANLPGFLLQDAEALIAHYVVWTQKWEELAERLQPGPDETFVFPNTITFPRES
                     AARLQAYYGELTGS"
     gene            complement(12607..13125)
                     /locus_tag="PLU11_04460"
     CDS             complement(12607..13125)
                     /locus_tag="PLU11_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPN58399.1"
                     /translation="MQIKKIQSIIYEIRGQKVMLDFDLARLYGTETRSLKQAVKRNRL
                     RFPKDFMFILTKKEWQEVITNCDNLDDSIKYSPSLPFAFTEHGVTMLASVLRSGKAVQ
                     MNIAIVRAFISLKEFALNYQELAHEIKALKKITGNHNGRLNQIYVVLENLLDEKVKQK
                     KWEDRDRIGFRK"
     gene            complement(13274..>13459)
                     /locus_tag="PLU11_04465"
     CDS             complement(13274..>13459)
                     /locus_tag="PLU11_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627862.1"
                     /note="ATP-dependent carboxylate-amine ligase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HPN58400.1"
                     /translation="YELLDFLDPVLDHLGSRHRIANVHRILEHGTGADRQLAVFEQTK
                     NLTDVAKFIQGQYLAGI"
BASE COUNT         3718 a         2969 c         3055 g         3617 t
ORIGIN      
        1 atgatgaata tgggattcag caaacaggta ttcaaaggca aaggaacttt acggctgaat
       61 atgcgtgatg tgctgtactc acagaaaatt aaagggacca ttaaatacag taatatcgat
      121 gccgcttttc agcaacgccg ggattcccgc caggtagcgc ttggattcac ctaccgtttc
      181 agtaagggta aaatgaataa caacgggcag aagcggaaaa caggcggcgc ctccgatgag
      241 cagaaccgcg taaaaacagg gggcgataat taaaacctta tccattaaat cttttccaga
      301 gtccggccgc tgcggccgga ctttttttat atttgtaaaa taccaaaacg aacaactatg
      361 ccatcctttg atattgtcag taaagtagaa gcccaggcac tcgacaatgc cgtcaatgta
      421 accaacaaag aaatcacaaa ccggtttgat tttaaagggt cccatgtggt gattaacctg
      481 gataaaaagg agtttctgct caagctggaa accgatgacg atatgaaaat gggacaactg
      541 attgatgtat tgatcaaccg ggcccataaa caggggattg cacccgaggc ttttgatgca
      601 tccaaagcag gagcccaaag cgggaaagtc tggaaaaaag aagtgaaagt cagaaatggc
      661 ctggtacagg aagacgccaa aaagatcgtc aagctgataa aagacgccgg gctgaaggta
      721 caggcatcga tcaatgatga tctggtacgg gtaacgggaa agaaaataga tgacctgcag
      781 tcggtgatcc agctctgtaa gaccgccaat ctcgggttcc cgcttcaata tgtgaatatg
      841 cgtgactaaa agagggataa acggctattt caggtgcata attaagtgca gaatgggtgc
      901 atatcataat ggctgaaaat ttggaaacag ggttttaaaa agattacatt tggtatacaa
      961 ctgcacgatg tcttagcaat aagacagaaa tgcaagaggg gattcagaag cctttcaaac
     1021 ggtggaaaat ttccggaaac agcccttcaa taaggcggtt ttcagaaagc gtcgggtaag
     1081 taagcggaaa gcgaaagccg gaaactgagc tcacttggcg ctttcatctt tttgcccctt
     1141 tatttggccg aaagccgctt ccggcctata tttgcagccc aaatttatct attcatctat
     1201 acggcaaaat ccgtatagcc ttaaaaatca tagtattatg aaaaaaggac tccatcccga
     1261 cagctaccgg ttagtagttt ttaaagacat gagtaacgga cattctttcc tgagccgttc
     1321 taccgccgct tccaaagaaa ccgttcagtg ggaagatggt aaagattatc ccctgatcaa
     1381 actggagatt tccaacatgt cgcacccctt ctttaccggt aaaaacatgc tggtggatac
     1441 ggcaggacgt atcgacaaat tcaacaagaa gtacgcgaaa aaataagaaa aacattaact
     1501 tgtgcagagc tctttgcgaa agcgaggagt tttgtttttc atggctatac tttaaacctc
     1561 ccggttctcc gggaggttgt ttatttttac acctaattcc attccatgaa caagatcatt
     1621 tttacggagg aattctgtca gccggaaaaa ctccacccat ttacgctgac ccgccagctc
     1681 caggatatcc gggtaggcat cctgaccatt cgtgagaaat gggagaaaat gctggggctc
     1741 ccttcatttg ataaaaaaga agacgattat aaggatcttt cccgtgctgt tgatgtggac
     1801 gccgtgattg gcaaatacga ttgctttatg attcatggca atgtgttgcc ttccccttcg
     1861 ctggtcaaag cgatccggct attgaaaaac ggggaatgca ttgccactct ggacggttat
     1921 aatatcgctt accgtttcag ccgtaagcaa atgacagata aacaccggat cagggtatcc
     1981 cgcgtggtga gttttaaaga agccattcag accattcaat acccctggga tattatccgt
     2041 ttaaatgacc aggccatccg ggaggatttc aaactactga cagcccgtcg gaaatcacag
     2101 ccaatctcaa ggaccaataa agtcattaaa cccggccagg tttttattga aaaaggggcc
     2161 agtgtggaac attgtatcat caatgcatcc acaggacctg tttatatcgg caaggaggct
     2221 tcaatcatgg aaggatgtct catcaggggg ccatttgcga tgggcgagaa atcctgcctt
     2281 aaaatgggta caaagatata tggtgccacc acccttggtc cgggttgtgt ggggggggga
     2341 gagatcaaga atgctgtact ttttggttat agcaataaag cacatgaagg ctacctcggt
     2401 gatgccgtga tcggcgaatg gtgcaacctg ggagcaggta ccagcaattc caacctgaaa
     2461 aataatgcca gtcccgtgaa agtatggaca ccggaaggag aagtggtggc cggccccaaa
     2521 tgcggggtga tgatgggtga ttattcccgg acagccatta atacatcaat caatacaggt
     2581 acggttatag gtgttagttg taatgtattt ggaaacgggc tcacccctaa atttattccg
     2641 agcttttcct ggggcagtga ggggttaagc cggtatcgtt ttgaaaaggc agtgaccgat
     2701 attgaaggat ggaaaaaact aaaaggaacc gtaatttcgg ctgacgaaaa aatgatcctt
     2761 caatatatct ttgaaaaatt ttaaaactgt ataaaaagct acaatgagaa aacaaatcgc
     2821 agccgcaaac tggaaaatga acctgactta tcaggatgga gaaaaactgc tggatgatat
     2881 cctggatgca ggaatcagcc tggccccgca tcagcacagc atttttgcag tgccctttcc
     2941 atacctgatc atgacgcgga gtgaagtggc ggaagagttt aattaccacg cagctgcaca
     3001 aaactgtcac cataaaacgt ccggtgccta caccggtgag gtatctgttg aaatgttaca
     3061 gtctattggc gttaattatt gcgtgatcgg tcacagtgag cgaagggaat acaatgcgga
     3121 aaccaatgat atactcgctg caaaggtgaa tctgtgcctg caacatgata tcactccgat
     3181 cttttgttgt ggtgaaccct tgtctgtacg ggaagctggc acacaaaacg aatttgtggc
     3241 caaccagctt aaagaatccc tttttcattt acctgcaaac agcctgaaag gaattatcat
     3301 cgcttacgaa ccaatctggg cgattggtac cggaaaaacg gcgaccaccg cacaggcaca
     3361 ggaaatacac cagtttctgc gatcagtcat tgcggatcaa tatggtgcaa cactggccaa
     3421 cgaaataccg attctgtatg gcggaagtgt caaagccaat aatgccgttg aattatttgc
     3481 ctgcccggat gtggatggag gtttagtagg aggggcatcc ctggtggcca gtgattttat
     3541 cgcaattatc aaagccctga agtaagcata tagggcagtg ataatgaatc cgtttttatc
     3601 aacaaaacag taagttatta ctgcccctta tcatattgtc ttgcaacgaa agaatcaggt
     3661 gctgctcagt tttctgaaaa taaaagcagt cgtgaacggg cgtgaaatat acccgctaag
     3721 taacacaaaa ccggtggtga ttcccgtggg ggccaacaac ccccggatag tgattacgga
     3781 tggttaccat attactactc ctttaaaact ggtttataac gaacttccgg tttattgctt
     3841 caaagtagcc tgtgccatca gtgaccggca actgctgttt ggattcctga tgatggcggg
     3901 tttgtacctc tccggttttt ataccgggct cctggtttta aaagtcttca gttttattcc
     3961 gctgatttat ttacttacat tctattacct caaccgtaaa gaatttatcc ggctcatacc
     4021 ggtaaccaac taaagggtat tggatgcagc aggaatggtc gccggggaat aactgttcag
     4081 cagtgaaata tccagaaacg tactttgacg atcagttccg gcataaaaca tcggattgaa
     4141 ccgggcgccc caaacaaact ctttcgtaat gtatgaccaa cgggcaacca cggtattgga
     4201 gaagatttca tcaaaagctt ttacgaatac catcagttcc agatccgtat tcccgaaatc
     4261 ctcttctgta aaaccagcca ggggactttt ttcattgagc ggatgtacaa tggtccagct
     4321 aagtgaaaat gaattcagtt tatcaatttc aaccggcagc gtaaaaaatt tattgacgat
     4381 tttcccattt tcctccacgc gcatggctaa tgtcagttta acctcggctt cagagagcag
     4441 gttgtttttg aaaggcgtca tccggaacat cagtgcactt ccgtctttat aaggggcaac
     4501 cagggctgtg tcactgaatt taaggaaggc ctgtggcctt gaaaaacggc cataaaaaag
     4561 accggtggct atcgcaaaac tcagcaggcc tataaacgct tcaaaagtcg aaatggcact
     4621 ggtcaggaat ccggtcggac tgatccggcc atatcctacc gtggtgaatg tctgtgtact
     4681 gaagaaaaat acttctgaga actcctttag attatggctg cgttcaatgc cggccaggtg
     4741 ttccacgcca atcaggtaat aaatgccggc aaaaaccaga ttgaccgata cataaatgag
     4801 taataagagg gaaagaaatt tcccccttgg cattcccagc atcgtgtgat accagctata
     4861 ccggttgagc aggcttactc cttttttgtg cacattggct ccaccggtgc ggcgatcaaa
     4921 aaatcgtccg ctgttattac tgctattccc gaaaccggta ttgtcttcag accgcatgtt
     4981 gcggaacctt ttcatcattg ccatagacaa atgtataatt aatcggcaat ttcttttgtc
     5041 cgcccgtgat actggtttat tttagtccta tggcccggaa aaaaatactt tggctttgca
     5101 gctggtaccc caaccgggcc gatccattcg acggggattt tattcagcga catgcccggg
     5161 ctgcttcctt atacaatgat attcatgtta tacgattggt ggccgatgca tcacttcgcc
     5221 cgggaaagac agcgtttcaa aagcaggaaa gcaaagggtt gaccgagcag gtggtttacc
     5281 tgggaaaagg aaaaagtctg gccgggagat tcctgtttta ccagcgttgg aataggattt
     5341 ttaaagatgt gattaaagca tatctcgcag atcagggagt cccctttgta gttcatgttc
     5401 atgttcctta cccggccggc cggctggcat tatggttaaa gcaaaaatac ggagtccctt
     5461 ttgtggtcac agaacactgg accatttatc agcctggaag cgaaattgct tattccagcc
     5521 aaccggcgcg gatgcgtagc ctgataacac gtgtaatgcg ggagacggtt ttgtttcttc
     5581 cggtgagcag ggatcttgca gaacaggtaa accgcctggt attaccagtg tctgcaaaac
     5641 caatcccgaa tgtggttgat acaaaccact tttgtttcca ggtcacagat catgtgaagc
     5701 gtacaaagtt ccggtatatc catgtttcgg gtatgaatgc ccagaaaaat ccggaaggat
     5761 tgctccgggc ttttgctgca gcctggcagg aggatcctga gctggaatta tggatgttgg
     5821 gtaaccggga ccaacgcctg gctgaattcg cctccgggct ggacctgccg gctggtgtta
     5881 ttttgtttgg tggtgaggtt agttacaggg atgtagcaaa gcggatgcgg gagtgtgatg
     5941 cccttgtatt attcagtcag gtggaaaatg caccttgcgt aatcagtgaa gccctgtgtt
     6001 gcgggttacc ggttattgct acccggactg gggggattcc ggaaatgctg aatgattcga
     6061 atggggtact gatagaaccc ggagatgaaa ccgctatgaa aacagccctg ctggacctga
     6121 aaaaaacata taacaggtac aacagggccg tcatcgctgc tcacgcgtca tccatatata
     6181 attatgaagc agtgggaaaa gaactttcag ttctgtacaa tcaggttgta ttaaagggtt
     6241 agccgtcagt tcttaagctt tgccaccagc tcaatataag cggtcatggc ttctgcattg
     6301 gtttttcctt ttaaagcagc ccaggcttca tactttgctt tggctacaaa atcaaacggg
     6361 ttggccggcg ggtcaatatt gatatctcct tcgctggact gtttgtataa tgaatacagt
     6421 tgcagcaggg tgtcgttact gggtttctca gataaggttt tgctttctgc aatcgctttt
     6481 tcaaattgtt ctttcaggtc cattggtatt tgttttcttg gtataataat gctgacttgt
     6541 ttttagtcca tttctatccg ggttaattaa ccctggcttc atcaataaag gccatgctga
     6601 cggctctttt attttcaatc agttcaaaaa actcctgcag gtaactggtc atttcctttc
     6661 tggttttgct actcagcaat tcaaaactgt ttatggtttt aaatatactg tcctttttgc
     6721 tccggaatat atccagttga atctggagtt cttccatcgt ccggggaaag ccccggtaaa
     6781 cccgttcggt tactttttct gtcccgatgg tttcatgggg tatcgcgtag ctggcatcca
     6841 ccaggccgca ataatcgaag tcatagggaa ccacataggg tggggtgttc ttgttttttt
     6901 tatcaaaaat cagtttgatg ttatggttag tgggtacggc ccagtccgta ttcccgatca
     6961 tatactgaaa caaggccacc atcgtcatca tatcccggtt tgtggcctcc gtcatcattt
     7021 ttacctcatc ttttttatgg catccgttac gcctggccat gtcaccatca tcttctatca
     7081 gaaaggcaaa ctgactgaaa ggtttcagtt ttttacgggt atccgtatag ttaacccgca
     7141 tcagccggac gcggaaactc cggtcctcca gcatattata aattttataa acaaggtatt
     7201 ccttcagtag taattcttca tctaccggat ttattccaca accaattaca agtttcagtt
     7261 tgcccagtga ttgcaaccgg ggggctcctt tattccggaa attcatcatc atcggtggaa
     7321 tccggcaata gttccgtctg aaatttccac gggcactcac actgattgtt tcattgatca
     7381 gtgtactgtc tggaaataac atgctgatgg aagcttgttg aaatacacct tgcctggttt
     7441 cagtctgaac ctgcctcaga tctgtactga gatttacgtg gatcagccct tcatctgtaa
     7501 aaaaccggat gctgtcaacc ggggcctcct gggctcctgc tgttattgaa atgaacaata
     7561 aaaggactgt gaacaggcgg tttttattca tacaaaacaa ttaatgttta gcgatcaagt
     7621 cttgtctgaa aatacaaaaa ctattcctga tccgcacgct tttctgtgtt atttaagcac
     7681 ttccggtatt ggggcaccag tggatccgct gggcatctga atatgtaata aggccgccag
     7741 tgtcggagcg atatctgtca taaatgtttc ccggttggtt ttcccttttt tgatccccca
     7801 gccataccat aaaagaggga tatgtgcatc ataaggattc cataaaccat gggtcgtgcc
     7861 ggttgtacta taggcatcga tatagccagg ttttaaaata aactggatat ccccgttacg
     7921 ggccggatga tatccgttat tcagcatttc ccgtatcctg actggcaggg gcgtggtatt
     7981 caggtctttt aaagcaaatg cccggtctat tccaggggct gccagcagct gttgaatgat
     8041 ccattccgta atttcggctt ttactgcttt ggcagaatca agcaaaggat ggttcaaatg
     8101 aatctggtag ttgtcgtcac tcacaataat gccggataga ttatattttt ctttcagccc
     8161 tttattcatc gactccacca gcttgccaat ataagcacgt cctccgggta atcggttttc
     8221 ttccataaat tcaggcacat gcgcggcacc atgatccgca gaaagaaaga ccgtatactg
     8281 acctttccca acctttaaat ccaggtaatt caaaaatgcg cccagttcct ggtccagtcg
     8341 aagaaatcca tcttctgctt ccaccgaatt ggggccaaaa gtatgaccga tataatccgg
     8401 tgacgagaaa ctcactgcca ggaaatcggt gatctcatct gtgcctaatt gttctgccgt
     8461 caaggctgct tttgcaaagt caaaagtcat gctgttgccc atcggggtgg ttgatatttt
     8521 accgtagtct ttgccaatga atttcttgag gtcatacggg aatacggccc caaatggttt
     8581 tgcttcccag cttttgtcat ccgctgtact ttgtttgtag gtcgttaagg ggtacataat
     8641 attccaaccc ttttcataat atttttcaac gattttctga ctgttgaaat cggtgaccca
     8701 tttgggtaat tcattcatat aatacgtcga agtgatccag ttgccgtttg tgttatcgta
     8761 ccagtaagct gcattggcac tatgtccggc cggcaaaatt cctcctctgt cttttatggc
     8821 cacacctatc accttgcttt tgaaatttgt agccagcctg agttcatcac agatcgtagt
     8881 taccagcaag ttccgcggac tcatctttcc caacgtggaa ctgcttccta ctgttgatac
     8941 cgttttgtct tccgtacaat agacagcacg cattttttcc ttatcccacc agccatttcc
     9001 ggaaatgcca tttaccgcag gtacagtccc cgtataaata gaactatgac cacaggcggt
     9061 aacggttggt gcgtaaggaa tcagcgtatt ctcgcatgaa aatccctggt tcagcaaccg
     9121 tttaaagccg cccgtctcgc tataacgcgc ataataacga tagagataat cccagcgcat
     9181 ctggtccatt accagcccaa ccacgagttt cggccgtttt acctcggact gagcctggcc
     9241 ggaaataatc aacagggaaa atagtgcgga taaaaacagg cgttgcatgt gtttgatttt
     9301 agacaaaatt aagggtttgc ttcttgttgc tgtatgactg ttctgtaaac tatactgatg
     9361 gatagtgaag aattagtttt ttataaccac tgttttnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnactg ttttagttaa agcccggaca gtttttttcg ttctttttta
     9541 ttcagcagat gaaaaattcc ggctgccgca aaacagagtc caccggtaat ataccagagt
     9601 acctggaaat tactgtactg aaccacttgt gcgcctccca tcggtccgag ggtctgggcg
     9661 gcagaccagg ccatagtata taaagccgca tattgccccc ggttcgatac ctgggtccgg
     9721 gtgatccagt aactgttcat aaatggcatg gaaaggattt caccgaaagt gaccgtaata
     9781 atcatgatca gcgccaacat gaaaccgatt ccagatagat tcagcagcag gaatgacagc
     9841 ccgacaagca gtataccccg ggtgatatag aacatgacag gtcttttccc ctccagccgg
     9901 taaattaaga ccatttcaat cacggcgatc aggataccat taaaggccat caataaaccg
     9961 attgttggtt ttgacaaatg taattcccgt tcaaaataga ccgggaggtt ggtgaacaac
    10021 tgaaaaaaac aactggcaaa taaaacagtg gcgccgataa agagcataaa tagtttgtct
    10081 ttagaagcgg gcagcgtatc tgctttctca gcaggacttt gtgcaacagg tttgtaatcc
    10141 gcaggtttta gcagcagcca catgatcagt gccgccgtaa tattagtgaa accatctacc
    10201 cagaataata attcgtaatt gatactggcc agaataccgc ccagtccgct cccaacagcc
    10261 catcccagat taatcgccag ccggttcagg gcataggagc gggtacggtt ttccgtcttg
    10321 ctgtaaaagg cgatggctgt tgaattggct ggccggaaag cttcattcac aaaacttaac
    10381 aagaaggtaa acaggcagat cagcggatag gtcttcattt gtcccaggac caggaataga
    10441 agtcctcctg taaaaagggt gaacagttgc accggataaa aaccaatccg atcggtaagg
    10501 cggcctccca gccaggcacc tgtgaaagca ccggcaccga ataacccaaa aacatatccg
    10561 gcttcaccta ccgagtagcc catttcctta ctggtcaggt aaagggtcat aaacggaacc
    10621 accatggtgc cactccggtt gatcagcata atgaaggaga gcaaccaggt ttccctggaa
    10681 agccctgcat aggcatttcg gtatgtccgg gctgttgcag aaatcataac gtggtaaagt
    10741 taccggattg tccggcatct gggcaaccac ccgtataaac caaccgattg gactgcttga
    10801 tttacgggtc tattttgtat attccattat taaacataac caacatgaag aaactgatcc
    10861 tccttaccgc catggccgca atggccctgg ttttgtcgaa ttgctccggg tctaaaaaac
    10921 tggccgcaac acccaagttg aattttgaat ccaacctgaa ggccgtggta atgtccgaat
    10981 gcgctccttg ccacatcccc gcaaaagggg gcaataagaa accatatgac aattacgcga
    11041 atgtaaaaac cgatatcgat gagattatcc gtcgtattga aatgaatccg ggagaaagag
    11101 gatttatgcc cttccggaaa accacgaaac taagcgatag taccattgcc ctctttaaac
    11161 aatggagagc agatggcgta ttggaaaatt aacaatcagt ctttgagtaa taactcataa
    11221 acttcacggg taaaatcggc ccggaatatg gaaaatgtcc tgatctcggc ctccggaaca
    11281 agttcaatca tcatgttatc ttcataacgg gagatatact gattctcatt gatctttttc
    11341 cagttgtata ttttcttatt cagcagtact tccaggtacc cgttgtaaca ggagtcgcag
    11401 cgggtacttg tcgtttctgt catacataac ccttttttat caaatacata gatcagttgg
    11461 gtcaggtcac tgacttgtac tagaatggag tctttccggg tataaacggc aggttgtcct
    11521 gattttccgg gatacgcttt tttcagggca gtttcggctt tgttgtaagg catgttgatg
    11581 aactcctgtg cagcagccgg gctgcaacaa gccagcaaag agaaaaagaa caataaaatg
    11641 agtttcatga aaattcagac gttatgccgc caaaggtagt tgcttggaac ggtttagaga
    11701 aacccggatt tgtcccctat atttgcgcct tccgaaaaaa ctacttatgg cattactgga
    11761 agataaaata cgtgttaaga agtccggact ccccggcgcc ggcaaaggac tttttgcgaa
    11821 atcatttata cccaaaggaa cccgtatcgt ggagtataaa ggagatatca ccacctggaa
    11881 agaagtggat catgaggatg gggataacgg gtatatttat tacgttaaaa gaaatcatgt
    11941 gatcgatgcc ttgcgaactc cggaggcatt ggcccgctat gccaatgatg cccgtggcat
    12001 tgtccgggta aagggtattt ccaataattc ggaatacatg gaagagggac tgaaagtcta
    12061 tatcacatcc gtaaaggata tacctgccgg tggggagata ctggtagatt atggtaaaga
    12121 atactgggat gtgatccgtc ataacatccg gctggaggcg aaaaaacaga aagagctggc
    12181 cgctaaaaag acaaagtcaa aaaagaagcg ggcctgattc aggatccggt aagttctcca
    12241 taataggcct gcagccgggc agctgactca cggggaaagg taatcgtatt tgggaaaaca
    12301 aatgtttcgt caggtccggg ttgcaaacgt tctgccagtt cctcccactt ttgagtccaa
    12361 acaacataat gcgctatcaa cgcttcggca tcctgcagca ggaaaccggg taagtttgct
    12421 ttatgctcgt ccagcagctg aagagctttc ctattattgg ctttcaatac ctgcgcatac
    12481 ctgtatgttt taccagcggc caggtaagct ttataatcaa tcacagattg atcaaaagtg
    12541 gcgagaagtg ttttaagaaa ttctttcata agcgcgtttc agttcaaaga taatacgcat
    12601 ttcacattat ttccggaatc cgatcctgtc tctgtcttcc catttctttt gtttcacttt
    12661 ttcatccagc aggttctcca gaacaacgta gatctgattc aaacgaccat tgtgattacc
    12721 tgttattttc tttaatgctt ttatttcatg agccagctcc tggtagttca atgcgaattc
    12781 tttcaatgaa ataaatgcac gtacaatagc gatattcatc tgaacagctt tgcctgaccg
    12841 caaaacggat gccagcatgg tgaccccgtg ttctgtaaat gcaaatggca atgacggact
    12901 atactttatt gagtcgtcga ggttatcaca atttgtgata acctcctgcc attctttttt
    12961 tgtcagaata aacataaagt ctttcggaaa gcgaagccgg tttcttttca ccgcctgttt
    13021 taatgagcgg gtctcggttc cgtacaatct tgccagatcg aaatccagca ttactttttg
    13081 ccccctgatc tcataaatga tggattgaat tttctttatc tgcataagat ttttttcagg
    13141 gtagtaaatt atcttatccg attcatcttc tctaatccgg aataacagaa aaacaaccgg
    13201 aacaacagtt ttttcctgct taccgggcat aaaaaaaccc tgtaaacagg gttttaaaac
    13261 aaggttatgt gggttagatt cctgcgagat actgaccctg tatgaatttc gccacatccg
    13321 ttaaattttt ggtttgttca aaaaccgcca gctgacggtc cgccccggtg ccatgctcca
    13381 gtatccggtg cacattggca atccggtgcc ggctgcccag gtggtccagt accggatcca
    13441 ggaaatcgag caattcata
//
LOCUS       DAOPMW010000051        13412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4692_length_13412_cov_7.667216, whole genome shotgun sequence.
ACCESSION   DAOPMW010000051 DAOPMW010000000
VERSION     DAOPMW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13412
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_13412_cov_7.667216"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLU11_04470"
     CDS             complement(<1..701)
                     /locus_tag="PLU11_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58401.1"
                     /translation="MRYFKHFMAIALLAVSMISQAQNGTGRIKGTVTDGSAKILESAT
                     ITLLRASDSSVAKISVADRNGRFEFEGVAAGRYFVRISAVGHEKGYSETVELNDTNEL
                     IVLKNIDLIPQPKSMGGVTVTAKKPLIEQRIDKTVVNVEASVTNVGTSAMEVLEKSPG
                     ISVDKDGNISLKGKQGVQVFIDGRPSYLSGTDLANYLRSLSSNQLDQIEIMTNPPAKY
                     DAAGNSGIINIKTKKT"
     gene            complement(965..1738)
                     /locus_tag="PLU11_04475"
     CDS             complement(965..1738)
                     /locus_tag="PLU11_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN58402.1"
                     /translation="MNRLFKTIVIDDEPAARRLMKSLLAEFSGSIEVIDEAGNGREAI
                     EKIHALRPDLIFLDIQMPDLTGFEVIEQLDYKPNIIFTTAYEQYAIKAFESFSIDYLL
                     KPIKEERLEQSIRKLTEFGKIAGPGPGIDVSGLQEIIRQLQAPPKATALPIKIGDRII
                     LLRFEQIAYMEAEDKYVYIHTTEGVKHLTDHSLTTLSEKLPSQFYRIQKSYIINKDRI
                     REMHRHFNGRYLFIMDDKAGTRLTSGRTYAEAIRAEFGL"
     gene            complement(1722..2882)
                     /locus_tag="PLU11_04480"
     CDS             complement(1722..2882)
                     /locus_tag="PLU11_04480"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN58403.1"
                     /translation="MQIPFWIQIIIIGGSAIMLTLAIYLMSVFNRLFTGIPNQWLRKN
                     LAVLLVAICFMFSLTAISSELFVEAAQKGWANAALFQVLNTILITFFVYNSFLLVNRI
                     KSLKQLSFVRYHAVIILTIIVSALIIYTGINYFENGMKFVKLKATLLFSFYNAATAGL
                     IYASVSYVELDRKRKLNEKELEVTRLMALKTKAELDALHSKVNPHFLYNALNSIADLS
                     VTDGKKARRMTIALADLFRYSINYSQNNFSSVNDEVAMTEVYLQIEKIRFEDQLNYTV
                     AVEPEAGHYLLPRFLLQPLVENAVKHGLKATGLMTEIHLSVNIQGEQMQISIADNGPA
                     FPDELTPGYGVKSVYDKMDLLFPGLYEIRFINQPEKQVVLTLNKLIKNEPVI"
     gene            2979..3995
                     /locus_tag="PLU11_04485"
     CDS             2979..3995
                     /locus_tag="PLU11_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPN58404.1"
                     /translation="MIIDFRSDTVTRPTPGMQEAMMKAPVGDDVFGEDPSINTLQEMT
                     ADLFGMEAALFCPSGTMTNQIAIKCHTQPGDEVICDESSHIYQYEGGGIALNSGASVK
                     LLYGDRGRINARQVEEAIQPDDVHRAHTRMVSLENTSNRGGGSCYTFSEIQAIQQVCM
                     RHDLAFHLDGARLWNAIVARKEDPLHYGKVFDSISVCLSKSLGCPVGSLLLGTKDFIK
                     KAKRYRKVFGGGMRQGGFMAAAGIYALEHHIERLKEDHANAKAIATAISSRSFVKMML
                     PVETNIIIFELDESLTAPQLVARLKEQDILGYAISPNRVRLVTHLDITPAMTEKTIQV
                     FNQL"
     gene            4000..5649
                     /gene="pgi"
                     /locus_tag="PLU11_04490"
     CDS             4000..5649
                     /gene="pgi"
                     /locus_tag="PLU11_04490"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPN58405.1"
                     /translation="MLPRINPTQTNAWKKLKAHQAEISQTNMRELFRQDSERFLKYSL
                     CTKDLVFDYSKNILNEQTLQLLEELAMSCKLPEAIQSMFNGEQINATEKRAVLHTALR
                     NFSGKPVFTQGVDVMPGIKKVLKKMKGFCEQIHSGKHRGYTNKKIKYIVNIGIGGSDL
                     GPLMVTEALKPYWMEGIETYFVSNVDGTHISETLKKVDPERTLFLVASKTFTTQETMT
                     NALTARDWFLKKAKKETYVAKHFVALSTNEKEVVKFGIDKNNMFEFWDWVGGRYSLWS
                     AIGLSIALTIGYKNFEQLLKGAHHIDEHFATSPFGKNIPVIMALTGIWYTNFFGAQSE
                     AILPYDQYLHRFAAYFQQGNMESNGKSVDRNGEPVDYATGPVIWGEPGTNGQHAFYQL
                     IHQGTPLIPCDFIAPAQSQNPIGDHHVKLLSNFFAQTEALMNGKTEAEAEAELEKQGL
                     STEEIARLLPFKIFNGNRPTNSILLKKVTPFTLGELIALYEHKIFVQGVIWNIYSFDQ
                     WGVELGKQLANKILPELKGKTPVTNHDASTNGLINSWKKMK"
     gene            5681..6058
                     /locus_tag="PLU11_04495"
     CDS             5681..6058
                     /locus_tag="PLU11_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58406.1"
                     /translation="MKVIFLSIGLLAVTSVAGQQKETIDLDELIRYRYDSSLHKMKWS
                     SEFLQKQLNPAPNPNNQPESKDLKGQLDYQLPNGDLVFRLPTDRMPCVKPGNQLLYTM
                     PVLPFKKEQIQPEKPGSIPNPAL"
     gene            complement(6609..9917)
                     /locus_tag="PLU11_04500"
     CDS             complement(6609..9917)
                     /locus_tag="PLU11_04500"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPN58407.1"
                     /translation="MQPKFTLKKRGVFYFLTPLFIAVFSLFAGTTNAQTVSTASGTNF
                     NGDYSINNPAPLVQTFVIENANATPRVFTDLSMQMAPYFTSVAGSTSNITLYYSSASL
                     SGPVTTVGAPDWIQIATGTATVPAALATVPILTGMTFLIPANTQYRFAVVASTGMAFS
                     FSPTPTPNTFTVSGVSLKVGNAQIAAQNVGYAGVFPALPSGNTPAFFGGSVTLSANFP
                     CAGTPAPGNTLSTLSTACPGTNFTLSLQNLTDGTGVTYQWQSSPDGTTWSNISGATGA
                     TYTTTLTAATYYRCNVTCSGNTTASNPLQVALTPPSGCYCTPAASDCSDDDVITRVRI
                     STLDNSSACSPNGYGNYTNTLPAPIVYSGAANPITVNVPTTWTEQVAVWIDYNQNGQF
                     EASEFTNVGSNVGNGGVITANIAIPAGLPNITTRMRVRVRFSTAWTSGLACSAITFGE
                     TEDYNVTIAPCVPVTITSNPATSVTTTCGSNASFTVAATGTLPVYGWEWRPNSTSAWQ
                     NVTNGGVYSGATTATLTLTNVTAAFNGYQYRALVSGGCSAVDFSTTSTLTVNAIQPVV
                     SPASASICLGSVQQLSLTNIVSAPTTTTFTASAGLPLAIPDATPNGVWNSVNVSGIPN
                     GSVITDVSLRLNINHTWVGDLEINLLAPNGANMNLIAELDGGTGSNGTDNFTNTVISS
                     TGTVALSGFAAPRTGTFRADRLQFYGPSVGPGGSGTGYQTVAGMDWPTLLTTINGTWN
                     LGLSDWFNGDAGTLTGWSLAITYVAPNFAQGTWAGPAGTIFTDAAATTAYTGTPATSV
                     YVLPTAAGVNNYTVSFTTPTQCTSATTTVPVTVSAPITAATVTPATRAVCLGGSTTFG
                     STVTGGTNTTYQWQRSTDAGLTWSNIGGATSATLTVSNVTSGMTGYRYRVVASSGACA
                     SFTSTNFGTLTVNALPVVTIASPVVNVVPGTTTNVTGSSTPAAAATNSWNWTLNGAPL
                     TGNTNTQTASVDALGTYQATVTDVNGCVNTSNQLVIGAEASDKLWIYPNPTPGAFQVR
                     LYYAGNTAEKRIVSIYNPKGQLITSRTFDLNYRSTPYLSMNFDLSGMARGTYVVKVAH
                     EYSGKVISGLVLVH"
     gene            complement(10107..10556)
                     /locus_tag="PLU11_04505"
     CDS             complement(10107..10556)
                     /locus_tag="PLU11_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPN58408.1"
                     /translation="MAYENNDKKMESIYSKRIRAGKRRTYFFDVRATRGNDYYLTITE
                     SRKRFDDNGYDRHKVFLYKEDFNKFIKALGEAVDYVKTELMPDFDFDAFNHEDSDEYN
                     NGIEGGTVEASTQETVSASIDEVPASSAPVAESGTESGTAAEEVDKW"
     gene            complement(10688..12532)
                     /locus_tag="PLU11_04510"
     CDS             complement(10688..12532)
                     /locus_tag="PLU11_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN58409.1"
                     /translation="MKHLSALNKYFWKYRIRLGGGILFVFLSNYFNVLSPQVTGFVID
                     FVQRSLNLPGYKAPEKKPDYDMLVDKFITSVDGSGYSIGKIVAICGMTILVLALLRGF
                     FLFLMRQTIIVMSRYIEYEQKNEVFSHYQRLDMAFYKAQSTGDLMNRIAEDVSRVRMF
                     TGPAIMYLANLISVISLSVFFMLKRDPELTLYVLAPLPILAITIYFVNTTIHRRSEKI
                     QESLSQLTTNAQETYSGIRVIKSFVQEKAMMGFFTKNAEEFKKNVLGLVKVEAIYFPS
                     ITLLIGLSTLLTIMIGGLYYIKGGHGIGVNTIVEFVMYINMLTFPVSAIGLTASMIQR
                     AAASQKRINEFLETEPAVRNEQTGEKTTLKGAIRFDQVDFVYPNTGIRAIQDFSLTIR
                     PGEKIAIVGRTGSGKTTLAQLLLRMFDVTGGCIELDGKDIRQINLADLRGQISYVPQD
                     VFLFSETVENNIGFGLTAYDHTQVEGAAGRASVDKEIEGFSERYETMIGERGVTLSGG
                     QKQRISIARALIKNPEIVIFDDCLSAVDASTEKAIISGLNEYLEAKTAIIITHRIFSL
                     FNFDRVVVLENGRITETGTHEELLAVNGYYASLYRKQQQDTEPIPPVS"
     gene            12612..12696
                     /locus_tag="PLU11_04515"
     tRNA            12612..12696
                     /locus_tag="PLU11_04515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12646..12648,aa:Ser,seq:tga)
BASE COUNT         3735 a         3003 c         3083 g         3591 t
ORIGIN      
        1 gtcttctttg ttttgatatt aattataccg ctgttgccgg ctgcatcgta tttggccgga
       61 ggattcgtca taatctcaat ctggtccagc tgattgctgc tgaggctgcg caggtaattg
      121 gccaggtcgg taccggataa ataactggga cggccatcga taaatacctg tacgccctgt
      181 tttcctttca aactgatatt accatcctta tcaacagaga tacccggcga tttttccaat
      241 acttccatgg ctgatgtacc cacattggtt accgatgcct ctacattcac gacggtctta
      301 tcaatccgtt gctcgataag cggttttttg gcagttactg taaccccgcc catggatttg
      361 ggctgtggga tcaggtcgat gttttttaac acgatcagct cattggtgtc attcagttca
      421 acagtctcag aatagccctt ttcatgccca acggctgaaa tacgtacgaa gtacctgccc
      481 gctgccactc cctcaaattc aaaccgccca ttgcggtcgg cgacactgat ttttgccaca
      541 ctcgagtcgc tggcccttaa aagggtaatg gtagccgatt ccaggatttt tgcactacca
      601 tcagttacgg tacccttaat ccggcctgtt ccgttctggg cctggcttat catcgataca
      661 gccagaaggg cgattgccat aaaatgttta aaatatctca taatcgttga ttttaatgtg
      721 tagtacctgt ctttggatat tacatcccaa aattcagtac tttgcattac atagttcatt
      781 gttttaaatt aaacggcctg tttcagggat gttcggccgt ttctaagtat ggaagtatga
      841 cggtttgagg caaaccgggt tacaaatcag cccttgtttc gtattaatgg cgatttagac
      901 ggatagacgg aatattgacc acggtcaata gtaaataagg attgggggca ggtggaagat
      961 ctctctagag cccaaattca gcccgaattg cttccgcata agtgcggccg gaggtaagcc
     1021 gggttccggc tttatcgtcc attataaaga gataacggcc attaaaatgc cggtgcatct
     1081 cacggatgcg gtccttattg ataatatacg atttctgaat gcggtaaaac tgggaaggca
     1141 gtttttcgga aagggtggta agggaatgat ccgtcaggtg cttgacacct tcagtcgtat
     1201 gtatgtaaac atatttgtcc tccgcttcca tataagcaat ctgttcaaag cgcagcagga
     1261 tgatccggtc ccctattttg atcggcaagg cggttgcttt gggtggggcc tgtaattgac
     1321 ggattatttc ctgaagtccg ctcacatcaa tgcccggacc tggtcctgca atttttccaa
     1381 actccgttaa tttccggata ctttgctcca gtcgttcttc cttgatcggt ttcaggaggt
     1441 aatcaatgga aaaactttca aaggccttga tggcatattg ctcgtaagcg gtggtgaaaa
     1501 taatattggg tttataatcg agctgttcaa ttacttcaaa accggtgagg tcgggcatct
     1561 gaatatccag aaaaataaga tccggacgga gggcgtgaat tttctcaata gcttccctcc
     1621 cgtttcccgc ctcgtcaatc acttcaatac ttccactgaa ttcagctaac aggcttttca
     1681 tgagacggcg ggcagccggc tcatcatcaa tgacaatcgt tttaaataac cggttcattc
     1741 ttgatcagtt tatttaatgt aagtaccact tgtttttccg gttgattgat aaaccgtatt
     1801 tcatagaggc cgggaaataa caggtccatt ttatcgtata cactttttac gccataaccg
     1861 ggtgtgagct catccggaaa tgcgggacca ttatccgcga tagaaatctg catttgctct
     1921 ccctgtatat ttacactcag gtgaatctcg gtcatcagtc cggtagcttt caacccatgc
     1981 ttcaccgcat tctccaccag gggttgcagg agaaaccggg gtaacaggta atgtccggcc
     2041 tctggttcta ccgccactgt gtaattgagc tgatcctcaa aacggatctt ctcaatctgc
     2101 aggtaaactt ccgtcatcgc cacttcatca tttacactgg agaaattatt ctggctgtaa
     2161 ttaatgctgt accggaacaa gtctgccagg gcaatggtca tacgccgggc ttttttgcca
     2221 tctgtaacag acagatcagc aatagaatta agtgcattgt acagaaaatg cggattcact
     2281 ttggaatgca gcgcatccag ttctgccttg gtcttcagcg ccatcaggcg ggtaacttcc
     2341 agttcttttt cattcagttt ccgcttacgg tccagttcca cataactaac cgatgcataa
     2401 atcagtccgg ccgtggccgc attataaaag ctgaaaagca acgtggcttt gagttttaca
     2461 aatttcatcc cgttttcaaa ataattgatc ccggtataga tgatcaaggc agagacaata
     2521 atcgtaagaa tgatgaccgc atgatagcgg acaaaactga gttgtttcag cgatttgatc
     2581 cggttgacca gcagaaaaga attatagaca aagaaagtga tgagtatggt attcagtacc
     2641 tggaataaag ccgcatttgc ccaacctttc tgggctgctt ccacgaagag ttcagatgaa
     2701 atggccgtca gggaaaacat aaaacaaatg gccacgagta gaacagccag gtttttcctt
     2761 agccattgat tgggtatacc ggtaaacagt ctgttgaata ccgacatcag gtaaatggcg
     2821 agggtcagca tgatggcaga ccctccgatg atgataatct ggatccagaa cggtatttgc
     2881 atgggggcaa gatattaaaa ccgggcataa aaatgagcgt ttacccgcca actaccctat
     2941 ttcaggtgtg aactgattat cttgcataaa aatttaaaat gatcattgat ttcaggtccg
     3001 atacggtcac ccgtcccacc ccgggtatgc aggaagccat gatgaaagcc ccggtggggg
     3061 atgatgtatt tggagaagat ccctccatca ataccctcca ggaaatgact gcggacctgt
     3121 ttggcatgga agcggcatta ttttgtcctt ccggcaccat gaccaaccag attgcgatca
     3181 aatgtcatac ccaacccggt gatgaagtga tttgtgatga gagctcccat atttatcagt
     3241 atgagggagg cgggattgca ttgaactcag gagcctctgt caaattatta tatggtgatc
     3301 ggggccggat taacgcccgc caggtggaag aagcgatcca gccagatgat gtacaccggg
     3361 cacatacacg tatggtatcc ctggaaaaca ccagtaaccg gggaggtggc agttgttata
     3421 cgttcagcga aatacaggcg atacaacagg tatgtatgcg gcatgacctg gcttttcacc
     3481 tcgatggagc ccgtctctgg aacgcaatcg tggccaggaa agaagatccc ctgcattatg
     3541 gtaaagtatt tgacagtatc tcagtttgcc tgagtaaaag cctgggatgt cccgtaggca
     3601 gtttattact gggcactaag gactttatca aaaaagcaaa acgataccga aaagtattcg
     3661 gaggtggcat gcggcaaggt ggttttatgg cggctgcagg tatctacgcc ctggagcatc
     3721 atatagaaag actgaaagaa gatcatgcaa atgccaaagc gattgcaact gctatttcat
     3781 cccgcagttt tgtcaaaatg atgttgccgg tggaaaccaa tatcatcatt tttgaactcg
     3841 atgaaagcct gacggccccg caactggttg cccgcctgaa agaacaggat attctgggat
     3901 atgctatttc tcccaaccgg gtaaggttag tgacacacct ggacattact ccggccatga
     3961 ccgaaaaaac aattcaagta ttcaaccaat tataaaatca tgttaccccg tatcaacccc
     4021 acacaaacca atgcctggaa aaagctgaaa gcacaccagg cggaaatcag ccagaccaat
     4081 atgcgtgaat tattccggca ggacagtgag cggtttctga aatattcact ctgtacaaaa
     4141 gaccttgttt tcgattattc aaagaatatc ctgaatgagc aaacccttca attgctcgag
     4201 gaactggcaa tgtcgtgtaa actacctgaa gctattcaat cgatgtttaa cggggagcag
     4261 atcaatgcaa ctgaaaaacg ggcagtcctg catacagccc tccgcaattt ttctggtaag
     4321 ccggtattca cacagggagt ggatgtaatg cccggcatta agaaagtact gaaaaaaatg
     4381 aaggggttct gtgagcagat ccatagcggg aagcaccggg ggtataccaa taagaaaata
     4441 aagtacattg tcaatatcgg tattggtggt agtgacctgg ggccgctgat ggtaacggag
     4501 gccctgaaac cttattggat ggaggggatt gaaacctatt ttgtatccaa tgtcgatggc
     4561 acccatattt cggaaaccct gaaaaaagtt gatcccgaac gtacactttt cctggtcgcc
     4621 tctaaaacat tcactaccca ggaaaccatg accaatgccc tgacggcccg tgactggttc
     4681 cttaaaaaag cgaaaaagga aacatatgtg gccaaacatt ttgtagccct gagcacaaat
     4741 gaaaaggaag tggtgaaatt cggaatagac aagaacaata tgtttgaatt ctgggactgg
     4801 gttggtggac gttattcact ctggagtgct attggacttt ccattgcact gaccatcggc
     4861 tataaaaatt ttgaacaact gcttaaaggg gctcatcata ttgatgaaca ttttgcgacc
     4921 tcccctttcg ggaaaaatat accggtgatc atggccctga cgggtatctg gtataccaac
     4981 ttcttcggtg ctcagtctga agcaattctg ccttatgatc aatacctgca tcgctttgcc
     5041 gcttattttc agcaaggcaa tatggaaagt aacgggaaaa gtgtggaccg gaatggcgaa
     5101 ccggttgatt atgccactgg cccggtgatc tggggtgaac ccggcacgaa cggacaacat
     5161 gctttctatc agctgatcca ccagggaacc ccgctgattc cctgtgattt tattgccccg
     5221 gcacaatcgc agaatccgat tggcgatcac catgtaaaac tgctgagtaa tttctttgcc
     5281 cagaccgaag ccctgatgaa tggaaaaaca gaagcagagg cggaagcaga gctggagaaa
     5341 caaggtttga gtacggagga aatagccaga ttactgcctt ttaaaatttt taatggcaac
     5401 cggcctacta attctattct tcttaaaaaa gtaactccat ttacactggg cgaactgatt
     5461 gccctttatg aacacaaaat ttttgtacag ggggttatct ggaatattta tagttttgat
     5521 caatggggtg tggaactggg taaacaactg gccaataaaa ttttacctga attaaaaggt
     5581 aaaacacctg ttacgaatca tgacgcttcg accaacggat tgatcaatag ttggaaaaaa
     5641 atgaaataat agctaaatta gacagcataa aacctgtttt atgaaagtaa tctttctttc
     5701 cattggcctg ctggccgtta catcagttgc tggtcagcaa aaagaaacga ttgacctgga
     5761 tgagctcatc cggtaccggt acgattcatc cctgcacaag atgaaatggt catcggaatt
     5821 tttacaaaag caattaaatc cagccccaaa cccgaacaac cagcctgaaa gcaaggacct
     5881 taaggggcaa ctggattatc aattacccaa tggcgatctg gtattccgtt taccaaccga
     5941 ccggatgccc tgtgtaaaac cagggaatca gctcctgtat accatgccgg tacttccgtt
     6001 taaaaaagaa cagatccaac ctgagaagcc gggtagtatc cccaatccgg cgttataaat
     6061 aaccgaatag gtcatatgta tctgcttagg ggatggcggc tacgccgctt ttttaacaca
     6121 tagtcacata gagacatagg agagacatag aaagcgaatg tatttctatg tctctcctat
     6181 gttattctta tagcatcatc ctgctttaga tcagttgtgt ttgtaaggat tgctgatctt
     6241 tagttgaaat tctaacccta tgtgttaaaa aattccgtga agcggatggc ggctacgccg
     6301 cttttttaac acatagttac atagagacat aggaaacaca tagtcagaaa ttggatacta
     6361 tgtgtcttct atgttatcct tttagcgtgt tccccctaaa ggtctgcggt gtttataatg
     6421 ctataagaac tttagtgaaa aattaaacct atgtgttaaa gtattccgcg tagcagatag
     6481 aggtcacggc gattcatatt gccataattc aaattcctta acgacaaaaa accagctggg
     6541 cgtcataaaa aaaaaagccc tccgttgacc ggagggcttt gtataaagtg agtggatact
     6601 acttcatctt agtgaaccag aaccagacca gagattactt taccgctgta ctcatgggct
     6661 actttcacca cataagttcc gcgggccatt ccgctaagat caaagttcat gctcaggtaa
     6721 ggcgtagacc ggtagttcag gtcaaatgta cggctggtga tcagctggcc tttcggattg
     6781 tatatggaaa caatacgctt ttcagcagta ttgcctgcat agtacaaacg tacctggaag
     6841 gcacctggag tcgggttcgg atagatccag agtttatctg aagcttctgc accgattacc
     6901 agttggttag atgtatttac acaaccgttc acatccgtta cagtagcctg gtaagttccc
     6961 agtgcatcca ctgaagccgt ttgtgtgtta gtattaccag tcagcggagc accattcagt
     7021 gtccagttcc agctgtttgt tgcagcggca gcaggtgtgc ttgaaccggt aacattggtt
     7081 gttgtacccg gaaccacatt caccacaggt gaagcgattg ttacaacagg cagtgcgttc
     7141 acagtcaagg taccaaagtt ggtagatgtg aagcttgcac aagcaccgct ggaggcaacc
     7201 actctgtagc gataaccagt cataccagaa gttacattag aaacagtcag tgttgctgaa
     7261 gtagcaccgc cgatattgct ccaggtcaga ccagcatcgg tgctgcgctg ccattgataa
     7321 gtggtattag taccgcctgt aacggttgaa ccgaatgtag tgctaccacc aagacatacg
     7381 gcacgtgtgg ccggagtaac agttgcagca gtaatcggag cgctgacagt aacagggact
     7441 gttgtagttg cagaagtaca ttgtgtcggt gtagtgaaag acacggtgta attgttcaca
     7501 cctgcggcag tcggcaacac atacactgaa gttgcaggag ttcctgtata agctgtggtt
     7561 gcagcggcat cagtaaagat cgtaccggca ggaccagccc atgttccttg tgcaaagttc
     7621 ggtgcaacat aagtaatggc gagtgaccag ccggtcaggg ttcctgcgtc accgttaaac
     7681 cagtcagaca gaccgaggtt ccaggtaccg ttaatggtag tcaggagtgt tggccagtcc
     7741 ataccggcta cagtctgata ccctgttcct gaaccaccag gtcctacaga aggtccgtag
     7801 aactggagac ggtcagccct gaaagtacca gtacgtggag cagcaaaacc actcagtgca
     7861 actgtaccag tagaactgat caccgtattg gtaaagttgt cagtaccatt actacctgta
     7921 ccaccatcca gctcagctat caggttcatg tttgcaccat tcggagcaag caggttgatt
     7981 tcgaggtcac ctacccatgt atggttgata ttaaggcgga gacttacgtc tgtaataaca
     8041 gaaccgttag gaataccact tacgttcact gaattccaaa caccattagg tgtggcatca
     8101 ggaatagcca gtggcaaacc agcagaagct gtaaaggttg ttgtagtcgg tgcagataca
     8161 atattggtta atgacagttg ctgaacacta cccagacaga tgctggcaga agcagggctc
     8221 acaacaggtt gaattgcatt aactgttaaa gtagaagttg ttgaaaaatc aacagctgaa
     8281 caaccaccgg aaactaatgc acggtactgg tagccgttga aagcagcagt aacgtttgtg
     8341 agagtcagtg ttgcagtagt tgcacctgaa taaacaccac cattggtaac attctgccag
     8401 gcagaagtac tatttggtct ccattcccat ccgtaaacag gaagtgtacc ggtagctgcc
     8461 acagtaaagc tggcattgct gccgcatgta gtggttacac ttgttgcggg attgctggta
     8521 attgtaacag gaacacaagg agcaatcgtt acattataat cttctgtttc accaaaagtg
     8581 attgcagaac aggccaggcc actggtccag gcggtgctga aacgaaccct tacccgcata
     8641 cgggtagtga tgtttggcag tccggcaggg attgcaatat tggcagtgat tactcctcca
     8701 ttacctacgt tacttccaac gttggtaaac tcagacgctt caaactgacc gttctggtta
     8761 taatcgatcc atactgcaac ttgctctgtc caggttgttg gaacatttac agtaatagga
     8821 ttcgctgcgc cagaataaac gatcggtgca ggtaatgtat tagtatagtt accatagcca
     8881 ttcgggctac aggcagatga attgtctaat gtgctgatac gaacacgggt gatcacatca
     8941 tcgtcagaac aatcagatgc agcaggcgta caataacaac ctgaaggagg tgtcagggct
     9001 acctgtaatg gattggatgc agtggtatta ccagagcagg ttacattaca acggtaataa
     9061 gtggcagctg tcagtgtagt tgtatatgtg gcaccagtag cacccgagat gttagaccag
     9121 gtggttccat caggagaaga ctgccactga taagttacac cagtaccatc tgtcagattc
     9181 tgaagagaca atgtaaagtt tgtacccgga caagccgtag ataatgtaga aagggtatta
     9241 cccggtgcag gagtaccggc acaggggaag ttggcagaaa gagttacact accgccaaaa
     9301 aatgcggggg tgttaccaga aggcaatgct ggaaatactc cggcataacc cacattctgg
     9361 gctgcgatct gtgcattacc aactttaagg cttactccgc ttactgtaaa agtattcggt
     9421 gttggtgtgg gtgaaaaaga aaatgccata cccgttgaag ctaccactgc aaaacggtat
     9481 tgggtattag cgggaattag aaaagtcata cctgttaaaa taggtacagt agccagcgcc
     9541 gcaggaaccg tagcagtacc tgtggcaatc tgaatccagt caggtgcacc cacagtagtt
     9601 acaggtccgg ataaagaagc ggaactgtaa taaagcgtga tgttagaagt agaaccggca
     9661 acagaggtga aataaggagc catctgcatg ctcagatctg tgaatactct cggagtggca
     9721 ttcgcatttt caataacgaa agtctgcacc aggggtgcag ggttgttaat ggagtaatct
     9781 ccattgaaat ttgtaccaga tgcagtagaa actgtctggg catttgtggt ccctgcaaag
     9841 agggagaaaa cagcaatgaa cagcggggtt aggaaataaa aaaccccacg ttttttaagc
     9901 gtaaattttg gttgcataga aacaatttta tgttgataag cactccttga gaaaggagaa
     9961 agtccataat ccggaactaa gttagttttt ttttgttatc tgcgaataaa aagtgaaaat
    10021 ttctgtaaaa tttacgcaga cgaaacatat ataataaaaa aagcacctgt tttttcaggt
    10081 acttttaata taaaatcgtt gatagattac catttatcta cttcttcagc tgctgttcct
    10141 gattcggtgc ctgactcggc aaccggggct gaagaggcag gaacttcatc aatggaagct
    10201 gaaactgttt cctgtgtact ggcttcaaca gtcccgcctt ctattccatt attatactca
    10261 tctgaatctt catggttgaa tgcgtcaaaa tcaaaatcgg gcatcagttc tgtcttcaca
    10321 taatctacgg cctcaccaag ggctttgata aacttattaa agtcttcctt atataagaat
    10381 accttatgac ggtcatagcc attatcatca aaccttttcc ggctttctgt aatcgtgagg
    10441 taataatcat tgccacgggt agctcttaca tcgaagaaat aagttcttct ttttccggca
    10501 cgaatccgtt tactgtagat gctctccatt ttcttatcat tgttctcgta cgccactgta
    10561 taagttttta gcagttaatg aattactttg gttttgggtg ctacaaatat agagatgttt
    10621 tcgaaacggc caaatcggca tccgactttg tttcagcgga cagtaaggtc ttttattagg
    10681 aaaaaggtca gcttaccggg gggattggtt cagtatcctg ctgttgcttc ctgtaaagag
    10741 aagcataata gccgttcacg gccagcaatt cttcatgagt gccggtttct gttattctgc
    10801 cattttccag caccactacc ctatcaaaat tgaacagtga gaaaatacgg tgggtgatta
    10861 taatggctgt ttttgcttcc aggtattcgt tgagcccact gataatggct ttttcggtac
    10921 tggcatccac ggcactcagg caatcatcaa aaatgactat ttccgggttc ttgatcaacg
    10981 ctctggcaat tgaaatccgt tgtttctggc ccccgctaag cgttactccc cgttccccga
    11041 tcatggtctc ataacgttct gaaaatccct caatttcctt gtctacactg gccctgccgg
    11101 cagccccctc cacctgggta tggtcataag cagtaagccc aaacccgata ttattttcaa
    11161 ccgtctcgct gaacagaaag acatcctgtg gcacatagct gatctgtccc cttaaatcag
    11221 cgaggttgat ttgccggata tccttcccat ccagttcaat acaccctcct gttacatcaa
    11281 acatccggag cagcaactgg gccagggtgg ttttaccact tccggtacga ccaacaatgg
    11341 caattttttc tcccggcctg atcgtaagac tgaaatcctg aatggcccgg atgcctgtat
    11401 tcgggtaaac gaaatcaacc tgatcaaatc ggatagcacc tttcagggtg gttttctcac
    11461 ccgtttgttc attcctgacc gccggttcag tttccaaaaa ttcattgatt cgtttctggg
    11521 aagcggcagc ccgctgaatc atactggccg taagcccgat agcactcacc gggaaagtga
    11581 gcatattaat gtacatcaca aattcgacga tagtattcac accgatgcca tgtcctcctt
    11641 taatataata caacccgcca atcataatag tgagcaacgt actgagtccg atgagcaatg
    11701 tgatcgaagg gaaatagatc gcctccactt ttaccagtcc aagtacattt tttttgaact
    11761 cctccgcatt ttttgtaaaa aagcccatca ttgctttttc ctgcacaaac gattttatta
    11821 cccggatccc ggaataggtt tcctgggcat tggtggtcag ttgggaaagc gactcctgga
    11881 ttttttcact tctgcggtga atggtcgtat ttacaaaata gattgtaatg gccaggattg
    11941 gtaacggggc cagcacgtag agggtgagct ccggatcccg cttcaacata aagaacacac
    12001 tgagtgaaat gaccgaaatg aggttggcca ggtacataat agccggaccg gtaaacatcc
    12061 gcacacggct gacatcctcc gcaatccggt tcatcagatc gccggtactc tgtgctttat
    12121 aaaaagccat atccagtcgc tggtaatgac tgaaaacctc atttttctgc tcatattcaa
    12181 tgtaccggct catcacaatg atcgtttggc gcatgagaaa caggaaaaat ccccggagca
    12241 gggccagtac caggatggtc atcccgcaga ttgccacaat tttcccgatc gaataccctg
    12301 atccatccac cgaagtgatg aatttatcga ccagcatatc ataatcaggc tttttttccg
    12361 gggctttata accgggaagg ttcagtgaac gttgtacaaa atcaatcaca aaaccggtta
    12421 cctgggggga taacacgttg aaatagttgg acaggaatac aaaaagaatc cctccgccga
    12481 gccggatccg gtatttccag aaatacttat tgagggcgct gaggtgcttc aattgtaatg
    12541 atttgaaaca aaattacttg aatagcgaat aatttttgga agataaagtg caagccgttt
    12601 attttgcacc cggagagatg gcagagcggt ctaatgcggc ggtcttgaaa accgttgact
    12661 gttacaggtc cgggggttcg aatccctctc tctccgcctt cgcccttcga agctaaagct
    12721 acgaagggct ttttgttgaa tttacctcct cagaatgatt tgtttgaggg ctacggcgga
    12781 taaatccgct aaataccttc gcccttcgaa gctttagcga agaagggatt cgccctccgt
    12841 agctaaagct acggagggct ttttgttgaa tcacttttct gaaaaaaatt accaagggct
    12901 tcggcggaca atcccacctt tctcttgtta ccagcggcat ttatatcata ttactattat
    12961 atttcgcccc gctggggctc agtggagctt ttctaacctt tcggctaatt gtatgtcgcc
    13021 ccgctggggc taaaggcaga ttcttcgtat tatgctgatt ctacaaataa gattgattgg
    13081 attttaactg gcaagtaata ataaactgcc tgatcgggct aaaggcagtt tctttgattt
    13141 atgtggtttc tgagcgacta aaaaccgata gatttcaacc ggctgaaata aaaaagccgg
    13201 tccattgctg gaccggcccg aatatttttc attgttaatt ttagaaagaa aggactcctt
    13261 cccggaaacc ggtcgtcttc tcggtcactt taatcgttac tttcttacgg gcctgcaggg
    13321 cctgttcaag tttttcttta atatctgtat agactgatgc cggataataa atactcacca
    13381 tccctgcttc cctggccaca gccaacggca cc
//
LOCUS       DAOPMW010000052        13360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4720_length_13360_cov_8.387073, whole genome shotgun sequence.
ACCESSION   DAOPMW010000052 DAOPMW010000000
VERSION     DAOPMW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_13360_cov_8.387073"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PLU11_04520"
     CDS             complement(<1..1289)
                     /locus_tag="PLU11_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58410.1"
                     /translation="MKRLLSFTGLLIVVLPAAGQLQNCPSNINFASGDISFWAARTGL
                     VNGGFQLYPIPNSGIRTIPEYTITGTGVEVISTQGTDPFGSFQLIPTINGYAYGHSVK
                     MGSTATSWDYRSANGNATSNPGGFTRAITYTINVPAGSPAIPFTMTYAYAMVLENGTH
                     NSNQQPLFKATLSTEDSIVTCASPQYYLPTFNDAGGGGGSTGATLDTASALANGFSLS
                     PTPFLTHTGNNNNAGTLLFDVWTKGWTEVTFDLSAYRGRQVMLTFESVNCTPGGHFAY
                     AYVAVRNTCAGLEISGSPVACANSNMTYSIPALAGAIYNWTVPAGWTIVSGANSNILN
                     VIAGTGGGQIIAHEINGCADLRDTLDVTTTLATIPGLVNSNNSVCSGTNNTLLNLTGE
                     RGTVINWISSTDGLSWNIIGNQTTSYTAQNLTSTTQY"
     gene            complement(1621..2481)
                     /gene="rfbD"
                     /locus_tag="PLU11_04525"
     CDS             complement(1621..2481)
                     /gene="rfbD"
                     /locus_tag="PLU11_04525"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPN58411.1"
                     /translation="MAELPTILVTGANGQLGKEMKELESAYPGFRFIFLSREDLPIHH
                     FELVRNFFKGYKPQYLVNCAAYTAVDKAETEKDLAFQVNGEAVGVLAAVCKEYGTKFL
                     HISTDYVFDGNGTVPYKTDAATNPQSVYGASKLKGEQEAMQFNPDSIIIRTSWVYSTF
                     GKNFVKTMRKLMSEREDLNVVNDQVGSPTYAADLAEVIMQIIAGDKWVPGMYHFSNTG
                     VISWYDFAVAIKELSGLTCRVHPIPSSQYPTPAKRPAYSVMDTATIFSTFGCTPKNWK
                     DSLQVCLQKV"
     gene            complement(2481..3041)
                     /gene="rfbC"
                     /locus_tag="PLU11_04530"
     CDS             complement(2481..3041)
                     /gene="rfbC"
                     /locus_tag="PLU11_04530"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPN58412.1"
                     /translation="MPFIDTSFPGLFIFEPRVFEDSRGYFFESFNDRIFREQGIATQW
                     VQDNQSSSCYGVIRGLHYQLPPNAQTKLVRVLRGEILDAVVDIRKGSPTFGKAFTVVL
                     SAENKKQLLVPKGFAHGFSVLSPQAEVLYKCDGFYAKESEGGIIYNDPELNINWQVPA
                     DKAIISEKDMVLPTLANCSNTFEFDA"
     gene            complement(3043..4332)
                     /locus_tag="PLU11_04535"
     CDS             complement(3043..4332)
                     /locus_tag="PLU11_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPN58413.1"
                     /translation="MYQELVDKKVKLAVIGLGYVGLPIALEFARKISVIGFDISAKRI
                     DMMKQGIDPSNELEKEAFDGCDITFTDSLDVLREAKFFIVAVPTPVDEHNVPDLTPVQ
                     KASETVGKVVKKGDYVVFESTVYPGCTEEDCLPIMEKLSGLKNIIDFKLGYSPERINP
                     GDKQHTLSSIIKVVSGCDAESLDQIAKTYELVVKAGVHRASNIKVAEAAKIIENTQRD
                     LNIALMNELSLIFDKMGINTYEVLEAAGTKWNFLKFQPGLVGGHCIGVDPYYLTHKAK
                     ELGYHSEVILAGRNINDNMGKYVAKKVIQHIIKNSGDVKSSKVLIKGATFKEDVSDIR
                     NSKVSDVVNALKEYYVNVDVEDPHADSDELKHEYGFGLVDKVANDYDAVIIPVPHKQY
                     KDLDENYYASITKPHAMVADLKGIYRNKITNRTYWSL"
     gene            complement(4351..5490)
                     /locus_tag="PLU11_04540"
     CDS             complement(4351..5490)
                     /locus_tag="PLU11_04540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPN58414.1"
                     /translation="MRQIQMVDTQTQYRKIKTEVDQAVIGVLESSAFINGKPVHSFAA
                     NLSAYLGVKHTIPCANGTDALQIAMMALGFQPGDEIITPSFTYIATTEVIALLRLTPV
                     FVEVDPKTFCIDPLALEQAITPRTKAIVPVHLYGQAAPMEEIMAIAAKHNLYVIEDNA
                     QALGADYTFSDGTKKKTGSIGHIGCTSFYPSKNLGAFGDGGAIFTNDDALADQLKMIA
                     NHGQSRRYYHDVVGCNSRLDTIQAAILDIKLTHLDEYVAARRTAADFYDQAFSGNKKI
                     IVPYRASWSHHVFHQYTLVLEGADRDALSQYLAEKQIPSMIYYPVPGHKQKMFEQFGT
                     ASQMMPVTDWLTSRVISLPIHTEMDQEQLDYITKHILEFVNHQPQ"
     gene            5588..6856
                     /locus_tag="PLU11_04545"
     CDS             5588..6856
                     /locus_tag="PLU11_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPN58415.1"
                     /translation="MAVFFYNIFLWVFKAGIHIASLFSAKASKWVQGRKGLFDKLTLA
                     IPADEKIIWMHCASLGEFEQGRPLLEKMKEQFPDYRFLLTFFSPSGYEVQKNYKGADW
                     VFYLPADGPHNAKRFLEIVHPSLVIFVKYEFWYYYLKKIKYRNIPLVLVSALFREDMS
                     FFSWYGGLQRKMLSRFDHLFVQNERSAELIKGIGLENICSVSGDTRFDRVAAIAGRFE
                     PIPLVEEFCGNSPVLVAGSTWPEDEMVLAGAFHSLRDLPLKMIIAPHETDEKNIRELK
                     KLFPEAVRFSEAKTGALHKEKVLIIDNIGMLSRLYKYGWLTYIGGGMMKNGVHNVLEA
                     AVYNKIVLFGPFYQKYTEAIGLVKSGGGITFTDEAKNGHTLKLLIETLADDESEYRMR
                     SEAAGDFVMSHRGATDKILHYIQENRLLTS"
     gene            complement(6829..7551)
                     /locus_tag="PLU11_04550"
     CDS             complement(6829..7551)
                     /locus_tag="PLU11_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58416.1"
                     /translation="MERIESLINKLKQQFDQQADPAVLLGTVQLIQLELTRSGTHSSQ
                     GLGTAKVAVMLPSRPVQSEERPTVVTAGYEKYMPATAPAAEKVVEKKETLLVEEDNVS
                     VVQKNGQLDMLFDPMTEIPTLSHQSQPRGNEVNDKTSHGESLNDRLKQGKTELMEVLK
                     ETPVRDLRKAIGINDRFIFLNELFRGDENSYERSIRTINNFNIYAEAEFWISRELKLK
                     LGWDTNSPTVLHFDQLVKRRFS"
     gene            7647..8213
                     /locus_tag="PLU11_04555"
     CDS             7647..8213
                     /locus_tag="PLU11_04555"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPN58417.1"
                     /translation="MFIEPNTSGERRGSIEVICGSMFSGKTEELIRRLKRVKIANQKV
                     EIFKPAIDIRYDEVKIVSHDAQAIHSTPIDNSQKILLMAGDADVIGIDEAQFFDPEIA
                     NVCDELAFRGIRVIVAGLDMDYLGKPFGQMPFIMAKADYVTKLHAICMKCGSIANYSY
                     RKIPNEDQVMLGATDAYEPRCRKCYHEK"
     gene            8287..8922
                     /locus_tag="PLU11_04560"
     CDS             8287..8922
                     /locus_tag="PLU11_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HPN58418.1"
                     /translation="MTLFKKDLLLELRQQYSFYGILLYIGATIFVLFMAIDGPESAVW
                     NGLFWVIQLFICINAVAKSFMQESRGRMLYFYSIASPTAFVLSKLIFNSLLMIIMSML
                     SLLLFTMFLGNPILKAGPFIGLVLLGGWSLSLVFSFLAAIAAKAQQNAAIMAVLGFPI
                     IIPQLILLMRLSGAAFSPLLSIPTSTVLMLVALDALIILLAVILFPFLWKD"
     gene            9028..9747
                     /locus_tag="PLU11_04565"
     CDS             9028..9747
                     /locus_tag="PLU11_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN58419.1"
                     /translation="MTNEKNYMHKSWWKITAVLLLIYTVIAGFLGPVPHLPLLQETIR
                     NLYFHVCMWFAMMILFTVSVVNSIRYLRSFNLKYDIYARQFAVVAIVFGILGYITGAI
                     WASYTWADPNKPAANSFSTIARDPKLIGAAIALLVYFAYLILRDSVQDMDKKARISAV
                     YNIFAYAILFPSIWILPRILPSLHPGNDGENPALDVKNDIDSGMRTVFYPAVIGWTLL
                     GVWIATLKIRFSLLKEKKMSA"
     gene            9744..9962
                     /locus_tag="PLU11_04570"
     CDS             9744..9962
                     /locus_tag="PLU11_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HPN58420.1"
                     /translation="MKIIRQLFLLLPALLLSLLTLAQSKPVEMADTMRSNGRIYVVVA
                     VALTIMAGLILYVFRLDRKLSRLEKESN"
     gene            10053..11468
                     /locus_tag="PLU11_04575"
     CDS             10053..11468
                     /locus_tag="PLU11_04575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPN58421.1"
                     /translation="MSHKYSFFGAVERSFDKAAKFTKWDKGLLDQIRQCNAVYQMRFP
                     IKRDDGTIEVIEAYRVQHSHHKTPCKGGIRFAAEVNQDEVMALAALMTYKCTIVDVPF
                     GGAKGGIRIDPKKYSVFELEKITRRYTSELIRKKFIGPGTDVPAPDYGTGEREMSWIL
                     DTYTAMHPGEIDAAGCVTGKPISQGGVRGRREATGLGVFFGIREVCNMPEIMKKLDLS
                     TGVEGKRVIVQGLGNVGYHSAKFFQDAGAKIIGLAEYEGAIWSNDGLDVDAVFQHRKK
                     TGSILNFPGANNFEKSGDALEMECDILIPAALENVIDGKNAPNVKAKLIGEAANGPLT
                     PEADEIFSKKGIIVVPDMYLNAGGVTVSYFEWLKNLSHVRYGRLEKRFTENMNTHILG
                     QIEELSGKKVKGPSRDMILHGPDEVDLVYSGLEETMINATREIMDTWQENPDIPDMRT
                     AAYVVAINKVATAYAELGIFP"
     gene            11679..12389
                     /locus_tag="PLU11_04580"
     CDS             11679..12389
                     /locus_tag="PLU11_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58422.1"
                     /translation="MKLFLTSLLGLILFSVQAQTVDEIVQKHAATMGGLDAMNKVSSY
                     KTSGTLTAQGNELPVTTQVINGKSARFEVEVMGQSIITVYDNGKGWTINPFTGATEAT
                     EISGTQLSDYKSQAFLASNLADYKNRGHQLESAGQEDVDGVKCFKLKLTHKDDGKITF
                     YFINAADFVLIKAASKREMQGEEVDVETYYSDLKEFNGVKYFMSRIQKMNGEVFSEMH
                     MDKFELNVPVDEAIFKKQ"
     gene            complement(12459..12899)
                     /locus_tag="PLU11_04585"
     CDS             complement(12459..12899)
                     /locus_tag="PLU11_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPN58423.1"
                     /translation="MIRFRFPEPSFRIKTEAGKDYIFDSFRKKWLVLTPEEWVRQNFL
                     QYLVQELKYPLSLIAVEKEFRFGEMNRRFDILIYNSEHQPWMMVECKAPEITLDQSVL
                     EQVLRYNISIPVPYLVITNGSNGFGWHKQGPVLHPLDSLPEFGE"
     gene            12992..>13360
                     /locus_tag="PLU11_04590"
     CDS             12992..>13360
                     /locus_tag="PLU11_04590"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPN58424.1"
                     /translation="MKTKEEIVHNWLPRYTGEQLENFGEYILLTNFSNYVTMFAEWNN
                     VPVIGEEKPMQCATAGNITIINFGMGSPTAATIMDLLTAVDPKAVLFLGKCGGLKKRN
                     KLGDLILPIAAIRGEGTSNDY"
BASE COUNT         3522 a         3110 c         3138 g         3590 t
ORIGIN      
        1 ctgtactgtg tggtgctggt aagattttgt gctgtataac tggtggtttg gtttccaata
       61 atgttccagc tcaatccatc agtggatgat atccagttga tcaccgttcc ccgttcgccg
      121 gttagattaa gcaaggtatt attcgtacct gaacagacac tgttattgct gttgaccagt
      181 ccgggaatag ttgccagtgt ggtagtgaca tcaagggtgt cccgcagatc cgcgcaacca
      241 ttaatctcgt gggcaatgat ttgtcccccg ccggtaccgg caatcacgtt caggatattg
      301 gaattggcac cagagacaat ggtccagcca gctggaaccg tccagttata aatcgcccct
      361 gccaatgcag gaatggaata ggtcatattg gaattggcac aggccaccgg gctgccgctg
      421 atttccagtc cggcacaggt attccgcacc gccacatagg cataagcaaa gtgacctcct
      481 ggcgtacagt taacagattc aaaagtcagc attacctgcc gtcctcggta agcagaaagg
      541 tcaaaggtta cttccgtcca tcccttggtc cacacatcaa acaaaagggt gccggcatta
      601 ttgttattgc cggtatgcgt taaaaaaggg gtcggactca atgaaaagcc attggccaat
      661 gccgatgctg tatctaatgt agccccggta gagccgcctc caccacctgc atcattgaaa
      721 gtaggcaggt aatactgtgg agaagcacag gttacgatac tgtcttcggt tgataatgtg
      781 gctttgaata gaggttgctg gttggaatta tgggtgccat tttccagtac catggcatat
      841 gcatacgtca tggtaaacgg aatagcgggt gaaccggccg gtacatttat cgtatacgtt
      901 atggcgcgcg taaagccccc aggattggag gttgcatttc cattagcaga acgataatcc
      961 catgaagtgg ccgttgatcc catttttacg gaatgaccat aagcatagcc gttaatcgtt
     1021 ggaatcagtt gaaaactacc aaaaggatca gtgccctggg tgctgatcac ttcgacccct
     1081 gtgccggtta tggtatattc gggtatggtt cggataccgg aattgggtat gggatacaac
     1141 tggaatccgc cattgaccag tccggtccgg gctgcccaaa aactgatatc tccggaagcg
     1201 aaattgatat tcgaaggaca attctgtaac tggcctgcag ccggaagtac gacaataagc
     1261 aacccggtaa aagacaggag tcttttcact gttttaagaa gggttgctat ttggcaaact
     1321 gctttcatct ggaatccggc ctttttgaaa ttcgtaaaaa agtgaaacgc ttttattaga
     1381 ttattgtttt ccccaaacct tcaggtttag agacatttct aacaaatgta ttatttccgg
     1441 gcggtcagaa agatgactct cgttaaccgg ctggattgaa tcggaaagtt cgtccaaaag
     1501 gttcccttcc atgctgaatt tcagctgaat caaatgggaa aaatacgaga aatctccact
     1561 tctacagggg gtgaatcgca gatcggaaga atggataaca gcattaacca gagtaaaaat
     1621 ctataccttt tgtaaacata cctgcaggct gtctttccag tttttgggag tgcatccgaa
     1681 ggtggaaaaa attgtagcag tatccatcac cgaataagcc ggtctttttg ccggggtggg
     1741 atattgtgaa ctggggatcg ggtgtacccg gcaagtcaaa ccactgagct ccttaatggc
     1801 cacggcaaaa tcataccagc tgattacgcc ggtattgctg aaatgataca tgcccggtac
     1861 ccatttatcc ccggcaatga tctgcatgat cacttcggcc aggtcagctg cataagtagg
     1921 agacccaacc tggtcattca ccacattcag gtcttcgcgt tcactcatca gcttccgcat
     1981 ggtcttcaca aaatttttcc cgaatgttga ataaacccag gacgtccgga tgataatgga
     2041 gtccggatta aattgcatag cttcctgttc ccctttcagt ttggaggcgc catatacgct
     2101 ttgtggattt gttgccgcat ccgttttata cgggaccgtc ccgtttccat cgaacacata
     2161 gtccgtggat atatgcaaaa actttgttcc gtactcttta cataccgcag ccagtacccc
     2221 aactgcttcc ccgtttacct ggaaagccag gtctttttcc gtttctgctt tatccaccgc
     2281 tgtataggcg gcgcagttga ccaggtattg aggtttatat cctttgaaaa aattcctgac
     2341 cagctcaaaa tgatgaatgg gcaggtcttc gcgggataaa aagataaacc ggaatccggg
     2401 ataagcggat tccagttctt tcatttcttt gccgagctga ccgttggcac cggtcaccag
     2461 tatagtaggt aattcagcca ttatgcgtca aattcaaaag tattgctgca attggccagc
     2521 gtgggtaata ccatgtcttt ttctgaaatg atggctttat ccgccggcac ctgccagttg
     2581 atattcagtt cgggatcatt ataaataata ccgccttcgc tttctttggc ataaaagcca
     2641 tcacacttgt acagcacttc tgcctgcgga ctcagcacag agaaaccatg ggcgaaccct
     2701 ttcggcacca ggagttgctt tttattctca gcagaaagta caactgtaaa cgcctttcca
     2761 aaggtggggg aacctttacg gatatccact accgcatcca gtatctcgcc acgcagaacc
     2821 ctgaccagtt tggtctgggc attgggcggt aactggtaat gcaatccacg gatcacgcca
     2881 taacaagagc tggattgatt atcctgcacc cattgggtgg cgattccctg ctcccggaaa
     2941 atccggtcat tgaaggattc aaagaaatag ccacggctgt cttcaaacac cctgggttca
     3001 aatataaaaa gtccgggaaa ggaagtatca ataaatggca tattaaaggc tccagtaagt
     3061 tcggttagta attttatttc gatagatccc tttcaggtca gccaccatgg catgcggctt
     3121 ggtgatgctg gcataataat tttcatccag gtctttatat tgtttatgcg gaacggggat
     3181 gatcacggca tcataatcat tggccacttt gtccaccagg ccaaaaccat actcatgttt
     3241 cagttcatct gaatccgcat gcgggtcttc cacatccaca ttcacataat attctttcag
     3301 tgcattgacc acatcactca cttttgaatt gcggatatca ctcacatctt ctttaaaagt
     3361 ggcgcctttg atcaggactt tagaggattt tacatccccg ctgttcttaa tgatatgctg
     3421 gatcactttc ttcgccacat attttcccat gttatcattg atattacggc cggcgagaat
     3481 cacttcggaa tgatacccga gttccttggc cttgtgggtc aggtagtagg gatccacccc
     3541 gatacagtga ccgcccacca gtccgggttg aaacttcagg aagttccatt tggtaccggc
     3601 ggcttccagt acttcatagg tattgatgcc cattttatca aagatcaggg acagttcatt
     3661 catcagggcg atatttaagt cacgctgcgt gttttcaatg atcttggcgg cttcagccac
     3721 tttaatattg gaagcacggt gcacccctgc tttcaccact aattcatagg ttttagcgat
     3781 ctggtccagg gattctgcat cgcaaccgga taccacttta atgatggagg aaagcgtatg
     3841 ctgtttatct cccggattaa tccgctcggg ggaataaccc agtttgaaat caattatatt
     3901 cttcaggccg gataacttct ccatgatcgg caggcaatcc tcttcagtac aaccgggata
     3961 aacagtggat tcaaacacca cataatctcc cttcttcacg actttaccta ctgtttcaga
     4021 ggctttctgg acaggagtca gatccggaac attgtgctca tccaccgggg tgggaacggc
     4081 gactatgaaa aatttagctt cacgtaatac atccagcgaa tcggtaaacg tgatatcaca
     4141 tccgtcaaag gcttctttct ccagttcatt gcttggatca atcccctgct tcatcatgtc
     4201 gatccttttg gcactgatat caaaaccaat cacggaaatc tttcgggcaa actccagggc
     4261 aatcggaagc cctacatagc ctaatccgat gacggccagt tttactttct tgtcaactaa
     4321 ttcctgatac attttcttaa ggtttaatga ttattgtggt tggtggttta cgaattcaag
     4381 aatgtgtttg gtaatatagt caagttgttc ctgatccatc tcggtatgga tgggtaacga
     4441 aatcacccgg ctggtcagcc agtcggttac gggcatcatc tgggaggccg taccaaactg
     4501 ttcaaacatt ttttgtttat gtccgggtac cgggtaatag atcatggagg gaatctgctt
     4561 ttcagccagg tattgtgata aggcatcacg gtctgcccct tccagtacca atgtatactg
     4621 atgaaaaaca tgatgggacc aggaggcacg gtaaggcacg ataatttttt tatttccact
     4681 gaatgcctgg tcatagaaat cagcagcggt ccggcgggca gccacatatt catccaggtg
     4741 ggtcagttta atatcgagga tagcggcttg tatggtatcc agccggctgt tacatcccac
     4801 cacatcatgg tagtaacgac ggctctggcc gtgattggca atcattttta actgatccgc
     4861 cagggcatca tcattggtaa agatggcccc gccatcccca aaagccccca ggtttttgga
     4921 gggataaaaa gacgtgcagc cgatatgacc gatactgcct gttttctttt ttgtaccatc
     4981 cgaaaaagta taatcagctc ccagggcctg ggcattatct tcaattacat acaggttatg
     5041 ctttgccgca atagccatga tttcctccat cggcgcagcc tgaccgtaaa ggtgtaccgg
     5101 cacgatcgct ttggtccggg gcgtgatcgc ctgttcgagg gccagcgggt caatgcaaaa
     5161 tgttttggga tctacttcta caaataccgg ggtcaggcgc aacagggcaa tgacttcagt
     5221 agtggcgata tacgtaaaag aaggggtgat aatttcatct cctggctgaa acccaagggc
     5281 catcatggca atctgcagtg catccgtacc attggcgcag ggaatggtat gttttacgcc
     5341 gaggtaagca gaaagattag cggcaaagga atgaaccggt ttcccgttga tgaatgcgga
     5401 gctttccaat actccgatga ccgcctgatc cacttctgtc ttgatctttc ggtactgggt
     5461 ttgggtatca accatttgaa tttgcctcat agaatggagt gttcttgctt aagtgtcgca
     5521 aaaatagtaa aatacaggta gtttgcacta ttgcctgtac ttttgttacc agttcaaata
     5581 caacaacttg gctgttttct tttacaatat tttcctctgg gtttttaaag cgggtattca
     5641 tatcgcttcc ctgttcagcg caaaggcttc caaatgggtg cagggacgga agggactgtt
     5701 tgacaaacta accctggcga ttcctgcaga tgaaaaaatt atctggatgc attgtgcctc
     5761 cctgggagaa tttgagcagg gtcgtccttt attggaaaaa atgaaagaac agtttccgga
     5821 ttaccggttt ctgctgactt ttttctctcc ttccgggtac gaagtgcaaa aaaactacaa
     5881 aggggccgac tgggtctttt acctcccggc agacggccca cataacgcca aacgcttcct
     5941 tgaaattgtc caccccagcc tggtgatctt cgtaaaatat gaattttggt actattatct
     6001 caagaaaatc aagtaccgga acatccccct ggtcctggtt tcagcccttt tccgggagga
     6061 tatgagcttt ttcagttggt atggcggatt acagcgcaaa atgctatcgc ggtttgatca
     6121 cctctttgta caaaatgagc gttctgccga actgatcaaa ggaatcgggc tggagaatat
     6181 ctgttctgtt tcaggagata cccgttttga ccgggtggcc gccattgcag gtcgttttga
     6241 acccattcca ctggtggagg aattctgcgg aaatagtccg gtactggtgg ccggaagcac
     6301 ctggccggag gatgaaatgg ttttggccgg agccttccac tcactccggg atctcccttt
     6361 gaaaatgatc attgccccgc acgaaacaga tgaaaaaaat atccgggaac tgaaaaaatt
     6421 attcccggaa gcggtgcgtt tttcggaggc aaaaacaggg gcccttcata aggaaaaagt
     6481 actgatcatt gataatatcg ggatgttatc ccgcttgtat aaatatggct ggctgaccta
     6541 tatcgggggg ggcatgatga aaaacggggt gcataatgtg ctggaagccg ccgtatacaa
     6601 taaaattgtt ttattcggac cattttacca gaaatacacg gaagccatcg gtctggtaaa
     6661 aagtgggggt ggtattacct tcactgatga agcaaagaat ggtcataccc tgaaactcct
     6721 gatcgaaacc ctggcggatg atgaatcgga ataccggatg cggagcgaag cagcgggtga
     6781 ttttgtaatg tcacaccggg gtgcgacaga taaaatcctg cattatattc aggaaaaccg
     6841 tcttttaacg agctgatcaa aatgtaatac cgtaggacta ttggtatccc agccaagttt
     6901 caatttaagt tcccggctga tccagaactc ggcttcggca taaatattaa aattattgat
     6961 cgtgcggata ctccgttcgt agctgttttc atccccccgg aataattcgt tcaggaagat
     7021 aaaccggtcg ttgattccga tggcttttcg cagatctctc accggggtct ccttcaggac
     7081 ttccatcagt tccgtcttac cttgtttcag ccggtcattc agcgactcgc cgtgggatgt
     7141 tttatcattg acttcatttc cacgcggctg ggactgatgg gacagggtcg gaatctcggt
     7201 catgggatca aacaacatat ccagttgtcc gtttttctgc accacagaca cattgtcttc
     7261 ttccacgagc agggtctcct ttttctccac cactttttct gccgcaggtg ccgttgccgg
     7321 catatacttt tcataaccag ctgttaccac tgttggtctt tcctccgatt gtaccggacg
     7381 ggagggcaac atcacggcca ctttagcggt accaagcccc tggctgctat gggtgccgga
     7441 tcgggtcagt tccagctgga tgagctgaac cgtccccagt aaaacagccg gatccgcctg
     7501 ctggtcgaac tgttgtttta atttattgat gagcgactct attcgttcca ttcatttata
     7561 tttggcgccg gtaaaaccgg ctcaatgaag ttacaaacag tttgccaata aacccatata
     7621 cccaaaccca cccctaaatc ttaacaatgt ttattgaacc caataccagt ggagaaaggc
     7681 gaggcagtat tgaagtaatc tgcggatcga tgttcagtgg taaaacagaa gaactgatcc
     7741 gccgccttaa gcgggtaaag atcgccaacc agaaagtgga gattttcaag ccggccattg
     7801 atataaggta tgatgaagtg aagattgttt cccatgatgc acaggccatt cactccaccc
     7861 cgatcgataa ttcacagaaa atcctgctga tggccggtga tgcggatgtg atcggcattg
     7921 atgaagcgca gttttttgat ccggaaatcg ccaatgtctg tgatgaactg gctttccggg
     7981 gtatccgggt gatagtagcc gggctggata tggattacct gggcaaaccc tttggccaga
     8041 tgccctttat catggccaaa gcggattatg tgaccaaact gcacgccata tgtatgaaat
     8101 gcggcagtat tgccaattat tcataccgta aaatcccgaa tgaagaccag gtgatgttgg
     8161 gggccacgga tgcttatgag ccccgctgcc ggaaatgtta tcatgaaaaa taatagtccg
     8221 tgcttcgcca cctgaatgtt tacatttgca cgcaaataaa attctatcag ccaattccga
     8281 catatactga cccttttcaa aaaagacctg ttgcttgaac tccggcagca atacagcttt
     8341 tatggcatcc tgttgtatat cggagccacc atctttgtgt tgtttatggc tattgatggg
     8401 ccggaaagcg cggtgtggaa cggattattc tgggtgattc agctatttat ttgtattaat
     8461 gcggtagcca aaagttttat gcaggaaagc agggggcgta tgctttattt ttattcgatt
     8521 gccagtccta ccgcctttgt cttatccaag ctgatcttca actcgctgct tatgatcatc
     8581 atgagtatgc tgagtttgct gctgtttacc atgtttctcg gtaacccgat tttaaaagcc
     8641 ggtccgttta tcggactggt cttgttaggc ggctggagtc tgagcctggt attcagtttc
     8701 ctggcggcta ttgccgcaaa agcacaacaa aatgccgcca ttatggctgt gctgggtttt
     8761 ccaatcatta tcccccaact gatcctgtta atgcgtttgt caggcgccgc cttctcccct
     8821 ttactaagta ttcctacgag tacggtactc atgctcgttg ccctggatgc cctgatcatt
     8881 ttgctcgcgg taatcctttt tccttttctt tggaaggatt gatttttaac ggattccggc
     8941 ctgattatta tcaaaagaaa ttttcgcttc gtttagcagg agcaccttaa ttttgcctca
     9001 ctttaccggt cttaacagac ccccataatg accaacgaaa aaaactatat gcacaagtcc
     9061 tggtggaaga tcactgcagt attactgctg atttacacgg ttattgccgg cttcctggga
     9121 cctgtccccc acctgccctt gttgcaggaa accatccgta acctctattt tcacgtatgc
     9181 atgtggtttg ccatgatgat cctgtttacg gtatctgtgg tcaattccat ccgatacctg
     9241 cggtctttca acctgaaata tgatatttat gcccggcagt tcgcggtggt ggcgattgta
     9301 ttcggcattt tagggtatat caccggggcg atctgggcct cctatacctg ggcggatccg
     9361 aataaaccgg cggccaattc attcagcaca atcgcccggg atcccaagct gatcggtgcg
     9421 gctattgcgt tactggtata ttttgcctac cttattctac gcgattcagt acaggatatg
     9481 gataagaaag caaggatcag tgcggtatat aacatatttg cctatgcgat tttattccct
     9541 tcaatctgga ttcttcccag gatcctgccc agtctgcacc cgggcaatga tggggaaaat
     9601 ccggcactgg atgtaaagaa tgatattgac agcggtatgc gtacggtttt ttatccggct
     9661 gtcatcggct ggaccctcct tggggtatgg attgccactt tgaagatccg cttcagttta
     9721 ttgaaagaaa aaaagatgtc cgcatgaaaa taatcagaca gttattcctg ctcctcccgg
     9781 ccctgctcct ttccctgctc acgctggctc agagcaaacc ggttgaaatg gctgacacaa
     9841 tgcgcagcaa tggccggatt tatgtggtag tggccgtggc ccttaccata atggccggac
     9901 tcatccttta tgtattcaga ctggaccgca aactgtcccg cctcgaaaaa gaatccaact
     9961 aactatttac cggtcagcaa aacagaatga ttcgctggcc gtttatgttt ttatataata
    10021 acgaacgatt gctataacct aaaaaagaaa ccatgtcaca caagtacagt ttttttggcg
    10081 cggtggaaag aagtttcgac aaagcagcca aattcaccaa atgggacaaa ggcctgctgg
    10141 atcagatccg ccagtgcaat gctgtttacc aaatgcgctt cccgatcaaa agagatgacg
    10201 gaaccattga agtcattgaa gcctaccgcg ttcaacactc ccatcacaaa accccttgta
    10261 aaggaggtat ccgttttgcc gcagaagtaa accaggatga agtaatggcc ctggccgccc
    10321 tcatgaccta taaatgcacg attgtggatg tgccctttgg cggagcaaag ggcggtatcc
    10381 ggatcgatcc gaaaaaatat tcagtttttg aactggaaaa aatcacccgc cgttacacgt
    10441 ctgaactgat ccgcaaaaaa ttcatcgggc ccggtacgga tgtacctgct cctgattacg
    10501 gaaccggtga gcgcgaaatg tcctggatcc tggataccta tactgccatg catcccggag
    10561 agatcgatgc ggctggttgt gtgacgggta aaccgatttc acaaggtggg gttcggggac
    10621 gtcgtgaagc tacagggttg ggcgttttct ttggtatccg ggaagtttgt aatatgccgg
    10681 agatcatgaa aaagctggat ttgtctaccg gagtggaagg gaaaagagtg attgtacagg
    10741 gactgggtaa tgtgggttac cactctgcga aattcttcca ggatgccggt gcaaaaatca
    10801 taggcctcgc tgaatatgaa ggcgcgatct ggagtaatga tggactggat gtggatgcag
    10861 tattccagca ccggaaaaaa acaggatcga tcctgaattt cccgggagcc aacaactttg
    10921 aaaaatcggg tgatgcgctc gagatggaat gcgatatcct gattccagcc gcacttgaaa
    10981 atgtaattga tggcaaaaac gctcccaatg taaaagctaa actgatcgga gaagccgcca
    11041 atggccccct gacacctgag gctgatgaaa tattttccaa gaaaggaatt atcgtagttc
    11101 ctgatatgta cctgaatgct ggtggggtta ctgtttccta tttcgagtgg ctgaaaaacc
    11161 tgagccatgt acgctatggc cgcctggaaa aacgcttcac ggaaaatatg aacacacata
    11221 ttcttggaca gattgaagaa ctgagtggca agaaagtaaa aggtccgtcc cgcgatatga
    11281 ttttacatgg tccggatgaa gtggacctgg tgtatagcgg actggaagaa acgatgatta
    11341 atgcaacccg cgaaatcatg gatacctggc aggaaaatcc ggatatccct gacatgcgta
    11401 cagccgctta tgtggttgcc atcaataaag tggccaccgc ctatgccgaa ctgggtatct
    11461 tcccttaaac ccaaaaacca attgcttata ccatcccgtt tgcctggcag acgggatttt
    11521 ttttggaaga aattaaacta actccttatc aaccgggcca ccagctagaa acgaatgggt
    11581 cgttccaatg cagtttactc caaaaaaaat accagtcact ccaaatgctc cgtcctcatt
    11641 tttaaaaaaa ctaccttcac cgcatcaaaa aacaaaccat gaaactattc cttacttctt
    11701 tacttggact tatcctgttt tctgtacagg cacaaactgt tgatgaaatc gtacagaagc
    11761 atgccgctac gatgggcggt ctcgatgcca tgaacaaagt cagttcctat aaaaccagcg
    11821 gcaccttgac ggcccagggc aatgaactcc cggtgaccac ccaggtcatc aatggaaaat
    11881 ccgcccgctt tgaagtggag gtgatgggac aatccatcat tacggtctat gacaatggaa
    11941 aaggctggac cattaacccg tttaccggag ccacagaagc tacagaaatt tccggtaccc
    12001 agctgtcgga ctataaaagc caggcatttc tggcgagcaa cctggcggac tataaaaacc
    12061 gggggcacca actggaatcc gcgggacaag aggatgtgga tggggtaaag tgttttaaac
    12121 tgaaactcac ccataaggac gacgggaaaa tcacattcta ctttattaat gctgccgatt
    12181 ttgtactgat caaagcagcc tctaaaaggg aaatgcaggg ggaggaagtg gacgtggaaa
    12241 cttattattc agacctgaaa gaattcaacg gcgttaagta ctttatgagc cgcattcaga
    12301 aaatgaacgg agaagtattt tctgaaatgc atatggacaa attcgaactg aatgtgccgg
    12361 ttgatgaagc catttttaaa aagcaataat attatctgat agaaaagggc tgtttcataa
    12421 atgaaacagc ccttttctat gcccccgggt gaagcgtgtt attctccaaa ttcaggcaaa
    12481 ctatccaacg gatggagcac cggcccctgt ttgtgccagc caaatccatt agaaccattc
    12541 gtaatcacca ggtaaggtac tggtatactg atattatacc ggagcacttg ttccagaacc
    12601 gattgatcaa gcgtgatttc aggcgcttta cattccacca tcatccaggg ctggtgttct
    12661 gagttgtata taaggatatc aaagcgccgg ttcatctcac caaaacggaa ttccttttcc
    12721 acagcaatca gggataaggg atacttcaac tcctgcacga gatattgaag gaagttttga
    12781 cgcacccatt cttccggtgt gagtaccagc cattttttac ggaatgaatc aaaaatgtaa
    12841 tccttcccgg cctcggtttt tattcgaaac gagggttcag gaaaccgaaa acgtatcatt
    12901 gttcaaatgt aatgggtttt tcacagttag ccggccacag gtacacttcc gggaaaacga
    12961 cagagtccgt ttatttgttt aaattcgcta tatgaagaca aaagaggaaa tcgtacacaa
    13021 ctggctgccc cgttataccg gggagcaatt agaaaatttt ggcgagtaca ttctcctgac
    13081 caacttcagc aactatgtaa ccatgtttgc agaatggaac aatgtaccgg tgattggtga
    13141 ggaaaaacct atgcaatgtg ccaccgctgg aaatatcacc atcataaatt ttggcatggg
    13201 tagtccgact gccgccacga tcatggatct gctgacagcg gtcgacccca aggccgtttt
    13261 atttcttggc aaatgcgggg ggctgaaaaa aagaaataaa ctcggggacc tcatcctgcc
    13321 gattgcggcg ataagaggtg agggtacatc caatgactac
//
LOCUS       DAOPMW010000053        13245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4781_length_13245_cov_10.415315, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000053 DAOPMW010000000
VERSION     DAOPMW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4781_length_13245_cov_10.415315"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLU11_04595"
     CDS             complement(<1..995)
                     /locus_tag="PLU11_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58425.1"
                     /translation="MNTQSPHTFHIPVMGLAYTIDSPVKVARYGISSVISIVEDRLVE
                     MMRSYYYPQTGRTYLPITTDQPDYRARRITDYLNLVNTIVREQVEKIKIAAFEKGSEI
                     VTYFEMLPEQSVLKQLYRQMTQTEDEAEKRRLEAYLRNQIKPGSIDVNIMTKLDRENF
                     DANSQVVKDGSDAVASLRGYVNSDLDGSSIVFSAGMNPRLFNYMEQCSQFEMREDGSF
                     SKKVVLKVSDFRSALIQGKYLAKKGIWVSEFRIESGLNCGGHAFATDGFLLGPILEDF
                     KSGKQELLESLFEIYSAAQLKKNNRTITAIPPVLFSVQGGIGTAAEDQFLHHHYE"
     gene            complement(1175..1606)
                     /locus_tag="PLU11_04600"
     CDS             complement(1175..1606)
                     /locus_tag="PLU11_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN58426.1"
                     /translation="MLSLTCKTAIKAVIYLAARFESGEKSGMKEIAEVIEASEHTVGK
                     IMQILVKEDVIKSLKGPSGGFFITARQKSQPVMNVVDAIDGKELFKQCGLGLSKCSSS
                     HPCPIHDEYKVVRDLFENMCRKNTINDLCKPVNKGLAYLIG"
     gene            complement(1609..1932)
                     /locus_tag="PLU11_04605"
     CDS             complement(1609..1932)
                     /locus_tag="PLU11_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HPN58427.1"
                     /translation="MNIISNHPINATIALAALEQVMDPEIGLNVVDLGLIYQLDFDKQ
                     GRRIYVSMTLTTQFCPMGDTLTAAVKRVMERTFGDQEIVVELCFDPPWSQERISEQGK
                     LFLNQ"
     gene            complement(1929..3209)
                     /locus_tag="PLU11_04610"
     CDS             complement(1929..3209)
                     /locus_tag="PLU11_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HPN58428.1"
                     /translation="MSNGYPNAGFRNTPDTAVVPFYLYAAISFLACTILLSLSTESFT
                     QHYFSPQLLAITHGMALGWGTMIILGASHQLIPVLTEKSLFSIRLAHLSFFLAAVGIP
                     LLVYGFYTFNMGWPSKWGGRLVLLAVMAYLVNILYTVKTGKSGNIYSLFIVTAASWLF
                     ITAFLGLVLVYNFNFPFLPKDVLTYLPLHAHAGILGWFLMLIIGVASRLIPMFLISKY
                     QNNRRLQLIFLMINGGLIYYLLSYYFQFPYPVLFSGLMIGTALSFFLHYIFRVWQSRI
                     RKKVDQPMRISLLSVAIMLLPLFILLLVSGYRITGGQHSRLVLLYGFLIFFGWMTSLI
                     LGMTFKTLPFIIWNKHYHDKAGLMKTPDPKTLMSDRLFMAMGISYLAGFLLFALGIFG
                     GYTSLLKAGSIVMILAAVFYNLNVLKIWRHKPLV"
     gene            complement(3211..3768)
                     /locus_tag="PLU11_04615"
     CDS             complement(3211..3768)
                     /locus_tag="PLU11_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HPN58429.1"
                     /translation="MSDKPELIGALRPEQLVQLDVRPVLAGGTDPFQLIMEKINELKE
                     GQALEIINTFTPAPLITVLKRQGFSAYTEMVNDQLVHTFFYKDPAAQGPVTLANESIR
                     SHWDEINARFADNLVFADVRGLEMPEPMIRILAAVDTLPEGKALFVYHKKLPVFLLPE
                     LAERKFDYRVNEIAPGEVHLLIFRP"
     gene            complement(3789..4748)
                     /gene="ric"
                     /locus_tag="PLU11_04620"
     CDS             complement(3789..4748)
                     /gene="ric"
                     /locus_tag="PLU11_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPN58430.1"
                     /translation="MQTLTDNILNVTLLEPRLKHPTIFRYFDELTEGESLTIHNDHDP
                     KPLYYQLLGERGNIFTWEYLEEGPEWWKVRISKRKSGEAEETLGQLVAKDMRKAQIFK
                     KYGLDFCCGGKKTVKEACQEKGLDVTKVERELQLSEKEEAIRNPLPYNDWSPAFLCDF
                     IVNTHHSYVRNNLPDIVAYADKVRKVHGGRHPELLEIWQRVSEVNTEMMAHMVKEEKV
                     LFPYIHELVKVADKTESAHAKELGTVQQPIQMMEMEHEVVGEHLAKIRELTGHYTLPD
                     DACASYSLLYRMLDAFEDDLHLHVHLENNILFPKAIELEKRLN"
     gene            complement(4890..5321)
                     /locus_tag="PLU11_04625"
     CDS             complement(4890..5321)
                     /locus_tag="PLU11_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPN58431.1"
                     /translation="MNEKIAEFISKQTCGNLACVDELGNSWCFSFFYSVDVANDLLYY
                     KSSNDTRHSVILHANPKVSGTILPDKLNFLAIKGVQFEGVILPAADPLAAHAAAHYHK
                     KHPVALAMPGAIWTIRLDHVKFTDNTLGIGNKINWNRHAAV"
     gene            complement(5318..5539)
                     /locus_tag="PLU11_04630"
     CDS             complement(5318..5539)
                     /locus_tag="PLU11_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPN58432.1"
                     /translation="MPKVIHYRDKCIGCGVCYEQQSEMWRMSKKDGKATLLKGIKKKE
                     VYVLEIPASALDKSREIALACPVRVIKIS"
     gene            complement(5532..6779)
                     /locus_tag="PLU11_04635"
     CDS             complement(5532..6779)
                     /locus_tag="PLU11_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPN58433.1"
                     /translation="MRKDKVELLAPAGSFDSLQAAINAGADAIYFGVEQLNMRTRSSQ
                     SFHISDIKTVSAICRKQGLKAYITLNTVMYEYDMQLLKTVLKEVKKHKIDAVIAADFA
                     VIDYCNQMEIPLHISTQANISNVESVRFFSRYADVMVLARELTLKQVADISSEIERKK
                     IKGPSGKLVKLELFVHGALCMAISGKCYMSLHTQNAAANRGACVQNCRRPYTVTDTET
                     GEQLEIDNEYIMSPKDLCTIDILDQLIDSGVDVLKIEGRTKGADYVQVVTKCYRAALD
                     AVLDGTYTLEKAAALKKEMETVYNRGFWEGYYLGRKLGEWTAQPGSAATEKKIYIGKG
                     SKYYPKISIGEFILESGSLRIGDTMMVTGPKCGMIKETISTLMVNGEPATEAVKGDKI
                     TLKFDTKVTPSDKLYKIVEAAHA"
     gene            complement(6789..7778)
                     /locus_tag="PLU11_04640"
     CDS             complement(6789..7778)
                     /locus_tag="PLU11_04640"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPN58434.1"
                     /translation="MTLQSTYMGLKLNSPIVVSACTLSEQLDNIVKMEDNGAGAVVMF
                     SLFEEQIRKEEARMNTVMSETSYAFPEALDYFPDLDDFNVGVDEYLENIRKAKERVNI
                     PIIGSLNGITTEGWIDYSKQIEQAGADGLEVNIFFIPADITMSSSEVEHRYLNIIEAI
                     KSNVKIPVAVKLNPYFSATGNMAMRMKNSGADALVLFNRFYQPDFDINELVLKTDLHY
                     SEPSEIRLPLLWIALLFEKVKLSLAATTGVQSAVEVVKYLLAGADVVMTASSLYKNGI
                     PYLKTMNKELQDWMYMMGFEDITSFRGSMSQQNVSDPTAFERANYIRILEGMK"
     gene            complement(7875..11450)
                     /gene="nifJ"
                     /locus_tag="PLU11_04645"
     CDS             complement(7875..11450)
                     /gene="nifJ"
                     /locus_tag="PLU11_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPN58435.1"
                     /translation="MSNKNKSVATIDGNEAAAYVAYRINEVCAIYPITPSSNMAELAD
                     EWAAKGTKNIWGNVPDVIEMQSEGGAAGTVHGALQTGSLTTTFTASQGLMLMLPNMYK
                     IAGELTATVFHVAARSLAAQGLSIFGDHQDVMAARTTGFAMLGSASVQEAHDFALISQ
                     VASLKSRIPFVHFFDGFRTSHEVNKLELLSDDQIRSMVPDDLVIAHRGRGLNPDHPFI
                     RGTAQNPDVYFQGRETVNTFYNNTPGIVQDVMDDFAKMTGRKYQLFEYSGAADAERVV
                     IVMGSGGETVIETVKALQKAGEKVGVLHVRLFRPFSMDHFVKAIPASTKIIAVLDRTK
                     ESGAAGEPMYQDVLASMVEAFQLGKIKTLPRIVGGRYGLSSKEFTPAMVKAVYDELKK
                     DNPKNGFTVGINDDVTFTSLEFDPEYKLDESAWRQGLFFGLGADGTVGANKNTIKIIG
                     ENTDLFAQGYFVYDSKKSGARTVSHLRFGPEPIRAPYLISKADFIACHQFNFVEKVEM
                     LEFASTGATFLLNSPFDKDEVWSKLPGQVQQQIIDKKITVYVIDATTVARETGMSGRI
                     NTIMQTCYFALSGVLPKDDAIKQIKKAIEKTYFKKGPKVIEQNFKAVDATLAHLYQVE
                     IPAKASSAMVELLTVSDKAPAFVQDVTAMMMSGHGDDIPVSKMTIDGTYPSGTTQWEK
                     RNVSDLVPLWEPDLCIQCGNCAYVCPHSCIRAKFYHENHLEKAPEGFKSAPINARGFP
                     ETKYTLQVYIEDCTGCNLCVEVCPATEPTNRARKAINLVDKDPILKQEQKSIEFFENV
                     PWNDRSKIDFSTVHGAQFLEPLFEFSGACAGCGETPYLKLLSQLFGDRLLVANATGCS
                     SIYGGNLPTTPWAVNKEGRGPAWSNSLFEDNAEFGLGMRITADKQLSVAHQYLDELKG
                     ELGEGYVKDIVEAKQTLESELVEQRVRVDDLMEKLRSIDKPAAKHLLSVADQLVRRSV
                     WLIGGDGWAYDIGYGGLDHALASGRNINVLVLDTEVYSNTGGQSSKSTPTAATAKFAA
                     GGKAGGKKDLAMQAISYGNVYVARVAFGANPQQALLAMREAEAYDGPSLILAYSHCIA
                     HGYDLKNGLDQQQKAVASGHWPLLRYNPMLRKKNQNPFVLDSTRPTITLKDYTYNELR
                     YKVLTMTNPEVAEHLMNHAQDLVNLKWKTYEELATKKASDFVPIG"
     gene            complement(11440..13086)
                     /locus_tag="PLU11_04650"
     CDS             complement(11440..13086)
                     /locus_tag="PLU11_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPN58436.1"
                     /translation="MAANDITKPVDLLTHAEGTGPLRSRRPVYVDFMPPCNSACPAGQ
                     NIQGWLSHAQSGNYFEAFQTLLEDCPFPAIMGRVCVKPCETGCNRNHIETTVNIHAVE
                     RYIGDEAISQKWPVRFEAVPTGKRVLVVGAGPGGLSAAYHLARLGHTVEVYDAGKHPG
                     GLMWTGIPDYRLPKEILDAEVDRIVKMGVKIRLNYKVQDVLTEKQAGNFDAVYLSIGA
                     QLIRPETFPQDGSVHMTDAFEFFREAKLNSSPYVKKKVVVYGGGKLALYLSRMIKRFG
                     SEVSVYFPGDKKMMPAYDYETEDALAEGVDVQLFRSISKIERNKITLEKTKVEKGKVV
                     GTGEFETVDADVLINANKQESDTGFLRSATGIKVNDDGTVGIDGLRMTGHEGIFAGGD
                     MLPGENRSTTIAIGHGKKAAKYMHGFLMQDPYQKPDKHPTAGFRKLHMWYKTDAPQKE
                     QDKLAPAVAVKSFDEVISGLSEKEARFEAQRCLSCGNCFECDGCFGACPEDAIIKLGK
                     GNRYKFNYNACTGCAVCYEQCPCHAIDMISEPVNSTTDVK"
BASE COUNT         3501 a         3309 c         2784 g         3651 t
ORIGIN      
        1 atctcgtagt ggtgatgaag aaactgatct tctgccgctg taccaatacc tccctgtacg
       61 gaaaacagta ccggcggaat ggctgttatg gtccggttat ttttctttaa ttgagccgca
      121 ctgtatatct caaataaaga ctccaggagt tcctgcttac ccgatttaaa atcttccaga
      181 atcggtccga gcaggaagcc atccgtggca aacgcgtgtc cgccacaatt caaccccgat
      241 tcaatcctga actcacttac ccatattccc tttttggcca ggtacttgcc ctggatcaaa
      301 gcggaacgga aatcacttac tttcagtact acttttttgg aaaaactacc atcctcccgc
      361 atttcaaact gactgcattg ctccatataa ttaaataacc tgggattcat acctgcggaa
      421 aaaacaatgg acgacccgtc aagatcactg ttaacataac cacgcagcga ggctacggca
      481 tccgaaccat ctttgaccac ctggctgttt gcatcaaaat tttcccgatc cagtttggtc
      541 atgatattca catcaatact cccgggcttg atctggttgc ggaggtatgc ttccagtcga
      601 cgtttttctg cttcgtcttc ggtctgcgtc atctgacggt ataattgttt caatacagat
      661 tgctccggca gcatttcaaa ataggtaacg atctcggatc ctttttcaaa ggcggctatt
      721 ttaattttct ccacctgttc ccgtactatg gtattaacca gatttaaata atcagtaatc
      781 cgacgggccc ggtaatccgg ctgatccgtg gtaatgggaa gataggttct ccctgtttgc
      841 ggataatagt aactccgcat catttccacc aggcggtctt caacaatgga tattactgaa
      901 ctgatgccgt atcgggctac tttaaccgga ctatcaattg tataggccaa tcccattaca
      961 ggaatatgaa aagtatgggg tgattgtgta ttcatgctca ttttaaaatt caggatgaga
     1021 agctgccttt cttaaacaaa agtcagctgg taaacaggtg aacaaagaag cggtgaaact
     1081 aaagcaatag caaatcaaaa aacctgatta aactcagcaa aattgaaaaa atgaaagatt
     1141 cccttttgta aaacacgatc agttgtacag ttttttagcc gatcaggtag gctaagccct
     1201 tattaactgg tttgcataaa tcatttatgg tgtttttccg gcacatgttc tcaaataagt
     1261 cacggactac tttatactca tcatgaattg gacacggatg actggatgaa cacttactca
     1321 acccaagacc acattgtttg aacaattctt ttccgtcaat ggcgtcaaca acattcatca
     1381 ctggttggct cttctgcctg gcggttataa aaaaaccacc actcggtcct ttaaggcttt
     1441 ttatcacatc ctctttcacc agtatttgca ttatttttcc cacagtatgt tcactggcct
     1501 caatgacttc cgcaatttcc ttcataccgg atttttctcc tgactcaaac cgggccgcca
     1561 gatagattac cgctttaata gccgttttac aggtcagact cagcatactt attgatttaa
     1621 aaacaatttg ccttgttccg atatccgttc ctgcgaccag ggcggatcaa aacacaattc
     1681 aacaacaatt tcctgatcgc caaaagtacg ttccattacc cgttttacag cggctgtcaa
     1741 cgtatcaccc atcgggcaga attgagtagt caatgtcatc gacacataaa ttctcctgcc
     1801 ctgcttatca aagtccagtt gataaatcaa cccaaggtcc actacattca gcccgatttc
     1861 cggatccatt acctgttcca gggccgccag tgcaatagtg gcattgatcg gatgattact
     1921 tatgatattc ataccaatgg tttatgccgc cagattttca gcacattcag gttatagaaa
     1981 acagcggcca atatcattac tattgaccca gcctttaaca agctggtata cccgccgaaa
     2041 ataccaaggg caaagagcag aaagccggca agatatgaaa ttcccattgc cataaataac
     2101 cggtcagaca tcagggtttt cggatcaggt gtcttcatca gaccggcttt atcatgataa
     2161 tgtttattcc agattataaa cgggagtgtt ttaaaggtca tacccagaat caaagaggtc
     2221 atccagccga aaaaaattaa gaacccgtaa agcagcacca aacggctatg ctgtccgccg
     2281 gtaatccgat agccagaaac cagcaataga ataaataatg gcaacagcat tatggctacc
     2341 gataaaagag aaatccgcat cggttggtcc actttcttac ggatacgcga ctgccatacc
     2401 cggaaaatgt aatggaggaa aaaagaaagt gccgtcccaa tcatgagtcc cgaaaataag
     2461 accggatatg gaaactgaaa ataataagaa agaagatagt agatcaggcc tccattaatc
     2521 attaaaaata taagctgtaa cctccggttg ttctggtatt tagatatcag gaacatggga
     2581 atcagtctgg aggcaacgcc aattatcagc attaaaaacc agcccaggat acctgcatgt
     2641 gcatgtaaag ggagataggt cagtacatct tttggcagaa atggaaaatt aaaattatag
     2701 accagtacca atcccaggaa agccgtaata aaaagccagc tggcagcagt aacaataaac
     2761 aaagaataga tattgcctga cttcccggtc tttaccgtgt ataaaatatt aaccagataa
     2821 gccatgactg ctaaaagaac cagtcgccca ccccattttg atggccagcc catattgaaa
     2881 gtataaaatc catatactaa caacggaatt ccaactgcag caaggaagaa cgagagatgt
     2941 gccagcctta tactgaataa tgacttttca gtcagcaccg gtatcagctg gtgactggcc
     3001 cccaggataa tcatggtacc ccaacccaat gccattccat gagtgattgc caggagctgc
     3061 ggactgaaat aatgctgcgt aaaggattcc gttgacagcg ataacagtat cgtacatgcc
     3121 agaaaactga tggccgcata cagatagaac ggaacaaccg ctgtatccgg ggtattcctg
     3181 aaaccggcat tcggatatcc gttactcata tcagggcctg aagattaaca agtgtacttc
     3241 tccgggtgct atttcattca cccggtaatc aaatttacgt tcggccagtt cgggcaacag
     3301 aaataccggt aattttttgt gatatacaaa aagtgctttc ccttctggca atgtgtcgac
     3361 tgctgccagt atccggatca tcggttccgg catttcaagt ccgcgaacat cggcaaatac
     3421 caggttatcg gcaaagcggg cattgatctc atcccaatgt gaccggattg actcgttggc
     3481 tagtgttacc ggcccctgcg cagcagggtc tttataaaaa aaggtatgca ccagctgatc
     3541 attgaccatt tcggtataag cgctgaatcc ttgccgcttc aatacagtga tcagcggtgc
     3601 cggtgtaaat gtattgatga tctccagggc ctgcccttct ttcagctcat tgattttctc
     3661 catgatcagt tgaaagggat ctgttccgcc tgccagtacg ggtcggacat ccagttgcac
     3721 cagttgttct ggccgcaaag cgccaatcaa ttcaggttta tctgacatag tattattttt
     3781 tcaataaatc agttcagtct tttttccagt tcaatggctt tagggaaaag gatattattc
     3841 tccaggtgga catgcaggtg aagatcatct tcaaaggcat ccagcatcct gtaaaggaga
     3901 ctataactgg cacaggcatc atcaggcagc gtgtaatgtc ctgtcagttc ccggatctta
     3961 gccaggtgtt cacccaccac ttcatgctcc atttccatca tctgaatggg ctgttgcacc
     4021 gtgcccagtt ctttggcatg ggcagactca gttttatccg caacctttac cagctcatgg
     4081 atataaggga ataatacttt ttcttccttt accatatgcg ccatcatctc cgtgttcacc
     4141 tcggataccc gttgccagat ctccagtagt tccggatgcc ggccgccatg aactttacgc
     4201 actttatctg cataagctac aatatcgggc agattgttac gtacatagct gtggtgtgta
     4261 tttacaataa aatcacagag aaaagccgga ctccagtcat tatacggcag cggattccgg
     4321 atggcttctt ctttttcgga taattgtaac tcacgttcca ctttggttac atccaggcct
     4381 ttttcttggc aggcttcttt gaccgtcttt tttccaccgc aacaaaagtc cagcccatat
     4441 tttttaaaga tctgtgcttt gcgcatgtct tttgccacca gttgtcccag ggtttcctcc
     4501 gcttcgccac ttttccgttt cgaaatcctg actttccacc attcaggtcc ttcttccagg
     4561 tactcccagg taaaaatatt gccacgttcc cccaacaact ggtaataaag gggcttgggg
     4621 tcatgatcgt tgtggatggt caggctctct ccctccgtca gttcatcaaa ataccggaaa
     4681 atagtaggat gctttaaccg gggttcaagc agggtgacat taaggatatt gtctgtgagt
     4741 gtttgcataa taaaagtttt gtcaaagata aaaaaggaaa tttaataaaa gtatttaaat
     4801 acttttattt actgaattcg aaatcttttc ccctgattat gacaaaaaca ggacatttcg
     4861 gaccaggatt gcgataaaaa ccctgaatat taaactgcag cgtgccggtt ccagttaatt
     4921 ttattcccga tccccaatgt attatcggta aattttacat gatcaaggcg aatagtccag
     4981 atcgctccgg gcatggccag tgcaaccgga tgctttttat gataatgggc agctgcatgt
     5041 gccgccaacg gatcagcagc aggaagaatt accccttcga attgcactcc tttaatggcc
     5101 aaaaaattga gtttatcggg gaggatcgtc ccggaaactt tgggattagc atgcagaata
     5161 accgaatgac gggtgtcatt ggaggatttg taatacagca aatcattcgc tacatcaacc
     5221 gaatagaaaa aactgaaaca ccaggaattt cccagttcat ccacacaagc caggttacca
     5281 caagtttgct ttgaaataaa ttccgcaatt ttttcgttca tgatattttt attacccgca
     5341 ccggacaggc caatgcgatt tcccgggact tatcaagtgc tgaagcaggt atctccagca
     5401 catagacctc tttctttttt attcctttta gtaacgtggc cttaccatcc ttttttgaca
     5461 tacgccacat ttcagattgc tgttcatagc aaaccccgca gccaatgcat ttatcccggt
     5521 agtggattac cttaggcatg ggcggcttcc acaattttat acagcttatc cgaaggggtt
     5581 actttagtgt caaacttcaa cgtaatctta tcgcctttta ccgcttccgt ggccggttcg
     5641 ccattcacca tcagggtgga gatcgtttct ttgatcatcc cgcatttcgg accggtgacc
     5701 atcatcgtat ctcctatcct gagtgagccg gattcgagga taaactcacc aattgatatt
     5761 ttcggataat atttactccc cttgccgatg tatatcttct tttcagtggc ggccgaaccg
     5821 ggctgtgccg tccattcacc cagtttacgt cccagataat aaccctccca gaaaccgcga
     5881 ttatacacgg tttccatttc ctttttcaag gcggccgctt tttcaagtgt atatgttccg
     5941 tccagtacgg catctaaagc agcccggtaa catttcgtaa ccacttgcac ataatcggcg
     6001 cccttggttc ttccttcgat cttcaataca tcgactccac tgtcgatcag ttggtccagt
     6061 atatcaatgg tacaaagatc cttgggcgac attatatatt cgttatcgat ctccagttgt
     6121 tcgccggtct cggtatctgt taccgtataa ggccgtcggc aattttgcac acaggcgccg
     6181 cggttggctg ccgcattctg cgtatgcaga ctcatataac attttccgga aatagccatg
     6241 cataaggccc catgcacaaa tagctccagt ttcaccagtt tccctgaagg acctttgatt
     6301 ttcttacgtt caatttcgct gctgatatcc gcaacctgtt ttaaggtcag ttcccgggcc
     6361 agcaccatca catcggcata acgggaaaaa aaccggactg actccacatt gctgatattg
     6421 gcctgggtgg aaatatgcaa cgggatctcc atctggttgc aataatcgat taccgcaaaa
     6481 tctgcggcga tcaccgcatc aattttgtgt ttcttaactt cctttaatac ggttttgagt
     6541 aattgcatgt catactcata catcacggta ttgagtgtga tataggcttt tagtccctgc
     6601 ttcctgcaaa tagcactgac cgttttaata tcagaaatat gaaaggactg ggaagagcgg
     6661 gtacgcatat tcagttgctc cacgccaaag taaatggcat cggcaccggc attgatggcg
     6721 gcctgtaaac tatcgaaaga gccggccggc gccagcagtt ccactttatc ttttctcatt
     6781 ccactgattt atttcatccc ttcgagaatc cggatgtagt tggctctttc aaaagcagta
     6841 gggtctgata cattctgctg actcatgctg cccctgaatg aagtgatatc ttcaaagccc
     6901 atcatgtaca tccagtcctg cagttcctta ttcattgttt tcaggtaagg aattccattc
     6961 ttatacaggg aagaagctgt catcaccaca tcggctccag ccagcaggta tttgaccact
     7021 tccactgcac tttgcacccc ggtcgtagcg gccagtgata atttcacttt ttcaaataat
     7081 aaggcaatcc ataacaacgg caaacggatc tcgctgggtt cactgtaatg cagatctgtt
     7141 ttgagcacca gctcattgat atcaaaatcc ggttgataaa accggttaaa caataccagt
     7201 gcatcggcac cggaattttt catacgcatg gccatattcc cggtcgcgct gaaataagga
     7261 ttcagtttca cggccaccgg tatcttcaca ttggacttta tcgcttctat aatattcagg
     7321 taacgatgct ccacttctga agaagacatg gtgatatccg ccgggataaa gaatatattc
     7381 acttccagtc catccgcacc tgcctgttcg atctgtttgg agtaatcaat ccaaccctcg
     7441 gttgtaatcc cgttcaggct gccaataatg ggaatattca cgcgttcctt cgctttccgg
     7501 atattctcca ggtattcatc cacccccaca ttaaaatcat cgaggtcagg aaaataatcc
     7561 aatgcctccg ggaatgcata cgaggtttca gacataacgg tattcatcct tgcttcttcc
     7621 ttccttatct gctcttcgaa cagggaaaac atcaccacgg cacccgcacc attatcttcc
     7681 atttttacga tattatcaag ctgttcggac aaagtgcagg cagaaacaac aatgggtgaa
     7741 ttcagtttca gccccatgta agtggattgg agtgtcatat acttgttttt tatacaattg
     7801 tcctgaagct tatggccact tcaggacaat ttagatttac ttaattcttc ccgggcatgt
     7861 aacatgcgac aggcttatcc tatagggaca aaatcactgg ctttcttcgt tgccagttct
     7921 tcataggttt tccatttcag gttgaccagg tcctgagcat gattcatcag gtgctcagct
     7981 acttcaggat ttgtcatcgt gagtacttta tacctcagct cattataggt atagtctttc
     8041 aacgtaatcg ttggcctggt ggaatccagc acaaacggat tctgattttt cttccgcagc
     8101 atcgggttgt aacgcaacag cggccagtga ccactggcaa ccgctttttg ttgctgatcc
     8161 agtccgtttt tcagatcata cccgtgagca atacaatggc tgtaagcaag gattaatgat
     8221 ggcccatcat acgcttctgc ttcccgcatg gccagcaggg cctgttgcgg attggcgcca
     8281 aaagccaccc ttgccacata tacattaccg taagagatcg cctgcatcgc caggtctttt
     8341 ttaccacctg ctttaccacc ggctgcaaat tttgcggtag ccgctgtcgg tgttgacttg
     8401 gaagactgac ctcccgtatt ggaatatact tcggtatcaa ggacgagtac gttaatgttc
     8461 cgtccgcttg ccagcgcatg gtccagacct ccatagccga tatcataagc ccatccgtcc
     8521 ccaccaatca gccatacact gcgacgtacc agttggtccg ccactgaaag cagatgcttc
     8581 gctgcaggtt tgtcaatgga cctcagtttt tccattaaat catccacccg gactctttgt
     8641 tccaccagtt ctgactcgag ggtttgtttc gcttccacaa tatcttttac atatccttca
     8701 cccagttcac ccttcagttc atccaggtat tgatgcgcca cactgagttg cttatcagcc
     8761 gtgatccgca tacccaatcc aaactcggca ttgtcttcaa acaacgagtt actccaggct
     8821 ggtccgcgtc cttctttatt taccgcccac ggggtggtag gcaggttacc gccatagata
     8881 gaagagcaac ccgttgcatt cgcaacaagt aaacggtctc cgaataattg ggacaacagt
     8941 ttcaggtaag gtgtctcccc acaaccggca caggcaccgg agaattcgaa caggggttcc
     9001 agaaactggg caccatgaac ggtggagaaa tcaatttttg accggtcatt ccagggcacg
     9061 ttctcaaaga actcaatact tttctgctcc tgtttaagaa tcggatcttt atccaccagg
     9121 ttgatagctt tacgggcacg gttggttggc tcagtagccg gacaaacctc cacacataaa
     9181 ttacatcctg tacaatcttc tatataaacc tggagggtgt atttggtttc cgggaaacct
     9241 ctcgcattga tcggggcgga tttaaatcct tccggtgctt tttctaaatg gttctcatga
     9301 taaaacttgg cacggataca actatgcgga catacataag cacagttacc acactggata
     9361 caaagatccg gttcccacaa cggcaccagg tcggacacat ttctcttttc ccattgtgtg
     9421 gtcccgctcg gataggttcc gtcaatggtc attttactca ccggaatatc atccccgtgg
     9481 cccgacatca tcatagcggt cacatcttgt acaaaagcag gtgctttatc cgaaacggtc
     9541 agcagttcaa ccatggcaga ggatgcttta gccggaatct ccacctgata caggtgcgcc
     9601 agtgtggcat ccacggcctt gaagttctgc tcgattactt tcggaccttt tttgaaatat
     9661 gttttctcaa tcgctttctt gatctgctta atcgcatcgt ctttcggcag tacaccagac
     9721 aaggcgaaat aacaggtctg catgatcgta ttgatacggc cactcatacc cgtttcgcgg
     9781 gcaacggtag tggcatcaat cacatatacg gtgatttttt tatcgataat ctgctgttgt
     9841 acctgaccgg gcaatttgct ccatacttca tccttatcaa aaggactgtt caacaggaag
     9901 gtagctccgg tgctggcaaa ctccagcatt tccactttct ccacaaaatt gaactggtga
     9961 caagctataa agtcggcttt ggaaatcagg taaggcgcgc ggatcggttc aggaccgaag
    10021 cgcaggtgag aaacggtacg ggctccggac tttttggagt catacacaaa atatccctgg
    10081 gcaaacagat ccgtattctc cccgataatc ttaatggtgt tcttattggc accaacggta
    10141 ccatccgcac caaggccaaa gaaaagtccc tgtctccagg ctgattcatc cagtttatat
    10201 tccggatcaa attcaagact ggtaaaggtt acgtcatcat taattccaac cgtgaaacca
    10261 ttcttcggat tatctttttt caattcatca tatactgctt tcaccatggc cggggtaaat
    10321 tctttggaag aaagtccgta acggccgcca acgattctcg gtaacgtttt aatttttccc
    10381 agctggaagg cttcgaccat agaggccagt acatcctggt acatcggttc accagctgcc
    10441 ccggattcct tggtacgatc cagtacagca atgatctttg tgctggccgg gatggctttt
    10501 acaaaatgat ccatggagaa cggacggaag agacgtacat gcagtacccc gactttctca
    10561 ccggctttct gcagcgcttt gacagtttca atgacggtct caccaccgga acccatcaca
    10621 atcaccacgc gttctgcatc tgcggctccg ctgtattcaa ataactggta tttacgtccg
    10681 gtcattttgg cgaagtcatc catcacgtcc tgtacaatac ccggtgtatt attataaaag
    10741 gtattcacgg tttcgcgacc ctggaaatac acatcgggat tctgggccgt accacgtata
    10801 aaaggatgat ccggattcaa cccccgtcca cgatgggcaa tcaccaggtc atcgggcacc
    10861 atggaccgga tctgatcatc actcagcagt tccagtttat ttacttcgtg tgaagtacgg
    10921 aacccgtcaa aaaagtgcac aaagggaatt cttgatttca gggaagccac ctgggagata
    10981 agcgcaaagt catgggcttc ctgaacactg gcggatccca gcatggcaaa gccggtggta
    11041 cgggcggcca tcacatcctg gtgatcacca aagatggata gaccctgtgc ggccaatgaa
    11101 cgggcggcca cgtgaaatac cgtagcggtt aattcaccgg caatcttata catattgggc
    11161 agcatcagca tcaaaccctg ggaagcggta aacgtagtgg tcagtgaacc ggtctgcagg
    11221 gcgccgtgta ccgttcctgc ggcaccacct tcactctgca tttcaattac atcgggaacg
    11281 ttaccccaga tgttttttgt tcctttagct gcccattcat cggccagttc cgccatattg
    11341 gaagaaggcg taatgggata aatcgcacaa acctcgttga tgcggtaggc cacataggcc
    11401 gcggcttcat tgccatcaat ggtggcgact gatttatttt tatttgacat ctgtagtaga
    11461 attaacaggt tcagaaatca tatctattgc atgacaggga cattgctcat aacaaaccgc
    11521 acatccggtg caggcattgt aattgaactt ataccggttc cccttgccta atttgatgat
    11581 ggcatcttca ggacaagcac caaaacaacc atcacattca aaacagtttc cacaactcag
    11641 gcagcgttgt gcttcaaagc gggcttcttt ttctgagagg ccactgatca cttcgtcaaa
    11701 acttttcacg gccacagcag gagccagttt atcctgttct ttctgcgggg catccgtttt
    11761 ataccacata tgcagtttcc ggaacccggc agtcggatgc ttatcaggtt tctggtaagg
    11821 atcctgcatc aggaaaccat gcatatattt agcggctttc ttaccatgtc cgatggcaat
    11881 ggtggtgctt ctgttttcac caggtagcat atcgccgcct gcgaaaattc cttcatgacc
    11941 ggtcatccgg agtccatcga tccccacagt accatcatcg tttactttta taccagtagc
    12001 ggaacggagg aatccggtat cggattcctg cttattggcg ttgatcagca catcggcatc
    12061 gacggtttca aactcaccgg ttcctaccac tttccctttc tccacttttg ttttttccag
    12121 ggtgatttta tttctctcaa ttttactgat gctgcggaac aactgcacat ccacaccttc
    12181 tgccagggca tcttcggttt cgtaatcata agccggcatc atttttttat ccccgggaaa
    12241 ataaacggat acttcagaac cgaagcgctt gatcatacgg gagagatata aagccagttt
    12301 accaccgccg tacaccacca cttttttctt aacatatggg gaagaattca atttggcttc
    12361 gcggaaaaat tcgaaggcat cggtcatgtg tacagaacca tcctgcggaa atgtttccgg
    12421 acggatcaat tgggcgccga tggaaagata aacggcatcg aaattaccag cttgcttctc
    12481 tgtaagcaca tcctgtactt tatagttcag ccggatcttg acgcccattt taacaatgcg
    12541 gtccacttcg gcatccagta tttcttttgg caggcggtaa tccggtatac cggtccacat
    12601 cagaccgccg ggatgtttac ctgcgtcata tacttcaaca gtatgtccca gtctggcgag
    12661 gtgataagca gctgaaagtc cgcccggacc agctcccacc accagtactc ttttcccggt
    12721 tggtactgcc tcaaaacgaa ccggccattt ctggctgatg gcttcatccc cgatataccg
    12781 ctccaccgcg tgaatattca ctgtggtttc gatatgattc cggttacaac cggtttcaca
    12841 aggcttaaca caaacccttc ccattatggc tgggaaagga caatcttcca gtaaggtctg
    12901 gaaggcttca aaataattgc cggattgtgc atgcgacaac cagccctgaa tgttctgacc
    12961 agcaggacag gcactgttac aaggaggcat aaagtcaaca tagacaggac gacgggaacg
    13021 tagcggaccg gttccttccg catgggtgag gaggtcaacc ggttttgtaa tatcatttgc
    13081 agccatgatg aatggtttaa agggcaggcg gtatgcatac ccatgaatag gctttttaaa
    13141 aaccaaagca aaatagaacc attcggcaag gtggggggtg acgtgattca gtcaaaaaaa
    13201 tgattcctat catgttattc gacaatcgct taccggggaa tgggg
//
LOCUS       DAOPMW010000054        13021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4906_length_13021_cov_8.231066, whole genome shotgun sequence.
ACCESSION   DAOPMW010000054 DAOPMW010000000
VERSION     DAOPMW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13021
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_13021_cov_8.231066"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..2246)
                     /gene="nagB"
                     /locus_tag="PLU11_04655"
     CDS             complement(318..2246)
                     /gene="nagB"
                     /locus_tag="PLU11_04655"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPN58437.1"
                     /translation="MSKKAAPRNFADSFEKIPVHVFPNQKDGSVYVARLIADYIREKQ
                     KKKEKCVLGLATGSTPKTLYAELVKIHREEKLSFKNVVTFNLDEYYPIANNALQSYNR
                     FMKTHLFDLVDINPKNIHIPNGEIKKEDIKEHCRHYEKMIEDAGGIDLQILGIGNNGH
                     IGFNEPGSGIYSRTRLITLDNSTRIANSYEFANISEVPRLAITMGISTILKSKKIILM
                     AWGSAKAPAIQKAVEDDDSEQMPASLLQNHDDVSFVVDDAAAAELTRIKSPWLTGDCE
                     WTPMMIKKAVVNMALKLKKPVLSLTNSDYNEYGLSDLLVDQGDAYEINLQVYYMLRDS
                     ITGWPGGKPNVNLPVHPERSSPYPKRVIIFSPHPDDDIISMGGTFQRLHDQGHDVHIG
                     YQTSGNIAVTDEFVTRFLDFAVGFEEIAGIDTKKSGKILEDARKFINRKKSNQMDTPT
                     IRAIKGLIRRGEAKATCRYVGIKDENIHFQNLPFYETGAIEKKPMSEKDVKITIELLR
                     QIKPHQVYCAGDFADPHGTHIVCFNVVLEALKRIKAAGDEWIGDCWLWLYKGAWQEWN
                     IEEIEMAIPMSPDQVLKKRFGIFIHQSQKDMVPFQGSDSREFWQRAEERNSATANLYA
                     DLGLTHYAAMEAFVRWHY"
     assembly_gap    2263..2362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2363..4524)
                     /locus_tag="PLU11_04660"
     CDS             complement(<2363..4524)
                     /locus_tag="PLU11_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPN58438.1"
                     /translation="MRKIAIGLFLGFFTLSASTQTVHIIPQPASVKQPKINARFSISP
                     ATVLVLEGSNLDKTAKYINEYLEQFYQFKLNILSNTISKNAIRLNFERMDNALPGAYR
                     LSVNGNGVYIAGDNEEGVFYGIQTLIQLLPVPDARLKLAGTRLDIPHVSVEDAPRFEY
                     RGMHLDVGRHFLPVSFVKKYIDYLALHKLNTFHWHLTEDQGWRIEIKKYPELTKMGAW
                     RNGTIIGRYPGKGNDNIPHGGFYTQQEIKEVVQYARERYVEVIPEIEMPGHSSAAIAA
                     YPWLSCFPEKPTAIPANMISGKTEDGLERGGIKYVQETWGVFDDVFCAGKESTFEFLQ
                     NVIDEVNTLFPSSYFHLGADECPKTHWKKCEACQKRMKENNLKDEHELQSYFVQRIEK
                     YLNSKGKKLIGWDEILEGGLAPNAVVMSWRGEAGGIEAAKQKHGVIMTPGNPVYFDHS
                     QSENEDSITIGGYNPIEKVYAWDPVPAELNAEEGKYILGAQANVWTEYMDNPRKVEYM
                     IFPRMAALSEVLWSKKENKNWTAFEKKLVPAFKRYDLWKANYSKAFYDIKSKVIPASS
                     GNGVLWELTGRLAEKIIVERKGPYPSFVYTKPHPVTSSTTFSARNTKLILWQTFIFNK
                     ATGKKITLTTPPSKSYPGDGAFTLVNGVQNVKGLAKSKEFLGFSGADCEAVIDLGKEE
                     MVSKAVVHTLRQTGSWIWQPLVTEVFVSSDGSNYESAGL"
     gene            4575..5045
                     /locus_tag="PLU11_04665"
     CDS             4575..5045
                     /locus_tag="PLU11_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN58439.1"
                     /translation="MQTNAEVINRFYTAFQQKDYQGMNACYSDDIVFSDPAFGLLRGE
                     EAKYMWEMLCKNAKDFSLTFSDIELLDEEYATCKWTASYTFSQTGRKVVNRIKAFMRL
                     KDGKIIEHSDAFPLSKWAAQALGWKGELFGWMGWMKRAIQKKARKNLVAFIESK"
     gene            complement(5126..6001)
                     /locus_tag="PLU11_04670"
     CDS             complement(5126..6001)
                     /locus_tag="PLU11_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPN58440.1"
                     /translation="MNTKNIEHILPPPAPHMVGDGFRVHSFFPGGRLIDKKRMSPFFL
                     MDYGAKIEFNPSEEPRGVGVHPHAGFETVTIAYHGRIAHHDSAGNSGIIGEGDVQWMT
                     AGSGLLHKEYHEKEYSKTGGPFQMVQLWVNLPAKDKKTTPKYQELTNQKMGRHSLTDG
                     KSYIEVIAGTYQGTKGPAFTFTPMEVYNARLQASGRATLTLPAHYNTGLLIIQGSAVI
                     NGQDVAADHFVLFKNDGEEITLEAKEDSIILVLSGEPIDEPIAAYGPFLMNTWPEVEQ
                     AIQDVNAGKFGYLED"
     gene            complement(6180..6896)
                     /locus_tag="PLU11_04675"
     CDS             complement(6180..6896)
                     /locus_tag="PLU11_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPN58441.1"
                     /translation="MKTILHKAATRGQANHGWLNSYHTFSFAGYQDADRIHFGALRVL
                     NDDTVAGGMGFGAHPHDNMEIISIPTSGDLEHQDNMGNKQVIRQGDVQVMSAGTGIQH
                     SEKNKNKDKTVEFFQIWVFPNQRNVTPRYDQQSFTDADKHNKLLTVVSPMGTTDGKVH
                     IHQDAWFSLGKLDKDFLTEYKLFKKDNGVYAFVIEGNVTINGISLNRRDGLGITEAEV
                     LSIKADSDAEVLLMEVPMLK"
     gene            complement(6907..7473)
                     /locus_tag="PLU11_04680"
     CDS             complement(6907..7473)
                     /locus_tag="PLU11_04680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPN58442.1"
                     /translation="MKIVILSGSPRINSVTVRVALHLNNWFKKNTTHEIDLIELKDWN
                     LPPVQSVWTAPEKAPTEFQSLAQRIFAAEAFILVSPEYNGGYSPALKNLLDHFPKQLH
                     KPFGIVTASPGAMGGMRASQQLLQLVPALFGIASPNMLITPAVDKKFSPDGDLLDESF
                     QGMVHNFVTEFLWLAEKLVIQKVVTENK"
     gene            7560..8642
                     /locus_tag="PLU11_04685"
     CDS             7560..8642
                     /locus_tag="PLU11_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HPN58443.1"
                     /translation="MGIVLETINFEGIKVKTMVHRRKFIQSSALGSLGVLFSKIAAGA
                     GTPSSVKGRPVVISTWDAGLEANKGAWEVLGKGGRALDAVEKGVMVTEASQNCCVGLG
                     ANPDRDGFVTLDACIMDEFSNCGSVAFLERIKHPVAVARRVMEKTPHVMLVGSGAQQF
                     AVAEGFPLEEQKLSPDAQRTYEEWLKKSDYKSPIINVENKGHGPFAPYKLDNGEWNHD
                     TIGMVAMDTFGNLSGSCTTSGAGFKMRGRLGDSPIIGAGLFVDNEVGACTATGQGEDV
                     IRVAGSHSVVELMRQGLSPEAACKKIIERIARIKKEKAKDIQVAFLALNKQGVAGAYA
                     LHPGFSYAIKSGTKETLIKAKSYFGS"
     gene            9014..9751
                     /locus_tag="PLU11_04690"
     CDS             9014..9751
                     /locus_tag="PLU11_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPN58444.1"
                     /translation="MNDKKYIIEIATSDFLTTRSAVEGGADRIELCANLAEGGTTPTY
                     GTVKQCREAFSVLLYPIIRPRGGDFLYTDEEFSIMLQDIKLFKELGCDGVVIGLLNRD
                     GSIDVPRSTQLVEAAYPLGVTFHRAFDRCRDPFEALEQLVEMGCERILTSGQLPTVSE
                     GADLIAELNRKAEERIIIMPGSGLRKENIKWLAEKTGCQEFHSSLRGKAASFMEFIHP
                     GFAGSAESYTNNAIDPEEVRALRAALD"
     gene            complement(9799..10674)
                     /locus_tag="PLU11_04695"
     CDS             complement(9799..10674)
                     /locus_tag="PLU11_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPN58445.1"
                     /translation="MQYNRRNFLKFTGAVGTGLVLNSLGSSSLFAAETAKPISQFGIQ
                     LYTLRDVIPGDPKGVLKQLASFGYKQIESYEGAKGMFWGMGHTGFKNYMDELGMKIVS
                     SHCDITKDFEKKAAEAAEIGMKYLICPWKGPQKSLDDFKRFADEFNQKGEICKKNGIR
                     FAYHNHDYSFKAMDGQLPQDVMMNGTDPSLVDFEMDIYWVAAAGVDIEAWFKNYKKRF
                     RLCHVKDRSKNPGTDNGKNSVDLGTGSIDWPTILKSAKKYGMQYYIVEQEAYPNGSSL
                     EAAKVNADYMKTVKI"
     gene            complement(10693..11304)
                     /locus_tag="PLU11_04700"
     CDS             complement(10693..11304)
                     /locus_tag="PLU11_04700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HPN58446.1"
                     /translation="MDRRELLKKIAILTGGVVIGGEVFLSGCTTGAKASAFSPETIAL
                     LDEIGETIIPATATPGAKAAAVGEFMKVMITDCYTEAEQKVFTDGIEKLQEVSKKMHD
                     KSFMECTPQQRHDLLLSLEKEAKQFNDKKNEAEKEQREIAKATKQPFVGAPPHYYTMM
                     KQLTLWGFFTSKTGMTETLRHLPVPGRYDGAFPYKKGDKAWAE"
     gene            complement(11338..11793)
                     /locus_tag="PLU11_04705"
     CDS             complement(11338..11793)
                     /locus_tag="PLU11_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPN58447.1"
                     /translation="MGTVFKVEMSSGNPYKKGGMFEGASYLIFEKAKQLRKNMTPAET
                     VLWMHLKAGLEGYKFRRQHPIGIYIADFYCHRAKLIIELDGSIHQLEEVQQNDNKREA
                     DLLEWGYQVIRFSNQEVFNKINFVLNRILADIKLIINNKNLNASPDSGV"
     gene            complement(11828..>13021)
                     /locus_tag="PLU11_04710"
     CDS             complement(11828..>13021)
                     /locus_tag="PLU11_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPN58448.1"
                     /translation="SLEGLEVLPDGDFMPAMELNCVEKDVAAKIKNHFKGSRHMIIGR
                     SANLTQPHHDRTNCQYRNRCWRGCPFGGYFSTQSATLPAAMKTGNLTLRPFSIVSKVL
                     YNKDTKKATGVEIVDTMDKKTYEFKAKIVFLCASAFNSTWVLMNSASDIWPEGLGSSS
                     GELGHNVMDHHFRCGAGGKVEGYSDKYVYGRRPTGVYVPRFRNIYEDKRDYVRGFGYQ
                     GSASRGRGMEVAEFTIGADLKNALTEPTDNWNIGIMGFGEMLPYHDNKITLNKDVKDA
                     WGLPVLNMDVEIKENEHKMRIDMMNDAKEMLEAAGVKDVNSYDHGYEVGMGIHEMGTA
                     RMGRDPKSSVLNGNNQVWDAKNVFVTDGACMTSASCVNPSLTYMAMTARAADFAVSEL
                     KKGNL"
BASE COUNT         3227 a         3033 c         2942 g         3719 t
ORIGIN      
        1 gcttgccggt atatcggatg atcatcggtg atcatcaccc ggatgggctt tttcatttta
       61 ctaatggtaa tttaatccgg gtaacgggcc aattcgatca gcgatagagt tgagggtttg
      121 acgagatcaa ccggaacaca aatttccggc ttcagtattt taaaaaaaat accccttttg
      181 tggtatgggt acttagtcgc atctacgtaa atacacttag ttcaagtatt gaaaacaaat
      241 ggtgacctcg cagaaagtta aaagaccggc cacaaaatac aggccggtct ttttaaaggt
      301 caattttatg aatatagtta ataatgccag cggacaaagg cttccatggc agcgtagtgg
      361 gtgagtccta agtctgcata gagattggcc gttgctgaat tccgttcttc cgcacgttgc
      421 cagaactcgc gcgaatcact tccctggaag ggaaccatgt ctttctgtga ctgatgaata
      481 aaaatgccga aacgtttttt gagcacctgg tccggactca tggggatcgc catttcaatt
      541 tcttctatat tccattcctg ccaggcgcct ttgtataacc agagccagca atctccaatc
      601 cattcatcac cggccgcctt gatgcgcttc agggcttcga gtaccacgtt aaaacaaaca
      661 atatgggtac cgtgcggatc ggcaaagtcg ccggcacaat aaacctggtg gggttttatc
      721 tgacgtaata attcaatggt aatcttcaca tccttttcgc tcatgggttt cttctcgatc
      781 gccccggtct catagaaggg caggttctga aaatgaatat tttcatcttt tatccctaca
      841 taccgacaag tggctttggc ttctccacgg cggatcagtc ccttaatggc ccttatcgtg
      901 ggtgtatcca tctggtttga ctttttccgg ttgatgaatt tccgggcatc ttccagtatc
      961 ttacccgatt tttttgtatc aataccggca atctcttcaa agccgacggc aaaatccaga
     1021 aagcgtgtaa caaattcatc ggtcactgca atattgccgc tggtctgata accgatatgt
     1081 acgtcatgtc cctggtcatg caaccgctga aaagtaccgc ccatactgat aatatcatca
     1141 tccgggtgag gggagaagat aatcacccgt ttgggataag gggaagagcg ttcggggtgt
     1201 accggcaggt ttacattcgg tttaccacca ggccatcctg ttatagaatc ccgcaacata
     1261 taatacacct gcagattgat ttcataagca tcaccctgat ccacgaggag gtcgcttaag
     1321 ccgtattcat tataatcact gttggtcaga ctcagcaccg gcttcttcaa tttgagggcc
     1381 atattcacca cggctttctt gatcatcatc ggtgtccact cacagtcacc ggtcagccag
     1441 ggtgatttaa tccgggtaag ttcagcggcg gctgcatcat ccaccacaaa ggatacgtca
     1501 tcatgatttt gtaataaaga cgccggcatc tgttcgctgt catcatcttc caccgccttc
     1561 tggatagcag gtgctttggc actgccccag gccatcagga tgatcttttt cgatttcagg
     1621 atggtgctga tgcccatcgt aatagcaaga cgcggcactt cggagatatt ggcaaactca
     1681 taggagttgg cgatccgcgt ggagttatca agggtaatca gccgcgttct tgaataaata
     1741 ccggaaccgg gttcgttgaa accgatatgc ccgttattac caatccccag tatctgcaga
     1801 tcgataccac cggcatcctc aatcatcttt tcgtaatgac ggcaatgttc cttgatatcc
     1861 tctttcttga tttccccatt ggggatatga atatttttcg ggttgatgtc caccaggtca
     1921 aacaagtggg tcttcataaa ccggttataa ctctgcaggg cattattggc aatcgggtaa
     1981 tattcgtcga gattaaaggt caccacgttt ttaaaactca gcttctcttc gcggtgaatt
     2041 tttaccagtt ccgcatacaa tgtctttggc gttgaaccgg tggccagtcc caatacacat
     2101 ttttcttttt tcttttgttt ctcccggata taatccgcga tcaaacgggc cacataaaca
     2161 gagccgtctt tttgattagg aaatacatga accgggattt tttcaaaact atcagcaaaa
     2221 ttgcggggag ctgctttttt tgacatgtag aggtggtttg agnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnagtccggc tgattcataa ttgctgccgt cggatgatac
     2401 aaatacttcg gttaccaatg gctgccagat ccagctgccg gtctgacgaa gggtatgtac
     2461 cactgcctta ctgaccatct cctccttacc cagatcaatc accgcttcac aatccgctcc
     2521 actgaaaccc aggaactctt tgctttttgc cagtcctttt acattctgca ctccatttac
     2581 caatgtaaat gcgccgtcgc caggatagga tttggatgga ggggtggtta aggtgatttt
     2641 tttcccggtg gctttattga agataaaagt ttgccagagg atcagtttgg tatttcttgc
     2701 cgaaaaagtg gtgctgctgg ttaccgggtg tggctttgtg tatacaaatg aagggtaggg
     2761 ccctttacgt tctacgatta ttttttcagc taaccggccg gttaattccc acagtactcc
     2821 attgccggaa gaagcgggta tcactttgga ttttatatca taaaaagcct tactgtaatt
     2881 ggctttccac agatcatatc ttttgaaagc cggcaccagt ttcttctcaa aggccgtcca
     2941 gttcttgttt tcctttttac tccacaacac ttcactcagg gccgccatac gcgggaagat
     3001 catgtactcg actttacggg gattgtccat gtactccgtc catacattgg cctgcgcccc
     3061 aagtatatat tttccttctt ccgcatttaa ttccgcagga accggatccc aggcatatac
     3121 tttttcaatc ggattatagc cgccaatggt aatactgtct tcattttcac tttgtgaatg
     3181 atcaaaatag accgggttgc cgggtgtcat aatcacgccg tgtttttgtt tggccgcttc
     3241 gattccgccg gcttctcctc tccagctcat caccaccgca ttcggggcca gtccgccttc
     3301 gaggatctcg tcccagccga tcagtttttt gcctttgctg ttcaggtatt tctcaatccg
     3361 ctggacaaaa taactctgca gttcgtgttc atctttcagg ttattttctt tcatcctttt
     3421 ctggcaggcc tcacattttt tccaatgggt cttgggacat tcatcggcgc cgagatgaaa
     3481 atagctggag gggaataaag tgttaacctc atcgattaca ttttgcagaa attcaaatgt
     3541 ggattccttg ccggcgcaga atacatcatc aaatacaccc caggtttcct gcacatattt
     3601 gattcctcct ctttccagtc cgtcttcagt tttacccgag atcatgtttg ccggaattgc
     3661 ggtaggcttc tccggaaaac aactcagcca gggataggcg gcaatggccg cactgctgtg
     3721 accgggcatt tcaatctccg ggattacttc tacataacgc tcacgggcat actgtaccac
     3781 ctctttaatc tcctgctggg tataaaagcc tccatgcgga atattatcat tgcctttgcc
     3841 cgggtagcga ccgatgattg taccattgcg ccaggcaccc atctttgtca gttccgggta
     3901 cttctttatt tcaatccgcc agccctgatc ttcagtcagg tgccagtgaa aggtattgag
     3961 tttatgcagg gcgagataat caatatactt tttaacaaag gaaacgggca gaaaatgacg
     4021 gcctacatcc aagtgcatac cccggtattc aaatcggggt gcatcttcca cacttacatg
     4081 ggggatatcc aaccgggtgc ccgccaattt taaccgggca tccggaaccg gcaggagctg
     4141 gatcagggtc tgaatgccat aaaacacccc ttcttcattg tcaccggcaa tatatacccc
     4201 gttaccattt acggataaac gataggcacc cggtagtgca ttatccatcc gttcaaaatt
     4261 caaacggatc gcattcttac tgatcgtatt ggatagtata ttcaacttaa actgataaaa
     4321 ctgttccagg tattcattga tatattttgc cgttttatcc aggttgcttc cttccagtac
     4381 caataccgtt gccggactga tgctaaaccg ggcatttatt ttgggttgtt tgacggaagc
     4441 gggctgggga ataatatgca ccgtctgggt gctggcggag agggtaaaaa atcccaagaa
     4501 caggccaatc gctatttttc gcatgtaagt tattttgatg ctttaaattt acagaatacc
     4561 caacaactta cgttatgcaa accaatgcgg aagttattaa ccggttttat accgcgtttc
     4621 agcaaaagga ctaccagggc atgaatgcct gctacagtga cgatatcgtt ttcagcgatc
     4681 ccgcatttgg cttattgcgg ggagaagagg caaaatatat gtgggaaatg ctatgtaaaa
     4741 acgcaaagga tttttcactg actttctcag acattgagtt gctggacgaa gaatatgcta
     4801 cctgtaaatg gaccgccagc tatacttttt cccagacggg ccgtaaagtg gtgaaccgga
     4861 tcaaggcctt tatgagactc aaagacggaa aaatcattga acacagtgac gctttccccc
     4921 tcagcaaatg ggcggcacag gccctgggct ggaagggtga actctttggc tggatgggct
     4981 ggatgaaaag ggcgattcag aaaaaagcca ggaagaacct ggtagctttc attgaaagta
     5041 aataaaatta aatgtgatgc tcctgactgc cgactgccaa ctgccgcctg ccgactgatc
     5101 aatattctaa gaaaataata tccaactaat cctctaagta accaaacttc cccgcattca
     5161 catcctgtat cgcttgttcc acttccggcc aggtattcat caggaaggga ccataggcgg
     5221 cgatgggttc gtcaatgggt tcgccggata aaaccagtat gatgctgtct tctttggctt
     5281 caagcgtaat ttcttcccca tcgtttttaa acaatacgaa atggtcagct gctacgtcct
     5341 gtccgttgat aacggcagat ccctgtatta tgagcagacc ggtgttataa tgcgctggca
     5401 aggttaacgt agcccgtccg gaagcctgta aacgggcatt atacacttcc atcggggtga
     5461 aggtgaaagc cggacccttt gtgccctggt aggtaccggc aataacctct atatagcttt
     5521 tcccgtctgt cagggaatgt cgtcccattt tttggttcgt cagttcctga tatttcggcg
     5581 tggttttttt atcctttgcc ggcaggttca cccaaagttg taccatctgg aaagggccgc
     5641 cggttttgct gtattccttt tcgtgatatt ctttatgcaa aagaccggat ccggcggtca
     5701 tccattgtac atcgccttca ccaatgatcc cgctgttgcc ggcactgtca tgatgcgcaa
     5761 tccgtccatg ataagctatc gtcaccgttt caaaaccggc atgcggatgt acaccaacgc
     5821 cacgtggctc ttcggatgga ttaaattcaa tcttcgcccc atagtccatc aggaaaaaag
     5881 ggctcattct tttcttatca atcagccttc cacccggaaa gaaagaatgt acgcggaatc
     5941 catcgccgac catgtgagga gccggaggag ggaggatatg ttcgatattt ttggtgttca
     6001 tgttgaatat tatttatata gaatttttga tttcagttag tagttagtag tcggtagtta
     6061 gtagtcaaca gccgacagcc catacaaagg aggttgtggc ttagcaaaca gctgttactt
     6121 gttttacggt tttcctcacc actccccact cactactcac cactcaccac tcaccaacct
     6181 tacttcagca ttggtacttc catcagcaac acttccgcat ctgaatctgc tttgatggac
     6241 aatacttccg cttccgtgat gccgagccca tctcggcggt taagggagat gccattgatg
     6301 gttacatttc cttcaatgac gaaagcataa acgccattgt cttttttaaa cagcttgtat
     6361 tccgttaaaa aatctttgtc cagtttgccc aggctgaacc aggcatcctg gtggatatgg
     6421 accttgccgt cggtggtgcc catgggagag accactgtga gcagtttatt atgcttgtcc
     6481 gcatcggtaa aactttgctg atcataccgg ggagttacat tccgctgatt ggggaatacc
     6541 cagatctgga aaaattccac ggtcttatct ttattcttat ttttttcgga gtgttggata
     6601 ccggtgcctg cactcattac ctgtacgtcg ccctggcgaa tcacctgttt attgcccata
     6661 ttatcctggt gttcaagatc gccactcgtg ggaatggaga tgatttccat attatcgtgc
     6721 ggatgggcgc caaaacccat acccccggca acggtatcgt cattgagtac acggagcgct
     6781 ccgaagtgga tacggtctgc gtcctgatag ccggcaaaac taaacgtatg ataactgttc
     6841 agccagccat ggtttgcctg tccgcgggtg gctgctttat gtaagatggt tttcatgatt
     6901 ttcgttttat ttattttcag tcaccacttt ttgtataacc agtttttcgg ccagccataa
     6961 aaactccgtg acaaagttat ggaccatgcc ctggaaactt tcgtccagca ggtcgccgtc
     7021 cggactgaat tttttatcca cggccggggt gatcagcata ttgggcgagg caataccgaa
     7081 cagggcaggc accagttgta ataattgctg ggaggccctc attccaccca tcgcaccggg
     7141 tgaggcagtt acaataccaa aaggtttgtg taactgtttg ggaaaatgat ccagcaagtt
     7201 ttttaaggcc ggtgaataac cgccgttata ttccgggctg accagtataa acgcttcggc
     7261 agcaaagatc cgctgagcga gagactgaaa ttccgtcgga gctttttcag gagctgtcca
     7321 aacggattgt accgggggca ggttccagtc ctttaattca atcaggtcaa tttcatgtgt
     7381 ggtatttttt ttaaaccagt tattcaggtg cagcgccaca cgtacggtta cactgtttat
     7441 tctcgggctg ccggagagaa tgactatttt catttttctt agtttataag tcaaaggtac
     7501 ataatcaatg tttaaacatc aaatatttta ttcttagtag tatgttaaac ctgtttttta
     7561 tgggtattgt gctggaaaca attaatttcg aaggcattaa agtaaaaaca atggtccata
     7621 gaagaaagtt cattcagtcc tccgcgctgg gttcattggg ggtattgttc agtaaaatcg
     7681 cggcaggtgc cggcactccc tcatccgtaa aaggccgtcc cgtggtaatc tccacctggg
     7741 atgcgggact ggaagccaat aaaggagcct gggaagtgct gggtaaaggg ggaagagccc
     7801 tggatgccgt tgagaagggg gtgatggtga ccgaggcttc acagaattgt tgtgtgggac
     7861 ttggcgcgaa tccggaccgg gatggctttg ttaccctgga tgcctgtatt atggatgagt
     7921 tttcaaattg cggaagtgtg gcctttctcg aacgcattaa gcaccccgtt gcagtcgccc
     7981 gtcgcgtgat ggagaaaact ccacatgtaa tgctggtggg cagcggtgcg cagcaattcg
     8041 ccgtggcaga aggtttcccg ctggaagaac agaaactctc tcccgatgcg cagcgtactt
     8101 atgaggaatg gctgaaaaaa tctgattata aatctcccat aatcaatgta gagaataaag
     8161 gtcatgggcc ttttgcccct tacaaactgg ataatggcga atggaatcac gataccatcg
     8221 gtatggtggc catggataca ttcggaaacc tgagtggcag ttgtacgacc agcggtgccg
     8281 gctttaaaat gcggggccgt ctcggcgatt cacccatcat cggcgccggt ctgtttgtcg
     8341 ataatgaagt aggggcctgt accgccaccg gccagggcga agacgtaatc cgcgtggcgg
     8401 gttctcactc cgtagtggaa cttatgcgcc agggattaag tccggaagcc gcttgcaaaa
     8461 aaatcatcga acgcattgcc cggataaaaa aagaaaaagc aaaagatatc caggtcgctt
     8521 tcctggccct gaataaacag ggagtagccg gcgcctatgc cctccatccc ggtttcagtt
     8581 atgcaatcaa gagcggaacg aaggaaacat tgataaaagc gaaaagttat tttgggagtt
     8641 gagagagcgt gaaagcagca agtttctgaa agaagggttt aggaggttta gagagttttt
     8701 aaatagaaag agtctgaaag aagggtttag gaggtttaga gagtttttaa atagaaagtt
     8761 tctgaaagaa gggtttagag gtttagttgt ttagaggtag aatctctttg tattccgccc
     8821 cgttaggggt ggcctgttaa tagaaacaag attttacaag gatcccaaag agccctgtag
     8881 gggcgaaata ttaaattgtt tggggtttag agagttttat agatgtaagc ggttgaaagc
     8941 aaagtttagt tgtttagatg acatttttca gaaagggctg ttccctaaca cctaacagcg
     9001 aacaactttt tatatgaacg acaaaaaata catcatcgaa atcgccacct ccgatttttt
     9061 gaccaccaga tccgccgtgg aaggcggtgc cgaccggatt gagctctgtg ccaatttagc
     9121 ggagggtggt acgaccccaa cttatggtac ggtcaaacaa tgccgtgaag cattttcagt
     9181 tttgctttat cccattatcc gtccaagggg tggtgatttc ctgtacaccg acgaagagtt
     9241 ctcaatcatg ctgcaggata tcaaactgtt caaagaactg ggttgtgatg gggtggtgat
     9301 cggactgctg aaccgggatg gaagcattga tgtgccgaga tcaacccaac tagtggaagc
     9361 tgcttatcca ttgggcgtaa cttttcaccg ggctttcgat cgttgccgtg atccatttga
     9421 agcgttggag caattggtgg aaatgggctg tgaacgcatc ttaacttccg gacaattacc
     9481 cacggtaagc gaaggggctg acctgatcgc tgaactgaac cgcaaggcgg aagaacgaat
     9541 tatcatcatg cccggcagtg gtttgcggaa agagaatatc aaatggctgg cggagaaaac
     9601 gggttgtcaa gaatttcatt cttcattaag aggcaaagcc gcttcattca tggaattcat
     9661 ccatccggga tttgccggct ccgcggagag ttatacgaac aatgccattg atccggagga
     9721 agtgagggca ttgcgggcgg cattggatta aaatatggaa gaaaaaataa acccggaaaa
     9781 actccgggtt tatttttatt aaattttcac tgtcttcatg taatccgcat tcactttcgc
     9841 cgcttccagt gaagatccat ttggataagc ttcctgttcc acgatataat attgcatacc
     9901 gtattttttg gcggacttca ggatggttgg ccagtcgata ctaccggtac ccaggtcaac
     9961 ggaattcttg ccgttatcgg ttccgggatt tttactccgg tctttcacat ggcagagacg
    10021 gaaacgtttt ttataatttt taaaccaggc ttcaatatcc acgccggcag ctgccaccca
    10081 ataaatatcc atttcaaaat ccaccaggga tggatcggtg ccgttcatca tcacatcctg
    10141 gggcaactga ccatccattg ctttgaagga atagtcatgg ttatggtaag cgaaacggat
    10201 gccgttcttt ttacagattt cacctttctg attgaattca tcggcaaagc gtttaaaatc
    10261 atccagcgat ttctgcggac ctttccaggg acagatcaaa tatttcatcc cgatttccgc
    10321 ggcctccgcg gccttttttt caaagtcctt ggtgatatca caatgggaag aaacgatttt
    10381 cattcccaat tcatccatgt aatttttaaa tccggtatgt cccatacccc agaacatccc
    10441 tttagcgcct tcataacttt caatttgttt atagccaaag gaggcgagtt gtttcagaac
    10501 acctttcgga tcaccaggaa tcacatcgcg taaagtgtac aactggatgc cgaattggga
    10561 gatgggtttt gcggtttccg ctgcaaacag ggaggagctg cccaggctgt ttaacaccag
    10621 tccggtaccc actgcacctg taaattttaa aaagtttctt ctgttgtatt gcatagcctc
    10681 gttttttaat aattattcag cccaggcttt atctcctttt ttgtagggga aagctccatc
    10741 ataccggcct ggtaccggga gatgtctcag ggtttctgtc attccagttt tggaggtaaa
    10801 gaatccccag agcgtcaact gtttcatcat ggtatagtaa tgaggagggg cgcccacaaa
    10861 gggttgcttt gttgctttgg caatttcccg ctgttctttc tccgcctcgt tcttcttgtc
    10921 attaaactgt ttggcttctt tttcaagact caggagcagg tcatgccgtt gctgtggcgt
    10981 acattccata aaggatttat catgcatttt tttgcttacc tcctggagtt tttcaatgcc
    11041 atcggtgaag accttctgct cggcttccgt ataacagtcg gtgatcatta ctttcataaa
    11101 ctcgccaacg gccgccgctt tggcaccggg tgtagccgta gccggaataa tggtttcccc
    11161 gatttcatcc aggagcgcaa tggtttcagg agaaaaggca ctggcttttg ctcccgtagt
    11221 acatccggat aaaaatactt ctcctccaat caccacacca ccggtgagga tggctatttt
    11281 ctttaaaagt tcgcgtctgt ccataatatt taacccccca accccctaaa gggggcttta
    11341 cactccgcta tcaggcgaag cgtttagatt tttattatta ataattaatt ttatgtctgc
    11401 aagaattcta ttcaatacaa aatttatttt attaaaaact tcttgattgc tgaacctgat
    11461 cacctggtat ccccattcaa gcaagtcagc ttctctttta ttatcatttt gctgaacttc
    11521 ttctaactga tgtattgatc catccaattc aataatcagt tttgccctat gacaatagaa
    11581 atcagcaata taaattccta ttggatgctg tcttctgaac ttataacctt ccaatccggc
    11641 tttcaggtgc atccataaaa cagtttctgc cggtgtcatg ttttttctaa gttgcttcgc
    11701 tttttcaaag attaaatacg aggcaccttc aaacattcct ccctttttat atggatttcc
    11761 tgatgacatt tcaactttaa agactgttcc caagtctccc tttagggaga tttagagggt
    11821 ttttgtatta taaatttcct tttttcaatt cacttacggc gaaatccgca gctctggctg
    11881 tcatggccat ataggtcagg gagggattca cacaggatgc actggtcatg caggcaccgt
    11941 ccgtgacaaa tacattttta gcatcccata cctggttgtt gccattgagt actgatgatt
    12001 ttggatcacg tcccatacgg gcggtcccca tttcatggat gcccataccg acttcataac
    12061 catgatcata agagttaacg tcttttacac cggcggcttc cagcatttcc ttcgcatcat
    12121 tcatcatgtc aatccgcatc ttatgttcat tctctttgat ttccacatcc atattcagta
    12181 ccggaagccc ccaggcatct ttcacgtctt tattcagggt gattttatta tcatgatagg
    12241 ggagcatttc gccgaagccc ataatgccga tattccagtt gtcagttggt tcagtgagcg
    12301 catttttgag atctgcccca atggtgaatt cggccacttc cattccccgt ccacgactgg
    12361 cactgccctg gtagccaaag ccacgtacat aatcccgttt atcttcataa atattccgga
    12421 agcggggaac ataaactcct gtaggtcttc gcccgtatac atatttatcg ctgtatcctt
    12481 ccactttacc accggcgcca caacggaaat ggtggtccat cacattatgt ccgagttctc
    12541 cgctgctgct gcctagtcct tcgggccaga tatccgaggc agagttcatc agcacccagg
    12601 tggaattaaa agcggaggca cagaggaaaa caatcttcgc tttgaactcg tatgtttttt
    12661 tatccatggt gtccacgatc tccacaccgg tggctttttt cgtgtccttg ttataaagta
    12721 ctttggatac aatagagaaa ggacggagtg tgagattgcc ggtcttcatc gcagccggca
    12781 gggtagctga ttgtgtactg aaataaccac caaaaggaca acctctccag caccggttcc
    12841 ggtactggca attggtccgg tcatgatgcg gctgggtcag gttggcactc cggccaataa
    12901 tcatatgacg gcttcctttg aaatgatttt taatcttagc cgccacatcc ttttccacgc
    12961 aattcaattc catggcgggc ataaagtctc cgtctggtaa aacctccagt ccttccaggc
    13021 t
//
LOCUS       DAOPMW010000055        12926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4973_length_12926_cov_6.992231, whole genome shotgun sequence.
ACCESSION   DAOPMW010000055 DAOPMW010000000
VERSION     DAOPMW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_12926_cov_6.992231"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /locus_tag="PLU11_04715"
     CDS             complement(<1..2458)
                     /locus_tag="PLU11_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58449.1"
                     /translation="MVMYLRTLLSAFIFCFSVAVFAQGNNNFHVRQYGTEDGLPSNGI
                     KGLQWDTATRFLWIATEAGIVRYNGMSFKSYTSEDNPHITNERILFLIRNNAGKIYTA
                     DNMGNLFFVNKNKLDFYKKQLIVGNPASNIITLSVSDKFYQQDQKITTGPYALQFDQV
                     SPVTDTSCLIFRNGVLVYFSISMVGAERLTLPDPGVRWIFKCGNKIFLADKNNNFWQF
                     NRSTRGFTKVILEQRGVPSGQDPDNSILIWDNGMEFPLLVNKEKIWKIKFENGVLYSE
                     LISDQVPQDVLIRYAQYDEKLNTLFLGTDSKGIIVIRENLVEPVRKVRSTTNERTSYY
                     SQVELPDGSIMTNEGHLLGKNNPTAPQALKGPYSISTYLQNDSVFWFIRTSKELGISC
                     LHKFNLNSGRLTPYPKIRPDYAQLVMAESGGQLYMANEVGIFRMNGDGIDTVYHYKSS
                     EQLRTHFDMKEISPGVLAIANCYALLQFDTKTNRLDTLYTDGNYCVRTIWTYKDYIFF
                     GTYGNGLYVSHKGLVKALPLDKNRHLLYTHCFVDDGKGFCWISTNRGLFKASIEDMIH
                     AYEKNDQQLYYHYFGRNDGMDMTEMNGGCMPCGLVMKNKTISFPTMEGLLWVNPATAI
                     PVLPDGEIFIDEVTVDDKRIDPATFDKESVNAFHRDVLIRLGFSAWCNKENIYLEYRL
                     NNERDWKMVNTDNGALIQLGNLESGEYTLNIRKLNGFGINNYSYKQYKFSVPAPWYQR
                     WWFYILSTGIVFGALSLYFRFRTRQYEIREQRLENQVSEKTKELQEQNEVLEKNNSIK
                     TRLISIISHDIVTPLKFLNVA"
     gene            complement(2487..3161)
                     /locus_tag="PLU11_04720"
     CDS             complement(2487..3161)
                     /locus_tag="PLU11_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58450.1"
                     /translation="MEISALMNDLINELPAVKLNPVMQPKILIADDHSMIRKGLKLHV
                     QLTLGFTDIEEVGTCNELMKELVKNKYTHLILDIILSDGNTLEVIPNIRRVYPDLNIL
                     VFSMQPAEIYGEAVKQYGINYYLSKSVGEDEMIQTLQKFIHNETPQKRTDIHHQDNPF
                     KTLAPRELEILHYVLKGIGTKEISENLNLKMNTVSTIKTRIYEKTNAGNIKELIELAT
                     LYNVNY"
     gene            complement(3200..5959)
                     /locus_tag="PLU11_04725"
     CDS             complement(3200..5959)
                     /locus_tag="PLU11_04725"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN58451.1"
                     /translation="MFPVLRYSLLLTGIFFLCFNTHAQPQKSNKGKEFWLGYGHNVLF
                     TQGGNSQNMVLYLSAEAAATVTISVNGTSWSQTVSVPANTVDATVIIPKTGPEDARIL
                     NEGLSTKGIHIISDTPIVVYAHQQGNVSSGATMLMPVETYGYTYYSLNFTQLSNYPDS
                     YSWFFVVAAENNTRLEITPSDSTEGGWLPNQIYTVDLNKGEIYNVFGKKTGNYTGKDM
                     SGSKIVSVSGADGNCHPVAVFSGSSRNIICSQGNGGEVMQQQIFPANAWGTRYLTYHT
                     VMNPNGDITGPFLNYYRVAVRKPGTVVKRNGIPLTGLINNFYYEFTSDSGDYIEADAP
                     ILVAQYAVSINECAGIIDPPLGDPEMLFLSPIEQGVKSARFYATRKEAIDITFINILI
                     PQTGLASLRIDGTAPNAAEYFTHPADPTYAVVVRRFLGAPVQHSISSDSSFIASVYGA
                     GYFESYGYNMGTLVNDLNSRSGYQNVFNTSGLADTMTCPNSPFRLSLKLAYKATRIHW
                     RLSQVTGLNPNTDSIQINPVPVDSSIINGRKYYHYTLQQDFTLARTGSYDIPVEYSAP
                     EIDACNQTEFTSIRFIVKEGPKPDFTISDPACLADNILFTGTSNTNGFSISNYLWRFA
                     DNTTANTQNTSKQFTTAGNQAIQYQLVTTNGCIGDTSKIITIHPNPVADFTISGAPFC
                     TEKELTIRSSLGGISQWNWDLGNGSSTAVPSFMHQYATAGNYTIRLITANAAGCLSVP
                     FQQSLQVNPTPLVNAGPDTVIRAGTSISLPATVSPAGNYQYTWTPATYLSSASVLSPV
                     CTPVLSTQYLLQVIETGTGCAADDEVLVTAVAGLFIPTGFTPNGDGKNDAWTIPGLAL
                     YPGARVAVFNRTGQLLFESSDYTRHPWDGRYRGQVQPTGVYIYLVELKDNSRQILKGT
                     LSLIR"
     gene            complement(5987..9586)
                     /locus_tag="PLU11_04730"
     CDS             complement(5987..9586)
                     /locus_tag="PLU11_04730"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58452.1"
                     /translation="MRKPLTFVSVLLLLLCGSFLHAQDKSNKGKEFWLSYGYHWTFDA
                     EGPPFNTQDLVLYLSTEAAATVTVSVTNTTWSQTVNIPANTVNASIMIPKTGADDARI
                     FNEGLLNKAVHIVSDTAIVVYAHSYNTMTSAATMLMPVETYGYKYFSLNYSQSTSGFS
                     PPTSTSTTTQNGPAWYSWFYVIASEDYTRLQITPSDTTRNGWLSTQTYTVNLMKGEIY
                     NVMGKLSGGNQLWAASKDLTGSKVVSVPGADGVCHPIAMFSGSSGIRLCRGDGGEVMQ
                     QQVFPTQAWGTRYLTYHMLNNTSTDINEPFKNFYRVAVEDPSTIVKRNGTVLTGLINN
                     FYYEFLDSLGGDFIEADKPILMAQYTPGGNRCYGSSQTAYGDPEMFYLSPLEQGRKDV
                     LFYATRKSTIDYNYVHVIVPTAGLNSLRLDNIAFDPLNTRTHPNHPGYSVAVARIPGA
                     AAQHRLTCDSIFTGTMYGIGYFESYGYNVGTFINNLNYYSEIKNTFSSTTTDSFTCPK
                     TPVRLFVKVGYPATRIHWKLSQVPGMTPATDSIIVNPVPVRTELLNGRNYFVYTLQQD
                     FSFATSGTYYIPVSYSATVIENCNQTENAQVKVVVRPGPKADFTFPSPSCLINTIPFT
                     GTSVPGIFNINNYTWFFHDNTTANTVNATKLYPAPGNYDVRYRILSDNGCAGDTTKTV
                     TILENPTAAFSISGNICTKDSVRITDASTVPVGTISSWYWNFGDGNSSTLTNNTPFYH
                     QYNTAGTYTIYLVTTSSNGCKSDTTRIPVTVLPRPTASFTTGGNVCLGDSIRFIDAST
                     PAASISSWNWNFGDTRSEIRTNNTPFFHTYTSTGNFNVTLVVTGTNTCASDTFRFTVN
                     VSSKPPSTFTVTGKPCTDSLFSFTSSVPTGGANPPTWYWNFGDNQTGNSSVSNSITHA
                     YANAATGIVVKHWVSYAAGCASDTAFMNLPVIIANPVASFSIIGDTLCEKKPITLTST
                     VTGTHFWNWSLGPTTTNATPPFTHQFTTAGTYDIRLFLSNPEGCKSAPVSQSITINPI
                     PAINAGPDKMINAGSSTTIDASITNPGNYSFIWTPSIYLDAATILNPVSTPDNTTTYT
                     IQAFDKVSNCIGTDEVIISPITGLYIPSAFTPNGDGKNDNWQIPGLALYPDAVVTVFN
                     RAGQVIFNQKGYTTSPWDGTLKGTRQPIAVYVYVIRLNDTANRVLKGTVSIIR"
     gene            9717..>12926
                     /locus_tag="PLU11_04735"
     CDS             9717..>12926
                     /locus_tag="PLU11_04735"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPN58453.1"
                     /translation="MKKSCFGKILRYLPVILFFLSSLLASGQQSFYDIKNWRFANPRQ
                     FGFNVIDIDFVNDTRGVAVGANGGIAYTNDGGTTWNYANFSFIDRTGIQASTSFNDVH
                     FASANVVYAVGSAGAMAKSTDGGANWSFVRTTFYNTGRTINAVWFLDDNKGYIGGQWN
                     AVDSIPRLYVTNNGGATWDSLVSPIGGKSVYGYVNNTSYAPIVADITPKAKEITRIEF
                     LNPTTGYITGTTASGGGNYFPRHPAVNTTTCVPNNTTTTTNAGDASLVWKFTNGSLTD
                     YSVSKERLGYNGIYNTAPNCSYRFASNTIHTQTYRAMNIINDSTLLIASFNNNIIIRI
                     NTGKNDSTLNINVPGVYEKGKYTLLNAPSPPTNNSNVLGAPIPANPVFAMSNPTNMMR
                     AANGKLFVPVLSPTIGPTNRMWTSTNNGVNWTEERWLPTGRKYSQYGGAAMDILPSGR
                     IVIAGQAGVVADSVSGSPWRSNYEVDAVGSYNKIDFADCNNGMAAGTSVIAITNNAGK
                     TWTEFIRTDFQALNISINSGTYVPGNPSTAYFATSTGTIYKSTNINTPAAVMDPMYAN
                     PLDQVWDVATVGNDSVWACGYTGFSVPTANRVPKVYRSFNGGLTWSVYSNFGVGSLFQ
                     NFRHIEFPTNTIGYVAGTRDTIWKTTDAGVTWNKLPLPFPGVTPQITYNDMFALDANT
                     VFLVGNGFPRKVIIRSLDGGNTWQDITGNALAIFPVGNFNSVVFHDANNGYVGCAGGF
                     LVTNNGGVTWRLDFPASGSNQISMAFAPKKVPAGIAFANRKLFTVTAFSNNILEYGDR
                     ANVDVNSTEVIGNATCTNLSGGSVTVNATGGLAPYTFSADGGAFQSSNVLTGLTQGAH
                     TITIKDAFCGTVTKTVNIGFTDNLVVNTIPAVDTLVCSGSPVPLLASTNGTGASYTWV
                     PIGGLSNVNIANPVAILNQDITYTVTATLNGCIRSKSVNIRIKPLPSISAGQDKTVVE
                     GDSFTFEGTGPGGTQLISWTPSTGMTGANTYTPSVRPNATTTYTMTVRDNASCTSSDN
                     VVITVLPYCLKVMDAFTPNGDGQNDTWVVTNNGGSCTKQV"
BASE COUNT         3312 a         2881 c         3179 g         3554 t
ORIGIN      
        1 cggccacgtt gaggaatttc aacggggtca cgatatcatg actgataatg gagatgaggc
       61 gtgtcttgat gctgttgttt ttttcgagta cttcattttg ttcctgcagc tcttttgttt
      121 tttcagaaac ctgattttcg agtctttgtt cccttatttc atactggcgg gtccggaacc
      181 gaaaataaag actcagggcc ccaaatacaa ttcctgtact caggatataa aaccaccagc
      241 gctggtacca gggagccggg acggaaaact tatattgttt atatgaatag ttattgatac
      301 caaacccatt cagcttgcgg atattcagcg tgtattcacc actctccagg ttacccagct
      361 gaatcagcgc cccattatct gtattcacca ttttccagtc ccgctcatta ttcagccggt
      421 attcgaggta aatattttct ttattgcacc aggctgaaaa acctaagcgg atcagcacat
      481 cccggtgaaa agcgtttaca ctttctttat caaaggtggc cgggtctatc cgcttatcat
      541 caacagttac ctcgtcaata aatatttcac catcgggcag tacgggtatt gcagtggcag
      601 gatttaccca taacaagccc tccatggtgg gaaaggaaat ggttttattt ttcattacca
      661 gtccgcaagg catacagccg ccattcatct ctgtcatatc catgccgtca ttccgtccaa
      721 aatagtgata atacaactgt tgatcattct tttcgtaggc gtgtatcatg tcttcaatac
      781 tggctttgaa taagccccgg ttggtactga tccaacagaa gccctttccg tcgtcaacaa
      841 aacagtgggt gtataataaa tgccggtttt tatccagcgg cagggccttt accaggcctt
      901 tatgactgac atacagcccg tttccatagg tgccgaagaa tatataatcc ttataggtcc
      961 agatcgtacg tacacaataa ttgccatcgg tataaagcgt atcgagcctg ttggtctttg
     1021 tatcaaactg caataaggca tagcagttgg caatggccaa cacgcccgga ctgatttcct
     1081 tcatatcaaa atgggtcctc agttgttccg aacttttgta atgatagacg gtatcgatac
     1141 catctccgtt catcctgaaa atacccactt cattggccat atataattgc ccgccggatt
     1201 cagccatgac cagctgtgca tagtctggtc ttattttggg gtaaggagtc aggcggccac
     1261 tgttgagatt gaatttatgc agacaggaga tgcccaattc tttgctcgtc cgtataaacc
     1321 agaaaacaga atcattttgc agataggtac tgatgctgta tggacctttg agtgcctgtg
     1381 gggcggtggg attgtttttt cccagaagat gcccttcatt ggtcatgata ctgccatccg
     1441 ggagttccac ctgtgaatag taggaggtac gttcattggt tgttgacctt acttttcgca
     1501 ccggctccac caggttctcc cggatcacga taattccttt tgaatcagta cccaggaaca
     1561 gggtattcag tttttcgtcg tattgcgcat aacggatcag tacatcctgt ggtacctggt
     1621 cactgattag ttcggagtag agcaccccgt tctcaaattt gattttccag attttttcct
     1681 tgtttaccaa cagcgggaat tccatcccgt tatcccatat caggattgaa ttgtccggat
     1741 cctgaccgga ggggacaccc cgttgttcca gtatcacttt agtaaaaccc cgggtggatc
     1801 tattaaactg ccagaaatta ttattcttat cagccagaaa aattttattt ccacatttaa
     1861 aaatccatct taccccggga tcaggaagtg tcagtcgctc cgcacctacc atggaaatac
     1921 tgaaatagac cagcacaccg ttccggaaga tcaggcagga ggtatctgtt accggactaa
     1981 cctggtcaaa ctgcagggca taaggtccgg tggtaatttt ctggtcctgc tggtaaaatt
     2041 tatcagatac actgagggtg ataatattac tggcggggtt cccgactatc agctgctttt
     2101 tataaaaatc aagcttgttt ttattgacaa aaaagagatt acccatatta tcagcggtgt
     2161 aaatcttacc ggcattgttc cggatcagga aaaggatccg ttcgttggtg atatgggggt
     2221 tgtcctcgct ggtgtatgat ttaaacgaca tcccgttgta ccggacaatc ccggcttcgg
     2281 tcgcaatcca gagaaagcgg gtggcagtat cccattgcaa tcctttgatg ccgttcgaag
     2341 gcagtccgtc ctcggtacca tactggcgga catgaaaatt attatttccc tgggcaaata
     2401 cagctacaga aaaacaaaaa ataaaggcag ataacaatgt tctcagatac ataaccatgg
     2461 ctccggaaca agttactgat tcctgattaa taattgacgt tgtacagggt ggccagctcg
     2521 atcagctctt ttatattgcc ggcattggtt ttttcgtaga tccgggtctt gatggtggac
     2581 accgtattca tcttgaggtt caggttctcc gaaatctctt tcgtaccaat accttttaag
     2641 acatagtgca ggatctcgag ttcacggggc gccagggtct tgaagggatt atcctggtga
     2701 tggatatccg tgcgtttctg cggggtttcg ttatggatga atttctgcag ggtctggatc
     2761 atctcatctt ctcccaccga tttggagaga taataattaa tgccgtattg tttcaccgcc
     2821 tcaccataga tctcggccgg ctgcatggaa aagaccagga tattcagatc aggatacacc
     2881 cggcggatat tcggaatgac ttcgagggta ttgccgtcgg aaagaatgat atccagaatc
     2941 aggtgggtgt atttattctt caccaattcc ttcatcagtt cattgcaggt gcctacttct
     3001 tcaatatcgg taaagcccag tgtcaactgg acatgcagct tcagtccttt gcggatcatg
     3061 ctgtgatcat cggcgatgag aatttttggc tgcattacag gattcagttt tacggcgggc
     3121 aattcgttga tcaggtcgtt catcaaggca ctaatttcca tcttttcttt tggctttttg
     3181 tagaattaat tctataaact tatcgtatca ggctgagcgt ccctttcagg atctgccggc
     3241 tattatcttt taactcaact aagtatatat acacacccgt gggctgtacc tgtcccctgt
     3301 atcggccatc ccaggggtgg cgggtataat cgctgctttc aaataacagc tgtccggtcc
     3361 ggttaaacac cgccacccgt gcaccggggt agagggccag tccggggatg gtccaggcat
     3421 catttttccc atccccattg ggggtaaagc ccgtgggaat gaacaaacca gctacggcgg
     3481 ttaccagcac ctcatcatcg gccgcacaac cagtgccggt ttcgatcacc tgtaataagt
     3541 attgagtact taaaacaggg gtgcagaccg ggctgagcac cgaagccgaa ctgagataag
     3601 tagcgggcgt ccaggtatac tgataattac ctgctggtga tacggtggca ggcagactga
     3661 tcgatgtgcc ggcacggata accgtatcgg gtccggcatt gactagcgga gtgggattca
     3721 cctgcaggct ttgctggaac gggaccgata aacaaccggc cgcattggct gtaatcaaac
     3781 ggatggtata gtttccggcg gtagcatatt gatgcataaa ggaaggtaca gccgtactgc
     3841 tgccattgcc cagatcccag ttccactgac tgataccacc cagggaggag cggatggtca
     3901 gttctttttc cgtacagaaa ggagcgccac tgatggtaaa gtcagcgacc gggttgggat
     3961 ggatcgtgat gatcttgctg gtatcaccga tacaaccatt ggtagtaaca agctggtatt
     4021 gaatagcctg attaccggct gtggtaaatt gtttggaagt gttttgtgta ttggcggttg
     4081 tgttatctgc aaaacgccat aaataattgc tgatcgaaaa tccatttgta ttggatgtac
     4141 cggtgaatag tatattatca gccaggcagg ccggatcgct tatcgtaaaa tcaggtttgg
     4201 gtccttcttt gacgatgaag cggatgctgg taaactcggt ttgattacag gcatcaattt
     4261 ccggagcgga atactctacc gggatatcat agctgccagt acgggccagg gtgaaatcct
     4321 gttgcagggt ataatgataa tattttcttc cattaatgat ggatgaatcc accggcaccg
     4381 ggttgatttg tatcgaatcg gtattcggat ttaatccggt tacctggctc aggcgccagt
     4441 gaatacgggt ggctttatag gccagtttca gggagagacg aaagggagaa ttcggacagg
     4501 tcatggtatc cgccaatccg gatgtattga atacattctg gtaacccgac cggctattga
     4561 ggtcgttcac cagggtgccc atattatagc catagctttc gaagtagccg gctccatata
     4621 ccgatgcaat aaaggaggag tcgctgctga tgctgtgttg taccggggcg ccaaggaaac
     4681 ggcgtactac aacggcatag gtgggatcgg caggatgcgt aaaatattcc gcggcattag
     4741 gtgctgtgcc atcaatccgt aatgaggcca ggccggtttg cggaatcagg atattgataa
     4801 aagtaatatc aatcgcttct tttcgggtgg cataaaagcg ggctgatttt acaccctgct
     4861 cgataggact cagaaaaagc atttccggat cgcccagggg cggatcgatg ataccggcgc
     4921 attcattgat tgacaccgca tactgggcca caagaatggg ggcatcggct tcgatataat
     4981 caccgctgtc actggtgaat tcgtaataga agttattgat gagtccggtt aaaggaatac
     5041 catttcgttt taccacagta cccggtttac gtaccgccac ccggtaataa ttcagaaagg
     5101 gaccagtgat atcgccattt ggattcatga cggtatgata agtgagataa cgcgtacccc
     5161 aggcgtttgc agggaaaatc tgttgttgca tcacttcgcc tccattgcct tgtgagcaga
     5221 tgatattgcg gctgctgccg gagaataccg ctacgggatg acagttgcca tcggcacccg
     5281 atacagagac gattttactg ccgctcatat ccttgccggt atagtttcct gtttttttac
     5341 caaagacatt ataaatctcg cctttgttca agtcgacagt atagatctga ttcggtaacc
     5401 agcccccttc ggtagagtcg gaaggcgtga tttctaaacg ggtattattt tccgcagcca
     5461 ctacaaaaaa ccaggagtaa ctatccggat aattggagag ctgggtgaag ttgagggagt
     5521 aataggtgta accgtatgtt tccaccggca tcagcatggt ggcaccggag gaaacatttc
     5581 cctgctggtg ggcatagact acgatcggag tatcactgat gatatgaatg ccttttgttg
     5641 aaagtccttc attcagtata cgggcatctt caggaccggt tttgggaatg attacggttg
     5701 catccacagt gttggcaggt actgaaaccg tctggctcca ggaagtacca tttacggaaa
     5761 tcgttacagt ggctgcagct tcggcactca ggtagagcac catgttttgg gaattgcctc
     5821 cctgggtaaa taagacattg tgtccgtatc ccagccagaa ctcttttccc ttatttgatt
     5881 tttgcggttg tgcgtgggtg ttgaaacaaa ggaagaagat ccctgtcagc aaaagactat
     5941 atcggagaac agggaacatg cgtaaataaa ataggttgac tccgcattaa cggataatgc
     6001 tcacggttcc tttcagcacc cgattggcgg tatcattcag ccgtatcaca tatacataga
     6061 ccgctatcgg ctgacgcgtt ccttttaaag ttccatccca gggactggtt gtatatcctt
     6121 tctgattaaa gatcacctgt ccggcccggt taaatacggt cactactgca tccggataaa
     6181 gagcgagccc cggaatctgc cagttatcat ttttaccatc tccattcgga gtaaaagcag
     6241 aaggaatata cagaccggta ataggactaa tgattacttc atccgtaccg atacagttcg
     6301 atactttatc aaaagcctga atggtatacg tggtggtatt atccggtgtg gaaacaggat
     6361 tcaggattgt agccgcatca agataaatgg aaggcgtcca gatgaaagaa taatttccgg
     6421 ggttggtgat agacgcgtca atcgtggtcg atgatcccgc attgatcatt ttatccgggc
     6481 cggcattaat agctggaatc gggttgatcg tgatggactg actcaccggg gccgatttac
     6541 atccttccgg attggaaaga aagagtcgga tatcgtaagt gcctgcagtc gtaaactggt
     6601 gcgtgaaggg cggggtggca ttggttgtcg tgggccccag gctccagttc cagaaatgcg
     6661 taccggtcac tgtggaagtc agcgtaatcg gcttcttttc acaaagcgta tcgccaatga
     6721 tactaaagga agcaaccggg ttggcaataa tcaccggcag gttcatgaaa gcggtatcgc
     6781 tggcacagcc tgctgcataa ctgacccaat gtttcaccac aattcccgta gctgcattgg
     6841 cataggcgtg ggtaatgctg ttcgatacgg aggagttacc ggtctggtta tctccgaaat
     6901 tccaatacca ggtaggagga ttagccccac cggtgggtac ggaagaagta aaactgaata
     6961 aactatctgt acatggtttt cctgtaactg taaaagtgga gggtggttta ctgctcacat
     7021 tcacggtgaa gcgaaaggtg tcgctggcgc aggtattcgt gcccgttact accagggtca
     7081 cattaaaatt acccgtgctc gtataagtat gaaagaaagg cgtattgttg gtccgtatct
     7141 ccgaacgcgt atcaccaaag ttccagttcc agctgctgat cgatgcggcc ggtgtggagg
     7201 catcgatgaa gcggatcgaa tcaccaagac agacatttcc ccccgtagtg aaactggctg
     7261 ttggtcttgg taagaccgtt acagggatac gggtggtatc actcttacaa ccattggagg
     7321 aggtagtcac caggtaaatg gtataagtac ctgctgtatt gtattgatga tagaaaggcg
     7381 tgttgttagt gagggtactg ctgttgccat ctccgaagtt ccaataccag ctgctgatcg
     7441 tacccaccgg aacggttgag gcatcggtaa tgcgaaccga atctttggta cagatattcc
     7501 cgctgatact aaaagccgca gtggggtttt ccagtatggt tactgtttta gtggtatcac
     7561 cggcgcatcc gttgtcggaa agaatgcgat accgtacatc atagttacca ggagccggat
     7621 agagcttggt agcatttacc gtattggccg tggtgttatc atggaagaac caggtgtagt
     7681 tgttgatatt aaatatgccg ggaaccgaag tgccggtaaa gggaatcgta ttgatcaggc
     7741 aggaaggtga ggggaaggtg aaatcagctt ttggtccggg gcgtactact acttttacct
     7801 gggcattttc ggtctggttg cagttttcga tcactgttgc tgaataagaa accgggatgt
     7861 agtaggtgcc ggatgtggca aaactgaaat cctgttgtaa cgtatataca aaatagtttc
     7921 ttccgttcag tagttcagtg cgaaccggta cagggtttac tatgattgaa tctgtggcag
     7981 gtgtcattcc cggtacctgg ctgagtttcc agtgaatgcg ggtggctgga tagcctactt
     8041 taacaaaaag tcgtacaggt gttttaggac aggtaaatga gtcagtggtt gtgctgctga
     8101 atgtattttt tatttcactg taataattca ggttattgat aaaggttccc acattgtagc
     8161 cataactttc aaaataacca atcccataca tcgtaccggt aaatatggaa tcgcaggtga
     8221 gccggtgctg ggcagcggcc ccgggaatac gggctacggc cactgaataa ccgggatgat
     8281 tcggatgtgt acgggtgttc aacggatcaa aggcgatatt gtccagtcgc agggaattca
     8341 ggccagcggt aggtacaatt acatgaacat agttataatc aatggtactc ttacgggtgg
     8401 catagaagag cacatctttt cttccctgtt ccagcggact caggtaaaac atttcgggat
     8461 cgccataagc ggtctgactc gaaccatagc accggttacc tccgggtgtg tactgggcca
     8521 tcaggatcgg tttgtctgct tcaataaaat cgccgccaag actatcgaga aattcgtagt
     8581 aaaagttatt gatcagtcct gttaaaaccg ttccgtttct ttttacaata gtcgacggat
     8641 cttctaccgc cacccggtaa aaattcttaa agggttcatt gatatcggtg ctggtattat
     8701 tcagcatgtg ataagtgaga tatcgggtac cccaggcctg ggtggggaat acctgttgtt
     8761 gcatgacttc accaccatcg ccgcggcata gtcggatacc actgcttccg gagaacatgg
     8821 cgatcgggtg gcaaacgcca tcggcgccgg gaacggaaac cactttactt cctgtgagat
     8881 ctttactggc cgcccagagt tgattacctc cactcagctt gcccatcacg ttataaatct
     8941 cacccttcat caggttaacg gtataggtct gggtgctgag ccagccattt cgggtggtat
     9001 cggaaggagt gatttgtaag cgggtataat cttctgaagc aattacatag aaccaggaat
     9061 accaggccgg accattttgt gtagtggtgg aggtgctggt gggcggactg aatccggaag
     9121 tggattggga ataattcagg gagaaatatt tatagccata ggtctctacc ggcatcagca
     9181 tggtagcggc tgaggtcatg gtattatagg agtgggcgta aaccacaatc gcggtatcac
     9241 tcacaatatg cacggcttta ttcagcagtc cttcattgaa aatacgggca tcatccgctc
     9301 ctgtttttgg gatcatgatg cttgcattca cggtgttagc tggtatattg acggtctggc
     9361 tccaggtggt attggtcact gatacagtaa cagtggcggc ggcttcggta ctcaggtaga
     9421 gcaccagatc ctgtgtattg aagggtggtc cttcggcgtc gaaagtccaa tggtatccat
     9481 agctcagcca gaattccttg cctttgttgg atttatcctg ggcatgcagg aatgaaccgc
     9541 aaagcagtaa taaaaggaca cttacaaatg ttagtggttt tcgcattctt caatcaggat
     9601 tcaatattcc gtcaaatcga ttgctcaaac agtagaatta attctacaaa cagaattcat
     9661 tatcgggggt atccttgtcg ctcatttcat ttccaccaaa actaaattta actaaaatga
     9721 agaagagctg ctttgggaaa atcctgcgat accttccggt gatcctgttt ttcctttctt
     9781 ccctcctggc ttccggtcag cagagttttt acgatatcaa gaactggcgg tttgccaatc
     9841 cgagacaatt cggattcaat gtgatcgata ttgattttgt aaatgacacc cgaggcgtgg
     9901 ccgttggagc caatggcggt attgcttata caaatgatgg cggtaccacc tggaattatg
     9961 ccaacttctc ctttattgac cgtaccggta ttcaggcatc cacttcattt aatgatgtac
    10021 attttgcatc tgccaacgta gtgtatgcgg tgggttctgc gggtgccatg gctaaatcaa
    10081 cggatggtgg agcgaactgg agttttgtca gaacgacttt ttataatacc ggacgtacga
    10141 tcaatgcggt ttggttcctg gatgataata aagggtatat cggtgggcag tggaatgcgg
    10201 ttgattctat tccccgttta tatgtaacga ataatggcgg tgctacctgg gattcactgg
    10261 tgtcgcccat tggtggaaaa tctgtatatg gttatgtaaa taataccagt tatgcaccga
    10321 ttgtagcgga tatcacccct aaagcaaaag aaattacccg gattgaattc ctgaacccca
    10381 caaccggata tatcaccggg acaacggcca gtggcggcgg taactatttt ccccgtcacc
    10441 ctgctgttaa cactaccacc tgtgtaccca acaacactac cactactacc aatgcaggtg
    10501 acgccagcct ggtatggaaa tttacaaatg gttcgctgac cgactattcg gtcagtaaag
    10561 aaagactggg ttataatggt atatacaata cagcccctaa ctgttcttac cgttttgctt
    10621 ccaatacaat tcatacccag acttacaggg ctatgaatat catcaacgat tccacgttgc
    10681 tgattgcttc ctttaataat aatattataa tccgtatcaa tacgggtaaa aatgacagta
    10741 cgttaaatat caatgtgcca ggtgtatatg aaaaagggaa atatacactg ctcaatgcgc
    10801 catcaccccc tactaataac tcgaatgtac tgggagcacc gattccggcc aacccggtat
    10861 ttgccatgtc caaccccacc aatatgatgc gggcagcgaa tggtaaatta ttcgtaccgg
    10921 ttttatcacc gacaatcggt ccgactaacc gcatgtggac ctctaccaat aatggagtta
    10981 actggactga agaacgctgg ttgccgaccg gccgtaaata cagtcagtat ggtggagctg
    11041 ctatggatat ccttccttca ggacgtatag tgattgcagg acaagcaggt gtggttgcgg
    11101 attccgtatc aggaagtccc tggagaagta actatgaagt agatgcagta ggttcctata
    11161 ataaaattga ttttgccgat tgtaataatg gtatggcagc gggaacttcc gttattgcta
    11221 ttaccaataa tgccggtaaa acctggacgg agtttatccg taccgatttc caggccctga
    11281 atatttctat taattccggt acctatgtac ccggaaatcc aagcacggct tattttgcca
    11341 ccagtacggg taccatttat aaatcaacga atattaatac tccggctgct gtaatggatc
    11401 cgatgtatgc caatccgctg gatcaggtat gggatgtggc tacagtaggt aacgacagtg
    11461 tatgggcttg cggatatacc ggtttctctg taccgactgc gaaccgtgtt cctaaagttt
    11521 accgttcatt caacggcgga ctaacctgga gtgtttatag taatttcgga gttggttctt
    11581 tattccagaa tttccgtcat attgaattcc ccaccaatac catcggttat gtggcaggaa
    11641 caagagatac gatctggaaa accactgatg cgggtgtgac ctggaataaa ctcccgttgc
    11701 ctttccccgg tgtaacacct cagatcacgt ataatgatat gtttgccctg gatgccaata
    11761 cggtattcct ggtaggaaac ggattccccc gtaaagtcat tatccgttca ctggatggtg
    11821 gtaatacctg gcaggatatc acgggtaatg cactggctat tttccccgta ggtaatttca
    11881 actccgtggt tttccatgat gccaacaatg gttatgtggg ttgtgccggt ggcttcctcg
    11941 ttactaataa cggcggtgtt acctggagac tggatttccc tgccagcgga tccaaccaga
    12001 tcagcatggc ttttgctcct aagaaagtac cggccggtat tgcatttgcc aaccgcaaat
    12061 tgtttaccgt aacggctttc agtaataata ttcttgagta cggtgaccgt gccaatgtgg
    12121 atgtaaactc tactgaagta atcggcaacg ccacctgtac taacctgagt ggtggaagtg
    12181 taaccgtaaa cgcaacgggt ggattagctc cttatacgtt tagtgcggat ggtggtgctt
    12241 tccagagctc caatgtactg actggtctta cacaaggtgc tcataccatc acgatcaaag
    12301 atgcattttg tggtacggta actaaaacag tcaatatcgg tttcacagac aacctggtgg
    12361 ttaataccat tccggcagtt gataccctgg tttgttccgg ttccccggtt cccctgctcg
    12421 ccagtaccaa tggcacagga gccagttata cctgggtacc gattggtggt ctttcaaatg
    12481 tcaatattgc caatccggta gcgatcctta accaggatat cacgtataca gtgactgcca
    12541 cactcaacgg atgtatccgt tccaaatcag tgaatatccg gattaagccg ttgcccagca
    12601 tcagtgccgg acaagacaag accgttgtag aaggagattc ctttacgttt gaaggaaccg
    12661 gtcctggtgg aacgcagctg atttcctgga caccttccac cggaatgact ggtgcaaata
    12721 cctacactcc atcagtaaga cctaatgcaa ccactactta tacgatgacg gttcgcgata
    12781 atgccagctg tacctcttcc gataatgtgg tgatcaccgt attgccttat tgcctgaaag
    12841 taatggatgc ctttactccg aatggtgatg gacagaacga cacatgggtg gtgaccaata
    12901 atggtggttc ctgtaccaaa caggta
//
LOCUS       DAOPMW010000056        12904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_4987_length_12904_cov_7.394972, whole genome shotgun sequence.
ACCESSION   DAOPMW010000056 DAOPMW010000000
VERSION     DAOPMW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_12904_cov_7.394972"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLU11_04740"
     CDS             complement(<1..1325)
                     /locus_tag="PLU11_04740"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPN58454.1"
                     /translation="MKSSLPVFIVLVLLMSCGDKNSPPPPPTPPAALTIRSNWVDSRT
                     FDDLHYGASRQPVLKIVFSAPLMRTTVAGAISFTDPAGITVPNTLSYQQGDSVLLIQP
                     NSTLAGITKYQFKLTNSVKSAAGGSLSGSIDKVLVTQIDSSRKFPLLTDNELLDKIQQ
                     TTFKYFWDFAHPVSGLSRERNTSGDLVTSGGSGFGVMAIVTGINRGFITRAQGLARLQ
                     TMVSFLKNTAVKVKGAFPHWLNGNTGAIIPFSSNDNGADLVETSYLMMGLLTARQYFN
                     GVDAAEATLRSDINTLYNNVEWDWFRNGGQNTLFWHYSPDKGWAMNMQIRGWNECLIT
                     YVLAASSTTHGIPAVVYTNGWAANGGAGFVNGNSYYGYTLPLGPAQGGPLFLAQYSFL
                     GINPNGLTDSWANYATHTRNHTLINYEYCRRNQAAGVGYSDSVWGLTAS"
     gene            complement(1340..2815)
                     /locus_tag="PLU11_04745"
     CDS             complement(1340..2815)
                     /locus_tag="PLU11_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HPN58455.1"
                     /translation="MLKYSSLLLLAVLAFSCNNKPDNNENAPVSGTPFSTDGKKVISW
                     TTADSSDLRLSLTDTLTFKEKAQPFENEVIVFVDPQKTFQTYFGTGAAMTDASAETFY
                     KLPKDKQAEFLKAYFDKETGIGYTVARTNINSCDFSSDMYTYVQDGDKELKSFNIEHD
                     RKYKIPFIKEAMKAAGGKLNLFASPWSPPSWMKSNNDMLHGGKLKPEFYDSWAMYYTK
                     FIKEYEKEGIPMWGISVQNEPMATQRWESCIYTAEEERDFLKNHLGPTMHKEGLQDKK
                     IIVWDHNRDLIYQRARTYFDDPEAAKYAWGIGFHWYEDWSGGQPMYDNLRRVHEAYPD
                     KNIMFTEGCAESFNAARYNAWVLGEEYGRSMINDFNNGMVGFTDWNILLDETGGPNHV
                     QNFCFAPVHGDTRTGQLIYTNAYYYIGHFSKFIQPGAKRIISSPSRSQLLSTAFLNPD
                     GTVVVIVMNQGNNTSPVFLWINGKAAEAQVKAHSINTYIIK"
     gene            complement(2834..4267)
                     /locus_tag="PLU11_04750"
     CDS             complement(2834..4267)
                     /locus_tag="PLU11_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPN58456.1"
                     /translation="MKTRMSLSLFLLAGVVSMSSNCSKNNAGGNNNNSETVNAEYWIT
                     RSDQTATLQKQSTSFVFSTDANNNSIIEIDSTKTFQTVDGFGYSLTGGSAFLINKMDE
                     ASKNSLLNEFFGAGESSIGVSYIRISIGASDLDAAVFSYDDLAAGQTDLSLSNFSLSK
                     DTVDLIPVLKKILAINPNLKIMGSPWSAPVWMKDNGSSIGGSLKPEYYSVYAQYFVKY
                     IQAMAAKGINLHAITVQNEPQHGGNNPSMVMSALQQGEFVKNHLGPAFRNAGITTKIV
                     VWDHNCNNPNYPITIMDDPAAKAFVDGSAFHLYEGEIGAMSGVHAAHPDKNLYFTEQW
                     TGANGSFDGDLKWHIKNVIIGSMRNWSKVALEWNLASDPNYQPHTPGGCSECKGAVTI
                     SGSVASKNVSYYIVAHASKFVTPGSVRIESSTGSTVSNVVFIRPDGKKVMLVVNENGA
                     AVNVNLKYKGKWAVVSIPGNAVATYIW"
     gene            complement(4295..7582)
                     /locus_tag="PLU11_04755"
     CDS             complement(4295..7582)
                     /locus_tag="PLU11_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPN58457.1"
                     /translation="MLRTSTTALLCSLLLLYSCSRQKKRPLFTSLSSAQTHISFSNHV
                     PEKERFNILYYLYFYNGGGVAAGDINNDGLTDIFFSANQKGKNRLYLNKGNFEFEDIT
                     DKAGVAGQSDWSSGVTMADVNGDGLLDIYVSSVSSKFGLTGHNELFINNGNNRFTEKS
                     EAFGLAFSGLTTQSAFFDYDHDGDLDCYILNQSDRPHANVVDTASRRKYDPLSGDVLL
                     RNDLKATGRFTDVSKEAGIYQSNLGYGLGLSVADLNNDGWEDIYIGNDFHENDYCYFN
                     NGNGTFTENGAAHFGHYSRFSMGNDINDYNNDGQPDIITVDMLPPDEKTMKTYGSDEN
                     PDIYRFKLEKNGYQHQVSRNVLQQNNGNGNSFSDIALQAGISATDWSWAPLFADFDND
                     GRKDLFISSGIVKRPVDLDYIRFVSAQEVNKGLNYTDKFDKETIDAMPEGSSHPFFFR
                     NENEYTFRDMSEEWGTGNLKGFYNGSVYADFDNDGDLDMVINCLDAEATVLRNNSNPG
                     NQLGIRFSGDSMNRFGIGAKIYLWAGGQLQYQQLMTTRGFQSSVEPRLHFGLGEAVKA
                     DSILVVWPDQRMQLLKQLPANKSLVLEQNDATGHFDYSAFFPPAQTGYSDITSSMGNP
                     WRHKENEFNDFDIQYLIPHTLSARGPKTAVADVNGDGLDDIYVCSGKGESGVLMIQGA
                     DGRFSASLQPDFDLNKESEDVDAAFFDANGDSHPDLLVITGGNEILATSRQGEDRLYI
                     NDGNGNFKKKELAFPVQYESKSCIAVSDIDKDGDMDFFIGNLANQTGYGQPVNSYLYL
                     NRGDATFDMAERNTIFLQKAGMVTAAVFADVNKDSWPDLVITGEWMPLKIFINKKGVF
                     TETAIPGSTGLWQSLFAADVNGDGYTDLLAGNWGHNSKFRAGKNGPLKLFMKDFDNNG
                     TVEQILCYTIDKKEYTFLAKDELERSLPVLKKAYLNYSDVAGKTVQYMFYDLFKDYTT
                     LQAETLSSSCFINNGKGNFTRSDMADAAQTAPLMCMNLASGKEGVRILAAGNYYGVIP
                     YEGRYDAMQPLQFSFAKDGTAAGAHNIMNNFKGEARDIDWVRTGTGDSLLVIARNNGP
                     LVFLKKN"
     gene            complement(7712..8560)
                     /locus_tag="PLU11_04760"
     CDS             complement(7712..8560)
                     /locus_tag="PLU11_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPN58458.1"
                     /translation="MKKQIRKIGFAAGALLLLASCQKMSKPDMGSFPEDSNPPGGPLA
                     FYVAFDGTTTNPLMNAVDSIRANFAADNPLASVDGIKGKAVKGENKKFVKYAKPNDWA
                     KNAKSFTISFWYKSNGQTKNNTGTNGPEYIMSFKSSNGHWSGAQALIFLEGDNTACAI
                     KTMFVDKNNGDAWFTWEGGNQIAGMMNDAWHHIALVYNATTSVMTCYLDGAARPTTGN
                     WGTHGNLNMDESKITEMRIGSGPSNSIDTDDWLASTFKGSIDQIRMYTTALTATEVSS
                     LFTGKL"
     gene            complement(8629..10128)
                     /locus_tag="PLU11_04765"
     CDS             complement(8629..10128)
                     /locus_tag="PLU11_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN58459.1"
                     /translation="MRRNKFVWLMLAIAIAGTTFTGCKKFLDRKPLQASLEDLNQGGL
                     EGQVFGLYGSFLDRGAAYHGYSSIPWFGFHSFRGDDAMKGSSPADGADWGVIFDDFQY
                     QKSHWAPTIYWDQNYNLINKANTVLQIADSLGLTDPASKVNIAEAKFFRALTYFQLVR
                     VFGEVPKIDFRIYNANDSKKPKATVADIYALIDADLQAGVSDLPENWLNATGNSRFPG
                     RLTKYTAMGLLAKVKIYSQNWAATLGLCQQVINSGNYQLLSNYSNLFKVAGENSKESL
                     FEIQADLGANGVPDNGYEFGVEQGVRGSGDWDLGWGWNTPTDDLVNAYEAGDARKSAT
                     ILFSGQDDGYGKTVPAYPIIPRLYWNKKVYPEPAMQTYTGRRQNAWLNHSLLRYSDVL
                     LLAAEAANELGGGTNQTNATTWVNMIRNRAGLPSITFTSQAQMRAAIKQERRMELAME
                     GERFFDLVRWGDAVTVLGPSGYTNCHKYYPLPQTAIDFAGGVLIQNPCW"
     gene            complement(10159..>12904)
                     /locus_tag="PLU11_04770"
     CDS             complement(10159..>12904)
                     /locus_tag="PLU11_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN58460.1"
                     /translation="SARKIDEVVVIGYGQASKRDLTGSITKISGRDIADKPNVNPVAS
                     LQGKVAGLSVVNSGTPGQEPDIRIRGTNSLSSAGVKPLYLVDGIFNDNISFLNPNDIE
                     SIEILKDPSSLAIFGVRGANGVIAITTKKAKTGQVNINFNTTFGSKKLVDKIDMVNAA
                     EFKMLFEEEQDNLGITGTDRFDFTPWTGNTDWVDAMTQTGTMSMTNFSVSTATDKNKF
                     YLGLGYTDEEGIVQHERLRKMSLNLSDEVKVTKGIKLGFTLNTFRQRNPYGNARGLLF
                     QARRALPVTPVYNDAAGAYYNLAIQSSQMINPAMELNDNWDKELNYENRSVASVYADI
                     SFLKNLNWRSTFYADMSVVDGRSYRPIDSLYDPIGGTTFIHNSYNRTEVSQSTQRWNK
                     FQQDHILTFKKSYGDHGLTLTSGFTTYYNEYRGLFGSIKQSLTGLPIPNDKRFWYLDN
                     GFGDKSTRVASSGQWEKATVSGLFRALYNFKGKYLLNGSFRRDWSSAWRQDYGNQGDN
                     FYSVGAAWEVSKEGFFQNQNVFDYLKVKASFGVLGVQNTYGFDYPAYPSLATNSTAVF
                     GTIIVPVYTETYLADRNLHWEKVHGKEIGIEFDMLKRKLHGEVVYYHKKTVDLLALIS
                     AGAGLQTLTNIGDMRNRGLELSASYTHEVKKDFTVTVSGNMTTFDNKFLNSPFTSNPD
                     EQYPNRTVAGNPIGYFYGYVVEGVYQSYADKLASPTMNLGNSYGPGDLKYKDLNGDGV
                     VNTDDRTMIGNPTPDFIYGGNVTVKYKGLDLSVDMQGVYGGEIYRYWGSSELPYTKFN
                     YPKFKLNRWHGAGTSNWDPILGDDHSTNRLPSSYGIEDGSYFRIRNFQIGYNFNADML
                     ARMHIKSLRAFFNIQNLKTWKRNSGYSPEFGGSPTSFGIDTGNDPIPMIISGGINVNF
                     "
BASE COUNT         3350 a         3141 c         2827 g         3586 t
ORIGIN      
        1 tcactggccg taagtcccca cactgaatca ctgtacccta cgccggcagc ctggttacgg
       61 cggcaatatt cataattgat cagcgtatgg ttgcgtgtat gggttgcata attagcccag
      121 ctatcggtca gcccgttagg gttgatgcca aggaacgagt attgtgccag gaaaagagga
      181 cctccctggg ccggccccag cggtaatgta tatccgtaat atgaattacc gttcacaaac
      241 cctgccccgc cattagccgc ccagccattg gtatatacga ctgccggtat tccatgtgtg
      301 gtggacgaag ccgctaatac ataggtaatc aggcattcgt tccagccacg gatttgcatg
      361 ttcatcgccc agcctttatc gggactgtaa tgccagaaca gcgtattctg tcccccgttg
      421 cggaaccagt cccattccac attattatac agggtattga tatcagatcg cagggtggct
      481 tcagctgcat ccaccccgtt aaaatactgg cgggcggtca ataaacccat catcaggtaa
      541 gatgtttcca cgaggtcagc cccgttatca ttactgctga atggaataat cgcgccggtg
      601 tttccattca accaatgggg gaatgcccct tttactttaa cagctgtgtt tttcagaaaa
      661 gaaaccatcg tttgcagacg ggccagtccc tgtgcccggg taatgaaccc gcggttgatt
      721 ccggttacaa tggccatgac cccgaagcct gaaccaccgg aggtaaccag gtcaccagaa
      781 gtattacgtt cacgagacag tccgctcacc gggtgcgcaa aatcccagaa gtatttaaac
      841 gtggtttgtt gaattttatc aagcagttca ttatccgtca gtaaaggaaa tttcctggag
      901 gaatcaatct gtgtaacaag tactttatca atgctgccgg aaagggatcc ccctgctgca
      961 gatttaacag aattggtcag cttaaactgg tatttcgtaa taccagccag cgtcgaattg
     1021 ggttgtatca gtagtacgga atcgccttgt tgataggaaa gtgtattggg aactgtaatt
     1081 ccggcaggat ccgtgaaaga gatggcaccc gctacggttg tccgcatcaa cggggcggaa
     1141 aatacaattt tcagcactgg ttgacgggat gctccataat gaaggtcatc aaatgtccgg
     1201 ctgtccaccc agttgctgcg tatcgttaat gcagcgggtg gggtgggtgg cggaggaggt
     1261 gaattcttat ctccgcaact catcagtaaa acaagtacta taaatacagg caaggagctc
     1321 ttcatgatcg gatcccgggt tatttgatga tatacgtatt tattgaatga gcctttacct
     1381 gcgcttcagc tgctttccca ttgatccaga gaaataccgg cgaagtattg tttccctggt
     1441 tcatgacaat caccacaacc gttccgtccg gattaaggaa ggcggtggac aataactggc
     1501 tgcggctggg actgctgata atgcgtttgg cgcccggttg aatgaatttg gaaaagtgtc
     1561 cgatataata ataggcattg gtataaataa gctgacctgt gcgggtatcc ccgtgaacag
     1621 gggcaaaaca gaaattctgc acatggttgg gacctccggt ttcatccaac aggatattcc
     1681 agtccgtaaa tccgaccatc ccattattga agtcattaat catactgcgt ccatactctt
     1741 cccctaatac ccaggcattg taacgggctg cattgaaact ctccgcacaa ccttccgtaa
     1801 acatgatatt cttatcaggg tatgcttcat gcaccctgcg caggttatca tacatcggct
     1861 gaccgccact ccagtcctca taccagtgaa acccaattcc ccaggcatat ttagccgctt
     1921 caggatcatc aaaataagtg cgggcccgct gataaatgag atcacggtta tggtcccata
     1981 caatgatttt cttatcctgc agcccttctt tatgcatggt aggccccagg tgatttttca
     2041 gaaaatccct ttcttcttct gctgtataaa tacaactttc ccagcgttgt gtggccatgg
     2101 gttcattctg tacggagata ccccacatcg gtattccctc cttctcatac tctttgataa
     2161 actttgtata atacatggcc cagctgtcgt aaaattctgg tttcagttta ccgccatgca
     2221 gcatatcatt gttgctcttc atccaggagg gtgggctcca gggactggca aataaattca
     2281 gtttgccacc ggctgctttc atcgcttctt ttataaaggg gatcttatac ttccggtcat
     2341 gttcaatatt gaacgacttc agctctttgt ccccgtcctg aacataggtg tacatatcac
     2401 tgctgaaatc gcagctattg atattggttc tggctacggt atagccgata ccggtttctt
     2461 tatcaaaata ggctttcagg aattccgcct gtttgtcttt cggcagttta taaaaagttt
     2521 ctgcagaggc gtcagtcatg gccgcaccgg ttccaaaata ggtctggaag gttttttgcg
     2581 gatccacaaa aacgatgacc tcattttcaa atggctgcgc cttttcttta aaagtgaggg
     2641 tatccgtaag ggataaacgc agatcagaac tgtctgccgt ggtccagctg attacttttt
     2701 tcccatcggt tgagaaaggg gtgccggaaa ctggtgcatt ttcattattg tcaggcttat
     2761 tattacagga aaaagccagt acagccaaca ggagaagtga ggaatatttt aacatgactt
     2821 atgatttaat atattaccag atatatgttg ctacagcatt tccggggatg ctaaccaccg
     2881 cccatttgcc tttatatttc agatttacgt ttacagctgc cccgttttca ttcaccacca
     2941 gcatcacttt tttcccgtca ggcctgataa aaaccacatt ggaaaccgtt gaacccgttg
     3001 agctttctat acgaacggat ccgggtgtaa caaacttaga agcgtgggcc actatataat
     3061 aactcacatt tttacttgcc acagaaccgc tgatagttac agcacccttg cactcagaac
     3121 aaccacctgg agtatgtggc tggtaattgg ggtcactggc caggttccat tcaagtgcca
     3181 ctttgctcca gttgcgcatc gaaccaatga tcacattttt tatatgccac ttcaggtcgc
     3241 cgtcaaaact gccgttagca ccggtccatt gttctgtaaa ataaaggttc ttatccgggt
     3301 gagcagcgtg cacgcctgac atcgccccga tttctccttc atataagtga aatgcactcc
     3361 catctacaaa ggctttggct gcaggatcat ccattatcgt gatcgggtag ttcggattat
     3421 tacagttatg atcccatacc acaatttttg ttgtaatccc cgcattccgg aaagccggac
     3481 caaggtgatt ctttacaaac tccccctgct gtaaggcaga catcaccata ctggggttat
     3541 tgcctccatg ttgaggctca ttctgtacgg tgatggcatg caggtttatt ccttttgcag
     3601 ccattgcctg gatatatttc acaaaatact gagcataaac actgtaatac tccggcttca
     3661 ggctgccacc aatgctgctg ccattatctt tcatccagac aggtgcactc cagggagagc
     3721 ccatgatctt cagattcggg ttgatagcca ggatcttttt taagaccgga atcagatcca
     3781 ctgtgtcttt agacaaactg aagtttgaca aggagaggtc tgtctgacca gctgcaaggt
     3841 cgtcataact aaacacagct gcatccagat cggaggcccc gatactgatg cggatataac
     3901 ttacaccgat gctgctctcc ccggcaccaa aaaattcgtt cagcagtgaa ttcttagatg
     3961 cctcatccat tttattgatc aggaaagcac tacccccggt aagcgaatag ccaaacccat
     4021 ctaccgtctg aaaagttttt gtgctgtcga tttcgatgat ggaattatta ttagcatcag
     4081 tgctaaaaac gaatgaagtg gattgctttt gcagggtggc ggtctggtca gacctggtta
     4141 tccagtattc cgcgttcacc gtttcactgt tgttgttatt tccccctgcg ttattcttgg
     4201 agcaattaga cgacattgat accacccctg ccaggaggaa taaagaaagg ctcattctgg
     4261 ttttcatgat gtagatgtat tgaagtaaag ttgcctagtt tttctttaaa aatacaaggg
     4321 ggccattatt acgggctatc accaaaagtg agtcgccggt cccggtacgc acccagtcaa
     4381 tatcccttgc ttccccctta aaattgttca taatattatg cgcaccggct gcagtcccat
     4441 cctttgcaaa actgaactgc aggggctgca tggcatcata acggccctca taaggtatca
     4501 ccccgtagta gtttcctgcg gcaagaatac ggacaccctc cttgcctgat gccaggttca
     4561 tacacattaa tggtgctgtt tgtgcagcat ctgccatatc agaacgggta aaatttcctt
     4621 tcccgttatt gataaaacag gaagaggaca aggtttccgc ctgaagtgtt gtgtaatctt
     4681 taaaaagatc gtaaaacata tactgaacag ttttgccggc cacatcactg taattcaaat
     4741 aggccttttt taacaccggg agggatcgct ccagttcatc ttttgccagg aaggtatact
     4801 cctttttatc aatagtataa cacagaatct gctccactgt cccgttatta tcaaaatcct
     4861 tcataaatag tttcaggggg ccgttcttac cggcccggaa ttttgaatta tgcccccagt
     4921 taccggccag cagatccgtg tacccatcgc cgtttacatc tgcggcaaat aaactttgcc
     4981 agagaccggt ggaacccggg atcgcggttt cggtaaatac ccctttttta ttaataaata
     5041 ttttaagggg catccattca ccggttatca ccagatcggg ccagctgtct ttgttaacat
     5101 ctgcaaaaac agcagctgtc accattccgg ctttctgcag gaatatagta ttccgttcag
     5161 ccatgtcaaa tgtggcatct ccccggttta gatataaata ggagtttacc ggctgtccat
     5221 agccggtctg gtttgctaag ttgccaataa agaaatccat atccccgtcc ttatctatat
     5281 ctgaaacagc aatacaggat ttgctttcat actgtacagg gaatgccagt tctttcttct
     5341 taaaattgcc attcccgtca tttatgtata agcgatcttc tccctgccgg ctggttgcca
     5401 ggatttcatt ccccccggta atcaccaata aatcgggatg actgtcacca ttcgcatcaa
     5461 aaaaagccgc atccacatct tcgctttctt tattgagatc gaaatccggt tgcagggaag
     5521 cagaaaagcg gccatcggct ccctgtatca tcagtacccc ggattcacct tttccactgc
     5581 aaacatagat gtcatccaaa ccgtcaccgt tcacatccgc aacggccgtt ttggggcctc
     5641 gtgcggaaag cgtgtgagga ataaggtatt ggatatcaaa atcattgaat tcattttctt
     5701 tatgccgcca tggatttccc attgaggatg ttatatcact atagccggtt tgagcgggtg
     5761 ggaaaaaggc tgaataatca aaatgtccgg ttgcatcgtt ctgttcaagt accaggctct
     5821 tattggccgg caattgtttt aaaagttgca tccgctgatc gggccaaacc accaggatac
     5881 tgtctgcctt aacagcctcc cctagtccaa aatgaaggcg gggttccaca gatgactgaa
     5941 acccacgggt tgtcatcagt tgctggtatt gcagctggcc accggcccac aggtaaattt
     6001 tagcgccgat cccaaaccgg ttcatactgt caccggaaaa gcggataccc agctggttcc
     6061 ccggattgct gttattgcgt aaaacagtcg cttccgcatc aaggcaattg attaccatat
     6121 caaggtctcc gtcattgtca aaatccgcat atacggatcc gttataaaat cccttcagat
     6181 tgccggtgcc ccattcctcg ctcatatccc tgaacgtata ttcattttca ttccggaaaa
     6241 aaaagggatg cgaacttcct tccggcattg cgtcaatggt ttccttatcg aatttgtctg
     6301 tatagttcag ccccttattc acttcctggg ccgatacaaa ccgtatataa tccaggtcca
     6361 ccggcctttt tacaataccg ctggaaataa aaagatcttt ccggccatca ttatcgaaat
     6421 cggcaaacaa cggtgcccag ctccagtcag tcgcagaaat accagcctgc agcgctatat
     6481 cgctgaagct gtttccgttt ccattgtttt gctgtaaaac attgcgtgaa acctggtgct
     6541 ggtatccgtt tttctccagt ttgaaccggt agatatcagg attttcatca ctgccatatg
     6601 ttttcattgt cttttcatcc ggaggcagca tatccactgt tatgatatcc ggctggccgt
     6661 cattattata gtcattaata tcattgccca tgctgaaccg gctgtaatgc ccgaaatgag
     6721 ccgctccgtt ttctgtaaat gtgccattcc cgttattgaa ataacaatag tcattctcat
     6781 gaaagtcatt tccgatatag atgtcctccc acccgtcatt gttcaggtca gctacggaca
     6841 gcccaagacc atacccgaga ttactctggt aaataccggc ttcttttgaa acatccgtga
     6901 aacggccggt ggctttgagg tcattccgca gcaggacatc cccggaaagc ggatcatact
     6961 tacgccggct ggctgtatcc actacgttgg catgcggccg gtccgactga ttcaggatat
     7021 aacaatccag atcgccgtcg tggtcataat caaaaaaggc tgactgggtt gtcagtccgg
     7081 aaaaggctaa tccgaaagct tcacttttct ccgtaaaacg gttgttgcca ttattaataa
     7141 aaagttcgtt gtgccctgtt agaccgaatt tgctgcttac ggaactaaca taaatatcca
     7201 gcaaaccatc cccgtttaca tcagccattg taacaccgga actccagtca gattgaccgg
     7261 ccactccggc cttatctgta atatcttcaa actcaaaatt tcctttgttg agatagagcc
     7321 ggttcttgcc tttctggttg gcggaaaaaa agatatcggt gagtccgtca ttattaatgt
     7381 caccggcagc cactcccccg ccattataga aatacaggta gtacagaata ttaaaccgct
     7441 ccttttcagg aacgtgattt gaaaaactga tatgagtctg tgcagatgaa agggaggtaa
     7501 aaagggggcg tttcttttgc cggctacaac tgtacagcag caacaaggaa cataatagcg
     7561 ctgtcgtgga agttctcaac atatagtgca attcaaaata tgactaggcc aaaaaagcgt
     7621 aaaagggctg aaacaataaa ttgttcagcc cctttttgct tatgctctgc gattgccaaa
     7681 aaaaatattc agtccaaccg ctgatgtttc attacagctt gccggtaaac aggctggaca
     7741 cttcagtggc agtaagtgcg gtagtataca tacggatctg gtcaatactt cctttaaatg
     7801 tggaagccag ccaatcatcc gtgtcaatac tgtttgaagg acctgaacca atccgcattt
     7861 cagttatttt actttcatcc atattcaggt tgccatgtgt accccagttt ccggttgtgg
     7921 gccgggctgc tccatccagg taacaagtca ttacgctggt ggtggcatta tacacgaggg
     7981 cgatatgatg ccaggcgtcg ttcatcattc cggcaatctg gttaccacct tcccaggtaa
     8041 accaggcatc gccattgttc ttatccacaa acatagtctt aatagcgcat gcagtattat
     8101 ccccttccag gaaaatgagg gcctgtgccc ctgaccagtg accattgctg gatttgaagc
     8161 tcataatata ttccggacca ttggtacctg tattgttttt tgtctggcca ttgcttttat
     8221 accagaacga aatagtaaaa ctcttggcat ttttggccca atcgtttggt ttggcgtatt
     8281 tgacaaactt cttattctcg cctttcactg ctttcccttt aatgccatcc acagatgcca
     8341 gcggattgtc tgcagcaaag tttgcgcgaa tactatccac tgcattcatc aggggattag
     8401 ttgtagttcc gtcaaaagca acataaaaag ccagcggacc accgggaggg tttgaatcct
     8461 cggggaaaga gcccatatcc ggctttgaca ttttctggca ggaagccagc aacaatagag
     8521 cgccggcagc gaaaccaatt ttccttattt gttttttcat tttattaatt tttaatgatt
     8581 aatcaggaat caggggatga tcggatcatc ccctgagaag attatatctt accagcatgg
     8641 gttttgaata agtactccac ctgcaaagtc aatagctgtc tgaggcagcg gataatattt
     8701 atggcagttt gtatagccag acgggccaag tacagttact gcatcacccc atcttaccag
     8761 gtcaaagaac cgctcgcctt ccatagccag ttccatacgt ctttcctgct tgatcgcagc
     8821 acgcatctgg gcctgggaag taaacgtaat actgggtaat ccggcacggt tacgaatcat
     8881 attcacccag gtggttgcat tggtctggtt tgtcccgcca ccaagttcgt ttgcagcttc
     8941 agcggccagc agcagtacat cagaatagcg aagcaatgag tgattcagcc aggcattctg
     9001 acggcgacct gtataggtct gcatagcagg ttccgggtat actttcttat tccagtacaa
     9061 acgggggata atcgggtaag ccggaacggt tttaccataa ccatcatcct ggcctgaaaa
     9121 taagatagtg gctgatttac gtgcatcacc tgcttcgtaa gcatttacca gatcatctgt
     9181 tggtgtattc catccccaac ccagatccca gtctcctgat ccacgcactc cctgctctac
     9241 cccaaactca taaccgttat cgggcacgcc attggcaccc agatcagcct gaatttcaaa
     9301 aagggattct tttgagtttt cacctgctac cttgaacagg ttactataat tactcagtaa
     9361 ctggtaatta ccagagttga tcacttgttg acagagacca agcgtggccg cccagttctg
     9421 actatagatt ttaactttag ccagcaggcc cattgcagta tacttggtga ggcgccccgg
     9481 gaaacggctg ttgccagtgg cattaagcca gttttcagga agatcactca cgcctgcctg
     9541 cagatctgca tcgatcaggg catagatatc agcaactgtt gctttcggct ttttagaatc
     9601 attggcatta taaattctga aatcaatttt tggcacttca ccaaatacac ggaccagctg
     9661 gaagtaagtg agtgcgcgga agaacttggc ttcagcgata ttcactttgg atgccggatc
     9721 agtgagaccc agtgagtcag cgatctgcag tacggtgttg gctttattta tcaggttata
     9781 gttctgatcc caataaattg ttggagccca gtgtgatttc tgatactgga aatcatcaaa
     9841 aatcacgccc cagtcggcac catcagcagg gctacttcct ttcatggcat catccccgcg
     9901 gaaggaatgg aaaccaaacc atggaatact gctgtatccg tggtaagcag caccccggtc
     9961 aaggaatgag ccgtataaac caaagacctg cccttcaaga ccaccctgat tcagatcctc
    10021 cagggatgcc tgtaagggtt tgcggtcaag aaacttttta caccccgtaa atgttgtacc
    10081 agcaatggct attgccagca tcaaccatac aaatttgttt cttctcatat tattcgtttt
    10141 atagaattca atcgtttatt aaaaattgac attaatacct cctgaaataa tcatcggaat
    10201 cgggtcatta ccagtatcga taccaaatga tgtcggagaa cctccgaatt caggtgaata
    10261 accagagttg cgtttccagg tcttcagatt ctgtatgtta aagaatgccc gaagactttt
    10321 gatatgcatt ctcgccagca tgtcagcgtt gaaattatac ccgatctgga agttcctgat
    10381 acggaagtag ctgccatctt caataccata gctggaaggc aggcggttag tggagtggtc
    10441 atcgcccagg atcggatccc agtttgatgt accggcacca tgccagcggt tcagcttgaa
    10501 tttcggatag ttaaacttgg tataaggcaa ttctgaacta ccccagtaac ggtagatctc
    10561 accaccgtaa acgccttgca tatccacact cagatcaagc cctttatact ttacagtaac
    10621 gttaccgcca taaataaagt caggagtcgg gttaccgatc atcgtacggt catctgtatt
    10681 aaccacaccg tctccgttca ggtccttgta cttaagatcg ccgggaccat aactgttgcc
    10741 aaggttcata gtcggggatg ccagtttatc tgcataagac tggtacacgc cttccacaac
    10801 ataaccgtaa aaatagccga tcgggttgcc tgctacagta cggttggggt attgttcatc
    10861 cgggtttgag gtaaatggag aattcaggaa tttattgtca aatgtggtca tattaccgga
    10921 aactgttacc gtgaagtctt ttttgacttc atgagtgtag ctggcagaaa gttccagacc
    10981 acggttacgc atatcaccga tgttggtaag tgtttgcaga ccggcacctg cactgatcag
    11041 tgccagcaga tccacggttt tcttgtggta atagacaacc tcaccatgca gtttgcgctt
    11101 taacatgtca aactcaatac cgatctcttt accatgtact ttttcccaat gcaggttacg
    11161 gtcggccagg taagtttcag tgtacacagg cacgataata gtaccaaaca cagccgtgga
    11221 atttgtagcc agagaaggat aagcagggta gtcaaatcca tacgtattct gtacacccag
    11281 tacaccaaag ctggctttca ctttcaggta atcaaataca ttctggtttt ggaaaaatcc
    11341 ttctttacta acctcccagg ctgcacccac tgagtagaaa ttatcaccct ggttgccata
    11401 atcctggcgc catgctgatg accagtcacg gcggaaagac ccgttcagca ggtattttcc
    11461 tttgaaatta tacaatgcac ggaacagtcc tgatacagtg gctttttccc actgaccgga
    11521 agaagcaaca cgagtgctct tgtctccaaa tccgttgtcc agataccaga aacgtttgtc
    11581 attgggaatc ggcagaccgg tcagggattg tttgatagaa ccaaacaagc cgcggtattc
    11641 attataataa gtagtgaatc cacttgtcag ggtcaggcca tgatcgccat agctcttttt
    11701 gaaagtcagg atatgatcct gctggaattt gttccaacgc tgagtggatt gtgaaacttc
    11761 tgtacgatta taactgttgt gaataaacgt agttccgccg atcggatcgt acagactgtc
    11821 tatcgggcga tagctacggc catccaccac agacatatca gcatagaatg tagaacgcca
    11881 gttcaggttc ttcaggaaac tgatatcagc atatacactg gcaaccgaac ggttttcgta
    11941 attcagctct ttgtcccagt tgtcattcag ctccatggcc gggttgatca tttgtgaaga
    12001 ctgaatagcc aggttgtaat aggcaccggc agcatcattg tatacagggg taaccgggag
    12061 tgcacggcgg gcctgaaaga gcaggccgcg ggcattgccg taaggattac gttgacggaa
    12121 tgtattcagg gtaaaaccaa gtttaatccc ttttgtgact ttcacttcat cgctcaggtt
    12181 cagggacatt ttacgaaggc gctcatgctg cacaatacct tcctcatctg tatagccaag
    12241 acctaagtag aatttgttct tatcggtagc tgtgcttaca ctgaagttgg tcatggacat
    12301 ggtaccggtt tgtgtcatgg catccaccca gtccgtatta ccggtccagg gggtaaagtc
    12361 aaaacggtcc gtaccggtta tacccaggtt atcctgctct tcttcaaaaa gcattttaaa
    12421 ctctgctgca ttaaccatat caattttgtc taccagtttc tttgaaccga atgtggtatt
    12481 gaagttgata ttcacctggc cggttttggc tttcttggtt gtaatggcaa ttacaccgtt
    12541 tgcaccacgt accccaaaga tggccagtga ggaaggatcc ttcaggattt cgatagactc
    12601 aatatcattt ggattgagga aactgatatt atcattaaag ataccatcca ccagataaag
    12661 cggttttaca ccggcactgg ataatgagtt ggttccacgg atgcggatat caggttcctg
    12721 gccgggtgta ccggagttta ccacgctcag accagctact ttaccctgca gggaagcaac
    12781 cgggttaacg ttgggtttgt cggcaatatc acgtccactg atcttagtaa tagatccggt
    12841 caggtcgcgt ttgctggcct gtccataacc aatcaccacc acttcatcaa tcttcctggc
    12901 agat
//
LOCUS       DAOPMW010000057        12784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5060_length_12784_cov_8.451646, whole genome shotgun sequence.
ACCESSION   DAOPMW010000057 DAOPMW010000000
VERSION     DAOPMW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_12784_cov_8.451646"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="PLU11_04775"
     CDS             complement(<1..1674)
                     /locus_tag="PLU11_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPN58461.1"
                     /translation="MAIRKENRPKAAFSKITIGLASPDTILERSYGEILKPETINYRT
                     YKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGVSSKKLETIVYYERFVVIQPGIRTDKGQNMGDLLT
                     EEEYLDILDTLPKDNQYLPDDDPNKFIAKMGAEAVHDMLARIDLDQLSFDLRNAAATE
                     TSQQRKADALKRLSVVEAFRDANTRINNRPEWMVMQYIPVIPPELRPLVPLDGGRFAS
                     SDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAEG
                     GRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSARKLVDKKEAVVWDILENILKGHPVMLNRAPTLHRLSIQA
                     FQPKLIEGKAIQLHPLVTSAFNADFDGDQMAVHVPLSHAAILEAQLLMLASHNILNPQ
                     NGTPITLPSQDMVLGLYYITKGKKTTDTETVRGQGKIFYSAEEVIIAHNE"
     gene            complement(1815..5618)
                     /gene="rpoB"
                     /locus_tag="PLU11_04780"
     CDS             complement(1815..5618)
                     /gene="rpoB"
                     /locus_tag="PLU11_04780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPN58462.1"
                     /translation="MSSTKVNSRVDFGKIKHLAETPDLLEVQIQSFKEFFQLETTPDK
                     RNIEGLFRVFKDNFPITDTRNIFVLEFLDYFIDPPRYTIEECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGNIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSVH
                     PNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRSIGFETDKDILT
                     LFGMADEIKAEKKTLSKYVGKRLAARVLRTWVEDFVDEDTGEVVSIERNEIVMERDTV
                     LDEEAIETISEMEVASVFVQREDVGGDYAIIYNTLNKDTSNSELEAVQFIYRQLRGAD
                     APDNETARGIIDKLFFSDKRYDLGEVGRYKINRKLNVDLPLEKKTLTKDDIILIIKYL
                     VRLTNGKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTPV
                     DLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEVR
                     DVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINDMGFIETPYRKVHEGKVNMKELNF
                     LSAEEEDLAKIAQANTQLDEKGNFKDERIQSRETGDFPILDKNEVEYMDVAPNQIVGL
                     SASLIPFLEHDDANRALMGSNMQRQAVPLLRPDVPIVGTGLEGKAARDARIQIHSDGE
                     GVVEFVDANEIHVRYERDASEKLVSFEDDLRIYRLTKFIKTNQETCINLSPAVKKGQR
                     VKKGDFLTEGYATQNGELALGKNLKVAFMPWKGYNFEDAIVISERVVKEDMFTSVHIS
                     EYELEVRDTKLGEEELTPDIPNVSEEATKDLDENGIIRIGAQVKEGDILIGKITPKGE
                     SDPTPEEKLLRAIFGDKAGDAKDASLKAPNGVEGVVIGKKLFQRAKKDKNAKVREKAA
                     IEKLEKIHEKNETDLMEVLLEKLTTLLKDHVSASVSNNFGEVQIGKGAKFTEKNLRGL
                     DYANINPLGWTGDKKVDDQINTLLHNYNIKFNEELGRYKREKFNISIGDELPAGVLKL
                     AKVYLAVKRKLKVGDKMAGRHGNKGIVAKIVRHEDMPFLEDGTPVDVVLNPLGVPSRM
                     NLGQIYETILGWTGEKLGLKFATPIFDGASVDEIASYCKDANIPMYGHTYLYDGETGE
                     RFHQKATVGIIYVIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAYGASNILQELLTIKSDDIIGRAKTYESIVKGENVPRAGVPESFNVLVHELRGLGL
                     DLKFE"
     gene            5884..6852
                     /locus_tag="PLU11_04785"
     CDS             5884..6852
                     /locus_tag="PLU11_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPN58463.1"
                     /translation="MKMKEKLAGYLTLSRKERTGVILLGLLIISLFLLPPLLPVPGPP
                     PVSPADTAWIVAMKKLEQKEESYPQGVPSSREDPVRPPARAGYGYPARSGTPSLFNFD
                     PNTLREEGWQQLGLREKTIKTILNYLSKGGKFRKPEDLQRVYGLFPDEYERLRPYIRI
                     GEVTSSPLPAWTNEKRDFEKPAKVTADTRRVIVEINTADTSALIALPGIGSKLAQRII
                     SFREKLGGFYSVKQVAETFGLADSVYRKIEPLLKLEVKTWRKININTATAEELKVHPY
                     LKWNVINALLAYRKEHGSFRNTDELKQVMAVTDEIYGRIVPYLVTE"
     gene            complement(7356..7730)
                     /gene="rplL"
                     /locus_tag="PLU11_04790"
     CDS             complement(7356..7730)
                     /gene="rplL"
                     /locus_tag="PLU11_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPN58464.1"
                     /translation="MADVKALAESLVGLTVKEVQELADFLKTEYGIEPAAAAVVVASG
                     DGGGAAAAEEKTAFNVVLKSGGASKLNVVKIVKDLTGLGLKEAKDLVDGAPKNVKEGV
                     SKAEAEEIAAKLKEAGADVEIA"
     gene            complement(7882..8409)
                     /gene="rplJ"
                     /locus_tag="PLU11_04795"
     CDS             complement(7882..8409)
                     /gene="rplJ"
                     /locus_tag="PLU11_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPN58465.1"
                     /translation="MTKDQKNEVIELLKGKFAQYNNFYIADTESLSVAQVGKLRRACF
                     SKQVEMKVAKNTLIKKALESLDSEKYASMYDSLHKVTALMFSENPKEPALIISSFRKE
                     SNGDKPVLKAAFINGDVYMGDNNLKALTQIKTKNELIGEVIGLLQSPAKRVLAALLHH
                     HEQKAGGAEVAAAAE"
     gene            complement(8487..9182)
                     /gene="rplA"
                     /locus_tag="PLU11_04800"
     CDS             complement(8487..9182)
                     /gene="rplA"
                     /locus_tag="PLU11_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPN58466.1"
                     /translation="MAFISKKRKVADTKVDANKAYSLKEASTLVKTISTCKFDASVDL
                     HIRLGVDPKKADQAIRGTVSLPHGTGKTKKVLVLCPPDKEAEAKAAGADYVGLDEYVT
                     KIEGGWVDVDVIIATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVANAINDVKGGK
                     IAFKVDKVGIIHASIGRVSFTPEKIAENSLELLNAIIKAKPSSAKGTYLRGISMASTM
                     SPGIAIDTKAFVH"
     gene            complement(9233..9676)
                     /gene="rplK"
                     /locus_tag="PLU11_04805"
     CDS             complement(9233..9676)
                     /gene="rplK"
                     /locus_tag="PLU11_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPN58467.1"
                     /translation="MAKEISGFVKLQCKGGQANPAPPIGPALGSKGINIMEFCKQFNA
                     RTQDKMGKVLPVLITVYTDKSFDFVIKTPPAAIQLMEAAKIQKGSKESNRVKVGKVNW
                     EQVKAIAEDKMPDLNCFTIESAMKMVAGTARSMGLNVEGKAPWEN"
     gene            complement(9899..10480)
                     /gene="nusG"
                     /locus_tag="PLU11_04810"
     CDS             complement(9899..10480)
                     /gene="nusG"
                     /locus_tag="PLU11_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPN58468.1"
                     /translation="METTPNTENVKQAETKWYVLRVVSGKEKKVKEYLDKEISRGGWS
                     EIVKQVFLPVEKVYKVASGKKVMRERNYYPGYVMIEIADGKLNDDLRDIIKNTTNVIH
                     FLGKEDPIALRKAEVNKMLGKMDEMAEVGGMSMSEPFIVGETIKIIEGPFNDFNGVIE
                     EVNDEKKKLKVTVKIFGRSTPVELNYMQVEKIS"
     gene            complement(10507..10707)
                     /gene="secE"
                     /locus_tag="PLU11_04815"
     CDS             complement(10507..10707)
                     /gene="secE"
                     /locus_tag="PLU11_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPN58469.1"
                     /translation="MNKITTYFKESFKELTEKVSWPTWQQLQQSTMIVLAATIVITFI
                     VLAMDSAIGNGLKFVYNQLYKA"
     gene            complement(10721..10791)
                     /locus_tag="PLU11_04820"
     tRNA            complement(10721..10791)
                     /locus_tag="PLU11_04820"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10757..10759),aa:Trp,seq:cca)
     gene            complement(10990..12177)
                     /gene="tuf"
                     /locus_tag="PLU11_04825"
     CDS             complement(10990..12177)
                     /gene="tuf"
                     /locus_tag="PLU11_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPN58470.1"
                     /translation="MSKETFKRDKPHVNVGTIGHIDHGKTTLTAAITTILASKGMATA
                     KKYDEIDAAPEEKERGITINTAHVEYQTANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTKEHILLARQVGVPKIVVFMNKVDLVDDPELLELVEMEIRDLLTF
                     YGFDGANTPIIKGSAIKALEGDAEAVKQVEELMTAVDEYIPLPPRPVDQPFLMSVEDV
                     FSITGRGTVATGRIERGRIKVGEPVEIVGLIAEPLTSTCTGVEMFRKLLDEGEAGDNA
                     GLLLRGIEKTQIRRGMVIVKPGSITPHTDFKAEVYVLSKEEGGRHTPFFQKYRPQFYF
                     RTTDVTGEVELNAGTEMVMPGDNTSLNVKLIQPIAMEKGLKFAIREGGRTVGAGQVTE
                     IIK"
     gene            complement(12303..12374)
                     /locus_tag="PLU11_04830"
     tRNA            complement(12303..12374)
                     /locus_tag="PLU11_04830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12340..12342),aa:Thr,seq:ggt)
     gene            complement(12541..12613)
                     /locus_tag="PLU11_04835"
     tRNA            complement(12541..12613)
                     /locus_tag="PLU11_04835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12578..12580),aa:Gly,seq:tcc)
BASE COUNT         3099 a         3169 c         2864 g         3652 t
ORIGIN      
        1 ttcattgtgt gcaatgatca cttcttccgc cgagtagaaa atcttaccct ggccgcgaac
       61 ggtttcggta tcagtggttt tctttccttt tgtgatataa tacagaccaa gtaccatgtc
      121 ctgtgaaggc agggtgattg gcgtaccatt ctgcgggtta aggatgttat gagaagccag
      181 catcagcaat tgggcttcca ggatcgcggc atggctcagg ggcacgtgta ccgccatctg
      241 gtcaccatca aagtcggcgt tgaacgcgga cgttacgagc gggtgcaact ggatcgcttt
      301 accttcaatc agtttgggct ggaaggcctg gatagacaga cggtgaagcg tcggggcacg
      361 gttcagcatg acagggtgac ctttcagaat attttccagg atatcccata ccaccgcttc
      421 cttcttatcc accagtttac gggcagactt cacggtttta acgatacctc tttctatcag
      481 tttacggatg ataaacggtt tgaataattc agccgccata tcttttggaa gaccgcactc
      541 atgtaatttc agttcgggac ctaccacgat aacagaacga ccggaatagt ctacccgttt
      601 accgagcagg ttctgacgga aacgtccttg tttacctttc agcacatcac tcagtgattt
      661 cagggcacgt ccgccttctg ctttaacggc attggactta cggctgttat caaacagtga
      721 atccacggct tcctgcagca tacgtttttc gttacgcagg atcacctcag gagctttaat
      781 ttccaataac cttttcaaac ggttattccg gatgattacc cggcgataca ggtcattcag
      841 gtcagaagaa gcaaaacggc caccatccag gggaacgagg ggacgcagtt ccggcggaat
      901 caccgggata tattgcatca ccatccactc cggacggttg ttgattcttg tattggcatc
      961 acggaaagct tccaccacac tcaggcgttt cagcgcatcg gccttacgtt gttgggatgt
     1021 ttcagtggca gcggcattcc gcaggtcaaa tgataactga tcgagatcga tacgggccag
     1081 catatcatgc acggcttccg cacccatctt ggcgataaac ttattgggat catcgtccgg
     1141 caggtattgg ttatccttcg gtaaggtatc caggatatcc aggtattcct cttcagtaag
     1201 gaggtcaccc atattctggc ccttgtcagt acggataccc ggctgaatca ccacaaaacg
     1261 ttcgtagtaa acgatcgtct cgagtttctt agagctaacc cccaacaggt aaccgatttt
     1321 attcgggaga gatttgaaat accagatatg cacaaccggc actaccagtt tgatatgtcc
     1381 cattctttcg cgacgtactt tcttctctgt tacttccact ccgcaacgat cgcagacgat
     1441 gcctttataa cggatacgtt tgtatttgcc gcaggcacat tcataatctt taactggtcc
     1501 gaagattctt tcgcagaaca aaccatctct ttcgggtttg tacgtacggt agttgatcgt
     1561 ttcgggcttt aagatctcac cataacttct ttcgagaatg gtatccggag atgccaggcc
     1621 gatggtgatt tttgaaaaag ccgctttggg acgattttct tttctgatag ccataattta
     1681 ctttatagtt gttagaccct gattagttaa ttcaaaatga aaaagtcaaa agtaaaattc
     1741 ctttattcga acctgccgga taaatggatt gaatcttaca gcaggatttt taatttttac
     1801 ttttgatttt ttacttattc aaatttgaga tccagaccca gtcctctcag ttcatgcacc
     1861 agtacgttga acgattctgg tacaccggct ctaggtacgt tctccccttt cacaatagac
     1921 tcgtaagtct tcgcacgtcc gatgatatca tccgatttga tcgtaagcag ttcctggaga
     1981 atattggaag caccgtaggc ttcgagggcc cacacctcca tctcaccgaa acgctgacca
     2041 ccgaactggg ctttaccacc caacggctgc tgggtaatca gactgtacgg tccgatcgaa
     2101 cgggcgtgca ttttatcatc caccatgtgg tgcagtttga tcacatagat gatacccacg
     2161 gttgctttct ggtggaaacg ctccccggtc tccccatcat acaggtaggt gtgaccatac
     2221 atcgggatat tggcgtcttt acagtaagaa gcgatctcat ccacggaagc accatcaaag
     2281 atgggcgtcg cgaatttcaa tcccagtttc tcaccggtcc agccaaggat cgtttcatag
     2341 atctgtccga ggttcatacg gcttggtacc cccagcggat tcaataccac atccaccggt
     2401 gttccgtctt ccaggaacgg catgtcttcg tgacgaacaa tcttggccac gatccctttg
     2461 ttaccgtgac ggcccgccat cttatcaccc actttcagtt tacgtttcac cgcgaggtat
     2521 actttcgcga gtttcagtac accggcaggt aattcatccc cgatactgat gttgaacttc
     2581 tcccgcttgt aacgccccag ttcttcgttg aacttgatat tgtagttgtg gagcagggta
     2641 ttaatctgat catccacttt tttatcaccg gtccagccca gcggattgat gtttgcgtaa
     2701 tccagtccgc gcaggttttt ctctgtaaac ttggcacctt taccgatctg tacttcaccg
     2761 aagttgttgc tcacactggc ggatacatga tccttcagca gggtggtcag tttttccagc
     2821 agtacttcca tcaggtcggt ctcgttcttc tcgtggatct tttccagttt ctcgattgca
     2881 gccttctcac gcactttcgc gttcttgtct ttcttggcgc gctggaaaag ttttttacca
     2941 atcaccaccc cttctactcc attcggtgct ttcagggaag catcttttgc atcaccggct
     3001 ttatcaccga agatggcgcg aagcagtttc tcttccggag ttggatcact ttctcctttc
     3061 ggggtgatct taccgatcag aatatcacct tctttcacct gggcaccgat gcggataata
     3121 ccgttctcat cgaggtcttt ggtggcttct tcagaaacgt tggggatatc cggcgtcagt
     3181 tcttcttcgc cgagtttggt atcacgtact tccagttcat attcagaaat gtgaacggaa
     3241 gtaaacatat cttcttttac gactctttcg ctgattacaa tcgcatcctc gaagttgtat
     3301 cctttccagg gcatgaaggc cactttcagg tttttaccaa gtgccagttc cccgttctgg
     3361 gtggcatatc cttccgtcag gaagtcgcct tttttcaccc gctgaccttt cttcaccgca
     3421 ggggaaagat tgatacaggt ttcctggttg gtcttgataa acttcgtaag acgatagatc
     3481 cgcaggtcat cttcaaaact cacgagtttt tcagaagcat ccctttcata acggacgtgg
     3541 atttcattgg catccacaaa ttcaaccacc ccttctccgt cagagtggat ctggatcctt
     3601 gcatcacggg ctgcttttcc ttcaagaccg gtacctacaa taggtacatc cggacgaagc
     3661 aggggcactg cctgacgttg catgtttgat cccatcagcg cacggttggc atcatcatgt
     3721 tccaggaatg gaatcaggga ggcactcaaa cccacaatct ggttgggggc cacgtccata
     3781 tattccactt cattcttgtc gaggatcggg aagtccccgg tttcacgact ttgaattctt
     3841 tcatctttaa aatttccttt ttcatccagc tgggtattgg cctgggcgat ttttgccaga
     3901 tcctcttcct cagcactcag gaaattcagt tctttcatgt tcactttacc ttcatgcact
     3961 ttgcggtatg gggtttcaat aaagcccata tcattgatct tcgcatgtac gcaaagggtg
     4021 gagatcagac cgatgtttgg tccctccggt gtttcaatgg tacagagacg cccgtagtgg
     4081 gaatagtgta cgtcacgtac ctcgaaaccg gcgcgctccc ggctcagacc accgggcccg
     4141 agtgcggaaa tacgacgttt gtgggtgatc tcggaaagcg ggttggtctg atcgaggaac
     4201 tgggacagct gtgatgttcc aaagaaggag ttgatcacag aagaaagggt ccgggcattg
     4261 atcaggtcaa ccggggtaaa tacttcatta tcgcggacgt tcatacgctc acggatggta
     4321 cgggccatac gggccagacc tacaccgaac tgagcatata attgttcacc tacagtcctt
     4381 acacgacggt tactcaggtg atcgatatca tcgatctctg ctttaccgtt ggtcagacgt
     4441 acgaggtatt taatgatgag gataatatca tctttagtca gtgttttctt ttccagcggc
     4501 aggtctacat tcagtttgcg gttgatcttg taacgaccta cttcgccgag gtcataacgt
     4561 ttgtcgctga agaataattt gtcgatgata ccccgggcgg tctcattatc gggtgcatcc
     4621 gcgccacgga gctgacggta gataaattgt accgcttcca gctcactgtt ggaagtatcc
     4681 ttgttcaggg tattgtagat gatagcatag tcgccaccca catcttcgcg ctgaacaaac
     4741 acactggcca cttccatttc ggaaatcgtc tcaatggctt cttcatccag cacggtatcc
     4801 ctttccatta cgatctcatt ccgctcaatg gaaaccactt caccggtgtc ttcatccaca
     4861 aaatcctcta cccaggtacg gagtacacgg gcagcaaggc gtttgccgac atatttgctt
     4921 aaggttttct tttccgcttt tatttcatcg gccattccga atagtgtcag aatgtccttg
     4981 tctgtttcaa atccaattga acgaagcagg gtggtaaccg gaaatttctt cttccggtca
     5041 atgtaggcat acatcacgtt gttgatgtcg gtggcaaact ccatccaggc tcctttgaaa
     5101 ggaatcacac gggcggagta gatcttggtg ccattcgggt gaacggactg accaaagaaa
     5161 acacccggtg aacggtgaag ctgtgaaacc actacacgct cggccccgtt gatcacgaag
     5221 gtaccgcggg gagtcatgta gggaatattg cccaggaaca cgtcctgcac gatggtctgg
     5281 aaatccacgt gctcttcatc gttacagctc agacgcagtt tcgctttcag cggcaccgca
     5341 taggtcagac ctctttccat acactcttcg atagtgtacc ggggcggatc gatgaagtaa
     5401 tccaggaatt ccaggacgaa aatattccgg gtgtccgtga tcgggaagtt atccttgaat
     5461 acacggaaca aaccttcgat atttcgctta tccggggttg tttctaactg gaaaaattct
     5521 ttaaacgatt gaatctgtac ttcaagcaga tcgggtgttt cagccaggtg cttgatttta
     5581 ccgaaatcca ctcttgagtt cacttttgtt gaagacatat gcgtaaaacc ggtttttaca
     5641 acgttgagga cacaccaatc atgttcatgc tctcacttta gaaaaaatga aagtgcatga
     5701 aattcagcta cttacacatg gattgggatg tcaaaatata tttggccaaa ataaaggatt
     5761 gcaaaggtag ggattatatg ttctcaacca agaaaagttt gctcacaata acccggccaa
     5821 aaaaccggta aatcaggggg ttttacccct tccaaaaacc ccgtttctgg ttaaaattgg
     5881 gccatgaaaa tgaaggaaaa actggcgggt tacctgaccc tgagccggaa ggaaagaacc
     5941 ggggttatac tactcgggct gcttattatt tccctttttt tactcccccc gctccttccg
     6001 gtaccgggcc ctcccccggt cagtccggcc gataccgcgt ggatcgtggc gatgaaaaaa
     6061 ctggaacaaa aagaagaatc ttatccacag ggcgtcccat cgtcccgtga agacccggtc
     6121 cgccccccag cccgagccgg gtacgggtat ccggcaagat ccggaacacc ctcactcttc
     6181 aacttcgacc ccaatacgtt gagagaagag gggtggcagc aactggggtt gcgggagaag
     6241 acgattaaga cgattttaaa ctacttgtcc aaaggaggga aattccgtaa gccggaggac
     6301 ctgcaacggg tttatggcct gtttccggac gaatacgagc gtttgagacc ctatatccgt
     6361 attggggaag taacctcttc cccattaccg gcatggacca atgaaaaacg ggattttgaa
     6421 aagcccgcta aggtcactgc tgatacaaga cgggtgatcg ttgaaattaa tacggcggat
     6481 accagtgcgt tgattgccct tccgggaatt gggtcaaaac tcgcccaacg aatcatcagt
     6541 ttccgggaaa agctgggagg attttattcg gtgaaacagg tggcggagac ttttggattg
     6601 gctgattcgg tataccggaa gatcgaaccc ttattaaagc tggaagtgaa aacatggcgg
     6661 aagatcaata tcaataccgc tacagccgaa gagttgaagg ttcatccata cctgaaatgg
     6721 aatgtgatca atgccttact ggcctaccgg aaggaacatg gttcattccg gaatacagat
     6781 gagctgaaac aggtcatggc ggtgacggat gaaatatacg ggcggatcgt accttacctg
     6841 gttacagaat aaaaaattat actgaactgc tacaagctaa aatttagttc aacccctttg
     6901 gggttgttag gatctctaca caactttacc ccgggttgca cccggggcta ttaaaagttt
     6961 aagccctgcg ggctatacat tcatttccaa aacccatacc gctgaaaaaa aagtatggat
     7021 aaccatagaa gtaatgttcc tcttgaagga attacagaac ctctttgaag ctggaaaata
     7081 gtattcagaa aagtgaaaaa atcccatatg gttttgtttg catcaatcag actgatgcac
     7141 agttaaaagt caggttactg ataaccccgg aggggttgaa caattcatag ccccgggtgc
     7201 aacccggggt tagttgaagg tatgattctc ccaaccccaa cggggttgaa ttaagctggc
     7261 tggcagtgaa actgctggcc atccgataag ccgaaatccc ggataaataa aaaagggtgc
     7321 cgtatcgaca cccctttttt atcagtcagt tgaaattaag caatttcaac atcagcacct
     7381 gcttctttca gtttagcggc gatttcttca gcttctgctt tagaaacacc ttcttttaca
     7441 ttcttcggcg caccgtcaac caggtctttt gcttctttca gaccgagacc agtcagatct
     7501 ttaacgatct taaccacgtt cagtttggaa gcaccaccac tcttcagtac tacgttgaat
     7561 gctgtttttt cttcagcagc agcagcacca ccaccatcgc cgctggctac aactactgca
     7621 gcagctgcgg gttcgatacc gtactctgtt ttcaggaaat cagctaattc ctgtacttct
     7681 tttactgtta agcctaccag gctttctgct aatgctttta cgtctgccat tttgtttgaa
     7741 tttttaccgt cttcatttcc ataatgggat ccgacggcgg tttttaaaaa aattgtttat
     7801 atagtaaagt ggcggtagaa ccgggtggtc cgggctcagg ccctcgccgc tcgcgactca
     7861 ttgctcgccc ctgtatactc cttactctgc agcagcagcc acttccgcgc cgcctgcttt
     7921 ctgctcgtgg tggtgcagta acgcagccag tacacgtttg gcaggtgatt gcagcaaccc
     7981 gataacctca ccaatgagtt cattcttggt tttgatctgg gtcagtgctt tcaggttgtt
     8041 atcgcccatg tatacatcac cgttgatgaa agccgctttc agaacgggtt tatcaccgtt
     8101 gctttcttta cggaaagaac tgatgatcag cgccggttcc ttggggttct cagagaacat
     8161 cagggccgtc actttgtgca gtgaatcata catacttgcg tatttctcac tgtccagtga
     8221 ttccagcgcc tttttaatca gggtgttttt ggccactttc atttccacct gtttgctgaa
     8281 acaggcacgg cggagtttac ccacctgcgc tactgaaaga gattcagtat cggcaatata
     8341 gaagttgttg tattgagcaa acttgccttt cagcagttca atcacttcgt ttttttgatc
     8401 tttagtcatt ttacttaagt tttttatcgg tcaggccgtt tggccccgtt aaaaattacc
     8461 tggccggatt aaacggccag accggtttag tgaacaaatg ctttggtgtc aatagcaata
     8521 ccaggactca tcgtgctggc catgctgata cctctcaggt atgtaccttt tgcagaagag
     8581 ggtttcgcct tgatgattgc attcagcagc tccaggctgt tttcagcaat tttctcagga
     8641 gtgaaactta cccgtccgat agaggcatgg atgataccta ctttatccac tttgaaagcg
     8701 atcttaccgc cttttacatc attgatagcg tttgccacat cattggtaac tgtaccggtc
     8761 ttggggttcg gcatcaggtt acgggggccc agtactttac cgagtttacc aatcttaggc
     8821 ataacagaag gagtagcgat gattacatcc acatctaccc atccgccttc aattttggta
     8881 acatactcgt ccagtccgac ataatccgca ccggctgctt ttgcttcggc ttctttatcc
     8941 gggggacaaa gaacgagtac ttttttagtt ttaccagtac catggggtaa acttaccgta
     9001 ccacggattg cctggtcagc ttttttagga tccacaccca gacggatgtg caggtccacg
     9061 gaagcatcga atttgcaggt ggaaattgtt ttcaccagcg tggaagcttc ttttaaggaa
     9121 tatgctttgt ttgcgtcaac ttttgtatcg gcaacttttc tttttttact aatgaatgcc
     9181 attttaattt cttttaagga tttcagaaag tccccttcag ggggttaagg gattaattct
     9241 cccagggagc tttaccttcc acgttgaggc ccatgctgcg ggcggtaccg gctaccattt
     9301 tcatggcgct ttcgatagta aaacagttga ggtcaggcat cttgtcttct gcaattgcct
     9361 ttacctgttc ccagtttact ttacccactt taacacggtt gctttctttg gaaccctttt
     9421 gaatttttgc ggcttccatc agctgaatag ctgcgggagg cgttttgatt acaaagtcaa
     9481 aactcttgtc tgtgtagacg gtgattaata cgggaagtac ttttcccatt ttgtcctgtg
     9541 ttctagcatt gaactgctta cagaattcca tgatgttgat acccttggaa cccagcgcag
     9601 gaccgatcgg aggtgcaggg ttggcctggc cacccttaca ctgcagcttt acaaagccgg
     9661 agatttcttt tgccattttc tttaatttat tggttctagc gacctttcga tttgtctact
     9721 tggtctccca aattccttgt tgtcccattt ccgggatttc tctaaagaac taattggggc
     9781 tgcaaaagta agcggaaaag ctttactggc aaaggttttc accctttttt tcgatcaaca
     9841 atgccaaaaa aaagccatcc gtgtgaggcg gatggctttt actattttat agtaccggtt
     9901 aactgatctt ctctacctgc atgtagttga gttccacagg agtagaacga ccgaaaatct
     9961 tcaccgtcac cttcagtttc tttttctcat cattcacttc ctcgatcacc ccgttgaaat
    10021 cgttgaatgg cccttcaatg atcttgatgg tttcgcctac gataaacggc tcactcatgc
    10081 tcataccgcc tacttctgcc atttcatcca ttttacccag catcttgttt acttcggctt
    10141 tacggagggc aatgggatct tctttcccca ggaaatggat gacattggtc gtattcttga
    10201 taatgtctct caggtcgtcg tttaattttc catccgctat ctcaatcatc acataaccgg
    10261 gataatagtt acgttcccgc attacttttt taccactggc tactttgtac accttttcaa
    10321 ccggcaggaa aacctgcttc acgatctcac tccagccacc acgggagatt tccttatcca
    10381 ggtattcctt caccttcttt tccttgccgc tcaccacacg cagtacgtac catttggttt
    10441 cggcctgctt gacattctcg gtattcggtg ttgtttccat tgaaaaaagt ttgaatataa
    10501 attagatcag gctttgtaaa gttggttata gacaaactta agcccgttac cgatggcaga
    10561 atccattgcc agtacaatga aagttataac aatggtggca gccagtacaa tcatggtaga
    10621 ttgctgtaat tgctgccagg tcggccagga tactttctcc gtcagctcct taaagctctc
    10681 tttgaagtag gttgtgattt tattcataat acgctgtttg cacgggcaga gagattcgaa
    10741 ctcccatcaa cggttttgga gaccgctatt ctgccattga actatgcccg tttttaatac
    10801 tttattttaa gaactcccat caatcccgga ggtccgggac tattctgcca ttgaatgctg
    10861 acgaagctgc gcttgtccgc atcccgacag gctttgcctc gtcgggacta tgcccgtaag
    10921 aatcaaaagt caccggtcgt tcggaaaatc cggcagacca atgacttttg aagtatcaaa
    10981 tataaaagat tatttaataa tctcagtaac ctgtcctgca cctactgtac gaccaccttc
    11041 gcggatcgcg aatttcagac ccttttccat cgcgatgggc tgaatcagtt ttacattcag
    11101 tgaagtgtta tcaccaggca taaccatttc agttccagcg ttcagttcaa cttctccggt
    11161 tacgtcagta gtacggaaat agaactgggg acgatatttc tggaagaacg gagtatgacg
    11221 tccaccttct tctttgctca gtacgtaaac ctcagctttg aaatcagtgt ggggagtgat
    11281 agaaccgggt tttacgatta ccataccacg acggatctgg gttttctcaa taccacggag
    11341 cagcagacct gcgttatcac cggcttcacc ttcatccagc agtttgcgga acatttccac
    11401 acctgtacag gtagaagtca ggggctcagc gataagacca acgatctcaa caggctcacc
    11461 cactttaata cgacctcttt cgatacgacc tgtggcaaca gtaccacgac ctgtgataga
    11521 gaatacatct tccacagaca tcaggaacgg ttggtcaacc gggcggggag gcaggggaat
    11581 atattcgtca actgcagtca tcagttcttc aacctgctta actgcttcag catcaccttc
    11641 cagggctttg atagctgaac ctttgatgat cggtgtgtta gcaccatcaa aaccgtagaa
    11701 agtcaggagg tcacggattt ccatttccac cagttccagc agttcggggt catcaaccag
    11761 gtcaacttta ttcatgaata ccacaatctt aggtacaccc acctggcggg ccagcaggat
    11821 gtgctcttta gtttggggca taggtccgtc agtagcggca accaccagga tcgcaccatc
    11881 catctgggca gcaccggtga tcatgttttt aacatagtca gcgtgacccg gacagtcaac
    11941 gtgtgcgtag tgacggttag ccgtctggta ctcaacgtga gctgtattga tggtgatacc
    12001 cctttctttc tcttcaggag ccgcatcaat ttcatcatat ttttttgcag tcgccatacc
    12061 cttagaagct aagatggtgg taatggctgc ggtcaaagtt gttttaccat ggtcaatgtg
    12121 tccgatggta ccaacgttaa cgtggggttt gtccctctta aaggtctctt ttgacattgt
    12181 tatcggtttt aaagttgtgt tttacaatta ttgttttttt atcaggaagc cttcagtatt
    12241 gttaccaccg gccatctttc aacacattgt ttttcaattt tttaactgcc tcaaaaaaac
    12301 tgagccgtta tggggaattg aaccccagac ctcttcctta ccaaggaagt gctctaccac
    12361 tgagctacaa cggcttcttt acacgtttag ctggcagacc tcttctcccg tacctacggg
    12421 atgctccacc actgagctac aacggcttct tacagccggc agccagcagt acacagtatt
    12481 cagccagctg aggctgactg ccaactgcca actgccaact gaaggctgat tactgacaag
    12541 agcgggagac cgggctcgaa ccggccacct gaagcttgga aggctaccgc tctaccaaat
    12601 gagctactcc cgcttttacc caccgaagct ttagcgaagg tgggtttgtt tctaagaact
    12661 ttttatgatg tcctacttca ccgaagctag gtaggacgct tccctctttc gctaaagcta
    12721 tagagggcac tgtgggcaag gatggattcg aaccaccgaa ctccgaagag gacagattta
    12781 cagt
//
LOCUS       DAOPMW010000058        12665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5141_length_12665_cov_10.296431, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000058 DAOPMW010000000
VERSION     DAOPMW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12665
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_12665_cov_10.296431"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..1184
                     /locus_tag="PLU11_04840"
     CDS             435..1184
                     /locus_tag="PLU11_04840"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPN58471.1"
                     /translation="MKRVIPPVLLVALALPFSAPLLAQNNPKYEFGINLGFTVYQGDL
                     TPQQLGSFRTQKLALGLQAAKLLSPSFSVRAAFLRGRLKGDDGLYANPEYRQQRNFNF
                     STPVTEFTGHFVWNPMARNYAVKGFSPYFFAGAGFAFLRIKRDWSGLNSSYFDSETST
                     IFSGLAADTVHSVPRLIPVIPVGMGIKYFITPKLGMNAEAAYRLGYTDYLDGFSEAAN
                     PAKKDHYLNYSIGIIYRSGTKNRLSCPKISY"
     gene            1507..2226
                     /locus_tag="PLU11_04845"
     CDS             1507..2226
                     /locus_tag="PLU11_04845"
                     /inference="COORDINATES: protein motif:HMM:NF037012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58472.1"
                     /translation="MKPSIAKIYLSGQRDVESSPGFRCHHLFNGDTRFHAYKTALGDL
                     CRFDDILLAAGKSWPRDAGTSAYVVLIPLSGSIEYVIGNSAPGVISAGQLQVFSIHEN
                     ETYRVSNLSPDKGVNYLELFLVKKPEMPDMVPAPADADLNKFMNVLLQVAAVGSGSGD
                     QWRLSIGKFSGRGETTYQRKYPGKGLFMFVIDGLFEVEGRLLHARDGLALWDTAEAGV
                     EARSNEALLLVVESNFSAAVP"
     gene            complement(2207..4288)
                     /locus_tag="PLU11_04850"
     CDS             complement(2207..4288)
                     /locus_tag="PLU11_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58473.1"
                     /translation="MYRFSLLSIWLFFAVSGIAQEEINDSTKSLDEVIIRAFEQNRKA
                     RFSTASVKIIDLKNADRNNKLSLVNGLNTVPGVRMEERSPGSYRLNIRGSSLRSPFGV
                     RNVKVYWNEIPVTDPGGNTYFNQFAYTNFSTIEIYKGPAGSMYGAGTGGLLLMNNFQR
                     WKPGLSLEYLKGSYSTDALFATVRYGSDENRHQISYAHNQSDGYRSQSAMRRDNISWQ
                     SQLKLSGKQELTASLLYTDLYYQTPGALTLSEFTSNPKAARPAGGGFPSAVNAKAAIF
                     QQTMTAGFTNAYQFNPRFKNTTTLYGAFAQIRNSAIRNYERRNEPGYGGRTVFVYNKE
                     TVTGQRKNSLQLVAGSEFQQGRFNIQVAKNRNGNPDTLQTNDDISYTALSVFVQGDLQ
                     LNQQWLFTAGISLNKATASLTRLSHYPILTQKRNYRNELAPRFSLKKFITEDFSLKST
                     VSRGFSPPTVSELLPSTGVISTALEAEYGWNYEITANHDFFQHRLQTELTGYYFRLNE
                     ALVQRRDLSGADYFVNAGDVRQQGVELQADYTQTFKKGLIDYFLIQAGYSYIHYRYGQ
                     FIKGTDDFSGKTVPSVPANTLSVLFDLHTRTGFYFNSTFYNASRIYLNDANTASADPY
                     QLLGARVGWKKTGKKKYTMNFYAGADNLLDQQYSLGNDINAAAGRFYNAAPRRNYYAG
                     ISLGRIRAPQH"
     gene            4343..5674
                     /locus_tag="PLU11_04855"
     CDS             4343..5674
                     /locus_tag="PLU11_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN58474.1"
                     /translation="MTAKNKLNLTDISLIVVSLVIGMGIFRNPASVAATSGTSTIFFL
                     LWIAGGIIALFGALTYAEIGQRLPAMGGYYKVFAECYHPSVGFSVNSIILISNAASLA
                     VVALIGADYVSDLLYGKPSGVLFNSIVAITAVAAFFVVNLFGLKTSSRTQNVLIIIKV
                     GLMVLLISALFKGVVIEPHGYEEGSPLFTLNDKSAFSLFVISLIPVCFAYGGYQQTIN
                     FGGEVKETRIIPKGIIIGIIIVIGLYLLINVAYVEVIGYEKMKNASAIGALLCEAWFG
                     KAGGKVFDALMFLSVLAYVNILLMSNPRVMFAMSQDKVLPKIFSYRHPKTEALVPGLL
                     TFSLITIIVTFFGKGVDNILNFTMFLDSIGMSTSAATLFILRKRKQNEAMITGNWNRF
                     TPMLAGFFVFSYFMIAVGVVIKDVNAALIGTGLLAFFLAVYYFFYHRKKPD"
     gene            5871..7319
                     /locus_tag="PLU11_04860"
     CDS             5871..7319
                     /locus_tag="PLU11_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN58475.1"
                     /translation="MRRSIQLALMLLFCSAAGAQEKWDLRQCVDHAVKNNISVRQTDL
                     QSRFSALTYSQSKSAQLPSFNYSGSVGYRLGRSENPTTGVLEDNNFLNLGMQLQSGVT
                     VFNWFARKNTIESNRLSYEADKQQTEKIKNDISLNVAVAYLQILLAREQANIAAAQVE
                     LSLSQLENTRKRVEAGSLPELNAAELEAQLARDSSSLITAQANAVQFLLQMKSLLNLD
                     AGQPFDIATPPVNLIPVDPIADLLPEAVYASAIKFMPQQKINELRILAAKKNVDVAKA
                     GLYPSFTAFGSLSTNAINFRRPVYNQVFNGYVPTGARVDAGGGTFYPVEVPDYSDGTT
                     VVRYFKPGSITKQFNTNFGQSVGIGISVPILNGKAAHTAWDRSKVSLQLQELQKEQGD
                     LQLKQDIYKAYNDATAALQKYNADVKAMQTAEKAYSFASKRYELNLLSAYEMVTSQTN
                     MQRARIQALYSQYDYVFKMKLLEFYKGQGLKL"
     gene            7330..8685
                     /locus_tag="PLU11_04865"
     CDS             7330..8685
                     /locus_tag="PLU11_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN58476.1"
                     /translation="MNKKLKWVLIILGILALIFIIMKVAGGGDKTVKVATEKAVKRTI
                     VETVNASGKIYPEVEVKISPDISGEITELNVEEGDSVRKGELLARIYADIYALQRDEA
                     ASRVNQSAATVDNSQAAIESLKANLELAQQSYNRNKKLFDEKVISKSELEQFETTLRS
                     AQANFNAAQQNIKSLQAGVQSTQVGLSKANKDLSRTSLVAPMDGVISSLKIKKGERVA
                     GNSFNVGTEMMTVADMSVLEVRVDVGENDIVKINIGDSADVEVDAYNNRKFKGVVTKI
                     ASSTKAGSLTSSNDVTNYEVRIRLDKDSYKDLSSRTFPFRPGMNASADIKTRRVENVL
                     AVPIAAVSARVKGSDQSMADKKKENEASKDENEAQNQNGIVVESDELEEVAFVLLPDG
                     IVQKRVVKTGVQDISYIEILSGLKEGEELITAPYAAVSKDLKDGMKVTVVPKDKLFDK
                     K"
     gene            8689..9726
                     /locus_tag="PLU11_04870"
     CDS             8689..9726
                     /locus_tag="PLU11_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPN58477.1"
                     /translation="MRFGVIGSGSWATALAKIITDKKQPVNWWVRNAETIAFFMKKSH
                     NPHYLSSARFDTSLLHLSTDVREVVRNSDVLVIAIPSAYAASSLAGLATEDWKGKKVI
                     SAIKGLLPEKNILLNYYMQQEFGLGLEDYFAVLGPCHAEEVAEEKLSYLTFSGIDTTT
                     AARMAEHFRTDYINTVVNHDILGVQYAAVLKNIYALGAGIAHGLDYGDNFLSVYIANA
                     ADEMAVFLRQFGAEHITVGEHEGEDPLTHRKTPNYAASVYLGDLLVTCYSLHSRNRRF
                     GNMIGRGYTVKSAELEMNMVAEGYNAARCIHLINQSLQAPIPIAETIYKILWEEVKAA
                     EGFKNIEEVLV"
     gene            9906..10112
                     /locus_tag="PLU11_04875"
     CDS             9906..10112
                     /locus_tag="PLU11_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58478.1"
                     /translation="MIAFFQVLFFIASLVLLVMNFLDLNKWNESRKSGAVKAYPIRWF
                     TFILTSLVALGCFLNILVSWISKA"
     gene            complement(10109..11218)
                     /gene="hemW"
                     /locus_tag="PLU11_04880"
     CDS             complement(10109..11218)
                     /gene="hemW"
                     /locus_tag="PLU11_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPN58479.1"
                     /translation="MAGIYIHIPFCKSACHYCNFHFSTSLHYKNELLAALLKESRLQQ
                     SFPGMESVSSLYFGGGTPSLLENEEVNQLIVEIRTHYNLLPDAEITLEANPDDLTEQK
                     LQAWKSAGINRLSIGVQSFFEEDLRWMNRAHTSSQATEGLELALRYFDNITIDLIYGT
                     PGLTDEKWQQNVAKAIALGIPHLSCYALTVEPKTPLDKLIRNEQYENVDPDQQSSQFL
                     QLMHWLEEAGYEHYEISNFAVPGKRSRHNSSYWKQQPYLGLGPSAHSFYGNERQWNIA
                     NNNTYIKALQEDCIPFEKEILTPAQQANEYIMTSLRTMEGLHLQRMNEAQQKQLEKGA
                     AKYLNSGLLLFREDHLILTREGKLLADGIAADLFF"
     gene            11281..>12665
                     /locus_tag="PLU11_04885"
     CDS             11281..>12665
                     /locus_tag="PLU11_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58480.1"
                     /translation="MRKYRLQISVLLALLTAICTQGQEKYTLRVRYADSLSALMADTS
                     LVQQTFSTRIDCSNYLAQLPARLQARGFVTASLDSIRFDSTYAAIVLFLGQRYQWARL
                     DTRLVEPGLLEAVGWRDRNFADKPMDFVQVRNWQEKFMDYLENHGYPFAKIFLDSLQM
                     DQDKVSGILKVDKGPLYKIDSIRVYGNAKITSAYLQRYLAIPNGSRYEREKLMRINQK
                     IRELAYVEEEKPFDITLLGTGSVVNLYLKQKKSSQVNALIGFLPNNDQLSSKKLLLTG
                     EANILLRNSLGAGETIGLNWQQLQVKSPRLNIIYQHPYLFQSAVGLDFAFDMFRKDSS
                     FVNVNLQLGAQYVLNKNQSGKLFIQRFQTIVSEGGIDSSYVVTYRRLPDLGDVSSFNV
                     GIDYELNNTDYRLNPRKGNELRIIGSVGTKTLKKNNSVLEYKDPADPLFDFGTLYDTV
                     KLKTYQFRLRLT"
BASE COUNT         3266 a         2833 c         3153 g         3413 t
ORIGIN      
        1 gacttacacc ggatttccct tttaatctcc cgcatgaagc gggggaacca aatcagtgca
       61 aatgtaagat gcctgtccaa atctgaagta agagttatca acaacgtgga ttactgattt
      121 tttctctcgt ttttcgggta aaagcattga ttgaacgaaa tggatgatgt ggtagctccc
      181 ttcagctacc ctttaggttc gggaaacctt caaccgaaaa caggcttccc ggtctgattg
      241 aatattcaga aaagtttaat cggactttgt cattttttat acattttttt ttgaagcatt
      301 tctttaacaa ttctgcgata atggaaagcg catttctgcg tttgactgct aacatctgct
      361 tatgtttttt caaagttggg gattaagcga tgtcgcttct gcccggattt ttcactttta
      421 taaatcattg tccaatgaag cgtgtaatac ctccggtttt attagttgct ttagcacttc
      481 ctttcagtgc accattgtta gctcaaaaca atccgaagta tgaatttggg atcaacctgg
      541 gctttaccgt gtaccagggt gatctgactc ctcaacaact gggttctttc aggacgcaga
      601 aattagccct tggtttacag gcggctaaac tgctcagtcc ctctttttcc gtcagagcag
      661 catttctccg ggggcgactc aagggagatg atggtcttta tgccaacccc gaataccggc
      721 aacaacggaa ttttaatttc agcaccccgg taacagagtt caccggccat ttcgtttgga
      781 acccaatggc caggaattat gcggtgaaag ggttctctcc atactttttt gccggtgccg
      841 gttttgcttt tctaaggata aaaagggact ggagcgggct caacagcagt tattttgatt
      901 cagaaacatc caccattttc agcggactgg ctgcagatac tgttcattca gttcccaggt
      961 tgatcccggt aatccctgtg gggatgggca tcaaatactt cattactcct aaactgggta
     1021 tgaatgctga agccgcctac cggctcggat atacggatta tctggatggg ttcagtgaag
     1081 cagctaatcc ggccaaaaag gaccattacc tgaattattc aattggtatt atatacaggt
     1141 ccgggacaaa gaacaggctc agttgcccga agatcagcta ttagcaaatg aatagaccgg
     1201 gaccttgttt caacagcgac gtcttttaat aaaaggaatg actttattta accattgtgg
     1261 gaaatgaatt tacccaaata ctcattacag cctcgtgata aaatccaaca ttcatccgga
     1321 gaagatgctc ctgtacccgg catcttctcc atactgctga agctgccgta aacctgaaat
     1381 gtatgataca gtccaggcag aactttatgt tttttttgga taaatagcgg aattccggca
     1441 tcaaattggt gtcagggatg gtcacgctga cccataataa taagcattca tcacaaattg
     1501 actttcatga aaccatccat tgctaaaata tatttgtccg ggcaacggga cgtggaaagc
     1561 agcccgggtt ttcgttgtca tcatctgttt aatggggata cccgctttca tgcgtataaa
     1621 accgcactgg gtgatctctg ccggtttgat gatatattgc tggctgccgg aaaaagctgg
     1681 ccgcgggatg ccgggacatc agcttatgtg gttttaattc cactgtctgg ttcgattgaa
     1741 tatgttattg gcaattcggc acccggggtt atttcagccg gacaacttca ggtcttttct
     1801 attcatgaaa atgagactta ccgggtcagt aacttatctc cggataaagg ggtgaattac
     1861 cttgaactct ttctggttaa aaaaccggaa atgccggata tggtccccgc acccgctgat
     1921 gcagatctga ataaattcat gaatgtactg ttgcaggtag cggctgttgg cagcgggagc
     1981 ggggaccaat ggaggctttc catcgggaag ttcagtggaa gaggtgaaac cacttaccaa
     2041 cgcaaatacc cgggcaaagg cttattcatg tttgtgattg acggcctatt tgaagtggag
     2101 ggtcgtttat tgcatgcacg tgatggcctg gccctttggg atacggctga agcgggggtg
     2161 gaagctcgta gtaatgaggc gctgttgctg gtcgttgaaa gtaattttag tgctgcggtg
     2221 ccctgatccg gccaaggctg atgcctgcat aataattacg ccggggagcg gcattataaa
     2281 accgcccggc cgctgcattg atatcattcc ccaggctata ctgttgatcc agcaggttat
     2341 cagcacccgc atagaagttc atcgtatatt tctttttccc cgtttttttc cagcccaccc
     2401 gggcacccag caactggtaa ggatcggctg acgctgtatt ggcatcattc aggtaaatac
     2461 gtgaggcgtt gtaaaaagtg ctgttgaaat agaaaccggt ccgggtgtgc agatcaaaaa
     2521 gcactgagag ggtattggct ggtaccgagg gaacggtttt cccggaaaag tcatccgtgc
     2581 ctttaatgaa ttgaccataa cggtaatgga tatacgaata tcctgcctgg attaaaaaat
     2641 aatcaatcaa tcctttttta aaggtctgtg tatagtccgc ctgcaattca actccctgct
     2701 gacgtacatc cccggcattg acaaaataat cagcccccga aaggtcccgg cgttgaacca
     2761 gtgcctcatt taacctgaaa taataaccgg taagttcggt ctgcaaccgg tgttggaaaa
     2821 aatcatgatt cgcggtgatt tcatagttcc aaccgtattc agcttccagg gctgtactga
     2881 tcactcctgt ggagggcagc aactcggata ccgttggtgg tgaaaaccca cgggagacgg
     2941 ttgatttcag gctgaaatct tccgtaataa attttttgag ggaaaagcgc ggggccagtt
     3001 cgttccggta atttcttttc tgcgtgagta tcgggtaatg gctgagacgg gtaagactgg
     3061 ctgttgcttt attcagactg ataccggcgg tgaaaagcca ttgctgattc agttgcaggt
     3121 cgccctgaac gaaaacactg agtgcggtat agctgatatc gtcattggtc tgtaaagtat
     3181 ccggattccc gtttcggttt ttggcaacct ggatattgaa tcgtccttgc tggaactcac
     3241 tgccggccac taattgcagg ctgttctttc gctggccggt aaccgtttcc ttgttataca
     3301 caaaaaccgt acgtccgcca taacccggtt catttcttct ttcataatta cggatggccg
     3361 aattacggat ctgagcaaaa gcgccataca gcgtggtggt atttttaaaa cggggattga
     3421 attgataagc atttgtaaat ccagcggtca ttgtttgctg gaagatggcg gctttggcat
     3481 ttacggcgct gggaaaacca ccaccagccg gcctggccgc tttggggttg ctggtaaatt
     3541 cggacagcgt cagggcaccc ggtgtctggt aatacaggtc ggtatataag agtgaagctg
     3601 taagttcctg tttaccggaa agttttaatt gggactgcca gctgatgtta tcccgtcgca
     3661 tagctgattg actgcgatag ccatcactct ggttgtgggc ataactgatc tgatggcggt
     3721 tttcatcgct gccgtaacgt accgttgcaa atagcgcatc ggtgctatac gaacctttca
     3781 ggtattccag gctcaggccg ggtttccagc gttgaaaatt attcatcagc agcagtccgc
     3841 cggtgccggc tccgtacata ctgccggccg gacctttata aatttcaatg gtggagaaat
     3901 tagtataggc aaactgattg aaatatgtat tcccacctgg atccgtaacc gggatttcat
     3961 tccaatacac tttcacatta cgcaccccaa atggagaacg gagcgagctg cctctgatat
     4021 taagccggta actgcccgga cttctttctt ccatccgtac accgggtacg gtgttcagtc
     4081 cgttgacaag tgacagctta ttgttgcggt ccgcattttt caggtcaatg atttttactg
     4141 aggctgtact gaaccgggct ttccggtttt gctcaaaggc ccggatgata acttcatcga
     4201 gcgattttgt actgtcattt atttcttcct gggcaattcc ggaaacagcg aaaaacaacc
     4261 aaatagatag aagcgaaaat ctgtacatca gcgtcggttc aggcggttaa agttagtatg
     4321 ttttagttgt atctttccgc ttatgacggc aaagaacaaa ctcaacttaa cggatatcag
     4381 cctgatcgta gtaagcctgg tgatcggtat gggtattttc aggaatccgg cttccgtagc
     4441 agcgacttcg ggtacaagca cgattttttt cctgctctgg attgccggtg gtatcattgc
     4501 gctgttcggg gcattgacct acgcggagat tggacagcgg ctgccggcta tgggcggtta
     4561 ttataaagtc tttgccgaat gctatcatcc atccgtcggc ttttcggtca actccatcat
     4621 cctgatcagt aatgcagctt ccctggccgt agtagccctg atcggtgccg attatgtgag
     4681 cgatctttta tatggaaaac cgagtggtgt tttgtttaat tcaatagtgg ccatcacagc
     4741 tgtagctgct ttttttgtgg tcaacctgtt tgggctgaag accagcagcc gtacccagaa
     4801 tgtactcatt attattaaag taggattgat ggttttactg atctccgcct tgtttaaagg
     4861 ggtggtgatt gaaccacatg gttatgaaga aggttcaccg ctattcacac tgaatgataa
     4921 atccgccttt tctctgtttg tgatatcact gatccctgtg tgttttgcgt atggggggta
     4981 ccagcaaacc atcaattttg gcggagaggt caaagaaacc cggatcattc ccaaagggat
     5041 catcatcggg attatcattg taatcggttt atatctgctc atcaatgtgg cgtatgtgga
     5101 agtgatcggg tacgaaaaaa tgaaaaacgc atccgctatc ggggccctgt tatgtgaagc
     5161 ctggtttggt aaagccgggg gtaaagtatt cgacgccctg atgtttcttt ccgtactggc
     5221 ctatgtcaat attctcctga tgagtaatcc acgtgtgatg tttgccatga gccaggataa
     5281 agtattacca aaaatatttt cttaccgtca tccgaaaaca gaagcgctgg tgcccggcct
     5341 gctcactttt tccctgatta cgatcattgt tacttttttc ggaaaagggg tcgataatat
     5401 tctcaacttc accatgtttc tcgacagtat cgggatgagt acatctgccg ccacattatt
     5461 tattttacgg aagcgaaagc agaatgaagc catgattacg ggcaactgga accggttcac
     5521 cccgatgctg gccggatttt ttgtcttcag ctattttatg attgccgtgg gggtcgtgat
     5581 caaggatgtg aatgcggccc tgatcggtac cggtttactg gccttcttcc tggccgtata
     5641 ttacttcttt taccaccgga aaaagcctga ttaatttttc tccagctgct gatcagccgg
     5701 gtaagtgaat aaccgctaaa aggcgggtgc ccggattccc tctttttagc gcagaaaagg
     5761 ggtgggaatc ccctgctgaa atcaggcccg gaaccccctg ttcccggatt ttcctgccgc
     5821 ttgtcaaaaa tcagtaaagc tcccgtgctt ttggtctatt tttgcacctt atgagaagga
     5881 gtattcaact ggcgctgatg ctgttgttct gttctgcagc aggagcccag gaaaaatggg
     5941 atctgcgcca atgcgtggat cacgccgtta aaaataacat ttctgtcagg cagaccgatc
     6001 tgcaatcccg cttttctgcc ctgacttaca gtcagagtaa atcagcccaa ttgcccagct
     6061 ttaattacag tggtagtgtg ggataccggc tgggccgttc ggaaaacccg accaccggtg
     6121 tgctggaaga caataatttc ctgaatctgg gcatgcagct gcaaagcggc gtcacggtgt
     6181 tcaactggtt tgcccgcaag aataccattg agtccaaccg gctcagctat gaagcggata
     6241 agcaacaaac agaaaaaata aagaatgata tttccctgaa cgtggccgtg gcttatctgc
     6301 aaatcctgct cgcccgtgag caggccaata ttgcagcggc ccaggttgaa ctgtcccttt
     6361 cacaactcga aaatacccgt aagcgggtgg aagcaggctc tctgcctgaa ctcaatgctg
     6421 ctgaactgga agcccagctc gcccgtgaca gttcctctct gatcaccgcg caggccaatg
     6481 ccgttcagtt tttactccag atgaaatcgt tgctgaacct cgatgccggt cagcctttcg
     6541 atatagccac accgccggtc aacctgattc ccgtggatcc gattgcggat ttgttgccgg
     6601 aagcagtata tgcctctgcc attaaattta tgccacagca aaaaattaat gaactgcgga
     6661 tcctggccgc aaaaaagaat gtggatgtgg caaaagcagg tctctatccc agttttacag
     6721 cttttggcag tttgtccacc aatgccatta atttccgccg gcccgtttat aaccaggttt
     6781 ttaacgggta tgttccaacc ggtgcccggg tggatgccgg gggtggcact ttttatccgg
     6841 tagaagtgcc agattacagt gatggtacaa cggtagtccg ttatttcaaa cccggcagca
     6901 ttaccaaaca attcaatacc aactttggtc agagtgtcgg aattgggatc agtgttccca
     6961 tattaaatgg caaagcggcg catacggcct gggatcgttc caaagtcagt ttacaattac
     7021 aggaactgca aaaagaacag ggcgatctgc aactgaagca ggatatttac aaagcctata
     7081 atgatgcgac tgctgccctt cagaagtaca atgccgatgt aaaagcgatg cagaccgctg
     7141 aaaaagccta ttcctttgcc agcaaacggt atgaactgaa tctgctttcc gcctatgaaa
     7201 tggtgaccag ccagaccaat atgcaacggg ccaggattca ggcgctttat tcgcaatacg
     7261 attatgtttt taaaatgaaa ctccttgagt tttacaaagg gcagggatta aaactttaaa
     7321 atttggacga tgaataagaa actgaaatgg gtattaatca tactgggtat tctggccttg
     7381 atattcatta ttatgaaagt cgccggcggt ggtgacaaaa cggttaaagt cgcaactgaa
     7441 aaagcggtga agcgtactat tgttgaaacc gtgaatgcca gtggtaaaat atatccggaa
     7501 gtggaagtaa agatcagtcc ggacatttcc ggtgaaatca ctgaactgaa tgtggaagag
     7561 ggtgacagtg tgcggaaagg ggagttgctt gcccgtatct atgctgacat ttatgccctg
     7621 caacgggatg aagcggcttc cagggtgaat cagtcagctg ccacggtgga taacagccag
     7681 gccgccattg aatcacttaa agcgaatctt gaactggctc agcaaagcta taaccgtaat
     7741 aagaaactgt ttgatgaaaa agtgatttcc aaatccgaac tcgaacagtt tgaaaccacc
     7801 ctccgttctg cccaggccaa tttcaatgcg gcccagcaga atattaaaag cctgcaggca
     7861 ggtgtacaaa gcacgcaggt tggactcagc aaagcgaata aagacctgag ccgtacatcc
     7921 ctggtagctc cgatggatgg ggtaatatct tcgttaaaga tcaaaaaagg cgaacgggtg
     7981 gccggtaaca gttttaatgt gggtacggaa atgatgacgg tggccgacat gtcggtactg
     8041 gaagtacggg tggatgtggg cgaaaatgat attgtgaaaa tcaatatcgg ggattctgcg
     8101 gatgtggaag tggatgcgta caataaccgc aaattcaaag gggtggtaac caagattgcc
     8161 agcagcacca aggccggctc gctgacatct tccaatgatg taaccaatta tgaagtcagg
     8221 atccgcctgg acaaggattc atataaagat ctcagtagcc ggactttccc tttccgtccg
     8281 ggtatgaatg caagtgctga tattaaaacc cgccgtgtgg agaatgtgct ggctgtgccg
     8341 attgccgcgg taagtgcgcg ggtaaaagga agcgaccaga gtatggcgga taagaaaaag
     8401 gaaaatgaag ccagtaagga tgagaatgaa gcccagaatc agaatggtat agtggtggaa
     8461 agtgatgaac tggaggaagt ggcttttgtt ttgctcccgg acgggatcgt acaaaaaaga
     8521 gtagtgaaga ccggtgtgca ggatatcagt tatatcgaaa tcctgagtgg attaaaagaa
     8581 ggggaggaac tgatcaccgc cccgtatgct gccgtcagta aggacctgaa agatggcatg
     8641 aaagtaacgg tggtgccgaa ggataaactg tttgataaaa aatagtgtat gcggtttgga
     8701 gtaatcggaa gcggaagctg ggccaccgct cttgccaaaa tcatcacgga taaaaagcag
     8761 ccggttaact ggtgggtccg caatgcggag acgatcgctt tttttatgaa aaaaagccat
     8821 aatccccatt acctcagctc cgcccggttt gataccagct tgctgcatct ttccactgat
     8881 gtacgggaag tagtgcgcaa ttcagatgtg ctggtgattg ccattccttc ggcctatgcg
     8941 gcttcgagcc tggccggact tgccacagaa gactggaagg ggaagaaagt aatatcagcg
     9001 atcaaaggct tgctgccgga gaaaaatatc ctgctcaact attatatgca gcaggaattc
     9061 ggattgggac tggaggatta ttttgcggta cttggtccct gccatgcaga agaagtggcg
     9121 gaagaaaaat tatcctatct cactttttcc gggattgata ccacaactgc cgcccgcatg
     9181 gcggagcatt tccggacaga ttatatcaac acggtggtga atcatgatat cctgggggta
     9241 cagtatgcgg cggtattgaa aaatatttac gcgttagggg ccggtatcgc tcatggactt
     9301 gattatggcg ataatttttt aagtgtatac attgccaatg cggccgatga aatggcggtc
     9361 tttcttcgtc agtttggtgc ggagcatatt acagtaggtg aacatgaagg ggaggatccg
     9421 ctgacccacc gcaagacccc taattatgcc gcatcagttt acctgggcga cctgctggta
     9481 acctgttatt cgttacacag ccgcaaccgc cgctttggga atatgattgg ccggggctac
     9541 acggttaaat ccgccgaact ggaaatgaat atggtggcgg aaggctataa tgctgcccgt
     9601 tgtatccatc ttatcaatca atcactgcaa gccccgattc ccattgctga aacgatctat
     9661 aaaattctct gggaagaggt aaaagcagcg gaaggtttca aaaacatcga agaagtcctc
     9721 gtctgacaac tgccgattta tcctcagaag ccctgtcttc cggcaggcag gtttagcgaa
     9781 ggagggctgc caactaccaa ctgccaactg ccgactgcca actgccgact gccgactgcc
     9841 gactgccaac tgccgcctgc caactgaatc tggtttctta tctttattct gcaatcccca
     9901 ccgccatgat cgcatttttc caggtcctct tttttatcgc ctcgctggta ttgctggtga
     9961 tgaatttcct tgatttaaac aagtggaatg aaagccgcaa atccggagcg gtgaaggcat
    10021 atccgatccg ctggtttaca tttatcctca cctcattagt agcgttgggt tgttttctga
    10081 atatactggt cagctggatc agtaaggctt aaaagaaaag atcagcggcg attccgtcag
    10141 ccagtagttt gccttctctg gtcagtatca gatgatcctc ccggaatagc aggagtccgc
    10201 tatttagata ctttgcagca cccttttcca gttgtttttg ttgcgcttca ttcatccgtt
    10261 gcaggtgtag tccttccatt gtacgtaagg aagtcatgat atattcattt gcctgctggg
    10321 ccggggtcag tatttctttt tcaaaaggaa tacagtcttc ctgcaaggcc ttgatatagg
    10381 tgttattatt ggcaatattc cattgccgtt cattgccata aaaagaatgg gcagatggtc
    10441 cgagtccgag gtagggctgc tgcttccagt atgaagagtt atgccggctg cgcttgccgg
    10501 gaaccgcaaa attagaaatc tcataatgtt cataaccggc ttcttccagc cagtgcatca
    10561 gttgtaagaa ctgtgacgat tgctgatccg gatccacatt ttcgtattgt tcgttacgga
    10621 tcagtttgtc cagtggtgtt ttgggttcca ccgtcaatgc ataacaggac aaatgcggaa
    10681 tacccagggc tatggctttc gccacattct gttgccattt ttcgtcagtg agtcccggtg
    10741 tgccataaat caggtcaatg gtgatattgt caaaatagcg aagcgccagt tccagccctt
    10801 ctgttgcctg cgatgaagta tgggcccggt tcatccagcg caggtcttct tcaaagaaac
    10861 tctgcacgcc gatacttagc cggttgatcc cggcggattt ccaggcctgc aacttttgtt
    10921 ctgtcaggtc atccggattg gcttcgagcg taatttcggc atcgggcaaa agattataat
    10981 gagtgcgtat ttcaacgatc agttgattca cttcttcatt ttcaagcagg ctcggggtgc
    11041 cacccccaaa ataaaggctg ctgactgact ccatgccggg aaaggattgc tgcagcctgc
    11101 tctctttcaa taaagcagcc agtaattcgt tcttataatg cagggaagtg ctgaaatgga
    11161 agttgcagta gtgacaggca cttttacaaa atgggatatg aatgtaaatg cctgccaccc
    11221 gtttgttttt aattgaataa tttagctgac ctatcaaccg gaaatccgct gaaaagccgc
    11281 atgagaaaat acagactgca aatatcggta ctgctggcct tgcttacagc tatttgtacc
    11341 cagggtcagg agaaatatac cctgcgggtc cgttatgcag attccctttc ggctcttatg
    11401 gcagacactt ccctggttca acaaacattc tcaacccgta ttgattgttc caactacctg
    11461 gcccagcttc cggcccgttt acaggcgaga gggtttgtaa cagcttcgct cgatagtatc
    11521 cggtttgaca gtacgtatgc agcaattgta cttttcctgg gacaacgtta ccaatgggcc
    11581 cggctcgata cccggctggt agaacccgga ttgctggagg cggtgggatg gcgggaccgg
    11641 aattttgcgg acaagccaat ggattttgta caagtacgga actggcagga aaaattcatg
    11701 gactacctgg aaaaccatgg atatcccttt gcgaagatat tcctggacag cctgcaaatg
    11761 gatcaggata aagtcagcgg gatattaaaa gtggataaag ggcccctgta taaaattgac
    11821 agcatccggg tatatggaaa tgcgaaaatt acttctgctt atctgcaacg ctatcttgct
    11881 attcccaatg gcagccggta tgaacgggaa aagctgatgc gcatcaatca gaagatccgc
    11941 gagctggcct atgtggaaga agaaaagccc tttgatatca ccctcctggg cacaggttcc
    12001 gtggtgaacc tctatctgaa gcaaaagaaa agcagccagg tcaatgccct gatcggattt
    12061 ttgccgaata atgatcagtt gtcgtccaaa aaactattgc tcacgggaga agccaatatc
    12121 ctgttgcgca attcactggg agccggggaa accatcgggc tcaactggca gcagttacag
    12181 gtgaagtcac cgcggctgaa tatcatatac cagcatccct atttatttca gtccgcggtc
    12241 ggactggatt ttgcgtttga tatgttccgg aaggacagca gttttgtgaa tgtaaatctg
    12301 cagttgggtg cccagtatgt gcttaataaa aatcaatcgg gaaaattgtt tatccagcgt
    12361 ttccagacca tcgtcagtga aggggggata gattcatcgt atgtggtcac ctaccgccgc
    12421 ctgccggacc tgggcgatgt cagttctttt aatgtgggta tcgattacga attgaataat
    12481 acagattacc ggttgaatcc ccggaaaggc aatgaactca ggataatcgg ttccgtgggt
    12541 acgaagacgc tgaaaaagaa taacagtgtg cttgaatata aagaccctgc tgatccgttg
    12601 tttgacttcg ggactttgta tgatacggta aaactgaaaa cctatcagtt ccggttgcgg
    12661 ctgac
//
LOCUS       DAOPMW010000059        12659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5144_length_12659_cov_11.067042, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000059 DAOPMW010000000
VERSION     DAOPMW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_12659_cov_11.067042"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(513..1133)
                     /locus_tag="PLU11_04890"
     CDS             complement(513..1133)
                     /locus_tag="PLU11_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58481.1"
                     /translation="MISVSIIEDDQSYREGLEDFINASGEFRVLNSYPSAETAYPHII
                     EHPPSIAIVDIKLPGASGVDLIYRIKKMRPGIQCMVCSFYDDNEFIFNALRNGASGYL
                     LKDSLAHEIIDSLRELYGGGAPMSRYIARKVISVFQEKPEVPNLSELTERENEILKLI
                     ATGVAIKEAAAELHLSAHTVTKHLKNIYTKLHVNNRIEAVNKLNQR"
     gene            complement(1207..1452)
                     /gene="yidD"
                     /locus_tag="PLU11_04895"
     CDS             complement(1207..1452)
                     /gene="yidD"
                     /locus_tag="PLU11_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPN58482.1"
                     /translation="MGKEIIRVLGRILSIPFLIVIKIYQWIISPWLGPKCRFTPTCSH
                     YAAEALKKHGLFKGFWLSLRRISRCHPWGGSGYDPVP"
     gene            complement(1445..1825)
                     /gene="rnpA"
                     /locus_tag="PLU11_04900"
     CDS             complement(1445..1825)
                     /gene="rnpA"
                     /locus_tag="PLU11_04900"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPN58483.1"
                     /translation="MAKQFTLGRQERLKSRKSIDQLFSEGQRFSQPPIRILFRKTTGT
                     GLKFGVAVSSKLFKKAVDRNRVKRVVREAWRLQKNEFEKQLMEKGQGLDVFFIYTDKE
                     LPVSAVISKATGLIIRKLNERVNG"
     gene            complement(1907..2062)
                     /gene="rpmH"
                     /locus_tag="PLU11_04905"
     CDS             complement(1907..2062)
                     /gene="rpmH"
                     /locus_tag="PLU11_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPN58484.1"
                     /translation="MKRTFQPHRKRRKSVHGFRKRMETANGRKVLASRRAKGRKKLTV
                     SDERKLK"
     gene            complement(2307..3146)
                     /gene="murI"
                     /locus_tag="PLU11_04910"
     CDS             complement(2307..3146)
                     /gene="murI"
                     /locus_tag="PLU11_04910"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPN58485.1"
                     /translation="MPQRNPIGIFDSGYGGLTVMKEIVHQLPQYDYLYLGDSARSPYG
                     NRSFDTVYQYTLQCVKWFFDQGCSLVILACNTASAKALRTIQQNDLPKLAPDNRVLGV
                     IRPTAEIIGQFSETGKVGVLATNGTVASESYPMEIGKFFPELTVIQEACPMWVPLVEN
                     NEQQSKGAEYFVKKNLENIFEKGEDIDVILLACTHYPLLKETIEEYLPVGVKLLSQGE
                     IVAESLADYLQRHPEIEGRCSKEGRHSFYTTDSTTDFDQHATVFFGQEVQSKHVDLDA
                     AVK"
     gene            complement(3371..3895)
                     /locus_tag="PLU11_04915"
     CDS             complement(3371..3895)
                     /locus_tag="PLU11_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPN58486.1"
                     /translation="MKKILSVIAIVGLLAFSGTANAQIKIGYIKIDDVVSLFPETAKI
                     DSVLERYQSDSLNPEYASIVTNYQFNDSLYRDSLHTPKAVRDEIAKKLPTLIYQIQNW
                     QQIVNQALEAKQNEILSPLYRKAYDAIKVVAKEKGYTHVLSKEAFLVAPDGDDMLALV
                     AAKLKLKIPPTAPK"
     gene            complement(3968..4477)
                     /locus_tag="PLU11_04920"
     CDS             complement(3968..4477)
                     /locus_tag="PLU11_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPN58487.1"
                     /translation="MKKILLLACMLGLGFAGISQKYAIIDTRYILDKMPEYKEAQKQL
                     DNIAAGWQKEIDAKQAELDKMYKDYDAEQVMLTEELRKKREDQLFNKEKDLRDLQRKR
                     FGFEGDLFKKRQELIKPIQDKVYNTVQKMAVSRGYDFVLDKSEGITIIFADPKLDKSE
                     DVLKDLGVK"
     gene            complement(4503..7244)
                     /locus_tag="PLU11_04925"
     CDS             complement(4503..7244)
                     /locus_tag="PLU11_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPN58488.1"
                     /translation="MQRFLQRFGIIVLLAVLTGFAANAQETPDTTNPTSVDPKLLDWT
                     TARIAKEYTIGGVNITGIHYLDTSIVYSIANIQPGDKFVHPGAEVFGKAITNLWRQKL
                     FANVQIFVTRIEDDKVWIEIAVQERPRLGNYKFYGVKKSEEEDILGKVTLTKQTIITE
                     NIRRELKEKITQHFADKAFRNVTVRIEEKPDTTYINSNILNIYVNKGDKVRIGSIGFY
                     GNENIDDLRLKKQLKGTKEMSRFTFYPDKSVSPYGENKPFAFNQYMRDWGFLSISKTK
                     ALLDPYFRFKLLSSAKFDEKKYEEDKDNILDYYNSMGYRDAQIVADTQYRTAAGNLNV
                     DLKVKEGRRYYFGNIVWKGNSKYTDSLLTVILGINKGDIYNVAILNKRLGKEAAQEGG
                     DISGLYMDDGYLFFRAEPIETAVYNDTIDYEIRIMEGPQARIKNVTIAGNDKTKDYVI
                     RRELRTVPGELFSRSDLIRSQRELANLQYFNQETINPGVVPNADDGTVDINWKLEEKS
                     SDQLELSAGWGGGIGLTGTLGVTFNNFSIRNIWKKSAWDPLPTGDGQKLSLRLQSNGR
                     AYRSYSASFTEPWLGGKKRNSLTLGFNNSKYSSAYNPYTGTIDRAKSDTVFLKTTGIS
                     LSLGKQLKWPDDFFTLVYTLNYTRYKRQNYGIFQGASPTGVSDNLSFKIALQRSSVFN
                     PIFPTSGSNFLASLQFTPPYSLFNPDIVTSVDPYKTPEYHKWRFNAEWYVPIGKPMGA
                     EKNRQFVMKIAAKYGFMGRYNKKLEYSPFERFQVGDAGLTNNFGLLGYDIIAHRGYPV
                     YESSDPTVNPDQQNASQFFTIFNKYQLEMRFPLVTNPSSTIYALTFFEAANGWYNYKD
                     YNPFRLRRSVGVGMRFFLPMFGLLGFDYGVGLDRIQPGQTGLKGASRFTFMLGFEPE"
     gene            complement(7389..8150)
                     /locus_tag="PLU11_04930"
     CDS             complement(7389..8150)
                     /locus_tag="PLU11_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPN58489.1"
                     /translation="MSALLEKIDKSRLPRHIAIIMDGNGRWAKEKGEDRLFGHFHGVE
                     SVRNIVEGAAELGVEYLTLYAFSTENWDRPEYEVSGLMELLVSTIRKEVESLNKNNIR
                     LHVIGDMSMLPEYARTELNEALDITRPNTGLNLVMALSYSGRWELLNAVKNIAHEVKQ
                     GKLNVEEISQDTLQHFLCTSEFPDPELMIRTSGEFRISNFLLYQLAYAELYFTNVRWP
                     DFRKENLYEAILDYQNRERRFGKTSDQLDETKAPA"
     gene            complement(8365..9222)
                     /locus_tag="PLU11_04935"
     CDS             complement(8365..9222)
                     /locus_tag="PLU11_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPN58490.1"
                     /translation="MKKKILAFVLSLCVVTGTVKSQDAVVQEGEFGFGAGAAHYFGDI
                     NTQARLNRAKFAATVFFRKNFGNYIAGRVGLSFAQLGYSDIYNTHSKVLPTRNLSFNS
                     KVWELTLQGDFNFFRFMPGEPGFSFTPYITFGAGVFNYDPYAYLRGEKIYLRPLGTEG
                     QGSALYPDRKPYSTMALCIPFGGGIKYALNSKMNIAFEVLHRFTNTDYLDDVSKTYVS
                     AATFPNNPDGSPSNAALLSDRSWELGTPIGTDGMQRGNSRQKDQFVTAMFHLTFNLQS
                     YKCPTSSGY"
     gene            complement(9287..10165)
                     /locus_tag="PLU11_04940"
     CDS             complement(9287..10165)
                     /locus_tag="PLU11_04940"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPN58491.1"
                     /translation="MKAAIYSRIFDAEHKEEVQLFFDELELMQIEPVIYQPFYEQIKT
                     DIRIPAETQTFQLSEDLTDEIEFIISLGGDGTLLDTITLVRDKKISIMGINFGRLGFL
                     ASIGRNEVKSAVKAIARRTYVNDSRTLIHVDADMPLFGNIPYAMNEFSIHKQDVASMI
                     KIHTYLNGEFLNSYWADGLIVATPTGSTGYSLSCNGPVVFPDSGSFVITPVAPHNLNV
                     RPIVVPDTNIISFEVESRTDKIICALDSRREIVHKNVTLAVKKEDFRINLVRLSENNF
                     LQTLRNKLTWGLDKRN"
     gene            complement(10167..11603)
                     /locus_tag="PLU11_04945"
     CDS             complement(10167..11603)
                     /locus_tag="PLU11_04945"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPN58492.1"
                     /translation="MSKLKKYYPALILALLICNGLCAQLASLTDMPADTSVAPSAAKY
                     IIGNIFITGNKKTRDPIILREIPVRTGDAYSLSDLVKKFEDARRQLMNTTLFHSVLVA
                     AKGFRGNVIDIDVSVKERWYLFPLPYFKPVDRNLNQWLVEQKASLSRVNYGAKLLYNN
                     ATGNNDKLRLWLVSGYTKQLSLGYDRLYIDKAMKWGMRFNFAMGKNRELNYNTIDDKQ
                     VFLKDNDRYVRSFVNTQAELTYRRAINTRHSIGVGYTSEEVEDTIVQLNPSYFKAGRN
                     RLRFPEIYYNMTYYNVDYIPYPTKGYAANIMLRKSGINRTNNLWQLQLKGLGSWHLSP
                     KSFLSLSAFAGIKAPFKQPYFNTRFLGYGDVFMQGYEYYVIDGVAGGYLKATFAREFL
                     NFSIRIPKNKRGTADMIPFRVYGKLYGNSGYVHNPQPGENALSNTLLHSAGIGIDIVT
                     FYDVILRFEYSFNQLGQNGLYLHRKSIF"
     gene            complement(11672..12334)
                     /locus_tag="PLU11_04950"
     CDS             complement(11672..12334)
                     /locus_tag="PLU11_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPN58493.1"
                     /translation="MLTRELESQSLPYLHLTDKVYQALQLMNDNHVTHLPIVEGDKYS
                     GLISEDDLLMADNDHAELGTLQQSFANSAVKGHEHFLKAVQVAADNGLSVVPVINEEG
                     ELISTVAYNDLLKQASEFMSLNEPGGLIVLEVDSNQYSFNEISKLVETNDAQITQLNT
                     SNDPETGIMQVTIRINKPEVSDIIATFQRYEYNVKYFFGEELYTNELKSNYDNLMNYL
                     KF"
     gene            complement(12404..>12659)
                     /locus_tag="PLU11_04955"
     CDS             complement(12404..>12659)
                     /locus_tag="PLU11_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN58494.1"
                     /translation="AKSAIRNNLGEELNQIKQPTLLVWGNNDTITPPFVGREFNKLIP
                     NSELHFVDKCGHAPMMEVPDEFNAILHKFLKKLNEPAAVA"
BASE COUNT         3255 a         2888 c         2958 g         3558 t
ORIGIN      
        1 tgaaaagctg gctctgttag ggccggcttt tttttagttc aaccccttcg gggttaggag
       61 ctgaaatcat aatcaataac ctgcacccat gtggttcctg taaaatatta gtattgaatg
      121 ctgaggaatt atcaatccct cggggatgaa cgtattgata attttgaatc tccggcgaaa
      181 aaaatacggt taattatact ttctcctatt cgagacattt ttaaattaaa ggataataca
      241 ggtaattaag ataaatagcc cgaagggctt aaatttttaa tagccccggg tgaaacccgg
      301 ggtaagggca cataatgacc cttccaaccc cgaaggggtt gaacaaaaga atacagcaat
      361 aaacattatg tacgcactga aagagaaaat tattttaaag acagggaaat ttttacttgc
      421 ataaccatat tacaagtgtg aaacaaaacc ttcggcgatc tttcgtactt cgtactttgt
      481 atttttcgta attcgtactt cgtacctcgt acctatctct ggttgagctt attcaccgcc
      541 tcaatccggt tattgacgtg tagttttgtg tagatattct tcaggtgttt ggtcaccgta
      601 tgtgcactta aatgcagttc agccgcagct tctttaatag ctacgcccgt agcgatgagt
      661 ttaagaattt cattttctct ttcggtgagt tcggataaat tcgggacttc cggtttttcc
      721 tggaaaacgg aaattacttt gcgggcaata taacggctca tcggggcgcc acctccatat
      781 aattctctca atgaatcaat gatctcgtgg gccagcgaat ctttcagcag gtaaccactg
      841 gctccattac gaagcgcatt aaaaataaat tcattgtcat cgtaaaagga acaaaccatg
      901 cactgaatgc ccggccgcat tttttttata cggtaaatca gatccacacc actggctccg
      961 ggtaatttaa tatccacaat agcaatggac ggcggatgtt cgatgatatg gggatatgct
     1021 gtttcagcag agggataact gttcagtacc ctgaattcac cagaggcatt gatgaaatcc
     1081 tcaagccctt cacggtagct ctgatcatct tctatgatgg aaacagatat caattcacac
     1141 actttaaaca gcaaggtact gaataatcag tgaccatcta tgaatgatac gttaatgcaa
     1201 ccttaatcag ggaacgggat catatccgct tccgccccag gggtgacaac ggctgattcg
     1261 tctgagtgac aaccagaaac ctttaaagag accatgcttt ttcagggctt ctgccgcgta
     1321 atgggaacag gtgggcgtaa aacggcattt gggtcccagc cagggtgaga tgatccattg
     1381 atagattttg atcactatca ggaagggaat actcaggatt cgccccagca cccgtatgat
     1441 ttccttaccc attgacccgt tcatttaatt tacgaataat aagaccggtg gccttgctga
     1501 taacagcgga tacgggtagt tctttatccg tataaataaa aaacacatct aatccctgcc
     1561 ctttctccat caattgcttt tcgaattcat ttttctgtaa ccgccaggct tcccgtacta
     1621 cccgtttgac ccggttgcgg tccacggctt ttttgaagag tttactactc acggccactc
     1681 caaactttaa accggtaccg gtggtcttac gaaataagat ccggatgggt ggttgggaaa
     1741 agcgttgccc ttcactgaac aactgatcaa tggatttccg gcttttcagc cgttcctgcc
     1801 tgccaagagt gaattgtttt gccacggtat aaaaaaaggg ctgtacccat aaagatacag
     1861 ccctgtaaaa ttatcgaaac cgccgttgaa acaacgggga acctttttac ttcagcttac
     1921 gctcatcaga aacagtcagc ttcttacggc ctttggcacg acggctggct aatactttac
     1981 ggccattggc agtttccata cgtttacgga aaccatggac cgacttacga cgcttacgat
     2041 gaggttggaa tgtacgtttc ataattattt cgctttttca cttttattct ccattgctgt
     2101 tgctccggaa aattttgaaa tatggaccgg gagaacggcc ctggtttcat ttcgacaacg
     2161 ggtcaccaca cccactgttc gtgaaaggac ggcaaaggta agggttggga agcagaatag
     2221 gaaaagaaga cggattattt tacccggtac cggcggggtg ttttatgaaa atcaaagtga
     2281 caaatgagcc tataattaat tgattttcat tttactgcag catccagatc cacatgtttt
     2341 gattgcactt cctgtccaaa gaaaaccgtg gcatgctggt caaaatcggt ggtggaatcg
     2401 gtggtataga aactatggcg gccttctttg ctgcagcggc cttcgatttc cgggtgccgt
     2461 tgcaggtaat ccgccagact ttcggccaca atctctcctt gtgaaaggag tttaaccccg
     2521 acaggcaggt attcttcaat ggtctctttc aataagggat aatgggtgca ggccagcagg
     2581 atcacatcaa tatcttctcc tttttcaaat atgttttcca ggttcttctt tacaaaatat
     2641 tcagcgcctt tactctgctg ctcattgttc tctaccaggg gcacccacat aggacaggct
     2701 tcctggatca ccgtcagttc agggaagaat tttccaatct ccatcgggta ggattcagat
     2761 gccacggtac cgttcgttgc gagtacaccc actttacccg tttcggaaaa ttgtccgatg
     2821 atttccgctg ttggcctgat cactcccaaa acccggttat caggtgccag tttgggcagg
     2881 tcattctgct gaatggtacg aagggctttt gcagatgccg tattgcaggc caggatcacg
     2941 agggagcaac cctggtcaaa aaaccatttc acgcattgaa gggtgtattg gtacaccgta
     3001 tcaaaggacc ggttcccata gggcgaacgg gcgctatcgc cgagatagag atagtcgtat
     3061 tgcggtaact gatgtacaat ttccttcatc acggtaagtc cgccataacc ggaatcgaat
     3121 atacctatgg gattgcgttg aggcataaga caaagttagt agttagtagc cggtagttag
     3181 tagtccgatg cttcggacaa tacacctcct gagtgaaata acttaatccg tgtttatccg
     3241 tgttatccgt ggcctgcctt tctgtattca ttcgtgagtt ttcgtgtgat ttcgtggcca
     3301 cccctccttg ccgcaggcaa aaaaaagccg tcccggatac cgggacggct ttcagtattt
     3361 tattcaaccc ttatttagga gctgtcggag gaattttcag tttcagcttg gcagccacca
     3421 gcgccagcat atcatcacca tccggggcaa ccaggaaagc ttctttgctt aacacatggg
     3481 tgtatccttt ttccttagcc accactttga tagcatcata ggctttgcgg taaagcgggg
     3541 acaggatttc attttgcttg gcttccaggg cctggtttac aatttgctgc cagttctgga
     3601 tctgatagat cagggttggc agtttcttgg caatctcatc acgtacggct ttgggcgtat
     3661 gtaaagagtc acggtaaaga ctatcattga actgatagtt ggtaacaata gaggcatatt
     3721 caggattcag tgaatcggac tgatagcgct caagaacaga gtcaatttta gccgtttcag
     3781 gaaacaggct taccacatca tcaattttga tatagccgat tttaatttgt gcattagcgg
     3841 tacctgagaa tgccagcaaa cccacgatag caatgactga aaggatcttt ttcatctcga
     3901 tttttttgtt atgctttata tgatactaaa aaaaggcagt tagttgcgcc ttaactttct
     3961 ttaacattta ctttaccccc aggtccttca gcacatcctc actcttgtcc agtttggggt
     4021 cggcaaatat aatggtaatt ccttcacttt tatccagtac aaaatcatag ccccggctca
     4081 cggccatttt ctgaacggtg ttgtacactt tatcctgaat gggtttgatc agttcctggc
     4141 gttttttgaa cagatctccc tcaaaaccaa agcgtttgcg ctgcagatcc cggaggtctt
     4201 tttccttatt aaagagctga tcctctcttt ttttccggag ttcttcggtc agcatcacct
     4261 gctcggcatc ataatccttg tacattttgt ccagttcggc ctgtttagcg tcgatttcct
     4321 tctgccagcc ggccgcaata ttgtccagtt gtttctgggc ctctttatac tccggcatct
     4381 tgtccagtat gtagcgggtg tcgatgatcg catatttctg agaaataccg gcaaagccca
     4441 gtcccagcat acaggcaaga agaagtatct ttttcatata gaggaatttg tggttaaaca
     4501 atttattccg gctcaaagcc cagcataaag gtaaagcggg atgctccttt cagaccggtc
     4561 tgaccgggct ggatacgatc aagtcccacg ccatagtcaa atccaagcag accgaacatt
     4621 ggcaggaaga aacgcatacc tacacccacg cttctgcgta aacggaaggg gttataatct
     4681 ttatagttgt accagccatt ggccgcttca aagaaggtca gcgcataaat ggtactgctc
     4741 gggttggtca cgagcgggaa acgcatttcg agctggtatt tattaaagat ggtgaagaac
     4801 tgactggcat tttgctgatc agggtttact gtaggatcag aggactcata caccgggtat
     4861 ccacggtgag cgatgatatc gtaaccgagt aagccgaagt tattggtcag acccgcgtca
     4921 cccacctgga aacgttcaaa tggtgagtat tccagtttct tgttgtagcg gcccatgaaa
     4981 ccatacttcg cagcaatctt cattacaaac tggcggttct tttcagcacc catcggttta
     5041 ccaatcggca cataccattc tgcattaaaa cgccatttgt gatactcagg ggttttatag
     5101 gggtccactg aagtaacaat gtccgggttg aataatgaat acggtggggt gaactgtaag
     5161 ctcgccagaa aattggaacc actggtcggg aagatcgggt tgaacacaga agaacgctgt
     5221 aaagcgatct tgaagctcag gttatccgat acaccggttg gactggcgcc ctggaagatg
     5281 ccatagttct gacgcttata acgcgtatag ttcaatgtat aaactaaggt aaagaagtca
     5341 tcgggccatt tcagctgctt acccagtgat aaagagatac cggtggtctt caggaaaacc
     5401 gtatctgatt tagcacggtc aatggttccg gtataaggat tataagcact ggagtattta
     5461 ctgttattga atccgagggt cagtgagttt cttttcttac cgcccagcca gggttccgta
     5521 aaggaagcac tgtaggaacg ataggcgcgt ccattggatt gtaaacggag acttaacttc
     5581 tgtccatctc ctgttggaag gggatcccag gcggatttct tccagatatt ccggatggag
     5641 aagttgttaa acgtcacccc aagtgtaccg gtcagaccga taccaccacc ccaaccggca
     5701 gagagttcaa gctggtctga agatttctct tccagtttcc agttgatatc cacggtaccg
     5761 tcatcggcat tgggtactac acctggattg atggtttcct ggttgaagta ttgcaggttg
     5821 gccaactcac gttgggagcg gatcaggtct gagcggctga acagttcacc gggaacagtg
     5881 cgcaattcac gacggatcac ataatctttt gttttatcat taccggcaat agtaacattt
     5941 tttatacggg cctggggacc ttccattata cggatctcgt aatcgatggt gtcattgtat
     6001 accgctgttt caatcggctc ggcacggaag aagaggtagc cgtcatccat gtacagtccg
     6061 ctgatatcac cgccttcctg ggcagcttct ttacccagac gcttattcag gatggccacg
     6121 ttatagatat cgcctttgtt gattccgagg atcacggtca gtaaggagtc ggtatatttt
     6181 gaatttcctt tccagacgat atttccaaaa tagtagcggc ggccttcttt tactttcagg
     6241 tccacattca gattgccggc tgccgttctg tattgggtgt ctgctacgat ctgagcatcc
     6301 cgatatccca tggaattata ataatcgagg atattatcct tgtcttcttc gtatttcttt
     6361 tcgtcaaatt tggcactgct cagtagtttg aaccggaaat agggatcaag cagggccttt
     6421 gttttactga tggagagaaa gccccagtca cgcatatact ggttaaaagc gaagggtttg
     6481 ttttccccgt aaggactgac cgatttatca ggatagaaag taaagcggga catttccttg
     6541 gtccctttca gctgtttttt cagccggagg tcatcgatat tttcattgcc atagaaacca
     6601 atagaaccaa tccgcacttt gtccccttta tttacataaa tattgagaat attagagttg
     6661 atataggtgg tgtccggttt ctcctcaatg cgtaccgtta cattcctgaa tgctttatcc
     6721 gcaaagtgct gagtgatttt ttctttcagt tcgcggcgga tattttcagt gataatggtc
     6781 tgtttggtca gggtgacttt gccgaggata tcttcttctt ccgatttttt aaccccgtaa
     6841 aatttatagt tccccaggcg cggacgttcc tgtacagcaa tttcaatcca tactttatcg
     6901 tcttcaatcc gggtcacaaa gatctgaacg ttagcgaaaa gtttttgtct ccagagatta
     6961 gtaatggctt tcccaaatac ttccgctccg ggatgtacaa atttgtcacc gggttgaata
     7021 ttcgcgatag agtagacaat ggacgtatcg agatagtgga tgccggtgat gttgacacca
     7081 ccgatcgtgt attctttggc aatacgtgct gtggtccaat caagaagctt ggggtccacg
     7141 gaagtggggt tggtggtatc cggagtttcc tgtgcgtttg cagcgaaacc cgtgagcacc
     7201 gctaacagta ctatgatgcc gaaccgttgt aaaaatcgtt gcatgtgtta ttgttctggt
     7261 ctcagtcccg gtattcggga gtttaattag tttattttcc gcgatttata aaaataccgc
     7321 catcttgaac cggcggcaaa ttaacggtta aaatcgaaag tctttgttaa aagaaagcca
     7381 gtcttccctc aggccggagc ctttgtttca tccagctgat cactggtttt tccaaacctt
     7441 ctttcccggt tctggtagtc caggatggct tcatacaggt tttctttcct gaaatcgggc
     7501 cagcgcacat tggtgaaata caattcagca taggccagct ggtaaagcag gaaattgctg
     7561 atccggaatt ccccgcttgt acggatcatt aattcgggat ccggaaactc actggtacag
     7621 agaaaatgct gcagggtatc ctggctgatc tcctccacat tgagtttacc ttgcttcact
     7681 tcatgcgcaa tatttttcac cgcattcaac agttcccacc ggccactgta actgagggcc
     7741 atgaccaggt taagtccggt attgggccgg gtaatatcaa gtgcctcatt aagttctgtc
     7801 cgtgcatatt caggaagcat actcatatcc ccgatcacat gcagccggat attgttctta
     7861 ttcaggcttt ccacttcctt acgtatagta ctgaccagca gctccataag tccgctcacc
     7921 tcatactccg gccggtccca gttttcagtg gaaaatgcat aaagggtcag gtattccacc
     7981 cccagttccg ccgcgccttc cacaatattc cggacacttt ccactccatg aaaatggcca
     8041 aacaggcggt cctctccctt ctccttcgcc caccggccat ttccatccat aatgatggca
     8101 atatgccggg gcaggcggct tttatctatt ttttcgagta aggcagacat aaaaaaatcc
     8161 ccattaaacg ggggcacgaa ggtaccgtca atttttggga ttccctcctt ttttaaaggt
     8221 aaaagaccta aagaagtaag tacgaagtaa gaagtacgaa gtacgaggaa tggccgtaaa
     8281 acgaggaaaa ctggtttttt tagcctcagg caccttagtt ggggctgttt cctgacaccc
     8341 gacaactaac acccgacacc ctttctagta ccccgaagaa gtcgggcatt tatacgattg
     8401 caggttaaat gtaagatgaa acatcgccgt tacaaactgg tctttttgac ggctgttgcc
     8461 tctttgcatc ccatcggttc caataggggt acccagttcc caggaacggt cgctcagtaa
     8521 tgcggcattg gagggactgc catcgggatt gttgggaaaa gtggcggcgc tgacataggt
     8581 tttacttacg tcatcgaggt aatccgtatt ggtaaagcgg tgcaggactt caaaagcaat
     8641 attcatcttg ctgttgagtg catacttgat gcccccgcca aagggaatgc agagggccat
     8701 cgtactgtac ggcttgcggt ctggatagag tgcactgccc tgtccttcgg tgcccagcgg
     8761 acgtaaatag attttctccc cgcgcagata ggcataggga tcgtaattaa acaccccggc
     8821 cccgaaggtg atatagggcg taaaactaaa gccgggttct ccgggcatga aacggaaaaa
     8881 gttgaaatca ccctgtaaag tcagttccca gaccttcgaa ttgaagctca ggttacgggt
     8941 gggcaatacc ttgctatgcg tattgtaaat atcagaataa ccaagctggg caaaagaaag
     9001 acctacccgt ccggcgatat aattgccgaa atttttgcgg aaaaaaacgg tggccgcaaa
     9061 tttggccctg ttgagccggg cctgtgtgtt gatgtcgcca aaataatggg cagcaccggc
     9121 accaaatcca aattctcctt cctgcacaac ggcatcctgg gatttaacgg tacctgttac
     9181 cacacagagg gaaagcacaa aagctaatat tttctttttc ataactataa aggacgggat
     9241 tcaatagaac gctaaaatta gcaatttctt gtaatcagga cctggtttaa ttgcgtttat
     9301 ccagtcccca ggtgagttta ttacgaagcg tctgaagaaa attattctcg ctgagacgga
     9361 ccaggttgat cctgaaatct tcttttttta cggcgagcgt tacgttttta tgtacgatct
     9421 cacggcggga gtcgagggcg cagattattt tatcggtcct gctctccact tcaaaggaga
     9481 taatattcgt atcaggtact acaatgggcc ggacattcag gttatgcggg gcaaccggtg
     9541 tgattacaaa actgccggag tccgggaaga ccacaggacc gttgcagctg agcgaatagc
     9601 cggtagaacc ggtgggggtg gcaacaatca ggccatcggc ccaataggaa ttcaggaatt
     9661 cgccattgag ataagtgtgg atcttgatca tagaagccac atcctgttta tggatcgaaa
     9721 actcattcat ggcataggga atattgccaa aaagcggcat atcggcatcc acatggatga
     9781 gtgtccggga gtcgttgaca taagtacggc gagcaatggc tttcactgct gacttcactt
     9841 cattacggcc gatactggcc aggaagccca gccggccgaa attgatcccc ataatggaaa
     9901 tctttttatc ccttaccagg gttatggtat cgagcagggt accatcccca ccgagactga
     9961 tgatgaattc aatctcatca gtcaggtctt cagataactg aaaggtctgg gtttctgccg
    10021 gaatgcggat atccgtcttt atctgttcgt aaaagggctg gtagatcacg ggttcgatct
    10081 gcatcagttc cagttcgtca aagaaaagct gtacttcttc cttatgctct gcatcaaata
    10141 tcctgctgta aatcgctgcc ttcatgttag aaaatagact ttctgtgtaa atataatccg
    10201 ttttggccta actggttgaa gctatactca aagcggagga tcacatcata gaaggtaacg
    10261 atatcaatac cgataccggc ggagtgcagg agtgtattgc tgagtgcatt ttcgccaggc
    10321 tgcggattgt ggacatagcc gctgttccca tacagttttc cataaacccg gaagggaatc
    10381 atatcggccg taccccgttt gtttttcggt atacggatcg aaaagttgag aaattcccgg
    10441 gcaaacgtgg ctttcaggta tccgccggcc acgccgtcta tcacataata ctcatagcct
    10501 tgcataaaca catcaccgta tccgagaaaa cgggtgttga aatagggttg tttaaacgga
    10561 gcttttatac cggcaaaggc agaaagggac aaaaacgatt ttggcgagag gtgccagctt
    10621 ccaagtcctt ttaactgaag ctgccagaga ttattggtgc ggttgatgcc gcttttccgg
    10681 agcatgatat tggccgcata tcctttcgta gggtaaggga tataatccac attgtaatag
    10741 gtcatgttat aataaatctc gggaaaacgc agcctgttcc gcccagcttt gaagtaggaa
    10801 gggttcagtt gcacaattgt gtcttccact tcttcactgg tgtacccgac accgatactg
    10861 tggcgggtat tgatggcgcg gcggtaagta agttcggcct gtgtattcac aaaactgcgg
    10921 acatagcggt cattgtcctt cagaaatacc tgtttatcat caatggtatt gtaattgagt
    10981 tcccggtttt tgcccatggc aaaattgaac cgcatgcccc atttcatcgc tttatcgata
    11041 tagaggcggt catacccgag ggataactgc ttggtataac cggaaaccag ccagagacgg
    11101 agtttatcat tattaccggt tgcattatta tacaacaatt tggcgccata gttcaccctg
    11161 gagagactgg ctttttgttc caccagccat tggttcaggt tccggtccac cggtttaaaa
    11221 tacggaaggg ggaaaaggta ccatcgttcc tttacagaca catcaatatc gatcacattt
    11281 ccgcgaaaac ctttggccgc caccagtacg gagtggaaaa gcgtcgtgtt catgagctgg
    11341 cgccgggcat cttcaaattt tttcaccagg tcggaaagcg agtaggcatc accggtacga
    11401 accggaattt cgcggagaat gatcgggtca cgggtttttt tattgccggt aatgaaaatg
    11461 ttcccgataa tgtacttagc cgcggacggg gccacgctgg tatcggccgg catatccgtc
    11521 agggaggcca gctgagcaca gagaccatta cagatcagca atgccaggat cagcgcaggg
    11581 taatattttt ttagcttgct caaaaaaaga tttcctgtat ctacattaaa atacaagtga
    11641 attcaatgct gtaaaatacg ggtaaatggg atcagaactt cagataattc atcaggttgt
    11701 cgtaattgct cttcaattcg ttcgtataca gttcttcccc gaaaaaatat ttcacattat
    11761 actcataccg ctggaaagtg gcaataatgt cggacacttc cggtttattg atccggatgg
    11821 taacctgcat gatgccggtt tccggatcgt tgctggtatt cagttgggtg atctgtgcat
    11881 cattggtttc caccagtttg ctgatttcat tgaatgaata ctggttgctg tctacttcca
    11941 gtacgattag tccgccgggt tcattcaggc tcatgaattc agaagcttgt ttcaggaggt
    12001 cattgtaagc cacggtgctg atcagttcgc cttcttcatt gatcacaggc accacactta
    12061 gtccgttatc ggcggcaacc tgaacggctt tcagaaaatg ttcatgccct tttacggcgg
    12121 aattggcaaa ggactgctgg agggtaccca gttcggcatg gtcattatca gccatcagca
    12181 ggtcatcttc actgatcagt ccggagtatt tgtcgccttc cacaatagga aggtgggtga
    12241 catgattatc attcatgagc tggagcgcct ggtacacctt atcggtaagg tgtaaataag
    12301 ggagtgattg agattcgagt tcccgggtta acatggatag cggctttata tgttattaga
    12361 acggctgcaa caggtggcgg cagttctgtc aagactgcat tgttcaggca actgctgcag
    12421 gttcattcag tttctttaaa aatttgtgca ggatggcatt gaactcatcc ggcacttcca
    12481 tcatcggggc atgaccgcat ttatcgacaa aatgcagttc actgttcggt atcagcttat
    12541 tgaactcgcg gccgacaaaa ggcggagtga tcgtatcatt gttaccccac actaataacg
    12601 taggctgttt gatctggttc agttcctcgc ccaggttatt ccggatggca cttttggcc
//
LOCUS       DAOPMW010000060        12078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5518_length_12078_cov_8.370290, whole genome shotgun sequence.
ACCESSION   DAOPMW010000060 DAOPMW010000000
VERSION     DAOPMW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12078
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5518_length_12078_cov_8.370290"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1452)
                     /gene="mnmE"
                     /locus_tag="PLU11_04960"
     CDS             complement(73..1452)
                     /gene="mnmE"
                     /locus_tag="PLU11_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPN58495.1"
                     /translation="MNKLTGWDDTIIALATPPGVGAIGVIRLSGARAIELVNRLFPSK
                     NLLLQAAHTLHVGLLKEGETVLDEVVISLYRNPRSYTGEDVIEISCHGSPFVQQQVID
                     ACIRAGARLAKPGEFTQRAFLNGKMDLTQAEAVADLIAANTAASQKAALHTIRGGFSE
                     NLKELRDRLLTFSALIELELDFSQEDVEFADRTQFYDLINEMQTATGGLIQSFSLGNV
                     IRNGVAVAIVGKPNAGKSTLLNTLLNENRAIVSEIPGTTRDTIEELINIDGILFRLID
                     TAGIRQHTTDTIESAGIERSLAKIKQADVVLYLFDVNGESTELTNWKKEADEAGLRYL
                     LVGNKIDAGDEAAARLRFSAFEPVVYIAAKEKRHIDVLRERMVDLVLQGQVQAENTII
                     TNARHYHALQQVAGSLHEIKAGLDNKLPGDLLSLDIRRCLHYLGEITGEITNEDQLDY
                     IFSKFCIGK"
     gene            complement(1827..2657)
                     /locus_tag="PLU11_04965"
     CDS             complement(1827..2657)
                     /locus_tag="PLU11_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPN58496.1"
                     /translation="MEANKILSADVLDIIFDGKNKDYGAYELRKTYNRRITRALIITG
                     SVALLALLSSILSSTMKEKADKKLKMNEMTIQDVKQEEKKAIPPPPPPPPPKQEPPKV
                     EMKQFTPPVIKKDDEVEKPPPPQEELKEAKIDVVSQEGIKDEGIAAPVDIDQGKQIVE
                     VKKEDDENKIFDKVEIEASFPGGDSKWRQYLERNANGQVATDNGAPEGTYTTVVQFVV
                     DKEGNISDVRALTNHGYGMEEEAIRVIKKGPKWSPAVQNGRQVKAYRKQPITFRVEAE
                     "
     gene            complement(2714..3340)
                     /locus_tag="PLU11_04970"
     CDS             complement(2714..3340)
                     /locus_tag="PLU11_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPN58497.1"
                     /translation="MASIEEGGDGGHKKGPGVKKAKKLSTRVDMTPMVDLGFLLITFF
                     VFTATMSTPTTLDLNMPKDVKDVKEQTEVKESSVLSIMLGKNNVIYYYEGKLKEDGSN
                     FKTATYTSIRDIIVNKKKEVISRYVTDPACEAKAKAEGKPVSNCADKDFVVIIKPSPE
                     ATYKNTVDMLDEMTINQVRTYAMVKIFDQEFELIKVTEGAAPATAPAK"
     gene            complement(3360..4028)
                     /locus_tag="PLU11_04975"
     CDS             complement(3360..4028)
                     /locus_tag="PLU11_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPN58498.1"
                     /translation="MPSVKIPKKSTFTDMTPFVDVAFLILSFFIMATKFKPQEPVEIT
                     TPGSVLSQKLPENNAVLISIDSTDRVYFTILSEKDKSKFDVIIAGINSSQNLGLSPSE
                     MANFKKTFAVGVPFAGLKQLLATDAKDQPKLKVPGIPVLDTLNNQLFWWIREAKSAFR
                     GEKLNYLIKGDGASKYPTFEAVISALKKNEEFKYNLVTALDEIPAGTELYRREAAGEK
                     DLKK"
     gene            complement(4089..4949)
                     /locus_tag="PLU11_04980"
     CDS             complement(4089..4949)
                     /locus_tag="PLU11_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPN58499.1"
                     /translation="MAEIKPTATASGKATTVQAKKSSNAISWIAPIVCVLAGYAIWRF
                     MMGNAASFEQPDPAGGFWPSHKGPKDAFSRIYEGGIIVPLLIGMLLMVIVFSIERLLT
                     IRKALGNGSIADFIRKVQYHLANKNVDAALAECDKQRGSVGNVMKAGLRRYKEMISEG
                     NLNTEQKVIAIQKEVEEATALELPMLQKNLVFLSTITSVGTLIALLGTVIGMIKSFSA
                     LGNEGGGSAAGELAIGISEALYNTALGIGTSALALIMYNIFTTKIDSITYGIDESGFT
                     LTQSFASLYK"
     gene            complement(5158..6435)
                     /locus_tag="PLU11_04985"
     CDS             complement(5158..6435)
                     /locus_tag="PLU11_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN58500.1"
                     /translation="MNFRDVFSLAFRTVRGNKLRTGLTVSIIAFGIMALIGIITAIQA
                     MNQKFTESFSSMGANAFTIRFKERNIRFGGGNRSEMKLSTRGGRKSRVSNLDKNITKD
                     EAELFVRRFDFPSMKSISIFSNRNNIVSHENFKTSPNVLMFGGDENYLALNGFSLQYG
                     RNMNRMDVQTGRNVCILGYDVAKRLFKQGPAGAVNGVVRINNIPFRVLGVLESKGSSF
                     GFNRDNIIITTYTNVDRNFPSGFSYVIAVKANDLIKVNAAMGEAEGTFRAIRKLMITE
                     ESNFVLDRSDSVAEKAMNSLRFLTISATVIGLITLIGAAIGLMNIMLVSVSERTREVG
                     LVKAIGGKSQMVSRQFLLEAIIISMLGAIFGIILGILVGNSFSLVLNTGFVVPWNWVI
                     YGIVICTLVGLLAGLYPALKAGRLNPIEALRYE"
     gene            complement(6508..7884)
                     /locus_tag="PLU11_04990"
     CDS             complement(6508..7884)
                     /locus_tag="PLU11_04990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPN58501.1"
                     /translation="MNALIISAVLGVVMMFSGILLKQKSAIRTVAIAGILVLLGMSIL
                     DLKGIQLFSIDTREMIVFDHFALVFNTVIFAVVLLYLLLSAPDMEKVGVNYAEYFALI
                     FFILCGATLVTSFKSLLILFLGIEIISIPLYILTGSDKRNLKGNEAALKYFLLGSFST
                     GLMLMGIALLYGARASFSTVISPAIIPQNMSLLYTGMILLLFAMAFKVSAAPFHFWTP
                     DVYDGAPTVFTSFMATIVKVVVFFGFIRLFDDMFEPQQGQWRLWVTVMTAVTLFIGNI
                     TAVFQQSVKRMLAYSSIAQAGFMLFALYAMNGDGKEGLMLYTVAYSAATIGIFAVLAR
                     MSDYTFDGFNGLAKSQPMVAATLTVFLLSLAGIPLTAGFMAKFYMLKAALGTPGSLWL
                     VIFAVLMAAVSVYYYFRVIQAMYFKEPGDTPHPAFPSFFKGMLLVLAFLVILLGVYPE
                     LLLGRLYF"
     gene            complement(7900..9372)
                     /locus_tag="PLU11_04995"
     CDS             complement(7900..9372)
                     /locus_tag="PLU11_04995"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPN58502.1"
                     /translation="MIAFLLILIPLATGLLSFLIREEKTAKAFAFLASLATLAVSLLG
                     LTLMNSESNLQFSAPWMSSLGSSFALKIDGMGQVLCLLTAIAYPLTFLGTWQASPKNA
                     NRFFGLMLLAQAGMMGVFLANDALLFYFCWELALIPMYFLASQWGGEKRIAVTFKFFV
                     YTFLGSLLMLIGILYLQSQTPDHSFGLSAFYKLKIPASDQLWLFWLFFVAFAIKMPLF
                     PFHTWQPDTYEQAPTATTMILSGVMVKMGLLGLIRWLMPVLPLASFQWGDVVMILAVI
                     GIIYASLVAIRQDDLKRLIAYSSIAHISLMAAALFAENLSGTQGVMIQLFNHGVNIIG
                     LWIIVEIIERRYGTRKISELGGLAQQAPVMAIFFVVIALANIALPLTNGFAGEFLMFN
                     GLLSAQSNFKVVFTVLAGLGIILAAVYTLNMIRKTFYGEAVSLRVPVTDLKLNEQLAL
                     GLIVVIILWLGIYPQSMLELTDSFSKEWFSKIDVSYLFRK"
     gene            complement(9400..11301)
                     /gene="nuoL"
                     /locus_tag="PLU11_05000"
     CDS             complement(9400..11301)
                     /gene="nuoL"
                     /locus_tag="PLU11_05000"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPN58503.1"
                     /translation="MNNALQIVYLIPLLPLLGFLINGLGRNYLSKTLIGLIGSGSILG
                     SLILSIWVFMQVKDGNTFVAEYFNFIAVDKLVIPFALKIDQLSAVFLLIITGVGFLIH
                     VYSASYMHEEKPGHFGRYFAYLNLFVFSMLLLVMGANLVILFIGWEGVGLCSYLLIGY
                     WFKKQEYNKAANKAFIMNRIGDLAFLIAIFWLIARVGTVSFDELFTAVNLAKLSASDI
                     TVITLLLFIGATGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIARSHVLY
                     SMDAAELTRNIITYTGMATALLAATIALKQNDIKKVLAYSTVSQLGFMFVALGTGEYV
                     AAVFHVMTHAFFKALLFLGSGSVIHAMGGEQDIRNMGGLSKKLRITYVTFFIGCIAIA
                     GIPPFSGFFSKDAILLSAFMKSPVLYAVAVFTALLTAFYMFRLLIITFMGKFRGTDEQ
                     AHHLHESPLAMTLPLIILAVLSIVGGLVGIPEVIREGGHKLSAFLAPAVSSMPHEHHL
                     SHSDEYLLMGISTGLVLATILAAFMVYRNYQRKDATGFAKVLENKWYVDELYDRIIVQ
                     PLHWLGGFLKDIFDLKVVDGLVNGAGRLVNYGSRQLRLLQSGQVGSYVLLMVLSMVLI
                     FAIQFFLRK"
     gene            complement(11323..11661)
                     /gene="nuoK"
                     /locus_tag="PLU11_05005"
     CDS             complement(11323..11661)
                     /gene="nuoK"
                     /locus_tag="PLU11_05005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPN58504.1"
                     /translation="MPILNYIILSIALFSIGIVGVLTRRNAIIIFMCIELMLNAVNLL
                     LVAFSKMHMNTAFKAGDLAGTAGTDGQIFVFFIMVVAAAEVSVGLAIIVMMYRNIHSV
                     DVNFLNRLKQ"
     gene            complement(11722..>12078)
                     /locus_tag="PLU11_05010"
     CDS             complement(11722..>12078)
                     /locus_tag="PLU11_05010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPN58505.1"
                     /translation="LNAQFLAIVNIIVYAGAIMVLFLFVIMLMNLNKETEPQKNRWLK
                     IAGALAGGCLMLILVAALKDAAAKEQVASVNQGNIGLIKNLGKELFTTYVVPFEISSV
                     LFLSAMVGAVVIGKKE"
BASE COUNT         3290 a         2984 c         2740 g         3064 t
ORIGIN      
        1 caacaaataa aagaaagata aatcagctaa aagctaacaa taacaggaat ttatgaaaca
       61 aaggaaagat agttactttc cgatacaaaa cttcgaaaaa atataatcca gctgatcctc
      121 attcgtaatc tctccggtga tttcaccgag ataatgaagg catctcctga tatcgagtga
      181 aagcagatcg ccgggtagtt tgttatcgag tccggcttta atttcatgga gagatcctgc
      241 tacctgttga agggcatgat aatgtcgggc attggtgata atggtgtttt cggcctgtac
      301 ctgtccttgc aatacgagat ctaccatccg ttcacgcaat acatcaatat gtcttttttc
      361 tttagcggct atatagacca ccggttcaaa ggcactgaaa cggagacgtg ccgcagcttc
      421 atcaccggcg tcaattttat ttcctacgag taaataacgg agaccggctt catcggcttc
      481 ctttttccag ttggtgagtt cggtgctttc cccgttgaca tcaaaaagat aaagcaccac
      541 atccgcctgt ttgattttgg cgagacttcg ttcgataccg gcggattcaa tcgtatcggt
      601 ggtatgttgt cggatgccgg cggtgtcgat caggcggaaa aggatgccgt cgatattgat
      661 gagctcttca atggtatcgc gtgtggtgcc gggtatctcg cttacaatgg cacggttttc
      721 attcagcagg gtattgagca aggttgattt gccggcattg ggtttgccga caatggctac
      781 agcaaccccg ttacgtatca cattacccag actgaaggat tgaatcaatc cgccggtggc
      841 ggtttgcatt tcattgatca ggtcatagaa ctgggtacgg tcggcaaact ccacatcttc
      901 ctgggaaaaa tccagttcga gttcaatcaa tgcggaaaaa gtaagcagac ggtcacgaag
      961 ctccttcagg ttctcggaga agcctccccg gatggtatga agagcagcct tttgagaagc
     1021 agctgtattc gcagcgatca ggtcagccac cgcttcagcc tgtgtaaggt ccattttgcc
     1081 attcaggaag gcccgttgag tgaactcccc gggtttggcc agcctggcac cggcacggat
     1141 acaggcatca atcacctgtt gttgtacaaa gggggatcca tggcaactga tctctattac
     1201 atcctccccg gtataagaac ggggattccg atacagcgaa atcaccactt catccagtac
     1261 cgtttcaccc tctttgagca aacccacatg cagggtgtga gcagcctgca gcaagagatt
     1321 ttttgaaggg aacagacgat tgaccagttc aatggcccgg gcaccagaga gtcgtattac
     1381 gccaatagca cccaccccgg gaggtgtggc gagggcaatg atggtgtcgt cccagccggt
     1441 cagcttattc atagaggcaa aattagtgga aggggtaagt caaaattcaa aagtaaaaag
     1501 taaaaaatca gccggcagtt ggcagttggc agccagcagt tggcagttgg cagccagcag
     1561 ttggcagttg gcagttggca gttggcagtt ggcagccagc agccaggtta ctaagaaaga
     1621 taaggttcgg gttaaaatat aaaacgcccc tactcactac tcactactca ctactcacca
     1681 ctccccactc cccactcacc actccccagc aactttgtcc tccgaagctc ttcagcattt
     1741 tcaactttaa cgcaattcgg gagcgtagga ggaaaaaaaa tcccccctgt taccaggagg
     1801 gattgcttag aaatattcgt tacagcttat tcagcttcta ctctgaaagt gatcggttgt
     1861 ttgcggtaag cttttacctg gcggccattt tgtacggcag ggctccattt gggtcctttt
     1921 ttaataaccc ggatggcttc ttcttccata ccgtaaccat ggttggtgag ggctcttacg
     1981 tcactgatgt ttccttcttt gtccactaca aactgtacaa cagtcgtgta ggttccttcc
     2041 ggagcaccat tgtcggtggc tacctggcca tttgcgtttc tttcgaggta ctgacgccat
     2101 ttggagtcac cgcccgggaa actggcttcg atttccactt tgtcgaagat tttattttcg
     2161 tcatcttctt tttttacttc gacgatctgc ttaccctggt cgatatccac cggagcggca
     2221 ataccttcat ccttgatacc ttcctggctg accacatcga tcttggcttc tttcagttct
     2281 tcctgcgggg gtggtggttt ttcaacttca tcatcctttt tgatcaccgg aggtgtgaac
     2341 tgtttcattt ccactttcgg aggttcttgc tttggcggag gaggcggagg aggaggcggg
     2401 atggcctttt tctcttcctg cttcacatcc tggatagtca tctcattcat tttcagcttc
     2461 ttgtccgctt tttccttcat tgtgctcgat aagatggagg acaataaagc cagcagggcg
     2521 acggagcctg taatgatgag agcgcgtgta atccggcggt tataagtctt tcttaactca
     2581 tacgcaccat agtctttgtt ctttccgtca aagatgatgt ccagaacatc ggctgagagt
     2641 attttattgg cttccattac gtagcttttt taattagatg cacaagggat tcatttccat
     2701 aaaccccgtt ctgttatttt gcgggagccg ttgcaggcgc cgcaccttca gttaccttaa
     2761 tcagctcaaa ttcctgatca aagattttta ccatggcgta ggtacgcacc tggttgatgg
     2821 tcatttcatc cagcatatcc accgtgtttt tatacgtggc ttcaggactg ggtttaatga
     2881 tcacaacaaa atccttgtcg gcacagttgc tgactggttt tccttccgct ttcgcttttg
     2941 cctcgcatgc cggatcagtc acataccggg aaatcacctc tttctttttg tttacaataa
     3001 tgtcccggat tgaagtgtag gtagccgttt tgaagttgct tccatcttcc ttcagttttc
     3061 cttcataata atagatgaca ttattttttc ccagcatgat gctgagtaca gaggattctt
     3121 ttacctcggt ctgctctttt acatccttca catccttagg catattgagg tcgagcgtcg
     3181 taggggtgct catggttgcc gtgaatacga agaacgtaat cagcaggaac ccgagatcca
     3241 ccattggcgt catatccaca cgggtcgaaa gctttttggc ttttttcacc cctggacctt
     3301 ttttgtggcc accatcgcca ccttcttcta tacttgccat aatgaattgg ttttaaaaat
     3361 tatttcttta aatctttttc accggcggct tccctccggt acaattctgt accagccgga
     3421 atctcatcca gcgcggtaac cagattgtat ttaaactcct cgttcttttt cagggcagaa
     3481 atcaccgctt caaaggttgg atattttgat gctccatccc ctttgatcag gtaattcagt
     3541 ttttcaccac ggaaagcaga ctttgcttca cgaatccacc agaacagctg attgttcagg
     3601 gtgtccagta caggaatacc cggtactttc agctttggct gatcctttgc atccgttgcc
     3661 agtaattgct ttaacccggc aaaaggaaca cctactgcaa aagtcttctt gaagtttgcc
     3721 atttccgaag ggcttaaacc caggttctgg gaggaattga tccctgcaat gattacgtca
     3781 aacttggatt tatctttttc tgatagaatg gtaaagtaaa cccggtctgt cgaatcaatg
     3841 gaaatcagca cggcattgtt ttcaggcagt ttttgtgaca aaactgatcc cggagtagtg
     3901 atctctaccg gctcctgcgg tttgaacttg gtcgccataa tgaaaaagga caggataagg
     3961 aatgccacat ccacgaaggg tgtcatatcc gtaaacgtac tctttttagg tatcttaaca
     4021 cttggcatga aacttaattt agtaattaga tgaaatagta ttcattttcc atatcaaaac
     4081 cgcgcttctt atttatacag ggaagcgaag ctttgagtca gggtgaaacc tgattcatca
     4141 atgccataag tgatagagtc gatcttggtt gtgaagatgt tatacatgat cagggccagg
     4201 gcagatgtac cgataccgag ggcggtatta tacagggcct cagagatacc gatcgccagt
     4261 tcaccggctg cactaccacc accttcatta ccgagggctg agaatgattt gatcataccg
     4321 ataaccgttc ctaagagggc gatcagggta ccaacggatg tgatcgttga aaggaatacc
     4381 aggttcttct gcagcatcgg cagttccagg gcggtggctt cttccacttc tttctggatc
     4441 gcgattactt tctgctccgt attcaggttg ccttcagaaa tcatttcttt ataacggcgg
     4501 agacctgctt tcattacatt tcctacagaa ccacgctgtt tgtcgcactc tgccagggca
     4561 gcgtccacat tcttattggc caggtgatat tgtactttgc ggataaaatc agcaatagaa
     4621 ccattaccga gagccttacg gattgtcaac agacgctcaa tggaaaacac gataaccatc
     4681 aggagcatac cgatcagcag gggaacgata ataccacctt cataaatacg gctgaatgcg
     4741 tccttgggac ctttgtggct gggccagaat ccaccggcag gatccggctg ttcaaatgaa
     4801 gcggcattac ccatcataaa acgccagatt gcataaccgg cgagcacaca aacaataggt
     4861 gcgatccatg aaatggcgtt gctgcttttc tttgcctgaa ctgtggttgc cttacctgat
     4921 gctgttgcag ttggtttaat ctctgccatg atctttgttt tttagttttt aaaagttaga
     4981 atagtcatct ctttcagaga cacaatgcta agaaaaaagt tgataaaaaa atccgccatc
     5041 ccggcttttt cgggaagggg cttcaagtta aggatttttt aatacggaac catttcctgc
     5101 taaattattt acaggaaatt catagagaaa acgtttgcga tataggtcct gaagggatta
     5161 ttcataccgg agcgcttcga tgggattgag gcgacccgct tttaaagcgg gatagagacc
     5221 ggccagcaaa cctaccaggg tacagatcac gatgccgtaa atcacccagt tccagggcac
     5281 cacaaagccg gtattgagta ccagcgaaaa gctgttgccg accaatattc ccaggataat
     5341 cccgaaaatg gcccccagca tgctgattat aatggcttcc agtaaaaact gacggctgac
     5401 catctggctt ttccctccga ttgccttgac caggcctact tcccgggtcc gctccgaaac
     5461 agataccagc attatattca tcaggccaat ggcagcaccg atcagggtga tcagcccgat
     5521 taccgtggcc gaaatagtaa ggaaacgaag actgttcatg gctttttccg ccacactgtc
     5581 acttcggtcg agcacaaaat tactctcctc tgtaatcatg agtttgcgga tcgccctgaa
     5641 tgtaccttcg gcttctccca ttgcggcatt gaccttgatc aggtcattgg cctttacggc
     5701 aatcacatat gaaaagccgg atggaaaatt gcggtctacg ttggtatatg tagtaatgat
     5761 aatattatcc cggttaaacc caaaagaaga gcccttactc tccaatacgc ccagcacacg
     5821 gaacggaata ttgttgatgc gcaccacccc gtttaccgca cctgcaggcc cttgtttgaa
     5881 caagcgtttg gccacatcat aacccagaat acaaacatta cggccggtct gcacatccat
     5941 ccggttcata ttgcgtccgt attgcagact gaatccattc aatgcaaggt aattctcatc
     6001 cccgccaaac atcaggacat tgggactggt cttgaagttc tcatgggaaa caatattatt
     6061 ccggttggaa aaaatggaga tgcttttcat tgagggaaaa tcaaaacgcc gtacaaaaag
     6121 ctcagcttcg tccttggtga tatttttatc gagattggat acccgggatt tacgaccgcc
     6181 acgggtactc agcttcattt cactccggtt gcctccgcca aaacggatat tccgttcttt
     6241 gaaacggatg gtaaacgcat tcgcccccat ggaagaaaag ctttctgtaa acttctggtt
     6301 catcgcctgt atagccgtga taatacctat cagtgccata atcccgaatg cgataatgga
     6361 aacggtaagt cctgtcctca gtttattacc tcggacggtg cgaaaagcca gggagaatac
     6421 atcacggaaa ttcatatatg ataatcgcgc agatcggttg tctgttcccg ctcctcaaag
     6481 atagttaatg cccggatgga agcccggtta aaagtacagg cggcctaaaa gcagttcagg
     6541 ataaaccccc agcaggataa ccaggaatgc caacaccagc aacatccctt tgaaaaagga
     6601 gggaaatgcc ggatgcgggg tgtcgccggg ttctttaaaa tacatcgcct gaatcaccct
     6661 gaaataataa taaacgctga ctgccgccat taatacggca aagatcacca gccacaaact
     6721 tccgggggta cccagggcgg ctttcagcat ataaaattta gccatgaagc cggctgtcag
     6781 tggaatacct gcaagggata ataaaaatac agtaagggtg gctgccacca tgggttgtga
     6841 tttggcaagg ccattaaatc cgtcaaatgt ataatcgctc atgcgcgcca gcacggcaaa
     6901 gatgccgatc gtggccgcac tgtaagctac tgtataaagc atcaaccctt ctttcccatc
     6961 cccattcatt gcatataaag caaacaacat aaaaccggcc tgggcaatac tggaataagc
     7021 cagcatccgc tttacactct gctggaaaac ggctgttata ttaccgataa acaatgtcac
     7081 cgccgtcatg accgtcaccc agagtctcca ttgaccctgt tggggctcaa acatatcatc
     7141 gaatagccgg ataaagccga agaaaaccac cactttgacg attgttgcca tgaaggaagt
     7201 aaaaacagtt ggagccccgt cgtacacatc cggtgtccag aaatggaaag gcgccgccga
     7261 tactttaaaa gccatggcaa aaaggaggag gatcataccc gtgtaaagca aactcatatt
     7321 ctgtggtatg atggccggac tgataacagt actgaacgaa gccctggctc cgtaaagcag
     7381 ggcgatcccc atcagcatga gaccggttga aaaggaaccc agcaaaaaat atttcagggc
     7441 ggcttcattg cctttcaggt tgcgtttatc actgccggta agtatataca gcgggatgga
     7501 aataatttca atccccagga ataaaataag cagggatttg aatgaagtta ccagtgtggc
     7561 cccgcaaaga ataaagaaga tcagggcaaa atattccgca taattcacgc ccaccttctc
     7621 catatccgga gccgataata aaagataaag cagcaccacg gcaaagatga ccgtattaaa
     7681 gaccagggcg aaatgatcaa agacgatcat ctcccgggta tcaatggaga aaagctgaat
     7741 ccctttcagg tcaagaatac tcatacccag cagcaccagg atgccggcaa tggccactgt
     7801 acggatagcc gatttttgct tcagcagaat accactgaac atcatcacca ctcccagtac
     7861 agccgaaatt attaatgcgt tcatacgatc gtttccttat tacttgcgga ataaataact
     7921 aacatctatt ttactaaacc attctttgga gaaactgtcc gtcagctcca gcatggactg
     7981 cggatagatc cccagccaaa ggatgatcac cacaatcagc cccagcgcca gttgctcatt
     8041 cagtttcaga tccgttaccg gcaccctcag gctaacggct tccccgtaaa atgttttacg
     8101 gatcatattc aatgtgtaca cagcggccag gataataccc agtccggcga gtactgtaaa
     8161 gacaacttta aagttggatt gtgccgacaa caacccgtta aacataagga attcaccggc
     8221 aaatccgttg gtcagcggca gtgctatatt cgccagggca atgaccacaa agaagatggc
     8281 cataaccggt gcctgctggg cgagtccgcc taattcagat attttccgtg tgccatatct
     8341 gcgttcaata atctccacga taatccagag gccaatgata ttaactccgt gattgaatag
     8401 ttgaatcatc acgccctgtg tgccactgag gttttcagca aacaaagcag ccgccatcag
     8461 gctgatatgt gcaatggagg agtatgcgat aagtcgtttc aggtcatcct gacggatggc
     8521 caccagcgag gcataaatga ttccgatcac tgcgaggatc atcaccacat caccccattg
     8581 gaaagaagcc agcggtaata ccggcatcag ccagcggatc aggccgagca ggcccatttt
     8641 aaccatgacc ccgctgagga tcatcgtggt tgccgtaggc gcctgttcat atgtatccgg
     8701 ctgccaggtg tggaagggaa agaggggcat cttgatcgca aatgccacaa agaacagcca
     8761 gaataaccat agctgatcac tggccggaat cttcagtttg taaaatgcac tgagtccaaa
     8821 tgaatgatcc ggcgtctgtg attgcaggta taaaataccg atcagcatca gcaaggagcc
     8881 caggaaggta tacacaaaga atttaaaggt gactgcgatc cgcttttccc caccccactg
     8941 ggaggccaga aaatacatcg gaattaatgc cagttcccag cagaaataaa ataacaaggc
     9001 gtcattggcc aggaacacgc ccatcatacc ggcctgcgcc agcagcatga gtccaaaaaa
     9061 gcgattggca tttttcgggg atgcctgcca ggtacccagg aaggtaagcg gatacgcaat
     9121 agcggtcagc aggcagagta cctgtcccat cccgtcaatc ttcagcgcaa aactgctgcc
     9181 cagcgaactc atccatgggg cgctgaattg cagattgctt tcggaattca tcagggtcag
     9241 gcccagtaag gagacagcca acgtggccaa tgaggctaaa aaagcaaatg cctttgccgt
     9301 cttttcttcc cggatgagga agctaagcaa ccctgtggcc aggggaatca gtatcagtaa
     9361 gaatgcgatc atatcaatag cccgcctttg gcggtggatt tattttctca gaaaaaattg
     9421 aattgcaaaa atcaacacca tactcagcac catcagtaat acataactgc ctacctgtcc
     9481 gctctgcagg agcctcaatt gacggctgcc gtagtttacc aggcggccgg ctccgttcac
     9541 taacccatcc accactttca ggtcgaagat atctttcagg aacccaccta accaatgcag
     9601 gggttgtaca atgatgcggt cgtataattc atccacgtac cacttgttct cgagtacttt
     9661 ggcgaatccg gtggcgtctt tccgctggta attccggtaa accataaaag ccgcaagaat
     9721 tgttgccaat acaagtccgg tggaaatacc catcagcagg tattcgtcac tgtgtgataa
     9781 atgatgttcg tgtggcatgg aacttaccgc cggggccagg aaagcactca gtttatggcc
     9841 tccctcacgg atcacttccg ggatgccaac cagtccgcca acaatggata acactgccag
     9901 tatgatcaac ggcaatgtca tggccagggg actttcatgc aggtgatgcg cctgctcatc
     9961 tgtacccctg aatttgccca taaacgtgat tataagcaaa cggaacatat agaacgcggt
    10021 cagtaaggcg gtaaatacgg ctactgcata caaaaccgga ctcttcataa aagcacttaa
    10081 caggatggcg tctttagaga agaagccgct gaatggagga ataccggcaa tcgcaataca
    10141 cccgataaag aaagttacat aggtgatcct gagtttcttg ctgagaccgc ccatattccg
    10201 gatatcctgt tccccgccca ttgcatggat aacagatcca ctacccagga ataacagtgc
    10261 tttgaaaaaa gcatgggtca ttacatggaa tacagctgcc acatattcac cggtacccag
    10321 ggccacgaac ataaagccca gctggctaac ggtggaataa gccaatactt ttttaatatc
    10381 gttttgtttc aacgcaatgg tagcggccag taaagcggtt gccattccgg tataggtgat
    10441 gatattgcgt gtcagttcgg ccgcatccat actatacaac acatgactac gggctatcat
    10501 ataaatacca gccgtgacca tggtagccgc gtggatcagg gcggacactg gtgtgggacc
    10561 cgccatggca tccggcaacc aggtataaag cggaatctgt gcacttttac ccgtagcccc
    10621 gataaagagt aacaacgtaa tcacggtaat atcggaagcg ctgagttttg ccaggttaac
    10681 agctgtaaac aattcatcaa aagaaacagt acctaccctt gcaatcagcc agaagatcgc
    10741 gatcaggaac gccaggtccc cgatccggtt catgataaaa gctttattgg ccgctttatt
    10801 atactcctgt ttcttgaacc aatagccgat cagcaggtat gagcagagtc ctacaccctc
    10861 ccacccgata aacaagatca ccaggttggc gcccatgacc agcagcaaca tggagaaaac
    10921 aaagagattg aggtaagcaa aatagcgtcc gaaatggccg ggcttctctt cgtgcatata
    10981 ggaagccgaa tacacatgaa tcaggaatcc gacgccggta atgatcagca ggaaaacagc
    11041 agaaagctgg tcgatcttta acgcaaaggg aattaccagt ttgtctactg cgataaaatt
    11101 aaaatactcg gcaacaaacg tattgccgtc tttcacctgc atgaataccc agatgctgag
    11161 gatgagtgaa ccgagaatac tacccgagcc aatcaggccg atcagtgttt tggataaata
    11221 gtttctgccc agcccgttga tcaggaagcc cagcaagggc agcaggggaa tcaggtaaac
    11281 tatctgtaaa gcgttattca tatactattt cccgtccggg gcctactgtt ttaatcggtt
    11341 taaaaaattc acatccaccg aatgtatatt gcggtacatc atcactataa tggccagtcc
    11401 cacgctgact tccgctgccg ccaccaccat gataaaaaat acaaagatct gtccgtcggt
    11461 accggctgtt ccggccaggt cacccgcttt gaaagccgtg ttcatatgca ttttggaaaa
    11521 ggccacgagg agcaggttca cggcattcag catgagttct atgcacatga aaatgatgat
    11581 cgcattgcgg cgggtcagta cccctacaat ccctatactg aacagggcaa ttgacaggat
    11641 gatataatta agtatcggca tatcgtacgt ttagaaggct ggtgaaccgt agttcaatac
    11701 cttcctggtt agttaaataa tttactcttt cttcccgatc accacggctc ccaccatcgc
    11761 actcagaaac aaaacactgc tgatttcaaa cggtaccaca tacgtggtaa agagttcttt
    11821 tccgaggttt ttgatcaacc cgatgtttcc ctgattcact gacgcaacct gttcctttgc
    11881 tgccgcatct tttaacgcag ccaccaggat cagcatcaga cagccgccag ccaaagcgcc
    11941 ggcaattttc agccagcggt tcttttgtgg ttctgtttcc ttattcaggt tcatcaacat
    12001 gatcacaaac aggaacaata ccatgatcgc cccggcataa acaataatgt tcacgatcgc
    12061 caggaactgg gcattgag
//
LOCUS       DAOPMW010000061        12038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5552_length_12038_cov_11.693316, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000061 DAOPMW010000000
VERSION     DAOPMW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5552_length_12038_cov_11.693316"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..2010)
                     /locus_tag="PLU11_05015"
     CDS             complement(241..2010)
                     /locus_tag="PLU11_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58506.1"
                     /translation="MKKTTISILTLALLFTAGLSAQTLQDGKNHLFAERYNSAINSFN
                     QLIASNPNNIEAIYWLGQSILQSEEIMSSRIAKTRQLYEKAMQTSNGAPLITVGLGHV
                     DLLENKTNDARQKFETALTMTRTKKGENIDILNAVGLANADAKAGDYEYVIAKLKDAI
                     DNKNAKNPETAVWLGNALRKARPGEGGGEAFRYYTKALEFNPNFPLANLRLAKLFESQ
                     KNWELVFQYLTAATKNDDKFTAAYYELFYYYFVRLDYTSAEAQLKKYIDSKMPEKDIQ
                     DEYLYAMLCWANKDYNCATSKGEVVVKSLGENTKPKVYRLLADAYFTKTEFSEAKKYS
                     DAFFNKKNPDDLLLYDYQLRADILDKTGGTMDEVFNTYLQGALLDTTASLKVDFLKTG
                     ADKFKAKGDSASREREGDLRLQMIRIKGTPSQRDYFDAGFAYYQAKNYHRADSVFDVF
                     VEKFPEETYGYMMEYNIHRAIDSTMERGDAVPWAEKYLQILEKDTVKNKNSILGVAGY
                     LAQYHANIAKDKEKAIEYLQRMLNLDPTNEVIQKNIEILKKSLSPDTRPASNQPGKST
                     APVAKTVKTPPGAKTAATPVVKK"
     gene            complement(2052..2993)
                     /locus_tag="PLU11_05020"
     CDS             complement(2052..2993)
                     /locus_tag="PLU11_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPN58507.1"
                     /translation="MNGTGMRCIANKAAFFLLFIVMGLFSCKSFEEQERALPDTSDRG
                     TIYVSADESFKPVIDEQVQVYESNHPGTKINVEYKPEADCLKDLLTDSVRMVIVTRRA
                     SVDEKEVVADSLKVQARSMVVARDGVAVVLPPGSPDTLLTMNDIRAILTGKFKKNLIP
                     VFDGVKATSTVRFIIDSILKGDSLTPAAMAARSSEEVIDYVSENSGVVGFIGVSWIGN
                     REDSSYLSFSKKVSVAYLQATDIPDSYVKAYQANIYLKRYPMVRDLVYILKENHRGLG
                     TGFANFMSGEIGQLIFKRAYLAPSFKFLSIRPVKLNE"
     gene            complement(2990..3241)
                     /locus_tag="PLU11_05025"
     CDS             complement(2990..3241)
                     /locus_tag="PLU11_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58508.1"
                     /translation="MLEEYERRKRKQVAGMKSLMDYGMGGLILLMGLVFIFRMYLGVN
                     HPVNIKMGKPDTLEKIFGGLSIVYGLWRIYRGYKKNYFR"
     gene            complement(3330..4145)
                     /locus_tag="PLU11_05030"
     CDS             complement(3330..4145)
                     /locus_tag="PLU11_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPN58509.1"
                     /translation="MKENNTPSLSFDDIVFENRNKSYGAYDLRNGYANRMLAALGAGM
                     SVLLLGTLFFINRDNTAEEMPVVVRGEGLLVRTIEIPIEKPVETLKPNEARPQKQVQH
                     TATVKYISNIKIEKEVKEEMVAVEDIAGKNIGDKDQKGEKADGTVGPEPETVADGNGT
                     GLSAPAAPDFSAIEKAPEFPGGAAALRKFLARNLVSPGNLEEGEMKTVRVRFRVEKDG
                     SVSSLEILSTAGDDFDDEVVRVCRRMPKWIPALQNGTNVPVSYILPVTFMGSE"
     gene            4290..4754
                     /locus_tag="PLU11_05035"
     CDS             4290..4754
                     /locus_tag="PLU11_05035"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPN58510.1"
                     /translation="MKILMVCLGNICRSPLAEGILQDKAFKAGLNWSVESAGTNGYHT
                     GEAPHPLSQKVARINGIDISQQRSRRFRAEDFQVYDKIYALAGDVMEEIKFIARKSFD
                     PLKADLLMNELHPGKNKEVPDPWYGPEPGYHEVYRMIEEVCEEIVRKYGTKI"
     gene            4818..6206
                     /gene="hisS"
                     /locus_tag="PLU11_05040"
     CDS             4818..6206
                     /gene="hisS"
                     /locus_tag="PLU11_05040"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPN58511.1"
                     /translation="MAKASIPQGTRDFGPDVVRKRMYILNTIRSVFELYGFQPLETPA
                     MENLDTLMGKYGEEGDKLIFKILNNGLDNPEKETQVREDFEKVLQGKNNKGITERALK
                     YDLTIPFARYVAMNHAQLVFPFKRYQVQPVWRADRPQKGRYREFYQCDADVVGSQSLL
                     NEVELASIYHEVFTKLGLTGYELRINSRKVLAALAETCGGADKMTDITVAIDKLDKIG
                     IDKVKEELATRGLTDDQINIIEKYLNITGTNDEIIAGLSQLIGGNLNGQKGIEELQLL
                     TNAFKDADSRLPTPDSRLFVDPTLARGLNYYTGIIFEAKAPATVKMGSIGGGGRYDDL
                     TGLFGVPNIPGVGVSFGVDRIYDVLEELKLFPADVHVGTQVLFFNLGEAESKKAFELL
                     QQLRGQGIRGELYHESSKFDKQFKYAEKKNIPYIVILGEKELASNSCNLKELATGVQQ
                     TLSFTDLLNYKF"
     gene            6209..8011
                     /locus_tag="PLU11_05045"
     CDS             6209..8011
                     /locus_tag="PLU11_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58512.1"
                     /translation="MKIKHLILIALVLISSQLSFAQDNLLYKYIQPGSRMVMRFNLIK
                     IASKVPGETFRASSLYQDMMKNDNGEIKNFLSDPSGTGIDFSTDFILTVITGEDGEST
                     SPLLIARLGDSGKFTALMEKMNKGEVSRIQVFGSNRLLLPKSFGPAIAWNDEVMIVTG
                     ISNGKKELNAVFIDTSDTRDMDVRMNEVAERLNNELKEKCFAALTPNTNNTLVNSPVF
                     SKLMNESGDIRIWNSGVKPATTKEIQQLPPFVTRFLSTLQAAQGTESTSIVNFENGKI
                     AGAIRNYISPEMGSIYQKYPQEPLPTQLVSRLPQGKILMLMMSAFNKDMSGEISKQNG
                     MAGFIDSLKQYLKVDLSLFQKGFQNKAMMAIMEMPAKEDDNGEKKKILENLGLFIVIP
                     VADKAAVAQLKALADRKMDSLAATEKGEKIMSVFRPAIMYNDSLCVIAHSAAMAEAYL
                     NHSGNAPLPAWLPSNQKGNMWMSLGFRDIMTMVFSMAGKPGKSKNNQTMEMLEMFDQL
                     VITGGDYAEGSMNSQMEFRFSNPDKNVLEQLFEMANRASMQKAKAKKEMADTPEIMQD
                     DVVTDAPKEAPPAPKKAPVKKPVSKSKTPAKTKG"
     gene            complement(8089..8463)
                     /locus_tag="PLU11_05050"
     CDS             complement(8089..8463)
                     /locus_tag="PLU11_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58513.1"
                     /translation="MKKIVLALFAFLAYTAAQAQTDTLFQQYTGKYIFPDGSPVKEIG
                     VIVQEGVLTATSVMGNSELRKTDNKDVFELVVYAGTATFKRNADGKVNGLQIQVEDVN
                     MEGTKTEEGIGYFLRKREVVQW"
     gene            complement(8529..9722)
                     /locus_tag="PLU11_05055"
     CDS             complement(8529..9722)
                     /locus_tag="PLU11_05055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPN58514.1"
                     /translation="MNPVYIIDTVRTPVGRYGGILSSIRPDDLLAYIIRALLRRNETV
                     DPAAIEDVIAGAANQAGEDNRNVARMAALLAGLPVTVGGNTVNRLCASGLQAIMDGAR
                     QIACGDAELVIAAGVESMSRAPFVQPKATEAFGRTAEIYDTTMGWRFINKTLASIYHP
                     FTMGETAENVASQWKISREEQDVFALGSQQKYAAAKAADKWADEITPVELTQKGLVSW
                     VVNDEHPRETSLEKLAALKPAFLKEGTVTAGNSSGINDGSAAMLLASEGAVKKYGLQP
                     MARIRSMATAGVDPAIMGIGPVPATQKALQRAGLSMGKMDLVELNEAFASQSLACMRE
                     LELDPEKVNVNGGAIAIGHPLGCSGVRISATLLHELWRRNARFGLATMCVGVGQGAAI
                     IYEKC"
     gene            9791..10834
                     /gene="rlmN"
                     /locus_tag="PLU11_05060"
     CDS             9791..10834
                     /gene="rlmN"
                     /locus_tag="PLU11_05060"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPN58515.1"
                     /translation="MKKTQKNIRHLTLAEIETFFAELGEKKFRAKQVYEWLWQKGALS
                     FADMTNLSKDMRQLLGEQFTFPSLTIDATQISEDGTIKSRFKTWDGHLVEGVLIPTTA
                     RYTACVSSQIGCSLSCKFCATGYIDRKRNLEFDEIYDEVVLINQQSERVHGKKLSNIV
                     FMGMGEPLLNYKNVIRSIERITSPDGLAMSPRRITVSTAGVAKGIRQLGDDKVKFKLA
                     LSLHAATDAKRHEIMPINDTNNLKTLTDALNYFYKQTGNEITFEYILFKDFNDSLKDA
                     DDLIKIYRQVPADLINIIEYNPIEFAKYEKPEEAKVQAFMQYLEKHRVNVRLRKSRGK
                     DIDAACGQLANKG"
     gene            11095..11808
                     /locus_tag="PLU11_05065"
     CDS             11095..11808
                     /locus_tag="PLU11_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58516.1"
                     /translation="MKKLMLPVLAIALVFSANAQEIPERKADHPPIMHHKQKGGPERH
                     KAMKELNLTDAQKEQFKTEKDAFRKKMEELKKNDNITVKEWKAKKESLVKEHKAKMES
                     VFTPEQKAKMEQRKKEGQAKQQDMMKQRAEKMKTQLGLTEEQSAKLEKSRKEIGDKMK
                     AIRENKSLTEEQKREQMRELGKKQKENMKSILTEEQLKKMKEGKKQGPGRGEGPGKGP
                     KGPKGHKTPPPPPVEKETL"
BASE COUNT         3412 a         2883 c         2646 g         3097 t
ORIGIN      
        1 gccataagaa aggcaactaa tcctacggca aatgcaatct gtagggctac cggcagataa
       61 ctcgctgcat ttgcattagt tgaaccggtt tgaagcaggt aaatcataaa cttttttact
      121 ccgccgcaaa aataaggcgg ctgtctgaat ttacctgaat taaaaatcag gcaggcccct
      181 gatacaaaag aaaagccccc gcaaatgcgg gggacttttg taaaagaaga aaaaccgagt
      241 ttatttcttt actaccgggg ttgctgctgt tttagcaccg ggtggcgttt tgactgtttt
      301 tgcgaccgga gccgtgcttt ttccaggctg gttactggcc ggacgggtat ccggagaaag
      361 cgatttcttc aggatctcaa tatttttctg aatcacttca tttgtggggt ccaggttcaa
      421 catccgttga agatattcaa tggctttttc cttatcctta gcaatattcg catggtactg
      481 agccagatat ccggctaccc cgagaatgct gttcttattt ttgacggtat ctttttccag
      541 tatttgcagg tatttttcag cccagggcac ggcatcaccg cgctccatgg tgctgtcaat
      601 agcccggtgg atattatatt ccatcatata accatatgtc tcctctggga atttttccac
      661 aaacacatca aaaacactat ctgcccgatg gtaattcttg gcctggtaat aagcaaatcc
      721 ggcatcaaaa taatcacgtt gactgggtgt tcccttaatg cggatcattt gcaggcggag
      781 atccccttcc cgctcacggc ttgcactatc cccttttgcc ttgaacttat cggctcctgt
      841 tttcaggaaa tcgactttca gcgaggcggt ggtatccaat aatgcccctt gtaaatatgt
      901 attaaagact tcatccattg taccacctgt cttatccagg atatccgctc tcagctggta
      961 atcatacagc agcaagtcat ccggattttt cttgttgaag aaagcgtcac tgtatttttt
     1021 ggcttctgaa aattctgttt tggtaaaata ggcatcagcc agcaaccggt atacctttgg
     1081 tttagtattc tcacccaggg acttcaccac cacttctcct ttgctggtcg cacaattata
     1141 atctttattt gcccagcata acatggcata caggtattca tcctgaatgt ctttctcggg
     1201 cattttcgaa tcgatatatt tcttcagctg ggcttcagcg gaagtataat caagccggac
     1261 aaagtaatag taaaacaatt cgtaatacgc ggcagtgaat ttgtcgtcat tttttgtagc
     1321 ggctgtcagg tattgaaaca ccaattccca gtttttctga gactcgaaca gtttagccag
     1381 ccgcagattt gccagcggga aattcggatt gaattccagg gcctttgtat agtaacggaa
     1441 ggcttctcct ccaccttcgc cgggacgggc tttacgaagg gcattaccca gccatacagc
     1501 agtctccgga ttttttgcat tcttgttatc aatcgcatct ttaagtttag cgatcacgta
     1561 ttcgtaatcc cccgcctttg catctgcatt ggccagtccc acagcattga gaatgtcgat
     1621 attctcccct ttcttggtcc tggtcattgt aagtgcggtt tcaaatttct ggcgggcatc
     1681 attcgtctta ttttccaata aatccacatg acccaatccg acggtaatca ggggtgcccc
     1741 gttactggtc tgcatggctt tctcatacag ctgacgggtt ttagcaatcc gggaagacat
     1801 gatctcctca ctttgcagaa tggactgccc cagccagtag atggcttcaa tattgttcgg
     1861 atttgaagca atcagctgat taaagctatt aatcgcacta ttgtaccgtt ctgcaaaaag
     1921 atgattttta ccatcctgca gcgtctgggc gctgagacct gccgtaaata ataaagcaag
     1981 tgtcagaatt gagattgtgg ttttcttcat tttactgttc ccttttaaaa gttgtaaata
     2041 taaatcattt gtcactcatt taactttacc ggtcttatcg agagaaattt aaagctgggt
     2101 gccaggtaag ctcttttaaa aataagctga ccaatctccc cgctcataaa attcgcaaag
     2161 cctgttccca atccccggtg gttctccttc aggatataaa ccaaatcccg taccatcgga
     2221 taccttttca ggtaaatatt tgcctgatac gctttcacat acgaatcagg aatatcagta
     2281 gcctgcaggt aagcaacgct gactttcttc gaaaaactca aataactgct gtcttccctg
     2341 tttccgatcc agctcacccc aataaaaccc accactccgg aattctccga tacataatca
     2401 attacttcct cactgcttct ggctgccata gccgccgggg tcagtgaatc ccctttcaat
     2461 atagaatcga tgataaaacg taccgtactg gttgctttta ccccgtcaaa taccggaatt
     2521 aaatttttct taaattttcc ggtcagtatg gcgcggatgt cattcatagt caaaagtgta
     2581 tccggtgagc ccgggggcag gacgacagct actccgtcac gggctaccac catactcctg
     2641 gcctgtactt tcaacgaatc tgccaccact tctttttcat ccaccgaagc tctcctggtc
     2701 acgatcacca tgcgaacact gtctgtcagc aaatccttca ggcaatctgc ttccggttta
     2761 tattccacat tgatcttagt tcccggatga ttggattcat atacctgtac ctgttcatcg
     2821 atcaccggtt tgaagctttc atccgcactc acataaatag ttccccggtc actggtgtcc
     2881 ggcaaagctc tttcctgctc ctcaaatgat ttacaggaaa acaaacccat gacaataaag
     2941 agtaaaaaaa aggctgcctt attcgcgata caccgcatac cagtaccatt catctgaaat
     3001 aatttttctt gtatcccctg taaatccgcc ataacccata gactatagaa aggcctccga
     3061 atattttttc aagtgtatcc ggttttccca ttttaatatt aaccggatga ttgacaccta
     3121 agtacatcct gaaaataaag accaggccca tcagcaggat cagtcctccc attccataat
     3181 ccatcaggga cttcattccg gctacctgtt ttcttttacg tctttcgtat tcttccaaca
     3241 tggaatcaca tttattaatc cctgccttaa ccatttttgc cattagaaaa ccaggcaact
     3301 ccagggtgga gcaaattaga taaattttgt tactctgaac ccataaaagt tacgggcaat
     3361 atatagctta ccggcacatt tgttccattt tgcagggccg gaatccattt gggcatcctg
     3421 cggcataccc taactacttc atcatcaaaa tcatcacccg ctgtggacag tatttcgagg
     3481 ctgctgacag atccgtcctt ttcaaccctg aaccttacac gaacggtttt catctcacct
     3541 tcttccaggt tgcccggtga taccaggtta cgggccagaa acttccggag tgcggctgct
     3601 ccacctggaa attcaggtgc tttttcgata gcagagaaat ccggagcggc gggtgctgac
     3661 aatcctgtcc cattaccatc agccacagtt tcaggctcag gtccgacagt tccatccgcc
     3721 ttttctcctt tctgatcttt gtcccctatg tttttcccgg ctatatcttc caccgctacc
     3781 atctcctctt tcacttcctt ttcaattttt atattactga tatattttac ggttgcggta
     3841 tgctgcacct gtttttgcgg acgggcttcg tttggtttta gcgtttccac cggtttttca
     3901 attggtattt caatcgtgcg gacaagcaat ccctctcccc gaacaaccac cggcatctcc
     3961 tcagccgtat tatccctgtt tataaaaaaa agagtaccca acagtagtac agacattccg
     4021 gctcccaatg cggccagcat ccggttggca tacccgttcc gtaaatcata ggctccataa
     4081 ctcttattac ggttttcaaa gacgatatcg tcaaagctca gtgatggggt gttgttctct
     4141 ttcataattg ctgctttgac agtaagatgc agcaattaca taaatccaca cggattaccg
     4201 gtcttagcaa aaaccttaac aatccattca atgtgtatta ttcggatgca aatcatgtat
     4261 agctccctcc ggctgtttat atttgcagca tgaagattct gatggtctgc ctggggaata
     4321 tctgccgcag tccgctggca gaaggaattc tgcaggacaa ggcctttaaa gcaggcttaa
     4381 actggagtgt ggaaagtgcc ggtaccaatg gctatcatac cggcgaagct ccgcatccat
     4441 tatcacagaa agtagcccgg atcaatggga tcgatatcag ccagcagcgg tcgcggcggt
     4501 tccgggcgga ggacttccag gtttatgaca aaatctatgc gcttgccgga gatgtgatgg
     4561 aagaaatcaa attcatcgcc cgtaaatcgt tcgatccgtt aaaggccgat ctgctgatga
     4621 atgaactgca tccgggaaaa aacaaagagg tgccggatcc ctggtacgga ccagaaccgg
     4681 gctatcatga agtataccgg atgatcgagg aagtatgtga ggagattgta cggaaatacg
     4741 gaacaaaaat ataatgcagc attttcccgt gaacacaata ttcaatactc aatattcaat
     4801 tttcattacg caataaaatg gcgaaagcaa gcatacccca gggaacacgt gatttcggac
     4861 cggatgtggt acggaagcgg atgtatatcc tgaacacgat ccgatcggtt tttgaattat
     4921 acggttttca gccgctggaa acaccggcca tggaaaacct ggataccctg atggggaaat
     4981 acggggaaga aggcgataaa ctgattttta aaatccttaa taacggtctc gataatcctg
     5041 aaaaggaaac ccaggtccgg gaggattttg aaaaagtatt acaggggaaa aataataaag
     5101 gcattaccga gcgggcgttg aaatatgacc tgacgattcc atttgcccgg tatgtggcca
     5161 tgaatcatgc gcagctggtt tttcctttta aacgttacca ggtacagccg gtatggcggg
     5221 ccgatcgtcc gcaaaaaggc cgctaccgcg aattttacca atgtgatgcc gatgtggtag
     5281 gcagtcagtc cctgctgaac gaagtggaac tggcgtccat ttaccatgaa gtatttacca
     5341 aacttggatt aaccggatat gaacttcgga tcaacagccg caaagtactg gccgcattgg
     5401 ctgaaacctg cggaggtgcg gataaaatga ccgatatcac cgtagctatt gataaactcg
     5461 ataagatcgg tattgataag gtaaaagaag aattagccac ccgcggttta accgacgacc
     5521 agatcaatat cattgaaaaa tatttaaaca ttaccggaac aaatgatgaa atcattgccg
     5581 gactatcgca gctgatcgga ggaaacctca atggacaaaa aggaattgag gagcttcaac
     5641 tattgacaaa tgcgtttaaa gatgctgact cccgactccc gactcccgac tcccgactat
     5701 ttgtagatcc aacacttgcc cgcggcctca actactatac cggcatcatc ttcgaagcca
     5761 aggcccctgc cactgtaaaa atgggcagta taggcggcgg cggacgttat gatgacctga
     5821 ccggtttatt tggtgtaccc aatatacctg gtgtaggtgt ttctttcggt gtagaccgta
     5881 tttatgatgt gctggaagaa ctgaaactgt tcccggcaga tgtacatgtc ggaacacaag
     5941 tgctgttttt caatctcggt gaagcagaaa gtaaaaaagc ctttgaactg ctgcaacaac
     6001 tccgcggtca gggtatccgt ggggaactct atcacgagtc atccaaattt gataaacaat
     6061 tcaaatacgc agagaaaaag aatattcctt atatcgttat cctcggcgaa aaagaactgg
     6121 ccagcaacag ttgtaacctg aaagaactgg ccaccggggt gcaacaaacc ctttctttca
     6181 ctgatttact gaactacaaa ttctgattat gaaaatcaaa caccttatcc tcattgcgct
     6241 ggtgctgatc agcagccagc tgagttttgc acaagacaat ctgctttaca agtacattca
     6301 gcccggcagc cgtatggtga tgcggtttaa cctgataaaa atcgcctcca aagttcccgg
     6361 cgaaactttc cgtgcgagtt cgctttacca ggatatgatg aaaaatgaca atggagagat
     6421 caaaaatttc ctgtcagatc cttccggaac aggcattgat ttttcgacgg actttatcct
     6481 gacggtcatt acgggtgaag atggagaaag tacatcacca ctgctgatcg cccgccttgg
     6541 tgattccgga aaatttactg ccctgatgga aaaaatgaat aagggcgaag tcagccggat
     6601 ccaggtattt ggcagcaacc ggctgctatt gcccaaatca tttggccctg ccattgcctg
     6661 gaacgatgaa gtaatgatcg taaccgggat cagtaatggc aaaaaagaat tgaatgctgt
     6721 ttttattgat acatcagata cccgtgatat ggacgtccgg atgaatgaag tggccgaacg
     6781 cctgaataat gaactgaagg aaaaatgttt tgcagcacta acgcccaata caaacaatac
     6841 tttggtgaac agcccggtat tcagcaagct gatgaatgaa agcggcgata tccggatctg
     6901 gaacagcgga gttaaaccgg caactaccaa agagatccaa caacttcctc catttgtgac
     6961 ccggtttttg tcaaccctgc aagctgcgca gggaactgaa agcaccagta ttgtcaattt
     7021 tgaaaatggt aaaatagcgg gggcgatacg caattatata agtccggaaa tgggctccat
     7081 ctaccaaaaa tatccgcagg agccactacc cactcaactg gtcagccggt tgccacaggg
     7141 aaaaatcctg atgctgatga tgagtgcttt caataaggat atgtcaggtg agatcagcaa
     7201 gcaaaacggg atggccgggt ttatagacag cctgaaacaa tacctgaaag ttgatcttag
     7261 tctgtttcag aaaggatttc aaaacaaagc gatgatggcc attatggaaa tgccggcgaa
     7321 ggaagatgat aacggggaga agaaaaaaat cctggaaaac ctgggattat ttattgtgat
     7381 ccctgtagct gataaagctg ctgttgccca actgaaagca ttggccgatc gtaaaatgga
     7441 ttcattggca gctacagaaa aaggagaaaa gatcatgagc gtcttccgtc ctgcaattat
     7501 gtataatgac agtctttgtg taattgccca ctctgctgcc atggccgaag cttatctcaa
     7561 ccattccggt aatgcccccc tgccggcctg gttaccgtcc aatcaaaaag gaaatatgtg
     7621 gatgagcctt ggcttcaggg atatcatgac gatggttttt agtatggctg gaaagccggg
     7681 gaaatctaaa aacaaccaga cgatggagat gctggagatg tttgatcagc tggtcattac
     7741 cggaggtgac tatgccgaag gaagtatgaa tagccagatg gagttccgct ttagcaaccc
     7801 cgataaaaat gtgctggaac aactatttga aatggccaat cgtgccagta tgcaaaaggc
     7861 aaaagcgaaa aaagaaatgg ctgacacgcc tgaaattatg caggatgatg ttgtaactga
     7921 tgcgccgaaa gaagccccgc cagcacctaa aaaagcaccc gttaaaaaac cagtcagcaa
     7981 atcaaaaacg cccgctaaga caaaagggta ataacaattt tctataaaca aaagcccgtc
     8041 agtattgata ctgacgggct tttttacttt tgacttttta attttcactt accactgtac
     8101 aacctctctc tttctgagaa aatatccaat cccctcttct gttttcgttc cttccatatt
     8161 tacatcttcc acctgtatct gcaaaccatt tactttcccg tccgcattcc gtttaaaagt
     8221 ggcggtaccg gcatacacca ccagttcaaa tacatcctta ttatctgttt tacgcaattc
     8281 ggagttgccc attacggaag tggcggtgag cacaccctcc tgcacaatca ccccgatttc
     8341 cttaaccggg ctgccatccg gaaaaatata tttacccgtg tattgttgaa ataaagtatc
     8401 cgtctgggcc tgagctgcag tataggccag aaaggcgaaa agagcgagca caattttttt
     8461 catatatgtg gtatttattt caaattaaac gaaaaaaagc gccaaaggtt acgccttgtg
     8521 ttaatttttc agcatttctc ataaatgatg gcagctccct gaccaacccc tacacacata
     8581 gtagccagcc cgaaacgagc attccttctc cacagttcgt gcagcagcgt ggcggatatc
     8641 cgtaccccac tgcagccgag cggatgacca attgcgatgg cccctccatt tacattcact
     8701 ttttcaggat ccaattccag ctcacgcata caggctaagg attgggaggc aaaggcttca
     8761 ttcagttcca ccaggtccat ttttcccatc gacaggccag cccgttgcag ggctttttgg
     8821 gtagccggaa ccggaccgat tcccatgatt gcaggatcta cacctgcagt agccatcgaa
     8881 cggatacggg ccatcggctg aagtccatat ttttttacag ccccttcact ggccagtaac
     8941 atagccgcag aaccatcatt gataccggat gaattacctg ccgtaaccgt tccctcttta
     9001 agaaaagcag gttttaatgc cgccagtttt tcaagactgg tttccctggg atgttcatca
     9061 ttcacgaccc aactgaccag tcccttttga gtcagttcca cgggggttat ttcatcggcc
     9121 catttatcag cggcctttgc agctgcatat ttctgttgcg aacccagggc aaatacatcc
     9181 tgttcttccc gactgatctt ccattgactg gccacattct ccgcggtttc acccatggta
     9241 aaggggtgat atatggaagc cagggtttta ttgataaagc gccagcccat cgtggtatca
     9301 taaatttcgg ctgttcggcc aaaggcttcg gtggctttag gttgtacaaa gggggcccgg
     9361 ctcatacttt ccaccccggc tgcaatcacc agttccgcat ccccgcaggc aatctgccgg
     9421 gctccgtcca taatggcctg gaggccggaa gcacagagac ggttgaccgt attgccaccc
     9481 accgttacgg gtaaaccagc cagcagggcg gccattcggg ccacattccg gttatcttcc
     9541 ccggcctggt tggcggcacc ggcaattacg tcctcaattg cggccggatc tacggtctca
     9601 ttccggcgaa gcaaggcccg gatgatataa gccagcaaat cgtcgggacg gatactgcta
     9661 agaatgcctc cgtaacggcc gacaggcgtt ctgacagtat caatgatata tacagggttc
     9721 atgctccttg atttgaagca aatatccggg aattcattta atcccggccg catggttatc
     9781 tttgccccgg atgaaaaaga cacagaaaaa catacggcat cttaccctcg cggaaattga
     9841 aacctttttt gctgaactgg gagaaaaaaa attcagggcc aaacaggtct atgaatggct
     9901 ttggcagaaa ggcgccctga gttttgccga tatgacgaac ctgagcaaag acatgcgcca
     9961 gttgctggga gaacaattta ccttccccag tctgaccatt gatgccaccc aaatttcaga
    10021 agatggcacg atcaaatcac gcttcaaaac atgggacggt cacctggtgg aaggagtcct
    10081 tatccctaca acggcccgtt ataccgcctg cgtatcctcc cagatcggtt gttcactgag
    10141 ctgtaaattc tgtgcaaccg ggtatatcga ccggaagcgt aacctggaat ttgatgagat
    10201 ctatgatgaa gtggtgttaa ttaatcagca gagtgaaagg gtacatggca aaaaactgag
    10261 caatatcgtt tttatgggca tgggtgaacc cctgctcaat tataaaaatg tgatccgatc
    10321 catcgaacgg atcacttctc ccgacggatt agccatgagt ccccgccgga tcaccgtttc
    10381 taccgccggg gtggccaaag gaatccgcca gctgggggat gataaagtga aattcaaact
    10441 ggctttatca ttgcatgcgg ccactgatgc caagcggcat gagatcatgc cgataaatga
    10501 cacgaacaat ctcaagacgc tgacggatgc cctcaattat ttctacaaac aaaccggcaa
    10561 tgagattacg tttgaatata tcctgttcaa ggatttcaat gattcgttga aagacgcgga
    10621 cgacctgatc aagatttacc gccaggtgcc ggccgacctg atcaatatca tcgaatacaa
    10681 tcccatcgaa ttcgccaaat acgaaaagcc tgaagaagcc aaggtgcagg ccttcatgca
    10741 atacctggag aagcaccggg tgaatgtccg gttacggaaa agcaggggga aggatattga
    10801 cgcggcatgc ggacagctag ctaataaagg gtaggttaac aggttgaaaa gttgataggt
    10861 tgacaaggga ccttctaagg aaaccgcctt gtcaacctat taacttgtta acctgtcaac
    10921 attaacaacc tcctgaatta ttgttaaaca ccctcagcag aaaataatta cgaattattt
    10981 cttagttcgg ttaaacccgg gcctttcctg ccggtctcta ccctattcac attaattaac
    11041 cttgaccggc gaaggttatc cattcaggcc ctcgtctcaa agaaaaagta aaacatgaaa
    11101 aaattaatgc tccccgtact ggctatcgca cttgtattca gtgccaatgc ccaggaaatc
    11161 cctgaaagaa aagcagacca tccacccata atgcatcata agcagaaagg aggaccggaa
    11221 cgccataaag ccatgaaaga actgaatctg actgatgcac agaaagaaca gttcaaaact
    11281 gaaaaagatg ctttccgtaa aaaaatggaa gagctgaaaa agaatgataa cattacggtg
    11341 aaggaatgga aggcgaaaaa agaaagcctg gtgaaagaac ataaggcgaa gatggaaagc
    11401 gtgttcacgc ctgaacaaaa ggccaaaatg gaacagcgta aaaaagaggg acaggccaaa
    11461 cagcaggata tgatgaaaca aagagcggag aaaatgaaaa cacagctcgg actgacagaa
    11521 gagcaatccg ctaaactgga aaagagccgc aaggaaattg gtgataaaat gaaagcgatc
    11581 cgtgagaaca agtcgctgac tgaagaacag aaacgcgagc agatgcgaga actcggtaaa
    11641 aagcaaaaag agaacatgaa gtctatcctt accgaggagc aactgaaaaa aatgaaggaa
    11701 ggaaaaaaac aaggacccgg tcgtggggaa ggtcccggta aaggtcctaa ggggccaaaa
    11761 gggcataaaa ctcctccgcc tcctcctgtt gaaaaagaaa ccctttgatg attctatgat
    11821 atgatgtaaa ggccccggat cggggccttt tttttgcctg cggcaaaaag gggtggccac
    11881 gaaatcacac gaaagcccac gaaggccccc taaatccccc gaagggggac ttcagacgcc
    11941 gatttataat aaagtcattt aatgttttga attgcgtatg tagtcccgca aagccaaaaa
    12001 gagttttcga ttttcggaat gtcttataaa gctttgcg
//
LOCUS       DAOPMW010000062        12014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5566_length_12014_cov_7.909524, whole genome shotgun sequence.
ACCESSION   DAOPMW010000062 DAOPMW010000000
VERSION     DAOPMW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5566_length_12014_cov_7.909524"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..929
                     /gene="accD"
                     /locus_tag="PLU11_05070"
     CDS             15..929
                     /gene="accD"
                     /locus_tag="PLU11_05070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPN58517.1"
                     /translation="MAKQIEDFDANLSGEEGKAEETRASWFKRIKKGINTKTSEKKET
                     PEGLWVKCPECNFICTITELKENVFVCPKCGYHHRINSQEYFEILFDNGEFTELFENI
                     RSKDFLEFTDLKPYKKRLEEIWSKTDLKDSMRVAVGKVGGNDLVVACMDFEFIGGSLG
                     SVMGEKFSRAVDHCIGNQMPYMVISKSGGARMMESAFSLMQLAKTSGKLSQLSDAKLP
                     YISLLTDPTFGGISASFGMLGDLNIAEPGALIGFAGPRVIKETIKKDLPEGFQRSEFI
                     LEHGFLDFIVPRKELKNKLASVLVLFKN"
     gene            1002..1439
                     /gene="smpB"
                     /locus_tag="PLU11_05075"
     CDS             1002..1439
                     /gene="smpB"
                     /locus_tag="PLU11_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPN58518.1"
                     /translation="MAELRNRQAYYDYFIDIKYEAGMVLLGTEVKSLRGGRASFNDSY
                     CLVHKGEIWVKSLHIAEYTHGTVNNHDPLRDRKLLLEKREIRKIEAKLKEKGYTLIPL
                     RIFFNSKSLVKMEIGLARGKKLYDKRESIKQKDVEKEMKRYLK"
     gene            1494..2417
                     /locus_tag="PLU11_05080"
     CDS             1494..2417
                     /locus_tag="PLU11_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58519.1"
                     /translation="MKKGFLIFLLLFTLQGSSQTVFGYWYGFANVKSNSSANNYLVEM
                     VLQPEKNYVNGIINYYFKNTYRSLQIKGSYNASTRQLIIYDVPVVYHGSSGNFEVDCI
                     MDMVGILRVSKAKTVINGIFSAKPEYKYTCSDISFSLTHDGDASKKDSVMKALSEFKE
                     NYQVWKPAEKDTIVAVKVIQRKVVNYVIERQFTERESELANEIEVDSDSLRIDLYDNG
                     EIDGDIVSLFYNKELILFNQKLTHKSIHIDIVLDSSKAENEITLFAENLGLIAPNTAL
                     MRVRDGKKIYDLRVSSSLEKNATIRIRKKRK"
     gene            complement(2570..5083)
                     /locus_tag="PLU11_05085"
     CDS             complement(2570..5083)
                     /locus_tag="PLU11_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPN58520.1"
                     /translation="MSAAKNKITDVQNNDSGLHGQYKSWFLEYASYVILERAVPAVED
                     GLKPVQRRILHAMKEMDDGRFNKVANIIGQAMQYHPHGDASIGDALVNMGQKDLLIDT
                     QGNWGDVRTGDDAAAARYIEARLSKFALDVAFNAKTTEWQLSYDGRKNEPVTLPMKFP
                     LLLAHGADGIAVGLSTKILPHNFGELIDAAVKYLRGRKFDLFPDFLTGGMIDVADYNQ
                     GKRGGKVKVRAHIEEVDKKTLAIKSVPYGVTTTQMMESIVKANDQGKIKIKKVTDNTA
                     AEVEIQVDLAPGISPDITIDALYKFTDCEVSISPNACVIVEQRPQFLGVQELLKLSVD
                     RTKDLLEQELKIRLAELQEKWHYTSLEKIFFEEKIYKELEKKHETWDKVLESIDKAFT
                     PFKKLLKRDISREDIIKLTEKPVRRIYKLDIDELNAQIKGLEADIRQVKNDLANLVDY
                     AVAYYENLLKKYGKGRERKTEIKAFEVIEAKHVAIANTKLFVNRAEGFIGTGLKKDEL
                     LFECSDLDDIIVFAKRGIMKVVKVSDKAFIGKEILHAAVFQKNDERTTYNMIYLDGAT
                     GMSYAKRFNVTGVTRDKEYDLTKGHEKSKIHYFTANLNGEAEVVKIVLGNCTARIKDF
                     DYYFEELEIKGRNSQGNQVTKYPIKSVKFKEKGRSTIAGKKLWYDDQFGRLNSDEKGS
                     YLGVFESEDKILVMFRDGNYEITDQELTQKLDSEKVIFIEKFKPERIVTAVYVDMEKK
                     QFMVKRFKIETTTLKNKFSFIKEGEGNYVEAVTTIDDPILAMQQGRGAQIRKGKLKMN
                     KIAEITGWRTVGTKLADYSKSTEMEWVKPKEEDNQPELF"
     gene            complement(5088..5552)
                     /locus_tag="PLU11_05090"
     CDS             complement(5088..5552)
                     /locus_tag="PLU11_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPN58521.1"
                     /translation="MANTYAQIYLQIEFAVQGREARIHESFRDELQMYMSGIISKNGQ
                     KLYAIYCMPDHTHILISYKPGLMLIADLVRDIKSCSTAFLKSKGFVRTGFSWQSGYGV
                     FSYHKELLPIVTAYILNQPAHHEKKKFQQEYLEFLNEFEIEYNERYLFEFYE"
     gene            complement(5964..6815)
                     /locus_tag="PLU11_05095"
     CDS             complement(5964..6815)
                     /locus_tag="PLU11_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HPN58522.1"
                     /translation="MTHIVFNESEVALMQEVMTLDETMSGEVLLIRDDFAVGPLTQLD
                     TEEGWLARLSWWNQISQGSPYGEKTGSFDDRQTVAAIKEKLTANPEEELWIWMGQNQH
                     DVTGYYWLMPQLKEFQGRIMILYMNNLPFINEKGHIFYPSWLSEIQPREFTKAKKLCR
                     PITLSEFEIDPDEWRKLTEENAVVRMLEGGKKIAGKEDSFYDAEIIKNITGEWQKATR
                     VLSNTLNRMKIKTGDIFLMWRMKQLITDGKIEITGDPSKSWKDFDVKLAGARQVVEAG
                     EDSVPVS"
     gene            complement(6832..7575)
                     /locus_tag="PLU11_05100"
     CDS             complement(6832..7575)
                     /locus_tag="PLU11_05100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58523.1"
                     /translation="MNAEKYVIPFIEEKFPLQPGMRVLEIGCGEGGVLKAFINRGCEG
                     VGVELDAPRIDHAREYLPDDIAAGRLRFVVKDIYLVDIEKDFSGLFDIILLKDVIEHI
                     HDQPKLIGWMKKFLKPGGIIFFGFPPWYMPFGGHQQICKSKISRLPYIHLLPGSLYKA
                     ILKNRKENVEEMMEIRETGISIERFERICREQGYGFLDKRHYLLNPIYEWKFGWKPKK
                     QLGLIKAIPFFRNFLTTCVYYCIQSTPDS"
     gene            complement(7626..9524)
                     /locus_tag="PLU11_05105"
     CDS             complement(7626..9524)
                     /locus_tag="PLU11_05105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPN58524.1"
                     /translation="MAKDKDTTNLFDYTEDSIKSLDWKEHIRLRPGMYIGKLGDGASP
                     DDGIYVLVKEVMDNCIDEYTMGYGKAVELVIEGKTVTVRDYGRGIPLGKVVDVVSKIN
                     TGAKYDSKAFQKSVGLNGVGTKAVNALSHYFKVTAIRDGKEKTAEFERGVLIKEHKEA
                     KTAEPNGTVVTFIPDETVFKNYKFLPEFLENQIWNYCFLNAGLKIHFNGKTFLSKNGL
                     LDLLQRKTNEDEIRYPIIHITGEDIEVAITHNNDYGEDIYSFVNGQHTTQGGTHQQAF
                     REAYVKTIRDYFKKDYDASDIRTGIVAAVSVRVVEPVFESQTKTKLGSVNVDIEGPSM
                     KQFVGDFLSRELDNFLHKNPTIAESLKKRIEQSERERKELAGIKKLANERAKKANLHN
                     RKLRDCRYHLDDDAPAKNKEEYVAKQKETTIFITEGDSASGSITKARNVETQAVFSLR
                     GKPLNCFGLTKKVVYENEEFNLLQHALNIEEGLEGLRFNNVVIATDADVDGMHIRLLL
                     MTFFLQFFPDLVKHEKVYVLETPLFRVRNKQETIYCYDESEKQAAMKKLGTKPEVTRF
                     KGLGEISPDEFAQFIAQDMRKQLMRVEEGDHIQHLLEYYMGKNTPDRQEFIISNLRIE
                     TDKVEEPA"
     gene            9762..10376
                     /locus_tag="PLU11_05110"
     CDS             9762..10376
                     /locus_tag="PLU11_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPN58525.1"
                     /translation="MAGTTYKSIHPKNRKAWRKWLENEHLSSTGIWLVYYKKHTGLRG
                     LSYDEAVEEALCFGWIDSLPGKLDDDRSMLKFTPRKPRSVWSALNKKRVDQLISQGLM
                     TAAGLASIELAKKNGSWESLTASDEAAALNQVPPDLAKLFARNKQAGEHFMAFSQSVR
                     KQFLSWIFSAKRPETRKQRLAQTVKMSAANVKPGAQGFKIVSSR"
     gene            10706..10975
                     /locus_tag="PLU11_05115"
     CDS             10706..10975
                     /locus_tag="PLU11_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58526.1"
                     /translation="MYTYDLLETGCYYLVKEKEDSPVILIRVAVTTDHCLFVQRYDEP
                     METCWRMKKDPLFDIIECLSDAAVKSWEVYYRSSQDAFFGEDDEE"
     gene            11042..>12014
                     /locus_tag="PLU11_05120"
     CDS             11042..>12014
                     /locus_tag="PLU11_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPN58527.1"
                     /translation="MQRTTFSRSFFLSLFLLLSGLINAQEDFLPNDYLGKEFYAGRRD
                     ALRAAMPDSSVAVIFAYPTRTFSNDVEYLYHANPDMYYFSGYKEPHSMLILFKETQTD
                     STGKSYKELLFVQKKNPQAEQWTGKRLGTDGAREKLGIQDVYNGLDFKNFPIDFSRFK
                     KILHDRLPADLVNTNDRADLYDLVDQFRQKLNLTDKAMQNKSDTRQYFELTGKLRRIK
                     TPEELSLLRKAVEISCQAHNEVMKTIHPGMSELEIQGLQEYVHKKQGAEGVGYGSIVG
                     SGENGCVLHYMENTKTKIGGSLLLMDVGAEYHGYTDDVTRTVPADGKY"
BASE COUNT         3196 a         2788 c         2564 g         3466 t
ORIGIN      
        1 ttcaagattc ggttatggct aaacaaattg aagattttga cgcgaacctg tctggcgagg
       61 aaggcaaggc tgaagagaca cgggccagtt ggtttaagcg tatcaagaaa ggaattaaca
      121 ccaaaacttc ggagaaaaaa gaaacccccg aagggctttg ggtcaaatgc ccggaatgta
      181 attttatctg cacgattact gaattaaagg aaaatgtctt tgtttgtccg aaatgtggtt
      241 atcatcaccg gatcaacagc caggaatatt ttgaaatcct gtttgataat ggcgagttta
      301 cagagctgtt tgagaatatc cgaagcaagg attttcttga atttaccgat ctcaaaccgt
      361 ataagaaacg actggaagaa atctggagca aaacggatct gaaagattcc atgcgggtgg
      421 cggttggtaa agttggcggg aatgatttag tggtggcctg tatggatttt gaatttatcg
      481 gcggctcact gggcagtgtg atgggggaaa aattttcaag agcagttgat cattgcatcg
      541 gtaatcagat gccctatatg gtaatcagta agagtggtgg tgcccggatg atggaaagtg
      601 ctttttcgct gatgcaactg gcaaaaacca gtggtaaact ttctcagctc agcgacgcaa
      661 agcttcctta tatttcactg ctcactgacc cgactttcgg aggtatttca gcctctttcg
      721 ggatgctggg tgacctgaat attgccgagc ccggagcgct gatcggtttt gcaggtccaa
      781 gggtaatcaa ggaaacaatc aaaaaggacc ttccggaagg tttccagcgg agtgaattca
      841 tcctggaaca tggtttcctg gactttattg taccccgtaa ggagttaaaa aacaaactgg
      901 cttccgtact ggtgttattt aaaaattagt cttaacagaa cgaataagcg ataagaggta
      961 actttgccgt ccggcagaag cctcttattt tatttttatc catggcagaa ctcagaaaca
     1021 ggcaggcata ttatgactac ttcatcgaca tcaagtatga agcggggatg gtattactgg
     1081 gtacagaagt aaaatcatta cggggcggca gggccagttt taatgacagc tattgcctcg
     1141 tgcacaaggg agaaatctgg gtcaaatccc tgcatattgc ggaatatact catggcaccg
     1201 tcaataatca tgatcccctg cgcgaccgca aacttttact tgaaaaaaga gaaatccgga
     1261 aaattgaagc caagctcaag gaaaagggat ataccctgat ccctctccgg atattcttca
     1321 acagcaaaag cctggtaaaa atggaaatcg ggctggcccg tggtaaaaaa ctgtatgata
     1381 aacgggagag catcaaacag aaagatgtag agaaggaaat gaaaagatac ctgaaataac
     1441 cctgtgtaaa acttcctttt tttaaggcat gccttttgcc gatattacaa actatgaaga
     1501 aaggatttct gattttttta ctccttttta ccctccaggg ctcgtctcag accgtctttg
     1561 gttactggta tggatttgcc aatgtaaaga gtaacagctc cgccaataat tacctggttg
     1621 aaatggtgtt gcaaccggaa aagaattatg taaatgggat tataaattat tatttcaaaa
     1681 atacttaccg tagtctgcag atcaaaggca gctataatgc cagtacccgc cagctgatca
     1741 tttatgatgt accggtagtg tatcatggtt catccggaaa ttttgaagtg gattgtatca
     1801 tggatatggt cggaatatta cgtgtatcca aggccaaaac ggtaatcaac ggtatatttt
     1861 ctgcaaaacc agaatataaa tacacctgtt cggatatttc attcagcctt acccatgacg
     1921 gggacgcgag taagaaagat tctgtcatga aagccctgag tgaattcaaa gaaaattacc
     1981 aggtctggaa accggccgaa aaagacacca tagtagcggt caaagttatt cagcggaaag
     2041 tggtaaatta tgtaattgaa agacagttta ccgaaagaga gtcagaactg gccaatgaaa
     2101 ttgaagtgga ttccgattcc ctccggatag atctctatga taatggggaa atagacgggg
     2161 acattgtatc cttattttat aataaagaac tgatcctctt taaccagaaa ctgacccata
     2221 aatccattca tatagatatt gtactggatt cttcaaaagc cgaaaacgag atcaccttat
     2281 ttgcggaaaa tcttggattg attgcaccca ataccgcgct gatgcgtgtc cgggacggaa
     2341 aaaaaatata tgacctgagg gtgtccagca gcctggagaa aaatgctaca atacggatac
     2401 ggaagaaacg aaaatgagta atgaataatc agtaatcagt aatgagtaaa tatcgaagag
     2461 tgaatataaa attaaaccct ccgtttccgg agggtttaat tttattaacc attaccagta
     2521 acacgattta ctcattactc attactgatt actcatttct cgtttctcat taaaataatt
     2581 ccggctgatt gtcttcctct ttcggtttca cccactccat ttctgtggat ttgctgtaat
     2641 cggccagttt ggtccctact gttctccagc cggtaatttc ggcgatttta ttcatcttca
     2701 gttttccttt ccggatctgt gcgccccttc cctgctgcat cgccaggatg ggatcatcta
     2761 tagtcgtaac ggcttccaca taattgcctt ctccttcctt gataaaagag aacttattct
     2821 tcagtgtggt ggtttcaatt ttaaaccgtt tcaccataaa ctgtttcttc tccatatcca
     2881 catatactgc cgtcacaatc cgctccggtt tgaatttctc gataaagatc actttctctg
     2941 aatccagttt ctgggtcagt tcctggtcag tgatttcata attaccatcc cggaacatca
     3001 ccaggatctt gtcctccgat tcaaacacac cgaggtagga tcctttctca tcgctgttca
     3061 accgtccaaa ctgatcatca taccagagtt ttttaccggc aatggtgctc cggccttttt
     3121 ccttgaactt aacggattta atgggatact tggtgacctg gtttccctgg ctattgcgtc
     3181 cctttatctc cagctcttca aaataataat caaaatcctt gatccgcgcg gtacaattgc
     3241 ccagcacaat cttcaccact tcggcctccc cgttcagatt cgcggtgaaa taatgaattt
     3301 ttgatttttc atggcccttg gtaaggtcat actctttgtc ccgggttacc ccggtcacgt
     3361 taaatcgttt ggcataactc atcccggtcg ctccatccag gtagatcata ttataggtgg
     3421 tgcgctcatc attcttctga aagaccgccg catggagaat ttccttcccg ataaaggcct
     3481 tatcggacac tttaaccact ttcataatcc cccgtttggc aaacacaatg atatcatcca
     3541 ggtcagaaca ctcaaacagg agctcatctt ttttcaaacc ggttccgata aatccttctg
     3601 cccgattgac gaacagtttg gtattggcaa tggccacgtg tttggcttcg attacttcaa
     3661 aagctttgat ctcggttttt cgctcccgcc ccttaccata tttcttcagc aggttttcat
     3721 aataggccac cgcataatcc accaggttag ccaggtcatt tttcacctgc ctgatatcgg
     3781 cttccagtcc tttgatctgg gcgttcagtt catcaatatc gagcttataa atccggcgta
     3841 ccggtttctc tgtcagcttg ataatgtctt ccctggaaat atcccttttc aatagtttct
     3901 taaagggggt aaaagcttta tcaatcgact ccagtacttt atcccaggtc tcatgttttt
     3961 tctccagttc cttgtagatc ttttcctcaa agaagatttt ctccagcgaa gtgtaatgcc
     4021 atttttcctg taactcggct agccggatct tcaattcctg ttccagcagg tccttggttc
     4081 tgtcaactga tagtttcaat aactcctgta cgcccaggaa ctgcgggcgc tgctccacga
     4141 tcacacaggc attcggggaa atggatactt cacagtccgt aaatttatac aaggcatcaa
     4201 tggtaatatc cggagaaata ccgggagcca ggtccacctg gatctccact tccgctgctg
     4261 tattatccgt tacttttttg atctttatct ttccctggtc attggccttt acaatggatt
     4321 ccatcatctg cgtggtggtt accccgtaag gcacactttt aatcgccagt gttttcttat
     4381 ctacttcctc gatatgggcc cttactttta ccttccctcc tctttttccc tggttatagt
     4441 ctgccacatc aatcatcccg cccgtcagga aatcaggaaa caggtcaaac ttgcggccgc
     4501 gcaggtattt gaccgcggcg tcaatcagtt caccaaaatt atgcgggaga atcttggtcg
     4561 ataaacccac ggcaattcca tcggctccat gagccaggag cagggggaat ttcatcggca
     4621 gggtcaccgg ttcgtttttc cgtccgtcat aactgagctg ccattccgtg gttttggcat
     4681 tgaaggccac gtccagggca aatttgctca ggcgggcttc aatgtaacgg gcggctgctg
     4741 catcatctcc ggtacggaca tctccccagt ttccctgggt atcaatcagc agatcctttt
     4801 ggcccatatt taccagggca tccccgatac tggcatcccc gtgtgggtga tactgcatgg
     4861 cttgtccgat aatattggct actttattaa aacgaccatc atccatttcc ttcatcgcat
     4921 gaagaatccg gcgttgtacc ggtttcaacc catcttccac ggcgggcacg gccctttcca
     4981 ggattacata ggaggcatac tcgaggaacc aggatttgta ttgcccatgt aagccgctgt
     5041 cgttgttctg tacgtctgtt attttattct ttgctgcgct cataatgcta ctcgtaaaat
     5101 tcaaataaat atcgctcgtt atactctatt tcaaattcgt ttaaaaattc caggtattct
     5161 tgttggaatt tctttttttc atgatgggcc ggttggttca ggatataggc ggtgacaatg
     5221 gggagtaatt ctttgtgata ggaaaaaacg ccatagcctg attgccagct gaatccagtc
     5281 ctgacaaacc cttttgattt caaaaaagcc gttgaacagg atttgatgtc ccgtacaaga
     5341 tcagcaatca acatcaatcc gggtttatag ctaattaaaa tatgtgtatg gtcaggcatg
     5401 caataaatgg cataaagctt ctggccattt ttgctgataa tacccgacat atacatttgt
     5461 aattcatccc ggaaagattc atgaattctt gcctcccttc cttgcaccgc aaattcaatc
     5521 tggagataaa tctgtgcata cgtattagcc atgaattata atttaactgt gaatattaca
     5581 taatatatcg cccctacagg gctccctggc catttaccaa tgctggctaa tcatatgcca
     5641 cccctacggg gcaaacagcc atttttcgcc gggtatatgt atataaacgt agtgttcaca
     5701 attaaaaaac acctgatcaa agaactgttt taaacagtgc ttcataaaat acccattgaa
     5761 aagaaccaga caaatgtcat aggtttaatt caacgtcgta tgttatttca aacaccgttt
     5821 catgttattt ttcccgcgaa attctgttct cccaaaactc taaaaacagt taaagcaacc
     5881 agaattactg ctaaacccac agagccccag cggggcgaaa tatcatatgc acagcccatc
     5941 caaaccccag cggggcggcc tgtttaagac accggcacac tatcctcccc ggcttccacc
     6001 acctgcctcg cacctgccag cttcacatca aaatccttcc atgattttga cggatcgccc
     6061 gtgatctcga ttttaccatc cgtaatcagt tgcttcatcc gccacatcag gaaaatatct
     6121 ccggttttaa ttttcatccg gttgagggta ttgcttaata cccgcgtggc tttctgccat
     6181 tctccggtta tatttttaat gatttccgcg tcataaaaac tgtcttcttt cccggctatt
     6241 tttttgcccc cttcgagcat ccgcaccacc gcattctctt cggttaattt tctccattca
     6301 tccgggtcaa tttcaaattc actgagtgtg atgggacggc ataacttttt tgctttggta
     6361 aattcacggg gctggatttc actcagccag gaaggataga atatatgtcc tttctcattg
     6421 atgaagggca ggttattcat ataaaggatc ataatccgtc cctggaattc tttcaattgc
     6481 ggcatcagcc agtaataacc ggtcacatca tgctggtttt gccccatcca tatccacaat
     6541 tcttcttccg gattcgccgt cagtttttct ttgattgcag caaccgtttg ccggtcgtca
     6601 aaacttccgg ttttctcccc atagggagaa ccttgggata tctggttcca ccaggacagc
     6661 cgggcaagcc agccctcttc agtatcgagt tgagttaaag gacctacggc aaaatcatcc
     6721 cggatcagca ggacttcacc ggacatggtt tcatccaaag tcattacttc ctgcatcagg
     6781 gctacttcac tttcattaaa aacgatatgg gtcatattgg gggattgggg attaggaatc
     6841 aggggttgat tgaatacagt aatacacaca ggtggtcagg aaattccgga aaaacggaat
     6901 agcctttatc aggccaagct gtttcttagg tttccacccg aacttccatt catagatcgg
     6961 attaagcagg taatgacgct tatcgagaaa tccatatcct tgttcccggc agatgcgttc
     7021 aaaccgttca atgctgatgc cggtctcccg gatttccatc atctcctcca cattttcttt
     7081 ccggtttttc aggatggctt tgtagagaga gcccggcaac aaatggatat aaggtaagcg
     7141 ggagattttt gatttacaaa tctgctgatg gccgccgaag ggcatatacc atggtggaaa
     7201 accaaagaag atgatgccgc ccggtttcag aaactttttc atccagccga tcagtttcgg
     7261 ttggtcatga atatgttcga tcacatcttt gagcaggatg atatcaaaga gcccgctaaa
     7321 atccttttct atatctacca ggtagatatc ttttaccaca aaacggagac ggccggcggc
     7381 tatatcatcc ggtaaatatt ccctggcatg atcaatgcgc ggggcatcca gttccacgcc
     7441 cactccttca catccgcggt tgatgaaagc ctttagcacc ccgccttcgc cgcaaccgat
     7501 ttccaatacc cgcataccgg gttgcagggg aaatttttct tcaataaaag gaatgacata
     7561 cttttccgca ttcataacct gaatgtcaaa atatcttttc ctatcagcat gaaattcaaa
     7621 cataatcagg caggttcttc cactttatcc gtttcgatgc ggagattact gataataaat
     7681 tcctggcggt ccggggtgtt tttccccata taatattcca gcaggtgctg aatatgatcg
     7741 ccttcttcca cgcgcatgag ctgtttgcgc atatcctggg cgataaactg ggcaaactca
     7801 tccgggctga tttccccaag tcctttaaag cgggtgactt ccggtttggt ccccagtttt
     7861 ttcatagctg cctgcttttc actttcgtcg taacaataga tggtctcctg tttattcctg
     7921 accctgaata gcggggtctc caacacatac actttttcgt gttttaccag gtcgggaaaa
     7981 aactgaagga aaaaggtcat taacaacaga cggatatgca ttccatccac atcggcatcg
     8041 gtggcgatca ccacattatt gaaacggagt ccttccagtc cttcttcgat attaagcgca
     8101 tgttgcaaca ggttgaactc ttcattttcg tatactactt ttttggtgag gccaaaacag
     8161 ttgagtggtt taccccgcag actgaatact gcctgggttt ccacattccg ggccttggtg
     8221 atggatccgc tggcactatc cccttccgtg atgaaaatgg tggtttcttt ttgcttggct
     8281 acatactctt ctttgttctt ggcgggtgca tcatcatcca ggtggtatcg gcaatcccgg
     8341 agtttacggt tatgcaggtt agcctttttc gcccgttcat tggccagttt tttaatacca
     8401 gccagctctt tgcgttcccg ctcgctttgt tcgattcttt tcttcagcga ctcggcaatg
     8461 gttgggtttt tatgaaggaa attatccagt tcgcgggaca aaaaatcacc tacaaactgt
     8521 ttcatactcg ggccttcaat atccacattc acggaaccca gtttggtctt tgtttgtgat
     8581 tcgaataccg gttccaccac ccgtacagag acggccgcca cgatacctgt acggatatcg
     8641 gaggcatcgt aatccttctt aaaataatca cggatggttt taacatacgc ttcccggaaa
     8701 gcttgctggt gcgtaccccc ctgggtggta tgctgaccgt ttacaaagct gtaaatatct
     8761 tcgccatagt cattgttgtg cgttatggcc acttcaatat cctcgccggt gatatggata
     8821 atcgggtagc ggatttcatc ttcgttggtc ttccgttgca gcagatccag taacccgttt
     8881 ttgctgagga aggtcttgcc attgaaatga attttcagcc cggcattcag gaaacaatag
     8941 ttccatatct ggttttcgag aaattcggga aggaatttat aatttttaaa aacggtttcg
     9001 tccggaataa aagtgacgac ggttccattc ggttcagcgg tctttgcttc tttatgttct
     9061 ttgatcagca cccctctttc aaactctgca gtcttttcct ttccatccct gatagcagtg
     9121 actttaaaat aatgactgag ggcgttcacc gccttggtac ctaccccatt caatccgacc
     9181 gatttctgga aagctttact gtcgtattta gctcccgtat tgatcttgct taccacatcc
     9241 accacttttc cgagcgggat accccggcca tagtcacgga cggtaacggt tttcccttca
     9301 atgacaagct caacggcctt cccatatccc atggtgtatt catcaataca gttatccatg
     9361 acttccttca caagtacata gattccgtca tccggtgaag ctccgtcgcc cagtttacca
     9421 atgtacatac cgggacgtag ccggatatgt tccttccagt caagtgattt gatactgtct
     9481 tccgtatagt caaacaggtt cgttgtgtct ttgtcctttg ccatgatcac ggtacgttct
     9541 gaatagtaat gaaatgcctt tcgatttgct cactgccgac cggccattcc ggcccggtca
     9601 ttcgggcaaa tataccaaaa ccggcctgcc ggatgagatt tagagttatt cacagatggg
     9661 gataagaaat acctggattt ccgggataaa tcaaatgtgc aaatcggaca aaacagccat
     9721 ttcttttcat tgtaaaaaca gggttcttat taaatttacg gatggctgga actacctata
     9781 aatccataca cccaaagaac cggaaagcat ggagaaaatg gctggaaaat gaacacctgt
     9841 catcaaccgg catctggctt gtgtattaca agaaacacac cggtcttcgc ggcctttcct
     9901 atgatgaagc cgtggaagaa gccttgtgtt ttgggtggat tgacagtttg ccgggtaaac
     9961 tcgacgatga ccgctccatg ttgaagttta ccccccggaa acccagaagt gtctggagtg
    10021 cgctgaataa aaagcgggtg gatcaattga tcagtcaggg actgatgaca gcagccggac
    10081 tggcctccat agaactggcc aaaaagaatg gcagctggga atcgctgacg gcatcagatg
    10141 aggctgccgc acttaaccag gtgccacccg atctggctaa attatttgcc cgaaataagc
    10201 aagcgggtga gcatttcatg gcgttcagtc agagtgtccg taagcagttt ctgtcctgga
    10261 tcttcagtgc caaacgaccc gaaacacgta aacaaaggtt agcacaaaca gtaaagatgt
    10321 ccgcggcaaa tgtaaaaccg ggtgcccagg gatttaagat agttagtagt aggtagatag
    10381 tagttagtag tcggttgcta tatggagcag cgttttatga taggggaaaa tcttagtttt
    10441 taaagaacgg ctcttatcga ttaccttttt taatataaat attagcaaaa ttttatacta
    10501 atttgattat caatttatta actcattaat aaatattatc tttttctttt tcaaataaat
    10561 ttaaccaccc aaaactccgg gtttttgtaa ttttgctttt cacctgaccc tttagattct
    10621 tcccttaata ttaaggattt gaacatgtga agtcgggctt ttgtaaagcc aaggtgctgt
    10681 tgtccctgat ttaaaatgag gaattatgta tacgtatgat ttgctggaaa ccgggtgcta
    10741 ttacctggtg aaggagaaag aagattcccc cgtaatcctg attcgtgtgg ccgtaaccac
    10801 ggaccattgc ctgtttgtac agcgctatga tgagccgatg gaaacctgtt ggagaatgaa
    10861 aaaagatccc ctttttgata ttatagaatg cctgagtgat gccgctgtaa agtcctggga
    10921 agtttattac cgaagcagtc aggacgcctt tttcggggaa gatgatgagg aataatccgt
    10981 tcgtgtaaat tcgcccggtg cctgattgaa aaaagctatt ttagctgatc aatcaacgac
    11041 tatgcaacgc accacttttt cccgttcatt ttttttaagc ttgtttctgc ttctgtcggg
    11101 tcttattaac gcacaggagg attttctgcc caatgattac ctgggcaagg aattctatgc
    11161 cgggcggcgg gatgctttac gggcggccat gcccgacagt tcagtagcgg tcatattcgc
    11221 ctaccccacc cgtacttttt ccaatgatgt ggaatacctc tatcatgcca acccggatat
    11281 gtattatttc agcgggtata aagaacccca ttccatgctg atcctattca aggaaaccca
    11341 gaccgattcc actggtaagt cctataaaga attacttttt gtacaaaaga aaaatccaca
    11401 ggccgaacaa tggaccggaa aacggctggg gacggatggt gcccgggaaa aactgggaat
    11461 tcaggatgta tataatggcc ttgatttcaa aaacttcccg atcgatttca gccgttttaa
    11521 gaaaatactg catgaccggc ttccggcaga tttggttaac accaatgatc gggcagatct
    11581 ctatgacctg gtagaccaat tcagacagaa gctgaatctg acagataaag ccatgcaaaa
    11641 taaatcggat acccgtcaat actttgaact gaccggaaaa ctccgccgga tcaagactcc
    11701 cgaagaactc agcctcttac gtaaagcagt agagatttcc tgccaggcgc ataatgaagt
    11761 gatgaagacc atacaccccg gcatgtctga actggaaatt cagggcctcc aggaatatgt
    11821 tcataaaaag cagggggctg aaggcgtggg atatggttcc atagtcggct cgggcgaaaa
    11881 cggttgtgtc ctgcattaca tggaaaacac taaaacaaaa atcggggggt cactcctgct
    11941 gatggatgtg ggtgcagaat accatggata caccgacgat gtaacccgca cggtgccggc
    12001 tgatggtaaa tatt
//
LOCUS       DAOPMW010000063        12000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5579_length_12000_cov_6.611637, whole genome shotgun sequence.
ACCESSION   DAOPMW010000063 DAOPMW010000000
VERSION     DAOPMW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5579_length_12000_cov_6.611637"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="PLU11_05125"
     CDS             complement(<1..1729)
                     /locus_tag="PLU11_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPN58528.1"
                     /translation="MRSILLFFGILFTGTCLFPAMMNAQVKEPAATPINPQATSDSLR
                     DIQILGSDRFTILKVDDSTTLQILAGHVRLKQGSSLFNCDSCVLNNGIRIFEAWGNVH
                     IKDVDTVNITSSHLRYLIDKKIAYLDGGVKLTDGKGTLTTPDLEYDMNTEIGIYKNGG
                     RVVNKKSVLTSQEGWYYAGLRDIYFKKNVVMKDPAYDIYTDSLLYNTQSQSTRFIAQT
                     VIKDTSGRIIETSDGFYNMATGKAEFGKRPVIRDTKSRVYITGDQFAFNDSLQTSQAL
                     GNVVIIDSVQGTTILAGVVWQDKKNDRMLATNKPLMIIKQDNDSIYVTADTLFSARLS
                     DLYARKDSMVKDTMKGVQVINLGKNKGANKGDSLTAPQPIAPATDSLSKKEDELVLIA
                     SRQDSLTKDSVVAPVIVAADQLPPVADSSKKGMPAVQLTPAASSDSLAKRTATDSLSL
                     AKKDPNKKDSTDRYFEAYRNVRIFSDSMQAVSDSMFYSFKDSVFRLFQNPVLWSKGSQ
                     ITGDTVLLFTKNKKADRVKVFENSFLVNESEPGIYNQVRSIRMDGWFKEGSLDSVRAA
                     GYAECIYFIQ"
     gene            complement(1729..2718)
                     /locus_tag="PLU11_05130"
     CDS             complement(1729..2718)
                     /locus_tag="PLU11_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPN58529.1"
                     /translation="MKISNETKVGILTIVALVILIVGFNFLKGKDVFSRSKKIYAVFK
                     ELGTLEKSNEAKINGLPIGNVYDKKERDKDVSAVVVTISLTRDINIPKNSVAYISSSL
                     VGSSYIVIERGNSTEMMGDGDTLSTRMETGILGDVKAQMNPTMARIRDVLDSLKETLS
                     GVNGMLNMENKAYLKETLANLSQASNALNGLLDNKNGALAKTLNNAQAMTESLKNNSE
                     DITATIGNAKKVSEKLAALELQPTIDSLNTMISSLKATAAKLNSSDGTLGALMNDRAL
                     YDKLNNAILSAEILIDDLRTHPKRYVNLSIFGKKDKTGPLTSPAKKDTAAAAN"
     gene            complement(2791..3768)
                     /locus_tag="PLU11_05135"
     CDS             complement(2791..3768)
                     /locus_tag="PLU11_05135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN58530.1"
                     /translation="MKKVCIAFTLLTCFVWTTDLCAQDRKKPIQTIIIDPGHGGIDPG
                     APGMKSTEAQLALQVSLKLGDTIAKAWPDVKLVFTRTTDVLPGNKPTKDAALKYRADL
                     ANESGGDLFIAIHLNAAGHKPGGWYERRVVNKIPRTKTVKKGKKTYKKTYYEYEYENV
                     WVPNKAKGTETYVWAMNKNDDKVRSVNKTTDYTGEIDSTTTLSLPDPSDPSEKARMLI
                     YAQNYFRKSLNLADLVQQEFIAAGRVNRGVKQRNDKGIWVLQATGMPSILIEIGFISN
                     QEEEEYMNSEIGQAEIVANIFTAIKNYKEKLEIRAKPAAEVTEEATKGF"
     gene            complement(3807..4838)
                     /locus_tag="PLU11_05140"
     CDS             complement(3807..4838)
                     /locus_tag="PLU11_05140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN58531.1"
                     /translation="MIKRFFLAFLFLFISISFYSFRSGSPASAVKKQKVIKTIIVDAG
                     HGIMANGGHNGAKGSYSYEDEICLAVSKELVKKFHAEMPEIRIVESRPTDKITPIHRR
                     AEIANENKGDLFICIHVNAALPIKRSELIGHKTVTYYTGKGKNRKKQTKKVPQYRTHS
                     QPNPAKGTETYIWGSHKNEDKEIAMRENAPMLMEDNYKANYGEVDPNSPEFIALSLLK
                     TKQFFRRSATLAGLVQDEFSRVGRVDRDVRQRGVGIWVLQATAMPSILVETGYITNRA
                     EEDYLNSKEGQKELAGCIIRAVSGYIAWLEKQQQTNDQDNSGNSRYDTRNVSAMLNRI
                     EEMECSKVR"
     gene            5053..7749
                     /locus_tag="PLU11_05145"
     CDS             5053..7749
                     /locus_tag="PLU11_05145"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPN58532.1"
                     /translation="MPHHYFGTPLTINPDTTKPPVLKETPQPVTTNTDTTKRPVTDTN
                     QVNGTDTIPVRGTDTGYVQKTDTFNLKLSKDSLEAPLNYSAEDSVVVMIKSKKIYLYG
                     KTKTEYTDITLTAPKVEMDQATQVVTAYNRKDSTGKVLETAKFKQGENEFSSDTIRYN
                     FKTQVGLTKNTMIPQGEILILGESIKKVNENTSFIKRARFTTCMLDDPHFAFVTPKMK
                     VINQKLAISGPAHPEFEGVPVPIYVPFGFYPLSQGRHSGLLRPNFITDEVRGIGMQGL
                     GYYKVLNDYWDAEVSGDLFSYGEWAAGLKMTYRKIYKYSGGFNLTLRSSKQNFKGDPD
                     FSKTRIFNVTWNHSMDSRARPGVSFAANVNAGSTKFNQLVPGNAYLNYQNLMGSSITF
                     NKSWKNKPFNLSISATHSQNNNLGTVNLNLPNAAFTVSTLYPFQNKNRAGTAKWYENL
                     GIAYNGSFMNSVSFNDTIKYGQNGVKPFFQFLLDTAQWSAQHNIPITLSLPPILGGKV
                     LVSPGISYSQTWIQRTTNYQWNSALKKVDTITAKGIFIDQRSSISLSFNTALFGTVQF
                     KKSRVIALRHTVRPQIGMSYTPDLNTSHIKKTQVDTTGKVLYYNEIGGTVLYNTSSRR
                     SGAMSFGVDNNIEMKVRSKKDTGDLAIKRVKLIEGFGFSGSYDFIRDSFRLSPISFYF
                     RTTLFEKLNINASTTLNPYQTDSRGYDIAKYAWQGGKFSPGRLTSGSVSMSTNFQSKP
                     KDAKKDEARKKAVEERLQDPALLGDQQRLLEFMQQNPSQFVDFNIDWNLGLSYSLSFV
                     NRIKSDFSGYETDFQLGGVSFNGGFNLTPKWKLTGSGSYDLETMKLQYITMSINRDMH
                     CWQLSINVIPVGYTRSFNFSISPKSTLLQDLKINRTRYFNTY"
     gene            complement(7743..8483)
                     /locus_tag="PLU11_05150"
     CDS             complement(7743..8483)
                     /locus_tag="PLU11_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPN58533.1"
                     /translation="MTLIKNIAGKLLAAWAILSFIATFLIIFIPSQLTWLIPDPKGQD
                     LFIRISRGWMKVWLLMVGCRYQIRGKENFKKGESYIVTCNHNSLLDVPLSSPFIPGPN
                     KTIAKSSFAKVPLFGFFYMKGAVLVNRKNDRSRRESYEKMKNVLKGKMHMCIYPEGTR
                     NRSDEPLKKFHDGAFRLSVDTGYAILPAVIFNTKKAVPVNKGFYFRPTKIGIHFLPPV
                     NPAGKTTEQLREEIFRITHDYYVKGIDQ"
     gene            complement(8505..9179)
                     /gene="ung"
                     /locus_tag="PLU11_05155"
     CDS             complement(8505..9179)
                     /gene="ung"
                     /locus_tag="PLU11_05155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPN58534.1"
                     /translation="MDVKIEASWKSALKAVFSQPYFEQAALHVRTEKGQGKTIYPPGS
                     MIFNAFNHTPFNKVKVVILGQDPYHGAGQAHGLCFSVQPGIAPPPSLVNIYKEMQADL
                     GIPIPPHGTLTHWAEQGVFLLNASLTVRAGEPMSHAKIGWAPFTDAVIKTISDKKENV
                     VFLLWGKFAKEKSVLINQAKHLILRAAHPSPLSAHAGFMGCKHFSKTNDYLVKHGIDP
                     IDWRLD"
     gene            9243..9316
                     /locus_tag="PLU11_05160"
     tRNA            9243..9316
                     /locus_tag="PLU11_05160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9277..9279,aa:Arg,seq:tct)
     gene            complement(9870..>12000)
                     /locus_tag="PLU11_05165"
     CDS             complement(9870..>12000)
                     /locus_tag="PLU11_05165"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HPN58535.1"
                     /translation="IPTTTYAQCNVNEKYDKVVSGYHQYIALKNDGTFAVWGQDLNNA
                     GTGDLLTPVNITVANFAALTGTPLKATIGGAGGSGKEQAILLTSTGLFAWGTEGYVVA
                     NSLTSNAAFQKIGTPTGGDGTTKLPSGVAPADVLQMTASYQTLILLTTGGNVWILTQS
                     SANMQGNGAAAATTTWHQVKKNASTVLSGVTAVRGQVSNSTYGAVMALCSNGEVYAWG
                     NSVYLGNGTNSAAKNYATLMTLPAEFTSSNIPKMIGVTGGVKGTSTVKNTFYLVSNLG
                     SLYALGDNTKKQCGTFNTTEQKSWVKAKVNSTTEFNNISYISVQEHDASYPGVAAITT
                     TGNLYTWGENDGMMLGRTTDGSTYDPGFPGGFTSGTDKAVSVEVGGHTLVYLREGSAQ
                     FCYVGHKVGGSMGDNTGTDTNVNTFNCSGTPNISICGYVPVAASAITSAISVAQSLIT
                     ANGISTTTITIRLKDASGTNLTTSGGTITLTSSAGTLGTVVDNNDGTYTVILTSTSSP
                     ATALIGFAINGTTASNTTSVVFANTLPLKWGDAKAFRKYKTQQLEWITEQETMVSHFE
                     VERSINGRDWSVILAHKNATNGDLMHTYTHTDNEYIPGVVYYRIKEVDKDGKSYYSAV
                     LMVEADAGFNRIIAYPNPADKLLYIGNVEKSKLSNIQLFSINGEKIKEWNQPQISYDV
                     SNIPTGIYLLKVNTTDGAQQTIRIKKI"
BASE COUNT         3006 a         2852 c         2730 g         3412 t
ORIGIN      
        1 cctgtataaa atagatacat tccgcatagc cggccgctct tacagaatcc agcgaacctt
       61 ccttgaacca gccatccatc cggattgacc gtacctggtt gtaaatgccc ggttcggatt
      121 cattaaccag gaaactgttc tcaaacactt ttacccggtc agcttttttg tttttagtaa
      181 acaacagcac tgtatcgccg gtgatctggc tgcctttgga ccagagtaca ggattttgaa
      241 acaagcggaa tacggaatct ttaaaggaat aaaacatact gtcactcaca gcctgcatgg
      301 aatccgaaaa gatccgcaca ttccggtagg cttcaaaata acggtcggta ctgtcttttt
      361 tattcggatc cttttttgcg agtgataggg agtctgtagc cgtacgtttt gcaagagaat
      421 cgctgctggc tgcaggtgta agttgaacag cgggcattcc ctttttgctg ctatcagcaa
      481 ccggaggaag ttgatccgct gctacaatta ccggggccac cacactgtct tttgtcaatg
      541 aatcctgcct ggatgctatc aatacgagtt cgtcttcctt tttggaaagg gaatctgtgg
      601 ccggtgctat gggctgaggg gccgtcagac tgtctccctt attggcccct ttattttttc
      661 caagattaat cacttgcacg cctttcatgg tatcctttac catcgaatct ttccgggcgt
      721 acaggtcact cagccgggcc gaaaacaagg tgtctgccgt tacataaatg gagtcattgt
      781 cctgtttgat gatcatcagc ggcttgttgg tggccagcat ccggtcattt tttttatcct
      841 gccacaccac accggccaat atcgtagttc cctgaaccga atcaattatc accacattcc
      901 ctagtgcctg agaggtctgc aggctgtcat taaatgcaaa ctggtcaccc gtaatataaa
      961 cccttgattt ggtatcccgg atcaccggcc gtttcccgaa ttcggcttta cccgttgcca
     1021 tattgtaaaa gccatcggag gtttcaatga tacggccact ggtatctttt atgacggtct
     1081 gggcaataaa gcgggtagac tgactttgtg tattatacaa taaagagtct gtataaatat
     1141 catatgccgg gtccttcatg accacattct tcttaaaata gatatcccgc agaccggcat
     1201 agtaccaacc ttcctggctg gtgagtactg attttttatt caccacccgg cccccgtttt
     1261 tataaatacc tatttcggta ttcatatcat attccaggtc gggtgtggtt aatgttcctt
     1321 tgccatcggt gagtttcacc ccgccatcga ggtaagcaat ttttttatca atcaggtaac
     1381 gcaggtgact ggaggtaata ttaaccgtat ccacatcctt gatatgcaca ttgccccagg
     1441 cttcaaaaat ccgtatccca ttattcagca cacagctgtc gcaattaaat aagctggatc
     1501 cctgtttgag ccgaacatgc ccggccagga tctgcagggt ggtactgtca tccactttca
     1561 gaatggtaaa ccggtcggag cccaaaatct ggatatcccg gagggaatcc gatgttgcct
     1621 gtggattaat aggagtggcc gccggttctt ttacctgtgc attcatcatt gcaggaaaga
     1681 gacaggtgcc tgtaaacaat attccgaaaa ataaaaggat gcttcgcatt agttagctgc
     1741 cgcggcagta tcttttttcg caggcgaagt taatggcccg gttttatctt tcttgccaaa
     1801 gatggaaaga ttaacgtagc gtttaggatg cgtacgcaga tcatcgatca gaatttccgc
     1861 actcaggatc gcattattta acttatcata caaagcccgg tcattcatca atgcacccag
     1921 cgtaccatcg gaactgttca gtttggccgc tgtggctttt agtgaactga tcatggtatt
     1981 cagtgaatca attgtgggct gcaattcaag ggccgccagt ttctcactta cttttttagc
     2041 attcccgatt gtagccgtaa tatcctcact gttattttta agactttcgg tcatggcctg
     2101 ggcattgttc agggttttgg caagtgctcc gttcttatta tcgagcaggc cattcagggc
     2161 attactggcc tgacttaagt tcgccaatgt ttctttcagg taagctttat tctccatatt
     2221 gagcatacca ttcactccac taagcgtttc cttcagtgaa tccagtacat cccttatccg
     2281 ggccatggtg ggattcattt gtgcttttac atcccccagg ataccggttt ccatccgggt
     2341 actgagtgta tcgccatctc ccatcatttc agtggagttg cctctttcaa ttacgatata
     2401 ggaagatccg acgaggctgg aggatatata agccaccgag ttttttggaa tattgatatc
     2461 gcgggtgaga ctgatcgtca ccaccacagc actcacatcc ttatcccgtt cttttttatc
     2521 atacacatta ccgatcggga gaccgttaat cttagcttca tttgattttt ccagggtgcc
     2581 cagttctttg aaaacggcat agatcttttt gctgcggctg aacacatcct tgcccttcag
     2641 gaaattgaac ccaacgatca ggatcacaag ggcgacaatg gttaatattc caactttggt
     2701 ttcgttgctg attttcatgc tgctgcgata aggctgatta aagccattcc gggggccaaa
     2761 ttagctaatt atttttaaac agtcccgggc ttagaatcct ttggtggctt cttccgttac
     2821 ttcagccgcc ggttttgccc ttatttcaag cttttccttg tagtttttga tggccgtgaa
     2881 gatattggcc acaatttcag cctggccgat ctcactgttc atatattctt cctcttcctg
     2941 gttggagata aacccgattt cgatcaggat actgggcatt ccggtagcct gcaataccca
     3001 gattccttta tcattccgtt gttttactcc ccggttgacc cggccggcgg caataaattc
     3061 ctgttgtacc aggtcggcca gattgaggct tttccggaaa taattttgtg catagataag
     3121 catccgtgct ttttctgacg gatcagaagg gtcaggtaac gacagggtgg tcgtagagtc
     3181 aatttcaccg gtataatcgg tggtcttatt caccgacctg actttatcat catttttatt
     3241 catcgcccag acataggtct ctgtcccttt ggccttattg ggcacccata cattttcata
     3301 ttcgtattcg taataggtct tcttataggt tttcttgcct ttcttcaccg ttttcgtacg
     3361 gggaatctta ttgaccaccc gtcgttcata ccagccaccc ggcttgtgtc cggctgcatt
     3421 taagtgtatg gcaataaaca aatcaccccc tgattcgttg gccagatcag ccctgtattt
     3481 cagggcggca tctttcgtgg gcttatttcc aggtaataca tccgtagtac gggtgaaaac
     3541 cagcttcaca tcgggccagg ctttggcaat agtatccccc agtttcagcg atacctgaag
     3601 ggctaactgc gcttcggttg acttcatacc gggagccccg ggatcgatac cgccatgacc
     3661 cgggtcgatg ataatggttt gtatcggttt tttcctgtcc tgggcacata aatcagtcgt
     3721 ccacacgaag caggtgagca gggtaaaggc gatacatact tttttcattc agttcctgtt
     3781 taatgattgt tcttctgatt aaactttcag cggaccttgc tgcattccat ttcctctata
     3841 cggttcagca tggcggatac attccgggta tcataccggc tgtttccact gttatcctgg
     3901 tcattggtct gctgttgttt ttccagccat gcaatataac cagaaaccgc ccgaatgata
     3961 catccggcca gttctttttg tccctctttg ctattcagat aatcctcttc cgcccggttg
     4021 gtgatatagc cggtctccac cagtatactg ggcatggcgg tggcttgtaa aacccatatg
     4081 cccacacccc gttgccggac atcccgatcg acccggccta ctcttgaaaa ctcatcctgt
     4141 accagtccgg ccagggtagc gcttctccgg aaaaactgtt ttgtttttaa cagggaaagc
     4201 gcaatgaatt cgggcgaatt gggatccacc tccccgtaat ttgctttata attatcttcc
     4261 atcagcatcg gggcgttttc ccgcatcgct atttccttat cctcattttt atgggaaccc
     4321 cagatataag tctccgtacc cttggccggg ttgggttgac tatgggtacg atactgggga
     4381 actttcttcg tttgtttttt ccggttcttg cccttgccgg tgtagtaggt taccgtttta
     4441 tgtccgatca gctcagaacg tttgatcgga agggctgcat tcacgtgtat gcaaataaat
     4501 aaatcgcctt tattttcatt agcaatctct gctctgcggt gaatcggcgt gattttatct
     4561 gttggcctgg attccacaat ccggatttca ggcatctccg catggaattt tttgaccagt
     4621 tctttggata cagccaggca gatttcatct tcataggaat aggaaccctt cgccccgtta
     4681 tgaccgccat tggccataat tccatgcccg gcatccacaa tgatcgtttt aataactttc
     4741 tgcttcttta ccgcactggc aggtgaaccg gaacggaagg agtaaaaact gatggaaata
     4801 aaaagaaata gaaaggccag gaaaaagcgt ttgatcatag tttttcattt tgcgtccggt
     4861 ggacttttca tcacagggtc agcataagtt tcacaaattg tttaggccaa tcaataggta
     4921 gccgggtgag aatgaattac atttgccccg ttgatgacgc ctcaatacaa atttaggtca
     4981 aaaaccattt cttacgggat actgactgcc gttataattt actcacaaac gtggataaca
     5041 gcagctcgca gtgtgccgca tcattatttt gggactcctt taacaatcaa cccggataca
     5101 accaaacctc cggtcttaaa agaaacgcca caacctgtca ctaccaatac tgataccact
     5161 aaacgaccgg ttacagatac caaccaggta aacgggacag ataccatacc cgtccgtggt
     5221 accgataccg gatatgtgca aaagacggat actttcaacc ttaagttgtc taaggactcc
     5281 cttgaagccc cgttaaatta ttccgcggag gattcggtgg tggtgatgat taaatccaaa
     5341 aagatatatc tctacggcaa aacaaaaacc gagtataccg atatcactct tacagcgcct
     5401 aaagtggaaa tggatcaggc cacccaggtg gtgaccgctt acaaccggaa agacagtacc
     5461 ggcaaagtac tggagaccgc caaatttaaa cagggggaga atgaattcag cagtgatacg
     5521 atccgctata atttcaaaac acaggtggga ctgaccaaaa acaccatgat tccgcaggga
     5581 gagatcctga tcctgggtga atccatcaaa aaagtaaatg agaatacttc atttattaaa
     5641 cgtgcccgct ttaccacctg tatgctggac gatccgcatt ttgcctttgt gaccccgaag
     5701 atgaaagtga tcaaccagaa actggcgatc tccggaccgg cccacccgga atttgaggga
     5761 gtacctgttc cgatatacgt cccatttggt ttttacccgc tgagccaggg acgacactcc
     5821 ggattactcc ggcccaattt tataaccgat gaagtacggg gtatcgggat gcagggactg
     5881 ggctattaca aagtgctgaa tgattactgg gatgctgaag tatcaggtga ccttttttcc
     5941 tacggggaat gggccgcggg tctgaaaatg acctatcgga aaatttataa atacagtggc
     6001 ggtttcaacc tcaccctgcg ctcctccaaa caaaatttta aaggcgatcc tgatttttcc
     6061 aagacccgta tttttaatgt gacctggaac cacagcatgg acagccgtgc ccgtcccggt
     6121 gtcagttttg cggctaatgt aaatgccggt tccaccaagt tcaatcaact ggtgccaggg
     6181 aatgcctacc tgaattacca gaacctgatg ggctcctcca ttacgtttaa taaatcatgg
     6241 aagaataaac cgttcaactt atccatcagt gccacacact cgcagaacaa taacctgggt
     6301 acggtcaacc tgaatctgcc caatgccgct ttcaccgtta gtactttata tccctttcag
     6361 aataagaacc gggccggcac cgccaaatgg tatgagaacc tggggatcgc ttataacggg
     6421 tcttttatga actcagtcag ttttaatgat accatcaaat acgggcaaaa cggggtaaag
     6481 ccattctttc agtttttact ggatactgct caatggtcgg cccagcataa tatcccgatc
     6541 acactttcct taccgccgat actgggaggt aaggtactgg tatcaccggg aatcagttac
     6601 agccagacct ggattcaacg aactaccaac taccaatgga acagtgcctt gaaaaaagtg
     6661 gatacgatta cggccaaagg aattttcatt gaccagcgga gttccatttc actcagcttc
     6721 aataccgctt tatttggtac ggttcaattc aaaaaatcca gggtaatcgc cctccggcat
     6781 accgtgcgtc cccagatagg aatgagttat accccggacc tcaacacttc gcatattaag
     6841 aaaacccagg tggataccac ggggaaagtg ctttattata atgaaatcgg cggaaccgta
     6901 ttatacaata ccagttccag aagaagcggg gccatgagtt ttggggtgga taataacatt
     6961 gaaatgaaag tccgttccaa aaaagatacc ggcgacctgg ccattaaacg cgtgaaactg
     7021 atcgagggtt ttgggtttag cggctcctat gattttatcc gcgattcgtt caggttatca
     7081 cctatcagtt tttatttccg tactacttta tttgaaaagc ttaatatcaa tgccagtacc
     7141 accctgaatc cttatcagac cgattccagg ggatatgata ttgcgaaata tgcctggcag
     7201 ggaggtaagt ttagtccagg aagactgact tccggtagtg tatccatgag caccaatttc
     7261 cagagtaagc ccaaggatgc caagaaagat gaagcccgta aaaaagcggt ggaagaacgg
     7321 ctgcaggatc cggctttgct gggtgatcag caacgtttac tggaatttat gcaacagaat
     7381 ccgtcgcagt ttgtggattt taatatcgac tggaacctgg gattaagtta ttcactgtcc
     7441 tttgttaacc gcatcaaatc tgatttcagc gggtatgaaa ccgacttcca gttgggaggt
     7501 gtttctttta acggggggtt caacctgacg cccaaatgga aactgaccgg tagtggaagc
     7561 tatgacctgg agaccatgaa actgcaatat ataacgatgt cgatcaaccg cgatatgcac
     7621 tgctggcaat tatccatcaa tgtaattccg gtgggatata cccgctcctt taatttctcg
     7681 atcagtccga agtccacttt actgcaggac ctgaaaatca accggacccg ttatttcaat
     7741 acttactgat caattccctt tacatagtag tcatgcgtga tgcggaaaat ttcttcccgt
     7801 aattgctcgg ttgtttttcc ggccggattg accgggggca ggaaatgaat cccgattttt
     7861 gtggggcgga agtaaaaacc tttattgaca ggcacagcct ttttggtatt aaatataaca
     7921 gctggcagga tagcatatcc ggtatccaca gacagccgga atgcgccatc gtggaatttt
     7981 ttcaggggtt catcactgcg gttacgggtg ccttcgggat aaatgcacat atgcatcttg
     8041 ccttttaata cgtttttcat tttctcataa ctctcgcggc ggctgcggtc atttttccgg
     8101 ttcaccagta cggccccttt catatagaaa aatccaaaca gcggtacttt ggcgaaggag
     8161 gatttggcaa tggttttatt cggacccggg atgaaagggg aagaaagggg tacgtccagc
     8221 aatgaattat gattgcaggt aacgatgtag gattctcctt ttttgaaatt ttctttaccc
     8281 cggatctggt accggcagcc caccatcagc aaccatactt tcatccatcc cctggagatg
     8341 cggataaaca gatcctgtcc ttttggatcc ggaatcagcc aggtaagctg ggaggggata
     8401 aagatgatca ggaaagtggc gataaaactg aggatggccc aggccgccaa cagcttaccg
     8461 gcgatatttt ttataagcgt catacgtagt tggtttttgc cggtttaatc caggcgccag
     8521 tcaataggat caatgccatg ttttaccagg tagtcgtttg ttttggaaaa atgtttacag
     8581 cccatgaatc cggcatgtgc cgaaagcggg gaggggtgtg ccgcccgcag gatcaggtgc
     8641 ttcgcctggt tgatcagcac cgatttttcc ttggcaaatt tcccccataa aaggaaaacc
     8701 acattttctt ttttatcaga aatggttttg atcactgcat cggtaaaggg ggcccagccg
     8761 attttggcat gactcatggg ttcgcccgcg cgtaccgtca gggaggcatt cagtaaaaaa
     8821 acgccctgtt ccgcccaatg ggttagtgtg ccatggggag gaatagggat acccaggtct
     8881 gcctgcattt ctttataaat attcaccagg gatgggggcg gggcaatacc cggttgaacg
     8941 gagaagcaga ggccatgtgc ctggccggct ccgtggtagg ggtcctgtcc caggatcacc
     9001 acttttactt tgttgaacgg agtgtgatta aaagcgttaa aaatcatgct gccgggcggg
     9061 taaatagtct tgccctggcc cttttccgta cggacatgga gagccgcctg ttcgaaataa
     9121 ggctgactga ataccgcttt aagtgctgat ttccaggagg cttcgatctt tacatccatg
     9181 cgtttttcct ttggctgcaa gctaaaaaaa aattaccttc gccgctcgtt cttttgaaat
     9241 ttggtccggt agctcagttg aatagagcaa ctgccttcta agcagtaggt cattggtttg
     9301 aatccaatcc ggatcacagg aagtctgcct ccaaagggca ggcttttttt ttgcctgcgg
     9361 caaatagggt ggccacgaaa acacacaaag ggccacgaat aaatacaaaa ggcaggccac
     9421 ggatagcacc gatatacacg gatttttttg cctgcggcaa atagggtggc cacgaaatac
     9481 acgaatttgc cacgcctgtc ccgccaaatg cgggaaataa tacagaaaga caagtataat
     9541 atcccgctaa gaataaaaga gtgtccggat atggaagtgt tttcaacttt cggaaagtct
     9601 taaaagcttt gcgggataaa tttttgaaag gctcattaaa gaaacggata cagaattttt
     9661 taccagatca tatcaggagg actgccgact gctatctgcg aactgccgac tgctatctgc
     9721 gaactgccga ctgctatctg cgaactgccg gctgctgtat ctctataata gaaaaaccac
     9781 ctattcataa gtggtctttc taaagcagcc agaagggttc catcctatct tattgccata
     9841 cggacatcag tgtcctgaca aatcgtttgt tatatttttt taatccggat tgtttgctgg
     9901 gcgccgtcag tcgtatttac tttcaggagg taaatgccgg tagggatgtt gctgacatca
     9961 taggaaatct gcggctgatt ccattctttg attttttctc cgttgataga gaataattga
    10021 atattactga gttttgattt ctccacatta ccaatataca atagtttgtc ggccggattg
    10081 gggtaggcaa tgatccggtt aaatccggca tctgcttcca ccatcaatac cgcagagtag
    10141 tatgatttac catctttatc tacttctttg atgcggtaat aaaccacccc cgggatatat
    10201 tcattatctg tatgtgtata ggtatgcata agatcaccat tggtggcatt tttatgggca
    10261 agaataactg accagtcacg gccgttgata cttctttcca cttcaaaatg gctgaccatg
    10321 gtttcctgtt cggtgatcca ttccagttgt tgggttttgt atttgcggaa ggcttttgca
    10381 tcgccccatt tcaacggaag ggtgttggca aaaacgacgc ttgtggtgtt actggccgtt
    10441 gtgccattga tcgcgaaacc aatcagagcg gtggccgggc tggaagtact ggtaagaata
    10501 acagtatagg ttccatcatt gttatcgact acggttccca gtgttccggc tgagctggtg
    10561 agggtgattg tgcctccact ggtcgttaaa ttagttccac tggcatcttt taaccggatc
    10621 gtaatggtgg tcgtactgat accgtttgca gtaatcagag actgtgccac gctgatagcc
    10681 gaagtgatgg cactggcggc aacgggtaca taaccacaga tactgatatt gggtgtaccg
    10741 gaacaattaa atgtatttac attggtatcg gtaccggtat tgtcgcccat actgccgccc
    10801 actttatgac cgacataaca gaactgggca ctgccttccc gcaggtatac cagggtgtga
    10861 cctcctactt ctactgagac cgctttatcg gtgccggaag taaaacctcc aggaaaacca
    10921 ggatcatagg tacttccatc agtggttctg cccagcatca ttccgtcgtt ttctccccag
    10981 gtatacaggt tgcctgttgt ggtaatggct gcaacacctg gatatgaagc atcgtgctcc
    11041 tgcaccgaga tataacttat attattgaac tcagttgtgc tgttcacttt agcttttacc
    11101 cagcttttct gttccgtcgt gttaaatgtg ccacattgct tcttagtatt gtcacccaat
    11161 gcatataaac tacccaggtt actgaccagg taaaatgtat tcttcaccgt acttgtcccc
    11221 ttcaccccac cggttacccc gatcattttt ggaatattag aagaagtgaa ctcagccggt
    11281 aaagtcatca gtgttgcata gtttttggca gctgagttgg tgccatttcc caggtaaact
    11341 gaattacccc aggcatagac ttctccattt gaacaaagag ccataactgc accataggtg
    11401 ctgttgctga cttgtccacg gacggccgta accccggata ataccgtact ggcatttttc
    11461 ttaacctggt gccaggtagt ggttgcagcc gcagccccat tgccttgcat attcgcgctg
    11521 gattgggtta atatccaaac attgcctccg gtagtcagta agatcagcgt ctgataggag
    11581 gcagtcattt gtaaaacatc cgcaggtgcg acaccgcttg gtaacttagt agtgccatcg
    11641 cctccggttg gtgttccgat tttttgaaaa gccgcattac tggtcagact gttggctact
    11701 acatatcctt ccgttcccca ggcaaaaagc ccggtactgg taagcaggat ggcttgttcc
    11761 ttcccactgc cgcctgcacc accaatggta gctttgagtg gcgtaccggt cagtgcggca
    11821 aagttggcaa cagtaatatt caccggagtg agcaggtcac cggttcctgc attattcaga
    11881 tcctgacccc agactgcaaa agtgccgtca ttttttaatg cgatatactg gtgatagcca
    11941 ctgaccactt tgtcatattt ttcattgaca ttacattgtg cgtatgtcgt ggttggaata
//
LOCUS       DAOPMW010000064        11914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5656_length_11914_cov_10.312084, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000064 DAOPMW010000000
VERSION     DAOPMW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_11914_cov_10.312084"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLU11_05170"
     CDS             complement(<1..1141)
                     /locus_tag="PLU11_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN58536.1"
                     /translation="MKYGFCLLICLPLLISAQESGKPFKLNGTLKDLKFQPEMVYLQY
                     QANGEWKTDSTVVNENKYTFSGQLNEPSQARLRVKYKAGENGVKVPMSGPRDIATVFI
                     QPGKIKVSAVDSFSNISVKGSAVHKAYAVLREQEKPFEERMKPLIEKYREYGKAKDLA
                     NQNKVEEEIDAIDKEKNEKIYGDYVKANPASPLALWALKQYAGWDINADKVEPLYQSL
                     SQKNKDYPSSADFREALEIAKTTGIGKFAPDFTQNDTLDQPVSLSSLRGRYVLVDFWA
                     SWCGPCRAENPNLVKVFNKYKDRNFHIIGVSLDRAGAKEKWMKAIHDDQLTWTQVSDL
                     KFWDNAVAKQYGIKAIPQNLLLDPEGKIIAKNIRGEELEKKLAEVM"
     gene            1349..2914
                     /gene="rny"
                     /locus_tag="PLU11_05175"
     CDS             1349..2914
                     /gene="rny"
                     /locus_tag="PLU11_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPN58537.1"
                     /translation="MDPNIIPVIIGAVGLGLGLLLGKFIFAKNTRKQIEEAELQSQTI
                     LKEAELRAETIRKEKELEAKEKAVQLKAAHDKEVFEKNKKINDSENRIRQKEQTINQK
                     EGNLDKQIKENEAIKENLNRQIELINIKRTELEKHQEEHIRRLEKIANLSADDARNQL
                     IESLKHEAQSQAMVLQQEIIEDAKLKANKEARKIIIQTIQRTAAEQAIENSITVFNLE
                     SDEIKGQIIGREGRNIRAIEAATGVDLIVDDTPEAILLSSFDPLRREIARLSLQRLVA
                     DGRIHPARIEEVVEKTKKQIEEQVMEIGERTVIELGIHGLHKELVRMVGRMRFRSSYG
                     QNLLMHSRETANLCATMAAELGLNPKLAKRAGLLHDIGKVPDEESELSHALLGAKLAE
                     KYGENPAVVNAIGAHHDEMEMQYVISPIIQACDAISGARPGARREIMQQYIQRIKDLE
                     NLAMAYTGVEKAYAIQAGRELRVIVEADKVTDQDSDRLSFEIAQKIQTEMTFPGQIKV
                     TVIREKRAVNVAR"
     gene            3097..3477
                     /gene="rplS"
                     /locus_tag="PLU11_05180"
     CDS             3097..3477
                     /gene="rplS"
                     /locus_tag="PLU11_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPN58538.1"
                     /translation="MNAIAYVHEQLSGKKEFPAFKAGDNITVNYKIIEGNKERIQSFK
                     GDVIKKQGTGHTASFTVRKISDGVGVERVFPFYSPNIESIEINKTGHVRRAKLFYQRS
                     RSGKSARIREKRMAVAVSAAKAKA"
     gene            3609..3818
                     /gene="tatA"
                     /locus_tag="PLU11_05185"
     CDS             3609..3818
                     /gene="tatA"
                     /locus_tag="PLU11_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPN58539.1"
                     /translation="MIQPTLLGMLGTNEIIIILVIVLLLFGGRKIPELMRGLGKGVRE
                     FNDAKSNVKREIEESATDVKNSVKE"
     gene            3859..5283
                     /gene="gatA"
                     /locus_tag="PLU11_05190"
     CDS             3859..5283
                     /gene="gatA"
                     /locus_tag="PLU11_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPN58540.1"
                     /translation="MFEYSSIEQYHARLQEAAVTCVQAVEHYLLQIESCRHLNAFTEV
                     YAEEALAKARLLDQQRLGGQPMGRLHGVVIGLKDVICYKDHTLTAASGILNGFKSVYS
                     ATAVERLLREEAIIIGNLNCDEFAMGSTNENSVYGKVLNARDESRVPGGSSGGSAVAV
                     QAGLCMLSLGSDTGGSVRQPADFCGIIGLKPTYGRISRYGLIAYASSFDQIGIFGTTI
                     PDVALLLEIMAGADEYDSTVSHQPVPAYNKELTPDKKYRIAWFEEALNHPSLDPEIAA
                     GIHGELKKMEAAGHTVKAEKFDLLDYIVPAYYVLTTAEASSNLSRYDGIKFGHRTSVK
                     EADLTDFYKLTRSEGFGKEVKRRIMLGTFVLSAGYFDAYFTRAQQVRQFLCQKMEALF
                     KSYDFIIMPTSPVTAFRAGEKTEDPIAMYLADIYTVMANLVGCPAISIPLFTHSNGMP
                     FGLQLMANRFDEVSLLRFSNELHN"
     gene            5319..6362
                     /locus_tag="PLU11_05195"
     CDS             5319..6362
                     /locus_tag="PLU11_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPN58541.1"
                     /translation="MQFKLPISFFIIFCLYISTATAQRITPDSSGRKDTTAPVISNLE
                     ALKGFQQLFDSKNVGGVTGTRLNPLAVSFVQSYMAKYGKGLRDMRKWAKPYFDLIDNI
                     LTQHGLPKELKYLAVIESGLRYNAISWAGAVGPWAFMPAAGRQYGLIVMSGYDERLDY
                     YKSTHAAARMLTSLYLKYQDWLLVVAAYNCGPGNVDKAIRRSGSRDFWKLQYYLPTES
                     MNHVKKFISTHYVFEGEGGITTVTKEETRDLILKKISTLSEEELKNSTAYKITGRYNS
                     AIILKYIQMEPLAFNKYNPRFDDSIAVNGTYELRLPTQHMNTFIAKRYDILEESLKEI
                     LAPKGTESAVSRQ"
     gene            complement(6364..7596)
                     /locus_tag="PLU11_05200"
     CDS             complement(6364..7596)
                     /locus_tag="PLU11_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPN58542.1"
                     /translation="MLNWVQPFSTFCLLDNHQYQVAPHAAECLVAAGARRVLSAEAGQ
                     AFEALQLFQQEKTSWLFGHLGYELKNESASLNSAHPDRIGFPDLYFFEPETVLRLNEA
                     QLVIEADDPAAIYQAIKSAPDHSHQKRNGPLEIKQCFSREEYLDVIHALQRHITRGDC
                     YEINFCQEFFADNAGIDPLEIYKALSAVSPNPFSAFYRLQDRWLICASPERFLKKTGG
                     QLLSQPIKGTSRRVPGNKALDELNREALFNSAKDRSENVMVVDLVRNDLSRVCKEGTV
                     RVDELYGIYSFPQVHQMISTVSGELKEAVGLSQILQATFPMGSMTGAPKKRVMELIEQ
                     YERTRRGIFSGALGYIDPQGDFDFNVVIRSIMYNQEEDCISFQAGSGITHYSVAADEW
                     EECRLKAAAIIKVLEKVS"
     gene            complement(7686..8129)
                     /locus_tag="PLU11_05205"
     CDS             complement(7686..8129)
                     /locus_tag="PLU11_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58543.1"
                     /translation="MAIKPAKEKKSTAMAGAASSVEAAKKAAPKPSKKEDDEEEDDDF
                     DEDAEVKPAKKAGKATTKKGKDEDDEEEEDDVEETDDWDKPEEEEEEWDPDFDEFDIP
                     KSKGKKAAPAAPGKKAEEEDFKFEDDEFKDLFDDKDYDDDEDDDY"
     gene            complement(8247..9512)
                     /locus_tag="PLU11_05210"
     CDS             complement(8247..9512)
                     /locus_tag="PLU11_05210"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPN58544.1"
                     /translation="MVDVLLGLQWGDEGKGKIVDFFAKDYDMVARFQGGPNAGHTLYV
                     QDKKVVLHQIPSGVFHPGIINLIGNGVVLDPVTLKKECDTVASFGIDLKKNLFISKRT
                     HLILPTHRALDKAAELSKGNQKIGSTLKGIGPTYMDKTGRNGLRVGDLLDKNFTTQYI
                     KLRMKHQRLLDNYNFQEDITAWEEEFFEALEFLKQLNIVNGEYFINQQLKEGKRILAE
                     GAQGSMLDIDFGTFPYVTSSNTISAGVCNGLGVAPQHIRDVIGVTKAYCTRVGGGPFP
                     TELENEMGEELRRIGSEFGATTGRPRRCGWIDLVALNFACMINGVTKIVMTKADVLDA
                     FKELEVCTAYEINGTVTKEVPFQMDRQKPVPVYQQFAGWDTPSSQATKAEEIPATMKT
                     YVDFINAYLGVDIHYISNGPGREQIIKVK"
     gene            complement(9674..>11914)
                     /locus_tag="PLU11_05215"
     CDS             complement(9674..>11914)
                     /locus_tag="PLU11_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN58545.1"
                     /translation="VGKNNAVWTENNSGYKFIPSGGASRFVRSEMFTMGGTLVSTADS
                     STTVAGLLDLSFPNVCVNTGSTQFVVRTTYSVCDGSGGQLVSMDTVTVNLNQMQAAIA
                     TAATSCNGAGNGSALVTPSTTNGPFTYVLDGGTPFTAPGPYNFTNLTSGNHSVTVQDA
                     FGCFTPLLPFTIAAGPPIATTATKTDALCNGGSTGSITVAQPAAGNAPYEYSLDGITW
                     QTSNVFNGLPAGTYTVFFRERDGCSGTLSITVAEPTVLSSTAGAIPVVCNGQSTGTIT
                     ASATGGVSPYEYSINGLNWQGSNVFNVPAGIYTVQIRDVNGCISTQPVTVTEPAALTA
                     TSVNTNATCNGGLDGIILITAAGGNTGYTYSIDGTSFQSSNQFNVAPGNYTVTVRDNL
                     GCTTSFPAVVGLTDDMTWIPQTDPTICESKSVQLELTSNATQYVWSPATGLSNTTIYN
                     PVANPVVTTEYIVTATLGRCTHNDTVIVNVNKAPIPNAGVDGFICYGQNYQLQATGGT
                     EYVWTPSTYLDNANISNPLSTPSKDIVYTVSIKSDVNGCASLVTDDMRIDVTPPIKVK
                     TYPYDTIGYPGDQFQLLVVPSDSDVINYTWNPTIGLSNPNSTIGQGNINIPNPVVTVG
                     AIGQDVQYQVIASTIAGCRGEGYIKVRVYKGPDIYVPTGFSPNGDGKNDKFTPFPVGM
                     KSYNYFRVFNRWGQLVFSTSRLNDGWDGRFGGVEQGNGVYVWMIEGLTRDNRVISKKG
                     TVALLR"
BASE COUNT         3104 a         2883 c         2792 g         3135 t
ORIGIN      
        1 tcataacttc cgccagcttc ttctccagct cctccccccg tatatttttg gcaataatct
       61 ttccttccgg gtctaacagc aggttctggg gtatggcttt aatgccgtat tgcttagcca
      121 ccgcattgtc ccagaatttc aggtcactca cctgggtcca ggtcagctga tcatcatgaa
      181 ttgctttcat ccatttttcc tttgcgccgg cacgatcaag tgaaaccccg atgatatgga
      241 aattgcggtc tttatacttg ttgaatactt tcaccagatt ggggttttcg gcccggcaag
      301 gtccgcacca gctggcccag aaatcaacaa ggacataacg tccgcgcagg gaggaaaggc
      361 tcactggctg gtccagcgta tcgttttggg taaaatcagg ggcgaactta ccaataccgg
      421 tggttttggc aatttccagg gcctcccgga aatccgctga agaaggatag tccttatttt
      481 tctgagaaag tgactggtaa aggggttcta ctttatccgc attgatatcc caacctgcat
      541 attgcttcag cgcccaaagt gctaacggtg atgccgggtt ggcttttaca taatcaccat
      601 agatcttctc gttcttctcc ttgtcgatcg catcgatctc ctcttccact ttattctgat
      661 tggcgaggtc tttggcttta ccgtattccc ggtatttttc aatcagcggt ttcattcttt
      721 cctcaaaggg cttctcctgt tcccgcagga cagcgtatgc tttatgcaca gccgatcctt
      781 ttacggaaat attactgaag gaatcaacag cagagacctt gatctttccg ggctgaataa
      841 ataccgtggc aatatcgcgt gggccggaca tgggcacttt taccccgttt tccccggcct
      901 tgtatttaac cctcaaacgg gcctgggaag gctcgttcag ctgaccactg aatgtatatt
      961 tattctcatt gactaccgta ctatcagttt tccattcccc gtttgcctgg tactgcaggt
     1021 acaccatttc cggctgaaat ttcaggtctt tcaacgtgcc gtttaattta aatggcttac
     1081 ccgattcctg ggctgaaatg agcaggggca ggcagataag aagacaaaat ccgtatttca
     1141 ttcaagaagt tttagggcct caatttacgg ttttaaggtc tttaaaacgg gttctttgta
     1201 tgaccggcag gtggtttccg gtttacaatt ccagatttcc tgtttttccc ggggttttgt
     1261 atcttcgctg attacatatc cgcctgaatt acaaggcggg tatgcgtgtt aaaaaaccgc
     1321 attgtgcatc ttttaaacaa aaaaacgaat ggatccgaat ataattcccg tcattattgg
     1381 cgcagtgggc cttggactag gcctcctgtt gggtaagttc atctttgcca aaaacacccg
     1441 caaacaaatc gaggaagctg aattacaatc gcaaacaatt ctgaaagaag ccgaactgag
     1501 ggccgaaacg atccgcaagg aaaaagaact ggaagcgaaa gaaaaagcgg tgcagctgaa
     1561 agccgcacat gataaagagg tttttgaaaa aaacaagaag ataaacgatt ccgaaaaccg
     1621 gatccgtcag aaagaacaga cgatcaatca gaaagaaggt aatctcgata agcagatcaa
     1681 ggaaaacgaa gccatcaagg aaaacctgaa ccgtcagatc gaactgatca atatcaaaag
     1741 aacagaactg gaaaaacacc aggaagagca tatccgccgc ctggagaaaa tcgctaacct
     1801 ttctgcagat gatgcccgca atcaactgat cgaaagcctg aaacatgaag cccagtcaca
     1861 ggcaatggtg ctgcagcagg aaatcattga agacgccaaa ctgaaagcca ataaagaagc
     1921 ccgcaagatc attatccaga ctattcagcg tacagcggct gaacaggcca ttgagaattc
     1981 cattacggta ttcaacctgg aaagtgatga gatcaaagga cagatcatcg gtcgtgaagg
     2041 acggaatatc cgcgccatag aagcagctac cggtgttgac ctgattgtgg acgatacccc
     2101 tgaagcgatc ctgttgtcca gtttcgatcc tcttcgccgg gagatcgccc gcctgagctt
     2161 acaacgcctg gtggccgatg gacgtatcca ccccgcccgt attgaggaag tggtggagaa
     2221 aacaaagaaa cagattgaag agcaggtaat ggagatcggg gaaagaaccg tgattgaact
     2281 gggtatccat ggcttgcata aagaactggt aagaatggtt ggacggatgc gtttccgttc
     2341 atcctacgga cagaatctgc tgatgcacag ccgggaaacg gccaatcttt gcgccactat
     2401 ggcggcagaa ctcggactga accctaaact ggcgaaaaga gccggtctgc tccacgatat
     2461 tggaaaagta cctgatgagg aaagtgaact gagccacgca ctgctgggag ccaaacttgc
     2521 cgaaaagtac ggggaaaatc cagcggtggt gaatgccatt ggggcccacc acgatgaaat
     2581 ggaaatgcaa tatgtgatct cgcccattat ccaggcctgt gacgccatca gtggcgcccg
     2641 tccaggtgca cgccgggaga ttatgcagca gtatattcag cggatcaagg acctggaaaa
     2701 cctggccatg gcatatactg gcgttgaaaa agcttacgct attcaggccg gtcgtgaact
     2761 gcgggtaatt gtggaagcgg ataaagtaac cgaccaggat tctgaccggc tttcttttga
     2821 aattgcccag aaaatccaga cggaaatgac cttccccggc cagatcaagg tgaccgtgat
     2881 ccgggaaaaa agagctgtca atgttgccag ataaatattt gaaaattaac aggttgagcg
     2941 tttgaaggga aatgacctta gcaggtttga ttattgaaat ttggtgctta tttggatctt
     3001 ggcatcttgt ttcttgtaat ttttcgcaga taatccccgg ttatttggta tttcccccgc
     3061 atcctcttac ctttgccctc cctaaaaata aggattatga acgctatcgc atatgtacat
     3121 gagcagttgt ctggtaagaa agagtttcct gctttcaaag ctggtgacaa tattaccgtg
     3181 aactacaaaa tcattgaggg taataaagaa aggatccagt ctttcaaggg cgatgtgatc
     3241 aaaaaacagg gtacgggtca tacggccagc tttaccgttc gtaagatatc tgatggagta
     3301 ggtgttgagc gggtattccc cttctattcc cctaatatcg aatcaatcga aatcaacaaa
     3361 accggtcacg tacgtcgcgc caaactgttc tatcagcgta gccgtagcgg taagagtgcc
     3421 cgtatccgcg aaaagagaat ggccgttgcc gtctctgcag ccaaagctaa agcatagtaa
     3481 aaacagcaat atatttccag aaagccctga attaatcggg gcttttttgt gaatgaccgg
     3541 cttttcttca attaatattc ctgttttttt aagttgttac tatctttgaa atctaattaa
     3601 gtcagattat gatacaacct acattactgg gtatgctggg taccaacgaa atcatcatca
     3661 tcctggtgat cgtactcctg ttgttcggag gacgcaaaat tcccgaactg atgcgcggtt
     3721 taggcaaagg cgtgcgtgaa ttcaatgatg ccaagagcaa tgtgaaaagg gaaatcgaag
     3781 aaagcgccac tgacgtgaaa aactctgtaa aagagtaatt cacggtctct tatttaactt
     3841 cccataaagc atttgctttt gtttgaatac tcttccattg agcagtatca tgcccgattg
     3901 caggaagcag cggtgacatg tgtacaggcc gtagaacatt acctccttca gatcgaatcg
     3961 tgccggcacc tgaatgcctt cacggaagtt tatgcggaag aggcactggc caaggcccgg
     4021 ttgctggacc agcaacggct gggtggtcaa ccaatgggcc gtctccacgg ggtagttatc
     4081 ggcctcaagg acgttatctg ttataaagat cataccctca ctgccgcgtc cggtattctc
     4141 aatggtttta aatcagttta ttctgccacc gccgttgaaa gactgctgcg ggaagaagcc
     4201 atcatcattg gcaatctcaa ttgcgatgaa tttgccatgg gttccactaa cgaaaactcg
     4261 gtatatggca aggtgttgaa tgcccgtgat gaaagccggg tacccggagg ttcttcaggt
     4321 ggttccgcgg tggctgttca ggccggtctt tgtatgcttt ccctgggcag tgatacagga
     4381 ggcagcgttc gtcaaccggc tgatttttgt ggtattatcg gtctgaaacc gacctatgga
     4441 cgaatctccc gctatggatt gatcgcctac gcttcttctt ttgatcagat tggtattttt
     4501 ggaacaacta ttccggatgt agccctttta ctggaaatca tggcgggtgc cgatgaatat
     4561 gacagtaccg tttctcacca acccgtaccc gcttacaata aggaattaac gcctgataaa
     4621 aaatacagga ttgcctggtt tgaagaagcc ctgaatcatc ccagcctgga ccctgaaatt
     4681 gcggcgggta tacacggtga acttaaaaaa atggaagccg ccggacatac ggtgaaagcc
     4741 gaaaaatttg acctgctgga ttatattgtg ccggcctatt atgtgctgac cacggctgaa
     4801 gcttcttcca atttatcccg gtatgacggg attaaattcg gacaccggac ttcggtaaaa
     4861 gaagcggatc tgactgattt ttacaaactg acccgctcag aaggatttgg aaaagaagta
     4921 aaacgccgga tcatgctcgg cacttttgtt ctcagtgccg gttattttga tgcctatttt
     4981 acacgggccc agcaggtgag gcagttcctt tgccaaaaaa tggaagcact tttcaaatct
     5041 tatgatttta ttatcatgcc tacatctcct gtaaccgcct tccgggccgg ggagaaaaca
     5101 gaggacccga tagccatgta cctggctgat atctatacag ttatggcaaa tctggtaggt
     5161 tgccctgcca tttccatccc gcttttcacc catagcaatg gcatgccttt cgggctccag
     5221 cttatggcaa accgatttga tgaggtatct ttgctccggt tttcaaatga gctacataac
     5281 tgagaaccgg atttaagcaa aaacctgatc tgtacaccgt gcaattcaaa ttacccatat
     5341 cgttttttat cattttttgc ctgtatataa gtacagcaac tgcccagcga atcacccctg
     5401 actcctccgg caggaaggac actacagcac ccgtaatcag taacctggaa gcgctcaagg
     5461 gatttcagca actctttgat tcaaaaaatg tgggaggggt taccggtaca cggttgaatc
     5521 cgttagcggt gagttttgtt cagagctata tggccaaata tggcaaagga ttacgggata
     5581 tgcgcaaatg ggcgaaaccc tatttcgatc tgattgataa tattctgacc cagcatggtc
     5641 tgcccaaaga actgaaatac ctggcggtga ttgaatccgg gctgcgttat aatgccattt
     5701 cctgggctgg tgcggtaggt ccctgggcat ttatgccagc agcaggtcgt cagtatggcc
     5761 tgattgtaat gtcgggctac gatgaacgcc tcgattatta taaaagtacc catgcggccg
     5821 cacggatgct gaccagctta tacctgaagt accaggattg gttgctggtg gttgccgcct
     5881 ataattgcgg acccggaaat gtagataaag ccatcagacg cagtggcagc cgggatttct
     5941 ggaaactcca gtattatctg cctacggaat ccatgaacca tgtaaagaaa ttcatttcca
     6001 cccactatgt atttgaaggg gaagggggga ttactacggt caccaaggaa gaaacgcggg
     6061 acctgatttt gaaaaaaatt tccacgctga gcgaagagga gctaaaaaac agtacggcct
     6121 ataaaatcac cggccgctat aattccgcca tcatactgaa gtatattcag atggagcccc
     6181 tcgcctttaa taaatataat ccaagatttg acgactccat cgcggtcaat ggcacgtatg
     6241 aactacgtct gcccactcag catatgaata cctttattgc caaaaggtat gatattctgg
     6301 aggagagcct gaaagaaatc ctggccccca aaggaactga aagtgctgtc agccggcaat
     6361 agatcaggat actttttcca acacttttat aatagctgct gcttttagtc tgcactcttc
     6421 ccattcatca gccgccacag aataatgcgt gattcccgat cccgcctgga aggaaataca
     6481 atcctcctcc tgattataca tgatactacg gatcacgaca ttgaaatcaa aatcaccctg
     6541 cggatcgata taacccaacg cgcctgaaaa aataccccgg cgggtacgct catattgttc
     6601 aataagttcc atcacccgtt tcttgggtgc tccggtcata gagcccatcg ggaacgtggc
     6661 ttgcaggatc tgactgagac caaccgcctc tttcagttcg ccacttacgg tcgagatcat
     6721 ctgatgcacc tgtgggaacg agtagatacc atagagttca tctacccgta ccgtcccttc
     6781 tttacagacc cttgaaagat cattgcgaac caggtccact accattacgt tctctgaacg
     6841 atccttggcg ctgttaaaga gcgcttcgcg gttcagctca tcgagtgcct tatttccggg
     6901 aacacgccgg gaagtgcctt taatgggctg tgaaagcagt tgaccgccgg tcttcttcag
     6961 gaaacgttcc ggactggcac aaatcagcca tcggtcctgt agccggtaaa atgctgagaa
     7021 tgggtttggt gacaccgcac tgagcgcctt gtaaatttcc agtggatcaa tccccgcatt
     7081 atcggcgaag aattcctggc aaaaattaat ctcgtagcaa tcgccccggg tgatatgtcg
     7141 ctgcagcgca tgaatgacgt ccaggtattc ttcacgactg aaacactgtt ttatttccaa
     7201 tggcccattg cgcttttgat ggctgtggtc aggcgcagac tttatggcct ggtatatggc
     7261 agccggatca tccgcttcaa tcactaactg agcttcattt aaccggagta ctgtttccgg
     7321 ttcaaaaaag tacagatccg gaaatccaat ccggtccgga tgggcggaat tgagggaggc
     7381 tgattcgttt ttaagttcat acccgagatg gccaaatagc caactcgttt tttcctgctg
     7441 aaaaagctgg agcgcctcaa aagcctgtcc ggcttcagcc gataacaccc gtctggctcc
     7501 ggccgccacc aggcattccg cagcatgcgg agccacctga tactggtgat tatcgagcaa
     7561 acaaaaagtg ctaaagggtt gaacccagtt cagcactttt tgttttatct gcccgaaatc
     7621 acttaccggg aaaacctgac tgttaaagtg atggctattc aatagtgtga tgcgtaaata
     7681 gtcggttaat aatcgtcatc ctcatcatca tcgtagtcct tgtcgtcaaa caggtcttta
     7741 aattcgtcat cctcaaattt gaaatcctct tcttctgctt tcttaccggg ggcagcagga
     7801 gcggctttct ttcctttgga tttgggaata tcgaattcat cgaaatcggg atcccattct
     7861 tcttcctctt cttcgggttt atcccagtca tctgtttcct ctacatcatc ttcctcttcc
     7921 tcatcgtctt cgtccttgcc ttttttagta gtggctttgc cagctttctt tgcaggcttc
     7981 acttccgcgt cttcatcgaa gtcgtcgtcc tcttcttcat cgtcctcttt cttactgggt
     8041 ttgggcgcgg ctttcttggc tgcttcaaca gatgaagccg ctcccgccat tgcggtactc
     8101 tttttttcct ttgctggttt tatagccata gtttaacagg attaatattg taaaatttca
     8161 gcgaagtttt taattagcca aatttttaaa attttttttt ctaaaataac ccccgtaagt
     8221 acaggttggt taatcgttcg gattccctat ttaaccttaa taatctgttc ccgtcccggt
     8281 ccattagaaa tatagtgaat atcaacaccc aggtatgcat tgataaaatc cacataggtt
     8341 ttcatggtgg cgggtatctc ttccgccttg gttgcctgac tgctgggggt atcccaaccg
     8401 gcaaattgct ggtaaaccgg caccggtttc tgacggtcca tctggaaagg tacttctttc
     8461 gtaacggttc cgttgatctc ataagcagta catacctcca gttctttaaa cgcatccagc
     8521 acatccgctt tggtcatcac gatcttagtg accccattga tcatacaggc aaaattaagg
     8581 gccaccaggt caatccagcc acagcggcgg ggacggccgg tggtggcgcc gaattcgctg
     8641 ccgatccggc ggagttcctc gcccatttcg ttttcgagtt cagtagggaa agggccacct
     8701 cccacacggg tgcaatacgc tttcgttacg ccgatcacat cccggatatg ttgcggggcc
     8761 acccctaaac cattgcagac ccctgcagaa atggtattgg aggaagtcac ataagggaaa
     8821 gtgccaaaat cgatatccag catgctgccc tgtgcccctt cggccagaat tcgttttcct
     8881 tctttcagtt gctggttgat aaagtattcg ccgtttacaa tattcagttg ctttaaaaat
     8941 tcaagggctt caaaaaactc ctcttcccag gccgtgatat cttcctggaa attgtaatta
     9001 tcgagcaacc gctggtgttt catccggagc ttgatatact gggtcgtaaa attcttgtcc
     9061 agcaggtccc ctacccttaa accattgcgg cctgttttgt ccatataagt ggggccaatt
     9121 cctttcagtg tggaaccaat cttctggttc ccttttgata attcagccgc cttatccaaa
     9181 gcgcggtgcg tgggaagtat caggtgggta cgtttggaga taaacaggtt cttcttcagg
     9241 tcaataccaa atgaagccac ggtatcacat tcttttttca gggtaaccgg atcaagcacc
     9301 accccatttc cgatcaggtt aatgataccc ggatgaaaga cccccgatgg aatctgatgg
     9361 agtaccactt ttttatcctg cacatacaaa gtatgtcctg cattgggacc accctgaaac
     9421 cgggctacca tatcataatc cttcgcgaaa aaatccacga ttttcccttt tccttcatcg
     9481 ccccactgga ggccaagtaa tacatcaacc atgattcaat ttctgttttt ttaattctgc
     9541 caacaggccg gaaccgggca atcacccggg cgccgcaaaa atacggtatc aagaaaaagc
     9601 caccgaatta aatacggtgg ctgctgaata ttttattccc atccgggagg aacttatcaa
     9661 cttagggaaa cacttagcga agtaaggcta cggttccttt tttggagatc acccggttat
     9721 ccctggtcag gccctctatc atccagacat agaccccatt accttgttcc acgccgccaa
     9781 aacggccatc ccagccgtca ttcagacggg aagtggagaa aaccagctgt ccccagcgat
     9841 taaagaccct gaagtagttg tagctcttca tacccaccgg gaaaggggtg aatttgtcat
     9901 ttttaccgtc cccgttgggc gagaaaccag tcggcacata gatatccggc cctttataca
     9961 ccctgacttt gatataacct tcgcctcgac aacccgcaat cgtggaagcg attacctggt
    10021 attgaacatc ctgaccaatg gcgccaaccg tcactaccgg gttggggata ttgatattac
    10081 cctgccctat tgtactgttg ggattgctga ggccgatggt cgggttccag gtataattga
    10141 tgacatcact gtccgaagga actaccagta actggaactg atcgccgggg taaccaatgg
    10201 tatcatacgg ataggttttt actttgatcg gcggggtgac atcaatccgc atatcatcgg
    10261 ttaccagcga agcacaacca tttacatccg atttgatgga aacagtataa acgatatcct
    10321 ttgaaggagt ggaaagcggg ttggaaatat tggcattatc caggtaagtg gaaggtgtcc
    10381 agacatattc tgtaccacct gttgcctgca actgataatt ctggccataa cagatgaatc
    10441 catccacccc ggcattggga atgggggctt tatttacatt cacgatcacc gtatcgttat
    10501 gcgtacaacg acctaaggta gccgttacaa tatactcagt ggtcactacc gggttcgcta
    10561 ccggattata gatggttgtg ttactgagtc cggtagccgg gctccacaca tactgggtgg
    10621 cattggaggt cagttccagt tgtactgatt tactctcgca gatcgtggga tccgtctgag
    10681 gaatccaggt catgtcatcg gtcagaccaa ccacagccgg aaagctggtt gtacaaccca
    10741 aattgtctct gaccgtaacg gtatagttgc ccggtgctac attaaactga ttggatgatt
    10801 gaaagcttgt tccgtcaatt gaataagtat agccggtatt tccccctgct gcggtaatca
    10861 gtattattcc atccaggcct ccgttacagg tagcattggt atttaccgat gtggccgtca
    10921 gcgcggcggg ttcggttacc gttaccggct gggtgctgat acaaccattc acgtcacgga
    10981 tctgcacagt ataaattccg gccggtacat tgaatacatt gctaccctgc cagtttagtc
    11041 cgttgatcga atattcataa ggcgaaacgc caccagttgc agaagcagtg atggtaccgg
    11101 tactttgccc gttacaaacc accggtatgg ctccggctgt acttgacaga acggtgggtt
    11161 ctgctactgt aatactgagg gtgccactgc aaccatctct ttccctgaaa aaaacagtat
    11221 atgtaccggc aggtagtcca ttgaaaacat tgctggtctg ccaggtaatc ccatcgagcg
    11281 agtattcata tggtgcatta cctgcagcag gctgcgccac cgtgatggag ccggttgagc
    11341 cgccattaca taatgcatcc gtcttggtgg cggtagtggc aattggtggt ccggctgcta
    11401 ttgtaaaagg cagtaatggg gtgaaacaac cgaatgcatc ctgtacggta accgaatgat
    11461 tcccagaagt aagatttgtg aaattatatg ggccgggtgc cgtgaaagga gttcccccat
    11521 caagtacata ggtaaacggt ccgttcgttg tagagggggt caccagtgct gatccgttac
    11581 cggctccgtt acaggaagtg gcagcagtgg caatggccgc ctgcatctga ttcaggttga
    11641 ctgtaaccgt atccatggaa accagctgac cgccagaccc gtcacataca gaataggtgg
    11701 ttctgacgac aaactgtgta gaacctgtat ttacacaaac gttggggaaa ctgagatcaa
    11761 gtaagccggc aacagtagtg gaggaatccg cggtggaaac cagtgtgcca cccatggtaa
    11821 acatttccga gcgtacaaaa cgggaagccc cgcctgaagg aatgaatttg tacccggaat
    11881 tattctctgt ccagacagca ttattcttac ccac
//
LOCUS       DAOPMW010000065        11831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5714_length_11831_cov_5.874575, whole genome shotgun sequence.
ACCESSION   DAOPMW010000065 DAOPMW010000000
VERSION     DAOPMW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5714_length_11831_cov_5.874575"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLU11_05220"
     CDS             <1..917
                     /locus_tag="PLU11_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58546.1"
                     /translation="FIGGYFLIYFMLERFIYRKIKLIYKFIYKTKATKREETYYKYVL
                     PKKSIDDVREDVEAWAAKNEEELELLRSNEQFRKEFLQNLSHEFKTPVFAIQGYVDTL
                     LSGAMTNPEVNKRFLEKTSKNVDRLSHLLTDLDEISKLERGELVLYKQNFVIQDLIKD
                     VYESLSGKAEKQHIICTIKKGCESPLTVFADKEKIRQVLINLVENSIKYGKQNGNITA
                     SMYKTDDKHILVEIGDDGIGISEKNVVRVFERFYRTEEGRSIDVTGSGLGLAICKHII
                     EAHGQTIHVRSTEDVGTSIGFTLDKRKD"
     gene            complement(948..1550)
                     /locus_tag="PLU11_05225"
     CDS             complement(948..1550)
                     /locus_tag="PLU11_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPN58547.1"
                     /translation="MSFLQIITYSGIFIFAISGALKARTFKMDVFGGIVIAFVTAYGG
                     GTLRDLLIGVKPVNWINDNFALLLVFSGTAITFLLKENAKRFRRTIFYTDAFGLGLFT
                     AWGIEVAMRNGLNEGYALVMGVITATFGGLIADILCNSVPNLLKKGELYATACAIGGA
                     VYLLLKQVPLQYHSNLTVCVLVVAGIRIYASRKKLMLPEI"
     gene            complement(1937..2341)
                     /locus_tag="PLU11_05230"
     CDS             complement(1937..2341)
                     /locus_tag="PLU11_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58548.1"
                     /translation="MRAFILLALIGIYFSSCSSKSPIAKQLAGCDSLVITFNVPGTDS
                     VSNVVSTNDTRAIAKLAGYLGGKEQPKPDCGFGGNMLFFKGGQQVLPVVFQLTKDCSY
                     FMYETESKLHYTKVGSEAGKFLKSLNEGKNWY"
     gene            complement(2458..3897)
                     /locus_tag="PLU11_05235"
     CDS             complement(2458..3897)
                     /locus_tag="PLU11_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPN58549.1"
                     /translation="MKEELVRLANELERIDEFMLAAERDFDYLLEKVHPSNRKSAVNL
                     LHYLALRSLDIRNLQDALHEYGLSSMASSESHIRGQLLAILNRIGNDTVLPEQVFDYQ
                     KSKEFFSERATALFGKRQTESVPWIMVTFDTEFADDYSKVKNLIQSGMNVARINCAHD
                     DESVWFRMIQQVKRATKITGLSCRIYMDLAGPKIRTITRNNEKIAIKEGQHLYLADEQ
                     GIGDDEHTIGCTIPGIAEQLNTGETVLFDDGLIEARVEKIEPGRARLQVLRVSTKKPF
                     LKPEKGINFPESLLSMPALTEYDIKCLSFINKHADMVGYSFVRNTKDLLQLQEAMGRN
                     KKVALIIKIETPEAVKNLPDLLFAGMREEQVGVMIARGDLAVEIGFERMSEIQEEILW
                     ICEAGHVPVIWATQVLENMNKSGMATRSEITDAAHAALADCVMINKGTHIIRTMETLR
                     DILLRSGGHHAKKRYTFRPLAIASRFMTK"
     gene            complement(3979..5073)
                     /locus_tag="PLU11_05240"
     CDS             complement(3979..5073)
                     /locus_tag="PLU11_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN58550.1"
                     /translation="MKSIWTCFVLGIVLTPVIAKHEFTSVAKTPLPVTRNLFIITVDG
                     FRWQELFNGADSSILHSEKFTPDHSTIGLQYWASSAKERREKLMPFFWKVIATKGQVF
                     GNRHWQNKVDAANLYSLSYPGYNEMFTGNTDMQISSNRKKRNPNINVFEYLNRQPDFA
                     GKVAVFSSWDVFPFILNSERNGLPVNSGYVATNGETDAQQMINTVQRETITDKKATRY
                     DQLTFLAAREYLQINQPRVLCLGLGETDEYAHDGRYDLYLQQASQVDRMIADLWHWVQ
                     TTPGYKNNTTFIITTDHGRGSRTGNWTSHGEFISGSSQAWFAVMGPGIEPAGEIKSDE
                     QHYLQQLAQTMAGMLGQKFKQAEAPAIALH"
     gene            complement(5140..6483)
                     /locus_tag="PLU11_05245"
     CDS             complement(5140..6483)
                     /locus_tag="PLU11_05245"
                     /inference="COORDINATES: protein motif:HMM:NF004755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPN58551.1"
                     /translation="MDESIRHLYQEGDVNFSEGRGEWYSQLNPQTLEWLSRDAAVFLH
                     QSLSTPCLNVIESCEGIYLTDINGKRYMDFHGNSVHQVGYRNSYVTDRLKAQLDILPF
                     SPRRYTNKVAIELAEKLVSLLPASLNRVLFAPGGTSAVGMALKLARRITGKQKVVSVQ
                     GSFHGASLDAIAVGGEEQFRKYMGQLYPETIFIPQADRYRNGPDQELQFAELLDEVLT
                     RNEDAGAFIAETIRNTDVQIPSKPYWEKIREVCDRHQVLLILDEIPIAFGRMGTLFAF
                     EQYGIVPDILCLGKGLGAGAFPIAAIITKEEYNIAGDISLGHYTHEKSPMGAAAALAM
                     IDYTIDQHLIEKVQADESFIRKELTIFKDEFPMIGDIRGKGLLWAVELVKDPLTKERA
                     VEEAEAVLYYCLKHGLSFKVSQGNVLQLCPPLIITREQITDALLIIRGALQSVQS"
     gene            complement(6494..7615)
                     /locus_tag="PLU11_05250"
     CDS             complement(6494..7615)
                     /locus_tag="PLU11_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03364 family FAD-dependent oxidoreductase"
                     /protein_id="HPN58552.1"
                     /translation="MLKNAIVIGAGIAGLATARALALKGYKVRVIERSGKAVGASIRN
                     FGMVWPVGQPEGEWYETALRSRSIWKEVCLQAGIWFDEVGSLHMAYAPDEWQVMNEFA
                     AAAEKSRGCRMLTLAETNSMSGAVINYKLQGALYSPDEVIIDSRKAIATIPAWLEEKY
                     GVEFTWGRAVTAVRYPKVEIGKETVEADLVVVCSGPDFESLFPDEYASAPLTKCKLQM
                     MRMIAQPDNWRIGPALCGSLSLVHYKGFAATPSLRQLKQRYQQEYPDYLKWGIHVMVS
                     QNEAAELTIGDSHEYGDTHDPFDKQFINQLILDYLKTFARFRDERLIESWNGVYPKLT
                     DGSEYLALQPENGVFIFNGLGGAGMTLSFGLAEKLMNGF"
     gene            complement(7618..8901)
                     /locus_tag="PLU11_05255"
     CDS             complement(7618..8901)
                     /locus_tag="PLU11_05255"
                     /inference="COORDINATES: protein motif:HMM:NF039829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5690 family protein"
                     /protein_id="HPN58553.1"
                     /translation="MNTLSLQLSRKEWQVSMLAALSAFCVYTCMYAFRKPFTAADFSG
                     ISFAGVDYKIWLVIAQTIGYTASKFFGIGFIGSMQTDKRAPAILKLIFLSWISLLFFA
                     LFPAPFNVLFLFFNGFPLGVIYGLVFSYLEGRRTTELLGAVLSASFIFASGFAQSAGK
                     FVLLEWSVSEYWMPWVTGLLFFPVLILFTWLLDKTPLPDQRDIYYRTERKPMSPKRRR
                     DFIKTFFPGLFLLIVSYVMLTIIRDYRSNFASNMWSELGFANNAAVFTQSELPASLLV
                     LVLMSLLILIRKNIRALLINHVVIVAGFLISIFSTILFLEDGISPFWWMTLSGVGLYI
                     GYVPFNCMLFERLIASFRYISNAGFIIYVADSFGYLGSTGILFVKNFTRVDLSWTGFF
                     IRLVLIGSVAGILLVILSALYFKRKHKKSSFHKME"
     gene            complement(8901..9575)
                     /locus_tag="PLU11_05260"
     CDS             complement(8901..9575)
                     /locus_tag="PLU11_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPN58554.1"
                     /translation="MSIKLAVFDIAGTTVMDSNNVAQAFRNAFASHGLEINEADANPL
                     MGYHKPLAIQLMLEKIGVDPDPEFIEEIHADFEKEMVDFYEYDPAVKPMPGAEDIFLL
                     LKEKGIRIALNTGFSKRIADTIVHRFQWKEKGLVDDYIGSNEVAMGRPYTYMISELMK
                     RAGVEDAAEVIKIGDTPVDIEEGRNAGCRLVIAMTTGATTREVLEAGNPSHILNQLSD
                     IPAILS"
     gene            complement(9661..11697)
                     /locus_tag="PLU11_05265"
     CDS             complement(9661..11697)
                     /locus_tag="PLU11_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58555.1"
                     /translation="MWKKGLLLLSGIWLMLSANAQQPVAGTDTSITSMNEVVLTALRS
                     KQENLRVPYTVSAVSGKFLQEFSPRSTPEALMGMPGVFVQKTNHGGGSPFVRGLTGNQ
                     TLLLVDGVRMNNSTFRYGPNQYLNTIDLYTIGRIEVAKGTGSVQYGTDAMGGVVQIFT
                     KEPEFSLSGNKLTGRLTGKYMTGGMEKTGRGEIAFANKKVALLAGISKKDFGDLIGGD
                     TTGKQSPSGYDEWGLDLNLKFSLQKNTTLQFVSQLLQQQHVPVYHKVRLENFQLNEMD
                     PQRRLFSYARLRKVNGSRIFKETELTVSLQEGKEGRNNQKNGSQVLRQERDRVRTAGL
                     TLDINSVLTRHWSANSGIEVYLDKIFSTREDINLSTAVADQKRGLYPDRSVYGNYSAY
                     SLHHFNLGRWLLDAGIRFNQFTAKISDTTIGDVRINPSAFVYNAAILYKLSKTQSVYL
                     SYSSGFRAPNIDDMGTLGIVDFRYEIPAAGLQPEKSAQTELGYKIRGRRVSAAAAFFY
                     MDISNLITRVREEGQVISGYPVYRKENTDAAFLRGTEFSFSAQITGKLDIKGGLAYTF
                     GQSLTRQEPLRRIPPFNGRGLVTYRNNAWFAAAEWQFATAQKRLAQGDKEDNRIPAGG
                     TPGWNLFNLYGGYKLKQVSFSLGLQNLLHEDYRTHGSGINGVCRSGWLSATFVF"
BASE COUNT         3249 a         2950 c         2541 g         3091 t
ORIGIN      
        1 tgtttatcgg cgggtatttc ctgatctatt ttatgctgga acggtttatt taccgaaaga
       61 tcaaactcat ttataaattc atctacaaga ccaaggccac caaacgggag gaaacctatt
      121 acaaatacgt gttgcccaaa aagagtattg atgatgtacg ggaagatgtg gaagcctggg
      181 ctgccaagaa tgaagaagag ctggaactgt tgaggagtaa tgagcagttc cggaaagaat
      241 ttctgcagaa tctttcccac gaatttaaaa cgccggtatt tgctatacag ggttatgtgg
      301 acaccctttt atctggtgcc atgaccaacc cggaggtcaa taaacgtttt ctcgaaaaaa
      361 catccaagaa tgtagaccgt cttagtcacc tgctcaccga tctggatgaa atctccaagc
      421 tggagcgggg cgaactggtc ttatacaaac agaattttgt catccaggat cttattaagg
      481 atgtgtacga gagtttatcc ggcaaagccg aaaagcaaca tatcatctgc accattaaga
      541 aaggatgcga atctccgctc accgtttttg ccgataagga aaaaataagg caagtgctga
      601 tcaacctggt ggagaactca atcaaatacg gcaaacagaa cggcaacatt acggccagca
      661 tgtacaagac cgacgacaaa catatccttg tcgaaatcgg cgatgacggc atcggcatca
      721 gtgaaaaaaa tgtggtccgg gtctttgaac gtttttaccg caccgaagaa ggccgcagca
      781 ttgacgttac cggcagtggc ctcggtctcg caatctgcaa acacattatc gaagcgcatg
      841 gacaaacgat ccatgtaagg agtaccgagg atgtggggac gagtatcggg tttacgttgg
      901 ataagaggaa ggactagtaa gcagccagca gttagcagtc gccaggctca aatttccggc
      961 aacatcaact tcttccggct cgcatagatc cggatcccgg ccaccaccag tacacagaca
     1021 gttaaattgc tatggtattg caagggcact tgtttcagca agaggtagac cgccccgccg
     1081 atggcgcagg ccgtggcata caattcgcct ttcttgagca ggttgggcac tgagttacaa
     1141 aggatatccg cgatcagtcc gccgaaagtg gcagtgatga cgcccatcac cagggcatag
     1201 ccttcattca atccattgcg catagccact tctatacccc aggcggtaaa caatcccagt
     1261 ccgaatgcat cggtataaaa aatggtgcgc cggaaacgtt tggcattttc tttcaataaa
     1321 aaagtaatag ctgttccgct gaacaccagc aacaaggcga aattatcatt gatccagttc
     1381 accggcttca ccccaatcag cagatcacgc aacgtaccgc ctccataggc cgtaacaaag
     1441 gcaatgacga taccgccaaa tacatccatc ttaaaggtac gcgccttcag ggcaccactg
     1501 atggcgaaga tgaatatacc gctgtacgtt atgatttgga gaaaactcac gtgttggaat
     1561 tttatttttt tttatggtta ttgggaataa agaaccaaga agccaagaca ccaagatgcc
     1621 aaaataagat ccaagaacca agataccaag atgccgaaat aagatacaag aaaccaagat
     1681 ccaagatgcc gaaataagat acaagaacca agacgccaag atgccgaaat aagaaccaag
     1741 aaaccaagac ccaagatgcc aaagaaggac caagatacaa ggatcaaaga aggaccaata
     1801 aataaattcc aaaaaagcac caatcctcct gagaactttc acaggaggct ttggtcattc
     1861 cttttggtta ttttttgacg tttgatattt gatttttctt tggtgtcttg gtgtcttggt
     1921 tcttggatct tttcttctag taccagtttt taccttcatt caaactttta agaaatttcc
     1981 cggcttcact tcccactttg gtgtaatgca gtttactctc cgtttcatac ataaagtagc
     2041 tgcagtcctt cgtcaactga aacacaacag gcagtacctg ctgtccgcct ttgaagaaaa
     2101 gcatattgcc gccaaaaccg caatcgggtt tgggctgctc ttttccgcca agataaccgg
     2161 ccagtttggc aatagcccgg gtatcgttgg tgctgaccac attactcacc gaatcggtac
     2221 ccggcacatt gaacgtaatc acgaggctgt cgcagccggc gagttgtttg gcaatggggg
     2281 atttggagga gcaggaggaa aaataaattc caatcagcgc aagtaaaata aatgcccgca
     2341 taattttttt tcaaagatag aaaaagcccc tgtgaaaaca ggggccgtag ttaactatta
     2401 gcattcaaaa accgggtgca ggcaattgtc gacccgttta aaaaaattat atttcgatca
     2461 ttttgtcata aacctactgg caatggccag gggtctgaaa gtataacgtt tcttagcatg
     2521 gtggccgcca ctccgtagca ggatatcccg cagcgtttcc atggtacgga taatatgggt
     2581 tcctttattg atcatcacac aatctgccag tgcggcatgg gcggcatcgg taatttcaga
     2641 tcgggtggcc atgcctgatt tattcatatt ctccaatacc tgtgtggccc agatgacggg
     2701 tacatgtccg gcctcacata tccagagaat ctcctcctga atttcactca tccgttcaaa
     2761 accaatctcc accgcgaggt caccacgggc aatcatcacc cccacctgct cttcccgcat
     2821 accggcaaaa agcaggtcgg gcaggttttt aactgcttcc ggtgtttcaa tttttatgat
     2881 cagggcgact ttcttatttc gtcccatcgc ttcctgcaac tgcagcagat ccttggtgtt
     2941 acggacaaaa gaatacccga ccatatccgc atgcttgttg ataaaagaaa gacatttgat
     3001 atcgtactcc gtcagggccg gcatggacaa caaggactcc gggaaattga ttcctttctc
     3061 gggttttaaa aaaggcttct tggttgaaac ccttaatacc tgcaatctgg cccgccctgg
     3121 ctctatcttc tctaccctgg cttcgatcag cccgtcgtca aacaatacgg tttccccggt
     3181 attcagctgc tcggcaattc ccggaatcgt acacccaatt gtatgttcat catcaccgat
     3241 tccctgctcg tccgccaggt acagatgctg accttccttg atagctattt tttcattatt
     3301 ccgggtaatg gttctgattt tcggaccggc aaggtccata tagatacggc aactgagtcc
     3361 ggtgattttt gtggcgcgtt ttacctgttg tatcatccgg aaccagactg attcatcatc
     3421 gtgggcacaa ttaatccggg ctacattcat cccggattgg atcaggtttt taacttttga
     3481 ataatcatca gcaaactccg tatcaaaagt gaccataatc catggaacgg attctgtttg
     3541 tctttttccg aataaagcag tggccctttc tgaaaagaat tctttgctct tctgatagtc
     3601 aaatacctgc tccggaagca ccgtgtcatt cccgatacgg ttcagaatag cgagcagctg
     3661 accgcggata tgactttcag aactggccat ggaagaaagt ccatattcat gcagtgcatc
     3721 ctgcagattc cttatatcca aacttcgtaa agcgagataa tggagcagat ttactgcgct
     3781 tttccggttg gaaggatgta ctttttcaag caaataatca aaatcccgct ccgccgccaa
     3841 cataaattca tcaatccgtt ccagttcatt ggccaacctg accagttctt ccttcatttg
     3901 aaatatttaa acaaattaat aaagaggtcc gcaaactaac actgatgcag attagagtgc
     3961 tgaatgattt tatacggatt aatgcaaggc gattgcaggt gcttctgctt gtttgaattt
     4021 ctgtcctaac attccggcca tggtttgagc caattgctga agataatgct gctcgtcaga
     4081 cttaatttcc ccggctggtt caatgcccgg acccataaca gcaaaccagg cttgtgaaga
     4141 accactgata aactccccat gtgaagtcca gttaccggta cgactgccac ggccatgatc
     4201 ggtagtgatg atgaaggtgg tattattttt atacccgggc gtggtttgca cccaatgcca
     4261 gagatcggca atcatccggt ccacctggct ggcctgttgc aaataaagat cataacggcc
     4321 gtcgtgtgca tactcatccg tttctcctaa tccaaggcat aaaacacggg gctggttgat
     4381 ttgcaggtat tcccgggcag ccagaaaggt cagctgatcg taacgggtag cttttttatc
     4441 cgtgatggtt tcccgttgaa cggtattgat catttgttgc gcatcggtct ccccattggt
     4501 agcaacgtac ccgctattaa caggtaaccc gtttctttcc gaattgagaa taaaaggaaa
     4561 tacatcccag gaagaaaata cggctacttt accagcgaaa tccggctgcc ggttcaggta
     4621 ctcaaataca ttaatgtttg gatttctttt cttccggttg gaggaaatct gcatatccgt
     4681 attgccggta aacatttcat tataccccgg ataagaaagt gaataaagat tagccgcatc
     4741 cactttgttc tgccaatgcc ggttgccaaa tacctggccc ttcgtcgcga tcactttcca
     4801 gaaaaaaggc attagtttct cccggcgttc tttcgctgaa gatgcccagt attgcagacc
     4861 gatggtggaa tggtcgggtg tgaatttttc cgaatgaaga atggaggaat cggccccatt
     4921 gaataattcc tgccagcgga acccatcgac agtgatgata aagagattac gggttacagg
     4981 caacggcgtt tttgcgaccg atgtaaattc gtgtttggca atcacaggtg tcagcacaat
     5041 cccaaggaca aaacaagtcc agatgctttt catgtgtaac ggttttgcaa agaaagcaag
     5101 ccgatataac ctcaatatta agtatagatt acggaattgt taggattgca ccgattgcaa
     5161 agctccgcgg attattaata aggcatccgt tatctgttcc cgggttataa ttaagggggg
     5221 acaaagctgt aatacatttc cctgggatac tttaaaactc aacccgtgtt ttaaacaata
     5281 atacaaaaca gcttctgcct cctcgacggc tctttctttg gtcagaggat ccttaaccag
     5341 ttccactgcc cataataatc cctttcccct gatatctccg atcatcggaa attcatcttt
     5401 gaatattgtc agctctttcc ggataaaaga ctcatccgct tgcacctttt ctattaaatg
     5461 ctgatcgatt gtataatcga tcatggcgag ggctgcggct gcacccatcg ggcttttctc
     5521 gtgcgtataa tgtcccagtg aaatatcccc cgctatattg tattcttctt tggtaatgat
     5581 cgctgcaatc ggaaatgcgc cggcacccaa tccttttccc aggcaaagta tatccggtac
     5641 aatgccatat tgttcaaagg cgaacagggt tcccattctt ccgaatgcaa tgggaatctc
     5701 atccagtatc agcagtacct ggtgacggtc gcatacttcc ctgatttttt cccaatacgg
     5761 cttcgaagga atctgcacat cggtattccg gatggtttct gcgataaaag cccctgcatc
     5821 ctcattacgg gttaacactt catccagcaa ctcagcaaac tgcagttcct gatccggtcc
     5881 attccggtat ctatcggcct gtggaataaa tatagtctcc gggtaaagtt gtcccatgta
     5941 tttcctgaac tgttcttccc cgccaaccgc aatggcatcc agcgaagccc cgtggaaaga
     6001 accttgcacg gaaaccactt tttgttttcc ggtaatccgg cgggcaagtt tcagggccat
     6061 acctactgcg ctggttccgc ccggggcaaa caatacccgg ttgagggaag ctggcaataa
     6121 actgaccagt ttttcggcca gctctatagc gactttattg gtatagcgcc tcggcgaaaa
     6181 gggtagaata tccagctgcg ctttcaatcg gtcagtcaca tacgaatttc tatatccaac
     6241 ctggtgaaca ctattgccat gaaaatccat gtaacgcttt ccgttgatat cggtcaggta
     6301 aatgccttcg caggattcaa tcacattgag gcaaggggtg gacagggact gatgcagaaa
     6361 aaccgctgca tccctggaca gccattccag tgtttgcggg ttgagctggc tgtaccattc
     6421 cccgcggcct tcggaaaaat tcacatcccc ctcctgatat aaatgacgta tggattcatc
     6481 catagctatt tgtttaaaag ccgttcatta atttttcggc caaaccaaat gacaaagtca
     6541 tacctgcccc acccaacccg ttaaaaataa atacaccatt ttccggttgc agggctaaat
     6601 actcagaccc atcagtgagt ttgggataga ccccattcca ggattcgatg agtcgttcat
     6661 ccctgaaacg ggcaaaggtt ttcaggtaat ccaggatcaa ttgattgata aactgtttat
     6721 caaagggatc atgggtatct ccgtattcat gcgaatcgcc gatggtgagt tcagctgcct
     6781 cattctgaga caccatgaca tggattcccc atttaagata gtccgggtac tcctgctggt
     6841 agcgttgttt taattgcctg agtgagggag ttgcggcaaa tcctttatag tgtaccaggg
     6901 aaagtgagcc acataacgcg gggccaattc tccagttatc cggctgggca atcatccgca
     6961 tcatttgtag tttacatttg gtcagcggag cggatgcata ttcatccgga aataggcttt
     7021 caaaatcagg tccgctgcaa accaccacca ggtctgcctc cactgtttct ttaccgattt
     7081 ccaccttcgg ataccgcaca gccgtaacgg ctcgtcccca ggtaaattcc acgccatatt
     7141 tctcttccag ccaggcgggg atcgtagcaa tggcttttcg ggaatcaatg atcacttcat
     7201 cgggactata aagtgcacct tgcaatttat aattgatcac cgctccggac atactgtttg
     7261 tttcagccag tgtcagcatc cggcagcctc ttgatttttc ggcagcagcg gcaaactcat
     7321 tcattacctg ccactcatcc ggggcataag ccatgtgcag cgatcccact tcatcaaacc
     7381 agatcccagc ctgcagacat acttctttcc agatactccg ggagcggagc gctgtttcat
     7441 accattcccc ttccggttgt cctaccggcc ataccatacc aaaattccgg atggaagctc
     7501 ccacagcttt gccactccgc tctatgaccc ttaccttata cccctttaaa gccagtgccc
     7561 gcgctgtagc caaaccagcg atacccgcac caataacaat tgcattcttt aacatagcta
     7621 ttccatttta tgaaatgaac tcttcttgtg ttttctttta aaataaaggg ccgacagtat
     7681 gacaagcaat ataccggcaa cagacccgat cagtacaaga cggatgaaaa agccggtcca
     7741 cgacaaatcc acccgggtga aatttttgac aaaaagaata ccggtactac cgaggtaccc
     7801 gaatgaatca gccacataga taataaaacc ggcattgctg atatagcgga agctggcgat
     7861 gagtcgttcg aacaacatgc agttaaacgg cacatagccg atataaagtc ctaccccgct
     7921 aagcgtcatc caccaaaatg gtgaaattcc gtcttccaga aagaggatcg tgctgaatat
     7981 ggagattaaa aatccggcga caattactac atgattgatc agcagggccc gtatattttt
     8041 ccggatcagg atcagcaaac tcattaatac cagtaccagc agtgaagcag gcagctcact
     8101 ttgtgtaaac acagccgcat tatttgcaaa acccagttca ctccacatat tagaggcgaa
     8161 attgctccgg taatccctga taatagtcag cattacatag gaaacaatca gcaagaataa
     8221 gcctggaaaa aaagttttga taaaatccct tcgtcttttc ggactcatgg gctttcgttc
     8281 cgtacggtaa taaatatccc gctgatccgg taagggggtc ttatccaata accaggtaaa
     8341 taatataagt accggaaaga acaataaccc ggttacccag ggcatccagt attcagatac
     8401 cgaccattcg agcaacacaa atttcccggc agattgtgca aaaccggaag caaaaataaa
     8461 actggctgat aataccgcac cgagtagctc ggtggttcgc ctgccttcca ggtaactaaa
     8521 caccagaccg tagatcacgc cgagcggaaa cccattaaaa aaaaggaaca acacattaaa
     8581 aggtgccgga aacaaggcaa aaaacaaaag tgaaatccag gataaaaaaa taagcttcag
     8641 aatggccgga gcccgtttat cggtctgcat agaaccaata aaaccgatcc cgaaaaactt
     8701 gctggcagta tacccgatcg tctgtgcaat gacaagccat attttataat caacgccggc
     8761 aaagctgatt cctgagaaat cggctgcggt aaaaggttta cggaatgcat acatacaggt
     8821 atacacacaa aaagctgaaa gggcagcgag cattgatacc tgccattctt tcctgctcag
     8881 ttgcaaggag agagtattca tcaggagagt atagcaggta tatcagaaag ctggttcaga
     8941 atatgactgg gattgcctgc ttcgaggact tcccgggtgg tagccccggt agtcatggca
     9001 atgactaagc ggcaacctgc attccgtcct tcttctatat ctacgggggt atccccgatt
     9061 tttattactt cggcggcatc ttccacgccc gcccgcttca tcagctcact gatcatataa
     9121 gtataagggc gacccatagc tacttcattg ctcccgatat aatcatccac caaccctttt
     9181 tctttccatt gaaaacgatg cacgatggta tccgcaatcc gctttgaaaa accggtattc
     9241 aatgcaatac ggattccttt ttcctttaac agtaaaaaaa tatcctccgc gccgggcatt
     9301 ggttttactg ccgggtcata ttcataaaaa tcgaccatct ccttttcaaa atccgcatga
     9361 atctcttcta taaattccgg atccggatcc accccgattt tttccagcat cagctgaatg
     9421 gccaagggct tatgatagcc catcagggga ttggcatccg cttcgttaat ttcaagaccg
     9481 tgactggcaa aggcattcct gaacgcttgg gctacattat tactatccat gacggtggtg
     9541 cctgctatat caaatacagc gagtttgatt gacatactaa atatttcccg aaaggtattg
     9601 ggaagtaata gtatgcctgt taagccagca tgaacaaacc tttaactctg tgtgattaca
     9661 tcagaagacg aaagtggcag aaagccatcc ggagcgacac accccattga taccggaacc
     9721 atgggtgcgg taatcttcat gcaacaggtt ctgcagtccc aatgaaaagg agacctgctt
     9781 tagtttatat cctccgtaaa gattaaacag gttccagcct ggtgtacccc ctgcaggaat
     9841 acgattatct tccttatcgc cctgtgccag tcgtttctgt gcagtagcaa actgccattc
     9901 agccgcggca aaccaggcat tattcctgta cgtgacgagc cctcttccat taaatggggg
     9961 tatccggcgc aagggttcct gccgggtcag actctgtcca aaagtatagg ccagtcctcc
    10021 tttgatgtca agcttaccgg taatctgtgc cgaaaatgag aactccgtac cacgtaggaa
    10081 ggccgcatct gtattctctt tccggtaaac gggataccca cttatcactt gtccttcttc
    10141 cctgacccgg gtgatcagat tgctgatatc catataaaaa aaggcggccg ccgcactgac
    10201 tctccttccg cggatcttat aacccagttc tgtctgagca gatttctccg gttgtaatcc
    10261 ggcagccgga atctcatacc tgaaatccac aataccgagg gtacccatat catcaatatt
    10321 cggcgctcgg aaaccgctgc tgtaggaaag atatacagac tgcgttttgc tgagtttata
    10381 taaaatggcg gcattgtata cgaaggctga gggattgata cgtacatcac caatcgtggt
    10441 atccgaaatc tttgcagtaa actgattgaa acgaatccct gcatccagca accagcggcc
    10501 aagattgaaa tgatgaagtg aataggccga ataattgcca tagaccgaac gatccgggta
    10561 tagtcccctt ttctgatcag ccacagccgt ggagagattt atgtcttccc tggtgctgaa
    10621 aattttatcc aggtatactt cgatacctga atttgcggac caatgcctgg taagcaccga
    10681 gttaatatca agggtcagac ccgcagtacg aaccctgtcc cgctcctgtc gtaaaacctg
    10741 gctgccattc ttttggttat ttctaccttc tttcccttcc tgcaatgaaa ccgttagttc
    10801 agtttccttg aaaatccgtg aaccattgac tttccggaga cgggcataac tgaataaccg
    10861 ccgctgggga tccatttcat ttaactgaaa attttccagc cgcactttat gatagaccgg
    10921 cacatgctgc tgttgtaata actggctgac aaactgaagg gttgtattct tctgcaggct
    10981 gaacttcaga ttcaggtcca atccccattc atcatatccg gaaggagatt gtttgcccgt
    11041 ggtatctccg ccgatcagat cgccaaaatc cttcttacta ataccggcta gcaaggctac
    11101 cttcttgtta gcaaatgcaa tttctccccg gccggttttt tccatgcctc cggtcatata
    11161 tttcccggtc agacggccgg taagtttgtt tcctgacaat gaaaattcag gttccttcgt
    11221 aaaaatctgt accacccctc ccatcgcatc ggtaccatac tgtacagaac cggttccttt
    11281 ggccacttcg atccggccga tggtatacag gtcaatggta ttcaggtatt ggttgggacc
    11341 ataacggaaa gtagagttat tcatccgtac cccatctacc agtaacaaag tctgattacc
    11401 ggtcagtccc cttacaaaag gagaaccgcc cccatgattt gttttctgca caaatacacc
    11461 cggcattccc atcagggctt ccggagtact ccgcggactg aattcctgca ggaatttacc
    11521 ggaaactgca ctaacggtat aaggcacccg cagattttcc tgcttactcc gcagggcggt
    11581 gagtaccact tcattcatgg aagtgatgct ggtatcagtt ccggcaaccg gttgttgcgc
    11641 attggccgac agcatcagcc aaatacctga cagtaacaat agtccttttt tccacatgat
    11701 ttaaaacatt aatcttcttt ctccgttttt ttcagaaatg cactccggcg gggtgctggc
    11761 attttacttc cttctccttt caccgctttc cagattactt cattgaaaac cagatccggt
    11821 atggcatctt c
//
LOCUS       DAOPMW010000066        11546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5923_length_11546_cov_9.030807, whole genome shotgun sequence.
ACCESSION   DAOPMW010000066 DAOPMW010000000
VERSION     DAOPMW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_11546_cov_9.030807"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1952)
                     /gene="aspS"
                     /locus_tag="PLU11_05270"
     CDS             complement(126..1952)
                     /gene="aspS"
                     /locus_tag="PLU11_05270"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPN58556.1"
                     /translation="MYRTHTCGELRATHTGNQVTLAGWVQTVRKFGAITFVDLRDRYG
                     ITQLLFGEDLNKELEDSPLGREFVLQATGTVSERSNKNLNIPTGDIEIAISSYKILNK
                     SAVPPFTIQDDTDGGDDLRLKYRYLDLRRNAVKRNLELRYAVNRAARNYLHNQGFMDI
                     ETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGYDRYYQIVKCFRD
                     EDLRADRQPEFTQIDCEMAFVEQEDILSMFEGLIKSVFKDVKGIDYTDAVERMSWEDA
                     MWNFGNDKPDIRFGMKLLNLKKPGSVYTNKQGESALIDGADFKVFDEAETVIAIAVPG
                     CSEYSRKQTDELTEWVKRPQIGMKGLVFIKCNTDGTYKSSVDKFYSEEKLKAIADAAG
                     AKAGDLVLILAGAEERTRKAVSELRLEMGKRLGLRKDDEFRLLWVLDFPLFEYDEEGN
                     RWVARHHPFTSPKPAQIETMINNNPVIENAAEYLQHPYANIKANAYDMVLNGNEIGGG
                     SIRIFQRELQEKMFAALGMDKEEQLHKFGFLLGAFEYGAPPHGGIAFGFDRLCSILGG
                     SESIRDFIAFPKNNSGRDVMLDAPATIDEKQFEELQIKLNLK"
     gene            2122..3138
                     /locus_tag="PLU11_05275"
     CDS             2122..3138
                     /locus_tag="PLU11_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPN58557.1"
                     /translation="MPGDTTITPVTESAGFTDYYISQKGDTIWVQPPPPGEAYQQSQR
                     HNNAAGESKSMDTADLAIIFGIFILLFALAGRKKKEEELPYAERSPESRRKQAEEKAM
                     EHGAVYDEWLSSYNPYYKGLSKDLRERFLLRTIEFAQAKEFRFHEIEEEDYMLILISG
                     AAVQMTFGLRNFLMDYFPVIHIIRKEYTLHHDNETYYGHVSRNGIYISWSHFLAGYKD
                     YSDSVNVGLHEMAHAVSYDVFLGLQDRHDRKFKQRLQEFRLEGGPIFRALKEGDPHLL
                     DDYATTNFDEFWAVCVETFFENPEEFRNEEPGLYQSICDLLNQDPTRPGKIIDKRIAG
                     IRET"
     gene            complement(3139..3549)
                     /locus_tag="PLU11_05280"
     CDS             complement(3139..3549)
                     /locus_tag="PLU11_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58558.1"
                     /translation="MNQNNEAVYSIPAQYRRMENMHIVFWLVKDISWCMIWRELGLAM
                     FIPTLTIAIVIAWRTRQLKSELAHNLAIAFWITANGYWMISEFFHFDEYIVYGTWTGK
                     HMALIPFLIGAAILLYYYAILRPREIRENKTTTM"
     gene            complement(3573..4640)
                     /locus_tag="PLU11_05285"
     CDS             complement(3573..4640)
                     /locus_tag="PLU11_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN58559.1"
                     /translation="MKVDYLLIGQGISGTWLSYFLQKENKSFLVIDDKNPNAPSRLAA
                     GIINPVTGRRHVSTWMIDTLLPYVWEQYTTLGNELGITAISQRNVLDFFPSPQMRESF
                     IKRVEEKGEYVSVPDDPNMFRPLFNYEFGCGEIKPVYTAHLETIIPAWRNKLATEGLL
                     LEEKLKTDDLVVAKDHIRYKEIEAKKIIFCDGAACAANSWFELLPFAPNKGEALFVSI
                     PGLPKDRIYKKGMLLVPMVQPDQWWLGSAYEWEFKDELPSAEFRKKGEQLLQQWLKLP
                     YTIQDHIASIRPATLERRPFVGLHPVHHSIGILNGMGTKGCSLAPFFAAQLVRHLVYS
                     EPIDPEGDVKRFTRILSRNIS"
     gene            complement(4746..5276)
                     /locus_tag="PLU11_05290"
     CDS             complement(4746..5276)
                     /locus_tag="PLU11_05290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPN58560.1"
                     /translation="MKLIIARHGVTLENATNIAQGQTDGTLSAEGKAANEELGRELQQ
                     HFFKTIYSSPLGRALQTAEAIQRYNPHTHLMVDHRLMERHLGILQEKPYPVPYAEADV
                     YEGMETADSMKARLNEFLAEIIQQHKEETIVLVSHGYLIKVLLSILHGWPVEDFHQVK
                     LMGNSAYTIEVLRYDS"
     gene            complement(5377..>7080)
                     /locus_tag="PLU11_05295"
     CDS             complement(5377..>7080)
                     /locus_tag="PLU11_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HPN58561.1"
                     /translation="VQALPYLMEYPYDCAEQTWNRYYANSLASYITSASPKIKQVFEQ
                     WKIKDTAALMSNLEKNQELKAVLLEETPWVLEAKTEAQQKKNIALLFDMVKMSEALNS
                     SFAKLKKMQGPNGGFVWFSGGPDDRYITQYIVTGIGHLKKLNGIAPGQEGYLKSILTL
                     ALPYLDKKIKADYDGLIKNKVNLKTYSPGYSEIQYLYMRSFFPDYEITKGTKTAYDFF
                     RGKLAVNWVKQSKYMQGMIALALYRTGDIKTPVAILKSLKETSITHEELGRYWKDQRY
                     GWFWYEMPIETHALLIEAFTEAGKDQATVDELKTWLLKNKQTNNWKTTKATAEACYAL
                     LMQGSNWLTNEPVVEIKLGNTTFKSTDAGAEAGTGYFKKTIEAQKVNPEQGNISISVN
                     QPLNISTTKQLNSSSSWGSVYWQYFEDLDKITTAQTPLKLSKQLFIQTNSDRGPVLTP
                     VTEGEAIKVGDKIKVRVELRVDRDMEYVHMKDMRASALEPVNVLSSYKWQGGLGYYES
                     TKDASTNFFFSFLRKGTYVFEYELFATHAGEFSNGITSIQCMYAPEFTAHSEGIRITV
                     E"
     assembly_gap    7081..7180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7181..11295)
                     /locus_tag="PLU11_05300"
     CDS             complement(<7181..11295)
                     /locus_tag="PLU11_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPN58562.1"
                     /translation="MKMRLQHNLILTILFTCFFVMNSQSQTPVKNYDKEWKKVEQFSQ
                     KGLPQSALTEVKKIYALAKKEKQDAQVIKSLLYISGLQSDTREDNDVLSIREMETELK
                     TSKEPVTALLKNIIAGMYWNYYQGNRWELMDRTQTAKFNKTDIATWGAEDFHKKISEL
                     YLQSLKDTRLLQQTKLDPFNAIIIKGNVRHLRPTLYDLLAHHALNYFENDERDITKPA
                     YAFEIDQSSAFDPAADFIKRKFTTKDSLSLQHKALLIYQELIAFHLNDTKPDALIDAD
                     LKRLGFVKENSTHPDKDQLYFNSINHIARQYENHPAAAQAWYLLALYYNEQANDYKPH
                     GDTTFQYARIKAKEIAEKLQLQKDSSEGKINAGNLLVELNRSSFEFQLEKVNSPDMPF
                     RSLVTYKNVTTLHLRLIRSDEKLRTQLENQYNEKYWPTLVSAPVMKSWQQPLPATNDL
                     QEHKVEIKIDGLPAGEYILLASDNADFKNAKSLLGARSFYVSGISYVNTDQDYFVLHR
                     ETGQPLANASIQVWEQKYDQKQSKNIKLRGKAYTTDANGFFKMERPAKEENNYWNYSF
                     LLDIKHDNDRLFMNEYQNDYYYYRNNEDQNTHGKTAIHLFTDRAIYRPGQTLYLKGIV
                     LALNMDIKKADIRAGYTTTVYLRDANDKDVDSLKLKTNEFGSFNGKFQLPQGSLNGDF
                     SIYTKGENAQTGFKVEEYKRPKFQVEYEPIKGTYKVNDKITITGTAKAYAGNNIDGAM
                     VKYRVVREARFLYPWLLRRWWQPPAQSMEITHGEIKTDKDGKFNVEFTAIPDLKIDKK
                     FEPVFDYTVYADVTDINGETRSGEQAVSVSYKAMKLVTTIPDVLPADSLKTLSIRTVN
                     MNGEFIPATAKVIIHKLKEEKRLIRKRYWERPDQFLMSKEEYVKLFPVDEYDNESEYT
                     NWETGESVYTKSDSVPENGKWRVESGNLTPGFYVIEVTATGKNGEEVKSVEYTELIDE
                     KSNQLNRAAYLWTNKKMGPVEPGETAKFNIGTSAENLFVIRQQINKASNYSYIRLSNE
                     KRTFDTPVNEEDRGGISSLWAFVKNNRFFQYTDITNVPWSNKVLDIEYATFRDKTLPG
                     SEEKWKLRISGNKKEKVAAEILAAMYDASLDQFVEHYWNSPMPWPLNKLQNPWNNSTN
                     FEDLTSSHRSADQQAGKYLEKNYDMLIDNTMAIDNFKYQFDKKTTSRFTYMLGGNAME
                     ASMTPAHPSLKRGEENGMDAAIAEKETSFTTNVPYLKVEEVKNNSNTIEPITIRKNFS
                     ETAFFLPELQTDSTGAIEFSFTMPEALTKWKFMALAHTKDAAFGYSTKEIVTQKELMV
                     QPNAPRFLREGDKMEFSTKIVNLTDKELTGTAQLELFDASTNTSIDGWFRNS"
BASE COUNT         2662 a         2570 c         2748 g         3466 t
ORIGIN      
        1 tataaggtct tcttttggag gattcctgag tgattgaaaa agtagagtag cggaaagtaa
       61 actggaaaaa agtagttgaa ggatggtcgt catccgaaac aggttccagc gtacttcctt
      121 tttcattatt tcaggttcaa ctttatctgc aactcctcaa actgcttctc atcgatcgta
      181 gccggtgcat ccagcatcac atcgcgtcct gaattgtttt tcgggaaggc aatgaagtcg
      241 cggatgcttt cgctgccgcc gaggatggaa cagagccggt cgaaaccgaa ggcaatacca
      301 ccatgcggag gtgctccgta ttcgaaggcg cctagcagga aaccgaattt gtgcagttgt
      361 tcttccttgt ccatgccgag ggccgcaaac attttttcct gcagttcgcg ctggaagatc
      421 cggatggaac caccaccgat ttcattcccg ttcagtacca tatcataggc gttggccttg
      481 atattggcat agggatgttg caagtattcc gccgcatttt caatcacggg gttattattg
      541 atcattgttt cgatctgcgc cggcttcggg gaagtgaacg gatggtgacg ggccacccaa
      601 cggttgcctt cctcatcgta ttcaaataac gggaagtcga gaacccaaag taaacggaat
      661 tcatcatctt tgcgcaagcc caggcgtttg cccatttcaa ggcggagttc gctgacggct
      721 ttacgggttc tttcttcagc accggccagg atcaatacga ggtcaccggc tttggcacct
      781 gcggcatcgg caatggcttt gagtttctct tcactataga atttatcgac actgctttta
      841 tacgtgccgt cggtattaca cttgataaag accagtcctt tcataccgat ctgcggacgt
      901 ttcacccatt cggtgagttc atccgtttgt ttgcggctgt attcgctgca accgggcacg
      961 gcaatggcga tcacggtctc tgcttcatcg aatactttaa agtcggcccc atcaatcagg
     1021 gcagattcgc cttgcttgtt cgtatataca gaacctggtt ttttcaggtt cagcagtttc
     1081 ataccaaagc ggatatcggg tttgtcgttt ccgaaattcc acatcgcatc ctcccagctc
     1141 atacgttcta cggcgtcggt atagtcaatg ccttttacgt ccttaaaaac cgatttgatt
     1201 aatccttcaa acatgctgag gatatcttcc tgctccacga aagccatttc acagtcgatc
     1261 tgggtaaact ccggctgacg gtcggcccgc aggtcttcat ccctgaaaca ttttacgatc
     1321 tggtaatagc gatcatagcc actcaccatc agcagttgct tgaaggtctg gggtgactgg
     1381 ggcagggcgt aaaactgacc aggattcata cgggaaggca cgacaaagtc gcgggctcct
     1441 tcgggggtgg acttgatcag gaacggcgtt tcaatatcca tgaatccctg gttgtgcagg
     1501 taattccggg cggcacggtt cacggcataa cgcaattcca gattccgctt caccgcattg
     1561 cggcgcaggt ccaggtaacg gtatttcagc cgcaggtcat ccccgccatc cgtatcatcc
     1621 tggatggtga agggaggaac ggcagattta ttcaggatct tataagaact gatggcgatc
     1681 tcaatatcgc cggttgggat attcaggttt ttattgctcc gctcgcttac ggtaccggtg
     1741 gcctgtaata caaactcacg gcccaggggg ctgtcctcca gctccttgtt cagatcttcc
     1801 ccgaaaagta attgggtgat gccataacgg tcacgcaggt ccacaaacgt gatggccccg
     1861 aatttacgga cggtttgtac ccagccagcg agggttacct ggttgccggt atgggttgct
     1921 ctgagttcgc cgcaggtatg agttctgtac atagtgagtt gataggttga aaagttgaca
     1981 ggttgacagg gcctttctat tggaggatcg gtactgtccc gggtgttttg tccggggggc
     2041 aaagatagca aatcggggtc tattttccgg gcgtggtcaa gcccttgtct acgaatagaa
     2101 atcctgtaaa tttgatgctt tatgcccggg gacaccacca ttacacctgt tactgaatca
     2161 gccggtttta cagactatta tatttcccag aagggtgata ccatctgggt gcaacccccg
     2221 ccccctggtg aggcgtatca gcaatctcag cgacataata atgctgctgg cgagagtaaa
     2281 tcaatggaca cagctgatct tgcaatcatt ttcggaatct ttatactgct ttttgcactt
     2341 gccggaagaa aaaagaagga ggaggaactg ccgtatgctg aacgatcgcc tgaaagcaga
     2401 agaaaacagg cggaagaaaa agccatggag cacggtgccg tgtatgatga atggctgagc
     2461 agttacaatc catattacaa aggactatca aaggatttgc gtgaacggtt cctgttgcgc
     2521 actatcgaat ttgcccaggc caaagaattc cggtttcatg aaattgaaga agaggattat
     2581 atgttaatcc tgattagcgg agcggcggta caaatgactt ttggattgcg aaattttctg
     2641 atggattatt ttccggtgat ccacatcata cggaaagaat acaccttgca tcatgacaat
     2701 gaaacttatt acgggcatgt aagccggaat ggtatttata tttcatggag tcactttctg
     2761 gcgggatata aagattattc agattctgtg aatgttggat tacatgaaat ggcgcatgcc
     2821 gtttcctatg atgtattttt aggccttcag gaccgtcatg accggaagtt taaacagcgt
     2881 ttacaggagt tccggttgga gggaggtccg atcttcaggg ccctgaagga aggggatccg
     2941 catttgctgg atgactatgc gactacgaat tttgatgagt tctgggctgt ttgcgtggag
     3001 actttttttg aaaacccgga agaattcagg aatgaagagc ccggactcta tcaatccatt
     3061 tgtgacctgc tgaaccagga ccccacccgt ccgggtaaaa tcattgataa aagaattgca
     3121 gggatccggg aaacctgatt acatcgttgt tgttttattt tccctgatct ctcgtgggcg
     3181 cagtatggca taatagtaca acaggatggc agccccgatc aggaagggaa tcagggccat
     3241 atgtttgccg gtccatgttc cataaacaat gtattcatca aagtgaaaaa attcactgat
     3301 catccaatac ccattggcgg taatccagaa ggcaatggcc aggttatggg ccagctccga
     3361 ttttaactgt cgggtgcgcc aggcaatcac aatggcaatg gtgagtgtgg gaataaacat
     3421 ggccagtccc agttcgcgcc agatcatgca ccagctgatg tcctttacca gccagaagac
     3481 aatatgcata ttctccatcc ggcggtattg ggccgggatg ctgtacacag cttcattgtt
     3541 ttggttcatc cgcaaaaatt atagcaagga agttaagaaa tgttgcggga aagtatacgg
     3601 gtaaaccgct ttacatctcc ttcggggtcg atgggttcac tatatacgag atgacggacc
     3661 agttgtgctg caaaaaaagg agccagggaa cagcccttgg tgcccatgcc attcaggatg
     3721 ccgatggaat gatgaaccgg gtgcagtccc acaaaggggc gccgctccag ggtggccgga
     3781 cggatactgg cgatatgatc ctggatggta tacgggagtt tcagccattg ttgcaatagt
     3841 tgttctcctt ttttccggaa ctccgcagaa ggtaattcat ctttaaattc ccattcatag
     3901 gcagagccca gccaccattg atccggctgt accatgggca ccagcaacat gccttttttg
     3961 taaatacgat ccttgggcaa accaggaata ctgacaaata aggcttctcc tttattcggg
     4021 gcgaagggaa ggagttcaaa ccaggagttc gctgcacagg ctgccccgtc gcagaagatg
     4081 atttttttgg cttcaatctc tttataacgg atatggtctt tcgcaacgac caggtcgtca
     4141 gttttcagtt tttcttcaag aagtaatccc tctgttgcga gtttgtttcg ccaggcgggg
     4201 atgatggttt cgagatgggc ggtatatacg ggtttgattt ccccgcaacc aaattcataa
     4261 ttgaagagcg ggcggaacat gttggggtca tccggtacgg atacatattc ccctttttct
     4321 tccacgcgtt tgataaagct ttcccgcatc tggggactgg gaaaaaagtc aagtacgttt
     4381 ctctgcgaaa tggctgtaat acccagttcg ttgccaagtg tagtatactg ttcccaaaca
     4441 tagggaagca aggtgtcgat catccaggta ctcacgtgcc ggcggccggt aaccggatta
     4501 ataatgccgg cagctaaccg ggaaggggca ttcgggtttt tatcatctat caccagaaag
     4561 gacttatttt ccttttgcag gaaataagat aaccaggtgc cggagatacc ctggccaatg
     4621 aggagataat caactttcat ggggcaaatg tagcgcaata tgtcagttta cattcttaga
     4681 attccaatgc gcttgtcatg ctgagtagac agcatcaggt atattagaat gttataacag
     4741 cgacttcagc tgtcgtatcg aagcacttcg atcgtatagg ccgaatttcc catcagtttc
     4801 acctggtgaa aatcctctac cggccagcca tgcaggatcg acaacaacac tttgatcagg
     4861 tatccatgcg aaaccagtac aatggtctcc tctttgtgtt gctgtattat ttccgctaaa
     4921 aattcgttga gccttgcttt catggagtca gccgtttcca tgccttcgta tacatcggct
     4981 tcagcatagg gaacaggata gggcttctcc tgtaatattc ccaggtggcg ttccatcaaa
     5041 cggtgatcca ccattaaatg ggtatgcgga ttatatcgct gaatcgcttc tgctgtttgc
     5101 agggccctgc cgagcggact gctgtagata gtcttaaaga aatgttgctg aagttctctc
     5161 ccgagttcct cattcgcagc cttcccttct gcagataaag taccgtcagt ctgtccttgt
     5221 gcaatatttg ttgcgttttc aagggttact ccatggcggg caattatgag tttcaaaggt
     5281 ttcaaaagtt taagaggttt caaaggttaa aactacaact tcttaaaacg atgaacctct
     5341 taaaacctct taaacctctt gaacctttta aaccttttat tctaccgtga tcctgattcc
     5401 ttccgagtga gcggtaaact ccggcgcgta catgcactgg atactggtga tgccattgct
     5461 gaattcaccg gcatgggtgg cgaagagttc atactcgaat acataggttc ccttccgcag
     5521 gaagctgaag aagaaattgg tacttgcgtc tttggtactc tcataatagc ctagtccgcc
     5581 ctgccacttg taactgctca gtacattcac cggttctaag gcagaagccc tcatatcttt
     5641 catgtgcaca tattccatat cgcgatctac gcgcagttcc actcttactt taattttatc
     5701 gcccactttt atggcttctc cttctgttac gggcgtgagt accggaccac ggtcgctgtt
     5761 ggtctggatg aataattgct tcgacagttt caacggtgtt tgggccgtgg tgattttgtc
     5821 taggtcttcg aagtattgcc agtagacaga accccaggaa gaagaactgt ttagttgttt
     5881 agtcgttgag atgtttagcg gttgatttac actgattgaa atgtttccct gttccgggtt
     5941 gactttctgg gcttcgattg tttttttgaa atagccggtg ccggcttcgg cgcctgcatc
     6001 ggtggatttg aaggtggtat tacccagttt gatttccact accggttcat tggtcaacca
     6061 gttgctgcct tgcatgagta gggcataaca ggcttcggcc gtggccttgg tggtcttcca
     6121 gttattggtc tgtttgtttt tcagcaacca ggtctttagt tcatctacgg tggcctgatc
     6181 cttgcccgct tctgtaaaag cctcaatcag cagggcatgg gtttcgatag gcatttcgta
     6241 ccagaaccag ccataacgct gatctttcca gtagcggccg agttcttcat gggtgatgga
     6301 ggtctctttc agtgatttca aaatagccac cggtgttttt atatcaccgg tacgatacag
     6361 ggccagggcg atcatgccct gcatgtattt gctttgtttt acccagttca ccgccagttt
     6421 gccgcggaag aaatcgtagg ctgtttttgt tcctttggtg atttcataat ccgggaaaaa
     6481 actacgcata tagagatact ggatctcgct ataaccgggt gagtaagttt tgagattgac
     6541 tttgttttta atcagtccgt catagtcggc ttttattttt ttatcgagat aagggagggc
     6601 cagggtaaga atcgatttga gatagccttc ctgtccggga gcaatgccat tcagtttttt
     6661 aaggtgacct atgcccgtaa cgatgtactg ggtgatatag cggtcatccg gtccgccact
     6721 gaaccagaca aagccgccat tgggaccctg catttttttc agtttggcga atgaactgtt
     6781 cagcgcctca ctcattttca ccatatcaaa aagcagggcg atattctttt tctgctgggc
     6841 ttcagttttg gcttcgagta cccagggtgt ttcttcaagt aataccgctt tgagttcctg
     6901 gtttttctcc aggttgctca tcagggccgc ggtatctttt attttccatt gttcaaatac
     6961 ctgtttgatc ttcggactgg cactcgtgat ataactggcc agtgaattgg cgtagtaccg
     7021 gttccaggtt tgttcggcac agtcgtaggg atattccatc aggtagggca gggcctgtac
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaattccgga accagccatc
     7201 aatggaggtg ttggtgctgg catcaaacaa ttccagttga gcggtacccg tgagttcctt
     7261 atctgtaagg ttcacgatct tggtgctgaa ttccatctta tccccctcgc ggaggaagcg
     7321 gggcgcatta ggttgtacca tcagttcttt ctgcgttacg atctctttgg tgctatagcc
     7381 aaaggctgca tcctttgtat gggccagggc cataaatttc catttggtca gggcctcggg
     7441 catcgtgaat gagaattcaa tggcgccggt gctatcggtc tggagctcgg gcaggaagaa
     7501 ggcggtttcg gagaagtttt tgcggatggt aatcggctca attgtattcg aattattttt
     7561 aacttcttcc acttttaggt atggcacatt tgtagtaaat gaagtttctt tttcagcaat
     7621 ggccgcatcc atgccattct cttctcctct ttttaatgag gggtgggctg gtgtcattga
     7681 ggcctccatt gcattgcccc ctaacatata agtaaaacga cttgttgttt ttttgtcaaa
     7741 ttgatatttg aaattatcga tagccattgt gttgtcaatt agcatatcat aatttttttc
     7801 gaggtacttt cccgcttgtt gatcagcgct ccgatgtgat gaagttaaat cttcaaaatt
     7861 tgtactgtta ttccatgggt tttggagttt gtttaaaggc catggcatag gtgaattcca
     7921 gtaatgctcc acaaattgat ccagtgaagc gtcatacatt gctgctaata tttcagcggc
     7981 cactttctct ttcttatttc cgctgatccg cagtttccat ttttcttccg atccgggtaa
     8041 tgttttgtcc ctgaatgtgg cgtattctat gtccaatact ttattggacc agggtacatt
     8101 cgtaatatcc gtatattgga aaaaacggtt attcttaaca aaggcccaga ggctgctaat
     8161 gccaccccgg tcttcttcgt tgacgggggt atcaaaagtt cttttctcat tgcttaagcg
     8221 gatataggaa tagttgctgg ctttgtttat ttgctgccgg atcacaaata aattttcggc
     8281 ggaggttcct atattgaact tcgctgtttc tcccggctcc acaggcccca tctttttatt
     8341 cgtccataag tatgcggcgc ggtttagctg attgctcttt tcatctatca gttctgtata
     8401 ttcaacagac ttcacttcct ctccattttt tccggtagcc gtcacttcaa tcacataaaa
     8461 gcccggagtc agattcccac tttccactct ccactttcca ttttcgggca cactgtcact
     8521 ttttgtataa accgactctc ctgtttccca gtttgtatac tccgattcat tatcatattc
     8581 atccaccggg aatagtttca catattcttc cttgctcatc aggaactgat cgggtctttc
     8641 ccagtaccgt ttacggatca ggcgtttttc ttcctttagt ttatgaatga tcactttggc
     8701 ggtagcggga ataaattctc cattcatatt caccgtacgg atactgaggg ttttcagact
     8761 gtccgctggc aatacatcag gaatggtagt aaccagtttc atcgctttgt agctgacgga
     8821 tacggcctgt tctccgctac gggtttcgcc gttgatatcg gttacatcag catagacggt
     8881 atagtcaaat accggttcaa acttcttgtc aatcttcaga tcggggatgg cggtaaattc
     8941 aacattgaat ttgccgtctt tatctgtttt aatttctcca tgtgttatct ccatcgattg
     9001 ggccggaggt tgccaccaac gccttaaaag ccagggataa aggaaacggg cctcccgcac
     9061 cacccggtat tttaccatgg ccccatcaat attgttaccc gcataggctt tggccgtacc
     9121 ggtgatggtt attttatcat ttactttata agtgcccttg atgggttcat attcaacctg
     9181 aaatttcgga cgtttatatt cttctacttt gaacccggtt tgtgcatttt ctcctttagt
     9241 ataaatgctg aaatctccat tcaggctccc ttggggcaat tggaatttgc cattgaagga
     9301 accaaactca ttggtcttca gcttcaggct gtctacgtct ttgtcattgg catcccgcag
     9361 gtaaaccgtg gtggtgtacc cggcacggat atccgctttt ttaatatcca tattcagggc
     9421 cagtacaatg cctttgaggt aaagcgtttg tcccggacga tatatggccc gatcagtgaa
     9481 aagatgtatg gctgttttgc catgcgtgtt ttggtcctcg ttgttccggt aatagtaata
     9541 gtcattctgg tattcattca tgaacaaccg gtcattgtca tgtttgatat ccaggaggaa
     9601 tgaataattc cagtagttat tttcttcttt tgccggtcgc tccattttaa agaaaccatt
     9661 ggcatctgtg gtataggctt tgcctcttaa ttttatgttt tttgattgct tctgatcata
     9721 tttctgttcc catacctgta tgcttgcatt ggccaggggc tgaccggttt cgcggtgcag
     9781 gacaaaatag tcctggtctg tgtttacata actgatcccg gatacataaa agctgcgggc
     9841 ccccaacaaa ctcttcgcat ttttaaaatc ggcattgtct gaagccagta agatgtattc
     9901 acctgctggc aggccatcta tttttatctc cactttatgt tcctgtaagt cattggtagc
     9961 cggcaatggt tgttgccagc tcttcatgac tggtgcgctg accagggtgg gccagtattt
    10021 ttcattatat tgattttcca gctgggtgcg gagtttttca tcggagcgga tgagccttaa
    10081 gtgcagggta gttacatttt tataggtaac cagggagcgg aagggcatgt ccggactgtt
    10141 tactttctcc agctgaaatt caaaactgct tctgttcagt tcgaccaaga gatttccggc
    10201 attgatcttt ccttcagagg aatctttttg taattgcagt ttttcagcaa tttcttttgc
    10261 ctttatccgg gcatattgga aagtagtatc gccgtgaggt ttgtaatcat tggcctgctc
    10321 attataatat agtgccaata aataccaggc ctgggcggct gccgggtggt tttcgtactg
    10381 ccgggcaata tggttgatgc tgttaaagta cagttggtct ttatcggggt gggtggagtt
    10441 ttcttttaca aatcccagcc gtttcagatc cgcatctatg agtgcatccg gtttagtgtc
    10501 gttaagatgg aaggcaatga gttcctggta gatcaacagg gctttgtgtt gaagcgacaa
    10561 ggagtctttc gtcgtgaatt ttctttttat aaaatcagct gccgggtcaa aggcgctgga
    10621 ctggtcaatt tcaaaagcat aggcgggttt agtgatatcc ctttcatcat tttcaaaata
    10681 gttcaatgca tggtgagcca gcaagtcgta cagtgtggga cgcaggtggc gcacattccc
    10741 tttaatgatg attgcattga acggatccag ttttgtctgt tgcagaaggc gggtgtcttt
    10801 caatgattgc aggtaaagct cactgatctt tttgtgaaaa tcctccgctc cccaggtggc
    10861 gatatccgtt ttattaaatt tcgccgtctg cgtgcggtcc attaattccc agcggttccc
    10921 ctggtagtaa ttccagtaca tgccggctat aatattttta agtagagcag ttacgggttc
    10981 tttgctggtc ttcagctctg tttccatttc ccggatggaa aggacatcgt tgtcttcccg
    11041 ggtgtcggac tgcaagccgg atatgtataa taaagatttt atgacctggg catcctgctt
    11101 ttctttttta gccagggcat aaattttctt tacttccgtc aatgcggatt ggggcagtcc
    11161 tttctggctg aactgttcca ctttcttcca ttctttatcg tagtttttga ccggtgtctg
    11221 gctctggctg ttcataacaa aaaagcaggt aaaaaggatg gttaggataa ggttatgctg
    11281 gagtcgcatt ttcatgctgg tttattttag tataaaggta gtgattatgc gtcagcgccc
    11341 ccttcgggag acactgacgc taatacaaga tgcaatgctg gttatgattt cataatcctg
    11401 caacaggaaa tccgtgtcag gtaaggaatg acgcagaatc ctgcatttct ccgcaaattt
    11461 tgcccgggtc tttaacattt tgttaccccc ttgttttggc acgctggcac gggtttggaa
    11521 atactcttta cagaaccgaa ttattt
//
LOCUS       DAOPMW010000067        11473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_5971_length_11473_cov_6.363812, whole genome shotgun sequence.
ACCESSION   DAOPMW010000067 DAOPMW010000000
VERSION     DAOPMW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5971_length_11473_cov_6.363812"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PLU11_05305"
     CDS             <1..1835
                     /locus_tag="PLU11_05305"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN58563.1"
                     /translation="FNDQRNSMALYTNAYGVQRDLLNFDDMYTDLDWDGLWKVRTTRT
                     DSGWYAEMAVPWQTLRYPREKDSIQQWGFNIYRNRRLSNEISAFSAYPRSFSFLRMAY
                     SGKLTNLEPPPPGRNLRIQPYVLTSYDRYKNNTSIMDPEKTAFRYGGDIKWAITPHSI
                     LDLTFNTDFAQADADRQVNNVTRFSVFFPERRQFFLENASLFGTGVAPSEDLSGGSMR
                     IQPFFSRRIGLDDSGNPIPIDAGGRFVYRSAKNNIGAMLMHQQGSSNMPATDFFVGRY
                     SHNLGRLNRIGGLVTARNNSQGHNIVSTVDGFFRLSESHQLNMMVSHSSTSATGKQGL
                     SGSAQYYYVNNQWKIWWTQSFVSKNFNPEMGFVSRNDVIATTPGVFWYYRGKNLPFPK
                     LIRAFEPGAWVEMYHQASTGKLTERTVSLNPVWFNFQQGGFIGYIVNHSFQRLTETFS
                     PVGIDILAGDYNYFNHQIYYSTDPSRKISGGLDLTFGDYFNGKLTSTNTRLQLSPSPH
                     FSLLAQYNRLHFDEVGSPSISKKTDLYTISGRFALNPRMQLIGFYQLNSENKLGNYNL
                     RFSWEYQPLSYIYLVFNHRGFDNVLAKRQTEDHSIIKLSYLKQL"
     gene            2060..2338
                     /locus_tag="PLU11_05310"
     CDS             2060..2338
                     /locus_tag="PLU11_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HPN58564.1"
                     /translation="MANETGTATGTDSYTGQFKNGYPDGKGKYTWKNGEYKGKFENPY
                     TTEMLTNNFTSISVKKMNTLISEITFTVKNTTAGGSTNNQPVLPKSEM"
     gene            2426..2548
                     /locus_tag="PLU11_05315"
     CDS             2426..2548
                     /locus_tag="PLU11_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58565.1"
                     /translation="MYPFSAIITFERARSVQQVAIAKIKLNENGNWMIRVDVEQ"
     gene            2765..3853
                     /locus_tag="PLU11_05320"
     CDS             2765..3853
                     /locus_tag="PLU11_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58566.1"
                     /translation="MKKLYLFLLLTTPLCVFAQKNVDLDKYRFTVQYRALPQVQLDST
                     YRTYHVEIESTRLMDSFMDDLSPEKSVRLEGWRKLEQDGHLGIQVKLGDLLPESFSVK
                     ERTETVKNGNGIVTGSRTYYHQEVVYSFSANAIISDYKGMHIMDQELASRQNKQIYKS
                     PEFTIKHLAEGYFLVNSMSVTRDLYRNAVTRSMHTLSDRITRNFGFDEVAVNDYMWII
                     DSRKHPEYSAWRNAFTQMSEVLYNMDANTPITGVREKLQPVISYFEGIRKRYTSTKKH
                     DRKIRYATYFNLAVLYYYLDDPQSMMREANGLVLNDYDSRDGKGFEETAAWLKNQFQQ
                     NNIYTRHFPVNPGSYRGPLEKKDISSIN"
     gene            complement(3997..5106)
                     /locus_tag="PLU11_05325"
     CDS             complement(3997..5106)
                     /locus_tag="PLU11_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58567.1"
                     /translation="MNHKPVFYLLSLLSFFSCTKNREQETRSFSMGVTPWPADFTAVE
                     QARAYHFINTECDLVSHHFDEGVPYKEALSMLAMPLKLQESLQFRKANTLPGKRILLS
                     VSALNLTRKERAGYFDEGIVADSVKNRWAAMAFDSPQVVTAYVNYVSWLVDQLQPDFV
                     NYGVESNLDSWDPVKFARYKQFLSQVYPRLKIKYPGLPFFVSFMVYEVPQALTLSREL
                     LPYTDYVAISAYPYSSVSSSANGNTDPALFPSDYFSRFADLDANKPFAVAETGYIAED
                     LSIPSFSLQKTGTPAWQQAYLEKLCAFAQSRKARLFVWFCYKDYDAGSQTLLGAGLYQ
                     DLFGLWEDTGFYDEAGQPRPALTSWRQWMQRPARH"
     gene            5443..8022
                     /locus_tag="PLU11_05330"
     CDS             5443..8022
                     /locus_tag="PLU11_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN58568.1"
                     /translation="MRTHKYVIFLLFFLFFIFAFFLWEFKSINVNNRQNVDAVETTTR
                     FSKNLNQLRFELLKTDNNISSGISKPVYRLHNDFKADSTSLIRLFNETAFTKTEEPSL
                     NNSLVTLRKKVEEKLGLQSAFVNYNPTDSMAGLVYNRQNEAVNRSIQSGIASVNNLQQ
                     KLFSVQIGKVEDTYTKQYLFALGFAILSFLIFYFLLTGLNSHIQRRKSAEEEAIINEE
                     KYKNIIDESGAIIFTTNMEGRFTFVNEMACRVTGFAQEELLKKSYIDLVHPVWQEQLL
                     NFYSDALRKGDLETQIKFPITCKSGEMKWVEQTAVIVFQQGQPMGFHCVTKDITESYQ
                     LEREKKYLYQLFQSIMDNSPQAIFIKSTEGRYIHVNKQFCELLMLTELEIVGKTDDEL
                     FGKSLTLHYRNKEAQVIKTQTPSRLRQNFNSKKGIRTFSIELFPLFDHNNEVLGVSGI
                     ASDITDAVQNQRNLISALKRAESAEKHTQSFLANMSHEIRTPLNSIIGLSYQLLKSDM
                     RQKEHGFVRTIHAASEHLLVLINDILDTSKIEAGKLGLDQTSFSLRELIQLMVELLRQ
                     KAEEKNIRIITEIDSNLFDSHIGDPHRLKQILLNLLTNAVKFTPKGLIRITCKVEEDE
                     AKQQSIALTIIDTGIGIEQEYIDRIFTKFSQEDKSTTRNYGGTGLGMFITKSLVELMG
                     GTISVSSQKEIGTHVKINLSLPKNLVPVIQLPAKATTDTSILNNLHILVVDDNEMNRL
                     VASTVLENHGIIVSEAVNGADAIRKLEKESYDLILMDLQMPVMDGLEASLKIRKDLGL
                     SVPIVALTASALNEESHRCLDAGMNDFIIKPFKEEEMVQVLIRNIRLDHRAELNDPAR
                     KIS"
     gene            complement(8109..9701)
                     /locus_tag="PLU11_05335"
     CDS             complement(8109..9701)
                     /locus_tag="PLU11_05335"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN58569.1"
                     /translation="MHTRFLIGLLFISLFSFAQNLKSGGRLKPEQAIMDIRHITIALD
                     VDPEKKTIDGYTETDLVLAEPSGVLLFDLWHEMKVSKIWVNGKAASFTHTPDDLVRIQ
                     AAGILPAGKVKVKIAYGGTPGIAVRPPWTGGFQWEKDAKGNPWIAVSCQSEGAKVYFP
                     CKDHPSDEPNEGAEMIITVPKGLVVAAPGLLQKQNTKKNKTTWHYKTNYTISNYCLVF
                     NVGNYKVAKRVYTSVTGKKIPMEYYVLEENLGKAEKLLDLFEQSSRILEKYFGEYPWA
                     NERIAASETPHLGMEHQTNIAYGNKYRYEKLGSYDFDWLLHHEYGHEWWANKVTNRDW
                     GHMWIQEGICVFGDAMATRELAGEEAYLQRMQQTALNTQNKFPVVRGDEVDSDSAYHG
                     DIYGKGAFFMHTLRFVIGDEVFFPTVKRLSTEPKYVYDNTVVTADVEQLFSSASGKSL
                     QPLFKLFLYTTDKLEISVRQTGLDKYMIRLLNIDMPLPLEIKTDEGIKKIMVDKKGAS
                     VSSKTFVQVDPRVFYLKKLIYE"
     gene            9882..10343
                     /locus_tag="PLU11_05340"
     CDS             9882..10343
                     /locus_tag="PLU11_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58570.1"
                     /translation="MAVFILSCSPSKRYNTPEELGKTVFELFQKKETSQLKALLISKS
                     EIETAFGSSPQNQSLTDEERKVFINSTYERNKEIGEGFIDFYTGGDPAATKMLASGKL
                     EKTTADIRDDNGTSIAWIVNHFKVEGKTLELNLRAAKVNDYWKLLSWIEVK"
     gene            complement(10368..11267)
                     /locus_tag="PLU11_05345"
     CDS             complement(10368..11267)
                     /locus_tag="PLU11_05345"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HPN58571.1"
                     /translation="MIRIVILSAMILISFSLAGQKVDWKKIQEAHPDKILLNGERQPV
                     PVLLLGTFHFGYPNLDGHKTDSSKYIDVKSLVRQKEMEELAAVIMRFKPTRVYVESSD
                     EGWIDSLYAAFRNTQYKLGRNEIFQLGFRVAGLAGHKKVYSVDTWPFSSEFGEKYPWI
                     DSLWSHNTPVDSVRDQYWAAKYKSWYDAGDAQEINMTMLENFLNMAEPATLQRMHGHY
                     LASGFNTAEEVNGNGQQGPDALSIWWYNRNLRIFNNILRTKPGPEDRILVLFGNGHMP
                     ILRHCFYSSPEFRVVELKTLLKK"
BASE COUNT         3390 a         2710 c         2396 g         2977 t
ORIGIN      
        1 gttttaatga ccagcggaat tccatggctt tatatacaaa tgcgtatggc gtacagcggg
       61 atctcctgaa ttttgatgat atgtacactg acctggactg ggacggactc tggaaagtca
      121 gaaccacccg tactgactcg ggctggtatg cagaaatggc ggtgccctgg caaaccctgc
      181 gatatccccg ggaaaaagac agtattcagc aatggggatt taatatttac cggaaccgtc
      241 gtttatctaa tgaaatcagc gctttctccg cctatccccg ttccttttct tttttacgga
      301 tggcctattc tggtaaactc accaacctgg aaccgcctcc tcccggacgt aacctgcgca
      361 tacaacctta tgtacttact tcatacgacc ggtataaaaa caataccagt attatggatc
      421 ctgaaaaaac cgcattcagg tatggtggcg atatcaaatg ggccatcacc ccgcatagta
      481 ttctcgatct gactttcaat accgattttg cccaggccga tgccgaccgc caggtaaata
      541 atgtaacccg gttttcggtt ttctttcctg aacgtcgtca gtttttcctg gaaaatgctt
      601 ccctctttgg aaccggtgtc gccccttcag aagatctttc cggaggcagt atgcgcatac
      661 aacctttttt cagccgccgg atcggactgg atgatagtgg taatccaatc cctattgatg
      721 caggtggacg ttttgtatac cggtcagcca aaaacaatat cggtgccatg ctgatgcatc
      781 agcaaggctc ttccaacatg cccgccaccg atttttttgt aggacgatat tcccataacc
      841 tgggaagact gaaccggatc ggagggttgg tcacggcccg gaataacagc cagggacata
      901 atattgtcag tactgtagat ggattcttcc ggctgagtga atcccatcag ctcaatatga
      961 tggtctctca ttccagtacc agtgcaacag gaaaacaggg actctccggc tctgcccagt
     1021 attattatgt caataatcaa tggaaaatct ggtggacaca atcctttgtt tcgaaaaatt
     1081 tcaatcctga aatgggcttt gtttccagga atgatgtgat cgccaccact cccggtgtgt
     1141 tctggtatta ccggggaaag aatttaccat ttcccaaact gatccgggcc tttgaaccag
     1201 gtgcctgggt tgagatgtat caccaggctt cgacggggaa gctcaccgag cgtaccgttt
     1261 ctttaaatcc ggtgtggttc aattttcaac aaggtggttt tatcgggtat atcgtgaatc
     1321 acagtttcca gcgactgacc gaaaccttct ccccggttgg tattgatatt ctggctggtg
     1381 attataatta ctttaatcac cagatttatt acagcaccga tccttcccgc aaaatcagtg
     1441 gaggacttga cctcacattt ggtgattatt ttaatggcaa actaaccagt acgaatacca
     1501 ggctgcaact ctcgccttcc ccgcatttca gtctgctcgc ccaatacaac cggcttcatt
     1561 ttgacgaagt gggctctcct tccatcagta aaaaaacgga cctgtacaca atttccggtc
     1621 gctttgcctt aaacccaagg atgcaactga tcggatttta tcagctgaat tcagagaaca
     1681 agctgggaaa ctataacctg cgtttctcct gggaatatca acccctgtcc tatatttatt
     1741 tagtattcaa tcaccgggga ttcgacaatg tacttgcaaa aagacagacc gaagatcatt
     1801 caattatcaa actgagttac ctgaaacaac tttgaaaaac ggacttataa tgatttccag
     1861 cttgggttga taccggatat agaacaaaca tcttttggta tcaggcatca caaatagagt
     1921 agcttcaaaa aaaaggaaat atgaagccgt ctatacccct tttactttct ttcttttttg
     1981 ttttaaacac aacgggtcag gaatgtaacg tcatgctgga ttcactaaag gaagctacat
     2041 aggagaatac agaaagggat tggccaatga aaccggaacg gctaccggaa cagacagtta
     2101 taccgggcaa tttaaaaacg gctatccgga tggtaaagga aaatacacgt ggaaaaacgg
     2161 ggaatacaaa ggaaaatttg aaaaccccta tactaccgaa atgctgacga ataatttcac
     2221 cagcatcagt gtcaaaaaaa tgaatacact catttccgag atcacattca ctgtcaagaa
     2281 cacaacagcc ggaggctcaa ccaacaacca gccggtactc ccgaaatcag aaatgtaaaa
     2341 cattcagtta gtccagggaa attataccga gttggtctat gacagcacct cgcaagtcac
     2401 gaatcgctat actttcagaa acatcatgta tccattcagt gctatcatca ctttcgaaag
     2461 ggcccgttct gtacaacagg tggcgattgc aaaaattaaa ctcaacgaaa acgggaactg
     2521 gatgatccgg gtggatgtag aacagtgaag gaactggttg ttaagtttaa aagtaaaaag
     2581 gttgacaagg aagattcact tttgggacat cctgactcct gaagcattgg gatgacatct
     2641 gacaactgtt ttcgtgtttt ccctcgtgta tttacgacaa acgcacctgc ccgatacaat
     2701 attctgctac tgatcatgta ctatttaagc tttgcttccg ttttttacag acaaaatcct
     2761 gactgtgaaa aaattatacc tgtttcttct gctgaccacc ccgctctgtg tttttgccca
     2821 gaaaaatgtg gatcttgata aataccgctt tacggtacaa tacagggcgt tgccgcaggt
     2881 gcagctggac agcacctatc gtacctacca tgtggaaatt gaaagtacac gcctgatgga
     2941 ttcttttatg gatgacctgt cgccggaaaa atcagtcaga ctcgaaggtt ggagaaaact
     3001 ggagcaggac ggacatcttg gtattcaggt taaactggga gatcttttac cagaatcgtt
     3061 ctccgtgaag gaaagaacgg aaaccgtaaa aaatggaaac ggaattgtaa ccggcagccg
     3121 gacttactat caccaggaag tggtgtatag cttttccgct aatgccatta tctcggatta
     3181 caaaggcatg catatcatgg atcaggaact ggcctcccgc cagaacaagc agatttataa
     3241 aagtccggaa ttcaccatca agcacctggc agagggatat ttcctggtaa actccatgtc
     3301 tgttacccgt gacttgtatc gcaatgcagt aaccaggtcg atgcatacgt tgagtgaccg
     3361 gatcacccgg aacttcggtt ttgatgaagt agcagtaaat gattatatgt ggatcattga
     3421 cagccgtaaa catccggaat atagtgcctg gcgcaatgct tttacgcaaa tgagcgaagt
     3481 actttacaat atggatgcca ataccccaat caccggcgtc cgtgaaaaat tacaaccggt
     3541 gattagttat tttgaaggca tccgtaaacg atatacatcc accaaaaaac atgaccggaa
     3601 aatccggtat gccacctatt tcaatctggc cgtattatac tattacctgg atgatccaca
     3661 gtccatgatg cgggaagcca acggactggt gttaaacgat tatgacagcc gcgatggaaa
     3721 gggattcgaa gaaaccgcgg cctggttaaa aaaccaattc cagcaaaaca atatttacac
     3781 ccggcatttc ccggtaaatc ccggttccta ccggggacca ctggaaaaaa aagacatttc
     3841 cagcattaac tgataaggtt gtcattaaac taaaaaaggg gtaatactaa atacctcttt
     3901 atttaaaaaa gcgaaacact cctcacctgc ttagaagtat tttatgctga gtagtcctaa
     3961 acatgtgata aatagtatgc cgaaaataaa aataagtcag tgtctggcag gtcgctgcat
     4021 ccattgccgc cagctggtca aggccggcct tggctgcccg gcttcatcat agaaaccggt
     4081 atcctcccac aggccaaata aatcctggta gagccctgcc cctaacaatg tctggctgcc
     4141 ggcatcataa tccttgtaac aaaaccacac gaaaagacga gcctttcgcg attgagcaaa
     4201 agcacaaagt ttttccaggt aggcctgctg ccaggcgggt gtaccggttt tttgtaaaga
     4261 aaaggaaggg atggataaat cctcagctat atagccggtc tctgcaacgg caaaaggttt
     4321 gtttgcatcc agatccgcaa aacgtgaaaa ataatcagac ggaaataagg cagggtccgt
     4381 attaccattg gccgaagaac tgaccgatga ataaggatag gcactgatag ccacataatc
     4441 cgtataaggc aataactccc tggaaagtgt taaagcctgt gggacctcgt acaccatgaa
     4501 actcacaaaa aaaggtaaac cgggatactt tattttgagc cgtggataaa cctgggacag
     4561 gaattgttta taacgggcaa actttacagg gtcccaacta tccaaattgc tttccacccc
     4621 ataattcaca aaatcaggtt gcaactgatc caccagccag ctgacataat tcacataggc
     4681 cgtaaccact tggggggaat caaatgccat tgcggcccac cggtttttca cactatctgc
     4741 cacaatacct tcgtcaaaat atcccgccct ttccttccgg gtcagattca acgctgatac
     4801 actcagcagt attcttttgc cggggagcgt attggctttc cgaaactgca ggctctcctg
     4861 cagcttcaag ggcatggcca acattgaaag cgcctctttg taagggaccc cttcatcaaa
     4921 gtgatgtgag accagatcac attctgtatt gatgaaatga taggctctcg cctgttccac
     4981 tgccgtaaaa tcagccggcc agggggttac ccccatagaa aaagagcggg tttcctgctc
     5041 cctgttcttt gtgcaggaaa aaaaagaaag caggctcaag aggtaaaaga ccggtttatg
     5101 attcatgagt gactgagatt aattaaacaa tagcaggagc taccctgtat tctgcagccc
     5161 gaaactaacg gggctgttat aagtaaatct gttaaaaaaa acaaatcctg actactacct
     5221 gacgatgcca gaatcccata ggttactgaa aaacagggct atttgtacgt agaaaaattc
     5281 cgcggatact tatacggaat atccgtagtc cgaaatttac cactatgccg gactaagagg
     5341 aaaccaataa attgatttta atcctgcgaa catactggca aataagcaac aaaatgatca
     5401 actttaatcg ttagtagcac ataaatccga atccctaccg caatgagaac ccataagtat
     5461 gtcatattct tactcttttt cctgttcttc atttttgcat tttttctgtg ggaatttaag
     5521 agtatcaatg taaacaaccg tcagaacgtt gatgcagtgg aaaccaccac ccgcttctcc
     5581 aaaaatctga accaactccg tttcgaacta ctgaaaacgg ataacaatat ttcatccggt
     5641 atcagcaaac ctgtataccg gcttcataac gacttcaaag ctgattctac ttcgctgatc
     5701 cgtttgttta atgaaactgc ttttactaaa acagaagaac cctctcttaa taacagcctg
     5761 gtaacactcc ggaaaaaagt cgaagaaaaa ctcggcctgc aatctgcatt tgtaaactac
     5821 aatccaacag acagtatggc tggcctggtt tacaacaggc aaaatgaagc agttaacaga
     5881 tccatacagt caggaatagc ttctgttaat aacctgcaac aaaaactctt ctcagtacag
     5941 atcggaaaag tggaagacac ctataccaaa caatacctgt ttgcactggg ctttgcaatc
     6001 ctttccttcc tgattttcta tttcctgcta accggattaa actcgcatat tcaacgtcgg
     6061 aaatccgcgg aagaagaagc catcattaat gaagaaaaat acaaaaatat tattgatgaa
     6121 tcaggagcca tcatatttac aaccaatatg gaagggcgtt ttacgtttgt caacgaaatg
     6181 gcttgccggg tgaccggttt tgctcaggag gagctcctga aaaaatccta tatcgacctg
     6241 gtgcatccgg tatggcagga acagctcctg aacttttaca gcgatgccct gcgcaaaggc
     6301 gatcttgaaa cacagatcaa attccctatc acttgtaaaa gcggggaaat gaaatgggtg
     6361 gaacaaaccg ctgtaattgt atttcaacaa ggccaaccaa tgggattcca ttgtgtcaca
     6421 aaagatataa ccgaaagtta ccagctggaa cgggaaaaga aatacctgta ccagctcttt
     6481 cagtcaatta tggacaacag cccccaggcg atctttatca aatcaacaga aggccgctat
     6541 attcacgtca ataaacaatt ctgcgaactg ctgatgctga ctgaactgga aattgttggt
     6601 aaaacagatg atgaactatt tggcaagagt ctgaccctgc attaccgtaa taaagaagcg
     6661 caggtcatta aaacacaaac accaagccgg cttcgtcaga actttaacag caaaaaagga
     6721 atccggacat tctctattga acttttcccg ctttttgatc ataataatga agtgttgggt
     6781 gtttccggta tagcgtcaga tatcacggat gccgtacaaa accagcgtaa cctgatcagc
     6841 gcacttaaaa gagccgaatc ggctgagaaa catacgcaaa gcttcctggc caatatgagt
     6901 catgagatcc gtacgccatt aaattcgatc atcggactca gctaccagtt actcaaatcc
     6961 gatatgcgac agaaagaaca tggttttgta cgcacaattc atgcagcatc tgaacatttg
     7021 ctggtactga tcaatgatat tctggatacc tcgaaaattg aagcaggcaa actgggcctg
     7081 gatcagacgt ccttctccct gcgtgaactg atccagctga tggtggaatt gcttcgccag
     7141 aaagccgaag aaaaaaatat ccggatcatt accgagattg acagtaacct gtttgacagt
     7201 catatcggtg atccgcaccg gttgaaacag attttactga acctgctgac caatgccgta
     7261 aaatttacgc ctaagggact tattcgtatt acctgtaaag tagaagaaga cgaagccaaa
     7321 caacaatcca ttgcgcttac gatcattgat accggtattg gtattgaaca ggaatatatt
     7381 gaccgcatct ttaccaaatt ctcacaggaa gataaatcca ccacccgcaa ttacggcggc
     7441 accggactgg gtatgtttat taccaagagt ctggtggaac tgatgggcgg cacaatttct
     7501 gtcagcagtc agaaagaaat cggtacacat gtaaagatta atctcagtct gccgaagaac
     7561 ctggtacccg ttatacagtt accggctaaa gcaacaactg atacaagtat tcttaacaac
     7621 ctccacatcc tcgtcgtgga tgataatgaa atgaaccggc tcgtggcttc caccgtattg
     7681 gaaaatcatg gaatcatcgt aagtgaagcc gtcaatggcg cagacgccat acgtaaattg
     7741 gaaaaggaaa gctatgacct tatcctgatg gacctgcaaa tgccggtaat ggatggcctc
     7801 gaagcatccc tgaaaatcag gaaagacctc ggactctccg taccgatagt tgccctgacc
     7861 gccagtgccc tgaatgaaga aagtcatcgc tgcctcgatg caggcatgaa tgactttatc
     7921 atcaaaccat tcaaggaaga agaaatggta caggtcctga tccggaatat caggctggat
     7981 caccgggctg agctcaatga tcctgcccgg aaaatttcat aggtcaacta cacaatgaca
     8041 atactcacaa tcaggtaacg tatgaacacg cttcacttac tatcaatcga taaaccaacg
     8101 aagaactttt attcatagat cagtttcttc agatagaaca cccggggatc cacctgtaca
     8161 aaagtcttgc tgcttaccga agcccccttc ttatccacca taattttctt aataccttca
     8221 tctgttttga tttccaatgg aagcggcatg tctatattca gcagccggat catgtattta
     8281 tccaggccgg tctgacggac actgatctcc agtttatccg tggtatataa aaataattta
     8341 aacaatggct ggagtgattt gcccgaagct gaactgaata actgttctac atccgctgtc
     8401 acaaccgtat tgtcatagac atattttggc tcagtggaca gtcgttttac tgttgggaaa
     8461 aaaacctcat ccccgattac aaaacgcagg gtatgcataa aaaatgcgcc cttgccatag
     8521 atatccccat gataagcact gtctgaatcc acttcatcac cacgcaccac cggaaattta
     8581 ttctgcgtat tgagtgctgt ctgctgcatg cgttgcaggt aagcttcttc tccggccagt
     8641 tcacgggtag ccatggcatc cccaaataca cagatccctt cctggatcca catatggccc
     8701 cagtcgcggt tggtcacttt attcgcccac cattcatgac cgtattcatg atgcaggagc
     8761 cagtcaaaat catagctgcc cagtttttca tatcggtact tattcccgta cgcgatattc
     8821 gtctggtgtt ccatacccag gtgtggcgtt tcagaagcgg caatccgctc attggcccag
     8881 gggtactccc cgaaatactt ttcgaggata cgactggatt gttcaaacag atccagcaat
     8941 ttttccgcct tccccaggtt ctcttcgagc acataatact ccatcgggat cttcttcccg
     9001 gtaactgaag tatacaccct tttggcgact ttataatttc ccacattaaa taccaggcag
     9061 taattactga tggtataatt tgttttataa tgccaggtgg ttttattttt tttggtgttt
     9121 tgcttttgca gcaaaccggg tgccgccacc accagtccct ttggaactgt gatgatcatt
     9181 tcagctcctt cattgggttc atcactgggg tgatccttgc agggaaaata gactttggcc
     9241 ccttcactct ggcagctgac ggcaatccag ggattcccct ttgcatcctt ctcccattga
     9301 aaaccaccgg tccatggcgg acgtaccgca ataccgggag tacctccata agcaatcttc
     9361 acttttactt ttccggcagg taaaataccg gctgcctgga tccgtaccag gtcatccggg
     9421 gtatgcgtga agctggccgc ctttccgttg acccagatct tagacacttt catttcgtgc
     9481 cacagatcaa ataacaaaac cccgctgggt tctgccagca ccaggtcggt ttctgtataa
     9541 ccatcaatgg ttttcttctc cggatccaca tccagggcaa tagtaatatg ccggatatcc
     9601 ataatggcct gttcgggttt cagccggcca ccggatttga gattctgggc aaaagaaaac
     9661 aaggaaataa acaaaagtcc aatcagaaaa cgggtatgca tgggaactgt tttggggaag
     9721 ttacagcaat tccggcaact gggaaaccag gaaaataaat ccgtgtggta taatgcagtc
     9781 tggcggctgc cggagtaaat tgcattaaaa ttctttatta ttcaatcaat gccatgcatt
     9841 ttattaataa tgttacccgg actgtatgcc ccatactgtt gatggcagtt ttcatactat
     9901 cctgcagtcc ttccaaaagg tacaacaccc cggaagaact ggggaagacg gtatttgaat
     9961 tattccaaaa aaaggaaacc agccagctga aagccctgct catcagtaaa tccgaaattg
    10021 aaaccgcatt tggcagcagt cctcaaaacc aatcgctaac ggacgaagaa cgtaaagtct
    10081 ttattaattc cacatatgaa cgaaacaagg aaatcggcga aggctttatt gatttttaca
    10141 ccggcggcga tccggccgca acaaaaatgc tggccagcgg caaactggaa aaaaccactg
    10201 cggatatccg ggatgacaat gggacttcta ttgcctggat cgtcaatcat tttaaggtag
    10261 aaggcaaaac gcttgaactg aatttacggg cagccaaagt aaatgactat tggaagctct
    10321 tgagttggat agaagtgaaa tagccaatcg ctaatatgcc aattgattca tttttttaac
    10381 agcgttttta attccactac tctgaattcc ggagacgaat aaaaacaatg tctgagaata
    10441 ggcatgtggc cattaccgaa taaaacaagt atacggtctt ccggtccggg ttttgtccgc
    10501 aggatattat taaaaatccg caggttccgg ttataccacc agatggacaa cgcatccggc
    10561 ccctgctgtc cgtttccatt cacttcttcc gctgtattaa atccggaagc caggtaatgt
    10621 ccgtgcatgc gctgcagcgt agccggttca gccatattga gaaaattttc cagcatagtc
    10681 atgttgattt cctgtgcgtc acctgcatcg taccagcttt tgtattttgc cgcccagtat
    10741 tgatcccgca ccgaatctac cggcgtatta tggctccata aggaatcaat ccacggatac
    10801 ttttctccga attcactgga aaatggccag gtgtccactg aatagacttt cttatggcct
    10861 gccaggcctg ccacccggaa gcccagctgg aaaatttcat tccgccctaa tttatattga
    10921 gtatttcgga aagccgcata aagggaatca atccagcctt catcactgga ttcaacataa
    10981 acacgggttg gtttaaaacg cattatcaca gcggccagtt cctccatttc cttttgacgg
    11041 accagtgatt tcacatctat atattttgat gaatcagttt tatgaccgtc aagattcgga
    11101 tagccaaaat gaaaggtgcc gagtaacaat actggcacag gttgacgctc tccgttcagc
    11161 aaaattttat ccggatgagc ctcctgtatt tttttccagt ccactttctg accggctaat
    11221 gaaaatgaaa ttaaaatcat cgctgataaa atgacaattc tgatcattgc ttatgtttta
    11281 ggagataaaa atccactgct ttcatcgcct tttaaaatcc gatctgcata cccgtatttt
    11341 ttgtctgcca aaaaagatca gctcccggca tgcagataaa aagctgcgtt tcgcagacaa
    11401 cccactgatt tccgctcggg cttcagtaga tttgatggat gagaaacagc acgctcctcc
    11461 tggtctatgc cgc
//
LOCUS       DAOPMW010000068        11418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6016_length_11418_cov_7.324122, whole genome shotgun sequence.
ACCESSION   DAOPMW010000068 DAOPMW010000000
VERSION     DAOPMW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6016_length_11418_cov_7.324122"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..173
                     /locus_tag="PLU11_05350"
     CDS             3..173
                     /locus_tag="PLU11_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPN58572.1"
                     /translation="MSKEDYSGKSIKQLAKNYFEGSFSNVVSYLVKENNISVDELELL
                     LKQIKKQQNQSK"
     gene            170..3064
                     /locus_tag="PLU11_05355"
     CDS             170..3064
                     /locus_tag="PLU11_05355"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN58573.1"
                     /translation="MNPLLNYGIQVIIVSGLLYSYYHFVLRNKKFHRYNRFYLLGAAV
                     LSVLIPLLNIPVYFSEADTESSIVYNTLRVFATTETDEAVTIYAGIPEKESWFTLTHI
                     FQLVYFTIAIVVLLRVIFSLMKIRRIIRQHPVEKIENISFLNTDEPGTPFSFFRWLFW
                     NRKIELNSEKGEQIFRHELFHIEQKHSLDIIFLELLTVAGWFNPFFHLMKRETKAIHE
                     FLADQFAVTENNRWDYAELLLMQVLNTNQRLVHPFFHNQIKRRIAMITSPQKTSHQYL
                     RKLMVLPVTAVAVILFAFNYKNSNYNLPETESGEKQTIIIDVSHGGNDPGAIGADGTK
                     EKDLVLAIAQKIRSLNQNGNYNIVLTRDGDFLPELKSRVSTGNDQAPALFISLHINSS
                     PDKQKNGIEVYIPKNENKHFKESKAFGSILIGKLSENYEAGPNILQRSQTIWVLENSN
                     CPATLVEIGYITNEKDLYHIRNTNNQENIAKKILEAIYGYNIQDQDKKVNYSSSITND
                     TTIKPTRKMATFDQMVIVSDSIILKSFPSKALKDKKPEAIVFMNGKRIPKSEFVNKTI
                     LCDSFEYYDAGDKKAIELYGPEAKDGVMIIHNGKITDTEFELKEANKPEEDKIFSKVE
                     IEASFPGGSSAWRRYMERNLDASIPEKQKAPAGNYTVVIQFVVNLDGTVSDVRALTNH
                     GYGMETEAIRAIKKGPKWLPAIQNGRKIVAYRKQPITFVVDNGPKKESKLNAGQGTEL
                     NEVVVVGYSDPSIPTFPGGDSAWKKYLIVNANSMVSVNNGAPEGRYKTRIRFIVHPDG
                     TLSDITALTHFGYGMEEEAIRLIKNGPRWIPATRDGTKVSSYREQPITFELTSEPDKP
                     NYPPVIPSIKLTNITSPRLANGNLLKLDPGTIYIGLTNTILLETSGNDMKFITAETGN
                     GNMLKIENGKIEIQTNAVQDQFAIKLFYTKDNIKTTVKTVNFPVRLIPQQ"
     gene            complement(3107..4276)
                     /locus_tag="PLU11_05360"
     CDS             complement(3107..4276)
                     /locus_tag="PLU11_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPN58574.1"
                     /translation="MSLYSKRGVSAQKEEVHAATKNLDKGLYANAFCKIYPDVLCGDD
                     DWVNLMHADGAGTKSILAYLYWKETGDVSVWKGIAQDAIVMNLDDLLCVGIYDQLLFS
                     STIDRNKNVIPAEVLEAVINGTQAFFDTMKGFGVNITFMGGETADVGDVVRSIAVNGT
                     MAARWPKSKLITNEKIGPGDVIVGLAGFGKANYETEYNSGLASNGLTSARHDVLHKTY
                     GARFPESYDTSLDESVVYIGPHRMTDEVKDGDTTYEIGKLLLSPTRTFAPVMKALLEN
                     HFDAIHGLIHCSGGGQTKCMKYIPENVRIIKDNLFTPPIIFNIIQAASKSDDREMYQV
                     FNMGTRLEIYTNEKDAEAIISVSKSFGVDAKVIGRVEASEKKELHILTPNGQLVY"
     gene            4614..5543
                     /locus_tag="PLU11_05365"
     CDS             4614..5543
                     /locus_tag="PLU11_05365"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPN58575.1"
                     /translation="MQVHHDIEQLPVFRNAVITIGTFDGVHKGHRKILDKMKTEAEAN
                     GGETVIITFHPHPRKIVSSEFMGIRLITTMEERIGLLENLGIDHLVVVPFTDVFANQS
                     AEEYIRDFLVEKFHPHTIIIGYDHRFGRDRVGNFQLLEEKSPEYGYRLVEIPKHVLEN
                     ISISSTNIRKAIIHNDTDTVNKVLGYDFFFSGLVVHGNKLGRKLGYPTANLKVKDEDK
                     IIPGNGIYAVYAQPEGYSEPLKGMMSIGFRPTVDGKQRVIEVNIFDFDQEIYDQQLTV
                     FVKKYLRPELKFDGLEPLVKQIDQDKIDSLAVL"
     gene            complement(5610..6362)
                     /locus_tag="PLU11_05370"
     CDS             complement(5610..6362)
                     /locus_tag="PLU11_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58576.1"
                     /translation="MKKMFFSLLTACSLFLAGCLETTQEVSINEDGSGTASYTTDMSA
                     LIGMLKQMGSSEDLKEMNQVLDSTISMNTIADSIPDLTDAEKSLMKKGEMKINMDMKE
                     ERFLTKLNFPFGQVSDIASLDQLSGKVVGEALKGQGGMNGLTGEGGSPQMSSFEEYFK
                     TVYTNGRIQKTLIKEKYEQVGEDEFFKSMKEASAMGLAMKANYIINLPRPASKAEGKG
                     ITLSEDKKKITIAVDMDDFFDEPAKFEYLIEY"
     gene            complement(6452..7447)
                     /gene="holA"
                     /locus_tag="PLU11_05375"
     CDS             complement(6452..7447)
                     /gene="holA"
                     /locus_tag="PLU11_05375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPN58577.1"
                     /translation="MSVEKIISDWKKKQFRPIYWLEGDEEYFIDKAVDYAEHHILSES
                     EASFNLSIFYGKDANWADVLNACRRYPMFAERQVVLLKEAQQMKDVEKLEPYMENPLV
                     STILVVSYKDKKLDARKKFSKLVKENGVVISTKKLYDRDIPAWTQDMVQSKGLTITPK
                     GLALLSDHIGNDLSRIENEIEKLSVNLGKRTQINEDDIERYIGVSKEFNVFELQSALA
                     AKDLSKAIRIVQYFESNPKANPIQLILPSLYSFFSKVFMIQAISGDDRTIAGHIGVNP
                     FFVKDYIQAYRLYGSSGVENILMLLHHYNLKSIGVGSGSASDGSLMKEMVIKMMS"
     gene            7659..8627
                     /locus_tag="PLU11_05380"
     CDS             7659..8627
                     /locus_tag="PLU11_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58578.1"
                     /translation="MTNTNYPSATPQSQPNRNKNSKNIIIGLLAAGLLGTWGYLLWDK
                     NKSGEKILQQQTQISALDSSKADVQMEFDNALARLDSVTGANNNLQGQLSDKQKDIEA
                     KKSEIRRILNDKNASQAQLTKAKGMINELNDKITSLEAEVARLTGENQELTSANTTLT
                     TEKATLETNLTTTKTEKESLEKTVDVGSTFSASNIQVTPVDERKSGKEKSTSTAKKVD
                     KLVVSFNVENRIAKSGPADMYLIVTAPDGKVISEAGSVLNTRTDGDKTFTARIPVNYE
                     QGTRMPVSFPIKQNDFKTGNYKVEVYHNGFKIGEGVRSLKKGGIFG"
     gene            8797..9948
                     /locus_tag="PLU11_05385"
     CDS             8797..9948
                     /locus_tag="PLU11_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58579.1"
                     /translation="MLYTFRRKTALSFTLSFFSLVAVAQNAVQTKYTNSSVYTGIEVG
                     AKGVKMSIIEIGKNAQSTGAFNVLKDTSVNTDFISFSPSTFTATLEGFSALYTKATRE
                     LKIPGKKIFTVISSGVKMQAEKENKSAMIKELIDSFRVRIGEPERIVEVVDVIQEARL
                     SHLGIVPDKRRFTTFLIDIGSGNTKGGFFPDGRSTSNFKLFQLNWGTKSTFNEAEKRT
                     DGDMSLPNYNKHLVRVLQNAEEADIIYAVNASGAYPLSDNIAVSGGIAWATATLLHPE
                     LIDNPVISITFDELVKFNEKLAKNFSALSADFLVSNINNAEDKERAKTEILRVHKVFD
                     QKSLMAGAGLLWKIMRQFESAFETKHFYLVKNGQVGWVSAYVDQTISGN"
     gene            9999..10925
                     /locus_tag="PLU11_05390"
     CDS             9999..10925
                     /locus_tag="PLU11_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58580.1"
                     /translation="MKDIKTSLLVMLSVGLVGTWIYHLYDKTQYASQRKEIFIKDSIA
                     VAEAVQDSLQQLYARSMQTLDRKLDSTQFGADSLRFTLETKLVEINRLRNEINSILKN
                     RGASQSDLLTARQKISELQTLVDDMKGQKLSLEEEKLKLAETMTRLNGEINGLQDNVK
                     KLGDENKSLAEKMNLASLFVASEIKLSPVTVKNDKEVETNQAKKTSKLVISFTVQNNI
                     TDFDATDVFIIITQPDGKVLTPDVWESATPMTTHSGDRKSYTRKVRFEYSKGEAKQMT
                     FSINADAYQTGAYSLQLYHKGYKVGQTSKNLY"
BASE COUNT         3441 a         2635 c         2408 g         2934 t
ORIGIN      
        1 tgatctccaa agaagactat tcgggtaaga gtatcaaaca gctggcgaaa aactatttcg
       61 aaggttcgtt cagtaatgta gtgtcctatc tggtaaaaga aaacaatatt tcggtggatg
      121 aactggagct attgctcaaa caaattaaaa aacagcaaaa ccagtccaaa tgaatccatt
      181 gctcaactat gggatccagg tgatcattgt ctccggtctc ttatacagct attatcattt
      241 tgtactacgg aataaaaaat tccaccgtta taaccgattt tacctgctgg gagcggcagt
      301 gctgagcgtg ctgattccct tgctcaatat ccccgtctac ttcagtgaag cagataccga
      361 atcctctatt gtgtataaca ccctgcgtgt attcgccact acggaaacag atgaagcagt
      421 caccatctat gccggtatac cggaaaaaga aagctggttc accctgaccc atatctttca
      481 actggtatat ttcacaatcg ccatcgttgt attattaagg gtcatcttct cattgatgaa
      541 aatccgccgc atcatccggc aacatccggt ggaaaaaatt gaaaatatca gctttttgaa
      601 taccgatgaa cccggcaccc ctttctcttt cttccgctgg ttgttctgga accggaagat
      661 cgagctcaac tctgaaaaag gcgaacagat cttccggcac gaactctttc atatagaaca
      721 aaagcatagt ctcgatatta ttttcctgga actcttaacg gtcgcaggtt ggttcaatcc
      781 cttcttccat ctgatgaaaa gagaaaccaa ggccatccat gaattcctgg ccgatcagtt
      841 tgccgttacg gaaaacaacc gctgggacta tgccgagctc ctgctgatgc aggtgctgaa
      901 caccaaccaa cgactcgtcc atcctttttt ccataaccaa atcaaaagac gtatagccat
      961 gattacctct cctcaaaaaa caagtcatca gtacctgaga aaactgatgg tgctgccggt
     1021 aacagcagta gccgtaatat tgtttgcttt taactataaa aacagtaatt ataatctccc
     1081 ggaaaccgag tccggggaaa agcaaactat tattattgac gttagtcacg gaggaaatga
     1141 tcccggggct attggagctg atggaacgaa agaaaaagac ttagtacttg ctattgctca
     1201 aaaaatcagg tcactcaacc aaaacgggaa ttacaacatt gtattaaccc gggacggaga
     1261 ttttctccct gaattaaaat cacgggtaag tacaggtaat gaccaggccc cagccctatt
     1321 catttctcta catattaaca gttcaccaga caaacagaaa aatgggattg aagtctatat
     1381 acctaaaaac gaaaacaagc attttaagga aagcaaagcg tttggatcaa tcctgattgg
     1441 taagctatca gaaaattatg aagctggccc caatatttta caacgtagcc aaacgatctg
     1501 ggtattagag aacagtaatt gcccggctac actggtggaa atcggataca tcactaacga
     1561 aaaagactta tatcatatcc gaaatactaa taaccaggaa aacattgcaa aaaaaatatt
     1621 ggaggcaatc tatggatata atatccagga tcaggataaa aaagtaaact actcatcttc
     1681 aataacaaat gatactacca ttaaaccaac ccgaaaaatg gctacgtttg accaaatggt
     1741 catagtaagt gacagtatta ttctcaagag ctttccttcg aaagccctga aagataaaaa
     1801 acctgaagct attgtattca tgaatggcaa acggattccc aaaagtgaat tcgtcaacaa
     1861 aaccatcctt tgcgacagtt ttgaatatta cgatgcgggt gataaaaaag cgatcgaact
     1921 ttatgggccg gaggccaagg acggggtcat gatcattcac aatggtaaaa tcacggatac
     1981 tgaatttgaa ttaaaagaag caaataagcc tgaggaagat aaaatctttt ccaaagtaga
     2041 aatcgaagct tccttccccg gaggatcaag tgcctggcgc agatacatgg aaagaaacct
     2101 ggatgcaagt atcccggaaa aacaaaaggc acctgctggc aattataccg tggttattca
     2161 gtttgtggtc aacctggacg gtacagtaag cgatgtacgt gcactgacta accatggcta
     2221 cggcatggag acggaagcaa taagggcaat taaaaaaggg cccaaatggt taccagccat
     2281 tcaaaatggc cggaagatag tggcctaccg gaaacaacct atcacttttg tagtagataa
     2341 cggccccaaa aaagaaagca agttaaatgc cggacaaggt acagagctga atgaagtagt
     2401 agtcgttggc tactcagatc ccagcatccc aacttttcca ggtggtgatt cagcctggaa
     2461 aaaatatctt attgtcaatg ccaattccat ggtatctgta aataacggcg cccctgaagg
     2521 tagatacaaa acaagaatcc ggtttattgt acacccggat gggaccctct ccgatataac
     2581 tgcattgacc cattttggat atgggatgga agaagaagcg atccgattga taaagaatgg
     2641 tccccgttgg atacctgcca cccgggatgg cactaaggta agcagctata gagaacaacc
     2701 tattacattt gaactgacaa gtgagcccga caagcccaat tacccgccag tcattccctc
     2761 catcaaatta acaaacatca cttctccccg gcttgccaat ggaaaccttt tgaaattgga
     2821 tccgggtact atttacatcg gcctgaccaa tacaattttg ctggaaacat ccgggaatga
     2881 tatgaaattt attacagctg aaacaggaaa cgggaatatg ctgaaaattg aaaatgggaa
     2941 aatagaaatt cagaccaatg ctgttcagga tcaattcgcc attaaattgt tttataccaa
     3001 agacaatatt aaaacaacag ttaaaacggt aaatttcccg gtcagattga taccacagca
     3061 ataaccgcgg attatctgaa tgaaggtaat gaactaagca aaatctttaa tagacgagtt
     3121 gtccattggg agtaagaata tggagttcct ttttctcact tgcttccacc ctgccgatca
     3181 cctttgcatc cacgccaaaa cttttggaga cgctgataat cgcctccgcg tctttttcat
     3241 tggtgtatat ctccagccgg gtacccatat tgaaaacctg gtacatctcc cggtcatcgg
     3301 atttactggc cgcctggatg atgttgaaga taatgggagg tgtaaataaa ttatccttga
     3361 tgatccggac attctccggg atatacttca tgcacttggt ctgtccgcct ccactgcaat
     3421 ggatcagtcc gtgtatggcg tcaaaatgat tttccagaag ggctttcatg acaggtgcga
     3481 atgtccgggt gggtgataac aagagcttac ctatttcata ggtagtatcc ccgtccttca
     3541 cttcatcggt catgcggtgc ggaccgatat agaccacgga ttcatccagg gaggtatcat
     3601 aactctccgg gaaacgcgct ccataggttt tatgcagtac atcatgacgg gcactggtga
     3661 gaccgttact ggccaatcca ctattgtatt cggtttcgta attggctttc ccgaacccgg
     3721 ccagtcccac aatcacatcc ccgggcccga tcttttcatt cgtaatcagt ttgctctttg
     3781 gccagcgggc ggccatcgta ccattcactg caatacttct taccacatcg cctacatcgg
     3841 cggtttcccc gcccataaag gtgatgttca ccccaaatcc tttcatcgta tcaaagaaag
     3901 cctgggtgcc attgatcacg gcttccagca cttcagcggg gattacattt ttattgcggt
     3961 cgatcgtgga ggagaaaagc agctgatcat aaatccccac acagagcagg tcatccaggt
     4021 tcatcacaat cgcatcctgg gcaatgccct tccagaccga cacatccccg gtttctttcc
     4081 aatataaata ggcgagaata ctcttggtcc cggccccatc cgcatgcatc aggttcaccc
     4141 agtcatcgtc cccgcagaga acatccgggt atattttaca aaaagcgttc gcataaaggc
     4201 ccttgtccag gttcttcgta gcggcatgca cttcttcttt ctgggccgaa acgcctcttt
     4261 tcgagtataa cgacatcttg ggggtgtatt tgctgcaaac ataattaaag ttggggattg
     4321 ggggattggg aattggggat tgaggggtga ggtaatgaaa ataataataa gatgccttgt
     4381 catcccgacg aaggagggat tagtcgattt ttcctcccag aggtatttaa actccgacgt
     4441 aggaggagtc ttgtattacc cggactgttt gagattcctc gcttcgctcg gaattttcat
     4501 attgaatttt tgttactttt ttgtccctcc ttcgtcggga tgacaactat ttcccaggga
     4561 atttatcagc tctttactaa cgactaaaga ctatcgacta tcttcgcatt cctatgcagg
     4621 tacaccacga cattgaacaa ctaccggtct tccggaatgc cgtaatcacc attggtacat
     4681 tcgacggcgt acataagggc caccggaaga ttctggacaa gatgaaaacg gaagcagaag
     4741 ccaatggcgg agagaccgtc attattactt ttcacccgca tccccggaaa atagtttcct
     4801 ctgagtttat gggtatccgc ctcatcacaa ccatggagga gcggatcgga ttgctggaaa
     4861 acctggggat cgatcacctg gtggtggttc cttttacgga tgttttcgcc aaccagtcgg
     4921 ccgaagaata tatccgtgat tttctggtag aaaaattcca cccgcatacc attatcatcg
     4981 ggtatgatca ccgttttggc cgcgaccggg tgggtaattt ccagttactg gaagaaaaat
     5041 ctcctgaata tggttatcgc ttagtggaaa tccccaagca tgtgctggaa aacatttcca
     5101 tcagttccac caatatccgt aaagccatta tccataatga cacggacaca gtgaataaag
     5161 tactgggcta tgatttcttt ttctccggcc tggtggtaca cggaaataaa ctgggacgaa
     5221 aactgggcta tcccacggcg aatctgaaag tgaaagacga ggacaagatt atccctggca
     5281 atggtattta cgctgtctat gcacaaccgg aaggatattc agaaccctta aaaggaatga
     5341 tgagtatcgg ctttcgtcca acagtggatg gcaaacaacg ggtcatcgaa gtgaatattt
     5401 ttgattttga tcaggagatc tatgaccagc aactgaccgt ctttgtcaaa aaatacctcc
     5461 gccctgaatt aaaatttgac ggactggagc ccctggttaa acagatcgac caggataaga
     5521 tcgacagcct cgcagtacta tgataaaaaa ctattcaata aaaaagggct gaccaaccgg
     5581 tacagccctt tttaatgcag taaaaactat taatactcga tcaggtattc aaatttggcc
     5641 ggctcatcaa agaaatcatc catatccacg gcaatggtaa tcttcttttt atcttcagaa
     5701 agggtgatgc ctttgccttc cgctttgctg gccggacggg gcaggttgat gatataatta
     5761 gctttcatag cgagacccat agcggatgct tccttcatgg atttaaaaaa ttcatcctcg
     5821 cccacctgct cgtatttttc tttgatcagt gttttttgaa tccggccgtt tgtataaacc
     5881 gttttaaaat attcctcaaa agaggacatt tggggggatc cgccctctcc ggttaaaccg
     5941 ttcattccac cctgtccttt aagtgcctca cccactactt tcccggaaag ctgatccagg
     6001 ctggctatat ccgacacctg accaaaagga aaattcagtt tagtcagaaa tctctcttcc
     6061 ttcatatcca tgttaatctt catctcccct tttttcataa gcgacttctc cgcatccgta
     6121 aggtcaggta tgctgtcggc aatcgtgttc attgaaatcg tggaatcgag cacctggttc
     6181 atttctttca ggtcttcact gctgcccatc tgcttgagca tcccgatcaa tgcactcata
     6241 tcagtggtat aactggctgt gccgctgcca tcttcattaa tagaaacttc ctgggtggtt
     6301 tccaggcaac ctgcaagaaa caaggaacag gctgtcagga gggagaaaaa catttttttc
     6361 atgtacttag tattgatgat gtgttagatg tacgggagct gttttaagtt gcacaaagaa
     6421 agggaaaatc ggtgaacccg tgagaccgat ttcaggacat cattttaata accatttcct
     6481 tcatcaggga gccatcactg gcggaaccac tacctacccc gatgctcttt aaattgtaat
     6541 gatgcaggag cattaaaata ttttctaccc cgctgctgcc gtaaagccgg taagcctgga
     6601 tataatcttt tacaaaaaag ggattcaccc cgatatgacc cgcaatggtc cggtcatctc
     6661 ctgaaatcgc ctgaatcata aagaccttac tgaaaaagct atacagggag ggaagaataa
     6721 gctgaatcgg attggctttg ggattggatt caaaatattg tacgatccgg attgctttgg
     6781 acaggtcttt ggcagccagg gccgattgca gttcaaacac attaaattcc ttactaaccc
     6841 cgatatatcg ctcaatatcg tcttcattga tctgggttcg ctttcccagg ttcacggata
     6901 acttctcaat ttcattttca atccggctca ggtcattccc gatatggtcg gacagcaggg
     6961 ctaatccttt gggagtaatg gtcaacccct tggattgaac catatcctgg gtccaggcgg
     7021 gaatatccct gtcgtacagc tttttggtac tgatgaccac cccattctcc tttaccagtt
     7081 ttgagaattt tttccgggca tcaagctttt tatccttgta ggaaacaacc aggatggtgg
     7141 agaccagggg attttccata tagggctcca gcttttccac atctttcatc tgctgggctt
     7201 ctttcaggag caccacctgt ctttcggcaa acatgggata gcgacgacag gcattcaaca
     7261 catcggccca attggcgtct tttccataaa aaatacttaa attaaaggaa gcttccgatt
     7321 cggaaagaat gtggtgctcg gcataatcta ccgccttatc tataaaatat tcctcgtccc
     7381 cctccagcca atagatgggc cggaactgtt ttttcttcca gtcggagatg atcttttcca
     7441 cactcataag ccgctaagat acaggctttc acagaagcag gttgtcaaaa attatacacc
     7501 aaaaacccac tcattttcca ttctggcacg gttttcgaaa attataaaat gaatttaaca
     7561 ggttgtaagt aaacccaaaa aataacacgg taactttata tcctgattta tgtaaaaaca
     7621 tcaaataacc atcatttaag ctaaaaattc cattttatat gacaaacacg aattatcctt
     7681 cggccacccc acaaagccaa ccgaacagaa acaaaaatag taagaacatt attataggcc
     7741 tgctggcggc aggtttactt ggcacctggg gttatcttct ctgggacaaa aacaaatccg
     7801 gagaaaagat tttacagcaa caaactcaaa tttccgccct ggactcatcc aaagcggatg
     7861 tccagatgga atttgacaat gcattagccc gcctggattc cgtaaccgga gccaataaca
     7921 atctgcaggg gcagctttca gacaagcaaa aagacattga agcgaagaaa tcagagatcc
     7981 gccggattct gaatgacaaa aatgccagtc aggcacaatt gaccaaggcc aagggcatga
     8041 tcaatgaact gaatgacaaa atcaccagtc tggaagcaga ggtggcccgt ttaacaggtg
     8101 aaaatcagga acttacatct gcaaacacca cacttactac agaaaaagct accctggaaa
     8161 ccaacctgac cacaactaaa acagaaaaag aaagcctgga aaaaacagtg gatgtgggtt
     8221 caaccttcag cgcgtctaat atacaggtaa ccccggtaga tgagcgtaaa agtggtaaag
     8281 agaaaagcac ttccactgcc aaaaaagtgg ataaactggt ggtttccttt aacgtggaaa
     8341 acaggattgc caaatctggc ccagctgata tgtacctgat tgtaacggca cctgacggca
     8401 aagtgatttc tgaagccgga agtgtactga atacccgtac agacggagat aagaccttta
     8461 ccgcccgtat ccccgtaaac tacgaacaag gtacccggat gccggtttct ttcccgatca
     8521 aacaaaatga tttcaaaacc ggaaactaca aagtggaggt ttaccataac ggattcaaaa
     8581 ttggcgaagg cgtaagaagc ctgaaaaaag gtggtatatt cggttaaagc agttaactcc
     8641 tgctaaaaga accgtcccgt ttaccggggc ggtttttttt gcctgttatg agtcaactta
     8701 accacaactt ttacacattg aagaacggct gcaaaatcaa ctaagtaatt ccgcgtggta
     8761 ttttagtatt tttatcacct aagtaaaaca taaaccatgc tgtacacttt tcgccgcaaa
     8821 acggccctgt cgtttacact ctcttttttc tctttagtag ctgttgctca aaatgctgta
     8881 cagactaaat acactaactc ttccgtatac accggcattg aagtcggtgc caaaggcgtg
     8941 aagatgagta taattgaaat tggtaaaaat gcacaaagca caggtgcatt taatgtgctg
     9001 aaggatacat cagtgaatac cgattttatc agcttctctc catccacttt tacggcaaca
     9061 cttgaaggtt tttccgcctt gtatacaaaa gccacccgtg aattaaaaat tcccggcaaa
     9121 aaaatattca ccgtaatcag cagtggcgtt aagatgcagg cagaaaaaga aaacaaatca
     9181 gcgatgatca aagaactgat tgattctttc cgtgtacgta ttggtgaacc cgagcgaatc
     9241 gtcgaagtgg tggatgtgat acaggaagcc cggctttccc accttggaat tgttcctgac
     9301 aaacgccgct ttaccacttt cctgattgat atcggaagcg gaaatacaaa agggggattc
     9361 tttcctgacg gccgcagcac cagtaatttc aaattgtttc agctgaactg gggaaccaaa
     9421 agcacattta acgaagccga gaaacgcacg gatggtgaca tgagcctgcc caactacaat
     9481 aagcatctcg ttcgtgtttt acagaacgcg gaagaagcgg atatcatcta tgcggtgaat
     9541 gccagcggcg cctacccgct cagtgataat atcgcggtga gtggtggtat tgcctgggcc
     9601 actgccaccc tgctgcaccc cgaactgatc gataacccgg ttatttccat tacgtttgat
     9661 gaactggtga aatttaatga gaaactggcg aagaacttct ccgccctttc agctgatttc
     9721 ctggtttcaa atatcaataa tgccgaggat aaagaaagag ccaaaacaga aatcctgcgg
     9781 gtacacaaag tgtttgatca gaaatccctg atggctggcg ccggtttact ctggaaaatc
     9841 atgcgtcagt ttgagtctgc ctttgaaacc aaacatttct acctggtgaa aaacggtcag
     9901 gtgggctggg tttctgctta tgtggatcag acgatttccg gtaactgatt acctttctat
     9961 catccaaaac aaaatctgta caacacccaa tcgtgccgat gaaggatatt aaaacttcgc
    10021 tgcttgtgat gctttctgtg ggactggtag gcacctggat ctatcacctc tacgacaaaa
    10081 cgcagtacgc ttcccagcgt aaagaaatct ttataaagga ttctatcgcc gtggccgaag
    10141 ctgtacagga ttccctgcag cagctctatg cccgttccat gcaaaccctt gaccggaaac
    10201 tggactccac tcaattcggg gccgattcgc tccggttcac cctggagacc aaactggttg
    10261 aaatcaaccg cctccggaat gagattaatt ctattctgaa aaacaggggt gccagtcagt
    10321 ctgacctgtt gacagcccgc cagaaaatta gcgaactcca gaccctggtg gatgatatga
    10381 aaggacagaa actcagttta gaagaagaaa agctgaaact ggcggagaca atgacccggt
    10441 tgaatggaga gatcaacggt ctccaggaca atgtgaaaaa actgggcgat gaaaacaaat
    10501 ccttagcgga aaaaatgaac ctggcctctc tttttgtagc atcagaaatt aaactcagcc
    10561 cggttacggt gaagaacgat aaagaagtgg agaccaacca ggcgaaaaaa accagtaaac
    10621 tggtcatctc ctttaccgta cagaataata tcacagactt cgatgccacg gatgttttta
    10681 tcatcattac ccaaccagat gggaaagtcc tgacaccgga tgtgtgggaa tccgccactc
    10741 ccatgaccac ccatagcggc gaccggaaaa gttatacccg gaaagtccgc tttgaataca
    10801 gcaaaggcga agccaaacaa atgaccttct ctataaatgc agatgcctac cagacaggcg
    10861 cctattcgct tcagctttac cataaaggat ataaggtagg acagacgagt aagaatcttt
    10921 actagtacta ctacggcagc tggttataaa aagtaagttt ttcattctta ggaattattg
    10981 ggagaatgtg ccaattagcc aatgtgccaa tgtgccaatt gacctcctga gagaaattac
    11041 aaaaaattca caatacaccc ctaaggggtg aaatgaaaac ctcattctct tgtatttttt
    11101 gctaccaaca atgctcccct acggggtgag aagatttaat ctcagggctg caagcttcat
    11161 cagtcagtag tttaattggc taattggcat attggcacat ttgtattatc tctactacat
    11221 atacttaaag cctattgtct tctgaaaccc ctcctaaaaa aaaagtcccg ccgaagcggg
    11281 acctgtatac ttataaccca ataacattca attatcaaaa caggttgtag ccatagctca
    11341 ggtagtaaag accacctact gaggggctgc cgaatccggt tctggcgtag aagttaccga
    11401 tgttggtacc accggcac
//
LOCUS       DAOPMW010000069        11398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6037_length_11398_cov_6.273561, whole genome shotgun sequence.
ACCESSION   DAOPMW010000069 DAOPMW010000000
VERSION     DAOPMW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_11398_cov_6.273561"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4411
                     /locus_tag="PLU11_05395"
     CDS             <1..4411
                     /locus_tag="PLU11_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58581.1"
                     /translation="VFRWTISYSNCPPSTDDVQIIIYDNPSTAEAGPNQEICGTGTQL
                     AATAATTGTGLWTFVSGPNTPLIANPAQPNSLLSNLIPGIYQLRWRITNGVCPVSDDL
                     VQITVGALPTPSNAGPDQTFCAATTITLQGNTPTTGTGTWTQLAGAPAIITNPALPTT
                     TVTGLLPGNYTFEWTITNSVCPPSQDQVIITNYNTIFNQINTTPQTLCAGQTLQINGQ
                     VPTGGNGIYSFQWQQSVDGLTWTDIPGATGQNLTYTVNSSLFIRRVVTSLPCTSNSQT
                     VFITVLAAITNNTLNGNQSVCINTAPAIIIGSVPSGGDGTFVYSWEISTDGGATWTLI
                     TGAATKDYAPGVLIQSTCYRRLVSTNLCSGPQSNTSPSICVTVNPDAQALFTYPADTA
                     CAAFAIQIQNTSPANRHLRYDWYADNNFIASGQAFPGFTIPGPGQSVTIKLVAISAFG
                     CKNDSLSHLFATKPTANPSFTASDTVGCGPLTVSFTNTTSQLTAFSYFWDFGNGVTST
                     LAQPGPVVFNPNPNFRDTIYIVRLFAFTECDTQVFIVPVRVMSKPRAVFTPDRTTGCS
                     PVTVTFNNLSQGIGMSYFWDFGDGATLSTSVYGPVQHTYITGIQRTYPVRLRVTNNCG
                     VDSFLVNIVVTPNTIQLDFAVDGTTVNGCIPHTVRIVNNSAGGNLFQWDFGDGNTYNS
                     GSNVEVINHTYFQTGSFTISVRAINSCSDTTGTETITVYRKPIPAFTYTTNTSCIGDN
                     VQFNNLTDTATLYTWNFGDGNFSNLRHPAHFYTNPGVYTITLYSSLQHATGIVCTDST
                     KQLITITDRLPGLFLVSDTLGKCLPFTVTFSNQSTAALTAVWDFGDGNTGIGNTVSHT
                     YTANGNYLATMTATAPGGCIYNYSKPIVISGPTGTLNYTGGIVCGNAPVRFESIALNT
                     DSVRYDFGDGQFYTGPAGVIFHTYNQPGIFIPRLQLISGTNGTCRITLPAGDTIRVDI
                     LDAGFTQAAIRFCDSTVVNFSDTSRAYSGLSTYQWFFGDNQSAGLQNPRHAYTSNGNY
                     QITQIITSVWGCTDTARLLLPIQVNNTPVAQVNVAPTGCMKDTVTFSSNISSTDPVVL
                     TKWVLSNGVTNLNPSFSTVFSQTGTYTVTFIAGTANGCYDTTTTSITIHAIPAITASS
                     DQRICLGQTVQLNASGATNYQWSPLNSLSCYTCPNPIASPSITTSFVVSTISPFGCKN
                     TDTVLVQVVQPFDLTVSPNDTICIGQSTTLTVSGAQVYNWQPSQGLSCNNCLNPVASP
                     LQTTLYRVVGYDLYNCFTDTGYVTVAVGQYPVVSLGPDQVLATGTQLQMNNTVTNGPV
                     RTWTWTPSADLSCNNCPIPVANIRNDVCYSLTVRNIYGCAGSDTMCVKAFCNDTQVFI
                     PNAFTPDDDGKNDILMVRASGIQLVKKFIIFNRWGEIVFERSNVPPNNPQHGWNGTIK
                     GVKASSDVYVYIAEVICENGTSYSYKGNVTLLK"
     gene            4418..5437
                     /locus_tag="PLU11_05400"
     CDS             4418..5437
                     /locus_tag="PLU11_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPN58582.1"
                     /translation="MRAKLFTFLCLAVLSGIKLNGQDFTFSQFQEMPLLRNPAIAGLF
                     AGDIRIQSAYRSQWLSVTTPYKTMAISAETKIPAKYQDDFFTASLLITNDVAGDSRLS
                     RLQILPSINFLKSINDNNGYIALGFIGGLVQSQFDPTKLTFDDQFQNGSFNPLNPTSQ
                     TFSKTSITYFDASVGMSYSDRIGENTKFYLGGAYFHANKPKVSFLDDKVLLKPRSVIN
                     AGLSIETSEYDNFYIFGDYISQAGNRQGLMGLMYSHIIGEYMEDDNVSLSLGAFYRWG
                     DAVIPAIKLNLYKVSLGISYDMNVSKLSRASQLRGGYEFTASYRSFLNIRNTSLNKVK
                     CPVTF"
     gene            5509..6366
                     /locus_tag="PLU11_05405"
     CDS             5509..6366
                     /locus_tag="PLU11_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPN58583.1"
                     /translation="MRYNKNTHSPISQSPNPLFTLIFALLFFQTAHSASVDTVSIYSA
                     AMKKEYKCVVITPGMYKKATAVPVVYLLHGHAGWYANWILRVPELKTWADEYQLMIVC
                     PDGGFNSWYVDSPVDSTVRFETYISKEVPDYIDAHYKTIKSRHGRAITGLSMGGHGGL
                     FHGFRHADIFGACGSMSGGVDLQPFKKNWQISQKIGDTVNYAANWINYSVMTLVEQKP
                     KDSLAIIIDCGTEDFFYEVNGQLHRKMVGLKIAHDYIERPGKHDWNYWRNAVQYQLLY
                     FRKYFNNHG"
     gene            6409..7290
                     /locus_tag="PLU11_05410"
     CDS             6409..7290
                     /locus_tag="PLU11_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58584.1"
                     /translation="MFPATKSLIPAIAIVMVLYSCQTKNTNEEKINLQLIKSFNESNA
                     TIKRSTSLLLNALEDMTYDPVKTDRATPWFNKAVRINEFTRSLYSFIDTTIKKNTTEK
                     GIEAVYKKIITFKSDVLQTDVEMAEPFVTNFNFINPFFYLCGWDTILNKPLKNHVISK
                     VEIVLLLESLKNKAMNLENNLVSFCYQKTPTYDDGYDVYSMIAGQNANTFSPGSVLEL
                     KAGIGAFSKAAKPIITFGGDTVSINEEGYAVYRRKVPATPGHYKIPIRVRFYNLLFGK
                     EEIKEVIAEYKVVKACD"
     gene            complement(7779..8483)
                     /locus_tag="PLU11_05415"
     CDS             complement(7779..8483)
                     /locus_tag="PLU11_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58585.1"
                     /translation="MRFIFLLLLFISTSGMAQWKSFTLNKRGDTLNRVDLKGYKQGPW
                     IVSIPDLRGERGYEEEGYFENDQKEGTWRKFSLEGVKIAEENYRWGKLNGRQQYFTYN
                     GGLQRVENWRAMDPKVAFDTVAVYDIKDPSKIIDWVVVKNEGVAMRHGKWIYYDPREG
                     TVESTENWVMNKIQTEAAASGEDGEIGILTTSDGKTKSDTVGKKGPAKPQAILDYEKK
                     NSGKKKVKVRDGNTGN"
     gene            8625..11273
                     /locus_tag="PLU11_05420"
     CDS             8625..11273
                     /locus_tag="PLU11_05420"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58586.1"
                     /translation="MLLKKTAFSRMTAGLAAILLANSLLLTVAVAQPAPTPAAERLKV
                     TEQRKALKSRSLLNEIPFRNIGPSIMSGRVVDIDANPADPTEFYVAYATGGLWHTKNN
                     GQSFTPIMDNLDILFIGDIAVNWKTRAIWVGTGEVNSSRSSYAGVGIYKTNDNGKSWE
                     YLGLPESHHIGKIQLHPADNNTAWVAVLGHLYSANKERGVYKTTDGGKTWKQTLFVDE
                     NTGAVDMEINPSNPSELYAAMWYRVRRAWKFEESGKTSGIYKSTDGGETWKLVSAAGS
                     GFMTGDKIGRIGITVYPGNPKLVYAVVDNNTPKADTRKTNDSAYKKEDLKNLSKEQFE
                     QLSSKWLDTFLRANRFPRKYNAKGIKEMVAADKLKPTALWDYLDSDDGFQNTGIAGCE
                     VYKSEDGGQTWKKANEKPISIFNTYGYYFAKIYTSHTNPDKVFILGFFAQYSTDGGKT
                     FKTMDKNNVHADHHALWVNPKNDSHVINGNDGGTNISYDNGDHWFKANTPSVGQYYAI
                     TVDDAKPYNVYGGLQDNGSWWGPSNNRESIDWTDNGQYAYRMINGGDGMYAQVDTRDN
                     STVYSGSQFGFYGRYNKTIRGSQKSLHPQHELGDKPLRFNWLTPIQLSKHNQDVLYYG
                     SNRFHRSLNKGDSLAVLSADQTNGRVSGNVPYGTMTTISESPLRFGLIYTGTDDGNIH
                     VSKDGGYSWEQLNQVNTDEVKSKKSKVKIADSKLNTQNLWVSRVTASQYKESRVYTSL
                     NGYRFDHFAPYLYVSDDYGTNWKQIGKDLPNEPINVVKEDPKNDSILYVGTDGGLYVS
                     FDAGNSFMLWNNGMPESVPVHDIAIQARENEIVLGTHGRSLYVASLNDVHAVKKDPEW
                     MKKKPKPKKEERRNEEEEDESKPEMD"
BASE COUNT         3116 a         2840 c         2512 g         2930 t
ORIGIN      
        1 tgtattcagg tggaccatca gttacagcaa ttgtccgccc agtacggatg atgtacaaat
       61 aatcatatac gacaatccca gtacagccga agccggaccc aaccaggaga tatgcggtac
      121 aggcacacaa ctggccgcca cagctgccac taccggtaca ggcttatgga cctttgtcag
      181 cggtcccaac acacctctca tcgcaaaccc ggcccagccc aatagtctac ttagtaatct
      241 gatcccgggt atttatcaac tgagatggag aattaccaat ggcgtttgtc cggttagtga
      301 tgacctcgtt cagataaccg taggtgccct cccaacaccg tcgaacgcag gaccggatca
      361 gacattctgt gcggcaacga ccataaccct gcaaggcaat acgcccacaa cgggaactgg
      421 cacctggacc cagttggcag gtgcaccagc cattatcact aacccggcat tacctacgac
      481 aacagtcacc ggtttactgc ccggcaatta tacttttgag tggacaatca ccaacagtgt
      541 atgtccgccc agccaggacc aggtgattat tactaattac aatacgatct ttaatcagat
      601 caataccacc ccgcaaacac tttgtgcagg acaaaccctt cagatcaatg gccaggtgcc
      661 cacaggagga aatggcatct acagtttcca atggcagcaa agtgtggatg gtcttacctg
      721 gaccgatatt ccgggtgcca ccgggcaaaa cctgacctat acggttaaca gttccctgtt
      781 tatccggaga gtggttactt ccctgccctg tacttcaaac agtcaaaccg ttttcattac
      841 ggtactggct gcgattacca acaatacgct caacggtaac caatccgttt gtatcaatac
      901 agcacctgcc atcatcatcg gttcagtacc ttcaggaggg gatggtacat ttgtttacag
      961 ttgggaaata agtacggatg gcggagcgac ctggacattg atcacagggg cggctaccaa
     1021 agattatgcg ccgggtgtgt tgattcaatc cacctgttat cgccggctgg tatccaccaa
     1081 tctttgttcc ggtccacaaa gcaatacctc gccatcgatc tgtgtcacag ttaacccgga
     1141 tgcacaagcg ctttttacct acccggccga tacggcctgt gctgcctttg cgatacaaat
     1201 acagaacact tcccctgcca atcgtcatct ccggtatgac tggtatgcgg ataataactt
     1261 tattgcctcg ggacaggcct ttcctggctt tacgataccg ggacccgggc aatccgttac
     1321 gatcaagctg gtcgcgatca gtgcctttgg ctgtaaaaat gatagtctct cgcatttgtt
     1381 tgcgaccaaa cccaccgcca acccctcctt caccgcaagt gatacagttg gctgcggtcc
     1441 gctgacggtc agcttcacca atacgacgtc acaactgact gctttctcct atttctggga
     1501 tttcgggaac ggagttactt ccaccctggc tcagcccgga ccggtggttt tcaatccaaa
     1561 tccgaatttc agggatacca tctatattgt ccgcctgttt gcctttactg aatgtgatac
     1621 ccaggtattt attgttccgg ttcgggtgat gtcaaaaccc cgggccgtat ttactcctga
     1681 ccgtaccacc ggttgttctc ctgttacagt cacattcaat aacctgtcac aaggtattgg
     1741 catgagctat ttctgggatt tcggagacgg agctacttta tcgacttcgg tgtatggacc
     1801 ggttcagcac acctatataa ccggtattca acgaacctac cccgtccggc tcagggtcac
     1861 gaataattgc ggggtcgact ccttcctcgt gaatattgta gtaaccccca ataccattca
     1921 gctcgacttt gccgtggatg gaactacagt taatggctgt atcccgcata cggtacgaat
     1981 agtgaataac tctgcgggcg gcaatctgtt tcaatgggat ttcggcgatg ggaacaccta
     2041 taactcaggg agcaatgtgg aagtcattaa ccacacttat ttccagacag gttcctttac
     2101 catttctgtg agggccatca attcctgctc agacaccacg ggcacggaaa ccattaccgt
     2161 ctaccgcaaa ccgataccgg ctttcactta taccaccaat acctcctgta ttggcgacaa
     2221 tgtgcaattc aacaatctga cggataccgc cactttatat acctggaatt tcggggatgg
     2281 caatttctcc aatctccggc acccggcaca tttctatacc aatcccggcg tatacaccat
     2341 tacgctatac agcagcctgc aacatgccac gggcatcgta tgtacggata gtacaaaaca
     2401 actcatcacg atcacggatc ggttgcccgg cctcttcctg gtatctgata cgctcgggaa
     2461 atgtcttccg ttcactgtta cattcagcaa tcaaagtacc gcggcactta cggccgtctg
     2521 ggatttcgga gatggcaata ccggtatcgg taatactgtt tcacacacgt atacggctaa
     2581 tggcaattat ctggccacca tgacggctac cgctcccggg ggttgtatct ataattacag
     2641 taaaccaatc gtgatcagcg ggcctaccgg tacactgaac tataccggag gaattgtttg
     2701 tgggaatgcc ccggtccgct ttgaatccat cgcattgaat acggattccg tgcgctatga
     2761 ttttggagat ggccaattct ataccggtcc cgccggtgta atctttcaca cctataacca
     2821 acccggtatc tttattccaa gacttcaact gatttccggc accaatggaa cttgtcggat
     2881 taccttacct gcaggagaca cgatacgtgt ggatatcctg gatgccggct ttacccaggc
     2941 ggcaatccgg ttttgtgatt ctactgtagt gaatttcagt gataccagcc gtgcctattc
     3001 cggccttagt acctatcagt ggttttttgg cgataaccaa tctgcaggac tgcaaaatcc
     3061 ccggcatgca tataccagca atggcaacta ccagattact cagatcatca cttctgtatg
     3121 gggctgcacg gatacggcaa gattactctt acccatacag gtaaacaaca cgccggtagc
     3181 acaggtaaat gttgcaccaa cgggttgtat gaaagacacc gtgactttca gttcgaatat
     3241 cagttccacc gatccggtgg tattgaccaa atgggtactc tcaaacgggg tgaccaacct
     3301 gaatccttca ttcagtacag tattcagtca gacaggaaca tatacggtta cttttatagc
     3361 aggtacggca aatggttgct atgacaccac caccacttca ataacgattc atgccattcc
     3421 ggccattacg gccagcagtg atcagcggat ctgccttgga caaaccgtac agctcaatgc
     3481 cagtggcgcc acgaattacc aatggtcacc gctgaacagc ctgagttgtt atacttgccc
     3541 taaccctatt gcgagtcctt cgatcactac ctcatttgta gtatcaacga tcagtccgtt
     3601 tggttgtaag aatactgata cggtgctggt acaggtagtg cagccatttg acctgaccgt
     3661 ttcacccaat gatacgatct gtatcggaca aagcaccacg cttacagtaa gtggtgcaca
     3721 ggtttataac tggcaaccct cgcagggatt gtcctgtaat aactgtctga atccggtcgc
     3781 ctctccgtta caaacaaccc tgtatcgtgt ggtgggttat gatttgtaca attgttttac
     3841 cgacacaggt tatgtgacgg tggctgtcgg acaatacccc gtcgtatcgc tcggtcctga
     3901 ccaggtactc gccaccggta cgcagttgca aatgaataat acggtaacaa acgggccggt
     3961 tcgcacctgg acctggactc cgtcagcaga cctgtcttgt aacaactgcc caatccccgt
     4021 agccaatatc cgaaatgatg tttgctacag tcttacggtc cgtaacatct acggttgtgc
     4081 cggcagtgat accatgtgtg taaaagcatt ctgtaacgat acccaggtct ttatacccaa
     4141 tgcctttacc ccggatgatg atggcaagaa tgatatcctg atggtaagag caagtggtat
     4201 tcagctggtg aaaaagttca tcatcttcaa ccgctggggc gagatcgtgt ttgaacgaag
     4261 taatgtacca cccaacaatc cgcaacatgg atggaatggt accatcaaag gagtaaaagc
     4321 cagcagtgat gtgtatgtat atattgccga agtgatctgt gaaaacggaa cttcttacag
     4381 ctataaagga aatgtgacac tattaaagta attgcttatg cgtgcgaaat tattcacctt
     4441 cttatgcctg gctgtattgt cagggataaa actgaacggg caggacttca ccttctccca
     4501 gttccaggaa atgcccctgt tgcgcaatcc ggccatcgcc ggtttgtttg ccggggatat
     4561 tcggatccaa agtgcctacc ggtcgcagtg gctgtcggtt accaccccct acaaaaccat
     4621 ggccatcagt gccgagacca agatcccggc taagtaccag gatgatttct tcacagcttc
     4681 cctcctgatc accaatgatg tggctgggga ttcaagattg agccggttac agatattacc
     4741 ttctatcaat ttcctgaaat cgatcaatga taataacggg tatatcgccc tcgggtttat
     4801 cggcggactg gtgcaatccc agtttgatcc caccaagctc acttttgatg atcagtttca
     4861 gaatggttcc tttaatccgc tgaatccaac cagccagact ttctctaaaa cctccatcac
     4921 ttattttgat gcctccgtgg gtatgtcata cagtgaccgg atcggagaga acaccaagtt
     4981 ctatttaggc ggggcttatt tccatgccaa caaaccaaaa gtgtctttct tagatgataa
     5041 ggttttgctg aaaccgcgca gtgtgatcaa tgccggactg agtattgaaa ccagcgagta
     5101 tgataatttt tacattttcg gcgattatat cagtcaggcc ggtaaccgcc agggactgat
     5161 gggactgatg tacagtcata tcatcggcga gtatatggaa gatgataatg tcagtctttc
     5221 cctgggcgct ttttaccgct ggggcgatgc cgtaatccct gccatcaaac tgaatctgta
     5281 caaggtgtcc ctgggtatca gttatgacat gaatgtatcc aaactatcaa gggcctccca
     5341 actccgcggc ggctatgagt tcacggcttc ttaccggtcg ttcctgaaca tccggaatac
     5401 cagcctgaac aaggtaaaat gcccggttac attttaaaac tccatcaaat attgattatt
     5461 taatttaata accacattat ctttaatgtg gttattaaat taaaaagtat gcgctacaat
     5521 aagaacaccc attcccccat ttcccaatcc cccaatcccc tatttaccct gatattcgcc
     5581 ctgttgtttt tccagactgc ccattccgcc agtgtggata cggtgtctat atacagtgcc
     5641 gctatgaaaa aggaatataa atgtgtggtg attactcctg gtatgtataa aaaggcaact
     5701 gcagtacccg ttgtgtatct tcttcacggc catgccggct ggtatgccaa ctggatcctc
     5761 cgggtacccg aactcaaaac ctgggccgat gagtatcaac tgatgattgt ttgtccggat
     5821 ggcggcttca acagctggta tgtggacagc ccggtcgact ccacggtccg gtttgaaact
     5881 tatatttcca aagaagtacc tgattatatc gacgcccatt acaaaacaat caaaagccgc
     5941 catggccgcg ccatcaccgg actcagcatg ggcggacatg gtggcttatt ccacggcttc
     6001 cgtcatgccg atatatttgg tgcctgcggc agcatgagtg gcggggtgga cctgcagccc
     6061 tttaaaaaga actggcagat ctcccagaag atcggcgaca cggtcaacta tgccgccaac
     6121 tggatcaact actctgttat gaccctggtc gaacaaaagc ccaaagactc cctcgccatc
     6181 attatcgact gcggcacgga ggatttcttc tacgaagtca acggtcagct ccaccgcaaa
     6241 atggtcggcc tgaaaatcgc ccacgactat atcgaacgtc ccggcaaaca cgactggaat
     6301 tactggcgca atgcggtgca gtatcagttg ttgtatttca ggaagtattt taacaatcac
     6361 ggataaaata tttatcagcc acctgcgttt tgggtaaaat cccccaaaat gtttcccgct
     6421 accaaatcac tgataccggc aattgccata gttatggtat tgtattcctg ccagactaaa
     6481 aacacaaatg aagagaagat caatctgcag ctgattaaat cgttcaacga atcaaatgca
     6541 actattaagc ggtccacctc cttgctgtta aatgcattgg aagacatgac ttatgaccct
     6601 gtaaaaactg atagagccac cccctggttt aacaaagcag tcaggatcaa cgaattcacg
     6661 agatcactct atagtttcat tgacaccaca atcaaaaaaa atacgacaga aaaaggaata
     6721 gaagcagttt ataaaaaaat cataaccttc aaatccgacg tacttcagac agacgttgaa
     6781 atggcagagc cttttgtcac taactttaat ttcatcaacc ctttctttta tctgtgtgga
     6841 tgggatacca ttttgaacaa accattgaag aatcacgtca tttcgaaagt agaaattgtt
     6901 ttattgctgg aatccctaaa aaataaggca atgaacctcg aaaacaattt ggtcagtttc
     6961 tgctaccaga aaaccccaac ttatgatgat ggctatgatg tatatagtat gatcgccggt
     7021 caaaatgcga acactttttc gccaggctct gtacttgagc tcaaagcagg gatcggtgca
     7081 ttcagtaaag cagcaaaacc aattattact tttggaggag atactgtttc gattaatgaa
     7141 gaagggtatg cagtttacag gagaaaagta cccgctaccc cgggacatta caaaataccc
     7201 atccgcgtcc gcttctataa tctgcttttc ggaaaagaag aaatcaaaga agtgattgct
     7261 gagtataagg tggtgaaagc ttgtgactaa tattccgatg gtcattttag ttgaacccct
     7321 tcggggttcc aagggtcatc ggggacactg aaccccgggt ttcacccggg gctattataa
     7381 ttcaaccctt tcagggttgt aagaaaaatg catatcaagt gaggatattt atacagacaa
     7441 atgattcacc atcatttaac ttcccggttc tacattaaaa ggccgcccct acagggttta
     7501 agaggctatc gcatattatg ctaattacag gccaccccgc cggggttccg tttatactgt
     7561 tgttacacca gaaacttgtt ttctccctcg attattctat gaatgagcag cttcagcctt
     7621 cgataaagtt gttacggatt aataattcag gctgctgtgt tttttataga ctgttcagct
     7681 attatagttt ttaccattat tctgtatccg gaaatcaaca aatcctccag catccaacaa
     7741 acacttcaga ctcccgacta ctaactcctg actactaact agtttccggt gttcccgtcc
     7801 ctcactttca ccttcttctt ccccgaattt ttcttttcat agtcaagaat agcctgtggt
     7861 ttggccggtc ctttttttcc aaccgtatcg gatttggtct tgccatcgct tgtagtcaga
     7921 attccgattt caccgtcctc tcctgaagca gctgcctcgg tttgtatttt attcatcacc
     7981 cagttttcgg tggattccac cgttccttcc cggggatcat agtagatcca tttaccatgt
     8041 ctcatggcca cgccttcatt cttcaccacc acccaatcga tgatcttgga gggatctttt
     8101 atatcataga ccgccactgt atcgaaggcc actttgggat ccatcgcccg ccagttttcg
     8161 acccgttgca atccgccatt ataggtaaaa tactgctgcc gcccattcag tttgccccag
     8221 cggtagtttt cttcagcaat ctttactccc tccagggaga atttcctcca ggtaccctcc
     8281 ttctggtcat tttcaaaata accctcctcc tcataacctc tttcgcctct caaatcaggt
     8341 atactgacta tccagggccc ctgcttataa cccttcaaat ccacccggtt gagggtatca
     8401 cctcttttgt tgagggtaaa actcttccac tgggccatgc cgcttgtgga aataaagaga
     8461 agaagaagaa atataaatcg catagtttag taagtttgaa aaaagacaaa atacaaagta
     8521 cgaggtacga agtacgaagt acggaataca aagtacgaat cccgaagctt cgggacgagg
     8581 tacgaagtgc aaattactaa cgaaaattaa ctgcttttat gagtatgttg ttaaaaaaaa
     8641 ccgctttctc ccgcatgacg gcaggtcttg cggccatttt gctggctaac agcctgttgc
     8701 tgaccgtagc cgtggctcaa cccgccccca ctcccgctgc ggagcgtctg aaagtcaccg
     8761 agcagcgtaa agccttgaaa tcacgctccc tgctgaatga gattcctttc cggaatattg
     8821 gtccaagtat catgagtggc cgggtagtgg acatcgatgc caatcccgcc gatcccaccg
     8881 aattctatgt ggcttatgca accggaggac tctggcatac caaaaacaac ggacagagtt
     8941 tcacccccat catggacaac ctcgatatac tgtttattgg tgatatcgcg gtgaactgga
     9001 aaaccagggc catctgggtg ggaaccggtg aggtgaacag cagtcgttct tcttatgccg
     9061 gggtgggtat ttacaaaaca aatgataacg ggaaaagctg ggagtatctc ggtttgccgg
     9121 aatcccatca tataggtaaa atccagttgc atcctgctga taataatacc gcctgggtag
     9181 cggttcttgg tcacctgtat tctgccaata aagaacgcgg ggtatataaa accacagatg
     9241 gtgggaaaac ctggaaacaa acgctgtttg tagatgaaaa taccggggcg gtggatatgg
     9301 aaatcaatcc gtccaacccc tctgaacttt atgcagcgat gtggtaccgg gtacgccggg
     9361 cctggaaatt tgaagaaagc ggcaaaacca gtggcattta taaaagcacc gatggcggcg
     9421 aaacctggaa acttgtttct gcagctggtt ccggcttcat gaccggcgat aagatcgggc
     9481 gtatcggcat aacagtttat ccaggcaatc caaaactcgt gtatgcagtt gtggataata
     9541 acacgcctaa agccgacacg cgtaaaacaa atgattctgc ctacaagaaa gaagatctga
     9601 aaaacctcag caaagaacaa tttgaacaac tcagcagcaa atggctggac acgtttttaa
     9661 gagctaaccg ttttccccgc aagtacaatg caaaaggaat taaggaaatg gtggccgctg
     9721 ataaattaaa acccactgcc ctctgggatt atctcgacag tgacgatggt tttcagaaca
     9781 ccggcatcgc cggttgtgaa gtgtataaaa gtgaagacgg tggacagacc tggaaaaaag
     9841 ccaatgaaaa accgatcagt atattcaata cctacggtta ttattttgcg aagatctata
     9901 cctcgcatac caaccccgat aaagttttca ttctcggttt cttcgcccag tattccaccg
     9961 atggcggaaa gacgtttaaa accatggata agaataatgt ccatgccgat caccatgccc
    10021 tttgggtaaa cccgaagaat gattcccatg taattaatgg taatgatggc ggtaccaata
    10081 tcagctatga taacggggac cattggttca aagccaacac gccttccgta ggccaatatt
    10141 acgcgatcac cgtggatgat gccaagccgt acaacgtata tggcggtctt caggacaatg
    10201 gcagctggtg ggggccttcc aataaccggg aaagtattga ctggacagat aatggacagt
    10261 atgcttatcg tatgattaat ggcggtgatg gtatgtatgc ccaggtggat acccgggaca
    10321 attccaccgt gtattccggt tcccagtttg gtttttatgg ccgttataac aagactatcc
    10381 gtggttccca gaaatccttg catccccagc atgaactcgg cgataaaccc cttcgcttca
    10441 actggctgac cccgatccag ctgagcaaac acaaccagga cgttttatac tatggcagca
    10501 accgtttcca ccggtccctg aacaaaggcg attcactggc ggttctgagt gccgaccaaa
    10561 ccaatggacg ggtttccggc aatgttcctt atggaacgat gaccaccatc tccgaatcac
    10621 ctttacgttt cggcctgatc tacaccggca cggatgatgg gaatatccat gtcagtaaag
    10681 atggaggata tagttgggaa cagctgaacc aggtaaatac agatgaagtc aaaagtaaaa
    10741 agtcaaaagt aaaaatagca gactcaaaat tgaatacaca aaacttatgg gtaagccgcg
    10801 ttactgcttc gcagtataag gaaagccggg tatacacatc attaaacggc tatcgctttg
    10861 atcattttgc tccttacctg tatgtgagtg atgactatgg tacgaactgg aagcagatcg
    10921 gcaaggacct gcccaatgaa cccatcaatg tggtaaaaga agatccgaag aatgacagca
    10981 ttctatatgt gggtacagac ggcggcttat atgtaagctt tgatgccggc aatagtttca
    11041 tgctctggaa taacggtatg ccggaatctg taccggtaca tgatatcgcc atacaggcca
    11101 gggaaaatga gattgtactc ggtacacatg gccgaagttt gtatgttgcc agtctgaacg
    11161 atgtacacgc tgttaaaaaa gacccggagt ggatgaagaa aaaaccgaag ccgaagaaag
    11221 aggaaagacg gaatgaggaa gaggaggatg aaagcaaacc tgaaatggat taaaatagaa
    11281 aagtccggaa gtatgaactt ccggactttt tttatcagct tcattcagct ttcgaaataa
    11341 ctgaaggcaa atttcaattt aggatcagaa ctggtgaaac ctttcaaaac aggctttc
//
LOCUS       DAOPMW010000070        11391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6042_length_11391_cov_8.246824, whole genome shotgun sequence.
ACCESSION   DAOPMW010000070 DAOPMW010000000
VERSION     DAOPMW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11391
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_11391_cov_8.246824"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2928
                     /locus_tag="PLU11_05425"
     CDS             91..2928
                     /locus_tag="PLU11_05425"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58587.1"
                     /translation="MRRFFTFLFIISLASPLAAQKNGTVKGIAFDTLSKQNVGAATIT
                     VLERKDSSLVTFSMTGADGRFEIKGLANGDYRLLISHVNYHNSNKYFSINDSIKNADL
                     GTLVMNDKTKVLAEVVLENEAPPITLINDTIQYNAGSFKTAPNASVEQLLKKLPGVKV
                     EKDGTVKAQGETVTKVLVDGKEFFGNDPKVATKNLPADAVDKVQVYDKQSDQAQLTGF
                     EDGNYEKTINLKLKKDKKKGLFGKVNAGAGNKERYEGRFNVNSFKGARQFSAIGMGNN
                     TNAEGFSFMDILSFTGELSRMQRGGGGNININLSTSDASAMGYNGSNPNSGINTAWGG
                     GFNYNNIVGKKLDLQSNYFYNRFNPNTETHIQRQYLLPDTSYYNSNSYADNLNNTHRL
                     NLNLLYQLDSLNSIRVIPSFSYQQTRNRSNTDYQTMSKQQSMINDGYSNSISNSEGYN
                     FRNEIAWRRKFLRKGRTFSLTLQTTLNETDGDGSLSSINSFYQPNGGLSRKDTLNQQS
                     TTTGSLKGYTARAVYTEPIGKRTLMEFSIGKSNTRNLSEKLTYDYNKNNGKYDVQNAF
                     LSNDFENSYGFTNAGFRIRNQQKKYNFALGVNWQQADLDGKIISGTKDSLISKRFQNL
                     LPNARFQYNFTRFKNLSLTYNTNTNQPSMTQLQPVPDVSNPLNIRDGNPDLKQEYNHN
                     LQANLALISPFSSKNLFFFLRAQTAQNKIVNYDSLNQLTGVRKTKPVNVNGVYNLSAN
                     FSYSRPVRFLKGTVELSSNVGYNRGKQLINTLSSPMEENAIGVFSVGPELRLDMSPTE
                     KFNLVLGTSVNHNNTTYSLQPGLSNNYLSQEYSASVDWELPKGFFFSTDFTYTVNSQR
                     AAGFNLKVPLWNASISKQMLLFNRGELKFSARDLLNRNVGISRNTSNNFIEDSRVLTL
                     RQFFLLSFTYSLSKTGLNNAGRGGGMRVIAR"
     gene            complement(2942..3724)
                     /locus_tag="PLU11_05430"
     CDS             complement(2942..3724)
                     /locus_tag="PLU11_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPN58588.1"
                     /translation="MTIQADFAERVKKLVQEDPSVTGLAVAGSWITDEIDEYSDLDLI
                     LVTKYKIAGNKEKMMETARRFGDLVSGFTGEHVGEPRVLICLYDNPLLHVDIKFLIPD
                     EFKDRIENPVILFEREGQLSKIIHATTATWPQPDYQWIEDRFWTWIHYAAQKIGRGEL
                     MEAFDFLSFLRMTVLSPLLQVKNNRHARGLRKVETELNASDLENLKITIAQYNRESIL
                     KALDNTISIYKNLRRKLFDDKVQLQTRAEKLGTGYLKQVARQ"
     gene            complement(3734..4711)
                     /locus_tag="PLU11_05435"
     CDS             complement(3734..4711)
                     /locus_tag="PLU11_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPN58589.1"
                     /translation="MKQFLRKLIDVSTRVPQAAAAEGGNKISPYRYAKSYRELTITVR
                     RLFRDLFLMTAGIFSAAFGFKGFLLTNEFIDGGATGISLLISFLTKIPLYLLIITVNI
                     PFIILGYKIMGKAFAIKTMIAISGLALVLATVNFPNVTNDNLLVAIFGGFFLGAGIGL
                     SVRGGAVIDGTEILAIYLSRKLGTTIGDIIILINVLIFSSAAYFLSIEIALYSMITYL
                     AASKTLDFIIEGIEEYIGVTIVSSHSDEIRQMIIDKMGRGCTVYTGKKGYGKKGETKD
                     VDIIYTVITRLEINKLNTEIEKIEPDTFVVMSSVKDTKGGMIKKRPLKH"
     gene            4850..6937
                     /locus_tag="PLU11_05440"
     CDS             4850..6937
                     /locus_tag="PLU11_05440"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HPN58590.1"
                     /translation="MKPFPVLLLLFVFTQTALCQYHPVQQKAMVVKRMIELNHLSPRP
                     VDDSFSYRFFTSIINRADERRLFFTDAEFKSLQAFRLKLDDELNNREWIFYNQFSALY
                     QQAIKRADSILLKLTTKPFDFTLNETVSTGKKDDFVFAADLPALFSRWNRYLKFRALD
                     ALYDISSLDTAGKTGIKTVMAGNELRVREQLKKNEGRQLKRILEYSTGLEGYIMEIYL
                     DALAGCFDPHTSYFSPQGKQEFQEQLSSEALSLGLDLDENEKGQVIVDKLAPGGPAWK
                     SGDLNKGDVLLSMHWEGKENKDLTGAGLQEAYEEMEKVTEGKVILKVQKADGTIKTTM
                     LRKEKIQNEENVVKGFILEGEKKIGYILLPGFYTEWENETGSSCANDVAKEIVKLKKE
                     NIDGLILDVRYNGGGSVGEAMEMIGIFVDEGPEAGIKTKEPKIVYLKDPNRGTIYSGP
                     MLLLVNGQSASASEMLAASLQDYNRAVIVGSPTFGKATMQQMFPLDTTIKKPTQHDLS
                     RDMIKITTGKLYRLNGESAQKTGVLPDVVLPDAFDGIEYREKFKPNVLNSEPVAKNSY
                     YKPLPVLPVADLAAKSAARINADDNFKDIQRIIALMIARRDKQETISLKTEEYEQWAR
                     QNDKELASLKGTGLASGKLFRTGNHAADKSSFVNNEYAREINQQWLDEIAEDIYIQEA
                     FLVICDLIKLRTP"
     gene            6952..8004
                     /locus_tag="PLU11_05445"
     CDS             6952..8004
                     /locus_tag="PLU11_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58591.1"
                     /translation="MNIYFRSIPVLLLFCFLIRPALGMAQSFETPVEYLDYIGKANEQ
                     LTLKYMVYLSSISHGKSARKVEKRRLEVLDAIHNTRMNIMGMPPYKGDKSFRDTTVAY
                     LKILNSVFNEDFSKIVNMEEIAEQSYDGMEAYMLAQEKANEKLQEASKRQSETQKLFA
                     KKNNINLLENTSELEAKMKIASDVMHHYNEVYLVFFKSYKQEAYLMEAVAKKNVNGIE
                     QNINSLQTFAEQGLEKLKTLNPYTNDASLVTACRNMLNFYKEEAKKSAAISDFVLKEE
                     NFNKIKKKFDSKPSSSRTQQDVDEFNKAVNDMNAGINSYNNTNNELNKKRAQALDAWN
                     STVKRYMDTYVPQQRK"
     gene            complement(8094..8900)
                     /locus_tag="PLU11_05450"
     CDS             complement(8094..8900)
                     /locus_tag="PLU11_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPN58592.1"
                     /translation="MKKLLLCGMALTLTISTLTAQQTQGKVNYNRTVKLQMSFAGMPG
                     GLEQQLPSSRIDKYELVFGNNQSLWKQGEQEPEEEQSFGSEGMQIRMVVAGSNDVLYC
                     NFDKSRRVEKREMFDKTFIVDDTLTKLKWKMTSESKIILNHNCMKAEAVRISKRMTMN
                     MDNGKMERKEIDDTAKIIAWFAMDIPVSAGPSEFQGQLPGLILELDVRNGDQVYLATS
                     ITETTDLAVIKEPTGKKKYTQEEFKIERDKMLEEMNRNNGGGNRRVIRMN"
     gene            9016..10506
                     /locus_tag="PLU11_05455"
     CDS             9016..10506
                     /locus_tag="PLU11_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN58593.1"
                     /translation="MKRRLHIPAPFLMLISILGIAAFQVYWLQQNYDREKKALDVKAE
                     MQFRETVMRLQVAKLKLDGVSFDSAGKDGVRILVKNDGENRIRVRPREGMVSTINVIR
                     EKLNDSLRQGKTKPNRGVMISMETNSTFSEESDSLKLDMAFPARPPRPGNHMFNFLYG
                     VDSLQDTLRLAEIDSAYTLALKKQKLDIPFSIIRKEKTEETEHPKYSTVTIGLAHPVS
                     YTLQLGNTFPYLIRRIAQPILFSILLLGITIVAFLVLYRNLRKQRKLAELKNEFISNI
                     THELKTPIATVGVAIEALKNFNAIDNPERTREYLDISQNELHRLSLLVDKVLKLSMFE
                     NQGIELKYETLNLRGIVTEVVESLKLQLEKFKATVDVQYEGDTELRGDRLHLLSVVFN
                     LLDNALKYRSDNKQVKIDVQINGTADTVQLIIRDNGIGISPEYQGKIFEKFFRVPHGD
                     THNAKGYGLGLSYVAQVIKKHEGTITVQSVPGESTSFTITLPKSQS"
     gene            10503..11192
                     /locus_tag="PLU11_05460"
     CDS             10503..11192
                     /locus_tag="PLU11_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58594.1"
                     /translation="MNKTKVLYVEDELFLGKIVRESLESRGFDVLMEEDGAKVLERFK
                     KEKPDICVLDVMLPNKDGFTIADEIRELDEEVPIIFLTAKTQTEDVVKGFTLGGNDYI
                     RKPFSMEELIVRMQHLLRQKSEGPQKIQGNTVTMGKFSFQLNRQVLVIGTEEKKLSFR
                     ESELLKLLYENRDKIIDRRDILNLLWGNDSFFNSRNLDVYITKLRGFLKADPSLEIIT
                     IKGVGYRFVAG"
BASE COUNT         3303 a         2503 c         2713 g         2872 t
ORIGIN      
        1 ctatacatta accataatta accttaaacc cactccattt aacccgtttc tacaatcggt
       61 acgactattt tcgtggcaga aaccagagcc atgagaagat tttttacatt tttatttatt
      121 atcagtcttg ccagtccgct tgctgctcag aagaatggga ccgtaaaagg aattgccttt
      181 gataccctca gtaagcaaaa tgtgggagcg gctaccatta ctgtgctgga acggaaagat
      241 tcctcactgg tcactttttc catgaccgga gccgatggcc gttttgaaat taaaggactg
      301 gccaatgggg attatcgcct cctgatcagc catgtcaact accacaacag caataaatac
      361 ttctcgatta atgattccat caaaaatgcc gacctgggca ccctggtgat gaatgataag
      421 accaaggtac tggccgaagt ggtgctggaa aacgaagccc caccgatcac cctgatcaat
      481 gacacgatcc aatacaatgc tggttctttt aaaactgcac cgaatgcaag tgtggagcaa
      541 ttgctgaaga aactgccggg agtgaaagtg gaaaaggacg gcactgtgaa agcacagggc
      601 gagacagtaa ccaaggtact cgtggatgga aaggagtttt tcggcaatga tccgaaagta
      661 gccactaaga acctgccggc cgatgcggtg gacaaagtac aggtgtatga caagcagagt
      721 gaccaggctc agctgacagg ttttgaggat gggaattatg aaaagaccat caaccttaaa
      781 ctgaagaagg ataaaaagaa aggcctgttt ggaaaagtaa atgcaggggc cgggaataag
      841 gagcgatacg aaggccggtt caatgtaaac tcttttaaag gggcccgcca gttttcggca
      901 atcggcatgg gcaataatac caatgcagaa ggattttcct ttatggatat cctgagtttc
      961 accggtgaac tgtcccggat gcagcggggt ggtggtggaa atataaatat caacctgtct
     1021 acatcggatg cctcagcgat gggttacaac gggagcaacc ccaacagcgg catcaataca
     1081 gcatggggcg gcggattcaa ttacaataat atcgtcggga aaaagcttga cctgcaaagc
     1141 aattactttt acaaccgctt taatccaaat accgaaactc atattcagcg acagtatctt
     1201 ttaccagata cttcttatta taattccaat tcctatgccg acaacctgaa caatacacac
     1261 cggctcaacc tgaacctgct gtatcagctg gattcactga attcgatccg ggtaatccct
     1321 tccttcagtt accagcaaac ccggaaccgg tccaatacgg attaccagac gatgtccaaa
     1381 cagcagtcca tgatcaatga cgggtacagt aacagtatca gcaacagcga gggctataat
     1441 ttccggaatg agatagcctg gcgccgcaaa ttccttcgta agggcagaac tttttccctg
     1501 accctgcaaa ccacgctgaa tgaaacagat ggtgatggca gtctttcgtc gatcaatagt
     1561 ttttatcaac ccaatggcgg tctgagccgg aaggacaccc tcaatcagca aagtaccact
     1621 acaggcagcc tgaaaggata tacggccagg gcggtttata cggaaccaat aggcaagcgg
     1681 acattgatgg aatttagcat tggaaaaagt aatacccgga atctctcgga gaaactgact
     1741 tatgattata ataagaacaa tgggaagtat gatgtacaaa atgcttttct gagtaatgat
     1801 tttgaaaaca gctacgggtt taccaatgcc ggtttccgga tccggaacca gcaaaagaaa
     1861 tacaatttcg ccctgggggt caactggcag caagcggatc tggatggaaa gattatcagt
     1921 ggcaccaagg attcgctgat cagcaagcgg tttcagaacc tgttgcccaa tgcccgtttc
     1981 cagtacaatt ttacccgctt taagaatctg tcgctgacgt ataacaccaa taccaatcag
     2041 ccatcgatga cccagctgca gccggtaccg gatgtaagca atccgctgaa tatccgggat
     2101 ggcaaccccg acctgaagca ggagtataac cataacctgc aggccaacct ggcgctgatc
     2161 agtcctttca gcagcaagaa cctgtttttc ttcctgcggg cccagacagc acagaataag
     2221 atagtcaact atgattcgct gaatcagctg accggggtac gtaagacaaa accggtgaat
     2281 gtaaacgggg tgtataatct cagcgccaat ttcagttaca gccgtccggt ccgattcctg
     2341 aagggcactg ttgaactcag cagcaatgtc gggtacaacc ggggtaagca actgattaac
     2401 acgctcagca gcccgatgga agagaatgcg attggtgtat tcagtgtggg ccccgaactg
     2461 cgcctggata tgagtccaac tgaaaaattc aacctggtgc tcggaacatc cgtgaatcat
     2521 aacaacacca cgtattcgct gcagcccggc ctcagtaata attatctgtc acaggagtac
     2581 tctgcttctg ttgactggga attgcccaaa ggatttttct tcagtacgga ttttacctat
     2641 acggtcaaca gtcagcgggc cgccggcttt aacctgaaag tgcctttatg gaatgcaagt
     2701 atcagcaagc agatgctgct ctttaatcgc ggggaactga agttcagtgc ccgcgacctg
     2761 ctcaaccgga atgtgggcat cagccgcaat accagcaaca actttattga agactcgagg
     2821 gtactcacgc tgcggcagtt ctttttgctg agttttacgt atagtctgag taagactgga
     2881 ttgaataatg cgggccgtgg tgggggtatg cgggtgatag cgcggtaaga aagcgcatta
     2941 cttattgccg ggccacctgt ttcaaatagc cagtacccag tttttctgcc cgtgtctgta
     3001 attgcacttt atcatcaaat agtttccggc gtagattttt ataaatactg atagtattgt
     3061 caagggcttt caggatggat tcccggttgt attgggcaat ggtgattttc aaattttcga
     3121 gatcggaagc attgagttct gtttccactt ttcgaagtcc ccgggcatgg cggttgtttt
     3181 ttacctgtaa taaaggagag agcacggtca tccgcaggaa ggaaagaaaa tcaaaggctt
     3241 ccattaattc tccgcgaccg attttctggg cggcataatg aatccaggtc cagaaccggt
     3301 cttcgatcca ctggtaatcc ggttgtggcc aggttgccgt ggtagcatga ataattttgc
     3361 tgagttgtcc ttcccgttca aacaggatta ccgggttttc gatacggtct ttaaattcgt
     3421 ccgggatcag gaatttaata tcgacatgta agagcggatt gtcatacaga caaattagca
     3481 ccctgggttc gcccacatgt tcgccggtga agccggatac gagatcacca aaacggcggg
     3541 ccgtttccat catcttctct ttattccctg caattttata tttggtcacc aggatcaggt
     3601 ccaggtccga gtattcgtct atttcatcag tgatccagga gcccgctaca gccagaccgg
     3661 ttacagaagg atcttcctgt accagttttt ttacccgctc cgcgaaatcc gcctggatgg
     3721 tcatagtttg atgttaatgt ttcagcggcc tttttttgat cataccgccc ttggtgtctt
     3781 ttaccgagct catcacgaca aatgtgtcag gttctatttt ttcaatttcg gtattgagtt
     3841 tatttatttc gaggcgggta attacggtat agatgatatc cacatctttg gtttcccctt
     3901 ttttgccata tccttttttg ccggtgtaaa cggtacaacc ccgtcccatt ttgtcaataa
     3961 tcatttgccg gatctcatca ctatgcgagg atacaatagt gactccgata tattcttcaa
     4021 taccttctat gataaagtcg agcgttttgg aagctgcgag ataggtgatc atggaataaa
     4081 gggcaatctc tatggagagg aaataggcgg cagaagaaaa tatcaggaca tttatcagga
     4141 ttattatatc cccgatcgta gtacccagct tccggctcag gtaaatcgcg agtatttcag
     4201 tgccatcgat taccgctccg ccccgcacgc ttaatccaat accagctccg aggaagaatc
     4261 caccgaaaat ggccaccagg agattgtcat tggtcacatt cggaaaattc accgtggcca
     4321 gtacgagggc caggccactg atggcaatca tggtcttgat agcgaaagcc tttcccatga
     4381 ttttatagcc caggatgata aacgggatat taacggtgat gatcagcagg taaagcggaa
     4441 tctttgtgag aaaggagatc agcagggaga tacccgtggc acccccatca ataaactcat
     4501 tggtaagcag gaaacccttg aaaccaaagg ccgcggagaa tatacccgcg gtcatcaaaa
     4561 acaggtccct gaataggcgc ctgaccgtaa tggtcagttc ccggtaggat tttgcatacc
     4621 ggtaaggcga aatcttattt ccaccttctg ccgctgccgc ttggggaaca cgggtactta
     4681 catctataag tttccttaag aattgcttca ttcgttagag atcgttgata ccttcaaatt
     4741 taccgaaatc cggccgggaa gcgtataaaa tcaggaataa ttcaggccgg ggttaatctt
     4801 taaataatat tatttaatta ttttcataac tcttcagctt ttaactgtta tgaaaccttt
     4861 tcctgttctg ctcctcttgt ttgtttttac acaaacagcc ctttgtcaat accatcctgt
     4921 acagcaaaaa gcgatggtag taaaaaggat gattgaactg aatcatcttt cgccccggcc
     4981 ggtggatgat agtttttcgt accggttttt tacttccatt attaaccggg ccgatgaacg
     5041 tcgtttattt tttaccgatg ctgagtttaa atccctgcag gccttccgcc tgaaactgga
     5101 tgatgaactc aataaccggg aatggatttt ttataatcag ttttcagcct tgtaccagca
     5161 agcaataaaa agggcagatt ccattttatt aaaactgact acaaaaccgt ttgattttac
     5221 attaaatgaa acagtcagta ccggtaagaa agacgatttt gtttttgccg cagacctgcc
     5281 ggccctattc tcgcgctgga accgctatct caagttcagg gcacttgatg ctttatatga
     5341 tatcagctcc ctggataccg ccggaaaaac cgggattaaa acagtaatgg ccggaaatga
     5401 attgcgggta cgggaacaac tgaaaaaaaa tgaaggccgg caactgaaaa gaatacttga
     5461 gtacagtact ggcctggaag gctatatcat ggaaatctac ctggatgcac tggcaggttg
     5521 ttttgatccg cataccagct atttttcgcc acaaggcaaa caggaattcc aggaacagct
     5581 gagttcggag gcgttatcgc tgggactgga cctggatgaa aatgaaaaag ggcaggtaat
     5641 cgtggataaa ctggcacccg gaggcccggc ctggaaatcc ggggacctga ataaaggcga
     5701 tgtactgctg agtatgcatt gggaagggaa agagaataaa gacctgaccg gtgctggttt
     5761 gcaggaagcc tatgaagaaa tggaaaaagt taccgaaggg aaagtgatac taaaagtgca
     5821 gaaagcggat ggtaccatta agacgacgat gttgcgtaag gaaaaaattc agaatgagga
     5881 aaatgtggtg aagggcttta tccttgaggg agagaaaaag atcgggtata ttttgttacc
     5941 cggtttttat accgaatggg aaaacgagac cggttccagt tgtgccaatg atgtggcaaa
     6001 ggaaattgta aaacttaaga aggaaaatat agacgggctg atccttgatg tgcgctataa
     6061 tggaggcgga tcggtcgggg aggctatgga aatgattggc atatttgtag atgaaggacc
     6121 ggaagcaggg attaaaacaa aggagccaaa aatagtgtac ctgaaagacc ccaacagggg
     6181 aaccatctat tccgggccca tgctgttact ggtgaacgga caaagcgctt ctgcttctga
     6241 aatgctggcg gcttcgttac aggattataa tcgcgcggtt atcgtaggca gcccaacctt
     6301 tggtaaagca acgatgcagc aaatgtttcc gttggatacc acgatcaaaa aaccaacaca
     6361 acacgatctg agccgggata tgatcaagat aaccacgggg aaactataca gactgaacgg
     6421 ggagtccgcc cagaagaccg gtgtgctgcc ggatgtggta ctgcctgatg cttttgacgg
     6481 gatcgaatac cgggagaaat tcaaacccaa tgtacttaac agtgaaccgg tggcaaagaa
     6541 cagttattat aaaccgttgc cggtattgcc ggtggccgat ctggcagcta aaagtgcagc
     6601 caggattaat gcggatgata attttaaaga tatccagcgg atcattgccc tgatgattgc
     6661 acggcgtgac aaacaggaaa ctatttcact gaagacagaa gaatatgagc aatgggcccg
     6721 gcagaatgat aaagagctgg cctccttaaa aggaaccggc ctggcctccg gaaaactgtt
     6781 caggaccggg aatcatgccg ccgataaaag ctcttttgta aataatgaat atgcccggga
     6841 gatcaaccag caatggctgg atgaaatcgc ggaagacatt tatattcagg aagctttcct
     6901 ggtaatttgt gatttaatca aactgcgcac tccttaacta aaccatataa tatgaacatt
     6961 tatttcagat ccattcccgt tttattactt ttttgtttcc tgatccggcc cgcactgggt
     7021 atggcccagt cttttgaaac acctgtggaa tacctggatt atatcggcaa ggccaatgag
     7081 caactgaccc tgaaatatat ggtctatctc agcagtattt cacacggaaa aagtgcgcgg
     7141 aaagtggaga aacgccggct ggaagtcctg gatgccattc ataatacccg catgaatatt
     7201 atgggaatgc ctccgtataa aggcgataaa tccttccggg ataccacagt agcctatctg
     7261 aaaatcctga acagtgtgtt caatgaggac ttcagcaaga ttgttaatat ggaagaaatc
     7321 gcggagcaga gctatgatgg aatggaagcc tatatgctgg cacaggaaaa agccaatgaa
     7381 aaactccagg aagccagtaa gcggcaaagt gagacccaga aactttttgc caaaaaaaat
     7441 aatatcaatc tgctggagaa tacgtctgaa ctggaagcca aaatgaaaat tgcttcggat
     7501 gtgatgcacc attacaatga agtatacctg gtttttttca aaagttataa acaggaagcg
     7561 tacctgatgg aggcagtggc taaaaagaat gtcaacggca ttgaacagaa tatcaactcc
     7621 ctgcagactt ttgcggagca ggggctggaa aagttaaaaa ccctgaatcc gtataccaac
     7681 gacgcatcgc tggtgacggc ctgccggaat atgctcaatt tttataagga agaagcaaag
     7741 aaaagcgccg ctatcagcga ttttgtattg aaggaagaaa atttcaataa aattaaaaag
     7801 aagtttgatt ccaagccatc ctccagccgc acccagcagg atgtggatga gtttaataaa
     7861 gcggtaaatg acatgaatgc aggtattaac agctacaaca ataccaataa cgaactgaat
     7921 aaaaaaaggg cacaggccct cgatgcatgg aacagtaccg ttaaaagata tatggatacc
     7981 tacgtaccgc aacagcggaa atagaatggg tttatgtaaa aagcctgtct ccttcataat
     8041 gcggctttcc gcaggttcag gagacaggct tttgacttat cgcttaatca acatcagttc
     8101 atccgtatca cccttcggtt gccaccgccg ttattccggt tcatttcttc cagcatttta
     8161 tcccgctcga ttttaaattc ttcctgcgtg tattttttct tgccggtggg ttctttgatt
     8221 acggcgaggt cggttgtttc ggtaatactg gtggccaggt acacctggtc gccattgcgc
     8281 acatccagtt caaggatcag acccggcagt tgtccctgga attcagaggg accagcggat
     8341 acgggaatgt ccatcgcaaa ccaggcaatg atttttgcag tatcatctat ttccttcctt
     8401 tccattttgc cattgtccat attcatggtc atgcgtttgc tgatccgcac ggcttccgct
     8461 ttcatgcaat tatggttcag gatgattttt gattcactgg tcattttcca tttcagtttg
     8521 gtgagggtat catccacaat aaaggtcttg tcaaacattt cccttttttc cacgcggcgg
     8581 cttttgtcaa aattgcaata cagtacatcg ttgcttccgg ctaccaccat ccggatctgc
     8641 atgccttcgc ttccgaagga ctgctcttct tcaggttcct gttcgccttg tttccagagc
     8701 gactgattat ttccaaaaac cagttcatat ttatcgatcc ggctgctggg caattgctgt
     8761 tccagtccac ccggcatacc ggcaaaactc atttggagct taacggtccg gttatagttt
     8821 acctttccct gagtttgctg ggcggtaagg gtagagatgg tcagtgtcag cgccattccg
     8881 cataaaagca attttttcat ttgtttacta tttgccccaa agctaaataa cgggaggtta
     8941 aaccaccttt taataaggtt aataaaggtt aacggggtta aaccaccccg tgataaagac
     9001 tatttttgtg tcgtaatgaa acgccgcctg catataccag ctcccttcct gatgctgatt
     9061 tccattctgg ggattgccgc tttccaggta tactggttac agcagaatta tgaccgggaa
     9121 aaaaaggcat tagatgtaaa agcagaaatg cagtttcgcg agacggtcat gcggttacag
     9181 gtggcaaagc tgaaattgga tggagtaagt ttcgacagtg ccggaaaaga cggagtgcgg
     9241 attttagtca agaatgatgg ggaaaacaga atacgggtaa gacctaggga agggatggtt
     9301 tccactatca atgtcatccg ggaaaaactg aatgactctt tacgccaggg caaaacgaaa
     9361 cccaacagag gtgtgatgat ttccatggag accaattcaa ctttttcgga agagagcgat
     9421 tccctgaaac tggatatggc attcccggcc cgtccccccc gcccgggcaa ccacatgttt
     9481 aactttcttt atggtgtcga ttcactacag gataccttac ggcttgcaga aatagattca
     9541 gcctatacac tggcattaaa aaaacaaaaa ctggatattc cattttccat tatccggaag
     9601 gaaaaaacgg aagaaacgga gcatccgaaa tacagtacgg tcacgatcgg actggcacac
     9661 ccggtttctt atacgttaca acttggcaac accttccctt acctgatccg ccgcattgcg
     9721 caaccgattc ttttttccat actcttattg gggattacca ttgttgcctt tctggtgttg
     9781 taccggaatc tccgcaaaca acgcaaactg gccgaactca agaatgagtt tatcagcaat
     9841 ataacccacg aattaaaaac cccgatcgcc actgtagggg tagccattga agcgctgaaa
     9901 aatttcaatg ccattgataa tccggagcgt acgcgggagt acctggatat ttcgcagaac
     9961 gaactgcacc ggctcagtct gctggtggat aaagtgctca aactgtcgat gtttgagaac
    10021 cagggcatcg aactgaaata tgaaacactg aatctgcgtg gtatcgtaac cgaggtggtg
    10081 gaatcattga aactgcaatt ggagaaattc aaggcaaccg ttgatgtaca atatgaagga
    10141 gatacggaat tgcggggtga ccggcttcac ctgctgagtg tggtgtttaa cctgctggac
    10201 aatgccctga agtaccgcag tgacaataaa caggtaaaga ttgacgtgca gatcaacgga
    10261 acggccgata cggtacaact gatcatccgt gataacggaa ttggaatttc acctgaatac
    10321 caggggaaaa tatttgaaaa attcttccgg gtgccgcatg gcgacaccca taatgccaag
    10381 ggctacggac tgggcttaag ctatgtcgcc caggtgatca aaaaacatga agggacgata
    10441 accgttcagt cggtacccgg tgaaagcaca tcgtttacca tcactttacc aaagagtcag
    10501 tcatgaacaa gaccaaagta ctatatgtgg aggacgaact gtttctgggg aagatcgtca
    10561 gagagagtct cgaaagccgt ggctttgacg tgttgatgga agaagatggc gccaaagtac
    10621 tggagcggtt taaaaaagag aaaccggata tctgtgtact ggatgtgatg ttgcccaata
    10681 aggacggctt taccattgcc gatgagatcc gcgaactgga tgaagaagtg ccgatcatat
    10741 ttctcacggc caaaacccag accgaagatg tggtaaaagg atttaccctt ggcgggaatg
    10801 attatatccg caagcccttc agtatggaag aactgattgt acggatgcag cacctgctgc
    10861 ggcagaaatc agaagggccg cagaagatcc agggcaatac ggtgacgatg ggaaaattca
    10921 gtttccagct caaccggcag gtgctcgtaa tcggtaccga agaaaagaaa ctctctttcc
    10981 gcgaaagtga attactgaaa ttattatacg aaaaccgcga taagatcatt gaccgccggg
    11041 atatcctcaa cctcctctgg ggcaatgatt ccttcttcaa cagccgcaac ctggatgtgt
    11101 atatcactaa actgcggggc tttctgaaag cggacccttc tttagaaatt attacgataa
    11161 aaggggtggg gtatcggttt gttgcgggat agaaagaaag aaccaagatg ccgagaggac
    11221 aaagaaacca agaaccaaga taccaagacg ccaaagaaat atcaaatgac aaatttcaaa
    11281 aaagcatcaa aaggaaataa tcaatgcctt ctgtgaaagt tctcaggatg attggagctt
    11341 ttttggagtt tctcttttgg tccttctttg atccttgaat tttggttctt c
//
LOCUS       DAOPMW010000071        11349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6067_length_11349_cov_6.334160, whole genome shotgun sequence.
ACCESSION   DAOPMW010000071 DAOPMW010000000
VERSION     DAOPMW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6067_length_11349_cov_6.334160"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..1925)
                     /locus_tag="PLU11_05465"
     CDS             complement(468..1925)
                     /locus_tag="PLU11_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58595.1"
                     /translation="MIDEIVKIHDKNSAEIKVGFVARKKQKHNFFSLNFWMYIPNSLD
                     INRFTYSKDDFYRDMKSNIRLITPVYILRDLAEGDHSAFIFLEKSFNNLASQRTRTHI
                     ADFEYQIKMFLSILKSSLREEIAHILQAPEADLDYLVYNFQHFNQVITGKYRDLYRII
                     NVPTIDKKLMDYFRFGDEFMSNTIEYHAFRLMQGLDKRDARIFKQYKADLTEFVNKEI
                     NYKKEKKFPIAGKENRDANNELVYRLGMLKKYAESHLFLNIDKRKDGVVTEQLLYSLA
                     AGISMVFATVIAFSVQQKFGNYTMPLFVALVVSYMLKDRIKDFARYYFAHKMGSRYYD
                     HKISMNINRKDIGWAKESMDFISINKVPDSITKCRNNAEFFEQNNPTNKEKVLLFRTR
                     MYINREKLDTTSDYSISGINSIIRLNLLQLMRNMDNADFPLFCPDPEHGFDVIYGEKL
                     YYINIVVEKINDEQKELKRYRLSVNSKGIQQISPL"
     gene            complement(1944..3557)
                     /locus_tag="PLU11_05470"
     CDS             complement(1944..3557)
                     /locus_tag="PLU11_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HPN58596.1"
                     /translation="MNTTDPRYNPKAFDPLDMNNYRVEKLKKMEKKGVEKWMEWLGGP
                     LAILSFILIYYFVDISFLNQIKTEVLRDDSLKRLKEIGAENFSRINYAMLGIFVASII
                     LWITEAIPNYLTSLIVVIAMVLTGVTTENLAYAQLGHPVMWLNILSFILASMLVTTKA
                     AKRFALWFVLKFGKNATWIFFSFIVINIVLSVFISATTAKATILLPIFMVIAAIYGAT
                     ADHRNNFGRSIVLENLFQINIGASGFITGSGANLLAGSLIAGALGQSLFGFQDWFIAA
                     FPLCIILILIGWVVGTRVIFPMKKEEQRPQIEGGMQRLKEEYLKLGKMTVNEYKSIFI
                     FITVLALWATDKQHGISQTAVAFLGAVVALLPRVGVVKWNDVDIPWHLMLFSAGAYTL
                     GAGLEATDLPATLVDALFGYFGISKATPFWILYLGLTFLMLFSAILFQSKTMRALIFV
                     PMAIGIAQKFGYPVMSLAFPVALLIEHVYVLPFNSKPSALLYTTNQYSMSDSFKFGFT
                     MMVISWFMIIIWGETVLRWLGYTSNGVFF"
     gene            complement(3585..4340)
                     /locus_tag="PLU11_05475"
     CDS             complement(3585..4340)
                     /locus_tag="PLU11_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HPN58597.1"
                     /translation="MKWNSAIWISGAVSILLVSGCRKDNRGFGPQDKEYEEYNTGISG
                     FSGLCFSATKSSFYAISDQGSIYEINTDGSTIRQLPVTGQYDFEAITLDAVNNRLFVA
                     DESSMNLYGLTPNQSSLNLVTHINIPGGVSNKGIEGLAYGQDTLYIVNQESPTLLIKY
                     SLASQAEVQRILVDFASYLSDIFFDASDNSLWICDSQQQLLFHCTLNGNVLASQEIDF
                     VPKAEALVIDRANQTAWIGCDQSSKLFKVKLKI"
     gene            complement(4414..4884)
                     /locus_tag="PLU11_05480"
     CDS             complement(4414..4884)
                     /locus_tag="PLU11_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPN58598.1"
                     /translation="MAQEIEKKFLVTSDAFKTEAAKETRITQGYLSSVPERTVRVRIK
                     GKAGFITIKGKGNDSGASRYEWEKEIPVSEVEELLNICEPGVIDKIRYEVKAGHHTFE
                     VDEFYGENQGLVVAEVELQAENDHFEKPAWLGEEVTGDVKYYNSMLMKNPFTKW"
     gene            complement(4898..5539)
                     /locus_tag="PLU11_05485"
     CDS             complement(4898..5539)
                     /locus_tag="PLU11_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58599.1"
                     /translation="MSKEELKIGEISKPRFEFRSFGQDFEKAAQRMARLSMPVPEKVW
                     ERQSDEIYILSRKNDINNTKIRDGKMDIKTFVQTVDGMEQWNPLMKGSFPIAAEVLLK
                     EVFPAFQVTAPVLDKETYSYDEFIGMINAHPDLQAVRVHKQRFGYMVNNTICEVGNVL
                     INGAKVVTINSESTEVEDIKKTVADIGLDGVENINYLQAIKRVTGMISQPLAN"
     gene            complement(5607..6473)
                     /locus_tag="PLU11_05490"
     CDS             complement(5607..6473)
                     /locus_tag="PLU11_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPN58600.1"
                     /translation="MKLTYFISSLIGLALFASSCVKDEIYNGPATISNVAFTPSTVTP
                     DDVVNVTATVTSVKGIEAVTLFYKVNGGTALSVPMVAGASNSYSGSIPKQVDQTTIVL
                     YVGAKTTGGIENSSAEKTYKVGAIPPNYAAIVLNEIDGNTKFVELFNNSAAPVNISGM
                     VILKNTVSLKNPDGTPWSVPAATILPANGYGIIKCSGYTATADPAALIIGTVGDGMSA
                     KQTLSLELVKPDGTSVSLFTRGAAPWGVTISAIPATDSYSRVPNATGTWKLALQTPGK
                     VNGASTGDIPAL"
     gene            complement(6495..7853)
                     /locus_tag="PLU11_05495"
     CDS             complement(6495..7853)
                     /locus_tag="PLU11_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPN58601.1"
                     /translation="MKRILIIVAVLLSMHMAIAQDTSRQDRFNQYGRLVSRSPLDVEN
                     RNGILVFESKDQDYRLWFDIRVQVDGQIFSNKTMNPIGNGVSIRRARFAAKTNLTKNW
                     YGEIDLDFSNGILELNDAYIQYDFRNGLATRVGNFKERFSMSQTSSSRYLNFLERAMV
                     VTAFTPSRHVGWETSYSGTHFLAAGGVFFQEVEDAEMRTFVEDNNKDYGRDEGVSFTG
                     KFALQPTKADKDFGGHLAYAASYRQPKTSVEAAEYGGLRYSTRSLSSINRKKYLDTDI
                     IPNVNHTVLNNIEIAGYFKGFGFQSEYIRSKVIRNNNLSTLNFNGFYAQAACLLFGGR
                     QFYNHTEAEFSQPYRGKKWGDMELAFRYDNIDMNDPALFGGAAEAYTAGVNIYAAKNV
                     RFQINYSYVNHDRYASGKKKLFVGYDLAGALTKDPTKVADANGTAGEDYGMMGVRFEI
                     NF"
     gene            complement(7871..9619)
                     /locus_tag="PLU11_05500"
     CDS             complement(7871..9619)
                     /locus_tag="PLU11_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPN58602.1"
                     /translation="MLFLLFITSGLFLGWSLGANDAANVFGTAVGSRMIKFRKAAIIA
                     SLFVILGAVLQGTGATETLSSLGSIDAMGGAFTVALCSAIVVTWMTYYKLPVSTSQAI
                     VGAIIGWCFYTSNPVDYKVLTVIAVSWFTGPLLGAIFSGLLYLLMRYYMRRTKTHVLI
                     LDSHIRNGLLIVGAFAAFSLGANNVSNVMGVFVNSTSFHINAGPFTFTNTHVLFLLGG
                     LSIAAGILTYSKKVINTVGHGLLEMTPETAIVVVLSQALVLFIFSSTALSDLFVRIGL
                     PAIPLVPVSSTQVIVGALIGIGAIKGIQEIRFKMLGTIMLGWLMTPVLAGLLTFISLF
                     FVNNVFKIDITHKQLTVVTSPAAGPTTTVINLPVNTVLISVTAIALAVMGITAYLYFR
                     HYRSKTAAREYNTRLNEQLQYAELQKSLSEIEIKQVQVENSSLHNRLEEKRKELVNYA
                     LNIADQRQFLELTCAALEECCRESDPQKKNSQIREVIRSVKQKMSFTDEIEQIYQQAE
                     HVNDEFPSRLTELSPHLSAQERKLALLLRVGFSSKEIAPLLNISTKSVEIARYRLRKK
                     FDLKKEINLTQFIKTI"
     gene            complement(9624..10268)
                     /locus_tag="PLU11_05505"
     CDS             complement(9624..10268)
                     /locus_tag="PLU11_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPN58603.1"
                     /translation="MFFTKANKSIALIDSFFNCVDQSVLLFKEGVKNYLYKNTENFNN
                     NLQSLANLEIESDQIRRKIENTLYTQSLMPQLRGDILRLLEELDNLIDLSKKNLFQFD
                     VEIPMVPQELVQDFVKLTQISVSAAESVIPAAKSYFNEPSTVNEKIHRVFFFEKETDV
                     YADVVRRKIFREMPSLKLSEKFHLRYFTLHIENISDTAEKVADLLAIMAIKRIM"
     gene            10743..>11349
                     /locus_tag="PLU11_05510"
     CDS             10743..>11349
                     /locus_tag="PLU11_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN58604.1"
                     /translation="MNDKLVQRIAAEVEDIKSAGLFKTERIIESPQGAEIIVNGKAVL
                     NFCANNYLGLSSHPKVIQAAHEAIDKRGYGMSSVRFICGTQDIHKELEEKISRFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDALNHASIIDGVRLCKAQRFRYEHNDMNDL
                     EVKLKEAAGCRSRIIVTDGSFSMDGTIARLDEIVKLAEQYDA"
BASE COUNT         3033 a         2577 c         2548 g         3191 t
ORIGIN      
        1 aacagcctgg tgtggtaaag gtaataatca ttggtgctgt acaaatagta cgttttaagg
       61 gaaggaatgt tacagcccgg caaaaaaaaa tccggatctg tgacccggat ttgtataaaa
      121 gtacttgtat ttcaattaat taactggaga aaccaggatg aactggcggg agaggttttc
      181 cagcatcagg tcatcagatg tgttattccc cggctcggaa caggctgttt tttctaaaca
      241 gggtgctgga gcgggtgctg gtgtttcaac cggacgcgtg ggaatgctta aaatcaggac
      301 agtagataat aataaaaggg caaaaggtat aaaggaaaac cttgaattcc gttgcatggt
      361 gatggttttt gagtaggacg aaggtaaacc cgggtatgtt acaggtttgt caattttttt
      421 cagtttgtca ttttatatta tatggaccaa gcctttgtga gcagtggtta taacggacta
      481 atttgctgta tacccttact gtttactgac aaccggtatc ttttcagttc tttttgttca
      541 tcatttatct tctccaccac gatattgatg taatacaatt tttcgccata aatcacgtca
      601 aatccatgct caggatccgg acaaaaaagc ggaaaatcgg cattatccat attcctcatc
      661 agttgaagca ggttgagccg gataattgaa ttaattccgg aaatggagta atcactggtg
      721 gtatccagtt tttcccggtt gatatacatg cgggtcctga acagtaacac tttttcttta
      781 ttggtcggat tgttctgctc aaaaaattct gcattgttcc ggcatttagt aatggaatcg
      841 ggtactttat tgatggagat aaagtccata ctctctttgg cccagccgat atctttccgg
      901 tttatattca tggagatttt gtggtcataa tacctggatc ccattttatg ggcaaaataa
      961 taccgggcaa aatctttgat ccggtcttta agcatatagc tcacaaccag ggctacaaac
     1021 aagggcatgg tataattgcc gaatttctgt tgtaccgaaa acgcgatcac cgtagcaaag
     1081 accatggaaa ttccagctgc caggctatag agaagctgct cggttaccac cccgtcttta
     1141 cgtttatcga tattcagaaa taaatggctt tccgcatatt tcttcaacat acccagccgg
     1201 tataccagct cattgttggc gtcccggttt tcttttccgg caatgggaaa tttcttttct
     1261 ttcttatagt tgatttcttt gtttacaaat tctgttaagt ccgctttgta ctgtttaaaa
     1321 atccgggcat ctcttttgtc gagtccctgc atcagccgga atgcatggta ttcaatcgta
     1381 ttgctcataa actcatctcc gaaccggaaa taatccatca gctttttatc aatggtgggg
     1441 acattgataa tccggtacag atccctgtat ttgccggtga tcacctggtt gaaatgctga
     1501 aaattatata ccaggtaatc cagatctgct tcaggagcct ggagtatatg ggcaatttct
     1561 tcccgcaggg aacttttgag aatggaaaga aacattttta tctggtactc aaaatcggcg
     1621 atatgggtcc ttgtccgctg tgaagccagg ttgttaaagg atttctccag gaagataaat
     1681 gccgaatgat caccttcggc cagatcacgg agtatatata ccggggtgat caggcggata
     1741 ttcgatttca tatcccggta aaaatcatct ttactgtagg taaagcgatt gatgtcaaga
     1801 ctattgggga tatacatcca gaaattaagc gagaaaaagt tgtgtttctg tttttttcgg
     1861 gccacaaagc caacctttat ctctgccgaa tttttatcgt gaatttttac aatttcatca
     1921 atcatttgag caggttactt gaattagaaa aataccccgt tactggtata acccagccag
     1981 cggagtacgg tttcgcccca tattatgatc ataaaccagc tgatcaccat catcgtgaat
     2041 ccgaatttaa aagagtcgct catactatac tgattcgtgg tatagagcag cgcggatggt
     2101 ttactgttaa atggtaatac atacacatgt tcaatcagca aagccaccgg aaaagcaaga
     2161 ctcattaccg ggtagccaaa tttctgggca atgccaatag ccatcggaac aaagatcagg
     2221 gcccgcatgg tcttcgactg aaaaaggata gcactgaaaa gcatcagaaa agtcagcccc
     2281 aggtagagaa tccagaaagg agtggctttt gaaataccaa aatagccaaa taaagcatct
     2341 accagggttg ccggcagatc agtggcttcc agtcctgcac ccagcgtgta ggcaccggca
     2401 gaaaataaca tcaggtgcca gggtatatcc acgtcattcc acttcaccac accgacccgg
     2461 ggtagcaaag ccactacggc accaagaaaa gcgacggctg tttgggaaat tccgtgttgt
     2521 ttatcggtag cccataaagc cagtacggtg atgaaaatga agatcgactt gtattcgttc
     2581 acggtcattt tgcccagctt cagatactcc tctttcaggc gttgcattcc gccttcgatc
     2641 tggggccgct gttcttcttt tttcatcggg aatataaccc tcgtacccac cacccagcca
     2701 atcagaataa ggataataca tagcgggaaa gcggcgataa accagtcctg gaaaccaaaa
     2761 agcgactggc ccaacgcacc ggcaattaat gatccggcga gcaggttagc cccggagccg
     2821 gttataaatc cgctggcgcc gatattaatc tggaaaagat tttccagtac aatgctcctt
     2881 ccgaaattat tccgatgatc ggccgtagcc ccatagatgg cggcgatgac catgaaaatt
     2941 ggcagcagaa tggtggcttt ggcggtagtg gcggagatga acaccgacaa aacaatatta
     3001 atgacaataa agctgaagaa aatccaggta gcgtttttcc cgaatttcaa tacaaaccat
     3061 aaggcaaaac gcttggcggc tttagtggtt acaagcatac tggccaggat aaaagaaagg
     3121 atattcagcc acatcaccgg atgcccgagc tgggcatagg ccaggttctc tgtggttacg
     3181 ccggtcagca ccatggcaat gactacaatc agggacgtta aataattggg gatggcttca
     3241 gtgatccata atataatgga ggccacaaaa atgcccagca tcgcataatt aatccgtgaa
     3301 aaattctcag caccaatctc tttcagccgt ttgagactgt catcccgcaa cacttcagtt
     3361 ttgatctggt tcaggaagga aatgtcaaca aagtaataaa tgagaataaa actgagtatc
     3421 gccagtggcc ctcccagcca ttccatccat ttctctacgc ccttcttctc catctttttc
     3481 agcttctcta cccggtaatt attcatatcc agcgggtcaa aggctttggg gttgtatcgg
     3541 ggatctgttg tattcatgtg gagcaggtta tgtagtaatg tccgttatat ctttagtttt
     3601 accttgaata attttgaact ctggtcgcaa ccgatccagg cggtttggtt ggcgcgatca
     3661 atcaccaggg cttcggcttt cggcacaaaa tcaatttcct gagaggctaa cacgtttccg
     3721 ttcagtgtgc aatgaaaaag taattgctgc tgactgtcgc agatccataa actgttatca
     3781 ctggcgtcaa aaaaaatatc cgacaggtag ctggcaaaat caaccaatat tcgttggact
     3841 tccgcctggc ttgccagcga atatttgatc agcagggtag gagattcctg gttcacgata
     3901 tacagtgtat cctggccgta tgccagacct tcaattcctt tattgctcac tccgcccgga
     3961 atattaatat gggtcaccag gttcagcgag gactgattgg gtgttagccc atataaattc
     4021 attgaagatt catcagcgac aaacaaccgg ttatttaccg catccagggt gatggcttcg
     4081 aaatcatatt gacccgtaac cggtagctgg cggatggttg aaccgtccgt atttatttca
     4141 taaatacttc cctggtcact gatggcatag aaactggatt tagtggctga aaaacaaaga
     4201 ccggagaaac cggatattcc ggtattgtat tcctcatatt ctttatcctg aggtccaaaa
     4261 cccctgttgt ctttccggca gccggaaacc agtaatatac ttaccgctcc tgatatccat
     4321 atggcgctgt tccatttcat agtgacagtt gaataaagca gccggccggt cttttccgga
     4381 atgaccggcc gctttatgga ttacctgaaa caattaccat tttgtaaatg gatttttcat
     4441 gagcatcgag ttataatact tcacatcacc ggtcacttct tcacccagcc aggctggctt
     4501 ttcaaaatga tcattctcgg cctgtaattc cacttccgcc accaccagtc cctggttttc
     4561 accataaaac tcatctactt cgaatgtgtg atggccggct tttacttcat agcggatttt
     4621 gtcgatcacc ccgggttcac agatattcag caattcttcc acttcactta cggggatctc
     4681 tttttcccat tcatacctgg atgcgccgga gtcatttcct tttcccttga tcgtaataaa
     4741 ccctgctttt cccttgatcc gtacccttac tgttctttcc ggcactgatg aaagataacc
     4801 ctgggtgatg cgggtctctt tggccgcttc agttttaaat gcgtcggagg taacaaggaa
     4861 ttttttttct atttcctgag ccatagtagt atttttttta gttagctaat ggttgactga
     4921 tcatgccggt tacgcgtttg atggcctgga gataattgat attctccacc ccgtccagac
     4981 cgatatcggc caccgttttt ttgatgtctt ccacttcagt tgattccgag ttaatggtca
     5041 cgactttagc cccgttgatc agtacattcc ccacttcaca aatggtgtta tttaccatat
     5101 agccaaagcg ttgtttatgt acccttaccg cctgcagatc ggggtgagca ttgatcatgc
     5161 cgataaattc atcataactg taggtttctt tatccagcac cggtgcggtt acctgaaaag
     5221 ccggaaacac ttctttcaaa agtacttctg ccgctatcgg gaaactgcct ttcatcagcg
     5281 gattccattg ctccatcccg tctaccgtct gcacaaaagt ttttatatcc attttgccat
     5341 cccggatctt ggtattgtta atgtcattct tccgggacag aatatagatc tcatcactct
     5401 gacgttccca tactttttca ggtaccggca tagataaccg ggccattcgt tgtgccgcct
     5461 tttcaaaatc ctgtccgaaa gaacggaatt caaaccgggg tttggagatt tctccaatct
     5521 ttaattcttc tttactcatt gtaggctttt ttatttttaa atcaatacag taaaaggctc
     5581 cggggaattc cccggagcgg atttgtttat aaagccggga tatcaccagt ggatgcgcca
     5641 ttcactttcc ccggagtttg cagggccaac ttccaggtac ctgtggcatt gggaacacgg
     5701 ctgtatgagt cggttgcggg aatggcggaa atagtaacac cccagggggc tgcgcctctc
     5761 gtaaataaac tcactgaagt gccatcaggt tttaccagtt ccagtgataa ggtttgtttg
     5821 gcagacatac catcgccaac tgtgccaatg atgagggcag caggatcagc cgttgcggtg
     5881 tatccgctgc atttgatgat gccatagcca tttgccggta aaatagtcgc ggcgggtact
     5941 gaccatggtg tgccgtcggg atttttcagg gagacagtgt ttttaagaat gaccatgccg
     6001 gaaatattaa ccggagcggc tgagttattg aagagttcta caaacttggt attcccgtca
     6061 atttcgttca gtacaatagc ggcataattc ggaggaatgg ctccaacctt gtaggttttt
     6121 tctgcagaac tgttttcaat tccaccggta gtttttgccc ctacatataa aacgatggtg
     6181 gtttggtcaa cctgcttggg gatactgccg gaatacgaat tgctggctcc ggccaccatg
     6241 ggtacagata gggccgtacc gccatttact ttatagaata gggttaccgc ttcaattcct
     6301 tttacagaag taacagtggc ggtgacgttt accacatcgt caggagttac cgtcgacggg
     6361 gtgaaggcca cattgctgat cgtggccggt ccattatata tttcatcctt tacgcaggaa
     6421 gaagcgaata aagccagtcc gatcaggctg cttataaaat aggtaagttt catataagtt
     6481 gttttacagg attattaaaa gttgatttca aaacgcaccc ccatcattcc ataatcttca
     6541 ccggctgttc cattcgcatc ggctactttg gtgggatctt tggtcaatgc acctgcgaga
     6601 tcatatccga cgaataattt cttttttcca ctggcataac ggtcatgatt gacataactg
     6661 taattgatct ggaagcgcac attctttgcg gcataaatat taacaccggc tgtataagct
     6721 tcagcagcac caccaaaaag agcggggtcg ttcatatcaa tattgtcata gcggaaggcc
     6781 agttccatat ctccccattt tttaccccgg tatggctgac tgaattcggc ttctgtatgg
     6841 ttgtaaaact ggcggcctcc gaataaaaga caggcagcct gggcgtagaa tccattaaaa
     6901 ttcagggtgg agagattgtt attccggatt actttacttc tgatatactc gctctggaaa
     6961 ccaaaccctt taaaatagcc ggctatttca atattgttga gtacggtgtg atttacattc
     7021 ggaataatat cagtatccag gtattttttt cggttgatac tgctcaacga gcgggtgctg
     7081 tatcttaatc cgccgtattc ggcggcctct acactggttt tgggctgacg gtaagaagcg
     7141 gcataagcca ggtgtccgcc gaaatctttg tcggctttgg tcggttgcag ggcaaattta
     7201 ccggtgaagg atacgccttc atctcgtcca taatccttat tgttatcttc tacaaaagtt
     7261 ctcatttccg catcttccac ttcctggaag aatacaccac cggctgccag aaaatgggtg
     7321 ccgctatagg aagtttccca acccacatgg cgggaaggag tgaacgcagt caccaccatc
     7381 gccctttcca gaaaattgag gtaacgggag gaagaagtct gggacatcga gaaacgctct
     7441 ttgaaattac ccaccctggt ggccagaccg tttctgaaat catactgaat gtaggcatca
     7501 ttcagttcga gtatcccgtt ggaaaaatca agatcgattt ccccatacca gttcttggtc
     7561 agattggtct tggccgcaaa ccgggcccgg cgaatcgata cgccattacc gatcgggttc
     7621 atcgttttat tggagaatat ctgaccatct acctgcaccc tgatatcaaa ccaaagccgg
     7681 tagtcctggt cttttgattc aaaaactaaa atgccgtttc tgttctccac atccagcgga
     7741 gaacggctga ccagcctgcc gtattggtta aaccggtcct gcctgctggt gtcctgggca
     7801 attgccatat gcatggataa aagcactgcc acgataatca atattcgttt catggtatct
     7861 gttttaggtg ttaaattgtt tttatgaatt gtgtcagatt tatttctttt ttcaggtcaa
     7921 atttttttct taaacggtac ctggctattt caacactttt agttgaaata ttcaggagcg
     7981 gggcaatttc tttactggag aaaccaaccc gtaacagtaa agcgagttta cgttcctggg
     8041 cagacaggtg gggtgatagc tccgtgagcc gggaagggaa ttcgtcattt acatgctcag
     8101 cctgctggta gatctgttca atttcatcgg taaagctcat cttctgtttt acggaccgga
     8161 ttacttcacg gatctgactg ttcttttttt gcggatcaga ttccctgcaa cattcttcaa
     8221 gcgccgcaca ggtgagttca agaaactggc gctggtccgc aatattcagc gcataattaa
     8281 ccagttcttt acgtttttct tccagccggt tatgcagtga gctgttttcc acctgcactt
     8341 gttttatttc tatttcagac agcgattttt gcagttccgc gtattgcaat tgttcattca
     8401 gccttgtatt gtattccctt gctgccgttt tactccggta atgacggaag tacaggtagg
     8461 cggttatgcc cattacggcc aacgcaatag ctgtaacgga gatcagtacg gtattaaccg
     8521 gcagattgat tacggtggtg gttgggccgg cagccgggga agtaaccaca gtcagttgtt
     8581 tatgggtgat atcaattttg aaaacattat ttacaaaaaa gagactgatg aaggtcagca
     8641 agccggccag tacaggcgtc atcaaccagc ccagcatgat cgtgcccagc attttgaacc
     8701 gtatttcctg aatgccttta atggctccaa taccaatcag tgctccaacg attacctggg
     8761 tgctggaaac cggcaccagt ggaatagccg gaaggccgat ccggacaaaa agatcagata
     8821 acgcagttga ggaaaagatg aataacacga gggcctgtga caataccact acgatggcgg
     8881 tctccggcgt catctccagt aatccatggc caacagtatt aattactttt ttactatacg
     8941 tgagaatccc tgccgcaatc gaaaggcctc ccagcaggaa cagcacatgg gtgtttgtga
     9001 aggtgaatgg gcctgcatta atatggaagg aagtggaatt cacaaatacc cccattacat
     9061 tggaaacatt attggctccc aggctgaatg cggcaaaggc ccccacaatc agcagcccgt
     9121 tccggatatg actatccaga ataagcacat gcgttttcgt ccttctcata taataccgca
     9181 tcagcaggta taaaagcccg gagaagatgg ctcccagtaa gggacccgta aaccaggaaa
     9241 cggcgattac agtcagtact ttataatcca ccggattgga agtataaaag caccagccga
     9301 tgatggcacc aacaatcgcc tggctggtgg acacaggtag cttataatag gtcatccagg
     9361 tcacaacaat ggcggaacac agggcaaccg taaatgcacc ccccatggca tcgatagagc
     9421 ccaggctgga gagtgtttca gtggcacctg taccttgcag tacggcgcca agaatgacga
     9481 acaggctcgc gatgatggct gctttcctga acttgatcat ccgggagcca accgctgttc
     9541 caaatacatt ggccgcatca ttggcgccca gagaccagcc caggaataat ccgcttgtta
     9601 taaataaaag gaacagcatg ccattacatt attcgcttga tcgccataat cgccaacagg
     9661 tcagccactt tttcagcagt atccgatatg ttttctatgt gcagggtgaa ataacgcagg
     9721 tgaaattttt cactcagttt cagggagggc atttcacgga atatctttct tctcaccaca
     9781 tccgcataca catccgtttc cttttcaaaa aagaataccc ggtgaatttt ttcgttgacc
     9841 gtagacggct cattgaaata ggacttggcc gccggtataa ctgattcagc cgcagaaacg
     9901 gagatctggg tcagttttac aaaatcctga accagttcct gcggaaccat ggggatctcc
     9961 acgtcaaatt gaaacaggtt ttttttggaa aggtcaatga ggttgtccag ttcttcgagc
    10021 aaccggagaa tatcccctct taactggggc attaatgact gggtataaag cgtgttttca
    10081 attttccggc gaatctggtc tgattcaatt tccaggttgg ccagagattg tagattatta
    10141 ttaaaattct ctgtattctt atagagatag ttttttacgc cttctttaaa aagtagtaca
    10201 ctctggtcta cacaattgaa aaagctgtca atcagcgcta tcgatttatt agccttggta
    10261 aaaaacaaaa tagccccctt tttttagttc tatggtaaaa gtagacttgc tgctatccgt
    10321 ttgatactga tataactcag gtgccggagt aacagggatc agtgtatagg gattaaatca
    10381 ggtaattacc tgatattcac gaaagcgttg aagagatgta gaggtggctg ttattcagtt
    10441 gacttcattt gtaaagaagc tgtataggtg atttagtaaa aacagccttt acaaattggt
    10501 ttaaatcgga catatttctg tcgccccgat caggttttaa accggagtct gtcagatcag
    10561 ttgaaaagaa cagtataatt cataaaagac aagagagtag ctaataagag ccgcgtaagt
    10621 tcttttttcg gcttaatggt gactttctgg tttaaatcgg tcgatccggc tatttcccgg
    10681 taaccatcag ccgggaaaaa ggggatgacc ggacggcgta tatttgtccc aaaatcagcc
    10741 aaatgaacga taaactggta caacgcattg ccgccgaagt ggaagacatt aaatcagccg
    10801 gtctctttaa aacggaacgc attatcgaga gcccccaggg ggccgaaatc attgtcaatg
    10861 gcaaagccgt tctgaatttt tgcgccaata attacctggg actttccagt caccccaaag
    10921 tgatccaggc cgcccatgaa gccatcgata aaagggggta cggtatgagc agtgtccgct
    10981 ttatctgtgg cacccaggat attcataaag aactggaaga aaaaatctcc cggttcctcg
    11041 gcaccgagga tactatatta tatgcggccg cctttgatgc caatgggggg gtgtttgagc
    11101 ctctcttcaa cgaacaggat gccatcattt cggatgccct gaaccatgct tctattattg
    11161 acggggttcg cctatgtaag gcccagcgat tccgttacga gcacaatgac atgaatgacc
    11221 tcgaagtcaa actgaaggaa gcagccggct gccgaagccg tatcatcgtc accgatggct
    11281 ctttcagcat ggatggcacc attgcccgcc tggatgaaat cgtgaaactg gccgaacaat
    11341 acgatgcct
//
LOCUS       DAOPMW010000072        11089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6298_length_11089_cov_9.283397, whole genome shotgun sequence.
ACCESSION   DAOPMW010000072 DAOPMW010000000
VERSION     DAOPMW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_11089_cov_9.283397"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLU11_05515"
     CDS             complement(<1..195)
                     /locus_tag="PLU11_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58605.1"
                     /translation="MKKIFLFQLALLLTAGQYIAAQGTVVDLYSFPDKTAYNYMQVLV
                     FANEGPASAKMVLDQREQIQA"
     gene            complement(223..1152)
                     /locus_tag="PLU11_05520"
     CDS             complement(223..1152)
                     /locus_tag="PLU11_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58606.1"
                     /translation="MEIKFKTPLLFCFIGLLSVHGACQSNNKQNKPKTAANKPAAAPA
                     PKSGGALNAKIGAVYAKEGKPGVFDVDIRVNEGVVKTGDKVDVVSADGKRFSFTVTHM
                     RNPYEDIKKADKEAGTVYIILEGPADAKFDADFALVNPGGGAGAAPAVSTAKFNATIN
                     GKPWKGKDFPYSFSLFKKGVKNMAGNKPYLMLAFKSMDAVDDRQLTLMVFTADPKPGV
                     YTKEKLEVLFSGSPVGDIKKPEMWGYKYPGSVSGALQLEITSYKETGSGKALISGKLS
                     GKFVKILGKAEMTVENGVFTDIPVDVYTEVYLK"
     gene            complement(1189..1833)
                     /locus_tag="PLU11_05525"
     CDS             complement(1189..1833)
                     /locus_tag="PLU11_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58607.1"
                     /translation="MSVLIYSVVGCGDSAAPKEGTKEEKKDTTATENTAPAPAAEVNT
                     EKAVVSFKVNGVQAKTTKGGANDSESQLGMLTAMNNQLGLDLLGDDPGHPHRGWLHIV
                     INDFKMEPGSYTVTGGNMVQFTRYETANAGGSKDYVASREPIDKGTDMTIQFTNVVKN
                     EATEFGEEWLVSGTFSSKMLVKEYSMTKRSSNEGLEITEGKFENVPVKILGKRR"
     gene            complement(1920..3194)
                     /locus_tag="PLU11_05530"
     CDS             complement(1920..3194)
                     /locus_tag="PLU11_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58608.1"
                     /translation="MKRIFFLIAFSFLFAGSVKAQPLSREKDLKPLALKLEPDGSDKE
                     VLVAAAEGSASEKGELILLSGQDMFQPLIIMVYAGNTANPVQVSLHKDSWDDNEMSAS
                     TDASEGYCEFKVRSAGEVGIKLKGNAASYVVMAMAGKEIMPKIESPFVKKGKENKETG
                     KKEEGGKSNTLLYVIIGVLAIALIGVLIKFAGRKNKTISLLLISLSLATLTSAQTRIR
                     GNSVEEIRAEVERILEKNEKEEREKLDKVREHTEKAYKILKGGKDLWEAYKNLGSCMN
                     TAIPAGMPSVPTFCYEADMNESGEGPCAKCFEQARAGFNEVRYKFIKLAIIYRCTKSF
                     ADKSMAFGDDVSSIHGVSGLAWQAEKRKIEESVERLKKSYDDKYIELLQRLQESMIDI
                     AECEEEFGERDWYDRFGFMFYEFMKEKYRRTD"
     gene            complement(3218..4516)
                     /locus_tag="PLU11_05535"
     CDS             complement(3218..4516)
                     /locus_tag="PLU11_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN58609.1"
                     /translation="MRLALIFFYLIVCIQPVSAQLDWKQPTVYTYNGKEAEILYRYGD
                     VDNMNMGWSPGYNPFSGNNTLPHIFPFEPAPSDPAGTDRIMVCSGYKAGNRNTDGYTA
                     VTERPVNNPVPLFLKWDKKDVLVKKVVMQLFVDDFQPTVFGKNYQVFFDGERIPYLEY
                     VINSLSQSGPIGKLITIDILPQYLPFFEDGELSLIIDDPYTDSGDGFAIDFVQLLINP
                     KNTSTSIVKGKVLHQQTRKPIAGALVTASNGAQVYTGKDGQFRLGGMTPGLAVISAMK
                     TGFRQSFVNTDVNVKTASIVIILELYPFANEDAGSMQRILKEKGQLDLYGIRFDANSD
                     KIRPESASLLSELLALLQQNPKLKLEFSGHTDADGDEAANLSLSTKRAEAVLAWLKNR
                     NAPVNNLVARGYGESKPIASNKTAEGKELNRRVEIKVLSQ"
     gene            complement(4617..5363)
                     /locus_tag="PLU11_05540"
     CDS             complement(4617..5363)
                     /locus_tag="PLU11_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN58610.1"
                     /translation="MIQALIIDDEKNAIEVLELQLSRYCKQVQVLATAQGGVQGIELI
                     RKYQPDLVFLDIEMPHKNGFDVLNETKDCRYKVIFTTAYDQFAIRAFKFSAVDYLLKP
                     IDIRELQEAVEKAAIKEEQGSLDEKLQVLISQLQAPVRQERIALPVGDGMQFFHTDEI
                     LRCESDSNYTHISLTNGKKITIAKTLKEVEESLRGVDFYRIHQSHLVNMNHISKVVKG
                     EGGYVVMKDGTTITISRNKKEAFLESFRKL"
     gene            complement(5374..8481)
                     /locus_tag="PLU11_05545"
     CDS             complement(5374..8481)
                     /locus_tag="PLU11_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN58611.1"
                     /translation="MSRALFIWLIFLFSIPLLGQDPANNRFVNFGSKDGLPEKYIYSI
                     TQDKKGYIWTGTGTGLFRYDGISFKPFRSPLDKTGANISNILQAVYTDPRGYLWLGSL
                     NDLQCYDPATNHFWRPAQQDTLIRKLGSYYINRFTPSADGGLFIQTANRFFYRFNPRD
                     SSIRDYSSLFPVTASRYTLKVIEYKGTVFAVHAEGIYRFTQSGQEAVFYPFPYKGDEI
                     TNAAIASEGILLTSYTHGLLVFLPDNGQYKESRYNNPKLRLNNLFTVLEQNNELWLGS
                     YPLFRMKEGRLTTWENQRLNEHEIMANKIGDLFFDREGNLWIGSHNGISMMSWQNQQI
                     ETVQFKDPVTGTQVEPTGVFNLPGSNDLLVPNTSSTGLIYYSSRDKKISVIPNPLVKE
                     KGSSRIIGLILLNDGSVIASDDQHLFRFDPLNRKLIPFELKDQDGKPVINVGRNLIDR
                     RGHVYISSHNNGFYIWKPAENRVFHYNKTVVDPLADLEKDNQLYPCLADSRDHIWFTS
                     SNGVYEYLPGQQKWNHYNAEGMDDLPAMTQTTYIAEDRQGHIWVTTQNNGLYEIYTER
                     GKKQIRNFGKNSGIGLSTDYCIKLKADKQQPYLWLTTINGLHRFDPVARRIISTLSIQ
                     HGLAKDGGSYSYNITDSNQLIQMYYGAASFINLNNYRFNTQAPKVELTSVSVMDKEVL
                     YIAISDKPIELDHNQNFLQFEFSALQFNNHNQLQYAYLLEGADKDWIYSGNRNFVSYS
                     GLGPGKYVFRVKAANNDGVWSNQETRIELMIREPIWRRDWFLVLCAGLLMLGMWLIYR
                     FRIKRIRREESLKAGFHQQLADMEMRALRAQMNPHFIFNSLNSIQKYILKNDQYAASQ
                     YLTKFSRLIRLILDHSNQNNILLSSETELLQLYCEMESLRFDHRFNYRIQVDASLQPD
                     TQEIPSMLIQPYVENAIWHGLLHKETTGNLLIEFKKTADGQLIVMVEDDGVGRSRAAE
                     LKSKQVLKKKSYGMQITEDRIALINRLNKIQASCRVLDLTDAQGRPAGTRVELVIPLK
                     QPDQS"
     gene            complement(8678..9973)
                     /locus_tag="PLU11_05550"
     CDS             complement(8678..9973)
                     /locus_tag="PLU11_05550"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPN58612.1"
                     /translation="MKTCQYTIAGIVLLLGLNFQSQAQKVTKVKSDWHLGFNSGFYVY
                     QGDLTPSVAGSWKTVKPGVAFSLSKTLNQHLSLQYALSLATLEGDDMKYKSSQYYRGL
                     RRFYFRSTIAELTVSHQFYFRHQFNRELRFQPYLSAGAGVGFLLNSQRNSSQTVFSYF
                     AADNLQNKVAEDSTYGMPSIILAVPVGLGFNYRLNERLLFNLEGLYRFTSSDYLDGFS
                     RAGNNKKNDYYYGLSVGIRVLLGKKSGGKHAAPVLVPVPQPAPLLKLKNEEAPVFPDK
                     DLDGIADSIDRCPQEKGTMEFYGCPAIPARQSVNAPAAVTTTAPVFPGFTIYFAYDRS
                     SLDGAGFANLNEVIRLLKADPALIVVLKGHTDMKGSLESNYKLSLSRSKVCADYLASY
                     GIDRNRIKLEAYSKLQPAADPAEERLQWKNRRVEVVLVN"
     gene            10369..10725
                     /locus_tag="PLU11_05555"
     CDS             10369..10725
                     /locus_tag="PLU11_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58613.1"
                     /translation="MKSLLSILLTFTLSFLTVSCTKSGDDGLACETNETTRVTFKNTT
                     SVSQRVEVAKTFNAQFVPLDAVLSFDLAPGASSVKEFSDGRYFIQWKSNCSSTCAQSA
                     FYAKEFLMCGDTEEAL"
     gene            10830..>11089
                     /locus_tag="PLU11_05560"
     CDS             10830..>11089
                     /locus_tag="PLU11_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58614.1"
                     /translation="MFIRAIRGLPFCIFRALSPHLAGQACILVANPFFAAGKSIRAYP
                     RYPWPAFLYFSCPFVTFRGYPFLPQANQSMANPFAAGKTTLI"
BASE COUNT         2824 a         2743 c         2337 g         3185 t
ORIGIN      
        1 tgcttgtatt tgttcgcgct gatcaagtac cattttagcg gaagcgggtc cctcattggc
       61 aaatacaagt acctgcatat aattataggc cgttttgtct ggaaaggaat aaagatcaac
      121 aacggtaccc tgtgcagcta tgtactgacc ggcagtaagg agtaacgcta actggaaaag
      181 aaaaattttt ttcatagtac tgtttaaaga acggataatc gtttacttca gataaacttc
      241 tgtatacaca tccacgggaa tatcagtaaa taccccattc tcgaccgtca tctctgcttt
      301 acccaggatc tttacaaact ttccgctgag ttttccggag atcagggctt tgccgctacc
      361 cgtttcctta tacgaagtga tctccagctg cagcgcaccg gatactgaac cgggatattt
      421 gtaaccccac atttccggtt ttttaatatc tcccaccggt gaaccggaaa ataatacctc
      481 cagcttttct tttgtataca cgccaggttt gggatcggca gtgaagacca tcagggtcag
      541 ctgccggtca tccaccgcgt ccatactttt aaatgccagc atgagataag gtttgtttcc
      601 cgccatattc tttactcctt ttttaaacag ggaaaaagaa tagggaaaat ctttcccctt
      661 ccagggtttg ccgttgatcg tagcgttgaa ctttgcggta cttacggctg gtgcggcccc
      721 tgcacctcca cccggattca ccagggcgaa atccgcatca aacttcgcat cagccggtcc
      781 ctccagtata atataaacgg tgccggcttc cttgtcagcc tttttaatat cctcatacgg
      841 attgcgcata tgcgtaacag tgaatgaaaa ccgtttaccg tcggcagata ccacatccac
      901 tttatcaccg gttttcacca ccccttcatt cacgcgtata tccacatcaa aaacccccgg
      961 tttcccttcc ttggcataga cggcaccgat cttcgcattc agcgcacctc cggatttagg
     1021 agccggagcc gcagcgggtt tattggccgc tgtttttggt ttgttctgct tgttgttcga
     1081 ctgacaggct ccatgtaccg aaaggagacc gataaaacag aataaaagcg gcgttttgaa
     1141 tttaatttcc ataaataacg aataatggtt atggtttttg atctgagttt atctgcgctt
     1201 accgaggatc ttcacgggta cattttcaaa ctttccttcc gtgatctcca ggccttcatt
     1261 ggaactgcgc tttgtcatgg aatattcttt caccagcatt ttcgaggaaa aggttccact
     1321 caccagccac tcttctccaa actccgtagc ttcattcttc accacattgg taaactgaat
     1381 ggtcatgtct gttcctttat caatgggttc cctgcttgcc acataatctt ttgatccgcc
     1441 ggcattagct gtttcgtagc gggtaaactg caccatattc cctccggtaa cggtatagga
     1501 acctggttcc attttgaaat cgttaatgac gatgtgcagc cagccccggt gcggatggcc
     1561 gggatcatcc cccaacaggt caaggcccag ctgattattc atagcggtga gcatacccaa
     1621 ctgtgattca ctgtcattag cacccccctt tgtggttttt gcctgcactc catttacttt
     1681 aaaggaaaca actgcttttt cggtattcac ttctgcagca ggcgcaggcg ccgtattctc
     1741 cgtcgccgta gtatcctttt tttcttcctt tgtaccctcc ttgggtgccg cactatcccc
     1801 acaacccact actgaataaa tcaaaaccga catgatgaaa aaatttctga taagcattct
     1861 ttaagatttt aaatattaat gaatcgcaca cttataactt ataacttatt acttaccact
     1921 caatccgtcc tcctgtattt ctccttcata aattcgtaga acataaatcc aaaccggtcg
     1981 taccaatcgc gctccccaaa ttcttcttca cattccgcga tatcgatcat ggattcctgt
     2041 aagcgctgca acaattcgat atacttatca tcatatgatt tcttcagccg ctccaccgat
     2101 tcttcgatct ttcttttctc cgcctgccag gccaatcctg ataccccgtg tatcgaggat
     2161 acatcatccc cgaaagccat ggatttatca gcaaaggatt tggtacaacg gtagatgatg
     2221 gccagcttga tgaacttgta acgcacttca ttgaacccgg ccctggcctg ttcaaaacat
     2281 ttggcacagg gaccttcgcc ggattcattc atatcagctt cataacaaaa agtaggtacc
     2341 gaaggcatgc ccgccgggat agcggtattc atacaggagc ccaggttttt ataagcctcc
     2401 cataaatcct ttcccccttt cagtatctta tacgcttttt ccgtgtgctc ccttacttta
     2461 tcgagttttt cccgttcttc tttctcattc ttttccagta tgcgttccac ttccgcacga
     2521 atctcctcta ccgaatttcc cctgatacgg gtttgtgccg aggtgagcgt agccaggctc
     2581 agactaatca ataacaggct gatggtttta tttttccggc cggcaaactt tatcagcacc
     2641 ccgatcagag cgatggcaag caccccaatg atcacatata aaagggtatt ggatttgcca
     2701 ccttcctcct ttttacctgt ctctttattt tcctttcctt ttttaacaaa gggggattca
     2761 atcttgggca taatttcttt tcccgccata gccatcacca cataggaagc tgcattgcct
     2821 ttgagcttaa taccaacttc accggcagac cggaccttga attcacaata gccttccgac
     2881 gcatcagtag atgcactcat ttcgttatca tcccagctat ccttatgcag acttacctgc
     2941 accggattag ctgtattgcc ggcatatacc ataataatga ggggctggaa catatcctgt
     3001 cctgaaagaa ggatcagctc tcctttctcc gatgccgatc cttcggcagc cgccacgagc
     3061 acttccttat cgctgccatc cggttcgagt ttaagtgcca ggggtttcag gtctttttcc
     3121 cgggataaag gttgtgcctt cacgctgcct gcgaaaagaa aactgaaagc aatcagaaaa
     3181 aatatacgtt tcatgaatag cggtttatga attaaggtta ttgacttagt acttttattt
     3241 ccacccgccg gttcagttct tttccttcag cggttttgtt gctggcgatg ggcttgcttt
     3301 ctccatagcc tcttgccaca aggttattta caggagcgtt tctgtttttt aaccaggcca
     3361 ataccgcttc tgcccgtttc gttgaaaggc tgaggttggc ggcttcatcc ccatctgcat
     3421 ctgtatgccc gctgaattcc agtttcaact taggattttg ctgaagcaat gcgagtaatt
     3481 cactgagcag agatgcagat tccggcctta tcttatcact gtttgcatca aacctgattc
     3541 cataaagatc cagctggcct ttttccttca ggatacgttg catgctgccg gcatcttcat
     3601 ttgcaaaagg atacagttca aggataatta caatggatgc cgtttttaca ttcacatctg
     3661 tgttgacaaa agattgacgg aacccggtct tcatggcact gatcactgcc agtccggggg
     3721 tcatcccacc gagtctgaac tgaccatctt taccggtata gacctgtgcg ccgttggaag
     3781 cagtaaccag tgcgccggca attggtttac gggtctgttg atgcagtacc ttccctttta
     3841 caatgctggt gctggtgttt ttggggttaa tcagcaattg tacaaaatca attgcgaacc
     3901 catcccctga atcagtataa gggtcatcta tgatcaatga gagttcgcca tcttcaaaaa
     3961 aaggcaggta ttggggtaat atgtcaatgg tgatcagttt accaatgggg cccgattgcg
     4021 aaagtgaatt gatcacatat tccagatacg gaatccgttc gccatcaaaa aagacctggt
     4081 aatttttacc gaataccgtt ggctgaaaat catccacaaa caactgcatc accacctttt
     4141 tcaccaacac gtctttttta tcccatttca agaaaagggg aaccgggtta tttaccggtc
     4201 gctcagtgac cgccgtatag ccatcagtat tccggttgcc ggctttgtac ccggaacaaa
     4261 ccatgatacg gtctgtacct gccggatctg aaggtgccgg ctcaaacgga aatatatgcg
     4321 gcaatgtgtt attgcctgag aagggattat atcccggaga ccaacccata ttcatattat
     4381 ccacatcacc ataacggtaa aggatttcgg cttcttttcc attataggta tatacggttg
     4441 gttgcttcca gtcaagctgt gcggataccg gttgaataca gacaatcagg taaaaaaaaa
     4501 taagggccag gcgcatggtg tggatttgac gccttgaaat tgccaagaat tccggttagc
     4561 cggaacagtg ctttagtatc ccgcggtttt tattgagtat ttgcagttat ggctgatcag
     4621 agtttacgga aactttccag aaatgcttcc tttttattac ggctgatagt aatagtggtg
     4681 ccatctttca taaccacata acctccttcg cccttcacta ctttactgat atggttcata
     4741 ttcaccaggt gactctgatg aatccggtaa aaatcaactc cccgcagact ctcttccact
     4801 tctttaaggg ttttggcaat ggttattttt tttccattgg tcaatgaaat atgagtgtag
     4861 ttgctgtcac tttcacaacg caggatctca tctgtatgaa agaactgcat gccatcacca
     4921 accggcaggg caatgcgttc ctgccggaca ggagcctgta actgactgat cagcacctgg
     4981 agcttttcgt ccagtgagcc ctgctcctcc tttatcgcgg ccttttccac ggcctcctgc
     5041 agttcacgga tatcaatagg tttcagcaaa taatccacgg cagaaaattt aaaagcccgg
     5101 atcgcaaact gatcatatgc ggtagtgaaa atcactttat accggcaatc ctttgtttcg
     5161 ttcagcacat caaacccgtt tttatgtggc atttctatat ccaggaaaac gagatctggc
     5221 tggtatttgc ggatcagttc aattccctgt actccacctt gtgcagtggc gagcacctga
     5281 acctgcttac aataccggga caattgaagt tcaagtactt caatcgcatt tttctcgtca
     5341 tcgataatta acgcttgaat catttctctt tttttaagat tgatctggtt gtttcaacgg
     5401 gatcaccagc tccaccctcg ttccagccgg ccggccctgt gcatcggtca ggtccagtac
     5461 ccggcagctg gcttgtattt tattcaaccg gttgatcagg gctatccggt cttccgtgat
     5521 ctgcattcca tagctcttct tcttcaatac ctgtttactt ttcagttctg ctgccctgga
     5581 cctgcctacc ccatcatctt ccaccattac gattaactgg ccgtctgctg tctttttaaa
     5641 ttcgatcagc aggttacctg tagtttcttt atgcaacaag ccatgccaaa tcgcattctc
     5701 cacatagggc tgtatcagca tcgaaggaat ttcctgggta tcgggttgca acgaggcgtc
     5761 tacctgaatc cggtaattga accggtggtc aaagcggagg ctttccattt cacaatagag
     5821 ttgcagcaac tccgtttcac tgctgagcag tatattattc tggttgctat gatcaagaat
     5881 caggcggatc agtcgtgaaa atttggtcag gtattgtgaa gcggcatact gatcattctt
     5941 taaaatatat ttctggatac tgttgagtga attaaagata aaatgaggat tcatctgtgc
     6001 ccgtaaagcc cgcatttcca tatccgcaag ctgttgatgg aaaccggctt tcaggctttc
     6061 ttcccgtcgg atccttttta tccggaatcg gtaaatcaac cacatgccca gcatcaataa
     6121 accggcacaa agaaccagaa accagtctct tctccagatc ggttcccgta tcatgagctc
     6181 tatccgggtt tcctgattac tccatacccc gtcattattt gccgctttga cccggaagac
     6241 atacttaccc ggccccaacc ccgaatagga tacaaagttc cggttaccac tatagatcca
     6301 atctttatcc gccccctcca gcagataggc atattgtaac tggttatggt tattgaactg
     6361 cagagcagaa aattcaaact gcagaaaatt ctgattatga tccagctcaa taggcttatc
     6421 ggaaatagct atataaagta cttctttatc catcacgctt accgatgtaa gctccacctt
     6481 tggtgcctgg gtattaaaac ggtaattatt caggttgata aaacttgcgg ccccgtaata
     6541 catttgtata agctgattac tatctgtaat attatacgaa taactacccc cgtctttcgc
     6601 caggccatgt tgtatggaaa gcgtactgat gatccgccgg gcaaccgggt caaaacgatg
     6661 taatccattt atagtggtca gccataagta cggctgctgt ttatccgctt ttagcttgat
     6721 acaataatcc gtgctgagtc cgatccctga attcttgccg aaattccgga tctgcttttt
     6781 tccacgttca gtatagattt cgtacaaccc gttgttttga gtggtcaccc agatatgtcc
     6841 ctgacggtcc tcggcgatat aggtggtctg agtcatagca ggaagatcat ccataccttc
     6901 cgcattatag tgattccatt tctgctgccc cggcaggtat tcgtatacac cattggaaga
     6961 cgtgaaccat atatggtccc ggctatcagc aagacaggga tagagctgat tatctttttc
     7021 cagatcggcc agcggatcca ccactgtttt attataatgg aacacccggt tctctgccgg
     7081 tttccagata taaaacccgt tattatgtga gctgatatag acatgccccc tccggtcaat
     7141 caggttacgg cccacattaa tcaccggctt gccgtcctga tcctttagtt caaaagggat
     7201 cagtttccgg ttcaacgggt caaacctgaa taaatgctga tcatcagaag caataacgga
     7261 accgtcgttc agtagaatca atccaatgat ccggctgctc cctttctctt tcaccaatgg
     7321 attaggaatc acactgatct ttttatccct tgaggaataa taaatcaatc cggttgatga
     7381 agtattggga accagcaggt cattggagcc tggcaggttg aaaacaccag taggttccac
     7441 ctgggtaccg gtaacgggat ccttaaactg aactgtttca atctgctggt tttgccagga
     7501 catcatactg attccgttgt ggctcccgat ccagagattc ccttcccggt caaaaaacag
     7561 atccccgatc ttattggcca tgatttcatg ctcgttcagc cgctgatttt cccaggtcgt
     7621 cagccggccc tctttcatcc ggaagagcgg gtaagagccc agccatagtt cattattctg
     7681 ctccaatacg gtaaacagat tattgagtct aagcttcgga ttattatacc gtgattcttt
     7741 gtactgtccg ttgtcgggca ggaatacaag caatccatgt gtatagctgg tcagcagtat
     7801 cccttctgaa gcaatggcgg cattggtgat ttcatctccc ttataaggga aagggtaaaa
     7861 aacggcttcc tgcccggatt gcgtgaagcg gtagattcct tcggcatgta cggcgaatac
     7921 agttccttta tattcaataa cttttaaagt ataacgggat gctgtaaccg gaaataatga
     7981 agaataatca cggatactag aatcccgggg attaaaccgg taaaaaaaac ggttggccgt
     8041 ctggataaaa agtcccccgt ctgcagaagg tgtaaaccgg ttgatataat aactgcccag
     8101 tttacggatc agggtatcct gctgtgccgg ccgccagaaa tgattggttg ccggatcata
     8161 acattgcagg tcgttgagac ttcccagcca gagatatccg cgtggatctg tatacacagc
     8221 ctgcaggata ttggaaatat tagcaccggt tttatcaagc ggactccgga aaggtttaaa
     8281 actaatgcca tcataacgga ataaaccggt gccggtaccg gtccagatat accccttttt
     8341 atcctgggtg atgctgtaaa tatatttctc cggcaatccg tccttgctgc cgaaattcac
     8401 aaaccggtta ttggcaggat cctgacccag caaggggatt gaaaataaaa aaatcaacca
     8461 tataaaaagc gcccgggaca tgttttactg taagtggtct aataacggaa tattccatgt
     8521 taaacttagg cgtttttagt attcggcgga tctgttgatt aaacgggaga aaagaagaaa
     8581 gaacgggcag ggacggaaaa caaaaatctc ctgtccgaag ttcagacagg agatacggta
     8641 agtcaattat agaagtatag ttccgtatgt tgccggatca attcaccagc accacttcta
     8701 ccctccggtt cttccactgt aatctttctt cggcgggatc tgcagcaggc tgcagtttgc
     8761 tgtaagcctc cagtttaatc cggttccggt caatcccgta agaggccaga tagtccgcac
     8821 agacctttga gcgactgagt gacaatttat aattagactc gaggcttccc ttcatatctg
     8881 tatgcccctt caacactaca atcaaagcag gatcagcttt gagtaaccgg atcacttcgt
     8941 tcagattggc aaaaccggcc ccatccagac tggacctgtc ataggcaaaa taaatggtaa
     9001 agccggggaa tacaggagct gttgtggtaa ctgcggcagg cgcatttaca gactgtcggg
     9061 ccggaatggc cggacatcca taaaactcca tcgtgccctt ctcctgcgga caccggtcaa
     9121 tactgtcggc aatgccatcc aggtccttat ccgggaaaac aggcgcttcc tcatttttta
     9181 gctttaacaa aggtgccggt tggggtacag gaacgagcac cggtgctgca tgcttgccac
     9241 ctgatttttt tccaagtaaa acgcgtatcc cgacagaaag cccgtaataa taatcattct
     9301 ttttattgtt acctgcacga ctgaacccat ccaggtaatc ggaagaagta aaacgataga
     9361 gtccttccag attaaatagt aagcgttcat tcagccggta attaaaaccc agtccaacag
     9421 gtacagccag gattattgat ggcatcccat aagtggaatc ctccgcaact ttattttgca
     9481 ggttatcggc cgcaaaataa ctaaagaccg tctgactgct gttgcgctga ctattgagta
     9541 aaaaacctac ccccgctcct gccgataaat aaggctgaaa acgaagttca cggttaaact
     9601 gatggcggaa ataaaactga tgggagacag ttaattccgc aatcgtactg cggaaataaa
     9661 aacggcgcag cccacggtag tactgactgg acttgtattt catatcatct ccttcaaggg
     9721 tagccaggga taaggcatat tgaagtgata aatgttgatt taatgttttt gacaggctaa
     9781 atgccacccc tggtttcact gttttccagg aaccggccac agacggggtc aggtcaccct
     9841 ggtatacata gaagccactg ttgaaaccca ggtgccagtc gctttttact ttagttactt
     9901 tttgggcctg tgactgaaaa ttgagtccca gtaataaaac aattcccgca atggtatatt
     9961 gacatgtttt catctgctga tcggccgttc atgaaaagga gggattatta aattggtatt
    10021 tcgcagaatg aatctttgcc aggactttgc cggtaaggca aaggggatgc aatactaccc
    10081 tgcaaatggc aggatttata atgtttcgtt cttccttctt tgaatacaag tatagttact
    10141 taggccggcc tggcagtaaa ataaacagat accagaccct ggccggtggc atgagggcaa
    10201 accgattact gacaggtttt ccgttaaatc caatcagacc agttgacaga tagctgttta
    10261 aaagcattgt ataaagccag ctgatgacct tttctgcgtc gtccgatgta tagctggtac
    10321 cggttcattc aaccggttaa aattgtaatc atgcttaaaa cagaaccaat gaaatcactc
    10381 ctgtctatcc tgctgacatt tacgctgtca ttccttactg taagttgtac taaatccggc
    10441 gacgatggtc tcgcctgcga aaccaacgaa accacccgtg ttacttttaa aaacacgact
    10501 tccgtctccc agcgtgtgga agtggctaag accttcaatg cccagtttgt accattggac
    10561 gccgtcttgt cctttgatct ggcccctggt gccagcagtg tgaaggaatt cagtgatggc
    10621 cgctatttta ttcaatggaa aagcaattgt tcctctacct gtgcgcagtc agccttttat
    10681 gcaaaagaat ttttgatgtg cggagatacg gaggaagcct tgtaagttag tcgggagttg
    10741 gtagttagta gttagtagtc aacagcccct tctgaggaat atttcgtgga cattcgtagc
    10801 gaacccattt gccgaaggca aatcaatccg tgtttatccg cgctatccgt ggcctgcctt
    10861 tctgtatttt tcgtgccctt tccccgcatt tggcgggaca ggcgtgtatt ttagtggcca
    10921 atccattttt tgccgcaggc aaatcaatcc gtgcatatcc gcgctatccg tggcctgcct
    10981 ttctgtattt ttcgtgccct ttcgtgacct ttcgtggcta cccttttttg ccgcaggcaa
    11041 atcaatccat ggccaaccct tttgccgcag gcaaaaccac cctaattca
//
LOCUS       DAOPMW010000073        10970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6401_length_10970_cov_7.689601, whole genome shotgun sequence.
ACCESSION   DAOPMW010000073 DAOPMW010000000
VERSION     DAOPMW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_10970_cov_7.689601"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PLU11_05565"
     CDS             complement(<1..1496)
                     /locus_tag="PLU11_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58615.1"
                     /translation="MHIRTGILMLIFPALLAANGVYAQSGAADSIRNLLQGGTEDSIK
                     VFRYYQYGELYEQSKPDSADFYFRKGLELAKKLNYQKGIFSYTSYAIVLLNNKGKYLE
                     GLALTQESVEQATQFGNIRDQATAYINVANEWQYLGDLETAAAFYLKAAEQASGLPEK
                     KFERVALNNLASVFIELKDWDKVIAYASKALFIARQMKDDYSIASCLINLAVGKTNKG
                     QIEEAIALYNQVFTIGQQTEDYILRLDGLNGLGEVFLARKDLKTSVRYFHQTLKLAGE
                     NEDPVYAMYANGGLASSFSGIGQRDSAVYYVLQAIRIARENNASLELKNYCLEASGLY
                     EQMGNFKAALDYQKQFQLLSDSLLGEKSNSAILLAEAKFQGKQKEQQLLLQQSSIRQK
                     SMLNYVFIGSTIALLLISLLTYRNYRQKRKLSDQRIADLEREKQLSATQSLLRGQEEE
                     RNRMARDLHDGLGGLLSGVKLQLGAMKGNLVLSEEMGRTFNNALNKLD"
     gene            1647..3005
                     /locus_tag="PLU11_05570"
     CDS             1647..3005
                     /locus_tag="PLU11_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58616.1"
                     /translation="MKPKNTFLISLFLLTGLSMEGQLKNYDIFSYQAPKGFSLQKLDA
                     ALFYSKKDSKSYCQLFLYPATASVGTPDADFDTNWNSFARNSGQGVNDPETKETDTAN
                     GWHIIMGAAKGRFGNKSYVLTLTTLSNGEISFYTASVFSDQKYLPAVQDFTNSIQPDL
                     QLYTSKKTTTEQSSTTQNQSASHTSGKSITKSTTRFDDGWMATPNDNYVQLVKENTEV
                     RLMYINNALDQARPNTADAADYYWNEYVSPYFQIAQVEKWSGVEYPVIYFMQGSGINL
                     STGKPCHVALKIVYSGGARPILVIASDQPQFRKQYPHPNDLDRMLNYNKFALSAADLT
                     GKWTGGSGAGVEYYNAYNGTYAGMSAISTSDDFHFNADGTYTSAYRSASSSQGSTRFG
                     AIDYKGKYQVTDWAIRAENRYKGKTTQFNAQFIAVKNGYLLYLSDSENPAMNYTLIKI
                     AL"
     gene            3002..4819
                     /locus_tag="PLU11_05575"
     CDS             3002..4819
                     /locus_tag="PLU11_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58617.1"
                     /translation="MKITFLALLLCSCIMTMAQVNCATLEINKVPGKWVWQRAGYGAQ
                     WQYGDPLRKEMQRIMPVALDGLHATSSIAFGDQATFWYTKSPAAYEYYLMLKKYECLK
                     GYNTLQQEGETSCWVHFSVNQLDGEKFPMPEQGTGVIFNESRIRVTNIEVQTDAAGNK
                     VIYSNYRPDETIKHCYFFSSRKNLPWRKLNNKELFTAYKAHHETRVIKEIARFEKLVS
                     DDEKKFNSLSSAKKQQQTYWPEIIRKNKEILQRYKDEHKKIITWYTAALKQPDLQSTA
                     YVISINESHFYPDKLTASPGHGYYAWVDNLDFFDKTKPKDEPQCIALYPRKQDTDIPK
                     KNFMELFYSQFNLDVLGRMVGEPAKKPGGINSLNASLNSAKAGSAINQTTINNYRYSF
                     DQSEINRFPQGWNGVKNSQVKPFENNNWLALTRDGYWYPQQYNKDIKDRFSLSFDLAW
                     NKDIAYNSGSFTVTLAEVDYDNTGERYRLDDNQNMYWSLYDSYVGKFNRVMLWFDPYW
                     NGGGLLTVYSYDKTENIKVNKRITLPDFYLTKNVHQLKVQRAGQSLIITINNKKEAEI
                     PDVFLPSVKYNLYTFSRYKGDNSDNKNDVFYLKNIVVDY"
     gene            4915..5847
                     /gene="fmt"
                     /locus_tag="PLU11_05580"
     CDS             4915..5847
                     /gene="fmt"
                     /locus_tag="PLU11_05580"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPN58618.1"
                     /translation="MDQLNRLRIVFMGTPEFAVASLDALVRAGCQVVGVVTAPDKPAG
                     RGMQLQQSAVKKYAVDKSIPVLQPLKLKDPEFINELTALNADLQIVVAFRMLPEIVWN
                     MPPMGTVNLHGSLLPQYRGAAPINWAVINGEKETGVTTFKLKHEIDTGDILLQESFPI
                     TEQDTAGTIHDRMKEIGASLLVKTVTGLAAGTLTEKPQTGNPDMLKHAPKIHTDTCRI
                     NWSLTAGEIYNRVRGLSPHPAAFTILNEKMLKIFRCEKEVVSPKSSAGEYETDGKTFL
                     KIACTDGYIHLLDIQLEGKKKMEITEFLRGYRFA"
     gene            5859..6662
                     /locus_tag="PLU11_05585"
     CDS             5859..6662
                     /locus_tag="PLU11_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN58619.1"
                     /translation="MNIRNLVLLMALTSCLTVSAQKNKKWKSLFNGRNLKGWTAKITG
                     YSLGNNFGNTFRVKDGKLVVSYDQYEGFNNRFGHLFYKKPFTHYLLAIEYRFTGTQVS
                     GGPSWAYKNSGVMLHCQAPSSMTTNQDFPISLENQFLEGDSTGVRPTCNLCTPGTEVM
                     INGKKPSEHCIKSSSPTYRNGEWIRAEMLVLGDSVIKHIVNGDTVLVYHQPSIGGGVV
                     NNFDPSAKPDGKPLTGGYIALQSESSPIEFRKVEIIDLSKKYKRKKISK"
     gene            complement(6652..7656)
                     /locus_tag="PLU11_05590"
     CDS             complement(6652..7656)
                     /locus_tag="PLU11_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPN58620.1"
                     /translation="MKNGILIVLVLLFSSILLQAQKADLYVKSGEKELCLEHKVAPKE
                     SFFSIGRLYNVHPRHIASYNKLDINKGLQIDQRLRIPLTDTNFTQKGNSGTPVYYKPS
                     AVIALADISRAHNNVPATSLKSWNGLSGDQAKKDNKLIIGFLLSKEMPSVTIKAKPVP
                     ATEDKVENKPLPPVLVEEKKEPAKPVVEPAPQPETRQPEVIKQPEIRQPEKKEEKPVA
                     QPVSSSGDNAQGYFKVSFNQQVKKAPLSKEETVTSGIFKTASGWQDQKYYMLIDGVAP
                     GTIIRISNPSNTRVIFAKVLGEMNGIRQNEGYTIRISNAAASALAITDTEKFIVKITY
                     "
     gene            complement(7706..9073)
                     /locus_tag="PLU11_05595"
     CDS             complement(7706..9073)
                     /locus_tag="PLU11_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain protein"
                     /protein_id="HPN58621.1"
                     /translation="MTLPAAFLKSLQGIIGFSPEAFEQVHQSGKQVTSVRLNPAKTDG
                     VLPPEFVSLAEAIPWTGLGYYLRQRPSFTFDPLFHAGNYYVQEASSMFLEQALLQLTQ
                     RAEPVKVLDLCASPGGKSTHLQSLLPAGSLLVSNDVIRSRAAVLRDNIVKWGAEQVVV
                     TNNDPKDFAGLESFFDVLVVDAPCSGSGLFRRDPEAIGEWSLNNVQLCSQRQQRILAD
                     CWPALKENGLLIYSTCSYSREEDEEIMDWIKQELDAENCPLTIKEEWGIVETESPGGS
                     LGYRFWPDRINGEGFFLACFRKRNGDEPGKMNSKKQPERVTRAEKEWVARWMNIAGKE
                     LIKYQQTVYAWPEDHFQDFSILLEKMRVIYSGVKSGELMRDKMVPDHSLAMSGLVLAD
                     VPGIDLVAEDAIRYLQRKEIRLEGVPTGWSRACFAGKSLGWMNILPGRINNYYPKELR
                     ILKDI"
     gene            complement(9131..10546)
                     /locus_tag="PLU11_05600"
     CDS             complement(9131..10546)
                     /locus_tag="PLU11_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPN58622.1"
                     /translation="MELSILTFCYLIASVTFILGLKMLSNPATARKGNLVAAAGMTIA
                     IIGTIFLYEEEGVKLGNYGWIFGGIAIGSIVGTLAAKKVKMTAMPEMVSMFNGMGGAC
                     AALISIVEFNHLVHAADGDLSAHIPTLLIILLGLIIGSVSFAGSMIAWGKLNGKIKDI
                     VFPGQHLLNIGLLLVILALAVYIIGWTPANPSVIFYAIFAISILYGVFFVMPIGGADM
                     PVVISLLNSFTGVAAACGGFLYDNKVMLTGGILVGAAGTLLTILMCKAMNRSLTNVLI
                     GSFGGGHKEAGPAGTKQQGSVKEISLSDTAVVMAYASKVYIVPGYGLAVAQAQHACHE
                     LEKLLEAKGVEVKYAIHPVAGRMPGHMNVLLAEADVPYDKLQEMEHANEEFAGTDVVL
                     ILGANDVVNPAAKTDPASPIYGMPILDVELAKNVIVNKRSMKPGYAGIENDLFFRPKT
                     SMLFGDAKKVLQDLIAEIKNL"
     gene            complement(10613..10930)
                     /locus_tag="PLU11_05605"
     CDS             complement(10613..10930)
                     /locus_tag="PLU11_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPN58623.1"
                     /translation="MDSILTWIAQNQQIIYIVILMIFVGIEVIGHVPSVLHTPLMSGA
                     NAIHGVVIIGAIIVMGKAESDNYLALVLGFLAVILGTINVVGGFVVTDRMLEMFKKKK
                     KNG"
BASE COUNT         3080 a         2733 c         2346 g         2811 t
ORIGIN      
        1 tcatccagtt tgttcagggc gttgttaaat gtccgtccca tctcttccga taaaacaagg
       61 tttcctttca tagcgcctaa ttgtagttta accccactta gtaatccccc aagaccatca
      121 tgcagatccc gggccatccg gttccgctcc tcttcctgtc cgcggagcaa tgactgtgtg
      181 gctgataatt gtttttctct ttccaggtca gcaatccgct gatcagacag ctttcttttc
      241 tgccggtaat ttcggtaagt tagcagggag atcaggagta aagcaatcgt acttcctata
      301 aatacatagt tcagcatact cttctgcctg atggaggatt gctgtaacaa caactgttgc
      361 tctttttgct ttccctgaaa ctttgcttcc gccagcaaga tggctgaatt acttttttct
      421 cccagcaatg aatcactaag cagctgaaac tgcttctggt aatccaatgc tgctttaaag
      481 ttgcccatct gctcgtacaa accagatgct tccaggcagt aattttttag ttcaagcgag
      541 gcattgttct cccgggcaat ccgtatggcc tgcaacacat aatagacagc tgaatcccgt
      601 tgtccgattc cggaaaagga agacgccaat cccccattcg catacatggc atacaccgga
      661 tcttcatttt cacccgccag cttcagggtc tgatggaaat agcggacact tgttttgagg
      721 tccttacggg ccaggaacac ttcccccaaa ccgtttaaac catccagccg gagaatataa
      781 tcctccgttt gctgtccgat tgtaaatacc tggttataca aggcaattgc ttcttcaatt
      841 tgacctttat tggttttccc tactgccagg ttaatcaggc aagaagcaat cgaatagtca
      901 tctttcatct gccgggcaat gaacagcgct ttgctagcgt aggcaatcac tttatcccag
      961 tctttgagct ctataaaaac ggaagccagg ttgttcagcg ccaccctttc aaatttcttt
     1021 tccggcaagc cggacgcctg ttcagcggcc ttcagataaa atgcagcagc tgtttcaaga
     1081 tcccccaggt attgccattc attagccaca ttgatataag cagtggcctg atcccggata
     1141 ttcccgaact gagtagcctg ttcaacagat tcctgcgtaa gcgccagtcc ttcgaggtat
     1201 ttccctttgt tattcaataa tacaatggca taactggtgt atgaaaaaat ccccttttga
     1261 tagtttaatt tctttgccag ttccagccct ttcctgaaat aaaaatcggc agagtcaggc
     1321 tttgactgtt cataaagctc accatactga taatagcgga atacttttat actatcttcc
     1381 gtcccgccct gcaggagatt gcggatactg tccgccgccc cggattgggc atatacccca
     1441 ttcgctgcca acaaagcagg aaaaatcagc atcaggattc cggttcgtat atgcataaat
     1501 attattagta acttaaaatt cacatacctg cttcataatt ccaagtttta ctttaaaatc
     1561 ccctgttcag gggatgaaaa aacatggatt caggggattg tccgttcttc taaaataaag
     1621 aagatttgta catgaaaagc aaccacatga aaccgaaaaa cactttcctc atttccttgt
     1681 ttctgttaac cggcctttcc atggaaggtc agttaaaaaa ctacgatatt ttttcctacc
     1741 aggcacccaa gggatttagt cttcaaaaac tggatgccgc gctgttttat tcaaaaaaag
     1801 acagcaaatc ctattgccag ttgtttctgt atccggctac tgcatctgtc ggaactccgg
     1861 atgcagattt cgatacaaac tggaacagct ttgcccgaaa cagcgggcag ggagtgaatg
     1921 atcctgaaac caaagaaacg gatacagcca acggatggca tataataatg ggagctgcca
     1981 aaggacggtt tggaaacaaa tcatatgtgc tcaccctgac taccctgagt aacggggaaa
     2041 tcagctttta caccgcttcc gttttttctg accagaaata tttaccggct gtccaggact
     2101 ttaccaatag tatacaacca gacctccaac tgtataccag caagaaaacc actactgaac
     2161 aatcttccac cactcaaaat caatctgcat ctcatacctc cggcaagagc attactaaat
     2221 ccaccacccg ttttgatgat ggctggatgg ccacccccaa tgataattat gtccaactgg
     2281 taaaagaaaa tactgaagtc cggctgatgt atatcaataa tgcattggat caggccaggc
     2341 cgaatacagc agatgccgcc gattattact ggaatgagta tgttagtcca tactttcaga
     2401 tagcacaagt ggaaaaatgg agcggggtgg aatacccggt catttatttc atgcaaggca
     2461 gcggcatcaa tctgtcaacg ggcaaaccct gccatgtggc cctgaaaata gtgtatagtg
     2521 gcggggcccg ccctatcctg gtcattgctt ccgatcagcc acaattccgt aagcaatacc
     2581 cccatccaaa tgaccttgac cgcatgctga attataacaa attcgccctg tcagccgctg
     2641 atctgaccgg gaaatggaca ggtggttccg gcgctggtgt tgaatactac aatgcctata
     2701 acgggacgta tgccgggatg agtgccatct ccacttctga tgatttccat ttcaatgcag
     2761 acggcactta taccagtgcc taccgatctg ccagttccag ccagggtagt acccgtttcg
     2821 gtgccatcga ttataaaggt aaatatcagg taacagactg ggctatccgg gcagaaaacc
     2881 ggtacaaagg aaaaacaacc cagttcaatg cacagtttat tgcggtgaag aacgggtacc
     2941 tgctctattt gtccgatagt gaaaaccctg ctatgaatta tacgcttata aaaattgctt
     3001 tatgaaaata actttcttag ccctgttgct ttgttcttgc atcatgacta tggcccaggt
     3061 caactgcgct acactggaga ttaataaagt accgggaaaa tgggtctggc aaagagcagg
     3121 ttatggtgca caatggcaat atggtgatcc gctcagaaag gagatgcaac gcatcatgcc
     3181 tgtggcactg gacggcttac atgccaccag cagtattgcc tttggcgatc aggccacctt
     3241 ctggtatacc aaaagcccgg ctgcgtatga gtattacctg atgctgaaaa aatatgaatg
     3301 cctcaaagga tataatacat tacaacagga aggagaaaca agctgctggg tacatttttc
     3361 ggttaaccag cttgatggag aaaaattccc catgcccgag cagggaaccg gggtgatttt
     3421 taacgaatca agaatccggg ttaccaatat tgaggtgcag accgatgcag ccgggaacaa
     3481 agtgatttac agtaattaca gaccggatga aaccatcaaa cattgttatt ttttttcatc
     3541 cagaaaaaac ctgccctgga gaaagctgaa caacaaagaa ttattcacgg cttacaaagc
     3601 ccatcatgaa accagagtga taaaggaaat agcccggttt gaaaaactgg tatccgatga
     3661 tgagaaaaaa ttcaacagcc tttcttccgc caaaaagcag caacaaacct attggccgga
     3721 aatcatccgg aaaaacaaag agatactgca gcgttataaa gacgaacata aaaaaattat
     3781 cacctggtat accgccgcat taaaacagcc agatcttcag tctaccgcct atgttatcag
     3841 catcaatgag tcgcattttt atccggataa actaacagcc agtccgggtc acggctacta
     3901 tgcctgggtc gataatctgg atttttttga taaaacaaaa ccaaaagacg aaccacaatg
     3961 cattgcatta tacccacgaa agcaggatac agatattccc aaaaagaatt tcatggagct
     4021 gttttacagt caatttaacc tggatgtact aggcaggatg gtgggtgagc ctgcgaaaaa
     4081 gccaggcggc atcaattcat taaacgcttc tttgaatagc gctaaggccg gttcagcaat
     4141 aaaccaaacc accattaaca actaccgata cagttttgac cagagtgaaa taaaccggtt
     4201 cccgcaagga tggaacggcg tgaaaaacag ccaggtgaaa ccatttgaaa ataataactg
     4261 gctggcacta accagggacg gttactggta tccacagcaa tataataaag atataaaaga
     4321 cagattcagt ttatcgtttg acctggcctg gaataaggat attgcatata atagcggatc
     4381 atttacagtc accctggcag aagtggatta tgacaatacc ggagaacgtt accggctgga
     4441 tgataatcaa aacatgtatt ggagtctgta tgatagttat gttggtaaat ttaacagggt
     4501 aatgctgtgg tttgatcctt actggaacgg aggtggatta ctgacagttt actcctatga
     4561 taaaacagaa aacatcaagg taaacaaacg cataacactc cctgatttct atcttacaaa
     4621 aaatgtacat caactgaaag tacaacgggc cgggcaatcc ctcatcatca ccatcaacaa
     4681 caaaaaggaa gcagaaattc ctgacgtatt tctcccttcc gttaaataca atctttacac
     4741 gttcagccgc tacaaagggg ataacagtga taataaaaac gatgtgttct acctgaaaaa
     4801 cattgtggtg gattattaaa gacaagacag gttcagcaga atggaaccct gcacaaccct
     4861 aagaagatat ggtggctgat ccgggtataa tccggcttta cttaccttcg tgctatggat
     4921 cagctcaacc gtttacggat tgtatttatg ggaacacctg aatttgccgt tgccagcctg
     4981 gatgccctgg ttagagcggg ctgccaggta gtgggtgttg taaccgcccc ggataaaccc
     5041 gccggacgtg gcatgcagct gcagcaaagt gcggtaaaaa aatatgcggt tgacaagagt
     5101 atacctgtgc ttcagccact gaagctcaaa gatcccgaat tcatcaacga attaacggca
     5161 ttaaatgccg acctccagat cgtggtggct ttccggatgc tcccggagat agtctggaat
     5221 atgcccccga tgggtaccgt caatctgcat ggcagtctgc tccctcaata ccggggtgcc
     5281 gcgcctataa actgggccgt gattaatggg gaaaaagaaa ccggggtcac tacatttaaa
     5341 ctcaaacatg aaattgatac cggtgacatc ctattgcagg aatcattccc gataacagaa
     5401 caggatacgg ccggcactat tcacgaccgg atgaaagaga tcggggcttc cttactggta
     5461 aaaacggtta ccgggctggc ggcaggcact ttaactgaaa agccccagac cggcaatccg
     5521 gatatgttaa agcatgcccc gaaaatccat acggatacct gccggatcaa ctggagtctg
     5581 actgccggag aaatttataa ccgggttcgc ggactcagtc cccatccggc cgccttcacc
     5641 atcctcaatg aaaaaatgct gaagatattc cgttgtgaaa aagaagtagt cagcccgaaa
     5701 agttcagccg gcgaatatga gacggatgga aaaaccttcc ttaaaattgc ctgtaccgat
     5761 gggtatattc acctgctcga tatccagctg gaagggaaaa agaaaatgga gatcactgaa
     5821 tttctccgag gttaccgatt tgcctaaaac taacgtaaat gaatattcgc aatttagttc
     5881 tgctaatggc gcttaccagt tgcctgacgg tatcggccca gaaaaataaa aagtggaaat
     5941 ccctgtttaa cggccggaat ctgaaaggat ggaccgctaa aattacgggc tatagcctgg
     6001 ggaataattt cggaaatacg ttccgggtga aagacggcaa actggtggtt tcctatgacc
     6061 agtatgaagg atttaataac cggttcggtc atctctttta caaaaaaccc ttcacacatt
     6121 acctgcttgc cattgagtac cgttttaccg gcacccaggt cagtggtggc ccttcctggg
     6181 cgtataaaaa cagcggggtg atgctgcatt gccaggcgcc atccagtatg accactaatc
     6241 aggacttccc catttcactc gaaaaccagt ttctcgaagg agattccacg ggtgtccgtc
     6301 caacctgtaa cttatgtacc cccggtactg aggtaatgat caatgggaaa aaaccgtcgg
     6361 aacactgtat caaatcctcc tctcccactt accgtaacgg ggaatggatc cgggcagaaa
     6421 tgctggtact cggggattcc gtgattaaac atattgtaaa cggagatacc gtgctggtct
     6481 atcatcaacc cagtatcgga ggtggcgtag taaacaattt tgatccgtct gccaaaccgg
     6541 atggaaaacc cctgacgggc ggatatatcg ccttacaaag cgaaagttcc ccgattgagt
     6601 tcaggaaagt ggagatcatt gacctcagca aaaaatacaa gcggaagaaa atcagtaagt
     6661 gatcttcacg ataaattttt ctgtatcggt gatggccagt gcagaggcag cggcattgct
     6721 gatgcggata gtataccctt cattctgacg gatcccattc atttccccca ggactttggc
     6781 aaaaatcacc cgggtattgg aaggattgct gatccggata atggtaccag gggccacccc
     6841 gtcaatcagc atataatatt tttgatcctg ccagccactg gccgttttga atataccgga
     6901 agtcaccgtt tcttcttttg acaaaggcgc ttttttcacc tgttgattaa atgaaacctt
     6961 aaagtaccct tgtgcattat ctccggaact actcacgggc tgtgcgaccg gcttttcctc
     7021 tttcttttcc ggctgacgta tttctggttg ctttatcact tccggttgcc tggtttcagg
     7081 ctgaggcgcg ggttccacca ccggctttgc cggctccttt ttctcctcca ccagcaccgg
     7141 gggcaacggt ttgttctcca ctttatcctc agttgccggt accggttttg ccttgatggt
     7201 cactgaaggc atttccttac tgagcaggaa gccgatgatc agcttattgt ctttcttcgc
     7261 ctggtcaccg gataacccat tccaggattt caaactcgta gccggaacat tattgtgtgc
     7321 gcggctgata tcagccagag ctattactgc ggacggttta taatacaccg gggtgccact
     7381 gtttcccttt tgggtaaaat tggtatcggt cagcggaatc cgaagacgct gatcaatctg
     7441 taacccttta ttaatatcca gtttattata tgaagcaata tgccggggat gtacattgta
     7501 caaacggccg atggaaaaaa aactttcttt cggggctacc ttatgttcca gacaaagctc
     7561 cttctcacct gacttcacat ataagtcggc cttttgcgcc tgcaggagaa tagaagaaaa
     7621 caacaaaacc agaactataa gaatgccatt cttcataccg acaagatttg tgagcaaaga
     7681 tgcagttttt ttagcgtttt gcgggctaaa tatccttcag gatacggagc tccttgggat
     7741 aataattatt gatccgcccg ggaagaatat tcatccagcc cagtgattta ccggcaaaac
     7801 aagcccgtga ccagcctgtt ggaactcctt ccagccggat ttcctttcgt tgcaggtacc
     7861 ggatggcatc ttccgccact aaatcaatcc cgggtacatc ggcaagaacc aggccactca
     7921 tagccaacga atgatccgga accattttat cccgcatcag ctcacccgac ttcaccccgg
     7981 aatagatcac cctcattttt tccaataaaa ttgaaaaatc ttgaaaatgg tcttcgggcc
     8041 aggcatacac ggtctgctgg tatttgatca attccttacc cgctatattc atccaacggg
     8101 ccacccactc tttttccgcc cgggtcactc gttccggttg cttcttcgaa ttcatttttc
     8161 ctggttcatc tccattcctt ttccggaaac aggcaaggaa aaaaccttcc ccatttatcc
     8221 gatccggcca gaatcgatac cccaggctgc ctcccggaga ttctgtttca actatgcccc
     8281 attcttcctt tatagttaat ggacaattct ccgcatccag ttcctgttta atccaatcca
     8341 taatttcttc atcttcttcc cgggaatagg aacaagtaga atagatcagt aacccgtttt
     8401 cttttaaagc aggccagcaa tcggccagta tccgttgctg ccgctgtgaa cataactgca
     8461 cattattgag actccactca ccgatcgctt ccggatcgcg gcgaaacaaa ccgctgccac
     8521 tgcagggcgc atccaccacc agcacatcaa aaaaactctc cagccccgca aaatctttcg
     8581 ggtcattatt ggtaaccact acctgttcag ccccccattt tacgatatta tccctgagca
     8641 cagctgcccg ggaacgtatc acatcattgc tgaccagcaa actgccagca ggtagcaggg
     8701 attgaagatg ggtggatttg ccaccagggg atgcacagag atccagtact ttaaccggct
     8761 ctgcacgttg agtgagttgt agcagggcct gttccagaaa catcgaagaa gcttcctgca
     8821 cataataatt acccgcatga aacagcggat caaacgtaaa tgaggggcgt tgcctgagat
     8881 agtaaccgag gccggtccag ggaatcgctt ccgccagtga gacaaattcc gggggtagta
     8941 ctccatccgt cttggctgga ttgagtcgta ctgaagtgac ctgtttacct gactggtgaa
     9001 cctgttcaaa ggcttccggg gaaaacccaa taatgccctg tagtgatttt aaaaatgcgg
     9061 ctggtaaagt cacccggcaa aactaggtac aataatttta tttctgctgc atagctgtcg
     9121 ccctggctga ttataaattt ttgatctccg caatcagatc ctgtaatact ttcttggcat
     9181 caccgaaaag catcgaggtc ttgggacgga agaacagatc gttttcaata ccggcatacc
     9241 cgggtttcat actgcgttta ttgacgataa cattcttcgc cagttccaca tccagtatcg
     9301 gcataccgta gatcggggaa gccggatctg ttttggccgc cgggttcacc acatcattgg
     9361 ccccgagaat aaggaccaca tccgtaccgg caaactcctc attggcatgt tccatttcct
     9421 ggagcttatc ataaggcaca tcggcttcgg ccagcaatac gttcatgtgc ccgggcatac
     9481 ggcccgccac cgggtgaatg gcatacttca cttccacccc ttttgcttcc agtaattttt
     9541 ccagttcatg acaggcatgc tgggcctggg ccaccgccag tccgtatccg ggaacgatat
     9601 atactttact ggcataggcc atcaccacgg ccgtatcact taatgagatt tcctttaccg
     9661 atccctgttg tttggttccc gcagggccgg cttctttatg accaccacca aaagaaccaa
     9721 tcaatacatt ggtcagcgaa cggttcatcg ccttacacat aagaattgtc agcaaggtac
     9781 ccgcggcacc aaccagtata ccacccgtga gcataacttt attatcatac aggaatccac
     9841 cacaggcagc tgccaccccg gtaaatgagt tgagcaggga gatcaccacc ggcatatccg
     9901 cacctccgat tggcataacg aagaatacac catataaaat ggaaatggcg aagatagcat
     9961 agaagattac actaggattg gcaggtgtcc atccgattat ataaaccgcc agtgcgagga
    10021 tcaccagcaa caatccgata ttcagcaggt gctgacccgg aaatacgata tcctttattt
    10081 ttccattcag tttaccccag gcaatcatac taccggcaaa tgaaaccgaa ccaatgatca
    10141 gtcccaataa gatgatgagc agtgttggga tatgcgccga aagatctccg tctgccgcat
    10201 gcaccaggtg attgaactcc acaatactga tcagggccgc acaagctccc cccattccgt
    10261 taaacatact taccatttcg ggcatggcgg tcatcttcac tttttttgcc gccaatgtac
    10321 caacgatgga cccaatggca atccccccga atatccatcc gtaattaccc agctttaccc
    10381 cttcctcttc atagagaaaa atggtaccga taatggcgat cgtcataccc gccgcggcca
    10441 ccaggttacc ctttctggct gtagcaggat tggaaagcat ttttaaaccc agtataaaag
    10501 taaccgaggc gatcaggtaa caaaaggtca gtatactcag ttccattgta aatcttttaa
    10561 aattagtctt actagataca gcttacagca tcattacatc ctgtataacg gcttatccgt
    10621 ttttcttttt ctttttaaac atttccagca tacggtccgt taccacaaaa ccacctacca
    10681 cgttgatagt ccccaggatc accgccagga aacccagtac gagggcgagg taattatcgg
    10741 actccgcttt ccccataaca atgatggccc cgatgatcac caccccatgg atggcattgg
    10801 cgccactcat caggggcgtg tgcagtacac tgggtacatg accgattact tcgatcccaa
    10861 caaaaatcat caggatcacg atataaatga tttgctggtt ctgtgctatc caggttaata
    10921 ttgaatccat acgtttattt taatctttcg tgaatgattt gtccttcatg
//
LOCUS       DAOPMW010000074        10941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6422_length_10941_cov_11.808561, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000074 DAOPMW010000000
VERSION     DAOPMW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6422_length_10941_cov_11.808561"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLU11_05610"
     CDS             <1..810
                     /locus_tag="PLU11_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPN58624.1"
                     /translation="LKNVIPDKTKDTARQVVQKVVDQLMKKLMQPMQQAVTGSLNRST
                     RNRRPRHNEINWHLTIQKNLKHYQPDYKTIIPETRIGYGRRRTSLKDVVLCIDQSGSM
                     GTSVVYSGIFGSVMASIPAIKTRMVVFDTAVADLTEELSDPVDLLFGVQLGGGTDIHA
                     ALSYCQQVITRPTDTVLVLITDLYEGGEVDGMRKKAVELTAAGVQVVVLLALNDEGAP
                     SYDHTNAQFFSDLGVPVFACTPDKFPDLMAAALGKQDLAQWAAKEDLILKK"
     gene            820..3825
                     /locus_tag="PLU11_05615"
     CDS             820..3825
                     /locus_tag="PLU11_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HPN58625.1"
                     /translation="MAFRPEIIEPYFQHMLETNYNHYNPEPSLGFVKAFLTGETNTLT
                     PLPQNWFNEKFARLAELLINEQTSPENKQLLIQLFKDPEMYKNSRYKVAQYLVPYELD
                     LDKLTERYLRMKELLEQLGFSRIELGNMFANYASHFMNMEPVKSEEMLRNNSNPVLHL
                     VKEAHLSRTSQEIDELVAKKERRYYDFSVALATLLKIYNPEKYIEYLRYYEKTDTAPF
                     IADNVMHYCVKTDPEKYLSYYQNWIRQCGEQTGEDALKKLYLTLAISTYSPLTEEYNR
                     LVFKVLDLQTAPAAVLSDQSIDNYSSTFFPFTDKREYRHIQLLCLALKLTSDTDPVKA
                     IHYWNLLDEKNYCMEKSGFSYLYETLGKQTLPLLQKQLLKKTPSEYSEKERIEALTGI
                     LNNHTAEMDYSLFWNFGAYQSKACRNAVIPFLAEHDKEAEARGVEGLKGRTAEQRLNA
                     ARVLSILGTSGAMTALRNSIETEKDDETRDIMLVVAGDEYLAGITEGTLPAVIAAHKK
                     RGKLKKELAPWLEESVLPPLFFKSGKQLNNEEVRFILYRMSRVKDLATEAEIKPVIAC
                     IDKERSAGFAKEVLRRYKEAGYDAKFKFLLGVAALLGDEEMVGQLQKGIDEWIEGNRK
                     LMAELGIKALSLHQSNKALRVIEWYSRKYKSKKPNFGEAALQSLENAATELGITIHEL
                     GDRIVPDFGFLGLFRHFTVNGEEYRAFIDSKFKIAFFNEDNKKIKTIPAAAPDELKEE
                     FKYIGKEIRDIVKSQSSRLEYYLTIQRQWTPEQWRAFFLENPVMFIYATKLVWGVFNE
                     QEDLVTTFICNEDTSLLDEQGEEISLPETGKISIVHPGQLTPASLENWKRQLFDLSVE
                     QVFPQLDRPLPDMEGIDRSSRMIKKFLHTEMAVGSIRSTLERAGWFKGPTGDGGYLES
                     IKLLYFEKKLEAVLELDGVGVGYGWSNQERTGRLYFIDKSKIKSRYDSIRSDDDEKLI
                     PLHQLPPIFLAEVIGAIDRIKRAEKKTAF"
     gene            3956..6502
                     /locus_tag="PLU11_05620"
     CDS             3956..6502
                     /locus_tag="PLU11_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58626.1"
                     /translation="MKRLLLAGCLWPALSGAQEVKPVYAADYQALIQNDSNNLRINDS
                     SNSMRTARLKVPGNIYYINYRIRKISIGKDSTLVLKPAGDNTLFISGSWSTTAEMQRV
                     NRMPALQKEYAQGHSVNGQLNWLGPETNELFSYGPAMQLLEFDGSNYLYDQNGRLVNA
                     GTGNGQPTTVYNNSLLRPATFFSNNLGINFKYPNIYGKYVAAGFKAGQTKEKTFIREN
                     NNKYRFYTATLDASLPNISLNNSYNYRDEWYDHSNRNGFLNRLYQQSLLTPVSFSNAQ
                     GSLLNNGQRSYSQFADNPLFLLDNPSSGFRREQHSTAVSAEYRLKNLKIKFGQSIENN
                     RQTSHESLQPGTAGFPTGRVLNRFQLDKNYSLQSNISYSFRWGSNNDYAAVNLNHIYA
                     DNRTSIRYGIAHPDYQYQRTSQDLSLSFSPSRQGDNSESGFNLGYKYFTSNTSRSHYW
                     LPMASGFHRFNNLFNESQLSLKLYTSYNRFNSELPVSKSLVAVSLLQLDPEEANQYNP
                     VLEVNSYSGLRPVEHCEFQAGMEIRVGYRFSLSGNWFNRNIRNDIFPFIQNNEIQLRN
                     IAGHYNRGIELQFDYNPWLNNAAQLKAGFTLSFMRYQSKVTGLQTGFEKLPLTGFRSV
                     HTALVKGEPVGVIMGHGWLRNANNQVLIGSDGFPLMDQQLKVLGNPIPDFVLKWSDKL
                     RYKNWDLAMDFEWEKGGDSWNGTQAMLDYYGRSAVSAVQRGTRNYVFEGVLANGSHNT
                     IPVSFYDPSLPFSQNRWVRYGTTGVTEEYIQKADLLRISTLSLSWKKVLKKYLQQIQI
                     SAYANNILLWSPYKGADSNQLLFDQSGTQGLDFFNLPAVRAAGISLALQF"
     gene            6507..>10941
                     /locus_tag="PLU11_05625"
     CDS             6507..>10941
                     /locus_tag="PLU11_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN58627.1"
                     /translation="MKKIFIIATWFLITFLPVIVYAQDFASRKEKIYIQTNHSFFNVG
                     EVVYFKAYVVRAENNTPSLISNVVYAELISPSGTIAQKGNFQATDGFAEGSFLFPADA
                     PGGIYKIRAYTTWMQNEKESSFFVKEITLQKVLAPRILMKLDFPEKGYGAGDRVKADF
                     SMRNLQDQPIRNYAGKFTVFISGNALLTENFSTNAEGKATILFTLPRPLTVNDGLLNV
                     TIQYESFTESISRSIPIVLNNIDLQFMPEGGTLVEGLSSWVAFKALNENGKAADIKGE
                     IRDEQGKTVTTFGSYHFGMGKFLFTPQTGHVYKAYITSPTGIATTYDMPVASLNGILM
                     HLEKQNHTITVQLNASVNMEVKLRGMVRNTTWYTMSIQVKKGVTRFTIDEKIFPAGIA
                     RFSLTAANELPLAERLLFLNEDRQLKVNLQFDKSSYAPREKVKLSIHTTDPEGKPVPS
                     NLSIGIVDDKLWTFADDKQDHILSWLLLSSELKGKIEEPQFYFKKEEPKAIPALDLLM
                     LTHGYRYFDFIEYVVKEGMLKYLPDQDNIVSGRIQDLNGNPVQAKMFLMNRVYGGRAM
                     EYTTGADGVFFFSDLISNSSYYLFALPLKSTGNVVINILQNGLGYNPAKTRALNMLAA
                     KPKVFEGIGISPLPVAGKKELEAKKQDVLFNNRQSALNEVVVVGYGAQRKASLTAAVS
                     VVRADEIGTTAQLGNMLQGKVAGLQITQSGNFLQTPSIRLRGSSTVTGNHEPLFILNG
                     VPMDGFSLNTLNPNDIDNITVLKDGNATALYGYRAAYGAIVIESKKLRRERIKLKFKN
                     PTYYTSQQFNTSGTSYTPVRRFYAPLYETVKTEERSDFRETIYWNPVVQTNKEGKAEI
                     DFYNSDASTTFRAVAEGIGFNGIPGRTENTYAVQSNLRLDAKIPPYLTVGDKALIPLV
                     IKNNSGQSGTAGIEVVTSADFITGVYTRNYFLEPDSSVRLLIPLEARRAGQGQIQFTV
                     KSEFGNETLSLPVSAAEKGFPVSLVFSGNKSGQHEFPISRMVPGTLRTKLQLFSSMEG
                     QLLDGIESMLREPYGCFEQTSSTTYPNVFILKYLKESGKSNPKIERKAMDYIKRGYNR
                     LIGFETKQDGFEWFGNTPPHEALTAYGLLEFTDMKEFIDVDEKMLTRTKNFLLSRRDG
                     KGGFTIQKKGYDQFASVPDKIATLYIIYALTEAGLTKEVETEYRAGVKKALESKDTYQ
                     LALMALAANNMNDQESYQRLMRLLADQPEKGKMETSVVNSRDASLRVETWSLQALALL
                     RANQPDMGRVANLLSKILGEKSYYGYGSTQATVLALKAITAYGRLAGKVAGETEIDFT
                     LNKDRVSHYDSLNSLLQTGNNSFNVVYRKNENAVPYNLEVSYSTFIPPSADKAEISLI
                     THLKSGTSLTGETVRMDIEVTNTRSVLQPMAIAKIGIPAGLSIQPWQLKEFIEKNKCA
                     YYEIFDNYLVFYWMGFAPNETKTLGLDLKAEIPGTYKAKAST"
BASE COUNT         3219 a         2523 c         2551 g         2648 t
ORIGIN      
        1 ctgaaaaatg taatacccga taaaacaaaa gatacggccc ggcaggtggt acagaaagtc
       61 gtggaccagc tgatgaagaa gttaatgcaa ccgatgcagc aggcagttac gggcagcctg
      121 aaccgaagta cacgtaaccg tcgtccgaga cataatgaga tcaactggca tcttaccatt
      181 cagaaaaacc tgaagcatta ccagcccgat tataaaacga tcattcctga aaccaggatt
      241 ggttatggcc ggcgacgcac gtcgcttaaa gatgtggttc tttgtattga ccagagtggt
      301 tccatgggta catccgttgt ttactccgga atcttcggtt ccgtgatggc atcgatcccc
      361 gcgattaaaa ccaggatggt ggtctttgat accgccgtag ctgacctgac agaagaactc
      421 agtgatccgg ttgacctgtt attcggggtg caattgggtg ggggtaccga tatccatgcc
      481 gccctcagtt attgtcagca ggtgatcacc cgtcctaccg acaccgtgct ggtactgatt
      541 actgatctgt atgagggcgg agaagtggac ggaatgcgga aaaaagcggt ggaactgaca
      601 gccgccggtg tacaggtggt ggtattgctt gccctcaatg atgaaggagc tccttcctat
      661 gatcatacca atgcccaatt cttttctgat ctgggtgtac cggtctttgc ctgcaccccg
      721 gataaatttc cggatctgat ggcggcggct ctgggcaaac aggacctggc acaatgggcc
      781 gctaaagaag acctgatttt aaaaaaataa agtaccctta tggcattcag acctgaaatt
      841 attgagccct attttcagca catgctggaa acgaattaca atcactacaa tccggaaccc
      901 agtctcggat ttgtaaaagc cttcctgacc ggggaaacaa acaccctcac cccgcttccg
      961 caaaactggt tcaatgaaaa atttgcgcga cttgctgagt tattaattaa tgaacaaacc
     1021 agcccggaaa ataaacaact gctgatacag ttgtttaaag atcccgaaat gtataaaaac
     1081 tccaggtata aagtagccca atacctggtt ccctatgaac ttgatctgga caagctgact
     1141 gaaaggtatc tacggatgaa agaactgctg gagcaattgg gtttcagccg gattgaactg
     1201 gggaatatgt ttgcgaatta tgcgagtcat ttcatgaaca tggagcccgt aaaaagtgag
     1261 gaaatgctgc ggaataacag caatccggtc cttcacctgg tgaaagaagc acatctgagc
     1321 cggaccagcc aggagataga tgaactggtg gcaaaaaaag agaggcgcta ttatgacttc
     1381 tccgtagcat tagccacctt gttgaagatc tataacccgg aaaagtatat tgaatatctc
     1441 aggtactacg aaaaaaccga taccgctcct tttattgctg ataacgtaat gcactactgc
     1501 gtaaagacgg atccggaaaa atacctgtcc tattatcaaa actggatccg gcaatgcggg
     1561 gaacaaacag gggaggacgc attgaaaaaa ctctacctga cactggccat ctccacttat
     1621 tcaccactta ctgaagaata caaccggttg gtatttaaag tgcttgacct gcaaactgcg
     1681 cccgcggcag tgctgtctga tcaatcaatc gataattatt catctacctt tttccccttt
     1741 actgataaaa gagaatacag gcatatccag ttgctatgtc tcgccctaaa gctaacgtcg
     1801 gatacagatc cggtgaaagc catccattac tggaatttac tggatgaaaa aaactattgc
     1861 atggagaaat ccgggttctc ttatttatat gaaacactgg gtaaacagac cttgcccctg
     1921 ctgcaaaaac aattactgaa aaaaaccccg agtgaataca gtgaaaaaga acggattgaa
     1981 gccctgaccg gtatccttaa caaccataca gcggaaatgg attacagcct gttctggaat
     2041 tttggcgcat atcaatccaa agcctgcaga aatgcggtta tccctttcct tgctgagcat
     2101 gataaagaag ccgaagcccg tggcgtggaa ggattgaaag gccgaacggc agagcaacgg
     2161 ctgaatgcgg cgagggtttt atccattctc ggtacttctg gtgcgatgac tgcgttacgt
     2221 aacagtattg aaacagaaaa agatgatgag acacgggata tcatgctggt ggtagccggt
     2281 gatgaatacc tcgcaggaat cactgaagga accttgcctg ctgtcattgc agcacataaa
     2341 aaaagaggta aactgaaaaa agagctggcc ccctggctgg aagaaagtgt actccctcct
     2401 ttgtttttca aaagcggcaa acagctgaac aacgaggagg tccgttttat cctttaccgg
     2461 atgtcacgag taaaagacct ggcgacagaa gcagaaataa aaccggttat agcctgtatt
     2521 gacaaggaaa ggtccgccgg ctttgccaaa gaagtgttgc ggcggtacaa ggaagccggt
     2581 tatgatgcga aattcaaatt tttgctgggt gtggcggcat tactcggcga tgaagaaatg
     2641 gtagggcaat tgcaaaaagg aattgatgaa tggattgaag gaaaccgtaa actgatggcg
     2701 gagctgggta tcaaagcgct ttcattacac caaagcaata aagctttgcg tgtcattgaa
     2761 tggtacagcc gcaaatacaa atcaaaaaag cccaatttcg gggaagccgc gttgcaatca
     2821 ctcgaaaatg ccgccactga actgggcatt accattcatg aactcggtga ccggattgtg
     2881 cctgacttcg gtttcctggg attgttccgg catttcaccg tcaacgggga agaataccgt
     2941 gcgtttattg acagcaaatt caaaattgcc ttcttcaatg aagacaataa aaaaataaaa
     3001 accattccgg cggcggcacc tgatgaactc aaagaagaat tcaagtacat cggaaaagaa
     3061 atccgcgata ttgttaaatc ccaatcttcc aggctggaat actatctgac gatccagcgg
     3121 caatggaccc cggaacaatg gcgggctttt ttcctcgaaa acccggtcat gtttatctat
     3181 gccaccaaac tggtttgggg tgtttttaat gaacaggaag acctggttac cacattcatc
     3241 tgtaatgaag ataccagcct gctggatgaa cagggagaag agatttcact accggaaacc
     3301 gggaagataa gtattgtgca tccgggacaa ttgacgcccg catcacttga aaactggaaa
     3361 agacagttat tcgatctttc ggtggaacag gtttttcctc aacttgatcg tcccttaccc
     3421 gatatggaag gaattgatag gtcttccaga atgatcaaaa aatttctgca taccgaaatg
     3481 gcggtcggat ccatccgcag cacactggaa agagcgggat ggtttaaagg ccctaccggt
     3541 gatggcggtt atcttgaatc gattaaactg ctctatttcg aaaaaaagct ggaagccgta
     3601 cttgaactcg acggtgtagg cgtaggttat ggctggagca accaggagcg gaccgggcgc
     3661 ttatacttta tcgataaatc gaaaataaaa tcccggtatg attctatccg aagtgatgat
     3721 gacgaaaagc tgatccccct tcaccagctc cccccgatct tcctcgccga agtcatcggt
     3781 gccattgacc ggataaaaag ggccgaaaag aaaacagcct tttaatattg atgtcaaaac
     3841 ccggttccaa caccgggttt ttcttttttt gcagatctgc caaatctgta tgtaatccct
     3901 tttccccctg catccgttaa gaaagagtga atttttaacg ccaaaaccag ggattatgaa
     3961 acgattactg ctggcaggat gcttatggcc tgccctgtcc ggtgcacagg aagtaaagcc
     4021 ggtttatgcg gctgactacc aggccctcat ccagaatgac agcaacaact tgcgtattaa
     4081 tgattccagt aacagtatgc gtacggcaag attgaaagta cccggcaaca tctactatat
     4141 taattatcgc atccggaaaa tcagtatcgg gaaagacagt acattagtac tgaaaccggc
     4201 cggagataat actcttttca tcagtggttc ctggagtaca actgcggaaa tgcaacgggt
     4261 aaaccggatg ccggccttac aaaaagaata tgcccagggc cattcggtca acggacaact
     4321 aaattggctg ggccccgaaa ccaacgaatt attcagttat ggtcccgcca tgcagctgct
     4381 ggaatttgat ggcagtaatt atttatatga ccaaaatggc cggctggtaa acgcaggtac
     4441 cggaaatggt caaccaacca ctgtttacaa taatagcctc cttcgccccg ccaccttttt
     4501 ctccaataac ctgggcataa attttaagta cccgaatata tatgggaaat atgtggccgc
     4561 cggtttcaaa gccggacaaa caaaagagaa aacctttatc cgggaaaaca ataacaaata
     4621 ccgtttttat acagctaccc tggacgcttc attaccaaat atcagtctga acaacagtta
     4681 caattaccgg gatgaatggt atgatcacag caaccggaat ggatttttaa accggctcta
     4741 ccagcaaagc ctgcttaccc cagtcagttt ttcaaatgca cagggaagtt tgttgaataa
     4801 tggccagcgg tcttatagtc aattcgctga taacccactc ttcctgctgg acaacccatc
     4861 aagtggattc cgccgggagc aacatagtac agccgtcagt gctgaatacc gtttgaaaaa
     4921 tctgaagatc aaattcgggc aatccatcga aaataaccgg cagaccagtc atgaatccct
     4981 ccagcccgga acggctggtt tcccgaccgg cagggtactc aaccggtttc aactcgataa
     5041 aaattattcc ctgcaatcaa acatttctta cagtttccgc tggggcagca acaatgatta
     5101 tgcagcggtt aatctcaatc atatttatgc cgataaccgg acttccatcc gatacggaat
     5161 tgcacatccg gattatcaat accaacggac ttcacaggac ctttccctca gtttttctcc
     5221 gtcccgccag ggagacaaca gcgaatccgg tttcaatctt ggctataaat attttacctc
     5281 caataccagc cgcagccatt actggttacc gatggcttcc ggctttcacc ggttcaataa
     5341 cctttttaat gaaagccagt taagtctgaa actctatacc tcctataacc ggttcaacag
     5401 tgaattaccc gtgagcaaat cactggttgc ggtgtcttta ctgcaattag atccggaaga
     5461 agcaaatcag tataaccccg tactggaagt aaattcttat agcgggttac ggccagtgga
     5521 acactgcgaa ttccaggcag gtatggaaat aagggtcggt taccgattca gtctgagcgg
     5581 taactggttc aaccggaata tccggaatga tattttcccc tttattcaaa ataatgaaat
     5641 acaactccgc aatatagcgg gtcattataa ccggggaatt gaacttcagt ttgactataa
     5701 tccatggctc aacaatgctg ctcaactgaa agccggtttt acgttgagct ttatgcgcta
     5761 ccaaagtaaa gtcaccggtc tgcaaaccgg atttgaaaag ttgccgctga ctggcttccg
     5821 ttcggttcac accgccctgg taaaaggaga acccgtgggt gttatcatgg gtcatggctg
     5881 gctgaggaat gctaataatc aggtgctgat tggatcggat ggttttccat tgatggatca
     5941 acaactgaaa gtactgggca acccgattcc ggatttcgta ttgaaatggt cggataaact
     6001 ccgctataaa aactgggatc tggcaatgga cttcgaatgg gaaaaaggcg gggatagctg
     6061 gaatggcacc caggccatgc tggattatta tggtcgctct gctgtttctg ctgtacaacg
     6121 cggaacccgc aactatgtat ttgaaggggt attggccaat ggcagtcata ataccattcc
     6181 ggtttctttc tatgacccct cccttccctt ttcacaaaac cgatgggtcc ggtatggtac
     6241 tacgggtgtc actgaggagt atatacagaa agccgacctt ctccggatca gtacgctgag
     6301 tctttcctgg aagaaagtgc tgaaaaaata cctgcaacag attcagatct cagcttatgc
     6361 caacaatatt ttactatggt ctccctacaa aggagccgat agtaatcagt tactgtttga
     6421 tcagtccggc acccagggac tggatttctt caacctgcct gctgtacgcg ccgcaggtat
     6481 cagtttagct cttcaatttt aatgctatga aaaaaatatt catcattgcc acatggttcc
     6541 tgatcacctt tcttcctgtg attgtttatg cccaggattt tgcctcccgc aaagaaaaaa
     6601 tatatatcca gaccaatcat tccttcttta atgttggtga ggtcgtttac ttcaaagcct
     6661 atgtagtcag ggcagaaaat aatacaccca gcctgatcag caatgtagtt tacgcggaac
     6721 tgatcagtcc ttcaggaacc attgcacaaa aaggaaactt tcaggccacc gatggttttg
     6781 cagaaggatc ctttctattc ccggcagatg ctccgggagg aatttataaa atcagggcct
     6841 ataccacctg gatgcagaat gaaaaagaaa gcagcttctt tgtaaaggaa atcactctgc
     6901 aaaaagtact cgctccacgg atattaatga aactggattt tccggaaaaa ggatatggcg
     6961 ccggtgaccg ggtaaaagca gatttttcca tgcgcaacct gcaggatcag ccgatccgta
     7021 actatgccgg gaagttcact gtattcatca gtggaaacgc gctcctgacg gagaacttca
     7081 gtaccaatgc ggaagggaaa gcgacgatcc tgtttaccct gccccgaccg ctgactgtta
     7141 atgacggatt attgaacgtg accatccagt atgaatcctt taccgaatcc atttccagga
     7201 gtattccgat tgtactcaac aatatagatc tgcagtttat gcccgaaggc ggcaccctgg
     7261 tggaaggact cagcagctgg gtggctttca aggccctgaa tgaaaacggt aaagctgccg
     7321 acattaaagg agaaattcgc gatgagcagg gtaaaacagt caccacattc ggctcttatc
     7381 attttggcat gggaaagttc ctctttacac cacagacagg ccatgtctac aaagcctata
     7441 tcacttctcc cacgggtatt gcaaccacct atgatatgcc cgttgcttcc ctgaacggga
     7501 ttctgatgca cctggaaaaa caaaaccata ccatcacggt acaactgaat gcttccgtaa
     7561 atatggaggt aaagctgagg gggatggtgc gcaataccac ctggtataca atgagtatcc
     7621 aggtaaaaaa aggagtcacc cgctttacga ttgatgaaaa aatttttcca gccggcatag
     7681 cccgtttttc attgacagcg gccaatgagt tgcccctggc agagcggctg ctgtttctga
     7741 atgaagaccg ccagctgaaa gtgaatcttc aatttgataa gtcttcatat gcaccccggg
     7801 aaaaagtaaa actcagcatc cacaccaccg atccggaagg gaaaccggtg ccttccaatc
     7861 tttcgatagg gatagtggat gataaactct ggacctttgc cgatgacaaa caggatcata
     7921 ttctttcctg gttattacta agcagtgaac tgaaaggaaa aattgaggaa ccgcagttct
     7981 attttaaaaa agaggaaccc aaagccatcc cggcgctgga cctcctgatg ctcacacatg
     8041 gttaccggta ctttgatttt atcgagtacg tagtcaaaga gggcatgctc aaatacctgc
     8101 cggatcagga taatattgtg agtggccgga tacaggacct gaatggcaac ccggtacagg
     8161 caaaaatgtt cctgatgaat agagtatacg gcgggcgggc tatggaatat actaccggag
     8221 cagatggcgt attcttcttc agtgacctga tttccaacag cagttattac ctgtttgcct
     8281 tgccactaaa aagcactggt aatgtagtaa tcaatattct tcaaaacgga ttgggttata
     8341 atccggcaaa aacccgggcg ctgaatatgc tggccgctaa acccaaagta tttgaaggga
     8401 tcgggatcag ccctcttccg gtagctggca aaaaagaact ggaagcaaaa aaacaggatg
     8461 tgttatttaa caacagacaa tccgccctga atgaagtagt cgtggtgggt tatggagcac
     8521 aacggaaagc aagtttaact gcggccgtat cagtggtcag agcagatgaa atagggacta
     8581 cggcccaact tggcaatatg ctccagggaa aagtagccgg actgcagatc actcaatccg
     8641 gtaattttct gcaaacgcct tcgatacgac tgcgtggaag cagcacggtc accggtaacc
     8701 atgaaccgtt gtttatcctg aacggtgtgc cgatggatgg attctccctg aacacactca
     8761 acccgaatga tatcgataat attaccgtat tgaaagacgg aaacgcgact gccctttatg
     8821 gatacagggc ggcttatggg gccattgtta ttgaatcaaa aaaattaagg agggaaagaa
     8881 tcaagctgaa attcaaaaac cccacttatt ataccagcca gcaattcaat acaagcggca
     8941 ccagttatac accggtacgc agattctatg cgccccttta cgaaacggtc aaaacagaag
     9001 aaaggtccga ttttcgggaa accatttact ggaacccggt ggtacaaaca aataaagaag
     9061 gcaaggctga aattgatttt tataattcgg atgccagcac cacgttccgg gctgtcgcag
     9121 aagggattgg gttcaatggt attcccggta gaaccgaaaa tacctatgcc gtacaaagta
     9181 acctgcggct ggatgccaag attcctcctt atctcacggt aggggacaag gccctgattc
     9241 ccctggtaat caaaaataac agcggacagt ccggcaccgc cgggatagaa gtggtcactt
     9301 ctgcggattt tataacaggt gtgtataccc gaaattattt tcttgagccc gatagttctg
     9361 tacggttgct gattccactg gaagcccgtc gtgccggtca gggacaaata caattcacgg
     9421 tgaagagtga gttcgggaat gaaacccttt ccctgccggt cagtgccgca gagaaaggat
     9481 tcccggtcag cctggttttt tccggtaaca agtccggcca gcatgaattc cccatcagca
     9541 gaatggttcc tggtacgttg cggacaaaac tgcagttgtt ctcttcgatg gaagggcaac
     9601 tgctcgatgg aattgaatcc atgctgcgtg agccctatgg ttgttttgaa caaacctctt
     9661 ccaccacgta tccgaatgtt tttatcctga agtatctgaa ggaatccggc aaatccaatc
     9721 cgaaaattga acggaaagcc atggactata taaaacgtgg ttataatcgt ttgattggat
     9781 ttgaaaccaa acaggatggc tttgaatggt ttggcaatac gccaccgcat gaagcgctca
     9841 ctgcctatgg tttgcttgaa ttcaccgaca tgaaggaatt tatcgatgta gatgaaaaaa
     9901 tgctcacacg cactaaaaac ttcctgctca gtcgccggga tggtaagggc ggatttacta
     9961 tccagaaaaa aggatatgat cagtttgctt ccgtaccgga taaaatcgca acgttgtaca
    10021 taatctatgc actgactgaa gccgggttaa ccaaagaagt ggaaaccgag taccgcgccg
    10081 gagtaaaaaa agcactggag agcaaagaca cttatcaact ggccctgatg gcgctggcgg
    10141 ccaataatat gaatgatcag gaaagttatc agcggctgat gcgcctgctg gccgatcagc
    10201 cggaaaaagg gaaaatggaa acttcggtgg taaattcaag agatgcttcc ctccgggtgg
    10261 agacctggtc actgcaggcc ctggccctgc tgcgggccaa ccaacccgat atgggccgtg
    10321 tggccaatct gctatcaaaa attctcggtg aaaaaagtta ttacggctat ggctccaccc
    10381 aggccaccgt tctcgcctta aaggccatca ccgcctatgg acgcctggcg ggtaaagttg
    10441 ccggggaaac agaaatcgat ttcaccctca acaaagaccg ggtcagccat tatgattcat
    10501 taaacagttt attgcagact ggtaacaata gtttcaatgt agtgtatcgc aaaaatgaaa
    10561 atgcggttcc ttataatctc gaagtttctt acagcacatt tattccgccc tcagctgata
    10621 aagcggaaat cagtctcatt acccacctga aatccggaac cagtctgacc ggtgaaacag
    10681 tccggatgga tattgaagtg acaaacaccc ggtctgtatt gcaacccatg gccattgcca
    10741 agatcggcat accggcaggc ttgtccattc agccctggca gctgaaagag ttcattgaaa
    10801 agaataaatg cgcttattat gagatcttcg ataattacct cgtgttttac tggatgggtt
    10861 ttgcgccgaa tgaaacaaag acattgggac tggacctgaa agccgaaatc cccggaacgt
    10921 ataaagcaaa ggccagcacg g
//
LOCUS       DAOPMW010000075        10310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_6994_length_10310_cov_7.972014, whole genome shotgun sequence.
ACCESSION   DAOPMW010000075 DAOPMW010000000
VERSION     DAOPMW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_10310_cov_7.972014"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1425
                     /locus_tag="PLU11_05630"
     CDS             58..1425
                     /locus_tag="PLU11_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPN58628.1"
                     /translation="MNKIPVIAIFDVGKTNKKLLLFNEQYELVQEDSRPMDEITDEDG
                     FPCDDVQALTAWIRESYSRLLSSQQYEIKGINFSGYGASFVYIGSDGRVMLPLYNYLK
                     PYPKPLLDKFYTKYGGEDFVASQTASPVLGSLNSGMQVYRLKEERPDVFSHVIAALHL
                     PQYLSYVMTGALHTDITSIGCHTNLWDFTQQHYHRWVTEENIELKFPALKDCTELAGY
                     TKEGIPVGTGLHDSSAALIPYLASFTEPFVLLSTGTWCITLNPFNPRPLSISELKSDC
                     LCYLTYKGRPVKASRLFAGNEHEQQVKRLSVHYQKEVDYYKTVQADPLLLRKYLPDFD
                     GLGKDLEGYLPVESVFADRDLADFASYEEAYHLLIADLMVLQVQSSWLVMKDTAVHKI
                     FVDGGFSKNPIYMQLLSAAFPEMEVYAATVAQASSLGAAMAIHSLWNKQSLPASLVQL
                     KMYGH"
     gene            1438..2175
                     /locus_tag="PLU11_05635"
     CDS             1438..2175
                     /locus_tag="PLU11_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPN58629.1"
                     /translation="MKIALFIPCYIDQFYPQVGQATLELLTKLGCEVDFPLNQTCCGQ
                     PMANSGFAAASKDCDKNFVRNFTGYDYIVGPSGSCVLHVKEHLKDDQHSEETIHIRKH
                     IYELTEFITDVLKKEVHNAVFPHKVGLHNSCHGQRGLGLSSMSERMLPAFSKPEQLLS
                     KVKGIQLLKPVRTDECCGFGGTFSVFEEAVSVKMGKDRIKQHEANEVEFITGVDVSCL
                     MHLEGILKRQGSKVKTIHIAEILNTEA"
     gene            2177..3541
                     /locus_tag="PLU11_05640"
     CDS             2177..3541
                     /locus_tag="PLU11_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HPN58630.1"
                     /translation="MKDHATLAEEFIKDDARTNWHDETLWFVRQKRDKAAHGLPEWEQ
                     LREWASQIKNHTLSNMESYLEEFEKNALDNGVKVHWAADATEHNQIIHDIIKSKGITR
                     IVKSKSMLTEECHLNHYLEEQGVEVVDTDLGERIVQFRHEPPSHIVLPAIHLKKQDVS
                     DTFHEHLHTEKGNNDPTYLTRAARVHLREKFIASQLAITGVNFAIAETGGVVVCTNEG
                     NADMGTHAADVHIACMGFEKLIPKASHLSVFLRLLARSATGQPITTYSSHFHKPRAGQ
                     EMHIVIVDNGRSDILGKADFRNALKCIRCAACFNTCPVYRRSGGFSYHTAVAGPIGSI
                     LNPNFDPKKYADLPFASSLCGSCSNVCPVKIDIHDQLWKWRQELSKGGHLPLSKKIMM
                     KGMAFVLGRPALYRFGGASARWVLRHMPFLVKFKVNPWYKQREMPEAPKQSFRDWYIQ
                     NKKK"
     gene            3538..4113
                     /locus_tag="PLU11_05645"
     CDS             3538..4113
                     /locus_tag="PLU11_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPN58631.1"
                     /translation="MSSRDKIFTAIRKNKPESVPYTEPDLSLVINYPDLMSQFSTVLT
                     AIGGSCKEVASATEAKQFIDSIDKGAFVIDTTGVYPSDLTHLSPASLAAVDTAIIRGG
                     VAVAENGSIWVTEENMVNRLLPFIAQQLILVIHEKDLVSTMHQAYERISVNETGFGVF
                     IAGPSKTADIEQSLVIGAHGPCGMMAVVIRN"
     assembly_gap    5143..5242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5948..7069)
                     /locus_tag="PLU11_05650"
     CDS             complement(5948..7069)
                     /locus_tag="PLU11_05650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPN58632.1"
                     /translation="MKNHFHLLQLDLYFTEYKFALLCLVSAFVVTLISIPPIISLVRK
                     YKLYDKPNARKEHTMPIPTMGGIAIIAGMAASLFLWFPFSTEPALISFFFSVIILFGL
                     GIMDDLKDLSAKYKFIIQIALALLIAISGIRITSFGGLFGIEEIPVQAQFTITVLAIV
                     GITNAFNLIDGIDGLAGGLGFMSLITLGLFLIMNRDGNTALIAFALAGGILAFLYFNF
                     NPAKIFMGDTGSLVLGFVVAVLSIRLLQVNGAAINPALPNAPVFITGVALIPVYDTLR
                     VFLVRMWKGNSPFSPDKTHIHHLLTNQGYTHGFASKLICFIHGFILIEIYFLRNMRME
                     FQLLLLIGFMLFVTVMLKRLGKLLGKNERVHEVIKEYLG"
     gene            7250..8425
                     /locus_tag="PLU11_05655"
     CDS             7250..8425
                     /locus_tag="PLU11_05655"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN58633.1"
                     /translation="MTIFHFLFYASFGILFYSYLGYGGLLWLFTRKPAQQKKQTTDEQ
                     WPAVTIIIAAFNEAAILDQKISNTLSIDYPTHRFKILVVTDGSTDESATVLSRYPSVK
                     GIHLPERKGKYAAIRHAMKEVDTPLVVFTDANTLLNPECIKRIVPHYQDPAVGGVAGE
                     KKIRVDAQNSAVGQAEGRYWRYESFMKKMDARFYTVVGAAGELFSIRTALFRPAKEEW
                     ILDDFMMAMHICLQEYKMAYEPGAFATEAPSVSLAEEQKRKVRIAAGAYQAAARLGKA
                     LNIVRHPRLAFQYFSRRILRWFFCPLALVLLLVSNIGLVWQHPDSLFFRVILLLQLFY
                     YLLSLAGWLMLRTGKKAGLLGIPYYFLFMNSCLIKGFILYHRGKQTVLWEKAERQAN"
     gene            8539..>10310
                     /gene="recG"
                     /locus_tag="PLU11_05660"
     CDS             8539..>10310
                     /gene="recG"
                     /locus_tag="PLU11_05660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPN58634.1"
                     /translation="MLKKELNIFTFGDLLDHYPFRHIDRTKLNQVSELTPLSEYAQVA
                     GVLLSFDIIGQKAGKRLVAQLKDKTGILELTWFQGINWVQKLLEPGKSYLVYGRVGFF
                     NGNPQIVHPELEVLVPDQPLAKNFLEPVYSTTEKLKARGLGGRQIGKLTQLLMMQVKE
                     KDVEENLPDEIIKQLKLPGRFKALQQIHFPQSEQDYLEAVKRIKFEELFMAQVRMNLL
                     RSQRHRFSKGVIFEKVGDYFNDCYKNHLPFELTGAQKRVIREIRADTATGKQMNRLLQ
                     GDVGSGKTIVAVLTMLLAVDNGYQACLMAPTEILAQQHYQGIGELLAKVGVSVRLLTG
                     SVKSAERKKILAELASGELKILLGTHAVIEEVVQFKKLGIVIIDEQHRFGVEQRARLW
                     KKAEVPPHMLVMTATPIPRTLAMTAYGDLDYSVIDELPPGRQPITTVHRFESQRSKVM
                     DFIRAEIDKGRQVYIIFPLIEESDKMDYENLMKGYENVKAYFPEPKFWISMVHGRQPA
                     EQKELNMKRFVEHDTQIMVSTTVIEVGVNVPNASVMVIESSEKFGLSQLHQLRGRVGR
                     GAEKSYCVLLTGSKLGNDARERIQ"
BASE COUNT         2740 a         2305 c         2570 g         2595 t
ORIGIN      
        1 gatcagggaa gcactgacta aagaaagagg aacgaatacg atcgcaacag gactctgatg
       61 aacaaaattc cggttatagc catcttcgat gtggggaaga ccaataagaa gctgctgctg
      121 ttcaatgaac agtatgaact cgtgcaggaa gacagccgcc cgatggacga gatcaccgat
      181 gaggatggtt ttccctgtga tgatgtgcag gcactcacgg cctggatccg cgagtcctat
      241 agccggttgc tcagcagtca acaatatgaa ataaagggca tcaacttctc cggctacggg
      301 gccagttttg tctatatagg ttccgatgga cgcgtgatgc tgccgctgta caattacctg
      361 aagccttacc caaagcccct gctcgataag ttttatacaa aatatggggg agaagatttc
      421 gtggccagtc agacggcttc gccggtactt ggcagtctca actccggcat gcaggtctac
      481 cggctgaaag aagaacgccc tgatgttttt tcccatgtaa tagctgccct gcacctgccc
      541 cagtacctca gttatgtaat gaccggtgca ttgcataccg atatcacgag cattggttgt
      601 cataccaatc tctgggactt tacccagcaa cactatcaca gatgggtaac cgaagaaaat
      661 atagagctga agtttcctgc attaaaggat tgtacagaac tggccggtta tacgaaggaa
      721 ggtatccctg tcggaaccgg attacatgat agttccgccg cgttgattcc ctatctggcc
      781 agttttactg agccctttgt gctcttatcc acgggtacct ggtgcattac cctgaatcct
      841 tttaatccaa ggccgcttag catcagcgaa ttgaaaagcg attgtctttg ttatctcacg
      901 tataaaggaa ggcctgttaa agcctcccgt ttatttgcgg gaaatgaaca tgagcaacag
      961 gtaaaacggc tgtcggtcca ttatcagaaa gaagtggact actataaaac cgtacaggca
     1021 gatcccttgt tgctgagaaa gtaccttccg gatttcgacg gattgggaaa agatctggag
     1081 ggatatttgc ccgttgaatc agtatttgcg gatcgcgatc tcgctgattt tgccagctat
     1141 gaagaagctt atcacctgct tatcgccgac ctcatggtat tgcaggtgca gagcagctgg
     1201 ctggtcatga aggatactgc tgtccataaa atatttgtag atgggggttt cagcaagaac
     1261 cccatttata tgcaactctt atcggccgcc ttcccggaaa tggaagtata tgcggccacc
     1321 gtggcccagg ccagttcgct gggagccgct atggcgatcc attcgctttg gaacaaacaa
     1381 tcgcttcctg ccagcctcgt acagctgaaa atgtacggac actgaaacgg atgaatcatg
     1441 aaaatcgcgc tctttatacc ctgttatatt gaccagtttt acccgcaggt cggacaagcc
     1501 acgctggaac tcctgaccaa actcgggtgt gaagtggact tccccctcaa tcagacctgt
     1561 tgcggacagc ccatggccaa cagtggattt gccgctgcgt cgaaggactg tgataaaaat
     1621 tttgtccgca attttaccgg ctatgattat atcgtaggtc cttccggcag ttgtgtgctg
     1681 catgtgaaag agcatttgaa ggatgatcag cactccgagg agaccattca tatccgcaaa
     1741 catatttatg aactgactga gttcatcacg gatgtgttga aaaaagaagt gcataatgcc
     1801 gtgttcccgc ataaagtcgg actgcataac agttgtcacg ggcagcgcgg actcggactc
     1861 tcttccatgt ccgaaagaat gttgccagcg ttctccaaac ccgaacaatt actcagtaaa
     1921 gtaaaaggta ttcaattgct gaagccggtc cggactgatg aatgttgcgg ctttggcggc
     1981 actttctcgg tatttgaaga agccgtgagt gtaaaaatgg gaaaggaccg gatcaaacaa
     2041 catgaagcca atgaagtgga atttattacc ggggtggatg tgagttgcct catgcacctc
     2101 gaaggcattt taaaacgtca gggcagtaaa gtcaaaacca tccatattgc agagatccta
     2161 aacacggagg cctgagatga aagatcacgc tacattagct gaggaattta tcaaggatga
     2221 tgcccgcacc aactggcacg atgaaacttt atggtttgtc cgccagaaaa gggataaagc
     2281 ggcacatgga cttcctgaat gggaacagtt gcgtgaatgg gcttcccaga tcaagaatca
     2341 tacgctctcc aatatggaaa gttacctgga ggagtttgaa aaaaatgcat tggacaatgg
     2401 ggtaaaggtc cattgggccg ccgatgcaac agagcataac cagatcatcc atgacataat
     2461 aaaaagcaag ggcatcaccc ggatcgtcaa gagcaagagt atgctcacgg aggaatgtca
     2521 cctcaaccac tacctcgaag agcaaggagt ggaagtggtg gacacggatc ttggggaacg
     2581 cattgtgcag ttccggcacg agccacccag tcatattgta ttgcccgcta ttcacctgaa
     2641 gaaacaggat gtgagtgata cctttcacga gcacctgcat acggagaagg ggaataatga
     2701 tcccacttat ctcacccgtg ccgcgcgggt acacctgcgg gaaaaattca ttgcttccca
     2761 gttagcgatc accggggtga actttgcgat tgctgaaacc ggtggggtgg tggtctgcac
     2821 caatgaaggc aatgccgata tgggtacgca tgcagccgat gtgcatatag cctgtatggg
     2881 ttttgaaaaa ctgattccga aagccagtca tttatccgtc ttcctacgtt tgctcgcccg
     2941 cagtgcaacg ggacaaccga tcactaccta ttccagtcat ttccataagc cccgggccgg
     3001 acaggaaatg catatcgtga ttgtggacaa tgggcggtct gatattttag gtaaagcaga
     3061 cttccggaat gcattaaagt gcatccgttg tgccgcttgt ttcaatacct gtcctgttta
     3121 ccgccgtagt ggcggcttca gttaccatac tgccgtggcc ggtccgatcg gttccatcct
     3181 caatcccaat ttcgatccga agaaatatgc cgatctgccc tttgcttctt ccttatgtgg
     3241 cagttgcagt aatgtctgtc cggtcaagat cgatatccat gaccagttat ggaaatggcg
     3301 gcaggagctc agcaagggtg gtcatttgcc gctttcaaaa aagatcatga tgaaaggaat
     3361 ggcctttgtg ctggggcgtc cggccctgta tcgtttcggt ggtgcttctg cccgttgggt
     3421 cttgcggcat atgcccttcc tggtgaagtt caaggttaat ccctggtaca agcaacgcga
     3481 gatgccggaa gccccgaagc agagcttccg tgactggtat attcaaaaca aaaagaaatg
     3541 agcagccgcg ataaaatatt tactgcgatc cggaagaaca aaccggagtc cgtgccttac
     3601 acggagccag atttgtcatt ggtcattaac taccctgacc tgatgtccca gttcagcacc
     3661 gtgcttacgg ccattggcgg cagttgcaaa gaagtagctt cagctacaga agcaaaacaa
     3721 ttcattgatt ccattgataa aggtgcattc gtcatcgata ccaccggcgt ttatccatca
     3781 gatcttactc atttatcccc cgcctcactg gccgctgtcg atactgccat tatccgcggt
     3841 ggtgttgccg tagccgaaaa cggttccatc tgggtcaccg aagaaaatat ggtcaaccgc
     3901 ctgcttccct tcatcgccca gcaactcatt ctcgtcattc acgaaaaaga cctggtttcc
     3961 accatgcacc aggcctacga acgcatctcc gtcaatgaaa ccggtttcgg tgtcttcatc
     4021 gccggtcctt ccaagactgc cgatatcgaa caaagcctgg tgatcggggc gcatgggcct
     4081 tgcgggatga tggcggtggt tatcagaaat tagttcgagg ttggaggttg gaggttcgag
     4141 gttggaggtt cgaggttgaa agaagaaaga ggcttaaagg atgggttgaa ggtttataag
     4201 aggttgaaag gagatagaga ttaatggatt ttgtaaaggt taatgatgtt tttctttgta
     4261 ttagccccgt tagggatggc atattactag aagttgaggg tgtatgctat ttctctttgt
     4321 attatccccg ttatggatgg catattacta gaagttgagg gtgtatgcta tttttctttg
     4381 tattatcccc gttaggggcg gcatatcatt agaagtcgag gatgtatgca atttttcttt
     4441 gtattagccc tgttaggggc ggcatattaa tagcaacaag atttagcaag atacccgggg
     4501 agccctgtag gggcgaacta ttgaaaaggt tgagaaaaaa gaaaggcgga aggaagggtt
     4561 aagggttgag cgtaggctgt tgaggattga gtagttttat tttcagtcat tagcgtgagg
     4621 taactaccaa aaattggtct tattggaaaa cctgacttag cgtattaact ttgtagtaaa
     4681 tgttctctga tgacacccgc acaaaactgg aaaatatcat tcgcggagct ctccttgagg
     4741 gcgaggaagg tgctggaact gcagtccgga atttcttatg aggacgcttt aaaacaggtg
     4801 caaaggttaa agcagacttc gaaggtcagt aatgccgcaa agaaatccag tcagtaacaa
     4861 ctgcacttca atttcgagcg tagcgagaaa tcttgccctt cttaagccat atttaagccg
     4921 tcattccgag cgaagcgagg gaccctgtat tttaaaaaca gtcacttcaa aaaaagctag
     4981 ccatatcaat gattgtgatg gagcgacttt gttaattatc agccagcaat caatttgttt
     5041 agcaaaccac cctgtacttt agaactaact ttttaagtaa agtagcttgt tttttgatag
     5101 tcgtgcaaat acaaggtccc tcgcttcgct cgggatgacg tgnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnatttttgt ttttacggca cctgcaaaat acaaggtccc
     5281 tccttcgtcg ggatgacaat atttgggggg agagaaaggg gaaatacaag atttctcgct
     5341 tcgctcgaaa tgtctgtgga tttctttttt ataagcactg gcaaaataca ggatctctcc
     5401 ttcgtcggga tgacgtgatt tttgttttta cggcacctgc aaaatacaag gtccctcctt
     5461 cgtcgggatg acgtgattta tggggagggg aaggggaaat acaagatttc tcgcttcgct
     5521 cgaaatgtct ttggatttct tttttataag cactggcaaa atacagggtc cctccttcgt
     5581 cgggatgacg tgatttatgt gggggagaag gtgaaataca agatttctcg cttcgctcga
     5641 aatgtctttg gatttctttt ttataagcac tggcaaaata cagggtccct ccttcgtcgg
     5701 gatgacgtga tttatgtggg ggagaaggtg aaatacaaga tttctcgctt cgctcgaaat
     5761 tcaagtagca ggctaagttt tttaattttt gtaaaaaaca ctgaaccgtt tcaagggttt
     5821 aatattaaac tccggggtac gctttggtcc ttatttgttt cttgcttctt gattattctt
     5881 tggcttcttg gcgtcttcgg ttcttggtgc ttctttggcg tcttggcatc ttggcttctt
     5941 gatgctttta cccgaggtat tccttaatca cctcatgcac cctctcattc ttccctaaca
     6001 acttaccaag tcgcttcagc atgaccgtta caaacaacat aaacccgatc aacaacaata
     6061 gctgaaactc catccgcata ttccgcagga aatagatttc aatcaggata aatccatgga
     6121 taaaacaaat cagtttggac gcaaatccat gcgtataccc ctggttggtg agcagatgat
     6181 ggatatgcgt tttgtcggga gagaaaggag agttgccctt ccacatccgt acgagaaata
     6241 cacgcagggt gtcgtataca ggaatcaggg ccacgccggt gatgaacacg ggggcattgg
     6301 gcagggccgg attgatagcg gcaccattga cttgtaatag cctgatggaa agtacagcca
     6361 ccacaaagcc caataccagc gatccggtat cgcccatgaa tattttagcc ggattgaaat
     6421 tgaagtacag gaaagccagg ataccaccgg ccagggcaaa agctattaat gccgtattac
     6481 catcccggtt catgatcaga aaaagtccca aagtgatcag gctcataaaa ccgagaccgc
     6541 cggcgagtcc gtcaatgccg tcaattaaat taaatgcatt cgtgatcccc acaatggcaa
     6601 gtactgtaat ggtgaactgc gcctgaactg gaatttcttc aatgccaaag agtccgccga
     6661 aactggtaat ccggatgccg gagatcgcta tcagcagggc cagggctatc tgtatgatga
     6721 atttatattt cgcagagagg tctttcaggt catccatgat gccgaggcca aagaggatga
     6781 ttacggaaaa gaagaaactg attaatgcgg gttctgtgct gaatggaaac cagaggaaca
     6841 gtgaagcggc cataccggcg atgatggcga tacctcccat ggttggaatc ggcatcgtat
     6901 gctccttgcg ggcattgggc ttatcatata atttgtattt ccggaccagg ctgataatag
     6961 gagggatgct gatcagcgtg acgacaaacg cggataccag gcataacaac gcaaacttat
     7021 attcagtgaa atacagatca agctgtagta agtgaaaatg gtttttcatc acaaatggtt
     7081 tgtcctgaat ggtggattta cgtaagtaaa gtaggtagct tttcccattc cagcaaggcc
     7141 ttttaatgca acggtgctgg tatttaccct tttaaagggg ggcggtgcag gatttagcgg
     7201 catatttggg atatttgcga agggtgaacc gtatttttca cattatttca tgaccatatt
     7261 ccatttctta ttttacgcct ctttcgggat cctgttttat tcctacctgg gatatggcgg
     7321 tttgctctgg ctgtttaccc gtaaaccagc acagcaaaag aaacaaacga cggatgaaca
     7381 atggccggcg gttaccatca tcatcgccgc cttcaatgaa gccgctatcc tggatcaaaa
     7441 aatcagcaat accttatcga tcgattatcc cacacaccgt tttaagatac tggtggtgac
     7501 cgatggttcc accgatgagt cagcaaccgt gctgagccgc tacccctccg taaaaggaat
     7561 acatcttcct gaacgtaaag gaaaatacgc cgcgatacgc catgccatga aagaggtgga
     7621 taccccgctg gtggtcttta cagacgccaa tactttattg aatccggaat gtatcaaacg
     7681 aatcgttccg cattatcagg atcccgctgt cggcggagtg gcaggagaga aaaagatcag
     7741 ggttgatgcg cagaattcgg ccgtgggaca ggcagaagga cgctactggc gttacgaatc
     7801 ttttatgaaa aaaatggatg cccgttttta tacagtagtt ggcgccgccg gtgaattgtt
     7861 ttctatccgt accgcattat tcaggccggc gaaagaagaa tggatcctgg atgattttat
     7921 gatggctatg catatttgtc tgcaggaata taaaatggcc tatgaaccag gggcctttgc
     7981 caccgaagcc ccttcggtat cattggccga agaacaaaaa cggaaagtaa ggattgctgc
     8041 cggggcctac caggctgctg cccgcctggg taaggcattg aatatagttc gtcatccccg
     8101 cctggccttt caatactttt cgcgtcgcat cctccgttgg tttttttgtc cgctggcact
     8161 ggttctgctc ctggtcagta atattgggtt agtgtggcaa cacccggact ccctgttttt
     8221 ccgggttatt cttttactcc agctatttta ttacctactc agcctggccg gctggctgat
     8281 gttacgcacc gggaaaaaag caggactgct gggcattcct tattacttcc tgtttatgaa
     8341 cagctgcctg atcaaaggtt ttatattata ccacaggggt aaacaaaccg tactctggga
     8401 aaaagcggag cgacaggcca actgattgcc cccgccattg tttacatttg ccggaaagca
     8461 ttttttattt cagcaggtac ctccatatta gccaatccca tcgaatacct caaaggggta
     8521 ggacccctgc gggccgacct gctcaaaaag gaactgaaca ttttcacgtt cggcgacctg
     8581 ctcgatcatt atcctttccg gcatatcgac cgcaccaaac tcaaccaggt aagtgaactg
     8641 acaccgctca gtgaatatgc gcaggtggca ggggtgctgc tgagttttga tatcattgga
     8701 caaaaggcag gaaaacgact ggtggcccag ttgaaagaca agaccggcat cctcgaactg
     8761 acctggtttc aggggatcaa ctgggtacaa aaattactgg aacccggaaa gtcttacctc
     8821 gtctatgggc gcgttggatt tttcaacggc aatccgcaga ttgtacatcc ggaactggaa
     8881 gtattggtcc cggatcagcc tctggcaaaa aacttccttg aacctgttta ttccactacg
     8941 gaaaaattaa aagcccgggg actgggtggc cgtcagatcg gcaaactcac ccagttactg
     9001 atgatgcagg taaaggaaaa agatgtggaa gaaaacctgc cggatgaaat aatcaaacaa
     9061 cttaagctgc ccggtcgctt taaagccctc cagcagattc attttcctca atcagagcag
     9121 gactacctgg aagcagtgaa acgcatcaaa tttgaagaac tttttatggc ccaggtgcgc
     9181 atgaacctgc tccggagtca gcgccaccgt ttttcaaagg gagtgatctt tgaaaaagtg
     9241 ggagattatt ttaatgactg ttataaaaat caccttccat tcgaactcac cggggcacaa
     9301 aaaagagtga tacgggagat cagagccgac acggccacgg gcaaacaaat gaaccggctc
     9361 ctgcaagggg atgtgggcag tggcaaaacc atcgtggctg tgctgaccat gctgctggcc
     9421 gtggataatg gttaccaggc ctgcctgatg gcgcctactg aaatattagc ccagcaacat
     9481 taccagggta tcggggaact gctggcgaaa gtgggggtgt cagtccgcct gcttaccggt
     9541 tccgtaaaat cagccgaacg taaaaaaata ctggccgaac tcgccagcgg ggaattaaaa
     9601 atactcctgg gcacccatgc cgtgattgaa gaggtggtac agtttaaaaa actggggatc
     9661 gtgatcattg atgaacagca tcgttttggg gtggaacaac gggcccgctt atggaagaag
     9721 gccgaagtgc ccccacacat gctcgtgatg acggctaccc cgattccccg taccctggcc
     9781 atgacggcct acggggacct ggattatagc gtgattgatg aattaccccc cggccggcaa
     9841 ccgatcacca cggtccaccg ttttgaatca cagcgcagta aggtgatgga ttttatccgg
     9901 gccgagatcg ataagggccg gcaggtctat attatttttc ccctgatcga ggaaagtgat
     9961 aaaatggatt acgaaaacct gatgaagggg tacgagaatg taaaagccta tttcccggag
    10021 cccaaattct ggatcagtat ggtgcatggt cgtcagccgg ccgagcagaa agaactaaat
    10081 atgaaaagat ttgttgaaca tgatacacag atcatggtgt caacgacggt gatagaagtg
    10141 ggggtgaatg tgcccaatgc ctcggtaatg gtgattgaaa gttcggaaaa atttggttta
    10201 tcgcagctcc atcagttgcg gggccgggta ggccggggag ctgaaaaaag ctattgtgta
    10261 ttattaaccg ggtcaaaatt aggtaatgat gcccgcgagc gcatccagat
//
LOCUS       DAOPMW010000076        10277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7026_length_10277_cov_7.343181, whole genome shotgun sequence.
ACCESSION   DAOPMW010000076 DAOPMW010000000
VERSION     DAOPMW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7026_length_10277_cov_7.343181"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PLU11_05665"
     CDS             <1..1459
                     /locus_tag="PLU11_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPN58635.1"
                     /translation="YTNDLHAHLDPHRVPWISNTRLVGGFANIATRVKQEKAANPNTL
                     YFDAGDFFTGPFISSLTKGEAVINVMNYLSIDAACIGNHEFDHGWENVPDQLRKARFP
                     VLNGNIFLKGKKKLLWNNPYTIIQMNGIRIGVIGLHGQFAFYDTIADEMIQGVETRDE
                     EKYLRKYIKELRKKADLVILLVHQGIPGRQSSTGNTDVARNLQRDIDLAATIPGIDIM
                     ITGHAHQGTPQALLSNGTIIVSTDALGTELGKLEISYNPLKDKITAHNNTLNYLYDDE
                     VADDTATARVIADWKQKVKQITDEKVTTIPVSLTRSYGEESVMGNMVADAILYTYPGY
                     DMAVTNSGGLRQDIDAGTVTVGNIISAFPFPNTIHQLEIRGVDLYELFEHGAGLTNGI
                     LQVSKGVEIQCDENKPVGSRIISCKLNGMDIDRDKTYKVLTSNFLSDGGDGFVAFKKA
                     LTRKKTTQEIVQSMISYLKQYKEYQPLLEGRIKKI"
     gene            complement(1456..1980)
                     /locus_tag="PLU11_05670"
     CDS             complement(1456..1980)
                     /locus_tag="PLU11_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HPN58636.1"
                     /translation="MSEPFVNKVADSGLITLDLEKWYPREETAVFDLKDHLFMGMILK
                     EKDFREALKAMDWTAYQGKNVALTCSVDAIIPVWAYMLVAANLQPFAKEIVMGEEKEL
                     HKTIFLKNLSKIDVQEFEDKRVVIKGCGETPIADYVYMEITKLLRPVAKSIMYGEPCS
                     TVPVFKKPATAKTD"
     gene            complement(2096..4249)
                     /locus_tag="PLU11_05675"
     CDS             complement(2096..4249)
                     /locus_tag="PLU11_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPN58637.1"
                     /translation="MSIIQDIRDKYAKLTVVLIALALVGFILTDYFAGKSRGMVGGPS
                     NTVGVVNGKKILFDDFSKKVTQAEESMKAQGYPAAAASQQALDQAWTQEINQALLSDE
                     FERLGIEVGKKELGDILYGQNAPQDIKQQFTDEKTGMFNPVLAKQNIDQKLKKGTEEE
                     KANINAYIGQLINLRMADKYFSLMTGTTNYPKWMIEKQNADNAQMARISLVRETYASV
                     PDSSIKVEDSEIDAFVSKNKSLFKQNESRSISFVSFSAEPNAKDSAATLNSVLTLRDR
                     FSATDSIAQYLAGEGAQYFDGYVSGNAIQIPVKDSILKLPVGGVFGPYLDGNSYVLAR
                     MIGMRVLPDSIKCRHVLVSNNPQQGGVDDSTASRRIDSVKLAINSGANWDSMVQKYNP
                     VSDGSRQNKGEMTFSSTQIQDVNFAKEFAQFILFDGKVGERKVVKTSFGYHFIEIMSA
                     IKPSTHYKVAYLPKEIIASQETDNAALNEANSFAGESTDIKSFDDNYEKKLKTKGFQK
                     GIATDIARSSSSIRGLESASRSFVKDIYDAKRGEVLKPVRVDNSYVVAVVTEALEEGT
                     MSTTRARAAGAESEVRTKKKGELLKKKIGTVTTLEDAIAKLGGVKMIEPVDSLRMTGG
                     SAISYEPKVIGAAFNPSNKGKVVPEVIVGKQGVYVVRVEDQTATAVAAEDINAVRNAR
                     YLQAKQSMGNQYSPNNPLSILRNAADIKDKRSERY"
     gene            complement(4445..6616)
                     /locus_tag="PLU11_05680"
     CDS             complement(4445..6616)
                     /locus_tag="PLU11_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPN58638.1"
                     /translation="MKSIKRSLVAFLLFLVITARADEGMWLPLFLQQLNEKQMKSMGM
                     KISASDIYNINKGSLKDAIVSFGGFCTGEVISEQGLVLTNHHCGFDAIQNHSTLDRNY
                     IRDGFWARNKGEEIPNPGLFVTFIVRIEEVTKSALEGVKPTMTEQERQSTIDKNIAAL
                     RSGVKKEAYQDYFVRSFFEGNQYFMFVTETYKDVRLVGAPPSAIGNFGKDTDNWMWPR
                     HTGDFSMFRIYAGKDNKPAEFSENNVPYKPKKALSISLDGMKEGDFTLVFGFPGRTTQ
                     YLHSAAVEQIVQVNDPAKIAIRDKALGVIDGFMRRDEQIKIQYAAKYASISNSWKKWQ
                     GEVLGLTSTNAVAKKKANEAEFQKRVNANPAFKEQYGTLLGDLESAYQELNKYGLARD
                     YYTETLSRIEAFAIGSRINSLAGIKQKNGDKAFKDAVPKTIEILGELYKEYSPTVDKK
                     LFEVLMEKFTKDQPEEFLAPELVTLTKTYQSDFAKMADDFYSNVDLLNNGAKVLANLQ
                     ANPDLVLMANDLNKANQLLLQLNSHYASSVTKKLSGIQARVNQLQRTYMKAQMEVMKE
                     KKFYPDANSTLRVTYGNVKGYQPRDAVKFDFYTYLDGVMDKYKPGDYEFDVPEKLRIL
                     YKNKDFGQYGKNGKMPVCFIAMNHTTGGNSGSPALDANGNLVGLNFDRAWEGTMSDIN
                     YDPSICRNIMVDIRYVLFIVDKYAGAGHLVQEMKLVRAGKK"
     gene            7248..8258
                     /gene="nadA"
                     /locus_tag="PLU11_05685"
     CDS             7248..8258
                     /gene="nadA"
                     /locus_tag="PLU11_05685"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPN58639.1"
                     /translation="MNTVLTDTAIANVEKTGFLDIELDPSLDLFAEIEKLKKEKNAVL
                     LAHYYQEPDIQDVADYIGDSLGLAQQAEKTNADMIVFAGVHFMAETAKILNPGKKVVI
                     PDFKAGCSLSDSCPPPLFKKFKEQHPDHIVVSYINCSAGIKALSDVIVTSSNARVIVE
                     SFPKEQKIIFAPDKNLGAYINKVTGRNMLLWNGACMVHEIFSLEKITKLKTRHPKAKF
                     IAHPECEEPVLRLADYIGSTTGLLKYTETDDAMEYIVATETGILHQMMKASSQKTFIP
                     APPTNNCACNDCPHMKLNTLEKLYLCMKYELPEINMDEELRLAALKPIKRMLEISSQA
                     GL"
     gene            8274..9206
                     /locus_tag="PLU11_05690"
     CDS             8274..9206
                     /locus_tag="PLU11_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58640.1"
                     /translation="MKKLLFLLSFFIGLTAAGQSVKELITRGQQLLGEKKFTEALDTY
                     NKAFKKDSTKYDVFTGRGTAYFELKEFQLCFDDFSRAIALEPDSALAYHYRANLMYVL
                     NYTDEAIADNTRAIERTSDEKFLLGCFINRGSAKEQKRDFKGAYEDFYRAYSYDTTDI
                     SVLNNLGTVLDELDRREEGIMYLKKAIALDSNFVGPYVNIGFQYTQMGRYEEALVYFN
                     KALELDKDEPLTLNNRGYTRYKLNNLAGALEDIDKSIILYATNAYAYKNRALVYIALD
                     KKEEACKDLQKARELQFEKQYGPEVNELIKKNCQ"
     gene            complement(9209..>10277)
                     /locus_tag="PLU11_05695"
     CDS             complement(9209..>10277)
                     /locus_tag="PLU11_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58641.1"
                     /translation="SRRQLRSLFSLKWLALILLFVAGISPVLYCYYLQFNLHPEKLVR
                     GKDHIDGVRFILFNQSTERYTGVMGDTLKQDYFFFIHTFLWAFAPWSFLSFYVFYKIV
                     RGIKEQKTEIASALVFIFFIGLVSFSGFKLPHYLNSGFPFAAVFTAGFLLRSANNLLL
                     VKRIYQLQLILTLLILLAVAILNFWSFQVNSYFILTGVILLLSVYFHFIRSSLMHTLA
                     KSISLSVTALVLSFFLLNTNFYPQVLNYQGGNKLAAAIKGKADPRDIYFWEGSNSASF
                     DFYTKTLRKGYLGREGKAGKKVWLVYYPKDTLAIKEKGIKLANTIRIKDYEITKMDAA
                     FLNPGKRDSVCSVIMMSEILP"
BASE COUNT         2883 a         2309 c         2188 g         2897 t
ORIGIN      
        1 atataccaat gacctccacg cccatctcga cccgcaccgg gttccctgga taagtaatac
       61 ccgattggtc ggtggttttg ctaatattgc cacccgggta aaacaggaaa aagctgccaa
      121 tccaaatacg ctctattttg atgccggtga tttttttacc ggtcccttta tcagctcatt
      181 gaccaaggga gaggcggtga tcaatgttat gaattacctg tcaatcgatg ccgcctgtat
      241 aggaaatcat gaatttgatc atggctggga gaatgtacct gatcaactgc gtaaagcccg
      301 gtttccggta cttaacggga atatctttct caaaggaaag aaaaaattgc tctggaataa
      361 tccctatacc attattcaga tgaatggtat tcgcatcgga gttattggtc tccatggtca
      421 atttgctttc tatgatacta tagccgatga aatgatccag ggggtggaaa cccgggatga
      481 ggagaaatat ttaagaaaat atataaaaga gctgcgcaaa aaagcagatc tcgttatcct
      541 gttggttcat cagggaatac ctggtcgtca atcctccaca ggaaatacag atgtagcccg
      601 taacctgcaa agggatattg atcttgccgc cactatacct ggtatagata ttatgattac
      661 cggtcatgcc caccagggta ccccgcaagc cttgctttcg aacggaacga taattgtttc
      721 tacggatgct ttaggtaccg aactgggaaa acttgaaata agttataacc ccctgaagga
      781 taaaatcact gcccataaca acacccttaa ttacctgtat gatgacgaag tagccgatga
      841 tacggcaaca gccagggtga tagctgattg gaaacaaaaa gtaaaacaga tcacggacga
      901 aaaagtaact actatacccg tgtcgcttac ccgttcttat ggtgaagaat cagtcatggg
      961 taatatggtt gcggatgcca tcttgtatac ttatcccggt tatgatatgg ctgtaacaaa
     1021 cagtgggggc ttgcgtcagg atatcgatgc aggtactgta acggttggaa acattatctc
     1081 tgcttttcct tttccaaata ccatacatca gttagaaatc aggggggttg atttatatga
     1141 actatttgaa catggtgccg gacttacgaa tggtatatta caggtatcaa aaggagttga
     1201 aattcaatgt gatgaaaaca aaccggttgg ttcccgtatt ataagctgta aactgaatgg
     1261 tatggatatc gatcgggata aaacctataa agtacttact tccaattttc tgtctgatgg
     1321 aggagatggg ttcgtagctt ttaagaaagc cctgaccagg aaaaaaacca cccaggagat
     1381 tgtacaatca atgatcagtt acctgaagca atataaagaa tatcaaccct tattagaagg
     1441 aagaataaaa aagatttaat ctgtctttgc tgtggccggt tttttaaaca ccggcaccgt
     1501 actgcagggc tcgccataca tgatgctttt agcaacgggg cgaaggagtt tagtgatctc
     1561 catatagaca taatccgcga tgggcgtttc gccacagcct ttaatcacca cgcgtttatc
     1621 ctcgaattcc tgcacatcaa ttttggaaag atttttcagg aaaatagttt tatgtaattc
     1681 cttctcctcc cccatcacaa tttctttggc aaagggctgc agattcgccg caaccagcat
     1741 ataggcccaa acaggaatga tggcatctac actacaggta agcgccacat ttttgccctg
     1801 ataagccgtc cagtccatcg ctttcagcgc ttcccggaaa tccttttctt tcaggatcat
     1861 gcccataaac aggtggtcct tcagatcaaa tacggcagtc tcctccctcg gataccattt
     1921 ctcgagatcc agggtgatca gtccgctatc ggccacttta tttacaaaag gttcgctcat
     1981 gtgctttgga tatgtaaaaa taacaaattg ccggaccgga tggttgcgag ttttggaaaa
     2041 caaaaaaccc cggaagttag gcctccgggg tttcattata agttattgag ttttcttagt
     2101 agcgctcaga acgtttatcc ttaatatctg ctgcatttcg caggatagaa agcggattgt
     2161 tcgggctata ctggttaccc atgctttgct tggcctgcag gtaacgggca ttgcgcacag
     2221 cattaatgtc ttctgctgcc acagctgttg cagtttgatc ttccacacga accacgtaca
     2281 ccccttgttt tccaacaatc acttcaggaa ccacttttcc tttattggaa ggattgaaag
     2341 cggcaccaat cactttgggc tcgtaagaaa tagcagaacc accagtcatc cggaggctat
     2401 ccacgggttc aatcattttg actccaccca gtttagcaat agcgtcttcc agtgttgtaa
     2461 ctgtaccgat tttctttttc aggagttccc ctttcttttt ggtacgtact tctgattctg
     2521 caccggcagc acgggcacgg gtagtactca tggtaccttc ttcgagggct tctgtaacta
     2581 cagcaaccac atagctgtta tccacacgaa ccggcttcag gacttcccct cttttcgcgt
     2641 cataaatatc ttttacaaaa gaacgggaag cgctttccag tccacggata gaagaactgg
     2701 aacgggcgat atccgtagca attcctttct ggaatccttt tgttttcagt ttcttttcat
     2761 aattatcatc aaagcttttg atgtcagtac tttcgcctgc aaacgaattg gcttcattca
     2821 gggccgcatt atctgtttcc tggctggcaa tgatttcttt gggcaggtag gcaactttgt
     2881 agtgcgtaga cggtttgatc gcagacatga tctcgataaa atgataacca aatgaagtct
     2941 tcaccacttt gcgttcaccc actttaccat caaataaaat gaactgggca aattctttgg
     3001 caaagttcac gtcctggatc tgtgtggaag agaaggtcat ttcgcctttg ttctggcggc
     3061 ttccgtcact taccggattg tatttctgta ccatggagtc ccagttggcg ccactgttaa
     3121 tcgctaattt aacgctgtcg attctgcggg aagcggtact gtcatccaca ccaccttgct
     3181 ggggattgtt gctgaccaat acgtggcggc atttaatgga atcaggtaat actctcatac
     3241 ctatcatcct ggctaacaca taactgtttc catcaaggta aggaccaaac acaccaccta
     3301 ccggtaattt taaaatagaa tccttaacgg gaatctgaat ggcattgcca cttacatacc
     3361 cgtcaaaata ctgagctcct tctccggcaa gatattgagc aatactatcg gttgcagaga
     3421 aacgatcacg aagtgtcagc acactgttca gggtggccgc actgtctttg gcattcggct
     3481 cagcactgaa acttacaaaa ctgatgctgc ggctttcatt ttgcttgaac aggcttttat
     3541 tcttacttac aaaagcatcg atctcgctgt cttctacttt aatactgctg tcaggaacac
     3601 tggcataggt ttctcttacc aatgaaatac gggccatctg tgcgttatcc gcattttgtt
     3661 tttcaatcat ccacttgggg taattggtcg tacctgtcat cagggagaaa tatttatcag
     3721 ccatgcgcag gttgatcagc tgtccgatat aggcattgat atttgccttt tcttcttctg
     3781 ttcctttttt cagtttctga tcaatatttt gtttggccag caccgggttg aacatgccgg
     3841 ttttttcatc ggtaaactgt tgtttgatat cctgcggtgc attttgtccg tataagatat
     3901 cacccagttc ttttttacct acttcaattc cgagtctttc aaactcatca ctcagtaagg
     3961 cctggttgat ctcctgtgtc caggcctgat ccaatgcctg ctgactggca gcagcagccg
     4021 gatagccctg tgctttcata ctctcttctg cctgcgttac ttttttactg aaatcatcaa
     4081 acagaatttt tttgccattt acgacaccaa cggtattcga tggaccaccc accatgcccc
     4141 ttgatttacc ggcaaaataa tcggtcagaa tgaaccctac cagggccagt gcgatcaaca
     4201 ctacagtaag tttggcgtac ttgtctcgga tatcctggat aatagacata atatattatt
     4261 actgattttt ttaaggatgg caaaaatagg ggaaaaactg tggaaaatct ataaaaagtg
     4321 ctaccgtttt ggaggtgaaa gtagacgaaa aaacgggtat gggggctgat ttcgggcaaa
     4381 taaaacgggc tgacccgttt ttagcgaatc agcccgtttt aaagggaaac ccatttgcct
     4441 gtttctattt tttcccagcc ctgaccagtt tcatttcctg gaccaggtga ccggctcctg
     4501 cgtatttgtc gacgataaac aacacataac gaatatctac cataatattc cggcaaatac
     4561 tggggtcgta gttgatatca ctcatcgtac cctcccaggc gcggtcaaaa ttgagaccta
     4621 ccaggttacc gttggcatcc agggccgggc ttccggaatt tccaccagta gtgtggttca
     4681 tcgcgataaa gcaaaccggc attttaccgt ttttaccata ttggccaaaa tctttgtttt
     4741 tatataagat ccggagtttt tctggtacat caaattcata atctcctggt ttgtatttat
     4801 ccataacccc atccagataa gtgtagaaat caaatttcac ggcatctctc ggctgatatc
     4861 cctttacatt cccataagta acccggaggg tgctgttggc atcgggatag aatttctttt
     4921 ccttcatcac ctccatctgg gccttcatat aggtacgctg caattggttg acccgggcct
     4981 gaataccact caacttcttc gttacactgg atgcatagtg gctgttcaac tgtaaaagca
     5041 actggttagc cttattcagg tcattagcca tcagcaccag atccggattt gcctgcaggt
     5101 tagccaatac tttagccccg ttattcagca ggtcgacatt gctgtaaaaa tcatccgcca
     5161 tttttgcaaa atcggattgg taggtcttgg tgagtgtaac cagttccggc gccaggaact
     5221 cttccggctg atctttggta aatttctcca tcagcacttc aaagagtttc ttatccaccg
     5281 ttggactata ttctttatag agttcgccca atatttcgat ggtcttgggt actgcatcct
     5341 taaatgcttt atccccgttt ttttgtttga tgccggccag gctgtttatt ctggaaccaa
     5401 tggcaaaagc ttcaatgcgg ctgagagttt ctgtataata atccctggcc agcccatatt
     5461 tattcagttc ctggtaggca gattcaagat caccgagcag ggtgccatac tgttctttaa
     5521 aggctggatt ggcattgact cttttctgaa attcagcttc attggccttt ttctttgcta
     5581 ccgcattggt actggtcaaa cccagtactt ccccctgcca tttcttccag gagttactga
     5641 tactggcata ttttgcggca tattgaatct tgatctgctc atcccgacgc ataaacccat
     5701 caatcacgcc aagggctttg tcacggatcg caattttagc cgggtcattt acctgtacaa
     5761 tctgctccac tgcggcagaa tgcaggtatt gggtggtacg gccgggaaaa ccgaagacaa
     5821 gcgtaaaatc gccttctttc atgccatcca gggagataga aagggctttt ttaggcttgt
     5881 aaggcacatt attctctgaa aattctgcgg gtttattatc cttaccggca taaatccgga
     5941 acatggaaaa atcaccggta tgccggggcc acatccagtt atcagtatcc tttccaaaat
     6001 taccgatggc ggatggcggt gctcctacaa gacggacatc cttatacgtc tcagtaacaa
     6061 acatgaaata ctggtttcct tcaaaaaatg accggacaaa atagtcctgg tacgcttctt
     6121 tcttcacccc ggagcgtaaa gcggctatat ttttatcaat ggttgattga cgttcctgtt
     6181 cggtcattgt aggttttact ccctcaagag cagatttcgt tacttcttca atccgcacaa
     6241 taaaggttac aaacagcccg ggattcggga tttcctcccc cttattacgg gcccagaaac
     6301 catcccttat ataattacga tccagggtag agtggttctg gatggcatca aagccgcaat
     6361 ggtggttggt tagtaccaat ccctgctccg aaatcacttc cccggtacaa aaaccgccaa
     6421 aactcacaat ggcatctttc agacttcctt tgttaatatt atatatatca ctggcggata
     6481 tcttcatccc catggatttc atctgctttt cgttcagttg ctggagaaat aagggaagcc
     6541 acataccttc atcggcacgg gcagtaataa ccaggaaaag caaaaaggcg acaagacttc
     6601 gttttatgga tttcatcgtt aatatattta aagaagtaaa attagttcat tcattcatac
     6661 gggaaattcc gctcatcgaa ttagtaaacg ggactgattt ttgcacaatt gaaaataatt
     6721 gggtgcttcc ggatgatcca ggtatgcagg ataaccggaa ttttctccac atcgcactgt
     6781 gcatgggtga aaaattatgt gcataaccgg ctgtggaacg ttaacaggaa aggggacgaa
     6841 tggctttaaa attcacacct ggcttaccgg atctgaaacg atgtccataa atgcacattt
     6901 gttctacaac tatccacaga taaaatttaa aaaggatccg gatagtttta cgcctaaaat
     6961 aatgcacaag tatgtggaaa tctgtgtcag aaccgcgcca gtactgaatc cggcattgtg
     7021 aattaccgta actcaaatgt gtataacagg gaaattgtaa atttgtatcc aataaaacaa
     7081 acgagttttc cacgaatcca cagctgtaat aataatatac tcttttttat aaaaatatat
     7141 taatactatt attgtattaa tcgccaggtt aaaaaatttt gttttgaaaa attgcacggg
     7201 ctggtggaat attgcaccct gatcgaacaa acaaaaccga aagtctcatg aacacagttt
     7261 taacggacac cgcgattgcc aatgttgaaa aaacgggatt tctcgatatc gagctggatc
     7321 cttccctgga tttgtttgca gaaatcgaaa aactcaaaaa agaaaaaaat gcggtgctcc
     7381 tggcgcacta ctaccaggaa ccggatatcc aggatgtggc ggattatatc ggcgacagtc
     7441 tcggacttgc tcaacaggcg gaaaagacca atgccgacat gatcgttttt gccggcgtac
     7501 atttcatggc ggaaacggcc aagatcctca acccgggcaa aaaagtggtg attccggatt
     7561 ttaaagccgg atgttccctg agtgacagct gcccacctcc ccttttcaaa aaattcaaag
     7621 agcagcaccc ggatcatatc gtcgtgagct acatcaactg cagcgcggga atcaaggccc
     7681 tgagtgatgt gatcgtgacc agcagcaatg cccgggtgat cgtggaaagt tttccaaaag
     7741 agcagaaaat tatttttgcg ccggataaaa acctgggggc gtatatcaac aaagtcacgg
     7801 gaagaaatat gctcctctgg aacggagcct gtatggttca tgaaattttc agcctggaga
     7861 agatcactaa actgaaaacc cgtcacccaa aagcaaaatt tatagcccat ccggaatgtg
     7921 aggaacccgt actgcgcctg gccgattata ttggctccac cacgggcttg ttgaagtata
     7981 ccgagaccga tgatgccatg gaatatatcg tggctaccga aacgggaata cttcaccaga
     8041 tgatgaaggc ttcttctcaa aagaccttta ttccggctcc ccccaccaat aactgtgcat
     8101 gtaatgattg cccgcatatg aaactgaata cgctcgagaa attatatctc tgtatgaagt
     8161 atgagttgcc ggaaatcaat atggacgaag aattaaggct ggccgcattg aagccaatta
     8221 aacgaatgct ggaaatcagc agccaggccg ggctttaacg ctaatgctcc gctatgaaaa
     8281 aactcctttt tttactttcc ttttttattg gcctgaccgc tgccggtcag tcagtcaaag
     8341 aactgatcac ccggggtcag cagttactgg gagaaaagaa gtttaccgaa gccctcgata
     8401 cgtacaataa agcctttaaa aaagattcca caaagtatga tgtctttacc ggccggggta
     8461 ctgcttattt tgaattaaag gaattccagc tttgttttga tgatttttca agagccatag
     8521 ccctggaacc cgattcagcc ttagcctatc attaccgggc taacctgatg tatgtactga
     8581 attataccga tgaggccatt gcggataata cccgggctat tgaacggacc tcggatgaaa
     8641 aattcctgct tggctgtttt attaaccggg gcagtgccaa agaacaaaaa agggatttca
     8701 aaggagcata tgaagacttt taccgggctt acagttatga taccaccgat atttctgtac
     8761 tgaataacct tggtaccgtg ctcgatgaac tggaccgccg ggaagaaggt ataatgtatc
     8821 ttaaaaaagc cattgcgctg gatagtaatt ttgtaggtcc ttatgtgaat atcggatttc
     8881 aatacaccca gatggggcgg tatgaggaag cgctggttta ctttaataaa gcacttgaac
     8941 tggataaaga tgaacccctc acgctcaata accgcggtta tacccgctat aaattaaata
     9001 acctggcggg tgcgctggag gatatcgata aatccattat tctgtacgcc accaatgcct
     9061 atgcttacaa gaacagggcg ttggtctaca ttgcccttga taaaaaagag gaagcctgta
     9121 aggacctgca gaaggccagg gagctgcagt ttgaaaaaca atatggtcct gaagtaaatg
     9181 aattgattaa gaaaaactgc cagtaagatc aggggagaat ttcggacatc atgattaccg
     9241 agcagacact atctcttttt cccggattca ggaaggctgc atccatttta gtgatttcat
     9301 aatctttaat gcggatcgta ttcgccagct ttattccttt ttctttgatc gccagggtat
     9361 ctttcgggta atacacgagc catacttttt ttccggcttt cccttcccgg cccagatagc
     9421 ctttgcgtaa tgtttttgta taaaaatcaa acgaagcgct gttgcttcct tcccagaaat
     9481 aaatatccct tggatctgct tttcctttta tggctgcggc cagcttgttt cctccctggt
     9541 aattcagcac ctgcgggtaa aaattagtat tcagtaaaaa gaaagacaat accaaggctg
     9601 ttacagaaag gctgatggat ttcgccaggg tatgcatcag gctgctgcgg ataaaatgga
     9661 aatacacact taacaacaga atgactcctg taagaatgaa ataactattg acctggaagg
     9721 accagaaatt caggatggct acggccagta atattaataa agtcaggatc agttgtaact
     9781 ggtagattct ttttaccagt aaaagattat ttgcagatcg cagcaggaaa cccgctgtaa
     9841 acactgcagc aaaaggaaaa ccactgttca ggtaatgggg cagtttaaaa ccggagaaac
     9901 tgaccagccc tataaagaaa atgaatacca gggcactggc gatttctgtt ttttgttctt
     9961 ttattcctct tactattttg taaaaaacat aaaaagaaag gaaggaccag ggagcaaagg
    10021 cccataggaa tgtatgtata aaaaagaaat agtcctgttt cagggtatca cccatcacac
    10081 cggtataccg ttccgtactc tggttgaata aaataaaacg aaccccgtca atatgatctt
    10141 ttccgcggac taatttttcc gggtgcaggt taaactgcag gtaataacaa taaagtaccg
    10201 ggctgatacc ggcaacaaac aataatataa gcgccaacca tttcagggag aaaagtgatc
    10261 gcagctgccg gcggcta
//
LOCUS       DAOPMW010000077        10256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7056_length_10256_cov_7.140967, whole genome shotgun sequence.
ACCESSION   DAOPMW010000077 DAOPMW010000000
VERSION     DAOPMW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_10256_cov_7.140967"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLU11_05700"
     CDS             complement(<1..232)
                     /locus_tag="PLU11_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58642.1"
                     /translation="MKTKLLLFLVFTCFSINGFSQKPEELLQQWYQKAPVEKIWLHCD
                     RDNYVAGETAWFKAYFYSDFQPDTLSSIVYVEL"
     gene            385..795
                     /locus_tag="PLU11_05705"
     CDS             385..795
                     /locus_tag="PLU11_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58643.1"
                     /translation="MKRLIMACSLLLLLTGCKKAIENAQDDLVIRAMTDGEWVISSFT
                     VNGTPTTPDYSLYKFKYYSNRTVDAIKSGTVEKTGTWDGNTSTMIISANFTAATYPLN
                     QINGSWLITRNSWTYVEATQTIGSDVKTLRLDKQ"
     gene            964..5319
                     /locus_tag="PLU11_05710"
     CDS             964..5319
                     /locus_tag="PLU11_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58644.1"
                     /translation="MKRLISHIHSGRFLLLCLFFYSSVANAQPVANFTASPLAGCSPL
                     IVNFQDLSTGTPTNWLWNFGNGNTSTLQNPIASYFTPGTYTITLTVTNAGGSNTLVRT
                     NYITVYEPPTVDFSASITTGCFPLRVQLTDLSTAGVGNTNVSWDWDFGNGQTSNLQNP
                     VVTYTTAGIFNVTLRVTNDKGCVRTLSRPAFINVTPGVRSLFTHTVPTVCSAPATIDF
                     TNNSTGPPVLSYLWDFGDGNTSTAQNPSHTYVTNGTYTATLITFSSAGCQDTLRSNPI
                     VVGGITTSFSGPVSGCVNEQLSFTVTSNPVPNISNWTFGDGATGVGLNVTHSYAAAGV
                     YPIKLYNTYTNCVDSFIQNITINPLPVVDFTAPVTVSCQPPLTVNFQDLTTGGATAWQ
                     WDFGDGNTSAVQNPSHTYTSYGNFTVTLIATNGFGCTDTIVKPGFIRIRRALMSVTGL
                     PERGCIPYTITPVPVINSLDAVTSWEWDFGDGGTAFVQNPSHTYTVQGTYTVKLIITT
                     SSGCRDTLTLNSAVQVGSKPMANFSATPNPVCARQPVQFTDLSVPADEWLWDFGDGST
                     SILQNPIHNFNDTGYFDITLIAYNNGCPDTIVFTDYIYVLPPIARVIIGPDCNNRLRF
                     SFIDFSVGALTWEWDFGDGSPFDNTQNPVHTYPALGNYSVRLVVTNGGCADTTVVPVY
                     AIDERADFTASQTTLCKIGTVTFNTLNVNPANITSYAWNFGDGNSTTTSTPFVTHTYT
                     NVGTYSVSLTITDLNGCTSTVIKTNYIRINGPTANFIAGNASGCTGLVATFTDQSTTD
                     GQNAITNWFWDFGDGNTQNYTAPPFQHTYNSVGSFNVKLIVTDAAGCRDSLTLISNVT
                     TSDPTPDFVVDTLSCPGAVLTFTNNSLPAGVSSAWDFGDGNTSIVTSPTHSYAATGLY
                     TVKLVIRDALGCSDSLIRTNYIRVALPVADFTMSDSVTSCTPLEIQFTNTSTFYSSVS
                     WNFGTGQGISTLNNPIHFFSSPGIYPVKLIVTSPGGCLDSIIKTVRVFDTAGSRINYT
                     PIGGCKPLSIAMSAITAGPMVSYFWDFGDGNTLTTTTPSANHIYTSFGSFVPRVILED
                     PSGCIIPLQGADTLLVSGATANFGVDTSFFCDYGTVNFIDSTTFNDPIVQYSWDFGDG
                     GTSNLQQPSHLYNTQGIYNVQLAVQTQGGCRDTIMKPAVVKVINRPLIDINGDTVICI
                     NNSILHTGVFLRTDTSAVSWEWTFPNGVTYNVQNPPLQLYNVAGTFNITAYATNSSGC
                     KDTTRQTILVNPLPTVTMPGQLTINAGFPVTIPAVYSPNTLSWIWSPAIGLSCANCPT
                     PSAVPKINTFYQVYFTDANNCSNTGSIQINVLCGKGNLYIPNTFSPNGDGSNDFFYPR
                     GTGLERVKFLRIFNRWGEVVFEKKDFPVNNAASGWDGRFKGQKPKADVYIFQAEVFCE
                     NGELIQLNGNIALIL"
     gene            5324..6334
                     /locus_tag="PLU11_05715"
     CDS             5324..6334
                     /locus_tag="PLU11_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPN58645.1"
                     /translation="MYLRRIKHILFFVMASGWYTAPCAQDIHFSQFFEAPLWRNPSLA
                     GIFTGDLRVQAVYRDQWNSVTDAYRTGSVNAEYKMPVGKLNDFVTIGLQALYDRAGTI
                     GWTSTHLLPALNYHKSLSADKNRYLSLGFMGGLVQRRFDPSKMTTNSQYDNGGLGENL
                     LNTQYSYADGSVGMSYNSDIKDQPANNFYIGAAYHHFNRPRNSFYRNNTIELHPKWVF
                     SGGLRMGVADYAFITLQADHSRQGGFSETMGGVLYGLKLGEDPDNPAYIIHAGGFLRW
                     NDALVPVIKLDYAPFSVALSYDVNISKLKPSSYGRGGFEMSVSYIGFLKRNEGYLLCP
                     KF"
     gene            6404..6874
                     /locus_tag="PLU11_05720"
     CDS             6404..6874
                     /locus_tag="PLU11_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPN58646.1"
                     /translation="MAITIGQVAPDFTLFDSEKKPVTLSGLKGKNVLLLFFPQAFTSV
                     CTKELCGVRDNITRYQSVNASVFGLSVDSVFTLGKYKEEQQFNFPLLSDFNKEVSAAY
                     DTIYQDWILNMRGVSKRSAFVIDKEGIVRYAEVLESAGDVPDFDKINSVLDNLG"
     gene            7007..7348
                     /locus_tag="PLU11_05725"
     CDS             7007..7348
                     /locus_tag="PLU11_05725"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN58647.1"
                     /translation="MKRLLPILFLLILTTLQSEGQTSRTNPGDGDRIVKLYPNPATAY
                     ITFDLQANFKKGLSVQIYNGVLGKKMYESQISQDKFTVNLNDYTRGIYIYHLVDTTGK
                     IIESGKFQVSR"
     gene            complement(7373..8545)
                     /locus_tag="PLU11_05730"
     CDS             complement(7373..8545)
                     /locus_tag="PLU11_05730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPN58648.1"
                     /translation="MSSVYLKRKISPRVVNGHPWVFANEVEKIDGEVEGGDIVELFTH
                     DKKFVGKGYINPKSQILVRLLSRSKSDTINEEYFIERIRQCWEYRKKIGYTENCRLVF
                     GEADSLPQLIIDKFNDYFVIQTLALGIDVWKPALVKALNTVFQPKGIYERNDVPVREL
                     EGLPQLKGFLSAPFDTNIIINENGLRFHVDIENGQKTGYFLDQQDNRRAIRHIVKDAE
                     VLGAFTYTGTFEIHAAHYGAKSVLGLDISANAVTQANRNAELNGVQDRCRFEAANAFD
                     VLKQWGKDGRQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKLVRPGGFLVTSSC
                     TNLVHPELFLQTIEMAAKDARRRIRQVTFQSQASDHPIIWGMENTNYLKFLIVQVL"
     gene            8729..>9030
                     /gene="grpE"
                     /locus_tag="PLU11_05735"
     CDS             8729..>9030
                     /gene="grpE"
                     /locus_tag="PLU11_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPN58649.1"
                     /translation="MAEEIKEPQTTEPGQPAGMDINADETQSGTTHLNEPVAQENELE
                     QLKAELAEANDKYLRKVAEFENYKRRTAKERIELMQTAGRDVITDLLDVLDDCDRA"
     assembly_gap    9031..9130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9131..9453
                     /gene="grpE"
                     /locus_tag="PLU11_05740"
     CDS             <9131..9453
                     /gene="grpE"
                     /locus_tag="PLU11_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPN58650.1"
                     /translation="RDVITDLLDVLDDCDRAQKQLENNADVATQKAGVMLVFNKLRNS
                     LQSKGLKAMQTVHTEFDADLHEAITEIPAPAEELKGKVIDEVIKGYYLNDKIIRHAKV
                     VVGK"
     gene            9482..>10256
                     /locus_tag="PLU11_05745"
     CDS             9482..>10256
                     /locus_tag="PLU11_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPN58651.1"
                     /translation="MSKRDFYEILGVSKTATTDEIKKAYRKVAMQFHPDRNPGDKAAE
                     EKFKEAAEAYEVLSDADKKAQYDRYGHAGVSGNGRGGFSGSGGMNMDDIFSQFGDIFG
                     EDMFGNFFGGGGQRGGARGGQRNRGVRGSNLRVKLKLTYEEIAKGVTKNIKVKKHVPC
                     TTCNGSGAKDKGSVQSCGTCGGSGQVRRVQNTFLGQMQTVTTCPTCNGEGTTITAKCG
                     SCKGEGRVYGEETVSIDIPAGVQEGMQMNVSGKGNAGERG"
BASE COUNT         2688 a         2445 c         2356 g         2667 t
ORIGIN      
        1 acagctctac atacactatg gaactgaggg tatccggctg aaaatcggaa taaaaatagg
       61 ctttgaacca ggccgtttcg ccggcaacat aattatcacg gtcgcagtgc agccagattt
      121 tttcaaccgg cgctttctgg taccattgct ggaggagttc ttcgggtttt tggctgaatc
      181 cgttaataga gaaacaggta aaaacaagga agaggagcag ttttgttttc acaataaagt
      241 tataagtgct tgtgaaatta ccgaattaat caggaatggt acatccgctt gcgttaagac
      301 cgccttaaaa cctgtttaaa cgggtgaatt tacctgttgg ataagcagtt ttttagacct
      361 aatttgtgca agtaaaccgg aaatatgaaa agactgatca tggcctgcag cctgttgctg
      421 ttgttgacag gttgtaaaaa agcaattgaa aatgcccagg acgacctggt catacgggct
      481 atgacggatg gggagtgggt catcagcagc ttcacggtca atggaacccc aaccaccccg
      541 gattattccc tttacaaatt caagtattac agcaaccgga cggtggatgc cattaaaagt
      601 ggtaccgtag aaaaaaccgg gacctgggac gggaatacca gcaccatgat catttccgcc
      661 aattttactg ccgccactta tccgctgaat cagatcaacg gcagctggct gataacccgg
      721 aacagctgga cttatgtgga agccacacag actatagggt cggatgtgaa aacgctccgg
      781 ctggacaaac agtaatctta tatatccaat cccccttgtt aaactaaaat tccctgatta
      841 tgtctgattg agttgggaga aaagtcattt ttcttccgcc aaaaagaaat aatcacttca
      901 aaaactattc tgggaatagt ttttgtaagt aattaattcc caaaatatta aaccagctcc
      961 ctgttgaagc gacttataag ccatatccat tccggccgat tcctgctgct ttgcctgttc
     1021 ttttatagtt cagtggccaa tgcgcagccg gttgctaatt tcaccgcttc ccccctggcc
     1081 ggctgttcac ccctgattgt caattttcag gatctatcca ccggcactcc taccaactgg
     1141 ctctggaatt ttggaaatgg gaatacatcg acgttacaga accctattgc gtcctatttt
     1201 acaccgggga cttacactat tacccttacg gtaacgaatg ccggcggttc aaataccctg
     1261 gtccgtacta attatatcac tgtttacgag cccccgacgg ttgatttttc cgccagtatc
     1321 actacgggtt gtttcccctt acgggtacaa ctgaccgatc tttccaccgc aggagtaggg
     1381 aataccaatg tcagctggga ctgggatttc gggaacgggc agacttcaaa tcttcagaat
     1441 ccggtggtca cttataccac agcgggcatt tttaatgtga cgctccgggt taccaacgat
     1501 aaaggttgtg tgcgcactct cagccgcccg gcatttatta atgttacccc gggtgtccgc
     1561 tccttattca cgcataccgt acccactgta tgcagtgccc ctgccaccat tgactttacc
     1621 aataattcca cgggccctcc tgtcttaagc tacttgtggg atttcggaga tggtaataca
     1681 tccaccgcac aaaatccttc ccatacatac gttacgaatg gaacgtacac cgccaccctg
     1741 attactttca gttcggctgg ctgccaggat acccttcgca gtaacccgat cgttgttgga
     1801 ggaatcacca ccagtttttc gggtccggtt tccggctgtg taaatgaaca actgagtttt
     1861 accgttacct ccaacccggt gccgaatatt tccaactgga cattcgggga tggggctacc
     1921 ggtgtagggt tgaacgtaac gcatagctat gccgcggccg gtgtgtatcc gataaaactt
     1981 tacaacacgt atacaaattg tgtggattcc tttattcaga atatcacaat taacccgctg
     2041 cctgtagtcg atttcaccgc gccggtaacc gtgagctgcc agcctccact gacggtcaat
     2101 ttccaggacc tgactaccgg gggagcaact gcctggcaat gggatttcgg ggatgggaat
     2161 acgtccgccg tgcaaaatcc ttctcacact tataccagtt acggcaactt caccgtcacc
     2221 ctgattgcca ccaatggatt cggctgtaca gatactatag tcaagcccgg ttttatccgg
     2281 atccgccggg cattgatgtc ggtaaccggt ttgcctgaaa ggggatgtat cccttatacc
     2341 atcacgccgg tgccggtgat aaattcactg gatgcagtga cttcctggga atgggatttc
     2401 ggggacggag gtactgcttt tgtacagaat ccctcacaca cttatacggt tcaaggcact
     2461 tatacagtaa aactgattat caccaccagc agcggctgcc gggacacttt aaccctcaat
     2521 tccgcggtac aggttgggtc caagccaatg gccaatttca gcgccacccc caacccggta
     2581 tgcgcccgtc agcccgttca gtttaccgac ctgtctgttc cggctgatga atggctatgg
     2641 gattttggcg atggatcaac cagtattctg caaaacccga tacacaattt taatgatacc
     2701 ggttatttcg acattaccct gattgcctat aataacggat gcccggatac gatagttttc
     2761 acggattata tctatgtgct tcccccgatc gcacgggtta taatcggacc tgactgcaat
     2821 aaccgtttac gtttttcctt tattgatttt tccgtcgggg ctcttacctg ggaatgggat
     2881 ttcggggatg gcagtccgtt tgacaacaca caaaacccgg tacatactta tcccgcatta
     2941 ggcaactact ccgtccggtt ggttgtaacg aatggtggtt gtgcagatac aacagtcgtt
     3001 cctgtttacg ccatcgatga acgggcagat tttactgcca gccagacgac gctttgcaaa
     3061 atcggtacag tcaccttcaa taccctgaat gttaatccgg ccaatatcac ttcctatgca
     3121 tggaatttcg gggatggtaa ttcaacgacc accagcaccc ccttcgttac ccatacctat
     3181 accaacgtgg gcacttattc agtcagccta accatcaccg acctgaatgg ctgtaccagt
     3241 acggttataa aaacgaatta tatccgtatc aacggaccca cggctaattt catcgcaggc
     3301 aatgcatccg gttgtaccgg gctggttgcc acgtttacgg atcaatccac caccgatgga
     3361 cagaatgcca tcaccaactg gttctgggat tttggtgatg gcaatacaca gaattacacg
     3421 gccccaccct tccagcacac ttataattca gttggatcat tcaatgtaaa actgatagta
     3481 actgatgcgg ccggttgccg ggacagtctt acgctaatca gtaacgtaac cacttctgat
     3541 cctacaccgg actttgtggt cgacaccctt tcctgtccgg gtgctgtgct tacgtttacg
     3601 aataactcct tacctgccgg cgtcagcagt gcatgggatt ttggagatgg taatacttca
     3661 atcgtaacca gtcctactca cagctatgcg gctacgggtc tttatacggt gaaactggtc
     3721 atccgcgatg ccttaggttg ttcggattcg ctcatacgga ctaactatat ccgggtagcc
     3781 ctgcccgtag ctgacttcac tatgagtgac tctgtcactt cctgtacacc actggaaata
     3841 cagttcacga atacgtccac tttttacagt tcagtcagct ggaatttcgg caccggacaa
     3901 ggcatatcca ccctcaacaa cccgattcac ttcttttctt ctcctggcat atacccggtg
     3961 aaactgatag ttaccagtcc ggggggctgc cttgattcca ttataaagac agtccgtgta
     4021 tttgatacag ccggttcacg gattaactat actcctatcg gtggctgcaa accgctttct
     4081 attgccatgt ctgcgattac tgcaggcccg atggtttctt atttctggga cttcggagat
     4141 ggcaatacct taaccaccac cacccccagt gctaaccata tttatacctc attcggcagc
     4201 tttgtgccga gggtgatttt ggaagatcct tccggctgta ttattccttt gcagggggca
     4261 gacacgttgc tggtttccgg tgctactgcc aacttcggag tggatacctc tttcttctgt
     4321 gattatggta ccgtcaattt tattgactcc accacgttca atgatccgat tgtccagtat
     4381 agctgggatt ttggagatgg cgggacatca aacctgcaac aaccttcaca cttgtataat
     4441 acgcagggta tatataatgt gcaattagcc gtgcagaccc agggcggttg ccgggatacc
     4501 attatgaaac ccgctgtggt gaaagtgatc aaccgtccgc tgatcgatat taatggggac
     4561 acggtcattt gcatcaacaa ttcaatactg catactggtg ttttccttcg gacagatact
     4621 tcagccgtca gctgggaatg gactttcccg aacggggtga cttataatgt acagaacccg
     4681 ccattgcaac tttacaatgt agccggtaca ttcaacatta ccgcctatgc caccaacagc
     4741 agcggatgta aggataccac ccgccagacc attctggtga atcccttgcc cacggtgacg
     4801 atgcccggtc aactcaccat taatgccggc ttcccggtta ccatacctgc tgtctattca
     4861 cccaataccc tcagctggat ctggtcgccg gcaatcggcc tcagttgtgc caattgtcca
     4921 acaccatcag ctgtaccaaa gatcaatact ttctaccagg tctattttac cgatgccaat
     4981 aattgcagca ataccggcag tatacagatc aatgtattat gcgggaaggg caacctttat
     5041 attcccaata cgttttcacc caacggggat ggcagcaatg atttctttta cccacgtggc
     5101 acgggccttg agcgggtgaa attcctccgc attttcaacc gctggggtga agtggttttt
     5161 gagaaaaagg atttcccggt taataatgcg gcatccggtt gggatgggcg tttcaaagga
     5221 caaaagccaa aagcagatgt ctatattttc caggcagaag tcttttgtga aaatggagaa
     5281 ctgattcaat taaatggaaa tattgcgctg attctataat aaaatgtatc tacgaagaat
     5341 aaaacatatc ctgttttttg taatggccag cggctggtat acggctccct gtgcacagga
     5401 tatccatttt tcccaattct tcgaagcacc actctggcgt aacccttctt tggccggcat
     5461 ttttacaggc gatctccggg tacaggctgt gtacagggat cagtggaaca gtgtgaccga
     5521 tgcctaccgt accggatcag tgaacgcgga atacaaaatg cctgtgggca aactgaatga
     5581 tttcgtcacc atcggactgc aggcgttgta tgaccgggcg ggcactattg gctggacttc
     5641 cacccattta ctccctgctt tgaattatca taaatctctc agtgccgata aaaaccgcta
     5701 cctgtcgctt ggttttatgg gaggtctggt acaacgacgg ttcgatcctt ccaagatgac
     5761 cactaacagt cagtatgata acgggggcct gggcgaaaac ctgctcaata cccaatacag
     5821 ttacgcggac ggaagtgtgg gcatgagtta taattcagat attaaagatc agcctgctaa
     5881 taatttttat atcggggcgg cctatcatca ctttaaccgg ccgagaaatt ccttttaccg
     5941 gaataacaca atcgaacttc atcccaaatg ggtattttct ggtggcttaa gaatgggtgt
     6001 ggctgattat gcctttatca ccttgcaggc cgaccattcc cgccagggcg gtttcagtga
     6061 aaccatgggt ggggtgttgt atggattgaa actgggggag gatccggata accccgccta
     6121 tattattcat gccggtggtt tcctccgttg gaatgatgcc ctggtaccgg tgatcaaact
     6181 ggattatgcg cctttctccg tcgccctcag ctatgacgta aatatctcca aactaaaacc
     6241 ctccagttat ggccggggcg gatttgaaat gtccgtctcc tatatcggct ttctcaaacg
     6301 caacgaaggc tacctgcttt gcccgaaatt ttaaaaacag gattgtccgg gcgaaccttc
     6361 cagccataaa tttgtttcta tctcaactta aacaaattca tgtatggcta ttactatcgg
     6421 acaagttgct cctgacttta ctttatttga cagtgaaaag aaaccggtta ccctttcggg
     6481 tttgaaaggc aaaaatgtac tccttttatt cttcccccag gcctttacca gtgtgtgtac
     6541 caaagagctc tgcggcgttc gggataatat aacccgatac caatcggtga atgccagtgt
     6601 ttttggtctt tctgttgatt cggtatttac cctgggtaaa tacaaggaag aacagcagtt
     6661 taatttcccc ctgctgagtg actttaataa agaagtatcc gcggcgtatg acactattta
     6721 tcaggactgg atcctgaata tgcgcggtgt atctaaaaga tctgcctttg taatcgataa
     6781 agaggggatt gtccgttatg cagaagtact ggaaagtgca ggggatgttc cggattttga
     6841 caagatcaat tccgtgctgg acaacttagg ctaaaattaa gttaaaattt gcctgctacg
     6901 ccttgtaatt caatgattta caaggcgtag ttttgactag tagttttact attccttttt
     6961 caaaaaaagg ggttacattt aggataataa aagtaatttt gtttcgttga aaagactttt
     7021 acccatatta ttcttattga tactcaccac ccttcaatct gagggtcaga catcccggac
     7081 taatccgggg gatggagatc gtatagttaa gttatacccg aatccggcta ccgcttatat
     7141 tacgtttgat cttcaggcta atttcaaaaa aggactttct gtgcagattt ataacggagt
     7201 attaggcaag aaaatgtatg aatcacagat cagtcaggac aagttcactg ttaacctgaa
     7261 tgattatacc cgtggaattt atatttacca tctcgtggat accaccggca aaatcattga
     7321 gtctggcaaa ttccaggtat cccgttaatt ttccccaatt tattttaatg gattacagta
     7381 cctgaacgat caggaacttg aggtaattcg tgttttccat cccccagata atcgggtgat
     7441 cggacgcttg tgactggaaa gttacctgcc tgatacgacg gcgggcatcc tttgccgcca
     7501 tttcaatagt ctgtaaaaag agttcaggat gcaccaggtt tgtacaggaa gaagtaacga
     7561 ggaatccacc gggtctcacc agtttcatcc cacgaaggtt gatttcttta taaccggtaa
     7621 tggctttctg aatggtttca cggctcttgg taaaagccgg cggatccagc atcaccacat
     7681 cgtattgacg tccatccttt ccccattgtt tcagcacatc aaaggcattg gcagcttcaa
     7741 agcggcaacg gtcctgtacc ccgttcagct cggcattccg gttggcctgc gtcaccgcat
     7801 tggctgaaat atcgagtccc aataccgatt ttgccccata atgagctgca tggatttcaa
     7861 aagtgccggt ataagtaaag gctcccaaaa cctctgcatc tttcacgata tgccggatag
     7921 cccggcggtt atcctgctgg tccagaaaat aaccggtctt ttgtccgttt tctatatcca
     7981 catgaaaacg aagtccgttc tcattgataa taatattggt atcgaaagga gcggacagaa
     8041 atcctttcag ctgaggcaat ccttccagtt cccggacagg cacatcattg cgttcataaa
     8101 ttcctttggg ttgaaagacc gtattcaggg ctttcaccag ggcgggcttc cagacatcaa
     8161 tcccgagtgc cagggtctgg atcacaaaat aatcgttgaa cttatcgatg atcagctggg
     8221 gcaatgaatc ggcttccccg aaaacaaggc ggcagttttc tgtatacccg atttttttcc
     8281 ggtattccca gcattgacgg atccgttcaa taaagtattc ctcattaatc gtatcggatt
     8341 tactccggga cagtaatctt accaggattt gtgatttggg attgatatat ccttttccga
     8401 caaacttctt atcgtgcgtg aataattcca cgatatcccc tccttccact tcaccatcaa
     8461 tcttctccac ttcgttcgca aaaacccagg gatgaccgtt gaccacccgg ggagatatct
     8521 ttcgcttgag gtatacactg ctcataaggc ggcgaagata atggaaagcc ggttatggcg
     8581 gcagtcagtc tgaaggcaac tctgccagca caagatccga actaactgat tttcagtaac
     8641 tgggacctgg gtatacaggt cattttgtcc caaatatcgg gttggcaata taattgacaa
     8701 tcgtaccttt gcacacaatt tgtaaaaaat ggcagaagag attaaagaac cgcaaactac
     8761 tgaaccggga caaccggccg gcatggacat caatgctgac gaaacccagt caggaaccac
     8821 ccacctgaat gaaccggtgg cccaggaaaa cgagttggag cagctcaaag cggaactggc
     8881 agaagccaat gataaatacc tccgcaaggt ggctgagttt gaaaactaca aacgccgtac
     8941 ggccaaggaa cggatcgaac ttatgcagac agccggaagg gatgtgatca ccgacctgct
     9001 ggatgtactg gatgattgtg accgggccca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn gaagggatgt gatcaccgac ctgctggatg tactggatga ttgtgaccgg
     9181 gcccaaaaac aactggaaaa caatgctgat gtagctaccc agaaagcggg cgttatgctg
     9241 gtgttcaata aactccggaa ttcactgcag tcgaaaggat tgaaagccat gcaaacagta
     9301 catactgaat ttgatgccga cctgcatgaa gccattacgg agatcccggc acctgcggaa
     9361 gaattaaaag gtaaagtgat cgacgaagtg ataaaaggat attaccttaa tgacaaaatc
     9421 atccgccatg cgaaagtggt ggtgggaaaa taaatgaact attaacggat caatcaagca
     9481 aatgtccaaa agagattttt acgaaatact aggcgtcagc aaaacggcca ccacggatga
     9541 aatcaagaaa gcgtaccgga aggtggccat gcagtttcac ccggaccgta atcccggtga
     9601 taaagcggcg gaggaaaaat tcaaggaagc cgctgaagcc tatgaggtat taagcgatgc
     9661 agataaaaaa gcccagtatg accgttacgg acatgccggc gtgagtggca acggtcgtgg
     9721 tggattcagt ggcagcggcg gaatgaacat ggatgatatc ttcagccagt ttggggatat
     9781 tttcggagaa gacatgtttg gtaatttctt tgggggtggc ggacaacggg gtggagccag
     9841 gggcggacaa cgcaaccggg gtgtaagagg aagtaacctc cgggttaaac tgaaactcac
     9901 ctatgaagaa attgcaaagg gcgtaaccaa gaacatcaaa gtaaaaaaac atgtaccctg
     9961 taccacctgc aatggcagtg gggcaaaaga taaaggcagt gtgcagagtt gtggcacttg
    10021 cggcggaagt ggccaggttc gacgggtaca aaatactttt ctgggtcaga tgcagaccgt
    10081 caccacttgt cctacctgta acggcgaagg aaccacgatc accgctaaat gcggatcctg
    10141 taaaggagaa ggacgggtgt atggggaaga gacggtcagt attgatatac cggcaggtgt
    10201 gcaggaaggc atgcagatga atgtatccgg taaaggaaat gcgggagagc gtggcg
//
LOCUS       DAOPMW010000078        10254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7061_length_10254_cov_6.963428, whole genome shotgun sequence.
ACCESSION   DAOPMW010000078 DAOPMW010000000
VERSION     DAOPMW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7061_length_10254_cov_6.963428"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLU11_05750"
     CDS             <1..1167
                     /locus_tag="PLU11_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58652.1"
                     /translation="PTASHTYLTIGTYNVMLVAIDPATCNVRDSSFMKIRVGNLLATP
                     AFIPVKLPPCGSFNYRFDNTTIAPPTKPFTNQSFVWDFGDGSPTVVAGAAPVFHTYSG
                     PGTYNVKLLLQDTVYCNAPDSLVIPLSVADNVKAQFTTPPSGCIPYTPVFDNTSLAGQ
                     TFLWNFGDGNTSTAANPTYTYNTPGTYIITLVAYNPNTCNLTDTARFTIQVYGAPTAD
                     FAFSPLTPAPNMPVTFNNLSSLDAVSFKWEFGDGDTLRTTSRVNIDHQYNSTGTFNAC
                     LTAYNLIGCPAQICKPVSATVIPVVDVPNAFTPQSGDQNSVVMVRGFGIAKMQFTIWN
                     RWGQKVFETNDRNRGWDGKVKGVIQPMDVYAYTLSVEFFDGVKTTKKGDITLIR"
     gene            1187..2212
                     /locus_tag="PLU11_05755"
     CDS             1187..2212
                     /locus_tag="PLU11_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPN58653.1"
                     /translation="MKRYILILCCCGWFIAPAQDLHFSQFMNSPLLTNPANTGFMPDG
                     DYRVGINYRNQWASVTSFPYKTMSAFGDVQVLQNSDNNGWLGVGGMILRDVAGTSVLT
                     STKVYGSVAYHQMINAGSLVSLGFNVGWANKNINTSNLSFPSQWNGQFFDAHQTTVAP
                     KLDYNNINYLDMQVGMNYAYFPNDRAYLNAGFSAMHINQPKESFFNDQPGIDNRIPVR
                     YNGFLNGSFKINDMVILNPNAYVSVQAKSYEIVGGLNAQYNLSGDGEKTLTAGLYYRY
                     KDAVIPMIGLGVKDIVFTFTYDATMSGLKNFNNTRGAFEFSLVKQGAMGSYKGNKRES
                     MCPSFKY"
     gene            2286..2780
                     /locus_tag="PLU11_05760"
     CDS             2286..2780
                     /locus_tag="PLU11_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58654.1"
                     /translation="MNNISEGTNSVSQELKGLRILTTALSVGVVIFTLVSVGINQFNG
                     PFLQEEIKKVSSFLLPLMTGLALLALLMARYLYQQRIRDIREGNDNLKGKMDKYRAAV
                     TLYLAICEGMALFSVVLFLLSGDFLLLVITGAMLLLMLGRLFAIRGVANELNLSWQEQ
                     EELK"
     gene            2797..3378
                     /locus_tag="PLU11_05765"
     CDS             2797..3378
                     /locus_tag="PLU11_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58655.1"
                     /translation="MIKKLLTVLFISLFFTAAMAQDEEEERGFKANRVFIGSGVNLGF
                     SNGFIIGLNPEFGYSLNQVIDVGIATNLTYVTQRYQSVNITARYLALGGGPFIRIWPI
                     QQLFIGGQFEYNKISYSEKTDAGIINKAKATAPSLLVGLGYGTRNIGRGQFYTSIMID
                     AMKDFNSPYRDQFGRILPVFRTGFSFYLGRKKE"
     gene            complement(3386..3925)
                     /locus_tag="PLU11_05770"
     CDS             complement(3386..3925)
                     /locus_tag="PLU11_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPN58656.1"
                     /translation="MAIQSLAVFCGSKNGTNPVFSQHAAELGKLMAENNIRLIYGGGS
                     AGLMGIIADSVMLYGGAVTGIIPKLLLEWEVQHRGITELIISDDMHERKRKIYSISDA
                     ALILPGGFGTLDELFEIVTWNQLTIHDKQIYILNSGGFYDHLIRHIEVMKAEGFLYED
                     AIKRIQVIDSPAALLPFII"
     gene            4028..5560
                     /locus_tag="PLU11_05775"
     CDS             4028..5560
                     /locus_tag="PLU11_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPN58657.1"
                     /translation="MSPVVLFSFVIGYFALLLGVAWYTSRNSDNESFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGGVGTGNFYYYQIVLGYLLGYMVIAFVLIPLYYRMNLTS
                     IYTYLEKRFGINAHKAGAFFFILSRTVGATARLYLVISVLQIFIFNKLGIPFEATALV
                     ILVLILLYTFEGGVKTIIYTDTLQTTGMLVGLVVCIVVIVKALGTDFGGALNLMADKG
                     YTKIFNWDVNSGSFAIKHIVGGMFIAIAMTGLDQEMMQKNISVKTLKDSQKNIMTFTA
                     VLMFVNLLFLVLGGVLYLYAAKNGINVPPDDLFPTIALSDTFSGVIGVIFIIAIISAL
                     FPSVDGAITSLTSCYCIDILGINKQQGTEKGKKRTRLKVHFTFAAIFFLMVLLFKWIN
                     DKLIIDFILKFAGITYGPLLGLFSFGILTRRKLNDKLIWVACIAAPLLALGVDMLSNP
                     AWYEAKLHISLPLLTEWSASTFNGYKIGNELILINGIFTFCGLWLISRKGSPSEGSLL
                     AV"
     gene            5587..6225
                     /locus_tag="PLU11_05780"
     CDS             5587..6225
                     /locus_tag="PLU11_05780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPN58658.1"
                     /translation="MDLINPLAEAYADTYTSPEDSLLQEVAEYTRTRHSESIMLSGKV
                     QGKFLEMISFMLRPSAILEIGTFTGYSALCLAKGLTPEGRLHTIELREADSMLARGWF
                     DRSPRSGQIISHTGNALDVIPGLSETWDLVFIDADKPAYIDYFNLVFPRLRKNGFILA
                     DNVLFHGQVLEEPVKGKSAKGIQAFNEFIRQRTDIEKSILTVRDGLYLIRKL"
     gene            6265..7269
                     /locus_tag="PLU11_05785"
     CDS             6265..7269
                     /locus_tag="PLU11_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPN58659.1"
                     /translation="MTIVLIAIKSGAQQNETVLNYVSTYKELAMAEMKRTGVPAAIKL
                     AQGIHETMAGTSKLVLKSNNHFGIKCKTGWTGESVSHDDDARGECFRKYPSAEDSYRD
                     HSNFLKNSQRYAGLFQLDPTDYAGWANGLKKAGYATNPRYPLVLIKLIEDYNLQNYTL
                     LALGKTPVIDTEAEKTYETETGKVKVPEREPETTEAPVVAVNYPKGEFRINETRVVFV
                     KKGVSFLSLAQQYNIPLARIFDFNELPVTEALARDQLIFLQRKRKTGLDEFHKVMPDE
                     SVYDIAQKQGIRLESLRELNFLGPDDRPAPGEKLSLRKKAGAMPKLVIRENYSLYSTN
                     "
     gene            7316..7849
                     /gene="hpt"
                     /locus_tag="PLU11_05790"
     CDS             7316..7849
                     /gene="hpt"
                     /locus_tag="PLU11_05790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPN58660.1"
                     /translation="MSLIKVHDKTFDIYLSAETIQQRVKELADQINTDYAGKRPLFIA
                     ILNGSFMFAADLFKNLTIEAEICFIKLASYKGMKSSGNVVTSIGLEDDLFGKEVIIVE
                     DIVDTGKTLHNFLPKLGHQQPKSLRIATLLHKSDATVFPLHPDYVGFVIPDKFVVGYG
                     LDYDGLGRNLKEIYQVV"
     gene            7932..9671
                     /locus_tag="PLU11_05795"
     CDS             7932..9671
                     /locus_tag="PLU11_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPN58661.1"
                     /translation="MFLGHSLAAQQFYVRGEVKDEAGNVLQNVVIKQFKTGYVFRSGA
                     SGTFGILAHSQLDTFSFMLDGYHPEKMLVNADNYIRVKLKPAPASASNRFKDRLSSFT
                     KDLGREEQKKWFAGDESYASLLENRFLIARQFPSTGMSLNVDRASYSNIRRFITMNSI
                     VPPDAVRIEEMLNYFNLDYYPPAGDDLFYIKPTLTACPWNPGNQLYYINLSSRKLNLD
                     TLPPSNLVFLIDVSGSMDMPNRLPLLQSAFRLLVNNLRNKDSVSIVVYGGVTGVMLET
                     TSGAEKEKIIKAIDELTPGGSTPGEAGIRLAYTQARRHFIQGGNNRVILATDGDFNVG
                     LKTESELDEMISAQRESGVYLTCLGVGMGNYKDSKIQLLAEKGNGNFAYLDNFKEAEK
                     VLLKEFTQTLYSVADDVYMNVEFNPEYVKEYRLIGFDNKVGALRDASSVVEGGEIGSG
                     FSMIAMFEIEPTELSRGAIRDSIYSGQFANIRLQYRHPGDTTHRYFDRKSNFDYRAYE
                     KLEKCYHFSAAVAQFGSMLRNSPFVKNRTWNDMVEMAQASAGDADLLQKEFVNLTIMA
                     RNLYMKGKKRKSQ"
     gene            complement(9822..>10254)
                     /locus_tag="PLU11_05800"
     CDS             complement(9822..>10254)
                     /locus_tag="PLU11_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPN58662.1"
                     /translation="VSGKGNAGERGGMPGDLIILIEEEPHKELYREGLNVAYELHLSF
                     TDAVFGTQVEVPTIDGRAKIKIPAGTQSGKIFRLKGKGFPAVNSYEKGDQLIQVSVWT
                     PQQLSAEEKAILEKLGHSPNFKPHPEKSDKNFFDKIKEMFS"
BASE COUNT         2674 a         2351 c         2416 g         2813 t
ORIGIN      
        1 cctactgctt cacataccta cctgacaatc ggtacctata atgtaatgct ggtggccatc
       61 gacccggcta cctgtaatgt gcgggacagt tcgtttatga agatcagggt gggaaacctg
      121 ctggcgaccc cggcctttat tccggttaaa ctgcccccct gtggatcctt caattaccgg
      181 tttgataata cgaccattgc cccgccaacc aaacctttca ccaatcagag ttttgtctgg
      241 gatttcgggg atggttcacc caccgtagtg gcaggtgcgg ctcctgtttt tcatacctac
      301 tctggtcctg gtacttacaa tgtaaaattg ttgctgcagg ataccgtgta ttgcaatgcc
      361 ccggattcat tagtcattcc attgagtgta gcggataatg taaaagcaca attcaccaca
      421 ccgccttcgg gatgtattcc ttatacaccc gtatttgata atacttcact ggcgggacaa
      481 accttccttt ggaattttgg agatggcaat acgtccacgg cagcaaatcc aacctatacg
      541 tataacacac cgggaacata tattattaca ctggtggctt ataacccgaa tacctgtaac
      601 ctgaccgata cggcaaggtt tactatacaa gtttatggag cccccacggc agattttgct
      661 ttttctccgt taacacctgc tcccaatatg ccggttacat ttaacaatct ctcttcgctg
      721 gatgccgtca gttttaaatg ggagtttgga gatggggata cattacggac tacttcaaga
      781 gtcaatattg atcatcaata taattcgacc ggtacattta atgcctgtct caccgcatac
      841 aaccttatcg gttgtccggc gcagatctgc aagccggtca gtgctacggt gatacctgta
      901 gtggatgtgc ccaatgcctt cacgccgcaa agcggggatc agaacagcgt ggtgatggtt
      961 cgcggttttg gtattgccaa gatgcagttt actatctgga accgctgggg tcaaaaggta
     1021 tttgaaacga atgaccgcaa caggggatgg gatggcaaag tcaaaggcgt gatacaaccg
     1081 atggatgtat atgcctatac tttatccgtg gaattctttg acggggtcaa gacaacgaag
     1141 aaaggtgata ttaccttaat ccgataattg ttgaatcatg aagggaatga agcgatacat
     1201 actgattctt tgctgttgcg gatggttcat cgcccctgca caggacctgc atttttcaca
     1261 attcatgaat agccccctgc tgaccaatcc ggccaacacc gggtttatgc ccgacggcga
     1321 ttaccgggtc ggaatcaact accgtaacca atgggcttcc gtaacttctt ttccctacaa
     1381 aaccatgagt gccttcggcg atgtgcaggt attgcagaac agcgataaca acggatggct
     1441 gggtgtggga ggaatgatct tacgggatgt ggccggtacc agtgtgctta cctccactaa
     1501 agtatatggc tcggtagcct atcaccagat gatcaatgcc ggaagcctgg tgagtctcgg
     1561 atttaatgta ggatgggcca ataagaatat caatacgtcc aatctcagtt ttcccagtca
     1621 gtggaatggc cagttctttg atgcccacca gaccacggtc gcaccaaaac tggattacaa
     1681 caatatcaac tacctcgata tgcaggtggg aatgaattat gcctattttc ccaatgaccg
     1741 ggcttacctg aatgccggtt tttccgccat gcatatcaat cagccgaaag aatccttttt
     1801 taatgaccag cccgggatcg ataaccggat tccggtccgg tacaatggat tcctgaacgg
     1861 tagtttcaaa atcaatgata tggtgattct gaatcccaat gcctacgtat cagtacaggc
     1921 taaatcatac gaaattgtag gcggactcaa tgcacaatac aatctctccg gtgatggcga
     1981 gaaaacactc accgccggtc tctattaccg ctacaaagac gcggtgatcc cgatgatcgg
     2041 actcggtgta aaggatattg tgttcacatt tacctatgat gccacgatgt caggattgaa
     2101 gaacttcaat aatacccgtg gcgcttttga gttttccctg gtcaaacagg gggcgatggg
     2161 aagttacaag gggaataaac gggaatccat gtgcccctct ttcaaatact gatcttcctg
     2221 aaatactaag tttaccattc atccgttaaa ttgcataggt ttcgggttac catttttaat
     2281 aaagtatgaa taacatatct gaaggcacaa actctgtcag tcaggaatta aagggattac
     2341 gtattctgac cacggccctt tctgttgggg tcgttatttt tacccttgtg tccgtgggca
     2401 taaatcagtt caacggaccc ttcctgcagg aagaaatcaa aaaagtaagt tctttcctgt
     2461 tgccactgat gaccggactg gctttgctcg cactgctgat ggctcgttac ctgtatcagc
     2521 aacggatccg tgatatccgt gaagggaatg ataacctgaa agggaaaatg gataaataca
     2581 gagctgcggt aacgttgtac ctggccattt gcgaaggaat ggctctgttc agtgtggttt
     2641 tatttctgct cagtggcgat tttcttctcc tggtcattac aggagccatg ctgctgctga
     2701 tgctgggacg tttatttgcc atcaggggag tggccaatga actgaatctc agctggcagg
     2761 aacaggaaga actcaaataa agtatcaaaa ttaactatga taaagaaact gcttactgta
     2821 ctcttcatca gtttattttt taccgcagcc atggcgcagg atgaggaaga agaaagaggc
     2881 ttcaaagcca accgcgtttt tattggttcc ggtgttaacc tcggattttc caacggattt
     2941 ataatcggat taaatcctga attcgggtat agtctcaatc aggtaatcga tgtgggaatc
     3001 gccaccaacc tgacttatgt gacgcagcgc tatcaatccg tgaatatcac cgcccgttac
     3061 ctggccctcg gcggaggtcc cttcatccgg atctggccca tccagcagct ttttatcggc
     3121 ggccagtttg aatacaacaa gatttcttat agtgaaaaaa cggatgccgg tatcattaat
     3181 aaagcgaaag ccacggcgcc cagtttgttg gtaggactgg gctatggtac ccgcaatatt
     3241 ggccgcggtc agttctatac cagtatcatg atcgatgcca tgaaggattt taattcgccg
     3301 taccgcgatc agtttggccg gatactacct gtattccgca ccggttttag cttttacctg
     3361 ggacgtaaaa aagaataata ccggtttata ttataaaggg caataatgca gcggggctgt
     3421 caattacctg gatccgttta atcgcatcct catacaggaa gccttccgct ttcatgactt
     3481 caatatgccg gatcaggtgg tcgtagaatc cgccagagtt caggatatag atctgcttgt
     3541 catggatagt gagctggttc caggttacga tttcaaataa ttcatcgagg gtgccaaaac
     3601 ctcccggcag gatcagggcc gcatcagaga tactgtagat ctttcgtttc cgctcgtgca
     3661 tgtcatcact gatgatgagt tctgtaatac cgcggtgctg tacttcccat tccagtaata
     3721 atttcgggat gatgccggtc acggcgccac catacagcat tacactgtcc gcgatgatac
     3781 ccatcagtcc ggcactgccc ccgccatata tcagccggat attgttttcc gccatcagtt
     3841 ttcccagttc agctgcatgc tgactgaata caggattggt tccgttcttc gaaccgcaga
     3901 aaaccgcaag tgattgaatc gccatataaa taatattgac gcaaagggat aacattttta
     3961 attatcttca aaattcatta ttgcctattt tgcaacgcta ataattgctt aacagaaacc
     4021 gccaactatg tctccagtcg ttctcttttc ctttgtgatc gggtattttg cacttttact
     4081 cggggtagcc tggtatacct cgcgtaattc ggataatgaa tccttcttta tcggcaaccg
     4141 caacagtaac tggatgctgg tggcttttgg tatgatcggt acatcactgt ccggggttac
     4201 gtttgtgagt gtacctggtg gggttggaac cggcaatttt tattattacc agatcgtact
     4261 gggctacctg ctggggtata tggtgatcgc ctttgtgctg attccgttgt attaccggat
     4321 gaacctgacc agtatctaca cttacctcga aaaacgcttt ggcatcaatg cccataaggc
     4381 cggtgctttc ttctttatac tttcccgtac ggtaggtgcc accgcccggc tctacctggt
     4441 gatcagtgta ctgcagattt ttatatttaa taaactgggt attccatttg aagccaccgc
     4501 gctggtaata ctggtgctca tcctgctcta tacttttgaa ggcggggtga agaccattat
     4561 ttataccgat acgctccaga ccaccggtat gctggtgggc ctggtggttt gtatcgtggt
     4621 gattgtaaaa gccctgggca ctgattttgg cggcgccctg aacctgatgg ccgacaaagg
     4681 ctatacaaaa atctttaact gggatgtcaa ctccggctcc tttgcgatca aacatattgt
     4741 gggagggatg tttatcgcca ttgccatgac cggactcgac caggaaatga tgcagaaaaa
     4801 tatcagtgtg aagaccctga aagattcaca gaaaaatatc atgaccttca ccgcggtact
     4861 gatgtttgtt aacctgctct tcctcgtgct gggtggcgta ctttatctct acgcagcgaa
     4921 gaatggcatc aatgtaccac ctgatgattt attcccaact attgctttga gtgatacctt
     4981 cagcggggtt ataggggtga tctttatcat cgccatcatc tccgccttat tcccgagtgt
     5041 ggacggagcc atcacttccc tgacctcctg ttattgtatc gatatcctgg gtattaataa
     5101 acaacaaggc actgaaaagg gaaagaagcg tacaaggttg aaagtgcatt tcacgtttgc
     5161 cgctatcttt tttcttatgg tccttttatt caagtggatc aacgataaac tgatcatcga
     5221 ctttatcctc aagtttgccg gtatcacata tgggcctttg ctgggactct tctcttttgg
     5281 tattcttacc cggcgaaagc tcaacgacaa actgatctgg gtggcttgta tagccgcacc
     5341 gttattggca cttggtgtag atatgctcag caacccggcc tggtatgaag ccaagctgca
     5401 tatcagttta cccctgctga cggaatggtc ggccagtacc tttaacgggt ataagatcgg
     5461 gaatgagctg atccttataa atggcatctt tactttctgt ggtctctggc tgatttcaag
     5521 aaaagggtca ccttcagaag gtagtctgct ggccgtataa gtttatcttt gcaaaaaacc
     5581 ggcaggatgg atctgatcaa cccgttggcg gaagcctatg cggacaccta tacttcacct
     5641 gaagacagcc tgcttcagga agtggctgaa tatacccgca cccgtcattc cgaatccatc
     5701 atgctcagtg ggaaggtcca ggggaaattc cttgaaatga tcagtttcat gcttcggccc
     5761 agtgctatcc tggagatcgg cacatttacc ggctatagtg ctctctgtct ggccaaagga
     5821 ctgaccccgg aagggcgcct gcataccatt gaactccggg aggccgattc catgctggcc
     5881 agaggctggt ttgaccgttc gccccggtcc ggccagataa tatcacacac gggtaatgcg
     5941 ttagatgtca ttccaggtct gtctgagacc tgggacctgg tctttattga tgccgataaa
     6001 ccagcctata tcgattattt taaccttgtt tttccccggt taaggaaaaa cggctttatt
     6061 ttagcagata atgtgttgtt tcatggacag gtgctggagg aaccagtgaa ggggaaaagt
     6121 gccaaaggca ttcaggcctt taatgaattt atccggcaac gtacggatat tgaaaaatca
     6181 atactcacgg tccgggacgg gttatacctg atccggaaat tataatcagt tggaatgatg
     6241 ttacggaaat cagcgatatt ctatttgacc atagttttaa tcgcaataaa gagtggtgcg
     6301 caacagaatg agacagttct caattatgtc agcacgtata aggaactggc gatggccgaa
     6361 atgaaaagaa ccggggtgcc ggcggctatt aaactggcgc agggtattca tgaaaccatg
     6421 gcgggtacca gcaaactggt actgaaatca aataatcatt tcggtataaa atgcaagacc
     6481 ggctggaccg gtgaatcggt ttcccatgat gatgatgctc ggggtgagtg tttccgcaag
     6541 tacccttccg ctgaagactc ataccgggac cattccaatt tcttaaaaaa cagtcagcgc
     6601 tatgccggtt tgtttcagct cgatcccact gattatgccg gctgggccaa tggattaaag
     6661 aaggcaggct atgccaccaa tccacgttat ccactggtgc tgatcaaact gattgaagat
     6721 tataatctgc agaactatac cctgctggca ctggggaaaa cgccggtcat tgatacggaa
     6781 gcggagaaaa cctatgaaac agaaacaggg aaggtgaagg ttccggaaag ggaaccggaa
     6841 acaacagaag ctccggtggt ggcagtgaat tacccgaaag gtgaattcag gatcaatgaa
     6901 accagggtcg tatttgtaaa gaagggggtt tcctttctct ccctggcaca gcaatacaat
     6961 attccgctgg cccgtatatt cgatttcaat gaactgccgg tcaccgaggc attagccagg
     7021 gatcagctta tttttttaca acggaaaaga aagaccggtc tggatgaatt tcataaggtg
     7081 atgccggatg aatcagtata tgatatcgcc cagaaacaag ggatacggct ggaaagcctg
     7141 cgcgaattaa atttcctcgg tccggatgat cgtcccgctc ccggcgaaaa actcagtctc
     7201 cgtaaaaaag ccggcgctat gcccaaactc gtcatccgcg aaaactacag tctctattcc
     7261 accaactaaa ccactaaacg tctaaacgac taaacaccta aacctttcta acgctatgtc
     7321 ccttatcaaa gtccacgata aaacgttcga tatctatctt tccgcagaaa ctattcagca
     7381 acgggtcaaa gaactggcgg accagattaa taccgattat gccgggaaac gtccgctgtt
     7441 cattgccatc ctcaatggtt ctttcatgtt tgccgctgat ctctttaaaa acctgaccat
     7501 tgaagccgag atctgtttta tcaaactggc atcctataaa ggaatgaagt cttccgggaa
     7561 tgtggtaacc tccatcggac tcgaagatga tttgtttgga aaggaagtga tcattgtaga
     7621 ggacatcgtg gatacgggga agaccctgca taatttttta cccaagctgg gacaccagca
     7681 acccaaatcc ttacggatcg ccactttgtt acataagtca gatgcgaccg tttttccgct
     7741 gcatcccgat tatgtaggct ttgtgatccc tgataaattt gtagtaggat atgggctcga
     7801 ttatgatgga ttgggacgta acctgaaaga gatttaccag gtggtatgat ttatcggtca
     7861 tattgccgtc tatttagtaa tttataagat atactaaccg gttgaagacc acttgccata
     7921 tattaatcct tttgttcctg ggccactcac tcgctgctca gcagttttat gtgcgggggg
     7981 aagtgaagga tgaagcgggt aatgtgctgc aaaatgtagt tattaaacag ttcaaaacag
     8041 gttatgtatt tcgtagtgga gcttcaggta catttggtat cctggcccat agccagctgg
     8101 atacattcag ttttatgctg gatggctatc atcccgagaa aatgctggtg aatgcggata
     8161 attatattcg ggtcaaatta aagccggccc cggcttctgc cagtaaccgg ttcaaggatc
     8221 gtctttcttc ctttaccaag gatcttggaa gagaggaaca aaaaaaatgg tttgccggtg
     8281 atgagagtta tgccagctta ctggaaaacc gtttcctgat tgcccggcag tttccgagta
     8341 ccggtatgtc actcaatgta gaccgggctt cttacagcaa tatccgccgc tttattacca
     8401 tgaattccat cgtgcctcct gatgccgtac gtattgagga gatgctcaat tatttcaacc
     8461 tcgattatta tccacctgcc ggggatgatc ttttttatat taaacccacc ctgactgcct
     8521 gtccctggaa tcccggcaat caattatatt atatcaacct ttcttcccgg aaactgaacc
     8581 tcgataccct gcctcccagc aatctcgttt tcctgatcga tgtgtccggc tccatggata
     8641 tgcccaaccg cttaccctta cttcaatccg ccttccgcct gctggtaaat aacctgcgca
     8701 acaaggactc ggtgtcaatt gtagtctatg gcggggtgac cggggtaatg ctggaaacca
     8761 ccagcggggc agaaaaagaa aaaattatta aagccattga tgaactgacg cctggtggtt
     8821 cgactcccgg agaagcaggt atacggcttg cctatacaca ggcaagacgg cattttatcc
     8881 agggaggcaa taaccgggtg atcctggcca ccgatgggga ttttaatgtg gggttgaaaa
     8941 cagaatctga gctcgacgaa atgatcagtg cccagcggga gtcgggagta tacctgacct
     9001 gcctgggtgt gggtatgggt aattataaag actcgaaaat ccagttgctt gccgaaaaag
     9061 ggaatggcaa cttcgcgtat ctcgataatt tcaaagaagc ggaaaaagta ttactgaagg
     9121 agttcaccca gaccctttat tccgtagccg atgatgtata tatgaatgtg gaattcaatc
     9181 cggagtatgt gaaggaatac cggctgatcg ggtttgataa taaagtggga gccttgcggg
     9241 atgccagctc cgtggtggaa ggaggggaga tcggttccgg gttttccatg atcgcgatgt
     9301 ttgaaattga acccaccgaa ctcagccgtg gagctatcag agacagtatc tattccggac
     9361 agtttgcgaa tatccgcctg caataccgtc atccgggcga taccacccat cgttactttg
     9421 accggaaaag caatttcgat tacagggctt atgaaaagct ggaaaaatgt tatcacttct
     9481 ccgctgctgt agcccagttc ggttcaatgc tacgcaactc tccctttgta aaaaacagga
     9541 cctggaatga tatggttgaa atggcacagg cttctgccgg tgatgctgat ctgttacaga
     9601 aagaatttgt gaaccttacg ataatggcga ggaacttgta tatgaaggga aagaagagga
     9661 agagtcagta ggcagtagac agtaggcagt tggcagtcaa cagccaatac aaaataacca
     9721 aaccttctta ggaggtttgg ttattttagt ttgatactta tttgaatttt gttatttgat
     9781 tattctttgg cgtcttggca tctttggttc ttggaattta gttacgagaa catttccttg
     9841 atcttatcaa aaaaattctt gtcactcttc tcgggatgtg gtttgaaatt cggggagtgg
     9901 cctaattttt ccaatatagc tttctcttct gcactcagtt gttgtggggt ccaaacactc
     9961 acctggatca gttggtctcc tttttcgtag gaattgacgg ccgggaatcc ctttcctttc
    10021 aggcggaaaa ttttaccact ctgggtgccg gcaggaattt taatcttggc gcggccatca
    10081 atagtgggca cttccacctg cgtaccaaaa accgcatcag tgaaagagag atgcaattca
    10141 taagccacat tcagtccttc gcggtataat tccttatgcg gttcttcttc aatcaggatt
    10201 atcaggtcgc ccggcatacc gccacgctct cccgcatttc ctttaccgga taca
//
LOCUS       DAOPMW010000079        10229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7086_length_10229_cov_9.999017, whole genome shotgun sequence.
ACCESSION   DAOPMW010000079 DAOPMW010000000
VERSION     DAOPMW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_10229_cov_9.999017"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLU11_05805"
     CDS             complement(<1..789)
                     /locus_tag="PLU11_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN58663.1"
                     /translation="MNFYLSGLYFLPLLIGGILLIAPAAISRIIVILSSILLSALALY
                     VFIHVNEPLYFTVPHYVNEIVAGADILMLLYFGWVAIQKKSWLVGVMSIVQLAGLLYL
                     LKNLPAGESMPFMVDKLSTFMFLLINIISGIIAIFTLRYIEEENCSAFRKKFFLALLF
                     FFIGAMNLVVSADNLEYFFLFFELTTLASFLFIGFRKDEVSVKNALTALWMNQVGGIA
                     ILAAIFFIAYNGYGEITFSNLLAHANAEGILLPLALLSIAALIKG"
     gene            1100..2239
                     /locus_tag="PLU11_05810"
     CDS             1100..2239
                     /locus_tag="PLU11_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58664.1"
                     /translation="MAKFTLLLFVLSACLFFSQLTAQDLHPLYLSASEKYKVKDYKGA
                     IADCNKLLKIKPGDFDATYLIAYAKYELKDFPAALEDANKLLKKENPTWTIADLYYFR
                     GLIYLELNQHQNACNDFTRKIDQRPLLSPFAYFKRGLAKINLNDYPGAIQDFTKAGVQ
                     CPTCDEFFKFNALAKVKTNDLKGALADYNRLIELKPDESEFYKERGEVKSGLEDNEGA
                     MADFNKAISINPKNPVYYYDRASVLTAKEDYYGAIKDLQKAAELNPRIEEIYIAISDN
                     YIGLNDFEKALRYINMAISVNTKNIFTLNLRGLLKSKINDYAGALADYNNNILLFPNE
                     WRTYVLRGKLKFEHLADKAGACSDFRKAKELGSDSAAQLIQENCN"
     gene            complement(2266..2742)
                     /locus_tag="PLU11_05815"
     CDS             complement(2266..2742)
                     /locus_tag="PLU11_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPN58665.1"
                     /translation="MKNLRTEKAGTLLVGIGNSGRKDDGLGWKFAGLVSESANSFIDC
                     EYRYQLQVEDVLMTCRYDKIIFVDATHEVLEDGYELKPCRVAPHYYYSSHMQSPETIL
                     YLAKELYNKEPDAFTLAITGKQWGLGTSLSPEAERNLQKAWSFFEKELFQHVRITA"
     gene            complement(2723..4174)
                     /locus_tag="PLU11_05820"
     CDS             complement(2723..4174)
                     /locus_tag="PLU11_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPN58666.1"
                     /translation="MSRKITIDPVTRVEGHGRVTIHLDEYGQVKDSFFHIVEFRGFER
                     FIQGHPYWEAPVLVQRLCGICPVSHHLAAAKAIDQLVGIDPKDLSATATKLRRLMHYG
                     QVFQSHALHFFYLASPDLLFGVDAPVEKRNVVAVALENRELAIKGITMRKFGQEIIKA
                     VAGKKIHGILAVPGGVHNNLTEAERDYFLDGKEIENIDTMIEWSLAMVDFIKGYHEKN
                     RKFLDEFARFPSGHLGMVNEWGALDLYDGQLRAINSEGDITLDDVSTDLYQDYFSEAV
                     AQWSYMKFPYLKQVGRENGWNRVGPLARINVCDSVPTPLAEKARTDFIAFTGQKVNNS
                     TMYSHWARLIEVLHCAEMIKDLLHDPELMGNDLIRKGITRQKGIGIIEAPRGTLTHHY
                     EVDEKGMITRCNLIVSTTHNNDAMNTAVKWVANNVISRKGTITDGMLNQVEVAIRAYD
                     PCLSCATQAMGKMPLKAELYNYKGELINEKFKN"
     gene            complement(4186..5457)
                     /locus_tag="PLU11_05825"
     CDS             complement(4186..5457)
                     /locus_tag="PLU11_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN58667.1"
                     /translation="MAKMVNITIDGKPFQAEEGKNLMQAARQNGVFIPSLCFYEHIEP
                     PLGTCRVCTCKINGKFSPACTEKVSEGLAVEVNSPELLDTRKALVEMMFAEGNHICPA
                     CEKSGMCDLQHMGYELGVSSTRFQHLFKDRVIDFNPTRMVMEHNRCIKCLRCVVDVKT
                     RDGKKVFNYQNRGNETFVGIDYEQEARLTEDEAVNAMKICPTGAIMVRGVSQAEPFGE
                     RKYDMESVQENYNAKKKGSSRPPVMSKKIVATVSLAGCFGCHMSLLDVDEELLDIVEL
                     LSFDKSPLTDIKNFSSRCHIGLIEGGVCNSENVETLRKFREYCDILVSFGECSIWGGL
                     PTMRNSIPLSECLEEAYLNSATSEAGANIIPYHEDLPKILDRVYACNEIVKIDYYIPG
                     CPPDANHIWKAVKNILWGEEYSILYSEFKYD"
     gene            complement(5462..7201)
                     /locus_tag="PLU11_05830"
     CDS             complement(5462..7201)
                     /locus_tag="PLU11_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN58668.1"
                     /translation="MEEPRQTQLLNQLWEIQLKDGFVNDAAVQLLAKRFDLSDVEVEG
                     VVSFYHFFHRKPTGKFTIYLNNSIVSDLKGFNRIKDTFERETGASFGSVDRTGTFGLF
                     LTPCIGLSDQEPSALINFYPFTNLNAIKVKDIISQLKKGVAPEAICDAPADNIRYMPS
                     GNKTVFFKPFEAGCTLARAGQLGPEGIIEELKKAELAGRGGAYYPTWKKWNAARLQPA
                     QPKYIVCNADEGEPGTFKDRVLINTQARSLLEGMMICGYAAGAASGIIYLRGEYRWLE
                     HKLNEAILEYREAGLLGHTTKHLGNFSFDIRVQVGAGSYVCGEETALLESLEGKRGEP
                     RTKWYFPVERGYLQQPTVINNVETFCAAARILEMGADAYIQLGIPGSPGVKLLSVSGD
                     CKLPGIYEIEWGMTVAELLELCEAEDPFYIQVSGPSGECISIKDKYRRISMLDLLARK
                     DIRCGGSFMIFSKQRDLVKVLLNFTEFFKSESCGICTPCRAGNFIIQRKLERLSNGLA
                     TETDLQELKSWGTIMKNTSRCGLGKTATNSLIYAMDKFHDYFAAKLDKEFNGLNLRFD
                     MEGATKEYEQYKI"
     gene            7515..8024
                     /locus_tag="PLU11_05835"
     CDS             7515..8024
                     /locus_tag="PLU11_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN58669.1"
                     /translation="MKSDTAVPVKDAPKTDPRLKLIAARIKKENYKPDALIEILHTAQ
                     GAYGYLPIEVLTYIAKALHLPPSRVFSTVTFYHFFSLKSKGEHTCIVCTGTACYVKGA
                     QAILNKLEQQFALKPGQVTKDNKLGVQVARCIGACGLAPAVVIDNDVQGKVNVEEIIQ
                     SINEKTGIL"
     gene            8021..9661
                     /locus_tag="PLU11_05840"
     CDS             8021..9661
                     /locus_tag="PLU11_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPN58670.1"
                     /translation="MIRHELQDLTRKVKEKQQQYKHRICVCCGASCISSGSEEVLNKL
                     KEEIKARHLEDTVEVIPTGCMGPCNQGPLVKYLPEQTIYQKVNCENISQVVQSQLVDK
                     KPLEDLLLFSDSRETPFVNATEDPFFKRQQKIALRNCGDINPESIEDYLAAGGYEALD
                     KSLFILSPEEVIAEVKQSRIRGRGGAGYPTGLKWETVYKYISDQKYVICNGDEGDPGA
                     FMDRSVLEGNPHRILESMAIAGYAIGASKGFAYIRGEYPLAIKRFELAIKQATKLGLL
                     GKNILGSPFSFEVEVRIGAGAFVCGEETALIASIEGKRGTPKPRPPFPAESGLWGKPT
                     LINNVETYAAIPAIIMNGGEWYGETGTPKSAGTKVFALAGKINYSGLIEVPMGTTLRE
                     VVFDIGGGIQNGEEFKAAQTGGPSGGCIPAAHLDVKMDYESLVSLGSIMGSGGLIVMD
                     KSSDMVDVARYFMEFCMDESCGKCIPCRVGTKMMHDLLQKIVDGKATRIDLDRLEELA
                     VYVKETSLCGLGASAPNPLLSTLRHFREEYESKIVNVE"
     gene            9692..>10229
                     /locus_tag="PLU11_05845"
     CDS             9692..>10229
                     /locus_tag="PLU11_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN58671.1"
                     /translation="MNKIPVLIDEKKLEVDPHRTILEVCREHDIEILTMCHLKGIADI
                     GSCRLCLVEVEGINKLMSACTTKVTANMVISTATERIKKYQRITTELFFAERNHVCAV
                     CVSNGSCELQELGYKTGVDNIRYPHLFPQCNVDTSHPWYVMDHNRCIMCTRCVRVCNE
                     VEGAHNWDIKNRGHQVRII"
BASE COUNT         2833 a         2445 c         2253 g         2698 t
ORIGIN      
        1 ccctttaatt aaggcggcta tactcagcag tgcgaggggt aacaggatac cttccgcatt
       61 ggcatgtgcc agcaggttac tgaaagtgat ttcaccatac ccattataag caataaagaa
      121 gatagcggcc aggatagcga tacctcccac ctggttcatc cacaaggccg tcagtgcatt
      181 tttaactgat acttcatctt tcctgaatcc gataaagagg aaggaagcca gggtagtgag
      241 ttcaaagaac aggaagaaat attcgagatt atcggcggat accaccaggt tcatcgcccc
      301 gataaaaaag aagagcaggg ccaggaagaa ttttttccgg aaggcagaac agttttcttc
      361 ctcaatatac ctcagggtaa aaatagcaat gatgccgctg atgatattaa taaggagaaa
      421 catgaacgtg ctcagtttat cgaccataaa aggcatggac tccccggccg gcagattttt
      481 gagtaaatac aagagtccgg ccagttgtac aatactcatg acccctacca gccagctttt
      541 cttttgaatg gccacccagc cgaagtacag gagcatcagg atatcagcgc ctgccactat
      601 ttcatttaca tagtgcggaa cagtgaaata caggggttca ttaacatgga taaaaacata
      661 gagggccagt gcagataaga gaatagaact caggataacg attatccggg aaatggctgc
      721 cggcgcgatg aggagtattc cgccaattaa taatggaaga aaatacaatc ctgataaata
      781 gaaattcata tggcgatgaa ttttttgatt gtctaagcgg atgctatatt agaccgattt
      841 tgagtgttgg attatgatag tgatcagacc cgcgtatgat atgctgtctg ccaggaaaaa
      901 aggattgtca gcgaattgtc agttaatgct gcttttttta caccctgccc ttttcccagc
      961 agaatattgt ggcgaatact attgcctttg caacaaactg aaaaagaggc ataaaattaa
     1021 actcccggtt attagaaact atgaaaatta acccggtaat tttgtaataa ctgctatcct
     1081 ttcatccaaa cccggaacaa tggcaaagtt taccctcctg ctgtttgtcc tgtcagcctg
     1141 cctgtttttc agccagttaa ctgcccagga tctccacccg ctttatttat cagcttccga
     1201 aaaatacaag gtaaaagatt ataaaggggc tattgctgac tgtaataaac tattaaaaat
     1261 aaaaccgggt gactttgatg caacgtacct gattgcctat gcaaaatatg agcttaaaga
     1321 ttttccagct gcgctggaag acgcaaacaa actgcttaaa aaagaaaacc ccacatggac
     1381 aattgctgat ctttattatt tcaggggctt aatatacctg gagttgaacc agcaccagaa
     1441 tgcctgtaat gattttacca gaaaaattga ccagcgtcca cttctttctc catttgccta
     1501 tttcaaaaga ggtttagcca aaattaattt gaacgactat ccgggtgcaa tacaggattt
     1561 cacaaaagcc ggggtgcaat gcccgacctg tgacgaattt tttaaattta acgcgctggc
     1621 aaaggttaaa acaaatgacc tgaaaggcgc cctggccgat tataacaggc tgattgaact
     1681 aaagccggat gaaagtgagt tttacaagga aagaggcgag gtaaaatccg gtttagaaga
     1741 taacgaaggg gccatggcgg atttcaataa agccatttca attaatccga aaaacccggt
     1801 atattattat gacagggcct ccgtactgac tgcaaaagaa gattattacg gagcaataaa
     1861 agacctgcag aaagcggcag aactgaatcc ccggatcgag gaaatatata ttgcaatatc
     1921 ggataattat attggtttaa atgattttga aaaagcccta aggtatatca acatggccat
     1981 cagcgttaat actaaaaaca ttttcacctt aaatctccgg ggcttgttaa aatcaaaaat
     2041 caatgattac gcaggagctt tggcagatta caataacaac attttacttt tcccgaatga
     2101 gtggagaacc tacgtactaa gaggtaaact gaaatttgaa caccttgctg ataaagcagg
     2161 tgcctgtagt gatttcagga aagcgaaaga actcggctca gatagtgcgg cacagttaat
     2221 tcaggaaaac tgcaactgat aaaaattaat tcggaacacc gccattcagg ctgtaatacg
     2281 aacatgctgg aataattcct tttcgaaaaa tgaccaggct ttttgcaaat tcctttcagc
     2341 ttcaggacta agactggtac ctaatcccca ttgcttaccg gttatagcca gggtaaatgc
     2401 atccggttcc ttattatata gttctttggc caggtaaaga atggtttcag gactttgcat
     2461 atgggaggag tagtagtaat gcggggccac ccggcagggt tttagctcat aaccgtcttc
     2521 cagtacttca tgtgtggcat ccacaaaaat tattttatca tagcgacagg tcatcagcac
     2581 atcctccacc tgcagctgat aacggtattc gcaatcaatg aaactgttgg ctgattctga
     2641 taccagtccg gcaaatttcc agcccagccc gtcatctttt cttccgctat taccaatgcc
     2701 cacgagcagt gttccggctt tttcagttct taaatttttc attgatcagt tctcctttat
     2761 aattatacaa ctccgccttc aggggcattt tccccattgc ctgggtggca caactgagac
     2821 aaggatcata ggccctgatg gccacttcca cctggttcag cattccgtcg gtgatggtac
     2881 ctttccggct gatgacatta ttggctaccc atttcactgc tgtattcatc gcatcattgt
     2941 tgtgcgtggt ggatacaata agattacacc tagtgatcat tcccttttca tctacctcat
     3001 aatggtgggt cagtgtgccc ctgggcgctt caataatacc gatccctttc tgccgggtta
     3061 ttcctttacg gataagatca tttcccatca gctcaggatc atgtaataag tcttttatca
     3121 tttctgcaca atgcaatact tcaatcaacc gggcccagtg cgaatacatg gtactgttat
     3181 tgaccttctg cccggtaaat gcgataaagt ccgtccgcgc tttttcagcc aatggggtcg
     3241 gtacagaatc acatacatta atccgggcaa gggggccaac ccggttccag ccattctccc
     3301 ggccaacctg tttcaggtag gggaatttca tataggacca ctgggccaca gcttccgaaa
     3361 aataatcctg gtacaagtca gtggatacat catcgagtgt gatatcccct tcgctgttaa
     3421 tggccctgag ctggccgtca tacagatcca aggcacccca ttcattcacc ataccgaggt
     3481 gaccggaagg gaaacgggca aattcatcca ggaatttcct gtttttctca tgataccctt
     3541 ttataaaatc aaccatggcc agcgaccatt caatcatggt atcaatattt tcaatttcct
     3601 ttccatccag gaaataatcc ctttcagctt cggttaaatt gttatgaacc ccgcctggta
     3661 cggccagtat tccgtggatt ttttttccgg caactgcctt gataatttcc tgcccgaatt
     3721 tccgcatggt aatcccctta atggccagtt ccctgttttc cagcgcaacc gcaactacat
     3781 ttcttttctc cacaggggca tccacaccaa atagtaagtc aggcgaagcg agatagaaaa
     3841 agtgcagggc atgagattgg aatacctgcc cgtaatgcat caggcggcgc agtttggtgg
     3901 cggtggcaga aagatctttc ggatcgatcc ccacgagttg gtcaatcgct ttggccgctg
     3961 cgaggtgatg gcttaccggg catatcccgc agagccgttg caccagtacc ggtgcttccc
     4021 aatacggatg tccctgtata aaccgttcaa agccccggaa ctccacgata tgaaaaaaag
     4081 aatctttcac ttgtccgtat tcatccagat gaatggtcac cctgccgtgg ccttccacac
     4141 gggtaaccgg atcgattgta atctttctgc tcataaccaa tggaattaat cgtacttaaa
     4201 ttcggaatac aatatggaat actcttcgcc ccataaaata tttttcactg ctttccagat
     4261 atgattggca tccgggggac aacccgggat ataatagtct atcttgacta tttcattaca
     4321 ggcatatacc ctgtccagga ttttgggcag gtcttcatga tatggaataa tattcgctcc
     4381 tgcttcactg gtagctgagt tgagataggc ttcttccagg cattcactca atggtatgga
     4441 attacgcata gtaggtaacc cgccccagat cgaacactcc ccgaatgaaa ccaggatatc
     4501 acagtactcc ctgaactttc gcagggtttc cacattttca gaattacata ctcctccctc
     4561 aatcaatccg atatgacaac gggaggaaaa atttttaata tccgtcagcg gtgatttatc
     4621 aaaagacaac aactcgacaa tatccaataa ttcttcatcc acatccagca aagacatgtg
     4681 acaaccaaaa caaccggcaa gtgaaaccgt tgccacaatt tttttactca tcaccggtgg
     4741 gcggctgctt ccctttttct tcgcattgta gttctcctgt acggattcca tatcgtattt
     4801 tctttctcca aatggttcag cctggctcac tcccctgacc ataatcgccc cggtgggaca
     4861 aatcttcatt gcgttcacgg cctcgtcttc ggtcagcctg gcttcctgtt cataatcaat
     4921 cccgacaaat gtttcattcc cgcggttctg gtaattgaac acttttttcc catcacgggt
     4981 cttcacatcc accacacaac gaaggcactt gatacaacgg ttgtgttcca tcaccatgcg
     5041 ggtaggattg aagtcaatga cgcggtcttt gaacaggtgc tggaaacggg tggaggatac
     5101 acccagctca tagcccatat gctgcaaatc acacataccg cttttctcac aggccgggca
     5161 gatatgattg ccttcggcaa acatcatttc cacgagggct ttgcgggtat ccagcagttc
     5221 aggtgaatta acttccacgg ccagtccctc tgaaactttc tctgtacagg cgggactgaa
     5281 tttaccattg atcttacagg tgcataccct gcaggttccc agcggaggtt cgatatgttc
     5341 ataaaaacaa agggatggaa taaatacccc gttttgccgg gcagcctgca tcaggttctt
     5401 accttcttcc gcctggaaag gcttcccatc aattgtaatg tttaccatct ttgccatacg
     5461 atcagatttt gtattgctca tactccttag ttgccccttc catatcaaac cgcaggttca
     5521 gcccgttgaa ttccttatcg agtttagccg cgaaataatc atgaaactta tccatcgcat
     5581 aaatcaggct gttcgtggcc gttttaccca gcccgcagcg actggtgttt ttcatgattg
     5641 taccccagct ttttaattcc tgcaggtcag tttccgtagc cagcccgtta ctcagccgtt
     5701 ccagtttacg ctggataata aaatttccag ccctgcaggg cgtgcagatc ccgcaggatt
     5761 cggatttaaa aaattcggta aaattcagca acacttttac caggtccctt tgtttactga
     5821 agatcataaa ggaaccacca cagcgtatat ccttacgggc cagcaggtca agcatggaaa
     5881 tgcggcggta cttatcttta atggaaatac actcacccga tggtccgctt acctgtatat
     5941 aaaaaggatc ttctgcttca cagagttcca gtagttcggc caccgtcatc ccccattcaa
     6001 tttcatagat acccggcagt ttgcagtcac cggatacact cagcagtttg acccccggtg
     6061 agcccggtat acccagttgt atatacgcat ctgcacccat ttcaagaatg cgggccgccg
     6121 cacaaaatgt ttccacatta ttgattacag tgggttgttg taaatagcct ctttccactg
     6181 ggaaatacca tttggtccgg ggctcccctc ttttcccttc cagcgattcg agcaaggctg
     6241 tttcctcgcc gcatacataa gatccggccc cgacctggac ccggatatca aaactgaaat
     6301 ttccgaggtg tttagttgta tgtcccagca aaccggcttc gcggtattcc agtatggctt
     6361 cattcagttt atgttccagc cagcgatatt ctccccgcag gtatattata ccggatgctg
     6421 cacctgcggc ataaccgcag atcatcattc cctccaacag tgaccgggcc tgcgtattga
     6481 tcagcacccg gtctttaaat gtacccggct ctccctcatc tgcattgcaa acgatatatt
     6541 ttggctgagc cggctgcaac ctggcggcat tccatttctt ccaggttgga taatatgcac
     6601 cgcctctacc ggccagttct gctttcttca attcctcaat gatcccttct ggtcccagtt
     6661 gcccggccct ggccagggta caacccgctt caaacggttt gaagaatacg gttttattac
     6721 ccgaaggcat ataacggata ttatctgccg gcgcatcgca gatggcttcc ggggcaactc
     6781 cttttttcaa ctgggagata atgtctttta ccttgatcgc attcaggttg gtaaacggat
     6841 aaaaattaat tagtgccgaa ggttcctggt cgctgagccc gatgcagggt gtaaggaaaa
     6901 gtccaaacgt accggttcgg tccaccgaac cgaaactggc accggtttcc cgttcaaatg
     6961 tatctttaat ccggttaaag cctttcaggt cggatacgat actattatta aggtagatcg
     7021 taaattttcc cgttggcttc cggtgaaaga aatggtaaaa agaaaccacc ccttccactt
     7081 ccacatcaga caggtcaaaa cgttttgcca gcagttgcac tgctgcgtca tttacaaaac
     7141 catctttcaa ctgaatttcc caaagctggt ttagcagctg ggtttgccgg ggctcttcca
     7201 tattccgggt tttaaagtga ggtttcacca aattacgaca gcaggggtac ctgcttcaaa
     7261 cagatgccgt cataagcgga tatgattatt gtcacttttc agcgttcatt gaatcttaaa
     7321 agcccctttc cgggggattg tttaacaaca ggctccgtac tgactttaat catgtactgc
     7381 ccatacactg ctcaataatt tcgttcttgt tcatcaaagt acctccaggt acaccgcttc
     7441 atgaaagcat tatctaacga tctgccggca gctgccactg ccttttttcc cattaaacat
     7501 caggagaata atttatgaaa agcgacacgg ctgtccctgt taaagacgcg cctaaaaccg
     7561 accccaggtt aaaactgata gcggcccgga taaaaaaaga aaattacaaa cccgatgcac
     7621 tgatcgaaat actgcatacc gcgcagggtg cttacggtta tttgcccatt gaggtactga
     7681 cctatattgc caaagccctg cacctgccac catcacgggt attctccacc gttacctttt
     7741 atcacttttt ttcattgaag tcaaaaggtg aacatacctg cattgtctgc accggtaccg
     7801 cctgttatgt aaaaggagca caggccatcc tgaataaact ggaacaacaa ttcgcgctta
     7861 aacccggaca ggtcacaaag gataataaac tcggtgtcca ggtagcccgt tgtatcgggg
     7921 cctgcggtct tgccccggcg gtggtaatag ataatgatgt gcagggaaaa gtcaatgttg
     7981 aagaaatcat ccaaagtatt aacgagaaaa caggaattct atgatccgtc atgaattaca
     8041 ggacctgacc cgaaaagtca aagagaaaca gcagcaatat aaacaccgta tctgtgtatg
     8101 ttgtggtgcc agttgtatct cttcgggatc agaagaggta ttaaataaac tgaaggaaga
     8161 aatcaaagcc cgtcacctgg aagacaccgt tgaagttatt cccaccggtt gcatgggacc
     8221 ctgtaatcag gggccgctgg taaaatacct gcctgaacag actatttacc agaaagtaaa
     8281 ctgcgagaat atcagccagg tggtgcaaag ccagctggtc gataaaaagc cactggagga
     8341 tctgttgctt ttctccgatt caagggaaac accctttgtg aatgcaaccg aggatccttt
     8401 tttcaaacgc cagcagaaaa ttgcgctgag aaactgcggt gatatcaacc cggaaagtat
     8461 tgaagactac ctggccgccg gtggttacga ggcattggat aaatcgctct tcattctttc
     8521 acccgaagaa gtcatagccg aggttaaaca aagccggatc cgcggacggg gtggtgccgg
     8581 ttacccgacc ggattaaaat gggaaactgt ttataaatat atcagtgacc agaaatacgt
     8641 gatctgtaat ggtgatgaag gtgaccccgg ggcctttatg gaccgcagtg tgctggaagg
     8701 aaatcctcac cgtatcctgg aaagcatggc cattgccggg tatgcaatcg gggccagtaa
     8761 aggatttgcc tacatacgtg gtgaatatcc cctcgcgatt aaacgatttg aactcgcaat
     8821 aaaacaagcc accaaactgg gtctgttggg aaaaaatatc ctgggcagcc ctttttcttt
     8881 cgaagtggaa gtccggatcg gtgccggggc ctttgtgtgt ggggaagaaa cagcattgat
     8941 cgcctccatt gaaggaaaac gcggtacccc taaaccaaga ccacccttcc ccgctgagtc
     9001 aggactctgg ggcaagccta ccctgatcaa taatgtggaa acctatgccg ccattcctgc
     9061 tattattatg aacgggggtg aatggtatgg tgagacaggt acgccaaaaa gtgcagggac
     9121 aaaagtattt gccctggcag gtaaaatcaa ttattccggg ctgatagaag tacccatggg
     9181 caccacgctt cgtgaagtgg tatttgatat tggtggcgga attcagaacg gggaagagtt
     9241 caaagcagca cagaccggtg gtccatctgg aggatgtatc cccgctgctc acctggatgt
     9301 aaaaatggat tatgaatcac tcgtaagcct ggggtctatc atgggttccg gcggactgat
     9361 tgttatggac aaaagttcgg atatggtgga tgtggcccgt tactttatgg aattctgtat
     9421 ggatgaaagt tgcggaaaat gtattccctg ccgggtaggt actaaaatga tgcatgacct
     9481 gctgcaaaag attgttgatg gaaaagccac caggattgac ctagacaggc tggaagaact
     9541 ggccgtgtat gtcaaggaaa ccagtttatg cggactgggc gcttctgctc ccaaccccct
     9601 gctcagcacc cttcgccatt tcagggaaga atatgaaagc aagatcgtga atgtggaata
     9661 aaacaagttt actgattaca atgatgattg tatgaataag atacctgtac tgatagacga
     9721 aaaaaaactg gaggtggatc cccaccggac aatccttgaa gtctgccgcg agcatgatat
     9781 agaaatactg accatgtgcc acctgaaagg gatagcggat atcggctcct gccggctttg
     9841 tctggtggaa gtggaaggca tcaataaact gatgtcagcc tgtaccacca aagtaactgc
     9901 caatatggtg atcagcacgg ctacggaacg tataaaaaaa taccagcgga ttactacaga
     9961 actgtttttt gcagagcgaa accatgtttg cgccgtctgt gtttccaatg gcagttgtga
    10021 attacaggaa ctgggttata agaccggtgt tgataatatc cgttatcctc acttgttccc
    10081 gcaatgtaat gtagacactt cccatccctg gtatgtaatg gatcataacc gttgcatcat
    10141 gtgcacgcgt tgtgtccgtg tgtgtaacga agtggaaggc gcgcataact gggatatcaa
    10201 gaaccgcggc caccaggtgc ggatcatct
//
LOCUS       DAOPMW010000080        10218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7096_length_10218_cov_11.508413, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000080 DAOPMW010000000
VERSION     DAOPMW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_10218_cov_11.508413"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..783)
                     /locus_tag="PLU11_05850"
     CDS             complement(157..783)
                     /locus_tag="PLU11_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58672.1"
                     /translation="MITKNQLLILALLLSVSAGAQNEPKSLLVLKPSLNVGNIAIKRD
                     GGDPSSADNRVGYGIGLQFMKFLGERVFFTLGGDLNSRGYKGTRVVYLDAPVSMNVML
                     GKHKIFGFGGGAYAGMALSGKYKGTGGWTNFKFGETATDNRSRTDYGYLVNLIYQSPG
                     LFFATLTLMSGIKDITPADKQQDLNWMKLRTSQMSIVIPMSVFIKSKK"
     gene            complement(834..2564)
                     /locus_tag="PLU11_05855"
     CDS             complement(834..2564)
                     /locus_tag="PLU11_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58673.1"
                     /translation="MSLKHFLTVIVVFLFLQQADAQLGGLLKKKKDKEEKAVADTTTR
                     PDGEEKEEKKKGGGLFQKVIGKISKTAGNAVMGAGGSVANVENLGEADIIVSTGTNIY
                     SKDLGLMVTDFLGKDWINNGDFTMLQLASRDAFQMFTYPGTIKVNGKELKHASMGIHT
                     VTEPAGSGNKKVSFEKNGTVEGTFEIPVPEKNIKLLSVNGQPKNAKVDFTKDVTMELA
                     GFSTAPGSLIRVDVVGTIIGIRSLYLVCYVKPAAKITIPAVAFSHLETANKGLKYKDC
                     YLSISDQQLVKAINPTGKVPPSQMVITGSNDGMWVDVSNSRDLNMGFRLTTGNAVTEK
                     GNAAYAKPLNFAKKTAVSSFYTYGTTYLYDESTNRWTQSTTTKTIDFPEIPEAYLEGM
                     LQELYTKLTASYTEVTGHTVLPEAIVPSLPAYENTRKFFPGDINNDDEFLKAYKGLEP
                     VRTLTTVGNRYYGDGALLKEANADALLKVSLICQLSWKDKPEMTPYLKVELVGENNGD
                     FRSFAGSTKYFTMNIKGEGYELKKKAPVDFAKVFQVDELNAQFKKALAELKAKEQTIP
                     DYETIWNLQH"
     gene            2730..5699
                     /locus_tag="PLU11_05860"
     CDS             2730..5699
                     /locus_tag="PLU11_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN58674.1"
                     /translation="MQLLRKNILLLAGLLALYTADTQSLQRCKQTVYNKESGLPNQVI
                     KCITEDENGFLWMITDNYVHWFDGSVFHQVPFGKGIHQVPGIQFISIQRTPEKEIWIF
                     YDGGFSVYHPGSHTFSHYGSLIPGAAEKKFNPVAMTASELLIWTGDTIHFIHRKEKKI
                     SREMAWNRTVLLNPVPAAQLPVQILLADSARCFLFRAEKMERTSTFLPPNYGDISFHA
                     LNEKNWLFFYKDHLELVEIPGSTLLKKISYPGKKSVVNFSRPAFFLKKNENTLLVSLD
                     EQLWEFDTEKMEFSRQIVNLSGKPFFENGYFKNAFYDQRGDLWAATNLNGLLKISMQS
                     QPVQFTGTNNEQENFVKCFQVNKETNRIITGTYGKGLLVYDTTGNLLKRFMFDGPEGE
                     TNPIITTIGQIDNDQYLVFVYASANRYLLNTRTLSLRVMKQHFEKGVNYKAGSGYYSE
                     LIPLGNQQFSYNGSDRSFIFTYRNGELFCTINPPASVSGMLSSGFIKTRYNSLTGFTG
                     TPYFSQCLRKAGLTDWSATCLTKRKNNWLIGTIKGVFEFDSAQNLLHAYNRENGLPDE
                     HIYAVIIDADGNTWCSHDKGLSRIDQQGQVFNLNQQDGLQSDEFNFGAAWKAPDGQLF
                     FAGVKGLNSFYPAQLKSILDTPRIVITQISNNDLPVTDTAYWNIKQLRFSHQQNRVRI
                     RFSAIGNNPAAYYNYQYRVIGLDNSWKDLGHSLEINLALPPGEYSVEITGNHSFNPQL
                     PPQQSIRLVIVPPFYKQWWFISLAALAFLSIAWFVFRFINKRKYQEKLKALEIQAELE
                     NERQRISRDLHDNMGAYTSALLANVEKMKLQQGEQEDLDKMKGNAEQILNSLRETIWV
                     LNNKEISLADFSDGFKTYCFKLLRNFEQLEFEAEEDIADNSLLPAAVAIHLNKILQEA
                     VQNMLKHSKATVIRYHIRSLDKLEITLSDNGIGFDEATVRKGNGLSNMRWRAAEANAE
                     IVLSSSPGKGSTLQLSR"
     gene            5753..6430
                     /locus_tag="PLU11_05865"
     CDS             5753..6430
                     /locus_tag="PLU11_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58675.1"
                     /translation="MPVTIAITDDKPINRQTVKDKLAAYKEVRLVLEARNGRDFLEQL
                     KSMPALPDVVLMDLEMPELDGIETIRIATAAYPSIKFIVLTIFEDHDKIFEAIKAGAH
                     GYLLKEDSAVNIIDAITNVHEHNGIPMSPAIARKAMDLLKQIPVHHYRETEENEVVKE
                     DNAGLSDREMEILKEMVSGKNYKAIGEKLFISPLTVRKHASNIYEKLHVNSRAQAVSI
                     AHKKKWI"
     gene            complement(6440..6925)
                     /locus_tag="PLU11_05870"
     CDS             complement(6440..6925)
                     /locus_tag="PLU11_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58676.1"
                     /translation="MKRTLLLILSALLLHCICLAQEPDEEFRKFANAKIPASMQNFDF
                     FETYGTFSLRQLDTKSEVISPFSFKVSFYRNYKEVDSLSLIFFSETEEAINSAQVKTM
                     VIRFKSATGCANYLKLLGAPVSVNQWNFNMKGEQACKGVRIIKQGNKTVLVQTFSECG
                     G"
     gene            7045..7599
                     /locus_tag="PLU11_05875"
     CDS             7045..7599
                     /locus_tag="PLU11_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58677.1"
                     /translation="MSNVTKWMSWESGVDLVAVTSPELQMPNVIVHLGQLVNTPVGSA
                     PSGMIFWQPDPNAAPLVFGFVGTDAKVGAYFGPNIFAGTPFEEAPVLAATIDIQITTI
                     AAYAKCVTSDYTFEVEMSDFSEPYLINRVPAAMPPFYQQGVEIHARKATLKVNGQPID
                     IIIPPMGISGGPASVVSPNGVYAR"
     gene            7672..8268
                     /locus_tag="PLU11_05880"
     CDS             7672..8268
                     /locus_tag="PLU11_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58678.1"
                     /translation="MKKALPFLIVLCMSLPVFSQLNKEQQLIKQTFFSFLKFYQKNEN
                     AFGRFDLYQGTGPEDGPPYKINWKETEKYFAYLRKYVPYVGEAYIKAEKAHFSFSDSC
                     FKDDPAEEMPVGFDYDRWAGGQESIDYTIKWYTSPKNKYEVIITGNKALLRIGSELYP
                     GDKPENRSWSVVPFVKEKGKWKMADNVYPEDVEENPEN"
     gene            8336..9283
                     /gene="paaA"
                     /locus_tag="PLU11_05885"
     CDS             8336..9283
                     /gene="paaA"
                     /locus_tag="PLU11_05885"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HPN58679.1"
                     /translation="MPVKDFEQLFQEKIDQEIKIEPKDWMPETYRKTNIRQISQHAHS
                     EIVGMLPEGNWISRAPSLKRKAILLAKVQDEGGHGLYLYSAAETLGMSREEMINDLHS
                     GKAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQVMLTRTSYGPYARAMVRICKEESF
                     HQRQGFEALLVLSRGTAEQKAMCQDAINRWWWPALMMFGPRDTESTNSDQSMKWKIKR
                     KSNDELRQNFIDMIAEQIKTLGMHLPDPDLKWNEERKHYDFGAINWDEFWQVVKGNGP
                     CNKQRLDARRKAWEEGEWVREAAVAYARKREGRLKGEAA"
     gene            9658..10050
                     /locus_tag="PLU11_05890"
     CDS             9658..10050
                     /locus_tag="PLU11_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN58680.1"
                     /translation="MATYNHFYEMPVYKTCRAFRIKVTTFARKYFPKSEDYQLKAQLL
                     NSVRSVTANIAEGFGRFHYQENIQYCRQSRGSLTESMEHMITAFDDNFISKQLLAEIN
                     SDYKECLAALNSYIRYLKSAKLRVDNNK"
BASE COUNT         2828 a         2404 c         2369 g         2617 t
ORIGIN      
        1 taaactgtgg cctgaaaaat ttgcctgcat ccttaaaatc ccagaaccag gcgtcacgat
       61 catccagcaa ctcggttttt acctgggctg gcaaagaaat gagttgaaaa aaaagaacaa
      121 cggatagtat aatccgtctg ctaaaatttc tgttcattat ttcttgcttt taataaacac
      181 gctcatgggt attacgatgg acatctgtga agttctcagc ttcatccagt tcagatcctg
      241 ttgtttgtct gccggggtaa tatcctttat tccggacatc agggtcaggg ttgcaaagaa
      301 cagtccggga gactggtaga tcaggttgac caggtacccg taatcagtcc ggctacggtt
      361 atccgttgcc gtttccccga atttgaaatt ggtccagcca cccgtaccct tatatttccc
      421 cgacaaagcc attccggcat aggccccgcc gccaaacccg aatattttgt gcttgcccag
      481 cattacgttc atggaaaccg gcgcatccag gtataccacc cgggtgcctt tgtatcccct
      541 tgagttcagg tcacctccca gggtaaaaaa tacccgttcg cccagaaact tcatgaactg
      601 caatccgata ccatacccga cccggttatc ggccgaactg ggatcaccac catctctttt
      661 gatggcaata ttgcccacat tgagcgaagg tttcagtacg agaagtgatt tgggttcatt
      721 ttgcgccccg gcagaaactg ataacagcag ggccaggatc aatagttgat tttttgtaat
      781 catagtggct ggatcaatat tatgtattaa aaaatatttt ctggtgcatc cttttagtgt
      841 tgcaaattcc aaatggtctc gtaatccgga atggtttgct ctttggcttt taattcagcc
      901 agtgcttttt taaactgcgc attcagttca tccacctgga atactttggc gaaatcaacc
      961 ggtgctttct ttttcagctc atacccttct cctttgatat tcatcgtgaa atacttagtg
     1021 ctcccggcaa aagagcggaa atctccattg ttctctccta ccaattccac tttcaggtag
     1081 ggggtcattt ccggtttatc tttccagctg agctgacaaa tgagtgatac tttcagtaag
     1141 gcatcggcat tggcttcttt cagtaaggct ccatctccgt agtaccggtt gccaacggta
     1201 gtcagtgtcc ttacaggctc caggcctttg taggccttta aaaactcatc atcattattg
     1261 atgtcacccg ggaaaaattt ccgggtattt tcatacgcgg gcagtgacgg gacaatcgcc
     1321 tcgggcaaaa cggtatgacc cgttacttcc gtataggaag ccgttagctt agtataaagt
     1381 tcctgcagca ttccttccag ataggcttca gggatctctg gaaaatcgat ggtctttgta
     1441 gtggtcgact gcgtccatct gttcgtcgac tcatcgtata agtaagtggt gccataggta
     1501 taaaaagaac tgacggctgt tttctttgca aaattcaacg gttttgcata agccgcattc
     1561 cccttttcgg tcacagcgtt tccggttgtc agcctgaacc ccatattgag atcacgacta
     1621 ttgctcacat ccacccacat cccgtcattg cttccggtaa tcaccatttg agatggaggt
     1681 acttttcctg tgggattaat cgctttcacc agctgctgat cgctgatgga gaggtagcag
     1741 tccttatatt tcaagccttt attggcggtt tccaggtgac tgaatgccac ggccggaatg
     1801 gtgattttag cggcgggctt tacatagcag accaggtaca aggaacggat cccgataata
     1861 gtgcccacca catccacccg gatcaatgaa ccgggggcgg tggaaaaacc agccagttcc
     1921 atggttacat ccttggtaaa atcaacctta gcattcttcg gctggccatt tacagaaagc
     1981 agtttaatat ttttctccgg caccgggatt tcaaaagtcc cttccacggt tccgtttttt
     2041 tcaaaactga ctttcttgtt tccactgcct gccggctctg tcacggtgtg aatacccatg
     2101 ctggcatgct tcagctcctt cccatttact tttatggtac cggggtaagt aaacatctgg
     2161 aaggcatccc tggaagccaa ttgcaacatg gtaaaatccc cgttgttgat ccagtcttta
     2221 cccaggaaat cagtgaccat caatcccaag tcctttgaat atatattcgt accggtggat
     2281 acgattatat ccgcctcccc caggttttcg acatttgcaa cgctaccccc ggcacccatc
     2341 acggcattcc cggctgtttt agaaatttta ccaatgacct tctgaaagag gcctcccccc
     2401 ttcttctttt cttccttttc ctctccgtcc ggacgggtag tggtatccgc tacagctttt
     2461 tcctctttat ccttcttttt cttcagtaat ccccccagtt gtgcatctgc ctgttgaaga
     2521 aatagaaaaa caacgattac ggtcaaaaag tgtttcaaac tcatgcttct tatttttata
     2581 attaatcagg gttgtcaaat aacctctcca gtttacggag agtgccatag atacaaatgt
     2641 caatatcaca aacagcgcca tcaatactcc tctcagagta ttttactgca aagtcctgta
     2701 gtacagcggt catcccctat ttttgtccgg tgcagctgct gcgaaaaaac atactactac
     2761 tggcaggcct cctggccctg tatactgccg atacacaatc cctgcagcga tgtaaacaaa
     2821 ccgtgtataa taaagaatca ggattaccta accaggtgat caagtgtatc actgaggatg
     2881 aaaatggttt tctatggatg atcacggata actatgtgca ctggttcgat ggaagtgttt
     2941 tccaccaggt accttttgga aaaggcatac accaggtgcc cggcatacag tttattagta
     3001 tacagcgtac cccggaaaaa gaaatatgga tcttttatga cggcggattt tcggtctacc
     3061 atccgggttc gcataccttc agtcattacg gatcactgat cccgggagcc gctgaaaaga
     3121 aattcaatcc ggtggccatg accgcttcag agctgctgat ctggaccggc gatacaatcc
     3181 attttattca ccggaaagaa aaaaagatca gccgggaaat ggcctggaac cggacggtat
     3241 tgcttaaccc ggttccggcg gctcagttgc cggtacaaat cctgctggca gatagcgctc
     3301 gctgttttct gttcagggca gaaaaaatgg aacgaaccag tactttcctc cctcccaatt
     3361 atggggatat cagttttcat gcactcaatg aaaaaaactg gctgttcttt tataaggacc
     3421 atctggaact ggttgaaata cccggcagca cccttttgaa aaaaatcagt tatcccggaa
     3481 aaaaatcagt cgttaatttc agccggcctg cctttttttt aaagaaaaat gaaaacaccc
     3541 tgctggtttc actggatgaa cagttatggg agtttgatac tgaaaaaatg gagttctccc
     3601 ggcagatcgt caacctctcc ggtaagcctt tttttgaaaa cgggtatttt aaaaacgcgt
     3661 tttatgatca gcggggcgat ctctgggccg ccaccaacct gaatggattg ctgaagatca
     3721 gtatgcagtc gcagccggta cagtttaccg gcaccaacaa tgagcaggaa aattttgtaa
     3781 aatgcttcca ggtaaataaa gagacaaacc ggatcatcac cggcacctat ggaaagggtt
     3841 tgctggtcta tgacacgacc ggtaatctgc tgaagcggtt tatgtttgat ggtcccgaag
     3901 gagaaaccaa ccccattatc accacgattg gccagatcga caacgatcag tatctcgtat
     3961 ttgtatatgc cagtgcaaac cgttatctcc ttaatacccg gacactcagc ctccgggtca
     4021 tgaaacaaca ttttgaaaag ggtgttaatt ataaagccgg aagtggttat tattcagagc
     4081 taatccccct gggcaatcag cagttcagtt ataacggttc ggaccgcagc ttcattttta
     4141 cctaccgcaa cggagaattg ttttgcacca taaacccgcc ggcgtccgtt tcaggtatgc
     4201 tcagcagcgg tttcatcaaa acccggtata atagcctgac cggcttcact ggcacgcctt
     4261 acttcagtca atgcctgcga aaagccggac tgacagactg gtcagctacc tgtctcacca
     4321 aacgaaaaaa caactggctg atcggcacca tcaaaggagt ctttgaattt gacagtgctc
     4381 aaaacctcct gcatgcgtat aacagggaaa atgggttacc cgatgaacat atctacgcgg
     4441 tgatcattga tgcggatggg aatacctggt gcagccatga taaaggactc agtcggattg
     4501 accaacaggg gcaggtcttt aacctgaacc aacaggacgg tctgcaatcg gatgagttca
     4561 atttcggagc cgcctggaaa gcaccggacg gacagttgtt ctttgccggg gtaaaaggac
     4621 ttaacagttt ttatccggcc caactcaaaa gtattttaga taccccccgt attgtcatca
     4681 cgcagatcag caacaatgat cttccggtaa cggataccgc ttactggaat ataaagcagt
     4741 tgcgcttttc ccatcagcaa aaccgggtgc ggatccggtt ttctgccatt ggcaataatc
     4801 cggcggctta ttataattac cagtaccggg tcatcggact cgataattcc tggaaagacc
     4861 tgggccattc gctcgaaatc aatctggctc ttccgcccgg tgaatactcg gttgaaataa
     4921 cggggaatca ttcctttaat ccgcaactgc cgccacaaca aagtatccgg ctggtgattg
     4981 tgccaccatt ctataaacaa tggtggttta tcagcctggc agcactggcc tttctgagta
     5041 ttgcctggtt tgttttccga tttatcaata aacggaaata ccaggaaaaa ctaaaagcac
     5101 tggagatcca ggctgaactg gaaaatgaac ggcagcggat cagtcgcgac ctgcacgata
     5161 atatgggcgc ctatacatct gccctgctgg ccaatgtaga aaaaatgaaa ttacaacagg
     5221 gcgaacagga agacctggat aaaatgaaag ggaatgccga acagatcctg aacagcctgc
     5281 gggaaaccat ctgggtattg aataataaag agatcagcct ggccgatttc agtgatggct
     5341 ttaaaaccta ttgtttcaaa ctgctcagaa attttgaaca actggaattt gaagcggaag
     5401 aagatattgc agacaacagc ctgctgccgg ctgccgtagc cattcacctg aataaaatac
     5461 tgcaggaagc ggtacaaaat atgctgaaac acagcaaagc caccgtgatc cggtatcata
     5521 tccgcagcct tgacaaactg gagattacct tgtcggataa tggaatcggt tttgatgaag
     5581 caacggtgcg aaaaggaaat ggcctcagca atatgcgctg gcgggccgcc gaggccaatg
     5641 cggagattgt attatcttcc tctcccggca agggcagcac actgcagctc agccggtaaa
     5701 atgctcccgg cagcgtattg ttttcagtct gggaagtttt caactttgta ctatgccggt
     5761 aaccatcgcc atcacagacg acaaacccat caaccgccag acggtcaagg ataagctggc
     5821 cgcttataaa gaagtgcgcc tggtactgga agcccggaat ggacgggatt tcctggaaca
     5881 gttaaagagc atgccggcat tacccgatgt agtgctgatg gacctggaga tgccggagct
     5941 ggatgggatt gaaaccatcc ggatcgcgac cgccgcctat ccttccatca aattcattgt
     6001 gctgaccatt ttcgaggacc atgataaaat ttttgaagcc attaaggcgg gcgcccatgg
     6061 ttacctgctc aaagaagatt cagccgtcaa tattattgat gccatcacca acgtacatga
     6121 gcataacggc atcccgatga gtccggccat tgcccgcaaa gccatggatt tgctgaagca
     6181 gatacccgtg catcactacc gggagacgga ggaaaatgaa gtggtgaaag aagataatgc
     6241 cggactgagc gaccgggaga tggagatcct gaaggaaatg gtgagtggca aaaattataa
     6301 ggccatcggg gagaaattat tcatctctcc attgaccgtg cgtaaacacg cctccaatat
     6361 ttacgagaag ctgcatgtca atagccgggc gcaggcggtc agcattgccc ataagaaaaa
     6421 gtggatctga aagcagggac tatcccccac attctgaaaa agtctgtaca agtacagttt
     6481 tattgccctg tttaatgatc cggacccctt tgcaggcctg ttctcccttc atattaaaat
     6541 tccattggtt cacggaaacg ggtgccccca ataatttcag atagttcgca caacccgtag
     6601 ctgatttgaa gcggatcacc atggttttta cctgcgcact attaatggcc tcttctgttt
     6661 cactgaagaa tatcaatgaa agactgtcta cctctttgta attccggtaa aaagacactt
     6721 tgaaactaaa aggagagatg acttcagact tcgtgtccag ctgcctgagt gaaaaagtgc
     6781 cgtaggtttc aaaaaaatca aaattctgca tggaggccgg gattttagca tttgcaaatt
     6841 ttctaaactc ttcatccggt tcctgggcca ggcaaataca gtgcagcaag agtgcagaca
     6901 atatcagcaa tagcgttctt ttcatttgta tgtaagctta atgggctcaa agtgcgataa
     6961 acacccggat cagtaaatac cctctccggg gtatattcct gtggggtaaa acgttgttta
     7021 tttacagtaa cctaaaaacc ttttatgtca aacgtaacta aatggatgag ctgggaatca
     7081 ggcgtagacc tggtggccgt tacctccccc gaattgcaaa tgcccaatgt gattgtacac
     7141 ctgggtcaac tggtgaatac gccggtgggc agtgcgccca gcggcatgat cttctggcag
     7201 cctgacccga atgccgcccc gctggtgttt ggctttgtgg gcaccgatgc gaaagtggga
     7261 gcctattttg gtccgaatat ttttgccggc accccctttg aggaagcacc cgtactggcg
     7321 gccactattg atatccagat aaccaccatt gccgcctacg ccaaatgcgt aaccagtgac
     7381 tatacttttg aagtggagat gagcgatttc agcgagcctt acctgatcaa ccgggtaccc
     7441 gccgccatgc ctccctttta tcagcagggc gtggagattc atgcacggaa agctacgttg
     7501 aaagtaaatg gccaaccgat cgacattatt attcctccta tggggatcag cggtggcccg
     7561 gccagcgtgg taagtcctaa tggggtgtat gcccgataat ttgtttgcat ccgtacttta
     7621 accagcaaag agcagtttag ccgctgctct ttatttttta tttttaccag tatgaaaaaa
     7681 gcgctcccct tcctgatcgt cctttgtatg agtctgccgg tcttcagcca gctgaacaaa
     7741 gagcaacaac tgatcaaaca gaccttcttc tcctttctta aattttacca gaaaaacgaa
     7801 aacgctttcg gccgatttga cctgtaccag ggaaccggcc cggaggatgg gcccccttac
     7861 aagataaact ggaaggaaac ggaaaaatac tttgcttacc tgcggaaata tgtgccctat
     7921 gtgggagaag cctatatcaa agcggaaaag gcgcatttca gtttcagcga cagctgtttt
     7981 aaggacgatc cggccgaaga aatgcccgta ggttttgatt atgaccgctg ggcgggcgga
     8041 caggaaagca ttgattacac gattaaatgg tatacctccc caaaaaacaa atacgaagtc
     8101 attattaccg gaaacaaggc cctgctccgg atcgggtcgg aactctaccc cggcgataaa
     8161 cccgaaaacc ggagctggag tgtggtgcca tttgtgaaag aaaaaggcaa atggaagatg
     8221 gcggacaatg tgtacccgga ggatgtggag gaaaatcccg aaaactgagc ctgacctttc
     8281 aaaatttacg caaacagctg ttagttttac ttaaatttgg cccaaactgt ttgctatgcc
     8341 tgtaaaggat tttgaacaac tatttcagga gaagatcgat caggagatca agatcgaacc
     8401 gaaggactgg atgccagaaa cctaccgcaa gaccaatatc cggcagatta gccagcatgc
     8461 gcatagtgaa atcgtgggta tgctgcccga aggcaactgg atttcaagag ccccctcttt
     8521 aaaacgcaaa gccattttac tcgccaaagt acaagatgaa ggcgggcacg gactctatct
     8581 ttattctgcc gctgaaacac tgggcatgag ccgcgaggaa atgatcaatg atctgcacag
     8641 cggcaaagcg aaatactctt ccatttttaa ttaccccacc ttgacctggg ccgatatcgg
     8701 ggccattggc tggctggtag atggcgcggc catcatgaac caggtgatgc tcacccgtac
     8761 atcttacgga ccgtatgcca gggccatggt gcggatctgt aaagaagaaa gctttcacca
     8821 acgccaggga tttgaagccc tgctggtatt atcccgtggt acggccgagc aaaaagccat
     8881 gtgccaggat gcgatcaacc gctggtggtg gccggccctg atgatgtttg gtccgcggga
     8941 tacggaaagc accaacagcg accagagtat gaagtggaag atcaagcgta aaagcaatga
     9001 tgagctgcgg cagaacttta tagacatgat cgccgaacag atcaagaccc tcggcatgca
     9061 cttgcctgat cccgatctga aatggaatga agaaagaaag cattacgatt tcggcgccat
     9121 caactgggat gaattctggc aggtggtaaa gggcaatgga ccctgcaata aacaacgcct
     9181 cgatgcccgg cgtaaagcct gggaggaagg agaatgggtg agggaagcgg cggtagcgta
     9241 tgccaggaag cgggaggggc ggttgaaggg ggaggcggct tagagattat tgattattga
     9301 gagtgctata gtagggagag ttttaaagaa gattattgat tattgagagc gctatagcag
     9361 ggagagtttt aaggaggatt attgattatt gagagcgcta aagaagggag agttttaaag
     9421 agattattaa tgattgaatg agaaggggta agtgttgtaa aagccggaaa taatgttgaa
     9481 aaaacattct ttttccggaa ataaacgtaa ataaaaagtt ttttgacatg aaaggcagga
     9541 cgcctgttat tcatttttac ttattaatta ttttttcgcg tcattaggca ggagttattc
     9601 atttatcaga ctcttccaaa taattaataa ataataatta ataattttat taccaacatg
     9661 gcaacctata accatttcta cgaaatgccg gtttacaaaa cctgcagggc gttcagaatt
     9721 aaggttacta cgttcgccag aaagtatttt ccgaaatcag aagactacca gctaaaagcg
     9781 cagttactta attcagtcag atcggtaacg gcgaatattg cggagggctt tggccggttt
     9841 cattaccagg aaaatatcca gtactgccgt cagtcaagag gctcgctcac ggaatcgatg
     9901 gaacatatga tcacagcgtt cgacgataat tttatttcaa aacaattact cgcagaaata
     9961 aactcggatt ataaagaatg tctcgcggct ttaaacagtt atatccgcta tcttaaatct
    10021 gcgaaactca gagtcgataa taataaataa tcaataatta ataattttca atatgcagtt
    10081 cttacgaaaa ctatactacg gcgatatccc cgatgcccag tccggagcca cgaactctgt
    10141 gaaatcctcc ggccacgact ggcccctctg ggaagtattc atccggagta agaacggact
    10201 ggatcataaa catgccgg
//
LOCUS       DAOPMW010000081         9995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7331_length_9995_cov_9.413481, whole genome shotgun sequence.
ACCESSION   DAOPMW010000081 DAOPMW010000000
VERSION     DAOPMW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7331_length_9995_cov_9.413481"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..414)
                     /locus_tag="PLU11_05895"
     CDS             complement(238..414)
                     /locus_tag="PLU11_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58681.1"
                     /translation="MSGNHPHYEKAVGGYFLDYKYDKDGMAGIAFSFKTKLGAFHGYG
                     EKDRFDVFLTKISG"
     gene            495..938
                     /locus_tag="PLU11_05900"
     CDS             495..938
                     /locus_tag="PLU11_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58682.1"
                     /translation="MIHTDEKCLVLEKQGGNMLNPISNAVRILLSGYARIYNSRYSRS
                     GSIFRQKTKAKCLTEISLDKSGAKENLVYATTCFHYIHQNPLVAGLCKSMEDWPYSSF
                     KDYFSLNQTSLCNMDLAARYCGFHPSSFVETSLKRYNLKMERGSE"
     gene            1204..3369
                     /locus_tag="PLU11_05905"
     CDS             1204..3369
                     /locus_tag="PLU11_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPN58683.1"
                     /translation="MKKCLLTLLFLPFYLFSFSQDKILTLEDAMLRARTTLAPENLAQ
                     LQFFENSNDYVFLKKQNGQDIWMRGNYKLAGNEWPFLTLPGLNRFMEEAGSDTFKTMP
                     VIRFGTDGWIMNNKGNKIRYNPITKKITLLLSKELAAMENAEESTTGFTAYLDKHNLF
                     VSNPDKQIYQVTTDGSKDIVYASSVHRDEFGISKGIFWSTKGTKLAFYRMDQSMVNDY
                     PIINWSERPARAELIKYPMAGDKSHHVRVGVFNTATESTIWLKTGIPVEQYLTNIAWS
                     PDDKYVFIAVLNREQNHLKLNQYDATTGDFVKTLFEEQDEKYVEPLVPMHFLKNNPGQ
                     FIWQSNRDGWNQLYLYDINGKLIRQLTKGNWEVLDVKGFDSKGENLFFVSTILSAVNK
                     NLCSVNLKTGKTNLLTPLPGVHAAQVSVDGSTVLDVYSTIDNPRTIQLIEVKKPAKNP
                     AKLLLQAANPLKEFSAVSLSLFTLKNKKGDSLFCRQFNPVGFSPDKKYPVIVYWYGGP
                     HAQMILNSWNGGAGDYWFRYMAQRGYVVFTVDTRGSDNRGKNFEQSIFRKLGEAQLED
                     LDAGLSYLKAQSYVDKDRMGLFGWSFGGFLTTDYVLHNPGVFKAAVAGGPVMDWSLYE
                     IMYGERYMDTPQENPEGYASSNLINQAGKLKDKLLLIHGQQDPVVVQQHSVNFVKACV
                     EKGVQVDYMIYPGHEHNVTGRDRVHLYQKVTDYLTSQLR"
     gene            3455..4123
                     /locus_tag="PLU11_05910"
     CDS             3455..4123
                     /locus_tag="PLU11_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HPN58684.1"
                     /translation="MGIAEITQQELVIESRIITYGNAASTELTGQIREEIETLWNEPG
                     TWINIHDYYLQVVFRIAAEYRPDLTDIEIYQNLDPRNNYFRIEEFAHGNISFVDGLGC
                     NSGYFKLENLYPGSTTAAHEYGHTLGLVHPEVVDIRGKGGPGIMYPRGTLVDPEYQYE
                     PDKPAGTKGGTLHPIHRRVSDEDILLLKLHRKNFENGRAIIGDFTNIWHPNHAEIENT
                     DYLS"
     gene            complement(4111..5034)
                     /locus_tag="PLU11_05915"
     CDS             complement(4111..5034)
                     /locus_tag="PLU11_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPN58685.1"
                     /translation="MATILVTGGTGLVGKALTRQLTSMGYDCIVLSRNPGPAKDGIRY
                     AAWNPDAGTMDETSLKEADYIIHLAGAGVADQRWSVARKKEILDSRVKSGALLVNKLA
                     TLPHKVKAVISASAIGWYGADPEIPNPFPFTEEMPPDTAFLGDTCRQWEESLQPLTGM
                     HIRLVKLRIGIVLSHRGGALKEFLKPLRFGVAAVLGNGKQVVSWIHIDDLVNMFVFAI
                     TRENIEGVYNATAPVPVSNKELTITLAKTKKGFYIPLPVPAFVLKIVMGEMSIEVLKS
                     ATVSSRKIQQAGFTFTSPGIREALEKETQDK"
     gene            5132..6028
                     /locus_tag="PLU11_05920"
     CDS             5132..6028
                     /locus_tag="PLU11_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN58686.1"
                     /translation="MSIVLSIDNIAKSYGSVKALKGVSFDIPEGSVFGILGPNGSGKT
                     TLLGIVMDVLKANSGDFRWFGQPGSPEQRRKIGSLLETPNFYTYLSAIDNLKITHAIS
                     GRGNEAEINDVLKKVNLYDRRRYKFSSYSLGMKQRLAIAASLLGDPKVLVLDEPTNGL
                     DPVGIAEIRQLIIELRDKGHTIIMASHLLDEVEKVCSHVAILKTGTLITTGHVEEVLM
                     DEDIVEMSAADLAALAAALNSWGHSVTVDEKTNTVQVILPKGTASLETFNRQCFEKGV
                     VLNHLLLKKKRLEARFFELTNN"
     gene            6045..6866
                     /locus_tag="PLU11_05925"
     CDS             6045..6866
                     /locus_tag="PLU11_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN58687.1"
                     /translation="MMKLIKTEWLKIKGYNSFWWIMGLTALSYPGINYMFYFVYKDIT
                     DQKNASGQLFKMALGNPFAFPEAWHSVAYFSSFFVFIPAVVVIMFISNEYTFRTHRQN
                     IIDGWGRGQFITSKLIDVLIVTLLITLLYVAACLVSGFVTIKDGDGNNWGQAWYTGVF
                     ALQTFAQLSIAFLIGFLVRKAFIALGIFIFYFLILENILVGFFYVKVNDIGRFLPIEI
                     SDRMVPAPAFLGKIKSPEVYQESLDRIPMHVGMTIVLTTIIWLICYRINSKRDLK"
     gene            complement(6867..8246)
                     /locus_tag="PLU11_05930"
     CDS             complement(6867..8246)
                     /locus_tag="PLU11_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN58688.1"
                     /translation="MTEPVLKALAHEGYTTPTPIQQQAIPFILQGKDLLGCAQTGTGK
                     TAAFAIPILELMQAQKPLGPGIKVLVLTPTRELAIQIDESFAAYGRYAGIRHTVIFGG
                     VSQHSQVNELRRGVDILVATPGRLLDLMQQGFIHLDKLQIFVLDEADRMLDMGFIHDV
                     KKVIAKLPAKRQTLFFSATMPPEIQKLADVLLKHPAKVEVTPPSSTVDKIEQQLYFVA
                     KKEKPELLIHLLSNPEIKTTLVFTRTKHGADKVVKILQKFQIRSAAIHGNKSQNARQN
                     ALADFKNGTLRVLVATDIAARGIDIDELSHVLNFDLPDVPETYVHRIGRTGRAGNEGI
                     AISFCSADEKEELRDIQKLIGKQIPVVAEHPFTANAHMSAPAPEERPARTNFRGQQGD
                     RRQGNNRGNSGNSSRPAQRQQPARSSNSHSPSRQGSAQHQTSKHQTSQPQTPSNRQTL
                     EQWEEAERW"
     assembly_gap    8252..8351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8682..9026)
                     /locus_tag="PLU11_05935"
     CDS             complement(8682..9026)
                     /locus_tag="PLU11_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58689.1"
                     /translation="MTEEELLQHWQRLENLITAKTGALPCFDDILLYIGILESGMPPK
                     ELNEREKTDLIQMATCTVLVPARYYQLFWVEDTGWPHYKELQRLPPMPREELEQLLMP
                     WIIQYAVKNRMI"
     gene            complement(9324..>9995)
                     /locus_tag="PLU11_05940"
     CDS             complement(9324..>9995)
                     /locus_tag="PLU11_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPN58690.1"
                     /translation="AGNSTVGKMSTAGQSEEIISKPLHQSVPPIIIKETDAEMILSMK
                     DSTSSTSPVSAASGGYLARPSNIYAESKTEAITSKTSAEEPVPATKKPASIEQDELLD
                     LQMQLVERDDIPAADMPNAHQAQPPLSTKAEDPAMQDEAEEQKRRAAERLHKLRNLSF
                     NVNAADPNNEFETVPAYIRRNMELYNSNNHIESFYSSYEVKSDEKNQGHISTINTFLD
                     GKKPD"
BASE COUNT         2655 a         2209 c         2339 g         2692 t
ORIGIN      
        1 ggatacagag tgtgcttaaa agtggtcgac ctataaggaa ctaatagctc ttgcaaaagc
       61 ccctgacggt ctcgcgtttg gggcttttgc tttagtgaaa catcttagaa cgatctcgcg
      121 tttgaggctt tagcctttgt gcagcttctt agattaccaa acatcccggg gttttgcttt
      181 cgatatcacc acttttctat tcccaattgt gaattaatcc acctgtttcc tgattaatta
      241 acccgatatt ttcgtcaaaa aaacgtcaaa tctgtccttt tccccatacc cgtgaaatgc
      301 gcccagctta gttttaaaag aaaatgcaat tccagccatt ccatctttat catatttata
      361 atcaaggaaa taaccgccaa cggctttttc ataatgagga tgattaccag acatttctga
      421 atttatactt aagactcttc ccttcccgtt gttcaacgat ttcatggtgc ctgatgccta
      481 accattttca ttttatgata catacagatg aaaaatgtct ggtgcttgaa aaacaagggg
      541 gcaatatgct caaccccatt tctaatgcag tcaggatttt actaagtggt tatgccagga
      601 tatacaattc acgctatagc agaagtggtt ctattttccg tcagaaaaca aaggccaaat
      661 gtcttactga aatatctctt gacaaaagtg gagcaaaaga gaatcttgtc tatgcaacaa
      721 cttgttttca ctatatacat caaaacccgc tggtagcagg actttgtaaa tcgatggaag
      781 attggccgta ttcttcattc aaagactact tttctctgaa ccagaccagt ttgtgtaata
      841 tggatctggc tgcaaggtat tgtggctttc atccttcgag ttttgttgaa acttcgctta
      901 aaagatataa tttaaagatg gaaaggggta gtgagtaaat ggtaaatgac agacgcgaga
      961 ccggcagtca tttgcaggcg cgagattggc gaacgcaaat gacagacgcg agaccggcga
     1021 acgcaaatga cagacgcgag accgtcagtc atttgcaggc gcgagaccgg cgaacgcaaa
     1081 tgacaggcgc gagaccggcg aacgcaaatg acaggcgcga gaccgccagt catttgcagc
     1141 cggacactgt cacccattta aaaaataaga ttccctacct ttagccggac caaaacaagc
     1201 agtatgaaaa agtgcttact caccctcctg ttcctcccct tttacctgtt cagtttcagc
     1261 caggataaaa tcctgaccct cgaagatgcc atgctccggg cccgcaccac actcgcgccc
     1321 gaaaacctcg cacagttgca gttttttgaa aacagcaatg attatgtgtt tctgaaaaaa
     1381 caaaacggtc aggatatctg gatgcgaggt aattacaaac tggcaggaaa tgaatggcca
     1441 tttcttacac tgccgggact taacagattt atggaggaag ctggctccga cactttcaaa
     1501 accatgccgg tgatccgttt tggaacagat ggatggataa tgaataataa aggaaataag
     1561 atccgttata atcccattac caaaaaaatt acactgctgc tctcaaaaga actggcagcc
     1621 atggagaatg cggaagaaag cactaccggc tttacggcat atcttgataa acacaatctg
     1681 tttgtcagta atcccgataa acagatttac caggtaacta cggatggcag taaagatatt
     1741 gtctatgctt cttccgtaca ccgcgatgag tttggaatca gcaagggaat tttctggagt
     1801 accaaaggca ctaaactggc tttctaccgg atggaccaga gtatggtgaa cgattatccc
     1861 atcatcaact ggtctgaaag acccgcccgc gccgaactga tcaaataccc gatggccggt
     1921 gataaaagcc atcatgtacg ggtgggtgtt tttaatacgg ctactgaatc caccatctgg
     1981 ctgaaaaccg gaatacccgt agaacaatac ctgaccaata ttgcctggag tccggatgat
     2041 aaatatgttt ttatcgctgt actgaacagg gaacaaaatc atttgaagct gaatcaatat
     2101 gatgcaacaa ccggtgattt tgtaaaaaca ttatttgaag agcaagacga gaaatatgtg
     2161 gagccactgg tgccgatgca tttcctgaaa aataatcccg gtcagttcat ctggcagagc
     2221 aaccgggatg gctggaacca gctttatctt tatgatataa atggcaaact gatccgtcag
     2281 ctgaccaaag gaaattggga agtactggat gtaaaaggat ttgacagcaa gggcgaaaat
     2341 ttgtttttcg tcagcactat tctttctgcc gtcaataaaa atctttgctc cgtaaatctg
     2401 aagaccggta aaacgaacct gcttactcct ttacctggtg tacacgctgc acaggtaagt
     2461 gtggatggca gcacggtgct ggatgtatac agcactattg ataatccaag gacgattcaa
     2521 ctgatcgaag taaaaaaacc ggcgaaaaat ccggcaaaac ttttattgca ggccgctaac
     2581 ccgctgaaag agtttagcgc ggtatccctc agcctcttta ccctcaaaaa taaaaaagga
     2641 gacagcctgt tctgccggca gttcaatccg gttggatttt cgcctgataa aaaatacccg
     2701 gtaatcgttt actggtatgg tggcccccat gcgcaaatga tcctgaacag ctggaatggc
     2761 ggggccggtg attattggtt ccggtatatg gcacaaagag gctatgtggt ctttacagtg
     2821 gatacccggg gaagcgataa ccggggcaaa aacttcgagc aatctatttt caggaaatta
     2881 ggcgaagccc agctggaaga tctggatgcg gggctaagtt atctgaaggc acaatcctat
     2941 gtggacaaag accggatggg cttatttggc tggagttttg gcggcttcct gactactgat
     3001 tatgtgttgc ataacccggg agtgtttaaa gcggcagttg ccggcggacc ggtaatggac
     3061 tggtctttat atgaaatcat gtatggggaa cgttatatgg atacgccaca ggaaaatccg
     3121 gagggatatg ccagcagtaa cctgatcaac caggctggaa aattaaaaga caagctctta
     3181 ctgattcatg gccagcagga tcctgtggtg gtacaacaac attccgtgaa tttcgttaaa
     3241 gcctgtgtgg agaagggcgt acaggtggat tatatgattt atcccggaca tgaacataat
     3301 gtaaccggca gagaccgggt acatctgtat cagaaagtaa ccgattattt aaccagtcag
     3361 ctccggtaaa aaatcttttc ttatacatct ttccggaaag ttaaaccgtc tatagttttt
     3421 acagcataaa gacagagata cgaacagtac acctatggga attgcagaaa tcacacaaca
     3481 ggaactggtg attgaatcca ggatcatcac ttatgggaat gccgccagta ccgaactgac
     3541 cgggcagatc cgggaagaga tcgagacgct ttggaatgag ccaggcacct ggatcaatat
     3601 tcatgattat tatttacagg ttgtgttccg gatcgctgcg gaataccggc ctgacctcac
     3661 agatattgaa atttaccaga acctggatcc ccggaataat tatttcagga tagaggagtt
     3721 tgcccatggg aacatttcct ttgttgacgg attgggttgc aacagtggtt atttcaaact
     3781 ggagaattta taccccggtt caacaactgc ggcccatgaa tatggtcata ccctcggact
     3841 cgttcacccg gaagtagtgg atatacgggg aaaaggcggt cccggaatta tgtaccccag
     3901 aggcacatta gtagatccgg aatatcaata cgaaccggat aaaccagcgg gaacaaaggg
     3961 gggcaccctg catcccattc accggcgggt cagtgacgag gatatcctgc tcctgaaatt
     4021 gcatagaaag aattttgaaa acggacgggc gattatcggt gattttacaa atatctggca
     4081 cccgaatcat gccgaaatcg agaatacaga ttatttgtcc tgagtttctt tttccagggc
     4141 ttccctgata ccgggagaag tgaaggtaaa cccagcctgc tggatcttcc ggctgctcac
     4201 cgtggcgctt ttgagtactt cgatactcat ttctcccata acaatcttca atacaaaagc
     4261 cggtaccggc aggggaatat aaaaaccctt tttggttttt gccaaagtga ttgtcagttc
     4321 tttattgctt accggcaccg gtgctgtggc attgtacacg ccttcaatat tttcccgggt
     4381 aatggcaaac acaaacatat taaccaggtc atctatatga atccagctca ccacctgctt
     4441 cccattgccc agcacagcgg ctactccaaa acgaaggggt ttcaaaaatt ctttcagcgc
     4501 accaccccgg tgactaagta ctataccaat ccttaatttt acgaggcgga tatgcattcc
     4561 tgttagtggc tggaggcttt cctcccattg acggcaggta tctcccaaaa aagcggtatc
     4621 cggcggcatt tcctccgtga acggaaacgg atttggaatt tcagggtcag cgccgtacca
     4681 gccgatggcg gaagcactga tcaccgcttt tactttatgg ggaagggtgg ccagtttatt
     4741 aacgagtaac gcacccgatt ttacccggct atccagtatt tcttttttac gggccaccga
     4801 ccaacgctga tcggccaccc cggcacctgc caggtgaatg atataatctg cttccttcag
     4861 actggtttca tccatggttc cggcatcggg attccaggca gcataacgga taccgtcttt
     4921 tgcgggaccg ggattccggg acagcactat acagtcataa cccatcgatg tcagctggcg
     4981 ggtaagtgcc ttaccgacca gtccagtgcc tcctgtaacc aatattgtag ccatgttgtc
     5041 tgaaaatagt gatttaaata acataccagt caacataatg ttgcagaaga tcccccggaa
     5101 acttttatat tgagccctga aatcaacaag tatgagtata gttctgtcga ttgacaatat
     5161 cgccaaatcc tatggatcgg tgaaagccct gaaaggggtg agttttgata tcccggaagg
     5221 aagtgttttc ggcatcctcg gtcctaatgg aagtggcaag accactttgc tggggattgt
     5281 catggatgta ttaaaagcca attcgggtga ttttcgctgg tttggccagc cgggttcacc
     5341 ggaacagcgc cgtaaaatcg gatccctgct ggaaacaccc aatttctata cttatctctc
     5401 cgccatcgat aatctgaaaa tcacgcatgc catcagcggc cgtgggaatg aagcagagat
     5461 taatgatgta cttaaaaaag tgaatctgta tgacagaagg agatacaaat tcagttccta
     5521 tagtcttggg atgaaacaac gtttagccat tgccgcttca ttgcttggag atcccaaagt
     5581 gctggtactg gatgaaccca cgaatggcct ggatccggta ggtattgccg aaatacgtca
     5641 attgatcatt gaattgcgcg ataaaggaca tacgattatc atggccagtc acctgctgga
     5701 tgaagtggaa aaagtctgca gtcatgtggc aatcttaaaa acaggcacat taatcaccac
     5761 gggacatgtg gaagaagtac tgatggatga ggatatagtg gaaatgagcg cggctgattt
     5821 agcggcactg gcggctgcgc tgaacagttg gggccactcc gtcactgttg atgaaaaaac
     5881 gaataccgta caggtgatcc tgcctaaggg cacggccagc ctggaaacat tcaaccggca
     5941 gtgttttgaa aaaggagtgg ttttaaatca ccttttactg aagaaaaaaa gactggaagc
     6001 ccgtttcttc gaattaacca ataactaatc tgaatactac tgatatgatg aagctgatta
     6061 aaaccgaatg gcttaaaata aagggatata attctttctg gtggattatg ggactgactg
     6121 cgctctccta ccccggcatt aattatatgt tttactttgt ttacaaagat atcactgacc
     6181 agaaaaatgc atccggccag ctcttcaaaa tggcgctggg aaatccattt gcctttccgg
     6241 aagcctggca ttcggtggcc tatttctctt ccttctttgt ctttattccg gccgtggtgg
     6301 tgattatgtt catcagcaat gaatatactt tccgcacgca tcgtcagaat ataattgacg
     6361 gttggggacg ggggcaattc atcaccagta aactgatcga tgtactgatc gtaaccttgc
     6421 tgattaccct gttatatgtg gcggcctgcc tggtcagtgg atttgtaacc attaaagacg
     6481 gagacggcaa taactggggg caggcctggt atacgggtgt ttttgccctg cagacttttg
     6541 cccagctttc cattgccttc ctgattggtt ttctggtccg gaaagccttt attgccctgg
     6601 gtatttttat attctacttc ctgatcctgg aaaatatact ggtcggcttc ttttatgtga
     6661 aggtgaatga tatcggtcgt tttttaccga tagaaatttc tgaccgtatg gtccctgctc
     6721 cggcattcct cggcaaaatc aaatcacctg aggtttacca ggagtcactg gaccgcatac
     6781 cgatgcatgt gggcatgacc attgtattaa ccactatcat ctggctgatc tgttatcgga
     6841 tcaacagtaa acgcgacctt aaataattac cagcgttccg cttcttccca ctgctcgagg
     6901 gtctgacgat ttgaaggagt ttggggttga gaggtttgat gtttagacgt ttgatgttga
     6961 gcagatcctt gtcttgaagg ggagtgggaa ttcgaacttc ttgcgggttg ctgtctttga
     7021 gcaggtcggc tgctattacc actgttaccc ctgttattac cctggcgtct gtcgccttgt
     7081 tgtcccctga aatttgtcct tgcaggtctt tcttcaggcg caggtgcact catatgggca
     7141 ttggcggtga acggatgttc agccacgacc gggatctgtt taccaatcag tttctgaata
     7201 tcgcgcagtt cttctttctc atctgcactg cagaatgaaa tagcgatgcc ttcattaccc
     7261 gctcttccgg tacgaccgat ccggtgtaca taggtttcgg gcacatcggg caggtcgaaa
     7321 ttgagtacat gcgataactc gtcaatatca ataccacggg ccgcaatatc agtagccacc
     7381 agtactcgga gagttccatt tttaaaatca gccagcgcat tctgtcgcgc attctgcgat
     7441 ttattaccat ggatagctgc cgagcggatc tgaaatttct gcaggatctt caccactttg
     7501 tcggcgccat gtttggtgcg ggtgaatacc agagtcgttt taatttccgg attcgacaat
     7561 aaatgaatca gtaattcagg cttctctttt ttggcgacaa aatataattg ttgttctatt
     7621 ttatcaacag ttgaagatgg aggtgttacc tccacttttg ccggatgctt caataacaca
     7681 tctgccagct tctggatctc aggcggcatg gtggcggaga agaacagggt ttgtcttttt
     7741 gcgggcagtt tcgcaatcac ttttttcacg tcatgaataa agcccatatc caacatacgg
     7801 tcggcttcat ccagcacaaa aatctggagc ttatccaggt gtataaagcc ctgctgcatc
     7861 aggtcgagta aacgaccggg tgtagcgacc agtatatcca cgccacggcg aagttcattc
     7921 acctggctat gttgagatac cccaccaaaa attacggtgt gacggatacc cgcatagcgt
     7981 ccgtatgcgg cgaagctttc atcgatctgt atcgccagtt cacgggtggg tgtcagtacc
     8041 agtactttga tgccggggcc caacggtttc tgtgcctgca ttaattcaag aataggaatc
     8101 gcaaaagcgg cggttttgcc ggttccggtt tgggcgcagc caagcaggtc tttcccttgt
     8161 aaaatgaacg ggattgcctg ttgctgaatg ggggtgggtg tagtataccc ttcgtgagcc
     8221 aatgccttaa ggactggttc agtcaatgct annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nggtgtagta tacccttcgt gagccaatgc cttaaggact ggttcagtca
     8401 atgctaattt tctaaaagcc atgatggttt ttaactattt ttttatgggg ctggaaaaga
     8461 cgttgtagtt tctgaaacag cgaaagttta aaataaatct cctggtcttt ttacaaggag
     8521 agtagtccac cggcttacaa cctgccggta cctgtgtgaa gtacattgtg gagcaggatg
     8581 taaaggtaag caaaaaaaac gggatagcgg gaaatgattc ataatgcacc gttaaaccac
     8641 tcttattcag tatacttaac tatagcaatc gttaaggctc gttaaatcat cctgttcttt
     8701 actgcatatt gaatgatcca tggcattagc aattgctcca gctcttccct tggcatcggt
     8761 ggcagccgct gtagttcttt ataatgcggc cagccggtat cttccaccca gaacagctga
     8821 taataacggg cgggcacgag tacagtgcag gtagccattt gtataagatc agtcttttcc
     8881 ctttcgttta actctttggg aggcatacct gattcgagta tacctatata taataagata
     8941 tcatcaaaac aaggaagtgc tcctgtttta gctgtgatca ggttttcaag tcgttgccag
     9001 tgctggagaa gttcttcttc ggtcatcagc agcgaaggta gaatgccggg atgttggccc
     9061 ctatgatttt ggttgggatg gtgggaaaga gaaatcagtt atatcccggc gtccttttgc
     9121 ctgcggcatt gtagtaatat tatgtcccgg cgtccttttg cctgcggcaa aagagacacc
     9181 gggacataat aaaaaccctc cggtagaaac cggagggaac aactaaaaaa cactagctaa
     9241 atgctcgtgc cgcccggtgg acccggggcg gacggggctt cattggaacg aacaacgggt
     9301 gccggatggg tacggcaaaa tctttagtcg ggtttcttcc cgtctaagaa ggtattaatc
     9361 gtgctgatat gaccttgatt tttttcgtca gatttgactt cgtaactgct gtagaaactc
     9421 tcaatatggt tattgctgtt atacaattcc atattgcggc ggatataagc cggcacagtt
     9481 tcaaactcat tattggggtc agcagcattc acattgaatg acaagtttct caacttgtgc
     9541 aaacgctccg ctgccctacg tttttgctcc tctgcttcgt cctgcatagc aggatcctca
     9601 gccttggtgg acaaaggtgg ttgagcctga tgggcattcg gcatatccgc cgctggaatg
     9661 tcatcccgct ctacgagctg catttgcaag tccaggagtt cgtcctgttc tatactggca
     9721 ggcttttttg tagccgggac cggttcttcg gcagaagttt ttgaggtgat tgcctctgtt
     9781 tttgattccg cgtagatatt ggacggtctg gccagatatc ctcccgatgc tgcagacacc
     9841 ggggatgtac tactagtact atctttcata ctgagaatca tctcagcgtc tgtttcttta
     9901 ataataatgg ggggaacgga ttggtgcagc ggtttcgaaa ttatctcttc gctttgtccc
     9961 gcagttgaca ttttgcccac agtagaatta cctgc
//
LOCUS       DAOPMW010000082         9981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7345_length_9981_cov_8.138525, whole genome shotgun sequence.
ACCESSION   DAOPMW010000082 DAOPMW010000000
VERSION     DAOPMW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_9981_cov_8.138525"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(122..280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(394..939)
                     /locus_tag="PLU11_05945"
     CDS             complement(394..939)
                     /locus_tag="PLU11_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58691.1"
                     /translation="MFGLFKKKNNSDSVTVTDKVVISEPAKLTVLLEAWNNQPQSVIV
                     CWFEESLEKITAYMNEHSSVTVPVLLVREALTAQRAGRTPLFAEHYPLHSKEQEAYQS
                     MHLEKAVVYSSLREPLFSQFGGEKIIKVMEQLGMKETELIEHSMISSAIRKAQEKIEQ
                     KTPLDHLARSQEEWIEKNYKP"
     gene            complement(1073..1648)
                     /locus_tag="PLU11_05950"
     CDS             complement(1073..1648)
                     /locus_tag="PLU11_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58692.1"
                     /translation="MKKTIVIMGGFVLTLLSVTVSAQQTWDAKKNPTVDSISALYRDK
                     ILTAPAPPTREDVFPVIGKYESATNVDAALLSITLDEVNKGIAWIDGLPQGRVKAMLR
                     KSPATYKIPAQKTAEGNDVAEGTLIYDKLTNTLSICLGKEYNAENPAAAFVPVEEPAV
                     VTVKNAKSKKPVTPKAWMYTGTKMSKETAMN"
     gene            1845..4697
                     /gene="uvrA"
                     /locus_tag="PLU11_05955"
     CDS             1845..4697
                     /gene="uvrA"
                     /locus_tag="PLU11_05955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPN58693.1"
                     /translation="MAKSKQPEITPVVQEPETIEVYGARVHNLKNIDISIPKNKLVVI
                     TGISGSGKSSLAFDTIYSEGQRRYMETFGAYARQFIGDMERPDVDKITGLSPVISIEQ
                     KTTNKNPRSTVGTVTEIYDFLRLLYARIGEAYSYNTKKRMVKWSEEEIVDNIFKKYKG
                     KKIALLAPMVRGRKGHYRELFEDVRKKGFLKVRVDGEIKDLVPKMQVDRYVIHDIELV
                     VDRLQVTDELRARLSQSVQQTLKLGKDLMFMLVDDTKFVQFSKLLMCEDTGISYEEPS
                     PNAFSFNSPYGACPVCKGLGTKHHINMDAIITDPTLSISEGGIAPLGEERDAWVYRQA
                     QEAAKKNKISLTNPVSKLTQKQLNIMFYGNEDGQQEDIEFENAKFDPNAPFEGVINML
                     QRWFANPYSEAIREWAEDYMIVKPCDSCNGTRLKKESLWFKVNGRNIAELSQMNLDNL
                     AAWFDGIELMLDNKQQAIAKDVLKEIRERLQFLLDVGLTYLTINRSSRTLSGGESQRI
                     RLATQIGSQLQGITYILDEPSIGLHQRDNHRLIEALKNLRDIGNSVLVVEHDKDIMLA
                     ADHLIDIGPKAGFHGGRIVAQGDPKDLLKLDTLTSGYLNGHHRIEIPAERRQGTGKFL
                     ELKGAAGNNLRKVDVKFPLGKFICVTGVSGSGKSTLINETLYPILSKHAFGSKMTPLE
                     YKSIKGLELIDKVIEIDQSPIGRTPRSNPATYCGFFTEIRTLFASLPESKIRGYNAGR
                     FSFNVKVGRCDVCEGGGMRVIEMNFLPDVYVHCEKCNGKRYNRETLEIRYKGKSIADV
                     LAMTVEEAVEFFQPVPYLFRKIKVLEEVGLGYITLGQSAVTLSGGEAQRVKLSTELAK
                     RDTGKTFYILDEPTTGLHFEDIRHLLVVLNKLVDRGNTVLVIEHNMDVIKVADHVIDI
                     GPEGGDGGGRVLFEGTPEELVKNKESFTAQYLKMEL"
     gene            complement(4744..5358)
                     /locus_tag="PLU11_05960"
     CDS             complement(4744..5358)
                     /locus_tag="PLU11_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HPN58694.1"
                     /translation="MNLIERAKNIMLTPAKEWDVVNGEEANVPGIITGYVLPLAGAAA
                     VAAFIGYGLIGANLGFGVRLKGIDWGLYHGISVLAGALLSVFVSAFVIDALAPSFGSE
                     KNLGKSVQLVAYSWTPGWVGGLLAIIPSLAIIGGLASLYGLYLLYLGLPKLKKTPQDK
                     QVSYFVVSLLVMIVVFVVIGLIMSKIFMSVFGLSYGLGNASFSL"
     gene            complement(6088..6513)
                     /locus_tag="PLU11_05965"
     CDS             complement(6088..6513)
                     /locus_tag="PLU11_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58695.1"
                     /translation="MKPLFNVRSLAGLVILTGLLIAEPAFASGTPYRKPITVSNNPEE
                     EEPAKKEKGKSRNFTSLNNSAVKIHPAAVKREMHVVAKENDGAEVDFFVFDLQGTIIQ
                     HYKMKPKSHYKLSGLRKGKYTFSVFQGDAETASGKFEIR"
     gene            complement(6540..7493)
                     /locus_tag="PLU11_05970"
     CDS             complement(6540..7493)
                     /locus_tag="PLU11_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58696.1"
                     /translation="MRTSLLKVRLSALVCFLAVFSFSSQAQSFTTGNGKVEIGLGIGP
                     MFFLGDLGGSAGVGKTFIKDLDLPLTKLNKGLYVNYYPTEWFGLRLAGNLGYVSGDDA
                     QAPSKGGAEMDRRERNLNFRSKISEAYVAAEIYPTYFLEKYDGLLGKLRPYLLAGVGI
                     YHFNPETKDVDGSWVKTAPLRLEGQGFAEYPDSKPYKLTQKNVLGGFGVKYYVKENMY
                     LGFEILHRKLFTDYVDDVSNNYYIDPIYFDQYLPAADAVKARRLYYRGTYSFPTTRPY
                     EEFAERGDPKQNDAFFSSIFRLGWRLGGNSNLKHLKCPVFY"
     gene            complement(7636..8091)
                     /locus_tag="PLU11_05975"
     CDS             complement(7636..8091)
                     /locus_tag="PLU11_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN58697.1"
                     /translation="MTTWTLKKFDELTPHELYAILQLRSEVFVVEQNCVYQDMDNKDI
                     SAWHLMGWDNETPDKKLLAHTRLLPPGLSYPEASIGRVVSSPAVRGKGIGRELMQKSI
                     ETIYILYGAGPIRIGAQLYLKSFYVSLGFQPEGEIYLEDNIPHIIMYLP"
     gene            complement(8254..9681)
                     /locus_tag="PLU11_05980"
     CDS             complement(8254..9681)
                     /locus_tag="PLU11_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN58698.1"
                     /translation="MLSSPILSVNNLSITRSGKKILENISFSLYNGEALAITGPAGCG
                     KTSLGLALAQQLYYQGTITYHFPDGSPIAWVEQQHHFKTLQSTSDLYYQQRFNSYDTE
                     ETQTVAESLGQQLSAARELIAEMGMAYLLEEKLIQLSNGENKKLQLLNALLGKPAVLI
                     MDQPFTGLDTGTRLWLNEQIIRLKAAKVLVMMISSADEIPESADKVLFLENGYMQSIE
                     DKAGLLRKPVVVETKQEPPAAETIPLSKEHPVFNSIIDMEEVTVRYGDKLILDRINWR
                     VLPGECWLLTGPNGAGKSTLLSLVNGDNPQAYANKIHLFGQRRGSGESIWDIKKKTGF
                     LSPELHVYFNDSSTGFETVASGLFDTIGLFRQVSAEDTAWVMQWMEVCGISTHKNKRL
                     LELSTGEQRMLLLARALVKDPPLLILDEPCQGLDPDRKHRLLELINRVCTVTGKTLVF
                     VSHYEADVPGCVDKFIRIEQGRRVS"
     gene            complement(9811..>9981)
                     /locus_tag="PLU11_05985"
     CDS             complement(9811..>9981)
                     /locus_tag="PLU11_05985"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPN58699.1"
                     /translation="YGKEYQYSHSYESNFSPQEYLPKELAGKAFYQPGKNAREEELRK
                     FLKGLWKDKYGY"
BASE COUNT         2645 a         2243 c         2342 g         2751 t
ORIGIN      
        1 gagagcgggt ggaccgggag gcaatacccc gtcgctaaag ctatggggta taaaaaaagc
       61 tcacccgata aaacagatga gcttttgaat atcttgaaga ctatttagaa gctatatatc
      121 tggcttatct ttcccgtttg cacgggatgg agttggcacc ttttccactg tcatcctgag
      181 catcattgtc atcctgagtt tatcgaagga tgcgaaggaa ggagtggaga ggttgtcaag
      241 gcatcatcgg gccattaccc tctgcctttc ttgataagta cattaaagaa ctgctgcaaa
      301 tatacaacag gtaggtaatt taaaaaacaa aaaccgattc aagaggcaaa cggttgttcg
      361 ctattccttc aaagcccttt gtggtcaagg gtttcagggc ttataatttt tttcaatcca
      421 ttcttcctgg gagcgggcca gatgatccaa tggagttttt tgctcgatct tctcctgggc
      481 cttccgaatg gcactgctga tcatactgtg ctcaatcagt tctgtttcct tcatacccag
      541 ctgttccatc actttaataa ttttttctcc gccgaactgg ctgaacagtg gttcacgtaa
      601 ggaggagtaa acaacagctt tttcaagatg catggactgg taagcttcct gttctttact
      661 atgtaaggga taatgttcgg cgaaaagggg tgttctgccg gcccgttgtg cggtcaatgc
      721 ctcacggacc agtaatacag gtaccgttac agaactgtgt tcattcatat aagccgtgat
      781 tttttcgaga ctttcctcaa accagcaaac gatcacggat tgcggctgat tattccaggc
      841 ttccagcaat acagtcagtt tggcgggctc actgatcacc actttgtcgg ttaccgttac
      901 cgaatcagaa ttgtttttct ttttgaataa accaaacatg gggcgaattt gctgctaaag
      961 tagaatgcag ggggggagca accaaattat tttaacgtat acaaaaaagg ccaccgctct
     1021 tacaagcggc agccttttct gttactgtgt atgaaaaccg tcctttagtt gtttaattca
     1081 ttgcggtctc tttggacatt ttagtgccgg tatacatcca ggctttcggg gttaccggtt
     1141 tttttgattt agcattttta acggtaacaa cagcgggttc ttctaccggg acaaatgccg
     1201 cagcagggtt ttccgcattg tattctttac ccagacaaat actcagggta ttggtcagtt
     1261 tatcgtaaat cagggtgcct tccgctacgt catttccttc cgcggttttc tgagcgggta
     1321 ttttataggt tgccggtgat ttcctgagca tggctttcac gcgaccctgg ggcaatccat
     1381 cgatccaggc gattccctta tttacttcat caagagtaat gctgagaagg gctgcatcca
     1441 cattggtggc agactcatat ttcccgatca ccgggaatac atcttcacgg gtagggggtg
     1501 ccggggctgt taggatctta tcgcgataca acgccgaaat agaatctacc gtgggatttt
     1561 tctttgcatc ccaggtttgc tgggcactga ctgtaacgga aagtaaggtc aatacaaatc
     1621 cgcccattat aacaatggtc tttttcataa gagaaatttt taatgattta attggtactc
     1681 gggcattact tcaaaatatt gtgccagagt aaatatttgt ggataacttc aaaaactttg
     1741 gctctttttc cctgtttttg atggagttac tggcggaagg gtcaatcatc tccatcacta
     1801 tataaagagg gagccccgct gaatcagtat cttcgtttcc cgatatggca aagagtaaac
     1861 aaccagaaat cacccctgtg gtgcaggaac ccgagaccat tgaagtatat ggggcccggg
     1921 tgcataacct gaaaaatatt gatatatcca tccccaagaa taagctggta gtgatcaccg
     1981 gtatcagtgg cagtggcaaa tcctcccttg cttttgatac catttattcg gaaggtcaac
     2041 gccggtatat ggaaactttc ggcgcttatg cccggcagtt tattggtgat atggaacggc
     2101 cggacgttga taagatcaca ggtctgtctc cggtgatttc cattgaacaa aagaccacga
     2161 ataaaaatcc ccgctccacc gtaggcacgg tgacggaaat ctatgatttt ctccgcttgc
     2221 tctacgcccg tatcggggag gcttactcct ataataccaa aaagcggatg gtgaaatgga
     2281 gtgaggagga aatcgtcgat aatatcttta aaaaatataa ggggaaaaag atagccctgc
     2341 tggcccccat ggtccgggga cgtaagggcc attaccggga actttttgaa gatgtacgca
     2401 aaaaaggatt cctgaaagtg cgtgtggatg gggaaataaa ggacctggtt cccaaaatgc
     2461 aggtcgaccg gtatgtgatc catgatattg aactggtagt ggaccggctg caggtaaccg
     2521 atgaactcag ggccaggctg agccagagtg ttcaacaaac cctcaaactc ggcaaggatc
     2581 tgatgtttat gctggtggac gatacaaaat ttgtccagtt ttccaaattg ctgatgtgtg
     2641 aggataccgg tatcagctac gaagaacctt cccccaatgc tttttcgttc aactctccgt
     2701 atggggcctg tccggtctgt aaaggactgg gtacaaagca ccatatcaat atggatgcga
     2761 tcattaccga ccccaccctg agtatcagtg aaggggggat tgccccgctg ggagaagaac
     2821 gcgatgcgtg ggtttaccgc caggcccagg aggcggcgaa aaagaacaag atttccctga
     2881 cgaacccggt aagtaagctg acgcaaaagc agctgaacat catgttctat ggaaatgaag
     2941 acgggcagca ggaagacatc gaatttgaaa acgccaaatt cgatcccaat gccccctttg
     3001 aaggagtgat caatatgctg caacgctggt ttgccaatcc atacagtgaa gcaatccggg
     3061 agtgggcaga ggactatatg attgtaaaac cctgtgacag ctgcaatggc acccgtttga
     3121 aaaaagaaag tctctggttt aaagtcaatg gccgcaatat tgccgaattg agtcagatga
     3181 atctcgacaa cctcgcggcc tggtttgatg gcatcgaact gatgctggac aacaagcaac
     3241 aggctattgc caaggatgtg ctgaaggaaa tcagggaacg gttgcaattt ttgctcgatg
     3301 tgggactgac ttatctgacc atcaatcgtt cttcccgcac gctcagtggg ggagagtcac
     3361 aacggatacg gctggctacc cagattggtt cccagttaca ggggatcacg tatattttag
     3421 atgaaccttc gatcggattg caccaacggg ataatcaccg cctgatcgag gcattgaaaa
     3481 atctccgcga tattggaaac agtgtgctgg tggtggaaca cgataaggat attatgctgg
     3541 cggcggatca cctgattgat atcggaccca aggccggttt ccatggcgga cggatcgtgg
     3601 ctcagggtga tcccaaagat ttattaaaac tggataccct gacttccggt tacctgaacg
     3661 gccatcatcg gattgaaata ccggcagagc gtcggcaagg aacgggaaaa ttcctcgagt
     3721 tgaaaggtgc cgccggaaat aacctgagga aagtggatgt gaaatttcca ttgggaaaat
     3781 ttatctgcgt gaccggcgtg agtggaagtg gtaagtctac cctgatcaat gaaaccttat
     3841 atcccatctt gtccaaacat gcatttggct caaaaatgac cccgctggag tataaatcca
     3901 tcaagggcct cgaactgatt gataaagtaa ttgagattga ccagtcgccg attggccgaa
     3961 ctccgcgaag taatccggcc acctattgcg gattttttac cgagatccgg actttgtttg
     4021 cctctcttcc ggaatccaaa atacggggat acaatgcagg tcggttctcc tttaatgtaa
     4081 aagtgggccg ttgtgatgtg tgtgaaggcg ggggcatgcg ggtgattgaa atgaatttcc
     4141 tgccggacgt atatgtccat tgtgaaaaat gcaatggcaa acgctataat cgtgaaacac
     4201 tcgagatccg ctacaaaggg aaatccattg cggatgtact ggcgatgacg gtggaagaag
     4261 cggttgaatt tttccagccg gtgccctacc tcttcaggaa gatcaaagtg ctggaagaag
     4321 tgggactggg atatatcacg ctcggacagt cggccgtaac actcagcggt ggtgaagccc
     4381 aacgggtcaa attatccacg gaactcgcca aacgggatac ggggaaaacc ttttatattc
     4441 ttgatgaacc aaccacggga ttgcactttg aagatatccg tcacctcctg gtggtattaa
     4501 ataaactggt ggaccgcggc aacacggttt tggtgattga acataatatg gatgtgatca
     4561 aagtggccga tcatgtgatt gatattggtc cggaaggcgg cgatggggga ggaagggtgt
     4621 tgtttgaagg aacgccggag gagctggtga aaaataagga gagctttacc gcacaatacc
     4681 tgaaaatgga attataaaaa aaggaccagc attgttgctg gtcctttttt attcagctat
     4741 ttattataag ctaaaacttg cattgcccaa tccatagctt aggccaaata cagacataaa
     4801 tatctttgac atgattaaac ctataaccac aaataccaca atcatgacga gcaatgaaac
     4861 tacaaagtag ctgacttgtt tgtcttgtgg tgttttctta agtttaggta aacccaggta
     4921 aagcaggtac aatccgtaaa ggctggcgag tcctccgata atggcgagtg aaggtatgat
     4981 tgccagcaat cctcccaccc atccgggggt ccaggaatat gccaccagtt gtacagattt
     5041 acccaaattc ttttcggaac caaaactggg agccagggca tctattacaa atgcgcttac
     5101 aaacacactc agtaaagcac ctgcaagaac agaaattccg tggtacagac cccagtcaat
     5161 acccttgaga cggacgccga atccaaggtt agcacctatt aagccatatc cgataaatgc
     5221 agcaactgcg gctgctcctg ccaggggtaa tacatatccg gtaatgatgc ccggcacatt
     5281 tgcttcttct ccatttacga catcccattc tttagcgggt gtcagcataa tgttttttgc
     5341 tctttcaatt aagttcataa ttggttgttt ttggttgaag acctactcat atggcttttc
     5401 ggcattcgcc cccggttcag gctccgggtc gccttactgc ttatggaact aatttaagct
     5461 agtttctgca taaatgagaa tgaaacaaag ctaattgttg ataaccggtt aacatatccc
     5521 tgttttaggg gaatctgaag acaaagtacg aagttagaag tacgaagtac gaacgggatt
     5581 tcgtgaagcg agttactata cgttatttaa ggttggactt tcttagaagt ggctgttatt
     5641 gccgactgcc gactgccgac tgccgactgc ctactgcata ctgcctactg ccaactaatc
     5701 ccccctgtaa acaacaaaag ccactaaaca gcggcttctc gtacttcgta cctcgtactt
     5761 cgtacttgtt gattccctta aggtaaaggc aggaagtgat tgtaaaaatc ttcagcttag
     5821 gggttcctgg ttttgccggg atactggatt tagacaattt ccctgcctgg ttacctggat
     5881 tagaaataat ccggataata ccttttcggg tttcttagtt atgacaatta aaagagctca
     5941 gggttgataa attcccgaaa gcagggcaga gagcatttaa taaaattgta tacaaatgag
     6001 agtaccaata gagaacggat taggtttatt caaccaggta tcagatttta ctaatctccc
     6061 tgccgctttt cgggacggca ttcgttttta acgtatttca aattttccgg aggcggtttc
     6121 cgcatctccc tggaaaacac taaatgtata ttttcctttg cgcaggccac tcagcttata
     6181 atgactcttc ggtttcattt tataatgctg gatgatggtg ccctgcaggt caaatacaaa
     6241 gaagtctact tcggcgccgt cattttcttt ggctaccacg tgcatctcac gttttacggc
     6301 agccggatga attttgacag ctgaattatt cagggaagta aaattccggg atttgccttt
     6361 ttctttttta gccggttctt cttcttccgg attgttgctt acagtaatgg gtttacggta
     6421 aggggtgccg gaggcgaatg ccggttcagc aatgaggagg ccggtgagga tcaccagtcc
     6481 cgccaggctg cggacattaa ataagggttt catagtggtt cggtattagg cggtacggat
     6541 cagtaaaaca ccgggcattt caggtgcttc aggttactgt tgccaccgag gcgccagcca
     6601 agccggaaaa tactggagaa gaaagcgtca ttttgtttgg gatcgcctct ttcagcaaat
     6661 tcttcatagg gccgggtggt cgggaagcta taagtacccc tgtagtataa ccttcttgct
     6721 tttaccgcat cagcggcagg gagatactga tcaaaataga tcggatcgat atagtagttg
     6781 ttactcacat catctacata atcggtaaat aatttacggt gcaggatctc gaatccgaga
     6841 tacatgtttt cttttacata atattttaca ccaaaacctc ccagcacatt tttctgggtc
     6901 agtttgtaag gtttactgtc ggggtattcc gcaaatccct gtccttcgag acggagtggg
     6961 gcggttttca cccagctgcc atccacatct ttggtttccg gattgaaatg atagataccc
     7021 acacccgcca gtaaataggg gcggagtttg ccaagtaagc catcatattt ttccaggaaa
     7081 taagtaggat aaatttcagc agccacatag gcttcgctga ttttggaacg gaaattcaga
     7141 ttcctttccc ggcggtccat ttctgcacca cctttggaag gggcttgtgc atcatcgcct
     7201 gaaacataac cgaggttacc ggcaagacgt aaaccgaacc attcagtagg gtagtagtta
     7261 acgtacagcc ctttgttcag tttggtcagt ggtaaatcaa ggtctttaat aaatgtttta
     7321 cctacacctg cacttccacc caggtcgcca aggaagaaca tcgggcctat gccaagaccg
     7381 atttcaactt tgccattacc tgttgtgaat gattgtgcct gtgaggaaaa agagaagact
     7441 gcaaggaagc agactagcgc ggacagccgc actttgagta aacttgttct cattggtatc
     7501 agatttctgg tttttctact attggatttc gagaactaag gtagatgaat agtaaaatac
     7561 agggctaata tttgagccgt tatttttgtt tttgaccaga attaaagcgc ttctacttcg
     7621 tgtttttacg aagactcagg gtaaatacat aatgatatgg gggatattat cctcaagata
     7681 aatctcgccc tcaggttgaa aacccagcga tacatagaag gattttaaat ataactgagc
     7741 tccgatacgg atcggtcccg ctccgtataa tatatatatt gtctcaatcg atttctgcat
     7801 cagttcacgg ccaatccctt ttccacgaac agcaggggag cttaccaccc ggccaataga
     7861 tgcttccgga tacgacaggc ccggaggtaa taaacgcgta tgcgccaata actttttatc
     7921 gggcgtttca ttatcccatc ccatcaggtg ccaggcggat atatctttat tgtccatatc
     7981 ctggtacacg caattctgtt ccaccacaaa aacctcactt cttaactgaa gtattgcata
     8041 aagttcatga ggcgtgagtt cgtcgaattt cttgagagtc caggtggtca tttaacaggt
     8101 gttttgacaa agatagggaa gagagagagt ccacagttgg cagtccacag tccacagtaa
     8161 acagccaatt ctaagaaggt tatatcttaa gtaacaaata gtaactcgtt ttacgaaatg
     8221 ccgctcgtac ttcgtacttc taacctcgta cttctaactc acccgtcttc cctgctcaat
     8281 cctgataaac ttatctacac atcccggtac gtctgcttca tagtgcgaaa caaataccag
     8341 tgtcttaccg gttacggtac agacccggtt gatcagttcc agcagccggt gctttctgtc
     8401 cgggtccagt ccctggcagg gttcatccag tattaataag ggtgggtctt ttaccagggc
     8461 cctggccagc agcagcatcc gttgttcacc ggtggagagt tccagcagtc gtttattttt
     8521 gtgtgtactg atcccgcaaa cctccatcca ttgcatgacc catgctgtgt cttcagcgct
     8581 tacctgccgg aacagtccga tggtatcaaa aagtccggaa gctaccgttt caaaaccggt
     8641 ggaagaatcg ttgaagtaga catgtagttc aggtgacagg aaaccggttt tcttcttgat
     8701 gtcccagatg ctttccccgc tccctcttcg ctggccaaat aaatgaattt tattcgcata
     8761 ggcctgcggg ttatccccgt ttaccagact taataatgtt gatttccccg ctccattcgg
     8821 acctgttagc agccagcact ctccgggtaa tacgcgccag ttgatccggt ccaggatcag
     8881 tttgtcgcca taccggacgg tcacttcctc catatcgata atactgttaa atacaggatg
     8941 ttcctttgac aaaggaattg tttcagcagc agggggttcc tgtttggtct ctacaacaac
     9001 cggcttccgg aggagtccgg ccttgtcttc tatggattgc atatacccgt tttccaggaa
     9061 gagtacttta tccgcgctct ccggaatttc atctgccgag ctgatcatca ttaccaatac
     9121 ttttgccgct ttcagtctta tgatctgttc gttgagccac aggcgggtgc ctgtatccag
     9181 tccggtaaag ggctgatcca tgatcagtac cgccggtttc cccagtaagg cattcagtaa
     9241 ctgcagtttt ttattttctc cgttggataa ttggatgagc ttctcctcca gcagataggc
     9301 cattcccatt tcagctatca gttcccgggc cgccgacaac tgctgtccca atgattcagc
     9361 cacggtttgg gtttcttccg tatcatagga attaaaccgc tgctggtaat acaaatccga
     9421 ggtggattgc agggttttaa aatgatgctg ttgttccacc caggctatcg gactcccatc
     9481 aggaaaatga taggtaatcg taccctggta atataattgc tgagccagcg ccagtcccag
     9541 gctcgtcttg ccacatccag ccggcccggt aatagccaga gcctcaccgt tgtaaagaga
     9601 aaatgaaata ttttccagga tcttttttcc tgaacgtgtg atcgaaagat tgtttactga
     9661 taagattggg gatgataaca taaaaaaaag aatagctacg agctgcgagt tacgagctag
     9721 attttgtaaa acgagttaca atttgttatt ttagatttaa ccctcttaga atgggctgtt
     9781 gactactaac taccgactac tatctactaa ctagtatccg tacttatcct tccacagtcc
     9841 cttcaaaaac ttcctcagtt cttcctcccg tgcatttttc cctggctgat aaaaagcttt
     9901 tccggctaat tctttgggca ggtattcctg cggggagaaa ttgctctcat aactgtgcga
     9961 atactggtat tcttttccat a
//
LOCUS       DAOPMW010000083         9956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7365_length_9956_cov_6.993233, whole genome shotgun sequence.
ACCESSION   DAOPMW010000083 DAOPMW010000000
VERSION     DAOPMW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7365_length_9956_cov_6.993233"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..265)
                     /locus_tag="PLU11_05990"
     CDS             complement(5..265)
                     /locus_tag="PLU11_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58700.1"
                     /translation="MKKILSAIIALYLIVPTAKAQDDEIRPKAIGVSFFVNDFITPDR
                     IRTTSLSQVISAKRFAKIREMAPGLAVTYFKGLKPHIDIAAL"
     gene            complement(421..2109)
                     /locus_tag="PLU11_05995"
     CDS             complement(421..2109)
                     /locus_tag="PLU11_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPN58701.1"
                     /translation="MIRKTLYPIVYFLSATFFLACTGNGQPADTVASSPEELKIKERQ
                     LIEYYLQKAVGDSGRTEDTLVRLFSPELLRSFYTADSNNVYWSAGELWLPEADSLYRF
                     IKEGRLLGLYPEDYHYQELQMIRDRLFADSLGKGDRKDARWWSAADLLLTDALARMIK
                     DVKLGRLPQDSISLRKDSTLSDSFYLAHISAVKKQGMAAVIQALEPTARGYQLLKAAV
                     PEFLSRANFTQTYSPVPYPEKNSSRFNQALQKRLFEEGLMTNDSIPADSTQLAAAVKS
                     YQKKMGITVDGRAGEGTVRMLNTDDREKFARIAISMDKYKQLPAKMPSRYIWVNASAN
                     YMEVIDSNKLKIRSKVITGKPKTRTPVLTSLISTIITYPQWVPPPSIVQKEILPAVKK
                     NPGYLAKKGFSLVDKDGNEVDPYSVDWSKYSKGIPYRVVQGSGDANALGIIKFVFDNK
                     YSVYLHDTNQRYLFANAMRSLSHGCVRVQEWDKLVWYILSSDSILASGRGTVKIDSVK
                     SWLRNKQKRNVSLKHKIPVFIRYITCEGQNGSLVFFDDVYGEDKFLRQRFFKGK"
     gene            complement(2317..2616)
                     /locus_tag="PLU11_06000"
     CDS             complement(2317..2616)
                     /locus_tag="PLU11_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HPN58702.1"
                     /translation="MPVIYHVTTAGDWEAARQNGFYTHPSLGAEGFIHCSQENQVAGV
                     LERYFEGQANLVKLVIDTDRLTSRFVFDWSPSTQDTFPHIYGPINLDAVTEVLTL"
     gene            complement(2616..3608)
                     /locus_tag="PLU11_06005"
     CDS             complement(2616..3608)
                     /locus_tag="PLU11_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPN58703.1"
                     /translation="MNSFGRLFRISIFGESHGESVGITIDGCPAGLSLQAEDLLPDLE
                     RRKGGIQKGTTPRQETDFPIFKSGLFNNKTTGFPLTILFENNNTRSEDYNKQRSIPRP
                     GHADWVANQKFGGHEDYRGGGHFSARLTTGLVAAGAIAKKLMPELRIEAKVTEIGGEK
                     DTEKGLQNAIEAKDSVGGIVECLVTGLPVGLGEPYFDSLESVLAHILFAIPAVRGVEF
                     GTGFAAAKMYGSAHNDAIEDMTGKTRTNHAGGVVGGISNGNELVFRLAVKPTSSTSKT
                     QNSLNWETGTTEDFSIKGRHDLCVALRAPVIVEAVTAIVLADCMLLEQKIKRVL"
     gene            complement(3618..4913)
                     /gene="aroA"
                     /locus_tag="PLU11_06010"
     CDS             complement(3618..4913)
                     /gene="aroA"
                     /locus_tag="PLU11_06010"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPN58704.1"
                     /translation="MVVTIHPSRLTGSIQAPASKSSMQRACAAALLHKGETHIHNPGH
                     SNDDKAALDIIERLGASIDDRGELLIIHSKGIHPVKEDISCGESGLSIRMFTPLAALS
                     KKQVTINGTGSLLTRPMDFFDEILPQLGVSIKSKEGKLPLVLKGPLVPQTIEVDGSLS
                     SQFLTGLLMAYSAAGASHVSIKVNNLKSRPYIDLTLEVMKQFGMNLPVNKSYTEFFFG
                     DAPVVKKTAIPINYTVEGDWSGGAFLLVAGAIAGPVTIRGLDPGSTQADKAILEALMS
                     ANAGLAIDAKGITIHPTAMKGFAFDATDCPDLFPPLVALAAYCEGLTTIKGVSRLAHK
                     ESNRGLTLQEEFEKMGVRIELDDDHMRVYGNGRVNGAKVHSRHDHRIAMACAVAALRA
                     GGDVTIEQANAVKKSYPDFFRDLKKLGGTVSLPFESFNF"
     gene            complement(4915..5934)
                     /gene="aroB"
                     /locus_tag="PLU11_06015"
     CDS             complement(4915..5934)
                     /gene="aroB"
                     /locus_tag="PLU11_06015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPN58705.1"
                     /translation="MKYRKYKFSASSTDFYFAGGISQLRKITDSKSTILLTDENVYKA
                     HTKRFRGWNTIVLKAGEDYKVQATVDAVVETLIEMEADRKTTLVGIGGGVITDLTGYI
                     ASVYMRGIRFGFIPTSVLGLVDASIGGKNGIDVGQYKNMVGVIRQPSFILHDMIFLKS
                     LPQQEWENGFAEVIKHACIKDATMFAALENSSLMTYLKNQKSSCALVERNALIKIKVV
                     QKDEFEKADRRLLNFGHTLGHALETQYELLHGQAISIGMTYACHISEELTGFRQTDRV
                     VSLLEKYHLPTYAAFDKQKVFQVLKMDKKRERAEMNYVLLDRIGKGVVKSITLKKLEQ
                     ILQKL"
     gene            complement(6008..7096)
                     /locus_tag="PLU11_06020"
     CDS             complement(6008..7096)
                     /locus_tag="PLU11_06020"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPN58706.1"
                     /translation="MQTTEQTSNAAAQAAWNKRPLIISGPCSAETEEQVLATAQRLAA
                     TGKVDMLRAGIWKPRTKPGMFEGVGAKGLPWLQQAKKITGLPTTVEVATGKQVQDALT
                     FDVDVLWIGARTTVNPFSVQEVADALRGVDVPVMIKNPINPDLELWGGAVERVARAGV
                     KQIGLIHRGFSSYGNSEFRNAPMWHLAIDMKLKYPGLPLINDPSHICGRRDILLDVMQ
                     RAIDLDYDGLMIESHIDPDNAWSDAKQQITPERLAEMLDQIIWRKEDVNSEEYHAALE
                     ALRQKINHLDDEMMQILGQRMKIAEQIGQYKKDNNITILQTGRWNEIIERASAKGEKI
                     GLSKEFITRYLDAVHMESINHQKKVLDS"
     gene            complement(7161..8009)
                     /locus_tag="PLU11_06025"
     CDS             complement(7161..8009)
                     /locus_tag="PLU11_06025"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPN58707.1"
                     /translation="MERKKIAIVGVGLIGGSLAIQLHEKKLSSRLIGVESSSEHAAQA
                     LDLELVDEVLPLEAAIAAADVIILAIPVDSMVLLLPSVLDKIDQQMVLDLGSTKSQLV
                     DVISNHPKRGRFCATHPMWGTEYSGPAAAVKGAYENKAVILCNPEQTDEDVLEWVKHM
                     FTKIGMRQLVMEAKAHDLHAAYVSHISHITSFALANTVLEKEKEENAIFELASAGFES
                     TVRLAKSNPAMWVPIFLQNKVNILDVLNEHIEQLKKFRDSIRDDKTEALRTLIEDANK
                     IRRIIK"
     gene            complement(7999..9174)
                     /locus_tag="PLU11_06030"
     CDS             complement(7999..9174)
                     /locus_tag="PLU11_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN58708.1"
                     /translation="MKVAKRLEGIGEYYFSQKLREIEELNKQGKNIINLGIGSPDQPP
                     HPEVIRVLQEEAAKPHVHAYQPYKGSMVLRLAISDWYHRWYGVQLNPDAEVLPLIGSK
                     EGIMHICMTYLNKGDEALLPDPGYPTYTSAVKLAGGKPVNYTLREKNNYEPDFDKLEK
                     KGLKKVKLMFVNYPQMPTGQLPAKELFEKLVAFGKKHQILIVHDNPYSFILNDDPLSL
                     LSVEGAKETAVELNSLSKSHNMAGWRMGMLCGAKERIDEVLRFKSNMDSGMFLPLQLA
                     AAKALSLGKEWQEEVNTIYRQRKEKAIELLQLLGCRYSRKQAGLFLWARVPAGFKDGY
                     AASDEVLYGANVFITPGGIFGKGGNQYIRVSLCATVEKFEEAIHRIKTKMENTNYGA"
     gene            complement(9319..>9956)
                     /locus_tag="PLU11_06035"
     CDS             complement(9319..>9956)
                     /locus_tag="PLU11_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPN58709.1"
                     /translation="NYNLLQKSNLKIAGEIYLQIRQNLLVNPGVQLEDIREVHSHTMA
                     LQQCYDFLDKYKWKLVETDDTALSAKNIHQHKSKHVAAIASKLAADLYELDVLAPNIH
                     TMKNNYTRFLMLEREETVLPVTDADKASLTFHTDHSKGSLVKVLSRIADGGINLSKLQ
                     SFPIPGTDFKYAFHADMEFDNREQFEKVLKEIAPLTEAVKVYGVYKRGLWK"
BASE COUNT         2433 a         2555 c         2131 g         2837 t
ORIGIN      
        1 tatttcaaag ggctgcaata tcaatatggg gtttcagccc tttgaaataa gtaactgcca
       61 gtccgggtgc catttcgcgg atcttcgcaa aacgtttggc gctgattacc tggctcaatg
      121 aggtggtacg tattctatcg ggagttataa agtcgtttac aaagaaacta acaccaatag
      181 ccttcggcct gatttcatca tcctgggcct tggctgtagg cacgatcaga tacaatgcaa
      241 taatagcaga taatatcttc ttcataaaga gatagtttgg ttttaatcac ggcaaaaata
      301 actggtatgt ttaatatcct aaaataaatt atttagttag tattcatcgg tttggagggt
      361 cttttttttg aatttccaga gatttctccg ggtgggcggc ttatatcaga ccggtttgtc
      421 ttatttaccc ttgaaaaagc gctgacggag aaatttgtcc tccccatata catcatcaaa
      481 aaataccagc gatccgtttt gcccttcaca ggttatataa cggataaaaa ccggtatctt
      541 atgttttaaa gacacattcc ttttctgctt gttccggagc caggatttca ctgaatcaat
      601 cttaactgtg ccccggccgg aagccagtat actatcacta ctcagaatat accagaccag
      661 tttatcccac tcctgcaccc tgacacaacc atggctcaat gaccgcatgg cattggcaaa
      721 caagtagcgc tggttggtat catgtaagta aacagaatat ttattatcga acacaaactt
      781 tatgattccc agtgcattag cgtcgccact cccctgtact acccggtagg gtataccctt
      841 cgagtatttt gaccaatcca ccgaatacgg atccacttcg ttgccatcct tgtccaccag
      901 actaaacccc tttttagcca gataacccgg attttttttg actgccggta atatctcttt
      961 ctgtacaata cttggcggcg gcacccattg cggataagta ataatagttg aaatcaggct
     1021 ggtcaaaacc ggtgttcggg tttttggctt tcctgtaatg acttttgatc tgattttcaa
     1081 tttattacta tctattacct ccatataatt tgcggatgcg ttaacccaaa tatatcggga
     1141 gggcattttc gcgggaagct gcttgtattt atccatactg atcgcaatcc gggcaaattt
     1201 ttcccggtcg tctgtgttca gcatccggac cgtcccctct cctgcccggc catcaacggt
     1261 gattcccatt tttttctggt agctttttac agcagcggcc agttgtgtgg agtctgccgg
     1321 aatgctatcg ttggtcataa gcccttcctc aaaaagcctt ttctgcaatg cctgattgaa
     1381 acgactggaa tttttctccg ggtaaggaac cggcgaatag gtttgggtaa aattggccct
     1441 ggatagaaat tcaggcactg cggctttcag taactgatag ccccttgctg tgggctccaa
     1501 agcctgtatc acagcggcca tcccctgttt ttttacagca ctgatatgcg ccaggtaaaa
     1561 actgtctgat agtgtggagt ctttccggag actgatgcta tcctggggta gtctgcccaa
     1621 ttttacatct ttgatcatcc gcgccagggc atctgtcagt agaagatcag ccgccgacca
     1681 ccaacgtgca tctttccggt cgcctttccc aagactatcc gcaaaaaggc gatcccggat
     1741 catctgcaat tcctgataat gataatcttc gggatacagc cccaataacc gtccctcttt
     1801 tatgaaacga taaagtgaat ccgcttccgg cagccaaagc tcgcctgcac tccagtaaac
     1861 attattgctg tcagccgtat aaaaggaacg aagcaattcc ggggaaaata aacgaaccag
     1921 cgtatcttct gtacgtccgc tgtccccaac tgctttttgc agatagtatt ctatgagctg
     1981 tcgctcttta attttgagct cttccggact ggaagcgact gtatcggcag gttgtccatt
     2041 gcccgtacag gcaagaaaaa aagttgccga taaaaaatag acaatcgggt acaatgtctt
     2101 tctgatcata aaggactggc gggtggcctg tttaatggtt ctggactaaa atagctgccc
     2161 aggagttttc gtatgcacta caaaatagtc aaaggtttca acattcaccg aatcatttga
     2221 ttgatttttt taattgccct gaaccagctg acaagtgcag gcaaaaccgg ctcctgagcg
     2281 gaagaagcgg aaaaaatggc tttaccagta aagaaactag agagtaagca cttctgttac
     2341 ggcatccaga ttgatcggac cataaatatg cggaaaggta tcctgtgtgg agggcgacca
     2401 gtcaaacaca aaacgtgagg tcagccggtc agtatcaata acgagtttga ccaggttggc
     2461 ctggccctca aaataacgtt ccagcacccc agccacctgg ttttcctgag aacagtgaat
     2521 aaacccttca gcaccgaggg aaggatgtgt ataaaatcca ttttgcctgg cggcttccca
     2581 atccccggcg gtggttacat gataaatcac tggcattaca atacgcgttt aattttttgc
     2641 tccagcagca tacaatctgc cagtacaatg gccgtgactg cttctacaat cacaggtgcc
     2701 cgtaaagcta cacaaagatc atgacgacct ttaatggaaa aatcttcagt ggtaccggtc
     2761 tcccagttca ggctgttttg tgtttttgaa gtggaagaag taggtttcac ggcaaggcgg
     2821 aaaaccagtt catttccatt actgattcca cctaccacgc caccggcatg gttggtgcgt
     2881 gtcttacccg tcatatcctc aatcgcatca ttatgtgcac tgccatacat ttttgccgcg
     2941 gcaaacccgg ttccgaattc aactccacgc acggcaggga tggcaaaaag aatatgcgcc
     3001 agtactgatt ccaacgaatc aaaataaggt tctcctaatc ccactggcaa tccggtcacc
     3061 aggcattcca caatgccccc caccgaatcc ttggcctcga tggcattctg taatcctttt
     3121 tccgtatcct tttctcctcc aatttcggtc accttagctt ctatgcgaag ctccggcatt
     3181 aacttttttg ctatggcacc agccgccacg agtcccgtgg tcaaccgggc actgaaatgt
     3241 ccgccacccc ggtagtcttc gtgcccgcca aatttctgat tggccaccca gtccgcatga
     3301 cccggacggg gtatactgcg ttgcttattg taatcctcac tccgggtatt attattttca
     3361 aagagaatcg tcagaggaaa accggtcgtc ttattattga aaagaccact tttgaaaata
     3421 gggaaatcag tttcctgacg gggagtggtc cctttttgta tacccccttt tcttcgttca
     3481 agatcgggta ataaatcttc cgcctgcagg gataatccgg ccgggcaacc atcaatagta
     3541 atcccaactg actcaccatg tgattcacca aaaatggaaa tccggaaaag tcggccaaat
     3601 gaattcacgt gtataattta gaaattaaag gactcaaagg gtaaagatac agtcccaccc
     3661 agttttttca agtcgcggaa aaaatccgga taggattttt tcaccgcatt ggcctgttca
     3721 atagttacat ccccacctgc ccttaaggct gcgaccgcac aggccatcgc gatccggtga
     3781 tcatgccgtg aatggacctt tgccccgtta acccggccat tgccatatac ccgcatatga
     3841 tcatcatcga gctcgatccg gacacccatc ttttcgaatt cttcctgaag ggtgagcccc
     3901 cggttacttt ccttatgggc cagccggcta acccctttaa tggttgtcag gccctcacag
     3961 tatgcggcca gggctaccaa tggaggaaac agatccggac aatcagtggc atcaaatgca
     4021 aagcctttca tagcggtagg gtgtatcgtg atacctttcg catcaatcgc cagtccggca
     4081 tttgcggaca tgagtgcctc caatatggct ttatctgcct gcgtggaccc cggatcaagc
     4141 cccctgattg ttaccggccc ggctattgct ccggcgacca gcaaaaaagc cccgccgctc
     4201 cagtctcctt ccaccgtata atttatgggt atggccgttt tttttaccac gggggcatct
     4261 ccgaagaaaa attcagtata ggatttattt accggtaaat tcatcccaaa ctgtttcatc
     4321 acttccaggg taagatcgat atagggccgg cttttcaggt tattgacttt tatagaaaca
     4381 tgactggccc cggctgctga gtaagccatc agtaaacccg tcaggaactg ggaactgagt
     4441 gaaccatcca cttcaatggt ttgtggcaca agcggcccct ttaatacaag cggaagtttc
     4501 ccttccttgc tttttattga tacgcccagt tgtggcagta tttcatcaaa aaaatccatc
     4561 ggccgggtaa gcagactgcc tgttccatta atggtgacct gctttttact caaagccgcc
     4621 agcggggtaa acatccggat gctgagtccg ctctctccgc aactgatatc ttcctttacc
     4681 gggtgaatgc ccttgctgtg tataatcagc aattcccccc ggtcatcaat agacgcgccc
     4741 agtcgttcta tgatatcaag ggccgcttta tcatcattgc tgtgtccggg attatgaata
     4801 tgggtttccc ctttatgaag taaggccgcg gcacaggccc gctgcatcga acttttggag
     4861 gccggtgcct ggatactgcc ggttaaccgg gatggatgga ttgttacaac cactttataa
     4921 tttttgcagg atctgttcca gcttttttaa tgtaatagat ttcacaactc ctttaccgat
     4981 acggtccagc agcacataat tcatttcagc ccgctccctt tttttgtcca tcttcaatac
     5041 ctggaagact ttttgtttgt caaatgcggc ataagtaggc aaatggtatt tttccagcag
     5101 gctgaccacg cggtcggtct gacggaaacc ggtcagttct tcggaaatat gacaggcata
     5161 agtcatacca atcgatatgg cctggccatg gagtaattca tattgcgtct cgagtgcatg
     5221 acccagtgta tgcccgaagt tcagcaggcg gcggtccgct ttttcaaatt catctttctg
     5281 caccactttt attttgatca gggcattacg ttccaccaat gcgcagctgc ttttttggtt
     5341 tttcaggtag gtcatcaggc tgctgttctc caaagcggca aacatggttg catctttgat
     5401 acaggcatgt ttgatcactt ccgcaaaacc attctcccat tcctgctgag gcaatgattt
     5461 caggaaaatc atatcatgca ggataaaaga tggctgacgg ataaccccga ccatattttt
     5521 atactgtccc acatcaatcc cattcttgcc tccgatagaa gcatccacca gtccaaggac
     5581 agatgtcggg ataaaaccaa aacggattcc acgcatatac accgaagcaa tatagcccgt
     5641 caggtcggtt atgactcctc ccccgatccc caccaacgtg gttttccggt ccgcttccat
     5701 ttcaatcagg gtttccacca ccgcatccac cgttgcctgt actttatagt cttccccggc
     5761 tttcaataca attgtattcc atcccctgaa tcgttttgta tgggccttat aaacattttc
     5821 atcggtgagg aggatcgtgg atttagaatc cgtgatcttc cggagctgac tgatcccacc
     5881 ggcaaaataa aaatccgtgg aagaagctga gaatttgtat ttcctgtatt tcattttcaa
     5941 attcaataaa cattacaaag tccggctgtc tttgactacc gactaccgac tccggactac
     6001 cgactgatta actatccagc accttcttct ggtgattgat actctccata tgcacggcat
     6061 ccaggtagcg ggtgataaac tctttgctga gcccgatctt ttcccctttg gcactggctc
     6121 tttcaatgat ttcattccag cggccggtct gcaggatggt gatattatta tcttttttat
     6181 actggccgat ttgttccgca attttcatcc gctgacccag tatctgcatc atctcatcat
     6241 cgaggtgatt gatcttctga cggagggcct cgagggcggc atggtattct tcagaattca
     6301 cgtcttcttt tctccagata atctggtcca gcatttcagc cagtcgttca ggagtaatct
     6361 gctgtttggc atcactccag gcattgtcag ggtcaatatg actttcaatc atcagcccgt
     6421 cgtagtccag gtcaatggcc cgctgcatca catccagcag aatatcgcgt cggccacaga
     6481 tatgagaagg atcattgatc agaggaagac cagggtattt cagtttcata tcaatcgcca
     6541 ggtgccacat tggggcattc cggaattcgg agttaccata agaactgaaa ccacgatgga
     6601 tcagtccgat ctgttttaca ccggcacggg ctacccgctc cacggctcca ccccataatt
     6661 cgagatccgg gttgatcggg tttttaatca tcacgggtac atccactcca cggagggcat
     6721 cagctacttc ctgtacactg aaagggttaa ccgtggtacg ggcgccaatc cataatacat
     6781 ccacatcaaa tgtaagggca tcctgtacct gtttaccggt agccacttct accgtggtag
     6841 gcaaaccggt tatttttttg gcctgttgca gccagggcaa acctttggcc cccactcctt
     6901 caaacattcc aggtttagta cggggtttcc agattcctgc acggagcata tccactttac
     6961 cggtggcagc gagccgttgg gcagtggcga gcacctgttc ttcagtttcc gcactgcagg
     7021 gaccactgat gatcagggga cgtttgttcc aggcagcctg tgctgccgca ttacttgtct
     7081 gttcggttgt ttgcattgtt caaagttagt gctccctgtt cctattcggg taccggcagc
     7141 ctttttttat tgaatgttgg ttatttaata attcttctga ttttattggc gtcttcgatg
     7201 agtgtccgga gcgcttccgt tttatcatca cggatactgt cccggaactt tttcaattgt
     7261 tcgatatgtt cattcagcac atccaggata ttcactttat tctggaggaa gatcggcacc
     7321 cacatggccg gattactttt ggccagtcgt actgtacttt caaaaccggc cgaagccagt
     7381 tcgaaaatgg cgttctcttc cttttctttt tccaggacgg tattggccag ggcaaaagaa
     7441 gtgatatgtg aaatgtgact cacataagcc gcatgcaggt catgggcttt tgcttccata
     7501 acgagttgcc gcattccaat ctttgtaaac atatgtttta cccattccag cacatcctca
     7561 tctgtttgtt ccggattgca aaggatgacc gctttgtttt catacgcgcc ttttaccgcc
     7621 gcggccggac cactgtactc agtgccccac atcgggtggg tggcgcaaaa ccggccccgc
     7681 ttcggatgat tgctgatcac gtccaccagc tgcgatttag ttgatccgag atccagcacc
     7741 atctgctggt caattttatc cagcacagat ggcaataata ataccatgct gtcgacagga
     7801 attgctaaaa taataacatc cgctgctgca atggccgctt ccagtggcag tacttcatcc
     7861 accagttcaa gatcaagcgc ctgggctgca tgttcggaac tggattccac gccaatgaga
     7921 cgggaactca gttttttctc atgcagctgg atggccaggg agcccccgat gagtcccacg
     7981 ccaacgatgg caattttctt acgctccata attagtattc tccattttag ttttaatccg
     8041 gtggatggcc tcttcaaatt tctccaccgt tgcgcatagg cttacccgta tatattgatt
     8101 ccctcctttc ccgaaaatac cgccgggtgt aataaataca tttgctccgt acagcacttc
     8161 atcactggcg gcatacccat ctttaaaacc ggccggaacc ctggcccata agaataaacc
     8221 ggcctgcttc cgggaatacc ggcatcccag caattgcaat aattcaatcg ctttttcctt
     8281 tcgctgtctg tatatcgtat ttacctcttc ctgccattct ttccccaatg aaagggcttt
     8341 ggcggctgcc agttgaagtg gtaaaaacat tccgctgtcc atattgcttt tgaaccgcaa
     8401 tacttcatca atccgttctt tcgctccgca aagcatgccc atccgccagc cagccatatt
     8461 gtgcgatttg ctcagcgagt ttaactccac ggctgtttct tttgcaccct ccacggataa
     8521 gagactcagc ggatcatcat tcagaataaa agagtatgga ttatcatgca caatgaggat
     8581 ctggtgcttt ttaccaaatg ccaccagttt ttcaaacagt tccttagcag gcaactgccc
     8641 tgtaggcatc tgtgggtaat tcacaaacat cagtttcact ttcttcaggc ctttcttttc
     8701 caatttgtca aaatccggtt cataattatt tttctccctg agcgtataat taaccggttt
     8761 accaccggcc agtttaaccg cactggtata ggttggatag cctggatcgg gcaacaaagc
     8821 ttcatctcct ttattcagat aagtcataca gatatgcatg attccttctt tactgccgat
     8881 cagcggcaat acttccgcat cgggattaag ctgcaccccg taccagcgat gataccagtc
     8941 gctgatggcc agccgcaaaa ccatggaccc tttgtagggc tggtaagcat gtacatgagg
     9001 cttcgcggct tcttcctgca atacgcggat tacttccgga tgcgggggct ggtccggact
     9061 gccgatcccc aggttgataa tattctttcc ctgtttattc agctcttcga tctcccgcag
     9121 tttctgcgag aaataatact ccccgatgcc ttccagtctt tttgccactt tcataatgat
     9181 gtagtttgtc tattattgtg aattgaacat tgagcacctg cctgccggca aaggcaggtt
     9241 gaatattgag cgttttgtct tcttagtagt tcaatgaaca atattcaacg ctcattattc
     9301 aatgttcatt tgtaagaact atttccaaag cccacgttta tataccccat acactttcac
     9361 cgcttcggtc agcggagcaa tttccttcag cactttttca aactgctccc ggttatcaaa
     9421 ttccatatcc gcatgaaagg catatttgaa atcggtacca ggaataggaa aactctgcag
     9481 cttgctgaga ttgatacctc catcagcaat acgggacaat actttcacca ggctaccctt
     9541 ggaatgatct gtgtgaaagg taagggaagc tttgtctgca tctgtcacgg gcaatacggt
     9601 ttcctcccgc tccagcatca ggaagcgggt ataattattc ttcatggtat ggatattcgg
     9661 tgccagtacg tccagttcat agaggtctgc ggccagttta ctggcaatgg ccgccacatg
     9721 tttgctctta tgctggtgaa tatttttggc actcagcgcc gtatcatccg tctccacgag
     9781 tttccattta tacttatcga gaaaatcata acactgttgc agggccatag tatgtgaatg
     9841 aacttcccgg atatcttcca gttgcactcc gggattcacc agcaggtttt gccggatttg
     9901 cagatagatc tccccggcaa ttttaaggtt gctcttttgc aataaattat aattcg
//
LOCUS       DAOPMW010000084         9950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7371_length_9950_cov_7.548156, whole genome shotgun sequence.
ACCESSION   DAOPMW010000084 DAOPMW010000000
VERSION     DAOPMW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7371_length_9950_cov_7.548156"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLU11_06040"
     CDS             <1..839
                     /locus_tag="PLU11_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN58710.1"
                     /translation="KKPIGSFIFLGTTGVGKTELAKALAEYLFDDESMMTRIDMSEYQ
                     EKHTVSRLVGAPPGYVGYEEGGQLTESVRRKPYSVVLLDEIEKAHPDVWNVLLQVLDD
                     GRLTDNKGRVVNFKNTIIIMTSNIGSHLIMDAFDGIKEKDIEEANAKAKVEVMNLLRQ
                     TIRPEFLNRVDEIIMFQPLLKREIMGIVKIQLNSLKKLVAENGITLQFSDYALEFLSD
                     QGFDPQFGARPLKRLIQKEIVNQLSKRILAGDIDKSKPVLVDVFDGVVVFRNELPEKV
                     KG"
     gene            1025..1735
                     /locus_tag="PLU11_06045"
     CDS             1025..1735
                     /locus_tag="PLU11_06045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPN58711.1"
                     /translation="MTQQEEKTTITSMPRIGDPAPHFEAVTTQGNISFPEDYKGKWVI
                     LFSHPADFTPVCTSEFMTFAKLEADFNRLNTQLVGLSVDGLYSHIAWLRTIKERIEFR
                     DMKNVEVKFPLIEDISMNVATKYGMIQPNESSTKAVRAVFFIDPKGIIRAMIYYPLSL
                     GRNFKELKRVIIGLQTADKHAVALPADWQPGEDVIVPPANSCGAAADRMAGKDEGVYC
                     KDWFFCMKPLAKEEVEMA"
     gene            complement(1786..2949)
                     /locus_tag="PLU11_06050"
     CDS             complement(1786..2949)
                     /locus_tag="PLU11_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN58712.1"
                     /translation="MQHLKSQFLLRDDITFLNFGSFGACVKPVFEKYQQYQLELEQEP
                     VLFITGNGPKYLEQSREALGQYLHADKDDLVYVTNPSFAVNTVARSLKLKTGDEVLTT
                     NLEYGACDKTWNYYCEQTGARYVRQPISLPLTSKEEFVDELFKGVSPKTRLIFISHIT
                     SSTALRLPVEEVIIKAKTLGIPVFVDGAHAPGQVSLNLTTLGVDYYTGACHKWMLTPK
                     GSSFLYVKKEKQPAIDPLVISWGYKAATPSSSTFLDYHQFNGTRDFSAFLTIPAAIGF
                     MEENKWDQVSASCRQLVKDNAAKFFDITGGQALAPLTDDYILQLLSIPIKTSDPDKLH
                     DLLFEKYKIQVPVMQHGDKVYLRYSINAFNFQADLDKLATALKNIQSNSNLIE"
     assembly_gap    3332..3431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3590..4150)
                     /locus_tag="PLU11_06055"
     CDS             complement(3590..4150)
                     /locus_tag="PLU11_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPN58713.1"
                     /translation="MTYSDTAGYKIRNQAATHFLTFTIVGWIDIFSRKRYRDIILESL
                     DFCRKNKGLQLNAYVIMSNHIHFIWTSADKNLSGLIRDFKSHTSKRLTASIQEEPESR
                     RDWLLHLFRFHANQTNANEIFRVWTGDNHPEEIHSAHFFTTKLNYIHQNPVRNGLVAN
                     PASYLYSSAANYEGRKGLIEIDTLHW"
     gene            complement(4831..5181)
                     /locus_tag="PLU11_06060"
     CDS             complement(4831..5181)
                     /locus_tag="PLU11_06060"
                     /inference="COORDINATES: protein motif:HMM:NF039850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5763 domain-containing protein"
                     /protein_id="HPN58714.1"
                     /translation="MANIKNISLVLFFLFFSLQSGGQVFYKTPSGKKYHTDSCRMVNN
                     FSEAITIEKARQLGLQPCLICRPTNIYTGSTTATKTSGQAATVQCKGKTKAGTRCRHM
                     TRIANGYCYQHQPE"
     gene            5388..6014
                     /locus_tag="PLU11_06065"
     CDS             5388..6014
                     /locus_tag="PLU11_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HPN58715.1"
                     /translation="MKKIVLLVFLVATGLVAFSQESKTAAPEAPKWKMRFRVLGAIPP
                     ASSYDLSGDDIKISTAIAPELDFTYFFSKNLAAELILGTTHHTVKLDDGNSSTKLGKV
                     WLLPPTVNLQYHFAGKAFTPYIGAGINYSIFYGEKKEALDLGYKSNVGFSTQAGFDYQ
                     LNDKWFLNLDLKKILLKTDVTIKGAIPVVLSDVKVNPFIIGLGAGFKF"
     gene            complement(6092..6415)
                     /locus_tag="PLU11_06070"
     CDS             complement(6092..6415)
                     /locus_tag="PLU11_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58716.1"
                     /translation="MNGYKIYDIKDADRFNPYVYPEVRDELDRLFSAAYLFTTPEKEN
                     IIISYLRDHSLHSHWLLEHKELCDLVTHGKCQTSNIEALFEACRGNRQFMHSYEEYIR
                     NIDVE"
     gene            complement(6493..6903)
                     /locus_tag="PLU11_06075"
     CDS             complement(6493..6903)
                     /locus_tag="PLU11_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN58717.1"
                     /translation="MSNITELVAADKNLSTLLRGVKAADMETELSQKGPFTVFAPSES
                     AFGKLPQGELPFLLKPENKIILKTLLDSHIIPGKTAFKDFRDGQKLTTISGRELEVKV
                     LNGSVSINGATILGRDQDATNGIIHSLDIVIPAD"
     gene            7091..7537
                     /locus_tag="PLU11_06080"
     CDS             7091..7537
                     /locus_tag="PLU11_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58718.1"
                     /translation="MRYLFPFFLLVSFCSTAQGVTVMDFVQVKEGRRAEAVYFYENNW
                     KVYRDIALKKGFILSYRLEEVVADSAAGFNLVLITEYRDSVFYHNSETNFREILSTAR
                     PNGPLLLNKLQPADFRTNLFVTITRNLFRSYPASYTTENPGHLEYP"
     gene            7609..8790
                     /locus_tag="PLU11_06085"
     CDS             7609..8790
                     /locus_tag="PLU11_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPN58719.1"
                     /translation="MPDFNRRSFLRFSGLAALPFLGNASTAFSRETLSAESTLDVIKF
                     FGDGEMFEPAEYLAELQKLNAHAAIQKDRYGSGGAVEALEKKMAEITGKEKAIYMPSG
                     TMANQLAISVLSGNHTKVFVQDTSHVYRDEADAAQSVFQKRLYPLAKEETYFTAATLQ
                     TAIEQLPEQEVFGSGIGTVSIENPVRRTDGRMIPIGEIKKISEYCRSKGIKLHLDGAR
                     IYMASAWSGISIQEFSSCFDTVYISLYKYLGASGGAVLCGDKDTISKMPHLVKIHGGS
                     MYGNWTNAAMALGRLEGLEDRLLKAKAKGEELFARLNKRNDIRINPLDGGTNIYQLEL
                     TGGTDARIFQQKLNTEFSIRMPFAPGKTKSLITINETILYKDLAFILNAFSQSLTAGK
                     G"
     gene            9136..>9950
                     /locus_tag="PLU11_06090"
     CDS             9136..>9950
                     /locus_tag="PLU11_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58720.1"
                     /translation="MKTGFQLLLSMFLSFAVVSRSSAQNVAINTSGADPDESAMLDIV
                     NSSKGLLIPRVSLSSTTDAATITNPATSLLVFNTNASITGEYAAGEGYYYNAGTSGTP
                     LWRRLVGDGEVRWDDLRVVLDNGSNAASIGYLSGSSGPQIWFFRYNQSTEAMSFTVQL
                     PHAWKEGTTIYPHIHWTPKSTASGNVEWNFEYTWVNYDATTPQVFPAITSTAVVATGP
                     FTAKSHMITPLTAADAGINGTGKKISSVLVCRIWRNSDTADDTYNADAGGLFV"
BASE COUNT         2697 a         1979 c         2453 g         2721 t
ORIGIN      
        1 cgaagaaacc gatcggttcc tttattttcc tgggtacgac cggtgtgggt aaaacggaat
       61 tagcaaaagc cctggcggaa tacctttttg atgatgagag catgatgacc cggattgata
      121 tgagtgagta ccaggaaaaa cataccgtat cccggctggt gggagcgcct cccggctatg
      181 tagggtatga agaagggggc cagctgaccg aatcggtacg gaggaaacct tatagtgtgg
      241 tattgctgga tgaaattgaa aaagcacacc cggatgtatg gaatgtatta ctccaggtgc
      301 tggatgacgg acgactgaca gataacaaag gccgggtggt gaatttcaag aatacgatca
      361 tcattatgac cagcaatatc gggagtcacc tgatcatgga tgcttttgac ggaatcaagg
      421 agaaggatat tgaggaggcc aacgcaaaag caaaagtgga agtgatgaac ctgctccggc
      481 agacgatccg tccggagttt ctgaaccgcg tggatgaaat aattatgttc cagccgctgc
      541 tgaaaagaga gatcatgggg attgtaaaaa tacagctcaa ctccctgaag aaactggtgg
      601 cggagaacgg aatcacttta caattcagtg actatgccct tgagtttttg tccgaccagg
      661 gctttgatcc gcagttcggg gcccgtccgt tgaaacgcct gatacaaaag gaaattgtta
      721 atcaattatc taaacgtata ctcgccggtg atattgacaa gagcaagccg gtactggtgg
      781 atgtgtttga cggggtagtg gtattccgga atgaattgcc ggagaaagta aaaggataag
      841 gattagtata aagtattaaa gtaaaactgt aaaaggccaa ggtaaaaacc tggccttttt
      901 ttatgccaga ccggttttga tcagtaatca taatggtaac tgatacatct cattccggaa
      961 aggttcctgc ctgagttaaa ttaccggtcc tggtacaatt tattaccagt aaaacgaaaa
     1021 agttatgaca cagcaagaag agaaaacaac gatcaccagt atgccccgta ttggggaccc
     1081 tgctccgcat tttgaagcag tgaccaccca gggaaatata tctttccctg aagactataa
     1141 agggaaatgg gtcattttat tcagtcaccc ggcagacttt actccagtct gtacgtcgga
     1201 gtttatgacc tttgccaaac tggaggcgga ttttaaccgg ttaaacaccc agttggtcgg
     1261 attaagcgtg gacggattat acagtcatat cgcctggtta aggacgatca aggagcggat
     1321 tgagttccgg gatatgaaga acgtggaggt gaagtttcca ttaattgaag acatttccat
     1381 gaatgtagct acgaagtatg gcatgataca gccgaatgaa agttccacca aggcagtcag
     1441 agccgttttt tttattgatc ccaaaggaat tatccgggcc atgatttact atccgctttc
     1501 cctgggtcgg aatttcaagg aattaaagag agtgattatc ggattgcaaa ctgcggataa
     1561 acatgccgta gcgcttcctg ccgactggca gccgggtgaa gatgtcattg tacctccggc
     1621 caatagttgt ggagctgcgg ctgaccggat ggcgggcaag gatgaaggcg tttattgcaa
     1681 ggactggttc ttctgtatga agccattggc caaagaagaa gtagaaatgg cttaattcta
     1741 agttatagtg tgccgtgcat aagctgaact tatacacggc cttttttatt caatcaggtt
     1801 gctgttggat tgtatattct tcagggcggt ggctaatttg tccaggtctg cctggaagtt
     1861 gaatgcattg atggaataac ggaggtatac tttgtctcca tgttgcatga caggaacctg
     1921 tattttatat ttttcaaata ggaggtcatg cagtttgtcc gggtcactgg tttttattgg
     1981 tatactgagc aactgcaaaa tatagtcatc agttagcggg gctagagcct gtcccccggt
     2041 aatgtcaaaa aatttagctg cattatcttt caccaattgc cggcaggaag cactcacctg
     2101 atcccattta ttttcttcca tgaatccgat agccgctgga attgtcagga aggcggaaaa
     2161 atcacgggtg ccgttgaact ggtggtaatc gaggaaagtt gatgaggaag gagtggcggc
     2221 cttatatccc caactgataa ccagcggatc gatggctggt tgtttttctt tttttacata
     2281 taaaaaggaa cttcccttgg gcgtcagcat ccatttatga caggcaccgg tataataatc
     2341 cactcccaat gtagtcaggt taagcgagac ttgtccgggg gcatgtgcac catccacaaa
     2401 aacagggatg cccagggttt tggctttaat gatcacttct tccactggca gtcgcaaggc
     2461 cgtactgctg gtaatatgac tgatgaaaat cagccgggtt ttcggactga ctcctttgaa
     2521 aagttcatct acaaattctt ctttactggt cagcggtaaa gagatcggct gtcggacata
     2581 gcgggcaccg gtttgttcac agtaataatt ccaggtcttg tcacaggccc cgtactccag
     2641 gttcgtggtc agcacttcat cccctgtttt gagtttcagg cttcttgcga ctgtatttac
     2701 ggcaaaagag ggattcgtga cgtacaccaa atcgtctttg tctgcatgca ggtattggcc
     2761 caatgcctcc ctggactgtt ccaggtattt cggcccgttg cctgtaatga atagcacggg
     2821 ttcctgctcc agttccagct ggtattgctg gtatttttca aatacgggtt ttacgcaggc
     2881 gccaaaagag ccgaaattca ggaaagtgat atcatctcgt aagagaaatt gggatttgag
     2941 gtgttgcatg gggcaaatgt agtatttcgg aaaggaatag atatggctgg ggcaacataa
     3001 aaagggcagt aggcagaagg cagaaggaag taggcagaag gcagaaggca gaaggcagaa
     3061 ggcagtaggc agttggcaga aggcagtagg cagtaggcag aaggcagtag gcagtaggca
     3121 gttggcgagg cgggacggag ctaaaatgga atgtcgttga aaggcagcag gcgaggcggg
     3181 ggcagagcta aaatggaatg tcgtagaaag gcagcaggcg aggcgggaca gagataaaat
     3241 agaaggtcgt tgaaaggcag caggcgaggc gggacggagc taaaatggaa tgtcgtagaa
     3301 aggcagcagg cgaggcggga cagagataaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nggtcgttga aaggcagcag gcgaggcggg acggagctaa aatggaatgt
     3481 cgtagagagg cagcaggcga ggcgggacgc ctcgctgagc agctgcttta cagcatcttc
     3541 aagtcgccgg aggcgacata atacaaccac ggagctggaa gctccgtggt taccagtgta
     3601 gagtatctat ttcaatcagg cctttccggc cttcataatt cgccgcactg ctatagagat
     3661 aactggccgg attggcgacg agtccgttcc tgaccggatt ctgatgaata taattcagct
     3721 tggtagtaaa aaagtgagcg gaatgaattt cttccgggtg attatccccg gtccagaccc
     3781 ggaaaatttc attcgcattc gtctggttgg catgaaacct gaacagatgt aacagccagt
     3841 cacgacggct ttccggctct tcctgtatgg aagcagttaa gcgtttactg gtatgacttt
     3901 tgaagtcccg gatcaggcca ctcagatttt tatccgcgga ggtccagata aaatgaatgt
     3961 gattggacat gatcacatag gcattcaact gtagtccttt attcttccgg caaaagtcca
     4021 ggctttccaa aatgatatcc cggtaacgtt ttctgctgaa aatatcgatc cagcctacaa
     4081 tggtaaatgt caggaaatga gtggcagcct gattccgtat tttatagccg gccgtatcag
     4141 aataagtcat tttttaaatg ttgcatttga ttctgcctgc cgggctttca gcccggcagg
     4201 tgtattatct cgcctgtggc gagttgatga attacatgtt agccgctatg cgaggcgtcc
     4261 cgcctcgcat tttatcttct taatgtagct tgtgaaaagt attgttttca cattttacac
     4321 cgttatgaaa tttataagcc cgaactcatt gaacttttta ttcccaactg caaatacacg
     4381 ttaattcttt tattagcccg gtgaaaaaaa cgggtaattt ctgttttttt ctgactgact
     4441 tgtttttcta agcgaggggt tcctcctctt tgaaactatg gccggagtat agctgtaagg
     4501 cggtttctat aaccggattt ttcaataatg gttaatacag caacccgcct tccataaaaa
     4561 tcaggtccgg tcatctgatg cttttttacg ccgttgtaaa taagaattaa aaattcgtct
     4621 ggtcaggggg aatgaccgga attttaggtt tttcttctgc cggttcgtgg tagttgtctg
     4681 cacttgatca ggtactcctg acccggtata attaaagtac taagcgtcgt tttattttat
     4741 cggattgttt gcaggtaagc aggaagaggc tgtcagaaat taagggatat aatgtatgaa
     4801 aaaggttttg acagctaaag ataatccatt ctattcaggc tggtgctgat agcaataccc
     4861 attggcgatc cgggtcatat gccggcaacg ggtacctgct tttgtttttc ccttgcattg
     4921 aacggttgcg gcttgtccgc tggttttagt ggcagtcgta ctgcccgtat aaatgtttgt
     4981 cggccggcag atcaggcaag gctgtagtcc cagttgcctg gctttttcaa tcgttatagc
     5041 ttcggaaaaa ttattcacca tccggcagga atcggtatgg tattttttgc cagatggcgt
     5101 tttataaaaa acctgaccgc ccgattgaag ggaaaaaaac aagaagaaca gtactagtga
     5161 tatgtttttt atgttggcca tgctattaaa tgtacgcaag ccgggctgga attacaaaca
     5221 ggtaggcgtg tatttaaagg tggcattaaa gagcagattg tcttttaatc ataaggtaga
     5281 ataaaaaact gatttaaaat ataatattga tcatgtcctc ttgtgaaaac aatcacgaag
     5341 tggggtaatc cgtggcaact acatttgcga cctaaataga aacacttatg aaaaagattg
     5401 tactgcttgt ttttttagtg gcaaccggat tggttgcttt ttcccaggaa agtaaaacgg
     5461 ccgcacctga agcgcccaaa tggaaaatga gattccgcgt tttgggagcc attccacccg
     5521 cttccagtta tgacctttcc ggagatgata ttaaaatctc tactgcaatt gctccggagc
     5581 tggatttcac ttatttcttc agtaaaaatt tagctgccga actgatcctg ggtacaacac
     5641 atcacaccgt taaactggat gatggcaatt cttccaccaa actgggcaaa gtctggttat
     5701 taccccccac ggttaatctg cagtatcatt ttgcagggaa agcgtttacc ccttatatcg
     5761 gggcaggtat aaattattcc attttttatg gagagaaaaa agaggcgctg gatctcgggt
     5821 acaaaagtaa tgtcggattt tccacacagg ccggatttga ttatcagctg aacgataaat
     5881 ggttcctgaa cctggacctg aaaaagattc tgttgaaaac agatgtaact attaaaggag
     5941 ctattccggt tgtattatct gatgtgaaag tcaatccgtt tattatcgga ttgggtgccg
     6001 gatttaaatt ctgattgtgt cattttattt gtacaaagct gcccgttacg ggtggctttt
     6061 ttttgtggtt gaatccgtcg gtggagtaca actactccac atcaatattc cggatatact
     6121 cttcataact gtgcatgaac tgccggttac ctctgcaggc ctcaaacaat gcttcaatat
     6181 tgctggtctg gcacttgcca tgggtaacga ggtcgcatag ctctttatgt tccaggagcc
     6241 aatgactgtg aagggagtgg tcccttagat aggagatgat aatattctcc ttttcagggg
     6301 tggtgaagag ataggcggcg gaaaataacc tgtctaattc atctctgact tccggataaa
     6361 cgtaggggtt aaaccggtcg gcgtctttaa tatcgtatat tttgtacccg ttcatggtag
     6421 ggatactggt acaaaggaat ataaaaacaa ccggaattag attgattagg cacagattat
     6481 aaaaatatct ttttaatcag ccgggattac gatgtccagg gagtgtataa tcccattggt
     6541 ggcatcctgg tcacgaccaa gaatagtggc accattgatg cttaccgaac cattcaacac
     6601 tttcacttcc agttcacggc cactgatggt tgtcagtttc tgtccgtccc tgaaatcttt
     6661 gaaagcagtt tttccaggta ttatatgact atccagcagg gttttaagga ttattttgtt
     6721 ttcaggtttc agcaaaaaag gcagttcccc ttgcggcagt ttaccaaagg cactttctga
     6781 gggggcgaaa acggtaaaag gacctttttg actgagttct gtttccatat ctgcggcctt
     6841 tacgcctctt aacaaagtgg acagattttt atcagcggcc accagttctg taatgttgga
     6901 catactttgt attttatccc aggcggtatt gcctgtatac ctgtaagcta cactaattag
     6961 agtcggatag cccggttatt cccgggtttt atagttcagc agccggtttg tgcaaaactt
     7021 aatgaccggt tatataattt ttatagacac ttatggaatt tcaaactaga ttgcctgtaa
     7081 aagccagact atgcgctatt tgtttccttt ctttctgttg gtgagttttt gtagcaccgc
     7141 tcagggtgtc accgttatgg attttgtaca agtaaaggag ggccgacggg ctgaagccgt
     7201 ttacttttat gaaaacaact ggaaggtata cagggatatc gctttaaaga aaggatttat
     7261 tctttcttac aggctcgaag aagtggtggc cgattctgcc gccggcttta acctggtgct
     7321 gataacagag tatagggata gtgtttttta tcacaacagt gaaactaatt tccgggaaat
     7381 tctttcaacg gcccggccca acggaccact gctcctgaat aagttacaac cggctgattt
     7441 ccggactaac ctgtttgtta caataacccg caacctgttt cggtcctacc cggcgtccta
     7501 cacaacagaa aacccgggtc acctggagta tccgtaaatt tccaatggtg atagggaaac
     7561 tgatacaggg gcaatttgtt gtacattaga taacttaaaa gtagtatcat gcctgatttt
     7621 aaccgtcgaa gttttctgcg cttttccggc ttagctgcac tgccattttt gggaaatgcc
     7681 tcaacggctt tctcccggga aaccctgtct gcagaatcaa ctctggacgt gatcaaattt
     7741 tttggggatg gggagatgtt tgagccggcg gaatacctgg cagaattaca gaaactgaat
     7801 gcccatgcag ccatacaaaa ggatcggtac ggatcaggag gggccgtgga agcattggaa
     7861 aaaaagatgg cggagatcac gggtaaagag aaagccattt atatgcccag tggcacgatg
     7921 gccaatcagc tggctatatc tgttctgagt ggtaatcata caaaggtttt tgtacaggat
     7981 accagtcatg tttaccgcga tgaagcggat gcagcgcagt ctgtattcca aaagcgactt
     8041 tatccgctgg ccaaagagga aacatatttt acggcagcca ctttgcaaac agctattgaa
     8101 cagctcccgg aacaggaggt ttttggtagt ggtataggca ctgtttcgat tgaaaaccct
     8161 gtacgccgaa cagatggcag gatgattccc attggggaaa ttaaaaaaat cagtgagtac
     8221 tgcagaagta agggaattaa acttcatctt gatggggccc ggatttatat ggccagtgcg
     8281 tggtcaggta tttctataca ggagttttcc tcctgttttg atacggtgta tatctccctt
     8341 tataaatacc tgggtgcaag tgggggggcg gttctttgcg gggataagga tacaatcagt
     8401 aaaatgcccc acctggtgaa aattcacggg ggaagtatgt atggtaactg gacaaatgcg
     8461 gccatggccc tggggcgact cgaagggttg gaagaccgtt tgctaaaggc aaaggcaaaa
     8521 ggagaggagt tgtttgcccg gttaaataag agaaatgata tccggataaa cccgcttgac
     8581 gggggcacca atatttatca actggaatta acgggaggaa cagatgcccg gattttccag
     8641 caaaaactaa atacagaatt ttcaatacgt atgccctttg caccgggtaa aacaaaatcc
     8701 ctgattacta ttaacgaaac aattctttat aaggacctgg cctttatttt gaacgccttc
     8761 tcccagagtc tgacagcagg taaaggctga cagcccgcag cgtatttatt ttactctctg
     8821 agaaatagca gccatatata tatggtggct gggcaaatac tctgaaacac gtatgggtag
     8881 tatgggttat tacttagtta cttaaaatac taccggattt tgacaaatat catttaagtg
     8941 atcatcacat tgccataagt ttgtttacgt tgtaagtact tattacttgc agctgtttac
     9001 agtccaatcc atacttatga aaaatgtact gaccaggtac aaatcatgcc cagttgttga
     9061 ctggcagctt gttttaacta ctacttcacg cgcttttctg tttcaccagg ttattaatca
     9121 tttaaaatcc actttatgaa aacgggtttt caacttctgc tttccatgtt cctgtcattt
     9181 gctgttgtga gcagatcatc cgctcagaat gttgctatca ataccagtgg cgctgatccg
     9241 gatgaatcag ccatgctgga tatcgttaat tccagcaaag ggttattgat accaagagtg
     9301 tcgctcagct cgacgaccga cgcggctaca attacaaatc ctgcaactag tttgctggta
     9361 tttaatacca acgccagtat taccggtgaa tatgctgccg gtgaaggtta ttactataac
     9421 gcaggaacgt cgggtacgcc attatggaga agattagttg gggatggtga agttcgttgg
     9481 gatgatctgc gggtagtgct ggataacggg tcaaatgcag cttcgattgg ttatctcagt
     9541 ggtagtagtg gaccgcagat atggtttttc cggtataatc agagtacgga agcaatgtca
     9601 tttaccgttc agttgccgca tgcctggaaa gaaggtacta caatctatcc acatattcac
     9661 tggacaccca aatcaactgc atccggaaat gtggaatgga attttgagta tacctgggtg
     9721 aattatgatg caaccactcc acaggtcttc ccggctataa cctcaacagc agtagtggcc
     9781 accggaccat ttaccgccaa atcccatatg attaccccgc tcacagcggc tgatgccggt
     9841 attaatggta ccgggaaaaa aatctccagt gtgctggtgt gcaggatatg gagaaactcc
     9901 gacactgctg atgatacgta taacgccgat gccggaggac tctttgttga
//
LOCUS       DAOPMW010000085         9834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7491_length_9834_cov_8.908273, whole genome shotgun sequence.
ACCESSION   DAOPMW010000085 DAOPMW010000000
VERSION     DAOPMW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7491_length_9834_cov_8.908273"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..3152
                     /locus_tag="PLU11_06095"
     CDS             15..3152
                     /locus_tag="PLU11_06095"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58721.1"
                     /translation="MKNWILAFSLLSFSVAIAQKKKTATPVAVVNHEEQLIKGSQYRL
                     IGPFRGGRSGAVAGSYKNRTNFFFGGTGGGVWKTADAGSNWKNISDKYFGSSIGAVAV
                     APSDESILYVGEGENTMRGNVSEGLGGMWRSEDGGRTWNNIGLKDGRHIIRIVIHPRN
                     PDIVWVAVMGHLFGPNEERGIFKTTDGGKTWKKTLYVNNQTGCSDLVMEPGNPSVFYA
                     GTWRLIRTPYSLESGGEGSGLWKSTDGGETWTNISAMKGLPKNTWGIVGVAVAPSNTD
                     KIYALIENEKGGLFMSTDAGETWTLQSNDNNIRQRAWYYTKVFVDPKNENKVYCPNVE
                     FMFSVDGGKTFKSLNTPHGDHHDLWIDPEDGNRMIVADDGGAQVSFDGGNNWSTYLNQ
                     PTSQVYRLSTDNAFPYRVLGGQQDNSAFRIRSRTFGAGIMATDMETAAGSESGYVVAD
                     PTNPDITYGGNYMGMLQRLDHRTGESRMINVWPVDNMGAGAVAAKYRFQWNYPIFFSP
                     HNPKRLYAAGNHLFVTENEGRSWEQLSPDLTTNDKTKQASSGGPVTQDNTSVEYYCTI
                     FTAAESPLEKDLLWTGSDDGLIHVSKDGGKNWENVTPKDCPPMMMWNCIDADPFKKGT
                     AYFAGTRYKSDDFRPYLYMTEDYGKTWKAISNGIPALHFTRAIRADKKRPGLLYAGTE
                     FGMYISYDGGANWRSFQHNLPVTPITDLLIKNNDLIVATQGRSLWIMDDLSVVQQAKE
                     EILNKSLHVFDVNPAYRTPGGGRGRRGGGGDIQNAGMNPASGVVINYYLKNITDSTKL
                     SVALLDKSNKLIKTYSTTSKENKIDLKKGMNQFIWDMNHPEADRMEGLILWNGFVRGP
                     KAAPGEYTARFRSGTDSADVKFTILGDPNYSTTNAEYEEQVNFLLAIRDKFTAVMKGM
                     KNIKDIRQQLSDISGRVGKAIPRELKQQMDTINKQLTAIEEALHQTKAKSGQDVLNYP
                     IRLDDKIAGVYNAAAAGNTAPSKQAKEAFADLAAQTDIQLQQLKYVLDEELKKLNLLI
                     HEVRLPVVAPKREVD"
     gene            complement(3465..4502)
                     /locus_tag="PLU11_06100"
     CDS             complement(3465..4502)
                     /locus_tag="PLU11_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HPN58722.1"
                     /translation="MKALTVITDPVYKEPAGFTAYEKFWLKYIQDKRDLPFIHLLTSI
                     HILVIPVAILLYTPVLEGWYWWLAYIPYFYVSQMYFKGRFGLMLHCICHRKLFKKNYT
                     WLFHYIIWIVCPFFGHTPETYFAHHMGMHHVENNMEDDASSTLRYQRDSLSGFLAYVG
                     RFLVLGFSDTFMYFFSRKRKKFYMRLTAGEMSFYLFCIAMCFVNLKATLFIFIIPFVF
                     ARVVMMLGNWTQHAFVNLEDLEDNTINCINTKYNQMCWNDGYHAIHHIRPAMHYTEIP
                     GEFLKTKDELARKKILVFDGIHYLHIFIWLMTKRYDKLAHNLVNVNNMFSSEEEAIRV
                     MRERTKKVKSS"
     gene            complement(4629..6077)
                     /locus_tag="PLU11_06105"
     CDS             complement(4629..6077)
                     /locus_tag="PLU11_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN58723.1"
                     /translation="MSNSRRNFLQKIAIGTGALAAGIPSLARVTGASDEQMERSEGWE
                     KKGQRFNMCGYAAPKLDVVRIGVVGLGMRGSDAVERLSYIDGVEITALCDKYSDRVTA
                     AQKTLEKTGRPKAKEYSGEEGYKALCESNEVDLVYTPTPWHLHTPVALYAMRHGKHAA
                     TEVPAGKTMDELWDLVETSEKTKQHCMMLENCCYDFFELLTLNMARQGMLGELVHAEG
                     AYIHDLSKDWNFNKKAYADMWRLKENIGHNGNLYPTHGLGPIAQCMDINRGDRFDHLV
                     SMSTNDFTMNNMAKELAAKDDFFKEYVDRPYRGNMNTTLIRTFKGKTIMVQHDVSTLR
                     PYSRIHLVSGTKGAAQKWPGPQRIAMGHSWMKPEDQKKLEEKYALPIVKHIGEIAKEV
                     GGHGGMDFIMDWRLIDCLRNGLPLDQDVYDGAAWSCLMPLSERSVNKKSRTIDIPDFT
                     RGAWQTNKPVDLTLNGGGTTGVRKQKPSLQMK"
     gene            complement(6162..7697)
                     /locus_tag="PLU11_06110"
     CDS             complement(6162..7697)
                     /locus_tag="PLU11_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPN58724.1"
                     /translation="MKKRFLFWMTPVFFAGFACTAPKNSSSQTTTTSTPPAFTAQREF
                     RAAWVATVANINWPSKPGLSTEEQQREAIALLDFLEVHHFNAVILQVRPQCDALYESE
                     LEPWSYYLTGKQGQPPSPFYDPLKFWTDAAHERGIELHVWLNPYRAHHKDGKEITEKS
                     IVKKRPELALYLKEGYWWLDPAQKGTQEHSTAVVMDLVKRYDIDGVHFDDYFYPYPSY
                     NNGEDFPDSVSWKAYQQSGGKMSRGDWRRDAVNVFIEQLYKKIKAAKPWVKFGLSPFG
                     IWRPGYPESVGGFDQYEQLYADAKLWLNKGWIDYFTPQLYWPINRPAQSFPVLLGWWA
                     GENTMKRHLWPGISVGRDTGFRNVNEIMSQIQISRGMLPESKGVVHWSISSVTKNPAM
                     AKALLETPYKKQALVPASPWLDNTPPESPNMQLMVKDGMAHISWTHPDIKDVFRWVLY
                     YEYGEGKVKEYTILNQKTDHIELSTEKIMDKNKLAMTFVRLTAVDRCGNESRQPVIAE
                     VIR"
     gene            complement(7751..8431)
                     /gene="trmD"
                     /locus_tag="PLU11_06115"
     CDS             complement(7751..8431)
                     /gene="trmD"
                     /locus_tag="PLU11_06115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPN58725.1"
                     /translation="MHIDILTVLPELLESPFSHSMMKRAQEKGHLTIRVHQLRKWAVN
                     DYGQVDDYQYGGGAGMVMMCEPLAKAIEELSADRKFDEIIYMTPDGVTFNQKMANAFS
                     LKENLLIICGHYKGIDERVRQQFVTKEISIGDFVLSGGEIPAALLVDAIGRLIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPVDFRGLKVPDVLMSGNHKLIEDWRHDQAIQRTKDR
                     RPDLLQGQ"
     gene            complement(8452..8829)
                     /locus_tag="PLU11_06120"
     CDS             complement(8452..8829)
                     /locus_tag="PLU11_06120"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPN58726.1"
                     /translation="MNPLRFSLFLLTLFSACGKPAAEKDKIFPVVSISSPANGQNFTP
                     GQPIPITALVTDETYIAEVHIHVSNTNTGDLLMDVHEYPAGKTGNISQSITAVAGVNY
                     KIQVIAKDRAVNETNSTVLVSCN"
     gene            9238..>9834
                     /locus_tag="PLU11_06125"
     CDS             9238..>9834
                     /locus_tag="PLU11_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HPN58727.1"
                     /translation="MKLTSSTVINENACAADIVKEHYRTASVFRKYAIEYCCGARWPL
                     KMVLETQQLNTEQVLAELEAACRQVTIPASLPYDTWSMDFLTEYIINIHHAYLRKSLP
                     VIKDQVTRFVEEHRKKYPYLSEVERKVSFLDKTMIPHLLQEEEVIFPYIRQISHAYES
                     KESYAKLLVRTLRKPVEDLMHDEHDTMEKTLTQLRELTN"
BASE COUNT         2567 a         2251 c         2414 g         2602 t
ORIGIN      
        1 ttcaatcact gtttatgaaa aattggatat tggccttttc ccttctatct ttttccgtgg
       61 cgatcgccca gaagaaaaaa acagctaccc cagtagcggt agtcaatcat gaagaacaac
      121 tgatcaaagg cagccaatac cggctgatcg gtcccttccg cggtgggcga agcggcgcgg
      181 tggcgggtag ttataaaaac agaacaaatt tcttctttgg aggaactggt ggtggtgtct
      241 ggaaaacggc ggatgcaggg agcaactgga aaaacatttc cgacaaatac tttggcagct
      301 ccattggtgc ggtagcggta gcgccttccg atgaatcgat cctctatgtg ggtgaaggtg
      361 agaataccat gcgcggaaat gtaagcgaag gacttggcgg catgtggcgc agtgaggatg
      421 gcgggcgtac ctggaataat atcggcctca aagacgggcg tcatattatc cgcatcgtta
      481 tacatccacg taacccggat attgtgtggg tggcagtgat gggacacctg ttcggaccga
      541 atgaagagcg cggcattttc aaaaccaccg atggaggtaa gacctggaaa aaaaccttat
      601 acgtcaataa ccagaccggc tgcagcgacc tggtcatgga acccggcaat ccttccgtat
      661 tttatgcggg tacctggcga ctgatccgca ctccctattc attagagagt ggtggtgagg
      721 gcagcggtct atggaaaagt accgatggcg gtgaaacctg gaccaatata tccgcaatga
      781 aaggattacc taaaaacacc tggggcattg taggggtggc cgtagcaccc agtaataccg
      841 ataaaattta tgcattgata gaaaatgaaa agggcggtct cttcatgagt accgatgccg
      901 gggaaacctg gacccttcaa tccaatgata ataatatccg tcagcgagcc tggtattata
      961 ccaaggtatt tgtagatccg aagaatgaaa acaaagtcta ctgtcccaat gtggagttta
     1021 tgttcagtgt ggatggcggc aagacattta aaagtttaaa tacaccccat ggtgatcatc
     1081 atgatctctg gatcgatccc gaagatggga accggatgat tgtggccgat gatggcggtg
     1141 cacaggtatc gtttgatggc ggcaataact ggagtacgta tctgaatcag cctacttccc
     1201 aggtataccg gttgagcacg gataatgcat ttccgtatcg cgtattaggc ggacaacagg
     1261 acaacagtgc tttccgtatc cgtagccgta cgtttggggc agggattatg gctaccgata
     1321 tggagacagc agccggtagt gaaagtggtt atgtagtggc tgatccgacg aacccggata
     1381 ttacttatgg aggaaactat atgggcatgt tgcaacggct tgatcaccgg accggcgaga
     1441 gtcgcatgat caatgtatgg cccgtggaca atatgggcgc cggtgcggtg gccgcgaagt
     1501 accgtttcca atggaactac ccgatcttct tctctccgca taatccgaag cgattgtatg
     1561 cagcagggaa tcatttattt gttacagaga atgaaggacg cagctgggaa cagttgtctc
     1621 ccgatctcac taccaatgac aaaaccaaac aggcgtccag tggcggacct gtaacacagg
     1681 acaatacttc agtggaatat tattgtacga ttttcacagc ggctgagtcg ccattagaga
     1741 aggatctttt atggaccggc agtgatgatg ggctgattca tgtaagtaag gatggaggta
     1801 aaaactggga gaatgtcacc ccgaaagact gcccgccgat gatgatgtgg aattgcattg
     1861 atgccgatcc gtttaaaaag ggtaccgcat actttgccgg tacgcgctat aaatcggatg
     1921 atttcagacc ttatctgtat atgacggaag attatggcaa aacatggaag gctatcagca
     1981 atggcatccc tgctttacat tttacccgtg cgatacgagc cgataaaaaa cgtccgggtt
     2041 tgttatatgc cggaacagaa ttcggcatgt atatttccta tgatggcggc gccaactggc
     2101 gttctttcca gcataacctg ccggttacac ccatcacgga cctgctgatc aaaaataatg
     2161 acctgatcgt ggctacccag gggcggagtc tctggattat ggacgacctc tcggtggtgc
     2221 aacaggccaa ggaagaaatt ttgaataaaa gcctgcatgt atttgacgtt aatccggcct
     2281 atcgaacacc cggtggtggt cgtggtcgcc ggggaggcgg aggcgatatt caaaatgcag
     2341 gaatgaatcc ggcttccggc gtcgtgatca attattatct caaaaatata acggattcca
     2401 ctaagctttc tgtggccctg ctggataaaa gtaataagct gattaagacc tattccacca
     2461 cctcaaaaga gaataagatc gacttgaaaa aaggaatgaa ccagtttata tgggatatga
     2521 atcaccccga agcagatcgc atggaaggcc tgattctgtg gaatgggttt gtacgtggtc
     2581 ccaaagcagc acccggtgaa tataccgccc gcttccgttc cggtaccgat tcagccgatg
     2641 tgaagtttac cattctcggt gatcctaatt attccaccac caatgcagag tatgaagaac
     2701 aggtgaattt tctgttagcc attcgtgata aattcacagc ggtaatgaaa ggaatgaaga
     2761 atatcaaaga tatccgtcag caactgtccg atatcagtgg ccgtgttggc aaagccattc
     2821 cccgcgaatt gaaacaacag atggatacga tcaacaagca actgaccgct attgaggaag
     2881 ccctgcatca gacgaaagcg aaaagcggac aggatgtact gaattacccg atccgtctgg
     2941 atgacaaaat cgccggtgta tacaatgctg ccgctgccgg taataccgct ccttcaaaac
     3001 aggccaaaga agcttttgct gacctggccg cacaaaccga tatacaactc cagcaactga
     3061 agtatgtgct agatgaggaa ctgaaaaaac tgaacctgct gattcatgaa gtcagactgc
     3121 cggtggtggc gccgaagaga gaagtagact agttcgtagt tcgtagtcgg tagttcgtag
     3181 tctcatttca gaagaccttg gattttagtt gaaagaggtg aaaagaaaga ggcaggcttc
     3241 agtcggcagg aggcagtcag cagtcaaagg cgcttgttaa aaaatattag ttcaaacatt
     3301 tcagtcatac tttgtacttc gtatctcgta ctaaaaagga gaaatggcat catctgctat
     3361 ttctcctttt tagtttattt cattgaattt ttgtctgtat ctgcttttta ttcttatctg
     3421 acggttatcc acgttccaga aaaagcaaga ctactatcta ctaactacga acttttaact
     3481 ttcttagtgc gctctctcat caccctgatc gcttcttcct ccgaactgaa catattgttt
     3541 acgttcacca ggttatgggc cagtttgtcg tatcgtttag tcataagcca gatgaagatg
     3601 tggaggtaat ggatgccgtc gaagaccagg atttttttac gggccagttc gtcttttgtc
     3661 tttaagaatt caccggggat ttcagtataa tgcatggccg gacgtatatg gtgaatggca
     3721 tggtagccat cgttccagca catctggttg tattttgtgt tgatacagtt gatggtatta
     3781 tcctccaggt cttccaggtt cacaaaggca tgctgggtcc agttgccaag catcataacc
     3841 acccgggcaa atacaaaggg aatgatgaag atgaagagcg ttgctttcag gttcacaaag
     3901 cacatagcaa tacaaaagag gtagaacgac atttcaccgg cggtcagccg catgtaaaat
     3961 tttttgcgct tgcggctgaa gaaatacata aatgtgtcgg aaaacccgag taccaggaaa
     4021 cggcccacat atgccaggaa gccagacagg ctgtcccgct gatagcgcaa cgtactgctg
     4081 gcatcatctt ccatattgtt ttccacgtga tgcatgccca tgtgatgggc gaagtaggtt
     4141 tcaggggtat ggccaaaaaa cgggcagacg atccagatga tatagtgaaa caaccaggtg
     4201 taatttttct taaacagttt gcgatgacaa atgcaatgca gcatcaaccc gaaacggcct
     4261 ttaaaataca tctgggaaac atagaaatag ggtatataag ccagccacca gtaccagcct
     4321 tccagcacgg gggtatataa taaaatggca accggtatca ccaggatatg aatcgaggta
     4381 agcaggtgaa taaagggcag gtctctttta tcttggatgt actttagcca gaatttttcg
     4441 taagccgtaa aaccggcggg ttctttgtag acggggtctg ttattactgt cagtgccttc
     4501 atgaaattat tagttcagtc aataagcagt taatacacgt acgcaactat aaaattacag
     4561 gtttaaaagc aatatgcctg ctttgtttaa caaaacaggc atagcagaaa attcagttcg
     4621 gattgaaact atttcatttg caaggatggc ttttgtttcc gtacaccggt tgtgccacca
     4681 ccattgaggg tgaggtcaac gggtttattg gtctgccagg caccccgggt aaaatcgggt
     4741 atatcgatcg tccttgattt tttattcact gatcgctcac tcagcggcat caggcaactc
     4801 caggcggctc catcatatac atcctgatcc aacggtaatc cattacgcag gcaatcgatc
     4861 agccgccagt ccataataaa gtccatgccg ccatgtccgc ctacctcttt cgcaatctcc
     4921 ccgatatgtt taacaatcgg cagggcatat ttttcttcca gctttttctg atcctccggt
     4981 ttcatccagc tatgtcccat cgcgatgcgt tgtggtccgg gccatttttg ggcagctcct
     5041 tttgtaccac tcaccaggtg gatccgggaa tagggccgga gtgtagatac atcatgttgt
     5101 accatgatcg tcttgccctt aaacgtgcgg atgagtgttg tattcatatt gccacggtag
     5161 ggccgatcca catactcttt gaagaaatca tctttggccg ccagttcctt ggccatatta
     5221 ttcatcgtaa aatcattggt actcatactg accaggtgat cgaagcggtc gccccggttg
     5281 atatccatac attgggctat cggtcccaaa ccatgggtgg gataaagatt tccgttgtgg
     5341 ccgatatttt ctttcaaccg ccacatatcg gcataggcct ttttattaaa gttccagtcc
     5401 ttactcaggt catggatgta ggcgccttcc gcatgtacga gttcaccgag cattccctgg
     5461 cgggccatat tcagggtgag cagttcaaag aaatcgtaac aacagttttc cagcatcata
     5521 caatgctgct ttgttttttc tgaggtttcc acgaggtccc ataattcatc catggtttta
     5581 ccggcaggaa cttcagtagc ggcatgttta ccatggcgca tggcatataa ggcaaccggt
     5641 gtatgcagat gccagggggt gggcgtgtaa acaaggtcca cctcattgct ttcgcaaagg
     5701 gctttgtagc cttcttcccc actgtattct tttgctttcg gacggcccgt tttttccaat
     5761 gtcttctggg cggccgtcac ccggtcagaa tatttatcac agagcgcggt gatttccacg
     5821 ccatcgatat agcttaatct ttctaccgca tcagaacccc gcatccccag tccgaccacg
     5881 ccaatacgta ccacatccag ttttggcgct gcgtaaccgc acatattaaa acgttgtcct
     5941 tttttctccc atccttcaga acgttccatt tgttcatccg atgcgccggt tacccgggcc
     6001 agggaaggaa tgccggctgc cagggcaccg gtaccaatcg ctattttctg taaaaaattt
     6061 ctgcggctgt tactcatagc aaacaatttt taggttcctg caatttcatg attttatcgg
     6121 gtcaatatgg atttgccggt aaaatggcac aatcgtttga ctcatcggat cacttcggca
     6181 atcacgggtt gccggctttc attgccacaa cggtccacgg cggtcaggcg tacaaaagtc
     6241 atggccagct tgttcttgtc catgattttt tcggtggaca gttcaatatg atccgttttc
     6301 tgattcagta ttgtatattc tttcaccttc ccttccccgt attcataata aagtacccag
     6361 cgaaacacat ccttgatatc cgggtgagtc cagctgatat gtgccatccc gtcttttacc
     6421 atgagttgca tattgggtga ttccggaggc gtgttatcca gccatggact ggccggtacc
     6481 agggcttgtt ttttataagg tgtttccagc agggctttcg ccatggccgg atttttagta
     6541 acggatgaaa tactccaatg taccacgccc ttactttcgg gcagcatacc ccggctgatc
     6601 tgtatctggc tcatgatttc attgacattt ctgaaaccgg tatcacgacc cacactgatg
     6661 cccggccata aatgtcgttt catcgtgttt tcaccagccc accatcccag taaaacagga
     6721 aaactctggg ccggccggtt gatcggccag tagagttgtg gagtgaaata atcgatccag
     6781 cctttattga gccaaagttt ggcatctgca taaagctgct cgtactgatc aaaaccacca
     6841 actgattccg gataacccgg acgccagata ccaaagggac tgagaccgaa tttcacccag
     6901 ggtttggcag cttttatttt cttatagagt tgttcaataa atacattcac ggcatcacga
     6961 cgccagtcac ctcttgacat tttgccgccg ctttgctgat acgctttcca actcaccgaa
     7021 tccggaaagt cttcgccatt gttataagaa ggatagggat aaaaatagtc atcgaaatgc
     7081 accccatcaa tgtcatatcg ttttacgagg tccatcacta cggcagtgga atgttcctgc
     7141 gtgcctttct gcgccgggtc cagccaccaa tatccttcct ttaaataaag ggccagttcc
     7201 ggcctttttt tcacgatgga tttttcagtg atctctttgc cgtctttatg atgggcccgg
     7261 tagggattca gccagacatg cagctcgatg cctctttcat gggcggcatc ggtccagaat
     7321 ttcagcggat cgtaaaaagg ggaaggaggt tgtccctgtt tgcctgtcag gtagtaagac
     7381 cagggttcca gttcgctttc gtagagggca tcacattgcg gacgcacctg cagaatgacg
     7441 gcgttgaaat gatggacctc gagaaaatcc aacagggcga ttgcttcacg ttgttgttct
     7501 tcggtgctca aaccgggttt actgggccag ttgatattgg ctactgtggc cacccaggcg
     7561 gccctgaatt cgcgctgggc ggtaaaggcc ggtggcgtgc ttgttgttgt tgtttggctg
     7621 ctgctgtttt ttggggcggt gcaggcgaac cctgcgaaaa atacaggggt catccagaaa
     7681 aggaatcgct ttttcatgtg caaactttta gtgtggaagc aataggttac taaaagtacg
     7741 ctaattcagt ctactgcccc tgtaacaggt cgggccggcg gtctttggtt cgctggatgg
     7801 cctgatcgtg cctccaatcc tcaatgagct tatggttgcc gctcataagt acatcgggta
     7861 ctttgagtcc gcgaaaatca accggacggg tgtataccgg cggggccagt aaattatcct
     7921 gaaaagaatc agtcagggcc gacgtctcat cattcagtac accgggtatc agccggccaa
     7981 tggcatccac gagcagggcc gcgggaattt caccgccgct gagcacgaaa tcgccaatgg
     8041 agatttcttt ggtaacaaat tgttgtctta cccgctcatc aatgccttta tagtgtccac
     8101 agatgatcag cagattttct ttgagggaaa aagcgttggc cattttctgg ttaaaagtga
     8161 cgccatcggg cgtcatataa atgatctcat caaatttccg gtcggccgaa agttcttcga
     8221 tcgctttggc gaggggttcg cacatcatca ccataccggc gccaccacca tattggtaat
     8281 catccacctg tccatagtca ttcaccgccc atttgcgtag ctggtgtaca cggatggtca
     8341 ggtggccttt ttcctgcgct cttttcatca tactatggga aaaggggctt tccagtaatt
     8401 cgggtaaaac ggtcagaata tcaatatgca tgcggcaaag ataggaactg ctcagttgca
     8461 ggaaaccaat accgtactgt tggtttcatt gaccgcccgg tccttggcaa tgacctggat
     8521 cttataattc accccggcca cagcagttat ggactgactg atattgccag tcttaccggc
     8581 cgggtattca tgtacatcca tcagcagatc accggtatta gtattgctga catgtatatg
     8641 tacttcagcg atataggtct cgtctgtaac cagggcggta atgggaatcg gttgtccggg
     8701 cgtaaagttt tgtccattgg ccggactgct gatcgataca acaggaaata ttttgtcctt
     8761 ttccgcggca ggtttgccgc aggcggagaa cagggtcagt aagaatagag aaaaccggag
     8821 tggattcata caatgaagtt aagaaaatag tgaatgggga gtggggaatg gtgaatggtg
     8881 agtagtgagt agtgagtggg agggagtatc tgctttagga gacttcgtac ttcgtacctc
     8941 gtacttataa aagagaaacc cccagggaac tgggggttga cattgaaaca aaaaccaacg
     9001 atagctcatt ttcatgagcc acattttaag tgtatttttt cacctctggg tcaaaagtac
     9061 aacggcctct agtacctgaa agtgaccttc atcatgccag taagtgatat aagtcagtct
     9121 ctttttttac gggttttttc ccagttggcc gctgctggtc ttttttgaat ataaaagatg
     9181 atttaaatca ggtttttggg atatccagaa tgttattttg cccgtgaaac ttgtaacatg
     9241 aagctgacct cctctacggt gattaatgag aatgcctgtg ccgcggatat tgtgaaagaa
     9301 cattaccgta cggcttctgt tttccgtaag tatgctattg agtattgttg cggggcccga
     9361 tggccgctga aaatggtgct ggaaacccaa cagctgaata cggaacaggt attggcagaa
     9421 ctggaagccg cctgccgcca ggtcactatt ccggcatctt tgccatatga tacctggagc
     9481 atggattttc tgacggagta tattatcaat atccaccatg cttatttacg gaaatcactg
     9541 ccggtaataa aagaccaggt aacccgcttt gtggaggaac accgcaaaaa gtatccctac
     9601 ctgtccgaag tggaaagaaa ggtcagtttt ctggataaga ccatgatccc gcatctgctg
     9661 caggaagagg aagtgatttt tccttatatc cgccagatca gtcatgcgta tgaaagtaaa
     9721 gaatcgtacg ccaaattatt ggtgcgtacc ctgcggaaac ccgtggaaga cctgatgcat
     9781 gacgaacatg acaccatgga gaaaacgctg acccaactga gagaattaac caat
//
LOCUS       DAOPMW010000086         9810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7523_length_9810_cov_10.685700, whole genome shotgun sequence.
ACCESSION   DAOPMW010000086 DAOPMW010000000
VERSION     DAOPMW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_9810_cov_10.685700"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PLU11_06130"
     CDS             <1..1683
                     /locus_tag="PLU11_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPN58728.1"
                     /translation="FFMSHSVERYVIKHLSGSKINQVEEFDFHKKELSIGRAASSDIQ
                     FDPEREVVVSREHGLIVKSGNDPLRFSITDNNSRNGIFVNKNRVKGTVDIQPGDEIQL
                     GNNGPVFTFDIYPRPQDMMAATRLVEIPTSIKATSISTITEPPVVLTAEPVKTGLGKQ
                     TVERMLVAERKKSYSTMVFIIGGLVLALGGLGFAFRDKLFKKSTTVINNIVADSANKN
                     KMSPEVISSQNDNKVVQIEFGWQLKDTRTGEELWHEFVPVKAQNGATQYMALYIRNNQ
                     GVIEPYINVRRNTGEGIPVGMTGASGSGFVVSEDGYILTNRHVGAGWHTRWQFEQFAF
                     PGLLVSVNESGKLAVDYNSRVMQNDVFGWVPGNATMVGGQPVMPGVIQGENTYLNVVF
                     SGTSERRMARLAQPSDEHDVALIKVDIPTPLQKVKMKDAEGTVQPGQSVTVMGYPGIA
                     PTQVSVRNSKDPFNPGMKEATVPTPTVTPGNIGRIIPATSMKDMRYSGFGDSYQLTIN
                     ATGSGNSGGPLFDGEGNVIGIYYAGATDAQGTRITFAIPIKYGLQLMGIRKS"
     gene            1764..2951
                     /locus_tag="PLU11_06135"
     CDS             1764..2951
                     /locus_tag="PLU11_06135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN58729.1"
                     /translation="MFIPAHTEIAGYQLGDKIGTGGMGEVYSAWHPVLRRKAAVKILH
                     KIEHTERFRQEAYIQSSVDHPHIARLFEFTYTGKTPCIIMEYVEGEPLDSYLSRQGKL
                     ESQELIQLLTQISKALAYLHEREIFHRDIKPSNFKRLPDGSVKMLDFGIAKHKYSPRL
                     TQQGFVVGTTEYMAPEQFEQQADLRSDIWSLGVMTYELATGYLPFEATNPVTLRARIS
                     KGSFSNPRLFVPELSELLRQVIEQCLRINPAQRPTAVQLQALLTGKELSKNKEQKKWP
                     VKSPVLISVISGLAIILLVWLLNTPDVPKQPDPPVKPVSGSEIKPAERSITINVPGVE
                     HAELVFPDGSRQTAPFSITGKDGELVRFRIEAAGYREKEVSFDLRMGAYTYEFVLDKI
                     NNE"
     gene            2951..3850
                     /locus_tag="PLU11_06140"
     CDS             2951..3850
                     /locus_tag="PLU11_06140"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPN58730.1"
                     /translation="MFWKKLFTKNKQSPLTEKMPATGDTPNLQVVVRSDLGNIRTNNE
                     DTGLFYRIADEKVIREKGCLLMVADGMGGHQAGEVASRMASEIIIREYFSPQKNGGIE
                     KNLRRAFIVANTSIHQAAASNKEQQGMGTTCTALVVTDPVIHFAHVGDSRAYLLKKGG
                     LSRITEDHTYVQELVRSGEITAAEADTHPQRNILTNAMGTKADMKVDAGRYALLFETG
                     DRLMLCSDGLYDYLNDDEIAAFLGGHDLTEAAEQMIVTAKKRGGHDNITVVLAQKKSG
                     KEEVPARETRDFDLPVTKELDLP"
     gene            3847..5607
                     /locus_tag="PLU11_06145"
     CDS             3847..5607
                     /locus_tag="PLU11_06145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN58731.1"
                     /translation="MIGQSIQNYQVTAHLGEGGMGTVYKATDTVLGREVALKMLHTPL
                     INQAQFLERFRKEARILAQLLHPNIAVIYNFIEQNNSHYMVMEYVEGKNLDSLLRQQR
                     VLSYQVVVPVFIQALEGLHHAHKKGIFHRDIKPSNLILTPDGTVKLMDFGIAKMAGEQ
                     RMTQVNRVVGTVEFMAPELIQGKDPSVASDIYATAVTMYELLTGKLPFESTTDFNLMQ
                     EIMQKKPPAPDKLNTAIPKQLSSILMKAMEKKPENRFADARALQLALLSSFPELKEFS
                     LDRVSDTVPATQFISTNKAQPLATQVEMNRTGDQTIPAAFTSRIKDWYSRTNKKHLAI
                     ALVLALMLVSMAVILLTGNESPAAVTDQPSGPAPTTNSTQLVTENRNSQTDQLIPVNP
                     GPPADNNTPVTPPSDKVEPINPAGNDKEKKKETPKPKDTEQGKVKTPVTPKEEKPAPP
                     PEVTTPKEILKPIIPSPEETSKRAPRTVSLMTKTEVSLYLQQSINASTAFEGQGLSFT
                     VTRPVYYNGEMIIEKGATATGRITKAGNKKLGLVISTVTAANGQRIPLQETELSGRIE
                     EILSSRNYSAFLKKGITITL"
     gene            5625..6248
                     /locus_tag="PLU11_06150"
     CDS             5625..6248
                     /locus_tag="PLU11_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58732.1"
                     /translation="MKKITCMLFLLVSLAADAQIKQRSTTKGFSAAVQVHTLGWTSEY
                     FKYLDEGAPNGFGGGIRLGYGINEMIEPYFAYDFTKINLGDIDAQSYKMNHIDLGVRL
                     NFVGNVHPVRPFVEAGYTYLKGTIKQVIAGSSYVDAEFYGGKPHLGGGVSFFPSIPIN
                     LFAKGIFTVGKKSNLKQDGIVDTDKPDVTTFRITVGIGFNIQELMNK"
     gene            complement(6305..7108)
                     /locus_tag="PLU11_06155"
     CDS             complement(6305..7108)
                     /locus_tag="PLU11_06155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58733.1"
                     /translation="MEVYNDYFEANRQLWNQRTMAHRDSSFYNREGFLKGERVLTPIE
                     LGELEDLKGKTLLHLQCHFGMDTLDLARMGAEVTGVDLSDEAIRQAKQLSDEIGTRAR
                     FICCNVYDLEAHLDETFDRVFTSYGTIGWLPDLDKWAGLIARYLKPGGQFYMAEFHPV
                     LWMFDDDFTRIQYSYENREVIITENQGTYTDRNADIKGKEYSWNHSISEVLNALIRAG
                     LKLELFNEHMFSPYSCFRNMTETTTGKWEIKGMEGKLPMVYSLKAVKAG"
     gene            7297..8637
                     /locus_tag="PLU11_06160"
     CDS             7297..8637
                     /locus_tag="PLU11_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN58734.1"
                     /translation="MKYTRRQQLNRMAGLITGLFLFATAALAQQPVVFLTLKEATDST
                     LANNRDLGLSRLDEKIAKAKTKEMDAIYLPQADFSFTALSSNNPLNAFGFKLQQQAVQ
                     QKDFNPDLLNKPGASGDYSGRLQVKQPLLNMDLVYMRKAVAAQEGIYQMKTQRTTEYL
                     AYEVKKGYLQLQLLYEAEKVLKEALRSANAMYNFTDNRVKEGLMQKSDALNVKVWIHT
                     VETNLAETTSNIGNASDYLSLLMGRPYGTVYTAEPVQTENTDLSGASVPANRADFAAM
                     KKAMEASSLMIKSAEKSYLPRINAFASYQVNDYRLTGFGAGSYLAGLQLSWDIFKGNS
                     TKNKIITQQTELSKLSGQLENQQVQSQLELNKTLRQLEDARFKIRQQTAAIDNATEAL
                     RILQDRYEQGLVNSTDVLQAQTQLSQQQLGKAQAVFSYNSTAAYISFLTSTQKN"
     gene            8648..9748
                     /locus_tag="PLU11_06165"
     CDS             8648..9748
                     /locus_tag="PLU11_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN58735.1"
                     /translation="MSYNKFIPGVLLLSGILLISSCKGKKETPVSKDAAPVTVYTATP
                     GGTVEGGIAVSGKVEALQTAQLSTKLMGTITRIFVKTGDRVRRGQTLATISSQDITAK
                     RAQTSAAILEAEANVKNAQKDFERFTNLYNKQSASAKELDNVTLQYNAAKARLEAASQ
                     MKNEVNAIASYSTITAPFDGVVTQRMADEGNMANPGMPLLVVEQNTQLQISATVSEDQ
                     VGLLKKGSKAQVEIKAAGKSIECVVNEISPSSQMTGGQFEIKLSIPESQKKDLYAGMY
                     VNVFIPVSGAAVSSQPSGIVLVPTSSLVQNDQLTSIYTISSNNTALLRWVRTGKVYGD
                     QTEILSGLAATETYITKAEGKLYNGVPVTVKK"
BASE COUNT         2866 a         2482 c         2315 g         2147 t
ORIGIN      
        1 ttttttatga gccattcagt agaacgttat gttattaaac acctctccgg cagcaaaatc
       61 aaccaggtgg aggaatttga tttccacaaa aaagaactca gtatcggccg ggccgccagc
      121 agcgacatcc agtttgatcc tgaacgggaa gtagtggtaa gccgtgaaca cggccttatc
      181 gtcaaatccg gcaacgatcc gctccggttt tctataacag acaacaatag ccgaaacgga
      241 atttttgtca ataaaaacag ggtgaaagga acagtggata ttcaaccagg tgatgaaata
      301 caactgggta ataacggacc ggtattcacc ttcgatattt atccccgtcc ccaggatatg
      361 atggcggcca cccgcctggt agaaattcct accagtatta aggctaccag catctccacc
      421 atcaccgagc ctccggtggt gctcaccgct gaaccggtga agaccggact gggcaagcaa
      481 accgtggaac gcatgctggt ggcggaaaga aaaaaatcct acagcacgat ggtctttatc
      541 attggcggcc tggtgctcgc cctgggcgga ctgggctttg ccttccgcga caagctgttc
      601 aaaaaaagta caacggtcat caacaatatt gttgctgatt ctgccaacaa aaacaagatg
      661 agccccgaag tcatctcaag tcagaatgat aacaaagtgg tacaaatcga atttggctgg
      721 caattaaaag atacccgtac cggtgaagaa ctctggcatg agtttgtgcc ggtaaaagcc
      781 caaaatggcg ccacacagta tatggcgctg tatatccgga acaatcaggg tgtcatagaa
      841 ccatacatta atgtcagaag aaataccggc gaaggtattc cggttggtat gaccggtgcc
      901 agcggatccg ggtttgttgt atcggaagac ggctatattc tgacgaaccg gcacgttggc
      961 gccggctggc atacgcgctg gcagtttgaa caatttgcat ttccgggtct gctcgtaagt
     1021 gtaaatgaat ccggaaaatt agccgtcgat tataattcca gggtgatgca aaacgacgta
     1081 tttggctggg taccgggtaa tgccaccatg gtaggcggtc agccggtgat gcccggtgtg
     1141 atccagggag aaaataccta cctgaatgtg gtgttctccg gaacctctga acgccggatg
     1201 gccagactgg ctcaaccctc ggatgaacat gatgtggccc tgatcaaagt ggatattccc
     1261 acgcctttac agaaagtaaa aatgaaagat gcggaaggaa ccgttcagcc cggacaatcg
     1321 gtcacggtaa tgggatatcc gggaattgca cccacacagg tcagtgtccg gaactcaaaa
     1381 gatccgttta accccggtat gaaagaagcc acggtgccca ctcctactgt cacacccgga
     1441 aacatcggac gtatcattcc tgccacttcg atgaaagata tgcggtacag cggatttggt
     1501 gattcttacc agttaaccat aaatgcaacc ggatccggta acagcggcgg accgctcttc
     1561 gatggagaag gaaatgtgat aggcatctat tatgccgggg ccaccgatgc acaaggcaca
     1621 cggatcactt ttgccattcc gattaaatac ggtcttcagc tgatggggat ccggaaatca
     1681 taatcactca acttatcctg tgtgatcagc cgtttctgca atgactgacc ggctgctcac
     1741 acaggtactt tcaacaacca aatatgttca tccccgcaca taccgaaatt gctggttacc
     1801 agctcggaga taaaatcggt accgggggga tgggcgaagt atacagtgcc tggcatccgg
     1861 tgctccgccg taaggcagcg gtgaaaattt tacacaaaat tgaacacacg gaacgtttcc
     1921 gccaggaagc ttatatccag tcgtccgttg atcatccgca tattgcccgg ctctttgaat
     1981 ttacctatac aggcaaaacc ccatgcatca tcatggagta tgtggaaggt gaaccactgg
     2041 acagctatct cagccggcag ggcaaactgg aaagccagga actgatacaa ctcctgacac
     2101 agatcagcaa agccctcgct tatctccatg aacgggaaat attccaccgg gatattaaac
     2161 catccaattt taagcggctg ccggatggat cggttaagat gctggacttc ggcattgcca
     2221 aacacaaata ttctccccgg ctgacgcaac agggatttgt ggtagggact actgaataca
     2281 tggcacccga acaatttgag cagcaggccg acctgcgttc agatatctgg agcctgggag
     2341 tgatgaccta tgaactggca acgggctacc tgccctttga agccactaac cccgttacac
     2401 tccgggcccg tatcagtaag ggaagtttta gtaacccccg gttatttgta cctgaacttt
     2461 ctgagttgct ccggcaggtg attgaacaat gcctgcggat taacccggca caacggccaa
     2521 cggctgtaca actgcaggct ttgctgaccg gaaaagaact ttcaaaaaac aaagagcaaa
     2581 aaaaatggcc cgtaaaaagc ccggtcctga tttctgtcat ttccggcctg gcaatcatac
     2641 tgttggtatg gctcctgaat acacctgacg tgcccaaaca acccgatcca ccggtgaaac
     2701 cggttagcgg atcggaaata aaaccggcgg aacgcagcat caccatcaat gtgcccgggg
     2761 tggagcatgc cgaattggtt ttccccgacg gaagccggca aactgcgccc ttttccatta
     2821 cagggaaaga cggggaactg gtccggttcc ggattgaagc agcaggctac cgggaaaaag
     2881 aagtgtcctt cgacctgcgc atgggcgctt atacctatga atttgtactg gataaaataa
     2941 acaacgaata atgttctgga aaaaactttt taccaaaaac aaacagtcac ctctaaccga
     3001 aaaaatgccg gctaccgggg atacccccaa cttgcaggtg gtcgtccgga gtgatctcgg
     3061 aaacatccgt accaacaacg aggatacggg cttgttttac cggattgcgg atgaaaaagt
     3121 gatccgcgaa aaaggttgtc tgctgatggt agctgatggg atgggcggcc accaggcggg
     3181 ggaagtcgcc agccggatgg cttctgaaat tatcatccgt gaatatttca gcccgcaaaa
     3241 aaatgggggc attgaaaaaa atctgcgccg ggcatttatc gttgccaata ccagtattca
     3301 ccaggctgct gccagcaata aagaacaaca aggcatgggt accacctgta ccgccctcgt
     3361 ggtgacagac ccggtgattc attttgctca tgtcggagac agccgggcct acctgctgaa
     3421 aaaaggaggt ctcagccgca ttaccgaaga tcatacctat gtacaggaat tggtccgtag
     3481 cggggaaatc accgccgcag aagccgatac acatccccaa cgcaatatcc tgaccaatgc
     3541 gatgggcacg aaagcagaca tgaaagtcga tgcgggccgt tacgcattat tgtttgaaac
     3601 aggtgaccgg ctgatgcttt gttcagacgg tctctatgac tacctcaacg atgatgaaat
     3661 tgccgccttc ctcgggggac atgatcttac tgaagcagcc gagcaaatga ttgtcactgc
     3721 aaaaaaaaga ggaggacatg ataatattac cgtggtactg gcccaaaaaa aatcaggaaa
     3781 ggaagaagtc cctgcccgcg aaacacgcga ctttgacctg cccgttacca aagaacttga
     3841 tttaccatga tcggacaatc tatacagaat taccaggtaa ccgcccacct gggggaagga
     3901 ggaatgggca ccgtgtacaa agccaccgat accgtattgg gacgggaagt ggccttgaaa
     3961 atgctccaca ccccgctgat caaccaggcg cagttcctgg aacggttcag aaaggaagcc
     4021 cgcatcctgg cccagctcct gcatccgaat atagccgtga tctacaattt tatcgagcag
     4081 aacaacagcc attacatggt gatggaatat gtggaaggga aaaacctgga cagcctcctg
     4141 cgtcaacagc gagttctttc ctaccaggtg gtggtgcccg tttttatcca ggcccttgaa
     4201 ggattacatc atgcccataa aaaaggaatc tttcaccgcg atatcaaacc ttccaacctg
     4261 atccttacac ccgatggtac ggtgaaactg atggactttg gcattgcaaa aatggcaggc
     4321 gaacaacgca tgacccaggt taaccgggta gttggcacag tggaattcat ggctcctgaa
     4381 ctcatccagg gaaaagatcc ttccgtggct tccgatatct atgccacggc agtaaccatg
     4441 tatgaactgc tgaccggtaa actcccattt gaaagcacca ccgatttcaa cctgatgcag
     4501 gagatcatgc agaagaagcc gccggctccc gacaaactaa ataccgcaat tcctaagcaa
     4561 ctcagttcga ttctgatgaa agccatggaa aaaaaaccgg agaaccggtt tgccgatgca
     4621 cgcgctttac agctagccct gctcagcagc ttcccggaat tgaaagagtt cagcctggac
     4681 cgtgtcagtg atacagtacc ggccacccaa ttcatcagta caaataaggc acaaccactg
     4741 gcgacacagg tggagatgaa caggacaggt gatcagacca ttcctgctgc attcacttcg
     4801 cggataaagg actggtacag ccggacgaat aaaaaacatt tggcaatcgc tcttgtactg
     4861 gcactgatgc tggtgagtat ggccgtcatt ttactaaccg gaaatgaatc accggctgct
     4921 gttaccgatc agccatccgg accagcaccc acaaccaaca gcacgcaact ggtaacagaa
     4981 aacagaaaca gccaaacaga tcaattaatt ccggtgaatc caggtccgcc tgcagataac
     5041 aatacccccg taaccccacc gtcagataaa gtcgaaccga ttaatccggc gggaaatgat
     5101 aaggaaaaaa agaaggaaac gcccaaacca aaagatacag agcaaggtaa agtaaagacc
     5161 cctgtaacac ccaaagaaga aaaaccggcc ccacccccgg aagtaactac gcctaaagaa
     5221 attcttaaac ccattatacc ctcaccggaa gaaacatcga aaagggcacc ccggactgtt
     5281 tcgctgatga caaaaacaga agtaagcctt tacctgcaac aatccatcaa tgcgtccacc
     5341 gcctttgaag gacagggatt gagctttacc gtcacccggc cggtttatta caatggcgaa
     5401 atgataattg aaaaaggcgc caccgctacc ggacggatca ccaaagcggg aaacaagaaa
     5461 ctgggactgg tcatcagcac cgtgaccgcc gccaatggcc agcggatccc gttgcaggaa
     5521 acggaactca gtggaagaat tgaagagatt ttatcgagcc gtaattattc cgctttcctg
     5581 aaaaaaggaa tcaccataac attataacac acaaatcaac tacaatgaaa aaaatcacat
     5641 gcatgctgtt cctgctggtc tcactcgctg ctgatgccca gatcaaacaa cgctccacca
     5701 cgaaagggtt ttctgccgcg gttcaggtac atacactggg ctggacttct gaatatttta
     5761 aatacctgga tgaaggggcc ccgaacggtt tcggcggagg catccggctg ggatacggaa
     5821 ttaacgaaat gatcgagccc tattttgcct atgactttac caaaataaac ttaggagata
     5881 ttgatgcaca gtcctataaa atgaatcata ttgacctggg agtacgcctg aattttgtag
     5941 ggaatgtgca tcctgtacgt ccttttgtgg aagcagggta tacctatctc aaaggcacaa
     6001 tcaaacaggt aatcgccgga tcctcttatg tagatgcgga attctatggc ggtaaacctc
     6061 acctgggagg cggcgtcagc tttttccctt ccatacccat taatcttttt gccaaaggta
     6121 tttttacagt cgggaaaaaa tcaaacctga agcaggatgg tattgttgat accgacaaac
     6181 ccgatgtaac cacttttcgt atcacagtgg gtattggatt taatatccag gaactcatga
     6241 ataaataatt gcaaaccggt ctggaatgag gagggtgcat tgacctttct cattccgctt
     6301 ccggtcatcc ggcttttaca gccttcagtg aatagaccat cggcagttta ccttccatgc
     6361 cctttatctc ccatttaccc gtcgttgttt ccgtcatatt acggaaacag ctatatggtg
     6421 aaaacatatg ctcattaaac agctccagtt tcagaccggc cctgatcagc gcattcagca
     6481 cttcactgat actgtgattc cagctgtact ccttgccttt aatatccgca ttgcggtccg
     6541 tataggttcc ctggttttcg gtgatgatca cttcccggtt ttcatacgaa tactgaatcc
     6601 gggtgaaatc atcatcaaac atccacagaa caggatgaaa ctcggccata taaaattgtc
     6661 ccccgggttt gaggtatcgg gcaatcagac cggcccattt atccaggtca ggtaaccagc
     6721 caatggttcc atagctggta aatacccggt caaaggtttc atccaggtgc gcttccagat
     6781 catatacatt acaacagata aaacgggccc gggttccgat ctcatcgctc agctgctttg
     6841 cctgccggat cgcttcatcc gataaatcca ccccggtgac ctcagcgccc atccgggcca
     6901 ggtccagggt atccattccg aaatgacatt gcaggtgcag gagggttttc cctttcagat
     6961 cctccagctc acccaattca atcggggtaa gcacccgttc tccttttaaa aaaccttcgc
     7021 ggttataaaa agatgaatcg cggtgcgcca tggtccgttg attccagagt tgacggttgg
     7081 cttcgaaata gtcattatac acttccataa tcgttcacca ggtttttttg atactaaaat
     7141 agcatccggt ccgtgacctg aatcactaaa gataaatttt attccttaac gggtatcccc
     7201 caaccgaaaa aacagttcat taacgccggt tatgcgtgat atatgtcacg gcagtaacgg
     7261 gcccgcgcgg atagttttgc ccaaaattaa tcgcgcatga agtatacacg gagacaacaa
     7321 ctgaaccgga tggccgggct gatcacaggt ttattcctgt ttgcaactgc agccctcgcc
     7381 cagcaaccgg ttgttttttt aaccctgaaa gaggcgactg acagtaccct ggcgaataat
     7441 cgggacctgg gcctgtccag actggatgaa aaaattgcga aggcaaagac aaaagaaatg
     7501 gatgccatct accttccgca ggcagacttt tctttcaccg cattaagttc caataatccg
     7561 ctgaatgcct ttggtttcaa actacagcaa caggcggtac agcaaaagga tttcaatccg
     7621 gacctgctga ataaacccgg tgcctccggt gattacagtg gccggctgca ggtgaaacaa
     7681 cccctgttga atatggatct cgtgtacatg cgcaaagccg tagccgccca ggaaggtatt
     7741 taccagatga agacccaacg tacaactgag tacctcgcct atgaagtgaa aaaagggtac
     7801 ctgcaactac agcttttata tgaagcggaa aaagtattaa aggaagcctt acgcagtgcg
     7861 aatgccatgt acaactttac cgataaccgg gtaaaagaag ggctgatgca gaaatcagat
     7921 gccctgaacg taaaagtatg gatccacacc gtggaaacta atctcgccga aacaaccagc
     7981 aatatcggaa atgcctctga ttacctgagc ctcctgatgg gacgccctta tggaacggtc
     8041 tataccgccg aaccggtaca aactgaaaat accgacctga gcggtgccag tgtgccggca
     8101 aacagggcag attttgccgc catgaaaaaa gcaatggaag ccagcagcct gatgataaaa
     8161 tcagcggaga aaagttacct gccccgcatc aacgcctttg cctcctacca ggtgaatgat
     8221 taccggctga ccggttttgg tgccggttct tatctcgccg gactgcaatt atcctgggat
     8281 atttttaaag ggaacagtac aaagaataaa atcattaccc agcagaccga acttagtaaa
     8341 ctgtccggtc aattggaaaa ccagcaggtg caaagccagc tcgaattgaa caaaaccctt
     8401 cgtcagctcg aagatgcccg gttcaaaata aggcagcaaa ccgccgctat tgataatgcc
     8461 actgaagcat tacgcattct gcaggaccgc tatgaacaag gactggtcaa cagtaccgat
     8521 gtcctgcagg ctcagacaca actttctcaa cagcaactcg gcaaggcaca agcggtattt
     8581 agttataata gtactgccgc ctatatcagt ttcttaacct ccacacaaaa aaattaaacg
     8641 cataaatatg tcttacaata agtttatacc aggtgtactc ctcctcagcg ggatcctcct
     8701 catcagttcc tgcaaaggga aaaaagaaac acccgtctcc aaagacgctg cccctgtaac
     8761 cgtttacacg gctactcccg gtggcaccgt ggaaggtgga atcgcggtca gcggaaaagt
     8821 ggaagcactg caaaccgccc agctcagtac caaactgatg ggaacgatca cccgcatctt
     8881 tgtaaaaacc ggtgaccggg tgcgccgggg tcaaaccctc gctacgatca gcagtcagga
     8941 tatcacggcc aaaagagccc agaccagtgc cgctatcctg gaagctgaag ccaatgtaaa
     9001 aaatgcacaa aaagactttg aacgttttac caatctgtat aacaagcaaa gtgcttcagc
     9061 caaggaactc gacaatgtaa ccctgcagta caacgccgcg aaagcccgcc tggaagcggc
     9121 cagccagatg aagaatgaag tcaatgccat cgcctcctac tctacgatca cagctccttt
     9181 tgacggggtg gtgacgcagc ggatggctga tgaaggcaat atggctaacc cgggtatgcc
     9241 cctgctggta gtggaacaaa acacgcagct gcaaatcagt gctaccgtga gtgaagacca
     9301 ggtaggactt ttgaaaaaag gaagtaaagc gcaggttgaa atcaaagcag ctggtaaaag
     9361 catcgagtgt gtggtgaatg aaatcagtcc atcttcacaa atgaccggcg gacaatttga
     9421 aatcaagctc agcattcctg aatcccagaa aaaagatctg tatgcgggga tgtatgtcaa
     9481 tgtatttatt ccggtaagtg gtgctgcagt aagcagccag ccttccggta tagtactggt
     9541 acccacatcc agcctggtgc agaatgacca gctcaccagt atctatacca tcagcagtaa
     9601 taataccgcc ctgcttcgtt gggtcagaac cggcaaggtc tatggcgatc agacggaaat
     9661 actctccggt ctcgcggcta ccgaaaccta tataacaaaa gcggaaggaa agctctacaa
     9721 tggtgtacct gtaaccgtaa aaaaataatc aacccgtaaa cgaattatat gcaagaagga
     9781 atttcaggta aaattgcgcg gtcgttcatt
//
LOCUS       DAOPMW010000087         9758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7581_length_9758_cov_8.530661, whole genome shotgun sequence.
ACCESSION   DAOPMW010000087 DAOPMW010000000
VERSION     DAOPMW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_9758_cov_8.530661"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLU11_06170"
     CDS             complement(<1..1107)
                     /locus_tag="PLU11_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HPN58736.1"
                     /translation="MRKFTLLCLVLATCCFCNANDGSFYASGNTLIPLKETTIQMKKE
                     ILSLERRGNWMQVDIYFEFFNPGPEKELTVGFVTPPAEGDVTDEEAGHPQVKDFMVMA
                     GDRLLPYKMAKMEESGFKVAEKMANGFDFVYYFTIRFAKGITVIRHSYLYRGGGSVEA
                     MADFDYRLTTGTSWAGGAIGDFELNIDMGEDSYYAVPSAFTKTPAGWTMVGVGRIGKI
                     QQYNPYGEEDGGHSLRMVMQRRGKLQLRSKDFRPVKDLSIVVFQLHNEMNLWMENKEK
                     DELATLRDIIWEEHTDSLIRSLTDFQLRLFRNLNYARAGYAFKDESLTKAFLKYTWYI
                     PDPAFKPEQAPEIYIPKEIMKKIVEEEGRRKESGV"
     gene            1204..3312
                     /gene="metG"
                     /locus_tag="PLU11_06175"
     CDS             1204..3312
                     /gene="metG"
                     /locus_tag="PLU11_06175"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPN58737.1"
                     /translation="MSLPKRYLITSALPYANGLKHVGHLAGAYIPADIYARYLRAQKR
                     DVVFVCGSDEHGTAIPIQATKEGTTPRAIIDKYHAAMKQDFEDLSMSFDIYHRTSEPI
                     HHETAQEFFTYLNDRGELEIKETEQYFDEEANSFLADRYIKGTCPNCGSDRAFGDQCE
                     TCGRTLSPDELINPVSTLSGKAPIKKKTSHWYLPLGKHEAFLREWILEQHKDDWRPTV
                     IGQCKSWIEGGLQSRAVTRDLDWGIKVPVAGADGKMLYVWFDAPIGYVSATKQWAIDN
                     NKDWKPYWYDKDTKLVHFIGKDNIVFHCIIFPIMLKLHGNILPENVPANEFLNLEGDK
                     MSTSRNWKLDMRDYINDFVKKENGGGQCVDMLRYYLTQIAPETKDSEFMWKGFQDAVN
                     SELVSKFGNFVNRTWVLMHKLCGGKVPRLHEELLDENDRTLFTDIKDAKTKIENLLEE
                     YKFREALFEVIELSRKGNQYMQKKEPWIVAKKLAENPESQKSIDNTLHICLQLTANLA
                     ILANPFLPNTARKMLHLMKVVEKMLDWDNAGKINLLSVGYTLREPQLLFRQIDDEEIK
                     TQIEKLKSGLLKMEEPKKEEVKPEVPAKAVIQFDDFAKIELKTGTILTAEKVEKADKL
                     LKLSVDLGNEVRTIVSGIALHYQPEEIVGRQVTVVTNLAPRKMRGIESNGMIVMAEDK
                     AGKLHFISPAQPIDPGSTVS"
     gene            3435..7262
                     /locus_tag="PLU11_06180"
     CDS             3435..7262
                     /locus_tag="PLU11_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58738.1"
                     /translation="MKITGIITGTLLLLFFAFHTWINYNARHIIEDLVESRSKGRLKM
                     KLEKFRFSWFSRKVELTNAVIYTADSINATTSYRFQVQHIKLSVQKIWPIVFENKLLI
                     DSLNLDQPEIVVTRLKRLPTDTGSKKEVSIPEELGKIYSSIQDALQALKVNRFQIDNA
                     KFTLLNKIRPDQLPLSISNIHFHIDNLSVDTSTLKGNEKLFFSDNVVLNARNQDIVFP
                     DGRHRLAFSNFRINLKNRLVEFDSCTMTTEQIDRGRSRFRVFFDTLRLANIDFDTLYR
                     SEVIKADTVYCLNPRFDLEVENNSQRKPGEKRTAPTLEEIVQQLTGDLLLGHVIVKNA
                     DFHIKTNFDQHPASFEFTQNNFELEGLKVSQHAPRPLTVKTVALAIRNYENFIRDSTY
                     KLKFDSIVIRENRVYLDRFEFEKRAGGKITNRFSIPRFELSGLSWDDLVFNQQLKAKE
                     ATLYHPSIDYTAVTTASTKGKGKKSIFSTLAEIGQLIQLEKMNIVDGKIDISLKNNTA
                     IQLEDASMTIRTKTLFLAKKARAIEHAVDYLEFSTGRIKSGKLEIQLDDVQYTGNKNG
                     FLLARKVRVKDRESRMDISAENAAVDDILTDEETGDIAASGIRWDKADIWLLLAPPGN
                     TPSKQPAIELKDLSGANTRVLISTGNKTIRSFIKEVKLEELISEDGKPLYLKNPVISG
                     EYAELKDSITHFLVKKFSIEDKRLSVLNEVTYEHTRQQNSTRLLIPSLSVIPDINEML
                     SGRYVMDQLVLNKPVLTAGLSDKGAKAFLPPTRIGHLLITQPEIRFSLADRNGSIRID
                     WNGAIEKDNKVEIRQLEVSQDQPELRLQSLEMNLDHFRYQSPKGKHYDAGSGKIETVI
                     NKLVYQPAADEPANWSATVSKLDARQFIIDSIGKKNGILSVESAAIRDLEINSEILTD
                     PYQLVAGNTRFRLERFTGHYRDAVKQFNWQHAGYSQLTHMVSVDSFAYRHSLDLDSFI
                     SRQQYQKDYIAVGSGPMLIGPVDRQRFLQDSVFQIGKIKIERAWFTDYKDKRLPFAAG
                     NIRFLPVNMIKRIPFKIAADTIQVNPGMVDYTEFNNKTNASGTIPIRRMDITLFDVKN
                     HGIRRNDSLTIRATGYVMDSIWVQLRLKESYTDSLGGFKMTLRAKAGNLAILNPVLIP
                     LVSAKLISGEVDTLHMRVVGREYLAIGEMQMNYKNLKFRYLKDGKDEKQRFFTRLKNY
                     IANTFIIRKNNSSRNGHVFALRARDRSAINYLLRITLSGMSSSAGVTKNKKLYRRYLH
                     ELEKKGLPPPGYE"
     gene            7369..9747
                     /locus_tag="PLU11_06185"
     CDS             7369..9747
                     /locus_tag="PLU11_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HPN58739.1"
                     /translation="MKRAIKKVAVFGSGVMGSRIACHFAGVGVQVLLLDIVPKDVAAD
                     AKPAERNKIVNDALTAAIKSNPSPVYHKDVVKKISTGNFEDNMKDIAGCDWIIEVVVE
                     RLDIKQKVFEQVEQYRKPGTLITSNTSGIPIHMMAEGRSDDFKKHFCGTHFFNPPRYL
                     RLLEIIPTPYTDPEIVDFLMNYGDLYLGKTTVLCKDTPAFIANRIGVFGMMAIMNVKE
                     KLGLTIDEIDALTGPVIGRPKSATFRTADVVGIDTLVKVAKGVADNCPADEAIDQFAI
                     PAWLDKMVSNNWLGDKTGQGFFKKTKGAGGEKEILTLNLATMEYAPRVKPKFATLEAA
                     KPIDDLNARLRVLVAGQDKAGEFYRLFHYGLFSYIAHRIPEISDELYRVDDAMMAGFG
                     WEIGAFESWDVLGVAKTTEAMKAAGYKVAPWVEEMLAAGNKSFFKIEAGKKLSYDPAS
                     KTYKTLPGGEAFIVMSNQTDKQIWKNSSCRLYELGDDVLGLQWFTKMGSIGSDVLSGI
                     QTAIDKAEKNYKGLVIANEGANFSAGANVGMIFMFAIEQDYDELNMAIRFFQQTMMRA
                     RYSSIPVVVAPHGLTLGGACELNLHADKCCPAAETYTGLVELGVGLIPGGGGTKEFVL
                     RASDEMHEDEPETITLKNRFLSIATAKVATSAHEGFGLGIYRKGLDEVVMNQGRRIAE
                     AKKSVIEIFDSGYTAPVQRNDIKVLGQSALGALYSGIHAMKTANYATEHDALVAKKLA
                     YVMCGGDLSEPTLVSEQYLLDLEREAFLSLCGEKKTLERIQSVLKSGRPIRN"
BASE COUNT         2767 a         2237 c         2353 g         2401 t
ORIGIN      
        1 aactcccgac tctttcctcc tcccctcctc ctccactatc ttcttcataa tctccttcgg
       61 aatataaatc tccggagcct gttcaggttt gaaagcaggg tcggggatat accaggtata
      121 ttttaaaaag gcttttgtta atgattcatc tttgaaggca taacccgccc gggcatagtt
      181 aaggttgcga aatagccgaa gttgaaaatc agtcaatgat cgaatcaaag aatcggtgtg
      241 ttcctcccat ataatatcac gtaaagtggc gagttcgtcc ttttccttat tttccatcca
      301 gaggttcatc tcattgtgca gctggaatac cacaatagag aggtctttta ccggcctgaa
      361 gtccttgctt cgcaactgca gctttccccg gcgctgcatc accatccgaa gactatgccc
      421 gccatcttcc tcgccatatg gattgtattg ttggatctta ccaatacgcc ccacccccac
      481 catggtccag ccggccggtg tctttgtaaa agcggaaggc actgcataat aactgtcttc
      541 tcccatatca atattcagtt caaaatcacc gattgccccg ccggcccatg atgtgcccgt
      601 ggtcaggcgg taatcgaaat cagccatggc ttccacagat cctcctcccc ggtataaata
      661 actgtgccgg ataaccgtaa tacctttggc aaagcggatc gtgaaataat agacaaaatc
      721 aaatccattg gccatttttt cagccacctt aaatccggac tcttccattt tagccatttt
      781 atatggaagc agccggtccc cggccatcac cataaaatcc ttaacctgcg gatggcctgc
      841 ttcctcatcc gtcacatccc cctctgcagg tggtgtaaca aatccaaccg tcagctcttt
      901 ctccggaccc gggttaaaaa actcgaaata aatatccacc tgcatccagt tgccccgtcg
      961 ttcgaggctc aggatttctt ttttcatctg aatggtggtt tcctttaaag gaataagggt
     1021 attgccagag gcatagaagg agccgtcatt ggcgttacaa aagcaacaag tggctaaaac
     1081 caggcataaa agggtgaatt ttcgcattac agtcgggttt gtacccctaa gttaccccaa
     1141 tcatggtttt gggcaatgcc cgtcaatacg gtaaatttgc aaccgatcaa agaaacttgt
     1201 tgaatgagct taccgaaaag atacctgatt acctccgccc tcccttacgc caacggcctc
     1261 aagcacgtgg gacacctggc cggtgcctat attccggctg atatctatgc ccgttacctg
     1321 cgggcgcaga aaagagatgt ggtgtttgtc tgcggcagtg atgaacatgg cacggccatc
     1381 cctatccagg ccaccaagga aggtactacg ccccgtgcca tcatcgacaa ataccatgca
     1441 gccatgaaac aggactttga agacctgagc atgagctttg atatctacca ccgtaccagt
     1501 gagcccatcc accatgaaac ggcacaggaa ttttttactt acctgaatga ccggggagaa
     1561 cttgaaataa aagaaacgga gcaatatttt gacgaagagg cgaacagctt cctggccgac
     1621 cgctatatca agggtacttg ccccaactgt ggcagtgacc gtgcttttgg agatcaatgt
     1681 gaaacctgtg ggcgtaccct gagtccggat gaactgatca acccggtcag tacgttaagc
     1741 ggaaaggcac ccatcaagaa aaaaacctca cactggtatc tgcctctcgg taaacatgaa
     1801 gcgttcctgc gcgaatggat actggagcaa cataaagacg actggcgtcc gactgtaatc
     1861 ggccagtgca agagctggat cgaaggcggc ctgcaatcaa gagccgtaac ccgtgacctg
     1921 gactggggga ttaaagtgcc tgtggccggg gccgatggca aaatgctcta tgtttggttt
     1981 gatgcaccga tcggttatgt gagtgccacc aaacaatggg ctattgacaa taacaaagac
     2041 tggaagcctt actggtatga taaagacacc aaactggtgc attttattgg caaagacaat
     2101 atcgtattcc attgcatcat cttcccgatc atgctgaaac tgcatgggaa tattttaccg
     2161 gagaatgtac cggccaatga attcctgaac ctggaaggcg acaagatgag taccagccgc
     2221 aactggaagc tcgatatgcg ggattatatc aatgattttg tcaaaaagga aaacgggggc
     2281 ggtcaatgcg tggatatgtt gcgttattac ctgacccaga tcgcgccgga gaccaaggac
     2341 agtgaattta tgtggaaagg attccaggat gcggtgaaca gtgaactggt ttccaagttc
     2401 ggcaactttg taaaccggac ctgggtgctg atgcataaac tctgcggggg taaagtacca
     2461 cgcctgcatg aggaattgct ggatgagaat gaccggaccc tgtttacgga tatcaaagac
     2521 gctaaaacaa aaattgaaaa tctgctggag gaatataagt tcagggaagc gttgtttgaa
     2581 gtgattgaac tttcccggaa aggcaatcag tatatgcaga aaaaagaacc ctggatcgtg
     2641 gccaaaaaac tggcggaaaa tcccgagtca cagaaaagta ttgataatac tcttcatatc
     2701 tgtttgcaac tgaccgccaa tctcgcgatc ctggccaatc ccttcttgcc gaatacggca
     2761 cggaaaatgc tgcatctgat gaaagtggtg gagaaaatgc tggactggga caatgcgggc
     2821 aagataaacc tgctgagtgt gggttatacc ctgcgggaac cccagttatt attccgtcag
     2881 attgatgacg aagagatcaa aacacaaata gaaaaactta aaagcgggct tttaaaaatg
     2941 gaagaaccta aaaaagaaga agtaaaaccg gaagttccgg ccaaagccgt catccagttt
     3001 gatgattttg caaaaattga actgaaaacg ggtaccatcc ttacggcgga gaaagtggag
     3061 aaggcggata aactgctcaa attgtcggta gatcttggca atgaagtccg cactattgtg
     3121 agcgggatag ccctgcatta ccaacccgaa gaaattgtgg gccgccaggt aacggtggtg
     3181 accaatctgg caccgcgtaa aatgcgtgga attgagagca acgggatgat tgtgatggcg
     3241 gaggacaagg ctggcaaact gcattttata tcacctgctc agccaatcga tccgggttct
     3301 acggtcagtt aattgttaaa atactgtaag cccccgtaat aacgggggtt ttccttttct
     3361 gcggcaacga attcagtaat tttatacaaa gagggagcca caagttgaca aagaaaagcc
     3421 gcggtagaaa attattgaag attaccggta tcattaccgg taccctgctc ctgttgtttt
     3481 ttgcttttca cacctggatc aattacaatg cccggcatat tattgaagac ctggtagaat
     3541 ccagatcgaa ggggcggtta aaaatgaaac tggaaaaatt ccgtttcagc tggttcagcc
     3601 gtaaagtgga attaaccaat gcggtcatct atacggccga ctccatcaat gccactactt
     3661 cctaccgttt ccaggtacag catataaaac tttccgtaca gaaaatctgg cccattgttt
     3721 ttgaaaacaa gttgctgatt gactcactca acctggacca gccggaaatc gtggtgaccc
     3781 ggctgaagcg attacctacc gatacgggta gtaaaaaaga agtatcaata ccggaagaac
     3841 tcggcaagat ctattcgtcc attcaggatg cgttgcaggc tttaaaagta aaccgcttcc
     3901 agatcgacaa tgcgaaattc acgttgctca ataaaatcag gccggaccag ttacccttat
     3961 cgatcagtaa cattcacttt cacatcgata acctgtcggt cgataccagt accctcaaag
     4021 gaaatgaaaa actttttttc agcgacaatg tagtcctcaa tgcccgcaac caggatattg
     4081 tttttccgga cgggagacac cggttggcct ttagtaattt ccggatcaac ctgaaaaaca
     4141 gactggtgga atttgacagc tgtacgatga ccaccgaaca aatagaccgt ggccgttcgc
     4201 gtttccgtgt tttcttcgat accttacggc tggccaatat agattttgac accttgtacc
     4261 gttccgaagt aatcaaggcg gacaccgtgt attgcctcaa tccccgcttt gacctggaag
     4321 tggagaataa cagccagcgc aaacccggtg aaaagcgaac cgcccccaca ctggaagaga
     4381 ttgtacaaca gctgactggt gacctgctgc tggggcatgt gattgtgaag aatgccgact
     4441 ttcatatcaa aacaaatttt gaccagcatc cggcttcgtt tgaatttacc caaaacaatt
     4501 ttgaactgga gggactgaaa gtatcccagc atgctccccg gccgttaacg gttaaaaccg
     4561 tggccctggc gatccgtaat tatgaaaatt ttatccgtga cagtacctat aaattgaaat
     4621 tcgacagtat tgtgatccgt gaaaaccggg tctatctcga ccggtttgaa ttcgagaaaa
     4681 gggccggcgg aaaaatcacg aaccgtttca gtattccccg gtttgaactg agcggtttgt
     4741 cctgggatga cctcgtgttt aatcagcagc taaaagcaaa agaagccact ttatatcacc
     4801 cgtccattga ctatactgct gtcaccaccg cctctacgaa aggcaaaggg aagaaaagta
     4861 ttttttccac cctggcggaa attggccagc tgatacaact ggagaaaatg aatattgtgg
     4921 atggcaagat tgatatttcc ttaaaaaaca ataccgccat tcagctcgaa gatgcttcga
     4981 tgacgatccg taccaagacc ctcttcctgg caaaaaaagc gcgggctatt gaacatgctg
     5041 tggattacct ggaattttcc actggtcgga taaaatcagg caaactggaa atacaattgg
     5101 acgatgtaca gtacacgggt aataagaatg gttttttgct ggcgcggaaa gtccgggtaa
     5161 aagacaggga aagccggatg gatatttcgg ctgaaaacgc agcggtggat gatatcctga
     5221 ccgatgaaga aaccggcgat attgcggcca gtggtatccg ttgggacaag gccgatatat
     5281 ggttattgtt agccccgcct gggaataccc cttcgaaaca acccgcgatt gaactgaaag
     5341 atctttccgg ggctaacacc cgggtattaa tcagtactgg caacaagacc atccggtcat
     5401 ttatcaaaga agtaaaactg gaagagttga ttagtgagga tggcaagccc ttgtatttaa
     5461 aaaatccggt catcagtgga gagtatgccg aactgaaaga cagtatcact cattttttag
     5521 tgaagaaatt cagtattgaa gataaacgcc tttccgtttt aaatgaagta acctatgaac
     5581 atacgcgtca gcaaaacagc acccgtctgc ttattccttc cctatcagtt attccggata
     5641 ttaatgaaat gctgagtggc cgctatgtga tggatcaact ggtattaaat aagcccgttc
     5701 tcactgccgg tctgagtgat aaaggagcta aagcattcct ccctcccacc cgtatcggac
     5761 atttgctgat cactcaaccg gaaatccgtt tcagtctggc tgacagaaac ggcagcatcc
     5821 gtattgactg gaacggtgcc attgaaaaag acaataaagt agagatccgt caactggaag
     5881 tcagccagga tcaacctgaa ctccgtttac aatcactgga aatgaacctg gatcattttc
     5941 gttatcagag tccgaaaggc aaacattatg atgctggctc agggaaaata gaaaccgtaa
     6001 ttaacaagct ggtctatcaa ccggcggctg atgaaccagc caattggtcg gctacggtaa
     6061 gtaagttgga tgcccgccag tttatcattg acagtatagg taagaaaaac ggcatcctga
     6121 gtgtggaatc tgccgccatc cgcgacctgg agatcaatag cgaaatactc actgatcctt
     6181 atcagctggt ggcgggtaat acccgctttc gtctcgaacg atttaccggg cattaccggg
     6241 acgcggttaa gcaattcaac tggcagcatg ccgggtatag tcagcttacc catatggtat
     6301 ccgtggacag ttttgcctac cgacatagtc tcgacctgga ttcctttata tcccgccagc
     6361 aataccagaa agattatata gctgtcggga gtgggccgat gctgatcggg ccggttgacc
     6421 gtcaacggtt tttgcaggac agtgtgttcc agattggtaa aataaaaatt gaaagagcct
     6481 ggttcacgga ttataaagac aagcgattac cctttgccgc cggtaacatc cgttttttgc
     6541 ctgtcaatat gattaaacga attcctttta aaatcgcggc agatacgatc caggtcaatc
     6601 ccggcatggt ggactatacc gagttcaaca ataaaaccaa tgcgtcagga accattccta
     6661 tccggcgcat ggatatcaca ttatttgatg tgaagaacca tggcatccgg aggaatgaca
     6721 gtcttactat ccgcgcaacc ggatatgtaa tggattccat ctgggtccag ttacggttaa
     6781 aagaatccta taccgattcg ctcggcggct ttaaaatgac tctgcgggcc aaggcgggta
     6841 acctggccat attgaacccg gtgttgattc cgctggtatc cgcgaaattg atttccggtg
     6901 aagtagatac tttacatatg cgggtagttg gccgggaata cctcgccatt ggtgaaatgc
     6961 agatgaatta taaaaatctg aaattccgct acctcaaaga tgggaaagat gaaaaacaac
     7021 gattttttac ccggttgaag aactacatcg ccaatacctt tatcatccgg aaaaataaca
     7081 gttcccgaaa cgggcatgta tttgcgctcc gggcccggga ccggtcggcc attaactacc
     7141 tgctccgcat taccctgagc ggaatgagca gtagtgccgg ggtcaccaaa aacaagaaac
     7201 tgtaccgcag gtacctgcat gaactggaga aaaaaggcct gcctcccccg gggtatgaat
     7261 aagctataaa cccggttttt ccagttcaca aaaaatctta tcttcgattt caaattagtt
     7321 gcataactaa ctaattttgt attttacctc tttcataaaa ctgaatctat gaagcgtgcc
     7381 attaaaaaag ttgctgtctt cggaagtggt gtaatgggtt cccgtattgc ctgtcatttc
     7441 gccggtgtag gtgtacaggt attgttgctt gatattgtgc ccaaagatgt agccgccgat
     7501 gccaaaccgg ctgagcggaa taagatcgta aatgatgcac tgacagccgc tataaaaagc
     7561 aacccctccc cggtgtacca caaggatgtg gtgaaaaaaa tcagcactgg caattttgag
     7621 gataatatga aagatattgc cggatgtgat tggattattg aagtggtggt ggaacggctg
     7681 gatatcaaac aaaaagtatt tgaacaggta gaacagtacc ggaagcccgg aaccctgatt
     7741 acttccaata catcgggcat tcccattcat atgatggcgg aagggcgcag tgatgatttt
     7801 aaaaaacatt tctgtggcac ccactttttt aatccccctc gctacctccg tcttttggag
     7861 atcataccca ccccctatac cgatcctgag atcgtggact tcctgatgaa ttacggggat
     7921 ctctacctgg gtaagacgac tgttttatgt aaagacacac ccgcttttat cgccaaccgg
     7981 attggcgtat tcgggatgat ggcaatcatg aacgtaaaag aaaaacttgg attgacgatt
     8041 gatgagatcg atgcattgac cggtccggtc attggtcgtc ccaaatccgc gactttccgt
     8101 accgccgatg tggtggggat cgatacattg gttaaagtgg caaagggagt agctgataat
     8161 tgtccggcgg atgaagccat tgatcagttt gccattccgg cctggctgga taaaatggtc
     8221 agcaataact ggctgggtga taaaacgggg cagggctttt ttaagaaaac aaaaggagcc
     8281 ggcggcgaga aagaaattct caccctgaat ctcgcgacga tggaatatgc cccgcgggtt
     8341 aagccaaagt ttgccaccct ggaagccgct aaacccattg atgatctcaa tgcgcgttta
     8401 agggttttag tagccgggca ggataaggcc ggtgaatttt accgtttatt ccattatggt
     8461 ttattttcct atatcgctca ccgtattccg gaaatcagtg atgaactgta ccgggtggat
     8521 gatgcgatga tggccggttt tggctgggag atcggtgctt tcgaaagctg ggatgtgttg
     8581 ggtgtggcga aaaccacgga agcaatgaaa gcggcgggtt ataaagtagc gccctgggtg
     8641 gaggaaatgc tggcggccgg taataaatct ttcttcaaaa ttgaagccgg caaaaaactc
     8701 agttacgatc cggccagcaa aacctataaa acattacccg gtggtgaagc ttttatcgtg
     8761 atgagcaatc agaccgataa acagatctgg aaaaacagca gttgccggct ctatgaactg
     8821 ggtgatgatg tactcggact gcaatggttt accaaaatgg gcagcatcgg tagtgatgtg
     8881 ctcagtggca ttcagaccgc gatagataaa gccgagaaaa attataaagg cctcgtgatt
     8941 gccaatgaag gcgccaactt ttcggccggc gccaatgtag gcatgatctt tatgtttgcc
     9001 attgaacagg attatgacga actgaatatg gccatccgct tcttccagca aacgatgatg
     9061 cgggcccgtt attcatccat acctgttgta gttgctcctc atggtttaac cctgggcggc
     9121 gcttgtgaat taaacctgca tgcggataaa tgttgtccgg ctgcggaaac ctataccgga
     9181 ttagttgaac ttggtgtagg tctgattccc ggcggtggag gcaccaaaga atttgtgttg
     9241 cgtgcttccg atgaaatgca tgaagacgaa cccgaaacca tcaccttgaa gaatcgtttt
     9301 ctttccattg ctacggccaa agtggcaact tcagcgcatg aaggattcgg actgggtatc
     9361 tatcgcaaag gactggatga agtggtgatg aaccagggac gtcgtattgc ggaagcgaaa
     9421 aaatcggtga tcgaaatttt tgacagcggc tataccgcac cggtacagcg aaacgatatt
     9481 aaagtactcg gtcaatcggc cctaggtgcc ttatactccg gcatccatgc catgaagacc
     9541 gccaactatg ccaccgagca tgatgcgttg gtggcgaaga aactcgccta cgtgatgtgt
     9601 ggcggcgacc tgagcgaacc tacattggtg tctgaacagt atctgctgga tcttgagcgg
     9661 gaagcttttc tttcgctatg tggcgagaag aagacactgg aaaggataca gagtgtgctt
     9721 aaaagtggtc gacctataag gaactaatag ctcttgca
//
LOCUS       DAOPMW010000088         9732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7609_length_9732_cov_8.220420, whole genome shotgun sequence.
ACCESSION   DAOPMW010000088 DAOPMW010000000
VERSION     DAOPMW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_9732_cov_8.220420"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..>1240)
                     /locus_tag="PLU11_06190"
     CDS             complement(332..>1240)
                     /locus_tag="PLU11_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HPN58740.1"
                     /translation="DEMLFIVIHQAYELWFKQLHHEVDSVAGILRQPALNDNSPELQT
                     IVHRLSRCVTILRVLVHQVDIMETMTPMDFLDFRDMLRPASGFQSWQFKELEAKLGLK
                     FDHRHGKEYYTSQLKQEHVSVIKEAEAATSLLQLVNDWLERMPFLKGGADATFWNHYK
                     ESYRSSLAEAERNNLSAFDQVFADGSVDTERSLSPAACRAALFIMLYRGYPLLQLPFQ
                     LLNCLLEIDEQLSSWRYRHMNMVHRMIGMRIGTGGSTGKDYLKAAADKHYIFKEIAQL
                     NSFLMERRKLPALPSAVEEVLGFRHG"
     assembly_gap    1241..1340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1341..2374)
                     /gene="purD"
                     /locus_tag="PLU11_06195"
     CDS             complement(<1341..2374)
                     /gene="purD"
                     /locus_tag="PLU11_06195"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPN58741.1"
                     /translation="MRILLLGSGGRENALAWKLTQSPRCEALFIAPGNAGTTRFGTNL
                     DISLTDFEGIRKACMDEKIDMVIVGPEEPLVKGVTDFLISSKSLADLDVIGPGKAGAQ
                     LEGSKAFAKAFMMRHDIPTAAYREFTLDNYTEGVEYLRQHTLPIVLKADGLAAGKGVV
                     IAENHIEAIAEFELMIQRAKFGEAGKKVVVEQFLKGIEISVFVLTDGKNYVILPEAKD
                     YKRVGEGDTGPNTGGMGAVSPVPFVNKELMQKIEEKVIRPTVDGLRHEGLEYRGFIFF
                     GFMIVDNEPYMIEYNCRMGDPETEVVIPRLKNDLVDLLDAVATQQLDKMTIETDPRTA
                     CTVVAVSGGYP"
     gene            2518..4194
                     /locus_tag="PLU11_06200"
     CDS             2518..4194
                     /locus_tag="PLU11_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPN58742.1"
                     /translation="MNQSVKAGKHPKALFVLFFTEMWERFAYYLMVGILLIYMTDSTT
                     GGKGFTGKIGADIVGSFIALVYLTPFIGGLIADRYLGYIKSIFLGGSLMAAGYLGLSL
                     PGDTAMFISLSLIIIGNGFFKPNISTLLGNIYNREDLKPLKDNAYNIFYMGINIGAFV
                     CNFVAAWLRNAYGWGYAFGAAGVGLIIGMVILAGFVNDEDIKKANVKKPVQAEDMPIS
                     TIFMTVFLPAIIAAVIGWILPTKIFGAPVMGTQANDAFIFACLPVIAFYVSLWVRAKG
                     QEKKSIGALLFVFAIAIAFWTIYNQNATGLTLWAEKHTDRVASASTARVTGAIFPLQQ
                     VTDTPRMTNKLDEYMRGVKDDSGNIVQALGPDPYFVNVPKEDWPQGKDVKLTNTELFQ
                     SINPLFIVLLTLFFVPFFSFLRSKGKEPTTMSKFGMALFISGLSALVMVFAIMSVPSI
                     YGHKASPLWLWGTYFVFTISEIFLSPMGLSLVSKLAPARLTSLLMGGWFLSTSIGGKV
                     AGVMTSFWDDFTDKKIFFLILVAGGFIGGILIYSRIKSLNQIVKDKTGAD"
     gene            4300..5952
                     /locus_tag="PLU11_06205"
     CDS             4300..5952
                     /locus_tag="PLU11_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPN58743.1"
                     /translation="MWKKHPKALPFLFFSEMWERFGYYLMIGIFTLYLKDAADGFNMD
                     EKDSASLYGTFIALVFFTPFLGGLIADRYLGYRRSIIMGGLLMGVGYCTMGIHNITML
                     YVSMALVIIGNGFFKPNISTLLGNVYSTPEHLHQKDEGYNIFYMGINVGAFVCNFFGA
                     ALYTMIGWGAAFAAAGVGMFIGVITFIAGTRHYKDFDVRKPLKPGDMPLGKILALVIL
                     PSILFGVIGWFIKGTNGYIFDSNSTDAFIFACIPVCVFYTALLLRSSKDERPAIGAML
                     AIFAVVILFWAIFKQNGSALNTWASRYTDREVTGTTGDFLAALKQTDKAANTTYKLDS
                     VTKLDNQFRKVRDAQGNEVKELGYPSYFKNVAPEKLPAEGAVVKAWLPSLSQSINPFW
                     VIALTPLVVAFFTWLRRKGKEPSTPTKIAFGLLVSALSVLVMVAAVKAGHNGTEKVSV
                     WWLVGSYGVVTIGELFLSPMGLSLVSKLSPVRITSLMMGGWFLSTSIGNKLSGILASK
                     WDQYEDKTDYFWLNFILLGGAAIFLFVLLKWLNRVMKEKGVN"
     gene            6107..8119
                     /locus_tag="PLU11_06210"
     CDS             6107..8119
                     /locus_tag="PLU11_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPN58744.1"
                     /translation="MSEQQKVFRPFVPPEARMAEFTIKSVITGSIFGIIFGAATVYLA
                     LKAGLTVSASIPIAVLSIALGKLFLKTTILENNIIQTTGSAGESIAAGVVFTLPGFLF
                     LSSPDSSSYFNYLTIMTLAILGGMLGTLMMIPLRRSLIVKEHETLPYPEGTACASVLK
                     AGEKGGDLAKTAFMGMGVAIIYAFLQKVFHVIAEVPVYATHLKNKYLPSAKISGEITP
                     EYLGVGYIIGPKISGILVAGGVLSSFVLIPLLATLVPGDATFAQLTKLGLNPAKFGWD
                     ATSHTFTDSAEALYRAYIRQIGAGAVAAGGFITLIKTIPTIISSFKESIGAVKDKSAG
                     KSVIRTEQDLSIKVVGLGSLGLILIMALLSIIPGENIGSRLLLGVLVIIFGAFFVTVS
                     SRIVGLIGSSNNPISGMTIATIMATCLVFIGVGWSGKLYEPMALVVGGMICVAAANAG
                     ATSQDLKTGYLVGATPKYQQLALFIGAIVSSIVIGWTVKILDTPSAALAATGVQHAIG
                     ETYSAPQATLMATLIKGILSFNLDWQFVLIGVFIAIVLELCGVKALSFAVGTYLPLST
                     TLPIFIGGFIRGVADRKAKKDSEPEEEEDLRKGNLFATGLVAGGALFGVIAAILTVFF
                     ESAMKSVNLEEGVTGSFGHSGYMALGVGFFAAMTFILYKVATGKKK"
     gene            complement(8251..9450)
                     /locus_tag="PLU11_06215"
     CDS             complement(8251..9450)
                     /locus_tag="PLU11_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPN58745.1"
                     /translation="MYIKRKKFIQIGSLATASLMVPKFLKAFESPARVMPGNKVVVVL
                     QLSGGNDGLNTVIPYRNDLYYKARPRLGILRDKALSLTDETGLHPALTAFKELYDDGS
                     LSILNNVGYPNPDRSHFRSMDIWHTASQSTEYWSQGWVGRYLDAQCKGCDKPTQAIEL
                     DDVLSLALKGENIKGIAVKDPRRLYGTANEKFFREVMKNHKDEAGEQPVDYLYKTMAE
                     TLSSADYIFQQSRLHPSNADYPKTDLGNSLKTIASLIFSEINTKVYYVSLGSFDTHIN
                     QEAQQQRLFTEMNDAVKSFVKDLKEQHRFEDVLLFTFSEFGRRVEQNASGGTDHGTAN
                     NMFLVSGGLKKKGVLNPMPDLANLNEGDLKYNVDFKNVYATILNKWLQADDVGILGRK
                     YEYLNFI"
BASE COUNT         2402 a         2305 c         2197 g         2728 t
ORIGIN      
        1 atcaacgttt ccatcgactg gcagccctcg tttgagtttg gagataaccg gggatttgtg
       61 gggaattggg ggggactggg aatcaggtat acgctcaaat aaataagaaa taagaaataa
      121 agaataagga atgaggaagt gccttctgaa aagaattagc taatgaatag gacgatactt
      181 gttataaggc agccgtttta aaatgagaaa taaggaacaa aaaataagga atgaggaagt
      241 acctcctgag aaatggttgt ttatttaaac gaccctgctt cttttggatt tactttctaa
      301 ttccttattc tttatttctt attccttatt tctacccatg ccggaagcca agtacttctt
      361 ctaccgctga cggcaaagcc gggagtttcc ttctttccat caggaaacta tttagctggg
      421 caatttcttt gaagatatag tgcttatctg ctgcggcttt cagatagtct ttaccggtac
      481 tgccacccgt gccgatgcgc ataccgatca tacggtgcac catattcatg tggcgataac
      541 gccaggaact gagctgctca tcaatctcga gtaagcaatt aagtaactga aagggcagtt
      601 gcagtaatgg atagccccga tacaacatga taaacagggc ggcccggcag gcagcaggag
      661 ataaagaacg ttcagtatcc actgatccgt cggcgaatac ctggtcaaag gctgagagat
      721 tattccgttc ggcttcggcc agacttgacc ggtagctttc cttgtaatga ttccagaaag
      781 tggcatccgc accacctttc aggaaaggca tgcgctccag ccagtcattc actaattgca
      841 ataaagaagt ggccgcttcg gcttccttga ttaccgatac atgttcctgt tttaactggg
      901 acgtataata ttccttgccg tggcggtgat caaatttaag tccgagtttg gcttccagtt
      961 ccttgaactg ccagctctga aaaccggagg ccggtcgtaa catatccctg aaatcgagaa
     1021 agtccatcgg ggtcatggtc tccatgatat ccacctggtg taccagtacc cgaaggatgg
     1081 tgacacaacg gctgagccgg tgaacaatcg tctgcaattc gggagaatta tcattcagcg
     1141 caggttgccg taaaattccg gccaccgaat ccacttcatg gtgcagttgt ttaaaccaga
     1201 gttcgtaggc ctggtgaatc acaataaaca gcatttcatc nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn ggataaccgc cactgaccgc caccaccgta caggcggtgc
     1381 gtgggtctgt ttcaattgtc attttatcca gttgctgggt agctacggca tccagcaggt
     1441 ccaccaggtc gtttttcaac cggggaatca ctacttctgt ttcgggatca cccatccggc
     1501 aattatactc gatcatgtaa ggttcattgt ccacgatcat aaaaccaaag aatataaatc
     1561 cgcgatattc aagtccttca tgacgcagtc cgtcaacggt gggacggatc actttctcct
     1621 cgatcttctg catcagctct ttatttacaa aaggcaccgg acttacggct cccataccac
     1681 cggtattggg accggtatcg ccctcgccta ctcttttata gtcttttgct tccgggagga
     1741 tgacatagtt cttcccgtcc gtcaatacaa atacagatat ctctatccct tttaagaatt
     1801 gttccaccac cactttcttt cccgcttcgc caaatttggc gcgttggatc atcagttcaa
     1861 attctgcgat agcctcaata tggttttcag cgatcacgac gcctttaccg gcggccagac
     1921 catctgcctt taaaacgatc gggagggtat gttgccggag gtattccacg ccttccgtat
     1981 aattatcaag ggtaaactca cggtaagcag ctgtaggaat atcatgacgc atcataaaag
     2041 ctttggcaaa cgccttactg ccttcgagct gggcaccggc tttaccaggt ccgatcacat
     2101 ccagatctgc cagtgattta ctactgatca ggaaatcggt cacacctttc accagtggtt
     2161 cttcgggtcc tacaattacc atatcgatct tttcatccat acaggctttc cggattcctt
     2221 caaaatcagt cagggaaata tccaggtttg ttccaaatcg ggtagtgcct gcattaccag
     2281 gagcgataaa gagagcttca caacgggggc tttgggtcag cttccaggcc agggcatttt
     2341 ccctgccacc tgagcctaat aataaaatgc gcattcggtc ggttggtttc taagtattta
     2401 caaatatctg tcagagcggt taaagataaa ctgaaaaaac ttgcgggtga ttaaatttct
     2461 gtattttggg gattgctaag gttacgcgac tctgatttac aaactaaaaa ctactgtatg
     2521 aatcaatcag taaaagcggg taaacacccc aaagcgctat ttgtactctt ttttacagaa
     2581 atgtgggaac gatttgccta ctacctgatg gtagggattc tcctgattta catgaccgac
     2641 agtacaaccg ggggaaaagg atttaccgga aagatcgggg ccgacatcgt gggtagtttt
     2701 attgcacttg tttatctgac tccttttata ggtgggttga ttgccgaccg ctacctgggt
     2761 tatattaaat ccatttttct cggaggctca ttgatggccg ccggttatct cggactttct
     2821 ttacccggag ataccgccat gtttatttca ttatccctga tcattatcgg aaatggcttc
     2881 ttcaaaccta atatctccac cctgttagga aatatttaca accgggaaga cctgaagccg
     2941 cttaaagaca atgcctataa tatattctac atgggtatta atatcggcgc ctttgtctgc
     3001 aatttcgtag ctgcatggct ccgaaatgca tatggatggg gctatgcgtt tggcgccgca
     3061 ggtgtcggac tgatcattgg aatggtgatc cttgccggat ttgtgaatga cgaagacatc
     3121 aaaaaagcca atgtcaaaaa accggtacag gcagaagaca tgccgatctc caccattttt
     3181 atgaccgttt tcctgccggc cattatagct gcagtcatcg gatggattct ccccaccaaa
     3241 atatttgggg cgccggtaat gggtacccag gccaatgacg ctttcatttt cgcctgttta
     3301 cccgtcatcg ccttctacgt ttcattatgg gtaagagcca aaggacagga gaaaaagagt
     3361 atcggcgcct tgttatttgt gttcgccatc gccattgcgt tctggacgat ctataatcag
     3421 aatgccaccg gactcaccct ctgggctgaa aagcatacag accgtgtggc ttctgcatcc
     3481 actgcccggg taaccggtgc catattccct ttgcaacagg taacggatac accgcgcatg
     3541 accaacaaac tggatgagta catgcgggga gtaaaagatg acagcggcaa catagtacaa
     3601 gcattagggc ctgatcccta ttttgtgaat gttcccaaag aagactggcc gcaaggcaaa
     3661 gatgtcaaac tcaccaacac agaattattc cagtcaatta atccgctctt tatagtattg
     3721 ctgactttgt ttttcgttcc tttctttagt tttctacgaa gtaaaggcaa ggaacccaca
     3781 accatgtcaa aattcgggat ggccctgttt atatccggac tttccgcact tgtaatggtc
     3841 tttgcgatca tgtctgtccc ctctatttat ggacataaag catcccccct gtggttatgg
     3901 ggtacttatt ttgtctttac catcagtgaa atcttcctga gtccgatggg gctttcactg
     3961 gtctccaaac tggctccggc caggctcact tccttattga tgggaggttg gttcctttct
     4021 acttccattg gcggtaaagt ggccggtgtt atgaccagtt tctgggatga ctttactgat
     4081 aaaaaaatat ttttcctgat cctggttgcc ggtgggttta ttggaggtat cctgatttat
     4141 tcaagaatca aatcactcaa tcagattgta aaagataaaa caggagccga ctaacaggtc
     4201 cggcttctta acaaaaaagg cagcaccctc ccaggttgct gccttttttg ttttccctat
     4261 gctttccttt attatctttc catatcaaac gaaattgtta tgtggaaaaa acacccgaaa
     4321 gccctgccct ttcttttctt ttctgaaatg tgggaacgct ttggctatta cctgatgatc
     4381 ggcatcttca ccctctacct caaagatgca gcggacggtt tcaatatgga tgaaaaggat
     4441 tcagccagcc tgtatggtac ctttatcgcc ctggtgtttt ttacgccctt cctgggcgga
     4501 ttgattgcgg accgttacct cggttaccgc cgttccatca ttatgggcgg tttactgatg
     4561 ggtgtcggtt attgtacaat gggcatacat aatatcacca tgctctatgt atccatggcc
     4621 ctggtgatta tcggcaatgg atttttcaaa cccaatattt caacgctgct gggaaatgtt
     4681 tattccacgc ctgaacatct ccatcagaaa gatgaaggat ataatatctt ttacatggga
     4741 attaatgtag gcgcctttgt ctgtaacttt tttggagccg ctctttatac catgatcggc
     4801 tggggagccg catttgccgc agctggtgtc ggtatgttta tcggcgtgat cacctttatt
     4861 gcgggcaccc ggcattacaa agatttcgat gtacgcaaac ccctgaagcc gggcgatatg
     4921 ccgctcggaa aaattctggc gctagtgatc ctcccgtcga ttttatttgg ggtgatcggc
     4981 tggtttatca aaggcacaaa tggttatatt ttcgattcca attccaccga cgccttcatc
     5041 tttgcctgta tccctgtctg tgtattttat accgcattat tattacgcag cagtaaggat
     5101 gaacgtccgg ccattggcgc catgctcgct atttttgccg tggtaatttt attctgggcc
     5161 atctttaaac aaaacggatc tgcccttaat acatgggcaa gccggtatac ggatcgggaa
     5221 gtaacaggta ctacaggtga tttcctggcg gccttaaaac aaaccgacaa agccgccaac
     5281 accacgtata aactcgattc agtcaccaag ctggataatc agttccgcaa agtaagggac
     5341 gcccagggta atgaagtgaa agaactgggt tacccctctt attttaaaaa tgtagccccc
     5401 gaaaaactac ctgctgaagg ggcagtggtc aaagcctggc tgcccagctt gtcccaatcc
     5461 atcaatcctt tctgggtgat tgcccttacc ccgctggtgg tggctttctt tacctggctg
     5521 agaagaaaag gcaaagagcc ttccacgcct accaagattg cgtttggttt actggtctcc
     5581 gctttatccg tattagtaat ggtggcggct gtaaaagccg gccataacgg tacggaaaaa
     5641 gtcagtgtat ggtggctggt gggtagttac ggggtggtca cgattggcga acttttcctc
     5701 agtccgatgg gcttatcact ggtctccaaa ctcagtccgg ttcgtatcac ttctctgatg
     5761 atgggcggct ggtttttatc aacctctatc ggcaataaac tcagcggcat cctcgccagt
     5821 aaatgggatc agtatgaaga caagactgat tatttctggc tgaactttat attactgggc
     5881 ggcgcagcca tcttcctttt tgtactgctg aaatggctga accgggtgat gaaggagaag
     5941 ggagtgaact gatagttgtt ggtgataaca ccaacaacca tcagtccgct gttggtgttt
     6001 tcaccaacgg ccagtccgct gttggtgttt tcaccaacag tgatcagtct gctgttggag
     6061 ttctcaccaa cagcaagtat cttccatcta taaacctcta ctgcatatgt ctgaacagca
     6121 aaaagtattt cgcccctttg ttccaccgga agcaaggatg gccgagttca ccatcaaatc
     6181 tgtgatcact ggttctatct tcgggatcat attcggtgcg gcaaccgttt acctcgccct
     6241 taaagctggt cttactgtct ccgcctctat tcccatcgcc gtattatcca ttgcattggg
     6301 gaaattattt ctgaagacaa ccattcttga gaataatatt atccagacaa ctggtagtgc
     6361 cggagagagt attgcggcgg gtgttgtttt cacattaccc ggcttccttt ttctcagttc
     6421 gcctgacagc agcagctatt ttaattacct caccattatg accctggcca ttctgggcgg
     6481 tatgctgggt acattaatga tgatcccgtt gcgccgctcc ttaatcgtta aagaacatga
     6541 gacactgccc tatccggaag ggactgcttg tgcctctgtg ttgaaggctg gtgaaaaagg
     6601 aggtgacctg gccaagaccg cctttatggg aatgggcgta gccattatct acgctttcct
     6661 gcagaaagta tttcatgtaa ttgcagaagt accggtttat gctactcatc ttaaaaataa
     6721 atacctgccc tccgcaaaaa tcagcggtga gatcaccccc gaatatctcg gggtgggcta
     6781 tatcatcgga cctaaaatat ccggtatcct ggtggcaggt ggtgtgctca gttcctttgt
     6841 actgattcct ttgctggcta cgctggttcc cggagatgcc acttttgcac agttaaccaa
     6901 actcggtttg aatccggcca agttcggatg ggatgctacc tcacatactt ttactgattc
     6961 agcggaagcg ctgtacagag cctatatccg gcagatcggt gctggtgctg ttgcggcagg
     7021 tggttttatc accctgatta aaactatccc tactattata tcttccttca aagaaagcat
     7081 tggtgcggta aaagataaat cagcaggcaa aagtgtgatc cgtacggaac aagatctgag
     7141 tatcaaagtg gttggtctag gtagtcttgg cctgatcctg atcatggccc tgctctccat
     7201 tatcccggga gaaaatatcg gcagccgttt attattaggt gtattggtga tcatcttcgg
     7261 cgctttcttt gttaccgtat cttcccgtat cgtaggactg atcggttcct ccaataatcc
     7321 tatcagtggt atgactattg ccaccatcat ggccacctgc ctggtattta tcggggtagg
     7381 ctggagtggt aagctatatg agcccatggc actcgtggtc ggaggcatga tctgtgtggc
     7441 agcggctaat gccggcgcca cttcacagga tttaaaaacg ggttacctgg ttggggctac
     7501 gccaaagtac cagcagctcg ccttatttat cggtgccatt gtatcatcaa ttgtaatcgg
     7561 ctggacagta aaaatcctgg acactccatc tgctgcgctg gccgccactg gcgtacagca
     7621 tgctattggt gaaacctatt ccgcaccaca agccaccctg atggccacct tgattaaagg
     7681 aatcctgtcc ttcaacctcg actggcaatt tgtactcatc ggtgtattca ttgccatagt
     7741 actggaactc tgcggtgtga aagccctgag ttttgccgtt ggtacctacc tgcccttgtc
     7801 cactacattg cccatcttta ttggaggatt tataagagga gtggccgacc gaaaggctaa
     7861 aaaagacagt gaaccggagg aagaggagga cctgcgcaaa ggcaatctgt ttgcaacagg
     7921 tctggtggcc ggaggcgcct tgttcggggt cattgcggct atcctcactg tattctttga
     7981 atccgccatg aaatcggtaa acctcgaaga aggtgttacc ggcagtttcg gacatagtgg
     8041 ttatatggca ttgggcgttg gcttttttgc ggccatgaca tttatacttt ataaagtggc
     8101 aacggggaag aagaagtgag tggtgaatgg tgagtaggga gtgggcagtt gaactaaact
     8161 tgaaaaggca ttacagcttt gatattatat caaacctgta atgccttttc cggtgttacg
     8221 ccctactcac tactgactac tcaccactca ctatatgaaa ttcagatact catacttcct
     8281 ccccaaaatc cccacatcat ccgcctgcaa ccatttattc aggatcgtag catatacatt
     8341 cttaaagtcc acattatatt tcagatcgcc ttcattcagg ttcgccaggt caggcatggg
     8401 attcagtact cctttctttt tcagtccgcc actcaccagg aacatattat tggccgtacc
     8461 atgatcggtt ccgccgctgg cattctgttc cacccggcgg ccaaactccg agaaggtaaa
     8521 taataacaca tcctcaaagc ggtgttgttc cttcaggtct tttacaaacg attttacggc
     8581 gtcattcatt tccgtaaaca ggcgttgttg ctgcgcttcc tgattgatat gggtatcaaa
     8641 actacccagg gagacatagt agaccttggt attgatctct gaaaaaatca gggaggcaat
     8701 cgtcttcagg ctgttgccaa ggtcggtctt cggatagtcg gcattgctgg gatgcagccg
     8761 gctttgttga aaaatataat cggcactgga gagggtttcc gccatggttt tatagagata
     8821 gtccacaggt tgctcaccgg cttcatcttt atggttcttc atcacttccc ggaaaaactt
     8881 ttcattcgct gtaccatata aacgacgggg atcttttacg gcgattcctt ttatattttc
     8941 tcccttaagg gcaagactca gcacatcatc cagttcaatg gcctgcgtgg gtttatcaca
     9001 acctttacac tgtgcatcca ggtaacgccc cacccaaccc tgactccagt actccgtgct
     9061 ctgactggcc gtatgccaga tatccatact ccggaaatgg gaccggtcgg gattgggata
     9121 tccgacatta ttcaaaatgg acagactgcc atcatcatac aattccttaa aagccgtgag
     9181 ggccgggtgt aaccccgttt catcggtaag acttaatgct ttatcccgga gaattcccag
     9241 gcgtggtctt gctttataat acaggtcatt ccggtaagga atcacggtat tcaatccgtc
     9301 atttcccccg ctcagttgca gtaccaccac cactttattg ccgggcatca cacgggccgg
     9361 actttcaaaa gccttcagaa acttgggcac catcagggaa gcagtggcca gggaaccgat
     9421 ttggatgaac ttctttcttt tgatatacat aaataaagat taaagacttt ttgatgatat
     9481 tctttaagct acgagtggcg agctgtgagc tacgagccct tcaatagtaa aattcccaac
     9541 ggcaggcaga cacaaaatct cgcaactcgt aactcgctgc tcgaagcttt tcttaacaaa
     9601 gttgatactc aggagtactc atcacctgta tagtcgctgt tttaataaaa ttctcccttc
     9661 cggtctgatc cgaaaatcgg gtgatcagtt cttcactcac cgatgatttt gtctgcagta
     9721 aaaaactgct ga
//
LOCUS       DAOPMW010000089         9731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7611_length_9731_cov_9.611720, whole genome shotgun sequence.
ACCESSION   DAOPMW010000089 DAOPMW010000000
VERSION     DAOPMW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_9731_cov_9.611720"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..967
                     /locus_tag="PLU11_06220"
     CDS             557..967
                     /locus_tag="PLU11_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HPN58746.1"
                     /translation="MLYSNNDYRKYLQPYANKLRKNMTRAEACLWKYALKSKKLKGYT
                     FRRQRPVLKYIADFMCLDLKLVIEVDGGTHLEAATIAKDKVKQKDLEEAGYTVLRFTD
                     HEVLKHMNAVIGSIEEWVEQYEKSKSAERGQSGK"
     gene            complement(1088..2101)
                     /locus_tag="PLU11_06225"
     CDS             complement(1088..2101)
                     /locus_tag="PLU11_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58747.1"
                     /translation="MIQRILLLLALSFSLVTSAQPEEVKKMHETAKAFMRTGDFDNAI
                     IVLTRALQKDSKNLELQKDLVMSYYLKRDYSKAMEGAKELISRDDADVVSFQIAGNVY
                     KALEEVKDCDKMYKKALKKFPKSGPLYSEYGELLWSAKDMLAIRQWEEGIKNDPGYSG
                     NYYNAALFYFYTKDKVWSLIYGEIFVNMESLSERGASMKGLLLQGYKEKLFADADILK
                     GEEKNKSEFAKAFLQGIGKQSSLINNGINTETLTMIRSRFILDWYETNAAKYPFKLFD
                     FQRQLLQEGMFDAYNQWLFGTSENLAAYDNWTKAHAEEYEAFSKFQKSRIFKMPAGQY
                     YQN"
     gene            complement(2179..3399)
                     /locus_tag="PLU11_06230"
     CDS             complement(2179..3399)
                     /locus_tag="PLU11_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPN58748.1"
                     /translation="MAVIKPFNALRPQQQLSQQVASRPYDVLNSEEARTEAAGNPFSF
                     LHITKSEIDLPPGIDIHSQDVYDKAKENLNQFIGNGIVFEEEHPCYYIYQLVMKGRSQ
                     TGLVCVSSVDDYFKDVIKKHEFTRPEKEKDRIDHMRTIRAQTGNVFLAYRDVDAVNRL
                     IHDWQSDHQPVYNFTAADGIQHAIWVVNEQSAIDAITTEFAQNVPCTYIADGHHRAAS
                     AAKVSKALPDSTDAGYFLTTIFPASQLAILDYNRVVKDLNGLTPAKFLGSLQDDFYIT
                     HSPEAVKPSQLHEFGLYLDGKWYILSSRKGTWTEDPIGVLDVTILSHNILDKILDIKD
                     QRTDKRIDFVGGIRGLKELEKRVDSGEMKAAISLYPVTIEQLFTIADSGNVMPPKSTW
                     FEPKLRDGLLTHLI"
     gene            complement(3405..4484)
                     /gene="serC"
                     /locus_tag="PLU11_06235"
     CDS             complement(3405..4484)
                     /gene="serC"
                     /locus_tag="PLU11_06235"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPN58749.1"
                     /translation="MKPVIHNFNAGPSVLPREVFEEASKAILNFNDTGLSILEIGHRT
                     SLFQPVMDEARSLVKELMQLGDKHEVLFLHGGATTQFMQVPMNLLNENDIASYTETGT
                     WAGKAIKEAKLYGHVEIAGSTKTDNYCSIPDELSVSPTAAYLHITTNETIAGTQWQAI
                     PYDCGVPLVADMSSDILSRQLDFNRFDLIYAGAQKNMGAAGVNLVVVNKEILGKVDRT
                     IPTILDYRNHISEGSMLNTPPVFAVYVVLLTLRWLKAKGGVAAIEKLNDEKATLFYNT
                     LDSLPVFRGPVAKQDRSRMNAVFVMENPALENEFMELCKKENMIGIKGHRSVGGFRVS
                     MYNALTIDSVKALTDLMKDFATKKG"
     gene            complement(4533..5501)
                     /locus_tag="PLU11_06240"
     CDS             complement(4533..5501)
                     /locus_tag="PLU11_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HPN58750.1"
                     /translation="MKKTSLLLLLIICSLPVFSWGFYGHRMINYYAVFLLPPEMMLLY
                     KPNIDFLTEHAVDPDKRRYTVPEEGPRHYIDLDKYGQYPYDSLPRNWNEAVKKYTADS
                     LQKHGIVPWWVQTMLFRLTQAFKDKDHVKILKLSAEIGHYISDAHVPLHASSNHNGQY
                     TNQRGIHGFWESRVPELLAEKHWDFFIGKAEMISNPSGFIWARVLESAAAADTVLRFE
                     KELSRQFPPDQKYAFEDRNGTVIRQYSSAYTVRYNELLKGMVERRMRQSIYAVASFWY
                     TAWVNAGQPDLRALSNREFTSADQEAFEQLNRDWKNNAIKGREHDH"
     gene            complement(5655..6266)
                     /locus_tag="PLU11_06245"
     CDS             complement(5655..6266)
                     /locus_tag="PLU11_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPN58751.1"
                     /translation="MAAPKPSSPKVLRKILRYFIQGIIILAPIGITLYALYWLFQKVD
                     GILRPFVNIPGLGFILIIIFVILVGWISSNFLMGSAITFFDQLMERTPGIKFIYSSTK
                     DFFEAFAGDKRKFNRSVLANVFSADVWIVGFLTDEEMEKFELGADKVAVYVPQAYNFA
                     GQLYILPREKVRKIEKISAGEAMKYAVTGGVVDFEEEKNKEEA"
     gene            6407..7045
                     /locus_tag="PLU11_06250"
     CDS             6407..7045
                     /locus_tag="PLU11_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN58752.1"
                     /translation="MEPKERISAKAEELFMQFGIRSVSMDDIAHSLGMSKKTLYQYYT
                     DKDELVYAMIESHIRDMQQDCESCREQANDAIHEIFITMERIVDELSNMNPMVLYDLE
                     KFHHKAFQHFKEHKDKYLVKIVRNNIEWGISQGLYRSELNVDVMSKYRMESMMIPFNV
                     AVFPPGKYNLATISEMIIENFIYGLTTIKGHQLIQQYYEQRKNHLTYEANRE"
     gene            7026..8459
                     /locus_tag="PLU11_06255"
     CDS             7026..8459
                     /locus_tag="PLU11_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN58753.1"
                     /translation="MKRTGNKRGLLLAVLLPLLGAAQSGGGTNPDTTKPTKHEFTIQQ
                     AVDYAKKNNLNVKNALLDVQYQEQVNKEITSRAYPGINASLSTTYNPNVATQVLPNFI
                     SPSTYQVLIDQGVKDGNGNPIQMPADFGFIAAQFGTKYSASAGIALSQLLFDGQVFVG
                     LQARDATMRFARKNVEVTEEMIKTNIYKIYYQLVVSKTQVELLEANIQRLEKLQHDTK
                     IIFENGFAEKLDVDKLNVALTNLRTEKGKVMNSISNGYYGLKVLMGMPVKDELVLTDL
                     LTEEQIKDGILEASIYKYEDRKEFQYAQIGKELNEFNIKRYKLSQIPTISLSGQYAKN
                     AQRNKWNFLGKGDWFTISSVNLNMSIPIFNGFYTKSKIQQTRIDLRKTENQIEALKLS
                     IDSEVETAKNNYRSAISTMDYQKKNMELAETVYQQTKKKYEVGTGSQTEINAAQVELK
                     TAQTSYITALYDAVIARIDFLKATGKL"
     gene            8495..9709
                     /locus_tag="PLU11_06260"
     CDS             8495..9709
                     /locus_tag="PLU11_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN58754.1"
                     /translation="MNTILKIATALLLTVSIAACGGSAKEEKGKLTDLKTELDKLKKD
                     KSGLDDQIKKIEEQIAKLDTNAASRVKTLVAVDTLRTSDFAHYIDLQGKISASGIGYV
                     APKGQGGLIRSIFVSVGQKVGVGQLVLKLDDAIQRQQLASAQQAVSQLKTRLAQAQTV
                     YERYQNLWKQNIGAEINVINAKADVDAMSAQLKSAEAGVGQAAEALDMTNVRAGMSGV
                     VEEVNVRVGEFFSGATQQGGQIVIVNAASLKAEVPVPDNYVAKVKKGDKVLIAVPETG
                     KAPYASVISVVGASIDATTRSFLTEARLPADPLLKPNQTALMKILDYEARGTIVIPVN
                     TVQSDENGKFVYVMEKAGDKMVARKKVVNVGEVYEGRIEIKSGLSGGELLITEGYQSV
                     YDGQSIATSANK"
BASE COUNT         2653 a         2120 c         2379 g         2579 t
ORIGIN      
        1 ttgtttttat gtaaacactt gaggtgtaac gtgggttcaa tatactattt ttttgaatag
       61 ttgctgtgag gaaggtgtca ggtgtgaggt gtcgggtgtc aggtaacagc cagctcgggg
      121 aaaaggtgtt cgctgttagc tgttaggtgt tgggaactgc cggggagagt agcgagtttt
      181 ggctttcctt cccgttctcc acgacttccc ggctctataa tctcttgccg aaggcaattc
      241 ctgcggaata ttttttagcc gggaagaaag gaagacggga agatatgagg ttgaatctag
      301 aaaaaacaag aagctactcg ttttacggca tgcctttcgt acttagaaag gctttacgcc
      361 agccggattt caggagggtt cattgtcccc cgctggcggg gggagggctt ccactttaat
      421 gaattttcat tttggggtgg atttggaagc acttttttaa atgttcacgt gaaatttcac
      481 ccggaaccgc agaaaaaaag cagtgctacc cgtttttttc acctacctaa gatctgtatc
      541 gctgctattt tagccaatgc tttattctaa taatgattac cggaaatacc tgcagcctta
      601 tgcaaataag cttcgcaaga atatgaccag ggcagaagcc tgtttgtgga aatatgcatt
      661 aaagagtaaa aagctgaagg gctatacttt caggaggcag cgaccagtgc taaagtatat
      721 tgctgacttt atgtgcttag atctgaaatt ggtcattgag gtagatggtg gtacacatct
      781 ggaggcggct actattgcta aagataaagt gaaacaaaaa gacctggaag aagcaggcta
      841 caccgttctg cgatttactg atcatgaagt gttgaagcat atgaatgctg tgatcggttc
      901 tattgaagaa tgggtggagc agtatgagaa aagcaagagt gctgaaagag ggcaatcggg
      961 gaaataggtt tttgaagaga tatctctaaa gtccaccccc atttgaagag gctttaatac
     1021 tgactggcgt acccccgcca gcgggggaca gggaacagtc gtccagttcg attggcgtac
     1081 ctcgtactta attctggtag tactgtcccg ccggcatttt aaagattctt gacttctgaa
     1141 acttgctgaa tgcttcgtac tcttccgcat gcgcctttgt ccagttgtca taggcggcga
     1201 ggttttctga agtgccgaag agccattgat tgtaggcatc gaacatacct tcctgcagga
     1261 gctgacgctg gaagtcgaat aatttaaacg ggtatttagc ggcattggtt tcgtaccagt
     1321 caagaatgaa gcgggagcgg atcatcgtca gcgtttcggt attgattccg ttattgatca
     1381 gggacgactg tttgccgata ccctgcagaa aggctttggc gaattcactt ttattcttct
     1441 cttcaccttt caggatatcc gcatccgcaa ataatttttc cttataaccc tgcaacaaca
     1501 atcctttcat ggaggcaccg cgctcgctca gactttccat attgacaaag atttccccgt
     1561 agatcaggct ccagactttg tctttggtat aaaaataaaa cagggcagca ttgtagtaat
     1621 ttccgctata tccaggatcg ttttttattc cttcttccca ctgacggatg gccagcatgt
     1681 ctttggccga ccacagcagt tcgccatact cgctgtagag cgggccgctt ttggggaatt
     1741 ttttcagtgc tttcttatac atcttatcgc aatccttcac ttcttccagg gccttgtaca
     1801 cattcccggc aatctggaag ctcaccacat ccgcatcatc ccggctgatt agttcttttg
     1861 ccccttccat ggctttactg taatcccgct tcaggtaata actcattacg agatcctttt
     1921 gcagttcaag gtttttacta tccttctgca gtgcccgggt caggacgata atggcattgt
     1981 caaaatcacc ggtgcgcata aaggctttgg cggtttcatg cattttcttc acttcctccg
     2041 gttgggcgga agtaaccagg gaaaaggata aagcaagcag gagtaaaatt cgttggatca
     2101 tggtgcaaat taaacaaaca gagacgtact aaccgacgtc tctgttgcaa aataacaaat
     2161 atttcagcgt ttaaccggtc agatcaggtg ggtcagcagc ccgtctctca gttttggttc
     2221 aaaccaggta ctcttgggag gcattacatt gccactgtcg gcaatggtga atagttgttc
     2281 gatcgtaacc gggtacagac tgatagcggc tttcatttca ccactatcca ctcttttttc
     2341 cagttctttc aacccacgga tccctccaac aaaatcaatc cgtttatcag tacgctggtc
     2401 ttttatatcc agaatcttat caagtatatt atgcgaaagg atcgttacat ccagcacccc
     2461 gatcggatct tcggtccagg taccttttcg tgagctcagg atataccatt tgccatccag
     2521 gtataggcca aattcgtgta attgggaagg cttaacggct tccggactat gcgtgatata
     2581 aaaatcatcc tgcagtgatc cgagaaattt ggccggggta agaccattga gatctttgac
     2641 cacgcggtta taatccagga tggccagttg actggctgga aaaattgtag tcagaaaata
     2701 accggcatcg gtactgtctg gaagtgcctt gcttactttg gctgcagatg cagcgcgatg
     2761 gtgtccatcg gcaatatagg tgcatggaac gttttgcgca aactctgttg taatagcatc
     2821 gatcgcggat tgttcattta ccacccagat ggcatgttgt atcccatcag cagcggtgaa
     2881 attatatacc ggctgatgat ccgattgcca gtcgtggatc aggcggttta ccgcgtccac
     2941 atccctgtaa gcgaggaata catttccggt ctgagcccgg atggtacgca tatgatcgat
     3001 ccggtctttc tctttctccg gacgggtgaa ctcgtgtttt ttaatcacgt ctttgaaata
     3061 atcatctacg gaggacacac ataccaggcc ggtctggctg cggcccttca tgaccagctg
     3121 gtagatatag tagcagggat gttcttcttc aaatacaatt ccgttaccga taaactggtt
     3181 gagattttct ttggccttat cgtatacatc ctggctatgg atatcaatcc ccgggggcag
     3241 atcaatctct gatttggtga tatgcagaaa agagaacggg ttgcctgctg cttcggtgcg
     3301 tgcttcttca ctgttcagta catcataagg acgggaagcg acctgttgtg ataattgttg
     3361 ctgcggtctt aatgcgttaa atggtttgat aacggccatg gggactatcc tttttttgtt
     3421 gcgaaatctt tcatgaggtc ggtcagtgct ttcacactgt cgatggtcag tgcattatac
     3481 atcgacaccc ggaagccacc cacactgcgg tgccctttga tgccgatcat attttctttt
     3541 ttgcacagtt ccataaattc attttcgagt gccgggtttt ccatcacaaa taccgcgttc
     3601 atccggctgc ggtcctgttt ggcaaccggt ccacgaaaca ccggtaaaga atcgagtgta
     3661 ttatagaaaa gtgttgcttt ttcatcattc agtttttcaa tagcagccac tccgcctttt
     3721 gctttcagcc agcggagcgt gaggagtacc acatacacag caaaaaccgg cggggtattc
     3781 agcatactgc cttctgaaat atgattgcgg tagtcgagta tggttgggat ggtacgatcc
     3841 acttttccga gaatttcttt attcaccacg accaggttta caccggcagc acccatattt
     3901 ttctgggctc cggcatagat caggtcgaag cggttaaaat ccagctggcg actgaggata
     3961 tcactgctca tatccgctac cagtggtacg ccgcaatcat aaggaatcgc ctgccactgg
     4021 gtgccggcaa tggtttcatt ggtggtgata tgcaggtaag cggcggtagg ggaaacgctc
     4081 agttcatccg gtatactgca ataattgtct gttttagtgg aacccgcaat ttcgacgtgt
     4141 ccgtaaagct tggcttcctt gatagcttta cccgcccagg tacctgtttc ggtataggaa
     4201 gcaatatcat tttcattcag caggttcatc ggcacctgca tgaattgtgt ggtagcacca
     4261 ccatgcagga agagtacttc gtgtttatcg cccagctgca ttaattcttt taccagggat
     4321 cttgcttcat ccatgacggg ttgaaacagg gaagtgcggt gaccgatctc gaggatcgat
     4381 aatccggtat cattaaaatt cagaatggct ttactggctt cttcaaaaac ttcgcggggt
     4441 aatacagagg gtcctgcatt gaaattatgg ataactggct tcacggttac agcggtaata
     4501 aatgagttgc aaatgtaggg aaattgcccc ccttagtggt catgttcccg gcctttaatc
     4561 gcattgtttt tccagtcgcg gttgagctgc tcaaaagctt cctggtcagc cgatgtaaac
     4621 tccctgttac tgagggcacg gagatccggc tggccggcat tgacccaggc cgtataccag
     4681 aaggaagcca ccgcgtaaat ggactgacgc attcttcgtt ccaccattcc tttcagtaac
     4741 tcattgtatc tgaccgtata ggcactggaa tactgacgaa tgaccgttcc gttgcggtct
     4801 tcaaatgcgt atttctgatc gggtggaaat tgccgtgaca gctctttttc aaaccgtagt
     4861 accgtatcag ctgcggctgc actttcgagt acgcgtgccc agatgaagcc tgatgggttg
     4921 ctgatcattt cagcttttcc gataaagaaa tcccagtgtt tttcagccag cagttcggga
     4981 acccggcttt cccagaagcc atgaatgccc cgttgattgg tgtattgtcc gttatgattg
     5041 ctggacgcat gcaggggtac atgggcatcg ctgatatagt gaccgatttc tgcagagagt
     5101 tttagaattt ttacatggtc tttgtcttta aaagcctggg tcagccgaaa gagcatggtc
     5161 tgtacccacc agggaacaat gccatgtttt tgcaggctgt cggccgtgta tttttttacg
     5221 gcttcattcc agttacgggg caatgaatca taggggtatt gaccatattt atcgaggtcg
     5281 atataatgac gggggccttc ttcgggtacg gtatagcgtc gtttgtccgg atccacggca
     5341 tgttcggtca ggaaatcgat attgggctta taaaggagca tcatttccgg aggcaggagg
     5401 aaaacggcat agtaatttat catccggtga ccataaaagc cccagctgaa aacagggagg
     5461 gaacaaatga tcagtaacag gagaagggaa gtttttttca tggaatagtt ttcccaaaac
     5521 tatgttgcag gtaacaggca tccatgggta aaacaacaag gcttgctgtc tttttcctga
     5581 ttttagtagc tgggaaaagc gggaaaactt cagggaagcg aaatagcaga tggtaagcaa
     5641 aggggtggga tgaactaagc ctcttcttta ttcttttctt cttcgaaatc cacaacccca
     5701 cccgtaacgg catatttcat tgcttcgccc gctgaaattt tttcaatctt tctgactttt
     5761 tcgcggggta aaatatagag ctgaccagca aagttgtagg cctgcggtac atataccgcc
     5821 actttatcag ccccgagttc aaatttttcc atttcttcat cggtcaggaa gcctactatc
     5881 cagacatctg ctgaaaagac attggccagt acagaccggt tgaatttccg tttatctccc
     5941 gcaaaggctt cgaagaaatc cttggtggaa ctgtaaatga atttgattcc aggcgtccgc
     6001 tccatcaatt ggtcaaaaaa ggtgatggca cttcccatca ggaaattgga gctgatccaa
     6061 cccacgagga tcacaaagat gataatgagg ataaaaccaa gccccgggat atttacaaaa
     6121 ggccttaaaa tgccgtctac tttctgaaat agccaatata gtgcgtataa agtgatccca
     6181 atcggagcta aaatgataat tccctgtata aaatacctta atatttttct taatacttta
     6241 ggagaggatg gttttggcgc tgccattttg cggaattata tagctaaggt agggtaaaaa
     6301 ggagcgcgtt gaccggtcaa aataatcggt gaacggtcga aaaagcagga tggaaacttt
     6361 tgttacgaaa atagtttccg tagttttgcc ctctcaattc aacaaaatgg aacccaaaga
     6421 acgaatctcc gccaaagcag aagaactgtt tatgcagttt ggcatccgtt cggtatccat
     6481 ggacgatatt gcccatagcc tgggaatgag taaaaagacg ctttaccaat actatacgga
     6541 taaagatgag ctggtatacg cgatgattga aagccatatc agggacatgc agcaggattg
     6601 tgagagctgc cgggaacagg ccaatgacgc gattcatgaa atcttcatta cgatggaaag
     6661 aattgtggat gagctgagca atatgaatcc catggtgctg tacgatctgg aaaaatttca
     6721 ccataaagcc ttccagcatt tcaaagaaca taaagacaaa tacctggtaa aaattgtacg
     6781 caacaatatt gagtggggga tttcgcaggg actctaccgg agcgagttaa atgtggacgt
     6841 catgtccaaa taccgaatgg aatcgatgat gatccctttc aatgtggcgg tctttccccc
     6901 gggaaaatat aacctggcca cgatttcaga aatgattata gaaaatttca tttacggact
     6961 gaccaccatt aaaggacatc aactgataca acaatattat gaacaacgca aaaaccatct
     7021 tacttatgaa gcgaacaggg aataaacgcg gactgctcct cgctgtcctg ctccccttgc
     7081 tgggagcggc tcagtcgggc ggaggaacaa atccggatac cacgaaacca acaaaacatg
     7141 aattcaccat tcagcaggcg gttgactatg caaagaagaa caacctgaat gtgaagaatg
     7201 ccttgctgga tgtacagtac caggagcagg taaataaaga aatcacctcc agggcttacc
     7261 cgggtatcaa cgccagtctg agtaccacct acaatcccaa tgtagctacc caggtgctgc
     7321 ccaactttat ttcgccttcc acttaccagg tgctgattga ccagggcgtg aaagatggca
     7381 atggcaatcc gatccagatg ccggcggatt ttggttttat agccgcccag tttggtacga
     7441 aatacagcgc cagtgcaggc attgcacttt cccagttgct ttttgacggc caggtattcg
     7501 taggactcca ggcccgtgat gccaccatgc gctttgcccg gaagaatgtg gaagtaacgg
     7561 aggaaatgat caaaaccaat atttataaga tctattacca gctggtggtg agtaaaaccc
     7621 aggtggaact gctggaagca aatattcagc ggctggaaaa actccagcat gatacaaaga
     7681 tcatttttga gaatggcttt gctgaaaagc ttgatgtgga caaactgaat gtggccctga
     7741 ccaacctgcg gacggagaaa gggaaagtga tgaacagtat ttccaacggt tactacggtc
     7801 ttaaagtatt gatgggtatg cctgtgaagg atgaactggt gctgacggat ctgcttaccg
     7861 aagaacagat taaagacggc atccttgaag ccagtattta caagtatgaa gaccggaagg
     7921 aatttcagta tgcccagatt ggtaaggagc tgaatgagtt taacatcaaa cgttataaac
     7981 tgagccagat cccgactatt tccctgagcg gtcagtatgc gaagaatgcc cagcggaata
     8041 aatggaactt cctgggtaag ggcgactggt tcaccatttc ttcggttaac ctgaatatgt
     8101 ctattccgat ttttaacggc ttttatacca aatcaaaaat ccagcagaca cgtattgacc
     8161 tgcgcaaaac cgagaatcag atcgaagcgc tgaaattatc cattgacagt gaagtggaaa
     8221 cggcaaagaa taattaccgt tctgcgatca gcacgatgga ttaccagaaa aaaaacatgg
     8281 aactggcgga gactgtctac cagcagacca agaaaaagta tgaagtgggg accggttcac
     8341 aaaccgagat caacgcggca caggtggaac tgaaaacggc gcagaccagt tatatcaccg
     8401 ctttgtatga cgcggtcatt gcccgtatcg attttctcaa agccaccggc aaactttaat
     8461 aaaaaaccaa ccattgactt ataaattaaa aaatatgaat acgattttga aaattgctac
     8521 agcactactg ctgacagttt ctatagctgc ctgtgggggc agtgccaaag aagagaaagg
     8581 taaactcacg gatcttaaaa ccgaactgga caaactcaaa aaggataaat ccggattgga
     8641 tgatcagatc aaaaaaattg aagagcagat cgctaagctt gacaccaatg ccgccagccg
     8701 ggtaaagaca ctggttgcgg ttgacacttt acgtacttct gattttgccc actatatcga
     8761 tctgcaggga aaaatttccg caagcggtat tggttatgtg gcaccgaaag gacagggtgg
     8821 attgattcgt tccatctttg taagcgtagg ccagaaagta ggagtgggac aactagtcct
     8881 gaaactggat gatgccatac agcgccagca actggcttcg gcccaacagg ccgtgagtca
     8941 gctgaaaaca aggctggccc aggctcaaac tgtttatgag cgttaccaga atctctggaa
     9001 gcagaatatc ggtgcggaga tcaatgtaat caatgcgaaa gccgatgtgg atgctatgag
     9061 tgcccagctg aaatctgcag aagccggtgt aggccaggct gccgaagccc tggatatgac
     9121 gaatgtacgg gcaggaatga gcggagtagt ggaagaagtg aatgtacggg tgggcgaatt
     9181 tttctcagga gccacgcagc agggtgggca gattgtgatt gttaatgccg cttctttaaa
     9241 agcggaagtg ccagtgccgg ataattatgt agcgaaagtg aagaagggag ataaagtgct
     9301 gattgctgtt cctgaaacgg ggaaagcgcc ttatgcatcc gtcatcagtg tggtgggcgc
     9361 ctctatcgat gccacgacca gatctttcct gactgaagca cgcctgcctg ctgatccttt
     9421 attgaaaccc aatcagaccg cattgatgaa gatcctggat tatgaagcca ggggtacgat
     9481 tgtcattccg gtgaataccg tacagagtga tgagaacgga aaatttgttt atgtcatgga
     9541 aaaagccggt gataaaatgg tggcccgtaa aaaagtagtg aatgtaggtg aggtatatga
     9601 aggccggatc gagatcaaaa gcggactgag cggcggcgaa ctgctgatca ccgaaggtta
     9661 ccagtcggtg tacgatggac aatccatcgc caccagtgcc aacaaataat tacagccaaa
     9721 atactgatat a
//
LOCUS       DAOPMW010000090         9727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7615_length_9727_cov_8.765509, whole genome shotgun sequence.
ACCESSION   DAOPMW010000090 DAOPMW010000000
VERSION     DAOPMW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7615_length_9727_cov_8.765509"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /gene="corA"
                     /locus_tag="PLU11_06265"
     CDS             <1..1053
                     /gene="corA"
                     /locus_tag="PLU11_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPN58755.1"
                     /translation="FATKRTGEILSVNPTVISQRVDATEVSVSVYDYTAESLEEKKSD
                     KVESVFHYKDTPSISWINIDGIRKSDVEAACTNYGIHPLIIEDILSVDQRPKMDEVEG
                     VLYCLLNMLYFNEEKKTVETEQISMVLGSNFVISFQEDPSRDVFDPIRHRLKIPKSQL
                     RQRTADYLLYSMIDLVVDSYFLIMERLGEQIEALEEEVVRKSNKRSLARINQLRKELI
                     VLKRNIAPVRDLVAGIIRSESELLDDRTTKYFKDVYDHIIQAFDLSENYRDVMMSVQD
                     LYLSNVNLRLNEVMKVMTIVTCLLAPATVIGGIFGMNFDIIPYAHHSFGFYFAVGLMF
                     AIPVVMLYIFKKRGWF"
     assembly_gap    1086..1185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1391..4864
                     /locus_tag="PLU11_06270"
     CDS             1391..4864
                     /locus_tag="PLU11_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58756.1"
                     /translation="MMNASRRRLLKLLALSAGAGSARAISASPATIDQTLDALFRELA
                     DTVKLTLYRPEDLLELELVFTGYKKSPGNHSLQKTSGPQLLIVYFTPQALAEEAFEEG
                     GEAGSMSFSNNQTGPVKGNEKSAMGSQNPRFPARAYLSGRSRLVFSIPASVNSIPLTA
                     EALLNWDKFTPVVSKRAASPKLFPVFNASDTNDNYKNILIKEGPAINLNTPVTVPQGN
                     NQQPAVNNQNIRIQQLPANNADRQVQLNNERRAATKTEERQIQKTEAPITLTQTTVSP
                     QVIAIMDELRNGKTPRPVHPEETCIEFPYRLFISPNEYSAWNHDTKLKGRPIGGIAPG
                     IYYELWHTRLTCKNCVGGKDLSDSLKSIRSVRALWGTDINGDFKVKPARDNSFITSLY
                     NDDRHCIVHESSNWALPGGFVPKPVQVNNLMLSTLGAWFDAELEVKRNELDNAGLIGS
                     LNLLKWKHIATMARDHYVEVVYAGNMFPFGHEASLVRITERKPQAGYAVNRQRYFIVI
                     TEEEKKYDPYDPKTGSFRSFNFSTIKFISTSTPTIDPPKKFCNDLDNQADHQFMPMVG
                     GKNFQFKLVGYDLDGNETDFTMPLVFLTTDVTVKPGSNEINSSPITKVISCYNNSAFG
                     KNTAFRGQKLSLARSATPGDTLVEVQEIDFIGVLNVNEKPGFQPRVKQLDIYIEALEN
                     ITGNRKPVKVTLVDDEQHIQPNKRKNKGAVFARLVDASDVAFGGGKSKLKGGMMPDFS
                     VTGLSKTFGAVGGKLDDIMNTKFDPKSFFDDSAKLFGTIALGDIINVVDKVSTSMDGP
                     SLKTALPALKNYKTDQAFVTEYSWNAATLKAHDFGFGKFKPKNAAAGNVVIEAKLQRF
                     KDTSKGMLFELDSHIGAFDIILMDIAAVQFKRVGFKVNSSAKLDFSIDLEKNPIRFLG
                     ALSFVNDLQKFIPADGFSDPPFLDVTPSGVTTGYTLGLPDVQLGIFTLRHITLGAAIR
                     LPFDGSPLSLRFNFCERQQPFTLTVSALGGGGFFAIEFDMKGLKSLEAALEFGAAVSL
                     NLGVASGAVSVMGGIYFKMEMEQNGKKIELSGYVRVNGALSVLGLITVSVEFLLSLNA
                     EMKSAANGESKVSRMWGEATLKVKIEILFFSKTVKLKVQREFAGAGADPTFGMLISEN
                     EWLQYCDTFAA"
     gene            5032..9327
                     /locus_tag="PLU11_06275"
     CDS             5032..9327
                     /locus_tag="PLU11_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58757.1"
                     /translation="MPQKFIFTTSPWKDQGRNKWMLSLFISIQLDAGQRSQLSAFPDI
                     LSWMQKIADGDFFIQWDKNAPVAAEPLSAKWDPELYSRLFHGEIQVKTFTVPDVSKFI
                     IKSYPALHIRNYILDTYKEVGNLKMDELPTASFYTKQFTKLNEVSTVQLKNMKPLTGT
                     TRKAVINDFVVQGNTSVKNAQDQLRNKKTIGFNATASPKADFGQFHNFHQRVDMDKLQ
                     MKPIRKPEFEYHDMLSILTSYPVIMRKLGLVIDFELNAAPPAAAGTVRILPANLGLES
                     ESTLSSPATAYQYTGKGFYAAAKAGSQIDKGYLSIGGAGYEVVQIDTDGAAMKLSSHV
                     DTVNLNLGKRLVDKSNYIRPLNKFSRNKNVMLFNAGKISNLNAAPPADDDDDDDDPEE
                     GLPSLRSAGIGILKNGLAETILQKFIRSVDLTKITFASTAVLNNQALLNTAVSKTQVT
                     PTAPTTQPAVPNPPTKKTGGIAPIRMKRQIPAVRMQALTAAFQKIEYIPVPTEFLYAD
                     DLVFGYRLDIAYDDKPDTWYSLHKRKMSFAFAPVDQPVQPITLEPGDELDEGCIHLAL
                     TEDEEDGDEKKVNEVIARWEGWSLSVPRIGKGINNAGPEVSSDEDEKKKYKLDKETPF
                     RLQVEVKPAPKSLPKLRFGKKYRIKVRTVDIAGNGVSHDVQPESPSQTIRSGIEYRRF
                     EPLSSPVLLQGDEITGGDKKMIRDRDGESLQHMVIRSNAGVDTKTYEDKNITTVVVDG
                     SAKGTLKYHHEAVRFITAPRGSQQLAEIHGMFDEAMSDPQKAMQAYQFITSRDKEYKD
                     DGKTKAVQIPVETGQVDIDYLADPLAGGVVFTMRSNTSFETPWKKGQSRKFSFHFDDE
                     VSENDPGKKYSIEQWKNPRSVKIRLVEGNGEPEWKNRVFTIPLPKASIVEISYASFWA
                     PEALERFSGLMPRLQAGANGVKVKETANKGLHWMFSPWRKIRLVHAVQQPLEAPVMAK
                     ISKVERDYNDTAAQLLTLIKVHGASTEKMDLGAKWTEWVDDLSKPEPFNQAFSTHVET
                     VPVVYNDNMLELIKTPATKNPKPDWLPPITHLFGDTKYRHVGYIPSATTRFREYFTGI
                     IDTGRIKGEPLPLSREGEVVELDILSTARPTVPVLEYVVPGFVWSKSPGRGRNKVNMR
                     SGTIRVYLRRPWYSSGDDERIAVILPPEGLEPERHPEYKKYCTVWGMDPVFETERLPN
                     SFSNYPRKNHFVGTDVIYDTVKLAEENVPVQVAAYKVFFDKEKQLHYADIRVDAGNAY
                     FPFVRLMLARYQQHSLRENGTDVCLSQSVQADWIQQVPLRQVSFEELAGSFRIQLQGP
                     SAFRSGGRVRINITVEKALVPKTDNAFIGVNVPGEQSVVFTRDYNLDEKQLNAGKINF
                     EETVRVTGAGNQYRVVVREYELHPNDPLLAKPNIQGKNADTVGERLVFMEVFE"
BASE COUNT         2600 a         2342 c         2441 g         2244 t
ORIGIN      
        1 tttgccacca aaaggaccgg cgagatcctg tcggtgaatc ctacggttat ttcccaacgg
       61 gtggatgcaa cagaagtgtc ggtgtctgta tatgactata cggctgaaag ccttgaagag
      121 aagaaatcgg ataaagtaga gtcagtcttt cattataaag acaccccctc tatcagctgg
      181 atcaatattg acggaatccg gaaatcggat gtggaagccg cctgtacgaa ttacgggatt
      241 caccccctga ttattgaaga tatcctgagt gtggaccagc ggccgaaaat ggatgaagtg
      301 gaaggcgtgc tctactgcct gctgaatatg ctttacttca atgaagaaaa gaaaaccgtg
      361 gagaccgaac agatcagcat ggtgctcggc tccaattttg tcatcagttt ccaggaagac
      421 ccctcccgtg atgtatttga tccgatccgc caccggttaa agattcctaa aagtcagttg
      481 cggcaacgaa cagcggacta tctgctgtat tccatgattg acctcgtggt ggacagttat
      541 ttcctgatca tggagcgatt gggagaacaa attgaagcat tggaagaaga agtggtccgc
      601 aaaagtaata aacgttccct cgcccgtatc aaccagctgc gcaaggaact catcgtactt
      661 aaaagaaata tcgccccggt tcgcgacctg gtagccggca tcatccgcag cgaaagtgaa
      721 ctgctcgatg accgtaccac caaatacttt aaagacgtct acgaccatat catccaggcc
      781 ttcgacctca gtgagaacta ccgtgatgta atgatgagtg tgcaggacct ctaccttagt
      841 aatgtaaacc tccgcctcaa tgaagtcatg aaggtaatga ccatcgtgac ctgtctcctc
      901 gcccccgcca ccgtgatcgg tggtatcttc gggatgaact tcgacattat cccttatgcc
      961 caccacagtt ttggcttcta ctttgcggta ggactcatgt tcgccatccc ggtggttatg
     1021 ctttatattt ttaagaagag agggtggttc tgagtttttc ttcgtcaccc ttcgatacgt
     1081 cccgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaag aagagagggt
     1201 ggttctgagt ttttcttcgt cacccttcga tacgtcccgc acaagattca tttcagggca
     1261 agtgaaaaag tcgtgcggga cgtatcgaag ggtgcccgct aaagaacaat aagataaaaa
     1321 tcatacgccc catccctcta cattctgttt atttatttta catttaacat gaaacccatt
     1381 aacccatttt atgatgaatg cttcccgcag gcgcctgctg aagctgcttg ccctctctgc
     1441 aggtgcgggc tctgcccgtg ctatctccgc ttcgcccgcc accattgatc agacactcga
     1501 tgcccttttt cgtgaactgg ccgatacggt taaactcacc ctttaccggc cggaagacct
     1561 gctggaactg gaactggttt ttacggggta caaaaaatcc ccgggcaatc attccctgca
     1621 aaaaacctca ggtccgcaac tgctgatcgt gtacttcacc ccacaggccc tggctgaaga
     1681 agcgtttgaa gaaggcggtg aagccggctc catgagtttt tccaataacc agaccggccc
     1741 cgttaaaggc aacgaaaaaa gcgcgatggg atcccagaat ccccgcttcc cggcaagagc
     1801 ttatctctcc ggccgctcac gactggtctt ttcaattccc gcttctgtaa acagtatccc
     1861 gctgacagca gaggccctgc tcaactggga taaattcacc cccgtagtaa gcaaacgcgc
     1921 cgcatcacca aaactcttcc cggtttttaa tgcgtcagat actaatgata attataagaa
     1981 catcctgatc aaagaaggac cggcgattaa tctgaacaca ccggttacag ttccacaggg
     2041 aaataatcag caaccagcag ttaataacca gaacatccgc atccagcaac tgccggccaa
     2101 taatgcagac cggcaggtac aactgaataa tgaacgcagg gctgccacca aaacggaaga
     2161 aagacagatt cagaaaacag aggcaccaat caccctcacg cagacaacag taagcccgca
     2221 ggtgattgcg atcatggacg aactccggaa tgggaaaaca ccacggcccg ttcaccccga
     2281 ggaaacctgt atagaatttc cttaccgctt atttatttct cccaatgagt acagtgcctg
     2341 gaatcatgat acaaaactga aaggccgtcc cataggtgga atcgctcccg gcatctatta
     2401 tgaactctgg catacccgcc ttacttgtaa aaactgtgtg ggtggcaaag acctgtcgga
     2461 ttcgctgaag tccatccgct ccgtgcgggc gttgtggggt accgatatca atggcgattt
     2521 taaagtgaaa cccgcccgag ataattcttt tattacttca ttatacaatg acgaccgcca
     2581 ttgcatcgta cacgaaagca gcaactgggc cctgcccggt ggctttgttc ccaaaccggt
     2641 gcaggtaaac aacctcatgc tgagtaccct cggtgcctgg tttgatgcag agctggaagt
     2701 caaacgaaat gaactggata atgcgggact catcggcagt ctcaacctcc tgaaatggaa
     2761 acatattgcc accatggccc gcgatcatta tgtggaagtg gtgtatgccg ggaatatgtt
     2821 ccccttcgga catgaagcct cgctcgtccg catcaccgaa cgaaaaccac aggccggtta
     2881 tgccgtcaac cgccagcgtt attttatcgt gatcacggag gaagaaaaaa aatatgatcc
     2941 gtatgatcca aagacgggca gcttccgctc tttcaacttc tccacgatta aatttatcag
     3001 tacatccact cctaccattg atccgcccaa aaaattctgc aatgacctgg acaaccaggc
     3061 cgatcatcag tttatgccca tggtgggcgg taagaatttt cagtttaaac tcgtgggcta
     3121 tgacctcgat ggcaatgaaa ccgatttcac gatgccgctg gttttcctca ccacggatgt
     3181 aaccgtaaaa cccggcagta atgaaatcaa tagcagtccc attaccaaag taatcagctg
     3241 ttataataat tcggccttcg gaaagaatac cgcattccgc ggacaaaaat tatcactggc
     3301 ccgcagcgcc actcccggcg ataccctggt ggaagtacag gagatcgact ttatcggcgt
     3361 gctgaatgtg aatgaaaaac ccgggttcca gccacgggtt aaacaacttg atatttatat
     3421 agaagccctg gaaaatatta ccggaaaccg caagccggtg aaagtgacgc tggtggatga
     3481 tgaacagcat attcagccaa acaagcggaa gaataaagga gctgtatttg ccaggctggt
     3541 ggatgcatcc gatgtggcat tcggtggcgg caaaagcaaa ctcaaggggg gtatgatgcc
     3601 cgacttctcc gttaccggcc tttccaaaac ctttggagct gtgggcggca agctggatga
     3661 tatcatgaac acgaaatttg acccgaagag tttctttgat gattcggcca agctctttgg
     3721 cactattgca ctcggggata ttataaatgt ggtggataaa gtcagtacca gcatggatgg
     3781 cccttcattg aaaaccgccc tgcccgcctt gaagaattat aaaacggatc aggcctttgt
     3841 aactgaatac agctggaatg cggccacgtt aaaagcgcat gattttggtt ttgggaaatt
     3901 caaacctaaa aatgcagctg ccggcaatgt ggtgatcgaa gcaaaactgc agcgcttcaa
     3961 ggatacgtcc aaaggcatgc tctttgaact cgattcgcat atcggggctt ttgatattat
     4021 cctgatggat attgccgctg tgcagtttaa acgggtaggc tttaaagtta attcatcagc
     4081 caaactggac ttctccattg acctggagaa aaatccgatc cgtttcctcg gagctttgtc
     4141 ttttgtgaat gacctgcaga agttcattcc ggccgatgga ttcagtgatc cgcctttcct
     4201 ggatgtgacg ccttcgggcg ttacaaccgg ttatacactg ggcttacccg atgtgcaact
     4261 gggcatcttc acgctgcggc atatcaccct gggtgcggcc atccggttgc cctttgatgg
     4321 cagtccgctt tccctgcgtt tcaatttctg cgaacgtcag cagccgttta cattgactgt
     4381 atccgcattg ggcgggggtg gcttctttgc cattgagttt gatatgaaag gactcaaatc
     4441 gctggaagcg gcgctcgaat ttggggccgc tgtatcactc aacttaggag tagccagcgg
     4501 agcggtgagt gtgatgggtg gtatctattt caaaatggaa atggaacaga atggcaagaa
     4561 gatcgagctt tcaggatatg tgcgggtgaa cggggccttg tctgtgctcg gacttatcac
     4621 cgtatccgtt gagttcctgt tgtcgctgaa tgcggaaatg aaatccgctg ccaatggcga
     4681 atccaaagtg agccggatgt ggggagaagc aacattgaaa gtgaagattg aaattctgtt
     4741 cttctccaaa acggttaaac tgaaagtgca acgcgaattc gccggtgccg gtgccgatcc
     4801 gaccttcggt atgctcatca gcgaaaacga gtggctgcag tactgtgata cattcgcggc
     4861 ttaatcaccc ttcggcaagc tcagggtgac aacggccttt gcttgtacaa aaaacaccgg
     4921 tgtcatggtg agcctgccta ccggacaggc aggcttgccg aaccatgatg agcaaaaagt
     4981 agaacgaaaa cttatcaacc tttcaactta ttaacttatc aacccttctc catgccccaa
     5041 aaattcatct tcaccacgtc tccctggaaa gaccagggcc gcaacaaatg gatgctctcc
     5101 ctttttatca gcattcagct cgatgcaggg cagcgcagcc agttgtcggc cttccccgat
     5161 atactcagct ggatgcagaa aatcgcggat ggtgatttct ttatacagtg ggataagaat
     5221 gcacccgtgg ccgccgaacc actcagcgcc aaatgggatc ctgaactgta cagccggctc
     5281 tttcatggcg agatccaggt gaaaacgttt acggtgccgg atgtgtcgaa gtttattatt
     5341 aaatcttacc ctgcattaca tatccggaat tatatactgg atacctataa agaggtgggt
     5401 aacctgaaaa tggatgaatt gcccaccgca agcttttata ccaaacagtt taccaaactg
     5461 aatgaagtat caaccgtaca gctgaagaac atgaagccgc ttaccggcac tacccgaaag
     5521 gccgtgatca atgattttgt ggtacagggg aataccagtg taaagaacgc gcaggatcag
     5581 ctgcgcaata aaaagaccat tggtttcaat gccacggcca gcccgaaagc ggactttggc
     5641 cagtttcaca attttcatca gcgggtggat atggataaac tgcagatgaa accaatccgg
     5701 aagccggagt ttgagtacca tgatatgctg tccattctca cttcttatcc ggtgatcatg
     5761 cgcaaactcg gactggtgat tgattttgaa ttaaacgctg cgcctcctgc tgctgctggt
     5821 acggtgcgta ttctgcccgc caacctcggt cttgaaagtg aatcgaccct ttcgagcccg
     5881 gccactgcat atcagtatac aggcaaaggt ttttatgcgg cagccaaagc cggcagtcag
     5941 attgataaag ggtatttatc cattggggga gccggatatg aagtggtgca gattgatacc
     6001 gatggggccg ctatgaaatt gtccagccat gtggatacgg tgaacctgaa tctcggaaaa
     6061 cgcctggtag ataaaagtaa ttatatccgg ccgctgaata aattcagtcg caataaaaat
     6121 gtgatgctct tcaatgccgg caagatcagt aatctcaatg cggctccgcc tgcggatgat
     6181 gatgatgacg atgatgatcc ggaagaaggg ttgccttccc tgcgcagtgc cggtatcggt
     6241 atcctgaaaa acgggttggc cgaaaccatc ctgcaaaagt ttatccgcag tgtggatctc
     6301 acaaagatca cgtttgccag taccgctgtg ctgaataacc aggcgctgct gaacaccgct
     6361 gtttccaaaa cgcaggttac acccactgct cccaccactc agccggccgt tccgaatccg
     6421 ccaactaaaa agaccggcgg gatagccccc atccggatga aaagacagat acccgccgtg
     6481 cggatgcagg cattgactgc agcctttcaa aaaatagaat acattcctgt tcccacagag
     6541 tttttatatg cggatgacct ggttttcggt tatcgcctgg atattgcgta tgatgataag
     6601 cctgacacct ggtactccct gcacaaacgg aagatgagtt ttgcctttgc acctgtggat
     6661 cagccggtgc aaccgatcac gctggaaccg ggcgatgaac tggatgaagg atgtattcat
     6721 ttagcactaa ccgaagatga agaggatgga gatgagaaaa aagtaaatga agtgattgcc
     6781 cgctgggaag ggtggagttt atcggtacca cgtattggta aaggcatcaa caacgccgga
     6841 ccggaagtga gttcggatga agacgagaaa aagaaataca aactggataa ggaaaccccg
     6901 tttcgtttac aggtggaggt caaacccgcg ccgaagtcat taccgaaact acgttttggt
     6961 aaaaaatacc ggatcaaagt ccgcaccgtg gatattgccg gtaatggggt atcccatgat
     7021 gtacaaccgg agagtccgtc ccagactatc cgcagcggga ttgaataccg ccgttttgag
     7081 ccgctgagtt cgcctgtatt attgcaaggc gacgaaatca ccggcggcga taaaaaaatg
     7141 atccgcgacc gtgacgggga atccctgcag cacatggtga tccgcagcaa tgccggggtg
     7201 gatacaaaga cgtatgaaga taaaaacatc acgaccgtag tggtggatgg cagtgcaaaa
     7261 ggcacgttga agtatcacca cgaagcagtc cgttttatca cagcgccgcg tgggtcacag
     7321 cagctggccg aaatacacgg catgtttgac gaggccatga gtgatccgca gaaagcaatg
     7381 caggcctatc aatttatcac cagccgcgat aaagagtaca aggatgatgg taaaacgaaa
     7441 gcggtacaga ttcccgtgga aaccgggcag gtggatattg attatctcgc cgatccgctg
     7501 gctggcggag tagtctttac catgcggtcg aataccagct ttgaaacacc ctggaaaaaa
     7561 ggacagagcc gcaaattcag ttttcatttt gatgatgaag tgtctgaaaa tgatcccggg
     7621 aaaaaataca gtattgagca gtggaagaat ccccgttcgg tcaagatccg gctcgtggaa
     7681 ggcaatggtg agcccgaatg gaagaacagg gtgtttacca ttccacttcc caaagcttcg
     7741 attgtggaaa tcagttatgc cagtttctgg gcaccggaag cactggaacg tttttccgga
     7801 ttgatgccca ggttgcaggc gggtgccaat ggcgtgaaag taaaagagac ggcgaataaa
     7861 ggattacact ggatgtttag tccctggcgg aagatccggc tcgtgcatgc tgtgcaacag
     7921 ccactggaag caccggtgat ggcaaagatc tccaaagtgg aacgcgatta caatgatacg
     7981 gccgcccagt tgctcaccct cattaaagta catggagcaa gtaccgagaa aatggatctc
     8041 ggggccaaat ggacggagtg ggtggatgat ttatcaaaac ctgaaccgtt caaccaggcg
     8101 ttcagcaccc atgtggaaac tgtgccggtg gtatacaatg acaatatgct ggaactgatt
     8161 aaaacgccgg ccaccaaaaa cccgaagccg gattggttgc cgcctattac ccatctgttc
     8221 ggcgatacca aatatcgtca tgtggggtac atcccatccg cgactacccg tttccgggag
     8281 tattttaccg gaatcattga tactggtcgt attaaaggtg aacctttgcc actcagccgg
     8341 gaaggagaag tagtggaact cgatatactc agtaccgcaa gacctactgt gccggtgctg
     8401 gagtatgtgg tacccggctt tgtctggtcc aaatcacctg gccgtggccg taacaaagtg
     8461 aatatgcgca gtggaacgat aagggtctat ctgcgtcgtc cctggtacag cagcggggat
     8521 gatgagcgga ttgcggtgat ccttccgcca gaagggctgg agcccgaacg tcacccggag
     8581 tataaaaaat actgtacagt ttggggtatg gatccggtgt ttgagacaga gcggctgcca
     8641 aacagtttca gtaattaccc gcggaagaat cactttgtgg gtaccgatgt gatttatgat
     8701 acggtgaaac tggctgaaga aaatgtgccg gtgcaggtgg ccgcctataa agtatttttt
     8761 gataaagaga aacaactgca ttatgcggat atccgggtag atgcaggcaa tgcctatttt
     8821 ccgtttgtgc gactgatgct ggcccgttac cagcagcatt cattgcggga gaatggcacc
     8881 gatgtttgcc tgagtcagtc ggtacaggcc gattggatac aacaggttcc cctcaggcaa
     8941 gtatcctttg aagagttagc cggcagtttc cgtatacagc tgcaaggacc ttccgctttc
     9001 agatccggcg gaagggtgcg gataaatatt acagtggaga aagccctggt accaaaaacg
     9061 gataatgcat ttatcggcgt gaatgtaccc ggcgagcagt cagtcgtctt tacccgtgat
     9121 tataacctgg atgaaaagca gttaaatgct ggtaaaatca actttgagga aacagtacgc
     9181 gtgactggtg ccggcaatca atatcgggtg gtggtccgcg aatatgaact acatccgaat
     9241 gatccgttat tggccaaacc aaatatccag ggcaagaacg cagatacggt gggcgagcga
     9301 ctggtgttta tggaagtctt tgagtaaata cgccctttga tacgtcccgc atatagcagg
     9361 acaggcaggc tattggagag gggctaagca gatcccgtaa accccggtgc cgttattctt
     9421 ccttagtagc ggtatgctct tgtcatcctg cctgccgacc gtagcgcagc gtaggtggga
     9481 gtagtcccgc aaaaaaaata ttacctggct ggaataaata actccatgcg ggatgtatcg
     9541 aaggagaccg tctgtggacc gtcgaccatc gtctgtggac catccccaat acagacgagg
     9601 cccggaggcc tcgttgagcg acccatgagc ttcttcctgc gtcgccacag gcgacataat
     9661 acatccccgc agctgtaagc tgcggggccc gaaacaacaa ccgcagagga tgcagagggc
     9721 gcagaga
//
LOCUS       DAOPMW010000091         9572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7789_length_9572_cov_7.346328, whole genome shotgun sequence.
ACCESSION   DAOPMW010000091 DAOPMW010000000
VERSION     DAOPMW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7789_length_9572_cov_7.346328"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLU11_06280"
     CDS             <1..830
                     /locus_tag="PLU11_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPN58758.1"
                     /translation="KRTHMSAKKRILFIANEMSPYLELTEFSEIVNKLAIKANDNNFE
                     VRCIMPRFGIINERRHRLHEVVRLSGINVSVDNDDMPLQIKVASLPSARLQVYFLDNE
                     ELFKRKFVFHDEQEKWYEDNDLRTVFFCKGALETVKKFGWPPDIIHCSGWMTGLIPAY
                     LKTVYKKEPVFAHSKIVYTIGQNTFKEKLGSGLVKKALIHASLKEKDLELYKEGTNTA
                     MQRGGASFSDAITFGADKIDKKLTEEFTKVRGKKTLPYNPEGDLTDYLQLYSDLAGK"
     gene            862..2301
                     /locus_tag="PLU11_06285"
     CDS             862..2301
                     /locus_tag="PLU11_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58759.1"
                     /translation="MKYTKNAFPVIAIVLAVAILFPACRKINDATELGSGLIPPIDNI
                     NTFETFLDIESDNLLYNDTNKVYYADLHALGYISGDTEFGTTKGESYFNISYSNYLIN
                     PFYNKDSVIAIDSVVLSLGYDSYYGDTSSVQTVRVYELAQTSGFNDTTLYKYNQADFL
                     TTGSELGSKSFQVKNLNDTILHIRKRDTTKLVNVLRIPLVNTLGTRFMNYDTTFSANG
                     GFRSDSILKTLFRGLAIKADNSGNALTYISPSDNSNTRLTIYFQVKKNGIIDTTSVDF
                     QHSSGGQANIIRRTPGGGWNNYLTNTGTNDDLLYIPGTPGSYGLLRIPALDTFRNVVV
                     HRAEIIANPIRTAGDDMFPHQQALFLDRVSATGDSLLTFDADMLLSNNFTNYTYEINT
                     FGGLIKSDSTYRFNISRYVQSMVTNRTANYKMRLYTPVRTFVFSPGYNAINQIYVTDQ
                     PGYGRVVLAGGSYINPAKRLRMRLVYSKL"
     gene            2402..3658
                     /gene="metK"
                     /locus_tag="PLU11_06290"
     CDS             2402..3658
                     /gene="metK"
                     /locus_tag="PLU11_06290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPN58760.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAFDAQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIARGVIRKIGYTKSEYMFEASSCGVLSAIHEQSSDI
                     NQGVDRKKKEEQGAGDQGMMFGYATNETEHYMPLALDLAHNLLIELAAIRREKNSKMP
                     YLRPDAKSQVTLEYDDNNQPVRIDAIVISTQHDDFDSEAKMLARIKSDVINILIPRMK
                     TKYKKYAKLFNSDIKYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVASEVLVQVSYAIGVAKPTSINVNTYGTAKVDL
                     TDGEIGKIVEAVFDMRPYFIEQRLKLRNPIYSETAAYGHMGRLPQVVEKTFTSPDGKT
                     VTRKVELFTWEKLDYVSKVKKAFGLK"
     gene            3746..4513
                     /gene="rlmB"
                     /locus_tag="PLU11_06295"
     CDS             3746..4513
                     /gene="rlmB"
                     /locus_tag="PLU11_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPN58761.1"
                     /translation="MKQFRQPHRPKKNTLVIGRPAVMDALRNGQQLDRIYLDTMAQGT
                     DIRELKHLASQAGVPINYVPAAKLNSFNITGHEGCVAQIAKVQYQSLQDVISFVTESG
                     DAPLFLILDGITDIRNIGGIARTALCTGVQAIIIPDKGVGALNEDAILTSAGALETIP
                     VCRVNSLMKAVDELHLNGILVFASEMTAEKTISEVDFTIPCAIVLGGEEKGVYPALMK
                     ICDEKIRIPMKGDFESLNVSVATGMILYEAMTQRMKG"
     gene            complement(4514..5701)
                     /locus_tag="PLU11_06300"
     CDS             complement(4514..5701)
                     /locus_tag="PLU11_06300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN58762.1"
                     /translation="MKKIVLYLFVLPLFPCSCKKAQISITTQVGISSPWSDSSSRHPK
                     NTAFLNLLEKYKKKGFPGISLLVRDASGTWMGAAGKADLSNDIDFNPGTVSKAASITK
                     FFIGTLVFRLMEDSANTGMGYAALHRKVKDWLPARITDKLANGNQITLGHCLKHETGI
                     ADLINEDAFYLAVLNNPTKKWGPAELLEYIYDKPAEFSAGDTAVYSNTNTVLVTMILE
                     AQTGKKHSQLLHQYVLEPLGLKNTFYQPHDLLPRTTAQGYYDLYNNGSIVNVSNLVTG
                     SGNGYGGLYSNIYDLYTFIDAVLIRETFLKPASLTLMKSWGKRDDSNYYGYGIQQSYI
                     RGTDFGIGHKGRDLGYSANLFFFPARGVTHIFFVNYGTDADSRLKDIFFEFVDEMVAL
                     SLQ"
     gene            complement(5718..6335)
                     /locus_tag="PLU11_06305"
     CDS             complement(5718..6335)
                     /locus_tag="PLU11_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58763.1"
                     /translation="MNKYLMLLMLTGLSFKLDAQQQLKTKFISFSVMNTHTALPFGKF
                     TGIFYKAFHPGIEISYGKNFAVKNKHDWYYDFRLACFYHRFVQYGIPVYSNLGYRYKF
                     NQHWSAFTEAGAGYLHSIPATAKLRLNEAGDYENNKGPGRMQANACFALGAGYQLQGA
                     SANSLNLFISYQQRLQFPFVKSYVPLLPYNSFLLGLSRPLTRKNK"
     gene            complement(6492..7370)
                     /locus_tag="PLU11_06310"
     CDS             complement(6492..7370)
                     /locus_tag="PLU11_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58764.1"
                     /translation="MQTKTIICLILFLGFQFHKGICQQTEVNISSDVTEKKKINFFVV
                     SKRKKGKLDMATRYNVFRSKVRGFFCKRQFVSIVARDAKHASLKITKRLQANDAKLGT
                     IWFDSHGMYKKGYSLFYIGSDEISYKTLKDSTLIKYLSGLTSYADAESRLVIGSCYGG
                     ATYNRSSVDYKDTTRMNGDSLMIAIGNIVHCGKVYASESWVMTKPGLFRRRPSVGGFP
                     GRKLFRDLCYEPAWKNMGIWNKYDAEIGVLSRSNPVALDRYGNLIIRGVPFKHEKTFR
                     KSYHKKSSRLQTNLYK"
     gene            7572..8600
                     /locus_tag="PLU11_06315"
     CDS             7572..8600
                     /locus_tag="PLU11_06315"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN58765.1"
                     /translation="MNITDFSYISYAMETIELQQQLFQHLKENLPAHLSLVDELCSLL
                     DLSADSVYRRIRGEKPITLTELRVICEKYRLSLDKLIQLQTDSVLFDAPGLSGSTPEF
                     EDYLKGMLRQFHYFNSFEKREMFYLCKESTIWNFFLFPEMAAFKTFFWSKTINNQEKL
                     RNKTFSYEEFPYHECFQLGQKVLEEFNQIPCVELWNQESLQSTINQLVYYKESGNFSS
                     RKEYDRVLDSFQQLIDHLDLQTEKGVKFLPGANEVSYKAPIQFYVNEIILGSNNMVIN
                     LDGNSLTMVTYSVFHYLFTRDIRFRDKVMESFTTLLSRSTLISKTGEKDRNRFFNSLR
                     DKVHNLSK"
     gene            8630..9493
                     /locus_tag="PLU11_06320"
     CDS             8630..9493
                     /locus_tag="PLU11_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPN58766.1"
                     /translation="MISNQVRLIECPRDAMQGWKTPIPATQKAAYINLLLKAGFDTID
                     FGSFVSPKAIPQLADTREVIGQLDLSGTSTRLLAIVANLRGATDAVVFDEISYLGYPF
                     SVSETFQQRNTNSTIRESLTRVEEIQEICVKSGKQLVIYISMGFGNPYGDPYDEEQVF
                     EWVNRLVGMDIGIISLADTVGLASPEQVYSMTRYLVESLPGTEIGVHLHSTPDNWREK
                     LDAAVKAGCLRFDGALKGIGGCPMANDELVGNMDSERMIEYFRERNLLPDMDPVILNA
                     CTQMAAELFKH"
BASE COUNT         2684 a         2115 c         2134 g         2639 t
ORIGIN      
        1 aaaaaaggac gcacatgtca gcaaagaaaa gaattttatt tatcgcgaac gaaatgtcac
       61 cctacctgga gttgactgag ttttcagaaa tcgtgaataa gctcgcgatc aaagcaaatg
      121 acaacaactt tgaggtccgt tgtataatgc cccggtttgg catcatcaac gaacgccgtc
      181 accggctcca tgaggtagtc cgcttatcgg gtatcaacgt gtcggtagac aatgatgata
      241 tgccgctcca gatcaaagtg gcctccctgc ccagtgcccg gctacaggtc tatttcctgg
      301 ataatgaaga gcttttcaag cggaagtttg ttttccatga cgagcaggaa aaatggtatg
      361 aagacaatga cctgcgtacg gtgtttttct gtaagggagc cctcgaaaca gtaaaaaagt
      421 ttggctggcc cccggatatt attcattgca gtggctggat gaccggactg ataccggctt
      481 acctgaaaac ggtgtataag aaagagccgg tttttgcaca tagtaaaatt gtgtatacga
      541 tcggacaaaa cacgtttaag gaaaaactgg gaagcggact ggttaaaaaa gccctgatcc
      601 atgccagcct caaggaaaaa gacctggaat tgtataaaga aggtacaaat acggccatgc
      661 aacgtggagg tgccagtttt tcagacgcca tcacattcgg agccgacaag attgataaaa
      721 agctgactga agagtttacc aaagtgaggg gaaagaaaac ccttccatat aaccctgagg
      781 gagatttaac agactattta caactctata gcgaccttgc aggtaaataa aaatcactaa
      841 ccccgattat aaaatttctt tgtgaagtat acaaaaaacg ctttcccggt tatagcaatc
      901 gttctcgcgg ttgctattct gtttccggcc tgccgcaaaa tcaacgatgc tactgagctg
      961 ggcagcggtc ttataccacc aattgacaat attaatacct ttgaaacctt cctggatatc
     1021 gaatccgata acctgttgta taatgacact aataaagtgt attatgctga tcttcacgca
     1081 cttggttata tcagcgggga tacggaattt ggcaccacca agggggaatc ttattttaat
     1141 atcagttatt ccaattacct gatcaacccg ttttataata aagattcagt gatagcgatt
     1201 gattcggtgg tcctctcttt aggctacgac agctattatg gcgataccag cagtgtgcag
     1261 acggtccggg tttatgaact ggcgcagact tccggattca acgataccac gttatataag
     1321 tacaatcagg ctgattttct aaccaccggt tctgaactgg ggtctaaatc attccaggtt
     1381 aagaatctga atgataccat cctccatatc aggaagagag ataccaccaa actggtaaat
     1441 gtactccgga tcccattggt caatacactg gggacccggt ttatgaatta tgataccact
     1501 ttttccgcca atggcgggtt tcgctccgat tcaatcctga agaccctctt tcgcggcctg
     1561 gccattaaag cagataatag tgggaatgcc ctgacgtata tcagtccttc tgataatagt
     1621 aacacccgtc tcacgatcta ttttcaggtc aagaaaaacg ggattattga taccacgtcg
     1681 gtcgacttcc agcactcctc aggcggacag gcaaatatca tcaggcgtac accggggggt
     1741 ggatggaata attacctgac caataccggt accaatgacg acctgcttta tattccgggt
     1801 accccgggat cctacggttt gctccgtatt ccggccctgg atactttccg gaatgtggta
     1861 gtccaccggg cagaaattat agccaatcct atccgtactg caggggatga tatgttcccg
     1921 caccagcagg ccctgttcct ggaccgggtc agtgccacag gggactcctt actgaccttt
     1981 gatgccgata tgttgttgtc caataacttc acgaactata cttacgaaat caacacgttt
     2041 ggcgggttga tcaagtcgga cagtacctac cggtttaata tatcgaggta tgtacagagt
     2101 atggtaacca accggacggc taattataaa atgcggttat atacccctgt cagaaccttc
     2161 gtcttttcac caggctataa tgccatcaat cagatctatg tgacagatca gcctggatat
     2221 ggaagggtgg tactggccgg cggcagctat attaacccgg caaaacggct gcggatgcgc
     2281 ctggtctatt ctaaattgta acaggtgatc aaaatatgtt caaaagtcct tcctttcacg
     2341 ggaagggctt tgttctttat aaatgccccc ttatctttgc gtccttaaaa actattaact
     2401 catgccttat ttatttacct ctgaatccgt aagtgaaggt catcctgata aagtggccga
     2461 ccagatttcc gatgcactca ttgataactt cctggctttt gatgcacagt caaaagtggc
     2521 ttgcgaaacc ctggttacta ccggacaggt ggtgcttgcc ggtgaagtaa agtcaaaagc
     2581 ttacctggac gttcaggaaa tcgcccgcgg agtcatccgt aaaatagggt ataccaagag
     2641 tgaatacatg tttgaagcca gcagttgcgg ggtattgtct gctattcacg aacaatcttc
     2701 cgatatcaac cagggtgtag accgcaaaaa gaaagaagag cagggtgccg gtgaccaggg
     2761 aatgatgttt ggctatgcca ccaacgagac agagcactat atgcccctgg cacttgacct
     2821 ggcacataat ctcctgatcg aactggctgc tatccgtcgc gaaaagaact ctaaaatgcc
     2881 ttacctgcgt ccggatgcca aaagccaggt aaccctggaa tatgatgaca ataatcagcc
     2941 cgtacgtatt gatgcgatcg ttatttcaac ccagcacgat gattttgaca gcgaagctaa
     3001 aatgctggcc cgtattaaaa gcgatgtgat caacatcctg attccccgga tgaagaccaa
     3061 atacaagaag tacgcaaagc tgttcaacag cgatatcaaa taccacgtta acccaactgg
     3121 taagtttgta atcggtggtc cgcatggtga taccggtctt accggccgta aaatcattgt
     3181 ggatacctac ggtggaaaag gtgcacacgg aggtggagcc ttcagtggta aagatccttc
     3241 taaagtagat cgttctgccg cttatgccac ccgtcatatt gccaagaacc tggtagcagc
     3301 cggcgtagcc agtgaagtgc ttgtacaggt ttcatacgcg atcggagtgg ccaagcctac
     3361 cagtattaac gtgaatactt atggtaccgc aaaagtggac ctgaccgacg gagaaatcgg
     3421 gaagatcgtg gaagccgtat ttgacatgcg tccttacttt attgaacaac gcctgaaact
     3481 gcgtaatccg atatacagcg aaactgctgc ttacggacat atgggtcgtt tgccccaggt
     3541 ggttgagaaa accttcacct ctcctgatgg caaaacggta acccgtaaag tagaactctt
     3601 tacctgggag aaactggatt atgtcagcaa agtgaaaaaa gctttcggtc ttaaataaac
     3661 tgaacagtct catttaaaaa ccctggaaga tcctgtcttt tcagggtttt ttatttccac
     3721 atcacgaatc gcttaatttt acacggtgaa acaattcaga caaccccatc gtcccaaaaa
     3781 aaatacactc gtcattggcc gcccggctgt aatggatgca ttacgaaacg gacagcaact
     3841 cgaccggatt tacctggata caatggccca gggtaccgat atacgggaac taaagcacct
     3901 ggcttcccag gctggtgtac ccatcaatta tgtacccgca gctaaactca acagttttaa
     3961 tattaccggt catgaaggct gtgtggcaca aatagccaaa gtacaatacc aatccctgca
     4021 ggatgtaatt tcctttgtca cagaaagcgg ggatgcacct ttattcctga tccttgatgg
     4081 catcacggat atccggaata tcgggggaat tgcccgtaca gctctgtgta ccggggtgca
     4141 ggccattatc atacccgata aaggagtagg ggccctgaat gaagatgcca tcctgacttc
     4201 cgccggcgcg ctggaaacga ttccggtttg ccgtgtaaac agtctgatga aagcggtcga
     4261 tgaacttcac ctgaacggta tcctggtgtt tgccagtgaa atgacggctg aaaagacaat
     4321 cagtgaggtc gattttacca tcccctgtgc gattgtattg ggtggagaag aaaaaggcgt
     4381 ttaccctgca ctgatgaaaa tatgtgatga gaaaatcagg attcccatga aaggtgattt
     4441 cgaaagcctg aatgtatctg tggccaccgg aatgatcctg tatgaagcga tgacccagcg
     4501 gatgaaaggc tgatcattgc aggctcagtg ctaccatttc gtctacaaat tcaaagaaaa
     4561 tatctttaag cctgctgtca gcatcggtac cgtaattgac aaagaagata tgcgtaacac
     4621 cccgtgcggg aaagaaaaac aggttggctg aataaccgag atcccttcct ttatgtccga
     4681 ttccaaagtc ggtaccccga atatatgatt gctgaattcc atacccgtag taattactgt
     4741 cgtccctttt tccccagctt ttcataaggg tcagtgatgc cggcttcagg aaagtttcac
     4801 ggatcagcac cgcgtcaata aaagtgtaca gatcataaat attgctgtat aacccgccat
     4861 atccattccc gctgccggtt acaagattag atacattcac aatactgcca ttattataga
     4921 gatcataata cccctgtgca gtggttcgtg gcaacagatc atgtggctga taaaatgtat
     4981 ttttcaaacc caatggctcc agtacatact ggtggagcaa ctgactatgc ttttttcctg
     5041 tctgtgcctc caggatcatg gtgacaagta ctgtattggt attggaataa actgcggtat
     5101 caccggctga aaattcggcc ggtttatcat agatatactc cagtaattcc gctggtcccc
     5161 attttttagt tggattattg agaacagcca ggtaaaaagc gtcttcattg atcaggtccg
     5221 caatgcccgt ttcatgtttc aaacaatgtc cgagcgtgat ctgattcccg ttggccagtt
     5281 tgtctgtaat tctggccggc aaccagtctt ttaccttgcg gtgcagtgcg gcatatccca
     5341 ttccggtatt agcagaatct tccatcaggc gaaaaaccag ggtgcctatg aaaaatttgg
     5401 tgatactcgc tgctttggat accgtacccg gattaaaatc gatatcattt gaaagatccg
     5461 ctttaccggc ggcacccatc caggtaccgg atgcatccct gaccaggagg gagatacccg
     5521 gaaacccctt ctttttatat ttttccagca ggttcaggaa agcggtattt ttcggatgac
     5581 ggctgctgct gtcagaccat gggctgctga tacccacctg ggtggtaatg ctgatctgtg
     5641 cctttttaca agagcaaggg aagagcggca gtacaaaaag gtaaagcacg atttttttca
     5701 tacgaatagt ttaaaggcta tttgtttttt ctggtcagtg gtctggataa tcccaggaga
     5761 aaactgttat agggaagaag gggtacatag gacttcacaa aaggaaattg cagccgttgc
     5821 tgataactga taaacagatt gagcgaattg gcggaagctc cttgcaactg ataacctgct
     5881 cccaatgcga agcaggcatt ggcctgcata cgtccggggc ctttattatt ttcatagtca
     5941 cctgcctcat tcaaccgtag tttggccgta gcaggaatgg aatgcaggta tccggcaccg
     6001 gcttcagtga aagcagacca gtgctgatta aatttatacc gataacccag gttgctgtaa
     6061 accggtatac catactgtac aaaccggtga tagaaacagg caagccggaa atcgtaatac
     6121 cagtcatgtt tgttttttac agcgaagttt ttcccatacg aaatttcaat acccggatga
     6181 aacgctttgt aaaatatacc ggtaaacttc ccgaagggaa gagctgtgtg ggtattcatt
     6241 acggagaaac ttataaactt tgtttttaac tgctgctgcg catccagttt aaaactaaga
     6301 ccggttaaca tcagaagcat gagatattta ttcataacca gcgaatttat ctgtttaacg
     6361 ttcagtgtta agaaccagga tttcaagtta attttcccgg attctattca tttaatttca
     6421 tccgtcgtgc ttttaaactt gcagaccttc ccttataatc actttcaaat acttatatac
     6481 ttgtacctgt tttacttata cagatttgtc tgaagccggg atgatttttt gtggtagctt
     6541 ttcctgaacg ttttttcgtg tttgaatgga actccacgga tgatcagatt cccatacctg
     6601 tcgagtgcta ccggattact tcggcttaat actccgattt ctgcatcata cttattccag
     6661 attcccatat ttttccaggc cggttcgtaa cataaatccc tgaacaattt gcgtccggga
     6721 aatcctccaa cggatggtct gcgacggaat agaccaggtt tggtcattac ccagctttca
     6781 gatgcatata ctttgccgca gtgtactata ttgccaatgg caatcatcag cgaatctcca
     6841 ttcattcttg tcgtgtcctt atagtcaacc gaagaccgat tataagtggc acccccataa
     6901 caggatccga taaccaatct tgattctgca tccgcatagg aggttaagcc cgataagtat
     6961 ttaataagag tactgtcttt tagagttttg taacttattt catcacttcc gatataaaat
     7021 aatgaatatc cctttttata cataccatga gaatcaaacc agatggtacc taacttggca
     7081 tcattagctt gcaggcgctt agttattttt aacgatgcgt gttttgcatc acgtgcaacg
     7141 atggaaacaa attgtctttt acagaagaag cctcttactt ttgatcgaaa cacattataa
     7201 cgggtcgcca tatccagttt cccttttttt cttttactca ctacaaagaa gttgattttc
     7261 tttttttctg ttacgtctga tgaaatattt acttcagttt gctggcaaat acctttatga
     7321 aactggaatc ccagaaacag tatcaggcaa atgatcgttt tagtttgcat ggtgttgctt
     7381 taagatgatc aggtttgttg acctatcaaa aatcagcagg tacatccgtt tgaaataatt
     7441 ggttttagca ttactataat ctaaaatggt agttatcagt gatttttctg ccaggtgatc
     7501 cagatttgga tttttcccgt attataaaat gtgtttcggg cagccttttg cagttttccg
     7561 taaacatttt tttgaacatt acggattttt cgtatatttc ttatgcaatg gaaactattg
     7621 agcttcaaca acaattgttt cagcacctga aagagaacct gccggcacac ctttcactgg
     7681 tagatgaact atgttcatta ctggatctca gtgcagatag tgtttaccgc cggataaggg
     7741 gtgaaaagcc gatcacactt actgagttac gtgttatttg cgaaaaatac cgtttgtcac
     7801 tcgacaaact cattcagtta cagactgatt ctgttttgtt tgatgccccc ggattaagcg
     7861 gaagtacgcc ggagtttgag gattacctga agggaatgct ccggcaattt cactatttca
     7921 attcgtttga aaagcgggag atgttttatc tctgtaaaga atccaccatc tggaatttct
     7981 ttctgtttcc ggaaatggcc gcttttaaaa cttttttctg gtccaaaaca attaataatc
     8041 aggaaaaact ccggaataag acattctctt atgaagagtt tccgtaccat gagtgtttcc
     8101 agttaggcca gaaagtcctg gaagaattta accagatccc ctgtgtggag ttatggaacc
     8161 aggaaagtct gcagagtaca atcaatcagc tggtctatta taaggaatcc ggcaacttca
     8221 gttcacgtaa ggaatatgac cgtgtacttg attcattcca gcaactgatt gatcatctgg
     8281 atctgcagac tgaaaaaggg gtgaaatttc tgccaggggc caatgaagta tcttataaag
     8341 cacccattca gttttatgta aatgagatta tcctgggcag taataatatg gtcattaatc
     8401 tggatggcaa tagcttaacg atggttactt acagtgtatt tcattattta tttacaaggg
     8461 atatccggtt ccgggataaa gtcatggaat catttactac actgcttagc cgttcaaccc
     8521 tgatcagtaa aaccggcgag aaagacagga accgcttttt taactccctt cgggataagg
     8581 ttcataacct ttcaaagtaa ttagccagac ggtcttaact tcgcatgaaa tgatttcaaa
     8641 tcaggtcaga ttgatcgaat gtccgcggga tgccatgcag ggctggaaga cacccattcc
     8701 tgccacgcag aaagccgctt atatcaatct gttgctgaaa gccggttttg ataccatcga
     8761 ttttggcagt tttgtttcac ccaaagctat tccccaactg gcggataccc gtgaagtgat
     8821 cgggcaactg gatctttcag gtacctccac caggttactt gcaattgttg caaatctccg
     8881 gggtgcaacg gatgccgttg tatttgatga aatcagttac ctgggctacc ccttctccgt
     8941 ttccgaaact tttcagcagc gaaacaccaa cagtaccatc cgtgaatccc tgacaagggt
     9001 ggaagagatc caggaaatct gtgtaaaatc aggtaaacaa ctcgtgattt atatttccat
     9061 gggctttgga aatccgtatg gcgatcccta tgacgaagaa caggtatttg aatgggtcaa
     9121 ccggctcgtg ggaatggata tcggcattat ttcactggca gatacggtag gactggccag
     9181 tccggaacag gtttattcga tgacccggta tttggtggaa tcacttccgg gaactgagat
     9241 cggcgtgcac ctgcactcga cacccgacaa ctggcgggaa aaactggatg ccgcagtaaa
     9301 agcaggctgt cttcgttttg acggggcact gaaaggaatc ggcggctgtc cgatggccaa
     9361 tgatgaactg gtaggtaata tggatagcga acggatgatt gaatatttca gggaaagaaa
     9421 cctgttaccg gatatggatc cggttatcct gaatgcctgt actcaaatgg ccgctgaact
     9481 ttttaagcac tgatcatgga ctttattctg gatatagcgc tggaaaaact gcctgaaccg
     9541 atccgttacc aggatcaaat aatgctgatc gg
//
LOCUS       DAOPMW010000092         9418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_7964_length_9418_cov_9.782442, whole genome shotgun sequence.
ACCESSION   DAOPMW010000092 DAOPMW010000000
VERSION     DAOPMW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_9418_cov_9.782442"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1137
                     /locus_tag="PLU11_06325"
     CDS             109..1137
                     /locus_tag="PLU11_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPN58767.1"
                     /translation="MKLNRNNKPDFIRTFINYFLGPLLFIWLSWSIYTEIKNQPDIEG
                     AWLQVRQSFSSPLLLIIPGVIFLMIINWAIEAIKWQLAVKPVQPVSFLKAFKAVLSGV
                     SFSVSTPNRVGEYLGRVLYMEEGNRLRTIAVTMVSSMSQLIITLCMGLLGLILLKKTI
                     EQQQLVSGLWLLVLIYGVLIVLVLLMLFYFRLHWLARWISKISFLKKYSYLVDAVGQF
                     DTSILLRMLALSFARFAVFILQYYLLYIFFDVQIGWWAACWTVSVSFLIMAIIPTFAI
                     AELGLKGKVALLVTGLFSANKAGIFFATAGIWFINLVIPAILGSLLILGLRKFYRMPD
                     QSSDNLKN"
     gene            complement(1110..1970)
                     /locus_tag="PLU11_06330"
     CDS             complement(1110..1970)
                     /locus_tag="PLU11_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN58768.1"
                     /translation="MKLYTINTGYFKLDGGAMFGVVPKSIWNKINPADENNLCDWALR
                     CMLIEDEGRLILVDTGNGDKQDAKFFGHYHLHGDDTLEKSLNSHGFHPDDITDVFLTH
                     LHFDHCGGAIKRAGDQLVPAFKNAKYWSNAAHWDWAVYPNAREKASFLKENILPIKES
                     GQLEMVEVNGGINGQLGKTRFTDNIAVRFVSGHTESMMLPQIQYKGRTVVYMADLLPS
                     QGHIPLPYVMGYDMFPLTTLNEKTSFLKEALEGDYVLFFEHDPIYECCTLKQTERGIR
                     PDQFFKLSEL"
     gene            complement(2081..3379)
                     /locus_tag="PLU11_06335"
     CDS             complement(2081..3379)
                     /locus_tag="PLU11_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN58769.1"
                     /translation="MQTAPKKVVNAWAMYDWANSVYNLVITTTFFPAYYSAVTKPENE
                     GFENGVHFLGRNFVNTELKDYVLAFGFLVIAAASPILSSIADYKGNKKIFMRWFCYLG
                     ALSCSMMIFFDETNVGFGLLCFMFAGIGFYGSQVFYNSYLPDIAAEADRDRISARGYS
                     FGYIGSVLMQMIGFALVMLMEDKGLALQLTFLLVGIWWIAFAQIPLKWLPISSRSERK
                     SKKHVLVNGFHELKLVWNQLVHMPVLKRFLSAFFFYSMGVQTVMLVAIDFGLKELKLA
                     DDKLIITAVIIQLVAIAGAIGMSRLSEKFGNIRILLLTVILWIGVCITGYFITTEFHF
                     YILAALVGLVMGGIQSLSRSTYSKLMPETKDTTSFFSFYDVTEKLAIVIGLFSFGLIE
                     GLTGSMRNSIIALIVFFVLGLVFLLLTSKVSTASNRPAAV"
     gene            complement(3480..4220)
                     /locus_tag="PLU11_06340"
     CDS             complement(3480..4220)
                     /locus_tag="PLU11_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58770.1"
                     /translation="MKKYILLLLVPFLAACSNYGSKATSDKIEVYYKDGISKEQAENT
                     ARLFDQLLNETSPDDHSKKSFQLLKNGDTILLKMVADKSKLSAVGDDAFYAIATMVSD
                     SVFAGAVVNLTLTDNTFKGFKNYAYRKNAVSSWGEKYTDGLIEVYDDGVGTPAVKELA
                     SFMNSYFSPQTTFSFQLTTNEAKEVIARMVANPDRMSFFTEDVLQEISKGISDKVFNG
                     SPVHFELTDTKFKPLRRYSYPADAAGMQ"
     gene            complement(4287..5087)
                     /locus_tag="PLU11_06345"
     CDS             complement(4287..5087)
                     /locus_tag="PLU11_06345"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPN58771.1"
                     /translation="MTTIEAIIIAIVEGLTEFLPISSTGHMIIAGKLLGVEDNDFTKL
                     FTVGIQLGAILAVVVLYWKKFMTPLTQGKKGWNFYLKLLVAVLPALGLGFLLSDYIDT
                     LLESSLTVAITMILGGIVLLFIDNVFKDHPITTEEQISFPTAFKIGLWQCVAMIPGVS
                     RSAASIIGGMQQKLTRKLAAEFSFFLAVPTMAAATGYKMLKGYKSLGAEDIKLFAIGN
                     VVAFIVAMLAIKFFIGFLQKHGFRLFGYYRIIGGIVLLVLLATGYLKS"
     gene            complement(5192..5434)
                     /locus_tag="PLU11_06350"
     CDS             complement(5192..5434)
                     /locus_tag="PLU11_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPN58772.1"
                     /translation="MSESKNTSMFSKENYIWMLAGLILIALGMFLMSGGKSNTDPAVF
                     NKEEVYSPIRITVAPILILAGLIVEVFAIFKKPKAA"
     gene            complement(5582..6454)
                     /locus_tag="PLU11_06355"
     CDS             complement(5582..6454)
                     /locus_tag="PLU11_06355"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPN58773.1"
                     /translation="MSQAGKASIKRGKPSYFMSILGVTLVLFFLGIIGLIVINTNKLS
                     DHFKENVTVSAYLRGDLNPKDSTELVNYVSAKPYIREFTYVTKEIAKKKYLADGNKDW
                     SEVLNENPLPNSIDFKVKNEYLNTDSLTAIKADLKAQTYVSDVEYPEALISQMSTNLR
                     NISLGILIVAIVLAIVVIVLIDNTIKLAMFSNRFLIKTMQMVGATRWFIAKPMNVRAV
                     INGALSGVIAAVAVILFLMVIEKFIPEMKAIHDNTSLVLLFVGLIFLGIAITVFSTHR
                     SVIKYLKMKLDDLY"
     gene            6711..>9418
                     /locus_tag="PLU11_06360"
     CDS             6711..>9418
                     /locus_tag="PLU11_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPN58774.1"
                     /translation="MEYQFRDIEKKWQAKWKADNAYQVNNDFSKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGFDSFGLPAEQYALDTGQHPAVTTKK
                     NIETFKKQLDNIGFCFDWEREVQTSDPSYYKWTQWIFLQLFGSFFNRQTAKAESIDSL
                     VAVFEKEGNSAHACPGDEKIKFTAAEWKSFDEKQQQDILMQYRLAFCGYGEVNWCEAL
                     GTVLANDEVVNGVSERGGHPVVKKKLRQWYLRITEYADRLLEGLERIEFSDAMKEMQT
                     NWIGRSSGAEIDFEVKKPPLSPPKGEETAPRYQTATSSLYSITKSLAEEKRKNPTSEE
                     QILWQLLRANKTGFHIRRQHIIGSYIVDFACLDKMVTIEIDGGYHNNPEIQFYDKERT
                     RFLTGQGFTEVRFTNEDVNRDPETVVKKITDLLSNTVSVLPKTGSSEVSPLGGDLEGA
                     LKLRVYTTRPDTIFGVDFMVVAPELDIVEELKSTEQAAAVDEYLAYVKSRSERERMAE
                     KKISGVFTGAYAINPFDGREIPIWISEYVLAGYGTGAIMAVPCGDERDHKFARHFNIP
                     ITNIIGAYYNGEEANPTKEATLENSGFLNGLLMKDAIEVAVTAIEEKGIGTRKKNYKM
                     RDAAFSRQRYWGEPFPIVWKNGIAYPLSVDQLPLELPVVDKYGPGPDGEGPLANIKEW
                     FGQFESEIPPLGGGGALETNTMPGYAGSSWYFLRYMDPKNDTNFCDRKVSDYWNQVDL
                     YIGGTEHAVGHLLYSRMWTKALFDLGHIGYDEPYKKLVNQGMIQGSSRFVYRIAGTNQ
                     FVSAGLKDQYETNAIHTDVNFVDGFELDIEAFKKWREEYTDAEFILEDGKYICGSEVE
                     KMSKSKYNTVNPDDLVYKYGADTFRMYEMFLGPVELSKPWDTKGIEG"
BASE COUNT         2598 a         2180 c         2145 g         2495 t
ORIGIN      
        1 cggccggtta cttttaccag cccgtaaccc attacaatgg ttccggggtc aatacccaat
       61 attattttaa caggcttttc cagcagggag tgtattttta caagattaat gaagctgaac
      121 cgaaataaca aacccgattt catccggacc ttcatcaact acttcctcgg tccgctcttg
      181 tttatctggc tttcatggtc aatttataca gaaataaaaa accagccgga tattgaaggg
      241 gcctggctgc aggttcgtca atcattcagc agcccattat tattaattat acctggtgta
      301 atcttcctca tgatcatcaa ttgggccatt gaagccatca aatggcaatt ggctgttaaa
      361 ccggtacagc cggtatcctt tctcaaagca ttcaaggccg tgctctccgg cgtatctttt
      421 tcggttagta cacccaaccg ggtgggcgaa tacctgggcc gggtcttgta tatggaagaa
      481 ggtaaccggc tgcgtaccat cgccgtgacc atggtaagca gtatgagtca gctcatcatt
      541 accctgtgta tgggtttgct gggacttatc ctgttgaaaa aaacgatcga acaacagcag
      601 ctggtatccg gcctgtggtt gctggtgctg atttatggcg tgctgattgt tttggtactg
      661 ttgatgttgt tttacttccg cctgcattgg ctggccagat ggatatcaaa aatttctttt
      721 ctgaaaaagt atagttatct cgtggatgcc gtgggtcagt tcgatacctc catcctgctc
      781 cggatgctgg cgctttcttt tgcccggttt gctgttttta tcctgcaata ctacctgctc
      841 tatattttct ttgatgtaca gatcggttgg tgggctgcct gctggacggt cagtgtctcc
      901 tttctgatta tggcgatcat cccaactttt gccattgcag aacttggtct taaaggcaaa
      961 gtggccttgc tggttaccgg actcttcagc gccaataaag cgggcatctt ttttgccacc
     1021 gccggtatct ggttcatcaa tctcgtgatc ccggctattc tcggcagtct gctgattctc
     1081 ggtctgcgta aattttaccg gatgccggat cagagttccg acaacttgaa gaactgatca
     1141 ggccggatac cccgctctgt ttgttttaac gtacaacatt catatatggg gtcatgctca
     1201 aaaaacaata cataatcccc ttcaagggct tcttttaaaa aacttgtttt ttcatttaat
     1261 gtggtcaggg gaaacatatc ataacccatc acatagggca ggggtatatg tccctgcgaa
     1321 ggcagcagat ccgccatata tacgaccgta cgtcctttgt attgtatttg tggcagcatc
     1381 atgctttctg tatgaccact cacaaaccgg acagctatat tatcggtaaa acgggtctta
     1441 cccagctggc cattgatgcc cccgtttact tccaccattt ccagctgccc gctttctttt
     1501 atgggcaaaa tattttcttt caggaaagag gctttttccc gcgcattcgg gtataccgcc
     1561 cagtcccaat gggcggcatt gctccagtat ttcgcatttt taaatgcagg taccagttga
     1621 tcacccgctc ttttaatagc accaccgcaa tggtcaaaat gcaggtgcgt caggaagaca
     1681 tccgtaatat catccggatg aaagccatgg ctattcaggg atttctccag ggtatcatca
     1741 ccatgtaagt gataatggcc gaaaaatttt gcgtcctgtt tatcgccgtt tcccgtatct
     1801 accaggatca gccggccttc atcttcaatc agcatacagc gcagggccca gtcgcaaaga
     1861 ttattttcat cggctggatt gatcttattc cagatggact tcggcaccac accaaacata
     1921 gcaccaccat ctaatttaaa gtatccggta ttgattgtat agagtttcat agttataata
     1981 ttttttcccg caaaggacgc aaagattttt gtttatagag agcatcttac atcttccgat
     2041 actcttttat gctttgcggg caaactttaa aagcgtggcc tcatacagca gcgggccggt
     2101 tggaagccgt agacacctta ctggtcagca gcaaaaatac cagtcctaac acaaaaaaga
     2161 caatcagtgc gataatcgaa ttacgcatgc tgcccgtaag tccttcgatt agtccaaaac
     2221 taaatagtcc gatcacgatg gccagttttt cagtcacatc ataaaaacta aagaaggagg
     2281 tggtgtcttt agtttcaggc ataagtttgg aataggtact acggctgagt gattgtatgc
     2341 cgcccataac gagtccgact aatgcggcca gtatataaaa atgaaattca gtggtaataa
     2401 aatacccggt aatacaaacg cctatccaga gtataacggt cagcaggagg atgcggatat
     2461 ttccgaattt ttccgataac ctcgacatcc cgatggctcc ggcaatggcc accaattgaa
     2521 ttatgactgc ggttatgatc agtttatcat ctgccagttt taattccttt aaaccaaaat
     2581 caatagccac cagcatgacc gtctgcaccc ccatactgta aaaaaagaat gcagatagaa
     2641 aacgtttaag tactggcata tgaactaatt ggttccagac cagttttaat tcatgaaaac
     2701 cgttgaccag tacatgtttt ttggatttac gctctgagcg gctgctgatg ggtaaccatt
     2761 tcagcggaat ctgggcaaat gcgatccacc agatgcccac caggagaaaa gtgagctgaa
     2821 gagccaggcc tttgtcttcc atcagcatca ccagcgcaaa accgatcatt tgcatcagta
     2881 cactcccgat atagccaaat gaataacctc gggcgctgat ccggtcccgg tcggcttcag
     2941 ccgcaatgtc cggcaggtat gagttataaa atacctggct gccgtagaaa ccaataccgg
     3001 caaacataaa acagagcaaa ccgaatccta cattggtttc atcaaagaaa atcatcatcg
     3061 aacagctcag ggcgcccagg tagcaaaacc agcgcatgaa aattttctta ttccccttgt
     3121 agtcggcaat ggatgaaaga atcggtgaag cggcggcaat taccaaaaag ccaaaagcca
     3181 gtacataatc tttcagttcg gtgttgacga aattccgccc gagaaaatgc accccgtttt
     3241 caaaaccttc attttcaggt ttggtaacag cggaataata agcgggaaag aaagtggtcg
     3301 tgattaccag gttgtacact gaattcgccc agtcgtacat cgcccaggcg tttactactt
     3361 ttttcggagc cgtctgcatg aatgcttgct ttaggtaaat ataggaaatt ggcacacaat
     3421 ccaaaaaaag gggccatacc attaccggta cagcccatag gtttaaaagg gtatatcttt
     3481 tactgcatgc cggctgcatc tgccggatag gaataacgcc gcaggggttt aaactttgta
     3541 tcggtcagtt caaaatgaac cggtgacccg ttaaatacct tgtccgaaat gcccttgctg
     3601 atttcctgca acacatcttc agtgaagaag ctcatcctgt caggattcgc caccatccgg
     3661 gcaatcactt cctttgcctc gtttgttgtc aattggaaac tgaacgtggt ctgcggactg
     3721 aaataactgt tcataaaaga agccagctct tttactgccg gggttccaac accatcatca
     3781 taaacttcta ttaatccatc ggtatacttt tcgccccagg aggacactgc attcttccgg
     3841 taggcataat tcttaaatcc cttaaatgta ttatccgtga gcgtaaggtt gaccactgcc
     3901 ccggcaaaca cactgtcgga taccatggtg gcaatggcgt aaaacgcatc atcccccaca
     3961 gcactgagtt tggatttatc cgctaccatt ttaagtaaaa tggtatcccc gttttttaat
     4021 aactgaaaac tttttttgga atggtcatcc ggactggttt cattcagcaa ctgatcaaac
     4081 aaacgggcag tattttccgc ctgttctttg ctgattccat ctttgtaata cacttcaatt
     4141 ttgtcagaag tggcttttga accataattg ctgcaggcgg ccaggaaagg aacaagcagc
     4201 aggaggatat actttttcat actgttattt acacgcatag ataggaaatg aatgcggaac
     4261 gctgactacc ctgttcaggg gatatttcag cttttcaggt acccggtggc caggagcacc
     4321 agtaacacaa taccacctat gatccggtaa tagccaaaca ggcggaaacc atgcttctgc
     4381 agaaacccga taaagaattt aatcgccagc atggctacga taaaggctac cacattcccg
     4441 atggcaaaga gttttatatc ctcggccccc agtgatttat atcctttcag catcttataa
     4501 cccgttgcgg cagccatcgt gggaaccgcc aggaagaagg aaaattcagc cgccagcttg
     4561 cgggtcagtt tctgttgcat accccctatg atactggccg cactccggct tacccctgga
     4621 atcatggcca cacattgcca gagtccgatt ttaaatgcgg tggggaaact gatctgttct
     4681 tcggtagtga taggatgatc tttaaaaaca ttatcaataa ataaaagcac aattccgccg
     4741 agtatcatcg taatcgccac ggtgagcgaa ctttccagca gcgtatcaat ataatcggat
     4801 aaaagaaaac ccagtcccaa tgccggtaaa acagccacca gcaatttcag gtaaaagttc
     4861 cagccttttt tcccctgggt cagcggggtc atgaattttt tccaatataa taccaccacc
     4921 gccaggatag ctcccagttg aatacctacc gtaaagagct tggtaaaatc attgtcttcc
     4981 acacccagca gtttcccggc aatgatcata tgaccggtcg atgaaatagg caggaactcg
     5041 gtcagtcctt ccacgatggc aatgatgatg gcttctattg ttgtcatgag gttgtttttg
     5101 gcttggaagc gtaaaggtat gtctttaaaa aaaggctaaa aaaaagaggc tgatcggatg
     5161 gatacagcct cttacttatt taatccgcca ctcaggcagc tttgggtttc ttaaaaatcg
     5221 caaaaacttc cacgatcagc ccggccagga tcagtatcgg ggctacggta atccggatag
     5281 gactataaac ttcttcttta ttaaaaacgg ccggatcggt attacttttc ccacccgaca
     5341 ttaaaaacat tcccagggca atcaggatca gcccggccag catccagata tagttttctt
     5401 tgctgaacat cgatgtattt tttgattcac tcatttgggg tcattttagg aggtgcaata
     5461 tagggaaaag ctagaagcca gaagcaagaa gccagaaggg tcttttttta ggactatatc
     5521 tttaaatatc aggttcatac tgctttagga taccttctcg cttctcgttt ctcgcttctc
     5581 gctaatatag atcgtccagc ttcattttca agtacttaat cacactccgg tgggtgctga
     5641 atacagtaat ggcaataccg agaaagatca gaccaacaaa aagcagcacg aggctggtat
     5701 tatcgtggat ggctttcatt tcaggaataa atttctcgat gaccatcagg aacaggatca
     5761 ccgctactgc cgcaatcacc ccactcaggg caccattgat caccgccctt acattcatcg
     5821 gtttggcaat aaaccagcgg gtggccccaa ccatctgcat ggtcttgatc aggaaacggt
     5881 tgctgaacat ggccagtttg atggtattat cgatcagtac aatcaccaca attgccagca
     5941 cgatggccac gatgaggatg cccaggctga tattacggag attagtactc atctgactga
     6001 tcagtgcctc gggatattcc acatcactca cataagtctg ggctttcaga tcagctttga
     6061 tagcggtcag cgaatctgtg ttgaggtatt cgttttttac tttgaagtcg atactgttgg
     6121 ggagaggatt ctcgttcagc acttctgacc aatccttatt accatccgcc aggtatttct
     6181 ttttcgcaat ctctttagtt acatacgtga actcccggat atagggtttg gcacttacat
     6241 aattgaccag ttccgtactg tccttgggat tcaggtcacc ccgcagatag gcactcacgg
     6301 ttacattttc cttgaaatga tccgacagtt tattggtgtt gataacgatc agcccgataa
     6361 tgcccaggaa gaacaaaacc agggttacac ccaggataga cataaaatag gacggtttcc
     6421 ctcttttgat cgaggcttta cctgcttggg acatgcgatt tcttttagcg atgaatggat
     6481 tacggcaaaa atagcctttt acacccaatc ctgttgcctt ttcaggggct ggatttgccg
     6541 ggttcaggct gttacagata cagccgatct ttcgggctgt attcagtatt tttgcacccc
     6601 aatttaacgg gtacaaatgg cacatattgc cgcatccgtt aaagataatg ttaaagctgt
     6661 ccgggttacc cggcagtgat catcgtatta aaaagaaaga aggaacacac atggaatacc
     6721 agttcaggga cattgagaaa aaatggcagg caaaatggaa ggcagataac gcttaccagg
     6781 tgaacaatga tttcagcaaa cccaaatttt atgtactgga tatgtttccc tatcccagcg
     6841 gggccggtct gcatgtgggt cacccattgg gctatatcgc ttcggatatc tacagccgtt
     6901 ataaaaggct gaaaggattc aatgtcctgc acccgatggg cttcgacagt ttcggactgc
     6961 cggccgaaca atatgcactc gataccggtc agcatccggc cgtaaccacc aagaagaata
     7021 ttgaaacctt caaaaaacag ctcgataata tcggtttctg tttcgactgg gagcgggaag
     7081 tacagaccag cgatccttct tattataagt ggacacagtg gatcttcctg caattgttcg
     7141 ggtccttctt caaccggcag actgccaaag cggaatcaat cgacagcctg gtggccgttt
     7201 ttgaaaaaga aggtaacagt gctcatgctt gtccgggtga tgaaaagata aaatttacag
     7261 cggctgaatg gaagtccttt gacgaaaaac aacagcagga tatcctgatg caataccgcc
     7321 tggccttttg tggctatggc gaagtaaact ggtgcgaagc cctcggtacc gtgctcgcca
     7381 acgatgaagt ggtgaatggc gtgagcgaac gcggcggtca cccggtagtg aaaaagaaac
     7441 tccgccagtg gtacctgcgc attacagagt atgctgaccg cctgctcgaa ggactggagc
     7501 ggatcgaatt cagtgatgcg atgaaggaaa tgcagacgaa ctggattgga aggtcgagtg
     7561 gggcggagat tgattttgaa gtcaaaaagc cccctctgtc tccccctaag ggggaggaaa
     7621 cggcaccccg ttaccaaacc gcaacctcgt cactgtatag tataaccaaa tctttggcag
     7681 aagaaaaaag gaagaaccca acatcagaag aacagattct ctggcaatta ttaagagcaa
     7741 ataaaactgg ttttcatatt cgccggcaac atattattgg gtcatatatt gtagactttg
     7801 cttgtttgga caaaatggtc actattgaaa ttgatggggg ctaccataat aatcctgaga
     7861 ttcaatttta tgataaagaa agaacccgtt tcttaacagg ccaaggcttt acagaagtta
     7921 gattcacaaa tgaagatgta aacagggacc cagagacagt tgtaaaaaag attactgatt
     7981 tactgtccaa tactgtttcg gttcttccca aaacagggtc ttcggaagtc tcccctttag
     8041 ggggagattt agagggggct ttaaaactca gggtatacac tacaaggccc gatacaatct
     8101 ttggtgttga ctttatggtg gtggcacctg aactggatat agtggaggaa ctgaaatcga
     8161 ctgaacaggc agcagctgtg gatgaatacc tggcttatgt aaagagccgc agtgaacgtg
     8221 aacgcatggc cgagaaaaag atcagcgggg tgtttaccgg cgcttatgcc atcaatccct
     8281 ttgatggccg cgaaattcct atctggatct ctgagtatgt gcttgccggt tatggtaccg
     8341 gggccatcat ggccgtgccc tgcggggatg aacgggatca taagtttgcc cgtcatttca
     8401 atattcccat taccaatatc atcggagctt attacaatgg cgaggaagcc aacccgacca
     8461 aggaagcgac cctggagaat tccggtttcc tgaacggact cctgatgaag gacgcgatcg
     8521 aagtggccgt caccgccatt gaggaaaaag gaatcgggac acgcaagaag aattataaaa
     8581 tgcgggatgc cgctttcagc cgccagcgtt actggggcga acccttcccg attgtctgga
     8641 agaacggaat agcttatccc ttatcagtag atcaattacc attagagtta cccgtagtag
     8701 ataaatacgg acctggcccc gatggagagg gaccactggc taacattaaa gaatggttcg
     8761 gtcagtttga gtctgagatc ccccctttgg ggggcggggg ggccttggag accaacacca
     8821 tgcccggtta cgccggctcc agctggtact ttctccgcta tatggatccg aaaaatgata
     8881 caaacttctg cgaccggaaa gtctccgatt actggaacca ggtggattta tacataggag
     8941 gtaccgaaca cgccgtaggg cacttgttgt atagccgcat gtggaccaag gcacttttcg
     9001 atctcggcca tatcgggtat gatgagccgt ataagaaact ggtgaaccag gggatgatac
     9061 aaggcagcag taggtttgtg tatcgtattg caggcaccaa tcagtttgta tcggcaggat
     9121 taaaagacca atatgaaaca aatgcgatac ataccgatgt aaattttgtt gatggttttg
     9181 aactggatat cgaagccttc aaaaaatgga gagaagagta tacagatgct gaatttattc
     9241 tggaagatgg aaaatatatc tgcggttctg aagtagagaa aatgtccaag tcaaagtaca
     9301 acactgtcaa cccggatgac ctggtctata aatacggcgc tgacacgttc cgcatgtacg
     9361 aaatgtttct cggtccggta gaactcagca aaccctggga taccaagggg attgaagg
//
LOCUS       DAOPMW010000093         9362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8035_length_9362_cov_9.268078, whole genome shotgun sequence.
ACCESSION   DAOPMW010000093 DAOPMW010000000
VERSION     DAOPMW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8035_length_9362_cov_9.268078"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..877)
                     /locus_tag="PLU11_06365"
     CDS             complement(335..877)
                     /locus_tag="PLU11_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPN58775.1"
                     /translation="MIIDIIFGILLVLAIIKGFQRGLIVGVFSMVALLVGLAAALKLS
                     TVVAGYIGNSVNVSSEWLPLVSFLVVFIIVVLLIRLGAKAIESAVEVVMLGWLNRLGG
                     ILLYIAMYTLVYSVFLFYAGKMELLQPETISKSVTYEWIQPLGPKVIGWIGQVLPWFK
                     DMFAELTAFFEKLSHEVPTH"
     gene            complement(933..1385)
                     /locus_tag="PLU11_06370"
     CDS             complement(933..1385)
                     /locus_tag="PLU11_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPN58776.1"
                     /translation="MSLEQKIMGDLKAAMLAKDEAALRSLRAIKASILLAKTAEGSNG
                     EISEDDEIKLLQKMVKQRKDSLEIFTQQNRPELAKKEEEEIAVIEKFLPKQMSADEIK
                     AELEKIITATGAASPADMGKVMGVATKQLAGKADGKTISALVKELLAK"
     gene            complement(1422..1769)
                     /gene="gldC"
                     /locus_tag="PLU11_06375"
     CDS             complement(1422..1769)
                     /gene="gldC"
                     /locus_tag="PLU11_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HPN58777.1"
                     /translation="MHKSTITIEVETDDQKIPAGISWSATDTGQENAQKAKAMMIAFW
                     DSAEKAALRIDLWTKDMMVDEMADFFYQTLMTMADTYGRATRYADQVEEMKTFAKGFY
                     EKFREKQLKENKA"
     gene            complement(1803..2927)
                     /locus_tag="PLU11_06380"
     CDS             complement(1803..2927)
                     /locus_tag="PLU11_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN58778.1"
                     /translation="MRIAVNTRFLLKEYLEGYGYFIQEIFSRLVKAHPEHEFIFLFDR
                     PYDPKFIFGKNVTPVVVGPAARHPLLWKYWYDFKIPAVLKKYKADVFFSPDGFCSLRT
                     RVPQCLVMHDLAFLHFPKGISGPQLRFYKRNTPDYIAKAKTLITVSAFSKADIIEHYP
                     AASNKIRVIHNGIRSSFHPRSFEERETVKQQYTGGKEYFLCTGSLHPRKNGMNLLKAF
                     SLFKKRQQSSMKLVLAGRLAWKYESFTESLKSYKYRDDVILTGYLPEEELAGLTAAAY
                     AMVYPSLYEGFGLPVAEAMQSGVAVITSSNSATQEIAGEAALYADPTDINAIADQLKR
                     IYKDETLRSELAVKGLEQSKAFSWDTAAAQCWKAIEDTIA"
     gene            3208..4416
                     /locus_tag="PLU11_06385"
     CDS             3208..4416
                     /locus_tag="PLU11_06385"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN58779.1"
                     /translation="MKQIYLKSLLLSGFVLLQLVFALPGRSQVIPCPYNFAPGTTVFD
                     TVINTPPGINTLEIKVPQADPYAGMVTCLRVCVSITGVVDSVSVENNSFSSQTANVYY
                     IRTDQITGPGLSSPLTNSINHHYGPYALGASNGTLGSGPDFVSISRDTLLNAVTLCQT
                     ITNLDSLFQFYGNDSISYFYDITAFTNVTCTGGNYNSTVATSALVRFNFEYCTCPGYI
                     LPLHLRNFEATKAAENKALLTWEGFNDASASYRYVTEMSRNGKDFVPVGTVQKDAGVN
                     DQYQFTYTTKQDESGDFFFRVKQVYANSYTRISEIRKVTLKSSVVPRFIISPNPSTGV
                     VGIKFDNNEGGKHKLEIFNAQGQTVVRKDIVLSANSYQQISTLQSGIYWVKLTDMSSQ
                     LSAVSQLYIK"
     gene            4838..5536
                     /locus_tag="PLU11_06390"
     CDS             4838..5536
                     /locus_tag="PLU11_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPN58780.1"
                     /translation="MEYNTTRNHLTMREYGRHVQRMIEHVQTIEDPDRRQANAYAVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLIADFKLDVKSPYPIPTRETLKARPDPLPYPKRN
                     PKFYHLGKNLEEIINKALVEENPDKRLGFANAIAYYMKLAYNNWHKEIVHDDAIQSEL
                     TGITQGQLTFTNTPYVKAYRMQPEGRDNRGNYGKRNNGGGGGKFQQRGGGNGPRRDNR
                     GGSGGGGNFKKKRY"
     gene            6095..7402
                     /gene="murA"
                     /locus_tag="PLU11_06395"
     CDS             6095..7402
                     /gene="murA"
                     /locus_tag="PLU11_06395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPN58781.1"
                     /translation="MYSFEVRGGKKLSGDITPQGAKNEALQIISAVLLTPEKVIVSNI
                     PDIIDVNLLIELLGEMNVKVERPQRDTCIFQADAVNIDYLHSEAYKKKSGRLRGSVML
                     AGPLLARYRRAFIPKPGGDKIGRRRLDTHIIGFEKLGASFTYHEEDGFFHLEAPHLKG
                     TNLLLDEPSVTGTANIVMAASMAVGTTTIYNAACEPYIQQLCRMLNRMGAKISGIGSN
                     LLTIEGVTYLGGTEHRMLPDMIEVGSFIGLAAMTQSDITIRNAGVEHLGIIPEKFRQL
                     GIQLNINGDDIHIPSQEKYEIQTFLDGSVMTIYDHPWPGFTPDLLSIVLVTAIQASGS
                     VLIHQKMFESRLFFVDKLIDMGARIVLCDPHRAVVIGLEREQSLRGITMSSPDIRAGV
                     ALLIAALSAEGKSVIQNIEQIDRGYQDIDGRLRALGADITRIQ"
     gene            7657..8256
                     /locus_tag="PLU11_06400"
     CDS             7657..8256
                     /locus_tag="PLU11_06400"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPN58782.1"
                     /translation="MQIDIFTTIPVLQNDRVLLRPITMDDYDHLLPFSLQEPEIWKYG
                     LVSAAGEENLRSYLAAAVKNRVERKEFAFIVYDKGAGRYAGSTRFYDIQQPWLTTQLG
                     YTWYGKDFQRTGLNRHCKFLLLSYVFDTWGLERCEFRADVKNAPSVAAMKAIGCVEEG
                     ILRNHMPTVQGGRRDSIILSILKTEWEAGGRERLLDKIR"
     gene            8610..>9362
                     /locus_tag="PLU11_06405"
     CDS             8610..>9362
                     /locus_tag="PLU11_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HPN58783.1"
                     /translation="MEKVQLKPLIHREQEWIGIYYSPSNIINGLLKLKAGARWTQTHK
                     CWYVPLNKAAYNKVWFALEGKAELEIQELLQYLEAKKKTDGAFPQEPDLLPVKEPEII
                     SPEEIKQPVPAGLVQKVNNGVLPYLKQELLLRGYSPSTIRTYMNEMRVFLIAIGNHPA
                     GEYTPQQLRDYLQYCYETLKLSEHTLHSRINALKFYFEQVLKREKFFWNIPRPQKPLQ
                     LPKLLNETELTRLFNSLSNKKHKAMLFTAYSSG"
BASE COUNT         2448 a         2078 c         2306 g         2530 t
ORIGIN      
        1 tgtactgacg gaagtattcg atcaggcttc tgaaattgct gagcgccccg aaaagtccat
       61 gcagcagcaa caacggttcc ccgctgcctt cctcgatgaa cttaaacttt tcctcttgtt
      121 tgatttcgta atccatgggc agtcaatctg agctttaacg ctggttgcta aaataaacgt
      181 tttgcggtaa aattcaggtc tttgtggcac aaaaccaacc tgatttgcgc aaatataggg
      241 gctgcgggtc tgttttattc aatggagata aaaatcagcg gcaccgcgcc tgtaaggtat
      301 tgtaaaaaag ccgggaaaac tatgatcaac ccaatcaatg tgttggcacc tcgtgggata
      361 gtttttcaaa gaaagcggtc agctcagcaa acatatcctt aaaccaggga agaacctggc
      421 cgatccaacc gattactttc ggtcccaggg gctgtatcca ttcgtaggtt acggatttac
      481 tgatagtttc cggttgcagg agttccattt tacccgcgta aaataagaac acactgtata
      541 ccagtgtata catggcgata tataaaagaa ttccgcccag ccggttgagc cagcccagca
      601 tcaccacttc cacggctgat tcaatggctt tggcaccgag tcttatcagc agtactacaa
      661 tgataaacac gaccaggaat gaaacaagcg ggagccattc cgacgataca ttcaccgaat
      721 taccaatata gccggccacc acggtggata gtttgagtgc tgcggcgagt cccaccagca
      781 gggctaccat tgaaaaaacg ccaacaatca atccccgctg aaagccttta ataatggcca
      841 gcaccagcag gatgccgaaa atgatatcaa taatcatatg gctgtaacgt tgaatggccc
      901 cggttccgta ccggccagct gttaaaatcc gtttatttcg cgagcagttc ctttaccagg
      961 gcggagatgg ttttgccatc ggctttgccg gccagttgtt tggtggccac gcccattact
     1021 ttccccatat cggcgggaga agccgcgccg gtagcggtga taattttctc cagttccgct
     1081 ttgatttcat ccgccgacat ttgtttcggc agaaattttt caatcactgc gatttcctct
     1141 tcttcttttt ttgccagttc ggggcggttt tgctgcgtaa agatttcgag agaatcttta
     1201 cgttgcttca ccattttctg taaaagtttg atctcatcgt cttcggaaat ttcgccgtta
     1261 cttccttcgg ccgttttggc cagcaggatc gaggctttaa tagcgcggag actgcggagg
     1321 gcggcttcat ctttggccag catggcggct ttcagatccc ccataatttt ttgttctaaa
     1381 ctcatagttg tttatttgag tagttaagaa gttataaccg gtcaggcttt gttttccttc
     1441 agttgttttt ccctgaattt ctcataaaac cctttggcaa aggtcttcat ttcctctacc
     1501 tggtcggcat agcgggtggc ccggccatag gtatcggcca tagtcatcag ggtctggtaa
     1561 aagaaatcgg ccatttcatc caccatcatg tctttggtcc agagatcgat ccggagggcg
     1621 gctttttcag cgctgtccca gaaggcgatc atcatggcct tggccttttg tgcattttcc
     1681 tgcccggtat cggtggcgct ccaactgatt ccggccggaa ttttctgatc gtctgtttct
     1741 acttcaatag ttatcgttga tttgtgcata tacttgtttt gagagggcaa aggtaaacga
     1801 aatcaggcga tggtatcttc aatggctttc cagcattggg cagcggcagt atcccagctg
     1861 aatgctttgg actgctccag gcctttcact gccagttcgc tccgcagggt ttcatccttg
     1921 tagattcgtt tcagctgatc cgcaatcgca ttgatatccg tcggatcggc atagagagcg
     1981 gcctccccgg ctatttcctg cgtggctgaa ttggatgatg tgatcacggc tacaccggac
     2041 tgcatggctt cggcaacggg taaaccgaag ccttcataga gtgaggggta taccatggca
     2101 taggcagcgg cggtaaggcc ggccagttct tcctccggga gataaccggt caggattaca
     2161 tcgtcccggt acttataaga cttcaggctt tcggtaaagg attcgtattt ccaggccagt
     2221 cgtccggcca gcacgagttt catggaggat tgctgtcttt ttttaaacag ggaaaaggct
     2281 ttcagcaggt tcattccgtt tttacgtggg tgcagggagc cggtgcagag aaaatattct
     2341 tttccgccgg tgtattgttg tttcaccgtt tccctttctt cgaatgagcg gggatggaaa
     2401 gaggaacgga tgccattgtg gatgaccctg atcttattgc tggcagcggg atagtgttcg
     2461 ataatatccg cttttgaaaa tgcagaaacg gtgatcagcg tttttgcttt ggcgatataa
     2521 tccggggtat tgcgtttata aaaacggagt tgggggcctg aaattccttt gggaaaatgc
     2581 aggaaggcca ggtcatgcat cacgagacat tgtggtaccc gggtgcggag ggagcaaaat
     2641 ccatcagggg agaaaaaaac atccgcttta tattttttca gcacagcggg tattttaaaa
     2701 tcataccagt atttccagag aagcgggtgt cgggcggcgg ggccaaccac cacgggggtg
     2761 acatttttcc cgaaaatgaa tttcggatca tatggccggt caaacaggaa aataaattca
     2821 tgctccgggt gggctttcac cagccggctg aaaatttcct gtataaaata cccgtaacct
     2881 tccaggtatt cttttaataa aaatcgtgta ttgacggcaa ttctcatatc cggacacaaa
     2941 ggtaaccgcc tgaccgggga ttcccgcgta aatcaacgta gtgacttcta aataaacaag
     3001 gaggagaacg tcgtatagtt tcccggaatg tcagtaagta agcgcctgtt ttttacccgg
     3061 ttattttaac cggttttatg agcagattcc cggtgatgtt tcattaaata ccggttttaa
     3121 aaatgagtac attaccaatt gcaaaaccgt gtggcaaatt ctacttttac acagtgcagt
     3181 ttctttttga aaccaaaact ccgacctatg aaacagatct acttaaaatc gcttcttctt
     3241 tccggtttcg ttttactcca gttagttttt gctttaccgg gccgcagcca ggtgattccc
     3301 tgtccttata attttgcacc aggcaccacg gtgtttgata ccgtgatcaa tacgcctccc
     3361 ggcatcaaca cacttgaaat caaagtgccg caggccgatc cttatgccgg gatggtgacc
     3421 tgtttacggg tttgtgtgtc gatcaccggt gtggtggatt ctgtcagtgt ggagaacaat
     3481 tcattttcct cacaaaccgc caatgtatat tatatccgta ccgatcagat cacgggtccc
     3541 ggtctcagtt caccgctgac caatagcatt aatcatcatt atgggccgta tgcgctggga
     3601 gccagtaatg gtaccctggg ttcaggccct gattttgtgt ccatatcaag agatacgctg
     3661 ttgaatgcgg taacgctttg ccagaccatt accaacctgg attcactttt ccagttttat
     3721 ggtaacgaca gtatttccta tttctatgat ataaccgcct ttaccaatgt aacctgtaca
     3781 ggagggaatt ataacagtac cgtagccaca tcggccctgg tgcggttcaa ttttgagtat
     3841 tgtacctgtc ccggttatat cctgcctttg cacctgcgca attttgaggc tacgaaagcg
     3901 gctgagaaca aggccttgct gacctgggaa ggattcaatg atgcttcggc ttcttaccgt
     3961 tatgtgacag agatgagcag aaatggcaaa gattttgtac cggtagggac cgtgcagaaa
     4021 gacgcaggcg taaatgatca gtatcaattt acttatacga cgaagcagga tgaaagtggt
     4081 gattttttct ttcgtgtgaa acaggtgtat gccaatagtt acacacgaat cagtgagatc
     4141 agaaaagtta cgttaaaaag ttccgtggta ccccggttta ttatcagtcc taacccatca
     4201 acaggtgtag ttggtatcaa atttgataat aatgagggcg gcaaacacaa actcgaaata
     4261 ttcaatgcac aggggcaaac ggtggtccgc aaggacattg tgttatcggc taattcgtat
     4321 cagcagatct ccacgttaca aagcgggatc tactgggtaa aactgaccga tatgagcagc
     4381 cagttgtccg ctgtcagtca actctatatt aaatgacctc ttaggtggaa gaggatggtg
     4441 gcttgtaaaa ggggccttgg ttcggggccc ctttcttttt ttccggtctg ttgattacaa
     4501 atcaggagag attcatactt agaaatgtaa aaggttggag gttggaagtt ggaggttcga
     4561 ggttttcgtg ttaagggaaa aggactttac aatctgcaag tgtacttaaa ctcaactcaa
     4621 tacctgtctg attactgaaa ttttgtgatt atgagttaat ccagaactat taatctttga
     4681 ctttatacag gtataaacgg agtttttctt aaagaatagt tgtttcgcat atcacgataa
     4741 cctcgaacct ccaacttcca acctccaact accaaatcgt cgtttctttc aaatttgagg
     4801 gcaattccct atttttgacg gtaccgaaca aattttcatg gaatataata ctaccagaaa
     4861 tcacctcacg atgcgggaat atggccggca cgtgcagcgg atgattgaac atgtgcagac
     4921 gattgaggat ccggatcgcc gccaggccaa tgcctatgcg gtaattgaac tgatgggttt
     4981 tctgaacccg cacctgaaga atgtggagga tttccggcat aaactctggg atcacctctt
     5041 cctgattgcg gatttcaaac tggatgtgaa atccccctat ccgattccca cccgggaaac
     5101 actgaaggcc agacctgatc ccttgcctta ccccaagaga aatcctaaat tctatcacct
     5161 gggtaaaaac ctggaggaaa ttatcaataa ggccctggtg gaagaaaatc cggacaaacg
     5221 gcttggtttc gccaacgcga tcgcttatta catgaaactg gcctataata actggcataa
     5281 ggagatcgtg catgatgatg ccatccagag cgaactgacc ggcattacgc agggacaact
     5341 gactttcacc aacacgcctt atgtaaaagc ataccgcatg cagcccgaag gacgcgataa
     5401 ccgcggcaat tacggcaaac gtaataatgg cggaggagga ggaaaattcc agcagcgcgg
     5461 tggagggaat ggcccgagaa gggataacag aggtggcagt ggtggtggtg gaaatttcaa
     5521 gaagaaacgt tactaatacg agatattttc ttagaaggct aaccaggtaa aagaggttag
     5581 cctttttttg tgcccgggag ataaatgtgc caatatgcca attagccaat gtgccaatgg
     5641 acctgctaag ggggttgtac taaagccagg actatagctt atttagcaga tcagttaaat
     5701 acaagattcc tccttcgtcg gaattaaagt gcttatcaag agcgtcttcc tgatccctct
     5761 ttcgtcggga tgacaaaggg gcacatggat atttatcaaa ccactattta agatagaaac
     5821 aggaaattct aagaaacctt gtcaccccga cgaaggaggg gtaacggagt cctggagtat
     5881 atagaaattc aattccagag tttaccctga gattgccgaa gggaaggagg aatcttgtat
     5941 taagcaaata ctctaaatac gtaactggac tttggtttag acaaatcctt aaaggaggtt
     6001 aattggcaca ttggctaatt ggcatattgg cacattgttc tgatttttct ctaatttgcc
     6061 cctttcaacc aatcattctt aacaacaata acctatgtat tccttcgaag tcagaggcgg
     6121 caaaaaactc agcggcgaca tcactcccca gggtgccaag aacgaggccc tgcagatcat
     6181 cagtgcggta ttgctgacgc cggaaaaagt gatagtgagc aatattcctg atatcattga
     6241 cgtgaacctg ttgattgaat tactaggtga aatgaatgtg aaggtcgagc ggccgcagcg
     6301 ggatacctgt atttttcaag cagatgccgt taatattgat tacctgcaca gtgaggcgta
     6361 taagaaaaaa agcggaagac ttcgtggctc cgtgatgctg gctggtccgt tgctggcccg
     6421 ttaccgccgg gcttttattc ccaaacccgg gggtgataag atcggtcgcc ggagactgga
     6481 tacccatatt atcggttttg aaaaattagg ggcctcattt acttatcatg aagaagatgg
     6541 cttctttcat ttagaagccc cgcacctgaa aggcacaaac ctgttgctgg atgaaccttc
     6601 cgtaaccggt acggccaata tcgtgatggc cgcttccatg gccgtcggca ccactactat
     6661 ctataatgcg gcctgcgaac cctatatcca gcagctctgc cggatgctta accgcatggg
     6721 ggcgaagatc agcgggatcg gcagtaacct gctgaccatc gaaggggtga cttatctcgg
     6781 aggtaccgaa caccgcatgt tgcccgatat gattgaagtc ggttccttta tcggtctcgc
     6841 cgccatgacg cagagcgata tcactatccg gaatgccggt gtggaacatc ttggtatcat
     6901 tcccgaaaaa ttccgtcagc tgggcattca attaaatatt aacggagacg atatccatat
     6961 tccttcgcag gagaaatatg agatacaaac ttttctcgat ggttcggtga tgacgatcta
     7021 tgatcatccc tggccgggct ttacccctga tcttttgagt attgtactgg ttacggctat
     7081 acaagccagt ggttcggtac tcattcacca gaagatgttt gagagtcgcc tcttttttgt
     7141 cgataagctg atcgatatgg gtgcccgcat cgtgctctgc gatccgcacc gcgccgtagt
     7201 aatcggactg gaacgcgagc aatcactccg cggcatcacg atgagttcgc cggatatacg
     7261 ggccggtgtg gcattgctca ttgcagccct gagtgccgaa ggcaagagtg tgattcagaa
     7321 tattgagcag attgacaggg ggtaccagga tattgatggg agactacggg cgttgggagc
     7381 ggatatcacg agaatacagt agtaagaacc aagatgccaa gataccaaga tgccgaaata
     7441 agcatcaaga aaccaagaca gccaagaagc caaagaaata tcaaattaca agtttcaaat
     7501 aagcatcaat aaaaaagcac taatccttct gtgaactttc gcagtaggat tggtgctttt
     7561 ttggaatttc tcttttggtt cttctttgat ccttttttat tggttcttct ttggcgtctt
     7621 ggtatcttag gttcttggtt cttttaaaca atacacatgc aaatagatat tttcaccacc
     7681 attcctgttt tacaaaacga ccgcgttctc ctccgtccca ttactatgga tgattatgat
     7741 catctgctgc ccttttccct gcaggagccg gagatctgga agtacggttt ggtttcggct
     7801 gcgggggaag agaacctgcg gagttacctg gcggcggcgg taaagaaccg ggtggagcgg
     7861 aaggagtttg cttttatcgt gtatgataaa ggggcgggtc ggtatgccgg gagtactcgc
     7921 ttttatgata ttcagcagcc ttggctgact acgcagctgg ggtatacctg gtacgggaag
     7981 gattttcagc ggacgggact gaaccggcat tgcaagtttc ttttgctttc gtatgtgttt
     8041 gacacctggg gactggagcg ttgtgagttc cgggctgatg tcaaaaatgc gccgagtgtg
     8101 gcggccatga aggccattgg ttgtgtggaa gagggcattt tgcgcaatca tatgcctaca
     8161 gttcaggggg gccggcggga cagtatcatc ctcagcatcc tgaaaacgga atgggaagcc
     8221 ggagggcgtg aaaggctgct ggataaaata cgttagtgta ttattctccc ccttgttgcc
     8281 ttcatacaga cacctgtaaa accaataaaa ttttaacaga attacctctc ttgtcagatc
     8341 cggattcagg aaaataattt atatttaatt agccaacctg gtccttaaaa ttcaatcatt
     8401 tcctttctta agggctggta tgtgttgcag gtaaaccggc tgtgtttttc ccaccgattt
     8461 atctgaatat cagggcactt ataataccgg ccggccgttg ctgctgtgac tgaggtgcac
     8521 gaagtgcgtt ccgaaaaaaa acattttttc cggttaaaaa caccgttttg tttcagggag
     8581 tatggataag tttccggtaa actttatcca tggaaaaagt acaactgaag ccgctcattc
     8641 accgggagca ggaatggatc ggtatttatt attcgccgtc caatattatc aatggtttat
     8701 tgaaattgaa agccggggcg cgctggacac agacccataa gtgctggtat gtgccgctga
     8761 ataaggcggc ttacaataaa gtgtggtttg ccctggaagg aaaagcggag ctggaaatac
     8821 aggaattgct gcagtacctg gaagcgaaaa agaaaactga tggtgctttt ccacaggagc
     8881 ccgacctcct gccggtgaaa gaaccagaaa taatatctcc tgaggaaatt aaacaaccgg
     8941 taccagcggg tctggtacag aaagtgaata atggggtact cccctatctg aaacaggaac
     9001 tgctattgag agggtacagt ccttccacga tcaggaccta tatgaacgaa atgcgggtgt
     9061 tcctgatcgc gatcggcaat catccggcag gggagtatac gccgcagcaa ttgcgggatt
     9121 atctgcaata ctgttatgaa acactgaagc tgagtgagca taccctgcac agccggatca
     9181 atgcgttgaa gttttatttt gagcaggtgc tgaagcggga gaaattcttc tggaatattc
     9241 cgcggccgca gaagcccttg cagttgccga agttgttaaa tgaaacagag ctcacgcggt
     9301 tgttcaattc gttgagcaac aagaagcata aggccatgtt gtttacggcc tacagttcgg
     9361 gt
//
LOCUS       DAOPMW010000094         9361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8037_length_9361_cov_10.752525, whole genome shotgun sequence.
ACCESSION   DAOPMW010000094 DAOPMW010000000
VERSION     DAOPMW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_9361_cov_10.752525"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3487)
                     /locus_tag="PLU11_06410"
     CDS             complement(<1..3487)
                     /locus_tag="PLU11_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58784.1"
                     /translation="MLKKQKQKQNMKRNLTQFAVKAAGTAVLLAILFIGITLQAMAEG
                     SKDLYPSGMTTGRRAYLISRPLATDPAAYFPFPTPGTTRVYAKVGETIYAGFSQQGKS
                     YNGMVAVANITMRAPNGNTYSSPTNSGNTTGLIANRTEELNGPDRGSITAGYTPFYRT
                     VLSGEEGIWEVDFNAMGTGTNFTKGPNYSNDGKWVGEYNPDEAWFQPTSGGDPSTANN
                     HSSALIAAWDVSVGSVSDRAILIPGRVFTTVLALCLPNNFPGAGSAMGGTNGSGTTII
                     SGSGGFYSTFYVLTNDGYAYKVNNHGINGASWSFFANNKGIVSGGTQLPGTNGFTGGE
                     PTYQSQNSIGSTTQNTHDPRFLDATNDFTHKVFYSKPASDLPASATAIYRRKDGSNTI
                     TTTWLKTSALNPEVSDVSIKGVEANTDWVVGPDGAYISFTSNVYGSYVIDIDLNGNGQ
                     FDDPADRRWTGSAVIGTNTVTWDGKDGLGNLITIGTTEVTVRAQVNLGEVHFPFVDGE
                     INPNGVIFERLDESYNVIPGQDLLYWDDSNITITEGCDPNRYTASNPLKNVYTGLSSN
                     SNGHKWGSTVTSAPFEGGCGSAQDVNDSNGKQDFGNGRILDSWTYVGGAQQITRDIFS
                     KYLDLAVSAPDPSPIAVCENGTVTYSVNVTNASGTNGSDAAEGAKFRFDAPAGLTIVS
                     RTVTVNFGTFAETFSSGLGTSQYNAVFNQSNPGSVTYSFTCVVTGTITNPTAYVMRPP
                     DVFDTDATSSVSGAPADYQVECDGGTSGTGCNNIRTATTSVIILSSGVDTDNDGIDDI
                     CDLDDDNDGILDIDEALCLPDGTAFSINSTRVPTPYALNTALQGGSTGTANLTGFAYG
                     TFNFTASVSGTATWGDGVQIQNNVTVGDHIYLQPQNAPGGGAANFATYTIDFPYPVLD
                     FSFVSAGLNYNDFYEITAFYNNTPITVNSSNFSGFNPDLASGSWTINGSRISNSSTAG
                     GTDVTENVFTTSFSGPVDKVVIKSGKIDGLTNTITTAITSMEFCSLSRDTDKDGIFDH
                     LDNDSDNDGCPDAIEGAGSYTAADLDDNNRLDYANLSPSGVDGDGVVNAGSQATAPAV
                     ITSDVLTVPVITQNPSPVCAGETITFTATTSGVRVTNFGTTGSTDDDTTIPIPTGDII
                     YKWYLGTTLITDGGIY"
     gene            complement(3524..3712)
                     /locus_tag="PLU11_06415"
     CDS             complement(3524..3712)
                     /locus_tag="PLU11_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58785.1"
                     /translation="MKEAFLKRSDGLNEFSALASLAVGLMFFILDTSQWRNSKRSRNI
                     NPGKTCLLPGVNEQHFQV"
     gene            complement(3847..4200)
                     /locus_tag="PLU11_06420"
     CDS             complement(3847..4200)
                     /locus_tag="PLU11_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPN58786.1"
                     /translation="MINAIYIMKVDSGNKESLESILKLVISRTKYLPGCAKAEIWYND
                     ETSEIMVKEIWRKDYDLDRHINSPLFKKMLAALELSADKPGIQFCDCQHMRGLDFIEE
                     VIKQNQGIVPVFPEQ"
     gene            4655..6670
                     /locus_tag="PLU11_06425"
     CDS             4655..6670
                     /locus_tag="PLU11_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN58787.1"
                     /translation="MKNFFPFFLCVTLNIVTFAQSDTWSVKFSNAIISRYQPTINAMT
                     NKGWEYSNGSILHGMEKVYLNTHDADYLNYIKAYVDSYVDASGNVTGLGMTVDKIQPG
                     VLCLFLYEQTGQTKYKTAATQIKNYVNNFYKTPDGGIWHKSEQNVTSYKYYNVMMVDG
                     MYMLHPFLTKYASMFNEPALFDAATFQLLFLASKVMPQPMTLPKHAWEYYDYKAWSDP
                     STHMSTDVWSRGTGWYMMALADVLEYLPTSHANYGAILELFQRMSSGVAANQHPTTGL
                     WYQVVDKRTETGNWTESSGSGMFVYALKKGVEKGWLNAATYNPVINLGWQGLQTQIAI
                     LGDSGPQIKQFCVATGVVDNTAAYYALGKTDCPTAYPWSGTQHPHGYCGILMAASVME
                     FPLVNSLPSVSITSPSNGTSYTEPASVIITATASDTDGTISKVEFYQGTTKLGEDTSS
                     PYTVTWSGVTQGTYNLTVVATDNSGGIATSTAVTITVNPAISSQTFSARISDGMNDAE
                     EFSNGTVTRTSYALEMAFYSSIAGTQVVGLRFINVTIPKNATITQAYLQFTASTNNYA
                     IPNLTIRGENSGNSAAFALTSGNISARTLTSSSVTWIPPSWAVIDESGSAQKTSDLSA
                     IVQEIVNRPDWDSGNSMTFIISGIGARTAHSYETSSDKSAQILIDYY"
     gene            6751..>9361
                     /locus_tag="PLU11_06430"
     CDS             6751..>9361
                     /locus_tag="PLU11_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPN58788.1"
                     /translation="MKNFFPFFLCVTLNIVTFAQSDTWSVKFSNAIISRYQPTINAMT
                     NKGWEYSNGSILHGMEKVYLNTHDADYLNYIKAYVDSYVDASGNVTGLGMTVDKIQPG
                     VLCLFLYEQTGQTKYKTAATQIKNYVNNFYKTPDGGIWHKSEQNVTSYKYYNVMMVDG
                     MYMLHPFLTKYASMFNEPALFDAATFQLLFLASKVMPQPMTLPKHAWEYYDYKAWSDP
                     STHMSTDVWSRGTGWYMMALADVLEYLPTSHANYGAILELFQRMSSGVAANQHPTTGL
                     WYQVVDKRTETGNWTESSGSGMFVYALKKGVEKGWLNAATYNPVINLGWQGLQTQIAI
                     LGDSGPQIKQFCVATGVVDNTAAYYALGKTDCPTAYPWSGTQHPHGYCGILMAASVME
                     FNTINVPATGVAVSPASLTLSVGNTQQLTATVAPSNATNKSVTWSSSNTSVATVSPTG
                     LVTAVVPGNAVITTTTQDGNFTSSSSVTVNAVIPCTTIQYQAESGTFTSGTTIDTNWA
                     GYTGTGFINTPNATGSYLQITVTALIAGTHDFTIRFANGTTTDRTASVAMNGSTVLPS
                     LSMAATGSWSTWSTSAFSVPLVAGTNTIRFTALTTGGLANIDRIDMCQKYMPVTGVTV
                     TPASATLAPGGTQQLTVNVEPTDAINKVVSWSSSNTSVATVSSTGLVTAVAAGNAAIT
                     ATTQDGSFTATCQITVSAVSVTGITVSPSSATLEIGETTTLIAAVFPVNATNKVVSWS
                     SSNTSVATVSSTGLVTAVAAGNAAITATTQDGSFTATCQITVSAVSVTGITVSPSSAT
                     LEIGETTTLIAAVLPANATNKVVSWSSSNTSVATVSSAGLVTAVASGNAVIIVTTQDG
                     NFTSSSSVTVNAVI"
BASE COUNT         2610 a         2304 c         1944 g         2503 t
ORIGIN      
        1 tataaatacc accatcggta atcagtgtag tgccaagata ccatttatat ataatatcgc
       61 cagtaggtat aggtatcgtt gtatcatcgt ccgtagaacc tgttgttccg aagttggtaa
      121 cgcgaactcc cgatgtagtt gcagtaaagg ttatagtttc acctgcacaa acaggggatg
      181 gattctgtgt aatcaccggt actgtcagaa catcgctggt gataactgct ggtgcagtgg
      241 cttgagaacc tgcatttact actccgtcgc catccactcc cgatggggat aaattcgcat
      301 aatccaatct gttattgtca tccagatcgg ccgcagtata gctgccagct ccttcaatgg
      361 catccggaca accatcgtta tcgctgtcat tatccagatg gtcaaatatc ccatccttat
      421 ccgtatcccg tgataatgaa caaaattcca tagaagtaat agcagtagtt attgtattag
      481 ttaaaccatc aattttccct gattttatca ctactttatc aaccggacct gaaaacgatg
      541 ttgtaaatac attctcagta acatccgttc ccccggccgt acttgaattg gaaatacgac
      601 taccattaat cgtccaactt ccgctggcca gatccggatt aaaaccactg aaattactgc
      661 tgtttacagt aattggcgta ttattataaa aagccgtaat ttcataaaaa tcgttgtaat
      721 taagacctgc cgaaacaaag gaaaagtcca atacaggata tgggaaatcg atggtataag
      781 tagcaaaatt ggcagcacca ccgccaggag cattctgagg ttgtaagtaa atatgatcac
      841 caacagtcac attattctgg atttgaactc catcgcccca ggtggcagta cctgaaaccg
      901 atgctgtaaa gttgaaggtt ccataagcaa aaccggtcag attcgctgta ccggttgatc
      961 ctccctgcaa cgctgtattt agggcatacg gtgtcggaac acgtgtggaa ttaatggaaa
     1021 aagcagtccc gtcaggcaaa caaagtgctt catcaatgtc caaaattccg tcattatcat
     1081 catcaaggtc acaaatgtcg tctataccat cattgtcagt atcaactccg gaagatagaa
     1141 taataactga tgtagtggca gttcttatgt tgttacaacc cgttccgctg gttcctccgt
     1201 cacattcaac ctggtaatcg gcaggtgcgc ccgatactga agaagttgca tccgtatcaa
     1261 aaacatccgg aggacgcatt acataagcag tcggattggt aattgtcccg gttactacac
     1321 atgtaaagga ataggtcacc gaacctggat tggactggtt aaaaacagca ttgtattgcg
     1381 atgtccccag cccgcttgaa aaagtttcag caaaggttcc aaaattaaca gtaaccgttc
     1441 tcgacacgat ggtcaatcct gcaggagcat caaagcgaaa tttcgcacct tcagctgcat
     1501 cagaaccgtt ggtccctgaa gcattggtaa cattgaccga ataagttacg gtaccgtttt
     1561 cacaaacagc aatcggactg ggatcagggg cagaaacagc cagatctaaa tatttgctaa
     1621 aaatatcccg ggtaatttgc tgagcacctc cgacataggt ccaggaatct aaaatcctgc
     1681 catttccgaa atcctgtttt ccattactgt cgttaacgtc ctgcgcagaa ccacatcccc
     1741 cctcaaatgg agcacttgtt actgtactgc cccacttatg cccgtttgaa ttgcttgata
     1801 aaccggtata cacatttttt agaggattgg aggccgtata tctattagga tcacatcctt
     1861 ctgtaatggt tatatttgaa tcatcccaat ataacaaatc ctgccctgga atgacattgt
     1921 aactttcatc caggcgttca aaaattaccc cattaggatt tatttctcca tcaacaaacg
     1981 gaaaatgaac ctccccgaga ttaacctgag cccgaaccgt gacctcagtt gttccaattg
     2041 tgatcaaatt gcctaaacca tcttttccat cccacgtaac tgtgttggtt ccaattactg
     2101 ccgatcccgt ccaccttcta tcagccggat catcgaattg tccatttcca ttgaggtcaa
     2161 tatctattac ataacttccg tacacattag acgtgaatga tatgtaagct ccatcaggtc
     2221 ccacaaccca gtctgtatta gcttcgacac cttttatact tacatctgaa acttcgggat
     2281 taagggcaga ggtcttcagc catgttgttg taattgtgtt tgacccatct tttctacggt
     2341 aaattgcggt tgcagatgca ggtaaatcac ttgcaggttt gctgtaaaaa actttatggg
     2401 taaaatcatt tgtggcatcc aggaaccttg gatcgtgggt attctgcgtt gtacttccaa
     2461 tagaattctg actttggtat gttggttcac ctccggtgaa tccatttgtt ccgggtaact
     2521 gagtccctcc tgaaacaatt cctttattat tggcaaaaaa cgaccaggaa gcaccattta
     2581 ttccatggtt attcacttta taagcatacc cgtcattagt caaaacataa aaggtagaat
     2641 aaaaacctcc acttccactg ataatggtgg taccagaacc attggttccc cccatggcac
     2701 ttcctgctcc cgggaagtta ttaggaagac acaatgctag tacagttgtg aaaacacgac
     2761 cgggtataag tattgcacga tctgacacag aacctacaga tacatcccag gctgcaatga
     2821 gtgcgctgga atggttattc gcagttgatg ggtctcctcc cgaagtaggc tggaaccaag
     2881 cttcatcagg attgtactca ccgacccact tcccgtcatt actataatta gggccttttg
     2941 tgaagttagt ccctgtcccc atggcattaa aatcaacttc ccagattcct tcctcgccag
     3001 ataagacagt acgataaaac ggagtatatc cagccgtaat cgaacccctg tcgggaccat
     3061 tcaattcctc ggtacggttt gctatgagac cggttgtatt tcctgagtta gtcggggaag
     3121 aatatgtatt tccattggga gcccgcatcg ttatattggc aactgcaacc ataccattat
     3181 aactctttcc ctgttgactg aaaccggcat aaatcgtttc tccgactttc gcatacactc
     3241 gtgttgttcc tggggtcggg aaagggaaat atgccgccgg gtcggttgcc aacggtctgc
     3301 ttattaagta agccctcctg cctgttgtca tacctgaagg atacaaatcc ttactccctt
     3361 ctgccattgc ctgcagggta attccgataa aaaggattgc cagtaaaact gcagtaccgg
     3421 cagcttttac agcaaattgc gttaaattcc ttttcatatt ttgcttttgt ttttgctttt
     3481 ttaacatggg taagaaatgt gggaaatttt cgaatgggat gaattatact tggaaatgtt
     3541 gttcgttaac acccggaagc aggcaggttt tgccaggatt gatattcctg cttctcttag
     3601 aatttcgcca ttggctggta tctaaaataa aaaacatcaa ccccacagct aatgaggcca
     3661 atgctgaaaa ttcgttcaga ccatctgatc ttttcaggaa ggcttctttc atataggctt
     3721 accaggaagt aaaaattatg tcaataatta taatttattt ttgtttcact tattttctgc
     3781 ggcaattata acgataattt acatttgtat caatattcaa tacccaaatc ccttatctaa
     3841 tcaacttcac tgttcaggaa aaacggggac tattccctga ttttgtttga taacctcttc
     3901 gataaaatca agaccccgca tatgctgaca atcacaaaac tggatgcccg gcttatcggc
     3961 gcttaattcc aaagctgcca gcatcttttt gaacaacgga gagttgatat gcctatctag
     4021 atcatagtcc ttgcgccaga tttcttttac cattatttca gaagtttcgt cattgtacca
     4081 aatctccgct ttggcacaac ccggcaaata ctttgtccgg gaaataacca attttaatat
     4141 actctccagg ctttctttat ttccggaatc gaccttcatt atataaattg cgttgatcat
     4201 actgtaaatt ggacatattc aatgctcaat tttaagtcca aaaacagaat tgtcataggt
     4261 gttttgagga tttttacaga ttataagatt gataatgtga tatctaatgt cttatttttt
     4321 taaaacatag aaagtaacac tgcgcaaaaa atgcatttta tgcgcaatct attgcgcatt
     4381 ttctaaattc atccttaacc ataacaaacg attccagccc tggccgggat tcttagtaaa
     4441 ttataagaaa atcattgttg aattttagaa caactttctt taattttcgt attttgcaac
     4501 atatttatct tttgggctcg taataaaaca aatctgatga gaaaacaaat taccattgtt
     4561 tatcgattat catatatttg ttaagatgaa acttttataa caaaccaaaa ctgattttca
     4621 gtttcgcatg tccaataatt aaaacaaacc ccgtatgaaa aatttctttc cgtttttttt
     4681 atgtgttaca ttaaacattg taaccttcgc gcagtcggat acctggtcgg tcaaattttc
     4741 caatgccatt atcagccggt atcagcctac catcaatgcc atgaccaata agggatggga
     4801 atactctaac gggagcattc tgcatggtat ggaaaaggta tatctgaata ctcacgatgc
     4861 agactacctg aactatatca aagcttatgt cgacagctat gtggatgcca gcggaaacgt
     4921 cacgggtctc gggatgaccg tagacaaaat ccaaccgggg gtgttatgtc ttttcctgta
     4981 tgaacagacc ggccaaacca aatacaagac cgctgccacc cagattaaaa attacgtcaa
     5041 caatttttac aaaacccctg acggcggaat ctggcataaa agtgaacaaa atgtaacttc
     5101 ttacaagtat tacaacgtca tgatggttga cggaatgtac atgctgcacc cgttcctcac
     5161 caaatatgcc agtatgttca acgaaccggc actgtttgat gcagcgactt ttcagctcct
     5221 cttcctggca agcaaagtta tgccccagcc tatgacgctt cccaaacatg cctgggaata
     5281 ttacgactac aaagcatggt cagatccttc cacccatatg agcaccgacg tatggtcgag
     5341 aggcaccggc tggtatatga tggccctagc tgatgtactc gagtacctcc ccacctctca
     5401 tgccaactat ggcgcaattc tggaactgtt tcagcgcatg tcgtcgggag tggctgccaa
     5461 ccagcatccc accaccggat tgtggtacca ggtggttgac aaaaggacag aaacaggcaa
     5521 ctggacagaa agttcaggct cagggatgtt cgtgtatgcc ctgaaaaagg gagtggagaa
     5581 agggtggttg aatgctgcca cctacaatcc ggtgatcaac ctgggatggc agggtctcca
     5641 aacccaaatt gcaatacttg gcgacagcgg accacagatc aaacagtttt gtgtggctac
     5701 gggagtggtg gacaatactg ccgcctatta tgcactgggc aaaaccgatt gtcccaccgc
     5761 gtatccctgg agcggaaccc agcaccctca cggttactgt ggaattctga tggctgcctc
     5821 tgtcatggaa tttccccttg ttaactcact acccagcgta tccattacaa gcccttcaaa
     5881 cggaacgagc tatacagaac ccgcctctgt gattataacc gccacagcct ccgacaccga
     5941 cgggaccatt tcaaaagtgg aattttacca ggggactacc aaactgggcg aagacacatc
     6001 gagtccatac accgtgacat ggagcggcgt tacacaggga acctacaacc tgacagtcgt
     6061 tgctactgat aattctggtg ggattgccac ttctaccgct gtaactataa ccgtgaatcc
     6121 tgctatctca tcgcagactt tcagtgcgcg aataagtgac gggatgaacg atgctgaaga
     6181 attttcgaat ggcacagtca cccgaaccag ttacgctctt gaaatggcct tttattcttc
     6241 cattgcaggg acccaggttg tcggactacg ctttatcaat gtaactatac ctaagaatgc
     6301 cacaattact caggcttatc tgcagttcac ggcgtcaaca aataattacg ccattcctaa
     6361 tttaaccatc cggggtgaaa attcgggaaa ttccgctgct tttgccctga cgtccggaaa
     6421 tatttccgcc cgtacactca cttcttcttc tgtcacatgg attccacctt cctgggctgt
     6481 cattgatgag tcgggttccg cgcagaaaac ttccgatttg agtgccatag tgcaggaaat
     6541 tgtgaacaga ccggattggg attccggaaa cagtatgaca ttcattatct ccggaatcgg
     6601 cgcccgaact gcccattctt atgaaacatc ttccgacaag tcggcacaaa ttttaatcga
     6661 ttattattga taatttataa gatgaaactt ttatcacaaa acaaaatata ttttctgttt
     6721 cacatgtcca ataattaaaa caaaccctgt atgaaaaatt tctttccgtt ttttttatgt
     6781 gttacattaa acattgtaac cttcgcgcag tcggatacct ggtcggtcaa attttccaat
     6841 gccattatca gccggtatca gcctaccatc aatgccatga ccaataaggg atgggaatac
     6901 tctaacggga gcattctgca tggtatggaa aaggtatatc tgaatactca cgatgcagac
     6961 tacctgaact atatcaaagc ttatgtcgac agctatgtgg atgccagcgg aaacgtcacg
     7021 ggtctcggga tgaccgtaga caaaatccaa ccgggggtgt tatgtctttt cctgtatgaa
     7081 cagaccggcc aaaccaaata caagaccgct gccacccaga ttaaaaatta cgtcaacaat
     7141 ttttacaaaa cccctgacgg cggaatctgg cataaaagtg aacaaaatgt aacttcttac
     7201 aagtattaca acgtcatgat ggttgacgga atgtacatgc tgcacccgtt cctcaccaaa
     7261 tatgccagta tgttcaacga accggcactg tttgatgcag cgacttttca gctcctcttc
     7321 ctggcaagca aagttatgcc ccagcctatg acgcttccca aacatgcctg ggaatattac
     7381 gactacaaag catggtcaga tccttccacc catatgagca ccgacgtatg gtcgagaggc
     7441 accggctggt atatgatggc cctagctgat gtactcgagt acctccccac ctctcatgcc
     7501 aactatggcg caattctgga actgtttcag cgcatgtcgt cgggagtggc tgccaaccag
     7561 catcccacca ccggattgtg gtaccaggtg gttgacaaaa ggacagaaac aggcaactgg
     7621 acagaaagtt caggctcagg gatgttcgtg tatgccctga aaaagggagt ggagaaaggg
     7681 tggttgaatg ctgccaccta caatccggtg atcaacctgg gatggcaggg tctccaaacc
     7741 caaattgcaa tacttggcga cagcggacca cagatcaaac agttttgtgt ggctacggga
     7801 gtggtggaca atactgccgc ctattatgca ctgggcaaaa ccgattgtcc caccgcgtat
     7861 ccctggagcg gaacccagca ccctcacggt tactgtggaa ttctgatggc tgcctctgtc
     7921 atggaattca atacgataaa tgtcccggca accggagtag cggtgagccc cgcttccctt
     7981 acgctttctg tcggaaatac gcagcagctt acggccacgg ttgccccttc aaatgctacc
     8041 aacaagagtg ttacctggag cagcagcaac acatcggtgg ctacggtcag tcctaccggt
     8101 ctggttacgg ctgtggtccc aggaaacgct gtcatcacca ccacaacaca agacggcaat
     8161 ttcacatcct cttcatctgt taccgtgaat gccgtcattc cctgcaccac aatccaatac
     8221 caggctgaaa gcggaacgtt tacttccgga acaaccatcg acacgaactg ggccggatac
     8281 accgggaccg gatttatcaa cacacccaat gcaaccggaa gctacctgca gataaccgtt
     8341 acagccctga tcgcaggaac gcatgatttt accatcagat ttgctaacgg aacgaccacc
     8401 gaccgcaccg cctccgtcgc tatgaacgga tccactgtgt tacccagtct cagtatggct
     8461 gcaacgggat catggtccac ttggtccact tcagcttttt ctgttcccct tgttgccggg
     8521 acaaacacga tccgtttcac tgcactgact accggaggtt tggccaatat tgaccggatc
     8581 gatatgtgcc agaagtacat gccggtaacc ggtgtgaccg ttactcctgc atctgccacg
     8641 cttgcacccg gaggcacaca gcaacttacc gttaatgttg agcctactga tgcaatcaac
     8701 aaggttgttt cctggagcag cagcaacaca tcggtggcta cggtcagttc taccggtctg
     8761 gttacggctg tggctgcagg aaacgctgcc atcaccgcca ccacccaaga cggcagcttt
     8821 acagcaacct gtcagataac cgtttcggca gtctctgtta caggaataac cgtcagtccg
     8881 tcaagtgcca cgctggaaat cggcgaaaca acgacactga tcgcagcagt gtttccggtc
     8941 aatgcaacca acaaggttgt ttcctggagc agcagcaaca catcggtggc tacggtcagt
     9001 tctaccggtc tggttacggc tgtggctgca ggaaacgctg ccatcaccgc caccacccaa
     9061 gacggcagct ttacagcaac ctgtcagata accgtttcgg cagtctctgt tacaggaata
     9121 accgtcagtc cgtcaagtgc aacgctggaa atcggcgaaa caacgacact gatcgcagca
     9181 gtgctaccgg ccaatgcaac caacaaggtt gtttcctgga gcagtagcaa cacatcggtg
     9241 gctacagtca gttctgctgg gctggttacg gctgtggctt caggaaacgc tgtcatcatt
     9301 gtcaccacac aagacggcaa tttcacatcc tcttcatctg ttaccgtgaa tgccgtcatt
     9361 c
//
LOCUS       DAOPMW010000095         9326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8074_length_9326_cov_9.794413, whole genome shotgun sequence.
ACCESSION   DAOPMW010000095 DAOPMW010000000
VERSION     DAOPMW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8074_length_9326_cov_9.794413"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLU11_06435"
     CDS             complement(<1..245)
                     /locus_tag="PLU11_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HPN58789.1"
                     /translation="MRWLTVVSLLLLLSACDMREKSKEPKINKTMTDRFSPPDWVRGT
                     TIYEVNIRQYTPEGTFNAFAQHLPRLKELGVETLWFMP"
     gene            332..1543
                     /locus_tag="PLU11_06440"
     CDS             332..1543
                     /locus_tag="PLU11_06440"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPN58790.1"
                     /translation="MKQFDVPDFYRSPLIAAIKKKRKEADKMKKDFSPTLLDFGPVQI
                     SLARHFGFCYGVENAIDIAFRTVAENPGKRIYLLSEMIHNPQVNADLVSHGIQFLQDT
                     HGKQLIAFEQVTADDIVLIPAFGTTLAIEKQLQEKGIPTEKYNTTCPFVEKVWNRSEV
                     IAQKEYTIVIHGKPGHEETRATFSHAAANGPAVVVKDMEEAELLASFITGEKKADEFY
                     SVFNGQYSTGFDVTLHLKRLGVVNQTTMLASDTQAIADYLKKIIVAHTGDPAGFADTR
                     DTLCYATNDNQTAVSTMLESKADLAIVVGGYNSSNTSHLAELCEIQLPTFYISSAEKL
                     ISREEVLHFDFHQKLEKNSKGYLPDNVPVHILITSGASCPDAMVESVINKLLSFYEGA
                     ASMETVMSRFE"
     gene            complement(1596..3251)
                     /gene="glgP"
                     /locus_tag="PLU11_06445"
     CDS             complement(1596..3251)
                     /gene="glgP"
                     /locus_tag="PLU11_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPN58791.1"
                     /translation="MSFNKYSVPYPVEERYSKRVAYFSMEFATHQPLKIYSGGLGFLA
                     GSHLRSAYELRQNLIGVGILWKYGYYDQERNQDQTLDVSWNEKQYSFLEDTGIKFQIN
                     IHEHPVWVKALYLNPETFKTAPLFLLSTDLPENDYVSQTITHRLYDANVATKVAQFIL
                     LGVGGAKLIDLLGFNPELYHLNEAHGISAAFYLYKKYGHSIQEVKKRLVFTTHTPEEA
                     GNEKHDIHLCHKMSYFCGLTIDDVKKLYGNESDQFNHSLAALRFAHLANGVSQLHGHV
                     SRAMWSKYEAICPIISITNAQNWRYWADKQLYRFMEAGDDYGMDDRKKYLKKRAFEIV
                     ADQTGKIFHPDVFTIVWARRFAGYKRAGLITTAEEKFEEMIHNSKYPVQIIWAGKPYP
                     VDHPAISEFNQLVHLSKRYKNVAVLIGYELGLSKRLKQAADVWLNNPRVPREASGTSG
                     MTASMNGAVNFSTNDGWIPEFINHGHNGFVVPMADYDNMPVYEQDQYDLAKLYEILQN
                     EILPLYYENYSTWRQVMKNGMQDVRFQFDSNRMAHEYYDLLYK"
     gene            complement(3375..4370)
                     /locus_tag="PLU11_06450"
     CDS             complement(3375..4370)
                     /locus_tag="PLU11_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58792.1"
                     /translation="MSLIKLFLLLMAGHLLLTGCQSKKKPVLSGDEPVAVSDFIEFFP
                     PLTLGFQAGDTMLNKKEKDTLRISYKIFTQFVPDTVLTKLFGKGNKPGIWAIGKAGVP
                     DGETYLLIRAGTSAKKGWVLLAFDKKDKFITAIPALIPDNKKETSQSFILDKRYTITR
                     AVQRRNADGSQSEGKDVFVLNEATGQFMLILTEALDEKITELINPIDTLGRKHKYAAD
                     YGTGKMNLVSVRDGRKADRISFFIHFEKDNGACTGELKGEAKWTSPGKAVYQEDGDPC
                     VLNFSFNSNSVTLSETGCGARHGLNCNFNGSYARRKYTKPVKPAAKNTGSKTGKK"
     gene            4483..5145
                     /locus_tag="PLU11_06455"
     CDS             4483..5145
                     /locus_tag="PLU11_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPN58793.1"
                     /translation="MNYSVSEENYIKTIFHLQKKEGTVTTNELAGELKTRPASVTDMM
                     KKLKAKKLLHYQPYQGFRLTTDGSKVALGIIRRHRLWEYFLAEKLKFTWDEVHEVAEE
                     LEHVSNKKLIDKLDAYLGFPKKDPHGDPIPDAQGKIESGKKIRLPEVPLHTSCLVSGV
                     TNQSGEILELLKHKKIRIGTTLEVRKRFEFDQSLEIRTGKQTIINISRELAENILISY
                     ES"
     gene            5135..7018
                     /locus_tag="PLU11_06460"
     CDS             5135..7018
                     /locus_tag="PLU11_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPN58794.1"
                     /translation="MSRDKEPYQSLSEVHGTVDTTQVNGKGWRKLLAFFGPAYLVSVG
                     YMDPGNWATDLAGGSQFHYKLIWVLLMSNLMALLLQSLSARLGIVRGRDLAQANRETY
                     PRYINYALYGLAEIAIAATDLAEILGMAIGIQLLTGLPLIWGVLITVLDTFLFLLLQR
                     MGMRKMEAFIIALIAIIGLSFLTEIILAKPDLREVAKGFVPRIPNDTALYIAIGIIGA
                     TVMPHNLYLHSALVQTRKIRRDEKGIKRALRMNFIDSAIALNLAFFVNAAILVLAGKV
                     FFESGRTDVGEIRTAHELLKPMLGSELAPVLFAVALIAAGQSSTITGTLAGQIVMEGY
                     LHLRIAPWMRRLLTRLIAVIPAVIVILINGEDNIDSLLILSQVILSLQLGFAIIPLIH
                     FTSDKKKMGTFAIKPFTIVLASLITAVIVYLNIRMVIEQAGGFFSDSGSLLWKAVIII
                     AALLYLLLLVVAVIYPLLSKKQADPLRIHPQATPLSLQQIPEYNQIAVALDFSENDQK
                     LMLHAIGQGNKKTRYILIHVVESAATRIIGKEADDLETRKDQEHLDEYVRQLKEKGIP
                     AIGLLGFNDRAKEIARLVKENQGDMLVIGAHGHSGLKDLIYGQTVDAVRHELDIPVLV
                     VSR"
     gene            complement(7029..7628)
                     /locus_tag="PLU11_06465"
     CDS             complement(7029..7628)
                     /locus_tag="PLU11_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPN58795.1"
                     /translation="MDTVRAFMIYLQKLSDITEEEFNKHLLPVCKVRRFAKKEVITRA
                     GEMENHFNFIVKGMARKYYKKGHQEINVQISMEGHMMLCQESFHSRQPSEYTIEAIEP
                     TTLVSISYEDAETMFASSQRMEHLGRIIVTYSMVIKDKWQMQLIKMTPRERFVSFVTK
                     NPELMQRVPQKYLASYLNIKPETFSRFKHLIRNHQVSVE"
     gene            7819..9051
                     /locus_tag="PLU11_06470"
     CDS             7819..9051
                     /locus_tag="PLU11_06470"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HPN58796.1"
                     /translation="MTQKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLEVDIKYYD
                     LGIEYRDQTDDQVTVDAANAIKEYGVGIKCATITPDEARVKEFSLKQMWKSPNGTIRN
                     ILDGTVFREPIVISNVPRLVTNWTAPIIVGRHAFGDQYRATDTVIKGKGKLTMTFTPE
                     GGGEPQTFEVFNFKGDGVAMSMYNTDESIMGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFQEIFENEFKAQYEAAGIVYEHRLIDDMVASALKWNGNFVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLITPDGKTMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIF
                     AWTRGLAFRGKLDGNQPLIDFCLALETVCIETVEEGKMTKDLAVCIHGNKVKHGDHFL
                     YTEEFLEAIDSNLQKKLK"
BASE COUNT         2583 a         2073 c         2148 g         2522 t
ORIGIN      
        1 ggcataaacc agagtgtttc cacacccagt tcttttaaac ggggcaagtg ttgtgcaaaa
       61 gcattgaatg tgccttccgg ggtatattgg cggatattta cttcataaat cgtggtgccc
      121 cgcacccaat ccggtggact aaaccgatct gtcattgttt tattaatttt gggttccttg
      181 cttttttccc gcatatcgca ggcggataaa agaagcagca gtgaaacaac cgttaaccag
      241 cgcatggtca tgttttgggc aagataagaa gaacatttga ggaagtttac tgttgtagga
      301 gcatccgttt tccaatcatt aactttgttc catgaagcaa ttcgatgtac cggattttta
      361 ccgcagtccc ctgatcgctg caattaaaaa aaagcggaaa gaggctgata aaatgaaaaa
      421 ggatttcagc cccaccctgc ttgatttcgg cccggtacag atttccctgg cccgccattt
      481 cgggttctgt tatggggtcg aaaatgccat tgatattgct ttccggactg ttgctgaaaa
      541 tcccggcaaa cggatttacc tgctcagtga aatgatccat aatccgcagg tcaatgccga
      601 tctggtgagc catgggattc aatttctgca ggatacacat ggcaaacagc tgatcgcctt
      661 tgaacaggtt acagccgatg atattgtgct catcccggca tttggcacca cgctcgctat
      721 tgaaaaacaa ctacaggaga aaggtattcc tacggagaaa tataatacca cctgtccctt
      781 tgtggaaaag gtttggaacc gcagcgaggt tattgcgcaa aaagagtata cgattgtcat
      841 acatggcaag cccggtcacg aggaaacaag ggctacattt tcacatgccg cagcgaatgg
      901 tccggcagtg gtggtaaaag atatggagga agccgaatta ctggccagct ttatcaccgg
      961 agaaaagaaa gcggatgaat tctattccgt attcaatggg caatattcca cgggatttga
     1021 cgtcacccta cacctgaaac gtttaggcgt ggtgaatcaa acgaccatgc tggcgagcga
     1081 tacacaggct attgccgatt atctcaaaaa aataatcgtg gcgcacacag gggatccggc
     1141 cggttttgcc gatacccgtg ataccctgtg ctatgccact aacgataacc agacggcagt
     1201 cagcaccatg ctggaatcga aagctgacct ggccatcgtt gttggcgggt ataactcatc
     1261 caacacttct cacctggcag agctatgcga aatacaattg cccacctttt atatcagcag
     1321 tgccgaaaag ctcatttcac gggaagaagt ccttcatttt gattttcacc agaaattgga
     1381 aaaaaacagc aaagggtatc tgccggataa tgttccggta catattctta tcaccagcgg
     1441 cgcatcctgt ccagatgcca tggtggaatc ggtaatcaat aaattactca gcttctatga
     1501 gggtgctgcc tcaatggaaa cggtgatgag ccgattcgaa taaaaaaacc cctgaagacc
     1561 aggggttctg cgtaaatcgg tatattttat tcctgttact tgtacaggag atcataatat
     1621 tcatgtgcca tccggttact gtcaaactgg aagcgtacat cctgcattcc gttcttcatc
     1681 acctgtctcc aggtactgta gttttcataa taaagtggca gtatttcatt ttgcagaatt
     1741 tcgtagagtt tagccaggtc atactgatcc tgttcgtaga caggcatatt atcataatcc
     1801 gccatcggaa ccacaaaccc gttatggcca tgattgataa actccgggat ccatccgtca
     1861 ttggtggaga agttcactgc gccgttcatg gaagccgtca taccgctggt accggatgct
     1921 tcgcgtggta cacgtggatt atttaaccac acatccgcgg cctgtttcag ccgcttggac
     1981 aatccgagtt cgtatccgat cagtacggct acatttttat agcgtttgct caggtgaacc
     2041 agttgattaa attcactgat agccggatga tccaccgggt aaggtttacc ggcccaaatg
     2101 atctgcaccg ggtactttga attatggatc atctcttcaa atttttcttc agcggtggtg
     2161 atcagtcccg ctcttttgta tccggcaaaa cgtctggccc aaacaatggt gaatacatcc
     2221 ggatggaaaa ttttaccagt ctgatcggcc actatctcaa acgccctttt cttcaggtat
     2281 tttttccggt catccattcc gtaatcatcg cctgcttcca taaagcggta caattgtttg
     2341 tcggcccagt acctccagtt ctgcgcattg gtaatggaaa tgatagggca aatagcctca
     2401 tacttgctcc acatggctct ggatacatgg ccatgcagct gggatacgcc attggccagg
     2461 tgcgcaaagc gcagggccgc cagggaatga ttaaactgat cgctttcatt cccgtataat
     2521 ttctttacgt catcaatggt gagtccgcag aagtaactca ttttatgaca aagatgaata
     2581 tcgtgctttt cgttgcccgc ttcctccggt gtgtgggtgg taaacaccag gcgttttttt
     2641 acttcctgta tactgtgccc gtacttttta taaagataga aggcagcaga gatcccatgc
     2701 gcttcgttca ggtgatacaa ttcaggatta aatcccaaca ggtctatcaa tttagcacct
     2761 ccaactccaa gcagaatgaa ctgggctact ttagtagcca cattcgcatc atataaccgg
     2821 tgcgtgatgg tttgggaaac atagtcgttt tccggtaaat cggtggaaag cagaaagagc
     2881 ggagccgttt taaaggtttc cggattcagg taaagggctt tcacccacac cggatgttca
     2941 tgtatgttga tctggaattt gatcccggtg tcttccagaa aactgtattg cttttcattc
     3001 caggaaacat ccagggtctg atcctggttg cgttcctgat cgtaataacc atatttccag
     3061 agaatcccca ccccgattaa gttctgacgg agttcatatg cacttcttaa atgagaaccg
     3121 gccagaaagc ccagacctcc gctataaatt ttaaggggtt gatgtgtggc aaattccatg
     3181 gagaaatagg ccactctttt actgtaacgt tcttcgaccg ggtaagggac tgaatattta
     3241 ttaaaactca taatgtgttc tttttacacg aaggcgcaag ttagtgctaa attatgatat
     3301 aaactaacag gctcattctc aggttaattc attaaaaaaa atggggcaat cgcgtaggaa
     3361 atacgggcaa aattctactt tttacctgtt ttactcccgg tatttttggc agccggtttc
     3421 actggtttgg tatattttct ccgggcatag gacccgttaa agttacaatt taatccatgt
     3481 ctcgcaccac aaccggtttc ggaaagtgtt acgctattgg aattgaatga aaaattaagc
     3541 acacaggggt ctccatcttc ctggtaaacg gctttgccgg gactggtcca ttttgcttca
     3601 cctttaagtt cacctgtgca ggctccatta tctttttcaa aatgaataaa aaaactaatg
     3661 cggtcggcct ttcttccgtc acggacagaa acgaggttca tttttccggt accataatcc
     3721 gccgcatact tatgtttccg accgagtgta tcgataggat ttatcagttc cgtgattttc
     3781 tcgtccagtg cttctgtcag aatgagcata aactgcccgg tggcttcatt gagtacaaag
     3841 acatcttttc cttcactttg ggaaccatcc gcattccttc gttgaaccgc cctggtgatt
     3901 gtatagcgtt tatccagtat aaacgactgg ctggtttctt ttttattatc tggaatcagt
     3961 gcgggaattg cggttataaa tttatccttt ttatcaaatg ccagtaagac ccatcccttt
     4021 tttgccgatg taccggccct gatcaataaa taggtttctc catccggaac cccggctttc
     4081 ccgattgccc agataccggg tttattgcct tttccgaata acttagtcag taccgtatcc
     4141 ggtacaaact gcgtaaatat tttataactg atccgtaagg tgtctttttc ctttttattc
     4201 agcatcgtat cccctgcctg aaaaccaagg gtaagagggg gaaagaattc aataaagtct
     4261 gaaacggcga ccggttcatc accggataaa accggttttt tctttgactg gcaaccggtc
     4321 aagagcaaat ggcctgccat cagcagaagg aataatttta tcagggacat aagatcagat
     4381 ataacagccg gaaagatagt ggtttctccg gtcaatgtca accggtaatt tggccgttac
     4441 ctaaacttaa tttagatttg tctaaattat tttttaggca atatgaacta ctcagtcagt
     4501 gaggaaaact atatcaaaac cattttccat ctgcagaaga aggaagggac agtcaccacg
     4561 aatgaactgg ccggagaact gaaaacccgt ccggcatcgg ttacggatat gatgaaaaaa
     4621 ctgaaagcta aaaaactgct tcattatcag ccttaccagg gtttccggct gaccacagat
     4681 ggcagtaaag tggcgctcgg tattatacgc cgtcaccggt tatgggagta tttcctggcg
     4741 gagaaattga aattcacctg ggatgaagtg catgaagtag cagaagaact ggagcatgtg
     4801 agtaataaaa aactcattga caaactggat gcttacctgg gatttcccaa aaaagatccc
     4861 cacggggatc cgattccgga cgcccaaggt aaaattgaaa gtggaaaaaa aatcaggtta
     4921 cccgaagtcc cccttcatac aagctgcctg gtgtctggtg tgactaatca atccggagaa
     4981 attctcgaat tactgaaaca taaaaaaatc aggatcggca cgaccctgga agtgcggaaa
     5041 aggtttgagt ttgatcagtc acttgaaatc aggacgggca aacaaacaat aataaatatc
     5101 agccgagagc tggcagaaaa tatattgatc tcctatgagt cgtgataagg aaccatatca
     5161 atcactgagt gaggtacatg gtacggtaga taccacacag gtcaacggta aaggctggag
     5221 aaaattgctt gccttctttg ggccggctta cctggtaagt gtcggttata tggatccggg
     5281 caactgggct actgatctgg ccgggggcag tcagtttcat tataaactga tctgggtgct
     5341 gctcatgagt aacctgatgg ccctcttgct gcagagttta tccgccagac tgggaattgt
     5401 caggggacgt gacctggcac aggccaacag ggaaacctat cccaggtata tcaactatgc
     5461 attatacggc ctggcggaaa ttgcaattgc ggctaccgac ctggcggaaa ttctgggtat
     5521 ggctattggt attcagttac tcaccgggct gcccctcatc tggggtgtat tgataacggt
     5581 actggacacc tttcttttcc tgttattaca gcggatgggt atgcggaaaa tggaggcttt
     5641 tatcattgcc ctgattgcta tcattggcct ttcgttccta acagaaatca tactcgccaa
     5701 accggatctg cgggaagtgg ctaaaggatt tgttccccgg atccccaacg atacggccct
     5761 ttatattgcc attggtatca ttggtgccac agtaatgccg cataacctgt acctgcactc
     5821 cgcactggta cagacacgta aaatcaggcg ggatgaaaaa ggtattaagc gagccctccg
     5881 gatgaatttt attgatagtg ccatcgcact gaatctcgcg ttttttgtca atgcggccat
     5941 tcttgtcctg gcgggaaaag ttttctttga atcaggccgg accgatgtcg gagaaatcag
     6001 gacagcgcat gaattgctga agccaatgct gggcagtgaa ctggctccgg tgttgtttgc
     6061 agtagccctg attgccgccg gacaaagttc caccattacc ggtaccctcg cggggcagat
     6121 tgtaatggaa ggttatttgc atttacggat tgctccctgg atgcgacggc tcctgacccg
     6181 acttattgcc gtaattccgg ctgttattgt aatattgatc aatggggaag ataatattga
     6241 cagtctcctg attttaagtc aggtgatcct gagtctgcaa ctcggatttg ccatcattcc
     6301 cctgatacat tttaccagtg ataaaaaaaa gatgggcaca ttcgccatca aaccctttac
     6361 cattgtactg gcttccctga tcaccgctgt aattgtatac ctgaatatcc gcatggtgat
     6421 agaacaggcc ggtggttttt tcagtgattc aggcagcctg ttatggaagg ccgtgattat
     6481 tattgcggcg ctgttatatc ttttattact ggtggtggca gtcatatacc cgttactgag
     6541 taaaaaacaa gcggacccgc tccgcattca cccacaggcc actccccttt ctctgcagca
     6601 gatacccgag tataaccaga ttgcggtggc cctggatttc agcgaaaatg atcaaaaact
     6661 gatgttgcat gccatagggc agggaaacaa aaaaacccgc tacatcctga tccatgtggt
     6721 ggaaagtgcg gctacccgga tcataggtaa agaagccgat gacctggaaa cccggaaaga
     6781 ccaggaacac ctggatgagt atgtacggca attgaaagaa aaaggaatac cggctatcgg
     6841 gttgcttggt ttcaatgaca gggcaaaaga aatagcccgt ttagtgaaag aaaaccaggg
     6901 agatatgctg gtcatcgggg cgcatggtca tagcgggttg aaagacctta tttatggtca
     6961 gacggtggat gctgtaagac atgaattgga tattcccgta ttagtggtca gtcgttgaaa
     7021 gcaggcagtc attccaccga cacctggtga ttccggatca ggtgtttaaa gcggctgaac
     7081 gtttccggtt tgatattgag ataagaagca aggtatttct gagggacccg ttgcatcaat
     7141 tcaggattct tggtaacaaa actgacaaac cgttccctgg gcgtcatttt gataagctgc
     7201 atctgccatt tatctttaat caccatggaa tacgtgacga tgatccggcc tagatgttcc
     7261 atccgctggg atgaagcaaa catcgtttcc gcatcctcat aggaaataga aacaagcgtg
     7321 gttggttcaa tcgcttcaat ggtatattct gacggctgac ggctgtgaaa ggactcctgg
     7381 caaagcatca tatgcccttc catcgaaatc tgtacattga tttcctgatg ccctttttta
     7441 taatatttac gggccatccc ttttacaata aaattgaaat ggttttccat ttcaccagcc
     7501 cgggtaatta cttccttctt ggcaaaacga cgcactttac aaacaggtaa caagtgtttg
     7561 ttaaactcct cttccgtgat atcggatagc ttctggaggt agatcataaa agcccgtact
     7621 gtatccatat ttgagctatg tgatataaaa ggtacgcttt ttaattgatt tgaatcaatt
     7681 cgacagaata caattcaacg gtaaaactaa ggggtcaaca atcatgcacc tatgacatta
     7741 gtcacatgtg ataaaatagt aagtttgtag ctgcctgata acgatcaggt tttcaatgta
     7801 aacccaaacc aaacttgtat gacacaaaag atcaaagttg cgaacccggt cgtagagctg
     7861 gatggcgatg aaatgacccg gattatctgg aaattcatta aagacaagct tattctgcct
     7921 tatctggaag tggatatcaa atattatgac ctgggaatcg aataccgtga tcagaccgat
     7981 gaccaggtga cggtggacgc cgccaatgcc attaaagaat atggggtggg tatcaagtgt
     8041 gccacgatta cgccggatga agcaagggtg aaggagttca gtctgaaaca aatgtggaag
     8101 agtccaaatg ggactatccg gaatatactg gatggcaccg tattccgtga accgattgtg
     8161 atcagcaatg taccccgcct ggtaaccaac tggaccgccc cgatcattgt gggccgtcat
     8221 gcatttggcg atcagtaccg ggcaaccgat accgttataa aaggcaaagg gaagctcacc
     8281 atgaccttta ctcctgaagg aggtggcgaa ccccaaacct ttgaagtatt caatttcaaa
     8341 ggcgatggcg tggctatgtc catgtataat actgacgaaa gtattatggg ttttgcccgc
     8401 agctgcttta atatggccct gagtaaaaaa tggcccctgt acctttccac caagaacacc
     8461 atcctgaaaa aatatgatgg ccgtttcaag gatattttcc aggaaatctt tgaaaacgaa
     8521 ttcaaggcac aatacgaagc cgccggtatt gtatacgaac accgcctgat cgatgatatg
     8581 gtcgccagtg ccctgaaatg gaatggtaat tttgtatggg cctgtaagaa ttatgatggt
     8641 gatgtacaaa gcgatacggt cgctcagggc ttcggttcac tcggactgat gacttctgta
     8701 ttgatcacgc cggatggtaa aaccatggaa gcggaagccg cccatggaac cgttacccgt
     8761 cactaccggg atcaccaggc cggaaaaccc acatctacca acccgatcgc ttctatcttt
     8821 gcctggaccc gtggactcgc gttccgtggt aaactggatg gcaatcaacc gttgatcgat
     8881 ttctgcctgg cactggaaac cgtttgtatt gaaacggtgg aagaaggcaa aatgaccaaa
     8941 gacctggccg tttgtatcca tggaaataaa gtgaaacacg gcgatcattt cttgtatacg
     9001 gaagagttcc tggaggcgat tgacagtaat ttgcagaaga aactgaaata atttcaaatt
     9061 gattccaaag aggttgtcgg acaaacaacc tcttttttta tacccgttaa aaaatcaaaa
     9121 ttcaaaagta aaaagtaaaa aatctcatag cagatcgatt ctggactata aataccattc
     9181 tgaatccagg aaaatcaggt atggtgccat ccgttttgaa tagtacagac taaaggcttt
     9241 tgacttttta atttttactt ttgacttaca aatctggtta tgaaaaaaag aacagactat
     9301 atcacctggg acgaatactt catggg
//
LOCUS       DAOPMW010000096         9309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8102_length_9309_cov_9.290793, whole genome shotgun sequence.
ACCESSION   DAOPMW010000096 DAOPMW010000000
VERSION     DAOPMW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8102_length_9309_cov_9.290793"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLU11_06475"
     CDS             <1..366
                     /locus_tag="PLU11_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPN58797.1"
                     /translation="VKEALNITTEGAGEAVMHSIHQELNRQLQHDISFITLSEKGVFY
                     QQGNVGYIIPSHIRNIADVSGAGDTVIAVASLVYAVTRDVKLMAETANIAGGLVCEEV
                     GTAPINSEKLMQECELLLN"
     gene            complement(363..1031)
                     /locus_tag="PLU11_06480"
     CDS             complement(363..1031)
                     /locus_tag="PLU11_06480"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HPN58798.1"
                     /translation="MFTRKLTQSDIFIIAANLLPVYGVWFLGWPPVEAFTVYAMETMI
                     VGIMTVLKLLVCTIAKGKDDWSNNGTVTKMSGFFFIFFFIVHFGLFALVQTSIFAETA
                     KIGPPGSGMFHFFFNWYKYINTEIGYMLLAFIIGYLAKSFIPFLLNGDYKRQPMMLIM
                     FMPYGRIFVQQFTVILGSMFLSLGAGKMFILIFVLAKLAFELLMNFEALLKKTTEEMN
                     KEKN"
     gene            1097..2089
                     /locus_tag="PLU11_06485"
     CDS             1097..2089
                     /locus_tag="PLU11_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPN58799.1"
                     /translation="MSSYAIVIHGGAGTILPAAMTTELEQSYKAALQEAMDAGFRCLT
                     AGGTSIEAVKAAVMSLEDCILFNAGRGAVFAKDGSQEMDASIMDGSNRMAGAVAAVRN
                     IRNPVELAYVVMTKSQHVMLNGKGAQEFAKIHGLKTEPDEYFYSEFRHQQWKQMQDTD
                     AMALDHSVTIPATASSDDFAEGKPSFTKVSEDKKFGTVGAVALDQYGNIAAATSTGGM
                     TNKQWGRVGDSPIIGAGTYANNMTCAISCTGHGEPFIRAVTAYDVSCLMEYKGLSLQE
                     AMHIVVHDKLLKIDGEGGMIGVDAAGNTAMVFNSEGMYRGRRDSEGLNETLIYK"
     gene            2120..2794
                     /locus_tag="PLU11_06490"
     CDS             2120..2794
                     /locus_tag="PLU11_06490"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPN58800.1"
                     /translation="MKKYAIIVAGGSGIRMGGNLPKQFMLLRDKPVLYYTLKAFIESY
                     EDLQVILVLPMDYTDMGQEIIDAFFDKDRIRITAGGDTRFQSVKNGLALVEEESVVFV
                     HDGVRCLLTKELIHRCYKQAAETGTAIPAIPSKDSIRLITDEGNAAFDRNRVMLIQTP
                     QTFHSKIIVPAFQIDYKDKFTDEATVAEAYGIKVSLVEGEETNIKITRPVDLLLAEQI
                     LAEREA"
     gene            complement(2796..3704)
                     /locus_tag="PLU11_06495"
     CDS             complement(2796..3704)
                     /locus_tag="PLU11_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN58801.1"
                     /translation="MTDGNTIQYLTRLQIDTAKWDQCISRASNGRIYAFAVYLDTMSA
                     NWDALVLNDYEAVMPLTWNSKYGIQYLYQPFLAAQLGVFGSEEVEAFIEAIPGHFRYI
                     DIYLNSANTNAVKGDDVQLRDNYVLALHHPYEKLVTDYRENIRRNIKKAKQAGCIVEK
                     GFEVETVITLATAQMQLHDKNVEENTGRFRQLYQLLKAVNKATTYGISLHGEMIASCV
                     FFFSNNRAYYILVGNHPNGKTIGASHALIDAFIADHAGQNLLLDFEGSDIRNLAFFYS
                     SFGATQEIYPALKINRLPFYLRWLKK"
     gene            complement(3704..6613)
                     /locus_tag="PLU11_06500"
     CDS             complement(3704..6613)
                     /locus_tag="PLU11_06500"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN58802.1"
                     /translation="MKKILLLLILSGLLTRSFCQLQTPSVKTSEVKAGDWRLSYWQQE
                     VNYTIDVSLNDVDHSLTGFEQLEYINNSPDTLRFIWFHLWPNSYKNDKTAFSDHMLEN
                     GSTKFYFSNKEERGYINKLDFKVDGVTAQLEDHPQHIDIAKLVLPAPLAPGQKIAIST
                     PFHVKLPYNFSRGGHDGQSYQATQWYPKPAVYDKEGWHPMPYLDQGEFYSEFGSFTVK
                     ITVPKNYVVAATGELQNQDEKDWLKTRSSYTWEPIKQRIKTAGGQFKTTYQLFPDSEK
                     EIKILTYKQDRVHDFAWFADKRFIVNTDTCRLATGKIIDVYSYYTPAEKETWQHSVAF
                     AKAATRHYSDWVGEYPYTVVSVVQGPESFGGGMEYPTITVLSPSDTPRDLDYTIAHEI
                     GHNWFYGILASNERNYPWMDEGINSYYEYRYQQKKNDHFMLINEQRMLETQVAELKDQ
                     PINTPAPEFTENNYALIAYYKTAKWMELLSKELGEDVFNKAIQAYFNQWKFRHPAPAD
                     FKIALEQSSGRQLDSVFALLDKKGNLPNVIKKGIAVQDPFSAFGNALLGGITDKKSVT
                     TFYPSFGLNNYDKLMVGVMVTNLRFPPNKFQFLLVPLYGLNSKALNGIGFVNYSSYPK
                     SVFRKVDIGISAASFSGDRFVDKSQSFISDNPDPFLRFTKWVPGIRLTLAEKKARSTF
                     NRYFQFKTFLINEDQLQFFRDTVIVGIDTTILSKYRTNPESRVLNQFKFVVENNRVLY
                     PYSGELKVEQGKDFLRTAFTGKYFFNYAKEGGLNLRVFAGKFFYTGAKTFSKQFATDR
                     YHLNMTGANGYEDYTYSDYFFGRNEFDGTASQQIMERDGAFKVRTDLLAEKVGRTDNW
                     LFAANFSTTIPNSINPLSILPVKIPLKLFFDIGTQSGAWEAGATTDRFLFDAGLQIPL
                     LKEIVNIYIPLLYSNVYKDYFQSTIAKKDRFWKKISFSIDISNFSFRKIDRNLDF"
     gene            complement(6917..8332)
                     /locus_tag="PLU11_06505"
     CDS             complement(6917..8332)
                     /locus_tag="PLU11_06505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPN58803.1"
                     /translation="MQSGKQISAGWYVAADYLTAALAWFCYYHWRSWMLNDAGAYPIS
                     QTSWTLIFLIVPAGWLMLYTLAGTYNHLYKKSRLAEFTLTFVCSLIGAIALFITFVFN
                     DYDPKNGYTYFYYAFFVLLGIHFFLTFIGRWLILRKVKRQLLSGNVFFNTLMIGSQES
                     AIRIFRETELNLRDGGYRYTGYVTHDHNGKNGISKLLPRLGEIEEIERIIDKEDIKLV
                     VLALEKTEQPLLENLINRLSEKDVEVKIQPSTLDILSGSVKTMSVMGAALIDLQTGLM
                     QQWEQNIKRLIDVLASLISLILLSPFLLYLALRVKLSSAGPIFYSQERIGFKGRPFRM
                     YKFRSMVKDAEKDGPALSSENDSRITRWGKVMRKWRFDELPQLWNILIGEMSLVGPRP
                     ERRFYIDQIIARYPFYKYLLKVKPGLTSWGMVQYGYAENVDQMIERSKFDLLYIENIS
                     LILDFKIMIHTVRIIFKGKGK"
     gene            complement(8332..>9309)
                     /locus_tag="PLU11_06510"
     CDS             complement(8332..>9309)
                     /locus_tag="PLU11_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN58804.1"
                     /translation="VMLKIGVIGVGHLGKFHLNNWKEIAGVDIVGFYDPHDENAKEVA
                     EKYQLPRFLDAEALIESCDAVDIVAPTNFHFEWCEMAIKKGKHVFVEKPLANTMEEAR
                     QLVKLAAESGIKFQVGHVERFNPAFLALKDMDLKPMFIEVHRLAQFNPRGTEVSVILD
                     LMIHDIDIILSIVKSDVKSISASGVAVMTETPDIANVRIEFHNGCVANLTSSRISMKK
                     MRKVRLFQKDSYIGIDFLNKKTEIIKLKEETDLNVFAFDIETPSGKKTIAMANPAVPE
                     VNAIKLELEEFRDAIKNNSRTVVNEMDGLMAMDVAHQILEKIGHNVIAH"
BASE COUNT         2440 a         2037 c         2283 g         2549 t
ORIGIN      
        1 gttaaagaag cgttgaatat cacaacagag ggcgccggag aagcggtcat gcattccatt
       61 caccaggaac tgaaccgtca gttacaacat gatatttcat ttatcacttt gtcggagaaa
      121 ggggtttttt accagcaggg aaatgtgggg tatattattc catcccacat ccggaacatt
      181 gcggatgtgt ccggtgccgg tgacacggtg atcgcagtgg cttcgctggt atacgcggtt
      241 acccgggatg taaagctgat ggcggaaact gccaatattg ccggcggttt ggtatgtgaa
      301 gaagtgggaa ctgcaccgat caatagtgaa aaactaatgc aggagtgtga attgctgctg
      361 aattaatttt tctctttatt catttcttcc gttgtctttt tcagtaaagc ctcaaagttc
      421 atcagcaatt caaaggcgag ttttgccaat acaaatatca ggataaacat ttttccggct
      481 ccaaggctga ggaacatact gccaaggatc acggtaaact gttgtacaaa tattcttccg
      541 taaggcataa acataatcag catcattggt tgtcttttat aatccccgtt gagcagaaag
      601 gggatgaaac tctttgctaa atagccgatg ataaaggcca gcaacatata gccgatctct
      661 gtattaatat acttgtacca attaaagaag aaatgaaaca tgccgctacc gggcggacct
      721 attttagccg tctcggcgaa aatagaagtc tggaccaggg caaaaaggcc aaaatgcacg
      781 ataaagaaaa agataaagaa aaagccactc atttttgtca cggtcccgtt attgctccag
      841 tcatcctttc ctttggcgat ggtacatacc agtagtttta acaccgtcat tatacccaca
      901 atcatggttt ccatggcata gaccgtaaag gcttccaccg gcggccagcc caggaaccat
      961 acaccatata ccggcaataa gttggcggca ataataaaaa tatccgattg agtcagtttt
     1021 ctcgtgaaca tgttgtcagg tgtttcggat acaaaatacc taatttcgtc agtaattaaa
     1081 atacccagta acacgtatgt ccagttatgc aattgtcatt catggaggag caggtaccat
     1141 tcttccagca gctatgacca ctgaattgga gcagtcttat aaagcggcgc tgcaggaagc
     1201 aatggatgcc gggttccgct gcctgactgc agggggtact tccattgaag ctgtgaaagc
     1261 ggcggtgatg tcactggagg attgtatttt attcaatgca ggccggggag cggtatttgc
     1321 caaagacggc agccaggaaa tggatgcctc cataatggac ggatcaaacc ggatggccgg
     1381 tgcggttgcg gctgtacgga atatccggaa cccggtggaa ctggcttatg ttgtgatgac
     1441 taaaagccag cacgtgatgc tcaatgggaa aggggcacag gagtttgcca aaattcatgg
     1501 tttgaaaacc gagcctgatg aatattttta ttccgaattc cgccatcagc aatggaaaca
     1561 aatgcaggat acggatgcaa tggctttgga tcattctgta acaataccgg caacggcttc
     1621 ctctgacgat ttcgcagaag gtaagccctc cttcactaaa gtttcggagg acaagaaatt
     1681 cggaaccgtt ggtgcagtgg cgctggatca gtatggcaat attgcggctg ccaccagtac
     1741 aggcggtatg accaataaac aatggggacg ggtgggggat agtccgatca tcggtgcagg
     1801 tacgtatgcc aataatatga cctgtgccat cagttgcacc ggacacggag aaccgtttat
     1861 ccgggccgtc accgcctatg atgtgagttg cctgatggaa tacaaaggcc tttcgctgca
     1921 ggaagccatg catatcgtgg tacatgataa attactgaag atcgacgggg aaggaggcat
     1981 gatcggggtg gacgcggccg gcaatacggc catggtgttt aacagtgaag gcatgtaccg
     2041 cggacgcaga gacagtgaag gattgaatga aacattaatc tataaataat aattcaccgg
     2101 tttccctaca tttgcgccca tgaaaaaata cgcaatcatt gttgccggcg gttcaggtat
     2161 ccggatggga ggaaatcttc ccaagcagtt tatgctgctc cgggataagc cggtgcttta
     2221 ttatacactc aaagcattta tagagtccta cgaggacctg caggtaatcc tggtattgcc
     2281 catggattat accgatatgg gacaggaaat cattgatgca ttttttgata aggaccgtat
     2341 ccggattacg gccgggggtg atacccgttt tcagtccgtg aagaacggat tggccctggt
     2401 ggaagaagag tccgtggtat ttgtacatga tggagtccgt tgtctgctga ccaaagaact
     2461 tattcatcgt tgttacaaac aggccgcgga aacgggaacg gcgatcccgg ccattccatc
     2521 taaagacagt atacgcctga ttactgatga agggaatgcc gcttttgacc gcaaccgggt
     2581 gatgctgatc cagacacccc agacttttca tagtaagatc attgtgccgg ctttccagat
     2641 cgattacaaa gataaattca cggatgaagc cactgtagcc gaagcatatg gtattaaggt
     2701 ttccctggtg gaaggcgagg aaacgaatat caagatcacc cgcccggtag atctgttgct
     2761 ggcagagcag atactggcag aaagagaagc ctgatttatt tttttaacca cctgagataa
     2821 aagggaagcc ggttgatctt caatgccgga tagatttcct gtgtggcacc gaagctgctg
     2881 taaaagaagg ccaggttacg gatatccgat ccttcaaaat caaggagcag gttttgcccg
     2941 gcatgatccg caataaacgc atcgatcaaa gcatgcgagg ccccgatcgt ttttccgttt
     3001 ggatgattac cgacaagaat ataataagcc ctgttattcg aaaagaagaa tacacaggaa
     3061 gcgatcattt caccatgcag ggaaatgcca taagtagtgg ctttattcac cgctttcagt
     3121 aattggtaga gctgccggaa acgaccggta ttttcttcca catttttatc atgcaattgc
     3181 atttgtgcag tggcgagtgt aataacggtt tctacttcaa aacctttctc aacaatgcag
     3241 ccggcctgct ttgccttttt aatattgcgt cggatattct cgcggtaatc agtcactaat
     3301 ttttcatagg gatgatgcag ggcgagcaca taattatccc gtaactgaac atcgtcgcct
     3361 tttactgcgt ttgtattcgc gctgttgagg taaatgtcaa tgtaacgaaa atgaccgggt
     3421 atggcttcaa tgaatgcctc tacctcttca ctgccaaata cacccagctg tgcagccagg
     3481 aagggctggt atagatactg gattccgtat ttgctgttcc aggtgagtgg catcacggct
     3541 tcatagtcgt tcagcaccag ggcatcccaa ttcgcagaca tcgtatccag ataaacggca
     3601 aacgcataga tacggccatt ggaagcacgg ctgatgcact gatcccattt ggctgtatca
     3661 atttgtaacc ttgtcaggta ttgtatcgtg ttaccgtccg tcatcaaaaa tccaggttgc
     3721 ggtcgatttt acggaaactg aaattagaga tatcaatact gaaggagatt tttttccaga
     3781 accggtcttt tttagcaatg gtggactgaa aataatcttt atagacattg ctgtagagca
     3841 gcgggatata gatattcacg atttctttca gcagcggaat ctgtaagcct gcatcaaaga
     3901 ggaaacgatc cgtagtggca ccagcttccc aggcaccaga ctgtgtaccg atatcaaaga
     3961 ataacttcaa cggaatttta accggcagga tactcaacgg attaatggaa ttagggatcg
     4021 tggtactgaa attagccgca aacaaccagt tgtcggtacg tcccactttt tcagcgagca
     4081 gatccgttct taccttgaag gccccgtccc gctccatgat ctgctggctt gcggtgccat
     4141 caaattcatt ccggccgaag aaataatcac tgtaggtgta atcttcgtaa ccattagccc
     4201 cggtcatgtt gaggtgatac cggtccgtgg caaattgttt gctgaaggtt ttggcacccg
     4261 tataaaaaaa cttaccggca aatacccgga gattgagacc gccttctttg gcgtaattga
     4321 agaaatattt cccggtgaat gcggtacgga gaaaatcctt accctgttcc actttcaact
     4381 ctccgctata gggatacaat acccggttat tttccaccac gaatttaaac tgattgagga
     4441 cgcggctttc ggggttggtg cggtatttgg aaagtatggt ggtgtcaatg ccaactatta
     4501 ctgtgtcacg gaaaaactgc aactggtctt cattgatcag gaaagtttta aattgaaaat
     4561 agcggttgaa tgtggagcgg gctttctttt cggccagggt gagccggata ccgggtaccc
     4621 atttggtaaa gcgcagaaat ggatccgggt tgtcactgat aaaggactgt gacttgtcaa
     4681 caaaacgatc gccactgaag ctggccgcac tgatgccaat atccacttta cggaagaccg
     4741 atttgggata actactgtaa ttgacaaatc cgattccgtt cagtgcttta ctgttcagac
     4801 cgtaaagtgg taccagcaag aactggaatt tgttgggtgg gaagcgaaga ttggtgacca
     4861 tcacgccaac catcagttta tcatagttgt tgagcccaaa tgaaggataa aatgtggtaa
     4921 ctgatttttt atctgtaatc ccgcccaata acgcattgcc aaaggcggaa aagggatcct
     4981 ggacggcgat tccttttttt ataacattgg gaaggttccc ttttttatcc aggagagcga
     5041 aaacagaatc aagttgtctg ccactgctct gctcaagagc tattttaaaa tcagccggtg
     5101 caggatgacg gaatttccat tggttgaaat aggcctgtat tgctttgttg aaaacatcct
     5161 cccccagctc tttactgagt aattccatcc atttagctgt tttataataa gctatcaggg
     5221 cgtaattatt ttctgtgaat tcgggtgccg gtgtgtttat gggctgatct ttcagttctg
     5281 ctacctgcgt ttccagcatt cgttgttcat tgatcagcat gaaatgatca ttcttttttt
     5341 gctggtagcg gtattcgtaa taggaattaa ttccttcatc catccaggga tagttacgtt
     5401 cattactggc caggatacca tagaaccaat tgtggccaat ttcatgggca atggtataat
     5461 ccaggtcacg gggggtatct gagggcgaaa gaacagtaat cgtcggatat tccatgccgc
     5521 ctccaaagct ctcagggccc tgtaccacgc tgaccacggt gtaggggtat tctcccaccc
     5581 aatctgaata gtgacgggta gctgcttttg caaatgccac actgtgttgc caggtttctt
     5641 tttcagcagg agtgtaataa ctgtacacgt caatgatctt tccggttgcc aggcgacaag
     5701 tatctgtgtt gacgataaaa cgtttatccg caaaccaggc aaagtcatgg acccggtctt
     5761 gtttataagt caggatcttg atctcttttt ctgaatccgg aaaaagctgg taggttgtct
     5821 tgaattgacc gccggctgtt ttgattcgtt gtttaatggg ttcccaggta taactacttc
     5881 ttgttttcag ccaatctttt tcatcctggt tttgcaattc accggtggct gccaccacat
     5941 agtttttggg caccgtgatc tttacagtaa aacttccgaa ttcactgtaa aattctccct
     6001 ggtccaggta aggcatcggg tgccagcctt ctttgtcgta taccgccggc ttgggatacc
     6061 attgggtagc ctggtagctt tgtccatcat gtcctcccct ggaaaaatta tacggcagtt
     6121 ttacatggaa aggagtactg attgcaattt tttgtccggg tgcgagtggg gcgggtaata
     6181 ccagtttggc gatatcgata tgctggggat gatcttccag ttgtgccgtg accccatcga
     6241 ctttaaaatc cagtttgttg atatagcccc gttcttcttt attggagaaa tagaattttg
     6301 tgctgccgtt ctccagcata tgatcgctga aagcagtctt gtcgttttta taagaatttg
     6361 gccagaggtg aaaccagata aagcgcaggg tgtcggggga gttattgatg tattcgagtt
     6421 gttcaaaacc ggtgaggctg tgatcaacgt cgttgaggga aacgtcgata gtatagttga
     6481 cttcttgttg ccagtagctc agtcgccagt cgccagcctt gacctctgaa gttttaacgg
     6541 aaggggtctg cagttggcag aaagacctcg taaggagacc ggatagaata agcaggagaa
     6601 gtattttctt caatgggata gacagtttct caaaagtagt tctttcttgg gggtattgca
     6661 agaagtcaaa agtcaaaatt taaaagtaaa aagactgaga atcgtatatc tggaaaaatc
     6721 taatcaggcg ttttattgca gcaattaata tttcacccct aaaggggctc cgggcatttt
     6781 cttcgcactt gggctaatga aattacaccc ctaacggggt taagaaattg tcgttgacat
     6841 gaaagtctat gagttgtaga tatttcaacc agtcttcccc agaaggggct gtttactccc
     6901 tactgccgac tcccgactat ttcccctttc ctttgaagat aatcctcacc gtatggatca
     6961 tgatcttgaa atcgaggatc aatgagatgt tttcgatgta cagtaaatca aatttgctgc
     7021 gttcgatcat ctgatccaca ttttcggcat agccgtattg aaccatgccc caactggtca
     7081 gacccggttt tactttgagc aggtatttgt agaagggata gcgggcaatg atctggtcga
     7141 tatagaagcg gcgttcgggg cgggggccta caagactcat ttcaccgatc aggatattcc
     7201 agagctgggg taattcgtcg aagcgccatt tgcgcatgac tttgccccag cgggtgatgc
     7261 gggaatcatt ttcagaagac aaggccgggc catctttttc cgcatccttc accatcgagc
     7321 ggaatttata catccggaat ggtctgccct tgaagcctat tcgttcctgt gaataaaaaa
     7381 tgggacctgc cgaagaaagt tttacacgta gtgctaaata gagcaggaat ggggagagca
     7441 ggatcaggct gatcagggaa gccagtacat cgatcaggcg ttttatattt tgttcccatt
     7501 gctgcatgag tcctgtctgc aggtcgatca gtgcggcacc cattacactc attgtcttca
     7561 cggagccgga aagaatatcg agggtgcttg gctggatctt tacttccaca tctttttcac
     7621 tgagccggtt gatcaggttt tccagtaagg gctgttctgt cttttcgagg gcgagcacga
     7681 ccagttttat atcttcttta tcgatgatgc gttcgatctc ttctatttcc ccgagcctgg
     7741 gtaacaattt actaatgccg tttttcccgt tatggtcatg ggtaacataa ccggtatagc
     7801 ggtagcctcc gtcacggaga ttcagttcgg tttcgcggaa gatccggatc gcactctcct
     7861 ggctgccgat catcagtgta ttgaaaaaga cgttgccgct caggagctgg cgttttactt
     7921 tccgaaggat cagccagcgg ccgatgaagg tcaggaagaa atggataccc agcaaaacga
     7981 agaacgcata atagaaatag gtatacccgt ttttcggatc ataatcatta aaaacaaaag
     8041 tgataaagag tgcaatggca ccgatcaggc tgcagacaaa ggtcagtgtg aattcggcca
     8101 gtcttgattt tttatacagg tgattatagg tgccggcaag cgtatacaac atcagccagc
     8161 ccgctggcac tatcaggaaa atcagggtcc aggacgtctg gctgatcggg taagctccgg
     8221 catcattgag catccagcta cgccagtgat aataacaaaa ccaggcaagt gcggcagtga
     8281 gataatcggc tgccacatac catcctgctg atatttgttt gccggactgc attaatgggc
     8341 gatcacattg tggccgattt tttcgaggat ctggtgggcc acatccatgg ccatcagtcc
     8401 atccatttca tttactacgg tccggctgtt atttttaatg gcatccctga attcttccag
     8461 ttccagttta atagcgttta cctcaggtac agccggattg gccatggcga tcgttttttt
     8521 gcccgaaggg gtttcaatat cgaaagcgaa aacattgagg tcggtctctt ccttgagttt
     8581 gatgatttct gttttcttat tgaggaagtc gatgccgata taggagtctt tctggaagag
     8641 ccgcactttc cgcatctttt tcatggagat acggctgctg gtcaggttgg ccacacatcc
     8701 attatggaat tcgatccgca cgttggcaat atccggagtt tcggtcatta cggctacgcc
     8761 gctggcagaa atggatttca catcactttt tacaatactc aggatgatat cgatatcgtg
     8821 gatcatcagg tcgaggatca cgctcacttc agtgccccgg ggattaaact gggccaggcg
     8881 gtgaacttcg atgaacatgg gtttcaggtc catgtccttc agggcaagga aggcggggtt
     8941 gaagcgttcc acatgtccca cctggaattt gataccggat tcagcagcca gtttcaccag
     9001 ctgccgggct tcctccatcg tattagccag tggtttttcc acgaatacgt gtttcccttt
     9061 tttaatggcc atttcgcacc attcaaagtg aaaattggtg ggcgccacga tatcaacggc
     9121 gtcgcaggat tcgatcaggg cctccgcatc caggaagcgg gggagctggt atttttcggc
     9181 tacttctttt gcattttcat catggggatc ataaaagcca acgatgtcca ccccggcaat
     9241 ttctttccag ttattcaggt gaaattttcc caggtgacct acgccaataa cgccaatctt
     9301 aagcataac
//
LOCUS       DAOPMW010000097         9255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8162_length_9255_cov_6.793261, whole genome shotgun sequence.
ACCESSION   DAOPMW010000097 DAOPMW010000000
VERSION     DAOPMW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_9255_cov_6.793261"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..2127)
                     /locus_tag="PLU11_06515"
     CDS             complement(34..2127)
                     /locus_tag="PLU11_06515"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58805.1"
                     /translation="MSIFTFLALLGPLKLFAQTDAHWYFGIKAALNFNPSGNQPVPIV
                     LSNNIMETAEASATISDNNGNLLFYTNGVTVFNRNHEIMGNGDGLNGNPSACQIAVIP
                     QPGSDKIFYIFTTDAFEHDFLNGYSYSVVDMSFDGGFGGVVSKNNPLWASCTERITAI
                     RHANGTDVWIITNDENSNIFRAWLLTCNGFNAANPPIISVAGDILDSHPLMNVGVMKG
                     SPDGRFICQTHFPFSDANSAASDFAQLFDFDNSTGMISNARKLSFPATQYNHCEFSPD
                     SKKLYLTRKNNKQLDQLDISQPTFPAILASRVSFPTTTSYYDIQLAMDEKIYLTQANS
                     QLARINFPNEPGAACNFQRNAINVSPGSVFVGLPSHINDIVGSNNQGNGFAYSILDSC
                     TGLVQFNATTSLPGNITWLWEFGDNTSSGLQNPIHLFANPTELHTVKLTVTATGSCGN
                     LIRTRIIRPSGLIKPVANFTHTFVCDSNYIRFINTSLDTTSPGIRFSWDFGDGNFSSQ
                     SNPVHTYQLDGNYTVKLRINTGNPCKNDSISMPVSFSRFSISVPPDQTINYGQTVSLN
                     TNVPADSYVWSPGKWLSDSTGRNPVAMPYDNISYILNAKSGTCTASDTINITVIQNDF
                     LFIPTGFTPNGDGKNDLIHPLINGRYTLKEFSIYNRSGERVFTTSIQGEGWDGRIRGA
                     LQTSGVYIWVFVKRP"
     gene            2259..5735
                     /locus_tag="PLU11_06520"
     CDS             2259..5735
                     /locus_tag="PLU11_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58806.1"
                     /translation="MKQFGKPYLFLLFVLSSFCGYSQLPGPSLPASNLRVKSFKTGTD
                     SLILDTLSIVPGTLLVSRIPVTDYRLDPVNAVLYWINKPSLDSITITYRVFSYKLNSV
                     TKRLNYDSVMNNFYVKPFEFNRGLSNEQRGVFDFGSLKAEGSLGRQIGFGNRQDAVVN
                     SSLNLQLSGMLGDSIEIQAAITDNNIPIQPDGNTQQLNEFDQVYLQFKKRGWQLNLGD
                     IDIRQNQAYFLNFYKRMQGIAFQNQQRISKNTVSKTLVSGSVAKGKFTRQLLEAQEGN
                     QGPYRLKGPNNELFFVVLANTERVYYDGQLLQRGEDQDYVINYNTAEISFTPKRMITK
                     DSRIQVEFEYAERNYLNTNLYLSQEFEINKKWKLRLGAFNNADSRNSQINQILDTRQK
                     QFLADLGDSTRKAYYPTALIDTFAADKILYEKIYAGIDSFYRYSTDPLTARYNLSFVD
                     LEQGNGDYLPDFNGANGKVYKYVAPVAGIKQGRYEPVQILVAPRRQQILNLGVDYAPG
                     KNTLLKAEIATSNNDVNTFSSKDNGDDRGWAAKIQLSNTKRLKAAKELDLTSSLDYET
                     VQKKFSPLERLRNVEFTRDWGLELQSVPLAVDENIIKAGLGLKDKKAHAVSYQLIHYD
                     RSDAYKGLQHQVKHAADWKGWRFNNQLVVTGFDYNQNKGSFLRPTIDLSKQLKKLDNW
                     RAGFNYSLEKSNTVYKLSDTLTPTSFYFDVYSAYLKSDESRKDKYAVTFFTRSDKYAA
                     GKEFIRGDRSLNLNLQTMLEGNAKRQFYLNATFRKLKVLNNTVSTQQEDETILGRAEY
                     VMNEWKGFLTGNVLYEVGAGQEQRRDFAYLEVPAGTGQYAWNDYNSDNIQQLNEFELA
                     AFADQARFIRVFTPTNDFIKANYNTFNYRIDLNPRVLLTDSKLKGMKKFVSRLNLLSS
                     FQTSKKSVATGSFEFNPFKYSVSDTALITLSTSILNTLSFNRFNSKWGLDLTNFRNTG
                     KSLLTYGYESRKVNDWTAKWRWNINRKLALTMSSRTGINALYTPSFANRNYELSLFSA
                     EPTLIFIKGTKFNIKTAYKFDRKKNKPAYGGEKSISHSINLESRYNILQRSSVTAKFT
                     LNNINYNAVANTTVSYIMLDGLLPGSNYLWTIGFNKRLINNLELTFNYDGRKSGAART
                     VHLGRAGVTALF"
     gene            complement(6150..7247)
                     /gene="ychF"
                     /locus_tag="PLU11_06525"
     CDS             complement(6150..7247)
                     /gene="ychF"
                     /locus_tag="PLU11_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPN58807.1"
                     /translation="MALQAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIDPNVGL
                     VDVPDPRLDKLAELVLPNRTVPTQIEIVDIAGLVRGASKGEGLGNKFLGNIREVDAII
                     HVIRCFEDENILRDEGAINPVSDKEIIDTELQLKDLESVEKKIQRNEKLAKLDPKLKA
                     ELEVLQLCKKHLEQGKSIRELDFSKEDRNAIADLFLLTEKPVLYVANVDEASMHTGNK
                     FSEALKEAVKNEKAEVIVMNNNIEAQISEMDNPEDKQLFMEEYKMEEPALDRLIHSAY
                     RLLNLFTYFTAGVQEVRAWTIHQGWRAPQAASVIHTDFEKGFIKAEVIGYDDFINYGS
                     EAACREAGRLRIEGKEYIVKDGDVMHFRFNV"
     gene            7556..8956
                     /locus_tag="PLU11_06530"
     CDS             7556..8956
                     /locus_tag="PLU11_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPN58808.1"
                     /translation="MALSRIWTAFIFIALLVAGGKFLFEPGQEQIFSNLVTGKNTDTV
                     SARSLDSAAIPATVHAQLTQLKLAIWDKEKVAKTSDGSFRVFRILPANGVFETTRDAV
                     NLCIGLIGIMALFMGFMNIAERAGGIRFLSRIIGPFFSKIFPEVPKNHPSMGHMVMNF
                     SANLMGLDNAATPFGIKAMESLQEINPDKNTASNSQLMFLCLHASGLTLIPTIIIADR
                     VALKSQHPTDIFIPCMIATFAATVAALMIVAYRQRIRLFQTVILLWVLGLFAVIAGLI
                     VYIRTLDARGVQLFSTSLSNGIILLIFLLIVAGAVYKKTPVFDAFVDGAKGGFETAVR
                     IIPYLVGLLVAISLLRNSGAFEYVMDALKSLFVWFGGDTRVVDALPTAIIKPLSGSGA
                     RAMMIDTLNAQGVDSFAGNLASVFRGSSDTTFYIIAVYFGAVGIKNTRYAVSTMLLAD
                     LVGIITAISVAYLFFA"
     gene            complement(9048..>9255)
                     /locus_tag="PLU11_06535"
     CDS             complement(9048..>9255)
                     /locus_tag="PLU11_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58809.1"
                     /translation="VAVTDVTSFVPYPSALLTDTLYIRPTGNTTTNLVKYVLTGGLTQ
                     KRSYTLVYRGSDRGTKIANLFANY"
BASE COUNT         2473 a         2058 c         2239 g         2485 t
ORIGIN      
        1 cctccattca gggtgagggg tgggacggca gaatcagggg cgctttacaa acacccagat
       61 atagaccccg gaagtttgta aagcacccct gattctgccg tcccacccct caccctgaat
      121 ggaggtcgta aagactcttt ccccactccg gttatagatg ctgaattctt taagggtata
      181 acgcccatta ataagcggat gtatcagatc attttttcca tcgccattcg gcgtaaagcc
      241 ggttggtata aaaagaaaat cattctgtat aacggttatg ttaatagtat cggatgcggt
      301 gcaggttccg cttttcgcgt tcagtatata actgatatta tcgtagggca tagctacagg
      361 gttacgaccc gtactgtcgc tcagccattt tccgggtgac cacacataac tgtccgcagg
      421 tacattggta ttcaatgata cagtctggcc gtaattaata gtctgatcag gagggacact
      481 gatactgaac cggctgaaac tgaccggcat actgatcgaa tcatttttac acggattacc
      541 ggtattgatc ctgagtttta ccgtataatt tccatccagc tggtaagtat gaaccgggtt
      601 cgactggctg gagaaattgc catccccgaa atcccaacta aagcgaatac ccggtgacgt
      661 ggtatcgagg ctggtattga taaaacggat atagttggaa tcacaaacaa atgtatgggt
      721 gaaattggca acaggcttaa tcagtcctga aggccggata atacgggtcc ggatcaggtt
      781 gccacaggaa ccagtggccg taacagtcag ttttacggtg tgcaactcgg ttgggttggc
      841 aaataaatgg atcggatttt gcaaaccgga actggtattg tccccgaatt cccatagcca
      901 ggtaatattt cctggcagcg aagtggtggc attaaactga accaggccgg tacaactgtc
      961 caggatggaa taagcaaatc cattcccctg gttattggaa ccgactatat cgttaatgtg
     1021 agagggtaaa cctacaaaaa cagaaccggg gctgacattg atcgcgttgc gttggaaatt
     1081 acaggcagct cctggttcat tggggaagtt gattctggcc agttgtgaat tagcctgtgt
     1141 aagataaatt ttttcgtcca ttgccagttg aatatcataa taactggtgg tggttggaaa
     1201 acttaccctg gaagccagaa tggccggaaa tgtgggctga ctgatatcca gctgatccag
     1261 ctgtttatta tttttccggg tcaggtaaag ctttttggag tccggtgaaa attcacaatg
     1321 gttatattgc gtggccggga aactgagttt tcgggcatta ctaatcatac cggtgctgtt
     1381 atcaaaatcg aaaagttgtg caaaatcaga agcagcggaa ttggcatccg aaaaaggaaa
     1441 atgggtctgg caaataaacc gtccatcagg acttcccttc ataacaccaa cgttcatcag
     1501 cggatgtgaa tctaaaatat ctcctgcaac agatatgatg ggtggatttg ccgcattgaa
     1561 tccattacaa gttaacagcc aggccctgaa aatatttgaa ttttcatcat tcgtgattat
     1621 ccatacatca gtaccattcg catgacggat ggcggtaatc cgttccgtac aggaagccca
     1681 gagcggattg tttttgctta caactcctcc aaaaccacca tcgaagctca tgtccacaac
     1741 agagtaagaa taaccattca gaaaatcatg ttcaaatgca tcagtggtaa agatgtaaaa
     1801 aatcttatcg gatccgggtt gaggaattac ggctatttga caggctgacg gattgccatt
     1861 caggccatcc ccgttgccca ttatttcatg attccggtta aaaacagtaa cgccgttggt
     1921 atagaaaagc aggttgccat tattatcgga aatggtggca gaggcttctg ccgtttccat
     1981 tatattatta cttagaacga ttgggacagg ctgatttccg gatggattaa aattgagagc
     2041 tgctttgatt ccaaaatacc aatgggcatc tgtctgggca aacagcttta aagggccaag
     2101 cagggccaga aatgtgaaaa tgctcaaaat tgtcaacctc acagggggca tggccatact
     2161 tatctgattg attatcaaaa ttaagtagtt ttacaggaag agacgaatcc agacaataaa
     2221 aacatccatt tttacgcttt tgtgttaaac cgctgctttt gaagcaattt ggaaaaccat
     2281 atcttttttt actattcgta ttgtcttcat tctgcggcta ttcacagttg cctggccctt
     2341 cattaccggc ttccaatctg cgtgttaagt cttttaaaac cgggacagac agtctgatcc
     2401 tggataccct tagtatcgta cccggcaccc ttttggtttc ccgtatacct gtaaccgatt
     2461 accggctgga tccggtgaat gcggttttgt actggatcaa caaacccagc ctggattcga
     2521 taactatcac gtaccgggtt ttttcctaca aactgaacag tgtcaccaaa agactgaact
     2581 atgacagcgt gatgaacaat ttctatgtga agcctttcga attcaaccgg ggattgtcca
     2641 atgaacaaag gggcgtattc gatttcggtt ccttaaaagc cgagggcagc ctgggccgtc
     2701 agatcggttt tgggaaccgg caggatgcgg tagtgaactc ctccctgaat ctgcaactca
     2761 gcggtatgct cggtgacagt atcgaaatcc aggccgcgat caccgataat aatattccga
     2821 ttcagccgga tgggaatacc cagcaattaa atgaattcga ccaggtttat cttcagttta
     2881 agaagcgagg ctggcagctc aacctgggtg atattgatat ccgccagaac caggcttatt
     2941 tcctgaattt ttataaacgg atgcagggga ttgcctttca gaaccagcaa cggatttcaa
     3001 aaaacacggt atccaaaaca ctggtcagcg gttcagtggc caaaggaaag ttcacccgtc
     3061 agctgctcga ggctcaggag ggtaaccagg gaccttaccg gctgaaaggg ccaaataatg
     3121 agctcttctt tgtggtgctt gccaatacgg aacgggtgta ttatgacggg cagttattgc
     3181 agcggggcga agaccaggat tatgtgatca attataatac tgcggaaatt tcgtttaccc
     3241 ctaagcgtat gatcacgaag gacagccgta tccaggtgga atttgaatat gcggaaagga
     3301 actacctcaa tacgaatctg tacctgtcac aggaatttga aatcaataaa aaatggaagc
     3361 tccggctcgg ggccttcaac aatgcggatt cgcgtaattc acagatcaac cagatactgg
     3421 atacccggca gaaacagttc ctcgccgacc tgggtgacag cacccggaaa gcgtattatc
     3481 ccaccgccct gatcgatacg tttgccgcag ataaaatcct ctatgaaaaa atttatgcag
     3541 gcatcgattc tttttaccgc tattccactg atccgttaac cgcccggtat aatctttctt
     3601 ttgtggacct ggagcagggg aatggtgatt atctgcctga ctttaacggg gccaatggaa
     3661 aagtgtacaa gtatgtggct ccggtggccg ggattaaaca gggacgctat gaaccagtac
     3721 agatactggt ggcgcccagg cgtcaacaga ttctcaacct gggcgtggac tatgcacctg
     3781 gaaagaatac tttattgaaa gcagagattg cgaccagtaa taacgacgtc aatacttttt
     3841 cctcaaaaga taacggggat gaccggggct gggcggccaa aatacaactc agtaatacga
     3901 aaagactgaa agccgccaaa gaactggatc tgaccagtag cctggattat gaaaccgtac
     3961 agaaaaaatt cagtccgctg gaacggctgc gtaatgtgga attcacccgt gactggggac
     4021 tggaattaca atccgttccg ctggctgtgg atgaaaatat aatcaaagcg ggactgggat
     4081 tgaaagataa gaaagcgcat gccgtcagtt accagctgat tcattatgat cgaagtgatg
     4141 cttacaaagg acttcagcac caggttaagc atgcagctga ctggaaaggc tggcggttta
     4201 ataaccagct ggtggtaacg gggtttgatt ataatcagaa taagggaagt tttctccgac
     4261 caaccattga cctcagcaaa caacttaaaa aactggacaa ctggagagcc gggtttaact
     4321 attcactgga gaaaagtaat acggtctata agttatctga taccctgaca cccacttctt
     4381 tttattttga tgtgtattcc gcttatttga aatcggacga aagccggaag gataaatatg
     4441 cggttacttt tttcacccgg tctgataagt acgcagcggg taaggagttt atccggggcg
     4501 atcgcagtct gaacctgaat ctccagacca tgctggaagg aaatgccaaa cgacagtttt
     4561 acctgaatgc tactttccgg aaactgaaag tgctgaacaa tacggtttcc acccagcagg
     4621 aagatgaaac tattttaggc agggcggagt atgtaatgaa tgaatggaaa ggcttcctga
     4681 ccggaaatgt gttatatgaa gtaggagcag ggcaggagca acgacgggat ttcgcctatc
     4741 tcgaagtgcc ggcaggaacc ggacagtatg cctggaatga ttataacagt gacaatatcc
     4801 agcagttgaa tgaatttgaa ctggctgcct ttgccgacca ggcaagattt atccgggttt
     4861 tcacaccgac caatgatttt atcaaggcca attataacac gtttaactac cggattgatt
     4921 taaatccccg ggtattgctg acggattcaa aactgaaggg gatgaagaag tttgtttcca
     4981 gactaaacct gttaagttcc ttccagacca gtaaaaaatc cgtagctacc ggttcatttg
     5041 aattcaatcc gtttaagtac tccgttagtg atacggccct gataaccctg agcaccagta
     5101 ttctcaatac gctttctttt aaccgcttca acagcaaatg gggactggac ctgacaaatt
     5161 tccggaatac cggtaaatcc ctgctgacgt acggatacga gagccgtaag gtcaatgact
     5221 ggacagcaaa atggcgctgg aatattaacc ggaaactggc tttgaccatg agcagccgga
     5281 ccgggatcaa tgcgttgtat acgccttcct ttgccaaccg gaattacgag ttatccctgt
     5341 tcagtgcaga gcccaccctg atctttataa agggcaccaa attcaatatc aaaacggcgt
     5401 ataagtttga ccggaagaaa aataaaccgg catatggcgg ggagaaatcc atctctcatt
     5461 ccatcaacct ggaatcaagg tataatatcc tgcaaaggag ttcggtgacg gcgaagttta
     5521 ccctgaataa tattaattat aatgcggtgg ccaacaccac ggtcagctat atcatgctgg
     5581 atggtttatt acccggcagt aattatctct ggaccatcgg gtttaataaa cgactcatca
     5641 ataacctgga acttactttc aattatgatg ggcgtaaatc cggcgcggcg agaacggtgc
     5701 accttggccg ggctggtgtt acggctttat tttgaggcgg ccccctaatc ccccgaaggg
     5761 ggacttcaaa ctccgatatt agtaaagtca tttactgttt tgcattgcgt aatgcgtccc
     5821 gcaaagccaa aaaagacttt tccttaatcg gagtgtgttt aaatcttagc gggaaatctc
     5881 acgcaagact cggcccccta aatcccccga agggggactt cagactcctt ttatagtaaa
     5941 gtcaattagt gttttgtatt gcgtaaatat gtcccgcaaa gccaaaaaag acttttcgtt
     6001 gaacgtagag acttataaat cttagcggga aatcctacgc aagtctaaga gaaaggaaag
     6061 ttcaaactat acgggtcttc gaagtccccc ttcgggggac ctgcctgccg gacaggcagg
     6121 tttagggggc cgtatttagg gggccgcctt tacacattaa acctaaaatg catcacatct
     6181 ccatccttca caatgtattc tttcccttca atccgtaagc gaccggcttc gcggcaggcg
     6241 gcttctgaac cgtagttgat aaagtcatcg tatccgatta cttcggcctt gatgaatcct
     6301 ttttcaaagt cggtatgaat cacgctcgcg gcctgcggag ctctccatcc ctggtggatg
     6361 gtccaggccc gtacttcctg aacaccggcg gtgaagtaag taaataaatt cagtaagcgg
     6421 taagcggagt ggatcaggcg gtcgagggcc ggctcttcca tcttatattc ttccatgaag
     6481 agctgtttgt cttccggatt atccatttca gaaatctgtg cttcgatgtt attgttcatc
     6541 acgatcactt cagccttttc attcttcacg gcttctttca gggcttcaga gaatttatta
     6601 ccggtatgca tggacgcttc gtccacgttg gctacataga gtaccggttt ttcggtgagc
     6661 aggaacaggt cggcaatggc gttacggtct tcttttgaaa aatcaagttc gcggatgctc
     6721 tttccttgtt ccaggtgttt tttacataat tgcaatactt ccagttctgc tttcagtttg
     6781 ggatccagct tagccagctt ctcattacgt tggatcttct tctccacgct ttcgagatct
     6841 ttcagctgca gttcggtatc tatgatttcc ttatcactga ccggattgat cgcgccttca
     6901 tcacgcagaa tgttttcgtc ttcaaaacaa cggatcacat ggatgatggc atccacttcc
     6961 cggatatttc caaggaattt attgccaagg ccttcgcctt tactggcacc acgcaccaat
     7021 ccggcaatat ccacgatctc gatctgggtg ggaacggtac ggttgggaag caccagctcg
     7081 gccagtttat ccagccgggg atccggtaca tcgaccagtc ccacattggg gtcgatggta
     7141 caaaaacgat aattactcgc ctgtgctttg gcgctgttgc tgacggcgtt gaacaaagtt
     7201 gatttaccga cgtttgggag acccacgata cctgcctgta atgccatgag gagttgtaag
     7261 ttttaagtta taagttttaa gtttgagtcc gaaagaacaa agaccgggag tccgtaagaa
     7321 agaagtgttt cgaacttccg ggcttttccg acttcctgtt attcggactt cttaaaaagt
     7381 gtgcaaagat agcttaattc ttcggcatgg cctcctccta atgccgctaa atctcgttat
     7441 cttcatcatg tcataaacgg cttgttaaca acatcttgta gaatcagtgt attgacctgc
     7501 ctttgtcggc aggcaggctt aaaacttact acttataact tataactaga tatttatggc
     7561 tttaagcaga atctggaccg ccttcatatt catcgcatta ctcgtggctg gaggcaaatt
     7621 tctttttgaa cccgggcagg agcagatttt cagtaacctg gtaaccggaa aaaatacgga
     7681 tacggtttct gccagaagtc tggattctgc cgctattccg gcaacggtac atgcccagct
     7741 gacacaactg aaactggcga tctgggacaa agagaaagtg gcaaagacca gtgatgggtc
     7801 attccgggtg ttcagaatat tgccagccaa cggggttttc gaaaccaccc gtgatgcggt
     7861 gaatctttgt atcggactga ttggaattat ggcgctgttt atgggcttta tgaatattgc
     7921 agaaagggca ggagggatcc gttttttatc acggatcatt ggtccgttct tttcgaagat
     7981 atttccggag gttcccaaaa accacccttc aatgggtcat atggtgatga atttttccgc
     8041 gaacctgatg ggactggata atgcggcgac tccttttggc attaaggcga tggagagcct
     8101 gcaggagatc aacccggaca aaaacacggc gtccaattca cagcttatgt ttctctgcct
     8161 gcatgcttct ggtctgaccc tgatccctac gatcattata gcggaccggg tggcgctgaa
     8221 atcgcagcac ccaacggata tcttcattcc ctgtatgatc gccaccttcg ccgctacagt
     8281 ggctgccctg atgattgtag cgtatcgtca gcggatccgg ctttttcaga cggttatcct
     8341 tttatgggta ttgggattat ttgccgtaat cgccggcctt attgtttata tcagaaccct
     8401 ggatgcgaga ggggtgcaat tgttttccac cagcctgagt aacggtatta ttctcctgat
     8461 cttcctgttg attgtagccg gggcggtgta taagaaaaca ccggtttttg atgcctttgt
     8521 ggatggggcg aagggcggct ttgagaccgc tgtgcggatc attccgtatc tcgtgggatt
     8581 gctggtagcg atcagcctgt tgcgcaacag cggggcattt gagtatgtga tggatgcatt
     8641 gaaatcattg tttgtctggt ttggcggaga tacccgggtg gtggatgcgt tgccaaccgc
     8701 gatcatcaaa ccgctcagtg ggagtggggc aagggcgatg atgattgata ccttaaatgc
     8761 gcagggggtg gattcctttg ccggtaacct ggcctccgta ttccggggat catccgatac
     8821 gactttttat attatcgccg tttattttgg cgcggtaggg attaaaaaca cccgttatgc
     8881 ggtcagcacc atgttgctgg ctgacctggt ggggatcatt actgcgatat ccgttgctta
     8941 cctgttcttt gcctgattca tccagaggga ggcagatatg cgaactgata aaaaaaaaga
     9001 ggctgtcttt tcagatagcc tcattattat aaagggaatt gttatgctta ataattcgcg
     9061 aacaggttgg cgatttttgt gcctctgtcc gatccgcgat agaccagggt ataactcctt
     9121 ttctgagtaa gtccgccggt caggacgtat ttaaccagat tggtggtggt atttccggta
     9181 ggccggatat acagtgtatc cgtcagcaga gctgaaggat acggtacaaa actggtcaca
     9241 tctgttactg caaca
//
LOCUS       DAOPMW010000098         9253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8166_length_9253_cov_8.288758, whole genome shotgun sequence.
ACCESSION   DAOPMW010000098 DAOPMW010000000
VERSION     DAOPMW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8166_length_9253_cov_8.288758"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4604)
                     /locus_tag="PLU11_06540"
     CDS             complement(<1..4604)
                     /locus_tag="PLU11_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN58810.1"
                     /translation="MNFISKGPLRLICIVFLFGATLPAAVLQAQTSISDYVLFGGKQA
                     AGQTTPSAPGYGVLIGTSCHLQATIQGAGLGSVGSYNLVKTTGSLTLNGNIYSGGTIQ
                     LFSGNVVKGNLSAGLSPVGVPGNVIATGTGTILGVSGNPLTGNINANGKVTVAGGTIY
                     GNVTLPAPSSVYPYIGPAPAGGVVYGSPELPGLPAMPPITDFPNFGTTNISNTATIAP
                     GVYGNVNLGNNKKLTLSGPGVYVFKSFTTTGPNSSIVYNFNNTATGDFKIYIHGDVRL
                     NKAGFSTINGGSANRIYVETHGTGSTNPYDHTAAFYMSNGSNGSGNPSGWLGTLWAPY
                     AAIKIGAPNGPSSSIKGALWSGTQINLQGGVTVNFAPYNLCTPPNANAGPDKALDFEN
                     PTTLTGTSGTSGVTYNWQAINGGVISTAPDAATITVNSAGTYVLTVTSNPGCSSTDTV
                     IVTGKVSDIIGSELQAVVQNNGTPSPFFDIQNGKIFIDVIVKAGEYSYVKGKLTATGG
                     PWQMTDLLGEDASQLIITGLFPINKLLSLNTDPDLSPRIVYARPYYRAFTNYSNITAG
                     LTRTQGDTTIKADLVRLGYNLSGEGIKIGVLSNSFGTIASASSNPVNSTAADDVREKD
                     LPGAGNPNNENPVTVLKESRFVRSDEGRAMLQIIHDIAPKAQLYFRTGIETANDFADG
                     IRELKAAGCNIITDDITYITEPFLKDGVVANAVGEVTAAGATYISAAGNFAKKSFDGV
                     FTPMTAPGNLGGKAHDLGSGNPFLNVTLVPGQQYTIVLQWLDSIYSLGETASNGTKND
                     LDIYLTPNTDGTALFGFNRDNLNGDPIEFLPFIYSGPASTHMLVVNNTVNDGSNPPPR
                     FKIVVFQGDITFNGYSGGSTIIGQSNADSAITVGAARFDRKATLQAFSSIGGTVVNNI
                     VRNKPDLVAPDGVNTTVNMGIDQPNDPDTEYSNFFGTSAAAPHVAGVAALLMEGKKKF
                     LNQSSVSAYEIKTLLKSTATPLGSSPGYNFASGAGFINADAAMRSFAAPDPSLIQLVV
                     PPNTIPGSSPFTLTITGLNLSPTSVVRFRDSSLTLLYLSAGRDTATAEVPAFVGDPAI
                     SVYTPPLFSTGIDGGTSDTLKFFDVVKKNVVVKAENKTKKYAQSFPEFTYTVTVDGVA
                     LADLPAPGLTTQQLGLDQLTLTSSANNDILSNVGTYSITPNRVFSETDPVDIGMNELY
                     NYTFTNGTLTIGKLPVTVTARDTTVTYLQKIPDIQFNYALTNAADGANIPSVGTVLDA
                     IQTTHQSQLAKDGSGKDVLGLVNGQAVTIVNGQAIPIVNGQAVTIVNGQAVTIVNGQA
                     IPIVNGQAVTIVNGVVTDTIPEVLTAPEINQLSFQAGPQSLQSTRTIQNKELVNGSYI
                     TSSTNVVDITQESILDFNNNAAQTTMLNAISGVRSKGLVAQESYVNGQAITIVNGQAV
                     TIVNGQAVTIVNGQAVTIVNGQAVTIVNGQAIPIVNSEERTAVVLDTTEIGQGVNPLK
                     SLNMVTGLDAGQQYVIPGSFVNPNFEITHLAGVVTILPAPV"
     gene            5037..5606
                     /locus_tag="PLU11_06545"
     CDS             5037..5606
                     /locus_tag="PLU11_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPN58811.1"
                     /translation="MYYFWKFYFKLIGWKLREPFPGPLPKAVLAVGPHTSWQDFIIGV
                     ATRSLMRIGPIYFLGKKELFDGPFGWLFRALDGKPVDRFSNKGMVEQVAELFSKDPNF
                     KIALAPEGTRKRVDKLRTGFYHIAEKAGVPIVLVGFDFKTKQVCFSNPLYPSNEEQDM
                     KTIHNFYGSMTGKFPEKDLQHLGKLEASI"
     gene            complement(5685..6467)
                     /locus_tag="PLU11_06550"
     CDS             complement(5685..6467)
                     /locus_tag="PLU11_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPN58812.1"
                     /translation="MKLTDTHAHIYLPEFAETRKEMLERAEKEGVSNILLPAIDSESH
                     AALLELARQYPGQCRPMMGLHPCYVKEHFREELKIARAYLEKGGFVAVGEIGLDFYWD
                     KTFTEQQYLAFEEQIHWALEFDLPIAIHSRNSTDECIEVVRRNQNGKLKGVFHCFSGN
                     TVQAQQIMDLGFYLGIGGVVTFKNGGLDKVFDTISLDKVVLETDAPYLAPVPFRGKQN
                     EPSYLKYVVEKLAGLKNISIEEVAEITERNVRELFSKLAAKS"
     gene            6585..7487
                     /locus_tag="PLU11_06555"
     CDS             6585..7487
                     /locus_tag="PLU11_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58813.1"
                     /translation="MKLRLIKAVYIIISFLFTSFIFTSCQKEQSQSEQDAQEVEASRI
                     SGESESEAETVFNGVFDDAMGVNDEVGIGGTGVFGRLNVCPTVTITRPNLPDLFPVRV
                     EMDFGANGCIGPDGHFRKGKVITVYTKRLLIPGAVAETSFDGFYVDSVHVEGTHKITN
                     ISTVVTAGTPPVDRKFRVVIINGKLTKPNGNYISWNSEKTISQVEGLGTILPMDDIFK
                     IEGGSRGQVKRGNLLVGFNSNITEPLMKRFNCRWIAKGIVKTVRLNASTNSPWVATLN
                     FGIGNCDNLAVLTINGVSYQITLP"
     gene            complement(7574..8212)
                     /locus_tag="PLU11_06560"
     CDS             complement(7574..8212)
                     /locus_tag="PLU11_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPN58814.1"
                     /translation="MRNYLIKTPWWLKRLYPRRIWQMDTSAKKIYLSFDDGPHPIATP
                     FVLDTLKKYEAKATFFCLGKNVAAYPEIFQRIVAEGHSVGNHTQDHLNGWFTKTETYL
                     RNVGLAKRYIQSPLFRPPYGKIRSAQVKKMHHYRIIMWDVLSGDFDKSVSAENCFENV
                     ARHTNNGSIVVFHDSEKAWPKMSNTLPLVLEHFSKKGFEFSAILGGKNDRLR"
     gene            8274..>9253
                     /gene="gltX"
                     /locus_tag="PLU11_06565"
     CDS             8274..>9253
                     /gene="gltX"
                     /locus_tag="PLU11_06565"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPN58815.1"
                     /translation="MNKRVRVRFAPSPTGGLHLGGVRTVLFNYLFARKHGGDFLVRIE
                     DTDQNRYVPGAEEYIFDTLKWCGLEPDESVQHGGNYGPYRQSERKASYRIFAEQLVNA
                     GHAYYAFDTPEELETMRQEYKTPENPSPQYDHSIRMKMRNSLSLTAEETAQLLANGTR
                     HVIRIKMPENETVSFTDMIRGEVSFESGTVDDKVLLKADGMPTYHLAVVVDDQAMEIS
                     HAFRGEEWLPSAPVHILLWKYLFGLDQMPQWAHFPLILGPSGKLSKRDGAKYGFPVFA
                     MNWTDPKTGELTEGFKEKGFLPEAFINLLALLGWNEGTEQEIYTKEELIQ"
BASE COUNT         2419 a         2162 c         2181 g         2491 t
ORIGIN      
        1 acaggcgctg gtaaaatggt caccactcct gcgaggtgcg tgatttcaaa attaggattg
       61 acaaatgatc ccggaatcac atactgctgt ccggcatcga gtccggttac catattcagt
      121 gatttcagcg gattgacacc ctgtcctatt tcggttgtat ccagcaccac ggctgttctt
      181 tcttcactgt ttacaatagg tattgcctgt ccattgacaa tcgtcaccgc ctggccattc
      241 acaattgtta ccgcctggcc atttacaatc gtcaccgcct ggccattcac aatcgttacc
      301 gcctgaccat tcacaatcgt tattgcctga ccattcacat aagattcctg ggccaccaat
      361 ccttttgatc gtacaccgga tatggcattc agcattgtgg tttgtgcggc attattgttg
      421 aagtccagaa tagattcctg ggtgatatcc accacattgg tgctactggt aatataagaa
      481 ccattgacta attctttatt ttgtatcgta cgggtcgatt gcagggattg aggtccggcc
      541 tggaaactaa gctggttgat ttccggagcg gttaagactt cgggaatcgt atcggtgact
      601 acgccattta caatggtaac ggcctggcca ttcacaatcg gaatggcttg tccgtttaca
      661 atggtgaccg cctggccatt cacaatggta actgcctgac catttacaat cggaatggct
      721 tgtccgttta caatggtgac cgcctggcca tttacgagac ctaatacatc tttgcccgaa
      781 ccatctttgg ccagttgtga ctggtgcgtg gtttgaatgg catctaatac cgtacccaca
      841 ctggggatat tggccccgtc agccgcattg gtcagtgcat aattaaattg aatatccggt
      901 attttctgca ggtaagtgac cgtggtatca cgggccgtta cggttaccgg cagttttccg
      961 atggtcagtg taccattggt gaatgtataa ttatacaatt cattcattcc gatatccacc
     1021 ggatcggttt cactgaatac ccggttgggg gtaatcgaat aggttcctac attacttaaa
     1081 atatcattgt tggcagagga agtcagggtc agctgatcca gccccaattg ctgcgtggtt
     1141 aatccgggtg ctggcaaatc agccagggcc acgccatcca ccgtcacagt gtaggtaaat
     1201 tcagggaagg actgggcata cttcttggtt ttgttttccg ctttcaccac cacattcttt
     1261 ttaacgacat caaagaattt gagtgtatca ctcgtgcccc cgtcgatacc ggtagaaaat
     1321 aacggtggtg tatacacgga gatggccgga tcgcccacaa atgccggcac ttcagccgtt
     1381 gccgtatctc ttccggcact gagatagagt aaggttaggc tggaatcacg gaaacgcact
     1441 accgatgtgg gactcaggtt taatccggtt atggttaagg taaacggact gctgccgggt
     1501 atggtattgg gcggcacgac gagttgaatc aatgatggat ccggtgcggc aaaactgcgc
     1561 atggcggcat ccgcattgat aaagccggct cctgaagcaa aattatagcc agggctgcta
     1621 cccaatggag tggccgtgct tttcagtaag gttttgattt cgtacgcgga taccgacgat
     1681 tgattcagga attttttctt tccttccatt agtaaggccg ctactccggc cacgtgcggg
     1741 gccgccgcgg acgtaccaaa gaagttggaa tactcagtat ccggatcatt gggctgatca
     1801 atgcccatgt ttaccgttgt attcacgcca tcaggtgcca cgagatccgg tttattacgc
     1861 acgatattgt ttactaccgt accaccaatc gaagaaaaag cctgcagggt ggctttacga
     1921 tcaaagcggg ctgcccccac cgtaatagcg ctgtctgcat tggattgtcc gataatggtg
     1981 gaaccaccgc tatatccatt aaaagtgata tccccctgga acaccacgat cttaaaccgg
     2041 ggcggcggat tgctaccatc gttaactgta ttattcacga ccagcatatg tgtggaggca
     2101 ggccctgaat agataaaagg taaaaattca atcggatccc cgttcaggtt atcccggtta
     2161 aatccaaaca aggcagtacc atccgtattg ggtgtgagat aaatatcgag atcatttttg
     2221 gtgccgttgc tggcggtctc tcccagagaa tagatactgt ccagccattg caacacgatc
     2281 gtgtattgct gtccgggaac cagggtcaca ttcaggaacg gattgcctga gccaagatcg
     2341 tgtgctttac cacccaggtt accgggtgcc gtcataggag taaatacgcc atcaaatgat
     2401 tttttggcaa aatttccggc agctgaaata taggtcgcgc cagcggcagt tacttcacct
     2461 acggcattgg ccaccacccc gtctttcagg aagggttcgg tgatatacgt aatatcgtcg
     2521 gtgataatat tacatccggc agcttttaat tcacgtatcc catccgcaaa atcattggcg
     2581 gtttcaatgc cggttctgaa atacaattgg gccttgggag caatatcatg aatgatctgc
     2641 aacatcgccc ttccttcatc tgagcggaca aaacgggatt cctttaatac agttaccggg
     2701 ttctcattat ttggattacc ggcaccggga aggtctttct cccgcacatc atcagcggcc
     2761 gtactattga ccggattgga ggaagcggag gcgatggtac caaagctgtt ggataacacg
     2821 cctatcttga ttccttcccc gctcaggtta tatcccaatc gtaccaggtc tgcttttatc
     2881 gtggtatctc cctgtgttcg ggtcaatccg gctgtgatgt tactatagtt ggtaaaggcg
     2941 cggtaataag gacgggcata aacgatccgg ggggaaagat ccggatccgt attcagactg
     3001 agcagtttgt tgatcgggaa cagtccggta ataatcagct gactggcatc ctcgcccagc
     3061 aggtcggtca tctgccaggg tccaccggtg gcggtgagct tgccttttac ataactgtat
     3121 tctccggcct ttacaattac atcaataaat atttttccat tctgaatatc aaagaaaggg
     3181 gaaggcgttc cgttattctg cactacagcc tgcaattccg aaccgatgat atcacttact
     3241 ttaccggtca cgatcaccgt atcggtggat gagcaacccg gattggaggt tacagtcaag
     3301 acataggtac ctgcggaatt cacggtaata gtagccgcat cgggagcggt gctgatcacc
     3361 ccgccattaa tagcctgcca gttataggtg acccctgaag tacctgaagt accggtcaat
     3421 gtggtcgggt tttcaaaatc cagcgcctta tccggtccgg cattggcatt gggcggtgta
     3481 cagagattat acggggcaaa atttacggtt acacctccct gtaagttgat ctgcgtacca
     3541 ctccagagtg ctcctttaat agaagaggaa ggaccgttgg gagcgcctat ttttatagcg
     3601 gcataaggag cccagagtgt tcctaaccag ccggaaggat taccggatcc attggaaccg
     3661 tttgacatat aaaaggcggc agtatgatca tatggattag tagagcctgt tccatgagtc
     3721 tctacataaa tccggttggc agaaccacca ttgatggtac tgaaaccggc tttattcaac
     3781 cggacatccc cgtgaatata aattttgaaa tcaccggtgg cagtattatt aaaattataa
     3841 acgatactgc tgtttggacc tgtggtggtg aatgatttaa acacataaac acccggaccg
     3901 gataaggtca gttttttatt attccccagg tttacatttc catatacacc gggcgcaatc
     3961 gtagccgtat tggaaatatt tgttgtcccg aaattgggaa aatcagtaat gggcggcatc
     4021 gcaggtagtc ccggtaattc agggctgcca tataccaccc cacctgcggg ggctgggcct
     4081 atatatggat aaacagagga aggagccggc aaagttacat tcccatagat ggtgccacct
     4141 gctacagtga ctttcccgtt tgcatttata ttgccagtta acggatttcc gctgacaccc
     4201 aggattgtac ccgttccggt tgcaatcacg tttcccggta cacccacagg gctgagtccc
     4261 gctgaaagat tccctttcac tacattacca ctgaaaagct gaatggtacc tccactatat
     4321 atatttccat ttaaggtaag tgagcctgtt gttttgacca ggttataact acccacagaa
     4381 ccgagaccag ccccttgaat ggtagcctgc agatgacagg aagtgccgat caacactccg
     4441 tatcccggag cagacggggt ggtttgtccg gccgcctgtt tccctccgaa aagaacataa
     4501 tcagaaatac tggtttgtgc ctgcaaaaca gcagcaggca aagtggcgcc aaataagaat
     4561 acaatacaga taaggcgcaa tggccctttc gaaatgaagt tcatttccgt tggtttttgg
     4621 tggtttgcaa gagaatcaaa gtggttgttc ggtcggataa ggtggatttt caataaacac
     4681 cctttcatat tgaggatggc tcaattgaaa aatgaacatt gctacgcatc aggtagatta
     4741 agtcactgct tttaaatagg aatagtggga atgaggtggg caggaaaaat ttaatctgat
     4801 aagaaacatg gtcttactcc atggtttatt atcgaaccat caagatactg tttttttcta
     4861 tggtcaggta attatttata gtttaccgtt actgtttaac gacagatacc atattggcaa
     4921 acaaatattt aacgagaatt gtccctggca tcatttaatt gaaaattagc agcaaaaaga
     4981 ttgataattg tcaacagctc ctgcatttcc cggaatccag taccttccgt gctattatgt
     5041 attacttctg gaaattctat ttcaaactca tcggctggaa gttacgggaa ccctttcccg
     5101 gccccttacc taaggcagta ttggcggtcg gaccgcacac cagctggcag gattttatta
     5161 tcggggtagc aacaaggagt ctgatgcgga tcggacccat ttacttcctc ggaaaaaaag
     5221 aattgtttga cggtcctttt ggctggctgt tccgggcctt agatggcaaa ccggtggacc
     5281 gcttcagtaa taaagggatg gtggaacagg tggctgaatt attcagtaag gaccctaatt
     5341 tcaagattgc cctggccccg gaaggtaccc gtaaacgggt ggataaactc cgcaccggct
     5401 tttatcatat tgccgaaaag gcgggcgtac cgatcgtact ggtaggattt gattttaaaa
     5461 ccaaacaggt ttgtttttcg aacccattgt atccctccaa tgaagaacag gatatgaaaa
     5521 caatacacaa tttctacggg agcatgacag gaaaatttcc agagaaagat ttacagcact
     5581 tagggaagct agaagctagc atctagaagg aaacagcccc tcctgtgaat ttgtgtctaa
     5641 atgaacaaaa agttcctcgt tttacggcat tccccttctc gcttctagct ttttgctgct
     5701 agcttactaa acaattcccg cacattccgc tccgttatct ctgcaacttc ttcaatagaa
     5761 atgtttttca aaccggccag tttctccacc acatatttca ggtaggatgg ttcgttttgt
     5821 ttcccccgga aaggcaccgg ggcgagataa ggtgcatcgg tttccagtac tactttatcg
     5881 agactgatcg tatcaaacac tttgtcaagc ccgccgtttt taaaggttac gaccccgcca
     5941 attccgagat aaaaaccaag atccataatc tgttgggcct ggaccgtgtt tccggaaaag
     6001 caatgaaaca ccccttttag ctttccgttc tggttgcgtc tgaccacttc aatacactca
     6061 tcggtggagt tccgggagtg gatagcgatg ggcaaatcaa attccagtgc ccaatggatc
     6121 tgttcttcaa atgcgaggta ttgctgttcg gtaaaagtct tgtcccagta aaaatccagc
     6181 ccgatctccc ccacggccac aaatccacct ttttcgaggt aggcgcgggc aattttcaat
     6241 tcctcccgga aatgctcctt gacatagcaa ggatggaggc ccatcatggg tctgcactgg
     6301 ccgggatatt gacgggccag ttccagcagg gcagcatggg attctgagtc gatggccggt
     6361 agcaatatat tactaactcc ctctttttca gctcgttcca gcatttcctt ccgggtttcg
     6421 gcaaattctg gcaggtaaat atgggcgtgg gtgtccgtaa gtttcatgaa aaaaggggtt
     6481 caggccgcaa aactctgcaa aaaactttaa gattccctta aacttttcgg tccgggaacg
     6541 ttcctaaaga agttaacttg tcaacccaaa tcaaaacata gtttatgaaa ctcaggctta
     6601 ttaaggctgt ttacattatt atcagcttcc tttttacctc ttttatcttc acttcctgcc
     6661 agaaagagca gagtcagagt gaacaggatg cccaggaagt ggaagcatcc cgcatctccg
     6721 gagagtcaga atcagaagcg gaaaccgttt tcaatggtgt ttttgacgat gccatgggcg
     6781 taaacgatga ggttggaatt ggtggtaccg gtgtttttgg acgcctgaac gtttgtccaa
     6841 ccgttacgat cacccgcccg aatctccccg atcttttccc ggtccgcgtt gaaatggatt
     6901 tcggtgccaa cggatgtata ggtcctgacg gtcatttccg gaaaggaaaa gttattaccg
     6961 tttatacgaa gcgtttactg atacccggag cggttgccga aaccagtttt gatggctttt
     7021 atgtagatag tgtacatgtg gaaggcacgc ataaaatcac gaatatcagt acggtagtca
     7081 cagccggtac ccccccagtt gatcgtaaat tcagggtggt aataatcaat ggcaaactga
     7141 caaaacccaa tggcaattat atctcatgga acagtgagaa aaccatttcc caggttgaag
     7201 gactgggaac catccttccg atggatgata ttttcaaaat agaaggtgga agccgtggac
     7261 aggttaaaag aggaaatctg ctggttggtt ttaattccaa cattacggaa cctctgatga
     7321 aacgctttaa ttgccgctgg attgccaaag gaatcgtgaa aacagtgcgg ttaaatgcaa
     7381 gcaccaacag cccttgggta gccactttaa acttcggtat cggcaattgt gataacctgg
     7441 cggtcctgac aattaatgga gtttcttacc aaattacttt gccgtaataa aaaaattatt
     7501 atacctttaa accagttttc atagggtacg gattaaaaac gggccagggt ttctacccag
     7561 gccctaactt tttttagcga agcctgtcat tttttcctcc caaaatggca gaaaattcaa
     7621 atcccttttt actaaagtgc tccaatacaa ggggtaacgt attactcatt ttcggccatg
     7681 ctttttcact atcgtggaat accacaatgg aaccattgtt cgtatgcctg gcaacattct
     7741 caaaacaatt ttctgcactg accgatttgt cgaagtcgcc gctcagcaca tcccacatta
     7801 taatacggta atgatgcatt tttttgacct gggccgacct gatctttcca taaggcggcc
     7861 ggaaaagcgg ggattgaata taccgtttgg ccagacctac attccgtaaa tatgtctccg
     7921 ttttggtgaa ccagccattc aggtgatcct gtgtatgatt acctaccgaa tgaccttctg
     7981 ctactatccg ctggaaaatt tcagggtaag ctgccacatt ttttcccagg cagaaaaaag
     8041 tagcttttgc ctcatacttt ttcaaggtat cgagcacaaa cggggtggct atgggatgcg
     8101 ggccatcatc aaaactcagg taaatttttt tcgcagaagt atccatctgc catatccttc
     8161 ttggataaag tcgtttcagc caccagggtg ttttaatcag atagtttcgc attgtctgaa
     8221 atagtccctg aattctttta cccggcaagg aaggccatct atctttgctg cctatgaata
     8281 aaagagtcag ggttcgtttt gctcccagtc ctaccggcgg cctgcacctc ggcggcgtca
     8341 gaactgtatt gtttaattat ttattcgccc gaaaacatgg tggcgatttc ctggtgcgga
     8401 tcgaagatac cgaccagaac cgttatgtgc ccggcgcgga agaatatatt tttgacaccc
     8461 tgaaatggtg tggtctggag cctgatgaaa gtgtgcagca tgggggtaat tatggcccct
     8521 atcgtcagag tgaacgaaaa gccagttacc ggatattcgc tgaacaattg gtaaatgccg
     8581 gtcatgcgta ttatgcattt gataccccgg aagaactgga aaccatgcgg caggaatata
     8641 aaacgccgga aaatccttcc ccgcaatatg accacagcat ccggatgaaa atgcggaatt
     8701 ccctcagctt aaccgctgag gaaactgcgc aattactggc caacggcacc cgccatgtga
     8761 tccgtattaa aatgccggaa aatgaaaccg tcagtttcac cgatatgatc cgtggcgaag
     8821 tgagttttga gtctggtacc gtagatgata aagtattatt gaaagccgat ggcatgccta
     8881 cctatcacct tgcggtggtg gtggatgatc aggccatgga aatatctcac gctttccgcg
     8941 gggaagaatg gctgccctcg gccccggtgc atatactatt atggaaatat cttttcggtc
     9001 tggaccagat gccccaatgg gcgcactttc ccctgatcct cggaccgagt ggtaagctta
     9061 gcaaaagaga tggggctaaa tatggtttcc ccgtttttgc catgaactgg actgatccaa
     9121 aaacaggtga gcttaccgag ggctttaaag aaaaaggatt cttacccgaa gcctttatca
     9181 acctgctggc cctgcttggc tggaatgaag gcaccgaaca ggagatctat accaaagaag
     9241 agctgatcca aaa
//
LOCUS       DAOPMW010000099         9236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8190_length_9236_cov_4.780852, whole genome shotgun sequence.
ACCESSION   DAOPMW010000099 DAOPMW010000000
VERSION     DAOPMW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8190_length_9236_cov_4.780852"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2800
                     /locus_tag="PLU11_06570"
     CDS             <1..2800
                     /locus_tag="PLU11_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN58816.1"
                     /translation="PIYTNITYPFPKNPPFVGEDNPVGTYRREFTLPAVWDDKEIILH
                     FGSITGCAFIYVNGVKVGMSKVSKTAAEFNITSLVRPGKNLLAVQVIRWHDGSYLEDQ
                     DFWRISGIERDVFLFAMPKLTIWDYFIKAGLDEKYTNGVFSADVNLRAFKSSVQQKGT
                     IEITIADTDGKEVFRQLKKFGRSADSMQSIRFAGTVKNPRKWSAESPALYQCMIELKD
                     ETGKTVALTGSRIGFRTVEIKNAQLLVNGVRVMVHGTNRHEHDDVLGHVPTLALMKKD
                     IQLMKQFNINSVRTSHYPNDPLWYKLCDEYGLYLVDEANIESHGMGAEKQARFNPGAH
                     VAYRSEWAPAHLDRHRRMVERDKNHTSVIIWSLGNECGNGPVFHEGYKWIKERDNTRP
                     VQFEQAAENANTDIVCPMYPRIHDMKSYADDKSKQRPYIMCEFSHAMGNSNGNFQKYF
                     DIIESSPHMQGGFIWDWVDQGFKQTDKSGTVYWGYGGDLGSYNYLNDENFCANGLIAA
                     NRTPHPGLYEVKKVYQDIRFVAKDISKGQLMVKNNFAFNSLEKYTFYWTLVRNGQEVH
                     RETIAVSAAAGKQQEVTLPVPEDKTTQPGEYFIHVYAVISKSAATEMLPEGHEIAREE
                     FKLSGDYFSTTKATAGKLSLIHATDKISFQSEAVSGEIDTRTGALRNYRTSGGTQLSR
                     FPEPYFWRAPTDNDFGNSMPSNLGIWRSAHQSRKVKTVKVSEPGPEGLAVKVEYELTG
                     IAVPYTVEYLVMNDGAIRVTASIDLTGRDLPEMPRFGMRTQLAPEYNQLAYYGRGPWE
                     NYTDRKTASFLGQYSGLVKDQFTANYIRPQENGYKTDVRWLTLTSSSGQGIRIEGIQP
                     LCFSALNYSTESLDPGMTKKQQHPSDLRPDNNVYLNIDLLQRGVGGDDSWGALPHEEY
                     RLREKKYTYSYIIRLLDK"
     gene            2869..3660
                     /locus_tag="PLU11_06575"
     CDS             2869..3660
                     /locus_tag="PLU11_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPN58817.1"
                     /translation="MTAASSISLADTSGYRLVWSDEFNYNGKPDTTSWNYEYGFVRNN
                     ELQWYQPENAFCEHGLLVIEARKEHKPNPRFDSLNDNWRKSREHIEYTSSCLLTRGKQ
                     SWLYGRFEMRARIGISPGMWPAWWTLGIKQPWPANGEIDIMEYYRGKLLANIACLGVD
                     KKAHWFSNTLSTDSLGAEAWAARFHVWRMDWTSEFIALFCDDQLLNKVSLDSLVNRDG
                     TGLNPFRQPHYMLINLAIGGDNGGDPSGTKFPQRLEVDYVRVYEW"
     gene            3805..5811
                     /locus_tag="PLU11_06580"
     CDS             3805..5811
                     /locus_tag="PLU11_06580"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HPN58818.1"
                     /translation="MSAQITVALPPDYIQTVNGSTVFKVDQPDYVKSPFTGVTRKHWK
                     EAALYLLEGAFSYVKRMEDPMQFPKQPGKSYPRTVSQIPTEKLEGLCRTLFMAAPLLK
                     EHPGLVINNIRVADYYRYHILKLLDPSSPTYIKPQAKGGGPNQILVEFGALAVSMFYI
                     PEILFDPLSQEQKDILAATMISYGDGKTVPSNWKFFNIFVLSFFKAHGYAVNEQLLKE
                     YLDKSLEHYRGNGWYNDNPAYDYYSMWAFQMYGPVWAEYFGKKYYPEYAAKFMKNFSE
                     VKDNYPYLFGRDGKMVMWGRSMTYRIGSVAPLSLMGLENDPATNYGWMRRISSGVLLQ
                     FLQHPDFMKDNVPTLGFYGAFEPAVQSYSCRGSVYWMGKAFVSLLLPETNPFWSAVEN
                     EGPWGKDMNKGQVYNKFQDSSHILITDYPNSGAAEIRAWCNVKAIGANEPFRASENYN
                     RLSYNSAFPWQSDSAEGVVAMNYIFRNKKNEWEPLRLYAFKKFENGIYYREAAVETQP
                     GIRLQLADIPLPDGILRVDRISADTVTEMRLGHYALPNLSGTMRKEQRMVKGHKVQII
                     DNGVYQLAMVMINAWEGIYTADARGIHPEAVESSVINVRAITKANITSPTLYITVLLW
                     KKSGERWTDKELIPVKKYAYDRKTGSLKVQMRHLTGMPRVYHVF"
     gene            complement(5842..7998)
                     /locus_tag="PLU11_06585"
     CDS             complement(5842..7998)
                     /locus_tag="PLU11_06585"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HPN58819.1"
                     /translation="MYKYLLAPVILLTSFIATASIIPVKNNAELQEADKRALPGDTIV
                     LKNGIWKDVLISLNCQGTEDKPVIFRAETAGKVLVTGKSRLEIGGQYIVAEGLFFTNG
                     YSGKDPVISFRSGKERVSNNCRVTNCVIDDFNNPGRMEENYWIAFYGKNNRLDHCSFR
                     HKLNIGVLLAVILDDERSRENHHSIDHNYFGRRIPLASNGGEMIRVGVSQHCEFNSNT
                     KITDNFFEQCDGETEIVSIKSGSNEVTNNVFRECQGSVVLRHGNYNTVSGNYFLGNNK
                     SGSGGVRVINKGQQVLNNIFYQCRGTDFRSPLAVMNGIPNSPAHRYVQVTDAVISGNT
                     FYECTPATFCEGSDAERTLPPDKVTVTNNNFYNTKDSVIYKSYDRMDGILFSNNLVNR
                     GARQILPAGFTKGNDAPAKKAISMISQVEKKTRLNTGAGWYNKNNNLPAISWKQVNCP
                     DLLSLTKALASPEPVAIRLTGNAYKTDQPLLITKRVQISSHGTGFTLLSSSPIESLFI
                     IEGKGELQLNKLRLDIAGLQVSSFISSSKAAPSAHYNLLLTNSQIKNLGKQFPAVSLI
                     FANKSMVADSLIIRNCTFSNFNAGLLHMTEEKDNKGYYNAEKIVISNNSINNLQGALL
                     AIYRGGNDESTMGPDLTFSNNRINNSNNGSTTPLIHLYGVQKSRMEKNQFINCNTGNI
                     LITYEDVVRAMHQLKNNQFINSGSVQKNEFVREENK"
     gene            complement(8002..9042)
                     /locus_tag="PLU11_06590"
     CDS             complement(8002..9042)
                     /locus_tag="PLU11_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN58820.1"
                     /translation="MNKPATIKDIAKQLNISISTVSRAIRNAADVNPETKRAVMALAE
                     ELDYQPNRLALSLKQKQTHNIGVLVPNLDYVLSTMVKGIDEVALEAGYTVMVCQSNES
                     FGREIVNTRRLLDSLVDGFIISVSSETKVFDHFKKILDKKMPVVVFDRVTPFLQAPGV
                     RIDNEDGGFQATEHLIEQGYKRIAILAGPKNLQISNQRMEGYLKSLRKHKIKPDKELI
                     IYCDFNQDYAYLATKELLAMKKRPDAIFTISDRMAIGAMLAIKEKGLKMPHDIGLVGF
                     NNEPVTSLVTPGISSVDQPAFELGKIAAKLFIEKMHRDTDMSQVEEVLKPKLIIRESS
                     LRILPRGGRTKQ"
BASE COUNT         2449 a         1952 c         2285 g         2550 t
ORIGIN      
        1 tccgatctat accaatatca cttacccgtt ccccaaaaat ccgccctttg tgggggaaga
       61 taatccggtc ggcacctatc gccgggaatt tacactgcct gcagtatggg acgataaaga
      121 gattatactt cattttgggt cgataacggg atgtgcattc atctatgtga acggggtgaa
      181 agtgggtatg agtaaagtgt ccaagaccgc ggcagaattt aatatcactt ctttagtccg
      241 gccggggaaa aatctccttg ccgtgcaggt aatccgctgg catgatggca gctacctgga
      301 agaccaggac ttctggcgga tcagtgggat agaacgggat gtgtttctgt ttgccatgcc
      361 gaaactgacg atctgggact actttattaa ggccggacta gacgagaaat atacaaatgg
      421 tgtttttagc gctgatgtga atctgcgggc ctttaaatcg tccgttcagc aaaagggcac
      481 tattgaaatc acgattgctg atactgatgg caaagaggtt ttcaggcagc taaagaaatt
      541 tggcaggagc gcggatagta tgcaatccat ccgttttgcc ggcacggtaa aaaatccccg
      601 gaaatggagt gcggaatcac ccgcacttta tcaatgtatg attgaattaa aggatgagac
      661 gggtaaaacc gttgcactga ctggcagccg gatcggtttc cggactgtgg aaattaaaaa
      721 tgctcaattg ctggtgaatg gtgtacgggt gatggttcat ggcaccaacc gccatgagca
      781 tgatgatgtg ctcgggcatg tgcctacact tgccttgatg aagaaggata ttcagctgat
      841 gaagcagttt aatattaatt ccgtacgtac ttcccattat cccaatgatc cgctttggta
      901 taaactctgc gatgagtacg gattatacct ggtggatgaa gccaatattg aaagtcatgg
      961 gatgggtgcg gaaaaacagg cccgctttaa tccgggtgcc cacgtggcgt atcgttcgga
     1021 atgggcgcca gctcatctgg accgccaccg gaggatggtg gaacgggata agaatcacac
     1081 ttcagtgatc atctggtcat tggggaacga atgtgggaat ggtccggtat ttcatgaagg
     1141 atataaatgg atcaaggaac gggacaatac acgccctgta caatttgaac aggcagctga
     1201 aaatgcgaac accgatatcg tttgcccgat gtatccgcgt atccatgaca tgaaatccta
     1261 tgcggatgac aagtcaaagc aacgtcctta tatcatgtgc gaattttcac atgcgatggg
     1321 gaacagcaac ggtaacttcc aaaaatactt tgatataata gaatccagcc cgcatatgca
     1381 gggtggtttt atctgggatt gggtggacca gggatttaaa caaacggata aaagcggaac
     1441 ggtttactgg ggttatggcg gcgacctggg tagctataat tacctgaatg atgaaaactt
     1501 ttgtgccaat ggtttgattg cggccaaccg gacaccgcat ccgggtttat atgaagttaa
     1561 aaaagtatac caggatatcc gttttgtggc aaaagatatc agtaaaggac agttgatggt
     1621 gaaaaataat tttgccttta attcattgga aaaatatact ttctactgga cattagtacg
     1681 gaacgggcag gaagtacacc gtgaaacaat tgcagtcagt gcagctgccg gtaaacaaca
     1741 ggaggttacc cttcctgtcc ctgaggataa aacaacgcag cccggtgagt atttcattca
     1801 tgtatacgcg gtaatcagta agtccgccgc gactgaaatg ttgcctgaag gtcatgagat
     1861 agcccgggaa gaatttaaac tcagtgggga ttatttcagt accaccaagg ctactgccgg
     1921 caaactgtcg ctgatccacg caaccgataa aatcagtttt cagtccgaag ctgtttcggg
     1981 tgaaattgat acccgtacag gtgctttacg taattataga accagtggcg gaacacagct
     2041 gtcccggttc ccggaacctt atttctggag agcccccaca gataacgact ttggaaacag
     2101 tatgccatct aatctgggta tctggcgttc cgctcaccag tcccggaaag taaaaacagt
     2161 gaaggtcagc gaacctgggc cggaaggttt ggctgttaaa gtggagtatg aacttacggg
     2221 gatagccgta ccttatacgg ttgagtacct cgtgatgaat gatggtgcga tccgtgttac
     2281 agcttccatc gatcttacag gaagggattt gccggaaatg ccccgttttg gtatgcgtac
     2341 acagttagca ccggaatata atcaactggc ctactatggc cggggtccat gggagaatta
     2401 taccgatcgt aaaacggctt ctttcctcgg acaatacagc ggactggtaa aggatcagtt
     2461 tacggcaaat tatattcgcc cgcaggaaaa cgggtataaa accgatgtcc gctggctgac
     2521 gctgacatca tcatcgggac agggaatccg gatagaaggc atacagccat tatgtttcag
     2581 tgcattgaat tactccacag agagccttga tccgggtatg accaaaaagc aacagcatcc
     2641 atccgatctg cgtccggata acaatgtata cctgaatatt gatctattac aacgaggagt
     2701 agggggggat gacagctggg gggcactgcc gcatgaggag taccgtttgc gtgagaaaaa
     2761 atatacgtac agttatatca tccgtttgct ggataaataa ttaacctgtt gaaaagcctg
     2821 atttcttttt ttcagatacc gctgttcctg attgtccttt gtcaaacagt gacagcagca
     2881 tcatccattt ctctggctga taccagcggc taccgattgg tttggtccga tgaattcaat
     2941 tataatggta agcctgatac tacaagctgg aattatgagt atggatttgt ccgcaataat
     3001 gaattgcagt ggtaccagcc ggagaatgca ttttgtgagc atggactatt ggtgattgaa
     3061 gccagaaaag agcataagcc caatccccgg tttgatagtc tcaatgataa ctggagaaaa
     3121 agcagggaac atattgaata tacatcttcc tgcctgctga cacgagggaa acagagctgg
     3181 ttgtatggcc ggtttgaaat gcgggccagg atcgggatca gtccgggtat gtggcccgcc
     3241 tggtggactt tgggcataaa gcaaccctgg ccggccaacg gggagatcga tatcatggaa
     3301 tattaccggg gtaagttact ggccaatatc gcctgtcttg gtgtcgataa aaaagcacat
     3361 tggttcagca atacactttc cactgattca ttgggagccg aagcatgggc cgcccggttt
     3421 catgtgtggc gtatggactg gaccagtgaa tttattgctc ttttttgcga tgatcaattg
     3481 ctgaataaag tgtcactgga ttcactggtc aacagggatg gtaccggatt gaatccattc
     3541 cgtcagccac attatatgct gatcaacctg gcgattgggg gcgataatgg cggagatccg
     3601 tcgggtacaa aatttccgca gcggctggaa gtggattatg tacgggtgta tgagtggtaa
     3661 gttttaattt ataagttgta agtaataagt tggaagtgat aagttgtaag taatacgtgg
     3721 taaggtttaa tatagtttac tatgggtaga aagataatcg ggaagttatg gatagccttt
     3781 ttttttgtgt cgtctttaaa accgatgtca gcgcaaatta ccgttgcgtt accacctgac
     3841 tatattcaaa cagtaaatgg aagcacggtt tttaaggtgg atcagccgga ttatgtcaaa
     3901 agtccattta ccggggtgac ccgtaaacac tggaaggagg ccgctttata cttattagaa
     3961 ggagcattca gttatgtgaa gcgaatggaa gacccgatgc agtttccgaa gcaacctgga
     4021 aaatcttatc ccagaacggt cagccagata ccaacagaga aattggaggg gctctgccgg
     4081 acactcttta tggccgctcc attactgaag gaacacccgg gcctggtgat taataatatc
     4141 agggtcgccg attattaccg gtatcatata ttgaaactac tggatccttc cagccccact
     4201 tatataaaac cacaggcaaa aggaggaggt ccgaatcaga tcctcgtgga attcggggca
     4261 ctggcagtat ctatgtttta tataccggaa atcctgtttg atccgctgtc acaggaacaa
     4321 aaagatattc tcgcggcaac tatgatcagt tatggtgatg gtaagacagt accttccaac
     4381 tggaagttct tcaacatttt cgtactgagt ttttttaaag cacatgggta tgcggtcaat
     4441 gagcaattat taaaagagta cctggataaa tctctggagc attaccgggg aaatggctgg
     4501 tacaatgaca atccggccta tgattattat agtatgtggg cttttcaaat gtatggacct
     4561 gtatgggcgg aatattttgg gaaaaagtat tacccggagt atgcagcaaa attcatgaaa
     4621 aatttcagcg aagtaaagga caattatcct tacctctttg gacgtgatgg taagatggtg
     4681 atgtggggcc ggagtatgac ttaccggatc ggttcggtgg ctccattgtc attgatggga
     4741 ctggagaacg atcccgccac caattatggt tggatgcgcc ggatttcttc cggagtctta
     4801 cttcagtttc tgcagcatcc tgattttatg aaagacaatg tgcctaccct tggtttctat
     4861 ggggcttttg aacccgccgt tcaatcttac agttgccggg gaagtgtgta ctggatgggg
     4921 aaagcctttg tctccctgtt gttgccagag accaatccgt tttggtccgc tgttgaaaat
     4981 gaaggtccct ggggaaagga catgaataaa ggacaggtgt acaacaaatt ccaggatagt
     5041 tcgcatatcc tgatcaccga ctatcccaat agtggcgctg ctgaaatccg ggcctggtgt
     5101 aatgtcaaag ccatcggggc caatgaaccc ttccgggcca gtgagaatta taaccggcta
     5161 tcttacaaca gcgctttccc ctggcagtcc gacagtgcag aaggagtggt ggcgatgaat
     5221 tatattttcc ggaataagaa aaatgaatgg gaacctttac gtctctatgc atttaaaaag
     5281 tttgaaaatg gaatatatta ccgggaggct gcagtggaaa cacaacccgg tatccgttta
     5341 caactggcag acatcccttt gccggatgga atactccggg tcgaccggat cagtgcagat
     5401 acggtaaccg agatgcggct gggacattat gctttaccca acctttctgg tacgatgcgg
     5461 aaagagcagc ggatggtaaa aggacataag gtgcaaatca ttgacaatgg agtgtatcag
     5521 ctggccatgg tcatgataaa tgcctgggag ggtatctata ccgccgatgc caggggtatt
     5581 catccggaag ccgttgaaag cagcgtaatt aatgtacggg ccattacaaa agccaatata
     5641 actagtccaa cattgtatat cactgtattg ctctggaaga aatccggtga acgatggacg
     5701 gataaggaac tgattccggt aaagaaatat gcgtatgacc gtaaaaccgg ctcgttaaaa
     5761 gtgcagatgc gtcatttaac cgggatgccc cgggtgtacc atgttttttg atttattgta
     5821 agacgcttat atcactcggg gttatttatt ttcctcccgg acaaattcat ttttctgcac
     5881 actgccggaa ttgatgaatt ggttgttttt taactgatgc atggccctga ccacatcttc
     5941 atacgtaatc aggatattgc cggtgttgca attaatgaac tggtttttct ccatccggga
     6001 tttttgcacg ccatacagat ggatcagggg cgttgttgag ccgttgttgc tgttgtttat
     6061 ccggttatta ctgaatgtaa gatccggccc catcgtgctc tcatcattgc caccacggta
     6121 aatggccagt aatgcaccct gcaggttatt aatgctgttg ttgctgatca cgattttctc
     6181 cgcgttgtaa tatcctttat tgtctttttc ttcggtcata tgaagcaatc ctgcattaaa
     6241 attgctaaaa gtacaattgc ggatgatgag gctgtcggcc accatgcttt tattggcaaa
     6301 aatgaggctg acggccggga attgctttcc caggtttttg atctggctat tggtaagcag
     6361 cagattataa tgggctgacg gagcagcttt gctgctggaa ataaaagatg aaacctgcaa
     6421 gcccgctatg tccagccgga gtttatttaa ttgcaattct ccttttcctt caatgataaa
     6481 caggctttca atcggggagg agctgagcag ggtaaagccg gtgccatggc tgctgatctg
     6541 aacccgtttg gtgattaata agggttgatc tgttttgtag gcattaccgg tcagccgtat
     6601 ggctaccggc tcaggagaag ccaatgcctt tgtcaggctc agcaggtccg ggcagtttac
     6661 ttgtttccag ctaatggcgg gcaggttgtt gtttttgtta taccagccgg caccagtgtt
     6721 cagccgggtt ttcttttcca cctggctgat catactgatt gcttttttgg caggggcatc
     6781 gtttcctttt gtaaatccag ctggtagtat ttgacgggcg ccacgattga ccaggttgtt
     6841 gctgaataat atgccatcca tccggtcata acttttatag atcacgctgt ctttggtatt
     6901 atagaaatta ttattggtga ccgttacttt atccggcggc aatgtccgtt ctgcatcact
     6961 gccttcgcag aaagtggccg gggtacattc gtaaaatgta tttccgctga ttacggcgtc
     7021 ggttacctgc acataccggt gtgccggaga attggggatg ccgttcatca cggccagtgg
     7081 tgaacggaaa tcggtgccac ggcattggta aaagatattg ttcagtacct gttgtccttt
     7141 gttgattaca cgtacgccac cgctgccgga tttgttattg cccaggaagt aattgccgga
     7201 aacagtattg tagtttccat gacgtaatac gactgagccc tggcattcgc ggaatacatt
     7261 attggttact tcattgctgc cggatttgat agaaacgatt tccgtttccc catcacattg
     7321 ttcaaagaaa ttatcagtga tttttgtgtt tgaattgaat tcacaatgct gggaaacgcc
     7381 cacacggatc atttccccgc cattggaagc cagcggtatc cggcgtccga agtaattatg
     7441 gtcgatggaa tggtggtttt ccctgctgcg ttcatcatca aggataacgg ccagtaatac
     7501 accgatattg agtttatgcc gaaaactgca atgatccaac cggttatttt taccatagaa
     7561 tgctatccag taattctctt ccatccgtcc gggattattg aagtcgtcaa tcacacaatt
     7621 cgtcacccgg caattattgg atacccgttc tttgccactc cggaagctga tcacgggatc
     7681 tttgccggaa tagccattgg taaagaataa accttctgcc acaatatatt ggcctccgat
     7741 ttcgagacgt gattttcctg taaccaatac tttccccgct gtctccgccc ggaaaataac
     7801 cggtttgtct tcggttccct ggcagttcag gctaatcagg acatccttcc atattccgtt
     7861 tttcaaaaca atcgtatcgc ctggcagtgc tcttttatca gcctcctgca gttctgcatt
     7921 gtttttcacc ggtatgatgg aagcggtggc aatgaaagaa gttaaaagaa tgaccggcgc
     7981 taaaagatat ttgtacatgt tttattgttt tgtccgaccg cccctgggga gtatacggag
     8041 ggaagattca cggatgatca gtttgggttt taatacttcc tctacctggc tcatgtcagt
     8101 atcgcgatgc attttttcta taaaaagttt cgcggcaatt tttcccaatt caaaggccgg
     8161 ttggtccaca ctggaaatac ccggggtcac gagactggtc accggttcgt tattaaaacc
     8221 gactaagccg atatcatgtg gcatttttag ccctttttct ttgattgcga gcatggcacc
     8281 aatggccatc cggtcactga tggtgaaaat agcatcgggc ctttttttca ttgccagcag
     8341 ttccttagtg gcgagatagg catagtcctg gttgaaatca cagtagatga tcagctcctt
     8401 atcaggtttg atcttgtgtt ttctcagact tttcaggtat ccttccatcc gttggttact
     8461 gatctgcagg ttcttcggac cggccagaat agcaattcgt ttatacccct gttcaattaa
     8521 atgttccgta gcctggaaac caccatcttc attgtcaata cgcacccccg gtgcctgcag
     8581 gaaaggagtc acccggtcaa ataccaccac gggcattttt ttgtccagta tttttttgaa
     8641 gtgatcaaag actttggttt cactggaaac ggaaataata aatccatcaa caagactatc
     8701 cagcaaacga cgggtattta caatttcccg gccaaaggat tcattactct gacagaccat
     8761 caccgtgtag ccggcttcca gcgccacttc atcaatccct ttaaccatgg tggaaagcac
     8821 ataatcgagg ttaggtacca ataccccgat attgtgggtt tgtttttgtt tcagactcag
     8881 ggcaagccgg ttaggctgat aatccagttc ctccgccaat gccatcaccg ctctcttggt
     8941 ttccgggttt acatctgcgg cgttgcggat ggccctggat acggtagaga tggaaatgtt
     9001 aagttgcttg gcaatgtctt taatcgtggc aggcttattc atatcgttcc gggatcaggg
     9061 attaaagcta ggaagaagaa gtttacgaaa atcccggcag cgttgccggt aacgttctcg
     9121 gtgcccttta tttagaaaat atgtaaaaaa taggataaat atcttattta aattaaaaaa
     9181 agtactttta attaaaaata ttctaataca caaaaaaaag cccgccagat tattcc
//
LOCUS       DAOPMW010000100         9171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8285_length_9171_cov_8.609149, whole genome shotgun sequence.
ACCESSION   DAOPMW010000100 DAOPMW010000000
VERSION     DAOPMW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_9171_cov_8.609149"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLU11_06595"
     CDS             complement(<1..537)
                     /locus_tag="PLU11_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPN58821.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELCQELVLLDIKEGVAEGKAQD
                     MMQTATLLGFDTKISGSTNDYSKTAGSDVVVITSGLPRKPGMTREELIGVNAGIVKGV
                     CENILKHSPNAIIIVISNPMDTMTYLALQSTGLPKNRIIGMGGTLDSSRFKYQLSQHL
                     GCSPADLNAVVVGGHGDTT"
     gene            complement(643..1143)
                     /locus_tag="PLU11_06600"
     CDS             complement(643..1143)
                     /locus_tag="PLU11_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPN58822.1"
                     /translation="MKKMLLILVLALLALPSFSQKDSTLAPYKRFPGFPPISLLLPDG
                     KSYFTKADLPKDKAVLLMLFNPSCSHCQEETKQITQHIDKFSNIHIVMATPMPLDSML
                     AFREKYKLANFKNITITQDNKTMMPTYFMINNLPFLAFYNRKKELIGTHEGSMTIEKL
                     LATFKN"
     gene            complement(1175..3958)
                     /locus_tag="PLU11_06605"
     CDS             complement(1175..3958)
                     /locus_tag="PLU11_06605"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPN58823.1"
                     /translation="MLTTIGVDAQLIITPHPNAQSLAQRLVGEGVTISNVSFTGNPLM
                     ASFFLNRANRTNIGIDSGIVLTTGQAKSNGADWGTDGNGTGLASTLEASGNWGLPGDN
                     DLSTAVGNFNLEDACVLEFDFVPLGDSIRFNYVFSSEEYVPDYVCSFNDVFAFFISGP
                     GITGLKNIALIPNTNTPVSIFNVNNVPGGACPNNPQYYIDNSSNVFFTHDGHTTVLTA
                     RERVQPCQTYHLKLVLADSGDDMVDTGVFLQAKSLSSNTFGLTNLTQTDATGNSYLVE
                     GCATGAFNITRPRRDPFPLSISLSYGGQATNGIDMQAMPLNVVIPANDSFVTVNVIPL
                     MDGMAEGIETIKIYALAGCAAGTPTDSTFIQIRDYDILNLTPDTARVCQGNSVQLIAS
                     PGYTVYQWNADPTLSSTSIPAPLATPVNPSTTYICTATVGTCQAKDSVYVEVKKASFL
                     SKTDVNCRAGVTGSLQVSAGVGWLSPVQYSLDGINWQSSNSFTNLAVGNYMVKVRDAD
                     CTDSVAITISQAFPDLTISSPAITAATCSGNPDGQFTATAQGGKAPYTWSADGISFQS
                     SPLFNLNQGNYTITVKDENGCLQTSPATIPLINTVTIDAGADPFICEGKSFQLNASSN
                     ATQFAWTPAISLSNAGILNPTANPSTTTKYYVTATTGICSLTDSITINVWPAPVPDAG
                     TDFSDCFGKIVRLNGSGGVAYQWSPTTYFTGPSNVSNPALKATASTSYYLSVTDARGC
                     QSLQDDEVRLTVTPAVRINAGRDTVAAMNQPVQLQASELSSAGVIRYNWTSPQFLDNP
                     GIANPVATLPYDFRYTVTGTTADGCEGLDEILIKVYKGPEIYVPTGFSPDGNGLNDVL
                     RAFPVGIKEFRYFRVFNRWGQLVFQTKNPGQGWDGTINGRPQPIGSFIWMAEAVDYQG
                     KLLFRKGTVTILR"
     gene            4116..4682
                     /gene="efp"
                     /locus_tag="PLU11_06610"
     CDS             4116..4682
                     /gene="efp"
                     /locus_tag="PLU11_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPN58824.1"
                     /translation="MASTADISRGLIIKLDGSLYTVVEFGENKTARAAAKVWAKLKGV
                     DNNRSIEKTWNSGDNIFPVRVERKSYQFLYKDDTGYNFMDTETFEQVAIQESLVDAPQ
                     FLKDGQEVSVLFNTETELPMSVELPDKIVLLVTYTEPGLRGDTATRTLKPATVETGGT
                     VNVPLFVNEGELIRVNTKTGEYVERVKE"
     gene            4944..5426
                     /gene="accB"
                     /locus_tag="PLU11_06615"
     CDS             4944..5426
                     /gene="accB"
                     /locus_tag="PLU11_06615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPN58825.1"
                     /translation="MDFKQIQELIKMVNKSNIGELTIEQKDFRVTIKQKEEHVTQVMS
                     APVQAAPVAIAAPQPVAAAAASSSADKPKAAEPVATNLITIKSPMIGTFYRKASPDKP
                     NFVEVGSDVTSGSVVCIIEAMKLFNEIESEVSGKIVKVLVDDASPVEYDQPLFLVEPA
                     "
     gene            5545..6882
                     /gene="accC"
                     /locus_tag="PLU11_06620"
     CDS             5545..6882
                     /gene="accC"
                     /locus_tag="PLU11_06620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPN58826.1"
                     /translation="MFKKILIANRGEIALRVIRTAREMGIKTVAVYSTADRDSLHVKF
                     ADEAVCIGRPQSGDSYLNIPHLMAAAEITNADAIHPGYGFLAENAKFAQICNDHGIKF
                     IGPTPDMINAMGDKITAKETMIKAGVPVVPGGEGLLQSVDEAKGLAKNMGYPVILKAT
                     AGGGGKGMRIVWEESEIEKAYDTAKAEAAAAFKNDGIYMEKFVEEPRHIEIQVAGDQY
                     GTVCHLSERDCSIQRRHQKLVEESPSPFMTDELRYKMGEAAKKAAGAINYESVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHCVTEEVINFDLIKEQILIAAGHRISGKDYIPQMHSIE
                     CRINAEDPYNDFRPSPGKITVLHQPGGHGVRVDSHAYAGYVIPPYYDSMIGKLITVAQ
                     TRQEAIQTMYRALSEYVIEGVKTTIPFHLQLMQDERFRSGDFNTKFLEGFTLR"
     gene            complement(7031..7729)
                     /locus_tag="PLU11_06625"
     CDS             complement(7031..7729)
                     /locus_tag="PLU11_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HPN58827.1"
                     /translation="MDRSGFFKQAFSLLKKAAVPPKEVPEEKPAALPAEVTDPSALTS
                     IEIGYCNKAGLHPDTGIFLKKLTKRPIEKLVFEDYDTETNKPSGICSFCPEEEARAII
                     CQYQQEFISKGQFLYISDIPGNGLQVAILNGITDPYLIMQLTETNGANHELMTADIIE
                     QLKKWDASFGVRITGIGFDFCECAIVNKEIDYNKLAAEIYQFCPDVVDQGTETLEALE
                     DELRRTGSIFLWWD"
     gene            complement(7797..8468)
                     /locus_tag="PLU11_06630"
     CDS             complement(7797..8468)
                     /locus_tag="PLU11_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN58828.1"
                     /translation="MNRIILIVLLFAGLTVSAQNQVALFAGPQMSTANYSIRNQKQET
                     GFKYGLHAGIGMKVPFDGFLYFAPSVYYSYKGYDVTYNLPNSLPDIDAIDNSTRFHTM
                     ELAALLQFDLSPKPSHVFIKAGPALDFMLFAKEKFNLSGGGSVSRNMKFGPGEYGRYS
                     ANLISQLGYEKDGGFIVYFQFTHSPANLSNRDGGPSIKHKVFGLTFGKYLNRKKIIID
                     TRVRE"
     gene            8641..8883
                     /locus_tag="PLU11_06635"
     CDS             8641..8883
                     /locus_tag="PLU11_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58829.1"
                     /translation="MDPDVFIGNFVVFLFVDFNTKYIFPQTGADNHADGADLISIRCG
                     FPQFEPVIFTGYSVLFPMVVGRAGLNFGGIKIMSHA"
     gene            8876..>9171
                     /locus_tag="PLU11_06640"
     CDS             8876..>9171
                     /locus_tag="PLU11_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPN58830.1"
                     /translation="MHENDISFLIRKAIFTVYNTLGPGLYESVYITALRHELEKLGLM
                     VSVEKPLPLIYDGIRFEAGFRIDMMVNERVIIEVKSVEKLAEVHHKQIITYLRLS"
BASE COUNT         2413 a         2021 c         2309 g         2428 t
ORIGIN      
        1 ggtcgtatca ccgtgaccac ctaccactac ggcattcagg tcagcaggac tgcagcccag
       61 gtgctgactt aattgatatt tgaaacggct gctgtcgagt gtaccaccca taccgatgat
      121 cctgtttttg ggaagtccgg tagactgcag ggccaggtag gtcatggtat ccatcgggtt
      181 actgatcacg ataatgatcg cattgggaga atgtttcagg atgttttcac aaactccttt
      241 cacgataccg gcatttacac cgatcagttc ttcccgggtc attccgggtt tacggggaag
      301 accagacgta atcactacca catcactgcc ggctgttttt gaatagtcat tggtactgcc
      361 gctgatcttg gtatcaaaac caagcaaggt ggcagtctgc atcatatcct gtgccttacc
      421 ttcggcaact ccttctttaa tatccagtaa tacaagttcc tggcaaagct ctttacgggc
      481 aatattatca gcacaggttg cacctaccgc accagcgcct acaacagtta ctttcatgtg
      541 taaaaacgtt tatttgaatg aataattatt tacttgtctg ataccggctt ttgcagccgg
      601 cgcaaaggta gcggatgacc gttattttta acctgactgt tatcagtttt tgaaggtcgc
      661 cagcagtttt tcgattgtca tagacccttc atgggtgccg atcagttcct ttttccggtt
      721 ataaaaagcc aggaagggca ggttattgat cataaaataa gtaggcatca tggttttatt
      781 gtcctgggtt atcgtgatgt tcttaaaatt tgccagtttg tatttttccc ggaaagccag
      841 catggaatcc aggggcattg gcgtggccat cacaatatga atattgctga atttgtcaat
      901 gtgttgggtg atctgtttgg tttcttcctg gcaatgggaa caggaggggt tgaagagcat
      961 cagcaatacg gctttatcct tgggcagatc tgctttggta aaatagcttt ttccatcggg
     1021 cagcaagaga ctgatcgggg ggaatcccgg aaaccgttta tagggggcca gcgtgctgtc
     1081 tttttgagaa aaggagggca aggccagcag ggccagtacc agaatcagta acattttttt
     1141 catgcatcaa agataaaagg attcggtcaa cggattatcg caggattgtc acggttccct
     1201 tccggaacag gagcttaccc tggtagtcaa ccgcctcggc catccagata aaactcccga
     1261 taggctgggg ccggccattg atcgtgccat cccatccctg tcccgggttt tttgtctgga
     1321 agaccagctg cccccatcgg ttgaaaaccc tgaaataacg gaattctttt atacctaccg
     1381 ggaaggctct gagcacatcg ttcagcccgt taccgtccgg cgaaaaaccg gtcgggacat
     1441 aaatctccgg tcctttatac actttgatca gaatttcatc cagtccttca cagccatcag
     1501 ctgtagtccc cgttacggta taccggaaat cataaggaag ggtagcaacc gggttggcaa
     1561 tcccgggatt atccaggaat tgcggactgg tccagttata cctgatcacc ccggctgaac
     1621 tgagttcgga agcctgcaac tgaaccggct ggttcatggc tgcgactgta tcgcgtccgg
     1681 cattgatccg cacagctggc gtaaccgtca accgtacctc atcatcctgg agggattgac
     1741 agccacgggc atctgtaacc gacaagtaat aactggtact ggctgtggct tttaaggccg
     1801 gattggatac attggatgga ccggtaaaat aggtggtggg gctccattga taagccactc
     1861 cacccgatcc gtttagccgg acgattttgc cgaagcaatc cgagaaatca gttcctgcat
     1921 ccggcaccgg cgccggccaa acattgattg taattgaatc agtaaggctg catataccgg
     1981 tagtagccgt aacataatat ttggtggtcg tggacggatt ggcagtcgga ttcaggatgc
     2041 ctgcattgga aagtgaaatg gccggcgtcc atgcaaattg ggtggcattg gaactcgcgt
     2101 tgagctgaaa agatttgccc tcacagataa agggatccgc tcctgcatca atggttacgg
     2161 tattgatcag gggaatcgtt gccggactgg tttgcagaca cccgttttca tctttcaccg
     2221 ttatcgtata attcccctgg ttgagattga ataaaggaga agattgaaaa ctgatcccgt
     2281 cagctgacca ggtatagggt gcttttcctc cctgggcagt ggctgtgaac tggccatccg
     2341 gatttcctga acaggtagcc gccgtaattg cgggactgct gatggtcaga tccgggaaag
     2401 cctggctgat cgttatggcc acactgtccg tgcaatcggc atcgcggact ttcaccatat
     2461 agttaccaac ggccaggtta gtaaaactgt tactgctttg ccagttaatt ccatccagtg
     2521 agtactgaac cggggacaac cagccaacac cggcggatac ctggagactg ccggtgactc
     2581 cagcccggca attcacatct gtttttgaaa ggaagctggc ttttttcact tccacataaa
     2641 cggagtcctt tgcctggcag gtgcccactg tagcggtgca gatataggtg gttgaaggat
     2701 taaccggtgt ggcaagtggg gccgggatac ttgtactgct caaagttggg tcagcattcc
     2761 attgatacac tgtataacca ggtgaagcga ttaattgcac agagttgccc tggcaaaccc
     2821 tcgcggtatc cggggtgaga tttaatatat cataatcccg gatctgaatg aatgtgctgt
     2881 ccgtgggtgt accagctgca caaccggcga gtgcataaat tttgatggtt tcgatacctt
     2941 ctgccattcc gtccatcagc ggaattacat ttaccgttac aaaactatca ttggcaggta
     3001 tcactacatt cagtggcatc gcctgcatat caattccatt tgtagcctga ccaccatagg
     3061 agaggctgat actaagcggg aacgggtctc tgcgcggacg ggtgatatta aaggcaccgg
     3121 ttgcacaacc ttccacgagg taactgttcc ctgtagcatc ggtttgggtg agattcgtca
     3181 gtccgaatgt atttgaactc aggctttttg cctggaggaa aacaccggta tccaccatat
     3241 catctcctga atctgccagc accagtttca aatgataggt ctgacagggt tgtactcttt
     3301 cgcgggcagt cagtacggtg gtatgaccat catgtgtaaa gaatacattt gaactgttat
     3361 cgatataata ttgcggatta ttcgggcagg cacccccggg tacattattc acattaaaaa
     3421 tggaaactgg ggtgttggta ttgggaatca gggcaatatt tttaagaccg gtaattccgg
     3481 gacccgagat aaaaaaagcg aagacatcgt tgaatgaaca tacataatca gggacgtatt
     3541 cttctgaact gaatacataa ttaaaccgga tactgtcacc aaggggtaca aaatcaaact
     3601 ccagtacaca ggcatcttcc aggttgaaat ttccgactgc tgtactcagg tcattatcgc
     3661 cgggtaatcc ccagttaccg ctggcttcca atgttgaagc aagtccggtt ccattaccgt
     3721 ctgttcccca gtcagccccg tttgatttgg cctgaccggt ggttaataca atgccactgt
     3781 caatgccgat attagtgcgg ttggcgcgat ttaaaaaaaa tgaagccatc aggggattgc
     3841 cggtaaaact cacattgctg attgtgaccc cttcgcccac cagccgctgt gccagggact
     3901 gggcattcgg atgcggggta atgatcagtt gtgcatcaac cccgattgtg gttagcacca
     3961 gaaatactga taaaaagtaa tggtaaaatc cggatctcat aacagggtag cgaatatact
     4021 taaaaccgca gtagccggga attttagcct ggtcaagctt ccttttgctt tgcggtattc
     4081 ctcccttatc tttgccgcct ctaaaaagat aacaaatggc atcaacagca gacatcagca
     4141 ggggactcat catcaaactg gatgggagtt tatacactgt ggtagaattc ggagaaaaca
     4201 aaaccgccag ggccgccgcc aaagtgtggg ccaaactaaa aggggtggat aataaccgaa
     4261 gcattgaaaa gacctggaac tcaggcgata acatcttccc ggttcgggtt gaacggaaaa
     4321 gttatcagtt tttgtataaa gacgataccg gttataattt catggatact gaaacatttg
     4381 aacaggtggc tatccaggaa agtctggtcg atgcccctca gttcctgaaa gacggacagg
     4441 aagtaagcgt attgttcaat acagaaacgg aacttccgat gagtgtggag ctgcctgata
     4501 agatcgtgtt gctggtaacc tataccgagc ccggactaag aggtgatacc gcaacccgta
     4561 cactgaagcc agctacagtg gaaacaggtg gtactgtaaa cgtaccgctg tttgtgaatg
     4621 aaggcgagct gatccgggta aataccaaga cgggtgagta tgtagaaaga gtaaaagaat
     4681 agtttatcag ttttatatga aaatggccta aaatcgaccg gttttgggcc atttttgttt
     4741 gatttcgggt cattttgggt caaaatcagt aaaaattcat tgattttgtc cttttgattt
     4801 ccttacctta gcttcccttt tgggcgggaa agcaggcggg tatagttact gatcatcaaa
     4861 atcagtgagc cccgaatgac cagccggatt tgctgccgga aagcaaggct ttgtaaacca
     4921 aaacatctac aaaattgaaa aacatggact ttaagcaaat tcaggagttg atcaagatgg
     4981 tcaacaaatc gaatattggc gaattaacga ttgagcaaaa ggatttccgt gtcaccatca
     5041 agcagaagga agaacatgtg acccaggtga tgtcggcacc tgttcaggcg gccccagttg
     5101 ccattgccgc cccccaacca gtggccgccg ctgcagcctc atcctcggcc gataagccca
     5161 aggctgccga gccagttgcg accaatctga ttactattaa aagcccgatg attggtacat
     5221 tctaccgcaa agcctctccg gataaaccca attttgtgga agtaggttct gatgttacct
     5281 ccggatcggt ggtctgcatt atcgaagcca tgaaactgtt caatgaaatt gaaagcgaag
     5341 taagtggtaa gattgtaaaa gtgctggtgg acgatgcttc accagtggaa tatgatcagc
     5401 cgttgttcct ggtggaacca gcttaggaat tcaaaagtta aaattcaaaa gtcaaaaatc
     5461 ttctgttcga aaatgctgaa aggacagtag atgcttttgt tttttacttt ttacttttta
     5521 ctttttactt tttaattcta catcgtgttt aaaaaaatac ttatagcaaa ccgcggggag
     5581 attgccttac gtgtgatccg cacagcccgg gaaatgggga taaagaccgt tgcggtctac
     5641 tctaccgccg accgggacag tctgcacgta aaatttgccg atgaggccgt ttgtatcggc
     5701 cgaccgcaaa gcggggactc ctatctgaat attcctcacc tgatggcggc ggcagagatc
     5761 accaatgcgg atgccattca tcccggttat ggcttcctgg cggagaatgc caagtttgcc
     5821 cagatctgta atgatcatgg gatcaagttt atcggtccca caccggatat gatcaatgcc
     5881 atgggtgata agattacggc taaagaaacc atgattaaag ccggcgtgcc tgtggttcca
     5941 gggggagaag gcttgttaca aagtgtggat gaagcaaaag ggctggctaa aaacatgggt
     6001 tatccggtga ttcttaaagc gactgccgga ggtggtggca aggggatgcg gattgtttgg
     6061 gaagaatctg aaattgaaaa agcatacgat actgccaaag cagaggccgc cgcagctttt
     6121 aagaatgacg gcatctacat ggagaaattt gtggaagagc cccgtcatat cgagatccag
     6181 gttgccggtg accaatatgg tacggtttgt cacctgagtg aaagggattg ttctatccag
     6241 cgccgtcacc agaagctggt ggaagaatca ccttcgcctt ttatgacgga cgaactccgt
     6301 tacaaaatgg gagaggccgc caagaaagcc gccggcgcca tcaattatga aagtgtagga
     6361 acgattgagt tcctcgtgga taagcaccgc aatttttatt tcatggaaat gaatacgcgg
     6421 atccaggtgg agcattgtgt aacggaagaa gtgatcaact tcgacctgat caaagaacaa
     6481 attctgatcg cggctggtca tcgtatttcc ggtaaagatt atatccctca gatgcactct
     6541 attgagtgtc gcatcaatgc cgaggatccg tataatgatt tccgtccatc acccggtaag
     6601 atcaccgtgc tgcatcagcc gggtggtcat ggggtacggg tggacagtca tgcgtatgcg
     6661 ggctatgtga taccaccgta ttatgattcc atgatcggta aactgatcac ggtggcgcag
     6721 acccgccagg aagccattca gaccatgtac cgcgctttga gtgagtatgt gattgaaggg
     6781 gtgaagacca cgattccgtt tcacctgcag ctgatgcagg atgagcgttt ccggagcggg
     6841 gatttcaata cgaagttttt agaagggttt acgctgaggt gagtgctgtt ggtgataaca
     6901 ccaacagctg acagctgatt ataggtgagg tacctatagc agatagagtg tagttggtgt
     6961 tatcaccaac aacacttagg ctgttggtga taacaccaac tgatcttgtt ggtgataaca
     7021 ccaacaagac tcagtcccac cacaaaaaga tacttcccgt cctcctgagt tcatcttcca
     7081 gtgcctcgag ggtttcagtc ccctggtcta ccacatcggg gcagaactgg taaatctctg
     7141 cggccagctt attgtaatca atctctttat tgacgattgc gcattcacag aagtcaaagc
     7201 cgataccggt gatccggaca ccaaagctgg catcccattt tttcaattgt tcaataatat
     7261 ctgcggtcat cagctcatga ttggccccgt tggtctcggt cagttgcatg atcaaatagg
     7321 gatcggtgat accgttcagg atagccacct gtaatccgtt gccggggata tcactgatat
     7381 aaaggaattg tcctttgctg ataaattcct gctggtactg gcagatgatc gcgcgggctt
     7441 cttcttcggg acagaagctg cagataccgc tgggtttatt cgtctccgta tcataatctt
     7501 cgaagaccag tttttcaatg ggtcgtttgg tcagtttttt caggaaaatg ccggtatccg
     7561 gatgaagccc ggctttgttg cagtagccga tttcaatgga ggtcagggcg gagggatccg
     7621 ttacttctgc gggtagtgca gccggttttt cttccggaac ttctttcgga ggaacggccg
     7681 cttttttcag cagggagaaa gcttgtttga agaaaccact acgatccata tcttgttggt
     7741 gttttcacca acaagatacg atgctttgct ggtgaaaaca ccagcaaaga acgggatcac
     7801 tcccttaccc tcgtatcgat aataatcttc ttccggttca ggtattttcc gaaagtgagt
     7861 ccgaagactt tgtgtttgat ggaaggtccg ccatcgcggt tgctgaggtt ggcaggacta
     7921 tgggtgaact ggaagtagac gatgaagcca ccgtcttttt cgtaaccaag ctgactaatc
     7981 aggttggcgg aatagcggcc gtattcgccc ggaccgaatt tcatattgcg gctgacggaa
     8041 ccgccgccac tcaggttaaa tttttctttg gcgaataaca taaagtccag ggccgggccg
     8101 gctttaataa acacatgcga aggcttgggg ctcaggtcaa attgcagcaa tgcggccagt
     8161 tccatcgtat ggaaacgggt gctgttgtca atggcatcaa tatcaggtaa agaattgggg
     8221 aggttatagg ttacatcata ccctttatag ctgtaataga ctgatggtgc gaaatagaga
     8281 aatccatcaa agggtacttt catgccaatg cctgcatgca atccgtactt gaagccggtt
     8341 tcctgtttct ggttacggat ggaataattt gcggtactca tttgcggacc ggcaaaaagc
     8401 gctacctgat tctgggcgga tacggtaagg ccggcaaata ataaaacaat cagaataata
     8461 cggttcatgt ggtggttgta attcagtttc agtaagttat tcagcttttc aaggatgatc
     8521 agcggtctga acagtagcaa ttggatatgc gggggctaaa atagagaata aatggcggga
     8581 atacaaggca aaatacattt tcccgcagat ggacgcagat ttccccacag ataggcgcag
     8641 atggatcctg atgttttcat cggaaacttt gttgtatttc tttttgttga ttttaataca
     8701 aagtacattt tcccgcagac gggcgcagat aatcacgcag atggcgcaga tttaatctcc
     8761 attcgatgtg gatttccaca gtttgaacct gttattttca ccggatattc tgttttattt
     8821 cctatggtag ttggaagagc aggtttgaat tttggaggta taaaaattat gagccatgca
     8881 tgaaaatgac atttcttttt taatacggaa agcgattttt acagtatata ataccctggg
     8941 accgggttta tatgaatcgg tgtatataac cgcacttcgt catgaacttg aaaaattggg
     9001 attaatggtc agtgttgaaa agccgctgcc cttaatttac gacggaatca gattcgaagc
     9061 gggcttccgc attgatatga tggtgaatga acgagtgatt attgaggtga agtctgttga
     9121 aaaactggca gaagtacatc ataaacaaat tatcacttat ctgcgtctct c
//
LOCUS       DAOPMW010000101         9102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8373_length_9102_cov_9.444567, whole genome shotgun sequence.
ACCESSION   DAOPMW010000101 DAOPMW010000000
VERSION     DAOPMW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_9102_cov_9.444567"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1351)
                     /locus_tag="PLU11_06645"
     CDS             complement(164..1351)
                     /locus_tag="PLU11_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN58831.1"
                     /translation="MAYRKFKADRLFDGDRFREGEILITDEKGKVEAIVPESEAGENT
                     EQVAGILCPGLINSHCHLELSHLKNVIPPHTGLIDFLCSVVTKRGFARELIDAEIIRA
                     EKEMEANGIVAVGDIGNTADTAAVKSSSRIRWQNFVEVLSFTDEKAVENFAHYKQVAE
                     AMKESLKDSPVSHRTSLVPHAPYSISPDTFRMINEATSGQVISMHNQEHPAEDELYKT
                     GGGEYLRFFKIFGMNQSPFPVTGKSSIRSVLPYFTNGQTIFLIHNTFMSEEDIIWANE
                     YAANNGLKLVYCLCINANLYIENKVPPVDLLMKHQCDMVLGTDSYSSNWQLSIAKEME
                     AVKKYFPHIPDATLLQWATRNGAQAFGWNDQLGSFRKGISPGLVRLEADLSNSARLQM
                     SNQ"
     gene            1457..2521
                     /gene="prfA"
                     /locus_tag="PLU11_06650"
     CDS             1457..2521
                     /gene="prfA"
                     /locus_tag="PLU11_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPN58832.1"
                     /translation="MLDRLEAIKARFDQLGIALTNPEIVSNNKKFAETSKEYRSLEKI
                     VQAYLDYRKILDDIEFYKEALGGNDEEMRELAKMEMPVMEENKVKAEADIRQLLIPKD
                     PQDEKNAILEIRAGTGGDEASLFAGDLLRMYVRYCERRGWKTAILSENEGTVGGYKEV
                     QLEVTGDDVYGTLKFESGVHRVQRVPDTEGSGRVHTSAATVAVMPEAEEVDFELNPAD
                     VKMETARSGGAGGQNVNKVETKVMLTHIPTGTVVICQTERSQLGNREKAMGMMRTKLY
                     EEQVRKQEDEIARHRKSLVSTGDRSAKIRTYNFPQGRVTDHRIGLTLYNLDAVLNGDI
                     QELIGALQFAENSEKLTKQN"
     assembly_gap    2816..2915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2916..3512
                     /locus_tag="PLU11_06655"
     CDS             <2916..3512
                     /locus_tag="PLU11_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58833.1"
                     /translation="VKNFGKDTVVENPEIPNEQNSEVLTDATKTITSGFQNVLAGGGL
                     ENIMDLFKGGGTSGGSGVSGLLKNPMVTMMVGHFISKLVCKYNMTPASASSVANNLIP
                     NVLNGMITETNNPANPGFTLDGLIGSLTGGNGGGSQLQDILSQFTGGGSTSGGNGGGF
                     DLQDLIGNFTRKAQDNFQPSGDTGGGGLMDMIKGFLSK"
     gene            complement(3922..4299)
                     /locus_tag="PLU11_06660"
     CDS             complement(3922..4299)
                     /locus_tag="PLU11_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPN58834.1"
                     /translation="MQIRFLLPGILWIILCTILFTLPGSSFPKEDWLDKIWFDKWVHI
                     GLIGLLVVSWCWGMQKKLVNKQKLAGIFRWILFLAIAYGVGMEFVQKHLVANRSFDGG
                     DIIADSAGAIIGYLYSSRVYIKK"
     gene            complement(4467..5228)
                     /locus_tag="PLU11_06665"
     CDS             complement(4467..5228)
                     /locus_tag="PLU11_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HPN58835.1"
                     /translation="MNIQEYISGGIIESYVLGLASPEERAEFEQYCSQYPELVEARIA
                     FEISLEQQALSGTTKPPAFIKENILQEINKGAKVIPFKPVYGRSANLWKYTAAAAVLL
                     LAGSITWNISQMNQNKTLSSNLDKANENIMGLVAEIGKLQQNPNIKMASLKGMDASPQ
                     SFATVYWDTASHDVFLLANNLPAPPTDKQYQLWALLDGKPVDLGMMEYNIRQKKLLVR
                     MKNVGNAQAFAITLEKMDRPDPSKPGGDMYVMGKL"
     gene            complement(5233..5772)
                     /locus_tag="PLU11_06670"
     CDS             complement(5233..5772)
                     /locus_tag="PLU11_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN58836.1"
                     /translation="MNLNTTYSEQELVSALRLRDEKAFAYLYDQYSGALYSVVNKVLP
                     DKELANDVLQTVFINIWRRFDSYDPSKGRLFTWMLNIARNAAIDEMRSKGFNNNRKNQ
                     PLPESNEVAGAVTGPAIKDSGLSKVIGQLKEEWRVLLDLAYYQGFTHEEIAKVMDLPL
                     GTVKTRIRAALIHLRTLLQ"
     gene            complement(5847..6224)
                     /gene="gcvH"
                     /locus_tag="PLU11_06675"
     CDS             complement(5847..6224)
                     /gene="gcvH"
                     /locus_tag="PLU11_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPN58837.1"
                     /translation="MSYPANLRYTKDHEWISLDGTTATIGITEYAQRELGDIVYVEVD
                     TIGKALEAGAVFGTVEAVKTVSDLFLPVSGTINELNPALTNAPELVNSDPYGEGWMVK
                     MTVNNPADLDGLLDAAGYQAVVD"
     gene            6417..8876
                     /locus_tag="PLU11_06680"
     CDS             6417..8876
                     /locus_tag="PLU11_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HPN58838.1"
                     /translation="MRKLVLIFLSAFTLSFSASAQKISGVVKDEQGKGLEKTTISLLR
                     SKDSTVIKLAVTGKDGSFSMESAGGSYLVSASHIGYTTRYSAVFDAKSDTRIPEIVLE
                     KAAASLQGVTVTSQKPMIEVRADKTILNVEGTINAVGNDALELIRRSPSAMVDKDDNI
                     SLAGKNGVQVYIDGKPSPLSGSDLANYLKSMQSSQIEAIEIITNPSAKYEAAGNAGII
                     NIKLKKNKSFGTNGSVNAGYTQGIYGKGTAGINLNHRNKHINVFGNYNYNKGDYLMTM
                     NSERTQFDTLFLQKNEILFRNNTHGFKAGMDYFIDNTKTIGAVVNGNLASNDLNTAGP
                     MDFIYLPTGQKDRVLKATNHNDMERDNLNANLNYRYAKTGGAELNLDADYGFFKIRSN
                     QFQPNYYYLANGTTETSRAIYNMIAPTDIDLYSVKADYETNYKGGRLGVGGKIGVVES
                     DNDFQRYDVYTNGKVLDTLKSNRFKYKENINALYVNYNKQLKKGIMIQAGLRAENTNS
                     DGNSTGFKEVNNVMVPYDSTFSRNYTDVFPSAAITFNKNPMKQWTFSYSRRIDRPAYQ
                     DLNPFEFKLNEYSFMKGNTRLQPQYTNSFDVTHVYKYRLTTKLTYSHVKDIFAQIPDT
                     IDKTKSFMTKKNLATQDVVAVNISYPFQYKKYSFFASLNTNYSHYVADFGGGDRKVNQ
                     EVFSLTYFMQNSYSFGKGWTGELSLLYISPSVWQGLIRSDAMGSVDAGVQKVIFKGKG
                     NVKLAMSDIFRTMKWGGDTNFAGVENRFTGNGEMQQVKLNFSYRFGNSQVKAARQRKS
                     AIEEERKRAEGGGQQGGMGNQ"
BASE COUNT         2534 a         1967 c         2133 g         2368 t
ORIGIN      
        1 tttaaagaag aaatgagtaa tgagtaatca gtaatgagcc tgcctgccgg caaaggcagg
       61 tagctgagcg atttttacaa aatcattgta catcccgctt tgcgaaaagt accaattcta
      121 taacgatttt ccccaatact cattactcat tactgattac tcattattga ttgctcattt
      181 gcaatctggc cgaattgctt aaatcagctt ccaaccggac caatccaggg gatatgccct
      241 ttctgaaact tcccagctga tcattccatc caaaagcttg tgcaccattt cgggtggccc
      301 attgcaggag cgtggcatcc ggaatatgcg ggaaatactt tttgacagcc tccatttctt
      361 tggcgatact cagctgccag ttactactgt aactatctgt acccagtacc atatcacact
      421 gatgcttcat cagcaggtcg acaggcggta ctttattttc aatatacaga ttggcattga
      481 tacaaagaca atagaccagt ttcagtccgt tgttggcggc atattcattt gcccagataa
      541 tatcttcttc ggacatgaag gtattatgga tcaggaaaat ggtttgaccg ttggtgaaat
      601 aaggtaatac ggagcggata ctgcttttgc cggttaccgg gaaaggagac tgattcatcc
      661 cgaagatttt aaaaaaccgc aggtattccc ctccgccggt tttatagagt tcatcctctg
      721 ctggatgttc ctgattgtgc atcgaaatta cctgtccgga agtagcttca ttgatcatcc
      781 ggaaggtatc gggtgaaatg ctatatgggg catgaggcac cagggaggta cgatgtgaaa
      841 ccgggctgtc tttcaacgat tctttcattg cttccgcaac ctgcttgtag tgagcgaagt
      901 tttcaacggc tttttcatcg gtaaaactca acacctccac aaaattctgc caccggatcc
      961 ggctactgct tttcacggcg gctgtatcgg cggtatttcc aatatccccg acggctacaa
     1021 taccattcgc ttccatttcc ttttctgccc ggatgatttc cgcatcgatc agttcgcggg
     1081 caaaaccccg tttggtgact actgaacaga gaaaatcaat aagaccggta tgcggaggga
     1141 ttacattttt caggtgactc aattcaaggt ggcagtggct gttgatcagt cccggacaaa
     1201 gaatgccggc tacttgttca gtattttcgc ctgcttcact ttccggtacg atcgcttcca
     1261 ctttcccttt ttcatcggtg atcagtattt ctccttcgcg gaatcggtcg ccatcaaaaa
     1321 gacggtcagc cttgaatttt cggtaagcca tgccgcaaaa ttgccaaatc cgatttccaa
     1381 ttcccaaaaa attattcttg ttacctttgc cctcctttat atataaaccg gatttaccgg
     1441 cacagcgcac ccaattatgt tagacagatt agaagcgata aaagcccgtt ttgaccagtt
     1501 aggcatcgcc ctgaccaatc cggagattgt gagcaataac aagaagtttg cggaaaccag
     1561 caaggaatac cgcagcctgg aaaagatcgt gcaggcctat ttagactacc ggaagatcct
     1621 ggatgatatc gaattttata aggaggccct gggcgggaat gatgaggaaa tgcgggagct
     1681 ggccaagatg gaaatgccgg tgatggaaga aaacaaggta aaagcagaag cggatatccg
     1741 tcagctgctg ataccgaaag acccccagga tgaaaagaat gccatcctgg agatcagggc
     1801 cggaacgggt ggtgacgaag ccagtctttt tgcgggtgac cttttacgta tgtatgtccg
     1861 ctattgcgag cgcaggggat ggaagacggc cattctgagt gaaaatgaag gaacggtggg
     1921 cggttataaa gaagtgcagc ttgaagtaac cggcgatgat gtatatggaa ctttgaaatt
     1981 tgaaagcggg gtacaccggg tacagcgggt gcctgataca gaaggttcag gccgggtaca
     2041 tacttccgca gctaccgtgg ccgtgatgcc tgaagcggag gaagtggatt ttgaactgaa
     2101 tccggctgat gtgaaaatgg aaacggcccg tagtggtggt gcgggcggac agaatgtaaa
     2161 caaagtagag accaaggtaa tgctgacgca tattcctacg ggtacggtgg tgatctgcca
     2221 gaccgaacgt tcacagctgg gtaaccgtga gaaagccatg gggatgatgc ggacgaaatt
     2281 atatgaagag caggtgcgta aacaggaaga cgaaattgcc cgtcaccgga agagtcttgt
     2341 gagcaccggt gaccgcagtg ccaagatccg tacctataat tttccacagg gacgggtaac
     2401 cgaccaccgg attgggttaa ctttatataa tctggatgcc gtgctcaatg gcgatatcca
     2461 ggaactgatc ggggcccttc aatttgcgga gaattcggaa aagttaacaa agcagaatta
     2521 ggatgatccg tgtagcagtg ccttcggcaa aggatttgcc ttcggcaaaa aaggtagcca
     2581 cgaatgtgca ctaaagtgca cgaaaaatac agggccacgg atgacacaga tttacacgga
     2641 tcaagatata tctctctgga ggggctgttt cctgacatct ggcaactgac atccgacacc
     2701 taacaacgaa cacctaacac ctaacaccta tattttatgc ttgaacaatt aatggacctt
     2761 gtaaagaatt tcggaaaaga cacggtagtg gaaaatcctg aaataccgaa tgaacnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtaaa gaatttcgga aaagacacgg
     2941 tagtggaaaa tcctgaaata ccgaatgaac aaaacagtga agtgctgacg gatgccacca
     3001 aaaccatcac cagtggtttt caaaatgtac ttgcaggcgg cggactggag aatattatgg
     3061 acctttttaa gggtggcggc acttccggag gatcgggtgt ttccggatta ttaaaaaatc
     3121 cgatggtcac catgatggtg ggacatttta tcagcaaact ggtgtgtaaa tataatatga
     3181 cacccgcgtc ggcgagttct gttgctaata atcttattcc caatgtgctg aacggaatga
     3241 tcaccgagac caataatccg gctaatcccg ggtttacact cgatggactt attggttctc
     3301 ttacaggagg aaatggaggc ggctcccagt tacaggatat cctgagtcag ttcaccggtg
     3361 gcggttccac atcaggcggc aatggcggag gatttgactt acaggatctt atcggtaatt
     3421 tcacccgcaa agcccaggat aacttccagc cttccggcga tactggcggc ggcggattaa
     3481 tggatatgat caaaggtttc ttatcgaaat aagcggaact ccttaccgaa ttaagctatt
     3541 cttaaccgga tagctttttt tttgactcaa tttggcacga aatttacatt agaattgtca
     3601 gcaagtgatt gtctgttctt tgtcagctga ttgtcaggtt ttttgaaaga aaatttcgta
     3661 taaactaaca agtgtttttt gttgtttatg caataagtaa ggtgattgca tcgtattagt
     3721 gaagagaaac ctggaaaatg tacgttgcgg atctgaagtg attttacgga ttctgttctt
     3781 ttaaactttc gttaacagca acattcatac tacatgcagt acatttgctt tatagatata
     3841 gaaattcaga actcattata aagacagtca attttctcat aagcagttag ttttggttgc
     3901 gacccctgtt tccacagggg tctatttttt tatatatacc ctgctgctat acaggtagcc
     3961 aataatggca ccggccgaat ccgcaataat atcccctcca tcaaaggagc gattggccac
     4021 caggtgtttt tgtacaaact ccattcctac accataagca attgccagga ataagatcca
     4081 acggaatata ccggccagtt tttgtttatt gaccagtttt ttttgcatac cccagcacca
     4141 gctgaccacc agtaaaccga tcaatccgat atgtacccat ttatcaaacc agatcttatc
     4201 cagccagtct tctttcggaa aggaggaacc cgggagtgtg aaaagaatcg tacaaaggat
     4261 aatccataaa attccaggca ggagaaagcg aatctgcatc gtttaactta tttcggcaat
     4321 attagcgagt ttttgacagc tttaaacata aacagggagc cctgaagagc cccctgttct
     4381 taaccaacta tagaaccaac atgactatag caatagctgt ctggttatat acgtaaagga
     4441 acaggggaaa gattggaaaa attgaatcag agtttaccca tgacatacat gtcgccaccg
     4501 ggctttgatg gatcaggacg atccattttt tccagtgtaa tggcaaatgc ctgagcatta
     4561 cccacatttt tcatccggac gagtagtttt ttctgacgga tattgtattc catcataccc
     4621 aggtcaacag gtttgccatc gagtaaggcc cagagctggt attgtttatc cgttggcggg
     4681 gccggcagat tatttgccag caggaataca tcatgggagg cggtatccca gtaaacggtg
     4741 gcaaaggact gaggcgaagc atccattcct ttgagtgaag ccatttttat attggggttc
     4801 tgctgcagtt ttccgatttc tgccaccagc cccataatat tttcattggc tttgtccagg
     4861 ttggaggaaa gtgttttgtt ctggttcatc tggctgatat tccaggtaat acttccggcc
     4921 agcaacaaaa cggcggccgc tgcggtatac ttccataggt tggccgatct gccatagact
     4981 ggtttaaagg gaatgacttt tgcgcctttg ttgatctctt gcaggatgtt ttctttgata
     5041 aatgccggcg gcttggtggt tccggacagg gcttgctgtt ccagggatat ttcaaaggca
     5101 atacgggctt ctaccagttc ggggtattgg gaacaatatt gctcaaactc cgcccgttcc
     5161 tccggactgg ccagtccgag tacgtagctt tcaataatgc cgcctgatat gtattcctgt
     5221 atattcaatg cgttattgta gtaaagttct taaatgtatt aatgccgccc ttattcttgt
     5281 tttaaccgtg cccagcggaa ggtccatcac tttagcgatc tcttcatggg taaatccctg
     5341 ataataggcc agatccagca aaacccgcca ctcctccttc agttgtccaa tcactttact
     5401 gagtccgctg tcctttatag cgggccctgt tacagccccg gcaacttcat tactttccgg
     5461 cagtggctgg tttttccggt tattattaaa tccttttgac cgcatttcgt caatggctgc
     5521 attccgggca atattcagca tccaggtaaa cagccttcct ttggatgggt cataggaatc
     5581 gaaacgacgc cagatattaa taaaaaccgt ctgcaaaaca tcattggcca gttctttatc
     5641 tggaagcact ttattcacga cactgtacag tgccccggaa tactgatcat acaggtaggc
     5701 aaatgctttt tcatcacgta accgcaatgc agacaccagc tcctgttcac tgtatgtagt
     5761 gttgagattc aaataattgg ttaagataca acgagaataa gccgctttgt atcgccttta
     5821 tatacgggag attgtcccgg tcggatttag tcaaccactg cctggtagcc agccgcatcc
     5881 agtaatccat ccaggtcagc cggattattg acggtcattt taaccatcca gccttcaccg
     5941 tatggatcgg aattcaccag ttcgggtgca tttgtaagtg caggattaag ttcattgatc
     6001 gttccgctta ccggcagaaa caggtcagac acggttttaa ccgcttcaac ggtgccaaaa
     6061 acagcacccg cttccagtgc tttaccgatc gtgtcaactt ccacatatac gatatcaccc
     6121 agctcacgtt gtgcatattc agtgatgcca atggtggcgg tagtgccgtc taaactgatc
     6181 cattcgtggt ctttggtgta gcggagatta gcagggtaac tcataatcaa tggttttgag
     6241 ttgcaaacct aaggaaaatc gggaaaagaa aaggaagcag gataaagtta ggctgattat
     6301 ttcaccggaa tatttatgcc gttcatggca agatttgcgc cattggtcgg gaaacgcttt
     6361 tacagacgta taacatgccg ttatatttgc cattcaattc acaaaaacca ttaataatga
     6421 gaaagttagt attgattttt ttgagcgcct ttaccttatc cttttcggct tctgctcaga
     6481 aaatatcagg tgtggttaaa gatgagcaag gaaaagggct cgagaagaca accatctccc
     6541 tgctccgttc aaaagattcg acagtaataa aactggccgt tacaggcaaa gacggcagct
     6601 tttccatgga atctgccgga ggcagttacc tggtaagtgc ctcacatatc gggtatacga
     6661 cccggtattc agcggtgttt gacgcaaaat cggatacccg gattccggaa atcgtactgg
     6721 agaaagcagc tgccagtctg cagggcgtaa ccgtaacctc acaaaaacca atgatcgaag
     6781 tacgggccga caaaacgatc ctcaatgtgg aaggaaccat taatgctgtg gggaatgacg
     6841 cgcttgaact gatccgccgt agtccgagtg ccatggtgga caaggatgac aatatcagcc
     6901 tggccgggaa aaacggggtg caggtctaca ttgacgggaa accttcaccg ctttccggtt
     6961 ccgatctggc caattacctg aagagtatgc aatcttccca gatagaagcc atcgagatca
     7021 ttaccaatcc ttcggccaaa tatgaagcgg cggggaatgc aggtatcatc aatatcaaat
     7081 tgaaaaagaa caagagtttt ggtaccaatg gatccgtgaa tgccggttat acacagggga
     7141 tttatggaaa aggcaccgcg ggcattaacc ttaatcaccg gaataagcat atcaatgttt
     7201 ttggtaatta caattataat aaaggcgatt acctgatgac gatgaattcc gaacggaccc
     7261 agtttgacac cttgttttta cagaagaacg aaattctgtt ccgcaataac acccatggat
     7321 ttaaagcagg gatggattac tttatcgaca ataccaaaac catcggtgct gtggtgaatg
     7381 gcaacctggc cagcaatgac ctgaataccg cgggtcctat ggattttatt tacctgccta
     7441 ccggccagaa agaccgggtg ctgaaagcca ccaatcataa tgatatggaa cgggataacc
     7501 tcaatgccaa cctgaattac cgctatgcga agacaggagg tgctgagctc aacctggatg
     7561 cggattatgg ttttttcaaa atccgttcta accagtttca gccaaactat tattacctgg
     7621 ccaatggaac caccgaaacc agccgggcta tctataatat gatcgctcct actgacatcg
     7681 acctgtattc ggttaaggct gactatgaaa ccaattacaa aggcggacgc ctgggtgttg
     7741 gcggaaagat cggggtggtg gaatcggata acgatttcca gcgctatgat gtgtatacca
     7801 atggcaaagt gctggatacc ctgaaaagca accggtttaa atacaaggaa aatatcaatg
     7861 cactgtatgt gaactacaac aagcagttaa agaaagggat catgatccag gcaggtctgc
     7921 gtgcggagaa tacgaattca gatggcaact ccaccggttt caaagaagtg aataatgtaa
     7981 tggtgcctta tgactctacc ttcagcagga actataccga tgtatttccg agtgccgcga
     8041 tcacgtttaa taaaaacccg atgaagcagt ggacgttctc ttattcccgc cgaatcgatc
     8101 gtccggcata ccaggacctg aatccatttg agttcaaact gaatgagtat tcctttatga
     8161 aaggaaatac cagattgcaa ccgcaataca ccaacagttt tgatgttacg catgtataca
     8221 aataccggct gaccactaaa ctgacttaca gtcatgtaaa agatatattc gcgcagattc
     8281 cggataccat tgacaagacc aagagtttta tgaccaagaa gaacctggcc acgcaggatg
     8341 tggtggctgt gaatatcagc tatcccttcc agtataaaaa gtacagtttc ttcgcctcac
     8401 tgaatacgaa ctattcgcat tatgtggcgg actttggtgg cggcgaccgg aaagtgaacc
     8461 aggaagtttt ttccctgact tatttcatgc agaacagtta cagttttggt aagggctgga
     8521 ccggtgaact gagcctgctc tatatatcac cttccgtatg gcagggactg atccgttcag
     8581 acgccatggg aagtgtggat gccggtgtgc agaaagtgat ttttaaagga aagggcaatg
     8641 tgaaactggc gatgagtgat attttcagga ccatgaaatg gggaggagat accaattttg
     8701 ccggagtcga gaaccgcttt accggaaatg gcgaaatgca gcaggtaaaa ctgaacttca
     8761 gttatcgctt tggtaacagc caggtaaaag cagcccgtca gcgtaagtca gccatcgagg
     8821 aagaaagaaa gagagcggaa ggtggcggac agcagggagg aatgggtaat cagtaagtag
     8881 ttagtagtcg ggagttagta gtcaatacac ctcctaagag cttatagtaa aagagcatgg
     8941 aaaatctgtg ctcttttact tttatataga tgtcagctgt caggtgtcgg gtgtcaggaa
     9001 acagcccatc ctgagcaagt tagtagttag tagcctgcct gccggtagtg tattacttca
     9061 caatttcggt aacagatttg caattttttt tagtttatct aa
//
LOCUS       DAOPMW010000102         9017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8486_length_9017_cov_9.559250, whole genome shotgun sequence.
ACCESSION   DAOPMW010000102 DAOPMW010000000
VERSION     DAOPMW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8486_length_9017_cov_9.559250"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..506
                     /locus_tag="PLU11_06685"
     CDS             132..506
                     /locus_tag="PLU11_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58839.1"
                     /translation="MKRILLMAGLLLSLSGWSQQKDWKEYRDFYDLVVLILHPAEYGE
                     LNSVKDSANLLLEKAIAWQASPVPSRYKKAEQIKKGLIELTTFCRELQDAVIQKKDDE
                     IISLLAIKTHNKFHAVSGRYML"
     gene            534..1340
                     /locus_tag="PLU11_06690"
     CDS             534..1340
                     /locus_tag="PLU11_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58840.1"
                     /translation="MKSFILFTTFLFLLVSCNRKMAAGNNPETASSTLTLRFENSAGN
                     QRVAMNGDRYTNATGEAFSVTLLQYFISNISLKKTDGSVITLPQDSSYFLVKESVPES
                     KQITLTVPKGNYASVSFMLGIDSLRNTKPLAERTGILDPAGGAEGMYWGWNSGYIFFK
                     MEGHSAAIPDNKAGTKKFRYHIGLYGGMNSPTVNNIKTITLDLKDKQVAKAGPGKKPV
                     VIINTNILQMFTGTENISIAKNPVVMVSPFSKYIADNYASMFSLVAVDNQ"
     gene            1351..2412
                     /locus_tag="PLU11_06695"
     CDS             1351..2412
                     /locus_tag="PLU11_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPN58841.1"
                     /translation="MKKTFLSKRLVTSLGLFLAIALAASFTVRQELFHQPANFPDPVY
                     NTGANPVTDAGFTLGKKLFYDPILSADYTISCGSCHIQAAAFSHHGHDLSHGIHDLLG
                     TRNAPGLQNLAWYSRYMWDGGITNLDLQPIAPITNPVEMGDTLSAVMRKLNSHPQYPG
                     LFEKAFGTDSITTDLVMKALSQFMISLVSADSKYDKVMRKEGASFSPDETLGFQVFQE
                     KCASCHPEPLFTDQQFRSSGLPILEEGDEGRYLVSLDSNERFTFRVPSLRNLKFTFPY
                     MHDGRFYTVDEVLDHYASGISHATRPDPAFKLPDGSYGIPVSPDQRKALKAFLLTLND
                     TAFVKNPLYDEAGSQLYIK"
     gene            complement(2483..2809)
                     /locus_tag="PLU11_06700"
     CDS             complement(2483..2809)
                     /locus_tag="PLU11_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPN58842.1"
                     /translation="MTIQEAQQQVDNWIKTTGVRYFSELTNMTILTEEVGELARLMAR
                     TYGDQSFKESDKNRDLGDEMADVLWVLICLANQTGVDLTQALEKNFEKKSVRDADRHK
                     NNEKLQ"
     gene            2974..3315
                     /locus_tag="PLU11_06705"
     CDS             2974..3315
                     /locus_tag="PLU11_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58843.1"
                     /translation="MWKSIQRLLALLSLSLFINAAAAQPATNSFDRSFKKEIKPFKIL
                     TSGKQITIKSTRDIKTIMVWTATGHRIVEQTLVNASSYTFNVSGSRERIFFVMVQYEG
                     QKPVTEKFGVE"
     gene            complement(3505..3957)
                     /gene="dtd"
                     /locus_tag="PLU11_06710"
     CDS             complement(3505..3957)
                     /gene="dtd"
                     /locus_tag="PLU11_06710"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPN58844.1"
                     /translation="MRAVIQRVSRASVTIGGKVHAQIGWGLLVLLGIEDADTEEDIQW
                     LSSKIVNLRIFDDAGGVMNESVKDRDGDIILVSQFTLHAATKKGNRPSYIKASKPETA
                     IPLYEKIITQLTADLGKPIGTGVFGADMKVELLNDGPVTIVIDTKAKE"
     gene            4125..4532
                     /gene="arfB"
                     /locus_tag="PLU11_06715"
     CDS             4125..4532
                     /gene="arfB"
                     /locus_tag="PLU11_06715"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPN58845.1"
                     /translation="MFHSLLPEISFQTTRSGGKGGQNVNKVETAVIVAFHINSSALLS
                     GEQKQLLMEKLSNRINSEGYLQVKAQTHRTQLANKEEAVKKIHELVSKALEKKKARIA
                     TKVGKAAKERRLESKKRKADVKSGRQKFRPGDF"
     gene            4572..5618
                     /locus_tag="PLU11_06720"
     CDS             4572..5618
                     /locus_tag="PLU11_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPN58846.1"
                     /translation="MRRKLITISTLILFVAGFSVIPVACKKEQSTRGLEKLALEIPDS
                     FPDPAYAFTDNPLSKEGFELGRRLFYDPQLSADGLVPCSSCHQQIAGFGTFEHDRSHG
                     VFNSHTLRNAPVLFNLAWNTSFHWDGEFTSLADEAAHPLTGSNEMGQTYQRIIDYLEA
                     DRTYRDAFKEVFRYPFIRPEFINKALAQFTGSIVSANSKFDKYKKGTVNFSAQEESGY
                     QLYKVNCASCHPEPLFTDYSYRNIGLRIDPLLNDYGRLRVTGKAEDSLKFKVPTLRNT
                     YISSNYMHDGRFNTLLQCLNHYRNNVQQGTTLDPLLVNGISLSDTQTDNLLLFLRALT
                     DSSLLRDPRFTKPD"
     gene            complement(5615..6259)
                     /locus_tag="PLU11_06725"
     CDS             complement(5615..6259)
                     /locus_tag="PLU11_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58847.1"
                     /translation="MKQLILSLLMISGLNLVLSAQAYEGTADYDKKKQQVFMIDYPYP
                     PEAVENAVIKKMSKLGYKSKEEKGLFNKDKGYRNFKDAFITEISADKMDYIIKVESKG
                     KKSDASVVYLIIMKEGNNMKTSFDAYQVDRVKAFLNSLLPDVEAENLELDIIAQEEAV
                     NKAEKKLRGLKDDQSDLEKKLKNNQEDQRDTEKEIESKKQALEALRGRRVNNKL"
     gene            6419..7933
                     /locus_tag="PLU11_06730"
     CDS             6419..7933
                     /locus_tag="PLU11_06730"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPN58848.1"
                     /translation="MSGIKKLAGQTLWYGVPTIASRFLGYLMNLTLPLIFAQPSKTAD
                     LTQVYAIIPFLNVLFTYGLETAYFRFSQEQDQKKLYNTLSLSLFGSTILFTGALFLFK
                     DTIASWASLENHPEYINWMAGIIFFDTISTLAFARLRQENRPKRYAFARLASVLMNIL
                     VVVLFLGIVPRMAADSPDSFWGVLHSDKDTGIVYYLIGNFCGSVLTFLILQKELRQVH
                     FQFDNALWKKIMQYSYPLVIVGMGGIVNDMLSRLIYQHVVDLPAEQAKHELGVFANII
                     RLSILITIAIQAFRMAAEPFFFNRSKEENAPKTYARVMKFFVLVCCFLFLGISLYIDI
                     VKWFFLAIDRSEWTEGLYVVPLFAMANIFLGIYYNLSIWYKLTNKNMMGAVITLAGAV
                     ITIALNILLIPKLHYLGAAIATFCCYLFMMIISYTQGQKHYPVPYAKKKLIAYLVLVT
                     LLVLTHRGFVYFVDNRWANLAIATLLLGMFTWFVGKIERKELEKLPVIGKFYRK"
     gene            complement(8124..8762)
                     /locus_tag="PLU11_06735"
     CDS             complement(8124..8762)
                     /locus_tag="PLU11_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN58849.1"
                     /translation="MLVVKSFTFSPVQENTYVLYNEQKQCCIIDPGCYFPAEKDELKT
                     GIEKAGLTPVLLLNTHCHLDHVFGNKFVHDTWGLDLHIHEKEKLVLDFAPVSGERWQL
                     PFDNYEGPLVYLKEGNFIKIGEDELEIRFTPGHSPGSISFYSAENGFIIGGDVLFNRS
                     IGRTDLPGGSMETLMNSIRTQFFTLPDDTKVYSGHGPVTTVGYERGNNPFVR"
     gene            complement(8829..>9017)
                     /locus_tag="PLU11_06740"
     CDS             complement(8829..>9017)
                     /locus_tag="PLU11_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58850.1"
                     /translation="RQAIRLRTFFIDYDLLFKILKSFKSLLWQPAFYNDEALAGTSNA
                     GSAVIGRDPGSKVAILYF"
BASE COUNT         2375 a         2236 c         1930 g         2476 t
ORIGIN      
        1 cattatgaca ataatgatgc tgatacgctg cgggcaataa aaaggctgcg cagtctgatc
       61 agtatcgtcc tggtattaat tgcaggtaca ataggctata tcgcctggac cttttataat
      121 taaatcaacc gatgaaaaga atactgctaa tggccggcct gttactatca ctgtcaggct
      181 ggtctcaaca aaaagactgg aaggaatacc gggattttta tgacctggtg gtactgatac
      241 ttcacccggc agaatacggg gagctgaatt cagtaaaaga cagtgctaac ctgttgctcg
      301 agaaggcaat tgcctggcag gcatcgccgg taccttcccg ttacaaaaaa gcagaacaaa
      361 tcaaaaaagg gctgatcgaa ctgacaactt tctgccggga actgcaggat gcagtgattc
      421 agaaaaagga tgacgagatc atcagcctac tggccataaa aacgcataac aaattccatg
      481 ctgtttcggg ccggtatatg ttataacatg accatttaaa aaaaataaac gcaatgaaat
      541 cttttatact ttttaccact ttcttattcc tgctggtttc ctgtaaccgg aaaatggcag
      601 ccggtaataa cccggaaacc gcctctagta ccctcaccct ccgctttgaa aacagcgcgg
      661 gtaatcaaag agtggcaatg aacggagatc gctataccaa cgcgaccggg gaggcgtttt
      721 ccgttaccct gttgcaatac tttatcagta atatttctct taaaaaaacg gacggctcag
      781 tcatcaccct gccccaggat tcttcatact tcttagttaa agaatctgtt ccggaatcca
      841 aacaaatcac gcttactgtt ccaaaaggga attatgcatc ggtttccttt atgctgggta
      901 ttgacagcct ccggaacacc aaaccgctgg ctgaaaggac cgggattctt gatccggcag
      961 gaggtgcgga agggatgtac tggggttgga acagtggtta tatctttttt aaaatggaag
     1021 gccattccgc tgctattccg gacaacaaag ccggtacaaa aaaattcagg taccatattg
     1081 gtctctatgg aggtatgaac tcaccaaccg tcaacaatat caaaacaata accctggatc
     1141 tgaaagacaa acaggtggca aaagcaggtc ccggtaaaaa acccgtggtt attataaata
     1201 ccaatatcct gcaaatgttt accggcacgg agaatataag cattgctaaa aaccctgttg
     1261 taatggtatc tccctttagt aagtatatcg cggataatta tgcatccatg ttcagtcttg
     1321 ttgcggttga caatcaataa ccattaaaaa atgaaaaaaa cgtttctttc taagcgtttg
     1381 gttactagcc ttggtctgtt tttggccatc gcactggccg ccagttttac agtcaggcag
     1441 gaactgtttc accaacctgc taattttccg gacccggttt ataataccgg ggcaaacccg
     1501 gttactgatg ctggttttac acttggaaaa aaattgtttt acgatccgat tctttctgcg
     1561 gactatacga tcagttgcgg cagctgtcat atacaagctg ccgcattttc gcatcatggt
     1621 catgatctga gtcacggcat acacgatttg ctgggcaccc ggaatgcccc cgggctgcaa
     1681 aatctcgcct ggtactcccg ttatatgtgg gatggaggta tcaccaatct cgatcttcag
     1741 cccattgctc cgattaccaa tccggttgaa atgggcgata ccttatctgc cgttatgcgc
     1801 aaattgaaca gccaccccca atacccgggg ttgtttgaaa aagcgtttgg caccgacagt
     1861 atcactaccg acctggtgat gaaagcgctc agtcagttta tgatctctct ggtcagcgca
     1921 gacagtaaat acgataaagt aatgcgaaag gaaggcgctt cattttcccc ggatgaaact
     1981 ctgggcttcc aggtgttcca ggaaaaatgt gcctcctgtc acccggaacc cctgtttacc
     2041 gatcagcaat tcagaagctc cggtttaccc atattggaag aaggggacga gggtcgttac
     2101 ctcgtttcac tggattcaaa tgaacgcttt actttcaggg tgccttcact aaggaattta
     2161 aaattcacat tcccttatat gcatgacggc cggttctata ccgttgatga agtgctggat
     2221 cattacgcgt ccggtatcag ccatgctacc cggcctgacc cggcatttaa attaccggat
     2281 ggcagctatg gaatccctgt ttcacccgat caacgcaagg ctttaaaagc attcttactg
     2341 acactgaatg atacagcatt tgtaaaaaac cccctgtatg atgaggccgg gtctcaattg
     2401 tatattaaat aagctttcat ttctgaatgg aagagaaaga aggctgaccc gtaaaaatca
     2461 tcaggtttta ctgatcaaaa ggctactgca gtttctcatt gttcttatgc cggtccgcat
     2521 cccggactga tttcttttca aaattctttt ccaatgcctg tgtcaggtcc acgccggtct
     2581 ggttagccag gcagatcagc acccataata catccgccat ctcgtcgcca aggtcgcggt
     2641 tcttatccga ttctttaaaa ctctgatccc cgtaggtccg ggccataagc cgggccagct
     2701 cccccacttc ttcggtcaga atggtcatat tggtgagttc actgaagtac cggaccccgg
     2761 ttgttttgat ccagttgtcc acttgttgct gggcttcttg aatggtcata acaaaatatt
     2821 tgcaggaaaa ctgcaatata gcatgattct tgccgtttaa ctcttcccac cggctttccc
     2881 ggtggcccta tccgcccgat tcgttgcccc ggcaacggaa taccccttga aaacggcaaa
     2941 ccaattcctt taaaagatta atgtatccat cccatgtgga agtccattca acggctactg
     3001 gccttactgt cgctgtccct tttcatcaac gccgctgctg cgcagccggc cactaattca
     3061 tttgaccgct cctttaaaaa ggaaatcaag ccctttaaga tcctgacatc cggcaaacag
     3121 attaccataa aaagcaccag agatatcaag accatcatgg tctggacggc caccggccac
     3181 cggatcgtgg aacaaaccct ggtcaatgcc tccagctata ccttcaatgt atccgggtcc
     3241 cgcgaacgta ttttcttcgt catggtacaa tatgaaggac agaagccagt gacggaaaag
     3301 tttggggtgg aataatcagt aatcagtaat gagtaatgag taatgagtaa aagcagaaat
     3361 aactttacaa atttgaaact gttcctttag ggcaaacact tttcccgagc tactcattac
     3421 tgattattca ttactcattc ataagcagac tattactata taatagacct cttcccgagc
     3481 tactcattac tcattactga ttactcattc tttcgccttc gtatctatca ctatcgtcac
     3541 cggtccatca ttcagcagtt ccactttcat atccgctcca aatactccgg ttccaatggg
     3601 tttgcccagg tcggcggtca gttgtgtaat tattttttcg tagagtggta tggctgtttc
     3661 cggtttgctg gctttaatat aagagggccg gttgcctttt ttggtagcgg catgaagggt
     3721 aaactggctg accaggatga tatccccatc ccggtctttg accgattcat tcatcacccc
     3781 tccggcgtca tcaaaaatgc gcaggttcac gattttgctg ctgagccatt gaatatcctc
     3841 ttccgtatca gcatcttcaa ttcccagtaa taccaatagt ccccacccga tctgagcatg
     3901 gacttttccc ccgatggtta cgctggccct ggatacccgt tgtatgaccg cccgcatgtt
     3961 ttaaagattt tgttgatcac gaaaatattg aacattgaga attgagcatt gaataatgaa
     4021 cattttattc ctggctgtta ctgccgactg ccatctgccg cctgctgact ccaattggca
     4081 cgttggcata ttggcgcatt agtatttata tataatttta cgaaatgttt cactctcttc
     4141 ttcccgaaat cagtttccaa accacccgct ccggcggaaa aggcggacag aacgttaata
     4201 aagtggagac ggcggtaatc gtggctttcc atattaactc gtcggcttta ttgtcggggg
     4261 agcagaagca gctattgatg gaaaagttat cgaaccggat caacagtgaa ggctacctgc
     4321 aggtgaaggc ccagactcat cgtacccaac tggccaataa agaggaagct gtaaagaaaa
     4381 tacacgagct cgtaagcaaa gcactggaaa aaaagaaggc ccgcattgcc accaaagtgg
     4441 gcaaagcagc taaagaacgg agactggagt ctaaaaaacg aaaggcggat gtcaaatccg
     4501 ggcgtcaaaa gttcagaccc ggggattttt aagctgaacc gaaaaggcta tcttactgtt
     4561 tatatactgc agtgcgccgt aaactcatta ccatatcaac cctgatcctt tttgtggccg
     4621 gcttttcggt tattccggtg gcctgcaaaa aagagcaaag cacccgggga ctggaaaaac
     4681 tggcactgga aataccggat tcttttcccg acccggctta tgcatttaca gacaatccat
     4741 taagtaaaga agggtttgaa ctcggacgcc gcctttttta tgatccacaa ctctctgctg
     4801 acggactcgt tccctgttcc tcctgtcatc aacagatagc tggctttggt acgtttgaac
     4861 atgaccgcag ccatggcgta tttaattccc ataccctgcg caatgccccg gtattgttta
     4921 acctggcctg gaatacttct tttcactggg acggggaatt tacctcgttg gcggatgaag
     4981 ccgcccaccc gctgaccggc tccaacgaga tgggccagac ctaccaacgt attattgatt
     5041 atctggaagc tgaccgcact taccgggatg cctttaaaga agttttccgc tatcccttta
     5101 tccggcctga atttataaac aaagctttgg cccagtttac cggatccatt gtatcggcca
     5161 actcgaaatt cgacaaatac aaaaaaggaa cagttaactt ctccgcacag gaagaaagcg
     5221 gttatcaact gtacaaagtc aattgtgcca gctgtcaccc cgaacccctg tttacggatt
     5281 acagctaccg caatattggt ctgcggatcg atcctttgct gaatgattat ggccggttac
     5341 gcgttaccgg taaggccgaa gattcgctga aatttaaagt tcccaccctg agaaacacct
     5401 atatttcttc caattatatg catgacggaa ggttcaatac cttgctgcag tgcctgaatc
     5461 actaccggaa taatgttcag cagggtacca ccctcgaccc tttattagtc aatggaatta
     5521 gtttgtctga tacacagacc gacaaccttt tgctgttttt gcgggccctg accgattcca
     5581 gcctgctgcg agatccgcgt tttactaagc cggattagag tttattattt accctccggc
     5641 ctctcagtgc ttcgagcgcc tgtttttttg actctatctc tttttcagta tcgcgctgat
     5701 cctcctggtt gtttttgagt ttcttttcca gatcggactg atcatctttt aaaccacgga
     5761 gtttcttttc ggctttattg acagcctctt cctgggcaat gatatccagc tccaggttct
     5821 cggcttccac atcgggcagc agactgttga gaaaggcttt tacccggtca acctggtagg
     5881 catcaaagga ggttttcata ttgttgcctt ctttcataat gatcaggtac acgacggaag
     5941 catccgattt cttgcctttg gactccactt ttataatata gtccatttta tccgcgctga
     6001 tctcagtgat aaacgcatct ttgaaattac ggtagccctt gtctttattg aataaccctt
     6061 tttcctcttt tgacttatac ccgagcttgc tcattttttt gatcacggca ttttccaccg
     6121 cttccggggg ataaggatag tcaatcataa acacctgttg tttcttcttg tcataatcgg
     6181 ctgtgccttc ataagcttgt gcagaaagga ccaggttgag ccctgaaatc atcaataagg
     6241 ataaaataag ttgcttcata accatagaat ttacggataa agatacgtac cggggcccaa
     6301 gcgttgcaaa ccccttaaat aaaaaatcag tctggcatgg aattcgtttt ttgccccgct
     6361 aagaactact tttgtccggg ccggccccga caagccggcc ccttaaattt ccaatccatt
     6421 gagtggtatt aaaaaactgg ccggacaaac cctttggtat ggtgtaccca ccattgccag
     6481 ccgttttttg gggtacctga tgaacctgac cctgccactc atctttgcac aaccttcgaa
     6541 aacggcagac ctcacccagg tttatgccat tattccattt ctgaacgtcc tttttaccta
     6601 tggactggag acggcgtatt tccggttttc acaggaacag gatcagaaaa aactatataa
     6661 taccctgtct ctttcgctgt tcgggtccac gattcttttt accggggcac ttttcctttt
     6721 taaggatact attgcctcct gggccagcct tgaaaatcat cctgaatata taaactggat
     6781 ggccggaatt attttctttg atacaatcag taccctagct tttgcccggc ttcgccagga
     6841 aaataggcct aaacgatatg catttgcccg gttggccagt gtgttgatga atatcctggt
     6901 agtggtttta tttctcggca ttgttccccg gatggccgct gacagtcccg acagtttctg
     6961 gggagtactg catagtgata aagacaccgg cattgtttac tacctgattg gtaatttctg
     7021 tggcagtgta ctcactttcc tgatcctgca gaaagaacta aggcaggtac attttcagtt
     7081 tgacaacgcc ctctggaaaa aaataatgca gtattcctat cccctggtga ttgtaggcat
     7141 gggcggaatt gtaaacgata tgctcagccg gctgatatat cagcatgtgg tggatcttcc
     7201 tgctgaacag gccaaacacg aactcggtgt atttgccaat atcatccgtt tatccatcct
     7261 gatcaccatc gccattcagg cgttccggat ggcggcagaa cccttcttct tcaaccgcag
     7321 caaggaagaa aacgcgccga agacttatgc cagggtgatg aaattctttg tcctggtctg
     7381 ctgtttcctt ttcctcggca tcagcctgta tatcgatatc gtaaaatggt ttttcctggc
     7441 cattgaccgg tcggaatgga ccgaaggact gtatgtggta cccctgtttg ctatggccaa
     7501 tatttttctg gggatatatt ataacctcag tatttggtac aaactgacca ataagaatat
     7561 gatgggggct gtgatcaccc tggccggtgc tgtcatcacg atcgcactga acatcctgct
     7621 gatccccaaa ctccactatc tgggtgcggc gatcgccacc ttctgctgct acctgttcat
     7681 gatgatcatc agctatacac agggacaaaa acattaccct gtcccctatg cgaaaaagaa
     7741 actgattgcc tatctcgtat tggtaacctt actggtgctc acgcaccgcg gctttgtgta
     7801 tttcgttgac aaccgctggg ccaacctggc catagctacc ctcttactgg gcatgttcac
     7861 ctggttcgtc ggaaaaattg aacgaaaaga actggaaaaa ctcccggtaa ttggtaagtt
     7921 ttaccgaaag tagactctct tagcaggggc tgtttcctga cacccgacac ctgacacccg
     7981 ataccttttc ttagcaaggg ctattcccca acacctaaca gctaacacct aacagcttaa
     8041 tgtaagggct gtttcctgac aaccgacatc agacaactga caacttccca cagcaggggc
     8101 tgttccccaa cacctaacag cttttacctg acaaacggat tattcccccg ctcatacccc
     8161 accgtagtca ccggcccgtg cccggaatac actttggtat cgtccggcag cgtaaaaaat
     8221 tgtgtccgga tactattcat taaggtttcc atactgccac ccggaaggtc ggtacggccg
     8281 atactgcggt taaacaggac atctccgccg ataataaaac cattttccgc actgtaaaag
     8341 gaaatactgc cgggtgaatg accgggggta aaccggattt ccagctcatc ctccccgatt
     8401 ttaataaaat ttccttcttt caaataaacc agggggccct cataattgtc aaagggaagt
     8461 tgccagcgct cgcctgatac tggcgcaaag tccaggacca gcttttcctt ttcatggata
     8521 tgcaggtcca gtccccaggt atcatgtacg aatttgttcc cgaaaacatg gtccaggtga
     8581 caatgggtgt tcaacagtaa aaccggggtg agcccggctt tttcaatgcc cgtttttaat
     8641 tcgtcctttt cagccggaaa ataacagccc gggtcaataa tgcagcattg tttttgttcg
     8701 ttatacaaaa cataggtatt ctcctggacc ggactaaagg tgaaagactt aacaactaac
     8761 atggatggtt ttttcgtact tttaaagaca atcccggaaa gccggtttcc ggaagacctg
     8821 atacggggtc aaaagtacaa aatagctact tttgaaccgg gatcccggcc aataactgct
     8881 gaaccggcat tcgatgttcc tgccaacgcc tcatcattat aaaaggcagg ctgccaaaga
     8941 agcgatttaa acgatttaag aattttgaac agtaagtcat agtctatgaa gaaagtcctt
     9001 agccttattg cctgcct
//
LOCUS       DAOPMW010000103         8999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8516_length_8999_cov_7.961091, whole genome shotgun sequence.
ACCESSION   DAOPMW010000103 DAOPMW010000000
VERSION     DAOPMW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8516_length_8999_cov_7.961091"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PLU11_06745"
     CDS             complement(<1..1179)
                     /locus_tag="PLU11_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58851.1"
                     /translation="MKRVYFLLVFLMASGSIFSQLLTENFEYTAGQALTANTWTAHSG
                     AGTNAISVVSPGLTYSGHPGSGVGNAASMINTGEDVNKTFSGPVTSGSVYASFLVNFS
                     AIQTGDYFTGFLQTTSIFPVRVYAKTDGAGGFFFGVSKGSSAAVYETNARALNTTYLV
                     VIKYTFNTGSTTDDEINLWVNPALGGTETTATIAGVTSTGGDATTIGAIMLRQGSATN
                     APSQKVDAILAGTTWESVTPSATVPALSATSLNTFGSVCINTTAGPNSFDINGSNLTT
                     ADVTIGALDGFTYSATAGGTYTSSLSITQPGGTFAQTVFVKFDPTLVQTYNGSIPVGG
                     GGVSSTVNVAASGSGINNAPVVTSGTATAITQISATVSGSITDAGCSPITSYGIEYST
                     T"
     gene            complement(1233..2744)
                     /locus_tag="PLU11_06750"
     CDS             complement(1233..2744)
                     /locus_tag="PLU11_06750"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPN58852.1"
                     /translation="MQNKFNHKWIIPALGLLMAGLLMISCKRTFTDPPELGAPDMVAN
                     ISIKDIKARYTSGAPVAITDDAVIEGVVSCDDRSGNYYQQIAIQDSTGGVLLRLAGNN
                     HFNNYPVGRKLFVKLKGLYLGQYNGTLQFGGGVDTPYLNQGGVTLLATNLQNLHIIKG
                     ALNQPLVPKLVTVSQLTTSLQDRYVSTLIKLSGYEFSAADRSKNYADDGFSGNRIIQD
                     CSNLNANKITLRTSDFSNFATLPVAQGNGDIIAVYSFFGSTKQLTIRDTTDVRFNNPR
                     CPTFSGEGQVTLGSSSPFIINFDNLGTVGLPAGVYVKQDATAGFVGNEGSVYNTNFLT
                     TTAWNQTSAGFKNYASATGLTATATSTVQNASTNRALGARQTGSLGDPGFAFAFQLNN
                     TTGKTNLKLEFLLQSLDGSGSTAGRTTSWKVDYGVGDNPVVFTQVVSTPAPLNTVYGT
                     PGNFTSTPVTVDFGAALNNLNQKVWIRIVTLNGTTGGGNRPSSAIDDVKFTWN"
     gene            complement(2774..3484)
                     /locus_tag="PLU11_06755"
     CDS             complement(2774..3484)
                     /locus_tag="PLU11_06755"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPN58853.1"
                     /translation="MSFTQIKYTLTALIFSVLFVSCDKMPIGPTGPDPLGPGDYMSFS
                     EFRALYTGSGDYTIPTGTKRIRGVVISNNTNEASGVYRLQDESGAGIYLYTVIGSPVY
                     PMGSVLDINATGGGKLTLYNGDLELMSIPQSNISQVSGAITVTPRVATVAEIIANKNT
                     WASSLVKINNVTSITQSSSNSTGITYNITDATGTLSMFVRTASGITVNTSGTSVTGYI
                     SIYLTSTQIGIRTAGDIQ"
     gene            complement(3517..6108)
                     /locus_tag="PLU11_06760"
     CDS             complement(3517..6108)
                     /locus_tag="PLU11_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58854.1"
                     /translation="MLKNKLLLLLCCFSLAAGAQVDSTAKKDTVIIDELKDGVLDNIP
                     TVSLDENDMGDGGNQNISSLLTAGRDPFYSAASFNFSAVRFRIRGYDNDLFSTYMNGI
                     PMDNLDNGFTPYGLWGGLNDVMRNRDVSWGLRYNTFAFGDIGSNTNIDSRASKQRAQT
                     SISYAASNRNYNNRIMLTHSTGVSKKGWAFSLSGSRRWADEGYVPGTYYNGWGYFAAV
                     DKRIGQRHLLSFTTFGAPTENGRQGAAVQEMMDLAGTNYYNPNWGYQNGEKRNASIGK
                     THQPVFILTHDFRLNNKTTLTTAAGYSLGNRSVTALDWYNAADPRPDYYRYLPSYTST
                     WSSSTDPSQGQQLYDLMKNDINYRQINWQNLYNVNRASTATINNANGITGNSVTGLRS
                     RYIIEERIINTKRFNANSVLNTKFGEHIDFTAGASYQQQNNHYYKKVDDLLGGQFYVN
                     VNQFAERDFPVDNLANQFDLDQPNRILYKGDKFGYDYNINISRAAGWAQGVFRYNKVD
                     LFLAGNLSYTKFYREGNVRNGLFPNDSEGKGTINEFTDYAVKAGVTYKIDGRNYLFVN
                     GSYQTRAPYFENVFISPRTRNAQQEDVTSETIQTVEGGYVMNAPKFKIRLSGYYTRFQ
                     DGVDVMSFYHDTYQNFVNYAINGIDKLHFGGEFGIEARLTPTITLNAAAAVGRYYYDS
                     KQTAVITVDNSASVLGRQLIYAENFRLPSTPMEAYSFAFNYRSPKFWFISLTGNYFDK
                     SYLSMNPLRRTWDALQYVAPKSEAYNAIFDQTQFDAQYTVDFFGGYSWKLPKDFEVNK
                     KSTFLVFNVGVNNILNNKDIVTGGFEQLRYDAQLSSTDPVQVGKFPPKLFYAYGLNFF
                     ASMTLRF"
     gene            6266..7324
                     /locus_tag="PLU11_06765"
     CDS             6266..7324
                     /locus_tag="PLU11_06765"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HPN58855.1"
                     /translation="MSRITIVFFLLISCLTLPAQKKDYKSAVIAFYNLENLYDTVDNT
                     LTNDEEFLPKGDRNYGSAIYNNKLNKLATVISQIGTELNPEGPALLGVAEVENDTVLN
                     DLVRQPAIAKRNYRIVHYDSRDIRGVDVGLLYNPKYFVVEASDKLFVQLPGGSKDAYF
                     TRDILWVKGKLDGETVHIYVNHWPSRSGGEVRSAPARNAAAQVCRNHMDSITKKEPNA
                     KIIVMGDLNDDPINESVSGIINAKGKEKDVKPGGMFNPWMDMYKKGIGTLAYQDAWGL
                     FDQILISQAWLKKEQSGFFYYQPHIFNKEYLVENQGRYKGYPMRTWDGNTYRGGYSDH
                     FPTYIFFLKEIKPVKKGF"
     gene            7534..7890
                     /gene="rpsF"
                     /locus_tag="PLU11_06770"
     CDS             7534..7890
                     /gene="rpsF"
                     /locus_tag="PLU11_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPN58856.1"
                     /translation="MNNYELMVIFTPVLSDDEFKAEQKKYAKLVTDAGGSIVHENPWG
                     LKSLAYPIQKKTTGLYWVMEYTAQSDFNEKLKIQLLRDESVLRHLCTKLDKYAVEYNA
                     KKKSGVKTGTEKAVEA"
     gene            7895..8158
                     /gene="rpsR"
                     /locus_tag="PLU11_06775"
     CDS             7895..8158
                     /gene="rpsR"
                     /locus_tag="PLU11_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPN58857.1"
                     /translation="MAKNEIKYLTAIKSDKRVKKFCRFKKYGIRYIDYKDVEFLKKFL
                     NEQGKLLPRRLSGNSLKYQRKVSDAVKKARQMALLPYVTDLLK"
     gene            8232..8678
                     /gene="rplI"
                     /locus_tag="PLU11_06780"
     CDS             8232..8678
                     /gene="rplI"
                     /locus_tag="PLU11_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPN58858.1"
                     /translation="MEVILIQDVDNLGGINEVVKVKNGYARNYLLPRKLAVENSPSNK
                     KQLEERMKQVRKKEDKMLAEINSVIAKLNEAPIKLGAKTGTSGKIFGSITTLQLSRAI
                     RDQRGYDIDRKKMTIPEDVKELGSYKATIDFGNGKSTEIDIEVVAE"
BASE COUNT         2534 a         2012 c         2095 g         2358 t
ORIGIN      
        1 tgtggtacta tactcaatac cataactggt aatgggagaa cagcctgcat cagttatgga
       61 acctgaaacg gtagccgaaa tctgtgtaat ggccgtagcc gttccactgg taacaactgg
      121 cgcattattt ataccgctac ccgaagcagc tacattcacg gttgagctta caccaccacc
      181 tccaacaggg atagatccat tataggtttg caccagcgtg ggatcaaatt tcacaaatac
      241 agtctgcgca aacgttccgc ccggctgtgt aatagagagt gatgaggtat aagtgccacc
      301 cgcagtggcg gaataagtaa atccgtcaag tgcgccaatg gtaacatcgg cggtggtaag
      361 gttggaaccg ttgatatcaa aactgttcgg tccggcagtg gtgttaatac aaacgctgcc
      421 aaatgtgttt aatgaagtgg ctgaaagtgc cggaacagtg gcggatgggg tcaccgattc
      481 ccaggttgtg ccggcaagaa ttgcatccac tttctgggat ggtgcatttg tagcggaacc
      541 ctgccgcaac ataattgcac caatcgttgt tgcatcaccg ccggtactgg ttacaccagc
      601 aatggttgcg gttgtttcag tccctcccag cgccggatta acccacagat taatttcatc
      661 atccgttgta cttcctgtat taaatgtata cttgatcacg accaggtatg ttgtattcag
      721 tgcccgtgcg tttgtttcat acaccgcagc agaactgcct ttacttaccc caaagaaaaa
      781 accaccagca ccatctgttt tggcataaac gcgaaccgga aaaattgagg tggtttgcag
      841 gaatccggta aaatagtcgc ctgtttgaat agccgaaaaa tttaccagaa atgaagcata
      901 aacacttccg gatgttacag gaccggagaa agtcttattt acatcttcac cggtattgat
      961 catggaagct gcattgccga cacccgaccc cggatgcccg gaataggtaa gccccggaga
     1021 aacaactgaa atcgcattgg tacctgcccc agagtgagct gtccaggtat tggcggttag
     1081 tgcctgaccc gcggtatact caaaattctc tgtaagcaac tgactgaaaa tactgccaga
     1141 agccatcaga aaaaccaaaa gaaaataaac tcgtttcatt actgtcaatt tatatttttt
     1201 cagccaaaga ctgaatggtg taaagtatgc tgttagttcc acgtaaattt cacatcatcg
     1261 atagcactgg aaggacggtt accaccgccg gttgttccgt tcaatgttac aatccgtatc
     1321 cagactttct gattcaggtt attcagggcg gcaccaaaat caaccgttac aggggtactg
     1381 gtaaaattac ccggtgtacc ataaacagtg tttagcggtg ccggagttga aacaacctgt
     1441 gtaaatacta ccggattatc ccccaccccg taatccactt tccaggaagt ggttcgtccg
     1501 gctgttgaac cggatccgtc gagcgactgt agcagaaact ccagtttcag gttggttttt
     1561 ccggtggtat tattcaattg aaaagcaaat gcaaaaccag ggtctcctaa tgaaccggtc
     1621 tgtcttgccc ctaaagcccg gttagttgag gcattttgaa ctgtacttgt ggcagtagca
     1681 gttaatccgg tagccgatgc atagttttta aagcccgcac tggtttgatt ccaggccgta
     1741 gttgtcagga agttggtgtt atatacggat ccttcatttc caacaaaacc agccgtggca
     1801 tcctgcttta cataaacccc tgcaggaaga ccaaccgtac ccaggttatc aaaattgatt
     1861 ataaagggcg agctgctgcc taacgtgacc tggccttcac cactgaaagt ggggcaacgc
     1921 ggattattga aacgtacatc cgtcgtatcg cggattgtca gctgtttggt ggatccgaag
     1981 aaggagtaaa ccgcaatgat gtccccattg ccctgcgcaa caggaagtgt ggcaaaattg
     2041 ctgaaatcac tggtgcggag agtgatttta ttggcattca gattggaaca gtcctgtatg
     2101 atccggttac cggaaaaacc atcatccgca taatttttgg aacgatccgc ggctgaaaat
     2161 tcgtaaccgg aaagtttaat caatgtactt acatagcggt cctgaagaga ggtggtaagc
     2221 tgactgacgg ttaccagttt tggcaccagg ggttgattca gcgctccctt tatgatatgc
     2281 agattctgca gattggtggc cagcagcgta accccgccct ggttgagata gggagtatcg
     2341 acaccgccgc caaactgaag ggtaccatta tattgcccga ggtaaagtcc cttaagttta
     2401 acaaacaact tgcggcccac cgggtaatta ttaaaatgat tgtttccggc caggcgcagt
     2461 aatactccac ccgtagaatc ctggatggcg atctgctgat aatagttacc gctgcggtca
     2521 tcacaggaaa ccacaccctc tatcaccgca tcatcggtga tggctaccgg tgcacctgag
     2581 gtataccggg cttttatatc cttgatactg atattcgcca ccatatccgg cgctcccagt
     2641 tcaggcggat cagtaaatgt tcgcttacag gaaatcatca acagtccggc catcagtaaa
     2701 cccagggccg ggatgatcca tttatgattg aatttgtttt gcataattta tttgttgtgc
     2761 agtcagttaa tatttattgg atatcccctg cggtacggat accgatctgg gtactggtta
     2821 aataaatgga aatgtaaccg gtgaccgaag tcccgctggt atttaccgtt atgccggaag
     2881 cggtcctgac aaacatggac agcgtacctg tggcatcggt gatattatag gtaatccccg
     2941 ttgaattgga tgaggattgc gtaatagagg tcacattatt aatcttcacc agcgaagaag
     3001 cccaggtgtt tttgttggca ataatctctg ctacggtggc cactcttggt gtaaccgtaa
     3061 tagccccgct tacttgtgaa atattggatt ggggaatact catcagttcc aggtcgccgt
     3121 tgtataacgt cagtttacca ccgccggtgg cattgatatc gagaacagaa cccatgggat
     3181 ataccggcga gccgataacc gtataaagat aaatacccgc accgctctcg tcctgcaggc
     3241 ggtatacgcc ggaagcttca ttggtattgt tggagatcac cacaccccgg attcttttgg
     3301 ttccggttgg aatggtataa tctccgctcc cggtatataa ggcccggaat tccgaaaagc
     3361 tcatataatc gccggggcca aggggatccg gtcctgtagg gccaataggc atcttatcac
     3421 aggagacaaa cagcactgaa aagatcaggg cggtcagtgt atattttatt tgtgtaaaac
     3481 tcatactaga ttttattagc agttaatgtc tttaaattaa aaacgcaggg tcatgctcgc
     3541 gaaaaagttc aggccatacg catagaacaa cttcggagga aacttgccca cttgtacggg
     3601 atcggtggat gacaattgcg catcataccg cagctgttca aatccgccgg tgacgatatc
     3661 cttattattc aggatattat tgactcccac attaaataca aggaaagtgg attttttatt
     3721 gacttcaaaa tctttgggca acttccagga atagccacca aagaaatcca cggtgtattg
     3781 cgcatcaaac tgtgtctggt caaaaatagc attatacgcc tcacttttag gagccacata
     3841 ctgcagggca tcccaggtgc ggcgcagcgg gttcatactg agataggatt tatcaaaata
     3901 attaccggtc aggctaatga accagaattt aggagaacgg taattgaacg caaagctgta
     3961 ggcctccatg ggtgtggagg gtaaccggaa gttctcggca tagatcagct ggcggcccag
     4021 cacagaggca ctgttatcca cggtgatcac cgctgtctgc ttgctgtcat aataataacg
     4081 gccaacggca gccgctgcat tcagggtaat agtcggcgtc agcctggctt ctataccaaa
     4141 ttcaccacca aaatgaagtt tatcaattcc attaatcgca tagttgacga agttctgata
     4201 ggtatcatgg tagaaggaca ttacatccac accatcctgg aatcgggtgt aatacccact
     4261 taagcgtatt ttgaattttg gcgcattcat gacataacca ccttccaccg tctgaatggt
     4321 ttcactggtt acatcttcct gctgggcatt gcgggtacgt ggagaaataa acacattctc
     4381 aaaatacggg gcccgggtct gataagaacc gttaacaaat aggtagttac ggccatcgat
     4441 tttataagtc actccggctt ttacggcata atccgtaaac tcattgatcg ttcctttccc
     4501 ttctgagtcg ttagggaaaa ggccattgcg gacatttcct tcccggtaaa attttgtata
     4561 ggacaggttc ccggccagga acaaatccac tttattatag cggaaaacac cctgggccca
     4621 accggccgcc cgggaaatat tgatattata atcatacccg aacttatccc ctttataaag
     4681 aatgcggttg ggctgatcca ggtcaaactg gttggccagg ttatctaccg ggaaatccct
     4741 ttcagcaaac tggtttacgt tgacataaaa ctgaccaccg agtaaatcat ctactttttt
     4801 atagtagtga ttattctgtt gctggtaaga agcgccggcg gtgaaatcaa tatgttcgcc
     4861 gaatttggta tttaaaacgc tgttggcatt gaaccttttg gtattgatga ttctttcttc
     4921 aatgatgtaa cgggaacgaa gacccgtgac actgttaccg gtaatgccat tggcattatt
     4981 gatggtggct gtgctggccc ggttaacatt gtaaagattc tgccagttga tctgccggta
     5041 gttgatatcg ttcttcatca ggtcatacaa ttgttgtccc tgtgaaggat cggtggaact
     5101 ggaccaggta ctggtatagc tgggcaggta acggtaataa tccgggcgag gatccgcggc
     5161 attgtaccag tccagcgcgg taacactgcg gttccctaat gaataaccgg cggctgtggt
     5221 cagggtggtt ttattattca gcctgaaatc atgggtaagg ataaataccg gttggtgggt
     5281 ttttccaata ctggcatttc ttttttcccc gttctggtaa ccccagttcg gattataata
     5341 attggtgcct gcgaggtcca tcatttcctg aacagccgct ccctggcgac cgttttcagt
     5401 gggtgcaccg aaagtggtga aggaaagcag gtggcgctgt ccgatccgct tatccacagc
     5461 ggcgaaatag ccccagccat tataataagt accgggaaca tacccttcat cggcccaacg
     5521 gcgactgcca ctcagtgaaa acgcccatcc ttttttactg acaccggttg aatgcgtgag
     5581 catgatccgg ttattgtagt tacggttgga cgctgcatag ctgatactgg tttgtgcccg
     5641 ctgtttgctg gcgcgggaat cgatattggt attgctgccg atatcgccaa aagcaaatgt
     5701 attatacctt aagccccagg atacatcccg gttacgcatt acatcattca atccgcccca
     5761 gagaccatat ggggtaaaac cattatccag gttatccatc ggaatcccat tcatgtacgt
     5821 actgaagaga tcattgtcat acccgcggat acggaaacgt accgcactga agttgaaaga
     5881 ggcggcggaa taaaacggat ctctgcccgc agtcagcagc gaggaaatat tctgattacc
     5941 accgtcgccc atatcgtttt catccaggga aacggttgga atattatcca gtacaccgtc
     6001 tttaagctca tcaatgatta ctgtgtcttt tttagctgtg gagtccacct gtgctccggc
     6061 tgcgagagaa aagcagcaga gcagcagcag cagtttgttt tttagcatgt cagtttattt
     6121 aaacgagagt gcaaaattac taaggatatc ctacccattt taaaaaaatt aggcacatca
     6181 taataaagta atacacctgc gcagctaatt tctatggttt gtcgtccaat gattctgtat
     6241 gtttgtgccc ctttatacga attttatgag cagaatcacc atcgtatttt tcctgttgat
     6301 aagttgcctt accttacccg cacagaaaaa agactataaa agcgccgtta ttgccttcta
     6361 caacctcgaa aatctttacg atacagtaga taacaccctt accaatgatg aggaatttct
     6421 gcccaaaggg gaccgcaact atggttctgc tatttataat aataaactga ataaacttgc
     6481 cacggtcatt tcccagattg ggaccgagct caaccccgaa gggccagcgc ttttgggggt
     6541 ggctgaagtg gagaatgata cggtgctgaa tgacctggta cgtcaacccg cgattgccaa
     6601 aaggaattac cggatcgtac attatgattc cagggatatc cggggtgtgg atgtcggtct
     6661 tttgtataat ccgaaatact ttgtggtgga agccagtgac aaattatttg tgcaactgcc
     6721 gggcggatca aaagacgcgt attttacccg ggatatcctt tgggtaaaag ggaagctgga
     6781 cggggaaacg gtacatatat atgttaatca ctggccgagc cgtagtggtg gtgaagtgcg
     6841 gagtgccccg gcccgtaatg ccgcggcgca ggtttgccgc aaccatatgg actcgatcac
     6901 caaaaaagaa cctaatgcca agatcattgt catgggtgac ctcaatgacg atcccatcaa
     6961 tgagagcgta tccggtatca ttaatgccaa aggaaaggag aaggatgtaa agccgggcgg
     7021 catgttcaat ccgtggatgg atatgtataa aaaagggata ggaacgctgg cctaccagga
     7081 tgcctggggg ctgtttgacc agattcttat ttcacaggcc tggctgaaaa aggaacaaag
     7141 cggtttcttc tattaccagc ctcatatttt taacaaggaa tacctggtag aaaaccaggg
     7201 ccggtataaa ggctatccca tgcgcacctg ggatgggaat acctaccggg gcggatacag
     7261 cgaccacttc ccgacttata tctttttcct gaaagagatt aaacctgtta aaaagggatt
     7321 ttaattttaa gtagctgaat ttcaacgtat tgaatttcat atattgaaag agttgcgggc
     7381 ttcagataca actccttttt ctgtaaggaa tctgcccgga aacccctatc tttgccgccc
     7441 caaatctgta ttcgcagatt ctgtcttttg cagctccggc gaatgaagac tgtccaatgg
     7501 gtaaaccgaa ttattaaatc aaaaaaaggt aaaatgaaca attacgaatt gatggtgatt
     7561 tttacccccg ttttaagtga cgatgagttc aaagctgagc agaaaaaata tgcgaagctg
     7621 gtaaccgatg ccggtggctc tatcgttcat gaaaatccct ggggactgaa atcacttgcg
     7681 tatccgatcc agaaaaagac aaccggtctg tactgggtta tggagtacac cgcgcaatcc
     7741 gacttcaatg agaagctgaa aatccagctt ctgcgtgacg aaagcgtgct tcgtcactta
     7801 tgcactaaac tcgataaata cgccgtcgag tacaatgcca aaaagaaaag tggtgtaaaa
     7861 actggaaccg aaaaagcagt ggaggcttaa tacaatggca aagaatgaaa ttaaatacct
     7921 gaccgccatt aaaagcgaca aacgtgtaaa gaagttttgc cgctttaaga aatatggcat
     7981 ccgctacatc gattataaag atgtggagtt cctgaaaaaa ttcctgaacg agcagggtaa
     8041 attattaccc cgtcgcctct ccggtaacag cctgaaatat cagcgtaaag tttccgatgc
     8101 agtgaaaaaa gcccgtcaga tggccttatt gccttacgta acagatcttt taaaataacc
     8161 tcaccctaac cccgtaaaac gggagacagt tttcgtaaat ggaaactggg ttggtgaaca
     8221 aaaagaaaac aatggaagta atcttaattc aagacgtaga taatctgggt ggcatcaacg
     8281 aggtggtaaa agtaaagaac gggtatgccc gtaactattt actgccccgt aagctggcgg
     8341 ttgaaaacag ccccagcaac aagaagcaac tggaagagcg tatgaagcag gtgcgcaaga
     8401 aagaagataa aatgctggct gaaatcaaca gtgttatcgc taaactgaat gaagccccga
     8461 tcaaactggg tgctaaaaca ggtaccagcg gtaaaatctt cggaagcatc accacgctgc
     8521 agctcagccg cgctatccgc gatcaaagag ggtatgatat cgatcgcaag aagatgacga
     8581 tccctgaaga tgtaaaagaa ctgggttctt acaaagctac catcgacttc ggtaatggta
     8641 aatccactga gattgacatt gaagtagtcg ctgaataatt tcccccactt ttttataaaa
     8701 aacctcccat ccgggaggtt tttttgttta aataatacaa gtatttgact atcagtattt
     8761 taaatcatta agcatctctt tacagaaaaa ttattactat ctcaaaaaaa tttccgttat
     8821 tttgtcttgc ttttggtgct ctttcagttt tacaagtcct gaacgattcg aaaaaccata
     8881 gaatgttcat tgggtactgt tactgttgac acttttatat ttaaaatttt caaaatgaac
     8941 atttacgtag gaaatctcag ttggaacctg aaggatcagg atttatctaa cctttttgc
//
LOCUS       DAOPMW010000104         8963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8565_length_8963_cov_6.691850, whole genome shotgun sequence.
ACCESSION   DAOPMW010000104 DAOPMW010000000
VERSION     DAOPMW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_8963_cov_6.691850"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLU11_06785"
     CDS             complement(<1..466)
                     /locus_tag="PLU11_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN58859.1"
                     /translation="MEFLKTLQIGTTNPGVSTGTRWITSAGETIDSYSPVDGKIIASV
                     TAADKAGYETVMQKAEEAFKTWRLVPAPRRGEIVRQIGDALRENKEPLGKLVSYEMGK
                     SLQEGYGEVQEMIDICDFAVGLSRQLHGLTMHSERPAHRMYEQYHPLGIVGII"
     gene            complement(483..2396)
                     /locus_tag="PLU11_06790"
     CDS             complement(483..2396)
                     /locus_tag="PLU11_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58860.1"
                     /translation="MKSMRPGALCLFLFISFSLHAQDSSLLLSNVIITATQTAQQQKE
                     SGRNIIAINGAAFNTLPVQSVDELLRYLPGIEVQQRGPQGAQADITIRGGTFQQVLVI
                     IDGVKLNDPLTGHFNLYIPLHPAEIERVEILKGAAAAVWGSEAVGGVVNIISKSFSGK
                     KYNNEYRGRIVAGEYGLLNTAVYGRWTAHKNSFSGGLFSNNTNGQPLRGTKGYFNLHT
                     ANLSFSRQLTNNWRVSLRTAADFRKFNAQNFYTSFASDTANEIVNSAWIQAALQKKYK
                     KGQLQIDAVYKKLRDQYWFRPTAVPNDNRAGFYASQAYFSGIINEKNGFTAGVQHSLK
                     FIKSNDRGEHSLWHGALYGILRHHPGRNLYINESLRLDWDESYGWVLVPQLNMAWSLS
                     KLTLRAAAGKSFRDADFTERYNNYNKSLVSSGRIGNPALKAERAWNMEFGADYNFSSR
                     FRISSGFFYRDHEDLIDWANTAYADMPRKDNLVPGGSYALAKNVEFIRTSGLELDIMY
                     NEKTGPNSQLLAMAGFTWLKSVNDDPVPSLYISSHARILLNYSIAWTVKRFSFSIAGL
                     YKERAEQFSAPLNSKLTPSYFLMNVKAGWQFIPVKGRLFLQADNLFNTAYIDLLGAQM
                     PGRWLSGGIEIAL"
     gene            complement(2477..3295)
                     /gene="panB"
                     /locus_tag="PLU11_06795"
     CDS             complement(2477..3295)
                     /gene="panB"
                     /locus_tag="PLU11_06795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPN58861.1"
                     /translation="MSSVKKEVKRVTTHTLQKMKAAGEKISMLTAYDYSFARIIDGAG
                     IDVLLVGDSASNVMAGHETTLPVTLDQMIYHASSVIRGIERCFVVVDLPFGSYQSNTE
                     IALASAIRIMKETGAHAVKLEGGEEVIDSVKKIVSAGIPVMGHLGLMPQSIYKFGTYE
                     VRAKEENEAGKLREDARLLEQAGCFALVLEKIPAELATSVSSSLSIPTIGIGAGNGCD
                     GQVLVMHDMLGINTEFKPRFLRQYLNLAEQATGAVQQYILDVKSGNFPNEQEQY"
     gene            complement(3321..4217)
                     /locus_tag="PLU11_06800"
     CDS             complement(3321..4217)
                     /locus_tag="PLU11_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPN58862.1"
                     /translation="MKLAAIDIGSNAARLLIMEVANGPQGTTEFIKTMLVRVPLRLGF
                     DVFDKGEISAGKAEKIIKTIKSYRLLLDVYEVKHLKACATSAMRDATNGPDIIKQVKA
                     ETGLDIEIISGDQEASLIYENHVAENMANDESYLYIDVGGGSTELTFFSDGKLIFKKS
                     FNIGTIRMLKNQVTESSWDELKDYIRSKTKGYHHVTAIGSGGNINKVFSLSKRKDGKP
                     LHLDLLRDYYKEFSNLTLFQRISLHKLREDRADVIVPALLIYINIMRWADAEEIYVPK
                     IGLADGLIQNLYEEIRLRELEV"
     gene            complement(4198..6291)
                     /gene="ppk1"
                     /locus_tag="PLU11_06805"
     CDS             complement(4198..6291)
                     /gene="ppk1"
                     /locus_tag="PLU11_06805"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPN58863.1"
                     /translation="MPENPKHKTIARDISWLSFNARVLQEAADNSVPLRDRIRFLGIF
                     SNNLDEFFRVRVATLKRMIQFGTKARMHLENNPQQIIDEIQMTVLNQQGEFNRIWEEC
                     LVELNKQKIFLRNEKELNPEQQEIVRTYYEEEVSADLIPLMIENIPVFPMLRDKSIFL
                     AVVMWKKESAFHKKYALIEIPSRIHGRFKILPSGNEEKHIILLEDVIRFNLPDIFSYF
                     GYDHYQSHIFKVTRDAEIDIDDDLSTNIIQKIEKGVKNRRKGKPVRFVYDREMDPGLL
                     EYLVRRLNLSKKDNLIPGGRIHNFRHFMDFPDEVFKEKRKRNKPFDHPLMTERRVSDL
                     IQEKDILLHFPYHSFTPLIDLLREAAFDPEVTTIKITCYRLASQSKIINALINAVRNG
                     KEVIVMIELRARFEEEANIEWKNRLEEEGARVLVEIPHMKVHSKLCLIRKRTKNNTIL
                     QYGFVSTGNLNEKTARIYADHCLLTSNPKIMGDVNRIFHFLEQPKTGLHFLKDCRYVL
                     PSPHFVKKEMIRLIEEEIKQARKKKPASIILKMNSLSDEILIAKLEEAAKAGVEIRLI
                     VRGIFGLYSENKKYQSPIRAISIVDEYLEHARVWVFHNKGKQKVYLSSADWMLRNLEH
                     RVEATCPVWHPELKKELIDILNIQLEDNVKARRLDNELSNDYVRTEKKKIRSQVETYK
                     FLHKKTLTQIETGSH"
     gene            complement(6376..6867)
                     /gene="rfaE2"
                     /locus_tag="PLU11_06810"
     CDS             complement(6376..6867)
                     /gene="rfaE2"
                     /locus_tag="PLU11_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPN58864.1"
                     /translation="MNRADLIPHKIISRELAVQQVTQWKTMGRKVAFTNGVFDILHPG
                     HIFSLSEAAKEGDYLVVGLNADASVKRLKGESRPVNNQDARAILLAALLMVDAVVIFE
                     EDTPLELITAILPDVIIKGGDYTPDQVAGGKEVIAAGGKVVINPILEGYSTTALIEKM
                     KGE"
     gene            complement(6864..7868)
                     /locus_tag="PLU11_06815"
     CDS             complement(6864..7868)
                     /locus_tag="PLU11_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPN58865.1"
                     /translation="MKKNIRTILQYLLFLGLGIFFAWLSLKNLDQEKTEQIKMAFRNA
                     RHWLAIPVFLILIGSHWVRAIRWRLLIEPLGYTPSRANSFFAVMIGYLVNMGVPRLGE
                     IVKCTVLARYEKIPADKLIGTIILERMIDALTLLLVFGTTLLLQPQLYSQIIEAFFSS
                     NEVQTEKKVSTLLVTLIIVAAIALLIGLWMLIRKKTIHDLLVLFRKVYLSVMQGISTI
                     RHLKKRGLFLVYTFLIWGFYLAGGYIGFMAFQETELYGVKEAFTILSAGSIGMIASPG
                     GIGAYAYLIEKTMQLYGLNYTIALAFGWILWLAQTTVILLGGILSFGALPYFNKKAKP
                     "
     gene            complement(7928..8779)
                     /gene="panC"
                     /locus_tag="PLU11_06820"
     CDS             complement(7928..8779)
                     /gene="panC"
                     /locus_tag="PLU11_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPN58866.1"
                     /translation="MILFKKAIDLRKHLDVQRKSGKKIGFVPTMGALHEGHLSLIQAS
                     KSATTLTVCSIFVNPTQFNDPADFEKYPLSTDKDIALLLSAGCDLLFLPDVDEMYPDG
                     LTSNQKFDLGEMETLLEGKFRPGHFQGVCQVVNQLLLQVEPDALFMGQKDYQQCMVIN
                     RLLQQTGNCEKIQLHICPTLREPDGLAMSSRNRRLNEADRVISVTIYRTLSFLKENLK
                     AGKLDVIQKSAFDQLQAAGFRTDYIVIADATTLQPVTYWDGKQQLVALVAAFLGEVRL
                     IDNLPLN"
BASE COUNT         2279 a         2153 c         2015 g         2516 t
ORIGIN      
        1 aaatgatacc cacaataccc agcggatgat attgttcata catccggtgt gcgggccgtt
       61 cactgtgcat ggtcagtccg tgcagctgcc gggaaagtcc aaccgcaaaa tcacagatat
      121 caatcatctc ctgcacttcc ccatatcctt cctgcaggct ttttcccatt tcataggaga
      181 ccagtttacc aaggggttct ttattttcac gcagggcatc accgatctgc cgtacaatct
      241 caccccgacg gggggcaggc actaatcgcc aggttttaaa cgcttcttct gctttttgca
      301 tcaccgtctc atagcccgct ttatctgccg ccgttaccga ggcgattatt ttaccatcaa
      361 caggagagta agagtcaatc gtttcaccag cggaagtgat ccaccgggtg ccggtactta
      421 caccagggtt ggtggtaccg atctgtaaag tcttcagaaa ttccatatgt aatatttaaa
      481 ggtcacaaag ctatctcaat tccgcctgat aaccatcggc cgggcatttg tgcgccgagt
      541 aaatcaatat aagcggtatt aaagagatta tccgcctgca aaaaaagccg gcccttcact
      601 ggaataaact gccagcctgc ttttacattc atcagaaaat aggagggggt gagtttgctg
      661 tttaaaggcg cagaaaactg ttcggctctt tctttatata acccggcaat agaaaaactg
      721 aatcgtttaa cggtccaggc gatggaataa ttcaacagta tccgggcatg ggaggaaata
      781 tacagggaag gaacggggtc atcattgacg gatttcagcc aggtaaaacc agccatagca
      841 agtaactggc tgtttggccc ggtcttctca ttgtacatga tatccagttc aaggccactg
      901 gtcctgataa attccacatt tttcgccagc gcataacttc ctccgggtac cagattatct
      961 ttccggggca tatccgcata agccgtattg gcccagtcaa tcaggtcctc gtgatcccgg
     1021 taaaaaaaac cggaactgat cctgaaccgg gaagaaaaat tataatccgc cccaaattcc
     1081 atattccatg cccgttccgc tttcagagcc ggattgccaa ttcgtccact gctcaccagg
     1141 gacttgttat aattattgta gcgctctgtg aaatccgcat cgcggaaaga tttacccgcg
     1201 gcggcacgta atgtgagctt cgacaggctc caggccatat ttaactgggg aaccagcacc
     1261 cacccataac tctcatccca atcgaggcgg agactttcat tgatatataa attcctgccc
     1321 gggtggtgac ggagaattcc atacaacgcg ccatgccata aagaatgttc gccgcggtca
     1381 ttggacttaa taaatttcag ggaatgttgc actcctgccg taaacccgtt tttttcatta
     1441 atgattccgg aaaaataagc ctggctggca tagaacccgg cccggttatc gttgggtacc
     1501 gccgtaggac ggaaccagta ctgatcgcgc agttttttat acaccgcatc aatttgtaat
     1561 tgtcccttct tgtacttttt ttgtaacgcc gcctggatcc aggcactgtt tactatttca
     1621 ttggcggtat cactggcaaa actggtatag aaattctgtg cattgaattt ccggaaatcg
     1681 gcagcggtac gtaaactgac ccgccagtta ttggtcagtt gccgtgaaaa ggaaagattg
     1741 gcggtatgca gattgaaata tcctttggtt ccacgaaggg gttgtccatt cgtgttattg
     1801 ctgaataatc cgccgctgaa actgtttttg tgagctgtcc atcgtccata tacagccgtg
     1861 ttcagtagtc cgtattcgcc ggccacaatc cgtccccggt attcattgtt gtattttttt
     1921 cccgaaaatg atttactgat aatatttacc acccccccaa cggcttcgga gccccagacg
     1981 gcagcggctg ccccttttaa tatttccacc cgctcaatct cggcagggtg taaggggata
     2041 tagaggttaa aatgtccggt cagcggatca tttagtttca ccccatctat aattactaat
     2101 acctgctgga aagtaccacc cctgatggtt atgtctgcct gtgccccctg tggaccgcgt
     2161 tgctgtacct cgatccctgg caaataccgg agcaattcat ccactgattg taccggtaat
     2221 gtattaaaag cagccccgtt tatggcaata atattccggc cgctctcttt ttgctgctgg
     2281 gccgtctgtg tggcggttat aatcacatta ctgagcagga gggaggaatc ctgtgcgtgg
     2341 agagaaaatg aaataaacag gaacaggcat aacgcaccgg gtcgcatact tttcatgtgt
     2401 tggttttgcg caaaattagg attaccggtt atttacccgt ttaaaattga acgaatgaca
     2461 gaagctttac aatcggtcag tattgttcct gttcgttggg aaaattaccg gattttacat
     2521 caaggatata ttgctgtacc gcaccggttg cctgttcggc caggttcagg tactgacgta
     2581 agaagcgggg tttgaactcc gtatttatgc ccagcatatc atgcatcacg agtacctgcc
     2641 cgtcacaacc attaccggca ccgattccaa tagtcggaat gctgaggctt gaagaaacgg
     2701 aagtagccag ttctgccggt attttttcta ataccagggc aaaacaaccc gcttgttcca
     2761 acagccgggc atcctccctt agtttgccgg cctcattttc ttcctttgcc cgtacttcat
     2821 aggtgccgaa tttatagatg gattgtggca tcagtccgag atgacccatc accggaatgc
     2881 cggccgagac tattttcttt actgaatcaa ttacttcttc gcccccttcc agttttaccg
     2941 cgtgcgcacc ggtctctttc attatccgga ttgccgaagc taaagcgatt tccgtattcg
     3001 actggtagga cccaaagggc agatcaacca ccacaaaaca acgttcaata ccccggatca
     3061 cggaagaggc atgataaatc atctgatcca atgtgaccgg taaggtggtt tcatgtccgg
     3121 ccattacatt ggatgctgaa tcaccaacca acagcacatc aattccggcc ccgtcaatta
     3181 tccgggcaaa tgaataatcg taggcggtca gcatggaaat tttttcccca gctgctttca
     3241 tcttctgcaa cgtgtgagtg gtgacacgct ttacttcttt tttcacagat gacataactg
     3301 aaaattaaat ggtaaaaagg ttatacttcc agttcacgga gcctgatttc ctcgtaaaga
     3361 ttttggatga gtccgtcggc aagaccgatt ttcggaacat atatctcttc tgcgtcggcc
     3421 cagcgcataa tattaatata aatcagtaat gccggtacaa tcacatcggc ccggtcttcc
     3481 cgaagcttat gcaggctgat ccgctggaaa agggtgagat tgctgaattc tttatagtaa
     3541 tcacgaagca ggtccaggtg aaggggcttt ccgtctttcc tttttgacag ggaaaaaact
     3601 ttattgatat tacctccgga cccgatcgcg gtgacatgat gatagccttt tgttttacta
     3661 cggatatagt ccttcagttc atcccagctg gattcggtca cctggttttt aagcatccgg
     3721 atggtgccga tattaaaaga tttcttgaaa atcaatttgc catcactgaa aaaagtcagt
     3781 tcggtactgc ctcctcctac atcaatatag agataggact catcgttggc catattttca
     3841 gccacatgat tttcgtagat cagggaagct tcctggtcac cgctgataat ttcgatatca
     3901 aggccggttt ctgccttcac ctgtttgatg atatccggtc cgtttgtggc atcccgcatg
     3961 gcggaagtag cgcaggcttt gaggtgtttt acttcatata catccaggag cagacggtat
     4021 gatttgatgg ttttaatgat tttctcggct ttgcctgcag aaatttcccc tttatcgaag
     4081 acatcaaatc cgagccggag tggaacccgc accagcattg tttttataaa ttcagtggtt
     4141 ccctgtggac catttgccac ttccatgatc aatagtcttg ctgcgttgct cccgatatca
     4201 atggctgcca gtttcaattt gggtcagtgt ttttttgtgc aggaatttgt acgtttccac
     4261 ctgcgaccgt attttctttt tttcggttct cacatagtca ttgctgagtt cattgtccag
     4321 tcttcttgcc ttcacattat cttccagctg gatattcagg atgtcaatta gttccttttt
     4381 cagttcggga tgccagaccg gacaggtggc ttccacacga tgttccaggt ttcttaacat
     4441 ccagtcggcc gaagataggt aaactttctg tttgcccttg ttgtgaaata cccacacccg
     4501 ggcatgttcg aggtattcat ccacgatact gatagcccgg attggggact ggtatttttt
     4561 attctccgaa tacaaaccaa aaatgccccg cacgatcagc cggatttcca cacccgcttt
     4621 tgccgcttct tccagtttgg caatcagtat ttcgtctgaa agcgaattca ttttaagaat
     4681 aatagaagcg ggtttctttt tgcgggcctg tttgatttct tcctctataa gccggatcat
     4741 ttcctttttt acaaaatgcg gactgggcaa cacataacgg cagtctttta aaaagtgaag
     4801 gccggtctta ggttgttcga ggaaatgaaa gatccggttt acatccccca tgatctttgg
     4861 attggaggtc agcaggcaat gatccgcgta aatacgggct gtcttttcat tcaggttgcc
     4921 ggtacttaca aagccgtact gcaaaatggt attatttttc gtccgtttac ggatcaggca
     4981 gagtttggaa tgcactttca tatgcgggat ctccaccagc accctggcac cttcttcttc
     5041 caggcggttc ttccattcta tattggcttc ttcttcaaaa cgggctctta attcaatcat
     5101 gacgatcact tccttgccat tccggacggc attgatcagg gcattgataa tcttggactg
     5161 ggaggccagc cggtaacagg tgatttttat ggtcgtgact tccggatcaa aagccgcttc
     5221 ccggagcaga tctatcaacg gtgtaaaaga atgataagga aagtgaagga ggatatcctt
     5281 ttcctggatc aggtccgata cccggcgttc ggtcatcagt ggatgatcaa aaggtttgtt
     5341 ccgctttctt ttttctttaa aaacctcatc cgggaaatcc ataaaatgcc ggaaattatg
     5401 gatccttccg cctggtatca ggttatcctt tttggacaga ttaagtcgtc gtaccaggta
     5461 ctccagcaat cccggatcca tttcgcggtc gtatacaaac cgcaccggtt ttcctttccg
     5521 ccggttcttg accccctttt caatcttctg gataatattg gtcgacaggt catcatcgat
     5581 atcaatctcc gcatcccggg ttactttaaa aatatgggat tggtaatgat catatccgaa
     5641 ataggaaaat atatccggca ggttaaaccg gatcacatcc tccagtaaaa tgatatgctt
     5701 ttcctcattg ccggaaggca gaattttaaa ccggccgtgg atccggctgg ggatctcgat
     5761 gagggcatat tttttatgaa aagcactttc ttttttccac atcactacag ccaggaatat
     5821 ggatttgtcc cgcaacatag ggaacaccgg gatattctca atcatcaggg ggatcaggtc
     5881 ggcactgact tcctcctcat aataagtacg gacaatttcc tgctgttccg ggttcagttc
     5941 cttttcattt cgcaggaaaa tcttttgttt attcagttca accaggcatt cttcccagat
     6001 ccggttgaat tctccctgct ggttcagtac ggtcatctgg atctcgtcaa tgatctgttg
     6061 cggattattc tccaggtgca tccgggcctt ggtaccaaac tgaatcattc gtttcagggt
     6121 ggcgaccctg acccggaaaa actcatctag gttattggaa aaaatgccga ggaaacgaat
     6181 ccggtcacgg agcggaacac tgttgtccgc agcttcctgt aatacacggg cattaaagga
     6241 caaccagctg atatctcggg caatggtttt gtgcttggga ttttccggca taaagggttt
     6301 tgataaactt ttaattgact ataaaattac caaagccgga ttcatttgta tgttaagttt
     6361 taagaaaaaa agggatcatt cccctttcat tttttcaata agggccgttg tagaatatcc
     6421 ctcaagtatc gggttgatca ctactttacc accggccgct atcacttcct ttcccccggc
     6481 cacctgatcc ggggtatagt cccctccttt aatgatcaca tccggcagga tggccgtaat
     6541 gagttcaagc ggggtgtctt cttcaaaaat gaccacggca tccaccatca gcaacgcagc
     6601 caggagaata gcccgggcat cctggttatt taccggacgg ctctctccct tcaaacgttt
     6661 tacggatgca tctgcattta gtccgactac caggtaatcg ccttctttag ccgcttccga
     6721 aagcgaaaaa atatgtccgg ggtgcaggat atcgaaaacg ccattggtga aagccacttt
     6781 tcttcccatg gttttccatt gggtgacctg ctgaacagcg agttcgcgac tgataatttt
     6841 atgtggaatt aaatcggccc gattcatggt tttgcttttt tattaaagta gggcagggcg
     6901 ccgaaactga gaatgccgcc cagtaaaatg acggtggttt gtgccagcca caagatccag
     6961 ccaaaggcca gggcaatggt ataattcaat ccatacaact gcatggtttt ctcaatcagg
     7021 taggcataag cgccgattcc gccgggtgac gcgatcatcc cgatactgcc ggcagacaag
     7081 atggtaaaag cctcttttac cccataaagt tccgtttcct gaaaagccat aaaaccaatg
     7141 taccctccgg ccagataaaa tccccagatc aggaaggtat atactaagaa cagtcctctt
     7201 ttctttagat gacggatcgt actgattccc tgcatcacac tcaggtatac tttccggaaa
     7261 aggaccagta agtcatggat cgtttttttc cggatcagca tccagagacc aatcagaagg
     7321 gctatggcgg ccacaatgat cagggtaacc agcagggtag acactttctt ttctgtttgt
     7381 acctcattgg aactgaaaaa agcttcaatt atttgggaat atagctgcgg ttgaagcagg
     7441 agggtagtac caaataccag cagcagggtc agtgcatcaa tcatccgttc caggataatc
     7501 gttccaatca gtttatccgc aggaattttt tcataccggg ccagcacggt acattttacg
     7561 atttcaccca gcctgggtac ccccatattt accaggtaac cgatcataac agcgaaaaaa
     7621 gaattggccc ttgacggtgt ataacccagt ggctctatta acagacgcca acggatggcc
     7681 cgtacccaat ggctgccgat caaaatcaga aaaaccggaa tagccagcca gtgacgcgca
     7741 ttccggaagg ccattttgat ctgttcggtt ttttcctggt ccaggttttt cagggaaagc
     7801 caggcaaaga aaatacccag gccgagaaaa agaagatact gcaatatggt ccggatattt
     7861 ttcttcatcg ctgcccgaaa gtaaaccgat gaaagcgaaa aacaaaacta tgtggaggaa
     7921 gtccgtgtta attcaagggc aggttatcaa tcagtcggac ttcacccaaa aaggcagcca
     7981 ccagtgccac cagttgttgc ttcccgtccc aataagtaac cggctgaagg gtagtggcat
     8041 cggcgatcac gatatagtca gtcctgaatc cggctgcctg aagctggtca aaagcggatt
     8101 tttgaatcac atccagctta cctgctttca ggttttcttt caggaaggat aatgtccggt
     8161 agatcgttac agatattacc cggtcagctt cgtttagtct cctgttccgg ctgctcattg
     8221 ccagtccatc aggctcacga agggtggggc agatatggag ttgaattttt tcgcagttgc
     8281 ctgtttgttg taacagccgg ttaatcacca tgcattgctg gtaatctttc tgacccataa
     8341 aaagtgcatc cggttcaacc tgtaaaagca gttggtttac gacctggcag accccctgaa
     8401 aatgaccggg tctgaatttc ccttccagca gggtctccat ctcgccaagg tcaaattttt
     8461 gattggaggt tagtccatcc gggtacattt cgtccacatc tggcaaaaac agaaggtcac
     8521 atccggcgga taaaaggagg gcaatgtctt tatcagttga caggggatac ttttcaaagt
     8581 cagcaggatc gttgaactgg gtggggttta caaatatgct gcaaacggtc agggtggttg
     8641 ccgattttga ggcttggatc agggataaat gcccttcatg caaagccccc atggtgggaa
     8701 caaatcctat ttttttgcct gattttcgtt gaacatcaag atgtttacgc aggtcaattg
     8761 cttttttaaa taaaatcatg cgatgatagt ctttgacggt taataggctt taaaaaatat
     8821 aattcggccg aaatttgtta cctttgcacg cctttaggcg attgtaccgg gaggaaagcg
     8881 ggtgtttttc ccgccttagc cgggtacaaa aaaaggacgc acatgtcagc aaagaaaaga
     8941 attttattta tcgcgaacga aat
//
LOCUS       DAOPMW010000105         8949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8588_length_8949_cov_7.559928, whole genome shotgun sequence.
ACCESSION   DAOPMW010000105 DAOPMW010000000
VERSION     DAOPMW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8588_length_8949_cov_7.559928"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLU11_06825"
     CDS             complement(<1..552)
                     /locus_tag="PLU11_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPN58867.1"
                     /translation="MAGFTILGKAEKGNRYDAIVIGSGITGGIAAKELTEKGLKVLVL
                     ERGRMVEHIVDYPTAQKDPWEFPLANMVSLEEKKEYAVQKDIYIFGQDAKHFLVKDTE
                     HPYVQEKPFSWQRGYQLGGRSLLWSKHCYRLSDLDFEANQKEGIGIDWPIRYKDIAPW
                     YDHIESYVGISGNRDGIAQIPDGN"
     gene            complement(562..1107)
                     /locus_tag="PLU11_06830"
     CDS             complement(562..1107)
                     /locus_tag="PLU11_06830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HPN58868.1"
                     /translation="MERRDLLKSIGLIGGSSLFPAVLSNFLSSCSGKDMSAYTPVFFS
                     KNEYNQITEMIDLLIPATGTKSASQVNVQVFLDQVFQQCLVKEEQQEIHDGLKSIETG
                     FDTAKDKQAFVKELDEKAFGKKEANAWFRSIKKYTLIGFFTSEEGVTKASNYVKAPEA
                     YKGDIPADETTLNYGRTTLHF"
     gene            complement(1217..3274)
                     /locus_tag="PLU11_06835"
     CDS             complement(1217..3274)
                     /locus_tag="PLU11_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58869.1"
                     /translation="MFRLRKKSPDFFETALLQDRIKADERSTGLSFLVSFFEQIRPGH
                     TLKKKNAEANLRFAMQELKLHPELLANLQQALLSQLISTKLTSAITESGIPLSRGFWQ
                     ELVNRLKHKLIPELQDETDFLYVINSVFYKKADYQWVESIPRSLWIDFFETLGFRFST
                     DAHRIKNELLSSLKILSFQVAQLGLEREVLNYLPENERNDHTAFVRQNYLVHELELIL
                     NENKEANAISSTSTALKEHIHSCYTLLDYIRESHADKGASIQQTYLLLILTNRLERML
                     LIADILDADNQFDTGRFTDLFRLLIRNENRKNSIMEFLSQGLGYLAYQIAEHKGSKGH
                     KYITETRAEYYKMIRSAMWGGLIISFTAILKNLIGKLKLAPFPLGFLYSMNYSAGFIL
                     IEETKSTLATKQPAFTASAVAGSLDTRKLGGEPDLDGLAATVAKVSRSQIASFFGNLI
                     IVFPMTFLLAWGYHQVTGSKIATDAAAFKLLKDQHPWRSPALLYACFTGLFLFISGII
                     AGYWQNKVQYGQIRERLIRHPALKVTMSPQRRTRLANYVEKNIGALIGSIALGFFLGF
                     SGVIGKIFGIPFDIRHITIASGNVSIGLYGVGINEVPAYFMLAVFFGVLGIGVLNFLV
                     SFALAFTVAVKSRGIHLSQYPRLLSTIGKYLFRHPRVFFLPERKSSVQNYHPLAGKDD
                     SAL"
     gene            complement(3321..3710)
                     /locus_tag="PLU11_06840"
     CDS             complement(3321..3710)
                     /locus_tag="PLU11_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58870.1"
                     /translation="MKIFYFTFLLLAVSNLASAQGPLPDCPSFHTGEYLYTDSTGDTW
                     ELRRKKKIQTERNTRNGKTIKFKILWLSDCEYRLTQTWTNIRTRRKWNRAHFSYRIIR
                     TTPNSYTYDCSCKDSPAIAGTVVRVMN"
     gene            complement(3707..4948)
                     /gene="hutI"
                     /locus_tag="PLU11_06845"
     CDS             complement(3707..4948)
                     /gene="hutI"
                     /locus_tag="PLU11_06845"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPN58871.1"
                     /translation="MSATLIRDIKLLVNTPETDTCLRGKELAVLPVIENAYLIMEDEF
                     ITGLGRMEELIYPEKHFKSVINATGQIVLPAWCDSHTHIVFAATREEEFIDKLKGMSY
                     ADIAAKGGGILNSARKLGEASESMLFNDAWKRLEEVSKMGTGSIEIKSGYGLTVESEL
                     KMLRVIKKLKEKSPLTIKATFLGAHTYPPAFKEDHEGYIRQIIDEMLPVIAKEKLADY
                     IDVFCETGFFSREETIRIGKAGMAIGLKPKIHANQLNLSGGTEAGVELGALSVDHLET
                     MEESTIRLLAGSSTIGTLLPTAAFFLRMPFQPARQLIDAGCALALASDYNPGSSPSGN
                     MNLVTSMSCIQMKMLPEEVINAATINGAFAMEVQDKTGSIRPGKKANLIFTQPVPNLA
                     TLFYSFGTNHIEKVILNGKIV"
     gene            complement(5034..5969)
                     /locus_tag="PLU11_06850"
     CDS             complement(5034..5969)
                     /locus_tag="PLU11_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58872.1"
                     /translation="MKTRILSIAIAAFSFTFLFTSCKKEASNAGSTDFTAETSTHSDD
                     QSRFSGEVDAVANDANLLLESTAGITGRPGDLNSLICDADIVVDTAANPRTITITYNG
                     TSCLGNRTRTGVIVISMAQGVRWSQAGAAISLTYQNFKVTRTLDNKSITVNGTHTLTN
                     VSGGLLINLPVLNTITHSITSSNMSVTFDNGTQRTWQVARQRVFTFNNGVTITITGTH
                     ADGGVTGIAEWGLNRFGRPFTTTIAEPVVLKSDCSFRAGSGKVVHNTTSYNAAVTFGL
                     DATGAATSCPGAGNYYMKIVWTGPAGNSHTALLPY"
     gene            complement(6198..7379)
                     /locus_tag="PLU11_06855"
     CDS             complement(6198..7379)
                     /locus_tag="PLU11_06855"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPN58873.1"
                     /translation="MKEVVIISAVRTPIGSFGGSLKSLSATQLGAIAIKGALEKSSLK
                     PSQVEDVIMGCVVQSNLGQAPARQASKFAGLPNEVNCTTVNKICASGMKAIALAAQSI
                     ALGDADIVIAGGMESMSNAPFYNENMRWGHKYGNVTLVDGLAKDGLTDVYDGKPMGHA
                     AELCASECGVTRADQDAFAIESYKRSQAAWEKGLFDKEIVPVEIPQRKGDPIVFSKDE
                     EPYNVKFEKIPTLNPAFIKDGTVTAANASTMNDGAAAILLMSAEKAHELGLKPIGRIR
                     GYADAEQAPEWFTTSPSVAVPKAVKKAGLSMTDIDYWELNEAFAVVGIENSRRMKLDP
                     AKVNVHGGAVSLGHPLGCSGARIIVTLLHILKSNNARFGAAGICNGGGGASAMVIENI
                     A"
     gene            complement(7441..8253)
                     /locus_tag="PLU11_06860"
     CDS             complement(7441..8253)
                     /locus_tag="PLU11_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN58874.1"
                     /translation="MEKITGLAGNQFNKTLPGWNKINPMCQLILQMSRYTVILLLIFC
                     HPALVCSQTSILGKIYDGKNDSLLAGVTVMNITTKKFVLSDRNGQYTVSAVENDLLVF
                     SFSGYAPDTISVEPYMFDTRFDITLQPQPLLLKGVTVVATNYQTDSLNRQAYYRHILS
                     KREPGLTGRNTPTGFGISLSPISHFSRESRERRALKKRIAREEKESYIDHQFSPELVK
                     RITRLEGDSLRLFMYTKRPSYDFCRKTSPEEMLVYISDCLKEFRKPAEKKPK"
     gene            complement(8225..>8949)
                     /locus_tag="PLU11_06865"
     CDS             complement(8225..>8949)
                     /locus_tag="PLU11_06865"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPN58875.1"
                     /translation="VNPYSALMQFRGFTTDSSKRNFMPAHDNGTAAADPGYMDRLWRG
                     TTSVEAADKEGWVVSITPSGGWIPACIAGNTGVGMSQRMQSFVLDSTLNPFNVVAPGK
                     RPRVTLTPSMALKDGKPYLSFAVQGGDTQDQNLLQFFLNMVEFGMTVQQATEAANINS
                     NQLWLSLGGTKTDDRKPRPGSILLHDTIPEKVRNELKKMGYTLSFDDRTSGPINAIYF
                     DWKHGSFWGGSSNHGEDYGIGW"
BASE COUNT         2318 a         2135 c         2014 g         2482 t
ORIGIN      
        1 attcccatct ggtatctgtg cgattccatc ccggttgcca ctgatcccca cataggattc
       61 aatatgatca taccagggag caatatcctt atagcggatc ggccagtcaa tcccgatccc
      121 ttctttctgg ttggcttcaa aatccagatc actcaggcga taacaatgct tggaccacaa
      181 taaggaacgt ccgcccagct ggtatcccct ttgccaggaa aatggttttt cctgcacata
      241 cggatgctcg gtgtctttta ccagaaagtg ttttgcatcc tgaccgaata tgtaaatatc
      301 cttttgtacc gcgtactctt tcttttcttc caggctgacc atattggcta acggaaattc
      361 ccagggatcc ttttgagcgg tcgggtaatc cacgatatgc tctaccatcc tgccccgttc
      421 cagcaccaat accttcagtc ctttttccgt gagttctttg gcggcgattc caccagtgat
      481 accagaacca attacgatgg catcgtagcg gtttcctttc tctgcttttc ccagtatagt
      541 aaatccagcc atggtattca attaaaaatg taaagtggtt cttccgtaat tcaaggtcgt
      601 ttcatcagcc ggaatatccc ctttataagc ttccggtgct ttcacataat tggaggcttt
      661 tgtaacaccc tcctcggaag taaaaaagcc aatcagggta tattttttga tgctgcggaa
      721 ccaggcattc gcttcttttt ttccaaaggc tttttcatcc agttctttga caaacgcctg
      781 cttgtctttt gccgtgtcaa aaccggtttc aatgcttttc agcccatcat ggatttcctg
      841 ctgttcctcc ttcacgaggc attgctgaaa gacctggtca agaaaaacct ggacattcac
      901 ctgcgaggcg gatttggtac cagtagccgg aataaggaga tcaatcattt cagtgatctg
      961 gttgtactcg tttttactga aaaaaaccgg ggtataggca ctcatgtctt tgccggagca
     1021 actgctcagg aaattggata aaacggccgg aaacaaactg ctccctccga tcaggccaat
     1081 tgattttaaa agatcccttc tttccatttt gttaattgaa gtgggttgac aatccaatat
     1141 gttcaaatgt aattattctg gtaatccggt gtactgatac aaatcagggc tgctatttgt
     1201 attattctcc tggaaattat agtgcactat catctttacc agctaaagga tggtagttct
     1261 gtacggatga tttccgttcc ggcaggaaaa aaacacgcgg atgacggaac aggtatttac
     1321 caatagtact gagtaaacgg ggatactgcg ataaatgaat gcccctggat tttacagcta
     1381 cggtaaatgc cagggcaaaa ctgaccagaa agtttaatac gccgataccc agtactccaa
     1441 aaaatacggc cagcataaaa taagccggca cctcattaat tcccacacca tacaatccaa
     1501 tggatacatt tccggaagca atagtgatat gacggatgtc gaacggaatg ccaaatattt
     1561 tccctatcac tccggagaag ccaaggaaaa acccaagagc aatactacca atcagggcgc
     1621 ctatattttt ctctacataa ttggcaaggc gggtccgccg ttggggtgac atggtgactt
     1681 tcaatgccgg atgccggatc aatcgttccc tgatttgtcc atattgcact ttgttttgcc
     1741 agtaaccggc tatgatgcca ctgataaata aaaataatcc ggtaaagcag gcatacagaa
     1801 gagccgggct tcgccaggga tgctggtctt tgagcaactt aaaggctgcg gcatcagtgg
     1861 caatcttgct gccggtgacc tgatgatagc cccaggccag taaaaaagtc atcgggaata
     1921 caataatcag gttaccgaag aaagaagcaa tctggctgcg tgaaactttc gcaacggtgg
     1981 ccgccaatcc atccagatcc ggttctcctc ccagtttccg ggtatccagc gaacctgcca
     2041 cagcactggc tgtaaaagcg ggttgcttgg tggccagggt tgatttggtt tcttcaatca
     2101 ggataaagcc ggcgctgtaa ttcatactat aaagaaaacc caaagggaaa ggggccagtt
     2161 ttaatttccc gatcaggttt ttaaggatgg cagtaaatga aatgataagg cctccccaca
     2221 tagcggaacg aatcatttta taatattccg ctcttgtttc tgtaatatac ttatgcccct
     2281 tgctgccttt gtgttctgca atctggtaag cgagatagcc cagtccctgg gagagaaact
     2341 ccataatact gtttttccgg ttttcattcc ggatcagtaa ccggaacaga tcagtgaaac
     2401 ggccggtatc aaactggttg tcggcatcca gtatatctgc aatcagcaac atccgttcca
     2461 ggcgattggt cagaatcagc aacaggtagg tttgctgaat actggcacct ttatccgcat
     2521 ggctttcccg gatataatcc aataacgtat aacaggaatg aatatgctct ttcaacgcgg
     2581 tactggtgct gctgatggca ttcgcctctt tattttcatt gagtattaat tccagttcat
     2641 gcaccaggta gttttgccgt acaaaagctg tatgatcgtt tctttcattt tccggcaggt
     2701 aattcagtac ttcacgttca agccccagct gggcgacctg gaaggataat attttcagtg
     2761 aactcagcag ttcattttta atccggtggg catcggtgct gaaacgaaaa cccagggttt
     2821 caaaaaaatc aatccataat gaccggggaa tactctctac ccactggtaa tccgcctttt
     2881 tatagaaaac actgttgatt acatacagaa aatccgtttc atcctgtaat tcgggaatca
     2941 gtttatgttt caaccggttg accaattcct gccagaaacc cctggataag ggaataccac
     3001 tttccgtaat ggctgaagta agtttggtgc tgatcagctg ggagagcagt gcctgctgaa
     3061 gattggccag taattccgga tggagtttca gctcctgcat ggcaaaccgg agatttgctt
     3121 cggcgttttt tttcttaagt gtatggcccg gcctgatctg ctcaaaaaaa gatacaagaa
     3181 atgaaagccc ggtggaccgc tcatctgcct ttatccggtc ctgcagcagg gctgtttcaa
     3241 aaaaatcagg actcttttta cgtaatcgaa acatgttccc gaatatacaa catgtgggtt
     3301 gtaagcatgt aaactgtgga ttaattcata actctcacta cggtacctgc gattgccgga
     3361 ctgtctttac aggaacaatc gtaagtatag ctgtttgggg ttgtacggat aatccggtag
     3421 ctgaaatggg cccggttcca tttgcgccgg gttcgtatgt tggtccaggt ctgtgtaagc
     3481 cggtattcgc aatcactcaa ccacagaatt ttaaatttga tcgttttccc gtttcgggta
     3541 ttgcgctcag tctggatctt ctttttccgc cggagttccc aggtatctcc cgtggaatct
     3601 gtatatagat attcaccggt atgaaatgaa ggacaatcag gtaaagggcc ctgtgcggat
     3661 gccaaattgg aaacagctaa gagaaggaag gtgaaataaa aaattttcat actatttttc
     3721 cattcaggat tactttctca atatggttgg tgccaaaaga ataaaagagc gtggccaggt
     3781 tcggtaccgg ttgagtgaaa atcaggttgg ccttttttcc aggacgtata ctacctgttt
     3841 tatcctgtac ttccattgca aaagcgccgt taatggtagc ggcattgatc acttcttccg
     3901 gcagcatttt catctgtatg cagctcatcg aagtaaccag attcatattt ccagaaggag
     3961 aagaacccgg gttgtaatcg gaggcaaggg caagggcaca tccggcatca atcagttgcc
     4021 gggctggttg aaatggcatc cggagaaaaa aggcggccgt tggtagtaaa gtaccaatgg
     4081 tggatgagcc ggccagcaac cggatcgttg actcttccat ggtttccagg tgatccacac
     4141 tcagggcgcc caattccaca cctgcctcag tacccccaga caggttcagc tgattggcat
     4201 ggatctttgg tttcagacct atggccatac cggctttgcc aatacggatg gtttcctccc
     4261 tggagaagaa accggtttca caaaacacgt caatataatc ggccagtttt tcttttgcaa
     4321 ttaccggcag catctcatca ataatctgcc ggatatatcc ttcatggtct tctttgaaag
     4381 ccggggggta agtatgggct cccaggaaag tggcttttat tgtcaacggt gatttctctt
     4441 tcagtttttt aataacgcgg agcatcttga gttcactctc cacggttagt ccgtatccgc
     4501 ttttgatctc aatggacccg gttcccattt tactgacttc ttccagtctt ttccaggcat
     4561 cattaaatag catcgattca ctggcctccc cgagtttacg ggccgagttg agtatacccc
     4621 caccctttgc tgcgatatcc gcatagctca ttcctttgag cttatcaatg aattcttctt
     4681 cccgggtagc cgcaaaaacg atatgggtat gactgtcgca ccaggccggt aaaacgatct
     4741 gaccggtggc attaatcact gatttgaagt gtttctccgg ataaatcagt tcttccatcc
     4801 gccccagtcc cgtgatgaac tcatcttcca tgatgagata cgcgttttca atcactggca
     4861 atacggccag ttcttttcca cgcaggcagg tatccgtttc cggtgtattc accaataatt
     4921 taatatcgcg tataagtgtg gcggacatgg ccctaaatta gcgaaaacga aacgaagtca
     4981 aaaaaaatgc cctgccgtta agggcagggc accggataag gtgtaagaaa atattagtag
     5041 ggtaataatg ccgtatggct gttaccggcc ggaccggtcc atactatttt catatagtag
     5101 ttgccggcac ccggacagga tgtggctgct ccggttgcat ccagtccgaa tgtaacagcc
     5161 gcgttatagg aagtagtgtt atgtaccact ttgccgctgc ctgcccggaa ggaacaatcg
     5221 cttttcaaaa caaccggttc agcaatggtg gtggtaaacg gacggccaaa acggttcaat
     5281 ccccattctg caattcctgt aactccgcca tccgcatgtg taccggtgat ggtgattgtt
     5341 actccattgt tgaaagtgaa tacccgttga cgggcaacct gccaggtacg ttgagtgcca
     5401 ttgtcgaaag taaccgacat attactgctg gtgatggaat gcgtaatggt gttcagaaca
     5461 ggcaggttga tcagtaatcc accgcttaca ttggttaatg tatgtgtacc gttaacggta
     5521 atacttttat tgtccagggt ccgggtgact ttaaaattct ggtaggtcag gctgatggct
     5581 gctcctgcct ggctccagcg tacaccctgc gccatggaaa tgacaatcac acccgtgcgg
     5641 gttctgtttc ccaggcaact ggtgccatta tacgtaatgg taatggtgcg gggatttgcc
     5701 gcagtatcca ccacgatatc cgcatcgcag atcagtgaat tcagatcacc gggccggccg
     5761 gttatgccgg ctgtggattc caggagcagg ttcgcgtcat tcgctaccgc atccacttca
     5821 cctgaaaacc gggactggtc atcggaatgg gtggaagttt cagccgtgaa atcagtgctg
     5881 cctgcattgg atgcttcttt cttacaggaa gtaaaaagaa acgtaaaaga aaatgccgca
     5941 atggcaatgg ataaaatcct tgttttcata ttcgggtttt aaatgttaat ctcaataaat
     6001 aacagacaat agacagggac acttgtcaat tggtttaaca agaaccctgt tttttttcag
     6061 gaaaaagaat actaaccatt aaaacgggaa taagccgggc ttagtacggg cccgcccggg
     6121 aaaaaatacg atgaagacga tgaaagatta cgattgggct gtaataatgt gtaagccgtt
     6181 taataaccgg ggatggttca ggcaatattt tcaataacca tcgcactggc accaccgcct
     6241 ccattacaaa tacctgcggc gccaaaccgg gcattattgg atttcagaat atgaagcagg
     6301 gtgacaataa ttctggcccc gctgcatccg agtggatgac ccagggatac cgctccccca
     6361 tgtacattta cttttgcagg atccagcttc atgcgacggg aattttcgat ccccaccacc
     6421 gcaaaagctt cgtttagttc ccagtaatct atatctgtca tagacagtcc ggccttttta
     6481 acggctttgg gcaccgctac ggaaggagaa gttgtaaacc attccggggc ttgttcggca
     6541 tcagcatatc ccctaatccg gccaatcggt tttaatccca gttcatgtgc cttttctgca
     6601 ctcatgagta aaatggccgc cgccccatca ttcatcgtgg aagcattggc tgccgtaaca
     6661 gtgccgtctt ttataaaggc cgggtttaaa gtaggaattt tttcaaattt cacattatag
     6721 ggttcctcat ctttcgagaa aacaatgggg tcgcctttcc gttggggaat ttcgacagga
     6781 acaatctcct tatcaaacaa ccctttttcc caggcagcct gtgaacgttt atagctttca
     6841 atagcaaatg catcctgatc tgcacgggta acaccacatt cactggcaca aagctcagcc
     6901 gcatgcccca tcggtttacc atcatataca tcggtcaggc catcctttgc cagtccatca
     6961 accagggtta catttccata tttatggccc caacgcatat tttcattgta aaaaggagca
     7021 ttgctcatac tttccatacc gcccgctatc acaatatccg catcacccag ggcaatactc
     7081 tgagcggcca gtgcgatggc tttcatgcca ctggcacata ttttattgac cgttgtacaa
     7141 ttcacttcat tcggtaaacc ggcaaatttt gacgcctgcc tggcaggtgc ctgacccaga
     7201 ttggattgaa ccacacagcc cattattaca tcttccacct gggagggttt gagactggat
     7261 ttttccagtg cacctttaat ggcaatggca cccaactgtg tggcagacaa tgacttcagg
     7321 cttcctccga aacttccgat gggagtcctt actgcagaaa taataactac ttctttcata
     7381 gtgaatagtg taaaattatc ctgcaaagat aacgattgtt agtgtgagat gaaagctttc
     7441 ttatttcggt tttttttctg caggttttct gaattctttc aggcaatcgc tgatataaac
     7501 cagcatttct tcgggactgg ttttacggca gaaatcatag gagggtcgct tggtatacat
     7561 aaataaacga agcgaatctc cttccagccg ggtgatccgt ttaaccagtt ccggggaaaa
     7621 ctggtggtcg atatagcttt ctttttcttc ccgggcgatc cgttttttca gcgctcttcg
     7681 ttcccttgac tcacgggaaa aatgactgat cggactcagg ctgatcccaa agcctgtggg
     7741 tgtattacga ccggtaagtc cgggctcccg cttacttaaa atatggcggt agtatgcctg
     7801 acggttcagt gaatcagtct ggtaattcgt tgcaactacc gtcactcctt ttaataaaag
     7861 aggctgtggt tgcagggtaa tatcaaaccg ggtatcgaac atataaggtt caaccgatat
     7921 tgtatctggg gcataaccgc tgaaactgaa aaccagcaag tcattttcaa cggctgaaac
     7981 agtgtactga ccattccggt cagaaagtac aaatttttta gtggtaatat tcatcacggt
     8041 cacgcccgca agtaaggaat catttttgcc atcataaatc tttcccagta tggaggtttg
     8101 ggagcataca agagccgggt gacaaaaaat aagcagtaag atcaccgtat atcgagacat
     8161 ctgtaaaatt agttggcaca tggggttgat cttattccat ccgggcaaag ttttgttaaa
     8221 ctgattacca gccaatcccg taatcttctc catgattact gcttcctccc cagaaactgc
     8281 cgtgcttcca gtcaaaataa atggcattga tgggaccact ggtccggtca tcaaaactca
     8341 gggtataccc cattttcttc aattcatttc tgactttttc gggaatagta tcatgtaaca
     8401 aaatacttcc gggcctgggt ttacgatcat ctgttttggt tccgcccagt gaaagccaga
     8461 gctgattact attgatattg gctgcttcag tggcctgctg caccgtcatg ccgaattcta
     8521 ccatattcag gaaaaattgc agcaggttct ggtcctgtgt atcgccaccc tgcacggcaa
     8581 aggataaata gggttttcca tcttttaaag ccatggaggg cgtcagggta accctcggac
     8641 gtttgccggg cgccaccacg ttgaatggat tcagcgtgga gtccagtaca aaactctgca
     8701 tcctttggct catacccacc ccggtgttgc cggcaataca agccggtatc caccccccgc
     8761 tcggtgtgat ggataccacc catccttcct tatcggcggc ttccacactg gtagtgcctc
     8821 tccataagcg atccatatat ccgggatcgg cggctgcggt gccattatca tgcgcaggca
     8881 taaaatttcg tttactgcta tccgtagtaa agccccggaa ttgcatcagg gctgaatacg
     8941 gattaactt
//
LOCUS       DAOPMW010000106         8765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8853_length_8765_cov_7.744546, whole genome shotgun sequence.
ACCESSION   DAOPMW010000106 DAOPMW010000000
VERSION     DAOPMW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_8765_cov_7.744546"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1101..2909
                     /locus_tag="PLU11_06870"
     CDS             1101..2909
                     /locus_tag="PLU11_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58876.1"
                     /translation="MKRKLIPIAFLLLTSYFGFSQTTSGTYNTDFNEMTIAQNGSKVT
                     GTYKHQNGRVEGTLSGHTLTGFWFQSNGKGKLIFEFNADFTAFTGKWSYNDATPSSKW
                     NGTRIAGLETKSQPSQTAQQGSQPKAQKTATAAAATKSADTKDNLNKADVATGSELQK
                     QISSQVKGNGNEAESNSTSANSIRKPLSEYEKSLVKEAKSDLKKAISEEDITDTERTR
                     MVVKSAKTLKEYGQPAAFPDGDIPLKTSREKEYKRIKEEVVSANDLRKLLSEAQLAEK
                     MSLINSMQIDILEEQIKILIPGKAPFDLSKDLVGTVFNISITEGMSGGQQQGAKDLVQ
                     KFRDLALTNEFVAYAERLYLQQQDLMKKADLNIEKAKKLQAQLRKSYQDAENSTFTFR
                     EFSPAGASPAPGPRSSTASKGQGKGLWVLVKTIEYDGKTELDHTNKNGVYRVSGNSSA
                     GAFIYTWKYLGKADNYYNPPLLSDENSTIKCNVSAPPKVMDAGEIISLKISMSFAAQK
                     LSYFTSGATVSADFDKPDLGIGFASGSNISFVNNAGKSSFKINTHKSVKIYTVDEIVT
                     ATAPAGVKDMRIAIRASFNPGAKMGTCYIYEWKYAY"
     gene            3087..5057
                     /locus_tag="PLU11_06875"
     CDS             3087..5057
                     /locus_tag="PLU11_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPN58877.1"
                     /translation="MKKNLLCLFLLCTTVFHGLSQDKMALLVAIGQYPPASRIRPIAS
                     VTDLKYLRTALNKHGFADKNIQTLINAKATKQGILTSLTAMARKAKKNDIIVLSFGCH
                     GQQIRDQRTVELGKDEDDGYDEALVPYDAKGTYSPTGYRGEKHLRDDDLYPILMEIRL
                     KIGKDGSLLVLIDACHSGTGTRADDFPASRGEPVPFPDPENPYNAADISDLDKKETFL
                     DIQPDSLSNMVVISGSGPHQINKQVLVNKEEVGSLSYGFYKAMSELTAGHDYAVLYEK
                     IKSSIQAIIPDQLPMIEGNTSQEIFSGRYLPVRERNIIRVGLKEAASNSSDSVFTLNR
                     GMMDQLSVGVTGTIYPLGKETPVATAVIRKTSHFSSVGVASVLLKKSETYELRLTEAN
                     YGNLSALLQFDKQDKLPSGTEKQVRKLLSPYAYISFSDQRADFQLRQSATATGRQVEL
                     TDRNNQLVWSAPLTPADTVAAADQLILIRGIRNALQVKYLRTMPDGGELSQWVTAEIL
                     ANNGGRTALEFTEGDQYTLKLVNNSEYKLFYTVLDIYPDNKVDVLYPYKGKEPADYSM
                     EKKNPVERKLTVSKGSPTGVEFLKIIVSKEPMDLRGVFEQKIQRDNMMSFQVMLDDLF
                     SRPEGGETVRADVSAIKMEEIGIITVNFMIRK"
     gene            5065..>8765
                     /locus_tag="PLU11_06880"
     CDS             5065..>8765
                     /locus_tag="PLU11_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58878.1"
                     /translation="MQLLKKTGLSLLFIFTVVFTIAQEIPRTEARVVWLPIDRTYQDK
                     DSLIAFLSGVSNIGLQNRQILKGYQSSVDEIPGIRPKKDFMETGAGFIYSTDSIQVAF
                     IRMYNPADTLGRGDMISLQMQVPQLPYRGVYSELAFNGIIFTDAEKNPLYTLDYLFHH
                     DSRKLEDSLFGVIIKSLHSTYELVKDRPELPASLLAKVTAGRFKNRIPLEIIRDVTRK
                     DLEAFFMYTQAYPVGYMGKNYRASESFAGWLISNSPYSVGEVKQGLFPVYKNPVEFKK
                     RLSEYKTDVLNEHTATSLATDAIGLSNQLKFTEAQQLSDFSIALAEGVNDTMSKPTVY
                     LCKAQLFLDQEKYPESVTWCDKAIKAALLAGDRDIEIQAIIKKGYCYYKLSKYQGTET
                     MMSNAVDKLNQHRATLGEKVYNNNYRKILEYRSSIRNNAGRYDEALQLLDSAIRINNR
                     INSYDAQLANAGYYAFIGRVYNDQGKPADAVPALKQSVTIYRNNSDVLNSAIVENDLA
                     YSYYKQGNYRQSLEQAGMALNKLVRLDDFNNAGYSMSLTGSCYWQMGLYDSALSAHHA
                     SLALREKSGNLSGQAYSWTRLGDLYKESGSKKQSLLAYEKAADLYRKLMDSTGLAEVF
                     NARGQVYLDDENFKNAASEFEKANGVSHKSTVEALYKLGIAWSSIDTVKARKYFQEAR
                     RNSIDDGNTGYQFASSRLLARMAYLNQDKRLGERYYNECVALGKEIKTAFADAQCLSL
                     TAYKFECETQLDSALFYYRKAMTITDTVDRGVSVGHLNSIASIFISMGEFRKAEDALN
                     SAIRVARDISDSLSLGSTLQFSSFLYSRTAEFARGLANNDSAIAIFKNSGHLIRLAGT
                     YASRATLYSSMGENKESILANLYADSLYNEEMQKEQQGLLYNNIGIVYISQGDYPTAM
                     KYLLKSLTMLPKVTNNEDYLLVQGNMAECYIGLKKYKEARTLLMEFLPLTRKMKLNRI
                     ASGMALVFGKLSLQENKIADATRYYTEAWLFGEASGEKEKALEALINLGRIYRKEGNK
                     DTAAARIREAVALSVKYKLAGGWEAFYESGLFSYEAKQYDTAVVYFKEAVGLLETLTD
                     KLFGGEAAKKIFNNDPRKSDLFNKITFSYYNLGDINQAWSYANRSNIAGIRELSGSLT
                     TNSSDAEKNEALKKLFSLQESKKALEGTLEKQEGVARQETLKKIEILEADYNNFLSDV
                     VELYPDLGTYFSKSNADEFNKYKSKLPADV"
BASE COUNT         2642 a         1802 c         2094 g         2227 t
ORIGIN      
        1 aaagggctac ggcggataaa tccgctcagc tctcatgccc tccggctatt gcgaaggagg
       61 gcttcgccct aaaggttatc cttcattgtt ttccttcgcc ctccgaagca atagctacgg
      121 agggcatttt tttgaatatt attgctaaga aacaatatca aagggctacg gcggataaat
      181 tcgctcagct ctcatgccct ccggctattg cgaaggagtg ttcgccctcc gaagtttaaa
      241 tttcggaggg ctttttattt aatcttcttt ataagaagat attcgcaagg gctacggcgg
      301 acaagtccgt cttcgacctc cgaagcttca gcgaaggagg aatcaaagcc ctctggctat
      361 tgcgcaggag ggattcgccc tccgaagcta aagctacgga gggcttttta tttaatctgc
      421 ttaataagga gctggcggta atttccagac atgccgctca aacctgaccc caaaatcatt
      481 tattttctca agcattcgct caattcttgc cttgcaatga cgatacatta aaagccattt
      541 aaatcataac cggcataaac tttaaccgca ttccataata cggcaccaaa acttttacaa
      601 ttcaagactt ttacaaactg gacagttgtg aggatagaaa gctttggtat ccggtcaatc
      661 aaaatataaa cacaacaaat cttacaagtt ccgggaacaa taatgcacgt ataaatactc
      721 aagaagctca tcctgacttc atggcgctgg ttccgggctg aaggattact tatatacata
      781 tactatgtat ttgaaacgtt gtagctttac agggcattgg caggcggcag acaccgcaat
      841 gaattccagc cacatcgtca atgctttaac gttatacgtc atgcctcgac gaaaaaataa
      901 tacggtgaag tataaaaccc ctcatacgtt attttctttt ttatttaaat atagatcgta
      961 tacattttgt tgttatactc atcgggaata gtaattcttt gtcttgtaga acgaaagttg
     1021 cctaatcggc ttcttatttg ccaagccttt agaatttccc tagatgcatg aaataacaat
     1081 cactttctaa attcatagaa atgaaaagaa aactcattcc aatagccttt ttattgttaa
     1141 ccagttattt tggattttca caaactactt cagggactta caataccgat tttaacgaaa
     1201 tgactattgc tcaaaatggc agcaaggtta ccggaactta caagcatcag aatggccggg
     1261 tcgaaggcac tttgagtgga catacgttaa ccgggttttg gttccagagc aatggtaaag
     1321 gaaagttgat ctttgagttt aatgctgatt ttacagcttt taccgggaaa tggagttaca
     1381 atgacgctac tccttcaagc aaatggaacg gaacaagaat tgcagggctt gaaacaaaat
     1441 cccagccatc acaaacagca caacagggtt cacagcccaa agcacaaaaa acagcgacgg
     1501 cggccgctgc aacgaaatct gcagatacaa aagataattt aaacaaggcc gatgttgcta
     1561 caggcagtga attgcagaaa caaatctcat cacaggtaaa gggaaacgga aatgaggctg
     1621 aatcaaacag tacgtctgca aattcaatta gaaagccact ctctgaatat gaaaaatccc
     1681 ttgttaaaga agcaaaaagc gatttaaaaa aagccatctc ggaagaggat ataactgata
     1741 cagagcgaac aaggatggta gtgaaaagcg ctaaaacact caaagaatat ggacagccgg
     1801 ccgcttttcc cgatggagat attcccttaa aaacgagtcg cgaaaaagag tacaaaagga
     1861 ttaaggaaga agttgtttca gcaaatgact tacgtaaact gctaagcgaa gcccagctgg
     1921 cagagaagat gtcgctcatt aattcgatgc agatagatat cctggaggaa caaataaaga
     1981 ttctgattcc cgggaaagca ccttttgatt tatcgaagga cctggtaggt actgttttta
     2041 atattagcat tacggaggga atgagtggag gacagcaaca gggtgcgaaa gacctggttc
     2101 aaaaattcag ggatttggcc ctcaccaatg aatttgttgc ttatgccgaa aggctctatt
     2161 tgcaacagca ggatttgatg aaaaaagcag atctgaatat tgaaaaagca aaaaaattac
     2221 aggctcagct acgaaagtcc taccaggatg cggaaaattc gacttttact ttcagggagt
     2281 tctctccggc gggagcttct cctgcacccg ggccccgttc ttccacagca tcaaaaggac
     2341 agggtaaagg gttatgggta ttagtgaaaa cgattgagta tgatggtaaa actgaactgg
     2401 atcacacaaa taaaaacggc gtttaccggg taagtggaaa cagttcagcc ggtgctttta
     2461 tctatacatg gaaatacctt ggcaaagccg ataactatta taacccgcca ttgctgagcg
     2521 atgaaaattc aactattaaa tgcaacgtga gcgcgcctcc taaagtaatg gacgccggtg
     2581 aaattatatc cctgaaaatt tcaatgtcct ttgctgctca aaagctttcc tatttcactt
     2641 ctggtgcaac tgtttcagca gattttgaca agccggattt aggcattggg tttgccagtg
     2701 gctcaaatat tagttttgta aataatgccg ggaaatcttc atttaaaatc aatacacaca
     2761 aatcggttaa gatttatact gtagatgaaa tagtaacggc gaccgcgcct gcaggagtta
     2821 aagatatgcg tattgcaata agagcatcat tcaatccggg cgcaaaaatg gggacttgtt
     2881 atatttatga atggaaatac gcctactgat tttttgtaaa tggcagtaaa aattcttaaa
     2941 ccattcataa aaaatggtat tgaaatttag tgtttctatt ctctgtaact aaattatttt
     3001 gaaacagctg ggatttgtgc atgttattca aaaaataccc ttcccgtggt atggccggtt
     3061 ccgcgtacaa caatatcttt atttgtatga aaaagaatct gctctgcctg ttccttttat
     3121 gtaccacagt attccacggc ctttcccagg ataaaatggc tttattggtg gccattggcc
     3181 agtatccccc tgccagcagg atccggccga ttgcgtctgt tacggatctg aaatacctca
     3241 ggaccgcgct gaataaacac ggcttcgcgg ataagaacat tcagacattg atcaatgcca
     3301 aagccaccaa acagggaata ctcaccagtc tgactgccat ggcccgtaaa gcaaagaaga
     3361 acgatattat cgtactcagt tttggttgtc acgggcagca gatccgcgac cagcgtacgg
     3421 tagaactggg aaaagatgaa gatgatgggt atgatgaagc cctggttccc tatgatgcga
     3481 aaggcaccta cagcccaacc ggctaccggg gcgaaaaaca cctgcgggat gatgatctct
     3541 atccgatatt gatggagata cgactgaaaa tcggtaagga cggcagtttg ctggtgctga
     3601 tcgatgcctg tcattcgggt accggtaccc gggccgatga ttttccggct tcgagaggag
     3661 aaccggttcc ttttcctgat ccggaaaatc catataacgc agccgatatt tcagatctcg
     3721 ataaaaaaga aacttttctg gacatacagc ccgatagtct ttccaatatg gtggtgatca
     3781 gcggctcggg tccgcaccag atcaataaac aggtcctggt aaacaaggaa gaagtgggtt
     3841 ctctttccta cggtttttat aaggccatga gcgaattgac agccggtcac gattatgcgg
     3901 ttttatatga aaaaatcaaa tcttccatac aggccattat ccccgatcag ttgccgatga
     3961 tagaaggcaa taccagccag gaaattttca gtggccgcta tttgcctgtt cgtgagagga
     4021 atattatccg ggtcggcttg aaagaagctg cttccaattc ttccgattcc gttttcacgt
     4081 tgaatcgtgg aatgatggat caattgtccg ttggggtaac aggtaccatt tacccgcttg
     4141 gtaaagaaac accggttgcc accgctgtta tccggaaaac cagccatttc agcagtgtag
     4201 gggtggcttc cgttttatta aagaaatcag agacctacga attaagatta acagaagcga
     4261 attacggtaa cctgtcagct ttgttgcaat ttgacaagca ggataagttg ccttccggaa
     4321 cggagaaaca ggtccgtaaa cttttatctc cctatgccta tatttcattc agtgatcagc
     4381 gggctgattt tcagcttcgc caatcagcaa ccgccactgg ccgtcaggtg gaacttaccg
     4441 accggaacaa tcagttagtc tggtccgctc ctcttacccc ggcagataca gtcgcggctg
     4501 ccgatcagct tatcctgatc cggggaataa ggaatgcctt gcaggtaaag tatctccgga
     4561 caatgcctga tgggggtgaa ttgtctcaat gggttacggc ggaaatactg gcgaacaacg
     4621 gggggaggac cgccctggaa ttcaccgaag gcgaccagta tacattgaaa ctggtaaata
     4681 acagtgagta taaattattc tataccgtgc tggatattta tccggataat aaagtggacg
     4741 tgctttatcc atacaaaggg aaagaaccgg ccgattacag tatggagaaa aagaatcctg
     4801 tagaacggaa gttgaccgtg agcaaaggct cacccaccgg tgtggagttc ctgaagatca
     4861 ttgtttcaaa agagccgatg gacctgcgag gggtctttga acaaaagatt cagcgggata
     4921 atatgatgtc tttccaggtg atgctggatg atcttttcag ccggcctgaa gggggagaaa
     4981 cagtcagggc ggatgtatcc gcgattaaaa tggaagagat tggcattatt acggttaatt
     5041 tcatgatcag gaaataatcc aggtatgcaa ctgcttaaaa aaacaggcct cagcttatta
     5101 ttcatcttca ctgtggtgtt caccatcgcg caggaaatcc caaggacaga agccagggtt
     5161 gtatggctgc ccatcgaccg gacatatcag gataaggatt cactgattgc gttcctgtca
     5221 ggagtaagca acatcgggtt gcagaaccgg caaatactga aagggtatca gtcatcggtt
     5281 gatgaaatac ctggtattcg gccaaagaaa gacttcatgg agacaggtgc gggattcatt
     5341 tattcaacag acagtatcca ggtcgcattt atccggatgt ataatccggc tgatacctta
     5401 ggcaggggcg acatgattag tctgcagatg caggtgcctc aattacctta ccggggtgtt
     5461 tattctgaac tggcatttaa cgggatcata tttacagatg cggaaaaaaa tccattgtat
     5521 accttggatt acctctttca tcatgatagc cggaagctgg aggattcgct ttttggggtc
     5581 attataaaaa gtttgcatag tacctatgaa ctggtgaagg accgtccgga attacctgct
     5641 tccctgctgg ctaaggttac agccggtcgt tttaaaaaca ggattcccct ggagattata
     5701 cgggacgtaa cccgcaagga cctcgaagcc tttttcatgt atacacaagc ttacccggtt
     5761 gggtatatgg ggaagaatta ccgggccagc gaaagttttg ccggatggct gatcagcaac
     5821 agtccgtata gtgtgggcga agtaaaacaa ggactatttc cggtgtataa aaatcctgtc
     5881 gagtttaaga aaaggctgtc tgaatataaa accgatgtgc tgaatgaaca tacagcaacc
     5941 tccctggcta ctgatgccat cggattatcc aatcagctta aattcacaga agcccagcag
     6001 ttatccgatt tctccattgc tttggcagag ggggtaaacg atacaatgtc aaagccaacc
     6061 gtttatctct gtaaggccca gctcttcctc gatcaggaaa agtatccgga gtcagttacc
     6121 tggtgtgata aagcgattaa agccgcattg cttgccggtg accgggatat tgaaattcag
     6181 gccatcatca agaaagggta ttgctattac aaattgtcaa agtaccaggg tacggaaacg
     6241 atgatgagta atgcggttga taaactgaac cagcaccggg caacactggg agagaaagta
     6301 tataataata attaccggaa aatactggag taccggtcct cgatccggaa taatgccggc
     6361 cgttatgacg aagcgctgca attactggat tcagcgatcc gtattaacaa ccggatcaat
     6421 agttatgatg ctcagctggc caatgccggg tattatgctt ttataggcag ggtgtataac
     6481 gatcagggta aaccggcaga tgctgtacct gcattgaagc agtcggtcac tatttacaga
     6541 aataacagtg atgtgttaaa cagcgcgatt gttgaaaacg acctcgctta cagctactac
     6601 aaacagggaa attatcgcca gagcctggag caggccggta tggctttaaa taaattagta
     6661 agacttgatg atttcaataa tgcggggtat tccatgtcgc tcacaggttc ctgttactgg
     6721 caaatgggat tatacgactc cgccttgtct gcgcatcatg catcactggc cttgcgggaa
     6781 aagagtggca atctgagtgg acaggcttat tcatggacaa gacttggcga tttatataaa
     6841 gaaagcggat ctaaaaagca atcccttctt gcttatgaga aagcggctga tttatacagg
     6901 aaactgatgg acagtacggg gttggccgaa gtatttaatg ccagggggca agtgtacctt
     6961 gatgatgaaa attttaagaa tgccgccagt gaatttgaaa aggccaatgg ggttagtcat
     7021 aagtctacgg tggaagcatt gtataagctc ggtattgcct ggagcagtat tgatacagta
     7081 aaagccagaa agtactttca ggaagcaaga cggaatagca ttgacgatgg gaataccggg
     7141 tatcagtttg cttccagcag gttattggcg aggatggcgt atttaaacca ggacaagcgg
     7201 ctgggagaac gttattataa cgaatgtgtg gcactgggta aagaaataaa gacggctttt
     7261 gcggatgcac agtgtctgtc actaacggcc tataaatttg aatgtgagac gcaacttgac
     7321 agtgccttat tttattaccg gaaagcaatg acaatcaccg atacggttga ccggggtgtt
     7381 tcagtggggc acctgaatag tattgccagt atatttattt ctatgggtga attccggaaa
     7441 gcggaggacg cactcaacag cgctatccgc gtggccaggg atatttcaga cagtctgtcg
     7501 ttgggcagta cgctgcagtt cagttcattt ctttattcaa gaacagcgga gtttgcccgt
     7561 ggcctggcaa acaatgacag cgccatcgcc attttcaaaa actcaggaca cctgatccgc
     7621 ctggccggta cgtatgcatc aagggcaaca ttatacagca gtatggggga gaataaggaa
     7681 tccatcctgg caaacctgta cgcggatagt ctctataatg aggagatgca gaaagagcaa
     7741 caaggtctgc tatataacaa tatcggaatt gtctatatta gtcagggcga ttacccaacg
     7801 gccatgaaat acctgctgaa atcgctgacc atgctgccta aagtgactaa caatgaggat
     7861 tacctgctgg tacaggggaa tatggcagag tgttatatcg ggttaaagaa atacaaggaa
     7921 gcgagaacct tgctgatgga gttcctgccc ctgactcgta aaatgaaatt gaaccggata
     7981 gcttccggga tggcactggt tttcggaaaa ctcagtctgc aggaaaacaa gatagccgac
     8041 gcgacccggt attatactga agcctggctg ttcggtgaag ccagtggaga aaaggaaaaa
     8101 gcgttagagg cattaatcaa cctgggccgg atataccgca aagaaggaaa taaggatacc
     8161 gcagccgccc gtattcgtga agcggtggcc ctgtctgtga aatacaaact ggctggcggc
     8221 tgggaagcat tttatgaatc gggactgttt tcatacgaag caaagcaata tgacaccgcg
     8281 gtggtttatt tcaaggaggc cgtcgggttg ctggaaacgc tgacggataa actgtttggc
     8341 ggagaggcgg ccaaaaagat atttaataat gatccccgta aatcggattt atttaataag
     8401 ataactttct cgtattataa cctgggagat attaatcagg catggtccta tgccaatcgg
     8461 agtaatatag ccggtatccg tgaattgtcc ggatcgctga ctacgaattc cagtgatgcc
     8521 gaaaaaaatg aggcgttgaa gaaattattt tccctccagg aatcaaaaaa ggcattggaa
     8581 ggaactctgg aaaagcagga aggggtagcc cggcaggaaa cgctgaagaa aatagaaata
     8641 ctcgaagcgg attacaacaa ctttttatca gatgtggtgg agctgtaccc ggacctgggt
     8701 acctatttca gtaaatcaaa tgcggatgaa ttcaataagt ataaatcaaa actgcctgcg
     8761 gatgt
//
LOCUS       DAOPMW010000107         8763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8860_length_8763_cov_7.867938, whole genome shotgun sequence.
ACCESSION   DAOPMW010000107 DAOPMW010000000
VERSION     DAOPMW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8860_length_8763_cov_7.867938"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..1310
                     /locus_tag="PLU11_06885"
     CDS             435..1310
                     /locus_tag="PLU11_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPN58879.1"
                     /translation="MLKSMTGFGRAEKNVADKTFLVDIKSLNGKQFELQLKIPAFLKP
                     FEFDIRRLLSEKLGRGSVDCIISLKDTGTAKPVTINTDLAKAYYQPLAELSKALNLDA
                     SNILSTLVKLPEVITPSSDTLTDAEWNQFQSVLETAIGNLNTHRLDEGKSLEDDLTAR
                     INNIGKLQEEVISLEPLRKQKIREGITRLLEENVGKENYDGNRLEQELIYYIEKIDIS
                     EEQVRLKNHLDYFKDLLADPDDSKGKKLSFILQEIGREINTTGSKAYDSTIQKCVVQM
                     KDELEKAKEQVLNVL"
     gene            1348..1833
                     /locus_tag="PLU11_06890"
     CDS             1348..1833
                     /locus_tag="PLU11_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPN58880.1"
                     /translation="MKPLQSFAFIVLLLGTFSCTQTDLFEKTAVIPEHSWKSNFKPSF
                     SFTIKDTSSPYMVYLIIRHNDRYSFNNIYVNLFAKGPGQDSTQGVTYDIRLGTDESGW
                     LGSGMDDIYEHRVPLTPRSSEQAFYFRKPGEYTFTIEQIMRQDPLENVLNAGLRIEKK
                     K"
     gene            1841..2830
                     /locus_tag="PLU11_06895"
     CDS             1841..2830
                     /locus_tag="PLU11_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58881.1"
                     /translation="MQDANRKGLKRTTFIYFTLLFYVLAALVWWFISLEKQSKEIARQ
                     QYENLSVRSGSLSVLKLADEMQSIVKEEKRNTRKYIAEGITFLILIVIGAILVYRSIR
                     KQFNLQQQQQNFMMAVTHELKTPIAVARLNLETLQKYTLDPEKQKKLIRNTLDETTRL
                     HLLTNNILVSSQLDSDRYHSAMEELDLSTLLKDCMRDFRKRFPDRVFEEQIEEEADIQ
                     GDPLLLQMLINNLVENAIKYSPKEGKIKAVLAKASGSIRLQIIDEGPGIPEEEKTRVF
                     SKFYRIGNESTRKAQGTGLGLYLCRKIARDHNGDIAVTNNLPTGSNFAVIFKT"
     gene            2827..3534
                     /locus_tag="PLU11_06900"
     CDS             2827..3534
                     /locus_tag="PLU11_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN58882.1"
                     /translation="MSGDKKTSILLVEDEENLHEALKLNLELEGYEVTSAFDGAAALK
                     AVSNEYFDLIILDVMLPEMDGITVTETIRLSNNDVPVLILSAKNSSADRVLGLKKGAD
                     DYLTKPFNLEELLLRVYKLINKNKKLQDKTTLGDHYEFGNNQVDFKAQEATSFNGEKI
                     QLSKKETMLLKLLIENKNEVVPREKILQSVWGYNVYPTTRTIDNFILNFRKYFEEDSR
                     NPRYFHSVRGVGYKYSE"
     gene            complement(3526..5295)
                     /locus_tag="PLU11_06905"
     CDS             complement(3526..5295)
                     /locus_tag="PLU11_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPN58883.1"
                     /translation="MKKTKAIFFLLLISLTGSSQVVFKTIAPTEPVVAGESFRIQFYV
                     EGSPAHELIPPDFYPFRLVEGPDIYPGKSKGTGLLFKNYIYTLVAAQPGEYRIPGASL
                     RTGNSILQSSNARITVISAARSMESVNTANGPTGYYLGPEENAQIKIKENLFLRVSAD
                     KKTCYVGEPVLVIYKLYSRLESSTDIIKNPGFYGFTVQDMIGLQEQKLSTEQLNGKEF
                     YVHIIRKLQLYPLQAGRFTIDPMEIKNRVKFSTRVMNRKTEQRIAEGMLGASEEEPEE
                     EGVTIVENQLRTEPLSIEVKPLPEKNKPVDFNAAVGKFTLQTRLEQPTLSRNEEGFLL
                     VTIEGEGNFIQLSAPVIEWPAGMEGFGAVVTDSLDKLKIPLAGKRQFRFGFVCTEPGA
                     YKLPVVRFSFFDTDSNRYSSLVSKVLEVNISNQEKKRLQNEEHKISIDELSQQKSRIA
                     IIVVFLLVSSVLAYWALKKKPAAGIPVPVKKVKPDISEVLSDAAGMVAGEDNAFYATL
                     HRSIWNYFQDYFELAGSARNKENLFDLLKQKGAGDDAIHSLSTIFSDCETGIYTSAVT
                     GKSNAVLLEETRELLEKMNGILF"
     gene            complement(5369..6085)
                     /locus_tag="PLU11_06910"
     CDS             complement(5369..6085)
                     /locus_tag="PLU11_06910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN58884.1"
                     /translation="MERNSQLNVVVTGASKGIGKAIAEIFAANGASLYLCSRGEGALY
                     KTMEELQTRYPQAIVKARPADLRVKEETIAFGNWCLSQAIPDILVNNAGLFEPGSVHN
                     EPEGVLESQLNINLFSAYHLTRSLLPAMMNRKQGHIFNMCSIASLHAYANGGAYSISK
                     FAMYGFSKNLREELKPHGIKVTSVHPGAVLTNSWGDFDNSSNRIMEAADIAKMVWAAS
                     SLSVQACVEDILIRPQLGDL"
     gene            6123..6863
                     /locus_tag="PLU11_06915"
     CDS             6123..6863
                     /locus_tag="PLU11_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPN58885.1"
                     /translation="MNKEINSPGEKRYTHLFFDLDHTLWDFEANSRVTLLDLFNDLQL
                     GERGVHDFDLFHKNYLVHNDKLWDRYRNGYIKVDELRWKRMWLTLLDFKIADEPLARK
                     MGVLFLDLLPTRNLLFPYTLEILDYLQAKQYQLHMITNGFEKTQYSKLENSGLDKYFT
                     HVITSEGSNSLKPHKEIFDFAFQKAGADPAGSIMIGDTLEVDILGGMNAGIDQVHVNH
                     LNKNREAVSNQQLPTYTVFSLKELETIF"
     gene            complement(6868..7524)
                     /locus_tag="PLU11_06920"
     CDS             complement(6868..7524)
                     /locus_tag="PLU11_06920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPN58886.1"
                     /translation="MTIHKEGYKTIAIATTVFAIINLVSFYFISAGMPLVSLLLFILT
                     LGLLLFLISFFRIPNRQLTIQENAIVAPADGKVVVIEEVKADEYFNDRRIQVSIFMSP
                     LNVHVNRNPISGEVMYSQYHKGKYLVAWHPKSSTENERHSVVYRKDGREILVKQIAGA
                     LAKRIVNYLKAGQTVKQTDEMGFIKLGSRVDLLLPLDAKIHVKLGDKPQGGVTVIASW
                     "
     gene            complement(7721..8761)
                     /gene="dusB"
                     /locus_tag="PLU11_06925"
     CDS             complement(7721..8761)
                     /gene="dusB"
                     /locus_tag="PLU11_06925"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPN58887.1"
                     /translation="MVKIGNIQLPEFPLLLAPMEDVSDPPFRAVCKDNGADLMYTEFI
                     SSEGLIRDAIKSRKKLDIFEYERPVGIQIFGGDEESLAMAARIVEVTNPDLLDINFGC
                     PVKKVALKGAGAGVLKDIDLMVRLTSAVVKSTSLPVTVKTRLGWDDNTKNIEEVAERL
                     QDVGIKALAIHGRTRTQMYKGEADWTLIGKVKNNPRIHIPIFGNGDIDSPEKAVEYKN
                     RYGVDGIMIGRAAIGYPWIFNEIKHFVKTGTHLPSPTIQDRVAVARKHLYKSVEWKNP
                     VVGINEMRRHYTNYLKGLPNIKEYRGKLVTLKTVEEIDEVLNEIERVYAGHQFEKTPI
                     ELIDYHQNCPVN"
BASE COUNT         2403 a         2098 c         1948 g         2314 t
ORIGIN      
        1 tgttgcataa aaaaaacaat acgcctgaaa cccgctgttc atgcatgttt caggcaatcc
       61 tgagttttgt gtaaagaaat attttctatg tcgtcgatcg aaatgcattg atcaggattg
      121 tctgtgaatg atgaaaaatg cttcggttta tccacagctg attttaaaat gttaataaaa
      181 tagtttggaa attttttttt gttatatgaa ataattttta atattgcata gggaatgcaa
      241 attccctgta cttactaacc catccatata ttatccctcc gcttactacg gagggatttt
      301 ttttagcttt gtatcaataa aaaacagact tcttatactt ttccggttca atcattatct
      361 ctgctcaccg ttttttaccc ggctgattaa tcaataataa aaaatacctt tacaggaatc
      421 ataaatcccg ttccatgctt aaatcaatga ccggttttgg aagagcagaa aagaatgtag
      481 ctgacaagac cttcctggtg gatattaaat cgctcaatgg aaaacagttt gaattgcagt
      541 tgaaaatccc cgctttcctg aaaccctttg aatttgacat ccgtcgactc ttgtcggaaa
      601 aactgggaag gggtagtgtg gattgcatca tcagtctgaa ggataccggc accgccaaac
      661 ccgttactat taacaccgac ctggccaagg cctattatca gcccctcgcc gaattatcca
      721 aagccctcaa cctggatgct tcgaatatcc tgagcaccct ggttaaatta ccggaagtga
      781 tcacacccag cagcgatacc ctcaccgatg cggaatggaa ccaattccag tcggtactgg
      841 aaaccgctat cggcaacctg aacacacatc gcctggatga aggcaaatcc cttgaagatg
      901 acctgaccgc ccggattaat aatatcggca agttgcagga agaagtgatc agcctggaac
      961 ctctccgtaa acaaaaaatc cgcgaaggca ttacccggct cctggaagaa aatgtgggca
     1021 aggaaaatta tgacggcaac cggctggagc aggaacttat ctattatatt gaaaagatcg
     1081 atatcagcga agagcaggtg cggttgaaaa atcacctgga ctatttcaag gacctgctgg
     1141 ctgatccgga cgattccaaa ggaaagaaac tctcctttat cctccaggaa atcggccggg
     1201 agatcaacac caccggttcc aaagcctatg actccaccat ccagaaatgt gtggtgcaga
     1261 tgaaagatga actggaaaag gcaaaagaac aggtactgaa tgtactctga aagttgtgtt
     1321 ctgtttattt ttgtaaaaac cgtttatttg aaacccctac agtcattcgc ctttatcgtc
     1381 ctgttgctcg gaaccttttc ctgcacgcag actgacctgt ttgaaaaaac agccgtgatt
     1441 ccggaacata gctggaagag taatttcaaa ccttctttca gttttaccat taaggatacc
     1501 agttcgccct acatggtcta tctgatcatc cggcacaatg accgctatag tttcaacaat
     1561 atttatgtga acctgtttgc caaggggccc ggacaggatt cgactcaggg cgttacttac
     1621 gatatccgcc tgggtaccga cgaaagcggc tggctgggga gtggaatgga tgatatttat
     1681 gagcaccggg taccgcttac gccaagatca tctgaacaag ctttttattt ccgaaaaccc
     1741 ggagaatata cgttcactat tgaacagatc atgcgccagg atcccctgga aaatgtgctg
     1801 aacgccggat tacggattga aaagaaaaaa tagtttcatc atgcaggatg cgaaccgaaa
     1861 aggcctgaaa cgcaccacgt tcatttattt caccctgctg ttctatgtat tggccgcact
     1921 ggtctggtgg tttatctccc tcgaaaaaca aagcaaggaa attgcccggc agcaatatga
     1981 aaacctgtca gtccggtctg gttcgctgag tgtattgaaa ctggcggacg aaatgcaatc
     2041 tatcgtcaaa gaagaaaaac gcaatacccg gaagtatata gccgaaggga tcaccttcct
     2101 gatcctgatt gtgataggcg ccatcctggt ctaccggtcg atccggaaac aattcaacct
     2161 gcagcaacag caacagaatt tcatgatggc cgtcacgcat gaactcaaga ccccgattgc
     2221 cgtagccagg ctgaacctgg agactttgca gaaatatacc cttgatccgg agaaacagaa
     2281 aaagctgatc cggaacacgc tcgatgaaac cacccggctt cacctgctga ctaataatat
     2341 cctggtttcc tcacagcttg actccgaccg gtatcattct gctatggaag aactggacct
     2401 ttccaccttg ctgaaagatt gtatgcgtga tttcaggaaa cggttcccgg accgcgtgtt
     2461 tgaagaacag atcgaagaag aagcagatat ccagggagat ccccttttat tacagatgct
     2521 gatcaataac ctggtggaaa atgccatcaa gtattccccg aaagaaggaa agatcaaagc
     2581 cgtacttgcc aaagcctccg ggtcgatccg gctccagatc attgacgaag gaccgggcat
     2641 accggaagag gagaagaccc gtgtcttttc caaattttac cggattggta acgaatctac
     2701 ccgaaaagcg cagggaaccg gcctgggact gtacctttgc cgtaagattg cccgcgacca
     2761 taatggcgac attgcggtga caaataatct gcccacaggc agtaattttg cagttatttt
     2821 taagacatga gcggagataa aaaaacatcc atcctgctgg tggaagatga agagaatctt
     2881 catgaagcgt taaagctgaa cctggaactg gaaggctatg aagtcacttc tgctttcgac
     2941 ggggccgctg cgctgaaagc ggtcagcaat gagtattttg acctgatcat tttagatgtc
     3001 atgttgcctg aaatggacgg catcaccgtt accgaaacga tccgcctcag caataatgat
     3061 gtaccggtac tgatcctcag tgccaaaaac agcagtgcag accgggtact gggattgaaa
     3121 aaaggagccg atgattatct caccaaacct tttaacctgg aagaactcct gctccgggtt
     3181 tataaactga ttaataagaa caaaaaactg caggacaaaa ccaccctggg agatcactat
     3241 gagtttggca ataaccaggt ggattttaaa gcacaggaag ccaccagttt taacggggag
     3301 aaaatacaac tgagcaaaaa ggaaaccatg ctgctcaaac tgctcattga aaataaaaat
     3361 gaagtagtgc cgcgcgagaa gatcctgcaa tccgtttggg gctataatgt gtaccccacc
     3421 acccgcacca ttgataattt catcctgaac ttccggaaat atttcgagga agacagccgt
     3481 aatccccggt actttcattc tgtacgtggc gtaggttaca agtattcaga ataagatccc
     3541 gttcattttc tccagcaact cccgggtctc ttccagcaat accgcattgg atttgcctgt
     3601 caccgcactg gtgtagatgc ccgtttcaca atcggaaaag atggtactga gtgaatgaat
     3661 cgcatcgtca cccgcccctt tttgtttcaa caaatcaaac aggttttctt tattccgtgc
     3721 actgccggcg agctcaaaat aatcctgaaa atagttccag atagaacggt gcagtgtggc
     3781 ataaaaagcg ttgtcttctc cggctaccat tccggcagca tcagacaata cttcactgat
     3841 atcgggcttt acttttttta ccgggacggg tattcccgct gctggttttt tcttcaatgc
     3901 ccaataagcc agtaccgagg ataccagcag gaaaaccaca atgatggcaa tgcggctttt
     3961 ctgctgactc agttcatcaa tcgaaatctt atgttcttcg ttctgtaacc gcttcttctc
     4021 ctggttactg atatttactt ccagcacttt acttaccagg ctactgtacc ggttactatc
     4081 cgtatcaaaa aaagaaaaac ggacaacggg caatttatag gcaccgggct cagtacaaac
     4141 aaatccaaac ctgaactgtc gcttgccagc cagggggatc tttaatttgt ccagtgaatc
     4201 agtcaccacg gcgccaaaac cttccatacc ggccggccat tcgataacgg gtgcagagag
     4261 ttgtataaaa tttccttccc cttcaatcgt taccagcagg aacccttctt cattcctgga
     4321 cagggtgggt tgctccagcc gggtttgtaa agtaaacttc ccgaccgccg cattaaaatc
     4381 cacgggttta tttttttccg ggagcggttt cacttctatt gacaggggct cggtacgcag
     4441 ctggttctct acgatggtga ctccttcctc ctccggctct tcttcactgg cacccaacat
     4501 cccttccgca atccgttgtt ctgttttgcg gttcataacc cgggttgaaa atttcaccct
     4561 gttttttatt tccatcggat caatcgtgaa acgtccggcc tgtaaaggat atagctgaag
     4621 tttacggatg atatgtacat aaaactcttt cccattcaat tgttcggtgc tgagtttttg
     4681 ttcctgcagc ccaatcatat cttgtacggt aaatccataa aaaccggggt tcttgatgat
     4741 atcggtactg gattctaacc tggaatataa tttatagatc acgagtaccg gctcgcctac
     4801 ataacaggtc tttttatcag cggacacccg caaaaaaaga ttctctttga tttttatctg
     4861 tgcattttcc tcaggtccga gataataacc ggtgggaccg tttgccgtat tcaccgattc
     4921 catggaacgg gcagcggata taacagtaat acgggcgtta ctactttgca aaatactgtt
     4981 gccggtacgc aggctcgcac cgggaatccg gtattcacca ggttgtgcag ccaccagggt
     5041 gtatatataa tttttgaaaa gtagtccggt gccttttgat ttaccaggat aaatgtccgg
     5101 accctctacc aggcggaatg gataaaaatc gggcggaatg agttcatgtg ccggtgatcc
     5161 ttccacataa aactggatgc ggaacgattc cccggccaca accggttcgg tgggggcaat
     5221 agtcttaaag acaacctggg atgaccccgt aagtgaaatc agcagaagga aaaatatagc
     5281 tttggttttt ttcatgtaag caggtctaaa agtaaagccc gactttcaat aaaaaaccta
     5341 aaaaatggtt aaacggcccg gagcgctgtt acagatcgcc cagttgaggg cggatcaaga
     5401 tatcctccac acaggcctgg acggataaag aagaggccgc ccagaccatt ttggcaatat
     5461 cggcagcttc catgatgcgg ttggatgaat tatcaaaatc accccaggaa ttggtcagta
     5521 cggctccggg atgaacagac gtaaccttga taccatgtgg tttgagttcc tcccgcaggt
     5581 ttttactaaa gccatacatt gcaaacttgc tgatactata cgctcctcca ttggcgtagg
     5641 catgcaggga ggcgatagag cacatattga aaatatggcc ctgtttacgg ttcatcatgg
     5701 ccggcagcag actgcgggta aggtgatagg cactgaagag gtttatgttg agttgactct
     5761 ccagtactcc ctcaggttca ttgtgtacac tcccgggttc aaacaagccg gcattattga
     5821 ccagtatatc cggtatggcc tgtgacaggc accagttccc aaaagcaatc gtttcctctt
     5881 ttacccgtaa atcggccgga cgtgctttca cgatggcctg cggataacgg gtttgcaatt
     5941 cctccatggt tttataaagg gccccttctc ccctggaaca gaggtaaaga ctggcgccat
     6001 tcgctgcaaa tatttcggct attgctttcc cgattccttt cgaagcaccg gtcaccacta
     6061 cgttgagttg actattcctt tccatacctg tctgaattgt tctgtaaatt gcaaacttat
     6121 ctatgaataa agaaattaat tcccccggcg aaaagcgtta tactcacctg ttctttgacc
     6181 tggaccatac gctctgggat tttgaagcca acagccgggt caccctgctg gatttgttta
     6241 acgatctgca actcggtgaa cgcggggtgc atgactttga cctgtttcac aaaaactacc
     6301 tggtgcataa tgataagttg tgggaccggt accgaaacgg atatatcaaa gtggatgaac
     6361 tgcgctggaa aagaatgtgg ctgaccttgc tggattttaa gattgccgat gaaccactgg
     6421 cccgtaagat gggcgtcctg tttctcgacc tgctgcccac ccgtaattta ttattcccgt
     6481 ataccctcga aatactcgat tatctgcagg caaagcaata ccagctgcat atgatcacca
     6541 acggttttga gaaaacacag tacagtaaac tggagaattc cgggctggat aaatatttca
     6601 cccatgtgat cacctccgaa ggaagtaaca gcctcaaacc ccacaaagaa atttttgatt
     6661 ttgcttttca gaaagccggc gccgatccgg ccggcagcat catgatcggg gatacactcg
     6721 aagtggatat actgggggga atgaatgcgg gaatcgatca ggtgcatgtc aatcacctta
     6781 ataaaaaccg ggaagcagtc agcaatcagc agctgccgac ctacaccgtg ttctccttaa
     6841 aagaattgga aacgatcttc tgatctatta ccaggaagca atcaccgtta ccccgccctg
     6901 tggtttatca cccagtttta catggatttt tgcatccagt ggcaggagca aatcaacgcg
     6961 tgagcccagt ttgataaaac ccatttcatc cgtttgcttt acggtctggc ccgctttgag
     7021 gtaatttaca atccgtttgg ccaaggcacc cgcaatttgt ttcaccagga tttcccggcc
     7081 atctttgcgg taaaccacgg aatgccgctc attttccgtt gacgattttg gatgccaggc
     7141 cacgaggtat tttcctttgt ggtactgact gtacatgact tcaccgctga tgggattgcg
     7201 gtttacgtgt acattgaggg gactcataaa aatggagacc tgtatacgtc ggtcattaaa
     7261 atattcatcg gccttcactt cttcaatcac caccactttc ccatcggccg gggctacaat
     7321 cgcgttttcc tgtatcgtga gctggcggtt tggtatccgg aaaaaagaga tcaggaataa
     7381 cagcaaacct aatgtcaaaa taaaaaggag caggcttact aacggcatcc cggcacttat
     7441 aaaataaaaa gataccaggt tgataatcgc gaaaacagta gtagcaattg cgatggtttt
     7501 gtagccttct ttgtggatcg tcatggagga aactttatgg agcaaatata ggataatcag
     7561 ttgtcagatg tcggttgtca ggaaacagcc cgctaagcta gaagcaagaa gctagatgct
     7621 agaaggaaac agcccctcct tagaaggttt aaactaaaac aacgaatcgt tactcgcttt
     7681 acggcatcac gctcgtactt cgtacttcta acttcgtagt ttagttgacc gggcagttct
     7741 gatgataatc gatcagttcg atcggggttt tttcaaactg gtgaccggca tagacccgtt
     7801 cgatctcgtt cagcacttca tcgatttctt ccacagtttt gagtgtaacc agtttgcccc
     7861 ggtattcttt aatgttcgga agacctttga ggtaattggt ataatgacgg cgcatttcat
     7921 taatgcccac cacggggttc ttccattcaa cagatttgta taaatgtttg cgggccacgg
     7981 ccacacggtc ttgtattgtt ggagaaggca ggtgagtacc ggtctttaca aaatgtttga
     8041 tctcattgaa gatccacgga taaccgattg cggcgcggcc gatcatgatg ccatccacgc
     8101 cataacggtt tttatactcc acggctttct cgggtgaatc aatatcgccg ttcccgaaaa
     8161 tagggatatg tatccttgga ttatttttca ctttgccaat cagcgtccag tcagcttccc
     8221 ctttgtacat ctgtgtacgg gtacgtccat ggatcgccag tgccttgatc cccacatcct
     8281 gtaaacgttc ggctacttct tcaatatttt ttgtgttatc atcccaaccc aggcgggtct
     8341 ttacggtaac cggtaatgat gtagacttta ctactgctga agtaagtcgc accatcaggt
     8401 cgatatcttt cagcaccccg gcaccggccc ctttcagtgc tacttttttt acagggcacc
     8461 caaaattgat atccagcaga tcgggattgg taacttccac aattctggcc gccatggcca
     8521 gactttcctc atcgccccca aaaatctgga tgcctaccgg tcgttcatat tcaaaaatgt
     8581 cgagcttttt ccggcttttg attgcatccc ggatcagtcc ttccgaagaa ataaactccg
     8641 tgtacatcag gtccgccccg ttatccttgc atacggcacg gaaaggggga tcactgacat
     8701 cctccatagg agccagaagc agcgggaatt caggaagttg tatgttgcca atttttacca
     8761 ttc
//
LOCUS       DAOPMW010000108         8716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_8910_length_8716_cov_6.962014, whole genome shotgun sequence.
ACCESSION   DAOPMW010000108 DAOPMW010000000
VERSION     DAOPMW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8910_length_8716_cov_6.962014"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLU11_06930"
     CDS             complement(<1..930)
                     /locus_tag="PLU11_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPN58888.1"
                     /translation="MALYGSNPLKRSPILQILIPFIIGILYQYYLSMPQWLVIILIPV
                     SLTGLLIFHRIPVLARFRHSMLSGIFTAILFFSLGAALCRNQDIRLQDNWYGHQLRDT
                     SSLLLVLEEPLVEKSKSFKAIASVRASINNNQVESVQGRLLIYFSKDSTVQSLDYGSR
                     LIIRQPVREIKSAGNPGAFDFKQYSLFQCITHQVYLRPVDYEVLPGKEQNWFRKQLFR
                     SGQFLVQLLKRYIPGRLESGIAEALLIGYKQDLDPEIVQSYSNTGVVHIIAISGLHLG
                     LIYWLLLLLLKPLNQSKKTRWLHATLVITGLWTF"
     gene            complement(984..1454)
                     /locus_tag="PLU11_06935"
     CDS             complement(984..1454)
                     /locus_tag="PLU11_06935"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN58889.1"
                     /translation="MKQALSDLLLRLENTLNGEPWFGRSALSLLKEVNPEGVFMKTGN
                     GGHSLADLLWHMITWAQFTLDRLEKKQEKDMTAFESLDWRTIDPAIHSWKNGIKEFTL
                     IYGKISDLLKKKNDAFLEEKVDYRDYNVQFLLNGLIEHTIYHLGQIAYAQKQLN"
     gene            complement(1464..3011)
                     /locus_tag="PLU11_06940"
     CDS             complement(1464..3011)
                     /locus_tag="PLU11_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58890.1"
                     /translation="MRAPSSFLWQLIQSLNRNERLYFKRNFKQAGAGVDRLYIRLFDA
                     IAAQKVYDEKVLLDKFSPVLNKKNIASQKNYLQKQVGDALVQYESIRDPGKDVYASIQ
                     LIRIYRKKGLYDEAFSIWKKAIELARSGEAFAMSNLLKTEFEKMVIFSPSHSDQDELL
                     DIFKKNIITYTEYAELITLRDIYTETLLLKKKTHFGQDKQLLDTIHTLLDRVNRIDMA
                     SQAYSFWYRHYYLMSKATLQYLLNDMAGAMELLKTHFSNWKKQDKFVATHGEFYIELL
                     YMINYAGILYGEYDYVTAVFNDPLNKQITDPSHRAYFEVTRYLALNKIYNKTARYEEV
                     KKLLVVMKANYTSWEPSLTDELNSTLSFSMGVGCFALEQYEDALFFTRRAITAFPEGS
                     RDEHNAVGQLLLLLITWCLNNARLFDAQYKATYQFFYKRKKKQVFETALKQCLHRCFY
                     MTEQKKKIQEFKKALQLLEENRNNPIQQRAFAIFNFEGWLQSRVMRISYRQYVEKKVR
                     AELKEAV"
     gene            complement(3316..6141)
                     /gene="carB"
                     /locus_tag="PLU11_06945"
     CDS             complement(3316..6141)
                     /gene="carB"
                     /locus_tag="PLU11_06945"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPN58891.1"
                     /translation="MPKDNSIKSVLIIGSGPIIIGQACEFDYSGTQAARSLREEGIKV
                     ILINSNPATIMTDPMMADRVYLLPLTVESIEQILQENQIDAVLPTMGGQTALNLAKEA
                     EDLGLWKQYNVRLIGVDIKAIDKAEDREKFRQWMIQLGVPVATAKTANSYLEGKEFAQ
                     QIGFPLVIRPSFTLGGTGGGFVHDKEDLDEALNRGLQASPIHEVLVEKAVLGWKEFEL
                     ELLRDSADNVCIICTVENFDPMGVHTGDSITVAPAMTLSDTGMQLMRNTAIMMMRDLG
                     NFAGGCNVQFAMSPDTEEIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LENQITKTTSAYFEPALDYVIVKIPRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTE
                     AIQKACQSLENNAVGLGYYGKSMMHAEELLEYIKTPKWDRIFRIKDALMAGISVGTIS
                     KATNGIDRWFLYEIQKICTIEKEIAKYDLDTLPIELLREAKVNGFSDEQISRILEHGD
                     EEDVYNKRKEAGITRVYKMVDTCAAEFEAKTPYFYSTFEGGNSNESKVSDKKKIIVLG
                     SGPNRIGQGIEFDYCCVHGLLAIKECGYEAIMVNCNPETVSTDFDMADKLYFEPVYWE
                     HLWEIVEHEKPYGVIVQLGGQTALKLAEKLTQKGIKIIGTSYDSLDIAEDRGRFSDML
                     KELEIPYPAYGTAWDVDEAIEVANKVGYPVLVRPSYVLGGQRMRIVINDDEVEKAVIS
                     LLKHIPGNKILVDHFLDRCQEAEIDAICDGEKFHVMGIMEHIEPAGIHSGDSNAVLPY
                     FNLTPLEVQDMVDYAKKIALKLNVKGLLNIQFAIKEGKVYVIEANPRASRTTPFIAKA
                     YNKPYLNYATKVMLGEMRVQDIPEEKFLDGFAIKEPVFSFNKFPGVNKELGPEMKSTG
                     EAIRFIKNLRDPYFRQLYKDRSMYLSK"
     gene            complement(6263..6811)
                     /locus_tag="PLU11_06950"
     CDS             complement(6263..6811)
                     /locus_tag="PLU11_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58892.1"
                     /translation="MTKLLWLLPLSFLLTACPFESNMPLEKAPVEPVDSSLLGFWYGI
                     VKDGSDYFGIEALEISRQSDSLYSVIRYGKAVKGDMILPDTSFYTAYSSRVRDQSFMN
                     LAALGPNGKPSKVWYLSAYSMSQDTLQVRTVTETFTTRKSFPRPEDFKKLVLDMSQNR
                     NIYDELFTLSYRKIPKPASFEP"
     gene            complement(6853..7905)
                     /locus_tag="PLU11_06955"
     CDS             complement(6853..7905)
                     /locus_tag="PLU11_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN58893.1"
                     /translation="MKLIIVIVFTAIYFTPVLACYNESHVNKSGKQTNRVEPMALFYN
                     EPDKAEAKSFLAKYNLTALKTYDKDIQSDIAVNLGYLGRYQEALDILKKLQAAYPEDY
                     TIAANLGTTYELLGKNELALQFIKKGLQLNPESHEGSEWVHVKILEAKIKMATAPDWI
                     YNNRVLGTGVKFDSKEAETLNDMAWDVEYQLQERVPFTPFPDALLANVFDELGDLYAT
                     QQSVELAYIAYDFALRNDSSDHFGVKSKMEALKPVLKKNKIPVPSWKEHYYNRKLDKI
                     QAEIKDEANEQVRNPEKVKDTTEGKTEITDRYPGRNKELEARNRRNNLILAGSIILAG
                     FAAIFIYTQLKKQKSR"
     gene            7990..>8716
                     /locus_tag="PLU11_06960"
     CDS             7990..>8716
                     /locus_tag="PLU11_06960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN58894.1"
                     /translation="MVKVFILFFFLVIQFVVSAQSGPVYPKGYFRNPVGIPLDLSANF
                     GELRSNHWHMGLDIRTQAKENLPVYAAAQGYIAKIGIRPQSFGRFIIINHPNGLSTLY
                     GHLNNFYPELEAWVREQQYAQESWAVELDIPKEKFPVSKGTFIAYSGNTGGSQGPHVH
                     FEIIDTKSSKRLNPLLFGFPIADNVPPTLIKLAVYDRSRSVYDQSPRFYPLKNTDSGY
                     IIPKLPVIETGLSRISFALQAYDR"
BASE COUNT         2157 a         2003 c         2017 g         2539 t
ORIGIN      
        1 gaaggtccag agtccggtta tgaccagtgt ggcatgtaac cagcgggttt tctttgattg
       61 gttcagtggt ttcagtaaaa gcaggagcaa ccaatagatt agtcccaggt gcagtccgga
      121 tatagcaatg atatgcacca cccctgtatt gctgtaggac tgcacgattt ccggatcgag
      181 gtcttgttta tagccaatca aaagtgcttc ggcgattcct gattccaggc gcccggggat
      241 ataccgcttc aacagctgca ccaggaattg tccggatctg aagagttgtt tcctgaacca
      301 gttttgttcc tttccaggca atacttcata atcaactggc cggagatata cctgatgcgt
      361 gatacactgg aaaagggagt actgtttaaa gtcaaaggcc ccgggattgc cagcactttt
      421 tatttcacgg accggctgac ggatgatcag cctggagcca taatcgagtg actgcaccgt
      481 actgtctttg ctgaaataaa taagcaatcg gccttgtact gattccacct gattattatt
      541 tatactggcc ctgaccgaag caatcgcttt aaatgatttt gatttttcaa ccaggggctc
      601 ttccagtacc aacaatagtg agctggtatc cctgagctgg tgaccatacc aattatcttg
      661 taaccggata tcctggttcc ggcataaggc tgctcccaat gaaaagaaga gtatagccgt
      721 gaaaataccg gatagcatgg agtgccggaa acgggccaat accggtatgc ggtggaatat
      781 gaggagtccg gtcagtgaaa caggaatcag gatgatcact aaccattggg gcatggacag
      841 gtaatattgg taaaggatac caataataaa aggaatcagg atttgcagga tgggactgcg
      901 tttcaggggg ttgctgccat agagagccat ggctcaaatt aatcaggctc ctgctgccag
      961 acaatactac tttacgggta tactcagttc agttgttttt gcgcataggc gatttgtccc
     1021 aggtggtaaa tcgtatgctc aataagaccg ttgagcagga attgtacatt gtagtcccgg
     1081 tagtcgactt tttcttccag gaaggcatcg ttttttttct ttagcaggtc actgatcttt
     1141 ccatagatca atgtaaattc ctttattccg tttttccagc tgtgtatcgc ggggtcaatg
     1201 gttcgccagt cgagtgattc aaaagcggtc atatcttttt cctgcttttt ctccagccgg
     1261 tccagggtaa actgagccca ggtaatcatg tgccagagca aatcggccag ggagtgtccg
     1321 ccgtttcctg tcttcatgaa aaccccttca ggattgactt ctttcagcag ggaaagggcg
     1381 gaacgcccaa accagggttc accatttaag gtattttcca gccggagaag gaggtcactg
     1441 agtgcttgtt tcatacccta aatttaaaca gcctctttca gttccgccct gacttttttc
     1501 tccacgtact gtctgtaaga gatccgcatc acccggctct gcagccagcc ctcaaaattg
     1561 aaaatggcaa aagcccgttg ctgaataggg ttgttcctgt tttcttccag caattgcagg
     1621 gcctttttga attcctgtat tttctttttt tgttcagtca tatagaagca ccggtgcaga
     1681 cactgtttca gggctgtttc aaatacctgc ttttttttgc gtttataaaa aaactggtaa
     1741 gtggctttgt actgagcatc aaacaggcgt gcgttattga ggcaccaggt tatcagcagg
     1801 agcagtaact gacctacggc attgtgttca tcccgcgaac cttccgggaa tgccgttata
     1861 gcacggcggg taaagaaaag cgcatcttca tattgttcca gtgcaaaaca gcccaccccc
     1921 atggaaaaac tcagggtgct gttgagttca tcagtcaggg agggctccca actggtataa
     1981 tttgccttca tcacaaccag caatttcttt acttcttcat agcgggcggt tttattatag
     2041 attttattga gggccaggta acgggttact tcaaaataag cccggtgaga aggatcggta
     2101 atctgtttat tcagcgggtc attgaagact gctgttacat aatcgtactc cccgtacaga
     2161 ataccggcat agttgatcat gtataaaagt tcaatgtaaa attccccatg ggtagctacg
     2221 aatttgtcct gctttttcca gtttgaaaaa tgtgttttca gtaactccat ggcaccggcc
     2281 atatcattca aaaggtattg cagggtggcc ttgctcatca ggtaatagtg ccggtaccag
     2341 aacgaatagg cctggctggc catatcgatc cggttgaccc ggtcaagcaa tgtatgaata
     2401 gtgtcgagca gctgcttatc ctgtccgaaa tgggttttct ttttcagcag taaggtctcg
     2461 gtataaatat cccgtaacgt aatcagctct gcatattccg tataggtgat tatatttttc
     2521 ttgaagatat ccagtaattc atcctggtcg gagtgggaag gactgaagat gaccattttt
     2581 tcaaactcgg ttttcagcag gtttgacatg gcaaacgctt ccccgctccg ggccagttca
     2641 attgcttttt tccagatcga gaaggcttca tcataaagtc cttttttacg gtagatgcga
     2701 atcagctgga tacttgcata cacatctttg cccggatccc ggattgattc atattgtacc
     2761 aacgcatctc ccacctgttt ctgcaggtag tttttttgtg aggcaatatt tttcttgttc
     2821 aggaccggac tgaatttatc cagcaatacc ttttcatcat atactttctg cgcagctatt
     2881 gcatcaaata accggatata aagccggtcc acgccagcac cggcttgctt aaaatttcgt
     2941 ttaaaataaa gccgctcatt ccggttgagc gattgaatga gctgccataa aaaggaggaa
     3001 ggtgcccgca tgttttattg ggttcaatga ttgcgtacaa ttgacttttg tgtacccaat
     3061 atatgaaata ttttaaataa gataaactgt taataatcat atgattataa aatttaaata
     3121 gacgaaatag caaaaaatga ttccgattat caggaaataa aaaaaacgcc cggctgatgc
     3181 agggcggtag atttgtagtg cattgattat ttaagccatc ctctttattt tatttatacc
     3241 cgtccaacca cgggggttcc aacagaggcc attacccatt aaccagctgt accactaatc
     3301 aggtacagct tttttttatt tactcaggta catactgcgg tctttataca actggcggaa
     3361 ataaggatca cgcaggtttt taataaagcg gatcgcttcc ccggttgatt tcatttccgg
     3421 accgagttct ttattcactc ccgggaattt attaaagctg aataccggtt ccttgatggc
     3481 aaatccatcc aggaattttt cttccggaat gtcctgcacc ctcatttcac ccagcatgac
     3541 tttagtggca tagttcaggt aaggcttgtt gtaagcttta gcaataaacg gagtggtccg
     3601 ggacgctctc ggattggctt cgatcacata tacttttcct tccttgatgg caaactggat
     3661 attgagcagt ccttttacat tcagcttcag ggcgatcttc ttggcataat ccaccatatc
     3721 ctgtacttcc agcggggtaa ggttgaagta aggcagtact gcattactgt cgccactgtg
     3781 aatacctgcc ggttcaatat gttccatgat tcccatcacg tggaattttt caccgtcaca
     3841 gatcgcatcg atctcggctt cctggcaacg atccaggaaa tgatctacca ggatcttgtt
     3901 tccgggaata tgtttcagca ggctgattac cgctttttcc acttcatcat cattaatcac
     3961 aatccgcatc cgttgtccac cgagtacata ggaaggacga accagtaccg ggtagcctac
     4021 tttattggct acttcaatgg cttcatccac atcccaggcc gttccataag cggggtaagg
     4081 gatctccagt tctttcagca tatcactgaa ccgtccacgg tcttccgcga tatccaggct
     4141 atcgtatgag gtgccgataa ttttgattcc tttctgtgtc agtttctcgg ccagcttcag
     4201 ggccgtttgt ccgcccagtt gaacgatcac gccgtacggc ttttcatgct ccacgatttc
     4261 ccagagatgt tcccaatata ccggttcgaa gtaaagctta tccgccatgt cgaagtcggt
     4321 gctcacggtc tcggggttac agttcaccat gatggcttca tatccacact ctttgatcgc
     4381 taacagtccg tgcacacagc agtaatcgaa ttcaataccc tggccgatcc ggttgggacc
     4441 actgccgagt acgatgattt tctttttatc cgacaccttg ctttcattgc tgtttccacc
     4501 ttcaaacgtg gaatagaaat agggtgtctt tgcttcaaat tccgccgcgc aggtgtccac
     4561 cattttgtac acccgggtaa tgccggcttc ctttctttta ttgtatacat cttcctcatc
     4621 cccgtgttca aggatacggc tgatctgttc atcactgaaa ccatttacct tggcttcccg
     4681 cagcagttca atgggtaaag tgtccaggtc gtatttggcg atttcttttt cgatggtaca
     4741 gatcttctgg atttcataga ggaaccagcg gtcaatacca ttggtggctt tggaaatagt
     4801 acccaccgag atacctgcca tcagggcatc cttgatccgg aagatccggt cccatttcgg
     4861 ggtcttgata tattccagca gttcttcagc atgcatcatg ctcttgccat aatagcctaa
     4921 acctaccgcg ttattttcga gactctgaca ggctttctga atggcttcgg taaagctccg
     4981 gccaatggcc attacttcac ccacactctt catctgcagt ccgagggtat cattggcgcc
     5041 tttgaattta tcaaagttcc agcgggggat ttttacaatt acataatcca gtgccggttc
     5101 aaaataagca gacgtggttt tggtgatctg gttttccagt tcatccaggt tgtatccgat
     5161 cgcgagtttg gccgcgattt tcgcaatggg ataaccggtg gccttactgg ccagggcaga
     5221 ggagcggctt acccggggat tgatctcaat tgcgatgatt tcttccgtat cgggactcat
     5281 cgcaaactgt acgttacaac caccggcgaa attaccgagg tcacgcatca tcatgatggc
     5341 ggtattccgc atcagctgca tgccggtatc actaagggtc atggccgggg ccacggtgat
     5401 ggagtcgccg gtatgcacgc ccatcgggtc aaagttttca accgtacaga tgatacatac
     5461 attatcggca gaatcgcgga gcagttccag ttcaaattct ttccagccaa gtacggcttt
     5521 ctccaccagt acttcatgga tgggcgaggc ctgtaaacca cggttcaggg cttcatcgag
     5581 atcttcttta tcatgtacaa atccgccacc tgtaccaccg agggtaaagg aaggccgaat
     5641 caccagcggg aagccgatct gctgggcaaa ctcttttcct tcgagataag agttggcggt
     5701 tttggccgta gctaccggta cgcccagctg aatcatccac tggcggaatt tttcccggtc
     5761 ttcggctttg tcaatggctt tgatatccac cccgatcagg cgcacattgt attgtttcca
     5821 caatcccagg tcttctgctt ctttggcaag gttgagggcg gtttgtccac ccatcgtagg
     5881 cagcaccgca tcaatctggt tttcctggag aatctgttca atgctctcca cggtgagggg
     5941 caaaaggtaa acccggtctg ccatcatggg gtctgtcata atggtggccg ggttactatt
     6001 aataagaatg actttaattc cttcttctct cagacttctg gcggcttgtg taccggaata
     6061 gtcaaattca caggcttgtc cgataataat ggggccggag ccgataataa gcaccgattt
     6121 gatcgaattg tctttaggca ttttttgttt ttaaattggt gagctggcag taatcgttgc
     6181 tgaaaaaaca aattgcgcaa aagtagggga atcgaagggg attgacggtt agttttcagg
     6241 aaaatttatc cccttgaaat tatcagggct caaaggaagc tggtttgggt attttccggt
     6301 aggaaagggt gaagagttca tcataaatat tccggttttg actcatatca agtaccagct
     6361 ttttaaaatc ttcggggcgg ggaaaggatt tgcgggtggt aaaggtttcg gtgaccgtcc
     6421 ggacctgcaa tgtatcctgg ctcatgctgt aagcagaaag ataccagacc ttactgggct
     6481 taccattcgg gccaagggcg gccaggttca taaaggattg atccctgacc cgggaactgt
     6541 aggcggtata aaaggaagtg tcgggcagga tcatatcacc cttgactgcc ttgccatagc
     6601 gaataactga ataaagggaa tccgattgcc ggctgatttc cagggcttct atgccgaaat
     6661 agtcgctgcc atctttcaca atgccatacc agaaaccaag gaggcttgag tcgaccggtt
     6721 ctaccggggc tttctccagg ggcatatttg actcaaaagg acaggccgtc aataaaaagg
     6781 acaggggcaa aagccacagt agtttggtca taacaggcga aaaagataca aaatgtccgg
     6841 taaaaggagg ggttaccttg atttttgctt tttcagctgg gtataaataa aaatggcggc
     6901 aaatccggcc agtataatgg agccggccag gatcaggttg tttcgacggt tccgggcttc
     6961 cagttccttg tttcgtcccg gatagcgatc ggtaatttcc gttttacctt ccgttgtatc
     7021 ttttactttt tccgggttcc ttacctgctc gttcgcttca tccttgatct ctgcctgtat
     7081 tttgtcgagc ttccggttat aataatgttc cttccaggaa gggaccggaa tcttgttctt
     7141 tttcagaaca ggtttcaatg cttccatttt tgattttacg ccaaaatgat cggacgaatc
     7201 attccgtaag gcaaaatcat aggcgatata ggccagttca accgattgct gggtggcata
     7261 gagatccccg agttcatcaa atacattagc cagcagtgca tccggaaaag gcgtaaaggg
     7321 aacccgctcc tgcaactggt attccacatc ccaggccata tcattgagtg tttccgcttc
     7381 ttttgaatca aactttaccc cggtacccaa tacacgattg ttataaatcc agtccggagc
     7441 agtggccatt ttgattttgg cttccaggat tttcacatgc acccattcag agccttcgtg
     7501 ggattcgggg ttgagttgta atcccttttt aataaattgt aaagccaatt catttttccc
     7561 cagtaattca taggtggtac cgagattggc ggcgatcgtg taatcttcag gataggcggc
     7621 ctgcagtttt tttaaaatat caagtgcttc ctggtaacgg ccgagataac cgaggtttac
     7681 ggcaatgtct gactgaatat ccttatcgta ggtttttaat gcagtcaggt tatattttgc
     7741 caggaaggat ttggcttccg ctttatccgg ctcattataa aaaagcgcca tgggttctac
     7801 ccggttggtt tgtttgccgc ttttgtttac atgtgattca ttgtagcagg caagcaccgg
     7861 ggtaaaataa attgcagtga agacgattac aattatcaat ttcatatggg atatggttta
     7921 ttttatacga ttcctcgtga ttttgggagt agatttgttt ttaaatttaa taaaaaagta
     7981 atcagcctga tggttaaagt gtttatcctg ttttttttcc tggtcattca atttgtagtt
     8041 tccgcacaat ccgggcccgt ctatccaaaa gggtatttcc ggaatcctgt tggtataccg
     8101 ttggatctct ccgccaattt tggggagttg cgctccaatc actggcatat gggactggat
     8161 atccgtacgc aggcgaaaga gaacctgcct gtgtatgcgg ctgcacaggg ttatattgcc
     8221 aaaatcggta tccgtcccca gagcttcggt cgctttatta tcatcaatca tccaaatggt
     8281 ctctcgactt tatacgggca tctcaataat ttttatcccg aactggaagc ctgggtcagg
     8341 gaacagcaat atgcgcagga gtcatgggcg gtagagctcg atattcccaa agagaaattc
     8401 ccggtcagta aaggcacctt tattgcttac agtggaaata ccggcggatc gcagggaccg
     8461 catgtgcatt ttgaaatcat cgataccaaa agcagtaagc ggctgaaccc gctcctgttc
     8521 ggatttccga ttgcggataa tgtacctcct acattgataa aactggcggt ttatgatcgc
     8581 agccgttcag tctacgatca gtcaccccgg ttctatccct taaaaaatac tgacagcggt
     8641 tatattattc cgaaactacc tgttatagaa acgggattat ccaggatcag ttttgccctg
     8701 caggcttatg accggc
//
LOCUS       DAOPMW010000109         8637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9032_length_8637_cov_8.333838, whole genome shotgun sequence.
ACCESSION   DAOPMW010000109 DAOPMW010000000
VERSION     DAOPMW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_8637_cov_8.333838"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLU11_06965"
     CDS             <1..1910
                     /locus_tag="PLU11_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58895.1"
                     /translation="ANGFSFTTTGRIANAFEATLEGQGSFSESAKGIYVRAALRNDLV
                     AQLNQLVTSKLNEAIQTTKNTLQQTRNTLKQAQNELKIIDDQIIARTNALKGQKAAEV
                     QAVRNEAIRAMNEQAAFIRTCEENNRSSQRKINELPDWRFLEKGAYYTAIAANNATIE
                     SAKVAYAGYLATVNATADVVENFPNDTELIRLYAEKTFKQSALAVANVGVDVAEGVSV
                     TTLEATKWVMDKSLGGLFDVKTVEFTGTFGLNNQYKVNAAINFTLTNDPYNMVAELDF
                     ADLASSAGKIVKDILGGTLIKPGFAPSFASNLKKPVTNTALKTAPAVLPPDNRNIAVV
                     QPTTTGSVINIPVTTTVADNWTGKIFRIENLWQAGQRINVEEGPLVASSITDGPWASQ
                     WIIKPVPGTQYYWIQNRLKGGRLNVEKGMLESSVIQDGAWSAHWELKKIGPDLYWIEN
                     RWQTGQRIHVESGKLSCSVISDGANSAMWYIRLSKPELSVPKNTADFSYNGLPLNPEV
                     FDSFVPGLESGEFSRTVNISTIKKITEAKQPWTTDFGFYSVYTPDGGSINYKYLGKTT
                     DGLYVFHVVRNWGGSFSFANVFIVERSGNVLNRKGMLSTPRFDGKRLEVQGNGIFYDG
                     TLYPVSSLFNN"
     gene            2188..3345
                     /locus_tag="PLU11_06970"
     CDS             2188..3345
                     /locus_tag="PLU11_06970"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPN58896.1"
                     /translation="MSHYYTLGSIPHKRHTQFRKPDGGLYSEQLFSTEGFSDDYALLY
                     HCHPPTMIIKTEAQVDVSPQIAEEKMLKHRSLEGFKVKPVKDYLQSRVPVLVNSDCHI
                     VLAAPQESMTDYFYKNTDADEMIFVHEGSGVVHTQYGQLPFSYGDYIILPRGTIYQIH
                     FNTPDNRLFIVESLSPLRYPKRYMSRYGQLMEHSPYCERDIRPPQDLQTIDEKGDFLI
                     KAKKKGMLYGLHYGTHPFDVIGWDGCCYPYIFSIHDFEPITGRVHMPPPIHQTFETNA
                     FVVCSFVPRLYDYHPDAIPAPYNHSNIDSDEVLYYVEGEFMSRKHVTKGMITLHPAGI
                     PHGPHPGAVEKSIGAKETKELAVMVDTFRPLMLTKQALEIENGDYVMSWSE"
     gene            3659..4348
                     /locus_tag="PLU11_06975"
     CDS             3659..4348
                     /locus_tag="PLU11_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58897.1"
                     /translation="MKNFIIVLLSALSLGARAQTGDNALLQEFLRSADYAHVLNHSAF
                     GKIGSPDLKQSYVGLATTGSGELQKQVPVIYLQFSGGQNGKKKILGQIQAIKVRDDYA
                     GLPRNSRYLMLFKDLREFNEVDGTGSIRVYDLNYDEYLAGEAVFNKGQLSQYTPYPVP
                     EPVSSRYNLGARSAAPCSNALTAGECFSCMMASCVFNPACRAICLATGSMSPNCTLSI
                     MALCAMASIVN"
     gene            4458..5276
                     /locus_tag="PLU11_06980"
     CDS             4458..5276
                     /locus_tag="PLU11_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58898.1"
                     /translation="MQLILTACLLLLPAMLWPQAKPPEPKGTDSSMAAKQVVPVANNY
                     KPEIFTSGFIDIVNNGQVNASARFIRLFIGEPGKFALPLSFYSGVSSNNFQQQVPGFQ
                     QSNESLVTNFINPLSGLANLSVDGVIYFTRKKEKLTKTGLLYHLGERVLTGVRTGLIT
                     DPLTGKPVNFLNGFGALGLYVQTGAWERSNNKNMGVTWLAWRYISCYTNPSQLKEFMP
                     DIQTNGVYKGWSLGWGVEITRLVNIKVLYYKYTKPPEIDYSVPIYQFSFNYSLK"
     gene            5457..6074
                     /locus_tag="PLU11_06985"
     CDS             5457..6074
                     /locus_tag="PLU11_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN58899.1"
                     /translation="MPKKQKDKTTEEQILAAAKKVFVRDGMAGARMQDIADEAGINKA
                     LVHYYFKNKEKLFEVIFMEAANRLFPRINEVFTAELPLFEKIEKFCEEYISLVMENPY
                     LPLFVLNEIHRDPNYFLGKVWKGKSTPKPEKFLAQIEAEKAKGTIKDIHPLHLLMNML
                     SMTIFPFVAKPMLQKNMGMNEQQFRMVMEQRKTEIPRFIIDSIKA"
     gene            6117..7379
                     /locus_tag="PLU11_06990"
     CDS             6117..7379
                     /locus_tag="PLU11_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN58900.1"
                     /translation="MKRLFLLLLIAFSLYAGAQPLQQLSLTTAWELAQKNYPAVKQKD
                     LVRQTAGISVENLQKGFLPQFSISGQATYQSDVTSVPISIPGFSIEAPNKDQYKLVAD
                     VNQLIYDGGLTREQKTLQQLNAKVEDQKVEVELYKVKERINQLFLSILYLDEQLKQVE
                     LVKKDIETGIKKVEAQVQNGVAFKSNLNMLKAELLKTGQRTVEISTSRKGLVAALSLF
                     VGQELNEQVRLEQPVSLLVADLNIDRPELKLYTEQENLIGQQDKLIQAKNQPKASLFA
                     QAGYGRPGLNMLKNEFAFYSIGGLRFNWSLGGLYTKKKEKEQVQVNKKIVEVQKETFL
                     LNTNAQLKQQQAEIDKLQQLISSDEAIIALRITVTEAAKAQLENGVITANDFLKEVNA
                     EDMARQSLITHRVQLLQSQINYQTILGK"
     gene            7408..8349
                     /locus_tag="PLU11_06995"
     CDS             7408..8349
                     /locus_tag="PLU11_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPN58901.1"
                     /translation="MKQIVFIALIAISVLTACKNGNGNADASGTFEANEVIVSAESSG
                     KLTSFTVEEGQLIAKDSVVGTVDATNISLQKEQVESSIQALGEKTVDVSPQVKLLEDQ
                     LAVQQSQLNNLLHEQGRIQNLLKQDAATGKQLDDINAQVDVAKRSMNVTRQQIQVQQN
                     NVATQNRSILSEGKPLQKRVAQLEDQLKKTTIMNPVAGTVLTKYAEQGEITSTGKALY
                     KIADLSTITLRAYITGTQLSQVKLGQSVKVLVDDGAKKYKEYSGTISWVSDKAEFTPK
                     TIQTKDERANLVYATKIKVKNDGYLKIGMYGEVKFAQ"
     gene            8408..>8637
                     /locus_tag="PLU11_07000"
     CDS             8408..>8637
                     /locus_tag="PLU11_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN58902.1"
                     /translation="MTAVSISNIIKRYGSKKAVTTALHGISFDVQQGELFGLIGPDGA
                     GKTSLFRILTTLLLAEEGTATVDGFDVVKDYK"
BASE COUNT         2469 a         1915 c         2134 g         2119 t
ORIGIN      
        1 aggccaatgg attttcattc accaccacgg gccgtatcgc caatgctttt gaagcgacgc
       61 ttgaaggaca gggaagtttt agtgaatccg cgaaagggat ttatgtacgg gcagctttac
      121 ggaatgacct ggtggcgcaa ttaaatcaat tggtaacctc aaaactgaat gaagccattc
      181 agactacaaa gaatacactg cagcaaacac gcaataccct gaaacaggca cagaatgagt
      241 tgaagattat tgatgatcag attatcgccc gtaccaatgc attaaaagga cagaaagcag
      301 ccgaagtgca ggccgttaga aatgaagcga tccgggccat gaatgaacag gctgccttta
      361 tccggacctg cgaagaaaat aaccgaagca gtcaacggaa aataaatgaa ttgcccgact
      421 ggcgtttcct ggaaaaaggg gcctattata ccgccattgc ggcgaataac gcgacgattg
      481 aatctgctaa agtggcctat gcgggttatc tcgctacggt aaatgcaacg gcggatgtgg
      541 tggaaaattt tccgaatgat acggaactga tccggctcta tgcggagaag acctttaaac
      601 aatctgcttt agccgtggcc aatgttgggg tggatgtggc cgagggtgtt tctgtaacca
      661 cactggaagc cactaagtgg gttatggata aatcactggg tggtttattt gatgtgaaaa
      721 cagtggagtt tacaggaacc tttggattaa acaatcagta taaagtaaat gcggctatca
      781 acttcaccct cacgaatgat ccgtataata tggtggcgga actggacttc gctgatttgg
      841 cttcaagtgc cggcaaaatc gtaaaagaca tattgggggg aaccctgatt aagcccggtt
      901 tcgctccatc atttgccagc aatctgaaaa agccggtaac gaataccgct ttaaaaacgg
      961 caccggccgt actgcctccg gataaccgga atattgctgt ggtgcagccc accacaaccg
     1021 ggagtgtgat caatatcccg gtaaccacta cggtggcgga taactggaca ggtaaaatat
     1081 tcaggattga aaacttatgg caggccggac agcggatcaa cgtagaagaa ggaccgctgg
     1141 tagccagtag tattacggat ggaccctggg cttcacaatg gatcatcaaa ccagtaccgg
     1201 gtacccaata ctattggatt cagaaccgct taaaaggcgg ccggttgaat gtagagaaag
     1261 gaatgctgga atcatcggtc attcaggatg gtgcctggag tgcgcattgg gaactgaaaa
     1321 aaatcggccc cgatttatac tggattgaaa accgctggca aaccgggcag cggatacatg
     1381 tggaaagcgg aaaactcagt tgctctgtga tcagcgatgg ggccaatagt gccatgtggt
     1441 atatccggtt gagtaagcct gaattgtctg tgcctaagaa cacagccgat ttttcgtata
     1501 acggattgcc cttaaatccg gaagtatttg attcttttgt tcctggtctt gaatcaggtg
     1561 agttcagtcg tacggtaaat atcagcacca ttaaaaaaat taccgaggct aagcagccct
     1621 ggaccactga ttttggtttt tattcagtgt atacccccga tggaggctct ataaattata
     1681 agtacctggg taaaacaaca gacgggcttt atgtgtttca tgtagtaaga aactggggag
     1741 gtagtttttc gtttgccaat gtatttatcg tggaaagaag cgggaatgtg ttaaacagga
     1801 aaggtatgct cagtacgccc cggttcgacg ggaaaagact ggaagtgcag ggtaatggca
     1861 ttttttatga cggaaccttg tacccggtga gcagcctttt taataattag ttgggtttgt
     1921 tgaccatata cgtgaagctt gtggtaaggt aaacgtttgt ttatttgtac aagtaaatag
     1981 tataaatttg tttatgccct ttcaccgcaa cttcacctat tatatcgaca agcgccagtg
     2041 gaaattctat ttcctcatgg tggctgttga aggactttgc ccccttgcct gaacgttttc
     2101 tttttgcttt tttcctcata gcactaagga acacataata aacaagaagt acttacttat
     2161 aacttatcac ttataactta ccactctatg tcccactact atactctcgg ctccatccct
     2221 cacaagcggc atacccagtt ccgcaaaccg gatggcggcc tgtacagcga acaactattt
     2281 tccaccgaag gattcagtga cgattatgcc ttgctgtatc actgtcatcc gccgaccatg
     2341 atcatcaaaa ccgaagccca ggtggacgta agtccgcaaa ttgccgagga aaaaatgctc
     2401 aaacaccgca gcctggaagg ctttaaagtt aaaccggtaa aggattacct gcagagccgg
     2461 gtgccggtgc tggtcaacag tgactgtcat attgtgttgg ctgccccgca ggagagcatg
     2521 acggactatt tttataagaa taccgatgcc gatgaaatga tctttgtgca cgagggaagc
     2581 ggggtcgtgc atacccagta cggtcaactg cctttttcgt acggcgatta tatcatactt
     2641 ccaaggggga caatctatca gatccatttt aatacaccgg ataaccggct atttattgtt
     2701 gaatcattgt cgccgctccg ttaccccaaa cgctatatga gtagatacgg gcaactgatg
     2761 gagcactcgc cctattgtga aagggatatc cggccaccgc aggacctgca gacgattgat
     2821 gaaaagggcg acttcctgat caaagccaag aagaaaggaa tgctctacgg attgcattac
     2881 ggaacacatc cctttgatgt aattgggtgg gatggttgtt gttatcctta tattttctcc
     2941 atacacgatt tcgaaccgat caccggaagg gtacatatgc cgcccccgat ccatcagacc
     3001 ttcgagacta atgcctttgt cgtatgttcc tttgtgccac gtttatacga ttatcatccg
     3061 gatgccattc ctgccccata taaccacagc aatatcgaca gcgatgaagt gttgtattat
     3121 gtggaaggag aattcatgag ccgcaaacat gttaccaagg gcatgatcac cctgcatccc
     3181 gccggtatac cccacggtcc tcacccgggt gctgttgaaa aaagcatcgg cgccaaagaa
     3241 accaaagaac tcgcggtgat ggtggatact ttccgtccgc tcatgcttac caaacaggca
     3301 ctggagattg agaatgggga ttatgtgatg agctggtccg agtaagttcg tagtcggtag
     3361 ttagtagttc gtagtcaaca gcccatgctg aggggtgttg gtagaatatt gtattatttg
     3421 aaaagcatca gtgaaaactg gtgcttttct tttagataac gtttcttagc aggcccttct
     3481 cgcttctagc ttctcgcttc tagctttccc tcagcctttc ttccgaaaaa aagcactcat
     3541 tcctgttgaa aaccacatgg tcattttttt tgtacggtct actttcggtt tgtcctggta
     3601 acaggaaccg tggatgccga aaaacgggag cagagtaggc gtttcaaaaa gtatagccat
     3661 gaaaaatttt attattgtat tgctcagtgc gctgagcctg ggcgcccgtg cgcaaacggg
     3721 tgacaatgcc cttttacagg aatttcttag atcggccgat tatgcccatg tactcaatca
     3781 ctcggcattt ggaaagatcg ggtcaccgga cctgaaacaa agctatgtcg gattggccac
     3841 taccggcagt ggggaattgc aaaaacaagt gccagtaatt tatctgcagt ttagcggagg
     3901 gcaaaatgga aagaaaaaaa tcctgggaca gatccaggcg attaaagtgc gggatgatta
     3961 tgccggtttg ccacgcaatt cccgttacct gatgttgttc aaagatcttc gtgagtttaa
     4021 tgaagtcgat ggcacgggaa gtatccgggt gtatgacctc aactatgatg aatacctggc
     4081 cggggaagcc gtattcaata aaggtcagct cagtcaatac acgccctacc cggtaccgga
     4141 gccggtatcg tcgaggtata acctgggagc ccgttcagcc gcaccctgca gtaatgcact
     4201 cactgccggg gaatgtttca gttgcatgat ggcgtcctgc gtttttaatc ctgcctgcag
     4261 ggcaatttgc ctcgccacag ggtccatgtc gccgaattgt accctgagta taatggccct
     4321 ttgtgcaatg gccagtatcg tcaattaatt tttagtagcc ggggagtcac cgacgacccg
     4381 gctgctattt ttcctcaccg tgccctgccg gaacgattgc cggtaagatc cggtatcatc
     4441 atgaatgaac cacccgtatg caactgatcc tgacggcctg tctcttgttg ctgccggcta
     4501 tgctatggcc gcaggcaaaa ccacctgagc caaaggggac agatagcagt atggctgcaa
     4561 agcaagtcgt gccagttgca aataattaca aaccggagat attcaccagt ggatttatag
     4621 atatcgtcaa taacggtcag gtcaatgcct cagcccgttt tatacgatta tttattggtg
     4681 agcccgggaa gtttgcgtta ccgctttcat tttattcagg ggtttcctcc aataatttcc
     4741 agcaacaagt ccccgggttc cagcaaagca atgaatcgct ggtgactaat tttatcaatc
     4801 ccctcagcgg actggccaat ctttcggtgg atggggttat ttattttacc cgcaagaagg
     4861 agaagctgac caagaccgga ttattatatc atctcgggga gcgggtattg actggtgtcc
     4921 gaaccggttt gatcacggat cccctgactg gtaagcccgt caattttctc aatgggttcg
     4981 gcgctcttgg cctctatgtc cagacaggtg cctgggaacg atccaacaat aaaaatatgg
     5041 gcgtcacctg gctggcctgg cggtatatca gttgttatac caatccttcg cagttaaaag
     5101 aatttatgcc ggacattcag acgaatggcg tctataaggg ctggtcttta ggttggggcg
     5161 tggagatcac ccggcttgta aatatcaaag tgctgtacta caaatacaca aagccgccgg
     5221 agattgatta ttcggtgccg atttatcagt tttcgtttaa ttactcacta aagtaggtgt
     5281 caggtgtctg gtgtcaggtg tctggagagt ctgaacccag gaagcgctat tactggcggg
     5341 aacaggatgc atcgaattat gtacgcccct gacatctggc agccgacaac tgacacctga
     5401 atttaaattt gaccatttgg ttaaaaattt ccatctttgt taaccatttg gttaaaatgc
     5461 ccaaaaaaca gaaagataaa accacagagg agcagatcct cgccgctgcg aagaaggtct
     5521 ttgtccggga tggtatggcc ggggcccgga tgcaggatat tgccgatgag gcggggatta
     5581 acaaggcact ggtgcattac tatttcaaga acaaggagaa gctgtttgaa gtgattttta
     5641 tggaagcagc caaccggctc tttcccagga tcaatgaagt atttacagcc gagcttccgc
     5701 tattcgaaaa aattgaaaag ttctgtgaag aatatatcag cctggtgatg gagaatcctt
     5761 acctgccgct ttttgtgctg aatgaaatac accgggatcc gaattatttt cttggaaaag
     5821 tctggaaagg gaagtccacc cccaaaccgg aaaaattcct ggcccagatc gaagcagaga
     5881 aagcgaaggg aacgattaaa gatattcatc cacttcacct gctgatgaat atgttatcta
     5941 tgaccatttt tccttttgtg gccaaaccca tgctccaaaa aaatatgggc atgaatgagc
     6001 agcaattcag gatggtgatg gagcaacgga aaaccgagat cccccggttc atcatagatt
     6061 ccattaaagc ctgatttttt ttgaaacact attaaccaac tggttaaaat atgaatatga
     6121 aacgactatt tcttctttta ctgatcgcct tcagcctgta tgccggcgct cagccactgc
     6181 agcaactgag cctgaccacg gcctgggaac tggcacagaa aaactacccg gcggtgaaac
     6241 aaaaagacct ggtgcgccaa acggccggca tcagtgttga gaatctgcaa aaaggattcc
     6301 tgccacaatt ctccataagc gggcaggcta cttatcaatc ggacgtgacc agtgtgccga
     6361 tttccattcc ggggttcagt atcgaagctc cgaataagga tcagtataaa ctggtggccg
     6421 atgtgaacca actgatctac gacggggggc ttacccgtga acaaaagacc cttcaacaat
     6481 tgaatgccaa agtggaagac cagaaagtgg aagtggaatt gtataaagtg aaagaaagga
     6541 tcaaccagtt attcctgagt attctttacc tggatgaaca actcaaacag gtggaactgg
     6601 taaagaagga tattgagacc ggcatcaaaa aagtggaggc gcaggtacaa aacggggtgg
     6661 cgtttaaatc caacctgaat atgctgaagg ccgagttgtt gaagacagga caacgcaccg
     6721 ttgagatcag cacgtcacgt aaaggactcg tggctgcact ttctcttttt gtgggacagg
     6781 aattaaatga gcaagtgcga ctggaacaac ccgtctcttt gctggtcgcg gatcttaaca
     6841 ttgaccggcc tgaactgaag ctgtataccg aacaggaaaa cctgattggt cagcaggata
     6901 aactgatcca ggcaaagaac cagcccaaag ccagtctttt tgcccaagct ggttatggac
     6961 gtccgggact gaatatgctg aagaatgaat ttgcttttta ctccatcggg ggactgcggt
     7021 tcaactggtc actgggtggc ctttatacaa agaagaaaga aaaagaacag gtgcaggtaa
     7081 ataagaaaat agtggaagta caaaaggaaa catttttact caacacgaat gcacaattaa
     7141 aacagcagca ggccgaaatc gataagttgc agcaattgat cagttcggat gaagcgatca
     7201 ttgcgctgcg tataactgta actgaagctg ctaaagccca attggagaac ggggtgatta
     7261 ccgccaatga ttttctgaaa gaagtgaatg cggaagatat ggcccgccag tccctgatca
     7321 cccacagggt gcagttattg caatcacaga tcaattatca aaccatttta ggtaagtaat
     7381 tccatcccga aatttaaata ctgaagtatg aagcaaattg tatttatcgc ccttatcgcc
     7441 atcagtgtac tgacggcctg taaaaacggc aatggaaacg cggatgccag cggcacgttt
     7501 gaagccaatg aagtgatcgt ttccgcagaa tcatccggta aactgacttc tttcaccgtg
     7561 gaagaggggc agctgatcgc caaagacagc gtggtgggca cggtggatgc caccaatatc
     7621 agtctgcaga aagaacaggt ggaatccagc atccaggcac tgggagagaa aacggtggat
     7681 gtgagtccgc aggtaaaact ccttgaagac cagctggctg ttcagcagtc tcaactgaat
     7741 aacctgttgc atgaacaggg acgtatccag aacctgctga aacaggatgc ggccaccggt
     7801 aagcaactgg atgatatcaa tgcgcaggtt gatgtggcta aacggagtat gaatgtaacc
     7861 cgccagcaga tacaggtaca gcaaaacaat gtagccacgc agaaccgcag tatcctgagc
     7921 gaagggaaac ctttacaaaa aagagtggcc caactggaag accagttaaa gaaaaccacg
     7981 atcatgaacc ctgtcgcggg aacggtactg actaaatatg ccgagcaggg agaaatcact
     8041 tcgactggta aagcattgta taaaattgcg gatctgtcca ccattacatt acgcgcctat
     8101 attaccggca cacaattgtc gcaggtaaaa ctgggccagt ctgttaaagt gcttgttgat
     8161 gatggcgcta aaaaatacaa ggaatattcc ggcaccatca gttgggtctc cgataaagct
     8221 gagtttacac cgaagaccat tcagaccaaa gacgaacgcg ccaatcttgt atatgctacg
     8281 aagatcaaag tgaagaatga cgggtacctg aagatcggta tgtacggcga agtgaagttt
     8341 gcccaatgaa ataacaaaaa gcccagtccc aacacctaac agctaacacc caacagctaa
     8401 acctgatatg accgcagtta gtatatcaaa tatcatcaaa cgctacggct ccaaaaaagc
     8461 cgtcactacc gctctgcacg ggatcagttt cgatgtgcag caaggggagt tgtttggact
     8521 gatcggtccg gatggcgccg gtaaaacctc gctgttcagg atactgacta ctttgttact
     8581 cgcagaggaa ggtacggcta cggtggatgg ctttgatgtg gtgaaggact ataaaga
//
LOCUS       DAOPMW010000110         8627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9051_length_8627_cov_7.584928, whole genome shotgun sequence.
ACCESSION   DAOPMW010000110 DAOPMW010000000
VERSION     DAOPMW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9051_length_8627_cov_7.584928"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1097
                     /locus_tag="PLU11_07005"
     CDS             48..1097
                     /locus_tag="PLU11_07005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN58903.1"
                     /translation="MSYKFLSICMKVISVSLFLLFLQPVSAQYNWGDLDAQLEAKQKI
                     LGNEFVVMLWKGDTLVYKKEKGTFNSKTQAPIASCSKWLTAALVMQFVDEGKISLDDK
                     ISKYIPELNKYFKSYISFRDCLSHMTGIEDEGGFLKKMMQRKKYNTLEEEVNSYAARD
                     IRANTGTDFWYGSIGLNMAGRALEVMTKRKFDVLIRTKLLIPLGMRKTTFTNLEGGAV
                     NPSGGAQSTADDYMKFLVMLLNKGKYNGMQILSENAVNEMMKIRTSAAMIKYAPKAAT
                     GFNYASGAWVVEENNGKATALASPGLFGTWPMIDYCRGYAYLVFVKNILGEERADAYL
                     ELKKTVDNQIQENCR"
     gene            1149..1991
                     /locus_tag="PLU11_07010"
     CDS             1149..1991
                     /locus_tag="PLU11_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58904.1"
                     /translation="MQFFKPGLLAFTLSCTTFIMAQPITLSVKSGQKYKVENTTKMNT
                     SAEAMGQTMETIMNSNNTTVYEIKNVSAAALELSATITTIVVNGSTMGQEMSFDSEKK
                     DNSGPMADVLTPILNKSKSYTMDNKGTIVKQDETEEAEGQAAMMGISNVNYTNTDLFL
                     PALVGKSLKPGDSFDDTGSLKKEKYSTRDSGTYKVTSIENGIASIVYTGIQTIIAIGE
                     QMGMEVTTNSNNIVKSEYQVDVNTGIVLSKATVIESSVSVDIAGMTIPATGKTIVTVK
                     VTAF"
     gene            2164..2508
                     /locus_tag="PLU11_07015"
     CDS             2164..2508
                     /locus_tag="PLU11_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58905.1"
                     /translation="MYEHRKQPLASKATYYQRVLKNILFAGLILSLCLLIGIAGYHYW
                     GEASWIDATHNAAMILSGMGPVIEITTVSGKIFSSVYALFSGVVFITNVGIILSPALH
                     RLYHRLHLEDEN"
     gene            complement(2523..4541)
                     /locus_tag="PLU11_07020"
     CDS             complement(2523..4541)
                     /locus_tag="PLU11_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HPN58906.1"
                     /translation="MKIRLCLLLCAAMFELSAQDAADESRFPTVTPVMLGALYYPIDS
                     GATAVVLAEYSETKVNGSNDGGFEFETRIRRRIHLLKKASFDRGTVVVDLFTNEEEQE
                     KVSKLNAVTWVLEGGKVTETKLNTKSDVYTEKLNRNHIRKKFTLPGLKEGAIIEYEYT
                     LTSGYLFNLRPWYFQGEVPCLWSEYRVAFPEFLNYIMLQQGILPFTIADTELKPGTYN
                     LTFMRDVYAGKTVDERLMINCRIAHHRWVISNVPAFKEEAYTSSPDNYMNRIWFQLSG
                     FQQPLVEKTLQATWPEFTASVLNRLDFVNEMENTGEWWPEEMKQVLKGATSESDIAKA
                     IYSYVSTRFSLNSDESVRRNPLKKVAAAKSGNMLELNLLLVAMLRYAGLQADPVMLST
                     REHGYSTEAFPITGQFNYLVCRVKADEDDWLLDATHPYLGFGKLPYQCYNGQARVLNR
                     EATLIHLLPDQLTESDEMSVDINFNMADSPQWKSTVNHRYGFFASEELREKIKKAGLE
                     SFRKELALDEIAGESVSDLTVESLEKFGTSLQVTYKTETGVKADNELYLKPVLVPYFR
                     QNPLKSAERKYPVEMPYKISQQYTVHIRVPEGYEVEELPASFSADINGKKDAAFDYTV
                     TTANGKVTIQYGLDITKTYFKPDEYRGLREFFSKIAAKLEEQIVFKKK"
     gene            complement(4565..6493)
                     /locus_tag="PLU11_07025"
     CDS             complement(4565..6493)
                     /locus_tag="PLU11_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPN58907.1"
                     /translation="MRKWVSLYLFSGLISLTALAGDGDYAVFKIPPALLKNANAVVRV
                     HEQYVELQKEDRLFIREHYVITILNEEGNHFSEFFEPYHKFLEIKSVEGALYDGMGKK
                     LKSLKSKDVEDGSGSGGESLADDNRYKKHNFYYRVYPYTIEYTIESVKKETMFFPSWV
                     PVFGELISVEKSSFAVDVPSGYLLRYKEFNYSREPDIKESGKSKIYTWSLANFESIKR
                     EYAAPSWKNIAPYVLFAPSSFVIEDYKGNMNDWSSFGKFILSLNKGRDQLPEPVKAKV
                     AELTRGAGTNEEKVIRLYRYLQANTRYISIQLGIGGWRPLEASFVANKLYGDCKALSN
                     YMYALLKEAGIPSFYTVIKSGDGADDIQVDFSSSQFNHVILSVPLPGDTLWLECTSQT
                     QSPGYMGDFTGNRHAVIITEEGGKLVTTPRYGMKENTQVRNVKAVLNEEATLEIEARS
                     TYSCMQQDDLHMMINALSKDKVKEYLQHQLDFATYEVGRFNYKENQSRFPTIDEELDI
                     TVSNYATITGKRLFIVPNIMSRHARRLTPNESRKYEVELDMEYRDIDSVEITIPAGYS
                     VESVPQEVVLDTKFGRFRSSVKMEGNRILYYRSLDHYAGKFPAKEYAELVKFYDAMYK
                     ADRAKLVLVKNETTPKSF"
     gene            complement(6498..8513)
                     /locus_tag="PLU11_07030"
     CDS             complement(6498..8513)
                     /locus_tag="PLU11_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HPN58908.1"
                     /translation="MSSKGIFLCLIAWSGSLLLSAQDKPPVKFGNVTAKDFATTVYPI
                     DSNAQAVVIADIGYTFMEGNNKSSISVITKRYRRVHILNKNAFDAASESIFFYKNMDV
                     EEELDKLKAVTYNLENGKVVETKLDTKNGVFKETINRNRFVKKFTMPNVKEGSIIEYE
                     YTVRSDFFTQLDPWLFQGPYPVLWSEYNLELPEFLGFTFLSRGYKKYDITKKNTRSTT
                     FTIIDSRGTGASERGQFSTNVTDHRWVIKNVPALKEENFTSTLRNHITRIEFQLAETR
                     PPYAYKNFVQSWSKVTTDLLLDESFGEKIARDNGWLDEYTNPLVLGAKSDLEKARRIY
                     YYVRDNFTCTNYSDFYADQSLKNIIKTKKGTVSEINLLLTAMLKHEKILADPVMLSTR
                     SHGQTYELYPLKSQYNYVVVKADIGNTAYFMDATEPRLGFGHLPVRCYNGHARVVNKT
                     ADPVYFTPDTLMERSSTSVFMINDEKGNMVGSLNRVPGYFESFSLREKVNEKGKESLE
                     TEIKKSFTQEVSIAKLKLDSLNKPEELVWVNYDFDILDPKEDIIYFNPLLASEGYKEN
                     PFKSALRFYPVEMPYGFDRTYNLQMEVPEGYVVDELPKSMVVKMNEAEEGMFEYRISQ
                     SGTSISFRCRVQLKRAYFDPEEYEMLREFFNLIVTKESEQIVFKKKK"
BASE COUNT         2344 a         2054 c         1827 g         2402 t
ORIGIN      
        1 taaacttcat agtcaaatac taatccaagg ccttaaattc agtaacaatg tcatataaat
       61 tcctttctat ctgcatgaaa gtcatatctg tatccctttt cctgttattc ttgcagccag
      121 tcagtgccca gtacaattgg ggcgatctgg atgctcaact ggaagccaaa cagaaaatac
      181 tgggaaatga attcgtagtg atgctctgga aaggagatac attggtttac aaaaaagaaa
      241 aaggaacgtt caattccaag acacaggcgc ctattgccag ttgcagcaaa tggctgacag
      301 cggcactggt aatgcagttt gtggatgagg gaaagatcag cctcgatgat aagatttcga
      361 aatatatccc agagctgaat aaatacttta aaagctatat cagcttccgg gactgcctga
      421 gtcatatgac tggcatagag gacgaaggag gtttcctgaa aaaaatgatg caacggaaaa
      481 aatacaacac cctggaagaa gaagtaaatt cctatgcggc ccgtgatatc agggcaaata
      541 ccggcactga tttctggtat ggcagtatcg gactgaatat ggcgggaagg gcgctggaag
      601 tgatgacaaa acgaaaattt gatgtgctga tccggaccaa actactgatt ccgctgggta
      661 tgcgtaaaac aactttcacc aacctggaag gcggtgctgt taatccttcc gggggtgccc
      721 aatccacggc ggatgactat atgaagttcc tggtgatgct gctgaataaa ggcaaataca
      781 acggcatgca gatcctgagt gaaaatgccg tgaatgagat gatgaagatc cgtacttctg
      841 cggccatgat taaatacgca ccgaaagctg ctaccggatt caattatgca tccggtgcct
      901 gggtagtgga ggaaaataac ggcaaagcca ctgcgctggc cagcccgggc ttatttggca
      961 cctggcccat gatagattac tgcaggggat atgcctacct ggtttttgtg aagaatatac
     1021 tgggtgaaga gcgggcagat gcctatttgg agctgaaaaa aacagtggac aatcaaatcc
     1081 aggaaaattg ccggtaattt cagtacacta tacggaaaca gtaatatatt tattttaaaa
     1141 aaaccgatat gcaatttttt aaaccaggtt tattggcgtt cacattgagt tgtaccacct
     1201 tcattatggc tcaacctata accctgagtg ttaaaagcgg acaaaaatac aaagtggaaa
     1261 acacaaccaa aatgaacacc tccgcagaag caatgggtca gacaatggaa accattatga
     1321 acagtaacaa tacaacagtt tatgagatca aaaatgttag tgctgcggct cttgaactgt
     1381 cagccaccat tacgaccata gtggtaaatg gcagtactat gggacaggaa atgagtttcg
     1441 acagtgaaaa aaaggataac agcgggccta tggcggatgt actgacaccc attttaaata
     1501 aatcgaaatc ctataccatg gacaacaaag gcacgattgt aaaacaggat gaaacggaag
     1561 aagccgaagg ccaggccgcc atgatgggta taagcaatgt caactatacc aacacagatc
     1621 ttttcctgcc cgcactggtg ggtaaaagcc taaaaccagg agacagtttt gatgatacag
     1681 gatcattaaa aaaggagaaa tacagtaccc gcgattccgg cacctataag gtcacttcca
     1741 ttgaaaatgg aattgccagc attgtctata ccggtataca gacgattatt gccatcgggg
     1801 aacaaatggg aatggaagtg actacgaaca gcaacaacat cgtgaaatca gaataccagg
     1861 tagatgtaaa tacaggtatt gtactttcca aggctaccgt gattgaatcc agcgtgagtg
     1921 tggatattgc aggtatgacc atccccgcaa caggcaaaac aattgttacc gtaaaggtca
     1981 cagcttttta atcattaaat tacagattat tcctctaagg ctttccgcat ggagggcctt
     2041 tttatttaaa gtcttttttg ctggcaattt ttactaccct gcaagctcac ggatgctcag
     2101 ggaataacct ggcaatgata tctctgctta ttaagttatc tttagtgaac taatcggaat
     2161 ggcatgtatg agcaccgcaa acaacccctt gcttccaaag ccacctatta ccaacgggta
     2221 ctgaaaaata tactgtttgc cgggcttata ctcagcctct gcctgctgat cggtattgcc
     2281 ggctaccatt actggggaga agccagctgg attgatgcca cccacaatgc cgctatgatt
     2341 ctgagtggta tgggtccggt cattgagata acaacggtta gcggaaaaat attctcatcg
     2401 gtttatgcct tattcagcgg ggtggtcttc atcaccaatg tcggcatcat attgtcccct
     2461 gccttgcacc ggttgtatca ccggttgcat ctggaagatg aaaattgaac gcataaatta
     2521 aatcatttct tcttgaaaac gatctgttct tctagtttgg cggcaatttt actaaagaat
     2581 tcacggagac ctctgtattc atccggcttg aaataggttt tagtaatatc aaggccatat
     2641 tgaatcgtga ccttcccatt ggctgtggtt acggtatagt caaatgcagc atcctttttc
     2701 ccgtttatat cagccgaaaa cgaagctggt aattcttcta cctcgtatcc ctcaggcacc
     2761 cgtatatgaa ccgtatattg ctggctgatc ttatatggca tttcaacagg gtactttcgc
     2821 tcggctgatt ttaatggatt ctggcgaaaa tagggtacca gcacaggttt caggtagagc
     2881 tcattatcgg ctttgacccc cgtttcggtc ttgtaagtca cttgcagtga tgtaccaaac
     2941 ttctccaatg actcaacggt aagatctgat acgctttcac cggcaatctc atccaatgcc
     3001 agctctttcc tgaacgattc aagccctgcc ttttttattt tctccctcag ctcttcagaa
     3061 gcaaagaaac cataccggtg attgaccgtt gacttccatt gaggactatc agccatgttg
     3121 aaattaatgt caacagacat ttcatcggat tcagtcagct gatcaggaag caggtggatc
     3181 agcgtcgctt cccggttcaa tacccgggcc tgtccattat aacattgata tggtagtttt
     3241 ccaaaaccca ggtaaggatg tgtggcatcc agtagccagt catcctcatc tgcctttacc
     3301 cggcaaacca agtagttgaa ttggccagtt attggaaatg cctcggtgga ataaccatgt
     3361 tcacgggtac tgagcatcac cgggtcggcc tgcaaaccgg cgtagcggag catggctacg
     3421 aggagcaggt tcaactccag catattaccg ctttttgcag ccgccacttt tttcaatggg
     3481 tttcttctga cactctcatc actattgagt gaaaaccgtg tactaacata gctatatata
     3541 gcttttgcga tgtctgattc ggaagttgcc cctttcagca cctgtttcat ttcttctggc
     3601 caccattccc cggtattctc catttcattg acaaaatcaa ggcggttgag tacagatgcg
     3661 gtaaactcgg gccaggtagc ctgtaatgtt ttttctacca gtggttgctg gaagccggaa
     3721 agctgaaacc agatccggtt catataatta tccggagaac tggtatacgc ttcttcctta
     3781 aaagctggaa cattactgat cacccaccgg tgatgcgcga tcctgcagtt gatcatgagc
     3841 cgttcatcca ccgttttacc cgcatataca tcccgcatga aagtcaggtt gtaagtaccc
     3901 ggtttcagct cagtatcggc aatggtgaac ggcaaaatgc cttgttgcag catgatataa
     3961 ttcaggaatt ccgggaatgc cacccggtat tctgaccaca ggcagggcac ttccccctga
     4021 aaataccagg gccggagatt aaacaggtag ccggaagtga gcgtgtattc gtattcaatg
     4081 atggcgcctt ctttaagacc gggcagggtg aattttttcc gtatatggtt ccggttcagt
     4141 ttttcagtat aaacatccga cttcgtgttc agttttgttt cggtcacctt acctccttcc
     4201 agtacccatg tcaccgcatt cagtttgctt actttttcct gctcttcctc gttggtaaac
     4261 agatcaacga cgacggtacc ccggtcaaat gaagccttct tcaataaatg gatcctccgg
     4321 cggatcctgg tctcgaactc gaaaccacca tcattgctgc cattgacttt tgtttcgcta
     4381 tactcggcca gcaccacggc tgtcgccccg gaatctatgg gatagtacag ggcaccaagc
     4441 atgacgggcg tcactgtggg gaaacggctt tcatctgcag cgtcctgcgc tgataattca
     4501 aacatcgctg cgcatagcag caggcataaa cgaattttca taaaacacct tataaagaca
     4561 aggtttaaaa acttttcggc gttgtctcat tcttcaccag gaccagcttc gcacgatctg
     4621 ctttatacat cgcatcatag aattttacca gttccgcgta ttcttttgcc ggaaacttac
     4681 ccgcataatg atccagacta cggtagtaga ggatgcggtt gccttccatc tttactgaac
     4741 tacggaaccg tccaaacttt gtatccagca ccacttcctg aggtaccgat tctacgctat
     4801 agcctgccgg tatagtaatc tccacgctat ctatatcccg gtattccata tccagttcca
     4861 cttcatactt ccggctctca tttggcgtga gccggcgggc atgacgggac ataatattcg
     4921 gtacaataaa caaccgcttg ccggtaatgg tggcataatt actgaccgta atatccagtt
     4981 cttcatcaat ggtcgggaac ctggattggt tttccttata attgaaccgg cccacttcat
     5041 acgtggcaaa atccagctga tgctgcaggt attctttcac tttgtctttt gacaaggcat
     5101 taatcatcat gtgcaggtca tcctgttgca tacagctgta ggtcgatctg gcttctattt
     5161 ccagggtggc ttcttcattc aaaaccgctt tcacattccg cacctgggta ttctctttca
     5221 ttccgtaacg gggggtggtc accagttttc cgccttcctc ggttattata acggcatgcc
     5281 ggttacccgt gaagtcgccc atataacccg gcgactgggt ctggctggta cattccagcc
     5341 acaaagtatc ccctggcagc gggaccgata aaatcacatg attaaactga ctggaagaaa
     5401 aatccacctg aatatcatcg gctccgtccc cggatttgat taccgtatag aatgatggaa
     5461 tgcctgcctc ctttaacaat gcatacatgt aattggataa cgctttgcaa tccccatata
     5521 atttattggc cacaaaggag gcttccagtg gccgccaacc tccaatcccc agctgaatac
     5581 tgatatacct agtgtttgcc tgcaggtaac ggtaaagccg gattactttc tcctcgttcg
     5641 tgccggcgcc ccgggtcaat tcggccactt ttgcttttac cggttccggc aactgatcac
     5701 gtcctttatt gagtgataaa ataaattttc caaatgatga ccagtcattc atattgcctt
     5761 tataatcctc gatcacaaag gaggaaggag caaacagtac atatggagcg atatttttcc
     5821 acgacggtgc ggcatattcc cttttaatag attcgaaatt ggccaggctc caggtatata
     5881 ttttactctt gccgctctcc tttatgtccg gttcacggga atagttgaac tccttatacc
     5941 tgagcagata accggaaggg acatctactg caaaactgct cttctccact gagatcagtt
     6001 cgccgaaaac cggtacccag gaggggaaaa acatggtttc cttcttcacc gattcgatgg
     6061 tatactctat cgtatacgga tataccctgt aatagaaatt gtgtttctta taccggttat
     6121 catccgccag gctctccccg cctgaaccgg agccatcttc aacgtcttta ctcttcaggc
     6181 tttttaattt tttccccatc ccgtcgtata aagccccttc cacggatttt atttccagga
     6241 acttatgata gggctcaaaa aattcggaaa agtggttacc ctcctcattg agaatcgtaa
     6301 taacataatg ttcccggata aacaagcggt cttctttctg caattccaca tattgctcat
     6361 gtaccctgac caccgcattc gcgttcttca ataaagccgg gggtatttta aataccgcgt
     6421 aatccccatc gccggccaat gctgtcagac taatcagtcc gctgaacaaa tagaggctaa
     6481 cccattttct catttgctta ctttttcttt ttgaatacaa tctgttctga ctccttcgtc
     6541 acaatcaaat tgaaaaactc ccgcaacatt tcatattcct ccggatcaaa ataggcccgt
     6601 ttcagctgca cccggcaacg gaatgaaatg ctggtgcctg actgggaaat gcggtattca
     6661 aacattccct cttccgcttc attcattttg accaccattg attttggcag ttcatccacc
     6721 acatagcctt ccggcacttc catctgcagg ttataggtcc ggtcaaatcc ataaggcatt
     6781 tccaccggat agaaccgcag cgcggattta aacggattct ctttataccc ttcactggcc
     6841 agcaggggat taaaataaat gatgtcttct ttcgggtcaa gaatatcaaa atcatagttt
     6901 acccacacca gttcctctgg tttgttgagg gaatcgagtt tcaacttggc aatggagact
     6961 tcctgggtaa aggatttttt tatctccgtc tcgagtgact ctttcccttt ctcattcact
     7021 ttttcccgca aactgaaaga ctcgaaataa cccggcaccc tgttcaggct gcccaccata
     7081 ttccctttct cgtcgttgat catgaataca ctggtactgc ttctttccat caatgtatcg
     7141 ggtgtaaaat aaacagggtc tgctgtcttg ttcaccaccc gggcatggcc gttgtaacaa
     7201 cgtaccggaa gatgaccaaa tcccaggcgg ggttccgtag catccataaa ataggcggta
     7261 ttcccgatat ctgctttcac caccacataa ttgtattggc tcttcagcgg atagagttca
     7321 taggtttgcc cgtgcgaacg ggtactgagc ataaccggat cagccagaat tttttcatgt
     7381 ttcagcatgg cggtcagcaa cagattgatc tcggaaacag tccccttttt ggttttaata
     7441 atattcttca ggctttgatc cgcataaaag tcactgtaat tggtacaggt gaaattatcc
     7501 ctgacataat aatagatccg gcgggctttc tccagatctg atttggcgcc cagcacaagc
     7561 ggattagtat attcatccaa ccaaccgtta tcccgggcaa ttttttcccc gaacgactca
     7621 tcgagcagca agtcggtggt cactttagac caggactgta cgaaattctt atacgcataa
     7681 ggcggacgtg tttcagccag ttgaaactcg atccgggtga tatgattccg caaagtggaa
     7741 gtaaaatttt cctcttttaa ggccggcaca tttttgatca cccaacgatg atcggtcaca
     7801 ttggtggaaa attgtcctct ttcagaggcg ccagtacccc tggaatctat aatggtaaaa
     7861 gttgtactac gggtattttt cttcgttata tcgtattttt tatacccacg tgataaaaaa
     7921 gtaaacccca ggaactccgg cagctccagg ttatactcac tccataaaac cggatagggg
     7981 ccctgaaata accagggatc cagttgcgtg aaaaagtccg accggaccgt atactcgtat
     8041 tcaatgatgg acccttcttt aacattcggc atcgtaaact tctttacaaa gcgattcctg
     8101 ttgattgttt ctttgaacac gccattttta gtatccagtt tagtttccac cactttccca
     8161 ttctccaggt tataggtgac cgctttcagt ttgtccaact cttcttccac atccatgttc
     8221 ttgtagaaaa aaatggactc actggctgca tcaaaggcat ttttattgag gatatgcact
     8281 ctccggtatc gtttggtgat aaccgagatg ctgctcttgt tatttccttc cataaatgta
     8341 tagccgatat ccgctatgac taccgcttgt gcattactgt cgatcggata aacagtagta
     8401 gcaaaatctt tggccgtaac atttccaaat tttacgggtg gcttgtcctg tgcggataaa
     8461 agcaacgacc ctgaccaggc gatcaggcag agaaagatcc cttttgaact catagtgatg
     8521 gttttgtagt taaattagcc gaataaaggc caattttaca gaaaattata aaaaaagagg
     8581 ctaaacaaaa atcaggtcgc caactaattt aaaacagcct gttaaat
//
LOCUS       DAOPMW010000111         8567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9143_length_8567_cov_8.971687, whole genome shotgun sequence.
ACCESSION   DAOPMW010000111 DAOPMW010000000
VERSION     DAOPMW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9143_length_8567_cov_8.971687"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7128)
                     /locus_tag="PLU11_07035"
     CDS             complement(<1..7128)
                     /locus_tag="PLU11_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPN58909.1"
                     /translation="MTKREITLTNVDYGTYSVNLTFGFMIEGVVAFDANNVLAYGNYG
                     YVPAILKSSNGGLSYQLVYHNPIGPNIFSSLFNLVFPQNNQTGYAVDGYNILKTTNGG
                     TTWTRVYTGSTFSIGQLDAIDNNTVFAFSDDESATASTGKLIKTTNAGNSWTQLNTPD
                     NRIYAADFLTPEKGWLNVYLNADSLLIYYTSTGGVTWTKKNMAAVNPIGFHRMHFVDD
                     STGYGVANLFYTFKTSDSGRIWEPLPRDNSFNYLNYGHTEIHFANSSQFWSAGAREFI
                     ELTTNGGGVPLPRAYFTIDTSGLYQTGTVKLQNYSKASYTHKWYRNNELISTSYHANF
                     QRVPSSTHDSIKLVVSNGVLSDSLTLHQHFWPPVSVTSFTPTIGASGAVINISGQNFT
                     NVVSVFFGNVPASTFTVNSAQSISATVAGGASGFVKVLTANSRDSLAGFTFIPAPTIS
                     SFLPVSATAGTTITILGTNLTGATSVIIGGVSAVFSVNSATQITATAPSGPSGSISVT
                     TPGGSATLSGYSSLPGINTFSPTSGTIGTILSITGSSLTGTTAVTIGGVAVSSFTVNN
                     ANSITAIVSTGATGPVAITRPEGSASLPGFTWIPAPVITSFTPQSDPVGTNVTITGTG
                     FNNNLTGNTVYFGGARANIVSASSTQITVTVPYSAGYSTISVTANNLSGYSTNMYLTT
                     FSGGGSITQSSFGTAETIQTGFEVLPFSTGAADFDLDGKSELIVLKLNNPGNTHGVLI
                     YKNTGTGSAITMGTPIELAGGNESFATGDVNGDGKTDIILSGAPYLYTYINTSSAGNL
                     SFAPVIMTSSTLTLQRIRLADMDVDGKPDVICSSNGNIYVIRNISEPTQVNYAEPVMI
                     FTASFERNFIPADLTGDGKPELIMSGFGVLRNNSSPGTLNFTLAATLPLYNHSFISTG
                     DIDGDGKLDVVSGDRYGSQINVIRNTSSGSNISFATPVSYQASSLPAGSAIGDLDGDG
                     KPDIAVSTAENAVTLFKNISVPGSVNLLSPKSFVPGTYSGENQVALADFNNDGKSDIA
                     ISSENPRTVFVLRNEVKADPSIISFTPTIGENGTAVTITGTNFTGATAVRFGAVPASS
                     FVVNSPTSITAVVGSGASGFVTVVTGIGIDSLPGFVYGIPPVITSVSPSSGHVGTMVT
                     INGSNFSTNPSDNIIYFGRVKAQALTATSTTITTYVPPGSDYAPVSVTKARLTGWSNK
                     PFLTTFPNNGSALTTLSFANRINIGNFPVGCLVDFDGDGKLDVAGVLNTSTVAIARNI
                     SVPGQIRFAPAVVLSGTIGGGGIMAEDFDGDGKKDLLFIQNNIQLMLVKNNSTPGNIV
                     VAGLYSIPSGNSGVIGIISARDIDKDGKPDILFASYSGAVNIIKNNCSGETINFSPHY
                     YYPLGNYGLSAISTDVDDDGNPDLLAASGELAVFRNTSTPGNIIISPRVGFSGGDWPT
                     YISTGDLDGDGRTDALVSQSEPNKMAVFKNLSAPGAIVFSVHPELPSGNHPYTNGMND
                     LSGDGKPDIFAPNLSEDSLSIFTNNSTPGSLVFAPHIKIASGNVASRMESADLDGDGR
                     PDIIISGNSGPATIIRNQHSVTGPQINNFSPVSATASATITITGERFTDISNVTFGGV
                     PASSFNVISTTRIEAIVGNGASGPVSVHSTAGISSLTGFIYGNKPVLSSFMPETGFEG
                     STITINGANLTGTTAVNFGNTPAASFVVNASGTSISAVVGNGNSGQVKLVTPAGSDSL
                     GNFIYLTKPTISSFSPLLGNAGSTVVITGTNFNNVSAVLFGGASAISFTVVSETSISA
                     IVGNGHTGQIEVITAGGSAFANGFIYNNSASISDFNPKSAGTGSAVYLYGFNLASVTS
                     VKFGGVPASSFTIESPTRIKALVGTGASGEISVTNHLYTTSKPGFTFLNGTVPSITAI
                     SPVSGSTGSVITISGKLFTGVSEVRFGNSIAEAFEVLSDSMIIATVGSGSTGLVTLLS
                     PGGAVSADGFTYSPGPVINSFYPVSAPPLTPVTIVGQNFSNTISGNTVFFGTVKATVT
                     EASPNYLIVKVPYGAKMSPITVTSGQLTAYSSVEFIPTFNGSSIISPDAYIRSIDSTS
                     PKTPSLITLGDLDLDSKPDIVVSSPSGSTLTSRLSFHLNVSSSSNIWLAPRTSIHTPI
                     GPYGSTYADFNNDGKPEIAASGGASFSQFILFPNNSSPGNLSWGTTLTFPMSPESEKC
                     FSADFDKDGKTDIGFLGGWNGAISIYRNTGNATTFSFSAAQVFSTFLYASSVAITDLD
                     GDGKPDIVVAGGNSTMPTFHVLKNTSTNGSITFQTIVLNLPFNVNQVLTGDLDNDNKP
                     EVLLINFYDNKLLVLKNQSIPGFVLLETVLFLPVKNPGSIALADISGDGKPDLCVADT
                     YNNYVSVLANRS"
     gene            complement(7404..>8567)
                     /gene="mnmG"
                     /locus_tag="PLU11_07040"
     CDS             complement(7404..>8567)
                     /gene="mnmG"
                     /locus_tag="PLU11_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPN58910.1"
                     /translation="TKQRSCWITYTNQKVHDILKTGFDRSPMFAGRIDGVGPRYCPSI
                     EDKINRFAERDRHQLFVEPEGWNTVEIYLNGFSTSLPEETQYEALRSIPGFENARMFR
                     PGYAIEYDFFPPTQLKYSLETKLINNLFFAGQINGTTGYEEAACQGLMAGINAHQKAV
                     EKEALILKRSDAYIGVLIDDLISKGTQEPYRMFTSRAEFRTLLRQDNADIRLTEISYK
                     LGLASQERMDRVTDKKSSVEQIKKILKEMSVEPAEINGWFEQIGSSLITEKQKAEKIL
                     LRPSVELADMATILPKLKTALYSFTPDSIEQAAIQVKYDVYIEKEKELVSRMSQLEEL
                     EIPENFDYKKIGSLGNEAREKLSRVRPRTLGQASRISGINPSDVQILMVFMGR"
BASE COUNT         2325 a         1842 c         1917 g         2483 t
ORIGIN      
        1 tgatctgttg gccaaaacac tcacatagtt attataggtg tcggccacac agagatcagg
       61 ttttccgtct ccactgatat ctgcaagggc aatactgccc ggatttttta cagggagaaa
      121 cagcacggtt tctaacaaca caaatccagg tatcgattga ttcttgagca ccagcagttt
      181 gttatcatag aagtttatca ataagacctc tggtttatta tcattatcca gatctcctgt
      241 tagtacctga tttacattaa aaggcagatt cagtacaata gtttggaagg tgattgatcc
      301 gtttgtactg gtgtttttaa gtacatgaaa ggtgggcatg gtcgaattgc caccggcaac
      361 tacaatatca ggtttgccat ctccatccag atctgtaata gcaacactac ttgcatataa
      421 gaaggtagag aagacctggg cagcactgaa actgaacgtt gtcgcatttc cggtgttccg
      481 ataaatgctt atagcaccat tccaacctcc gaggaatcca atatctgttt ttccgtcttt
      541 gtcaaaatcg gcagaaaaac atttttcgct ttcgggagac attggaaatg tcaaggttgt
      601 accccaactg agatttcccg gactgctgtt gttcggaaac agaataaact gactgaatga
      661 tgcccctccc gatgccgcta tttccggttt cccatcatta ttgaagtcag cataagtgga
      721 tccgtatggc ccgatgggcg tgtgtatgga tgttcggggt gccagccaga tattcgagga
      781 actggatacg ttaagatgaa aactcagccg cgatgtcagc gttgaaccac tggggctact
      841 gactacgata tctggtttgc tgtccaggtc cagatcccct aatgttatca aagagggtgt
      901 tttgggagaa gtcgaatcaa tgcttcggat atacgcatcc gggctaatga tggaggatcc
      961 gttaaacgtt ggaatgaatt caaccgagga ataagcggtt aactgtccgg aagtaactgt
     1021 tataggagac atctttgccc catacggaac ctttacaatc agataatttg gacttgcttc
     1081 tgttaccgtt gctttaactg taccaaaaaa gacggtattc ccggaaatgg tattactgaa
     1141 attctgtcct acaattgtta caggcgttag aggtggggca ctaaccggat aaaatgagtt
     1201 aatgacgggc ccgggagaat aggtgaatcc atcagcagaa acggcgccac cgggggacag
     1261 aagtgtaact aacccggtgc tgccagatcc aactgttgcg atgatcatcg aatcggatag
     1321 tacctcaaat gcttcggcga ttgaatttcc aaagcggact tctgatacac ccgtaaagag
     1381 ttttcccgag atcgttatta cggagccagt agaaccagat acgggtgata tagcggtaat
     1441 tgaaggaacc gttccattaa ggaaggtgaa gccgggttta ctggttgtat ataagtgatt
     1501 ggttacactg atttcgccag aagcacctgt tccaacaagt gcttttatcc gggttggtga
     1561 ttctatggta aaactgctgg cgggtacacc tccgaatttt acactggtta cacttgcaag
     1621 attgaagcca tagagataaa cagcgcttcc tgtgccagct gattttggat taaaatctga
     1681 aatacttgca ctgttattat aaatgaaccc atttgcaaaa gcggagccac cagcggtgat
     1741 cacctctatt tgtcctgtat gaccatttcc tacaatagcc gatatggatg tttcagatac
     1801 tactgtaaaa ctaatggcag acgccccacc aaacagtacc gctgaaacat tattaaaatt
     1861 agtaccggta ataactacgg tagagccggc gttgcccaat aatggactaa aagaactgat
     1921 tgttggttta gtcagataaa tgaaattacc cagggagtct gagcctgccg gggtcactaa
     1981 ttttacttgc ccggaatttc cattcccaac tacggctgag atacttgtgc cggatgcgtt
     2041 taccacaaaa gaggccgcag gtgtattacc aaaattaacg gcggttgtgc ccgttaagtt
     2101 tgctccgtta attgttatcg tcgatccttc aaatccggtt tcaggcataa aggaactgag
     2161 gacgggtttg ttgccataaa tgaatccggt cagactgctg attcccgcag tactgtgtac
     2221 agatactgga cctgacgccc cgttaccaac aatggcctca atccgggtag tagatattac
     2281 attaaacgaa gaggcaggaa cgccgccaaa tgttacatta ctgatatctg taaaacgctc
     2341 cccggttatt gtaatagtgg cactggcggt tgcactaaca ggagaaaagt tgtttatctg
     2401 gggacctgtt acactgtgtt ggttcctgat tatcgtagcc ggtcctgaat ttcccgaaat
     2461 gatgatatcg ggccttccgt caccatcaag atctgcagac tccatacggc tggcaacatt
     2521 tcccgatgct attttaatgt gtggcgcaaa taccagtgat cccggcgtgc tgttattggt
     2581 aaaaatggat aatgaatcct ctgacagatt aggtgcaaaa atatctggtt tgccgtcccc
     2641 ggaaagatcg ttcattccgt ttgtataggg atgattccct gatggcaact cgggatgaac
     2701 agaaaaaacg attgcccccg gagcgcttaa atttttaaat acggccatct tgttgggctc
     2761 agattgagag acgagtgcat ccgttctgcc gtctccatcc agatcaccgg tagatatata
     2821 agtgggccaa tcgcctccac tgaaaccgac ccttggactt attatgatat ttccgggggt
     2881 actcgtattc cgaaatacgg ccagctcacc tgaagctgcc agcagatcag gattgccatc
     2941 atcgtctaca tcggtagaaa tcgcggacaa accatagttg cccagaggat aataatagtg
     3001 cggagaaaag tttatagttt cacccgaaca gttattttta ataatattga ctgcaccaga
     3061 ataactcgcg aacagaatat ctggttttcc gtctttgtca atgtccctgg ccgaaattat
     3121 tcctataaca ccagagttgc cggatggaat actgtataag cctgcaacca caatatttcc
     3181 cggggtactg ttattcttta ccaacatcag ttggatattg ttttgtataa ataacaggtc
     3241 ttttttacca tcaccatcaa aatcttcggc cattattccc ccaccgccaa ttgttccact
     3301 taatacaaca gctggtgcaa aacggatctg accgggaaca ctaatgtttc tggcaatagc
     3361 aacggttgat gtatttagca caccagccac atccagtttt ccatcaccat caaaatccac
     3421 cagacatccc accgggaaat tgccaatatt gattcggttt gcaaatgaca gtgtggttag
     3481 cgcggaccca ttattgggga aagttgttaa aaacggctta ttggaccagc cagtcaggcg
     3541 tgcttttgtt actgataccg gggcataatc tgaccccgga ggtacatagg tagtaatcgt
     3601 tgttgatgtg gctgttaaag cctgtgcttt tacacggcca aagtagataa tattatcaga
     3661 aggattggtg ctgaagttag aaccattgat tgtaaccatt gtgcccacat gaccagatga
     3721 tggcgaaacc gatgtgatta caggtggtat tccatataca aaaccgggta atgaatctat
     3781 tcctattcca gttactactg tcacaaaacc acttgcgcca ctacctacta ctgcagttat
     3841 agaagttgga gagtttacta caaaactgct tgccggtacc gctccgaatc ttacagcggt
     3901 tgccccggta aaattggtac cggtaatcgt tacggctgtt ccgttttcac caatagttgg
     3961 ggtaaaagaa ataatggacg gatctgcttt cacttcgttt cgtaaaacga ataccgttct
     4021 tggattttca gatgaaatag cgatatctga tttcccatca ttattgaaat ctgcaagggc
     4081 aacctgattt tcgccactat atgttccggg tacaaaactt tttggtgata ataaatttac
     4141 agaacctgga actgaaatat ttttaaacag ggttaccgca ttttcagcag ttgatacggc
     4201 aatatccggc ttaccatcac cgtccagatc gccaatggcg gaacctgccg gtaaggaact
     4261 tgcctgataa gacacaggcg ttgcgaaaga aatgttgctg ccggaactgg tattgcggat
     4321 cacattaatt tgtgagccat aacggtctcc tgaaacaaca tccagtttac catctccatc
     4381 aatatcaccg gtggagatga atgaatggtt ataaagaggt aatgtggccg ctagtgtaaa
     4441 gttcagcgta cccggtgacg aattattacg taacacgcca aatccagaca tgatcagttc
     4501 tggcttacca tccccggtga ggtcagcagg tataaaattt cgttcaaatg aagcggtgaa
     4561 tatcataaca ggttccgcat agttgacctg tgttggttca ctgatatttc tgattacata
     4621 aatattgcca tttgagctgc aaatgacatc cggttttcca tctacatcca tatcagctaa
     4681 tcgtatacgc tgaagtgtca gggtagaaga tgtcatgatt actggtgcaa atgagaggtt
     4741 gcctgcagag gatgtgttta tataggtata cagatatgga gctccggaca gtataatatc
     4801 tgttttgccg tctccattaa catccccggt tgcgaatgat tcattgcctc ctgctaattc
     4861 aatcggcgtg cccatggtga ttgcagatcc tgttccagtg tttttatata ttaatactcc
     4921 atgtgtgttg cccggattat tcagtttcag aacgatgagt tcacttttgc cgtcgaggtc
     4981 aaaatcagct gctcccgtac tgaatggcaa cacttcaaaa ccggtttgaa tggtttctgc
     5041 ggtgccgaat gaagactgtg tgatactacc cccaccactg aaagtggtta aatacatgtt
     5101 agttgagtat ccggatagat tgttagctgt aacactgatt gtgctgtacc cggcactata
     5161 tggaacagta acggttattt gagttgacga tgcggaaaca atattagccc ttgcgccgcc
     5221 aaaataaaca gtatttccag ttagattatt attaaaaccc gttcctgtta tcgtcacgtt
     5281 cgttccaacc ggatcggatt gtggggtaaa tgaagttata actggtgccg gtatccaggt
     5341 aaagccgggc aaagaagcgg agccctctgg cctggttata gcaactggcc ctgttgcccc
     5401 cgtacttact atagcagtaa tactattggc attatttacg gtgaatgaag atactgctac
     5461 acctcctatc gtaacggcgg ttgtacccgt aagtgagctt ccggttatgg acaagatggt
     5521 tccaatggta ccactggttg gagaaaaggt attgattccg ggtaaggaag aatagccgct
     5581 caacgttgca gaaccgccgg gtgttgtcac gcttatgcta cccgatggac ccgaaggagc
     5641 cgttgccgtt atttgtgtag ctgaatttac agaaaaaacc gcagaaacac ctccgattat
     5701 tacagatgta gcgccagtca gatttgttcc cagaatggtg atcgttgttc cggctgtagc
     5761 ggatactggt aagaacgagg aaatagtagg tgctggaata aatgtaaagc cagccaaaga
     5821 atccctgctg tttgctgtta aaacctttac aaagccactg gccccacctg caactgttgc
     5881 ggatatactt tgggccgaat tcacggtaaa agtagatgcc ggtacatttc caaagaaaac
     5941 ggatactaca tttgtaaaat tctgaccgga tatgtttatt actgcaccac ttgctcctat
     6001 tgtaggggta aaggaggtaa cacttaccgg aggccagaaa tgctgatgaa gggtgaggga
     6061 atcagataat acgccgtttg aaacgactaa tttgatacta tcatgcgtgc tggaaggtac
     6121 tcgttggaaa tttgcatgat aagaggtact gattaattca ttatttctgt accatttatg
     6181 ggtatatgaa gcttttgaat aattctgcaa ttttaccgtt ccggtttgat ataaaccgct
     6241 tgtatcaatg gtaaagtacg ccctgggtaa cggcacacca cccccatttg tggttaattc
     6301 tataaattcc cgtgctcctg cgctccagaa ttgtgagcta ttggcaaaat ggatttctgt
     6361 gtgaccataa ttaaggtaat tgaaactatt gtctcttggg agaggttccc aaatacggcc
     6421 cgaatcactg gttttaaagg tatagaataa attcgctacc ccatatcctg tactatcatc
     6481 aacgaagtgc attctgtgga acccgattgg atttactgct gccatatttt tctttgtcca
     6541 ggtcacaccg ccggttgatg tataatatat taacagacta tcggcattca gatatacatt
     6601 caaccatccc ttctctggtg ttaaaaaatc agctgcatag attctgttgt ctggggtatt
     6661 gagttgagtc cagctgtttc cggcattcgt ggtttttatc aattttcctg tagatgcagt
     6721 ggcactttca tcatccgaaa aggcaaatac agtattgtta tctattgcat ccagttgccc
     6781 gattgaaaag gtggaccctg tatataccct ggtccatgtt gttccgccat tggttgtttt
     6841 tagaatatta taaccatcta ccgcatatcc cgtctggttg ttttgcggga aaaccagatt
     6901 aaataatgag ctgaatatat ttgggccgat cgggttatgg taaacaagtt ggtacgaaag
     6961 tcccccgttg gatgatttca atatcgcagg tacatagcca taattaccgt atgcgagtac
     7021 attattggcg tcaaaggcaa caactccttc gatcataaag ccgaatgtga gattgacaga
     7081 ataggtgcca taatctacat tggttagcgt gatttctctt tttgtcaaag ttcttccgct
     7141 atctgttgta tagcctatcc aatcatgagg atttccggtg cttgcaatat atccggttga
     7201 cggggtgtaa aagctgatcc ttttaatatt gtttccttca attgaaaggt tggaatgaat
     7261 ttgacgaagc tgggcatttg cctgaataaa caataaggac aagataagtg caatcaggaa
     7321 tttttgatgc attttgttgc agataaggag tatagaaaac gaaaaattaa taaaattaat
     7381 tgacttcgtt tatgaccatt ttcttaccta cccataaaaa ccataagtat ctggacatca
     7441 cttggattta tcccagaaat ccggctggcc tgtcccaagg tccgtggccg aacccttgat
     7501 agtttttccc gggcctcatt tcctaaggag ccaatctttt tataatcaaa gttttccgga
     7561 atttccagtt cttccagctg ggacattctg ctgaccagtt ctttttcctt ttctatatac
     7621 acatcgtatt tgacctggat ggcagcttgt tcaatggagt ctggtgtgaa agagtacaag
     7681 gctgttttaa gttttggaag gatagttgcc atatcggcca gttccactga cgggcgcaat
     7741 agaatttttt ccgctttttg tttttcagta atcaacgaag atccgatttg ttcaaaccag
     7801 ccatttattt ctgccggttc caccgacatt tcttttagta tttttttgat ctgctcaaca
     7861 gaggattttt tgtcagtcac ccggtccatc ctttcctggc tggctagtcc gagtttgtag
     7921 cttatttctg tcaatctaat atctgcattg tcctgacgaa gcaaggttct gaattctgcc
     7981 cgggaggtaa acatgcgata cggctcctgg gttcctttgc tgatcaggtc gtcgatgagc
     8041 acgccaatat aagcgtcact tctttttaat atcagggcct ctttttccac ggctttctga
     8101 tgggcgttga taccagccat taatccctga caagccgctt cttcatatcc ggtggtgcca
     8161 ttgatttgtc cggcaaaaaa cagattgttg atcagcttcg tctccaatga atacttcaat
     8221 tgagttggcg gaaagaaatc atattcgatc gcataccccg gacggaacat acgggcgttc
     8281 tcgaacccgg gtatactacg aagtgcttcg tattgtgttt cttctggtaa ggaagtagag
     8341 aagccattaa gataaatctc tacagtgttc cagccttccg gttctacgaa gagctggtga
     8401 cgatctcttt cggcaaagcg gttgatctta tcttcaatgc tgggacaata gcgcggtccc
     8461 accccatcga tccgaccagc aaacatcgga ctccggtcga agccggtttt taaaatatcg
     8521 tgaaccttct gattggtata ggtgatccag caacttcttt gtttagt
//
LOCUS       DAOPMW010000112         8449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9317_length_8449_cov_7.025018, whole genome shotgun sequence.
ACCESSION   DAOPMW010000112 DAOPMW010000000
VERSION     DAOPMW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9317_length_8449_cov_7.025018"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PLU11_07045"
     CDS             complement(<1..1328)
                     /locus_tag="PLU11_07045"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN58911.1"
                     /translation="MIIPFQDDSKRYYLFTTDYQWGTNGLCYSVINMTLNNGKGDVES
                     KNTQLTPYVREKITSVNHCNGKDTWVITRDYRNGDFMAFLVTAAGIQTPVITASLSTA
                     SVLGYLKASPDGTRIADAKWYSGLELFDFNSTTGLVNNNKTIMTYSNLFSYGVEFSPD
                     SKKLYTTCYRDNFPLSSVFYNYILQFSNLDSSVPVIQSTLTWLDSAIANNYGVFNALQ
                     LAPDGKIYVSIFNSTKLSVINNPDQVGSACQYSRSGPLLMTGSSSRFGLPDFNQSYVK
                     GRFRYDTACFSNQVRFYYSRANDVHSVKWDFGDPASGAGNTSALDSPQHVFSAPGVYS
                     VKLVTYLPCRNDTFIKKVTIDPFTVDLGPDKAVCADSLFLLDPRAGAGKQYKWQDNST
                     LSTLSSTLPGLYWVEVSRTDGSCKTRDSILISSKPNPTVYLGRDSLLCEN"
     gene            1736..2920
                     /locus_tag="PLU11_07050"
     CDS             1736..2920
                     /locus_tag="PLU11_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPN58912.1"
                     /translation="MAARTDLFQSPDYFAVDELLSEEHLMIRDAVRSYVKKEISPIIE
                     DYAQKAEFPQHIVKQLGELGCFGPTVPVQYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMFPIYAYGSEEQKMKYLPKLGSGEWLGCFGLTEPDHGSDPSSMLTNIKDAG
                     DHVILNGAKMWISNAPFAQVAVVWARNEADEIVGVIVERGMEGFSTPTTHGKWSLRAS
                     ATGELVFDNVKVPKENILPGVKGLKGPLSCLTKARYGIAWGVIGAAMDCYDTALRYSQ
                     ERIQFGKPIGAFQLQQKKLAEMITEITKAQLLNWRLGVLMNEGKVTPQQVSMAKRNSC
                     EIATNICRDARQMLGGMGITGEYPVMRHMMNLESVITYEGTHDIHLLITGMDVTGYNA
                     FK"
     gene            2968..3474
                     /locus_tag="PLU11_07055"
     CDS             2968..3474
                     /locus_tag="PLU11_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPN58913.1"
                     /translation="MKVYLIGFMGSGKTHWGRLLSEKLGVRFFDLDEEVVEEAGKPIT
                     EIFATAGEEHFRMLEKEVLHHLTEKHESFIMACGGGTPCYFNNIEFMNQAGTSVWINT
                     PVETLFSRLVNQKSKRPLIRDLSDDQLRGFISKKFSDRRMYYEQAELVVDEEPVELDK
                     LIETIFHA"
     gene            3467..3859
                     /locus_tag="PLU11_07060"
     CDS             3467..3859
                     /locus_tag="PLU11_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HPN58914.1"
                     /translation="MHKNYLGIAALLGAITVAIGAFGAHGLQKITSDAVILHSYQTAV
                     QYQMVHVLALLGVGILFERFSASWLIWAANLFLVGIFLFSGSIYIITYLKIKGMSTGW
                     IGPVTPIGGLLFIAGWLALLMAVQRKQH"
     gene            complement(3856..5697)
                     /locus_tag="PLU11_07065"
     CDS             complement(3856..5697)
                     /locus_tag="PLU11_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPN58915.1"
                     /translation="MTAQDSSHVYLDISKVILEERFNKDISSFYTTQKGAINELYPRM
                     VFEPGIQHSGTLPNDWVTKRIFLRFNVCNNTDSNSAVYFCPGYYFTKINLYRVEGNQL
                     IPLPDIRPAINERDGFRLIELGPNDSALLVTELSGVKTYNNLLRPRVIGKDHVTAFVA
                     QLHGKHNQQNLITYVFCGLLLMMILFSLTNFFQGGNKEFLFYSGYALFMGGLLFLQTV
                     FHLYASPFGFFIEGYLDFLLQATGIIFYMLFMKKFLNTDQTHPFLSKLYNTGISILVG
                     SMLLYTVLHYFTGNYVLEYLVEILSKVLLLTLILVYLVYSIRNWKEKLLRYLFWGNLF
                     LFIFSTISQLGIVYDAQFRKLPGLLNASIIYYELGLFLELVLFLAGLNYKNRRNIINE
                     TKEKETLKAQNLLKEYEKEIAVYKAQQEERQRISADMHDELGAGMTAIRLMSEIARNK
                     MKENTPIEIEKISRSADDVLNKMNAIIWSMNSGNDTLDNLVSYIRSYSLEYFDGTNVE
                     CRLKAPDQIPDKELAGDKRRNIFLCLKETLNNTLKHAEASLVEIDISIGNELCIRIAD
                     NGKGIDLDKLRQFGNGLKNIARRMESIGGSYHIENKKGTVTTLILPL"
     gene            5946..>8449
                     /locus_tag="PLU11_07070"
     CDS             5946..>8449
                     /locus_tag="PLU11_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPN58916.1"
                     /translation="MANKLRKKSKDRAEPTPPVSPDLKEFRKEKEEAVDWKAIARDER
                     TWKIVGAVFLLISIFLFISFTSYFFTWKEDQAVAQQGFSALLDTDEPVANLLGRLGAV
                     TSHFFIFKAFGIASFLICTFFFVVGVNLLFRRKVFSIWRNLKYVTLGLLILSVSLSFV
                     FQHTEFAFGGAVGNLINQKLYGAVGTIGTAAILLLLATGYFIWQFNPAFNLPQKKQPQ
                     PGAETATVNITEEATPVLATQTVNDLYAGKDKAKPNSLKGESPVVINFPDEEPLHDLK
                     MVEKELPLEVVQPPVQQQESIPEIEVVKDLMHTHDLDITLPEPEEEIIQKPRDLTPTK
                     KGDQELELEIKAVPEKVEEEEIAGVENLPPYEPTLDLRDYKYPTLNLLENHGGEKIVQ
                     DTSELENHKNQIIATLRNYSIEIQKISATIGPTVTLYEIVPAPGVRISRIKNLEDDIA
                     LSLAALGIRIIAPIPGKGTIGIEVPNIRKTMVSMRSLLASEKFQNNAFNLPIAIGKKI
                     DNENFIVDLTTMPHLLMAGATGQGKSVGVNAILVSLLYKKHPSQLKLVLIDPKKVELS
                     IYRRIEKHFLAKLPGEEEAIITDTKKVVNTLNALCIEMDNRYDLLKEAGSRNIKEYNE
                     KFIKRKLNPQKGHQYLPFIVLVIDEFADLIMTAGKEIEMPIARLAQLARAVGIHLIIA
                     TQRPSVNIITGTIKANFPARIGFKVSSKIDSRTILDTGGAEQLIGKGDMLISYNGELT
                     RLQCAFVDTPEVDEVVDFISNQRGYSQAFLLPEYVDEKDLEGKDFDMGDRDSLFEDAA
                     RLIVQNQIGSTSLLQRRMKLGYNRAGRLMDQLEAAG"
BASE COUNT         2288 a         1836 c         2098 g         2227 t
ORIGIN      
        1 ttgttttcgc agagcaggct gtcgcgtccc aggtatactg ttggatttgg tttggaactg
       61 atcaggatac tgtctcttgt tttacagctg ccatccgttc tggaaacttc cacccaatac
      121 aaaccaggga gtgtactgct caaggtggat agggtggagt tgtcctgcca tttatattgt
      181 ttgccggctc cggcacgtgg atccagcagg aataaggaat ctgcacatac tgctttatcc
      241 ggcccaaggt ccacagtaaa tggatcaatt gtaactttct tgatgaaagt gtcattccgg
      301 caaggaagat aagttaccag ctttactgaa tatacaccgg gtgcggaaaa gacatgttgc
      361 gggctgtcga gggcggaggt attgccggca ccgcttgccg ggtcaccgaa atcccatttc
      421 accgaatgta catcattggc cctggaataa taaaatctga cctggtttga aaaacaggcc
      481 gtatcataac ggaaacggcc tttcacataa ctctgattaa agtcgggtaa accaaatcgt
      541 gaagaagaac ctgtcattaa cagcggtccg gatcttgaat attgacaggc agagccaacc
      601 tgatcggggt tgtttatgac tgacagtttt gttgaattaa agattgatac ataaattttt
      661 ccatctggtg ccagttgcag ggcattgaat acaccataat tgttggcaat ggcgctgtcc
      721 aaccaggtca gtgtggattg aatgaccggt acagaggaat caaggttgga gaactgtaga
      781 atataattgt aaaaaactga agataacggg aagttgtccc tgtaacaggt ggtatacaat
      841 ttttttgaat cgggtgaaaa ctctacacca taactaaata aattggagta ggtcataatt
      901 gttttattat tattgaccag accggtcgtc gagttaaaat caaatagctc caggcctgaa
      961 taccatttgg catctgcgat tctggttcca tcaggggaag ctttcaggta ccccagtaca
     1021 gaagccgtac tcagcgatgc agtgattacc ggggtttgta ttccggcagc agtgacgagg
     1081 aaagccataa aatcgccatt cctgtagtcg cgggtaatta cccaggtgtc cttcccgtta
     1141 caatgattta ctgatgtaat tttctcccta acgtaaggag tgagttgggt gtttttggat
     1201 tccacatcac ctttaccatt attcagcgtc atatttatta cggagtaaca tagtccattg
     1261 gtaccccact gatagtctgt ggtgaaaaga taatatcgtt ttgaatcgtc ttggaaaggg
     1321 atgatcatac ctgattgtgt acttgaagca tgcccgaaaa gcccggtacc gttcggcatt
     1381 agtgtatgat ctgcacacca tacagacatt ccatcagaat aaaaaagaag ttgaccgttt
     1441 acgtcactga tggacgtact cccttcagat tgattcatgc tgcttagctg tgtactggct
     1501 ggctgacccg tggtaaagct cagaactgct tttaggccaa aaacccagtt gtcagcccgt
     1561 tgggcagata gaatacctga tagtaaaagc aggcagaaaa gtaaacattt tttttgcatg
     1621 gggataaggt atttaaaggt acagattcgt attgttttat acaaacagtc gttttgtaca
     1681 gtacgctgta ttctactaac tttgccttgg cttttttaac taaaaaaaat aaacgatggc
     1741 agcccgcacc gacttattcc aaagccctga ttatttcgca gttgatgaat tactgagcga
     1801 ggaacatctt atgatccgcg atgcggtccg ttcctatgtg aagaaagaaa tttctcccat
     1861 tattgaagac tatgcccaga aagcggagtt tccgcagcat attgtaaaac agctgggcga
     1921 actgggttgt tttggcccaa ctgttcccgt gcaatacggc ggcggcggac tggattatat
     1981 ttcctacgga ctgatgatgc aggaactgga aaggggagac agcggcgtgc gttctaccgc
     2041 ttctgtacaa ggctccctgg tcatgttccc gatctatgca tatggcagcg aagagcagaa
     2101 aatgaaatac ctgcccaaac tgggaagcgg cgaatggctg ggttgttttg gcctgaccga
     2161 gcctgaccat ggttccgatc ccagcagtat gctgacgaat atcaaggatg ccggtgatca
     2221 tgtaatcctg aatggcgcta aaatgtggat cagcaatgcc ccctttgctc aggtggcggt
     2281 ggtttgggcc cgcaatgaag cggatgaaat tgtcggggtg attgtggaaa gagggatgga
     2341 aggattttcc accccaacca ctcacggtaa atggagtttg cgggcttcgg ctaccgggga
     2401 actggtgttt gataatgtaa aagtgcccaa ggaaaatata ttgcccgggg tgaaaggatt
     2461 gaaaggcccg ttgagctgtc tgaccaaggc ccgttacggc atcgcctggg gggtgattgg
     2521 tgcggcgatg gattgttatg ataccgcgct tcgttattcc caggagcgta tccagtttgg
     2581 caaacctatc ggggctttcc agctgcagca gaagaagctg gcggaaatga tcaccgagat
     2641 cacaaaggct caactgctca actggcgctt aggggtgctg atgaatgaag ggaaagtaac
     2701 tccgcagcaa gtgagtatgg ccaaacggaa ttcctgcgag atcgcgacta atatttgtcg
     2761 cgatgcccgc cagatgctgg gaggtatggg cattaccggc gaatacccgg tgatgcggca
     2821 tatgatgaac ctggaaagtg tgatcacgta tgagggcaca catgatattc atctgctgat
     2881 cacggggatg gatgtgacgg ggtataatgc gtttaagtga gggatgcgag aagcaagaag
     2941 ctagaggcga gaaggaaata caaagaaatg aaagtttatc taatcggctt tatgggttcg
     3001 gggaaaacgc actggggtcg tttactcagt gaaaaactgg gggtccggtt ttttgacctg
     3061 gatgaagaag ttgtggagga agccggtaaa ccgattactg aaatttttgc cacggccggg
     3121 gaagaacatt tccggatgct ggaaaaagaa gtgttgcatc acctgaccga aaaacacgaa
     3181 agttttatca tggcctgtgg cgggggcact ccctgttatt ttaataatat tgaatttatg
     3241 aatcaggcag gaacttctgt ctggatcaat acgccggtag aaactctttt ttcccggttg
     3301 gtcaatcaga aatcaaaacg acccctgatc cgtgatttgt cggacgacca gttaagggga
     3361 tttatcagta aaaaattctc agaccgccgg atgtattatg agcaggctga actcgtagtg
     3421 gatgaagagc cggtggaact ggataaactc atagaaacta tctttcatgc ataagaatta
     3481 tctcggtatt gcggctctcc tgggggccat cacggtcgcc atcggggcat ttggcgcgca
     3541 tgggttacag aaaattacat cggatgcggt tatcctgcac agttatcaga ctgccgtaca
     3601 gtaccaaatg gtgcatgtgc tggctttatt aggagtaggg atactattcg aacggttttc
     3661 tgcatcctgg ctcatttggg cggctaatct gttcctggtt gggatatttc ttttttccgg
     3721 atccatctat atcattacct atctcaaaat aaaaggaatg agtacgggat ggatcggtcc
     3781 ggtaacaccc attggcgggt tgttgtttat agcaggctgg ctggctttgc tgatggccgt
     3841 gcagcgaaaa cagcattaaa gcggcagtat cagtgtggtg actgtgcctt ttttattttc
     3901 gatatgataa ctccccccga tgctttccat ccggcgggcg atattcttca atccatttcc
     3961 gaattgtctc agcttatcca ggtcaattcc ttttccatta tcagcaatcc ggatacataa
     4021 ttcattaccg atgctgatat caatctccac caggctggcc tctgcgtgtt tcaatgtgtt
     4081 attcagggtt tctttcaggc atagaaatat attacgccgt ttatcacctg ccaattcttt
     4141 atcagggatc tgatcagggg ctttcagcct gcattccaca tttgttccgt caaaatattc
     4201 cagtgaataa gagcggatat aggacaccag gttatccaat gtgtcgtttc cgctgttcat
     4261 actccagata atcgcattca tcttattgag cacatcatct gccgaacggg agatcttctc
     4321 aatttcaatg ggtgtatttt ctttcatttt attccgggca atttcactca tcagccggat
     4381 cgcggtcatc ccggcgccca gttcgtcatg catatccgcg gagatccgct gccgttcttc
     4441 ctgctgtgct ttgtacacgg caatttcttt ttcgtattcc ttcagcaggt tctgcgcttt
     4501 cagggtttct ttttccttgg tttcattgat gatattgcgc ctgtttttat aattcaggcc
     4561 agccaggaat aacacgagtt ccaggaacaa acccagttca tagtagataa tggaggcgtt
     4621 cagcagtccg ggtaattttc tgaactgtgc atcatacaca atccctaatt gcgagatcgt
     4681 ggaaaagatg aacaggaaca ggttgcccca gaatagatac cgtaataatt tttctttcca
     4741 gttacggatg ctgtatacga ggtagacgag gatcaacgtc agtaataaaa ccttggaaag
     4801 tatttcaacg aggtattcca aaacataatt tcccgtgaaa taatggagga cagtataaag
     4861 caacatggag cccaccagta tggatatgcc tgtattatat aatttggaaa gaaagggatg
     4921 ggtttgatct gtgttcagga acttcttcat aaacaacata tagaaaataa ttcccgtggc
     4981 ctgtagcaaa aagtcaagat acccttcaat aaaaaagcca aaaggactgg catacagatg
     5041 gaatactgtt tgcaggaaaa gtaatccgcc cataaacaag gcatagccgg aataaaacag
     5101 gaactcctta ttgccgccct ggaagaaatt ggtgagcgag aacaggatca tcatcagtaa
     5161 caaaccgcaa aaaacatagg tgatcaggtt ttgctgatta tgtttaccgt gcagttgtgc
     5221 cacgaatgcg gtcacgtggt ctttgcctat aacccgcgga cgaagcaggt tgttataggt
     5281 tttaacacct gacaattctg taaccagtaa ggccgaatca tttggtccca gttctatcag
     5341 cctgaaacca tcccgttcat tgattgccgg ccggatgtca ggtaaaggga tcagctgatt
     5401 tccctccacc cggtaaagat taattttagt gaaatagtag cccgggcaga agtacacagc
     5461 actgttgctg tccgtgttgt tacaaacgtt gaaccggagg aaaattcgtt tggtgaccca
     5521 atcattgggc aaggtacctg aatgttgaat cccgggctca aaaaccatcc gggggtataa
     5581 ttcattgatg gctccctttt gcgtggtata aaaactgctg atgtccttgt taaaccgttc
     5641 ttccaggatt actttgctga tatccagata gacatgactg ctgtcctggg ccgtcaatga
     5701 acagggcagg agcagcaggc aaaaaaagca gacgtttgat atgaaaaagc gggaaaacct
     5761 catttggtca ttacctggcc aaatgtaagg tttcctgcct tctttttaat gaattgctcc
     5821 tgtttcggca cacggactgc tgctgatccg gtatcaagct tgccagattt gggataagag
     5881 gccaatcggg tggttttggg taaaggcata tcgcccgttg tgacaaacta tttatctttg
     5941 cagctatggc taataagctg aggaagaaat cgaaagatcg tgctgaacca acccctcctg
     6001 tgagtccgga cctgaaagaa ttcagaaagg aaaaagaaga agcggtggac tggaaagcca
     6061 ttgcccgtga tgaacgtacc tggaagatcg tgggggctgt tttcctcctg atttctatct
     6121 ttctctttat atcctttacc tcttattttt ttacctggaa agaagaccag gcggtggccc
     6181 aacaagggtt ttcagccctg ctcgatacgg atgaacccgt tgccaacctg ctgggcagac
     6241 taggggcggt aacttcccat ttttttattt ttaaagcatt cgggatagcg tccttcctga
     6301 tttgtacctt tttctttgta gtgggagtca acctgctttt ccgtcgcaaa gtattttcaa
     6361 tctggcggaa tctaaaatat gtaacccttg gtctcctgat tttgtcagtc agtctttctt
     6421 ttgttttcca gcataccgaa ttcgcatttg gcggggctgt tggcaacctg atcaatcaga
     6481 aactctatgg tgccgtaggg accatcggga cagccgccat tttgttattg ctggctaccg
     6541 gctattttat ctggcagttt aatccggcat tcaatctgcc acagaaaaaa caaccacaac
     6601 ccggtgcaga aactgccact gtgaatatca ctgaagaagc gactccggtt ttggctaccc
     6661 agacagtgaa tgacctgtat gcaggcaagg acaaagcaaa acctaattca ttaaaagggg
     6721 agagcccggt cgtgatcaat tttccggatg aagagccctt gcatgacctt aaaatggtcg
     6781 agaaagagct accccttgaa gtagtgcagc cccccgttca acagcaggaa tccattcctg
     6841 aaatagaagt ggtgaaggac ctgatgcata cacatgatct ggatataaca ctacctgaac
     6901 cggaagagga gatcattcaa aaacccaggg atttaactcc aacaaaaaaa ggagaccagg
     6961 agcttgaact cgagattaaa gcggtgccgg agaaagtgga agaagaagaa atagccgggg
     7021 tggagaacct gccgccttat gaacctaccc tggacctgcg tgactataaa tatcccaccc
     7081 tgaacctgct ggagaaccat ggaggggaaa aaatcgtaca ggataccagt gagctggaga
     7141 atcataaaaa ccagatcata gccaccctcc ggaattactc tattgaaatc cagaagatca
     7201 gtgctacgat cgggcccacg gtaaccttgt atgaaatcgt accggctcca ggcgtccgta
     7261 tttcccggat caaaaacctg gaagatgata tcgcgttgag ccttgccgcg cttgggataa
     7321 ggattatcgc gcctattccc ggtaaaggaa caattggtat tgaagtgccc aatatccgga
     7381 aaacgatggt gagtatgcgc agtctgctgg cctcggaaaa attccagaac aatgccttta
     7441 acctgccgat tgccattggt aaaaagatcg acaatgaaaa cttcatcgtt gatctgacta
     7501 ccatgcctca cctgttgatg gcgggggcta ccggacaggg taaatcagta ggggtgaatg
     7561 ccatcctggt ttccctgttg tataaaaaac acccttccca gttaaagctg gtgttgatcg
     7621 atccgaaaaa agtagagctc agtatctacc gtcgtattga gaagcacttc ctggccaaac
     7681 tgccgggcga agaggaagcg attattacgg ataccaagaa agtggtgaat accctgaatg
     7741 cgctttgtat tgaaatggat aaccggtatg acctgctcaa ggaagccggt tcccggaata
     7801 tcaaggagta caatgagaaa ttcatcaagc ggaaactcaa cccgcagaaa ggtcaccagt
     7861 acctgccgtt tattgtattg gtgattgacg agttcgccga cctgatcatg accgcgggta
     7921 aagagattga aatgccgatt gcgcgactgg cacagctggc ccgtgcggta ggtattcacc
     7981 tgattattgc cactcaacgt ccttctgtaa atattatcac cggtaccatc aaggccaact
     8041 tccccgcccg tatcggattt aaagtgtctt caaaaataga ctcacgtacc atcctcgata
     8101 cgggaggagc cgagcagctg atcggaaagg gggatatgct gatttcctat aacggggaac
     8161 ttacccgtct gcaatgtgct tttgtggata ctccggaagt ggatgaagtg gtggacttta
     8221 tcagcaacca gcgcggctat tcccaggcct tcctgttgcc ggaatatgtg gatgaaaaag
     8281 acctggaagg aaaagatttt gatatgggtg accgggattc cctttttgaa gatgccgccc
     8341 ggctgatcgt acagaaccag atcggttcca cctccttatt gcagcgccgg atgaagctgg
     8401 gctataaccg ggccggccgg ctcatggacc agcttgaggc agccggaat
//
LOCUS       DAOPMW010000113         8424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9354_length_8424_cov_10.821365, whole genome shotgun sequence.
ACCESSION   DAOPMW010000113 DAOPMW010000000
VERSION     DAOPMW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_8424_cov_10.821365"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="PLU11_07075"
     CDS             complement(<1..1738)
                     /locus_tag="PLU11_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58917.1"
                     /translation="MRKIILFLLFLQFQLCCFAQNRMLDSLKATLRNAKTDTARLRLY
                     VALGEASSKNDKWLYTEPALQLVDQLFLVEKDNQERQKLIGQEQSLLSLMGVYYRNSG
                     VTEWGKAMAYLQHRLQAIEKTGDQRRAGSFLYGMAGVSIDQKKDSALFRKYITRSISV
                     AKEIKDTVLMVNGYLFMGAFYSSAGNYEAGLDAIQSSIFSCREMSFDKGLIQSHRLLA
                     ILYRDFNESDLALENLQTALAISYKTKDSVEICLTLRNLGNFYARQKDITKAIENLEL
                     VQKINGSIGNPPFTSGDIYLDIGNAYSDNMDYKQAQFFLEKSLSTTEGLSDQKEKATD
                     NWRIPANLMHLGNVHYQQNHFEIASNYHTRCVRFLDSIGDPYQTKSYMLYLLARDYFG
                     LKDFARAKELSDIANVEFKRDWFDVEGRADIELLSFRIDSARGDGFGALSHYKEYASL
                     KERLSGDEIKREAQKDKFNAEKEKQKSEQDKKDAIARRTRNLQYVAIGAMLLLGVFLC
                     YGYIVKNRDKKKIEKAYADLQSAQSQLVQSEKMASLGELTAGIAHEIQNPLNFVNNFS
                     EVNSELISELVDE"
     gene            complement(1772..5389)
                     /locus_tag="PLU11_07080"
     CDS             complement(1772..5389)
                     /locus_tag="PLU11_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58918.1"
                     /translation="MKLTKQLKAEIADVYEAFWGALFYADIEMYSLFLDEDYRLIGTT
                     EQEFFFSKAEAVRFLKATADQLAGNLERRNSRHKIEIIDNLVFITDQFDAYVLIDDKW
                     SFYEKTRVTTLLQEKKDGWKIIQQHFSFPDNKAGSGQTIGLEKIAKENLQLKEAIRRR
                     TIELELKNKELEIEFSLERVRAVAMGMKQPADMLEVCEVITRELISLGVGPIRNIQTA
                     VIDTEKETYQNYEYYQVAREKLVTTVAYKEHPTIHAFVKRMVKDPEGFYARRMIGEGL
                     KKWIKYQAKAGQFVDPTSKKAASLHYYFYSIGSGALGITSYAPLSKEAIGLFKRFRNV
                     FQLAYARFIDIQQAEAQTREAQIEASLERVRSRAMAMYSSAELTEVALELRKQMGLLG
                     QKDLEVCAIHLYDDDHSFESWSAMKVPGSANEILQTQARFPKKGIRIVDELMKNYNEE
                     KKEYVIVNAGAKSVEWFSVLKEYAPELHSSIMQVIGDMPLRKLKSNWSVADFSGGALV
                     MVTYGEPDAASRSLLRRSANVFEQAYIRFLDLKKAEVQAREAQIEVALERVRSRSMAM
                     HKSEELADLSLELVKQVQALGVATWFCAFNIYEDDENGSVEWGSNGQGTFPKYRTPRE
                     GVFLRYYEAGQKGETLLINEIGEAECPAHYEYLCTLPGLGEQLLGMKNAGIAFPVSQI
                     DHVAFFKYGYIIFITYKAVPESHDIFVRFARIFEQTYTRFLDLKKAEAQAKEAQIEAA
                     LEKVRSRTMAMQSSNELQETAAVLFNEFKKLGTDNVYQVTIGIYNEEERLIDFRVTSW
                     AGSGAQENRSFKLDMNEPTVLKPAVDAWKANKKSLVIDLSGETLEGWLNYRNKMSGVA
                     VSSADTAGRRVITVAYYSKGHLSISTPVPVPTETQRILERFAAVFDSTYTRFLDLKKA
                     EAQALRAEQDLMAIKEAKQNAEEALAELQTTQKQLIQAEKMASLGELTAGIAHEIQNP
                     LNFVNNFSEVSKELLDEMKDAIDKGDTEEVKDIMNDVIQNLEKINHHGKRADGIVKGM
                     LQHSRSSSGVKEPTDINALADEYLRLAYHGLRAKDKSFNAALKTEFDDTIGNINVIPQ
                     DIGRVILNLITNAFYAVDEKKKQQPAGYDPEVTVTTRSQKSLSGDASVVISVKDNGNG
                     IPRHVIDKIFQPFFTTKPTGQGTGLGLSLSYDIVKAHGGELKVETTEGEGSGFIITLP
                     V"
     gene            complement(5392..7359)
                     /locus_tag="PLU11_07085"
     CDS             complement(5392..7359)
                     /locus_tag="PLU11_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58919.1"
                     /translation="MKKFSILVTLFIFFVATGKGQVSAERIQDMKQQLGVVTNDSLKM
                     FYLEQIAVGYRFSNMDSSVLYNDQALQLAVARGDMVKKCELLSLKGATMLETGLMAEA
                     LQLQLEALRQAEEIKADETRAIALNHIGNIYMELADYRKANDYYFQSRDIFKGTNNLV
                     YYNELSNIGNIYTLLGKPDSALFYQQIVLDMCGKSDNRHAMVRPEVMFRMGNAYRLNK
                     DYPKALEFYNRGIAEALFDNDIRNLTMNYNFLANLYREMNQQDSAFHNGYLAFNAASS
                     VLFRKGRYDASLLLSELYHKKGKPDSAYHYLSIATVEKEQLMGAERIRELQRILLNEK
                     EAQRQKEVQRIAEQNRQRQLLLLAGLGIFLLVAAILYRNNKVKQKSNRKLEKTLTDLK
                     ATQSLLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNTELISELVDEVEKGNTAE
                     VKLLATDIKGNSEKIKHHGKRADAIVKGMLQHSGRGSGKKEPTDINALTDEYLRLAYH
                     GLRAKDKSFNAQLHTDFDETIGAVNIIQQDIGRVILNLITNAFYAAPLPPKGGFSDPD
                     YRHIPTIWVSTKRIRDSVIISVRDNGPGIPEEIRDKIFQPFFTTKPTGQGTGLGLSLS
                     YDIVKAHGGEINVETKEGGGTTFTISLPVNQ"
     gene            complement(7363..8406)
                     /locus_tag="PLU11_07090"
     CDS             complement(7363..8406)
                     /locus_tag="PLU11_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN58920.1"
                     /translation="MNQQIQLILDYFQQQEQLSADEKNALIKALKKVKSDLEIAEFKL
                     ERTEKVKRTTAILLDETIQELELKRKAVEETNAALQQSMEELKAAQAQLIQSEKMASL
                     GELTAGIAHEIQNPLNFVNNFSDVTLELIGEMVEEVDKGNTEEVKAIARDVQQNMEKI
                     LHHGKRAGEIVKGMLQHSRTSSGQKELTDINALADEYLRLAYHGLRAKDKAFNATLHT
                     EYDENAGKVNVIPQDIGRVILNLITNAFYTVNERKRLKEEGYEPVVTVSTSRISDHIE
                     IKVKDNGMGIPKKVLDKIFQPFFTTKPTGHGTGLGLSLSYDIVKAHGGELSVHSDHGE
                     GTVFIVSIPTNHQ"
BASE COUNT         2028 a         2201 c         1599 g         2596 t
ORIGIN      
        1 cctcgtcaac caattcactt atcaactcac tatttacttc cgaaaaatta ttgacaaaat
       61 tcaacggatt ctgaatctca tgagcaatgc cggcagtgag ttctcccaga ctggccattt
      121 tctcggattg gacgagctgg gattgggcgg attgcaggtc ggcgtacgcc ttctcaatct
      181 tcttcttgtc cctgttcttt acaatatacc catagcataa aaaaacaccc agcagcaaca
      241 tggcaccaat ggcaacgtat tgcaggttac gagtacgcct ggcaatggca tctttcttat
      301 cctgttctga tttttgtttt tccttttcgg cgttgaactt atctttctgt gcttcccttt
      361 taatttcatc gccactcaat ctttctttca aggaagcata ctccttataa tgactcaatg
      421 cgccaaaacc atcaccccgg gcagagtcaa tcctgaaaga aagtaattcg atgtcagccc
      481 tcccttctac atcaaaccaa tctcttttaa attctacgtt ggctatatca cttaattctt
      541 ttgcccttgc aaaatccttt aacccaaagt aatcccttgc caacaaataa agcatatagg
      601 actttgtctg atacggatcc ccgattgaat caagaaaacg gacacaacgt gtatgataat
      661 tactggctat ttcaaaatga ttctgttgat aatgtacatt acctaaatgc ataagatttg
      721 caggtattcg ccaattatca gttgctttct ccttttgatc acttaaacct tcagttgtgc
      781 tcagactttt ttctaaaaaa aactgagcct gcttatagtc catattatca ctgtacgcat
      841 tcccgatatc cagataaata tcgcccgatg tgaacggggg attgccaata gaaccattga
      901 ttttttgtac tagttccagg ttctcaatgg cttttgtaat gtctttttgc cttgcataaa
      961 aatttcccag gtttcttaat gtcaggcaaa tctcaaccga atctttcgtt ttgtatgaaa
     1021 tagccagggc cgtttgcagg ttttccagtg ccaggtcaga ttcattgaaa tctctgtata
     1081 agatggccaa taatctatgg ctttgtatca accctttatc aaaactcatt tccctgcagc
     1141 tgaaaataga tgactggatc gcatccagtc ctgcctcata atttcctgcc gagctgtaaa
     1201 aagctcccat aaacaagtat ccgttcacca ttaaaaccgt gtctttaatt tcctttgcaa
     1261 cagatatgct tcgggtaatg tacttacgaa aaagtgctga atctttcttt tgatctattg
     1321 agacccctgc cattccatac aaaaaagatc cggctcttcg ctgatcgccg gttttctcaa
     1381 tagcctgcag ccggtgctga agataggcca ttgctttccc ccactccgtc acaccgctat
     1441 tcctgtaata aactcccatc agcgataaaa gtgattgctc ctgcccaatg agcttttgcc
     1501 tttcctggtt atctttttcc accaaaaaaa gctgatcgac caattgcaat gccggttcag
     1561 tgtacagcca tttgtcattc ttgctgctgg cctctcccaa tgccacgtaa aggcgcagcc
     1621 tggccgtgtc tgtttttgca ttccgcagcg ttgctttaag tgaatcaagc atcctgtttt
     1681 gtgcaaagca gcacaactgg aattgcagga ataataaaaa caaaataatt tttctcataa
     1741 cagtttacct gatattaatg tccccggtag ctcataccgg taatgtaata atgaatccgc
     1801 tgccttcgcc ttccgttgtt tccaccttca gctccccgcc atgcgctttc acaatatcat
     1861 aactcaggct cagtcccaat cccgttcctt gtccggtagg cttggtggta aagaagggct
     1921 ggaatatctt atcgatcaca tgccggggaa tgccattgcc gttatctttc acactgatca
     1981 ctaccgatgc atcaccagaa agagacttct ggctccgggt agtcacagtc acttccggat
     2041 cataaccggc aggctgttgc ttcttctttt catcaactgc ataaaacgca ttagtgatca
     2101 ggttcagaat cacccgcccg atatcctgcg ggatgacatt aatatttcca atcgtatcgt
     2161 caaattcagt ttttaatgca gcgttgaatg acttgtcttt ggccctcagc ccgtgatagg
     2221 ccagtcgcag gtactcatcc gccagcgcat tgatatccgt cggttccttc accccgctgc
     2281 tgctgcggct gtgctgcaac atccctttta caatgccatc cgctcttttg ccatgatgat
     2341 tgatcttttc cagattctga atcacatcgt tcatgatatc ttttacttct tccgtatcgc
     2401 ctttatctat agcgtctttc atttcatcca acagctcttt acttacctca ctaaagttgt
     2461 tgacaaagtt cagcgggttc tgtatctcat gggcgatacc ggcggtaagc tccccgagac
     2521 tggccatttt ttccgcctgg atcagttgct tttgagtggt ctgcagttcc gccagggcct
     2581 cctccgcgtt ctgtttggct tctttaatag ccattaaatc ctgctcagcc cgtaatgcct
     2641 gtgcttccgc ttttttcaga tcaagaaaac gggtataggt gctgtcgaat acagcagcaa
     2701 aacgttccaa tatccgttgt gtttctgtgg gcaccggcac cggggtagat atggacaaat
     2761 gcccttttga ataataggcg acagtgatca ctcttcgtcc ggccgtatcg gcactgctga
     2821 cggctacgcc gctcatttta tttctgtagt tcagccatcc ttctaatgtt tctccggaaa
     2881 gatcgatcac caaagatttt ttattggctt tccaagcgtc tactgcgggt tttaacacag
     2941 tcggttcgtt catatccagc ttaaaagaac gattttcctg tgcaccgcta ccggcccaac
     3001 tcgttaccct gaaatcgatc aatctttcct cttcattata aatgccgatc gtaacctggt
     3061 atacattatc ggtacctaat tttttaaact cattaaaaag cacggccgcc gtttcctgta
     3121 actcattact gctttgcatc gccatcgtcc ggctacgcac tttttctaac gccgcttcaa
     3181 tctgcgcttc tttggcctgg gcttctgctt ttttcagatc aagaaagcgg gtataggttt
     3241 gttcaaaaat tcttgcaaac cgaacaaaga tatcgtggga ttcaggtacc gccttatagg
     3301 taataaagat gatatacccg tatttaaaaa aagccacatg gtcgatctgg gaaaccggaa
     3361 aagcaatccc ggcatttttc atacccagca attgttcccc cagacccggt aatgtacaca
     3421 ggtactcgta atgggccgga cattctgctt ctccgatttc gttgatcagc agtgtttctc
     3481 ctttttgtcc tgcttcataa taacggagaa agactccttc ccggggtgtt ctgtattttg
     3541 gaaaagttcc ctggccatta ctgccccatt ccactgagcc attttcatca tcctcgtata
     3601 tattaaatgc acaaaaccag gttgccactc caagggcttg cacctgctta accagctcta
     3661 gagaaagatc ggccagctcc tcacttttat gcatagccat acttctggac cttactcttt
     3721 ccagggcaac ctctatttgt gcttccctgg cttgcacttc tgcttttttc agatccagaa
     3781 aacggatata ggcctgctca aaaacattgg ctgaccttcg caggaggcta cgtgacgctg
     3841 catcaggttc accataagtg accatcacca gtgctccccc ggaaaaatct gccaccgacc
     3901 agttggattt aagttttctc agcggcatgt ctccaatcac ctgcatgatg gaagaatgta
     3961 attccggagc atattctttc aacacgctaa accactccac actttttgct cctgcattta
     4021 caataacata ttcctttttt tcctcgttat aatttttcat cagctcatcc acgatccgga
     4081 ttcctttttt gggaaagcgg gcctgtgtct gcagaatttc attggccgaa cccggtactt
     4141 tcatggcact ccaggattca aaagaatgat catcatcata cagatgaatg gcacatactt
     4201 cgaggtcttt ctgcccgaga agtcccattt gttttctcag ctccaaggct acttctgtta
     4261 attccgctga actatacatg gccatggccc ggcttcttac tctttctaac gaagcttcaa
     4321 tttgcgcttc ccttgtctgg gcttctgcct gctgtatatc gataaacctt gcatacgcca
     4381 actgaaagac attccggaaa cgtttgaaca aaccaatcgc ttcctttgaa agtggggcat
     4441 acgaggtgat gcccagtgcc ccggacccga tggaataaaa atagtaatga agggaagcgg
     4501 ctttctttga cgttggatcc acgaattgcc cggcctttgc ctggtacttt atccatttct
     4561 tcaatccctc ccctatcatc cttcgggcat aaaacccttc aggatccttc accatccgct
     4621 ttacaaaagc atgaatggtc gggtgttctt tataggcaac tgtagtcacc agtttctccc
     4681 gggctacctg gtaatattca taattctggt aggtttcctt ttctgtatca ataaccgctg
     4741 tctgaatatt ccggatgggc ccgacaccca gactgatcag ctcccgggtt atcacctcac
     4801 aaacttccag catatcggct ggctgcttca tacccatggc cacagcccgt acgcgttcca
     4861 gcgagaattc aatctccagc tccttgttct ttaactccag ttcaatggtt cgtctccgga
     4921 tcgcttcttt gagctgcaga ttctctttgg caatcttttc aaggccaatg gtctgtccgc
     4981 tcccggcctt attatccgga aaggagaaat gctgctgtat gatcttccag ccatccttct
     5041 tctcctgaag cagggtggtt acccgggtct tttcataaaa actccattta tcgtcaatga
     5101 gtacataggc gtcaaactga tcggtaatga aaacaaggtt gtcaattatt tctattttat
     5161 gacggctgtt tcgtctttcc aggttaccag ccaactggtc agcggttgcc tttaaaaaac
     5221 gaacagcttc tgccttgctg aagaaaaatt cctgctcggt tgtaccaatc aaccggtaat
     5281 cttcatccag gaaaagactg tacatctcta tatccgcata aaacaaggca ccccaaaagg
     5341 cttcatacac gtcggctatt tctgctttca gttgtttggt cagcttcatc tttattgatt
     5401 aacaggtaaa ctgattgtaa aagtggtccc accgccctcc ttggtctcta cattaatttc
     5461 cccaccatgc gccttcacaa tatcataact cagactcaat ccaagtcctg taccctgccc
     5521 agtgggcttg gtggtaaaga aaggctggaa tattttgtcc ctgatttctt caggaatacc
     5581 aggtccgtta tccctgaccg aaataattac gctatccctt attctttttg tactcaccca
     5641 gattgtggga atgtgcctgt aatcggggtc tgagaacccc cctttggggg gcaggggggc
     5701 cgcgtaaaag gcattggtaa tcaggttcag gattacccgg ccgatatcct gttgaataat
     5761 attgacagcc ccgatggtct catcaaaatc tgtatgtaat tgggcattga acgatttatc
     5821 ctttgcccgt aatccatgat aagccagtcg caaatactcg tcggtcaacg cattaatatc
     5881 cgttggttct ttcttcccgc tgcccctgcc ggagtgttgc aacatccctt ttacaatagc
     5941 atccgctctt ttaccgtgat gttttatttt ttcagaattt cccttaatgt cagttgctaa
     6001 caactttact tcggctgtat tccccttttc aacctcgtca actaattcac ttatcaactc
     6061 cgtgttcact tccgaaaaat tattcacgaa attcaacggg ttctgaatct catgggcaat
     6121 gccggcggtg agttcaccca ggctggccat tttctccgac tggatcagca gggactgagt
     6181 ggccttcaga tcagtcaggg ttttctcgag tttccggttg gatttttgtt ttactttatt
     6241 attacggtac aaaatggcgg ccaccaataa aaaaataccc aggccggcca acaacagtaa
     6301 ttgccgttgc ctgttttgtt cggcaatacg ctgcacctct ttttgccgct gcgcttcctt
     6361 ttcgtttaac agaatccgct gcaattccct tatccgttct gcgcccatca gttgctcctt
     6421 ttctactgta gcgattgaaa ggtagtgata ggcactgtct ggctttccct ttttatgata
     6481 caattcactc agcaacaagg aggcatcata ccgccctttc ctgaacaaca ctgaactggc
     6541 agcgttgaat gccaggtatc cattatggaa agctgaatcc tgttggttca tttcccggta
     6601 caggttagcc agaaaattat aattcatcgt cagattcctg atatcattat caaataatgc
     6661 ctctgcaatc ccgcgattat aaaattccaa cgcttttgga tagtctttgt tcagccggta
     6721 cgcgttcccc atccgaaaca tcacttcggg acgcaccata gcatgcctgt tatctgattt
     6781 cccacacata tccagcacga tctgctgata aaagagtgct gaatccggct tcccgagcaa
     6841 ggtatagata ttaccgatgt ttgaaagttc attataataa accagattat ttgttccttt
     6901 aaaaatatcg cgtgactgga aataatagtc attcgctttc cggtaatctg ccagttccat
     6961 atagatatta ccaatatggt tcaatgcaat tgcccgggtc tcatctgcct ttatttcttc
     7021 tgcctgccgt aatgcctcca gttgcaactg cagggcttcc gccataagtc cggtttccag
     7081 catagttgct cctttcaggg acaagagttc gcactttttt accatatcac cccgggccac
     7141 tgccaattgt aatgcctggt cattataaag cacggaggaa tccatattcg agaaccggta
     7201 tcccaccgca atctgttcca ggtagaacat cttcaaagaa tcattggtta ccactcccag
     7261 ttgttgcttc atatcctgga tacgttccgc agacacctgt cctttgcctg tggcgacaaa
     7321 aaaaatgaaa agggtcacta aaatgctaaa cttcttcata ctttactgat gattagtcgg
     7381 aatggaaacg ataaaaactg tcccttcgcc atgatctgaa tgaacggata attccccgcc
     7441 atgtgctttg acaatatcat aactcagtga taagcccagc cctgttccat gccccgtagg
     7501 cttggtggta aagaagggtt ggaatatttt atccagcact tttttcggaa tgcccatacc
     7561 attatccttc acttttattt ctatatgatc tgaaattctg ctggtactga cggtgaccac
     7621 cggctcatac ccctcttctt taagcctctt ccgttcattc accgtataaa aagcatttgt
     7681 aatcaggttc agaattaccc ggccgatatc ctggggaatc acattcacct ttccggcatt
     7741 ttcatcatac tccgtatgta aggtggcatt aaaggctttg tccttggcac gcagcccatg
     7801 ataggccagc cgcaaatact catccgccaa tgcattgata tcagtgagtt ctttttgtcc
     7861 gctactggtc cggctgtgct gcaacattcc ttttacgatt tccccggccc gctttccatg
     7921 gtgcaatatc ttctccatat tctgctgcac atccctggct atcgctttta cttcctctgt
     7981 attccctttg tcaacctctt ccaccatttc accaatcaac tccagggtta catcactgaa
     8041 attgttgaca aaattcaacg ggttctggat ctcatgggcg ataccggccg tcagttcacc
     8101 caacgaagcc attttctcag actggatcaa ttgcgcctgt gccgctttca gttcttccat
     8161 ggattgctgc agagcggcat tggtttcctc gaccgctttc cgtttcagtt ccagttcctg
     8221 gatcgtttca tccagcagga tggccgtggt gcgcttcact ttctctgtac gctccagctt
     8281 gaattcggcg atctcgagat cacttttaac tttcttcagc gcttttatca atgcgttctt
     8341 ttcgtcagcc gataactgtt cctgctgctg aaaataatcc agtatcaact gtatctgctg
     8401 gttcatttat ttttctttta aggc
//
LOCUS       DAOPMW010000114         8404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9379_length_8404_cov_6.468679, whole genome shotgun sequence.
ACCESSION   DAOPMW010000114 DAOPMW010000000
VERSION     DAOPMW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9379_length_8404_cov_6.468679"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1871
                     /locus_tag="PLU11_07095"
     CDS             132..1871
                     /locus_tag="PLU11_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN58921.1"
                     /translation="MNRKITRLLLPLFLTNLALNAQVMNKMPAVPNPKPVDSVLMGDW
                     KSFPELKLNLPVTPGPFEPTWESIEKNYPGEPAWLREAKFGIWVHFGPQSAGESGDWY
                     ARRMYVQGSKANNNHLNKYGHPADVGYKEVLRDWNPIKLDPAKLTKIYRDAGAKYLMI
                     QVVHHDNYDLWNSKYQPWNSVNIGPKRDLLGEWAKACRADGMRFGVTFHHEYSWWWWQ
                     TAFGSDSSGEKKGVPYDGTLTLADGKGKWWEGYDPKMLYGIDLREYKGVEQKAHQDWS
                     PAPAGIFINHLDYAKWYATNWALRMMDVVEHYDPDFIYTDGTVQGPFTGYGTGTGIKA
                     DAMQHVMADFYNRTLKRRGLVNTFSIVKFRNKTNGTVNTEEFGIPSSINTYQPWIGEA
                     PVGDWFYAPGFTYNSDMMIRYVIEAIARDGNAALCISILPDGSLDEGSLKMLNEVGNW
                     MRMNGEAVYGSRAWKIPGEGEQINGKLKMLPGGALNRKQAEFSFSTMDFRFTTGKNNS
                     LYAFCMTVPAPGTRLVIRSIGTDSAFLGKPVKQVSLLGHKGKLKWKQEKDGLMIICPA
                     KMMYKTAIVFKID"
     gene            1894..4106
                     /locus_tag="PLU11_07100"
                     /pseudo
     CDS             1894..4106
                     /locus_tag="PLU11_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
     gene            4182..4508
                     /locus_tag="PLU11_07105"
     CDS             4182..4508
                     /locus_tag="PLU11_07105"
                     /inference="COORDINATES: protein motif:HMM:NF028447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HPN58922.1"
                     /translation="MQYTGEKPNDTLEIRIYTGADGTFSLYEDEGDNYQYEKGMYTII
                     PFHWHEKKQTLTIGTINGSYPGYLKRRAFSVVFISEGNISAAYPGHKNKMVFYNGKKI
                     TLINQL"
     gene            4622..6538
                     /locus_tag="PLU11_07110"
     CDS             4622..6538
                     /locus_tag="PLU11_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPN58923.1"
                     /translation="MILLCQPMVQAQVPPGNAKKISPDLFGLFFEDINYAADGGLYAE
                     LVQNRSFEYNPTERKEWHPLYNWDYFTSGYSYGNVNIETSQPVHPNNPHYTVLEAEHI
                     YQQDGSVGIKNQGFDGIVVKAGNLYQFSVFAKQLSIQPVTLKISLQNRKGEVLAEQNL
                     TVSSIQWGKQTAVLQPSADYDTASLVIALVSKGKIALDVVSLFPEKTFKKRPNGMRAD
                     LAQLLADMKPAFIRFPGGCLVHGDGLGNMYRWKNTIGPLEQRVEQRNIWGYHQTGGLG
                     YYEYFQFCEDIGAKPLPVLPAAVSCQNSGGTWRIGGTGQRALPLEDMQDYIQEVLDLI
                     EWANGPVNSTWGSKRAAAGHPATFNLQYIGIGNEEKITPEFEQRFKLVHDAVKARYPE
                     ITVIGTSGPFHSGEDFDKGWAFAKNQQVKVVDEHYYVQPEWFISNQYRYDNYPRSSGN
                     VYLGEYASWGNQLKNALAEAAFMTGLERNGDVVQLASYAPLFAKKNNTQWKTDMIFFD
                     NTRYYLTPNYHVQKLFSVNKGDRYLNNIIQKEDRDTSIAASATGDSRTGDIILKLVNT
                     GKDKKTIATDLTGYKYSSDGVKILLIGTADQQNSFSDPDAVKPDQGPIAIKKKMNIEL
                     PPMSLMVIRIKTLK"
     gene            6585..8108
                     /locus_tag="PLU11_07115"
     CDS             6585..8108
                     /locus_tag="PLU11_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HPN58924.1"
                     /translation="MTWQTGNAQNPVIYADVPDLSMIRVGNTYYMSSTTMHMSPGVPI
                     MKSTDLVNWKIINYAYDTLADVDALNLNNGKSAYGRGSWASSIRYHKGSYYVSTFSST
                     TNKTYIYTTKNIEKGPWERSSFNPSYHDHSLFFDEDGRVYLLFGNGKLGLVELENDLS
                     GAKEGGIHKVVIENASLPAGTNIGLGAEGSQLIKKDGYYYLFNITWPRGGMRTVVVHR
                     AQQLTGPWEGRLGLQDLGVAQGSLIDLPDGRWFAYLFRDYGSVGRIPYLVPVRWENGW
                     PVFGKNGKIPEALGLPVSKGLIPGIVSSDEFTRKKKNRALPLVWQWNHNPDNRFWSVT
                     ARKGFLRLTTGRTDSLFVRSRNTLTQRTFGPLCSGSTMIDVSGMKEGDVAGLAAFQRK
                     FGQIGVKMKDGKKYLFMTNAESNTPVEIQTIPLLQSAVHLKIECDFRIKTDKAYFFYS
                     LDGIKWEPIGNTLHMEYTLMEHFMGYRFALFNYATKEAGGYTDFDFFHISDQISQHE"
     gene            8101..>8404
                     /locus_tag="PLU11_07120"
     CDS             8101..>8404
                     /locus_tag="PLU11_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58925.1"
                     /translation="MNKHIRTYPPYSRLPWLLLFLVLFATSHAQNPLIRTQFTADPSA
                     RVFGDKVYIFPSHDILATDGKGRKGWFCMEDYHVFSSSNLTEWTDHGMIITQNNVPW"
BASE COUNT         2580 a         1821 c         1896 g         2107 t
ORIGIN      
        1 gaatatgaaa tatggtatgg gaacagttca gctataacag atttaaaaat gatccgtata
       61 aacattttat aagaattaat aaaaaaagcc caaacatatc gactacatga ttaatagtta
      121 aaggatttga aatgaaccgt aaaataacac gcttactact ccccttattc ctgacgaatt
      181 tagcattgaa tgcacaggta atgaataaaa tgccagctgt gcctaaccct aaaccagtgg
      241 atagtgtgct gatgggagat tggaaatcat ttccggaatt aaaactcaac ttaccggtta
      301 cccccggacc ttttgagcca acctgggaat cgattgaaaa aaactatccc ggcgaacccg
      361 cctggcttcg ggaagccaaa tttggcatct gggtccattt tggtccgcaa tccgcaggtg
      421 aaagcggcga ttggtatgca cgtcgtatgt acgtgcaggg ctcaaaagct aacaacaatc
      481 atctaaataa atacggccac cctgctgacg tgggctataa agaagtgctg cgtgattgga
      541 atcctataaa gctggatcct gcaaaactga ccaaaattta tcgcgatgca ggtgctaaat
      601 atctgatgat acaggtcgtg catcatgata attatgattt gtggaattca aaataccagc
      661 cctggaattc ggtgaatatt ggccctaaaa gagacttatt gggtgaatgg gccaaagcct
      721 gtcgcgcaga cggaatgcgt tttggagtaa ccttccatca tgaatacagc tggtggtggt
      781 ggcaaacggc ttttggcagt gattcatccg gcgaaaagaa aggtgttcca tacgacggaa
      841 cccttaccct ggcagatgga aaaggaaaat ggtgggaagg ctatgatccg aaaatgcttt
      901 acggaataga tttgagagaa tacaaagggg tggaacaaaa agcacatcag gactggtctc
      961 cagcccctgc tggcatattt atcaatcacc ttgattatgc aaaatggtat gccaccaact
     1021 gggccctccg gatgatggat gtagtggaac attatgaccc ggatttcatt tacactgatg
     1081 gtactgttca ggggccattc accggatatg gcaccggtac aggaattaaa gcggatgcca
     1141 tgcagcatgt gatggctgat ttctataacc ggaccttaaa acgccgtggg ctggttaaca
     1201 cattcagtat agttaaattc cgcaacaaaa cgaacggcac cgtgaatacg gaagaatttg
     1261 gtattccttc atctataaat acgtatcagc cctggattgg tgaagcgcct gtaggagact
     1321 ggttttacgc acccggattt acctataatt cagacatgat gatccgttat gtgattgaag
     1381 cgattgccag ggatgggaat gctgcgctct gcatatccat cttgccagat gggtcgctgg
     1441 atgaaggcag ccttaaaatg ctgaatgaag taggtaactg gatgcgcatg aatggcgaag
     1501 cggtctatgg cagccgtgca tggaaaattc cgggtgaagg tgaacagata aatggaaaac
     1561 ttaaaatgct gccgggagga gcattaaacc ggaagcaggc cgaattcagt ttctctacta
     1621 tggattttcg ctttactacc ggcaaaaaca attccctgta tgctttctgt atgactgtac
     1681 cagcccctgg tacccggtta gtcatccgat caataggaac ggattccgca tttctcggca
     1741 aaccggtgaa gcaagtgtcc ttactaggac acaaaggaaa actgaaatgg aagcaggaaa
     1801 aagatgggct gatgattatt tgccccgcaa aaatgatgta caaaaccgca attgtattta
     1861 aaattgatta gtcagtaaca gcgattataa aaaatgagga taaaatcatt catactactt
     1921 tttctgatac catctctggc agccggtcag tcatataaac aatatagact tctgaaggac
     1981 cggctagtca ttgagcttac tgaagggact atgaatataa ttcccctttc agctaaagcg
     2041 attagaatac aatgggaaaa agggctccaa aaagaagaaa atgagcttat tcttaacagg
     2101 ccccttcaaa tccaacctgt tagtttttca gaaaacgact cttctttaaa actgagtact
     2161 gcttccataa cggtcagttt caacaaacag aatctggcaa tactcttcac cgacaaatcc
     2221 gggaaaattt tcctgagtga aaaagccgga agccgcaaac taacagacac aaaaaccgga
     2281 agtaccgcct gctacattgc agaacaggga tttatttcac cggaagatga atctcttttt
     2341 ggccttggac agttccagga cggccatttt aatctcagaa atatatcccg aaaattagtt
     2401 caggtaaata cccagatcgc catccctttt ttatattcaa gtaagggtta tggtatctta
     2461 tggcaccagt atggcctcac ccattttaat ccggccgata acacaatttt actcaccaaa
     2521 aaagacagtc tgacaagtga aacggggatg acagaagtaa ccaccacatc cggcacacaa
     2581 aaagtcagtc gtcagcaatc tgtatataca ggaaaatttt ctgtcgaaca ggatggcacg
     2641 tatttcatga tgctggacat gggaagtatg gaaagccggc atttgcttat aattgatggc
     2701 acaccagtca tcgatcagtc taatctttgg ctccctcctg cagcaggtat gcctgttaca
     2761 ctaaaagccg gggaacatat agtacaggtg gtatgcaaaa ctacaaatac gccttccctt
     2821 tcatggcgtt taactacccg tgaaataata ctccgttccc caaatgcaaa atcacttgat
     2881 tatgtggtgt tttatggaaa aagtgctgat gaagtaatcg gagcgtacag aaatctatca
     2941 ggcaatgtac ccatgctccc gctatgggcc tatggattct ggcaatgccg tgaacggtat
     3001 acttctggca aacaccttgt tgaaaccgat aagaattcag aaaaagaaaa ctaccggtag
     3061 atgtcatcgt ccaggactgg cagtactggg gcaaacatgg atggggggtt ccgcagtttg
     3121 atacaaccaa ctatcccaac ccggagggct tcataaatga actacataat ctgcatgcta
     3181 agttctccat ctctgtatgg gaaaacatgg ataaaaaatc atcagtggca caagaatatg
     3241 tttcaaaaaa tctttatata ccaaacagcc catggatcga catctttaat cctgagacac
     3301 aaaaaacgca ttggaatgtg ttaaacaaaa atctgttccg cctgggagtt gacagctggt
     3361 ggatggatgc cactgaaccc gaaaatgatg cattaacagg caaacaaacc tatttaggat
     3421 caggtgacct ttaccgcctg acctatccgt tatttgtaag taaagcagta tatgagggtc
     3481 aacgaaatac tgatccaaca aaacgggtgg tgatcctgac ccggtctgca tttgccggac
     3541 aacaacgata tggcaccatt aactggtcag gggatatcgg gtggaactgg gatagttata
     3601 aaagacagat tgtggcgggt cttcactaca acttaaccgg catgccttac tggacaacag
     3661 atatcggtgg tttcttccgc cctggcaatg gccaatatac cgatcctcaa taccatgata
     3721 tactcacccg ctggtttcaa tggggcgtat ttaacccgat ctttcgcata catggatatc
     3781 agaccgaaac tgaaccctgg aaatacgggg aacaggtgga aagcaacatg agaaaaatga
     3841 tgaatctacg gtaccgtctg ctgccctata tttattcttc cgcatggctt gtttcacaaa
     3901 aaggctctac tctgatgaga ccacttgtta tggatttcag tgaggatccg ctggccgttt
     3961 cacaggctta tcaatatatg tttgggaaaa gcattctcgt agcccctgta actgctccga
     4021 acactaatac ctgggatgta tatctaccca aatcaaccgc ctggtatgac ttctggacag
     4081 acaaaaagta tatgggtgga cagtccgtta aagcacccgc gccaaccgat aaaattccac
     4141 tgtttgtaaa agcaggagcc attattccgt ttggtaaaga aatgcagtac acaggggaaa
     4201 agccaaacga caccctcgaa atcagaattt acaccggtgc cgacggtaca ttcagtctgt
     4261 atgaagatga aggggataac tatcaatatg aaaaaggtat gtataccatt attccctttc
     4321 attggcatga aaaaaaacaa acattaacga tcgggacaat aaacggaagt taccccgggt
     4381 atttaaaaag aagagcattt tcagttgtat ttatcagtga gggcaatata tccgctgctt
     4441 atccgggtca caaaaataaa atggtttttt acaacggaaa aaaaataacc ctgataaacc
     4501 aattgtaagc cttgaaaaat caatatatca tctcagtatt tcttaacaaa tgaatgcaaa
     4561 aacaaaaata acctcaatat tatacaacaa aacaaccagt cgtctgatta ttttgacagc
     4621 tgtgatacta ctctgccaac ccatggttca ggctcaggta ccacccggaa atgccaaaaa
     4681 aatcagcccg gatctgttcg gccttttctt tgaagacatc aattatgcag ctgacggtgg
     4741 tttatatgcc gagctggtac aaaaccgttc ttttgaatac aatccgacgg agagaaagga
     4801 atggcacccc ttatataatt gggattattt caccagcgga tattcttatg ggaatgtgaa
     4861 tattgaaacc agccaacctg ttcaccccaa taatccgcat tataccgtat tggaggctga
     4921 acatatttat cagcaggatg gaagtgtggg catcaaaaat caaggctttg acggcatcgt
     4981 ggttaaagcc ggcaatttgt atcagttttc agtatttgca aaacaactca gcattcagcc
     5041 ggtcacctta aagatcagtt tgcaaaacag gaagggggaa gtacttgcag aacaaaatct
     5101 gactgtttct tctatccagt ggggaaagca aacggcagtg cttcaaccat cagcagacta
     5161 tgacacagcc tcgctggtga ttgccctggt cagcaaaggc aagattgcgc ttgatgttgt
     5221 ttccctattt cctgaaaaaa cattcaaaaa aagacccaat ggtatgcggg ccgacctggc
     5281 acaattgtta gctgatatga aaccggcctt tatacgtttt ccaggaggct gcctggtgca
     5341 tggtgatggc ttagggaata tgtatcgctg gaaaaacacc atcggtccgc ttgaacaacg
     5401 ggtggaacaa cggaatatct ggggctacca ccagacaggc ggattgggct attatgaata
     5461 cttccagttc tgtgaagaca ttggtgctaa acctttgcct gtattaccag ccgctgtcag
     5521 ttgtcagaat tcaggaggca cctggcgtat cggcggtacc gggcaaagag ccttacccct
     5581 ggaagatatg caggactata tacaggaagt tctggatctg attgaatggg caaacggacc
     5641 cgtgaactcc acctggggca gtaaaagagc tgcagcaggt catccggcta ctttcaatct
     5701 gcaatacata ggtataggta atgaggaaaa aatcacgccg gaatttgaac aacggtttaa
     5761 actggtccat gatgcagtta aagccaggta cccggagata acggtgatcg gtaccagcgg
     5821 acctttccat tccggagaag attttgataa aggatgggcc ttcgcaaaaa atcaacaggt
     5881 aaaggtcgta gatgaacatt attatgtaca accggaatgg tttatctcta atcaataccg
     5941 gtatgacaat tacccccgga gttccggtaa tgtatacctt ggtgaatatg cttcctgggg
     6001 caatcagttg aagaatgcgt tagctgaagc tgccttcatg acagggcttg aacggaacgg
     6061 agatgtggta caattagctt cttatgcgcc tttattcgca aagaagaata atactcaatg
     6121 gaagaccgac atgatcttct ttgataatac ccgttactac ctgacgccga attaccatgt
     6181 gcagaaattg ttcagtgtga ataaaggcga ccggtacctg aacaatatca tccaaaaaga
     6241 ggatcgtgat accagcattg ccgcctccgc aaccggagat agccgaacag gagatattat
     6301 cctcaaactg gtcaataccg gaaaggacaa gaaaacaatc gcgacagatc tgacaggata
     6361 taaatattca tcggacggag tgaagatact actgatcggg actgcggatc agcaaaattc
     6421 attcagtgat ccggatgcag taaaaccgga tcaagggcca atagccataa agaaaaaaat
     6481 gaatattgag ttaccgccca tgtcgcttat ggtgatccgg atcaaaacac ttaaatgagt
     6541 aatagaaaat gagatcgctt tttttgctga tactaacaat acaagtgaca tggcagaccg
     6601 gtaatgccca gaaccccgtc atttatgccg atgtaccgga tctctccatg atccgggttg
     6661 gtaacactta ttacatgagc agtacgacga tgcatatgag tccgggtgta cccattatga
     6721 agtcaaccga cctggtcaac tggaagatta tcaattacgc ttatgataca ctggcggatg
     6781 tggatgcact gaacctgaac aatggcaaaa gcgcttatgg ccgcggctcc tgggcaagct
     6841 cgatccgata tcacaagggc tcttattatg tgtctacctt ttcatcaacc accaataaga
     6901 cctatatcta taccaccaaa aatattgaaa aaggcccctg ggaaagaagt tcatttaacc
     6961 cctcctatca tgatcactcg cttttctttg atgaggatgg ccgtgtttat ctattattcg
     7021 ggaacgggaa actcggcctt gttgaattgg agaatgattt gtccggtgct aaagaggggg
     7081 gaatacataa agttgtaatt gaaaatgcca gtctgcccgc cggcaccaat atagggctgg
     7141 gtgctgaagg ttcacaactc ataaagaaag atggttatta ttacctgttc aatatcacct
     7201 ggcctagagg cggtatgcgc accgtggtcg tacaccgggc acaacaactc acaggcccat
     7261 gggaaggacg gttgggatta caggatcttg gcgtggcgca gggcagtctg atcgacttac
     7321 ctgatggccg atggtttgct tacctgtttc gtgattatgg atcggttgga cgtattccct
     7381 atctcgtacc agtacgatgg gaaaatggct ggcctgtttt tgggaaaaac ggcaagatcc
     7441 cggaagcact gggtttacct gtttcaaaag gacttatacc agggatcgta tcctccgatg
     7501 agtttacccg taaaaaaaag aaccgggcat tgcccctggt ctggcagtgg aaccacaatc
     7561 cggataaccg cttttggtct gttacagcac gcaagggttt tctgcggctc accacaggac
     7621 gaacggattc cctgtttgtc agatcaagaa acaccctgac acaacggact ttcgggccgc
     7681 tgtgttcagg cagtacaatg atcgatgtat ccggtatgaa agaaggcgat gtggccgggc
     7741 tggccgcttt tcaacgaaaa ttcggacaaa taggggtaaa gatgaaggat ggaaaaaagt
     7801 atctctttat gacaaatgcc gaatccaata caccagtaga aatacaaacg attcccctgc
     7861 ttcaatcggc tgtgcacctt aaaatcgaat gtgatttccg gattaaaaca gacaaagcct
     7921 attttttcta cagtcttgac ggaataaaat gggaacccat cggcaacaca ctgcacatgg
     7981 aatacaccct gatggaacat tttatgggtt atcgatttgc cttattcaac tatgcaacca
     8041 aagaagccgg cggctatacc gattttgatt tctttcatat cagtgatcaa atcagtcaac
     8101 atgaataaac atatcagaac ataccctcca tactcccggc tgccgtggct gttgcttttc
     8161 ctggtgctat ttgcaaccag tcatgctcag aatccgctta tccgtacaca gtttacagct
     8221 gacccttcgg ccagggtctt tggtgacaaa gtctatattt tcccctccca cgatatttta
     8281 gccacggatg gaaaaggacg aaaaggctgg ttctgcatgg aagactacca tgttttttct
     8341 tcctccaatc tgaccgaatg gacagaccat ggtatgatca tcacacaaaa caatgttccc
     8401 tggg
//
LOCUS       DAOPMW010000115         8392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9399_length_8392_cov_6.519491, whole genome shotgun sequence.
ACCESSION   DAOPMW010000115 DAOPMW010000000
VERSION     DAOPMW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_8392_cov_6.519491"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1518
                     /gene="mnmH"
                     /locus_tag="PLU11_07125"
     CDS             382..1518
                     /gene="mnmH"
                     /locus_tag="PLU11_07125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPN58926.1"
                     /translation="MAVQRIHIDEFLKLAETHPVFDVRSPGEYNHAHMPGAISLPLFT
                     DEERKQVGTAYKQISREQAIKIGLDYFGPKMRKMVEEVEAVSSRQLADSSRQAAVGSL
                     PTANCILVYCWRGGMRSGAVSWLLDMYGFKVYTLIGGYKKFRNHVLDTFKKPYRFNII
                     GGYTGSGKTELLKTLKEKGEKVIDLENIANHKGSAFGAIGLPPQPTQEMFENLLSNEL
                     SAISSKEGLRTTNYEQLTSCIWLEDESQRIGLINIPGDLWKNMRQSKVWFLDIPFEER
                     LKHIIPEYGILEQEKLIAAIERISQKLGHLNAKTAILLLKEGKISESFDILLRYYDKF
                     YFRSLHNREGLETLLHTINCKSVSPENADLLIREATAQSQTPLA"
     gene            1463..3097
                     /locus_tag="PLU11_07130"
     CDS             1463..3097
                     /locus_tag="PLU11_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58927.1"
                     /translation="MPISLSVKPLLKARHLWLSFLFLLAPLFFYSQTLTGLWVGALHN
                     DSSSVRKDQSFEIALTEYRGKVFGYSRSEFIVNDTLYYIVKRVKGTIEGDICEVKDDE
                     IISYNFRGKLDKGIKVTSTFRRNQTDSTWQLDGSWKTNATKKYYSVTGKVNLAEEKDL
                     TASKLFPHLEELNKADEMVFYKERNEPAPFVKVAKPERIQSEYTEKADILTKNEEKSL
                     SVKKPELPQPAQPVVPEVAVQTVPETKTVPVLPADALPGKNPVATNNPAQETAVVKNE
                     PVLTVVPDKKIAEPKPGDKLTAETNIPVVAAKTETPPIRTAAPANSANETANTNTANP
                     KVAAVKPVPVTNPVVKTQPTAPPVTAPTVKPGPTQDEKAVAKTKPTATAPALTPVSNK
                     TVISNPVETNLPKPRVIDPTALLVKKSVEDPIKKSTMIAGRKSEFSQEVYFKSDSLIL
                     TLYDNGEIDGDTVSVYLNGEVVMPSQGLKSSAIRKIIHMDDGLETFTLVMFAESLGKY
                     PPNTGLLVIRDGNDVYNLRFSSDFQKSSGIVFRRKQ"
     gene            3107..3889
                     /locus_tag="PLU11_07135"
     CDS             3107..3889
                     /locus_tag="PLU11_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPN58928.1"
                     /translation="MKLSGLLVFLLIHTICLSQTTRIDTVYSTQVKDKFVITVRKPAG
                     YSDTKKYHHVYMTDGSLGIADYVFGKEKSWAATIPNSCVIIAISHIGDWEEKRRRDFI
                     PSDITKNVEANFGNANTFYQFMKKELIPRIEKSMPNKKGRAFIGHSFGGLFCLYTLLQ
                     EDKLFDKHFAISPSVWANYNELDKIEAQYAKNHKALKADITIYVGGLEFLNKVLYSTR
                     NFYNTVSGRKYTGCWIRKNEIGNANHFSIRKPAIDRIFESLK"
     gene            3947..5005
                     /gene="selD"
                     /locus_tag="PLU11_07140"
     CDS             3947..5005
                     /gene="selD"
                     /locus_tag="PLU11_07140"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPN58929.1"
                     /translation="MTQDPVRLTQFSHGAGCGCKISPKVLDEILKTSVAFPDNDKLLV
                     GNHSRDDAAVYDLGNGTAIISTTDFFMPIVDDPFEFGRIAAANSISDVYAMGGKPLMA
                     IAVLGWPVNKLPADVAQKVVEGGRSICLEAGIPLAGGHSIDSPEPIFGLAVTGILPIE
                     NLKQNNTAKEGDLLFLTKPLGVGILSTAQKRGLITDDLLAVIIKQMTTLNKTGEELGK
                     IKGVHAMTDVTGFGFLGHLIEMAEGSGLSAEVWYDKIPAIPGVKEYIAQGVFPDGTTR
                     NWSSYGEKVKFEKGTPVAEAFKLLPDPQTNGGLLIAIAPETKDELITVLEKNGLGGFI
                     TPLGQMKAKGEKAVVILP"
     gene            complement(4992..5921)
                     /locus_tag="PLU11_07145"
     CDS             complement(4992..5921)
                     /locus_tag="PLU11_07145"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPN58930.1"
                     /translation="MPIASTLLMVRPASFGYNDETAANNFFQHHPGLDRNTLQQKALA
                     EFDAMVDLLRSHDVELLVLEDSKEPPKPDAVFPNNWISTSPDGKIFIYPMYAPNRRTE
                     KRDELIDQIVREYMVTDVQDWSEFEAEGRFLEGTGSMVIDYDHEMIYAAISERTNLSV
                     LEKFASANGFQAIVFLATDKEGHPIYHTNVVMALGEKFAVLCEEAIEEEWELIAVKQL
                     LESTGHTIIPITRDQLYAFAGNMLEIRNKKGESLIVLSQTAMKALRPDQIQQLQSFAT
                     LVPVAVPTIEQVEGGSVRCMLAEIFLQRKVKEG"
     gene            complement(5931..6293)
                     /locus_tag="PLU11_07150"
     CDS             complement(5931..6293)
                     /locus_tag="PLU11_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58931.1"
                     /translation="MNIKGLLPVFGLVLFACNNGDNVVAGGPCSYKETIHPAKLIKLE
                     TTDSTWYNVWLELEAGIQSKDKKDTLYSRQFNGSLLHIDQIKKDSIAVGNTYKFVVQE
                     ILSGNCTPRVTMFRFEKF"
     gene            complement(6303..6533)
                     /locus_tag="PLU11_07155"
     CDS             complement(6303..6533)
                     /locus_tag="PLU11_07155"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPN58932.1"
                     /translation="MWTHTDNKLYCKFQFSNFSEAFAFMTRVAIEAEKMDHHPLWTNV
                     WNTVEIWLSTHDAGDIVTEKDHLLAEKIDGLI"
     gene            complement(6699..7142)
                     /locus_tag="PLU11_07160"
     CDS             complement(6699..7142)
                     /locus_tag="PLU11_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPN58933.1"
                     /translation="MAEKNKLVLDTKELMEGFFEDTRLLGVMAPIKDYQFCWHLNNTI
                     GLDFRINDIEIRLIKKKRDYFFTVYEYRVPTGTLEHYIYNNQFDGEYLLPEFKHFDFL
                     WLMKGDEVNDDTLQETIQTIRSINSVQLVTELSIEQIRNKENLVF"
     gene            complement(7158..8312)
                     /locus_tag="PLU11_07165"
     CDS             complement(7158..8312)
                     /locus_tag="PLU11_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPN58934.1"
                     /translation="MIVYNTAPAEDKLLFNGKDKSETLTIPVIYIKKEAAAKYLKDPS
                     ATINVKFAVSLSDKKRTGYNVVGYLDNGAATTVVLGAHFDHLGYGEDGNSRNTTTEPA
                     IHNGADDNASGTAALIELSKKLKYSSLKNNNYLFIAFSGEELGLYGSKYFTEHPTIDL
                     KTVNYMINMDMVGRLNDSSKVLTIGGYGTSPTWEKVINNNDFFKLPYKTNVQPPLVIR
                     TDSSGTGPSDHTSFYRKDIPVLFYFTGLHTDYHKPSDDADKINYGGEQLIVKHVLGVV
                     QQLDNQPKLAFLKTREAQTTTSARFSVSMGIMPDYTWSGNGLRSDGVSEGKPAQKAGL
                     KAGDIILQIGEHKITSMESYMQTLGKFKKGDKAKVIYLRGKDTLETTVEF"
BASE COUNT         2367 a         2061 c         1846 g         2118 t
ORIGIN      
        1 tgatttgtct tcaacgacta accccgaatg gggttgaacc attcatagcc ccgggcgcca
       61 acccggggta ggcgatcatt tgaataccct ccaaccccaa cggggttgaa ctattgatga
      121 cccggaaaaa acatctggca cgatttcaaa aacaaacaat attcgacgct tacaaaatgc
      181 cggtgatttg tcttcaacga ctaaccccga agggggttga accattaata gccccgggtg
      241 ccaacccggg gtaggcgata atttgaatgt cctgcaaccc caacggggtt gaactataaa
      301 cttcaccaaa acaacttctg accaatgata taaacctcct gttcaacttg cccttcgaat
      361 tgattatttt tacaaacaac catggcggtc caacgtatcc acatagatga attcctgaaa
      421 ctggcagaaa cacaccctgt attcgatgta cgttcacccg gcgaatacaa ccatgcgcat
      481 atgcccgggg caatttcact tccactattc actgatgaag aacgcaaaca ggtcggcacc
      541 gcttacaaac aaatcagccg cgaacaagcc atcaagatcg gtctcgacta cttcggcccc
      601 aaaatgcgca agatggtgga agaagtagaa gcagtcagca gccggcagtt ggcagacagc
      661 agccggcagg cggcagttgg cagcctgcct actgccaact gcatactggt atattgctgg
      721 cgcggcggca tgcgcagcgg cgcggtctcc tggttgctcg atatgtatgg attcaaagtc
      781 tacacgctta tcggcggata taagaagttc cggaaccatg tactcgatac gtttaaaaaa
      841 ccttaccgct tcaacatcat cggtggatat accggctcgg gtaaaaccga attactcaaa
      901 accctgaaag aaaaagggga gaaggtgatt gacctcgaaa acatcgccaa ccataaaggc
      961 tccgccttcg gtgccatcgg cctgcccccg caacccaccc aggagatgtt tgaaaatctc
     1021 ctcagcaacg aattgtcagc tataagttct aaggagggac tacgaactac taactacgaa
     1081 caactaacta gttgtatctg gctcgaagac gaatcccagc gcatcggcct catcaacatc
     1141 cccggcgacc tctggaaaaa catgcgccaa tcgaaagtct ggttcctgga catccctttt
     1201 gaggaaagat tgaagcatat tatccccgaa tatgggattt tagaacaaga aaagctgatt
     1261 gccgccattg agcgaatcag ccagaaattg ggccatttga acgcaaaaac ggccatttta
     1321 ctgctaaaag agggcaaaat cagcgaaagt tttgacattt tgctccggta ctacgacaag
     1381 ttttatttcc ggtctctgca caatcgggag gggttagaaa cgttattaca tacgataaac
     1441 tgtaaatccg ttagtccaga aaatgccgat ctccttatcc gtgaagccac tgctcaaagc
     1501 cagacacctc tggcttagtt tcctgttcct gcttgccccc ctgttttttt atagccagac
     1561 cctgaccggt ctctgggtag gggctcttca caacgacagc agctctgtac gaaaagacca
     1621 gtcttttgaa atcgctctta ccgaataccg cggaaaggta tttggctatt cccgcagtga
     1681 attcattgta aatgataccc tgtattatat tgttaagcgt gtcaaaggaa ccattgaagg
     1741 ggatatatgt gaagtaaaag acgatgaaat catttcttat aatttcaggg ggaaactgga
     1801 caaggggatt aaagtaacca gtacattccg acgtaaccag actgatagca cctggcagct
     1861 tgacggaagc tggaaaacca atgccactaa aaaatactat tcagttaccg gaaaagtgaa
     1921 cctcgcggag gaaaaagacc tgaccgcatc caaactattt ccccacctcg aagaactgaa
     1981 taaggccgac gaaatggttt tttataaaga aagaaacgaa ccggccccat tcgtaaaagt
     2041 ggcaaaacca gagcgaattc agtcagaata caccgaaaag gccgatattt tgaccaaaaa
     2101 tgaggaaaaa tcgctttcgg tgaaaaaacc ggaattgccc cagccggcgc aacctgtcgt
     2161 gcctgaagtg gcagtacaaa cggttccgga aaccaaaaca gtacccgttt taccagcaga
     2221 tgccttacct ggaaaaaatc cggtggctac caacaacccg gcccaggaaa ctgcagtagt
     2281 gaaaaacgag ccggttctga ccgttgttcc ggataaaaaa atcgcagaac ctaagcccgg
     2341 ggataaactg accgccgaaa ctaacatccc ggtggttgct gcaaaaacag aaacccctcc
     2401 cattagaacg gcagccccgg ctaattcagc taacgaaaca gcaaacacca ataccgctaa
     2461 ccccaaagtc gccgccgtaa agccagtccc ggtaaccaac ccggttgtaa aaacacagcc
     2521 aacagcaccc ccggtaaccg ccccaactgt taaaccaggc ccgacacagg atgaaaaagc
     2581 cgtcgctaaa acaaaaccaa cagcaacagc accggccctg acaccggtca gcaataaaac
     2641 ggttatttcc aatccggttg aaacaaacct gcccaaacca agagtcattg atccaacggc
     2701 cctgctggtt aaaaaatcag tagaagaccc gatcaaaaaa tcaacgatga tcgccggccg
     2761 caaatcagag ttcagccagg aagtctattt caaatccgac agcctgatcc tgacccttta
     2821 tgacaatgga gaaattgatg gagacacggt aagtgtatac ctgaacgggg aagtagtgat
     2881 gcccagccag ggattaaaaa gttcagctat ccgcaaaatc attcatatgg atgacggcct
     2941 cgaaacattt accctggtga tgtttgctga aagcctgggt aaatacccgc ccaataccgg
     3001 tctgctcgtg atccgcgatg gcaatgatgt ttacaatctt cgcttcagtt ccgacttcca
     3061 gaaaagttca gggattgtgt tccggagaaa gcagtaattt aatagcatga agctttccgg
     3121 tcttcttgtt tttttattga ttcatacgat ctgcctgtcc caaaccaccc gtattgatac
     3181 agtctactcc acccaggtaa aagacaagtt tgttattacg gttcgtaaac cggccggcta
     3241 ttccgataca aagaagtatc accacgtata catgaccgat ggttcactcg gtattgcgga
     3301 ttatgtattt ggtaaagaga aatcctgggc cgctaccata cccaacagct gtgtgatcat
     3361 tgccatttct catatcggtg attgggagga aaagagaaga agggatttta ttccatcaga
     3421 cattacgaag aatgtggagg ctaattttgg gaatgcgaat acgttttatc agtttatgaa
     3481 aaaagaactg attccccgaa ttgaaaaaag tatgcccaac aaaaagggcc gtgcttttat
     3541 cggacattcc ttcggcgggt tgttttgctt atatacctta ttacaggagg ataaactgtt
     3601 tgataaacat tttgcgatca gtccatcggt ctgggccaac tataatgaac tggataagat
     3661 cgaagcgcaa tacgcaaaga atcacaaagc cctgaaggcg gatatcacta tttatgtagg
     3721 cggacttgag ttcctgaata aagtgctgta ctccacccgc aacttttata atacagtcag
     3781 tggccggaaa tataccggct gctggatccg gaaaaatgaa atcggaaacg cgaatcactt
     3841 cagtatccgc aaaccggcga ttgaccgcat ttttgaatcg ctgaaatagg aaccggttcc
     3901 attccagcac tgaatgacta actttgctcc ctcaataaat catatgatga cgcaggatcc
     3961 tgtaagactg acccagttct cccatggcgc gggctgtggt tgtaaaattt cgcctaaagt
     4021 actggatgaa attttaaaaa cttctgtagc gttccctgat aatgataaac tattggtggg
     4081 aaatcacagc cgggatgatg cggccgttta tgatctcggt aacgggaccg ccatcatctc
     4141 gaccacggat ttctttatgc cgatcgtgga tgatcccttt gaattcggac ggatcgctgc
     4201 ggccaattcc atcagcgatg tctatgccat gggcggcaaa cccctgatgg ccattgctgt
     4261 tcttggatgg ccggtgaata aattgccggc cgatgtggca cagaaagtag tggaaggtgg
     4321 acgcagcatc tgcctcgagg ccggtattcc actggccggc ggacacagca ttgattctcc
     4381 ggaaccaatc tttggactgg ccgtaaccgg tatacttccc attgaaaacc tgaaacaaaa
     4441 taatactgct aaagaaggcg acctgctctt cctcaccaaa ccactaggag taggtatttt
     4501 atcgaccgca cagaaaagag gattaatcac cgacgacctg ctggccgtga tcatcaaaca
     4561 aatgaccacc ctgaataaaa caggggagga gttgggaaaa attaaaggcg tacacgccat
     4621 gaccgacgtc accggcttcg gcttcctggg tcacctcatt gaaatggccg agggcagcgg
     4681 actcagcgcc gaagtatggt atgataaaat ccctgccatc cccggcgtga aagagtatat
     4741 cgcccagggc gtattccccg atggcaccac ccgcaactgg agcagctatg gcgagaaagt
     4801 aaaatttgaa aaaggcaccc cggtggcgga agcttttaaa ttattaccgg atccgcaaac
     4861 caatggcgga ttattgattg ccattgcgcc ggaaacaaaa gacgaattga ttactgtact
     4921 tgaaaagaat ggactgggtg gattcatcac ccctctcgga cagatgaaag ctaaagggga
     4981 aaaagccgtg gtcatccttc cttaactttt cgctgcagaa agatctccgc caacatacaa
     5041 cgtacactgc cgccttcgac ctgttcaatg gtggggacgg caacgggtac cagtgttgca
     5101 aaactctgta gctgttgtat ctggtccggc cgtaatgctt tcattgccgt ctgactgagc
     5161 acgatcagcg attctccttt tttgtttctg atctcaagca tattgcctgc aaaagcatag
     5221 agctggtcac gggttatggg gataatggta tgacctgtgg attccagtaa ctgtttcacc
     5281 gcaatgagtt cccattcttc ttcgatcgcc tcttcacaaa gcacagcaaa tttctctccc
     5341 agggccatca ccacattggt atgatagata gggtgacctt ctttatccgt ggccagaaat
     5401 acaatggcct gaaatccgtt ggcagaagcg aatttctcca ggaccgataa attagtacgc
     5461 tccgaaattg ccgcatagat catttcatga tcatagtcga tgaccatact ccctgtgcct
     5521 tcgaggaaac gcccttctgc ctcaaactca ctccagtcct gtacatctgt taccatatac
     5581 tcgcggacaa tctgatcaat caattcatcc cttttctccg tacgccggtt gggggcatac
     5641 atgggataaa taaatatttt tccatctgga gaagtactga tccagttatt gggaaatacg
     5701 gcatctggct tgggtggctc cttgctgtct tccagcacca gcagttccac atcgtgactg
     5761 cgcaacaaat caaccattgc atcaaattca gccagggctt tttgttgcag cgtattacgg
     5821 tccagcccgg gatggtgctg gaagaaatta ttggcagccg tttcgtcatt atagccaaac
     5881 gaagccggcc tcaccattaa caaagtagac gcgatgggca aatgatgaaa ttaaaatttt
     5941 tcaaaccgaa acatagtaac gcggggtgta caatttccgc ttagaatttc ctgtaccacg
     6001 aacttataag tattccccac agcaatgctg tcttttttta tctggtctat atgaagcaga
     6061 cttccattaa attgtcttga atacaatgta tccttcttat ccttagattg gataccggct
     6121 tccagttcca gccatacatt gtaccaggtg ctgtccgtgg tttcgagttt aatcagtttc
     6181 gccggatgaa tggtttcttt atagctgcag ggcccgccag caaccacatt gtccccgtta
     6241 ttacaggcaa aaagtaccaa accgaatacc ggcaacagcc cttttatatt catagtatta
     6301 aattaaatca atccatcaat tttttcggcg agcaagtggt ctttttccgt aacgatatcc
     6361 cccgcatcat gcgtactcag ccagatctct acggtgttcc acacattggt ccatagcgga
     6421 tgatgatcca tcttctccgc ctcaatcgcc actcttgtca taaaagcaaa agcttcgctg
     6481 aagttgctga actggaattt acagtagagt ttgttgtcag tatgagtcca catagtatga
     6541 agcgttttga aaaatgagta atcaataatg agtaaaggtg gaaaagaata aacggaaaga
     6601 acatttccca atgcgggaag ttcagaaaga tagttatgtg cgatcagcta ctcattactg
     6661 attactcatt actcatccta acttatgccg gttcctcatt aaaacacgag attttcttta
     6721 ttcctgatct gctcaatcga caactccgtc accagctgca cactattaat actccggatc
     6781 gtctggatcg tttcctgcaa cgtgtcatca tttacttcat cgcctttcat cagccagaga
     6841 aaatcgaagt gcttgaattc gggcaagagg tattcgccgt cgaactggtt gttataaata
     6901 tagtgttcca gggttccggt gggaacccgg tactcgtaca cggtaaaaaa ataatcccgt
     6961 ttctttttga tcagcctgat ctcaatatcg ttgatccgga agtcgaggcc aatcgtatta
     7021 ttcaggtgcc agcaaaactg gtagtccttg atcggggcca tcacgcccag caaacgggtg
     7081 tcttcaaaaa agccctccat gagctccttg gtatccaaca ccagtttgtt tttttcggcc
     7141 atactgtaat ttacggatta gaattcaacc gtggtctcca gggtgtcttt cccgcggagg
     7201 taaatgactt tcgctttatc tcctttctta aacttaccca gggtctgcat ataactttcc
     7261 attgaggtga tcttatgttc cccgatctgg aggataatat cccctgcttt cagtccggct
     7321 ttctgcgccg gcttaccctc acttacccca tcagaacgca acccattacc ggaccatgta
     7381 taatcaggca tgatgcccat ggatacactg aaacgggctg aagtagtggt ttgcgcttcg
     7441 cgggttttga gaaaagcgag tttaggttgg ttgtccagct gctgcactac acccaacaca
     7501 tgctttacta ttaattgctc cccgccgtag ttgatcttat ccgcatcatc cgacggttta
     7561 tggtaatcgg tatgcaggcc ggtaaaataa aacaataccg gaatgtcctt ccgatagaag
     7621 gaagtgtgat cactgggacc ggttccgctg ctgtcggtgc ggatcaccaa cggcggctgt
     7681 acattagtct tatagggcaa tttgaaaaaa tcattgttgt tgatcacttt ctcccaggtc
     7741 ggagaagtgc cataaccacc aatggtcagt actttgctgc tgtcgttcaa ccggcccacc
     7801 atatccatat tgatcatata gtttacggtc ttcaggtcaa tggtcggatg ttcggtgaaa
     7861 tacttggatc cgtaaagacc gagttcctcc ccggaaaaag caataaagag atagttatta
     7921 ttctttaaag aagaatattt taattttttg gacaattcga taagtgcggc agttccgctg
     7981 gcattatcat ccgcgccatt atgaattgca ggttcagtcg tagtgttccg ggagtttcca
     8041 tcttctccat aacccaggtg atcgaagtgc gcacccaata ccaccgtggt ggccgcgcca
     8101 ttgtccaggt aaccgaccac attatagccg gttctttttt tatcactgag tgaaaccgcg
     8161 aatttcacat tgatcgtggc ggaagggtct ttcaggtatt ttgcagcggc ttccttttta
     8221 atataaataa ccggaatggt cagggtttca gatttatcct ttccgttaaa aaggagttta
     8281 tcctctgcgg gagctgtatt gtaaacaatc accgctgtgg cacccttttc ctttgctttt
     8341 ttggaattgg tatatatatc tcattcaaat ctctttttct cccgcctgca tt
//
LOCUS       DAOPMW010000116         8353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9473_length_8353_cov_7.523500, whole genome shotgun sequence.
ACCESSION   DAOPMW010000116 DAOPMW010000000
VERSION     DAOPMW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9473_length_8353_cov_7.523500"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2839)
                     /locus_tag="PLU11_07170"
     CDS             complement(53..2839)
                     /locus_tag="PLU11_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN58935.1"
                     /translation="MLTLLKSMQTACRGITITLLLLLSLVATAQAQREVKGKVTDDKG
                     QPAASITVTEKGTRNGVTTGADGSFTIKVKDGSTLVFTGISFETKELKTGSESYYDVT
                     ITQTASTLTDVVVVGYGKSSKKNLSSSVTTLKPGDLNRGAIADVGQLLQGKVAGLNIT
                     ASGDPNRPAAVILRGASTVNSPGAPFYVIDGVPGADIATVAPDDIVSVDVLKDAAATA
                     IYGNRAASGVIIITTRRGKKGSAVATYNGYVGVETVSSKLDLMNAAQHRAYLTANSAA
                     YSPIDDLGANTDWMEAIQRSQAISHNHNLSLSGGGEKSTYSASLNYFDKEGILNRSKL
                     RRIIGRLSLEQYALNDKVKFGLTISNASSNANYVPLQNIVLLQAAKHLPVSPVKNADG
                     TYFENLNTTGYFNPVAIVDNAKDDTKYNTLTGNFTTEVKLPFDLQFNLNLSYQNTTSL
                     HGEYYGSYYGKYPTSNFYNNPDPGIGIAHTLIGNLFGVNGSAFRNSFQNTFKAAESYL
                     TWNRKFGNHSINAVVGYSWQENTYGEGLSVSSTNFPSDNLGYTNLVLGNPYAIPSYRI
                     NLGNDLTYGQTRLISDFGRINYNYNDRYLLQASIRRDGSSVFGENNAWGYFPSVGAGW
                     RISNESFMQNQKIFNDLKLRFSYGVTGNSGGIGAYTSKLIYGITGTYYNNGIQENAYG
                     PIQGSNPDLQWEEVATTNVGLDFTILKNKISGSLDWYSKKTTDMLFNYRVSASLVPGG
                     SIWANGGSISNQGVELSLNATPVSNKMLTWTTTFNIATNKNEITSLAGPYANGDSIRY
                     SDPEGPGQTGATLQLLKVGKPIGQFFSLRYAGKDQNGVSTFFKRDGTTTTSPAIGTDY
                     FYVGDAQPKFLMGWSNSVRYGNWDLNVFLRGVFGNKIFNATRADLSYVVAAAQNNILN
                     SASGDKINDNRNSY"
     gene            complement(2876..3751)
                     /locus_tag="PLU11_07175"
     CDS             complement(2876..3751)
                     /locus_tag="PLU11_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HPN58936.1"
                     /translation="MSNARFESVPLTNLDDWEDDLLRRYPDPEAIATAKATGAYRNYE
                     EPARDTVKEFYRLNHQYQTYAFVQEKRSQFLKFDKKEMTVWDAFDFLNQLVDDSDPDT
                     DLDQLQHLLQTSEAIRRDGHPDWMVLTGLMHDMGKVLCLFGEPQWAVVGDTFPVGCAF
                     SDKIIYPEYFSANPDHADERYNTKLGVYTENCGLRNVDMSWGHDEYVYQMLKDYIPEP
                     GLYMLRYHSFYSWHREGGYEYLLDDHDREMLKWVKLFNPYDLYSKSPEPPDWLKLRPY
                     YEELVAKYLPAKLKF"
     gene            4052..5062
                     /locus_tag="PLU11_07180"
     CDS             4052..5062
                     /locus_tag="PLU11_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN58937.1"
                     /translation="MSKPTIKVLAKELGLAVSTVSKALRDSHEISQETKDKVLKLAAQ
                     LNYIPNPYASSLRRQKSRTIAVVIPEVADSFFSIAINGIEEVAREKGYHVLIYLTHES
                     LERETEILNEFKSGRVDGLLISVTRDTCTGTHITEAIEAGLPVVFFDRMCEDIKAARV
                     LTDDYESSYQATRLLIERGCKKPAYLSISPNLSINQKREAGYLAAVNEAGIETGQAMI
                     INCDGTEDKITEQILILLRQPNRPDGIIASVEKLVPPVYFACKELGINIPEEIRLIGF
                     TNLTASPILNPSLSTVTQPAFEIGKTAAALLFKGIEKTSFDLSREKLIIPSQLQLRDS
                     TL"
     gene            5116..5772
                     /locus_tag="PLU11_07185"
     CDS             5116..5772
                     /locus_tag="PLU11_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_116849107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPN58938.1"
                     /translation="MTKAFLFDLNGTMVDDMHYHIKAWHRISNELGASLSMEAVKAEC
                     YGKNAEVMERIFPGRFSPDEKNKISIEKEIQYRAEFKPFLKLIDGLDLFLEKAKANGW
                     KMAIGSAAIMSNVDFVLDGLQIRHYFDAVISADEVTNSKPDPETFLRCAAALGADPAR
                     CIVWEDAPKGVESALNAGMKSVVLTTMHEAHEFSQYPNILLFTHDYTHPGLQQILIKN
                     "
     gene            5843..7477
                     /locus_tag="PLU11_07190"
     CDS             5843..7477
                     /locus_tag="PLU11_07190"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPN58939.1"
                     /translation="MEPATYVIGADFGTDSVRSVIINSRNGQEITSSVFYYPRWKQHL
                     YCNPSSQQYRQHPLDYLEGIETSVRHCVEQAGPEVAKRIKAISVDTTGSTPVAVDKTG
                     TPLCFLPGLENNPNALFVLWKDHTSTGEAEEINKHAAKYPVNYLQFVGGIYSSEWFWA
                     KLLHILREDEAIRNNIYSFVEHCDWIPYVLTGGTDAAQLKRGVCSAGHKALYAAEFGG
                     LPPDTFFSELDPLLKGFTERLFTRTYTSAEKAGTISPDWAIKLGLPKDVVVGIGAFDC
                     HMGAVGGQIEPYYLSKVMGTSTCDILVAPKADMTDKLVRGICGQVDGSVIPGMIGLEA
                     GQSAFGDAYAWFKNLLSWPLQEIEDETLRNNITDNLINRLSTEAAKIPLNEKDELALD
                     WLNGRRTPDADQLVKGAFTGLDLSTDAPRMFKALVEATCFGAKAITDRFVEEGIPVKG
                     LIALGGVARKSPFVMQTMADVMNMPIRIHSSEQTCAAGAAMFAATAAGIYATVEDAMK
                     AMGQGFDKEYKPIRKNVALYKKRFKRYKKLGVFVAR"
     gene            7499..8197
                     /locus_tag="PLU11_07195"
     CDS             7499..8197
                     /locus_tag="PLU11_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HPN58940.1"
                     /translation="MSKYTAIQEEAYAANMQLPQLGLVLFTFGNVSAADHSLGVFAIK
                     PSGVAYEELSPDKMVIVDFDGITVEGSLRPSSDTKTHAVLYKHWSKIGGVVHTHSTYA
                     TAWAQAQRDIPIFGTTHADYNTVDIPCAPPMSDEMIKGNYEHETGFQILNCFADKKLN
                     YEEVEMVLVGNHAPFTWGKNAAKAVHNSAVLEFIAKTSLLTEQINPNAPRLKDALKQK
                     HYERKHGPDSYYGQ"
BASE COUNT         2245 a         1977 c         1967 g         2164 t
ORIGIN      
        1 tcagtcgcgc catgcagttc tttttcacca gcacgcctta taacagggtg gactaataag
       61 agttccggtt atcgttgatc ttatctccgc tggcactgtt caggatatta ttctgagcag
      121 ccgccactac ataggaaaga tcggcgcgtg tagcattaaa gatcttattg ccaaatacgc
      181 cgcggaggaa tacattcaga tcccagttgc cataacggac actgttactc cagcccatca
      241 ggaatttagg ttgtgcatca cctacgtaga agtaatccgt tccgatagcc ggactggtag
      301 tagtagtacc atctctttta aagaaggtag atacgccatt ctgatctttg ccggcataac
      361 gcagggagaa gaactggccg atgggtttgc ccactttcag cagttgcagc gtagcgcctg
      421 tctgaccggg gccttccgga tcggaataac ggatcgaatc accattggca taaggacccg
      481 ccaggctggt gatctcattt ttatttgtag cgatattaaa ggtcgtggtc caggtcagca
      541 tcttgttgct gacaggtgtt gcattcagac tcagctccac tccttggttg ctgatgcttc
      601 cgccattggc ccagatactt ccaccgggta caagcgaagc agacacgcgg taattaaaca
      661 gcatatccgt ggttttctta ctgtaccaat cgagtgaacc actgatttta tttttcagga
      721 tggtaaagtc aagtcccaca ttcgttgtgg ccacttcttc ccattgcaga tcggggttag
      781 aaccctggat cggaccatag gcattctcct gaataccatt gttgtaatac gttccggtaa
      841 taccatagat cagtttgctg gtataggcac cgataccacc ggagttaccg gttactccat
      901 aactgaagcg aagtttcagg tcattgaaga ttttctgatt ctgcatgaag gattcattgc
      961 tgattctcca gccggcaccc acggagggga aataacccca ggcattgttt tctccgaata
     1021 cagagctacc atcccgacgg attgacgcct gcagcaggta ccggtcattg tagttataat
     1081 tgatacgacc aaaatctgaa atcagccggg tctgaccata ggtcaggtca ttgccgagat
     1141 tgatgcggta gctgggaatg gcataaggat tacccagtac caggttggta tatccgaggt
     1201 tgtcggaagg aaaattcgta ctcgataccg acaggccttc accataggtg ttttcctgcc
     1261 aggagtaacc caccacggca ttgatgctat gattcccaaa tttacgattc caggtcaggt
     1321 atgattcggc tgctttaaat gtattctgaa atgaattacg gaaagcagaa ccattcacgc
     1381 caaataaatt accaatcagg gtatgggcaa taccgatacc gggatcagga ttattgtaga
     1441 agttggatgt aggatattta ccataataac taccataata ctcaccatgg agtgaggtgg
     1501 tattctggta tgaaaggttc aggttaaact gcaggtcaaa aggcaactta acttcggtag
     1561 tgaaattacc cgtcagtgta ttgtacttgg tatcatcctt tgcattgtcc acaatcgcta
     1621 ccgggttgaa ataaccggtt gtattgaggt tttcaaagta cgtgccgtcg gcattcttca
     1681 ccggggaaac aggtaagtgt ttggcagcct gtaagagtac aatattctgc agcggtacat
     1741 aattggcatt gctgctggcg ttggagatcg tcagtccgaa tttcacttta tcattcaggg
     1801 catattgctc caggctcagg cgtccgatga tccggcgcag tttactgcgg ttcaggatac
     1861 cttctttatc aaaatagttg agactcgcac tatacgtact cttttcgcca ccgccgctga
     1921 gtgaaaggtt atgattatgg gagatggcct gtgaacgctg aatggcttcc atccagtcgg
     1981 tattggcacc cagatcatcg atgggtgaat aggcagcact attggcggtg agataagcac
     2041 ggtgctgagc agcattcatc aggtcgagtt tgctgctcac tgtttccaca cctacataac
     2101 cattataagt ggcaacagcg gatccttttt taccacgacg tgtcgtgatg atgattaccc
     2161 cgctggcagc ccggttaccg tagatcgctg tggcagcagc gtctttcagt acgtcaacgg
     2221 acacgatatc atcgggagct acggtggcaa tatccgcacc gggtactcca tcaattacat
     2281 agaagggggc acccggactg tttacggttg atgcaccccg caggattaca gcagccggac
     2341 ggttagggtc accactggcg gtaatgttca gtcccgccac tttcccctgg agcaactgac
     2401 ctacgtctgc aatggcgcca cggttcaggt caccgggttt gagggttgtg actgaactgg
     2461 agaggttctt tttactgctc ttaccataac ctaccactac cacatccgtc agggtgctgg
     2521 cagtttgagt aatggtcacg tcgtagtaag attcacttcc cgttttcagt tctttggttt
     2581 caaatgaaat gccggtaaac accagggtgc ttccgtcttt tactttaatg gtgaaagaac
     2641 catccgcacc ggtagtcacc ccgttacgag tacctttttc ggtcaccgta atggatgcgg
     2701 ccggctggcc cttatcatcc gtcacttttc cttttacttc ccgttgggcc tgagcagtgg
     2761 ctaccaggga caagagcagc aacaacgtaa tcgttatgcc gcggcatgca gtttgcatcg
     2821 attttagcag agttaacatg gcagatgttt atcgttttag gttaataatg aacagttaga
     2881 attttaattt ggctggcagg tatttagcaa ccagttcttc ataataaggc cgcagtttta
     2941 accagtccgg cggctccgga cttttagaat agagatcgta aggattaaag agtttgaccc
     3001 atttcagcat ttcgcggtca tggtcatcca ggaggtattc ataccctcct tcacgatgcc
     3061 aggaatagaa agagtgataa cggagcatgt ataaaccggg ctcggggata taatccttaa
     3121 gcatctggta tacatattca tcgtgtcccc aggacatatc tacgttccgt aaaccacagt
     3181 tctccgtata tacgccgagt tttgtattgt agcgttcatc cgcatgatcc ggattggctg
     3241 aaaaatattc cggataaatg attttgtcag aaaatgcaca acctaccggg aaggtatccc
     3301 ctacgacagc ccactgtggt tcaccgaaca ggcataagac tttccccata tcatgcatca
     3361 gaccggtcag taccatccag tcgggatgac catccctgcg gatggcttcg gacgtctgca
     3421 acaggtgttg aagctggtcc aggtcagtat ccggatcaga atcatccacc aattgattca
     3481 ggaaatcgaa agcgtcccat actgtcattt cctttttgtc aaacttcaga aactggcttc
     3541 ttttttcctg tacaaatgca tatgtctggt actggtgatt cagccggtaa aattctttca
     3601 ccgtatcacg ggccggctct tcataattgc gataggctcc ggtggctttc gcggtggcaa
     3661 tggcctccgg atcaggatag cgccgtaaga gatcatcctc ccagtcatcg agatttgtca
     3721 gtggaacaga ttcaaacctg gcattactca tagctgcttt tttaaatagt tgtgctgatt
     3781 aaatgatagt tgcagaagac cggcgattgc tgaaagctga ggtgcccgct tgaagaggtg
     3841 gctgattctg cgggtacaga acagcattta gcagagagat gcggcaagca tttcatacta
     3901 cagacaaatc gttggtttct tgttataaat gttaaaaaga cactttttat ctgaaaccta
     3961 ttgcatctgt gtctgatttt tttgcgaaac cgttttcgcc aattaaccta tatttagggc
     4021 ggacagcaga ctgattcaca ccctttaaca tatgagtaaa cccacgatta aagtactggc
     4081 gaaagaactg ggactggcgg tttccactgt atcgaaagca cttcgggaca gtcatgagat
     4141 cagccaggag acgaaggata aggtattgaa actggcggcg caactcaatt atataccgaa
     4201 tccttatgcc agcagtctgc gccggcaaaa aagccggacg atagcagtgg tcatccctga
     4261 agtggcggat agtttttttt ccattgccat caatgggatt gaagaagtag ccagggagaa
     4321 aggctaccat gtgcttattt atcttacaca tgaaagtctg gaacgggaga ccgagatcct
     4381 gaatgaattt aaaagcggcc gggttgacgg gctattaatt tctgttaccc gtgatacctg
     4441 caccggtacg catatcaccg aagccattga agccggatta cccgtggtct tttttgaccg
     4501 gatgtgtgaa gacatcaaag cggcccgtgt gcttacggat gattatgaaa gcagttacca
     4561 ggccacccgc ctgttgattg aacggggttg taaaaaacca gcttatcttt ctatttcccc
     4621 gaatctgtcc atcaatcaaa aaagggaagc cggttatctc gccgccgtga acgaagccgg
     4681 tattgaaacc ggtcaggcca tgattatcaa ttgtgacgga accgaagata aaatcaccga
     4741 acaaatactt atcctgctcc ggcagccaaa cagaccggat ggcatcattg cctctgtgga
     4801 aaaactggtg cctcccgttt attttgcctg taaagaactg ggtataaaca ttcctgaaga
     4861 gatccggctg atcggcttta cgaacctgac tgcttccccg atcctgaacc cttcactcag
     4921 tacggtaacc cagccggcat ttgaaattgg taaaacagct gcagcccttc ttttcaaagg
     4981 cattgaaaaa accagtttcg acctgagccg ggaaaaactg atcatcccct ctcaattaca
     5041 attgcgcgac tctactttgt aaatgacttt tgtttattca tccattgcgg gtatttttga
     5101 ccccactaca actacatgac aaaagcattc ctctttgatc tcaatggtac catggtggat
     5161 gatatgcatt atcatattaa agcctggcac cggatatcca atgaactggg cgcctcactc
     5221 agtatggaag ccgtaaaagc ggaatgctac ggaaagaacg cagaagttat ggaacggatt
     5281 ttccccggcc gtttcagccc ggatgaaaaa aataagatca gtatcgaaaa agaaatccag
     5341 taccgcgcgg aattcaaacc ttttcttaaa ctgattgatg gcctggacct gttccttgaa
     5401 aaagccaaag ccaatggctg gaagatggcc atcggttctg ccgctatcat gtccaatgtg
     5461 gattttgtac tggatggttt acagatccgg cattattttg atgcggtgat cagtgcagac
     5521 gaagtgacaa acagtaaacc ggatccggaa acttttttac gatgtgctgc tgccctcggg
     5581 gcagatcctg cacgttgtat cgtatgggaa gatgccccca aaggggtgga atccgccctt
     5641 aatgccggta tgaaatcagt cgtgctgacc accatgcacg aagcccatga attcagccaa
     5701 taccctaata ttctgctgtt tacacatgat tacacccacc ccggtcttca acaaattctg
     5761 attaaaaatt aataaatgta ttttttacac ttattaatta ttttcctata ttcggtttta
     5821 attcagcttg tcaacaccgc atatggaacc agcaacttat gtgatcggcg ccgactttgg
     5881 aacagactca gtccgctccg taattatcaa cagccgtaac ggtcaggaaa tcacttcctc
     5941 cgtattttat tacccccgct ggaaacagca tttatattgt aacccgtcca gtcagcaata
     6001 ccggcagcat ccgctcgatt acctcgaagg gattgaaaca tcggtacgtc attgtgtgga
     6061 acaggcagga ccggaagtag ctaaacgcat taaagcaatt tcagtagata ccacggggag
     6121 tacacccgtt gccgtggata aaaccggtac cccgctttgc ttcttaccgg ggctggaaaa
     6181 caatcccaat gccctgtttg tactgtggaa agatcatacg tccaccggtg aagcagagga
     6241 aataaataaa catgcagcaa aatatccggt gaactatctg caattcgtgg gcggcattta
     6301 cagcagtgaa tggttctggg ccaaactgct gcacatcctg cgggaagatg aagcgatccg
     6361 gaataatata tacagttttg tggagcattg cgactggatt ccttatgtgc tcaccggagg
     6421 tacggatgca gctcaattaa aaagaggcgt ctgcagtgcc ggtcataaag cactttatgc
     6481 tgccgaattc ggaggtctgc cgcctgatac ttttttcagt gaactggatc ctttattaaa
     6541 aggatttact gaacgccttt ttacccgcac atatacctcg gcagaaaaag ccggtaccat
     6601 atcccctgac tgggccatta aactcggtct tcccaaagat gtggtggttg gcatcggtgc
     6661 ctttgattgt catatggggg cagtcggcgg acaaatcgaa ccttattacc tgagtaaagt
     6721 catgggtaca tccacctgtg atatactggt agcacccaaa gcagatatga ccgataaatt
     6781 agtccggggc atctgcggtc aggtagacgg ctctgttatt cccggaatga tcggtctcga
     6841 agccggacaa tccgcttttg gtgatgccta tgcctggttt aaaaatttac tcagctggcc
     6901 tttacaggaa attgaagacg aaacactccg gaacaacatc acagataacc tgatcaaccg
     6961 gctttccact gaagcagcaa aaattccgct gaacgaaaaa gatgaactgg ccctcgactg
     7021 gctcaatgga cgccgcacgc ctgatgctga tcagttggta aaaggtgcat tcaccgggct
     7081 tgatctgagt accgatgccc cgcgtatgtt caaagcactg gtggaagcca cctgcttcgg
     7141 cgccaaggcc attactgacc gttttgttga agaaggcatc ccggtaaaag gactgattgc
     7201 tcttggcggg gtagcccgta aatcgccgtt tgtaatgcag accatggctg atgtgatgaa
     7261 tatgccgatc cgcatacaca gcagtgagca aacctgtgcc gccggtgctg ctatgtttgc
     7321 cgcaacggct gccggcatat atgcaactgt agaagacgcc atgaaagcca tgggacaggg
     7381 ttttgataag gagtataaac ccattcgtaa aaatgtggcg ctctataaaa aaagatttaa
     7441 acgatataaa aaactgggcg tattcgtggc ccgttaagtt taccgacaac tattcgatat
     7501 gagtaaatac acagctattc aggaagaagc ctatgccgcc aatatgcagt tgccgcaact
     7561 gggactggtc ctgtttacgt ttggcaatgt cagtgctgcc gatcattcac tgggcgtatt
     7621 cgccatcaaa ccaagcggcg tggcatacga agaactgagt ccggataaaa tggtgatcgt
     7681 ggactttgac ggtattactg ttgaaggctc cctccgccct tcctcagata ctaaaacaca
     7741 tgctgtatta tacaaacact ggagtaagat tggcggtgtg gtacatacgc acagtactta
     7801 tgccaccgcc tgggcacaag cgcaacgcga tattcccatc ttcggcacca cccatgcgga
     7861 ttacaatacc gtggatattc cctgtgcccc accgatgagc gatgaaatga tcaaaggaaa
     7921 ctatgaacat gagaccggct ttcagatcct gaactgcttt gcggataaaa agcttaatta
     7981 cgaagaagtt gaaatggtac tcgtgggcaa ccatgccccc tttacctggg gaaagaatgc
     8041 cgccaaagcc gttcataaca gtgcggtact ggaattcatt gctaaaacgt ccctgttgac
     8101 tgaacaaatc aatcccaatg cgccaagatt aaaagatgcg ctcaaacaaa aacattacga
     8161 acgcaaacac ggaccggaca gttactacgg acaataatta ctattcaata aaaagctata
     8221 tgaaaaaaat cagcacttta ctttctgcag ctcttgccct gcaactggct gcctgtaata
     8281 acagcagtga aacggctaaa gtcgaagaaa ctgcaacact gaccgctaaa gccggcattc
     8341 aggaaaaacc att
//
LOCUS       DAOPMW010000117         8343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9485_length_8343_cov_7.624276, whole genome shotgun sequence.
ACCESSION   DAOPMW010000117 DAOPMW010000000
VERSION     DAOPMW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9485_length_8343_cov_7.624276"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2734)
                     /locus_tag="PLU11_07200"
     CDS             complement(<1..2734)
                     /locus_tag="PLU11_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN58941.1"
                     /translation="MSKLRLLLLGFVLFSAQILSAQNREVTGKVTDAGGSPLSNVSVT
                     AKGSRNGTATANDGTFTLTVDANTKSLVVSAIGFETREIAITGAPLTISLTAGDSRSM
                     DEVIVVGYGTKIKKDLTGNIARIKGSEVANTPVANFTQAIQGRAAGVFVESQSGKVGE
                     GIKVRIRGAGSISASNTPLYVVDGIPINGDGNSGNNLADINFNDIETFDILKDASAAA
                     IYGSRAANGVVLITTKKGKIGKTQLNVNFQYGTNKPTGYRGFLDAKNYVDLLREAAIN
                     SDVIEGVDPLDPAQYGGSWLEFAENRLTRYSGFSNWRNLETNTNWEKLAFNEEAKTRM
                     VDISATGGTDKTRFFLSGGFNDQDGILFLNSFQRLSTRFNLDHDASDRLKLGFNISLA
                     RTIANRVADDNEFYTPMQIVALAPITPVRDQNGALYDRPTTTYYNPLIETEDSRYFST
                     TFRNIGGLYLNYKLSPGLIFKTEFSADIQNQSDDRFYGNRTIIGQSTNGYGEASWYRA
                     VTYNTNNYLSYNKIFKQIHDVDATAGMSYQDYRFDNVFVSGEDFPVNALKRLTSAGRI
                     TGGSTNGNSRAILSYFARGNYKLNNKYLVSLSGRIDGSSAFGQDNKYGFFPAASAGWI
                     LSQENFLNSVKWLSFLKLRASYGLTGNDAGFGAFSHLGLWGASKYNNQSGLVPTQLAN
                     PDLKWEKSAQTDIGIDFGLFGNRLTGEIDYYVRNTRDLIYNVPVPGTSGFTTQTVNIG
                     SMENKGFEFVLNSVNVNSRSFKWNTNLNLSKNSNKVTKLDGDQTLIPGNDGRYMNSLI
                     VGEGIGVFYGPKFAGADPANGDALYFLPDGKNTTNDYNDAGDFVVGNPNPDWIGGLNN
                     TFSYKGIEMSVLFQGVFGNQVMNGAGGFMSASFDWFDNQTVDQLKRWQNPGDDTD"
     gene            complement(2857..3552)
                     /locus_tag="PLU11_07205"
     CDS             complement(2857..3552)
                     /locus_tag="PLU11_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPN58942.1"
                     /translation="MTSEIFIVSLVFLGLSFLVSAVLKSKFKKYSKIGLASGLSGREI
                     AEKMLRENAIYDVKVISVDGFLSDHYNPVNRTVNLSPEVFNGTSISAAAVAAHECGHA
                     VQHATQYGPLMFRSKMVPAVQWSSMLVNWILLAGMVVLASTKNPTILLVGIIIMAVTV
                     LFTLVTLPVEFDASKRALAWLEQTNITNSVEFPKAKDALKWAATTYVVAALAAVVQLI
                     QYIMIYMGSRDRS"
     gene            4035..5408
                     /gene="radA"
                     /locus_tag="PLU11_07210"
     CDS             4035..5408
                     /gene="radA"
                     /locus_tag="PLU11_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPN58943.1"
                     /translation="MSKAKSTFFCQQCGHESVKWAGQCPSCGQWNTFVEELVQKDNGK
                     NNPGWKDYNSEKRTAKTVSLNEIKNTEERRLPTADAELNRVLGGGIVPGSLVLVAGEP
                     GIGKSTLFLQNGLWLKEIVVLYISGEESEQQLKMRADRLGLKNDQFYLLTETSTQIIF
                     QEIKKLKPQLVIVDSIQTLHTTHIESSPGSVSQIRECTAEFQRFAKETNTPVFLIGHI
                     TKDGSIAGPKILEHMVDTVLQFEGDRHYAYRILRTLKNRFGSTAELGIYEMTDSGMRG
                     VLNPSEILITQKEDQLSGIAIAATIEGMRPLLIEVQALVTQSVYGTPQRTVSGFDLRR
                     LQLLLAVLEKRGGFHFGMKDVFLNIAGGIKVEDPSIDLAVLCALLSSYEDVTLPQHIC
                     FAGEVGLSGEIRAVNRIEQRIAEAEKLGFEKILVSKYNQKGLAKQKFNIEVVTMGRVE
                     EVYRYLF"
     gene            5445..6143
                     /locus_tag="PLU11_07215"
     CDS             5445..6143
                     /locus_tag="PLU11_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPN58944.1"
                     /translation="MLLLKEMKDSLLHFLFPHTCCGCGTDILPEDSTLCLRCLHALPE
                     TCFEWHPSNPAEKMFWGRLPLQSASATYYFTKESLVQHLMHQLKYKGNKELGVQLGML
                     MGEALKKSGRFPADALIPLPLFPAKEKRRGYNQATILCEGISAVLHIPVWDQVVKRPE
                     HTETQTKKGRVERWKNMEGKFIVQDTPLLQNKRLLLVDDVVTTGATLESCGAELLKIP
                     GVQLSIATLCIAAR"
     gene            6174..7559
                     /locus_tag="PLU11_07220"
     CDS             6174..7559
                     /locus_tag="PLU11_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPN58945.1"
                     /translation="MKLPGLLVISLLLLLHSCRKDSFITSPEARIGLSIDTLKFDTVF
                     VSAGSTFRTLIIKNENDQQLRLSSLKLMGGNASVFKMNVDGTPGHQFSNLDIAANDSL
                     FVFIQVNVNPNAANLPFVIRDSIQIEFNGNNKKVQLEAWGRNAHFYRDRVISNDESWN
                     NDLPYVILGSLVVPDGKTLTINKGCRIYVHADAPLIVDGTLIVNGEKDTSDRVYFQGD
                     RLDDPYKDYPASWPGLFFLSNAKDNVLNYAVIKNAYQAIGMQGPALNSNPKLILNETV
                     IDNAYDAGIIAINSSLRARNCLITNCGKNLYLVQGGNYQFTHCTVVTYANRFIEHKEP
                     VLFLSNTANSVTNSLDAIFRNCIFWGENGVVTDEVAVEKSGTGTFNVNFDYTLWKVQA
                     PPANSTITQAINNQTPLFDSINTSDNYYDFRLKTGSPALNKGVNAGIFTDLDGKIRPV
                     GLPDLGSFEKQ"
     gene            7640..8158
                     /locus_tag="PLU11_07225"
     CDS             7640..8158
                     /locus_tag="PLU11_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPN58946.1"
                     /translation="MKALVITLIMSTFLTAGSIYDFKVTGLDGKDIDLSAYKGKKILI
                     VNTASKCGLTPQFEGLEALYKKYKGKLVILGFPANNFAGQEPKSNEEIGEFCKQNYGV
                     TFPMAEKISVKGDDTHALYKFLKEQAAAKGFADPVTWNFGKFLINEKGELIATFSPKT
                     TPMSDEILKYLN"
BASE COUNT         2358 a         1913 c         1827 g         2245 t
ORIGIN      
        1 catcggtatc atcaccggga ttctgccagc gtttcagctg atcaaccgtc tggttgtcga
       61 accagtcgaa gctggctgac ataaatccgc cggccccatt catcacctgg ttgccaaata
      121 cgccctggaa aagcacagac atttcaatgc ctttataact gaaggtattg ttcagaccac
      181 caatccagtc ggggttaggg ttacctacca caaaatctcc cgcatcatta tagtcattgg
      241 tggtgttctt gccatccggc aggaagtaca gggcatctcc atttgcagga tctgctccgg
      301 caaacttagg tccgtaaaac acaccaattc cttcgccgac aatcagggag ttcatataac
      361 ggccatcatt acccggaatc agggtttgat caccgtccag cttggttact ttattggaat
      421 ttttcgaaag attgaggttt gtattccatt tgaaactacg ggagtttaca ttcacgctat
      481 tcagcacaaa ttcaaaccct ttattctcca tggaaccgat attcactgtt tgcgtggtaa
      541 aaccggaagt gcccggaacg ggtacattat aaatcaggtc gcgggtgttg cggacatagt
      601 aatcaatctc ccccgtcagg cggttgccaa ataacccaaa gtcgataccg atatctgtct
      661 gggctgattt ttcccatttc aaatccgggt tggccagctg agtgggcacc agtccggatt
      721 gattattgta ttttgaagca ccccacaaac caaggtggct aaaagcacca aagcctgcat
      781 cattacctgt cagcccgtaa ctggcgcgca gtttcaggaa gctgagccat tttacactat
      841 tcaggaagtt ttcctgggaa agaatccagc cggcagaagc agccgggaag aaaccatact
      901 tgttatcctg gccaaatgcc gaagatccgt caatacgacc actcagagaa accagatatt
      961 tgttattcaa cttataattc cctctggcaa aataagaaag aattgcccgt gaattcccat
     1021 tcgttgatcc tccggtaata cgtccggcac tggtcagtct cttaagcgca ttcacaggaa
     1081 aatcttctcc tgaaacaaat acattgtcaa acctgtagtc ctggtatgac atacctgccg
     1141 tggcatcaac atcatgaatt tgtttaaaga ttttattata acttaaataa ttgttggtat
     1201 tataagttac tgcgcgatac cagctggctt caccataacc attggttgac tgaccgataa
     1261 tcgtacggtt tccataaaaa cgatcatcgc tttgattttg gatatcagca gaaaactcgg
     1321 ttttgaatat aagtcctggt gacagtttgt aattcaaata caagcctccg atatttctga
     1381 aggtcgtaga gaaatacctt gaatcttcgg tttcaataag cgggttataa taagtagtgg
     1441 ttggacgatc atacaatgct ccgttttgat cgcgaacagg tgttataggc gccagtgcta
     1501 ctatttgcat cggggtatag aattcattat catctgccac acggttagca attgttctgg
     1561 ctaaactgat attgaagcca agtttgagcc ggtcagatgc atcatgatcc agattaaacc
     1621 tggtggagag acgttgaaaa ctattcaaaa acagaattcc gtcctggtca ttgaatccac
     1681 cactcaagaa aaaacgtgtt ttatctgttc ctccggttgc acttatatca accatacgtg
     1741 ttttggcctc ttcgttaaaa gccagtttct cccagttcgt gtttgtttcc aggtttctcc
     1801 aattggaaaa accggaataa cgggtcagcc ggttttcagc aaattccagc caggaaccgc
     1861 catattgagc gggatccagc ggatccacgc cttcaataac atcactgttg atagccgctt
     1921 cacgaagcaa atccacgtaa tttttagcat ccaggaaacc gcggtaacca gtaggcttat
     1981 ttgtaccata ctggaagttg acattcagtt gtgttttccc gatttttcct tttttggtgg
     2041 taatcagcac caccccatta gccgcgcggg aaccatatat agccgccgct gaagcgtctt
     2101 tcagaatatc aaaagtttca atatcattga agttgatatc tgccagatta tttccggaat
     2161 taccatcccc attaatagga attccatcaa ccacatataa tggtgtattt gaagcagaaa
     2221 tagaacctgc tccacggatc cgtactttga taccttcccc gaccttacca ctttgggatt
     2281 ccacaaacac gccggcggca cgtccctgga tcgcctgggt aaagttggct accggtgtat
     2341 tggctacttc agatccttta atacgggcaa tattaccagt aaggtccttt ttaatcttgg
     2401 tgccataacc gaccacaatc acctcatcca tgctgcggga gtcgccggcc gttaaagaaa
     2461 tagtcaaagg agctccggtg atggcaattt cccgtgtttc aaagccaatg gctgaaacaa
     2521 caagggattt tgtatttgca tccaccgtca gggtaaatgt tccatcatta gctgtggctg
     2581 ttccgtttct tgaaccttta gcggttactg aaacattcga taaagggctt ccacccgcgt
     2641 ctgtaacctt tccggttacc tctctgttct gagctgaaag gatttgggct gaaaaaagca
     2701 caaatccaag caagagcagt cttagtttac tcattgtgtt ggtttttttg aaatataaga
     2761 agggataaat atatgttaac cttgtcttaa caaaacaaga aaaccatgca gattgctgca
     2821 tggttttgaa aattgattgc gatcaacgaa caggtgttaa ctcctgtccc ggctccccat
     2881 atagatcatt atatattgta tcagttgtac caccgctgcc agggccgcca ctacataagt
     2941 ggttgcagcc cacttcaaag catccttggc tttgggaaat tcaacggaat tggtaatatt
     3001 agtctgctcc agccaggcca gggctctttt acttgcatca aactccacag ggagggttac
     3061 cagggtaaac aaaacggtaa cagccataat gataatacct accaacagga tggttggatt
     3121 cttggtactg gccagtacca ccatacctgc cagtaaaatc cagtttacca gcatggaact
     3181 ccactgaacc gcgggcacca ttttgctacg gaacatcagg ggaccgtatt gagtggcatg
     3241 ttgtacggcg tgaccacatt catgggcagc taccgcggca gcggaaatac tggttccgtt
     3301 aaatacctcc ggactcaggt tcactgtccg gtttaccggg ttatagtgat cagaaagaaa
     3361 accgtcaaca gatataactt ttacatcata tattgcgttc tccctgagca ttttctcggc
     3421 aatttcacgg ccggaaagac cgctggcgag cccaatttta ctatactttt tgaacttact
     3481 tttgagcacc gcagagacca ggaaactgag tcccagaaag accaacgaaa cgataaaaat
     3541 ctcacttgtc atggcttaaa aattattgat ttgagtatag gttataacaa atttgcgacc
     3601 atttggatca gtccggtttt tttcagtcgt tttgtcattc cccgctaaaa aaccgctctt
     3661 tttttgattg gcttattaaa gataggactt tttatttcta tcagataact atttaacaat
     3721 caattaatta aaaacataaa ctaattgatt caacgggtaa tttatagcac ctgacaggcc
     3781 ttttaagtta ttcacagcag caaaaattta ttttgaaatg tggggcataa ttgtaactta
     3841 gcaatagaat ttaatgggtt agtaagtaag agggtcagtc gtcagattgg ccctttttac
     3901 ttttcaggaa cgcctgcctt tttttctctc ttttcttttt ataaacctgc ctttttttta
     3961 tctggattta atcaattggt tgtttgatca tcgatccttt ctgcttgcag ttccaaatca
     4021 tttaatttta tcgcatgagt aaagcaaaat caacattttt ttgtcagcaa tgcggacatg
     4081 aaagtgtgaa atgggcaggt cagtgtcctt catgcggaca atggaatacg tttgtcgaag
     4141 aactggtgca aaaagataac gggaaaaata atccgggatg gaaagattac aactccgaaa
     4201 aaagaaccgc gaaaaccgta tcactgaatg aaataaaaaa tacggaagaa cgcagactgc
     4261 cgactgccga tgcagaactg aaccgtgtac tcggtggtgg cattgttcca ggcagtctgg
     4321 tattagtagc cggcgaacca ggcattggta aatcaacctt gtttttacaa aacggtttat
     4381 ggctgaaaga aattgtggtg ctctacatca gtggcgaaga aagtgaacaa caactgaaga
     4441 tgcgggccga ccggctcgga ttaaagaatg atcagtttta cctgctgacc gaaacatcca
     4501 cacagattat tttccaggag atcaaaaaac tgaaacccca actggtaatt gtagactcta
     4561 tccagacctt acacaccacc catattgaat cttcccccgg aagtgtatca cagatccggg
     4621 aatgtaccgc cgagtttcaa cgcttcgcca aagaaaccaa tacacccgtg tttctcattg
     4681 ggcatattac caaagatggc agtattgccg gacctaagat tcttgaacat atggtggata
     4741 ccgttctgca gtttgaaggg gatcgtcatt atgcctaccg tatcctgcgt acattaaaaa
     4801 atcgtttcgg cagtaccgct gaactgggta tttatgaaat gactgattcc ggtatgaggg
     4861 gtgtactcaa tccgagtgaa atactgatca cgcagaaaga agaccagttg agtgggattg
     4921 cgatagccgc caccattgaa gggatgcgtc ccctgctgat cgaagtgcag gccctcgtaa
     4981 cgcaatcggt atacggtact ccgcaacgga ccgtcagtgg ttttgacctg cgccgtttgc
     5041 aattattatt ggccgtgctt gagaaaagag gtggcttcca tttcggtatg aaagatgtct
     5101 tcctgaatat tgccggtggt atcaaagtgg aagacccctc catcgacctc gctgtacttt
     5161 gtgccctcct ctcttcctat gaagatgtaa ccctgccaca acatatctgt tttgccggcg
     5221 aagtgggact gagtggcgag atccgtgctg tgaaccggat tgaacaacgg attgcggaag
     5281 cggaaaaact tggatttgaa aaaatcctgg tttccaaata caaccagaag gggctggcca
     5341 aacagaaatt taatatagaa gtagtgacca tgggtcgcgt ggaggaggtg taccggtatt
     5401 tattctgagt cttttcttag atttacggtc caaaccttat cactatgttg ttattaaaag
     5461 aaatgaagga ttccctcctt cacttcctct ttccgcatac ctgctgtgga tgtggcaccg
     5521 atatattacc ggaagactcc accctttgcc tgcgttgcct gcatgcatta cctgagactt
     5581 gttttgaatg gcatccctcc aatccggcag agaaaatgtt ctggggaaga ttgcccctgc
     5641 aaagtgccag tgctacttac tatttcacca aggaatcgct ggtacaacac ctgatgcacc
     5701 agctgaagta caagggcaat aaagaactgg gagtacaact gggaatgctt atgggggagg
     5761 ccttgaaaaa atcgggacgg tttccggctg atgcactgat cccacttccc ttattcccag
     5821 ccaaagaaaa aagaagggga tataaccagg ccaccatact ctgtgaagga atctcggcgg
     5881 tgctgcatat tcccgtgtgg gatcaggtag tgaaacgacc ggaacatacc gaaacgcaga
     5941 ccaaaaaagg aagggtggaa cgatggaaga atatggaagg gaaatttata gtgcaggaca
     6001 ccccccttct gcaaaataaa agactgctgc ttgtggatga tgtggtgacc accggtgcca
     6061 ccctggaatc ctgcggggcg gaattattaa aaatacccgg agtacaactg agtattgcca
     6121 ccttatgcat tgccgctcgt taatgtcaga ttcatggctt atttttgaat ccaatgaagt
     6181 tacccggact cctcgttata tccctgctcc tcttgttgca ttcctgccgg aaagattcct
     6241 ttattacctc gcctgaagcc cggatcggac tgagcataga tacattgaaa tttgatacgg
     6301 tatttgtcag tgccggttcc actttccgca cactgattat caaaaacgag aatgaccagc
     6361 aactgcggct ctcttccctc aaattgatgg gtggcaatgc ctccgtattt aaaatgaatg
     6421 tggatggtac accaggccac cagttcagca acctggatat tgcggccaat gacagtcttt
     6481 ttgtatttat acaggtcaat gtaaatccca atgccgccaa cctgcccttt gtgatacgcg
     6541 acagtataca gattgaattt aacggcaaca ataaaaaagt acaactggaa gcctggggcc
     6601 gcaatgccca tttctaccgc gaccgggtga tcagcaatga tgagtcctgg aacaatgatc
     6661 tgccttatgt gatattgggt tcactggtag taccggatgg aaaaacactc acgatcaata
     6721 aaggctgccg gatctatgtc catgccgatg cgccgctaat tgtggatggc accctgatcg
     6781 tgaacggtga aaaagatacg tccgaccggg tttatttcca gggagacagg ctggatgacc
     6841 catataaaga ttatccggcc agctggcccg gacttttctt tttatcgaat gctaaagaca
     6901 atgtgctcaa ttatgcagtg ataaaaaatg cctaccaggc catcgggatg caaggccccg
     6961 ctttgaacag caatcctaaa ttaatactta acgaaacggt gatcgataat gcgtatgatg
     7021 cggggatcat agcgatcaat tccagtctgc gggcccgcaa ttgcctgatc acgaactgcg
     7081 gaaaaaatct ttacctggta cagggtggca actaccaatt cacccattgt acggtggtaa
     7141 cctatgctaa ccggtttata gaacataaag agccggtcct ctttttatcc aatacggcca
     7201 atagtgttac caattctctg gatgcgatct tccgcaattg tattttctgg ggagagaacg
     7261 gagtggtgac agatgaagtg gccgtagaaa aatccggcac cggcacattc aatgtcaatt
     7321 ttgattatac gctctggaaa gtacaggctc ccccggccaa cagtaccatt acccaggcaa
     7381 tcaataatca gacacccctg tttgacagta ttaatacctc cgacaattat tatgatttcc
     7441 ggctcaaaac cggttccccg gccctgaata aaggagtcaa tgccgggata ttcactgact
     7501 tagacgggaa aatccgaccg gtaggactgc cggacctggg cagtttcgaa aagcaataag
     7561 caaaccattc ctcctttctt tccgtttgga gaaccagacg gccttatttt tgttattcaa
     7621 cttattaaac cagcgaaaca tgaaagcatt agtcatcacc cttattatga gtaccttttt
     7681 aacagccggt tctatctatg attttaaagt gactggtctg gatggaaaag acattgacct
     7741 ctcagcctac aaaggcaaaa agatcctgat cgtgaatacc gcatctaaat gcggactaac
     7801 accccagttt gaaggattgg aagcattata taaaaaatat aagggcaaac tggtaatcct
     7861 cggtttcccc gccaataatt tcgccggaca ggaacccaaa agcaatgaag agatcggtga
     7921 attttgtaaa cagaactatg gagtaacctt cccgatggct gaaaaaattt cagtaaaagg
     7981 cgatgacacc catgccctct acaaattcct gaaagagcag gccgctgcca aaggctttgc
     8041 cgatccggta acctggaatt ttggcaagtt tctgatcaat gagaaaggcg aactgatcgc
     8101 caccttctcc ccaaagacaa caccgatgag tgatgaaatt ttaaaatacc tgaattaata
     8161 caccggacgg taaacaaggt gatacgaaag acctgtgcca tgtggtgcag gttttttttt
     8221 gataattgag cattgtattt cttagatacg aaatgttcaa tgctcaattt tcaatagttg
     8281 aaccccttcg gggttccagg ggccgtgggg gcacgcatac cccgggtttc acccggggct
     8341 att
//
LOCUS       DAOPMW010000118         8341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9492_length_8341_cov_7.137702, whole genome shotgun sequence.
ACCESSION   DAOPMW010000118 DAOPMW010000000
VERSION     DAOPMW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_8341_cov_7.137702"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(457..768)
                     /locus_tag="PLU11_07230"
     CDS             complement(457..768)
                     /locus_tag="PLU11_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPN58947.1"
                     /translation="MKNGHNYYVYIVQCSDGKYYTGVTNDVDRRLWEHNTGFNIDCFT
                     YKRRPVVLKYDEHFTDINNAIAWEKQIKGWSRAKKEALFLRNWKLISELAKSKGEREK
                     K"
     gene            complement(1316..2239)
                     /locus_tag="PLU11_07235"
     CDS             complement(1316..2239)
                     /locus_tag="PLU11_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPN58948.1"
                     /translation="MKKIVFAILSFFSMVSLSAQSDSVWLFSYFKNNGQDGLHFASST
                     DGLKWKALKNDSSFLTPTVSKDKLMRDPCILRGQDGKFHMVWTVSWNDRGIGYASSDD
                     LVHWSEQQFIPVMMHEDSARNCWAPEITWDRKKKQYMIYWATTIRGKYPEDPKVESGY
                     NHRMYYVTTKDFKSFSKTKMLYDKGFNVIDASILPVGKKYVMFLKDETRTPPQKNIRV
                     AFSKKLTSGFSAPSAPITGNYWAEGPTALYKDGQWIVYFDKYRDHKYGAISSPDLINW
                     TDISDQISIPAGLRHGTIFRVSKAELDVMLR"
     gene            complement(2245..5700)
                     /locus_tag="PLU11_07240"
     CDS             complement(2245..5700)
                     /locus_tag="PLU11_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HPN58949.1"
                     /translation="MSKSLISGLLLLCFLQLNRVAVAQPGDSLRLWHNKERKVHYLPD
                     GKDFVCVNPHLRFNRALYGTNTAFRVEAGDLPEFALYMPGMGGNLRFALINKEKHKWL
                     IESKEINTRYCPGSMIYEIKDPLLGEGTLTITILALSAAEGLVIRINGSQLPADLQLA
                     WVFGGASGKKFSRDGDIGADPESSFYLKAENCKDNSYRINGNSFLLSYGTGKVLTEEE
                     RYEIQTKPESQLKGEIPANAKQLTGYFPVSSTVRLMSAANLNNIFSSADTSKATPCIT
                     GTFLPGKGDDFFMIHNSATSVYRVAADEFAEAEKARQELAGRIEVRTPDSFLNTLGGA
                     LSLAADAIWEDPSFMHGAVAWRMRLPGWRGAYTADVLGWHDRARRNFESYALSQITTP
                     ATTGVVMDTALNLARHLEKVGTQLFSEGYISRNPNGDIRAHHYDMNLVFIDQLLNHFK
                     YTGDTAFVKKMWPLLVRHLAWETNNFDMDGDGLYDAYAAIWASDAMQYSGGGVTHSSA
                     YNFKAFKEAAALAKLIGVDGTAYEEEADKILKAMNQQLWMKDKGWYAEYKDLLGNKLL
                     HPAAGLWTIYHALDSKVPDPFQAYQSLRYIDNHIPRIPVIAKGWNEKGLYLYSETNWQ
                     PYTWSLNNVVTSENLHTALAYWQGNRKEEAFKLWRSAMLETMYLSAGPGNFQQLSFYD
                     AIRGELYRDFADGIGMVSRSLVEGLFGINPNGLHNELVITPGFPQNWNEAGLTTPDIR
                     FDFKRDGDQDLYQIRQFHTARTVKLILPAFKDRLLNVMVNGKMIKWKQVPDAVGQPLV
                     ELDLPADDSLSVSLTWTGQQPDRFIIKNQYAAGEPFSVQSKQLSKYKLFDPQRILVRA
                     FTDKQAAGGRLSYKPGHHTLFVRTTQGAFTWWQPVDIYITPVVPVVETRPVDMNAIKD
                     KVELGSVFNDKVTHIFKPQYKSPRPVSPTLQLPIQGIGNWAYHSVQFNVIDSGFRARA
                     GAKNEWTSSTGMSFNTPSDTSKNNIVFTSMWDVYPDSVMIPLTGKASEVAFLMAGSTN
                     PMQSRLVNGLIRVNYTDGSSDVLELRNPENWWPIEQDYYVDGFVFTTDAPKPIRVSLK
                     SGTELPPNYKYTGIKGFSNFGIEGGAATVLGMTLNKNKTLRSLVLKAVANDVVIGLMG
                     LTLIR"
     gene            complement(5693..6388)
                     /locus_tag="PLU11_07245"
     CDS             complement(5693..6388)
                     /locus_tag="PLU11_07245"
                     /inference="COORDINATES: protein motif:HMM:NF039702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HPN58950.1"
                     /translation="MKRAARYGTGMIIMVGLLSLQQQQSVTKEVLLFSDDFAGKESAG
                     NYIIEKNSKDTNAVQFMGEQLQLDSEGGLTVWYREKLKGDIRISFDRMVVMEAGPHDR
                     LSDLNQFWMANDPGEKMFKRTGGFREYDSLLMYYVGMGGNYNKTTRMRRYDGKGNLEI
                     VGEYTDSAHLLKPNHWYHIDIITRKGINSFIVDGIKYFEYEDKNQIGSGWFAFRSTRS
                     RQRIDNLKIYALE"
     gene            complement(6385..8085)
                     /locus_tag="PLU11_07250"
     CDS             complement(6385..8085)
                     /locus_tag="PLU11_07250"
                     /inference="COORDINATES: protein motif:HMM:NF026034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HPN58951.1"
                     /translation="MKRISIIFSLLLLPAVLLRAQVRILEEGNTVSLSNPIVSISFDK
                     NNADLNKIIYKGKSLLNGKDQRGYLLGPGFSMYPSVFKVIRSSEALVELSFYHEASNH
                     FQYDLRYILRANDPGIYCYLVQTHRAADSVGNFGQTRWGIRSDEALFDYHLVRDSIQG
                     PMPKMAELKEEIQDWTFKMADGRIYTKYNYADYIEGRHVHGMAGQQSGLGLFTIQASH
                     EYLNGGPTKQYQNVHSNPYLINMFNCGHFLSDKRKGDNLITDDWTKLNGPFFLYFNEG
                     KSINDIWADAKKRAAEEISQWPYEWMQHEAYPLERGSVSGIVLSEGKPVANAMVILAA
                     PDFDWQAQSRGYQYYTRSGADGRFVVEKVRPGSYTLYMYGNNTTTDYRKDKITVGEGM
                     TTMLGKINWPVSTGKHVLWQIGVADRKTSGFRLADRPRDYSLFKLPPENLKFFPGIQL
                     DSDWYYAQTKRGSWDIHFTLERAYKDTATLTVGIAGSAKNPAWEVWLNERKLGVWQFG
                     NDASVYRSAVAGGYYVKQEIRFPAGEFREGENIISFRLPDVKDGGGIMYDVIKLEVKQ
                     "
     gene            complement(8100..>8341)
                     /locus_tag="PLU11_07255"
     CDS             complement(8100..>8341)
                     /locus_tag="PLU11_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58952.1"
                     /translation="HRPEDAVNALLMPVRTNTYLINGHNYQDERLTIYLPGNGGLLNA
                     VALMCTGADADKEKNIGFPKDGTWKVRWEGLKKMF"
BASE COUNT         2056 a         2152 c         1872 g         2261 t
ORIGIN      
        1 cacgctcacc atgacaatgg ttcgataaac tcaccatgac aagggcaccg gctgctttac
       61 cggaacgaag gggacagtcc gttgtcaccc tgagcgtgtc gaagggtggc gaagtaagtg
      121 cccatgggtt ggtgcatggt tcgacacgct caccatgaca acggctgtca ccctgagctt
      181 gccgaagggc gacgtagcaa gtgccctttg gttggtgcat ggttcggcac gctcaccatg
      241 acaatggttc gataaactca ccatgacaag ggcaccggct gctttaccgg aacgaagggg
      301 acagtccgtt gtcaccctga gcgtgtcgaa gggtggcgaa gtaagtgccc atgggttggt
      361 gcatggttcg gcacgctcac catgacaacg gactccctca ccatgacaac ggactccctc
      421 tctatgacta cggccccctc accattacaa caactgtcat ttcttctctc tctccccttt
      481 tgactttgct agctcagaaa tcaacttcca gtttcgtaga aataatgctt ccttttttgc
      541 cctggaccag ccctttatct gcttttccca ggcaatcgca ttattaatgt ctgtaaagtg
      601 ttcatcatac tttaaaacta ctggtcgtcg cttataggta aagcagtcaa tattaaaacc
      661 agtattgtgc tcccatagcc ttctatctac atcgttggtt acccccgtat agtacttgcc
      721 atcggaacat tgaactatgt atacatagta gttatgtcca ttcttcataa acaaggttta
      781 attaagaaat gccggaatat cgggaaaagt tgaattgaaa aaataaccgt cgcgggcgaa
      841 ttttctttgg ttggttcatg gttcggcaag ctcaccatga caacagctgt caccctgagc
      901 gtgtcgaagg gtgaggaaga caaagcactt tttgctattc atggttcggc aagctcacca
      961 tgacaagagc accggttgct ttaccggtaa gtagggggca gcccactgtc acccagagcc
     1021 tgccgaaggg cgacgaagaa agtgcgcttt ggttgttgca tggttcgaca cgctcaccat
     1081 gacaacagca ccggttgctt taccggtacg atggagactg ttcgctgtca ccctgagcct
     1141 gccgaagggc gacgaagaaa gtgctctttg gttggtgctg gttcgacacg ctcaccatga
     1201 caaggaccgt ttcttctttc ccggtaaaac aagggacagc tcgctgtcac cctgagcttg
     1261 tcgaagggcg acacgctcac catgacaacg gactccctca ccatgacaac agctgtcacc
     1321 tcagcatgac atcgagctct gccttactta ccctgaaaat cgtaccatgc cgtaaacctg
     1381 ctggtatact gatctgatcg gagatatcgg tccagtttat caggtcgggg gaactgatgg
     1441 ccccatattt atgatcgcgg tatttgtcga agtagacgat ccattggccg tctttgtaga
     1501 gggcggttgg tccctcggcc cagtagttgc cggtgatggg ggctgaggga gcggaaaagc
     1561 cactggtcag ttttttactg aaagctaccc ggatattctt ctgcggagga gtacgggttt
     1621 cgtctttgag gaacatcaca tattttttcc cgacgggcag gatgctggca tcaatcacat
     1681 tgaagccttt gtcgtagagc atttttgttt tactgaagga cttaaaatcc ttcgtggtca
     1741 cataatacat ccggtgatta tacccactct ccaccttggg atcttccggg tacttgcccc
     1801 ggatggtggt ggcccagtaa atcatgtatt gttttttctt ccggtcccag gtgatctccg
     1861 gtgcccagca gttacgggcc gaatcctcgt gcatcatcac gggaatgaat tgctgttcgc
     1921 tccagtgtac caggtcatcc gaactggcat acccaatccc ccggtcgttc cagctcacgg
     1981 tccataccat gtggaatttg ccgtcctgtc cgcggaggat acaggggtca cgcatgagtt
     2041 tatctttcga aaccgttggt gtaagaaaag aactgtcatt tttgagtgct ttccatttca
     2101 atccatcggt actggaggcg aaatgcaatc cgtcctggcc gttgttttta aaataggaaa
     2161 agagccagac actgtctgat tgggcagaaa gcgataccat cgaaaaaaaa ctcagaattg
     2221 cgaacactat ttttttcata ctcattatct gattaaagtc aaccccatca acccgatcac
     2281 cacatcattg gccacggctt tgagcaccag gctgcggagg gttttgtttt tattcaatgt
     2341 catgcccagt acggtggccg cacctccttc aatcccaaaa ttagaaaagc ctttgatccc
     2401 ggtgtattta taattgggag gtagctcagt acccgacttc aacgaaaccc gtatcggttt
     2461 gggcgcatcg gtggtaaaca caaatccatc cacataataa tcctgttcaa tcggccacca
     2521 gttctccgga ttgcggagtt cgagtacatc ggaacttcca tcggtatagt ttacccggat
     2581 caacccattg accaaccgac tctgcatcgg attagtgctg ccggccatca ggaaagccac
     2641 ttcagaagct tttcctgtta aggggatcat gactgaatcc ggatacacat cccacataga
     2701 cgtgaaaaca atattgttct ttgacgtatc ggaaggtgta ttgaaggaca tacctgttga
     2761 actcgtccat tcattttttg ctcctgccct cgcgcggaat ccgctgtcta ttacattaaa
     2821 ctgcacacta tggtaggccc agttccctat tccctgtatg ggcaactgaa gtgtgggcga
     2881 aacaggtctg ggtgatttgt actgcggttt gaatatatgg gtcactttat cattgaaaac
     2941 agatccgagt tcaactttat cctttatggc attcatatca accggccggg tttcaacgac
     3001 aggcacaacc ggtgtaatat aaatatccac cggctgccac caggtgaatg caccctgggt
     3061 ggttcttaca aaaagtgtgt gatgcccggg cttataggat aaccttcccc ccgccgcttg
     3121 tttatcggta aaagctctta ccaaaatccg ctggggatca aacagtttat atttactcaa
     3181 ttgcttggac tgtacggaaa atggttctcc ggctgcatac tggttcttta taatgaaccg
     3241 atccggctgc tgccctgtcc atgtcagtga aaccgataaa ctatcatctg ccggtaagtc
     3301 caattccact aatggttgtc ccacggcatc cggcacctgt ttccatttga tcatcttccc
     3361 attgaccatc acgttcagta agcgatcttt aaatgccggc agaatcagtt tcacggtacg
     3421 ggctgtatgg aactgacgta tctggtatag gtcctgatct ccatcgcgtt tgaaatcaaa
     3481 acggatgtcc ggggtggtca gtcctgcttc attccagttt tgcggaaaac ccggtgtaat
     3541 caccagttca ttgtgcaagc cgttggggtt aattccaaac aatccttcta ccaaactgcg
     3601 ggagaccatt ccaataccat cggcaaaatc acgatagagt tcaccacgga tggcgtcata
     3661 aaaactcagc tgttgaaaat tacccggacc ggcactcagg tacatggttt ccagcatggc
     3721 actgcgccag agtttgaaag cttcttcctt gcggttaccc tgccaataag cgagggcggt
     3781 atgcagattc tcactggtca ccacattatt cagactccag gtatagggct gccagttggt
     3841 ttcactgtag agatacaaac ctttctcatt ccagcctttg gcgatgacgg gtatacgggg
     3901 aatatgatta tcgatatacc gtagcgactg ataagcctga aaaggatccg gtactttact
     3961 gtcgagggca tgatatatag tccagagtcc ggcagccgga tgcagtaatt tatttcctaa
     4021 aaggtctttg tattccgcat accagccctt gtccttcatc cacaactgct ggttcatcgc
     4081 tttgagaatt ttatccgcct cttcctcata agccgtacca tccaccccaa tcagtttagc
     4141 gagggcagct gcttctttga atgccttgaa attatacgca ctgctatgcg tcacgcctcc
     4201 cccgctgtac tgcatggcat cactggccca gatggcggca taagcatcat agagcccgtc
     4261 gccatccata tcaaagttat tggtctccca ggccaggtgt cttaccagca ggggccacat
     4321 tttctttaca aaagcagtgt cgcctgtgta tttgaaatga tttagtaact gatcaataaa
     4381 aaccaggttc atatcatagt ggtgcgcccg gatatcgcca ttgggattac ggctgatata
     4441 gccctcactg aataactggg tacccacttt ctcgagatga cgggccaggt tcaatgcggt
     4501 atccatcacc accccggtgg tagcgggtgt agtgatctgc gataaagcgt aggattcaaa
     4561 atttcttctg gccctgtcgt gccagccgag tacatcggca gtatacgcgc ctctccatcc
     4621 gggtaaacgc atccgccagg ccacagctcc atgcatgaaa gaaggatctt cccagatggc
     4681 atcggcggct aaggataagg caccgcccaa tgtattgaga aaagaatcgg gggtacgtac
     4741 ttctatcctg ccagccagtt cctgacgggc tttctctgct tcggcaaatt catcggctgc
     4801 tacgcggtat actgatgtag ccgaattatg aatcataaaa aaatcatcgc ctttacctgg
     4861 caagaaagtc ccggtaatac agggcgttgc tttcgaagta tctgcacttg aaaatatatt
     4921 attcaggttg gccgcactca tcagtctgac ggtggacgat accggaaaat aaccggtcag
     4981 ttgttttgca ttagcaggta tctctccttt caactgactc tccggtttgg tttgtatctc
     5041 atagcgctct tcttcggtca gtacttttcc cgtgccgtag gaaagcagaa aagaatttcc
     5101 attgatgcgg taactgttat ccttacaatt ctccgctttc aggtaaaaag aggattcagg
     5161 atcagcaccg atatcaccat cccgtgagaa tttttttccg gatgcaccac cgaataccca
     5221 ggccaattgg aggtcagccg gcaattgact accattgata cgaatcacca atccttctgc
     5281 agcggacaaa gccagtatcg taattgttaa tgtcccttca cccagcaatg gatcttttat
     5341 ttcatagatc attgaaccgg gacaataacg ggtattgatc tcttttgatt cgatcagcca
     5401 tttatgtttt tccttattga tcagggcaaa acgcagattg ccccccatac ccggcatata
     5461 caatgcaaat tcaggcaaat cacccgcttc caccctgaat gccgtattgg tgccatagag
     5521 cgcccggttg aaacgcaggt gcggattcac acagacaaaa tctttaccat ccggcaggta
     5581 atggactttt cgttctttat tatgccataa ccggagactg tcaccgggct gtgccaccgc
     5641 aacacggttc agctgcagaa agcagagcag cagtaagccg gaaattaatg atttactcaa
     5701 gtgcataaat tttcaggtta tcaatccgtt gccggctcct ggtactgcgg aaagcaaacc
     5761 agccggaacc gatctgattt ttgtcttcgt attcaaaata ctttatccca tccacgataa
     5821 aactattgat ccccttgcgg gtgatgatgt caatatggta ccagtgattg ggtttcagca
     5881 aatgtgcact gtcggtgtac tcgcccacta tttccaggtt ccctttccca tcatagcgcc
     5941 gcatcctcgt ggttttatta taattgccgc ccatgcccac atagtacatc agcagactat
     6001 cgtattcacg gaaaccaccg gtacgtttaa acattttttc acccggatca ttggccatcc
     6061 agaactgatt caggtcactg agccggtcat gcggaccagc ttccattaca accatccggt
     6121 caaaggaaat acggatatcg cctttcagtt tctcccggta ccatacggtc agcccgcctt
     6181 ctgaatcgag ttgcagttgt tcccccatga attgtactgc attggtatcc ttgctgtttt
     6241 tctcaatgat ataattacca gctgattctt taccagcgaa atcatcactg aaaagcaata
     6301 cttcttttgt tacggattgc tgttgctgaa gagaaagcaa tcccaccatg atgatcattc
     6361 ctgttccata tcgtgctgca cgcttcattg ttttacttcc agtttaatca catcatacat
     6421 gatcccgcca ccgtctttga catccggtaa ccggaatgaa ataatatttt ctccttcccg
     6481 gaattcaccg gccggaaaac gtatctcttg ttttacataa taacccccag ccactgcgct
     6541 gcgatataca ctggcatcat tgccaaactg ccagaccccg agcttacgct cattgagcca
     6601 gacttcccag gctggattct ttgcactgcc agcgataccc accgtaagtg tagctgtatc
     6661 cttgtaggct ctttccaagg tgaaatgaat atcccaactg cctcttttgg tctgtgcata
     6721 ataccagtcg ctgtcaagct gaatgccggg aaaaaatttc aggttctccg ggggcagttt
     6781 aaaaagacta tagtctctcg gacgatccgc taaacgaaaa ccgcttgtct tccggtcggc
     6841 caccccgatc tgccagagta catgcttccc ggtcgacacc ggccagttga tcttacccag
     6901 catggtggtc atcccttcac cgactgttat cttatctttt ctgtaatcag tcgttgtgtt
     6961 attcccgtac atgtaaagtg tataagaacc gggcctgact ttctccacta caaatcgtcc
     7021 atccgccccg ctgcgggtat aatactggta cccgcggctt tgtgcctgcc agtcaaaatc
     7081 aggagcagca aggatgacca ttgcattggc tacgggctta ccctccgaca aaacaatccc
     7141 gctcacagaa ccccgttcca acggataggc ttcatgctgc atccattcat aaggccattg
     7201 actgatttct tctgcggctc tttttttggc atcggcccaa atatcattga ttgattttcc
     7261 ttcattaaaa tataaaaaga aggggccgtt cagtttcgtc cagtcatctg tgatcaggtt
     7321 atcccccttc cgtttatcac tgagaaaatg gccacagttg aacatgttga tgagataggg
     7381 attgctgtgg acattctggt attgcttggt gggaccgcca ttgaggtatt catgactggc
     7441 ctggatggta aatagtccga gtcctgattg ttgtccggcc atcccatgca catgccggcc
     7501 ttctatataa tccgcataat tgtatttggt atagatacgg ccatcggcca ttttaaaggt
     7561 ccagtcctgt atctcttctt tcagttccgc catctttggc atgggacctt gtatactatc
     7621 ccgcaccagg tgataatcaa acaaggcttc gtcactacgg atgccccagc gggtctggcc
     7681 gaaattacca acagagtccg cggcccggtg cgtttgtacg aggtaacaat aaatacccgg
     7741 atcattggcg cgtagaatat accggagatc gtactgaaaa tgattggaag cttcgtgata
     7801 aaaggagagt tccaccagtg cctccgaaga acggatcact ttaaacacgg atggatacat
     7861 ggaaaatccg gggccgagca ggtagccccg ctggtctttg ccattcagca gacttttccc
     7921 tttgtagata atcttattca gatccgcatt gtttttatca aaggagatac tgactatggg
     7981 gttggacaaa ctaaccgtat tgccttcttc gaggatacgc acctgtgccc ggagcagtac
     8041 tgccggcagc aggagtaatg agaaaattat actgatacgt ttcatatagc ctaatcgcat
     8101 taaaacattt tcttcaaccc ttcccatctt actttccagg taccatcttt cgggaaacca
     8161 atattttttt ctttatctgc atcggcgccg gtacacatca gggcgacagc atttaataaa
     8221 ccgccattcc ccggcagata aatagtcagc cgttcatcct ggtaattatg tccgttgatc
     8281 agataggtat tcgtgcgcac gggcatcagc agggcattca ccgcatcttc cggacgatgc
     8341 a
//
LOCUS       DAOPMW010000119         8195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9718_length_8195_cov_8.085749, whole genome shotgun sequence.
ACCESSION   DAOPMW010000119 DAOPMW010000000
VERSION     DAOPMW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9718_length_8195_cov_8.085749"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLU11_07260"
     CDS             complement(<1..635)
                     /locus_tag="PLU11_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPN58953.1"
                     /translation="MKKTILLLLVLIASLAFVPQKKSKVIFFGDSITELGVKPGGYVT
                     RVDSMSKAEGKGGSFEFAGAGVSGNKVYDLYLRMEEDVLAKNPDIVVIYVGVNDVWHK
                     TLLGTGTDPDKFEKFYSAILRKLNEKNIKAVLCTPAVVGEKTDMSNPLDGDLNRYSNI
                     IRDIAKKNNLPLIDLRKKFIDYELANNPENKEKEILTYDRVHMNAKGNQFLA"
     gene            complement(717..2645)
                     /locus_tag="PLU11_07265"
     CDS             complement(717..2645)
                     /locus_tag="PLU11_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN58954.1"
                     /translation="MKLFQKTMLVLSAFVFLLTAACNTKAKEEKPPVIPVENFFKNPE
                     NFGWQLSPDGAYISYLSPHMGHTNVFVRKITDTTGVPVTHDSTRNIYDYRWKGSRILF
                     LQDVGGDENFQLFSVGMDGKDTKALTPFPKVRTGILDDLRYVPGKEKEVIVQLNKRDP
                     RFMDPYSINIETGEMKVLYQNDKNFDGWYTDHTGLIRIASKTDGVNTTYYHRATETTP
                     FDSLLTTTYKDNFGIQLFTFDNKNLYATSNIGRDKTAVIEYDLAARKEVKELYTNTDY
                     DVDGLSWSAKRKVLESVNFTSWKGEHHFLDKEAEAEYTKMKEKFSGYEVGLYGNNDAE
                     DKFVVWTGNDKLAAKYYFYDKKSGETKFLADAFPWLKEGDMAARKAITYQSRDGLTIH
                     GYLTLPKGMDPENLPVVINPHGGPWARDNWGYNGEIQFLANRGYAVLQMNFRGSTGYG
                     KEFWLKGAKQWGKTMQDDITDGVQWLIKEGIADPKRVAIYGASYGGYATLAGVTFTPD
                     LYACAVDYVGVSNMFTFMKTIPPYWEPYKAMFQELVGDPVKDSLLLAEVSPALHAERI
                     KVPLFIAQGANDPRVNKAESDQMVEALKKRGVEVEYMVKDDEGHGFANEKNQIDFYKA
                     MEGFFAKHLAAPAAAEKK"
     gene            complement(2805..3701)
                     /locus_tag="PLU11_07270"
     CDS             complement(2805..3701)
                     /locus_tag="PLU11_07270"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPN58955.1"
                     /translation="MSHPKGKLIAIGGAEDKGTDLENGEFRRNNLNFFELGILRRVVE
                     EAGGVNSRIEVITTASTIPYEVGENYLNAFGKIGCTNIGLLHIRNRQDTQIEEYLERI
                     RVCDAVMFSGGNQLRLSVTDGGTEFLNILKKRYQEEERFVIAGTSAGAMAMSSTMIYE
                     GNATRAHLKGEVKITTGLGFMSNVIIDSHFEKRGRFGRLAQAVAGNPGCIGIGLGEDT
                     GMLVTGGNRMEAIGSGLVVIVDGHEIRHSNIADIPDGNPISIENMKVHFCEKGNGYLI
                     KEREYLPEASDGSVIRKQVDVE"
     gene            complement(3986..5290)
                     /locus_tag="PLU11_07275"
     CDS             complement(3986..5290)
                     /locus_tag="PLU11_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN58956.1"
                     /translation="MSLNSRRRFLQRAGVFSATAFLTSLAKPAWSRNLDQALSQSAGI
                     SPEELATDEDFWYYIQQSFTVSPSLINLNNGGVSPAPKTVQDAMKRYYDLSNEAPSYY
                     MWRVLDQGREPLRKNLAKLAGCSPEEIAINRNSSEGLETVIFGLQLKAGDEVVAAKQD
                     YPNMINAYKQRELRDGIKMVWINLELPSEDENYLVSQFVNAFTSKTKVVHITHIINWN
                     GQILPVKKIAQEAHKRGIEVVVDGAHSFAHFDFKVPELDADYYAGSLHKWLYAPIGSG
                     VLYVRKEKIKNIYPMFAVDNPLKDDIRKFEALGTRPFFIEQAIGKAIEFHEMIGAERK
                     EKRLHYLKNYWMEKVKGLPGVKLNTSLHPKWGCAIGNVGVEGRKPGELDSFLMDKYKV
                     HTVAIEWENIKGVRVTPNVYTTTKQLDVLVEGITAFLTAKSS"
     gene            5394..8030
                     /gene="cphA"
                     /locus_tag="PLU11_07280"
     CDS             5394..8030
                     /gene="cphA"
                     /locus_tag="PLU11_07280"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPN58957.1"
                     /translation="MTILEIKVLKGPNYWSVRRTKLIQMKLDLEEKENMPTNKIPGFR
                     ERLEQLLPSLIEHRCSVGTPGGFFQRVDEGTWMGHVVEHIALEIQTLAGMDTGFGRTR
                     TPEGEKEGIYYVVFSYMEEDAGVYAAKAAVRIAQALIEGSDYDLAEDIQQMRVIREDT
                     RLGPSTGCIVEEAAKRGIPFIRLNKQSLVQLGYGVNQKRIRATIASTTSNIAVDIACD
                     KEETKMLLEAAEIPVPRGTVVRTEEGLDAAIEKFGYPLVIKPIDGNHGKGNTTNITNR
                     EQAIKAFEAAKHYSRSVIVERFITGFDFRCLVINYKFICAALRTPASVIGDGVHDLQW
                     LIEETNKDPRRGYGHEKVLTQITIDQFTQKMLDDAGVTLQHVPAKGELFLLKPTANLS
                     TGGTSSDVTDEVHPANVFMFERIAKIIGLDICGIDVMAMDLRTPVSENGGAILEVNAA
                     PGFRMHIEPAEGLPRNVAEPVVDMLFPKGSAGRIPIIAVTGTNGKTTTTRLIAHIAKT
                     AGKKTGYTTSDGVYIQNQLMMKGDCTGPISSQFVLKDPTVDFAVLECARGGILKAGLA
                     FQNCEVAVVTNVAADHIGLGGINSVEQMAKVKAVVAETVFPHGYSVLNADDDLVYKMK
                     NDLKCNIALFSMDENNPRIKAHCANHGLACVYENGYVTIMKGTWKIRVLPARDIPLTF
                     EGKAVHNINNCLPAVLAAYLYRDINIDDIRTGLQTFIPSESLTPGRLNFFHFKHFTML
                     ADFAHNPHGLRLLCDFISKLDYPAKIGVISGTGDRRDEDIRELGEISAAYFDEIIIRC
                     DKNLRGRTADEIIDLLKEGIHSVNPDIPVLTIPNEDQALDYIYENAKPGALYTIMCDV
                     VARALDKIKELKVREEKESRPA"
     gene            8119..8189
                     /locus_tag="PLU11_07285"
     tRNA            8119..8189
                     /locus_tag="PLU11_07285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8151..8153,aa:Gln,seq:ttg)
BASE COUNT         2124 a         2026 c         1808 g         2237 t
ORIGIN      
        1 gcgagaaact ggttcccttt tgcattcatg tggacccggt cgtaggtcag gatttccttt
       61 tctttattct ccgggttatt ggccagttca taatcgataa atttcttccg gagatcaatt
      121 aaggggagat tgtttttctt cgcgatgtcc cggataatat tgctgtaccg gttcaggtca
      181 ccatctaggg gattgctcat atccgtcttc tcccctacca cagcaggtgt gcaaagaacg
      241 gctttgatat ttttctcatt cagttttcta agtatagccg aatagaattt ttcaaactta
      301 tccggatccg tacccgtacc cagcagggtc ttgtgccaca catcattcac ccccacataa
      361 atcactacaa tatccggatt cttggccaat acatcctctt ccatacgcag gtacaaatca
      421 tacactttgt tcccgctgac acctgctccg gcaaattcaa aacttccgcc cttcccttct
      481 gctttactca tactgtccac ccgggtaaca tacccacccg gcttaacacc gagttctgtg
      541 atggaatcgc caaagaaaat tactttgctc ttcttttgcg gaacaaaggc aaggctggct
      601 atcagtacta aaagtaaaag gattgttttt ttcataacta acgattaata ctgcaatata
      661 aaacaaaagc accctgttag tgcaggatgc ttttggtaga attttagttg tattgtctac
      721 tttttttcag ctgcagcagg ggcagccagg tgtttggcaa aaaaaccctc catggccttg
      781 taaaaatcaa tctggttttt ctcattggca aaaccatgac cttcatcatc ttttaccata
      841 tattccactt caacaccccg cttcttcaat gcttctacca tctggtcact ttcggcttta
      901 ttgacccgtg gatcattggc tccctgggcg ataaacaacg gaactttgat ccgttctgca
      961 tgtaaagcag gcgatacttc tgccagcaat aaactgtcct tcacgggatc acccaccagt
     1021 tcctggaaca tagctttata gggttcccaa taaggcggaa tggttttcat aaatgtaaac
     1081 atgttcgata cgccaacata atccacggca caggcataca ggtcgggtgt gaaggtcacc
     1141 ccggcgagag tggcatatcc tccataactg gcaccataga ttgccactct tttgggatct
     1201 gctatccctt ctttaatcag ccactgcacg ccatccgtta tatcatcctg catggttttc
     1261 ccccactgtt tggctccttt caaccagaat tctttgccat acccggtgga accccggaaa
     1321 ttcatttgta aaacagcata cccgcggttg gccaggaatt gtatttcccc gttatacccc
     1381 cagttgtccc tggcccaggg tcccccatgt ggattgatga ccacgggcag gttttcagga
     1441 tccattcctt tgggcaaggt caggtaacca tggatagtca gcccgtccct tgattgataa
     1501 gtaatggctt tcctcgcggc catatcgcct tcttttaacc agggaaaagc atctgccagg
     1561 aatttggttt cccctgattt tttatcatag aaataatatt tagccgccag tttatcattc
     1621 ccggtccata ctacaaattt gtcttcggca tcattattcc cgtaaagtcc cacttcatat
     1681 ccgctgaatt tttccttcat cttcgtatac tccgcttcag cttccttatc caggaaatga
     1741 tgttcgcctt tccacgaagt gaagttgacg gactcgagta cttttctttt ggcagaccag
     1801 gaaagcccgt ctacatcata atctgtattg gtatataatt cttttacttc ctttctggcc
     1861 gcaaggtcat actcgatcac ggctgtttta tcccggccga tattggatgt cgcgtacagg
     1921 tttttgttat caaaagtaaa aagctggata ccgaaattgt ctttatacgt agtggtcagt
     1981 agagaatcga aaggagttgt ttccgtggcc cggtgataat aagtcgtatt caccccatct
     2041 gttttactgg cgatccggat cagcccggta tgatcggtgt accaaccatc aaagttttta
     2101 tcattctgat aaagcacttt catttccccg gtttcaatat tgatactgta gggatccata
     2161 aagcggggat cccgtttatt tagctggaca atgacttctt tttctttacc aggtacatac
     2221 cggaggtcat caagtattcc tgtccttact ttagggaaag gggttagtgc tttggtatcc
     2281 ttgccatcca tacccacaga aaacaattgg aagttttcat ccccacccac atcctgcagg
     2341 aataatatcc ggctgccttt ccagcggtag tcataaatat tacgggtgga atcatgggtt
     2401 accggcacac cggtagtatc ggtaatcttc cggacaaaaa cattggtatg acccatatgg
     2461 ggtgacaggt aagaaatgta tgctccatcc ggtgaaagct gccagccaaa attttccgga
     2521 ttcttaaaga aattctccac cggaatgacc gggggctttt cttctttggc ctttgtgtta
     2581 caggcggctg ttaacaggaa aacaaatgct gataagacca gcatcgtctt ttgaaataat
     2641 ttcattgtat ttttttaatg gttaagaaag gcgggggcgg gtttatcagt tctgaaaggg
     2701 tgggtataat gtcaagttac gataatccgg ataaaaaaag catcccgtat accggaatgc
     2761 ctttcctatt tcccattttc tatttactat ttactattcc ctaattactc cacatccacc
     2821 tgtttccgga tcacggatcc atcgctggct tccggcaggt actcccgctc tttgatcagg
     2881 taaccattgc ctttctcgca gaaatggact ttcatatttt caatactgat cggatttcca
     2941 tccgggatat ccgcaatatt actgtgcctg atttcgtgac catccacaat caccaccagt
     3001 ccgctgccaa tagcttccat ccggttgccc cctgttacca gcatgccggt atcctcccca
     3061 agcccgatac cgatacaacc gggatttcca gccacagcct gtgccagccg gccaaaccgg
     3121 cctcgttttt caaaatgcga atcaataata acattactca taaatcccag tccggtagtg
     3181 atcttcactt cgcctttcag gtgagcacgg gtcgcattcc cttcatagat catcgtactg
     3241 ctcatggcca tcgcaccggc tgaggtaccg gcaatcacaa aacgctcttc ttcctggtat
     3301 cttttcttaa ggatattgag aaactctgta cctccgtccg ttacactcag tcttaactgg
     3361 ttgcctccgg aaaacatcac cgcatcacaa acccggatcc gctccaggta ttcctcaatc
     3421 tgcgtatcct gccggttgcg gatatgcaac aacccgatat tggtacagcc gatttttcca
     3481 aatgcattca ggtagttttc ccccacttcg taaggaatgg tggaagcagt agtgatcact
     3541 tcgatccggg aattgacacc cccggcctct tccactacac gacggagaat acccaactcg
     3601 aagaaattga ggttattgcg gcggaattca ccattttcca ggtccgtgcc cttatcctcg
     3661 gcacctccaa tggcgatcag ttttccttta ggatgactca ttcatcggga tttgattcgg
     3721 acaaatgtag aaagaaaaat gtttagaaag tactatagca gaaagaggct gtaagaaggg
     3781 tttattgtgt cttaaagtag atagtggttt agattagggt ttagaaggtt tataaaggca
     3841 ttagcattaa cacgccctct tttatcctcc gaagcaattg agaggagggt ttctctcttt
     3901 ttctcttttg acctctttag tgccgcccgc agggcctaga aaagttagta gttaacagtt
     3961 cgttgttaga agtcaacaga ccttattaag aagatttagc agttaagaac gcagtaatcc
     4021 cctccactaa gacatccagc tgcttcgtcg tagtatacac attcggcgtt accctcaccc
     4081 ctttaatatt ctcccattca atagccacgg tatgcacttt gtatttgtcc atcagaaagg
     4141 agtcgagctc cccgggtttg cgcccttcta ctcccacatt accgatagca cagccccatt
     4201 tcggatgaag agaagtattg agcttgactc ccggcaagcc ttttactttt tccatccagt
     4261 aattcttcag gtaatgcagc cgcttctctt tccgctccgc ccctatcatt tcatgaaatt
     4321 caatggcttt accaatagcc tgttcaataa aaaaaggacg ggtgcccagg gcttcaaatt
     4381 ttcggatatc atccttcagc ggattatcca cggcaaacat cgggtagatg ttttttattt
     4441 tctccttccg tacataaagt actccactgc caatcggtgc ataaagccac ttatgcagac
     4501 tgccggcata ataatccgca tccagctccg gtactttaaa gtcaaaatga gcaaaactat
     4561 gcgccccgtc caccactacc tcaatccccc gcttatgggc ttcctgcgcg attttcttca
     4621 ccggcaatat ctgcccgttc cagttgatga tatgggtgat atggaccact tttgtttttg
     4681 aagtaaacgc gttaacgaac tggctcacca ggtaattttc atcctcactg ggcaattcca
     4741 ggttgatcca taccattttg atcccgtccc gtaactcccg ttgcttatag gcattgatca
     4801 tgttgggata atcctgtttt gcagctacca cttcatcacc ggctttcagt tgcaaaccaa
     4861 agatcaccgt ctccagtcct tcggaagaat tgcggttaat ggctatttcc tcgggagaac
     4921 aacccgccag cttcgccagg ttcttacgca agggttccct tccctggtcc agcacccgcc
     4981 acatataata actcggggct tcattgctca ggtcataata ccgtttcatg gcatcttgca
     5041 ctgtttttgg cgccggagaa accccgccat tattgaggtt gatcaaggaa ggagagaccg
     5101 taaaggattg ctggatataa taccagaagt cttcatcggt ggcaagttct tccggtgata
     5161 tgccggccga ctgactcaag gcctgatcca ggttacggct ccaggctggt ttggccaatg
     5221 aagtaaggaa ggcggtggcg gaaaatactc cggcccgttg caggaaacga cgacggctgt
     5281 tgagcgacat acggttgttt taggttccta aatgtactga aaaagcaatg accggaacaa
     5341 gggttggtta cctaactaaa atcacttaaa ttgacgattc aacctgactg tatatgacaa
     5401 tacttgaaat taaagtactg aaaggaccga attactggag tgtgcggcgt accaaactga
     5461 tccagatgaa actggacctc gaagagaaag agaatatgcc caccaataaa atccccggct
     5521 tccgggaacg attggaacaa ttattaccca gccttattga gcaccgttgc agtgtgggga
     5581 cccccggtgg atttttccaa cgggtggatg aaggtacctg gatgggccat gtggtggaac
     5641 atatcgcact cgaaatacaa accctggcgg gcatggatac cggcttcggc cgtacccgta
     5701 caccagaagg cgaaaaagaa ggcatttatt atgtcgtctt cagttatatg gaagaagatg
     5761 ccggcgtata tgcagccaaa gcagcggtac gtattgccca ggcattgatc gaaggttccg
     5821 attatgacct ggcagaggat attcagcaaa tgagagtgat ccgggaagac acacgcttag
     5881 gtccttccac cggttgtatt gtggaagaag ccgctaaaag agggattccc tttatacgac
     5941 tcaataaaca aagtctggtt caactgggct atggagtcaa tcagaaacgt atccgcgcca
     6001 cgattgcttc caccacgtcg aatattgcag tggatattgc ctgcgataaa gaagagacca
     6061 agatgctgct cgaagctgcg gaaattcctg tccccagagg tacagtggta agaaccgaag
     6121 aaggactgga tgcggccatt gaaaaattcg gctacccatt ggtgataaaa cccattgatg
     6181 gcaatcatgg caaaggcaat accaccaata tcaccaaccg cgaacaggcc atcaaagcct
     6241 ttgaagcggc caaacattac agccgcagcg tgatcgtgga gcgctttatc accggcttcg
     6301 atttccggtg cctggtgatc aattataaat ttatctgtgc cgccttgcgt acccccgctt
     6361 ccgtaattgg tgatggcgta catgacctgc aatggctgat cgaagaaacc aataaagatc
     6421 cgcgccgggg atatggacat gaaaaagtgc tgacgcagat caccattgac cagtttaccc
     6481 agaaaatgct ggacgatgcc ggggtgaccc tgcaacacgt tccggcaaaa ggcgaactct
     6541 ttttgttaaa acctacggcc aatctctcta ccgggggaac ctcctcggat gtaaccgatg
     6601 aagtgcaccc ggccaatgtc tttatgtttg aacggatcgc taaaatcatt ggtcttgata
     6661 tctgcgggat tgacgtaatg gcgatggacc ttcgtacccc cgtttcagaa aacggtgggg
     6721 cgatactgga agtgaatgcc gcccctggtt tccggatgca tatcgaaccc gccgaaggac
     6781 tgccccgcaa tgtggccgaa ccggtggtgg atatgctatt ccccaaagga agtgccggac
     6841 ggattcccat tattgcggtg accggcacca atggcaaaac cacgaccacc cggttgattg
     6901 cccatatcgc taaaactgcc ggaaagaaaa cgggatacac caccagtgat ggcgtgtata
     6961 tccagaacca gctgatgatg aaaggcgatt gcaccggacc gatctcttcg cagtttgtat
     7021 taaaagatcc tacagtagat tttgccgtac tggaatgtgc aaggggtggt atcctgaaag
     7081 ccggactggc ttttcaaaat tgtgaagttg ccgtggtaac caatgtagcc gcagatcata
     7141 tcgggttggg cggtattaat tccgttgaac aaatggccaa ggtaaaagcc gtagtggcag
     7201 agaccgtatt cccgcatggg tattcggtac tgaatgccga tgatgacctg gtctataaaa
     7261 tgaagaacga cctgaaatgc aatattgctc tgttcagtat ggatgaaaac aatccgcgga
     7321 tcaaggcaca ttgtgccaac catggtctgg cctgtgtata tgaaaacggc tatgtcacca
     7381 ttatgaaagg cacctggaag atccgggttt taccggcccg tgatattccc ctgacttttg
     7441 aaggaaaggc agttcataat atcaacaatt gccttccggc ggtattggcc gcttatttat
     7501 accgcgatat caatatcgac gatatccgga ccggtttgca aacctttata ccgagcgaga
     7561 gcctgactcc gggccggctg aattttttcc actttaagca tttcacgatg ctggccgatt
     7621 ttgcccataa tccacatgga ttacgcctgc tctgtgattt tatcagtaaa ctggattacc
     7681 cggccaaaat cggggtcatt agtggtaccg gcgaccgccg cgacgaggat atccgggaac
     7741 tgggagaaat ctcagctgcc tattttgatg aaattatcat ccgttgtgat aaaaaccttc
     7801 gtgggcgtac ggccgatgag attattgacc tgctgaaaga ggggattcat agtgtaaatc
     7861 cggatatccc ggtacttacg atccccaatg aggaccaggc actggactat atctatgaga
     7921 acgccaaacc gggtgcttta tacacaatta tgtgcgatgt ggtggccagg gcgctggata
     7981 agattaaaga actgaaagtg agggaggaaa aagaaagcag gcccgcctga atatttgtat
     8041 aatttcttca aattccattt gggaaaaaag gcagatagcc ctatttttgc actcccaaaa
     8101 gcaattttgg ttacggattg acccatggtg taatggtaac actactgatt ttggttcagt
     8161 catttaaggt tcgaatcctt atgggtcaac aaaaa
//
LOCUS       DAOPMW010000120         8162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9773_length_8162_cov_9.109782, whole genome shotgun sequence.
ACCESSION   DAOPMW010000120 DAOPMW010000000
VERSION     DAOPMW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9773_length_8162_cov_9.109782"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLU11_07290"
     CDS             <1..494
                     /locus_tag="PLU11_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPN58958.1"
                     /translation="MGSRLEQRYYITDLLTDILSGGGSSRLYQSLVKEQQLFSNIECH
                     HFGSTENGLVAVEGKLVKGVKIEDAEQAVMAELEKIKTVLVSELELQKVKNKTESMIA
                     FEDMSVMSRANSLAYYENLGDAAWMNDELTKYAAVTVEDIRNEAGRIFREENSNTIYY
                     LAK"
     gene            755..2032
                     /locus_tag="PLU11_07295"
     CDS             755..2032
                     /locus_tag="PLU11_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN58959.1"
                     /translation="MTINRKQAPPIVDAVNFNLQLKPYQAYTLRNGVPVYAVNAGAEE
                     VMQLEWVFFAGNSQEEQNLVAATTNFLLRNGTNTKTAFQLNEYFEYYGAYVNRACYNE
                     TATISMHCLSKHVGELLPVVKEMITQSTMPSEELAIYKQNMKQRLKVSLKKSDFVAGR
                     LIDTYLYGENHPYGKYSSAEDFDALERDQLLHFYNQYYIQGKLMLFVAGKLPENINIL
                     LDQHFGDLPNNPVEVKEHQIVLAQEKKYRITNDPNGVQGSIRMARSFPNRHHPDFLKV
                     QVLNALFGGFFGSRLMSNIREDKGYTYGIHSYLHNHIQHSAWMVSTEAGKDVAEATVA
                     EVYKEMKLLREELVDEEELLLVRNYLMGSILGDLDGPFQIIGRWKNIILNNMSENYFY
                     DAIHTIKTISAEELKGLAEKYLQPEEFYELIVV"
     gene            2409..4553
                     /locus_tag="PLU11_07300"
     CDS             2409..4553
                     /locus_tag="PLU11_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HPN58960.1"
                     /translation="MQFDRKELSDEQLVRFYRSLLWPRMIEEKMLVLLRQGKVSKWFS
                     GIGQEAISVGATLALQEDEWIMPMHRNLGVFTSRNMPLSKLFMQWQGAKEGYSKARER
                     SFHFGSREHHICGMISHLGPQLAIADGVALAHKLRKENKVSLAFTGDGATSEGDFHEA
                     LNVAAVWDLPVIFIIENNGYGLSTPVNEQYRCISLVDKAKGYGMEGIQIDGNNLLTVY
                     DTIKGVREFCIKHQKPYLIECMTFRMRGHEEASGTKYVPQDLFRLWEQKDPIKNFEDY
                     LTSIGLLNEQSIAQIREEFKHQIEEELKLGFDTKVVIADTQEELNDLYMPNSKSRESV
                     VGSRESTAAYSRSSFRGKPAGNIPTPDTSHLTASSTNPTPDTRHLSPSSTSLTANPEQ
                     LAPEKRMIDAIKEGLHQSMQRHSNLILMGQDIAEYGGAFKITEGFVEEFGKERVRNTP
                     LCESAILGSALGLSLEGYKSMMEMQFADFVTVGFNQIINNLAKIHYRWGQNADVVVRM
                     PTGAGVGAGPFHSQSNEAWFTHTPGLKVVYPSTPMDAKGLLIAAINDPNPVMYFEHKA
                     LYRSVSGPVPSEYYEIEIGKARHVRNGSELSIITYGSGVHWAEEYAAAHPEIDMDILD
                     LRTLLPLDYEAIRAAVQRTGRVLILHEDTLTGGLGGEIAAWISEHCFGMLDAPVMRCA
                     SLDTPIPFSIELERNFLAKSRLGEKVQELLQY"
     gene            4804..5340
                     /locus_tag="PLU11_07305"
     CDS             4804..5340
                     /locus_tag="PLU11_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58961.1"
                     /translation="MKKSFSLSLLLFFSLMGYCQSPNLTGEWVGVMSVDVNRTQQNVF
                     VNFSLQIKQSGQAIWGIYTGGNKLSTDSCDCAGRLTSRVSREQGVPVIFYQDGIVHHT
                     IPEDYCMYFNMLNLNYSVEDSLEILKGRWHMVLSNPTAIEGAAGVLVLAKVADKPSVD
                     VDQYFPRLQALIRRFNDN"
     gene            5520..6866
                     /locus_tag="PLU11_07310"
     CDS             5520..6866
                     /locus_tag="PLU11_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HPN58962.1"
                     /translation="MSSPTTPNLAAPVSQHERIIILDALRGMAILGILLMNIPGFGLA
                     VPSGWDPSVLNEAGTINYKLWYLVEWFPEGTQRAIFSLLYGAGIILFMKRQEEKLDGV
                     KPLEYFIRRSLWLMVFGLINAWVLLWWGDILFDYACYGMIMVVFRNLPVKSLLIGSVV
                     CFLLMVARDNRDLYLEKKVITRGEQVAAMDTTKVKLTDKQKSQLEAMNEFKDKQTHAK
                     KVERNEKSKQQVTGSYEDLYEYRTNNYLGALIRYLYMSIWDVLFFMFLGMAFLKTGIM
                     TGEASVKYYWIMAILGLGLGLLLSYYRIKPMIQYQFNEFEYMKNVKIETYTISRALRS
                     LGIFGAVMLLFKSGFFKWFFALMRPVGQMAFTNYLTQSLICGIIFYGVGFGYYGSLQR
                     YEVYLVVGAIWVLQIIWSHLWLRYFRFGPLEWCWRSLTYWKKQPMRKENTKEVLSI"
     gene            6912..>8162
                     /locus_tag="PLU11_07315"
     CDS             6912..>8162
                     /locus_tag="PLU11_07315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN58963.1"
                     /translation="MKQILAAAGMAVLLYSCGNKNQYPPEPVLYDSSYHPAVFTDTAR
                     LSRIRQYLPVIDSIYRKHAEDNHFPAISFGIVVDGELIYKNSYGYTDIAKKIPATSSS
                     MFRIASMSKSFTAMAILKLRDEGKLYLDCPAYFFVPELKNIKYPTADAPPITIRHLLT
                     HGAGFPEDNPWGDRQLADSDKDLMEFIGKQISFSNPPGIAFEYSNLGFALLGKIITEV
                     SGVPYQQYIKENIWKPLGMVNAEWEYANVPADKLAHGYRWLNNQWNEEQLLHDVPDGS
                     WGAMGSMITSIDEFAKYMALHLSSWPPSNAKETGPVNRSSVREMHHPWRWNGFNAAFT
                     YPDGRSCPVTSAYCYGLGWMKDCEGKTYISHSGGLPGFGSQWRIMPDYGIGVVGFANR
                     TYSPFGGVNLRVLDTLIKLAGLQPR"
BASE COUNT         2182 a         1761 c         2119 g         2100 t
ORIGIN      
        1 atatgggcag ccggctcgaa caacgctatt atattaccga cctgctgacg gatattctca
       61 gtggtggcgg ttcgtcccgt ctttaccagt ctttggtaaa agagcaacaa ctgttcagca
      121 atatcgagtg ccaccatttc ggcagtacgg agaacggatt agtggcggtg gaaggcaaac
      181 tggtcaaagg ggtgaagatc gaagacgcgg agcaagcagt aatggccgaa ctcgaaaaga
      241 tcaagaccgt cctggtaagt gaactggaac tgcagaaagt gaagaacaag accgaaagca
      301 tgatcgcgtt tgaagatatg agcgtaatga gccgggctaa tagtctggca tattatgaaa
      361 acctcggcga tgccgcctgg atgaatgatg aactgacgaa atacgcagca gtaacggtgg
      421 aggatatccg aaatgaggca ggaaggatat tcagggagga gaattcgaat acgatttatt
      481 atctggcaaa ataatcaccc ttcgatacgt cccgtgagac tttgatcaac aggcctgaac
      541 atttggttgg cgggactact caggatgaca cgatcattcg gctccgatta ttgaaaagca
      601 gaaatggtcc caggggtgca aaaaatacct ctacggagcc tcttaagtaa cgtgacgctc
      661 gtgtcatcct gagtagacgc ttcagcattg ctacagggca gaaacaaaat tggcagcgtc
      721 gtatcgaagg gtgacgaaac aaaaataaat tatcatgact atcaatagaa aacaagcgcc
      781 tcccatcgtg gatgccgtga attttaacct gcaactgaaa ccataccagg cctatacttt
      841 gcggaatggt gtacccgtgt atgccgtgaa tgccggggcg gaagaagtga tgcagctgga
      901 gtgggtattt tttgccggga acagccagga agagcagaac ctggtggccg ccaccacgaa
      961 ttttttgtta cgtaatggca cgaatacaaa aaccgctttt cagctgaatg aatattttga
     1021 gtattacggg gcttatgtca acagggcctg ttataatgaa acggctacca tctccatgca
     1081 ttgtcttagt aaacatgtgg gcgaactgct gccggtggtg aaggaaatga ttacccaatc
     1141 caccatgcct tccgaggagc tggccattta caagcagaat atgaagcagc ggctgaaggt
     1201 aagcctgaag aaaagtgatt ttgtagcggg gcggctgatc gacacttacc tgtacgggga
     1261 aaatcaccct tatgggaaat acagcagtgc ggaggatttt gatgcgttgg aaagagacca
     1321 gttgctgcat ttttataatc aatattatat acagggtaaa ctgatgctct ttgtggcagg
     1381 taaactaccg gagaatatca atatcctgct cgatcagcat tttggcgacc tgcccaataa
     1441 cccggtagag gtaaaagaac accagattgt cctggcacag gagaaaaaat accggatcac
     1501 gaatgatccg aatggcgtgc agggctccat ccgtatggcc cgcagcttcc cgaaccggca
     1561 tcatccggat ttcttaaaag tgcaggtgct gaatgctttg tttggcggct ttttcggatc
     1621 caggctgatg agcaatatcc gcgaagacaa aggttacacg tatggtatcc acagttacct
     1681 gcataatcat attcagcatt ccgcctggat ggtaagtacc gaagccggca aggatgtggc
     1741 cgaagccacg gtggccgaag tgtataagga aatgaaactt cttcgggaag agctggtgga
     1801 tgaagaagaa ctcttactgg tccgcaacta cctcatgggc tccattctcg gcgatctgga
     1861 tggtcccttc cagatcatcg gccgctggaa gaatattatc ctgaataata tgtcggagaa
     1921 ttatttctac gatgcgattc atacgatcaa gacgatttcg gcggaggaac tgaaggggct
     1981 ggcggagaag tatttgcaac cggaggagtt ttatgaactt attgttgttt aaggtgaggg
     2041 gcccttcggg cgctgaagag gtcaaaggag aaaaagatag agaataaaga gtgcgtgata
     2101 tgggtaaagc ctttgttaaa gcctaaataa ctaattgcta aatgcccttc gggcgctgaa
     2161 gaggtcaaag gaggaaagga gggagaataa agagtgcgtg atatgggaat gtctcatcta
     2221 aacaactgaa ttacatttgt ccttttgata ttaaagaaat agaagtataa aagtgtgttt
     2281 tttaaagcaa gagccctgta ttttccttcc ctgtctttga tattgcttat tttattagcc
     2341 tcccgcttta ctcttttctc tcgttttctc ctttgaactc ttagctaaaa attcccgaac
     2401 ttagtactat gcaattcgac cgcaaagaac tgtccgacga acagcttgtc cgtttttacc
     2461 gtagcctcct ctggccccgc atgatcgaag agaaaatgct ggtgttgctg cgccagggaa
     2521 aagtttccaa atggtttagc gggattggtc aggaagccat ttccgtaggt gccaccctgg
     2581 ccctgcagga agatgaatgg atcatgccca tgcaccggaa tctcggggtg tttacctccc
     2641 ggaatatgcc cctgagtaaa ctctttatgc aatggcaggg cgccaaagaa ggatacagca
     2701 aggcgcggga gcgcagtttc cacttcggca gccgtgaaca tcatatctgc gggatgattt
     2761 cccacctcgg tccccagctg gcgattgccg atggtgtggc gctcgcacat aaactacgca
     2821 aggaaaataa agtatccctg gcttttaccg gtgacggggc taccagtgaa ggcgattttc
     2881 atgaagccct gaacgtagcc gcggtctggg acctgccggt aatttttatc attgagaata
     2941 acgggtatgg cctgagtaca cctgtaaatg aacaataccg ttgtatcagc ctggtcgata
     3001 aagccaaagg ctacgggatg gaaggcattc agatcgatgg caataacctg ctcactgtat
     3061 acgatacaat caaaggagtg cgtgagttct gtataaaaca ccagaagcct tatctcatcg
     3121 aatgtatgac gttccggatg cggggacatg aagaagccag tggtaccaag tatgtaccgc
     3181 aggacctctt ccgtctctgg gaacaaaaag acccgatcaa aaattttgaa gattatctga
     3241 ccagtatcgg tttgctgaat gaacaatcca ttgcgcagat cagggaagaa ttcaaacatc
     3301 agattgaaga agagctgaag ctgggatttg acactaaggt tgttatagca gatacacagg
     3361 aagagttgaa tgatctgtat atgccgaact ctaagagtcg ggagtcagta gtcggtagtc
     3421 gggagtcaac agccgcatac tcccggtcat ctttcagagg aaagcctgcg ggtaacatcc
     3481 ccacacccga cacctcacac ctgacagcat cttctactaa cccgacacct gacacccggc
     3541 acctgtcacc ttcttcaacg tccctaacag ctaaccccga acagctagca cctgagaaga
     3601 gaatgattga cgccatcaaa gaaggcctcc atcaatccat gcagcggcat tcgaatctga
     3661 ttctgatggg acaggatatt gcggagtatg ggggtgcttt taagatcacg gaagggtttg
     3721 tggaagagtt tgggaaggag cgggtgcgga atactccgtt atgtgagagt gccattttgg
     3781 gttcggccct gggactttcg ctcgaaggat ataagagtat gatggaaatg cagtttgccg
     3841 attttgtgac ggtgggcttc aaccagatca ttaataacct ggcgaagatc cattatcgct
     3901 ggggacaaaa tgccgatgta gtggtgcgga tgcctacagg ggcaggcgta ggtgccggtc
     3961 cttttcatag tcagagcaat gaagcctggt ttacccatac acccggactg aaagtggtat
     4021 atcccagcac accgatggat gcaaagggat tactgatcgc cgcgatcaat gatcctaatc
     4081 cggtgatgta ctttgagcat aaagcacttt atcgttcggt gagcggaccc gtgccatccg
     4141 aatattatga aatagaaatc ggcaaagccc gtcatgtaag gaacggcagt gaactgtcca
     4201 tcattactta tggcagtggt gtacattggg ccgaagaata tgcggcagct catccggaga
     4261 ttgatatgga tatactggac ctgcggactt tattaccgct ggattatgag gctatccgtg
     4321 cggcggttca acggaccgga cgtgtactca tattgcacga agacacattg accggcggat
     4381 tgggtggcga gatcgcggcc tggatttcgg agcattgctt tggcatgctg gatgcgccgg
     4441 tgatgcgctg tgcctcgctg gatacaccca ttcctttcag tattgaatta gagaggaatt
     4501 ttctggcgaa gtcgaggttg ggagagaaag tacaagagct tttacagtat taggactggt
     4561 tggttaaaat acgtaaacca ggagtctggt tacagctatg agttacgagc tacgagtttg
     4621 attgtctaaa agagattgtc caggtaacta tgtttgatta ttcgaagttc aaaacagatg
     4681 cccactgttg gtctcgtagc tcttcgctcg ctgctttgtt ttttatgttt tctttagcag
     4741 agtatttttg tattttccca attgcaacgg ccagtgcacc cgatccccgc caaaaccccc
     4801 tgtatgaaaa agtcattttc cctttcctta ctcttatttt tttccctgat gggatattgc
     4861 caatctccca accttaccgg cgaatgggtg ggcgttatga gtgtggatgt caaccgaacc
     4921 cagcaaaatg tgtttgtcaa tttcagtttg cagatcaaac aatccggtca ggctatctgg
     4981 ggcatttata ccgggggcaa taaacttagt accgattcct gtgattgtgc cggcagattg
     5041 accagtcgtg tgagccggga acaaggggtt ccggttatct tttaccagga cggcatcgta
     5101 catcatacca taccggaaga ctattgtatg tatttcaata tgctcaatct caattatagt
     5161 gttgaagaca gtcttgaaat ccttaaaggc aggtggcata tggttttgag taacccaacg
     5221 gccattgaag gggcggccgg ggtactggta ctggcaaaag tagccgacaa accatcggtg
     5281 gatgtggatc agtatttccc caggttgcag gcgctgatca gaagatttaa tgataattaa
     5341 tagctgccgg ccgttcaacg agggaacctg ttaacttttc aaccgttcaa ctcctaaact
     5401 tttcttacct tttacatcaa tccgtttcac gggtaagtgt gggtgcgtat accttatgca
     5461 ggcaatcccg gcaaatcttc ttttcccagc cgaagcattg aaacaaaaac caaccataca
     5521 tgtccagtcc aaccaccccg aacctggcgg ccccggtcag ccagcatgaa cggattatta
     5581 tcctcgatgc cttgcgcggc atggctatcc tgggcatttt actgatgaat attccgggat
     5641 ttggtttagc ggtaccttct ggctgggacc cttcggtatt aaatgaggcc ggtacaatca
     5701 attacaaact ctggtattta gtggagtggt ttccggaagg cacccagcgg gcgatttttt
     5761 ccctcttata cggtgccggg attattttat ttatgaagag acaggaggaa aaactggatg
     5821 gggtgaaacc gctggagtat ttcattcgcc ggagtttatg gctgatggtt tttggactta
     5881 ttaatgcctg ggtgctgttg tggtggggag atatactgtt tgattatgcc tgttatggta
     5941 tgatcatggt ggtgttccgt aaccttcctg ttaaatcatt attgatcggg tctgttgtct
     6001 gttttctcct gatggtcgcg cgggataacc gggatctata cctggagaaa aaagtgatca
     6061 cccggggcga acaggtggca gcgatggata ccaccaaagt aaaactgacg gataaacaaa
     6121 aaagtcagct tgaagcgatg aacgaattca aggataaaca aacacatgcc aagaaggtgg
     6181 aacgcaatga aaagtcgaag cagcaagtga ccggcagtta tgaggacttg tatgaataca
     6241 ggaccaacaa ctacctgggc gccctgatcc gctatttgta tatgagtatc tgggatgtgt
     6301 tgttttttat gttcctgggt atggcctttc tgaaaaccgg cataatgacc ggggaggcgt
     6361 cggtaaagta ttactggatc atggcgatcc tgggactagg cctgggactg ctgctttctt
     6421 attaccggat caagccgatg atccaatatc agtttaatga gtttgagtat atgaaaaacg
     6481 tgaaaattga gacctatact atttcaagag ccctccgttc acttgggata tttggcgccg
     6541 ttatgctctt atttaaatcc ggttttttta aatggttctt cgcgctgatg cgaccagtcg
     6601 gacaaatggc gttcaccaat tacctgacac agtccctgat ctgcggaatc attttctatg
     6661 gagtgggttt tggttattat ggaagcctgc agcgttatga agtgtacctg gtggtgggag
     6721 cgatctgggt gctgcagatt atatggagtc atctctggtt aaggtatttt cgttttggtc
     6781 cgctggaatg gtgctggaga agcctgactt attggaaaaa gcaaccgatg cggaaagaga
     6841 acacaaaaga agtattatcg atataaaaga atccctgctt aatccttaat ttgagaaata
     6901 aatcagttgg tatgaaacag atcctggcag cagccggtat ggcagtgctt ttgtattcct
     6961 gcggtaataa aaatcaatat ccgccagagc cggtccttta tgattcttct tatcatccgg
     7021 cagtatttac cgacacggcc cgattatcaa ggatccgtca gtacttgccg gtgatcgata
     7081 gtatctaccg caaacatgcg gaggataatc attttcccgc gatctccttt gggatagtag
     7141 tggatggaga actgatctac aagaacagtt atggttacac cgatatcgcg aagaaaattc
     7201 cggcgacatc atcttccatg ttccgcattg catcgatgag taagagtttt accgccatgg
     7261 ctatcctgaa actccgggat gaaggcaaac tctatctcga ttgcccggct tatttctttg
     7321 tacctgaatt aaagaatatc aaatacccca cggctgatgc acccccgatc acgatccgtc
     7381 acctgctcac ccatggggcg ggcttcccgg aagataatcc ctggggggac cggcaactgg
     7441 cggatagtga taaagacctg atggagttta ttggtaaaca gatcagcttt tccaatcctc
     7501 ccggtattgc ctttgagtac agcaatcttg gatttgcctt gctgggaaaa ataattaccg
     7561 aagtatccgg tgtgccgtac cagcaatata tcaaggaaaa tatctggaag cccctgggta
     7621 tggtaaatgc cgaatgggag tatgccaatg tgcccgccga taaactggct catggttacc
     7681 ggtggctcaa caatcagtgg aatgaggagc agctgctgca cgatgtaccc gatggcagct
     7741 ggggtgccat gggcagtatg atcacgtcta ttgatgagtt tgccaaatac atggccctgc
     7801 atttatcatc ctggccgcct tccaatgcta aagaaactgg accggttaac cgcagttcag
     7861 taagagagat gcatcatccc tggcgctgga acggatttaa tgcagctttc acttatccgg
     7921 atggaaggag ttgtccggtt acatcagctt attgttatgg gcttggctgg atgaaagact
     7981 gtgaggggaa aacctatatc tcccacagtg gaggccttcc cggtttcggg agtcagtggc
     8041 gcatcatgcc ggattatggg atcggggtcg tgggctttgc caaccgtacg tattcgcctt
     8101 tcggaggagt aaaccttcgg gtattggata ccctgatcaa actggccgga ttgcagcccc
     8161 gt
//
LOCUS       DAOPMW010000121         8139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9814_length_8139_cov_6.672934, whole genome shotgun sequence.
ACCESSION   DAOPMW010000121 DAOPMW010000000
VERSION     DAOPMW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9814_length_8139_cov_6.672934"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLU11_07320"
     CDS             <1..244
                     /locus_tag="PLU11_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58964.1"
                     /translation="RFKRSGAWRLRFVLLNKQGEEIMGILPGINWKKMIHDFTLQINE
                     EFREECSSLLILHAVHCANCWMSMMNGGPVPALVSV"
     gene            269..2071
                     /locus_tag="PLU11_07325"
     CDS             269..2071
                     /locus_tag="PLU11_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN58965.1"
                     /translation="MKHTFLILLWLIVMRVSGQGNCDCSIADGKAGWSLLLQSPDKNC
                     QALGYEVYAEELVKGKKLDSAMYCLENALNFYLYSNCPPFRQAAVYKQMASIAENKAD
                     FSSALSFNLKALELAEKANNGLERASVLLNIAQVFNRLKQSDKGIEYTRQSLPLVEKM
                     NASVTKAELLNKITARYFYYFQDIQQPVFADTAEYYVRQALSVAREVQNIKEQIIALT
                     RLNAIYESRGEYKKALDFIDQALVMCVQGVHGRQLTTLYGDKGNLLRQMGDFKEARRF
                     ADSCLYYCEAEKYPPLISNAWSLIYDIENQAGNFKEALFARNKEKHINDSLSSADRAR
                     AVNELEKKYNQAKNERTIRELAQQKQIWILIGVAGLLVALVIAFFLRQQSLKHKQKIL
                     ETEQRLNRARMNPHFFFNALASLQSFAIRENDGQAIAVNLSKFSHIMRETLESTYKEY
                     VTVEQEIDFLREYLDVQKIRFPQKFSFTIEADPDLETDEVQIPAMIIQPFVENSVEHG
                     FSGIDYTGNIRVYFSKQGKELRILIEDNGKGLITGNQDKEGHVSRASQIIRDRIYLLN
                     LKLRTKASFSIDNGNGQQGVQVKIHLPLLYKEDI"
     gene            2073..2819
                     /locus_tag="PLU11_07330"
     CDS             2073..2819
                     /locus_tag="PLU11_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN58966.1"
                     /translation="MRILIIDNEPALRSGLRQLVNSIGKPEDQVEEAEGVMTGLDKIA
                     SFDPDLVFLDIEMEDGTGFDLLKQLNNPSFQLIFTTAHNKYAIQAFKFSAIDYLLKPV
                     DPGELAASMEKARTNIRKSDLNNQLAVLMQQLSAKPGPDKKIVLKDIESTYFVKISDI
                     LYCEAGGTYTKFYIDGSDPILVSKNLKEYEAILEPHGYLRTHHSYLVNPDKIKMYDKT
                     DGGALILVSGHSIPVSQRKKEMILQILENR"
     gene            3012..3743
                     /locus_tag="PLU11_07335"
     CDS             3012..3743
                     /locus_tag="PLU11_07335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPN58967.1"
                     /translation="MKRTLVIGDIHGGLRALKQVLERANVNPDDRLVFLGDLVDGWSE
                     SAQVVDYVMELALQQECIFIKGNHDEWCEDWLRTGKADSRWLLHGGMETLRSYDGYDE
                     ETKKQHLAFIAGMNYYEIDLENRLFIHAGFTSMHGPHAEFHASNFSWDRTLWEMALTM
                     DKQLTKESPYYPKRLKLFNEIFIGHTPTQYYNCEVPMNGANVWNIDTGAAFKGRLSVM
                     DIETKEYWQSDPLTSLYPGEKGRNK"
     gene            3836..5500
                     /locus_tag="PLU11_07340"
     CDS             3836..5500
                     /locus_tag="PLU11_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN58968.1"
                     /translation="MRTYLPAIVSLFFLFSCEKSTPAATGPVITGLDCSSLSFSATPT
                     AGISFNGTITVSYSGGNGKTYTAGSATLSTGVTGLTVSLQSGTLANGSGNLVFAVTGT
                     ATGPGNAGFSISFGNKSCSVILPVAENNSFEQYGTPFANVPDRKDAIIYQVNMRVFST
                     AGNFQGVIDRLDSIKAMGVNVIYLMPIYPVGVANAFNSPYCVRDYKTVNSEFGSLTDL
                     RALVDGAHSRNMSVLLDWVANHTSWDHTWISTHNDWYQKNTAGAIISPPGMGWNDVAQ
                     LDFTNTAMRLEMIRNLKYWIYTANIDGFRFDYSDGPPFSFWKQAVDSLRLINTHKLLL
                     MAEGSRSDHYSAGFDYTFGFNFYGGLRGVFGNGLPATNFDVLNNLEFSGATNGQQVVR
                     YTTNHDINGSDGTPQELYGGLTGSMSAFIAASLMKGVPMIYNGQEVGTPYRLTFPFTS
                     ADINWTLNPDLKAEYKRILAFRNNSAAIRGGTLTSFSSADVLAFTRELGTEKVLVLSN
                     VRNSSITYTVPGALSGTSWTNGITGAPVSVGTTVTLPAYSYLLLKN"
     gene            complement(5537..6241)
                     /locus_tag="PLU11_07345"
     CDS             complement(5537..6241)
                     /locus_tag="PLU11_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58969.1"
                     /translation="MFKDLHILPRITGAVLDEYLAMGWYRMGQIIFTTDYLFKEEKYY
                     RVFWLRYRMDLFRISKKQEKLLKLPPGFTVTTTPLQITEELEALYLLYSTDLDFEISP
                     TLRENLFSLCFISSEEAPAYDSWIIEIRDQGKLIAAGIFDKGEKSMMGIINLYDPAYK
                     KYSLGKRLILLKLKESVERRLDYYYPGYIVMNFPKFDYKLETGEGICEIYDTMKEKWI
                     PYSTENISNAEAPPIA"
     gene            6480..>8139
                     /locus_tag="PLU11_07350"
     CDS             6480..>8139
                     /locus_tag="PLU11_07350"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPN58970.1"
                     /translation="MRVLNLLMILFSGLIVCAQPTSYSAENEHSHNDYEQATPFWSAW
                     KQGFGSIEADIFLHQGVLLVAHEKSQLKNQFTLDSLYLAPLQKCILQNKGQVYTDSSR
                     FLQLMIDIKTEARSTLSVLAETLKKYPVLINCPTLIITISGNRPDPNDFFNWPTWIHF
                     DAVPGTIYPTTALAKVEMFSDNFKNYSSWNGKGRIPESEKTVLQSLIEKTHQAGKKIR
                     FWNAPDGLNSWYAFTSMGVDYINTDQVEGLGTFFRQLPDRFHEQKQGYDLYQPQWRND
                     GLDKPVKNIILLIGDGTGLPQWYSGYTANRGKLNVFNMKSTGYSKTSSYDNYITDSAP
                     GATAFSTGQKTNNRAVGVDHTGKKLRLIPDIIRSRGMKTGIITSGDLRDATPAAFYAH
                     QSERSNYKEILNDLLLAPVDIIMGACELPAGDSILLALKKKFTVKSSVDSLTGTGAPL
                     FVADMRAALSVQNGRGNWALQAFRYSTEQFEKNKAGFFLMLEGAQIDHGGHANKLPYV
                     VTELLDFDLVIGEAMRFADSNGETLVIVTGDHETGGLTLTDGDYT"
BASE COUNT         2306 a         1686 c         1943 g         2204 t
ORIGIN      
        1 ccgctttaag cgttccggtg catggcgcct gcgctttgtc ttactgaaca agcagggtga
       61 agagataatg ggtatacttc ccggaattaa ctggaagaaa atgatccatg attttaccct
      121 gcaaattaat gaagagttca gggaagagtg ttcatcttta cttattttac atgcggttca
      181 ttgcgccaac tgctggatga gtatgatgaa tggcggccct gttccggcat tggtaagcgt
      241 ataatcttcc ttaaaacgaa aggacagaat gaagcatact tttttaatac tgctgtggtt
      301 aatcgtgatg cgggtttccg gccagggcaa ctgtgattgt tccattgcgg atgggaaagc
      361 gggctggtcc ctgctccttc aatcaccgga taagaactgt caggcgcttg gctatgaagt
      421 atatgccgaa gaactggtaa aagggaaaaa gctggattcg gccatgtatt gcctggaaaa
      481 tgcattgaat ttttacctct acagtaactg tcccccgttt cgtcaggcgg ctgtatataa
      541 gcagatggct tccattgctg agaataaggc cgatttttcg tcggcgctct catttaacct
      601 gaaagcactt gaattggcgg aaaaggcgaa taatggattg gaacgggctt cagttttact
      661 gaatattgct caggtgttta accggcttaa gcagtctgac aagggaattg aatataccag
      721 gcaatcattg ccgttggtgg agaaaatgaa tgcatctgtc accaaagcag aattgctgaa
      781 taagattacg gcccggtatt tttattattt ccaggatatt cagcaacctg tatttgccga
      841 caccgcggaa tattatgtgc ggcaggcact ttctgtggct agagaggtgc aaaatataaa
      901 agagcagatt attgccctga cccgtttaaa tgcgatttat gaaagcaggg gggaatataa
      961 aaaagcgctg gactttattg accaggcgct tgtaatgtgt gtacaggggg tccatggccg
     1021 tcagctaacc accctgtatg gtgataaagg taacttactg cgtcagatgg gtgattttaa
     1081 agaagcccgc cggtttgcgg actcctgcct gtattattgt gaggccgaaa aatatccccc
     1141 gcttatttcc aatgcgtgga gcctgatcta tgatatcgaa aatcaagccg gtaattttaa
     1201 agaagcactt tttgcaagga ataaggagaa gcatattaat gacagtctca gttctgctga
     1261 ccgtgcccgg gccgtgaatg aactggaaaa aaagtataac caggcaaaaa atgagcgaac
     1321 cattcgtgaa ctcgcccaac aaaaacaaat atggatactg atcggggtgg ccggattgct
     1381 ggtggccctt gttattgctt ttttccttcg acagcagtca ttaaaacaca aacagaaaat
     1441 tcttgaaacc gagcaacggc tgaaccgggc ccggatgaat ccacattttt tctttaacgc
     1501 gctggcctct ctgcaaagct ttgccatccg ggagaatgac ggacaggcta ttgcggttaa
     1561 tctttccaag ttcagtcata tcatgcggga aacactcgag agcacttata aggaatatgt
     1621 aacggtggaa caggagattg attttctccg ggaatatctc gatgttcaaa agatccggtt
     1681 tccgcagaaa ttcagtttta caatagaggc tgatccggat ctggaaacag atgaagtaca
     1741 gattccggcg atgatcattc agccatttgt agaaaacagc gtagagcatg gattcagcgg
     1801 catagattat acgggtaata tccgggtata tttcagtaag caaggaaagg aattgcggat
     1861 tctgattgaa gataatggca aagggttgat taccggaaac caggataaag aaggtcatgt
     1921 aagccgggcc agccagatca tccgggaccg gatttacctg ctgaacttaa agctccggac
     1981 gaaagccagt tttagtattg ataacggtaa cggtcagcaa ggggtacagg taaagattca
     2041 cctgccttta ctatataaag aagatatata atatgcgcat actcatcatc gataatgaac
     2101 cagcattgcg gagcgggctc cgtcagctgg tgaattccat aggtaagccg gaagatcagg
     2161 tagaggaggc agaaggggtt atgacaggac tggataaaat agccagcttt gatccggatc
     2221 ttgttttcct ggatattgaa atggaagacg gtacgggatt tgacctgtta aagcagttga
     2281 ataacccgtc ctttcagctg atttttacca cggcacataa taagtacgcg attcaggcat
     2341 ttaagttcag cgcgattgat tatttactca aaccggtaga tccgggcgaa ctggcggcga
     2401 gtatggaaaa ggccaggacc aatatccgta aatcggacct gaataaccag ctggccgtgc
     2461 tgatgcagca gttatcggcc aaaccaggcc ctgataaaaa gattgtacta aaggatattg
     2521 aatccactta tttcgtaaaa atcagtgata tcctgtattg tgaagcgggc gggacgtata
     2581 caaaattcta tattgatggt tcagacccga tcctggtctc caagaacctg aaagaatatg
     2641 aagccatact tgaaccgcat gggtatttac gcacccacca ttcatacctg gtgaatcccg
     2701 ataagatcaa aatgtatgat aaaaccgacg gcggggcatt gatcctggtc agcgggcact
     2761 ccattccggt cagtcagcgg aaaaaggaga tgatcttgca gattctggag aaccggtaat
     2821 tagttgcatg aatcttaccc tgaagcactc agtaaatttt ttactcttgt tcaccggcat
     2881 atatcggaaa taatcagact gattttgact tttagaatag gtagaggtgg ttttttcaaa
     2941 aggcgctgga attttaatat taagtactaa ggctgactga tacaatccat cccattgaaa
     3001 tatattgcag tatgaaaaga acattggtca ttggcgacat acatggcggc ttaagggctt
     3061 tgaagcaggt tttggaaaga gccaatgtga accccgatga ccggctggta ttcctgggcg
     3121 atctggtgga tggctggagt gaatcggccc aggtggtgga ttatgtgatg gaactggcac
     3181 tacaacagga atgtattttc atcaaaggaa atcatgatga atggtgtgag gactggttgc
     3241 gtacaggaaa ggcagacagc cgctggctcc tccatggcgg catggaaaca ctccgcagtt
     3301 atgacggata cgatgaggaa acaaagaagc agcacctggc ctttattgca gggatgaact
     3361 attatgaaat cgacctggaa aaccggctct ttatccatgc tggttttacc tccatgcatg
     3421 gtccgcatgc agagtttcat gcgtctaatt tcagctggga ccggaccctt tgggaaatgg
     3481 cgctgaccat ggataaacaa ttaacaaaag aatcgccgta ttatcctaaa cgattaaaat
     3541 tattcaacga aatttttatc gggcatacac ccactcaata ttacaattgt gaagtgccaa
     3601 tgaacggagc gaatgtctgg aatattgata caggggctgc ttttaaaggc cggttatctg
     3661 tcatggatat tgaaacaaaa gaatactggc aaagtgaccc actcaccagc ctttatccgg
     3721 gtgaaaaggg aagaaataag taagtcccgg gtaagatcag gtcagtctgt gatttagatt
     3781 gtgatacctt gcaccatatt tgaagtagta ttgtgctgaa ggttaaacta ttattatgcg
     3841 tacttatctg cctgctatag ttagtctctt ttttttgttt tcctgtgaaa aatcaactcc
     3901 ggcagcaacc ggaccagtca ttaccggatt ggattgttct tccctgagtt ttagcgcaac
     3961 acccacggct gggatttctt ttaatggtac gatcaccgtc agttattccg gtggaaacgg
     4021 aaaaacgtat acagctggat ctgcaaccct ttctacggga gtaaccggat taacagtcag
     4081 tcttcagtca ggtacactgg ccaatggctc cggaaacctg gtttttgcag taacaggtac
     4141 agcaactggt ccgggaaatg ccggattcag catcagtttt ggaaacaaaa gctgttctgt
     4201 tattttaccg gttgccgaga ataacagctt tgaacagtat ggtactcctt ttgccaacgt
     4261 accggaccgg aaggatgcca ttatttacca ggtaaacatg cgggtgttca gcacagccgg
     4321 aaatttccag ggcgtgattg atcgcctgga ttcaatcaaa gccatgggtg ttaatgtcat
     4381 ttacctgatg cccatttacc cggttggtgt ggccaatgcg tttaattcac cctattgcgt
     4441 gagagactac aagacggtta attcagaatt cggatcgctg acagatctcc gggcgctggt
     4501 ggatggagca cacagccgca atatgagtgt gttgctggat tgggtggcca atcatacttc
     4561 ctgggatcat acctggatca gcacacataa tgactggtat cagaaaaata cagcaggcgc
     4621 catcattagt ccgccaggca tgggctggaa tgatgtggcc cagctggact tcactaatac
     4681 cgcgatgcgg cttgaaatga tccgtaacct gaagtactgg atttacacgg ccaatattga
     4741 cggattccgc tttgattatt cagatggccc acctttttca ttctggaaac aagcggtgga
     4801 ttccttacgc ctgatcaata cccataaatt attattaatg gcggaaggca gccgttcgga
     4861 tcattattcc gccggtttcg attatacatt tgggttcaat ttttatggtg gactacgggg
     4921 ggtatttgga aatggactac cagccaccaa ctttgatgtc cttaataacc tggagttttc
     4981 aggagccacg aacggacagc aggttgttcg ttataccacc aatcacgata tcaatggatc
     5041 cgatggtacg ccgcaggagt tatatggagg tttaaccggc tccatgtccg cctttattgc
     5101 ggcttcattg atgaaagggg tgccgatgat ttacaatgga caggaagtcg gcacgcctta
     5161 ccggctgacc tttccattta ccagtgcgga tatcaactgg acactgaacc cggatttaaa
     5221 agccgagtat aaacggatac tcgcttttcg taacaacagt gctgccattc ggggtggtac
     5281 actgacttct ttcagcagtg ccgatgtact ggcttttacg agggaactgg ggacagaaaa
     5341 agtattggtt ttatcgaatg tgagaaacag cagtattacg tatactgttc cgggggcact
     5401 ttcaggaacg agttggacaa atggaattac cggtgcaccg gtaagtgttg gaactactgt
     5461 tactttacct gcctattcct acctgctgct aaaaaattaa agaaataaaa aaacaggtca
     5521 acctgcatct tcggctttat gcgatgggag gagcttccgc gttgctgata ttttccgttg
     5581 agtacggaat ccatttttct ttcatggtat cataaatctc acatatccct tcacctgtct
     5641 caagtttgta atcaaacttg ggaaaattca tcacaatata acccgggtaa taatagtcca
     5701 gccggcgttc cacagactct ttgagtttga gcaaaatcag ccgtttaccc agactgtatt
     5761 ttttataggc cgggtcatac aggtttataa tgcccatcat acttttttct cccttgtcaa
     5821 atatccctgc agcgatcagt ttaccctgat cacggatttc aatgatccaa ctgtcataag
     5881 caggggcttc ttccgaactg ataaagcaaa gggagaacag gttttcacgc agcgtaggac
     5941 tgatttcaaa atccagatca gtgctatata gcagataaag tgcttccaat tcttcagtaa
     6001 tctgtagcgg ggtagtggtc acggtgaagc ctggtggcag tttcaggagt ttttcctgtt
     6061 ttttagaaat ccggaaaagg tccatccggt agcgcaacca gaatacccgg taatattttt
     6121 cttctttaaa cagataatcc gtggtgaata taatctgccc catccggtac cagcccatgg
     6181 ccaggtactc atccagtact gcaccagtta tgcgggggag tatatggagg tctttgaaca
     6241 ttgtttgtaa acaaagaaac aaaaatattc gcaggttata ccagccaaat aatcatcagc
     6301 taacgtttca ataatataac cttaacctca tttttctttc tccctcaatt cattgatata
     6361 tgtttgttat ccggattccg gataccggtc atttcctgcc gccaaatcga taaattgcat
     6421 tttatagaag ggttggttta ctgatagtag acggcttcta ttaatataaa accacattta
     6481 tgcgggttct taacttatta atgatcttgt tttccggttt gattgtttgc gctcagccta
     6541 cttcttattc tgctgaaaat gaacattcac ataatgacta tgaacaggca actcctttct
     6601 ggtctgcctg gaaacagggc tttggttcaa tcgaagctga tattttcctg catcagggtg
     6661 ttttacttgt ggcccatgaa aaaagccagc tgaaaaacca gtttacactg gattcacttt
     6721 acctggcacc cttgcaaaaa tgtatcctgc aaaataaagg gcaggtatat acagacagca
     6781 gccgttttct tcagctcatg atagatatta aaacagaagc ccggtcaaca ctgtcggtat
     6841 tagctgaaac gctcaaaaaa tatccggtcc tcatcaactg tccaacactc attatcacca
     6901 tctccggcaa ccggccagat cccaatgatt tttttaattg gcctacctgg attcattttg
     6961 atgcggtgcc tggaacaatc tacccgacca cggcattagc aaaagtggaa atgttcagtg
     7021 ataattttaa aaactattct tcctggaatg ggaagggtag aattcccgaa tcggaaaaaa
     7081 ccgttctcca gtctcttatt gagaagactc atcaggcagg gaaaaaaatc cgtttctgga
     7141 acgcaccgga tggactcaac agctggtacg catttacgtc catgggtgtt gattacatca
     7201 atacagacca ggtggagggg cttggtactt ttttcaggca gttgccggac cggtttcatg
     7261 agcagaaaca gggatatgat ttgtatcagc cacaatggag aaatgacgga cttgataaac
     7321 cggttaaaaa tattattctg ctgattggag acggcacagg tttgccccaa tggtacagtg
     7381 gatacaccgc gaatcgtgga aagctcaatg tttttaatat gaaaagcacg ggctattcca
     7441 aaaccagttc ttacgataat tatataactg attccgctcc cggagctact gctttttcaa
     7501 ccggccagaa aaccaacaac cgggctgtag gggttgatca taccgggaaa aaactacgat
     7561 tgattcccga tatcatccgg agcaggggca tgaagacagg gattatcacc agtggtgatt
     7621 tgagggacgc taccccggca gcattttatg cccaccagtc agaacgcagc aattataagg
     7681 agattctaaa tgatttacta ttggcgccgg tggatatcat tatgggagct tgtgaacttc
     7741 cggcgggaga tagcatactg ttggcgttaa agaaaaaatt taccgttaaa agttcagtag
     7801 atagtttgac tggaacaggg gctcccttgt ttgtagctga tatgagagcc gcactttctg
     7861 ttcaaaatgg aaggggtaac tgggcactac aggcttttcg atattcaaca gaacaattcg
     7921 aaaaaaataa agcgggtttc tttctgatgc tggaaggggc acagattgac catggcggac
     7981 atgcgaataa actgccgtat gtcgtaactg agttactgga ttttgatctg gttattggag
     8041 aagccatgcg ttttgccgat agcaatgggg aaaccctggt gattgttacc ggagatcacg
     8101 aaaccggcgg actaacacta actgatggtg attatacaa
//
LOCUS       DAOPMW010000122         8073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9928_length_8073_cov_13.139187, whole genome shotgun sequence.
ACCESSION   DAOPMW010000122 DAOPMW010000000
VERSION     DAOPMW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_8073_cov_13.139187"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLU11_07355"
     CDS             <1..219
                     /locus_tag="PLU11_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58971.1"
                     /translation="YIKGNKCKKFESDAACIAYYKTWQDKRYDKWRAKHPKADYYHFL
                     KHVKYATGDKYTNELKPKVAWVKKNLKL"
     gene            complement(304..1590)
                     /locus_tag="PLU11_07360"
     CDS             complement(304..1590)
                     /locus_tag="PLU11_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HPN58972.1"
                     /translation="MSEKTNRTIQVINVALFCSVLLIGGVISLSMKKKDISETENRKL
                     AAFPVYSDSSLWSGDYFRGIEMYYADNFPYRDKWIDVSSSFRNKLGFESTDIKIYAPQ
                     NNAEANEKEEIDTTKNQTAGLRPDDGAVGEVKKSLFVFKNRAFEMFGGGPGMGKAYAD
                     VINSYNRAGIPNLQVYNLIIPVALEFELTEKYKKLQKPNRPAIEHVYNSLDSNIKKVW
                     AIDEMRKHREEYIYFNTDHHWTSLGAYYAYRAFCESAGLTPVSLDTVRSKVKPAFLGS
                     LYRLTRDPKLKENPDSVRYYIFKDSLRFYIGSVTSLNYWGKSKMYGEGASGSNSYSVF
                     LQGDLPIVKMETQHKNGRKIAIVKESYGNAFAPFLINNYEKVIVVDQRYYTGDFMGML
                     KAEGINELLFINNIFAAHTPFHIQKIKGLRQVKNQK"
     gene            complement(1594..3021)
                     /locus_tag="PLU11_07365"
     CDS             complement(1594..3021)
                     /locus_tag="PLU11_07365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPN58973.1"
                     /translation="MVFASLIFLFLFLPLNLLLYYSTKNATWRNWVLIVFSLAFYGWG
                     EPIWISLLILSSMVDWANAIFIDKHRGTKWAKIGVTFTVVLNLCLLATFKYDQFLVDQ
                     VNALLGTSFTAPGYALPLGISFYTFHTISYVVDVYRGDIKAQRNFPNFLMYVSLYSSL
                     VAGPIIRYAHVATEIDSRKENLRDFSLGISRFCIGLFKKVVIANVAAELVLKYMGDKG
                     SPLTMEGLNHLSTPEAWVGLIMFAIQIYFDFSGYSDMAIGLGRMFGFHFRENFEYPYI
                     SKSISEFWRRWHISLGSIFRDYVYIPLGGNKKHVYFNLLVVWFLTGMWHGASWNFIFW
                     GLYFFIFIALEKAFLQKWLDKAPAFIAHFYALAIIIPGWVIFYFTDTTMLFAYIKKLF
                     TWSPATDGYLDLKNTMTLHLFWLIMAIAFCMPVYHKVRAWVEKRSGRITLYDSVTIGF
                     NMALLFICVAQLVGKSYNPFIYFRF"
     gene            3180..3530
                     /locus_tag="PLU11_07370"
     CDS             3180..3530
                     /locus_tag="PLU11_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPN58974.1"
                     /translation="MQLTAKLIQLLPLQTGNGKNGQWKKQDIIVETEGTYPKKVCISI
                     WGDKISESLLKPGSMLNISFDVESREYNGRWYTDVKAWKVEPAGAAGTVDRIPDDNVH
                     FEEGQMEGGGDLPF"
     gene            complement(3687..5234)
                     /gene="rpoN"
                     /locus_tag="PLU11_07375"
     CDS             complement(3687..5234)
                     /gene="rpoN"
                     /locus_tag="PLU11_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPN58975.1"
                     /translation="MVQKVQAVCRNPDQAAPAKNTMALSQSLQHKLLQKLSPQQIQLM
                     KLLQVPTANLETRIKEELEENPALELDEEGHDENADDLKDEFSEEEEYDDMDGSEDEY
                     ENIDVSEYVQDGDDEVGDYKLRDDNYPEEDESRQLPHKNEASFYETLIDQLGLLNLEG
                     KQQAIAEQIVGSIDEDGYLRRETSAIVDDLAFRQNIIASEEEVEAIIRRIQHFDPPGV
                     AARDLRECLLIQLMRLKEEGKPMDLAIRAISKYFDEFTKKHYEKIQRGLNIDDDQLRE
                     VMQQIIRLSPKPGGNVGEINKAESYVVPDFFILNNAGTLELTLNSRNAPELRISEGYR
                     DMLKDYERGSKKDKRQKEAVLFIKQKIDSAKWFIDAIKQRQHTLLSTMDAIMQHQYEF
                     FLTGDETELRPMILKDIAEKTFLDISTVSRVANSKFVQTEFGTYRLKFFFSESLSTES
                     GEEVSTREVKKILSDFIEGEDKKRPLSDERLTELLQEKGYNIARRTVAKYREQLNIPV
                     ARLRKEL"
     gene            5346..5765
                     /locus_tag="PLU11_07380"
     CDS             5346..5765
                     /locus_tag="PLU11_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58976.1"
                     /translation="MHKNNAVKLNVKKTNMVQRVSGFLVVFLLMFFGSMNGQQSPVPG
                     SDSFGLKMPHYQTNVKPFQSRFSKNTRKHVPLSFTATRINLLNLPDASNYVRNLGFFC
                     KQELRLDKVTGVPVRFRLGSKEYVDHLEYGARYLPTR"
     gene            complement(6059..7279)
                     /locus_tag="PLU11_07385"
     CDS             complement(6059..7279)
                     /locus_tag="PLU11_07385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPN58977.1"
                     /translation="MPGYELWSDKERKEVNDVLETGILMRYGFDGPRKGIWKSKELEA
                     AICETFGSKYAQLTSSGTSALTTAMSALGIGYGDEVITPAFTFVASFEAVLSVGAVPV
                     LVDVDDTLTLDPAAVRKAITPKTKAIMPVHMCGSMADLDALQAICKEHNLILIEDACQ
                     SIGGSYKGKMLGSIGDAGTFSFDFVKTMTCAEGGVVMTNREDIYVKSDGYTDHGHDHL
                     GVDRGADLHPFIGYNYRISELHAAVGLAQIRRLSEFLTIQKKNNAQLKAILATVPEIS
                     FRRIPDPAGDSCSFISWFLPTEEQTRAVVAELKAQGILAGNFYWFDNNWHYIRKWDHL
                     KNAITLNALSPELKTRVMEQANKDFAASHAVMNRCISTAISLTWTEEQIKEKGEKMVA
                     VVREVVSRESGVRS"
     gene            7512..>8073
                     /locus_tag="PLU11_07390"
     CDS             7512..>8073
                     /locus_tag="PLU11_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPN58978.1"
                     /translation="MLYFLPAEAQWRLGFRAGLNGSNLVHRSDRHDKRTTGAVVSRLN
                     GGLLLEIPLEDQWYINTGIHYSGKGANMFASDSTMKKDSERVRLNYLEIPVMVVYKFP
                     SEKENQFILSSGPYIGYGFNGTINWKGGFIPTKDQVHRKEEEEYRRFELGWMISAIYE
                     IKSRYGLRLDFSKSLLNIQHPETRQKN"
BASE COUNT         2115 a         1887 c         1896 g         2175 t
ORIGIN      
        1 tatataaaag gaaacaagtg caaaaaattt gaatccgatg cggcctgcat cgcttattac
       61 aaaacctggc aggacaaacg ctatgataaa tggcgggcca aacatcctaa agccgattac
      121 tatcacttct taaaacatgt gaaatacgcc accggggata aatacacaaa tgaattgaaa
      181 ccgaaagtgg cgtgggtgaa gaagaatctg aagctgtaaa taattcaaaa gtcaaaagta
      241 aaaattaaaa acagcgtatt gactttaaag agtcttacag gtttcggagt acctttttac
      301 tttttacttt tgatttttga cttgccttaa tccttttatc ttctgtatat gaaacggcgt
      361 atgcgccgcg aagatgttgt tgatgaataa aagttcattg atgccttcgg ctttgagcat
      421 gcccatgaaa tcgccggtgt aataacgctg atccaccaca atcactttct cgtaattgtt
      481 tattaagaaa ggagcaaagg catttccgta tgactccttc acgatcgcga tcttcctgcc
      541 atttttatgc tgggtttcca ttttaactat gggcaggtca ccctgcagga atacggaata
      601 gctattgctt ccactcgccc cttccccata catttttgat ttgccccagt aattcagact
      661 cgtcacactg ccgatataaa aacgtaagga atccttaaat atataatacc gcaccgaatc
      721 cgggttctct ttcagtttgg gatcacgggt caggcgataa agcgaaccca ggaaggcggg
      781 ttttactttg gaacggacag tatccagtga tacgggagtc agtccagctg attcacagaa
      841 agcccgatag gcataataag cacccaggct ggtccagtgg tggtcggtat tgaaatagat
      901 atattcttcc cggtgtttgc gcatttcatc aatggcccag acttttttaa tattcgaatc
      961 cagggaatta tatacgtgct caatggccgg acgattcggc ttctgcagtt tcttgtattt
     1021 ttcggtcagt tcaaactcga gcgccaccgg aatgatcagg ttatagacct ggaggttggg
     1081 aataccggcg cggttatatg aattgatgac atccgcatag gctttaccca tacctggtcc
     1141 acccccaaac atttcaaaag cacggttttt aaatacaaac agacttttct tcacctcacc
     1201 tactgcgcca tcatccggac gaagaccggc tgtctggttt ttagtggtat caatttcctc
     1261 tttctcgttg gcttccgcat tattttgcgg ggcatagatt ttaatatcag tggattcgaa
     1321 tccgagtttg ttgcggaagg aggaggatac atcgatccat ttatcccggt acggaaaatt
     1381 atccgcataa tacatttcaa taccccggaa atagtccccg ctccagagtg aactgtcgga
     1441 gtagaccggg aaggctgcca gtttccggtt ttcggtttct gaaatatcct ttttcttcat
     1501 cgacagggaa atgacaccgc cgatcagcag tacactgcag aaaagcgcga cattgattac
     1561 ctggatcgtt ctgtttgttt tttcactcat gggttaaaaa cggaaataaa taaacggatt
     1621 ataactttta cccaccaact gggccacaca aataaacaac agcgccatat tgaacccgat
     1681 cgtcaccgaa tcatacaagg taatccggcc ggatcttttc tccacccagg cccttacttt
     1741 atgatagacc ggcatacaga aagcaatcgc catgatcagc cagaacaggt gcagggtcat
     1801 ggtattcttc agatcgagat agccatctgt cgccggcgac caggtaaata attttttgat
     1861 ataagcaaac agcatggtgg tgtcggtgaa ataaaagatc acccagcccg gaataatgat
     1921 cgccagcgca tagaagtggg cgataaaagc cggggcctta tccagccatt tctgcaaaaa
     1981 cgctttttcc agggcaataa atataaaaaa gtacaatccc cagaaaataa aattccagct
     2041 ggctccatgc cacataccgg tgaggaacca gacgacgagc agattaaaat acacgtgctt
     2101 cttgtttccg ccaaggggga tatatacata atcccggaag atgctgccca gcgaaatatg
     2161 ccagcgacgc cagaactcac tgatggattt ggaaatataa ggatactcaa agttctcccg
     2221 gaagtgaaag ccaaacatcc ggccgagtcc gatggccata tctgaataac cggaaaaatc
     2281 aaaatagatc tggatggcaa acatgatcag ccccacccag gcttccggcg tggagagatg
     2341 gttcagtccc tccatcgtga gcggactgcc tttgtcgccc atgtatttta aaacaagttc
     2401 ggctgccaca ttcgcgatca cgactttctt aaataatccg atgcagaaac gggagatgcc
     2461 gagactgaag tccctgaggt tttctttccg gctgtcgatc tcggtagcca catgcgcata
     2521 acgaatgatg ggcccggcta ccagggagga atagagggag acatacatca ggaagttggg
     2581 gaaattgcgt tgggccttga tatccccgcg gtatacgtcc accacataag aaatagtatg
     2641 gaaggtataa aaggatatcc ccagcggcag ggcatatccc ggtgcggtga aggaagtacc
     2701 cagcagcgca tttacctggt ccaccaggaa ttgatcatat ttgaaagtag ccagcaggca
     2761 gagattcagg actaccgtga atgtcacccc gatcttggcc catttggtgc cccggtgctt
     2821 gtcgatgaaa atggcattgg cccaatccac catggaggaa aggatcagta aggaaatcca
     2881 gatcggttct ccccaaccat aaaaagccag tgaaaagacg attaataccc agtttcgcca
     2941 ggtcgcgttt ttcgtactgt aatacaacag caggttcagc ggcaggaaaa gaaacaggaa
     3001 aataaggctt gcaaaaacca ttgttcagtg ggggttaggg aatattagcc gtgtaaaata
     3061 catttttgcc ttttgttttt atagtgaata gcctgctttt ttataaaaaa tacccttgaa
     3121 agggtatctg cctgctttta attgacagtt gcttaacttc acggccgaaa taaacacaca
     3181 tgcagcttac cgccaaactc attcaattgc tccctttgca gaccggtaat ggtaaaaacg
     3241 gtcaatggaa aaagcaggat attatagttg aaaccgaggg tacctacccg aagaaagtat
     3301 gtatctccat ctggggagat aaaatcagtg aatcactgct taaaccgggc tccatgctca
     3361 atatctcctt tgatgtggaa agccgtgaat acaatggccg ctggtacacg gatgtcaaag
     3421 cctggaaagt agaacccgcc ggtgctgcag gtacggttga ccgtattccg gacgataatg
     3481 tccattttga agaaggtcag atggaggggg gaggggactt gccattttaa gaagttgaca
     3541 ggttaacaga cctgcctgcc ggcaggcagg ttgataagtt gacaaggggc ctcctaagga
     3601 ttgtttgctg taaagaacgg aatgccgttc cttattaact tgtcaacctt tcaacttttc
     3661 aactctctca actctcacac atcaacttac aactctttcc ttaaccttgc taccggtata
     3721 ttcaactgct cccggtactt cgccactgta cggcgggcga tattgtagcc tttttcctgg
     3781 agaagttcgg tcaggcgctc atcactcagc gggcgctttt tatcttcccc ttcaataaag
     3841 tcactcagaa tttttttcac ttcacgggtg gagacttcct cgccgctttc tgtacttaag
     3901 gattcactga agaagaattt gaggcggtag gttccgaatt cggtttgtac aaatttgctg
     3961 ttggccaccc ggctcacggt ggaaatatcg aggaaggtct tctcggcaat atctttcagg
     4021 atcatggggc ggagttcggt ctcatccccg gtaaggaaga actcatactg gtgttgcatg
     4081 atggcatcca tcgtgctcag cagggtatgc tgccgttgtt tgatggcatc aataaaccat
     4141 ttggccgagt caatcttctg tttgataaag agtacggctt ccttctggcg tttatctttt
     4201 ttcgatcccc gctcatagtc tttcaacata tcccggtagc cttcgctgat ccggagttcg
     4261 ggtgcattcc ggctgttgag ggtcagttcc agggtgccgg cattattgag aataaagaaa
     4321 tcgggcacta catagctctc tgctttattg atctcaccca cattgccgcc gggtttggga
     4381 ctcaggcgga tgatctgttg catcacttcc cggagctggt cgtcatcaat attcaacccg
     4441 cgctggatct tttcatagtg cttcttggta aactcgtcga agtattttga aatggcccgg
     4501 atagccaggt ccatcggttt accctcttct ttcaggcgca tcagctggat cagtaaacat
     4561 tcccgaaggt cacgggctgc cacaccgggc ggatcaaaat gctggatccg gcggataatg
     4621 gcttccactt cttcttcgga ggcaatgata ttctgacgga aggccaggtc atccacaatg
     4681 gcggaggttt cccggcgcag gtagccgtct tcatctatac tgcccacgat ctgttcagca
     4741 atggcctgtt gtttgccttc gaggttcagc aatcctaact ggtcgatcag ggtttcgtag
     4801 aaactggctt cattcttatg gggcaactgc cggctctcat cttcttcggg gtagttatca
     4861 tcgcggagtt tgtaatcgcc tacttcatca tctccgtcct gcacatattc gctgacgtcg
     4921 atattttcat attcatcctc gctgccgtcc atgtcatcat attcttcctc ttcactaaac
     4981 tcatctttca ggtcatccgc gttttcatca tgcccttctt catccagttc cagggccgga
     5041 ttttcttcca gttcctcctt gatccgggtt tccaggttgg ccgtaggtac ctgcagcagt
     5101 ttcattaact gaatctgctg cggggataac ttctgcagta acttatgctg taaactctga
     5161 ctcagggcca tggtgttctt cgccggagct gcctggtccg ggttccggca aaccgcctga
     5221 accttttgaa ccaatgactc cttttttggt taacttgtat gctacttatt ttaccggggt
     5281 ctcccacagg agacaccgat gaatttaggg tctcttacag gggacccagg taaaaaattc
     5341 cgcctttgca caaaaataat gccgtaaaat taaatgtaaa gaaaacaaat atggtgcaac
     5401 gagttagcgg gtttcttgtt gtgttcctgt taatgttttt tggaagcatg aacgggcaac
     5461 aatcgcctgt gcccggatca gacagttttg gattaaaaat gcctcattat caaacgaatg
     5521 tgaagccatt ccagtcacgg ttttctaaaa acacccggaa gcatgtcccc ttgagtttta
     5581 cagctacccg tattaatttg ttaaaccttc ccgacgcctc caattatgtc cggaatctcg
     5641 gttttttctg taaacaggag cttcggctgg ataaagtgac gggtgtaccg gtaaggttcc
     5701 ggctggggtc gaaggagtat gtggatcacc tggagtatgg tgcccgatac ctgccaaccc
     5761 ggtagaacgt cttttttatt ggaggcgata ataaatacag aacaacattt cccgcagatg
     5821 gcgcagaata catttcccgc agatggcgca gaatacattt cccgcagatg gcgcagattg
     5881 aatacagcgc agatttacgc agatttgttt ctggataatt agaataaatc tttgaaaaca
     5941 ataaatacgg attcctgaaa attgtaagta aagaaggtgc tgcgttaatc tgcgcttcaa
     6001 tccgcggaaa tctgcgggag attaatgttg tgctttttgt ttttcagttg ctttaactct
     6061 aactcctaac tcctgactcc cgactaacga cttccctcac taccgccacc atcttttctc
     6121 ccttctcttt gatctgttct tccgtccagg ttaaactgat cgccgtagag atacagcggt
     6181 tcatcaccgc atgactggcc gcgaaatctt tgttggcctg ttccattacc cgggtcttca
     6241 gttcgggact gagggcgttg agggtgatgg catttttcag gtgatcccat ttgcggatat
     6301 agtgccagtt attatcaaac cagtagaaat taccggcgag gatgccctgt gctttcaatt
     6361 ctgctaccac ggcccgggtc tgttcttcgg tgggcaggaa ccaactgata aaactgcaac
     6421 tgtcaccggc cggatcgggg atgcggcgga aactgatctc cggtacggta gcaagtatgg
     6481 cctttaattg agcgttattc tttttttgga tcgtaagaaa ttcactgagc cttctgatct
     6541 gcgccagtcc gactgccgca tgtaattcag aaatgcggta attgtaaccg ataaatggat
     6601 gcaggtccgc cccgcgatcc accccgaggt gatcatgacc gtgatcggta tagccatcgc
     6661 tttttacata aatgtcttcg cggttggtca tcaccacccc gccttcggca caggtcatgg
     6721 tttttacaaa gtcaaaggag aaagtaccag catccccgat actgccgagc atttttcctt
     6781 tataggaacc accgatactc tggcaggcat cttcaatcag gatcaggtta tgttctttac
     6841 aaatggcctg taaggcgtcg agatcagcca tgctgccgca catatgcacg ggcatgatgg
     6901 cttttgtttt gggcgtaata gctttgcgta cggctgccgg atcgagggtg agggtatcat
     6961 ccacatccac caataccggt acggcaccca cactcagtac ggcttcaaaa gaagcgacaa
     7021 acgtaaaagc cggcgtgatt acttcatcgc catagccaat accgagcgca ctcatagccg
     7081 tggttaatgc ggaagtgccg ctggaggtca gctgagcata tttgctgcca aaggtttcgc
     7141 agatggccgc ttccagttct ttggatttcc agatcccctt acgcggaccg tcaaatccat
     7201 accgcatcag gatcccggtt tccaggacat catttacttc tttccgttct ttatcactcc
     7261 agagttcgta gccaggcata gtagtttagt tagtagttag aagttcgtag ttagtagtcg
     7321 ggaagactcc gggaagggag agtgcctgac tggggggcaa aggtaaaaaa gaaagtcggt
     7381 agtcggcagt caggagtcgg tagcctgcct gccggcaaag gcaggtcgga aaggcggctc
     7441 aggcaacgga aaggcgaatt ggcatgtcag tcaataaaat tgccatgaga tccatttttg
     7501 taattttttt catgctttac ttcctgccag ctgaagcaca gtggaggctg gggtttaggg
     7561 ccggattgaa cggatccaac ctggtgcatc gttcggaccg gcatgataaa cggaccacgg
     7621 gtgcggttgt ttcaagattg aatggcggac tgctgttaga aatacccctg gaagatcaat
     7681 ggtatattaa tacaggtatt cattattccg gaaaaggggc aaatatgttt gcgtctgatt
     7741 ccaccatgaa aaaagattct gaacgggtca ggttgaatta tctagaaatc cctgtaatgg
     7801 tggtgtataa atttcccagc gaaaaggaaa accagtttat tctttcatcc ggtccatata
     7861 ttggttatgg gtttaatggt acaataaact ggaaaggggg atttattcca acaaaagatc
     7921 aggttcatcg taaagaagaa gaggaatacc gccgttttga actgggctgg atgatttccg
     7981 ctatctacga gattaaatca agatatggcc tgcggctaga tttttccaaa agcctgctga
     8041 atatccagca tccggaaacc aggcagaaga aca
//
LOCUS       DAOPMW010000123         8047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_9982_length_8047_cov_7.297422, whole genome shotgun sequence.
ACCESSION   DAOPMW010000123 DAOPMW010000000
VERSION     DAOPMW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_8047_cov_7.297422"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLU11_07395"
     CDS             complement(<1..697)
                     /locus_tag="PLU11_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPN58979.1"
                     /translation="MEYRIEKDTMGEVKVPADAYYGAQTQRSIDNFKIAQDINRMPKE
                     IIEAFAYLKKAAALTNYDAGVLAKNKSVLIGKVCDEILKGKLNDYFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLKGGQLTDKEKFLHPNDDVNKSQSSNDTFPTAMHIAAYKILVE
                     TTIPGITKLRNTLAAKSKKFMKVVKIGRTHFMDATPLTVGQEFSGYVSQLDHGLKAIK
                     NSLAHLSELALGGT"
     gene            831..2645
                     /locus_tag="PLU11_07400"
     CDS             831..2645
                     /locus_tag="PLU11_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPN58980.1"
                     /translation="MKRIILFSVFLFIAMPVFSQGKHQFALGKQDFLLDGKPLQLISG
                     EMHPARIPKEYWRHRIQMAKAMGCNSIAAYLFWNYHEVKPGVFDFKTENRDIAEFVRI
                     CQQEGMWVLFRPGPYVCAEWDFGGLPVSLLKIPDIKIRCMDPRYMKAVESYISAMARQ
                     VKSLQVTAGGPILMVQIENEYGSYGNDKAYLERLRQLWLSNGINVPFYTADGPTPYML
                     EAGNIDGAAIGLDSGSSEADFEQAKKRNPNVPAFSSETYPGWLTHWGEKWQRPDTNEL
                     KKEVEFLLKTGRSVNFYVIHGGTNFGFTAGANAFSPTAYQPDITSYDYDAPINEQGQP
                     TAKYFLLRNLISKYSAAPLPDIPAPQRVIYVQEIPMKRVQSIWDVLPPPIISPQPLPM
                     EMLDQNQGLMLYRTELIGHKSGKLTIWEPHDYALVFLDGVFIDTVFRDGGKWTVDLPK
                     TTTTQNPVLDILVEGMGHINFAQFMIDRKGITDRVTLNGMTLMNWQHYSLPMSEAFVA
                     KALKQPRFDARGRNGQFFTGQFTLEETGDTFFDLSDYGKGMVYVNGHLLGRFWNIGPQ
                     KRLYCPAAWLNKGRNEMVIFDLHRNTASAVVGVQSMNE"
     gene            2859..5117
                     /locus_tag="PLU11_07405"
     CDS             2859..5117
                     /locus_tag="PLU11_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58981.1"
                     /translation="MPILKPLPLLFSFVLLANCIAAQPQPAHDLQFNQLPQRWDEGIP
                     LGNGWLGALIWQKENKLRLSLDRVDLWDDRPMPEIDKLKFSWVVEKVIRNQYDSVQKI
                     GDEPYEKYPAPTKIPGAAIEFDLAKLGKLSSGRLHINNGLSTMLFENGVVFNNYIHAT
                     RETGYFGFENCTIDMIPELMIPAYNSGKAGGTGSSVEGQGLERLGYAKGTVTKTHSSI
                     RYHQPTYQGHYYEVLVQWFRPAKYRVIGQWTISNDKPAVLIKPDFTKAEPTFWPTHIA
                     WWKDYWSRSSLRIPDEKIERQYYLDMYKFACVARGNTPPISLQAIWTADNGNLPPWKG
                     DFHHDLNTQLSYWPGYASNHLDLTAGYTNWLLKVRKENERWTRQYFELPGLNVPGVTT
                     ISGKEMGGWIQYSMSPTTSAWLAQHFYWQAMFSKKRTDYEQALAWLTDVELFLYRYLE
                     KEELDPSFIALSSSPEYHDNSVKAWFRYTTNYDKALVKFVRYAKQQLIQHFNQFFPPV
                     ILAEEDAIEKLGAFDANETGLTVAPNQNLDQSHRHLSPYMAIYPLCLLNVDKAADKKL
                     IDLSLRHIEKLGTREWCGYSFSWMACMYARCREADSAVSMLNKFASNFVSSNSFHLNG
                     DQKGGQYSNFTYRPFTLEGNFAFAQGVQELLIQTREGVIEVFPAIPERWKDVSFRTLR
                     AERAFLVSAVKENGVVKEVVVKSEAGGTCLLRLPFKNWTITGIDRSKVKVRDGVMMVP
                     MSKGQSVRMSNE"
     gene            5249..6673
                     /locus_tag="PLU11_07410"
     CDS             5249..6673
                     /locus_tag="PLU11_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN58982.1"
                     /translation="MRIIKPLSLFLSFILLANCILAQPKPSASQLAWHDMEYYFFFHF
                     GPNTFTDLEWGHGTEPADMFNPTELDCRQWVRTAKAAGAKGVIITAKHHDGFCLWPSK
                     YSKHTVRESKWKNGKGDVLKELSAACKEYGLKFGVYISPWDRNHPDYGTEKYNDVFVN
                     MMKELFTNYGPIWELWWDGANGEGPNGKMQVYDWARYKKTVKELSPNTVVFSDIGPHV
                     RWVGNESGIAGKTNWNTLDTVGFTPGKGGPTQDTLNTGNKFGKAWIPAECDVSIRPGW
                     FYHEAEDNKVRTPENLFDLWLKSVGRGANLLLNIPPDRRGKIHQNDSLALAGFRNLRD
                     RAFKSDLLSGRKLYFKIDNRGNGRADGSSVFIPKDKKSEFNKVPVHKNEQMISFSKPE
                     KINCIVLNERIANGQTITGFRIVIINGDVRSEITGTTIGHKRILSFPVTTAKSIEIYL
                     TDMQNRDNLQTVNAYLINEQLLEK"
     gene            6679..>8047
                     /locus_tag="PLU11_07415"
     CDS             6679..>8047
                     /locus_tag="PLU11_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN58983.1"
                     /translation="MKKNLLAICLLINTIAQAQPYTPDWESLDKRPVPNWFSNDKFGI
                     FIHWGMYAVPGYTAKGNYSEWYQQYMQGRKDSAVLKYHQAKFGDRNYYDLARDFKAEL
                     FNPDEWAKLFEKSGAKYIVLTSKHHDGYSLWPSEETRKAWSFPWTSADVGPKRDLIGD
                     LFTAVRKTTVRAGMYYSLYEWFNPLWKADPAKFAEQHTWPQMKDLINRYQPDVFWTDG
                     DWDAPAELWKSQQFLAWLYNESVVKEKVVVNDRWGAGIRFKHGGIFTPEYQPDLDFED
                     HAWEESRGMGFSYGYNREEDAWDYNSTQSLILQLIDKVSRGGNFLLDIGPDEHGKIPP
                     IMQDRLLQMGEWLKLNGEAIYNTRRWKTTSQWSAGRRDYTDRHGDMLLKITVDPDPGY
                     AVKEVFYTYNPTANNLYAILPRYPQNRKMVLKDIQLPVGTKLDLLSTGQSLVWRQTGA
                     NVEVQL"
BASE COUNT         2195 a         1755 c         2020 g         2077 t
ORIGIN      
        1 ccgtaccacc cagggccagt tcgctcaggt gggccagtga gtttttaatg gctttcagtc
       61 catggtctaa ctgggataca taaccgctga attcctgtcc tacggtgagg ggagtagcat
      121 ccataaagtg ggtccggcca atctttacca ctttcatgaa cttcttgctt tttgcagcca
      181 gcgtgttgcg gagtttggtg ataccaggga tcgtggtttc caccaggatc ttgtaggctg
      241 caatatgcat ggccgtgggg aaagtatcgt tggaggactg cgatttattt acatcatcat
      301 tcgggtgcag gaatttttct ttatcggtca gttgaccgcc tttcagtaca tgtccccggt
      361 aagcgatcac ttcatttaca ttcatattgc tctgggtacc actgccggtc tgccatacca
      421 ccaacgggaa ataatcattc agtttacctt tcaggatctc atcacagact ttaccgatca
      481 gtacggattt attcttcgct aatactccag cgtcataatt cgtcagggcc gccgcttttt
      541 tcaggtaagc gaatgcctca atgatctctt ttggcatgcg gttgatatcc tgggcaattt
      601 tgaagttgtc aatactgcgc tgagtctgtg caccatagta ggcgtcggcc ggaaccttca
      661 cttcacccat ggtgtctttt tcgatcctgt attccataat taagggattt ttaaaatttc
      721 cgcaaaggta gttcaaagag gcaaacggtt gcttcaaatt tatcgggtac tgtactgcac
      781 cggggagaat gccttatttt agtatatcgc aacccaccta aattgctatt atgaaacgaa
      841 tcatactatt ttctgtcttt ttattcatcg ccatgcccgt cttctcacag ggcaaacatc
      901 agtttgcact gggtaaacaa gacttcctgc tcgatggcaa acccctgcag ctgatcagtg
      961 gggagatgca cccggcccgg ataccaaaag agtattggcg gcaccggatt cagatggcca
     1021 aagccatggg ttgtaattcc attgcggctt atttgttctg gaactatcat gaggtaaaac
     1081 ctggtgtatt tgattttaaa acagagaacc gcgatatcgc ggagtttgtc cgtatctgtc
     1141 agcaggaagg aatgtgggta ttgttccgtc cgggacctta tgtctgtgcc gaatgggatt
     1201 ttggcggctt accggtatcc ctgctgaaaa taccggatat aaagatccgt tgcatggatc
     1261 cccgctatat gaaagccgtg gaaagctata tcagcgccat ggcacggcag gttaaatctc
     1321 tccaggtcac cgctggcggc ccgatcctga tggttcagat cgaaaacgaa tacggcagtt
     1381 acgggaatga taaagcctac ctggagcgct tacgtcagtt gtggctgagt aacggaatca
     1441 atgtaccctt ttatacggcc gatggtccta ctccctatat gctcgaagcc ggtaatatcg
     1501 atggcgccgc tattggactt gacagcggca gcagtgaagc tgattttgaa caggcgaaaa
     1561 aaagaaaccc caatgtaccg gctttcagca gtgagaccta tcccggatgg cttactcatt
     1621 ggggcgaaaa atggcaacgg ccggatacga atgaactaaa aaaggaagtt gagtttctat
     1681 tgaagaccgg gcgttctgta aatttttatg tgatccatgg ggggaccaat tttgggttca
     1741 ccgccggtgc caatgccttt tcgcccacgg cctaccaacc ggatatcacc agttatgatt
     1801 acgatgcccc gatcaatgaa cagggacaac ccacggctaa atattttttg ctccgcaacc
     1861 tgatcagtaa atacagtgca gcacccttgc cggatatccc tgctccgcaa cgggtgatct
     1921 atgtgcagga aattccaatg aaacgggtcc agtctatctg ggatgtgctg ccaccaccga
     1981 ttatcagtcc gcagcctctg ccgatggaaa tgctggatca gaaccaggga ctgatgctct
     2041 accggaccga actgatcggt cataaaagcg gcaaactgac catctgggaa ccgcatgatt
     2101 atgcgctggt gtttttagat ggggtgttta ttgatactgt attccgggat ggcggcaagt
     2161 ggacggtgga tctacccaaa acaactacta cccaaaaccc ggtattggat atactggtgg
     2221 aaggaatggg acatatcaac tttgcccagt tcatgatcga ccgtaagggt atcaccgacc
     2281 gggtgaccct gaacggcatg accctgatga actggcagca ttattcctta cctatgagcg
     2341 aggcttttgt ggcaaaggca ctcaaacaac cccggtttga tgccaggggc cgcaacggcc
     2401 agtttttcac cggccagttt acgctcgaag aaaccgggga tacttttttt gatctcagcg
     2461 attatgggaa agggatggtc tatgtaaacg gacacctgct cgggcgtttc tggaatatcg
     2521 gaccgcaaaa gcgattatac tgtccggccg cctggttgaa caagggcagg aatgaaatgg
     2581 tcatttttga tttgcaccgc aatactgctt cggcggtggt gggggtgcag tcgatgaatg
     2641 agtgaggggg cttgcagata gaagcagtct gaaagaagag atgagtaatg agtaatcagt
     2701 aatgagtaat cagtaatgag taaaagggaa gatactttac agctttgata caggtactta
     2761 tttgtaaaga cttttccgga gctacctgcc tttgtcggca ggcaggctca ttactcatta
     2821 ttcattactc attaaaaaaa acaaactaat tctcccatat gcccatcctc aaaccccttc
     2881 ccctcctttt ctcttttgtc ctcttagcca attgtattgc tgctcaaccc caacctgcgc
     2941 atgatcttca gttcaaccag cttccgcaac gctgggatga aggaatacca ttgggcaatg
     3001 gctggctcgg tgcgttgatc tggcagaaag aaaataaact gcgcttgtcg ctggacaggg
     3061 tggatctctg ggatgatcgt ccgatgccgg agatcgacaa actgaagttc agctgggtgg
     3121 tggagaaagt gatcaggaac cagtacgact ctgtacaaaa gattggggat gagccttatg
     3181 aaaaataccc ggcacctacc aagatacccg gagcggcgat tgaatttgac ctggcaaagc
     3241 tgggcaaact gagttccggc cgtttgcata tcaataatgg tttaagtacg atgttgtttg
     3301 agaatggggt tgtgtttaat aattatatcc atgccacgcg ggagaccggt tattttggtt
     3361 ttgaaaattg tacgatcgat atgattcctg aattaatgat ccctgcatat aactccggaa
     3421 aggcaggagg caccgggagc agtgtggaag gtcagggact ggaacgactg ggttatgcaa
     3481 aaggaacagt tacaaaaaca catagcagta tccgttatca ccaacccacc taccagggac
     3541 actattatga agtgctggtg caatggtttc gtccggcaaa gtaccgggtc attggccaat
     3601 ggaccatcag taatgataaa ccagcggtgt taattaaacc tgactttacc aaagccgaac
     3661 ccacgttctg gcctacgcat atcgcctggt ggaaggatta ctggagccgg tcatccttac
     3721 gtataccgga tgaaaagatc gagcgtcagt actacctcga tatgtataag ttcgcctgtg
     3781 tggccagggg taatacacca cccatctcct tacaggctat ctggaccgcc gacaatggca
     3841 acctgccacc ctggaaaggc gactttcatc atgatcttaa tacacaactc agctactggc
     3901 cgggttatgc ttctaatcat ctggatctta cagccgggta taccaactgg ttattgaaag
     3961 tgcggaaaga aaatgaacgg tggaccagac aatattttga attacccggt ttgaatgtgc
     4021 ccggtgtgac caccatcagt ggtaaggaaa tggggggatg gatccagtat tcaatgagtc
     4081 cgaccacatc ggcctggctg gcgcaacatt tttactggca ggcgatgttt agtaaaaaaa
     4141 gaacagatta tgaacaggca cttgcatggt taactgatgt ggaacttttt ctttaccggt
     4201 acctggagaa agaagaactg gatcccagct ttatagccct gagttcaagt ccggagtatc
     4261 acgataattc ggtgaaagcc tggttcaggt ataccacaaa ttatgataag gccctggtca
     4321 agtttgtaag gtatgcaaag caacagctca ttcagcattt taatcagttc tttccgccag
     4381 tgattcttgc tgaggaggat gctattgaaa aactgggtgc tttcgatgcc aatgaaaccg
     4441 gtctgaccgt agcacccaat caaaacctcg accaatccca tcgtcatctc tctccttata
     4501 tggcgatcta cccgctttgt ttactgaatg tcgataaagc agcagacaaa aaactgatcg
     4561 atctttccct tcgtcatatc gaaaaactcg gtacccgcga atggtgtggt tactcgttca
     4621 gctggatggc ctgtatgtat gcccgttgcc gcgaagccga tagtgccgtg agtatgctga
     4681 ataaattcgc ctccaatttt gtttcttcca atagctttca cctcaacggg gatcagaaag
     4741 gcggtcagta ttcaaacttt acctaccggc cttttacgtt ggaaggtaat tttgcctttg
     4801 cccagggtgt acaggagtta ttaatacaaa caagggaagg agttatagaa gtatttcctg
     4861 ctataccgga aagatggaag gatgtatctt tccgtacgct tcgtgccgaa agggccttcc
     4921 tggtatccgc tgtaaaagaa aatggggtgg tgaaggaagt ggtggtgaaa agtgaggcgg
     4981 gaggcacctg tttgctgcgt ttgccattta agaactggac gattaccggt attgacagga
     5041 gtaaggtgaa ggtgagggat ggggtgatga tggtgccgat gagtaagggg caatccgtga
     5101 gaatgagtaa tgaataatga gtaatgagta aatgggaaga tactttacag ctttgataca
     5161 gttactaatt cgtaatgact tttcccgggc tactcattac tcatcctgaa aagtttcaca
     5221 ttttatcaga ataaaccaat tcaaccatat gcgtataatt aaacctcttt ccctctttct
     5281 ctcttttatc ctcttagcta attgtattct ggctcagccc aaacccagcg cctcccagct
     5341 cgcctggcac gatatggaat actatttctt cttccatttc gggcccaata catttaccga
     5401 tctggagtgg ggacatggga cggagccggc ggatatgttt aatccaacgg aactggattg
     5461 ccgccaatgg gtacgtacgg cgaaagccgc cggagccaag ggcgtgatca ttaccgccaa
     5521 acaccatgat ggtttttgtt tatggcccag taaatattcc aaacatacgg tgcgggagag
     5581 caaatggaaa aacgggaagg gagatgtact gaaagaactt tcggcagcct gcaaggaata
     5641 tggattgaag tttggtgttt atatttctcc ctgggaccgc aatcatcctg attatggtac
     5701 tgagaaatac aatgatgtat ttgtgaatat gatgaaagaa ctcttcacca actatggtcc
     5761 catctgggag ctctggtggg atggtgccaa tggagaaggt cccaatggaa aaatgcaggt
     5821 gtatgactgg gcccgttata aaaagacggt aaaagaactt tcaccgaata cggttgtctt
     5881 tagtgatatc ggaccacatg tgcgttgggt gggaaatgaa agtggaattg ccggcaagac
     5941 caactggaat acactggaca ccgttggatt caccccgggc aaaggcggcc ccacacagga
     6001 cacattaaat actggaaaca aattcggcaa ggcctggata ccggctgaat gtgatgtgag
     6061 catccgtccg ggctggttct atcacgaagc ggaagacaat aaagtaagaa cgccggaaaa
     6121 tttatttgat ctctggttaa agagtgtggg cagaggagcg aatctgttat tgaatattcc
     6181 accggaccgg agagggaaga ttcatcagaa tgattcgttg gcgttagctg gattcagaaa
     6241 tctgcgtgac cgggctttta aaagtgattt attaagcggg cgaaaattgt attttaaaat
     6301 cgacaaccgg ggtaatggaa gggctgatgg aagcagtgtt tttattccta aagacaagaa
     6361 aagtgaattt aataaagttc cggtacataa gaatgagcaa atgatttcgt tttcaaaacc
     6421 agaaaaaatt aattgtatag ttctgaatga aaggatagca aacggtcaga cgattacagg
     6481 ttttagaatt gtgattataa atggagatgt tcgatcagaa attacaggaa ctacaatcgg
     6541 gcataaaaga attctttctt ttcctgtaac tactgccaaa agcattgaga tatacttaac
     6601 ggatatgcag aacagggata atttgcagac tgtaaatgca tatttaatta acgaacaact
     6661 gcttgaaaaa taattaaaat gaaaaagaac ctgcttgcca tttgcctttt gattaacacg
     6721 attgcacagg cacaacccta cacccccgac tgggaaagtc tcgacaaacg tcccgtgccc
     6781 aactggttca gcaacgataa gtttggcatc ttcattcact ggggtatgta tgccgtgccg
     6841 ggttataccg ccaagggcaa ttattcggaa tggtatcagc agtatatgca ggggcggaaa
     6901 gacagtgctg tactgaaata ccaccaggcg aagttcggcg accggaatta ttatgacctg
     6961 gcaagggatt tcaaagccga actctttaat cccgatgaat gggctaagct ctttgagaag
     7021 agcggggcaa aatatattgt actaacgtcc aaacaccatg atggttattc cctatggccc
     7081 agtgaggaga cccggaaggc atggagtttt ccctggactt ccgcagacgt aggacccaaa
     7141 cgtgatctga ttggtgatct gtttaccgcg gtgcgcaaaa caaccgtacg ggctggtatg
     7201 tattattccc tctacgaatg gttcaacccg ttatggaaag cggatcctgc caaatttgcg
     7261 gagcagcata cctggccaca gatgaaagat ttaatcaacc ggtatcagcc cgatgtattc
     7321 tggacggatg gagattggga tgcgccggct gaattatgga agagtcagca gttcctcgcc
     7381 tggttgtaca acgaaagcgt ggtgaaagag aaagtggtgg tgaatgatcg ctggggtgcc
     7441 gggatccgtt tcaaacacgg gggcatcttt acccccgagt accagcccga tctcgatttt
     7501 gaagatcatg cctgggaaga aagccggggc atgggtttca gttatggcta taaccgcgaa
     7561 gaagatgcct gggactataa cagtacgcaa tcactcattt tgcagttgat cgataaagtc
     7621 agccgcggtg gaaatttctt actcgatatc ggtcccgatg aacacggcaa gatcccaccc
     7681 atcatgcagg accgtttatt gcagatggga gagtggttga agctcaacgg cgaagcgatt
     7741 tataataccc gtcgctggaa aacgaccagt cagtggagtg ccggccggag agattacacc
     7801 gatcgtcatg gtgatatgct gctcaagatt acggtggatc ctgatccggg ttatgcagta
     7861 aaggaagtat tctataccta taatccaacg gctaataatc tctatgcgat tctaccccgt
     7921 tatccgcaaa accggaaaat ggttttaaag gatattcaat tacctgtggg tacaaaactg
     7981 gatctgttat ccaccggtca gtccctggta tggagacaaa ccggtgccaa tgtggaagtg
     8041 caattgc
//
LOCUS       DAOPMW010000124         8030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10020_length_8030_cov_8.047524, whole genome shotgun sequence.
ACCESSION   DAOPMW010000124 DAOPMW010000000
VERSION     DAOPMW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10020_length_8030_cov_8.047524"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PLU11_07420"
     CDS             <1..1112
                     /locus_tag="PLU11_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPN58984.1"
                     /translation="KSNADEFNKYKSKLPADVAVALYLLNDKTLMIFTLTNEKLAVDT
                     MTVDLAPRISRFIEAIKNTGKQTGTGPLSLRSDPVDEDKTASTAAFTDLSGDLYQVLI
                     QSVADKIKGKEKLCIIPTGVFSNLPFQCLGRKQAGTAFRFLIEDHTIFYTNKMSVFSD
                     SMQLPVAQNNYSFAAFGVPDATLRFNISEVKTIGKMLGSDSTVYTDTRATESMAKQSL
                     VTKKYIHFATHGVLNYSSDYSMSYLKLLPDKDSTQGNNGKLTMREIQRLGITDCSMVI
                     LSACQTAVSKELVKGWSISPANSFLISNVKTVVASLWKVADEPTSLLMQYFYENLPGM
                     GKAEALRQAQVRLSKDPRFVHPNYWGAFVLYGDWR"
     gene            1182..2558
                     /locus_tag="PLU11_07425"
     CDS             1182..2558
                     /locus_tag="PLU11_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HPN58985.1"
                     /translation="MKKYVVLILFAAIAGTQVNAQIKALGDKKPAKSPCTTLVGDEML
                     PGMADTTTGRSVSDNYKTWENGAVLLVKFMNNTGSPAIRERITRYAKEWEKFANIKLN
                     FVPDNTPTTNIRIRLGSRRDKLGHNSAVGIDNDQRAQARQTMNLDTSDFLDHDFFLND
                     LKTGGPFYKFLVAKGKDMKSYTYPQFYNDVIDFPSPLPRFAEKVAKGTTMHEFGHALG
                     LLHEQSYPGAIKWNKDTVYKYYEQYDWDKAKTDFNVLEISEQFFTNGTAYDPKSIMHY
                     SIPAWQTVDGYSVDENYEMSDGDKKIIAAIYPKDKKISALAVPKVTVSNFVKLEVKKD
                     DVKKALIITPYFNVRTGAKLANAYYVARLTTEDGQYYIATKSLKFNWGGFSGTYLKMN
                     LLPNTKKTYNKLAKKDLQLVLPYKEIPELAGKNFRVEFTVYQNVVATGKLDRFVTYSL
                     SSPLSLVR"
     gene            2750..2823
                     /locus_tag="PLU11_07430"
     tRNA            2750..2823
                     /locus_tag="PLU11_07430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2784..2786,aa:Pro,seq:tgg)
     gene            3643..6189
                     /locus_tag="PLU11_07435"
     CDS             3643..6189
                     /locus_tag="PLU11_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN58986.1"
                     /translation="MINSPEPGKIPQFPTPYVQYTCQIRNSTFSILSVSEKIFSLTGF
                     TPEEWCSSNNVLFNIMHPGDRERVFNAYNSISARNNELSIQYRLIDKNGITRWIQDIA
                     ILTKKDENGTLELKGYLYDVSGKKKITEIVKTLKAYQFALNESAIVSLTDLEGRIIYV
                     NENFIYYSGYTRHELIGRTHRIINSGYHPADFFSNMWKTVRTGKSWRGEVRNRAKDGT
                     YYWVDTVITPVFNEKKEITQFLSIRNLISKQKEQEEAIKQSEEKFRDIVESTSDMIQS
                     VDGNGLIVFVNKSWSNRLGYTAENVVGKPVINFITPERKDHWSQIFHTIRSGTEVRSA
                     EVIFITKDGERVFCEGNINSRYENGTMVLTRGIFRDISSARKLMETLERNQGQLKEAQ
                     RLAQVGSWHYDTGLNLLEWSEEACRIFEVSDESQISYELFLSSVHPDDRDLVNEAHAE
                     ALKGEPYNLEHRLFINGKIKWVKSIIQIRFDNNHEPLIYSGSVQEITEKKLAEIEIFK
                     HQHQLKEAQRTANIGNYEWDTVNNKVSGSDHFYQLLGLRKKKCYDFDLLFNNVHPDDR
                     ESLQKVIQKSIQSSESFSIQFRYITPDGMIKHFEAIRQSHNYQSCEKNYFAGTIQDIT
                     ELKKTEQKLFNSIIEAEENERKRIAAELHDGVCQYLATSKLLLQALDTNSDQPLSSQK
                     PILDRIFATVNESLRLTRNVSQQLVPLILYEEGLLSAIQELISTLNLVDSQRYHICTE
                     GRPVDPEPHFSINLYRIVQEFINNSQKYSEASSIKVKILYSVNALEIHLSDNGCGFDL
                     EQVKKQKGIGLFNMTNRICSIGGRFRFASRPGKGVSLKLYIPVSKYSSKG"
     gene            6583..7872
                     /locus_tag="PLU11_07440"
     CDS             6583..7872
                     /locus_tag="PLU11_07440"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN58987.1"
                     /translation="MSATLLFFKVKSTSRLSHIAVFSVLLLLGEIKVSQAQVNSYTFS
                     TSTGNVLETGGFTNLLNNFLDDDVSAMANIGFTFRFAGTNYTNFSVTSNGLMQLGGSA
                     VTDYNNVTGNLTGPYLIPYWDDNYTDADGNVQYKVMGVAGSRKLVVEFNLSYLGNTGA
                     ADKRFQVWLFETTNRVHFVYGAGNNFNGGYSIGILTNGLTDFISVSVASNTRSIVTAN
                     DNNTTWPGSGRAYSFINSNTLPVTLLNFSGYRSGSRNQLQWTTVTEINNQGFELQRSA
                     DGNNFTTIDFIQSQSTGGNSNVQLSYLYTDNSISTGKQYYRLRQLDIDGQGRFSNVIK
                     IDGQIPSEVRVDGVFPNPVVTNTLNLQLTSPNRQSLAIYLYDMSGRIVLSRNIPVETG
                     SNTIPVDISQAATNLYQLKIMDSRGYNCGNIKVSVVK"
BASE COUNT         2451 a         1579 c         1796 g         2204 t
ORIGIN      
        1 gtaaatcaaa tgcggatgaa ttcaataagt ataaatcaaa actgcctgcg gatgtagccg
       61 tggccctgta cctgctgaac gataagacgc tgatgatttt taccctgact aatgaaaagc
      121 tcgccgtgga taccatgacg gtggacctgg ctccccgcat cagccgcttt atcgaagcga
      181 tcaagaatac cggcaagcag acgggtaccg gtcctttgtc cctgcgctct gatccggtgg
      241 atgaggacaa aacagccagc acggcggctt ttaccgatct ctccggtgat ttgtatcagg
      301 tgctgattca atcggtggcc gataaaatca aaggaaaaga aaagctttgc atcattccta
      361 ccggggtatt cagtaacctg ccatttcagt gtcttggtag aaaacaagcc ggaacagctt
      421 ttcgtttcct catcgaggat cataccattt tttatactaa taaaatgtca gtcttctccg
      481 acagcatgca actgcccgtg gcgcagaata attattcttt tgcggcattt ggggtgcccg
      541 atgccacgct taggtttaat atcagtgagg taaagacgat cgggaaaatg ctgggttcgg
      601 attccaccgt gtatacggat acaagagcta cggaaagcat ggctaaacaa agcctggtta
      661 ccaagaagta tattcacttt gccacgcatg gagtactgaa ttattcctct gattattcga
      721 tgtcgtacct gaaattgttg ccggataaag acagcaccca gggcaataac gggaaattaa
      781 ccatgcggga gattcagcgg ctgggcatta ccgattgcag catggttatt ttatctgcat
      841 gtcagacagc agtgagcaag gaattggtaa agggctggag tatttcaccg gctaattcat
      901 ttctcatcag taatgtgaaa acggtggtgg ccagcctgtg gaaagtggcc gatgaaccca
      961 cgagtctgct gatgcaatat ttttatgaaa acctgcccgg catggggaaa gcagaggctt
     1021 taagacaggc gcaggtaagg ctcagtaaag acccccggtt tgtccatccc aattactggg
     1081 gcgcctttgt gctatatggg gactggcggt aaaataccct tggtagggta tgccgtatga
     1141 attaagtaaa tattacattt atcttataaa ctgaaattga aatgaaaaaa tacgttgttt
     1201 taatcttatt cgctgcgata gccggcaccc aggttaatgc gcagataaaa gccttaggtg
     1261 ataaaaagcc agccaaatcc ccctgtacca cactggtggg ggatgaaatg ttgccgggta
     1321 tggccgatac gactaccgga agatcggtgt cggacaatta taaaacctgg gaaaacggcg
     1381 ctgtactcct tgttaaattc atgaacaaca cgggtagccc ggccatccgg gagcgcatta
     1441 cccggtatgc aaaggagtgg gagaaatttg ccaatatcaa attgaatttt gtaccggata
     1501 atacccccac caccaatatc cgtatccgtc tgggcagccg gcgtgataaa ctcggacata
     1561 attccgccgt gggtattgac aatgatcagc gtgcgcaagc ccgtcaaacc atgaacctcg
     1621 ataccagtga tttcctggat catgattttt tcctgaatga tttaaaaacc ggcgggcctt
     1681 tttataagtt cctggtggca aaaggaaaag acatgaaaag ctatacctat ccccagttct
     1741 ataatgatgt aatcgatttc ccatctccgt tgccccggtt tgctgagaaa gtggccaaag
     1801 gcaccacgat gcatgaattc ggtcatgcac tgggtttatt acatgaacaa agctatcccg
     1861 gtgcgataaa atggaacaag gatacggtat acaagtatta tgagcagtat gactgggata
     1921 aggcgaaaac agatttcaat gtactggaga tcagtgaaca gtttttcact aatggtaccg
     1981 cgtatgaccc caaatcaatc atgcactatt ccattcctgc ctggcagacg gtggatgggt
     2041 attcggtaga tgaaaattat gaaatgtcag atggcgataa gaaaatcatc gctgccatct
     2101 atccaaaaga taaaaaaata tcggcactgg cagtgcccaa agtgactgtt tcaaattttg
     2161 taaaactgga agttaaaaag gacgacgtaa agaaggccct gatcatcaca ccctatttta
     2221 atgtcagaac cggtgcaaaa ctggcaaatg cctattatgt ggcccggtta accaccgagg
     2281 atggacaata ttatattgcc accaaaagcc tgaagtttaa ctggggtgga ttcagtggta
     2341 cctatcttaa aatgaacctg ctgcccaata cgaagaagac gtataataaa ctggctaaaa
     2401 aagacctcca gctggtattg ccttacaaag aaatacccga actggccggc aaaaatttca
     2461 gggtagagtt tacggtgtac cagaatgttg tggcaaccgg caaactggat cgttttgtta
     2521 cgtattccct gtcttcgcca ttgagcctgg taagataagt tcagacaccc gggattaatt
     2581 aattacagct gtttcatcag tttttcagta tgcgccaatg caagggctgg tttaacctac
     2641 tgaaagacag ttgaaacagc cttttttttt ctttttgatt ccataaagaa atctccggct
     2701 tttaacccgg aagcctgtat atttgcactc ccctgaaata cggggtgtgc gggaggtagt
     2761 tcagcccggt agaatacctg gtttgggacc agggggtcgc aggttcgaat cctgtcctcc
     2821 cgacagtata actagttctc tagtttaaaa aatgccgtaa atcattgatt tacggttttt
     2881 ttatgcatat aggtctgtat ttactttcca gtgactctgc cggcaggtga attagccggt
     2941 gagcgacttt caactatcct ttctgtctgc atataagtcc gctattacac aaaacatagg
     3001 tattaatgag caggattgtt acatcataaa atgtgtacat agaggaagaa ctttaattaa
     3061 tttcctgtgg ttaattctcc gttatgcttc tgttgacatc cagtaagttt cgaactgagt
     3121 aagagaatct ttcaataaat gatggattga gaattatctg aaccgttcaa ttaattccta
     3181 aaccgggtta aaaaaaagac aaagcctgat caggcaaaaa gaacccggtt tctgccgctg
     3241 cgattatatg atgctgtatg cgtgagcaca aaccgctgaa taccttttga ttttttttcc
     3301 tgctgcccgg aaaacgatga agcagtattg ctgcttgttc ggacaaagaa aacatatgcg
     3361 ttatagttgg aaagctgtat gtagtttaaa aactacatgc agtattgtct gatgacaaat
     3421 aatagtaatc tatggttaga attaaccatt tttagccgag cgataaatgt gtaagtaata
     3481 cataaatcta tttacatgat aaaaagaatt gatttaaatc agtggattta catttaaaac
     3541 aaaataatga caataatgct tgaagaaaaa ttgcttttgt tgaactgaat ttgctaaaat
     3601 tacagttcaa atgaaacaac caccactatt ttaaaatccg aaatgattaa ctcacctgag
     3661 cctggaaaaa tacctcagtt tccaacacca tatgttcagt atacgtgtca gatcagaaat
     3721 tcaactttta gtatattatc tgtttctgaa aaaattttca gtctgacagg ctttactccg
     3781 gaggaatggt gctccagtaa taatgtcttg ttcaatatca tgcatcctgg tgacagggaa
     3841 cgggtcttca acgcatacaa ttccatttcc gcaaggaata atgaactgtc tatccaatac
     3901 aggctgattg acaaaaacgg tataacacgt tggatacagg atattgcgat cctgacaaaa
     3961 aaagatgaaa atggcaccct tgaattgaaa gggtatctgt atgatgtgtc gggaaaaaag
     4021 aaaattactg aaattgttaa gacactaaaa gcataccagt ttgctttaaa cgaaagtgca
     4081 attgtatcgt taaccgattt agaaggccgg attatttatg taaacgaaaa ttttatatat
     4141 tattcaggtt atacccggca cgaactgatt ggcaggacac accgtataat aaacagtggt
     4201 taccacccgg ctgatttctt ttcaaacatg tggaaaacgg tccggacggg gaaatcctgg
     4261 agaggtgaag tcaggaatag agccaaagat ggcacctact actgggtcga taccgttatt
     4321 acgccggtat tcaatgaaaa aaaggaaata actcaatttc tttccatccg caacctgatc
     4381 agcaagcaaa aggagcagga ggaggctata aaacaaagtg aggaaaaatt cagggatatt
     4441 gttgaaagta cttcggatat gatacaaagt gttgatggaa acggtttaat tgtttttgtc
     4501 aataaaagct ggagtaacag gttgggatat actgccgaaa atgtggttgg taaaccggtt
     4561 ataaacttta tcacccccga gagaaaggac cactggagcc agattttcca tactattagg
     4621 agcggaacgg aagtaagatc cgccgaagtg atttttatta ccaaagatgg cgaaagagta
     4681 ttttgtgagg ggaatattaa ttcccggtac gagaatggaa cgatggtgtt gaccagaggt
     4741 attttccggg atatcagttc agccagaaaa ttaatggaaa cacttgaaag gaaccaggga
     4801 caattaaaag aagcccagcg acttgcccaa gtgggatcct ggcattatga taccggtttg
     4861 aatctcctgg aatggtctga ggaagcatgc cggatctttg aagtcagtga tgaatcccaa
     4921 atatcctatg aactctttct gtcctccgta catccggatg accgggactt agtaaatgaa
     4981 gcacatgcgg aagccttaaa gggcgaacca tacaatcttg aacatcgttt attcattaat
     5041 ggtaaaataa aatgggtgaa atccattatc caaataaggt ttgacaataa tcatgaaccg
     5101 ttgatttata gtggaagtgt tcaggaaatt actgaaaaaa agttggcaga gattgaaata
     5161 tttaaacacc agcatcaact gaaagaggcg cagcgtactg caaatattgg taattatgag
     5221 tgggataccg tgaacaataa agtttccggg agcgatcatt tttatcagct gctcgggtta
     5281 cgaaaaaaga agtgttatga ttttgatctg ttatttaaca atgtacaccc ggatgatcgt
     5341 gaatcccttc agaaagtgat ccagaaatcc attcagtctt cggaatcctt ttccatccag
     5401 ttccgttata taactccaga cgggatgata aagcattttg aagctataag acaatcacat
     5461 aattaccagt cctgcgaaaa aaattatttt gccggtacaa tacaggatat tactgaatta
     5521 aaaaaaacag aacagaagtt atttaattcc ataatcgaag cagaagaaaa tgaacgcaag
     5581 aggattgctg ccgaattgca tgacggcgtc tgccagtatc tcgcaacttc aaaactgctt
     5641 ctacaggcac ttgatacgaa ttccgaccag cccctgtcct cccagaagcc tatactcgat
     5701 aggatatttg ctaccgtgaa tgaatcgttg agactgaccc ggaatgtatc ccagcaactg
     5761 gtccccctga ttctgtatga agaaggcctt ctttctgcca ttcaggaact aatttccacc
     5821 ttaaacctgg tggacagcca gcggtatcat atttgcactg aggggcgtcc tgtagatcct
     5881 gagcctcatt tttcaattaa cctatatcgt attgttcagg aatttattaa taacagccaa
     5941 aagtattccg aagccagcag tataaaagta aaaatactct attccgtcaa tgcgctggaa
     6001 attcatctgt ctgacaacgg gtgtggtttt gaccttgaac aggttaaaaa acaaaaaggg
     6061 attggtctgt tcaacatgac taaccggatc tgttcaatcg ggggacggtt caggtttgcc
     6121 agccggccgg gcaaaggggt ttctttaaaa ttatatattc cggtttctaa atacagtagc
     6181 aaaggttaac ggttaaatgt atggctacac caggggttgt tggccagtgc cggcaataag
     6241 taatttttgg aaatctgtag ccagcttact cagtaattga caggggctcc agttggattc
     6301 attgaaaaca agacttaaca gcagaaaggt ggtttaacct gcgtgaccgg ctcatgaagg
     6361 ttgtataatt ctggcttgat ataaatataa tatttttgat atgccattat ttatttcaag
     6421 atggttttaa gtattcttgc ttaatcagtt aagtggtttc cattaattac atttattagc
     6481 gttgctgaga tatatttttt ccgccggtta tgcgttttgt ttgcacaagt accaggccgt
     6541 ctgagcaagg gccatatttt taaaatccaa aatctgaagt gcatgtctgc aactctactt
     6601 ttttttaaag taaaatcaac ttcccgttta agccatatag ctgttttttc agttctctta
     6661 ttattgggtg aaataaaggt gtcacaggca caggtaaaca gctatacatt cagcaccagt
     6721 accggaaatg tgctggaaac aggtggtttc accaatttac tcaacaattt tctggatgat
     6781 gatgtatctg ccatggcaaa tattggtttc actttccggt ttgccggaac aaactatact
     6841 aatttcagcg ttacctcaaa tgggttgatg caattgggag ggtcggccgt cacagattat
     6901 aataatgtaa ccggtaatct gaccggcccc tatcttatac cctattggga tgataattat
     6961 accgatgcag atggcaacgt gcaatataag gtaatgggtg tcgcgggaag caggaaactg
     7021 gtggttgagt ttaatctgtc ttaccttgga aataccggtg cggccgataa acgtttccag
     7081 gtctggttgt ttgaaacgac caaccgggtg cattttgtat atggggccgg taataatttt
     7141 aatggcggat attccattgg aattttaacc aatggcctga cggattttat ttccgtaagc
     7201 gtagcttcaa atacaagaag tattgtaact gcaaatgata ataacacgac ctggccagga
     7261 tcgggcaggg cgtatagttt tataaacagc aatacactgc ctgtaacctt attaaatttc
     7321 agtggttaca ggtccggcag caggaatcag ctgcagtgga caactgtaac cgagatcaac
     7381 aaccagggtt ttgaactgca acgatccgcg gacggaaata actttactac aattgatttt
     7441 attcaatccc agtcaacagg cggcaacagc aacgttcagc tcagttacct gtatacggat
     7501 aacagcattt caacaggcaa gcagtattac cggcttcgcc agctggatat agatggccag
     7561 ggccgattta gtaatgtaat taaaattgac ggtcagattc cttctgaggt ccgggtggat
     7621 ggcgttttcc cgaatcccgt cgttaccaat accctcaacc tccagctgac ttcaccaaac
     7681 aggcagtcac tggccattta tttgtacgat atgtctggaa gaattgttct gtccagaaat
     7741 attcctgttg aaacgggtag taatacgata ccagttgata tcagtcaggc ggcaactaat
     7801 ttatatcagt taaagatcat ggatagccgg gggtataact gcggtaatat aaaggtgtcc
     7861 gttgttaaat aagcatggtc gtcagccata gaccgggcag gcaaagtact gaccatgatt
     7921 tccgggattt ccgaataaac tgcagcgtta agcagggaag gtatattgtt gtataaccgg
     7981 gcataaaagg ttgttttttg aaaaaacagc ccctgtactc acggggaagg
//
LOCUS       DAOPMW010000125         7973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10136_length_7973_cov_11.811695, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000125 DAOPMW010000000
VERSION     DAOPMW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_7973_cov_11.811695"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLU11_07445"
     CDS             complement(<1..516)
                     /locus_tag="PLU11_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN58988.1"
                     /translation="MKKQFIQLFTCLLIMTIQTGIYAQPKTVNPFGLVYGGAIKENVP
                     GKVNIHPVSYKLNGLTIAANVYTPAGFDTTKKYPVLVMAHPNGGVKEQVAGLYAQRLA
                     EQGYICIAADASYQGASEGQPRNTDKPANRIEDIRGMADYIARYKGADADRLGLLGIC
                     GGGGYALAAAQT"
     gene            complement(537..1637)
                     /locus_tag="PLU11_07450"
     CDS             complement(537..1637)
                     /locus_tag="PLU11_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN58989.1"
                     /translation="MAKNKNLNQSVRTSFAFFTAAVLLVACSGTKNSAGNKPLVIREQ
                     GSFAAGGTVIATPGKFDPYKPAAAGQTLHGDHAYVFYQIPVKARKYPMIMWHGFGQFS
                     KTWESTPDGREGFQQIFLRQRFPVYLIDQPRRGNAGRSTIAANINPTTDEQQWFGTFR
                     LGIWPAYYEGVQFARDTNTLNQYFRSMTPDVGPIDIRVNTDAAAALFTKTGPAILITH
                     SHSGGMGWLTAVKNQNVKAIASYEPGSGFLFPEGELPAPMGSAGGTLTASAIPLADFM
                     QLTKIPIIIYYGDNIPDKPSAHPGEDGWRVRLAMARLWRDVVNKHGGDVTVVHLPEAG
                     IKGNTHFPFSDLNNLEIARLLSKWLNEKKLDQ"
     gene            complement(1680..2756)
                     /locus_tag="PLU11_07455"
     CDS             complement(1680..2756)
                     /locus_tag="PLU11_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN58990.1"
                     /translation="MKRRNLFKAGLAVAGGGLLASVASAQKTGTPVTIVKGKRRLGQQ
                     LEVSSIGLGVQNMPRTYQTTIPSRPEMINIIRKAFENGVTLFDTAEVYGPLESERILG
                     EAVAPFRNKIVLETKFFRSRPEQLKEKVEGMLKRLRTDRIDLLYQHRVDREVPIEDIV
                     GAIKNLIDEGKVLHYGLSEPGLQTIRRAHATHPVTAIQNEYSLLWRGPEKAVLPLCEE
                     LGIGFVPWSPLGVGFLTGAIDANTRFAQGDIRGGEGRFTPENLPNNLALVALIKKWAE
                     QKSATPAQISLAWLLAQKPWIVPIPGTTQMAHMLENNGADQVRLSVDEWTSFNTDLDK
                     IEIKGLRLPQGVLNMSDVEAPEKK"
     gene            complement(2773..3258)
                     /locus_tag="PLU11_07460"
     CDS             complement(2773..3258)
                     /locus_tag="PLU11_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN58991.1"
                     /translation="MKSTIIGIFLLLISSQICFTQQTDSKAVEQEITRLSKQKWQWMA
                     DKNTDSLNLLFDEKSMFVHMGGSWGKTQELKVIKEGTIWYKKAEVYSVIVNTIGNTVI
                     LLNEIDLVAVVGGNEVVNPFMVTEVYIKDGNKWKMGSLTFSKLMRPVKLATPPAATPS
                     H"
     gene            complement(3468..>4859)
                     /locus_tag="PLU11_07465"
     CDS             complement(3468..>4859)
                     /locus_tag="PLU11_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPN58992.1"
                     /translation="IREADKFCADCAQAGFQRGADSISRSSSEDCLFLNIWAPAGTKK
                     GAKLPVMVWIHGGAFVFGSGAHPDFSGEQFAKKGVILITFNYRLGRLGFFAFPALSQE
                     YPEESKGNYGYMDQIAALKWVQKNIAAFGGNPANVTIFGESAGGVSVHSLLSIPAAKG
                     LFHKAIIQSGGGRDGVLTGRPIRKENADRYYPVSAETIGINFAKRYSIQGTDAEALTR
                     LRNLTVAQIVDGGQESNGQGGPVTYPGPVQDGQLVVETAQAAYMAGRQAGVSIIIGSN
                     SAEVPAGFVNARTKEDLFSLFGKMKNEAATVLDPGGDAEFARLLTMVNTDKVWAEPAR
                     FTARAFTDKKQKAYIYLFSYVSAPFQSRMRFGAAHASEIPYVFNTLRGVNGSPVLPND
                     LEVARMMNNYWINFAKTGDPNGQDLPLWPVYDPKNNGILEFQPDGKATGKPDPKKARL
                     DLIEKAVTSGNLQ"
     assembly_gap    4860..4959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5165..6076
                     /locus_tag="PLU11_07470"
     CDS             5165..6076
                     /locus_tag="PLU11_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN58993.1"
                     /translation="MEHIYRFDTVREYNALNNQETLHPLVSVIDFSKAKPRSWDGRKS
                     VKINYGLYCVFLKEVKCGDIKYGCNYYDYQEGTLVFVAPGQVMEFETDGKVYQPKGYG
                     LVFHPDLIHGTTLGKHMQDYSFFGYQSSEALHLSEKERQIILDCLSKIEFELNQSIDH
                     HSKKLIVSNIELFLNYCNRFYDRQFITRDKAHKGILERFEELLNDYFTTDQPQQTGLP
                     SVAYCAEALHLSANYFGDLIKKETGKSAQEYIQVRIIDRAKHIMTDAGKSISDIAFEL
                     GFKYPQHFTRLFKQKTGMTPNEYRGLN"
     gene            complement(6108..6740)
                     /locus_tag="PLU11_07475"
     CDS             complement(6108..6740)
                     /locus_tag="PLU11_07475"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN58994.1"
                     /translation="MKEFPLILANLEDELSLYLPDPEQVKTVYEQNLLEDQRTPFPYW
                     AKIWASSLALTSYLKKNHGLVNGKRVLEIGAGIGLPSFKFASLAESVLLSDYEPAAVL
                     LMKKNIQHLGLKNVNSIYLDWNQFPEEIKADLVLLSDINYAPEQFESLLLLIKKLLEQ
                     GATIIIATPQRIMAAPFVQELVPFTKYTEVEIIQESGGSVAISILVLSQP"
     gene            6846..7250
                     /locus_tag="PLU11_07480"
     CDS             6846..7250
                     /locus_tag="PLU11_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPN58995.1"
                     /translation="MEAAMNFHTRKWVKPEDLNPNHTLFGGRLLQWIDEEAALYAIIQ
                     LENPHVVTKYISEINFISAPRQGDIIEIGIVATHFGNTSLTMRCEVRNKLTREQLLTI
                     DKLVFVNVDKEGRPAPHGKTKITYVKDRLNED"
     gene            7321..>7973
                     /locus_tag="PLU11_07485"
     CDS             7321..>7973
                     /locus_tag="PLU11_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58996.1"
                     /translation="MNRTLRLAFLLTCILPGFFLIPLSSFSQDYRRWQMNQDGSITWT
                     VNNPEAHDDHIEMSGKQISVVLRYGVGTDGSFHATRSLVWPMLRTIPNNTHASLTRRF
                     AQDGFETVLINGKAINGEKVKNISLNGILQVQSKTDNGFELTREYFPSTDQPLYCEIY
                     TIKNGSAKEAVIELPSTHLEYNTDPVKGTEGGYRIYVDQHGTYIYNVPPGSAIHFSI"
BASE COUNT         2051 a         1849 c         1738 g         2235 t
ORIGIN      
        1 cgtttgagct gcagcaagtg catatccgcc accgccacaa atacctaata aaccgagacg
       61 gtccgcatcc gctcctttat agcgggcgat ataatcagcc ataccccgta tatcctctat
      121 ccggttagcc ggtttgtctg tgttacgggg ctgaccttca ctcgcaccct ggtaagaggc
      181 atcggctgct atgcagatat atccctgttc ggcgagccgt tgtgcgtata aaccagccac
      241 ctgctctttg actccaccat ttggatgagc catcaccagc accgggtatt tctttgtggt
      301 atcaaaacct gcgggtgtat atacattggc cgcaatggta agaccattca gtttataact
      361 gaccgggtga atattcactt tccccggaac attttcttta atggcccctc cgtaaaccag
      421 tccaaatgga tttacggttt tgggttgggc atatataccc gtttgaatag tcataatcag
      481 cagacaggtg aagagttgaa taaattgttt tttcatattg atattcctaa ctgtgtttat
      541 tgatccagtt ttttttcatt cagccatttt gaaagcagtc tggcaatttc aagattgttg
      601 agatcggaga aggggaaatg ggtattccct ttaattcctg cttccggaag gtgaaccacg
      661 gttacatcac cgccatgttt attcaccaca tctctccaca accgggccat ggccagtcgt
      721 acccgccagc catcttcacc cggatgggcg gatggtttgt cgggaatatt atctccataa
      781 taaatgatga taggaatttt ggtgagttgc ataaaatccg caagtggaat agctgaagcg
      841 gtcagtgtac caccggcact tcccatcggt gctggtaact ctccttccgg aaaaagaaaa
      901 ccacttccgg gttcgtatga agcaatggct tttacatttt ggttcttcac cgcagtcagc
      961 cagcccatcc ctcccgaatg agaatgggtg ataagaatag cgggaccggt tttggtaaaa
     1021 agggcagcgg ctgcatcggt gttgacccgg atatcaattg gcccgacatc tggtgtcatg
     1081 gaccggaaat actgattgag cgtattggta tcgcgggcaa actgaacccc ttcgtaataa
     1141 gcgggccaga tacctaagcg aaaagtacca aaccactgtt gttcatcggt agttggatta
     1201 atgttcgcgg ctattgtgct gcggccggca ttgcctcttc gtggctggtc tatcaggtaa
     1261 accgggaacc gttgtctcag gaaaatctgt tgaaatcctt cccggccatc cggagtactt
     1321 tcccatgttt tggaaaactg gccgaatcca tgccacatga tcatgggata tttcctggct
     1381 tttacgggaa tctggtaaaa cacataagca tgatcgccat gtaatgtctg acccgctgca
     1441 gcaggtttgt acggatcgaa cttgccaggg gtggcaatta ccgttccacc tgctgcaaag
     1501 cttccctgtt cccggataac caaaggtttg ttgccggcac tgtttttagt gcctgaacag
     1561 gcaacgagta aaacagcagc tgtaaaaaag gcaaatgatg ttcggacaga ttgattcagg
     1621 ttcttatttt tagccattta ttaaactgat ttaatgtgta aatggttgtt ctggtatggt
     1681 tatttctttt caggtgcttc cacatcagac atatttaata caccttgcgg caggcgaagt
     1741 cctttaattt caatcttatc taagtctgta ttgaaacttg tccattcgtc aacactcagc
     1801 cgtacctggt cggcgccgtt attctccagc atatgtgcca tttgcgtggt acccggaata
     1861 ggtacgatcc agggtttttg tgcaagcagc catgcaaggg atatttgtgc gggggtagcc
     1921 gatttctgct cagcccattt tttaatcaat gctacaaggg caagattgtt cggtaaattt
     1981 tcgggtgtga aacggccttc tcctccacgg atatcgccct gggcaaatct tgtattagcg
     2041 tcaattgctc ccgtaagaaa accaacaccg agcggactcc acggtacaaa gccaattccc
     2101 agttcctcac aaagcggaag gactgccttt tcaggaccac gccacagcag tgaatactca
     2161 ttctggatgg cggtaaccgg gtgagtggca tgtgcccttc tgatggtttg caggcccggt
     2221 tcagagagac catagtgcaa tacttttccc tcgtcgatta agttttttat ggcgccaaca
     2281 atatcttcaa taggtacttc gcgatctacc cggtgctgat ataacagatc aatgcgatca
     2341 gtccgtaagc gtttcagcat accttctacc ttctccttta actgttcagg gcgacttctg
     2401 aaaaattttg tttcgagtac aattttattt cgaaatgggg ctaccgcttc gccaagaatc
     2461 cgttcacttt ccaaaggacc atacacttca gcagtatcaa acagcgtaac gccattttca
     2521 aatgccttac ggataatatt gatcatttcc ggccgggagg gtatcgtggt ttgataggta
     2581 cgcggcatat tttgtacccc cagacctata ctggatactt caagttgctg acccagcctg
     2641 cgtttaccct ttacgatggt aactggtgtt cctgtttttt gggcactggc aaccgatgcc
     2701 agtaaaccgc caccagctac ggccaaacct gctttaaaaa gattgcgacg tttcatatta
     2761 atataatttg aattaatgag atggagtagc agcgggggga gtggccagct ttaccggccg
     2821 catcagcttt gaaaatgtga gtgatcccat cttccattta tttccatctt taatatatac
     2881 ctcagttacc ataaacggat tcaccacttc attgcctcca accaccgcca ccaggtcaat
     2941 ttcatttaac aggatgactg tattaccaat ggtattcacg atcactgagt atacttccgc
     3001 ttttttatac cagatcgtgc cttctttaat cacttttaac tcctgggtct tgccccagct
     3061 accgcccata tgcacgaaca tggatttttc atcgaatagc agattcaagg aatccgtgtt
     3121 cttgtctgcc atccactgcc atttttgttt ggagagccgg gtaatctctt gttccacggc
     3181 tttggagtct gtttgttggg taaaacagat ttgcgaggaa atcaggagta aaaagattcc
     3241 gatgatggtt gatttcatgt tgaatgatta ttagcgttaa tgatatatat gttttgtctg
     3301 aatactatca caaaactcag tgatcctgcc aaacttgttg gtatactggt tactgaaatt
     3361 tctaccatta ttcctgattg gaaatattct ttcggacaat ccgaaaacct gaatattcta
     3421 acataatgcc aattgtatga gaatgaataa tagcataaaa attaagttta ctgtaagtta
     3481 cccgatgtaa cagccttttc aatcagatca agtcttgctt ttttggggtc gggtttgccg
     3541 gttgctttac catccggctg aaactccagt atcccgttat ttttaggatc atataccggc
     3601 cataggggta ggtcctgacc attcgggtca cctgtctttg caaaatttat ccagtaattg
     3661 ttcatcatcc tggctacttc aaggtcattg ggaagtacag gcgaaccatt cacccctctc
     3721 agggtattaa atacatatgg gatttcagat gcatgcgcgg caccaaaccg catccttgat
     3781 tgaaaaggag ctgaaacata ggagaacagg tagatatagg ccttctgctt tttatcggta
     3841 aatgccctgg cggtaaacct ggctggctct gcccagactt tatctgtgtt taccatcgta
     3901 agcagtcggg caaattcggc atctcctcct ggatccagga cggtcgccgc ttcatttttc
     3961 atttttccga ataaagaaaa taaatcctct tttgtccggg cattcacaaa acccgctggc
     4021 acttcagcac tgtttgatcc aataataatg gaaacaccgg cttgtctgcc tgccatatat
     4081 gcagcctgag cggtttcaac gaccaactga ccatcctgta cgggtcccgg ataggtgacg
     4141 ggaccaccct ggccattact ctcctggcca ccatccacga tctgcgcaac tgtaagattt
     4201 ctcagcctgg tcaatgcttc ggcatccgta ccttgaatgc tgtacctttt tgcaaaatta
     4261 ataccgatcg tttctgccga taccggatag taacggtctg cattttcttt acgtattggc
     4321 cggccggtca gcaccccatc acgtccacca cctgactgaa taatagcttt gtgaaaaaga
     4381 ccctttgcgg caggtatact aagtagcgaa tgcactgata cgccacctgc tgattcaccg
     4441 aatattgtaa cattggccgg gtttcctccg aaggcagcta tatttttttg tacccattta
     4501 agagcggcaa tctgatccat gtatccataa ttgccttttg attcttccgg atattcctgg
     4561 cttaaagcgg ggaaggcaaa aaagcctaga cggcccagcc ggtagttaaa tgttataagg
     4621 atcactcctt ttttagcgaa ctgttctcct gaaaaatccg gatgagcgcc gctgccgaaa
     4681 acgaaagctc ctccatgaat ccataccatc acaggtaatt ttgccccttt tttagttccc
     4741 gcaggtgccc agatattaag gaacaggcaa tcctcagaag aagacctgga gatggaatca
     4801 gccccacgtt gaaaaccagc ctgggcacaa tcggcacaga atttatctgc ttcccggatn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atgactgttt ttttcatggt
     4981 aagcatttta gatttgtctg gttccggtaa cagggctgta tttacaggta aaggttgttc
     5041 gtatttccaa cctgaccggt ataccaatta ctgtttttac taccagtttt actgattgac
     5101 ccatccagga gacagagatg ctgtgcaggt gattattttt gtaccttacc ataaaccggg
     5161 aataatggaa catatttaca gatttgatac ggttcgtgaa tacaatgccc tgaataatca
     5221 ggaaacattg cacccgctgg tcagtgtgat tgatttttca aaagcaaagc cccgttcctg
     5281 ggatgggcgt aaatctgtaa agatcaatta cggactgtat tgtgtttttt taaaggaggt
     5341 aaaatgcggg gatatcaagt acggttgtaa ttactacgac taccaggaag gtacactggt
     5401 atttgtagcg cccgggcagg taatggaatt tgaaacagat gggaaagtat accagccaaa
     5461 aggatatggc ctggtgtttc atccggattt gattcatggc actacactcg gaaaacacat
     5521 gcaggattac tccttttttg gttaccagtc cagcgaagcc ctgcacctgt ccgaaaaaga
     5581 aagacagatt atcctggatt gtctttcaaa aattgagttt gagctgaacc agtctattga
     5641 ccatcacagc aagaaactga ttgtttccaa tatcgaactg tttctgaatt actgtaaccg
     5701 gttttatgac cgccagttta tcacccggga taaggcccat aaaggaatac tggaacgatt
     5761 tgaggaactc ctcaatgact atttcaccac ggatcaaccg cagcagaccg gcctgccttc
     5821 cgttgcctac tgcgcagaag ccctgcacct gtccgcgaat tatttcggcg acctcatcaa
     5881 aaaggaaacc ggaaaatccg cacaggaata tatccaggtc aggatcattg acagagcgaa
     5941 acatataatg actgatgccg gaaagtcgat cagtgatatt gcttttgaac tgggctttaa
     6001 atatccccag catttcaccc ggctgtttaa gcagaaaacc gggatgactc ccaatgaata
     6061 ccgggggctg aattaactgg tctaccgaaa atctgaatct gtactattta tggttgactg
     6121 agtaccagaa tgctgattgc cactgatcca ccggactcct gaattatttc cacttcagtg
     6181 tattttgtaa agggcaccag ctcttgtaca aacggcgctg ccattatccg ctgcggggtg
     6241 gcgattataa ttgtggcgcc ttgttccaac agctttttga tcagcaagag caaggattca
     6301 aactgttccg gcgcgtagtt gatatcactt aataaaacaa gatccgcctt aatctcttcc
     6361 gggaattggt tccaatcgag gtatatggag ttgacatttt tcaaacccag gtgctgtata
     6421 ttttttttca tcaacagcac ggcggctggc tcataatcac tgagtagaac tgattctgcc
     6481 aacgaagcga atttaaatga gggaagcccg atgccggccc cgatttccaa tacccgtttt
     6541 ccatttacaa gaccgtggtt ctttttaagg taggacgtta aggccagcga agaggcccag
     6601 atttttgccc agtatgggaa tggggtcctt tgatcctcaa gcaggttttg ttcataaaca
     6661 gtctttacct gttccgggtc gggtaagtag agagatagtt catcctccag gttagccagt
     6721 attaacggaa attccttcat cagattggct gcaagttatt ctttccggcc atcctattcc
     6781 attcaatggg atgaaatttt tcttcggtaa cttgcggctg tccatttcat ttaaatacat
     6841 attgtatgga agctgctatg aattttcata cccgcaaatg ggtaaaacct gaggacctga
     6901 accccaatca tacgctcttt ggcgggcgat tactgcaatg gatcgatgag gaagccgccc
     6961 tgtatgccat catccagctg gaaaatccgc atgtggtgac caagtatatt tcggaaatca
     7021 actttatttc tgccccgcga cagggcgata ttattgaaat cggtattgta gctacccatt
     7081 ttggcaacac ctccctgact atgcgctgtg aagtacgcaa taaactgacc cgggagcaac
     7141 tgctcactat cgacaaactg gtttttgtca acgtggataa agaaggccgg ccggcgccac
     7201 atggcaaaac gaaaatcacc tatgtgaagg acagactgaa tgaagattaa cctgtcgtat
     7261 ttactccggc atgtttttaa agtcattttt taaaatttcc gtaaacgaag caacccgttt
     7321 atgaacagaa ccctccgcct tgcctttcta ttgacttgca ttttaccagg gtttttttta
     7381 attccgctgt cttctttttc gcaggattac cgccgctggc aaatgaatca ggacggatcg
     7441 atcacctgga cggtcaataa cccggaagcg catgatgatc atatcgaaat gagtggcaaa
     7501 cagatctccg ttgtgcttcg ttatggcgtc gggactgatg gctcctttca tgccacccgc
     7561 agccttgtgt ggcccatgct tcgtaccatt cccaataata cccatgccag ccttacccgg
     7621 cgttttgcgc aggacggatt tgaaacggta ttgatcaatg gtaaagcaat caacggggaa
     7681 aaagtaaaaa acatcagcct gaatggaatt ttacaggtgc aaagcaaaac ggacaatgga
     7741 tttgaactga cccgggaata ttttccttcc accgatcagc cgctgtattg cgaaatatat
     7801 acaatcaaaa atggttccgc gaaagaagcg gtgatagaac tgccctccac ccacctggag
     7861 tacaataccg atccggtgaa aggaaccgaa ggcggttatc gtatttatgt ggaccagcac
     7921 ggcacgtata tttataatgt tcctccgggc agtgctattc atttttccat att
//
LOCUS       DAOPMW010000126         7909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10262_length_7909_cov_8.004074, whole genome shotgun sequence.
ACCESSION   DAOPMW010000126 DAOPMW010000000
VERSION     DAOPMW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10262_length_7909_cov_8.004074"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1055
                     /locus_tag="PLU11_07490"
     CDS             261..1055
                     /locus_tag="PLU11_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPN58997.1"
                     /translation="MKKLAPFFLVLVLIACNNGGEEPKPDIPPVKRPNIMSYSVLAAY
                     PHDTSSYTQGLIVYKGQLYEGTGNYGVSRLRRVDLKTGKALQDLALDPKYFGEGITIL
                     RDTIYQLTYKEGKVFVYTLKDFKKIKEYDVEFEGWGLTNDGTNLIVSAGGTNELLYYK
                     PEGFTLLRRQSITEAGMPAENLNELEYIDGFIYANQYQTTYILKINPNSGEVVAKTDI
                     NDLLIRSKSRNNNLDYANGIAYDTATKKVYITGKWWPELYEVQFGQ"
     gene            complement(1056..1952)
                     /locus_tag="PLU11_07495"
     CDS             complement(1056..1952)
                     /locus_tag="PLU11_07495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN58998.1"
                     /translation="MKETIHYSQCPVCGAASIQPVLNAKDYTVSGEVFSICECSVCTA
                     RFTQDIPTAAGIAPYYKSENYISHTNTSKGLINGLYQWVRKRTLKQKRKLVQQQTGVT
                     KGTLLDLGSGTGAFAGEMKNSGWQVTALEPDADARKVAAESFGVTLQDTSEFYNLAAQ
                     QYDAITMWHVLEHVHDLQGYMAKLKTLLKEKGRLIIAVPNYTSGDAAVYGSCWAAYDV
                     PRHLYHFSPAAMKGLVEKHGMKLLSCKPMWYDSFYVSMLSSKYKYGTTRLLSAFINGL
                     RSNISAMSEVKRCSSVIYIIGH"
     gene            complement(2002..2643)
                     /locus_tag="PLU11_07500"
     CDS             complement(2002..2643)
                     /locus_tag="PLU11_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN58999.1"
                     /translation="MYESLYQYFLQYKQLPLPGIGTFFLDRKPAVVNFPDKRMDPPAY
                     AISLQASGVAPSRSFFSWLGVALHVSERDAVIRYNDFAFELKKSILSGAQVNWKGMGQ
                     LSKGLAGDIKFVPVQLIQPEAPVRAEKPIREKAAHKVRVGEDERTSEEMTAFFSKPEE
                     KKNYWWAWALMLGLIAVMYIGWYISTNGVGIAATSNPAVLHAPEAGSTYRIIP"
     gene            complement(2646..4298)
                     /locus_tag="PLU11_07505"
     CDS             complement(2646..4298)
                     /locus_tag="PLU11_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59000.1"
                     /translation="MRQATIKILLSGLGLFCLATASAQKDTTGKGGVTIVSSFKPVLR
                     EAAKINFNATPPAPDTSKARLNYEIPNQNLAFAYQPGSLKPLAMSIDSTVKFDNSSFI
                     KAGFGSLRTPFVQVGITLGDGETAGLNILAKHIASESKRDYQKFSTTNIGLSGYFKSG
                     NNLQWDAGLGMKRDRTYKYGYLPSTLVLSVDSLKQSFQTISGRVAFHNINKTDFGLTY
                     APELKIDVFSDNNKNSESNTVLNAPLQKTIGRNFAVNLGITFDLTRYSPDNKSAINNT
                     MYFISPSVLYKSAQFNVQAGIRPSWDNGKFKMFPNILADISTEDKRFTFHAGWTGYVR
                     KTTYQYLAAQNPWLWIPGELKNTWIEERFAGFKGTVGDHFSYAAKVGFNRLKNQPLFI
                     NDTSAAGDGKSFKVVYEDKVDVLNLGWELGYNVGEKFSLIGGLQFNQFTGLQTQDKAW
                     GMIPLELKAAARMQVIKDLWLKTDLYAFRGPRYMRKDGSDDRHKGAFDLNAGLEFKIT
                     KSINIWTQFNNLFNKEYQRWNQYPVYGFNFVGGVVFSFDQKN"
     gene            complement(4326..7364)
                     /locus_tag="PLU11_07510"
     CDS             complement(4326..7364)
                     /locus_tag="PLU11_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59001.1"
                     /translation="MKNFSLPVLAILFSLSVFSQQTRFYSDPDEKFKEAKEYFQKEKY
                     SLAYPLFRELRQSVKETDKANTAITVQEIYYYTIVCGLKQGEGRSEDEALEYIEVEKN
                     DARVQMMNFHLAEFYYRNENFGEAANRYEQANIANLSNREIADMKFHQGYAYFTLQRF
                     PAAKPLFNTIRQERDDPNYMAANYYYGFLAFKDGQYRDALEAFRVVENEKEYATVVPY
                     YIAQIYYVQGKKEEAITYIQNKLQSGSNTQYYDLELKQMLGHAYFEKKEYGKALPYLA
                     DFVSRSKKVRREDLYELSYSYYQANQLSKAIDGFKQLSGKDDSLSQHAMYLLGDAYLK
                     TGQKSNARNAFLFCSSNSSNIQQKEISKYQYAKLSYELGYQDEALNSLSSFLADYPSS
                     TYTAEAKELLVDVLANTNNYKDAQALLESLAKPSENAKRLYPRILYGRSTELINDGRL
                     DEANAMLDKALKDPYNGSVLPLLNFWKGELAYRNNNIDDAIKYYNAYVSGGSPASGEA
                     NAMNARYNLGYCYLRKENYPVAQTFFEPLGKNPSLSSSSFVQDAYLRTGDCYFMQKEY
                     SKAKSVYDNVVRFSWPAEDYATFQNALLAGVKSSSDKISLLNTMMRKFPASSLVTDAN
                     MEVANTYMGDERFREAVPFLGNVIKNSSNASLIPQAYLKLGTSYYNIDNNTEALKQFK
                     TLVEKYPNSPEAEDALDNVKTIYVEDGKPGEYANFMRQAGRPLSVSTEDSLTYAAAEK
                     QYDDQKINEALNSFNAYLQQFPNGTYALDANFNRAEIYYTRKDWNNALTGYDAVAANA
                     PNKFAERAVLTAARINFFELKTYAKAETYYAQLKQITASQENKLEAMRGLLRCQYQLE
                     KWTEAMDNAKELTAAKGSSADDKSLATMAIAKSYQVAGDYGNAIANFKTVVQINKAAL
                     AAEARYETAACFLQLNKLGDAEKSAFETINKSGSYDYWVTKAYILLGDVYYRQKDYFN
                     AKATFQSIVDNTLNNELKSEAQAKLTKVLDDEAQSSKVGN"
     gene            7626..>7909
                     /locus_tag="PLU11_07515"
     CDS             7626..>7909
                     /locus_tag="PLU11_07515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPN59002.1"
                     /translation="MFTTETKIRVRYAETDQMGVVYHSNFFPYFESARTESIRELGFT
                     YADMEKMGVIMPVVDVHCRYIRPARYDDLLTIRTTLKEMPLQHKIEFHHDV"
BASE COUNT         2123 a         1883 c         1755 g         2148 t
ORIGIN      
        1 aataggtaca gggctcataa aatggccatg agtcattcaa cgggattgga gaaaagtagt
       61 aaggatacag ggaaattgtg cgggcgtctc agggatatga atcggaaaaa gtttcgaaag
      121 acaaggtata aaggtcaaat ataaaatgaa aaatcgtaaa atccaagcac tgtacgtacg
      181 taaaattacc taaccccttc tggtaagctt taactacaaa aaaacatatc cctccttaca
      241 tttgcggtaa taagctatat atgaaaaaat tagccccttt tttcctggta ttagtactga
      301 ttgcgtgtaa taatggtggt gaagaaccaa aaccagatat accgccggtt aagcgcccaa
      361 atatcatgag ctattctgta ttggctgcct atccgcatga taccagttcg tatacccagg
      421 ggctgattgt ctataagggc cagctctatg aagggaccgg aaactatgga gtatccagac
      481 tcagaagagt ggatcttaaa acgggtaaag ccctgcagga tctggcactc gatccgaaat
      541 acttcggaga agggatcacg atactccggg ataccattta ccagttgacc tataaagaag
      601 ggaaagtatt tgtgtacacg ctgaaagatt ttaagaaaat caaggaatac gatgtggaat
      661 ttgaaggctg gggattgacc aatgacggta caaatctgat cgtcagtgca ggagggacca
      721 atgaactgct ttattataaa ccggaaggat ttaccctgct gcgccggcaa tccattactg
      781 aagccggtat gccggctgaa aacctgaatg aactggaata tattgacgga tttatctatg
      841 ccaatcaata ccagaccacc tatatcctga aaataaatcc aaacagtggt gaagtagtgg
      901 ccaaaacgga tatcaacgat ctgttgatca ggagtaaatc acgaaataat aacctggatt
      961 atgccaacgg aattgcatac gacacagcta ccaaaaaagt atacataacg ggcaaatggt
     1021 ggccagagtt atacgaagtt cagtttggcc agtaattaat ggccaataat ataaattacc
     1081 gaactgcatc gcttcacttc actcattgca ctgatattgg aacgcagacc attgataaat
     1141 gcactcaaca ggcgggtggt cccgtattta tatttactgc tcagcatcga cacataaaaa
     1201 ctgtcatacc acatcggttt acagctcagc aatttcattc catgcttttc cactaatcct
     1261 ttcatcgccg caggggaaaa atgataaaga tgcctgggca catcataagc cgcccagcat
     1321 gaaccataca cggccgcgtc gcccgatgta taattgggta ccgcgataat caaccggcct
     1381 ttctctttca acagggtttt cagtttggcc atatagcctt gcagatcgtg tacatgttcc
     1441 agcacatgcc acatggtgat agcatcatac tgctgcgcag ccagattata aaattcactg
     1501 gtgtcctgta aagtcacgcc aaaactttca gccgccactt tacgggcatc ggcatccggc
     1561 tccagggcgg ttacctgcca gccactgttt ttcatttcac cggcaaaagc accggtacca
     1621 ctaccaaggt ccagcagggt acccttcgtg acacccgttt gttgttgtac caacttccgt
     1681 ttttgcttca gggttctttt ccgtacccat tggtaaaggc cattgatcag tcctttggag
     1741 gtattcgtat gggaaatata attctccgac ttataatagg gagcaatacc tgcagcggtc
     1801 ggaatatctt gtgtaaaacg ggccgtacag accgaacact cacaaatgga aaacacttcc
     1861 ccacttacgg tatagtcttt ggcattgagc accggttgaa ttgaagcggc tccgcaaacc
     1921 gggcattggg aataatgaat agtctctttc ataacagaaa gcagcaaagt aaaatcaatt
     1981 tacctaaatg aaagagaaaa cctacgggat aatccggtaa gtagatccgg cttccggtgc
     2041 atgcagcact gccggattgg aggtagcggc tattccgact ccatttgttg aaatatacca
     2101 gccgatatac atgacggcta tcagtcccag catcagcgcc caggcccacc agtaattttt
     2161 cttttcttcg ggcttgctga agaaagccgt catttcctcc gaagtacgtt catcttcacc
     2221 tacccgcact ttatgcgcgg ctttttcccg gataggtttt tccgccctga ccggcgcttc
     2281 gggttgtatt aactggacgg gaacaaactt tatatctccg gccagccctt tactgagctg
     2341 ccccatcccc ttccagttca cctgggcacc ggaaagaatg gattttttta actcaaaagc
     2401 aaaatcatta taccggataa cagcatcccg ctccgaaaca tggagggcta ctcccagcca
     2461 gctgaaaaaa ctgcgggaag gggcaacacc tgaggcctgc aaactaatag cataagcggg
     2521 tggatccatt cttttatccg gaaaattaac cacggcaggc ttcctatcca gaaagaaagt
     2581 accgataccc ggtaatggta attgtttata ctgcaggaaa tattgataga gggattcgta
     2641 cattatcagt tcttttggtc gaacgaaaat acgactccgc ccacaaagtt gaacccataa
     2701 accgggtatt ggttccagcg ctggtattcc ttgttgaaaa gattattgaa ctgggtccat
     2761 atattgatac tctttgtgat cttgaattcc agcccggcat tcaggtcaaa agccccttta
     2821 tgccggtcat cactgccatc tttccgcata tagcgggggc ccctgaatgc ataaaggtct
     2881 gtttttaacc agagatcttt gatcacctgc atccgggccg cggctttcag ttccagtgga
     2941 atcatccccc aggctttgtc ctgagtctga aggcctgtaa actggttaaa ctgcagtccc
     3001 ccgatcaggg aaaacttctc ccccacattg taaccgagct cccagcccag attgagtaca
     3061 tccactttat cttcatacac cactttaaag gatttaccat cgccggcagc tgaagtatca
     3121 ttgataaaga gcggctggtt tttcaaccgg ttgaagccca ctttagcggc ataactgaaa
     3181 tgatcaccca cggtgccctt aaacccggca aagcgctcct ctatccaggt attctttaac
     3241 tcacctggaa tccagagcca gggattctgt gccgcaaggt attgataggt ggtcttcctg
     3301 acatacccgg tccagcccgc atgaaatgta aatcttttgt cttcggtact gatatcggcc
     3361 aggatgttgg gaaacatttt aaacttacca ttatcccagg acggacggat acccgcctgc
     3421 acattgaact gggcggattt atacaatacc gacggagaaa taaaatacat ggtattatta
     3481 atggccgact tattatcggg agaatagcgg gtcagatcaa aagtgattcc gagattcacc
     3541 gcaaagtttc ttccaatggt cttttgcaaa ggtgcattca gcaccgtatt gctttcactg
     3601 tttttattat tatcagaaaa cacgtcaatc ttcaactccg gcgcataggt cagtccaaaa
     3661 tctgttttat tgatattgtg aaaagccacc cggccactga tggtctgaaa actctgtttc
     3721 aaagaatcta ctgacaatac cagggtggaa ggcaggtatc catatttgta agtgcggtca
     3781 cgcttcatgc ccagacccgc atcccattgc agattattac ccgatttaaa ataacccgac
     3841 aacccaatat tagtggtact gaatttctgg taatcccttt tactttctga agcgatatgc
     3901 ttggcgagaa tattcagacc ggccgtttcc ccatcaccca gggtgatgcc gacttgaaca
     3961 aatggggtac gcaggctgcc aaatccggct ttaataaaac tgctgttatc aaacttcact
     4021 gtggaatcaa tactcatggc cagcggcttc agggaaccgg gctgataggc aaacgccagg
     4081 ttctgattcg gaatttcata attcagccgg gctttgctgg tatcgggtgc cggtggggtg
     4141 gcattgaaat taatcttggc agcttcccgg agtacgggct taaagctgga tacaatggtg
     4201 accccaccct tgccggtcgt gtctttctga gcggaggccg tggccaggca aaaaagcccc
     4261 agtccggata aaagtatttt tatagttgct tgtctcatta tgatcgcggt tttcaattca
     4321 gtaaattaat tgcctacttt gctggactga gcttcatcat ccaggacttt ggtcagcttg
     4381 gcctgcgctt ctgattttaa ttcgttattc agggtattat ccacgatact ctggaaagtg
     4441 gctttcgcat tgaagtagtc cttctgccgg tagtatacat cgccgagcag gatataggcc
     4501 ttggtcaccc agtaatcgta agaaccggac ttgttgatgg tttcaaaagc tgatttctct
     4561 gcatccccca gcttattcaa ttgtaaaaaa caagccgctg tttcatacct ggcttcagca
     4621 gccagcgccg ctttattgat ctgcaccacc gttttgaaat tcgcaatcgc attaccataa
     4681 tctccggcta cctggtagga tttggcaatg gccatagtgg cgagtgattt atcatcggca
     4741 ctgctgccct tggcggccgt gagttccttt gcattatcca tggcttccgt ccatttctcc
     4801 agctggtact ggcaacggag caggccacgc atggcttcca gcttattctc ctggctggcg
     4861 gtgatttgtt tcagttgtgc gtaatacgtc tcagcctttg cataagtttt caattcaaag
     4921 aagttaatcc gggccgccgt caataccgct ctctcggcga acttattagg cgcattagcc
     4981 gccaccgcat catagccagt caatgcattg ttccagtctt tacgcgtata atatatctcc
     5041 gcccggttaa agttggcatc cagcgcatag gtgccattgg ggaactgctg caggtaagca
     5101 ttaaaactat tcagggcttc attgattttc tggtcatcat actgcttttc agccgcggca
     5161 taggtcagcg aatcttccgt actcacactc agcggacgac cagcctgccg catgaagttg
     5221 gcatactcac cgggcttgcc atcttccaca taaatcgtct tcacattatc cagcgcatcc
     5281 tcagcctccg gcgaattggg atatttttcc accagggtct taaattgttt cagtgcttcg
     5341 gtattattat caatattata ataggaggtt cccaatttca ggtaggcctg gggaatcagg
     5401 ctggcattac tgctgttttt gatcacatta cccaggaaag gcacggcttc acggaaacgc
     5461 tcatccccca tatacgtatt ggccacttcc atattggcat cggtaacgag cgaagaagcg
     5521 gggaatttac gcatcatggt attcagcagg gatattttat ccgaagaact tttcaccccc
     5581 gccaacaaag cattctggaa agtggcatag tcttcggccg gccaggaaaa ccgtactaca
     5641 ttatcataca ccgatttggc cttgctgtat tctttttgca taaaatagca atctcctgtc
     5701 cgcaggtaag catcctgtac aaaagaagat gaactgaggg atggattctt tcccaaaggc
     5761 tcaaagaaag tttgtgccac cgggtagttc tccttgcgca ggtaacaata cccgagatta
     5821 tagcgggcat tcatggcatt ggcttccccg ctcgcaggag aacctccgct aacatacgca
     5881 ttgtaatatt ttatcgcgtc atcaatatta ttgttccggt aagccagttc ccctttccag
     5941 aaattaagca aaggtaatac cgacccgttg tacggatctt tcagggcttt atccagcatg
     6001 gcattcgctt cgtccagccg gccgtcattg atcagctccg tggaacggcc ataaagaatc
     6061 cggggataaa gccgtttggc attctccgac ggtttggcca gactctccaa cagggcctgc
     6121 gcatctttgt aattattggt attggccagc acatccacca gcagttcttt ggcttccgcc
     6181 gtataagttg aactgggata atcagccaga aaggaactca ggctgttcag ggcttcatcc
     6241 tggtaaccga gctcataaga gagtttagca tattgatact tggaaatttc tttttgctgg
     6301 atattactgc tattggacga acagaagaga aaggcattcc gggcattgga cttctggccg
     6361 gttttgaggt aggcatctcc taacaggtac atggcgtgct gactcagtga atcgtctttt
     6421 ccacttaact gcttaaatcc atcaatggct ttactgagct gattagcctg gtaatagcta
     6481 taggaaagtt catacaggtc ttcacgacgg actttttttg accggctgac aaaatcagcg
     6541 aggtatggaa gggctttccc atattccttc ttttcaaaat aagcatgacc cagcatctgt
     6601 tttaattcaa ggtcgtaata ctgggtatta ctgccagact gcagtttatt ctgaatataa
     6661 gtaatggctt cttctttttt tccctgcaca taatagatct gtgcaatata ataaggcact
     6721 acagtcgcat actctttttc attttccact acccggaacg cttccagggc atcccggtac
     6781 tggccatctt taaaggccag aaatccgtag taatagtttg ccgccatata attgggatca
     6841 tcccgttcct gtcggatggt attgaacagg ggtttggcag ccgggaaccg ttgcagggta
     6901 aaataggcat aaccctggtg aaacttcata tccgcgatct cccggttact caggttggca
     6961 atattagcct gttcgtagcg gttggccgct tccccaaagt tttcattgcg gtaataaaat
     7021 tctgccaggt gaaaattcat catctgcacc cgggcatcat ttttttccac ttcaatatac
     7081 tccagggcct cgtcttctga acgaccttcg ccttgcttga gaccacaaac aatcgtataa
     7141 taatagatct cctgtacggt aatggccgta ttggctttat cggtttcctt caccgactgg
     7201 cgcagttccc ggaacaaagg ataggccagg ctgtatttct ctttttgaaa atactccttg
     7261 gcttctttga atttctcgtc gggatctgaa taaaaacgcg tctgctggga gaaaacggac
     7321 agactgaata agatggctag tacaggtaaa ctgaagttct tcattgattt gtcctttaac
     7381 cggtggaggt ttaacgttgc gaatattcaa cttatttcaa gggagaccaa acatcattcc
     7441 aaacaggtgt ggataagtca gcaattgtgc atgctttaac attttcttgc tgagacgggg
     7501 tgtttttcgt gtaaaaaacg gttggaatca tacaaacaaa aaaagagggc atcggaactt
     7561 tttatcgtat accctgttcc ggaactgctt tctatcaaaa aaggaaatag tgttagtttc
     7621 gcagcatgtt tacaactgaa accaaaatca gggtacgata tgccgaaaca gaccagatgg
     7681 gtgtcgtata tcatagcaat ttcttcccct attttgaatc cgcccggaca gaatcgatcc
     7741 gggaactggg gtttacttac gcggatatgg aaaaaatggg agtgattatg ccggtggtgg
     7801 atgtgcattg ccggtatatc cggccggccc gctatgacga tttgctgacc atcagaacaa
     7861 cactgaaaga aatgcccctg caacacaaga tcgaattcca ccatgatgt
//
LOCUS       DAOPMW010000127         7888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10302_length_7888_cov_6.637687, whole genome shotgun sequence.
ACCESSION   DAOPMW010000127 DAOPMW010000000
VERSION     DAOPMW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10302_length_7888_cov_6.637687"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1187)
                     /locus_tag="PLU11_07520"
     CDS             complement(123..1187)
                     /locus_tag="PLU11_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN59003.1"
                     /translation="MLKFILRKMAYGLLVLLGVIALVFFLFQGFGDPSRIVMGQTGDS
                     TTQANIRKDLYLIDEQGTPIPKFKQFLYYLNDVSPVCIHSNTDIEKKKLRGFFTGGEK
                     KFGIKIPYLRKSYQSKREVWTILVQALPGTLMLALAAMAIAITIGIPLGVVAAVKQNS
                     WIDTSAIFSSIVGISAPSFFMGIIMVYVFGFVLSEWTGLHIAGSWFAIDDEGQRRLSL
                     QNLILPAVTLGIRPLAIITQLTRSAMLDVLDQDYIRTAYAKGLSRRTVIWKHGLRNAL
                     NPVITAITGWFAELLAGAFFVEYIFGWQGIGKITVDALEKLDYPVVMGSVLISAGFFI
                     FINILADILYGVVDPRVRLS"
     gene            complement(1193..2359)
                     /locus_tag="PLU11_07525"
     CDS             complement(1193..2359)
                     /locus_tag="PLU11_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPN59004.1"
                     /translation="MKALVTFVRLLVGVLFIFSGLVKANDPLGLSYKMQEFFEVWQLH
                     GWNETTLALSIFMNAFEIIAGFALLIGWQFRLVSWLLLLLIVFFTFLTGYTYITGMPK
                     NCGCFGDCLPITSLTSFLKDVVLIVLILFLLLNRKMVRPLLPAKMALLSMSLVIIFSF
                     GFQYYTLQHLPVVDCLPFKKGNNIIEKRQVPADAIPDSFAIRFIYSKGGKEFEFSQLE
                     LPADLNTYKFISRKDKLIRKGNAEAAIKGFDLKGQSGMDSTLQVLQQPIAVLLIGEDL
                     KHPSVSWTAGFSSVFNTSKQKNIPVYIITGQAGNAIQITGGTAYASVPVFTCDNVTIR
                     TAARSNPTLYIIQQGTITGKWSGSDLGKAEKVLTELPDMPPPAPVQQQPTDSIQ"
     gene            complement(2359..2889)
                     /locus_tag="PLU11_07530"
     CDS             complement(2359..2889)
                     /locus_tag="PLU11_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HPN59005.1"
                     /translation="MAITNQQYDQVISGCREIFIKKATDYGTAWRVLRTISIVDQIFI
                     KAQRIRTIQEKGAQKVSDGIAAEFKGIINYCVIGLIQLELPADAPEELAIGEVAALYE
                     QKVQTAKALMQDKNHDYGEAWRSMSQESFTDLILMKLQRIRQILSNDGKTLISEGIDA
                     NYLDILNYAVFALILI"
     gene            3006..>3322
                     /locus_tag="PLU11_07535"
     CDS             3006..>3322
                     /locus_tag="PLU11_07535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPN59006.1"
                     /translation="MFSLNCKGRLLVVDKPLVMGILNITPDSFYSGSRTQTVDEVLFR
                     AEQMLMEGAAILDLGGQSTRPGSEQLNADNELSRVIAPLEALHKRFPEAYISIDTYYA
                     KVAR"
     assembly_gap    3323..3422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3423..4000
                     /gene="folP"
                     /locus_tag="PLU11_07540"
     CDS             <3423..4000
                     /gene="folP"
                     /locus_tag="PLU11_07540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPN59007.1"
                     /translation="RFPEAYISIDTYYAKVARYAVEAGACIVNDISGGSLDPQLIPEV
                     AALKVPYVLMHIKGNPQTMQQQAVYADVTREVLDQLADRKAVIEKAGIRDCIIDPGFG
                     FGKTAAHNFRLMEELPVFSLFKAPVLLGISRKSFIWKTLGTTPESDDTLQGTTALHMA
                     GLIKGANLLRVHDVKAAAATIKLFLEMKGAG"
     gene            complement(4125..5054)
                     /locus_tag="PLU11_07545"
     CDS             complement(4125..5054)
                     /locus_tag="PLU11_07545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPN59008.1"
                     /translation="MNKMKPLFLLLITGTLLVSCDKAAYRKTPGGMPYQLFAGKDTTS
                     VKVGNFLKLEFTQKINDSTYFSTVGSLPLYIEVNGQSNPYDVTELWTLLHDGDSVVAT
                     QMMDTFIKRAPPGQMNPKFKKGDRVMTYVKVLAVYANDSIKTVAENKDRDAFTAREAK
                     VVEDFVKSKKATVQRTPSGAYVEILEPGTGNLIDSGKYISVNYTGTTFKGEKFDSNID
                     PAFGHAQPMSFTVGSPGMSKGFDESMLSLRNGASARLYVPSMLAYGGNPPRGSKIKPF
                     ENLMFEVKVIDVKDQAPPPPANPAPDQPQIKLQ"
     gene            complement(5068..6075)
                     /locus_tag="PLU11_07550"
     CDS             complement(5068..6075)
                     /locus_tag="PLU11_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPN59009.1"
                     /translation="MQPIQDIFPLLNQPRRIVITTHQKPDADAMGSSLALRHFLVQLG
                     HTVTVISPTNWAGWVDWMPGTREVMDYERDRQKADAILDQADWLFCLDFNIFHRTKSL
                     TEKLKQLTCTKILIDHHQEPDIPSFNYGISDTSKSSTCEMIYDFIVDSGNRDKINEEI
                     GECIYAGVVGDTGSFRFSSAHAGVHTLVADLKTRGLNHGKIHEGLFDNFLENRLRFIG
                     HVLLHRMEILYEYNAALIAIPKNDILKFHIRTGDTEGLVNFPQSIQGIKLVALVIDRD
                     EERKWSFRSKGQFDCNTFARNYFEGGGHYNAAGGRSSESIEKTVERFKAVLKENATLL
                     Q"
     gene            6258..6674
                     /locus_tag="PLU11_07555"
     CDS             6258..6674
                     /locus_tag="PLU11_07555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPN59010.1"
                     /translation="MSNRTFTMIKPDAMKNGHAGAIIDRIIKEGYRVVALKQTKLSAE
                     KAGEFYAVHKERPFYGELVEFMSSGVIIAAILEKENAVASFRELIGATNPAQAAEGTI
                     RKLYATSLGENAVHGSDSDENAKIEGAFFFSGLEQF"
     gene            complement(6862..7308)
                     /locus_tag="PLU11_07560"
     CDS             complement(6862..7308)
                     /locus_tag="PLU11_07560"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HPN59011.1"
                     /translation="MRNFFIVFIILLGSLTLIGAGKPLAEEGIRITVTQLHNEKGHVL
                     VSLFKDGTGYPDDANKAFRKIKLDISGKKATVLFTGLPAGNYAVSILHDENDDQKMNK
                     TALGIPKEGFGFSNNVVGAFGPPSWSRASFAHKPGVLTLREIRARY"
     gene            complement(7376..>7888)
                     /locus_tag="PLU11_07565"
     CDS             complement(7376..>7888)
                     /locus_tag="PLU11_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPN59012.1"
                     /translation="MMASSLLSEGDLPCEGATSMEQIIMGAAKQQGKKIKGLETMAQQ
                     AGFLDSIPYKYQAAELVKYIDSAGTDNGQSTMMDQLFDAYRNQDLKKLEDLMLDSDAG
                     MSSFSDILLYRRNANWVMKLKTLMTDKSLLVAVGAGHLPGEKGVISLLRKAGYTVTPV
                     ENKRIRTKEI"
BASE COUNT         2084 a         1783 c         1794 g         2127 t
ORIGIN      
        1 ctgatttaat ttttccatgg cttaccgttt taatgaataa gactgaaaag aaccgatacc
       61 agtaatttac tacaactacc gtactaaaaa catgaccttc attaaatgtg acgaaaaaat
      121 gttcagctga gcctgacccg cggatccacc accccataaa ggatgtcggc caggatattg
      181 ataaaaataa aaaagccggc tgagatcaaa acggatccca ttactaccgg gtaatccagt
      241 ttctccaatg catccacggt tattttccca atcccttgcc acccaaagat atattccaca
      301 aaaaaagcac cggcgagcaa ttcggcaaac cagccggtaa tcgctgtaat gaccggatta
      361 agtgcattcc ggagtccgtg tttccagatc actgtccgcc ggctgagtcc cttggcatat
      421 gcagtacgga tataatcctg atccagtaca tcgagcatgg cactacgggt tagctgggta
      481 atgatggcta atggtctgat cccgagtgtg accgccggca ggatcaggtt ttgcaacgag
      541 agtcgtcgct gtccttcatc atcaatggca aaccagcttc cggcaatatg cagcccggtc
      601 cattcactca gcacaaagcc aaatacatag accatgataa tgcccatgaa aaaagagggt
      661 gcagatatgc ccacgatgct gctgaagatc gcagaggtat cgatccagct gttttgtttt
      721 acagcggcca ctactcccaa cggtatcccg atggtaattg caatcgccat tgcagctaaa
      781 gccagcatca gtgtgccggg taatgcctgt accaggatgg tccagacctc ccgcttgctc
      841 tgataagatt tgcggagata tggaatttta atgccgaatt ttttttcacc cccggtaaag
      901 aagccccgca gttttttctt ttcaatatcc gtgttgctat gaatgcatac cggtgaaaca
      961 tcattgagat aatagagaaa ttgtttgaat ttcgggatgg gtgttccctg ttcatctatg
     1021 agataaagat ccttccggat attagcctgc gtggtggaat cgccggtttg tcccataaca
     1081 atcctggacg ggtcgccaaa accctgaaaa aggaaaaaga ccaatgcgat cacccccagt
     1141 aaaacaagga ggccgtaggc catcttacgg aggatgaatt tcagcatact ttctattgaa
     1201 tactatctgt aggttgttgt tgaaccggtg ccggtggcgg catatccggt aattctgtaa
     1261 gtactttttc cgctttcccc aaatcgcttc cgctccattt tccggtgatt gtaccctgct
     1321 gaataatata caaggttgga ttggagcggg cagcggtacg tatggtaaca ttatcgcagg
     1381 taaagaccgg aactgaagca taggcagttc cacctgtgat ttgaattgca ttaccggcct
     1441 gtccggtgat aatatagacc ggaatatttt tttgtttact tgtattaaaa acagacgaaa
     1501 aaccagctgt ccaggaaacg gaaggatgct tcaggtcttc gccgatcagt aaaacggcaa
     1561 ttggctgttg caggacctgc agggtggaat ccataccgga ttgtcctttc aggtcaaatc
     1621 ccttaatggc agcttctgca ttgcctttac ggatcagttt gtctttccgg ctgataaatt
     1681 tataggtatt cagatctgca ggcaattcga gttgggaaaa ttcaaattct ttacctcctt
     1741 tgctgtaaat aaaacggatg gcaaaactat cgggaatcgc atcggccgga acttgtcttt
     1801 tctcaatgat attatttcct tttttaaacg gaaggcaatc caccaccggc aggtgctgca
     1861 gggtataata ttgaaatcca aagctgaaaa tgataaccag ggacatggat aatagcgcca
     1921 ttttagcagg cagtaatggc ctgaccattt ttctatttag caacaggaaa agtatcaaca
     1981 cgatcagtac cacatctttt aaaaacgatg tcagcgaagt aatgggcaaa caatccccga
     2041 aacaaccaca gtttttaggc ataccggtta tgtacgtata gcctgtaagg aaggtaaaga
     2101 aaacgatcag taataaaagc aaccagctca ccaggcggaa ttgccagccg atcagcagtg
     2161 caaagcccgc aatgatctcg aaggcattca tgaaaatgga taaagccagt gtggtttcat
     2221 tccatccatg tagctgccac acttcaaaaa attcctgcat tttataactc agtccgagcg
     2281 ggtcgttggc tttgaccagt ccggaaaaga taaaaaggac gccgaccaac agccggacaa
     2341 aggtgacaag tgctttcatc agattaatat tagtgcaaaa acggcataat tcaatatatc
     2401 gaggtaattg gcatcaatgc cttcgctgat cagtgttttg ccgtcattgc tcagaatctg
     2461 acggatacgt tggagtttca tcaaaatgag gtcggtaaaa ctctcctggc tcatgctgcg
     2521 ccaggcttct ccgtaatcat gattcttatc ctgcatcagg gctttggcgg tttgtacttt
     2581 ctgttcgtat aaagcggcca cttctccaat cgccagttct tccggtgcat ctgcaggcaa
     2641 ttccagttga atcagtccga tgacacaata gttgatgatg cctttgaatt cagccgcaat
     2701 cccgtcagaa actttttgtg ctcctttttc ctgtatggtc cgtatccgtt gcgccttgat
     2761 aaagatctgg tccacgatcg aaatggtacg aagcacccgc caggccgtgc catagtcggt
     2821 ggcttttttt ataaaaattt cccggcagcc gctgatcacc tgatcgtatt gttgattcgt
     2881 aatagccata agtcagaaaa tagaaaatag agaatagtaa atagggagag aaagccggga
     2941 aaaatgaatt tccgttggtt agccgctctc tgttatctta gcttcaaaat taacaaacaa
     3001 gactgatgtt ttcgctcaac tgtaaaggaa gattactggt tgtggataag ccgctggtga
     3061 tgggtatact caatataaca cccgattcct tttacagcgg cagccgtacc cagacggtgg
     3121 atgaagtgct tttccgggcc gaacaaatgc tgatggaagg tgctgccata ttggatctag
     3181 gcggccagag tacgcgtccg ggcagtgaac agctgaatgc agacaacgag ttaagccggg
     3241 tgatcgcccc gctggaagcc ctgcacaaac gttttccgga ggcttatatt tctatcgaca
     3301 cctattatgc aaaagtggcc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnaacgtttt ccggaggctt atatttctat cgacacctat tatgcaaaag tggcccgtta
     3481 tgctgtggaa gccggtgcct gcatagtcaa cgatatcagt ggcggcagtc ttgatcctca
     3541 attaatccct gaggtagctg cactgaaagt gccatatgtg cttatgcaca ttaaaggcaa
     3601 tccgcaaacg atgcaacagc aggcagttta tgcggatgtc acaagggaag tgctggatca
     3661 gctggcggac cggaaagcag tcattgagaa agccggtatc cgggattgta tcattgatcc
     3721 gggttttggt tttggaaaaa cggctgccca taatttcagg ttgatggagg aactccctgt
     3781 gttttcactc ttcaaagccc cggtcctgtt gggcatttcc cggaaatctt ttatatggaa
     3841 gaccctgggt actacaccgg agtctgatga tacgttgcag ggtactaccg ccttgcatat
     3901 ggccggactg ataaagggcg ctaacctgtt aagggtgcat gacgttaaag cagccgccgc
     3961 tacgattaag ttattccttg aaatgaaagg ggccggttaa aaagcggtgt ttgactaaac
     4021 tgatattggg taaccaggaa ttataaaaaa agtacccgtc ctttacagcg acgggtacaa
     4081 acttgcaccg ttttaaatac aggtgcggaa ttattttaag tagcttattg caacttgatc
     4141 tgtggttgat ccggtgccgg gtttgccggt ggcggtggtg cctgatcttt cacgtcaatc
     4201 actttcactt caaacatgag attctcgaaa ggcttgatct ttgaaccacg gggcgggttc
     4261 ccgccatatg ccagcatgga cggaacgtac aggcgggctg aagcaccatt gcggagtgaa
     4321 agcatagact catcaaatcc tttgctcata ccaggcgatc ccacggtaaa agacatgggt
     4381 tgagcatgac caaatgcggg atcgatattg ctatcaaatt tctcaccttt gaatgtggtg
     4441 ccggtataat tcactgatat gtattttccg gagtcgatca ggttaccggt accgggttca
     4501 aggatttcta cataggcacc tgacggtgta cgttgtacag tcgctttttt ggacttgaca
     4561 aaatcttcca ccactttggc ttccctggca gtaaatgcgt cgcggtcttt gttttctgcg
     4621 acggttttga tactgtcatt tgcatagaca gccagcactt ttacataggt cattacacga
     4681 tcccctttct tgaatttagg attcatttgg ccggggggag cgcgtttgat aaacgtatcc
     4741 atcatttggg tagctaccac actgtcgccg tcatgcagta aggtccagag ttcagtcaca
     4801 tcgtaaggat tactctgtcc atttacctca atataaagtg gcagagatcc tactgtggaa
     4861 aaataggtac tgtcattaat tttctgggta aattccagtt tcaaaaaatt gccgactttt
     4921 actgaagtgg tatccttacc tgcaaaaagc tggtagggca tgccacccgg tgtttttcgg
     4981 taagcagctt tatcacagga aaccagcagg gttccggtga tcagcagcaa aaacaatggt
     5041 ttcatcttgt tcatacttgg gttttaatta ttgtaaaagt gttgcatttt ctttcaggac
     5101 cgctttgaag cgttccactg tcttttcaat ggattcactg ctacggcctc cggcggcatt
     5161 atagtgacca ccaccttcga aatagttgcg ggcaaaggta ttacaatcaa actgaccttt
     5221 gctgcgaaaa ctccattttc gttcctcgtc acggtcaatg accagggcta ccagcttgat
     5281 gccctggatg gattggggaa aattaaccag cccttccgta tcgccggtcc ggatatgaaa
     5341 cttgagaatg tcatttttgg gaatggcgat cagtgctgca ttgtattcgt agagaatctc
     5401 catccggtga agtaatacat gcccgataaa gcgcagcctg ttttccagga aattatcaaa
     5461 taatccttcg tggatcttac catgattcag tccccttgtt ttcagatccg ccaccagggt
     5521 atgtaccccg gcatgggctg atgagaaacg gaaggaaccg gtatccccta ccacacctgc
     5581 ataaatacac tccccgattt cttcattgat tttatccctg tttccggaat ccacgataaa
     5641 atcatagatc atttcacagg tggaactttt tgatgtatcg ctgatgccat agttaaatga
     5701 cggaatatca ggctcctgat gatggtcaat taaaatttta gtgcaggtta attgttttag
     5761 cttttcggtg agggatttag tccggtggaa aatattgaaa tccaggcaaa acagccaatc
     5821 ggcctgatca aggatcgcat ctgctttctg ccggtctctc tcatagtcca tcacttcccg
     5881 ggtaccgggc atccaatcca cccagccagc ccagttggtg ggggaaataa cggttacagt
     5941 atggccgagc tggacaagaa aatgacgcag ggccaaggat gagcccatcg catccgcatc
     6001 cggcttctga tgggtggtaa taacgatccg tcggggctga tttaataagg gaaatatatc
     6061 ctgtataggt tgcataagaa gtgtgcgaag gtaggatttt tagttcgtag ttagtagtta
     6121 gaagttggta gtcttgtact cccggacttt cccggattta cgagattttc aggttcttca
     6181 ggtgaatcag actactaact tctaactact aactaccgac tattttccct acctttgcgg
     6241 cgcaaaaaca acccaatatg agtaacagaa cttttaccat gatcaagccg gatgcaatga
     6301 aaaatggcca tgccggagca atcatcgatc gcattattaa agaagggtat cgtgtagtgg
     6361 ccctgaaaca gaccaaactg agtgcagaaa aagccggcga attctatgcc gtacataaag
     6421 aaagaccttt ttacggggaa ctggtagaat tcatgagcag cggggttatt attgccgcaa
     6481 tcctggaaaa ggaaaatgcc gtggcttctt tccgtgaact gattggtgcc accaacccgg
     6541 cacaggctgc agaaggaacc atccgtaaat tatacgccac ttctttaggt gaaaatgccg
     6601 tacacggcag cgacagcgat gagaatgcaa agattgaagg agcttttttc ttcagcggac
     6661 tggagcaatt ctgagtcagt tagtagttag cagatagtag ttagtagtcg ctacacacgg
     6721 acaatacttt ataaaagggg atttatcatt ctttgagata tccccttttc ctatatatat
     6781 agatgcttaa ttaaacttca tcagtcggca gtcggcggca ggcaacttgt aactcgtacc
     6841 tcatacctcg tagcttgtat tctaatatct cgccctgatc tcacgcaacg taagtacccc
     6901 gggtttgtgt gcgaaactgg ccctgctcca ggagggcgga ccaaaagccc ctactacatt
     6961 attggagaaa ccaaaacctt ctttgggaat accgagggcg gttttattca ttttctgatc
     7021 atcgttttca tcatgcagga tggataccgc ataatttccg gcgggcaaac cggtaaataa
     7081 gaccgtcgcc tttttcccgc tgatatcgag tttgattttc cggaatgctt tgttggcatc
     7141 atcaggatag cctgtaccat ctttgaacaa actgacaagt acatgccctt tttcattatg
     7201 aagttgggtg accgtgatcc ggatgccctc ttccgctaac ggtttaccgg caccaatgag
     7261 ggtcaggctt cccaacagaa taataaatac aatgaaaaaa ttacgcatga aatgtataca
     7321 aatcctgact cgtatggttc agtaaaaggt aaaatcgttt cctgaaaaag atagatcaga
     7381 tttccttcgt cctgatccgt ttattttcta caggtgttac ggtataaccg gctttacgga
     7441 gcagactgat cactcctttt tctccgggca ggtgtccggc ccctacggct accagcaagg
     7501 atttgtctgt catcagggtt tttaatttca ttacccagtt ggcattgcgc cggtaaagca
     7561 ggatatccga aaaactactc atccccgcat cactgtccag catcaggtct tccagttttt
     7621 tcaggtcctg gttccggtag gcgtcaaaaa gctggtccat catcgtgctt tgcccgttat
     7681 ccgttccggc actgtcaata tatttcacca gttctgctgc ctggtatttg tatggaatac
     7741 tgtccaggaa accggcctgc tgagccatcg tttccagacc ttttattttt ttgccctgct
     7801 gctttgccgc tcccataatg atctgttcca tggaagtagc tccttcacat ggcagatcgc
     7861 cttcagataa cagggaagac gccatcat
//
LOCUS       DAOPMW010000128         7767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10537_length_7767_cov_7.571706, whole genome shotgun sequence.
ACCESSION   DAOPMW010000128 DAOPMW010000000
VERSION     DAOPMW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10537_length_7767_cov_7.571706"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PLU11_07570"
     CDS             complement(<1..1591)
                     /locus_tag="PLU11_07570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN59013.1"
                     /translation="MNKFQELGIEEGLLLSIETLGFTQPTPIQEKAIPVLLQGTKDFI
                     GLAQTGTGKTAAFGLPLLQLIKKEDRFPQALIVCPTRELCLQITSDLEKFKNKKVSVS
                     VTAVYGGTSISMQIRDLKRGTHIVVATPGRLIDLIERKAINLEQIHYVVLDEADEMLN
                     MGFKEDIEFILKNTPNRQSTWLFSATMPAEIRQVSKRYMDKPFEITIGKMNEGNANID
                     HHYYMTQHHNRYEVLKRIIDFNPGIYGIVFARTKADTQDIAEKLTREGYDIDAIHGDL
                     TQQQRDKVMGLFRDRSVRLLAATDVAARGIDVQGITHVINFELPDDVEVYTHRSGRTG
                     RAGNSGVSVSIVTPKEVYRLRQIEKLVNTRVHKMDIPSGKDVCRKQFFSFIDKMLGAD
                     ISHGEYETYLPILREKFAHVEKEEILQRVAALEFDRFLKYYENAADLNLRDAGKKTER
                     GERGAARFEQRDTQGRKFGGGGGSYQRLFVNLGTKDGFYKASFLQFILDMSDLKKDVL
                     GRIDMKEMNSWVEIEAGAAKKM"
     gene            complement(1757..1969)
                     /locus_tag="PLU11_07575"
     CDS             complement(1757..1969)
                     /locus_tag="PLU11_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59014.1"
                     /translation="MKKFLAIAAIALFAVACNNSTESKDATADSLAKVATADSLAKVA
                     ADSAAKAMDTAAKVPADTAAKKDSAK"
     gene            2109..2684
                     /locus_tag="PLU11_07580"
     CDS             2109..2684
                     /locus_tag="PLU11_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN59015.1"
                     /translation="MKQAINEKELLQGLAVNDKKAVETIYKENYNMVQSLIINNNGSA
                     DDAKDIFQEAMIVLYEKVRSGSFELNCLIKTYVYSVSKRLWLKRLQQLNRYSPPIDDL
                     ESPVPVDEDMEDHERRDAEFEMMNKAISSLGEPCKSLLEAYYLQKQNMQVIAANFGYT
                     NADNAKNQKYKCLMRLKKIFFSHYKNENGNG"
     gene            2677..3792
                     /locus_tag="PLU11_07585"
     CDS             2677..3792
                     /locus_tag="PLU11_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPN59016.1"
                     /translation="MGDTKVLEAVERYIRGEMNPDERLHFENLRKSNGEIDELVVEHT
                     LFMQQMNRFGEWKKFRSTLHDVHTDLAEQGKINSARLTGKAKVVYLWNRYKRVAAIAA
                     SIAGITTLTISALVWTITPQTAPGELQQLKGKITIIENTTKAQGQEINKVKNKISSSD
                     NTIVYTTGGTGFIIDTKGYLVTNVHVVEGAKNIAVQNSRGEYTAKVVYIDAAKDIAIL
                     KIDDESFKPYSSIPYTISRSSGKLAEPIFTLGYPRNEVVYGQGYLSSKTGYKGDTLSC
                     QLELTANQGNSGSPILNKKGEVIGILNGRQKNAEGFSFAIQGKHIYKVLEDLKKDQKK
                     DTSYQRIKLNSRSSISGMDFQQQVEKVEDYVFMVKVN"
     gene            complement(3869..4762)
                     /locus_tag="PLU11_07590"
     CDS             complement(3869..4762)
                     /locus_tag="PLU11_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPN59017.1"
                     /translation="MKPTLVILAAGMASRYGSMKQVQGFGPNGETIMDYSIYDAIRAG
                     FGKVVFIIRKEFADNFKTIFEPKLNGKVQTEYVFQEMDYYTEGYKAAAERTKPWGTAH
                     AVLCAKEVVKEPFAVINADDFYGSNAFENAASFLNNGCNENVYAIVGYDLLRTLSDHG
                     TVNRGVCGLDADGNLATVTERINIAKKEGKILCEDGLEPKELSLETRVSMNFWCFDPS
                     FFAYTQKLFIEFLHASGQELKSEFFIPIVADQFIKEGGKVEIIPTTSNWFGVTYKEDA
                     PFVQNSLNTLIEKGEYPAKLW"
     gene            4820..5710
                     /locus_tag="PLU11_07595"
     CDS             4820..5710
                     /locus_tag="PLU11_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPN59018.1"
                     /translation="MQYINPEHIRVDPYLSPLFDDKEITANILRLDLLHPVVSGNKWF
                     KLRYYIEKAQEQNKNSLVTFGGAWSNHIHATAAAGKMVGLKTIGIIRGEAPARLSTTL
                     LQATELGMQLHFISREEYRQKPIPESIDLSSSQLIPEGGYGTTGARGAATILSLINPD
                     DYTHIVCAVGSGTMMAGLMNAYWSKGVVLGISAMKNNQALESDVRQLLEVPAISPVII
                     HDYHFGGFAKYNSRLLDFMNEVYRYTGIPTDIVYTGKLFFALNDLAWKNYFPPGSKIL
                     LIHSGGLQGNQSLPNGSLIF"
     gene            5782..6645
                     /locus_tag="PLU11_07600"
     CDS             5782..6645
                     /locus_tag="PLU11_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59019.1"
                     /translation="MKKGSLYFFLLLAVCSVSNLFAQDTLPKFSLKNVGKNRIVVSWT
                     NPFPTIGQLSIQRSYDSIKGYKTIMTMADPTTPQNGFVDTKAPNGQQFYRLYIMLEGG
                     RYLFSTAKKPLNDTNYLRRLTIDTSMLRNMSRDSLLRLRAGLTNVNRPDLGKANIQDS
                     LFILDSLLRVASIPVVIKLGSFQTGDSASLPNAVTIKNRPTAFEPSLYVYTNKDGYVK
                     VSLPEEGNKKYMIRFLEENNSLIFELKDIKTKNFKIDKANFFHAGWFRFEIYEDGKLL
                     EKHKFYLEKDF"
     gene            complement(6628..7335)
                     /gene="lipB"
                     /locus_tag="PLU11_07605"
     CDS             complement(6628..7335)
                     /gene="lipB"
                     /locus_tag="PLU11_07605"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPN59020.1"
                     /translation="MEKQKINFRDLGQMNYQSAWDLQESLLKENVRRKSVPETGESTD
                     HYLLFVEHPPVYTLGKSGNKENVLLSDEELQANQIEFFHTNRGGDITFHGPDQIVGYP
                     ILDLEKFYTDIGKYLRNIEEVIILTMAEYGIKGERSSGETGVWIDPDLPGRERKICAI
                     GVRCSRWITMHGFAFNVNTDLSYFNHIIPCGIVNKQVTSLQRELGRELDMQEVKEKVK
                     RNFESVFEACLVSEVLL"
     gene            7399..>7767
                     /locus_tag="PLU11_07610"
     CDS             7399..>7767
                     /locus_tag="PLU11_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPN59021.1"
                     /translation="MDQLLPENNEGSDELYERFTLTIDKGQEPLRIDKFLSNRIEGAT
                     RNKLQRAMNLGMVQVNGVAVKPNYKIKPLDYVVIYSDMSPDETDVVPEKMDLNIVYED
                     TDLMVVNKPAGMVVHPGSGNY"
BASE COUNT         2208 a         1814 c         1695 g         2050 t
ORIGIN      
        1 tcattttctt ggccgccccg gcttcaattt ccacccagct gttcatctct ttcatatcga
       61 tacgaccaag tacatccttt ttcaaatcac tcatatcgag aatgaactgc aggaaactgg
      121 ctttatagaa accatctttg gttccaagat ttacgaacag tcgttgataa gaacctccgc
      181 cgccgccaaa ctttcttccc tgagtatcgc gttgctcaaa acgggctgct cctctttcgc
      241 cccgctctgt tttcttaccc gcatcccgca aattcaggtc agctgcattt tcataatatt
      301 taaggaaacg gtcaaactcc agtgcagcta cccgttgcag aatttcttct ttctccacat
      361 gggcaaattt ctctcttaat ataggcaggt aggtttcata ctctccatgg ctgatatccg
      421 cacccagcat tttatcaatg aaagagaaaa actgtttgcg gcatacatcc tttccgctcg
      481 gaatatccat tttatgcacc cgggtattca ccagtttttc gatctggcgt aaccggtaaa
      541 cttctttggg tgttacgata gatacagaca ccccgctgtt accggcccgg ccggtacggc
      601 cgctgcggtg agtatatact tccacatcat ccggtaattc aaaattgatt acgtgtgtaa
      661 ttccctgcac atcaatacca cgggcggcta catcggtggc ggccagcagt cgcacactgc
      721 ggtcgcggaa cagccccatt actttatccc gttgctgctg cgtcaggtca ccatgaatgg
      781 catcaatatc atatccttca cgggtcagtt tctcggcgat gtcctgcgta tcggccttgg
      841 tacgggcaaa cacaattcca taaatgccgg gattaaaatc aataatccgc ttcaatactt
      901 catagcggtt atgatgctgg gtcatataat agtggtgatc aatattggca ttcccctcgt
      961 tcatcttacc aatggtaatc tcgaagggtt tatccatata ccgtttgctt acctggcgaa
     1021 tttccgcagg catggtggcg ctgaataacc aggtgctttg acggttcggg gtattcttca
     1081 gaataaattc gatatcctct ttaaagccca tgttcagcat ttcatcggcc tcatccaata
     1141 ccacataatg gatctgttcg aggttgatcg ctttacgttc gatcagatca atcagccgac
     1201 cgggggttgc taccacgata tgggtgcccc ttttcaggtc acggatctgc atactgatgg
     1261 aggtaccgcc ataaacggca gtaacagaaa ctgaaacttt tttattcttg aatttttcca
     1321 ggtcactggt gatctggaga cagagttccc gggtgggaca tacaatcagt gcctggggga
     1381 aacggtcttc ttttttaatt aactggagga gtggtaatcc gaaagcagcc gttttaccgg
     1441 tgcccgtctg ggccagtccg ataaaatcct tggttccctg gagcagaacc ggaatggctt
     1501 tttcctggat cggggtgggc tgggtaaacc ctaaggtttc gatggaaagg agtaatcctt
     1561 cctcaatacc cagttcttga aatttgttca cttgataata atatttgtat aaataaagcg
     1621 caaaggtagg ctttcagggc gggaaagtcc gtacttacgg acttaagggc ttgcggtcat
     1681 tccgtttccg gacttgtaaa aaaagaaaag ccgtcccgta gtccgggacg gccttcagcg
     1741 atttgcttag agtaaattac tttgcagaat ctttcttagc agcagtgtca gctggaactt
     1801 tagcagcagt atccatagct ttagcagcag aatcagcagc tactttagcc agtgaatcag
     1861 cagtagctac tttagccaga gaatcagcag tagcatcttt agattcagta ctgttgttac
     1921 aagctactgc gaaaagagcg atagctgcga ttgccagaaa tttcttcatt ttcaataatt
     1981 ttttgttttg tttgaaatat ttcgcttttt ataccggaaa cgggaaaagg taacccggtt
     2041 tttaaaaaat atttttttgg gctgctcaag ggttactatt tttaaaaacc cgtattaaat
     2101 aagggaatgt gaaacaagcg attaacgaaa aggaattatt gcagggatta gccgtaaacg
     2161 acaaaaaagc cgttgaaacg atctacaagg agaactataa tatggttcaa tccttgataa
     2221 tcaataacaa cggttcggca gatgatgcca aagacatatt ccaggaggcc atgattgttt
     2281 tgtacgaaaa agtgcgctcc ggaagctttg aactcaactg cctgatcaag acctatgtat
     2341 actctgttag taaaagactg tggctcaaaa ggttacagca gttaaaccga tattcacctc
     2401 cgatcgacga cctcgaatcc ccggtacctg tggatgagga tatggaagac catgagcgaa
     2461 gagatgctga gtttgaaatg atgaacaaag ccatcagcag cctgggagaa ccctgcaaaa
     2521 gtttgttaga agcttattat ttgcaaaaac agaacatgca ggtcattgcc gccaattttg
     2581 ggtataccaa tgcggacaat gccaaaaacc agaagtataa gtgcctgatg cggttgaaaa
     2641 agatattttt tagtcattat aaaaacgaga acggaaatgg gtgatactaa agtacttgag
     2701 gcggtggaac gctatatccg tggggagatg aatcctgacg aacgtttgca ttttgaaaat
     2761 ctgaggaaat caaacggtga aattgatgaa ttggtggtag aacacacttt attcatgcaa
     2821 cagatgaatc gttttggtga atggaagaaa ttccgcagta ccctgcacga tgtacatact
     2881 gacctggccg aacaaggaaa gatcaattcg gcccgcctga ccggtaaagc caaagtggtt
     2941 tacctctgga atagatataa aagagtggca gcgatcgcag cctctatcgc cgggattact
     3001 actcttacga tcagtgccct tgtctggact attacccctc aaactgcacc tggtgaactg
     3061 caacagctga aagggaagat tactattatt gaaaatacca ccaaagcaca gggacaggaa
     3121 atcaataaag taaaaaataa aataagcagt tctgataaca ccattgtata taccacaggt
     3181 ggtaccggct ttatcattga taccaaagga tacctggtta cgaatgtgca cgtggtggaa
     3241 ggcgctaaaa atattgctgt acagaacagc cggggtgagt acactgcaaa agttgtttac
     3301 attgatgccg caaaagatat cgctattctg aagattgatg acgaatcatt caaaccttat
     3361 tcgtcaatcc cttacacgat cagccgctca tccggcaaac tggctgaacc catttttaca
     3421 ctgggctacc cccgcaacga agtggtgtat ggtcaaggct acctgagttc aaaaactgga
     3481 tacaaaggcg acacgctcag ctgtcagctt gaactgaccg ccaatcaggg taacagcggc
     3541 agcccgatcc tgaataaaaa aggagaagta atcggtatcc tgaatggccg ccagaaaaat
     3601 gcagaaggat tctcattcgc tattcagggc aaacatattt acaaagtgct tgaagacctg
     3661 aaaaaagatc agaaaaagga taccagttat cagcggatca aactgaactc ccgctcctca
     3721 atttctggaa tggatttcca gcagcaggtg gaaaaagtgg aagactatgt attcatggtt
     3781 aaagtcaatt aataacagat tacccctata gaaaaaaaga agctgtatca ccggatacag
     3841 cttctttcgt ttataagcac tttactgatt accacaattt cgccgggtac tccccttttt
     3901 caatcagcgt attcaggctg ttctgtacaa agggggcatc ctccttatat gttaccccaa
     3961 accagttgga tgtagtcggg ataatctcca ctttgccgcc ttccttaata aactgatccg
     4021 ccacaatggg aataaagaat tcagacttca gttcctgacc ggaggcgtgc aggaattcaa
     4081 taaataattt ctgcgtatag gcaaagaagg aaggatcaaa acaccagaaa ttcatggaaa
     4141 ccctggtttc aagagataat tctttcggtt ccaaaccgtc ctcacataaa atcttaccct
     4201 cttttttggc aatattgatc cgctcagtca ccgtggccag gttcccgtcc gcatccaatc
     4261 cgcaaactcc gcggttcacg gtaccatggt cagataaggt acgcaacagg tcatagccca
     4321 caatcgcata tacattctcg ttacaaccat tattcagaaa cgacgccgca ttttcaaagg
     4381 cattgctgcc ataaaagtca tccgcattga tgacggcaaa aggctctttc accacttctt
     4441 tggcacaaag caccgcatgg gccgtacccc agggtttggt ccgctcagcc gcggccttat
     4501 acccttccgt ataataatcc atttcctgaa atacatactc agtctgtact ttcccgttta
     4561 atttcggctc aaatatggtt ttaaaattat cggcaaattc tttccggatg ataaagacca
     4621 ctttgccaaa gccggcacgg atcgcatcat atatagaata gtccataatg gtttcgccat
     4681 tggggccaaa gccctggacc tgtttcatac tcccgtaacg ggaagccata ccggcagcaa
     4741 ggattactaa tgtaggtttc ataatgagga tggttttgcc ctgcgaaatt aatctaattt
     4801 agcaaagagt ttactaaaca tgcagtatat aaatccggaa catataaggg tagaccccta
     4861 tttatcacca ctctttgatg ataaagagat taccgcgaat atcctgcggc ttgatttgct
     4921 gcaccccgtc gtgtccggaa ataaatggtt caaactccgg tattatattg aaaaggcaca
     4981 ggaacaaaat aaaaactcgc tcgtcacttt cggcggtgcc tggtccaatc atattcatgc
     5041 caccgccgct gccggaaaaa tggttggatt aaaaaccatt ggtatcatcc ggggtgaagc
     5101 gcccgcccga ttatccacca ccctcctgca ggcaacggag ctggggatgc agttacactt
     5161 tatcagccgg gaagaatacc ggcaaaagcc aatcccggaa agtattgatt tatcctcatc
     5221 ccagttgatc cctgaaggcg gatacggcac tactggggcc aggggtgctg ccacgatcct
     5281 tagtttgata aacccggatg actacacgca tatcgtttgc gccgttggct ccgggacaat
     5341 gatggctggg cttatgaatg catactggtc aaaaggagta gtgctcggaa taagcgccat
     5401 gaaaaacaat caggcactgg aaagcgatgt tcgtcaactg ttggaggttc ccgctatttc
     5461 tccggtaatt attcatgatt atcattttgg cggctttgcg aaatataaca gccggctgct
     5521 tgattttatg aatgaagtgt atcggtatac cggcatccct accgatatcg tctataccgg
     5581 aaaactattt tttgccctaa acgacctggc ctggaaaaac tactttcccc cgggcagtaa
     5641 aatcctgcta atccatagcg gaggtttaca gggaaaccaa tctttaccga atggatcgtt
     5701 aatattttag aatttctgaa ggaggaacct atctttgttt ttcaccggaa aagaaaccgg
     5761 aaactattcc gtttatacaa tatgaaaaaa gggtccctgt attttttcct ccttcttgcg
     5821 gtttgttccg tcagtaacct gtttgcgcag gatacccttc ctaaattttc ccttaaaaac
     5881 gttggaaaaa acaggatcgt ggtcagctgg accaatccct tccccactat cggccaactg
     5941 agtatccagc gatcgtacga cagtatcaaa ggatacaaga ccatcatgac catggccgac
     6001 ccgaccacgc ctcagaatgg ttttgtagat acaaaagcac ccaacggaca acaattttac
     6061 cggctctata tcatgctgga aggtggccgc tacctgttca gcacggctaa aaaaccgctg
     6121 aacgacacca attatcttcg gcgactgacc attgatacgt ctatgctgcg gaacatgtcc
     6181 cgggatagtc tactgcggct gagagccgga ttgaccaatg taaaccggcc ggatctggga
     6241 aaagccaata tccaggattc gctgtttata ctggactccc tgttaagagt tgcctcgata
     6301 cccgtagtaa tcaaattagg cagctttcag accggcgact ccgcctccct gcccaatgca
     6361 gtgaccatta aaaacaggcc gaccgctttt gaaccctctc tttatgttta caccaacaaa
     6421 gacggatatg tgaaagtgag cttgccggaa gaaggaaata aaaagtatat gattcgtttt
     6481 cttgaagaga acaatagcct gatctttgaa ctgaaagaca tcaagaccaa gaactttaaa
     6541 attgataaag ccaatttttt ccatgccggt tggttccgtt tcgaaatata tgaggacggc
     6601 aaactactgg agaaacataa attttatcta gagaaggact tctgatacca ggcaggcttc
     6661 aaatacactt tcaaaatttc gttttacttt ttcttttact tcctgcatat ccagctcccg
     6721 acccagctca cgctgcaggg aagtcacctg tttgttgaca atcccgcagg ggatgatatg
     6781 attgaaatag gaaagatcgg tattcacatt aaatgcaaat ccatgcatgg tgatccaacg
     6841 gctgcagcgc accccaatgg cacagatttt tctttccctg cctggcagat ccgggtcgat
     6901 ccatacaccc gtctctccgg atgaacgctc cccttttatg ccatattctg ccatggtcag
     6961 gataatcact tcctctatat tccggaggta tttaccgata tccgtataaa atttttccag
     7021 atcaagaatc gggtacccca cgatctggtc ggggccgtga aaggtaatat cccctccccg
     7081 gttggtatga aaaaactcaa tctgattggc ctggagttcc tcatcactga gcaaaacatt
     7141 ttctttattc ccgctcttcc caagggtata aacaggggga tgctccacaa aaagcaggta
     7201 gtgatcagtg gattcacctg tctccggaac tgatttacgc cgcacatttt cctttaaaag
     7261 ggactcctgg aggtcccagg cggactggta attcatctgt ccaaggtccc ggaagttgat
     7321 tttttgcttt tccatcgtta aaaagcaaat ttaccaacaa atttccatcc tttacctttg
     7381 ccggctatgc aggaagaaat ggatcagctc ctaccggaga ataatgaagg cagtgatgaa
     7441 ctgtacgaac gctttaccct taccattgac aagggacagg agccgttgcg gattgacaaa
     7501 tttttgtcca accggatcga gggggctacc cgcaataagc tgcaacgggc aatgaacctg
     7561 ggcatggtgc aggtaaacgg agtcgcggtc aaaccgaatt ataagattaa gccactggat
     7621 tatgtcgtca tctactccga tatgagtccg gatgaaaccg atgtggtgcc tgaaaaaatg
     7681 gacctgaata tcgtgtatga agataccgac ctgatggtgg tcaataaacc tgccggcatg
     7741 gtggtacatc cgggcagtgg caactat
//
LOCUS       DAOPMW010000129         7757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10556_length_7757_cov_5.940535, whole genome shotgun sequence.
ACCESSION   DAOPMW010000129 DAOPMW010000000
VERSION     DAOPMW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10556_length_7757_cov_5.940535"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1166
                     /locus_tag="PLU11_07615"
     CDS             51..1166
                     /locus_tag="PLU11_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59022.1"
                     /translation="MRFIDNQNDRETKEGDKPSRKKPMYPVNTKLRSYLRERGREVRL
                     PVTYHDLLRFTWSTPIMDKNGNHTHWEKASYDSRDWDYIREGLVKIYAILKTEGDFSF
                     ITHLDVARIDYCSFGNSNPFRIRIVNKFNDNYDHYYIKIADASRIYGLELEHILSPNR
                     ITFFTYGNTLVEEHIPGIPGDMFIRQILESPETNKIRLAKEFVKFNERCFVRLLGDMR
                     SYNFVVSITPDIEDYQYRIRSIDFDQQSYEGRKNLYLPQFFKENYAFVESALAVLNRE
                     SIEQYQAEERTMITFRLASSRYRIKDLLDIMTHDKISTPEKVAQLKKELSEYMKTTDY
                     DKCQNMGQVVKVHLKLTLRKNLLLVQKNLGKSKRKSR"
     gene            1278..2345
                     /gene="fbaA"
                     /locus_tag="PLU11_07620"
     CDS             1278..2345
                     /gene="fbaA"
                     /locus_tag="PLU11_07620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPN59023.1"
                     /translation="MKKYRAGVLFGDELEALYNDARDNQFALPAVNTIGTNTINAVLE
                     TAAKVNSPVIIQFSNGGAQFIAGKGMPNDNLQGNIAGGISGALHIHNVAKYYGVPVVL
                     HTDHAAKKWLPWISGLLDAGELYHKEKGQPLFSSHMLDLSEEPIEENIHTSVEFFKRM
                     NPLGMSIEIELGVTGGEEDGVDNSDVENSKLYTQPEDVAYSYRELSKVGKLFTVAAAF
                     GNVHGVYKPGNVELRPVILNNSQEYIQKEFKTAAKPVYFVFHGGSGSPQHQIREAIGY
                     GAIKMNLDTDLQWAFWEGVLDNYKKHEAYLQGQLGNPEGDDKPNKKYYDPRVWLRKGE
                     EAFIKRLEVAFEDLNCINRNA"
     gene            2580..3806
                     /locus_tag="PLU11_07625"
     CDS             2580..3806
                     /locus_tag="PLU11_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59024.1"
                     /translation="MKTKFYPFITLFAFICCAGLVQGQTVCNNETIRFRETFGQTPLT
                     EGYEEGKTGFKFMSGAELETGEYAVLGNTQVRPEWHNSTDHTGDLNGQMMIINPGYAE
                     ADFFADSIENLTTAGYYSVSLYVMNVTKAGTCGAGAVLPRLQIQIEYLSGLTAYQHLS
                     GFTSDFIPSSTNPGWIKVISGFVLPPGIQNIRYRIINQSMGDCGNSLAIDDITFSQCA
                     SLSTLPVKGLKINSIEPAGSGTRILFSTESESQTDRMITQKSTDGINWTDIHSQAAAG
                     NNDQYRAYTASDIYVASPVIYYRIQQTDLKGGVSYSAIVKYTAGSLSSTTLTAYPTPF
                     TSQLHLNFTSLKNETYTATLYAANGLALQSSTLNARKGSNLIQFNTINLKQGTYLVTV
                     VNSDGSIRLTQKTVKQ"
     gene            4030..5253
                     /locus_tag="PLU11_07630"
     CDS             4030..5253
                     /locus_tag="PLU11_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59025.1"
                     /translation="MKTKLYFSSLVTTLLLTFSSNAQTVCNGEAIRFRETFGTGNSAA
                     SLPAGRTTYTYNGSSDLADGYYRLSKSSRGRSEWHNAADHTGDANGRMMVINAGFTAG
                     EFYKDTITNLTPGASYSVSLYVMNVNTLGTCGSGAILPKLQFVVEYLAADNKFQSLTT
                     FTTDFIPQSSNPSWVKITSGFMMPGGITSVRYRIINNSNGGCGNDLALDDITFSQCAS
                     LSSLPVKGLKINTVEESGTGTRLLFSTESEYNTSMMETQKSTDGITWVTIHQQPAAGI
                     SDRYKAYSSNDNEATTNDVYYRIRQIDIDGTFTYSAVVSFKGGGKSGNTMTAYPNPFS
                     AKLSVNIVSAENDNYLITLYNAAGAQIQQIKLTARKGSNLAEFNGTQLRNGMYLVTAV
                     NSDGRIKFSQKLIRN"
     gene            5486..6265
                     /locus_tag="PLU11_07635"
     CDS             5486..6265
                     /locus_tag="PLU11_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN59026.1"
                     /translation="MGKVLIITGGNKGIGQGICEAYKNNGYQVFSISRSQLLADSSQN
                     VNYIQFDLQQTKDVTAVLEEILMGLPKREMDSITLINNAATTGPIRRFEETSSADIEK
                     VISLNVVAPLMLTSGFIQYTKGWNCRRHIINISSGAAINPLAGISTYCVSKAAIDMLT
                     KSIALEQAGNSGNFKIISVYPGLVDTDMQRDIRTSNSTHFPEVKTFREFKRNGDLAGK
                     YDVGNEIYHIDHNVLVTNGSILDLDVYRYSTQKLHAVPSIT"
     gene            6378..6815
                     /locus_tag="PLU11_07640"
     CDS             6378..6815
                     /locus_tag="PLU11_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59027.1"
                     /translation="MIRIKFIPVLIGIALLTACGSKDEKATPDVPAAGTTPPPAQLQP
                     LPAQGTTNAPNPASIALNPEHGQPGHRCDIAVGAPLNSPAAQPAPVPAPVKQTMPQQP
                     VQTPAPSLNTTTPSKGVLNGAVNPAHGQPGHRCDIAVGAPLNS"
     gene            6835..7344
                     /locus_tag="PLU11_07645"
     CDS             6835..7344
                     /locus_tag="PLU11_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59028.1"
                     /translation="MPRPDLNRVPVFYHKYIAQVEGDNINEILSKQAITILALLNNLP
                     AEKRDYRYGPDKWTVKEVLQHIMDAERVFAYRALCIARGEQQALPGFDENLYADKAQC
                     TNRKWSDMTEEFRAIRRSTEILFASFNDSQLDATGTASGWPVYVSGIGYLTAGHAQHH
                     LNILLERYL"
     gene            complement(7417..>7757)
                     /locus_tag="PLU11_07650"
     CDS             complement(7417..>7757)
                     /locus_tag="PLU11_07650"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HPN59029.1"
                     /translation="LIAETTDWLSKQSEKKYSLQLEKYRKEQEKVKASVKQLETLLKL
                     KGQLDVSALPAETNRWAEDKSKQERFQLWLKSLQKDIYLDQTMKVMNDIISQQNLVKG
                     KTEEPVKKAF"
BASE COUNT         2238 a         1834 c         1643 g         2042 t
ORIGIN      
        1 actaagacag gactatttaa ataaaatatc ttttagaaca gtttactaaa atgcgtttca
       61 tcgataacca gaatgaccgg gagaccaagg aaggtgataa accttcccgg aagaaaccta
      121 tgtacccggt aaataccaag ctcagaagct atctgcgtga acggggaagg gaagtcaggc
      181 tgcccgtaac atatcacgat ctgctccggt ttacctggtc cacccctatc atggataaaa
      241 acgggaatca cacgcattgg gaaaaagcgt cttatgacag ccgggactgg gattatatcc
      301 gggaaggcct ggtgaagatt tacgcgatcc tgaaaacaga gggggatttc agttttatca
      361 ctcacctgga tgtggcgaga atcgattact gctcttttgg gaattcaaat cctttccgga
      421 tccggattgt caataaattc aatgacaatt acgatcacta ctatatcaag attgccgatg
      481 cctcgcggat atacggcctg gaactggaac atatcctgtc acccaaccgt attacttttt
      541 tcacgtatgg taatacattg gtggaagaac atatacccgg tatacccggt gatatgttta
      601 tccggcagat cctggaatca ccggaaacca ataaaatcag gctggccaag gaatttgtaa
      661 aatttaatga gcgttgtttt gtccgcctcc tcggggatat gcgcagttat aattttgtgg
      721 taagcatcac cccggatatt gaagattacc agtaccggat ccggagtatt gattttgacc
      781 agcaatccta tgaaggccgg aaaaatctgt atctgcccca gttttttaaa gaaaactacg
      841 cctttgtaga atccgctttg gccgtactga accgggaatc cattgaacaa taccaggcag
      901 aggaacgaac catgattaca ttccggctgg ccagttccag gtaccggatc aaggacctgc
      961 tcgatatcat gacccatgat aaaatttcga cgccggagaa agtggcccag ttgaaaaaag
     1021 agctgagtga atacatgaaa accacggatt atgataagtg tcagaatatg ggtcaggtgg
     1081 tgaaagtaca cctgaagctc accctccgca aaaatctgtt gctggtgcaa aaaaacctgg
     1141 gaaaaagcaa acgaaaatca cgttgaagtc taacagatgt tagctttgta aataacggaa
     1201 aagctctata ttagcgggcc tttaacgatt acctgcctgc tgaaaagcat ttgccacttt
     1261 aaaaatttaa aaaatcaatg aaaaagtaca gagccggggt gctcttcggc gatgagctgg
     1321 aagcactcta caatgatgca agggataacc agtttgcctt acctgctgta aacacgatag
     1381 gcaccaatac cattaatgcc gtactggaaa ctgcagccaa ggtgaattcg ccggtgatca
     1441 tccagttttc aaatggcggg gcccagttta tcgctggtaa aggaatgccc aatgataacc
     1501 tccagggaaa tattgccgga ggtatttccg gtgccctgca tattcacaat gtggctaaat
     1561 actatggagt acctgtagtc ctgcacacag accatgcagc caagaaatgg ttgccctgga
     1621 tcagcggttt gctggatgca ggagaactgt accataaaga aaaaggacag cccctgttca
     1681 gttcgcatat gctggacctg agtgaagagc caattgaaga aaatatccac acttctgtgg
     1741 aatttttcaa aaggatgaat cccctgggta tgagtattga aattgaactc ggagttaccg
     1801 gcggtgagga agatggcgtg gataacagtg atgtggaaaa ttccaagtta tatacccaac
     1861 cggaagatgt ggcttactct tacagggaac tcagtaaagt aggaaaactc ttcaccgttg
     1921 ccgctgcctt tggtaatgtt cacggagttt ataaacccgg caatgtggaa ttacgcccgg
     1981 tcatcctgaa taacagccag gaatacatcc agaaagaatt caagaccgcc gccaaacccg
     2041 tatactttgt tttccacggc ggaagtggct caccccaaca ccagatacgt gaagcgatcg
     2101 gatatggcgc catcaaaatg aatctggata ccgatctgca atgggcattc tgggaaggtg
     2161 tactggataa ctataaaaaa cacgaagcct acctccaggg ccagcttgga aaccctgaag
     2221 gagacgacaa accgaataag aaatactacg acccgcgcgt atggttgcgt aaaggagaag
     2281 aagcctttat caaacgcctt gaagtggcct ttgaagacct caactgtatt aacagaaacg
     2341 cataaacgaa aagcccggcc aaaagccggg ctttttctta cccctcctgc ctactgccta
     2401 ctgcctcctg cctactgctt actgcctcct gcctactgcc tcctgccccc cgttccattc
     2461 tgtataaaat cattccataa tctggaaatt ttcatcccat aacaatttct atattattga
     2521 atttcaggtg attaaaaatt ggcacactag tggtgattcc tcctatgaaa atcgtactta
     2581 tgaaaacaaa attttaccct tttatcacat tattcgcgtt catttgctgc gctggattag
     2641 tccagggcca gacggtctgc aacaatgaaa ccatccgctt ccgggaaaca tttggccaaa
     2701 ctccgctgac tgaaggttat gaggagggaa aaaccggttt taagtttatg tctggggctg
     2761 aactggaaac cggtgaatat gctgttcttg gcaatacaca ggtccgtccc gagtggcata
     2821 acagcactga tcataccggt gatctcaatg gccagatgat gatcattaat cccggttatg
     2881 cggaggctga tttttttgca gacagtatcg agaatctgac tacggccggt tattattccg
     2941 tttccttgta tgtgatgaat gtaacaaaag ccggtacctg tggagccgga gctgtgcttc
     3001 cccgcttaca aatacagata gaatacctgt ccgggctaac cgcttaccag catttatccg
     3061 gttttacttc cgatttcatt ccctcgtcaa ccaatccggg atggatcaaa gttatcagcg
     3121 gttttgtact gcctcccggc atccagaaca tccgttaccg tattatcaac cagtccatgg
     3181 gagattgcgg caatagcctg gctattgatg atataacctt ttcacaatgt gcaagtcttt
     3241 ccaccctgcc ggtaaaaggg ctgaaaataa acagtattga gccagccggc tctggtaccc
     3301 ggattctctt ttccaccgaa tcagaatctc agactgaccg gatgatcacc caaaaaagta
     3361 ccgatggcat taactggacg gatattcatt cacaggctgc agccggtaat aatgaccagt
     3421 accgcgctta tacagcctcc gatatctatg ttgcttctcc tgttatttac taccggatac
     3481 aacaaacgga tttaaaaggt ggagtgagtt attccgccat tgttaaatac accgccggca
     3541 gcctttcctc caccacgctg accgcttacc ctactccttt tacaagccag ctccacctca
     3601 atttcaccag tctcaaaaac gaaacctata ccgccaccct gtatgcggcc aacggacttg
     3661 ctcttcaaag cagtacctta aatgcacgaa aaggaagtaa cctgattcag tttaatacga
     3721 tcaacctgaa acaaggcacc tacctggtaa ccgtggtgaa tagtgatggc agcatccggt
     3781 taactcagaa gacggttaaa caatagtctc tcttaataat tccatattgc gattttcacc
     3841 gcgggtaaat cccgcggttt ttttatatct gcactataga gagttttctc atatatactg
     3901 gaatacttcc atatttgaga atcagtctaa ttattggact atataacatt attttgttat
     3961 tcgtatattg ttgtttttat tatttttatc gattggcaca gcaatggaat actagggaaa
     4021 aaacgtacaa tgaaaacgaa actctacttt tccagcctcg taactaccct tttactgaca
     4081 ttcagttcca atgcccaaac cgtttgtaat ggtgaagcaa tccgcttcag ggaaacattt
     4141 ggaaccggaa atagtgctgc ttctcttcct gccggcagaa ccacatatac ctataatgga
     4201 agttcggatc tggctgatgg ttattatcgc ctttcaaaaa gctcaagggg acgttctgaa
     4261 tggcataatg cggcagacca taccggtgat gccaatggca gaatgatggt aattaatgcc
     4321 ggttttactg caggagaatt ctataaggac accatcacca accttactcc tggtgccagc
     4381 tattccgttt ccctttacgt aatgaatgta aatacgttgg gaacctgtgg aagtggcgct
     4441 atactcccta aactgcaatt cgttgtggaa tacctcgccg ccgacaacaa atttcaatca
     4501 ctgaccacct ttaccactga ttttattccc caaagcagta atccttcctg ggtaaaaatt
     4561 accagtggct ttatgatgcc cggtggtatc acgagtgtcc gttaccggat catcaataac
     4621 tcgaatggcg gatgcggaaa tgatctcgcg ctcgatgaca ttactttttc ccagtgtgcc
     4681 agcctttctt ctttaccggt taaaggatta aaaattaaca ctgtggaaga atccggaaca
     4741 ggcacccgtc tccttttctc caccgaatca gaatataata catccatgat ggaaacccag
     4801 aaaagtacgg atggcattac ctgggttacc attcatcagc aaccagctgc tggtatcagt
     4861 gaccgctaca aagcctatag cagcaacgat aatgaagcta ccaccaacga cgtatactac
     4921 agaatccgcc agattgatat tgatggaaca tttacttact ctgctgtagt gagttttaaa
     4981 gggggtggga aatccggaaa taccatgacc gcttacccaa accctttttc agcgaaacta
     5041 tccgtgaata tcgtttcggc agaaaacgat aactacctca tcactttata taatgcggca
     5101 ggtgcacaga ttcagcaaat aaaactgact gcccgtaaag gaagcaacct cgccgaattt
     5161 aatggcaccc aattacgtaa tggcatgtac ctggttaccg ctgtcaacag tgatggccgc
     5221 attaaattca gccagaagct gatcagaaac taaatcattg tttattcgtt tttttagccg
     5281 cgggttctcc tgtggttttt ttttcctgac aggtctgtca cgcttaagaa gtgcatttgc
     5341 tataatctgt gaatattata ataaccatcc cggattgtgt aatgggtgaa aaaaatggac
     5401 agattatttt taattgaaat taatcgggta tacaatcact attcaacaat tgtctcgaag
     5461 ccgtttttca gagttaaata atagtatggg taaagtgctg atcataaccg gaggaaataa
     5521 aggaatcgga caaggcatat gcgaagctta caaaaataat ggctaccagg tcttctccat
     5581 ttccagaagc cagcttttgg ctgacagttc acagaatgta aactatattc agtttgatct
     5641 tcagcagacc aaagatgtta ctgctgtatt agaagaaatt ctcatgggac tgccaaaacg
     5701 ggagatggac agtatcaccc tgattaataa tgcagccact accggcccga tcaggcgttt
     5761 tgaagaaacc agttccgccg atatagagaa agtaatcagt ctgaacgtgg ttgctccttt
     5821 aatgctgact tccggtttta tacaatatac aaaaggatgg aattgccgcc ggcacatcat
     5881 taacatttct tccggcgctg ccataaatcc gctggctggc ataagtacct attgtgtgtc
     5941 caaagcagca atcgatatgc tgacaaaatc cattgcactt gaacaagccg gcaactccgg
     6001 gaattttaaa attatatccg tttaccccgg ccttgtggat acggatatgc aacgggatat
     6061 ccgcacctca aacagcaccc attttccaga agtaaaaaca ttccgggaat ttaaacggaa
     6121 tggtgacctg gccggaaaat atgatgtagg gaatgaaata tatcatattg accataatgt
     6181 cctggtaact aacggatcca tactcgactt agatgtatac cgctactcca cgcaaaaatt
     6241 acatgctgtt ccttcaatca cctgagccac aggtatcgaa attccataag cgctgatttt
     6301 tgtcactgct gaagcagttc tggcaaatct tccttatttt acaggccgca ttattattca
     6361 attttaaaaa aataaggatg atccgaatta aatttatacc ggtattgata ggcattgccc
     6421 ttttaaccgc atgtggaagt aaagatgaaa aagccactcc ggatgttccg gctgcaggta
     6481 ccacgccacc acctgcccag ttacaaccac tcccggccca gggtacaacc aatgccccta
     6541 acccggccag tatcgcttta aaccctgagc atggacagcc cggccatcgt tgtgatattg
     6601 cggttggtgc cccgctgaac agtcctgcgg ctcagcctgc tcctgttccc gctccagtca
     6661 agcagacgat gccccaacaa cccgtacaaa ctccggcccc ttctttaaac acaaccaccc
     6721 cttcaaaagg tgttttgaac ggtgctgtga atccggccca tgggcaaccc ggccaccgtt
     6781 gtgatattgc ggttggcgcc cctttgaata gttaacttgt aaattaaaat tgctatgcca
     6841 cgtccagatc tgaaccgggt tccggttttt tatcataagt acatcgccca ggtggaaggt
     6901 gataatatca acgaaatctt aagtaaacag gcaataacca ttttagccct actgaataat
     6961 ctgcctgccg aaaaaaggga ttaccgctat ggaccagaca agtggactgt taaggaagta
     7021 ttacaacata tcatggatgc agaacgggta tttgcctacc gggctctttg tattgcccgc
     7081 ggcgaacaac aggcattacc agggtttgat gaaaacctgt atgcggataa agcacaatgc
     7141 accaaccgaa aatggagtga tatgaccgaa gaattccggg cgatccgccg ctcaacggaa
     7201 atcctgtttg cttcgtttaa cgatagccag ctggacgcaa caggtactgc cagcggctgg
     7261 ccggtgtatg tatcaggtat cggctacctg actgccggcc atgcacagca ccacctgaat
     7321 atattactgg aacgatattt ataaaacgca ggttttacca aataaaagcg ggtgtaccag
     7381 aagcttctga tacacccgct ttattttata tactccttaa aaagccttct ttaccggctc
     7441 ttctgttttg ccttttacca ggttctgctg actgataata tcattcatca ctttcatcgt
     7501 ctggtccagg tagatatcct tctgcagact tttcagccac aactgaaaac gctcctgttt
     7561 tgacttatcc tccgcccaac gattcgtttc tgcaggcagg gcgctaacat ccaactggcc
     7621 tttcagcttc aataaagttt ccagttgctt cacactggct tttaccttct cctgctcttt
     7681 gcggtatttt tccagctgga gcgaatactt cttttcactt tgtttggata accaatcagt
     7741 ggtctctgca atgaggc
//
LOCUS       DAOPMW010000130         7720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10646_length_7720_cov_8.685453, whole genome shotgun sequence.
ACCESSION   DAOPMW010000130 DAOPMW010000000
VERSION     DAOPMW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10646_length_7720_cov_8.685453"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1462)
                     /locus_tag="PLU11_07655"
     CDS             complement(194..1462)
                     /locus_tag="PLU11_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN59030.1"
                     /translation="MNKIYIIIRREYLTRVRKKTFIISTLLFPLLYLALIFGMGFIAE
                     KSKQSLHVALIDSSGYFTAPLLSKENLADNNSMITLVKGNADSVIADHKKAGFDGYIV
                     VPAIDWKQGAALQFKANKAYGRSSTAAVEAKLNSIWTAIKKDSLQIDSLKQDILEQRL
                     MVTPKNIQDENANAQYAEAIGYVCGFLIYLILLIYGSQVMMGVMEEKTNRIAEVIVSS
                     VKPFQMMLGKILGIGLVALTQFLLWIAFVLIIYNVGKASGNAGMASEMVGKMQGLFTS
                     VNVPLILFCFGFYFLAGFFFYASLYGAIGSAVNEDMREAQSLSFPVTMLVIISIAMMS
                     AAIADPTGPIAVWGSIIPFSSPIVMMARIPFGVPGTVPWWQLILSMSVLILSFTFTVW
                     FAGKIYRTGILMYGKKPTWKEMMKWAFRKN"
     gene            complement(1486..2421)
                     /locus_tag="PLU11_07660"
     CDS             complement(1486..2421)
                     /locus_tag="PLU11_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59031.1"
                     /translation="MAILELQNLKKYFATQKAVDDISLNVERGQIFGLLGPNGAGKTT
                     LIRMITGIFYPDEGQIIFDGKKFDPVNDVGLIGYMPEERGLYKKMKIGEQAMYLAQLK
                     GLSHSDAQSKIKKWFARFEMDSWWNKKVEDLSKGMGQKLQFVTTVLHEPKLIILDEPF
                     SGLDPVNSNLIKDEIFNLAQGGSTIIFSTHRMEQVEEICNHIVLVNKGKKILDGTVKA
                     VKQEFKENIFSVGLNQPLTSVPATAPFELTGHMEDRHVIRIKDGSSPNDVLRYLLDQG
                     GNITAFNEILPSLNDIFIKLVEGTPTARQFQRVQA"
     gene            complement(2644..3129)
                     /locus_tag="PLU11_07665"
     CDS             complement(2644..3129)
                     /locus_tag="PLU11_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59032.1"
                     /translation="MQGFNKYGPLAAMYFQQSVEQTAFLTGGGKMVAPAQRLADFTEN
                     KISGSLPDCSYLPGIHSVSMKEVLPAFVSERLRLGFREFGKKMKGYITNEAVVVATES
                     RTSSPVRIPRNADNLQHPQIENLYPCGEGAGYAGGIVSAAMDGERVAAMILAKYSPQS
                     Q"
     gene            complement(3116..4213)
                     /locus_tag="PLU11_07670"
     CDS             complement(3116..4213)
                     /locus_tag="PLU11_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN59033.1"
                     /translation="MQKTFTLRLLPSEAASETLVKQSVALAAGVKPAAISGYILQKQS
                     IDSRGRQPWVNLTLLAYINEPCRERERKSFSFRDVHKATQTVIIVGAGPAGLFAALQL
                     IESGIRPLLLERGKDVRARRRDLAMLNKEGVVDPDSNYCFGEGGAGTYSDGKLYTRSS
                     KRGDINRILELFVHFGADEKILYESHPHIGTNKLPHIITAMREKILACGGEFLFEKRV
                     TDFILEGEKIKAVKTADGQRFEGDAVILATGHSARDIFELLHHKKILIELKPFALGVR
                     VEHPQSLVDSAQYHCDLRGEFLPPASYSWVQQVDGKGVFSFCMCPGGIIAPAATAPGE
                     LVVNGWSPSKRNNPYANSGIVVSIEEKGAGL"
     gene            4333..5082
                     /locus_tag="PLU11_07675"
     CDS             4333..5082
                     /locus_tag="PLU11_07675"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HPN59034.1"
                     /translation="MNPRYYFFPALLLFLTGISCHTSYEGQSLQYKGYRISQTMPADT
                     AVLHFLQPFSDSVNNSMNSVLGVTSESMEKAQPESSLGNFMADAFLIMAREKYQTQVD
                     LAFVNFGGIRLTQLPAGNITRGKVFELMPFDNLLILQKIKGSVLQQVLDLMAGRGGWP
                     VAGIRMQISNNKAVNVQVGGQPLDPEKVYTIANSDFIANGGDNADMLRPVPQITNGYL
                     MRDAIIDYIAWLNSNGKKITATIENRVTHAQ"
     gene            5072..6010
                     /locus_tag="PLU11_07680"
     CDS             5072..6010
                     /locus_tag="PLU11_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HPN59035.1"
                     /translation="MLSRKRFFKQAALTAGGLFAGPALLEAAEYFNPLRLTILHTNDT
                     HSRLEPFPMDGGRNQGMGGIAARARLISQVRQEEEQVLLLDAGDIFQGTPYFNIYKGE
                     PEIKAMTLLGYDACTIGNHDFDAGMENLAAQLTKHARFPMLVSNYDFSGTPMENKTEP
                     YRVIRKGKLTVGLLGVGIEGKGLIPDNLFGNTQYLDPVQKANETAELLRKKKNCDMVI
                     CLSHLGNQYKGSDKVSDEVLAKESENIDLIIGGHTHTFLDQPLVYKNKKGDEVVVNQV
                     GFAGLILGRLDFEFTKFSGKKLAKSHTISVSQKSGG"
     gene            6144..7577
                     /gene="dnaA"
                     /locus_tag="PLU11_07685"
     CDS             6144..7577
                     /gene="dnaA"
                     /locus_tag="PLU11_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPN59036.1"
                     /translation="MEKNADKIWSNCLKIIKDIVEWQHFKTWFEPIKPVSIKNNVLVI
                     QVPSQFFFEYLEEHYVNLLAKTLKRELGKEARLEYRIMVDSGNSKNKPVTMDVSGQGF
                     KTFSNNEMDFPLVINNPVKNPFVIPGLKKMQIDPQLNPNYTFEAFIEGDCNRVARRAG
                     KTVAEKPGANSFNPLVIYGGVGLGKTHLAQAIGNEVKRLHPNKVVLYVSSEKFINQFM
                     DHSRNNAINDFIHFYQLIDVLIIDDVQFFAKAEKSQDAFFAIFNHLHQSQKQLVLTSD
                     KSPKDLDGVQERLLSRFRWGLSADLQVPEYDTRIEILERKMKNDGLEMPKEVVKYIAY
                     NISNNVRELEGALISLLAQSSLNRREIDLDLAKKVLRNFVKSSSKEITIDTIQKMVCE
                     YFDVPYDKLLQKTRKREIVQARQITMYLAKAFTKNSLKTIGEHFGGRDHTTVIHSCQT
                     VKDLMDTDGVFRENVLELQQKVQLAAM"
BASE COUNT         2031 a         1783 c         1858 g         2048 t
ORIGIN      
        1 aagaactaaa tttttcatgt ttaggacggc agtgatgagt aatcagtaat gagtatgggt
       61 ggaagactcc actaataaaa acgccctccg attttcggag ggcgctatat attaagacta
      121 tacgatcagc tactcattac tgattactca ttactcattc ctctctcagc ctctttctgc
      181 ctttaaagcc ccctcaattc ttcctgaacg cccacttcat catctccttc cacgttggtt
      241 ttttgccata catgaggata ccggtgcggt agatcttgcc ggcaaaccag acggtgaatg
      301 taaaactgag gatcaataca ctcatggata aaataagctg ccaccagggc acagtgccgg
      361 gtactccaaa tggaatccgg gccatcatca cgatcgggga gctgaagggt ataatactgc
      421 cccatacagc aataggtccg gtggggtctg caattgccgc ggacatcatg gcaatggaga
      481 taatcaccag catggtgacc ggaaaactga gggattgtgc ctcccgcata tcttcattca
      541 ctgcactgcc gatagcgcca tacagggaag cgtagaaaaa gaaaccggcc aggaaataga
      601 agccaaagca aaacaagatt agcggaacat tcacactggt gaataaacct tgcatttttc
      661 ctaccatttc agaagccata ccggcattac cggaagcctt tcccacatta tatataatca
      721 gcacaaaggc aatccatagc aggaactggg tcagggccac caggccgata cccaggattt
      781 tacccagcat catctggaaa ggttttaccg aagagacaat gacttccgcg atccggttgg
      841 tcttttcttc catcacgccc atcatcacct gtgaaccata aatcagcagg atcaggtaga
      901 tcaggaatcc gcagacataa ccgattgctt cggcatattg ggcattggca ttttcatcct
      961 gaatgttttt gggcgtcacc atcaggcgct gctccagtat atcctgtttc agggagtcga
     1021 tctgcaggct gtcttttttt atcgccgtcc agatggaatt caatttggct tccaccgcgg
     1081 cagtggaact cctgccatag gccttattgg ctttaaattg taatgcggcc ccttgtttcc
     1141 agtcaatcgc cggtaccacg atataaccgt caaacccggc tttcttatga tcagcgatga
     1201 ccgaatctgc attccctttt accagggtga tcatggaatt gttatcggcc aggttttctt
     1261 tactaagcaa gggcgctgta aagtaaccgg atgaatcaat cagggccaca tgcaggctct
     1321 gcttactctt ctcggctata aatcccatgc cgaagatgag tgccaggtac agcaggggaa
     1381 acagcagggt ggaaataata aatgtttttt tgcgtacccg ggtcaggtat tcgcggcgta
     1441 tgatgatata tatcttattc attttgttgg tttgaatggt gatgattaag cttgaacccg
     1501 ttgaaactgg cgggctgtgg gcgtgccttc caccagtttg ataaagatgt cattcaggga
     1561 aggcaggatc tcattgaagg ccgtaatatt tcctccctga tcgagcaggt accggagtac
     1621 atcattgggg ctgctgccat ctttgatacg gatcacatga cggtcttcca tatgcccggt
     1681 taattcaaag ggggctgtag ccggcacact ggtcagcggt tggttcagtc ctacactgaa
     1741 aatattttcc ttgaattcct gttttaccgc tttgaccgta ccatccagta ttttctttcc
     1801 cttattcacc aggacaatat gattacagat ttcttccacc tgttccatcc ggtgtgtact
     1861 gaagatgatt gttgatccgc cctgggccag gttgaaaatt tcatccttga tcaggttact
     1921 gttgaccgga tcgagcccgc tgaagggttc atccaggatg atcagtttgg gttcgtgcaa
     1981 tacggtggtc acaaactgaa gtttttgtcc catccccttg gacaggtcct ccactttttt
     2041 attccaccag ctgtccattt caaaacgggc aaaccatttt tttattttcg actgtgcatc
     2101 actgtgactc agtcccttca gctgggccag gtacatggct tgttcaccga ttttcatttt
     2161 tttatacaga ccgcgttctt ccggcatata accgatcagg cccacatcat taacggggtc
     2221 gaattttttg ccatcaaaaa ttatctgtcc ttcatccgga tagaaaatcc cggtgatcat
     2281 ccttatcagg gtggtcttgc cagccccgtt gggtccgagc agtccgaata tctgtcctct
     2341 ttccacgttc aggctgatat catccaccgc cttctgcgtg gcgaagtatt ttttcaggtt
     2401 ctggagttca agtatggcca taatggttgg ttttgaagtg taaaattagt tttctgtacg
     2461 acttccccct ttattttcgg cgaactgcat caataagggc gtaaaatgct gccagatcca
     2521 cctgatcggt ggaaaagact tgcgtaaatc tcccgcaaag attaaaagcg tttactaaag
     2581 agggagctgt cttattttat cagacagttc gcttatggag gcgggtggta ttcaggataa
     2641 gtcttattgg ctttgcgggc tgtattttgc caggatcatg gcagctaccc gttcgccatc
     2701 catggcggca ctcacgatgc cccctgcata accggcccct tctccgcagg gatacagatt
     2761 ctctatctgg ggatgttgca ggttatccgc attccggggt atccgtaccg gtgaggaggt
     2821 gcgggattcg gtggccacca ccacggcctc attggtaata taacccttca tcttttttcc
     2881 gaattcacga aaaccaagac gcaggcgttc cgaaacaaag gccggtaata cctctttcat
     2941 ggaaacggaa tggattccgg gtaaataaga gcaatccggg agactccctg atattttatt
     3001 ttctgtaaag tcagctaacc gctgtgcagg agccaccatt ttacctccgc cggtcagaaa
     3061 ggctgtttgc tcaacggatt gctggaaata catcgctgcc agcgggccat atttattaaa
     3121 gccctgcacc tttttcttct atactgacta cgattccact gttggcatac gggttattcc
     3181 gtttggacgg gctccatccg ttgaccacca gttcaccggg agcagtggca gcgggagcaa
     3241 tgatgccacc ggggcacata caaaaagaaa agaccccttt accatccacc tgttgcaccc
     3301 aactgtaaga agcggggggg aggaactccc cccggaggtc acaatgatat tgggcactgt
     3361 ccaccaggga ttggggatgt tccaccctta cacccagggc aaagggtttt aattcaatga
     3421 gtattttctt atgatgaagt aattcaaaaa tatcccgggc cgagtggccg gttgccagga
     3481 tcacggcatc gccttcgaag cgctgtccgt ccgccgtctt tactgctttt attttttccc
     3541 cttccagtat aaaatccgtc actctttttt caaagaggaa ttcgcctccg caggccagga
     3601 ttttttcacg catagccgta atgatatgcg gaagtttatt ggtaccgata tgcggatgcg
     3661 actcatacaa tattttttca tcggcgccga agtgtacaaa caattccagg atccggttga
     3721 tatccccgcg tttgctgctg cgggtataga gtttaccatc actgtaagtg ccggcacctc
     3781 cttccccaaa acaataattg ctgtccggat ccaccactcc ctctttattg agcattgcca
     3841 ggtcacggcg gcgggctctc acatcctttc ctctttcgag cagcaggggc cggatgccgg
     3901 attcaatgag ttgtaaggcc gcgaaaagtc cagcagggcc ggctccgaca ataatgactg
     3961 tttgtgttgc tttatgtaca tcccggaacg aaaacgattt cctttccctt tcacgacatg
     4021 gctcgtttat ataggccagc agggtcaggt taacccaggg ttggcgtcca cgtgaatcaa
     4081 tggattgttt ttgtagtata tatccgctta ttgctgctgg ttttaccccg gcagccaggg
     4141 caacagactg tttcacgagt gtttcactcg cagcttcaga aggcaataaa cggagggtga
     4201 aggtcttttg cataacgtca ggtttttatc ctgaaagcgg gtacaaagat gcaaataaaa
     4261 acccgggata tttttttcct ttttcccgca tcgtatataa aattgtgata ccttcaaacg
     4321 agatcgtttg ttatgaaccc caggtattat ttttttccgg ctctgctttt attcttaaca
     4381 ggaatttcct gtcatacatc atacgagggc cagtcacttc aatataaagg ataccggatc
     4441 agccaaacca tgccggctga taccgctgta ttacatttcc tgcagccatt tagtgatagt
     4501 gtcaataaca gtatgaattc cgtattgggt gtaacatcgg agagtatgga gaaagcccag
     4561 ccggaaagca gtcttggtaa ttttatggcg gatgcgtttc tgatcatggc ccgggagaaa
     4621 taccaaacgc aggtggacct ggcttttgta aattttgggg gaatacgact gacacaatta
     4681 ccggcgggca atatcacccg tggtaaggtt tttgaactga tgccttttga taatctcctg
     4741 atccttcaaa aaatcaaagg gagtgtgctg caacaggtgc ttgacctgat ggcgggccgt
     4801 ggcggatggc cggtggctgg tatccggatg cagatcagta ataacaaggc cgtgaatgtg
     4861 caggttggcg gacagccgct ggatcctgaa aaagtttata ccattgctaa ttcagatttt
     4921 attgcgaatg gcggggataa tgcggatatg cttcgtccgg tgccacaaat tacgaatggc
     4981 tacctgatgc gggatgcgat tattgattat atcgcctggc tgaacagtaa tgggaaaaaa
     5041 attaccgcca cgattgaaaa ccgggtgaca catgctcagt agaaaaagat tttttaaaca
     5101 ggcggcgttg acagccgggg gcttatttgc cggaccagct ttgctggaag cggcagaata
     5161 tttcaatcca ctccggctca cgatcctgca tacaaacgat acgcatagcc ggcttgaacc
     5221 ctttccgatg gatgggggca gaaaccaggg aatgggtggg attgccgccc gtgcccggct
     5281 gatcagccag gtaagacagg aagaggaaca ggtattatta ctggatgccg gggatatttt
     5341 tcagggaacc ccttatttca atatatataa aggtgaacct gagatcaaag ccatgacctt
     5401 gctggggtat gatgcctgca cgattggaaa tcatgatttt gatgccggga tggaaaacct
     5461 ggctgcccaa ctgaccaaac atgcccgttt cccgatgctg gtcagtaatt atgattttag
     5521 cggaaccccg atggagaaca agacggaacc gtaccgggtg atccggaaag gcaaactaac
     5581 agttggtttg ttgggagtgg gcattgaagg taaaggattg atacccgata acctgtttgg
     5641 caatacgcaa tacctcgatc ccgtgcagaa agccaatgag acggctgaac tactgcgaaa
     5701 gaaaaaaaac tgcgatatgg tcatttgcct gtcgcacctg ggaaaccagt ataaaggaag
     5761 tgacaaagtg agtgacgagg tgctggccaa agagagtgaa aatattgacc tgatcattgg
     5821 gggacatacg catacctttc tggatcagcc gctggtgtat aagaataaaa agggggatga
     5881 ggtggtggtc aatcaggtgg gatttgccgg cctgattctt ggtagactag attttgaatt
     5941 taccaaattt tcgggcaaaa aactggcgaa aagtcacaca atttctgttt cacaaaaatc
     6001 aggtggataa aatttttttt taggaaagaa atagtgatat tcgcctccct gtacaacttt
     6061 tttaactgaa ctgtcgagaa gatttttagc ggagggattc aggaacaact aatgttaacc
     6121 cgacacaaac tgcttacaga ctgatggaga aaaatgctga taaaatctgg tcgaattgtt
     6181 tgaagatcat caaggatatt gtcgaatggc agcatttcaa gacctggttc gaacctatta
     6241 aaccggtttc cataaagaat aatgtcctcg tcatacaggt acccagccaa ttcttttttg
     6301 aatacctgga agaacattac gtaaatttat tagcgaagac actgaagaga gaactgggaa
     6361 aagaagcgcg gttggaatat cggatcatgg tggacagcgg caacagcaag aataagccgg
     6421 ttaccatgga tgtcagtggt cagggattta aaactttttc aaataacgaa atggattttc
     6481 cgctcgtcat aaacaatcct gttaagaacc cgtttgttat accgggtctt aagaagatgc
     6541 agattgatcc gcagctgaat ccgaactata cctttgaagc attcattgaa ggcgattgca
     6601 accgggtggc ccgtcgtgcc ggtaaaacgg tggcagaaaa gccaggcgca aattccttta
     6661 atccgctggt gatttatggc ggggtaggac tgggaaaaac ccacctggcc caggcgatcg
     6721 gaaatgaagt aaaaagactg catcccaaca aagtggtctt atatgtcagt tccgagaagt
     6781 tcatcaacca gtttatggac cacagccgga ataatgcgat caatgacttc atccattttt
     6841 accagctgat tgatgtcctg attattgatg atgtgcagtt ttttgccaaa gctgagaaat
     6901 cccaggatgc cttctttgcc attttcaacc acctgcacca atcccagaaa caactggtgc
     6961 tcacttccga taaatcaccc aaggacctgg acggggtaca ggagcgcctg ctcagccgtt
     7021 tccgctgggg ccttagtgcc gaccttcagg taccggaata tgatacccgg atcgagattc
     7081 tggagcggaa aatgaagaat gacggcctgg agatgcccaa ggaagtggta aaatacattg
     7141 cgtataacat cagtaacaat gtaagggagc tcgagggagc cctgatttca ctgttggcac
     7201 aatcttcgct gaacaggaga gagattgatt tagatctcgc taagaaagtg ttacggaact
     7261 ttgtaaaatc gtccagcaag gaaattacca tcgataccat ccagaaaatg gtttgcgagt
     7321 actttgatgt accttatgat aagctgttgc agaagacccg caaacgcgaa atcgtacagg
     7381 cccgccagat taccatgtac ctggccaaag cttttaccaa gaattcactg aaaacgattg
     7441 gggagcattt cggcggaagg gaccacacca cggtcatcca ctcctgccag accgtaaaag
     7501 acctgatgga taccgacggg gtattccggg agaatgtgct cgaactccag cagaaagtac
     7561 agctggccgc gatgtaaacg actcaacagc aagccaaaaa tattgttccc ggacacattt
     7621 atgtcgtccg ggtttttttt gaggggcacc ctgttcccgg tttaattttt ttgggatttg
     7681 ttcccggtat tttggattat aatcccggtt attcatattt
//
LOCUS       DAOPMW010000131         7656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10776_length_7656_cov_5.337982, whole genome shotgun sequence.
ACCESSION   DAOPMW010000131 DAOPMW010000000
VERSION     DAOPMW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10776_length_7656_cov_5.337982"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1356)
                     /locus_tag="PLU11_07690"
     CDS             complement(79..1356)
                     /locus_tag="PLU11_07690"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPN59037.1"
                     /translation="MKKIILGLLCWPIFLSAQIPDHIYRPNIRTVKLFKYGDIYSYPV
                     LQLGNNEQLELHFDDLDNDVKNYYYSFQLCNADWTPANVQTFDYIRGFQSNRIGTYRS
                     SSIVQTRYTHYQAILPERNSGPTKGGNYLLKVFLNDDTSRLVFSKRLLVVNKKASVAG
                     QVLQPFNGNMFQTHQRVQAGVSTAGTQLNPFSPQDLKLVILQNFNWGSASLIDRPTLF
                     RGNYYEYSDDAFTTFPATKEWRWADLRSFRLRSDRVQRIVDSDTSSRIDIYINPDMDR
                     RQQIYIYYRDINGLFSSENQDNGNAYWQADYAWVHFTYVPPGNQPIPGRQVYLFGELT
                     QYSPDEATRMEFNPEKGVYEKALYLKQGYYNYSYVTLPDRPQRGVPALPENAEGNYWG
                     TENSYMILVYFRPFGARADELVGYSLINSAFQR"
     gene            1455..2690
                     /locus_tag="PLU11_07695"
     CDS             1455..2690
                     /locus_tag="PLU11_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN59038.1"
                     /translation="MKVAGFIANRIAFNQQKTFSRFIIRLSIAATIISVMVMIVTLAL
                     ANGFQEAVSQKIFSFWGHIRVQEKQPGKAIISEEIAISEDTALVSTIARYPGVKSIHP
                     FATRYALLKTREDIEGVLVKGLDNTYDFEHLKQFIKSGRPIRFTDSLFSRELLVSDYT
                     ASRLQLQPGDSLLIYFLKPGENPRTKKMSIAGIYKTGIEEYDRLFAIGDIRLIRNLNQ
                     WNDDQAGGYEIFLNNYKLTDTIAIQLHELDSFPATWDAISVKKISPNIFDWLNMQDVT
                     RNVLISFMIAIAVINLVTCLLILVLERVRMIGILKSLGASDWTVQKVFLRHSMLITLT
                     GIVIGTVLALLLLWIQQETGFIKLKEEAYYLSSAAVKIVWWEVILVCGLTLLVSFLVM
                     LIPSILVRRIQPVKAIRFR"
     gene            complement(2683..3414)
                     /locus_tag="PLU11_07700"
     CDS             complement(2683..3414)
                     /locus_tag="PLU11_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPN59039.1"
                     /translation="MLGKSTIKYIQSLGQKKFRDEARVFIAEGPKIVSELIKACPGQV
                     KKVFALEEWLNEQQQLCRNIESEVISVEELERISQLKTPNKVLALINFFSNESIQLEG
                     RLSLGICGIQDPGNLGTIIRIADWFGVTQVICSEDSADLYNPKVIQSTMGSIARVNVF
                     YSDLGELLREMPGFPVFATVLDGMDVKTAGQPTQGIILIGNESKGIPGNLLDLSNNRI
                     TIPRKGGAESLNAAVAAGIILAHLT"
     gene            3497..5761
                     /locus_tag="PLU11_07705"
     CDS             3497..5761
                     /locus_tag="PLU11_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPN59040.1"
                     /translation="MITFFVSCSVIPKNYPKGKPFVFETKITVNGNFTNEEKEILTSR
                     LKGQLDDSMRARSVSKLFYSVMKNPPVYKKENADRSILYMRSLLNSLGYFHDSIYYKD
                     SIQLKTKEQQRAFISFFVNPGKVVRLDSISYNLKEAEIQSITLANKNESLLKKGDPFA
                     KTAISMELDRLVDLYRNNGYLRFNREELKGVWDTLDIKLLNPSLDPFEQLQILQQLKE
                     KRENPTANIEIILRPGFDTSKLVKYYVGNITIHPEYSPDTAGYTRKVEWVDGIQVISY
                     RKAFRSKILPVHVYLHRGDLYSQKNYYKTINRFNSLGAWRLSNIEQITRKGQDTADFI
                     IRLTPAKKYSFNANIETSQNQNQYLGNLFSIGTNVGITNRNFARSAIQTNSNIRYGVE
                     LGNNFVQSQQFVLSHTIYFPKAMLPRFGWIPEADRNNIRTVFGFNAARTERRELFNLT
                     SVNASWGYEYQYVKPGSHKLTQISIKLPNIEYSKLDPKDSLDKLIAANPTLKNVFTDG
                     FISSVILGTSLTNTHPRKPSVFSANLELPFLAGLIPSDFLDTQLYKFMKINAEYTQQF
                     KRKNSSFVFRAFAGIGYAFNSTVKESKRNALPFFKQYFGGGPNSMRAWRLRRLGPGSA
                     IKDYNEYPYRFGDIQLEMNMEYRFKITTIAGTRIESVLFTDIGNVWFMKKEAGDPEEV
                     FKLSRLGKDIAIGIGTGLRLDFTYFIIRLDYAYKVKDPSPDLQNQISQNKWFYNWKPL
                     NGQLQLGINYPFKL"
     gene            complement(5751..6443)
                     /gene="truB"
                     /locus_tag="PLU11_07710"
     CDS             complement(5751..6443)
                     /gene="truB"
                     /locus_tag="PLU11_07710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPN59041.1"
                     /translation="MENPINIFEEGQVLLIDKPLEWTSFDAVRKIRNLVRIKKVGHAG
                     TLDPLATGLLIVCTGKFTKRINEFMAQEKEYTGTFTLGAVTPTYDLESEPVSQRPYDH
                     ITSEMIQAATVPFLGEIYQVPPAHSAIKVAGKRVYELARAGKEVKLEPRKLFIHAFEI
                     TRIELPVVEFRVVCSTGTYIRSLAHDFGAALGCGAYLSKLCRTRIGEFSLQSAESIAG
                     FEQRVKALNATV"
     gene            complement(6674..>7656)
                     /locus_tag="PLU11_07715"
     CDS             complement(6674..>7656)
                     /locus_tag="PLU11_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPN59042.1"
                     /translation="DQPAIVDYTLRPETRGEYTFHDINVFVHAPLKLVRRRFVFEQEQ
                     VVKVYPSYFQMRRYQLLAMSNRLQDAGVKKIRKLGHSMEFEQIKEYVRGDDYRTINWK
                     ASARKQDLMVNNYTDERSQQIYCLINKGRVMKMPFHGMTLLDYAINASLVLSNVALVR
                     QDKAGLITFEKNLDTFVLADKKPTQMNQVLETLYKQKTDFREADFEKLFSVIRNRITN
                     RSLLVLFTNFESMESLQRELPALRKIAKYHLLLVIFFENTELKTVLEGKTRSLEDIYI
                     KTIAEKFVFEKKLMVKELHKNGIPSILTTPEGLTVNTVNKYLELKTRMSI"
BASE COUNT         2101 a         1781 c         1688 g         2086 t
ORIGIN      
        1 ggagctggtc gtgtaagacc tgccggcgca aagataaggc agtgcaaccg aatgactcgt
       61 atagaattgg aaaaaatttc agcgttggaa agcagaattg atcaggctgt agcccactaa
      121 ttcatcggcc cttgcaccaa atgggcggaa atagaccagg atcatataac tgttctctgt
      181 tccccaataa ttgccttccg cattttccgg aagagccgga actccgcgct gcggcctgtc
      241 aggcagggtt acataggaat aattataata tccttgtttc agataaagtg ctttttcata
      301 gacccctttt tccggattaa attccatcct tgtggcctca tcaggtgaat actgcgtcag
      361 ttctccaaaa agatacacct gtctgcccgg tatgggctgg ttgccgggtg gcacataggt
      421 gaaatgcacc caggcatagt ctgcctgcca gtacgcattg ccattatcct ggttctctga
      481 ggaaaacaag ccattgatat cccggtaata aatataaatc tgctgacgcc ggtccatatc
      541 cggattgatg taaatatcaa tacgggaaga ggtatcgctg tccacaatcc gctgtacccg
      601 gtcacttctc aaacgaaaac tacgaaggtc tgcccatcgc cattcttttg tggcaggaaa
      661 tgtggtgaaa gcatcatcac tgtattcata ataattaccc ctaaataaag tgggccggtc
      721 gatcagactg gcactacccc aattaaaatt ttgcaaaatg accagtttca aatcctgtgg
      781 cgaaaacgga ttaagctggg tccctgccgt actgaccccc gcctgtaccc gttgatgggt
      841 ctgaaacata ttgccattaa acggctgcaa tacctggccg gctaccgaag ccttcttatt
      901 gactaccagg agccgtttgg agaaaaccaa tctggatgta tcatcattca ggaatacttt
      961 caacagataa ttcccgccct ttgtgggccc ggaattccgt tcaggaagta tggcctgata
     1021 atgtgtataa cgggtttgaa cgatcgaaga acttcggtaa gtcccgatcc ggttactctg
     1081 aaaaccccgt atataatcaa acgtctgcac attggccggt gtccagtccg cattacataa
     1141 ctgaaaactg taataataat ttttgacatc attatctaaa tcatcaaaat gcaattccaa
     1201 ctgctcatta tttcccaatt gcagtaccgg ataactgtaa atatcaccgt acttaaacag
     1261 tttcacggtc cgtatgttgg gacggtaaat atgatcgggg atctgggccg acaggaagat
     1321 tggccagcaa agcagcccta aaattatttt tttcatgagc gggttgttct ctgaaaatat
     1381 tcgttgaaaa tacgttaaaa aaaaggataa atcctgagca gcaccttatt tttgataaag
     1441 acaaactatt atcattgaag gttgcaggat tcatagctaa ccgcatcgca tttaatcagc
     1501 aaaagacctt ttccaggttt atcatccgct tgtcaatagc ggccacgatc atcagtgtca
     1561 tggtgatgat cgttaccctg gccctcgcaa atggcttcca ggaagcggtc agccagaaaa
     1621 ttttcagttt ctgggggcat atccgtgtac aggaaaaaca acccgggaaa gctattattt
     1681 cggaagaaat tgctatttca gaagacaccg cattagtaag cacgattgcc cggtacccgg
     1741 gcgtgaagag tatacatccg tttgccacgc gttatgccct gcttaaaacc agggaggaca
     1801 ttgaaggtgt actggtaaaa ggactggaca acacctacga ctttgaacac cttaaacaat
     1861 ttataaaatc cgggcggccc atccgtttta cggacagttt gttcagccgc gaactgctcg
     1921 tctccgatta tacggcttcc cgactacagc ttcagccagg tgacagcctg cttatttatt
     1981 tccttaaacc cggtgaaaat ccacggacaa aaaaaatgag tattgccggt atttataaaa
     2041 ccggtattga agaatatgac cggcttttcg ccattggtga tatccggctg atccggaacc
     2101 tgaatcaatg gaatgatgac caggcaggtg gttatgaaat ttttctgaat aactataaac
     2161 ttactgatac cattgccata cagctgcatg aactggactc atttccggct acctgggacg
     2221 cgatcagtgt caaaaagatt tccccgaata tttttgactg gctcaatatg caggatgtta
     2281 cccgcaatgt actgatcagt tttatgattg ccatcgcggt gataaacctg gtcacctgcc
     2341 tgctgatcct cgtactggaa cgggtacgga tgatcggcat cctgaagtca ttgggggcga
     2401 gtgactggac cgtacaaaaa gtttttctcc ggcattccat gctgatcacc ctgactggca
     2461 ttgtgattgg gacggtgctc gcactgcttt tgttgtggat acagcaggaa accggtttta
     2521 tcaaattgaa agaagaagcg tattacctga gttcggccgc tgtgaaaatt gtttggtggg
     2581 aagttatatt ggtatgcggg ctgactttac tggtctcctt cctggtaatg ttgattccat
     2641 ccattctggt ccgtcgtatc cagcccgtaa aagcgatccg gttcaggtaa ggtgagccaa
     2701 aatgattccc gcagctacag cggcattcaa cgattcagct ccgcctttcc tcggaatggt
     2761 aatccggtta ttggataagt ccagtaaatt cccggggatt cccttcgatt cattgccgat
     2821 cagaatgata ccctgtgttg gttgtcctgc tgttttaaca tccattccat ccaacacggt
     2881 ggcaaatacc ggaaaaccgg gcatttcccg gagcaattct cccaggtcac tgtaaaatac
     2941 gttcacccgg gcaatacttc ccatggtgga ttgtatgact ttgggattat aaagatcagc
     3001 tgaatcttca ctgcaaataa cctgggtgac accaaaccag tccgctatcc ggataattgt
     3061 acccagattg cccggatcct gaataccaca tattccaaga ctcagccggc cttcgagctg
     3121 tatggactca ttactgaaaa aattaatcaa tgccagtacc ttattgggtg tctttaattg
     3181 tgaaattctt tctaattctt ctacagatat tacttcggac tctatattcc ggcacagctg
     3241 ctgttgttca ttcagccatt cttcaagggc aaataccttt ttcacctgcc cgggacaggc
     3301 ttttatcagc tcactaacta tcttgggacc ttcggcaata aagaccctgg cctcgtccct
     3361 gaattttttc tggcctaaac tttgaatata tttgattgtt gatttaccaa gcatagtcac
     3421 tcttttacca tttatgataa caggacagcc ttaccatcga agcaaactgc tgctgctttt
     3481 accggcactt tctgttatga ttaccttttt tgtttcctgc agcgtaattc ctaaaaatta
     3541 tccgaagggg aagccattcg tatttgagac aaagatcacg gtaaatggca atttcaccaa
     3601 cgaagaaaag gaaatcctga catcccggct gaaaggacag ctggatgaca gtatgcgggc
     3661 ccgttcagtg tccaagcttt tttacagtgt aatgaagaat cccccggtat ataaaaaaga
     3721 aaatgctgac cggtcgatcc tttatatgcg atcgcttctc aattccctcg gttactttca
     3781 cgattctatt tactataaag atagtattca gctcaagact aaagagcaac aaagagcctt
     3841 tatcagtttt tttgttaatc cggggaaagt cgtaagactg gattctatca gttataatct
     3901 aaaagaggct gaaatacaat ctatcaccct cgcgaataaa aacgaatccc tgctgaaaaa
     3961 aggggatccc tttgccaaaa ctgccatatc catggaactg gaccgcctgg tagacctgta
     4021 tcgcaataac ggttacctgc ggtttaaccg ggaggagtta aaaggtgttt gggataccct
     4081 tgatataaag ctgctgaatc ccagcctgga cccttttgaa cagttacaga tacttcagca
     4141 gctgaaagag aaaagggaga atccaacagc gaatatcgag atcatactgc gcccgggatt
     4201 tgataccagc aagctggtga agtattatgt cggaaatatc actattcatc ccgaatacag
     4261 tccggatact gccggatata cccgtaaagt ggaatgggtg gatggaatcc aggtaatttc
     4321 gtaccggaag gcattccgat caaagatcct gcctgtgcat gtttatttgc accggggtga
     4381 tctttacagt cagaaaaatt attacaaaac gatcaaccgg tttaattcat tgggcgcctg
     4441 gcggctgtct aatattgaac aaataacccg taagggtcag gacacggcag actttattat
     4501 tcggctgacc cctgccaaaa aatactcgtt caatgcgaat attgaaacaa gccagaatca
     4561 gaatcagtat ctcggcaacc tgttcagtat cggaacgaat gtgggtatta cgaaccggaa
     4621 ttttgccagg tcggccattc agaccaacag caatatccgg tatggagtgg aactgggaaa
     4681 caattttgta caatcccagc agtttgtgct gagtcatacg atctatttcc ctaaagcgat
     4741 gcttccccgt tttggctgga tcccggaagc agaccggaat aatatcagga cggtttttgg
     4801 ttttaacgcg gcaagaacag aaagacggga attattcaac ctgacttctg tcaatgcctc
     4861 ctggggttat gagtaccagt acgtgaaacc cggcagccat aaactgacgc agatttcaat
     4921 aaaactgccc aatattgagt actccaagct ggatccgaag gacagtcttg ataaactcat
     4981 tgcggcgaat cccacactca agaacgtatt cacggatggc tttatttctt cggtcatact
     5041 tggcacatcg cttacaaaca cccatccacg taaaccttct gtattttccg caaacctgga
     5101 gttgcctttc ctggcgggcc ttatccccag cgattttctg gatacccagt tgtataaatt
     5161 catgaagata aatgctgaat atacgcagca gttcaagcgt aagaattcct cttttgtatt
     5221 cagggctttt gctggtatcg gatatgcgtt caattccact gttaaagaat ccaagagaaa
     5281 cgccctcccc ttctttaagc aatatttcgg cggcgggccc aacagtatgc gtgcctggcg
     5341 tctccgccgg ctcggcccgg gctctgccat caaagactac aatgaatacc cctaccgctt
     5401 cggggatatc cagctggaga tgaatatgga gtaccggttc aagattacga ctatagctgg
     5461 caccagaatc gaaagtgttt tattcactga tatcgggaat gtatggttca tgaaaaaaga
     5521 agcaggtgat ccggaagaag tattcaaatt aagccggctg ggaaaagata ttgcgattgg
     5581 tataggaacc ggcctccggc ttgatttcac gtacttcatc atccgtctgg attatgccta
     5641 taaagtaaaa gatccgagtc cggatctgca gaatcaaatc agtcagaata aatggtttta
     5701 taactggaaa ccgctcaacg ggcagttaca gcttggcatc aattatccgt ttaaactgta
     5761 gcattcaggg ctttcacccg ttgctcaaag ccggcaattg actcggctga ctgaagggaa
     5821 aattcaccga ttcttgtccg gcaaagttta ctgaggtagg caccgcatcc cagggccgct
     5881 ccaaaatcat gggccagact acgaatatag gtgcccgttg aacacacgac acggaattcc
     5941 acgacgggta attcaatccg ggtgatctca aatgcatgaa tgaataactt acggggctca
     6001 agtttcactt cctttccggc cctggccagt tcatacactc gtttgccagc gactttgatt
     6061 gccgaatgtg caggtggcac ctggtaaatt tctccaagaa aaggaactgt agccgcctgt
     6121 atcatttcag aggtgatatg atcatagggc cgctgactga cgggttcaga ttcgagatca
     6181 tatgtgggtg tgaccgcccc cagggtaaat gttccggtat actctttttc ctgcgccata
     6241 aactcattga tgcgtttggt aaacttaccg gtacatacaa tcagcaaacc ggtggccagc
     6301 ggatcaagtg ttcctgcatg ccccactttc tttatacgca ccaggttcct gatcttacgc
     6361 actgcatcaa aagaggtcca ctccagcggt ttatcgatca gcagcacctg accttcttcg
     6421 aatatattaa tgggattctc cataaataca aaagtacgaa gacaagtacg aagtacgagg
     6481 tacgaagtac gatcctaaag atcgggatta actaaaggat gggaaagccg tttaaaaata
     6541 agaggtacga agtatgaact taaagttcgg aattaactaa agtatggaga agtcgttcag
     6601 actgctaatg taccggggag taaaaagcac ccgtaaaaca accacttcgt acttcgtacc
     6661 tcgtacttcg tacttaaatc gacattctgg ttttcagttc caggtatttg ttaaccgtgt
     6721 ttacagtcag tccctccggg gtggtaagga tggacggaat accgtttttg tgtagttctt
     6781 ttaccatgag ttttttctca aagacaaatt tctctgcaat ggttttgatg tagatatctt
     6841 ccagtgacct ggttttccct tccaatactg ttttcagttc tgtgttttca aaaaagatga
     6901 ccagcaggag gtgatacttg gcaattttcc ggagtgccgg caattcgcgt tgcagacttt
     6961 ccatggactc aaaattggta aaaaggacca gcaggctccg gttagtgatc cggttcctga
     7021 tcacggaaaa cagtttttcg aagtcagctt cccggaaatc ggtcttctgt ttataaagcg
     7081 tttccagcac ctggttcatt tgtgtgggtt tcttatcagc cagcacgaag gtgtccaggt
     7141 ttttctcaaa agtgataagt ccggctttat cctgccgcac cagtgccaca ttactcaata
     7201 ccagggaggc attgatcgca taatcaagga gggtcattcc atgaaacggc attttcatca
     7261 cccggccttt gttgatcaga caataaatct gctggctccg ctcatccgta taattattca
     7321 ccatcaggtc ctgcttacgg gcagaggctt tccagttgat agtacgataa tcatctcccc
     7381 gcacatattc tttgatctgt tcaaactcca tactatgtcc gagtttcctg atctttttaa
     7441 caccggcatc ctgcaggcgg ttgctcattg ccagcagctg gtaacgccgc atctgaaagt
     7501 aggacgggta tacttttact acctgttcct gttcgaaaac aaaacgtcgc ctgaccagtt
     7561 tcagtggcgc atgtacaaat acattaatgt cgtgaaacgt atattctccc ctggtttcag
     7621 gccgcaacgt atagtcaact atggctggct gatctg
//
LOCUS       DAOPMW010000132         7636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10814_length_7636_cov_6.907004, whole genome shotgun sequence.
ACCESSION   DAOPMW010000132 DAOPMW010000000
VERSION     DAOPMW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_7636_cov_6.907004"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1008)
                     /gene="truA"
                     /locus_tag="PLU11_07720"
     CDS             complement(262..1008)
                     /gene="truA"
                     /locus_tag="PLU11_07720"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPN59043.1"
                     /translation="MSRYFLEIAYKGTDYSGFQSQENAVTIQSEVEKAIAVLQREPVS
                     LTGSSRTDAGVHALQNYFHFDFDGTLHPQFVYKLNAILPRDIVLHKLIAKGAEAHCRF
                     DAVSREYKYFIYRSKDPFLQDRAYYFPYHIDLERMQAAACILKEYEDFTSFSKRNTQV
                     KTFKCRLELSEWVEEGPCLVYRVKGNRFLRGMVRALTATMLKVGRGKINLDEFRSIIE
                     ARDCTKASFAVPPHGLFLIRVNYPVGYFDQ"
     gene            complement(1070..2578)
                     /locus_tag="PLU11_07725"
     CDS             complement(1070..2578)
                     /locus_tag="PLU11_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59044.1"
                     /translation="MKRFIFLIVLAVFFMTASAQKVYFMYLQAEPAQPFFLKLNGKVH
                     SSSASGYLILSRLVDTTYTITIGFIKPGIREQVFTVRTEQKDHGFLLKDFGEKGWGLF
                     DLQRLTVQYSETVPVAVQLQATAGKQVSPFTELLSKATGDPSLRENPPAPKAEEIKPV
                     VKPDAVAIEQPKTEPQMPVATEDPKQVAIVPVVETPKLITEKKEEVPPVKIEAIPAKA
                     EPIVVSKDEKKEEPVIVVKEPEIKSPVIPDTVTKASVVPPVVNETKKEEPKLAPDQEK
                     VIPVDNSPVKTEAVTEVLYKRSVVTKRSESSTTEGFGLVFLDTWEGGKIDTIRLLIPN
                     PKQVVALVKETPKVEKKFLEIQATATKPDEKTPVVEEKAKPAEPVKPVAEVKAEEQPV
                     VPADKPADKTVAAAPCAETATEADFFALRKSMAASEGDEEMISQAKKYFRQKCFTAAQ
                     LKNLSALFLNDEAKYNFFDAAYKYTADKAAFATLESELKDPYYINRFKAIIR"
     gene            2836..5352
                     /locus_tag="PLU11_07730"
     CDS             2836..5352
                     /locus_tag="PLU11_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPN59045.1"
                     /translation="MKKVLLSVLFLLAGITMMAQPKPPADTAWKKEYRETATRINNLV
                     HTKLEVKPDFSKSWLYGKAWITLKPHFYATDSLTLDAKGMEFKKITLLKGTKQLPLKY
                     TYNDWQLKIQLDKTYKASESYTVYIEYTAKPDEFEEKYAKGAMLGIKGMYFINPKGED
                     KNKPTQIWTQGETESSSAWFPTIDKTQQKTTQELTVTVDNKYVTLSNGKLMSQKKNAD
                     GTRSDYWKMDLPHSPYLFFLGVGDYAVIKDSWRGKEVNYYVEKEYASVAKKIFGNTPE
                     MMSFFSKITGVDFPWIKYSQITGRDYVAGAMENTTATIHQESAQQDARELVDGNNWEG
                     TIAHELFHQWFGDYVTAESWSNLTVNESFADYSQTLWDEYKYGKDAGDAENYQGMQGY
                     IMSGSDKKDLVRFHYKDREDMFDAVSYQKGGRILHMLRTFVGDSAFFKSLNNYLTTNK
                     FKSAEAHQLRLAFEEVTGRDLNWFFNQWYFGSGHPQVDISYQYNDTTGIANIIVKQTQ
                     KTKLFTLPLAIDVYTGAEKTRFNVWSKNAADTFSIPYKTRPDLINVDGDKVMLWVKKD
                     AKTLDNYIHQYKYAGKYMDRREAIDFAAKNQTNPKAVDLLKTALNDPYKGLRGFTISK
                     LDLKKDAVKKMAEPVLADMAKNEKAATVKASAIDRLAEFGKKEYTDLFKAAVNDSSYS
                     VAGNALNALISVDPEAAAPIVKKLSGTPMKGILQSVVMSEILKSGDESMADQIIGDFS
                     KMPLGQGKFEALNGMTTYLAAIKNTEKIKWGVDEVVKFREQVPENFRSQTDPFINSMV
                     LKGILAAKGKQLKETPDDKALAELVEYIKAKLPEADKKGF"
     gene            complement(5508..6269)
                     /locus_tag="PLU11_07735"
     CDS             complement(5508..6269)
                     /locus_tag="PLU11_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPN59046.1"
                     /translation="MKNLLLTISMILSIWASAQIDKIVPAKPNPPRLVNDFTGTLTDA
                     QRQTLENKLVAFDDSTSNQVAVVIIPTIGGYSVDEVALQIVRQWGVGIKDKNNGIVIL
                     VAKNDRKIKIEVGYGLEGAVPDVTAKHIIDEEIVPAFKENNYFRGLDKGTDAIIKASA
                     GKYKPSANYGSKKKGISTGMIIFIVIILILVFGGGGKGGTYVSRGGYRGFGGLGGFGG
                     GGWSGGGGWSGGGGGGGFGGFGGGSGGGGGASGSW"
     gene            complement(6275..6733)
                     /locus_tag="PLU11_07740"
     CDS             complement(6275..6733)
                     /locus_tag="PLU11_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPN59047.1"
                     /translation="MNFFFWKKKSLLDEESNRLIVKAIRHAEQRTSGEVRVFVESRCR
                     FMNALDRAQEIFFSLKMNQTEERNATLVYVAIKDRQVAVFGDEGIHQKVGVEYWDNVV
                     KEMLAQFNADNYAKAIGDAVIQIGDALQTHFPYDKETDKNELPDEIVFGR"
     gene            complement(6749..7327)
                     /locus_tag="PLU11_07745"
     CDS             complement(6749..7327)
                     /locus_tag="PLU11_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPN59048.1"
                     /translation="MKGTRNVFLLGIVALFLMLFVWGCNVRNGLASAETDIKGKWGLV
                     QTQYNRKAKLYENVVNTIKGAAKNEDTTLIKIVQMRSRVPNISEGSSPEQVAAADRQL
                     NAIGRSALNINIEAYPTLQATGLYRDLQAQVEGTENRVTVALNDWNNAITDYNQKIVR
                     FPSSMIAGWFGFKQKDNYKAPEGSEDTKVDFN"
BASE COUNT         2131 a         1841 c         1676 g         1988 t
ORIGIN      
        1 ttttgaaatg cagtactgat gcaggttttc agcgaaaaaa actttgaaat tttctgcccg
       61 aaccctcctt tttagggcat ttaccggact tcttacaccc gaaaatgggc aaatcagggc
      121 ttattcacag cactgtaaaa gttctgaatc gacgtccggc ccccgaaaaa aatcagtata
      181 aactactaca agtgagcccg atgactcttt tacagtaaag aaatgatcgg cggagaattc
      241 aaaaaaactg caggaaatgg cttactgatc aaaataaccc accggataat tgacccggat
      301 aagaaataac ccatgcggcg gtaccgcaaa actggctttc gtacaatccc gtgcctcaat
      361 aatcgatcgg aattcatcca gattgatctt accccgccca actttaagca tggtggcggt
      421 cagcgcccgg accatccccc ttaaaaaccg gttacctttt acccgatata ccaggcaagg
      481 gccttcctcc acccactcac tgagttccaa ccggcattta aaggtcttta cctgcgtatt
      541 ccgcttggaa aaagaagtga agtcttcata ctccttcagg atgcatgccg ctgcctgcat
      601 gcgctccaga tcgatgtggt aagggaaata gtaggcccgg tcctgcagga aggggtcctt
      661 ggaccgatag ataaagtact tgtattcgcg gctgactgca tcaaatcggc aatgtgcctc
      721 cgcccccttg gcgattagct tatgcaatac aatatcgcgg ggcaagatgg cattcagctt
      781 atagacaaac tgcggatgca acgtgccgtc aaagtcaaaa tgaaaataat tctgcaaagc
      841 atgtaccccg gcatcggtgc gggaggaacc cgtcagactg accggctccc gttgtaatac
      901 ggcgattgct ttctccactt cggattgtat cgttacggca ttttcctgtg actggaaccc
      961 actgtaatca gtgcctttgt atgctatttc caggaagtaa cgggacatgt aacagatagt
     1021 agttagtaga tagtagttag gagtcaacag ccttgaacct gttcaatact caacgtatga
     1081 tggccttgaa ccgattgata taatagggat cttttaattc ggattcaagg gtggcaaaag
     1141 cggctttgtc ggctgtgtat ttataggccg catcaaagaa attgtacttg gcctcatcgt
     1201 tgaggaataa agcactcaga ttcttcaact gcgcagccgt aaaacatttc tgcctgaaat
     1261 atttcttcgc ctggctgatc atctcctcat ccccttctga tgctgccatt gatttacgaa
     1321 gggcaaaaaa atcggcctca gtagctgtct ccgcacaggg agctgccgcc accgttttat
     1381 cagccggctt atccgccggt acaaccggtt gctcttctgc tttcacttcc gccaccggct
     1441 taacgggttc tgcgggtttt gctttttctt caaccaccgg agttttttcg tccggcttgg
     1501 ttgctgtagc ctgaatttct aaaaacttct tttcaacttt aggtgtttct ttcaccaggg
     1561 ctaccacctg cttcggattt ggaatcagta aacgaatagt atcaatcttc cctccttccc
     1621 atgtatccaa aaacaccaaa ccaaaccctt cggtggttga actctctgat cgtttggtta
     1681 cgactgaacg tttatacagc acttctgtca cagcttcggt ttttaccggg ctattatcca
     1741 cgggaattac tttttcctga tcaggtgcca atttcggttc ttcttttttt gtttcattga
     1801 caaccggggg aacaaccgat gcttttgtta cagtatcagg aatgacgggt gattttattt
     1861 ccggttcctt cacgacaatg accggctcct ccttcttttc atccttacta acaacaatcg
     1921 gttcggcttt tgccggaatg gcttcaattt ttaccggggg aacttcttct tttttctcag
     1981 tgattaattt gggagtttcc accaccggga caattgccac ctgcttcggg tcctcggttg
     2041 caactggcat ttggggttct gttttaggct gttctatggc caccgcatct ggtttgacca
     2101 ccggctttat ctcctctgct ttgggtgccg gaggattttc ccgcagagag ggatctccgg
     2161 tggctttgga taaaagttca gtaaatggag aaacttgttt tcccgccgtt gcctgtagct
     2221 gtacggccac tggaactgtt tctgaatact gtactgtcag gcgctgcaag tcaaaaagtc
     2281 cccacccctt ttctccaaaa tccttcaaca gaaatccatg atccttctgt tccgtcctga
     2341 ccgtaaatac ctgttcgcga atgcctggtt taataaaacc aattgtgata gtataagtag
     2401 tatcaaccaa acgagataga atcaggtagc cgctggcaga tgaagaatgt actttcccat
     2461 tcagctttaa aaaaaatggc tgcgcaggtt ctgcctgcag gtacataaaa tacactttct
     2521 gggcagatgc ggtcataaaa aaaaccgcca gcactatcaa aaagataaat cgtttcatac
     2581 aacctgattg gttcgtaaca aatttaactg aattggggga atattaggga atggtaaata
     2641 gtaaatggaa aataggaaat acagagtagc ctgtccttcc cgcctgtcta ccgtagtttt
     2701 aacgcaggta ggaagcgaag tgagggagag gggcggaaca caaggacttg ttaaagctgg
     2761 taaccttcca tccaaaaagc atgtctgatg gcaggcgaat cacctaattt tgccgcctaa
     2821 attcaactat tatttatgaa gaaagtattg ctttccgttc tttttctcct ggctggaatt
     2881 accatgatgg cacagcccaa accaccggct gatacggcct ggaaaaaaga ataccgggaa
     2941 acggccaccc ggatcaataa tctggtgcat accaaactgg aagtaaagcc ggacttcagc
     3001 aaatcatggc tttatggaaa agcctggatc accctgaagc cccatttcta cgccaccgac
     3061 tcgctgaccc tcgatgcaaa aggaatggag tttaaaaaaa tcaccctgct gaaagggacg
     3121 aaacaattgc cccttaaata cacctataat gactggcaac tgaaaattca actggataaa
     3181 acctacaaag ccagtgaatc ctatacggta tatatagagt acacggccaa gccggatgag
     3241 tttgaagaaa agtatgctaa gggcgccatg ctgggcatca aaggcatgta ttttatcaat
     3301 cccaaaggag aagataaaaa caaacccacc cagatctgga cacagggtga aactgaatcc
     3361 agttccgcct ggttccctac gatagataaa acccagcaaa aaaccaccca ggaactgaca
     3421 gtaaccgtag ataataaata cgtgaccctg agtaatggta aactgatgtc acaaaagaaa
     3481 aatgcggatg gcacccggag cgattactgg aaaatggacc tgccccactc cccttacctg
     3541 ttcttcctcg gggtgggtga ttatgcagta attaaagaca gctggagagg caaggaagta
     3601 aattactatg tggaaaagga atatgcctcc gttgcgaaga agatctttgg taacaccccg
     3661 gaaatgatga gcttcttttc aaaaattacc ggcgtggatt tcccctggat taaatattcc
     3721 cagatcaccg gacgtgatta tgtggcaggt gctatggaaa ataccaccgc caccattcac
     3781 caggaaagtg cccagcagga tgcgcgggaa ctggtggatg gcaataactg ggaaggaacc
     3841 attgcgcacg agctttttca ccagtggttc ggtgactatg taaccgcaga aagctggagt
     3901 aacctgaccg ttaacgagtc ctttgcggat tacagccaga ccctgtggga cgaatacaaa
     3961 tatggaaaag atgccggaga tgctgaaaat taccagggca tgcaaggcta cattatgagc
     4021 ggcagtgata aaaaagacct ggtccgtttc cattataagg accgggaaga tatgtttgat
     4081 gcggtgagct accagaaagg tggacgtatc ctgcatatgc tgcgcacttt tgtaggggac
     4141 agtgctttct tcaaatcgct gaacaattac ctgacgacga ataaattcaa atctgcggaa
     4201 gcacaccagc tgcgcctggc ctttgaagaa gtaaccggac gtgacctgaa ctggttcttt
     4261 aaccaatggt atttcggcag cggacatccg caggtggata ttagttacca atacaacgat
     4321 accacgggta ttgccaacat aatcgtaaag caaacccaga aaaccaaact cttcaccctg
     4381 cccctggcca ttgatgtcta taccggtgcg gagaaaaccc gttttaatgt atggtcaaaa
     4441 aatgcggccg atacattcag catcccatac aagacccgtc cggacctgat caatgtggat
     4501 ggcgataaag tgatgttatg ggtaaagaaa gacgccaaga ccctggataa ttatattcac
     4561 caatataaat atgccggtaa atatatggat cgccgcgaag ccattgattt tgccgcaaag
     4621 aaccagacga atccaaaagc agttgatctg ctgaaaaccg cgctgaatga cccttataaa
     4681 ggacttcgcg gatttaccat cagtaaactg gacctgaaga aagacgccgt aaaaaaaatg
     4741 gcagagcctg tactggctga tatggcaaaa aatgaaaaag cagctaccgt aaaagcgtcg
     4801 gcgattgacc gcctggctga atttggcaag aaagaatata cagacctgtt caaagcggcg
     4861 gtaaatgact cgtcctactc tgtggccggg aatgccctga atgcattgat cagtgttgac
     4921 cctgaagccg ccgcgccaat tgtaaagaaa ctctccggga cacccatgaa aggaatttta
     4981 cagtctgtgg taatgagtga gattctcaag tcaggggatg aatcaatggc cgatcagatc
     5041 atcggtgatt tctcaaaaat gccactcgga cagggaaaat ttgaagcctt gaacggcatg
     5101 accacttacc tggccgcgat aaaaaataca gaaaagataa aatggggtgt ggatgaagtg
     5161 gtgaagttcc gcgaacaagt gccggaaaac ttccgcagtc agaccgatcc ttttatcaac
     5221 agcatggtgc tgaaaggcat cctggccgcc aagggcaaac aattaaagga aacaccggat
     5281 gataaagcgc tggctgaact ggtggaatat atcaaggcca agttgcctga ggcggataag
     5341 aaaggattct gaggaagtag tgagtagtga aatgtcatta aagtaaagtg ccctccgaaa
     5401 atcggagggc actcttattt gtaaagtatt ttttattcta tcaattgata aatattctta
     5461 agaggggctg ttgactacta actaccgact actttctacc gactagctta ccagctgccg
     5521 ctggcaccac caccgcctcc gcttccccca ccaaagcctc caaaaccacc gccaccaccg
     5581 ccaccggacc atccaccacc gcctgaccaa ccaccaccgc caaaacctcc taatccgcca
     5641 aatccgcgat acccgccacg ggagacatag gttcccccct tcccaccacc gccaaatacc
     5701 aggatcagta taatgacaat aaagattatc atacctgttg aaatgccctt cttcttggaa
     5761 ccgtagtttg ccgacggttt atacttgcct gcggacgctt tgataatggc atctgttccc
     5821 ttatctaaac cccggaaata attattttcc ttgaatgcag gaacgatctc ctcatcaatt
     5881 atatgttttg ccgtaacatc cggcactgca ccttccaaac catagcctac ttcgatcttt
     5941 atttttcggt cgttcttcgc taccaggatc acgatcccgt tattcttatc cttaatacca
     6001 acgccccact gcctgacaat ctgcaaagcc acttcatcta ccgaatagcc ccctatggtg
     6061 ggtattatga ccaccgccac ctgattggag gtgctatcgt caaaagcgac tagtttgttt
     6121 tccagggtct gacgttgtgc atcagtcaag gtgccagtaa aatcgtttac cagcctgggg
     6181 ggattgggtt ttgcaggtac gatcttatca atctgagcgg atgcccagat tgataagatc
     6241 atagaaattg tcagtaggag attcttcatg ttgattaacg gccaaacacg atttcgtcgg
     6301 gcagctcatt tttatcggtt tccttatcat aagggaaatg cgtttgcaaa gcatccccta
     6361 tctggatcac ggcatcacca atggctttcg cataattatc agcgttaaac tgtgccagca
     6421 tttccttcac tacattgtcc caatattcaa cgcccacttt ttgatggata ccttcgtctc
     6481 caaagaccgc aacctgccgg tccttaatgg cgacatatac caatgtagcg ttacgttctt
     6541 cagtctggtt catcttaagg ctgaaaaata tctcctgggc ccggtcaagt gcattcatga
     6601 aacgacagcg gctttccaca aaaaccctga cctcaccgct tgtgcgttgt tcggcatgcc
     6661 tgatcgcttt gacgataagc cggttgcttt cctcgtctaa aagagatttc ttcttccaga
     6721 aaaaaaagtt cactaagttt tattttaatt agttgaaatc aactttagtg tcttcagacc
     6781 cttctggcgc tttatagttg tctttttgtt tgaagccaaa ccaaccggcg atcatgctgg
     6841 aagggaatcg tacaattttc tgattgtagt ctgtgatcgc attgttccag tcattcaaag
     6901 ccactgttac acggttctct gtaccttcca cctgggcctg caggtcccta taaagccctg
     6961 tagcctgtaa tgtcgggtaa gcttcaatgt tgatattcag tgcagagcga ccgatcgcat
     7021 tcagctggcg gtcagctgct gctacttgtt cggggcttga accctcacta atattcggta
     7081 cacgggaacg catctgtaca attttgatca gcgtcgtgtc ttcgttttta gccgctcctt
     7141 tgattgtgtt taccacgttc tcataaagct tggctttccg attgtactgg gtctgcacca
     7201 aaccccattt gcctttaata tccgtttctg cggaggctaa gccattccgg acattacagc
     7261 cccatacgaa taacatcaga aacaatgcaa ctattccaag taagaataca ttacgggttc
     7321 ctttcatttt actgagtttt tgatttgata ctaaaattag tgcttttggg aaatacagga
     7381 ggggattttc ggtaagtaaa actagattgc cggtaaaagg ccctgcgggg gcggcaaaga
     7441 gttcaaatga ggagaaaaaa gagaaaagag ggcgtgatta ggttaaagcc actcaaagat
     7501 ttaaacgctg aaacttctaa aacacttagc caccgcgggt tggtaaacga ctctccaaat
     7561 taattaaggc ctctcattta actctttttc tcctttgacc tctttgccaa aaaaaacctc
     7621 cccaaaagga aggttt
//
LOCUS       DAOPMW010000133         7592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_10905_length_7592_cov_7.747247, whole genome shotgun sequence.
ACCESSION   DAOPMW010000133 DAOPMW010000000
VERSION     DAOPMW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10905_length_7592_cov_7.747247"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PLU11_07750"
     CDS             <1..815
                     /locus_tag="PLU11_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN59049.1"
                     /translation="GFRAPTLAELASNGTHEGTNRYEYGSNELTSETSLQLDGGIDLD
                     YEHFSLGLSAFYNRINDFIFYRKLESLSGGDSLVNVDGEDILAFKFNQHNAQLSGLEF
                     TLDIHPHPIHWLHFENNFSFVRGRFDDLLDGFKPGSDNLPLIPAPRWTSEIRADFQKA
                     GKSIRNFYARLEGDQTFKQDRFFSGYETETATNGYFLLNAGLGADITNTKKKTIFSLH
                     FGVMNIGNVAWQNHLSRLKYSAENLVTGRTGVFNAGRNFSVKLNVPLEFKLK"
     gene            922..1395
                     /locus_tag="PLU11_07755"
     CDS             922..1395
                     /locus_tag="PLU11_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HPN59050.1"
                     /translation="MEISLTTPALLFPAISLLLLAYTNRFLALANLVRKLHDEYIEGR
                     KSHDEFTELHKNKIILWQIKNLRRRINLIRYMQGLGVFSFLGCVGCMYSLYRGAAVVA
                     EFIFAVALLSLLASLFISLAEIIQSTKAIELELSDIEELEKGNIFTDIISGTKEK"
     gene            1452..1859
                     /locus_tag="PLU11_07760"
     CDS             1452..1859
                     /locus_tag="PLU11_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59051.1"
                     /translation="MAGFLAACSKSGGSTDEGAGGGGPHIVTDNDTTRPVLTVMTPLN
                     NQVFANGSSVSVTGSITDDMGLYRGYIRITNDVTGEVVKEQLYEIHGILSYNFNVSAA
                     VTVTAATDYTVSVFYEDHGLNGVSRTMKVKVNP"
     gene            complement(1860..2168)
                     /locus_tag="PLU11_07765"
     CDS             complement(1860..2168)
                     /locus_tag="PLU11_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59052.1"
                     /translation="MGLFSFLSNGSLKKALRQGAVIIDVRSASEYDRGRVPGSLNIPV
                     DRIGASIPRIKGLNKPVICVCASGHRSRIAADTLKQAGLKEVYNGGSWERILRLIESL
                     "
     gene            complement(2178..4028)
                     /locus_tag="PLU11_07770"
     CDS             complement(2178..4028)
                     /locus_tag="PLU11_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59053.1"
                     /translation="MKILFKYLRPYKWLVGLVLLLAAVNIGFSLVDPIIFGRLINLAT
                     YHQNKIAFSWDSFLFFNESITEEVIKVKPGGIVEKVMETRHYYGVAWLLLASITVAMI
                     SRIAKAFQDYFLNLITQKFGATVFTEGLQHAMKLPYQEFEDARSGETLSVLQKVRLDT
                     EKFVSSFINVLFPVIVGILFVAVYATTIHWSLPLVYFGGIFLLTIISNLLSKKVKTIQ
                     KKIVGQTTALAGATTESLRNIELVKSLGLTQQEVSRLNKNTFKILGLELTKVKRIRSI
                     SFIQGTFVNTLRQIILFLLMLFIYRGSMDAGQIVTMQIFSFFVFGPLQEIGNIILTYR
                     EAQASLENFANLMKKSPEPRAANPSHLGRIETLEFDHVAFKHQTASQNAINDISFKVK
                     TGETIAFVGPSGSGKTTLMKLLVGLYRPQDGKILYNGMDENSIDFEDLRNQIGFVTQD
                     TQLFSGSIRENLLFVNPSATEEDLEDVLKKASCQNLLSRAEKGLDTMIGEGGLKLSGG
                     EKQRLSIARALLRKPKLLLFDEATSALDSLTEEEITDTIRDISKEGSQVTILIAHRLS
                     TIMHADRIYVLEKGDVVETGNHELLIGEKGLYFAMWRQQIGERKSRAAVV"
     gene            4312..5487
                     /locus_tag="PLU11_07775"
     CDS             4312..5487
                     /locus_tag="PLU11_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59054.1"
                     /translation="MRHKKPNRRQFLQDVSKASGLMMLAPMTGNFTGIGPSYVEAPVI
                     PAGKIKFAVININHPHIYGMTEAIKRGGGELVALYAIEADLSAAFLKAYPEARMAKNE
                     AEILEDPSIHLVLSSGIPDERAPLGIRVMQHGKDYLTDKPGIITLKQLAAVKKVQKET
                     KRIYSIMYSERLENKGTEKASQLVKAGAIGQVIQTINLAPHRINNNIGKTGLAVRPDW
                     FWDKNRFGGILTDIGSHQFDQYLHFTGTTEARIVASQIGNLHHPDKPLFEDFGDVMLS
                     GNGGLGYIRVDWFTPDGLNSWGDGRLTIMGTEGYIEVRKNTDIVSAHKGGNHLYLVNK
                     KEMLHMDCNNEILPFGPRFVDDVLNRTETAMSQAHCFLATELALKAQKNATVLSLRK"
     gene            5532..6959
                     /locus_tag="PLU11_07780"
     CDS             5532..6959
                     /locus_tag="PLU11_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59055.1"
                     /translation="MKKKKQRNHPAAESKEKNLNRRKFLNDSVKAALGTGLALSFPTI
                     VPASVFGKYAPGNWINVAAIGVGRISRGHDIPGVWKQDFARVMAVCDVDSRRAADGQQ
                     LVNEYYAKKEGKPFDGVKIYSDYRELLLNKDIDAVLISTPDHSHAMIGVAAARAGKHI
                     YMQKPASLTIAEGRVMSDVIRKTGVKFQIGSQQRSAEQFRYAAELVRNGRIGELKNVY
                     VGLPGDPPGGNKTEMPVPAGFDYNMWLGSTPEAYYTEERVHPQKDYGRPGWLRCEQFG
                     AGMITGWGSHHIDSAHWGMGMEGSGPVEVWCEKVEFNTGGLWDVHGVFRTGARYNNGV
                     SMSVSNEYPNGIKFEGTQGWIFVSRGDYQATSSDPSKPGEQAKKLDASDPKLLTSVIG
                     EKEFHFTVSNDHHGNWLEAIRDNKATIAPVEEAHRSCSACLLHHISMKLKRKLQWNPE
                     QEAFVNDAEATAMLSREQRKGFEVK"
     gene            complement(6967..>7592)
                     /locus_tag="PLU11_07785"
     CDS             complement(6967..>7592)
                     /locus_tag="PLU11_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59056.1"
                     /translation="DELPEFKRTVLEVMRQPMEERRVTISRAKVAVDFPASFMLMASM
                     NPCPCGYYNHPDRVCSCGPGMVQKYLNKISGPLLDRIDLHVEVTPVAFSELSAIRPAE
                     PSCAIRDRVIKAREIQAERYKENPGVYCNAQMSSKQLKEICVINAVGANLLKAAMQKL
                     NLSARAYDRILKVSRTIADLCDSPDIKPEHLAEAIHYRSLDREGWGG"
BASE COUNT         2022 a         1818 c         1830 g         1922 t
ORIGIN      
        1 gtggcttccg ggcacctact ttagcggaac tggccagtaa cggaacacac gagggcacca
       61 accggtatga gtatggttcc aatgaactga cctcagaaac cagtctgcaa ctggatggag
      121 gaatcgatct ggattatgaa catttcagcc tgggtttgtc ggccttttat aaccgcatta
      181 atgattttat tttttaccgg aaactggaat ccctctccgg aggtgattca ctggtgaatg
      241 tggatggaga agatatactg gcctttaaat tcaatcagca caatgcacaa ttatccggcc
      301 tggagtttac actggatatc cacccgcacc ccattcattg gctgcatttt gaaaacaact
      361 tctcctttgt aaggggccgc tttgatgatt tactcgacgg cttcaaaccc ggcagtgata
      421 acctgcccct gatacccgcc ccgcggtgga ccagcgaaat aagggccgac tttcaaaaag
      481 ccggtaaatc aatccgtaat ttttatgccc ggctggaagg ggatcagacc tttaaacagg
      541 accggttttt tagtggttac gaaacggaaa cagccaccaa tggatacttt ctcctgaatg
      601 caggtcttgg tgcagatatt acgaatacta aaaagaaaac aatcttcagt ctccatttcg
      661 gggtaatgaa tatcggtaac gtggcctggc aaaatcacct cagccggttg aaatattccg
      721 ccgaaaacct ggtaacaggc agaaccgggg tatttaatgc cggccgcaat ttctccgtca
      781 agctgaatgt accgctggag tttaagttga agtaagcagc gagggaggga agttcgaggt
      841 tggaggttgg aagttcgagg ttcgaacctc caacctccaa cctcgaacct cgaactgccg
      901 acttttcccg acctttatcc tatggaaatc tccctgacca ccccggcact cctgttcccg
      961 gccatctccc tgttgttgct ggcctatacc aatcgttttc tggccctggc caacctggta
     1021 cgcaaattac acgatgagta tatagagggt cgtaaatccc atgatgagtt tacggagttg
     1081 cacaaaaaca agattatcct ctggcagatc aaaaatctgc gtcgccggat caacctgatc
     1141 cgttatatgc agggcctggg ggtattcagt ttccttggtt gtgtgggttg tatgtacagt
     1201 ttgtaccggg gagcagcggt agtagcggag tttatttttg cagtagccct gctgagtttg
     1261 ctggcttcct tgtttatttc cctggcagaa attatccaga gcaccaaggc catcgaactg
     1321 gaactaagtg atattgagga actggaaaag gggaatattt ttacagatat tatctctggt
     1381 accaaagaga aataatacga tatatttgat ttatgcagaa accgggacaa agtattttca
     1441 tattccttat catggccgga tttctggccg cctgctccaa atccggcggc agtactgatg
     1501 aaggcgctgg gggtggaggt ccgcatattg tgacggataa tgataccacc cggccggtgc
     1561 tcacggtgat gaccccgttg aataaccagg tttttgcgaa tggttcttca gtcagtgtaa
     1621 ccggatctat cactgatgat atgggtttat accggggcta tatccgtatt accaatgatg
     1681 tgacgggcga ggttgtgaaa gaacaacttt acgaaattca tggaattctg tcgtataact
     1741 tcaatgtatc tgcagcggtt acggtaaccg ctgcaaccga ttatacggta agtgtgtttt
     1801 atgaagatca tgggctgaac ggggtcagcc gcacgatgaa agtaaaagtc aatccctgat
     1861 cagagcgatt caattaaacg aagtatccgc tcccagcttc ccccattgta gacttctttc
     1921 aagccggcct gtttcagtgt atccgcggct atccggctcc ggtgaccact ggcgcaaaca
     1981 caaatcaccg gtttattcag tcctttaata cgtggtatac tcgccccaat gcggtcaacc
     2041 ggtatgttca gtgaaccggg aacccgtccc cggtcatatt cactggcaga tcgtacatca
     2101 atgattaccg ctccctgtct taacgctttt tttaaggagc cattactcaa aaaagaaaaa
     2161 agtcccatac tgtaaattta aacaacggcc gcccgggatt ttcgttcccc gatctgctgc
     2221 cgccacatcg caaaatacaa tcccttttcg ccaatcaata actcgtgatt cccggtctcc
     2281 accacatcgc ctttctcgag cacatagatc cggtccgcat gcatgattgt actcagccgg
     2341 tgagcaatga ggatggtcac ctggctgcct tcttttgaaa tatcccggat cgtatctgta
     2401 atctcttctt cggtcagcga atcaagggct gaagtggctt catcaaacaa taacaattta
     2461 ggctttcgta ataaagcacg ggcaatggat agacgctgtt tttcaccacc ggacaacttt
     2521 aatcctcctt ccccgatcat cgtatccagt cctttttccg cccgggataa gaggttctga
     2581 caggacgctt tttttaatac atcttccaga tcctcttctg tcgccgaggg attcacaaat
     2641 aataagttct cgcggatcga gccggaaaac aactgggtat cctgggtaac aaagccgatc
     2701 tgattgcgca ggtcttcgaa gtcaatactg ttttcatcca tcccattata caggatcttt
     2761 ccatcctgtg gccggtacaa acccaccagt aatttcatta aagtggtctt gccggaaccg
     2821 gaggggccca caaaggcaat cgtttccccg gttttcactt tgaagctgat atcattgatc
     2881 gcattctgcg aagcggtctg gtgtttaaaa gccacatggt caaattccag tgtctcgatc
     2941 cgtcctaaat gactcggatt ggccgccctg ggttccggtg attttttcat caggttggca
     3001 aaattctcca gtgaagcctg ggcctcgcga taggtgagaa tgatattgcc aatctcctgc
     3061 agcggaccaa acacaaagaa agagaagatc tgcatcgtca cgatctgccc ggcatccata
     3121 ctgccgcggt agataaacaa catcagcagg aataatatga tctggcgaag tgtattgaca
     3181 aacgtaccct ggatgaaact gatactgcgg attcttttaa ccttggtcag ttccagcccg
     3241 agtatcttaa aggtattttt attgagccgg gatacttcct gctgggtcaa accgagactt
     3301 ttcaccagtt caatattccg cagggattca gtagtggctc ctgccagcgc cgtggtttgt
     3361 cccacgatct ttttctggat ggtctttact tttttactca gcaaattcga aataatggtc
     3421 agcaggaaaa tgccgccaaa ataaaccagg ggcagactcc agtgaatcgt ggtggcatac
     3481 acggctacaa ataaaattcc cacgatcacc gggaaaagca cattaataaa agaagaaaca
     3541 aatttttcgg tatccagcct tactttctgt agtacactta aggtttcccc gctccgcgca
     3601 tcttcaaatt cctgataggg gagtttcatc gcatgctgta atccttcagt aaatacggtg
     3661 gctccgaatt tttgcgtgat caggttcagg aaataatcct ggaaggcttt ggcaatccgg
     3721 ctgatcatcg ccacggtaat ggaagccagc aacagccagg ccaccccata ataatgacgg
     3781 gtttccatga ctttttccac gatcccgcct ggttttacct tgatcacctc ttcggtaatg
     3841 ctttcgttga agaacaggaa cgaatcccag ctgaatgcaa tcttattctg gtggtatgta
     3901 gccagattga tcagccgacc gaaaataatg ggatccacga gggagaaccc gatattcacg
     3961 gcagccagga gtaaaaccag ccctaccagc catttatatg gcctgaggta tttaaataat
     4021 atcttcataa atgggattga ggcgaaaaat ggaaatgcag attcaatgta caaacttcgt
     4081 acaagtttag gaaatatgcc aagttaacag gccccgggct cttttctctt cttttttagt
     4141 catttagcac cctccagaat ccaatcaaac agctgttcag tctgtcggtt gctgttttca
     4201 tccgggaaaa ccgggcaacc cccggatagt ggttcaatca aaaatagtta catttatttt
     4261 catccgggat tgtacaacat tccggtgtcc taattcaaaa aaacgatttg catgcgccac
     4321 aaaaagccca atcggagaca gtttttacag gacgtcagca aagcatccgg attaatgatg
     4381 ctggcaccca tgacaggtaa ttttaccggg atcggtcctt cctatgtgga agcccccgtg
     4441 atccctgccg gcaaaataaa attcgctgtc attaatatca accatccgca tatttatgga
     4501 atgacagaag ccataaaaag aggaggaggc gaactggtgg ccttatatgc catagaagca
     4561 gacctgtcag ctgccttttt aaaagcttat cccgaagcca ggatggcaaa gaatgaagcg
     4621 gagatcctcg aagatccgtc catacacctg gtattgagct cgggcattcc cgatgaacgg
     4681 gctccactgg ggatccgggt tatgcagcat ggcaaagatt atttaaccga taagcccggc
     4741 atcattacgc ttaaacaact ggcagcagta aaaaaagtgc agaaagaaac gaagcggatc
     4801 tattcaatta tgtacagtga gcggctggaa aataaaggca cggaaaaagc cagtcagctg
     4861 gtaaaagcag gtgccattgg ccaggtaata caaacgatca atttagcacc acaccggatc
     4921 aataataata ttggaaaaac cggcctggct gtacgaccgg attggttctg ggacaagaat
     4981 cgttttggcg gtattttaac cgatatcggt tcccatcagt ttgaccaata cctgcatttt
     5041 accggaacca cagaagcccg gattgtggct tcacagattg gaaacctgca ccaccccgat
     5101 aaacctttat tcgaggattt tggcgatgta atgttgagtg gaaacggtgg attaggttat
     5161 atcagagtcg attggtttac accggacgga ctcaattcct ggggcgatgg ccgcctgacc
     5221 attatgggca cagaaggtta tattgaagta cgcaagaaca cggatatcgt atcggcgcat
     5281 aaaggtggta accatttata cctggtgaat aaaaaggaaa tgctgcacat ggattgtaat
     5341 aatgagatac tgcctttcgg tccccgtttc gtggatgatg tgctgaaccg taccgaaaca
     5401 gccatgtcgc aagcacactg cttcctcgca accgagctgg ccctgaaagc tcagaagaat
     5461 gcaacggtcc tttcattaag aaaataaccg gtttccgaac gtgttgaacc cggataaact
     5521 atttgccaca tatgaaaaag aaaaaacagc ggaatcatcc tgctgctgaa agcaaagaaa
     5581 aaaacctgaa ccgccgtaaa ttcctgaatg actccgtcaa agcggcgctt ggtaccgggc
     5641 ttgccctgag tttccctact attgttccgg cttccgtttt tggtaaatat gcgcccggca
     5701 actggatcaa tgtggccgca atcggtgtcg gacgtatttc acgcggacat gatataccag
     5761 gtgtatggaa acaggatttt gcgcgggtga tggctgtctg tgatgtggat agccgtcgtg
     5821 ctgctgatgg ccaacaactg gtcaatgaat attatgccaa aaaagaaggg aagccctttg
     5881 atggggtgaa aatctattct gattaccggg agttattatt gaacaaggat attgatgccg
     5941 tattgatcag tacccccgat cattcacatg cgatgatcgg cgtggcggcc gcacgggcgg
     6001 gtaaacatat ttatatgcag aagccagctt cgctgaccat tgcagaagga cgtgtgatgt
     6061 cggatgtgat ccgcaaaacc ggtgtgaaat tccagatcgg cagtcagcaa cgcagtgcgg
     6121 aacaattcag gtatgcggcg gaactggtcc gcaatggacg gatcggtgaa ctgaaaaatg
     6181 tatatgtagg actaccgggt gatccgccgg ggggaaataa aacagagatg ccggtacccg
     6241 ccggttttga ttataatatg tggctgggtt ctactcccga agcctactac accgaagaac
     6301 gcgtgcatcc gcaaaaggat tatggtcgtc ctggctggct ccgatgcgaa cagtttgggg
     6361 cgggcatgat caccggctgg ggctcacacc atatcgacag cgctcactgg ggaatgggca
     6421 tggagggcag cgggcctgta gaggtctggt gtgagaaagt ggaattcaat accggcggcc
     6481 tctgggatgt gcatggtgtt ttcaggaccg gcgcccggta taataacggg gtgagcatgt
     6541 ccgttagtaa tgaatatccc aacgggatta aatttgaggg tacccaaggt tggatatttg
     6601 tgtcccgcgg cgattaccag gccaccagca gtgacccctc aaaacctggt gaacaggcaa
     6661 agaaactgga tgccagtgac ccgaagctgc tgacatcagt catcggggaa aaggaatttc
     6721 attttactgt cagcaatgac caccatggca actggcttga agccatccgc gataataaag
     6781 ccaccattgc cccggtggag gaagcccatc gttcttgttc cgcttgttta ttgcatcata
     6841 tttccatgaa actgaaacgg aagttgcagt ggaatccgga gcaggaagca tttgtgaatg
     6901 atgcggaagc caccgccatg ctaagccggg aacaacgaaa agggtttgaa gtaaaataag
     6961 tattcttcag ccgccccaac cttccctgtc cagactgcgg taatggatgg cttctgccag
     7021 gtgctcgggt tttatatccg gactgtcaca caggtccgcg atcgtacggc tcactttcag
     7081 gatccggtca tacgcccggg ctgagagatt gagtttctgc atggccgctt taagcaggtt
     7141 agcacccacc gcattaatca cacagatttc tttcagttgt ttactgctca tttgtgcatt
     7201 gcaatacacg cctggatttt ctttataacg ttccgcctgt atttcccttg ctttaatcac
     7261 ccggtcacgg atcgcgcaag aaggttcggc cggccggatg gcggaaagtt cgctgaaagc
     7321 caccggcgtg acttccacat gcaggtcaat ccggtcgagc aagggaccgg aaattttatt
     7381 taaatatttc tgcaccatac cgggaccgca actgcatacc cggtccgggt gattataata
     7441 cccgcagggg catggattca tggaagccat cagcataaaa gaagccggga aatccaccgc
     7501 tactttcgct cttgagatcg taacccttct ttcttccatc ggctgccgca tcacttccag
     7561 aactgtccgt ttaaattcag gcaactcatc ca
//
LOCUS       DAOPMW010000134         7521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11050_length_7521_cov_8.952317, whole genome shotgun sequence.
ACCESSION   DAOPMW010000134 DAOPMW010000000
VERSION     DAOPMW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11050_length_7521_cov_8.952317"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLU11_07790"
     CDS             <1..287
                     /locus_tag="PLU11_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPN59057.1"
                     /translation="GLFVEGYEKNPVLLNYGIDELRFTKPVYPGAEVHIRFTCKEKLP
                     NDKRVIEKPEDFKRGDDIDKGIVKWLVEFFDASNEITGVATILTMVARKP"
     gene            660..1415
                     /locus_tag="PLU11_07795"
     CDS             660..1415
                     /locus_tag="PLU11_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPN59058.1"
                     /translation="MDAFVKSSFHNGITSIEFFHPQSNSLPGRILDELAQAIHAAGND
                     EATKVVLIRSFGDKAFCAGASFDELAAIRSKEEGLKFFSGFAQVINAMRICPKLIIGR
                     IHGKCVGGGVGLAAAVDYAIATDAAEVKLSELAVGIGPFVVGPVVERKIGNSAFSQLA
                     IDATRWRSAEWARKRGLYAELYNTVEDMDEAISRLAYLLVQSSPEAMAELKKVFWQGT
                     EHWDQLLVERAAISGRLVLSDFTRNAIESFKKK"
     gene            1415..2050
                     /locus_tag="PLU11_07800"
     CDS             1415..2050
                     /locus_tag="PLU11_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPN59059.1"
                     /translation="MQQFNNGNFHLLCDKLARKDHDLKGIIREYGYPPMWTRPATFQT
                     LILTILEQQVSLASAYAAFKKLREKIGYVTPAKILALTDEEMRAVYFTRQKMGYAREL
                     ARAIHSRKLVLKKLEPLHDEEVRAQLIQLKGIGHWTIDVYLMHALQRTDLFPLGDIAL
                     VNSLKLIKKLPPDISKEEMLKIAEPWRPNRTIAAMLLWHAYIQRKGIVISQ"
     gene            2308..3264
                     /locus_tag="PLU11_07805"
     CDS             2308..3264
                     /locus_tag="PLU11_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN59060.1"
                     /translation="MELKNIFRKSALVMALGLPVLLTSCDKDEDPVSNTITDVVVNNA
                     SFSTLEAAVLKANLQTTLSGTGPFTVFAPNDAAFTASNFTSATLNSLSADSVSKILLY
                     HTIPARILAADVPAGPNAKVVTASGDSVFVTKNSNGVFINGIQVTSADVTADNGVIHV
                     IGRVLNPPIGNIVETAVAARLDSLVKAVTRATTAPGGDPTLASTLSTARLTVFAPTNA
                     AFTQLLSALSLTDINQIPIGTLLAVLRYHVVGARAFSSDLANGNLTMLAGGNTTINLT
                     NGTGGGPSIQGNGNGGNRSNITATNIVCRNGVVHLIDRVLLP"
     gene            3353..4381
                     /locus_tag="PLU11_07810"
     CDS             3353..4381
                     /locus_tag="PLU11_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN59061.1"
                     /translation="MFNHRYRYLFILLLALYTFFNTVVCDVYHYFRIEIEWYYAALTI
                     LSVTLVTWEGNRLIKPWFKKKFLTPKTKVRFLAFFFIAGNGVALLAAIGTVFFFGTVI
                     HDYSWQENLNPYKLNIIYGSLINLFLHLLNAIHFFFREYRRQWKETEELRQISAQAQI
                     QLVKSQINPHFLFNNLNVLSGMVIKDNPEANRFIEEFSKVYRYILSNQEKELVELRSE
                     LDFIQPYIFLLQTRFDQGLSVTVNIPEHYKSWHVVPAALQMLIENAIKHNVVSRNKPL
                     HIDIHTNGNQTLVIKNNVQPRLAVESSTRIGLQNIRKRYELICGRDVVIKQSEKAFEV
                     AIPLLQLN"
     gene            4386..5138
                     /locus_tag="PLU11_07815"
     CDS             4386..5138
                     /locus_tag="PLU11_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN59062.1"
                     /translation="MKLIIVEDEQLATERLKTMLRKYDPAIEVIACLESIEDTVQYLK
                     THSHPDLLLLDIHLSDGHSFEIFKQVNYSGPVVFTTAYDIYALEAFKMLSIDYILKPV
                     TQDALAAALNKFKSLSLSFNAAQLRSQLPSIQEQRYKKRFLGKIGQRLFFIDTNEIAF
                     FQAENKIVYLIDKSGTRYIVEHTMEKLEDQLDPRQFFRLNRKFIVSIQAIQQVKPYFN
                     SRLKLSVLGAPQQEEMVISRDRVAEFRQWAEY"
     gene            5379..6860
                     /locus_tag="PLU11_07820"
     CDS             5379..6860
                     /locus_tag="PLU11_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPN59063.1"
                     /translation="MSTSLLLLIIVIYFSLLLVISRFTSRQSRDNTFFDGDRQSPWFL
                     VAFGMIGSGISAVSLVSIPGNVGNNHLYYFQFILGSIAGYLFIAFVLLPIFYKLRVVS
                     LYQYLESRFGPVSYKTGALFFIVSQSFGAALRLLLSVKILQYAFFDELGIPYAVTIAV
                     VLLLIWLYTNKSGVKTIVWTDALQSLFLITVILVSIISIKNALNLSIGDMFSSIASHP
                     YSTIFDWDPQSGSNFFKQFISGLLITVALVGLDQSMMQKTLTIKDAAGARKNVLSFSF
                     FIAAAQTLFLGLGVLMYLYTEQKGIPLLKENGQILNTDGIYPDLTLHYFGKTGAIAFF
                     IAVIASTFASIDSCITALTTSFSYDFMDFDKKSPAERRQIKNRVLLGVNLVMFIIVLA
                     FWNSQGAIINTIFKIAGYTYGPLLGLYLTGFFTSYKLKEKWVPLACIGAALLTYALNE
                     LFTRYYQFDLGFMNIGLNAGLTIVFLYLLKRKMNDQYKEYSNP"
     gene            6829..7308
                     /locus_tag="PLU11_07825"
     CDS             6829..7308
                     /locus_tag="PLU11_07825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPN59064.1"
                     /translation="MINTRNIVIRECHDPLVFDMCADLMAATDPWITLGMDKARCRLG
                     FEGLGKEVHVAWNDEMIAGFTILQTAGSFRGYIQTLCAAPIFRGSGVGTRLLHYCEER
                     VSAYSPNLFICVSEFNKGALKLYTNFGFKQVGSIPDFVKEGYTELLLRKTRGPLVKG"
BASE COUNT         1944 a         1727 c         1752 g         2098 t
ORIGIN      
        1 cagggttatt tgtagagggc tatgaaaaaa atccggtgct gctgaattac gggattgatg
       61 aactgcgttt caccaaaccg gtgtacccgg gtgcggaagt gcatatccgc tttacctgca
      121 aggagaaatt gccgaatgat aaaagggtga ttgagaagcc ggaggatttt aagcgtgggg
      181 atgatattga taaggggatt gtgaaatggc tggtggagtt ttttgatgcc tccaacgaga
      241 ttacgggcgt agccacgata ttgaccatgg tggccaggaa gccttaggct agtcggtagt
      301 caggagtcgg tagtcaggag tcaatacacc ccctgagaaa atatggctta atattcagca
      361 gattctcgtt ttacgaaatc tatccgctac tcacaacttg cttaaaatcc gtgcaaatcc
      421 gtgtgatccg tggcctgcct ttctgtattt ctgtattctt tcgtgccctt tcgtggcaat
      481 tcgtggccac cccatttgcc gcaagcaaaa aaatccgtgt aaatccgtgt gatccgtggc
      541 aaccttgccg aaggcaaaat ttgcgcagca aatactttcg cttttattcg tgtgaattcg
      601 tggccaaaaa accgcaaagc ggccctccat ggcttaactt tactacagaa accttcgtta
      661 tggacgcatt tgtaaaatca tcttttcaca acggaattac cagcattgaa ttctttcatc
      721 cgcaaagtaa ttcactgcca ggccggatac tggatgaact ggcccaggct attcatgccg
      781 cgggtaatga tgaagcaacc aaagtggtac tgatccggag tttcggggat aaagcttttt
      841 gtgccggcgc ttcgtttgat gaactggccg ctatccgcag caaagaagaa ggattgaaat
      901 tcttcagtgg ttttgcccag gtgatcaatg ccatgcgcat ttgccctaaa ctgatcatcg
      961 gccggataca tggtaaatgt gtaggcggtg gtgtcggact ggccgcagct gtggattatg
     1021 ccattgcaac ggatgcagct gaagttaagc tcagcgaatt agcggtgggg atcggaccct
     1081 ttgtagtcgg acccgtcgtg gaaagaaaga ttggcaactc cgccttctct cagctggcta
     1141 tagatgccac ccgttggcgc agtgctgaat gggcacgtaa aagaggctta tacgccgaac
     1201 tctacaatac cgtggaagat atggatgaag ccatcagtcg gctggcctac ctgttggtac
     1261 aatccagtcc ggaagccatg gctgaactga aaaaagtttt ctggcaaggc accgaacact
     1321 gggatcagtt gcttgttgaa agggcggcaa tcagcggacg tctcgtactg agcgatttta
     1381 cccgcaacgc cattgaatct tttaagaaga aatagtgcag caattcaata acggcaattt
     1441 tcacctcctc tgcgataaac tggcgcgtaa agaccatgat ctgaaaggga tcatccggga
     1501 atatggatat ccccccatgt ggacccgtcc ggccaccttt caaaccctga tccttaccat
     1561 acttgagcaa caggtttccc tcgcatctgc gtatgccgca tttaaaaaac tgcgggaaaa
     1621 aattggttat gtgaccccag ctaaaatact ggctttgacc gatgaggaaa tgagagccgt
     1681 ttattttacc cgtcagaaaa tgggctatgc ccgcgaactg gccagggcta ttcattcccg
     1741 caaactggta ctgaaaaaac tcgaacccct gcacgatgag gaggtaagag cccaactgat
     1801 ccaattgaaa ggcataggac attggaccat tgatgtgtac cttatgcatg cccttcaacg
     1861 taccgatctt tttcccctcg gtgatattgc cctcgtgaat agcctcaaac tcatcaaaaa
     1921 acttcctccg gatatttcca aagaggagat gctgaaaatt gccgaaccct ggcgacccaa
     1981 ccggaccatc gcggccatgt tgttgtggca tgcctatatt caacggaaag gtattgtaat
     2041 cagtcagtag ttggcagcag gcagtcggca gtcaacagcc cctcctgagg aaagattggt
     2101 ggaattggtt cttataggaa tgtggagtct gtaaaacctt cctgatctga ggatttttta
     2161 ctttttactt ttgatttttg acttctccgt taaacaaccc tactccctcc atactacgat
     2221 tcactccggc tttctgccga ttcactcctt cctgcttatc tttttctccc gggtgctgtt
     2281 agtatgatta aacaaaataa atcaactatg gaactcaaaa acattttccg taaatctgcc
     2341 ctggtaatgg cccttggctt accggtattg ctgaccagct gtgataaaga cgaagatccg
     2401 gtaagcaata cgataaccga tgtagtagtg aataatgcct ctttcagtac cctggaagca
     2461 gctgtgttga aggccaatct ccagaccacc ctgagtggta caggtccctt tactgtattt
     2521 gctcccaatg acgccgcttt tacggcctct aatttcacat ctgccacact caacagttta
     2581 agtgcggata gcgtatccaa gatcttatta taccatacaa ttcctgcccg gatactggcc
     2641 gctgatgtac cggctggacc aaatgctaaa gtggtgactg ccagtggtga ctcagtattt
     2701 gtgaccaaga acagcaatgg tgtatttatt aacggtatcc aggttacttc tgcggatgta
     2761 actgctgata atggagttat ccatgtgata ggccgtgtgt tgaatccccc tattggaaat
     2821 attgtagaaa ctgcagtagc tgcccggctc gattcattgg taaaggcggt tacccgtgcc
     2881 accaccgccc ccggtggtga cccaaccctg gctagcacct tgagtactgc ccgtttgacg
     2941 gtatttgccc ctactaatgc cgctttcacc cagttactca gtgctttatc tctgacggat
     3001 attaatcaga taccgattgg caccttgctg gccgtattgc gttaccatgt ggtaggtgcc
     3061 cgtgcatttt cgtctgatct ggcaaatggt aacctgacca tgctggccgg tggaaatacc
     3121 accatcaacc tgaccaatgg aactggtggt ggtcccagca ttcaggggaa cggaaacggc
     3181 ggaaaccgct cgaatatcac cgctacgaac attgtgtgcc ggaacggtgt tgtacacctg
     3241 atcgatcgtg tgttgttgcc ctaaataagt cccaatccca catgactatg aaaagccatt
     3301 aactgccctg tttcaggttt gccggaacag ggcagcttta attttttaga aaatgttcaa
     3361 tcaccgatac cgatacctgt ttattctgtt gctggcactg tatacttttt ttaatacagt
     3421 tgtctgcgat gtgtatcatt atttccggat tgaaatcgag tggtactatg cggccctgac
     3481 cattctctcc gttaccctgg taacctggga gggaaaccgg ctgatcaagc cctggtttaa
     3541 gaaaaaattc ctgactccta agaccaaggt ccgtttcctg gccttttttt tcattgccgg
     3601 aaatggtgta gccctgcttg cagcgattgg tacggtattc tttttcggta cagtgataca
     3661 tgattacagc tggcaggaaa atctcaatcc gtataaactg aacattatct acggcagcct
     3721 gatcaatctt tttcttcacc tgctcaatgc cattcatttc tttttccggg aataccggcg
     3781 gcaatggaaa gaaacagaag aactccggca gatcagtgcc caggcgcaga tccaactggt
     3841 gaaaagtcag atcaatccgc attttctctt caataacctg aatgtgttgt ccggcatggt
     3901 gatcaaagac aatccggaag ccaaccggtt tattgaggag ttttccaaag tatatcgtta
     3961 tatactcagt aaccaggaga aggaactggt ggagcttcgc tccgaacttg attttataca
     4021 accgtatatt ttccttctcc agacccgttt tgatcagggt ttatcggtaa ccgtgaatat
     4081 tccggagcac tacaagagct ggcatgtggt tccggctgcg ttgcaaatgc tgatagaaaa
     4141 cgcaataaaa cacaatgtgg tcagccggaa taagccattg catattgata ttcataccaa
     4201 tggaaatcag acactcgtaa tcaaaaataa cgttcagcca cgattggctg ttgaatcctc
     4261 tacccggatt ggcctgcaaa atatccggaa gcgatatgaa ctaatttgcg gcagggatgt
     4321 tgtgattaag caatcggaaa aggcgtttga agttgccatc cccctcctgc aattaaactg
     4381 aaacaatgaa gctgatcatc gtagaggatg aacaactggc cacggaaagg ctgaagacca
     4441 tgctcaggaa atatgatcct gccattgagg tcattgcctg cctggagagt attgaagata
     4501 ccgtgcaata cctcaaaacg catagccatc cggatctcct gctgcttgat attcacctgt
     4561 ccgatggaca tagtttcgaa attttcaaac aggtaaacta tagtggtccg gtggtattta
     4621 ccaccgctta cgatatttat gcgctggaag catttaagat gctcagcatc gattatatcc
     4681 tgaaacccgt gacacaggat gcattagctg cagccctcaa taaattcaaa tcgctttctc
     4741 tgtctttcaa tgccgctcaa cttcgttcac agttaccctc gatccaggaa caacggtata
     4801 aaaaacggtt cctcggtaaa atcggacagc ggcttttctt tatagatacc aatgagatcg
     4861 cattcttcca ggcagaaaac aagattgttt acctcataga taaatccggc acccggtata
     4921 ttgtggaaca taccatggaa aaactggaag atcaactcga tccccgtcag ttctttcgtc
     4981 tcaaccggaa attcatcgtc agcattcagg ccatccagca ggtaaagccc tacttcaaca
     5041 gccggttaaa actttcagta ttaggtgccc ctcagcaaga ggaaatggtc atcagccggg
     5101 acagggtggc ggagttcagg caatgggcgg agtactagtt agtagttagt agtcgggagt
     5161 cgggagtcaa cagccagctg ccagtcggca gtcggcagtc agcagtcggc agtcaggagt
     5221 caggagtcaa cagcccctcc tataaatttt ctaacagata ttgaggctat actgctttac
     5281 cgatcttagg attttttact ttttactttt gagttttgac ttccccgggg ttttcccaaa
     5341 atgagtatat ttagcagggc caccaaagcc ccctgctcat gtccaccagt ctgctgttgc
     5401 ttattatcgt tatctacttt tcacttttat tagtcatttc ccgttttacc tcccggcaaa
     5461 gccgtgataa tacttttttt gatggggatc gtcaatcgcc ctggttcctg gtagcattcg
     5521 ggatgatcgg ttcgggtatt tcggccgtat cactggtgtc gatacccggg aatgtgggca
     5581 ataatcatct ttattatttc cagtttattc tggggagcat tgccggttat ctctttattg
     5641 cttttgtact gctgccgata ttttataaac tacgggtggt atccctttac caatacctgg
     5701 aaagccgttt tggtccggtg agttacaaaa ccggcgcctt gttctttatc gtttcccaat
     5761 cattcggcgc agccctgcgt ctgttattat cagtcaagat cctgcaatat gcgttctttg
     5821 atgaactggg cattccctac gccgtgacta ttgccgtggt acttttatta atctggctat
     5881 ataccaataa atccggggtg aaaaccattg tctggaccga tgccctgcaa tctttattcc
     5941 tgatcaccgt gatcctggta tccatcatca gtatcaaaaa tgcgctaaac ctgagcatag
     6001 gagacatgtt cagctccatt gccagtcatc cctattcaac aattttcgac tgggatccgc
     6061 aatcgggttc caatttcttc aaacaattta tttccggttt actgattacg gtggcactgg
     6121 taggactgga ccagagtatg atgcagaaga ccctgacgat taaagatgcg gcaggtgccc
     6181 ggaaaaatgt actcagtttc agttttttta ttgctgcggc acagaccctg tttctcggat
     6241 taggcgtatt gatgtatcta tatacagaac aaaaggggat acccctgctg aaggaaaatg
     6301 gacagatcct gaataccgat ggcatttatc ccgacctgac ccttcattat ttcgggaaga
     6361 ccggggccat tgcttttttt atcgcggtga tcgcttccac ttttgccagt attgattcct
     6421 gtattacggc gctcacaacg tccttcagtt atgattttat ggattttgat aaaaaatcac
     6481 ctgcagaaag aagacagatc aagaaccggg tattgctggg tgttaatctc gttatgttca
     6541 tcatagtgct ggcattctgg aacagccagg gggccatcat caataccatt tttaaaattg
     6601 ccggatatac ctatggtccg ctcctgggtt tgtacctcac cggtttcttt acttcatata
     6661 agttaaagga gaaatgggtg ccattggctt gtataggcgc tgccttatta acttacgcat
     6721 tgaatgaatt atttactcgt tattaccagt ttgaccttgg ttttatgaat atcgggctga
     6781 atgcaggact gaccatcgta tttttgtatc tgcttaaaag aaaaatgaat gatcaataca
     6841 aggaatatag taatccgtga gtgtcatgac ccgctggtct ttgatatgtg tgcggacctg
     6901 atggcggcta cagacccgtg gattacattg gggatggata aagcccgctg ccgcctcggt
     6961 tttgaagggt tgggcaagga agtacatgta gcatggaacg atgaaatgat tgccggtttt
     7021 acgatcttac aaactgccgg ctctttccgc ggctatattc agacactttg tgccgcacca
     7081 atattcagag gcagcggtgt gggtacccgg ttattgcatt attgcgaaga acgtgtttcg
     7141 gcctattcac cgaatctctt tatttgtgta tcggagttta ataagggggc attgaagctt
     7201 tacaccaact ttggattcaa acaggttggg tcaataccgg attttgtgaa ggaggggtat
     7261 acggagttgc tgctgaggaa gacgagggga ccgttggtaa aaggttgaga ggttgacatg
     7321 ttgaaaggtt gaaaggttga aaagttgaca ggttgacaag ggggtcttct aagaaggttt
     7381 ccttgtcaac atgttaacct gttaactttt caacctacct atcggcaggc aggcttcaat
     7441 acaagctata cgaaagatga agataatacg ctacatacta tttaccctta ccttccccct
     7501 atgggtaaaa gggcaggagc a
//
LOCUS       DAOPMW010000135         7507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11075_length_7507_cov_6.649758, whole genome shotgun sequence.
ACCESSION   DAOPMW010000135 DAOPMW010000000
VERSION     DAOPMW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11075_length_7507_cov_6.649758"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLU11_07830"
     CDS             <1..321
                     /locus_tag="PLU11_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN59065.1"
                     /translation="AKAVMKERLFVITDAVTDTDQGQYPHARSGDKYESGGILSGSAL
                     TMHAAMLNLVRHVQVDPGEAIRMCSHYPARVMGMDDQYGKIQKGYKASLLLLDESLEL
                     IKLL"
     gene            318..1523
                     /locus_tag="PLU11_07835"
     CDS             318..1523
                     /locus_tag="PLU11_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPN59066.1"
                     /translation="MILMHSFKKNGFVILCLLASVQLSAQGFRGQYSPAMKNTYFGVN
                     IGSINYPFSAKQLQPGNTVGSVKVPHTAVRLVLVGHEFNKNISAQITYMRPVKWVEYR
                     NVNNTNQTLTVWMNVAGLTIAGKLPLSQKITLFTEAGLGLIMRRGFNMNNQPVVKSAS
                     YATGLFGANLQYKLNHKWDLQIGTTYSPANDKELQPATVFHSAGFHYTLRELSKERVD
                     LVKKAGYHFPKQILSMAYASNVLGYGANNFVSKGVVPIFWGGEVKARHGLLLNYQHNL
                     FHSRKVFSLDWGIQAGFWKTDRDGTNFFTLSAYPMMRFHTIRSKKTDFYLEYSVAGPS
                     FISKTKMDGLDTGEKFTFYDQMGMGVFTGKDKNLNLGLRIAHFSNGNLFPNNDGVMVP
                     LTFSLGYSF"
     gene            1604..2620
                     /locus_tag="PLU11_07840"
     CDS             1604..2620
                     /locus_tag="PLU11_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPN59067.1"
                     /translation="MKKLLAFATVCICVFAAVYITSCNNSTDTSKETDKVKDSLAKAV
                     ENGRYLANHVAACIHCHSKRDFNKYSGPVVPGTEGGGGGLFDHTILDAIPGTIYSPNI
                     TPDTETGIGSWTDDEILRAITHGINKKGDTLFPIMPYANYNRMAKEDLLNIIAYLRTL
                     KPIKNTVPARQLMIPISMAYPAPALQASVDNNKRPERSDAVKWGEYLITVADCATCHT
                     PFVKGQPDFAQLYGGGNVFNVANFHVGSANITPDSSGIGNWDEDQFLNKFTLYREEKN
                     YNHNPGLKNTTMPLVDYSGMKDSDLKALYAYLRTVKPVTKYVVKYPGIKVNTVKMGEI
                     KVKN"
     gene            complement(2810..3259)
                     /locus_tag="PLU11_07845"
     CDS             complement(2810..3259)
                     /locus_tag="PLU11_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59068.1"
                     /translation="MKNATILLAALFFLYSCGSSDSEAEALSAPEINMWNIDQDSIGN
                     LTKAQVLAPDVDTLSPDVILRYLNNNNPNIKLEYNKLSGDTLFLKIPEATFLTQQMGS
                     SGPEIYIAEVVYNCTEIPGIKFINLDFKEGDHAQPGTYKRESFKLTP"
     gene            complement(3300..4223)
                     /locus_tag="PLU11_07850"
     CDS             complement(3300..4223)
                     /locus_tag="PLU11_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPN59069.1"
                     /translation="MNKLLLVVFALAGICPVSAQLPEVIDGSLYRHESFKSVFVTPRH
                     VDVWLPEGYSPSKKYNVLYMHDGQMLFDANTTWNKLSWDADDVISLLRKENKIKDLIL
                     VGVWNGGDSRHADYFPQKAFELLSAAERDSIYKAVRSNNASVFHQYSIQSDNYLRFLV
                     QELKPFIDSSYSTYTDREHTFIAGSSMGGLISLYAICEYPGIFGGAACLSTHWPGIFV
                     MENNPIPALFFSYMEAHLPDPATHKIYFDYGDATLDAFYPPLQDKADAVMIKKGFTVN
                     NWQTKYFPGENHSEIAWNKRLHFPLTFLLGK"
     gene            complement(4274..5824)
                     /locus_tag="PLU11_07855"
     CDS             complement(4274..5824)
                     /locus_tag="PLU11_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPN59070.1"
                     /translation="MNRTIRYYRLMWTRHDLPAGLTVFLVALPLCLGIANASGAPVYA
                     GLLSGIIGGLIVSLLSGSHLSVSGPAAGLTTIVAGSIISLGGYSVFLLAVVVAGVFQL
                     LLGLLKAGKISNYFPSSVIKGMLAAIGIMLISKQIPMALGLDKPDFWNSGFSQLFSST
                     EFSSNFKNLNQHITRGALIITVVSLAILIIWQQPFAKKLRVIPAPLAVVITGILLHLL
                     FTHGMPGFFLRTTQSVQVPPNVFAEIRFPDFSQLFSNVEIWKAGLLIGGLASLETLLC
                     VEAVDKLDRHNRITPVNRELLAQGVGNIACGLLGAIPMTAVVVRGAANADAGARTKLS
                     SFTHGLFLLLAVMLIPFLLNMIPLASLAAILIMTGYNLAKPKLFRNLWSLGLKQFLPF
                     IITIVVILSTDLLIGVSIGLLISIYFIVQSNFRDEFRITRSKHLDLETYVIKLNSNVT
                     FLNKVKLRDSLDKIPEYSVLYIDGTDCTYIDYDILEIISEYSSKAHDRHIELHLKGIE
                     KVSVTAVH"
     gene            6114..6650
                     /locus_tag="PLU11_07860"
     CDS             6114..6650
                     /locus_tag="PLU11_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN59071.1"
                     /translation="MEEAESIKSILAGHTAEFTHLVKAYETSVFHIVMGFVHQKEDAE
                     DITQEVFVKAFQSLDRFAGRSSFSTWLYRIAIHTSLNELKRRKRRTLWSMAADMLQLP
                     STNKNPEQALTEKTEQDEIRKAIDRLPEKQRMAFILSRYEELPQRDVALIMEISEGAV
                     EQLLLRARDNLKKTLQHP"
     gene            6692..7003
                     /locus_tag="PLU11_07865"
     CDS             6692..7003
                     /locus_tag="PLU11_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59072.1"
                     /translation="MKKENHIENRIEQLIREERNTELNPYLSTRVMAAIAKQQDRKAG
                     LLPVFLKPVLAMASLTIVVWLGIIAGSAYSSGSTEQATVLTNDATAEHFAFYLQTEKE
                     E"
     gene            7005..>7507
                     /locus_tag="PLU11_07870"
     CDS             7005..>7507
                     /locus_tag="PLU11_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59073.1"
                     /translation="MLRKTQLLTWLVVLLLVLNAVTIGTIIYHQRQEKKAASELSIGT
                     NGGTNPLNGRFFRQEMGFNSQQMDVFRELNQSFRPASMEITFRIDSLKEVMYNQLVQV
                     KSDSVQLQQLSDEIGVLHSQLKKETVHFYIRLKEICNPDQQQQLAEVFKPLFISENLM
                     DHGNYRNG"
BASE COUNT         2153 a         1626 c         1802 g         1926 t
ORIGIN      
        1 gccaaagccg tgatgaaaga acgattattt gtgatcacgg acgccgtaac agataccgat
       61 cagggacagt acccgcatgc acgaagtggg gataaatatg aatccggcgg tattctcagc
      121 ggctcggccc tcacgatgca tgcggccatg ctcaacctgg tccgtcatgt acaggtggat
      181 ccgggcgaag cgatccgcat gtgcagtcac taccccgccc gggtaatggg aatggacgat
      241 caatacggaa aaattcaaaa aggatataaa gcgtcgctgc tgttgctgga cgaatcactg
      301 gagctaatca aactgctatg atcttgatgc attcattcaa aaaaaacggg tttgttattt
      361 tatgcctgct ggcgtcggtt cagttatccg cccaggggtt ccgcggccag tattctccag
      421 ccatgaagaa tacatatttc ggagtgaata ttggttctat taattatcca ttttccgcca
      481 aacaattaca gccgggcaat acggtcggat ctgtaaaagt accacacacc gctgtccggc
      541 tggtgctggt gggtcatgag tttaataaga acatttccgc gcagatcact tatatgcgtc
      601 cggtgaaatg ggtcgaatac cgcaatgtca acaataccaa tcagaccctt acggtttgga
      661 tgaatgtggc aggattgaca atagcgggga aactcccatt gtcccaaaaa ataaccttgt
      721 ttacagaggc gggcctgggc ctgatcatgc gtcgcggttt taatatgaat aaccagccag
      781 tggtgaagag tgcctcgtat gccaccggat tatttggcgc caacctccaa tataaactga
      841 atcataaatg ggatttgcag atcggtacca cgtattcgcc ggccaatgat aaagagctgc
      901 agccggcaac ggtctttcat tcagcaggtt tccattacac cttacgggaa ctgagcaagg
      961 aacgggtgga tcttgtaaaa aaagcgggct atcattttcc caaacagatt ctgtcgatgg
     1021 cctatgccag caatgtactc gggtatggcg ccaataattt tgtatccaaa ggtgtggttc
     1081 ccattttctg gggcggggag gtaaaagccc gacatggttt gttgctcaat taccagcaca
     1141 atctgtttca tagccgcaaa gtgttttcgc tggactgggg tatacaagcc ggattctgga
     1201 aaacagaccg ggatggcacc aattttttta ccctgagtgc ttacccgatg atgcggtttc
     1261 atacgatccg ctcaaagaaa acggatttct atcttgaata ctcggtggcc gggccttctt
     1321 ttatatcaaa aacaaaaatg gacggactcg atacgggtga aaagttcact ttttatgatc
     1381 agatggggat gggtgtgttt accggcaagg ataaaaacct gaacctggga ctccggattg
     1441 ctcatttctc caacggaaac ctgtttccca ataatgacgg ggtaatggtg ccgctgacct
     1501 tcagtctcgg gtattctttc tgataataat tttcccggag ttgattttat cagtaccttt
     1561 ttactgcaaa taccattacc atttaacaac aaccaaccac attatgaaaa agctgcttgc
     1621 tttcgcaacg gtatgcatct gcgtattcgc tgcggtttac attacctctt gtaataacag
     1681 caccgacaca tctaaagaaa ccgacaaagt aaaagactca ctcgccaaag cggttgaaaa
     1741 cggtcgctat ctcgccaatc atgtggctgc ctgtatccat tgtcatagta aaagggattt
     1801 caataaatat tcaggtcccg ttgttcccgg aaccgaaggc ggtggtggtg gtttgtttga
     1861 ccacacgata cttgatgcca ttcccggtac tatttatagt ccaaatatta ccccggacac
     1921 ggaaacaggt attggcagct ggaccgatga tgaaatactg cgggcgatta cccatgggat
     1981 caataagaaa ggagatacct tatttcccat tatgccttat gccaactata accggatggc
     2041 caaggaagat ctgctgaata ttattgccta tctgagaacg ttgaaaccga ttaagaatac
     2101 ggtacctgcc cgtcagctga tgattccgat cagcatggcg tatccggcac cggcacttca
     2161 ggcctcggtt gataataata agcggccgga acgttccgac gccgtgaaat ggggagagta
     2221 cctgatcact gtggccgatt gtgccacctg tcatacaccc tttgtaaaag gtcagccgga
     2281 ttttgcccaa ttgtatggag gcggaaatgt atttaatgtg gccaattttc atgtgggttc
     2341 agcgaatatc actccggatt cttctggtat aggtaactgg gatgaggatc agttcctgaa
     2401 caagttcaca ctgtatcgtg aagaaaaaaa ttacaatcat aatccaggcc ttaagaatac
     2461 aacaatgccc ctggtggatt attcaggcat gaaagacagc gacctgaaag cactgtatgc
     2521 ttatttaaga acagtaaaac cggtaacaaa atatgtagta aaatatccgg gtataaaagt
     2581 gaatacggtg aagatggggg agattaaggt gaaaaattag tcaattagcc aatatgccaa
     2641 tgtgccaatg agggaagaag caaatagttt gatagtcgtt tataataggg aggtcaattc
     2701 cctgaaattt aaaagtggta gttttaatgt aaataaattc cagggaaata gcttaactcc
     2761 cttagcaggc caattggcac attggcatat tggttaattg gcacattatt taaggcgtta
     2821 atttaaagct ttctctttta tacgtgccgg gttgcgcatg atcgccttct ttgaagtcga
     2881 ggttgatgaa tttaatgccg ggtatttccg tgcagttata aaccacttcg gctatgtaga
     2941 tttcgggacc ggaagagccc atctgctggg tgaggaaagt ggcttcgggg attttgagaa
     3001 aaagggtatc gccggataat ttattatact ccagttttat gttgggattg ttattgttca
     3061 ggtaacggag gatgacatct ggtgaaagag tatctacatc cggggccaat acctgtgctt
     3121 tcgtcaggtt accgattgaa tcctgatcaa tattccacat attgatttcc ggggcagata
     3181 atgcttcagc ttcactatca gatgagccgc atgaatatag aaagaaaagg gctgctaata
     3241 agattgtcgc gtttttcata tcagtaggtt tataccttaa aggtacaatc catactggct
     3301 tatttaccta atagaaacgt taaagggaaa tgcagtcgtt tattccaggc gatttcacta
     3361 tggttttcac cggggaaata ctttgtctgc cagttgttta ccgtaaaacc tttcttaatc
     3421 atcacggcat ccgctttatc ctgcaagggc gggtaaaagg cgtccagggt cgcatctcca
     3481 tagtcaaaat agatcttatg tgtagcagga tcaggcaggt gagcttccat atagctgaaa
     3541 aaaagggcgg gtatcggatt gttttccatt acaaatattc cgggccagtg agtagacagg
     3601 caggcggccc cgccaaatat cccgggatat tcacatatgg catagaggga aataagtccg
     3661 cccatactgc tgccggcaat aaaagtatgt tccctgtcag tatacgtaga gtaagatgaa
     3721 tcaataaagg gtttcagttc ttgcaccagg aagcggagat aattatctga ctgaatgctg
     3781 tattgatgaa acacagaggc attattactt cttaccgcct tatagatact atcccgttca
     3841 gccgcactca gtaattcaaa tgctttttgc gggaaatagt cggcatgcct ggagtcgcca
     3901 ccattccata ctcccaccag gatcaggtct ttaatcttat tttcttttcg caacaagctg
     3961 attacatcat cggcatccca gcttagtttg ttccaggtgg tattggcatc gaaaagcatc
     4021 tgaccgtcgt gcatataaag cacattgtat tttttggagg gggaatatcc ttcgggcagc
     4081 cagacatcta catgccgtgg agtgacaaat actgacttga aagattcatg acggtacagg
     4141 gatccatcaa ttacttcagg cagctgggca gatacagggc atataccagc caaggcgaat
     4201 acaacaagca agagtttatt cataccatgt gtgcatttat aaaaaacagc cagttcctgt
     4261 aatacctgat ttattagtgc accgccgtta cactcacttt ttcaattccc ttcaggtgca
     4321 gttcaatgtg ccggtcatgc gctttactgg aatattcact gatgatctcc agaatatcat
     4381 aatcgatata ggtgcaatcg gttccatcaa tgtacagtac actgtattca ggaattttat
     4441 cgagggagtc ccggagtttt actttattga gaaaagtgac attgctgttc agtttgatca
     4501 cataggtttc cagatcgagg tgcttcgacc tggtgattct gaattcatcc ctgaaattgc
     4561 tttgtacgat aaagtaaatg gagatcagca gaccgatact cacaccgatc agcagatcgg
     4621 ttgaaagtat caccacaatt gtgatgataa agggcaggaa ttgtttcagt ccgaggctcc
     4681 atagattgcg gaagagtttg ggtttggcca ggttataacc cgtcataatc aggatggcgg
     4741 ccaatgaagc cagcggaatc atattgagca ggaaggggat cagcataacg gccagtaaaa
     4801 ggaacaggcc gtgtgtaaag gaagaaagtt tggtgcgggc accggcatcc gcattggcgg
     4861 ccccacgaac cacgaccgcc gtcatcggaa tagctccgag cagcccgcag gcgatattac
     4921 ccaccccctg tgccagcaat tcgcggttaa ccggtgtaat cctgttgtgg cggtccagtt
     4981 tatctaccgc ttccacacat aaaagggttt ccaggctggc cagtccgccg atcagtaaac
     5041 ccgctttcca gatctccaca ttcgagaata actggctgaa gtcgggaaaa cgtatttcgg
     5101 caaatacgtt tggtggtacc tgtaccgatt gtgtggtgcg caggaaaaaa ccgggcatac
     5161 catgtgtaaa tagtaagtgt agcaaaatac cggtaatgac cacggccagt ggcgcgggaa
     5221 tgacccgtaa ttttttggca aatggttgtt gccagataat aaggatagcc agcgacacta
     5281 cggttataat caacgcaccc cgggtaatat gttgattcag gtttttaaaa ttactgctga
     5341 attcggtgga ggaaaataac tgggagaagc cgctgttcca gaaatcgggt ttatccagcc
     5401 cgagggccat cgggatttgt ttggagatca gcatgatgcc gatagcggcc aacatgccct
     5461 tgatcacgga agaaggaaaa tagttggaga ttttgccggc tttcaataag cccagtaaca
     5521 gctggaacac accagctacc actacggcca gcaggaaaac ggaatagccg ccaagagaaa
     5581 taatggagcc ggctacaata gtagtcagac cagctgccgg acccgaaacg ctgagatggg
     5641 acccgctgag taaggacaca ataagaccgc cgatgatgcc tgaaagtaag cccgcatata
     5701 ccggtgcgcc ggaggcattt gcaataccaa gacaaagcgg gagggctact aagaatacag
     5761 taagaccggc gggcaggtca tgtcttgtcc acatcagccg gtagtacctg atagtgcgat
     5821 tcacagtaaa taaagagttg agtgtagggt aaaggtaaaa aattaccggg ggataaaaga
     5881 tacttatacg tacaaatagc aaattaaccg tagctgcaac agtggacagc cccttttttt
     5941 aataaaagag ccgtttatcc gttgtgcttg ttggcaaatc agtctgccag ctgattaact
     6001 atgtaaatgg ccgtgacctg ggttactgta gaatctgact aaatcgactt ctttacattt
     6061 ccggattgtg tcttcgaatc aatcacctgc cgatccggaa tagtagattc cttatggagg
     6121 aagccgaaag tataaaatcg attctggctg gtcatacggc tgaatttacc catctggtga
     6181 aagcgtatga aacatcggtg tttcacatcg tcatgggatt tgtgcaccag aaagaagatg
     6241 cagaagatat cacccaggag gtttttgtaa aagcctttca gtcattggac cgttttgctg
     6301 gccgttcctc tttttccacc tggctttacc ggatcgccat ccataccagt ttaaatgaac
     6361 tgaaaagaag aaagcgccgg accttatggt ccatggctgc agacatgctg cagctcccct
     6421 ccaccaataa gaatcccgaa caggcattga ctgaaaagac ggaacaggat gaaatccgta
     6481 aagcgattga ccggcttccc gaaaaacaac ggatggcctt tatactcagt cgttatgaag
     6541 aactccccca gcgtgatgtg gccctgataa tggaaataag cgaaggggcg gtggagcagt
     6601 tattacttcg cgcccgggac aatctgaaaa aaactttaca gcacccatag gaaaccgaat
     6661 tcaaccattg tctaattaac caggagcgac tatgaaaaaa gaaaatcaca tagaaaacag
     6721 gatagagcag ctgatccgcg aagagcggaa tacggagctg aatccttatt tgtctacccg
     6781 tgtaatggca gcgattgcga aacagcagga tcgaaaagcc ggcctgttgc cagtgttcct
     6841 gaaacctgta ctggcgatgg ccagccttac aatcgtggtt tggttgggta ttatcgctgg
     6901 cagtgcttat tcatccggaa gtacagaaca ggcaacggta cttaccaatg acgccactgc
     6961 tgagcatttt gctttttacc tgcaaaccga aaaggaggaa taagatgctg cgtaaaacac
     7021 aactattaac ctggctggtg gttttattac tcgtgctgaa tgccgtaacc atcggaacca
     7081 ttatctatca tcaacgccag gagaaaaaag cggcatcgga actcagtatc ggtactaatg
     7141 gtggcaccaa cccgttgaac ggacgttttt tccggcagga aatgggattc aatagtcagc
     7201 agatggatgt tttccgggaa ctgaatcaat cattcagacc tgcttcgatg gaaatcactt
     7261 tccggattga ttccttaaaa gaagtgatgt ataaccagtt ggtccaggta aagtccgata
     7321 gtgttcagct tcaacaatta tccgatgaga tcggggtctt gcacagtcag ctcaaaaagg
     7381 aaaccgttca tttttatata cgtctcaaag aaatatgtaa tcctgatcag caacaacaac
     7441 tggcggaagt tttcaaaccg ctatttataa gtgaaaacct gatggatcat ggaaattacc
     7501 ggaacgg
//
LOCUS       DAOPMW010000136         7487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11127_length_7487_cov_8.974973, whole genome shotgun sequence.
ACCESSION   DAOPMW010000136 DAOPMW010000000
VERSION     DAOPMW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_7487_cov_8.974973"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLU11_07875"
     CDS             <1..324
                     /locus_tag="PLU11_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59074.1"
                     /translation="LTAPDKYKKVKFVNPAYEKQSLKWKACFRAGKEVNDIITIFVKE
                     WLKGDIISKEKIKMPDFKPAETAPPPASGPVASPASTSKTDTIAKPIKEKKSLLNKLK
                     KATGF"
     gene            347..2164
                     /locus_tag="PLU11_07880"
     CDS             347..2164
                     /locus_tag="PLU11_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59075.1"
                     /translation="MKKIILLSAFILSGVFGNTQTEYSDQYLGWIRKINAADPVKPAR
                     VDHRQYSAKQLSTCNLFISWIQASYMPKGGLGEGRRFANEKLSPYNQYTRSLHNYYGA
                     FLPTYVFLKKKAGGGWTPENNLGLFLRVTANGLVGDHVDVISSTEQYYFYIPAVQSND
                     EYARSNQQFMGFNTHPAFSRYIHYYQPKSIRFLSQYNVILSQNNERPWVQITKGEFLE
                     QLEKSINRTHTENLKKINEDYNEKRKQSFLENESALYKRRMDALAGQKEKYRNRLNEK
                     ATIYTEQPSVHLEYTPDLFEGNGSTNDKIPVYKFDPARTANASTDRPQWITIGWGGGE
                     MYDESFKNLHSSMLNNLDIEYIYNYFFDPEKIKGNPYLPLRPPVRKEDPAAKTESANA
                     IKQKAVPGTILFDDFSNTLPGKTPVSWKSGNNYYGEPAKVELHPENGDQWAVIKGQQL
                     EQKEKINLPANFTFTCDIAVPKGYTWGAKRLVIRFGTDKASFLVSMRPGYDGNPGFLY
                     AGPDDFGSSILQQGSTAKASEIPIPGFSNNMPINKYQLQVRKKGQLLELWVNQKQVFS
                     NTNAFTENSITIRGIGFSHSRSDSDYEKYFIDNIKIMNE"
     gene            2185..4083
                     /locus_tag="PLU11_07885"
     CDS             2185..4083
                     /locus_tag="PLU11_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59076.1"
                     /translation="MKYRTILTCIITILFSSALLAQFTPEQRIEDSVIGWWDNLKFDK
                     KLTAENNPDRKRKIDHLDKLVEWMKKSYTPVAGLGTYTRFINTNNYGVQFAVWNVSFN
                     KEWLDNKGKFRPIPEELTKFGVYINSITGSFPIGFINKPGQYVFTWQPDGYSPNEQAA
                     RNRNQLDQGIHANANKFITHVNEVVTVYLAPGNKLPFIPVKLGEYLDMAEASLERQLE
                     NERKDVEAKWPGNLKAQQDAFAYRKNTVEKHRVNIQQLRVRHKNRLNEPAVTREMQPT
                     MYSFQLDPDPFTITAQDNRLKSYFPIYKISPEVLEKCKTSEPQWIAIWVPFETKENGN
                     QLWEMYQSVTENFNYEYVYNHFFSPEKNKGISYFASNKEELAARLDNYRNKGNQGMPA
                     SLQNLPEGLYFKDDFSGNAEGSKPAGWYFSSYGKHSTISTHKKYPGKWLKLGYNSDVT
                     PAFLKKPIPEQFTLEFDLVTDTFSTRTGGAVKLHLSSYPLLEDGREKSGSPGYSLDLI
                     IQAGNEADLTNNNFRGEIRMELHSQPAIYKENYAEGLYFKTATRLLTNKKTKTHIKLD
                     VKNGNISLFMNDQAVATLADFKMSYGKECPGCKLPSGTRFNTLNFRNITNDAENTGLF
                     ISNVRIRL"
     gene            4250..4633
                     /locus_tag="PLU11_07890"
     CDS             4250..4633
                     /locus_tag="PLU11_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59077.1"
                     /translation="MKKTFAIIGSLLIAAGLKAQTIPTVRKETAPPVKKEQTPVSDTA
                     IVKGDKNATDTHIKNTTGTIKETNSRFKKTNTTIKQTGNTLKLKNELQLKEFKETNDT
                     TSKAIKITGAKAMKDVPALKKTNLK"
     gene            4639..5052
                     /locus_tag="PLU11_07895"
     CDS             4639..5052
                     /locus_tag="PLU11_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPN59078.1"
                     /translation="MVFWNSTIPQPAGILAKPSPLHGLGVFAEKSFTINDVIEKAPLI
                     LLKESDKELLEYTRLYHYYFVVDNPKTPAALGLGLSSLYNHSSAANARYRINVKKEYL
                     EIIACKPVTPGEEITLNYNGRPNDPSPVYFPPADV"
     gene            5049..5450
                     /locus_tag="PLU11_07900"
     CDS             5049..5450
                     /locus_tag="PLU11_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPN59079.1"
                     /translation="MSHQLYIKNIKGKGRGVFCKEPILRDEVIEICQLIVLPASDYDT
                     AVSSRLADYFFNFVKEDRTLTLVLGFGSLYNHAIHSNAAYQLDRESRTMIYYALEDIP
                     AGKEININYSGEQGVTYTEWFESRNIQFREK"
     gene            5539..6345
                     /locus_tag="PLU11_07905"
     CDS             5539..6345
                     /locus_tag="PLU11_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPN59080.1"
                     /translation="MYNSRLIVLLCVVLLAGACKRVPVTGRKQLNLVPNSLITTMSFS
                     AYDSVLKISPTLPSTDQRAQEVIKVGTNIRMAVEKYMQQNNLSRELKNFNWEFNTIHE
                     NTINAWCMPGGKVVVYTGLLPVTKNESGLAVVMGHEIAHAIARHGNERMSESLLINLG
                     GLVLEEALKDKKQETQFLFLGLYMVGSNLALSLPNSRMQESEADKLGLIFMSMAGYDP
                     AEAIPFWQRMAALNNSKLPQFLSTHPSDDTRIKKLNELIPEIKATYYNPR"
     gene            complement(6357..6980)
                     /locus_tag="PLU11_07910"
     CDS             complement(6357..6980)
                     /locus_tag="PLU11_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59081.1"
                     /translation="MNKINIVIVDDHPMVLEGMKAMLNQISFVTLNGIASNAFQAIEL
                     LKGCEPDILITDINMPEVSGIELTLKVRKEFPQVKVIAMSTFKERSYISQMIQNGASG
                     YLVKSASKEEIEEAILSVYEGKLYMSLDVNLSASDKQEMTNVPVLSSREKEVLQLIGE
                     GLTNPQIAAKLFISLHTVDSHRKNLLTKFGVNNTAGLIKQAAKHNML"
     gene            complement(6973..>7487)
                     /locus_tag="PLU11_07915"
     CDS             complement(6973..>7487)
                     /locus_tag="PLU11_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59082.1"
                     /translation="LSEEMGRTFNNALNKLDESINEMRRVAHNMMPEALLKLGLQQAL
                     QDYCDGLSESSGGFRITGEFHGLDKRLDASTEVVVYRIVQELLNNAVKHSGADQILAQ
                     VMRQEQALTITVEDNGKGYDIADPDSKSGAGLRNIRSRVDYLKGQLDIQSLPGKGTSI
                     HIDCLIDNHE"
BASE COUNT         2359 a         1741 c         1539 g         1848 t
ORIGIN      
        1 ctaaccgctc cggacaaata caaaaaagtg aagtttgtga accccgccta tgaaaagcaa
       61 tcgttgaaat ggaaagcctg cttcagggct ggaaaagaag taaacgatat cataaccatt
      121 tttgtaaaag agtggctgaa aggggacatc atcagcaaag agaaaatcaa aatgcctgat
      181 tttaagccgg cggaaacagc cccccctcct gcatccggac cagttgcttc gcctgccagc
      241 acttcaaaga ccgatacaat tgccaaaccc ataaaggaaa aaaaatcatt gctgaataaa
      301 ctgaaaaaag ctaccggatt ctgaacactc attctaaaaa cagtatatga aaaagattat
      361 cctgttatct gcctttattc tatccggtgt tttcggaaac acccaaacgg aatacagtga
      421 tcaatacctc ggctggatca gaaaaattaa tgccgctgat cctgtaaaac cggcccgggt
      481 tgatcaccgg cagtattctg ctaaacaact ttccacctgt aacctgttta tcagctggat
      541 acaggccagc tatatgccca aaggcggatt gggtgaagga cgccggtttg ccaatgaaaa
      601 actaagtcct tataatcagt acacccggtc cctccataat tactatggtg cattcctgcc
      661 cacctatgta ttcctgaaga aaaaagccgg tggtggctgg accccggaaa acaaccttgg
      721 acttttcctg cgtgttaccg ccaacgggtt agtgggagat catgtggatg taatcagttc
      781 caccgaacag tactatttct atatacccgc tgttcaatca aatgatgaat atgcccgttc
      841 caaccagcaa ttcatgggat ttaataccca tccggcattc agccggtata ttcattatta
      901 ccagccaaaa agtatccgct ttctctccca atacaatgtg atattgagcc aaaacaatga
      961 acgaccctgg gtgcagatta ctaaaggaga gttccttgaa caacttgaaa aatccatcaa
     1021 ccggactcat accgaaaacc tgaaaaaaat taatgaggat tacaatgaaa aaagaaaaca
     1081 aagtttttta gagaatgaat cagctctgta taaaagaagg atggatgcac tggccggcca
     1141 gaaagaaaaa tacagaaacc ggctgaatga gaaagccacg atctacaccg aacagccctc
     1201 cgtgcatctg gaatacaccc ctgacctgtt tgaagggaac ggaagtacca atgataaaat
     1261 accggtatac aaatttgacc ctgcccgtac cgccaatgcc agtacggaca ggccacaatg
     1321 gatcaccatc ggttggggag gcggtgaaat gtatgatgaa tcatttaaaa acctgcattc
     1381 ttccatgctg aataacctgg atattgaata catctataat tacttttttg acccggaaaa
     1441 aataaaaggt aacccttacc tgccactccg tcctcctgta aggaaagaag acccggcagc
     1501 gaaaactgaa tctgccaatg ccattaaaca gaaagcagta ccgggcacga tcctgtttga
     1561 tgatttttcc aatacactac ccgggaaaac gcctgtcagc tggaaatccg gaaacaatta
     1621 ttatggtgaa ccggcaaagg ttgagttgca tcctgaaaat ggcgatcaat gggctgtcat
     1681 aaaaggacag caactggaac aaaaagagaa aatcaatcta ccggcaaatt ttacatttac
     1741 ctgcgacata gccgttccaa agggatatac ctggggagcc aaacggctgg tgatccgttt
     1801 tggaaccgac aaagcctctt tcctcgtgag tatgcggccc gggtatgatg gaaatcccgg
     1861 attcctgtac gccggacctg acgatttcgg atccagcatt ttgcagcaag gcagcacggc
     1921 caaggcatca gaaattccga ttcccggctt ttccaataat atgcccatta acaaatatca
     1981 gctacaggta cgaaaaaaag ggcaattgct ggaattatgg gtgaatcaaa agcaggtatt
     2041 cagtaatacc aatgctttta ctgaaaatag tataactatc cggggaattg gattttccca
     2101 ttcacgaagt gacagtgatt atgaaaaata ttttattgat aatattaaaa taatgaacga
     2161 ataatcacca tttaaaaaaa ataaatgaaa taccggacaa ttctgacctg tatcatcacc
     2221 attttattct cctccgcctt actggctcaa ttcacccccg aacagcggat tgaagacagc
     2281 gtgattggct ggtgggataa cctgaaattt gacaaaaaac taactgcgga aaacaatccg
     2341 gaccggaaaa gaaaaatcga ccacttggac aaactggtgg aatggatgaa aaaaagttat
     2401 actccggtag ccggacttgg cacgtatacc cggtttataa acaccaataa ctatggggta
     2461 caatttgccg tctggaatgt gagttttaac aaagaatggc tggataacaa aggaaagttc
     2521 aggccaattc cggaggaact gacaaaattc ggtgtttaca tcaattcgat aacaggcagt
     2581 ttccccattg gatttatcaa taaaccgggg caatatgttt ttacctggca accggatggc
     2641 tatagtccca atgaacaagc cgccaggaat agaaaccaat tggatcaggg tattcatgca
     2701 aatgccaata agtttatcac ccatgtcaat gaagtggtaa ccgtctacct ggccccgggt
     2761 aacaaattac cgtttatccc ggttaaactg ggagaatacc tggatatggc cgaagcgtcg
     2821 ttggaacggc agcttgaaaa tgaacgtaaa gacgtggaag ccaaatggcc gggtaacctg
     2881 aaagcacaac aggatgcttt tgcctatcgg aaaaacaccg ttgaaaaaca ccgggtcaac
     2941 attcaacaac tccgtgtccg gcataaaaac aggctgaatg aacccgcagt aacccgggaa
     3001 atgcagccaa ccatgtacag ttttcaactg gatccggatc cgtttaccat caccgcacag
     3061 gacaaccggt taaaatcgta tttcccgata tataaaatca gtccggaagt tcttgaaaaa
     3121 tgtaaaacaa gtgagccgca atggattgcc atatgggtgc cgtttgaaac aaaagaaaac
     3181 ggtaatcaac tgtgggaaat gtatcaaagt gtaactgaaa atttcaatta cgaatacgtt
     3241 tacaatcatt tctttagtcc ggaaaaaaac aaagggattt cctatttcgc atccaataaa
     3301 gaagaattag cggccaggct cgacaactac cggaataaag gaaatcaggg aatgcccgct
     3361 tcgctgcaaa acttaccgga aggcctctat tttaaagatg acttctcggg caatgcagaa
     3421 ggcagcaaac ctgccggatg gtatttctcc agttatggta aacactccac catttccacg
     3481 cacaaaaaat atccgggcaa atggctgaaa ctgggatata actcggacgt cacacctgcc
     3541 tttttgaaga aaccgatacc tgaacaattt acgcttgaat ttgacctggt aaccgatact
     3601 ttttcaaccc gaaccggagg ggcggtaaag ctgcacctca gttcgtatcc tttattggaa
     3661 gatggaaggg aaaaaagcgg atctcccggc tattcgctgg atcttatcat tcaagcaggc
     3721 aatgaagctg atttgactaa caataatttc aggggtgaaa tccgaatgga attgcatagc
     3781 caaccggcta tctacaaaga aaattacgcc gagggattat actttaaaac agctacccgg
     3841 ttactgacga ataaaaaaac caaaacacat attaagctgg atgttaaaaa tggaaatatc
     3901 agcctattta tgaatgacca ggccgtggca actctggcag actttaaaat gagttatggt
     3961 aaagaatgcc ccggatgcaa actgccatcc ggtacccgtt tcaacaccct gaacttccgt
     4021 aatatcacca atgatgcaga aaatacggga ctattcatca gcaatgtccg tatccggctc
     4081 taagcttttc agatacggac ttctcctgga atgaggctgg tggaagacgg gtgtaaccta
     4141 aaaaagcaaa aaatcccact ttcgggggag aacatcattc cggattacaa ttaattttaa
     4201 ttcagatcac aaaatcatac tcccgtaatt caaactaaaa attaatcaaa tgaaaaaaac
     4261 attcgctatc atcggctctt tgctgattgc cgccggtctt aaagcccaga ccatacccac
     4321 ggttagaaaa gaaaccgctc ccccggtaaa aaaagagcaa acacctgtca gtgatacggc
     4381 tattgtcaaa ggcgacaaaa atgccaccga tacccacatt aaaaacacca ccggtactat
     4441 taaagaaaca aacagccggt ttaaaaaaac caatacaaca atcaaacaaa cagggaatac
     4501 gcttaaatta aaaaatgagt tacagttaaa agaatttaaa gagacgaatg acacgacctc
     4561 aaaagcaatc aaaatcaccg gagccaaggc tatgaaagat gtgcccgctt tgaaaaaaac
     4621 taacctgaaa taataagtat ggtgttttgg aattccacca tcccacaacc tgcaggcata
     4681 ttggcaaaac caagcccctt gcatgggttg ggcgtttttg cagaaaaatc attcactata
     4741 aatgatgtca tagaaaaagc ccccctcatt ctgttgaaag aaagtgacaa agaattgctt
     4801 gaatataccc ggttgtatca ttactatttt gtagtagaca atccgaaaac accggcagca
     4861 ctcggacttg gacttagctc actttataac cattcatccg cagcgaatgc ccgttacagg
     4921 attaatgtaa aaaaagaata cctggaaatc atcgcctgta agcctgttac gcccggcgag
     4981 gagatcaccc tcaactataa tggtcgtccc aatgacccat cacctgttta ctttcctccg
     5041 gcagatgtat gagccatcaa ctctacataa aaaatatcaa aggcaaaggc cgtggtgtat
     5101 tctgcaagga acccatcctc cgggatgaag tcatagaaat ctgccagttg atcgtactgc
     5161 cagccagtga ttatgacaca gccgtttctt cccgcctggc ggattacttc tttaattttg
     5221 ttaaagaaga ccggaccctc acacttgtac tgggattcgg ttcattatac aaccatgcca
     5281 tacactccaa tgccgcttac cagctggaca gggaaagccg caccatgatc tactatgcgc
     5341 ttgaggatat tcctgccgga aaagaaataa acatcaacta ttccggtgag cagggagtaa
     5401 cctataccga atggtttgaa tcgagaaata tacaattcag ggaaaaataa aattttccgg
     5461 taatccttct ttttcccctt tttaagggag agatttcttt ccagcgcctg ttacttttac
     5521 agatctaaaa catgctgtat gtacaactcc cgcttaatcg ttctcctttg tgtagtgctc
     5581 ctggctggtg cctgtaaaag ggtccctgtt accggccgca aacaactgaa cctggtcccc
     5641 aacagcctga tcaccaccat gtcattttca gcctatgact ccgtattgaa gatcagtcct
     5701 actcttccgt caaccgatca gcgggcccag gaagtaataa aagtgggtac gaatatccgg
     5761 atggccgttg agaaatatat gcaacaaaac aacctgagcc gggaattaaa aaatttcaat
     5821 tgggaattca ataccattca tgaaaacacc atcaatgcct ggtgtatgcc gggtggaaaa
     5881 gtggtggttt atacgggtct tttaccggtg acaaaaaatg aaagcggact agcggtggtc
     5941 atgggacatg aaattgccca tgcgattgcc cggcacggta atgagcggat gagtgaaagc
     6001 ctgctgatta acctgggagg tcttgtgcta gaggaagcat tgaaagataa aaagcaggaa
     6061 acccagtttt tattcctcgg gctgtatatg gtgggttcga acctggccct ttccttaccc
     6121 aacagccgga tgcaggaatc tgaggccgat aaacttggac tgatctttat gagtatggcc
     6181 ggttatgatc cggctgaagc gatacccttt tggcaaagaa tggctgcgct caataacagt
     6241 aagctcccgc aattcctgtc tactcaccca agcgatgata cccggattaa aaaactaaat
     6301 gaactgattc cggaaattaa agcgacgtat tataatcccc ggtaaacccc agccggttaa
     6361 agcatgttat gctttgccgc ctgtttaatt aaaccggcag tattatttac tccgaattta
     6421 gtgagcaggt ttttccggtg actatccacc gtatgaagac tgataaataa cttggctgca
     6481 atctggggat tggtcagccc ctccccgatc agttgcagca cttctttttc ccggctgctt
     6541 aataccggaa cattggtcat ttcctgttta tccgatgcag aaagatttac atcgaggctc
     6601 atatacaatt taccttcata aacagacagg atcgcttctt caatttcttc tttggaagcg
     6661 ctcttcacca ggtagccgga tgccccgttc tgaatcatct gcgatatata gctacgttcc
     6721 ttgaaagtac tcatggcaat cacttttacc tgtggaaatt ccttccgtac ttttaaggta
     6781 agttcgatac cgctcacttc cggcatatta atatccgtga tcaggatatc aggctcgcac
     6841 cctttcagca attcaatggc ctgaaaggca ttactggcaa taccgtttag tgtgacaaat
     6901 gaaatctgat tcagcatcgc tttcatccct tccagcacca tcggatgatc atccacgatt
     6961 acgatattaa ttttattcat ggttatcaat taaacagtct atatgtattg aagttccttt
     7021 accgggtagt gattgaatat ccagctgtcc tttcaggtaa tccaccctgg aacggatgtt
     7081 tcgtagaccg gcccctgact tcgaatcagg gtctgctata tcatatcctt tcccattatc
     7141 ttccaccgta atcgtgagtg cctgctcctg ccgcatcacc tgtgcaagta tctgatcagc
     7201 cccggaatgt ttgacggcgt tatttaataa ctcctggacg atccggtata ccaccacttc
     7261 ggtcgatgca tccagccgct tatcaaggcc atgaaactcc ccggtgatcc tgaaaccgcc
     7321 tgacgactcc gataaaccat cacaatagtc ctgcaatgcc tgttgcagtc ccagttttag
     7381 taaggcctcc ggcatcatgt tatgtgccac cctgcgcatt tcgttaatcg actcatccag
     7441 tttgttcagg gcgttgttaa atgtccgtcc catctcttcc gataaaa
//
LOCUS       DAOPMW010000137         7461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11186_length_7461_cov_5.734810, whole genome shotgun sequence.
ACCESSION   DAOPMW010000137 DAOPMW010000000
VERSION     DAOPMW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11186_length_7461_cov_5.734810"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLU11_07920"
     CDS             <1..737
                     /locus_tag="PLU11_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPN59083.1"
                     /translation="VDEAIEVANKVGYPVLVRPSYVLGGQRMRIVINDDEVEKAVVSL
                     LKHIPGNKILVDHFLDRCQEAEIDAICDGEKFHVMGIMEHIEPAGIHSGDSNAVLPYF
                     NLTPLEVQDMVDYAKKIALKLNVIGLLNIQFAIKEGKVYVIEANPRASRTTPFIAKAY
                     NKPYLNYATKVMLGEMKVQDIPEEKHLDGFAIKEPVFSFNKFPGVNKELGPEMKSTGE
                     AIRFIKNLRDPYFRQLYKDRSMYLSK"
     gene            1045..2592
                     /locus_tag="PLU11_07925"
     CDS             1045..2592
                     /locus_tag="PLU11_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59084.1"
                     /translation="MRAPSAFIWQLIQSLSRSERLYFKRNFIPATGTGDRLYIRLFDA
                     IAAQKVYNEKALLEKFSPSLNKKNIASQKNYLQKQVGDALVQYESIRDPGKDVYASIQ
                     LIRIYRKKGLYDEAFSIWKKAIELARSGEAFAMSNLLKTEFEKMVIFSPSHSDQDELL
                     GIFKKNIITYTEYAELITLRDIYTETLLLKKKTHFGQDSHLLDTIHTLLDRVNQTGKA
                     HQGHSFWYRHYYLMSKATLLYLLNDIAGSLQLLKEHFSNWKKEEIFTSTHGEFYIELL
                     YMINYAGILHGDFDYVTAVFNDPLNKQLTDPYHRAYFEVTRYLALNKIYNKTARYEEV
                     KKLLEVMKANYTSWEPSLNEELNSTLSFSMGVGCFALDQYEDALFFTKRAITTFPEGS
                     RDEHNAVGQLLLLLITFCLNNARLFDAQYRATYQFFYKRKKKQLFEAALKHCLQRCFY
                     MTEQKKKIQEFKKALQLLEENRDNPIQQRAFAIFNFEGWLQSRVMRITYRQYVEKKVR
                     AELKEAV"
     gene            2602..3069
                     /locus_tag="PLU11_07930"
     CDS             2602..3069
                     /locus_tag="PLU11_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59085.1"
                     /translation="MKKAVNDIIQRLENTVNGEPWFGRSALSLLKETNPEFVFMKTGN
                     GGHSLADLLWHMITWAQFTLNRLEKKEEDMEVFESLDWRSIDPAIHSWKNGIKEFTSV
                     FGKIVALLKTKEDAYLEEKVDYRDYNVQFLLNGLIEHTIYHLGQIAYAQKQLS"
     gene            3123..5222
                     /locus_tag="PLU11_07935"
     CDS             3123..5222
                     /locus_tag="PLU11_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPN59086.1"
                     /translation="MALYGSNPLKRSPILQILIPFIIGILYHYYLTLPQWLVIILIPL
                     SLAGLLIFHRIPILARFRHSMLSGIFTAILFFSLGAALSRNQDIRQQSNWFGHHLMDT
                     SSLLLVLEEPLVEKSKSFKAIAAVRASLNNNQAEAVHGKLLIYFSKDSTVRSLGYGSR
                     LIIRQPVREIKSSGNPGAFDFKRYSLFQGITHQVYLRPDDYEVLPGKEQNWFRKQLFA
                     SGQFLVQLLRRYIPGRLESGIAEALLIGYKQDLDPEIVQSYSNTGVVHIIAISGLHLG
                     LIYWLLLLLLKPLNKSKKTRWLHAVLVIAGLWSFSFLAGAQPSVLRSALMFSCMVLGN
                     SVSRKSPAINSLAVSAFILLCYNPYWLWDAGFQLSYAAVLSILLFNQPVYNWFYIKNK
                     LLDIAWKMNAVTIAAQILTLPVSLYLFHQFPVSFLFSNFIAVPLSSLLLLGEIFLCII
                     AFYSPLAEFTGKAIAAGIRLMNAWIERVEQLPFSVWEGIQLSSTQAIWLTGLLLTASY
                     WLMEKSKKAFGVSLFFLLIFSAERFISFREAGGQQKIIVYYVPRMQAIDFIQRRMYWF
                     SGDSTLMQNQLSRNFHLKPSRVLHRVSETDRLNGLLHYEYYYRFGGKNIICLDTTIAY
                     SAPADSIRPVIDLLVLSGNPKIYLKRLAAVLDIRQLVIDGSVPARKAGFWKKDADSLQ
                     IPFHDTGEKGAFVMSLN"
     gene            5271..6782
                     /gene="purH"
                     /locus_tag="PLU11_07940"
     CDS             5271..6782
                     /gene="purH"
                     /locus_tag="PLU11_07940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPN59087.1"
                     /translation="MDKKIQSALISVFYKDGLENIVTELDRLGITIYSTGGTQSFIEK
                     LGIKVVPVEDLTSYPSILGGRVKTLHPSVFGGILGKRDDETHLAEMKQYNIPEIDLVI
                     VDLYPFEETVASTSEEKAIIEKIDIGGPSMIRGAAKNFRDVVVIAAKKDYAALENLLK
                     TQHGNTTIEQRKAFAAKAFEVVAHYDVAISKYFTTTDNLYFLESIPEPKIMRYGENPH
                     QKGVFYGDLSQLFDQLNGKELSYNNIVDTDAAMQLIREFTDTTFAIIKHTNVCGIAQR
                     PTVKESWDAALAGDPESAFGGVLVCNGTIDKATAEAINEIFFEVLVAPSYNEDALTVL
                     KSKKNRILLQLKTQPASQVQYKSMLNGVLVQDIDTGNFTDWTETTKRKETPEERADLL
                     FANIVCKHLKSNAIALVKNKQLIGKGCGQTSRIDSLRQALEKAKQFNFDLQGAVMSSD
                     AFFPFDDCVRLGHEAGITAFIQPGGSIRDNDSIDYCSKHNLSMIMTGLRHFKH"
     gene            6861..>7461
                     /locus_tag="PLU11_07945"
     CDS             6861..>7461
                     /locus_tag="PLU11_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPN59088.1"
                     /translation="MNIFDFHKTIVQSSADSLKKLSVFNPFTIHKKGTRTKALFALAA
                     VCFLWGTTWIASKEGVRHMPALQLAGIRQLIAGLIYVAFFLYKGAPLPKGREWIPVLV
                     LCFLNFIMSNGLSTWGVKYISAGLGAIMGAIFPLWIVIIGLFSSSEKIPTKAIIGLLL
                     GFAGVCVIFFDHMMDFMNPEFRLGIILSLIATWTWAFATI"
BASE COUNT         2061 a         1752 c         1683 g         1965 t
ORIGIN      
        1 atgtggatga agccattgaa gtagccaata aagtaggcta cccggtactg gttcgccctt
       61 cctatgttct cggcggacaa cggatgcgga ttgtgatcaa tgatgatgaa gtcgaaaaag
      121 cagtggtgag tttgctgaaa catattcccg gaaataaaat cctggtggat catttcctgg
      181 atcgttgcca ggaagccgaa atcgatgcca tctgtgatgg agaaaaattc catgtaatgg
      241 gcattatgga acatatcgaa ccagccggta tccacagtgg tgacagtaat gcggtactgc
      301 cttactttaa cctcaccccg ctggaagtac aggacatggt ggactatgcc aagaagatcg
      361 ccctgaagct gaatgtaatt ggattgctca atatccagtt tgccatcaag gaagggaaag
      421 tatatgtgat tgaagccaat ccgagagctt cacgtaccac gccttttatt gccaaagcat
      481 ataacaagcc ttacctgaac tatgccacca aggtaatgct gggtgaaatg aaagtgcagg
      541 atattccgga agaaaaacac ctggacggct ttgccatcaa agaaccggtg ttcagcttta
      601 ataagtttcc cggggtgaat aaagaactcg gtcctgagat gaaatcaacc ggtgaagcaa
      661 tccgctttat taaaaacctg cgtgatcctt atttccgcca gttgtataaa gacagaagca
      721 tgtacctgag taaataaaaa aaagctgtac ctgattagtg gtacagctgg ttaatgggta
      781 atggcctctg ttggaacccc cgtggttgga cgggtataaa taaaataaag aagatggctt
      841 gtattatcaa tgccctacaa atctactgcc cggcgtttgc cgggcatttt ttttattcgc
      901 tgaatatctg gttcattata tactatttta ataaaattaa atttgtataa tattgattat
      961 taataattta aacaatattt attttttgat atatttgatt tataaaagat agtttggtat
     1021 ttttcgaaca cagtaatcta atcaatgaga gccccctccg cttttatctg gcagctaata
     1081 caatccctga gcaggagtga acggctctat tttaaacgga attttattcc agccacagga
     1141 accggggacc ggctttatat ccggctgttt gatgctattg ccgcgcagaa agtatacaat
     1201 gaaaaggcct tgctggaaaa attcagtcct tctctgaaca agaaaaatat cgcctcacag
     1261 aaaaactacc tgcagaaaca ggtgggagac gcattggtac aatatgagtc aatccgggat
     1321 ccgggcaaag atgtatatgc aagtatccag ctgattcgta tctaccggaa aaaaggactc
     1381 tatgatgaag ccttctcgat ctggaaaaaa gcaattgagc tggcacggag cggggaagcc
     1441 tttgccatgt cgaacctgct gaaaactgaa tttgaaaaaa tggtcatctt cagtccatcc
     1501 cattccgacc aggatgaatt actgggtatt ttcaagaaaa acataatcac ctatacagaa
     1561 tatgcggagc tgatcacttt gcgggatatt tatacagaga ccttactgct gaaaaagaaa
     1621 acccatttcg gccaggacag tcacctgctg gacaccattc atactttact ggatagagtg
     1681 aaccaaacag gcaaggccca tcagggacat tcattctggt accggcatta ttacctgatg
     1741 agtaaagcga ccctgctcta cctgctcaat gatattgcgg gctcccttca gctcctgaaa
     1801 gaacattttt ccaactggaa aaaagaggaa atatttacat ccacccatgg agagttctac
     1861 atcgaattac tctatatgat caattatgcg ggcattcttc atggcgattt tgattatgtc
     1921 acggccgtat tcaatgatcc gctgaataaa caactgaccg acccctatca ccgggcttat
     1981 tttgaagtga cccgctacct cgccctgaat aaaatatata ataaaacggc ccgctatgaa
     2041 gaagtaaaaa aactgctgga ggtgatgaaa gcaaattata ccagctggga accctccctg
     2101 aatgaagaac tcaacagcac cctgagtttt tccatgggag tgggttgttt tgccctggat
     2161 caatatgaag acgccctttt ctttaccaag cgggccatta ccactttccc ggaaggttcc
     2221 agggatgaac acaatgccgt aggacagtta ttgctgctgc tgattacctt ttgccttaac
     2281 aatgcccgcc tgtttgatgc ccagtaccgg gccacttacc agtttttcta taaacgaaaa
     2341 aagaaacagc tatttgaagc cgccctgaaa cattgcctgc aacggtgttt ctatatgacc
     2401 gaacaaaaaa agaaaataca ggaatttaaa aaggccctgc aactcctgga ggaaaacagg
     2461 gataacccca tccagcaacg tgcttttgcc atcttcaatt ttgaaggctg gctgcaaagc
     2521 agggtaatgc gaatcaccta ccgccagtac gtggagaaaa aagtccgtgc agaactgaaa
     2581 gaggctgttt aaatttaggg tatgaaaaaa gcggttaatg acattattca acgcctggag
     2641 aacaccgtaa atggcgaacc ctggtttggc cgttcggccc tttcactgct gaaagaaacc
     2701 aatccggaat ttgttttcat gaaaacaggt aacggcggac actccctggc cgatctgctc
     2761 tggcatatga ttacctgggc acagtttacc ctgaaccgac tggagaaaaa ggaagaagat
     2821 atggaagttt ttgaatccct cgactggcgt tcgattgatc ctgctattca cagctggaaa
     2881 aacggtataa aagaatttac atcggtcttt ggaaaaatcg tcgccctgtt aaaaacaaaa
     2941 gaagatgctt acctggaaga aaaggtcgac taccgggact acaatgtaca attcctgctc
     3001 aacggtctca ttgaacatac catttaccac ctgggacaaa tcgcctatgc tcagaaacag
     3061 ctaagctgag catacccgca aagtagtatt gtctgccaac gggagcctga gtaatttgag
     3121 ccatggctct ctatggcagc aaccccctga agcgcagtcc catcctgcaa atcctgattc
     3181 cttttattat tggtatcctt taccactatt acctgacgct gccccaatgg ttagtaatca
     3241 tcctgattcc actttcactg gccggactcc tgatcttcca tcgtataccg atattggccc
     3301 gtttccgaca ctccatgctg tccggtattt tcacggctat actcttcttt tcattgggag
     3361 cggccttaag ccggaaccag gatatccggc agcaatccaa ctggtttggt caccacctca
     3421 tggataccag ttcactgttg ttggtactgg aagagccact ggtggaaaaa tcaaaatcat
     3481 ttaaagcgat tgctgccgtc agggccagtt taaataataa tcaggcggaa gcggtccatg
     3541 gcaaactgct tatatatttc agcaaagaca gtacggtgcg gtcactcggt tatggctccc
     3601 ggctgatcat ccgtcaaccg gttcgtgaaa taaaaagttc aggaaatccc ggagcttttg
     3661 attttaaacg atactccctt ttccagggca tcactcacca ggtttatctc cggccagatg
     3721 attatgaagt attgccagga aaggaacaaa actggttcag gaaacaactc tttgcgtccg
     3781 gacaattcct ggtacagctg ctgcgccgct atatccccgg gcgcctggaa tcaggaatcg
     3841 ctgaagccct gctgatcggg tataaacagg accttgatcc ggaaatcgta cagtcctaca
     3901 gcaatacggg agtggtgcat atcattgcga tatcaggact ccacctggga ctgatctact
     3961 ggctgctcct gctgttactg aagccattga ataaatcaaa aaagacacgc tggctgcatg
     4021 ccgtgctggt cattgccgga ctatggtcct tcagtttcct ggccggtgca cagccctccg
     4081 ttctccgttc ggccctgatg ttcagctgta tggtcctggg taattcggtc agccgcaaat
     4141 caccggccat caattcactg gccgtatctg cgtttatact tctctgttat aatccttact
     4201 ggttatggga cgccggtttc caattgtcct atgccgccgt attgagcatc ctgttgttta
     4261 atcaacccgt ttacaactgg ttttatataa aaaacaaatt actcgacatt gcctggaaaa
     4321 tgaatgccgt gaccatcgcg gcacaaatac tcacgttgcc ggtaagtctt tatctgtttc
     4381 accagtttcc ggtctccttc ctgttcagca attttatcgc tgtgcccctg tcgagcctcc
     4441 tcctgctggg cgagatcttt ttatgtatta tcgcctttta ctccccactg gctgagttta
     4501 ccggcaaagc tatcgccgca ggtatccgac tcatgaatgc ctggattgaa cgggtggaac
     4561 aactgccatt ctcggtttgg gaaggtatcc agctgagttc gactcaggcg atctggctga
     4621 ccggtttatt attaactgcc agctactggc tgatggaaaa atctaaaaaa gcctttggtg
     4681 tatccctgtt tttcctgctt atattttcag cagagcggtt tatttctttc cgggaagccg
     4741 gcggacaaca gaaaatcatt gtttactatg taccccgtat gcaagccatt gattttattc
     4801 aaagacgtat gtattggttt tctggcgaca gcactctgat gcaaaaccag ttgtcccgca
     4861 attttcacct gaaaccttcg agggtactcc accgggtcag tgaaacagac cggttgaatg
     4921 ggttgctgca ctatgagtac tattatcggt ttggcggaaa aaatatcatt tgcctggata
     4981 ccaccattgc ctattcagca cctgctgaca gcattcgacc cgtcatcgac ctgctggtat
     5041 tatccggtaa ccccaaaatt tacctgaaac ggctcgctgc tgtactggat atccgacaac
     5101 tggtgattga cgggagtgtg cccgcccgaa aagccggttt ttggaaaaaa gatgcggatt
     5161 cgctgcaaat cccgttccat gatacggggg agaaaggcgc ttttgtgatg agcctgaatt
     5221 aagttaattt gcggcctctt tcgaacgcct gccttcaatt atcatctact atggacaaaa
     5281 agattcaatc agcgttaatt tctgtttttt acaaagacgg actggaaaat attgtaaccg
     5341 aacttgaccg gttgggaata accatctatt ccaccggtgg tacccagagt tttatcgaaa
     5401 agctggggat taaagtggtg cctgtggagg acctgaccag ctatccttcc atcctggggg
     5461 gacgggtaaa gaccttgcat ccttccgttt ttggaggtat tctgggtaaa agagatgatg
     5521 aaacgcatct ggccgagatg aagcagtaca atatccctga aatcgacctg gtgatcgtgg
     5581 atctttatcc ctttgaggaa accgtcgcca gtaccagtga ggaaaaagca atcattgaaa
     5641 aaattgatat cggcggacct tccatgatcc gcggggcggc caaaaacttc cgcgatgtgg
     5701 tggtgattgc ggctaagaaa gactatgcgg cccttgaaaa cctgctgaaa acccagcacg
     5761 ggaatacaac gatcgaacag cgtaaagctt ttgcggcaaa agcttttgaa gtcgtagccc
     5821 actatgatgt ggccatttcc aaatatttta caacaacgga taacctgtat tttctggaat
     5881 ccattccgga accgaaaatc atgcggtatg gagagaaccc acaccagaaa ggggttttct
     5941 atggcgactt aagccagctc tttgatcagc tcaatggaaa agaactctcc tacaataata
     6001 ttgtggatac cgatgcggcc atgcaactaa tccgggaatt cacggatacc acttttgcaa
     6061 tcatcaagca taccaatgtt tgcggcattg cccaacgccc aactgtaaag gaaagctggg
     6121 atgcggccct ggccggcgat ccggaaagtg cttttggcgg cgtgctggtc tgcaatggca
     6181 ccattgacaa agccacggca gaagccatca atgaaatttt ctttgaagta ctggtagccc
     6241 cttcatacaa tgaagacgca ctgacggtac tgaaatccaa aaagaaccgg atcctgttgc
     6301 agttaaaaac acagccagcc agccaggtgc agtataaatc catgctcaac ggggtgttgg
     6361 tacaggatat tgataccggt aatttcaccg actggaccga aaccaccaaa cgtaaagaga
     6421 cacccgaaga aagagcagat ctcctgtttg ccaatattgt ctgcaagcat ttaaaatcaa
     6481 atgccattgc cctggtgaag aataaacaac tcatcggcaa aggctgcgga caaaccagcc
     6541 ggattgattc gctccgccag gcattggaaa aagcaaagca gttcaatttt gacctgcaag
     6601 gtgccgtgat gtccagtgat gcattcttcc cctttgatga ctgtgtacga ctgggacatg
     6661 aagccggtat taccgccttt atccagccgg gcggttccat ccgggataat gattcgattg
     6721 actattgcag caagcataac ctgtcgatga tcatgacagg gctgcggcat tttaaacatt
     6781 gataatacca acgtacagcc tgatctcctg tgcttttttg tatcccgaac aataattcac
     6841 cccacgggga agtgaaaggc atgaacattt ttgacttcca taaaaccatc gttcaatcct
     6901 cagccgattc gctgaagaag ctttcggtat tcaatccttt taccattcac aaaaaaggaa
     6961 cccgtaccaa ggcgctcttt gccctcgccg cggtttgttt tctctggggt accacctgga
     7021 tcgcgtccaa ggaaggcgta cggcatatgc cagccttaca actggccggt atccggcaat
     7081 taatcgccgg actgatctat gtcgccttct tcctatacaa aggtgctcca cttcccaaag
     7141 gccgtgaatg gattccggta ctggtattgt gcttcctgaa ttttattatg agcaatggcc
     7201 tcagtacctg gggcgttaaa tatatttcgg ccggactggg tgccattatg ggtgctatct
     7261 tcccattatg gatcgtgatc atcggacttt tcagttcgag tgaaaagatc ccgacaaaag
     7321 ccatcattgg tttattactg ggctttgccg gtgtttgtgt gatctttttc gatcacatga
     7381 tggatttcat gaacccggaa ttccgcctgg gtatcatctt atcgctgatt gccacctgga
     7441 cctgggcctt tgccaccatt t
//
LOCUS       DAOPMW010000138         7404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11298_length_7404_cov_9.688529, whole genome shotgun sequence.
ACCESSION   DAOPMW010000138 DAOPMW010000000
VERSION     DAOPMW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_7404_cov_9.688529"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..410)
                     /locus_tag="PLU11_07950"
     CDS             complement(135..410)
                     /locus_tag="PLU11_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59089.1"
                     /translation="MKRRVRKIANFFAFNLLFFALYLNFIHRDKAIAATGTTVNASAE
                     AFSGTTIVDNTNPVQEKKTEPSVAVVGKTQKETAKSTDNSALRLSIN"
     gene            complement(558..2597)
                     /gene="uvrB"
                     /locus_tag="PLU11_07955"
     CDS             complement(558..2597)
                     /gene="uvrB"
                     /locus_tag="PLU11_07955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPN59090.1"
                     /translation="MAFQLHTPFQPAGDQPDAIRQLTEGINDGEKFQTLLGVTGSGKT
                     FTIANLIQNTQRPTLILTHNKTLVAQLYGEFRQFFPENAVEYFVSYYDYYQPEAYLPV
                     SDVYIEKDLSINDELDKLRLRATSSLLSGRRDIIVVASVSCIYGMGNPTDYENGIIRI
                     SKGETISRQAFLHSLVNSLYNRTTIDFNRGTFRVKGDTVDINLPYVDYGYRITFFGDE
                     IEEIESIDVVTSKRIAKMENAAIFPANLYVAPKDILQQVLYEIQDEVAAQAEYFKREG
                     KYIEAQRLTERVNYDVEMIRELGYCNGIENYSRFFDRRRPGTRPFCLLDYFPKDFLMI
                     IDESHQTIPQVSGMYGGDRSRKLILVDYGFRLPSALDNRPLNFHEFESMTNQVVFVSA
                     TPDDYELEKCGGVVVEQVVRPTGLLDPPIEIRPSVNQIDDLLEEVDKRIKMGDRVLVT
                     TLTKRMAEEMDKYLHKINIKSKYIHSEVDTLERVEILRELRLGKIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNEKSLTQTAGRAARNVNGLVIFYADKMTDSMRKTIDET
                     IRRREKQVSYNIEHGITPRTIIKSTKDVFAQTSVLDIKGYDPADPYAIAPDGELVTVA
                     AEEQAEYKTIPQLEKALNKVKKEMEKAARDLDFMEAARLRDEMFLMQKKLEEMKK"
     gene            complement(2646..3098)
                     /locus_tag="PLU11_07960"
     CDS             complement(2646..3098)
                     /locus_tag="PLU11_07960"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPN59091.1"
                     /translation="MERRILKECKNIALVAHDHKKKELVEWAVYNKQMLKSHTLHATG
                     TTGKLLEKALGQPVHCFLSGPLGGDQQIGAAIAEGKIDILIFFWDPMEAQPHDPDIKA
                     LLRVAATWNIPVACDRSTADFMLTSPLMHAEYETLLPDYSGYTQRKIK"
     gene            complement(3328..3873)
                     /locus_tag="PLU11_07965"
     CDS             complement(3328..3873)
                     /locus_tag="PLU11_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPN59092.1"
                     /translation="MKKVFLIISLVTLSTAVFAQVKKTSSATISFDATTPKDALPKAD
                     NKTVVGEVNTKNGQVGFEAIVKNFAFSNAMIQDHFNGEKWLNSAKFPTFTFVGKITDL
                     SKVKFDKAGTYSVPVTGELTVRDVTKPLTTTVTFEVKDGSLKATTSFSIKLADYGITG
                     APIEAGKIATEPAITVSAELK"
     gene            complement(3906..4796)
                     /locus_tag="PLU11_07970"
     CDS             complement(3906..4796)
                     /locus_tag="PLU11_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59093.1"
                     /translation="MPQTTIHKNSLHLLSASAMLVSFFLPWVKWEEYKMTGLDLPTGH
                     FFATSAEKFGLANPYPQFNFTFYLFILIPVLTIVASFLHIKGKKAGWIAAIAGSLSLT
                     LVTMYAIFTHTLLDLGVGKSLSGMIQPSLYIHGLAALLFIWTSRPDGILLKTAWILIG
                     PILAYGGYSIVKYQVENETFSNTTKEKPAYTVNAAELIQEFISSDSSANKKYTDKIIV
                     VNGRISELEAADSTINVKFTDSTSGSYAIFDFQAADVAAVKKMAVGDSVSIKASCSGG
                     IFSRLRQATVINFKRSAINK"
     gene            complement(5051..6082)
                     /gene="recA"
                     /locus_tag="PLU11_07975"
     CDS             complement(5051..6082)
                     /gene="recA"
                     /locus_tag="PLU11_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPN59094.1"
                     /translation="MAKQEKSTDMSANSEKLKALKLTMDKIEKDYGKGSVMMMNERAV
                     TEMEVISTGSIGLDVALGIGGLPRGRVVEIYGPESSGKTTIATHVIAEAQKKGGMCAF
                     IDAEHAFDSNYAKKLGVDVDNLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALV
                     PKSELEGEMGDSKMGLHARLMSQALRKLTATISKTNTICIFINQLREKIGVMFGNPET
                     TTGGNALKFYASVRLDIRRTQQVKDGDEAIGNHVKVKVVKNKVAPPFRAAEFDIIFGE
                     GISKNGEIIDMGTELGIVQKSGSWYSYNSDKLGQGRDSVKQLLVDNPELAKEIEGKIR
                     EKIKEMQTT"
     gene            complement(6347..7024)
                     /locus_tag="PLU11_07980"
     CDS             complement(6347..7024)
                     /locus_tag="PLU11_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59095.1"
                     /translation="MNTVLTSASSDHINNQEDQPLALRLMARFISWAFHPVFVPVYVM
                     AFLIYEHPFQFTGFDPRQKMLVMLQAVAMYLFFPLVTVLLLKALGFIESIQLKKQKDR
                     IIPLVACGVWYFWIWYVWHNLPDYPDMAIRFALAIWISSWTSLMLNTRMKISLHAISM
                     GIAVAFLFLLAFTQALNFGLYLALVVFIAGLVCTARFLVSDHTRAEVYGGFALGVLSM
                     LAAQVFG"
     gene            7137..>7404
                     /locus_tag="PLU11_07985"
     CDS             7137..>7404
                     /locus_tag="PLU11_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59096.1"
                     /translation="MKKLLLAFLIITGTFQGFSQKIDKAKMQAMYDSIKAAGIRHPDF
                     VMAQCMQETGNLKCKQCCLRYHNLFGFYIKGNKCKKFESDAACIA"
BASE COUNT         1939 a         1642 c         1724 g         2099 t
ORIGIN      
        1 gaaggctgga gtatgtatca aaagttctca aaagatgtgt ccacaccgta gtcctgaagg
       61 agagggggtg gggggtgagg tataaaaaac cccggctatt gccagggttt ataataaaag
      121 ttaatggggt aaacttagtt gatcgacaaa cgcagggcgc tgttatcagt tgatttagcg
      181 gtttcttttt gtgtctttcc tactaccgct acggaaggct cggtcttctt ttcctgaaca
      241 gggtttgtat tatctacaat ggttgtgccg ctgaatgctt cagcagaggc attcaccgtt
      301 gtaccggtag ccgcaatggc tttatccctg tgaataaaat tcaggtaaag ggcgaaaaac
      361 agcagattga aggcaaaaaa gttggcgatt ttacgaacgc gtcttttcat aacggatgta
      421 ttggatttta aataaaattg gtttctcgga taacagtgct aagataggcc gggtcgttgc
      481 ctcgtacaaa gctttggtgc aggtatttaa tcaaatcggg cagtaatacg ctgaggtaaa
      541 tcgtaaatct acgaggttta cttcttcatt tcttccagct tcttctgcat taagaacatt
      601 tcatctctca gacgggcggc ttccatgaaa tccaggtccc gcgcggcttt ctccatttct
      661 tttttgactt tattcagtgc tttttccagt tgcgggatgg ttttatattc tgcctgttct
      721 tctgcagcca cggttacgag ttctccatca ggagcaatgg catagggatc cgccggatca
      781 taacctttaa tatccaatac agaggtctgg gcaaacacat ccttggtaga tttgattatc
      841 gttcttggag taataccatg ttctatatta tatgatacct gtttttcacg gcgccggatg
      901 gtttcatcaa tggtcttacg catgctatcc gtcatcttat cggcatagaa gattaccagg
      961 ccgtttacat tccttgcagc acgacctgcc gtctgggtaa gtgatttttc attgcgcaga
     1021 aaaccttctt tgtctgcatc gagaatagct accagcgaca cctccggaag atccagtccc
     1081 tcccttaata ggtttacacc caccagtacg tcaatttttc caagtcgcag ttcccgtaag
     1141 atctccaccc gttccagagt atccacttca ctatgtatgt atttggattt gatattaatc
     1201 ttatgcaggt atttatccat ttcctccgcc atgcgcttgg tcagcgtggt taccagtacc
     1261 cggtctccca ttttgatccg cttatccact tcttccagca aatcatcgat ctggtttaca
     1321 ctgggccgga tttctatcgg aggatccagt agcccggtcg gacgaactac ttgttctacc
     1381 accaccccgc cgcatttttc cagttcataa tcatccgggg tagccgacac aaagaccacc
     1441 tggttagtca tggattcaaa ttcatgaaaa ttcagcggac ggttatcaag agcggaaggc
     1501 agccggaatc cgtaatcaac gaggatcagt ttgcggctgc ggtcaccccc gtacatacca
     1561 ctgacctggg ggatggtctg gtgactttca tcgatgatca tcaggaaatc cttggggaaa
     1621 taatccagca gacaaaaagg ccttgtaccc gggcgacggc ggtcaaagaa ccgggaatag
     1681 ttttcgatac cgttgcaata gcccaactcc cggatcattt ccacatcata attcactctt
     1741 tcggtgaggc gttgtgcttc gatgtatttg ccttcgcgtt tgaaatattc agcctgtgcc
     1801 gctacttcat cctgtatttc atataatacc tgttgcagga tatcttttgg cgccacatac
     1861 aaattggcgg ggaagatggc cgcgttttcc attttggcaa tccgcttgga agtcacgaca
     1921 tcgatgcttt caatttcttc aatctcgtcg ccgaagaaag tgatgcggta tccgtaatcc
     1981 acatagggga gattgatatc aaccgtatct ccttttacgc gaaaggtccc ccggttaaaa
     2041 tcaatggtgg tccggttata gagtgaattt acgagggaat gaaggaaagc ctgtcttgaa
     2101 atggtttctc ccttactgat ccggatgatt ccgttctcat aatccgtcgg gttacccata
     2161 ccataaatgc aactcacgga agctaccaca ataatatcgc gtcttccact gagcagactg
     2221 gaggtggccc gcaaccggag tttgtccagt tcatcattta tactcaggtc tttttcaata
     2281 tatacatcac tgaccgggag ataggcttca ggctggtagt aatcgtaata ggatacaaaa
     2341 tactctacag cattttccgg gaaaaactgc ctgaactctc catataactg ggccaccagg
     2401 gttttgttat gggtgagtat cagggtgggc cgttgggtgt tctggatcag attggcaatg
     2461 gtaaaagttt taccgctgcc ggttacgccg agcagggtct ggaatttttc tccatcatta
     2521 attccttcgg taagctgacg gattgcgtcg ggctgatcac cggcgggttg aaaaggcgta
     2581 tgtaactgaa aagccatact gcaatttact ttatccaacc ggagtgattg gagggcgtgc
     2641 cggctttatt taatttttct ttgggtatag ccggagtaat cgggcagcag ggtttcatat
     2701 tccgcatgca tcagggggga ggtcagcata aaatcggccg tagaccgatc gcaggccacg
     2761 ggtatattcc aggtagctgc cacccggagc agggccttaa tatcagggtc atggggttga
     2821 gcttccatcg gatcccagaa aaaaatcagg atatcgatct tgccttcagc aatggccgca
     2881 ccgatctgct gatcgccacc aagaggcccg ctgaggaaac aatgtacggg ctgccccaaa
     2941 gctttttcca atagtttacc agtggtgccg gtagcatgta gcgtatgact ttttagcatc
     3001 tgcttattat aaacagccca ctccaccagt tctttctttt tatgatcatg ggcaacgagg
     3061 gcgatgttct tgcactcctt tagtattctt ctttccataa aggaaaatta gttaaaatta
     3121 tggaatcaga tgtcaggtgt gaggtgtcgg gtgtcaggaa acagccccat gaaaccagtc
     3181 gggagttagt agttagtagt cgggagtcaa cagcccttcc taagaaggta gttagtgaac
     3241 agccacttta tgttttttcg tgaatttttg tggcaaaaaa aacggcgctc cgaaggagcg
     3301 ccgctatatg tttctggtat aaactaatta tttcagttct gcagatacgg tgatagccgg
     3361 ttcagtggca attttacctg cttcgatcgg ggcaccggtg ataccataat cagccagttt
     3421 gatgctgaaa gaagtagtgg ctttcaggga accatctttt acttcgaagg ttacagtagt
     3481 ggtaaggggt ttggttacat cgcgtacagt cagttcaccg gtaacgggca cactgtaagt
     3541 acctgcttta tcaaatttta cttttgacag atcagtgatt ttgcctacga atgtgaaggt
     3601 ggggaatttg gcagagttca gccatttttc accattgaaa tggtcctgga tcatcgcatt
     3661 gctgaatgca aagttcttca cgatggcttc gaaaccaacc tggccatttt tggtatttac
     3721 ttcaccaact acggttttgt tatctgcttt aggtaaagca tctttcggtg tagtggcatc
     3781 gaaagagata gtcgcagagc ttgttttttt cacttgcgcg aatacggcag tagaaagggt
     3841 aacgagagag atgataagga atactttttt catgtgtatt gtttgttgtg tttgttgacg
     3901 tttttttatt tgtttatagc ggagcgtttg aagttaatta ccgtagcctg gcgaagccgg
     3961 ctgaatatac cgccactgca ggaggctttg atcgaaacag aatcacctac ggccattttt
     4021 ttaaccgctg ccacatctgc cgcctgaaaa tcaaaaatgg cataggaacc ggaagtggaa
     4081 tctgtaaact taacattgat tgtgctgtcg gctgcttcga gttcggagat acggccatta
     4141 acgacaatga tcttatcggt atatttttta ttggcactac tgtcactgct gataaactcc
     4201 tggatgagtt cggctgcgtt gacggtatag gctggttttt ctttggtggt attggaaaac
     4261 gtttcgtttt ccacctggta cttgacaata ctataaccgc cataggcgag tatcggtccg
     4321 atcagtatcc aggcggtttt caacaaaatg ccatccgggc gactggtcca gataaagagc
     4381 agggcagcca ggccatgaat gtaaagtgag ggttgaatca tcccagagag gctcttaccc
     4441 acacccagat caagtaatgt atgggtaaat atggcataca tagttaccag ggtcagggaa
     4501 agagaaccgg caatcgcggc aatccatcct gcttttttcc ctttgatgtg caggaaggaa
     4561 gccactatgg ttaatacagg aataagtata aacaggtaaa aggtaaaatt gaactgtgga
     4621 tatgggtttg ccagtccgaa tttttcagcg gaagtggcga agaaatgtcc ggtgggtaaa
     4681 tcaagtccgg tcatcttata ttcttcccat tttacccatg gaagaaaaaa gctgaccagc
     4741 atggctgaag cagacagcag gtgaagggaa tttttatgaa ttgttgtctg gggcatatag
     4801 tttcttaaac gatccggacc cttgcttggt tgcgaaccgt taaacaaata tacaatgttt
     4861 aaacattgaa tggttgtttc aaatgtaaat tatttgtgaa gatcccggtg ttttaaagac
     4921 ttatcaggat agggacgcta gcattttatt gtaaatcaat gatatataaa tttaacctga
     4981 aaagcaaaag ggccgcaata aaattacggc cccttactta ctaacccatt aaattaatag
     5041 acaatatggc ttatgtggtc tgcatttcct tgatcttttc gcggatcttt ccttcgatct
     5101 ccttggccag ctccggattg tccaccagga gttgtttcac cgagtcacga ccctgtccga
     5161 gtttatcact gttataactg taccagctgc cacttttctg tacgataccc agttcagttc
     5221 ccatatcgat gatctccccg tttttggaga ttccttctcc gaagatgata tcaaactcag
     5281 ctgccctgaa aggaggagcc actttattct tcaccacttt cacttttacg tggttgccga
     5341 tggcctcatc cccgtctttt acctgttggg tacgacggat atcgagacga acggaggcat
     5401 agaattttaa agcattacca cccgtggttg tttcaggatt accaaacatc accccgattt
     5461 tctcgcggag ctggttgatg aaaatacaga tggtatttgt tttagagatg gtggccgtga
     5521 gtttacgcag ggcctggctc atcagacggg catggaggcc cattttgctg tctcccattt
     5581 ccccttccag ttcactctta ggaaccaggg cggctacgga gtcaatcacc actacatcca
     5641 gggcaccgga aagaatcagc cggtcagcaa tttcaagcgc ctgctcccca taatcaggct
     5701 gggagatcag caggttgtca acgtctacac ccagtttctt ggcataatta ctatcgaatg
     5761 catgttctgc atcgataaag gcacacatac cccctttttt ctgtgcctca gcgatcacat
     5821 gggtggcgat ggttgtttta ccggaagatt caggtccgta gatttcgact acacgaccac
     5881 ggggaagacc accaataccg agggctacat ccaggccaat actaccggtt gaaatgactt
     5941 ccatttctgt aacggctctt tcattcatca tcatcacact tcccttgcca taatccttct
     6001 caatcttatc catagtaagc ttgagggctt tcagtttttc gctgtttgct gacatatcgg
     6061 ttgatttttc ttgttttgcc atttttcaaa attagcggaa tgatctcccg cgggtgtaaa
     6121 aactaattaa caatcaaaac taaatcaatt agcaataaat agctaatatt ttttggatta
     6181 tttttcccgg aaggactgtt tttttccgga cagcaggacg gtgttttatg gattttcatt
     6241 gggaaaaaca ccaggaaatt gggtttttta tggacctatg tctgtcaggc aggagcttgc
     6301 ctaaaaattt agcaaaatct ttttatgtac aatcgggaat gcgggatcag ccaaaaacct
     6361 gggcggccag catcgacaat actcctaagg caaagccacc atagacctct gcccgggtat
     6421 gatctgacac caggaagcgg gccgtgcaaa ccagtccggc gatgaagaca acaagggcga
     6481 gatacaaacc aaagttcagg gcctgtgtaa aggcgagtaa aaaaaggaag gccaccgcaa
     6541 tgcccatgga aatggcatgc aggctgatct tcatccgggt gttcagcatc aggctggtcc
     6601 aggaactgat ccagatagcc agtgcaaaac ggatggccat atccggataa tcgggcaggt
     6661 tatgccaaac ataccatatc cagaaatacc agacgccgca ggctacgagt ggaatgatac
     6721 ggtctttttg ttttttaagt tgaatggatt caataaatcc gagcgccttt aataataata
     6781 cagtaaccaa cgggaagaaa agatacatgg ccaccgcctg aagcatcacc aacatttttt
     6841 gacggggatc gaagccggta aactgaaacg ggtgctcata aatcaggaag gccataacat
     6901 ataccggcac aaataccggg tggaatgccc aggagataaa acgggccatt agccgtaatg
     6961 ccaggggttg atcttcctga ttatttatat gatcgcttga tgctgaagtc agtacagtat
     7021 tcattcttta tattctgcct gcaaagtaac gcaatctgga aaaaagggtg cggtttttgt
     7081 atattggcat cgcgcgacgg cctggtgggg aactattttt ttacaaacac ctcagtatga
     7141 agaaactact gcttgctttt ctaataataa ccggcacatt ccagggattt tcacagaaaa
     7201 tcgacaaggc gaaaatgcag gccatgtacg attccatcaa agcggccggt atccgtcatc
     7261 ccgattttgt catggcccaa tgtatgcagg aaaccggcaa cctgaaatgc aaacaatgct
     7321 gcctacgcta ccataacctc tttggttttt atataaaagg aaacaagtgc aaaaaatttg
     7381 aatccgatgc ggcctgcatc gctt
//
LOCUS       DAOPMW010000139         7355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11416_length_7355_cov_8.539041, whole genome shotgun sequence.
ACCESSION   DAOPMW010000139 DAOPMW010000000
VERSION     DAOPMW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11416_length_7355_cov_8.539041"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1873
                     /locus_tag="PLU11_07990"
     CDS             173..1873
                     /locus_tag="PLU11_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59097.1"
                     /translation="MLPLLQINHLSIDFSTEAGLTHALRDINITVNRGEILALVGESG
                     SGKSVTSLSILQLLPSPPAIYSSGEIIFSDDGQSSANLLKRDRYGMQDIRGNKIAMIF
                     QEPMTSLNPVLTCGNQVMEALLLHKKGSRAEARQRTIAWFEKVKLPDPEGIFNRYPHQ
                     LSGGQKQRVMIAMAMCCEPSLLICDEPTTALDVTVQKTILELIKELQQQSGMGVIFIT
                     HDLGVVAEIANRAAVMYKGEIVEQGATEQLFLSPVHPYTKALLACRPVNHQRGERLPV
                     VADFMEPGREKAVKSALAPVKENKSEILLSAQKLSVWFPVKKNMLGKALLYNKAVNQV
                     SFDVYKGETLGLVGESGCGKTTLGRALLRLIEPTAGKIIYNQTDLTARKKESLKELRK
                     EIQIIFQDPYSSLNPRLTIGAAIAEPMKVHQLHSTDKQRKDKVLELLEKVNLRPEHFN
                     RYPHEFSGGQRQRIVIARALALNPSFIVCDESVSALDVSVQAQVLNLLNDLKKEFGFT
                     VVFISHDLSVVRYISDRIMVMNKGQIEESGAADDIYLHPRSAYTQKLISSIPKGITTG
                     "
     gene            complement(1863..5714)
                     /locus_tag="PLU11_07995"
     CDS             complement(1863..5714)
                     /locus_tag="PLU11_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPN59098.1"
                     /translation="MKRLTLLLLSVFSVFIIQAQTIKKEKKYPSLLWEITGNGVKNPS
                     YLIGTMHVSSKMAFNLPDSFYLAVKSAQVVALETNPETWQEDMSKYDLEGGMRGSSNN
                     WGGSISGPTDYLNIKTLKFFKYDGKLERSMFSNPSTINNLLYRSYGNESADFEEDTYL
                     DMYIYQCGKKWGKKVAGVEDYGESMRLMMEAYKDAAKDKTRKERSYGDMDEEYGADKL
                     QEAYRKGDLDLLDSINMYNSFSAAFDEKFLYRRNEIQAGSIDSIIKSGSVLFVGVGAA
                     HLPGDRGVIEMLRAKGYKLRPVKMGERASKTKDLVDKLRVPVQFKTVQSDDGFFKVDI
                     PGKFYTSGEEGSLDQQQYADMANGSYYMVTRVMTNAWLWNNSPEDVLKKVDSLLYENI
                     PGKIISKTPVLKNGYKGYDIVNRTRRGDMQRYHIFVTPFEVLFFKMSGNSDYVKGGDE
                     AKRFFGSIQLKEYKPATEPAGNWKKFSPAYGGFAVDLPHLPYTGNDGSWIYDAEDKST
                     ATHYRVIRTDIHNYGFAEEDTFDLALMEESFMASEFIDSSLHRKHTVYKGYPVLEAKY
                     RDKQGGLFDVRYLIQGPHYYTLVAHGKQDQPEMQRFLQSFEIKPLQYRAAKTEKDTAL
                     YFSVQTPVFPEEKKIKLDMSPDDYSDGEEAETEAEMLENGTFRGKLIENDTTGEKIHV
                     VFYKSSRYHYSKDSSRLTQDRGFYYKGDSTWIIRTYKKTDLPGNRKVWDILVSDTGSS
                     RAIRYKAYYKDGVTFTLTTLTDTLTPPSDFVKQFYESFTPVDTLKGLNPFTKKAALFF
                     DDLRSKDTVVRKKAISNIGEIYLDSTDLPELMNAIMACNWEQKKYLNTKKELISKLGE
                     IKTTAAADNLRTLYFSLGDTVQLVYPVLQGLLGHQNDYAYGIFRNIIANEPPVLDFSG
                     DYNYNYARTLYQINKSYGMDRYYGDGNFLDRLSDSLALTKTILPDLLPLLNLEDYKSP
                     VMDLLGEMADSNLLKPSDYVMYYNKFLLEAKQELKKQSIAEKKAAIEKAEASKEDKKG
                     RENEEDEPDDGNDLLTQYAKLLIPFWDSHPAVSPLINQMLASNDKKLKYNILLQLMKM
                     GRSYPDSLLRYFGGLDEYRYELYSDLKEMKKTEKFPAMYNNHLDLGKSSLLRLKSGDK
                     PDSLVYADRISTTYKGKKGYIYFFNYKMKKDDLNWKMAVVGLVPENPREFEFEDTIHR
                     PVVFYNYGEQRRWPGSDTYDFTRYTDEKTNTGEAMKVQMRKELKKMIYERRESALRFY
                     NNNYNRGYGFNMHEPEFQLSR"
     gene            6031..7080
                     /gene="queA"
                     /locus_tag="PLU11_08000"
     CDS             6031..7080
                     /gene="queA"
                     /locus_tag="PLU11_08000"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPN59099.1"
                     /translation="MKLSQFRFDLPLNLIAQTPTKRREDSRMMVVHRHTGEIENKHFR
                     DIMDYFDDKDCFVVNNTKVFPARMYGRKEKTGAKIEVFLLRELNKPNRLWDVIVDPAR
                     KIRVGNKLYFGDQEDLVAEVIDNTTSRGRTIRFLWEGSEEDFRAQLEKLGETPLPKYI
                     KRKPDEDDKERYQTVYAKHEGAVAAPTAGLHFSRELIKRLEIKGIRFAEVTLHTGLGT
                     FRPIEVEDLSKHKMDAEYYNIDETACKVVNRAKEYGHRVCSVGTTTMRALESSFTAQK
                     LLKPSEGWTNMFIHPPYDFSIADSLVTNFHLPKTSLLIMTCAFAGYELAMEAYKKAIK
                     DKYRFFSYGDALLII"
BASE COUNT         1919 a         1826 c         1638 g         1972 t
ORIGIN      
        1 tcttactggt cagtgatcag cccatgattc cggaaggcgt aaagacagaa gaaagcgata
       61 aaaaagtaac ggccagtttt gtggaaaacc atttaaagat tggcatagat tctctgaaac
      121 aactgatcaa tgatggacat accgtacgtc acctgaaatt ctgatatccc tgatgctgcc
      181 cctgctccaa attaatcacc tgagtattga tttcagtacc gaagccggac taacacatgc
      241 actcagggat attaatatca cggtcaaccg gggggagata ctggctttag taggagaatc
      301 cggttcagga aaatccgtca cgtccctttc catactgcaa ttgcttcctt cgcctcctgc
      361 tatttacagt tcgggagaaa ttattttttc tgacgacggc cagtcatccg ccaatttgct
      421 gaagcgtgac cgctatggca tgcaggatat ccgcggcaat aaaatcgcga tgatcttcca
      481 ggaaccgatg acttcgctga acccggtact cacctgtggc aatcaggtga tggaagccct
      541 gctgttacat aagaaaggaa gccgggccga agccaggcaa cggaccattg cctggttcga
      601 aaaagtgaaa ctacctgacc cggaaggaat attcaaccgt tatccgcacc agttaagtgg
      661 cgggcaaaaa cagcgggtga tgattgcgat ggccatgtgc tgtgaaccat ccctgttaat
      721 ttgtgatgaa ccaaccacgg ccctggatgt tactgttcag aaaacgatcc tcgaactcat
      781 taaagaatta cagcagcaat ccggaatggg ggtcatcttc atcacgcatg acctgggggt
      841 ggtggcagag atagccaacc gggctgccgt gatgtataaa ggagaaattg tcgaacaggg
      901 tgccacggaa cagctattcc tttcaccggt tcacccgtat accaaagcct tattggcctg
      961 tcgtccggtg aaccatcagc gcggtgaacg actgcccgtg gtggcagatt ttatggagcc
     1021 tggccgggaa aaggcagtaa aatcagccct ggctccggta aaagaaaata aatcagaaat
     1081 tttattaagt gcccaaaagc tatcggtatg gtttcccgtg aagaaaaata tgctgggcaa
     1141 agccctgctc tataacaaag cggtcaacca ggttagtttt gacgtttaca aaggagaaac
     1201 acttggtctg gttggcgaaa gcggttgcgg gaaaacaaca ttgggacggg cattgctccg
     1261 gttgatcgaa cccacggcgg gtaaaatcat ttataatcag acggacctga cggcacggaa
     1321 aaaagaaagc ctgaaagagt tgcggaaaga aatacaaatc attttccagg acccctactc
     1381 ttcattgaat ccaaggctga cgatcggggc tgccattgca gaaccgatga aagtgcatca
     1441 gttgcattcc accgataaac aaagaaagga taaagtgctg gagctgttgg aaaaagtaaa
     1501 tctccggccg gaacatttta accgctatcc gcatgaattc agcggcgggc aacggcaacg
     1561 gatagtgatt gcccgggcac tggccttgaa tccttccttt atcgtttgtg atgaatccgt
     1621 atctgccctg gatgtaagcg tgcaggctca ggtgctgaac ctgctcaatg acctgaagaa
     1681 agaattcggg tttaccgtgg tctttatttc ccacgacctt tcggtagttc gttatatcag
     1741 tgaccgtatt atggtgatga ataaaggcca gatagaagaa agcggggccg cagatgatat
     1801 ttacctgcat ccccgctccg cttatacaca aaaactgatt tcatccattc ccaaaggaat
     1861 cactaccggc tgagctgaaa ttccggctca tgcatattga aaccgtaacc acggttataa
     1921 ttattgttat aaaaccgtaa agcactttcc cttctttcat agatcatttt cttcagctcc
     1981 ttccgcatct ggaccttcat agcttcccct gtattggttt tttcatccgt ataccgggta
     2041 aaatcatatg tatcgctacc gggccagcgg cgctgctccc cgtaattata aaaaacgaca
     2101 ggccggtgaa ttgtatcttc aaattcaaac tcacggggat tctccggcac cagtccgact
     2161 acggccattt tccagttaag atcatctttc ttcattttat aattgaagaa atagatatac
     2221 cctttcttcc ctttatacgt agtactgatc cggtccgcgt agaccagtga atcgggcttg
     2281 tcgccggact tcaaccggag taaggaactt ttacccaggt ccaggtgatt gttatacatc
     2341 gccgggaact tctccgtttt tttcatttct ttcagatcgg aatacaactc gtagcggtat
     2401 tcatccaatc ctccgaaata acggagcagg ctgtccggat aagaccggcc catcttcatt
     2461 agctgcagca ggatattata cttcagcttt ttatcattcg atgccaacat ctggttgatc
     2521 agcggagaca ccgccggatg gctgtcccag aaaggaatca gtaatttggc atactgggtc
     2581 agcagatcat tcccgtcatc cggctcgtcc tcctcattct cccttccttt cttatcttct
     2641 ttactggctt ccgctttttc aatcgctgcc ttcttttccg caatcgactg ttttttcaat
     2701 tcctgtttgg cttccagcag gaacttatta taatacatta cataatcgga aggcttgagc
     2761 agattgctgt ccgccatttc gcccaacagg tccatcacgg gagatttata atcctccagg
     2821 ttcagcaagg gcaacagatc agggagtatg gtttttgtca gggccagtga gtctgataaa
     2881 cggtccagga aattaccatc cccgtaatac cggtccatcc catagctttt attgatctgg
     2941 taaagagtcc gtgcatagtt ataattataa tcgccactga aatccagaac cggtggttcg
     3001 ttggcaatta tgttccggaa tattccatat gcataatcat tctgatgccc gagcaaaccc
     3061 tgcaacaccg ggtataccaa ctgtacggta tctcccagcg aaaaatacaa tgtacgcaga
     3121 ttgtcagcgg ccgccgtggt tttaatctcc ccgagtttac tgatcagttc ctttttggta
     3181 ttcaggtatt ttttctgctc ccagttgcag gccataatgg cgttcattaa ctccggcaga
     3241 tccgtggagt ccaggtaaat ttccccgata ttgcttattg cctttttccg aaccacggta
     3301 tccttgctgc gcaggtcatc aaaaaacagg gcagcttttt ttgtaaaagg attgaggcct
     3361 ttgagcgtat ccacgggtgt aaagctttca taaaactgtt tcacaaaatc actgggtgga
     3421 gtcagggtat ctgtcagggt ggtcaaggta aatgttaccc cgtctttata ataggcctta
     3481 tacctgatcg cacggctgct gccggtatcg gacaccagga tatcccatac tttccggtta
     3541 cctggcagat ctgttttttt atacgtccgg atgatccagg tggaatcccc cttataataa
     3601 aacccacggt cctgcgtcag ccggctgctg tctttcgaat agtgataccg ggatgactta
     3661 taaaaaacca catgtatttt ctctcccgtg gtatcattct caatcagctt accccggaaa
     3721 gttccgttct ccagcatctc cgcttccgtc tctgcttctt ccccgtctga ataatcatcc
     3781 ggcgacatat ccagtttaat ttttttctcc tccgggaata ccggtgtctg tacactgaag
     3841 tacaaagcgg tatctttctc cgtttttgcg gccctgtatt gcaatggctt aatttcaaaa
     3901 gactgcagga agcgctgcat ctccggttga tcctgtttgc cgtgtgccac cagtgtataa
     3961 taatgaggtc cctggatcaa ataacggaca tcaaaaagac ccccttgttt gtcgcggtac
     4021 tttgcttcta ataccggata gcctttgtaa accgtatgtt tccggtgcaa tgaactgtct
     4081 ataaattcag aagccatgaa actttcttcc atcagggcca ggtcaaaagt gtcttcctct
     4141 gcaaaaccat aattatggat atcggtacgt attacacggt aatgtgtagc cgtgctttta
     4201 tcttccgcat cgtagatcca gctgccgtca tttccggtat agggcaaatg gggaaggtca
     4261 actgcaaatc ctccataagc cggcgaaaat ttcttccagt tgccggcggg ttcagttgcc
     4321 ggcttgtact ccttcagttg tatgcttcca aaaaaacgct tcgcttcgtc tccgcctttt
     4381 acataatcac tgttcccgct cattttaaaa aacagcactt caaatggcgt gacgaaaata
     4441 tgataccgct gcatatctcc ccggcgggtc cggtttacaa tatcataacc tttgtacccg
     4501 tttttcagca ccggggtttt ggaaataatt ttaccgggaa tattctcata aagcagactg
     4561 tccacttttt ttaacacgtc ttccggactg ttgttccata gccaggcatt ggtcataaca
     4621 cgggtgacca tataataact gccattggcc atatccgcat actgttgctg atccagcgaa
     4681 ccctcttcac cggatgtata aaatttcccg ggaatatcga ctttaaaaaa accatcatcg
     4741 gattgtacgg ttttaaattg aaccggcacc cgcaacttgt ctaccaggtc cttggtttta
     4801 ctggcccgct cgcccatttt caccggacga agtttatacc cttttgcacg gagcatttca
     4861 attacccccc ggtcaccggg caggtgggca gctcccactc ctacaaagag gacactgccg
     4921 gacttaataa ttgaatctat tgaaccggcc tgtatttcat tccggcgata aaggaatttc
     4981 tcatcaaagg ctgccgaaaa actgttatac atattaatgg aatccagcaa gtcaagatct
     5041 cctttccggt aagcttcctg tagtttatcc gcaccgtatt cttcgtccat atccccgtaa
     5101 ctccgctctt tccgggtttt atctttagcc gcatctttat aggcttccat catcagccgc
     5161 atgctttctc cgtagtcttc caccccggcc actttcttac cccatttctt tccacattgg
     5221 tagatataca tatcgagata ggtgtcctct tcaaaatcag ccgattcatt gccgtaagac
     5281 cggtaaagca gattgttaat ggtactggga ttgctgaaca tcgatcgttc gagtttgcca
     5341 tcatacttga aaaacttgag cgtcttaata ttcaggtaat cagtggggcc ggagatggag
     5401 cccccccagt tattggaact gccacgcatc cccccttcca ggtcatactt actcatatcc
     5461 tcctgccagg tttccggatt ggtctccagg gcaaccacct gtgcactttt aacggcgagg
     5521 taaaacgaat ccggcaggtt gaaagccatc ttgctgctga catgcattgt gccgatcagg
     5581 taagaaggat ttttgacccc gttgccggta atttcccata ataaactggg gtatttcttt
     5641 tcttttttaa tggtctgcgc ctgaataata aatacagaga agacagaaag aaggagtaag
     5701 gttagtcgct tcatatcagg gatttcccgg taataccggg gctttttgtg atgaacaaat
     5761 gtaagatgcc gaaatccaat aaaagcataa aatgatcagt atgagggggc taaagggaaa
     5821 atgccccaaa cgccggtttt ggagtgccgg attcagataa atgcccggtt ttcgccgaaa
     5881 aaccgtaatt ttgcccgccg ccaaaggagc atatccggcg gtattgaaca gtcgctttga
     5941 ccagacccgg cgccgtaagg ccggcaagga agagccccgg cgtatcgcca ggtttgcgtc
     6001 atgagactat cgtactaaac ccaaacactc atgaagcttt cacaattccg attcgacctt
     6061 cccttaaacc tcattgcaca aacacccacc aagagacgtg aagattcccg catgatggtg
     6121 gtacatcgcc acaccgggga gattgagaac aagcatttcc gcgacatcat ggattatttt
     6181 gatgacaagg attgttttgt agtaaacaat accaaggtgt tccccgcccg tatgtatggc
     6241 cgtaaagaaa aaaccggggc caagatcgag gttttcctgc tccgggaatt aaacaagccc
     6301 aaccgccttt gggacgtaat cgttgatccc gcaagaaaga ttcgtgtagg taataaactt
     6361 tatttcggag accaggaaga cctggtagcg gaagtcattg ataataccac cagccgtgga
     6421 cgtacgatcc gtttcctctg ggaaggatcc gaagaagatt tccgcgctca actggagaaa
     6481 ctgggtgaaa cacccctccc caaatacatc aaacgcaaac cggacgaaga tgataaagag
     6541 cgttaccaga cggtttatgc caagcatgag ggagcggttg cggctcctac agccggactg
     6601 catttcagtc gtgaactgat caagcgcctg gagattaaag gcatccgttt tgctgaagta
     6661 accctgcata ccggattggg aactttccgt ccgattgaag tggaagacct cagcaaacat
     6721 aaaatggatg cggaatatta taatattgat gagacggcct gcaaagtggt caaccgcgcg
     6781 aaagaatacg gacatcgggt ttgttccgta ggcaccacca ccatgcgtgc cctggaatca
     6841 tctttcactg ctcagaagtt gttgaaacct tctgagggct ggaccaatat gttcatccat
     6901 ccgccttatg atttcagcat cgcagattcg ctggtaacca atttccacct gcccaagacc
     6961 agcctgctaa tcatgacctg tgcctttgcc ggttatgaac tcgccatgga ggcctataaa
     7021 aaagcgatca aggataagta ccgtttcttc agctatggag atgcgttgct gatcatttaa
     7081 gcaaatacca actgtttttc gcgagccgcc tgtgagggcg gcttttttta ccacaccccc
     7141 ttagtatcta tccaaaaatc agagactccg ctttccccac tccttaagga gaggggaagc
     7201 ggagtcttca atttgaatga tttttctaag ggggtgaggt tactccagcc cgaacatccc
     7261 tttatttaat tgctgcaata tctgcacttt atgtccggca tccgtaagca gggaaatatt
     7321 gtgcggactt ccaccatagc tgaccatccg tacgg
//
LOCUS       DAOPMW010000140         7292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11555_length_7292_cov_10.131684, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000140 DAOPMW010000000
VERSION     DAOPMW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11555_length_7292_cov_10.131684"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLU11_08005"
     CDS             <1..206
                     /locus_tag="PLU11_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPN59100.1"
                     /translation="WRIKVTGLLHNRMAYAEAIKYYIDITPEEELQRIQYQAATFHTG
                     KRRSKIGRPTKKERRELDDFLDD"
     gene            complement(203..646)
                     /locus_tag="PLU11_08010"
     CDS             complement(203..646)
                     /locus_tag="PLU11_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN59101.1"
                     /translation="MFFSKSFGYALRGVLFVALIHQEGRRVQLDEMAEKLQVPRHFLA
                     KIMKLMVKEGILHSTKGPYGGFSLTDHSLNKKIIHIVRITDGVEQFNTCVLSLRKCKN
                     DNPCPLHYQMMKLRDDMLAEFSARTLGDLLDEKNPNFIRSISTLA"
     gene            912..1382
                     /locus_tag="PLU11_08015"
     CDS             912..1382
                     /locus_tag="PLU11_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPN59102.1"
                     /translation="MKRNLILSTVLLSAACLITSCNEGVKEDPKPIDVQELTKDQPET
                     HGSEIKEGDITLSTPLDKAMVDAGKATYGLKCQSCHKLTEEKLVGPGWMGVTKKRQPV
                     WIMNMITNVDMMLEKDPEAQKLLELCLVRMPNQNISKDEARQVIEFMRSNDGEK"
     gene            1443..>2758
                     /locus_tag="PLU11_08020"
     CDS             1443..>2758
                     /locus_tag="PLU11_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HPN59103.1"
                     /translation="MKHSFLKLLTATLLLAVGFSGLTSCKPKGTQSAAGGDAAKAYVA
                     PGKYDEFYSFVSGGFSGQMSVYGLPSGRLFRVIPVFSVDPEKGWGYNEETKPMLNTSH
                     GFVPWDDLHHIALSVTKSEHDGRWAFGNANNTPRIARIDMKTFRTTEIIEIPNSAGNH
                     SSPFITENSEYVVAGTRFSVPMGDNQDVPINTYKENFKGTVSFIGIDKTSGEMNVSFQ
                     IATPGINFDLARAGKGRSAGWFFFSCYNSEKANTLLEVNASQKDKDFIMAVNWKKAEE
                     YAKAGKGVKKQVRYAHNKYNESTHTATSEIKNEVLMLDPKDCPDMVYFMPCPKSPHGC
                     DTDPTGEYIVGSGKLAAVIPVFSFAKIEAAIAAKNFDGSYDGIPVLKYEAVLHGEVQK
                     PGLGPLHTEFDGKGNAYTSFFVSSEIVKWSVKDLKVLDRVPTYYSIG"
     assembly_gap    2759..2858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2859..3314
                     /locus_tag="PLU11_08025"
     CDS             <2859..3314
                     /locus_tag="PLU11_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HPN59104.1"
                     /translation="YKLEDNKHPYAALGDDNAKVVRKGNEIHVYMTSIRSHFTPDNIE
                     GVKLGDIVYFHVTNLEQDWDVPHGFAVKGIANAELLIMPGETQTLAWKPEKAGVYPMY
                     CTDFCSALHQEMQGYIRVSPAGSNVPLTFSTGTNLPATTPAATGAPSTK"
     gene            3374..3937
                     /locus_tag="PLU11_08030"
     CDS             3374..3937
                     /locus_tag="PLU11_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN59105.1"
                     /translation="MKKIFNLMLFSSILLMISCNNNTSTETASSTADAPAAGGQEAVQ
                     DDVSQKDVVKVAVGSPDHTTLVAALKQAELVTSLANAGPFTVFAPTNAAFEKLPAGTV
                     DGLMKDDKKADLQNILQYHVTTSALDLKFLTDGMTLGMVNGDNLTITVKDGKTILNGS
                     ATIIGSVRGSNGWVHVIDGVLLPPAKK"
     gene            4011..4601
                     /locus_tag="PLU11_08035"
     CDS             4011..4601
                     /locus_tag="PLU11_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59106.1"
                     /translation="MKNNLSITSRILVAFASGALIAVFFLPAWRIDLFAPQYPEGLMM
                     NIWINGLSGDVEIINGLNHYIGMKHISVDMFPEFKYLPWVVGFFMLLGMIVAITGKRK
                     FLLYYLILTVIGGALAVWDFYQWGYDYGHNLDPKAAIQVPGLSYQPPLFGHKRLLNFD
                     AYSFPDVGGWVVIGASALAFSVWFTEWYRHRKKQIV"
     gene            4598..5041
                     /locus_tag="PLU11_08040"
     CDS             4598..5041
                     /locus_tag="PLU11_08040"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HPN59107.1"
                     /translation="MKPISITLYGLFFLLLTSCSTKPEPFAYGKDNCHTCKMGIMDPK
                     FGTELITTKGKIYKFDDVSCMQYFLKSNTLEPKEISQQLVINFEKENDFLSLEEAVFI
                     LSPSVKSPMGSHVAAFASREKAEKMNATFTGELLSWKELAGKLDQ"
     gene            5041..6273
                     /locus_tag="PLU11_08045"
     CDS             5041..6273
                     /locus_tag="PLU11_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HPN59108.1"
                     /translation="MKKILTFFCLIISLGLFAGQIVVGTAAPVKSLRKAIEMARPGDT
                     IRLQAGLYKEGSLVISKPLTLLGENNPVLDGESKYEILLISGRDITVRGITFRNSGYS
                     AMNDFASIKLVDCTNITLEKNTILNAYFAIHISNSVNAIVRYNRIFGIPASEQLTGNG
                     IHLWKSNHAVIENNQVEGHRDGIYFEFVTASTISNNLSQKNIRYGLHFMFSNDDTYRN
                     NTFRNNGAGVAVMYSKKVRMEKNHFEENWGASAYGILLKDITDSYIRQNQFVKNTVGI
                     HMEGSSRIEIRENQFKNNGWALKVQASCDDNFFHHNNFTGNSFDIGTNGTLVLNKFEK
                     NYWDKYEGYDMNKDGIGDIPYHPVSMYSMIVEENPTTLILFRSFMVSLLDKAEKAIPS
                     LTPENLYDNQPMMKMLSL"
     gene            6270..7034
                     /locus_tag="PLU11_08050"
     CDS             6270..7034
                     /locus_tag="PLU11_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59109.1"
                     /translation="MIHIEAVYKKFRRLQVLENISARFTAGQVIALIGPNGSGKTTLI
                     KSILGMVRPDKGTIRVKDHLVTGNPDYRQFIGYMPQIGRYPDNMKVGQLFEMMKKIRG
                     IESVDIDLLTRFEIEKIYEKPMRTLSGGTRQKVSAALAFLFDPPILILDEPTAGLDPL
                     SSEILKEKIIQEKKKNKLILITSHVLSDLEELTSHIMYLQEGKLQFIKDLETLQEETG
                     EIKLSKAIAKLMRGQETSSAWIDQLFAVEQNKENPQ"
     gene            7045..>7292
                     /locus_tag="PLU11_08055"
     CDS             7045..>7292
                     /locus_tag="PLU11_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN59110.1"
                     /translation="MLKLSRYVLYDILRNKIIIAYTLFLLLVSLSMFRMEENGSKAML
                     SLLNIVLIVLPLVSLVFTTIHYYNSYEFIELMLSQPLSR"
BASE COUNT         2103 a         1624 c         1581 g         1884 t
ORIGIN      
        1 gctggcggat caaagtgacg ggcttgctgc ataaccggat ggcctatgcg gaagcaatca
       61 aatattatat cgatattacc ccggaagagg aactccagcg tatccagtac caggctgcca
      121 cttttcatac cggcaaaaga agaagtaaga tcggcaggcc gaccaaaaaa gagagaagag
      181 aattagacga tttcctggat gattaagcca gggtggatat gctccggatg aaattcggat
      241 tcttttcgtc gagcaggtcc ccgagtgtac gggccgaaaa ctcagccagc atatcatcgc
      301 gaagtttcat catctgatag tggagggggc atggattgtc atttttacat tttcgcaaac
      361 ttaagacaca ggtattaaat tgttccacac catcagttat ccttactata tgtataattt
      421 ttttatttaa tgaatgatca gttaatgaaa aaccaccata aggccccttc gtggagtgaa
      481 gaatcccctc ctttaccatc agtttcatga ttttagccag aaaatgacgg ggaacttgca
      541 gtttttccgc catttcgtcc aattgaactc ttcgaccttc ctgatgtatc aatgctacaa
      601 aaagtacccc gcgaagggca tatccgaatg attttgagaa aaacatatat ttttgaataa
      661 ttgccaaaga tatcgggtat attaccttat tgcactgacc agtatcaacc attgaaatga
      721 tgccggtcac atacctcctg acattacctg gaagaaattt agcccctaaa ggtgacaact
      781 atcacaatag tgattgataa agatcagaac ccgccctttt tccggaaaat acatttgcgt
      841 cacaaaagac ataagtatct tttttaactt tagaagtaca acttcattta actcaacaaa
      901 aaaaacaaat catgaaacgc aatctgattc tctccaccgt gctgctaagt gcagcctgct
      961 taatcacttc ctgcaacgaa ggggtaaaag aagacccgaa gcccattgat gtgcaggaac
     1021 tgaccaagga ccagcccgaa acccatggct ccgaaatcaa agaaggagat attaccctta
     1081 gtactccgct tgacaaagcc atggtcgatg ccggaaaagc cacgtatggc ctgaaatgcc
     1141 agagctgtca caaactcact gaagaaaaac tggttggccc gggctggatg ggtgtcacta
     1201 aaaaaagaca accggtctgg atcatgaata tgatcaccaa tgtagacatg atgctggaaa
     1261 aagaccccga agcacaaaaa ctcctggaac tctgcctggt gcgcatgcct aaccagaata
     1321 tcagcaagga tgaagcccgc caggttatcg aatttatgcg cagcaatgat ggcgagaaat
     1381 aaaaatacat gaaacaaaac caccggattc ccattcaatt tcttaactgc taaatattaa
     1441 acatgaaaca ctcttttcta aaattactga cggccaccct tttattggcg gttggttttt
     1501 ccggacttac cagctgtaaa cccaaaggta cccaatcggc tgccggtgga gatgcggcta
     1561 aagcatatgt agctcccggt aagtatgatg aattctacag ttttgtttcc ggtggcttca
     1621 gtggacaaat gtctgtctac ggtcttccct ccggtcgttt attccgggta atacccgtat
     1681 tctctgtgga ccctgaaaaa ggatggggct ataatgaaga aactaagccc atgctgaata
     1741 cctcccatgg atttgtaccc tgggatgatc tgcaccatat cgctctttcc gttaccaaaa
     1801 gcgaacatga tggccgctgg gcttttggaa atgccaataa caccccgcgt attgcccgga
     1861 tcgatatgaa aacattccgg accactgaaa tcatcgaaat ccccaatagt gccggtaacc
     1921 actcctctcc ctttatcact gaaaattctg agtatgttgt agcgggtaca cgatttagtg
     1981 ttcccatggg tgacaaccag gatgtgccga taaatactta taaagaaaat ttcaaaggaa
     2041 cagtcagctt tattggtata gacaagactt ccggtgaaat gaatgtctcc ttccagattg
     2101 ccactcccgg aatcaacttt gacctggccc gtgccggtaa aggcagatca gccggctggt
     2161 tcttctttag ctgttataat tctgaaaaag ctaataccct tctggaagta aatgcttctc
     2221 agaaagacaa ggacttcatc atggccgtta actggaaaaa agcggaagag tatgcgaaag
     2281 ccggtaaggg cgtgaaaaag caagtccgtt atgcccataa taaatacaac gaatccaccc
     2341 atactgccac ttccgaaatc aaaaatgaag tgctgatgct ggatcccaag gattgcccgg
     2401 atatggtcta ctttatgccc tgccccaaat ccccgcacgg ctgtgatacc gatcctaccg
     2461 gtgaatatat tgttggtagt ggcaaactgg ctgccgtaat ccctgtattt tctttcgcca
     2521 aaattgaagc cgctattgcc gctaaaaatt ttgatggcag ttatgatggc attcctgttt
     2581 tgaaatatga agccgtgtta catggagaag tccagaaacc cggactcggg cctttacaca
     2641 ctgaattcga tggtaagggc aatgcttata cttcattctt tgtatcttca gaaatcgtga
     2701 aatggagtgt gaaggacctg aaagtactgg atcgtgtacc tacatactat tctattggnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta taaactggaa gacaacaaac
     2881 acccttacgc tgctttgggt gatgataatg ccaaagtggt gcgtaaagga aatgagatcc
     2941 acgtgtacat gacttccatc cgttcgcact ttacgccgga taatattgaa ggcgtgaagc
     3001 tgggtgatat tgtttatttc cacgtaacca acctggagca ggattgggat gtccctcatg
     3061 gttttgccgt aaaaggaatc gccaatgcag aattactgat catgcccggc gaaacacaga
     3121 ccctggcctg gaaaccggag aaagcaggtg tttatccgat gtactgtaca gacttctgtt
     3181 cagctctcca tcaggaaatg cagggttata tccgggtcag tccggccggt tctaatgtac
     3241 cgttgacttt cagtacagga actaacctgc ctgccactac tccggcagca accggagcgc
     3301 cgtccaccaa ataatcatca tcagcggtct ggcttaaaac cggaccgctg attcatcaac
     3361 ccaaactgtt tttatgaaaa agatatttaa cctgatgctc ttcagcagta tattactgat
     3421 gataagctgc aataataata catccacaga aactgcatct tccactgcag atgcccccgc
     3481 tgctggcgga caggaagcgg ttcaggatga tgtatcacaa aaagatgtgg taaaagtagc
     3541 ggtgggctct cctgatcata ctacactggt cgctgccctg aagcaggcgg aactggttac
     3601 ttccctggcg aatgcaggtc ctttcactgt ttttgcacct accaatgcgg cctttgaaaa
     3661 actccccgcc ggtacggtgg atggattaat gaaagacgat aaaaaagcgg acctgcagaa
     3721 tatcctgcaa taccatgtta ccacttcagc actggattta aaattcctca ccgatggcat
     3781 gacgttaggt atggtgaatg gagataacct gactattact gtaaaagatg gcaaaacaat
     3841 actcaacggt tctgctacga tcattggttc agtaaggggc tccaacggat gggtgcatgt
     3901 gatcgatggc gtattacttc ctcctgctaa aaaataattc agcttttgcg tttcaatgat
     3961 tccctgatgg cttccgggac aagtgcccgg aagtctttta attaaacagt atgaaaaata
     4021 atctcagtat cacgtcccgg atcctggttg cctttgcttc tggtgctctt attgctgtct
     4081 ttttccttcc cgcctggcgg atcgatcttt ttgcaccgca atacccggaa gggttgatga
     4141 tgaatatctg gatcaacggg ctttccggcg atgtggagat catcaacgga ctgaatcact
     4201 atattggcat gaaacatatc agtgtggata tgtttcctga atttaaatat ctcccgtggg
     4261 tagtaggttt ctttatgctg ctcggaatga tcgtggctat tacaggtaaa cgtaaattcc
     4321 tgttgtatta tctgatattg actgttattg gcggtgcatt agccgtctgg gacttttacc
     4381 aatggggtta cgattacgga cataacctgg atcccaaagc ggccatccag gttccgggac
     4441 tctcctatca gccaccatta ttcggccata aacgtttgct caacttcgat gcttattcct
     4501 ttccggatgt aggtggctgg gtagtgatcg gtgcatctgc gctcgctttc tctgtttggt
     4561 ttacagaatg gtatcgtcac cgtaaaaaac aaatcgtatg aaaccaatta gcataacact
     4621 gtacggcctg ttctttctgc tattaaccag ctgcagcaca aagcccgagc cttttgccta
     4681 tggaaaagac aattgccata cctgtaaaat gggaattatg gatccgaaat ttggcactga
     4741 actgattact accaaaggaa agatctacaa atttgatgat gtatcctgta tgcaatattt
     4801 tctgaaatcc aatacccttg aaccaaaaga aatcagccag caactggtga ttaactttga
     4861 aaaagaaaac gattttcttt ccctggagga ggccgtattt atcctgagtc cttcggttaa
     4921 aagtccgatg ggtagtcatg tcgccgcttt tgccagccgg gaaaaagcgg aaaaaatgaa
     4981 tgcaacattt actggtgaac tactgagctg gaaagaactg gctggtaaac tggaccaata
     5041 atgaaaaaaa tactgacctt tttttgtttg attatttccc tgggattgtt tgccgggcag
     5101 atcgtcgtgg ggacggctgc tccggttaaa tcccttcgga aagccataga gatggcccga
     5161 cccggagata ccatccggtt acaggcaggc ctgtataaag aaggtagtct ggtgatttcc
     5221 aagcccctta ccctgctcgg cgaaaacaat cctgtcctgg acggagaaag caaatatgag
     5281 attctgctga tcagtggcag ggacataacc gtacggggta ttacattccg gaattccggg
     5341 tattctgcta tgaacgattt cgcttccata aaactggtgg attgtaccaa tataacactg
     5401 gaaaaaaata ccatcctcaa tgcctatttc gcgatccata tttccaatag tgtaaatgcc
     5461 atcgttcggt ataaccggat ttttggcatc ccggcttctg aacaattaac gggtaatggc
     5521 atccatctct ggaaatcaaa ccatgcagta attgaaaaca atcaggtgga aggacatcgg
     5581 gatggcattt actttgaatt tgtaaccgcc tccactatca gcaataacct gagccagaaa
     5641 aatattcgct acggactgca ttttatgttt tccaatgatg acacttaccg gaacaatacc
     5701 tttcggaata atggtgccgg ggtggccgtg atgtattcca aaaaagtacg gatggagaaa
     5761 aatcattttg aagaaaactg gggcgcttcc gcttatggca tcctgttgaa agatatcacc
     5821 gacagttata tccggcagaa ccagtttgta aaaaacaccg tgggcatcca tatggaaggc
     5881 agcagccgga tcgagatccg ggaaaatcag tttaaaaata acggatgggc ccttaaagtg
     5941 caggccagtt gtgatgataa tttttttcac cacaataatt ttacgggtaa cagttttgac
     6001 ataggcacca acggaacgct ggtgctcaac aaatttgaaa aaaattactg ggacaagtat
     6061 gaaggatatg atatgaataa ggacggaatt ggtgatattc cctatcaccc ggtaagtatg
     6121 tattccatga tcgtcgaaga aaatcccacc acactaatac tgtttcgcag ttttatggtc
     6181 agccttcttg acaaagccga aaaagccatt cccagcctga cccctgaaaa tttatacgac
     6241 aatcaaccaa tgatgaaaat gctttcctta tgatccacat tgaagctgta tacaaaaaat
     6301 tccgccggct gcaggtattg gaaaatatat cggctcgttt tacagccgga caggtgatcg
     6361 ccctgatcgg acccaacggt tcgggtaaga ccaccctgat taaatccatc ctgggtatgg
     6421 tgcggcccga taaaggaacc attcgggtaa aagaccattt ggtaacgggc aatccggatt
     6481 atcgtcagtt tatcggatat atgcctcaga taggccgcta cccggacaat atgaaagtgg
     6541 gtcagctctt tgaaatgatg aaaaaaatcc gcggtataga atccgtggat atcgatctgc
     6601 tcacccgctt tgagatcgaa aagatctatg aaaaacccat gcgcaccctc agtgggggca
     6661 cccgccagaa agtgagtgcg gcactggcct ttctctttga tccccccatc ctgatcctgg
     6721 atgaacccac ggccggtctt gacccgcttt cttccgaaat cctgaaagaa aaaatcattc
     6781 aggaaaaaaa gaaaaacaaa ctcattctga ttacttcgca cgtactaagt gacctggaag
     6841 aactcaccag ccatatcatg tacctgcagg aaggaaaact gcagtttatc aaagacctgg
     6901 aaacgctgca ggaagaaacc ggggagatta aattaagcaa ggccattgcc aaactgatgc
     6961 gtggccagga aacgagcagt gcctggatcg accagttgtt tgctgttgaa cagaataaag
     7021 agaaccctca ataaactgaa tattatgctg aagttatcca gatacgtatt atatgatatt
     7081 ctacggaata aaattatcat cgcttatacc ctgtttctgt tgctggtctc tttaagtatg
     7141 ttccgcatgg aagaaaatgg cagcaaggcc atgctcagtt tactcaatat tgtattgatc
     7201 gttttgcccc tggtgagcct ggtattcacc actatccact attataattc gtatgaattt
     7261 attgagctga tgctttcaca acccctgagc cg
//
LOCUS       DAOPMW010000141         7286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11568_length_7286_cov_8.580141, whole genome shotgun sequence.
ACCESSION   DAOPMW010000141 DAOPMW010000000
VERSION     DAOPMW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11568_length_7286_cov_8.580141"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..500
                     /gene="mraZ"
                     /locus_tag="PLU11_08060"
     CDS             33..500
                     /gene="mraZ"
                     /locus_tag="PLU11_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPN59111.1"
                     /translation="MTGFLGEFEATVDAKGRFLLPAGFKRQLPEEETNRFVINRGFEK
                     CLALYPMRTWEPLFEKITGLNEFDPKQREFRRAFLNGATFVEPDTAGRILLPPNLKAY
                     AGLQKDIVLMATGDKLEIWDSNKYKEIFESLSSDTFSDLGDQVLGSGSDKSKS"
     gene            512..1465
                     /gene="rsmH"
                     /locus_tag="PLU11_08065"
     CDS             512..1465
                     /gene="rsmH"
                     /locus_tag="PLU11_08065"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPN59112.1"
                     /translation="MEQPNQQKGASGLPADSQDYHIPVLLSETLEGLNIQPDGIYVDC
                     TFGGGGHSRAILSRLGAKGKLVAFDQDDDAKKNLPDDERLVFVSHNFRHLQRFLRLEQ
                     ITAVDGIMADLGVSSHQFDEADRGFSTRFNADLDMRMDQRQALTAFDVVNTYTEQQLH
                     KLFEQYGEVTNSKTLARKIVQVRNTASLKTIDGFKNAVRDIVKGNPNKYFAQVFQALR
                     IEVNDELGALKEMLEQIPNLLKPGGRVAIITFHSLEDRLVKNFFRRGSFDEPEENPFI
                     NTEPVNELKIITKKPIGPTEEEMKRNPRSRSSKLRVAEKKG"
     gene            1601..1957
                     /locus_tag="PLU11_08070"
     CDS             1601..1957
                     /locus_tag="PLU11_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPN59113.1"
                     /translation="MPEQKTKEGELKAEQSNGLPRWKQWLNYQSIVKQVPFFLFLAVL
                     AVLYIYNGHQADKTIRSINRTEKEVKELQYEYTAVKGELISRSMQSKLMEAVKPLELK
                     ELTESPVVLTDSSLAK"
     gene            2029..4158
                     /locus_tag="PLU11_08075"
     CDS             2029..4158
                     /locus_tag="PLU11_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPN59114.1"
                     /translation="MEVKRDILWRVYVSFLGIVVLSLLVLGRAFYIQQVEGEHWRSKS
                     DSLHQRFIELDARRGTIYSEDGQMLSASIPTFDIYIDFDADGLREKKGKRFYDNIDSF
                     SLALAGYFGDKSSAQYKAELKAAFKKNVRYFPLKKKLSFEQYKTFREFPLARLGRNRS
                     GIIVETNSKRLPPFGLLASRTIGLSREHMASDGKVRKQNVGLEKSYDSVLSGKSGQKL
                     VRFIAGGTAIPIEGSETDPENGRDIYTTLDVQIQDITETALLRMMQQCEGPYGTAIVM
                     ETATGKIKAMANLGRRPDGSYWEDDNYALRTTEPGSTIKLVTLLSVLDKGTSKPNDLV
                     EVGTGRLQVGNKMITDAERQPRSVLTLKECFAHSSNIGMSQVAYKAFAQKPAELKEYM
                     HRFHLDTRVPVDLANLPKPSMAPLDQKGSAQGNMLWMSFGYGIQVSPLHTLMLYNAVA
                     NNGKMMKPYLVNSIRNNGVLYKQFEPTILTDNLCKPDVIKVARESMEMVVTEGTAKRV
                     LAGLPFAVAGKTGTAHVSDGPIKYDHGVYQASFVGYFPADRPQYTVIVVVRTKPHAAS
                     HFGGTVAAPVFREIATKLYAMYVDKKDPSQYAVKKDSAAYFYAGYTPDIKNVYDVLKV
                     KYADSASTGNWSMVYAANYQPVVKASSVKKKVMPNVRGMGLKDALYLLENMGVKVTVT
                     GKGKIAAQSVPPGTELAKGVTVLLELS"
     gene            4187..5650
                     /locus_tag="PLU11_08080"
     CDS             4187..5650
                     /locus_tag="PLU11_08080"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPN59115.1"
                     /translation="MELKDVLYKVPIRGVAGATSVIINDLQLDSRKVKPGAAFIAVKG
                     AAADGHLFIEKAIENGAAVVVCEEWPEQRNEAVIYVQVENSAAAAGYMACNFYGRPSE
                     KVKLVGVTGTNGKTTIATLLYKLFTRLGYKTGLLSTVENRIGEEIVPATHTTPDAISL
                     NYLLKRMAEEGCAYVFMETSSHAIHQHRVTGLTYAGGLFSNITHDHLDYHKTFDEYIR
                     VKKAFFDSLPAGAFAISNADDKRGMVMLQNTNAKKYYYSLKTLADFKGKILDNSLSGL
                     QMTVNDIEVHFRLIGEFNAYNLLAVYGAAVCMGEDKQEVLTALSELTGAEGRFDYLVS
                     VNDKVIAIVDYAHTPDALLNVLATIKKLRKGFEQIITVVGCGGDRDKTKRPVMATVAC
                     EHSDRVIFTSDNPRSEAPEQIIRDMEDGLAPAYRRKYISITDRREAIKTALSLAQKED
                     IVLVAGKGHEKYQEIKGVRSHFDDKEEVVEFFKLLNK"
     gene            5671..6948
                     /gene="mraY"
                     /locus_tag="PLU11_08085"
     CDS             5671..6948
                     /gene="mraY"
                     /locus_tag="PLU11_08085"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPN59116.1"
                     /translation="MLHHLFEWLNNQGIEIPGSRLFGFITFRVLAAVLLSLFITLVYG
                     KKLIRFLQKKQISESIREEGLAGEESKKGTPTMGGIIIIMAIIIPTLLLADLTNVYVQ
                     VMLISTVWLGIIGFIDDYLKLKSKRMAQQKGADYKKSNKDGLAGWFKILGQVGLGILV
                     GATLYFNSDVKIWREYVGKNPSADSTVKEKTYNGKTRYFVETKEPITTIPFVKNHEFN
                     YSKLLPASMRDYTWALYILIVIFIITAVSNGANLTDGLDGLATGTSALIGTLLGIFAY
                     ASGNLVFADYLNIMYIPNLGELSIFIAAMIGGCVGFLWYNSYPAQMFMGDTGSLTLGG
                     IIASLAIIVHKELLIPIFCGVFLVEALSVMIQVGYFKYTKKKYGEGRRIFLKAPLHHH
                     YQVKKYHEVKIVTRFWIVTVLLVVLSIVTLKLR"
BASE COUNT         2138 a         1603 c         1912 g         1633 t
ORIGIN      
        1 aattaattca aacttgattc aattaactga taatgacagg ctttctcgga gaattcgagg
       61 caacggtgga cgccaagggg cgtttcttgc tcccggcggg gttcaagagg cagttgccgg
      121 aagaggagac caaccggttc gttatcaaca gggggtttga aaaatgcctg gcgctctatc
      181 cgatgaggac ctgggagcca ctctttgaaa agattaccgg cctgaatgag tttgacccca
      241 agcaacggga gttcagacgg gcattcctga acggcgccac ttttgtggag cccgacaccg
      301 ccggtagaat attgttgcca ccaaacctga aggcatatgc cgggctgcag aaggatatcg
      361 tgctgatggc aacaggagac aaattagaaa tctgggatag taataagtac aaagagatct
      421 ttgaatcact ttcttcggac acattcagtg atttgggcga ccaggtgctg ggtagcggat
      481 cggataagtc gaaaagttaa aacggtaaag catggaacag cccaatcaac aaaaaggtgc
      541 atccggctta cccgctgact cacaagacta tcacattcct gttctgctca gcgaaaccct
      601 ggaaggcctg aatattcaac cggacggtat ttatgtggat tgcacatttg gcgggggtgg
      661 tcactcaaga gcgattctct ccaggctggg tgcaaaagga aaactggtcg cctttgacca
      721 ggatgacgat gcaaagaaaa acctgcccga tgacgaaaga ttagtatttg tttcacacaa
      781 ctttcgtcac ctgcagcgtt tcctccggtt agaacaaatc accgcagtgg atggtattat
      841 ggccgacctg ggcgtcagca gtcaccagtt tgatgaggca gacagagggt tctccacccg
      901 cttcaatgcc gatctggaca tgcggatgga tcagcgccag gcactgaccg cttttgatgt
      961 ggtgaatacc tatactgaac agcagttgca caaactgttt gagcagtatg gagaagtcac
     1021 caactccaaa acactggccc gtaaaattgt acaggtgagg aatactgctt cactgaaaac
     1081 aatcgacggt tttaagaatg cagtccggga tatagttaaa ggaaatccca acaagtactt
     1141 tgcccaggtt ttccaggctt tgcggataga agttaatgac gagttaggcg cattgaaaga
     1201 aatgcttgag caaatcccca acctgttaaa acccggaggc cgggtcgcca tcatcacgtt
     1261 tcattctctc gaggacagat tggtaaagaa ttttttccga cgcggctcat ttgacgagcc
     1321 cgaagagaac ccttttatca ataccgaacc ggtcaacgaa ctgaagatta tcacgaaaaa
     1381 accaatcggg ccaacagaag aagagatgaa acggaatccg cggtcaagaa gttccaaact
     1441 cagggtggcg gaaaagaaag ggtaagcaga tcatattaat aagggaacta cgaacacaag
     1501 atataagggt atacaaactt tggaaaatct atatcctttg aaatcaaccc ttgaaaccaa
     1561 gggcgatgaa aaaccagact aacacgcgaa aaacgcatgt atgccggaac aaaaaacaaa
     1621 agagggggag ttgaaagcgg aacagtccaa cgggctgcct cgctggaagc aatggctgaa
     1681 ttatcagtcc attgtaaaac aggtcccctt ctttttgttc ctggcagttc tcgcagtact
     1741 gtatatctac aacggtcatc aggccgataa aacgatacgc agtattaacc gcacggaaaa
     1801 agaagtgaag gagttacagt acgagtacac cgcggtaaaa ggagaactga tttcacggag
     1861 catgcagagt aaactgatgg aagcggtgaa gcccctggaa ctgaaagagc tgaccgaatc
     1921 cccggtggtg ctgacagaca gcagcctggc gaagtaacac aacaacggac ggacaactgc
     1981 gcaggcagtt tgcatataaa caactaagga cacaatcaat taagggcatt ggaagtaaaa
     2041 cgtgacatac tttggagagt atacgtcagt tttcttggca tcgtggtgct gagtttactg
     2101 gtactcggaa gagcctttta tatccagcag gtggagggcg aacactggcg cagtaaaagc
     2161 gacagtcttc atcagcgctt tatcgaactg gatgcaagaa gaggtaccat ctacagcgaa
     2221 gacgggcaga tgctcagcgc ttccatcccc acttttgata tttacatcga ttttgatgca
     2281 gacggactcc gggaaaagaa aggaaaacgt ttctatgata acattgactc gttctccctg
     2341 gcactggctg gttacttcgg ggataaatcc tccgcccagt acaaagccga actgaaagcc
     2401 gcgtttaaaa agaatgtccg ttattttccg ctgaagaaaa aactgagctt cgaacaatac
     2461 aaaaccttcc gtgaatttcc gctggcccgc ctgggaagaa acagaagcgg catcatcgtg
     2521 gagaccaaca gcaaacgcct gcctcctttt ggtctgctcg ccagccgtac cattggtctt
     2581 tcccgcgaac atatggccag tgatggaaaa gtccggaagc agaatgtagg cctggaaaaa
     2641 agttacgaca gtgtgctgag cggtaaaagc ggacagaaac tggtgcgctt tatagccggc
     2701 gggactgcca tcccgattga aggatcagaa acagatccgg aaaacggaag agatatctat
     2761 acgacgcttg atgtacagat ccaggatatt acggaaacgg ccctgctccg catgatgcag
     2821 cagtgtgaag gcccttatgg taccgcgatc gtgatggaaa ccgccaccgg aaaaatcaaa
     2881 gccatggcca acctgggacg tcgtccggat ggcagctatt gggaagatga taactacgcc
     2941 ctgcgtacaa ctgagcccgg atcgactatc aaactggtta ctttattatc cgtactggac
     3001 aaaggaacga gtaaacccaa tgatctggtg gaagtgggca ccggccgctt acaggtagga
     3061 aataaaatga ttaccgatgc ggaaagacaa cccaggagtg tactgaccct gaaagagtgt
     3121 ttcgcacaca gttccaatat tggtatgagc caggtggctt acaaggcatt cgcacaaaag
     3181 ccagctgaat taaaagaata catgcatcgt tttcacctcg atacccgggt gcctgttgac
     3241 ctggctaacc tgccaaagcc ctctatggcc ccgctggatc agaaaggaag cgcccaggga
     3301 aatatgctct ggatgagttt tggttatggc atccaggtga gtccgctcca taccctgatg
     3361 ctctacaatg cagttgccaa caatggtaaa atgatgaagc cttacctggt caacagtatc
     3421 cggaacaatg gagtgcttta caaacaattt gaacccacca tcctgacgga taatctctgc
     3481 aaaccggatg tgatcaaagt agcccgtgag tcgatggaaa tggtggtgac cgaaggaaca
     3541 gcgaagcggg ttttagccgg attacctttt gccgtggctg gtaaaacagg aacggcgcac
     3601 gtatccgatg gtccgatcaa atatgatcat ggtgtatacc aggccagctt tgtaggttat
     3661 ttcccggctg atcgtccgca atacacggtc attgtggtgg tacgcaccaa gccacatgcc
     3721 gcctcacact ttggaggtac cgtggccgcc ccggtgttcc gcgagattgc caccaagctg
     3781 tatgcgatgt acgtggataa gaaagaccct tcccagtatg cggtgaagaa agacagcgcg
     3841 gcttatttct acgccggcta tacaccggat atcaaaaatg tatacgatgt gctgaaagtg
     3901 aaatatgccg actctgcttc aaccggtaac tggtccatgg tgtatgccgc caactaccag
     3961 cccgtggtta aagccagttc ggtgaaaaag aaagtgatgc ccaatgtacg tggcatggga
     4021 ttaaaagatg ccttgtacct gctggagaat atgggtgtga aagtaacggt aacgggtaag
     4081 ggaaaaattg ccgcgcagtc cgtacctccg ggtaccgaac tggccaaagg ggtaaccgtg
     4141 ttgctggaat taagctgaga acagttaaac gattaatata acggacgtgg aattaaagga
     4201 tgttttatat aaagtgccga tccggggtgt agccggggcc acttccgtta tcatcaatga
     4261 cctgcagctg gattcaagaa aagtaaaacc cggagctgca tttatagcag taaaaggtgc
     4321 cgcagcagat ggtcacctgt tcattgaaaa agcgatagaa aacggggccg ccgtagtggt
     4381 ttgtgaagaa tggcccgaac aacggaatga agccgtgata tatgtgcagg tggagaacag
     4441 tgcggcggca gcgggctata tggcttgtaa tttttacggc cggccttcag agaaagtaaa
     4501 actggtgggt gtcaccggta ccaatggaaa aacgacgatc gcgactttat tatacaagct
     4561 ctttacaaga ttgggatata aaacaggatt gctcagcacc gtggaaaaca ggatagggga
     4621 agagatagta ccggccacgc ataccacacc ggatgcaatc agcctgaact atttgctgaa
     4681 gagaatggcg gaggaaggat gtgcgtatgt gtttatggaa accagctcac atgccattca
     4741 ccagcaccgg gtaaccggac tgacctatgc cggcggactg ttcagcaata tcacgcatga
     4801 tcacctggat taccacaaaa cgtttgatga gtatatccgg gtaaagaaag cctttttcga
     4861 ttcattgccg gccggcgcct tcgccatcag caatgcggat gataagagag gaatggtgat
     4921 gctgcagaac acgaacgcga agaagtatta ctacagcctg aaaacgctgg ccgactttaa
     4981 aggaaagatc ctggacaaca gcctgagcgg attacagatg acggtcaatg atatcgaagt
     5041 gcatttccgg ctgatcgggg agttcaatgc ctacaacctg ctggctgtat atggagcggc
     5101 ggtttgtatg ggtgaagaca aacaggaagt actgaccgca ttaagtgaac tgaccggggc
     5161 tgaaggaaga tttgattacc tggtttccgt taatgacaaa gtgatcgcca tcgtggatta
     5221 tgcacacacg ccggatgcct tgctgaatgt actggccacg attaagaaac tgagaaaagg
     5281 atttgaacag atcattaccg tagtaggatg cgggggcgac cgggataaaa caaaaaggcc
     5341 ggtgatggcg accgtagcct gcgaacacag cgaccgggtg atttttacca gtgataatcc
     5401 ccggagtgaa gcaccggagc agattatccg ggatatggaa gatggattag cgccagcgta
     5461 cagacgaaaa tatatctcaa tcaccgacag gcgggaagcc attaaaacgg cgctcagtct
     5521 ggcacaaaaa gaagacatcg tactggtagc aggcaaggga catgaaaaat accaggagat
     5581 aaaaggagtg cggagtcact tcgatgataa agaagaagtg gtggagtttt ttaaactgtt
     5641 gaataagtaa atcaataacc ggcaacgaac atgttacatc atttatttga atggctgaat
     5701 aaccagggga tcgagatccc cggcagccgg ctgtttgggt tcatcacctt ccgggtgctg
     5761 gctgccgtgc tgctttcctt attcatcacc ctcgtttatg gtaaaaaact gatccgcttc
     5821 cttcagaaaa agcaaatcag tgaaagtatc cgtgaagaag gactggccgg tgaggaaagc
     5881 aaaaaaggta cacccacgat gggcgggatc atcatcatca tggcgatcat catcccaacc
     5941 ctcttgctgg ccgatctgac gaatgtctat gtacaggtaa tgctgatcag taccgtatgg
     6001 ttggggatca tcggatttat tgatgattac ctgaaactga agagtaaaag aatggcgcag
     6061 cagaaaggcg ctgattataa aaaatcaaat aaagacggac tggccggctg gttcaagata
     6121 ctgggtcagg tgggactggg tatacttgta ggcgccaccc tttatttcaa tagtgatgtg
     6181 aaaatctggc gtgagtatgt tggaaaaaat ccgtctgctg attccacagt aaaggaaaaa
     6241 acatacaatg gcaagacccg ctactttgtt gagaccaaag agccgatcac tacgattccg
     6301 tttgtaaaga atcatgagtt taattactca aagctattac cagcttccat gcgtgattac
     6361 acttgggcat tgtatatcct gatcgtgatt tttatcatca ccgcggtatc caatggcgcc
     6421 aatctgacgg atggtttgga tggactggct acaggcacat cagccctgat tggcaccctg
     6481 cttggcatct tcgcttatgc gagtggtaac ctggtctttg cggattacct gaacatcatg
     6541 tatatcccga acctgggtga gctatccatc ttcatcgccg cgatgatcgg aggatgtgta
     6601 ggatttctct ggtacaattc ctacccggca cagatgttca tgggtgatac cggtagtctg
     6661 acgctcggtg gaatcattgc ttcactggcc attattgtac acaaggaatt gctgattccc
     6721 atcttctgtg gcgtgttcct ggtggaagcc ctgagtgtga tgatccaggt gggatatttc
     6781 aagtatacca agaaaaaata tggcgaaggc cgccggattt tcctgaaagc cccgctgcat
     6841 caccactacc aggtaaagaa ataccatgaa gtgaaaattg taacgaggtt ctggattgta
     6901 acggtattgc tggtggtact gagtattgta acgctgaaac tgagataaac cataccggtc
     6961 tgcagcgggg agcaggcggg tgaactatat aaccgaaaaa cccggaatcc aaccctgaag
     7021 aagacgatca ttccgatccg agcgaattgt ggtgcagttg tagaatatcc aatacttgtg
     7081 aagagccgtg ttccgtacac tgaattattg ttatgggaaa aagaatggtc atactgggtg
     7141 ggggagaaag cggcgtagga gctgccctcc ttgcaaagca aaaaggatat gacgttttcc
     7201 tgtccgatgg aagttctttg aagaaagagt atcgtaacga attaaccgaa gccgggattg
     7261 cttttgaaga agggcaacat accgaa
//
LOCUS       DAOPMW010000142         7223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11708_length_7223_cov_6.024693, whole genome shotgun sequence.
ACCESSION   DAOPMW010000142 DAOPMW010000000
VERSION     DAOPMW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11708_length_7223_cov_6.024693"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLU11_08090"
     CDS             <1..1190
                     /locus_tag="PLU11_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HPN59117.1"
                     /translation="FEADKKKTGKPDWDFLERAFQKLLMNFTWWVNQKDANGTDIFEG
                     GFLGLDNIGVFDRNHMPPGIKKLQQADATSWMAMYSINMLRMSLELAEHNKAYEESAA
                     KFFRHFLNIGWAMHHIGKKDISLWDDKDAFYYDAIQFENGSSERLRIRSLVGIIPMLA
                     VEVMHGGLFEQLKQFNTRLEVIRLTRPDLTRIISDINKKNADGNYLFAIMIGDRMEHL
                     LNRLLDEAEFLSDYGIRSLSKIHETPYTFSYQGNEYSIRYEPGESSSSMFGGNSNWRG
                     PIWLPLNYLIIHSLRKYYDYYGDSYTHEFPAGSGKKLNLKQIANELTKRLLKLFERNK
                     DRDGAFQYHDQQHTQWSENYFREHHLFYEFFHGDNGKGLGASHQTGWTALVVNLLLEI
                     EDD"
     gene            complement(1394..2080)
                     /locus_tag="PLU11_08095"
     CDS             complement(1394..2080)
                     /locus_tag="PLU11_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59118.1"
                     /translation="MAFRKKAFHVLEINPDILIVPECENPGKLIFPKNTKQPNSIFWA
                     GTNPHKGIGIFAYNNFSLKVDKSYKEDIKYFIPLKVTAPGIEFNLLAIWANNPKDPDG
                     RYVEQVWKGIQHYKKLIKRSNTILAGDFNSNKIWDADHRKWSHSSVVNELGKYGISSA
                     YHHHFNEYHGMEKGNTLYMYRHINKPYHIDYCFLSEDYIKKIKSVEIGEFDKWIKHSD
                     HLPLIVDINN"
     gene            complement(2198..3604)
                     /locus_tag="PLU11_08100"
     CDS             complement(2198..3604)
                     /locus_tag="PLU11_08100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN59119.1"
                     /translation="MNRKYIFLAFSVLFLFACRSTNEAGNLNQQMDEYLKGQADYFRF
                     NGNVLVAEKGKVIFQKSYGYADFDSKRLLNDSSVFELASISKQFTATAILLLKDKNKL
                     KLTDSLKQYFPELPYNGITLYHMLTHTSGLPDYEASMNSKWDRRKIAFNPDMIAFLAK
                     EKPPLVFAPGSRWEYSNTAYALLASIVEKISGQSFSTYLAKNIFQPLGMSHSRIYNTR
                     RSLKDTIPNYAYGYLYNDSLNKHEMPDSIPDLKFVIYLDGIQGDGVVNSTTADLFTWD
                     RAIKNHSLLLEATQNEMLKEQALADTVNKVYYGYGVFVEKTEAGKMISHSGGWPGYTT
                     FLARNVDKDQTFILLSNNSSNSPALTKALQQLANGQSVYIPYKHTEIVLDSTQLQKFT
                     GKYKTTTEFRIERTGNKIYRVFPNGQKTEIKPESPNKFFYADGTDRQIQFEMNEKQQV
                     VKGWLISYGVKTELQKLQ"
     gene            4079..5089
                     /locus_tag="PLU11_08105"
     CDS             4079..5089
                     /locus_tag="PLU11_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPN59120.1"
                     /translation="MKRIIFSTVAIFLLAGCKSELLNTIQISGAAQGTTYHITYLAGQ
                     QSNYREAIDSIFKKIDLSLSTYQPGSVISKMNRNDTAVLADEYFSDVFAKSMEVSEKT
                     KGLFDVTVAPVINAYGFGFTKKEKITQPLIDSLLRYIGYKKVRLADRKLVKELPQVML
                     DFNAIAQGYTVDVLSSFLESKGIRHYLVEVGGELRAKGKKGDDSDWIVGIEQPEENNA
                     DGASLNNLRVRIKDKSLATSGNYKKFYIENGKKYAHIINPFTGYPAMNNLLSATVIAS
                     DCMTADAYATAFMVMGLDKARQFLAENKNLGLEVLFIYDEKGISKTYMSKEFEANVDI
                     SH"
     gene            5190..5675
                     /locus_tag="PLU11_08110"
     CDS             5190..5675
                     /locus_tag="PLU11_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPN59121.1"
                     /translation="MNTPKLTAVQGYALVLLRILIGWHFLYEGIIKAYNPSWTSKGYL
                     LSASILKPFFAWLAGDSMISAIDYLNIIGLVAVGISLLIGIKVRWGCIAGMLLLLMYY
                     LAHPPFPGLPQGPAEGSYWVVNKNLIEMAALFVIYLFPLTTIFGLENLFARKKANTTI
                     N"
     gene            5681..>7223
                     /locus_tag="PLU11_08115"
     CDS             5681..>7223
                     /locus_tag="PLU11_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59122.1"
                     /translation="MANDTNNFKRWNRRDILKALGGIPFVGAVWYSGASLAGSAIREK
                     QFLLETLNIKASPPPASGPMSGKPLRIGIIGFGIRGEQLCRALGFASTGWLKDMSDNL
                     AKNPKDTRLTDFKAQENLNVKLAAICDVFDQRAENALKSFNSGDNKIKRFRTHTELIN
                     SGEVDAVIIATPDHWHAPISIEALNAGIHVYVEKPMTHNIAETYLLRDTVLKNPKTVF
                     AVGHQHRQTQSFLTAQDVISKNILGHVSLVQTNTNRNDDNGAWQYDIAESASPKTIDW
                     DLFLGNAPREPFNLEHFFRWRKWWAYGSGLSGDLMTHDYDRINCILKMGIPKTVTASG
                     GIYTHRDGRNVPDVMHVNMEFPDFSTGGSQVAGKEKGMTFVYSATLGNQFDRGTVLMG
                     HDGSMDLGNRITVYADPRSTKYAELIKEQRIEAGVPIYQYDPAVNGTDAVTSATAKYF
                     ANKGLLWTYRDGKRVDSTFLHLREWLSCIRNGGTPSCSIKEGFEEAISAHMGGLAYKL
                     GRRIEW"
BASE COUNT         2010 a         1461 c         1669 g         2083 t
ORIGIN      
        1 tgtttgaagc ggataaaaag aaaacgggta aacccgactg ggatttcctg gaacgtgctt
       61 ttcagaaact gctgatgaat tttacctggt gggtgaacca gaaggatgcc aatggcaccg
      121 atatcttcga aggaggtttc ctgggactgg ataatattgg cgtgtttgat cgtaaccata
      181 tgccgccggg gatcaagaaa ctgcagcagg ccgatgccac gagctggatg gccatgtatt
      241 cgatcaatat gctgcggatg tcgctcgaac tggcagagca taataaggcc tacgaggaat
      301 ccgccgccaa gttcttccgg cacttcctga atatcggctg ggccatgcac catatcggga
      361 agaaagatat ttcactctgg gatgataagg atgcttttta ttatgatgcc atccagtttg
      421 agaacggcag cagtgaacgc ctgcggatcc gttcgctggt gggtatcatt cccatgctgg
      481 cggtggaagt gatgcacggg gggctgtttg aacagctcaa acaattcaat acccggctgg
      541 aagtgatccg gctcacgcgg cccgatctca cccgtatcat ttccgatatc aataagaaga
      601 atgccgatgg taattatctg tttgccatta tgatcggcga ccggatggag cacctgctca
      661 accggctgct cgatgaagcg gagttcttat ccgattatgg tatccgttcc ttatctaaaa
      721 tacatgaaac accgtatacc ttcagttacc agggcaatga atacagtatc cggtacgaac
      781 ccggtgaaag ttccagttct atgtttggcg gcaattccaa ctggcgtggc ccaatctggc
      841 tgccgctgaa ttacctgatc attcactcac tccgcaaata ctacgactac tacggcgaca
      901 gctataccca tgaatttccg gccggttcgg gaaagaaact caacctgaaa caaatcgcca
      961 atgaacttac gaagcggttg ttaaaactat ttgaacgcaa taaggacaga gatggggcct
     1021 tccagtacca tgatcagcaa cacacacaat ggtcggaaaa ttatttccgg gaacatcatc
     1081 tcttttacga gtttttccat ggtgataatg gcaagggatt gggggcatcg catcagacag
     1141 gatggacagc ccttgttgtg aatctattgc ttgaaataga agatgattaa ttttagtagt
     1201 gcttgccctc cgaagctttt agcgaaggag gagtggtgaa tttattactg gagatggaag
     1261 atgagtgaga gaggaaaatc aatagccagc aggcaaaggc aggtcgatag tcaatagtaa
     1321 gcagtcggat tgtcagagat tgggaacata tacacattat atagctcttc ctcaattatt
     1381 ggaattcgat tatttagttg ttaatatcca caattaatgg caaatggtcg ctgtgtttta
     1441 tccatttatc aaactctccg atttctactg atttaatttt ttttatataa tcttccgaaa
     1501 gaaaacagta gtcaatgtgg taaggcttat taatatggcg atacatatat aaagtattcc
     1561 ctttttccat accgtgatat tcattgaaat gatgatggta tgcgctgcta attccatatt
     1621 tacccagctc gttaacaaca gacgaatgac tccattttcg atgatcggca tcccatattt
     1681 tgttgctgtt aaaatctcct gcaagaatgg tattgcttct cttaataagt ttcttatagt
     1741 gttgtatccc tttccaaacc tgttctacat aacggccatc aggatctttt ggattattag
     1801 cccaaatggc cagaagatta aattctattc caggagctgt tactttcaat ggaataaaat
     1861 attttatgtc ttctttatag cttttatcaa ctttcaaact aaaattattg taggcaaaga
     1921 tgccaattcc tttatgcgga ttggttccgg cccagaatat agaatttggt tgttttgtgt
     1981 tcttagggaa tatcagtttt cccgggttct cgcattccgg tacaattagt atgtccggat
     2041 ttatttctaa tacatggaaa gctttttttc taaaagccat gttacagttc catgttatta
     2101 ttttcatcgc gattaaattg aaatcttttt tgtaagactt atatgtcctg agaagaatgt
     2161 gttctgaaaa atcagctttc tttttagcct taaaattcta ttgcaatttt tgtaactcag
     2221 tttttacccc ataactaatc agccagcctt ttaccacctg ttgtttttca ttcatttcaa
     2281 attgaatctg cctgtcggtt ccgtcggcat aaaaaaattt gttgggggat tccggtttga
     2341 tttctgtttt ctgaccattt gggaaaacac ggtagatctt gttcccggtt cgttcaattc
     2401 tgaattcggt tgttgttttg tacttaccgg taaatttttg tagttgggtg gagtccaaca
     2461 ctatttcggt atgtttataa ggtatataca ccgattggcc atttgccagt tgttgtaatg
     2521 ccttggttaa tgccggagaa ttagaggaat tgttggacag taggataaag gtctggtctt
     2581 tatcgacatt tcttgcaaga aaggtggtat aacccggcca tcctccggaa tgtgaaatca
     2641 tctttcccgc ctcagttttt tcaacaaaaa caccatagcc gtagtagacc ttatttactg
     2701 tatcagccaa tgcctgttct ttcagcattt cattctgcgt tgcttcaagc aggagggaat
     2761 gatttttgat tgccctgtcc caggtgaata aatcggctgt agtggaattg acaacaccat
     2821 ctccctgaat gccatccagg taaatgacaa attttaaatc cggtatacta tccggcattt
     2881 catgtttatt taaactgtca ttatacaaat acccgtaagc atagttggga attgtatctt
     2941 ttagggaacg ccgggtatta tagatccggg aatgggacat acctaatggc tggaatatat
     3001 tttttgccag gtatgttgaa aatgactggc cggaaatttt ttcaacgatg gaagccagca
     3061 gtgcataggc agtattgctg tattcccaac gggagcctgg cgcaaacacc agcggtggtt
     3121 tttcctttgc caggaaagca atcatgtccg gattaaaggc aattttacgc ctgtcccatt
     3181 tactattcat cgacgcttcg taatccggaa gcccggatgt gtgtgtcagc atatgatata
     3241 atgtaatgcc attgtacggg agctccggaa agtattgctt cagtgaatcc gttagtttaa
     3301 gtttattctt gtctttcagt aataaaatag ctgtcgccgt aaattgttta ctaatagaag
     3361 ccagttcaaa tactgaggag tcattcagca atcgcttgct gtcaaaatca gcataaccgt
     3421 acgatttttg gaaaatcacc ttcccttttt cagcaaccag tacattccca ttaaaacgga
     3481 agtaatcagc ctgcccttta agatattcgt ccatctgctg atttaaattt ccggcttcat
     3541 tagttgaccg gcaggcaaac aggaacagca cggagaatgc caggaaaatg tattttctat
     3601 tcatatggaa ataagggtta aaatttctaa gaggattcgt aatagattga ccttcgtggt
     3661 ttagttatct gtagctgaaa atagatattt tcactgagaa acgttgtttc cacgggaata
     3721 cctgtttttt gggggttctg caccaatatc gggcaggggc gccggggcct gcctgcgggc
     3781 tccaaaagcg tgctgtttga ctgccggata ctattctcat tccccgcccg ggctgaaatc
     3841 ctttgcagta tcatatccac accctacttt tgttccgcca ggttttagta tcaccggatc
     3901 ctggaattta ccggaagtgg ttaggcaatc acgttagggt atttcaaaac ttacctatcc
     3961 atttctttga ggatagttct acttttaaag atagttctac tgtcgtttga attccagaat
     4021 acctgttgga atcagagtgg tcatttttcc gcaaatacta caacgaatgt ggacgataat
     4081 gaaacgtata atcttttcaa cggttgccat ttttctcctt gcaggctgca aaagcgagct
     4141 gttgaatacc attcagattt ccggagccgc ccaggggacc acttatcata taacctacct
     4201 ggcgggccag caatccaatt acagggaagc aattgattct atttttaaaa agatcgattt
     4261 gtctctttcc acttatcaac ccggttcggt tatttcgaag atgaacagaa atgataccgc
     4321 tgttctggcg gatgaatact tttcagatgt atttgctaaa tccatggagg tttcggaaaa
     4381 aacaaaaggc ctgtttgatg taactgtggc acctgttatt aatgcctatg gtttcgggtt
     4441 taccaaaaaa gaaaaaataa cgcaaccatt aattgatagc ctgctccggt atatcgggta
     4501 taaaaaggta cggttagccg atagaaaact ggtgaaagag ctgccccagg tcatgcttga
     4561 ttttaatgcc atcgcccagg gatatacggt ggatgtattg tcttcctttc tggaaagtaa
     4621 aggaatacgc cactacctgg tggaagtggg aggggaatta agggctaaag ggaagaaggg
     4681 ggacgacagt gactggatcg ttggaataga gcagcccgaa gaaaacaatg cagacggggc
     4741 atcattaaat aatttgcggg tcaggataaa agataagtca ttggccactt ccggaaacta
     4801 taaaaaattt tatatcgaaa acggaaagaa atatgcccat attattaatc cgtttaccgg
     4861 atacccggcc atgaataatt tgctcagcgc aaccgttatt gccagcgact gcatgaccgc
     4921 ggatgcttat gccacggcct ttatggtgat gggcttggat aaagccaggc agtttttagc
     4981 cgagaataaa aacctgggcc tcgaagtatt atttatttat gatgaaaagg gcatttcgaa
     5041 aacgtacatg tcaaaggagt ttgaagcgaa tgtggacata tcgcattaac atataatgag
     5101 ggggaggctg agcagttcag cgaatttaaa ggaaaagaga aaaatggcaa tccgatcagg
     5161 ttggaatatt aatataaaac tcaatcagta tgaacacacc gaaattaaca gctgtacagg
     5221 gttacgctct ggtactgctc cggattttaa ttggctggca tttcctgtat gaagggataa
     5281 taaaggccta taatccatcc tggacttcga agggctattt actaagtgcc tccattttaa
     5341 aacccttttt tgcctggctc gccggtgatt ccatgatttc ggctattgac tacctgaata
     5401 ttatcggcct ggtagctgtg ggtatcagcc tgctcatagg gataaaagtg cgctggggtt
     5461 gtattgcggg aatgttgttg ttactgatgt attacctggc acatcctcct tttcccggat
     5521 tgccgcaagg ccctgcggaa ggaagttatt gggtagtgaa caaaaacctg attgagatgg
     5581 cagcactttt tgtcatctac ctgttccccc tcactaccat ttttggcctc gaaaatttat
     5641 ttgcacgtaa aaaggcaaac accacaataa actaatcaac atggcaaacg atactaataa
     5701 ttttaaaaga tggaaccgga gggatatttt aaaagccctt ggcggcattc cattcgtagg
     5761 tgctgtttgg tattctgggg ccagcctggc tggttctgca atacgggaaa agcagtttct
     5821 gctggaaacg ttgaatataa aagcatcgcc tccccccgct tcaggtccta tgagtggcaa
     5881 gccactccgg attggcatca tagggtttgg tattcggggc gaacagcttt gccgtgcatt
     5941 aggttttgct tcgacaggat ggctgaaaga tatgagtgac aacctcgcga aaaatccaaa
     6001 agatacaagg cttaccgatt tcaaagcaca ggaaaacctg aatgtaaaac tggctgctat
     6061 ctgtgatgta tttgaccagc gtgccgaaaa cgcgttgaaa tcatttaaca gcggggataa
     6121 taagatcaaa cgctttcgta cgcatactga actgatcaat agcggggaag tggatgcggt
     6181 tattatcgcc accccggacc attggcacgc gccgatttca atcgaggcgc tgaatgcggg
     6241 gatacatgta tatgttgaaa agccaatgac ccataatatc gccgagacct accttttacg
     6301 ggatacggtt ttgaaaaacc ccaaaaccgt ttttgccgta gggcaccagc accggcaaac
     6361 acagagtttc ctgacagccc aggatgtgat cagcaagaat attctcggcc atgtgtccct
     6421 ggttcaaact aatacaaacc ggaatgatga taatggtgcc tggcaatatg atatagctga
     6481 gtcggccagt cccaaaacca ttgactggga tttgttcctt gggaatgcac cccgtgaacc
     6541 ttttaatctc gagcattttt tccgttggcg taaatggtgg gcttatggtt ctggattgtc
     6601 aggggattta atgacacacg attatgaccg gattaactgt attctgaaaa tgggaattcc
     6661 gaaaacggtt actgcttcag gcggtatcta tactcaccgc gacgggcgaa acgtaccgga
     6721 tgtaatgcat gtaaatatgg agttcccgga tttcagtacg ggcggaagcc aggtagccgg
     6781 aaaagaaaaa ggaatgacct tcgtatacag tgccacttta gggaatcagt ttgacagggg
     6841 taccgtgctg atggggcacg atggcagtat ggatctggga aaccggatta ccgtctatgc
     6901 tgatccaaga tcaacaaaat atgctgaatt gattaaagag cagcgtattg aagcgggcgt
     6961 tccgatttat cagtatgacc cggcagtaaa cggaaccgat gcggttacct cagccacggc
     7021 aaaatatttt gccaataaag gattgttgtg gacctaccgg gatggcaaac gggttgattc
     7081 cacctttctt catttaaggg aatggctgag ttgtatccgg aatggaggca cccccagttg
     7141 ttccatcaaa gaagggtttg aagaagctat ttcggcgcat atgggagggc tggcgtataa
     7201 attgggaaga agaattgaat ggg
//
LOCUS       DAOPMW010000143         7197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11765_length_7197_cov_8.169700, whole genome shotgun sequence.
ACCESSION   DAOPMW010000143 DAOPMW010000000
VERSION     DAOPMW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_7197_cov_8.169700"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2507
                     /gene="smc"
                     /locus_tag="PLU11_08120"
     CDS             <1..2507
                     /gene="smc"
                     /locus_tag="PLU11_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPN59123.1"
                     /translation="QVTDEEKSLEMLSEQLTQLNNEVSAKREILDERRRMLDSLRSEY
                     QQIQRSQFDAEKKVAVADTSVQNLQRVIHQIEEERVQREDQRLHLDDERQLKEKELGI
                     KKVDLEQLQQHQENTKEQILQTQAVLDSLRNNLADETRKLDSKKNEHDLLKSMIDSME
                     GYPDSVKFLHNNSNWNHSAPILSDVLYVNEEFRTALENVLEPYLSYYVVNNLQEGLQA
                     VHLLDENKKGKANFFLLDKVNDGASGIAAHQLEGAIPALSVVEVEEKYRKLAEHLLGN
                     VFIADSEDALQNSNGFVVIEKHGKYVKGKYSLTGGSVGLIEGKKIGRAKNLEKLQEEI
                     MAQDTVVESLRATIQARHNEVIAFNEDLRESAIRETEREIQQLTNAVFALHNKLENLH
                     SNQVSSEHRLEELNSQMQQTHTSVAGVRNELAEFNEMLLANSAKLAEADEAFKVTESD
                     YNEASRQYNEFNLNVTKQQSKISGLKQELEFKNNQLHDLRQQIESNTSHLADTRLNID
                     QSAGLLKEIEDALLELLRRKEEEERSLNEADQAYYNLRNQLSEKESEIRHQVKEKEQV
                     EYLLAAIKDKLNELKLQLAGMKERLNVEFRINLEDILGESRNGEVPVEELQEKADRMK
                     KRLENLGEVNPTAIEAFTEMKKRYDFIVEQKTDLVNAKDSLLKTIEEVEATANQKFLE
                     TFHKVRENFQKVFKTLFTEDDQADLVLENPENLADSGIDVVAKPKGKRPSSLTQLSGG
                     ERTLTATALLFAIYLIKPAPFCILDEVDAPLDDANVSKFTNMIRQFSDNSQFIIVTHN
                     KMTMSTVDVIYGVTMQEPGVSKLVPVDFRNLKTAQN"
     gene            complement(2763..3038)
                     /locus_tag="PLU11_08125"
     CDS             complement(2763..3038)
                     /locus_tag="PLU11_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59124.1"
                     /translation="MSGPHSNSHAEKYFSHILIGFASLIAFVFTLFYALIELKVTKFW
                     YFWAAAAAFLLVAGIYFCMSAFVHKVKSDFNRRAKQREQQKTFTADN"
     gene            3165..3749
                     /locus_tag="PLU11_08130"
     CDS             3165..3749
                     /locus_tag="PLU11_08130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HPN59125.1"
                     /translation="MKKILLFLLVLFCLTEINAQEKPEGLFIGSKAPDFKAKDQDGND
                     VRLKDLLKKGKVVLVFYRGYWCPYCNRELSRLQDSLSQILEKGATVVAVSPEKPEHIK
                     QTVEKTKAAYPVIYDEGMKIMKGYDVEYELEETTLARYRSSGLDIEKNNGTNGKFLPV
                     PAVYIIDKESNVTYRFFDADYKKRPSIAEIIRNL"
     gene            complement(3744..4412)
                     /locus_tag="PLU11_08135"
     CDS             complement(3744..4412)
                     /locus_tag="PLU11_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN59126.1"
                     /translation="MFKKFLEQLRWKRLARQLRKPEGPAGLEVARRMNTANEFLYDFT
                     LDEMLVGNNQSILEIGFGNGKFFYKQFNRAEELRITGIDFSEDMIREAKVINQDYLEQ
                     GKLLLVKGQSDKMPFQNASFDIIFCINVAYFWKEPQDHLVEIHRVLKPGGRLYTTIRT
                     PESLAAMPFSQYGFRTYTEEEWKLLCHLNNLAFIRSSHVHEPAESPAGLIRSVCLVAE
                     KKHL"
     gene            complement(4434..5459)
                     /locus_tag="PLU11_08140"
     CDS             complement(4434..5459)
                     /locus_tag="PLU11_08140"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPN59127.1"
                     /translation="MKGIRLFFILGLLTPLFLFAQQPKLEMLTSGTATSIRGLSVVND
                     QVIWVSGSKGTIGRSIDGGQTWKWSVVTGFEKNDFRDIEAFDANTAIIMSVDAPAYLL
                     KTTNGGDTWKIVYENKTKGMFLDAMEFWNDKAGIVIGDPIDGKFFVARTFDGGISWQE
                     IPVDKRPQADSGEACFASSGTNIKVLDRDEAVFVSGGLRSRLFTRNPPVALPLLQGTE
                     STGANSVAVWNNRKMQGGRKLIVVGGDFTQPASMDKNCFYSNDRGKTWTAPVTPPHGY
                     RSCVEYFSMQKAVSCGLNGIDITTDGGRNWTWISQEGFHVCRKAKNGTLIFFAGNNGK
                     IGKIALD"
     gene            complement(5466..6161)
                     /locus_tag="PLU11_08145"
     CDS             complement(5466..6161)
                     /locus_tag="PLU11_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPN59128.1"
                     /translation="MRNVLFSLLLLAVVSCQSQSGGNDIHFGPEAFEKGLQSESAQVL
                     DVRKATEFKNGHLKNALQADWTNKQQFFERIKYVDKDRPVYIYCLAGGRSAAAAAWMR
                     ENGFTKVVELSGGINAWKNAGKPVEGNTNVKQMTPEEFALSIPADKTVLVDFGAEWCP
                     PCVKMEPVLAALRQDASLKFEFIKIDAGIHTDLMNVQQIEAIPVFIVFKNGKETWRKQ
                     GLVSQEELTAQLK"
     gene            6278..6685
                     /locus_tag="PLU11_08150"
     CDS             6278..6685
                     /locus_tag="PLU11_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59129.1"
                     /translation="MKRRIVVFVMMVFVAAACSKELSYEGPDSPAEGSMQSALTGDCL
                     PKTVSGTYQVAVPLVADSNTISVAVNVTTTGTYLVTTDTVNGFFFNSSGRFTSPGTNT
                     VKLRSTGTPFAAGVNNFRISFDSTFCDVQVTVQ"
     gene            complement(6702..>7197)
                     /locus_tag="PLU11_08155"
     CDS             complement(6702..>7197)
                     /locus_tag="PLU11_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPN59130.1"
                     /translation="KYSAEERAIYQLVYDAQEAAFKTLKNGSKFADASNAARETIAEG
                     LVKLGILKDKKDVWKFYPHGLGHHIGLDVHDRGAMETLLKDMVITIEPGIYIQSNSDC
                     DKKWWGIAVRIEDDLLITETGYELLSRFSPRKIEEVEKLISQKSALDQFKLDPLESGK
                     KKGF"
BASE COUNT         2055 a         1610 c         1688 g         1844 t
ORIGIN      
        1 tgcaggtaac ggatgaggaa aaaagtctgg aaatgctgag cgagcagctg acccagctga
       61 ataatgaagt aagcgccaaa cgggaaatcc tggatgaaag acgccggatg ctggatagtc
      121 tccgtagtga ataccagcag atccagcgca gccagtttga tgccgaaaag aaagtggccg
      181 tggcggatac ttctgtacaa aacctgcaac gggtcatcca ccagatcgaa gaagaaagag
      241 tacaacggga agatcagcga ctgcacctgg atgatgaacg ccagctgaaa gaaaaggaac
      301 tgggtatcaa aaaagtggac ctggaacaat tacaacagca ccaggaaaat accaaagaac
      361 agattttaca aacacaggct gtattggata gcctgcgtaa taacctggcc gatgaaaccc
      421 ggaaactgga ttccaaaaag aatgaacatg acctgttgaa gagcatgatc gactccatgg
      481 aaggctatcc ggattccgtg aaattcctgc ataataacag taactggaat cacagcgccc
      541 cgattctttc cgatgtgttg tatgtaaatg aagaattccg caccgcactg gaaaatgtac
      601 ttgaacctta cctgagttac tatgtggtga ataacctcca ggaaggactt caggcggtac
      661 acctgctgga cgagaataaa aaaggcaagg ccaatttttt cctgctggat aaagttaatg
      721 atggtgccag cgggattgct gctcaccaac tggaaggcgc gattcctgca ttgagtgtgg
      781 tggaagtgga agagaaatac cggaagctgg ccgaacacct cctgggtaat gtatttatcg
      841 ccgacagtga agatgcttta cagaacagca atgggtttgt cgttatagag aaacatggta
      901 aatatgtaaa agggaaatac tccctgaccg gcggcagcgt aggtctgatt gaaggcaaaa
      961 agatcggtcg tgccaagaac ctggagaaac tgcaggaaga aataatggcc caggatactg
     1021 tggtggaaag tctccgtgcc accatccagg cccgtcacaa tgaagtgatc gcattcaatg
     1081 aagatctccg tgaatcagcc atccgggaaa cagagcggga aattcaacaa ttaaccaatg
     1141 ccgtttttgc cctgcataat aaactggaaa atctgcacag caaccaggtc agcagcgaac
     1201 atcgcctgga agaactgaac agccagatgc aacagaccca tacttcggtg gcgggtgtac
     1261 ggaatgagct ggctgagttt aatgaaatgc tgctcgccaa ttcagctaaa ctggccgaag
     1321 cggatgaagc gtttaaagta accgaatcgg attacaatga agcttcccgc cagtacaatg
     1381 aatttaacct gaatgttacc aaacaacaaa gcaagatcag cggactgaaa caggaactgg
     1441 aattcaaaaa taaccagctc catgatctcc gtcagcaaat tgaaagcaat accagtcacc
     1501 tggccgatac ccggctgaat attgatcaat ctgccggttt gctcaaagaa attgaagatg
     1561 ccttactgga actcctgcgt cgcaaagaag aagaggagcg gagtctgaat gaagcggacc
     1621 aggcctatta taatctccgg aaccagttga gtgaaaagga aagtgagatc cggcaccagg
     1681 tgaaggaaaa agagcaggtg gaatacctgt tggccgctat caaggataaa ctgaatgaac
     1741 tgaaactgca actggctgga atgaaggaac gactcaatgt ggagttccgc attaacctgg
     1801 aagatatact gggtgaatcc cggaatgggg aagtgcctgt ggaagaactg caggaaaaag
     1861 ccgaccggat gaaaaaacgg ctggaaaacc tgggtgaagt aaaccccacg gctattgagg
     1921 cattcacgga aatgaagaaa cggtatgact ttattgtaga gcagaagacg gacctggtca
     1981 atgccaagga cagtctgctg aaaacaatcg aagaagtgga agcaacggcc aaccagaaat
     2041 tcctggagac cttccataaa gtaagagaga atttccagaa agtattcaaa actctgttta
     2101 ccgaggatga ccaggcagac ctggtacttg aaaatccaga aaacctggcg gatagtggta
     2161 tcgatgtggt agctaaaccg aaaggcaagc gcccctcttc actgacccag ttgagcgggg
     2221 gagaacggac cctgacggct accgccctgc tgtttgccat ttacctgatc aaaccggccc
     2281 ctttctgtat cctggatgag gtggacgctc cgctggatga tgccaacgta agcaaattca
     2341 ccaatatgat ccggcagttc agtgataatt cccagttcat tattgtcacc cacaataaaa
     2401 tgaccatgag taccgtggat gttatttacg gggttaccat gcaggaaccg ggggtaagta
     2461 aactggtacc ggtggatttc aggaatctga agaccgcgca gaactagtta gtagctggta
     2521 gcctgcctgc cggcaaaggc aggtcggtag ttagtagtct gatgatgacg taaaaggatg
     2581 aatcaaagta atgtggtgat taattgcgat ccattagcat gattgaatga agaatcaata
     2641 ttgaatttaa aagagcccgt taaaacgggc ttttttttga ctcgaacatg attatgaatt
     2701 ttttactggt actatcattg aaagcaccag gcaatcttcc tgatgttcag taagcagccg
     2761 gcttaattgt ctgcggtaaa agttttttgc tgttcccgtt gtttggcccg gcggttgaaa
     2821 tcagatttta ctttatgaac aaaggcactc atgcagaaat aaataccagc taccaacagg
     2881 aatgcagccg ctgcagccca gaaataccag aatttggtaa cttttaattc aatgagtgcg
     2941 taaaagaggg tgaagacaaa agcaataaga ctggcaaaac cgatcagaat atgcgaaaag
     3001 tacttttcgg cgtgggaatt agaatgtgga cctgacatac atcgtaattt taaggaattc
     3061 aatggtctgc aaacttaata aaaataatct tctgttaatc tgttccggac agtaccgttt
     3121 tctgaacgga gtacttattt ttagtattta aaaggtttac ctgtatgaag aagatcctgc
     3181 tattcctgct tgttttgttt tgcttgaccg aaatcaatgc ccaggaaaaa ccggaaggtt
     3241 tgtttatcgg ttcaaaagca cccgatttta aagccaagga ccaggacggt aatgatgtaa
     3301 gattaaagga cctgcttaaa aaagggaaag tggtgctggt tttttacagg ggttactggt
     3361 gtccgtattg taaccgggaa ttatcacgtc ttcaggactc actctcacaa atacttgaaa
     3421 aaggagcaac cgtggtggca gtttcacctg aaaagccgga gcatatcaaa cagacggtgg
     3481 agaagaccaa ggctgcctat ccggtgatat atgatgaggg tatgaaaatt atgaaggggt
     3541 atgatgtgga gtatgaactg gaggaaacca cactggcccg ttaccggagc agtggactgg
     3601 atatcgaaaa aaataatgga accaacggga aatttcttcc ggtcccggct gtatatatca
     3661 tagataaaga atcgaatgta acctatcggt tttttgatgc ggattataaa aaaagaccca
     3721 gcatcgcgga gattatccgg aatttataag tgcttttttt ctgctacgag gcatacggaa
     3781 cggatcagtc ccgcagggga ttctgcaggt tcatgcacgt gggaagatcg tatgaaagcg
     3841 aggttattca ggtggcagag taatttccat tcttcttcgg tataagtcct gaaaccatat
     3901 tggctgaacg gcattgcggc caggctttcc ggggtccgga tagtggtata caaccggcca
     3961 cctggtttca acacccggtg aatttcgacc aggtgatcct gcggctcctt ccagaaataa
     4021 gccacattaa tacaaaaaat gatatcgaaa ctggcattct gaaatggcat tttatcgctt
     4081 tgccctttca ccaataataa tttgccctgc tccaggtaat cctggttgat aacttttgct
     4141 tcccggatca tgtcctctga aaaatcgatt ccggttatcc gcagttcttc cgcccggttg
     4201 aattgtttat agaaaaattt cccattccca aatccgattt cgaggatgga ctggttatta
     4261 ccgaccagca tttcatcaag tgtaaaatca tacaggaatt cattcgccgt attcattcgg
     4321 cgggccacct ctaatccggc tggtccttcc ggtttgcgaa gctgacgggc aagccgtttc
     4381 cagcggagct gttctaggaa ttttttaaac acaggttaag gtttgcaatg aaattagtcc
     4441 aacgcaatct taccgatttt tccgttattt ccggcaaaaa agatcaacgt gccatttttg
     4501 gctttacggc aaacatgaaa tccttcctgg ctgatccagg tccagtttcg tccgccgtct
     4561 gtagtaatgt caattccatt taacccgcag ctgactgctt tttgcatcga aaaatactcc
     4621 acacaactgc ggtaaccatg gggtggagta accggtgcgg tccaggtttt gccccggtca
     4681 ttgctgtaaa aacagttttt atccattgaa gcaggttggg taaagtcgcc accaactaca
     4741 atcagtttgc gcccaccctg cattttacgg ttattccata cggctacaga attggccccg
     4801 gtggattcag taccctgcag caggggaagt gctaccggtg gattacgagt aaataaacgg
     4861 gagcgaagtc cgccgctgac aaaaaccgct tcatcgcggt ccagtacttt tatgttggtg
     4921 ccactggaag caaaacaagc ttcgccactg tcagcctgtg gacgtttatc caccgggatt
     4981 tcctgccagc tgatgccacc gtcaaaggtc cgggctacaa aaaattttcc atctataggg
     5041 tcgccgatta caatgccggc cttatcattc cagaattcca tggcatccaa aaacatgccc
     5101 tttgttttat tttcataaac aattttccag gtatcacccc cattggtggt cttcaaaaga
     5161 taggccggcg catccacact catgatgatc gccgtattgg cgtcaaacgc ttcaatatcc
     5221 cggaaatcat ttttttcaaa cccggtgacc acggaccatt tccaggtttg tccgccatcg
     5281 atagagcggc caatggttcc tttactgcca ctgacccaga tgacctgatc attcaccacg
     5341 ctcagtccgc gtatactggt agccgttcca ctggtcagca tttccagttt tggttgctgg
     5401 gcaaacagaa aaagcggggt caataaaccc agtataaaaa acagacggat tcctttcatg
     5461 taagtttatt ttagctgggc ggtcagttct tcctggctca ccagtccttg tttacgccag
     5521 gtttctttcc catttttaaa tacgataaat acgggtattg cttcgatttg ctgcacattc
     5581 atcagatccg tatgaatccc ggcatctatt ttgataaatt caaatttcag gctggcatcc
     5641 tgcctgagtg cagccagtac gggttccatc ttcacacagg gcgggcacca ttcggccccg
     5701 aaatccacta acaccgtttt atcagccgga atagataagg caaattcctc cggagtcatc
     5761 tgtttcacat tcgtgtttcc ttccacgggc ttcccggcat ttttccaggc attgatcccg
     5821 ccggaaagtt ccaccacttt agtaaacccg ttttcccgca tccaggcagc ggctgcagca
     5881 ctacggccac cggcgagaca gtagatataa acgggccggt ctttatccac atattttatc
     5941 cgctcaaaaa attgctgttt atttgtccag tcagcctgaa gggcgttttt caaatgcccg
     6001 tttttaaact cagtggcttt tctaacatcc aacacttgtg cggattctga ttgcaatcct
     6061 ttttcaaagg cttcgggtcc gaaatggatg tcgtttcctc cagactgtga ttgacaggaa
     6121 acaacagcta ataataataa actgaataat acgtttctca tccgggattt ttttttcaaa
     6181 tgtagcataa tatttgccct gcgggagagg attcagtgct gacagggatc cattgataaa
     6241 attgtcttat ttttagcgtt taaagctgac tcggaatatg aaaagaagaa ttgttgtttt
     6301 tgtgatgatg gtttttgtgg ctgcggcctg tagtaaagag ctgagttatg aaggaccaga
     6361 cagtccggct gaaggatcca tgcaaagtgc gctcaccggt gattgcctgc ccaaaacagt
     6421 cagcggtacc taccaggtgg cggtacccct ggttgcggat tctaatacca tcagtgtggc
     6481 agtaaatgta acgacaacgg gtacctatct ggtcaccaca gatacggtta acgggttttt
     6541 cttcaattcc tcaggaaggt ttacttcccc gggaaccaat accgtcaaac taagaagtac
     6601 tggtaccccc tttgccgccg gcgtcaataa tttccggatc agttttgaca gtactttttg
     6661 tgatgtgcag gtgactgttc agtaattcat aggcaacgtg actaaaatcc tttctttttt
     6721 ccggattcca gcggatccag tttgaactga tccaaagcac ttttctggct gatgagtttt
     6781 tcaacttcct ctattttccg gggagaaaac ctggatagaa gttcatatcc tgtttcagta
     6841 atcagcaggt catcttcgat acgtacggca atgccccacc attttttatc gcaatcacta
     6901 ttggattgaa tatatatgcc cggttctatg gtgatcacca tatccttgag cagggtttcc
     6961 atcgcacctc tgtcatgtac atccagaccg atatgatgac ccaacccatg cggataaaat
     7021 ttccagacat ctttcttgtc tttaagaata cccagcttaa ccagtccctc agctatagtt
     7081 tccctggctg cattactggc atcggcaaat tttgaaccat tcttcagtgt tttgaaggcg
     7141 gcttcctggg catcatatac cagctggtat atagctcttt cctctgcgga atattta
//
LOCUS       DAOPMW010000144         7155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11859_length_7155_cov_7.948028, whole genome shotgun sequence.
ACCESSION   DAOPMW010000144 DAOPMW010000000
VERSION     DAOPMW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11859_length_7155_cov_7.948028"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLU11_08160"
     CDS             <1..450
                     /locus_tag="PLU11_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59131.1"
                     /translation="EIKKLYPDIKVIMLSFLNDHSVISKMMELGANSYITKESGAELI
                     YEAIRGVFENDFHFNDLTNKALLSGLRTKRNHESSMPQDVQLTEKEITILKLMCDEKS
                     TKEIADIVDLSPRTVEAIRDKLKTKTGTKSMAGLVMYAVKAGLVEQA"
     gene            668..1504
                     /locus_tag="PLU11_08165"
     CDS             668..1504
                     /locus_tag="PLU11_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPN59132.1"
                     /translation="MYISFNGKITPADQPVLLAANRGYRYGDGLFETMKLDNSVILLE
                     ELHFQRLIKGADLLKFELPGLFSRAKLVSAIQELAMKNGVNKLGRVRLSLFRGNGGLY
                     DADRTVQYLIECWPLSDRFSRLNENGLEIGIYPDARKQVDFFSGLKSANFLPYTMASL
                     YAKENKLNDCLLLNQDGHIADSTIANLFIVKDGQFLTPGPEQGAVDGVMRKYLVREML
                     AAGYKVTETPVTISALEAADEVFLTNAIHGIKWVRRFGDCMYSNRYSSEIYSRIIQTI
                     PR"
     gene            1520..2824
                     /locus_tag="PLU11_08170"
     CDS             1520..2824
                     /locus_tag="PLU11_08170"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPN59133.1"
                     /translation="MKPAVSVFWFRRDLRLTDNAGLFQALQSGQPVLPVFIFDTDILD
                     SLEDKDDRRVEFIHRALSGMQEELTALGSTLDVRYGKPLQVYQELFREYNVTSVYTNH
                     DYEPYALERDAAIGQWLKAKGCSFQTFKDQVILEKNEVLKDDGKPYTVFTPYSRKWKA
                     VLGAQTMPVFNTKKYFKHFFQQPAKAIPSLGSMGFTATGLPFPANSWKKEVISRYKEQ
                     RDIPSLPGTSRLSVHLRFGTISIRELAREAGVLNETFLNELIWRDFYHMILWHFPGVV
                     GHSFKPEYDKIRWRNNEHEFDAWCKGQTGYPIVDAGMRELNATGFMHNRVRMIVASFL
                     TKHLLIDWRWGEGYFARKLLDFDLAANNGGWQWAAGSGCDAAPYFRVFNPYLQTQKFD
                     PELKYIRQWVPEFEELTYARPIVVHEVARKRCLETYAAALKK"
     gene            complement(2813..3643)
                     /locus_tag="PLU11_08175"
     CDS             complement(2813..3643)
                     /locus_tag="PLU11_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPN59134.1"
                     /translation="MKLTLGFSPCPNDTFIFDALVNQKIDTGGLSFDVILEDVETLNQ
                     WALQGKLDITKLSFPAFFKSTPHYVLLDSGSALGKGVGPLLVSTTENTNPDVSKSRIA
                     IPGINTTANLLLSYAYPEARNKTAFIFSAIEEAVVSGETDLGLIIHENRFTYQDKGLK
                     KVMDLGEYWENKMKVPIPLGGIAAKRTLGTPLAKQVSTLIKESLAYAFTGYPEVTGYV
                     KDHSQTMSEQVMRQHIDLYVNNYSLQLGDDGKKAILELFNVYHSMQGNEKIITPDLFL
                     "
     gene            complement(3640..4281)
                     /locus_tag="PLU11_08180"
     CDS             complement(3640..4281)
                     /locus_tag="PLU11_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HPN59135.1"
                     /translation="MNILVIAATPIEINPFLEQLRNGLISRQPDVLITGIGLTATTWS
                     LTRQILIKKPDLVIQAGVGGCFDRRIPTGTVLAIKQEAIADQSVIELNTLRTLFDLKL
                     VPANQYPFSKGWLVNKNEVLKKVKLKKVNGISVNEITTDRKKVKFYEQQFAPVVESME
                     GAALHYVCLMEKLPFIQLRAVSNYIAERNKKNWNMKTSIINLNTELIRLLDKL"
     gene            4425..4853
                     /locus_tag="PLU11_08185"
     CDS             4425..4853
                     /locus_tag="PLU11_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPN59136.1"
                     /translation="MVYLTRIEHFNAAHKLYNPRWTKEKNEAVFGRCANENWHGHNFE
                     LYVTIKGYPDPDTGFIFDAKQLGKLVQERIIEQLDHRNLNEDVGFMKGKMCSIENLVI
                     GIWNELQPLLPADAPLHCLKLVETDKIYVEYYGEQEAVNK"
     gene            4909..5322
                     /locus_tag="PLU11_08190"
     CDS             4909..5322
                     /locus_tag="PLU11_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPN59137.1"
                     /translation="MSNKVAVFRKESFNAAHRLFNPGWDQATNDRVFGKCAFPNYHGH
                     NYDLVVKVVGVPDPATGYVMDMKILSDLVQREIVERFDHRNLNLDTVEFRNLNPTTEN
                     IAVVIYNLLRPHIGPELELQVRLYETPRNFVEYPV"
     gene            5334..5930
                     /gene="folE"
                     /locus_tag="PLU11_08195"
     CDS             5334..5930
                     /gene="folE"
                     /locus_tag="PLU11_08195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPN59138.1"
                     /translation="MAYSKIEQYDESTTSGLIKSYKDVLGLIGEDAEREGLQKTPERV
                     AKAMQYLTQGYQLDAHAILNSAKFHEEVSEMIVVKDIELFSMCEHHMLPFFGKAHVAY
                     IPNGWITGLSKVARVVDVYSRRLQVQERLTIQIKDAIKETLNPLGVAVVIEAKHLCMM
                     MRGVQKQNSVTTTSAFDGEFHKNSTRSEFMKLIGSRLS"
     gene            6017..6907
                     /gene="fabD"
                     /locus_tag="PLU11_08200"
     CDS             6017..6907
                     /gene="fabD"
                     /locus_tag="PLU11_08200"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPN59139.1"
                     /translation="MKHAFIFPGQGSQFPGMGKDHYENSAFAKKLFEQANEILGFRIS
                     DTMFGGTEEDLKQTNVTQPAIFLHSVIAFKSIESARPDMVAGHSLGEFTALVANGTLS
                     FEDALQLVAVRATAMQKACEVNPSTMAAVLNLADEKVEGICAEVQQETGEIVVPANYN
                     CPGQLVISGSRKGIDIACERMKAAGAKRALVLPVGGAFHSPLMLPAKEELQAKILSTK
                     FHSPTCAIYQNVAAKAVMDNEEIKQNLVDQLTGAVKWTQSVQAMIADGATRFTEAGPG
                     KVLQGLVLKINKEMQVEGVN"
BASE COUNT         1901 a         1582 c         1773 g         1899 t
ORIGIN      
        1 gagatcaaaa aattataccc cgatattaaa gtaatcatgc tctccttcct gaatgaccat
       61 tcggttattt ccaagatgat ggagctgggt gctaattcat atatcactaa agaatccggt
      121 gccgaactga tctatgaagc gatccgtggt gtatttgaaa atgatttcca tttcaatgac
      181 ctgaccaata aagccctgct gagtggtctg cgtaccaaaa gaaatcatga gtcatccatg
      241 ccacaggatg tgcaactgac cgaaaaggaa atcaccatcc ttaaactgat gtgtgatgag
      301 aaaagtacga aggaaattgc ggatatcgtg gacctgagtc cccggaccgt ggaagccatc
      361 cgtgataaac tcaaaaccaa aaccggtact aaatccatgg ccggcctggt catgtatgcg
      421 gtaaaagccg gacttgttga acaagcctga cttatctgag cacacaacga acaaatcctt
      481 attatttata aagaggctgt ctcaaaagag gcggcctctt ttttgtgact ggtgttctcc
      541 gctacgcgga gtttttttga cacatagaca cattgtaaca tagaatttgt atttctatgt
      601 atccctatgc ccctatgtgc ctatgtgtta aaaacgtcgc agccgtcttt tttcgttact
      661 ttgagccatg tacatttctt tcaatggaaa aatcaccccc gcggatcagc cggtattact
      721 ggctgccaac cggggatatc gctatggcga tggtttgttt gagacgatga agcttgataa
      781 cagcgtcatt cttctggagg aattgcattt ccagcgactg ataaagggcg ccgatctgtt
      841 gaagtttgag ttgcccggac ttttttccag ggcaaagctc gtttcagcca tacaggaact
      901 ggccatgaaa aacggtgtaa ataaactggg ccgggtccgc ttatcgcttt tccggggtaa
      961 tggcggtctc tatgatgcag accgtactgt acaatacctg atcgaatgct ggccactgtc
     1021 cgatagattc agccggctga atgaaaacgg gctggagatc ggtatttatc cggatgcacg
     1081 taaacaggtt gatttttttt cgggcttgaa gtcggctaat tttctacctt ataccatggc
     1141 ctccctttat gcgaaggaaa acaagctgaa tgattgcctg ctgctgaatc aggatggtca
     1201 tattgccgat agtacgattg ccaatttgtt tatagtaaaa gacggccaat ttcttacgcc
     1261 cggaccggaa caaggggcgg tggatggcgt gatgcggaaa tacctggtcc gcgaaatgct
     1321 ggcggccgga tataaagtaa ccgaaacacc ggtaacgatt tccgctttgg aagcagccga
     1381 tgaagttttt ctcaccaatg ccattcatgg tataaaatgg gtccgccgct tcggggattg
     1441 tatgtacagt aaccggtact cctccgaaat ttattcccgg atcatccaaa caattccccg
     1501 gtaaacttgt tgcacccaca tgaagcctgc tgtttctgtt ttctggttcc gccgcgatct
     1561 tcgtcttact gataatgccg gtttgttcca ggcgctacaa tccggacaac ctgtacttcc
     1621 cgtttttatt ttcgatacgg acatcctgga ttcgctggaa gataaggatg accgccgggt
     1681 ggagtttatt caccgggcac tctccgggat gcaagaggaa ctgacagcac tcggcagcac
     1741 cctcgatgtc aggtatggaa aacccttgca ggtgtaccag gaattgttca gggagtataa
     1801 tgtgacatcc gtttatacca atcacgatta tgaaccctat gcccttgaaa gggatgcggc
     1861 tatcgggcaa tggctgaaag caaagggttg ttcatttcag acgttcaagg accaggtgat
     1921 actggagaaa aatgaagtac tgaaggatga cgggaaaccc tatacagtat tcactcctta
     1981 ttcaaggaag tggaaggccg tgttgggggc gcaaacaatg cccgtattca acacaaaaaa
     2041 atatttcaaa cacttctttc agcaaccggc aaaagccatt ccttccctcg gttcaatggg
     2101 ttttaccgca acgggtcttc ctttcccggc caacagctgg aagaaagaag tcatcagccg
     2161 gtataaggaa caacgggata taccctccct gccgggaaca tcccgtttaa gtgtgcacct
     2221 gcgttttgga accatcagta tccgcgaact ggcccgtgaa gccggtgtgt tgaatgaaac
     2281 ctttttaaat gaattgatct ggcgggattt ttatcatatg attctctggc attttccagg
     2341 ggtcgtggga cattccttta aaccggagta tgacaagatc cggtggagaa ataatgaaca
     2401 cgaatttgac gcctggtgta aagggcagac cggttatccc attgtggatg cgggtatgcg
     2461 ggaactcaat gccaccggct ttatgcataa ccgggtccgg atgatcgtgg cctcctttct
     2521 gaccaaacat ctcctgatcg actggcgatg gggcgaaggt tattttgcgc ggaagctact
     2581 ggattttgac ctggcggcca ataatggtgg ctggcaatgg gcggcaggca gcggctgcga
     2641 tgcggctcct tatttcaggg tatttaatcc ctacctgcaa acacagaaat ttgatccgga
     2701 gctgaaatac atccggcaat gggtgcctga gtttgaagaa ctcacctatg cccggcctat
     2761 agtggtacat gaagtggcca ggaaaagatg cctggaaacc tatgcggcgg cgttaaagaa
     2821 ataaatccgg tgttattatc ttctcgttgc cctgcatgga atggtataca ttgaataatt
     2881 ccaaaattgc tttctttccg tcatcaccca gctgcaggga ataattattt acatagagat
     2941 cgatatgctg acgcatgacc tgttcactca tcgtttgcga gtgatctttt acatagccgg
     3001 tcacttccgg atagccggta aatgcatagg ccaggctttc cttaatcagg gtactgacct
     3061 gttttgccaa tggcgttccc aatgttcttt tcgcagcgat cccgcccagg ggaatcggca
     3121 ctttcatttt attttcccag tattctccca ggtccattac tttttttaat cctttatcct
     3181 ggtaagtaaa ccggttttcg tgaatgatca gtcccaggtc tgtttcaccg ctgaccaccg
     3241 cctcttcaat agcggaaaaa atgaaagctg tcttattcct tgcttccgga taagcataac
     3301 tgagcaagag gttggccgtg gtattgattc ccgggatggc aatccggctc ttgctgacat
     3361 ccggatttgt attttccgta gtgctgacca gtaatgggcc tacaccttta cccagggcac
     3421 tacctgaatc gagcagtaca taatgaggag ttgatttgaa aaaagcgggg aagctgagtt
     3481 tggtgatatc cagtttgcct tgcagggccc actggttcag cgtttccaca tcttccagta
     3541 tcacatcaaa ggaaagtccg ccggtgtcaa ttttctggtt gaccagtgcg tcaaatataa
     3601 aagtatcgtt ggggcagggg gagaagccca gggtcagttt cataatttgt ccagtaaacg
     3661 gatcaattcc gtgttcaggt taataatgga ggttttcata ttccagttct tcttattccg
     3721 ctccgcaata taatttgata cggcccttaa ttgaataaag ggcagttttt ccatcaggca
     3781 gacataatgc agggcggctc cttccatgga ttcaaccacg ggagcgaatt gttgttcata
     3841 aaatttcacc tttttccgat cagtggtgat ttcattgaca gaaatgccat tcactttttt
     3901 cagtttcact ttcttcagca cttcattttt attcaccagc catccttttg aaaaaggata
     3961 ctggttggca ggtacgagct tcaggtcaaa taatgttctc agtgtattca gctcgatcac
     4021 gctctgatcg gcaatcgctt cctgtttgat cgccaagacc gtgccggtag gaattcgccg
     4081 gtcaaagcaa ccgccaacac cggcctgtat cactagatct ggttttttaa tcaggatttg
     4141 ccgggtcagc gaccaggtgg tggccgtcag tccgatgcct gtgatcagta catccggctg
     4201 ccgggaaatg aggccgtttc gcagctgctc caggaatgga tttatttcaa tgggcgtggc
     4261 ggcgatgacc aggatattca tttctgctgt ttctgcaaag gtgcttaatt gttccctatc
     4321 cgggtcataa aaggggaagt ttctcgtacc tttgtgcccc gcagataaaa aaacggcggt
     4381 acctctccgc ccgaatgccg gaggggaaac tcaaaaagta aagcatggtt tacttaaccc
     4441 ggatcgaaca ttttaatgcg gctcacaagc tgtacaatcc ccgctggacc aaggaaaaga
     4501 acgaggctgt attcgggcgc tgtgccaatg aaaactggca tggacataat ttcgaattat
     4561 acgtaaccat taagggctat cctgatccgg acaccggatt tatcttcgat gccaaacaac
     4621 tgggtaaact ggtgcaggaa cggatcatcg agcaactgga tcaccggaac ctgaacgagg
     4681 atgtcgggtt tatgaaaggg aagatgtgtt ctattgaaaa cctcgtgatc ggtatctgga
     4741 atgagttgca gcctttatta ccggctgatg cacccttgca ttgcctgaaa ctggtggaaa
     4801 ccgataaaat ttacgtggaa tattatgggg agcaggaagc tgtgaacaaa tagttccgtt
     4861 ccctgttata acctgcgggt tagccgttca tatttcttaa attgtagcat gagtaacaag
     4921 gtggccgttt tccggaaaga gagttttaat gcagcgcacc ggttgtttaa tcccggctgg
     4981 gatcaggcga ccaatgaccg tgtatttggt aaatgtgctt tccccaatta tcatggacat
     5041 aattacgatc tggtagtgaa agtggtgggc gtaccggatc cggcaacggg gtatgtaatg
     5101 gacatgaaaa tactgagtga cctggtgcaa cgggaaatcg tggaacgctt tgaccaccgg
     5161 aacctgaacc ttgatacggt ggaattcagg aatctgaacc cgaccacgga aaatatcgcg
     5221 gtggttattt ataatttatt gcgcccgcat atcggaccag aactggaatt gcaggtgcgt
     5281 ttatatgaaa caccccggaa ctttgtggag tacccggttt aaatcaggaa attatggcgt
     5341 atagtaaaat tgaacagtat gatgaatcca ccaccagcgg acttatcaaa tcatataagg
     5401 atgtattggg gctgatcggt gaagatgcgg aaagagaagg gttacaaaaa accccggaac
     5461 gggtggccaa agccatgcag tatctcacac aaggatacca gctggatgca catgcgattc
     5521 tgaattccgc aaaatttcat gaagaagtga gtgaaatgat cgtggtgaaa gatattgaac
     5581 tcttcagtat gtgtgagcac catatgctgc cgtttttcgg gaaagcccat gtggcctata
     5641 tcccgaatgg ctggatcacc ggtctgagta aagtggcccg ggtggtggat gtgtattccc
     5701 gccggctgca ggtgcaggaa agactgacca tccagatcaa ggatgccatc aaggaaacac
     5761 ttaatccgct gggagtggcc gttgtaatag aagccaaaca tctttgcatg atgatgcggg
     5821 gggtgcagaa acaaaactca gtaaccacca catctgcttt tgacggggaa ttccataaaa
     5881 actccacccg gagtgaattt atgaaactga tcggctcccg attaagctga gaatgaataa
     5941 tatttgcgga aatgccataa agatttttct aattaaaacc tttatggcat ttttgtttct
     6001 ataactacca gctaccatga aacacgcgtt tatttttccc ggtcaaggat ctcagtttcc
     6061 gggaatggga aaggatcatt atgagaacag tgcctttgcg aaaaaattat ttgaacaggc
     6121 gaatgagatt ttagggttca gaatttcgga tacgatgttt ggcggtactg aagaagacct
     6181 caaacaaacg aatgtgacac aacctgccat attcctgcac tcggtaattg catttaagag
     6241 tattgaaagt gcccggccgg atatggttgc tggtcattca ttgggtgagt ttaccgcgct
     6301 ggtggccaac ggaaccctga gttttgaaga tgcattgcaa ttggttgccg tacgtgccac
     6361 cgccatgcaa aaagcctgcg aagtgaatcc ctctaccatg gccgccgtac tcaacctggc
     6421 ggatgagaaa gtagaaggga tctgtgcgga agttcaacag gaaaccgggg agattgtcgt
     6481 tccggctaat tataattgtc cgggtcagct cgtgatcagt ggttcaagaa aaggcattga
     6541 tattgcttgt gaaaggatga aagccgctgg agctaaaaga gctcttgtac tgcccgttgg
     6601 tggtgctttt cattccccgc tgatgttgcc ggccaaagaa gaattgcagg caaaaatttt
     6661 atctaccaaa ttccattcgc ccacctgtgc catttaccag aatgtagcag caaaagccgt
     6721 gatggataat gaagagatca aacaaaacct ggtagatcag ttaacgggtg cggtgaagtg
     6781 gacccagagt gtgcaggcga tgattgccga cggagccacc cgttttaccg aggcaggacc
     6841 aggtaaagta ctgcagggac tggtgctcaa aatcaataaa gaaatgcagg tggaaggtgt
     6901 gaactgatca atggataaaa aagaactatt caaaaacacc cggaactttc cgggtgtttt
     6961 ttgcttatac agctgactgt ttaataacaa ccatgtttgc ggctctctgc actgaagtag
     7021 cgtttgatca ggttgaaaag ggccgggtca tagctctgca actcagtcct ggtgtttatt
     7081 tcgttatgta ctccattggc tgacgggaca tccacattac agttgaagta actctgcacg
     7141 ccttcggccc agtat
//
LOCUS       DAOPMW010000145         7111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_11973_length_7111_cov_8.411423, whole genome shotgun sequence.
ACCESSION   DAOPMW010000145 DAOPMW010000000
VERSION     DAOPMW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11973_length_7111_cov_8.411423"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLU11_08205"
     CDS             complement(<1..1171)
                     /locus_tag="PLU11_08205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPN59140.1"
                     /translation="METYGKILLIAMPAFLVLVLFEKWWGWRKGFDTVRNMDMISSLS
                     SGITNVTKSVLEITIAIGIYPFMLQYLALTQIENTVLVYILTFIIIDFNGYWVHRWSH
                     EVNLFWNLHVIHHSSEEYNLACALRQSISAFFNMFTVLLLPAALLGIPEQVIATVAPL
                     QLFAQFWYHTRHINKMGFLEKIIVTPSHHRVHHAVNPEYMDKNLSQIFIIWDKLFGTF
                     QEELKEVPPVYGISRPAKTWNPIRINFQHLWLMIKDAWRTNNWKDKFTLWFRRTGYRP
                     ADVAAKYPVYKIEDVYHFEKYDTRTSPAFTAWAWVQLTLLLLSISYLFGNIAYINSID
                     SSYIYWYGAFVFLTVYALTELMDRNSNAMIWEVLRCGLGCWFLYQQGDWFGAGFWL"
     gene            1447..2457
                     /locus_tag="PLU11_08210"
     CDS             1447..2457
                     /locus_tag="PLU11_08210"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HPN59141.1"
                     /translation="MAKYIFDCVDAHTCGNPVRLVKKGGPELLGKSMSEKRQHFLREF
                     DWIRTGLMFEPRGHDMMSGSILYAPHDPANDVAVLFIETSGCLPMCGHGTIGTITIAI
                     EEGLIKPKVPGIVRMEAPAGLVMISYKQDAASGKVESVKLTNVPAFLHSAELTAECPE
                     LGELVVDVSYGGNFYAIVDVQKHFKGLEHYAADKLIAWARELRKNINAKYSFVHPDDP
                     TINGCSHILWAGAVLDQNSTARNAVFYGDKAIDRSPCGTGTSARMAQWYAKGKLKKGD
                     EFIHESIIGSKFTGRIEEELTVHGKPAIRPSVEGWAKVYGYNTIWIDKEDDPYAYGFQ
                     VI"
     gene            2571..3905
                     /locus_tag="PLU11_08215"
     CDS             2571..3905
                     /locus_tag="PLU11_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59142.1"
                     /translation="MSSRKRFILQAGALTAGTMLANSVGNNVFAIFKNRIAPSDQINV
                     GVIGVNGMGWSDLKAMLKVPGVNLVALCDVDKNVLDKRMGELKAMNVDASKVRTYADH
                     RALLDQKDIDAVIIGSPDHWHCLQMVDACQAGKEVYIEKPVGNSIEECRIMIAAQKKY
                     NRVVQAGQWQRSQQHFQEAVNFVHSGQLGNIRAVKVWCFVGWKKPIPFIADSPVPAGV
                     DYNRWLGPAEIRPFNANRFHFTFRWFWDYAGGLMTDWGVHLLDYALIGMKATVPKSIS
                     AMGGNFGDPASPQETPDTLTALYQFDKFSLSWDHAIGIENGLYKQNHGVSFIGDNGTL
                     ELDRSGWEVMEEPRSPKKVLVERKKSMDNGLDKHMENFVSVIRSRKMEDLKCSIQEAS
                     HIAMLSQMGNIAFRSGKKLDWDQQRGRFTNEKINRKYLAAPYHNGYKLPKIG"
     gene            3938..4885
                     /locus_tag="PLU11_08220"
     CDS             3938..4885
                     /locus_tag="PLU11_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN59143.1"
                     /translation="MLVVLACAALLKFRSGNQKPFAPADALPDEFIVVLGVAQDAGYP
                     QIGCEKECCEAYWKGKEEKKLVTCLALVDRPSNKYWLIEATPDITVQLKNLQPFLPAK
                     PDYSPDGIFLSHAHTGHYTGLLQLGREAMGANEIPVYVMPRMDSFLRNNGPWSQLVQI
                     KNIALTGVKEDSLVKLSGQLSITPWKVPHRDEYSETIGFVISNGKKKILFIPDIDKWE
                     HWDRDIKDLVMDFSVVLIDGTFYKDGELPGRNMKEVPHPFVEETARLMSGLPDTVKTK
                     IHFIHFNHTNPLLKKASKEKDQVRAAGFNVAEEGMIFKL"
     gene            4896..6872
                     /locus_tag="PLU11_08225"
     CDS             4896..6872
                     /locus_tag="PLU11_08225"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPN59144.1"
                     /translation="MRKGILVPLLAIAVAAQAQTSLKQFEFVNFSQVNVTDQFWKPKI
                     DKVATKTLDACIYQTETATPRIRNFERVARAKGEEHEGIFYDDSDVFKALEAMAYSLK
                     THPSEAMEKKCDEWIEKIASAQQPDGYLNTWYTLKGLQDRYTDMSMHEDYNAGHMIEA
                     GVAYYNATGKRKLLDVCIKWADHFGAIFGPGKKHWVTGHQELELALVKLYKITNNIAY
                     LNLSDWLLSERGKKLAKGYTWTDWKDTAYAQDVLPVKEQKEITGHAVRAMYMYTGAAD
                     VAAQTGDTAYLRAMRTVWEDVVYRNMYITGGIGSAGSNEGFSVDYDLPNEQAYCETCA
                     SVGMVFWNQRMSALTGNAEYMDVLERSLYNGALDGLSLSGDRFFYGNPLASKGQHQRR
                     EWFGTACCPANIARLVASLGDYIYAYGDNAVYVNLFVGSNTSFNLKNGPVGLKMETNY
                     PWEGKVKISIDPVKKSNFSLHLRVPGWVQREVAPGGLYAMLPVSEGFESVSSTSIVKL
                     NGKEIKYKIENGYMVMAQDWRKGDQLEFEFPMEVKIIKARTEVKQDQGRIAIQRGPLV
                     YCVEGADNNGKAWNMIVPKNTKFESIDYKVLDEYVKAITAEVPVVTVGEDGQSLITEK
                     KKIIAIPYYAWANRGKNEMQLWLPTRINEVKVNY"
     gene            6972..>7111
                     /locus_tag="PLU11_08230"
     CDS             6972..>7111
                     /locus_tag="PLU11_08230"
                     /inference="COORDINATES: protein motif:HMM:NF027657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4964 domain-containing protein"
                     /protein_id="HPN59145.1"
                     /translation="MKYNLIILLLFGTITAYSQVNKAPAYPLITHDPYFSIWSTTDKL
                     NES"
BASE COUNT         2022 a         1396 c         1920 g         1773 t
ORIGIN      
        1 ccagccagaa ccccgcccca aaccaatctc cctgctggta aagaaaccag caccccagtc
       61 cgcaacgcag gacttcccag atcatggcat ttgaattccg gtccatcagt tcagtcagcg
      121 cgtagacagt caggaataca aaagcaccgt accaatagat atagctgctg tcaatcgaat
      181 tgatataggc aatattgcca aagaggtaac tgattgagag caagagcagg gtcagttgta
      241 cccaggccca ggcagtgaag gccggggagg tacgggtatc atatttttca aaatgataaa
      301 cgtcttcaat tttatatacc ggatacttag ctgctacgtc agcgggacgg taaccggtac
      361 gcctgaacca gagggtaaat ttgtccttcc agttattcgt ccgccaggca tctttgatca
      421 tcagccagag atgctgaaaa ttgattcgga tcgggttcca ggttttggcc ggccgggaaa
      481 tgccatacac gggcggcact tcttttaact cttcctggaa ggtgccgaat aatttatccc
      541 agataatgaa gatttgcgac aggttcttat ccatgtattc cgggtttacg gcatggtgta
      601 cgcggtggtg actgggggta acaatgattt tttccagaaa ccccattttg ttgatatgcc
      661 gggtgtggta ccagaactgg gcaaataatt gaagcggggc aaccgtagca atcacttgtt
      721 ctggaatacc cagcagggcg gcaggcagga gcaatacggt aaacatatta aaaaaggcag
      781 aaatgctttg ccgaagggca caggccaggt tgtattcttc actgctgtga tggatcacat
      841 gcaggttcca gaacagattg acctcatgac tccagcggtg cacccagtac ccattaaaat
      901 caataattat aaaagtcagg atgtagacca ggactgtatt ttcaatttga gtcagggcca
      961 gatattgtaa cataaaggga taaatcccga tggcaatggt gatctccaat acacttttgg
     1021 tgacattggt aatccctgaa ctcagggaag agatcatgtc catattccgg accgtatcaa
     1081 atccctttct ccagccccac catttttcaa aaagtaccag cacgaggaag gctggcatgg
     1141 caatgagtag tatctttccg taggtttcca tgaacaagca attggaggct aatttagaga
     1201 aagttaatag aaaatggaaa atagaaaata ggaggtgaaa gtggtgagtg gtgagtggtg
     1261 agtagggagt gggatgtact aagtcgttct gtcatcccga aacgaagtga gggatggaat
     1321 ggccctgcag gggcctgata attaatagcc ccgggtgaaa cccggggtaa acggcagcca
     1381 ggtcccctga accctgaagg ggttcaacta aactaagaag agtagaaaat tcatattgag
     1441 atatttatgg ctaagtacat atttgactgt gtggatgcac acacttgtgg taatccggta
     1501 cgattggtaa aaaagggagg acctgaactg ttgggtaaaa gcatgagcga aaagcgccag
     1561 cattttttgc gggagtttga ctggatccgg accggactga tgtttgaacc ccgcggacat
     1621 gatatgatga gtggcagtat tctctatgct ccgcatgatc ctgccaacga tgtagccgtt
     1681 ttgtttattg aaaccagtgg ttgtctgccc atgtgcggtc acggtacgat cggaacgatt
     1741 acgatcgcta tagaagaagg attgataaaa cccaaagtgc ctggtattgt ccggatggaa
     1801 gctcctgccg gtcttgtaat gatttcttat aaacaggatg ctgcaagcgg aaaagtggag
     1861 agtgtgaagc tgacgaatgt gccggcattt ttacattcgg ctgagttgac ggctgaatgt
     1921 ccggaattgg gcgagctggt ggtggatgtt tcgtatggcg gtaattttta tgcgattgtg
     1981 gatgtgcaga agcactttaa aggactcgaa cattatgccg cggataaact gattgcctgg
     2041 gcccgggaat tgcggaagaa tattaatgcg aagtacagtt ttgtgcatcc ggatgatccg
     2101 acgatcaatg gatgctcgca tatcctatgg gcaggggcgg tactggatca gaactcgact
     2161 gcccggaatg cggtgtttta tggagataaa gccattgacc gttccccctg tggtaccggc
     2221 accagtgcca gaatggccca atggtatgcc aagggtaaat tgaagaaagg ggatgaattt
     2281 atccatgaga gtattatcgg gagtaaattt accggacgta ttgaagagga actgactgta
     2341 catggcaaac cggcgatccg tccctccgta gaaggttggg caaaggttta tggctacaat
     2401 acgatctgga tcgataagga ggatgatccg tatgcgtatg gattccaggt aatatgatcg
     2461 ggtgtcaggt gtcaggaata acccgtacgt aacattaaac ctcttgaacc ttttaaacct
     2521 tttaaacctc ttaaacctct taaacttatt caacctttca actactacct atgtcatcca
     2581 gaaaaagatt catactccag gccggtgcct taaccgcagg tacgatgttg gcgaattcag
     2641 tagggaacaa tgtgtttgcc atatttaaaa acaggatcgc tccttctgat cagatcaatg
     2701 tgggtgtgat tggtgtgaat gggatgggat ggtctgatct gaaagcgatg ctgaaagtac
     2761 ccggggtaaa cctggtggcg ctttgtgatg tggataaaaa tgtactggac aaacgcatgg
     2821 gcgagctgaa ggcgatgaat gtggatgcat cgaaagtcag gacctatgcc gatcaccggg
     2881 ccctgctcga tcagaaggat attgacgcgg tgattatcgg ttcaccggat cactggcatt
     2941 gtctgcagat ggtggatgcc tgccaggcag gtaaggaagt gtatatagaa aaacccgttg
     3001 gtaactccat cgaggaatgc cggatcatga ttgcggcaca gaaaaaatac aaccgggtgg
     3061 tacaggccgg tcagtggcaa cgcagtcagc aacattttca ggaagcggtg aattttgtgc
     3121 atagcggaca actgggcaat atcagggccg tcaaagtctg gtgctttgtt ggttggaaaa
     3181 aaccaattcc ctttatagcg gatagtccgg tacctgcggg tgtggattat aaccgctggc
     3241 tgggtccggc agaaatccgt ccattcaatg ccaaccgttt tcattttacg ttccgctggt
     3301 tctgggatta tgccggcgga ctcatgaccg actggggggt acacctgctg gattatgcat
     3361 tgattggaat gaaggccacc gtaccgaaat ccatcagtgc catgggcggt aatttcggag
     3421 atccggcttc gccacaggaa acaccggata cactgactgc tctttaccag tttgataaat
     3481 tcagtttatc ctgggatcat gcgatcggta ttgaaaacgg tttgtacaaa caaaatcacg
     3541 gtgtttcatt tataggcgat aatggcaccc tggagctgga ccgcagtggc tgggaagtga
     3601 tggaagaacc ccggagtccg aagaaggttt tagtagaaag gaaaaaatcg atggataacg
     3661 gattggataa acacatggaa aactttgtca gtgtgatccg ttcccggaaa atggaagacc
     3721 tcaaatgttc gatccaggaa gcatcgcata ttgccatgct gagtcagatg ggaaatatcg
     3781 cattccgtag tggtaagaaa ctggattggg atcagcagcg tggtcgtttt accaatgaga
     3841 aaattaaccg taaatacctg gcggctcctt atcataatgg ctataagctc cctaaaatcg
     3901 gatagaaatg catacacggg gctttaaact ttttttagtg ttggtcgtac tggcctgtgc
     3961 ggctttatta aaattcagat ccggcaacca aaagcctttt gcacctgctg atgcacttcc
     4021 ggatgaattt attgtggtgc tgggggtggc acaggatgca ggatatccgc agattggttg
     4081 tgaaaaagaa tgctgcgagg cctattggaa aggaaaggaa gaaaaaaaac tggtaacctg
     4141 cttggccctg gtagaccggc catctaacaa atactggtta attgaagcga ctcccgatat
     4201 cactgttcag ttaaagaacc tgcagccatt tctacccgca aaaccggatt acagtcccga
     4261 tggtattttc ttatcgcacg cccataccgg acattatacc ggattgctgc agcttgggcg
     4321 ggaagccatg ggggcgaatg aaattccggt gtatgtaatg ccccggatgg attccttcct
     4381 ccgtaacaat ggcccctgga gtcagctcgt tcaaataaag aatatcgcat taaccggggt
     4441 aaaagaagac agcctcgtta agctatccgg ccaattaagt attaccccct ggaaagttcc
     4501 gcaccgggat gagtacagcg agaccattgg ttttgttatc agcaacggga agaaaaaaat
     4561 actttttatc ccggacatag ataaatggga gcattgggac cgggatatca aagacctggt
     4621 gatggatttc agtgtggttt tgatcgatgg cactttttat aaggatgggg aattaccagg
     4681 tcgcaatatg aaggaagtgc cacacccgtt tgtggaggaa accgcccggt tgatgtccgg
     4741 gttaccggat acggttaaaa caaaaattca ttttatccat tttaatcata ccaaccccct
     4801 tttaaaaaaa gcatcgaagg agaaggatca ggtccgggct gccggtttta atgtggcgga
     4861 agaagggatg atttttaaat tataatttaa ccaacatgag aaaaggaata ttggtgccat
     4921 tgctggctat tgctgtggca gcgcaggcgc aaacttcact taaacaattt gagtttgtca
     4981 atttttccca ggtgaatgtg acagatcagt tctggaaacc caagatcgat aaagtggcca
     5041 ccaaaacact cgatgcctgt atttaccaga cagaaaccgc tacgccccgt atccggaatt
     5101 ttgaacgggt ggcccgcgct aaaggggagg aacatgaggg cattttttat gatgacagtg
     5161 atgtgttcaa agcgctggaa gcaatggctt attccttaaa gacacatccc agtgaggcta
     5221 tggaaaagaa atgcgatgaa tggatagaaa aaatcgcatc cgcccagcaa ccggacggct
     5281 acctgaatac atggtacacc ctgaagggat tgcaggaccg ctatactgat atgagcatgc
     5341 acgaggatta caatgccggt catatgattg aggccggtgt ggcgtattat aatgccaccg
     5401 gaaaacgtaa gctgcttgat gtctgtatca aatgggcgga tcatttcgga gccatttttg
     5461 gtccgggtaa aaagcattgg gtgaccgggc accaggaact ggaattggcc cttgtgaaat
     5521 tatataaaat caccaataat attgcttacc tgaatttgtc ggattggttg ttgagcgaaa
     5581 ggggaaaaaa actggcgaaa ggatatacct ggacggattg gaaggatacg gcttatgcgc
     5641 aggatgtatt accggtgaag gagcaaaagg aaattaccgg gcatgcggta agagctatgt
     5701 atatgtatac gggtgcggca gatgtagccg cacagacagg tgatactgcc tacctgagag
     5761 ccatgcggac cgtttgggaa gatgtagtgt accgcaatat gtatatcaca ggaggcattg
     5821 gttcggctgg tagtaatgaa ggcttcagtg tggattatga cctgcccaat gaacaggctt
     5881 attgtgaaac ctgtgcttcg gtggggatgg tgttctggaa ccagcggatg agtgcgctga
     5941 ccgggaatgc cgaatacatg gatgtgctgg agcgaagctt atataacgga gcattggatg
     6001 ggttaagcct gagtggtgat cgttttttct atggcaaccc gttggcgtca aaaggacaac
     6061 atcaacgcag ggaatggttc ggtacagctt gttgtccggc caatattgca cgactggtgg
     6121 cttcattggg tgattatatt tatgcgtatg gggacaatgc tgtttatgta aacctgtttg
     6181 tgggaagtaa tacttctttt aatctgaaaa acggaccggt tggattgaag atggaaacca
     6241 attatccgtg ggaagggaaa gtgaaaatca gtatagatcc ggttaaaaaa tcaaacttca
     6301 gtttgcacct ccgggtgcct ggttgggtgc aacgggaagt ggcaccgggt ggtttatatg
     6361 ctatgcttcc tgtgagtgaa gggtttgaaa gtgtaagcag tacttcaatc gttaaactga
     6421 atggtaagga aattaaatac aaaattgaga atgggtatat ggtcatggcc caggattggc
     6481 gaaaaggaga tcagctggaa tttgaatttc cgatggaggt taaaattatt aaagcccgta
     6541 cagaagtgaa gcaggatcag ggccgcatag caattcagcg gggcccgctc gtgtattgtg
     6601 tggaaggggc ggataacaat gggaaagcct ggaatatgat tgtacccaag aacacaaagt
     6661 ttgaatccat tgactataaa gtgttggatg agtatgtaaa agcaattacg gcggaagtgc
     6721 cggtagtgac cgtgggagaa gatggccagt ctttaataac agagaaaaag aaaattatcg
     6781 ccatcccgta ttatgcctgg gccaaccggg gaaaaaacga aatgcagctg tggttgccga
     6841 caaggattaa tgaggtgaag gtgaattatt agggtgagtg gtgagtggtg agtagtgagt
     6901 ggagaagcct gacttgccct ccgaagtgga acgtaggagg gagggctgat attattatat
     6961 aaaaaccggt tatgaaatac aacctcatta ttttattatt attcggaact ataacagcat
     7021 attcccaggt caataaagcg cccgcatatc cgctgattac ccatgatcct tatttcagta
     7081 tctggagcac aacagataaa ctcaatgaat c
//
LOCUS       DAOPMW010000146         7093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12020_length_7093_cov_7.497869, whole genome shotgun sequence.
ACCESSION   DAOPMW010000146 DAOPMW010000000
VERSION     DAOPMW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12020_length_7093_cov_7.497869"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLU11_08235"
     CDS             <1..452
                     /locus_tag="PLU11_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59146.1"
                     /translation="LRLAYHGLRAKDNSFNATMKTDFDPSIGNINIVPQDIGRVILNL
                     ISNAFYAVNERLRQAQPDNEYEPIVEVSTKKEGKNVFISVKDNGNGVHAKVLDKIFQP
                     FFTTKPTGQGTGLGLSLSYDIVKAHGGELKVDTEEGKGSEFIITLPV"
     gene            465..1766
                     /locus_tag="PLU11_08240"
     CDS             465..1766
                     /locus_tag="PLU11_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59147.1"
                     /translation="MMTEKERLLNETHQQFMETGLNSPEKLDIMDHLTSPDLMGFGTT
                     IDERIFSVADLKSLITRQREQSQGMEIVWTINPVLRKVTHGGNVAVYVDEITLELTVN
                     NEKIKIALRYSNVLEYRDDKWVVIHFHGSKPENVATEEDTWGIEEWKTKNAALEKLVK
                     ERTAELEKSLIELKATQAQLIHSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNSELI
                     SELVDEIDNGNTAEVKLIAADIKENSGKINHHGKRADAIVKGMLQHSNISSGKKEPTD
                     INALSDEYLRLSYHGLRAKDNSFNATMKTDFDPSIGNVNIVPQDIGRVILNLISNAFY
                     AVNEQSKKGIQGYLPTVEVGTKRTGNKIEVMVKDNGNGIPARVLDKIFQPFFTTKPTG
                     QGTGLGLSLSYDIVKAHGGELKVDTIEGECTIFTIELNVTG"
     gene            1768..3855
                     /locus_tag="PLU11_08245"
     CDS             1768..3855
                     /locus_tag="PLU11_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59148.1"
                     /translation="MKRILIFFILFIPVQCLLAQEQTYFVQWTNKGADSAKVLFRVSD
                     NDSVRMKLARSLGLYYQEKDRDTSLFYTLKQLQLAKKLKQRFWEADAYDASGWLLSQL
                     KNYPLSLTHLLNARKIAEDKSTEENIWNLSWFSPKGDARYARLTSLGFIHNDLSQLYN
                     QMGDRQRELAQLYEGLKIGGLINNSELLGLLRSNLSQIYVSMNKPDSAIYFGEQALVD
                     MRQSGYKTYEGHQLKLIGEAYVSKNDFARARKYLDQSILANEANHSIAKLPEAYFPYA
                     EICKLQNKPDSGIYYLKSALMAYHLLGEPEKRKRVYASLYDLYVITGNRDSSFHFLNL
                     YRLLSDSLNQDEKSKILAYQNVAFDEQIALQQQESLKIKKANTNRTYALIAGIMVLLS
                     IAFLLFRNNRIRQKANQLLTIQKKEIELQKIQVESTLLELKSTQSQLIQAEKMASLGE
                     LTAGIAHEIQNPLNFVNNFSEVNSELISELVDEVDKGNTAEVKLIAGDIKDNSEKIKH
                     HGKRADAIVKGMLQHSSSGSGKKEPTDINALADEYLRLAYHGLRAKDNSFNATMKTDF
                     DPSIGNINIVPQDIGRVILNLISNAFYAAPLPPKGGFSDPDYRHVPTVWVSTKREADK
                     VIISVRDNGPGIPAEIRDKIFQPFFTTKPTGQGTGLGLSLSYDIVKAHGGELKVETKD
                     GEGSIFIIQLPTV"
     gene            3865..>7093
                     /locus_tag="PLU11_08250"
     CDS             3865..>7093
                     /locus_tag="PLU11_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN59149.1"
                     /translation="MKKYSWLLLLAGMLLSSGLVFSQQLKFNQVLGNKTFELGYEQVI
                     TQDQKGFIWFTGVNKGLHRYDGKKTTLFSHNPDNTNSLASNLAIALAADSAGNIWTGT
                     IGYGVDRFNPETKKFTHFRHSPTDPKSLSNDTVTAILTDRSGNIWIGTTRAVDKFDPA
                     TGIFTHYIINELASYVSSADNVAGITTLFEDSKGHLWTGWTEFTGKIKKDGMGGLARL
                     DQATGKFIQYKHDPGNPNSLASNMVYAVYEDRKGNLWVGTSGNGLQILDRVSGKFSNY
                     QFDAANLDKPSSPPVSGGQNDFTSFITEDVKGRLWIGSMTSGINMYDPFLKRTVHFGR
                     VSNDITNRYAKDTLTGFTDSSALRAFTSKDGLLWIVSRSKGIYNIDFSSTTVPYTPLN
                     ASASSFYFEEEKNMLWINSDSGLIRKNVLTGEQKLIRQDPRNPTSFLSKEVMSMVGDG
                     EGNIWLANHFNGVDKFSIKTEQITHYKHDKNNPASLVHDTTHILFFDREHYLWIGTHK
                     GLSRMDPKTGVCTNYTHNPKDSFGISKSHINDIVQEKNGRIWLATDNEIFALDIKTEK
                     FQRYELSGGQIVNVCIDAAGIIWANCGGILFSFDQVKNKFKKFVTEIYPGGFDNVLGL
                     IEDNKGNLWVSTSRSIIKLNSKRDAATIFGTAQGVQSSTELWLRNFKAKDGRLYLGSN
                     KGYYSFKPDELKDERRPPILSFSSFKIGNQLVLAEDGTVLTEPIWKTNNIKLNYSQNT
                     ISFEFGAIDYESPGNITYLYKLENYDEEWRDLGTETQATFFNLPPGNYTLHVKAINSD
                     GAVVSKSIAIDITPPWWKTWWAYIFYGLLVILAGYLIYKYQKNYIVKRERERTQQKEL
                     AQAREIEKAYTQLKSTQAQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNTELI
                     SELMEEVDKGNTAEVKSIATDIRENSEKIVHHGKRADAIVKGMLQHSRSSSNQKEPTD
                     INALADEYLRLAYHGLRAKDKSFNATMHTDYDATIGSIQIVSQDIGRVVLNLITNAFY
                     VVNEMKTLRQAQGDNNYQPLVSVSTRKLDGKVEIKVSDNGNGIPQK"
BASE COUNT         2247 a         1367 c         1702 g         1777 t
ORIGIN      
        1 acctgcggct ggcctatcat ggactccggg ccaaggacaa ttcctttaat gccactatga
       61 aaacggattt tgacccgtca atcggcaata tcaacatcgt acctcaggat attggccggg
      121 tgatccttaa cctgatcagt aatgctttct atgcagttaa cgaacggctt cggcaagctc
      181 agcctgacaa cgagtatgaa cccattgttg aagtaagtac gaagaaggaa ggcaaaaacg
      241 tttttataag tgtaaaggac aatggcaatg gagttcatgc aaaagtactg gacaaaatat
      301 ttcagccttt tttcacgacc aagcctaccg gccagggaac gggtttgggg ttgagcctga
      361 gttatgatat tgtgaaagcg catggcgggg agctgaaggt ggacaccgag gaaggcaagg
      421 gaagtgaatt tattatcacc ctgcctgtat aaattatagt agttatgatg acagaaaagg
      481 aaaggttact caacgaaaca caccagcaat tcatggaaac cggacttaat agtccggaga
      541 aattggatat aatggatcat ttaacgtcac cggatcttat gggatttggt actacaattg
      601 acgaaagaat attttcggtg gctgacctga aaagtctcat tacccggcaa agagagcaaa
      661 gtcagggtat ggagatagtg tggacaataa atcctgtatt acgtaaagta actcatggcg
      721 gcaatgtggc cgtttatgtg gatgaaatta cgcttgagtt aacggttaat aatgaaaaaa
      781 taaaaatagc gttgcgctat agcaatgtgc tggaataccg ggatgataaa tgggtcgtga
      841 tccattttca tgggtctaaa ccggagaatg ttgccacgga agaagatacc tggggtattg
      901 aagagtggaa aaccaaaaac gcggcacttg aaaaactggt aaaagaacgt actgctgaat
      961 tggaaaaatc gctgattgag ctgaaagcca cgcaggccca gctgattcat tccgagaaga
     1021 tggcctctct cggagaactc accgccggca tagcccatga aatccagaat ccgttgaatt
     1081 tcgtcaataa tttttcggaa gttaacagtg aattgatcag tgaactggtt gatgagattg
     1141 acaacgggaa tacagcagaa gtaaagttaa ttgctgctga cattaaggaa aattccggaa
     1201 aaataaacca tcatggcaaa agagccgatg cgatcgtaaa agggatgctg cagcactcca
     1261 acatcagtag cggcaagaaa gaacccaccg atatcaatgc gctgtcagat gagtacctgc
     1321 ggctgtctta ccatgggctc cgggccaagg acaattcctt taatgcgacc atgaaaacgg
     1381 attttgaccc gtcaatcggg aatgtcaaca tcgtaccaca ggatatcggc cgggtgatcc
     1441 tcaacctgat cagtaatgct ttctatgcag taaatgaaca atcaaaaaag gggattcagg
     1501 ggtatttacc cacggttgaa gtaggtacaa aaaggacagg aaacaaaatt gaggtgatgg
     1561 taaaggataa tggcaatgga attcctgcaa gagtactgga caaaatattt cagccttttt
     1621 tcaccaccaa gcctaccggg cagggaacgg ggttggggct gagcctgagt tatgatattg
     1681 tgaaagcgca tggaggagaa ttgaaagtag atacgataga gggcgagtgt accatattta
     1741 cgattgagct aaatgtaacc ggataacatg aaaaggattt tgattttctt tattttattt
     1801 atccctgttc aatgtctgtt ggcgcaggaa cagacctatt ttgtgcagtg gaccaataaa
     1861 ggggcagata gtgctaaagt tttattccgg gtctcagata atgattcagt gcgaatgaaa
     1921 ctagcccggt cattaggatt atattaccag gaaaaagaca gggataccag tttgttttat
     1981 acgttgaaac agcttcagtt ggctaaaaaa ctaaagcagc gattttggga agcagatgcc
     2041 tatgatgcat cgggatggct tttatcacag ttaaaaaatt atccgctatc cctcacccat
     2101 cttttaaatg caaggaagat agccgaagat aaaagtacgg aggaaaatat ctggaatctt
     2161 tcctggtttt cacctaaagg ggatgcaagg tatgcccggc tgacttcttt aggctttatt
     2221 cataacgatc tctcccagtt atacaatcag atgggggaca ggcaacggga acttgctcaa
     2281 ctctacgagg gtttgaaaat cggtggactt attaacaata gtgaattact gggattactc
     2341 cgatccaatc tctcccagat ctatgtcagc atgaataaac cggattcggc tatatatttt
     2401 ggagaacagg cgctggttga tatgcggcag tcagggtata aaacgtatga gggacaccag
     2461 ttaaaattga ttggcgaagc atatgtcagt aagaatgatt ttgccagggc caggaagtat
     2521 cttgatcagt ctatcctggc caatgaagcc aatcatagca ttgctaaatt gccggaagct
     2581 tattttccat acgccgaaat atgcaaactg cagaacaaac ccgactccgg catttattat
     2641 ttaaaaagtg cgttgatggc ctaccatttg ctcggggagc ccgaaaaaag gaaaagggtt
     2701 tatgcctctt tatatgacct gtatgtcata actggaaacc gcgacagttc tttccatttt
     2761 ttgaatctat accggctgtt gagtgacagt cttaaccagg atgaaaaaag caagatactg
     2821 gcctatcaga atgtggcatt tgacgaacaa attgctttac agcagcagga aagtctgaaa
     2881 ataaaaaagg caaatacaaa ccggacatac gcattgatag ctggtatcat ggtgttgttg
     2941 tccattgcgt tccttttatt ccgcaataac cggatccgcc aaaaagcgaa tcaactgctg
     3001 accattcaaa aaaaggaaat tgaattacag aaaattcagg tggaaagtac attgctggaa
     3061 ttgaaatcca cgcaatccca acttatccaa gcagaaaaaa tggccagcct gggtgagttg
     3121 accgccggta ttgcccatga gatccagaat ccgttgaact tcgtcaataa tttttcggaa
     3181 gtaaatagtg agttgataag tgaattggtt gacgaggttg acaaggggaa tacagctgaa
     3241 gtgaaattga ttgcagggga cataaaagac aactctgaaa aaataaaaca ccacggcaaa
     3301 cgagccgatg ccatcgtaaa aggcatgttg caacattcca gcagtggcag cgggaaaaag
     3361 gagcctacgg atatcaatgc tttggccgat gagtacctgc ggctggccta tcatggactc
     3421 cgggccaagg acaattcctt taatgccact atgaaaacgg attttgaccc gtcaatcggc
     3481 aatatcaaca tcgtacctca ggatattggc cgggtgatcc ttaacctgat cagtaatgct
     3541 ttctacgcgg cccccctgcc ccccaaaggg gggttctcag accccgatta cagacatgtt
     3601 cccacagtct gggtaagtac aaaaagggaa gctgataaag taattatttc ggtcagggat
     3661 aatggacctg gtattcctgc cgaaatcagg gacaaaatat tccagccttt cttcaccacc
     3721 aagccaaccg ggcagggaac aggattgggc ttgagtctga gttatgatat tgtgaaagcg
     3781 catggcgggg agctaaaagt ggagacaaag gacggggaag gttcaatatt tattattcaa
     3841 ttaccaacag tctaaatcat tccaatgaaa aaatattcat ggttattgct tcttgccggc
     3901 atgctccttt catcaggact ggttttttcg cagcaattaa agtttaatca ggtgctgggt
     3961 aataaaacct ttgaactggg ttatgaacag gtaataactc aagaccagaa agggtttatc
     4021 tggtttacgg gagtaaataa gggattgcat cgttatgacg ggaaaaaaac cacccttttc
     4081 agccacaatc cggataatac caactcactg gcaagtaacc tcgcaatagc gctagctgct
     4141 gattctgctg gtaatatatg gaccggcacc attggttacg gggttgaccg cttcaatcct
     4201 gaaacaaaaa aattcaccca tttccggcat agtccaactg atccgaaaag cctgagcaat
     4261 gatacggtaa ctgccattct aaccgatcgc tctggaaata tctggatcgg tacaacccgg
     4321 gccgttgaca agtttgatcc tgcaaccgga atctttacgc attatatcat aaatgaattg
     4381 gccagctatg tgagcagtgc tgataatgtt gccggtatta ctacgctctt cgaagacagc
     4441 aaaggccatt tatggacagg ctggactgaa tttaccggaa agattaaaaa agacggtatg
     4501 ggcggattgg cccggctcga tcaggcaact ggaaaattta ttcaatacaa acacgatccc
     4561 ggaaatccca atagtcttgc cagcaacatg gtgtatgctg tttatgaaga tagaaaagga
     4621 aatttatggg taggtacgag tggaaatgga ttacaaatat tggacagggt gagcggcaaa
     4681 ttcagcaatt atcaattcga tgccgcaaac ctggataaac caagcagtcc gcctgtttcc
     4741 ggaggtcaaa atgattttac cagttttatt acagaggatg taaaaggcag gctctggatt
     4801 ggttcaatga ccagcgggat aaatatgtat gacccttttt tgaaaagaac tgttcacttt
     4861 ggtcgggtga gtaatgatat aacaaaccgc tatgccaaag ataccttaac tggttttacc
     4921 gatagttctg ccctgcgggc ttttacatcc aaagacggct tgctctggat tgtaagccgc
     4981 agcaagggta tttataacat tgatttttcc agtaccacgg ttccttatac gccgctcaat
     5041 gcatctgcga gttcatttta tttcgaagaa gaaaaaaata tgctttggat taattcagac
     5101 agtggactca tccggaaaaa tgtattgact ggtgagcaaa aattaatccg gcaagacccg
     5161 cgaaatccaa ccagtttctt gagcaaagag gtaatgagta tggtgggtga tggcgaaggt
     5221 aatatctggt tggctaatca ctttaacggg gttgataaat tcagtataaa aactgaacaa
     5281 ataacgcatt acaaacacga taaaaataac ccggccagtc tggtacatga cacaacacat
     5341 attttatttt ttgaccgcga gcattattta tggatcggta cacacaaagg gttgtcacgg
     5401 atggatccta agacgggagt atgtaccaat tacacgcaca accccaagga tagttttggt
     5461 atcagtaagt cgcatataaa cgatattgta caggagaaga acggcaggat ttggttggcg
     5521 actgacaatg aaatttttgc actggatata aaaacggaaa agtttcaacg gtatgagtta
     5581 agtggcggtc agatagtgaa tgtttgtatt gatgcggccg gtatcatttg ggctaactgt
     5641 gggggcattc ttttctcctt tgatcaggta aaaaataagt ttaaaaaatt tgttacagag
     5701 atatatcccg gcggatttga taatgttttg ggtttgatag aggataacaa ggggaattta
     5761 tgggtaagca caagcagaag tatcatcaaa ttaaacagca aacgcgatgc cgcaaccatt
     5821 tttggtactg ctcaaggcgt tcaaagttca actgaattat ggctgcgcaa tttcaaagca
     5881 aaagacggcc gcttatacct gggtagtaat aaggggtatt attcatttaa accggatgag
     5941 ctgaaagatg agcggagacc acccattttg agcttttcca gttttaaaat tgggaatcag
     6001 ttggtattgg cagaggatgg cactgtattg acggagccca tatggaaaac caataacatt
     6061 aaactgaatt acagtcaaaa caccatttca tttgagtttg gagccatcga ttatgaaagt
     6121 ccgggcaata taacctacct gtataaactg gaaaattatg acgaggagtg gagggatttg
     6181 gggaccgaga cccaagccac gttttttaac ttgccgccgg gcaactatac cctgcacgta
     6241 aaagccatca atagtgatgg tgctgttgtg tcgaaaagta tcgctattga tattaccccg
     6301 ccctggtgga aaacatggtg ggcctatatt ttctacggat tgttagtgat tctggccggg
     6361 tacctgatct ataaatacca gaaaaactat attgtcaagc gggaaaggga aagaacccaa
     6421 caaaaagaac tggcacaggc cagggaaatt gaaaaagcct acacacaatt gaagtccaca
     6481 caggcgcaac tgattcagtc ggagaaaatg gccagtcttg gggaactcac cgctggtatc
     6541 gcccatgaaa ttcagaaccc gctgaacttc gtgaataatt tttcagaagt aaacacggag
     6601 ctgatcagtg aattaatgga agaagtggac aaggggaata ccgccgaagt aaagtcgatt
     6661 gcgacagata tcagggaaaa ttcagaaaag atcgtgcacc atggtaaaag agccgatgcg
     6721 attgtaaaag gtatgctgca acatagccgc agcagcagta accagaaaga gcccacggat
     6781 atcaatgcac tggccgatga gtatttgcgg ctggcttacc atggattgag ggcgaaggat
     6841 aaatccttta acgccacgat gcatacggac tatgatgcaa ccattggtag tattcagatt
     6901 gtgtcgcagg atattggaag ggtggtacta aaccttatta ccaatgcttt ttatgtagtg
     6961 aatgagatga aaacccttcg gcaagctcag ggtgacaaca actaccaacc cctggtttcg
     7021 gtaagtaccc ggaaattgga tggtaaagtg gaaattaaag tgtccgataa tgggaatggc
     7081 attccacaga aag
//
LOCUS       DAOPMW010000147         7087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12041_length_7087_cov_11.655006, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000147 DAOPMW010000000
VERSION     DAOPMW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12041_length_7087_cov_11.655006"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLU11_08255"
     CDS             complement(<1..398)
                     /locus_tag="PLU11_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN59150.1"
                     /translation="MKKYHFILLLFIISFFSIMTNAQDTINYRFSFNHLALSVKDLQR
                     TVDFYKNVLHLKEIENRTKNEGIRWLSLGENKELHLISTTPGEVKITKAVHLALTSPR
                     FHDFVKELEAKKIPFTDWPGTPGKVHVRPDG"
     gene            complement(400..1818)
                     /locus_tag="PLU11_08260"
     CDS             complement(400..1818)
                     /locus_tag="PLU11_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPN59151.1"
                     /translation="MKQIFLLLCLFLQFTGYNQTCDCSAAIKTVQVNVESNLASYQHQ
                     VVEFKRENAYARHKLLINQEADSIRTLPECRNLLARYLSFIRDEHLFFRYTDDALPFS
                     SIADTAAVRQFYQDEPVYHFTKSNSPDTTLDGRWIFEDGSFSIQVFAHAAKRSYRIGV
                     MEKDDQLFWRKGQLKMELFKKGDGSLGCIYRRGTRLPTYYPVRIEKNVMYLGRELRFV
                     RYPEQVHAQSATETGLTFQPLSKQTAYLRIPSFDLSRQLAIDSIMDVNKDIIAGFNNL
                     IIDIRNNGGGGFEAFNALLPFVADSINIPQPYNASVWLSPENFSYYNESKYEYAETRE
                     DSLNEAAYIRFLEKYKGRFSPPDTGLQQLPFILHQPKKIALLYNRRTASTAEGFILLS
                     KESKRVKSYGENSAGAITYGDWRPVEIPALKVWIAMTTKKMVFLRPADLESRGIQPDM
                     PLDPNNETGWIRLVQADLENVK"
     gene            complement(2001..2213)
                     /locus_tag="PLU11_08265"
     CDS             complement(2001..2213)
                     /locus_tag="PLU11_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59152.1"
                     /translation="MKKQLRKWGLRLTATALLIAGLLLMILLNPILSYGNKTTYQQYS
                     IYHNKPIGDQFRPMLDEVTHLVKKSE"
     gene            complement(3198..6635)
                     /locus_tag="PLU11_08270"
     CDS             complement(3198..6635)
                     /locus_tag="PLU11_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPN59153.1"
                     /translation="MQLSVFSTDNQKSGFRLQYMEIFNWGTFDDTIHAIHPGGETSLL
                     TGANGSGKTTFVDALLTLIVPEKRYRFYNQSSGSEKKGDRTEDSYVLGGYGTISSEST
                     GSTKTLYLRENKDEAYSIILVNFANEAEQVVTLFQVRYFVNGEMKKHFGVAHKALKIE
                     EDFKPFDLGGAWKKTIDQRYNKGSRKFVEWFDAASRYAQRIVEVLGMQSIQALHLFNQ
                     TVGIKVLGNLDEFIRTHMLEPRNIEDQFQELKKHLSTLLDARRNIEKAEEQLKMLQPV
                     KDQYEQFQQLKQQKEELETLLNTAGIWKKYTQHELLRVASEQIRQQEKELVQKIAQHR
                     KELATLAEEERTILNQIDQNQAGQRLQQLEKSKQELEEKKNTASIRLEEFTKWCGTLH
                     LPETTAPDESAYLRIKKENQRKLLALNTEQRLNAEDEYTAKRKKDIATAEKEIVEKEL
                     NNLLVSKNNIPSYLIQIRNQLSEALDIVPHELPFAGELMQVRTEELDWQPAVEKLLRS
                     FSTRLLVPDKYYKKVNKYVNRENLRARFVYEHVTDNALVQHPEAGTVHEKLEFHPDHK
                     LGNWVEQQVIRQFNYACIDDEKTLDRYTRALTLNGLIKNGQRHEKDDRPDRNDPSRYV
                     LGWNNEKKKQALFKKRDQLSDELQQSAEITERCTNKGKRLQQQFYANSRIQEHAGFEE
                     LDIAGLQRGIHKVLEQINSLTKENKALTQLKKQLDDTRERKETAQLSQDQLVREEGVI
                     NQQLESLTQQLEGLKLIVDQITHEEKDLLLQFQQQQSEFMSLISLENLDTQFIAFRDA
                     RQSGLEKLKGSLQKTETGLSRAIYLIKNPSGALTQKYPDWSGDVVGFSEDAQYANEYI
                     EWMDKLSSENLPKYKRDFENYISDTITYKIGGLNEELDKWEREISNSIIRLNQSLSGI
                     NFNRMPDTYVQLRKQPVQAGSEIREFKMQLLDALPQAANWQQSSFEEKALHFTQKIQP
                     LIAELDASDSYRNKVMDVRNWFEFWADEKYRSNNETKKTYRQMGQLSGGEKAQLTYTI
                     LCSAIAYQFGITREGRASKSLRFIAVDESFSNQDEEKATYLMELCKQLHLQLLVVTPS
                     DKIQIVQDFIAHVHLVQRVQNRHSVIYNMTIKELKDQMEGGEPVILKEKKRSKKEVV"
     gene            complement(6647..>7087)
                     /locus_tag="PLU11_08275"
     CDS             complement(6647..>7087)
                     /locus_tag="PLU11_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPN59154.1"
                     /translation="EEDGYAYLLHVNPEEEDASVSWIIRRALTYEESIMLVLLREMMA
                     EFETGDSNNRELVKKRREIKEYAELFFKENASRVKFLKEIDRLIDKTEEYGFLAMTDD
                     HEIADEQRFRIKKIIKARVDAEELDSFYQQLKQHKEKLQKESNP"
BASE COUNT         1723 a         1705 c         1520 g         2139 t
ORIGIN      
        1 ccatcgggac gaacatgcac tttgccgggc gtgccgggcc agtcggtgaa cggaattttc
       61 tttgcttcca gttctttcac aaaatcatga aaccggggcg aggtcagggc caggtgcacg
      121 gctttcgtga tttttacttc cccgggtgtg gtggagatca ggtgcagttc tttgttttct
      181 cccagcgaaa gccagcggat gccttcgttt ttagtacggt tctcgatttc tttcaggtgc
      241 aagacgtttt tataaaaatc aacagtacgc tgaagatctt ttacagaaag ggcaaggtgg
      301 ttaaagctaa accggtagtt gatggtatcc tgggcgttgg tcattattga aaaaaaggaa
      361 atgatgaata aaagcagaat gaagtggtat tttttcatat tattttacat tttcaaggtc
      421 agcctgcacc agtcgtatcc agccggtttc attgttcgga tccaggggca tatcaggttg
      481 aatacctctg ctttccaaat cagctggtct taaaaatacc atttttttag tggtcatggc
      541 gatccagact ttcagtgccg gtatttccac cggacgccag tcaccatagg taatcgcgcc
      601 tgctgaattt tcgccatagc ttttaacccg cttgctttct ttactcaaca ggataaatcc
      661 ctctgcagta ctggctgttc tccggttata caacaaggca atcttttttg gttgatgcag
      721 gataaagggc aattgttgca ggccggtatc cggcggacta aaacggcctt tgtatttttc
      781 caggaagcgg atataagcag cttcattcag actgtcttcc cgtgtttccg catattcata
      841 tttggactca ttatagtaag aaaaattttc aggactcagc cataccgacg cattataagg
      901 ttggggaata ttaatactat ctgctacgaa aggaaggagt gcattaaatg cttcaaagcc
      961 acctccgcca ttgtttcgga tatcgatgat gaggttgtta aatccggcaa taatgtcttt
     1021 attgacatcc ataatagaat caatagccaa ttgtcgggaa agatcaaagg agggaatacg
     1081 gaggtaagcc gtctgcttac tcagtggctg gaaggtcaac ccggtctccg tggctgattg
     1141 tgcatgtacc tgttcaggat accggacaaa ccgaagttca cggcccagat acatcacatt
     1201 cttttcaatt ctgacaggat aataagtggg taatcgtgtg ccccgcctgt aaatgcagcc
     1261 caggctgcca tctccttttt tgaagagttc cattttcagt tgcccttttc gccagaacaa
     1321 ttgatcatct ttctccataa cgccaatccg gtaacttcgt ttagcagcat gggcgaatac
     1381 ctgaatactg aaactgccat cttcgaatat ccagcggccg tccagcgtag tatcagggct
     1441 atttgatttt gtgaaatgat agacaggctc atcctgatag aactgcctta ctgctgcagt
     1501 atctgcaatt gaactgaagg gcaaggcatc atcggtataa cggaagaaaa gatgttcatc
     1561 gcggataaag gaaaggtatc gggccagtag attgcggcat tccggcagcg tccggatact
     1621 atccgcttcc tggtttatca gcagtttatg acgggcatag gcattttccc ttttgaactc
     1681 aactacctgg tgttggtaag aagccaggtt gctttcgaca tttacctgta cagtctttat
     1741 tgctgcagaa cagtcacagg tttgattgta gccggtaaat tgcaaaaata agcagaggag
     1801 taaaaaaatc tgtttcataa aaaacggaat aaagccgtaa ggtaaatgat ttacagctat
     1861 ccggcatttc atatccgacc aattttaatc cggtattatc aacggagcgg tttaattctt
     1921 tttattttga agatgccatt ccatcatatc cagccgggta gcctcctcgc ttacagtatc
     1981 tttcaataat ccggaataac tcattcgctt ttcttaacca aatgggttac ttcatcaagc
     2041 atgggccgga actgatcccc gatgggctta ttatgataga tactgtattg ctggtatgtg
     2101 gttttgtttc cataactcag gatgggattt aacaggatca tcagtaaaag cccggctatc
     2161 agtaaggcgg tggccgtgag gcgaagtccc catttacgca attgtttctt cataattcct
     2221 gtttagatga agtgtactgg attcggtaag gtgcgaatga caattactta taggaataca
     2281 ataaatgtaa gtaaaattcc atggatttat gcccagaagc gacaaatgaa taattgtatg
     2341 ggcaggaatc accaaataaa ttataacaac actataaatt tagtcaagca ggcatttttt
     2401 acccaccggc tttaagtttt tgtgccaaag agtctccatg cttattctac tcgctttcct
     2461 gatcctttga ttaatagctg actttctgta cttgcattga agccctatat aaaaatacag
     2521 gaagacaacc gagatgaaat tgactggcca accagatttt taacacgcaa atagcctgac
     2581 tattgcctta actatggaga taaagcaaga attagcggta aaaaatttct gttcagatcc
     2641 ccatcaggaa tttgtgttcc tcttacctgg atttcttccg aagaggaaga gcagaagaac
     2701 accggcggca atctgaacga cgacgagtac attgcgtatc gtactatgtt cctgatcttt
     2761 tcccgcaata aagtaatgaa cagctggtgc aaaaatgcag ataatgacca gcaaaaggaa
     2821 aaaattccga tttttcatat ctgggcattt agggtgtttt tatgaaataa ttacagaaaa
     2881 tcagggtgta agcttattca aaagagtttc ttcattcctg ctgccagctc atatcctttt
     2941 gtacaacatt tgctgcagct aattccgggc agtagtttgg ctgactaaac aggtcatttt
     3001 tcgaaaccat ataaagttaa aacttatttt gaaaagcaac agtgcccgga gcaggatttt
     3061 caagaatccg ggaaaggaat aaactgtgtc ttttttaccc caaacagcaa aaaacacaat
     3121 tcagtgacca acagttataa gccggttaaa taccagcgga tgtgcttgag tctaatccgg
     3181 taaattcagt atttaagtta tactacttct ttctttgatc tttttttctc ttttaaaatg
     3241 accggctcac cgccctccat ctgatccttc agctccttga tggtcatatt gtagatcaca
     3301 ctgtgccggt tctgtacccg ctgcactaaa tgtacatggg caataaagtc ctgcacgatc
     3361 tggatcttat cactgggtgt tacaaccaac aactgcagat gcaactgctt acacagctcc
     3421 atcaggtagg tggctttttc ctcatcctgg ttgctgaaac tttcatccac cgcaataaag
     3481 cgcagactct tgctggccct tccctcgcgg gtaatgccaa actggtaggc aatagccgaa
     3541 cagagaatgg tataggtcag ctgcgccttt tcacctccgg acaattgtcc catctggcgg
     3601 taggtcttct tggtttcgtt attgctccgg tatttttcat cggcccagaa ctcaaaccag
     3661 ttccgtacat ccatcacttt attccggtag ctatcactgg catccagttc ggcaattaac
     3721 ggttgtatct tctgggtaaa atgcagggcc ttttcttcaa aactgctctg ctgccagttg
     3781 gccgcctggg gtaaggcatc gagcaattgc attttaaatt cacggatctc cgatcccgcc
     3841 tgtacgggtt gtttcctcaa ttgtacatag gtatccggca tccggttgaa attgatacca
     3901 ctcagcgatt gattcagcct gataatactg ttactgatct cccgctccca tttatccagc
     3961 tcctcattca gtccaccgat tttatacgtg atggtatcac tgatataatt ttcaaaatcg
     4021 cgtttatact tcggcaggtt ctccgaactc agcttatcca tccattcaat atactcattg
     4081 gcatactgcg cgtcttcgga gaagcccacc acatcgccac tccagtccgg atacttctgg
     4141 gtcaacgcac ccgaagggtt tttgatcaaa tagatcgccc ggctcagccc ggtctcggtt
     4201 ttctgcaaac tgccttttag tttttccaac ccggactgcc gtgcatcccg gaaagcaata
     4261 aactgcgtat ccaggttctc caggctgatc agactcataa actccgattg ctgctgctgg
     4321 aactgcaata acagatcctt ctcctcatgc gtgatctggt ccacaatcag tttcaatcct
     4381 tccagctgct gtgtaaggga ttcgagttgc tgattgatta ccccttcttc ccgtaccagc
     4441 tgatcctggc tgagctgggc cgtctctttt cgctcccttg tatcgtccag ttgttttttc
     4501 agctgagtaa gggccttgtt ttctttagtc aatgaattaa tctgctccag cactttatgg
     4561 ataccgcgtt gtaatccggc tatatcaagt tcctcaaacc ccgcatgttc ctggatccgg
     4621 ctgttggcat aaaactgctg ctgcaggcgt ttgcccttat tggtacaccg ctccgttatt
     4681 tcggcggatt gctgtaattc atccgacaac tgatcccttt tcttaaacaa ggcctgcttt
     4741 ttcttttcat tgttccagcc caatacataa cggctgggat cattgcggtc cggacgatca
     4801 tctttctcat gccgctggcc attcttgatc agtccattta atgtaagcgc ccgggtataa
     4861 cggtccagtg tcttctcatc atcaatacag gcataattaa actggcggat cacttgctgt
     4921 tccacccaat tgcccagttt atgatccgga tgaaactcca gtttctcatg caccgtaccg
     4981 gcttccggat gctgcaccag ggcattatcc gttacatgtt cataaacgaa acgggcccgc
     5041 aggttctcgc ggttcacata tttgtttact ttcttgtaat atttatcggg cacgagtaac
     5101 cgggtgctga aagaacgtaa taatttctct acggcaggct gccagtccag ttcttcggta
     5161 cgtacctgca tcagctcccc ggcaaaaggc agttcgtgcg gtacaatatc cagggcctca
     5221 cttaactggt tacggatctg aatcaggtag gaggggatat tgtttttact gaccagcagg
     5281 ttgttcagct ctttctccac gatctctttc tccgccgtag caatatcctt tttccgcttg
     5341 gcggtatatt catcttccgc attcagccgt tgctcggtat tcaacgcaag cagtttccgc
     5401 tgattctctt ttttgatccg cagataagcc gattcatcag gcgcggtggt ctctggtaaa
     5461 tgcagggtgc cgcaccattt agtaaattcc tcgagtctga tcgaggccgt attcttcttc
     5521 tcttcgagtt cttgctttga tttttccagc tgctgcaaac gctgaccggc ctggttctga
     5581 tcgatctggt tcaggatggt tcgttcttct tccgcaagcg tcgccagctc ttttctatgc
     5641 tgcgcgattt tctgtaccag ttctttttcc tgttgccgga tctgttcgga tgcaacacgg
     5701 agcaattcat gttgcgtata ttttttccag atacccgctg tgttcagcag ggtctccagt
     5761 tcttctttct gttgttttag ctgctgaaac tgttcatact ggtcttttac cggttgcagc
     5821 attttcaact gctcttccgc cttttcaata ttacgacggg catccagcag ggtggaaagg
     5881 tgtttcttca gttcctggaa ctggtcttca atattccggg gttccagcat atgggtgcgg
     5941 atgaactcat ccaggttgcc cagcacttta atcccgactg tctggttaaa taaatgcagg
     6001 gcctgtatac tctgcatccc cagtacttcc acgatgcgtt gcgcataacg gctggcggca
     6061 tcaaaccatt ccacaaattt gcggctgcct ttattgtacc gctgatcaat ggtttttttc
     6121 caggcaccac caaggtcaaa aggtttgaaa tcttcttcga tcttcagggc cttgtgcgcc
     6181 acaccgaaat gtttcttcat ttccccattg acaaaatagc gaacctggaa caaggtaact
     6241 acctgttctg cttcattggc aaaattgacc aatatgatgc tgtacgcctc gtctttattc
     6301 tcgcggagat ataaggtctt ggtggaaccg gtactttcac tgctgatggt gccatagcct
     6361 ccgagtacat aactgtcttc ggtacgatcg ccttttttct ccgagccgct gctctggtta
     6421 taaaagcgat aacgcttctc cggtacaatg agggtcagca aggcatccac gaacgtggtc
     6481 ttgccactgc cgttggcccc ggttaacaga ctcgtttccc cgccgggatg aatggcatgg
     6541 atagtatcat caaatgtgcc ccagttgaaa atttccatgt actgaaggcg gaatccgctc
     6601 ttctggttat cggtactaaa tacactcagc tgcatgtgac aatcgtttaa gggttggatt
     6661 ctttttgtaa tttctccttg tgctgtttca gttgctggta aaaactatcc agttcttccg
     6721 catccacccg ggctttgatt atttttttga tacggaaccg ttgttcatcc gcaatctcat
     6781 ggtcatcggt catggccagg aatccgtatt cctcagtctt atcaatcagc cggtcgatct
     6841 ctttcagaaa tttcacccga ctggcatttt ctttaaaaaa cagttccgca tactccttga
     6901 tctcccggcg cttcttcacc agttcgcggt tattgctgtc gccggtttca aactcggcca
     6961 tcatttcacg gagcaatacg agcatgatgc tttcctcata cgtgagtgcc cggcggatga
     7021 tccagcttac actggcatcc tcctcttccg gattcacatg caggagataa gcatagccat
     7081 cttcctc
//
LOCUS       DAOPMW010000148         7069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12090_length_7069_cov_6.736527, whole genome shotgun sequence.
ACCESSION   DAOPMW010000148 DAOPMW010000000
VERSION     DAOPMW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12090_length_7069_cov_6.736527"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..741
                     /gene="aat"
                     /locus_tag="PLU11_08280"
     CDS             91..741
                     /gene="aat"
                     /locus_tag="PLU11_08280"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPN59155.1"
                     /translation="MSLFILDNEIHFPPVHLADPDGLLAIGGDLSPARLLEAYRHGIF
                     PWYEGSPILWWCPNPRFVLFPDELKIARSMKPLLNRQAFEFTTNTAFAAVIHQCKNSF
                     RPGQEGTWITDEVEKAYIKMHEIGYAHSAEVWKNGQLTGGLYGIRLGKVFFGESMFSK
                     ESNASRYAFIKYTEQLIKEGVSLIDCQVHTGYLESLGARMIPGKEFDQLLQNLVER"
     gene            complement(720..1451)
                     /locus_tag="PLU11_08285"
     CDS             complement(720..1451)
                     /locus_tag="PLU11_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPN59156.1"
                     /translation="MKRAINSWFSPSLNKEMPIATYGDYGFALLLVPTAAADYLEYER
                     FQLMEHIASHINGGKVKVFSIDSINNESWMNNHVDPRHKTYRHQQWNNYVYNEVVPFI
                     RHNTSADTPIITCGASFGALHSMNLFLKRPDLINGVIAMSGVYDLTEYSKGHFDEDVY
                     FNSPMHFMPNLNDHAVLEQIRRSHHLHILTGSGPYEDPSSSGRFAKILYDKGLHYELD
                     IWGQEWPHDWNTWKAMLPHYLSTRF"
     gene            1789..2214
                     /locus_tag="PLU11_08290"
     CDS             1789..2214
                     /locus_tag="PLU11_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59157.1"
                     /translation="MKDELIRIMLVDDHPLVRESWKLLLENNPRFEIVADYSNGAEAL
                     ENIPSLKPDILLVDINMAPLDGFAVTKSVNTMMPEIKIIGLSVHNQPKYATRLLQLGA
                     RGFLTKTSSLEEINHGILAVHQGKVYICDEIKRNMPPAG"
     gene            2333..5071
                     /locus_tag="PLU11_08295"
     CDS             2333..5071
                     /locus_tag="PLU11_08295"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPN59158.1"
                     /translation="MELSKNFEHQSAEEKWYRHWLDKNYFHSEPDHREPYTIVIPPPN
                     VTGVLHMGHCLNNTIQDILIRRARMQGKNACWVPGTDHASIATEAKVVHMLRERGIAK
                     SSLSREEFLKYAWEWKEKYGGIILQQLKKMGCSLDWDRTSFTMDPDYYQSVISVFIDL
                     YKKGIIYRGLRMVNWDPASKTALSDEEVIFKEIDSRLYYVKYPLAEDATRFITVATTR
                     PETILGDVAICVNPADERYSSWVGKEVIVPLINRKIPVIADEYVDAEFGTGALKITPA
                     HDKNDNEIGKKHGLKALNTLDEEGKFTAEELADDHPSQLILSYTGTDRFDLRIKMAED
                     IGQLGLLEKTKVYKGQVGTSERTGAIIESRLSLQWFMNMQEFLNRNPETLSAVMNDKI
                     SFHPEKTKNTYSHWLNNIKDWCISRQLWWGQQIPAWYDEAGNVYVAATEEEAQLQAMS
                     YEQRATSQDNSLLATRNPKPVLKQDEDCLDTWFSSWLWPMEVFKGISNPGNRDIEYYY
                     PTTTLVTGQDIIFFWVARMIMAGYEYKKEIPFKDVYFTGMVRDKLGRKMSKQLGNSPD
                     LLEMIEKDGADAVRFGIMITSPAGNDLMWDPSGNEQGRFFINKMWNALKLVKMWEGRQ
                     ADKSAGSDTKTESHFAIDWFDHRLKEVRLQLEEQFTDFRLSESLKTIYSLIWDDFCSW
                     YLEWVKPSFAKATEDKAPQPEPISPYVYEKTVGYFEELMQLLHPFMPFITEEIYHQLK
                     ERTDDLTVKQNPTASQPDQGLLESAALLKEVITAIRDARVKAQLKPKETIKLHIQSEN
                     QAVYTPILSILAKQVNAESTGFTNDPVTGSITIVVQKDKFYLETTSVLDTGAQKQQLQ
                     KDLDYLKGFLVSVDKKLSNERFVQNAKPEVIDIERKKKADAEDKIKVIEESLASLG"
     gene            5132..5761
                     /locus_tag="PLU11_08300"
     CDS             5132..5761
                     /locus_tag="PLU11_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPN59159.1"
                     /translation="MRLFKKYILLLLIGGCFPFLLFSQRNNWEYDLLRTLAEKRIPAG
                     NQFHKGFSKLNRPVCLAVPAGLFITGLLEKDRTLKENALFITGSMVLSSMTTWTLKKI
                     VNRERPAVNDPSFIAVTNELDSGFPSGHTSLAFSMATSLSMKYPRWYVVVPAFAYAGL
                     TGYSRLYLGVHYPTDVVAGALVGSGSAWLTWKLNQWIKREKKKKPAPAL"
     gene            complement(5766..>7069)
                     /locus_tag="PLU11_08305"
     CDS             complement(5766..>7069)
                     /locus_tag="PLU11_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59160.1"
                     /translation="YQGNDEVFALRLDTQKIYRVTEGSMGRYFVNAGDDKLTWSVFSA
                     EGYQLQQQAIDPQLWKPVDMAVTEQLTGQWPAAGVPGTLMHLADTAASRRFASSPYRK
                     GTRLFNFHSWRPYYEDPVFTFSLYGENVLNTLQTELYYLYNENEKTSAVGFSSIYGAG
                     FPYLSAGTELTFNREIPIGSRIRQWNQLDSRVGFTLPVSRTSGTTFKNFSLSSFYVLR
                     NEFNKDFYKDSLGYTSFSYLSHTVSWSEQIQRAVQHIYPRFAYSASASFRHAISRYEA
                     RQFNGTAALYVPGVLSNHNLLLNAAFQERDTLGQVSFGNRFTYSRGYTGRSFTRMWKL
                     SANYHLPLWCPDWGVGNILYIQRIRMNMFYDFTKVYSVDKTQTRDQRSVGGELFADTK
                     WWNQYPLTFGIRVSQLLDRDQYDGFKGTFFEFVMPVSIFPR"
BASE COUNT         2083 a         1611 c         1616 g         1759 t
ORIGIN      
        1 agctatgacg acctgaaacc cttttgcgat gccattacag aaagaggcat caacgcgact
       61 attgaagtag tggcgggata agccctgttc atgtccctat ttatacttga taatgagatt
      121 cattttccgc cggtacacct ggcagatccg gatggtttgt tggctatcgg cggggatctc
      181 tcccctgccc gtttgctcga agcctaccgg catggcattt tcccctggta tgaaggctca
      241 cctattctct ggtggtgccc caatccccgg tttgttctat ttccggatga actaaaaatt
      301 gcccggagta tgaaacccct gctgaacaga caggcctttg agtttacgac caataccgct
      361 tttgctgctg tgattcacca atgtaaaaac agtttccggc ccggacagga aggcacctgg
      421 attactgatg aagtggaaaa agcctatata aaaatgcatg aaatcgggta tgcgcacagt
      481 gccgaggtat ggaagaatgg ccagctgacc ggcggtttgt atggtatccg gttgggtaag
      541 gtattcttcg gggaaagcat gttcagtaag gaaagcaacg cctcccgtta tgcatttatc
      601 aaatacacgg aacaattgat aaaggaagga gtctccctga tcgattgcca ggtacacacc
      661 ggctacctcg agagcctggg cgcccggatg atccccggaa aggagtttga tcaactactt
      721 cagaacctgg tagaaagata atggggtaac atcgccttcc aggtattcca gtcatgcggc
      781 cactcctgcc cccagatatc cagttcataa tgaagccctt tatcatagag aattttcgca
      841 aaccggccac tgctggaagg gtcttcataa ggaccactcc ccgtcagaat atgcagatga
      901 tggctgcggc ggatctgttc caggactgca tgatcattca ggttgggcat aaaatgcatc
      961 ggactgttga aatacacatc ctcatcaaaa tgacctttac tgtattcagt gaggtcatac
     1021 acaccgctca ttgcaatcac gccattgatc aggtccggtc ttttcaaaaa caaattcata
     1081 ctgtgcagcg ccccaaaaga agccccgcag gtaatgatgg gcgtatccgc actggtattg
     1141 tgccggataa aaggcactac ttcattatat acataattgt tccactgctg gtgacgatag
     1201 gttttatgcc ggggatccac atggttattc atccagctct cattgttaat actgtctatt
     1261 gaaaagactt tcaccttccc tccattgata tgactggcga tatgctccat cagctgaaaa
     1321 cgctcatact ccaggtaatc cgccgccgca gtaggaacaa gtaataaggc aaacccataa
     1381 tcaccataag tggcaatggg catttcttta ttcagggagg gactaaacca ggaattgatt
     1441 gctcttttca tataaagctt tttaatcgcc ggaaccggct gaaaaatttc cagcgaaggt
     1501 aattgtaaaa acaactggaa aagacgctga ttttcagttt caaaaggaga gaaaaaaaga
     1561 aaaccgtatt tttccttggt aaataaacaa agtagtatta cttttatttt acagtatttt
     1621 tactgcatat ttgtatacgt aaatttactt attatttata agtagctgat aattaaagat
     1681 aactactaat ctccttccta aagaaatacc agacgtttaa gaaaacatcc ctttctgtgc
     1741 aagacctatt taaacccgaa agggtcaggt ctaaaagctg gttagtatat gaaagatgaa
     1801 cttatccgta tcatgttggt ggatgaccac ccgctggttc gtgaatcctg gaaattatta
     1861 ttggaaaaca atccccgttt cgagatcgtg gctgattatt ccaatggcgc tgaggccctg
     1921 gagaatatcc cttctttaaa accggatatc ctgctggtgg acatcaacat ggctccactt
     1981 gatggttttg cggtgacaaa aagcgtaaac accatgatgc ctgaaattaa aatcatcggg
     2041 ctatcggtac ataaccagcc gaaatacgca acccggttac tccaattagg cgccaggggc
     2101 ttcttaacca aaacttcctc acttgaagaa atcaatcacg gtatattggc agtccaccag
     2161 gggaaagtat atatatgcga tgaaattaaa cgcaatatgc cccctgccgg ataatcgcag
     2221 cgcctctcct tctttctgcc cggttttctc cgcaaatcgg taaatttgcg cccttacacc
     2281 actcactaaa aacagctggt ggatgtaaag gaataattat aaggcagcac gtatggaact
     2341 cagcaaaaac tttgaacatc agtccgcaga agaaaaatgg taccggcatt ggctggacaa
     2401 aaactatttt catagtgaac ccgatcaccg ggaaccctat accattgtaa tcccaccccc
     2461 aaatgtaacc ggtgtactgc atatggggca ttgcctcaac aataccatcc aggacatcct
     2521 gatccgcagg gcccgtatgc agggaaaaaa tgcctgctgg gtaccgggaa ccgaccatgc
     2581 ttccattgcc accgaagcca aagtggttca catgctgcgg gagagaggta ttgccaaatc
     2641 ctctctcagc cgggaagaat tcctgaagta tgcctgggaa tggaaagaaa aatatggcgg
     2701 gatcattctt cagcaattaa aaaaaatggg ctgcagtctc gactgggacc gcacttcctt
     2761 taccatggat cctgattatt accaatcagt gatcagtgta tttattgacc tgtacaaaaa
     2821 aggaattatt taccgcggac tgagaatggt gaactgggat cctgccagta aaacagcatt
     2881 gagtgatgaa gaagtcattt ttaaggaaat tgactcccgg ttgtattatg taaaataccc
     2941 gttggcagaa gatgccaccc gatttattac agtggccact acaagaccgg agacgattct
     3001 cggtgatgtt gccatttgtg tgaatccggc tgacgagcgt tattcatcct gggtcggaaa
     3061 agaagttatc gtacccctca tcaaccggaa aatacccgtt attgccgatg aatatgtgga
     3121 tgcggagttt ggaacagggg ccctaaagat cacgccggcc catgacaaaa atgacaatga
     3181 aatcggaaaa aaacatggcc tgaaagcact gaatacgctg gatgaagagg gcaaattcac
     3241 ggcagaagaa ctggccgatg atcatccatc ccaactcata ctttcctata ctggaacaga
     3301 ccgttttgat ctgagaataa aaatggcgga agatatcggg caactgggtt tgttagagaa
     3361 aacgaaagta tataaaggac aggtgggcac cagtgaacga accggggcga ttatcgagtc
     3421 aagattaagt ctgcaatggt ttatgaacat gcaggaattt cttaaccgta atccggaaac
     3481 cttatcagcc gtaatgaatg ataagatcag ttttcacccg gaaaagacga agaacacata
     3541 cagccattgg ctgaataata taaaagactg gtgtattagc cgtcagttat ggtggggaca
     3601 acagatcccg gcctggtatg atgaagccgg gaatgtgtat gtggccgcaa cagaggaaga
     3661 agctcaactg caagcaatga gttacgagca acgagctacg agccaggaca actctctgct
     3721 cgccactcga aacccgaaac ctgtattaaa gcaggacgaa gactgcctgg acacctggtt
     3781 ttcctcctgg ctctggccta tggaagtgtt caaaggcatc agtaatcccg gcaacaggga
     3841 tattgaatac tattacccta caaccacctt agtcaccggt caggatatta ttttcttttg
     3901 ggtagcacgt atgatcatgg ccggatatga atacaaaaaa gaaataccct ttaaagatgt
     3961 atattttacc ggaatggttc gggataaact aggtcggaaa atgagcaagc aattagggaa
     4021 tagtcctgac ttgctggaaa tgattgaaaa agacggggcg gatgcggttc gctttggcat
     4081 catgatcact tccccggccg gcaatgatct gatgtgggat ccatcaggca atgaacaagg
     4141 tcgtttcttc attaataaaa tgtggaatgc cctgaaactg gtgaaaatgt gggagggcag
     4201 acaggctgat aaatccgccg gatctgatac aaaaacagaa agccatttcg ccattgactg
     4261 gtttgaccat cggttgaagg aagtaagatt gcaactggaa gaacagttta ccgacttccg
     4321 gctgagtgaa tcactcaaga ccatctactc cttgatctgg gatgatttct gttcctggta
     4381 cctggaatgg gtcaagcctt ccttcgctaa agctacggaa gacaaggccc cacagccgga
     4441 acctatcagt ccttatgtgt atgaaaaaac agtaggttat ttcgaagagc tcatgcagtt
     4501 actgcatcct ttcatgccat ttattactga agaaatttat catcagttaa aggaaagaac
     4561 ggatgatctc acggtgaaac aaaatccaac ggcaagtcaa ccggatcaag gattgctgga
     4621 atccgctgct ttgttaaaag aagtgattac tgctataagg gatgctcggg tgaaagccca
     4681 gctgaaacct aaagaaacta tcaagctgca tattcaatcc gaaaaccagg cggtttatac
     4741 gccgatcctt tccatactgg ccaaacaagt aaatgctgaa tccaccggat ttaccaatga
     4801 tccggtaaca ggcagtatta ccattgtagt acagaaagat aaattctacc tggaaaccac
     4861 ttccgtactg gacaccggtg cgcagaaaca acagttgcag aaagacctgg attacctgaa
     4921 aggcttcctg gtatccgtag ataaaaaatt gtcgaatgaa cggtttgtac aaaatgctaa
     4981 accggaagtg atcgatatcg aacggaagaa aaaagcggat gccgaagata agatcaaagt
     5041 aatcgaagaa agcctggctt cactgggttg aaaataaata ctgactttta aaacacttta
     5101 ccaaaactaa agtgtttttt tattttataa aatgcgctta tttaaaaagt atattctgct
     5161 gttgctgatc ggcgggtgct tcccgttttt gctgtttagc cagcggaaca actgggaata
     5221 tgatctgctt cggaccctgg cggaaaaaag aatccctgcc ggcaatcaat ttcataaggg
     5281 gttttcaaaa ttaaaccgcc ctgtctgcct ggcggttccc gccggattat tcattaccgg
     5341 tctgttagaa aaagacagaa cgctgaagga aaatgcactt tttatcaccg ggagtatggt
     5401 attatcatcg atgactacct ggactttgaa aaaaatcgtc aaccgggaaa gaccggccgt
     5461 taatgatcca tcattcattg cagtaaccaa cgaacttgat tccggattcc cttccgggca
     5521 tacctccctg gcattttcaa tggccacttc attgagtatg aaatatccac gctggtatgt
     5581 agtggttccg gcttttgcct atgccggatt aaccggttat tcgcgcttat accttggcgt
     5641 gcactatccc acagatgtag tggcaggggc cctggtgggt agtggcagtg cctggcttac
     5701 ctggaaactc aatcaatgga taaaaaggga gaaaaagaaa aaaccggcgc ctgcccttta
     5761 aagatttacc tggggaaaat actgaccggc atcacaaatt caaagaacgt tcctttaaat
     5821 ccgtcatact gatccctgtc cagtaattgg ctcacccgga taccaaaggt cagcggatac
     5881 tgattccacc atttggtatc ggcaaataat tctccaccca cggatcgctg gtcacgggtt
     5941 tgtgttttat ctacagaata cactttggta aaatcgtaga acatattcat ccggatgcgc
     6001 tggatataaa ggatgttacc caccccccag tccggacacc agagcggcaa atgataattg
     6061 gccgaaagct tccacatcct ggtgaaagat cgcccggtat aaccccttga ataagtaaac
     6121 cggttgccaa aggatacctg cccgagggta tcccgctcct gaaatgcggc attcagcagc
     6181 agattatgat tagataaaac gccaggcaca tacaatgccg cggtaccatt gaattgcctg
     6241 gcctcataac ggctgatggc atgccggaag gaagcggagg cggaataagc aaagcggggg
     6301 taaatatgct gcaccgcccg ctggatctgt tcactccagg atacagtatg cgacaggtaa
     6361 ctgaatgaag tatatcccag tgaatcctta taaaagtcct tattaaattc attccgcagt
     6421 acataaaaac tgctgagtga aaaattctta aacgtcgtgc cactggtgcg actgacagga
     6481 agcgtgaaac cgacacggct atccagctga ttccattgcc tgatgcggct gccgatgggt
     6541 atttcccggt taaaggtcag ttctgtacct gcactgaggt aagggaaacc ggcgccataa
     6601 atactactaa aaccaaccgc actggttttt tcattttcat tatacaggta atataattca
     6661 gtctgcaacg tattcagcac attctctcca tagagtgaaa aagtaaatac cggatcttca
     6721 tagtaaggtc tccagctgtg aaaattgaac aaccgggtcc ctttgcgata cgggctgctg
     6781 gcaaaccggc gggaagctgc agtatctgcc aggtgcatca atgtacccgg cacaccggca
     6841 gccggccatt gtcccgtcag ttgctctgtc accgccatat ccaccggttt ccatagttgc
     6901 ggatcaatcg cctgttgttg caactggtag ccctctgcag aaaacacgga ccaggtcagt
     6961 ttatcatcac cggcattgac aaagtaccgg cccatgcttc cttccgtaac ccggtagatt
     7021 ttttgggtat cgagccgtag ggcaaacact tcatcattgc cctggtaag
//
LOCUS       DAOPMW010000149         6945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12389_length_6945_cov_10.917997, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000149 DAOPMW010000000
VERSION     DAOPMW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12389_length_6945_cov_10.917997"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLU11_08310"
     CDS             <1..219
                     /locus_tag="PLU11_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59161.1"
                     /translation="ASFFRLDNINFGYNVGRILKDKASMRVSASVQNVFVITKYKGLD
                     PENASDGGVDNNIYPRPRIFSLGFNFDF"
     gene            239..1846
                     /locus_tag="PLU11_08315"
     CDS             239..1846
                     /locus_tag="PLU11_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN59162.1"
                     /translation="MRHISINKFILAGAFLVTLASCSKKLDLFPQNDLTNETTYSTAA
                     GYRAVLAKVYAGLATTGNAGPAGASDIQGLDEGSQSPFIRSFFNCQELPTDEAVVSWN
                     DQTIKDFHNLSWSSSDPFLKGMYARPIYNVMVANEYLRESTDDKLAARNITGADATEI
                     QSTRAEVRFLRAFNYWVMMDIFGKSTFIDETNVVGTTLPGEKNRGELFTFIETELKAI
                     ETELKTVKTIEYGRVDQGAAWALLARMYLNAQTYTGTAKWTEAITYAKKVIAGGYSLH
                     TDYRQLFMADNDKRTNEIIYAVNCDGLHTKSYGNTTFFVHAASGDDHNEFGVGGGWYG
                     YRATKGLADRFTDLSGTTDKRAMFTTSAFGTSPAQIAISDVGNFANGLHVNKYRNIRS
                     DGGTVSDVNRDFADIDFPIFRLSEMYLIYAEAVLRGGTGGDLTTATGYINLIRQRAYG
                     NTSGNITSGQLDLPFILDERARELYWEGHRRTDLIRYNQLTTATYLWPWKGGIASGTA
                     VDAKYNIFPIPATNRTANPNLTQNTGY"
     gene            1871..2698
                     /locus_tag="PLU11_08320"
     CDS             1871..2698
                     /locus_tag="PLU11_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HPN59163.1"
                     /translation="MKSILKLLFGSLFLAVAFSSCEKDENKIYYEGGTAPALTASSTA
                     AQVLLIANKNNTALTLNWTNPNYMFTTGVSSQDVTYTLQFDTVGANFTSPKKYERAIA
                     KELNVALTVGELNTALLAMDMVENMPHNIEMRVKASLINSTVPLISNVITIRITPYLD
                     VVFPVPANLYITGAATPGNWMGGGDPELLSQKFTKISSSEFQINSLAITPDVGFLFVP
                     VYGDWGNKYGFTGAGLANNVNGDSFQPNGNDMKSPGVAGNYKIVVSFKTGKYTMTKL"
     gene            2722..4485
                     /locus_tag="PLU11_08325"
     CDS             2722..4485
                     /locus_tag="PLU11_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HPN59164.1"
                     /translation="MKNIFKILALAVTTVVMVTACTKVADLPYYENGNPVTLTANKTA
                     VTATPADSLNAVISFSWSNPEYKQDSSLYKFVLEIDSTGRNFAKKAMKVVTGVSSTTL
                     TGKELNNILLNYGFALGTPYDLDIRVISSYGNNNESYQSNVLKIRVTPYNDPSVLTTV
                     NTSVTCALATASQLSNTFNWSTSFNGYGGTVTYTLQYDSAGKNFVAPVEIPVGASLFT
                     KALTQGEMNETALTSGIPGGTTGKVEYRVKATTAQGAVSYSNVVNVTIASYVPILRFY
                     LPGGYQGSTGNGNDWDPGSAPELIRDLRPAALNQLYYIYLYLPAGAEFKVTQGRSWDV
                     NYGGSGGNLSSGGANFSVATAGYYRISIDRVNMKYDIREGRMGFVGGATGAGWNPPNV
                     FPNYGMGLAANNLFVGLTDFTVDGWKMIDHNDWNNGDISVVNARSYGSTGASGSTLLV
                     NDANMPNITTAGRYRVMWDGRNVDNVKYEMSPATEMRVVGDGMLGVNAWDPGASPQMT
                     YAGNGVWTITLDLVAGKDIKFLAGNAWGAFDYEDNSGGSTATGTARKIKWEGGDNFKT
                     PTVSGSYTITLNEHMQTVTIN"
     gene            4550..5581
                     /locus_tag="PLU11_08330"
     CDS             4550..5581
                     /locus_tag="PLU11_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPN59165.1"
                     /translation="MKKILTFSVLILLLWTLAFSGSCKSDKPAPKPVPVLPFAKGADI
                     GWLTEMEAAGKKFYNAAGTEMDCIALLKSLGMNSIRLRVWVNPSSGWNNAADVLAKAT
                     RAKNAGMRLMIDFHYSDTWADPGQQAIPVAWAAQTLTELQTSVYNHTFSVLESLKSNG
                     IVPEWVQVGNETNNGMLWPIGKASTSMPNFASLVKQGYNAVKAVDPSIKVILHLSNGY
                     DNNLYRWIFDGMISNGVSWDIIGMSLYPEPIDWATRNSQCLANINDMISRYGREVMIT
                     EVGMSWDQADACRSFITDLITKCKAVSGNKVLGVFYWEPQSYGNWQGYTKGAFDNSGK
                     PTIALNAFN"
     gene            5617..>6945
                     /locus_tag="PLU11_08335"
     CDS             5617..>6945
                     /locus_tag="PLU11_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN59166.1"
                     /translation="MKKQELLLLLALITVQAVTAQRQQLNMDAGWKFAFGHAGDPSRD
                     FNFGISTIFSKSGAAPNTAIDPKFDDKKWRSLDLPHDWAVELPFVYKDNFDVQSHGYK
                     PVGGLFPETSIGWYRKHFTVAAADSGQRFQVQFDGVFRDAQFWLNGFYIGNNKSGYVG
                     VAYDVTDYINYHKENVLVVRVDASQYEGWFYEGAGIYRHVWLNKYANAHIASDGVFAL
                     SEQKGNMTSITVETTLENQGLSAAPLSVISYITDRSGKKLAISKEDALQLPVNGRRAV
                     KQYLLVKEARKWSLDDPYLYRIVSVVKQNGKVIDSVKQRFGIRTIEIKPNGLFLNGVH
                     VKLKGTNNHQDHAGVGSALPDYLQYYRISLLKKMGSNAYRTSHNAPTPELLDACDSLG
                     MLVMDEQRLLNSSPEYMDQFERLIKRDRNHASVFIWSIGNEEGWIHTNSYG"
BASE COUNT         1840 a         1672 c         1690 g         1743 t
ORIGIN      
        1 gcttcattct tccgcctgga caatatcaac ttcggataca atgtgggccg catcctgaaa
       61 gacaaagcct ccatgcgggt atccgccagc gtgcagaacg tatttgtgat cacaaaatat
      121 aaaggactgg atcctgaaaa tgcaagcgat ggtggtgtag acaacaatat ctatccccgg
      181 ccgcgtattt tctcacttgg attcaacttt gatttttaat taaaaaaatt attgagttat
      241 gagacatata tcaataaata aattcattct ggcaggtgcc ttcctggtaa ccctggcatc
      301 ctgctcaaaa aagctggatc tgtttccgca aaatgacctg accaacgaaa ccacttattc
      361 caccgccgcc ggttaccgtg cggtactggc taaagtgtat gccggtctgg ccactaccgg
      421 taatgccggt cctgccggtg catcggatat acaaggactg gacgaaggtt cccagtcgcc
      481 ctttatccgt agtttcttca attgccagga actgcccact gatgaggccg tggtttcctg
      541 gaatgaccag accattaagg atttccataa tctcagctgg tccagttcag atccttttct
      601 gaaagggatg tatgcccgcc cgatctataa tgtgatggtg gccaacgaat acctgcgtga
      661 atcaaccgat gataaattag cagcccggaa cattaccgga gcagatgcca ctgaaattca
      721 atccacccgc gctgaagtac gtttcctgcg tgccttcaac tattgggtga tgatggacat
      781 cttcggtaaa tcaaccttca ttgatgaaac caatgtagtg ggtacgactc tgcccggcga
      841 aaagaaccgc ggagaactct tcacttttat tgagacagaa ctgaaggcga ttgaaacaga
      901 actgaaaacc gtgaagacca tcgagtatgg tcgcgtggat cagggtgcgg cctgggcttt
      961 actggcccgg atgtacctca atgcacagac atataccggt acagctaaat ggactgaagc
     1021 aatcacttat gcgaaaaaag tgatcgctgg tggatactca ctgcacaccg actaccgtca
     1081 actctttatg gccgacaatg ataagcgcac caatgagatc atttatgcag tgaattgcga
     1141 tggactgcat accaaatcgt atggtaatac cactttcttt gttcacgccg cttccggtga
     1201 tgatcacaat gaatttggcg taggtggtgg ctggtacggt taccgcgcta ccaaaggtct
     1261 ggccgatcgt tttaccgacc tgagcggtac taccgataaa agagccatgt ttaccacttc
     1321 tgcttttggt acttcccctg cacagatcgc tatttccgat gttggcaact ttgccaacgg
     1381 actccatgta aacaaataca ggaatatccg ttctgatggt ggtactgttt ctgatgtgaa
     1441 ccgtgatttc gcggatatcg atttccctat cttccggctg tctgaaatgt acctgatcta
     1501 tgccgaagcc gtgctgcgtg gcggtaccgg tggcgatctg accactgcaa ccggttatat
     1561 caacctgatc cgtcagcgtg cttatggaaa tacctccggc aatatcacat ccggtcagct
     1621 ggatctgcct ttcatcctgg atgaaagagc ccgtgaatta tactgggaag gtcaccgtcg
     1681 taccgacctg atccgctaca accaactgac caccgccact tacctgtggc catggaaagg
     1741 tggaatagcc agtggtactg ctgtagatgc caagtataat attttcccga ttccggcaac
     1801 caaccggaca gcgaatccta acctgactca aaacaccggt tattaatctt aataataaaa
     1861 ttgctcgatt atgaaaagta ttttgaaact tctgtttggt tctctgttcc tggcagttgc
     1921 tttttcttcc tgcgaaaagg atgagaacaa aatctactat gaaggaggca ccgccccggc
     1981 gttgaccgct tcctccacag cagcacaggt gctcctgatc gccaataaga acaatacggc
     2041 gctgaccctg aactggacca acccgaatta tatgtttacc acaggcgtaa gttcacagga
     2101 cgtgacttat accctgcagt tcgataccgt aggtgctaat ttcaccagcc ccaaaaagta
     2161 cgaaagggca atcgccaaag aactgaatgt ggccctgact gtaggtgaat taaacaccgc
     2221 cttactggcc atggatatgg tggaaaatat gccgcacaat attgaaatgc gggtgaaagc
     2281 ctcgctgatc aatagtacgg tacccctgat ctcaaatgtg atcacgatcc gcatcacgcc
     2341 ttacctggat gtggtattcc cggttccggc taacctgtat atcaccggtg ctgcaactcc
     2401 gggcaactgg atgggcggtg gcgatcctga attgctgagc cagaaattca ccaagatcag
     2461 cagctccgaa ttccagatca acagtctggc cattaccccc gatgtaggtt ttctgtttgt
     2521 accggtgtat ggcgactggg gaaataaata tggctttacc ggtgccggac tggcaaataa
     2581 tgtgaatggc gatagttttc agcccaatgg taatgacatg aaatcacctg gtgtggctgg
     2641 taattacaag attgtggtga gctttaagac cggtaaatac acaatgacca aactataatt
     2701 acggactata aacaacaaac aatgaaaaat atttttaaaa tacttgcact ggctgttacg
     2761 accgtagtta tggttacggc ctgtaccaaa gtagccgacc tgccctacta cgagaacggc
     2821 aacccggtta cgttgacggc caataaaaca gccgttaccg ccacccctgc cgattcactg
     2881 aatgcagtca tttcattcag ctggagcaat ccggagtata agcaggacag ttcactgtat
     2941 aagttcgtcc tggaaatcga ctctaccgga cggaacttcg ccaagaaagc catgaaagtg
     3001 gtgaccggcg tttcttccac tacacttacc ggtaaagaac tcaataatat cctgctgaac
     3061 tatggatttg cccttggcac accctatgac ctggatatcc gtgtgatttc ttcttacggc
     3121 aataacaatg aaagctatca gtctaatgtg ttgaaaatcc gggtgacgcc ttataacgac
     3181 ccgtccgtgc tgactacagt gaataccagt gttacctgtg ccctggccac cgcttcccag
     3241 ttatccaata cctttaactg gtccacttcc ttcaacggat atggcggtac ggtaacctat
     3301 actttacaat acgattcagc tggcaaaaat tttgtagctc ctgttgaaat accggttgga
     3361 gccagtctgt ttacaaaagc actgacccag ggtgaaatga atgaaaccgc gctgacttcc
     3421 ggtatacccg gaggcacaac cggtaaagtg gaataccggg ttaaagcaac caccgctcaa
     3481 ggtgccgttt cttattccaa tgtagtgaat gtaaccattg cgagttatgt accgatcctg
     3541 cgcttctatc tgccaggagg ttatcagggt tcaaccggaa acggaaatga ctgggatcca
     3601 ggttccgctc cggaattgat ccgtgatctt cgtccggctg ccttgaatca gctctattat
     3661 atttacctgt atctgcctgc cggagcagaa ttcaaagtaa cgcagggccg ctcatgggat
     3721 gtgaactatg gtggttctgg cggtaactta tcatctggtg gcgcaaactt cagcgtggct
     3781 acagccggtt actaccggat ctccattgac cgcgtaaata tgaagtatga tatccgcgaa
     3841 ggtcgtatgg gctttgtagg tggtgccacc ggcgctggct ggaatccgcc gaatgtattc
     3901 ccgaactatg gtatgggact ggccgctaat aatctctttg tgggattaac tgacttcacc
     3961 gtagatggtt ggaaaatgat cgaccacaat gactggaata acggtgatat cagtgtggta
     4021 aatgcaagaa gctacggatc aaccggagcc agtggaagca cattactcgt gaatgatgcc
     4081 aatatgccaa atataaccac tgctggtcgt taccgggtaa tgtgggatgg acgtaatgta
     4141 gataatgtga aatatgaaat gtctcctgcc acagaaatgc gcgtggttgg tgatggtatg
     4201 ctgggcgtaa atgcctggga tcccggagcc agtccgcaaa tgacctatgc tggtaacggg
     4261 gtatggacca ttacccttga cctggtagct ggtaaggata tcaagttcct ggcaggaaat
     4321 gcctggggtg cttttgatta tgaggacaac agcggcggca gcacagccac cggtacagcc
     4381 cgcaagatca aatgggaagg tggtgacaac ttcaagacac cgaccgtgtc cggaagctat
     4441 accattaccc tgaatgagca tatgcaaacc gtgaccatca actaagtcat gaaaattaaa
     4501 tagtataaaa cgccgccttt tttaagggcg gcgttttaat tttaggtata tgaaaaagat
     4561 cctgaccttt tccgtgctta tcctcctctt atggacgctt gccttttccg gttcctgtaa
     4621 atcagataag ccggctccaa aacccgttcc tgtacttccc tttgccaaag gagcggatat
     4681 tggctggctg accgaaatgg aagccgccgg taaaaagttt tataatgccg cgggtacgga
     4741 aatggattgc attgccctgt taaaaagcct gggcatgaac tccatccggt tgcgggtttg
     4801 ggtaaatccc tccagtggct ggaataatgc cgctgatgta ctggccaaag ccacccgtgc
     4861 taaaaatgcc ggtatgcgcc tcatgatcga ctttcattac agcgatacct gggctgaccc
     4921 cggacaacag gccattcccg ttgcctgggc cgcacagacc ctgaccgaac ttcaaacatc
     4981 cgtatataac catacattca gtgtgctcga aagtcttaaa agtaatggca tcgtgcctga
     5041 atgggtacag gtaggcaatg agaccaataa tgggatgttg tggccaatcg gcaaggcatc
     5101 caccagtatg cccaatttcg cctccctggt taaacaaggt tacaatgcgg tgaaagccgt
     5161 ggatccttct attaaagtaa tcctgcattt atccaacggg tatgacaata acctgtaccg
     5221 ctggatcttt gacggcatga tcagtaatgg ggtcagctgg gatatcatcg gcatgtcgct
     5281 gtacccggag ccgattgact gggccacccg caacagtcag tgcctggcca atatcaatga
     5341 tatgatcagc cgctatggcc gcgaagtgat gatcaccgaa gtgggcatga gctgggatca
     5401 ggctgatgcg tgcaggtctt tcatcactga tctgattact aaatgcaaag ccgtatcggg
     5461 caataaagtg ctgggtgtct tttactggga gccccagagc tatggcaact ggcagggcta
     5521 taccaagggc gcatttgata attcaggaaa gccaacgatt gcgctaaatg cctttaacta
     5581 acaactgtac ataggattgt ttactaactt gctcttatga aaaagcagga actcctgtta
     5641 ttgcttgcac tgattacagt acaggcagta actgcccagc gtcagcaact gaatatggat
     5701 gccggctgga aattcgcttt tggacatgcg ggtgatcctt cccgtgattt taacttcggc
     5761 atcagtacga tcttctccaa atcaggcgcc gctcccaata ccgccatcga tccgaagttt
     5821 gatgataaga aatggcgcag tctcgatctg ccgcacgact gggctgttga acttcccttc
     5881 gtatacaaag ataattttga tgtgcagtcg catggctata aacctgtcgg cggcttattt
     5941 cctgaaacca gtattggctg gtaccgcaaa cattttaccg tagccgctgc cgattccggt
     6001 cagcgttttc aagtacagtt tgatggcgtg ttcagggatg cgcagttctg gctcaatgga
     6061 ttttatatag gaaataacaa gagcggttat gtgggcgtgg cctatgatgt aaccgattat
     6121 atcaattacc ataaagagaa tgtactcgtg gtccgtgtgg atgcctccca gtacgaaggc
     6181 tggttttatg aaggtgccgg tatttaccgc catgtatggc tgaataaata tgcgaatgct
     6241 catatagcgt ccgatggtgt atttgccctg tctgagcaaa aagggaatat gaccagtatc
     6301 acggtggaaa caaccctgga aaatcagggg ctgtctgctg cgccgctctc tgtgatcagc
     6361 tatataacgg accgtagtgg aaagaaactg gccatcagca aggaagatgc ccttcagctg
     6421 ccggtgaatg gccgcagggc ggtcaaacag tatctgctgg tgaaagaggc ccgtaagtgg
     6481 tcgctcgatg atccctatct ctaccggatc gtatcggtgg tgaaacaaaa cgggaaagta
     6541 atcgacagtg taaaacagcg ctttggtatc cgcacgatcg agatcaaacc aaacggtctt
     6601 tttctcaatg gggttcatgt gaaactcaaa ggcaccaata atcaccagga tcatgcgggg
     6661 gtgggcagtg ccttacccga ttacctgcag tattaccgca tcagcctgtt gaaaaaaatg
     6721 ggcagcaatg cctatcgcac cagtcataat gcacccaccc cggagttact ggacgcctgt
     6781 gacagtctcg gtatgctggt gatggacgag caacgcctgc tcaacagcag tcctgaatat
     6841 atggatcagt ttgaacggct gatcaaacgt gaccgcaatc atgccagtgt gtttatctgg
     6901 tccatcggca atgaagaagg ctggattcat accaatagtt atggc
//
LOCUS       DAOPMW010000150         6925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12436_length_6925_cov_13.491703, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000150 DAOPMW010000000
VERSION     DAOPMW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12436_length_6925_cov_13.491703"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLU11_08340"
     CDS             complement(<1..527)
                     /locus_tag="PLU11_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59167.1"
                     /translation="MKKIMISFVMLAVLSCTDEKKDAPAADAAGTNTDTPAGLPYTAT
                     YSSNWNSNVSDADLKTVLQSYKDWADGNMKGLSDAMADTVDIDLTSGKSMHMSHDSLM
                     TMWGKSRDSLSSVVISMDAWHKMYSVDKKDEVVVTWYKEVDTYKSGKADSGYFHDINM
                     LKKGKIVWFSQYKRP"
     gene            1121..2821
                     /locus_tag="PLU11_08345"
     CDS             1121..2821
                     /locus_tag="PLU11_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HPN59168.1"
                     /translation="MQLLSSTRLLTAGLVLLTTTLLIVLYVSVYQSKKVRDTSEQVNA
                     TQLIIEHTQKLVMTTLDNETGARGYIITGNEDFLDSLYTAGLNLQTKIVQIKDLLGRN
                     REQQQLLDSLVKYIQLRILFSKDLIRAKREQPPDISRRMVENGAGKEHMDHIRMYGNK
                     MEKSVIAMLALRKRKNEQSIQRLNYILYSVLAVVFIASLITIRKLHRDIRNREAGEKK
                     FRALLDAAPDATIIADEKGIIRMINKQAEDLFDYTRKEMTGQPVEILVPAALHAAHVH
                     HRNTFMKTARLRAMGAGLDLFAVKKDSSQFPVEISLSPIHTDEGLMVIASVRDITHRK
                     HLESALRRSNAEMEAFTYSVSHDLRAPLRGIVGFTTILEEDYGDILDEEGQRITGIIK
                     TNTLKMGHLIDDLLAFSRLGRLELSKADVNMSDLVWDIINETIRPEIKKKVQWTVGQL
                     PVVSADLNTLRQVWVNLLSNAVKYSSKKEEPVISVGHNSEDGEQIFFVKDNGVGFDNK
                     YKEKLFKVFQRLHNEEDFEGTGVGLALVEKIIFRHGGRVWAEGVVGEGAVFYFSLPGV
                     "
     gene            2930..3379
                     /locus_tag="PLU11_08350"
     CDS             2930..3379
                     /locus_tag="PLU11_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN59169.1"
                     /translation="MPHSNTVEILLAEDNQNDAELTIRELRKHNMANNLVHVKDGEEA
                     LDFIFGNGQYTSRKDAIHFPKVILLDIQMPKVNGMEVLQIIKNDPRTKAIPVVILTSS
                     KEHPDVQRCYELGANSYIVKPVNFESFAESIRHLGFYWLLLNQPPAY"
     gene            3389..3949
                     /locus_tag="PLU11_08355"
     CDS             3389..3949
                     /locus_tag="PLU11_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN59170.1"
                     /translation="MKKELKILMLEDSPTDAELIQRFLQKEYADSRFKLAVTRDGFLK
                     ALEEFEPDLILADNSLPQFDATEALRIVRERGSQIPFIMVSGTTKEEYAVQIIKSGAD
                     DYILKESLTRLPAAIASAFRHREAEGEKQEVLQALEQSEEKYRTIVDRISDGFMILDK
                     QWQVIYANLKADDIFGKPRGFRKGKN"
     gene            3970..5874
                     /locus_tag="PLU11_08360"
     CDS             3970..5874
                     /locus_tag="PLU11_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPN59171.1"
                     /translation="MGRPFFNAYTEALETKKQVNVEEYSVVAEHWIRAVLYPSEKGVA
                     VFFHDITDQRKAELEVKKIEERYIAVISRISEGFIVLDAEWKILFVNPTTEQFFNRPA
                     DLLIGKNIKDDFPKVIQGTPFFGTYQQAVFSGENQYLDAYSELLGRWVQANYYPSPEG
                     VAIFFRDVTNQKIAEEQKRITQLELRRSEDNYKDLVERISDGFMVIDRRWVVTYINST
                     AEKLLLRQPGYLLGKKMKDEFPGALDRPFLVAFDEALRTGQNKYIEEYFIPTNRWFQA
                     SIYPSVDGVAVFFRDITEQRKAEEEVRKSEEKYRVFIQRITDAFISLDRNWCYTYLNK
                     QAGEMIRRKPEELIGKNVWAVFPDAVHTGTFKAFSLAMEEQRYVTNVDYYAPLDLWQE
                     NHIYPSPDGISVFIRNISEKKKLELELSDQQKKEQQKMIAVAIEAQEKERNAIAGELH
                     DNLNQILVGTRVMLSVLKDSPGKLDQVLDTCMNNLGMAILENRKIAHELITPDLATEN
                     LLEQLGRLCDTMLRPAGIDPFIQKSDFSEDKLTSQQKLAVYRIAQEQCTNIVKYARAK
                     SVIFALSTPESQFVLRITDDGVGAVPGKTNDGIGLQNIRNRVAVFGGTVQINTKSGEG
                     FELEINMPAG"
     gene            6043..>6925
                     /locus_tag="PLU11_08365"
     CDS             6043..>6925
                     /locus_tag="PLU11_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59172.1"
                     /translation="MPDLNHKEEVLRRSLEQTADCWKQFPHLTDTDFVADFTQCLHWR
                     YEGHPSEEAMNTYLKEFEIPQAFLFDILANRFPLVLSSQTLLISTICTAAADHKQITL
                     LDLGCGRGEQLVRILHALNELPELRKVTVIGTEILNSSLEFCASYLVSVSHRFRFNLE
                     FHALSGPVENMTRESVDDLIPEQSGALLINASLLMHHVQQVEKRRAIFELLYSLQPVL
                     LTMVEPDTDCFTDDADQRILNAYEHFGSLYTYISTLPVNASERKGLKQFFRNELYDTL
                     VLADTHRFEKYIRGSEWI"
BASE COUNT         1985 a         1442 c         1706 g         1792 t
ORIGIN      
        1 atcggcctct tatactgact aaaccacaca atcttcccct tcttcagcat attgatatca
       61 tggaaatagc ctgaatcggc tttgccggat ttgtaggtgt ctacttcttt gtaccaggtg
      121 accacgactt catctttctt atcaacggaa tacattttat gccaggcatc catgctgatg
      181 actacggaac tgagactgtc gcggctcttg ccccacatgg tcatcaggct gtcgtgactc
      241 atgtgcatgg attttccact ggtaaggtca atatctacgg tatcggccat ggcatcgctg
      301 aggcctttca tattgccatc ggcccagtct ttgtaggatt gtaaaacggt tttgagatcg
      361 gcatcggaga cattgctatt ccagttgctg ctatacgtag cggtgtaggg caaaccagcc
      421 ggggtgtctg tgttagtgcc ggcagcatct gcagcaggag catctttctt ttcatccgtg
      481 caggacaaaa cagcaagcat tacgaaagag atcatgatct ttttcattat cgttggtttt
      541 tggtaacgtg gctatgcggg cggaaaaggg gggggcggag tggggggatt gttgttaagg
      601 tagggcaatt tggtgacagc tggaagttta gttttttatg gaggtttggc gagtcggcag
      661 tcggcagtcg ggagtcagca gtcaacagtc ggcagtcagc agtcggcagt caacagcccg
      721 tactgagggc aatcggttgg cggatagttg atagtgagtg aaaggctgtt acttacaact
      781 taccacttaa aacttacaac ttttacttta tcatatgtag taaactactg cttaatcaca
      841 tagcgtggtc agtgtttaaa caactaaatt tcatttacag attaattata ttaatctcaa
      901 aatgattact gatttcgtaa acaagcagtc attattttag ttagattact ataagtagat
      961 ttttttcagg gagcttaaac aggataaaac accaaccagc tgccggttat tttaaggcag
     1021 cgaaacaaaa aaccaacggt cggcaatgct atgtattgtt gttccgtatt aaacacgtag
     1081 tcaagtggtt gccccggcaa ctatgcgaac aacgattggc atgcaactat tatcctccac
     1141 tcgtctgctt actgcaggtc tggttctgtt aaccacgacc cttttaatcg tattatacgt
     1201 ttcggtttat cagtcaaaga aagtcaggga tacatctgaa caggtgaatg ctacccagtt
     1261 gatcattgaa cacacccaga aactggtaat gacaaccctg gataatgaaa caggggcccg
     1321 aggctatatt ataaccggta atgaggattt cctggattct ttatataccg ctggtcttaa
     1381 tctgcaaacc aagattgtgc agattaaaga tctgctgggc cgaaaccggg aacaacagca
     1441 actactcgac agcctggtta aatatataca gttaaggatt ctcttttcaa aagatctgat
     1501 tagggctaag cgggaacagc cgcccgatat atcgaggaga atggttgaaa acggagcagg
     1561 gaaagaacat atggatcaca tccgtatgta tggaaataaa atggagaagt cggtcatcgc
     1621 tatgctggca ctccgtaaaa ggaaaaatga gcaaagtata cagcggctga attatattct
     1681 ctattcagta ctggctgttg tctttatagc aagcctgatc acgatccgga aattacaccg
     1741 ggatattcgt aaccgggaag ccggcgaaaa aaaattcagg gccctcctgg atgcagctcc
     1801 cgatgcaacg atcattgccg atgaaaaagg aatcatccgg atgatcaaca aacaagccga
     1861 agatttattt gactatacac gaaaggaaat gaccgggcag ccagtggaaa tactggtgcc
     1921 tgctgccctg catgccgcac atgtgcatca ccgcaataca tttatgaaaa cggcccggct
     1981 gcgggccatg ggagcaggat tggatttatt cgcggttaaa aaagacagct cccagttccc
     2041 ggtggagatc agtctctccc cgatacatac cgatgaaggc ctgatggtga ttgcctccgt
     2101 ccgggatatc acccatcgaa aacatctcga atcggccctg cgccgcagca atgccgaaat
     2161 ggaagccttt acttattctg tctcccatga cctgcgggcc cccctgcgcg gtatcgttgg
     2221 ttttaccacc atactcgaag aagattatgg ggatatactg gatgaagagg gacaacggat
     2281 caccggtatt atcaaaacca atacgcttaa aatgggtcac ctgattgatg acctgctggc
     2341 tttctccaga ctgggcagac tggaattatc caaagccgat gtaaatatgt ctgacctggt
     2401 gtgggatatc atcaatgaaa caatccggcc agaaataaaa aagaaagtac aatggacggt
     2461 tggccagcta ccagtggtca gtgccgatct gaataccctg cgacaggtat gggtgaacct
     2521 gctgtccaat gcggtgaaat attcttccaa aaaggaggag ccggtgatca gcgtgggaca
     2581 taattcagag gacggtgaac aaatcttctt tgtaaaagat aatggagtag gatttgataa
     2641 taaatacaaa gaaaaactat tcaaggtttt tcaacggctc cataatgaag aagattttga
     2701 aggaacaggt gtgggactgg cgctggtgga gaagatcatc ttccggcatg ggggccgggt
     2761 gtgggctgaa ggagtggtgg gagagggagc ggtgttttat ttttcgttgc caggagtatg
     2821 agagaagtcg ggagtcagga gtcgggagtc aggagtcggg agtaaacagc cccttctaag
     2881 aaaacaacct tgtaaaccta taaacctatc aacctttaaa ccttaccata tgccacacag
     2941 caataccgtc gaaatcctgc tcgccgaaga caatcaaaac gatgccgaac tgacgatccg
     3001 tgaattgcgg aagcataata tggccaataa cctggtacat gtaaaagacg gggaagaggc
     3061 ccttgatttt attttcggta atgggcagta tacttcccgc aaggatgcta ttcactttcc
     3121 caaagtcatt ttgctggata ttcagatgcc caaagtaaac ggaatggaag tgctgcagat
     3181 catcaagaac gatccccgta cgaaggccat accggtagtc atcctcactt cctccaaaga
     3241 acaccccgat gtgcaacgct gttatgaact gggcgcaaac agctatatcg ttaaaccggt
     3301 gaattttgaa agttttgctg aatccattcg tcacctgggc ttttactggt tgctgttgaa
     3361 tcagccaccg gcctattaaa cttattttat gaagaaggag ttgaaaattc tgatgctgga
     3421 agacagcccg actgatgcag aactgattca gcgtttttta cagaaggagt atgcagacag
     3481 ccgctttaaa ctggcagtca cccgcgatgg attcctgaaa gcgctggagg agtttgagcc
     3541 tgatctgata ctggcagata attcattgcc ccagtttgat gccacagaag cgctgcgtat
     3601 tgtccgggaa cggggaagcc agataccatt tattatggtc tccggtacca ccaaggaaga
     3661 atatgcggtg cagattatca aatcgggtgc ggatgattat attttaaaag aaagcctgac
     3721 ccgtttaccg gcagccattg cctcagcctt tcgtcaccgg gaagcagaag gtgaaaaaca
     3781 agaggtcctg caggcactgg agcaaagtga agagaaatac cgcacaattg ttgaccggat
     3841 ctcggatggc tttatgattc tggataaaca atggcaggtt atttatgcca acctgaaagc
     3901 agacgatatt ttcggtaaac cccgggggtt caggaaggga aaaaactgaa tcttgagttt
     3961 ccgcaggcag tgggccgtcc cttttttaat gcttatacag aggcgctgga aacaaaaaaa
     4021 caggtaaatg tggaagagta ttctgtcgtt gcggagcatt ggatcagggc ggtattatat
     4081 ccttcagaaa aaggggtggc cgttttcttt catgacatta ccgatcaacg gaaagcagaa
     4141 ctggaagtta aaaaaattga agaaagatac attgctgtca tcagccgtat ttctgagggc
     4201 tttatcgtac ttgatgctga atggaaaatt ctgtttgtca atccgaccac ggagcagttt
     4261 tttaaccggc cggctgattt actgatcggg aaaaatataa aggatgattt tcccaaagtt
     4321 attcagggta ccccgttttt tggaacctat caacaagcgg tattcagcgg cgaaaatcaa
     4381 tacctggatg cttattctga attattaggc cgttgggtac aggctaatta ttatccttca
     4441 ccggaagggg tggctatttt tttccgggat gtcaccaacc agaaaatagc ggaagagcaa
     4501 aaaagaatta cccaactgga acttcggaga agtgaggata attacaaaga cctggttgaa
     4561 cggatttcgg atggatttat ggtcatagac agaagatggg tggttacgta tataaattcc
     4621 acggcagaaa aactattact aagacagcca ggatatctct taggtaaaaa gatgaaagat
     4681 gaatttccag gcgcactcga tcgtccgttc ctggtagcat ttgatgaggc tttgcgcacg
     4741 ggacaaaata aatatattga ggagtacttc atcccgacca atcgctggtt tcaggccagt
     4801 atatatccat cggtcgatgg ggtagctgtt ttcttccggg atatcacgga acaacggaaa
     4861 gccgaagagg aagtccggaa aagcgaagaa aaataccggg tgtttataca acggattacc
     4921 gatgctttta tctccctgga ccggaactgg tgctatactt acctgaataa gcaggccggt
     4981 gaaatgatca ggcgtaaacc ggaagaactg attggaaaaa atgtttgggc ggtgtttccg
     5041 gatgcggtcc atacaggtac gttcaaagcc ttcagtctgg cgatggaaga acagcggtat
     5101 gtaaccaatg tggattatta tgcgccactg gacctctggc aggaaaatca tatttatcct
     5161 tcccccgatg gcatatctgt ttttatacgg aatatttcag aaaagaaaaa actggaactc
     5221 gagttaagtg accagcagaa aaaagaacaa cagaaaatga ttgccgtggc gattgaggca
     5281 caggagaaag aaagaaatgc cattgcaggt gaactgcatg ataatctcaa ccagatcctg
     5341 gtgggtaccc gggtgatgtt atcggtgttg aaagatagtc cgggaaaatt ggatcaggta
     5401 ctggatacat gtatgaataa cctggggatg gctattcttg aaaatcgtaa aatagcgcat
     5461 gaactcatca cccctgacct ggcaacagaa aacctgctcg agcagctcgg gcgtttatgt
     5521 gatacaatgt tacgcccggc aggtatcgat ccatttattc aaaaatctga ttttagtgaa
     5581 gacaaactga ccagccagca aaaactggcg gtgtaccgga ttgcgcagga acagtgtacc
     5641 aacatagtaa aatatgccag ggctaaatca gtaatatttg ctttgtccac accggaaagc
     5701 cagtttgtcc tccggattac ggacgatggg gtgggtgcag tacccggtaa aacaaacgat
     5761 ggaatcggcc tgcagaatat cagaaaccgg gtagctgttt ttggtggtac ggtacagatt
     5821 aatacgaaat ccggagaggg ttttgaattg gaaattaata tgccggcggg ataactgttt
     5881 ttttgaagtt tgacaatttc gacctttgat gtcacttcct gctagttata cataagccta
     5941 ttcacgtatt ttaattggca tatcctcaaa tcattataaa tagctgaact atttgattat
     6001 tcttgtcgtt aacccggata ataacttagt caaaacacat tcttgcctga tttaaaccat
     6061 aaagaagaag ttcttagaag atcgttggaa cagacggcgg actgctggaa gcaatttccg
     6121 catctgacag atactgattt tgtggccgac tttacccaat gcctgcactg gcggtatgaa
     6181 gggcatcctt ctgaggaggc gatgaatacg tacctgaagg agtttgaaat tccgcaggct
     6241 ttcttgtttg atattctggc gaatcgtttt ccattagtat tatcctcaca aacactgctg
     6301 atcagtacta tctgtacagc cgccgccgat cataagcaga tcaccctgct cgatcttggt
     6361 tgtggtcgcg gtgagcaact ggtccgcatc ctccatgcac tgaatgaatt acctgaatta
     6421 agaaaagtga cggtgatcgg gacggagata ctgaacagtt cacttgagtt ctgcgccagc
     6481 tatttggttt cggtgagtca ccgctttcgt tttaacctgg agtttcatgc cctgtccgga
     6541 cctgtggaga atatgacccg tgaatctgtt gatgatttga tacctgaaca atccggggcc
     6601 ctgctgatca atgcttcctt gctgatgcat catgtgcagc aggtagaaaa gcgccgggcc
     6661 atttttgaat tactctattc ccttcaaccg gtcctgctga ctatggtgga accagatacg
     6721 gattgtttta ccgatgatgc ggatcagcgc atcctgaatg cttacgaaca tttcggcagc
     6781 ttgtatacat atatcagcac ccttccggtg aatgcgtcag aacgaaaggg attgaaacag
     6841 tttttccgga atgaacttta cgataccctg gtgctggccg atacccaccg gtttgagaaa
     6901 tatatccggg gcagcgaatg gatca
//
LOCUS       DAOPMW010000151         6913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12476_length_6913_cov_6.306212, whole genome shotgun sequence.
ACCESSION   DAOPMW010000151 DAOPMW010000000
VERSION     DAOPMW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12476_length_6913_cov_6.306212"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /gene="ccsA"
                     /locus_tag="PLU11_08370"
     CDS             <1..2065
                     /gene="ccsA"
                     /locus_tag="PLU11_08370"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPN59173.1"
                     /translation="LSLVQFALGTMVAGIYIFGWKMGSNPFILLRDSGIFDNAPGMHV
                     NFDLSQPVRPDYMNSIKDGNDLNPLLQNYWMVIHPPVLFMGFASTTIPFAFAMAGLWK
                     KKFGEWTKPALPWALFSAAVFGVGIMMGGQWAYESLTFGGYWAWDPVENASLVPWMIM
                     VSGIHTLLIFRHSGHSLKATYLFLILSFAFVLYSTFLTRSGILGEASVHAFTDLGMNV
                     QLLTFLLGFVIPPLAFFLYRLKEIPSVKQEENISSREFWMFIGALVFFLSAIVIIFQT
                     STPVLNKVLGTTIAPPDDAEFAYNSIQVYVAIIIALLTAVSQYLKYKDTSRSYFWKKM
                     LIPAMITLFLGGLFVIFGEINYDKKGPVFQGALWLAVICSIYTIVANAAYIWLGIKGS
                     LKLSGGSIAHVGFGMVLLGILISSSNKEVISNNIGGIPAPLAEGENPRENLTLVKDMT
                     ANMGRYSLTYLGDSAHPKKQLWFYKIRVKSIDGKEDFVLSPNAFVNYKGNEGLMANPD
                     SRHYWDHDVFTYITSIPNPEAKKDTASFKPFVRKAGDTIFYSKGFITLNTPIRRDSVP
                     QELFGKGGYLTELPMVVHAKTGSNYTLTSRLAIAQNGIFALPDTLHAEGLIIQLQSVN
                     PDNTVEVGIKESDALLKYVTLKAYRFPFIRLLWYGVYITAIGILISMVRRIQLNRKKK
                     AGEEI"
     gene            2143..2853
                     /locus_tag="PLU11_08375"
     CDS             2143..2853
                     /locus_tag="PLU11_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPN59174.1"
                     /translation="MRARFSHTFTFPLLLVFLLAKVTSVSAQVVLPEPAVDSTDWALE
                     YMKEQRKKWGPNDTIILPAIWYQAEIVNYKEMEMAWVSNLSPEKLQKYIQEWTRLRNA
                     VYVTYPYARIAGGTINEINTKLTGITSKKERKTVIKSREKQLKSEFSEPLSNLSVYQG
                     KILMKLINRQTGNNCYEIIKEYKGGFNARFYQTIAFFVGGNLKQEWDLKENKMDRDIE
                     NIVREIDGSWYGNQYRAR"
     gene            2926..3663
                     /gene="bshB1"
                     /locus_tag="PLU11_08380"
     CDS             2926..3663
                     /gene="bshB1"
                     /locus_tag="PLU11_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HPN59175.1"
                     /translation="MKVDILAIGVHPDDVELGCSGTLINEIKNGKKVAILDLTQGELG
                     TRGTIETRYEEAANAAMIMGVHARENLKMRDGFFRNDEAHQLQLISAIRKYQPSVVIG
                     NILKDRHPDHGRAGSLITDSCFLSGLAKIETTDAAGNDQVKWRPAYVLQYMQDWYHEP
                     DFLVDISEVFAQRMKSIEAYTTQFHNGNAPQEGPQTYISKPGFLEAVVARARMFGKRI
                     GVDYAEGFLSEKKLGLRNLDALIQNET"
     gene            3775..4839
                     /locus_tag="PLU11_08385"
     CDS             3775..4839
                     /locus_tag="PLU11_08385"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPN59176.1"
                     /translation="MNFPKFSGNAQTFHAELKKRINEYFHEAGKSYTGNFQLYFKAVV
                     LVLAYIAVYTHLVFYTPATWLALTECVVLGGLTAAIGFNIMHDGMHGSFSRKKWVNYM
                     AGLSLNFLGANNFMWKTKHNIIHHTYTNIEGLDDDIEARPLLRLCDTQKYYKIHRFQH
                     WYFWAAYSLLYIWWVFVTDYKKYFRKKIGEVPLKPMSAWDHISFWGFKVLNIFIFVVI
                     PIAQLGFTSWLIGYLVFGLFAGFVLSIVFQLAHTVEDTHFPHADDHNRMEDEWAIHQL
                     KTTANFATNNKVISWLVGGLNFQVEHHLFPKISHVHYPAISKIIKKACDDFGVTYIEY
                     PKMRLALASHVHHLKNLSRK"
     gene            5103..6146
                     /locus_tag="PLU11_08390"
     CDS             5103..6146
                     /locus_tag="PLU11_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HPN59177.1"
                     /translation="MVDLSTYDPNSVSNPNNNIFGLPSDEESARLIILPVPWEVTVSY
                     GAGTARAPEAILKASYQVDLFDPEMPDAWKQGFYMRPVDRKILMKSDYLRKEAELYID
                     YISKGDDVNANQFMCKTLKEVNEGGVFLTEWMHAQSKALLDQDKLVGILGGDHSVPLG
                     FMKALGEKHGEFGILQIDAHCDLRDNYEGFKHSHASIMFNALKEVTQLQRVVQVGLRD
                     YSQVEWDYIKNSGYKVIAYFDREIKERQFCGDTWKHIAEEMISQLPDKVYISFDIDGL
                     DRKLCPHTGTPVPGGFEMEEVYYMLKLLRQSGKKIIGFDLCEVGVSDSDWNANVGARV
                     LFKLCNLLVTSNS"
     gene            6152..6709
                     /locus_tag="PLU11_08395"
     CDS             6152..6709
                     /locus_tag="PLU11_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59178.1"
                     /translation="MKVAEDYVLSLPNTKAKFYDTMGWLLIIIHVTAFSTLAYYESNP
                     TQRWYALLGILLIAIPLIFELVIRKSHQKSTAIQSALTSGTLVWLLFLHYYTIGFISL
                     ALVLLFYIARRDLKVLVNKERIEYPSFPPKKIKWVDLNNIILKDGLLTIDFKNNKIIQ
                     QLIDENNLVDENEFNNYCRDRLAKG"
BASE COUNT         1888 a         1581 c         1569 g         1875 t
ORIGIN      
        1 gctcagcctg gtgcagttcg cccttggcac gatggtggcc ggtatctata ttttcggctg
       61 gaaaatggga tccaatcctt ttattctttt acgtgacagc gggatttttg acaacgcacc
      121 cggcatgcat gtgaactttg acttgtctca accggtacgt ccggattata tgaattccat
      181 taaagatgga aatgacctga atcccctgct gcagaattac tggatggtga tccatccgcc
      241 agtactcttt atgggattcg cttccactac cattccattt gcatttgcga tggccggtct
      301 ctggaaaaag aaattcggcg agtggaccaa acccgccctg ccctgggcct tgttttctgc
      361 agcggtgttt ggagtaggga ttatgatggg cggacaatgg gcatacgaat ctctcacttt
      421 cggtggatat tgggcctggg atcctgtgga aaatgcatcc ctggttccgt ggatgatcat
      481 ggtatccgga atacacactt tgctgatctt ccggcatagc ggacattcac tcaaagccac
      541 ttacttattt ttaattttgt catttgcttt tgtactctat tccactttcc tgactcgtag
      601 cgggatactg ggtgaagcct ctgtacatgc gtttacggac ctgggcatga acgtgcagtt
      661 actgactttt ctcctgggct ttgtaattcc accccttgcc ttctttttat accggttaaa
      721 agaaattccc agtgtgaaac aggaagagaa tatttcatcc cgcgaattct ggatgtttat
      781 tggggcattg gtctttttcc tttccgcgat cgtgatcatt ttccaaacct ccaccccggt
      841 actgaataaa gtattgggta ctacgattgc ccctccggat gatgcagaat tcgcttataa
      901 cagtatacag gtgtatgtgg cgatcattat agccctgttg acggctgttt cccaatacct
      961 gaaatataag gataccagcc gctcctattt ctggaaaaaa atgctgattc ccgcaatgat
     1021 cactttattc cttggcggtc tctttgtgat ttttggcgaa atcaattacg ataaaaaagg
     1081 tccggttttc cagggcgcac tctggctggc tgttatctgc agtatctaca cgattgtagc
     1141 caatgcagca tacatatggc tgggtataaa aggaagtttg aaactttccg gaggatctat
     1201 tgcccacgta ggttttggaa tggtattgct cggtatcctg atctcttctt ccaacaaaga
     1261 agttatttcc aacaatatcg ggggcatccc tgctcccctg gctgagggcg aaaaccccag
     1321 ggaaaacctg accctggtaa aagatatgac agccaacatg ggtcgttatt ccctgaccta
     1381 cctgggtgat tcggcgcatc ccaaaaagca actctggttt tataagatcc gggtaaaaag
     1441 tattgatggc aaagaggatt ttgtactgag tccaaacgct tttgtaaatt ataaaggcaa
     1501 tgaaggactg atggccaatc ccgattcacg ccattattgg gatcatgatg tatttacgta
     1561 tatcacctcc attcctaatc cggaagcgaa aaaagatacg gccagtttca aaccttttgt
     1621 acgtaaagcc ggtgatacca ttttttacag taaaggattt attaccttaa atacacccat
     1681 ccggcgcgac agcgttcccc aggaactatt tggcaaaggc ggttacctta cagaacttcc
     1741 aatggtagtt catgctaaaa ccggatctaa ttatacgttg accagccggt tggcgattgc
     1801 acaaaacggc atttttgccc tcccggatac ccttcatgca gaagggttga ttatccagtt
     1861 acaaagcgta aatccggata atacggttga agttggaatt aaagaatccg atgccttact
     1921 taaatacgtg acgctcaagg cttatcgttt cccctttatt cgcttattgt ggtatggtgt
     1981 ctatattact gccattggca ttctgatcag tatggtccgc cggatccagc tcaaccggaa
     2041 aaagaaggcc ggagaagaaa tttaagttta cagaaacagc gccgggcctg gttttagtgt
     2101 ctgttcggaa ataccgtttc tgttttttaa ctttataagc gcatgcgtgc ccgtttcagc
     2161 cataccttca cattcccgct tttgttagtt tttttgctgg caaaggtgac ttccgtttcg
     2221 gcgcaggtgg tcttacctga accagcggtg gattccacgg attgggcgtt ggaatacatg
     2281 aaagaacaac ggaagaaatg gggacctaat gataccatca tactcccggc catctggtac
     2341 caggcagaga ttgtgaatta taaggaaatg gaaatggcct gggtctccaa cctctcaccg
     2401 gaaaaattac agaagtatat tcaggaatgg acccggctgc gcaatgcggt ttatgttacc
     2461 tacccctatg cccggattgc cggtggcaca atcaatgaaa tcaatacaaa actgaccggg
     2521 atcacttcaa aaaaagaacg gaagacggtt attaaatcca gggaaaaaca actgaaatcc
     2581 gaattcagcg agccactgag caatctctcc gtatatcagg gcaaaatact catgaagctg
     2641 ataaaccggc aaaccggcaa taactgctat gagattatca aggaatacaa agggggattc
     2701 aatgcccgtt tttaccaaac cattgccttc tttgtaggtg gcaacctgaa acaggagtgg
     2761 gacctgaagg aaaacaaaat ggacagggat attgagaata ttgtccggga aattgatggc
     2821 agctggtacg gcaatcaata cagggcccgc taacccaaag ccgcgaaaaa tagcgactct
     2881 cccccgaaac aaataccttt gccgggttta aaaccaacca tagttatgaa agtggatatt
     2941 ctggccatcg gtgtacatcc ggatgatgtg gaactcgggt gttccggcac actgatcaat
     3001 gaaattaaga acggtaaaaa agttgccatc ctggatctga cccaggggga actcggtacc
     3061 cgtggtacca ttgaaacccg ttatgaagaa gcggccaatg cggccatgat tatgggcgta
     3121 cacgcacgcg aaaatcttaa aatgcgggat ggcttcttcc ggaatgatga agcgcatcaa
     3181 ctgcagctca tttcagccat ccgcaaatac cagccttcag tggtgattgg taatattctg
     3241 aaagaccgac accccgatca cggacgggcc ggcagcctga taaccgacag ttgttttctt
     3301 tccggtctcg caaagattga aacaacagat gcagcgggaa atgaccaggt aaaatggcgc
     3361 ccggcctatg tgcttcaata tatgcaggac tggtaccatg agccggattt cttagtagat
     3421 atcagcgaag tttttgctca acggatgaaa tcaatagaag cgtataccac ccagtttcac
     3481 aatggcaatg ccccgcagga aggtccgcaa acctatatct ctaaacccgg tttccttgaa
     3541 gcggtggtag cccgggcccg gatgtttgga aaacggattg gcgtggatta tgctgaaggc
     3601 tttctcagtg aaaaaaaact ggggctacgg aacctcgatg cgctcattca aaatgaaacg
     3661 taagtcacga attagcagga ctttaacagg aactgattct gctcatgtcg tcgatatttt
     3721 ttgacgaagt ctgggcttta tgggttacct ttgtacatcg aaacaagcat cataatgaat
     3781 tttccaaaat tttcaggtaa cgcacagact tttcacgcag aactcaagaa aagaatcaac
     3841 gagtatttcc acgaagccgg taaatcttac actggcaatt tccagctcta ttttaaagca
     3901 gttgtattag tactggccta tatagccgta tatacccacc tggtttttta tacgccggcc
     3961 acctggctgg cactcacaga atgtgtggtg cttggcggac tgaccgctgc tattggtttc
     4021 aatatcatgc acgatgggat gcacggtagt ttcagccgga aaaaatgggt caattatatg
     4081 gccggacttt ccctgaattt cctcggggcc aacaacttca tgtggaagac caagcacaac
     4141 atcatacacc atacatacac caacattgaa gggctggacg atgatatcga agcccgtccc
     4201 ctgttgcgtc tttgtgatac acagaaatat tataaaatac accgttttca gcactggtat
     4261 ttctgggcgg cttattcttt actctatatc tggtgggttt tcgtaaccga ctataaaaaa
     4321 tatttccgga aaaagatcgg cgaagtgccc ctgaaaccca tgagtgcctg ggatcatatt
     4381 tctttctggg gatttaaagt attaaacatt ttcattttcg tggtaattcc cattgcccaa
     4441 cttggtttta catcctggtt gatcggctac ctggtctttg gactgtttgc tggctttgtt
     4501 ctgagtattg tgttccagct ggcccatacg gtagaagata cccatttccc tcatgctgat
     4561 gaccataacc ggatggaaga tgagtgggcg attcaccagt taaaaaccac ggccaatttt
     4621 gccaccaata ataaggttat ctcctggctg gtaggcggcc tgaatttcca ggttgaacac
     4681 cacctgttcc ctaaaatctc acacgtgcac tatcctgcta tcagcaagat tatcaagaaa
     4741 gcctgtgatg actttggtgt aacttatatt gaatacccca aaatgcggct ggctcttgcc
     4801 tcgcatgtac atcacctgaa gaatctcagc agaaaataaa atactgaatc ccgcggaagc
     4861 gggatttttt ttgtcccgca aagatttata agacaacccg atagtagaaa actccaaaag
     4921 gatcagcaag gcgttagggt gtcaaagtct gtttccggcc gatagtacag tcccgaactt
     4981 aaagaatgtc ttcctggctt tgcgggaaat cctgacacag ctcaaaaacc cgggcagatg
     5041 attctttttg cagcaaccag aaaacccctg taacttgcac cgtttttcaa tatgaaatca
     5101 aaatggtaga cttatccact tacgacccta acagcgtcag taaccctaat aacaacattt
     5161 tcggacttcc cagtgatgag gagagtgccc ggctgattat tctgcccgta ccctgggaag
     5221 taaccgtaag ttatggtgcc ggtaccgccc gagctcctga agccattctc aaagccagtt
     5281 accaggtgga tctctttgat ccggaaatgc cggatgcctg gaaacaggga ttctatatgc
     5341 gtcctgtgga ccgaaagatc ctgatgaaaa gtgactacct gcgaaaagaa gccgaattat
     5401 acatcgatta tatttcaaag ggtgatgatg tgaacgccaa ccagttcatg tgcaaaaccc
     5461 tgaaagaagt aaatgaaggc ggggtttttc tgacggaatg gatgcatgcg caaagcaagg
     5521 ccctgctgga tcaggacaag ctggtgggta tactcggtgg cgaccacagc gtgccactcg
     5581 gctttatgaa agcattgggt gaaaaacacg gggagtttgg cattctgcag atcgatgcgc
     5641 actgtgatct tcgtgacaac tacgaaggct tcaaacattc ccatgccagt atcatgttta
     5701 atgcgctgaa agaagtcacc caactgcaac gggtagtaca ggttggactc cgggattaca
     5761 gccaggtgga atgggattat atcaagaaca gtgggtataa agtcatcgct tattttgacc
     5821 gcgaaatcaa ggaacgccag ttttgcggag atacctggaa acatattgcc gaagagatga
     5881 ttagtcaatt accggataaa gtatatatca gttttgatat cgacggactt gatcgtaaac
     5941 tctgtccaca caccggtacc cctgtacccg gcggatttga aatggaagaa gtgtattata
     6001 tgctgaaact gctccgccag agcggcaaga agatcatcgg cttcgacctt tgtgaagtgg
     6061 gcgtcagtga ttccgactgg aatgcaaatg tgggtgccag ggtgctgttc aaattgtgta
     6121 atctgctggt cacttccaac tcgtaatagt catgaaagtt gctgaagatt atgtattgtc
     6181 gctgcccaac acaaaagcca agttctatga tacgatgggc tggctgttga tcattattca
     6241 tgtaaccgca ttcagcacac ttgcctacta tgaaagtaat cccacacagc gctggtatgc
     6301 tttattgggt attctgttga ttgctatccc gcttattttt gaattggtta ttcgcaaatc
     6361 ccatcagaaa tcaaccgcca ttcaatctgc cctcacttcc ggcaccctcg tctggctgct
     6421 tttccttcat tattatacca ttggctttat cagccttgca ctggtattat tgttttatat
     6481 agcccggagg gatttgaagg tccttgttaa taaagaaagg attgaatacc cgagtttccc
     6541 acccaaaaaa atcaaatggg tcgatctgaa taatattatc ctgaaagacg gattattgac
     6601 catagacttt aagaataata agatcattca gcaactgatc gatgaaaata atctggtgga
     6661 tgaaaatgaa ttcaataatt attgtcgtga tcgattagcg aaaggctgat atgtcgcacc
     6721 tacggcgctc tgtacatttt tcctcgcccc tgggctaatg atatatcgca cctacggcgc
     6781 tttgtacatt gctctttgcc cgccactcgt aactcgaaac tcgtagctgg ttccacccga
     6841 ctattgacta ttgactaacg actatcgact atctttgccc catgcctatc tcccaatcac
     6901 cctacatcct tta
//
LOCUS       DAOPMW010000152         6867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12577_length_6867_cov_12.706841, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000152 DAOPMW010000000
VERSION     DAOPMW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12577_length_6867_cov_12.706841"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..986
                     /locus_tag="PLU11_08400"
     CDS             42..986
                     /locus_tag="PLU11_08400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPN59179.1"
                     /translation="MSKILCIGEALIDMICTDKGRSLKDGENFLKKPGGAPTNVAAAI
                     AALGGQVELAAKVGVDPFGQHLVEVMKDFGVDTRYMLRDADHFTTFAFVSLMENGERD
                     FYFNRGADGQLSKSDIEIINLEQTSIVHFGSATGFLPGPLQEAYLYLLQQAKARNIFV
                     SFDPNYRHLLFKDHIGSFIQQSWYFLEQCDFFKVSDEEALLLTGASGLEAAAQQFLEK
                     TNAVFAITLGKEGTLLGRDGKTSIISSIAVNPVDTTGAGDAFVGAVLYQLTGSAGKEL
                     STKEWETIILNANKAGARTCEYLGAMEAFRHLSNDIFK"
     gene            996..2942
                     /locus_tag="PLU11_08405"
     CDS             996..2942
                     /locus_tag="PLU11_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN59180.1"
                     /translation="MYNHQLHQQINTVLQNEQIDIGGKDNHFFTRFIANATAIDALFH
                     EIYGRTEKGMAQFDQLIHLITRAYLQRPDQLLARDAQKEAKGNWFLSNELAAMSLYVD
                     RFGGNLKGLAGKLDYFQELGVNFLHLMPVFESPEGESDGGYAVSDFRKVDQRFGTLSD
                     LRELQRRMQKEGMYLMLDIVLNHTSHHHEWANKAKAGDKKYQDYFYMYNNRQLPDQFD
                     RSMPEIFPESAPGNFTWNEEMQKWVMTVFHRYQWDLNYSNPQVFLEMLDHIFFYANLG
                     VDMLRIDAPAFIWKQPGTTCQNLPQAHTLLRLIKQCVEVSAPGMALLGEAIVAPKEIM
                     KYFGTGLYKARECDFAYNATHMALQWDALATGDTRVMLAAQHEILQKPYGSSWITYTR
                     CHDDIGLGYDDYMIQHAGFNPFEHRRFLKDYFSGARADSPARGALFSVNPKTNDARIS
                     GSLASLCGLEKALEEENVFNIEQSISKILMMQAHSFFLGGLPMVFYGDELGYTNDYSY
                     LQDPGKSYDNRWMHRPVIDWKKNERISEEGTIEYQVFQATKKLIQIRKKLFAIGDYKN
                     LTWMTPHNIHVAGYLRTFEEQKIYCLFNFSDQPSYLTWFAFKEHGAAPHTLFDHWTEK
                     ELAIGYDHQYLILEPYQFMILEAK"
     gene            complement(2949..4457)
                     /gene="treF"
                     /locus_tag="PLU11_08410"
     CDS             complement(2949..4457)
                     /gene="treF"
                     /locus_tag="PLU11_08410"
                     /inference="COORDINATES: protein motif:HMM:NF009773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HPN59181.1"
                     /translation="MTEKKVNPFCNPLFEAVQLNRIFPDGKTFVDCQPKQAYEEILEA
                     FHTENKKPGFDLKQFVLNHFDLPVPHSVYYSSDTTKPVATNIESLWDVLTREPDTENG
                     SLIPLPFPYVVPGGRFGEIYYWDSYFTMLGLKTSGRYDMIENMIGNFSYLIDTLGYIP
                     NGNRTYFIGRSQPPFYAQMIKLLADKKGESIYLQYLPQLEKEYQFWMKGAAALDKENK
                     ALFRCVLMPDGSVLNRYKDEWSSPRPESYREDVELAQHTTETVKPSVYGHLRAGAESG
                     WDFSSRWFKKSGDFSSIHCTDIIPVDLNCLLLNLEQTLAHAYALKGETGHSIRLVLAA
                     DKRKKSLEKYCWNESAGFYMDVDWVEGKQKTAMTLAGISALYFKLADNQKAERIQEQI
                     RGLFLKEGGVVTTLEATGQQWDAPNGWAPLQWMTIKGLENYGFTELASEIARRWIRLN
                     TAVFKRTGKLMEKYNVVNTHLEAGGGEYEGQDGFGWTNGVLLALISQYGQAE"
     gene            complement(4469..5740)
                     /locus_tag="PLU11_08415"
     CDS             complement(4469..5740)
                     /locus_tag="PLU11_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN59182.1"
                     /translation="MQNTKIKISLFLNYFVFAILLNSVGTVILQVQTNYGVTKGAASV
                     LEAYKDLSIAITSFLVASYVVRMGYKKTMLSALAFVTLICLAMPSMNSFWMTKMLFAV
                     TGLSFALIKVSVFATLGLVTTGKKEHLSLMSFLESFFMVGVLSGNFIFSAFVDNDHPE
                     SKAWFSVYYLLAGLSAIAFILLLSTPLDESNVQQAEKQESTSLTADFSGMLKLVILPL
                     VLVFIGCAFFYVLIEQSIMSWLPTFNKDVIKLNTSLSIQMASIMSASIALGRFLAGLL
                     LRKIEWIYVLTGCLLAAGALVLITVPLTNNISGQTISLLKDVPLAAYAFPLIGLFLAP
                     VYPAINSVILSNLPAHKHASMSGLIVVFSALGGTTGSIITGHIFDAWGGQTAFYSSLV
                     PICILMVLLYLFKKVQPVTGNTPISTSGSSH"
     gene            complement(5868..>6867)
                     /locus_tag="PLU11_08420"
     CDS             complement(5868..>6867)
                     /locus_tag="PLU11_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN59183.1"
                     /translation="LFMQQSTLKKLSEVLGISISTVSRALKNHPDISESTKTRVRELA
                     TALEYEPNHNAVQLRTRQSHVLGILVPSIDNFFYDSFIAAVEGMAREQGYSVLIMQSR
                     DQLDVENANLHLFRKNMVSGGFASVSIETEEMNAFQKLSDQKIPVVFFDRVPELPGFH
                     KVCLSDAKAARLAAETIILKKKKRVLGLFGHPHLSITKMRAASFRETLEKKAPVPQFC
                     IDYPEGIEKSKEVTLAALKSHTRPDVIFCMGDMILVGVMQAIHELQLKVPEEISVISI
                     SNGLFPTMYNPKITYVETNGGKLGKLAFSQMMSAIRHEPFPEISYLEADLIEGGSI"
BASE COUNT         1841 a         1611 c         1651 g         1764 t
ORIGIN      
        1 atagccctcg aggatatgga taaaacaacc gtattacatg tatgagtaag atactttgca
       61 tcggagaagc gctgattgat atgatctgca ccgataaagg ccgttccttg aaggatggag
      121 aaaatttttt gaaaaagccc ggcggtgctc ccaccaatgt ggcagcagct atcgcagccc
      181 tgggcggaca ggtagaactg gccgctaaag tgggcgtgga tccatttgga cagcacctcg
      241 tggaagtgat gaaggacttc ggcgtggata cccgttacat gctgcgggat gcggatcatt
      301 tcaccacgtt tgcttttgtc tccctgatgg aaaatggcga acgtgatttt tattttaacc
      361 gcggagccga cggtcaactc agtaaatcag atattgaaat aataaacctg gagcaaacca
      421 gcattgtgca ttttggttca gccaccggtt tcctgccggg cccactgcag gaagcctacc
      481 tgtatttatt gcaacaggca aaggcccgca atatatttgt gagctttgat cccaattacc
      541 ggcacctgtt gttcaaagac catatcggtt cttttattca acagagctgg tatttcctgg
      601 agcagtgtga tttctttaaa gtaagcgatg aggaagccct gctcttaacc ggcgcatccg
      661 ggctggaagc agcggcacag cagttcctgg agaaaacaaa tgcggttttt gctatcaccc
      721 tgggaaaaga agggaccctg ttgggacggg atggtaaaac cagtatcatt agtagtattg
      781 ccgtaaatcc cgttgatacc accggtgcag gcgacgcttt tgtaggcgcc gtactttacc
      841 agctcaccgg cagtgccgga aaggagctta gcacgaagga atgggaaacc attattttaa
      901 atgcgaataa agcgggggcc cggacctgtg aatacctcgg agcgatggaa gcattccgcc
      961 atttaagtaa cgatatattt aaataagcaa cgcacgtgta caaccaccag ttacatcagc
     1021 agattaatac ggtcctgcaa aacgaacaga tcgatatcgg gggaaaggac aaccatttct
     1081 ttacccgctt tatcgccaat gccacggcga tagatgcgct ctttcatgaa atatatggtc
     1141 gtactgaaaa agggatggca caattcgacc agctgatcca tctgattacc cgtgcttatc
     1201 tgcaacgacc ggatcaattg ctggcaagag atgcgcaaaa ggaagcaaag ggaaactggt
     1261 tcctgagcaa tgagctggct gccatgagtc tttatgtaga ccgctttggc gggaacctga
     1321 aaggactcgc cggcaaactg gattattttc aagaactcgg ggtcaatttc ctccacctga
     1381 tgccggtctt tgaaagtccg gaaggcgaaa gtgatggcgg ttatgccgta tccgatttcc
     1441 ggaaagtgga tcaacgcttt ggtaccttga gtgatctgcg ggagctgcaa cgccggatgc
     1501 agaaagaagg catgtacctg atgctggata ttgtgctcaa tcatacttcc catcaccatg
     1561 aatgggctaa caaagccaag gccggggata agaaatacca ggactatttt tatatgtata
     1621 ataaccggca gctgccggac cagttcgacc gttccatgcc ggagattttt cctgaatcag
     1681 cacccggcaa tttcacctgg aatgaagaaa tgcaaaaatg ggtgatgacg gtttttcatc
     1741 gttaccagtg ggacctgaat tattccaatc cgcaggtttt tcttgaaatg ctggatcata
     1801 ttttctttta tgccaacctg ggggtggata tgctccggat tgatgcgccg gcctttatct
     1861 ggaaacaacc cggcaccacc tgccagaacc tgccgcaagc acataccctt ctccggctga
     1921 tcaaacaatg tgtggaagta tctgcaccgg gaatggccct gttgggagaa gcgatcgtgg
     1981 ctccgaaaga aatcatgaaa tattttggta ccggtttgta taaagcgagg gaatgtgatt
     2041 ttgcctataa tgcaacgcat atggcccttc aatgggatgc cctggctact ggtgataccc
     2101 gggtgatgct ggcagcgcaa catgaaatcc tgcaaaagcc ttacggcagt tcctggatta
     2161 cctatacccg ttgtcatgat gatatcggac tgggttacga tgattatatg attcagcatg
     2221 ccggctttaa tccgtttgaa caccggcgct ttcttaaaga ttatttttcc ggtgcaagag
     2281 cagattcgcc ggcccggggg gcgttgtttt cagtaaatcc gaaaaccaat gatgcgcgga
     2341 tcagtggttc actggcttcc ctttgcgggc tcgaaaaagc gctggaggaa gagaatgttt
     2401 tcaatattga gcaatccatt tccaaaatcc tgatgatgca ggcccatagt ttttttctgg
     2461 gcggactgcc gatggttttt tacggggatg aactgggcta taccaatgat tattcgtacc
     2521 tgcaggatcc cggtaaaagt tacgataacc gctggatgca ccggccggtg attgactgga
     2581 agaaaaacga acggatcagt gaagaaggaa cgattgaata ccaggtgttc caggcaacta
     2641 aaaaactgat tcagatccgg aaaaaattat ttgccatcgg ggattacaaa aacctgacct
     2701 ggatgacgcc gcataatatt catgtggccg gttacctgcg gacttttgaa gaacagaaaa
     2761 tttattgcct gtttaatttc agtgaccagc cttcttatct tacctggttt gcttttaaag
     2821 aacatggggc cgcaccccat acattgtttg atcactggac ggaaaaggaa ttagcaatag
     2881 ggtatgatca ccagtacctg atacttgagc cttaccagtt tatgatactg gaagcaaagt
     2941 aatgcagcct attctgcttg cccatattgg ctgatcaggg cgagcaatac tccattggtc
     3001 cagccaaaac catcctggcc ttcgtattca cctccgccgg cttcgaggtg agtattgact
     3061 acattgtatt tctccatgag tttaccggtt cgtttgaata cagcggtatt caaacgaatc
     3121 caccggcggg ctatttcact ggccagttca gtaaaaccat aattttccaa ccctttaatg
     3181 gtcatccatt gcaacggggc ccagccattg ggggcatccc attgctggcc ggtggcttca
     3241 agtgtggtaa caacaccccc ttctttcaga aataaacccc ggatctgctc ctgaatcctt
     3301 tctgctttct ggttgtcggc cagtttaaaa taaagcgcac tgatacccgc cagggtcatt
     3361 gccgttttct gctttccctc tacccagtca acatccatat aaaacccggc actttcattc
     3421 cagcaatatt tttccaacga tttttttctt ttatccgcag cgagtaccag ccggatggaa
     3481 tgaccggtct ctcctttgag tgcatatgca tgcgctaatg tctgctccag gttgagcaat
     3541 aaacaattca ggtcaaccgg tatgatatcg gtacaatgta tactggagaa gtccccggat
     3601 tttttaaacc agcggctgct gaagtcccag ccagattcag ccccggcgcg gagatgtcca
     3661 tataccgatg gcttaactgt ttcagtggta tgttgcgcca gttccacatc ttcccggtaa
     3721 gattccggcc gcggactgct ccattcatct ttgtatcggt tcaaaacgga tccatcaggc
     3781 atcagcacac aacgaaacag ggctttgttt tctttatcca gtgctgctgc tcctttcatc
     3841 cagaactgat attctttttc cagttgcggc aggtattgca ggtaaattga ctctcctttt
     3901 ttatcagcca gcaatttgat catctgcgca taaaaaggtg gttgggagcg gccaataaaa
     3961 taagtccggt tgccattggg aatatagccc agggtatcga tcaggtacga aaaatttccg
     4021 atcatgtttt caatcatatc atatcgcccg gaagttttca gaccaagcat ggtgaaataa
     4081 ctatcccagt aatagatctc tccgaaacgt ccacccggca ccacgtaagg aaaggggagg
     4141 ggaatcagtg agccgttttc cgtgtccggt tcccgggtca gcacatccca taatgattca
     4201 atattggttg caaccggttt ggttgtatcg ctgctgtaat aaaccgaatg gggcaccggt
     4261 aagtcaaaat ggttcagcac gaattgcttc aggtcaaaac cgggtttctt gttttctgta
     4321 tgaaacgctt ccagaatctc ctcgtaagct tgtttgggct ggcaatccac aaaggtcttt
     4381 ccatccggaa aaatccggtt cagctgtacc gcttcaaata agggattaca gaaagggttc
     4441 acttttttct cagtcataga tcgatggatt aatggctgct tccactggtg gaaatggggg
     4501 tgttaccggt gaccggttga acctttttaa ataaataaag caataccatc aatatacaga
     4561 tgggcactaa ggaagaataa aaagcggtct gtcctcccca ggcatcaaag atatgaccgg
     4621 taataatgga acccgtcgtg ccacccaacg ccgaaaaaac aacaatcaga ccggacatcg
     4681 acgcatgttt atgcgccggc agattactta agatgaccga attgatagcc ggataaaccg
     4741 gggccaggaa gagtccgatc aatgggaatg cataggcggc aagtggtaca tctttcagca
     4801 gactgatggt ttgcccgctg atattattgg tcagcggtac cgtgatcagt accagggcac
     4861 ctgctgcgag caggcaaccg gtaagaacat agatccattc aatcttacga agcaagaggc
     4921 cggccaggaa acggccaagc gcaatcgaag cagacatgat actggccatc tgaatactga
     4981 gactggtatt gagtttaatc acatccttgt taaaggtcgg caaccagctc atgatacttt
     5041 gttcaattaa tacatagaag aaagcacatc cgataaatac caatacgagt ggaagtatga
     5101 ccagtttcag catcccgctg aaatcagctg ttaaagatgt tgattcctgt ttttccgcct
     5161 gttgaacgtt gctttcatcc agcggggtac tcagtaataa aataaatgca atggcagaaa
     5221 gaccggcaag caggtagtac accgaaaacc aggctttgga ttccggatgg tcattgtcca
     5281 caaaggcact gaaaataaaa ttgccactca gcacacccac cataaaaaag gattccagga
     5341 aactcatcag actcaggtgt tcttttttcc cggttgtcac taatcccaat gtggcaaaca
     5401 cacttacttt gatcagggca aagctcaatc ccgtaacggc gaaaagcatt ttggtcatcc
     5461 agaaactgtt catggacggc attgcaagac aaatgagggt tacgaaagcc agggcggaca
     5521 gcatggtttt tttatagccc atccgcacca catacgaagc aaccaggaaa gaagtgatgg
     5581 cgatactgag atctttatag gcttccagca cactggcggc ccctttggtt actccataat
     5641 tagtctgtac ctggagaatg acggtaccta cactgttgag taatatggca aagacaaaat
     5701 aattgaggaa aagggagatt ttgatcttgg tattttgcat tgcaatcgtt taaggtgaac
     5761 taccggctga agacagtaaa gtttcaatgg ttctaaaata aaccatcaat cccgggcagg
     5821 caaataccgg ccggacctat aacgtttgcg gggataacta ttcggtgtca gatggagccg
     5881 ccttcgatca ggtcggcctc gagataggag atttccggga acggctcatg tcggatggcc
     5941 gacatcatct gcgaaaaagc cagtttgccc agtttgccac cattggtttc cacataggtg
     6001 atcttgggat tgtacatcgt ggggaagagg ccgttgctga tactgatcac ggaaatttct
     6061 tccggcactt tcagttgcaa ttcatgaatg gcctgcatca cgcccacgag gatcatatcg
     6121 cccatacaga agatcacatc cggacgcgta tggctcttca atgccgccag ggtcacttcc
     6181 tttgatttct caatgccttc cgggtagtcg atacagaact gcggcacagg tgcttttttc
     6241 tcgagcgtct cccggaatga agccgcccgc attttggtga tactcaggtg cggatgacca
     6301 aacaatccca gtacccgttt ctttttcttc aggataatcg tctccgccgc caatctcgcg
     6361 gctttggcat cggagagaca aactttatga aagccgggca actccggtac ccggtcaaaa
     6421 aacaccaccg ggattttctg atcactcagt ttttggaatg cattcatctc ttctgtttca
     6481 atggatactg aggcgaaacc cccggagacc atattcttcc ggaacagatg aaggtttgca
     6541 ttttccacgt ccagctgatc acgcgactgc atgatcaaaa cagaatagcc ctgttcacgg
     6601 gccatccctt caacggctgc tataaatgaa tcgtaaaaaa aattatctat agagggaacc
     6661 agtatgccca gtacatgact ctgccgggtt cttagctgga ctgcattgtg attgggttca
     6721 tactccaggg cagtggccag ttcccgaacg cgggtcttgg tgctttctga aatatcagga
     6781 tgatttttca gggccctgga tacagtagag atactgatac ccagcacttc tgatagtttt
     6841 ttcagcgtgc tttgctgcat gaataat
//
LOCUS       DAOPMW010000153         6825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12684_length_6825_cov_8.297194, whole genome shotgun sequence.
ACCESSION   DAOPMW010000153 DAOPMW010000000
VERSION     DAOPMW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_6825_cov_8.297194"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLU11_08425"
     CDS             complement(<1..645)
                     /locus_tag="PLU11_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59184.1"
                     /translation="MKRNCLVSLLLLVFFFSSCKEEETEFSVNSCSVNPPFVESLGYE
                     PQFAYFSTSDIRTMGLVLNQSKQKGNPASPVEKSYQHPSWRKAGWLAPIQLDNKGNLF
                     TAPAPFINVLNNPIAGQNTIWKVNGSTGVMDPFIALPMPDTSLQHPFGIIGLAFLCET
                     NTLYASTLAGSDRLYERGGIYAIDAVTGKITDQLMATDAMGMGISYITGKRCLYY"
     gene            complement(649..2454)
                     /locus_tag="PLU11_08430"
     CDS             complement(649..2454)
                     /locus_tag="PLU11_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59185.1"
                     /translation="MLIRSGKTGLHRLILLPVVLYMALLLLYACGNAGPASKEWPAYN
                     DDAKNAVLLPIDSFSKQPFIQERVKEITLALAQPIPVLLCRDSMNSQQQLAEMIALRN
                     TDFTRHVLDPQTRQPFRNEIFGVYPARQSDLVTVKEPYILSSVYRVEMYNYALNLTSV
                     AMVDITQQRVLAGYHQEEVQPDISGRLRDLAVKIAVESPEVQEALGFKPGETAALMAS
                     TKTALNRSRCERSRHLCVAPTFVKGDKALWAIVDLTDHRLVGIRWTTVGEPGAAVPLV
                     TERKLQDDKITSCYCEVEQPLKKDGWEMKYMLTGSDGLRISEVKYNGKPVLRSAKLVD
                     WHVSYSGTDGFGYSDAVGCPYFSQAAVVAWEVPQVAALKDEQNKVVGFVLEQTFRSEG
                     WPTPCNYNYRQRYEFYLDGRFRVSCASIGRGCGNTGTYRPVFRIAFEGARNSFYEYKE
                     NSWLQWQKEQWQLQGSLTQYTPEGYQYRIGEGAAGYYMEPGKGQFADGGRGDFAYVYV
                     TKHTAGRDEGENDLVTIGPCCNTDYHQGPDKFIEPAPEDISQGSLVLWYVAQMRNDDR
                     KGNEYCWAENVLENGVWQTRTYPCFAGPMFIPYKQ"
     gene            complement(2435..3604)
                     /locus_tag="PLU11_08435"
     CDS             complement(2435..3604)
                     /locus_tag="PLU11_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HPN59186.1"
                     /translation="MKEVKSLFAVKLIRTVSGTIVLLFACCIAGLNSCQTKQSRVKDF
                     LSWLPEQHRDSVYSDPANPYSEEKRVLGRYLFYDNRLSANNTKACASCHAQEYSFTDN
                     YTRSAGVYGDLHQRNSRPLINLVYNRYLTGADSTLHFPEAQMLLPLLNEHPPEMGVKG
                     KEAHILSRLREDTRYPGLFAAAFPDAADPVSWEHIRQSIAVFVKTIISADAPWDRYLR
                     DSSQYPLSAAAKRGRQLFYSPALQCYSCHAGINFSTPVLKEASGKTDFYFNTGLYNIG
                     GKGGYPAADRGLYQLTGNVADEGKFRVPTLRNLAFTAPYYHDGSALSLADVIRVYERG
                     GRLQPGGDGRSNPYKHELIKGFTLTAAEREDLISFLYSLSDTALIKNEQYANPFR"
     gene            complement(3673..5250)
                     /gene="atpA"
                     /locus_tag="PLU11_08440"
     CDS             complement(3673..5250)
                     /gene="atpA"
                     /locus_tag="PLU11_08440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPN59187.1"
                     /translation="MPEIKPDEISAILRQQLSNFNANADLEEVGTVLQIGDGIARVYG
                     LGNVRYGELVEFETGVRAIALNLEEDNVGVVLMGETAGIQEGSKVKRTGQIASIKVGE
                     GMVGRVVNTLGQPIDGKGPITGERYEMPLERKAPGVIFREPVKEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAICIDTIINQKEFYKAGKPVYCIYVAIGQKASTIAGVMKT
                     LQDNGAMDYTVIVAASASDPAPLQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVINDDAVAKNMNDVPDSIRHLIKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETNLFLSGIRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQALYRELEAFSKFGGDLDAATKNVIDKGARNVEVLKQPQY
                     SPFSVEKQVAIIYLGTNGLLKEVAVKRVKEFEEHFLMEMENKLPDVLAEFKKGNLPEE
                     GLKKMVELANGLIPQYK"
     gene            complement(5278..5841)
                     /gene="atpH"
                     /locus_tag="PLU11_08445"
     CDS             complement(5278..5841)
                     /gene="atpH"
                     /locus_tag="PLU11_08445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPN59188.1"
                     /translation="MQNPRLASRYAKSILDLAIERGELEKVYADMQWLQAVCKSNRDF
                     VNLLRSPIIKNDTKRKIIEAVTAGNISELTAGFNRLLVAKNRERFLPEIVNAFIAAYK
                     AHHKIRTVQLTTATPVTDSMRSAIVEQVKKSAGFEKVEVEEKVNPELIGGFVLQVGDQ
                     LIDASVAYDLRAIAKQFENNDFIYKIR"
     gene            complement(5955..6449)
                     /gene="atpF"
                     /locus_tag="PLU11_08450"
     CDS             complement(5955..6449)
                     /gene="atpF"
                     /locus_tag="PLU11_08450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPN59189.1"
                     /translation="MELLLPKLGLLAWTLLAFLIVFFLLSKMAWPAIIKGLRDRENSI
                     ADSLATAQKVKEEMALMKSENEALLAKAREERAQLLKEARETKDRIINEAKEQAKVEA
                     GKVVAEAQAAINAQKMAALTEVKNQVGKLVIEVSEKVLRKELGNKEAQEAHIKGLVDG
                     VKLN"
     gene            complement(6586..6798)
                     /gene="atpE"
                     /locus_tag="PLU11_08455"
     CDS             complement(6586..6798)
                     /gene="atpE"
                     /locus_tag="PLU11_08455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPN59190.1"
                     /translation="MANAGGAIGAGLAAIGAGIGIGQIGKGAVEAIARQPEASNDIRG
                     NMILTAALVEGAALFAIIVGFLAMVL"
BASE COUNT         1666 a         1820 c         1516 g         1823 t
ORIGIN      
        1 ataatacaga caacgcttgc cggtgatata ggaaatgccc atccccattg catctgtggc
       61 cataagctga tcggtaattt ttccggtcac tgcgtcgatc gcgtatatac ctccccgttc
      121 atataaccgg tcactgccag ccagggtaga tgcatataaa gtattagtct cacacaaaaa
      181 ggccagcccg atgatcccaa acgggtgctg aagactggta tcaggcatcg gcaatgcaat
      241 aaacggatcc attacccctg ttgaaccatt tactttccag atcgtgtttt gtccggctat
      301 cggattattg agcacattaa taaatggagc tggtgctgta aagagattgc ctttattgtc
      361 cagttgtatc ggagccagcc agcctgcttt tcgccaggat ggatgctgat aagatttttc
      421 aaccggtgaa gccgggttgc ccttttgctt tgattgattt agcaccagtc ccatcgtccg
      481 gatatcggat gtactgaaat aggcaaattg cggctcatag ccgaggcttt ccacaaaggg
      541 cggattcacg gaacagctat tgactgaaaa ttccgtctct tcctctttac aggatgaaaa
      601 aaagaagacc aacaacagca gggatactaa acagttcctt ttcatgtatt attgtttata
      661 cggaatgaac atagggccgg caaaacaagg ataggtcctc gtttgccaca cgccattctc
      721 caatacattt tccgcccagc aatattcatt tcccttccgg tcatcattgc gcatttgtgc
      781 cacataccaa agcaccagtg atccctgact gatatcttcc ggtgccggtt caataaactt
      841 atccggaccc tggtgataat ccgtattaca acagggacca atcgtaacca ggtcgttctc
      901 gccttcatcg cggccagccg tatgctttgt aacatataca taggcaaaat cacccctgcc
      961 tccatcagca aactgcccct tgcccggttc catataataa ccagccgctc cttctccgat
     1021 tctgtactga taaccttccg gagtatactg cgtcagacta ccctgcaact gccattgttc
     1081 tttctgccat tgcagccagc tattctcctt atactcataa aagctattgc gtgctccttc
     1141 aaaagcgatc cggaataccg gccggtaggt acctgtattc ccacagcccc tgccgatact
     1201 ggcacaggac acacggaacc ggccatccag gtaaaattca tagcgttggc ggtaattata
     1261 attacagggt gtaggccaac cctcactgcg gaatgtttgc tccagcacaa agcccactac
     1321 tttattctgc tcatctttca aggccgccac ctgcggcact tcccaggcta ccacagcggc
     1381 ctggctgaaa taaggacaac ctaccgcatc actgtaacca aacccatccg taccactata
     1441 actcacatgc cagtctacga gtttcgcact gcgtaaaacc ggtttcccgt tatattttac
     1501 ttcactgata cgcaatccgt cactcccggt cagcatatac ttcatttccc agccatcttt
     1561 ctttaatggc tgttctactt cacaataaca ggaagtgatt ttatcatcct ggagtttgcg
     1621 ttcagttacc aacggtacag cggcaccggg ctctccaacc gtggtccagc ggataccaac
     1681 caggcggtga tcagtgaggt ccactatggc ccaaagcgct ttatcaccct tcacaaaagt
     1741 gggtgccaca caaagatgcc ggctgcgttc acaacggcta cggttaagcg cagttttggt
     1801 actggccatc agggctgcgg tttcaccggg tttaaagcca agcgcttcct gtacctccgg
     1861 tgactccacc gcaattttta ccgccaggtc gcgtagacgt cctgaaatgt ccggttgtac
     1921 ttcttcctgg tgataaccgg ccagtacccg ttgttgtgtt atatcgacca tagccacaga
     1981 agtcaggttc agggcataat tgtacatttc aacccggtac acggatgata agatataagg
     2041 ttcctttaca gtaaccaggt cgctttgccg cgccggatac actccgaaaa tttcattgcg
     2101 gaaaggctga cgggtctgcg gatccaaaac atgtctggtg aaatccgtat tgcggagcgc
     2161 aatcatttca gccaattgtt gctgactgtt catactatcc cggcaaagca gcaccggaat
     2221 cggctgagcc aatgccaggg taatctcctt cacccgttcc tggataaagg gctgtttgct
     2281 gaaactgtca atgggtaaca aaaccgcatt ctttgcatca tcattataag caggccattc
     2341 tttggaagcc ggtccggcat ttccgcaggc atacaataac agcagggcca tatacagtac
     2401 aacaggcaac agtatcaacc gatgcaaacc ggtcttaccg gaacggatta gcatattgtt
     2461 cattttttat caaagcagtg tcggacagac tgtaaagaaa tgaaatcagg tcttcccgtt
     2521 cagctgcggt taatgtaaat ccttttatca gttcatgctt ataggggttt gaccggccat
     2581 ctcccccggg ttgtaaccgc cctccccgct catacaccct gatcacatct gcgagtgaca
     2641 aagcactgcc atcgtgataa taaggcgcgg taaaagccag gttacgcaat gtcggcaccc
     2701 tgaactttcc ttcatcggcc acattaccgg ttaattggta caatccccgg tcagctgcag
     2761 gataaccacc cttgccgcca atattataca atcccgtatt aaaataaaaa tccgttttgc
     2821 cagacgcctc ttttagtact ggcgttgaga aattaatacc tgcatgacag gaataacatt
     2881 gcaaggcagg tgaataaaac aactgcctgc ctcttttcgc tgcggcacta agcgggtact
     2941 gactgctgtc acgcaggtac ctgtcccatg gcgcatctgc acttataatt gtttttacaa
     3001 atacggctat tgactgccgg atatgctccc agctgaccgg atcagctgcg tcaggaaaag
     3061 cagccgcgaa caaaccaggg taccgggtat cttcccgcaa acgggaaagt atatgcgctt
     3121 cttttccttt tacacccatt tccggcgggt gttcattcaa taagggtaac aacatctggg
     3181 cttcgggaaa atgaagcgta ctgtcagccc ccgtcaggta ccggttatac acaaggttga
     3241 tcaggggacg actgttgcgc tgatgcaggt caccatacac tccggcagag cgggtatagt
     3301 tatcagtaaa agaatactcc tgtgcatggc aactggcaca ggcttttgta ttattagccg
     3361 ataaacgatt atcataaaac aggtaacggc ccagtaccct tttttcttca ctgtatggat
     3421 ttgcggggtc gctataaaca ctatccctgt gttgttccgg taaccaggac aagaagtctt
     3481 taacccgcga ctgttttgtc tggcaactgt tcagcccggc aatacaacat gcaaacagca
     3541 gaacaatcgt acctgaaaca gtacgtatca gttttacagc aaaaagtgat ttcacttctt
     3601 tcatccggtt caatcattaa aaaaagggct ctacccgcag gtacagccct tttgcaacat
     3661 catactgatg gattatttat actgaggaat cagtccgttg gccagttcga ccatcttctt
     3721 gagtccttct tccggcaggt tacctttctt gaactcagcc agcacatcgg gcagtttgtt
     3781 ctccatttcc atcaggaaat gctcttcaaa ctctttcacg cgcttcaccg ctacttcctt
     3841 caacagtccg ttggtaccca ggtaaatgat cgctacctgt ttttccacgg agaacggaga
     3901 gtattggggc tgtttcagca cttccacgtt acgggcacct ttatcgatta cattcttggt
     3961 agccgcatcg aggtcaccac cgaatttaga gaaggcttcc agctcacggt aaagggcctg
     4021 gtccagtttc agcgtaccgg ccactttctt catggatttg atctgagcgt taccacccac
     4081 acggctaacc gaaataccta cgttgatcgc cggacggata ccggagagga acaggttcgt
     4141 ctcgaggaag atctgtccat cggtaataga gatcacgttc gtggggatat aggcagatac
     4201 gtcacccgcc tgtgtttcga tgatcggaag agcggtcagt gaaccacccc ctttgatcag
     4261 gtgacggata ctgtcgggaa cgtcgttcat attcttagct accgcatcgt cattgatcac
     4321 tttcgcagca cgctcgagca gacggctatg cagatagaat acgtcaccag gataagcttc
     4381 acgacctgga gggcgacgga gcagcaggga tacctcgcgg taagccacgg cctgtttgga
     4441 caagtcatca tagatgatca gggccggacg accggtatca cggaagaact caccaatcgc
     4501 cgcaccggca aagggagcat agaactgtaa cggagcggga tcagaagcag aagcggcaac
     4561 gatcacggta taatccatgg cgccattgtc ctgcagggtt ttcatcacac cggcaatggt
     4621 ggatgctttc tgaccgatcg ctacgtagat acaatataca ggcttacctg ctttgtagaa
     4681 ttctttctga ttgatgatgg tatcgataca gatagctgtt ttaccggtct gacggtcacc
     4741 gatcaccagc tcacgctgac cacggccgat ggggatcatc gcgtcgatcg ccttgatacc
     4801 ggtttgcagg ggctctttta ccggctcacg gaagataaca ccgggggctt tacgctccag
     4861 cggcatttca taacgctcac cagtgatggg gcctttacca tcgatcggct gaccgagggt
     4921 atttaccaca cggcctacca tgccttcacc tactttaata gaagcgattt gtccggtacg
     4981 ttttacttta gaaccttcct ggataccggc ggtttcaccc atcagtacca cacccacatt
     5041 gtcttcttcg aggttcaggg cgatggcacg cacccctgtt tcaaactcca ccagttcacc
     5101 ataacgcaca ttacccagac catacacacg ggcaataccg tcaccaattt gcaatacagt
     5161 accaacttct tccaggtcgg cgttggcgtt aaagttgctc agctgctggc gcaggatggc
     5221 acttatttca tctggtttaa tttcaggcat atctgtctct ttttattact ttaaaaatta
     5281 gcggatctta taaataaaat cgttgttctc aaattgcttg gcaatggcac gcaggtcata
     5341 ggccacactg gcatctatca gctgatcacc tacctgcagc acaaaaccgc cgatcagttc
     5401 cggattcact ttctcttcca cttctacttt ctcaaaaccg gcagactttt tcacttgctc
     5461 cacgatagca ctacgcatac tgtcggttac aggcgtagct gtagtcagtt gtacggtacg
     5521 gatcttatga tgtgctttat atgccgcaat aaaggcattc acaatctcgg gcaggaaacg
     5581 ctcccggttc ttggccacca gcaggcggtt gaacccagcc gtcagttcgc tgatattacc
     5641 ggctgttaca gcttcaatga tctttctttt tgtatcattc ttaatgatcg gactgcggag
     5701 caggttcaca aaatcacggt tgctcttgca gaccgcctgc agccattgca tatccgcata
     5761 gactttctcc agctcacctc tttcgatggc cagatccagg atcgatttcg cgtatcttga
     5821 tgctaaacga ggattctgca tattgttcgg ttgagagttg agagttttga gttgagtgtt
     5881 tagagtcttg tgatcagggt tgaatggtca tcctaaagga ctcgtagctc gtacctcgta
     5941 actcgtacct tttcttaatt cagttttacg ccatccacca gtcccttgat atgggcttcc
     6001 tgggcttctt tattaccgag ttcttttctc agtactttct cacttacttc gatcaccagt
     6061 ttaccgacct ggtttttcac ttcggtaagg gcggccattt tctgggcgtt gatcgcagct
     6121 tgtgcttctg ccactacttt tcctgcttcc actttggcct gttccttggc ttcgttgatg
     6181 atacgatcct tggtctcacg ggcttctttc agcaactggg ctctttcctc acgggctttg
     6241 gccagcaggg cttcgttctc acttttcatg agggccattt cctctttcac tttctgggca
     6301 gtggcgagtg aatcagcaat gctgttctca cggtcacgca gtcctttgat gatcgcgggc
     6361 caggccattt tgctcagcag gaagaaaacg atcaggaagg ccaggagggt ccaggctaac
     6421 aaacctaatt tgggaagtaa taactccata aaacgtctga tttcaaatga atggcccctt
     6481 ccgggacttc ctcatttctt attggttatt tattatttgt catttaaata ctgcatcctc
     6541 cgccctgtgc tttggatgca gtaatttttt ttcgcggcaa cgatattata ataccattgc
     6601 gaggaagccc acgatgatcg cgaacagggc agctccctca acgagggctg cagtcaggat
     6661 catgttaccg cggatgtcgt tagacgcttc cggctgacgg gcaatggctt caacggcacc
     6721 tttaccaatc tgaccgatac cgataccggc accgatagct gctaaacctg caccgatggc
     6781 tccacctgcg ttagccattt ctgctaaaag aaataaatcc atgtt
//
LOCUS       DAOPMW010000154         6818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12704_length_6818_cov_8.615112, whole genome shotgun sequence.
ACCESSION   DAOPMW010000154 DAOPMW010000000
VERSION     DAOPMW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12704_length_6818_cov_8.615112"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLU11_08460"
     CDS             <1..256
                     /locus_tag="PLU11_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59191.1"
                     /translation="APSELYKPSSRTFDGNKMVFYFLQHRLSTLYYHKEKKEFRRIFN
                     HLAYNHTVRLLGSGKLLIYRVLLFLPAGFLHQIKKVISRK"
     gene            321..1457
                     /locus_tag="PLU11_08465"
     CDS             321..1457
                     /locus_tag="PLU11_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN59192.1"
                     /translation="MKVLFIQKMNGISGSELYMLQILPELKRRGYEVEMLIIFPSYGD
                     KNKRYIGYLAEHGIPTHEIYNHGALSPFLFYKFYKLLKKNKYDLVQSNLVHADFLAAM
                     TKFFLFRRMKMLSVKHGYHPAYQVKYGFDFTHLKSDPYYWVERFASNVANFNITISNG
                     LYKVFTDGGIVKKSKIRNIYYGLTLTEPVEQKTVEKMPEGPFALIVGRLVGFKCHNLL
                     IEAWKKVHAEIPELKLCIAGNGDLYEVLDKQIKEAGLEQTVLLLGHVPNPHPLMQASV
                     FTLVTSAWEGFGLILLESWLHKKAIVAFDAPAMNEVIDHGKNGLLARAGNTAELAKQI
                     IYLYQQPGLARQFGEEGFQKLHSYYTLARMTAETEEVYQQIVNS"
     gene            1469..2869
                     /locus_tag="PLU11_08470"
     CDS             1469..2869
                     /locus_tag="PLU11_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59193.1"
                     /translation="MEYGEYSTFNMIRTQDFYLVPLCLIVLMFFIRSMYMRYRDTPIQ
                     RYILPAFTLRIVGAIVYTMVIHYYYGFGDSHNYYQGVLDLHHAVSDDASFLKDIYGKL
                     KLERTDRIFPYFYYDGYGVSQYYMQEVRTYNVSRFALPFSLLFNKSFLCISFCISFLS
                     FHGSWRIFKMFYEMYPTIHKKIALATLFLPSLLFWGTSLLKDPICVAALGYFTYACYS
                     ALIKKKNITSSIIIIILSGLVLNNLKPYILLCMSAVFMLWVFLRVRDKIEDKTMRIVS
                     TSFFVAAAMAVGFFLSNSLADSEASQFSSDKLLGTVQKQQAIFGNASDEEGGGGGSKF
                     SVAKAESPLQLAASFPIGIVNTFFRPFPWDVRSPVAILSALEAFAFLALTWMCFRRLG
                     IKQTFNIMFSEPVIAFCFIFAILFGGIIGLTTTNFGALSRYKIPCIPFYAMAFILIMH
                     RSGKFNQPFIFSKRLF"
     gene            2961..>3226
                     /locus_tag="PLU11_08475"
     CDS             2961..>3226
                     /locus_tag="PLU11_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPN59194.1"
                     /translation="MCGIAGSVNFNLDIPRLTKDLWHRGPDEQASWAENNVQLHHHRL
                     AILDIASGKQPMQYREFTIIFNGEIYNHLDVRKKMGLQCVTHSDT"
     assembly_gap    3227..3326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3327..4634
                     /locus_tag="PLU11_08480"
     CDS             <3327..4634
                     /locus_tag="PLU11_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HPN59195.1"
                     /translation="MPYQQVKKLTAGSYAVISTRDLSVNVTRWWDIHPFYQQHSKDSF
                     EQALEKTDTLLKAAVKRRIESSDLEVGSFLSGGIDSGLVTAMASEYNNRLKTFTVSFA
                     GGEYDEAPLAKLVADRYGTQHNEIVISFDGLLDDVETILRNYGLPFFDSSAIPSYYVS
                     REARKHLKVILNGDGGDEIFGGYRRYVPFASYDFFKSGGLVKNLSSLVFPLMPISHDK
                     KNKYNFIYRLVDLARKEGLDTYFSSTLDIFEDHESGLKGNRDYSLRSIQQSFDQISQS
                     KLSGMQKMLNLDFDHILEASLLVKMDIATMAHSLEGRSPMLAKEILEYIPTLPDAYKV
                     KGTQTKYLLRTLAGKYLPPELIHQPKRGFEIPLKKWIDGELKDLIASYILAPNAYCRS
                     FLQPGFIENLWNKKLQTGAEKRAKMIWSLFALEVWYRKCYLKK"
     gene            5073..6230
                     /locus_tag="PLU11_08485"
     CDS             5073..6230
                     /locus_tag="PLU11_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN59196.1"
                     /translation="MPKLLRITTAPISLNVLLPGQMKYMREQGFDVLMVSSDGPELAT
                     VLEREGCRHHIIPMTRKMSPFADLRCLWLLYRLFLKEKPDIVHSHTPKAGLLAMLAAK
                     MAGVKFRLHTIAGLRYVTTKGLSRRILIAMEKLTGMAATHVWPNSGSMKAFIEANHLV
                     ATRKLEVIGWGSSNGVNLQRYHPSVLKEDKLNEVKALVNYDPDLFYFLSVGRLVHDKG
                     MDELVGAFVRVHAVHPHTRLILVGAFEDEVDPVSDKARELIKTHPGIIAAGWSDAVEY
                     FMQFSNVLVHPSHREGFPNVLMQAGAMRCPIICSAIDGNTDIVRHRETGLLFDVKNEE
                     ALFNQMQSAINEPDTLKAMAGQLLLQIQTHYDQRVVQSFLHEKYLQLLAAG"
     gene            6361..>6818
                     /locus_tag="PLU11_08490"
     CDS             6361..>6818
                     /locus_tag="PLU11_08490"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59197.1"
                     /translation="MKTTLKKPVAIVGYSGHAFVIIDILLSAGRLVTAYCDSEEKEFN
                     PYHLKYLGREGDVMHKLKDYDFFACVGHNGIREKIHTNLSQYLGNPINAIHPSAVISA
                     SVKLGEGVMIAANATLNPLVEIGRGVICNTSTSIDHECIIGDFTHIAPGAV"
BASE COUNT         1766 a         1602 c         1537 g         1813 t
ORIGIN      
        1 agcacccagt gaattgtata agccttccag ccggaccttt gatgggaata aaatggtttt
       61 ttatttcctg caacaccggc tcagcacttt atattatcat aaggagaaaa aagaattcag
      121 aaggatattc aaccacctgg cgtataatca tacagtccga ttgctcggtt caggcaaatt
      181 actcatatac cgggtgcttc tttttttacc agctggattt ctgcatcaga ttaaaaaagt
      241 gatcagtaga aaataaaatg acgttttaga aaacatccgc cgtttgtaaa atggtaagat
      301 gtttaagata tataaatact atgaaagtct tatttatcca gaaaatgaac gggatctccg
      361 gttccgaact ttatatgctg caaatcctgc cggagttgaa acgcagggga tatgaggtgg
      421 aaatgctgat catctttccc agctatggcg ataaaaataa acggtatatc ggctacctcg
      481 cagaacacgg cattccgacc catgaaatat ataatcatgg ggccctttct cctttccttt
      541 tttataagtt ttataaactc ctgaaaaaaa ataagtacga cctggtacaa tccaacctgg
      601 tacatgccga tttcctggca gcaatgacga aatttttcct gttccgccgg atgaaaatgc
      661 tcagtgtgaa acatggatac cacccggcct accaggtgaa atatggattt gatttcacgc
      721 acctgaaaag cgatccttat tactgggtgg agcgttttgc cagcaatgtg gccaatttca
      781 atatcactat ttccaatggc ctgtataaag tgtttaccga tgggggcatt gtgaaaaaat
      841 caaagatccg caacatttat tacgggctga ctctcaccga gcccgtagaa caaaaaaccg
      901 tggaaaaaat gccggaaggt ccctttgccc tgattgtggg ccgcctggta ggattcaagt
      961 gtcataattt gctgattgaa gcctggaaaa aagtacatgc ggaaattccg gaacttaaat
     1021 tatgtattgc cggcaatggc gacttatacg aagtgctgga taagcagatc aaagaagccg
     1081 gactggagca gaccgtgctg ctgctggggc atgtacccaa tcctcacccg ctgatgcagg
     1141 cgtcggtatt tacgctggtc acttccgcct gggaaggttt tggcctgatc ctgctcgaaa
     1201 gctggctcca taaaaaagcc atcgtggcct ttgatgcgcc ggccatgaat gaagtgattg
     1261 accatggcaa aaacgggttg ctggcacgcg ccggtaatac cgctgagctg gcaaagcaaa
     1321 tcatctatct ttaccaacag cccgggctgg ccagacagtt cggcgaagaa ggttttcaga
     1381 aactgcacag ttattatacc ctggccagga tgaccgctga aaccgaagaa gtgtaccaac
     1441 agattgtaaa cagttaaagc aaggacggat ggaatacggg gaatattcaa cctttaatat
     1501 gatcaggacc caggattttt acctggtacc cctctgcctg atcgtattga tgttctttat
     1561 ccgctcgatg tatatgcgat accgggatac ccccatacag cgctatatcc tgccggcctt
     1621 taccctgcgg atagtagggg ccatcgtcta tacgatggta atccactatt attacggatt
     1681 tggagacagc cataactatt accagggggt gctcgacctg catcatgcag tatcggatga
     1741 cgcctctttt ttaaaagata tttacggcaa acttaagctc gagcgtaccg accggatctt
     1801 tccctatttt tattatgatg gctatggggt aagccaatat tatatgcagg aggtaagaac
     1861 ttataatgta tcccgcttcg ccctgccttt ttccctgctc tttaataaga gttttctctg
     1921 tatttctttt tgtatcagtt ttctttcctt tcatggttcc tggcgcatct ttaaaatgtt
     1981 ctatgaaatg tatcccacga tacataagaa aatcgccctg gccaccctct tcctgccctc
     2041 gctcttattc tggggtactt ccctgctgaa agacccgatc tgtgtggcgg ccctggggta
     2101 ttttacctat gcctgttact ccgccctgat caagaaaaaa aatattacca gctccatcat
     2161 catcatcatt ttatccggac tggtactgaa taacctgaag ccctatatcc tgctctgtat
     2221 gtccgcggta tttatgctat gggtgttttt gcgggtcagg gataagattg aagataaaac
     2281 gatgcggatt gtctccactt ccttctttgt agccgccgcg atggctgttg gattttttct
     2341 ttccaatagc ctggcagatt cagaagcctc ccagttctct tccgataagt tattgggaac
     2401 ggttcaaaaa cagcaggcta tttttgggaa tgccagtgat gaagaaggcg ggggtggagg
     2461 ttccaaattt tcggtggcta aggccgaaag tccgttgcaa ctggcggcct cttttccgat
     2521 tggtattgtc aacacttttt tccggccctt tccctgggat gtgcgttccc cggttgccat
     2581 tctatctgcc cttgaagcct ttgccttcct ggcccttacc tggatgtgtt tccggcgact
     2641 gggcatcaaa caaaccttca atatcatgtt ctcggaaccg gtgatcgcct tctgtttcat
     2701 ttttgccatt ctattcggag gaattatcgg attaaccacc accaatttcg gggccttgtc
     2761 ccgttataag ataccctgta ttccctttta tgcgatggct tttatcctga ttatgcatag
     2821 atccggaaaa ttcaaccaac catttatctt tagcaaacga ttattttagc ggattaatag
     2881 tagtagccgg actgcaccac ccatttccat gcagtctgtt tcgtatctga accaattttc
     2941 aagaacatca tcttaccacc atgtgcggga tcgcaggctc tgtaaatttt aacttagaca
     3001 ttccacgtct gaccaaagac ctctggcacc ggggaccgga cgaacaggcc tcctgggcgg
     3061 agaacaatgt acaattgcac catcaccggc tggccatcct ggatattgcc agcggcaaac
     3121 aaccgatgca ataccgggag tttaccatta tttttaatgg cgagatctat aatcacctgg
     3181 acgtgcggaa aaaaatggga ttgcaatgcg tcacccattc cgatacnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnatgc cttaccagca ggtaaaaaaa ttaaccgccg
     3361 gcagttatgc ggtgatcagt acccgggacc tctccgtaaa cgtaacccgc tggtgggata
     3421 ttcatccgtt ttatcagcaa catagtaagg atagttttga acaggccctg gagaaaacgg
     3481 ataccttgtt aaaagcggcc gtgaaacgcc ggattgaatc ctccgatctc gaagtgggtt
     3541 ctttcctgag cggcggtatc gacagcggac tggtaaccgc catggccagt gaatacaata
     3601 accggctcaa aacatttacg gtttcttttg ccggtggtga atatgatgaa gctcccctgg
     3661 ctaaactggt agctgaccgg tatggcaccc aacataatga gatcgtgatc agctttgatg
     3721 gtctgctcga tgatgtggag accattctcc ggaattacgg attgcctttc tttgacagct
     3781 cggccatccc cagctattat gtcagccgcg aagcccgcaa acatctcaag gtcatcctca
     3841 atggagacgg gggcgatgaa atctttggcg gataccgccg gtatgttccc tttgccagct
     3901 atgatttttt caaatcaggc ggactggtaa aaaatctttc cagtcttgtt ttccccttaa
     3961 tgccgatctc gcacgataaa aagaataaat acaattttat ctaccggctg gtcgacctgg
     4021 cccgcaaaga agggctcgat acttatttct cttccaccct ggatattttt gaagaccatg
     4081 aatccgggtt gaaaggcaac cgggattatt ctttacgtag tatacagcaa tcctttgatc
     4141 agatcagtca atcgaagctc agcgggatgc agaaaatgct caacctggat tttgatcata
     4201 tcctggaagc cagtctgctg gtgaaaatgg atattgccac catggcccat tcactcgaag
     4261 gccgcagccc gatgctggca aaagagatac tggaatacat acccacgctg ccggatgcct
     4321 ataaagtaaa aggtacccaa acaaaatacc tgcttcggac cctggccgga aaatacctgc
     4381 ctcccgaact gatccatcaa cccaagcgtg gatttgaaat cccgctcaaa aaatggatcg
     4441 atggcgaact gaaggacctg atcgccagct atatcctggc gcccaatgcg tattgccgca
     4501 gcttcctcca acccggcttt atcgaaaacc tctggaacaa aaaattacaa accggcgctg
     4561 aaaaaagagc gaagatgatc tggtcgttgt ttgcgctgga agtgtggtat aggaaatgtt
     4621 acttgaagaa atgaggcagg cggcccccta atcccccgaa gggggacttt agcctccgaa
     4681 agattgcaga tacttttaat cattgcattg cgtaattgtt tcccgccaag ccaaaaagag
     4741 ttttctataa ttcggagtgt cttaaaatct ttgcgggagc tttttacgcg agagtaaggc
     4801 cccctaatcc cccgaagggg gactttagcc tccaaaagat tgcagatact tttaatcatt
     4861 gcattgctta tttgtttccc gctaagccaa aaagagtttt ctataattcg gagtgtctta
     4921 aaatctttgc gggatagtat taagccagtc aaaaaaataa tgtttcatta aataatttga
     4981 gtcttcaaag tcccccttcg ggggatttag ggggccaata atttgagtct tcaaagtccc
     5041 ccttcggggg gattaggggg ccttctcaaa agatgccaaa gctcctccga atcaccaccg
     5101 cccccatctc cctcaacgtc ctcctgcccg gacaaatgaa atacatgcgg gagcagggat
     5161 ttgatgtgct catggtgagc agtgacggtc ccgaactggc caccgtgctg gaacgcgagg
     5221 gatgccgtca tcatattatt ccaatgacgc ggaagatgag tccttttgcg gacctgcgtt
     5281 gtttatggtt actctatcgt ttgtttctta aagagaaacc ggatatcgtg cattcccata
     5341 cacccaaggc cggattactg gccatgctgg cggcaaaaat ggcaggggtg aaattccgtt
     5401 tacatacaat tgcgggatta cgttatgtca ccaccaaagg cctgagtcgc cgcatcctga
     5461 tcgccatgga gaaactgact ggtatggcgg ccacccatgt atggcccaat agcggatcca
     5521 tgaaagcgtt tatcgaagcc aatcacctgg tggcaacccg taaactcgaa gtgatcgggt
     5581 gggggtccag taacggggtc aacctgcagc gttatcatcc ttcggtgttg aaagaagata
     5641 aactgaatga agtaaaggcc ctggtgaatt atgatccgga tcttttttat tttttatccg
     5701 tcggaagact ggtgcatgat aaaggcatgg atgaattagt aggggccttc gtccgtgtgc
     5761 atgcagtaca tccgcatacc cgcctgatcc tggtgggtgc ttttgaagat gaagtggatc
     5821 ccgtttctga taaagcccgt gaattgatta aaacacatcc ggggattata gcagcgggct
     5881 ggagtgatgc agtggaatat tttatgcagt tttccaatgt gctggtccat ccttctcacc
     5941 gcgaaggatt tcccaatgta ctcatgcaag ccggcgccat gcgttgtccc attatctgct
     6001 ccgccatcga tggcaatacc gatatcgtcc ggcaccgtga aaccggactc ctgtttgacg
     6061 taaagaacga agaagcgctc tttaaccaga tgcaatccgc tattaatgaa cccgataccc
     6121 tgaaagccat ggccgggcaa ctcctgctcc agatacaaac gcattatgat cagcgtgttg
     6181 tgcaatcctt cctgcacgaa aaataccttc agctcctggc tgccggttag atttaccccc
     6241 ctgtatcgaa aaactacgat tttagtaaaa gtactagtac ctgattttcc ggtttagtgg
     6301 cttcattgtt ttgcaagggt gagcgatctg tttattttag ctcatccaaa atccaaatca
     6361 atgaaaacca cattgaaaaa acccgtggcc attgtcggct attccggcca cgcattcgtc
     6421 atcatcgata tcctgctcag tgcgggacga ttggtgactg cctattgcga ctccgaagaa
     6481 aaggagttta atccctatca tttaaagtat ctgggaagag aaggtgatgt aatgcacaaa
     6541 ctgaaggatt atgatttctt cgcttgtgtg ggacataacg gtatccgcga aaaaattcat
     6601 accaatctca gtcagtacct gggcaacccg atcaacgcga ttcatccaag tgcggtgatc
     6661 tctgcttccg ttaaactcgg tgaaggcgtg atgattgcgg ctaatgccac cctcaatcct
     6721 ttagtggaaa tcggtcgtgg agtgatctgc aatacgtcca ccagtattga tcatgaatgt
     6781 atcatcggtg atttcaccca tattgcgccc ggtgccgt
//
LOCUS       DAOPMW010000155         6802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12751_length_6802_cov_7.381058, whole genome shotgun sequence.
ACCESSION   DAOPMW010000155 DAOPMW010000000
VERSION     DAOPMW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_6802_cov_7.381058"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PLU11_08495"
     CDS             <1..1585
                     /locus_tag="PLU11_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HPN59198.1"
                     /translation="MQMIFQVSNDGVAFRYLFQGKTTTLKYITKEHTSFHFYEGARAW
                     LQPKTEAQSGWEHSNPSYEAHYMMDIKTGTPAPGANGWIYPAMFKYGDTWMLLTEAGL
                     GRTYCGTSLQQNSPDNEYKINFPQPAEKFTNGALNPQSVLPWESPWRIIAIGELKTIV
                     ESTLGTDLALPASFTDTSYIKPGRASWSWIMSKDDYIIYKEQKKYIDYAADMGWEYCL
                     VDAAWDKKIGYDSIQILADYAATKKVGLILWYNSAGAWNTVKYTPKDMLLTKESRMKE
                     FGRIHKMGIKGVKVDFFAGDGQSMINYYQDILEDAAANKLLVNFHGATLPRGWHRTYP
                     NLMTTEAVYGYEMITFNQKDADQAGPHMVMSAFARNAFDPMDFTPVSLYKIPGKERRT
                     SPAFELATSVIFLSGIQHFVEGPEGMATVEPEVKKFMQQVPVTWDETIFLDGYPGKSY
                     VVARRSGSKWFVAGINGDSTDLNLQLDFPFVKEAQKGQLFTMDNGVLRAKEYTSALRK
                     GHNHFFLKPNDGFVLVFEE"
     gene            1582..2583
                     /locus_tag="PLU11_08500"
     CDS             1582..2583
                     /locus_tag="PLU11_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPN59199.1"
                     /translation="MKQTLLIAGLLIFAVNVFAQKKAPKPVFDDPVYHGAADPVIVYN
                     KNVKKWWMFYTNRRASITDSTVAWVHGTRIGIAESKDGRKWKYVDTANIQYRPDPGYT
                     HWAPDIIEAKDGWHMYLTYVPGTFTDWNHPRVIVHLQSTDLVNWSYLSTLQLINQKVI
                     DASVYKVNDSLYRLWYNNEKDGKSIWFAESPDLKTWIDKGKAIAARGEGPKVFYWHDQ
                     YFMIVDAWKGMEVYSSTDLLKWTKQKDRILETPGKGKDDQAIGGHCEVIVNNDRAFVY
                     YFTHPGRRKDKPAAKGSFDDKRSVIQLAELEYKDGQIICDRDKPVKIKLQKPRNKGK"
     gene            2580..5456
                     /locus_tag="PLU11_08505"
     CDS             2580..5456
                     /locus_tag="PLU11_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN59200.1"
                     /translation="MKYSVTILLLVLNQLLFAQEISLAGNWRFAMDARDEGIVQQWAS
                     KKLSDQILLPGTMITRNKGEEVGLKTKWTASIYDSSFFFQPRLEKFRQPDNYKVPFWL
                     TPQKYYVGAAWYQRDILIPADWKGKQVNLFLERIHIGSAIWIDGNRIDSFQNSLSVPH
                     VYDLTRFASPGKHTITIRVDNRTRVADVGQDSHSVSDHTQGNWNGIIGRISLQAKSAF
                     HIEDVQIYPLVQQKKIKVRVELQNNSAAAQSVQLNFLIQGKGKKNNSVNAITAQVNPG
                     DTIIHEQLIDMGNELSLWNEFNPFLYSLQVSMKKESQQRKTVFGMREITVKDKSILLN
                     GQKIFLRGDLNNCEFPLTGHPPMDVKSWLNVFTTLKNYGLNHVRFHSWCPPEAAFTAA
                     DELGIYLQPEAPTWPNHGTSVGDGRFIDQYIYDETNRMVKAYGNHPSFLMLAAGNEPA
                     GRNQSKYLADFVHYWKAKDARRIYTGASVATSWPLVPENEYMVKSGARGLNWNSKAPS
                     SMDDYQETMNKYPVPFVQHEQGQWCVFPDFSEISLYTGAYKARNFELFREDMKAQGLL
                     PLAPAFLQASGKLQALCYKMEMERSLRTKDLAGFQLLGLQDFPGQGTALVGVLNAFYK
                     EKGYINETQFRQFCNSVVPLARLPRFTFSNNEEVTAAIELYQYGPADMNNTILDWKLV
                     DQQGSIQQTGSMPAKNYSRGALHEVGSVRFELRGISAPQELQLIVSVRGTVFRNQWSI
                     WVYPAGLPMVEFTDRPDLLVTDTLDDKAVEILNNGGKVFLNLYGKVTKGREVVQAFTP
                     VFWNTSWFKMRPPHTLGLLIDSASPAFRFFPTASHSDLQWWEVVTHSQVMHLEDFSPA
                     MPVLIRPIDTWFMNRNLGLAFEARIGKGRLLITSISLNQNSAVMRQLRFSLYQHLLFA
                     TEWSNTSVSPEQIRALTAEPSRFVFDAYTKDSPDELKPIKNVKQ"
     gene            5484..>6802
                     /locus_tag="PLU11_08510"
     CDS             5484..>6802
                     /locus_tag="PLU11_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN59201.1"
                     /translation="MKKYLLIGILQLTSLLAFAQQKQIQYLSGMDAANTVQWDFFCTA
                     GRNSGEWKKIQVPSCWEQQGYGNYNYGRDYKTNGKNARFYDEKGMYKYSFKVPAAWKG
                     KQVNIVFDGSMTDTEVKINGQSAGAIHQGAFYRFKYDISKLLDYTKTNTLEVTVSKMS
                     SDASVNNAERLADYWVFGGIFRPVFLEAMPANYIDYVGIDAKADGSIAMQVWPKGLTN
                     DAVVVTTITDAANKKLMQVKKRVSKSAENVIVKMGIPGIKTWTAETPNLYTATVVLQD
                     PMGKKLYELTEKFGFRTIEIKYGQGIYINGTQVKFKGVNRHCWWPETARSLSRDQERM
                     DALLLKEMNMNAVRCSHYPPDKYFLELCDSLGIYVLDELAGWQKYYSSKAGEPLVKEL
                     VLRDLNHPSIILWDNGNEGGTNKDLDDDFVKWDFQKRPVIHPHHRPG"
BASE COUNT         1906 a         1521 c         1690 g         1685 t
ORIGIN      
        1 aatgcagatg atcttccagg tctccaatga tggggtggct ttccggtatt tatttcaggg
       61 taagaccacg actttaaagt acatcaccaa agaacatacg agtttccatt tttatgaagg
      121 cgcccgggcc tggttgcaac ccaagaccga agctcagagt ggctgggaac acagtaatcc
      181 gtcttatgaa gcgcattata tgatggatat taagaccggt actcctgcgc cgggggctaa
      241 tggatggatc tatcccgcga tgtttaaata tggggatacc tggatgctgc tcaccgaagc
      301 gggactgggg cgtacttatt gcgggacatc cttacaacaa aattctccgg ataatgaata
      361 caagatcaat tttccacagc cggcggagaa attcaccaat ggggccttga atccacagtc
      421 cgtactgccc tgggaatcac cctggcgaat cattgccata ggggagttaa aaacaattgt
      481 tgaatctacc ttgggtactg atctcgcctt gcctgccagt ttcaccgata catcgtatat
      541 caagcccggc cgggcttcct ggagctggat tatgagcaag gatgattata tcatttataa
      601 agaacagaaa aaatacatcg actatgcggc tgatatgggc tgggagtatt gtctcgtgga
      661 tgccgcctgg gataaaaaaa tcggctatga cagtattcag atccttgctg attatgcggc
      721 taccaaaaaa gtaggcttga tcctctggta taattcagcc ggagcctgga acacggtgaa
      781 gtatacgccg aaggatatgt tgctcaccaa ggaaagccgc atgaaggaat ttggtcgtat
      841 tcataaaatg gggatcaaag gagtaaaggt tgatttcttt gcgggtgatg gacagtctat
      901 gatcaattac taccaggata tactcgaaga tgcggccgcg aataaattat tagtcaattt
      961 tcatggggcc actttacccc gtggctggca ccgcacttat cccaacctca tgaccactga
     1021 agcggtctat ggttatgaga tgatcacctt taatcagaaa gatgccgatc aggctggtcc
     1081 acatatggtg atgagtgcct ttgcccgcaa tgcctttgat ccgatggact tcacccccgt
     1141 cagtctgtat aagatacccg ggaaagagcg tcgtaccagt cctgctttcg aactggccac
     1201 ctcggtgatc ttcctctccg ggatccagca ctttgtggaa ggaccggaag gcatggctac
     1261 ggtagaacct gaagtgaaga aattcatgca gcaggtaccg gttacctggg acgaaactat
     1321 tttccttgat ggttatcccg gtaagtctta tgtggtggcc cgtcgctccg gcagcaaatg
     1381 gtttgtggcc gggatcaatg gcgacagtac cgatctgaat ctccaactgg attttccctt
     1441 tgtaaaagaa gcgcagaaag ggcagttgtt caccatggac aatggggtac tgcgggccaa
     1501 agaatatacg tccgccttac gtaaagggca taatcacttt ttcctcaaac ccaatgacgg
     1561 gtttgtgctc gtatttgaag aatgaaacaa actctcttaa tagccggttt attaattttt
     1621 gcggtcaatg tgtttgcaca aaagaaggcc cctaaaccgg tatttgatga tcccgtgtat
     1681 catggcgctg ccgatccggt gatcgtctat aataagaatg tcaagaaatg gtggatgttt
     1741 tacaccaacc gtcgtgcatc tattacagac tctaccgtgg cctgggtaca tggcacccgt
     1801 atcggaatag ctgaaagtaa agacggtcgt aaatggaaat acgttgatac cgccaatatt
     1861 caatatcgtc ccgatcccgg ctacacccat tgggcaccgg atatcatcga agcaaaggat
     1921 ggctggcata tgtatcttac ttatgtaccc ggcacgttta ccgattggaa tcatccaagg
     1981 gtcattgtac atctccaaag tacggacctt gtcaactggt catacttgtc aacgctgcaa
     2041 cttataaacc agaaagtaat cgatgcctcc gtttacaaag taaatgattc attatatcgc
     2101 ttgtggtaca ataacgaaaa ggatggcaag agcatctggt ttgccgaatc acctgacctt
     2161 aaaacctgga tcgataaagg caaagccatt gccgccaggg gagaaggtcc caaagtattt
     2221 tactggcacg atcaatactt catgatcgtg gatgcctgga agggcatgga agtgtacagt
     2281 tccactgatt tattaaagtg gaccaaacaa aaagaccgta ttctcgaaac acccggcaag
     2341 ggcaaagacg atcaggccat cggtggtcac tgcgaagtca ttgtaaataa tgatcgagcc
     2401 tttgtgtatt atttcacgca tcccggtcgg cgcaaagaca aaccggcagc aaaaggttca
     2461 ttcgatgata aacggagtgt gatccagctg gcggaactgg aatacaagga tggacagatt
     2521 atttgtgacc gggataagcc cgtgaagata aaattgcaaa agccccggaa taagggtaaa
     2581 tgaaatattc agtaaccata ttgctgctgg tactcaatca actgcttttt gcgcaggaga
     2641 tatcactcgc tggcaactgg cgtttcgcta tggatgcacg ggatgaagga atagtgcagc
     2701 aatgggccag caaaaaacta tcggatcaga ttttgttacc cggaaccatg atcaccagga
     2761 ataaagggga agaggtcggg ctaaagacaa aatggaccgc cagtatttac gacagttctt
     2821 ttttctttca acccagattg gaaaaattcc ggcaaccaga taattataaa gtccccttct
     2881 ggctcacgcc acagaaatat tatgtgggtg cggcctggta tcaacgggat atcctgattc
     2941 cggcagactg gaaaggcaaa caagtgaacc tgtttttaga acggatacat attggctctg
     3001 ctatctggat cgatggcaac cggatcgata gttttcagaa ctcactatct gttccccatg
     3061 tatatgatct gacccgcttt gcttcgccgg gcaaacatac cattaccatt cgggtcgata
     3121 accggaccag ggtggccgat gtgggacagg attcacacag tgtatccgat catacccagg
     3181 gcaactggaa cggcatcatt ggtcgcatca gtctgcaggc aaagtctgca tttcatattg
     3241 aggatgtaca gatctatcca cttgtacaac aaaagaagat taaagtacgg gtggaactac
     3301 agaataattc agctgctgcc caatccgttc aattgaactt cctgatccag ggaaagggga
     3361 agaaaaataa ctcagtcaat gccatcactg cccaggtgaa tcccggagac acaatcattc
     3421 atgaacagct gatcgatatg ggtaatgagc tgagtttgtg gaatgagttt aatccctttt
     3481 tatattccct gcaggtatcg atgaaaaagg aaagtcagca acgcaaaact gtatttggga
     3541 tgcgggaaat aacagtgaaa gacaaatcca tcctgctcaa tggacaaaag attttcttaa
     3601 gaggtgatct gaataactgt gagtttccgc tgaccggtca cccaccgatg gatgtgaaga
     3661 gctggctgaa tgtatttacg accttgaaga attacggact caatcatgtg cggtttcatt
     3721 cctggtgtcc gcccgaagcg gcctttaccg ctgctgatga actcggtatt tatttacaac
     3781 ccgaagcacc cacctggccc aatcatggga catcggtagg cgatggtcgt tttattgatc
     3841 agtatatcta tgacgaaaca aaccgaatgg taaaagccta tggcaatcat ccatctttcc
     3901 tgatgctggc tgcggggaat gagcctgccg gaaggaatca atccaaatac ctggctgatt
     3961 ttgtacacta ctggaaggca aaagatgcac gacgcattta taccggagcc tctgtagcta
     4021 ccagctggcc gttggtaccg gagaatgagt atatggtgaa gtcaggtgca agaggactca
     4081 actggaacag caaggcccct tcctccatgg atgattacca ggagacgatg aataaatacc
     4141 cggttccttt tgtacagcat gaacagggac agtggtgtgt attccccgat ttctcagaga
     4201 tcagtttata taccggtgcg tataaagccc gcaattttga attgttccgc gaggatatga
     4261 aggcacaggg attattacca ctggctcctg ctttcctgca agcttcgggt aaactccagg
     4321 ccctttgtta taagatggaa atggagcgat cgctgcgcac gaaagacctg gccggattcc
     4381 agttactcgg tttacaggat tttcccggac agggtacggc actggtaggg gtactgaacg
     4441 cattttataa agagaagggc tatatcaatg agacacagtt cagacagttc tgcaatagtg
     4501 tggtgcccct ggcccgtctg ccacgtttta cattctcaaa taacgaggaa gtgacagccg
     4561 ccattgaatt gtatcagtat ggtccggcag atatgaacaa taccattctg gactggaagc
     4621 tggtcgatca gcagggaagc atccaacaaa ctggcagcat gccagcaaaa aattacagcc
     4681 ggggtgcttt acatgaagtg ggttcggtcc ggtttgagtt gcgaggaatc agtgctccgc
     4741 aggaactgca attgatcgtt tctgtgagag ggacagtgtt ccgtaaccaa tggagcatct
     4801 gggtgtatcc tgccggattg cccatggtgg aatttacaga ccgtccggat ctgctggtca
     4861 cagataccct ggatgataag gcagttgaaa ttttaaataa cgggggtaaa gtattcctga
     4921 acctgtatgg aaaggttacc aaagggcgcg aagtggtaca ggctttcaca ccggtattct
     4981 ggaatacctc ctggttcaaa atgcggccac cccatacact cgggctgctg attgattccg
     5041 ccagtcctgc attccgtttt ttccctacgg cttctcatag tgaccttcag tggtgggagg
     5101 tggtcaccca ctcacaggtc atgcacctgg aagatttttc accggccatg ccggtactca
     5161 tacggcccat tgatacctgg tttatgaaca ggaacctggg gctggcattt gaagcccgga
     5221 tcgggaaggg acgattactg ataaccagta tttcactcaa tcaaaattca gcggtgatgc
     5281 gacagttgag gttttccctg tatcagcacc tgttgtttgc aacggagtgg agtaatacca
     5341 gcgtaagtcc cgaacagatc cgtgccctga cggctgaacc atcccgcttt gtatttgatg
     5401 cctataccaa ggacagtccg gatgaattga agcccataaa gaacgttaag caataattga
     5461 gtgaagcaat aaatatacat gacatgaaaa agtatttact gatcggtatc ctgcagttga
     5521 ccagcttact ggcgtttgcc cagcaaaaac agattcaata cctctccggt atggatgccg
     5581 ccaataccgt gcaatgggat ttcttctgta ccgccggtcg caacagcggc gaatggaaaa
     5641 agatacaagt gccttcctgc tgggaacaac agggctatgg caattataat tacggccgtg
     5701 attataaaac caacggaaag aatgcccggt tttatgatga aaaggggatg tacaaatatt
     5761 ccttcaaagt accggccgcc tggaaaggca agcaggtgaa tattgtcttc gatggcagca
     5821 tgaccgacac cgaagtaaag atcaacggcc aatccgccgg tgccattcac cagggagctt
     5881 tttatcgctt caagtacgat atcagcaaat tactggatta tacaaagacc aatacactgg
     5941 aagtaaccgt cagcaagatg agcagtgatg cctcagtcaa caacgccgag cggctggccg
     6001 actattgggt ctttggtggc atcttccgtc cggtgttcct ggaagccatg cccgccaatt
     6061 atattgacta tgtaggtatc gatgccaaag cggatggtag tatcgctatg caggtctggc
     6121 cgaaagggtt gaccaatgat gcagtagtgg taaccaccat tacagatgcc gcgaataaaa
     6181 agctgatgca ggtaaaaaag cgcgtgtcta aatcagcaga gaatgtgatt gtgaaaatgg
     6241 ggattccggg tataaagacc tggacagctg aaacgcccaa tctgtatact gccaccgtgg
     6301 ttttacagga tcccatggga aagaaattgt atgaactcac agagaaattc ggattcagaa
     6361 ccattgaaat aaaatacggg caaggcattt atatcaacgg cacccaggta aaatttaaag
     6421 gagtcaaccg tcactgctgg tggcctgaga ccgcccgttc cctcagtcgg gatcaggaaa
     6481 gaatggatgc cttactgttg aaagaaatga acatgaatgc cgtccgttgt tcgcattatc
     6541 ctcccgataa atatttcctg gagctttgtg atagtctggg tatttatgta ctggatgaac
     6601 tggccggctg gcagaagtat tattcttcca aggccggtga gccactggtc aaagaactgg
     6661 tgctgcgtga cctgaaccat ccttctatta ttttatggga taatggcaat gagggtggca
     6721 ccaataaaga tctggatgat gattttgtca aatgggattt tcaaaaacgt cccgtgatcc
     6781 atccccatca ccgtccgggt aa
//
LOCUS       DAOPMW010000156         6775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12818_length_6775_cov_11.182292, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000156 DAOPMW010000000
VERSION     DAOPMW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12818_length_6775_cov_11.182292"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="rfbA"
                     /locus_tag="PLU11_08515"
     CDS             <1..726
                     /gene="rfbA"
                     /locus_tag="PLU11_08515"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPN59202.1"
                     /translation="GIREVLIITTPEDNPSFIRLLGDGSKVGCRFEYAIQEVPNGLAQ
                     AFVIGEKFIGDDKVALVLGDNIFYGTRMGKQLKDLAHVEGGYVFAYQVADPERYGVVE
                     FDEHYNALSIEEKPAKPKSNFAVPGLYFYDNSVVKIAKELKPSPRGEYEITDVNKEYL
                     RRGDLKVALLDRGTAWLDTGTFDSLSDASEFVRVIEKRQGNKIGCIEEIAFRNGFINR
                     EQLDAIAVELAKSGYGEYLKRII"
     gene            737..2062
                     /locus_tag="PLU11_08520"
     CDS             737..2062
                     /locus_tag="PLU11_08520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPN59203.1"
                     /translation="MKIAVVGTGYVGLVSGTCFADTGNDVTCIDIDENKVRKLNAGEI
                     TIYEPGLEKVFSRNLKEGRLHFTTSLEKGIEGAAILFLALPTPPGEDGSADLKYILGV
                     AADLGRLFKPGDYKVIVDKSTVPVGTADKVTATILAAASTDISGCFDVVSNPEFLREG
                     AAVEDFMKPDRVVIGSSSERARKMMQQLYEPYVRQGNPIIFMDEKSAELTKYAANSFL
                     AAKISFMNEIAVLCEKLGADVDMVRRGIGTDFRIGKHFLYPGLGYGGSCFPKDVQALA
                     KSAADAAYDFKILNAVMQVNAGQKSFFFEKISRYYGDNLKGKKFAVWGLAFKPNTDDI
                     REAPALEIITRLTRMGATVTAFDPEAMPNVRREFKGELAYADNQYEALKGADALVICT
                     EWNEFRTPDFNRIAGQLAAKVIFDGRNLYDTTQMKAHGFYYESIGRTAS"
     gene            2095..2859
                     /locus_tag="PLU11_08525"
     CDS             2095..2859
                     /locus_tag="PLU11_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59204.1"
                     /translation="MKKKITRIAPWLFILFLLIFLLFNASPGITEKDEQYLVAFLKEW
                     NIELDKKQIHRNQENEVAFISRLQDSVLHSISHFTISFDSAGKVENYFHQRKGLCFDR
                     SLLLEKFCLLAGFKVRHLYIFFNGDGQKPERSDFFKKGLTSHAMFEVKTSQGWMAVGT
                     NSNWLGQDIEGSPLSLKEIRSRLGKNKLELKKSFTVGLPFYDELRQKDAFRFVYGIYS
                     RHGQFLKSRPLETWLNSIGLKSKLPDYNINMLFSNL"
     gene            2889..3863
                     /locus_tag="PLU11_08530"
     CDS             2889..3863
                     /locus_tag="PLU11_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN59205.1"
                     /translation="MKRILITGAAGFLGSHLCDKFIGKGYEVIGMDNLITGNLKNIEH
                     LFPLKEFSFYHHDVSKFIHVPGHLDYILHFASPASPIDYLKIPIQTLKVGSLGTHNCL
                     GLALNKKARILVASTSEVYGDPQVHPQTEDYWGNVNPVGPRGVYDEAKRFQEAMTMAY
                     HTYHGLETRIIRIFNTYGPRMRLNDGRALPAFIGQALRGQDLTVFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYAQPVNIGNPDEISLKDFAEEIIKLTGTSQKIIYKPLPTDDPKQR
                     QPDITKAKAILGWEPTVSRAVGLKATYEYFKSLPEEEIWADNLEFKKG"
     gene            4214..4828
                     /locus_tag="PLU11_08535"
     CDS             4214..4828
                     /locus_tag="PLU11_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPN59206.1"
                     /translation="MLHFETIDTPTLELMNKIMAVPLFSPFRLVGGTALALQTGHRKS
                     VDLDFFGPCLLEEGELTEELSNFGSITWLNKLKNIKSLLVNGIKVDFVNYSYPWLSGL
                     IEEENVRIASKKDIAPMKLTAITGRGAKKDFIDLYFLLQEFTLAEMISLYEEKYKDGS
                     TFMVLKSLVYFDDAENDEMPEMLKPITWQDIKETIVKENKNYLE"
     gene            4933..5508
                     /locus_tag="PLU11_08540"
     CDS             4933..5508
                     /locus_tag="PLU11_08540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPN59207.1"
                     /translation="MSSSPSIHPSSVADTGAIIGAGTKIWHFSHIMPEAVIGENCVIG
                     QNVFIANKVVIGNGVKVQNNVSLYEGVICGDEVFIGPSAVFTNVINPRSAIERKNEFR
                     TTRIGKGATIGANATILCGIEIGTYAFIGAGAVVTKDVPAFALMTGNPARQTGWMSEA
                     GEKLELKEGLAVCKVSGMEYQLENGKLIRKV"
     gene            5600..6493
                     /locus_tag="PLU11_08545"
     CDS             5600..6493
                     /locus_tag="PLU11_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59208.1"
                     /translation="MKNFLLIGAAGYIAPRHMRAIRETGNNLLAALDPHDSVGVLDNN
                     FPGAQFFTEFERFDRYCDKVKRKGTAIDYLSVCSPNYLHDAHIRFGLRIGADVICEKP
                     VVLNPWNVDALLEMEKETGRKVYSILQLRLHPAIMALKNKIDTTNHTAKYQVDLQYIS
                     ARGNWYLNSWKGEEAKSGGIATNIGLHFFDMLLWIFGDVTRLNVTTYSPVKTAGTLEL
                     EKAQVNWTMSIDENDLPVSVREKGQRTFRAMTINGDPLDFSEGFEELHTKSYEAILSG
                     HGIPLEETRKGIQLVHDIRRV"
BASE COUNT         1951 a         1485 c         1634 g         1705 t
ORIGIN      
        1 ggcatccgtg aagttttaat tatcaccaca ccggaagaca atccttcttt tattcgcctg
       61 ctcggtgatg gcagtaaagt gggttgccgt tttgagtacg cgatccagga agtgcctaac
      121 ggactggcac aggcttttgt gatcggcgaa aaatttatcg gggatgacaa agtagccctg
      181 gtactcggtg ataatatttt ctatggtacc cggatgggta agcagctgaa agacctggcc
      241 catgtggaag gcggttatgt gtttgcctac caggtggccg atccggagcg ttacggggta
      301 gtggaatttg acgaacacta caatgccctc agtattgaag aaaaacccgc taagcccaaa
      361 tccaattttg cggttcccgg cttatacttc tatgacaaca gtgtagtgaa aatcgcgaag
      421 gaattaaaac cctctccccg cggcgaatac gaaatcaccg atgtaaataa agaatacctc
      481 cgccgcggcg atctgaaagt ggccctgctc gacaggggta ccgcctggct cgataccggc
      541 acctttgatt ccctgagtga tgccagtgag tttgtacgcg tgattgaaaa aagacagggc
      601 aacaagattg gctgtattga agagatcgcc ttccggaacg ggtttatcaa ccgtgaacaa
      661 ctggatgcga ttgcggtgga attagcgaag agtggatatg gagaatacct gaaacgaatc
      721 atttaaaacc tgactcatga aaattgctgt tgttggtacc ggatatgtcg gactggtaag
      781 tggtacctgt tttgccgata ccgggaatga tgtgacctgt attgatattg acgaaaataa
      841 agtacgtaag ctcaatgccg gagaaattac catctatgaa cccggactgg agaaagtatt
      901 ttccaggaac ctaaaggaag gacgacttca tttcactact tcgctggaaa aaggaatcga
      961 aggtgcggcc atcctgttcc tggccctgcc tacccctccc ggtgaagatg gcagtgccga
     1021 tctgaaatat attctgggcg tggccgccga tctgggcagg ctgttcaaac ccggagatta
     1081 taaagtaatc gtggataaaa gcaccgtacc agtgggtacg gccgataaag tgacggcaac
     1141 gattctggcc gccgcttcaa cggatatttc cggatgtttt gacgtggtca gtaaccccga
     1201 atttttaagg gaaggggccg ccgtggaaga ttttatgaaa ccggaccgcg tggtgatcgg
     1261 cagcagttca gagcgggccc gtaaaatgat gcagcagctc tatgaaccct atgttcgtca
     1321 gggcaacccc atcatcttta tggatgagaa aagtgcggag ctgaccaagt atgccgccaa
     1381 tagtttcctg gccgccaaga tttcgttcat gaacgaaatc gcggtcttat gcgaaaaact
     1441 cggtgctgat gtggatatgg tacgccgtgg tattggtacc gatttcagaa tcggcaaaca
     1501 ttttctttat cccggattgg gatacggggg cagctgtttc ccgaaagatg tacaggccct
     1561 tgctaaatcc gccgcggatg ccgcttatga ttttaagata ctgaatgcgg tgatgcaggt
     1621 gaatgccgga cagaaatcat ttttctttga aaagatcagc cgctattatg gcgataacct
     1681 gaaagggaaa aaatttgccg tatggggact ggcttttaaa cccaataccg atgatatccg
     1741 agaggcacca gccctcgaaa tcatcacccg gctcacccgg atgggcgcca ccgttaccgc
     1801 ttttgatccg gaagcgatgc cgaatgtgag aagggaattt aaaggggaac tggcctatgc
     1861 cgataaccag tatgaggccc tcaaaggcgc cgacgccctg gttatctgta ccgagtggaa
     1921 tgaattccgg acaccagact ttaaccggat cgccggtcag ctggctgcca aagtaatttt
     1981 tgatggccgc aacctctatg ataccacgca gatgaaagcc catggttttt attatgaaag
     2041 tatcggcaga acggccagtt gagccggcaa gtaataacct tattcctgtt taaattgaaa
     2101 aagaaaatca ccaggattgc cccctggctg tttattctgt tcctccttat ttttttactg
     2161 ttcaacgctt ccccgggcat caccgaaaaa gatgaacaat acctggtggc ctttttgaaa
     2221 gagtggaata ttgaactgga taaaaagcaa atacaccgca accaggaaaa tgaagtagcc
     2281 tttatttccc ggttgcaaga tagtgtcctg cacagcatca gccatttcac catctcgttc
     2341 gatagtgccg ggaaagtgga aaattatttt catcaacgaa aaggactctg tttcgaccgc
     2401 tccctgttgc tcgaaaaatt ttgcttgctg gcagggttta aagtccgtca cctttatatc
     2461 ttctttaatg gggatgggca aaaacccgaa cgaagtgatt ttttcaagaa gggccttacc
     2521 tcgcatgcca tgtttgaagt taaaaccagt cagggctgga tggccgtagg caccaacagt
     2581 aactggctgg gacaggatat agaagggtca cctttaagtc ttaaagaaat caggagccgg
     2641 ttgggaaaaa acaaactgga attgaagaag tcatttacgg tgggacttcc tttttatgat
     2701 gaactccggc agaaagatgc cttccgtttt gtgtatggca tatactcccg gcatggacag
     2761 ttccttaaat cccgtcccct cgaaacctgg ctgaattcga tcggcttaaa aagcaagctc
     2821 ccggactaca atatcaatat gcttttttcc aacctctgaa aatgaccgga atcatctaaa
     2881 taaaaaaaat gaaacgcatt ttaatcaccg gggcagccgg ctttctcggt tcccatctct
     2941 gcgataaatt tatcgggaaa ggatatgaag tcatcgggat ggataacctg atcaccggta
     3001 acctgaagaa tattgagcat cttttcccgt tgaaggaatt cagtttctat catcatgatg
     3061 tcagtaaatt cattcatgtg cccggtcacc tggattatat cctgcacttc gcttccccgg
     3121 ccagtccgat cgattacctg aaaatcccga tccagacact gaaagtggga tcactgggaa
     3181 cgcataactg cctgggactt gccttaaata agaaagcccg cattttagtg gccagtacca
     3241 gtgaagtgta tggcgatccg caggtacatc ctcagacgga ggattactgg ggaaatgtaa
     3301 atcccgtagg tccccgtggt gtatacgatg aagccaaacg tttccaggaa gccatgacca
     3361 tggcctatca tacctaccac ggactcgaga cccgcattat ccggatcttt aatacctatg
     3421 gcccgcgtat gcggctcaat gacggtcgtg ccctcccggc ctttatcggg caggcgctcc
     3481 gcggacagga cctgaccgta ttcggggatg gcagccagac ccgcagcttc tgctatgtgg
     3541 atgacctggt ggaagggatc taccggttac tattaagcga ttatgcccag cccgtcaaca
     3601 tcggcaaccc cgatgaaatc agcctgaaag actttgcgga agaaattatc aaactgaccg
     3661 gcaccagcca gaaaattata tataagccgt tgcccacaga cgatcccaaa caacggcaac
     3721 cggatatcac caaagccaaa gccatactcg gttgggaacc gacggtctcc cgcgccgttg
     3781 gactgaaagc cacgtatgaa tattttaagt cgttgccgga agaggaaata tgggcggata
     3841 atctcgaatt caagaaaggt tagttgttta gctgtttagt cgtttagcgg aacaaacgcc
     3901 taaacaacta aacgtctaaa catctaaacc ttactattag ctacgtcaat atgcataacc
     3961 ctaatcccaa aatagctgat ttttcttccc atcttttttg ggatgtagat agagacaaac
     4021 ttgattggga attaaataaa gcccaaatcg taaaaagagt tttggaatat gggttgttat
     4081 gcgattggga attaattcat caacagtatg gaataaagca attaggagag atatgtaagg
     4141 atttgcgtgc attggatgag cggtcacttg gttttatctc tgttttaaca ggaattccta
     4201 aagaatcttt tcgatgttac attttgaaac aatcgacacc ccgacactgg aattgatgaa
     4261 taaaatcatg gctgttcctt tattctcgcc attcaggctg gtgggaggaa cagctttggc
     4321 tttacaaacc ggtcacagaa agtcggttga tcttgatttt tttggtcctt gtttattgga
     4381 agaaggggag ctgactgagg aactatccaa tttcggtagt ataacatggc tgaataaatt
     4441 aaaaaatata aaaagcctgt tggtaaatgg gatcaaagtc gattttgtaa attattcata
     4501 tccctggcta agcggtttga tagaggagga aaatgtccgg atcgcttcaa aaaaagatat
     4561 tgcccccatg aaactaaccg ccattactgg caggggggca aaaaaggatt ttatagatct
     4621 ttatttcctt ttacaggaat ttacgctggc agagatgatc agcctgtatg aggaaaaata
     4681 taaagacgga tcaacgttca tggtcttaaa gagccttgta tactttgatg atgctgaaaa
     4741 tgacgagatg ccggaaatgc ttaagcccat tacctggcag gatattaaag aaactatagt
     4801 aaaagagaat aaaaattacc tggaataaaa cgaggacata aaatcagtta agaatattgc
     4861 acttaaagct tttttacttt ttacttttta attttgactt ttaaaaccca cttcatttaa
     4921 taacctcccc cattgtcttc ctctccctcc atacatccct catccgtagc agacactgga
     4981 gccatcatcg gagcaggtac caaaatctgg catttctcgc atatcatgcc ggaagcggta
     5041 atcggggaaa actgtgtgat cgggcagaat gtctttattg ccaataaagt ggtgatcggg
     5101 aatggggtga aagtgcagaa caatgtatcg ctgtacgaag gtgtgatttg tggggatgaa
     5161 gtgtttattg gtccttccgc ggtattcacc aatgtaatta atccacgcag tgcgattgaa
     5221 cgaaagaatg aattcaggac aacaagaatc gggaaggggg ccactatcgg tgccaacgca
     5281 accattctct gtggaataga aattggaacc tatgccttta tcggtgccgg tgccgtggta
     5341 acgaaagatg ttccggcctt tgccctgatg accggtaatc ctgcccggca aacaggctgg
     5401 atgagtgaag cgggggagaa gctggagctg aaggaaggat tagcggtttg caaagtgagt
     5461 ggaatggagt atcaattaga aaatggaaaa ctcataagaa aggtttagtt gtttgtcgtt
     5521 tagatgttta gaacatacta agcagatctt ccgctaaacg actaaacgac aaacgtctaa
     5581 acttattaaa ctcttcccct tgaagaattt tcttcttata ggcgccgctg gttacattgc
     5641 tccccgtcac atgcgggcga tcagggagac gggtaataac ctgctggcag ccctggatcc
     5701 gcatgattcg gtgggtgtat tggataataa ttttccgggg gctcagtttt ttacagaatt
     5761 cgaacggttt gaccggtatt gtgataaagt aaaacggaag gggacggcga ttgattacct
     5821 gtccgtttgt tcgcccaact acctgcatga tgcacatatc cgctttggct tacggatcgg
     5881 ggccgatgtg atttgtgaaa agccggtggt gctgaatccc tggaatgtgg atgccctcct
     5941 ggaaatggaa aaagaaacag gaaggaaagt gtacagtatc ttgcaactgc gcctgcatcc
     6001 ggcgatcatg gccttaaaga ataaaatcga tacaacaaat catacagcga agtaccaggt
     6061 cgacctgcag tatatcagcg cccgcggcaa ctggtacctc aacagctgga aaggtgagga
     6121 agcaaaaagc ggcggcatcg ccaccaatat cgggctccat tttttcgaca tgctcttatg
     6181 gatattcggc gatgtaaccc ggctgaacgt gaccacatac agcccggtaa aaaccgccgg
     6241 caccctggaa ctggaaaagg cgcaggtgaa ctggaccatg agtattgacg aaaacgacct
     6301 gccggtttcg gtccgggaaa aaggacaacg cacattccgg gccatgacca tcaacggcga
     6361 cccgctcgat ttcagcgaag gctttgaaga attacataca aagtcctacg aagccattct
     6421 ctccggtcat ggtattccgc tggaggaaac aaggaaagga atacaattag tacacgacat
     6481 aagaagggtt tagacgttta gttgtttaga tgtttagcgg accattttgt aaggctgaaa
     6541 actattaaaa gcgggtaaat gaaaatgaat aatcaattct ttagtgaatt ttccgctaaa
     6601 cgactaaacg tctaaacgac taaacaccaa gccttaaact tttcgatcaa tagtgagatt
     6661 gcaaatttta aaccactacc gctaaacgac taaacaacaa acaactaaac tctctctccc
     6721 ccattcaacc tcttcaaact atgaataaaa cagcactcat caccggtatc accgg
//
LOCUS       DAOPMW010000157         6759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12863_length_6759_cov_9.917363, whole genome shotgun sequence.
ACCESSION   DAOPMW010000157 DAOPMW010000000
VERSION     DAOPMW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12863_length_6759_cov_9.917363"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1852)
                     /locus_tag="PLU11_08550"
     CDS             complement(377..1852)
                     /locus_tag="PLU11_08550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPN59209.1"
                     /translation="MKKILVANRGEIALRIMRTIRKMGIKSVAVYSDADRHAPHVLFA
                     DEAVHLGAAPSSQSYLNGDKIIAISKELGVDGIHPGYGFLSENADFAQKVTDAGIEFI
                     GPTPEAMRVMGSKLAAKDSVKKYNIPMVPGIDHAIEDVNEAIETANKIGYPILIKASA
                     GGGGKGMRIVERADDMQEQMERAISEAKSSFGDGSVFIEKYVTSPRHIEVQVVADKYG
                     NVIHGLERECSIQRRHQKVVEETPSAVVTPEMRDRIGEAAVMVAKSCNYVSTGTVEFL
                     VDDKLNFYFLEMNTRLQVEHPVSEMIIGLDLVEQQIRIARGEKLSVTQDDIKPNGHAI
                     ELRVYAEDPLNNFLPSIGTLTKYQKPVGEGIRVDDGYEEGMTIPIYYDPMIAKLVVHG
                     KTRTEAIQKMLQAIKDYKIEGVATTLPFGTFVFEHDAFFSGNFDTHFVKNYYTPEKIK
                     EKQKANAEVAALMALKYWQEQQKKLSPVDHKSTSWKRRLAH"
     gene            complement(1874..2365)
                     /locus_tag="PLU11_08555"
     CDS             complement(1874..2365)
                     /locus_tag="PLU11_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPN59210.1"
                     /translation="MSESTKTFKVKANDFVFFFDQAAIDAADFFKKSPEEFNLIMDHR
                     SVNARLLEADINGKKIKLEVDGESFDMEIKDPLDQMLDSMGFSSASTKHIKEIKAPMP
                     GLVLEIAVKEGQEVKEGDRILILEAMKMENSIMIQSNAVIKRIAVTTGQAVDKGQVLV
                     ELE"
     gene            2752..3153
                     /locus_tag="PLU11_08560"
     CDS             2752..3153
                     /locus_tag="PLU11_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPN59211.1"
                     /translation="MRQRQAAAQEAEKGQQYGNRPAFNRSMQRAAVNIYKTANSYEML
                     VFAPGRIREHFHLDVNGQELSVSYTPPEGFPRPEWVLREYSRGGFKRSFTLDESIDKN
                     AITAKYADGVLQVSLPLLAEKQSLKKEIVIQ"
     gene            complement(3163..3771)
                     /locus_tag="PLU11_08565"
     CDS             complement(3163..3771)
                     /locus_tag="PLU11_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPN59212.1"
                     /translation="MSSHPRPYYLVNAITLYRIVTVPVLLWLLFTGQYPLFKWGVALS
                     FFTDLIDGFLARRFKVTSIAGTKLDSIGDDLTVAVALTGLIIIFPEFVKTHFTILMIL
                     AGVFLAQLSYAFYRYGRMSNFHTWLAKTAALLQGCFLILTFFTGHPSVVFFYIATGIT
                     LLELIEEIILVHFLPEWKANVHSIFHAQSDKKKNGLKEQPVR"
     gene            complement(3856..4497)
                     /locus_tag="PLU11_08570"
     CDS             complement(3856..4497)
                     /locus_tag="PLU11_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPN59213.1"
                     /translation="MKKEIGENVYIPVGSVTLEGRLEVPENADRIIIFSHGSGSSRFS
                     PRNKYVAELLNDKGMATLLVDLLTSRESIANSARFDISLLTQRLAAVTHWIQAQPVFR
                     NYQIGFFGASTGAASALGAASLMPGIVNAVVSRGGRPDLALLALPQVKSPVLLITGSL
                     DDEVTELNQQALERLTCKKELVVIEGATHLFEEPGKLQEVAEIAGEWFRKHLS"
     gene            complement(4769..5515)
                     /locus_tag="PLU11_08575"
     CDS             complement(4769..5515)
                     /locus_tag="PLU11_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPN59214.1"
                     /translation="MTSDFSWLSRTSLLIGEEALQRLAQSHVMVVGLGGVGSYAAEFI
                     ARSGVGKMTIIDGDVVDPSNRNRQLPALATNHGESKALIMAERLKAINPELELDVIRE
                     FINPAMVEQQLLHRPSYIIDAIDSITPKITFIKLAFESGLSMVSSMGAGAKLDPTRLQ
                     VVDISETYNCPFAQQVRKQLKRQYGIRKGIKAVFSPEEPIKESLMLTDGKNYKKSAYG
                     TISYLPAVFGAVAASVVIRDLMLPPKKKKI"
     gene            complement(5512..6165)
                     /locus_tag="PLU11_08580"
     CDS             complement(5512..6165)
                     /locus_tag="PLU11_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPN59215.1"
                     /translation="MYINIHSHKPPGTGEWVLRNLNTPDEPEPLPGLYSMGIHPWYIQ
                     AADWENQLCSLQQKSTHDQVLAIGECGLDKVCATPFSLQLQVFRAQVEWANSIGKPLV
                     IHCVRAWEEVLASLEEATIRVPVLFHGYNKNSQLARRITGKGYYISLGKALNRPGIQE
                     VLRSVPATRFFLETDDAELSIGEIYQQAAKALSIDHNSLVLQIQKNAETVFGNAVQL"
     gene            6365..6748
                     /locus_tag="PLU11_08585"
     CDS             6365..6748
                     /locus_tag="PLU11_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HPN59216.1"
                     /translation="MATKKKAAKKAAAKKPVKKAAAKKPAAKKPAAKKAAKPAKKKSA
                     RKPNAAFMKALTPSAALAAVVGAKPLPRTEAVKKIWAYIKANKLQDAKNRRNVNTDAK
                     LKVVFGNKAQVSMFEIAKFLGKHLS"
BASE COUNT         1756 a         1637 c         1530 g         1836 t
ORIGIN      
        1 cccgttcgcg ggccgtcagt ttacctttct tatgctgggc ctcgatccgt tttatgccgc
       61 caccgagttt accttcttcc agtttttccc tgagtatttc aactttagat ttcattgttg
      121 taggttttgt gttttaggtt tataccttaa ttaaccattt acttttttct acttcaccct
      181 tcgacatgcc tgcctgtccg gtaggcaggc tcccaccttc gctacgctac ggtcggcagg
      241 cagggtgaca acagctcttg tccttctgag acggtgttca tgtcaccctg agcttgccga
      301 agggttgccg aagggcgacg aagagagtgc cctttcttgg tcatggttcg acaagctcac
      361 catgacaaga gccgccctaa tgcgccagtc tccttttcca actggtggat ttatgatcca
      421 cggggctgag ttttttctgt tgctcctgcc agtatttcaa agccatcagc gcagcgactt
      481 ccgcatttgc cttttgtttt tcctttatct tctctggtgt ataatagttt ttaacgaagt
      541 gggtatcaaa atttccgctg aagaatgcat cgtgttcaaa cacaaatgtg ccaaagggta
      601 aagtagtagc cacgccttca atcttataat ctttgattgc ctgcaacatt ttctggatgg
      661 cttccgtgcg ggttttacca tgcaccacca gtttggcaat catcggatca taataaatag
      721 gaatagtcat tccttcttca tagccatcat ccacacggat gccttctccc accggtttct
      781 ggtatttggt aagagtacca atggacggca ggaaattatt cagcgggtct tccgcatata
      841 cccgcagttc aatggcatgt ccgttaggtt tgatatcatc ctgggtaacc gataattttt
      901 ccccgcgggc aatccggatc tgttgttcca ccaggtcgag accgatgatc atttcagaaa
      961 ccgggtgttc cacctgcagg cgggtattca tttccaggaa atagaaattc agtttatcat
     1021 ccaccaggaa ttccaccgtt ccggtgctta cataattaca ggatttagcc accatcacgg
     1081 ctgcttcccc gatacgatcc cgcatttccg gagtcaccac agctgaagga gtttcttcca
     1141 ccactttctg atgacggcgc tggatactgc attcgcgttc gagaccatga attacattac
     1201 catatttatc agccaccacc tgtacttcga tatggcgggg agaggttaca tatttttcaa
     1261 taaagacgga gccatccccg aatgaggatt tggcctcact gattgctctt tccatttgct
     1321 cctgcatatc atccgcgcgt tccacgatac gcatcccctt acccccacca ccggcagagg
     1381 ctttgatcag aatcgggtaa ccgattttat tggcggtttc gatggcttca ttcacatctt
     1441 caatcgcatg atcaatgccg ggtaccatgg gaatattgta ttttttcaca ctgtccttgg
     1501 ccgccagttt ggaacccatc acccgcatgg cttccggggt ggggccgata aactcaatcc
     1561 ctgcatcggt tactttctgg gcaaaatcag cattctcact caggaaacca taccccgggt
     1621 gaatgccatc cacaccgagt tctttactga ttgcgattat tttatcgcca ttaagatatg
     1681 actgactgga aggtgctgca cccaggtgaa ccgcttcatc cgcgaataat acatgggggg
     1741 cgtgacggtc ggcatctgaa taaaccgcca ctgatttgat acccatctta cggatggtac
     1801 gcataatacg aagagcaatt tctccccggt tggccacaag tatttttttc attagaatcg
     1861 ttttaaagtt gaattattca agttccacca gaacctggcc tttatccaca gcctgaccgg
     1921 ttgtaacggc aatacgtttg atcaccgcat tgctctggat cataatactg ttttccattt
     1981 tcatggcttc caggatcagg atacggtccc cttcttttac ttcctgtcct tccttcaccg
     2041 caatctccag aacgagtccg ggcatgggtg ctttgatctc tttaatatgc ttggtggaag
     2101 cactgctgaa gcccatactg tccagcatct ggtccagcgg atctttgatt tccatatcaa
     2161 aggactcacc gtcgacttcc agttttattt tcttgccatt gatatctgct tccagcaggc
     2221 gggcattcac tgatcggtga tccatgatca ggttgaattc ctcgggtgat tttttgaaaa
     2281 agtcagcggc atcgatggct gcctggtcaa agaagaagac aaaatcattt gccttcacct
     2341 taaaggtttt agttgattct gacataatac aggggattta ttggtaagtt acctgaattt
     2401 aaaaagatgg aaagcctttt catattaaca gggcccggct gggcattagt ggaaagctta
     2461 cggctttgtg ccggtaagtg gaaaacaatc agtatgcaag gttgacattt acaggcggtt
     2521 attggatgat tatcctcatt ccattaccaa attaatatca gttcggccaa ttgtcaggga
     2581 aaagtcaaaa ggactgaaat tgaagccggc ttcccgattt ttgtcaggga aaacacagta
     2641 taccctgaca gaataaccgc atttctccac ggcacagatt tcgtgcatcc ggtaccgtat
     2701 tcattattta ttcactaaaa acaaaaaaac atgtactaca atcagtcatg tatgcgccag
     2761 cgtcaggctg cagcgcagga ggccgaaaaa ggccaacagt acggaaacag gcccgcgttt
     2821 aaccgatcta tgcaacgggc ggctgtcaac atttacaaga cagccaacag ttatgaaatg
     2881 ctggtatttg ctcccggacg tattcgtgaa cattttcacc tggatgtaaa cggtcaggag
     2941 ctcagtgttt cgtacacacc gccggaggga tttccccggc cggaatgggt gctgcgggaa
     3001 tacagccgtg gcggatttaa gagaagtttt accctggatg aaagcattga caaaaatgcg
     3061 atcacggcaa aatatgccga tggcgtttta caggtcagtc ttccgctctt agcggagaag
     3121 caatccctga aaaaggaaat tgtgatccag taagtctggt atttaacgaa cgggctgttc
     3181 tttcagcccg ttcttttttt tatcgctttg tgcatgaaag atactgtgca cattggcctt
     3241 ccattccgga aggaaatgta ccagtatgat ttcttctatt aattcaagca atgtgatacc
     3301 agtggcgatg taaaaaaaga ccacggaagg atgacctgta aaaaacgtaa ggatcaggaa
     3361 gcaaccttgc agcaacgctg ctgtcttggc cagccaggta tgaaaattcg acatccggcc
     3421 atagcggtaa aaagcataag acagctgtgc caggaatacc ccggccagta tcatcaatat
     3481 cgtaaaatgg gtcttgacaa attcagggaa aataatgatc agacccgtca gtgccactgc
     3541 cacagtcagg tcatccccga tcgaatcaag ctttgtaccg gcgatactcg tcaccttgaa
     3601 acgcctggcc agaaaaccat caatcaggtc tgtaaagaaa ctgagtgcaa caccccattt
     3661 aaatagcgga tactgcccgg taaataacaa ccacagcaat acaggaaccg ttacaatgcg
     3721 gtagagtgtg atggcgttga cgaggtaata tggtcgggga tgggaggaca agggtttgaa
     3781 cggtttagtt gtttagtcgt ttagacgttt agaggacact cccgtatttc gtacctcgta
     3841 ctttgtactt cgtacttagg agaggtgttt gcgaaaccat tccccggcta tttccgccac
     3901 ttcctgcagt ttgccgggtt cttcaaataa gtgggtggcc ccttcaatca ctacgagttc
     3961 ttttttgcag gtaagtcttt caagagcctg ttggttcagt tctgttactt catcatccag
     4021 tgaaccggtg atgagcagca cgggcgattt cacttgtggc agagcgagga gggccagatc
     4081 aggccggcct ccgcgggata cgactgcatt gactataccg ggcatcaggg aagcagctcc
     4141 caaggcagat gcagcaccgg tactggcacc aaaaaaacca atttgataat tgcggaatac
     4201 gggctgagcc tgtatccaat gtgtaacggc agcgagtctt tgtgtgagta aactgatatc
     4261 aaaacgcgcg gagttggcaa tactctccct ggaggtcagc aggtcaacga gtaaggtggc
     4321 cattccttta tcattcaata attccgccac atacttattg cggggactaa agcggctgct
     4381 gccgcttcca tgagagaaga taattatgcg gtcagcgttc tccggcactt ccagcctacc
     4441 ttccagggta actgatccca ccgggatata tacattttcg cctatttctt ttttcataat
     4501 ggggaagccg ggtaaaccgg taatggccgc ttaccacaat gaatgtacag agagaaccaa
     4561 cagtgctgca gtgagttcac tcatacgcct gaatgaatct ggtcataatg aacgggcagg
     4621 aaacagggac aggcaggtcc gttaatgtaa aataaactag ctttaaacaa agatatttat
     4681 gcccgtcagt tatttcatct acggttccgg aggcttacta gtaatcacat tgctggtcta
     4741 tcttttcagc aggaagagga aggactgatc atatcttttt tttctttggg ggaagcatca
     4801 ggtcacggat cactaccgag gcggccactg ccccgaatac agccggtaaa taggaaatag
     4861 ttccataagc cgatttttta taattttttc catcggtcag catcaggctt tctttaatag
     4921 gttcctccgg ggaaaagacc gctttaatcc ctttgcggat tccatattgt cttttcagtt
     4981 gtttacgtac ctgttgcgca aaaggacagt tatatgtttc cgatatatcc accacctgca
     5041 accgggtggg atcaagtttg gccccggcgc ccatactgct taccatggac aatccgcttt
     5101 caaatgccag ttttataaac gtgatcttgg gtgtaatgct gtctatggcg tcaatgatat
     5161 aggagggccg gtgtaacaat tgttgttcca ccatggccgg attgatgaat tcacggatga
     5221 cgtccagttc cagttcaggg ttaatggctt tgagccgctc tgccataatc agggctttcg
     5281 actccccgtg attagtagcc agtgctggta attgccggtt acggttactg ggatccacca
     5341 catcgccatc tatgatggtc atcttaccca ccccgctgcg ggcaatgaat tcggcagcat
     5401 aggaaccaac gccaccgagt cccaccacca ttacatgtga ctgagccagc cgctgaaggg
     5461 cttcttcccc gattaataag ctggtccggg ataaccagga gaaatcactt gtcataattg
     5521 aaccgcgtta ccgaaaaccg tttccgcatt tttttgaatc tgcaaaacaa gggaattgtg
     5581 atcaattgat aaagcttttg cagcctgctg ataaatttcc ccaatagaaa gttccgcatc
     5641 atccgtttcc aggaaaaacc gggttgcggg aacggaccgt aatacctcct gtataccggg
     5701 ccggtttaat gcttttccca atgaaatata gtagcccttt ccggtaatcc ggcgggccag
     5761 ttgactgttt ttattatagc cgtgaaagag taccgggacc cggatagtgg cttcttccaa
     5821 tgaagccagc acctcctccc aggcccgaac acagtgaata actaagggtt tccctatgga
     5881 atttgcccat tccacctggg cgcgaaatac ctgtaactgc agggaaaaag gggtggcaca
     5941 tactttatcc agtccgcatt ccccgatggc cagtacctga tcatgggtgc ttttctgctg
     6001 caatgaacag agctggttct cccaatcagc cgcctgtata taccaggggt gtattcccat
     6061 ggaatacagt cctggcaagg gttctggctc atccggggta ttcaggttcc ggagcaccca
     6121 ctcccctgtt cccgggggct tgtgtgaatg tatattgatg tacaaagggc aattttagta
     6181 ccacaattta gtaactccat tgggtgaaac atcccttttc attgggaaaa atgggattta
     6241 cctgtggaaa aacaatcttt aaaaatagca tggatttttt tctccctgtg cgaacgagtg
     6301 tagaaatcat ctatatttga tatcggtata acgcataccc attaacattc aaaatctaaa
     6361 aaacatggca acaaagaaaa aagcagcaaa gaaagccgct gctaaaaaac ccgtaaagaa
     6421 agccgctgct aaaaagcctg ctgctaaaaa acctgctgct aagaaagccg caaaacccgc
     6481 taagaaaaaa tctgcacgta aacccaatgc tgctttcatg aaagcactga ctccctctgc
     6541 tgccttagcc gctgtggttg gagcaaaacc attgccccgt acagaagctg ttaaaaaaat
     6601 ctgggcttac atcaaagcca acaaactgca ggacgctaaa aacagacgta atgttaatac
     6661 tgatgctaaa ctgaaagttg ttttcggtaa caaagcacag gtttctatgt ttgaaatcgc
     6721 gaaattcctc ggcaaacacc tgagttaatt tcattccac
//
LOCUS       DAOPMW010000158         6729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12939_length_6729_cov_9.339077, whole genome shotgun sequence.
ACCESSION   DAOPMW010000158 DAOPMW010000000
VERSION     DAOPMW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12939_length_6729_cov_9.339077"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLU11_08590"
     CDS             <1..411
                     /locus_tag="PLU11_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HPN59217.1"
                     /translation="KGTNPHILTLSPPVSMEPKWFEKHVAYTLTKFNMSMMSIGWAKE
                     LAKYKIAANSLWPRTTIDTAAVRNLLGGAMLANMSRTPDILADAAYAILSRPSGECTG
                     NLFIDEQVLAQEGITDLEKYSVVPGAQLYTDLFV"
     gene            complement(483..827)
                     /locus_tag="PLU11_08595"
     CDS             complement(483..827)
                     /locus_tag="PLU11_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPN59218.1"
                     /translation="MKPFIPAKVAEVLFALIIAYFGYNHFSRAGAMSGWVPDFMPGDP
                     KIWIYVVGACFILAAIAILTELQKVMACYLLAALLLLLGFLVHWKNGILSVDFLKNTS
                     MALMAILIGSKK"
     gene            complement(945..2492)
                     /gene="lysS"
                     /locus_tag="PLU11_08600"
     CDS             complement(945..2492)
                     /gene="lysS"
                     /locus_tag="PLU11_08600"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPN59219.1"
                     /translation="MNTPHLSEQEQLRREKLAELIMMGIEPYPAPLFPVNTNSASIKA
                     DFTAEKKEEPAYQDVCLAGRVMSVRDMGKANFAVIQDGHGRIQLYLKRDDICPGEDKS
                     LYDIVWKKLTDIGDIIGIRGYVFITKTGETSVHVKELTMLSKSLRPLPIVKEAEGQTF
                     DAVTDPEFKYRQRYADLIINPTVKETFLKRTKLINSIREFLNNQGALEVDTPVLQSIP
                     GGAAARPFITHHNALDIPLYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNP
                     EFTILEFYTAYKDYFWMMETTEQLLEKAALDVCGNTDVLAGEKIISFKAPFKRISIYD
                     AIREHTGIDVSRMNEDQLRSVCKQLHIQVEESMGKGKLIDELFGGKCEHHYVQPTFII
                     DYPVEMSPLTKKHRDKQGLVERFELIINGKEIANAYSELNDPIDQRERFEEQVKLMER
                     GDDEAMFIDYDFLRALEYGMPPTAGIGFGIDRLCMLLTNNASIQDVLLFPQMRPERGS
                     EGAVDSE"
     gene            complement(2598..3254)
                     /locus_tag="PLU11_08605"
     CDS             complement(2598..3254)
                     /locus_tag="PLU11_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN59220.1"
                     /translation="MKKVLFLLAAFVFCLQINGFSQKSKVGFYTGYTNANMYGKIDGA
                     RVKDDPLPGITFGLVLDAPIGKSPISFSPTLSYVQKGRVTFNENTRANNLKEWIALRY
                     AELNANFVYNTNGAKGNWFIGGGPSISFDLPSKKVSKTEELKTETNLNFGQEANSDVR
                     GIDYGANLLTGYRMKGGFFFAFNYTVGIRNLVPVQDGNDNNIRNHCWGIRLGFLINNK
                     "
     gene            3653..4357
                     /locus_tag="PLU11_08610"
     CDS             3653..4357
                     /locus_tag="PLU11_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPN59221.1"
                     /translation="MKKIVLLLLVSFTLNSCDLLSKIPTGTGTGVTEAEAGEGIKEAL
                     GQGLVKAVLQLNKEDGFFKDAFYKILLPPDAKKIENTLRDLGMGKMVDKAILQINRAA
                     EDAAGQAKPIFVDAIKNISISDAIGLVKNGDTSATHFFRVKTTEKLVAAFQPVIKASL
                     DKLDATKYYGDIVNTYNNFPTTLKKLNPDLNAFVTNRATEALFDLVAKEELNIRQNLA
                     ARTTDLLRKVFGGGIK"
     gene            4454..5173
                     /locus_tag="PLU11_08615"
     CDS             4454..5173
                     /locus_tag="PLU11_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPN59222.1"
                     /translation="MKTKIILAAFTFFLLVQQADAQLKLPKVLQKATGGAAGITENEA
                     GEGIKEALSKGVVNAVLNLNKTDGFFGSQLYKVLLPPDAVKAESTLRKMGMGAQVDKA
                     ILAINRGAEDAVGFAKPIFLEAIKGMTLTDALNILKGPKDAATSYFKQKTQQKLIEAF
                     TPSVKSSLEKTDATKYYGDIVTTYNKLPTTFKKANPDLTAYVVGKAVDALFDQVAQEE
                     SNIRSNPLARTSDILKKVFGQ"
     gene            complement(5174..6067)
                     /locus_tag="PLU11_08620"
     CDS             complement(5174..6067)
                     /locus_tag="PLU11_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59223.1"
                     /translation="MLKTVHSYLLYYLLFLLCLAGCVPKQGIREARQDLTSINQQLKQ
                     HDAGLTALEKERKKKEQLNEIDDTASSRIKKFIDKTHHQLDTLVRNNTVLIGETALEK
                     EDWDRLRKALSFSRKTSKIIGDKIEFLNELIDQSLVLRIDQDVVFGPGKYEVEPSVAN
                     ALGRIFEPAAKEIDYLVKKYPDFPLSLVITAKGYSDATQIAEGSGLYKELKERVKLQT
                     SNPGNRELNKELSVARAEAVIRLFKNYTVNRSKSGGNIRNILYLHEGKGEALPDPKIS
                     NYQVNDPRRRVVLLFWSIFPD"
     gene            complement(6137..>6729)
                     /locus_tag="PLU11_08625"
     CDS             complement(6137..>6729)
                     /locus_tag="PLU11_08625"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPN59224.1"
                     /translation="LASCEFPKGVNEFAKAGFTEIPATLVKPPMVKESLVNMECKVIE
                     VKPLGTEGGAGNLVICEVLRMHIDDSLLDENKKIDQRKINHVARLGGDWYCVVNEQNL
                     FQVEKPNTQIGIGVDALPENIRNSSLLTGNELGQLGNVHVLPDIDPVFEDDHLKQIIL
                     YYSLNPDEMEKELHTYAKKLLAAGKVKEAWQILLAG"
BASE COUNT         1777 a         1612 c         1502 g         1838 t
ORIGIN      
        1 aaaggaacca atccgcatat cctcacttta tcaccacccg taagtatgga gccgaaatgg
       61 ttcgagaaac atgtggcgta tacgctcacc aagttcaata tgagtatgat gtctattgga
      121 tgggcaaagg aactggcgaa gtacaagatc gcggccaact cgctatggcc aagaactacg
      181 attgatactg cggccgtacg gaatctgttg ggaggggcca tgcttgccaa tatgagccgc
      241 acgccggata ttctggcgga tgcggcttat gcgatcctta gtcgtccctc aggtgaatgc
      301 acaggtaatc tttttattga tgaacaggta ctggcccagg aaggcattac cgatctggaa
      361 aaatattccg tggtgccagg cgcacagctg tatacggatc ttttcgtgtg aacataaaaa
      421 aacctgcctg cctccttcgg tacactcagg atgacaggca ggttttttat ccgttatgct
      481 gtttatttct tacttccgat cagaatagcc atcagggcca tagaagtatt tttcagaaaa
      541 tccaccgaga gtattccatt cttccagtgt accaggaaac ccagcagcag cagcaacgcc
      601 gccagcaggt aacaggccat tactttttgt aattcggtga gaatggcaat agctgccagt
      661 ataaaacagg cccccaccac atagatccag atcttcggat cacccggcat aaaatcaggc
      721 acccatccgc tcatggcacc cgcccggctg aaatggttat acccgaagta ggcaatgatg
      781 agtgcaaaga ggacttcggc gactttagca ggaatgaaag gcttcatttc gttttttttc
      841 ttaaagcaac ttacggaaaa attctaagtt gaatatcggt cgatagtcaa tagtcgttag
      901 taaatacatt gcctgtgtca gctggtctta ctaacgacta acgactactc gctatcgact
      961 gctccctcgc ttcctctctc cggtctcatc tgcggaaaca acaacacatc ctggatggag
     1021 gcattattcg tgagcagcat acagagacgg tcgatcccaa aaccgatccc ggccgtaggt
     1081 ggcataccat attcgagggc ccgcaggaaa tcataatcaa taaacatcgc ttcatcatcc
     1141 ccgcgctcca tcagtttcac ctgttcttca aaacgttcgc gctgatcaat gggatcattc
     1201 agctcgctat aggcattggc aatttccttg ccgttgatga ttaattcaaa acgctccacc
     1261 agtccctgct tgtcgcggtg cttcttggtg agcggactca tttccaccgg atagtcgatg
     1321 ataaacgtcg gttgtacata gtgatgttca cacttaccac caaagagttc atcaatcagt
     1381 tttcccttgc ccatactttc ttcgacctgg atatgcagtt gtttgcatac ggagcgcaac
     1441 tggtcttcat tcatccggct tacatcaatc ccggtatgtt cacggatcgc gtcataaata
     1501 ctgatccttt tgaaaggcgc tttgaaggaa atgatttttt ccccggccag tacatcggtg
     1561 ttgccacaaa catcgagagc cgctttttcc agtaactgct cggtggtttc catcatccag
     1621 aaataatcct tgtaagcggt ataaaattcg aggatggtga attccgggtt atgggtacgg
     1681 tccattcctt cattgcggaa attgcggctg aattcataca cccagtcaaa tccacctacg
     1741 atcagtcttt tcaggtaaag ctcattggct atccgcaggt acaggggaat atccagtgca
     1801 ttgtgatgcg tgataaaggg acgggccgcc gcaccaccag ggatgctctg cagtaccggg
     1861 gtatccactt caagggcgcc ctggttattg agaaactcgc ggatagagtt gatcagtttc
     1921 gttcttttta aaaaggtctc ttttacggtg gggttaatga tcaggtccgc ataacgctgg
     1981 cggtatttga attccggatc ggtcaccgca tcaaaagttt gtccttcggc ttcttttaca
     2041 atcggtaaag gacgcagtga tttgctgagc atagtgagtt ctttcacatg caccgaggtt
     2101 tcaccggttt tggtaataaa aacatacccg cggataccaa tgatatcgcc gatatcagtc
     2161 agttttttcc agacaatatc atacaatgat ttgtcttcac ccggacagat atcatcccgt
     2221 ttcaggtaca gctgaatacg gccatgccca tcctggatca cggcaaagtt ggcttttccc
     2281 atatcccgca cactcattac acgaccggcc aggcaaacgt cctggtaagc aggttcttcc
     2341 tttttctccg ccgtgaaatc agcttttatg gaagcggaat tagtattcac gggaaacaag
     2401 ggagcaggat aaggctcaat gcccatcatg atcagttcgg ccagtttttc ccggcgcaat
     2461 tgttcctgtt cggaaagatg aggtgtattc atgtacgctt taaggtaatt tgaagccgca
     2521 aaggtaggcc ttcggaggga gggtacaaag acaaatgcct cccggagagg aggcatttgc
     2581 agtatgttga gaataactta tttattatta atcaggaaac caaggcggat accccagcaa
     2641 tggttccgga tattgttgtc attaccgtcc tgtacaggta ccaggttccg gatgcctact
     2701 gtataattga aggcaaagaa gaaaccacct ttcatacgat atccggtcag caggttggcg
     2761 ccataatcaa tgcctcttac atcagagtta gcttcctgtc cgaaattcag gttggtttct
     2821 gttttcagtt cttccgtttt ggatactttt ttagagggca gatcaaaaga gatagaagga
     2881 cctcctccga taaaccagtt gcctttcgcg ccattggtat tgtaaacaaa gttggcattc
     2941 agctctgcat aacgcagggc aatccactct ttcaggttat tcgcccgggt attttcattg
     3001 aatgtcaccc ggcctttctg cacataactt aaagtggggg aaaaactgat agggctttta
     3061 ccaattggag catccagtac taaaccgaat gtgataccag gtaaaggatc atctttcacc
     3121 cttgctccat cgattttacc gtacatattt gcattggtat atcctgtata aaaaccgact
     3181 tttgattttt gagagaagcc gttgatttgc aggcagaaaa caaatgcggc caaaagaaat
     3241 aacacttttt tcataacagt ttttttggtt cacctactcg tctcgctttt cggtttcggc
     3301 gccgtactct tttccagggg gattttccgg gttgctcccc atccatatgt gttcagcagt
     3361 cagagcccta aggtatgaat ttaccctttt gcctgctccg gcgcaaagat attttagttt
     3421 cttaaatctt tctacatcag tatcaaaaac ttcaatatcc tgttgaccgg gggtcaaata
     3481 tagaccccat tggggctttt tccgggtttg agaccctgtt tccgctataa aaaatgaaaa
     3541 tgcccctttc ccggaccggt ttttggaaag aaaaggaggc tttaccggga ttatttttag
     3601 ctttgtcagg ccatcttaat cacggataac gaatcactta aaaccaattt caatgaaaaa
     3661 aatagtcctg ctcctcctgg tttcttttac cctgaacagt tgcgacctgc tctccaaaat
     3721 tccaaccggt accggcacag gtgtcaccga ggcggaagcc ggggaaggaa ttaaagaagc
     3781 cctgggccag ggactggtta aagcggtgct acaactcaac aaagaagatg gattttttaa
     3841 ggatgctttt tacaagatcc tgcttccccc ggatgccaaa aagatcgaaa acaccctgcg
     3901 tgacctggga atgggtaaaa tggtagacaa agcaatcctc cagatcaacc gggccgctga
     3961 ggatgccgcc ggtcaggcca aacccatttt tgtggacgcc atcaaaaata tcagtatcag
     4021 tgatgcgatt ggactggtta aaaacgggga taccagcgcc acccatttct tccgcgtaaa
     4081 gaccaccgaa aaactggtgg ccgcttttca accggtgatc aaggcatccc tcgacaaact
     4141 ggatgccacg aaatactatg gagatattgt taacacctat aataattttc caaccaccct
     4201 caaaaagctg aatcctgacc tgaatgcctt tgtcactaac cgggccacag aagccttatt
     4261 tgacctggtg gccaaagaag aactgaatat ccggcaaaac ctggcggcaa gaaccacgga
     4321 tctgctccgg aaagtattcg gtgggggcat aaaatagtac acaagcaacg aatcccttaa
     4381 atattcgtca aaatacgggg cattcttttt gtacagactc ccccggatta attttactac
     4441 tcaattcaga cacatgaaaa caaaaattat acttgcagcc ttcacctttt ttcttttggt
     4501 tcaacaggct gatgcacaac tcaagttgcc taaagtgtta caaaaagcga ccggcggagc
     4561 tgccggtata accgaaaatg aagccggcga gggaatcaag gaagcgctca gcaagggggt
     4621 agtgaatgcc gtactcaacc tcaataaaac agatggtttt tttggcagcc agttatacaa
     4681 agtactgctt cccccggatg ccgtaaaagc agaaagcaca ctgcgtaaaa tgggtatggg
     4741 cgcccaggtg gataaagcaa tcctggccat taaccgtggc gcagaagatg cagtaggctt
     4801 tgccaaaccg atcttcctcg aagccattaa gggaatgacc ctgacggatg ctctgaatat
     4861 tctcaaaggt cctaaagacg cggctaccag ttatttcaaa cagaaaacgc agcagaaact
     4921 gatcgaagcc tttacgcctt ccgtaaaatc ttcgctggag aaaacggatg ccactaaata
     4981 ttacggggat attgtaacga cttataacaa acttccgaca acgtttaaaa aagcaaaccc
     5041 tgatcttact gcttatgtgg tagggaaggc ggtggatgcc ttattcgacc aggtagccca
     5101 ggaggaatca aatatccgtt ctaatccgtt ggcccgtact tcagatatcc tgaaaaaagt
     5161 attcggtcag taatcaatcg gggaaaatac tccagaataa aagcaccacg cgtctacgcg
     5221 gatcattgac ctggtaattg ctgatcttcg gatctggcag tgcttctccc ttgccttcat
     5281 gcaggtagag aatgttgcgg atattaccgc cactcttact tcggttgacc gtataatttt
     5341 taaataaacg gatcaccgct tccgcccggg ctactgataa ctctttattg agttcacgat
     5401 tacccggatt actggtttgt aactttaccc gttcctttaa ttctttatag agacccgacc
     5461 cttctgcaat ctgtgtggca tcggaataac ctttggcggt gatcaccagg gagagcggga
     5521 agtccgggta cttttttacg aggtaatcaa tttctttggc ggccggttca aaaatccgcc
     5581 caagggcatt ggccacggaa ggctccactt catatttgcc cggtccaaac acgacatcct
     5641 gatcgattcg taacaccagg ctctggtcaa tcagttcatt cagaaattct attttatctc
     5701 caatgatttt tgatgttttc cgggagaagg aaagggcttt gcgcaagcgg tcccagtctt
     5761 ctttttcaag ggcggtttcg ccgatcaaaa cggtattatt ccgcaccagg gtatcgagtt
     5821 gatgatgggt cttgtcgata aattttttaa tccggctgct ggcggtatca tcgatttcgt
     5881 tcagctgttc ttttttcttt cgttcttttt ccagggcggt aagtcctgca tcgtgttgtt
     5941 ttaattgctg attgatgcta gtgaggtcct ggcgtgcttc cctgattccc tgtttgggta
     6001 cacagcctgc caggcaaagc aggaaaagta aataatacag caggtatgag tggactgttt
     6061 tcaacataat gattcaggaa cggcagcctt aaaggtaaaa aacaaattca gtaattgcaa
     6121 tcggccatgt atgcaatcag ccggccagca ggatctgcca cgcttctttt actttacccg
     6181 cggccagtaa ttttttcgca taagtatgca gttctttttc catttcatcg gggttcaggc
     6241 tgtaatacag gatgatctgt ttcaggtgat catcttcaaa aaccggatca atgtccggta
     6301 agacatgtac attcccaagc tgaccaagct cattcccggt cagtaatgaa ctgttccgga
     6361 tattctccgg taaagcatct actccgatcc ctatttgtgt attgggcttt tccacctgga
     6421 agaggttctg ttcattgacc acacaatacc agtcgccacc cagtcttgcc acatggttta
     6481 ttttacgttg atcgattttt ttgttttcat ccagtaagga atcatcaata tgcatgcgca
     6541 acacttcgca gattacgaga ttgccggcac ctccttcagt gcccagcggt tttacttcga
     6601 tcactttaca ttccatattc accaggcttt ccttcaccat gggtggtttt accaatgtgg
     6661 caggtatttc ggtgaaaccc gctttggcaa actcattcac tcctttggga aattcgcagc
     6721 tggccagtg
//
LOCUS       DAOPMW010000159         6718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_12971_length_6718_cov_9.425034, whole genome shotgun sequence.
ACCESSION   DAOPMW010000159 DAOPMW010000000
VERSION     DAOPMW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12971_length_6718_cov_9.425034"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2829)
                     /locus_tag="PLU11_08630"
     CDS             complement(<1..2829)
                     /locus_tag="PLU11_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN59225.1"
                     /translation="MNLNKLLSRQLNKYVPAEMRDNPAMERFLQAISESYDSYERDNE
                     LSNHAFSISEQEYKQLNDKLQQESQIREISIKSLKESVKKLDENYLSREDDDLIDILY
                     YLKIQIAKRSDAENELTATANRLSSLIQNIQAGILVETENRKVVLTNAEFCSIFMPGK
                     DLEALIGTDCSSAAEKSKQLATDPEQFVERIQTIIREKKLVINEEISFIDGRFFERDY
                     IPVFINNEYKGHLWKYRNITTNKKIRDEIAESEKRHRLIMNAALDAIIHIDINGNIRF
                     WNQQAENIFGWKADEVRGKDMADLIIPEKYRDAHRNGMLHFRKTGEGPVLNKPLEITA
                     INRQGQLFPVELAIIPIRENDTVFFCGFIRDISARKEAEEKSKANEELLQFALEGAGD
                     GIWEFNFSTRKKYYSDQNKVLLGYNHDEALPDWLEQMHPEDAFIFENLEKDYLDGSIR
                     SHKKEYRILHKNGKYNWIMDRGRVVAWDETGKPLRLVGTHTDITRLKETENELRRLSL
                     VAMNSKDGIVFTKADGKIFWYNERFQQLSGYETEEILGNTPIQLLRGEASEKSVIKQM
                     LDQFYAGQGFSVEMIGYNKTRQPSWLKAKGYPVYDEKEDFTEYFTIIEDISQEKEQEN
                     RLRLLSQIAEDNINAVVTTDETSRITWVNKSFIKMTGYEMEEVVGKSPAEILHGPETN
                     KVTIHYLKKQIIKKLPFDAELINYRKDGSKYWVRIKGQPIYDEKGVMTGFFALEEDIT
                     KEKESDKQIREFESRFRKALEQVGDNAWEHNFKTGKTYFSNTSSLLHNFNIGEHEDNA
                     SVWWNLVHPEDIPLLEENNNAYLSGERDNHSLEYRLINKEGTIRWVLDRGVVIERDEQ
                     RLPLRIAGTHTDITNRKQAEQSLLVNEEKYRSIIANMNLGLLEVNMEEVIQFANNSFC
                     EMSGYSLEELIGKKAAELFVRGDNTEIAE"
     gene            complement(2819..3958)
                     /locus_tag="PLU11_08635"
     CDS             complement(2819..3958)
                     /locus_tag="PLU11_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HPN59226.1"
                     /translation="MKSAIYSYHSSRLFRHPASREINEDNAGLVLCFGAKSLLSGKPV
                     YQMLKEKFPAANIALCSTSGEIFHTEVMDGTLSITAIEFEKTRVVPQQISINAFENSY
                     EAGKALIRKFDTAGLTYIFVLSDGNHVNGSELVRGMNEMVGDQVLITGGLAGDGSNFS
                     STLVGLNNNPEEGLIAGLGFYGNNLQVLHGSQGGWETFGLEKMVTRSSSNELFEIENY
                     SALELYKQYLGPEAEGLPGSALLFPLAVKLPGSDEPVVRTILSIDDKKGSMIFAGDIP
                     VGSKVRFMKANFDKLTNAASGAAALTYDKTAAYPDLAILISCVGRKLILKSRTEEEVE
                     AVNEVFGHQTLLSGFYSYGEISPLKKGGSCALHNQTMTITTFHES"
     gene            complement(4068..4199)
                     /locus_tag="PLU11_08640"
     CDS             complement(4068..4199)
                     /locus_tag="PLU11_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59227.1"
                     /translation="MAITGKTRPANVHPVEFVIKTAALPATAGLARSIVFFNWKDYA"
     gene            4577..5170
                     /locus_tag="PLU11_08645"
     CDS             4577..5170
                     /locus_tag="PLU11_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HPN59228.1"
                     /translation="MNKNFIRLATESDAAELLNIYAPYVSGTSITFETTVPDTRSFGK
                     RISQYLDFCPWLVSESDGTITGYAYASRYRERVAYQWSVECSVYIHAAYMRHGIASAL
                     YSALFEILQQQGFRNVYAVINLPNDRSVALHERLGFRHFATYEQVGYKLGEWKNVGWW
                     QLVLNSFDEEPAAPVPFHELNKDFLEELFERSSRRLA"
     gene            5467..6675
                     /locus_tag="PLU11_08650"
     CDS             5467..6675
                     /locus_tag="PLU11_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPN59229.1"
                     /translation="MDNQQNNLPKDFFKQFKKKEDFQSFFSDLYKQGVEEMLKAELDE
                     HLGYEKHSKEGHNSGNSRNGSYSKKVKTDSLGDLVLNIPRDRNSEFEPHLIPKGSRMS
                     DKLEESIIGMYSRGMTTSDISQHVKEVYGVDVSEGTISNVTNRIIEHVRDWQNRPLES
                     VYFTVWMDGIVLKVKQNGKYINKCIYLVIGLKQDGLKEVLGMWMAETESASFWMSVLT
                     DLKARGVEDILIACTDNLKGFTEAIKAVFPQTVTQLCVVHQIRNSCKFVAWKDRKPFC
                     ADLKTIYGAVNREAAEQALEKADSVWGSKYGYAIDSWRRNWTDLTSYFDFPLEIRKII
                     YTTNTIENLNRGIRKYTKTKVQFVDDQAAQKAVYLAIMNIEKKWTMPIPNWGYILQQF
                     LIIFENRCRI"
BASE COUNT         1746 a         1439 c         1522 g         2011 t
ORIGIN      
        1 ttctgcaatt tcagtattat cgccacgcac aaaaagttca gctgcttttt tcccgatcag
       61 ttcctccagg ctatagcccg acatttcaca aaaactgtta ttggcaaact ggatcacctc
      121 ctccatattt acttcgagaa gccccaggtt catgttggca ataatgctcc ggtatttttc
      181 ttcattaacc aataaggatt gctcggcctg tttacggttg gtgatgtccg tatgggtacc
      241 ggcgatgcgt aaaggcaggc gttgttcatc ccgttcaatg accacccccc ggtcaagtac
      301 ccagcggata gtcccttcct tattaatcag ccggtattcc agggagtggt tatcacgttc
      361 cccactcagg taggcattgt tattttcttc taataaggga atatcttccg ggtgaacgag
      421 attccaccag acggaggcat tgtcctcatg ctccccgata ttaaaattat ggagtaagga
      481 agaggtattg gaaaaatagg ttttgcccgt tttgaaatta tgttcccagg cattatcacc
      541 cacctgttcc agggctttac ggaaacgact ttcaaattca cgtatctgtt tgtctgattc
      601 tttctctttg gtaatatctt cctccagcgc aaaaaatccg gtcatcaccc ctttttcatc
      661 atagataggc tgccctttga ttcgtaccca gtattttgac ccgtctttcc ggtaattgat
      721 cagttctgcg tcaaaaggca gttttttgat gatctgtttt ttaaggtaat gtatggttac
      781 tttattggtt tccggaccat ggaggatttc tgccggactt ttcccgacca cttcttccat
      841 ttcatagccg gtcattttta tgaaactctt attgacccag gttatccggc tggtttcatc
      901 cgtggtgacc acggcgttta tattatcctc ggcaatctgc gataacaggc gtaacctgtt
      961 ttcctgttct ttctcttgag aaatatcttc gataatcgtg aaatactcag taaaatcttc
     1021 tttttcatcg taaacagggt atccttttgc ctttaaccag gagggctgac gggttttatt
     1081 gtaccctatc atttcaacag agaatccctg tcctgcataa aactgatcca gcatttgttt
     1141 tatgaccgat ttttccgaag cttcccctcg caataattgt atgggcgtat ttcccagaat
     1201 ttcttctgtt tcataaccgg acaactgctg aaaccgttca ttataccaga aaatctttcc
     1261 gtctgctttg gtaaaaacaa tcccgtcctt gctgttcatc gccaccaggg aaagccggcg
     1321 cagttcattc tctgtctcct ttaaccgggt aatatccgta tgtgtaccga cgaggcgtaa
     1381 aggttttcct gtttcgtccc aggctaccac acggccccgg tccatgatcc agttatattt
     1441 gccgttttta tgtaggatcc ggtattcttt tttatgcgat cggatgctgc catccaggta
     1501 gtctttttcg agattttcaa agataaaagc atcttcgggg tgcatctgtt ccaaccagtc
     1561 aggcagcgct tcatcgtggt tatatccgag cagcacttta ttttgatctg aataatactt
     1621 tttccgggtt gaaaaattaa actcccagat gccgtcaccg gctccttcca gggcaaactg
     1681 gagcaattct tcattggctt tgcttttttc ttccgcttct ttacgggcag aaatatcccg
     1741 tataaagcca cagaagaaga ccgtgtcatt ttcacggata gggataatgg cgagttcgac
     1801 cgggaaaagt tgcccctgtc tgttgatggc cgtaatttcg agcggtttgt tcaatacagg
     1861 accttcacct gttttcctga aatgcagcat gccgtttcgg tgggcatccc tgtatttttc
     1921 aggaataatc aggtcggcca tatcttttcc ccgcacttca tctgccttcc atccgaaaat
     1981 gttttctgcc tgctggttcc agaaacggat attcccgttg atgtcaatgt gaataatggc
     2041 atccagggca gcgttcatga tcaggcggtg ccttttttca ctttcggcaa tttcatccct
     2101 gattttttta ttcgttgtaa tattcctgta tttccagaga tgacctttgt attcattatt
     2161 aataaaaacc ggaatataat cccgttcaaa gaagcggccg tctataaaac tgatttcttc
     2221 attgataacc agtttttttt cccggattat tgtttgtatc cgttcaacaa attgttccgg
     2281 gtcagtggcc agttgtttac ttttttctgc cgcactggag caatcggtac cgataagtgc
     2341 ctccaggtct tttccgggca tgaaaatact gcagaactcc gcatttgtta aaacgacctt
     2401 ccggttttcc gtttccacca ggatgcccgc ctgtatattc tgaatcagtg aagagagacg
     2461 gtttgctgtt gcggtaagct cgttctccgc atctgatctc ttggctatct ggattttaag
     2521 gtaataaagg atatcaatga ggtcgtcatc ttcccgggac agataatttt cgtccagttt
     2581 tttcactgat tccttcaggc tcttgatgga tatctcccgt atctgggatt cctgctgaag
     2641 tttatcattc agttgtttgt attcctgttc actgatacta aacgcatggt tggacagttc
     2701 attatctctt tcataggaat catatgattc acttattgcc tgcagaaagc gttccatagc
     2761 cggattatcc cgcatctccg ccggtacata cttattcagc tgacgtgaca ataatttatt
     2821 aagattcatg aaaggtggtt atagtcatag tctggttatg caatgcgcag ctgccccctt
     2881 ttttcaaagg agatatttca ccataagaat aaaaaccgga gagtaatgtc tgatggccaa
     2941 acacctcatt gacggcttcc acttcttctt ccgtccttga ttttaaaata agcttacgtc
     3001 cgacacagct gatgagtatg gccagatccg ggtaagcggc tgttttgtcg taagtaagtg
     3061 ctgcagcacc cgaagccgca ttggtcagtt tgtcaaaatt ggccttcata aaccggactt
     3121 tagaacctac aggaatatct ccggcaaaaa tcatacttcc ttttttgtca tcaatcgaca
     3181 gaatcgtgcg caccaccggt tcatcggatc ccggtagttt tactgctaag gggaacagga
     3241 gtgctgagcc gggtaagccc tcggcttcgg gaccaagata ttgtttgtaa agttcaagtg
     3301 cactgtagtt ttctatttca aacagttcat tggacgagga tcgggtaacc attttctcca
     3361 gcccaaatgt ttcccagccc ccctgtgaac catgcaggac ctgcagatta tttccataaa
     3421 aaccaaggcc ggctatcagt ccttcttcgg ggttgttatt taatcctacc agggttgaac
     3481 tgaaattgct cccatcgccg gcgagtcccc cggtgatcaa tacctgatca ccaaccattt
     3541 cattcattcc ccggacgagt tcacttccgt tgacgtggtt gccatctgat aatacaaaaa
     3601 tgtacgtaag acccgccgta tcaaacttcc ggatcagcgc ttttccagct tcatagctat
     3661 tctcaaaagc attaatactg atctgttggg gaactaccct ggttttttca aattctattg
     3721 cggtgataga taaggttcca tccatgactt ccgtatggaa tatctctccg gaagttgaac
     3781 agagggcaat attcgcagcc ggaaattttt ccttaagcat ttgatatacc ggctttccgg
     3841 aaaggagtga tttggctcca aaacagagta caagacctgc gttgtcttcg ttgatttccc
     3901 ggctggcagg atgccggaat aagcgactgg aatgatagga gtaaatggcg gacttcatat
     3961 gaaaagatta gttcgttttt gcggtacctg aatcggtttg gtttgggtgg tgagtacctg
     4021 caagctgaat gataaattta taaatggttt atatgttggg tacgaaatca ggcatagtct
     4081 ttccagttaa agaatacaat actgcgggca agaccggcag tagcaggtaa ggcagctgtt
     4141 ttaattacaa attcaaccgg atgaacattg gctgggcggg ttttccctgt aattgccatg
     4201 tgtggttttt ggttgggtat attaccaggc ttcaaggcag atacaaaaga gataagctga
     4261 tgtataattg gtagtttctg tggacaatcg tcgtgccaat ctgtttgcat agtagtcgcc
     4321 gtttggaatt tcataagttg gatttattgt aaattacttc tgacggagcg gttaaacaaa
     4381 tgtaaatgag gagactatat taaattaagg gtataccctt aatgataata aagggtttac
     4441 ttaagccagt gtggccgggt tttgtttcgg aatcttgcag atagactttt ggtgaggaga
     4501 aacctgttca tttttgcaga tccgtcaaaa aataaaacgg ttaaatttct actttcgttt
     4561 cagtaaactg gtttacatga ataagaattt tatacgactc gctacggagt cggatgcggc
     4621 ggaattattg aatatttatg caccctacgt atccggaaca tctattactt ttgagacaac
     4681 ggtaccggat acccgaagtt ttggaaaaag gattagtcaa tatcttgact tttgtccctg
     4741 gttggtaagt gaatcggatg gaacaattac cggttatgcc tatgcatcac gttaccggga
     4801 gcgggtcgct tatcaatggt ccgttgagtg ttctgtttat attcatgcgg cttatatgcg
     4861 gcatggaatt gcctcggctt tatatagcgc cttgtttgaa attcttcagc agcagggatt
     4921 ccggaatgta tatgcagtga tcaatttgcc caatgaccgt agtgtggctt tgcatgaacg
     4981 attgggattc cggcattttg ccacctatga acaagtaggg tataaattgg gggaatggaa
     5041 aaatgtgggc tggtggcagc tggtgctgaa cagctttgac gaagagccgg ctgcgcctgt
     5101 gccatttcat gaactgaata aggatttttt ggaggaattg tttgaaagaa gtagcaggag
     5161 gttagcatga gtaatgaata atgagtaatg aataatgagt aatgaataat gagtaatgaa
     5221 taatgagtaa tgagtaatgt tcgacgttaa taaaaaggat cagacactcc gattttcgaa
     5281 gtgactgatc ctttaaggta gtatttagtt ctttgtgtaa atagctttaa gtaactgtaa
     5341 tagggttatt cccccggttc aggtgtagcg aagagaaggc gaagcgaagc ggagccgaac
     5401 gaagcggaac ctgaaccggg gggcggcggc aaaatggccc cgatttttta actttaaata
     5461 cagtttatgg ataatcaaca aaacaatctc cccaaggact tcttcaagca attcaaaaag
     5521 aaggaggatt ttcaatcctt tttttcagac ctctataagc aaggggttga ggaaatgctc
     5581 aaagccgagt tggatgagca cctgggttac gagaaacaca gtaaggaagg tcacaacagt
     5641 ggcaatagtc gcaatgggtc atattccaaa aaagtaaaga ccgattcgct gggagactta
     5701 gtgctcaaca ttccgcgtga ccggaacagc gaatttgagc cccatctcat ccccaagggt
     5761 tcgcggatga gcgataagct tgaagaatcc attatcggga tgtatagccg tggaatgacg
     5821 acttcagata tcagccagca tgtcaaggaa gtctatggtg tggatgtaag cgaaggaacc
     5881 atctccaatg taaccaatcg gattattgag catgtcaggg actggcagaa ccgcccgctg
     5941 gagtcggttt actttaccgt ttggatggat ggcattgtgc tgaaggttaa gcagaacggc
     6001 aagtacatca acaagtgtat ttacctggtc atcgggctta agcaggacgg tttaaaagaa
     6061 gtgctgggaa tgtggatggc cgaaacggaa tcggcctctt tctggatgag cgtgttgacg
     6121 gatctgaagg ccaggggggt agaagatatc ctgatcgcct gtaccgataa cctgaaaggg
     6181 tttaccgaag cgatcaaagc tgtgttcccc caaacagtga cacagctttg tgttgttcat
     6241 cagatccgca atagttgcaa gtttgtggcc tggaaagatc gtaagccttt ctgtgccgat
     6301 ttaaagacga tttacggagc ggtaaaccgg gaggcggcag agcaggcact tgaaaaagct
     6361 gactcagtat gggggtccaa atacgggtat gcgatagact cctggcgcag gaactggacc
     6421 gacctgacca gttattttga cttcccgctg gagatccgca aaatcatcta tacaaccaat
     6481 actattgaaa atctgaatcg gggtatccgc aaatacacca aaaccaaggt ccagttcgta
     6541 gatgatcagg cagctcaaaa agccgtctac ctcgcaataa tgaatatcga aaaaaaatgg
     6601 actatgccca tccccaattg ggggtatata ctccagcagt tcttaattat atttgaaaac
     6661 agatgccgga tctaataaca gttactgatg gcgtttacac aaaatatttt atactctc
//
LOCUS       DAOPMW010000160         6693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13037_length_6693_cov_8.409310, whole genome shotgun sequence.
ACCESSION   DAOPMW010000160 DAOPMW010000000
VERSION     DAOPMW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13037_length_6693_cov_8.409310"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="PLU11_08655"
     CDS             complement(<1..1649)
                     /locus_tag="PLU11_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59230.1"
                     /translation="MKNSLLKKLLPHALALVLFLAVSALFCKPVLEGNVMNQHDNVGW
                     KGMAQNSFEYKEKNGHFPLWNPNLFSGMPNYQVAMEGKTILPDTVKVMSLGLPKPMNF
                     FFLACLCFYILALVMGVNPIVGMIGALAYSFSTYNPVIVAAGHDTQMLATAFMPLLMA
                     GLISIYNKKYWLGLALTTFGTYQELSVNHFQVSYYFFLIAVLVTLGYLVKWIKEKDWK
                     HIGIAGGLTVLAAVIGVAGNALVLMTTSEYSKFTMRGGKEISIEGDSVKKANTSGLDT
                     SYAFEYSLGKAETFTLLMPNAFGGGSYARFGEGSKVAEKLSDKGFAPNQAEQIAQSMQ
                     KYWGKIFTAGPAYLGVIIFILGLIGFVVIRSPLRWGLLAATLLGIFMTWGKNFAGFNV
                     FLFEHLPLYNKFRAPSFAQVIPQFTMAIMTILTLQQLIFAEKSREFFTKNMKPILYTV
                     GGLFGLLALLYIGMDYGSGDDAGILSGSVQQTGNDEVGRIIVSALKDERKSMFGGQIL
                     RAFGIVLMAALTLLLFAKGKISALVAGIVLLVISTLDITIS"
     gene            1703..2011
                     /locus_tag="PLU11_08660"
     CDS             1703..2011
                     /locus_tag="PLU11_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59231.1"
                     /translation="MRWLLFLSRLAFICGIAFLVSLGIAMAKTENNEPISSTIIMIGY
                     GMGMVLVPFTVLCYLLVAFSKKKLRQIVPLWLIIANIIFLCVLVYYIFYLNDPYYHQG
                     "
     gene            1986..2783
                     /locus_tag="PLU11_08665"
     CDS             1986..2783
                     /locus_tag="PLU11_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPN59232.1"
                     /translation="MIHTIIRDKPTRLFIILAGIFISTALIAEFIGVKIFSLEATLGI
                     KPAAIHLFGGTYSFNLTCGVLLWPVVFVMTDIINEYYGVKGVRFLSWLTIALIGFGFL
                     IVFGALQTTPNTWWVTSKKDIGIENMETAFHGIYGQGLGIIIASMTAFLVAQLLDVFV
                     FHKIKKWTGEKKIWLRATGSTIVSQLIDSFVVLIIAFYLYPKLVKGQGDPWPFDQLIA
                     ICIVNYIYKFIVALLLTPVIYAVHGWIEKYLGHERAAEMKKAAMSDE"
     gene            complement(2801..3265)
                     /locus_tag="PLU11_08670"
     CDS             complement(2801..3265)
                     /locus_tag="PLU11_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPN59233.1"
                     /translation="MNRFNIRVYGLLINELKQVLVSDELIRGMYITKFPGGGLEFGEG
                     TRDCLQREFMEEMNLKVTIGHHLYTTDYFQMSAFNPEHQIISIYYFAHAQEEITAPLR
                     SKPFDFDAAQMAVYEEKKETETFRFINWENFSDADLTLPIDKIVAGLVKKQY"
     gene            complement(3270..4208)
                     /locus_tag="PLU11_08675"
     CDS             complement(3270..4208)
                     /locus_tag="PLU11_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPN59234.1"
                     /translation="MIQYFKNNNLQTVEIDKPETGSWVNILPPLKQEEFFELSEGLDI
                     PIDFLTDSLDIDERSRFEEEDNVRLIVIKTPTENNSFNDSDAFYITIPICIILTHNQI
                     VTVNSFENPAIKKFLNTFPHRNPEKKNMMVLKVFEKIVQNFMDHLKEINQRRNLVEQK
                     LYASSENEQLLQLMRIQKSLVYFVTALRSNEMLMMKMERTNFLGLNEEEKEFLNDLIV
                     DNSQALEMANIYTNILSSTLDAFASIIANNQNQVLKRLAVITIVMTFPVLIASLFGMN
                     VPSGFEHSPYAFYIVALLSLAIAVAIGWLFLRKKIF"
     gene            complement(4263..5051)
                     /gene="dapF"
                     /locus_tag="PLU11_08680"
     CDS             complement(4263..5051)
                     /gene="dapF"
                     /locus_tag="PLU11_08680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPN59235.1"
                     /translation="MKFHFYKYQGTGNDFILADNRGQAYSGLTTAQINHLCNRRFGIG
                     ADGLMLLNTLEGYDFEMKYYNADGKEGSMCGNGGRCITKFAYHLGIHRETYHFLASDG
                     PHEAEIDIEGIVSLKMKDVDHIRKFHGDFILNTGSPHYIRLVKEVMDLDVYKKGHEIR
                     YSRDFAEEGINVNFVEQQDNTNKIKVRTYERGVEDETYSCGTGVTAAALMSYHNENGY
                     NDIEVTTLGGNLTVEFERTDNETYHNIWLCGPAERVFEGTLEIN"
     gene            complement(5051..5929)
                     /locus_tag="PLU11_08685"
     CDS             complement(5051..5929)
                     /locus_tag="PLU11_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPN59236.1"
                     /translation="MSRIAESELIINDRGAVYHLDLRPEELGDTVVTVGDPDRVAEVS
                     KYFDRVEVKRQHREFISHTGLVGKKRITVLSSGIGPDNIDIVLNELDALVNIDFVSRE
                     IKKELKSLNIVRLGTSGSLQADIPVDSFVASTHGLGVDNLLNFYRLDHNDEEQQLLHS
                     FITHTQIHSQTGHPYISSCAASLIKHFVTDFHQGITVTCPGFYGPQGRVLRLGIRNPN
                     LINRLTDFRFGQHRISNFEMETSAIYGLGKLLGHNCLALNAIVANRVKQEFSKDGKAA
                     VENLIKKFIEQFADHI"
     gene            6002..6072
                     /locus_tag="PLU11_08690"
     tRNA            6002..6072
                     /locus_tag="PLU11_08690"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6034..6036,aa:Cys,seq:gca)
     gene            6347..>6693
                     /locus_tag="PLU11_08695"
     CDS             6347..>6693
                     /locus_tag="PLU11_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59237.1"
                     /translation="MKNLAIFFTAVSLILFSAFISAQAPDLKTAANFVFFTTTGAVSN
                     TGTSFVTPRIGTNNGAITGFDLLPGQTESGNSITAEAAADLQDAYNLLKLSEQIFPTH
                     AAILGNGETLLPGT"
BASE COUNT         1732 a         1592 c         1490 g         1879 t
ORIGIN      
        1 ctggagattg taatgtccag ggtactgatc accaacagta cgattccggc caccagggca
       61 gaaatttttc ccttggcaaa taggagcagg gtcaaagcag ccatcaagac aataccaaag
      121 gcacggagga tctgtccgcc aaacatggac tttctttcat ctttcagagc agaaacaata
      181 atacggccca cttcatcatt acctgtttgt tgtacagacc cggaaaggat accggcatca
      241 tcaccgcttc cataatccat tccgatatac aacagtgcca gtaagccaaa taatccacct
      301 actgtataca ggatgggttt catgtttttg gtaaagaatt cacggctctt ttcggcaaaa
      361 attaattgct gaagggtaag tatggtcata atggccatgg tgaactgcgg gatcacctgg
      421 gcaaaggatg gagccctgaa cttattatac aagggcagat gctcaaacag gaatacattg
      481 aagcccgcaa agttcttacc ccaggtcatg aatataccca gcagggtagc cgccaataat
      541 ccccaacgta aaggagaccg gatgaccaca aagccgatca ggccgagtat aaaaataatt
      601 acacccaggt aggcaggtcc ggccgtgaat atcttacccc agtatttctg catactctgt
      661 gcaatctgct ccgcctgatt gggtgcaaat cctttgtcgg ataatttttc tgccactttg
      721 gatccttccc caaaacgggc atagctgccg ccaccaaatg cattaggcat aaggagggtg
      781 aaggtttcag ctttacctaa actatattca aacgcataac tggtgtcgag cccgctggta
      841 ttggcttttt taacggaatc cccttcaata gatatttctt tgccaccccg catggtgaac
      901 ttactgtatt cagaagtggt catcaataca agcgcattcc ctgctacacc aatgacggct
      961 gccagcacgg ttaatccgcc ggcaatacca atatgtttcc agtctttttc tttgatccat
     1021 ttaaccaggt aaccaagggt gaccagtacg gcaatcagga aaaaatagta gctcacctgg
     1081 aagtggttta cgctcagttc ctggtatgtg ccaaaagtgg tcagcgccag tcccagccag
     1141 tattttttat tgtaaatact aatcagtccg gccatcaaca agggcataaa tgctgtggcc
     1201 agcatctggg tatcgtgacc tgctgccacg attacggggt tataggttga gaacgaatag
     1261 gcgagggcac caatcatacc cacaataggg tttacgccca taacgagggc caggatataa
     1321 aaacagagac aggccaggaa gaagaagttc atgggtttgg gcagccccag ggacatgact
     1381 tttaccgtat cgggcaggat ggtctttcct tccatcgcta cctggtaatt gggcattccg
     1441 ctgaaaagat tgggattcca cagggggaaa tgaccatttt tctccttgta ttcaaaggag
     1501 ttctgggcca tccctttcca gcctacattg tcatgctggt tcatcacatt gccttccagc
     1561 accggtttgc aaaacagggc agagacagca agaaagagca caagagcaag ggcgtggggg
     1621 agtaattttt tcagcaggga attcttcata caccgtcggt atttagctgc caaaataatg
     1681 ctattttagg cgaaaattgc tgatgcgctg gcttttattt ttatcccgac tggcctttat
     1741 ctgtggcatt gctttcctgg tttctttggg catagcgatg gccaaaacag agaataatga
     1801 acccatatcc tccacaatta tcatgatcgg gtatgggatg ggaatggtgc tggttccctt
     1861 cacggtactt tgttatcttt tagtcgcttt ttcgaagaaa aaactcagac agattgttcc
     1921 gttatggctg atcattgcta atattatttt tctgtgtgta ttagtctatt atatatttta
     1981 cctcaatgat ccatactatc atcagggata aacccacccg tttattcatt attctcgcgg
     2041 ggatttttat ttccaccgcg ctgatcgccg aatttatcgg ggtgaaaatt ttttcactcg
     2101 aagcaacgct gggcattaaa ccggcggcga tccatttatt cggcggcacc tattccttca
     2161 atctcacctg cggggtcttg ttgtggccgg tggtgtttgt aatgactgat atcattaatg
     2221 aatactacgg ggtgaaaggg gtgcgtttcc tcagctggct caccattgcc ctgatcggct
     2281 ttggtttcct gatcgtcttt ggcgccttac aaactacccc caatacctgg tgggtgacca
     2341 gtaaaaagga tattgggatt gaaaatatgg aaacggcttt tcatggtatt tacgggcagg
     2401 gattgggcat cattatcgcc tccatgactg ctttcctcgt ggcccagtta ctggatgtgt
     2461 tcgtttttca taagatcaaa aaatggacgg gcgagaaaaa gatctggctc cgggccaccg
     2521 gctccaccat tgtctcccaa ctgatcgaca gttttgtggt actgatcatt gccttttatc
     2581 tctatcccaa actggtaaaa ggacaaggag acccctggcc ctttgatcag ctgatcgcca
     2641 tctgtattgt caactatatc tataagttta tcgtagcgct cttactgaca ccggtgatct
     2701 atgctgttca tggctggatc gaaaaatacc tgggacatga gagagcagct gaaatgaaaa
     2761 aagccgcgat gtctgatgag taaaaagtag tgtggtaaaa tcagtattgc ttttttacca
     2821 gtccggctac aattttatca attggcaagg tcaggtctgc gtcggagaaa ttttcccaat
     2881 ttataaaccg gaaagtttcg gtttcttttt tctcttcgta aacggccatt tgtgcagcgt
     2941 caaaatcaaa aggtttactt cgtaatggcg cagtaatttc ttcctgtgca tgggcaaaat
     3001 aataaatcga aatgatctgg tgttccgggt tgaaagcaga catctggaaa tagtcggtcg
     3061 tgtataaatg atgaccgatg gtcactttga gattcatctc ttccataaat tcccgctgca
     3121 gacagtctct cgttccttcc ccgaattcca gtcccccccc gggaaactta gttatataca
     3181 tgccccggat cagttcatca ctgaccagta cctgtttcag ttcattgatc agtaatccat
     3241 aaacccggat attaaatcgg ttcatacgat cagaatattt ttttacgtag aaacagccag
     3301 ccgatcgcca cggcaatagc gagggagagc agggcaacta tataaaaagc gtaaggggag
     3361 tgttcaaagc cactgggtac attcatcccg aacaaactcg cgatcagtac cgggaaggtc
     3421 attacaatgg tgattaccgc cagtcgtttc aatacctggt tctggttatt ggcaatgata
     3481 cttgcaaaag catccagggt actgctgagg atattcgtat agatattggc catctccagg
     3541 gcctgtgaat tatccacgat caggtcattc aggaattctt tctcttcttc attcagcccc
     3601 aggaaattgg tccgttccat tttcatcatc agcatttcat tgcttcggag tgctgttaca
     3661 aaatacacca ggcttttctg gatccgcatc agttgcagca gttgctcatt ttcactggag
     3721 gcatagagtt tctgttccac caggttccgc cgttggttaa tctctttcag gtgatccatg
     3781 aaattctgga cgatcttttc aaacactttc agcaccatca tgttcttctt ttcgggattt
     3841 cggtgcggaa acgtattcag gaatttctta atggccgggt tttcaaatga gttgaccgtt
     3901 acgatctggt tgtgggtaag gataatacag atcggaatgg tgatatagaa cgcatcactg
     3961 tcgttgaacg aattgttttc cgtcggggtt ttgattacaa tcagccggac attatcctct
     4021 tcttcaaagc ggcttctttc gtcgatatcc agggagtcgg tcaggaagtc aatgggtata
     4081 tccagtcctt ccgataattc aaaaaactct tcctgcttca ggggaggtaa aatattgacc
     4141 caggagccgg tctccggctt gtcaatttcc accgtctgca ggttgttgtt tttgaaatac
     4201 tgaatcatag cgtcgtaaag gtaagcccgc ccgggcagat tcccttttca atcttaaatg
     4261 agttagttga tttccagtgt gccttcgaat acccgttctg cagggccaca aagccagata
     4321 ttatggtaag tctcattgtc agtccgttca aactctactg tcaggttgcc gcccaatgtc
     4381 gttacttcaa tatcattgta cccgttttca ttatgataac tcatcagggc cgcagcagtt
     4441 actccggtgc cgcaggagta ggtttcgtct tccacccccc gttcataggt acgtactttg
     4501 atcttattgg tattatcctg ttgttccaca aaatttacat tgatcccttc ttccgcaaaa
     4561 tcacggctat accggatctc atgtcctttc ttatatacat ccaggtccat cacttctttt
     4621 accagcctta tataatgggg ggaaccggta ttcaggataa agtcgccatg aaatttccgg
     4681 atatgatcca catctttcat cttcagagat acgattcctt caatatcaat ctccgcttca
     4741 tggggcccat cggaggccag gaaatgatag gtctcccggt gtataccgag gtgataggca
     4801 aacttggtga tgcaccgtcc gccattgccg cacatgctgc cttctttgcc atccgcgtta
     4861 taatatttca tttcgaaatc atagccctcg agtgtattca gcaacatcag tccgtcggcc
     4921 ccgatgccga agcggcggtt gcataaatga ttaatctggg ccgtagtcag accggaatac
     4981 gcttgtcccc ggttatcagc caaaataaaa tcattacccg tgccctggta tttataaaaa
     5041 tgaaatttca tcagatatga tcggcgaatt gttcaataaa ttttttaatc aggttttcca
     5101 cggcggcttt cccgtctttg gagaattcct gttttacccg gtttgctaca atggcattca
     5161 gggcaagaca attatggcct aataatttcc ccaagccata aatagcggag gtttccattt
     5221 caaaattact gatccggtgc tgaccaaaac gaaaatcagt cagccggttg atcaggttgg
     5281 ggttgcggat acccagtcgc agtacccggc cctgcggacc atagaagccg gggcaggtaa
     5341 cggtgatacc ctggtgaaaa tccgttacaa aatgtttaat cagggaagcc gcgcagctgc
     5401 tgatatacgg atggccggtc tggctatgga tctgtgtatg agtgataaaa gaatgcagga
     5461 gctgttgctc ttcatcatta tggtccagcc ggtaaaaatt cagcagatta tccacgccca
     5521 gtccatgggt ggacgccaca aaactatcta ccggaatatc ggcttgcagg gagcccgaag
     5581 tgcccaatcg tacaatattc agtgatttca gctctttttt aatctcccgg ctgacaaagt
     5641 caatattgac cagggcgtcc agttcattca gcacgatatc gatattgtcc gggccgatgc
     5701 cggaagaaag gacggtaatc cgctttttgc ccaccagccc ggtatgactg ataaactccc
     5761 ggtgctggcg ttttacctcc acccggtcaa aatacttgct cacttcggcc acccggtccg
     5821 ggtcgcccac ggttaccacg gtatcgccca gctcttcggg ccggagatcc aggtggtaaa
     5881 cggctccgcg atcattgatg atcagctcag attcagcaat tctgctcata taactggttt
     5941 aatatgacgg aaataaatct atttcaaaaa tatgcttatt ttcgcaccgg ttttaaaaaa
     6001 gggtcctgtg gccgagtggc taggcagagc tctgcaaaag ctcctacagc ggttcgaatc
     6061 cgctcgggac ctcggaaaaa gggaacaatc gaatagattg ttcccttttt taatgcatga
     6121 aaactgccgg ttagccaggt taaggtctgt aaaagcacat atgtctggta acatgtctgt
     6181 ccatccatct ctccatgtca caagaataac agccagtaac cccgtagtat cgacacgaac
     6241 cacttagtct tttcacagtt accacttagt ctgcataaca ttatcggtag tcatcattgt
     6301 tgttggattc tatgggtaca ttgtatttta cccaaaaagc gatcctatga aaaacctggc
     6361 aatattcttt actgccgttt ctcttatttt gttctcagcc tttatttcag cacaggcgcc
     6421 tgaccttaaa acagccgcca attttgtttt ctttactacc acaggagccg tcagtaatac
     6481 cgggacttct ttcgttaccc ccagaatcgg gacgaacaat ggagccatca ccggtttcga
     6541 cttattaccc ggacaaactg aatcagggaa cagcattaca gcagaagctg ctgcagacct
     6601 gcaggatgcc tataacctgt tgaagctatc cgaacagatt tttcctaccc atgccgctat
     6661 cctgggtaat ggcgagacgc tcttaccggg aac
//
LOCUS       DAOPMW010000161         6687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13061_length_6687_cov_6.470597, whole genome shotgun sequence.
ACCESSION   DAOPMW010000161 DAOPMW010000000
VERSION     DAOPMW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_6687_cov_6.470597"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..481
                     /locus_tag="PLU11_08700"
     CDS             95..481
                     /locus_tag="PLU11_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59238.1"
                     /translation="MRQISLFILSAVFAFSATELHQLIRLPQLVAHYHEHKRTDPSMS
                     MFGFLQLHYTADHPMDNDDTDDNQLPFKTTEAIGHVDFSISIGRFHLNTQPEFETAVN
                     QISHPEGDPCHRAYAVFRPPQVNMVI"
     gene            546..1115
                     /locus_tag="PLU11_08705"
     CDS             546..1115
                     /locus_tag="PLU11_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59239.1"
                     /translation="MNAAQIHLALNHAPLFLSLIGGLLLILAIIRKNDLLTNISLYML
                     AAAALFTAPVFLTGEGTEELVEKAIGVNEMAIESHEDMAKISLIVIIITGVLALAGLL
                     LRKKPSITKALLPLLVLFSLASFGTMAQTAHLGGLIRHTELQSGTITGGKEAGNEQED
                     GDDEKKNPVINATDSTGQHQKKSKEKDDD"
     gene            complement(1142..2005)
                     /locus_tag="PLU11_08710"
     CDS             complement(1142..2005)
                     /locus_tag="PLU11_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59240.1"
                     /translation="MIAFTKPLLLAGLCLYSYLTHTQPAALQETNVAAAANAPDDLFP
                     GVQKPLPYNLPASGVVTASGADRLLFTATVKRGKLNGNWQSWYSNGTVCDSGRLVNNL
                     PDGAWVFRNEEGEIMAIRHYNADKFLRITNEMQHYHPKRSFYYLAVLYQKNKRSALHY
                     LDAAYSFPGSIFRKPVTSLQQLAESNTSPGNTYHPVFVQCVHEGLYMNYFSGGLVKDS
                     GMYKDGMKSGKWIHRDTAQAGWFQGAYKHGLPVKEWKHFDQEGRLVEVLHYDQAGRLS
                     WRKKINRQPSR"
     gene            complement(2110..2547)
                     /locus_tag="PLU11_08715"
     CDS             complement(2110..2547)
                     /locus_tag="PLU11_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59241.1"
                     /translation="MKSFYSLLFAAILFSSCSGSADSKVPELADEMCGCFDSFEKSLS
                     ADAQALMKTVATADNPQTAMMTGISKLKQEDALAFSEKLKSIGVKGSPVAACMENFDK
                     KHSKETTKDKKALMEKLLKAMRDKGNCPAGAAIVSLGLKQGVL"
     gene            complement(2734..4050)
                     /gene="rimO"
                     /locus_tag="PLU11_08720"
     CDS             complement(2734..4050)
                     /gene="rimO"
                     /locus_tag="PLU11_08720"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPN59242.1"
                     /translation="MKTKKLHRDKVNIITLGCSKNMVDSEVLSGQLLANDIDVVHENK
                     KSDHNIVVVNTCGFIDKAKEESINTILDQVELKRRGKIDKVYVTGCLSERYKNNLEAE
                     IPEVDAYFGTMELPLILKQFEADYKTELVGERLLATPQHYAYMKISEGCNRTCSFCAI
                     PLMRGQHVSKTMDDLVKEAEGLVKKGVKEVMLIAQELTYYGLDIYKKRMLGDLMNRLA
                     DVEGLEWIRLHYAYPSKFPLEILDVMRERDNICNYLDMPLQHAANNMLAAMKRQITRE
                     EMEELTIAIREKVPGICLRTTLIAGFPGETEDDVEELKGFLQRQRFDRVGIFTYSHEE
                     GTSAHDLVDDVPADVKESRAQEIMEVQQEISFELNQEKVGKVFKTLIDKKEAGRYLGR
                     TEFDSVEVDNEVVIHSAKKLPIGEFVNVKITKAYDYDLEGEVVSGE"
     gene            complement(4125..5087)
                     /gene="ftsY"
                     /locus_tag="PLU11_08725"
     CDS             complement(4125..5087)
                     /gene="ftsY"
                     /locus_tag="PLU11_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPN59243.1"
                     /translation="MGFFNKLFGKKEKESLDEGLKKTKEGFLARITKAIAGKSTVDEE
                     VLDNLEEALVGADVGVETTVKIIERIEKRVSKDKYLNAGELNKMLQEEIEEMLVNAPE
                     SNSYAFDSELPKKPYIILVVGVNGVGKTTTIGKLAYNFKKAGKEVLLGAADTFRAAAV
                     DQLTIWSERVGVPIVKQAMGSDPAAVAFDTVQSAVSRQSDVVLIDTAGRLHNKAHLME
                     ELSKIKRVIQKTIPDAPHEVLLVLDGSTGQNALEQAKHFTVTTDVTALAITKLDGTAK
                     GGVVLAIANQFKIPVKFIGVGEKMEDLLVFDKHEFVDSLFSLEK"
     gene            complement(5586..5756)
                     /locus_tag="PLU11_08730"
     CDS             complement(5586..5756)
                     /locus_tag="PLU11_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPN59244.1"
                     /translation="MAKAAKTAIKKDPKQAAEAKNYTKVIKAVRSPKSGAYTFKEQMV
                     HKDKVKDFLAQK"
     gene            complement(5836..6018)
                     /gene="rpmG"
                     /locus_tag="PLU11_08735"
     CDS             complement(5836..6018)
                     /gene="rpmG"
                     /locus_tag="PLU11_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPN59245.1"
                     /translation="MAKKGNRVQVILECTEHKTSGKPGTSRYITVKNKKNNPERLELK
                     KFNPILKKVTVHKEIK"
     gene            complement(6103..6339)
                     /gene="rpmB"
                     /locus_tag="PLU11_08740"
     CDS             complement(6103..6339)
                     /gene="rpmB"
                     /locus_tag="PLU11_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPN59246.1"
                     /translation="MARVCQVTGKTPIGGNKVSHSNIKTKRRFLPNLQKKKFYLAEED
                     KWIVLKLSTEAIRTINKNGLYSVVKQLRAKGEKI"
BASE COUNT         1703 a         1725 c         1273 g         1986 t
ORIGIN      
        1 ttattaacaa gcttttagct gaaaaacatc aacctcgggg acagggtcat ccttttattc
       61 aatgaatacg cttaagtttg gaacaaacct ttttttgcgc cagatatcac tatttatatt
      121 atccgctgtt ttcgcattca gtgcaactga attgcatcaa ctgatccggt taccacaact
      181 ggtggcgcat taccacgaac ataagcggac tgatccgtcc atgtcaatgt ttggatttct
      241 gcagcttcat tatacggcag atcatccgat ggataatgac gatacggatg acaaccagct
      301 tccatttaaa acgaccgaag caattggtca tgttgatttt tccattagta ttggcaggtt
      361 tcatctaaat acacaaccag aatttgaaac cgctgtcaat cagatatccc atcccgaagg
      421 cgacccctgc caccgggcat atgctgtatt ccgccctccc caggtaaaca tggttatata
      481 acctgttact tctgaacaaa catgttctga aaaaactatt tacttattca tttaaaatta
      541 attgtatgaa tgcagcacaa attcatctgg cattaaacca tgccccattg tttctctctt
      601 tgatcggcgg acttttactg atactggcaa tcatccgcaa aaatgatctg ttaactaaca
      661 tttcccttta tatgctggca gcagcagccc ttttcaccgc tcctgttttc ttaacgggcg
      721 aaggcacgga agaactggtg gaaaaagcca ttggcgtgaa tgaaatggcc atagaaagtc
      781 atgaagacat ggccaaaatt tcgctaatag tcattatcat aaccggtgtg cttgcgcttg
      841 caggactatt gcttcgcaaa aagccatcca ttacaaaagc cctgttgcca ttactggtcc
      901 tgttttcgct ggcttctttc ggcacgatgg cccaaacagc acacctgggc ggattgatcc
      961 ggcataccga acttcagtcc ggaactataa ccgggggaaa agaggcgggc aatgagcaag
     1021 aagatgggga tgatgaaaaa aagaatccgg tcatcaatgc aacagatagt accggtcagc
     1081 atcaaaaaaa gagtaaggaa aaagatgatg attaatcctt ttcataatac aggcggcttt
     1141 cctaccggga aggctgcctg tttatttttt tacgccagct aagacggccg gcctgatcat
     1201 aatgcaggac ttctaccagg cgaccttcct ggtcaaaatg tttccattct tttaccggca
     1261 gtccgtgttt ataggctccc tggaaccaac ctgcctgggc cgtatcccga tgaatccatt
     1321 tcccggattt catcccatcc ttatacatac cggaatcttt taccaatccg ccggaaaagt
     1381 aattcatata taatccctca tgcacacatt gtacaaatac gggatgatac gtattacccg
     1441 gacttgtatt tgattctgcc agttgttgaa gggaggtgac cggtttacgg aatatggagc
     1501 cagggaatga ataagcggca tccaggtaat gtaacgcaga acgtttattc ttctggtata
     1561 aaacggcgag gtaataaaaa ctccttttgg gatgataatg ctgcatttca ttggtaatcc
     1621 gcaaaaattt atccgcatta taatgccgga tcgccatgat ctccccttct tcattccgga
     1681 atacccaggc cccatccggc aggttattca ccaaccggcc ggaatcacat acggtcccat
     1741 tggaatacca gctttgccag ttcccgttca gcttaccccg tttaaccgta gcggtaaaca
     1801 ataaccggtc cgcacccgaa gcggttacta ccccggaagc aggcaggttg taaggcaaag
     1861 gcttttgcac acccggaaaa agatcatctg gtgcatttgc agcagcagcc acattggttt
     1921 cctgaagggc agcaggctgt gtatgcgtca aatagctgta caggcataga cctgccagca
     1981 gtagtggttt agtgaaagca atcatccctg caaaattaag cacggaaaca atacggataa
     2041 tgctctttca ataacataaa aaagcccggt aatactacca ggcttttact gcaaacaagt
     2101 ataaaaaact tatagtacac cttgcttcag tccgagactg acaatagcgg ctcctgccgg
     2161 acaattccct ttgtcgcgca ttgctttcag taatttttcc atcaatgctt ttttatcttt
     2221 tgtcgtttcc ttactatgct ttttatcaaa attttccata caggcagcta ccggtgagcc
     2281 tttgactcca atggatttta atttttcaga aaaggccagg gcatcctcct gtttgagttt
     2341 ggaaataccg gtcatcatgg ctgtctgggg attatcggca gtagcaaccg ttttcatgag
     2401 tgcttgcgca tcggcactca gggatttttc gaaactgtcg aaacatccgc acatttcatc
     2461 ggccagttcc ggtactttac tatctgcaga accggaacag gaagaaaaga gaatagcggc
     2521 gaaaagcaat gagtaaaatg acttcatttg tacaagggtt ttggtgatta ataattgaaa
     2581 ataccaccca cgatcaccct aaaccggata tattgacaag tgcaagagtt ctcttctcct
     2641 gagccaactg ctgattcata tctgtaacaa aaaagccccc aaccgaggcc tcttacttac
     2701 aacttataac ttattactta caacttttac tcactactca ccactcacca cctccccttc
     2761 caggtcataa tcgtacgcct ttgtgatctt tacattcaca aactcaccaa tagggagttt
     2821 tttagcggaa tggatcacga cttcattatc cacttccacc gaatcaaatt cggtacggcc
     2881 caggtaacgg ccggcttctt ttttatcgat cagggtttta aatactttac ccactttctc
     2941 ctggttcagt tcaaaagaga tttcctgctg tacttccata atctcctggg cgcggctttc
     3001 tttaacatcc gcaggcacat catcaacgag gtcatgcgca ctggtccctt cttcatgtga
     3061 ataggtaaag atgcccaccc ggtcaaaacg ttgccgttgc aggaatcctt tcagctcttc
     3121 cacatcatct tctgtttcgc cggggaatcc ggcaataagc gtggtacgca gacagattcc
     3181 ggggactttc tctctgatcg cgatggtcag ttcttccatt tcctcccggg taatctggcg
     3241 tttcatagcc gccagcatat tattggccgc atgctgcagg ggcatatcca ggtaattgca
     3301 aatattatca cgctcccgca taacatccag tatctccaac ggaaattttg aggggtaagc
     3361 ataatgcaac cggatccatt ccagtccttc cacatccgcc agccggttca tcagatcacc
     3421 gagcatcctt ttcttataaa tatccagtcc gtaataggtc agctcctggg caatcagcat
     3481 tacttctttt acccctttct ttaccagccc ctccgcctcc ttcaccaggt catccatggt
     3541 tttgcttaca tgctgtccgc gcatcagcgg aatagcacag aaagagcagg tacggttaca
     3601 gccttccgaa atcttcatgt acgcatagtg ctgcggggtg gccagcagcc gctctcctac
     3661 cagttcggtt ttataatcgg cctcaaactg ttttaagatc aggggaagct ccatcgtgcc
     3721 gaaataggca tccacttcag gaatttcagc ttccaggtta tttttatacc gttcgcttaa
     3781 acagcctgtc acatatacct tatcaatctt accccggcgt ttcagttcca cctggtcaag
     3841 gatggtattg atgctctctt ccttggcctt gtcgataaag ccacaggtat tcaccaccac
     3901 aatattgtga tccgacttct tgttttcatg taccacatca atatcattgg ccagcaactg
     3961 cccgctcagc acttcactgt cgaccatatt cttactacac ccaagggtga tgatgttgac
     4021 tttgtctcta tgtaatttct ttgtcttcat tttaatcaga tgtaagttgt cggttgccgg
     4081 atgccagttg tcaggactac cactcactac tcactactca ccactcactt ctccaggctg
     4141 aataaactat ccacaaattc atgtttatca aacaccagca agtcttccat tttctcgcca
     4201 acaccaataa acttgactgg tatcttaaat tgattggcga ttgccaggac aacaccccct
     4261 tttgctgtgc catccaattt tgtaatggct aacgcagtaa catcggtagt caccgtaaaa
     4321 tgcttggctt gttccagtgc gttctgacca gttgatccat ccagcaccaa cagcacttcg
     4381 tgcggcgcat ccggaatggt tttctggatc acccgtttga tcttactgag ttcttccatc
     4441 aggtgagcct tgttgtgcag gcggcctgct gtatctataa gcaccacatc cgactgcctg
     4501 gaaacagcac tctgtaccgt atcgaaagcc accgcagccg gatcacttcc catggcttgt
     4561 ttcacgatcg gcacacctac ccgctcactc caaatggtca gctgatccac tgctgctgcc
     4621 ctgaatgtat ctgccgctcc cagtaaaact tccttaccag ccttcttgaa attatatgct
     4681 aatttaccaa tggtggtggt tttacctacc ccgttcaccc caactactag tatgatatat
     4741 ggctttttag gcaattcaga atcaaaagca taactatttg actccggggc atttaccagc
     4801 atttcctcaa tctcttcctg aagcatttta ttaagctcgc cggcgttcaa atacttatcc
     4861 ttcgataccc gcttctcgat ccgctcaata atttttacgg tggtctcaac ccctacatca
     4921 gcacccacca gggcttcttc caggttatcg agcacttctt catctactgt gctcttaccg
     4981 gcaatggctt tggtgatccg ggccaggaaa ccttccttgg ttttcttcaa cccttcatcc
     5041 aggctttcct tttccttttt accgaataat ttattaaaaa aacccatggt tatattattt
     5101 aatcctgatg atacagttgt cagatgtcag ttgccagatg tcagtaaaca gcccttttta
     5161 agaaaaatta tctaaccctt ctaaacccct ctttcaacca ctatctcctc tacgacttta
     5221 taaaccctct aaacgctcct ttcaacctct tattcctttt ggccgttata aacctaacat
     5281 gtcgccccta cagggctctg agggcttttg cgaaattccc tttctataaa tagaccaccc
     5341 ctacggggcg gaatacaaag agcatctaca tccaaacagc taaaccctat ggtcaacgtc
     5401 tttctacttt caaaccgcat aaactctcct ttcaaacact ttctactata agactctcta
     5461 aactcttcgg tcagcctctt tcaactgtaa cccactctaa actctccttc agcctgccga
     5521 agcataaaca acaaaagcca ttcgtggtaa cgaatagctt ttaagatagt caggcgaacc
     5581 cgtaattact tttgggccag gaaatccttc accttgtctt tgtgcaccat ctgctctttg
     5641 aaggtatagg caccagactt cggactgcgg acagccttga tcactttagt atagttcttt
     5701 gcttctgcgg cttgtttagg gtctttcttg atcgccgttt ttgctgcttt tgccatgttg
     5761 ttaagttgat atgtttataa gttaataagt ttaccaggcc ctgtcaacca gttaacctgt
     5821 taacttttca accaattact tgatttcctt gtgaacagtt acctttttca gaatggggtt
     5881 gaattttttc aactccagac gctcaggatt gttctttttg ttttttaccg tgatataacg
     5941 gctggttccg ggcttaccac tggttttgtg ctcagtgcat tccagaatta cctgtacacg
     6001 gttgcctttc tttgccatga ttatctattt atacgttttc ttttttctta aacttaccgg
     6061 tttacttcac cggaggattg tcatcctgag cctggcgaag gattagattt tttctccttt
     6121 cgctctcagt tgcttcacta cactgtacag accattttta ttaatggtgc ggattgcctc
     6181 agtagacagt ttcagcacga tccatttatc ctcttcagcc aggtagaact tctttttctg
     6241 caggttgggc agaaaacgac gtttcgtctt gatattggag tgcgaaacct tgttgcctcc
     6301 gatgggtgtt ttacctgtaa cctgacatac tcttgccatg attgatacaa tttttgggac
     6361 ggcaaaggta gggaaaatca ggtaattact atccggattt acttcttttt ttggcaattt
     6421 tcttaacatc gggggaaaaa gccctcccgg caggttttca ggcccgaatt tacacccatt
     6481 ttccctttta ctaccggttc cccgtcctgc cccttcccgc tccccccttt aacgcccctt
     6541 tgcgaattgg ggcatttcgg ataacttcgc ctctcattat tgagtaaccc atttacttat
     6601 caactaatta cctcaaacat ggcatacgat gtagtcgttc tcggtagcgg acccggcgga
     6661 tatgtaaatg ggttactcaa taatgag
//
LOCUS       DAOPMW010000162         6621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13236_length_6621_cov_5.720835, whole genome shotgun sequence.
ACCESSION   DAOPMW010000162 DAOPMW010000000
VERSION     DAOPMW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13236_length_6621_cov_5.720835"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLU11_08745"
     CDS             complement(<1..771)
                     /locus_tag="PLU11_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59247.1"
                     /translation="MQEPPIEFQKNFFRPVCNEDEIERIFNVKNAKDYLLKLRAETIV
                     DHMRLFRVYRNKALANEKNWSLENSFISYQYINFKNKLPDELRLKCNQITYGNIFSTE
                     PNGQIFKTEYGIITTLCDSLRYFIEFMNLGLLDFKKEVPTKVQINAIRIALRVMLQTE
                     SLDFLMDPRGIIPPKIQKKMYHTYPFQMQFIAGHEYAHFLLGHLDENKITKQYLLKAI
                     FKSQSDYKPLDVYNISQKNEFEADIASINLPNYSLQEKM"
     gene            complement(1430..2248)
                     /locus_tag="PLU11_08750"
     CDS             complement(1430..2248)
                     /locus_tag="PLU11_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPN59248.1"
                     /translation="MKTVLPSLAGLFILFFLVGCSPSLNSQSKYKKLVWSEEFNYKGL
                     PDSTKWSYDKGTGCPDVCGWGNNELQYYTWNRPENARVEDGHLVIEARKEEMQGMKYT
                     SARLATKNKGDWKYGRLEIKAMLPAGRGMWPAIWMLPTKWEYGGWPKSGEIDIMENVG
                     YWPDSVLATVHTEAYNGMINTQKTKGVHQPGISTKFHIYSMEWTENEMFFYVDGKEVN
                     RFSNDKTGFAAWPFDKEFHLLLNIAVGGNWGGKFGVDDAIFPQKMLVDWVRVYQ"
     gene            complement(2366..3382)
                     /locus_tag="PLU11_08755"
     CDS             complement(2366..3382)
                     /locus_tag="PLU11_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPN59249.1"
                     /translation="MRAAILSVLFVTVLLSCGKDDPKPNNPAPTNLTVNAVVATDNSG
                     NVAFTASATNAVTYEYDYGNGVFATVPSGIVTYKYPASGTYTVNVIAKGSGGQTISRS
                     ISVTVAVTMSLIWSDEFDTPGAPDPARWGYDLGAGGWGNNEVQYYTNRLDNAVVSNGT
                     LKIILKKEAFSGSAYTSARILSRNKFSFKYGKVEARAKLPAGGGTWPAIWMLGDNIGT
                     VSWPACGEIDIMEHVGNQLNKIFGTVHHPGHSGGNADGGTVNIPNVTTEFHRYGVEWT
                     PASIKFSVDDVVFYTFTNTSSLPFNQNFFFILNVAMGGNFGGAVDPAFTMGELEIDYL
                     RVYQ"
     gene            complement(3387..4286)
                     /locus_tag="PLU11_08760"
     CDS             complement(3387..4286)
                     /locus_tag="PLU11_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN59250.1"
                     /translation="MMNTIKKLSLLCLAAVLVVSSCKKEEYAFGDIKTPKDLTLTAVI
                     AGVDVSNPNGNGTGTVTITTTATDVLTYNIDFGDGTTNVVPSGTFVKKYSTPGTNSYT
                     ITVNAIGTGGAISTISKRITVFVAFEIPAVVLNPLTGTGSKVWVTDNDAPGHFGVGPN
                     TEFAPIWYAAGPNTREACAYDDEITFSRNALNRVSMTIDNKGTSFSIGAATGVYGFSG
                     GDGCYAVNTGGTRPLAFMGATSGSTAAVSTQIQFMVPGNGIINFGTGGTTYEILSISN
                     TSLHLRNIGADGNSWYQKLKPKP"
     gene            complement(4305..6377)
                     /locus_tag="PLU11_08765"
     CDS             complement(4305..6377)
                     /locus_tag="PLU11_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN59251.1"
                     /translation="MKALKYITGLALLLAFSTGCKKDTYEDVSFVETAIAPDQLGVLF
                     EITQDNTGLVTITPNGAGAVSYDIYYGHGGTTPVKVQAGKNTTHVYPEGVYTVKIVGY
                     SVNGKTTETTKQLTVSFRAPENLEVTTTIDPSSNFKVNVTATALYETNFRVFFGDVPN
                     EVGVVFLEGQTVSHTYAAVGTYNVRVVAYSGGAATTQVTPQVTIVDPLLLPITFESPT
                     INYAFNNFDGGNATVVANPQPAGINTTAKVGKMVKGPGGQVWGGSWIGLSSPIDFSVN
                     KVFRMKVWSPRAGAKVLLKVENGANTAQFYEKEATTSVANAWEDLVFDFNAVNTANPY
                     NHIVLIFELGTVGDGTSDFTFYFDDIRQTNAIPGSLLNLPVTFDLPGINYAMVDFGGN
                     NTVTAADPDNAANNVKKTTKTNGSETWAGTTIGAGFITPLAFSATATQMTVRVYSPAA
                     GLPIRLKVEDRNDNTRSVETETLTTVANAWETLVFDFRNQATGTAALNFAYTYNMASL
                     FFNFGTAGTGNIFYWDDVTFLPVNIPGVELPLDFESGTLNYAFTNFDGGDVTIVNNPQ
                     PAGINTSTKVAKMVKNAGQPWGGSWIALAAPINFGALRTFKAKVWSPRVGAKVLLKVE
                     NMTNGAISFEKEVLTTVANGWEELTFDYTGINVANSYRKIVLIFDLGTMGDGSPNYTF
                     YFDDIKLN"
     gene            complement(6399..>6621)
                     /locus_tag="PLU11_08770"
     CDS             complement(6399..>6621)
                     /locus_tag="PLU11_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN59252.1"
                     /translation="NLHDITTTGNALYLNIIDERRLELAMEGDRFFDLVRTGQAATKI
                     TGFTAGKHEVFPIPQEEIIISGLTQNAGY"
BASE COUNT         1742 a         1600 c         1441 g         1838 t
ORIGIN      
        1 cattttttct tgaagggagt agtttggcaa gttaattgag gcaatatcag cttcgaattc
       61 atttttctgt gatatgttat aaacatctaa aggcttatag tcactctgcg atttaaaaat
      121 tgcctttaag agatactgct ttgtgatttt attctcatcc agatgtccta ataaaaaatg
      181 tgcatattca tgtccagcaa taaactgcat ttgaaatgga tatgtatgat acattttttt
      241 ttgaattttg ggaggtataa tgcctcttgg gtccattaag aaatctaaag attcggtttg
      301 cagcattact cgtagagcaa ttcgaattgc attaatctga acttttgtcg gcacttcctt
      361 cttaaaatcc aaaagcccaa gattcataaa ttcgataaaa tatctcaacg agtcgcacaa
      421 tgtcgttata atgccatatt cagttttaaa aatttgacca ttaggttcag ttgagaagat
      481 atttccataa gtaatttgat tgcactttaa tctgagttcg tcaggtagtt tatttttgaa
      541 attaatatat tgataactga taaacgaatt ttcaagagac cagttctttt cattagctag
      601 agctttattt ctgtaaactc taaatagacg catgtggtct acaatagttt cggctcgcaa
      661 ttttagtaga taatctttag catttttaac attaaaaatt ctttctattt catcttcgtt
      721 acaaacaggt ctaaaaaaat ttttttgaaa ttctattggg ggttcttgca tgatagttac
      781 gggttttatc tattggcaca caaataataa aaaacgaacc tttgaagctc tattaatttt
      841 ttattcttta taagttgcac agttttaatt gttgcttaaa atttcttttt tcaagttgca
      901 gtttccttaa tacacacggc caatctctct caatcccaat attcaaaaca ataccccccc
      961 cacacacttc gcccacctca gtcccatcaa ctactctata accccttatg aaaacttgct
     1021 gaaaccgact tcaggacccg aagccaggac tgcccgtaac cggcagcaac agacgaattt
     1081 tcaggagagg ttaaactaaa gctaagaaaa aaaaggaaat gtcccgcggg tatacatcgt
     1141 tgttgacccg gattaatcaa tcacccgtgg gaattcaggc aataaaaaac aggggccgat
     1201 tcggactccc tgttaataat tgattattag taaattatgt atagctgctg tacgctttcc
     1261 ataggtttgc tccgggactc gtccggacct tgttcggggt taaagcgcat ggagcgcttt
     1321 atctgtaggg aagtcccgga caaatataaa cccaggtctt aagtttgtcc ccattcacca
     1381 ttccccattc accattcacc attcaccatt caccattccc cattccacat cactgataca
     1441 ccctcaccca gtccaccagc atcttttgcg ggaagatcgc atcgtccacg ccgaacttgc
     1501 cgccccagtt accaccgacg gctatattca gcagcaggtg aaactcctta tcaaagggcc
     1561 aggccgcaaa gccggtcttg tcattgctga accggttgac ttcctttccg tctacataaa
     1621 agaacatttc gttctcggtc cactccatgg agtagatatg aaacttcgtt gagatgccgg
     1681 gctgatgtac tcctttggtc ttttgggtat tgatcatgcc attgtacgct tccgtatgta
     1741 cggtagcgag caccgaatcg ggccagtaac ccacattctc catgatatct atttcgccgc
     1801 tttttggcca gccgccatat tcccacttgg tgggtagcat ccagatggca ggccacatac
     1861 cgcggccggc gggcaacatg gctttgattt ctaaacgacc gtacttccag tcgcctttgt
     1921 tcttggtagc caggcgggcc gaggtatact tcatgccctg catctcttcc ttacgggctt
     1981 caatcaccag gtgaccgtct tccacccggg cattctcggg gcggttccag gtgtaatatt
     2041 gtaattcatt attgccccag ccgcatacat cgggacaacc ggtcccctta tcatagctcc
     2101 atttggtgct gtcgggaaga cccttgtagt tgaactcctc agaccagact aattttttat
     2161 acttggactg gctgttcagg gaaggactgc aacccactaa aaaaaataga ataaacaaac
     2221 cagcaaggga tggcaaaacc gttttcatga ttgatcgttt gtagatgaaa ataaaagtgg
     2281 aagtaatatc ctggaaagga cttaacgttc cactttcaag actgctcgtt aataattgcc
     2341 tgtgaaccta aaaatcgaaa ctcttttatt gataaacacg taagtaatct atttccaatt
     2401 cacccatagt aaatgcggga tccacggctc caccaaaatt gccgcccatg gccacattca
     2461 gaataaagaa aaaattctga ttgaagggca gtgaactcgt attggtaaac gtatagaaca
     2521 ccacatcatc caccgagaac ttaatcgaag ccggggtcca ttccacacca taccggtgaa
     2581 actccgtggt cacattgggt atattgaccg ttccgccgtc tgcattacca cctgaatgtc
     2641 cggggtgatg caccgtaccg aagatcttat tcagctgatt ccccacatgc tccatgatat
     2701 cgatctctcc acaggcgggc cagcttacgg ttccgatatt atcacccagc atccagatgg
     2761 caggccaggt gcctccgccc gcaggcagtt tggcacgggc ttccacttta ccatatttga
     2821 atgagaactt attcctggat aatatccggg cagacgtgta agcactgccg ctgaatgctt
     2881 ctttcttcaa tatgatcttc agcgtaccat tggatactac cgcattgtcc agcctgtttg
     2941 tataatattg cacctcatta ttgccccatc cgccggcacc gaggtcatag ccccaccttg
     3001 ccgggtcagg cgcaccgggt gtatcaaact catcggacca gatcagggac atcgtcactg
     3061 ctactgtaac cgatatagac ctggaaatgg tttgtccgcc actgcctttg gcaattacat
     3121 tcaccgtata ggtaccggag gccggatact tataggtaac gattcccgaa ggcacggtgg
     3181 caaaaacccc gtttccataa tcatactcat aggtcaccgc attggtggcc gaagcagtga
     3241 acgccacatt accactgtta tccgtagcca ccaccgcatt caccgtgagg ttagtgggtg
     3301 ccgggttatt gggcttggga tcatccttgc cgcaggataa cagtacggtt acaaacagga
     3361 ccgataatat agctgcacgc atggtattat ggtttaggct ttaatttctg ataccaggaa
     3421 ttaccatcag caccgatatt ccgcaggtgc aggctggtat tgctgatcga caggatttcg
     3481 tacgtagtac caccggtacc aaagttgatg ataccattac caggtaccat gaactggatc
     3541 tgggtggata ccgctgcggt agaaccggat gtggcaccca taaacgccag gggtcttgta
     3601 ccaccggtat ttactgcata acatccatca ccaccactga atccataaac accagtggcc
     3661 gctccgatag agaaagaagt gcctttgttg tcaatggtca tggatacccg gttcagggca
     3721 ttgcgggaaa acgtgatctc atcatcatac gcacaggctt cgcgggtatt gggcccggct
     3781 gcataccaga tcggtgcgaa ttctgtattc ggacctacgc cgaaatggcc gggggcatca
     3841 ttatccgtta cccatacttt ggaaccagta ccggtgagcg gattcaatac caccgcagga
     3901 atttcaaagg caacaaacac ggtgatgcgt ttgctgatgg tggaaatagc accgccggtt
     3961 ccgatagcgt taaccgtaat ggtataactg ttggtgccgg gcgtgctgta ttttttcacg
     4021 aatgtaccgg aaggcaccac attggtggtt ccgtcgccaa agtcgatatt atacgtcagc
     4081 acatctgttg cggtagtggt aatggtgacc gtaccggtac cattcccgtt ggggttggac
     4141 acatccaccc cggcgatcac tgccgtaagg gtcaggtctt tcggtgtttt gatgtcaccg
     4201 aaagcatatt cttccttttt acagctgctt accaccagta cggcggccag gcagagcagg
     4261 gacagttttt ttattgtatt catcatgact gaatgatttt aggtttagtt aagcttaatg
     4321 tcatcaaagt agaaggtata gttgggtgaa ccatcgccca ttgtaccgag atcaaatatc
     4381 aatacaattt tccgatagga gttggcgaca ttgataccgg tgtagtcaaa cgtcagctct
     4441 tcccatccat tggcaaccgt ggtcaatact tctttttcaa aactgatggc tccattggtc
     4501 atgttctcta ctttcagtaa cactttagca ccaacccgcg gagaccatac tttcgctttg
     4561 aatgtacgca gtgcaccaaa gttgatcggt gccgctaagg caatccagga tccgccccag
     4621 ggctgaccgg catttttcac catcttcgcc actttcgtac tggtattaat accggcaggc
     4681 tgcggattgt ttacaatggt tacatctccg ccatcaaaat tggtaaaagc gtagttcagt
     4741 gtacctgact caaaatccag cgggagttcg acacccggaa tgttaaccgg caggaacgtc
     4801 acatcatccc aataaaaaat atttccggtg cctgctgttc caaaattaaa gaagagcgaa
     4861 gccatgttgt aggtatacgc aaagttcagg gctgctgttc cggtagcctg atttctgaaa
     4921 tcaaatacca gtgtttccca ggcattcgcc accgtcgtca aggtctccgt ttcaacagaa
     4981 cgggtattgt cattcctgtc ctctactttc aaacggatcg gaagacccgc tgcaggagaa
     5041 taaacacgca cggtcatttg cgtagcggta gctgaaaaag ccagcggcgt aataaagcct
     5101 gcaccgatcg ttgttccggc ccatgtttca gacccattgg ttttagtggt cttttttaca
     5161 ttgtttgctg cattatcagg atcagcggcc gttactgtat tgttaccacc aaaatccacc
     5221 atcgcatagt tgatacctgg cagatcgaat gtaaccggca ggttcagcaa tgaaccgggg
     5281 atcgcattgg tttggcggat atcatcaaaa tagaacgtga aatcggatgt accatcacct
     5341 actgtaccta attcaaaaat cagtacgatg tggttgtatg ggttcgctgt attgaccgcg
     5401 ttgaaatcaa acaccaggtc ttcccaggca ttcgccactg aagtcgtggc ttccttttca
     5461 taaaactgtg cagtattcgc tccattctcc actttcagta acactttggc accggcgcgg
     5521 ggtgaccata ctttcatccg gaatactttg ttgacagaaa agtcaatcgg agaacttaat
     5581 ccgatccatg aaccacccca aacctgacca ccaggacctt ttaccatttt acccacttta
     5641 gccgtggtat tgataccggc cggctgaggg ttggctacca ccgttgcatt accaccgtca
     5701 aaattattga aggcgtaatt gatggtgggc gattcgaagg tgattggtaa taataaagga
     5761 tcaacaatcg taacctgcgg ggttacctgg gtggtggcag caccaccact ataagctacc
     5821 acacggacgt tataggtacc aacagcggca taggtatgac ttacggtttg tccttcgagg
     5881 aaaaccacgc ctacttcatt cggcacatcg ccgaagaata cacggaaatt ggtttcatat
     5941 aaagccgtag ccgttacatt cactttgaaa ttactggaag gatcaatagt ggtggttact
     6001 tcgagattct caggcgcccg gaaggatacc gtgagctgct tggtggtctc cgtagttttg
     6061 ccattcacac tatagccaac aatctttacc gtatacacac cctcgggata aacatgggtg
     6121 gtgtttttac cggcctgtac ttttaccggc gtggtaccgc cgtggccata ataaatatca
     6181 tagctgaccg caccggcccc gttgggggta atggttacca gcccggtatt atcctgggtg
     6241 atttcaaata agacacccag ctgatcggga gcaatagctg tttccacaaa agaaacatct
     6301 tcgtaggtat cctttttaca gcccgtggag aaggccagta ataaggccag tccggttata
     6361 tattttaatg ctttcatttg ctttcgtttt gagtttgatt aataaccggc attctgggtc
     6421 agtccggaaa taattatttc ttcctgtgga atcgggaata cttcgtgttt accagcggtg
     6481 aaacccgtga tcttggtggc agcctgaccg gtacgaacca ggtcaaagaa acggtcacct
     6541 tccatagcga gttccaggcg gcgctcatcg atgatattca ggtaaagcgc attaccggtg
     6601 gtagtgatat catgcaggtt g
//
LOCUS       DAOPMW010000163         6615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13255_length_6615_cov_7.621341, whole genome shotgun sequence.
ACCESSION   DAOPMW010000163 DAOPMW010000000
VERSION     DAOPMW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13255_length_6615_cov_7.621341"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLU11_08775"
     CDS             <1..299
                     /locus_tag="PLU11_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59253.1"
                     /translation="LQVEQTAAIAVYRIVQELLNNTMKHAAAKTAIVQLSRTDAGINI
                     TVEDDGKGFDPVILSGGKGIGWSNIQSRVEYLKGKMDVRSTPGNGTSVNIEFNL"
     gene            296..916
                     /locus_tag="PLU11_08780"
     CDS             296..916
                     /locus_tag="PLU11_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59254.1"
                     /translation="MMATKVFIVDDHYMVIEGIRSLLQHEKGMEWSGHAQTAASCLAY
                     LQREQPDVILMDINLPDMSGIDLCKEVKTKYPSVFIIGLSTFNQQSFIQKMMENGASG
                     YVLKNATQEELTEAIAMVCRGKIYLSEEASYALREQPSVTIILTRREKEVLELIAEGM
                     TNNEIAVKLFISVTTVDTHRKNLLSKFEAKNVASLIRAAIKLQLIK"
     gene            complement(961..2214)
                     /locus_tag="PLU11_08785"
     CDS             complement(961..2214)
                     /locus_tag="PLU11_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPN59255.1"
                     /translation="MKKVFTVLFLLSTLTSRAQVPVQKIIAGAERIAEYLPLLKGHSV
                     GIFANQTSMVGQTHLVDTLRKLGINIKVIFGPEHGFRGTADAGEKVGNYTDPQTGIPV
                     ISLYGGKRKPTAEDVKEVDLLLFDIQDVGVRFYTFISSLQDFMEAAFELGKPLIILDR
                     PNPNGHYVDGPVLDKTYKSFVGMQPVPVVYGMTIAEYAMMIAGEKWLSEKANARHDYY
                     RTAQNSVDTPFHFQVIKCANYTHNSVYQLPVRPSPNLPDIQSIYLYPSTCFFEGTVLS
                     EGRGTSRPFQVFGHPKLPAHLYSFTPQPNEGAKKSKWYGEICYGWDLGGSTEEVRAKT
                     GNRIQLQWLMEAYRLFPEKDKFFIVPKSGKMDQSFFNKLAGNNDLWQQIKEGKTEEEI
                     RKSWEPALNQFKNIRKKYLLYPDFD"
     gene            2340..2795
                     /locus_tag="PLU11_08790"
     CDS             2340..2795
                     /locus_tag="PLU11_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN59256.1"
                     /translation="MSQVKSGDKIKVHYHGKLANGETFDSSAGREPLAFEVGSGMVIK
                     GFDDGVTGMTVGEKKTIQIPFMEAYGPRNPEMVIEMPKDRFPAEMEVEMGMPLVMSDG
                     QGQQFQVTIVEIKEASVMLDANHPLAGQDLTFDLELVEIEGGSPLIIMP"
     gene            complement(2896..3450)
                     /locus_tag="PLU11_08795"
     CDS             complement(2896..3450)
                     /locus_tag="PLU11_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59257.1"
                     /translation="MKKWLIGSFVGAILIFVWQFLSWTVFQLHEAEAKYTPAQDSILQ
                     YLSSQFKETGTYMLPTVPPGSSQEAAEELGKTMDGKPWATLTYRTSYTHNMTRPMIRG
                     FLVGLVLVFCLIYIVTRGGTPAGVLIFAASIAMGLVTFLWGAYNEHVWFQTPVADLYG
                     PFIDSLVAWGLTGIWLGWWLNKGK"
     gene            3557..4804
                     /gene="pepT"
                     /locus_tag="PLU11_08800"
     CDS             3557..4804
                     /gene="pepT"
                     /locus_tag="PLU11_08800"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPN59258.1"
                     /translation="MFQEYRFTAAERFMRYVQVDTQSDPQSSASPTTEKQKDLGRLLA
                     AELREMGIEDAHMDNYGYVYATIPATTEKKVPVICFCAHVDTAPDCSGTGVKPILHRD
                     YQGQDIVLPDDPAQIIRMSEYPYLRKLTGHDIITASGTTLLGSDDKAGVAEIMDLANF
                     LINNKQVKHGTIKILFTPDEEVGKGTDKVDLQKLGADFGYTLDGGEAGSLEDETFSAD
                     GVQVVIHGVIAHPGYAKDKMINAMKIAGEILAALPKDRLSPESTEGKRGFIHPVRVDG
                     IAEKCTIDFIIRDFETEGLKKKEDYLRTQIEERMRTYPKASFEFNVTEQYRNMKEVLD
                     NHPHVVDFAKEAIARAGLDLKMESIRGGTDGSRLSFMGLPCPNLFAGEQAIHSKLEFI
                     SVQDMNKAVETLVYLVQVCEEKS"
     gene            4853..5794
                     /locus_tag="PLU11_08805"
     CDS             4853..5794
                     /locus_tag="PLU11_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPN59259.1"
                     /translation="MDAANWISHYWWILVLAFIVFSGLFTISQGYVGVITRLGRYTRV
                     ARPGLNFKIPLLESVARKVSIQNRSVELEFQAVTADQANVYFKSMLLYSVQNAEEETI
                     KKVAFKFFADKDLMQALIRTIEGNIRSFVATKKQAEILALRKEIVEYVKTEIDLQLET
                     WGYHLQDLQINDITFDKLILDSMSRVVASNNLKAAAENEGQALLITKTKAAEAEGNAI
                     KISAEAEKEAARLRGQGVALFRQEVAKGMTEAAEQMKQANLDTNVILFSMWTEAIKNF
                     AEYGKGNVIFLDGSPDGMEGTMKQIQALMVKQAGVGK"
     gene            6203..>6615
                     /locus_tag="PLU11_08810"
     CDS             6203..>6615
                     /locus_tag="PLU11_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPN59260.1"
                     /translation="MIDLLQITDSLTKATQSLSGDANHDGQLSFFELLQMGGWIMIPL
                     GAMMLLGIYIFAERYIAIRNAAKIDANFMNIIRDNIISGNVTAARNFAKNTNNPVARI
                     IDKGIQRIGKPIDGIEKSMANVGQLEMYKLEKNLD"
BASE COUNT         1902 a         1400 c         1621 g         1692 t
ORIGIN      
        1 tgttacaggt tgaacagaca gccgctattg ctgtttaccg gatagtacag gaattgctga
       61 ataataccat gaaacatgca gcggctaaaa cggccattgt acaactgagc cggaccgatg
      121 ccggaattaa tattacagta gaagatgacg gaaaaggatt tgatccggta attttgagcg
      181 gggggaaagg aataggctgg agtaatattc aaagtcgtgt ggaatacctc aaaggcaaga
      241 tggatgtcag gtctactccg ggcaacggga cttcggtaaa cattgaattt aatttatgat
      301 ggcaacaaaa gtttttatag tagatgatca ttatatggtt atcgagggca tccgttccct
      361 gctgcagcat gaaaaaggga tggaatggtc cgggcatgcg caaacagcgg cttcctgcct
      421 ggcctacctg caacgggaac aaccggatgt gatcctgatg gatatcaacc tgccggatat
      481 gagtggaata gatctgtgca aggaggtgaa aacaaaatat ccttcggttt tcattattgg
      541 actgagcaca tttaaccaac agagctttat tcagaaaatg atggagaacg gggcttccgg
      601 gtatgtattg aagaatgcca cccaggaaga gctgaccgaa gcaatcgcaa tggtttgccg
      661 gggtaaaatt tatttaagtg aggaggccag ttatgcactg agggaacaac cttcagtaac
      721 gatcattctt acccggcgcg aaaaggaagt actcgagctg atagcagaag ggatgaccaa
      781 taatgagatt gccgtaaaac tgtttatcag tgtgacaacc gtcgatacac accgtaaaaa
      841 cctgctatcc aagtttgaag ccaaaaacgt agcttccctt atcagggccg ccataaaatt
      901 gcaactgata aaataaccgg taggactata attgatccgc gtaaggaatg cgcttctcct
      961 ttaatcaaaa tcaggataaa gaagatattt tttccggata ttcttgaatt gattcagggc
     1021 tggctcccag cttttgcgaa tttcttcttc tgttttacct tccttaatct gctgccagag
     1081 atcattattg cctgccagtt tattgaaaaa agactgatcc attttgcccg atttcgggac
     1141 aataaagaac ttgtcttttt ccgggaaaag ccggtaggct tccattaacc attgcagttg
     1201 aatccggttg ccggtttttg ctctcacttc ctccgtgcta ccgcctaaat cccacccata
     1261 acagatttcg ccataccatt ttgatttttt ggccccttca tttggctgcg gggtaaacga
     1321 atacagatgt gccggaagtt tcggatggcc gaaaacctga aaaggccggc tggtccctct
     1381 tccttcactc agtacagttc cttcaaagaa acaggtagag gggtacaggt aaatggattg
     1441 aatatcgggc aggttaggag agggtcttac cggtaattga tagacgctgt tatgggtata
     1501 attggcgcat ttgatcacct ggaaatgaaa aggagtatcc actgaattct gtgcggtccg
     1561 gtaataatca tgacgggcat tggccttttc actcagccat ttttcgcccg caatcatcat
     1621 ggcgtattcg gcaatggtca tcccatacac gaccggtacc ggttgcattc ctacaaatga
     1681 cttatatgtt ttatccagta ctggtccgtc cacataatgt ccattggggt taggccggtc
     1741 caggattatc aggggtttgc ccagttcaaa tgccgcttcc ataaaatcct ggagggaaga
     1801 aataaaagta taaaaccgaa ccccaacgtc ctgtatatcg aacaggagca ggtcaacctc
     1861 tttcacatct tcagcggtgg gttttcgttt gccgccatag agggaaatga ccggtatacc
     1921 ggtttgcgga tctgtataat tgcccacttt ttcaccggcg tctgccgtac cccgaaatcc
     1981 atgctcgggc ccgaaaatca cttttatatt tatacccagt tttctcaggg tatccacgag
     2041 atgggtttgg cccaccatag aggtctgatt ggcaaaaatg cccactgaat gtcctttaag
     2101 caaagggaga tattcagcaa ttctttctgc accggcgatt atcttttgta ccggaacctg
     2161 tgcacgggaa gtcagggtgg acaggagaaa taaaacggta aatactttct tcatattgat
     2221 ggctttaaat cattggtaaa atacggcaaa tcaggataca gacggggggg taacttaaaa
     2281 tggatgcatt tgacatttta acctaccttg cagcctttat tttaatcaaa attaatatta
     2341 tgtctcaagt taaaagcggt gataaaatca aggtacatta tcatggtaaa ttagccaacg
     2401 gtgaaacatt tgattcttct gccggccgcg agcccctggc attcgaagtt ggaagcggaa
     2461 tggtaatcaa gggttttgat gatggcgtga ccggtatgac agttggggag aaaaagacga
     2521 tccagatccc tttcatggaa gcctacggcc cccgcaatcc ggagatggtg atcgaaatgc
     2581 ccaaagaccg tttcccagcc gaaatggaag ttgaaatggg aatgcccctg gtgatgagcg
     2641 atggtcaggg acagcagttc caggtgacca ttgtggaaat taaagaggct tccgtcatgc
     2701 tggatgccaa tcatcccctg gccgggcagg atctgacttt tgacctcgaa ctggtggaga
     2761 ttgaaggcgg aagtccgctg attattatgc cataagtaat tcggcaaagt atattgaaaa
     2821 aaagatcaac cggacaggtt gatctttttt tatatagtta cgagattcga gcccccattt
     2881 cctaattccc actccctact tccccttatt caaccaccag cccagccaga tacccgttag
     2941 tccccaggct accagagagt cgatgaacgg gccatataaa tctgcaaccg gtgtttgaaa
     3001 ccagacatgt tcattgtaag caccccataa gaaggtgacc aggcccattg caattgaagc
     3061 ggcgaatatc agaacacctg caggtgtacc acctctggtc acgatataaa tcaggcaaaa
     3121 aacaagtacc aggcccacca ggaacccacg gatcatgggg cgggtcatat tatgggtata
     3181 actggtacga taagtcaggg tagcccaggg ctttccatcc attgttttac ccaattcttc
     3241 tgccgcttcc tgagatgaac caggcggaac tgtgggcagc atataggtgc cggtttcctt
     3301 gaactggctg ctgagatact gcagaataga atcctgggcc ggagtgtatt ttgcctcggc
     3361 ttcgtgcaac tgaaatactg tccaggataa aaattgccag acaaaaatga ggatagctcc
     3421 gacaaaggag ccaatgagcc attttttcat gttggttggt tttgttgagg tagaaaagtt
     3481 aaccattttc tttgtttttg tcaaccttca gcggctttct ctttcgtcgt atcttcgtta
     3541 ttcattctaa ataactatgt ttcaagaata cagattcact gcggcagaac ggtttatgcg
     3601 ttatgttcag gttgatacac aaagcgatcc tcagtcatcc gcttctccaa ctaccgaaaa
     3661 gcaaaaagac ctgggccggt tactggcggc cgaattaagg gaaatgggta ttgaagatgc
     3721 gcatatggat aactatgggt atgtatatgc gaccattccc gctactacgg aaaaaaaggt
     3781 acccgttatc tgtttttgtg cccatgtgga caccgctccg gattgcagtg gcacgggggt
     3841 taaaccgata cttcaccggg attaccaggg acaggatatt gtacttcccg acgatcctgc
     3901 acagatcata aggatgagtg aatatcctta cctgcgaaaa ctgacagggc atgatattat
     3961 aactgcttcc gggactactt tactcgggtc ggatgataaa gctggtgtgg ctgagattat
     4021 ggacctggcc aattttttaa taaacaataa acaagtaaag cacggaacaa taaaaatact
     4081 gttcaccccc gatgaggaag taggaaaggg tacagacaaa gtggacctgc aaaaactggg
     4141 ggctgatttc ggctatacac tggatggtgg agaagccgga tcactggagg atgaaacatt
     4201 tagtgcggat ggtgtacagg tagtcatcca tggcgtaatt gcccatccgg gttatgccaa
     4261 ggataaaatg attaatgcga tgaagatcgc cggagagata ttggcagcac tgccaaaaga
     4321 ccggctttcc ccggaatcca ctgaagggaa aagaggattt atacacccgg tgagggtaga
     4381 tggaattgcg gagaaatgca cgattgattt tatcatacgc gattttgaaa cagaggggtt
     4441 gaaaaagaaa gaggattacc tgcggacgca gattgaggaa cggatgcgga cttatcccaa
     4501 agccagcttc gaattcaatg tgaccgaaca gtaccgcaat atgaaagaag ttttggataa
     4561 ccatccgcat gtggttgatt ttgccaaaga agccattgcc cgcgccggac ttgacctgaa
     4621 gatggaaagt atacgtgggg gcaccgatgg cagccggctg tcttttatgg gactgccatg
     4681 tcccaatctc tttgccgggg aacaagccat ccattccaag ctggaattta tcagtgtgca
     4741 ggatatgaat aaagcagtgg aaacactggt ttacctggtg caggtatgtg aggaaaaaag
     4801 ttgaccccgt accccttaac ttatcgtaaa ttacatttct aaaattaaaa ttatggacgc
     4861 tgcaaattgg atttctcatt attggtggat attagtattg gcctttatcg ttttcagcgg
     4921 gctgtttaca atcagccagg ggtatgtcgg tgtaataacc cggctgggca gatacacccg
     4981 ggtggcaaga cccggattga attttaagat cccgttgctc gaatcagtag cccggaaagt
     5041 atcgattcag aatcgttcgg tggaattgga gttccaggca gtaacggctg atcaggccaa
     5101 tgtgtatttc aagagtatgt tgctgtattc agttcagaat gcagaagaag aaactataaa
     5161 aaaagtggct tttaaattct tcgctgataa ggatctgatg caggccttga taagaaccat
     5221 tgaaggtaat atccggtctt ttgttgccac caaaaagcag gcagaaattc tagctcttcg
     5281 gaaagaaatt gttgaatatg taaaaaccga aatagacctg caattagaaa cctggggtta
     5341 tcatttacag gatctccaaa tcaacgatat tactttcgat aaattgatcc tggactccat
     5401 gagcagggta gtagcttcca ataacctgaa agccgctgct gaaaatgaag gacaggcatt
     5461 gctcatcacc aaaacaaaag ccgccgaagc agaaggtaat gccatcaaga tctccgccga
     5521 ggcagagaaa gaagcggccc gtctccgcgg ccagggtgtg gccctcttcc gccaggaagt
     5581 agccaagggt atgaccgaag cggctgaaca aatgaaacag gccaatctcg ataccaatgt
     5641 gatcctgttc agcatgtgga ccgaagccat aaagaatttt gcggaatacg gcaaaggcaa
     5701 tgtgatcttc ctggacggca gtccggacgg tatggaagga actatgaaac agatacaggc
     5761 gctgatggtg aagcaggcag gagtagggaa gtagtcggga gttagtagtt agtagccggg
     5821 agtctttgtt ttattaaaag accttgaaga ctggcttctt tagataaaaa ggtcccgggg
     5881 aatcatttca tcgagcaaat gataccccgg gaccttttcc atgggggtaa tgggttggtg
     5941 ttatcaacat ccgaaatcga atcttaaggt tggggtattg ggttggtgtt atcaacatcc
     6001 gaaatcgaat cttaaggttg gggtattggg ttggtgttat caccaaccca atatccctgt
     6061 taaagatctc taaatcttgc cccctccata caacgtggca cgctttttcc cgttcttacc
     6121 cccaaatact gacaggtgtt gaaaacaaac aaaaaccatt ttaaccgtct tacattactt
     6181 ttacccacct aaagagacca atatgatcga cttattgcag attaccgatt ctttgacaaa
     6241 agccacacag agcctgtccg gggatgccaa tcacgatgga caattgagtt ttttcgaatt
     6301 attgcagatg ggcggctgga tcatgattcc cctgggagcc atgatgctgc tggggattta
     6361 catttttgcc gaacggtata ttgccatccg gaatgcggcc aagatcgatg cgaatttcat
     6421 gaacatcata agggataata ttatcagcgg caatgtaact gccgcccgga attttgccaa
     6481 gaataccaat aacccggtag ccaggatcat tgataaaggc atccagcgga tcggtaagcc
     6541 gattgatggc attgaaaaaa gcatggccaa tgtgggtcag ctggaaatgt acaaactgga
     6601 gaaaaacctg gatat
//
LOCUS       DAOPMW010000164         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13336_length_6584_cov_6.653699, whole genome shotgun sequence.
ACCESSION   DAOPMW010000164 DAOPMW010000000
VERSION     DAOPMW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13336_length_6584_cov_6.653699"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="PLU11_08815"
     CDS             <1..1623
                     /locus_tag="PLU11_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPN59261.1"
                     /translation="LDYKQPLLAQLVPLLAGQFAHVFPELQQQVDFVSKVVKEEEEAF
                     LRTLEKGLKRVDDLIAQGSISGKDSFELYDTYGFPLDLTQLIAAENNLKVDEEGFKAE
                     MQQQKDRSRAATTVDAGDWIELKGGANQFVGYDSLEGVTEVLKYRKVKAKGKEGYQLV
                     LEQTPFYAESGGQVGDKGWLTIGGTEVAVVDTKKDNDLIIHFTETLPAEVSGKVNAKV
                     DAAKRKETTLHHSATHLLHAALREILGNHVAQKGSLVNEERLRFDFSHFAKVTEEEMA
                     RIETIVNEKIRENIPVVIKSLPKEEALKLGAMALFGEKYGDVVRVVIMDPAYSVELCG
                     GTHVGATGELGLFKFTGESAVAAGVRRVEAVCGKQAELFVNEQGQLISSLKEVLKNPK
                     DLQKAVESLQAENTELKKHAEAMEARQLVGIRNELLQKDEIINSVNFIGDIVEVSNPD
                     ALKKLCFDLKNHLRDHVAVLCANIGGKPFVAVGISETVTVAKNLDAGKIIKEIVAPLI
                     KGGGGGQKTLATAGGQQVDKLQEVIEAVKKLL"
     gene            complement(1627..1770)
                     /locus_tag="PLU11_08820"
     CDS             complement(1627..1770)
                     /locus_tag="PLU11_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59262.1"
                     /translation="MQYFISISIISICLVVIFWGLRTYKENRELEKQQQKYKEDDDFR
                     LYH"
     gene            1865..2377
                     /locus_tag="PLU11_08825"
     CDS             1865..2377
                     /locus_tag="PLU11_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN59263.1"
                     /translation="MHVIIETERLILRQFEPGEGQLIYTLNEDPEVTLYTGDPVRDLA
                     HANEVLEQVILPQYALYNHGRWAVHTKPGMEFIGWCGLKYRPELDEIDLGYRFMKTAW
                     GKGYASEAAMASLRYGFEKLGLNRIVGRAMPGNIASLKVLEKCGMKYIGEEMVDGHEA
                     RTYEIVGSNY"
     gene            2665..3720
                     /locus_tag="PLU11_08830"
     CDS             2665..3720
                     /locus_tag="PLU11_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPN59264.1"
                     /translation="MRMKRIQLWMPALLLALCLPAFLMAQPEEKIKEKKKEKKEVQQI
                     TITRKGDKKDKIVVEISEDKITINGKPIEEYKDADVKVMLNNLKDVESLSMTRGPRGG
                     TMTFNNNGNTMLFRSDDSRAMLGVTTNSDEKGAKVTDVTKESAAEKIGLKEGDIITRI
                     NEAEIKNPDDLSSAINKQKPGDKVTVQWLRDKKEQKATAELGKWKGMSSFGSTSPLLR
                     EFRVENDGHSPAFPDMRELQELNRFKVFEAPMGQGWSWSGNGPKLGLSVQDTDDGKGV
                     KVIEVDQESNAAKAGLKEEDLITEIDGKAVNSTDETVRIIKESKEKTSIMVKLSRGGK
                     TMNIEVKMPRKIKTADL"
     gene            3867..5315
                     /gene="gatB"
                     /locus_tag="PLU11_08835"
     CDS             3867..5315
                     /gene="gatB"
                     /locus_tag="PLU11_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPN59265.1"
                     /translation="MENSGTYEIVVGLEVHAQLLTQSKLFCGDSIAYGAEPNTHVSPI
                     TLGHPGTLPKMNRRAIEHAIKMGLACHCEIEKHNYFARKNYFYPDLPKGYQVSQHTTP
                     ICKNGYVKIKTTEGEKKIRLNRIHMEEDAGKSLHDVDEQNTCIDYNRAGTPLVEIVTE
                     PDIRSSEEAFAYLTEVRKLVRYLDICDGNMEEGSLRCDANISVRKKGDPKLGTRVEVK
                     NLNSIRNVKRAIEVEAARLINLLENGEVIRQQTRSFDANNNTTFSIRDKEDADDYRYF
                     ADPDLTPFHLEDAFIESIRAAIPVLQEERIKKYMALQLTEYDAMVLTEDKLLADYFEA
                     VISHTPNSKAAANWILGPVKTWLNENNQEIDEFPLAPVQLAKLIQLVDAGKLSFSMAS
                     SRLFTLLMQSPEKDPEQIATDNNLLQQSDASELVPVVDAVLEKLAAKVAEYQKGKKGL
                     LALFVGEVMKQTKGKADPKMTNDLLLERLNSK"
     gene            5315..6490
                     /locus_tag="PLU11_08840"
     CDS             5315..6490
                     /locus_tag="PLU11_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN59266.1"
                     /translation="MRLVFVVIAAMSLLTSCKGKKTGSQFEVRGTITNNTARMIYLEE
                     VPMIPQNGVILDSAELGKDGKYKLTAPLSESRVYNLRLDQNEYPIAAIINDVSSISLD
                     ATFNKENNQFVESYEVKGSPVSLQLKDFMTGFNSRLQTIFPLARRADSLMKQNAPDSL
                     FVPLNAEGMRISGEMKTFTTTTIKGSKNPALSIMIVGYYQSMAGNKGFGLQPLSNEEV
                     TGIVNELAVAFPNHQGVASVKAILEKEEEASPNLIGMSAPEISLPDPSGNTIKLSSFQ
                     GKYVLVDFWASWCGPCRMENPNVVNAYNKYKNKNFTILGVSLDRPGQKEAWVKAIMDD
                     KLTWPQVSDLAFWSSPVVALYGFNGIPFNVLVDPQGKIIAQSLRGAALEAKLAELLN"
BASE COUNT         1937 a         1374 c         1717 g         1556 t
ORIGIN      
        1 ctggattata agcagcccct gctggctcaa ttggttccgt tactggccgg ccagtttgcc
       61 catgtgtttc ccgaacttca gcagcaggtg gattttgtga gcaaagtggt caaagaggaa
      121 gaggaagcgt ttttgcgcac attggagaaa gggttgaaaa gagtagatga cctgattgcc
      181 cagggcagta tttccggtaa ggattccttt gaattatatg atacctatgg tttcccgctg
      241 gatctgacac agctgattgc cgccgagaat aacctgaaag tggatgaaga aggctttaaa
      301 gccgaaatgc agcaacagaa agaccggagc cgggcggcca ccacggtgga tgccggtgac
      361 tggatcgaac tcaagggtgg tgccaaccag tttgtgggct atgactcact ggaaggggta
      421 accgaagtat taaaataccg caaagtaaaa gctaagggca aggaaggata ccagctggtg
      481 ttggaacaga ctccttttta tgccgagagc ggcggacagg taggggataa gggctggtta
      541 acgatcgggg gaacggaagt ggccgtagtg gatacgaaaa aagataatga cctgatcatt
      601 cattttacag aaaccttgcc agcagaggta tccggtaagg tgaatgcgaa agtggatgcg
      661 gcaaaacgca aagagaccac cctgcatcac tcggccacgc acttattaca tgcggccctg
      721 agagagatac tgggaaatca tgtggcccag aaaggatcat tggtgaatga ggaacgttta
      781 cgttttgact tctcgcattt cgccaaggtt accgaggaag aaatggcccg gatcgagacg
      841 attgtcaatg aaaaaatccg ggagaatatt ccggtggtta taaaatcatt gcccaaggaa
      901 gaagcgctga aactcggtgc catggctttg tttggcgaaa aatacggcga tgtggtgagg
      961 gtggtgatca tggatcccgc ttattcggtc gaattatgcg gcggtaccca tgtgggcgct
     1021 acgggcgaac tcgggctctt caaattcacc ggtgaatcag ccgtggctgc gggtgtacga
     1081 cgggtggaag cggtttgtgg taaacaggcc gagttgtttg tgaatgaaca agggcaactg
     1141 atcagcagtc tgaaagaagt actgaaaaat cccaaggacc tgcagaaagc ggtggaaagt
     1201 ctgcaggcag aaaatacgga actcaaaaag catgcagaag cgatggaagc ccgtcagctg
     1261 gtgggtatcc gcaacgagtt gttgcagaaa gatgaaatca tcaacagtgt gaactttatc
     1321 ggggacattg ttgaagtcag taatccggat gccctgaaaa aattatgctt tgatttgaag
     1381 aatcacctgc gcgatcatgt ggccgtgctt tgtgctaata tcgggggtaa gccttttgtg
     1441 gcggttggta tttcggaaac ggtgactgtc gccaagaacc tggatgccgg taagatcatc
     1501 aaagagattg tggcccccct gatcaagggt ggaggcggtg gacagaaaac actggctacg
     1561 gccggtggcc agcaggtcga taaattgcag gaagtgattg aagccgttaa aaaattattg
     1621 taaggttcag tggtataatc tgaagtcgtc gtcttccttg tatttctgtt gttgtttttc
     1681 cagctccctg ttctctttgt aggtccggag accccagaaa atcacaacca ggcaaatgct
     1741 aataatggaa atggagatga agtactgcat ggaaaccgtc acttgaatta aagcactaat
     1801 tactgcaatt ctgcggaaca cacaatacca ctttccggtt agtaaaacta ctcttcgttt
     1861 atctatgcac gttatcattg aaacagaacg gttgatcctt cgccagtttg aacccggcga
     1921 aggacaattg atttataccc tgaatgagga cccggaagtc acactgtata ccggcgaccc
     1981 ggtgcgtgac ctggcccatg ccaatgaggt actggaacag gtgatcctgc cgcagtatgc
     2041 cttgtataat catgggcgct gggcggtaca taccaaaccc ggcatggagt tcattggctg
     2101 gtgcgggtta aaataccgtc ccgaattgga tgagatcgat ctcggttacc gctttatgaa
     2161 aaccgcctgg ggaaaaggct atgccagtga agcggccatg gcttcactcc ggtatgggtt
     2221 tgaaaaactg ggtctgaata ggatagtagg acgggccatg ccaggtaata tagcttccct
     2281 taaagtattg gagaaatgtg ggatgaagta tatcggagag gagatggtgg atgggcatga
     2341 ggcgaggacg tatgagatag ttggtagtaa ttattgatta ttgagtcgct tgaatttgga
     2401 gcgtcttttt aaacagggaa gggtcttaat aactgattat taattattta cctcctagtg
     2461 agaattttgc agcttataaa cgacccccaa taattaataa taaataatca ataatccctt
     2521 ctgtcaagct atttcaaatt ttttgtaggc ttccattaaa gccccctccc tgtatttaac
     2581 atttccgaaa catttcgtat ttccaaattt ctctttatat ttgatctacg aaacttaacg
     2641 tagataaaaa ataaaacaga ttttatgcgt atgaaacgga ttcaactatg gatgcctgcc
     2701 ctgctgctgg ccctttgttt acctgccttt ctgatggccc agccggagga aaagatcaag
     2761 gaaaaaaaga aagaaaaaaa ggaagtccag cagatcacga tcacccgtaa aggcgataag
     2821 aaagataaaa tcgtggtgga aatcagtgaa gacaagatta ccattaacgg aaaaccgatc
     2881 gaagagtata aggatgccga tgtaaaagtg atgctgaata atttaaaaga tgtggaatcc
     2941 ctgtccatga cacgcggtcc gcggggcggc actatgactt tcaataataa tggaaatacc
     3001 atgttgtttc gctctgacga tagccgggct atgctgggtg taaccaccaa tagcgatgaa
     3061 aaaggagcga aggtgactga tgtaaccaaa gagagtgctg cagaaaaaat tggtttaaaa
     3121 gaaggggata ttatcacccg tatcaatgag gccgagatca aaaatccgga tgatctttct
     3181 tccgccatca acaaacaaaa acctggtgat aaagtaacgg tacaatggct gcgtgataaa
     3241 aaagaacaga aagccactgc tgaactcggt aaatggaagg gcatgtcctc ctttggatcc
     3301 accagtcccc ttttaagaga attcagggta gagaatgatg gtcatagtcc cgcttttccg
     3361 gatatgcgcg aattacagga actgaaccgt ttcaaagtct ttgaagcccc gatgggacag
     3421 ggatggagct ggagcggtaa tggccccaaa ctgggacttt ccgttcagga cactgacgat
     3481 ggtaaaggag tcaaggtgat tgaagtggac caggaaagca atgcggccaa agcaggtttg
     3541 aaagaagagg atctgatcac cgagatcgat ggcaaagcag taaacagtac cgatgagact
     3601 gtaaggatca ttaaagaaag caaggagaaa acctccatca tggtaaaact gtcaagaggc
     3661 ggaaagacca tgaatattga agtaaaaatg ccaagaaaaa taaaaacagc cgacctgtaa
     3721 acagaacctt tctgcaggag ctctaaactg aggctgctaa tgacgtatgt caccctgagt
     3781 gtaccgaagg gggcatacgt cattagcagc ttctcttttt tacgccgctg tactggccca
     3841 aatgggctat ttttgccctt cataaaatgg aaaacagcgg tacatacgaa atagtggtgg
     3901 gactggaagt gcatgcccag ttgctcaccc aaagcaagtt attctgtggc gactctattg
     3961 cctatggggc ggagcccaat acccatgtca gtcccatcac cctcggtcat cccggtacct
     4021 tgccaaagat gaaccgccgg gccattgaac atgccattaa aatgggtctg gcctgtcatt
     4081 gtgaaattga aaagcacaat tatttcgccc ggaagaatta tttctatccc gacctgccca
     4141 agggctacca ggtatcgcag cataccaccc cgatctgtaa gaacgggtat gtaaaaatta
     4201 agacaacaga aggagagaag aaaatacggc tgaaccggat ccatatggaa gaggatgcgg
     4261 ggaaaagcct gcatgatgtg gatgaacaaa atacctgtat cgattataac cgggccggca
     4321 ccccgttggt ggagattgta acggagccgg atatccgcag cagcgaagaa gcgtttgctt
     4381 atctcactga agtgcgcaaa ctcgtgcgct acctggatat ctgcgatggc aatatggaag
     4441 aaggaagcct tcgctgtgat gccaatatat cagtacggaa aaaaggcgat ccgaagctgg
     4501 gcacccgcgt ggaagtaaag aacctcaatt ccatccgtaa tgtgaaaagg gccattgaag
     4561 tggaagcggc ccgtctgatc aacctgctcg aaaatggaga agtgattcgt cagcaaaccc
     4621 ggagttttga tgcgaataac aacactactt tttcaatccg ggataaagaa gatgcggatg
     4681 attaccggta tttcgcggat ccggatctga cacccttcca tcttgaagat gcttttattg
     4741 aatccattcg tgcggccatt ccagtgttac aggaggaacg gatcaaaaaa tatatggctt
     4801 tacagctgac cgagtatgat gccatggtgc tgacagaaga taaactgctg gccgattatt
     4861 ttgaagccgt aatcagtcat acccccaaca gtaaggctgc ggccaactgg atactgggtc
     4921 cggtaaagac ctggctgaat gaaaacaacc aggagattga tgaattcccg ttggccccgg
     4981 tgcaactggc aaagctgata caactggtgg atgccggcaa actcagtttc tccatggctt
     5041 cttcccgttt attcacgttg ctgatgcaat caccagaaaa ggatccggaa cagatcgcta
     5101 ctgataacaa tctgctacag caatccgatg cctcggagct ggtacccgtg gtggatgccg
     5161 tactggaaaa acttgccgcc aaagtggccg aatatcaaaa aggaaagaaa ggattactgg
     5221 ccctttttgt aggggaagtg atgaagcaga ccaaagggaa ggccgatccc aaaatgacca
     5281 atgatttatt actcgaacgt ttaaattcaa aataatgaga cttgtatttg tagtgattgc
     5341 cgccatgagc ctgttgactt cctgtaaagg gaaaaagacc gggagccagt ttgaagtgag
     5401 aggaacgatc acgaataata ctgcccggat gatctacctg gaagaagtgc ccatgatccc
     5461 tcagaacggg gtcatcctgg attctgctga actgggcaaa gacgggaaat ataaactgac
     5521 cgccccgctc tccgaatccc gggtgtacaa tctccgcctg gatcagaacg agtatccgat
     5581 tgctgcgatt attaatgatg tttcatccat cagtctggat gcaactttta ataaagagaa
     5641 caaccagttt gtggaatctt atgaagtgaa aggatcaccg gtaagtctgc aactgaaaga
     5701 tttcatgacc ggatttaatt caagattgca aactatattt ccgctggccc gccgggctga
     5761 cagtctcatg aagcaaaacg ctccggatag tttatttgtc ccattgaatg cagaaggcat
     5821 gcgtatatcc ggtgagatga aaacctttac cactacgact attaagggca gtaagaatcc
     5881 ggctttgtcc attatgatag tgggttatta tcaaagcatg gccggcaata aaggatttgg
     5941 tctgcagccc ctcagtaatg aagaagtgac cggaatcgtg aatgagctgg cagttgcttt
     6001 tcctaatcac cagggagtag ccagtgtcaa agcaatattg gaaaaagaag aggaagcttc
     6061 tcccaacctg attgggatga gtgcaccgga gatcagtctg cccgatccct ccggtaatac
     6121 catcaaactg agttcattcc agggtaaata tgtgctggtg gatttctggg ccagctggtg
     6181 tggcccctgc cggatggaaa atcccaatgt ggtaaatgcg tataataagt ataagaataa
     6241 aaacttcacc atcctggggg tgtcgctgga ccggcccgga caaaaagagg cctgggtaaa
     6301 agccatcatg gatgataaac tcacctggcc acaggtaagt gatctggcat tctggagcag
     6361 tccggtagtg gccctttacg ggttcaatgg cattcccttt aatgtgttgg tggatcccca
     6421 gggaaagatc attgcccaat cgcttcgtgg cgcggcgctg gaagccaaac tggctgagtt
     6481 gctgaattaa ccgtttgcag attcttgcgg taactgaaag acagaatttg taaatttatc
     6541 aatgcggttt ttaaggctga aaccagccat taccctcata ttgc
//
LOCUS       DAOPMW010000165         6571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13376_length_6571_cov_7.503683, whole genome shotgun sequence.
ACCESSION   DAOPMW010000165 DAOPMW010000000
VERSION     DAOPMW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_6571_cov_7.503683"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PLU11_08845"
     CDS             complement(<1..1512)
                     /locus_tag="PLU11_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59267.1"
                     /translation="MRKLFLLSVLYLYTISLLAQQFGGNPPSLKWRQINTDTVRIIYP
                     VGLDSQANRVAAMVHILAARKPVSLGDQQKKVDIVLQNQTVIANGYVQLGPYRSEFFL
                     TPDMNNFGQGTVSWTDQLALHEYRHVQQFNNFNNGLSKAMKFLFGEEGYALAINASVP
                     DWFYEGDAVYQETVLSQQGRGRIPLFMNAYPSLWQSGKKYNWMKLRNGSLKDYVPSHY
                     HLGYLLVNYGRIKYGEDFWAKVTRDASAFKSLVYPFQAAVKTHAGVDYATFRKEAFDF
                     YRSGTRQAVENKGASNLFPVRKNYVTNYFFPYSAGKDSLVYLKSSYRHRPAFFLRDAA
                     GEKRIRTRDISFDEQFSYRNGKIVYAAYETHPRWGWQDYSVIKLLDIRTGQKKRLTRN
                     SKYFTPDISADGTRVAAVQVNTDGKSELHVLDANSGEVKQRIHSAEISLFTDPKFIDA
                     QTLITVVRLKDGRTALATADIATGVTIRLTPLTFNIIGYPTVREGVVYFTASYQ"
     gene            1622..2095
                     /locus_tag="PLU11_08850"
     CDS             1622..2095
                     /locus_tag="PLU11_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPN59268.1"
                     /translation="MAEDLTISIGDKADQYQSLIPQIQGLLEGENDLVANLANVAAAL
                     KEQFGWLWVGFYLVKGDELVLGPFQGPVACTRIRKGRGVCGSSWATARTLVVPDVEQF
                     PGHIACSSSSRSEIVVPIIRNGAVLGVLDADSAEWNQYDAIDQEYLEKITALILW"
     gene            2089..2370
                     /locus_tag="PLU11_08855"
     CDS             2089..2370
                     /locus_tag="PLU11_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPN59269.1"
                     /translation="MVNELSSIRLIVKGKVQGVFFRKHTRQKALDLGLEGEVMNQPDG
                     SVCVLATGTKDQLEALTAYCRTGPPAASVSSLQVEIIGFKKFSSFRILK"
     gene            2478..3098
                     /locus_tag="PLU11_08860"
     CDS             2478..3098
                     /locus_tag="PLU11_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59270.1"
                     /translation="MLKLPNSGRSNFVLPAIAALFFMTILFSSCADDPKAPLANDAPE
                     KKEFKMNCVKLTRTQMQAWVDSGWTRPGTAGKINTLLLQFYSVDAAGIASNMQLTSYP
                     GSSATDFKISGNALLAIDTACKAKTFSGKIILANNQISFDALKVFNADGTLKEFDFIR
                     FTPQHFSQISEYITFNAEVIKEGVTKSDLGGPTKPCPPACCPPICE"
     gene            3109..3798
                     /locus_tag="PLU11_08865"
     CDS             3109..3798
                     /locus_tag="PLU11_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59271.1"
                     /translation="MYTYYRLFLNWSEVWPLIIALLIFVFFKQRENITIIFWLLVISL
                     LLHTVATYISLFTYRVPEPYKNNNILYNLLAIIKPVMAGLYLLKLKQLQQYKYLKIVF
                     LLFLLFTLFNFLFIESIFTYSSSMVLAQSAFLLIFTLTFFLDAMIDDEIPLPLSHPAY
                     FICTAIGLSESINFFINLFIFQVFKTSYELSVLTMNISSYAFIIYGLILATGIFLNRE
                     KPRFSPFSQSI"
     gene            3795..4580
                     /locus_tag="PLU11_08870"
     CDS             3795..4580
                     /locus_tag="PLU11_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN59272.1"
                     /translation="MNNNITLLVIMGIVVMLGLMLSVLLAVMFNFRKKNQHRRAMEQL
                     KEMQQNQLIEAAIRSEETERHRISDTLHDEVGAILSSAKLHLLGIKQTTLDEKDKKLH
                     EKGAGLLNDVIKRVRGISHNLHSNILKEFGLNEAIRHFLQQVTGGTLISTDTRLDEAY
                     KGSTPEHDMSLYRIIQELINNTIKYAGATKLEINSTLVGQELVVSLIHNGNGLSQEDF
                     ETFSFRKEGLGLKNIRNRILLLKGNISFFKTSETSTVRVSLPI"
     gene            4597..4965
                     /locus_tag="PLU11_08875"
     CDS             4597..4965
                     /locus_tag="PLU11_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59273.1"
                     /translation="MPKFKIAIADDYPIYRDGLQVTLSSDENLEVIFEADNGQELLDK
                     MDISVPDLVIMDYKMPVMDGMVATRLIKEKHPAVKVLVISMFHDEKFINHLMDNGADG
                     YLLKDAEPEQIMATIHQLLY"
     gene            complement(4968..5912)
                     /locus_tag="PLU11_08880"
     CDS             complement(4968..5912)
                     /locus_tag="PLU11_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN59274.1"
                     /translation="MVKEKILVIGASGQIGVELTMALRKLYGNANVIASDLREQNPLL
                     EGTGPYVSLDVMNKEMLHVQVIRQNITQIYLLAAILSATGEKNPGLAWNLNMQGLLNV
                     LDIAREEKLQKVYWPSSIAVFGPTSPRQQCPQQTIIEPTTVYGISKYAGEFWCHYFHQ
                     RFGVDVRSIRYPGLISYKSAPGGGTTDYAVEIYHEALEEKKYECFLKEDTYLPMMYMP
                     DAIRATIELMEAPASKIKIRTSYNVSGMSFSPREIGAEISKHVEGFSMTYKPDYRQAI
                     ADSWPQSIDDTVARNDWGWKEEFDLAAMTSDMLKNLRE"
     gene            6002..>6571
                     /gene="kdsA"
                     /locus_tag="PLU11_08885"
     CDS             6002..>6571
                     /gene="kdsA"
                     /locus_tag="PLU11_08885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPN59275.1"
                     /translation="MTNFLPDLFRGQTYDSSNFFLLSGPCVVESEDLVMEVAEKVSGI
                     CRNLGIPYVFKASYRKANRTSASSFTGLGDQPALEILKKVKDRYQLPVVSDIHAHEEA
                     AMAAPYVDILQIPAFLCRQTDLLIAAAETGKVINVKKGQFLSGPSMKFAAEKIRQAGN
                     EKVMLTERGTTFGYQDLVVDYRNIPWMQEI"
BASE COUNT         1754 a         1588 c         1497 g         1732 t
ORIGIN      
        1 ctggtaagat gccgtgaaat aaaccacccc ctcccttact gtgggataac caatgatatt
       61 gaaggttaaa ggagtcagcc gaatcgtcac gcctgtggct atatccgcag tagcgagtgc
      121 ggttcttcca tctttcaaac ggaccacggt aatcagcgtt tgtgcatcta taaacttagg
      181 atcggtaaac aggctgatct ccgcagaatg aatccgttgt tttacttccc cgctgttagc
      241 atccagtaca tgcagttctg attttccatc tgtattgacc tgtactgcag ccacccgggt
      301 accgtcggct gaaatatcag gcgtaaaata ttttgaattc cgggtcaacc tctttttctg
      361 tccggtccgg atatccagta gtttgatcac actgtaatcc tgccaacccc aacgcggatg
      421 ggtttcatat gccgcataaa cgatttttcc attccggtaa ctgaattgct catcaaatga
      481 gatatccctg gtacggatcc gtttttcacc agcagcatcc ctgaggaaga aagcggggcg
      541 gtgtcgataa ctggatttta aatataccag tgaatccttt cctgcactgt aaggaaaaaa
      601 ataattggtt acataatttt tccggaccgg gaaaaggttg gatgcccctt tgttttcaac
      661 agcctgcctg gtaccactcc ggtaaaagtc aaatgcctct ttccggaaag tggcataatc
      721 cacccccgca tgtgttttaa ccgccgcctg gaaaggataa acgaggcttt taaatgcact
      781 ggcatcccgg gtcacttttg cccaaaagtc ttccccgtat ttaatccggc cgtaattgac
      841 cagcaggtaa cccaggtgat aatgactcgg cacataatct ttcagggaac cattgcggag
      901 cttcatccag ttgtactttt ttccggattg ccagagcgag ggataggcgt tcataaataa
      961 tggaatccgt cccctgccct gctggctgag tacggtttcc tggtacacgg catctccttc
     1021 ataaaaccag tcgggcaccg atgcattgat cgccaatgca tagccttcct caccaaacag
     1081 gaatttcatg gctttactaa gaccgttatt gaaattgttg aactgttgta catgacggta
     1141 ttcatggagg gccagctgat ccgtccaact gacggtacct tgaccgaaat tattcatgtc
     1201 cggtgtcagg aaaaattcac tgcgataagg gccgagttgt acatatccgt tggcaattac
     1261 ggtttggttc tgcagtacga tatctacttt cttttgctga tcgcccagtg aaaccggttt
     1321 ccgggctgcc agtatatgta ccatggctgc cacccggtta gcctgactgt cgagtccaac
     1381 cgggtaaatg atccggacgg tatctgtatt gatctgccgc catttcaacg aaggcgggtt
     1441 gccgccaaac tgttgggcca ggagggatat ggtgtacaaa tacaaaacag ataaaagaaa
     1501 taacttccgc atatcggagg attttatagt gaaaggtaag tcagaaaaca gataaggata
     1561 acaactgaaa acagaatttg tgcaatccgg gaaactgaat cggggtttgt atctttgtcc
     1621 aatggcagaa gatttaacca ttagcatcgg cgataaggcc gatcaatatc aatcgctgat
     1681 tccccagata caaggattac tcgaaggcga aaacgacctc gtggccaatc tcgccaatgt
     1741 agccgccgcc ctgaaagagc agtttggctg gctttgggtg ggcttttacc tggtgaaagg
     1801 ggacgaactc gtactgggtc cattccaggg accggtggcc tgtacccgga tacggaaagg
     1861 acgcggggtt tgtggcagca gttgggcaac agcccgtacc ctggtagtgc ccgatgtgga
     1921 gcagtttccc ggacatatcg cctgcagcag cagttcccgg tcagaaattg tggtgcccat
     1981 catccgaaat ggtgccgtac tgggagtact cgatgccgat agtgcggaat ggaatcaata
     2041 tgatgccatt gaccaggagt acctcgagaa aatcacagca ctcatcctat ggtaaatgag
     2101 ttaagcagca tccgtctcat agtaaagggc aaagtgcagg gcgttttttt ccggaaacat
     2161 acccggcaaa aagcccttga tctgggtctg gaaggggaag taatgaacca accggatgga
     2221 tcagtatgtg tgctggcaac cggcacgaaa gatcaattgg aggcgttgac cgcctattgc
     2281 aggacaggac ctccggctgc aagcgtcagc agtttgcagg tggaaataat cggattcaaa
     2341 aaattcagca gtttccgcat actaaaataa tttccccgct tttaactaaa agcattgtaa
     2401 aatcagatta cactttctaa ttttagcatt ccccaccggg aactgaatct tttacttttc
     2461 atttaaaccc atttcttatg ttgaaactac cgaactccgg caggtctaac tttgtcctgc
     2521 cagcgattgc cgccctattc ttcatgacca tcctcttcag cagttgtgcc gatgatccca
     2581 aagccccact ggccaatgat gccccggaga aaaaagaatt taaaatgaat tgcgtgaaac
     2641 tcaccaggac acagatgcag gcctgggtag acagtggctg gacccgtccc ggcactgctg
     2701 gcaaaatcaa taccctcttg ctacaatttt attccgtgga tgcggcgggc atcgcctcca
     2761 acatgcaact gacatcgtat cccggctctt ccgccaccga ttttaaaatc agtggcaatg
     2821 ctctgttagc aattgataca gcctgcaagg caaaaacatt cagcggcaag atcatcctgg
     2881 ctaataacca gatcagtttt gacgccctga aagtattcaa tgcagacggc acattgaaag
     2941 agtttgactt tatccgtttt accccacagc acttcagcca gatcagcgaa tacattacct
     3001 ttaatgcaga ggtgattaaa gaaggtgtaa caaaaagtga cctgggtggc ccgacaaaac
     3061 catgtccacc tgcttgctgt ccgcctattt gcgaataaac cgggcaaaat gtatacctat
     3121 tacagattat ttttaaactg gtcagaggta tggcctctca tcatcgcatt actcatattt
     3181 gtttttttca agcaacggga gaatatcaca attatattct ggttgctggt catttctttg
     3241 ctcctgcata cggtggctac ctatatttcg cttttcacct atcgggtacc tgaaccctat
     3301 aaaaataata acatattata caatctactg gctattatca aaccagtgat ggctggcctt
     3361 tacctgttaa aactaaagca attacaacaa tacaaatacc tgaaaatcgt atttctcctg
     3421 tttctgttat tcaccttgtt taatttcctg tttattgaaa gtatattcac ttacagttcc
     3481 agtatggtac tggcccaaag tgcctttctg ttgattttca cactgacatt cttcctggat
     3541 gccatgatcg atgatgaaat tcccttgccg ttatctcacc cggcttattt catttgtacg
     3601 gccattggct tatcagaaag catcaatttc tttattaacc ttttcatttt ccaggtattc
     3661 aaaaccagct atgaattatc tgttttgaca atgaatatat ccagctatgc gtttatcatt
     3721 tatggtctta tcttagccac aggtattttt ttaaacaggg aaaaaccaag attttctcca
     3781 ttcagccagt caatatgaat aacaatatca ctttattggt tataatgggt atcgtcgtta
     3841 tgttgggact catgcttagt gttttgctgg cggtgatgtt caacttccgg aaaaagaacc
     3901 agcaccgccg ggctatggaa cagctgaaag aaatgcagca aaaccagctg atcgaggccg
     3961 caatccggag tgaagagacc gaacggcacc ggatttcaga taccctgcat gatgaagtcg
     4021 gtgctattct ctcctccgcc aaacttcact tgttgggtat aaaacaaacg accctggatg
     4081 aaaaggataa aaagctgcat gagaaaggag ccggtctcct gaatgatgtc atcaaacggg
     4141 tgcggggcat ctcccataac ctgcacagca atatcctcaa agagtttggt ttgaatgaag
     4201 ccatccggca cttcctgcaa caagtgaccg gcggtaccct catcagcacc gatacccgat
     4261 tggatgaagc gtataagggt agtacccctg aacatgacat gagtctgtac cggattatcc
     4321 aggaactgat taacaacacg attaaatatg ccggggccac caaactggaa atcaattcaa
     4381 ccctggtggg acaggaactg gtggttagcc tgatacataa cgggaacgga ttaagccagg
     4441 aagattttga aacctttagt ttccggaaag aaggactggg gttgaaaaat atccgtaacc
     4501 ggatactcct gttgaaagga aatatctcct tttttaaaac cagtgaaacc tccactgtcc
     4561 gggtcagcct gcccatttaa cctaaaccta ctcctcatgc ctaaattcaa aattgccatt
     4621 gccgatgatt atcccattta ccgggatggt ctgcaagtga cgctctcctc cgacgaaaac
     4681 ctggaagtta tttttgaagc ggataatggc caggaattac tggacaaaat ggatatctct
     4741 gtacccgatc tggtgatcat ggattataaa atgcccgtga tggatggcat ggtggccacc
     4801 cgcctcatca aggaaaaaca cccggctgta aaagtgctgg tcatcagtat gtttcatgat
     4861 gaaaagttca tcaatcacct gatggataat ggtgccgatg gctacctgct taaagatgcg
     4921 gaaccggaac agatcatggc aaccatccac caacttctct attagtatca ttcacgcagg
     4981 ttcttaagca tgtccgatgt catagcggcc aggtcgaatt cctccttcca tccccaatca
     5041 ttccgggcta cggtatcatc gatactttgc ggccagctgt cggcaatcgc ctgccggtaa
     5101 tccggcttat acgtcataga aaaaccttcg acatgcttgc tgatctccgc ccctatttcc
     5161 ctgggtgaaa aactcatccc cgatacatta taggaggtcc ggattttgat tttgctggcc
     5221 ggggcttcca tcagttcaat ggtggcacgg attgcatcag gcatatacat catcgggaga
     5281 taggtgtctt ctttcaggaa acactcatac tttttttctt ccagggcctc gtggtaaatc
     5341 tccaccgcat aatcggtggt gcctcccccg ggagctgatt tataggaaat aagtccggga
     5401 taccggatac tccgcacatc cacgccgaat cgctggtgaa aatagtgaca ccagaattca
     5461 ccggcatact tactgattcc atatacggtg gtaggttcaa taatcgtttg ctgtggacat
     5521 tgctgacggg gagaagtagg accaaatacg gcaatagaag aaggccagta cactttctgt
     5581 agtttttcct ctcttgcaat atccagcaca ttgaggagcc cctgcatatt caggttccag
     5641 gccagtcccg ggtttttctc cccggtagcc gagaggatcg cggccagcag atagatctgg
     5701 gtgatattct gacggatcac ctgtacatgc agcatttctt tattcatcac atccagggat
     5761 acatagggac ccgtgccttc cagcaacggg ttctgttccc gcaggtcgga agcgatcaca
     5821 ttggcattcc cgtacagttt acgaagggcc atggtcagtt ccacccctat ctgaccggag
     5881 gcacctatga ccaggatttt ctcttttacc atagcaagcg tttagaagac aaagatgaag
     5941 gttcgttttt caaattccaa cttaaaaagc aaatccggga acttcgtttt atttttgccg
     6001 catgacaaat tttcttcccg atctgttccg tggacaaacc tacgattcat ccaatttctt
     6061 cctgctgtcc ggcccctgtg tggtggaaag tgaagacctg gtaatggaag tggcggagaa
     6121 ggtttccggc atctgccgca acctgggtat cccctatgtt tttaaggcct cgtaccggaa
     6181 agccaatcgc accagtgcca gttcctttac cggcctgggt gatcagcctg cgttggagat
     6241 actgaaaaaa gttaaggacc ggtatcaatt gccggtcgtt tccgatattc atgcgcatga
     6301 ggaagcagcc atggcggcac cttatgtaga catcctccaa atacccgcct ttctttgccg
     6361 gcagaccgat ttgctgatcg cggcggcgga gacggggaaa gtgatcaatg taaaaaaagg
     6421 acaattcctg agcggacctt ccatgaaatt tgcggcggag aaaatccgcc aggccggcaa
     6481 tgaaaaagta atgctgacgg aacggggaac cacttttggc taccaggacc tggtggtgga
     6541 ttaccggaat attccctgga tgcaggaaat c
//
LOCUS       DAOPMW010000166         6553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13441_length_6553_cov_5.023238, whole genome shotgun sequence.
ACCESSION   DAOPMW010000166 DAOPMW010000000
VERSION     DAOPMW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13441_length_6553_cov_5.023238"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..303)
                     /locus_tag="PLU11_08890"
     CDS             complement(22..303)
                     /locus_tag="PLU11_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPN59276.1"
                     /translation="MAKKVSVTPLHDRVIVKAAAAEEKTAGGIIIPDTAKEKPQRGTV
                     IAAGPGKKDEPVTVKTGDTVLYGKYAGTEIQIEGEDYLIMRESDILAIV"
     gene            complement(564..1076)
                     /locus_tag="PLU11_08895"
     CDS             complement(564..1076)
                     /locus_tag="PLU11_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59277.1"
                     /translation="MKKQPFHAILLLFAGLFFSICWACSQSKSIVSKTYGFYSERQPG
                     NIPVDPSGNPLPGFGVDTILTVYVTSSGKELQWDTAWSGYKVYTIMAARLTETVIEAG
                     SEKEDNQLTIIKAGKGLVIWRLDLVPLDPMPGWHGDYLKGIRIKGEKAGKPFEVKIGK
                     LVQLRVPPSV"
     gene            complement(1159..4917)
                     /locus_tag="PLU11_08900"
     CDS             complement(1159..4917)
                     /locus_tag="PLU11_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59278.1"
                     /translation="MLLLSGCMEMNPGTEPNTIQITAKDRFVKKPKAQDGISDAQRME
                     FDITADRTLGYIPRNRLIEATEQLKAARASGNYTNRLNALSWAERGSSSDAVGPSNGN
                     GRLGSSVTSGRMRAIWVDLADVTNKTVWVGGVDGGVWKTTDITAASPNWTPANDFLGN
                     LAIADITQDPTNTDVMYFATGEKAINIDAVAGGGIWKSTDHGITWSLLPGTTTFWNCS
                     RIVCDASGNLYIAAVASLVGIPGSTGGIYRSTDGGATWSANLSPTGLTTRVSEMVISS
                     TGRMHITCGYYNTSAATAGYRYTDNPATVTSATWTAPTTAFTSLRYNCDIAVNGNTVY
                     ALPSSSAFQTPQVWKSIDGGDNWAITGTSPPIAGSAPVSSGQAWYCIAIGVDPTDANK
                     VMVGGLNSYISTDGGATWSVNSVWVTGIPGSTNYIHADHQIIVWNNNQVLDGGDGGLF
                     YSADDGVTFADRNVGLRLKQFYACAIHPSTTNYFLAGAQDNGVHQFNGAGITSSVEVT
                     GGDGAYVHIDEDQPQYQFGAYVYNQYYRSINSGASWSSIAYSGSAGQFINPTDYDDIN
                     NNFYGAYNAGTYIRWINAISGATAFANTVPAFSGGTVSYTGVSRYTSNRVYFGTNNGR
                     IVRVDNAHSATFTATDITGSTMNVANVSCVAQGTTENNLLATFSNYGATHVWLSTTGG
                     GAAGWTDITGSGLPDIPVRWAMFYPEDNTKAILATEMGIYETSLLNGASTVWVQNSSF
                     PTVRTNMLQYRFSDNTILASTQGRGLWSSVIPPATPYIRFASSYNYSPLRTEATGSTS
                     GCRNFTDYTLNMHIDKAPVGTATVTLSIGGGATATQGVDYDFTTNGNFAAPSSVLTFN
                     NGATADQSITIRIYNDAETESTTPEFFTINYLVTGTTDAMAAPGSGSYTYYIGENDAA
                     PTSTPVETVLNNNKTEQVASNGSYYFYSSGTGNIISSITGATANLGCVSANVFEAGTT
                     WQSFAGGQRSQKVVEVTPTSNSGATYTIGIYMTAAELAGKPPASLKIAKTTAATMAGA
                     NSSNTVLVTTSFAAYGSDFLFTATFTGFSKFFLADNNVVLPVDLLSFSGYLNTQLYGT
                     LQWQTTNQFNLDKFEIERSYDGVQFTRAGTVTALANPANVQEYSFTDPLSAKPVNFYR
                     LKMVDMDGRSKFSAIIKINNNKQIQFARLIQNPVQDQIALQIRNDNRENLTAALYNQQ
                     GQRVYNWNLGRQQGSLILPVNQLKLTHGIYILQLNAGEQTASLRLLKN"
     gene            5099..6025
                     /gene="nusB"
                     /locus_tag="PLU11_08905"
     CDS             5099..6025
                     /gene="nusB"
                     /locus_tag="PLU11_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPN59279.1"
                     /translation="MISRRNIRVKVMQTLYTVSLAENGAKPGEAQKLLQQHFDETRSL
                     LVYLTWFLTEVTRYAETDAHQRASKHLPSADDLNVNTKLAGNELLWKMLEDGELKEQF
                     AKEKPELMANRDLIRKTYITLAETTDYKTYISKGARDRQEEKKILEFILNDLMLANEV
                     FVSHIEEHFSNWDDDGEMVVQLLSGYLQKPGSLHFKQMLSPDKESFAKTLLKTVQDKN
                     EHLQTLIIPKLKNWDPERIAQLDMIMMKMGVAEFLYFETIPPKVTINEYIDLAKDYST
                     QQSGQFVNGILDNIHKELVQQGKMEKVDYKKP"
     gene            6090..>6553
                     /locus_tag="PLU11_08910"
     CDS             6090..>6553
                     /locus_tag="PLU11_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPN59280.1"
                     /translation="MKKTALIVLAALSLIACKEMDKKVAPVVKMSKEEIEKAVTDTTN
                     FTTITWLDSTTQSLGKLKKDKEIEITWRFKNTGDKNLIIENVTASCGCTIPEKPEQPF
                     APGQEGVIKAKFNGSGSGSITKQVHVIANTKPMKDQTLTFTGVDLSLVLCWQS"
BASE COUNT         1770 a         1562 c         1498 g         1723 t
ORIGIN      
        1 agctcattgc tcattgcttt cctaaacgat ggcgaggata tctgactccc gcataatcag
       61 gtagtcttcg ccttcaatct ggatttcggt acccgcgtat ttaccatata atacggtatc
      121 accggtttta acagtaacgg gttcgtcttt cttaccagga cccgctgcga tgacggttcc
      181 acgttgaggt ttttcctttg ctgtatccgg gataatgatg ccaccggctg ttttttcttc
      241 tgctgcggca gcttttacaa ttaccctgtc atgaagaggg gtaacactga ctttcttggc
      301 catagttgta ttgattttga tgttttttga tgattttttg tcgcctcaac tcagttccgg
      361 agaaagggaa ttgagtgaga gcacgcaacc tgacaggttt aaactgcttg tggatgcggg
      421 cgcaaaggta cacgaaatac tatgccgggg gcagaaaaaa gccatttttt cagccccgga
      481 gaggttaagg tgctggaaaa actcacatta cggcaggtga ccgacaaaat tgacagtagg
      541 aaaccgggaa tggcaattgc cttttaaact gaagggggaa cccggagttg taccagtttt
      601 ccaatcttaa cctcaaatgg ttttccggcc ttctcccctt ttatccgtat gcctttgagg
      661 taatctccat gccatccggg cattggatca agcggcacta agtcaagtct ccatataacc
      721 aaacccttcc cggctttaat gattgttaat tgattgtctt ccttttcact gccggcctcg
      781 atgaccgttt ccgtcaacct ggccgccatt attgtataca ctttataccc agaccaggcg
      841 gtatcccatt gcaattcttt tccagaactg gttacataaa cagttaaaat ggtatccacc
      901 ccaaacccgg gtagcggatt acccgagggg tctaccggaa tattcccggg ctggcgttct
      961 gaatagaaac catatgtttt actgactatt gactttgact gtgaacaggc ccagcaaatt
     1021 gaaaaaaaca gacctgcaaa caagaggagg atagcatgga atggctgctt tttcataatt
     1081 gtatttcgtt aaaggtctcc aaaaaaccaa tccgctacgt tacccgcagc ggactggtta
     1141 attttatttc aggatcgttt aatttttcaa cagccgcaaa ctggcggttt gttcgccggc
     1201 gttcaactgt aaaatataaa taccatgggt cagtttcagt tgattgacgg gaagaatcag
     1261 gcttccctgt tgccggccca ggttccagtt ataaacacgc tgtccctgct ggttatacag
     1321 ggcggcggtc aggttctccc ggttatcatt ccggatctgt aatgctatct gatcctgtac
     1381 cggattttga atcaggcggg caaactggat ttgtttatta ttattgatct taataattgc
     1441 gctgaattta gaacgcccat ccatatcgac cattttcaac cggtaaaaat taaccggttt
     1501 tgctgaaagc gggtctgtaa atgaatattc ctgtacattg gccggattgg caagagccgt
     1561 aaccgttccg gccctggtaa attgaacgcc atcataactc cgttctattt caaacttatc
     1621 caggttaaac tggttggttg tctgccattg taaagtgccg tacaattgcg tgttcagata
     1681 accactgaaa gataacaggt ctaccggtaa caccacatta ttatccgcga ggaaaaattt
     1741 tgaaaatccg gtaaatgtcg cggtaaacag gaaatcactg ccataggcgg caaaggaagt
     1801 ggtgactaaa acagtattgg acgaattggc tcctgccatg gtggcggcag ttgtttttgc
     1861 aattttcaga ctggcgggtg gctttccggc cagttccgca gcggtcatgt agatcccgat
     1921 tgtataggtc gcccctgaat tactggttgg ggttacttca accactttct gggaacgttg
     1981 tccgccagca aaactctgcc aggtggtacc ggcctcaaat acattagcgc tgacacaacc
     2041 caggttggcc gtggcaccgg tgatgctgct gataatattg ccggttccac tggaataaaa
     2101 ataataggag ccgtttgatg ctacctgttc cgtcttatta ttatttagta cagtttccac
     2161 cggtgtcgat gttggtgcgg catcattttc cccgatataa taagtataac tgccactgcc
     2221 cggtgccgcc atggcgtcgg ttgttcccgt taccagataa ttaattgtaa aaaactcagg
     2281 ggtagtgctt tcggtttctg catcattata gatacggata gtgattgatt gatccgccgt
     2341 tgccccgtta ttaaatgtca gcacactgga aggcgccgcg aaattaccat tggtggtaaa
     2401 atcataatca accccctgtg tggcggtggc cccaccgcca atactgaggg taactgtggc
     2461 agtgccaact ggcgctttat caatatgcat gttcagggta tagtccgtaa aatttctgca
     2521 gccactcgtt gatccggtag cttcagttct gagcggactg taattgtagg aagacgcgaa
     2581 acggatatag ggagtcgcgg gggggataac agaagaccat aatccacgcc cttgtgtgga
     2641 agccagaatg gtattatcac taaaacggta ttgaagcatg ttggtccgta cagttggaaa
     2701 actggaattc tgtacccaga cagtggatgc gccattcagc agactggttt catagattcc
     2761 catttcagta gccaggatcg ctttggtatt atcttccgga taaaacatgg cccatcgtac
     2821 aggaatatcc ggtaatccgc taccggtaat atccgtccag ccagctgcac cgcctccggt
     2881 agtactgagc cagacatgcg tggctccata gttcgaaaaa gtcgccagca aattattttc
     2941 cgtggtaccc tgcgccacac aactgacatt ggctacattc attgtactgc cagtgatatc
     3001 cgttgcggta aaagtagcac tatgcgcatt gtctacccgt acaatacgac cgttattggt
     3061 accaaaatac acccggttac tagtgtacct ggaaaccccg gtgtaactga cagttcctcc
     3121 actaaatgcg ggtaccgtgt ttgcaaaagc cgttgctccg ctgatcgcat taatccagcg
     3181 gatataggta cctgcattat acgcaccata aaaattatta ttgatgtcat cgtaatccgt
     3241 gggattgata aactgaccgg ctgaccctga atatgcaata ctgctccagc tggccccgct
     3301 gtttatactc cggtaatact ggttatatac ataggctcca aactggtact gcggctgatc
     3361 ttcatcaata tgaacatacg caccatcacc ccctgtaact tccacactgc tggtgatccc
     3421 tgcaccatta aactgatgta ccccattgtc ctgcgcgccg gccaggaaat aattagtggt
     3481 gcttggatgt atggcacagg cataaaattg tttcagtcga aggcccacat tccggtctgc
     3541 aaaagtgacc ccatcatctg cggaataaaa aagtccaccg tctcctccat ccagtacctg
     3601 gttattattc cagacaataa tctgatgatc ggcatgtata tagttggtgg agcccggaat
     3661 gccggttacc catactgaat ttacagacca ggtagccccg ccgtcggtac tgatataact
     3721 gttgagtccg ccaaccatca ctttgttcgc atcagtagga tctaccccaa tggcaataca
     3781 ataccaggcc tggccactgc tgaccggggc ggatcctgct atagggggag aagttccggt
     3841 aatagcccag ttatcaccgc catctatgga tttccagacc tggggtgtct ggaaagcgga
     3901 actggaagga agcgcataaa ccgtatttcc atttacggca atatcgcaat tatagcgtaa
     3961 agaagtaaaa gcggttgttg gggcagtcca ggttgcggaa gttactgtgg ccggattgtc
     4021 ggtatagcgg tagccggctg tcgcggcact ggtattataa taaccacagg ttatatgcat
     4081 tcttccggta ctgctgatca ccatttctga cacacgggtg gtcagccctg tcggacttaa
     4141 attagccgac caggtagccc caccgtccgt tgaccggtaa ataccacccg tactacccgg
     4201 aattcccacc aggctggcca ctgccgctat atataaatta ccggaggcat cacatacaat
     4261 tctggaacag ttccagaaag tggtggtgcc gggcaaaagt gaccaggtaa taccatgatc
     4321 tgtacttttc cagataccac caccggccac agcatcgata ttgatagctt tttcacctgt
     4381 agcaaaatac attacatccg tattggtcgg atcctgggtg atatcggcaa tcgcgaggtt
     4441 gcccaaaaag tcattggccg gggtccaatt aggagaggcc gccgtgatat ccgtggtttt
     4501 ccagacacct ccatccactc ccccaaccca tacggttttg tttgttacgt cagccagatc
     4561 cacccaaatg gcccgcatac ggccactggt tacgctggag ccaaggcggc cattgccatt
     4621 actgggtcca actgcatccg aactggatcc gcgttctgcc caactcaggg cattgagcct
     4681 gtttgtataa ttaccactgg cgcgtgccgc tttcaattgt tctgttgcct caatcagccg
     4741 gtttctgggt atatagccca gggttctgtc cgccgtgata tcaaactcca tacgctgcgc
     4801 gtcgctgata ccatcctggg ctttgggttt ttttacaaac ctgtcttttg ctgtaatttg
     4861 aatcgtattg ggttcggtac ccggattcat ttccatacat ccggaaagta aaagcaagag
     4921 gaatgggatt aacagaagtg gctggtaatt tctcatagat atctattgat ttatatttaa
     4981 aaatataaat aaaatcaagg aattggaaat cttttcacct tgacaatcag tacttgcgga
     5041 aagccgccgg tccgttgacg taaatctcct atatttgcac cccaaacagc tcttaagcat
     5101 gatcagcaga agaaatatcc gggttaaagt gatgcagacg ctctataccg tatcgcttgc
     5161 agaaaacgga gcaaaacccg gtgaagcgca gaaactctta caacaacatt ttgacgaaac
     5221 ccgctctttg ctggtatacc tcacctggtt ccttactgaa gtaacccgct acgccgaaac
     5281 tgatgctcat caacgggctt ccaaacacct cccctccgcc gatgatctga atgtgaatac
     5341 caaactggcc ggcaatgaat tattatggaa aatgctggaa gacggggaat taaaagaaca
     5401 gtttgcgaaa gaaaaaccgg aattgatggc caaccgggac ctgattcgca aaacctatat
     5461 tacactcgct gaaacaaccg attataaaac ctatatttcc aagggagccc gggacaggca
     5521 ggaagaaaag aaaatcctgg aatttatcct gaatgacctg atgctggcca acgaggtatt
     5581 tgtctcgcat attgaagaac atttttctaa ctgggacgat gatggggaaa tggtggtgca
     5641 actgctcagt ggttatctcc agaagccagg atccctgcat tttaaacaaa tgctgagtcc
     5701 ggataaggaa tccttcgcca aaacgctttt aaaaacagta caggataaaa atgagcatct
     5761 gcagaccctg attattccca aattgaaaaa ctgggatccg gaacggattg cccagctgga
     5821 tatgatcatg atgaaaatgg gcgtggcgga gttcctgtat tttgaaacca tccctcccaa
     5881 agtcaccatt aatgaatata tagacctggc caaggactat agcacccagc aaagtggtca
     5941 atttgtcaat ggcatactgg ataatattca taaagagctg gtccagcagg gaaaaatgga
     6001 aaaagtggac tataaaaaac cctgattcct gtccggccat tttatctaac tttgtcaacc
     6061 ttcataccac ttaaacaaat taagcagaca tgaaaaaaac agccctgatt gtgctggccg
     6121 ccctttccct tattgcctgt aaggaaatgg ataagaaagt ggccccggta gtaaagatgt
     6181 ccaaagaaga aattgaaaaa gcggtgacgg atacgactaa tttcaccacg attacctggc
     6241 tcgattccac cacgcaatca cttggtaaac ttaaaaaaga caaggaaatt gaaatcacct
     6301 ggcgttttaa gaataccggg gataaaaacc tgatcattga aaacgtaacg gcttcctgtg
     6361 gttgtaccat acccgaaaag ccggaacaac cttttgcacc cggacaggaa ggggtgatca
     6421 aagccaagtt taacggaagt ggcagtggca gcattaccaa gcaggtacat gtgattgcca
     6481 acacaaaacc aatgaaagat caaaccctta catttacagg ggttgatctt tcattggttt
     6541 tgtgttggca atc
//
LOCUS       DAOPMW010000167         6550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13457_length_6550_cov_5.120400, whole genome shotgun sequence.
ACCESSION   DAOPMW010000167 DAOPMW010000000
VERSION     DAOPMW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13457_length_6550_cov_5.120400"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLU11_08915"
     CDS             complement(<1..645)
                     /locus_tag="PLU11_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPN59281.1"
                     /translation="MKAVVFKKYGPPEVAQVMEIAKPFPKDHEVLIKVYSSTVNRTDC
                     GFRSAEYFVTRFWSGLLQPKMQVLGCEFAGVIEQIGGRVTSLKAGDRVFGFSDKKFGG
                     HAEYLTFPESGAIALMPDEIDFDEAAAITEGAHYALSDIRAAKVLPGQRVMVYGATGA
                     IGSAAVQLLKHFGAIVTAVCNTKNVELVKSLGADEVIDYMTQEFTWTKTRYDFIF"
     gene            complement(958..2190)
                     /locus_tag="PLU11_08920"
     CDS             complement(958..2190)
                     /locus_tag="PLU11_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59282.1"
                     /translation="MKRSLLSLLVLATLSTHGQTEFATYENGLIYSTATMTKLERIVD
                     SLNLKYKNCEMNKVYYSVPQTVGQLIELDSMDIKQAKRDMDNHISYDDFVKKYPSVKI
                     KKDVLVVKFRFKNYAGKDLVEYGEVNLDGHSGSRINKENVKDIYHQPMAGNWVYEYWE
                     GGQYTSESIKAFYFPQEFTTKIIVNKYARQIGYADCLVDTASTKFKKDLKAGWVDLPA
                     NWETLNAAAQKHLLDKMRGTKVVGGCSQDSRPREHAINIAILSAATTNWEVFLKAHLD
                     IMNDCFDRMSDGSYAQAGRKTYIRELEKLDINVTDLLIGISLRVENASDNHYYGSISR
                     LGRAISESANKAEFEQQMLDIIENTQLDDYNRVLGYYLFDSYNYNLESKPEQKKNDER
                     LKKSLQTLPVYLRNKIGV"
     gene            2440..3483
                     /locus_tag="PLU11_08925"
     CDS             2440..3483
                     /locus_tag="PLU11_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59283.1"
                     /translation="MHTLIRLFPALLLIIYGLSISQTFTSCKRPADKPNDTIVPPPPP
                     PLPPDTVTLIRRLEETYYQGNPGFTTRFRDYSFNYDAQKRLVSVGLKNHISFITDSLT
                     TRLFYTGNERYPRMIIKAYIYSQQPGPAMYGTTWITLDNQQRPIKDSSTDWSWNTTTN
                     VTEHVFPLVRHYSYNGQQAKIDWLARLGNGSTIDLIRKDSIWMSSDSLLQQSKTQYFN
                     GTIYPGNYTKAEAFISSQYLNPLALLNISGGLFSPIISPVRDEILGSNFHQSVYNSNP
                     LPYYIDFCNRKLPASFFLVGYNNNGYGMGSGGIYVSTLITPWSVRPSYPSEMKVSMSY
                     SYPDDKVFYKYFY"
     gene            3801..4124
                     /locus_tag="PLU11_08930"
     CDS             3801..4124
                     /locus_tag="PLU11_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPN59284.1"
                     /translation="MNLRRDVFQGIADPTRRAILLLVTTQAMTAGAIAANFDSARPTV
                     SKHLQILTECELLKQQPNGREIYYHLNLKKMKEVAGFIEPFRKMWEDRFVKLEQLMKV
                     YKPKK"
     gene            4136..4597
                     /locus_tag="PLU11_08935"
     CDS             4136..4597
                     /locus_tag="PLU11_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPN59285.1"
                     /translation="MKTEIIAEEGRQDLHIIREFDLPTELLYTAFIEPEIVEQWMGTK
                     VIKLDPARHGSYQFETTDPRGSVHRFSGTIHEVESNKKITRTFEMENTPFPVQLEFLS
                     FDPLPDHKSRLTMHIIYKSVTDRDNMLRLPFEQGINMAHNRLQEIFNNKKN"
     gene            4602..5180
                     /locus_tag="PLU11_08940"
     CDS             4602..5180
                     /locus_tag="PLU11_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPN59286.1"
                     /translation="MNPRVDQFLSKAKKWQIEMTLLRELILYCKLTEEIKWMHPCYTS
                     GQANIVLIHGFKDYCALLFFKGVLMKDPRNLLVQQTANVQTARQLRFSSLEEIRKLSP
                     QIKAYIKEAIRVENAGLKVEFKKTADYSIPEEFKTKLKQDKKLKAAFEQLTPGRQKGY
                     LLYFSSAKQTETRKRRIEKYIPEILKGKGIED"
     gene            5343..>6550
                     /locus_tag="PLU11_08945"
     CDS             5343..>6550
                     /locus_tag="PLU11_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPN59287.1"
                     /translation="MNLDFNRNEDQNKLLLSDLRQKLEKIHEGGGKKAADKQHEKKKL
                     TPRERISYLTDKGAPFIEIGAFAGYEMYAEQGGCPAGGTVAGVGYVSGRQCVILANDQ
                     TVKAGAWFPITGKKNLRLQEIAMENHLPVIYLVDSAGVFLPMQDEIFPDKEHFGRIFR
                     NNARMSGMGITQIAAVMGPCVAGGAYLPIMSDETLMVEGNGSIYLAGPYLVKAAIGED
                     IDNETLGGAETHTEISGIADYKFKTEEECMDHIKRMMGMLGEKPKAGFDRIAPKAPAK
                     DPNEVYGLYPTDGKNYNVVDIIERIADLSSEASAKEGESSFEQFKEDYGKTIVCGYAR
                     IEGWAVGIVANQRKIVKSRKGEMQMGGVIYNDSADKAARFILNCNQKKIPLVFLQDVT
                     GFMVGSRSEHS"
BASE COUNT         1913 a         1577 c         1373 g         1687 t
ORIGIN      
        1 aaatatgaaa tcataccggg tctttgtcca ggtaaactcc tgtgtcatat aatcaattac
       61 ctcatccgca ccaagcgatt ttaccagttc cacattcttt gtattacaaa cagcagttac
      121 gatggctccg aaatgtttta ataactgcac cgcagcagaa ccaattgcac cggtagcccc
      181 atataccata acccgctggc caggtaaaac cttcgctgcc ctgatatccg atagtgcata
      241 atgcgctcct tctgtaatgg cagccgcttc atcaaaatca atttcatcag gcataagcgc
      301 aatagctcct gattctggaa aggttaaata ttcagcatgg ccgccaaact tcttatcgct
      361 aaacccaaaa acacggtccc cggcttttaa tgatgttacc cttccgccaa tctgctcaat
      421 cacgccggca aattcacaac ccaatacctg cattttcggt tgcagcagac cactccagaa
      481 ccgggtaaca aaatactccg cgctacggaa accgcagtca gtccggttta ccgtggatga
      541 atagacttta attagtactt catgatcttt tggaaaaggc tttgcaatct ccattacctg
      601 ggctacttct ggtggcccat actttttaaa tacaactgct ttcataatcc cgatgtttga
      661 aagatgcttg attatgtcgt aaaaatgccc ctatttattg caatcatacg ttttagttat
      721 atcagtcact gttccggatg gttgtcctga aatgacggct gtagctacat gcccctgaag
      781 caggtctgca ttataagaga aacggcctca attaataaat gaatctgcaa tatagagaaa
      841 caggggacat taaaaaatga cctgggttag aactcatgag caatcctgag gttgattgtc
      901 accccggatt gaagttgtga ttcaactgat tgtataattt actgcttgta gctattgtta
      961 taccccgatc ttattcctca ggtataccgg caatgtctga agcgatttct tcagccgctc
     1021 atcgttcttc ttttgctccg gtttactttc caggttatag ttgtaactgt caaataaata
     1081 gtaccccagt acccggttat aatcatcgag ctgagtgttt tcaatgatgt ccagcatctg
     1141 ttgctcaaac tccgctttat tggcggactc gctgatggca cggcccagcc tgctgatgct
     1201 gccataataa tgattatcgg atgcattctc caccctcagg gaaataccga tcagcaggtc
     1261 ggtaacatta atatccagtt tttccagctc acggatatag gtcttccgcc cggcctgggc
     1321 ataactccca tccgacatgc ggtcaaagca gtcgttcata atatcgagat gagctttcag
     1381 aaaaacctcc caattggtag ttgcggcaga gaggatggca atattgattg catgttccct
     1441 tggccggctg tcctggctgc aaccacctac gaccttcgtc ccacgcattt tatccagcaa
     1501 atgcttttgt gctgccgcat tcagggtttc ccaattcgct ggcaaatcca cccaacccgc
     1561 cttcaggtcc tttttaaatt tagtacttgc tgtatccacc aggcaatctg cataaccgat
     1621 ctgccgggca tatttattca caataatttt agtggtaaat tcctgtggaa agtaaaaagc
     1681 cttgatggac tccgacgtat actgcccacc ttcccagtac tcatacaccc agtttcctgc
     1741 cattggttga tggtatatat cttttacatt ctctttgtta atccgggaac ctgagtggcc
     1801 atccagattg acttcaccat actccaccag gtccttaccc gcataattct taaacctgaa
     1861 tttgacaacc agtacatcct ttttaatttt cactgacggg tattttttca caaaatcatc
     1921 ataagaaata tggttatcca tatccctttt tgcctgctta atatccatgc tatccagttc
     1981 aatcaattgt cccaccgtct gtggtactga atagtaaacc ttattcattt cacaattctt
     2041 atatttcagg ttcagagaat caacaatccg ttccagcttt gtcatagtgg ctgtactgta
     2101 aatcagcccg ttttcatagg ttgcaaattc tgtttgacca tgtgtggaca gggtagccag
     2161 taccaggaga gaaagcagcg atcttttcat cttgatggtt tttaatatta agcaatacct
     2221 gtgttcaagg aatggattcc tgtaagacag gcaaatacgc ctggaaaaga ttgaatacag
     2281 taatttgtac taaatatttt cccggttgta tgatgtagat gcaggggatt gttaaattac
     2341 ataaaaaatc tgcgggtgac ctctagttta aacagcctgc tttccttggt ttgcgggaat
     2401 cagataattt aaatatttta cagcgcaatt cagctatcta tgcacaccct tatcagatta
     2461 ttcccggctt tacttttgat tatatacggc ctgagcattt cacagacatt caccagttgt
     2521 aagcgtcccg cagataaacc caatgatacc attgtaccac caccccctcc gccgttgcca
     2581 ccggacaccg tgacgctgat ccgtagactg gaagagacct attaccaggg aaacccgggt
     2641 tttaccacaa gattcaggga ttacagtttt aattatgacg cccagaaaag actggtgagt
     2701 gttggactaa aaaaccatat ctccttcatt actgacagcc tcaccacccg tttattctat
     2761 accggtaatg aacgttatcc ccggatgatt atcaaggcct acatttacag tcagcagccc
     2821 ggtccggcca tgtatggcac tacctggatc accctggaca accagcaacg ccccataaag
     2881 gattccagta cagattggtc ctggaatacg accacaaatg ttacagaaca tgtatttccc
     2941 cttgtccgcc attatagtta caacgggcaa caagcaaaga tcgactggct ggcccggctc
     3001 ggcaacggca gtaccatcga cctgatccgc aaagattcca tctggatgag cagcgacagt
     3061 ctgctgcagc aatccaaaac ccagtatttc aacggtacta tctacccggg aaattataca
     3121 aaagcagaag cttttatttc ttcccagtac ctcaacccgc tggctttact gaatatcagc
     3181 ggcggcctct tctctcccat tatctctccg gtccgggatg aaatactagg cagtaatttt
     3241 caccagtcag tctataactc caatccctta ccctattata tagatttctg taaccggaaa
     3301 cttcctgcca gtttcttcct ggtcggctat aataacaacg gctatggcat gggaagcggc
     3361 ggtatctatg tcagcacctt aataacgcca tggtcagtaa gaccatctta tccctcggaa
     3421 atgaaagtgt ccatgagcta ctcatatccg gacgacaaag ttttctataa atacttttat
     3481 tagaaagaag agagccgaaa tagtatctcg tgagtaatac ggaaagagta gaggattgag
     3541 cgttgagaga atcttataga agaaagatgt tgaaagatgg gtttatatgt ttagtcgttt
     3601 ggccttgaga aggacattac tcctatcacg ccctcttttt cctctattat ccgccgaagc
     3661 taatgcgaag gagatctttg accactttag cgccccctgc agagccaatc ccacatcata
     3721 ccccctcacg acccttccct cccaaatata tttgcgtagt tgaatgactt catatatatt
     3781 tgtagccaaa tgacttcata atgaatttac gacgtgacgt tttccagggg attgcggacc
     3841 caacaagacg ggcgatccta ttgctggtaa ctacccaggc aatgaccgct ggtgctatcg
     3901 ccgctaactt tgacagtgcc cggccaaccg tctccaaaca tcttcagata ctgaccgaat
     3961 gtgaactgtt gaaacaacag ccaaacggca gggaaatcta ttatcatttg aacctgaaaa
     4021 aaatgaaaga ggtggctggg tttattgagc ccttccggaa aatgtgggag gacaggtttg
     4081 ttaagctgga acagttgatg aaagtctata aaccaaaaaa ataataagga cggaaatgaa
     4141 aacagaaatc atagctgaag aaggcaggca ggacctgcat ataattcgcg aatttgatct
     4201 ccccacagaa ctgctgtata ccgcatttat tgaacccgaa atagtggaac aatggatggg
     4261 gacaaaagtg ataaaactgg accctgcccg gcacggcagt taccaatttg aaaccaccga
     4321 tccccgtggc agtgtacacc gtttcagtgg cacaattcat gaagttgaat ccaataaaaa
     4381 aatcacccgg acgtttgaaa tggaaaatac gccctttcct gtccagcttg aattcctttc
     4441 cttcgatccg ctgcccgatc ataaaagcag actgaccatg cacattatat ataaatcagt
     4501 aactgaccgg gacaatatgc tccgcctgcc ttttgagcag ggtatcaata tggcacataa
     4561 ccggctccag gaaatattca acaacaaaaa aaactaaaca tatgaatccc cgagttgatc
     4621 agtttctgag caaagcaaaa aaatggcaga ttgaaatgac gctgctaaga gaactgatcc
     4681 tatactgtaa actaacggaa gagatcaaat ggatgcatcc ctgctataca tccggacagg
     4741 cgaatattgt cctgatccat ggatttaaag attactgcgc cttgttattc tttaaaggag
     4801 tattgatgaa agatccgagg aacctgctgg tgcagcagac cgcaaacgta cagaccgcaa
     4861 ggcaactccg tttcagcagc ctggaagaaa tcaggaagct cagcccgcag ataaaagcct
     4921 atatcaaaga agccatccgc gttgaaaatg cggggttaaa ggtggaattc aaaaaaacag
     4981 cagactacag cataccagaa gaatttaaga cgaaacttaa acaggataaa aaactaaaag
     5041 ccgcttttga acaactgaca ccgggcagac aaaaagggta cttactttat ttctcttctg
     5101 ccaaacaaac agaaacccgt aaacgaagga ttgaaaagta tatcccggaa attctgaagg
     5161 gaaaaggtat tgaggactga gatgaggtga actacgggaa gtttatccgt ttatacctgc
     5221 ttgaattgtt ttacaccatt tccggaactt aaatccccgc ttacgccgca caaatgaata
     5281 acacccagca actatcagct aacacctaac aaccttatct ttgccctcta acgactcctc
     5341 ccatgaacct cgactttaac cgaaacgaag accagaataa actcctcctc tccgatctcc
     5401 gtcagaagct ggaaaaaatc catgaaggcg ggggtaaaaa agccgcggat aaacaacatg
     5461 aaaaaaagaa actcaccccc cgggaaagga tttcttacct gacagacaag ggggctccct
     5521 ttatcgaaat aggggctttt gccggctatg aaatgtatgc cgaacaagga ggttgtccgg
     5581 ctggcggtac cgtggccggg gtgggctatg tcagcggcag gcaatgtgta atcctggcca
     5641 atgaccagac cgtcaaagcc ggggcctggt ttcccatcac aggaaaaaag aacctgcggc
     5701 tgcaggagat cgcgatggag aatcatttgc ccgtgatcta tctagtggat agtgccggtg
     5761 tcttccttcc catgcaggat gagatttttc cggataaaga acattttggc cgcatcttcc
     5821 gaaacaatgc acgcatgagc ggcatgggta tcacccagat cgcagccgta atggggccct
     5881 gtgtggccgg tggcgcctac ctccccatca tgagcgacga aaccctgatg gtggaaggca
     5941 acggatccat ttacctggca ggaccttatt tagtcaaagc cgctatcggc gaagatattg
     6001 ataacgaaac cctgggaggt gcagaaacac atacggagat cagtggtatt gcggattaca
     6061 aattcaagac cgaagaggaa tgcatggacc atatcaaacg gatgatgggc atgctgggcg
     6121 aaaaaccaaa agcaggcttt gaccggattg ctcctaaagc cccggccaaa gacccgaatg
     6181 aagtatatgg cctctatccc accgatggaa aaaattataa tgtggtggat attattgaac
     6241 ggatagccga cttgtcctca gaagcatcag cgaaggaggg cgaaagttcc ttcgaacagt
     6301 tcaaagaaga ttatggcaaa acgatcgttt gcggttatgc aagaatcgaa ggctgggccg
     6361 tcggtattgt agccaatcaa cgtaaaattg tgaagagccg taaaggagaa atgcagatgg
     6421 gcggtgtgat ttataatgat tcggccgata aagcagcccg ctttattctc aactgcaacc
     6481 agaaaaaaat tcccctggtt ttcctgcagg atgtcaccgg cttcatggtc ggcagccgca
     6541 gtgaacattc
//
LOCUS       DAOPMW010000168         6528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13513_length_6528_cov_10.451259, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000168 DAOPMW010000000
VERSION     DAOPMW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13513_length_6528_cov_10.451259"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1336
                     /locus_tag="PLU11_08950"
     CDS             35..1336
                     /locus_tag="PLU11_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPN59288.1"
                     /translation="MTLSEDQILALAPDEASKKAGLGLANMSKWVSKGANELAIWGEC
                     QGSGSKPYQAQIDLSNIAFKCSCPSRKFPCKHGLALFLLYARQTASFSIDKAPAWVTE
                     WLEKRAEKQEKKAAKEEAPVDEAAQVKRQLARHDKVMNGINELQLWIKDIVRNGILSM
                     PDKGFPWFENVAKRMVDAQAPGLANMVRQLGETNFFAENWQQPFLEQLLKLYIVTEGY
                     QQHHSGEAPFLQDLRSWIGFTINQDELKEQTGITDTWLVLAKQSNEVDNVTTEKYWLY
                     GLEQKKYALVLQFTVRGQGSQLSLTPGLYIQAELVFFPSVNPMRALIKRQLNTQNNNI
                     PTGYSGWDEVTAAQSEMYSRFPVQGERPFIVEQLTAVPYNNQWWLRDQSSRMVPIRNA
                     EKNIWHILAISGGQPLNMVLIGNPAQVEVAGIWQEHQYLLV"
     gene            1346..2833
                     /locus_tag="PLU11_08955"
     CDS             1346..2833
                     /locus_tag="PLU11_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HPN59289.1"
                     /translation="MQSWEDVIHTAMIGTDKKILSVSQLPESLAAVQTMLTDKSGNDK
                     EILYLQQSSLLMNYKQCGLSLLQKEEIGTSMAQPEEKPYCSAVAAQVLKEVLNAEAPL
                     LLEYWLRRCAENEQIFPPVFIPALFSKARQEKYLQGLVVRCSGKRGEWMCGLNENWNF
                     YRGENTEETWQTGTQEQRRTVLKETRRTNPALGLTWLQQTWATEDAAGKLAFLEVLKT
                     GLNEGDISFLESLHTEKSKKVKEEAVNLLKHTPGSSLIRLYQQVLAEAVQVKKERSLM
                     GLSSKTVLQFKLPSSVPEAIFESGIQKMSSQKNISDDRFIIYQLISFTPPVFWEQHFQ
                     LKPVEIIDLFCKTEEGYEHTGAIGMAASRFPSPEWAALMIEDENRLFLDLIVQLSPEQ
                     KEKYLLKHFEKAGDTLIQLVGNTTVEWSVSLTKAIFKYTSTNPNQFTKTYYRDHIHLI
                     PVGVVPELTRCTPSESYPAVAWGNTCDYLRELLSLKEQIHQSFNS"
     gene            2845..3927
                     /locus_tag="PLU11_08960"
     CDS             2845..3927
                     /locus_tag="PLU11_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN59290.1"
                     /translation="MASILRQHAEQLFGAELEELKKQDTDKRPDNWLMSPRSVVQYLL
                     GGKLKNGFEVTPKYIGNKRLMEVAVATLTTDRALLLYGLPGTAKSWVSEHIAAAISGD
                     STLIVQGTAGTGEESIRYGWNYARLLAEGPSERALVETPVMRAMKDGKIARIEELTRI
                     GADVQDALITILSEKTLPVPELNTEIQSIRGFNIIATANNRDKGVNELSSALKRRFNT
                     VILPVPDSIEEEIDIVRRRVESFGKVMDLPAEPPALQEIKRIVAIFRELRNGVSADGK
                     TKIKSPSGTLSTAEAISVVNNGMAMAAYFGDGRLKAHDLAAGIIGAVVKDPVQDRLVW
                     QEYLETIVKPRDEWKDIYRACRDQGF"
     gene            3938..6139
                     /locus_tag="PLU11_08965"
     CDS             3938..6139
                     /locus_tag="PLU11_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HPN59291.1"
                     /translation="MAVHVLGIRHHGPGSARNVKDFLEQLKPDIVLVEGPPEADDILP
                     WSIHPELKPPVAILCYQPGNPQQSSFYPFAEFSPEWQAIRYARQQNIHVRFMDLPAAH
                     QFALEEEKKNAPEDESEAHILHRDPISWLGRAAGFDDGEKWWEHMFEHRQNSESVFDA
                     VAEAIQALRDELPPKEDRLEQTREAHMRKMIRQAEREMFQQIAVICGAWHVPALVNMP
                     SVKEDTETLKGLPKVKVETTWVPWTYRKLSFFSGYGAGIQSPGWYEHVWKYPADDGTR
                     WMTKVAGLFRRQQMDTSVAHVIEGVRLANALAALRNLSKAGLEELNEATQSILCNGEG
                     IKMSLVGDELIVSNKMGAVPEDIPRPPLQVDIEKQQKKLRLPPTADWKDYTLDLRKEN
                     DLDRSIFLHRLRLLGIRWGEQSDAKSKGTFKEQWRLQWEPSFTIEMIEKGSWGNTTEE
                     AASAFTIDQVQKASNLVAVCDLLQELIPAELEKAIDTCISQINNLAAASADVMELLQV
                     IPGLVSVSRYGNVRKTDAGLITGILQSMITRVCVSLPSACVGVDEDAATLLVELFRKM
                     NDAVGILQLEEITGEWQNTLTHIARNKNTVPLLAGYTTRLLSDYKLLEGEELVKLFSY
                     SMSFAGQPAVAAAWLEGFLKGSGTILLIDEDLWSLVHNWVSELDEENFVQLLPLLRRT
                     FAHYSAAERRKLGEKVRQGGGPVNRPVLAGIDEERAKQGIEIILNLFGHKP"
     gene            6144..>6528
                     /locus_tag="PLU11_08970"
     CDS             6144..>6528
                     /locus_tag="PLU11_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59292.1"
                     /translation="MATETELRKWRLILGGQQNDGTGCALNISDIKMDHALEALYDSD
                     RRGGLGASSPNVSRWLGDIRNFFPNTVVQVMQKDAIKRLDLTQLLFEKELLENVEADV
                     QLVATLLSLKNVIPDKTKDTARQVVQ"
BASE COUNT         1898 a         1459 c         1666 g         1505 t
ORIGIN      
        1 acttatccaa tagtttagca ggtaaagatt tccaatgacc ctgtccgaag atcagatact
       61 ggcgctcgca cccgatgaag cttctaaaaa agcaggcctt gggttggcga acatgtccaa
      121 atgggtcagt aaaggcgcca atgaactggc tatctggggt gaatgccagg gaagtggcag
      181 caaaccttac caggcacaaa ttgatctctc caacattgcc ttcaaatgtt cctgtcccag
      241 tcgcaaattt ccctgcaaac acgggctcgc cctttttctc ctttatgccc ggcaaaccgc
      301 ttccttcagt attgacaaag cccccgcctg ggttacagaa tggctggaaa aaagagcaga
      361 aaaacaggaa aagaaagcgg caaaggaaga agcaccagta gacgaagccg cccaggttaa
      421 gcgccagctg gcccgccatg ataaagtgat gaatggaata aacgaactgc aattatggat
      481 aaaagacatt gtccgtaatg gcattttgtc catgccggat aaaggatttc cctggtttga
      541 aaatgtggcg aaaaggatgg tggatgcaca ggctcccggt ctggcgaata tggtccggca
      601 attgggagaa acaaatttct ttgctgaaaa ctggcaacag ccatttctcg aacaattact
      661 gaagctctat attgtaactg aagggtatca gcaacatcat tccggagaag ccccgttttt
      721 acaggatctg cgcagctgga tcggttttac cattaaccag gacgaattaa aagaacagac
      781 cggcatcaca gatacctggc tcgtgttggc caaacagagt aatgaagtgg ataatgtaac
      841 tacggagaaa tactggctct atggtttgga gcagaaaaaa tacgccttgg tactgcagtt
      901 caccgttcgg ggacaaggca gtcagttatc ccttactccc ggactttaca tacaggccga
      961 actggttttc tttccttcag tgaacccgat gcgtgccctg atcaaacgcc aattgaacac
     1021 acaaaataat aatataccaa ccggttactc cggatgggat gaagtaacag cggctcaatc
     1081 agaaatgtat agccggtttc cggtacaggg agaacgaccc tttattgttg aacaactgac
     1141 agctgtccct tataataatc aatggtggct gcgcgaccag tcatcgcgga tggttccgat
     1201 acgtaatgcc gagaaaaata tctggcatat actggccatc agcggaggac aaccgttaaa
     1261 tatggtgctc attggcaatc cggcccaggt ggaagtggcc ggcatctggc aggaacatca
     1321 atatttgttg gtttaaatta atagcatgca aagctgggaa gacgtcatac atacagccat
     1381 gatcggaacg gataaaaaaa tcctttctgt cagccaactg ccggaaagcc tggctgcagt
     1441 gcagacaatg ctgaccgata aatccggaaa tgataaagaa atcctctatc tgcaacagtc
     1501 ttccctgctg atgaattata aacaatgcgg cctgtccctt ctgcaaaaag aagagattgg
     1561 caccagtatg gctcagccag aagaaaaacc ttactgcagt gccgttgccg cacaggtatt
     1621 aaaagaggtg ttgaatgcgg aggctccgct gctgctggag tactggcttc gtcgctgcgc
     1681 tgaaaatgaa caaattttcc ctcctgtttt tattccggcc cttttctcaa aagccaggca
     1741 ggagaagtat ctgcagggat tagtggtacg ctgcagtgga aaaagaggag aatggatgtg
     1801 cgggctgaat gaaaactgga atttttaccg gggtgaaaac acggaagaaa cctggcagac
     1861 tggcacgcag gaacaacgtc gtaccgtttt aaaagaaacc aggcgcacta atcccgctct
     1921 tggattaacc tggctgcagc agacctgggc cacagaagat gccgcaggca aactggcttt
     1981 tcttgaagtc ttgaaaaccg gattaaatga aggggatatc tcttttctcg aatcattaca
     2041 tacggaaaaa agtaaaaaag taaaagaaga agcggtcaac ctgctcaaac atactcccgg
     2101 ctcttcactg atcaggcttt accagcaggt actggccgaa gccgtacaag taaaaaaaga
     2161 aagaagcctg atgggcttat ccagtaaaac agtcctgcag ttcaaactgc cttcctccgt
     2221 gcctgaggcc atttttgaat ccggtattca aaaaatgagc tcgcaaaaaa acatcagtga
     2281 tgaccggttt attatttatc aactgatctc ctttacacca ccggtattct gggaacagca
     2341 ttttcagttg aaacccgtgg agatcattga tcttttctgc aaaactgaag aaggatatga
     2401 acatacgggc gccatcggca tggccgccag ccgttttccc agtccggaat gggccgcact
     2461 gatgatcgaa gatgaaaatc gtctcttttt agacctcatt gtccagctat cacctgaaca
     2521 gaaagaaaaa tacctgctga aacattttga aaaagctggt gatacgttga ttcagctggt
     2581 cggcaataca acggtggaat ggagtgtgtc attgacaaaa gccattttta agtacaccag
     2641 cactaacccc aatcagttca caaagaccta ttaccgggat catattcatt taattcctgt
     2701 gggggtggtt ccggaactca cccgttgcac cccatccgaa tcctatccgg cagttgcatg
     2761 gggcaatacc tgtgattacc tgcgggaatt attatccctc aaagaacaaa tccatcaatc
     2821 attcaactca taaaacgacg ctgtatggca agcattctgc ggcaacatgc cgaacaatta
     2881 ttcggagcgg aactggaaga actgaaaaaa caggatacgg ataaaagacc cgacaactgg
     2941 cttatgtcgc cccgttcagt ggtgcagtac ctgctgggag ggaaactgaa aaacggtttc
     3001 gaagtaactc cgaagtatat cggcaataaa cggttgatgg aagtagccgt ggctacttta
     3061 accacggacc gggctttatt attatacgga ttacctggta cagccaaaag ctgggtgagt
     3121 gagcatattg cggcagcgat cagtggtgat tccaccctga tcgtacaagg taccgcgggc
     3181 accggcgaag aatccatccg ctatggatgg aactatgcgc ggctgctggc tgaaggacct
     3241 tctgaaagag cattagtgga aacacccgtg atgcgggcca tgaaagatgg caagattgcc
     3301 cggattgaag agctgacccg gatcggtgcc gatgtacagg atgcactgat cactattcta
     3361 tctgaaaaaa cattgcccgt acctgaactg aatacagaga ttcaatctat acgtggtttc
     3421 aatataattg caactgcgaa taatcgtgat aaaggcgtga atgaattgag cagcgccttg
     3481 aaaagacgat ttaataccgt tatccttcct gttccggatt ctattgaaga ggaaattgat
     3541 attgtcaggc gccgggtcga aagttttggt aaagtgatgg acttaccagc tgaaccgccc
     3601 gcacttcagg agatcaaaag aatagttgcc atattccgtg aattacgcaa tggagtgtct
     3661 gccgatggca agaccaagat caaatctccc agcggtacat tgagcaccgc tgaagccatt
     3721 tcggttgtca ataatggaat ggccatggct gcttattttg gggatggccg cctgaaagcc
     3781 catgacctgg cagcaggtat catcggtgcc gtagtgaaag acccggtgca ggaccggctg
     3841 gtgtggcagg aatacctgga gaccattgta aaaccaaggg atgaatggaa agatatatat
     3901 cgtgcctgcc gtgaccaggg cttttaaata aaacaggatg gcagtacatg tattaggtat
     3961 acgacatcat ggccccggct ctgccaggaa cgtaaaagat ttcctggagc agctgaagcc
     4021 ggatattgta ctggtggaag gtccaccgga agcggatgat atactgccat ggagtattca
     4081 cccggaatta aaaccgccgg tagctattct ctgttaccag cccggcaatc cgcaacaatc
     4141 ttccttctat ccgtttgccg aattctcccc tgagtggcag gccatccgct atgcccgcca
     4201 gcaaaatatt catgtccgct ttatggacct gccggccgct catcagtttg ccctggaaga
     4261 agaaaagaaa aatgcgccgg aggatgaatc agaagcgcat atacttcacc gtgatccgat
     4321 cagctggctg ggccgggcgg ccggatttga cgatggagaa aaatggtggg aacatatgtt
     4381 tgagcaccgg caaaacagtg aatccgtttt tgatgctgtt gccgaagcga tacaagccct
     4441 ccgggatgaa ttacctccta aagaagaccg gcttgaacag acccgcgaag cgcatatgcg
     4501 taaaatgatc cggcaggccg aacgggaaat gtttcagcag attgctgtga tctgtggcgc
     4561 ctggcatgta ccggccctgg tgaatatgcc ttccgtaaaa gaagatacag agacattgaa
     4621 aggattaccc aaagtaaaag tggaaaccac ctgggttccc tggacctacc gcaaactgag
     4681 tttcttcagt ggttatggcg ccggtatcca atcacccggc tggtacgaac atgtatggaa
     4741 atacccggct gatgacggta cacgctggat gaccaaagtg gctggactct tccgcaggca
     4801 acaaatggat acctcagtag cgcatgtaat tgaaggggta agactggcta atgcacttgc
     4861 tgctttacgg aatttatcga aagcagggct ggaagaactc aatgaagcca ctcagagtat
     4921 cctctgtaat ggtgaaggaa ttaaaatgag cctggtgggt gatgaactga tcgtaagtaa
     4981 taaaatgggg gccgtgccgg aggatattcc aaggccgcca ttacaggtgg atattgaaaa
     5041 gcaacaaaag aaattgcggt taccacccac tgctgactgg aaggattata cgcttgacct
     5101 gcgtaaggaa aatgatttag atagaagtat ctttttgcac cggctccgct tactcggtat
     5161 tcgttggggc gaacaatccg atgctaaaag taaaggcacg tttaaagaac aatggcggtt
     5221 gcaatgggag ccttctttta ctattgaaat gatagaaaaa gggagttggg gtaacacgac
     5281 ggaagaagcg gcgtctgcct ttaccattga ccaggtacaa aaagcaagta acctggtggc
     5341 cgtatgcgat ctcttgcagg agctgatacc agctgaactt gaaaaagcca tagacacctg
     5401 tatcagccag atcaataacc tggccgcagc cagtgccgat gtcatggaac tgttgcaagt
     5461 aatacccgga ctggtatctg taagccgcta tggtaatgta cgtaaaacgg atgcaggcct
     5521 gatcaccggc attctgcaaa gtatgatcac ccgggtctgt gtgagtctgc cctctgcctg
     5581 cgtgggtgtg gatgaagatg ccgcgacact tcttgtggag ttgttccgga aaatgaatga
     5641 tgccgtgggt atacttcagc tggaagagat taccggtgaa tggcaaaaca cccttaccca
     5701 tattgcccga aataaaaaca cggtgcccct gctggcgggt tacactacca ggcttttatc
     5761 cgactataaa ttactggagg gtgaagaact ggtgaaatta ttttcctatt ccatgagttt
     5821 tgccggtcag ccggcggtgg cagcggcctg gctggaaggg ttcctgaaag gcagcggtac
     5881 catcctgtta atcgatgaag atctctggtc actggtacat aactgggtga gtgaactgga
     5941 tgaagaaaat tttgtacaac tgcttccgtt gttgcgccgg acattcgctc attattcagc
     6001 tgctgaaaga agaaaactcg gtgaaaaggt aagacaggga ggcggaccgg tgaatcgtcc
     6061 ggtgctggca ggtatagatg aagaaagagc gaaacaagga attgaaatta tattaaatct
     6121 tttcggacat aaaccctgaa ggaatggcca cagaaacaga actacgaaaa tggcggttga
     6181 tactgggcgg tcagcagaat gacggtaccg gttgtgcact gaacatatcc gatattaaaa
     6241 tggatcatgc actggaggcc ttgtatgaca gtgaccgccg gggtggatta ggtgcctcct
     6301 ctcccaatgt aagccggtgg ctgggggata tccggaattt ttttcccaat accgtggtgc
     6361 aggtgatgca gaaagatgcg atcaaacgac tggacctgac gcaattgctg tttgaaaaag
     6421 agttactgga aaatgtggaa gcggatgtac aattagtggc caccttactc agcctgaaaa
     6481 atgtaatacc cgataaaaca aaagatacgg cccggcaggt ggtacaga
//
LOCUS       DAOPMW010000169         6460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13750_length_6460_cov_9.587041, whole genome shotgun sequence.
ACCESSION   DAOPMW010000169 DAOPMW010000000
VERSION     DAOPMW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13750_length_6460_cov_9.587041"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLU11_08975"
     CDS             complement(<1..846)
                     /locus_tag="PLU11_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN59293.1"
                     /translation="MKTLLIKSTFFALLVLSLASCKKWIDYNPQEEFKITELDYLQSE
                     SDYRTMAVSVYTPLQWLNQAVVVGEIASDNAVSGGENASDVLPLQQIDDYTHTPNNAT
                     LSELWKSAYEGVNRANYLTQYKAANPAGKSVEFAGKEALYGEIHFLRAYYYFTLVKFF
                     GDVPLFTDKRLGLAESGSLGRSPKADVYKQVENDLIAAIAVLPAVQVQKGRITKYAAQ
                     ALLGKVYLYQKKYNEAAPVLESIITANAFTLVNDYAGMYLSTGENGTESVFEIQYSNT
                     SPYYNW"
     gene            complement(869..3994)
                     /locus_tag="PLU11_08980"
     CDS             complement(869..3994)
                     /locus_tag="PLU11_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN59294.1"
                     /translation="MKLKTALLSLCVLLTGLLQMASGQNLPVSGTVSNKSTGEPLVGA
                     TISVKGTRTNTMTDAKGQFTIAIPKAGSVLVFSYTGMKQVEFTVSDNKSAVNIQLEES
                     TTSTLTDVVVVGYGTQKVTKVSGAISTIKSADIEKLKPVRVEEALQGRASGVVVVNSG
                     TPGAKPTVLVRGIPSFSGTDPLVIIDGVPQSLTDFNSINAADIESINLLKDAATTAIY
                     GVKGGNGVIVVTTKNGRKNQKTDITFNSNYGVQEVVNTLGVLNASEYAAIINEGSTTA
                     GGQVIFPNLSTVGVGTNWQNEVFKMASMQSHNVSARGGSEKMSYFLSGGWLSQGGIVG
                     GNDKSRFNRGNLTANLNFDLAPRLKFILNTTAVLLDGRGIQENSFNSVLGSAINFDPT
                     VPKYNTVPNTVGTYGYSNLLLSEIFNPLTKLDNTFNKNVGNKLYGKGELQYDVLKNLK
                     LTSRFGYTKYDGNAKSFSPLVFYGPLNVENSMNADGSTVTGRFNSVSHEKTSNFNWTW
                     ESFANYNFKVKEDHNFETVLGFSMAKTSGNAAGASRQDVPFNSWEFADFTAATGNNTA
                     TNTNAVTGYYYQYFRRNLSYFSRINYDFQEKYLASFTARRDGSYAFGKNNKYANFFSG
                     SLGWVVSKENFFKSEFIDHLKIRGSYGSIGNENVDPQFVSIVTGGPSYGPTANSNGYT
                     FGDIFYPGSTVGSAANDALAWEKQLQANVGFDMTFYKRRFTISADYFQKEVEGLLFTP
                     SGSLYLGTVPFPTANIGSTKSSGIDLMLSFNETFGKSFKFNTSVTFTTSKNEVTATNN
                     DGTAKILGGFYFNGQSQNVTVFEKGQTPGYFYGFKTNGLFQNYTEVNAGPTQTGAQPG
                     DIRFVDMNKDGVINDADKTKIGDPFPEFTLGWNVNLEFKNFDFTAFTYASVGNEVYRA
                     YERNANYTNKDRMILARWTGENSTNSAKNPRYSFTDANSNIRVSDRYVEDGSFIKIKN
                     LQLGYTFPQQMTRKVFSRLRIYAQVKNALTFTKYTGFDPEIAGGIMDTGVDRGAYPLA
                     RTYAVGIDIKL"
     gene            4191..>6460
                     /locus_tag="PLU11_08985"
     CDS             4191..>6460
                     /locus_tag="PLU11_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN59295.1"
                     /translation="MRKFILLVFLLPVTVFCQNTIGLPDIINYSKQAYAAGLQNWDIR
                     QDRQGILYLANNEGVLSFDGKYWNLYPLPNKTIVRSVETGAGDRVYAGGQDELGYFSP
                     GRNGSLRFTSLVELLPEKERSFGDVWDICVYKKDVYFRTPGKIFRYTGNAMAVFTAPS
                     EWSYLGVCGDRLFAHDYVNGIMTFNNNIWSPVQTSNQLPGSDPITAILPIGRDSSLVT
                     TLKNGIYILSKEGAVKLNSPALALIEKDRIYAATAINAEWVALATNNNGVHIVDTRGN
                     LIQQFSRSEGLQNNNVLSIFLDRQQNLWLGLDNGIDFIAYNSAIKRISPYSQDGSGYS
                     AIIHKDVLYTGTSAGLYSVPLQPLQDYSFSKGQFTQVTNTTGQVWGLATINNTLLLGH
                     HEGAFIVDGNTATPFFSSEKGVWNFIPLSTVFPTARMITGTYRGLSLFDYSNNRFIPA
                     ENVKGLDESCRFVAIDKDNAVWVSHPYHGVYRLNPDGNGQYQSKLYTAANGLPSTLNN
                     HVYKINNEVVVATEKGVYGFDPAKDRFIPSPYYQELLGQQSIRYLKEDPEGNIWFIHD
                     KQLGVLDRRDKKPQLIHLPELSSKMLSGFEFIYPVNNNNVFIGAEKGFFLINYEKYKK
                     NIPVLEVMVRNVRIFNRRDSLLYGGYAADSGKSAQPSIAHQWGTLHFEYSSPLFGQQT
                     SLEYSCRLRGFDDNWSEWSAKTEKEYTHLPAGSYHFEVKVRNNLGNESAPHIYSFRIL
                     PPWYQTLWAYLVYFLIG"
BASE COUNT         1660 a         1526 c         1555 g         1719 t
ORIGIN      
        1 ccagttataa taaggggagg tgttggagta ctggatctca aaaacggatt cagtaccatt
       61 ttcaccggtt gacaggtaca tacctgcata atcattcacc agtgtaaacg cattggctgt
      121 aatgatgctt tccagcacgg gagcagcttc attgtatttt ttctgataga gatatacttt
      181 accaaggagt gcctgtgcgg catacttggt gatccgtcct ttctgtacct gtacggcagg
      241 taaaacagca atggcggcaa tcaggtcatt ttccacctgc ttgtatacat ctgctttagg
      301 actacgaccc aggctgccgg attcagcaag acccagacgc ttgtcggtaa acagcggtac
      361 atcaccaaag aatttcacga gggtgaaata ataataagca cggaggaaat gaatttctcc
      421 ataaagggct tccttaccgg caaactctac tgacttaccg gccgggttag cggctttata
      481 ctgggtcagg taattggcgc ggttcacccc ttcataagct gacttccata attctgataa
      541 agtcgcgtta ttgggcgtat gcgtgtaatc atcaatttgc tgcaggggta acacgtcaga
      601 agcgttttca ccaccactca ctgcattatc cgatgcaatc tcaccgacaa ccacggcctg
      661 gttcagccat tggagcgggg tatacacact cactgccatg gtgcggtagt cgctttcaga
      721 ctgcaggtag tccagttcag taatcttgaa ctcctcctgg ggattgtagt cgatccattt
      781 tttgcaggag gccagtgaca ggaccagcag tgcaaagaag gttgatttaa ttaataaggt
      841 cttcattgta taataatttt ataacggttt aaagtttgat atcaataccg acagcatagg
      901 tacgtgccag gggatatgct cctctgtcta cgcctgtatc catgataccg ccggcgatct
      961 caggatcaaa tccggtatac ttggtaaatg tgagtgcgtt tttcacctgt gcataaatac
     1021 ggaggcggct gaagaccttg cgggtcattt gctgcgggaa ggtgtaaccc agctgcaggt
     1081 ttttgatttt aataaaagaa ccatcttcca cataccggtc agatacgcgg atattgctat
     1141 tcgcatccgt aaaggaataa cggggattct tagcgctgtt ggtgctgttc tcaccggtcc
     1201 aacgggccag gatcatgcgg tccttattgg tatagttcgc atttctttca taagccctgt
     1261 atacttcatt acctacagaa gcgtaagtga aagcggtgaa atcaaaattc ttaaactcca
     1321 ggttcacatt ccatccgagg gtgaattcag ggaacggatc accgatcttg gttttatccg
     1381 catcattgat cacaccatcc ttgttcatat ccacaaaacg gatatcaccc ggctgggcac
     1441 ctgtctgtgt aggaccggca tttacttcgg tataattctg gaagagaccg ttggttttga
     1501 aaccgtagaa ataaccaggg gtctgtcctt tttcaaatac cgttacattc tgtgactgac
     1561 cgttgaaata gaaaccaccc aggattttcg ctgtgccatc attgttggtg gcagtcactt
     1621 catttttaga ggtagtgaag gtcacactag tgttaaattt gaaggattta ccgaatgttt
     1681 cattaaagga aagcatcagg tcgataccac tgctcttggt agaaccgata ttcgccgtag
     1741 ggaaaggtac ggtacccagg tacagggaac ctgatggagt aaacagtaaa ccttccactt
     1801 ctttctggaa ataatcggca gaaatggtga atcttctttt gtagaaggtc atatcaaaac
     1861 ccacattcgc ctgcaattgt ttttcccagg ccagggcgtc attggcggct gaacctacgg
     1921 tagaaccggg atagaaaata tcaccgaatg tgtatccgtt actgttggcg gtaggaccat
     1981 aactcggtcc gccggttaca atacttacaa actgaggatc tacgttttcg tttccgatgg
     2041 agccgtagct accgcggatc ttcaggtgat cgatgaattc agatttaaag aaattctctt
     2101 tagacaccac ccatcccagt gaaccggaga agaagttggc gtatttgtta ttcttaccaa
     2161 atgcatagga tccgtcgcgg cgggccgtga aggaagccag gtatttttcc tggaagtcat
     2221 agttgatccg ggagaagtaa gaaaggttac ggcggaaata ctggtaataa taaccggtca
     2281 ccgcattggt attggtggcg gtattgttac cggtagctgc ggtgaagtcc gcaaattccc
     2341 atgagttaaa cggtacatcc tggcggctgg caccagcggc attgccggat gttttggcca
     2401 tagagaaacc gagtacggtt tcaaagttgt ggtcttcttt tactttgaaa ttatagttcg
     2461 caaaagattc ccaggtccag ttgaagttgc tggtcttttc gtgagataca ctgttgaacc
     2521 ggcctgtaac ggtcgatccg tccgcattca tggaattctc tacgttcagc ggaccataga
     2581 ataccagcgg gctgaaggat ttggcattcc catcatattt tgtataacca aaacggctgg
     2641 tgagtttcag gttcttcagt acatcatatt gtaattcgcc tttaccataa agtttgttcc
     2701 ctacattctt attaaaggta ttatccagtt tggtcagcgg gttgaagatc tcggagagca
     2761 gcaggttgct gtatccgtaa gtacccactg tattgggaac ggtgttgtat ttaggtacgg
     2821 tgggatcgaa gttgatcgcg gaacccagta cactgttaaa ggagttttcc tggatgcctc
     2881 tgccatccag taataccgct gtggtattga ggatgaattt caggcggggg gccaggtcaa
     2941 aattcaggtt ggcggtcagg ttaccgcggt taaacctgga tttatcatta ccacccacga
     3001 taccgccctg gctgagccag ccaccggaaa ggaaataaga cattttttcg ctgccgccgc
     3061 gggcagatac gttatgggac tgcatactgg ccattttgaa tacttcattc tgccagttgg
     3121 tacccacacc taccgtactc aggttgggga aaatcacctg gccaccggcc gtggtgcttc
     3181 cttcattgat gatggcggca tattcagaag cgttcagcac acccagggtg ttcaccactt
     3241 cctgtactcc ataattactg ttgaacgtga tatcggtttt ctggttcttg cggccattct
     3301 tcgtggtcac cacaattacc ccgttacccc ctttcacccc gtagatggcg gtagtagccg
     3361 catctttcag caggttgatg gattcaatgt cagcggcatt aatggagttg aaatcagtca
     3421 gtgactgggg aacgccatcg atgatcacca gcggatcggt accggagaag gagggaatac
     3481 ctcttaccag tacggtgggt ttggcacccg gtgtaccact gttaaccacc accacgccgg
     3541 aagcacggcc ctggagggct tcttctaccc ggacgggttt cagtttttca atgtcagcac
     3601 ttttaatggt agagatggca ccggacactt tcgtgacctt ttgggtacca taacccacga
     3661 ccaccacatc cgtaagggtg ctggttgtgc tttcttctaa ttggatgttg accgccgatt
     3721 tgttgtcgga gaccgtaaac tcaacctgtt tcataccggt gtagctgaat accaggacgg
     3781 acccggcttt cgggatcgca atggtaaact ggcccttcgc atctgtcatg gtattggtac
     3841 gggtaccttt taccgagatg gtggcgccaa ccaaaggctc gccggttgat ttgttggaaa
     3901 cggtgccgga aactggcagg ttttgtcctg aggccatctg aagcaggcct gtcaatagga
     3961 cacagagact cagcaatgca gtttttaatt tcatatagca attattttag cgaacaaaaa
     4021 tgctttttaa agcccggcca agttacgcct gtaatccact tttttgaaat tattacctac
     4081 aacaactata catcaaaaaa cgtaactaat tgatattgca ataattaaac tacatcattc
     4141 ctacatcaaa tgcttttccc tactttcaca ttacgaatca ttgccccatc atgagaaaat
     4201 ttatcctgct cgtttttttg cttccggtta cggtcttttg ccaaaataca attggcttac
     4261 cagacattat aaattactca aaacaggcat atgcggccgg tctacaaaac tgggatatcc
     4321 gccaggaccg gcaagggatc ctctatctgg ctaataatga aggggtactg agctttgacg
     4381 ggaaatattg gaacctttac ccgcttccga ataaaaccat tgtccggtcc gtcgaaaccg
     4441 gtgccggaga ccgggtctat gccggcggac aagacgaact gggctatttc tcccctggcc
     4501 ggaacggaag cctgcgcttt acctccctcg ttgaactgtt gcctgaaaaa gagcgttctt
     4561 tcggggatgt atgggatatc tgtgtatata agaaagatgt ctacttccgg acaccgggaa
     4621 aaatattccg gtacaccgga aatgccatgg ccgtgtttac cgccccctcc gagtggtcat
     4681 acctcggtgt ctgcggcgac cgtctttttg cccatgatta tgtgaatggg atcatgacat
     4741 tcaataataa tatctggagt cctgtgcaga ccagcaatca gctgccgggc tccgacccca
     4801 ttacggcgat tttgccaatc gggcgtgaca gcagcctggt gaccaccctc aaaaacggga
     4861 tctatatatt aagtaaggag ggagctgtaa aactgaattc gccggccctg gccctgatcg
     4921 aaaaggaccg gatctatgca gcaacggcaa ttaatgcaga atgggtggcc ctcgccacga
     4981 ataataatgg cgtgcatatc gtagacacca ggggcaacct gatccagcag ttttcccggt
     5041 ctgaaggact tcagaataat aatgtactca gtattttcct ggaccggcaa cagaatctct
     5101 ggctgggact cgataatggc atagacttta ttgcctataa cagtgctatc aaacgaatca
     5161 gtccttattc gcaggacgga tccggttact cggcaattat ccataaggat gtattatata
     5221 caggcacttc tgccgggctt tattcggtac cgcttcagcc tttgcaggat tatagtttca
     5281 gcaaaggaca gtttacacag gtaaccaata ccaccggcca ggtatgggga ctggccacta
     5341 tcaataacac cttattactc gggcatcatg aaggtgcatt tattgttgac ggaaatacgg
     5401 ccaccccttt ctttagttcg gaaaaagggg tatggaactt catcccgctt tcaaccgtgt
     5461 ttcccacggc ccggatgatc accggcactt accgggggct tagtttattt gattacagca
     5521 ataatcggtt tattccggcc gaaaatgtaa aagggctgga tgaatcctgc cgctttgtcg
     5581 ccatcgataa agacaatgcg gtttgggtat ctcaccccta ccacggcgtg taccggttga
     5641 atccggatgg caacggccag tatcagtcta aactttacac cgccgccaat ggacttccgt
     5701 ccaccctcaa caaccacgtt tataaaataa ataatgaagt ggtggtggcc accgaaaaag
     5761 gcgtgtatgg ttttgatccg gccaaagacc ggttcatccc ctctccctat taccaggaat
     5821 tactcgggca acaaagcatc cggtacctga aagaagatcc cgagggaaat atctggttta
     5881 ttcatgataa acaactgggg gtgctggacc gacgtgataa aaaaccgcaa ctgattcacc
     5941 tgccggaact gagcagtaaa atgctgagtg gttttgaatt tatctacccg gtgaacaata
     6001 acaatgtatt tatcggggcg gaaaagggat tcttcctgat caactatgaa aagtacaaaa
     6061 agaatatccc ggtcctggaa gtgatggtca ggaatgtgcg catcttcaac cgccgtgaca
     6121 gcctgctgta tggaggatat gcggccgatt ccggcaaaag tgctcagcca tccatcgcac
     6181 atcagtgggg aacgctgcat ttcgaatatt catccccgtt atttggccag caaaccagcc
     6241 tggaatacag ttgtcgcctg agaggctttg atgacaactg gagtgagtgg tcggccaaga
     6301 cggaaaaaga gtacacccac ctgccggcag gcagttatca ttttgaagta aaagtgcgaa
     6361 acaacctcgg caatgaatcc gccccgcata tttactcctt ccggatcctg cctccctggt
     6421 accagacact ctgggcctac ctggtttatt tcctgatcgg
//
LOCUS       DAOPMW010000170         6452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13778_length_6452_cov_6.531968, whole genome shotgun sequence.
ACCESSION   DAOPMW010000170 DAOPMW010000000
VERSION     DAOPMW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13778_length_6452_cov_6.531968"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLU11_08990"
     CDS             complement(<1..719)
                     /locus_tag="PLU11_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPN59296.1"
                     /translation="MSKDDQDMNIENFLMRSEDDMDFLPIIPLNEGDQEDPNGVEIPA
                     EIGLLPLRNTVLFPGVVLPITVGRDKSIKAVNDAYRGDKLIGVIAQKDSNVEDPEVKD
                     LEKIGTVARIVKMIKMPDGGTTIIIQGKSRFLVEAITGEDPYFKAKIQKLEEEESPKD
                     EDFNAYVANIKDLAADIVQLSPNIPTEASIILRNIENPSFLIHFVSSNLNTDIKDKQR
                     LLELNHIRERADLLMQLLQKE"
     gene            complement(897..1481)
                     /locus_tag="PLU11_08995"
     CDS             complement(897..1481)
                     /locus_tag="PLU11_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPN59297.1"
                     /translation="MKTKLLSLFALVLMSQAAMAQFHLGVKAGTNITKVDGKSFKDEF
                     RYGYHLGGFAEIRMGNKLVLQPEVLWNQYATRVDSNFSHVYQDVFDGNSNVKLNYLSI
                     PILVNYKLIGNFISLQAGPQFGVLIDQNKTLLQNGGNAFKQGDFSMLAGVLVRMGPIR
                     VNGRYAIGLNNISDIPDDNKWKSQGFQLSVGLAL"
     gene            complement(1662..2693)
                     /locus_tag="PLU11_09000"
     CDS             complement(1662..2693)
                     /locus_tag="PLU11_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59298.1"
                     /translation="MIKKWSICLAFLFNYVTGTAQVLPADTVLIKADSSLFAIDTSLD
                     YDFLFRDFDAFMDSLLTPRSYSLVSLSVGKGYYNYSSKTDYSISTSKQLNYSPTLGFY
                     HRGGFGITGTGYIVHDGENMNFYQAGITPSFDYLKNRSIATGISYTRYLTRDSLPFYT
                     TPLQNELYGYFMYRKSWFRPTVAASYGWGSRSDYMQREELIQDLRLRRRGFTYINTEE
                     TVSDFSIMASIRHDFYWLEVFSARDHIRLTPQLAFTSGTQKFGFNQSSSTYATTIRTG
                     SNVLFNTDNVYLDDQLDFQPLSLTMYLRGEYSIGKFFFQPQLVLDYYFPADTQNFNAL
                     FTVNAGFFF"
     gene            complement(2843..4057)
                     /locus_tag="PLU11_09005"
     CDS             complement(2843..4057)
                     /locus_tag="PLU11_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN59299.1"
                     /translation="MFLIDLYFFQALKVLTQSASPRTRLIIHSGYWTLSALAIVMLIL
                     LPYLQMDKAAKLAKSTIFSMIAGLFFAKLIASLFFLVDDLRRGVQWAGEKILTRPKEV
                     GAAALTGEGITRSAFMSWLGVLAGSGLFGTLVYGFGNKHRYQVSKVKLNYPNLPAAFR
                     GTKIVHISDIHSGSFTDKEAVMKGVYKILAEKPDMILFTGDLVNNLATEMEDYKEVFN
                     KLEAPMGVYSTLGNHDYGEYVQWDSPDQKAANLESLKKVHADMGWRLLMNEHVVLEKE
                     GQQIALLGIENWSAKARFPKYGDLSKAHAGAAAYPFKILMSHDPSHWRAEVMEKYPDI
                     DLTLSGHTHGMQFGVEIPGLKWSPVQYMYNEWAGLYEKEKQKLYVNRGFGFIGYPGRV
                     GILPEITLIELA"
     gene            4261..5064
                     /locus_tag="PLU11_09010"
     CDS             4261..5064
                     /locus_tag="PLU11_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN59300.1"
                     /translation="MARIIGVANQKGGVGKTTTAINLAASFAVLEYKTLLVDGDPQAN
                     STTGVGFDLHNVTQSLYDCMVNQARAKDVILKTEIPNLDLIPSHIDLVGAEIEMINYP
                     SREMVLKNLLDGIRNDYDFIIIDCSPSLGLITVNALTAADSVVIPVQAEFFALEGLGK
                     LLNTIKIVQSRLNTALVIEGILMTMYDGRLRLCNQVVSEVRRHFDEMVFSTIVHRNAR
                     LAEAPSAGKPVILYDAASKGSMNYLNLAKEILQKNDMTKMTQEERILEI"
     gene            5135..6055
                     /locus_tag="PLU11_09015"
     CDS             5135..6055
                     /locus_tag="PLU11_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPN59301.1"
                     /translation="MTTPKQNNKDLLGKGIRSLLQSIDSDLKTGSGQLKQNVVENATG
                     ISRLSVDEIETNPKQPRRDFDEKALQELAESIRLHDIIQPVTVSRMANGKYRLISGER
                     RLRASKLAGLKDIPAYVRQANDQQLLELALLENLQREDLNAMEIALSYKRMMEELDYT
                     QEQVAERMGKDRSTVANFIRLLKLPPDIQLAVRSNELSMGHARALINVDTVDQQLYIY
                     KEIKNKGLSVRQTEALVRNLYKQGDAVKKTAKSQLPSAFQRIEDKLAGYFSTRVKLKN
                     SRNGSGQIIIEYYSQEELTKILRQMDAPLD"
     gene            6062..>6452
                     /locus_tag="PLU11_09020"
     CDS             6062..>6452
                     /locus_tag="PLU11_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPN59302.1"
                     /translation="MRILFSLLCFLLLSSANLCAQEKELPVTEKKDSLPVIKKAATGS
                     SRYDSALKAHSPRKAAIRSAILPGWGQIYNKKYWKLPIVYGALGTSGGIFFYNLKNYR
                     DTRFAYRVKYNMQYNQTDSALYSLIRAN"
BASE COUNT         1842 a         1475 c         1485 g         1650 t
ORIGIN      
        1 aattccttct gcaataactg catcaggaga tctgcccgtt cgcggatatg gttcagttcg
       61 agcaaccgtt gcttatcctt gatatcggta ttcaggttac tggatacaaa atggatcagg
      121 aaagaaggat tctcaatatt ccgtagaata atagaagctt ctgttgggat attgggtgaa
      181 agctgcacaa tatccgccgc cagatctttg atatttgcca cataggcatt gaagtcctcg
      241 tctttcgggg attcttcttc ttcaagtttc tgaatttttg ctttgaagta aggatcttcg
      301 ccggtgatgg cttctaccag gaaacggctc ttcccctgaa tgataatggt ggtgcctcca
      361 tcgggcattt tgatcatttt cacgatccgg gccactgtac cgattttctc caggtctttc
      421 acttccggat cttccacatt actgtctttc tgagcaatca ccccgatcag tttatcgccc
      481 cggtaagcat catttacagc ttttatgctt ttatcacggc caacggtaat gggcaaaacc
      541 accccgggga ataaaacggt attgcggagc ggcagtaaac ctatttcggc aggaatttcc
      601 accccgttcg ggtcttcctg gtcaccttca ttcaatggaa taatgggcaa aaaatccatg
      661 tcatcttctg aacgcatcaa aaagttctca atattcatat cctgatcatc tttagacatt
      721 ctgtcatttt atccggacca acaaggtccg gggggtaaaa gtatagcgtg aattcagtat
      781 tgccaagtat cgtgcctgtc atttcgggac tgacaaacta tgtatttgca ccggaacctc
      841 ttatgcataa aaaaaacctc cccgggggga ggcttttcat agctatacga acggtattaa
      901 agggctaatc ccacggataa ctggaagccc tggcttttcc acttgttgtc atcgggtata
      961 tcgctgatat tattcagtcc gatcgcgtag cgtccgttga ccctgatcgg acccatccgg
     1021 accagcacac cggccagcat ggagaaatcg ccttgtttaa aagcatttcc accattctgg
     1081 agcaaggttt tgttctgatc gatcaatacc ccaaactgag gaccggcttg cagagaaata
     1141 aaattgccga tcagtttgta attgaccagg atcgggatgg acaggtaatt cagtttcaca
     1201 ttactgttgc catcaaatac atcctggtaa acatgactga aattactgtc gacccgggtt
     1261 gcgtactggt tccagaggac ttccggttgc aacaccagtt tgttacccat acgaatttcg
     1321 gcaaaaccac ccaggtggta accgtaacgg aactcgtctt taaaggattt gccatcaacc
     1381 ttggtaatgt tggtaccggc tttcacacct aaatgaaact gggccatggc tgcctgtgac
     1441 atcagtacca gggcgaaaag cgaaaggagt tttgttttca tgcgcatttt tttaattgtt
     1501 ttttggaatg gcttgagacc ggttatattt tttcaagtat agtgccagtc acccttgccc
     1561 gctgttaaaa ttgatttaac gtaaataagt acccgctaaa acataaattg taacagggcc
     1621 attgcgattg aaatgtgaat gttttctaaa agaaaaatga ctcagaagaa aaaaccggca
     1681 ttcaccgtga agagggcgtt gaagttttgg gtatcggccg ggaagtaata atcgagaact
     1741 aactgcggct ggaagaaaaa tttaccaatg gaatattccc cccgcaggta catggtcagc
     1801 gataaaggtt gaaaatcgag ctgatcatcg agataaacat tatcggtatt gaagagtaca
     1861 ttactgccag tgcggatagt ggtggcatac gtactggaag actggttaaa cccgaatttc
     1921 tgtgtgccgc tggtaaaggc cagttgcggg gtaaggcgga tatggtcacg ggcagaaaac
     1981 acttcaagcc agtaaaagtc atggcggata gaagccataa tggaaaagtc actgactgtt
     2041 tcttcagtat taatataagt aaaaccccgc cggcgcagcc gcaggtcctg gatcagttct
     2101 tcacgttgca tataatcgct gcggctgccc caaccataac tcgcggcaac agttggtctg
     2161 aaccaggatt tacggtacat aaaatagcca tagagttcat tctgcaatgg ggtggtataa
     2221 aagggtaatg aatcgcgggt gagataacgg gtataggaaa taccagtggc aatactccgg
     2281 tttttcaaat agtcaaatga aggcgtgatc cctgcctggt aaaaattcat attctcccca
     2341 tcatgaacaa tataacctgt accggttatt ccaaaacctc ctctatgata aaatccaagc
     2401 gtaggcgaat agtttagttg ctttgaagtg ctgatggaat agtcggtctt actggaataa
     2461 ttgtaatacc ctttcccaac ggataaactg accagtgaat aactccgggg ggtgagcaac
     2521 gaatccataa aagcatcaaa atcccggaat aaaaaatcat aatccaggct ggtatctatt
     2581 gcaaaaaggg aactgtctgc tttaatcaat acagtatctg ccggtaatac ctgggcggtt
     2641 ccggtgacat aattaaagag aaaggccagg catattgacc attttttaat catcaggcag
     2701 tagggtggcc tatttgttta cagaaaataa agcccttatt cttctgtcag accggaagca
     2761 ggccagaaag tttaaagccg gtacccgacg aagataaaaa aggatttctg ccagtacact
     2821 aaaaattaag ggattatcat ttctaagcca gttcgatcag ggtaatttcg ggtaaaatcc
     2881 ccacccggcc cggatagccg ataaacccga atccccggtt gacatagagt ttttgctttt
     2941 ctttttcgta cagtccagcc cattcattgt acatatattg caccgggctc catttcaggc
     3001 cgggaatttc cacaccaaac tgcatcccat gggtatgtcc tgataaagtg aggtcaatat
     3061 ccgggtattt ctccatcact tcagcccgcc aatgcgatgg gtcatggctc atcaggattt
     3121 tgaagggata ggcggcagct ccggcatgcg ctttactaag gtctccgtat ttgggaaaac
     3181 gggctttggc actccagttt tcaattccaa gtaaggcgat ttgctgacct tctttttcca
     3241 ggaccacatg ctcattcatc agtagccgcc agcccatatc cgcgtgtact ttcttcagac
     3301 tttccaggtt ggctgctttc tgatcagggc tatcccactg cacatattct ccataatcat
     3361 gattacccag ggtggaatac acacccattg gagcttccag tttattaaac acctctttat
     3421 aatcctccat ttcggtggcc aggttattca ccaggtcacc ggtaaacagg atcatatccg
     3481 gtttttcagc cagaatttta tatacaccct tcatcacggc ttctttatcg gtaaaactgc
     3541 ccgagtggat atcggaaata tggacaattt tagtgccccg gaaggctgcc ggcagattcg
     3601 gataattgag ttttacttta cttacctggt aacggtgttt gtttccaaaa ccatagacca
     3661 gggtaccaaa caggccgctt ccggccagga cgcccaacca gctcataaag gccgaccggg
     3721 taatgccttc cccggtcagg gcagccgccc ccacttcttt tggtctggta agtatcttct
     3781 ccccggccca ttgaacgcct ctacgcaaat cgtccaccag gaaaaacagg gaggcaatca
     3841 gtttggcgaa aaacaggcca gcaatcatcg aaaaaatagt gcttttggcc agtttggccg
     3901 ctttatccat ctgcaggtag ggtaaaagga tcaacataac aatggccagc gcagaaagcg
     3961 tccaataccc ggaatggata atcaaccggg tacgggggct ggcggactgg gtcaatacct
     4021 tcagggcctg aaagaaatag aggtcaatga ggaacataaa gcccagaaaa acccaccaaa
     4081 acggagaact acgcatataa gtgtcaaaat taagcgtcca tgaataatag gcagtctttt
     4141 gtcgaacgct tatttttgcc agcatgcctt tttaccggcg taaaacttaa atttgcgttc
     4201 ttgtgatcag ggggcaattt taccatattc aatcacttac ttcaaccagt tttcggcaaa
     4261 atggcaagaa ttatcggtgt agctaatcaa aagggaggtg taggcaaaac caccaccgcg
     4321 atcaacctgg cggcaagttt tgctgtgctc gaatacaaaa ccctgctggt ggatggtgat
     4381 ccgcaggcca acagcaccac aggtgttggc tttgacctgc acaatgtcac ccagagtctg
     4441 tatgactgta tggtcaacca ggcccgtgca aaagatgtga tcctgaagac agaaatccct
     4501 aacctggatt taattccttc acatattgac ctggtgggtg ctgaaattga aatgatcaat
     4561 taccccagcc gtgaaatggt gctgaagaat ctgctggatg gaatacggaa tgactatgac
     4621 tttattatca tcgactgctc tccttctctt ggattgatta ccgtaaacgc attaaccgct
     4681 gcggattccg tggtaattcc ggtacaagct gaatttttcg ccctggaagg attaggcaaa
     4741 ctgctcaata cgatcaagat tgtacaaagc cggttgaata ctgcattggt aattgaaggt
     4801 atcctgatga ccatgtatga cggacgcctt cgtctctgca accaggtggt cagtgaagta
     4861 cgccggcatt ttgatgaaat ggtattcagt actattgtac accggaatgc aagattagcc
     4921 gaagccccga gtgccggcaa acccgtgatt ttatatgatg cagccagcaa aggctcgatg
     4981 aactatctga acctggccaa agagatattg cagaagaatg atatgacgaa gatgacgcag
     5041 gaagagagaa tacttgaaat ataagtacga aatacgaagt acgaagtacg ggctgtttcc
     5101 tgacaactga caaccgacaa ccgacaaccg aacaatgacg actccaaaac aaaataacaa
     5161 agacctcctc ggcaaaggaa tccgatcctt attacagagt attgattctg atctgaagac
     5221 cggcagcgga caattgaaac agaatgtggt ggagaatgcc accggcatct cccgtttatc
     5281 ggtggatgaa attgagacca atccgaagca gccccgccgt gactttgatg agaaagcatt
     5341 gcaggaactg gcggaatcga tcaggctgca tgatattatc cagcctgtga ccgtatcgcg
     5401 tatggcgaat ggtaagtatc gcctgatttc gggggaacgc agattaaggg catccaaact
     5461 ggccggactg aaagatatac cggcttatgt acgacaggcc aacgatcagc agttacttga
     5521 attggccttg ctggaaaact tacaacggga agacctcaat gccatggaaa tagcactgag
     5581 ctataaacgg atgatggagg aactggatta tacccaggaa caagtggctg aacgaatggg
     5641 caaggaccgg agtacggtgg ccaattttat ccgtttgctc aaacttccac ctgatatcca
     5701 gctcgcggtc cgcagcaatg aactgagcat gggtcatgcc cgggccctga tcaatgtgga
     5761 tacggtggat cagcaacttt atatttataa ggaaataaaa aataaagggc tttcggttcg
     5821 ccagacagaa gctctggtcc gcaacctgta taaacaaggt gatgctgtta aaaaaacggc
     5881 aaaaagccag ttaccctccg catttcagcg aatagaagat aaattagcgg gttatttcag
     5941 tacaagggtt aaactgaaaa acagccggaa tggaagtggt cagattatta ttgaatatta
     6001 ttcccaggaa gaactgacca agatcctgcg gcaaatggat gccccgcttg attaatttat
     6061 catgagaata cttttctctt tactgtgttt tctgttgctc agctcagcca acctctgtgc
     6121 acaggaaaaa gaattgccgg taacagaaaa gaaggattcg ctgccggtaa ttaaaaaagc
     6181 tgccactggc agcagtcgct atgattctgc cctgaaagcg catagtcccc ggaaagccgc
     6241 gatccgctca gccattctgc ccggctgggg acagatatac aataaaaaat actggaaact
     6301 gccgattgtt tacggcgccc tgggtaccag cggcggcatc tttttctata acctaaaaaa
     6361 ttacagggat acccgttttg cgtatcgggt aaaatacaat atgcagtata accagacgga
     6421 cagcgccctc tattccctga tccgggccaa cc
//
LOCUS       DAOPMW010000171         6406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13905_length_6406_cov_10.566210, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000171 DAOPMW010000000
VERSION     DAOPMW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13905_length_6406_cov_10.566210"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..693)
                     /locus_tag="PLU11_09025"
     CDS             complement(214..693)
                     /locus_tag="PLU11_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPN59303.1"
                     /translation="MKFITTIALALFSLQTMAQDIPKAVLKDTTGKAADTNYVIIHKD
                     PRVDLLIKKQAQINEETSRAGRRTDKGFRLLLISTSSRDEALSAKTKVYTYFPELKAY
                     LWHQSPYYKVKAGNFKDRKDAETYQKKLNIYFPKGVFIMNDIIEVKPPKPGEEEISL"
     gene            complement(800..1681)
                     /locus_tag="PLU11_09030"
     CDS             complement(800..1681)
                     /locus_tag="PLU11_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN59304.1"
                     /translation="MRLEVLKTYKIYIGGQFPRTESGRYYSPVNKTGKKLANVCLCSR
                     KDFRDAVVAARGAFSGWSNRAAFNRSQILYRMAEMLEGRKAQFIEELILQGASKVAAE
                     KEVNLATDRLIYYAGWCDKFQQLFSAVNPVATPHFNFSVPEPTGVVSIIAPQGDSLLG
                     LVSLIAPVIAGGNTCVVLASETKPLCAVTFAEVLNSSDLPGGVVNILTGKPAELVSWF
                     TDHMDVNATLYCESDSIVQKMIREKSAVNLKRVHFYDKINWYTDEGQSPYFIMDTQEI
                     KTTWHPVENIAGAGGGY"
     assembly_gap    1788..1887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1900..2319)
                     /locus_tag="PLU11_09035"
     CDS             complement(1900..2319)
                     /locus_tag="PLU11_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN59305.1"
                     /translation="MATIEKFEDIISWKEARDLNKTIGNLIDTGRFKNSYRLINQIEG
                     SAGSIMDNIAEGFERGGNKEFIQFLYIAKASCGELRSQLYRALDRNYIAQNEFEQIST
                     HAKKISALIQKFITYLENSDTKGIKYKKRTGEENKST"
     gene            complement(2375..>3181)
                     /locus_tag="PLU11_09040"
     CDS             complement(2375..>3181)
                     /locus_tag="PLU11_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN59306.1"
                     /translation="TGAGATGAALVNHPDINKIAFTGSTDVGKIIQRAVAGTNKRVTL
                     ELGGKAANIIFEDAPLDQAVEGVINGIFFNQGHVCCAGSRLYVQESVAKEVIRKLKDR
                     MESLIVGDPLDKNTDIGAINSKQQLATIQKYIGIGKKEGAEMYESHCRLPNKGFFCRP
                     TLFTNVAQSNRIAQEEIFGPVLSILTFRTDDEVIEKANNSPYGLSAGVWTDKGSKIFN
                     MTKRMRAGVIWANTFNKFDPTSPFGGYKESGFGREGGLHGLLPYLELTSL"
     assembly_gap    3182..3281
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3282..3748)
                     /locus_tag="PLU11_09045"
     CDS             complement(<3282..3748)
                     /locus_tag="PLU11_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN59307.1"
                     /translation="MAKKSTPAKSNTIRLKFDSSWSYAPAPESKSAASIDKQYDLFIN
                     GEWQKPLSKKYFDTINPATEEKLSEVADANAADVNKAVVAARAAYEKVWKKMPAKERA
                     KYIFRIARMVQEKARELAIIETLDGGKPIRESRDFDVPTAANHFFYYAGWADKL"
     gene            complement(3890..4831)
                     /gene="deoC"
                     /locus_tag="PLU11_09050"
     CDS             complement(3890..4831)
                     /gene="deoC"
                     /locus_tag="PLU11_09050"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPN59308.1"
                     /translation="MKQESTNMAIPKEKLIIPDFTLSPRIDQVGVEERASRFTKRSIK
                     NETKLNGLKLVLNMIDLTTLEGKDTDGKVKQLCYKAMHPHDALPGLPTVAAVCVYPTM
                     VKVAKKALEGSGINVASVATAFPSGQAPRDVKIRDTKYAVANGADEVDMVISRGKFHS
                     GEYNFVFDEIAAIKEACGPARLKVILETGELGTLDKVRRASDIAMHAGADFIKTSTGK
                     IQPAATMPVTLVMLEAIRDFYYKTGKMIGMKPAGGISKSKLALHYLVMVKEVLGDDWL
                     TNEWFRFGASSLANDVLMQLVKMETGLYQAGNYFSAD"
     gene            5005..>6406
                     /locus_tag="PLU11_09055"
     CDS             5005..>6406
                     /locus_tag="PLU11_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN59309.1"
                     /translation="MRKLKRLAGTLYAVLAAMTAFSQPTFPENGIADPRHGHFAFTHA
                     TLVKDAATTLTDATLVIKDGRIVAAGTGLPVPAGAVEVDCKGKFIYPSFIDIYTDYGT
                     TPAQRAAPAGGGGGFFAQQQFDNAVKGPFGWNQAIKSDVDVYKVFGVNDATAKSMREA
                     GFGTVLTHMKDGVARGTGAVVTLANAKENLVIIKEKASAHYSFNKGTSTQSYPNSLMG
                     YISLLRQTFLDASWYKTKPVQEGFNATLQSWNDIQGLPQIFEANDKWNGLRADRIGDE
                     FGVQYIIKGGQNEYQRIKEVKATNATYILPLNFPQGQDVEDPNDARMVSLEDMKHWEM
                     APSNAAAFEKAGVPFCLTTSDLRSTASFWINLRKAMEYGLSETKAMDALTKVPAQALG
                     IYDKVGSLEAGKLANFLVTNGPIFSEKTNILYNYVQGIKYNVKDDTNIAGTYNLTINT
                     PAGEEKYTLDVKSNSSV"
BASE COUNT         1486 a         1406 c         1481 g         1833 t
ORIGIN      
        1 gggattccca gttcggagag ccgttgctgt acgaattttg acgtttcaaa ttcctggtag
       61 ctcaattcag ggtgtgcatg aaggtgatga cggacttcaa taaactcagg cgcgtatttt
      121 ttggcgaggg cttttatttt agcttccatg gggcaaaaat aaacaaacca ttccttaagg
      181 ggaatggttt gttcagttct ttatagagat atctcagagt gagatttcct cttcgccggg
      241 tttcggcggt ttcacttcaa tgatatcgtt catgataaag acccctttcg ggaaatatat
      301 attcagtttt ttctgatagg tttctgcatc tttccggtct ttgaaatttc cggctttaac
      361 cttgtaatat ggggattggt gccagagata ggctttcagt tccgggaagt aggtgtatac
      421 tttggtcttg gcactcaggg cttcatcccg gctgctggtg ctgatgagca acaggcggaa
      481 tcctttgtcc gtacgtctgc cggcccggct ggtttcctca ttgatctggg cctgtttttt
      541 aatcaggaga tccacccgtg gatctttatg aatgatcacg taattggtgt ctgcggcttt
      601 gccggttgtg tctttgagaa cagccttagg tatatcctgg gccatggtct gcagactaaa
      661 taaggcgagt gcaatggttg taatgaattt catcatgatg aggttgcaaa tatcttacca
      721 atccggataa aaaagtacaa gggggctgtt aagtcgtgga taaagttcat aactgcttca
      781 ttctctttaa tccctgcggt tagtaaccac ccccggcccc tgcaatgttt tccaccgggt
      841 gccaggtggt ttttatttcc tgggtatcca tgataaaata gggagactgg ccttcgtctg
      901 tgtaccagtt gattttatca taaaaatgaa ctcttttcag attgaccgct gatttttccc
      961 ggatcatttt ctgcactata gaatctgatt cgcagtaaag ggttgcattt acgtccatat
     1021 gatcagtgaa ccaggagacc agttcagcag gtttgccggt taggatattc accacgccac
     1081 cgggcaggtc ggaactgttg agcacttccg cgaatgtaac agcgcagagt ggttttgttt
     1141 cggaagccag taccacacag gtattacccc cggcaatcac cggggcaatg agggatacca
     1201 gtcccagcag tgaatcaccc tgcggggcaa tgatggatac cacaccggtg ggttcgggta
     1261 ccgagaaatt gaaatgcggg gtggcaacag gattcacggc actgaatagt tgctggaatt
     1321 tatcacacca gccggcataa tagatcaggc ggtcggtggc gaggttcact tccttttccg
     1381 cagctacttt cgatgcaccc tgcagtatca gttcttcgat aaactgcgct tttctgcctt
     1441 cgagcatttc ggccatacgg tagagaatct ggctgcggtt gaaagcggcc cggttgctcc
     1501 atccgctgaa ggccccgcgg gcagccacca cggcatcccg gaaatctttc ctggagcata
     1561 ggcatacatt ggccagtttt tttcccgttt tattcacggg actatagtaa cggccggact
     1621 ctgttctggg aaattgtccg ccgatgtaga ttttgtaggt tttgagtact tcgaggcgca
     1681 tggtggtaga ctttataggt ttgaaaggtt tcagaggttt aaaaggttta aaaggtttga
     1741 aaggttaggt ggatttgttt tcttccccgg ttcttttttt atattttnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnagg tttgaaaggt taggtggatt tgttttcttc
     1921 cccggttctt tttttatatt ttatgccttt tgtatctgag ttttctaggt atgtgatgaa
     1981 tttttgtatc aaggcgctaa ttttttttgc atgggttgag atctgttcaa attcgttttg
     2041 ggcaatatag tttctgtcaa gggccctgta tagttgtgac cttaattctc cacaggaggc
     2101 tttcgcgata tacaagaact gaataaattc tttatttccg cctctttcaa aaccctctgc
     2161 aatattgtcc attattgagc cggcagaacc ttcgatttga ttaattaagc ggtaactgtt
     2221 tttaaaccgc ccggtatcta ttagattccc aatcgttttg tttaaatctc tcgcttcttt
     2281 ccatgagata atatcttcaa acttctcaat tgttgccatt tcccaacctt ttaaaccttt
     2341 taaacttttt aaacctttta aactttttaa acctttacaa acttgtcaac tccagataag
     2401 gcaacaaccc atgtagtcca ccctccctgc caaacccgct ttccttatat ccgccgaacg
     2461 gcgacgtggg atcaaattta ttaaacgtgt tcgcccaaat caccccagcc cgcatacgtt
     2521 tggtcatatt gaaaattttg gatcctttgt cggtccatac tccggcgctc aggccatacg
     2581 gtgagttgtt ggctttttca atcacttcat catcggtgcg gaaagtgaga atggaaagaa
     2641 cgggtccgaa aatttcttcc tgggcgatgc ggttgctctg ggccacattg gtaaagaggg
     2701 tagggcggca gaaaaatcct ttattcggga gacggcaatg gctctcatac atttccgccc
     2761 cttctttttt tccgatgccg atatatttct ggatggtcgc cagttgttgc ttcgaattaa
     2821 tcgcgccgat atcggtattc ttgtccagcg gatcacccac gatcagggat tccatgcggt
     2881 cttttaattt gcggattact tctttggcta cggattcctg gacatataac cgggagcccg
     2941 cacaacatac atgaccctgg ttgaagaaga taccgttgat caccccttcc acggcctggt
     3001 caagcggggc atcttcaaaa atgatattgg cggctttacc accgagttct agggtgactc
     3061 ttttattagt gcctgccacc gcccgttgaa taatttttcc tacatccgtg gaaccggtga
     3121 aagcaatttt attgatatcc ggatgattga ccagggctgc tccggtagca ccggcaccgg
     3181 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtttatcg gcccagcctg
     3301 cataataaaa gaaatgattg gcggctgtgg gcacatcgaa atcacggctt tcacgaatgg
     3361 gtttgcctcc atcgagcgtt tcaatgatag ccagttctct tgctttttcc tgtaccatcc
     3421 tggcgatccg gaaaatgtat ttggctcttt ctttggccgg catttttttc cagacctttt
     3481 cataagctgc acgggcagcc accacggctt tgttgacatc cgccgcattg gcatcggcca
     3541 cttcactcag tttttcttca gtggccggat tgatcgtatc aaaatatttt ttactcagcg
     3601 gcttttgcca ttccccattg ataaacaaat catattgttt atcaatgctg gcggcgcttt
     3661 tgctttccgg ggcaggggcg taggaccaac tggagtcgaa tttcaatctt atagtattgc
     3721 ttttagctgg agttgatttt tttgccatgt tttagttcag tttctaaggt ttcaaaggtt
     3781 taagaggttt gaaaggtttc aaaggtttaa gaagtttcag aggtttgagc attgccgtca
     3841 gaaggttaac cttttaaact tcttgaactt ttttaacctc ttaaaccttt tagtcagcac
     3901 taaagtagtt tcccgcctgg tacagccccg tttccatttt cacgagttgc atcagcacat
     3961 cattggccag actgctggcg ccaaagcgga accattcatt ggtcagccag tcatcgccca
     4021 ggacttcttt caccatcacc aggtaatgaa gcgccagttt ggatttggaa ataccgccgg
     4081 cgggtttcat tccgatcatt ttcccggtct tataataaaa atcacggatc gcttcaagca
     4141 ttacgagggt cacgggcata gtggcggccg gttgaatttt gccggtggag gttttaataa
     4201 aatcagctcc cgcatgcatt gcaatatcac tggctcttct tactttatcg agcgtaccca
     4261 gttcgccggt ctccaggatc acttttaacc gggccgggcc acaggcttct ttgatagcgg
     4321 cgatttcatc aaatacaaaa ttgtattcac cggaatgaaa ttttccacga ctgatcacca
     4381 tatccacttc atcggctccg ttggccactg catattttgt atcccggatc tttacatcgc
     4441 ggggggcctg tccgcttggg aaagcggtag ccacagaagc cacattgata ccggaacctt
     4501 ccagggcttt ttttgccact ttgaccatcg tgggatatac acagacggcc gctaccgtgg
     4561 gcagaccggg gagtgcatca tgcgggtgca tggctttgta acaaagttgt ttcactttgc
     4621 catccgtatc ttttccctcc agtgtggtga ggtcgatcat gttcagtacc agcttcagtc
     4681 cattgagctt ggtttcattt ttgatacttc gtttggtaaa gcgggaagcc ctttcttcca
     4741 cccctacctg gtcgatacgc ggggagaggg tgaagtcggg aatgatgagt ttttccttcg
     4801 ggatcgccat attggttgat tcttgtttca aaagtaaaac tatttatggg gattactggt
     4861 gagtaaatat tgaggataac ggactgaagg ttaacggtgt ttttcgcgtc aaaggcccgc
     4921 tgccgttcat ccatttggcg acggcagaac catattttta tgtaaaatcg tttttcgata
     4981 actttcaaca aaatcaacaa ctgtatgaga aaactgaaaa gactggccgg gaccttgtat
     5041 gcggttctgg cggccatgac cgcattttcc cagccgactt tcccggaaaa tggaattgcc
     5101 gatccccgtc atggtcattt tgcattcact catgccacat tagtcaagga tgcggcgacc
     5161 acattaacgg atgccaccct ggtcattaaa gacggaagga ttgtggcggc cggcaccgga
     5221 ttacctgttc cggcaggagc agtggaagtg gattgtaaag gcaaattcat ttatccatcc
     5281 tttattgata tttatacgga ttacggcaca acacctgcac aacgtgctgc tccggcgggt
     5341 ggtggcggtg ggttctttgc ccagcaacag ttcgataatg cggtgaaagg tccttttggc
     5401 tggaaccagg cgatcaaatc cgatgtggat gtatacaagg tcttcggtgt aaatgatgcc
     5461 actgcgaaat ctatgcggga agctggtttt ggaaccgtac tcacccacat gaaagatggc
     5521 gtggccaggg gtaccggggc cgttgttaca ttggccaatg caaaagagaa tttagtaatt
     5581 attaaagaaa aggcttcggc acattattcg tttaataaag gcacttccac ccaatcttat
     5641 cccaattcac tgatgggtta tatttccctg ctgcgccaaa cctttctgga tgccagctgg
     5701 tataaaacca aaccggtgca agaaggcttt aatgccaccc tgcaatcctg gaatgatata
     5761 cagggactgc cccagatttt tgaagccaat gataaatgga atggacttcg tgccgaccgt
     5821 atcggtgatg aatttggtgt gcagtatatc atcaaaggcg gacaaaacga ataccagcgg
     5881 atcaaagagg tgaaagcgac caatgcaacg tatattctgc cgcttaactt tccacagggt
     5941 caggatgtgg aagatccgaa tgatgcaagg atggtttcac tggaggatat gaaacattgg
     6001 gaaatggcgc cctccaatgc cgctgcattt gagaaagcag gcgttccttt ctgtctcacg
     6061 acctccgacc tgcgtagtac cgccagtttc tggatcaacc tccgcaaggc catggaatat
     6121 ggactgagtg aaacaaaagc gatggatgcg ttgactaaag tacccgctca ggcattggga
     6181 atttatgaca aagtgggtag cctggaggcc ggtaaactgg ccaatttcct ggtgaccaat
     6241 ggtcccattt tcagtgaaaa gacgaatatc ctgtacaact atgtgcaagg gatcaaatac
     6301 aatgttaagg atgataccaa tatcgccggt acctataatc tgaccatcaa cacacctgca
     6361 ggtgaagaga aatataccct ggatgtgaag agtaacagca gtgtga
//
LOCUS       DAOPMW010000172         6377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_13993_length_6377_cov_8.539228, whole genome shotgun sequence.
ACCESSION   DAOPMW010000172 DAOPMW010000000
VERSION     DAOPMW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13993_length_6377_cov_8.539228"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PLU11_09060"
     CDS             complement(<1..2270)
                     /locus_tag="PLU11_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HPN59310.1"
                     /translation="MHATPGYKLIYKWLEAKSFRPFLFQEETWTHISQRKSGLVNAPT
                     GCGKTYSVFLGTLIRYINLHPDDYGTRKNAGLQLLWVTPLRALAKDIGRAMEEVLTEL
                     QLPWKVGIRNGDTSIAERARQKKQMPEILIITPESLHLLLAQKGYPALFQSLAVIAVD
                     EWHELLGSKRGVQVELAISRLVHLQENPTQRPMVWGISATIGNLEEAGEVLLSPLQQP
                     CITVTAALQKKIQVESIYPDEIEKYPWAGHLGIKLAHKLIPIIQASGTTLIFINTRGM
                     SETWYQTLLDICPDLAGVIALHHGSIDPELRNWIEDNLHTGKLKAVVCTASLDLGVDF
                     RPVETVIQVGSPKGVARFLQRAGRSGHQPDAVSRIYFLPTHSLELTEAAALKSAIDEN
                     LIESRIPFTGCYDVLLQYLNTLAISEGFTHPSLYHEIRTTYCYRDLTEEEWGQLLHFA
                     TQGGKAMQQYDDFKKVELEDGVYRIKSRKLAMRHRMHIGTIVSDAMLKVKFISGGYIG
                     VIEEYFISRLSPGDVFTLAGRNLEFVMIKDMTVLVRKSSSKKSIIPSWNGGRMPLSAN
                     LGKKLRAALNRYRQGTSTETEELKALHPLFELQAALSHVPAENELLIEQIETKDGFHL
                     FVYPFEGRLVHEAMAAILAWRISRIQPITFSFAMNDYGFELLSDQPIPVDDTNVYDLF
                     TTDGLLLDIQRSVNNTEMARRKFRDVAVIGGLIFQGMPGEKKKARHLQSSASLLFNVF
                     SEYDSDNLLLRQAYQEV"
     gene            complement(2299..3474)
                     /locus_tag="PLU11_09065"
     CDS             complement(2299..3474)
                     /locus_tag="PLU11_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HPN59311.1"
                     /translation="MKIQTWVLFLSFFLVQTAGAQEPAPKFKIAIFAPLYLDSAFDDQ
                     SEYRYAKNTFPKFINPGLEFYEGAQLALDSLNREKINLDVQVFDTRSVHQTVQQQLAN
                     ASVKDINLLIAYCSANEVKSFADAGLQMNIPVINVNLPNDGGVTGNPYYVMLNSTLKT
                     QCEGIYRHIQKFYALDRLIVFRKKGVLEDRIKSYFDEFAKNTMGVPLKLKYIDLTDSF
                     QVNQLSPYLDTLQNTLCIAGSLDENFGKRLALQLAVLKKQNLKATVMGMPTWDAIKDF
                     TRPEYRGIEIVYSTPFYNPRTDKVSQSITNTFNTRMFARPSDMVMRGYETVWRFVKLM
                     LLYKADFSSNITRKEFNVFREMDIQPVLNKQTMTLDYFENKKLFFLKWQDGIIKSVN"
     gene            complement(3486..5021)
                     /gene="guaA"
                     /locus_tag="PLU11_09070"
     CDS             complement(3486..5021)
                     /gene="guaA"
                     /locus_tag="PLU11_09070"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPN59312.1"
                     /translation="MTEKILILDYGSQYTQLIARAVREANVYCEIIPFYKPFTFEPGL
                     KGIILSGSPFSVNEPNAPDTDVANLVSQVPVLGVCYGAQLTAKRYGGVVAKSDKREYG
                     RAILQRKKEDTLFSEVSDQSQVWMSHSDTIKQLPEGFELLADTDSIPVAAFRKAGNDG
                     NPLYGVQFHPEVYHSKEGKKIIHNFLVKICGCSQDWTPAHFITDTVDQLKKQIGDRKV
                     IMALSGGVDSTVAATLIHRAIGDRLFGIFVDNGVLRKGEFESVLKTYAQIGLNVKGID
                     AREHFYTKLAGKTDPEEKRKIIGAGFIDVFQEEAKKVEGVGLLGQGTIYPDVIESVSV
                     HGPSVTIKSHHNVGGLPEHLHLELVEPLRSLFKDEVRKVGRELGIPAEMIDRHPFPGP
                     GLAIRILGDITEEKVQLLQQADHVYIKALKDNNLYATIWQAGAILLPVKSVGVMGDER
                     TYEYTVALRAVTSVDGMTADWAHLPYDFLAYVSNEIINNVRGINRVVYDISSKPPATI
                     EWE"
     gene            5180..5902
                     /locus_tag="PLU11_09075"
     CDS             5180..5902
                     /locus_tag="PLU11_09075"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPN59313.1"
                     /translation="MKRILVLLAATMLVFSSCSNFMGKRVRGNGKMVTETRSSGSFDG
                     VRVSGAIDLFVRTDSVTAVRVDGDENLLKYVEVELDGSMLRIRTRSGINLKPTRSIRV
                     YVSAPEFSRLEASGACDIFTENKINSTGTLNVDLSGASDAKLEVNAPGVKVEVSGAGS
                     VTLKGETRDFSVDGSGSTDIKCYELLAENTKVDISGAGNAEVFASVKLDVEVSGAADV
                     KYKGNATVSQRVSGAGSVKKIN"
     gene            complement(5992..>6377)
                     /locus_tag="PLU11_09080"
     CDS             complement(5992..>6377)
                     /locus_tag="PLU11_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59314.1"
                     /translation="VGNLSWNLKDQDLSNLFATHGEVSSAKIVTDKFTNRSKGFGFVE
                     MTNDDQAQAAIAALNGTEVDGRNIVVNESRPKPEGGGGGGFKKRSFGNGGGGGGYKGG
                     GGGGFKKGGGGGGFNRDRGGYGNDY"
BASE COUNT         1578 a         1513 c         1548 g         1738 t
ORIGIN      
        1 acttcctggt aggcctggcg cagcagcagg ttgtccgaat catattcact gaatacatta
       61 aataaaaggg aagcggagga ttgcaggtgc cgggccttct ttttttctcc tggcataccc
      121 tggaaaatca agccaccaat cacggccaca tccctgaatt tccgccgggc catctcggta
      181 ttatttacac tccgctggat atccaatagc aatccatcag tggtaaataa atcatacaca
      241 ttggtgtcat ctaccgggat aggctgatca ctcagcagtt caaaaccata atcattcatg
      301 gcaaaggaaa acgtgattgg ctgaatccgg ctgatccgcc aggccagtat tgccgccata
      361 gcttcatgta cgaggcgtcc ttcaaaaggg tatacaaaaa gatggaatcc atcttttgtt
      421 tcaatctgct cgatcagcaa ttcattttca gcaggtacat gcgaaagggc agcctgcaat
      481 tcaaatagcg ggtgcagggc tttgagttct tccgtttcag ttgaagttcc ctggcggtac
      541 ctgtttagcg ctgcacgaag tttcttcccc aggttagcgc taagtggcat ccgccctcca
      601 ttccagctgg ggataatgga ttttttactg gatgattttc gtaccaggac tgtcatgtct
      661 ttgatcatga caaattccag gtttcggcct gccagcgtaa atacatcccc aggtgataag
      721 cgggaaataa agtactcttc aattacccca atataccctc cggatatgaa ttttactttc
      781 agcatggcat cacttacaat ggtgccgata tgcatccggt gtcgcatcgc cagcttccgg
      841 ctctttatcc ggtaaacgcc gtcttccagt tccacttttt taaaatcatc atattgttgc
      901 atagcctttc caccctgggt ggcaaaatgg agtaattgcc cccattcttc ttccgtcagg
      961 tcacggtaac aataggtggt cctgatttca tgatataaag aaggatgggt gaatccttcc
     1021 gaaatggcca gggtattcag gtattgcagc aacacatcat agcaaccggt gaaagggatc
     1081 cggctctcaa tcagattttc atcaatggcc gatttaagtg cggccgcttc ggtcagttca
     1141 agcgagtgcg tgggcagaaa gtagatccgg ctgacggcat ccggctggtg cccgctacgg
     1201 ccggctcttt gtaaaaaccg ggctactccc ttgggggaac ccacctggat caccgtttcc
     1261 actggtcgga aatcaacccc caggtcaaga ctcgccgtgc aaaccacggc tttcagtttt
     1321 cccgtatgca aattatcttc aatccagttc cggagttcag gatcaatact tccatgatgc
     1381 agggcaatca caccagccag atcgggacag atgtccagca aggtctggta ccaggtttcg
     1441 ctcatacccc gggtattaat aaagatcagg gtggttccgc ttgcctgtat aatcggaatc
     1501 agtttgtgtg ccagcttgat tcccaggtga ccggcccagg gatacttttc aatttcatcc
     1561 ggatagatgg attctacctg gatttttttc tgaagggctg ccgttacggt tatgcatggc
     1621 tgttgcaatg gggagagcag cacctcaccg gcttcttcga gatttccaat tgtggcggat
     1681 ataccccata ccatgggccg ttgcgttgga ttttcctgca ggtgtaccag gcgggaaatg
     1741 gccagctcta cctgtactcc ccgtttgctg ccgagtaatt catgccattc atccaccgca
     1801 ataaccgcca gcgactgaaa gagagcaggg tatcctttct gcgccagtaa taaatgaagg
     1861 ctttccggcg tgatgatcag gatctccggc atttgttttt tttgccgggc cctttccgct
     1921 atcgaagtat ctccattccg tataccgact ttccatggaa gctgaagttc cgtcaaaact
     1981 tcttccatcg cccttccgat atccttggcc agggcacgaa gcggggttac ccataataat
     2041 tgtaatccgg catttttccg ggttccgtaa tcgtcaggat gaagattgat atagcggatc
     2101 agcgtgccca ggaatacaga gtacgttttc ccacaaccgg taggcgcatt gacgagcccg
     2161 ctttttcttt gggagatatg ggtccaggtt tcttcctgga ataaaaatgg cctgaaactt
     2221 tttgcttcaa gccatttgta aattaattta tatccgggtg tggcatgcac gtgtgggatc
     2281 gtattgttca gcagggggct aattcaccga tttaatgatt ccatcctgcc atttcagaaa
     2341 aaagagtttc ttgttttcaa aataatccaa cgtcatggtt tgtttattca acaccggttg
     2401 aatatccatt tcccggaata cattgaattc tttccgggtg atgttggagg aaaaatcagc
     2461 tttatacagc agcatcagtt ttacaaaacg ccagactgtt tcatatcccc gcatgaccat
     2521 atcactgggt cgggcaaaca tccgggtatt aaaagtattg gtgatactct ggcttacttt
     2581 atccgtacgt ggattataaa aaggagtact gtatacaatt tcaattcccc tgtattcggg
     2641 gcgggtaaaa tcctttattg catcccaggt aggcattccc atcaccgtgg ctttcaggtt
     2701 ttgttttttt aagacagcca actgaagggc cagccttttg ccgaaatttt catccaggct
     2761 gccggcaata cataaggtgt tttgaagggt atcaagataa ggggagagct gattgacctg
     2821 gaaactgtcg gtaagatcga tatactttaa tttcaaggga acgcccatcg tgtttttggc
     2881 aaactcatca aagtaagatt taatacggtc ttcaaggact ccttttttcc ggaatacaat
     2941 gagccggtcc agcgcataga acttttggat atgccggtag atcccttcgc attgagtctt
     3001 cagggtggaa ttgagcatca catagtacgg attaccggta accccaccgt cattgggcag
     3061 gttgacattg atcaccggaa tgttcatttg taatcccgca tcggcaaacg actttacttc
     3121 atttgctgag caataggcga tgagcaggtt aatatcctta actgacgcat tggccagttg
     3181 ctgttgtacg gtttggtgga cagatcgggt atcgaatacc tgcacatcga ggtttatttt
     3241 ttccctgttc agtgaatcca gggccagctg ggcgccttca taaaactcca gtcccggatt
     3301 aataaacttg ggaaatgtat ttttcgcata gcggtattcg gactggtcgt cgaaagcaga
     3361 gtccaggtac aacggtgcga agatggcaat cttaaatttt ggtgcaggtt cctgtgcccc
     3421 ggcggtttgt accaggaaaa acgacaggaa aagcacccag gtttgaatct tcatatagta
     3481 gatagttatt cccattctat tgttgcagga ggtttactgc tgatatcata aaccacccgg
     3541 ttgatacccc gtacattgtt tatgatttcg ttggacacat aggccaggaa atcataaggg
     3601 agatgcgccc agtcagcggt catgccatcc acactggtta ccgcccttaa tgcaaccgtg
     3661 tactcatagg ttctttcgtc gcccattacg cccacgcttt ttacaggaag taagatagcg
     3721 ccagcctgcc agatcgttgc atagagattg ttatctttca gtgctttgat gtacacgtga
     3781 tctgcctgtt gcaataactg taccttttct tctgttatat cgcccaggat acggatcgcc
     3841 aggcccggcc cggggaaggg gtgccggtca atcatttctg ccggtatgcc cagttcgcgg
     3901 cctactttac gtacctcatc tttgaaaagg gagcgcaggg gttcaaccag ttcaaggtgc
     3961 aggtgctccg gtaaaccacc cacattatga tgcgatttga tggtaacgga aggtccatgt
     4021 acggaaacac tttcgatcac atccggataa atagtgcctt gtccgagtaa acccacccct
     4081 tccactttct ttgcttcttc ctggaaaaca tcgataaatc cggccccgat aatttttctt
     4141 ttttcttccg gatcggtttt tccggccagc ttggtataga aatgttcccg ggcatcaatc
     4201 ccttttacat tcaggccgat ctgggcatag gtctttaata ccgattcaaa ttcgccttta
     4261 cgcaaaacac cattatcgac aaaaatgccg aaaaggcggt ccccgattgc ccggtggatc
     4321 agggtggcgg ctacagtaga gtcgaccccg ccgctaaggg ccatgattac tttccggtca
     4381 ccgatctgtt tttttaactg atcaaccgtg tcggtgataa aatgggcagg ggtccagtcc
     4441 tggctgcaac cacaaatctt taccaggaaa ttatggatga tctttttacc ttctttggag
     4501 tgataaactt ccggatggaa ctgtacgcca taaagtgggt ttccatcatt tccggctttg
     4561 cggaaagcgg caaccgggat actgtcggta tcggccagca gttcaaatcc ttccggcaat
     4621 tgtttaatgg tatcactgtg actcatccat acctggctct gatcgctgac ctcactgaaa
     4681 agggtgtctt cctttttacg ttgaaggatg gcacgtccgt attcgcgctt atcactttta
     4741 gccaccactc cgccgtaacg tttggcggtt aattgggcgc cgtagcagac gccaagaacg
     4801 ggaacctggc tgaccaggtt ggctacatcg gtatccggtg cattgggttc attcactgaa
     4861 aaaggggatc cggaaaggat gatgccttta aggcctggtt cgaatgtaaa gggtttatag
     4921 aacggaataa tttcacagta tacattggct tctctgacgg ctctggcaat caactgtgtg
     4981 tactgtgaac cataatccag gatgagtatc ttttctgtca tagtgctgcg aaggtaaaga
     5041 aaaccggaca ggctggaagc cgtcggctgc cggtaaaatg ggaaaaatgc cgcttgtggg
     5101 taaccttctt ttaagtccgg cgtctaactg gaaaagcagt caaaccttgg tatctttggt
     5161 taacttaaat agcacagtta tgaaacgtat tttagtcttg ctggccgcca ctatgctggt
     5221 gttttcatcc tgcagcaatt ttatgggaaa acgcgtccgg ggaaacggta aaatggtgac
     5281 ggaaacccgg tcctccggat ctttcgacgg agtgagagtg agcggggcaa tcgatctgtt
     5341 tgtaagaaca gacagtgtta cagccgtacg agtcgacggc gatgaaaacc tgttgaaata
     5401 tgtggaagtg gagctggacg gatccatgct ccggattcgc accaggagcg gaatcaatct
     5461 gaagcccacc cgatcgatcc gggtatatgt gagtgcccct gaattttccc gcttagaggc
     5521 atccggggcc tgtgatattt ttacggagaa taagatcaat agtaccggaa cattgaatgt
     5581 ggatctgagc ggggcatcgg atgcaaaact ggaagtgaat gcgccggggg taaaagtaga
     5641 agtaagcggg gccggttctg taaccctgaa aggagagacc cgtgatttca gtgtggatgg
     5701 ttctggaagt acggatatca agtgttatga attgctggct gagaatacga aagtggatat
     5761 ttccggcgca ggaaatgcgg aagtatttgc cagtgtaaaa ctggatgtgg aagtatcagg
     5821 agccgccgat gtgaagtata agggcaatgc aacagtgagt cagcgggtat ccggtgccgg
     5881 tagtgtaaag aagatcaatt aaagtacagt aattcacagt acggtgcaga taaaaaaagt
     5941 ctggttcgac aatgtcggac cagactttca atttattggg aagacttacg attaatagtc
     6001 gttgccgtat ccaccgcgat cacggttgaa accgcctccg ccgccgcctt tcttaaagcc
     6061 gccgccgcca ccacctttgt agccgccacc gccgccacca ttgccaaagc ttcttttctt
     6121 gaagccgccg ccgccgccac cttccggttt cggacgactt tcattaacaa caatgttacg
     6181 accgtcaact tcagtaccat tcagggcagc aattgcagcc tgagcctggt cgtcattggt
     6241 catttctaca aatccaaaac ctttgctgcg gttagtgaat ttgtcggtaa caatttttgc
     6301 ggagctaact tcgccatgtg tggcaaaaag gttagataaa tcctgatcct tcaggttcca
     6361 actgagattt cctacgt
//
LOCUS       DAOPMW010000173         6361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14046_length_6361_cov_12.759753, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000173 DAOPMW010000000
VERSION     DAOPMW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14046_length_6361_cov_12.759753"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLU11_09085"
     CDS             <1..654
                     /locus_tag="PLU11_09085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPN59315.1"
                     /translation="LMIINVLVFLTQNIVGQREEGLVENWFALHDLHSEFFKPHQLIT
                     HMFMHGSFAHLFFNMFGLWMFGAAIEHRYGAKRFLQFYIISGLGAAVLHMGILYLEME
                     PIMDIFRRLPMEEQLELIESPSFKVNVATLGASGAVFGCLAAFGYLYPNSIIMINFLL
                     PMKAKWLVLIYAGMELFLAFRNSAGDTVAHFAHLGGALTGFLLVYFWNKRNRRTFYR"
     gene            696..1637
                     /locus_tag="PLU11_09090"
     CDS             696..1637
                     /locus_tag="PLU11_09090"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPN59316.1"
                     /translation="MTYTEPSYRQRITFGQSNNAVTRLIVINLVVFISIALTKVFYYY
                     TYRTEAEVDLHFNAEVIPLAVLPASLTDLLYKPWTVLTSMFTHIGVWMTLANMLWLWT
                     FGYIMQDLTGNRKIVPLYIYGGLAGVIGFLLAANLLPANAAHPAASIYYGGAASITAI
                     AVATTVVSPGYRIFPLLNGGFPLWILTAFYLLISIATLPFYSVTAYVPPVAGALTGYL
                     FIWFIRRGYDWSEWMSNFFDWINDLFNPDKPRKGQPPIKDQLFYKTGTTPYTKTPHVT
                     AERIDAILDKINQGGYNSLTAEEKDILRRAGEDDVRI"
     gene            complement(1638..2216)
                     /locus_tag="PLU11_09095"
     CDS             complement(1638..2216)
                     /locus_tag="PLU11_09095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPN59317.1"
                     /translation="MFNLILFGPPGSGKGTQSDKLVEKYGLKHLSTGNLLRQEIADKT
                     PLGLEAKSFIDKGQLVPDEVVIGMVDSFFELHKDAKGFLFDGFPRTAAQAVALDKLLD
                     LKETGIAAVLALEVGEEELVKRLLNRGITSGRSDDTNEEVIRKRFAVYSTETSAVAEH
                     YKAAHKFKSIKGEGSVDAIFDSLCAAIDKKMN"
     gene            2395..2973
                     /gene="msrB"
                     /locus_tag="PLU11_09100"
     CDS             2395..2973
                     /gene="msrB"
                     /locus_tag="PLU11_09100"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPN59318.1"
                     /translation="MKPIMRFSFLVLFCGLLQQCSYSQNNQSPAKTATMDSLKKNNPV
                     YSNSDTSKINLPEEEWKKILPADVYQIARQKGTERPWTSKFESFKEVGTYYCAACGNA
                     LFKSDTKFESGCGWPSFYEPISKGSIIYTPDNTHGMERTEVQCGRCKAHLGHVFDDGP
                     PPTGLRYCINGVILDFQKANEAAKKYKENTNQ"
     gene            3073..5913
                     /locus_tag="PLU11_09105"
     CDS             3073..5913
                     /locus_tag="PLU11_09105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPN59319.1"
                     /translation="MPTCSRLYNTLILLLLSVSLFAQINPQQPMPVDPKVKIGKLSNG
                     MTYYIRNNIKPEKRVELRLVVNTGSVLEDEDQLGLAHFMEHMNFNGTKRFPKNELVNY
                     LQSIGVEFGADLNAYTGFDETVYMLPVPTDKPGLVDKGLQILEDWAHNALLDSTEIEK
                     ERGVVIEEWRLSRGADERMLKQTLPVQYQGSRYAERLPIGKLETLQHFTHAALKRFYT
                     DWYRPDLQAIIVVGDVDVNDMEQKIREIFGDIPAAGTPRKRENFPVPDHDEVLTVVAK
                     DKETAFPSVEILYKKDPLPETTVGDYVRYMNGSLFTGMLNNRFRELTLQANPPFVGAG
                     SYYGESYARSKNAYQLFANTSDTGMRRSLYVLAEENRRILLHGFTPSEFDLQKKQILS
                     YYDRVFNEREKQESYNYVEEYIRNFLVDEPIPGIEWEYDFVKQYLASVTLPEVNQLAA
                     QWISNKNMVVTLNAPDKEGIKIPSAEEVKAVLSSVETAAISPYKEKILAKNLMDASQL
                     KGGKILRTKTDEEVGTTVLLLSNGATVVLKPTTFKNDEILLRAFSKGGHSLVKDADYY
                     SAIHSSAIINQSGVGAFSAIDLGNMLKGKNTSVSPNIGGLSEGLSGSTIPKETETMLQ
                     LLYLYFTAPRKDAAAFESFKTRQKQLYANVASNPDIFFSGEVQKILTSNHPRGGKIPT
                     PEDFDKINLDRSFQIYKERFANAGDFTFIFTGSFNEETIRPLLEKYIGSLPGSPKKEN
                     FRDLGIRPPQGKVDKTINKGSEPKSTVNIVFMTPAVYNGNEAYALKSLAELMDIKLVE
                     QLREEKSGVYGVSAGGSMNRIPYGNVSFSISFPCAPENADTLTKAALDELKKIMQKGV
                     SPEDLEKIKEQQKRKLEVDIKQNSFWANGLLEAYFYGADPAELLKKQQQIDKLTGKMM
                     QDVAKKYINLQRYIRVTLKPETEEAKKPLKGF"
     gene            5925..>6361
                     /locus_tag="PLU11_09110"
     CDS             5925..>6361
                     /locus_tag="PLU11_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN59320.1"
                     /translation="MKYLRLFIFFIVSLIVVVMVFSWLVPVKQSLDKSISIQAPAAVV
                     YEQVARLENFNQWSSWNQHDSTVKLEYTGTDGSVGASSHWTGDPKISGEGRITIDSLE
                     PGKMVSQKIHFIKPKEGHASSSFTLSELNGLTTVSWHFESETPR"
BASE COUNT         1783 a         1521 c         1468 g         1589 t
ORIGIN      
        1 ctgatgatta tcaatgtgtt ggtgttcctt acccaaaata ttgtaggtca acgggaagag
       61 gggcttgtgg agaactggtt tgccctgcac gacctccact cggaattttt caagccgcac
      121 cagctgatca cgcatatgtt tatgcatggc agtttcgccc acctgttttt caatatgttc
      181 ggtctctgga tgttcggggc ggccatcgaa caccgttatg gggccaaacg atttctccag
      241 ttttatatca tttcggggct gggtgccgct gtattacata tgggaatatt gtacctggaa
      301 atggagccta ttatggatat ttttcgtcgg ttgccgatgg aagaacaact ggaactgatt
      361 gaaagccctt ctttcaaagt taatgttgcc acccttggtg cctccggtgc ggtatttggc
      421 tgtctggccg cttttggcta cctgtacccg aacagtatca tcatgatcaa cttcctgctt
      481 cccatgaaag ccaaatggct ggtactgatc tatgccggta tggagctatt cctggctttc
      541 cggaactcag ccggtgatac cgtagcccat ttcgcccacc tgggcggggc cctgaccggt
      601 tttttattgg tgtatttctg gaacaaaaga aaccgccgca cgttttacag gtaaaatgac
      661 ttactcccaa cccgtacctt tgccagcgtt acattatgac ttacactgag ccttcataca
      721 gacaacgaat cactttcggg cagagcaata atgcggtcac ccgcctcatt gtaattaacc
      781 tggtggtctt tatttccatc gccctgacca aagtcttcta ttattacact taccgcaccg
      841 aggcggaagt ggaccttcat ttcaacgcag aagtaatacc attggctgtt ttaccggcca
      901 gccttacgga tctcttatac aaaccctgga ctgtgcttac gtccatgttc acccatatcg
      961 gggtctggat gaccctggct aatatgctct ggctctggac gttcggttat atcatgcagg
     1021 acctgaccgg taaccggaaa attgttccct tatatattta cggcggatta gcaggtgtga
     1081 ttggcttttt actggcggcc aatctgctcc cggccaacgc agctcatcct gcagccagta
     1141 tttattatgg aggagccgcc agcatcaccg ccattgccgt tgccactacc gtagtatccc
     1201 ccgggtaccg cattttccca ctgctgaacg gcggatttcc gctctggata ctgaccgctt
     1261 tttacctgct gatttctatt gccaccctgc ctttttatag tgtaaccgcc tatgtgccgc
     1321 cggtggccgg ggcgttaacc gggtacctct ttatctggtt tattcgcagg ggatatgact
     1381 ggagtgagtg gatgagtaat ttcttcgatt ggataaatga tctctttaac ccggataaac
     1441 cccggaaggg acagccgccg ataaaagacc agttgtttta caaaaccggc actacgcctt
     1501 atactaaaac gccacatgta acagccgaac ggattgatgc cattctggat aagatcaacc
     1561 agggcggata caattcactg actgccgaag aaaaagatat tctgcggcgg gccggcgaag
     1621 atgatgtacg aatctgatca gttcattttc ttatcgatcg cagcacaaag cgaatcgaaa
     1681 atggcatcca cagatccttc tccttttatt gatttaaatt tatgcgccgc cttataatgt
     1741 tcggctaccg cagaagtttc ggtgctatac acggcaaagc gtttgcggat cacttcctca
     1801 ttggtatcat cactgcggcc agaggtaatg cccctgttca gcaggcgttt taccagttcc
     1861 tcctccccta cttccagggc gagaacggcc gcaataccgg tttcttttaa gtcgagtaat
     1921 ttatcgagtg ctacagcctg ggcagctgtg cggggaaagc cgtcaaataa aaatcctttt
     1981 gcatccttgt gcagctcaaa gaaagaatcc accataccaa tcaccacttc atcaggcacc
     2041 agctgccctt tgtcaataaa actttttgct tccagtccga gcggggtttt gtccgcaatt
     2101 tcctggcgga gcaggttacc ggttgacaaa tgtttcaacc cgtatttttc gaccagtttg
     2161 tcggattggg tgcctttacc actgcccgga gggccaaaga gaattaagtt aaacataatc
     2221 agatagctat gttggtgtat ggacaaatat aacatacaag cactgaaaat cgcctgtcat
     2281 cgctcacttt taaagaggat tcttatttgc catcaaaatg ttaacaaatg tcagtagttc
     2341 ccgatgccgg aaaccggcca aaccttgttt gcgtatgttt gttaaagaat gaatatgaaa
     2401 ccaatcatgc gcttcagctt cctcgtcctg ttctgcgggc tattgcagca atgcagctat
     2461 tcgcagaaca atcaatcacc cgcaaaaaca gcaaccatgg acagccttaa aaaaaataac
     2521 cctgtgtatt ccaattccga tacctcaaaa atcaatctgc ctgaagaaga atggaaaaaa
     2581 atattaccgg cggatgtata ccagattgcc cggcagaaag gcacggaacg tccctggacc
     2641 agtaagtttg aaagttttaa ggaagtgggc acttattact gtgccgcctg tggcaatgcc
     2701 ctgtttaaaa gtgatactaa atttgaaagc ggctgcggat ggcccagttt ttacgaaccg
     2761 atcagcaaag gaagtatcat ttataccccc gataataccc atggtatgga acggacagaa
     2821 gtacagtgtg gacgctgtaa agcccacctg ggtcatgtgt ttgatgatgg tcctcctccc
     2881 accggccttc gctattgcat caatggggtg atccttgatt ttcaaaaagc aaacgaagcc
     2941 gcaaaaaaat ataaagaaaa tacgaatcaa taataaaata aactaataag taactatccc
     3001 ggcaaccact tgccgggatt tttagtttta aaagcggcga tgtaataatt ttacttatta
     3061 aatgttcctt gtatgcctac ctgctcccgc ttatacaaca cccttatcct gcttcttctc
     3121 agtgtctccc tttttgctca gatcaacccg caacaaccga tgccggttga ccccaaggtc
     3181 aaaatcggaa aactgtctaa tggaatgacc tattatatcc ggaacaacat caaaccggaa
     3241 aaaagggtgg aactacggct ggtggtcaat accggttccg tattggaaga tgaggatcag
     3301 ctgggactgg cccattttat ggaacacatg aatttcaacg gcaccaaacg gttccccaaa
     3361 aacgaactgg taaactacct gcaaagtatc ggggtggaat tcggggccga cctgaatgcc
     3421 tataccggtt ttgatgaaac cgtgtatatg ttgcccgtac caacagacaa acccggactc
     3481 gtggataaag gattacagat tctggaggac tgggcgcata atgccctgct tgattccact
     3541 gaaattgaaa aagaaagagg agtggtgatt gaagaatggc gactcagccg cggggcagat
     3601 gagcgtatgt taaaacaaac cctgccggtc cagtaccagg gttcccggta tgcggagcgg
     3661 ctcccgatcg ggaaactcga aacgctgcaa catttcaccc atgctgcgtt gaagcggttt
     3721 tataccgatt ggtaccggcc cgatctgcag gctatcattg tggtaggcga cgtagatgta
     3781 aatgatatgg aacaaaagat ccgtgaaatt tttggtgata ttcctgccgc cggcacaccc
     3841 cgtaagcggg aaaacttccc ggtgccggat catgatgaag tactgacggt agtggccaag
     3901 gacaaggaaa ccgccttccc ttctgtggaa atcctatata aaaaagatcc gctgccggaa
     3961 accaccgtgg gcgattatgt acgttatatg aacggttcat tatttaccgg catgctcaat
     4021 aaccggttcc gcgaactgac cttgcaggcc aatccgccct ttgtgggtgc cggatcctat
     4081 tatggcgaaa gttatgcccg gtccaaaaac gcataccagt tgtttgccaa taccagtgat
     4141 acaggtatgc gccgttcact ttatgtatta gcggaagaaa acaggcgtat cctcctgcat
     4201 ggtttcaccc cctctgaatt tgacttgcag aaaaaacaga tcctgagtta ttatgaccgc
     4261 gtattcaatg aacgggaaaa acaggaatcc tataattatg tggaagagta tatccgcaat
     4321 tttctggtgg atgaaccaat tcccggtatc gaatgggaat atgattttgt aaaacaatat
     4381 ctcgcttcgg taacactccc ggaagtcaac caactggccg cccaatggat cagtaataaa
     4441 aatatggtgg tcacgctgaa tgccccggat aaagaaggca tcaaaatacc atctgcagaa
     4501 gaagtaaaag ccgtgcttag ctcggtggaa acggcagcaa tcagtccgta taaagaaaaa
     4561 atactggcaa agaacctgat ggatgccagc cagctcaaag gcgggaagat cctgcggacg
     4621 aaaacagatg aagaagtagg cactactgta ttgcttttat caaatggagc caccgtggtg
     4681 ctgaaaccga caacctttaa gaatgatgaa atcctgttaa gggcattcag taagggcggt
     4741 cattccctgg tcaaagacgc ggattattat tcggccatcc attcctccgc tattatcaat
     4801 cagagtggtg tgggtgcctt ctcggcgatc gacctgggta atatgctgaa aggaaaaaac
     4861 acatccgtat cacccaatat cggcggatta agcgagggcc tttccggcag tacgattccg
     4921 aaagaaaccg aaaccatgct gcagctcctc tacctctatt ttaccgcacc aagaaaagat
     4981 gccgcagcgt ttgagtcctt taaaacaagg cagaaacaac tctatgcaaa tgtggcgtcc
     5041 aaccctgata ttttcttcag cggagaagta caaaaaatac tgaccagcaa tcatccccgt
     5101 ggcggaaaaa ttccgacccc ggaagatttt gataagatca acctggaccg tagttttcag
     5161 atctataaag aacgttttgc caatgccggt gattttacct tcatcttcac cggttcattt
     5221 aatgaagaaa caatccgtcc attactggag aaatatatcg gtagcctgcc cggctcgcca
     5281 aaaaaggaaa atttccgtga cctcggcatc cgtccgccac agggcaaagt agataaaaca
     5341 ataaataaag gcagcgaacc caagagtacg gtgaatattg tatttatgac tcccgcggtc
     5401 tataacggaa atgaagccta tgcactaaag agcctggcag aactgatgga tattaaactg
     5461 gtagagcagc ttcgtgagga aaaaagcggg gtatacggcg tgagtgccgg cggatccatg
     5521 aatcgtattc cgtatggcaa tgtcagtttc agtatcagtt ttccctgcgc cccggaaaat
     5581 gcagataccc tgacaaaggc cgcactggat gaattgaaaa aaatcatgca gaaaggcgtc
     5641 agcccggaag acctggagaa aatcaaagaa caacagaagc gaaaactgga agtggatatc
     5701 aaacaaaaca gtttttgggc caacgggttg ctggaagctt atttctatgg tgctgatcct
     5761 gctgaactgc tgaagaagca gcagcagatc gataaactga cggggaaaat gatgcaggac
     5821 gtagcaaaga aatatatcaa cctgcaacgc tatattcgcg taacgctgaa acccgaaacg
     5881 gaagaagcca aaaaaccatt gaaaggattc tgatactata acatatgaaa tacctgcgcc
     5941 tgtttatttt ctttattgtc tccctgattg tggtcgttat ggtgttttcc tggttagtcc
     6001 cggtaaaaca atcactcgat aaaagcatca gcatccaggc gcctgcagcc gtagtgtatg
     6061 aacaggtggc ccggcttgaa aacttcaacc aatggtcgtc ctggaaccag catgactcca
     6121 cggtaaaact ggaatacaca ggaacagacg gcagcgtggg agcgtccagt cactggaccg
     6181 gtgatcccaa aatttcagga gaaggccgca taacaatcga ctcactcgaa ccgggtaaaa
     6241 tggtcagcca gaaaatacat tttatcaaac caaaagaggg acatgcttct tcgtccttta
     6301 ccctcagtga gctgaacgga ctcactacgg tcagctggca ttttgaaagt gaaacacccc
     6361 g
//
LOCUS       DAOPMW010000174         6354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14061_length_6354_cov_4.611049, whole genome shotgun sequence.
ACCESSION   DAOPMW010000174 DAOPMW010000000
VERSION     DAOPMW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14061_length_6354_cov_4.611049"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLU11_09115"
     CDS             <1..272
                     /locus_tag="PLU11_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59321.1"
                     /translation="FFRIFQESITNVIRHSDATTVLVELSRLGGHISLKITDNGKGFD
                     MSTVSGKRTLGLLGMRERSLLIGGECRIESIPGRGTTVSVEVNPN"
     gene            complement(303..641)
                     /locus_tag="PLU11_09120"
     CDS             complement(303..641)
                     /locus_tag="PLU11_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59322.1"
                     /translation="MNTSANNEFLQLVSSLHKEGYNLDQITLQLKEKGAPENFFQEAI
                     SHLKQLKLNRKRSTGFACCGIGIALLVVGCMLTVLLYNSGGDIKLAMYGLTTLGVIFA
                     MKGLVDILGW"
     gene            complement(705..1760)
                     /locus_tag="PLU11_09125"
     CDS             complement(705..1760)
                     /locus_tag="PLU11_09125"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPN59323.1"
                     /translation="MKILLIALTGLLLCASCTNNDQGKGKDGDNQATEYVFSSGLTED
                     TACLLSQVAYCVNIDSMLRGYMPGWSSIWEGKESNGNHAFVASNGRDFALAIRGSLIN
                     FSWAAFQNWIYQDLYVTTQENWPFAPDSLQARVSAGSYTGFQNLSAMTDLKDGRLLLP
                     VLDSILNTGAPLLITGHSLGGNLATVYGSYLAATRQKKGKDKDPVNVITFAAPAAGNA
                     GFAADFNKKFPQSIRVENRNDIVPKFPSGSRIASLGELYSDSLSAANIQVGYKNLQLP
                     LSRVFSLLKSGLTLLEFTSGLSPYEQTNGAGKQISIPLSGKNPGHEIINWLAEAGYQH
                     RVGTYAVYEKVPVLPCD"
     gene            2003..3142
                     /locus_tag="PLU11_09130"
     CDS             2003..3142
                     /locus_tag="PLU11_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59324.1"
                     /translation="MKKIYSIIVLALLAASVNVNAQSFPWSSHSKGSKSSVYSQGALS
                     RNFNLTGNGTESDYPQYNNGGGGNLATSVNWSNRTSSVTMTITFSKPLVGVTFLLFDV
                     DQTTGSWDDKLTITALNETGATIYPALTGNSYTTISGTNRNVIEGKSNNTNYTNSPAV
                     ASFTSQYVKSLSIVFSAGSSSPSNPASQVVGVGGIVYENVLPIDLMWFKADKKNNTTD
                     LKWQTENQEGFSHFEIERSANGADDFTSIAKIQTSAATTGNYSYTDVNAARLSQKAYY
                     RLKMVDQDGQYKYSAVVVVTFENAASIMVTPNLLNAGEMISVNIAGNNQSKYDVKLFD
                     MSGKMISQQAGNSRMQIATSGLRKGMYIVSVSNLNEAKTFRIMVQ"
     gene            3585..3998
                     /locus_tag="PLU11_09135"
     CDS             3585..3998
                     /locus_tag="PLU11_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59325.1"
                     /translation="MKKIFTLVAALFLTVALFAADRRPVIKLNNNNNYKVVIDGRAYF
                     GDDINIHPDQFLRGKHTVKVFEMKRGFFGRTERLVDVTTFFIDRDDVVIRIDRMGRIL
                     VREERKGNWNNGNGKNQRDRNDDRYDRNDRPVRRF"
     gene            complement(4221..4775)
                     /locus_tag="PLU11_09140"
     CDS             complement(4221..4775)
                     /locus_tag="PLU11_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPN59326.1"
                     /translation="MTLHTDLLIEKKSVLPAGDFEGCRFIHCDLGGADLSGINFTESV
                     FEDCNLSGCKILQTTFNDARFVRCKMLGLHFESASQYLFTVRFEYSTLDLCSFYQCKM
                     KKTSFSQCSLQEVDFTETLLTEAVFDGCNLRDAKFENTVLEKADLRTAVNFTINPEIN
                     KIRKARFSYPAVLSLLDKYGIIIS"
     gene            complement(4867..5310)
                     /gene="tnpA"
                     /locus_tag="PLU11_09145"
     CDS             complement(4867..5310)
                     /gene="tnpA"
                     /locus_tag="PLU11_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPN59327.1"
                     /translation="MIYLLPRIWIHAIWSTKDNIPFLNQSIEKSVHFYLSSKFEEAGC
                     QVRIINGMPDHVHCLFQQNPLKSVSDVLKYVKSGSSYWINNQGMLKERFTWQKGYAAY
                     SVGSTGLEWVQHYIAEQKNNHRKKTFQEEFNHFTRMHGCDEAKLR"
     gene            5486..>6354
                     /locus_tag="PLU11_09150"
     CDS             5486..>6354
                     /locus_tag="PLU11_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPN59328.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLQSTGTISDRDMMDQVLDDM
                     DLEREKGITIKSHAIQINYKHTDGQEYILNLIDTPGHVDFSYEVSRALAACEGCLLLV
                     DATQGIQAQTISNLYLAIENNLEIIPVINKIDMDGAMIEEVEDQIVDLIGCKREEILH
                     ASGRTGLGVEKVLDAIVSRIPAPTGDPDAPLQALIFDSVFNSFRGIIVYYRIMNGSIK
                     KNDKVKFVSTGQEYGADEIGILKLKMTEKNVVKAGDVGYIITGIKNAKEVKVGDTITV
                     ATNTNPQQIQGFE"
BASE COUNT         1800 a         1423 c         1414 g         1717 t
ORIGIN      
        1 ccttcttccg tattttccag gaatccatta ccaatgtaat caggcattcg gatgccacta
       61 ccgtattggt ggaactcagc cggctggggg ggcatatcag tttgaagatc accgataacg
      121 gaaaaggctt tgatatgtct accgtatccg gtaagcgtac acttggcctg ctgggtatgc
      181 gggaacgttc actgcttatc gggggcgaat gccgtattga gagtattcca ggcaggggca
      241 caacggtatc tgttgaggtt aaccccaact gatattagtc atgcgaataa tcaagatgat
      301 ttttaccagc ctaaaatatc caccagtcct ttcatggcga agataacacc aagggtggtg
      361 agaccataca tcgccagttt gatatccccc ccgctgttat agaggagcac agtgagcata
      421 catcccacta ccaataaagc gatcccgatt ccgcagcagg caaagcctgt gcttcttttg
      481 cggtttaact ttaattgttt aaggtgactg atggcttcct gaaagaaatt ctcaggagcg
      541 cccttctctt ttaactgaag ggtgatctgg tccaaattat acccttcttt atgaagagaa
      601 ctgaccagtt gcaggaattc attgttggca gaggtgttca taaattggca agctttgaag
      661 taaattacgg aattccccga aattgccaaa aaatcgataa agtcttaatc acagggcaaa
      721 acaggcactt tctcatatac cgcgtaggtg cctacacgat gctggtaacc agcttccgcc
      781 agccagttga ttatttcatg tcccggattc tttccggata atggaatact gatttgcttc
      841 cctgcgccat ttgtctgttc ataaggactg agcccgctgg taaattccag caacgttaaa
      901 cctgatttta aaaggctgaa aacccgggac aatggtagtt gcagattttt ataccccacc
      961 tgtatattgg cagctgacaa tgaatcactg tataattcac ccagggaggc gatacggctg
     1021 ccggaaggga attttggtac gatatcattc cggttttcca cccggattga ttgcgggaac
     1081 tttttattaa aatcagcagc aaaccccgca ttacctgcag caggggcggc aaacgtaata
     1141 acattgaccg ggtctttgtc ttttcccttt ttttggcggg tggctgccag gtaggatccg
     1201 taaacggtag ccagattccc acccaggctg tgaccagtaa tcaggagcgg ggctccggta
     1261 ttcaggatgg aatcaagtac gggtaataat aatcttccat cttttaaatc tgtcattgcg
     1321 gataggttct gaaatccggt atatgaaccg gcagatacac gcgcctgcag actatcaggt
     1381 gcaaagggcc agttttcctg agttgtgaca tacagatcct ggtaaatcca gttctgaaag
     1441 gctgcccagc tgaaatttat taaagatccc ctgatcgcca acgcgaaatc gcggccatta
     1501 cttgctacaa atgcgtgatt accatttgat tcctttcctt cccatatgga agaccagcca
     1561 ggcatatacc cgcggagcat actatctata ttaacacaat aggctacttg agaaaggagg
     1621 caggcggtat cttctgttaa gccggaactg aaaacatatt cagtggcttg attatcacca
     1681 tcctttcctt ttccctggtc attgtttgta caggaggcgc atagtaataa tccggttaat
     1741 gcaatcagca gaattttcat attgatttct ttccgcctaa aaataagtgt tccttccatt
     1801 tcgataattg aatttttaac ccttagatag actcgtttag ctcattttac tcttaacagg
     1861 cataagggga tacctataaa atagaccatt tttccatttt ctggacagtc ggccacattt
     1921 aagtttcgta aataactgaa aatcaagtac gtaatttttg gcattggaat ggtaattata
     1981 cagtatccta aaatcgtact ttatgaaaaa aatttactca attatcgtcc tcgccctctt
     2041 agcagcatct gttaacgtaa acgcccagag ttttccctgg agcagtcatt caaaaggaag
     2101 caagtcttca gtttacagcc agggtgcact ttcccggaat ttcaatttaa caggtaatgg
     2161 tacagaatct gattaccccc agtataacaa tggtggtggc ggtaacctgg ccacttcggt
     2221 aaactggtca aacagaacta gttctgtaac catgaccatt acattctcca aaccattggt
     2281 tggagtgacc tttttgttat ttgatgtgga tcagacaaca ggtagttggg atgacaaact
     2341 gaccattacg gccttaaatg aaacaggggc tactatttac cctgccctga ccggtaattc
     2401 ctataccacg atctctggaa caaacagaaa tgtgatcgaa ggcaaatcaa acaatacaaa
     2461 ttataccaat tcccctgcgg tggctagttt tacaagccag tatgttaaat cactcagcat
     2521 tgttttctct gccggttcat ccagccccag caatcccgct tcacaggttg tgggtgttgg
     2581 cggaattgtg tatgagaatg tactgccgat cgacctgatg tggtttaaag ccgacaagaa
     2641 aaataacacc accgacctga aatggcagac tgaaaaccag gaaggattca gccattttga
     2701 aattgagcgt tctgccaatg gggctgatga ttttacttct attgcaaaga ttcagacttc
     2761 tgcagctact accggtaact acagctatac agatgtaaat gcggcccgcc tttctcaaaa
     2821 agcttactac cgtcttaaaa tggtggatca ggatggtcag tataaataca gtgcagtagt
     2881 ggtggtgact tttgaaaatg ccgcttcaat catggttact cccaatctgc tgaacgccgg
     2941 tgaaatgatc agtgtgaaca ttgccggaaa taatcagtca aaatacgatg tgaagttgtt
     3001 tgatatgagc ggaaagatga ttagtcagca ggccggcaac agccgtatgc agatcgccac
     3061 ttcaggatta cgcaaaggca tgtacattgt aagtgtcagc aacctgaatg aagccaaaac
     3121 tttccgtatc atggttcaat aagttttcag gagtacgatt ctggatataa gaacactgag
     3181 cattaagtaa aagctgcttc cggaaggagg cagctttttt ttattcagtt gtcagttgtc
     3241 agttgtcaga tgtcagatgt caggaaacag cccctcctta gatatcgata aactctgcat
     3301 accatttaaa cttatcagtc tatcggtagt cggcagtaag cagtaagcgt cagcagctgg
     3361 cagtttctcc tatgaaaact gaatctaaaa caacaagctg acactcgttt tacgagcatc
     3421 tatcgtactt cgtacttcta acttcgtact tactcttaac atttcttttt cccttttccg
     3481 gcacactggc atctgctttg aattagtaaa cataaccgga caataaaaca aattgtactg
     3541 aaggtggtcc ggcatttcag tactgattat taaaacaaaa tatcatgaaa aaaattttta
     3601 ctcttgttgc cgccctgttt ctgaccgtgg ctttatttgc tgctgaccgc agacccgtta
     3661 ttaagttgaa taacaacaat aactataaag ttgtcattga tgggcgagct tatttcgggg
     3721 atgatataaa tatccacccc gatcaattcc tgcgcgggaa acacacggtg aaagtatttg
     3781 aaatgaaacg aggtttcttt ggccgcaccg aacgcctggt ggatgtcacc accttcttta
     3841 ttgaccggga tgatgtagtg atccgtattg acagaatggg caggatcctc gtccgcgaag
     3901 aaagaaaggg caattggaat aacggcaatg gcaagaacca gcgtgatcgt aatgatgacc
     3961 gttacgaccg taacgaccgt cctgttcgca gattctgatt actgaacaca aactaaaagc
     4021 gaaagccacc tggaatacgg gtggcttttt taatacaggt atgagctacg aggtacgagg
     4081 tacagtttcg aggtatgagg tatgagctgc gagcgacttt tcgtaaaacg agagacgatt
     4141 tgttaattta gtcaacattc ttaggagggg ctgtttccta acacctgaca actaacacca
     4201 aacacctgtg ttgactctcc tcagctgatt ataatgccat atttatcaag gagggacaat
     4261 acggctggat aggaaaaccg ggctttccgg attttattga tttcgggatt aatcgtgaag
     4321 ttaacggcag tccgcagatc cgctttttcg agcaccgtgt tttcaaattt ggcatcccgc
     4381 aggttgcagc catcaaaaac ggcttcagtc agcagcgtct ccgtaaaatc cacttcctgc
     4441 agactgcatt gactgaacga tgtcttcttc attttacatt ggtaaaaaga gcagagatca
     4501 agtgtcgaat actcaaaacg aacagtaaac aaatactgac tggctgattc gaaatgaagt
     4561 ccaagcattt tgcaccgcac aaaccgggca tcattgaatg tggtctgcag gattttacac
     4621 ccgctcaggt tgcaatcttc aaacacagat tctgtaaagt ttatgcccga caaatcagcc
     4681 ccgccaagat cacaatggat gaaccggcat ccttcaaagt cgccggcagg aagaacgctt
     4741 tttttttcga tcaacagatc tgtatgtaaa gtcatgcatt ttttttcagc aagcaatgta
     4801 agtaatctgc cgcaggctcc aaaaacaggc gatagggatg ttccggtttt cagtatagtc
     4861 acgaacttac ctcagcttcg cttcatcgca tccatgcatg cgggtaaagt ggttaaactc
     4921 ttcctggaat gtttttttcc ggtgattgtt tttttgttcc gcaatataat gctgcaccca
     4981 ctccagtccg gtagagccca ccgagtacgc cgcatagccc ttctgccagg taaagcgctc
     5041 ttttaacatg ccctggttat taatccagta cgaactcccg ctttttacat atttgagtac
     5101 atcagacacg gatttcagcg ggttttgctg gaacaaacaa tgtacatggt ccggcatccc
     5161 attgatgatc cggacctggc agcctgcttc ctcgaattta ctgctgaggt aaaaatgaac
     5221 ggatttttca atggattgat tcaggaacgg gatgttgtct ttggtggacc agatcgcatg
     5281 gatccagatc cggggtaata aatagatcat agtaaggttt tgacagctaa gctagcccgt
     5341 gtccggtgta cagaaagggg aaaacggccg gaaaaacccg cttttttccc cccgatggga
     5401 gctgttttca acccttgaat caactgcctt ttcctcgtcc aaatgctgta actttgcccc
     5461 ctcttaaaag gaggcaaaaa cctacatgaa gaatatccgg aatttctgca tcattgcgca
     5521 tattgaccac ggcaaatcca ccctggctga ccgattattg caaagcaccg gtaccatcag
     5581 tgaccgtgat atgatggacc aggtgttgga tgatatggac ctcgaaaggg aaaagggtat
     5641 tactattaaa agtcatgcca tccagattaa ctataaacac accgacggac aggagtatat
     5701 cctgaacctg atcgatacgc ccggtcacgt agacttcagt tacgaagtga gccgggccct
     5761 cgctgcctgc gaaggttgtc tcctgcttgt ggacgccacg cagggcatac aggcacagac
     5821 catcagtaat ctttatctcg ctatcgaaaa taacctcgag atcatccctg tcattaacaa
     5881 gatcgatatg gatggcgcga tgattgaaga agtggaagac cagattgtgg acctgattgg
     5941 ctgcaaaaga gaagagatcc tgcatgccag cggacgtacc ggactgggtg tagagaaagt
     6001 actggatgca attgtctccc gtattccggc accaaccggg gatccggatg cccccctgca
     6061 ggcgctgatt tttgactctg tattcaattc attccgcgga attattgttt actaccggat
     6121 catgaacggt tccatcaaaa agaacgataa agtaaaattt gtaagtaccg ggcaggaata
     6181 tggtgccgat gaaatcggta tcctgaaact gaaaatgacg gagaagaatg tggtgaaagc
     6241 aggcgatgta ggttatatca tcaccggtat caaaaatgcg aaagaagtaa aagttgggga
     6301 taccatcacc gtagccacca ataccaatcc ccagcagatc cagggatttg aaga
//
LOCUS       DAOPMW010000175         6337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14110_length_6337_cov_8.730977, whole genome shotgun sequence.
ACCESSION   DAOPMW010000175 DAOPMW010000000
VERSION     DAOPMW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14110_length_6337_cov_8.730977"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..633)
                     /locus_tag="PLU11_09155"
     CDS             complement(160..633)
                     /locus_tag="PLU11_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59329.1"
                     /translation="MKTFRATMLCVVLTLSAIVSNSQEQKIPLNEPDQNKPRLFSNLP
                     DKIPVTLTNIDNILSAPVGRNSQFRLSEDNTLQFAGEVVSTASKYNNSIQSVVIRSDD
                     FNGARLTISKITNPDGSIRYSGRIISFKHGDLYELENQDGQLVLVKKNYYELVNE"
     gene            complement(833..1198)
                     /locus_tag="PLU11_09160"
     CDS             complement(833..1198)
                     /locus_tag="PLU11_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPN59330.1"
                     /translation="MSQKKTLVLGASDNPSRYSYLAVQRLRSHGHPVLAIGRKATVVA
                     DVTVETAKHKYADIDTVTLYLNPQHQREYYDYILSLSPKRIIFNPGTENDELAALAAE
                     KNIQTIEACTLVMLSTNQY"
     gene            complement(1268..1666)
                     /locus_tag="PLU11_09165"
     CDS             complement(1268..1666)
                     /locus_tag="PLU11_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59331.1"
                     /translation="MKYFKLTDLITQTTLMLTSLVLLCFDQAKENAFVLFYFILGGWQ
                     FLSFTIHQFATSESWYHRKHRLFYGKTIGLTFAGFIGLLLLTFFQVPFLFYYLWAALF
                     ITPLYALGYLIISYRELQTIQRKELIHLKN"
     gene            complement(1757..2263)
                     /locus_tag="PLU11_09170"
     CDS             complement(1757..2263)
                     /locus_tag="PLU11_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPN59332.1"
                     /translation="MKLTEAKQQFISSWGAFGTHWGINRTMAQIHALLLVSPEPLTQD
                     DIMEELNISRGNTNMNIRELINWGLVERVLISGERKEFFSAEKDIWKVVKQIVKERKK
                     RELEPMLQLLDKLEAVEGDKKDKEVKTFVDTISGIKKLGKQADKTLDTMIRAEESWFV
                     GALMKVFK"
     gene            2355..4202
                     /locus_tag="PLU11_09175"
     CDS             2355..4202
                     /locus_tag="PLU11_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPN59333.1"
                     /translation="MKQLLVLFLFIISFSGYAQQRVFRFAFISDTHIGSPNGSAEEDL
                     RRSIRDINAQEDIAFVVLTGDITEMGTNDELKLARQILDSLGLKWYIIPGNHDTGWSE
                     SGGDMFKTVFGSDKFSFEYNGIRFIGCASGPYVRMSDGHVPRDAVNWLDQELRQLKPG
                     QPLVFLNHYPIDEGLDNWYEITDRLRQYNTQVILCGHGHANKAMNFEDIPGVMGRSNL
                     RAKATEGGYNLVEWRNDSLIFTERKPVTGKEKHWTSVALSTHHYQKGKTFKRPEYTVT
                     KKESRVTVSWSVSDEANIISTPALAKDRVIVGNQLGRINGYDYSNGKKKWAFHADGAV
                     FSSPAVAGNRVVLGSADSTIYCLDARNGKLQWTYKTNAAVLGAPLTNGDTVFIGGSDH
                     HYRAIRVSDGKLIWDFDGLQGPVVSKPLLVAGKLVFGAWDRHLYAVSSATGQLLWKWN
                     NGSSVRNYSPASCIPVAADGVVYIMAPDRFITAIDLTSGNTLWRNNDGGVRESIGISA
                     DGKWIYGKSMQDSIVAYPASREKQSAAWKMHAGFGYEHAPSMLIEKEGQVFFGTRSGQ
                     VFSIDPKNKRVNWRYKIDNSMVNTVQVVDATHLLVSTMDGKLVLLESGK"
     gene            complement(4206..4430)
                     /locus_tag="PLU11_09180"
     CDS             complement(4206..4430)
                     /locus_tag="PLU11_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59334.1"
                     /translation="MAEKDESGFSWLSLFLVTGFSGLGFDLIIKLLDGYHSPILLWIG
                     IIALSLGLLNFLISLIARKASNKKESRLGD"
     gene            complement(4677..>6337)
                     /locus_tag="PLU11_09185"
     CDS             complement(4677..>6337)
                     /locus_tag="PLU11_09185"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HPN59335.1"
                     /translation="YMAEVILMPRLSDTMTEGVIAAWHKKVGDPVKKGDLLAEVETDK
                     ATMELESYKDGVLLHIGTEKGGKLQVNDLLAIIGTAGEDISGLMGAASAAPATNAPVA
                     APEAPAATAPSATAASSLDISKMDEVVLMPRLSDTMEAGVIASWNKKVGDTVKKGEVL
                     AEIETDKATMELESYKNGTLLYVGAEKGEKILVNGLLCVIGEKGKVDVDAIIAASKAG
                     VTTATAPPPASTPAVAAPAPVAATAVQTAPVTTTTAITENGRVKASPLAKKIAQEKGV
                     DLRLLQGSGDGGRIVKADIDNYKPGTAPAAAAAVAPVAAATVAVFAGSTEEGYTDLPN
                     SQIRNVIARRLGESKFSAPHFYLTMEINMDNAMAARTQLNEISPAKISFNDLVVKAVG
                     LALRQHPAINASWMGDKIRRYKHIHIGIAVAIEDGLIVPVIRFADQKTLPQIAAESKE
                     LAGKAKNKKLQPNEFAGNTFTISNLGMMDIDEFTAIINPPDSAIMAVGRIKETVVRKG
                     DGFGVSNFMKVTLSCDHRSVDGASGAAFLQTFKKYLENPITMLL"
BASE COUNT         1649 a         1459 c         1498 g         1731 t
ORIGIN      
        1 taactgctta acgccggtac ctgggcgcgg gcagtcccgg cacccagcaa cagggccaga
       61 acgggtaaaa aatagttttt cataacaagg cggatttagg attggatagg atacaatggg
      121 tacggactaa aaacgggtca gctataacat caactttttt tattcattga ctaattcata
      181 atagttcttc tttaccagca ccagctggcc atcctggttt tcgagttcat acaggtcacc
      241 atgtttgaaa ctgatgatcc gtcccgaata gcggatactc ccatccggat tcgtgatttt
      301 tgagatagtc aggcgggctc cgttgaaatc atccgagcgg ataaccacac tctgaatact
      361 gttattgtat ttgctggcgg tggaaaccac ttcaccggca aactgcagcg tattgtcctc
      421 tgacaaacga aactgactgt tacgtccgac cggggcactc aggatattgt caatattagt
      481 aagggtaaca ggaatcttgt ccggcaggtt ggagaataaa cggggcttgt tctggtccgg
      541 ttcattcagc ggaatttttt gttcctggct attggaaaca atggcggata gtgtaagtac
      601 tacgcatagc atagtggcac ggaaggtttt catcggttct tcttttaaga gtcaatagga
      661 tattgttcgg caatcaggat tggtgattgc agctttcagg ggggtacggt atgtaaaggt
      721 tggtgaaact tttattcagt tccaaaaaca agcctactga tttctggaaa aatgaggagt
      781 agtacctgcg gcgattcgta gattttcgaa aacgaacctg tttatctacg atttaatact
      841 ggtttgtact taacatgaca agggtgcagg cttcaatggt ctgaatgttt ttttcagcag
      901 ctagcgctgc cagttcatcg ttttcagtgc ccggattaaa aatgatccgc tttggggaga
      961 gagacaggat ataatcataa tattcccgtt gatgctgtgg gttgagatac aacgtcaccg
     1021 tatcaatatc agcatattta tgcttagctg tttctactgt tacatcggct actacggtag
     1081 ccttgcgtcc gatggccagc accggatgac catggctgcg taaacgttgt accgccaggt
     1141 aactgtaccg tgagggattg tcagaagcac cgagcactaa tgttttcttt tgtgacatag
     1201 ggtaaagcta caaagagtag tttaaaccgg ggcgaagttt cctttcgctg tctgaaggag
     1261 tcgccggtca gttttttaaa tggatgagtt ctttgcgctg gatggtctgt aattcacggt
     1321 aactgatgat caggtagcca agcgcataca aaggagtgat aaacaaggct gcccacaaat
     1381 agtagaacag gaacggtacc tggaagaaag ttaaaagcag taatccgata aaaccggcaa
     1441 atgtcaggcc gattgttttc ccatagaaca gccggtgctt ccggtgatac cagctttcgc
     1501 ttgtggcaaa ctggtgtatg gtaaaactga gaaattgcca gccacccagt ataaaataaa
     1561 acaacacaaa cgcattttcc ttcgcctggt caaagcaaag caacaccaga ctggtcagca
     1621 tcagggtggt ctgggtgatc aggtccgtga gtttaaaata tttcatattt aatctatttg
     1681 taatttcaga aaataatgaa aatattggat gaaagaaaag ccggacaaac cggcttttcc
     1741 aaacaaaaca gatacactac ttaaatactt tcatcagggc acctacaaac cagctctctt
     1801 ctgcccggat catcgtgtcc agggttttgt cggcctgctt gccaagtttt ttaatgccac
     1861 tgatggtatc tacaaaggtc ttcacctctt tatctttttt atcgccttcc actgcttcga
     1921 gtttatcgag caactgcagc ataggttcca gttctctttt cttccgttcc ttcacaatct
     1981 gcttcaccac tttccagatg tctttctccg ccgaaaaaaa ttccttccgt tcgccggata
     2041 tcaagacccg ttccaccagt ccccaattga tcagttcccg gatattcata ttggtattac
     2101 cccggctgat attgagttcc tccataatat catcctgggt gaggggttcc ggactcacca
     2161 gtaataaggc atgaatctgc gccatggtac ggttgattcc ccaatgagta ccaaaggctc
     2221 cccagctgct gatgaattgt tgtttggctt ccgtaagttt catatagcaa aattacaggc
     2281 tgttttcgaa atttcaaaaa ttattgaaaa tatatttttg attgtcccgg atatggaatt
     2341 tggtaaattg gctgatgaag cagctcctgg tattattctt atttattatt tctttttccg
     2401 gctatgccca gcaacgggta ttccggtttg cctttatatc cgatacccat atcggttcac
     2461 ccaacggatc cgcagaggaa gaccttcgcc gcagcatccg cgatatcaat gcgcaggaag
     2521 atattgcttt tgtggtgctg acaggtgata tcaccgaaat gggaaccaat gatgaactga
     2581 aactggcccg tcagattctg gacagtcttg gactgaaatg gtatatcatc ccaggcaatc
     2641 atgatacggg ctggagtgaa agcgggggcg atatgtttaa aacggttttc ggatctgata
     2701 aattcagttt tgaatacaat ggcatccggt ttatcggatg cgcatcaggg ccctatgttc
     2761 ggatgagtga cgggcatgta ccacgcgacg ccgtgaactg gctggaccag gaactccggc
     2821 aactgaaacc cggacagcca ctggtattcc tgaaccatta ccccattgat gaaggactgg
     2881 acaactggta cgaaatcact gaccggttac gtcagtacaa tacccaggtt attttatgcg
     2941 gccacgggca tgccaataaa gccatgaact ttgaagatat acctggagtg atgggacgct
     3001 ccaatctgcg ggccaaagcc actgaaggtg gttataacct ggtggaatgg cgcaacgatt
     3061 cactcatttt tacggaaaga aaacctgtaa ccggaaaaga aaagcactgg acttctgtgg
     3121 cactgagtac gcatcattat caaaagggca aaacttttaa acgtcctgaa tataccgtta
     3181 caaaaaaaga atcacgggtt acggttagct ggtctgtttc agatgaagcc aatattattt
     3241 ccacaccggc tttggcaaaa gaccgggtca ttgtgggtaa tcagctgggc cgcatcaacg
     3301 gatatgatta ttccaacggg aaaaagaaat gggcattcca tgctgacggg gctgtttttt
     3361 cctcaccggc cgttgcgggt aaccgggtag tcctgggctc ggcggatagt accatctatt
     3421 gtctggatgc ccggaatgga aaactgcaat ggacctataa aacaaatgcg gccgtgctgg
     3481 gtgcacccct gaccaacggg gatactgttt ttatcggggg gagcgatcat cattaccgcg
     3541 ccatccgtgt ttcagatggc aagctgatct gggattttga tggcttgcag ggaccggtgg
     3601 tcagtaaacc attattagta gcgggtaaac tggtattcgg tgcatgggac cggcatcttt
     3661 atgcggtatc cagtgcaacc ggacaactgc tctggaaatg gaataacgga tcatcagtgc
     3721 ggaattattc tcccgcctct tgtataccgg ttgcggcaga cggcgtggtt tatatcatgg
     3781 cacctgatcg ctttattacc gccattgacc tgactagcgg taacaccctc tggcgaaaca
     3841 atgatggcgg ggttcgggaa tcgatcggca tatccgctga tggtaaatgg atatatggaa
     3901 aaagcatgca ggattccatt gttgcctatc cggccagccg ggaaaaacaa tccgccgcct
     3961 ggaaaatgca cgccggattt ggatatgaac atgcgccatc catgctgatt gaaaaagaag
     4021 gccaggtgtt ttttgggaca agaagcggac aggtgttttc cattgatcca aaaaacaaac
     4081 gggttaactg gcgctataag attgataatt caatggtgaa caccgtgcag gtggtggatg
     4141 ccacccatct gctggtcagt acgatggatg ggaaactggt attattggag tcggggaaat
     4201 aaaggctaat cacccaatct tgattctttt ttgtttgatg ctttgcgggc aatcaagcta
     4261 atcaggaagt tgagcaggcc gagtgaaaga gcaataattc caatccaaag caaaatgggt
     4321 gaatgataac catccagcag tttgatgatc agatcaaacc ccagtcctga aaagccggtt
     4381 accaaaaaca ggctcagcca ggaaaaacca gactcatcct tttcagccat agtatgattt
     4441 taaggaaagg taaaatggtt ttatttataa aatcatgagt tacaatgcgg gattttctca
     4501 tttaaggcat tccccctatt gactctaagt gactctattc aagggcaatt atactgccat
     4561 ttttccggaa aaggatgagt ttgcaaaaat cgggaagtgc aataacattc aagggcataa
     4621 aaaagggtaa gcctgaatga cttaccctga taaataatat tcagctcttt tcctactcag
     4681 agcaacatcg taatcggatt ctccaggtac tttttaaatg tctggaggaa ggcagcccct
     4741 gaagctccat ccacactgcg gtgatcgcaa ctgagcgtta ctttcataaa gttgcttaca
     4801 ccaaaaccat cgcctttcct taccaccgtt tccttgattc ggccaacggc catgatggca
     4861 ctgtccggtg gattgataat cgctgtaaat tcatcaatat ccatcatacc caggttggaa
     4921 atggtaaatg tatttcctgc gaattcgttg ggttgtaatt ttttattctt ggctttaccg
     4981 gcaagttctt tgctttcagc ggcgatttgt ggtaaagttt tctgatcggc aaaacgaatc
     5041 accggcacaa tcagtccatc ctcaatagcc acggctatac caatatgaat atgcttgtac
     5101 cggcggattt tatcacccat ccaggaagca ttgatcgccg gatgctggcg aagggccagt
     5161 ccgacagctt ttaccaccaa atcattgaaa ctgatcttgg caggactgat ctcgttgagt
     5221 tgtgtacgcg ccgccatcgc attgtccatg ttaatttcca tcgtcagata gaaatgcggc
     5281 gcactgaatt tactttcacc caaccggcgg gcaatcacat tgcggatctg ggaattgggc
     5341 agatccgtgt atccttcttc ggtgctgccg gcaaatactg ccacagttgc cgctgccaca
     5401 ggggcaacag ctgctgccgc agcgggggcc gttcctggct tatagttatc aatatccgct
     5461 tttacaatac gtccgccatc tccgcttccc tggagtaaac gaagatccac gcctttttcc
     5521 tgggcaattt tcttggccag gggagaagct ttgacgcgtc cattttcagt tattgccgtt
     5581 gttgtggtta ccggcgctgt ttgaacagcc gttgcagcca caggagcagg agccgcaact
     5641 gcaggggttg atgcaggagg aggagccgtt gcggttgtaa ccccagcttt actggccgca
     5701 ataatggcgt ctacatccac cttccctttt tctccaataa cacataatag cccgttcacc
     5761 agaatttttt cgcctttctc tgcgccgaca tataacaggg ttccgttttt ataactctcc
     5821 agttccatgg tggctttatc tgtttctatt tcagccagta cttcgccttt ctttaccgta
     5881 tcgcctactt ttttattcca ggaagcaata acgcctgctt ccatcgtatc actcagacgg
     5941 ggcattaaaa ccacttcatc cattttggaa atatcaagcg aagacgcggc tgtagccgaa
     6001 ggtgcagttg ccgccggagc ttcgggcgcc gcaacagggg cattggtggc aggagcagct
     6061 gaggcggctc ccatcagacc agaaatatct tccccggctg taccaataat ggccagcaag
     6121 tcatttacct ggagttttcc gcccttttct gtgccgatat gcaggagtac cccatcttta
     6181 tagctctcca gttccattgt ggccttgtcc gtttccactt cggccagcag atcccctttc
     6241 tttacaggat ctcccacttt tttatgccag gcggcgatca caccttctgt cattgtatcg
     6301 ctcaggcggg gcattaatat cacttcagcc atgtacc
//
LOCUS       DAOPMW010000176         6307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14211_length_6307_cov_8.717530, whole genome shotgun sequence.
ACCESSION   DAOPMW010000176 DAOPMW010000000
VERSION     DAOPMW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14211_length_6307_cov_8.717530"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLU11_09190"
     CDS             complement(<1..891)
                     /locus_tag="PLU11_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59336.1"
                     /translation="MKQVTICLAMAVMVMAGNHIYAQGCVAIRGVAGVCGRPADANGW
                     DMNINNRYFKSYKHFVGTEEQKERVENGSEVINHSYELDITATRTFNTRWSVGLTLPI
                     MAFGRSSLYEHDRQNRYSTHSFGIGDIRVAAYRWMFDPAKSHKGNLQLGLGIKLPTGV
                     YNYQDYFYKKPDSAVLGPVDQSIQPGDGGTGISVELNSYYNFNHKVGAYGSFFYLINP
                     REVNGTSTSRGSAPSATAIKYNTDVMSVPDLFMARGGLSYMLGHVTFSGGIRAEGLPS
                     SDLIGGSRGFRRPGYIISAEP"
     gene            complement(919..2244)
                     /locus_tag="PLU11_09195"
     CDS             complement(919..2244)
                     /locus_tag="PLU11_09195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HPN59337.1"
                     /translation="MRIALLAIAAWLAVSACNTKNDYKEITSNPFLYSRTVKELNNVV
                     MDNNFPPIIASRNYLYANIAAYEIIAAGYPDQYQSLAGQVNGFTAVPAAPAGQEIDFH
                     FASILAFCKLGEAVTFPEGSMQFYVDSLKKLVKEHGMPGSVFKNTVAYADTVGKAILA
                     WSKKDNYAQTRTAPKYTVIDTPGRWIPTPPMYAQALEPHWNQIRTMVMDSADMFTPPP
                     PPPFDIKNKNSLYYKEVMAVKNAGDSLTDEQMHIAEFWDDLGTRLNVSGHVMFMTKKF
                     SPTGHWMNIVGIAAQTAKVDFNTTVYAYAKTAIAMFDAFIQCWDEKFRSNTVRPETVI
                     NKYIDADWKPFLQTPPFPEYTCGHSTVSSSAAEALTSVFGDNFVYTDTSELEFGIKNR
                     SFTSFRQAALENNWARFYGGIHFHNSCIVSTEYGRKVGDLINQRLRMKK"
     gene            complement(2302..2856)
                     /locus_tag="PLU11_09200"
     CDS             complement(2302..2856)
                     /locus_tag="PLU11_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59338.1"
                     /translation="MKKIIAICFAGILLFSTGGYHPFISLLQQKADRNLESLLDEEVY
                     DESRLIEIRVPLNMPYQQRYTGYERHYGNIEVEGQSYTYVKKKIDGDVVIFQCIPNVS
                     RQQLKDMRYDLTRANSNIATTEQGPPQQKQPSLIKNSLGDFDDQLNTAFQKKPANPAN
                     SFNDNYLLYPVEMNGKVPHAPPEC"
     assembly_gap    2989..3088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3089..5066)
                     /locus_tag="PLU11_09205"
     CDS             complement(<3089..5066)
                     /locus_tag="PLU11_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HPN59339.1"
                     /translation="MRRLLCLTLYISITGLSVTGQPDPWKIKSGKPDPGNYYGITVAN
                     GMIGIVSSPEPFKVKEVVLAGAYDLYGRGRVSNFLKSFNLLNMRLDIDGQSIGSNNAL
                     NLQQELDMKKAAFTTSFDYGDKARISYTYYSLRQLPYTVLMDISITAKKDFRLGGASV
                     MEAPDALKEVQNYYNEIDRPHVTISLLTSTAKSPTGKLQLCASNTFLFPEAHGQEPRV
                     IHEMWDNNMHLMKFSRNVKAGETYRFSIVGSSITSAHHDDPLNEAERASIFAKLEGRE
                     RLLQFHTQAWDELWKSDITIEGDPQAQQDIHSMLYHLYSFTREGTALSPSPMGLSGLG
                     YNGHVFWDTELWMYPAMLVLHPELAKSMIEYRYQRLEAAKRNAFSKGYKGAMFPWESA
                     DSGVEETPVWALSGPFEHHITGCVAIAAWNYYCVTQDKNWLREKGWPLLSATADFWAS
                     RVERKGPGQYDINNVVAADEWAENVNNNAFTNAAARANLQFATEAANLLGIAPDPVWM
                     QVHANIPILKFPDGVTKEHAAYAGEGIKQADVNLLSYPLKEVTDPKAIRKDLAYYETR
                     VPNEGTPAMTQAVFTTLYARLGEGEKAYHWFKDAYLPNLLPPFRVIAETKGGTNPYFA
                     TGAGGILQSILMGFGGLDITPQGLVQLKSALPGHW"
     gene            5453..>6307
                     /locus_tag="PLU11_09210"
     CDS             5453..>6307
                     /locus_tag="PLU11_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN59340.1"
                     /translation="MLPQKLMKAASLIILALLAQISFAQDRVITGKITDSKDGSPVQG
                     ASVVAKGNRTGAQTGVDGTFRISVPATVNTLIVSSVGFATQEVSIEGKTSVEVSLVVV
                     NASMGEVVVVAYGTRRRGDLTGSVTSVSAKDFQKGNINSPEQLLQGKVAGLQVTTGGG
                     SAGGGSRIRIRGGASLNASNDPLIVIDGVPVEGNGVAGSANLLNSINPNDIESMTVLK
                     DASATALYGSRASNGVLIITTKKGMKGKMKFTYNTMFSVGIIGDKVDVLSGDQVRSII
                     DADAAATGN"
BASE COUNT         1551 a         1461 c         1492 g         1703 t
ORIGIN      
        1 aggttctgct gaaataatat aacccggtct cctgaatccg cgacttccac cgatcaggtc
       61 actggaagga agtccttccg cacggatacc acccgaaaaa gtaacatgcc ccagcatata
      121 actcagtccg ccacgggcca taaacagatc gggcacactc atgacatcgg tattgtattt
      181 aatggccgtg gccgatggag cactgcctct tgaagtggaa gtgccgttca cttcgcgggg
      241 attgatcagg taaaagaaac tgccataagc gcctacctta tgattgaagt tgtaataact
      301 attcagttca acactgatgc ctgtaccccc atcgccgggt tggatggatt gatccaccgg
      361 gcctaataca gctgaatcgg gtttcttgta aaaataatcc tggtagttat atacccccgt
      421 tggcagtttg atacccagtc ccagttgcag atttccttta tgtgattttg caggatcaaa
      481 catccagcgg taagcagcta cccggatatc gccgatacca aatgagtggg tactgtaacg
      541 gttctgccgg tcatgttcat acaacgaaga acgaccgaat gccatgatgg gcagggtcaa
      601 gccgaccgac caacgggtat tgaatgtacg ggtagcggta atatccagtt cataagaatg
      661 attgatcacc tcgctgccgt tttccacccg ttctttttgt tcttccgtgc ctacaaaatg
      721 tttataggat ttaaaatacc ggttgttgat attcatatcc catccgttgg catcagcagg
      781 gcggccgcac acaccggcta caccccggat ggcgacacaa ccttgtgcat atatatggtt
      841 gccggccatg accataacgg ccattgccag gcaaatagtt acctgtttca tgtaaaatcg
      901 ttggtttttg tttcgcaatt attttttcat ccggagacgc tggttgatca ggtcccccac
      961 ttttcttcca tactctgtac tcacgataca ggagttatga aagtgaatcc ctccgtaaaa
     1021 acgggcccag ttattttcaa gggccgcctg gcggaaagaa gtaaatgaac ggtttttaat
     1081 gccaaactcc agctccgagg tatcggtata aacaaaattg tcgccaaata cactggtcag
     1141 tgcttcagcg gcagaagaag agacggtgct atgcccgcag gtatactccg ggaagggagg
     1201 agtctgcagg aagggcttcc agtccgcatc aatgtattta ttgatcacgg tttccggacg
     1261 aacggtatta ctcctgaatt tctcatccca gcactggata aaggcatcga acatggcaat
     1321 ggccgttttc gcataggcgt atacggtagt gttaaaatcc actttcgccg tctgtgcggc
     1381 tataccgaca atattcatcc agtgacccgt gggggagaat tttttggtca taaacattac
     1441 gtgaccggaa acattcagcc gggtacccag gtcgtcccag aattctgcga tatgcatctg
     1501 ctcgtctgtt aaactgtcgc ctgcgttttt gaccgccatt acttctttgt aataaagact
     1561 gtttttgttt ttaatatcaa atggcggggg cggaggcggt gtaaacatat cggcactgtc
     1621 catcaccatg gtccggatct ggttccagtg tggctccagt gcctgtgcat acatcggagg
     1681 tgttgggatc cagcggccgg gggtatcgat cacggtatat ttgggagccg tcctcgtttg
     1741 cgcatagtta tctttcttac tccaggccag gatcgctttt cctactgtat ctgcataggc
     1801 aaccgtgttt ttaaaaacgg aacccggcat accatgctcc ttcacgagtt ttttcaggct
     1861 gtccacataa aactgcatac tgccttcggg gaacgtaacg gcttcaccaa gtttgcagaa
     1921 tgccagtata ctggcaaaat gaaaatcaat ttcctgtccg gccggtgctg cgggaacagc
     1981 tgtaaagcca ttcacctgtc cggcgagtga ttggtactgg tcagggtatc cggcggcaat
     2041 gatctcataa gccgcaatat tggcatagag ataattccgg gaagcaatga taggcgggaa
     2101 attattatcc atcaccacat tgttcagctc tttcaccgtt ctggagtaga ggaaaggatt
     2161 actggtgatt tctttataat catttttagt attacaggca cttacagcta accatgctgc
     2221 aatagccaga agggctatac gcatacgaat tcgattgttg tttgaatgaa agttatttaa
     2281 atgaaccgga gtttgttaga atcagcactc cggaggcgca tggggaactt taccattcat
     2341 ctcaaccggg taaagcaggt agttgtcatt aaatgagttt gccggattcg ccggcttttt
     2401 ctgaaaagcg gtattcagct gatcatcaaa atccccgaga ctgtttttga tcagggatgg
     2461 ttgtttttgt tggggcggac cttgttccgt agtcgctata ttactgttgg cgcgggtaag
     2521 gtcataccgc atatctttca gttgctggcg ggaaacgttg ggaatacact ggaagatcac
     2581 tacatcgccg tcgatctttt tctttacata ggtgtatgac tgcccctcca cttcaatatt
     2641 tccgtaatgt cgctcgtaac cggtatagcg ttgctggtat ggcatattca acggcacgcg
     2701 gatctcaatc aggcgggact catcgtatac ttcttcgtca agcagggatt ccaggttgcg
     2761 gtcagccttc tgctgcagca ggctgataaa agggtggtac cctcctgtac tgaacagaag
     2821 aattccggca aagcatatgg caattatctt tttcaaagca gcagtcgact gattctattg
     2881 acgcacaata tatgtttttt tctctcttcc cgcaccggtg atggtcaggc ttttccaatg
     2941 accgggtaag gcagatttta gctgcaccag tccctgcggg gtaatatcnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc caatgaccgg gtaaggcaga ttttagctgc
     3121 accagtccct gcggggtaat atctaatccg ccaaaaccca tgagtatact ttgcaaaatc
     3181 cctccggccc ccgtggcaaa atagggattg gtaccaccct tggtttcggc gatcacccgg
     3241 aagggaggca acaggttggg cagataagca tccttaaacc agtgataggc tttttcacct
     3301 tcccccagcc gtgcatataa ggtagtgaat accgcctgtg tcatagcggg tgtaccctcg
     3361 ttgggtaccc gtgtttcata ataagcgagg tctttacgga tggctttcgg atcagtcact
     3421 tcttttaatg gataagacaa cagatttaca tcggcctgtt tgattccctc accggcatag
     3481 gccgcatgtt cttttgttac gccatccggg aatttcagga tgggtatatt ggcatgcacc
     3541 tgcatccaga ccggatcggg ggcaatgcct aaaaggttcg ctgcttcggt agcaaactgc
     3601 agattggccc gggcagctgc attggtaaag gcgttattat ttacattctc cgcccactca
     3661 tcggcggcta ctacattatt aatatcatat tgaccaggac cttttctttc cacgcggctg
     3721 gcccagaaat cagcggtggc tgataataac ggccaacctt tttcacgcaa ccagttttta
     3781 tcctgggtta cacaataata attccaggca gcaatggcca cacagcccgt gatatgatgt
     3841 tcaaaggggc cgctcaaggc ccataccggt gtttcttcca caccggaatc cgcactttcc
     3901 cagggaaaca tggcgccctt ataacctttg ctgaatgcgt ttctcttcgc agcctctaac
     3961 cgctggtaac ggtattcgat catactcttt gccagttccg gatgcaatac gagcatggcg
     4021 ggatacatcc acaattccgt atcccagaac acatggccat tgtagcccag tccgctcagt
     4081 cccataggcg atggcgacaa agccgtgcct tcccgcgtaa acgaatacaa gtgatacaac
     4141 atactgtgta tatcctgctg ggcctgtgga tcgccttcaa tggtaatatc acttttccag
     4201 agttcatccc aggcctgggt atgaaattgc agcagccgct cccttccttc cagtttcgca
     4261 aagatgctgg ccctttcggc ttcattcagc ggatcatcat gatgggcaga agtaatggaa
     4321 gagccaacaa ttgagaaacg ataggtttcg ccggctttca cattccggga aaatttcata
     4381 aggtgcatat tattgtccca catttcatga atgacgcggg gttcctgtcc atgtgcttcc
     4441 gggaataaaa atgtattact cgcacagagc tgcagtttac cggtcggact cttcgcggtg
     4501 gaggtaagca gactgatcgt tacatgggga cggtcaattt cattgtaata attctgtact
     4561 tcttttaagg catccggcgc ttccatcaca ctggcaccgc ccaggcgaaa gtcttttttt
     4621 gccgtgatgg aaatatccat caatacggta tagggcagct gtcttaatga gtaataggta
     4681 taactgatcc gggctttgtc tccataatca aaggaagtgg taaacgccgc ttttttcata
     4741 tccagttcct gttgcaggtt gagggcatta ttactgccta tactttggcc atcaatatcg
     4801 agccgcatgt tgagcaggtt aaagcttttc agaaaattgc tcacccggcc ccggccatag
     4861 agatcatagg cgccggccag cactacttct tttactttaa aaggttcggg ggaggaaaca
     4921 atcccgatca ttccgttggc aacggtgatc ccgtaataat ttcccgggtc aggtttaccg
     4981 gatttgatct tccatggatc cggttgacca gtaacagaga gcccggttat acttatatat
     5041 aatgttagac aaagcaatcg tcgcatacag tcaatttgaa gtaaattaag cagtccttgc
     5101 ttaccatccg gtgttatgga tttacgaaaa cgattccggt ggataatcca tctgcccttt
     5161 ttttgccgta gctgtaaagc gtttttgggt gattctaatc agccagaccg gctgacttaa
     5221 gcccttcctg ggaccggaac atattgttta aatattgtta atcaatgatt tgatttatta
     5281 aacaaggtat tctgcactgg aatttttttt gcagattcag attctttttt aaaaataatt
     5341 tttggaagcc ttaaaataat gctaatattg tgtgtttaat acacattcaa atgtgtcttt
     5401 gatacataat gtgtggatga taacaagatt gctaaaaatt taatgattgc ttatgttgcc
     5461 ccaaaaactg atgaaggcag ccagtcttat aatactggcg ttattggcgc agatctcctt
     5521 tgcgcaagac agagtgatta caggaaaaat tactgattcc aaagatggtt cccccgtaca
     5581 gggagcaagt gtggtggcca aaggtaaccg caccggtgct cagactggcg ttgacgggac
     5641 tttccgcatt tcagtacctg ctactgtaaa cacactgatc gtatcttctg ttggctttgc
     5701 cacacaggaa gtatctattg aaggaaaaac ttcggtggaa gtatccctgg ttgtcgtcaa
     5761 tgcttcgatg ggcgaagtag tggtggtagc ctatggtacc cgcagaaggg gagacctgac
     5821 cggatccgtt acatcggtat ccgccaagga tttccagaaa ggaaatatca actcacccga
     5881 gcaattgctt cagggtaagg tagctggttt gcaggttacc accggtggtg gttctgccgg
     5941 cggcggcagc cgtatccgga tccggggtgg tgcttccctg aatgccagta atgatccgct
     6001 gatcgttatt gacggggtgc ctgtagaggg taacggggtg gctggttccg ccaacctgct
     6061 gaactccatc aacccgaatg atattgaatc catgacggtg ctgaaagacg cttctgcaac
     6121 cgcgctgtat ggttcaagag cctctaacgg ggtgctgatc attaccacta aaaagggaat
     6181 gaaaggaaaa atgaaattca cgtataatac catgttctcc gtaggtatta tcggcgataa
     6241 agtggatgta ctgagcggtg accaggtacg cagtatcatt gatgccgatg cggctgctac
     6301 cggaaat
//
LOCUS       DAOPMW010000177         6294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14252_length_6294_cov_7.675108, whole genome shotgun sequence.
ACCESSION   DAOPMW010000177 DAOPMW010000000
VERSION     DAOPMW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_6294_cov_7.675108"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PLU11_09215"
     CDS             <1..1236
                     /locus_tag="PLU11_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN59341.1"
                     /translation="TIPVLDKPNLSLGFRDTLICRNDAVQLNASTTGSGIFSWTPVTN
                     IINANTGTPTVTPPVTTLYFVNLNENGCVNRDSVNVRVIDAVSVTAIPDTTICRGDTI
                     QLSAVTNGLSYSWDPPLNVDNPTLINPRAMVNANTVFTVTSTVGGCSATDQVIVTTVP
                     YPVANAGPDPTICYNASVQLNASITGSSFNWSPPNYLDNPLILNPVASPPGTTQYILS
                     VFDTQGCPKPGKDTILVTILPKVVAFAGRDTSVIVGQPLQFNGTGGTGYFWTPGTSLS
                     STSIPDPIGNYDGSFDNIRYKLIVTDQAGCADSAYITVRIFKTVPSVFVPTGFTPNND
                     GLNDVVKPIAVGMKQINYFSIYNRWGQLVFTTRINGQGWDGRIKGELQGTGVYVWMVS
                     AEDYTGKAYFQKGTVTLIR"
     gene            1297..2064
                     /locus_tag="PLU11_09220"
     CDS             1297..2064
                     /locus_tag="PLU11_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN59342.1"
                     /translation="MGKIVLITGATSGFGKAAAYKFAANGYDLIINGRRTERLDQLAA
                     ALESKYNVAVLALPFDVRDEQAVFNSFTSLPEDWKAIDVLVNNAGLALGRDYFEEASL
                     EDWNTMIDTNIKGVMYVARAVSLLMIPRKKGHIINLGSIAGKEVYEKGNMYCATKAAV
                     DALSEAMRIDLLRHCIKVTAIHPGAAETEFSLVRFKGDPVQAKKIYEGLTPLTGDDVA
                     DVIFYCASLPPHVCINDLTLTPLRQANSFYYNRDTNA"
     gene            2266..2772
                     /locus_tag="PLU11_09225"
     CDS             2266..2772
                     /locus_tag="PLU11_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59343.1"
                     /translation="MKKPYLLLISLLTLSLTSTAQDVIRVQSCSNDMISKQADSLKNL
                     YGNDGYILLKEASINMESEFEMPVIVPLTQGSWYQFVFIGDYSSRLYEVRMYDWNEKQ
                     VVFKQKKWGDVDGNVIVYSYIPQFSEFHLMKPVQVNKQKKKNLCGYVMLFKRNGSPTG
                     TPAPQQPE"
     gene            complement(3100..>6294)
                     /gene="sprA"
                     /locus_tag="PLU11_09230"
     CDS             complement(3100..>6294)
                     /gene="sprA"
                     /locus_tag="PLU11_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPN59344.1"
                     /translation="RIGNDFSGNYYEIKIPLKITQFGETDSALIWPEENYLDFDLDDL
                     TDLKLKRNGLNISPSQYYKETLASGRSYAIVGNPNLGEVRGVLLGVESVNQENVCTEV
                     WFNELRLQQLDETGGTAALARVDMKLADLGTLSVSGTMKTRGFGTLEQRVNERSREDV
                     YTIDASANLEMGKLFPKKLGLQIPVYTSISRRTSTPQYDPYDLDIELKQKLKDADAAK
                     KDSIKNDAQDVTTIKTITLTNVRKVKTDGKRPKIWSVSNFDFNYSYIQTVTHNPLTEN
                     DELRRTRGAIGYSYAPQVKTVEPFRKFIKSRSPWLALVKDFNFNYAPSQISIRADVFR
                     QFGATRPRNVGGGPYQIPETYNKYFTFDRYYILQWNLTRSLSMDFTATNNARIDEPEG
                     RIDTKLKKDSIKTNLFKGGRNTNYGQELTLNYNVPTTKIPLLDWTTLRASYNTKYNWL
                     AASLLARNLGNTLSNTQTRSLNGELKFEELYNKWRFLRAVGSDAPNTGGDNSGGNKGQ
                     KGGKGGKTGDSKADGKNGDGKGNTATGDTKGGDQINNPAQPGVSVSASGVSYRMDTIR
                     NKKGKIVRIKKRKIKVPKAQKPMPEIAAVPKFFLRLATSLKRVGIQYTEDYGTTLPGY
                     MDSTEVFGTNLKSGQPGLKYILGYQPDTNWINSFGAKGLLSRDSLISAMIQQRYNQRM
                     NITAQLSPFRDFTVDVNLDKTFDKQYSELYKDTTGSSGLARLNPYALGSFSVSYISYQ
                     TLFSKFDPNVVSATFKQFEANRRILSGKLASSNPYAAGNTVGADGYVEGYGRYAQDVV
                     IPAFIAAYTNKDPASVKLVKNSNPNIKANPFSGIIPKPNWTLTYTGLSRLPGMDKIFS
                     NVTIRHGYRSTFSMNSFNTALLFTDPLRVGYPSFTDTLTGNYIPYFLVPNITIQESFD
                     PLIQVELTFTNQLTTGFEFKKSRTLSLSLIDYQLAENRSTEVTFNFNWRKKGMPLIKR
                     IGKMKLDNDVTFRLDFSLRDDATANSKLDQGTAFGTGGQKVIRIAPSIDYTLNNRISM
                     KLFFEQNRNIPKISSAFPITNTRGNLQVRVSLAQ"
BASE COUNT         1576 a         1550 c         1424 g         1744 t
ORIGIN      
        1 accataccgg tactggataa acccaacctt agcctcggtt tccgggatac cctgatctgc
       61 cgcaatgatg ctgtccaact caatgccagc actaccggca gcggcatttt tagctggaca
      121 cccgtaacca atatcatcaa tgccaatacc ggcactccta cggtaacacc gccggttacc
      181 actttatatt ttgtaaacct gaatgagaac ggatgtgtca accgcgattc ggtcaatgtt
      241 cgggtgatcg atgccgttag tgtcaccgcc atccctgata ccacgatctg ccggggtgac
      301 acaatccagc tgagcgccgt cactaacgga ctctcctatt catgggatcc gccactaaac
      361 gtcgacaatc caaccctgat caatcccagg gccatggtca atgccaatac cgtattcaca
      421 gtaacatcca cggtaggtgg ctgctcggcc actgatcagg tgattgttac tactgttcct
      481 tacccggtcg ctaatgccgg tccggacccg actatttgct acaatgcttc cgtccagctc
      541 aatgcgtcaa taaccgggag cagctttaac tggtcgcctc ccaattatct tgacaatccc
      601 ttaatcctga atccggtggc gtctccaccc ggaaccacac aatatatcct ttccgtcttc
      661 gacacccagg gctgtcctaa accagggaaa gataccatcc tggtaaccat cttacccaag
      721 gtggtcgctt ttgcaggccg ggatactagc gttattgtgg gacagccgtt gcaattcaac
      781 ggaaccgggg gaaccggtta tttttggacg ccgggcacca gcctgagcag taccagcatc
      841 cccgatccga tcggaaatta tgatggcagc tttgataata tccgttataa actgattgtt
      901 accgaccagg ccggttgtgc cgattctgct tacatcacgg tccgcatttt caaaaccgtt
      961 ccttctgtat ttgtacccac cggatttaca cccaataacg atggactgaa tgatgtggtt
     1021 aaacccattg ccgtaggtat gaaacagatc aattatttca gtatttataa ccggtgggga
     1081 caactggttt tcaccacccg tatcaatggt cagggatggg atggccggat caaaggggaa
     1141 cttcagggca cgggtgttta tgtctggatg gtcagtgccg aagactatac cgggaaagcc
     1201 tatttccaga aaggtaccgt taccctgatc cggtaaaaat ccggatgcgg ctgccaaata
     1261 tggcgtaaac ttgtgtccat tttcaaccga ttcattatgg gaaaaattgt attgatcacc
     1321 ggagccactt cgggttttgg taaagcagct gcttataaat ttgccgccaa tggatatgac
     1381 ctgatcataa atggccgcag aaccgaacgg ctggaccaac tcgccgcagc actggaaagc
     1441 aaatacaatg tggctgtact ggccctgccc tttgacgtgc gggatgaaca agccgttttt
     1501 aattctttta cttccctgcc ggaagactgg aaagccatag atgtcttggt gaacaatgcc
     1561 ggtcttgccc tgggccgtga ttattttgaa gaagcgtccc tggaagactg gaacacgatg
     1621 atcgatacca atatcaaagg tgtgatgtat gtggcgcggg ccgtttcact attgatgatt
     1681 ccccgcaaaa aaggacatat catcaacctg ggatcgattg ccggtaaaga agtgtatgag
     1741 aaaggaaata tgtattgtgc caccaaggct gctgtggatg cactcagtga agccatgcgg
     1801 atcgacctgc tgcggcattg tataaaagta accgccatcc atccgggagc agcggaaacc
     1861 gaattttccc tggttcgttt taaaggtgac ccggtgcagg ctaaaaaaat atatgaaggc
     1921 cttacccccc tgacaggaga cgacgtggcc gatgtgattt tttactgcgc gtccctgcca
     1981 ccccatgtct gcattaatga cctgaccctc actccgctgc gccaggcaaa ttccttttat
     2041 tacaaccggg atacgaacgc ctgatcgtaa acaggagtat gaagtatcgg aatttctttc
     2101 tatattaggt gttcttccgc cctatcacct ccgttgaccc cgtgtatatc cgtatttaac
     2161 atagaaaata tacttaaacc actaagattg cgttcaaaat agtaagcaag cggtaaaaca
     2221 cgtagtaact ttatgtcagc actccaatat ttgataagca ctgttatgaa aaagccgtac
     2281 ctcttactga tttccctgct tacactcagc ctcacttcga cggcacagga tgtgatccgt
     2341 gtacaatcct gcagcaatga catgatcagc aagcaggccg acagccttaa gaatttatat
     2401 ggaaatgacg ggtatatcct tttgaaggaa gcatccatca atatggaaag tgaatttgaa
     2461 atgcctgtga tcgtccccct gacccagggc agctggtacc agtttgtctt tatcggcgat
     2521 tacagctccc ggttgtatga agtacgcatg tacgactgga acgaaaaaca agtggtattt
     2581 aaacagaaaa aatggggcga tgtggatggc aatgtgattg tgtattctta catcccgcag
     2641 ttctctgaat tccacctgat gaaaccggta caggtcaata aacagaagaa gaaaaatctt
     2701 tgcggatatg tgatgctctt caaacgcaac ggatctccca ctggtacacc agcaccccaa
     2761 cagcctgaat aattttagta cgtttcagaa ataattaaac caccgatgag gtggtttttc
     2821 tttttggagt tcacccggaa tttgatataa taattccacc ctttcagggt ttccgaagtg
     2881 aaacgatttc tgtcccgatt tactataatc atttcaccac ttcgtggttt aataaatcgc
     2941 tgaattgttg attcgtgttt atgacattcg acttacgtcc ttcaataaca aaatctccaa
     3001 ggctgtaata aatagaacct tagcaattac tgtggactgt cgactgtgga ctgtggactg
     3061 ccaactgcca actgccgact gccaactgcc gacttctctc tattgcgcca gcgacacccg
     3121 cacctgcaga ttacctctcg tattcgtgat cgggaaggca ctgctgatct ttggaatatt
     3181 ccggttctgt tcgaagaaga gtttcatgct gatccggtta ttcagggtat aatcaatgga
     3241 aggtgcaatg cggatgactt tctgtccacc cgtaccaaag gcagttccct ggtcgagctt
     3301 gctgttggcc gtggcatcat cccgcagact gaaatccagc cggaaagtga catcattatc
     3361 cagcttcatt ttcccaatac gcttgatcag gggcattccc ttcttacgcc agttaaaatt
     3421 aaaggtaact tccgttgacc ggttctccgc cagctgataa tcaatcagac tgagactgag
     3481 ggtacggctc ttcttaaact caaagccggt ggtcagctgg ttggtaaacg tcagttctac
     3541 ctggatgagc ggatcaaagc tctcctgtat cgtgatattg ggcaccagga agtatgggat
     3601 atagttaccg gtcagcgtat cggtaaagct cggataacct acacgcaagg gatccgtaaa
     3661 caagagcgcg gtattgaaac tgttcatgct gaatgtactc cggtatccat gccggatcgt
     3721 tacattgctg aatattttat ccatacccgg taacctcgat aatccggtat aggtaagggt
     3781 ccagttcggt tttggtataa taccagagaa tggattggcc ttgatattcg ggtttgaatt
     3841 cttgaccagt ttcacactgg cagggtcttt atttgtatac gctgcaatga atgccggaat
     3901 caccacatcc tgtgcatagc ggccatagcc ttccacgtat ccgtctgcgc ccactgtatt
     3961 gccggccgca tacggattgc tgctggccag tttacctgat aatatccgcc tgttagcttc
     4021 aaactgtttg aatgtggcag acaccacatt ggggtcaaat ttgctgaaaa gggtctgata
     4081 agaaatataa ctgacactga aactacccag tgcatacgga ttcaaccggg ccagaccgga
     4141 agaaccggtt gtgtctttgt ataactccga atattgttta tcgaatgtct tatccaggtt
     4201 cacatcaaca gtgaaatcgc ggaacggact taactgggcc gttatgttca ttcgctggtt
     4261 atagcgttgc tgaatcatgg cactgatcag tgaatcccgt gataataatc ccttcgcacc
     4321 aaaactattg atccagttcg tatcgggctg ataacccaaa atatatttta atccgggctg
     4381 cccgcttttc aggttcgtgc caaagacttc cgtgctgtcc atataacccg gcaaggttgt
     4441 accatagtct tctgtgtatt gaatacccac ccgcttgagt gaagtagcca accgcaggaa
     4501 gaacttagga acggccgcga tctccggcat gggtttctgt gctttcggca cttttatttt
     4561 gcgctttttg atccgaacaa tctttccttt tttgttccgg attgtatcca tccggtaact
     4621 gacacctgaa gcagaaacgg atacacccgg ttgtgccgga ttgttgatct ggtctccgcc
     4681 ttttgtatct cctgtagccg tatttccttt tccatcacca ttcttaccat ctgcttttga
     4741 atcaccggtc tttccaccct tgccgccttt ctgcccttta tttcctccgg aattatctcc
     4801 gccggtattc ggcgcatcac tgccaactgc ccgcaggaag cgccatttat tatacagttc
     4861 ttcaaatttc aattcgccat tcagggatct tgtctgtgta ttggataaag tatttcccag
     4921 gttccgggca agcagggatg ccgccagcca gttgtacttg gtattataac tggcccgtaa
     4981 agttgtccag tcgagcaatg gaatttttgt tgtaggtaca ttatagttca gggtcagttc
     5041 ctggccatag tttgtattac ggcctccctt gaacaggttg gttttgatcg aatctttttt
     5101 cagcttggta tcaatccggc cttcaggttc atcgatcctt gcattattgg tagcggtgaa
     5161 atccatggac aaggaacggg tcaggttcca ctgcagaata taataccggt caaacgtaaa
     5221 gtatttgttg taggtctcgg gaatctggta aggaccgccg cctacattac gtggacgggt
     5281 ggcaccaaac tgccggaata catcggccct gattgaaatc tgtgatggcg cataattaaa
     5341 attaaaatcc tttaccaatg ccagccaggg cgaccgggat ttgatgaatt tacggaaagg
     5401 ctccaccgtt ttcacctgtg gggcataact atagccgata gctccccggg tacgacgaag
     5461 ttcatcattt tccgtcagcg gattatgcgt taccgtttgt atataggaat aattgaaatc
     5521 gaaattgctg acactccaga ttttcggccg cttaccatcg gtcttcactt tacgtacatt
     5581 ggtcagggta atcgtcttga tggttgttac atcctgtgca tcattcttga tagaatcttt
     5641 ttttgcggca tccgcgtctt tcagtttttg tttgagctcg atatcaagat cataaggatc
     5701 gtactgaggt gtactggtcc ggcggctgat actggtatag acaggaatct gtaatccgag
     5761 ttttttcgga aagagtttac ccatttcaag attggccgat gcatcgattg tatacacatc
     5821 ttcgcggctc cgttcgttca cgcgttgttc cagtgttcca aatccgcggg tcttcatggt
     5881 accggaaacg gaaagggttc ccagatctgc caatttcata tccaccctgg cgagcgctgc
     5941 cgttccgcct gtttcgtcga gttgttgtaa ccggagttca ttaaaccaga cttcggtaca
     6001 aacattctcc tggttcacac tttccacacc cagtaacacc cctctcactt ctcccaggtt
     6061 cggattacca acaatagcat aagagcgtcc gctggcaagg gtttctttat aatattggga
     6121 cggggatata ttcaacccat tccgttttaa tttcaggtct gtcaggtcat caaggtcaaa
     6181 atcgaggtag ttctcttccg gccagatcaa tgcactgtcc gtttcaccaa actgagtgat
     6241 cttcagcgga atcttaattt cataatagtt gccggagaag tcgttaccaa tccg
//
LOCUS       DAOPMW010000178         6195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14549_length_6195_cov_8.206189, whole genome shotgun sequence.
ACCESSION   DAOPMW010000178 DAOPMW010000000
VERSION     DAOPMW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14549_length_6195_cov_8.206189"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PLU11_09235"
     CDS             <1..1723
                     /locus_tag="PLU11_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59345.1"
                     /translation="SKPIPMQFKQSLVRLALILLPISGFSQITFLPQGDKSAMLLDRL
                     EIRAYKDSLFNFSKTRPLSRQHVMNALLSADGKNRLLLNNRVAKSMTKVDQYNLRRIM
                     ANNMEYLPDSLRSAFQAKKPFLKNFYTSPANLYEVHVKDFDLVINPVIQVTVSKEKDN
                     SESLFQNTRGLTLRGRIANKIGFATYLTDNQERVPFYVRDFVTARHAVPGAGFYKSFK
                     GTGYDYFDARGYLTFNVTRYIDVAFGYDRNFIGNGHRSLFLSDFGNSNLFLKLNTRIW
                     KFNYQNLFMELHNADNRLGDRLIGKKYAAMHHLDVAVTKWLNVGLFEGVVFGRPNRFD
                     FGYLNPIIFYRSVEQQNGSFDNSVAGLDVKANLPHKIQVYGQLMLDEFLLSEIKNNRG
                     WWANKWGLQFGAKYIDAFGIPNLDLQLEHNRVRPFTYSHRDSVANYTHYNQPLAHPLG
                     ANFREWIAIARYQPAPKWLITGKLIAYTQGRDSSDRSFGSNIFYPNVAPFRQADFGYE
                     IGSGWKTAVFIGSFLVSYEWKENLFFELSALVRKQETKTAPIVSGNTSVISLGLRWNM
                     HRREFDF"
     gene            complement(1833..2828)
                     /locus_tag="PLU11_09240"
     CDS             complement(1833..2828)
                     /locus_tag="PLU11_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59346.1"
                     /translation="MKKFLLQLFIFLAPLVLLAFVADRYINRTLKTSKTGDYAVWNDI
                     LDGKVNSEIVIYGSSRAWLQMNPRIIEDSTGKDAYNLGIDGHNFYMQYLRHDLLLRYN
                     KPPGTIILSLDNFTFDKKKDLYNPEQFLPYLDNKEILDATKTYFGFDFYDYKLPLIRY
                     AGQQDALKNALKIMIKPSLNNIDRYKGYSGQDLSWNNDLDRAMAVQKKYVQHFDSATI
                     RLFDQFLREARATKTEIIMVYSPEYIGGQQFVSNRKEVFAIYDSFARKYQIPFFDYSK
                     DSMCLDKDFFYNSQHLNSNGANVFTRKMIRDIKAYREQKLQSNGIYKIARPEQGN"
     gene            complement(2831..4270)
                     /locus_tag="PLU11_09245"
     CDS             complement(2831..4270)
                     /locus_tag="PLU11_09245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPN59347.1"
                     /translation="MLFNSISFAIFLPVVFLLYWFATKGKVRLQNILLLVASYFFYAC
                     WDWRFLFLLIFSTLLDYYTGIRIHESATKKKSRYWMWLSIVINLGFLGVFKYYNFFVS
                     SFADGLALAGIKMNYATISVILPVGISFYTFHGISYVLDIYYKRIKPEKNFVDYSVFV
                     SFFPLLVAGPIERATHLLPQIIKERKFEIAKAVDGLRQILWGLFKKIVIADNCAEYAN
                     MIFNNSESYHGGILVLGAVLFAFQIYGDFSGYSDIALGTARLFGIDLLRNFAFPYFSR
                     DIAEFWRRWHISLSSWFRDYLYIPLGGSKGGKWMKVRNTFIIFLVSGFWHGANWTFIV
                     WGFLNALFIMPSVLMNTNRNNLDIVAKGRIFPGIKDLLSILLTFTLTLFAWIFFRADT
                     LSHAFHYIGGIFTTDFFEQSDEFIPRPLYFLLAAFILTEWYGRESRYALEKFGVKYNR
                     IFRWSVYYLIILLLFVFKGNTQQFIYFQF"
     gene            complement(4667..5416)
                     /locus_tag="PLU11_09250"
     CDS             complement(4667..5416)
                     /locus_tag="PLU11_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN59348.1"
                     /translation="MLHILRKKSLIRSLIIPVLLNGLMLVSSSANAQFGKVEMNMFDH
                     DSKPYYFGITIGVNLARFHTEWHPRFLQSDSVLIGEPINSGGLTLGLSATGRISDRFQ
                     VRFNPQLMFIERNIFYKLKYPDFDGNTEVTKKVESVIMSFPIQIKFQSDRIGNFRVYT
                     LAGFKADIDMASNARAKKAEELIKIQKHDFGPEFGIGFNFFFPSFIFSPEIKISNGLR
                     NIHARDENLRFSSMFDKIQSRMIVFSIHLEG"
     gene            complement(5386..6135)
                     /gene="ubiE"
                     /locus_tag="PLU11_09255"
     CDS             complement(5386..6135)
                     /gene="ubiE"
                     /locus_tag="PLU11_09255"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPN59349.1"
                     /translation="MSNPLPHDNIIPFKGSDKTKKEQVADMFDRIAGRYDLMNRFLSA
                     RIDISWRKKAINMFKKDQPQHLLDVATGTADMALMAYSILKPRQITGIDISEGMLEYG
                     RKKIEKEGLGEKIQLHTGDAETIKFADNTFDGVMVAFGVRNFENLEKGLTEIKRVLKP
                     GGQLIVLEFSKPRRKVVKGLYNLYMGIIAPQVARWFRQNKEAYEYLNESANAFPDRHL
                     FTDILKKVGYSETEFQPLTFGICCIYSGRKV"
BASE COUNT         1775 a         1331 c         1385 g         1704 t
ORIGIN      
        1 gtcaaagcca atacccatgc aatttaaaca aagccttgta agactggcgc tgatcctgct
       61 tccaatttcc ggattttctc agatcacttt cctgccgcag ggagataaat cggcgatgct
      121 gctggatcgg ctcgagatcc gggcctataa ggattccctg tttaatttct ctaaaacaag
      181 gccactcagc cggcagcatg tgatgaatgc actgttgtct gcggacggga agaacaggct
      241 attactgaat aaccgggtgg caaagtccat gacgaaagtg gatcagtata atcttcgcag
      301 gatcatggcg aataacatgg agtacctgcc tgacagtctt cgttcagcct tccaggcaaa
      361 aaaacctttt cttaaaaatt tttatacctc cccggccaac ctgtatgaag tacatgtaaa
      421 agatttcgac ctggtgatca atcccgtgat ccaggtaact gtttccaaag aaaaggacaa
      481 tagtgaaagt ctttttcaga atacccgggg gctgacactc cgcggaagga tcgcgaataa
      541 gatcggattt gccacctacc tgaccgataa ccaggaacgg gtgccttttt atgtgcgtga
      601 ctttgtaaca gccaggcatg ccgtgccggg agccggattt tataagagtt ttaaaggtac
      661 gggctatgat tactttgatg cccggggcta cctgactttc aatgtaaccc gttacatcga
      721 tgtggctttt ggctacgacc ggaattttat cggcaacgga caccgcagtt tattcctgag
      781 tgattttggc aacagtaatc tttttctgaa actcaatacc cggatatgga aattcaacta
      841 ccagaacctg tttatggaac tgcacaatgc agataaccgg ttaggggacc gtttaatcgg
      901 caaaaaatat gcagccatgc accacctcga tgtagcggtg accaaatggc tgaatgtggg
      961 attgtttgaa ggagtggtct ttggccggcc caaccgcttt gattttggat acctgaaccc
     1021 gatcattttt taccgttctg tggagcaaca gaatggcagc tttgataatt ccgtggccgg
     1081 actggatgta aaagccaacc tgcctcataa gatccaggta tatggtcaac tgatgctgga
     1141 tgaatttctt ttatcagaga taaaaaacaa ccggggctgg tgggccaaca aatggggatt
     1201 gcagtttggg gcaaaataca ttgatgcctt tggtataccc aacctggacc tgcagctgga
     1261 acataaccgg gtgaggcctt ttacctattc tcatcgtgat tcggtggcca attataccca
     1321 ttacaatcaa cccctcgctc accccctggg ggctaatttc cgcgagtgga ttgccatcgc
     1381 ccgttatcaa cccgctccca agtggctgat caccggtaaa ctgattgcgt atacacaagg
     1441 acgtgatagc agcgaccgga gttttggcag caatatcttt tatcctaatg tggcaccatt
     1501 ccgccaggcc gattttggct atgaaatcgg cagtggttgg aagacggccg tttttatagg
     1561 ctcattcctg gtttcctatg aatggaaaga gaatctgttt tttgaactca gtgccctggt
     1621 ccggaagcag gaaacaaaaa cagctcccat cgtatccgga aatacatcag taatcagtct
     1681 gggacttcgc tggaatatgc accggcggga gtttgatttc tgaccgggta aaagcggctg
     1741 gtgcctcaat acattattaa ttttagcgtc tatcggttga actacgctgt ttcttgaagg
     1801 cagtgcagga tataattgcc ccagtggagt tcttagtttc cctgttcggg ccgggcaatt
     1861 ttatagatgc cgttggattg aagcttttgt tcccggtagg ccttaatgtc acggatcatc
     1921 ttacgggtga aaacattggc tccgttacta ttcaggtgtt gtgaattata gaaaaaatcc
     1981 ttatccaggc acatactgtc tttcgaataa tcaaagaagg gaatctggta tttacgggca
     2041 aagctgtcat aaatggcaaa cacctctttt ctgtttgata caaattgctg gccgcctata
     2101 tattcaggag aataaaccat aatgatctcg gttttcgtag ccctggcttc acgcaggaac
     2161 tggtcaaaaa gccggatagt agcactgtca aaatgctgca catatttttt ctgaacggcc
     2221 atcgcccggt ccaggtcatt attccagctt aaatcctgtc cgctatagcc cttataacgg
     2281 tcaatattat tcaatgaggg cttgatcata atcttcagcg cattttttaa agcatcctgc
     2341 tggccggcat accggatcaa tggaagttta taatcataga aatcgaatcc aaaatacgtt
     2401 ttggtggcat ccagaatttc cttgttatcc aggtagggga ggaattgttc cggattatac
     2461 aggtctttct ttttatcaaa agtaaaattg tctaaagaga ggatgatggt gcccgggggc
     2521 ttgttatatc ttaacaggag gtcatgccgg agatactgca tatagaagtt gtgcccgtct
     2581 atacccaggt tataagcgtc cttgccggtg ctgtcttcaa taatccgggg attcatctgc
     2641 agccaggccc gggacgatcc atagataacg atttcggaat ttacttttcc atcaagaata
     2701 tcattccata ccgcataatc accggttttt gaagtcttca gggtccggtt gatgtaacgg
     2761 tctgccacaa aagccaacaa caccagtggt gcaaggaaaa tgaatagctg gagtagaaac
     2821 tttttcatga tcagaactgg aaataaataa attgctgggt gtttccttta aaaacaaaca
     2881 gcaagagaat aattaaataa tatacactcc atcggaaaat acggttatac tttaccccga
     2941 atttttccaa agcatacctg gattccctgc cgtaccattc cgtcagaata aaggcagcta
     3001 acagaaaata aagcggacgg ggtataaatt catcggactg ttcaaagaaa tcagtggtga
     3061 aaatgccccc tatataatgg aaggcatgtg aaagagtatc tgcccggaaa aatatccagg
     3121 cgaaaagggt cagggtaaaa gtgagcagaa tggaaagcag gtcttttatt cccgggaaaa
     3181 tgcggccttt tgccacaata tccaggttat tacggttggt attcattaat accgacggca
     3241 tgataaaaag ggcattcaga aaaccccaga caataaaagt ccagttggca ccatgccaga
     3301 atccgctgac caggaaaatg ataaaagtgt ttcgcacttt catccatttg ccgcccttgc
     3361 tgccacccag cgggatgtac agatagtcgc ggaaccagct ggaaagggaa atatgccagc
     3421 gtctccagaa ttctgcgata tcacgcgaga agtaggggaa agcaaaattt cttaaaagat
     3481 caattccgaa cagtcgggcc gtacccagtg ctatatccga ataaccagag aagtcgccat
     3541 atatctggaa agcgaataag actgctccca ggaccaggat gcctccatga taactttcac
     3601 tgttattgaa aatcatgttg gcatattcgg cgcaattgtc tgcaatcaca atttttttga
     3661 acagccccca caggatctgc cgtagcccat ccactgcttt ggcaatttca aatttccttt
     3721 ctttgataat ctggggtaag aggtgggtgg ctctttcaat gggtccggca accagcaggg
     3781 gaaaaaagct gacaaagacc gaataatcaa caaagttttt ttccggttta atgcgcttat
     3841 aatagatatc cagtacgtag gagataccat ggaaggtgta aaaagaaata cctacgggca
     3901 ggattacact gatggtggcg taattcatct taatcccggc aagtgccagt ccgtctgcaa
     3961 aagatgatac gaaaaagtta tagtacttaa atacacccag gaagcccagg tttatcacta
     4021 tactgagcca catccaatac cttgatttct ttttggtagc actttcatgt atccggatac
     4081 ccgtataata atcaagcaaa gtggaaaaaa taaggaggaa aagaaaccgc cagtcccagc
     4141 aggcatagaa aaagtagctg gctaccagca gcaatatatt ttgtagcctg acttttcctt
     4201 tagtggcaaa ccaataaaga agaaatacca cgggtaaaaa aatggcaaaa cttatggaat
     4261 tgaaaagcat gaaataaaat aattctgttc tagatcaggt taatggagtt ttaaagggta
     4321 ttgtatttct gcttttccaa caaagggcca ggccctgcag gatcacgaat gctgaagttg
     4381 aattttctga tttgataccc tgtaatctta aaaccgtatt taaaaatagg ggctgtttga
     4441 caaatcgtga tgatatgaaa aacaacttat tatgaagttt ggtgtaaaag aaaacggttc
     4501 ttttgggctt ttagtacgtc ctaccggaaa tactataaag tcagaatatt ctcgtaacgt
     4561 gtgaaggtag tacataattt gggcaaatgc aagcttgccg gaagggtaat tttgacttag
     4621 atcaattttt attatattaa aaaatgtaat atgatataaa aatcaactat ccctcgagat
     4681 ggatggaaaa tacaatcatc ctggactgga ttttatcaaa catgctggaa aaacgcaggt
     4741 tttcatcgcg ggcgtggata ttcctcaggc cattactgat ttttatttca ggggagaata
     4801 taaagctggg aaaaaagaaa ttaaacccga taccgaattc cggaccgaaa tcatgtttct
     4861 ggatttttat cagttcttcc gctttcttgg cccgggcatt actggccata tcaatatccg
     4921 ctttaaagcc agccagcgta tataccctga aattaccaat ccggtcactt tggaatttta
     4981 tctggatggg gaaagacata atcaccgact ccactttttt ggtcacctcc gtattgccgt
     5041 caaaatccgg gtattttaac ttgtagaata tattccgctc aataaacatg agctgggggt
     5101 taaaacggac ctggaagcgg tcggaaatcc ggccggttgc tgataaacca agcgtgagtc
     5161 cgcctgaatt aatcggctcg ccaatcaaaa cactatcaga ttgcaggaaa cggggatgcc
     5221 attctgtatg gaaccgggcc agattgacgc ctatggtaat accgaaatag taaggcttgc
     5281 tgtcatggtc aaacatattc atctccactt taccaaattg ggcattggca gatgaagaaa
     5341 ccaacattaa accgttcagc agtacgggta ttatcaggga cctgattaaa cttttcttcc
     5401 ggagtatatg caacatatgc caaaggtcag cggctgaaac tctgtttcgg agtatcccac
     5461 tttttttaaa atgtcagtaa ataaatggcg atccggaaaa gcattggctg attcattgag
     5521 gtattcataa gcctccttat tctgcctgaa ccagcgggct acctgggggg ctattattcc
     5581 catatacagg ttatacaatc cttttaccac tttgcggcgg ggtttagaaa attccaacac
     5641 aatcagctgt cccccgggct ttaacacccg ctttatttca gtaaggcctt tctcgaggtt
     5701 ttcaaagttc cgtactccaa atgccaccat cacgccatca aacgtattat cagcaaattt
     5761 tattgtttcc gcatccccgg tatgtaactg gattttttct ccaagtccct ctttttcaat
     5821 cttttttctt ccgtattcca acattccttc cgaaatgtcg attccggtaa tctgcctggg
     5881 ttttaagatg gaatatgcca ttagcgccat gtccgcagtt ccggtagcca catccagcag
     5941 atgctgcggc tggtcctttt tgaacatatt aattgctttt ttccgccagc tgatgtcaat
     6001 ccgggcagat aaaaagcgat tcatcaggtc gtaccggccg gcaatccggt caaacatatc
     6061 ggctacctgc tcttttttgg tcttatcaga tcctttaaaa ggaatgatat tatcgtgggg
     6121 gagggggttt gacatggggg gcaaaggtag agagtttagg ggtttagtcg tttagtcgtt
     6181 tagggttttc ttaga
//
LOCUS       DAOPMW010000179         6194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14551_length_6194_cov_9.800782, whole genome shotgun sequence.
ACCESSION   DAOPMW010000179 DAOPMW010000000
VERSION     DAOPMW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14551_length_6194_cov_9.800782"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..407
                     /locus_tag="PLU11_09260"
     CDS             42..407
                     /locus_tag="PLU11_09260"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPN59350.1"
                     /translation="MSEEFNKFESLAEQLKAYVNTRVSQVKLSLAEKLSNLAATMIAL
                     LLSALVFFLFITLLCVAGALLIGQWLGSLWLGFLIVSGFVLLIGLIIWLAKDRLFRLP
                     IMNALIEAMFENEDEDEKD"
     gene            394..753
                     /locus_tag="PLU11_09265"
     CDS             394..753
                     /locus_tag="PLU11_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59351.1"
                     /translation="MKKIKRIQDIEREKLRLKVQQLEQEKKLKDSWDGLKHDLKPGNF
                     LRNKLNEFTHSKPDESGLVTGLVNVGAAYLSRRLTGYAGNKIDSTIHTGIEKASERLK
                     TFLLRKKKKKRSSKAKE"
     gene            complement(786..1913)
                     /locus_tag="PLU11_09270"
     CDS             complement(786..1913)
                     /locus_tag="PLU11_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPN59352.1"
                     /translation="MPDFKILILGGYGQTGLALTRLLIQETTVSIVLAGRDITKAKLA
                     AEALNNQLSTERVGACYADASEASTLTSAFKGVGLILVTSSTATYTRTIAEAALNAGC
                     DYMDIHFGLSVYTQLQQLESKIREAGRCFISGGGFHPGMPAIMIRYGAMLFDEIHSAR
                     TAGLMKIDFSTYNASTSTRIEFVEELAGFNPLYYTKGKWKKMNMLTGNGMRTFDFGNS
                     YGNCRCSPLFFEELRPLPQQYSSLRETGFYIAGFNLVTDYFIFPFIMLCLKIVPRRFN
                     SLLAKLMVWSIKTFTRPPYGYVMKLEAEGLKNGREKNIQVWVRSMDPAFITAVPVVAC
                     LKQYLKQNTRAAGLQVQGQFAEPVLFIEDCRRMGLEVKLSE"
     gene            complement(2178..3905)
                     /locus_tag="PLU11_09275"
     CDS             complement(2178..3905)
                     /locus_tag="PLU11_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN59353.1"
                     /translation="MRFFLLITALFLFHQLPAQLFIKNTNVIDVEQKKILAGYSVLIV
                     DGKIAQVDKDKNFKQLPEGTTIIDGTGKYLSPGFTDAHVHFFQSGGLYARPDAIDLRK
                     IRPYAEEIKWTHGHMEDLLRRYLSAGITSVIDVGATNNFLTQKDSFASKTWSPLVRMT
                     GPLLTTYVPAPFKNLGNDAPFTEMLNEENTRKAVRDQLPLNTDFIKIWYIVTDQDVEK
                     GARKNLPLVQAAIDEAHKNNKRVAVHATERITAQLAVEAGADFLVHSVDDEVVSDAFV
                     QLLKQKKTVLCPTLVVMGHYGKVLGDHYHFSTDELNLANPETIGSVLDYPWPDTSLAK
                     IYIKSMDSPLQAAKGKTADSIMTINLQKLVNGGVTIATGTDAGNTGTQHASSYFIELN
                     AMQNAGMNNWQLLEASTINGAKALGQEKEWGSVAKNKMANLVLLWANPLDSIANWRKI
                     SQVIVKGVAHLPETLVNPSPEYLVQQQLNAYNAHNLDAFLEPYAEDVELYEFPGKLIM
                     KGKEAMRKDYEFLTKVPKLYCKVQKRIIQGNMVIDHEEVSGFGPKPLYAVAIYIVEFG
                     KITKVYFKQ"
     gene            complement(4097..4702)
                     /locus_tag="PLU11_09280"
     CDS             complement(4097..4702)
                     /locus_tag="PLU11_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPN59354.1"
                     /translation="MVSMINGNTITGFFVSIAVMNRRLYIFLAWMIFALYGALLTKNI
                     LFKHGNYRYYKSYFNSEYKHYSAKKGWKKANTTPFHTINMYYRNGRINSENAKYNLFG
                     NLIGFVPFGMLFPLLFRRMRHLILTTLAGFGLSLLYEYIQVKTGLGFFDVDDLLLNTA
                     GALAGYILFWLLMKTWKFTRNEYNNMPLDKFQEPRCQDAKE"
     gene            4751..5128
                     /locus_tag="PLU11_09285"
     CDS             4751..5128
                     /locus_tag="PLU11_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPN59355.1"
                     /translation="MNLLGNLVWLIFGGFLAALCYFFGGLVLCFTIIGIPWGLQCFKL
                     AGFILMPFGLKVVSDSSGSGCLSTLANIVWIICGGLASAIIHVVMGLLLSITIIGIPW
                     GRQHFKLVELSLMPFGKRIVAAD"
     gene            complement(5125..>6194)
                     /locus_tag="PLU11_09290"
     CDS             complement(5125..>6194)
                     /locus_tag="PLU11_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPN59356.1"
                     /translation="SLMLTTTFLLYGANGYSGELIARYASQYNLHPILAGRRKEAIEP
                     LAAKYGLPFRIFDVNDTTALLTALKEVKVVMNAAGPFDQTARQIVEACLQTGTHYLDI
                     NGDISIFELIKRFDDAAKKAGILLLPGAGFDVVPTDCLAMHLKNKMPDAVSLKLAFAT
                     LGGALSHGTATTMANKLGEGGAARRNGKIVHEPLGQRGMWVDFGPKKLFVMSIPWGDI
                     STAHFTTGIPDIECYTGISRKVYYFLKLQGLFNWALRTDFIRKALKAKINRLPAGPTD
                     TQRAKAIGYVWGEATNAAGEKITARLTGPEGYTITVHASLIITRKVLNGDFKTGYGTP
                     AGVWGEGLIYEIPGVIKPFNN"
BASE COUNT         1572 a         1417 c         1423 g         1782 t
ORIGIN      
        1 gaagaagtga tctgaccgga taaaagccta atcaacccat catgtcagaa gaatttaaca
       61 aattcgagtc actggccgaa caactcaaag cttacgtaaa caccagggta tcgcaggtaa
      121 agctgtcact agcggaaaag ctgtccaatc ttgccgcaac tatgatcgct ttgttgctat
      181 cagccctggt gttcttttta ttcattaccc tgctttgtgt ggcaggggcc ctgcttatcg
      241 gtcaatggtt gggcagttta tggctgggct tcctgattgt atcgggcttt gtattgctga
      301 tcggactcat catctggctg gccaaagacc gccttttccg tctgcccatt atgaatgcgc
      361 tgatcgaagc catgttcgaa aatgaggacg aagatgaaaa agattaaacg tatacaggat
      421 attgaacggg aaaaactgcg gttgaaagta cagcagctgg aacaggaaaa aaaactcaaa
      481 gattcctggg acggactgaa gcatgacctt aaacccggta actttctccg taacaaacta
      541 aatgaattta cacattcaaa gccggatgaa tcaggactgg tcaccggact ggtaaatgtg
      601 ggtgccgctt accttagccg ccgccttact ggctatgcgg gaaacaaaat tgatagtacc
      661 attcacacgg gtattgaaaa agcgagtgag cgtttaaaaa catttttatt gcggaaaaag
      721 aaaaaaaaga gatcatccaa agcaaaagaa taaacctgct gatcgccgga tatttccatt
      781 attcatcatt cactcaattt cacttccagt cccattcgcc ggcaatcctc aataaacagg
      841 actggttctg caaactgccc ctgtacctgc aatccggctg ccctggtatt ttgcttcagg
      901 tattgtttaa gacaagccac aaccggtaca gctgtgataa atgcgggatc catactacgg
      961 acccaaacct gaatgttttt ctcccggcca tttttcaggc cttcggcttc cagtttcata
     1021 acataaccat aaggcggacg ggtaaatgtt ttaatactcc agaccatcag ttttgccagg
     1081 aggctattga atctccgggg gactattttc aggcaaagca tgataaaagg aaaaatgaaa
     1141 taatcggtaa ccaggttaaa gccggcaata tagaagccgg tttccctgag tgaggaatat
     1201 tgctgaggca gtggccggag ttcttcaaag aaaagcgggg aacacctgca attgccatag
     1261 ctgtttccaa aatcaaatgt acgcatacca tttccggtca gcatattcat tttcttccat
     1321 ttcccttttg tataatacag tggattaaag ccggccagtt cttcaacaaa ttcaatccgg
     1381 gtggacgtgc tggcattata ggtactgaag tcaatcttca tcagccccgc tgtacgggca
     1441 ctatgaattt cgtcaaataa cattgcacca tagcggatca ttattgcagg catgccggga
     1501 tggaatccgc cgccacttat aaagcaacgg ccggcttctc tgatttttga ttccagttgt
     1561 tgaagttgcg tgtatacgga aagcccgaaa tgaatatcca tataatcaca gccggcattc
     1621 agcgctgctt ctgcaatagt acgggtatag gttgctgtac ttgaagttac caggataaga
     1681 ccaacccctt taaaagctga ggtaagagtg gatgcctctg acgcatcagc ataacaggca
     1741 cccacccgtt cagtactcaa ctgattgttt agtgcttctg ctgccagctt tgctttagtg
     1801 atatcccgcc cggccaatac aatggataca gttgtttcct gtatcaacaa cctggtcaag
     1861 gcgagtcctg tctgtccgta ccccccgagt ataagtattt taaagtcagg catggtggta
     1921 ttcttttata aaaataccgg gcctgtgaat gctgtacaat gatttttatt tgtctgcggc
     1981 agaaggattt aattacacgc agtcattctg tattcaccaa tccctcctga gatgcttcaa
     2041 tggagtagtt tgtctttttt tgaatattga tttctggttc ttctttggca tcttgcttgc
     2101 ccaccgtagc cctgcctgcc ggcaggcagg tttagcgaag gtggggtgtc ttggtttctt
     2161 gattctttgt atcccagcta ttgtttaaaa tacaccttgg ttattttccc aaactctact
     2221 atataaatcg ccactgcata aagtggttta ggcccgaatc ccgatacttc ctcatggtcg
     2281 atcaccatat tgccctggat gatccgtttc tgaaccttgc agtataactt aggtaccttg
     2341 gtgagaaatt cataatcctt tcgcatcgct tctttccctt tcatgatcag ttttccgggg
     2401 aattcgtata actccacatc ttccgcatag ggttcaagaa aagcatcgag gttgtgagca
     2461 ttataggcat tgagttgttg ctgtaccagg tattccggtg agggattcac cagtgtttcc
     2521 ggcaaatgtg ccactccttt cacaatcacc tggctgatct ttctccagtt ggcaatactg
     2581 tccagcggat tggcccacag gagaaccagg ttagccattt tatttttagc aacactgccc
     2641 cattcctttt cctgtcccag ggctttagcg ccattgatgg tactggcttc taacagctgc
     2701 cagttattca ttccggcgtt ttgcatcgca ttcaattcga tgaaataaga actggcatgt
     2761 tgggtacccg tattgccggc atcggtgccg gtggcaatag ttacgccgcc attaaccagt
     2821 ttctgcaggt ttatggtcat aatggaatct gccgtttttc cctttgcggc ctgcaaaggg
     2881 gaatccatgc ttttaatata gattttggcc aggctggtat ccggccaggg atagtctagc
     2941 accgagccga tggtttccgg attggcgaga tttaattcat cggtgctgaa atgataatga
     3001 tcgccgagta ctttgccgta atgtcccatc actacgaggg tcggacaaag cacggtcttc
     3061 ttttgtttca gtaattgaac aaaagcatct gacacgactt catcatccac actatgtacc
     3121 aggaaatccg cccccgcttc cacggccagt tgcgcggtga tgcgttcggt ggcatgcacg
     3181 gccacgcgtt tattattttt atgggcttcg tcgatcgcag cctgtaccaa cggaagattt
     3241 ttccgggctc ctttttccac atcctggtcg gtcacaatat accagatctt gataaaatcg
     3301 gtgtttaagg gtaactgatc cctgaccgct ttccgggtat tctcttcgtt gagcatttcg
     3361 gtaaagggtg catcattccc caggtttttg aaaggcgccg gcacataagt ggtcagcagg
     3421 gggccggtca tacgtaccag gggtgaccag gttttgctgg caaaggaatc tttttgtgta
     3481 aggaaattat ttgtcgcacc tacatcaatc actgatgtaa ttccggcgga gagataacga
     3541 cgcagcaggt cttccatatg cccgtgtgtc catttgatct cctcggcata gggccggatc
     3601 ttacgcaggt cgatcgcgtc cggccttgca taaagtccgc cactctggaa gaagtgaacg
     3661 tgtgcatccg taaatccggg actgaggtat ttgccggttc cgtcgatgat tgtggtgcct
     3721 tccggcagtt gtttgaaatt tttatcctta tccacctggg caatctttcc gtcaacaatc
     3781 aaaacggaat acccggccag tatctttttc tgctccacat cgatgacgtt ggtgtttttg
     3841 ataaataact gagcggggag ctgatggaaa aggaacaagg cggtgatcag gaggaagaaa
     3901 cgcatggttt ttgttttttt taaagatacc gagaaaccga gaaaccaaga ttcaagacac
     3961 caagatgcca agacgccaaa gaataatcaa gctacaaagc aaaaataagc accaaatcct
     4021 ccgccggatt ttctcaggag ggtttggtgc tttttgaggc tttataattg gaatttaatt
     4081 gattattgta atttgattat tctttggcgt cttggcatct tggttcttgg aatttatcta
     4141 aaggcatatt attgtattca ttcctggtaa acttccaggt cttcattagt aaccagaaca
     4201 aaatataccc cgccagcgct ccagccgtgt tgagtaataa atcatccaca tcaaaaaatc
     4261 caagtcctgt tttcacctga atgtattcat ataataaact cagtccgaat ccggccaggg
     4321 tggtgagtat taaatgccgc atgcgacgga agagcagcgg gaaaagcatg ccgaagggaa
     4381 caaaaccgat caggttgccg aacaggttgt atttcgcgtt ttccgaattg atccgcccgt
     4441 ttctgtaata catattgatg gtatggaagg gtgtcgtatt cgcttttttc catccttttt
     4501 tggcagagta atgtttgtat tcgctgttaa aataactttt gtaataccgg taattaccgt
     4561 gcttaaatag gatgtttttg gtcagcaatg ccccatataa agcaaagatc atccaggcca
     4621 ggaaaatata gaggcgacgg ttcataactg cgatactaac gaaaaaaccc gtaatggtat
     4681 tgccatttat catactgacc attcagtttt gtgtattagt cggtaatttg tacctgatta
     4741 aacaaacaat atgaatcttc ttggtaacct ggtctggctg atctttggcg gctttttagc
     4801 ggcactttgt tattttttcg gcggattggt gttatgcttt acgattatcg gtattccctg
     4861 gggacttcaa tgtttcaaac tcgccggatt tatattaatg ccctttggcc ttaaggttgt
     4921 gagcgattca tccggctccg gctgtctttc cacgttggct aatatcgtct ggattatttg
     4981 tgggggactg gcttctgcca ttattcatgt cgtaatgggt ttactcctca gtattaccat
     5041 catcggtatt ccctggggaa ggcagcattt taaactggtg gagttgtcat tgatgccatt
     5101 tggaaagcgt attgtggcgg ctgattaatt attgaatggt tttatcaccc ccggtatttc
     5161 gtatatcaac ccttcccccc aaacccccgc cggtgttcca taacccgttt taaaatctcc
     5221 gttgagtact ttacgggtaa taatcagaga agcatgaacg gttatcgtat agccttccgg
     5281 acccgtcaac cgggcggtta ttttttcccc ggctgcgttg gtcgcttctc cccacacata
     5341 gccgatggct ttagcacgct gcgtatcggt ggggcctgcc ggaagacggt taatcttcgc
     5401 tttaagcgct ttccggataa aatcagtgcg taaagcccaa ttgaagagtc cctgcaattt
     5461 taaaaagtaa tataccttac gggagatacc ggtatagcat tcaatatccg gtataccggt
     5521 ggtgaaatgt gccgtggaga tatcacccca gggaatactc ataacgaaga gtttctttgg
     5581 gccgaagtct acccacatcc cccgttggcc gagtggttca tgtacgatct ttccgtttct
     5641 gcgggcggct ccgccttccc ctagtttatt agccatggta gttgccgtgc catgagagag
     5701 ggcacccccc agggtggcaa aagcaagttt aagtgaaacc gcatcgggca tcttgttttt
     5761 taagtgcata gccaggcaat ccgtgggtac cacatcaaaa ccagcgccgg gtaataaaag
     5821 gatgccggct ttttttgccg catcatcaaa ccgttttatc aactcaaaaa tggaaatatc
     5881 tccattgata tcgagataat gcgtgccggt ctgcagacag gcttccacga tttgccgggc
     5941 cgtttgatca aaggggccag cggcattcat gaccactttt acttctttca atgcggttaa
     6001 taaagcggtt gtgtcattga catcaaagat gcggaaaggc aatccgtatt ttgccgccaa
     6061 tggttcaatg gcttcctttc ttcgccctgc cagaatgggg tgcaggttat actggctggc
     6121 ataacgggca atcagttccc cgctataacc attggctcca tagaggagga aggtagtggt
     6181 aagcattaag gaaa
//
LOCUS       DAOPMW010000180         6193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14553_length_6193_cov_5.471978, whole genome shotgun sequence.
ACCESSION   DAOPMW010000180 DAOPMW010000000
VERSION     DAOPMW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14553_length_6193_cov_5.471978"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLU11_09295"
     CDS             <1..441
                     /locus_tag="PLU11_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59357.1"
                     /translation="HRYQEVVPDQTIFFLSYAGNHNRGAQFTNLHNEINEKMHELFDE
                     LSHQTRFYYGRYDIKTTSIEDLKQGKNISILEFNGCGAEPNHIYDCGMPLWKAYSTLL
                     KHWKALYQISRYNHKNGTPYWSYRRGKNFLKESNKHFKLLEQYD"
     gene            587..1309
                     /locus_tag="PLU11_09300"
     CDS             587..1309
                     /locus_tag="PLU11_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPN59358.1"
                     /translation="MPPLLKIFFTGMLVSFLGSLPLGTLNIAAMQISISSGVVAAMMF
                     SVGSLIAEIIYVRISLVAMDWVRKQERILKALEWVTLLIVTALAIFSFYAALHPKVEK
                     NMVLDSPLPKILLGFIMSAVNPVQIPFWFGWSTVLFSKKILLPRNDHYNTYILGIGLG
                     TFMGNALFIFGGLLIASKINNNQHILNWVIGGIFALTALIQLWKILRKKDAVHKMEHP
                     EEVAKHLEEQMGRFGMDDDKIR"
     gene            1325..1822
                     /locus_tag="PLU11_09305"
     CDS             1325..1822
                     /locus_tag="PLU11_09305"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59359.1"
                     /translation="MSIANSFITELKQEAAITRKMLERVPMDKREWKPHEKSMSVGRL
                     ATHIAETIHWISVILGPDRFDFAANPAPVRHVAADTAELLSILDHNLEQAVSTLSTAT
                     GDDFEKDWIIVRGEQEIMRSPKKAAIRSWGISHQVHHRGQLSVFLRLLEVPVPGVYGP
                     SADER"
     gene            complement(1826..2350)
                     /locus_tag="PLU11_09310"
     CDS             complement(1826..2350)
                     /locus_tag="PLU11_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59360.1"
                     /translation="MQKSSYLVILLLLISVLSHSQQLFELGGEMLKSYGKGYGTTKVA
                     ARGENFNNKNSFSAGITWQLASKKAYSVSTGFGIFAGYRRAFGNNLKGSNPFGGGRIL
                     ISLEDFEGKTRQKSIMITPMLEAGYHFVFGKRIYTAPAVGFGYTIEMSQGFNSLNEDV
                     GKRFIPSLSAGYRF"
     gene            complement(2578..2907)
                     /locus_tag="PLU11_09315"
     CDS             complement(2578..2907)
                     /locus_tag="PLU11_09315"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HPN59361.1"
                     /translation="MQLTNFPFQSINWSSVPREVHAGETGTAWWQVQHMNEVRVRMVE
                     YSPGYKADHWCSKGHIILCLEGEMETELADGRIMKLSKGMCYFVGDDNEAHRTSTVNG
                     CRLYIVD"
     gene            complement(3012..4346)
                     /locus_tag="PLU11_09320"
     CDS             complement(3012..4346)
                     /locus_tag="PLU11_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPN59362.1"
                     /translation="MQIIQQILFILLSAVAIFLFTKKAREIARNIHLGRPENLSDNPS
                     QRWKNLLLLAFGQKKMFRNPLVAVMHFFVYAGFIIINVEVLEIVLDGITGQHRLFAAP
                     LGGLYTFLINSFEILALTVLVACVIFLVRRNVIKLQRFVSKDLDGWPRSDANYILITE
                     IVLMSLFLKMNAADTLLQMRGAEHYAAHPTGNFIISQFLHPMMNGFSDGTLITLERSC
                     WWMHIVGIFAFLNYLPYSKHLHIILAFPNAYFARLKPMGEMENMPSIQQEVLYAMQPE
                     LAPTGEQPVPQKFGAKDVLDLSWKNLMDAYSCTECGRCSAACPANQTGKLLSPRKIMM
                     DTRDRLEVVGKNITANKEFKEDGKSLLHDYITVEELRACTTCNACVQECPVSISPLDI
                     ILQLRRYLVMEESNTPQEWAAMFSNVENNFAPWKFSPEERDLWATELNDQQK"
     gene            complement(4959..5987)
                     /locus_tag="PLU11_09325"
     CDS             complement(4959..5987)
                     /locus_tag="PLU11_09325"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPN59363.1"
                     /translation="MFCLLMLVLLTILQVQVLAQDSTMVSDQLELDSGWKANPGVSGG
                     QFKNFSVEKWEPLLSPAQKKRRTWLIGGANVVGYGGTMIGLYHSWYKNYPQSKFHTFN
                     DWREWKQVDKVGHLYSAYIESYGSMKMWQWAGMDRKKRIWIGGMSGAVYQTVIEALDG
                     FSTGWGWSWGDFSANMLGSGSLVAQELAWDEQRIKLKFSFHKKNYNDPELNRRSDKLF
                     GNSLAERMLKDYNGQTYWASANLKSFFKNSRLPDWLSVSVGYGAEGLFGGTENIGKDE
                     NGNIIFNRPDIKRYRQWFLAPDIDLTKIKTNKKGLKFLFTVLSAFKFPTPSLEYGNGR
                     FRLHALHF"
BASE COUNT         1654 a         1438 c         1454 g         1647 t
ORIGIN      
        1 catcgttatc aggaagtggt tccggatcag accatttttt ttctgtccta tgccggtaac
       61 cacaaccgtg gagcacagtt taccaacctg cacaatgaga tcaatgagaa aatgcatgaa
      121 ctgtttgatg aactgagtca tcagacccgg ttctactatg gccgttatga tatcaagact
      181 acttctattg aggacctcaa gcagggaaag aatatcagca tcctggagtt caatggctgc
      241 ggggccgagc ccaatcatat ttatgattgc ggaatgcccc tctggaaagc ctattccaca
      301 ttactcaagc actggaaagc cctctaccag atcagccggt ataatcataa gaatggtacg
      361 ccgtactggt cgtacagaag agggaaaaat tttcttaagg agtctaacaa acatttcaaa
      421 ttattggagc aatatgattg agctagaagc gagaagctag aagcaagaag gactttaaag
      481 cagaaagttg ctgactgtaa agttacttcc ctcatcacgc ccccttctcg cttctcgctt
      541 cttgcttctc gctttttcgt ttctagcttc tagttatctt tacactatgc cgccactact
      601 caagatattc ttcaccggta tgctcgtcag ctttctcggc tccctgccgc tgggaacgtt
      661 gaatatagcg gccatgcaga tatctatatc gagtggggta gtggcggcga tgatgttttc
      721 ggtagggtcg ctgatcgcgg agattattta tgtgcggatt tccctggtgg ccatggactg
      781 ggtgcggaaa caggagcgga tactcaaggc actggagtgg gtaaccttat tgattgtaac
      841 cgcgctggcc atatttagtt tttatgcggc cctgcatccg aaagtggaga agaatatggt
      901 actggacagt ccgctaccaa aaattttact tggatttatc atgagtgccg ttaacccggt
      961 acagattcct ttctggtttg gctggagcac ggtattgttt tccaaaaaaa tcctgctgcc
     1021 ccgcaatgat cattataaca cctatatcct gggtatcggt cttggtacat ttatgggaaa
     1081 cgccttgttt attttcgggg ggctcctgat tgccagtaag atcaataata accagcatat
     1141 cctcaactgg gtaatcgggg gcatttttgc ccttactgcc ctgattcagt tatggaaaat
     1201 attgagaaag aaggatgcgg tgcataaaat ggaacatccg gaggaagtgg ccaaacacct
     1261 ggaagaacaa atggggcgtt ttggaatgga tgacgataaa atccgttaat ctaaatatac
     1321 agttatgtca attgccaatt cctttattac agagcttaaa caggaagctg caataacccg
     1381 taaaatgctg gaacgggtac ctatggataa acgagaatgg aagccacacg aaaagtccat
     1441 gtccgtaggt cgcctggcca cccatattgc tgaaacgatc cattggatca gtgttatcct
     1501 tgggccagac aggtttgatt tcgcagctaa tccagcccct gttcgtcatg tggcagcaga
     1561 tacggccgaa ttgctcagta tattggatca taacctggaa caggcggtca gcacgttaag
     1621 tactgcaact ggggatgact ttgagaagga ttggatcatc gttcggggtg agcaggaaat
     1681 catgcgtagt cctaaaaaag cggctattcg ttcctggggg atcagtcacc aggttcacca
     1741 ccgggggcaa ttatccgtat tcctgcgttt gctggaggta ccggtgccgg gtgtatatgg
     1801 ccccagtgcg gatgagcgtt gaggttcaga aacgataacc cgccgagagg gaaggtataa
     1861 atcgtttgcc gacatcctca ttcagtgaat taaaaccctg actcatttca atggtatacc
     1921 caaacccgac agcaggtgcg gtatagattc gttttccaaa tacaaaatga taaccggctt
     1981 caagcatcgg cgtgatcatg atacttttct ggcgggtctt tccctcgaaa tcttccaggg
     2041 aaatcaaaat ccggccacca ccaaaaggat tactgccttt caggttattg ccaaaagccc
     2101 tgcggtaacc agcaaaaatc ccgaatccgg tagatacaga ataagctttt tttgaagcca
     2161 gctgccaggt gattccagca ctgaagctgt tcttattgtt gaaattttct cccctggcgg
     2221 ccactttggt ggtgccatag cccttgccat aggatttcag catttcacct cccagttcaa
     2281 acagttgctg ggagtgactc aatacggata tgagtaaaag caggattact aagtagctgg
     2341 atttttgcat atgaccataa cgtttttgag gtgaaaatat tataattagc caatgtgcca
     2401 atatgccaat gtgccaattt aggaagagac tcaaagatca atcagtcatt tcaataggat
     2461 gtgatgaaag ccattaaaaa gccgatgttc ggtttttttg ataataaatt caggaggtca
     2521 attggctaat tggcacattg gcatatttgc acattggcta attggcacat tagaaactta
     2581 gtcaacaata tacaaccgac atccgttaac agtggaagtc cggtgtgctt cattgtcatc
     2641 ccccacaaaa taacacatcc ccttgctcag tttcattatc cgtccgtccg ccagttccgt
     2701 ctccatttca ccttccaggc aaagaataat atgtcctttg ctgcaccagt gatcggcttt
     2761 gtagcccgga ctgtattcca ccatccgcac ccgtacctca ttcatgtgct gtacctgcca
     2821 ccaggctgta ccggtttcac ctgcgtgtac ttcgcggggt actgatgacc agttaataga
     2881 ttgaaaaggg aagttggtta gctgcatgtt cgtgtgttta ataaatgaaa attgagaatt
     2941 gagcattgaa tattgaacat tgctgttctt agaacttcaa ttttcaatat tcattacgca
     3001 atgttcaatt atcatttttg ttggtcattg agctcagttg cccacaggtc tctctcctcc
     3061 ggactaaact tccacggcgc aaaattattc tcgacattgc tgaacatggc cgcccattcc
     3121 tgcggggtat tgctttcttc cataactagg tagcgacgga gctgaaggat gatatcgagc
     3181 ggactgatgc ttaccgggca ttcctgcaca caggcattac aggtggtgca ggcgcggagt
     3241 tcttccaccg tgatataatc gtgtaaaagg gatttgccgt cttctttgaa ttctttgttc
     3301 gctgtgatat tttttcccac tacttccagc cggtcacggg tatccatcat gatcttgcgg
     3361 ggggagagga gtttgccggt ctggttggcc ggacaggctg ccgaacagcg accacattcg
     3421 gtacagctat atgcatccat caggttcttc cagctgaggt ccagcacatc tttggcgcca
     3481 aatttctgag gcaccggttg ttcgccggtg ggggccagtt cgggctgcat agcatataaa
     3541 acctcttgtt gaatcgaggg catattctcc atttctccca taggcttcag tcgcgcaaaa
     3601 taagcattcg ggaaagccag gatgatatgc aggtgtttgg aataggggag atagttcagg
     3661 aaggcaaaga tcccgacaat atgcatccac cagcaactgc gttccagggt aatcagggtg
     3721 ccatcactaa agccattcat catcggatgc aggaattggg aaatgataaa attaccagtg
     3781 ggatgagccg cataatgttc agcgccgcgc atttgtagta acgtatctgc tgcattcatt
     3841 tttaaaaaca gcgacattaa aacgatttca gtaatcaata tataattggc gtcactgcgg
     3901 ggccatccat ccagatcttt gcttacaaaa cgctgcagtt tgatcacatt cctgcgaacc
     3961 aggaaaatca cacaggccac cagtacggtc agggccagga tttcaaaact gttgatcaga
     4021 aaagtataga gcccccctaa tggagcggca aataagcggt gctggccggt gatgccgtcg
     4081 agtacaatct ccagcacttc tacattgata attataaatc cggcatagac aaaaaagtgc
     4141 atgactgcca ctaaaggatt ccggaacatt ttcttttgac cgaaggccag gaggagcagg
     4201 tttttccaac gctgggaggg gttatcgctg aggttctccg gtcgtccgag atggatatta
     4261 cgggcgatct cacgggcctt tttggtaaac aaaaatatag ccactgcgct gaggaggatg
     4321 aaaagtatct gttgaatgat ttgcatagcc gttgattacg ctgctaattt aagggatatg
     4381 cgccacggaa tgaacaagtg ttagccataa agattcgcct gcggcaaata ggggggccac
     4441 gaaaacacac aaaagcacac aaataataca gaaaggcagg ccacggataa cacggatatg
     4501 cacggatttt gattgcctgc ggcaataggg tggccactaa aacacacgaa ggggcacgaa
     4561 aaatacaaaa tacagaaagg caagccacgg atcacacgga cctgcctgcc ggcaggcagg
     4621 ttaacacgga ttatccctgc gggatggaat tttgccggaa agacgggaag aacgggaaga
     4681 gattttgaca gaggaaaaaa tatcccgcaa agcttataag actattcgat acaggtagcg
     4741 ttttttaaat gttcggacag tcttttattc tttgcgggat aatagcttaa aaggggcaga
     4801 gtcaatccgt gcttatccgt gtgatccgtg gctaaaacct gccgccaggc aggttcctgg
     4861 ctgttccttt cgtgtccctt cgtgtgtttt agtggccacc caattttgcc gcaggcaaag
     4921 ccattcatgg ccatcatagc tgcgaagcgg atatgacttc aaaagtggag cgcatgcaac
     4981 ctgaacctgc cgttaccata ttcgagggag ggcgttggga atttaaaggc actgagtacg
     5041 gtgaagagga atttcagccc ttttttattc gtctttattt tggtgagatc aatatccgga
     5101 gcgaggaacc attgccggta ccgtttgata tccggccggt tgaaaataat attgccattc
     5161 tcatccttcc cgatattttc ggtgccgcca aatagccctt cagctccata acccacggac
     5221 accgataacc agtcaggcag ccggctgttt ttaaagaagg atttcaggtt ggcacttgcc
     5281 caataggtct ggccattata atctttcagc atccgttccg ccagtgaatt accaaagagt
     5341 ttatcgctcc gccgattcag ttccggatca ttgtagtttt ttttgtggaa ggagaattta
     5401 agtttaatcc gctgctcatc ccaggccagt tcctgggcca ccagggagcc actgccgagc
     5461 atattggccg aaaaatcacc ccagctccag ccccagccgg tactgaagcc atccagcgct
     5521 tcgatcactg tctggtatac ggccccgctc ataccgccaa tccatatccg ctttttgcgg
     5581 tccatcccgg cccattgcca cattttcata ctgccatagc tttctatata ggcactatag
     5641 agatgaccga ccttgtccac ctgtttccac tccctccaat cgttaaacgt atggaattta
     5701 ctctgcggat agtttttata ccaggaatga taaagcccga tcatggtgcc tccatagccc
     5761 actacattgg ccccgccgat cagccaggtt cttctttttt tctgtgccgg gctgagtaga
     5821 ggttcccatt tttcaacgga aaaattctta aactggccac ctgatactcc cgggttcgct
     5881 ttccagccgc tatccagttc cagttggtct gaaaccatag tgctgtcctg ggctaaaacc
     5941 tgcacctgta aaatggtcag gagcaccagc atcagaagac aaaacatggg ttttcccggt
     6001 aaaggggaga aggatctcct gctaattttt acgactaccg ggctcatgca acaaaaatac
     6061 gttttagggg tgcttttcag tttttaaaaa gctaaaattc aataactttc cattttataa
     6121 tcaaacctta taacgatatg gacgccacaa tccaagcaaa gatcgatacc tggctgagcg
     6181 gaaattttga tga
//
LOCUS       DAOPMW010000181         6178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14608_length_6178_cov_9.609668, whole genome shotgun sequence.
ACCESSION   DAOPMW010000181 DAOPMW010000000
VERSION     DAOPMW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14608_length_6178_cov_9.609668"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLU11_09330"
     CDS             complement(<1..376)
                     /locus_tag="PLU11_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPN59364.1"
                     /translation="MKKHTLILLLSLVLCTNQVLAQTRTAWVAAKSGLSIREKPAIGA
                     KVLDKIPYGTKISILNTDEELVAVVTEGMHGFWEKVTFNNKTGYIVGSYLLPIAPPKL
                     ATVKEMADYFKQVYTPFGNKLVI"
     gene            complement(387..1226)
                     /locus_tag="PLU11_09335"
     CDS             complement(387..1226)
                     /locus_tag="PLU11_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPN59365.1"
                     /translation="MWSNLLHYFQNLEQRPLERLAILVGGLLFFWIIEGAIPLIPLRY
                     KKNKLRHAGVNFFFTVLHLILHTGLALIIVKLSDWCLSQQFGLVYWFNTGVLVTIIIG
                     VLALDFSSWLVHLIMHKNKFLWRFHLIHHSDTNVDVTTGLRHHPGDSLLRGIFFMLLI
                     LISGAPMYAVMIYQTLVVLATAFTHANINLPKGLDKALSYILISPNMHKVHHHWKQPY
                     TDSNYGAVFSIWDRLLGTFSFLDTKELKYGLDRYYPAEKDENVMSLLVSPFKKQDDSV
                     MNS"
     gene            1337..1798
                     /gene="tnpA"
                     /locus_tag="PLU11_09340"
     CDS             1337..1798
                     /gene="tnpA"
                     /locus_tag="PLU11_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPN59366.1"
                     /translation="MAGTFTQLYVQAVFAVRGRENLISPVWADRLYSYMGGIINAKKQ
                     KPIIINGMPDHVHVFFGLRPGMSVSDIVRDIKSSSTNFINENRFVKGKFFWQEGFGAF
                     SYSQSHIDRVYQYIKNQQEHHKKRSFRDEYKELLNRFQVEHKDQYLFEFYD"
     gene            complement(2530..2943)
                     /locus_tag="PLU11_09345"
     CDS             complement(2530..2943)
                     /locus_tag="PLU11_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPN59367.1"
                     /translation="MSDFTVINAYNNYVEAHIARGVLEEEGIESWLKDENTVTIDPIL
                     TNAVGGIKLMVESTQAQRAVDILVRLRQENRAAVACPKCGSHNIEEVSTPRKASNWLT
                     ALLTFGLGDYAIAPDKVNHCFDCGHEFEIEGKGEE"
     gene            complement(2946..3713)
                     /locus_tag="PLU11_09350"
     CDS             complement(2946..3713)
                     /locus_tag="PLU11_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HPN59368.1"
                     /translation="MQYKNNGSFHLPAALMLLAVFTLSSCKDKKSGSAETENEAIEKP
                     TDTPGANNQPDYRLGSFVGAFGENKITLLISSIKADSVQGRSIVAGNDRPFAGTIKKE
                     NTLWKIHAAEPGDHKDDGVFDLVMDPAKADAVMGTFTANDAKRPAKTFALDRKKFSYD
                     PAVGNYPTASQRLLTAEDVENEMKEDLEFMRNEIFARHGFCFNKKHLRQQFELQDWYV
                     PNTVDIKGFLTEIEKKNIALIKRYEKYAEEYGDEYGR"
     gene            3722..4378
                     /locus_tag="PLU11_09355"
     CDS             3722..4378
                     /locus_tag="PLU11_09355"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HPN59369.1"
                     /translation="MYLKIRQIISRTNIINASLLLGLAFFTWLMATITVAYLPYNTDV
                     GFLRIKQQYITIDEWRIAFFIHVYASMWVLLAGFTQFSRSLKQHYPRLHRGMGYVYVA
                     DVLFITGPAGLLMGFYANGGLSSRIAFVLLAVLWIYFTTMALVKAKQKDFTAHRNYMI
                     RSYALTLSAITLRAWKYAITNTVELPPMDVYRVVAWVGWGLNLIIAEWVIARKMIINK
                     "
     gene            complement(5009..6124)
                     /gene="dnaN"
                     /locus_tag="PLU11_09360"
     CDS             complement(5009..6124)
                     /gene="dnaN"
                     /locus_tag="PLU11_09360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPN59370.1"
                     /translation="MKFIASSSSLLKQLQHISGVINSNTVLPILEDFLFEVEKNKLTV
                     VATDLETVMRVQLEIEAKDSGKVCIPAKILMDSLKNIPDQPLTFNIDKNFAIEITSDN
                     GKYKVMGENPDNFPKEPTADDTTSFTMMASALVTAINKTLFATSNDDLRPAMTGVFFE
                     LDKKGLQCVATDAHRLVRYKRTDVACPKTDSFIAPRKPLNLLKSAIPSTDEEITVSYN
                     SNHLFVKHGTTQMSCRLIDARFPDYKVVIPADNPYRLTVSKSDFQSALRRVSVFSNKS
                     TNQVALSISGSELQLAAQDVDFSFEGNERMKCQYNGEDLMIAFNAKFLIEMLNATDSE
                     EVNMELSTPTKAGLIKPTDQDENEDLLMLVMPLMLNQ"
BASE COUNT         1601 a         1353 c         1552 g         1672 t
ORIGIN      
        1 taatcaccag tttattcccg aagggcgtgt agacctgttt gaagtaatcc gccatttctt
       61 ttactgtggc cagttttggt ggagctatag gtaaaagata agaacccaca atataaccgg
      121 tcttattatt gaatgtgact ttttcccaaa agccatgcat tccttcggtc accaccgcta
      181 ccaactcctc atccgtatta aggatcgata ttttagttcc gtaaggaatc ttgtccagca
      241 ctttggcacc aatagcgggt ttttccctta tgctgaggcc tgatttagca gccacccagg
      301 cagtacgggt ttgtgctaaa acctgattcg tgcatagaac aagagaaagg agaaggataa
      361 gagtatgttt cttcataata atagtatcag gagttcatga ccgaatcatc ttgtttttta
      421 aacggactta ccagcaagct cattacattt tcatcttttt cagccgggta atagcggtcc
      481 agtccatatt tcaattcttt tgtatcaaga aaggagaaag tgcccagcaa ccggtcccag
      541 atggaaaaca ccgcgccgta attgctgtcg gtatagggtt gtttccaatg gtgatgtact
      601 ttatgcatat tgggggagat gaggatataa ctaagcgcct tgtccagtcc cttgggcaaa
      661 ttgatattgg catgcgtaaa ggctgtagcc agtaccacca gggtctggta gatcatcacc
      721 gcatacatgg gggcgccgga gatgaggatg aggagcataa agaagatccc ccgcagtaaa
      781 ctatcacccg ggtgatggcg aagaccggtg gtcacatcca cattggtatc gctgtggtgt
      841 atcaggtgga agcgccagag gaatttgttc ttatgcatga tcaggtgtac cagccagctg
      901 ctgaagtcga gggcgagtac gccgattata atagtaacca gtaccccggt attaaaccag
      961 tacaccaatc cgaattgctg actgaggcac cagtcggata gtttcacgat aatcagcgcc
     1021 agtccggtat gcaggatcag gtgcagtacg gtgaagaaga agttcacgcc ggcatggcgt
     1081 agtttattct ttttataccg cagggggatc agcgggatgg cgccttcaat gatccagaag
     1141 aaaagcagtc cgcccacgag gatcgccagc cgttccagcg gccgttgttc caggttttgg
     1201 aaatagtgca acaggttgct ccacatagca agagatttcg ggaaaagata cgaaggaaaa
     1261 tgggtttttt cccaaggtgg ggttgccgcg tccggttaat ttgccgggac cacaggtagt
     1321 gggaaaatca aatagtatgg caggaacatt cacacagtta tatgtgcagg cggtgtttgc
     1381 ggtgaggggg agggagaatt tgatcagtcc ggtatgggca gatcgtttgt atagttatat
     1441 gggcggcatt atcaatgcca agaagcagaa gccaattatt attaatggca tgcccgatca
     1501 tgtgcatgtg ttttttgggt tacgtcccgg aatgagtgtt tcggatatag tcagagatat
     1561 taaaagttca tcgacgaatt ttataaatga aaacaggttt gtgaaaggga agtttttctg
     1621 gcaagagggt tttggtgctt tttcgtattc ccagtcacat attgatcggg tataccagta
     1681 tattaagaac cagcaggaac atcacaaaaa gagatcattc cgggacgaat acaaggaatt
     1741 attgaatcgt tttcaggttg aacataaaga ccaatatttg tttgaatttt atgattgata
     1801 ataatggagc gggaaaaatt tattgattcc aggaaacgta gggtcatttc ctaattttac
     1861 accaaataac tgtgtcacct ctataattgt gtcacccctt cggggctagg gggcatctcc
     1921 gatgtatgtg tttctataat tgtgccaccc cttcggggct agggggtatc accgaaatat
     1981 gcgtttctat aattgtgtca ccccttcggg gctagggggc atctccgatg tatgtgtttt
     2041 tataattgtg tcaccccttc ggggctttat ttagataacg ccaattaatt atcgaactcg
     2101 cccaaaaggg tgatataatt ataggaaatc gcacggcaaa gaacgattag ccccgaaggg
     2161 gtgatatagt tatagaacgg aaatgtgaaa atgcccacag agccccgaag gggtggcaca
     2221 attataggaa atcgcagggc agaaagcgat tagccccgaa ggggtgatat aattatagaa
     2281 cggaaatgtg aaaatgccca cagagccccg aaggggtggc acaattatag gaaatcgcag
     2341 ggcagaaagc gattagcccc gaaggggtga tataattata gaacggaaat gtgaaaatgc
     2401 ccacagagcc ccgaaggggt ggcacaatta taggaaatcg cacggcaaag aacgattagc
     2461 cccgaagggg tgacacaatt atatgaaaag gatcggaaaa ccttaatagg taacacctca
     2521 tatgaaaatt cattcctccc ccttcccctc aatctcaaac tcatgcccgc aatcaaaaca
     2581 atgattcacc ttatcggggg cgatggcata atctcccagg ccgaacgtga ggagcgcggt
     2641 gagccaattg ctggctttac ggggcgtgct gacttcttct atattatgcg aaccacattt
     2701 gggacaggca actgctgccc ggttctcctg gcgtaaccgg accagaatat caaccgcccg
     2761 ttgtgcctgg gtgctttcca ccatcagttt gataccccct actgcattgg taagaatcgg
     2821 atcgatggtc acggtatttt catcctttaa ccagctttca atcccttcct cttccaatac
     2881 accacgggca atatgtgctt ccacataatt attatacgca ttgatcactg taaaatcact
     2941 cataattacc tcccatattc atccccatac tcctccgcat atttctcata gcgtttgatc
     3001 agggcgatgt tctttttttc gatctccgtc aaaaatcctt ttatatccac tgtattgggc
     3061 acgtaccagt cctgcagttc gaactgctga cgcaggtgct ttttattaaa acaaaatcca
     3121 tggcgggcaa agatctcatt gcgcatgaat tcaaggtctt ccttcatttc attctccaca
     3181 tcttcggccg tgagcaatcg ctgggaggcc gtgggataat tacctaccgc cggatcataa
     3241 ctgaattttt tccgatcgag ggcaaaggtt ttggccggac gtttcgcatc attggcagta
     3301 aaggtaccca tgaccgcatc tgctttggcc ggatccatga ctagatcaaa caccccgtca
     3361 tctttatgat caccgggctc ggcggcatgt attttccata acgtattctc ttttttaata
     3421 gtgccagcga aaggacggtc attacccgcc acaatggatc ggccctgcac agagtcagct
     3481 ttgatggaac tgatcaggag ggtgattttg ttttcaccaa aggcacccac aaaggaacca
     3541 agccggtaat cgggttggtt attggcgccg ggtgtatccg tgggcttttc gattgcttcg
     3601 ttttccgttt cggcgctgcc tgattttttg tccttgcagg aagaaagggt gaagacggcc
     3661 agcaacatta aagcagcggg caggtggaag gaaccgttat ttttgtactg cataattgga
     3721 gttgtattta aaaataagac aaattatcag ccgcaccaat atcatcaacg catcgctcct
     3781 cctgggcctg gcctttttta cctggctaat ggccactata acggtggcct acctgcccta
     3841 taatacggat gtgggtttcc tgcggatcaa acaacagtat atcacgatcg atgaatggcg
     3901 gatcgctttc tttattcacg tatacgcaag tatgtgggtg ctgctggccg ggttcacgca
     3961 attttccaga tcacttaaac agcattatcc ccggttacat cgcggaatgg ggtacgtgta
     4021 tgtggcggat gtgttattca tcaccggtcc ggccggtttg ctgatgggct tttatgccaa
     4081 tggtggattg agttcccgga ttgcatttgt gctgctggcg gttttgtgga tctattttac
     4141 caccatggca ttagtaaaag caaagcagaa agattttacc gcccaccgta attatatgat
     4201 ccgtagttat gccctgactt tatcggctat taccctgcgg gcctggaaat acgccattac
     4261 caacaccgtt gaattacccc cgatggatgt gtatcgggtg gtggcgtggg ttgggtgggg
     4321 gttgaattta atcatcgctg agtgggtgat tgcccggaaa atgattatta ataaataatc
     4381 aatatttacg agacacgggc tttgcccttt gtcaaaggaa ttcaaccccg ttggggttgg
     4441 ggggcggtta acacgtcgaa ccccgggttg gcacccgggg ctatcattgt tcaacccttt
     4501 cagggttggc agcataagta atggttgaat agaaatcccg tttttatcgt aaggtattaa
     4561 tccttcaacc cctttcaggg ttggcagtat atgctgaaac ctgagcattg aagaatccag
     4621 tgctttggtt aatagaattc atgaatcaat aatccatttt cattgaggag tcgggggcaa
     4681 caattgatgt ttgaaactgt aaataaattc aattgtgtat cacgtctaac cccgaagggg
     4741 ttgaacaatg gtagcccccg gtgaaaccgg gggtaaagag caaaaaaacg accccacaac
     4801 cctgaagggg ttgaaccagt gaagtaggga aaagaaaaag cccccctttt tcaagagaga
     4861 ctttcaaagc tagattatat gaagtttacc gtattcaccc ttcggcacgc ctgcctgtcc
     4921 ggtaggcagg cctgcctgtc cggtaggcag gctcagggtg acatcggtgc ttgtcatgct
     4981 gagcttgccg aagcaagacg caatgcttct attgattcaa catcaacggc atcaccagca
     5041 tcaacagatc ctcgttctcg tcctggtcgg taggtttgat cagaccggcc ttggtgggcg
     5101 tggaaagttc catatttact tcttcgctgt cggtggcgtt gagcatttcg atgaggaact
     5161 tggcgttgaa ggcgatcatc aggtcctcgc cgttgtactg gcatttcatg cgctcattac
     5221 cttcaaaact gaagtccaca tcctgggcgg ccagttgcag ttcgctgcca ctgatactca
     5281 gggctacctg gttggtactc ttgttactga aaacgcttac acgacgcagg gcactctgga
     5341 aatcgctctt gctaacggtc aggcgataag gattgtcggc cgggatcacc actttatagt
     5401 cagggaaacg ggcatcaatc aagcggcaac tcatctgggt agtgccgtgt tttacaaaga
     5461 ggtgattgct gttatagctc acggtgattt cttcgtcggt ggaaggaatc gcggatttta
     5521 agaggttcag gggcttgcgc ggagcgatga aagagtccgt tttgggacag gctacatctg
     5581 ttcttttata gcgcaccagg cggtgggcat cggtagccac acactgcagg ccttttttgt
     5641 cgagttcgaa gaaaacgccg gtcatagcag gacgcaggtc atcattgctg gtggcaaaca
     5701 gggttttgtt aatggcagta accagagccg aagccatcat ggtaaaggag gtggtatcgt
     5761 cggcggtggg ctctttcggg aaattgtccg gattttcacc cattaccttg tacttaccat
     5821 tatcgctggt gatttcgatg gcaaaattct tgtcgatatt gaaggtcagg ggctggtcgg
     5881 ggatattttt cagcgaatcc atcaggattt tggccggaat acacacttta ccactgtctt
     5941 tggcttcaat ttccagttgc acccgcatta cggtctccag gtcggtggcc actacggtca
     6001 gtttgttttt ttccacttca aagaggaaat cttccaggat cggcaatacg gtgttggaat
     6061 tgattacacc agaaatatgc tgcaattgtt tcagcaggga tgaagaagag gctatgaatt
     6121 tcataatatg aattgaatgg ggcgaaacta ctgattttta acatacggca atcaattc
//
LOCUS       DAOPMW010000182         6158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14674_length_6158_cov_6.433885, whole genome shotgun sequence.
ACCESSION   DAOPMW010000182 DAOPMW010000000
VERSION     DAOPMW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14674_length_6158_cov_6.433885"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..943
                     /locus_tag="PLU11_09365"
     CDS             86..943
                     /locus_tag="PLU11_09365"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HPN59371.1"
                     /translation="MRCLNFSTRWICPVMILLLLSSCGTGKVRRIQSPPHYSFAQVFT
                     HKLEPKLKEISGLAWDKKLDEFIAHNDESGKLFYLDKETKLIKSELPFGEKGDYEEVV
                     VVNSVPYVLRSDGLILMISKDSTGKAIPVEMGKVGISGLNDFESMYFDPARNALVILC
                     KNCESDNKQVVSAYACNIDSTGFDPDPVFTISVDEVKTASPFKSGRLEPSAAAIHPVL
                     QKLFIVSSASSQLIITSLDGKLEGVYELGKKLFPQPEGITFKSNGDMYISNEGRAERA
                     TILRFPYRK"
     gene            complement(960..3326)
                     /locus_tag="PLU11_09370"
     CDS             complement(960..3326)
                     /locus_tag="PLU11_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59372.1"
                     /translation="MPEHHYTLPAFNSLLSFRPLVAALKKNIEEGNTGMKKLYGHVVK
                     ELESHPELMGTIHDLALLDPHHELIEELLSAVFPPTTANYMYGISYPFKNLAVYASPL
                     FKKIMIRKNSNEILVGNDPDHSLDKQRLRFAYGLILKKYFGFESPENSRVVYPVTDEK
                     TGLTRYMEMRLDGRFIDVSPVKGMPALPTSLLDPATNRLFTMEELMKMVPLEQFIFEG
                     LTVLRINDVTEQAVITEMKNFLLDFNGLPGSVSYTRLEKMIQDLIGLKDLTIGITPFF
                     RIQGHYIYSELHNSQSLLYRQCSNVTEKDEISDYSKILFRDNHQPVLYQVLDQKNTAH
                     IQCLHNYYRAGSRSIIICPLKQGKELLGLLEISSHEPGYLQPFHISKIEPAVPLFKIS
                     LEKSLEQLNSQVEELIKKKFTAVQPAVEWKFTEVSLNYLVSHYKQSDTRLERIVFEQV
                     YPFFGAIDVRNSSTARSKAVQADLLEQLELAAVVIRKAQQEQSFPILYELLNKIEKYK
                     SAVAETLRSNEEMAVYDFLQGQLLAVFNHLLETEPAVKVETETYFASLDPQRQYLYRH
                     RKEYEQSIARINETLARFVDREQQAAQKIYPHYFERFITDGLEFNMFMGQSISPRKKM
                     DEIYLRNLKMWQLTMMVKAARVTAQLEQELPMPLRTTQLILASNQPLTICFRTEERKF
                     DVDGVGNTRYEIIKKRIDKVHIRNSDERLTQPGMVAIVYTQAKDATEYKEYIEFLQHK
                     ELLQPGIESLDLEELQGVSGLKALRVAIRPDLPGTLAVIAKETKLVVE"
     gene            complement(3435..4367)
                     /locus_tag="PLU11_09375"
     CDS             complement(3435..4367)
                     /locus_tag="PLU11_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59373.1"
                     /translation="MKLYCIRMLMIPLLLTVIRSGAQGYYYYDDRHYEGPVLAELGVK
                     GGVMNALTDIGGQSGAGKNGLKDLNLAFSRPCFSIYALVLYQNTIGLRIHYAAGQVTA
                     ADSILKKDRISTHGRYERNLSFRSPVWELGLILEIHPLSIFNNNRTDKNPSRFSPYLL
                     AGIGFFSFNPQAEWEGRWFPLQPLHTEGQGFSMYPDRKPYSLRQMNWQTGAGLKYEIN
                     AWLNGRFEIMHRFLKTDYLDDVSRDSYVQPALFNRELPPALAGLARALADRRAEVDPG
                     HITNLSYQRGNPANKDAYFTAEIGVGIVLGRSRR"
     gene            complement(4506..6110)
                     /locus_tag="PLU11_09380"
     CDS             complement(4506..6110)
                     /locus_tag="PLU11_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPN59374.1"
                     /translation="MMNRRNFLQNTVPLAVTMPGLLNGFSFTANGAESPLARLLEQTL
                     TDTDHVFVLVQLSGGNDGLNMVIPLDIYGNYYNARTNVAIPQSQVLRLDGTDKSGLHP
                     AMTAMQNMFNEGKMAIVQSVGYDTPNFSHFHATDIWNSADTRRNRRERVKTGWMGRYL
                     ESEYPGYPEAFPNNNMPDPLAIQISNFPTLMAQGTIYSMGLSITNPEKIYTFANPFND
                     YPLNAAPANKELKFLRVISEKTKIYSDIIRTAYQRAATMAAYPTENGLAEQLKIVARL
                     IKGGLKTRVYTVTMGGFDTHKKQVNASDTTTGMHAKLMKELSGAISAFQDDLELMGLD
                     ERVMGMTYSEFGRRIKSNASLGTDHGAAAPLILFGKNAKKGILGTSPDIPADAQVVNN
                     IPFQYDFRSVYASVLEQWFCVKQPALDSIMLRNFQSLPLVTGPCGVADDPEDNNNAAD
                     KLILKMWPSPYTINATIQFSTNGGNTMIQQIDGLGRVVKVLTNQDYAAGTYNIDIYND
                     GLATGVYFLRLQNKSLQKVISVMKMP"
BASE COUNT         1540 a         1410 c         1495 g         1713 t
ORIGIN      
        1 actcctgggc ggctttcaac agtgctgacg gattttccat catgttataa tgataacttt
       61 tgataaattt aatgaaaatt agagaatgcg ttgtttaaat ttcagtaccc gttggatctg
      121 tccggtgatg atattgctgc tgctgagcag ctgtggtact ggtaaagtac gccggattca
      181 atccccgccc cattatagtt ttgcccaggt atttacccat aaactggaac ccaaactgaa
      241 agaaatatcc gggcttgcct gggataaaaa actggatgag tttattgcgc ataatgatga
      301 atccgggaaa ttgttttacc tggacaagga aaccaaactg atcaaaagtg aactgccctt
      361 cggggaaaaa ggggattatg aggaggtggt agttgtgaat agtgttcctt acgtattaag
      421 aagcgatgga ctgatactta tgatttcaaa agacagtacc ggcaaagcca tacccgtgga
      481 aatgggaaaa gtgggaatca gcggcctcaa cgattttgag tctatgtact ttgatccggc
      541 ccgtaatgcg ttggtgatac tttgcaagaa ctgcgagtca gataataaac aggtggtgag
      601 tgcctatgcc tgcaatatag attccacggg atttgatccg gaccccgttt ttactatatc
      661 cgtagatgaa gtgaagacgg cttccccatt taaaagcgga cggttagagc cttccgctgc
      721 ggctattcat ccggtattgc aaaaactctt tatagtttca tctgccagca gccagctgat
      781 cattaccagc ctggatggca aactggaagg agtctatgaa ttagggaaga aattatttcc
      841 gcaaccggaa ggcatcacct ttaaatctaa cggcgatatg tatatttcca atgaaggccg
      901 ggcggaaaga gcgaccattc tcaggttccc ctaccggaaa taaaaaaata atcaactgat
      961 tattcaacaa ccagctttgt ttcttttgct attaccgcca atgttccagg caggtctggc
     1021 cggatggcca cccgcagcgc ttttaagccg gagacaccct gtaattcttc caggtcgaga
     1081 ctttcgatac cgggctgcaa aagttcttta tgttgcagga attcaatgta ctctttgtat
     1141 tctgttgcgt ctttggcctg cgtgtataca atagcgacca tgccgggttg ggtgagccgt
     1201 tcatcgctgt tcctgatatg tactttgtca atccgctttt tgatgatctc atagcgggta
     1261 ttgcccaccc catccacatc gaatttccgt tcttcggtcc ggaaacaaat ggtgagtggc
     1321 tgattgctgg ccaggatcag ctgggtggtg cgtagcggca tgggtaactc ctgttccagc
     1381 tgtgcagtaa cccgggccgc ttttaccatc attgtcagtt gccacatttt caggttgcgg
     1441 agataaatct catccatctt tttccgcgga ctgatggatt gtcccataaa catattgaac
     1501 tcgagtccgt ccgtgataaa ccgttcaaaa tagtgcgggt agattttctg tgccgcctgt
     1561 tgttcccggt ctacaaaccg cgccagggtt tcattaatgc gggcaatact ctgttcatat
     1621 tctttgcggt gccggtataa atactgtcgc tggggatcca ggcttgcaaa ataagtttcc
     1681 gtttccactt ttaccgcagg ctcggtttcc agcaggtgat taaatacggc cagcagctga
     1741 ccttgcagaa aatcatacac ggccatttcc tcattggagc gaagggtttc tgcgacggct
     1801 gatttgtatt tttcaatttt attgagcagt tcatataaaa tcgggaagct ctgttcctgt
     1861 tgtgctttac gaatgaccac agcggccagt tccaactgtt ccaacaggtc ggcctgtacc
     1921 gctttggatc gcgcagtgga agaattacgt acatcgattg caccaaaaaa cggatagacc
     1981 tgttcaaata cgatccgttc caaccgggtg tccgactgct tgtaatgact gacgaggtaa
     2041 ttcagggaaa cctcggtgaa cttccattcc acggctggtt gtaccgcggt aaactttttc
     2101 ttaatcagtt cctccacctg gctgttcagt tgctccaggc ttttctcgag gctgatcttg
     2161 aataagggta cagcgggttc aatcttactg atatgaaagg gctgcaggta accaggttca
     2221 tgagaactga tctccaatag tcccagtaat tccttaccct gcttgagcgg acagatgata
     2281 atactccggc tgccggcccg gtaataatta tgaagacatt ggatgtgcgc cgtgtttttt
     2341 tgatccaata cctggtagag taccggctga tgattgtcgc ggaaaaggat tttactgtaa
     2401 tcactgattt cgtctttttc cgtgacgttg ctgcattgcc ggtacagcag actctggcta
     2461 ttgtgcagtt cagaataaat atagtgtccc tgtatgcgga agaagggggt aatgccaatc
     2521 gtcaggtctt tcagtccaat caggtcctga atcatttttt ccaaacgggt ataagataca
     2581 ctaccgggca gtccgttaaa atccagcagg aagtttttca tttcggtgat taccgcctgt
     2641 tcagtcacat cattgatccg taacaccgtc agtccttcga aaataaattg ttccaatgga
     2701 accattttca tcagttcctc catggtgaaa agccggttgg tcgccggatc gagcagactg
     2761 gtgggtaatg cgggcatacc ttttaccggc gatacatcaa tgaaacgtcc atccagccgc
     2821 atttccatat aacgggtcag cccggttttt tcatcggtta ccgggtaaac caccctggag
     2881 ttttccgggc tttcaaatcc gaaatatttt ttcagaatca ggccataggc aaagcgcagc
     2941 cgttgtttat ccagtgaatg atcggggtca ttgcctacca ggatttcatt actgtttttc
     3001 cggatcatta ttttcttgaa gaggggagaa gcatagactg ccaggttctt aaagggataa
     3061 gaaatcccat acatataatt ggcggtagtg ggcggaaaaa cggccgataa taattcttcg
     3121 atcagttcat gatgcggatc cagcagggcc aggtcatgga tcgtgcccat cagttccggg
     3181 tgggattcca gctcttttac cacatgcccg tacaattttt tcatacctgt gttaccctct
     3241 tcaatgttct ttttcagggc agctaccagt ggcctgaagg aaagcaggct gttaaatgct
     3301 ggaagtgtat aatggtgttc aggcatggtt gcagttcagg ataagcgaaa aagtgaattc
     3361 ccgggtgtaa agtattgggt atttcaaaga tacagtttca gtccatgtac tgcaaagcca
     3421 aatgcccgga tctttcaacg acggctccgg cccagcacga tccccacacc tatttctgct
     3481 gtaaaataag cgtccttatt cgcaggattg ccccgctggt aggaaagatt tgtgatatga
     3541 ccgggatcca cttctgcgcg gcggtcggcc aaagcccgcg ccagtcccgc caaagcgggt
     3601 ggaagttccc ggttgaaaag agcaggttgt acataactat cccggctcac atcatccagg
     3661 taatcggttt tcaggaaacg atgcatgatt tcaaagcgcc cgttgagcca tgcattgatt
     3721 tcatatttta atccggcacc ggtctgccaa ttcatttgac gaagtgaata gggtttccgg
     3781 tcaggataca tggaaaatcc ttgcccttcc gtatgcaagg gttgtaaggg gaaccatcgc
     3841 ccttcccact cagcttgtgg attaaaggag aagaaaccga ttcctgccag caggtacggc
     3901 gaaaaccgtg atgggttttt atcagttctg ttattattga atatagataa gggatgaatt
     3961 tccagaatga ggccaagctc ccagaccgga cttcgaaaac ttaagttccg ttcataacgg
     4021 ccatgggtag agatccggtc tttttttaaa atactgtcgg cggctgttac ctggccggcg
     4081 gcataatgta tacgaagtcc gatcgtattt tgataaagca ccaaagcata tatactgaaa
     4141 cagggacgac tgaatgccag gttgaggtcc ttcagcccgt ttttcccggc tcctgattgt
     4201 ccgccgatat cggtcagggc attcataacg ccgcctttta ctcccagttc ggctaacacc
     4261 ggaccctcat aatggcggtc atcataataa tagtagccct gtgcaccgga gcggatgacg
     4321 gttagcaata gcgggatcat taacatacgg atgcagtata gtttcatacc ggtaattttc
     4381 agtgataagg tgtattgata tttcaatgct acttaaaact attacaggta ttgggcagta
     4441 aaaagagaaa aacacccggt ttgggtgttt ttattatctg gaattatggg tgtatttctg
     4501 caggatcagg gcatcttcat tacgctgatc actttctgta acgacttatt ctgcagccgg
     4561 aggaaataaa ccccggtagc gagtccatca ttatagatat caatattata agtaccggct
     4621 gcataatcct ggttggtcag cactttcact acccggccta aaccatcgat ttgctggatc
     4681 atcgtattgc cgccattggt ggagaactgg atcgtggcat tgatcgtata gggactgggc
     4741 cacattttca gaatcagttt gtccgccgca ttgttgttgt cttccggatc atcggctact
     4801 ccacaggggc cggtaacgag gggtagtgac tggaaattac gaagcataat ggaatcaaga
     4861 gccggttgct tcacacagaa ccattgctcc agtacggatg catatacact gcggaaatca
     4921 tactggaacg gtatattatt gaccacctgg gcatcggccg ggatatccgg actggtaccg
     4981 agaattcctt tcttggcatt tttaccaaat aatatcagcg gggccgccgc accatggtcg
     5041 gtgccgagac tcgcattgga tttaatacga cgaccaaatt cagaataggt catccccatc
     5101 acccgctcat cgagccccat cagttccagg tcatcctgga aggcactgat ggcaccggat
     5161 aattccttca tcagtttagc atgcataccc gtagtcgtgt cactggcatt cacctgtttt
     5221 ttatgggtat caaatccacc catcgtcacg gtatacaccc ttgttttaag tccacccttt
     5281 atcagcctcg ccacgatctt taattgttcc gccagtccgt tttccgtagg ataagcggcc
     5341 atagtggcgg cccgctgata agcggtacgg ataatatcgg aatagatctt tgttttttca
     5401 gagatcaccc ggagaaactt gagttcttta ttggccggag cagcgttgag cgggtagtca
     5461 ttgaacggat tagcgaacgt atagatcttc tccggattgg taatggataa gcccattgaa
     5521 taaatcgttc cctgtgccat cagtgtggga aaattgctga tctggatggc cagcggatcg
     5581 ggcatattgt tgttaggaaa tgcttcgggg tatccggggt attcgctttc caggtagcgg
     5641 cccatccagc cggtcttcac ccgttccctc ctgttacgcc gggtatccgc actgttccag
     5701 atatccgtgg catggaaatg ggaaaagttg ggggtgtcat aacctaccga ttgcacaatc
     5761 gccatcttgc cttcattgaa catattctgc atggccgtca tagcggggtg cagaccggat
     5821 ttatccgtgc catcgaggcg taatacctgg ctttgcggaa tagccacatt ggtacgggca
     5881 ttataataat tgccatagat atcaagcggg atcaccatat tcagtccgtc gttgcctccg
     5941 gataattgta ccagtacaaa gacatggtcc gtgtcagtga gtgtttgttc caataaacgg
     6001 gccaatggac tttccgctcc attggcggta aaggaaaatc cgttgagcag tccgggcatt
     6061 gtgacagcga ggggaacggt attttgtaag aagtttctgc ggttcatcat ggttttgtgt
     6121 ttacatggat aaatcagtca tcagcataaa tggaattc
//
LOCUS       DAOPMW010000183         6151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14699_length_6151_cov_4.683563, whole genome shotgun sequence.
ACCESSION   DAOPMW010000183 DAOPMW010000000
VERSION     DAOPMW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14699_length_6151_cov_4.683563"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..224
                     /locus_tag="PLU11_09385"
     CDS             60..224
                     /locus_tag="PLU11_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59375.1"
                     /translation="MGVIDFTTTQQQTIRITPLVGTQNNNNLDMIHFIPVDEEQVLPR
                     FKPDGTKLYL"
     gene            309..1745
                     /locus_tag="PLU11_09390"
     CDS             309..1745
                     /locus_tag="PLU11_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN59376.1"
                     /translation="MNSRKWMIACALMTAGFFGSGCNKTIDDQTAITDPSLNKDLMEL
                     ISEKADLSTFAGYLKQTGYDAVIASSRTYTVFAPGNAELATLDAAVKNDAAKLKQFIG
                     NHITTQVYSTKDVTSTTRILMANGKYLSMNGTAIGASLITTKDQYAANGLLQQVDKML
                     PALENTWDFVAGSQSPANQKAFMMSLFRNVFDATNAIVIGINQTTGDPIYQAGTDSIF
                     TNLFWNKVADLRDEKKQFTLFMMQDAAWDADQARFAPYYVTNTADSNKLVTGWNVLRD
                     LAVDTLYEPGSIPDTITSAFGTRVPIERSAIVNTIRTSNGIVYIMSRINVQPSSKLKT
                     IVIEGENYNSTSHDRRGNTYFRERYNPVTGQDFRDVEVFNHGVALFNIRYQVEEIPSI
                     KYKAYWVAVNDFQTAAFNQRLAPGIATATTFPYVAVPVSNFNEVYLGEITLAQYQPVY
                     NFYLVAANSTTAAVNPLVCDYIKLVPSL"
     gene            1777..4956
                     /locus_tag="PLU11_09395"
     CDS             1777..4956
                     /locus_tag="PLU11_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN59377.1"
                     /translation="MYSTITSGFRKALGLILFLGAIQGVTAQEPAAKKDTVAVIKIQV
                     TGTILDAATGKGIVGARISLPNVSASITDASGSFKINVPTYFEDLLVTAEGYDGKQIP
                     LKGRKQISVSLLEEGYSGFQDPLTSPLSIEPMRNTTSASSRIQLSNEWTKPNETLDAL
                     LQGQVAGLNSTRRSGTPGVGANLFLRGYNSLYGTNKPLVIVDGMIYDVNDYGQSIIAN
                     NYTNPFALINVQDIDNVTVLKDAASIYGTKGTNGAIIITTSRAKQQATHIDFGAYTTY
                     NQQPSSLPVMNADDYRVYLSGMLQSKGLSSSEINAMPFMIDDTTGNPDYYRYHNNTNW
                     QNKVFNNSLTNNYFLRVTGGDNIATYALSVGYSKAEGTIRSTDMNRYNTRFNAAFNFS
                     KRFTGSANLSFTFNEQNLRDQGISDKTAPLFLALTKAPFMLDREVNDKGVTSPNFADT
                     DILGKSNPAVLINNMQAFNKYYRFSGSFKFNYAISKTLNASTLIGVTYDKVRENIFIP
                     RKGVADDTLSNAVADSRLGSQVKRLFAFYTDTRLEYSKTIRRYHQFTSRLGLRYQHSD
                     AEQDYALGFNSATDDLVSVQNGLNALRQIGGGIGEWNWMNTYFNAEYGFKNKLFLAFN
                     AAADGSSRFGTQVKNGVLINSNHFAVLPSASLAWLLSSENFMADSKIDLFKLRLSWSM
                     TGNDDIGNYSTRQSYTSQNLLGMQGLVRNGIPNPALQWETGEKFNAGVDVSALNDRLT
                     FTMDFFHSKTRNMLVYENITGAAGFNTILTNNGSMQNTGLEATVGVRLINKKNLKWDI
                     SGNIATIRNMVKHVPGGRFFTEYAGATLLTQGDRPAGLFYGYVSNGVYSTSTEAAEAG
                     LKVKNADGSYTAFGAGDIRFSDINGDKLIDENDRRVLGSPMPEYFGGFSNKLTYRRFQ
                     LEALFTFAGGHEVFNYMRYRLESASNTDNQLNSVINRWRAEGQVTNMPKATYGDPLGN
                     SRFSNRWIEDGSYLRLRHASLSYALPVKGEFVRNATIYVTGTNLLTFTKYMGYDPEFS
                     ASPSLYGQGIDTGLDPLYRSATLGVKLGF"
     gene            5008..>6151
                     /locus_tag="PLU11_09400"
     CDS             5008..>6151
                     /locus_tag="PLU11_09400"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN59378.1"
                     /translation="MKRINFLGLTLLMAIIVLPGCKKVFDIKPQDQLDVTNAYQNVYD
                     ADAAVVGIYGKFMTLADRYVILNELRGDLLNYTNNANESLRQISTHTVTAGNPYASPR
                     PFYELILNCNDALEHFQVMVQEKRMTEDEFNQRYSDIGALRSFLFLQMGIHFGEVPYV
                     TSSLTNVDAVKDPSNFPKLSFPVLLDSLIRFTEALPSKELYPSGTNLNISVDGYPTQK
                     FYINKKVILGDLHLWKGNFVKAAEWYRQVMETGTQGTVNGNYYTQYKLGWDSNGDIDH
                     YVTYSRAGDASTLVYNSQWRIMFEQAFGTAGFDREWVWALPFDSKFKPDNSLIKLFSP
                     IGGDYLVKPSNEIYNLWDNETQRAAQGGSIPYDARKLLSTSTIGGQP"
BASE COUNT         1693 a         1496 c         1410 g         1552 t
ORIGIN      
        1 ggccgcggca ctgccgggag tgacgtgcgt gcgacacaag gcggaaggca tcccgccagg
       61 tgggtgtaat tgattttacc acgactcaac agcaaacaat ccggatcacg ccgctggtag
      121 gtacgcagaa taacaataac ctggatatga ttcatttcat cccggtagat gaagaacagg
      181 tgctgccccg gttcaaaccc gatggcacca agttatattt ataaaaaagg ggctgatcct
      241 gaaaatgggt tagcagctag taatattttg aaacgactat gcccaaaggc atactaaata
      301 acagaaacat gaacagcagg aaatggatga tcgcctgtgc actgatgacg gccggatttt
      361 tcggaagcgg atgtaataag accattgatg accagaccgc tattacggat ccctcattga
      421 ataaggacct gatggaactg atcagtgaaa aagccgattt gagcaccttt gccggctacc
      481 tgaaacaaac agggtatgat gccgtgatcg cctcgtcaag aacgtatacc gtattcgctc
      541 ccggtaatgc cgaactggct accctggatg cggcggtgaa aaatgatgcg gcaaaactga
      601 aacagtttat cggcaatcat atcactaccc aggtttacag tacgaaagac gtcaccagta
      661 ctacgcggat tctgatggcc aatggcaagt atctgtctat gaatggtacg gctatcggcg
      721 cctccctcat caccactaaa gatcagtatg ccgccaatgg cctgctgcaa caagtggata
      781 aaatgcttcc ggctctcgaa aacacctggg attttgtggc tggcagtcag tctcccgcca
      841 atcaaaaagc atttatgatg tccttgttca ggaatgtctt tgatgccacc aatgctattg
      901 tgattggtat taatcagacg actggtgatc cgatatacca ggccggcacc gattctattt
      961 ttacgaatct cttctggaac aaagttgctg acctgcgcga tgagaaaaag caattcaccc
     1021 tgtttatgat gcaggatgcc gcctgggatg cggatcaggc ccgtttcgcc ccttattatg
     1081 tgaccaatac ggctgacagc aataagctgg taaccggatg gaatgttctt cgagatttag
     1141 ccgtggatac tttatatgaa cccggcagca tacccgatac gattacatca gcattcggca
     1201 cccgcgttcc cattgaacgt tctgccattg tgaatacaat ccgtaccagt aacgggattg
     1261 tctatatcat gagccggatc aatgtgcagc cttcctctaa actcaaaacc attgttattg
     1321 aaggggagaa ttacaacagc accagtcatg accgccgcgg taatacctat ttcagggaac
     1381 gctataatcc ggtaaccgga caagatttca gggatgtgga agtgtttaat cacggtgttg
     1441 ccttattcaa tatccgttac caggtggagg aaattccttc cattaagtat aaagcttatt
     1501 gggtggcggt aaatgatttt cagactgctg ctttcaacca acgcctggcc cccgggatcg
     1561 ctaccgctac cactttccct tatgtagccg tacccgtcag caatttcaac gaagtctacc
     1621 tgggtgaaat caccctggct caatatcaac cggtttacaa tttttacctg gtggcggcca
     1681 attccaccac ggcagccgtg aacccgctgg tatgtgatta tatcaaactg gtgcctagtt
     1741 tataaaactg attgattcaa gaaaacttac atagacatgt attcaactat tacaagcgga
     1801 ttcaggaaag ccttaggtct gatactcttc ctgggagcaa tacagggcgt aaccgcacaa
     1861 gaacccgccg ctaaaaaaga tacagtagcg gtcataaaaa tacaggtgac cggtaccatt
     1921 ttagacgcgg ccaccggtaa agggattgta ggcgccagga tcagtctgcc caatgtttcc
     1981 gcatccatca cagatgccag cggctcgttt aaaatcaacg tgcccactta ttttgaagac
     2041 ctgttggtaa ccgctgaagg ttatgatgga aaacaaattc ccttgaaagg cagaaaacag
     2101 atttctgtgt cgctgctgga agaaggatac agtggcttcc aggatcccct gaccagtccc
     2161 ctgtcgattg aaccaatgcg taataccacc tccgcctctt cgcggatcca gctcagtaat
     2221 gaatggacca aacccaatga aacactggat gctttattgc agggacaagt ggccggactg
     2281 aacagtaccc gccgctccgg tacacccggt gtgggcgcta atctttttct ccgcggatat
     2341 aattctttat atggcaccaa caaacccctg gtgattgtgg atggaatgat ctatgatgtg
     2401 aatgattacg gacaaagcat cattgccaac aattacacga atccttttgc gctcatcaat
     2461 gtacaggata tcgataatgt gaccgtatta aaagatgcgg cttccatcta tggtacaaaa
     2521 ggaacaaacg gggccatcat catcaccact tcccgcgcca aacaacaggc tacccatatt
     2581 gatttcggag cctatacaac ctacaatcag cagccatcct ccttacctgt gatgaatgca
     2641 gatgattacc gggtttacct ctccggtatg ttacaaagca agggtctgtc ttcgtctgag
     2701 atcaatgcca tgccttttat gatcgatgat accaccggta atccggacta ttaccgctat
     2761 cataataata ccaactggca gaataaagta tttaataaca gcctgaccaa taactatttt
     2821 ctgcgggtaa ccggggggga taatattgcc acctacgccc tgagtgtggg ttattctaaa
     2881 gccgaaggaa ccattcgctc cacggatatg aaccggtaca atacccgctt caacgccgca
     2941 ttcaatttct ctaaacgttt tacgggttcg gctaacctgt ccttcacttt caacgaacaa
     3001 aacctgagag atcagggtat ttctgataaa acggccccct tattcctggc actgaccaaa
     3061 gcccccttta tgctggaccg ggaagtcaat gataaaggag tcacttcccc caactttgcc
     3121 gataccgata tcctgggtaa gagtaacccg gccgtcctga tcaataacat gcaggcattt
     3181 aataagtact accggttctc tggttctttt aaattcaact acgctatttc aaaaacactg
     3241 aatgccagca ccctcatcgg ggttacatat gataaggtaa gagagaatat ttttattccg
     3301 cgtaaagggg tagccgatga tacgctcagc aatgccgtgg ccgacagccg gctgggttca
     3361 caggtgaaac gcctcttcgc tttttatacg gatacaaggc tggaatacag caaaaccatc
     3421 cgccgttatc accagtttac ttcccgcctc ggattacgtt accagcacag tgacgccgaa
     3481 caggattatg cactcggatt caattcagcc acggatgatc tcgtaagcgt tcagaacggt
     3541 cttaacgccc ttcgccagat aggcggcggt atcggtgagt ggaactggat gaatacctat
     3601 ttcaacgcag agtacggatt taagaataaa ctcttcctgg ctttcaatgc agcagctgac
     3661 ggatcctccc gtttcggtac acaagtgaaa aacggggtac tgatcaacag caatcatttc
     3721 gcagtactgc cttctgccag cctggcctgg ttactgtctt ccgaaaactt tatggccgat
     3781 tctaagattg acctgtttaa actccgcctt tcctggagca tgaccggcaa tgatgatatt
     3841 ggaaattact ccacccgcca gtcctatacc tcccagaacc tgctgggtat gcagggactg
     3901 gtacgtaacg gtattcccaa tccggcttta caatgggaaa ccggtgagaa gttcaatgca
     3961 ggggtggatg tatcagcttt aaatgaccgg ctgactttca ccatggactt cttccattcc
     4021 aagacccgca acatgctggt ttacgaaaac attaccggag ctgccgggtt caataccatc
     4081 ctgacgaata atggaagtat gcagaatacc gggctggaag ctacggtggg tgtacggctg
     4141 atcaataaaa agaacctgaa atgggatatc agtggaaaca ttgccaccat acggaatatg
     4201 gtgaaacatg ttccgggcgg ccgtttcttt accgaatatg caggagctac cttacttaca
     4261 cagggtgatc gtcctgccgg tttattctac ggctatgtaa gtaatggcgt ttacagtact
     4321 tccaccgaag cagctgaagc cggattgaaa gtgaaaaatg cggacggaag ttataccgca
     4381 tttggagccg gtgatattcg tttctccgat atcaatgggg ataaactgat tgatgaaaat
     4441 gaccgccgtg tactgggcag cccgatgccg gaatactttg gtggattctc caacaaactg
     4501 acttatcgcc gctttcagct ggaagcactc ttcacctttg ccggcggaca tgaagtgttt
     4561 aactatatgc gctaccgcct ggagtctgcc agtaataccg ataatcaact caacagtgta
     4621 atcaatcgct ggagagctga aggccaggta accaatatgc cgaaagccac atacggagac
     4681 ccgctgggca atagtcgttt cagcaatcgc tggatagaag atggttctta cctccgtctc
     4741 cgtcacgctt ccctgtctta tgcgttacct gtgaaaggag agtttgtccg gaatgcaact
     4801 atatatgtaa ccggaaccaa cctgctgaca ttcacaaaat atatgggata tgatccggaa
     4861 ttcagtgcca gccccagcct ctatggacag ggaattgaca ccggactgga tccgctgtac
     4921 cgctctgcca ccctgggtgt taaactcggg ttctaagctc attaattgaa aagaaacaga
     4981 tttgctgaca tgaataataa aataactatg aagcgtatca attttctggg actaaccctg
     5041 ctgatggcca tcattgtttt gcctggttgc aaaaaagtgt ttgacattaa accccaggac
     5101 cagctggatg tgaccaacgc ttatcagaat gtgtatgatg ccgatgcggc cgttgtgggt
     5161 atctatggaa agtttatgac actggctgac cgctatgtga tcctcaatga acttcgtggc
     5221 gacctgttga attataccaa taatgccaac gagtcactgc gccagatcag cacccatacg
     5281 gttacagccg gtaatcccta tgccagcccc cgtccgtttt atgaactgat ccttaactgt
     5341 aacgatgcgc tggaacattt ccaggtgatg gtgcaggaaa aaaggatgac ggaagatgag
     5401 tttaaccagc gctattccga tatcggggcc ttacgttcct tcctgttcct gcaaatgggt
     5461 attcattttg gtgaagtgcc ttatgtgacc agttccttaa ccaatgtgga tgcagtaaaa
     5521 gacccttcta atttccctaa gctcagcttc ccggtgttgc ttgacagcct gatccgtttt
     5581 acggaagctc tgccctctaa agaactctat ccttccggta ccaacctgaa tatttcagtg
     5641 gatggatacc ccacacagaa attttatatc aataagaaag tcatcctggg tgatctccat
     5701 ctctggaaag gtaattttgt gaaagccgca gaatggtacc gccaggtaat ggagacaggt
     5761 acacaaggaa cggtgaatgg taactattat acacagtata aactgggttg ggatagtaac
     5821 ggcgatattg accactatgt tacctactca cgcgccggtg atgccagcac attggtttat
     5881 aattctcaat ggcgtatcat gtttgagcag gctttcggaa ccgccgggtt tgaccgcgaa
     5941 tgggtttggg ccttgccttt tgatagcaaa ttcaaaccgg ataattcact gatcaaactc
     6001 ttttcaccga tcggaggaga ttaccttgta aaaccatcca atgagattta taatctttgg
     6061 gataacgaaa cacaacgggc tgcacagggt ggttctattc cttacgatgc ccgtaaatta
     6121 ttaagtacct caaccatcgg cggacaaccc g
//
LOCUS       DAOPMW010000184         6142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_14723_length_6142_cov_8.118613, whole genome shotgun sequence.
ACCESSION   DAOPMW010000184 DAOPMW010000000
VERSION     DAOPMW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14723_length_6142_cov_8.118613"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="PLU11_09405"
     CDS             <1..1824
                     /locus_tag="PLU11_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HPN59379.1"
                     /translation="EQIQAINPHPMGLWYDNARKSWLLYNEDLALFPKDIHLPGVLRN
                     YTGHTVGVDHFVAFLQVKLTAAQIQDYYAVIDDPALNNHPDLRIQVTHLYNNDGTTTG
                     MYNADYPMAFYDRERNKWILRNKNKTPLKAGLVFNFAATAAEIKTLPKQEGLAIKKNA
                     GALLRQEKIKFRVTINGFTCHTPTVDHMLEVDGKGDEVFFGGITQYYKLADSSKVGPV
                     QRNWSVRYGDINSEEGRMGLRIKAGSKPGNLGGVQTGDQFPALEPWKRKAPVYTNTLP
                     LLLTEGELLKGVNSALVVPALWEFDGTTEEEQALNQFAEHITKAAVYFTATGVLMVTI
                     GPIALPFMTVSTMMVQMNSRGDMMNNQPAANLPVFSYLDFRIPEFNVFVAKTMYGDAK
                     DRPVGMTEVKEGYEYKPLGMLLNYESAIALSQSDFGYGKGIIPIRYKDAPDMKGDYTV
                     YIQVETTDEKGSFDKTAIVSPVSKANPKPYRLQNVYSGMNLMNNATGPVANQAQVANK
                     WQFELADRFNDVYFIKNLDGRYLYITPPSGVQETNGFPVKSGPKPTGNAGKWKVVSNS
                     DGTYSIINMYTKKALIHDGPSGRILIWENRANPEQRWMIGR"
     gene            2141..2815
                     /locus_tag="PLU11_09410"
     CDS             2141..2815
                     /locus_tag="PLU11_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN59380.1"
                     /translation="MKKVVLFISGFICFLNISAQHQEEHNKNKTAQSANEHMHQSSVD
                     ELSRRFESPERDAYQQPEKVLEYLGNIKGKKIMDIGAGTGYFSVKLAAKGAQVIAADV
                     SDEFQQYLKARIEKNQLKNIELRKIPYDHPGLNEKEADMILIVNTYHHIENRSVYFTH
                     VKKGTAAGGELVIIDFFKTDVPVGPPPAHKVSMDEVIAELKAAGYTSFEVNVNLLSYQ
                     FIIRAR"
     gene            2884..4389
                     /locus_tag="PLU11_09415"
     CDS             2884..4389
                     /locus_tag="PLU11_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPN59381.1"
                     /translation="MKAVTLLLSLALTLLFVGCKKKTTPDPVTESDQSSFAAATVINQ
                     RLGRGINLGDTYEAAWAPREADPADFQRIAASGFKHVRIPVRWEIPTRSSYSAPYIIQ
                     PAFLQSVKKAVDAALKNKLHVIINMHHHDSLFKNPDGLKPMFIAQWKQIASYFKQYSD
                     SLLFEVLNEPHDVLDADKWNLFFADALTEIRKTNPKRTVLLGVAEWGGVSALNKLVTP
                     SDTHLILTIHYYSPFSFTHQGAEWVSGSNAWLGTKWNDTEYDREAVVNDFQTVLRIKQ
                     EKNIPIHIGEFGAYSKADMNSRVKWSRFLSRWFEQNGFSWAYWEFNAGFGIYDPATGQ
                     YRTALVNALTTEAMTRPTPVNLIDLYSSNFGTSQDGWNLYNNDASASSSVSIVSGKVQ
                     LAITSPGNQNWHIQLIRPGIAIQSGKTYRVSFNAYSTGSRSLAASLMLNNSPWTTYSY
                     SDFTLSATDKSYSFVFTAGATDPGAAFVFSMGNGGVLPVTIYNIKFQEIQL"
     gene            4468..5190
                     /locus_tag="PLU11_09420"
     CDS             4468..5190
                     /locus_tag="PLU11_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59382.1"
                     /translation="MNLLSTILNIFKTRKPSVEDEPHEIRYKRMYNDPGIFQYEENGF
                     TVQLENKKVQVNWSELERLVAYKADMMTIDEIRMEISYRNKSIIITEDTPGWYQFVLK
                     TKNIFASIPEDWDINIIQPAFETNLTLLYEREDRVMPEKNNFYAAFTGTTKKRVISAF
                     DQNEWSCREIRTSEYELNTSWAELILHGKEDAPLLNGIVAYHPDNIAILDRILKAVGG
                     LDAYEFYDADNNLLFETKNGAH"
     gene            5212..5640
                     /locus_tag="PLU11_09425"
     CDS             5212..5640
                     /locus_tag="PLU11_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59383.1"
                     /translation="MYAEEHDKIIEQLRRYIREDDATFGIFQYGGGADESYIKADKQG
                     LRLFALELLMAAQKSNEILHDKEKSIIPIDFEKSWVNEDSDTFVQYVMPVVSRSNARQ
                     PVGPTNTIKDKLIGAGFIALMVFLILSMIVGVFSIIKWLL"
     gene            5688..>6142
                     /locus_tag="PLU11_09430"
     CDS             5688..>6142
                     /locus_tag="PLU11_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPN59384.1"
                     /translation="MMSDASFRKIAMDFAEVTEEPHFEKTSFRIKGKIFASMDTVNKR
                     VTLKLPPNEQAIYLLMKPAFASPATGAWGKQGWTRFELTKVPAPVMREALKKAYETVA
                     PIKKSLKPSKKIVIRKEKAEMIQTLHPDKTKTNKRISLEKYNFIKANLLA"
BASE COUNT         1825 a         1233 c         1482 g         1602 t
ORIGIN      
        1 gaacaaatac aagcaatcaa tccgcacccg atgggactgt ggtacgataa cgcacggaag
       61 agctggttgc tttacaatga ggatttggcg cttttcccaa aggatattca cttgcccggc
      121 gtattgagga attataccgg tcatactgtc ggtgttgatc attttgttgc atttctccag
      181 gtaaaactga cggcagccca gatacaggat tattatgcgg tgattgatga cccagcgctt
      241 aacaatcatc cggatttgcg gatacaggtt acccatttat ataataatga tggaaccacc
      301 acagggatgt ataatgccga ttacccgatg gctttctatg acagggaacg gaataaatgg
      361 atcttgcgta ataagaataa gacgccactg aaagcgggac tggtgttcaa ttttgccgcc
      421 acagccgctg aaatcaaaac actgccgaag caggagggcc ttgctattaa gaagaatgca
      481 ggggcattac taagacagga gaaaattaaa ttcagggtaa cgatcaatgg attcacctgt
      541 catacaccca cggtggatca tatgctggaa gtggatggaa agggagatga agtattcttt
      601 ggcggcatca ctcaatatta taaactggcc gacagcagta aggttggacc ggtacaacgc
      661 aactggtcgg tccgctacgg ggatatcaat agtgaagaag gaagaatggg attaaggata
      721 aaagccggat ccaaaccggg caacctgggt ggcgtacaga cgggagatca gttcccggca
      781 ttggaaccct ggaaaagaaa agcacctgtc tataccaata cgcttccctt attgctaact
      841 gaaggggagt tactcaaagg ggtgaattca gcattggtgg tgccagccct ctgggaattt
      901 gatggtacga cggaagaaga gcaggcattg aatcagtttg ccgaacatat caccaaggct
      961 gcggtttatt tcaccgctac aggagtgttg atggtaacaa taggccccat tgcccttcct
     1021 tttatgactg taagtaccat gatggtacaa atgaatagca gaggagatat gatgaacaat
     1081 cagccggcag ccaacctgcc tgtgttttcg tacctggatt tcaggatacc ggaattcaat
     1141 gtgtttgtgg ccaagacaat gtatggtgat gctaaagacc ggcctgtggg tatgacagaa
     1201 gtgaaagaag gatatgagta taagcccttg ggtatgttgt tgaattatga atctgccatt
     1261 gccctctccc aatcggattt cgggtacggt aaaggcatta tccctatccg gtataaagat
     1321 gctccggata tgaaagggga ttatacggtt tatattcagg tggagacgac agacgaaaag
     1381 ggcagttttg ataaaacagc aattgtttca cctgtttcaa aagcaaatcc gaagccttac
     1441 cggttgcaga atgtttactc cgggatgaac ctgatgaata atgcaaccgg tccggtggcc
     1501 aaccaggcac aggtggccaa taagtggcaa tttgaactgg cagatcgctt caatgatgtg
     1561 tattttataa agaatctgga tggaagatat ttatatatca cgccaccttc gggtgtgcag
     1621 gagaccaatg gctttccggt aaagtccgga ccaaaaccaa caggtaatgc cggtaaatgg
     1681 aaagtcgttt ctaatagtga tggcacctat tctatcatta acatgtatac gaaaaaggcc
     1741 ttgatccatg acggtccttc cggacgtatc ctgatctggg agaaccgggc taatcccgag
     1801 caacggtgga tgatcgggag atagggagag gagggagtcg ggaggaggga gtcaggagtc
     1861 gggaggagtg agtcgggaga tagggagtca ggatagagca acccgtaaag tttctgtaat
     1921 ttagaaatac agaaaccacc gatcacctaa tctgcacgta tgcgcttatc ctttctcttt
     1981 tttatcacta ctgctcaacc ggtttaagag tttagaatgc cgacccggtg ggagaagcag
     2041 gaagcggtac attttacaat tacattcaat acagggtttt tgataaggct ggtaaaacca
     2101 gctgataagc cttaccttta tatccatcag cattttgtat atgaaaaagg ttgttttatt
     2161 tatttctggt tttatttgct ttctgaatat ctctgcccag caccaggaag agcataacaa
     2221 gaataaaacc gcacaatcgg ccaatgagca catgcatcaa tcctccgtcg atgagttgtc
     2281 aaggcgcttt gagtcacccg agcgggatgc ctatcagcag ccggaaaaag tactggaata
     2341 tctgggaaat attaaaggaa aaaagatcat ggatatcgga gctggtaccg ggtatttttc
     2401 agttaagctg gcagcaaaag gtgcacaagt gattgctgcg gatgtcagtg acgagtttca
     2461 gcaatacctg aaagcaagaa ttgaaaagaa tcagctcaaa aatatagagt tgcggaaaat
     2521 accctacgat catcccgggt taaacgaaaa ggaagcggat atgatcctga ttgtaaatac
     2581 atatcatcat atcgaaaacc ggtccgtcta ttttacccat gttaaaaaag gcacagctgc
     2641 tggcggtgaa ttagtcatca tcgatttttt taaaacagat gtacccgttg gtcctccacc
     2701 ggcccataaa gtatctatgg atgaagtgat agccgagttg aaagcagctg gctataccag
     2761 ctttgaggtg aatgttaatt tattgtctta tcagttcata ataagagccc ggtaatacaa
     2821 gagtttcacg tttctgtgtg cttctgattc gtaattccat agaatataga ctaactttat
     2881 tctatgaaag cggtaacctt actcctttcc ctggctctca ccttattatt cgtcggttgc
     2941 aaaaagaaaa caactcccga tcctgtcact gagtccgatc agtcttcttt tgctgctgct
     3001 acggtcatta atcaacggct cggccggggt ataaatcttg gtgataccta tgaagccgca
     3061 tgggcgcctc gggaagctga tccggccgat tttcaacgga tcgcagccag tggtttcaaa
     3121 catgtccgca ttccggtacg ttgggaaata cccacccgca gtagctactc ggctccgtat
     3181 atcattcaac ctgctttttt acagtcggta aagaaagccg tagatgcggc cctgaaaaat
     3241 aaacttcatg tgatcatcaa catgcatcac catgatagtt tgtttaaaaa ccctgatgga
     3301 ttaaagccga tgttcatcgc ccaatggaaa cagatcgctt cctattttaa acaatactcc
     3361 gacagtcttt tatttgaggt actcaatgaa ccgcatgatg tgctggatgc ggataaatgg
     3421 aacctgttct ttgccgatgc gctgacggag atccggaaaa ccaatccgaa aagaaccgta
     3481 ttgctgggcg tggccgaatg gggtggggta tcggccctga ataaactcgt gactccgtcc
     3541 gatacgcatt tgatcctgac aattcattat tacagtcctt tttcatttac gcaccagggg
     3601 gcagaatggg tcagtgggtc gaatgcctgg ctgggcacta aatggaatga tacagagtat
     3661 gaccgggaag cggtggtgaa tgatttccag accgtactca ggataaagca ggaaaaaaat
     3721 atacccatac atatcgggga gtttggtgcg tacagtaagg ccgatatgaa ttcgcgggta
     3781 aaatggtccc gtttcttatc gcgttggttt gagcagaacg ggttcagctg ggcctattgg
     3841 gaattcaatg ccggtttcgg gatatacgat ccggcaacag gtcagtatcg taccgccttg
     3901 gtgaacgcac ttactacaga ggcaatgacc aggcccaccc cggtgaacct gatcgatctt
     3961 tacagcagta atttcggtac ttcgcaggat gggtggaacc tttataataa tgatgcttct
     4021 gcttcttctt ctgtaagtat agtttcagga aaggtacagt tggccataac cagtccgggt
     4081 aatcaaaact ggcatatcca gttgatcaga cccggcattg ctatacaatc gggcaaaacc
     4141 taccgcgtga gttttaatgc ttattctact ggcagccgca gcctggctgc ttcccttatg
     4201 ctaaacaatt ccccctggac cacttatagt tattcggatt ttaccctttc tgcgacagac
     4261 aagtcttata gctttgtgtt caccgccggc gctacagatc ccggggccgc ttttgttttt
     4321 tcaatgggga atggaggggt gttaccggtg acgatttata atattaaatt tcaggagatt
     4381 cagctttaat cagaaagttg aacaataaaa attaactgac tttgccgtac aacttttttc
     4441 attggataat gctgttaaat ttaagcattg aacctgctta gcaccatact taatattttc
     4501 aaaaccagga aaccgtctgt ggaggatgag ccgcatgaaa tccggtataa gcggatgtat
     4561 aatgacccgg gtatttttca atacgaagag aatggattta cagttcagct ggaaaataaa
     4621 aaagtacagg tgaactggtc agaattggag cggttggttg cttataaggc agacatgatg
     4681 actatcgacg aaataagaat ggagatcagt tatagaaata aatcaataat tattacagaa
     4741 gataccccgg gatggtatca gtttgtttta aagacaaaaa acatattcgc cagtattcct
     4801 gaagactggg atatcaatat tattcaaccg gcttttgaga caaatctaac cttattgtat
     4861 gagcgggaag accgggtgat gccagagaag aataacttct atgcagcatt tacaggtaca
     4921 acaaaaaaaa gagtcatttc tgcttttgat cagaatgaat ggagttgccg cgaaatacgg
     4981 acttccgagt atgagttaaa tacgagttgg gcagaactta ttcttcatgg gaaagaagat
     5041 gcgcctcttt tgaatggaat tgttgcatat catccggata atatagccat actagaccgg
     5101 attttgaaag ctgtgggagg gttggatgcg tatgagtttt atgatgcgga caataatctt
     5161 ttatttgaaa caaaaaatgg tgcccattaa tcgactaaaa caacttcaac aatgtatgct
     5221 gaagaacacg ataaaatcat cgaacaactg aggcgctaca tccgggagga tgatgccaca
     5281 ttcggtattt tccagtatgg cggcggtgcc gatgagtctt atattaaagc agataagcaa
     5341 ggactccggc tttttgccct cgaactcttg atggctgcac aaaaatcgaa tgaaattctg
     5401 catgataagg agaagagtat cattccgatt gattttgaaa aaagctgggt gaatgaagat
     5461 agtgatacgt ttgttcaata tgtgatgccc gttgtgtcac ggtcaaatgc cagacagccc
     5521 gttggaccaa cgaataccat taaagacaaa ctgattgggg ccgggtttat agctttaatg
     5581 gtttttctca ttctttcaat gattgtcgga gtattttcaa taatcaaatg gctgctatag
     5641 agatattaca gcatacggct aattcataat ttttaaacct gcaaaatatg atgtcagacg
     5701 cctcattccg caagatcgct atggactttg cggaggtcac agaggaaccc cattttgaaa
     5761 aaacttcttt tcgcattaag ggcaaaatat ttgccagcat ggatacggtt aataagcggg
     5821 tgacactaaa actgccgccc aatgaacaag cgatttattt gttgatgaaa ccggcatttg
     5881 ccagtccggc cacaggtgcc tggggcaaac agggatggac ccgttttgaa ctgacgaaag
     5941 tcccggcacc agtgatgcgt gaggcattga agaaggcata cgaaacagta gctccgataa
     6001 agaaatcgct gaaacccagt aagaaaatag tcatccggaa agagaaggcg gaaatgattc
     6061 agaccctgca cccggataaa acgaagacca ataagcgcat ttcgctggag aaatacaatt
     6121 ttataaaagc aaatctgctg gc
//
LOCUS       DAOPMW010000185         6044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15052_length_6044_cov_8.000167, whole genome shotgun sequence.
ACCESSION   DAOPMW010000185 DAOPMW010000000
VERSION     DAOPMW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15052_length_6044_cov_8.000167"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1554)
                     /locus_tag="PLU11_09435"
     CDS             complement(64..1554)
                     /locus_tag="PLU11_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59385.1"
                     /translation="MMKRLHNIAQHYFWPILLLPAFFIIHVATENPELVRPAPSIKLW
                     SIYVLVGVLMAFCFRLYFRNWPKAILMSFCVLCFQFFFGAVYDSLQTHFHGYFFSRYR
                     FILSVVTIVLLFIAWRLKKSGSVFGGLFLYLNLLFVLLISADLITLLSVKRPNNRAPI
                     ESGNLLPVTGKKPDIYLIVADGYPGKAALQEYFRYNNDRFTDSLRRSGFFVNENAVSN
                     YNHTTHSMASMLNLTYLSDIRNNVVDKKEVNRGLDLINQNIFTRYLIQNGYGIINHSI
                     FTIHEIPAALNPGFLPTSSTIITNQTFTGRFLKDLVFQLAHIYHFKKLVRYFHSPVME
                     GNQALPAKTVIKAAEKDQPPRLVYTHLIMPHYPYLKDSSGNETDEFRWKEVRDSSRFI
                     SYLKHCNQQLLSLVSHIRQASARPPVILLIGDHGFREFNKPRFNPLQFSCLQAVYYPD
                     GNYQSFYNGMSQVNLFRVLLNDQFGQQLPLLKDSSITLTDKTPFPH"
     gene            complement(1551..3026)
                     /locus_tag="PLU11_09440"
     CDS             complement(1551..3026)
                     /locus_tag="PLU11_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59386.1"
                     /translation="MKQLLRQKPVFLLLLPAFFVLHGFRENYGVVPVPIAWLLFGTYV
                     AVSLVLTGLAWIGYRQLMKAALLAACMMIFHFFFGAVHDLLRNWFPGSLITRHTFILP
                     FSLFLFIALAIFIKKRKSGFTGTAYFFNVLLLLLLALDFLWLSFTLAGQAKHTVAEEK
                     LNWINCDTCKKPDIYLVIADGYPGKKELTDILKYDNQRFEDSLRQLGFFITDSSMSNY
                     NFTTYSMASMLNMEYLQPIIGRYRNKTDLAYCYERIRNSKTVNQLRKMDYEIINNSIF
                     DIEDLPSSAIPTFLPRKSEPITAQTFLSRMEKELAYHLATTLKIDFVVNFLQNHDLKN
                     NRELMRKTKELFPKQTQKPRFVYTHLVMPHRPYYFDSTGAPLPLDTLTEEFGKNVPAF
                     ISYLKYCNRELTGLVRYIKENSPTPPVILLMSDHGFREFSGPVDNIYQFYTLNAIYLP
                     GGNYAPFYKGMSNVNQFRVLFNTSFGQQMSMLKDSTSYLID"
     gene            complement(3035..4411)
                     /locus_tag="PLU11_09445"
     CDS             complement(3035..4411)
                     /locus_tag="PLU11_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59387.1"
                     /translation="MNEKPDALPSSFRDPAGYVFRQENIYYRLITEAGKISYDGLMNS
                     GLYATLTQKKILLPHTEMPASFQGPAGYKIIQPEQLPFITYHWEWPFALLQDAALLTL
                     ETAKLALDHGMVLKDATPYNIQWHNGDLAFIDTLSFEPYKEGQPWIAYRQFCESFLSP
                     LLLMHYRKHSLHSLQLGWPDGIPLQITRSLLPWKSRFSVLTWMHIHLHSRYAAKPGGQ
                     KDKTAVVSLAKFRQLLSSLEMLIRSLKLEDEGTTWGDYYTEAATRNDYLDQKEKIIES
                     WTRELTGINNAVDFGANNGSFSQPLAAKGIFTVAADGDPVAINKLYKKIRAGNLKNLL
                     PLIVDFTNPSPAMGLGNEERASFLQRIGTNNLGLCLAFIHHLCIGKNIPFERVAALMA
                     LSCKQLIIEFVPKEDEKVQALLILKKDIYTDYTIDAFEAAFHPYFNIQEIKKIPGSAR
                     TLYLMIRK"
     gene            complement(4404..4562)
                     /locus_tag="PLU11_09450"
     CDS             complement(4404..4562)
                     /locus_tag="PLU11_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59388.1"
                     /translation="MIQDLIYIDPGSGSYLVQVIIGALLGVVMFFKNIKMYLLSFFKR
                     SPKKQKDE"
     gene            complement(4559..5665)
                     /locus_tag="PLU11_09455"
     CDS             complement(4559..5665)
                     /locus_tag="PLU11_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN59389.1"
                     /translation="MAKAAKAKKSIITDQSYSFLKSYINNASPVGFETWGQKLWIEYL
                     KPYVDSHYVDPYGTAVGVINADHPFKIVIEAHADEISWFVNYITADGLIYLKRNGGVD
                     HQTAPASRVLIHGKKGPVKAVFGWPAIHTRLQPDQKETSPRTDNLWLDCGARNKKEVA
                     ALGIHIGAVVTYQDGFDELANGNLIGRAFDNRVGGFMIAEVARLLKENKKKLPFGLYV
                     VNAVQEEIGLRGAEMIARRIKPNIAIVTDVTHDTTTPMINKNIEGDVSTGKGPSLAYA
                     PAVHNKLLAHVEKVAGDKKIPVQWRALSRSTGTDTDAFAYSNDGCPSVLISIPLRYMH
                     TTVEMLHRNDIENTIKLMYETLLSLSPKTNLNYL"
     gene            complement(5696..>6044)
                     /locus_tag="PLU11_09460"
     CDS             complement(5696..>6044)
                     /locus_tag="PLU11_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59390.1"
                     /translation="FTISRYFAVYMICILFDYRDRTDDKNAGIRSLITYMEPTGIRRL
                     FLFSFLIFILSTLGLYGQRLSAESVLLILIPGFIVEGLYRRATRDFSDMLYYFVLDGL
                     MAFSALLTLFTRI"
BASE COUNT         1644 a         1310 c         1476 g         1614 t
ORIGIN      
        1 tcagttcgga caacagcttc cgttactgaa agacagcagt atcaccctgg ccggtcggat
       61 gaatcaatgg gggaagggtg ttttatcggt cagggtgata ctgctgtctt tcagtaacgg
      121 aagctgttgt ccgaactgat cattcagcag cacccggaat aggttcacct ggctcatacc
      181 gttataaaag gattggtaat taccatccgg ataatacacg gcctgcagac aggagaactg
      241 gaggggatta aatcggggtt tattaaactc ccggaaacca tgatcgccaa tcagcaggat
      301 gacgggtggt cgggcagatg cctggcggat atggctgact aacgatagca actgttgatt
      361 gcaatgttta agataactaa tgaaccggga actgtcccgc acctctttcc acctgaattc
      421 atcagtctcg tttccgctgc tatccttcag gtatgggtaa tgcggcatga tcagatgggt
      481 gtacaccaac cgtggcggct ggtctttttc cgctgccttt atcaccgttt ttgcgggcag
      541 tgcctggttg ccttccataa ccggcgaatg gaaataacgg acaagttttt taaagtgata
      601 aatatgggcc agctggaata cgaggtcttt tagaaagcgg ccggtaaatg tctgattggt
      661 gataatagtg ctgctggttg gcagaaatcc cggatttaat gcagcaggaa tttcatgaat
      721 ggtgaagatg gaatgattga tgatcccata tccgttttgt attaggtacc ttgtaaagat
      781 attttgattg atcaggtcta gtcccctgtt tacttctttc ttgtccacca cattatttct
      841 gatatcactg aggtacgtca ggttcagcat agaagccata gagtgggtgg tatgattgta
      901 attgctgacc gcattttcat ttacgaaaaa tccactgcgt cgtaaactgt cggtaaaccg
      961 gtcattatta taccggaagt attcctgtaa agccgcttta cccggataac cgtctgccac
     1021 tatcaaatag atatccggtt tttttccggt aaccggcagg agattcccgg actcaatagg
     1081 agccctgtta ttgggccgtt ttacagacag cagagttatc aggtctgcag aaatcagcag
     1141 gacaaacagg agattgaggt acaggaaaag cccgccgaat acactgcctg attttttcag
     1201 ccgccaggca ataaagagaa gaacgatagt caccaccgaa agtataaacc ggtaacggct
     1261 gaaaaaatag ccatgaaaat gggtttgaag gctgtcataa actgcgccaa agaaaaactg
     1321 gaaacacagt acacagaaac tcatcagtat tgcctttggc cagttgcgga aatagaggcg
     1381 gaagcaaaag gccatcaata caccaaccag cacatagata ctccatagtt ttatggaagg
     1441 ggcaggccgg accagttccg ggttttcagt ggcaacatga ataatgaaaa aagccggcaa
     1501 taagagtata ggccagaaat aatgttgggc aatattatga aggcgtttca tcaatcgatc
     1561 aggtagctgg ttgaatcttt cagcatggac atctgttgcc caaacgatgt attgaaaagc
     1621 accctgaact gatttacatt actcattcct ttatagaaag gggcatagtt gccgccagga
     1681 aggtaaatgg cattcagggt ataaaactgg tatatattat caacagggcc ggaaaattca
     1741 cgaaatccat gatcgctcat cagcagaata acaggggggg tgggcgaatt ttcttttata
     1801 taacgaacca ggccggtcag ctcccggtta cagtatttca gatagctgat aaaggccggt
     1861 acgttttttc caaactcttc tgtcagggta tccagtggca ggggcgctcc ggtactgtca
     1921 aaataatagg gacgatgcgg catgaccagg tgggtataaa caaaccgggg tttttgagtt
     1981 tgtttaggaa acagttcttt tgtttttcgc atcagctcgc ggttattttt taagtcatga
     2041 ttctgcagaa agtttactac aaaatcaatt tttaaagtgg tggcgagatg ataggcaagt
     2101 tccttttcca tcctcgacag gaaagtctgc gctgtaatag gttcagattt acggggtaaa
     2161 aaagtgggga tggcagagga gggcagatct tcaatatcaa atatgctgtt attgatgatc
     2221 tcatagtcca ttttccggag ctggttgacc gttttgctgt tccggatacg ctcatagcaa
     2281 taggccagat cggttttatt ccggtaccgg ccgatgatcg gctgaaggta ttccatgttc
     2341 agcatggagg ccatggagta agtggtgaaa ttataattgc tcatgctgct gtctgtaata
     2401 aaaaatccga gctgacgcag gctgtcttca aatcgttggt tatcatattt gagaatatcc
     2461 gtcaattcct tttttccggg ataaccgtcg gcaatcacca ggtagatatc cggctttttg
     2521 caggtatcac agtttatcca gttcagcttt tcttctgcaa cggtgtgttt tgcctggccg
     2581 gcaagggtaa aagaaagcca tagaaaatcg agtgccagca ggaggagcag cagcacatta
     2641 aaaaaatagg cggtgccggt aaacccgctt tttctctttt taataaaaat agcaagggca
     2701 ataaaaagga acagcgaaaa cggcaggata aaagtatgac gggtaattaa agaacccgga
     2761 aaccaattcc ggagcaggtc atgaacagcg ccgaagaaga aatgaaagat catcatacac
     2821 gcggccaaaa gggcggcttt catcagttgc cggtaaccta tccaggccag tccggtcagc
     2881 accagggata cagctacata ggtgccgaat aaaagccagg ctatcggaac cggaaccacc
     2941 ccataatttt cccggaatcc atgcaataca aagaatgccg gcaataatag caggaaaacc
     3001 ggtttctgcc ggagcagttg cttcattttt tctttcattt tctgatcatc aggtaaagcg
     3061 tcctggcaga acctggtatt tttttaattt cttggatgtt aaaatacggg tggaaggcgg
     3121 cttcaaaagc atcaattgta taatccgtat aaatatcttt tttcaggatc agcagggcct
     3181 gaactttttc atcttctttt gggacaaatt caataatcaa ctgcttgcag gagagggcca
     3241 ttaatgcagc tacccgttca aagggaatat tttttccgat acagagatga tggataaagg
     3301 ccaggcaaag accgaggttg ttggttccga tccgctggag aaaagaagct ctttcctcat
     3361 tgcccaatcc catggcgggt gaagggttgg tgaagtcaac gatcagcggc aacaggttct
     3421 ttaaatttcc ggcccgtatt tttttataaa gtttattaat ggcaaccgga tctccatctg
     3481 cggccacggt gaaaatacct tttgcggcca atggctggga aaaactgcca ttgttggcac
     3541 caaagtctac ggcattattt atccccgtta attctcttgt ccacgattca ataatttttt
     3601 ctttctggtc caggtaatca ttccgggtgg cggcctccgt ataataatca ccccaggtag
     3661 tcccttcatc ttccagtttc aaggaacgga tcagcatctc caggctgctg agtaattgcc
     3721 tgaatttagc gagggaaacc actgctgttt tatccttttg tccaccgggc tttgccgcat
     3781 accggctgtg caggtgaata tgcatccagg tcagcaccga gaaacggctt ttccatggaa
     3841 ggagcgagcg ggttatttgt aaagggatgc catcgggcca gcccagttga agggaatgca
     3901 gggaatgttt tcggtaatgc atcagcagga gcggggaaag aaaggattcg caaaactgcc
     3961 ggtaggcgat ccatggctgt ccttctttat aaggttcaaa tgaaagggta tcaataaaag
     4021 ccagatctcc attatgccac tgaatattat agggtgtggc atctttcaat accatgccat
     4081 gatccagggc cagctttgcc gtttccaggg tcagcagggc tgcatcctgt aacaaagcaa
     4141 atggccactc ccaatgatag gtgataaagg ggagttgttc gggttgaatg attttataac
     4201 cagccggtcc ctgaaaggaa gccggcattt ccgtatgcgg gagtaatatt tttttttgcg
     4261 tcagtgtggc atatagcccg ctgttcatca gtccgtcata ggaaattttc ccggcttcgg
     4321 tgatcagccg gtaatagata ttttcctggc ggaatacata tcccgccggg tccctgaatg
     4381 aagaaggtaa agcatcaggc ttttcattca tccttctgtt ttttgggcga acgtttaaaa
     4441 aaggaaagga ggtacatttt tatattcttg aaaaacatca ccacccccag taaggcgccg
     4501 ataatcacct gtaccaggta actgccgctt ccgggatcaa tatagatcag gtcttgtatc
     4561 acaggtaatt caggttggtt ttgggagaca gggataataa ggtttcgtac atcagtttga
     4621 tggtattttc gatatcattc cggtgcagca tttccaccgt ggtgtgcata tagcgcagag
     4681 gaatggagat caggacggag ggacaaccat cattggaata ggcaaaagca tcggtatcgg
     4741 taccagtgct gcggctgagc gcccgccatt gaaccggtat ctttttatca cccgccactt
     4801 tctccacatg ggccagcagt ttattatgca cagccggtgc ataggccagt gaaggacctt
     4861 tgccggtact tacatcccct tcaatatttt tattgatcat cggggtggtg gtatcatggg
     4921 tcacatcggt cacaatcgca atattgggct ttatccggcg ggcgatcatt tcagcgcccc
     4981 gcagtccgat ctcttcctgc acggcattta ctacatataa accaaagggg agtttctttt
     5041 tattttcttt caggagacgg gccacttcag caatcataaa tccaccgacc cggttatcaa
     5101 aggcacgtcc gattaaattg ccattggcca gttcatcaaa tccatcctgg taggtcacta
     5161 cggcaccgat atggataccc agggctgcga cttctttttt gtttcgggcg ccacaatcca
     5221 accataggtt atccgtacgt ggactggttt ctttctgatc cggctggagc cgtgtatgaa
     5281 tagccggcca gccaaatact gcttttaccg gacctttttt tccatgaatc agtacccggg
     5341 aggccggagc ggtctggtga tcgacgcctc cgtttctttt caggtagatc aatccatcgg
     5401 cggttatata gtttacaaac cagctgatct catcggcatg ggcttcaatg actattttaa
     5461 aaggatgatc cgcattgatc actcccacgg cggtaccata tggatccaca taatggctgt
     5521 ccacgtaggg cttcaggtac tcgatccata atttctgtcc ccaggtttca aaacctaccg
     5581 gggaggcgtt attgatataa ctctttaaaa agctataaga ttggtccgta atgattgatt
     5641 ttttagcttt tgccgctttt gccatgggta ttgtattaat caggttcaaa aataattaaa
     5701 tacgggtgaa taaagtcagt agggcggaaa aggccatcag tccgtccagt acaaaataat
     5761 agagcatatc gctgaaatcc cgggttgcac gccgatacag gccttccaca ataaacccgg
     5821 gtatcaagat caggagcacc gattctgcgg aaaggcgctg accgtaaagt ccaagggtcg
     5881 acaggataaa aatcaggaac gagaacagga atagccgtct gattcccgtg ggttccatat
     5941 acgttatcag gctgcggatg cctgcgtttt tatcatcagt ccggtcccgg tagtcaaaga
     6001 gaatacagat catatacacg gcaaaataac ggctgatggt aaag
//
LOCUS       DAOPMW010000186         6037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15079_length_6037_cov_6.871448, whole genome shotgun sequence.
ACCESSION   DAOPMW010000186 DAOPMW010000000
VERSION     DAOPMW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15079_length_6037_cov_6.871448"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="lpxA"
                     /locus_tag="PLU11_09465"
     CDS             complement(<1..604)
                     /gene="lpxA"
                     /locus_tag="PLU11_09465"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPN59391.1"
                     /translation="MIHPHTYIHPNARLATNVKIDPFTVIHQDVEIGDGTWIGSNVTI
                     MEGARIGKNCRIFPGAVIAAIPQDLKFQGEYSNVYIGDGTTIREFVTINRGTKDREKT
                     MIGKNCLIMAYCHFAHDCFVGDNCITSNNTQVAGHVTIGDWAIIGGMCAVHQFVKIGA
                     HSFISGGSLVGKDVPPYIKAGRTPLSYAGVNSIGLKRRGFN"
     gene            762..1703
                     /locus_tag="PLU11_09470"
     CDS             762..1703
                     /locus_tag="PLU11_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN59392.1"
                     /translation="MHTPPDILTLAYKQNLMQDEFSMLQEREQQIPGSVQYAIQRYRR
                     HPQWNMEDTGMLVYHYTKAAPKENYLELKFCVSGNVYCRKKDTECDLCQFNASKSCME
                     RVESIDVVSFRFSPTHLSQFVKPRKGNAALTENILGFKHNSSFTKILPLCGKTRMVLE
                     GVLNHTYSDSLENIYINAQTQMLLLYSLDCMLGEKEIDVISCKFLANEADREKIVKAR
                     EILIQHIGEPITIKELSRKVAINECYLKKGFKELFGTTVFDFYQSQRMEHAKYLLYEK
                     GLSVTEVSMMLGYSSISHFSTAFKKHTGLKPCELLLR"
     gene            1840..2217
                     /locus_tag="PLU11_09475"
     CDS             1840..2217
                     /locus_tag="PLU11_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59393.1"
                     /translation="MRKKLAILLISLHLLGNTEAGQVFKVPQLLEHYSQHKKENPALS
                     FIDFFVMHYGGDDGTDTDNDQDQQLPCHNIHHTTVCIAYSPMTKAMPINDIYLWETRS
                     YRSHLSGPIPDLPLPVLLQPPGL"
     gene            2350..3084
                     /locus_tag="PLU11_09480"
     CDS             2350..3084
                     /locus_tag="PLU11_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPN59394.1"
                     /translation="MKRKFIYAIGLLSAGLFILNACQHEILYPGNGGGGTGGGGTGGT
                     GGGGTGAVNTCSPDTVYFVNDIMPLISSNCTMSGCHDAITHADGVDLTSYTKIMRYVS
                     AGNASGSKLYKEIIKTGSERMPPPPMPAFTTAQKTMVQKWINQGAKNNSCAGRCDTAT
                     FTYSGAIKPMMDAKCTGCHNPGNLGGNIDVSTHAALKVIALNGKLYGSVAHQPAFSAM
                     PKGAAKMSDCEITQVQKWITAGSLNN"
     gene            3095..3478
                     /locus_tag="PLU11_09485"
     CDS             3095..3478
                     /locus_tag="PLU11_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59395.1"
                     /translation="MKMKLSLTALIIGTMVMISSCYYDKADLLYSGNAGPCTDSAGTV
                     SYAQKVVPLLQQYCYSCHTGNFPSGNQLMGTYTSDKAMAMNGMLYGTIAHSAGYSPMP
                     QGMAKMTNCQIAVIKKWIDSGTLNN"
     gene            3581..4033
                     /locus_tag="PLU11_09490"
     CDS             3581..4033
                     /locus_tag="PLU11_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59396.1"
                     /translation="MKRKKIIIWTIVILLIAGAGGGYYAYSEYNRKQKDTADLKADFT
                     APATALLDEYMKDEKTANAKYFGKVLKIDGLVKDLIKDDKGYYSVVLGDTASMSSVRF
                     SMDSIHNAQAATLVKGSKAVLKGICTGYNADEMLGSDLILNRAVLEKN"
     gene            4064..4621
                     /locus_tag="PLU11_09495"
     CDS             4064..4621
                     /locus_tag="PLU11_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPN59397.1"
                     /translation="MKQYFLMATAALLLTMTTSAQKYFTKTGRITFDATTSSSPEKIE
                     GVNKTATCVLDTKSGAMQFAVLMKGFLFERALMEEHFNENYVESNKFPKAEFKGSISN
                     NDAVNYSKDGTYTVTVKGKLTMHGVTKDVETTGKLTVTGGKIQAVASFNAMLSDYSVS
                     IPGLVADKVAKTAKIDVVCSLEPLK"
     gene            4645..5544
                     /locus_tag="PLU11_09500"
     CDS             4645..5544
                     /locus_tag="PLU11_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HPN59398.1"
                     /translation="MNKLVQGLLGLTLLFGSTNLLAQEEDMLKLLGEEKPKKERIKYA
                     FKSPRVINGHSMEFLNPGTMDFRILHRFGQLDKGYKNFFGLDQASMRMGFDFGILQNL
                     MVGIGRSTYRKEVDAFLKYAPIRQSTGPWHSPVTLAFVSGITMDGLDWADPTRKNFFT
                     SRLGFYFEAIIGRKFSEAFTLQIVPTMVHQNLVPLETDPNNIYALGVGGRLKLSQRVA
                     FTWDYSHVLIGMPDSGYYHPLSVGFDIETGGHVFQLHFSNATGMNERAFITETTGRWG
                     KAEVRFGFNLSRVFQIKKPKHKE"
     gene            5636..>6037
                     /locus_tag="PLU11_09505"
     CDS             5636..>6037
                     /locus_tag="PLU11_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59399.1"
                     /translation="MKKNLLLATFTVAIASLFAIQACQHEIPGDPCSKNPIFQSVSKT
                     DATGASSNGTISATASGGADFRYSLNGGAFQDTGYFSGLAPFQAYNVVVKNSWGCSDT
                     AQVSINANNPCSGVTISVTAAKTDASPGTNNG"
BASE COUNT         1750 a         1388 c         1357 g         1542 t
ORIGIN      
        1 cattgaaacc ccggcgtttg agtccgatgg agtttacccc ggcataacta agtggcgtac
       61 gtccggcctt gatataaggc ggtacatctt tacccaccag tgaaccaccg ctgataaaag
      121 aatgagctcc gatctttaca aactggtgta cagcgcacat gccaccgata atcgcccagt
      181 cgccaatggt cacgtgacca gctacctggg tattattact ggtaatacaa ttatcgccta
      241 caaaacagtc atgggcaaaa tggcaatacg ccataatcag gcagtttttc ccgatcatcg
      301 tcttttcccg gtccttggta ccccggttaa tggttacaaa ctcccggata gtggtaccat
      361 cacctatata tacattggaa tattctccct ggaatttcag gtcctgcgga atggccgcaa
      421 taaccgctcc ggggaaaatc cggcagtttt tgccaatccg tgcaccttcc ataatggtta
      481 cattggaacc aatccaggtg ccatcgccga tctctacatc ctggtgaatg accgtaaatg
      541 ggtcgatttt cacattggtg gccagccggg cgttggggtg tatatatgta tgaggatgaa
      601 tcattcagca atagttccgc cagtagcgga cgggcaaagt tagcttttag cggctaaaac
      661 agaagttttt ttacgcatat attgttaaaa atcagtacct gatcaggatc agccggtgtt
      721 acaccatcaa tatcctcagg aagcagtaaa tttgcacttt aatgcataca cctccggaca
      781 tattaaccct ggcctacaag caaaacctga tgcaggacga gttttcgatg cttcaggagc
      841 gggagcaaca gattcccggt tcggtccagt atgctatcca acgctatcgc cgtcatcccc
      901 agtggaatat ggaagatacc ggtatgttgg tgtatcatta tacaaaagca gctccgaagg
      961 aaaattacct ggagttgaaa ttctgtgtaa gcgggaatgt gtattgccgg aaaaaagaca
     1021 cagaatgtga tctctgtcag ttcaatgcct ccaaatcctg tatggaaaga gtagaaagta
     1081 ttgatgtggt aagttttcgg ttcagtccca cacacctgag ccagtttgta aaaccccgaa
     1141 agggcaatgc tgctctgaca gagaatatcc tcggttttaa acacaattca tcctttacaa
     1201 aaattctccc gctttgcggc aagacccgca tggtattgga aggagtgttg aatcatacat
     1261 acagcgacag tctcgagaat atctatatca atgcacaaac ccagatgctg ttactctatt
     1321 cactggattg catgctgggc gaaaaagaaa ttgatgtgat cagctgcaaa tttctggcga
     1381 atgaagctga ccgggaaaaa attgtcaaag cccgcgaaat cctcatccag catattggtg
     1441 aacccatcac tattaaagaa ctcagccgta aagtggctat caatgaatgt tacctgaaaa
     1501 aagggttcaa ggaattattt ggaactaccg tgtttgattt ttatcaaagt cagcggatgg
     1561 aacacgccaa atacctgctc tatgagaagg gtttaagtgt aacggaagta tccatgatgc
     1621 tgggctactc ttcaatatct cacttctcca cggcctttaa aaagcataca ggccttaaac
     1681 cttgtgaact tttacttaga taagtaaggg actattcacg aatggttatt cgttaaaccc
     1741 gttataaact gcggctagta cttattttta agcaataaga tttatgtgac aggcgttacg
     1801 catcatatgg catcattttt ggatatttga ttgtcacgaa tgcgtaaaaa actggcgata
     1861 ctcctgatta gtctgcacct cctcggaaat accgaggcgg gacaggtatt taaggtacct
     1921 cagttactgg agcattatag tcagcataag aaggagaatc cagcacttag ttttattgat
     1981 ttctttgtaa tgcattatgg cggagatgat ggcacggata ctgacaatga tcaggatcaa
     2041 caattgccct gtcataatat tcatcatact actgtttgca ttgcttattc tcccatgaca
     2101 aaggcaatgc ccatcaatga tatttactta tgggaaaccc ggtcttaccg ttcacattta
     2161 tccggcccta ttcctgactt accattaccc gttttattac aaccaccggg attgtaagca
     2221 tcaccgcttc ctttcacctt acactttttc gtacgatcag gttacataca gttatctgta
     2281 gctgactaag tctgcattgc tgttaatgac cggtatctgt acccattttt ttaaatcaaa
     2341 tcaacgatta tgaaacgcaa attcatttat gcaatcggat tgctgagtgc cggcctgttt
     2401 atcctcaacg cctgtcagca tgaaattctt tatcccggaa atggcggcgg tggcaccggt
     2461 ggtggtggaa ccggtggtac aggcggtgga ggtaccggtg ctgtcaatac ctgcagtccg
     2521 gatacggtct attttgtcaa tgatattatg cccctgattt cctccaactg taccatgagc
     2581 ggatgccatg atgccatcac gcatgcggac ggcgtggatc tgacatcata tacaaagatc
     2641 atgcgttatg tatccgctgg caatgcttcc ggtagtaaac tgtacaaaga gatcataaaa
     2701 acaggtagtg aacggatgcc cccgcctccg atgccggcat ttaccacggc acagaaaacc
     2761 atggtacaga aatggatcaa ccagggtgcg aagaacaaca gttgtgcggg tcgttgtgat
     2821 accgccacat tcacttatag cggtgctatt aaacccatga tggatgcaaa atgtaccggt
     2881 tgccataatc cgggtaacct gggaggcaat attgatgtgt ctacacatgc cgcgctgaaa
     2941 gttatcgcgt tgaatggaaa attatatggc tccgtggcac atcagccagc tttttctgcc
     3001 atgccaaaag gtgcagcaaa aatgtctgat tgtgaaatta cccaggtaca aaaatggatc
     3061 acagccggct cactcaacaa ttaaaatgaa cagcatgaaa atgaaactat ctcttacagc
     3121 attaatcatc ggaaccatgg taatgatcag ttcctgctat tacgataagg cagatttgtt
     3181 atatagtggc aatgccggcc cctgtacaga ttctgccggc acggtttcct atgcacaaaa
     3241 agtagtgccg ctgctgcagc aatattgtta cagctgtcat accggtaatt ttccttccgg
     3301 taatcagctc atggggacgt atacatcgga caaggcaatg gccatgaacg gaatgctata
     3361 tggtaccatc gcccattcgg ccggttattc acccatgcca cagggtatgg caaaaatgac
     3421 gaattgccag atcgccgtta ttaaaaaatg gatcgatagc ggtacgctga ataattaact
     3481 aaatcaccct tcgacacgct ctccgcatat gacgggacaa gcaggatggc aacaccactt
     3541 gtcatggtga gcctgccgaa ccacaaaaca ccatcataca atgaaacgta aaaaaataat
     3601 catctggacg atcgtcatct tgctcatcgc aggtgcaggt ggcggctact atgcttacag
     3661 tgaatataac cgcaagcaaa aagataccgc tgatctcaaa gccgatttta cggcaccggc
     3721 cactgctttg ctggatgaat acatgaaaga tgaaaaaaca gcgaatgcaa aatacttcgg
     3781 caaagtgctg aagattgacg gactggtgaa agacctgatt aaggatgaca aaggatatta
     3841 ttcagtagta ctgggcgata cggcatcgat gtcgtcggtc cggttcagca tggacagtat
     3901 tcataatgca caggccgcaa cgctggtaaa aggatcaaaa gcggtattaa aaggaatttg
     3961 taccggctat aacgcggatg aaatgctcgg ttcagatctg atcctgaaca gggccgtact
     4021 tgaaaagaac taaaaacata caaacaacac ctaaatcatg aacatgaaac agtatttttt
     4081 aatggcaaca gcggcactgc tgctgaccat gaccacttca gcgcagaaat attttaccaa
     4141 gacaggccgt atcacatttg atgcgaccac ctcttcttcg cctgagaaaa ttgaaggcgt
     4201 gaacaagacc gccacctgtg tcctggatac aaaatccggt gctatgcagt ttgcggtgct
     4261 gatgaaaggc ttcctcttcg aaagagcact gatggaagaa cacttcaatg aaaactatgt
     4321 ggagagtaat aaatttccca aagcagaatt taaaggcagc atcagtaata atgacgccgt
     4381 gaattacagc aaggacggca cctataccgt aaccgttaaa ggaaaactga ccatgcacgg
     4441 ggtgacaaag gatgtcgaaa caaccggtaa gctaaccgtt actggcggga aaattcaggc
     4501 agtggcttcc tttaatgcga tgctttcaga ttatagcgtg tccatacccg ggctggtggc
     4561 agacaaagta gcaaaaacag ccaaaattga tgtggtctgt tccctggagc ctttaaaata
     4621 attaaacgaa aaattcaatg cactatgaat aagctggtac aaggcctcct gggactcacc
     4681 ctccttttcg gctcaacgaa cctgttagca caagaagagg atatgctcaa gctcctgggt
     4741 gaagagaaac ccaaaaaaga gcgcattaaa tatgcattca aatcaccccg cgtaataaac
     4801 gggcattcca tggaattcct gaatccggga accatggact tccggatcct gcaccgcttt
     4861 ggtcagctgg ataaggggta taagaatttc ttcggactcg atcaggccag tatgaggatg
     4921 ggatttgatt ttggcattct gcaaaacctg atggtgggca ttggccgcag cacctacaga
     4981 aaagaagtgg atgccttttt gaaatatgcc ccgatccggc aatccaccgg tccctggcac
     5041 tcaccggtta ccctggcgtt tgtatcgggc atcaccatgg atggactgga ttgggcagat
     5101 cccacccgta agaatttctt tacctccagg ttgggttttt attttgaagc catcatcgga
     5161 cgaaaattca gtgaggcatt taccctgcag attgttccga cgatggtaca ccagaacctg
     5221 gtgcccctgg aaacggatcc caataatatc tatgcattgg gagtgggcgg tcgcctgaaa
     5281 ctttctcagc gggtggcctt tacctgggac tattcgcatg tgttgatcgg catgccggac
     5341 agtggctact atcatccctt gtcagttggt tttgatatcg aaacaggcgg tcacgttttt
     5401 cagctgcatt tttccaatgc gacaggcatg aatgaaagag cttttattac tgaaaccacc
     5461 ggaagatggg ggaaggccga agtccggttt ggattcaacc tgtccagagt atttcagatt
     5521 aaaaaaccca aacacaaaga ataaacagtt tttacagcca cagcaagttt ttctccttac
     5581 gaaccactta acaaaagaca ccgaagagta ttacatcatc aaaaaaaagt gatttatgaa
     5641 gaaaaacctg ctgctggcaa catttactgt tgccattgcc tccctgtttg ctatccaggc
     5701 ctgtcaacat gaaattcccg gcgacccctg ttccaagaac ccgatttttc aatctgtatc
     5761 taaaaccgat gcaaccggag cctcctccaa cggcaccatt tccgccactg catccggtgg
     5821 tgctgatttc aggtacagtc tgaatggcgg cgcttttcag gataccggat atttttccgg
     5881 actggcccct ttccaggcct acaatgtagt ggtgaaaaat agttggggtt gttcagatac
     5941 cgcacaggta tctattaatg cgaacaatcc ttgttcaggg gttaccattt cagtaacagc
     6001 tgccaaaaca gatgcatcac cgggtaccaa taacgga
//
LOCUS       DAOPMW010000187         6026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15120_length_6026_cov_6.718808, whole genome shotgun sequence.
ACCESSION   DAOPMW010000187 DAOPMW010000000
VERSION     DAOPMW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15120_length_6026_cov_6.718808"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2762
                     /locus_tag="PLU11_09510"
     CDS             <1..2762
                     /locus_tag="PLU11_09510"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPN59400.1"
                     /translation="NSHPEYIENLYRDFLKDPSSVDPEIKKFFEGFDFAMSNGANLNG
                     KTAAPATTASETAPAIAATTGSSDNTDWLRELRAYRMILGYRNKAHLIAKTNPIRTRK
                     DRGAHIELAFYGFSEKDLDTSFQVGELIGLGTTSLRSILAHLEKCYASSVGVEFKYIS
                     DPKKVDWLTREIETTLLQPLPLAKKKRALEKLNQGVMFEKFLHTKYVGQKRFSLEGGE
                     TTIAALDAIISAAADQDVHEVVIGMAHRGRLNILANIMGKTYEQIFSEFEGTAKVDQT
                     MGSGDVKYHMGYGSEVETINNKTIHLKLMPNPSHLEAVNPVVVGFARAKADVMYESEF
                     DRILPILIHGDASVAGQGIVYEVLQMSYLRGYYTGGTIHYVINNQIGFTTDFDDARSS
                     DYCTSLAAAVQAPVFHVNGDDPEAVIKCAEIATRYRQEFNGDVFIDMVCYRRHGHNEG
                     DDPKFTQPHLYALIDKHPNPREVYIKYLLENGEPDAQELAKAMEKKFWADLQERLDEI
                     KQNPLPYHYQAPELEWKKLRRAVVNDFDRSPSTSISEDKFNTVFKAMMSWPEGFKPLR
                     KVEKILQDKVKLFETEHKVDWATAELLAYGSLTLEGKDVRMSGQDVRRGTFSHRHAVL
                     RDEQNDKPYSRLSKIPGKTGKFRIYNSLLSEYGVLGFEYGYALANPNALVIWEAQFGD
                     FANGAQTMIDQFIAAGEQKWNRMNGVTLLLPHGYEGQGPEHSSARLERFLQLCAELNM
                     VVTNITTAANLFHALRRQLAWPFRKPLINFSPKANLRHTGTYSRLEEFVNGGFKEVID
                     DEKADPVEVRKVLFCSGKMYFDLAERQQKENRKDVAIIRLEQIYPLPHKQLEALYKKY
                     NKATWFWVQEEPLNMGAASFLKMNLTQLNYGVISRQPSASTATGYNKVHLQEQGEIIE
                     TAFSI"
     gene            2779..3996
                     /gene="odhB"
                     /locus_tag="PLU11_09515"
     CDS             2779..3996
                     /gene="odhB"
                     /locus_tag="PLU11_09515"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPN59401.1"
                     /translation="MIEIKVPTVGESISEVTLLKWVKKDGDYVERDEVIAELESEKAT
                     FEVNAEKAGVLKTAAAEGDSLKIGDLLASIDESAEKPAGKAAEPATAPAKQEAVPSAA
                     PVTAVPNDIKATPVASAIIADKKVDPKTVTPTGAMGKIMKDDVLAALSAPGKKAFAGT
                     ELNSRNVRVEKMSNLRKTISRRLVEAKNTTAMLTTFNEVDMTAIMEIRTKYKDKFKEI
                     HGVGLGFMSFFAKACAVALGEWPSVNAYIDGDQLVYHDYADISIAVSTPRGLTVPVMR
                     NVESMSMADVERKVVELAGKARDSKLTTEELTGGTFTITNGGVFGSLMSTPIINIPQS
                     AILGMHKIQDRPMAVAGQVVIRPMMYIALSYDHRIVDGRESVSFLVRVKELLENPEQL
                     LIGKDPVKTLLEL"
     gene            4064..5230
                     /locus_tag="PLU11_09520"
     CDS             4064..5230
                     /locus_tag="PLU11_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HPN59402.1"
                     /translation="MSRYHSYLNSSITLLQLYKGEEPFAAFLKKYFAANKKYGSKDRK
                     QIAHCCYCFFRAGHILKELSREEQVLSALFLCSQVSDPVLAALKPEWEESYALSPSEK
                     LKLLHIKKPLHELFPWSNELSEGIEPDEFAASFLLQPDLYIRIRPGYHEVLKSKLKAS
                     GVNFHEISETCFSFPSGTKLDEYLSTNKEAVIQDYNSQQVGELMSLVKREGWINVWDA
                     CAASGGKSIMAKDRLGKINLTVSDIRESILHNLKKRFAEAGISDFQTVIADLSDNDPL
                     QPGDQFDLVIADVPCSGSGTWGRTPEQLFYFEEKKVKEYSHLQQKIVSKLLPHVKPGG
                     FLLYITCSVFKKENEQQADFIFADKAFTLLRKEWLNGYDKKADSMFAALFCREL"
     gene            complement(5225..>6026)
                     /locus_tag="PLU11_09525"
     CDS             complement(5225..>6026)
                     /locus_tag="PLU11_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59403.1"
                     /translation="TNNGTSFACPNMAGLGTCLWQGFPEYNNMKIVQALKAAGSKYSN
                     PDNRIGYGIPNMKLAFTNLLTEFATATASTSACTATVNWTSKDVSAMKYEIERMGPGE
                     TVYTKVGEMNPQAGNLLAIRSYQFNNSLNGLNAGTVSYRIRQIVDTAIASFTAVYIDT
                     TTTTVAAGCFATGTGNTDPVNEKITIQPNPVNGSTLILVIETPNAIPSMPVHIYDAKG
                     ALVWQEKNSKSSGKKTIELNIAGLPAGKYYISVFNGKKLIRTAELIRL"
BASE COUNT         1669 a         1326 c         1531 g         1500 t
ORIGIN      
        1 ctaactccca cccggagtat attgaaaacc tgtaccgtga ttttctgaag gacccgtcct
       61 ctgtggatcc tgaaatcaaa aagtttttcg agggatttga ttttgccatg agtaatggcg
      121 ccaacctcaa tggtaaaaca gcagctccgg ccacaacggc ttccgagaca gccccggcaa
      181 ttgctgctac aaccggatct tccgacaata ccgattggct ccgcgaactc cgggcttacc
      241 ggatgatcct gggttatcgg aacaaagccc acctgattgc caaaaccaat ccgatacgta
      301 cccgtaaaga ccggggcgcc catattgaat tagcttttta cggattttct gaaaaagatc
      361 tggatacatc cttccaggtt ggtgaactga ttggattggg tactacttct cttcgtagta
      421 ttctggctca cctggaaaaa tgttatgcat ccagtgtggg tgttgaattc aaatacatca
      481 gcgatccgaa aaaagttgat tggctgaccc gtgaaattga aactacttta ctgcaacccc
      541 tgccactcgc gaaaaagaaa cgagccctgg agaaactgaa ccagggagtg atgtttgaga
      601 aattcctgca taccaagtat gtaggtcaga agcgtttttc actggaagga ggggagacta
      661 ctattgccgc cctcgatgca attatcagtg ctgccgcaga tcaggatgtc catgaagtgg
      721 tgattggaat ggcacaccgg ggtcgactga atatcctggc caatatcatg ggtaagacct
      781 atgaacaaat cttcagcgag tttgaaggca ctgccaaagt ggatcagacc atgggcagcg
      841 gcgatgtgaa ataccatatg ggttatggtt ctgaagtgga gacgatcaat aataaaacca
      901 tccacctgaa actcatgccc aatccctctc acctcgaagc agtaaacccg gtggtggtgg
      961 gctttgcccg ggccaaggcc gatgtgatgt atgagagtga gtttgaccgc atccttccga
     1021 tcctgattca tggcgatgct tcggtggccg gtcagggaat tgtttatgaa gtattacaga
     1081 tgagttacct caggggatat tataccgggg gaaccattca ctatgtgatc aataaccaga
     1141 ttgggttcac taccgatttt gatgatgcac ggagctcgga ttattgcacc tcccttgcgg
     1201 cggctgtcca ggcaccggta tttcacgtaa atggcgatga tccggaagcg gtgattaaat
     1261 gcgcggagat tgccacccgt taccgccagg aatttaacgg cgatgtgttt attgatatgg
     1321 tatgttaccg tcgtcacgga cacaatgaag gcgatgatcc caagtttact caaccgcatt
     1381 tgtatgcctt gattgacaaa caccccaacc caagggaagt ctatattaaa tacctgctgg
     1441 aaaacggaga acccgatgca caggaactgg ccaaggccat ggagaaaaaa ttctgggcgg
     1501 acctgcagga acgacttgat gagatcaagc aaaatccatt gccttatcat taccaggcac
     1561 ctgaactgga atggaaaaaa ttaagaaggg ctgtggtcaa tgattttgac cgatctccct
     1621 caaccagtat atccgaagat aaattcaata cggtattcaa agccatgatg tcatggcccg
     1681 aaggattcaa gcccttgcgt aaagtggaaa agatcctgca ggacaaagtg aaattatttg
     1741 aaacagaaca taaggttgac tgggctacag ccgaactgtt ggcttacgga agtctcacac
     1801 tcgaaggcaa agatgtacgg atgagcggac aagatgtacg tcggggtacc ttctcacacc
     1861 ggcatgctgt actccgtgat gaacagaacg acaaaccgta tagccgttta tcaaagatcc
     1921 ctggtaagac tggcaaattc aggatttata attcattact gagtgagtat ggtgtgcttg
     1981 ggtttgaata tggctatgca cttgccaacc cgaatgccct ggtgatctgg gaagcccagt
     2041 ttggcgattt tgccaatggc gcccaaacca tgatcgatca gtttattgct gcgggtgaac
     2101 agaaatggaa ccggatgaac ggtgtgacct tattacttcc gcatggatac gagggtcaag
     2161 gtccggagca cagcagcgcc cgcctggaac gattcctgca attatgtgct gaattgaata
     2221 tggtggttac caatatcaca actgcggcca atctgtttca cgctttaaga agacagctcg
     2281 cctggccttt ccgtaaaccg ctgatcaact tttcaccaaa ggccaatctt cgtcacaccg
     2341 gcacctattc ccgcctggaa gaatttgtca atggcggctt taaagaagtg atcgatgatg
     2401 aaaaagccga cccggttgaa gtaagaaaag tacttttctg cagcggaaaa atgtattttg
     2461 atctggccga acgccagcag aaagagaacc ggaaagacgt agccattata cgactggagc
     2521 agatctatcc attaccccat aagcaactgg aagccttgta taaaaaatac aacaaagcca
     2581 cctggttctg ggtgcaggaa gaacccttga atatgggggc cgcttccttc ctgaaaatga
     2641 acctgaccca gttgaattat ggcgtgatca gccggcagcc atcggcttcc accgctacgg
     2701 gttataataa agttcacttg caggaacagg gagagatcat tgaaaccgca tttagtatct
     2761 gattgaataa ataatattat gattgaaata aaagtaccaa ccgttggaga atcaataagc
     2821 gaagtgacct tgttgaaatg ggtgaagaag gatggcgact atgtggaacg tgatgaagtg
     2881 attgcagaac tggagagtga gaaagccact tttgaagtga atgcggaaaa agcgggcgtt
     2941 ttaaaaacgg ctgcagcaga aggtgattcc ctgaagatcg gggacctgct cgccagtatt
     3001 gatgaaagtg cggagaagcc tgcaggtaaa gcggcagaac cggcaacggc tccggctaaa
     3061 caggaagcgg taccttctgc ggcaccggtt acggctgtac ccaatgacat aaaagccact
     3121 cccgttgcct cagcgattat agcggataaa aaagtagatc ccaaaaccgt gacaccaacc
     3181 ggcgcgatgg gcaaaatcat gaaggatgat gtgctggcgg ccttatccgc ccctggtaag
     3241 aaagcctttg ccggtacgga actcaacagc cggaatgtac gggtggagaa gatgagtaac
     3301 ctgcgtaaaa ccatttcccg ccgcctggtg gaagcgaaga ataccacggc gatgctcact
     3361 acgtttaacg aagtggacat gaccgccatc atggaaatca ggactaaata caaagataaa
     3421 ttcaaagaga tccatggggt tggacttggc tttatgagct tttttgccaa agcctgtgcc
     3481 gtagccctgg gtgaatggcc ttcagtgaat gcctatattg atggtgacca actggtgtat
     3541 catgattatg cagatatcag tattgcagtc agtacacccc gcgggttaac cgtaccggta
     3601 atgcggaatg tggagagtat gagcatggcg gatgtagagc gcaaagtagt ggaactggcc
     3661 ggtaaggccc gtgacagtaa actcactacc gaagaattga ccggtggtac gtttaccatt
     3721 accaatggag gtgtattcgg ttccctgatg agcaccccga ttattaatat accccagagt
     3781 gccattttgg gtatgcataa aatacaggac cgcccgatgg cagtcgccgg acaggtggtg
     3841 atccgtccca tgatgtatat cgccctcagc tatgatcacc ggattgttga tggaagagaa
     3901 tccgtgagtt tcctggtaag ggtgaaggaa ttgctggaaa atcccgaaca actgctgatt
     3961 ggaaaagatc cggttaagac attacttgaa ctctgatcaa tcagaagaac agataaaaag
     4021 aaaggactgc agggatgcgg tccttttttg ttttctttgc agggtgagcc gttatcattc
     4081 ttacctcaac tcgtccatta cgctccttca gttatataaa ggagaagaac cctttgcggc
     4141 ttttctgaaa aagtattttg cggcgaataa aaaatacgga agtaaggacc ggaaacaaat
     4201 agcccactgc tgttattgtt ttttccgggc cggacatatc ttgaaagagt taagcaggga
     4261 agagcaggtg ttgtccgcct tattcctctg ctcacaggta tctgatccgg tacttgctgc
     4321 attgaagccg gaatgggagg aaagctatgc actcagcccc agtgaaaagt tgaagttgct
     4381 ccatataaaa aaaccgttgc atgaactttt cccctggtcc aatgagttaa gtgagggaat
     4441 agaacctgat gaatttgctg cttcatttct gctgcaacct gatttatata tccgcatccg
     4501 gccagggtat cacgaggtac tgaaatcgaa gttaaaggca agtggtgtta acttccatga
     4561 aatcagtgaa acctgtttct cttttccctc tggtaccaaa ctggatgaat acctcagtac
     4621 caataaagaa gcggtgatcc aggattataa ttcacagcaa gtgggggaat tgatgtcttt
     4681 ggtaaagcgt gagggctgga tcaatgtgtg ggatgcctgt gccgccagtg gcggcaaatc
     4741 gattatggct aaagaccggc tgggtaaaat caatctgacc gtatcggata tccgtgaatc
     4801 cattctccat aacctgaaga agcggtttgc cgaagcgggg atcagcgatt tccaaactgt
     4861 cattgccgat ctgtcggaca acgacccttt acaacccggg gatcagtttg acctggtgat
     4921 cgcggatgtg ccctgcagcg gaagcggaac ctggggacga accccggagc agttgtttta
     4981 ttttgaggaa aagaaagtga aggaatacag ccacctgcaa caaaagatcg tttcaaaatt
     5041 attgccgcat gttaagccgg gtggtttttt gctttatatc acctgctccg tatttaaaaa
     5101 agaaaatgaa cagcaggcag attttatttt tgcggacaaa gcttttacat tgctccgtaa
     5161 agaatggctg aatgggtatg ataaaaaagc ggattcgatg tttgccgctt tgttttgcag
     5221 ggaattataa acggatcagt tccgcagtcc ggattagttt tttcccatta aacacactga
     5281 tataatattt ccccgcgggt aatccggcga tgttgagttc gattgttttc tttccgctgc
     5341 ttttgctgtt tttctcctgc catacaaggg cgcctttcgc atcataaata tgaaccggca
     5401 tagaaggaat ggcattcggt gtttcgataa ccaggatgag ggtactgcca tttaccggat
     5461 tgggttgtat cgtgatcttt tcgtttacag gatctgtatt tccggtgccg gtggcaaaac
     5521 agccagcggc aacagtggta gtggtggtat cgatatacac agctgtgaag ctggcaatgg
     5581 cggtatctac gatctggcgg atgcgatagg aaaccgtacc cgcattgagt ccgttcaggg
     5641 aattattaaa ctggtaagag cggatcgcta ataagttgcc agcttgtgga ttcatttcac
     5701 ctactttggt ataaaccgtt tcgccaggac ccattctttc gatttcatat ttcatggccg
     5761 aaacatcctt gcttgtccag ttaaccgtag ccgtacaggc gctggtgctg gcggtggctg
     5821 tagcaaattc cgtaagcaga ttggtgaacg ccagtttcat attcgggatg ccataaccaa
     5881 tccggttatc cggattactg tatttactcc cggccgcttt cagggcctgt acaatcttca
     5941 tgttattgta ttccggaaag ccctgccaga gacaggtgcc aagtccggcc atattgggac
     6001 aagcgaagga agtgccgtta ttggtg
//
LOCUS       DAOPMW010000188         6017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15157_length_6017_cov_9.151962, whole genome shotgun sequence.
ACCESSION   DAOPMW010000188 DAOPMW010000000
VERSION     DAOPMW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15157_length_6017_cov_9.151962"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLU11_09530"
     CDS             complement(<1..812)
                     /locus_tag="PLU11_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59404.1"
                     /translation="MKPSESALFAPYIVWVLAPHLESEDPNIQYYYDFSQSIKEYTRV
                     FEELNVEWKWQPVTMDNFQDIIGSIQTNSPGKTPLILNLCDGDEVNGTPGVSVIHEIE
                     KNGLIYTGSDAHFYDITTSKIPMKKAFDKAGVATANWRVITEKKGTVKGICKRVGIPL
                     IIKPAVSGGSMGVSVKNVVNSEEELAERVEEIKKGYRGWNLLADGLFVEQFITGPEYT
                     TFITGSANDPEHCIVYEPVERVFHPSLPDAEKFLSFDRLWEIYEDEAPMPEEG"
     gene            complement(817..1323)
                     /locus_tag="PLU11_09535"
     CDS             complement(817..1323)
                     /locus_tag="PLU11_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPN59405.1"
                     /translation="MDSSSTPGYRPVSNHVIAEVRQHISSGQNALFTLRSWQPGEVIA
                     DFSAGTISAEPTYLTVQIAEGKHITLQPEFLQYINHSCEPNVFFDTTLMQLVALKDIP
                     AGTEFAFFYPSSEWEMTQSFQCYCGKPGCLGEIRGAAHLSSEAVSQHRFTDFIQSRLA
                     KKADKKVA"
     gene            1589..3007
                     /locus_tag="PLU11_09540"
     CDS             1589..3007
                     /locus_tag="PLU11_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN59406.1"
                     /translation="MYAQCAQPINSFPYQESFETSNGGWTTAGNSSTWQWGTPAKAVI
                     NQAGDGTRCWVVGGLAAGSNYGSGEQSWLQSPCFDFTNLQYPLLTMKLFWETEQQWDG
                     ASFQYSLDDGLSWTTLGVANERQDCFNKNWFNTPGINGLNGMAGTSGSTHGWTGNNLA
                     NNGSCRGGSGSKTWVQVSRTLPSLGGESSVVFRFIFGAGTTCNNYDGFAVDSINISAA
                     PPNDAAFTFTCNANRTIQFTNTSAFCPTSFRWEFDDPLSSGNNTSGLANPTHTYAFPG
                     NYTVTLTVDGPGNLYSTTTRDILIVDVNVAQVKSAACETNTGGSLIATVRGAGTTPVN
                     YTWNTNPVQQTPVATNLGTGTYNVIVTGQDICTATDSGVVTIDSSCRELVFPSAFTPN
                     GDGLNDRFDPMGSVAAAKEYKLRIYNRWGQLVYSSKNPFEQWDGRPSETTGMNALYTW
                     YCEYILPGQAKSAKKGTLMLIR"
     gene            complement(2996..5530)
                     /locus_tag="PLU11_09545"
     CDS             complement(2996..5530)
                     /locus_tag="PLU11_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN59407.1"
                     /translation="MSGFKIDISNQFFILAADLINQTDRTIFLTGKAGTGKTTFLKYI
                     REHCTKQMAIVAPTGVAAINAGGVTIHSFFQLPLAPFVPVARGQGFAPAGQEISNPHS
                     LVSRLRFNGDKKKVIQQMELLIIDEISMVRADILDAIDTVLRHIRRKPRERFGGVQLL
                     FIGDMFQLPPVVKEPEWSLLSPYYNSPYFFDSLVLKEAPPLYIEFDKIYRQREADFIS
                     LLNQVRNNELNPEGKAILESRFQPGFRRSKDDGYIILTTHNEQARNINNQQLNQLTSP
                     LVQYEAELENDFPPNAFPAEQTLVLKEGAQVMFIRNDTSDKGKRYYNGKIGTVTRLEE
                     AKIYVRCETDGEEIEVIRDKWENIRYSLDKTSQSMKEELLGSFTQFPLRLAWAITIHK
                     SQGLTFEKAIIDAGEAFAAGQVYVALSRCTSLSGMILRSKVRTNSLFTDPRIVEFTRR
                     SASSDLLQTELKASRRSYQLKMLTAAFSFDRQAGEVIELKKFILDNTGSFTKEARAWT
                     DELLVKLNTLQDTALRFHQWLAGQFQSDLPPEENTVLLERLQKASEHFTQEAESVITF
                     IQQPSMVTDSRQHSKEVNDLLKDLFAGLSLAIHLYKGFNGSLDTDTWHQRRRSFILPH
                     FSVNVYAGVSSAVTDSSTHPDLYHALKKQRDAFCAQKDLPVYMVASGVTLGEMCLYLP
                     HDLQEMRKISGMGEVRTAQYGQDFLDIILDYCQQHELGSLIHAKGEKRERKAKKEKPI
                     RKGDTYLASLELFKQGLSPQEIAESRNLALSTIEGHLTRFVSSGDIDIASLVAPGKLS
                     QIEEVLNKAEGYALGPLKQVLGEEVSFGEIRWVLAAKGLDRVTSTD"
     gene            complement(5615..>6017)
                     /locus_tag="PLU11_09550"
     CDS             complement(5615..>6017)
                     /locus_tag="PLU11_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPN59408.1"
                     /translation="ISWHFYQIMTDILYKEEVYTIIGYCMEVWRTLGYGFSEVVYKDA
                     MELEFIEHNIPYLREEDLFVFYKTNKLKHRFRSDFTMYNKIIVEVKANEVATFSGHFE
                     QTINYLRASDFRLGVIVNFGKKRLEYKRIIV"
BASE COUNT         1582 a         1425 c         1369 g         1641 t
ORIGIN      
        1 ccttcttccg gcatgggcgc ttcgtcttca tagatttccc atagacggtc aaaagagagg
       61 aacttttccg catccggaag agaaggatga aagacccgtt caacgggttc gtatacgata
      121 caatgctcgg ggtcattggc agaaccggta ataaaggtgg tgtattcggg tccggtgatg
      181 aattgttcta caaataaacc atcggccagc aggttccagc cccggtaacc ctttttgatc
      241 tcttccactc tttcggccag ttcttcctcg gagttcacca catttttaac agatacaccc
      301 atgcttcccc cggaaacggc aggtttaatg atcagcggga ttccaaccct tttgcagatt
      361 cccttaacgg tacccttttt ttctgtaatt accctccagt tggccgtcgc cactccggcc
      421 ttatcaaaag ccttcttcat gggtatctta gacgttgtga tatcgtaaaa atgagcatcg
      481 gatcctgtat aaatgaggcc gttcttttcg atttcatgaa tgacagatac acccggtgta
      541 ccattcactt catcgccatc gcaaaggttt aatattaatg gcgtctttcc cggggaattg
      601 gtttgaatag agccgataat atcctggaaa ttatccatgg tcaccggttg ccatttccat
      661 tcaacattca gttcctcaaa tacacgcgta tattctttaa tgctttgcga aaaatcgtaa
      721 tagtattgaa tattggggtc ttcagactcc aggtgcgggg caagaaccca tactatatag
      781 ggcgcaaaca gggcggattc agatggcttc atcggctcag gctacttttt tatcggcttt
      841 tttagccagg cgggattgaa taaaatcagt gaaccggtgt tgggaaacag cttctgatga
      901 aaggtgggca gcgcccctga tttctcccag gcaacccggc ttaccacagt aacactggaa
      961 agattgagtc atttcccatt cagaagaagg gtagaagaat gcaaattcag taccggccgg
     1021 aatgtccttt aaagccacca gttgcatcaa cgtagtatca aagaagacat tgggctcaca
     1081 actatgattg atatactgaa gaaactcagg ttgcagcgtg atgtgttttc cttccgctat
     1141 ctgcaccgtt aaataggtag gttcagcgga aatagtacct gcggaaaaat cggcgattac
     1201 ttctccgggc tgccagctgc gcagggtaaa aagggcattc tgaccactgc tgatatgttg
     1261 ccgtacttcg gcaattacat ggttactgac tggccggtag ccaggggttg aagaagagtc
     1321 cataaatgag tttggtaagt attagaatga agttatagat aaagtttaat gtgacaagtt
     1381 caatgaaaat aaatgtgaat aaaggttgag taactgcaaa cggataacat tcaaatttca
     1441 ttgatgaatg gggcaacccg gaagaaggct tcaaccgttt accattcggt ttgttatctt
     1501 tatcggctta attcactgat taaattcaac gaccctgaat gcccgttcga ttatattaat
     1561 tcctttattc gtttgttata caatcagttt gtatgctcaa tgcgcccaac cgatcaacag
     1621 ctttccttac caggagagtt ttgaaaccag taatggtggc tggaccactg cgggtaatag
     1681 ttccacctgg caatggggaa ccccggcaaa agcagtgatt aatcaggcag gtgatggaac
     1741 ccgctgctgg gtagttgggg gattggctgc tggtagcaac tatggatccg gggaacaatc
     1801 ctggctgcaa agtccatgct ttgattttac caatctgcaa tacccattac tcacaatgaa
     1861 actgttctgg gaaaccgaac agcaatggga tggtgcctca ttccaatatt ctttggatga
     1921 tggtctcagc tggacgaccc ttggtgttgc caatgaaaga caggattgct ttaataaaaa
     1981 ctggttcaat acaccgggta taaacggtct gaatggcatg gccggcacca gcggaagcac
     2041 gcacggctgg accggtaata acctcgctaa taatggatcc tgcaggggag gaagcggtag
     2101 caaaacctgg gtgcaggtct ccagaacctt accgtccctc gggggagaat cttcagtcgt
     2161 gttccgcttt atcttcggag caggtactac ctgtaataat tatgatggat tcgcggtgga
     2221 ttcgatcaat atcagtgcgg cacctcccaa tgatgcggct tttacattca cctgcaatgc
     2281 caaccggact atccagttta ccaatacttc ggcattttgt cctaccagtt tccgatggga
     2341 atttgatgac ccgctttctt ccggtaacaa cacatccggt ctcgctaacc caacgcatac
     2401 ctatgccttt cccggaaact acaccgttac cctgaccgta gatggtccgg gtaacctcta
     2461 ttctaccacg accagggata tacttattgt ggatgtgaac gtggcgcagg taaaatcggc
     2521 tgcctgcgaa accaacaccg gaggatcgtt aatcgctacc gtaagagggg ccggaacaac
     2581 gcctgtcaat tatacctgga ataccaaccc ggtacagcaa actccggtag ctaccaacct
     2641 tggtaccggc acttacaatg tgattgttac cggacaggat atctgtacgg caacagatag
     2701 cggggtggtt acgattgatt cttcctgtcg tgaactggtc tttccctctg cttttacacc
     2761 gaatggagat ggcttgaatg accggtttga tccgatggga agtgtggcag cagccaaaga
     2821 atataaacta aggatctaca atcgctgggg acaattggta tatagcagta agaatccctt
     2881 cgaacaatgg gatggaagac cgtcagaaac gaccgggatg aacgctttgt atacctggta
     2941 ttgtgagtat atattacccg gacaagctaa atcagctaaa aaaggaaccc tgatgttaat
     3001 ccgttgaggt cacccgatcc agacctttag cggccagtac ccagcggatt tctccgaaac
     3061 ttacttcttc ccccagtact tgtttgagtg gtcccaatgc ataaccttct gctttgttaa
     3121 gcacttcctc aatctggctc aatttcccgg gtgcaaccag ggaagcaata tcaatatcac
     3181 cggaagacac aaaacgggtc aggtgccctt caatcgtaga taaagcaaga tttcgggatt
     3241 cggcaatttc ctgcggagat aaaccttgct taaacaactc cagcgaagcc agataagtat
     3301 cccctttcct gattggtttt tcctttttgg ccttgcgttc tcttttctcc ccttttgcgt
     3361 gtatcaatga ccccaattca tgttgctggc aatagtccag gataatatcc aggaagtctt
     3421 gtccgtattg ggctgtcctg acttctccca tcccgctgat ctttcgcatt tcctggagat
     3481 catgggggag gtaaagacac atttcaccca gggttacacc agaagccacc atataaacgg
     3541 ggagatcttt ctgtgcacag aaagcatcgc gttgtttctt taatgcatgg tataggtccg
     3601 ggtgtgtact actgtcagtg actgctgaac tgacacccgc atacacgttg acagaaaaat
     3661 ggggaagtat aaaactccgg cgtcgctggt gccaggtatc cgtatccagg ctaccgttaa
     3721 atcctttata taaatgaata gccagcgata aaccggcaaa caggtctttc agtaaatcgt
     3781 tcacctcctt tgagtgctga cggctatcgg taaccatgga cggttgctga ataaatgtaa
     3841 tcactgactc cgcttcttgt gtaaaatgtt ccgaagcctt ttgaagtctt tccagcaata
     3901 ccgtgttttc ttcagggggc aagtcagatt gaaactgtcc tgctaaccac tgatgaaacc
     3961 ggagggcggt gtcttgtaat gtattcaact tgaccaatag ctcgtcggtc caggcccggg
     4021 cttctttagt aaagcttccg gtattatcaa ggatgaattt ttttaactct atgacctcgc
     4081 cggcctgccg gtcaaaacta aaggcagcgg tcagcatctt caattggtaa gaacgccggg
     4141 aggccttcag ttctgtttgc agtaaatcag aactggcact gcgcctggta aattccacaa
     4201 tacgggggtc agtgaataag ctgttggtcc gcactttgct tctcaggatc atcccggata
     4261 aactggtaca acggctaagg gccacatata cctgcccggc agcaaacgct tcaccggcat
     4321 ctataatggc tttttcaaag gtgagaccct ggcttttatg gatggtaatg gcccaggcga
     4381 gtctcagggg aaactgggta aaggatccca gcagttcctc tttcatggac tggctggtct
     4441 tatccagtga ataccggata ttctcccact tatcccggat cacttctatt tcctccccat
     4501 cagtttcaca gcgtacataa atcttggctt cttccagccg ggttaccgtt cctatcttcc
     4561 cgttataata acgcttgcct ttatccgacg tatcattacg gataaacatc acctgggcgc
     4621 cttctttaag aaccagggtc tgttcggcgg gaaaggcatt gggtggaaaa tcattctcca
     4681 gttctgcctc atattgcacc agcggactgg tcagctgatt caattgctgg ttattaatat
     4741 tccgggcctg ttcattatgg gtggtcagga tgatatagcc gtcatctttg ctgcgtctga
     4801 acccgggttg gaaccggctt tcgaggatgg cctttccttc gggattaagt tcattgtttc
     4861 gcacctggtt cagcagactg ataaagtcag cttcgcgttg ccggtaaatc ttgtcgaact
     4921 ctatgtaaag cgggggcgct tctttcaaca ccaggctgtc aaagaagtag ggactattat
     4981 aatagggtga aagcagactc cactccggtt ctttgaccac cgggggcagc tgaaacatat
     5041 ccccgataaa taatagttgt acgccaccga atcgttcgcg gggctttcgg cgtatatgcc
     5101 ggagcaccgt gtctatcgca tccaggatat cggccctgac catactgatc tcatcaataa
     5161 tgagcaattc catttgctgg attactttct tcttgtcgcc gttaaaccgg aggcgactga
     5221 cgaggctgtg gggattgctt atttcctgtc cggccggagc aaaaccctgt ccccgggcca
     5281 cgggtacaaa cggcgcaagg ggcaattgaa aaaacgaatg gatggttacc ccacccgcat
     5341 tgatggccgc caccccggtg ggagccacga tggccatttg tttagtgcaa tgctcacgga
     5401 tatatttcag aaaagtcgtc ttgcccgtac cggctttgcc ggtaagaaat atcgtccggt
     5461 ccgtctgatt aatcagatcg gcagccagga taaaaaactg attagatata tctattttaa
     5521 atccactcat atatatgcca ggaataagtt ttgtctggct aaagccaggc aaaaaattta
     5581 gccactaaaa aacacgaata cacacgaatt aaaatcaaac aatgatccgc ttatattcca
     5641 agcgcttttt accaaaattt acaatgactc ctaatctgaa atcagaagcc ctcaggtaat
     5701 taatagtttg ttcaaaatgc ccgctgaatg tcgccacctc gtttgccttt acttctacaa
     5761 tgattttgtt atacatagta aaatcagatc tgaaccggtg tttaagttta ttggttttat
     5821 aaaaaacaaa caaatcctct tcccgaagat agggtatgtt atgttcgata aattccagtt
     5881 ccatggcgtc tttatacact acttctgaaa aaccatatcc cagggttctc cacacttcca
     5941 tacaatatcc aatgatagta taaacttctt ctttatataa aatgtctgtc ataatttgat
     6001 aaaaatgcca cgaaatc
//
LOCUS       DAOPMW010000189         6002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15221_length_6002_cov_5.081722, whole genome shotgun sequence.
ACCESSION   DAOPMW010000189 DAOPMW010000000
VERSION     DAOPMW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15221_length_6002_cov_5.081722"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1567
                     /locus_tag="PLU11_09555"
     CDS             389..1567
                     /locus_tag="PLU11_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59409.1"
                     /translation="MISQKGLFSTKIAGRSLPYNMFCIVGIVILIFNIFLSALWPFKD
                     TVTIQFFVAGIFLLLLGMVIFRYSSTFIAILLVITVYNLLIFWVSFNLGRSSGAMLYY
                     FPLMVGFLYLFLYNSNVTKTIAQILIMVVFILFTIFYTQSKSAHFFLPDPVLEKIYLL
                     NLLFSIIATIIVLIFLYRQFTSLHKNVLKDKELEHRKMLRDLDLEREKQGYALLLSLR
                     DNISQTLASSRMYLQMHPEHMEYTKKADEQVKEALDGLNDISVELSPSMLIDLGLEDG
                     LETYAALLTDKYGIPVSIDLDKKSSYIPEEERLSLYRILQQCINIIATTRSIEFLHVH
                     LIKEKKLSLHFEHDSSFKDFSYRFLDTRYRDLTTRLDYYSAVIREEKGKVEVDLDVSS
                     "
     gene            1719..2168
                     /locus_tag="PLU11_09560"
     CDS             1719..2168
                     /locus_tag="PLU11_09560"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN59410.1"
                     /translation="MKKLNLVLIAVLAIQVSFGQDLSKMEEIMNLYTSQYKFNGTVLV
                     AHKGKILLDRGYGYRIVKDSVKHDPGSVFQIGSVTKQFTAVVILKLQEENKLNVTDKL
                     SKYFPDYPKGDSITIHQLLSHTSGIFNYTNDAEFMNKEVTNPATREK"
     gene            2165..3055
                     /locus_tag="PLU11_09565"
     CDS             2165..3055
                     /locus_tag="PLU11_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN59411.1"
                     /translation="MMARFKDKPLDFSPGTKWSYSNSGYMLLGYIIEDVAKMSWEQAV
                     RKYIFNKAGMQQSGFDFAHLQHPGKSTGYFRIKADGNTPSPVVDSSVSYAAGSIFSTT
                     GDLYKWFLALQADKLINSNSKEQAFTPVKNKYGYGWGMDTLAGKRTVGHSGGIHGFVS
                     NMVFIPKDSSVVILLSNLSTPHLSAMTKHLFSVLYNLPYELPKEISAIPMTEEELKQY
                     TGIFDLNADLVVKIYVENGKLIGAPEGQEPLQLHPEKKDHFFLKEIEAKIRFNRNERG
                     EVESMTLFQNGSEITGKKRG"
     gene            3276..4382
                     /locus_tag="PLU11_09570"
     CDS             3276..4382
                     /locus_tag="PLU11_09570"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HPN59412.1"
                     /translation="MRQLLLVVLIVISSSVFSQDHSHSMGRKIVFPDIPGYKTLKTDL
                     HQHTVFSDGSVWPDIRVMEALMDGLDVISLTEHLEYQPHKTDIPHPDRNRSYELALAE
                     AKNHNLIIVRGSEITRKMPPGHNNAVFIKDANGLLIKDSVEVFKAAKAQGAFVFWNHP
                     NWTAQRKDGIARLTDMHTMLIREKLLDGIEVVNDDTYSEEALQIALDNNLTIMGTSDI
                     HKLIDWDFKVYEGGHRPVTLVFAKERTEESVKEALFERRTVVNYKDLLIGRETFLVPL
                     LQASITVKSASYTGKTDVLKVVLLNGTNSPILLNNKSGFTLHSNYDVITLQPGENTIE
                     VKTLERLKELTLNFEVLSAVYAPGKHPVVGWKVL"
     gene            complement(4460..5143)
                     /locus_tag="PLU11_09575"
     CDS             complement(4460..5143)
                     /locus_tag="PLU11_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN59413.1"
                     /translation="MYRCIAIDDEPLALNIMADYIRKTDSLHLVDICTSPQQALQRVL
                     NGEADLIFLDIEMPGLNGIEFMQLAGPQRRFILTTAYPDYALTGYEYNVVDYLLKPIS
                     YDRFLKAVHKLPLSTNLPELKRVSFFFVKSEYKLLRIEYDDVRYFESLRDYVAIHTVT
                     GQKILTLQSLSSFEKELPATQFIRIHKSYIVAVNKLELIAKNRVKIGIEELPIGDTYR
                     SNLNRLTKH"
     gene            complement(5146..>6002)
                     /locus_tag="PLU11_09580"
     CDS             complement(5146..>6002)
                     /locus_tag="PLU11_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN59414.1"
                     /translation="LLVFRRYYYAKPRWKLVLGIVLLLLSFILYRYSVEELLFPALLG
                     EGNYNPGTTFRFYFFDNVYYGSIRIFIGFVLFLLDELIRSRIQQAALKQQQQKAELRF
                     LEAQMNPHFLFNALNNIYALALDKHPETANAVLKLSDMMRYVTYERGPQASLEKELNY
                     IHTMLSIEQLRHDYKLQYQLISSPEAMACRLSPLLLIPLVENALKHGDLSDPSCPLEI
                     NLGKEGPRLSILIRNKKKAKPLISTKGGLGLPNLRQRLELSFRPEEYIFRIEENQQTF
                     TVNLQVPA"
BASE COUNT         1639 a         1216 c         1395 g         1752 t
ORIGIN      
        1 gtatccggga ttctacagtt atctggcatc cataaaactt gagcaagggg aaaagcaagc
       61 tgcaggaaag ctcttgcaac aggctcagct aaaactggaa aatctgcatt ttgccaaccc
      121 tgacttgctc caggaagaaa aggcgatgat tgatgaactt ctgcagctgt tgaagtaata
      181 gaaaggattt tttttcctga caacgcaacc aattcccggg attttacgat ctatactaac
      241 agttgttcac atgtccgata aaaactggta gtgtttttta aatcataatg gttttttagg
      301 attttttgta ttgaaaatta tctaactgga tcaaaatcag gattttttct gcaaattttc
      361 actacattga attaactggt taaaggctat gatttcccag aaaggtctgt ttagtacaaa
      421 aattgcaggc aggtccctgc cgtacaatat gttctgtatt gtaggtattg ttatactcat
      481 ttttaatatt tttctttccg cgctctggcc atttaaggat actgttacca ttcagttttt
      541 tgttgccgga atatttctcc ttttgctggg catggttatt ttcaggtatt cttctacgtt
      601 tatcgccatc ttgctggtaa ttacagtgta taatttactg atcttctggg tctcttttaa
      661 cctgggacgg tcttccggag ccatgttgta ttactttccg ctcatggtgg gttttttgta
      721 cctgtttttg tataattcca atgtgaccaa aacgattgcc cagatcctga ttatggttgt
      781 ctttattctg tttaccattt tttatactca atcaaagtca gctcatttct tcctgccgga
      841 tcctgtgctg gagaaaattt atttactgaa tcttttgttc tccattatcg ccactatcat
      901 tgtactgatt tttctgtacc ggcagtttac atcattacat aaaaatgtac tgaaagataa
      961 agaactggag catcggaaaa tgcttcgcga ccttgatctg gaacgggaga aacaagggta
     1021 tgcgctttta ctctccctcc gggataatat ttcgcaaacc ctggcgtcca gccggatgta
     1081 cctgcagatg catcctgagc atatggaata taccaaaaaa gccgatgaac aggtaaaaga
     1141 agcattggac gggttgaatg atatttcggt ggaactgagt ccttctatgt tgattgacct
     1201 tggactggaa gacggactgg aaacctatgc agcgttgctc acggataagt acggtattcc
     1261 cgtaagtatt gatctcgaca aaaaatcttc atatatacct gaagaagagc gattatcact
     1321 ttaccggatt ctgcagcaat gtatcaatat tatcgcgact acccgaagta tagaatttct
     1381 ccatgttcac ctgataaaag agaaaaaact cagcctgcat tttgaacatg attcatcctt
     1441 taaggatttc tcttatcgtt tcctcgatac ccgataccgt gacctgacta cccggctgga
     1501 ttattattcg gcggtgatac gggaggaaaa agggaaggtg gaagtggatc tggatgtttc
     1561 ttcgtagcag ggatattttc caacaggcag tgggccctaa gctgttcctg caacgggttt
     1621 tatgtaagat ttatgaataa gctgctttcc cattcaaatc ctttactttt ataatgtaac
     1681 ctcatctgtc tgacacacgt cagatgtaaa aattctgtat gaagaagcta aatctcgtcc
     1741 tgattgctgt attggcaata caggtttcat tcggccagga cctctccaaa atggaagaga
     1801 tcatgaatct ttataccagt cagtataaat ttaatggcac ggtgctggtg gcccataagg
     1861 ggaagatttt attggatagg ggctatggat accgaattgt aaaggatagt gtaaagcacg
     1921 atccgggcag tgtgtttcag atcgggtctg tgacaaagca gtttactgcc gtcgtgatcc
     1981 tgaaactgca ggaggagaat aaacttaatg tgaccgataa actcagtaag tatttcccgg
     2041 attatccgaa aggggatagc ataacgattc atcagttgct ttcgcatact tccggcatct
     2101 tcaattatac caatgatgcg gagtttatga ataaagaggt gaccaacccg gccacccggg
     2161 agaaatgatg gcccggttta aggacaagcc ccttgatttt tcgcctggaa cgaaatggag
     2221 ttattccaat tccggctata tgttactcgg gtatattatt gaagatgtgg ctaaaatgtc
     2281 ctgggagcaa gcagtgcgca aatatatttt caataaggcc ggtatgcagc agagtggatt
     2341 tgattttgcc catctgcaac atcccggtaa gtctacgggg tatttcagaa tcaaagccga
     2401 tgggaatacc ccatcaccgg ttgtggattc atcggtgtcc tatgcagcag gttccatctt
     2461 ctccaccact ggcgatttgt ataaatggtt tctggcattg caagctgata aacttataaa
     2521 tagcaattca aaagaacagg cttttacccc ggttaagaac aagtacggat atggctgggg
     2581 tatggatacc ctcgccggga aaagaactgt cggtcatagt ggtggcattc atggttttgt
     2641 cagtaatatg gtgtttattc cgaaagatag ttccgtggtg atcctgctga gtaatttaag
     2701 tacacctcat ctctcggcca tgactaagca tctgttttct gttctttata atctgcccta
     2761 tgagttgccg aaggaaatat ccgccattcc catgacggaa gaggagctga aacaatacac
     2821 cggtattttt gatttaaatg ctgacctggt agtaaagata tatgtggaga atggcaaact
     2881 catcggtgct cctgaaggac aggaaccttt acagttgcat ccggagaaaa aagatcattt
     2941 ctttctaaaa gagatagagg ctaagatccg cttcaaccgt aatgaaagag gtgaagtgga
     3001 atcgatgacc ttgtttcaaa atggcagtga aattaccggg aagaaaaggg ggtagccggc
     3061 agacggcagt ccacagtcca cagttcacag tcggcagaaa acagcccctt ttaagttgtt
     3121 tgttaagagt tttgggattt gtgttttttt tgcataattc taataattaa taattcaata
     3181 atagggattt agcaggggat agggcgcttc ctgatgttta ctttttactt ttgcattttc
     3241 actttggaaa ttctatcttt cataaaacac ctgttatgcg ccaactcctg ctggttgtat
     3301 tgattgtcat aagttcatcc gttttttctc aggaccatag tcattccatg ggccggaaga
     3361 ttgtatttcc cgatattccc gggtataaga ccctgaaaac ggacctgcat cagcacactg
     3421 ttttttcgga tgggagtgtt tggccggata tccgcgtgat ggaagccctt atggacgggc
     3481 tcgatgtgat ttcactgact gagcacctgg agtatcaacc gcataaaacg gacattccgc
     3541 atccggacag gaaccgctcc tatgaactgg ccctggcgga agcaaagaac cataacctga
     3601 tcattgtgcg cggatctgaa attacccgga aaatgcctcc cggtcataat aacgctgtgt
     3661 tcataaaaga tgccaacgga ctactgatca aggactctgt ggaagttttt aaagcagcca
     3721 aggcacaggg tgcttttgta ttctggaatc atcccaactg gacggcccag cgtaaggacg
     3781 gtattgcacg gcttactgac atgcatacta tgttgatcag agaaaagctg ctcgatggta
     3841 ttgaagtagt aaacgatgat acctactcag aagaagcttt gcagatagcg ctggataata
     3901 accttactat tatgggtacc agtgatatcc ataaactgat tgactgggat tttaaagtat
     3961 atgaaggtgg ccaccggcct gtgaccctcg tctttgctaa agaaagaacc gaagaaagtg
     4021 taaaggaagc cctctttgaa cgtcgtaccg tggtcaatta taaagatctc ctgatcggtc
     4081 gcgaaacttt cctggtgccg ctgctgcaag ccagcattac ggtgaaatcg gccagctata
     4141 ccggtaaaac ggatgtgctg aaggttgtgc tgctaaatgg cacaaacagt cccattttac
     4201 tcaataataa atccggattc accttgcata gtaattacga tgtgatcacc ctgcagcccg
     4261 gggagaatac gattgaggta aaaaccctgg agagactgaa agaactgacc ctgaattttg
     4321 aagtactgag tgccgtgtat gcgccgggga aacatccggt ggtggggtgg aaagtgttgt
     4381 agggtttaga agatttataa cagctttctt agaaggggct gttcactgac acctgacagc
     4441 tgacacctga caactgaatt taatgtttgg tcaatctgtt taaattactc cgatacgtat
     4501 ctccaattgg caattcttct atcccgattt ttacccggtt tttggcaatg agttccagtt
     4561 tgtttactgc cacgatatat gatttgtgaa tacggatgaa ttgcgtggca ggaagttctt
     4621 tttcgaagga actcaggctt tgcagcgtaa ggatcttctg accggttact gtatggatag
     4681 ctacatagtc ccttagcgat tcgaaatagc gaacatcgtc atattcaatt ctaagcaatt
     4741 tatattctga tttaacaaag aagaaagaaa cacgtttcag ctccgggaga ttcgttgata
     4801 aaggaagttt atgcaccgct ttcagaaagc gatcgtacga aatgggtttg agcaggtagt
     4861 ccaccacgtt gtattcgtag cccgttaacg cataatcagg gtatgctgtt gtgagaataa
     4921 accggcgctg aggtcctgcc agctgcataa attcaatccc gttcagtccg ggcatttcaa
     4981 tatccagaaa aatcaggtct gcttcaccat tcagtacccg ttgcagtgcc tgctgcggac
     5041 tggtgcagat atccaccagg tgaagtgaat ctgttttacg gatatagtcc gccatgatat
     5101 tcagggcgag tggttcatca tctatagcaa tacatcggta catagtcagg ctggtacttg
     5161 cagatttacc gtaaatgtct gttgattttc ttcaatacgg aatatatatt cttccgggcg
     5221 gaaactcaac tccagtcttt gcctgagatt gggtaagccc agcccgccct tcgtactgat
     5281 gaggggcttt gccttttttt tattccggat caggatgctc agcctgggac cttctttgcc
     5341 gagattaatt tccaggggac aggacgggtc agacaggtcg ccgtgtttaa gcgcattttc
     5401 aaccagcggt attagcaaca ggggagacag gcggcaggcc atggcttcag gggaagaaat
     5461 aagctgatac tgtaatttat aatcatgccg taattgttca atcgaaagca tggtatggat
     5521 atagttcagt tctttttcca ggctggcctg tggtcctctt tcgtaggtta catagcgcat
     5581 catgtcgctg agtttgagta ccgcgttggc ggtttccgga tgtttatcga gcgcgagcgc
     5641 ataaatattg ttgagagcgt tgaacaggaa atggggattc atctgtgctt ccaggaaacg
     5701 cagctccgct ttttgctgct gttgttttaa ggcggcctgt tgtatccggc tgcggatcag
     5761 ttcatcgagc aggaacagta caaagccaat aaagatccgg atggaaccgt agtagacatt
     5821 atcgaaaaaa tagaaacgaa aagttgtacc tggattataa ttcccttctc ccaacagggc
     5881 cggaaagagc agttcttcta cactatatcg gtaaaggata aatgacagca gcaggaggac
     5941 aatacccagg accagtttcc agcggggttt tgcatagtaa tagcgcctga atacgagtaa
     6001 gt
//
LOCUS       DAOPMW010000190         5997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15242_length_5997_cov_8.580108, whole genome shotgun sequence.
ACCESSION   DAOPMW010000190 DAOPMW010000000
VERSION     DAOPMW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15242_length_5997_cov_8.580108"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1260)
                     /locus_tag="PLU11_09585"
     CDS             complement(211..1260)
                     /locus_tag="PLU11_09585"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPN59415.1"
                     /translation="MPLHKTFCFPAASGKDVYLFTLRNVAGTEARITNYGGIITSFSV
                     PASQDTRNDIVLGFDKVEDYHSPAYLAQYPYFGCVVGRTANRIRNASFEIDGKPNPVT
                     ANFGKHQLHGGNEGFDKKIWDITGFGEFPSPWVEMKYHSPDGEEGYPGNLDVWIRFEL
                     TDDNELSYSFTARTDKTTAVNLTHHGYFNLNNGQGDIKDYELKIYSSQVLDQDEELCT
                     TGTVTPVKDTVFDFRSFTRIGERLETVPEFDKSYVVDKNNDPAGLALMAEARCAASGL
                     LLQVYATDPVVHFYTGKWIPEMTGKNDRCYGAFSGFCLETQIHPNAINIPHFPTMLLK
                     PGEEYKSKTVYKVIS"
     gene            complement(1301..2368)
                     /locus_tag="PLU11_09590"
     CDS             complement(1301..2368)
                     /locus_tag="PLU11_09590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPN59416.1"
                     /translation="MMQADCQEAVYALLPAGHPYKVEPLSGGLINHTFKVTDLQTGES
                     FLLQKINRQVFPDPEKIQANYQQLWQFLAEKSMPLFIPEMKFFNGSKNLFTDRTDQCW
                     RSFEFIGSGLQLNGTEVPAQAAAVAETFARFTASFHQLDSTLLHRILPGFHDVKQRFD
                     QFTGSLTGAPTTRLEKAAILISELKKRQRYAGLYQQFTADPDFKFRVMHHDAKIANVL
                     FDRQSGKVICPVDLDTVMPGYFFSDLGDMIRSMAGTTDENSTDFDRIQIRPAIYQSII
                     DGYTRGINEWLSGPERAYLHASGILLIYMQALRFMTDYLRNDSYYKINYPEQNFDRAK
                     NQLVLLKRLEEFLADNYKFTC"
     gene            complement(2449..3060)
                     /locus_tag="PLU11_09595"
     CDS             complement(2449..3060)
                     /locus_tag="PLU11_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HPN59417.1"
                     /translation="MEELSQDTRNILGLQLSTDPRWVDLAGLSLEDILTDHAYCEQKA
                     ATTCISLIQRYSSRVKLVAALAPIVTEEWGHFRLVLAELEKRKLALGKQRKDEYVNAL
                     IDFQQKGGDSEGRMLDQLLTMALIEARSCERFKRLSEGLQDPYMQKFYRRFMESEAGH
                     YTLFIELAETYIDKEKVRARWKEWLVYEASVMLSIDIRGDRIH"
     gene            3219..3488
                     /gene="rpsO"
                     /locus_tag="PLU11_09600"
     CDS             3219..3488
                     /gene="rpsO"
                     /locus_tag="PLU11_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPN59418.1"
                     /translation="MPLTKEKKATVLTTFAGSAKNTGSIEGQIALLTERISQISGHLQ
                     QNKKDFSTHRGLMKMVGKRKRLLNYLQKQNLTGYRQLIEKLGIRK"
     gene            3752..5944
                     /gene="pnp"
                     /locus_tag="PLU11_09605"
     CDS             3752..5944
                     /gene="pnp"
                     /locus_tag="PLU11_09605"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPN59419.1"
                     /translation="MLQQKIRKTFDIGGGRMVTIETGRLARQADGAVTVSMGNCMILA
                     TVCANTEPKEGQSFFPLSVDYQEKFAAAGRIPGSFFKREAKLNDYEVLTSRLIDRALR
                     PLFPEDYLCEVQVLVTLMSSDPEIMPDAMACLAASAALAVSDVPIKEIISEVRVSRVN
                     GQFVVNPTRSEMEKSDMDFLIAATDKNLMMVEGEAKECSEADLCKALEIAHDAIRIQV
                     KAQEELRDMAGAKPKRAYTKPASDEALKAKVYAFAQPKVYEVAKAALSKHERSDRFDA
                     IKVELVEFLKKDANLAEGENLPDLTKKLASVYYHDLQYDVIRDMILDERVRLDGRDLE
                     TVRPLDMEIDVLPSPHGAALFTRGETQSITTVTLGTPLDELLVESAAKSVDSKFYLHY
                     NFPPFSTGEIKMMRGQSRREVGHGNLAQRSLKQMMPSGDFPYTVRVVSDILESNGSSS
                     MATVCAGSLALMDAGIGIPKHVSGVAMGLITKGDKFAILTDILGDEDHLGDMDFKVTG
                     TRDGICGVQMDIKVDGLKMEVMQQALDQAKRGRLHILDAMYNTIPAARADVKPHAPRM
                     VKLFIDKEFIGAVIGPGGKVIQEIQRETGTTINLEEVNGRGEISIFSTSKDGVDKAVS
                     WIKGIVAVPQVGDTYDATVKSIMPFGAFVEFLPGKQGLVHISEISWKRLETLEGIVRE
                     GDMMKVKLIGTDPKSGKFKLSRKVLIPKPEQGPRDGQRPPAQDDNSAN"
BASE COUNT         1638 a         1382 c         1400 g         1577 t
ORIGIN      
        1 aagtttttag cggatcatgc ggagaaggta aaagtgctga tgcagaaata tccgtttctg
       61 gatctgaggg aagaacggga gtattttagt cgggagtaag aagtcggtag tcgggagtaa
      121 atacaccttc taaactgttg aatctaaaat catcaacagt ttcctgcttt acagagagat
      181 ttcgtactta caacttataa cttacaactt ttaacttatc actttataca ccgtcttact
      241 cttatactct tctcccggct tcagcagcat tgtcgggaaa tgcgggatat taatggcatt
      301 gggatgtatc tgtgtttcga ggcaaaaacc ggagaacgcg ccataacatc tatcgttttt
      361 cccggtcatt tcagggatcc atttaccggt ataaaagtgg acaacgggat cagtggcata
      421 gacctggagc aataatccag aggcagcaca acgggcctca gccatcagtg ctaatccggc
      481 cggatcattg tttttatcca ccacataact cttatcaaat tcgggtactg tttccagccg
      541 ttcaccaatc cgggtaaaac tccggaaatc aaaaacagta tcttttaccg gtgttacggt
      601 gccggtggta cataattcct catcctgatc cagtacctgt gaactataaa ttttgagttc
      661 ataatcctta atatctccct gtccgttatt caggttaaag tacccatggt gagtgaggtt
      721 aacggctgtc gttttatccg ttctggccgt aaaactataa ctgagttcgt tgtcatcagt
      781 cagttcaaaa cgaatccata catccaggtt gcccggatag ccttcttctc catccggact
      841 atggtatttc atttccaccc agggtgaagg gaattcaccg aagccggtta tatcccagat
      901 ttttttatca aatccttcgt ttcctccatg cagctggtgt ttaccaaaat tggctgttac
      961 gggattcggt ttaccgtcta tttcaaagct ggcattcctg atccggttcg cggtccgacc
     1021 gaccacacaa ccaaaatagg ggtattgtgc caggtaagca ggggagtgat agtcttccac
     1081 tttatcaaat cccagcacaa tatcgttccg ggtatcctgg gaagcaggga ccgaaaaaga
     1141 agtgattatt ccaccgtaat tcgtgatacg ggcttctgtt ccggctacat tacgcagggt
     1201 gaacagatat acatcttttc cagatgcagc ggggaagcaa aacgttttat gcaggggcat
     1261 actttgagtt gtgtggttcg tggttccctg acccggttgg tcagcatgta aatttatagt
     1321 tatctgcgag gaattcctcc agtcttttta ataaaaccag ctggtttttt gcccggtcaa
     1381 aattttgttc agggtaattg atcttgtaat aggaatcatt ccggagataa tcggtcataa
     1441 aacgcagggc ctgcatataa atcaggagga ttccggacgc atggagatac gctctctccg
     1501 gcccggacag ccactcatta attccccttg tatatccatc gattattgac tgataaatag
     1561 caggccggat ctgtatccgg tcaaaatcgg tggaattttc atctgtggtg ccggccattg
     1621 accggatcat atcacccagg tcggaaaaaa aataaccggg cataactgta tccagatcca
     1681 ccggacagat cacttttcct gactgtcggt caaaaagtac attggcaatt tttgcatcat
     1741 gatgcatcac acggaatttg aaatcaggat ctgccgtaaa ctgctggtat aagccggcat
     1801 atcgttgcct tttctttaat tcactgatca gaatagccgc tttttcaagg cgtgtggtgg
     1861 gagcgcctgt taacgaacca gtgaactgat caaaccgttg cttcacatca tgaaatcccg
     1921 gcaggatccg atgtagtaaa gtgctatcca gctgatgaaa ggaggcggta aaacgggcaa
     1981 atgtttccgc tactgcagca gcctgcgccg gaacttccgt accgttgagc tgcaaacctg
     2041 aaccgataaa ttcaaaggac cgccagcatt gatcagttcg atccgtaaac aggtttttac
     2101 taccgttaaa aaatttcatt tccggtataa aaagcggcat ggacttttca gccaaaaact
     2161 gccagagctg ctggtagttt gcctggatct tttccggatc cgggaatacc tgccggttta
     2221 ttttctgtaa aagaaaagat tccccggttt gcagatccgt tactttaaag gtatgattga
     2281 tcaacccgcc tgatagaggt tccaccttgt acgggtggcc ggctggtaat aaagcatata
     2341 ctgcttcctg gcaatcagct tgcatcatat actgtatgaa attaattcat cagcacatgc
     2401 aaaaacgcta caatcgtttg cggtaaataa ataaaagaaa ctggcagatc aatggatacg
     2461 atcgccccgg atatcgatgg aaagcataac ggatgcttca tagaccagcc attctttcca
     2521 tcgggccctt actttttcct tgtcgatata ggtttcggcc agttcaataa aaagggtata
     2581 atgtcccgct tcactttcca taaagcggcg ataaaatttc tgcatataag gatcctgcaa
     2641 accctcactc agccgcttga accgttcgca actgcgggct tcaatcagtg ccatggtaag
     2701 taactgatcc agcatccggc cttcactgtc gccgcctttc tgctgaaagt cgatcagggc
     2761 atttacatat tcatccttcc gttgttttcc caaggcgagt tttcgctttt ccagttcagc
     2821 cagcaccagc cggaaatgtc cccactcctc cgttacgatc ggcgcaaggg ccgcaaccag
     2881 tttaacccgg ctgctataac gctgaatcag tgaaatacag gtagttgccg ctttttgttc
     2941 acaataggca tggtcggtca gtatatcttc cagcgataat ccggccaggt caacccagcg
     3001 gggatccgtg gacaattgca gccccagaat gttacgggtg tcctgggata attcttccat
     3061 gaaggcaaaa ttaggttata gattgataat caggcaggag agcctgtaaa atgaccgtaa
     3121 aaaaaggcag ggaaagcttt tgctgttagc agaatccgtc ttacctttgc cgccttatta
     3181 caagatgcaa aatcggatga aatttaaaaa ttaaaaagat gcctttaact aaagaaaaaa
     3241 aagcgaccgt tttaaccacc tttgcgggtt ctgccaagaa caccggatcc attgaaggac
     3301 aaattgctct gctgactgag cgtatcagcc agatcagtgg tcacctccag caaaacaaaa
     3361 aagatttctc tactcaccgc ggactgatga aaatggtagg taaacgtaaa cgtttactga
     3421 attacctgca gaagcagaac ctgactggtt accgccagct gattgagaaa cttggtatca
     3481 ggaaataagg cctttccggc cccctaaatc ccccaaaggg ggacttggga agagtcttta
     3541 aacctgaagt tttctttcgt taagaaaagc aagttttata atactattcc caatgtcttc
     3601 cggatgttgg gaataactgt ttttatacat atcggttgct gacgttttaa tagccatatg
     3661 ctgttctgcc gttgcctgat tccgggtata aacagttcaa attctcctga tcaaattctt
     3721 cactgggtgc aacgcccaaa aattgaaaaa catgttacaa cagaaaattc gtaaaacatt
     3781 tgatattggc ggtggtcgta tggtgaccat tgaaacaggt cgtctggccc gccaggctga
     3841 tggagcggtt accgtatcca tgggcaattg tatgatcctg gccacagtgt gtgccaatac
     3901 tgaacccaaa gagggtcaga gctttttccc gctgtcggta gattaccagg aaaaatttgc
     3961 ggccgccggt cgtattcccg gatccttctt caaacgcgaa gctaaactga acgattatga
     4021 agtgctgacc agccgcctga ttgaccgggc gctgcgtccg ttatttcctg aagattattt
     4081 atgtgaggtt caggtactgg tgacgttgat gtccagcgat cctgaaatca tgccggatgc
     4141 aatggcttgt ctggctgcat ctgccgccct ggctgtatcc gatgtgccca tcaaggaaat
     4201 catctctgaa gtacgggtat cccgtgtcaa tggacagttt gtggtaaacc ctacccgcag
     4261 cgaaatggaa aaatccgata tggatttcct gattgccgct actgacaaga acctgatgat
     4321 ggtggaaggg gaagccaaag aatgcagcga agctgatctt tgcaaagcac tcgaaattgc
     4381 acacgacgcc atccgtatcc aggtgaaagc ccaggaagaa ctgcgtgata tggccggtgc
     4441 aaaacccaaa agagcgtata caaaacccgc ttctgacgaa gccctgaaag ctaaagtata
     4501 tgcgtttgct caaccgaaag tatatgaagt ggcaaaagct gctttgagta agcatgagcg
     4561 tagtgaccgt tttgacgcaa ttaaagtgga actggttgaa ttcctgaaaa aggacgctaa
     4621 tctggctgaa ggtgaaaacc tgccggatct gaccaagaaa ctggcctcag tttactacca
     4681 cgacctgcag tatgatgtga ttcgtgatat gattctggac gaaagagtac ggctggatgg
     4741 acgtgacctg gaaacagttc gcccgctgga tatggagatc gatgtacttc cttccccaca
     4801 cggcgcagcc ttatttacac gcggtgaaac acaatcaatt acaactgtta ctttaggtac
     4861 tcctttagat gaactattgg ttgaaagtgc cgctaaatct gtagattcca aattttacct
     4921 gcactataat ttcccgccgt tttcaaccgg tgaaatcaag atgatgcgtg gacaaagccg
     4981 tcgtgaagta gggcatggta acctggctca gcgttctttg aaacaaatga tgccatccgg
     5041 tgatttccct tacacggtac gtgtagtgag tgatatcctg gaatcaaatg gttcttcttc
     5101 catggcaacg gtttgtgcgg gttcattagc cctgatggat gccggtattg gtattcccaa
     5161 gcacgtttcc ggtgtcgcca tgggactgat taccaaagga gacaaatttg caatcctcac
     5221 tgatatcctg ggcgatgagg atcacctggg tgatatggac ttcaaagtaa ccggtacccg
     5281 ggatggtatc tgcggtgtgc agatggacat caaagtggac gggctgaaaa tggaagtcat
     5341 gcagcaggcc ctcgaccagg caaaaagagg ccgtttacat atcctcgatg ccatgtacaa
     5401 cacgatccct gccgcccgtg cagacgtgaa accacatgca ccgagaatgg tgaaactgtt
     5461 tatcgataaa gaattcatcg gtgccgttat cgggcccggt ggtaaagtga tccaggaaat
     5521 ccagcgggag accggcacta ccatcaacct cgaagaggtg aatggccgtg gtgaaatcag
     5581 catcttcagt accagtaagg atggggtgga taaagccgta tcctggatca agggtattgt
     5641 tgctgttccc caggttggtg atacctacga tgccaccgtt aaatccatca tgcctttcgg
     5701 tgcatttgtg gaattccttc ccggcaaaca aggactggta catatcagtg aaatcagctg
     5761 gaagcgtctc gaaaccctcg aaggaatcgt acgcgaaggc gatatgatga aagtgaaatt
     5821 aatcggaacg gatcctaaat ccggtaagtt caagctttcc cgtaaggtcc tgatccctaa
     5881 accggaacag ggtccccgcg acggacaaag acctccggct caggacgata attccgctaa
     5941 ctaaaatacc tttacagccc cggaaaaatc cggggctttt tttattatgg ctaatta
//
LOCUS       DAOPMW010000191         5985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15281_length_5985_cov_6.807083, whole genome shotgun sequence.
ACCESSION   DAOPMW010000191 DAOPMW010000000
VERSION     DAOPMW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15281_length_5985_cov_6.807083"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLU11_09610"
     CDS             <1..1088
                     /locus_tag="PLU11_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59420.1"
                     /translation="MESTCYLLKDENGKAAGLFSGDTLFIGDVGRPDLAQKVVADLTQ
                     DKLAGHLYDSLRNKIMTLPDDLIVYPAHGAGSACGKNMSKETTDTLGHQKATNYALRA
                     DMTKEEFIKEVTTGLVAPPAYFPLNVMLNIQGYDSIDEVLKRGQQALSPNGFDTAATE
                     TSALVLDTRDPQVFAAGFIPNSINIGIDGQFAPWVGAMIPDIKQEILLVTEEGREEEV
                     ITRLARVGYDYTIGYLKGGFDAWKAAGKEIDRIESIDAAELSRRMYHEKLTLLDVRKK
                     SEYGSEHLEEAINAPLDFINESMTMIDKTKTAYVHCAGGYRSMVFNSILRARGYNKLV
                     DVKGGFKALKESGLFNITDYVCPTTLL"
     gene            1123..1404
                     /locus_tag="PLU11_09615"
     CDS             1123..1404
                     /locus_tag="PLU11_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59421.1"
                     /translation="MANLKELVKDPSTIIVDVRSPMEFAMEHIPNAINIPVEQIPYKT
                     DEFKRFKNPVVVYCRSGARSGMALGILQQNGITNVYNGGGIGDMQYLFN"
     gene            1419..1727
                     /locus_tag="PLU11_09620"
     CDS             1419..1727
                     /locus_tag="PLU11_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59422.1"
                     /translation="MLGFLQKLFGGTPVDYKDLISRGAIIVDVRSQAEYKSGHINGSK
                     NYPLDSLRGKINDLKKLNKPVITVCLSGGRSGMAKNILKASGIEVYNGGPWTSLRSKI
                     "
     gene            1866..3035
                     /locus_tag="PLU11_09625"
     CDS             1866..3035
                     /locus_tag="PLU11_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPN59423.1"
                     /translation="MLRKFIKYFLVFLALALIAFFIFAPGIVDKQQNKVTLDVHKTPP
                     AKVSWYDSIPFIADLHCDALLWDRNNLKEHTHGHVDIPRMQKANMAFQVFTIVSKTPK
                     KMNIEHNDDKTDNIALLSFAQLRNPKDWFSVKTRALHQCAELFQTAKKSDGAFRVILN
                     QQDLAKFVDDRKKNPKLTAGMLGIEGAHCLNDDLSNLDEFYKAGVRYIGLTHFFDNAW
                     GGSAHGMKKDGLTEKGKQLVKKMDSLHVMIDLAHASPRTIDDIFASTQGPVLISHTGV
                     KGVCDNTRNLSDAHLLEIGRRNGLVGIGLWETAVCGTDAASTAKSIRYVADKIGVDKV
                     ALGSDFDGAITAHFDVTGLPLIVNALLKEGFSQEDIYKIMGGNVRDFFLRNLPVK"
     gene            3046..3921
                     /locus_tag="PLU11_09630"
     CDS             3046..3921
                     /locus_tag="PLU11_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPN59424.1"
                     /translation="MQTPRKYALPLLLLMITGLLIWWYNRTHPYTAPLPGAAEYGFQL
                     ISKTAEQYGPGGRINIRSNGMNCSQCHLDAGKNLQAIPLTRVYLQYPKYRDRSGSHES
                     LYKRIKDCFERSLQSMAPDSNSREIQAITAYLKWLSENSPADSITRDPLPYLTRAADS
                     TKGSRLYAATCKRCHGQNGEGFREADHSSFRYPPLWGPNSYSTGAGMYQVTKLARFIK
                     FNMPYDKLAGSVVLTNEEAYDLAAFINSQERGVFDISDDWPVKSTKPVDYPFGPYADS
                     FSADRHKYGPWLNLK"
     gene            3979..4542
                     /locus_tag="PLU11_09635"
     CDS             3979..4542
                     /locus_tag="PLU11_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPN59425.1"
                     /translation="MLDFLRQPWPWYVAGPLIGLMVPLLLIIGNKSFGISSSLRHICA
                     ACLPSKAPFFNYEWKKEAWNLFMVAGILLGGMIAGQWLNSPDPVQVNPVLVEELAGYG
                     IRDYQHIVPAELFNWNTLFSAKGLILMLGGGFLVGFGTRYAGGCTSGHSIMGLSTLQF
                     PSLVATVCFMAGGFIVANLVLPFILKL"
     gene            4547..5077
                     /locus_tag="PLU11_09640"
     CDS             4547..5077
                     /locus_tag="PLU11_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPN59426.1"
                     /translation="MSSNTDKEKKHHEFLTQNADFEVRSLDAVCLNESELKHPWWYNF
                     KYLIAGTFFGIILIKAEVISWFRIQEMFRLQSFHMYGVIGSAVLVGIVSVWLIRKFNI
                     KTIYGEPIEFVTRKLNKGNIIGGLLFGFGWALTGACPGPLFAQIGTGATATIITLFSA
                     IAGTWCYGMLRDRLPH"
     gene            complement(5195..5677)
                     /locus_tag="PLU11_09645"
     CDS             complement(5195..5677)
                     /locus_tag="PLU11_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPN59427.1"
                     /translation="MSENKGEIKKMMIILSKATLENVYAAFVLANGARMEGIDSEIFF
                     TFFGLEAVHKKKNANLHIATVGNPAMHIPTMVGGLPGMEALATTMMKKEMAKIDMPEV
                     PEFLEILSDSGVRMWACKLAMDMFHYKKEDLFEGVEEVITVGDFYKKSEGEGVHMLFI
                     "
     gene            complement(5699..>5985)
                     /locus_tag="PLU11_09650"
     CDS             complement(5699..>5985)
                     /locus_tag="PLU11_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPN59428.1"
                     /translation="TIHVNEEGFMTDFKEWDNTVGEALAAENEISLSPRHWEVLGYLQ
                     TEFKNEVPLSIRKIGKSGVVDIKEFYQLFPKAPLKTATKIAGIPKPASCI"
BASE COUNT         1614 a         1407 c         1425 g         1539 t
ORIGIN      
        1 ccatggaaag tacctgctat ctgctgaaag atgaaaacgg aaaagctgcc ggtctttttt
       61 ccggtgatac cttattcatc ggtgatgtgg gccgtcctga cctggcgcaa aaagtagtgg
      121 ccgatcttac ccaggataaa ttagccggtc atctctacga ttccctgcgg aataaaatca
      181 tgacgttgcc ggatgacctg atcgtttatc cggcccatgg cgcaggcagt gcctgtggta
      241 aaaacatgag taaggaaacc acagatacat tgggtcatca gaaagcaacc aattatgcat
      301 tgcgggctga tatgacaaaa gaagaattta taaaggaagt caccacggga ctggtagccc
      361 cacccgccta cttcccgttg aatgtaatgt tgaatataca gggatacgac agtattgatg
      421 aagtactgaa aagaggacaa caggcgctgt cacctaacgg atttgatacc gccgcaactg
      481 aaaccagcgc actggtactg gatacccgtg acccccaggt tttcgccgct ggatttattc
      541 ccaattccat caatattggg attgatggtc agtttgctcc ctgggtgggt gccatgatcc
      601 ctgatatcaa acaagagatc ctgctggtaa ctgaagaagg ccgggaagaa gaagtgatca
      661 ccaggctggc cagggtaggt tacgattata ccattggtta cctgaaaggc ggatttgacg
      721 cctggaaagc cgccggtaaa gaaattgacc gcatcgaatc catagatgcg gccgaattat
      781 cccgccggat gtaccatgag aaactgaccc tgctggatgt tcgtaaaaaa agtgagtacg
      841 gcagtgagca cctggaagaa gcgatcaacg ccccgctcga ctttatcaat gaaagtatga
      901 ccatgatcga taaaactaaa acagcctatg tgcattgtgc gggcggttac cgctctatgg
      961 tcttcaactc gatactcagg gccaggggat ataacaaact cgtagacgta aaaggcggat
     1021 tcaaagccct gaaagagagc gggctgttca acattaccga ttatgtatgc ccgacaactt
     1081 tactttaatc aaaccgaatt tttaaaataa aaacaaccta aaatggccaa tttaaaagaa
     1141 ctggtaaaag acccatccac catcattgtg gatgtacgca gcccgatgga atttgcaatg
     1201 gaacatattc ctaacgcgat caatattccc gttgagcaga tcccttacaa gacggacgaa
     1261 ttcaaacgtt ttaaaaatcc ggtggtagtc tattgccgca gtggtgcccg gagtggcatg
     1321 gccctgggaa tcttacagca aaacgggatc accaatgttt acaatggcgg cggaatcggc
     1381 gatatgcaat acttatttaa ttaaataaac aaaagcacat gttaggattt cttcaaaaac
     1441 tttttggcgg caccccggta gattataaag acctgatcag cagaggggcg attatcgtgg
     1501 atgtaagaag ccaggccgaa tataaaagcg ggcatatcaa tgggtcaaag aattacccgc
     1561 tcgacagtct gcgcggtaaa atcaatgacc tcaaaaagct gaacaaaccg gttatcaccg
     1621 tctgcctcag cggcggccgc agtggcatgg ctaaaaacat actgaaagct tcaggcatcg
     1681 aagtctacaa cggcggtcca tggaccagcc tgagaagtaa gatctgatga aaggcggaaa
     1741 tcaaaagtaa aaagtcaaaa gtaaaaagat ctccgaaggt ggacgtttct tccctattcg
     1801 gcctgttccc atttttactt ttgacttttt aatttttact ttccttatct tccacaaaaa
     1861 accaaatgct ccgcaagttc attaagtact tcttagtatt tctcgccctc gccctcattg
     1921 ccttcttcat cttcgcaccg gggatcgtgg ataaacaaca gaataaagtc accctggatg
     1981 tacataaaac cccgccggca aaagtcagct ggtatgattc aattcccttt attgccgacc
     2041 tgcattgtga tgccctgctc tgggaccgga acaacctgaa agagcatacg cacggacatg
     2101 tggacatccc ccgaatgcaa aaagccaata tggctttcca ggtctttacc attgtgagca
     2161 agacacctaa gaaaatgaat attgaacaca atgatgacaa gaccgataat attgccctgc
     2221 tcagttttgc ccagttacga aatccgaagg attggttcag tgttaaaaca agggccttgc
     2281 atcaatgtgc cgaattgttc cagaccgcta aaaaatcgga tggggctttt cgtgttatat
     2341 tgaatcagca agacctggcg aaattcgttg atgaccggaa aaagaacccg aagctcacag
     2401 ccggtatgtt gggtattgaa ggggcgcatt gcctgaatga tgacctcagc aacctggatg
     2461 aattttataa agccggggtg cgctatatcg gtctcaccca tttttttgat aatgcctggg
     2521 gcggatcggc tcatggcatg aaaaaagacg gattgacgga aaaagggaaa caactggtaa
     2581 aaaaaatgga cagcctgcat gtgatgatcg accttgccca tgcttcgccc agaaccattg
     2641 atgatatttt tgccagcaca caaggccctg ttttgatttc tcataccggt gtgaaaggcg
     2701 tttgtgataa tacccgcaac ctgtccgatg cccatttgct ggagatcggc cggcgcaacg
     2761 gtctcgtggg gatcggactt tgggagaccg cggtatgcgg aaccgatgcg gcttccacgg
     2821 ctaaaagtat ccgttatgta gcggataaga tcggggtgga caaagtggcg cttggttcag
     2881 atttcgatgg agccatcacc gcccattttg atgttaccgg gcttccgttg atcgtgaacg
     2941 ccttgttgaa agaaggattc agccaggaag atatttataa gatcatgggt ggtaatgtcc
     3001 gggacttctt tttacgcaac cttccggtaa aataagcagt tgcccatgca aactcccagg
     3061 aagtatgccc tgcctttgtt actactgatg atcaccggcc tgctgatctg gtggtataac
     3121 cggacacacc catacacagc accgctgccc ggtgctgctg aatatggctt ccagctcatc
     3181 agcaaaaccg ctgaacagta tggaccgggc gggcggatca atatccgctc caatggtatg
     3241 aactgcagtc aatgtcacct cgatgccggt aaaaatctcc aggctattcc gcttacccgg
     3301 gtttatcttc aatatccaaa gtaccgggac cgcagcggca gtcatgaatc cttatacaaa
     3361 agaataaaag attgttttga acgaagcctg caaagtatgg cccccgatag taacagccgg
     3421 gaaatccagg cgataacggc ctacctgaaa tggctgtctg aaaactcgcc ggctgattct
     3481 ataacccggg accctttacc ctatttaaca agagcggccg acagcacaaa aggcagccgg
     3541 ctgtatgcag caacctgcaa acgttgtcat ggacaaaatg gtgaaggctt tcgtgaagcg
     3601 gatcattcct cctttcgtta tccgccattg tgggggccga acagttacag taccggcgct
     3661 ggcatgtacc aggtaaccaa actggccagg ttcattaagt ttaatatgcc ttatgataaa
     3721 ctggcaggaa gtgtagtact gaccaatgaa gaagcttatg atctggccgc ttttatcaat
     3781 agccaggaac gcggggtgtt tgatatcagt gacgactggc ccgtaaaatc tacaaaacca
     3841 gtagattatc cttttggacc ttatgcggat agcttttcag cagaccggca taaatatggc
     3901 ccctggctaa acctgaaata agaacagttg ttatagggga gaacatttaa attcgcgttc
     3961 aactttcaaa caaaaaccat gttggatttt ctgcgccagc cctggccctg gtatgtggcc
     4021 ggaccactga tcgggttgat ggtaccacta ttgctgatca tcggaaataa atcatttggc
     4081 atctcttcct ccctccgcca tatctgtgcg gcctgccttc cctcaaaagc accatttttt
     4141 aattatgagt ggaaaaaaga agcctggaat ttattcatgg tagccggtat attactggga
     4201 ggaatgattg ccgggcagtg gctgaacagt cctgacccgg tgcaggtgaa tcctgtgctg
     4261 gtggaagaac tggccggata tgggatcagg gattatcaac atattgttcc ggctgaatta
     4321 ttcaactgga atacgttgtt ttctgcaaaa ggactgatcc tgatgctggg tggtggattc
     4381 ctggtaggct ttggcacccg atatgccggt ggatgtacca gtggtcattc cattatgggt
     4441 ttatccaccc ttcagttccc gtccctggtg gccactgttt gttttatggc gggtggtttt
     4501 atcgtcgcca acctggtact ccccttcatc cttaaactgt aatcgcatgt cgtcgaacac
     4561 cgataaagaa aaaaagcatc atgaattcct gacacagaat gccgactttg aagtaaggtc
     4621 gctggatgcc gtctgcctga atgaatccga actaaaacac ccctggtggt acaatttcaa
     4681 atacctgatt gccgggactt tctttgggat cattctgatc aaggctgaag ttatttcctg
     4741 gttccggata caggaaatgt tccggctgca aagcttccat atgtatggcg tgatcggatc
     4801 ggctgttttg gtgggtatcg tctcggtctg gctgatccgt aaattcaata ttaaaaccat
     4861 ctacggggag cccattgagt ttgtgacccg taaacttaat aaagggaata tcatcggggg
     4921 cttgttattc ggctttggct gggcattgac cggagcctgt ccgggaccgc tatttgcaca
     4981 aatcggtaca ggcgccacgg ctactatcat caccttgttc agtgccattg caggtacctg
     5041 gtgttatggt atgttgcgtg acagactgcc gcattaacaa aaaaaggccg taccggaagt
     5101 gtttcccatc cgtgaacgga cagggatacc ctcttcggta cagcctcaac tgtaaataaa
     5161 acactcactc tttatatctc aacaaacttc ggtatcagat gaagagcatg tgtacgccct
     5221 ctccttcact ctttttatag aagtcaccca cggtgatcac ttcttccacc ccttcaaaga
     5281 ggtcttcttt cttgtaatgg aacatatcca tggccagttt acaggcccac atccgtacac
     5341 cgctgtccga aagaatctcc aggaactccg gaacttccgg catatcgatc ttcgccattt
     5401 cttttttcat catagtggtg gccagtgctt ccatgcccgg taaaccaccc accatggtgg
     5461 ggatatgcat ggccgggttg cccacagtgg caatatggag gtttgcattc ttctttttgt
     5521 gtacggcttc caggccaaag aaggtaaaga agatttcaga atctatgcct tccatacggg
     5581 caccattggc cagtacaaat gctgcatata cattctcgag ggtggctttg gacaagatga
     5641 tcatcatctt tttgatttcg cctttattct cgctcataac tttcgtttta agagttgatt
     5701 aaatacagct ggcaggtttg ggtatgccgg caattttagt cgcggttttc agcggagctt
     5761 tcgggaaaag ctggtagaat tccttgatat cgaccacgcc gctttttccg atcttccgga
     5821 tgctcagggg cacttcattc ttgaattctg tttgcaaata acctaatact tcccaatgac
     5881 gggggctgag gctgatttca ttttcagcgg ccagggcttc cccaaccgtg ttatcccatt
     5941 ctttaaaatc ggtcataaaa ccttcttcgt ttacgtggat ggttt
//
LOCUS       DAOPMW010000192         5978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15306_length_5978_cov_7.444707, whole genome shotgun sequence.
ACCESSION   DAOPMW010000192 DAOPMW010000000
VERSION     DAOPMW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15306_length_5978_cov_7.444707"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLU11_09655"
     CDS             complement(<1..1093)
                     /locus_tag="PLU11_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN59429.1"
                     /translation="MQQHRAIKQKYPDAILLFRVGDFYETFGQDAVLASRILGITLTK
                     RNNGAASSAELAGFPHHSLDTYLHKLVKAGHRVAICDQLEDPKLAKGIVKRGVTDMVT
                     PGTTVNDKLLEHHSNNFLAAIHFAENEQFGLAFLDISTGEFFTAEGDREYADKLIQSF
                     KPAEVVFQRHQQKKFKEYFNNKVYTYTLDEWIFDAGYASDTLLKHFQTHSLKGFGVDE
                     MKNGLTAAGAIIHYLKDTEHPNLQHIVSLQRIDRDDFLWMDRFTIRNLELVYGSHEGN
                     HTLLGVLDNTVSPMGARLLKRWIVFPLKDIQKINERLDTVEFLIRETELRNKLTQPIK
                     QCGDIERLVSKIPMKKINPREVMQLARGLK"
     gene            1242..2951
                     /locus_tag="PLU11_09660"
     CDS             1242..2951
                     /locus_tag="PLU11_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPN59430.1"
                     /translation="MNKLITLTGLLLTGTLLNAQGLPTDTVRVDATINNSTVYFGYGA
                     ELTHETKARVDGTTKIIVISQLSTSADINSLQVSVPEDVALLSQRYSVFYPTLPPAVK
                     NRDVERLEDSILLMNKEISRLDNLVQVDQEVLNKTGSLIEGAMNNIGDKTISGEELLK
                     LVEYYTGRIEKIKTNMYNNRQLITQANVRIAEIRKRIALMTAVTPVKQRPYGQVTLQV
                     MCKKAGDIPLQLSYYTNNAGWVAVYDVRVNSKTNKVKMVYKASLTQTTGVDWKKTKLT
                     LSTGTPNFGVTAPLLTPWYLQLYVPGIYTDLERRASLSNANRNIVQSYVDNKAMAEEV
                     VVNGYGTLKLEKKLADNINPSTVQQYTSLTQGQLNTSFEIDLPYDIASDGQLNSVTIK
                     DQEIACVLKNYAVPRMDKDAYLLAEVADWQNLDLLPGDANIIMDDTYIGKSVIDPNST
                     ADTLNLSLGRDRRIAVKRSLVKEMSSLKTSGGNTKQVFTYEVIVKNNKITDVNLLLKE
                     QHPLSTIKEVEVKLEEGGEAMVNAETGVLTWKIELKPGESRKVRFSYSVKYPKDKKIV
                     NLK"
     gene            3089..4711
                     /locus_tag="PLU11_09665"
     CDS             3089..4711
                     /locus_tag="PLU11_09665"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPN59431.1"
                     /translation="MKRLLLSLFILAGSLVSGAQNLTSSNLPIIVINTNGQEILNDPK
                     ITADMGIVFNGEGARNNVTDPFNHYSGKIGIEIRGQSSQMFPMKSYSIELWDNAGASQ
                     DKSLFGLPKESDWVLYAPYTDKTLMRNFLAYTFSREMGHWASNCRYVEVVVNGDYKGI
                     YVFMEKIKRGSGRVPITKIATSDISGDAVTGGYIFSIDKEADGWYSAIYPPYGSSGQN
                     IRFSYIYPKLSSIVTAQQDYLKKYVDSFETVLKSDLFQDKQSGWRKYADEASFIDYSI
                     VNEISRNVDGYRLSSYFNKDRQSKGGRIIAGPAWDYDLAFRNANYCRGAETTGWAYQF
                     NRDCPQDFFQIPFWWYQFEKDSAYQSGFRCRWKQVREGSLSDTRIFKLIDSVVSLTAE
                     ARSRHFQRWPVLGEYIWPNPQPIPTSYTGEITALKEWLMARTSWISSAIPNAGPCYDY
                     PAAEKASIIPAFYPNPVTNHFTLDIKSKMAQSLSVQITDAMGRSLWKQTYSLQYGENY
                     YTVQTDQWAPGIYLLSYKATSGEKGTKKLLKQ"
     gene            4736..5272
                     /locus_tag="PLU11_09670"
     CDS             4736..5272
                     /locus_tag="PLU11_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPN59432.1"
                     /translation="MRKLSMAELGRKSVEEFKASEKTPIILVLENIRSAYNVGSVFRT
                     SDAFLVQSIYIIGYSAKPPHKEIKKTALGAEETVEWKYFKTTAEAITELREAGFNVYA
                     AEQAEGSFKLNAIGFDPEEKIAVVFGNEVTGVEQTTIALCDGCLEIPQLGMKHSLNIA
                     TAAGVVLWELVRSRINAS"
     gene            5366..>5978
                     /locus_tag="PLU11_09675"
     CDS             5366..>5978
                     /locus_tag="PLU11_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HPN59433.1"
                     /translation="MPFALIGFFIPAANRIQVFSKVYSYSGPYGGEPGVVNRPMSFSA
                     DSPKSFTELWLLLALIVLCMVFARSAAMAFNRYLDRHFDAKNPRTAVREIPAGVITPK
                     NAMAFTLISCVLFIACTWFINSICFYLSPVALAVVLGYSYTKRFTPLCHLILGLGLSL
                     APIGAYLAVTGEFALLPILFSIAVIFWVSGFDIIYALQDEEFDK"
BASE COUNT         1591 a         1343 c         1478 g         1566 t
ORIGIN      
        1 gttttaatcc gcgggccagt tgcatcactt cacgcggatt gattttcttc atcgggattt
       61 ttgaaaccag ccgttcaata tccccgcatt gtttgatggg ttgggtaagc ttattccgga
      121 gttcggtttc acggatcagg aattccactg tgtcgagccg ctcattgatc ttctgaatat
      181 cttttaaggg gaaaacaatc catcgtttca gcaaccgggc acccatcggg gatacggtat
      241 tatcaagcac ccccagcagg gtatgattgc cttcatggct tccatatacc agttccaggt
      301 tacggatggt aaaacgatcc atccagagaa aatcatcccg gtcgatccgt tgcagggaaa
      361 cgatatgctg aagattcgga tgctcggtat ctttcagata atggatgatg gccccggcgg
      421 ccgttaatcc gttcttcatt tcatctacac cgaatccttt cagggaatgc gtctggaaat
      481 gtttcagtaa agtatcggat gcataaccgg catcaaagat ccattcgtca agggtgtagg
      541 tatagacttt gttattgaaa tattctttaa acttcttttg ctggtggcgc tggaatacca
      601 cttccgccgg tttgaaactc tggatcagtt tatcggcata ttcgcgatct ccttctgctg
      661 taaaaaactc accggtagag atgtcgagaa aagccagacc aaattgttca ttttcggcaa
      721 agtgaatggc ggccaggaaa ttattgctat gatgttcgag cagtttatca ttgacggtgg
      781 ttccaggcgt taccatatcg gttacccctc tcttcacaat gccctttgcg agtttgggat
      841 cttccagttg gtcacagatg gccacccggt gcccggcttt aaccagttta tgaagatagg
      901 tgtccagtga gtgatgagga aaaccggcca gttcagcaga agacgcggcc ccattgtttc
      961 gtttggtcag cgtaatgccc agaatccggg aagccaatac ggcatcctgg ccaaaggttt
     1021 cgtaaaaatc cccgaccctg aataagagaa tggcatccgg atatttttgt ttaatagccc
     1081 ggtgctgctg catcattgga gtttcggaac ctgctgattt cgccatatat aagtccccgc
     1141 ccggcagtta taaagctatc cccggcaggg ggcgacaaaa ataaggaact gtttatgtaa
     1201 gaaccggcct ttccgcatct gttgaaaaaa ataaagcagt catgaacaaa cttatcaccc
     1261 tcaccgggtt gcttctaacc ggcacccttt taaacgccca gggcctcccc accgatacgg
     1321 tacgggtgga tgccactatc aataattcca ccgtttattt tggttatggg gccgaactga
     1381 cccatgaaac caaggcccgg gtagatggaa ccacaaaaat tatcgtcatc agtcagctca
     1441 gtaccagcgc ggatatcaat agtctgcagg tcagcgtgcc ggaagacgta gccctgcttt
     1501 cacaacggta tagcgtgttt tatccaaccc ttcccccggc tgtaaaaaac cgggatgtgg
     1561 aaaggctgga agacagtatc cttttaatga ataaggaaat cagccggctc gacaacctgg
     1621 tgcaggtgga ccaggaagta ctgaataaga ccggctcgtt gatcgagggt gcgatgaata
     1681 acattgggga taaaacgatt tccggggagg aattactcaa actggtggag tactatacgg
     1741 gccggatcga gaagattaaa acgaatatgt acaataaccg tcagcttatt acccaggcca
     1801 atgtccgtat cgccgagatc cgtaaacgga ttgcattgat gacggcggta accccggtca
     1861 aacaacgtcc ttatggtcag gtgacgctgc aggtgatgtg taaaaaagcg ggggatattc
     1921 cattgcagtt atcatattac accaataatg cgggttgggt agccgtatat gatgtacggg
     1981 tgaattcaaa gaccaataaa gtgaaaatgg tatacaaagc ctccctgact caaaccaccg
     2041 gggtggactg gaaaaaaact aaactcacac tgagtacggg tactcccaat tttggggtaa
     2101 ccgcgccgtt actcacacca tggtacctgc aactctatgt accgggcatt tataccgacc
     2161 tggaaagaag ggccagcctg agcaatgcga accggaatat tgtgcaatcc tatgtcgata
     2221 ataaagccat ggcggaggaa gtggtggtaa atggatatgg caccctgaaa ctggaaaaga
     2281 aactggctga caatataaat cccagtaccg tgcaacagta taccagtctg acccagggac
     2341 aactgaatac cagttttgaa attgatctgc cctatgatat tgcaagcgac ggacaactca
     2401 acagcgttac catcaaggac caggagatcg cctgtgtgct gaagaattat gcagtgcccc
     2461 gtatggataa ggacgcctac ctgttggctg aagtggccga ctggcaaaac ctggacctgt
     2521 tgccgggtga tgcgaatatt attatggatg acacctacat tggtaaatcc gtgattgatc
     2581 ccaacagcac tgcggataca ctgaatcttt cgctcggccg tgatcgccgt attgccgtga
     2641 agcgttccct ggtgaaagag atgagcagtc tgaaaaccag cggcggcaat accaaacagg
     2701 tatttaccta tgaagtgatt gtaaaaaata ataagatcac cgacgttaac ctgttactca
     2761 aagagcaaca tccgctcagt accattaaag aagtggaagt gaaactggaa gaaggagggg
     2821 aagccatggt gaatgccgaa accggtgtgc ttacctggaa aatagaactg aagcccgggg
     2881 aaagccgtaa agtcagattc agttatagtg tgaaatatcc gaaggataag aagattgtga
     2941 atctgaagta gtgagtggtg agtagtgagt agtgagtagt gagtgggcaa cagctaattg
     3001 tttggggatg ccggtacggg tttttgggtc tgtgccggca tcatttttag tctccggcca
     3061 aatggttatc tttaattttt agcactatat gaaaaggctc ctactttccc tgtttatttt
     3121 agcgggttca ttggtatccg gcgcacagaa cctgacctcc tctaatctgc ccattattgt
     3181 gatcaatacc aatggacagg aaatactcaa tgatccgaag atcacggcgg atatgggcat
     3241 tgtctttaat ggggaggggg caaggaacaa tgtaacggat ccattcaatc attatagtgg
     3301 aaaaatcggc attgagatca ggggtcagtc ttcccagatg ttccccatga aatcttacag
     3361 tattgaatta tgggataatg cgggggcttc tcaggataaa tccttattcg gtttgcctaa
     3421 agaaagtgac tgggtattat atgcacccta tacggataaa accctgatgc ggaattttct
     3481 ggcctatact ttttcccggg aaatggggca ttgggcttcc aattgccggt atgtggaagt
     3541 ggtagtgaac ggggattaca aagggatcta tgtgtttatg gaaaaaatca aacggggcag
     3601 tggccgtgta cccatcacaa aaatcgccac ctccgatatc agcggggatg cggtgaccgg
     3661 gggctatatt ttttcgatcg ataaggaagc ggatggctgg tattcggcca tatatccgcc
     3721 ctatggtagc agcggacaaa atatacggtt ctcttatatc tatcccaaat tgagttctat
     3781 cgttacggcc cagcaggatt atctaaaaaa gtatgtcgat agttttgaaa cggttctcaa
     3841 aagcgatctg tttcaggaca aacaatcggg gtggcggaaa tatgcggatg aggcttcttt
     3901 tattgactat tcaatcgtga atgaaatcag ccgcaatgtg gatggctacc ggctgagttc
     3961 gtattttaat aaagaccgtc agagtaaagg cggaaggatc atcgcaggcc ctgcctggga
     4021 ttatgacctg gcttttcgta atgcaaacta ttgccggggt gcggaaacca ccggctgggc
     4081 ttatcagttt aaccgggatt gtccgcagga tttttttcag attccttttt ggtggtatca
     4141 gtttgaaaaa gacagtgctt atcaatccgg attccgttgc cgctggaaac aggtgcggga
     4201 ggggagtctg agtgataccc gtatttttaa actgattgat tcggtggtgt ccttaaccgc
     4261 tgaagcccgc agcaggcatt ttcagcgttg gcctgttctg ggcgaatata tctggcccaa
     4321 tccacaaccc attccaacca gttatacagg ggagatcaca gcgctgaaag agtggctgat
     4381 ggcccgaacc agctggatca gttcggcgat tcccaatgca ggcccttgtt acgattaccc
     4441 ggctgccgaa aaagcctcga tcatacctgc tttttacccc aatccggtca caaatcattt
     4501 taccctggat atcaaatcaa aaatggcaca atcacttagt gtgcaaataa cagatgccat
     4561 ggggcgttcc ctgtggaaac agacctatag cctgcagtac ggggagaatt attataccgt
     4621 gcaaactgac cagtgggcgc ctggtatcta cctgctttcc tacaaagcca ccagcggcga
     4681 aaaaggaaca aaaaagctcc ttaaacaata agcccactgc ccttaacttt gcgccatgcg
     4741 aaaactgagc atggctgaac tggggcgtaa gtctgttgaa gagtttaagg cttctgaaaa
     4801 aacaccgatt attctggtgc tggagaatat ccgcagtgcc tataatgtag gcagtgtgtt
     4861 ccggacctct gacgctttcc tggttcaatc gatctatatc attggctact cggccaaacc
     4921 tccccataag gaaattaaga agaccgcatt gggcgccgaa gaaacggtgg aatggaagta
     4981 ctttaaaaca accgcagaag cgattaccga attaagggaa gccgggttta atgtgtatgc
     5041 cgccgaacag gctgagggga gttttaaact caatgctatt ggctttgatc cggaggagaa
     5101 aattgccgtg gtttttggca atgaagtgac tggtgtggag cagactacaa ttgctttgtg
     5161 tgacggatgt ctggaaatcc ctcaactagg tatgaaacac tccttaaaca tcgccaccgc
     5221 tgctggtgtg gtgctgtggg aattagttcg tagccggatc aacgcttctt agccggtact
     5281 cattactcat tattcattac tcattttcat gaccaaggta aaatcatact taagcctcat
     5341 taaattctcg cataccattt ttgcaatgcc ctttgcattg attggttttt ttattcctgc
     5401 agccaaccgg atacaggtct tttctaaagt ttattcctat agtggccctt acggtggaga
     5461 gccgggtgtc gtaaatcgtc caatgtcatt tagcgcagac agccccaaaa gttttacgga
     5521 gttgtggttg ttattagcct taattgttct ctgcatggtc ttcgcccgca gcgccgccat
     5581 ggcattcaac cgttatctcg atcgtcattt tgatgcgaag aatccccgga cagccgtccg
     5641 ggaaatacca gccggtgtaa ttacgcccaa aaatgcaatg gccttcacgc taatcagttg
     5701 cgttttattt atcgcctgta cctggtttat caattccatt tgcttttacc tgtcaccggt
     5761 ggccctggcc gtagtgctcg gctatagtta taccaaacga ttcaccccgc tttgtcacct
     5821 gatactcgga ctgggtttat ccctggcacc gatcggggct tacctggccg tcaccgggga
     5881 gtttgccctt ttacccatcc ttttttccat tgcagttata ttctgggtca gtggctttga
     5941 tattatttat gccctgcagg atgaggagtt tgataaat
//
LOCUS       DAOPMW010000193         5972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15329_length_5972_cov_7.022478, whole genome shotgun sequence.
ACCESSION   DAOPMW010000193 DAOPMW010000000
VERSION     DAOPMW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15329_length_5972_cov_7.022478"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1828
                     /locus_tag="PLU11_09680"
     CDS             179..1828
                     /locus_tag="PLU11_09680"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPN59434.1"
                     /translation="MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGLRVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIFTTQANNVTTGRIYQTVINKE
                     REGAYLGKTVQVVPHITDEIKRRMQLIGQSGDYDIVITEIGGTVGDIESLPFVEAVRQ
                     LQWELPDEDAVVVHLTLIPYLKAAKELKTKPTQHSVRMLSQEGVHPDIIVCRTERTLG
                     PDIRRKIALFCNVKQEAVIESIDASTIYEVPLLMMREGLDKIVLKKMNITGYKEAELS
                     KWKEFLDKLKYPKGKVTIGLIGKYLELQDAYKSILESFIHAGAINECQVQIVNVHSEF
                     ITDDNVVEKLAGLDGLLVAPGFGHRGVEGKIVAVKYARENKLPFFGICLGMQMAAIEF
                     ARNVLGLKDAHSTEMDPGTKEPVIDLMEEQKKVTAKGGTMRLGAYPCDIKEGTLAHRI
                     YNNTSISERHRHRWEFNNSYLEQFEKAGMMASGKNPGTGLVEIIELANHPFFIGVQYH
                     PELKSTVENPQPVFVHFIKAAKEFAEKKVEARNPLLQSEMI"
     gene            2016..3875
                     /gene="yidC"
                     /locus_tag="PLU11_09685"
     CDS             2016..3875
                     /gene="yidC"
                     /locus_tag="PLU11_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPN59435.1"
                     /translation="MNFDRNTIIGFVVLALLFFGYFYYNNQEQKRYNDKVKREKAFSD
                     SIAALNKPKTDTLAQKKDSAQADTQRVVAASGTFRDAVKGTEQLIQTSNELLNITFSS
                     KGAQVKKVELKNFKGLDSNLLRLASSDFDRLSYPVVTGPNQTLQSADFFFNKIDSAKN
                     ADGSKTYAFTLTSSDSNNAARIVHKYTVKPNDYMIDFDIEFQGADQLLTQGNLNLSWQ
                     YSAAQQETDIDFEKQNTQVGFVENEEFDYYTVGNRNSVDFNKSVSWVGVRQRFFFTIF
                     QAKKNFSSGKLEWTLPSDSSHVLIQSTANMKVQLPVTSSAAAGFSMYYGPSDYHILKK
                     YDQKFEKLVNLGQGMYAFVRPLNRFVILPIFDFFKGLTPGSMGLAIALLTLFIRLLIS
                     PLTYSSYLSGAKMKALRPEIARLKEKTAGDQQAMSMEQMKLFREAGVNPLGGCIPALL
                     QIPIFFALFSFFNSEVSLRGAEFLWANDLAAFDDPIKFGFSIPFLGSHLSLFNVTAAA
                     TSFLISWYSMSMTPDQSNPMMKYLPYIFPVFLLFFFNKLPSALTWYYTVSNAVTLILQ
                     FIIQNYIIDHDKILAKIEENRKKPKTKSKWQERMEQMQEQQKKLKDTQHKNKR"
     gene            3994..4659
                     /locus_tag="PLU11_09690"
     CDS             3994..4659
                     /locus_tag="PLU11_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPN59436.1"
                     /translation="MKKIYTISALLGILMISTDLLAQRRLTEATISYDIVINTTNETP
                     QAADLLDGATSVVYLKGSSSRSDMISSLGTQATIVDGKTGNVTVLKEYGEQKYMITMT
                     RENWKETNKKYEGITFTYVEEFKTIAGYNCQKAIGKLTDGSTFNVYFTRDLVPVNKDF
                     QYLNKNLPGLAMQYEATLGNMLVTYTVSTINFNPVQQSKFDLPKSGFRVMTYEESKGG
                     KQN"
     gene            complement(4667..5038)
                     /locus_tag="PLU11_09695"
     CDS             complement(4667..5038)
                     /locus_tag="PLU11_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59437.1"
                     /translation="MKHNRANTTWTSGLKKLGIILLFACTGMGAYATLGDGNKKTDKP
                     KTKILSGRTTSKPGSFSLKSGYNYRGNSVINTETNKRTIRLNTVVTVQKGRTTFIVPL
                     KKNVVLNKVKLDLGNRQFQRQ"
     gene            5303..>5972
                     /locus_tag="PLU11_09700"
     CDS             5303..>5972
                     /locus_tag="PLU11_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59438.1"
                     /translation="MQENPYREDSELLQELLRQFENLKNGRPHSFIEEDSFEKIIDHY
                     HGNEDLKMAMSAATHACEQYPYSAMLRIKKADILLARRQYHTALDVLEQATLLDSGDI
                     NLYILKTEVYLALDQQEKAVTLLEEAISLFEGEERMELLFELADVYDDYEDFEKVFDC
                     LKLILEDDPNNEEALYKICFWTDFTGRNEEGIRLHLKIIDEYPYNELAWFNLAAAYQG
                     LKLYE"
BASE COUNT         1718 a         1287 c         1403 g         1564 t
ORIGIN      
        1 aaatttctat tacctttaca tgacctcccg gattttttct gccgaactac ttccgatggt
       61 cagctgggtg gggcgaattt tcagaatttt tatggataaa tattgagtag ttccgggtta
      121 ccgggatgca gtattttttt tgttcctccc tttttttaat tgactaaact gacccattat
      181 ggctaaatac atctttgtta cgggaggcgt tacttcatcg ttaggaaaag gaatcatcgc
      241 ggcatcactt gccaaattac tccaggcaag gggcctgcgt gttaccatcc agaaatttga
      301 tccgtatatc aatgtggatc cgggcaccct caatccttat gaacacgggg aatgttttgt
      361 taccgaagac ggagcggaga cggatcttga tctcggtcac tacgaacgtt tcctgaatat
      421 ctttaccacc caggccaaca acgtaaccac gggccggatc taccagacgg tgatcaacaa
      481 agaaagggag ggggcttatc tgggcaaaac cgtacaggtg gtaccacata ttacggatga
      541 gataaaaaga agaatgcaac tgatcgggca aagcggcgat tatgatattg taattactga
      601 gattggcgga acagtagggg atattgagtc cttgcccttt gtagaagctg tacggcagtt
      661 acaatgggaa ttgcccgacg aagatgccgt agtggtgcac ctgaccctga tcccttacct
      721 gaaagccgca aaagaactga agaccaaacc cacgcagcac agtgtgcgga tgctgagcca
      781 ggaaggtgta cacccggata tcattgtctg ccggacagaa cggaccctcg gcccggatat
      841 ccgccgcaaa attgccctgt tctgtaatgt gaaacaggaa gcagtgatcg aatccattga
      901 tgcttctact atttatgaag tacccctgtt gatgatgcgg gaaggactcg ataagattgt
      961 gctcaaaaaa atgaatatta ccggttacaa ggaggctgaa ctgagcaagt ggaaggaatt
     1021 tctggataaa cttaaatatc caaaggggaa agtaaccatt ggcctgatcg gcaaatacct
     1081 ggaactgcag gatgcctata aatccatcct ggagtctttt atccatgccg gtgcaattaa
     1141 cgaatgccag gtgcagattg tgaatgtaca cagcgaattc atcaccgatg ataatgtagt
     1201 ggaaaaactt gccggcctcg acggtctgct ggtagcgccg ggattcggac accggggggt
     1261 ggaaggcaag atcgtggctg tgaagtatgc ccgtgaaaat aaactgcctt tctttggtat
     1321 ctgcctgggt atgcagatgg ccgctattga atttgcgcgg aatgttcttg gacttaagga
     1381 cgcgcattca actgaaatgg accctggtac caaagagcca gtgattgacc tcatggaaga
     1441 acagaagaaa gtgactgcta aaggcggaac catgcggtta ggtgcttatc catgtgatat
     1501 caaggaaggt acactggctc atcgtattta taacaataca tctatcagcg aaagacatcg
     1561 tcaccgctgg gagtttaata acagctacct cgaacagttt gaaaaggccg gcatgatggc
     1621 cagtggtaaa aaccctggca caggtctcgt ggaaataatc gaactggcaa atcatccatt
     1681 ctttatcggg gttcagtacc acccggaact gaagagcacc gtagaaaacc cgcaacccgt
     1741 atttgttcat tttattaaag cggccaagga atttgccgaa aagaaagtgg aagccaggaa
     1801 tcccttgctg cagagtgaaa tgatttagca gttaccagtt ggcagtcgcc agttagcagt
     1861 aggcagtagg cagtaggcag ttgcctgcca cctgcctact gctgcctgcc tactgctgtc
     1921 taccgcctgc ctactgttgt ctgccgcctg ccgactgtat attgcctact gccgactgat
     1981 taccctatct ttgcgacctt aaatttccct ccagaatgaa tttcgatcgt aacaccataa
     2041 tcgggtttgt cgtccttgcg ctgttgttct ttggctactt ctattataat aaccaggagc
     2101 agaaacgcta taatgataaa gtgaagcggg aaaaggcctt tagtgattcc atcgccgcgc
     2161 ttaacaaacc caaaactgat acgcttgcac agaaaaagga tagtgcccag gcggatacac
     2221 aacgggtagt ggccgcttcc ggtacattca gggatgctgt aaagggaaca gaacaactga
     2281 ttcagaccag caatgaactg ctcaatatta cgttcagcag caaaggcgct caggttaaaa
     2341 aggtggagtt aaagaacttt aagggcctgg acagtaacct gctccggctc gcgtcttctg
     2401 atttcgaccg gctgagttat cccgtagtca ccgggccgaa ccaaacgctg caaagcgccg
     2461 atttcttttt caataagatc gactccgcta aaaatgcaga tggttcaaaa acctatgcat
     2521 ttaccctcac gtcgtctgac agtaataatg ccgcccgtat tgtacacaaa tacacagtga
     2581 agcccaacga ttacatgatc gattttgata tcgaatttca aggggcggat cagttgctga
     2641 cacaagggaa cctgaatctt tcctggcagt attcagccgc tcagcaggaa accgatattg
     2701 atttcgaaaa gcaaaacacc caggtgggtt ttgtggaaaa tgaagaattt gattattaca
     2761 cggtgggcaa ccggaatagt gttgatttta acaaatcagt gagttgggta ggggtgcgcc
     2821 agcgtttttt cttcacgatc ttccaggcaa agaaaaattt cagttccggt aaactggaat
     2881 ggacgcttcc gtcagacagt tcacatgtcc ttatacaatc cactgccaat atgaaggttc
     2941 agctgcctgt aacctcatca gctgcagccg gtttcagtat gtattatggt ccttcggatt
     3001 atcatattct caaaaagtat gaccagaaat ttgaaaagct ggtgaattta ggtcagggta
     3061 tgtatgcatt tgtccgcccc ctgaatcgtt ttgtaattct cccgatcttc gatttcttta
     3121 aagggttaac accgggcagt atgggattgg ccattgcctt acttaccctt tttatccgtt
     3181 tgttgatttc cccgttaaca tattccagtt acctgagtgg tgcaaaaatg aaagccctca
     3241 ggcccgaaat tgcccggctg aaggaaaaaa cggccgggga tcagcaggcc atgagcatgg
     3301 aacagatgaa attattccgg gaagccgggg tgaatccgct gggaggttgt attccggccc
     3361 tgcttcaaat cccgatcttc tttgccctgt tcagcttctt taactcggaa gtcagtctcc
     3421 ggggtgcgga attcctctgg gccaatgacc tggccgcttt tgatgatccg attaaattcg
     3481 gattcagtat cccattcctg ggaagtcacc tgagcttatt caacgtaacg gcagcagcca
     3541 ccagcttcct gatttcctgg tacagtatga gtatgactcc ggatcaaagt aatccgatga
     3601 tgaaatacct gccttatatt ttcccggtat ttcttttatt cttcttcaat aaactgcctt
     3661 cagcccttac ctggtattat actgtttcaa atgcggtgac cctgatcctg caatttatta
     3721 tccagaatta tatcattgat cacgataaga tcctggcaaa aattgaggaa aaccggaaaa
     3781 agccaaagac caagtccaaa tggcaggaac ggatggaaca aatgcaggaa caacagaaaa
     3841 aactgaagga tacgcagcat aagaacaagc gctaagctgt taaaataacc ttaagaaaga
     3901 ccgggatttg ttgcaaaaca cccggttttt ctgtttaagc aacttattgg ctaaattggc
     3961 acgtctttta gatatactat acagttaact accatgaaaa agatatacac gataagcgca
     4021 ttactgggaa tactgatgat cagcactgac ctgttggccc agcgccggct cacggaagcc
     4081 accatttcat atgatattgt gattaatacc accaacgaaa ccccgcaggc cgctgatttg
     4141 ctcgatggtg ccaccagtgt ggtttacctg aaaggcagtt ccagccgttc agatatgatc
     4201 agttcccttg ggacacaggc cacgattgtg gacggtaaaa cagggaatgt gaccgtgttg
     4261 aaggagtacg gggaacagaa gtatatgatc accatgaccc gtgaaaactg gaaagagaca
     4321 aacaaaaagt acgaagggat cacgtttacc tatgtggagg aattcaagac cattgccggt
     4381 tataactgtc agaaagctat cggtaagttg acggatggaa gtacgttcaa tgtttatttt
     4441 acccgggatc ttgttccggt aaataaggat tttcagtatt taaacaagaa tctgcccgga
     4501 ttggctatgc agtacgaagc cacccttggc aacatgctgg tgacgtatac cgtttccacg
     4561 atcaatttca atcccgtcca gcagtcaaaa tttgacctgc ctaaatcagg attcagggta
     4621 atgacctatg aagaaagcaa agggggaaaa cagaattaga ttaaaattac tgacgctgaa
     4681 actggcggtt accgagatcc agttttactt tattgagcac tacgttcttt ttgagtggaa
     4741 caataaacgt agtccggcct ttctgtacgg taacaaccgt gttcagccgg atggttcttt
     4801 tgtttgtttc agtattgatt actgaattac ctcggtaatt atatccggat tttaaagaga
     4861 aagaaccagg cttggaagta gtgcggccgg aaagaatttt ggttttgggt ttgtcagttt
     4921 tcttatttcc gtcacccaat gtggcataag cacccatacc ggtacaggca aaaagcagga
     4981 tgattcccag ctttttaagt ccgcttgtcc atgtggtatt ggcccggtta tgcttcatta
     5041 attgcaaatg tagtgacaaa aaaaaatata aaaaaaccgt tcgtccagaa ctttagtaca
     5101 ctattaacgg ttcaggctgt aaattgttac atgcaggccg gtaaaaaaaa tacctatttt
     5161 gcacatctgg ggaaaactag gcggcgattc tactggtttt ttttacttaa cttgttgata
     5221 acaacagttt tcagggggca aagggatttt gctgtctgaa ccaggcctta aacagattca
     5281 gcacttttaa agaccggaat tcatgcaaga aaatccatac cgggaagata gtgaactact
     5341 tcaggaactg ctcagacaat ttgaaaactt aaaaaatggc cgcccgcatt cttttattga
     5401 agaagattcc tttgaaaaaa taatcgatca ttatcacggt aatgaggacc tgaaaatggc
     5461 aatgtctgcc gcaacccacg cctgtgagca atatccctat tcggcaatgt tacggatcaa
     5521 aaaagccgat attttgctgg ccagacggca atatcatacg gcgcttgatg tactggaaca
     5581 agccaccttg ttggacagcg gggatatcaa tctctatatt ctgaagacgg aagtctacct
     5641 ggcactggat cagcaggaga aagctgtaac cctgctggaa gaagcgattt cgttgttcga
     5701 gggagaggag cggatggaac tcctgtttga attggcggat gtatatgacg attacgaaga
     5761 ctttgagaaa gtatttgatt gcctgaaact aattctggag gatgatccga ataatgaaga
     5821 agcactgtat aaaatctgtt tctggacaga tttcaccgga cgtaatgaag agggaatccg
     5881 gcttcattta aaaattatag atgaatatcc ttataatgaa ctggcctggt ttaaccttgc
     5941 ggctgcttac cagggtttaa agctctatga aa
//
LOCUS       DAOPMW010000194         5916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15520_length_5916_cov_10.711312, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000194 DAOPMW010000000
VERSION     DAOPMW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15520_length_5916_cov_10.711312"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PLU11_09705"
     CDS             <1..1245
                     /locus_tag="PLU11_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPN59439.1"
                     /translation="TEMNYWPVESGNLSECAEPLLRMMQDITDQGTQVAREHYGARGW
                     VLHQNTDLWRVAAPMDGPTWGTFTVGGAWLCTQLWEHYAYTKDKNYLKEVYPLLEGSV
                     QFFMDFLVPHPNGKWLVTNPSTSPENFPDGGGNKPYFDEVTAGFREGTTICAGSSIDM
                     QILYDLFGYFTEAAAVLHISDSLTQQVKAAREKLVPPQIGKDGSLQEWADDWKSLEEN
                     HRHFSHMYGLYPGKVLYEKRTPALIEAYKKVLEERGDASTGFSRAWKMALWARLGDGN
                     RANKIFKGYLQEQTCTSMFALCGRSLQVDGNFGVTAAVTEMLLQSQDGWIRLLPALPV
                     EWSEGSFTGVCTRGAFELDYSWKDGKVTQLNIKSKAGETCRIEWKPGMKITCNGKTVP
                     FKKENNGVVAFQTSKDSVYRIQ"
     gene            1271..3496
                     /locus_tag="PLU11_09710"
     CDS             1271..3496
                     /locus_tag="PLU11_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN59440.1"
                     /translation="MIRFSSFLFIVFVLIAGNSIAQTKKLTLEDIYSKNRYGSRGVGP
                     LRWMKDNKSYSTLEYNAAAGGSDIVRYEVETGTKTVLISAAQLVPASGSKPLSMDDYE
                     WSGDNSKLLLFTNTKKVWRYNTRGDYWVLDLKTGKLTQLGKSLPVSSLMFAKFSPDGS
                     RVGYVSQQNIYVEDISTGVITQLTKDGGGDIINGTFDWVYEEELDDRDGFRWSPDGTT
                     IAYWQSDTKGVGTFYLINNTDSNYSKPLPFPYPKVGTANSAVKVGVVAATGGPSKWFD
                     VPGDPRNNYLARMDFIPGSGEVMIQQLNRLQNSNKVWIGNTANMQLKLLFTESDSAWV
                     DLHDDIVWLDNNKYFTWTSEKDGWVHLYKVSRDGKEMKPITKGSFDVVNINCIDPAGG
                     YVYYIASPENFTQRYLYRSRLDGKGDAERVSPAGMAGQHSYQISADAKYAIHSFENAV
                     TPRRISLINLGTHKEIKLMADNQELKSRISELGLRPKEFFKVDIGPVVLDGWMIKPIS
                     FDPQKKYPVIFHVYGEPAGSTVQDNWGTGDNMWHQYLANELGCIVISVDNRGTKVPRG
                     RDFRKCIYRQIGLLAADDQAAAAKKIMQTYTFIDSAHVGMWGWSGGGQMTLHGLFRHG
                     NVFKTGIAVSFVALQTLYDNVYQERYMGLPEDNKYGYKEGSPLTHAGKLHGKLMIIHG
                     TGDDNVHYQNFEMMVNELIKNNKLFSMMSYPNRDHGIWQGVNTTLHLRRTMEQFWKLN
                     L"
     gene            3512..5089
                     /locus_tag="PLU11_09715"
     CDS             3512..5089
                     /locus_tag="PLU11_09715"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPN59441.1"
                     /translation="MAVYPSIFNDVIGPVMRGPSSSHCAASLRIARICRDLMDGEIRH
                     ILIEFDPNGSLATTHKSQGSDMGLFGGFLGWEADDERLPASERYLAEAGITVEFVVRD
                     MGADHPNTYQLTLSSEKEVRRLTAISTGGGMIEVISIEGASVSMAGDYYETIIGCTEP
                     DRIVRYLEATVLHDSITLHQGEHPFIILKAQSFPDAVILSELQAMESVLFIKKIKPVM
                     PVMARKDLEVPFISCNEMLAYNNGKDKSLWELAIDYEMARGNISAEEVMEKMRAIVRI
                     MGHAIETGLKGTEYKDRILGSQSPRFREKMKAGALVEGDAGNLMILYVSAIMEVKSSM
                     GVIVAAPTAGSCGALPGALFGVAHALKLSEDELVKAMLSAGLIGVFIAAHATFAAEVG
                     GCMAETGSGGGMAAAAIVQLKGGTLEQSLAAASMALQNSLGIICDPIGNRVEAPCLGR
                     NVMAAGNAISSANMALADYQHLIPLDEVIATMKAVGDQIHHTLRCTNLGGLSITDTAK
                     KIEARLEEQPVRLYKNC"
     gene            5174..>5916
                     /gene="hflX"
                     /locus_tag="PLU11_09720"
     CDS             5174..>5916
                     /gene="hflX"
                     /locus_tag="PLU11_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPN59442.1"
                     /translation="MIDKKNKIQQEEKAVLVAVIQKDQTETQVVEYLDELAFLAETAG
                     AITVKRFTQKLAHPDSRHFVGKGKLEEIKNYLKGKDIGVVIFDDELSGAQINNIEKEL
                     EVKTIDRSDLILDIFARRAKTAQARAQVELAQYQYILPRLRGMWKHLERLGGGIGTRG
                     PGETEIETDRRIVRDKITLLKKRLAEIDKQAFTQRKDRGEFIRVALVGYTNVGKSTLM
                     NLLSKRDVFAENKLFATLDTTTSKVVYENT"
BASE COUNT         1581 a         1283 c         1607 g         1445 t
ORIGIN      
        1 acggaaatga attactggcc ggtggaaagc ggcaatcttt ctgaatgcgc cgaaccgctt
       61 ttacgcatga tgcaggatat taccgatcag gggacacagg tggcccgcga acattacgga
      121 gcccgcggat gggtcctcca ccagaacacc gatctctggc gggtggccgc tcccatggat
      181 ggtcctacct ggggcacttt caccgtaggc ggtgcctggc tctgcacgca gttatgggaa
      241 cattatgcgt atacaaaaga taaaaactat ttaaaggagg tttacccttt gctcgaagga
      301 tccgtgcagt tcttcatgga cttcctggtg ccccatccca atggaaaatg gctggtcacc
      361 aatccttcca cgtcaccgga gaactttccg gatggaggag gcaacaaacc ttattttgat
      421 gaagtaacgg ccggattcag ggaaggcact accatctgtg ccggttcttc gattgacatg
      481 cagattctct atgatctctt tggttatttt acagaggcag ctgctgtttt acacatttca
      541 gattcattga cgcagcaggt gaaagcggcc cgtgaaaaac tggtgccgcc acagatcggt
      601 aaagatggct cgctgcagga atgggcggat gactggaaat cactggaaga aaaccatcga
      661 catttttctc atatgtatgg attgtatccc ggtaaagtat tgtatgaaaa aagaacaccg
      721 gccctgatcg aagcgtataa aaaagtgctg gaagaaagag gggatgcttc caccggtttc
      781 tccagggcct ggaaaatggc gctctgggca agactgggtg atggaaaccg cgccaataaa
      841 attttcaaag gctacctgca ggaacaaacc tgcacgtcga tgtttgcctt atgcggccgg
      901 tccttacaag tggatggtaa ttttggggta accgctgcgg ttacggagat gctgctgcaa
      961 tcacaggacg gatggatccg tttgttgccg gctttacctg ttgaatggag tgaaggcagc
     1021 ttcaccgggg tttgtacccg tggcgctttc gaactggact atagctggaa ggacggcaag
     1081 gtcacacaac tgaacattaa atcaaaagcc ggggaaacct gccgcattga gtggaaaccg
     1141 ggcatgaaga tcacctgcaa cggaaaaact gtaccgttca aaaaggaaaa caacggggta
     1201 gtggcttttc agaccagcaa ggatagtgtt tatcggattc aataatcgta cattcattta
     1261 aattaaaaat atgattcgct tttcttcctt tctgttcatc gtgtttgtcc tgattgcagg
     1321 caacagtata gcacaaacga aaaaactgac tttggaagac atctattcca aaaacaggta
     1381 tggttcccgg ggggtaggcc ctttacgctg gatgaaggat aataaaagtt attccaccct
     1441 ggagtacaat gcagctgccg gcggatcgga tatagtgcgg tatgaggtgg aaaccggaac
     1501 aaagacagtc ctgatcagcg ctgcacaact ggtgccggcc tccggaagta aacccttgtc
     1561 catggatgat tatgagtggt cgggggataa cagtaaactt ttactattta ccaatacaaa
     1621 aaaggtatgg cgttacaata cccggggcga ttactgggtg ctcgatctga aaaccggtaa
     1681 actgacacaa ttaggaaaaa gcctgcctgt ttcctccctg atgtttgcca aattttctcc
     1741 ggatggcagc cgcgtaggct atgtgagtca gcagaatatt tatgtggaag atatcagcac
     1801 cggggtgatc acccaactga ccaaagatgg cggtggcgat attatcaacg gaacctttga
     1861 ctgggtctat gaagaagaac tggatgaccg cgatggtttc cgctggagcc cggatggaac
     1921 taccattgct tattggcaat cagatactaa aggagtaggc actttttacc tgatcaataa
     1981 cacagattcc aattattcca aaccccttcc atttccttat cccaaagtgg gtacggctaa
     2041 ttcagcagtg aaagtggggg tagtagcggc caccggtggc ccgagcaaat ggtttgatgt
     2101 gcccggcgat ccgcggaata attacctggc gcggatggat tttattcccg gttccggtga
     2161 agtgatgatc cagcaactta accgtctgca gaacagtaat aaggtatgga tcggcaatac
     2221 agcgaatatg caattgaaat tattattcac tgaaagtgac agcgcctggg tggatctgca
     2281 cgatgatatt gtgtggctcg ataacaacaa gtattttacc tggaccagcg aaaaagatgg
     2341 ctgggtgcat ttatataaag tatcccgtga cgggaaggag atgaagccga tcaccaaagg
     2401 gagttttgat gtggtgaata tcaactgcat tgatccggcc ggtgggtatg tgtattatat
     2461 cgcttcaccg gagaacttca cccagcgtta tctctaccgc agccggctgg atggaaaggg
     2521 cgatgcggaa cgggtgtcgc cagcgggaat ggccggacaa cacagttacc agatctccgc
     2581 cgatgcgaaa tatgccattc acagttttga aaatgccgtg acgcccagac gtatttcact
     2641 gatcaacctg ggcacccata aagaaatcaa actaatggcc gataaccagg aactgaaaag
     2701 ccgtatcagt gaactcggtc ttcgtcccaa ggaatttttt aaagtggata taggtcctgt
     2761 ggtactggat ggctggatga tcaaaccaat cagctttgat ccgcaaaaaa aatatcccgt
     2821 catttttcat gtatatggag aacccgcagg ctcgaccgtt caggataatt ggggtaccgg
     2881 cgacaatatg tggcatcagt acctggccaa tgaacttggt tgtattgtaa ttagtgtaga
     2941 caacagaggt accaaagtgc cgaggggtcg tgatttccgt aaatgtattt accggcagat
     3001 cggtttactc gctgccgatg accaggcagc tgctgccaag aagatcatgc agacttatac
     3061 ctttatcgat tccgcccatg tgggtatgtg gggctggagt ggtggcggac aaatgaccct
     3121 gcatggattg ttccggcatg gcaatgtgtt caaaaccggt attgctgttt cctttgtggc
     3181 cctgcaaacc ttgtatgata atgtatatca ggaacgctat atgggtttac cggaggacaa
     3241 taaatacggg tataaagaag gttcacccct gacccatgcg ggtaaattac atggaaaact
     3301 gatgatcatt catggcactg gtgatgataa tgtgcattat caaaattttg aaatgatggt
     3361 gaatgaactg ataaagaata acaagttgtt cagtatgatg tcgtacccca accgggacca
     3421 tggcatctgg cagggcgtga ataccacctt gcacctgcgg agaacgatgg aacagttctg
     3481 gaaactgaat ttataatctt caatcaatcg tatggcggtc tatcccagta tattcaatga
     3541 tgtgatcggt cctgtgatgc ggggcccttc cagttcgcat tgtgcggcct ctttacgcat
     3601 tgcccgtata tgccgcgacc tgatggatgg ggagatccgt catatcctga ttgaatttga
     3661 tccaaacgga tcattggcca ccacgcataa aagccagggt tcggatatgg gattgtttgg
     3721 cggtttcctt ggttgggaag ccgatgatga acggctgcca gcatcagaac gttacctggc
     3781 ggaagccgga attaccgtag agtttgtggt tcgcgatatg ggggcggatc atcccaatac
     3841 atatcagctc acgctcagta gtgaaaagga agtacgacga ctgacggcta tttcaactgg
     3901 cggggggatg attgaagtga tcagtattga aggagcatcg gtatccatgg ccggtgatta
     3961 ttatgaaacg attatcggtt gtacggagcc ggatcgtata gtccgctacc tcgaagcaac
     4021 tgtgttgcat gattccatca cgttacatca gggagagcat ccttttatta tactcaaagc
     4081 gcagtcattc ccggacgctg taatcttaag tgaattgcag gcgatggagt ccgttctttt
     4141 cattaagaaa ataaaaccgg tgatgccggt catggcccgc aaagacctgg aagttccgtt
     4201 tatttcctgt aatgagatgc tggcgtataa taacgggaaa gataaatcac tctgggaact
     4261 agccattgat tatgaaatgg cccggggcaa tatttcagcg gaggaagtga tggagaaaat
     4321 gcgggctatt gtacgaatca tgggtcatgc cattgaaacg ggcttgaagg gcacggagta
     4381 caaagaccgg atactgggca gtcagtcgcc ccggttccgc gagaaaatga aagccggtgc
     4441 gttggtagaa ggcgatgccg gcaatctaat gatcctatat gtctccgcca ttatggaagt
     4501 aaagagttcg atgggcgtga ttgtggcagc gcctacggcc ggcagttgcg gggccttgcc
     4561 tggcgctttg tttggggtgg cccatgcgtt gaaattatcg gaagacgagt tagtaaaagc
     4621 catgttatct gccggactga ttggggtgtt cattgccgct catgccacat ttgctgcgga
     4681 agtgggcggt tgtatggccg agactggttc gggtggggga atggccgccg ccgccatcgt
     4741 acaactcaaa ggaggtacac tggagcaatc cctggctgct gcctcaatgg ccctgcagaa
     4801 ttcattgggt attatctgcg atcccattgg caaccgggtg gaagcaccct gcctgggaag
     4861 aaatgtgatg gcggccggga atgccatttc cagtgccaat atggctttag ctgattacca
     4921 gcacctgatc ccgttggatg aagtgattgc aacaatgaag gcggttggcg atcagatcca
     4981 tcatacactt cgttgtacca atctgggtgg attgtccatt accgatacgg ctaaaaaaat
     5041 tgaagcccgg ttggaagagc aaccagtccg tttgtataaa aactgctgat aatcccgtaa
     5101 aacaccggaa ctgcccttct cgtgctttat ttttaccttt gcttttccct cgttcgccgg
     5161 gggaattttt attatgattg acaagaagaa taagatacag caggaagaga aagccgtact
     5221 ggtggcggtg attcaaaaag atcagaccga gacccaggtg gttgaatacc tggatgaatt
     5281 ggctttcctt gccgagacgg ccggggccat aacggtaaag cggtttaccc agaaactggc
     5341 gcatcccgac agccggcatt ttgtcggcaa gggaaagctc gaggaaatca aaaactattt
     5401 aaagggcaag gatatcggcg tggtcatttt tgatgatgaa ctcagtggtg ctcagatcaa
     5461 taatattgaa aaggagctgg aggtaaagac catcgaccgc agtgacctga tcctcgacat
     5521 ttttgcccgc cgggccaaga ctgcccaggc aagggcgcag gtggaactcg cccagtacca
     5581 gtatatctta cctcgtctgc gtgggatgtg gaaacacctt gagcgtcttg gtggtggtat
     5641 tggaacgagg gggccgggtg aaacggaaat tgaaactgac cgtcgtatag taagggataa
     5701 gatcacttta ttaaagaagc ggctggcaga gatcgacaaa caggcgttta cgcaacgcaa
     5761 ggatcgcggt gaatttatcc gcgtagcgct cgtcggttat accaatgtgg gcaagagtac
     5821 gctgatgaac ctgctcagca agcgggatgt gtttgccgag aataaattat ttgccaccct
     5881 cgatacgacg accagtaaag tggtatatga gaatac
//
LOCUS       DAOPMW010000195         5890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15620_length_5890_cov_12.936932, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000195 DAOPMW010000000
VERSION     DAOPMW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15620_length_5890_cov_12.936932"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..701)
                     /locus_tag="PLU11_09725"
     CDS             complement(102..701)
                     /locus_tag="PLU11_09725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPN59443.1"
                     /translation="MAFTLAPLPYAHDALEPHIDTLTMQIHHGKHHQAYVDNLNKAVA
                     GTPNESKTLEELVAVAGSISPAVRNNGGGHWNHTFFWESLAPNAGGAPTGKLAEAIDA
                     AFGSFDAFKEKFANAGMTRFGSGWTWLLVKDGKLEISSTPNQDNPLMDVADVKGTPIL
                     GVDVWEHAYYLKYQNRRAEYLGAFWNVVNWSTVAERFGK"
     gene            complement(821..1258)
                     /locus_tag="PLU11_09730"
     CDS             complement(821..1258)
                     /locus_tag="PLU11_09730"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPN59444.1"
                     /translation="MTDEYFMMQALKEARIAYDEEEIPIGAVVVMNHTIVARGHNMTE
                     RLNDPTAHAEMIALTAAFNSLGSKYLPDATLFVTVEPCSMCAGAAYWSKIGRVVYGAE
                     DEKNGYKKTAGANWPFHPKTELVHGVLKEECAQLMKDFFKSRR"
     gene            1312..2685
                     /locus_tag="PLU11_09735"
     CDS             1312..2685
                     /locus_tag="PLU11_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPN59445.1"
                     /translation="MTNISRLQDMRHFFDSGATRTYAYRKQQLLAFKACLKKHEQDIY
                     AALFTDLKKVPEESWFTENGLLMQEINHTLSHLDEWMQPVRTRTNLVNLPSSSRLYPS
                     PLGVVLIIGPWNFPLQLLLIPAIGALAAGNCVVLKPSEYAPATATLIEKMIKETFPEN
                     LVSVVPGDGAEVVPAMMNNFRFDHVFYTGSIPVGKAIYQLAAKDLVPVTLELGGKDPC
                     VVEADANLEVAARRITLGKFSNAGQMCVSPDYLLVHASVKDRLMELMKKYIIKFFGDQ
                     PETTYGYNKIINLKNFDRLTRYLADGKIITGGQYNRDSLYIAPTMLEEVNPDAPVMQE
                     EIFGPILPVFTFNEPAEALAMIRRHPNPLSFYIFTSSSKKEKDWIEQVQFGSGCVNNT
                     AMQFTNHHLPFGGVGQSGIGAYHGRDSFDVFTHYKSVMKTPVWFDPALKYPPLKGKMK
                     LLKFFIK"
     gene            2691..3371
                     /locus_tag="PLU11_09740"
     CDS             2691..3371
                     /locus_tag="PLU11_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPN59446.1"
                     /translation="MKKLKRFGLVLLFLIASFVVYVEIVNRNSVNMTYRQKILKAIYP
                     AFMWITRVTGANSEKLANKDKVPPVPFSTLQVKLNNGDSLDFSTLQGRKILLVNTASD
                     CGYTHQYEDLQALSVAYGQKLLVIGFPANDFKEQEKGTDEEIGAFCKKNYGVTFPLAQ
                     KAVVIKSAAQHPVFQWLSDSAKNGWNSKAPSWNFSKYLVNEKGILTHYFDPSISPASE
                     EVKAAINE"
     gene            3383..3898
                     /locus_tag="PLU11_09745"
     CDS             3383..3898
                     /locus_tag="PLU11_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59447.1"
                     /translation="MFTKNNIEKYFDGEKDESRVFLFIGITGLLISLFFLLYGTNRFY
                     MGAAIPLAVIGLLLAMVGYTIYKRSDNDRIRNVYAYDMNPAELKEKEIPRMKTVMRNF
                     VIYRYTELFLLALGIGLYIYFIRDFNNDFWRGFGLALAVMALIALTADFFAEQRGKKY
                     LKGLEEYCARN"
     gene            complement(4044..>4388)
                     /locus_tag="PLU11_09750"
     CDS             complement(4044..>4388)
                     /locus_tag="PLU11_09750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPN59448.1"
                     /translation="SLIGRTPLSKISPKKTWEGTIGGMILAALVIGWLAAHYDKVFVF
                     DQSFTWWTLLAAVAAIIGTIGDLLESKLKRMAGVKDSGSLMPGHGGFLDRFDSLLLAT
                     PFVWGFVQLFLV"
     assembly_gap    4389..4488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4489..4787)
                     /locus_tag="PLU11_09755"
     CDS             complement(<4489..4787)
                     /locus_tag="PLU11_09755"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPN59449.1"
                     /translation="MAFNWKTFNTRALTALVFVAVMMTGLLLNHWSFFLLFSVIHFGC
                     WWEYFKLTEKNYQTSFHIYTKLGFMLLGYGLMLWFCGDVFQLGSYSLRDNFSMPVS"
     gene            complement(4805..5755)
                     /locus_tag="PLU11_09760"
     CDS             complement(4805..5755)
                     /locus_tag="PLU11_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPN59450.1"
                     /translation="MKGLFKNKSAGNQLLLMVCIALVGVFIIGSLLGTIVLSAITGIQ
                     STELLDFNKLDFNKPGMITYIRGLQVFQFLGLFLVPSLLCAWFFSNNSKKYLGLRQPH
                     GESYWIIGMLVMILAIPFVQWLGEMNRGIQFPGAVAQWMKEKENGANKTVMALLSRRT
                     IQDLVLNIICIAGLAAVGEELLFRGVAQRLFIRLFKRVWPGIIITAFLFSAMHLQFYG
                     FFPRFVLGILLGVIYWYSGSLWTAILAHFVYDALLITLAYFNPRLINEENTVKMSSIA
                     LTAAFSFILVGLAVNWMIKHSKTRYQEVFEEDIAPVDNRQ"
BASE COUNT         1583 a         1420 c         1319 g         1468 t
ORIGIN      
        1 ggggttgaac taaaaaaaag ccggccctaa cagagccagc ttttcaatat tgaaacaagc
       61 actccggcga accttcgtac ttcgtacctc gtacttcgta cttatttccc gaatcgttcc
      121 gctaccgtac tccagttcac cacattccag aatgcaccca ggtattccgc tcttctgttc
      181 tggtacttca ggtaatacgc atgttcccat acatccacac ccaggattgg ggtgcctttt
      241 acgtcggcca catccatcag gggattatcc tggttggggg tggaactgat ttccagcttg
      301 ccgtctttta ccagcaacca ggtccagcca ctgccaaaac gggtcatacc ggcattggcg
      361 aatttttctt tgaacgcatc aaatgaaccg aaagccgcat cgatcgcttc cgccagttta
      421 cctgtcgggg caccaccggc attcggagcc aggctttccc agaaaaaggt atgattccaa
      481 tgaccaccac cgttgttgcg tacagccggg gaaatcgagc ctgcaacagc caccagttct
      541 tccagtgttt tactctcatt tggagtgccg gccacggctt tattcaggtt atctacatag
      601 gcctggtggt gttttccatg atggatctgc atggtcaggg tatcgatatg cggttccagt
      661 gcatcatgtg catagggaag aggtgctaat gtaaatgcca taataatata gatttaattg
      721 tgattgattc aatgttgttt aaacgaataa caaaattaag ccctgcaggt ttaccaggaa
      781 ggggtgtgta ttaattgttt ctgatttcgg aaaccgggca ttaccgcctg cttttgaaga
      841 aatccttcat cagctgggca cattcctctt ttaaaacccc atgcaccagt tcggtctttg
      901 gatgaaaggg ccagttggca ccggccgttt ttttataccc gttcttttcg tcctctgcac
      961 cgtacaccac ccggccaatc ttgctccagt aagccgcacc ggcacacatg gaacagggct
     1021 ccacggttac aaaaagggtg gcatcgggca ggtatttgct gccgagggaa ttgaaggcgg
     1081 cggtcagggc aatcatttcg gcgtgtgcgg tggggtcatt cagccgctcg gtcatattat
     1141 gtccccgggc cacaatggta tgattcatca ccactaccgc cccgatcggt atttcttctt
     1201 catcataggc gatccgcgct tccttgagcg cctgcatcat aaaatattca tcggtcatgt
     1261 tataagctaa gctatctcta tttggtataa cttgggataa aattagcacg aatgaccaat
     1321 atatcccgct tgcaggatat gcgccacttt tttgacagcg gagccacacg cacgtatgca
     1381 taccggaaac aacaattact ggcctttaaa gcctgtttaa aaaaacatga acaggatatt
     1441 tatgcagccc tttttaccga cctgaaaaaa gtaccggaag aaagctggtt caccgaaaac
     1501 ggactgctca tgcaggagat caatcacact ttgtcgcacc tggatgaatg gatgcagccg
     1561 gtccgcaccc gtaccaacct ggtaaacctg ccttcttcca gccgcctcta tccctcgccc
     1621 ctcggcgtgg tgttgattat tggtccctgg aattttccct tgcagttgtt attgataccc
     1681 gcaattggtg cccttgcggc cggaaattgt gtggtattga aacccagtga atatgccccg
     1741 gccaccgcca cgctaattga aaaaatgata aaagaaacat ttccggagaa tctggtcagc
     1801 gtggtgcctg gtgatggagc tgaagtagta ccggccatga tgaataactt ccggtttgac
     1861 catgtgtttt ataccggcag tattcccgta ggaaaagcga tctatcagct cgcggcgaaa
     1921 gacctggtac ccgtaacgct cgaactgggt ggtaaggatc cctgtgtggt ggaagccgat
     1981 gcgaatctcg aagtggccgc ccgtcggatc accttgggaa aattttccaa tgccggacaa
     2041 atgtgcgtat ctcccgatta cctcctggta catgccagtg taaaggaccg gctgatggaa
     2101 ctgatgaaga agtatattat aaaattcttt ggcgatcaac cggaaaccac ctacggttac
     2161 aacaagatca tcaacctgaa aaatttcgac cggctgaccc gctacctcgc tgatggaaaa
     2221 attataaccg gtggacagta taaccgtgat agtttatata tcgcgcccac aatgcttgaa
     2281 gaagtgaatc cggatgcccc cgttatgcag gaggaaatct tcggtcccat cctgccggtc
     2341 tttacattta atgaacctgc tgaagcactg gccatgatcc gacgccaccc caatccgctt
     2401 tccttttata tttttaccag tagcagcaaa aaggaaaaag actggattga acaggtgcag
     2461 tttggctccg gttgcgtaaa taataccgcc atgcagttta ccaatcacca tttgccgttt
     2521 ggcggggttg gacaaagcgg tattggcgct tatcacggaa gggatagttt tgatgtattt
     2581 actcattata agtccgtgat gaaaacaccg gtctggtttg acccggccct gaaatatccg
     2641 ccattgaaag ggaaaatgaa attgcttaaa ttctttatta aataagccgg atgaaaaaac
     2701 taaaacggtt cgggcttgtt ctcttattcc tcattgcctc gtttgtggta tatgtggaaa
     2761 ttgtaaaccg taattcagtc aacatgactt atcgccagaa aatactcaaa gctatctacc
     2821 cggcctttat gtggatcacc cgggtgaccg gtgccaattc cgaaaagctt gctaataaag
     2881 ataaagtccc tcccgtgccg ttctcaacct tacaggtaaa gctgaataac ggggacagcc
     2941 tcgacttctc cacgctgcag ggacggaaaa tcctcctggt taataccgcc agtgattgtg
     3001 gctacacgca tcagtacgaa gacctgcagg ccctttctgt tgcgtatgga caaaagctgc
     3061 tggtcattgg ttttcctgcc aatgatttta aagaacagga aaaaggaacc gatgaggaga
     3121 tcggggcttt ttgcaaaaag aattacgggg taacttttcc tttagcacaa aaagccgtgg
     3181 tgataaaatc ggccgcccag catccggtct ttcaatggct cagcgattcc gcaaaaaacg
     3241 gatggaacag caaggccccc agctggaatt tttccaaata cctggtgaat gagaaaggga
     3301 tattaaccca ttatttcgat ccttctatat caccagcaag cgaggaagtg aaagcagcca
     3361 tcaacgaata aatccaacga caatgttcac caaaaacaat attgaaaaat acttcgacgg
     3421 ggaaaaagac gaaagccggg tctttctgtt cattggtata accgggcttc tcatctcctt
     3481 gttctttctc ctgtatggaa caaaccgttt ttatatgggg gccgctatac cattggccgt
     3541 aatcggcttg ttgctcgcca tggtgggtta tacaatctat aaaagaagtg ataatgaccg
     3601 catccggaac gtatatgctt atgacatgaa tcccgctgaa ctgaaggaga aagaaattcc
     3661 ccgcatgaaa accgtgatgc gcaattttgt catttaccgc tataccgaac ttttcctcct
     3721 tgcattgggt attggcctct atatttattt tatccgcgac ttcaacaacg atttctggcg
     3781 cggcttcggg ttggccctgg ccgtcatggc gctgatcgca ttaactgctg atttttttgc
     3841 agaacaaagg gggaagaaat atttgaaagg gttggaagag tactgtgctc gtaattaatt
     3901 ttcccgcaga ttacgcagat tttaaacgca gattttcgca gattctttct acagtaaagt
     3961 cattatagaa aagccagcgt cttccatcac tgtatttatc tgcgttaatc tgcggttata
     4021 tctgcgttaa tctgcgggga aaattaaaca agaaacaact gcacaaaccc ccacacaaac
     4081 ggcgtcgcca gtagcaaaga atcaaaccgg tccaggaaac caccatggcc tggcatcagt
     4141 gaaccgctgt ctttaacgcc cgccatacgt ttcagcttgg actccagcag gtcgccaatc
     4201 gtaccaatga ttgcggccac ggctgccagc agggtccacc aggtaaagga ctgatcaaaa
     4261 acaaaaactt tatcataatg agcagccagc cagccaatga ccagggcggc cagtatcatt
     4321 ccgccgatcg ttccctccca ggttttttta ggcgatattt tagatagtgg agtacgccct
     4381 atcagcgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga taccggcatg
     4501 gagaaattgt cccggagcga atagctgccg agctgaaata catccccgca gaaccagagc
     4561 atcagtccgt acccgagcaa cataaagccg agtttggtat agatgtgaaa gctggtctgg
     4621 tagttttttt ccgtgagttt gaagtattcc caccagcagc caaaatggat caccgaaaat
     4681 aaaaggaaaa aactccagtg gttcagcagc agcccggtca tcatgacggc tacaaataca
     4741 agcgcggtta gtgctctcgt attaaatgtc ttccagttaa atgccatatt ctaaagttaa
     4801 ttcattactg ccggttgtcc accggagcaa tatcttcttc aaatacttcc tggtaacggg
     4861 ttttggaatg tttgatcatc cagtttacgg ccagtcccac caggatgaaa ctgaaggctg
     4921 cggtcaaagc aatcgaagac atcttcaccg tattctcttc attgatcagc cggggattaa
     4981 agtacgccag ggtgatcaac agggcatcgt atacaaagtg agcgagaata gcggtccata
     5041 aactgccact gtaccaatag atcacaccca gcagaatacc caatacaaaa cgggggaaga
     5101 agccataaaa ctgcaggtgc atggcagaaa aaagaaaagc ggttatgatg atacccggcc
     5161 agacccgttt aaataatcgg ataaacaaac gctgggccac accccggaat aacaattctt
     5221 cgccaaccgc tgccagtccg gcaatgcaga tgatattcaa taccaggtcc tgaatggtcc
     5281 ggcgggagag cagggccatc acggttttgt tagcgccgtt ttctttttct ttcatccatt
     5341 gcgccaccgc cccgggaaac tgaatgcccc ggttcatctc ccccagccac tgtacaaaag
     5401 ggatggccag tatcatgacc agcatcccga tgatccagta cgattcgcca tgtggctgac
     5461 gaagacccag gtattttttg ctgttgttgc tgaagaacca ggcacaaagt aaagaaggca
     5521 ccaggaagag accgaggaac tggaaaacct gcagtccccg gatatacgta atcatccccg
     5581 gtttattaaa atcgagttta ttaaagtcaa gcagttcggt ggattgaata ccggtaattg
     5641 cagacaatac tatggtaccc agaagggagc caattataaa cacacccacc agggcgatgc
     5701 agaccatcag aagtaattga tttccagctg atttattctt aaataatccc ttcatgctac
     5761 tactctggcg ttttgattgt aattttgcag gcttaacctt taagtccttc gggcaaaaat
     5821 aggtaaatac caacggaatg gtaaaaattg gcaacataca acttcctgaa ttcccgctgc
     5881 ttctggctcc
//
LOCUS       DAOPMW010000196         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15627_length_5888_cov_8.254757, whole genome shotgun sequence.
ACCESSION   DAOPMW010000196 DAOPMW010000000
VERSION     DAOPMW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15627_length_5888_cov_8.254757"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLU11_09765"
     CDS             <1..844
                     /locus_tag="PLU11_09765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPN59451.1"
                     /translation="VAGGTESMSLVPTAGWKTVPAYSIAKEEPDYYLSMGLTAEAVAE
                     DYSVSREAQDEFSYHSHQKAIAAIQAGHFKSGILPVTVDEIYLDAKGKKQKRNYIVDT
                     DEGPRADTSVEALAKLKPAFAAGGCVTAGNSSQTSDGAAFVIVMSERMINELGLKPIA
                     RLVSSAVAGVHPRIMGIGPVAAIPKALKQAGLNLADIDLIELNEAFASQSIAVIREAG
                     LDPAKVNINGGAIALGHPLGCTGCKLTIQNLNDLKRLNKKYGMISACVGGGQGIAGII
                     ENIS"
     gene            1017..1805
                     /locus_tag="PLU11_09770"
     CDS             1017..1805
                     /locus_tag="PLU11_09770"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HPN59452.1"
                     /translation="MKQRLFLLPALILFSCLQLTAQDQPEGTETKEPAEKNTIPMNMI
                     KVNLTSLPLKQYALQYERVLSRKFSFALGVRVMPESTLPFKQAILDAVGDDPDTRDVL
                     ETFQMSNLAITPEFRFYLSKRGYGRGFYLAPFYRYASYKTSQLEFDYDDGGGGTETIN
                     MTGKLTSHTGGLLLGAQWPLGKRLVLDWWILGPHYGTGNGLFNGISSQTMTPAEQDDL
                     RTELEDLDIPLTNKKVVVTANGASLELDGPWGGIRAGFSLGIRF"
     gene            complement(1907..2986)
                     /locus_tag="PLU11_09775"
     CDS             complement(1907..2986)
                     /locus_tag="PLU11_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPN59453.1"
                     /translation="MKRFIACLLIMLAACGVRENTKPLDSDSWTIGPFVKTDSLNPIL
                     LPDTSQVFTCPVSQQKLAWESRNVLNPAAVVKEGKVYLLYRAQDPMMTSRIGLAISDD
                     GLHFTKQPAPVFYPDKDSMLALEWKGGVEDPRIVEDANGRYILTYTSYDGKTARLCLA
                     SSTDLFHWQKHGPVLGTGKYKDTWSKSGAIVVKQAGEKMIAEKINGKYWMYFGDTDLF
                     MAYSDDLIHWSPCENEETHQLIKVLTARPGRFDSRLVEPGPFALLKEEGILLIYNGSN
                     AANFNDTLLPKYTYSAGQALFDKSNPFKLIDRTDGYFIHPDKSYERTGEVNEVCFVEG
                     LVRFKNKWFLYYGTADSRIAVAVGE"
     gene            3194..4864
                     /locus_tag="PLU11_09780"
     CDS             3194..4864
                     /locus_tag="PLU11_09780"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HPN59454.1"
                     /translation="MDRRAFLKARRLNRAATDDIQQPFRTQSGISAYTGAWTEQEVIH
                     LLKRTMFGAKPSDINYFRSMSMNQAVDELLNPTAPQPNPPVKEYATSTTPGVTPDGNI
                     AQGTTWINDINSDGTVQSQRRGSYKKWWTGVLVNQDRSIREKLIMFLVDHFGNEASEV
                     GNANWNYKQHSLIRQYALGNYKELVREISKDVAMLRYLNGYLNNKNAPDENYARELME
                     LFTLGKGPGSQYTENDVKEAAKVLTGWQVNGTTYTSVFNLNRHTTTNKTFSAFFNNTV
                     ITGRNTATAGDLELADLLNMIFAQQEVAKFLLRKMYRWFVYYSIDAATETNVIEPLAE
                     IFRSNNYELKPVLSALFKSEHFYDLLNRGCMIKTPSDIVIGSMREMNVAFKPETDWDT
                     NYGLWNTFNSWMVNMGQNLHDPPNVSGMPAYYQEPSFHEIWINSDSLPKRNQFTDTMI
                     NSGYARNSFRVQFNCVAFAQSLSNPGNPNDLIDEAIRVFFRNELSAQSKSQIKTQILL
                     TGQQWDYYWTNAWTAYVSNPTTANFNTVNTRLKSLFQYLFNLSEYQLA"
     gene            4891..>5888
                     /locus_tag="PLU11_09785"
     CDS             4891..>5888
                     /locus_tag="PLU11_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPN59455.1"
                     /translation="MKRKDFLLNALPAAALLPEVINGYSVKAFDQQSPLVQALMRGQT
                     VTDHVLVIVQLSGGNDGLNMVIPVADYSKYYNARTNIAIPENRVLPIAGVSGTGLHPS
                     MTGLQTLFTEGKAKLVQAVGYPQPNFSHFRATDIWMSASNSNQEVYSGWAGRYLNYEF
                     PDFPTGYPSTTMPDPLAIQIGSTTTLTTQGPTANMGMSITNPTNFYNLVNGTTDPVPS
                     TPAGKELKYIRQVNQQTQKYSTVIKNAANSITQQGTYPTGNSLADQLKIVARLIAGGL
                     KTRVYMVSFGGFDTHSVQVGATDTTTGNHATLLQRVSDAIKAFQDDLKFQNVEDRVV"
BASE COUNT         1637 a         1379 c         1376 g         1496 t
ORIGIN      
        1 tgtggcgggc ggtacggaaa gcatgagcct cgttcctacc gctggttgga aaaccgttcc
       61 tgcttattct attgctaaag aagaaccgga ctattatctc agtatgggac ttaccgcgga
      121 agccgtggca gaggactatt ctgtaagtcg tgaagcgcag gatgaattct cctatcattc
      181 ccaccagaaa gcaatcgccg ccattcaggc cggacatttt aaatccggta ttctgccggt
      241 aaccgtggat gaaatttacc tggatgcgaa aggaaaaaaa caaaaaagaa attatatcgt
      301 ggatacggat gaaggtccgc gtgcggatac atcagtggaa gcactagcaa aattaaagcc
      361 tgcctttgca gccggcggtt gtgtaacggc aggtaactcc tcccagacat cagacggggc
      421 tgcttttgtg attgtgatga gtgaacggat gatcaatgaa ctcggactaa aacccattgc
      481 ccggctggta agttccgctg tagcaggggt acatccgcgt attatgggta tcggtcccgt
      541 ggccgccatt ccgaaagcgc tgaaacaagc cggacttaac ctggccgata tcgatctcat
      601 tgaactgaat gaagcttttg cttcccagtc catcgccgta atccgcgaag ccggattgga
      661 tcccgccaaa gtcaatatca acggaggagc cattgcattg ggacatccgc tgggatgtac
      721 cggatgcaaa ctcacgattc aaaacctgaa tgaccttaaa agactgaata aaaagtatgg
      781 aatgatcagc gcctgtgtag gtggcggaca agggatcgcc gggatcatcg agaatatcag
      841 ttaacatata cagatttatt accgtttgaa atacggaata accagtagta ccgggaacgt
      901 ttcagtaaaa tgaagcattc ccggttttat ttgaactaat ttcattctta cccgttaaaa
      961 cacgctccgg gatctcaatc attttccagt aattacactt tactaaaaac atcaccatga
     1021 aacaaagact tttcttactg ccagcactga ttcttttttc ctgcctgcag ttaaccgctc
     1081 aggaccaacc ggaagggaca gaaactaaag aaccggcaga gaagaatacg ataccaatga
     1141 atatgatcaa agtgaacctg acttcacttc cgttaaaaca gtatgcgctg caatacgaac
     1201 gggtactcag ccgcaagttc tcatttgccc tcggcgtacg ggtaatgccc gaatccacgc
     1261 tcccttttaa acaggcgatc ctggatgctg tcggtgatga tcccgataca agggatgtat
     1321 tggaaacctt tcaaatgagt aacctggcca tcacaccgga atttcgtttt tacctgagca
     1381 aaagagggta tggacgcgga ttttacctgg cgccttttta ccggtatgcc agttataaaa
     1441 caagtcagct ggaatttgat tatgatgatg gtggtggtgg taccgaaacc atcaatatga
     1501 ccggaaaact gaccagccat accggtggtt tattattggg ggctcaatgg ccattgggta
     1561 aacgactggt acttgactgg tggatcttag gtccgcatta cggtacgggg aatggtttat
     1621 tcaacgggat ttcatcccag acgatgaccc ccgccgaaca agatgatctg cgcaccgaac
     1681 tggaagacct tgatattcca ttgacgaaca aaaaagtggt ggttactgct aatggtgcca
     1741 gcctggaact ggatggcccc tggggtggca tcagggcagg attttcattg gggattcggt
     1801 tctgatcata gctggtgaaa acaccagcta tgaactaata gtttgtgtta tcactgtgta
     1861 ttatagttgg tgatatcaac gagtagtgtt ggtgataaca ccaacactac tcacccaccg
     1921 ccaccgcaat tcttgaatcc gccgtgccgt aatacaaaaa ccatttgttc ttaaacctga
     1981 ccagcccttc cacgaaacag acttcattca cttcaccggt acgttcataa cttttatccg
     2041 gatggatgaa atagccatcc gttctgtcaa tgagtttgaa aggatttgat ttatcaaaca
     2101 acgcctgtcc ggcactatag gtatacttcg gcagtaatgt gtcattgaaa ttggccgcat
     2161 tactgccgtt atatatcagc aggatgcctt cctcttttaa aagggcaaag gggccaggtt
     2221 ctaccagccg gctgtcaaac cttccgggcc gggcagtcag cactttgatc agctgatggg
     2281 tttcttcatt ttcacaaggg ctccaatgaa tgagatcatc ggaataggcc atgaaaagat
     2341 cagtatcccc aaaatacatc cagtatttac cattgatttt ttcagcaatc attttttcac
     2401 cggcttgttt taccacaatt gctcccgatt tggaccaggt atccttatac tttccggtgc
     2461 ccagcacagg accatgtttt tgccagtgaa aaagatcggt ggaactggcc aggcaaagcc
     2521 gggctgtttt gccatcatag gaagtatagg taagaatgta ccgcccgttg gcatcttcca
     2581 ctatccgtgg gtcttccacg ccgcctttcc attccagggc taacattgaa tccttgtccg
     2641 gataaaacac cggcgcaggt tgtttggtaa aatgtaatcc atcgtcactg atcgccaacc
     2701 cgatcctgga tgtcatcatc ggatcctggg ccctgtaaag gagatatact tttccttctt
     2761 tgacaacggc cgctgggttt aatacattcc tggattccca ggccagtttt tgctgactga
     2821 ccgggcaggt gaatacctgg ctggtatcgg gtagtaatat cggattcagg ctatctgttt
     2881 ttacaaaagg accaatggtc cagctgtcag aatccaaagg cttagtgttc tcccgaacgc
     2941 cgcaggcagc gagcatgatc agcaggcagg caatgaatcg tttcataatc aatgaggatg
     3001 aacaggaaag ataggttaat tgaccaatta ccccaatccg ggtcctgttt gaagccagac
     3061 tcagtctgat tcgtgctggt taaacagata ttcgtgcatt acaatttttt ttaagataat
     3121 agcggacagg cttaaacctt tttgtatttt actcatctga aataactctt tcccgggatc
     3181 aaatcaatca aatatggaca gacgtgcgtt tttaaaggcc cgccgcctga atagggcagc
     3241 aacggatgat atccaacagc catttcgtac tcaatcgggt atttcggcat ataccggtgc
     3301 ctggacggag caggaagtca ttcacctgct taaaaggacc atgttcgggg ccaagccctc
     3361 cgatataaac tatttcaggt ccatgagcat gaaccaggca gtggatgaat tactcaatcc
     3421 cacagcgcct caacccaatc ccccggtaaa agaatatgcc acctccacca ctccgggagt
     3481 aacaccggat ggtaatatcg cacagggaac cacctggatc aatgatatta atagcgatgg
     3541 tacggtgcag tcacagcgac gcggttccta taaaaaatgg tggaccggtg tattggtcaa
     3601 ccaggaccgc agcatccggg aaaaactgat catgttcctg gtggatcatt ttggcaatga
     3661 agccagtgaa gtggggaatg ccaactggaa ttacaagcaa cacagcctga tccggcaata
     3721 tgcactgggc aattataagg agttggtcag ggagatcagc aaagacgtgg ccatgctgcg
     3781 ttatctgaat ggctatctca acaataaaaa tgccccggat gaaaattatg cccgggagct
     3841 gatggaactg tttaccctgg gcaagggtcc cggatctcag tacacggaaa acgatgtaaa
     3901 agaagcggca aaagtattaa ccggatggca ggtgaatggc accacttaca catctgtctt
     3961 taatctcaac cggcatacaa ctactaataa aacgttttcc gcttttttca ataatacggt
     4021 aattaccgga aggaataccg ccactgccgg ggatcttgaa ctggccgacc tgctgaatat
     4081 gattttcgcc caacaggaag tggcgaaatt cctgctgcgg aaaatgtacc gttggtttgt
     4141 ttattattcg attgatgcgg ccacggaaac caatgtgatt gaaccattgg ctgaaatatt
     4201 ccgctctaat aattatgaat taaaaccggt cttgtccgcc ttgttcaaaa gcgagcattt
     4261 ttatgattta ctcaaccggg gctgtatgat taaaacacct tcggatattg tgatcggttc
     4321 gatgcgggaa atgaatgtgg ctttcaaacc ggaaacggat tgggatacga attacgggtt
     4381 atggaatacg ttcaattcat ggatggtcaa tatgggacaa aacctgcacg atccgcccaa
     4441 tgtatccggg atgccggcct attaccagga accttccttt catgaaatct ggatcaattc
     4501 cgactcattg ccgaaacgca accagtttac tgatacgatg atcaacagcg gttatgcgcg
     4561 gaacagtttc cgggtgcagt ttaactgtgt ggcttttgca caatctttgt ccaatcccgg
     4621 caatccgaac gacctgatcg acgaagccat ccgcgtcttt ttcagaaatg agttatcggc
     4681 ccagagcaaa agccagatca aaacccagat cctgctcacc gggcagcaat gggattatta
     4741 ctggaccaat gcctggaccg catatgtgtc caacccgacc accgccaatt ttaatacagt
     4801 gaatacaaga ttgaaatccc tgtttcagta tttatttaat ttatcagaat accagctggc
     4861 atagtttcat ataattctta atcagatcag atgaaaagaa aagatttttt actcaacgcc
     4921 ttacctgccg cggcactttt accggaagtg atcaacgggt attccgtaaa agccttcgac
     4981 cagcaatcgc cactggtgca ggcgctgatg cgtggacaaa cggtgacaga tcatgtgctg
     5041 gtgattgtac aattgtccgg tgggaatgat ggattaaata tggtaatccc tgtagcggat
     5101 tattcaaaat attataatgc aagaaccaat atcgctatcc ccgaaaaccg ggtattaccg
     5161 attgccggtg taagtggtac cggactgcat ccatccatga ccggtttgca aaccttattt
     5221 actgaaggca aggccaaact ggtgcaggct gtgggatatc cgcagcccaa tttttcacat
     5281 ttccgggcaa cggatatctg gatgagtgct tcgaacagca accaggaagt atatagtggc
     5341 tgggccggtc gttatctcaa ctatgagttt ccggatttcc caacgggata tccgagtacc
     5401 acgatgcctg atccgcttgc tattcagatt ggttccacca ccacactcac cacccaggga
     5461 ccaacggcca atatgggaat gagtattacc aatccgacta atttttataa cctggtgaat
     5521 ggaacgactg atcctgtgcc atccactccg gctggtaagg aattaaaata tatccggcag
     5581 gtcaatcagc agacacagaa atattcaact gtcataaaga atgctgccaa ttccattacc
     5641 cagcagggta cgtaccccac aggcaattca ctggccgatc agttaaaaat cgtggccagg
     5701 cttattgccg gtggattgaa gacccgggta tatatggtca gcttcggcgg gtttgataca
     5761 cactctgttc aggtgggagc taccgatacc accacgggta accatgccac cttacttcaa
     5821 cgggtatccg atgccatcaa agctttccag gatgatctta aatttcagaa tgtggaagac
     5881 agggtggt
//
LOCUS       DAOPMW010000197         5876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15661_length_5876_cov_8.406803, whole genome shotgun sequence.
ACCESSION   DAOPMW010000197 DAOPMW010000000
VERSION     DAOPMW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15661_length_5876_cov_8.406803"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLU11_09790"
     CDS             complement(<1..366)
                     /locus_tag="PLU11_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59456.1"
                     /translation="MAKLKGILKIEGTLDELTFYKTQDGHLVKTKGGVSADRIANDPN
                     FQRTRENGSEFGSSATAGKVLRNAVRNLMMNAADNRVTSRLTQIMTQIKNYDVASPRG
                     ERTVADGIADPAALALLKDF"
     gene            complement(592..771)
                     /locus_tag="PLU11_09795"
     CDS             complement(592..771)
                     /locus_tag="PLU11_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59457.1"
                     /translation="MQAHVLAKLINSNNLVLLLLKVIFNLLDEVSSIPVASACDYLNV
                     LRINAQSIHYFNYTF"
     gene            1440..2042
                     /locus_tag="PLU11_09800"
     CDS             1440..2042
                     /locus_tag="PLU11_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN59458.1"
                     /translation="MKSSLLILLGFILLSAGTEPQRYKKLFNGKDLAGWTKHGTENWY
                     VENGELICESGPDKKYGYLSTDKAYDNFILDLQFKLEANGNSGVFFRSGIEGTKISGW
                     QVEVAPPDHHTGGIYESYGRGWLIQPKPEDEKILKADDWNQLKIQAKDDEITTWLNGK
                     QIVFFKDEKIGQGKGFIALQIHDGGGIKVRWKNIRIKELK"
     gene            2094..3458
                     /locus_tag="PLU11_09805"
     CDS             2094..3458
                     /locus_tag="PLU11_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59459.1"
                     /translation="MSNNESNRRKFIRSLALSTAAVAAGTRALGNENKSAHFTIQQKS
                     RFTANDNINIALIGSGGMGVQDTITALQVPGVKLIAVCDLYDGRLQEAKTRWGKDIIT
                     TRSYKEILSRKDIDAVIIATPDHWHQQISIDALKAGKHVYCEKPMVHSILEGPAVIKA
                     QKESGKVFQVGSQGVSSLGNEKAKELLKSGAIGELNYAEGFWARHSPTGAWQYPIPAD
                     ASPANVDWETYVSNTVKRPFDATRFFRWRNYKDYGTGMSGDLFVHLFSSLHFITNSVG
                     PSKVYAAGGLRYWKDGREVPDVLLGTFDYHQHQAHPAFNLSLRCNFVDGTSGTTYLKL
                     VGSEGSMDITWDSVTLKRNKSFASDDPFFLEQMKKAGTPVTGRKRILPPEEYTFEAEK
                     GYQGGPYDHFVNFFTAIRNGGNVTEDAVFGYRAAAPALLCNDSYNQDTAILWDPEKMQ
                     LVKK"
     gene            3469..4230
                     /locus_tag="PLU11_09810"
     CDS             3469..4230
                     /locus_tag="PLU11_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN59460.1"
                     /translation="MQIKSLKIMAGLILMTGIVSCNNQAAGDKSTTDTTGNAGGDKTE
                     AAPSAWVAIFDGVSQKGWHGYNKQPGELKNWMIEDSSLVCLGAAADAHGGDIITDKTY
                     TDFELTWDWKVTKGANSGVMYHVVEDTAYQAPYETGPEYQVIDDIGFPDKLEEWQKAG
                     ADYAMHNSNDRKKLKPAGEWNSSKIIFNKGHVEHWLNGEKIVDFQAWDAEWTKKKEEG
                     KWKDYPGYGTAKTGHIALQDHGNKVYYRNIMIREL"
     gene            4990..5700
                     /locus_tag="PLU11_09815"
     CDS             4990..5700
                     /locus_tag="PLU11_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59461.1"
                     /translation="MDTKVKGHTAAFLKRQAKNLKKTKGISYLSALDQVALNAGFTNW
                     NNFVNLLNATTADSRSNQSGRKEPSRLSLTQNQQASDINPYKKLLIAALNELLNRNLI
                     SLTAPNDSVDEKGHVFLELFGYTSVVMWDDIGFEELRISVWWKYDHSKHPQANLTGNA
                     KESFSCASPLANRFHYKNFVGITASGWLERKVGKYLQGKGNRGIFDIYTRKGEKTELE
                     KISTPTPNGFGIEGPVHM"
BASE COUNT         1847 a         1139 c         1366 g         1524 t
ORIGIN      
        1 aaaatctttc aacaaagcca atgcagcagg atcagcaata ccatcggcaa cggtacgttc
       61 accacgagga gaagccacat cgtaattttt gatttgcgtc atgatttgcg ttaaacgact
      121 ggtaacacga ttgtcagcag cgttcatcat caaattacga actgcattgc gaagaacttt
      181 accggcagtt gcagagcttc caaattcaga accgttttca cgggtacgct gaaagtttgg
      241 atcatttgca atacggtcag cagatacacc acctttggtt ttcaccaggt gaccatcttg
      301 tgttttgtag aaggttaact catccagagt tccttcaatt tttagaatac cttttaattt
      361 tgccattgct ttaaattttt atgagttagt aaaaccgttt cttttttagc tgttttatca
      421 gcatttgaaa acaaaaatag aagcagtatt taggctatgc aaaagttgaa agtcatccgt
      481 tccgtttctt ccgcattatt ccaacaaaca cactttcacc gcctatgaaa atgatttata
      541 tttgcagtat cactttcata tttgaagtat atcatcggta tggatagtgt attaaaaagt
      601 ataattgaaa taatgaatag actgtgcatt tatccgaagg acattcaggt aatcacaggc
      661 agaagcgaca ggtatggaag aaacctcatc aagaagatta aagatcactt taagaagcag
      721 cagcaccagg ttgttactgt tgatgagttt tgccagtaca tgggcttgca accggaaaca
      781 gttgcaaagc aacttaggta agtagaaatc ttatctttgc ttttgtctat gataaggcac
      841 agcacaccct tgcatgaaca aagatttttc ttttctttgt gtagcgtttt cccttgctta
      901 atcagttgtt gttggctatt tgttgcccaa tcttcggaaa gccctgatat ataagggttc
      961 actatttttt gtatcgggaa gtaactcgta atgccgcaac tattaaaagg ccgcaaaaat
     1021 accatatact ttgattcgga gatattgaag ggggagagga aaagaaaaca aatagtctgg
     1081 cctcatcctt cctttccctg caatttatac ctttaccgca caccggtata gcaacaagag
     1141 accatgagga ataatacaaa aattaagaag ctgttggaag ataacgatct gaattttaca
     1201 atggagcatg aagaaattca gttaatggtt atcgacaaga ctacaaagag tagctatttg
     1261 gcgaatggta caaatattac agaagtgctc aacgacgcca tcaaatggtc aaatgcattt
     1321 aagaagcaat caaaaccggc agtttgaaga tcggctatga ttcgccggaa ccagccgggt
     1381 tttctttatc tggaagtaag gattgattga aattccaccc tttccagtat cttagggata
     1441 tgaaaagcag ccttttaata ttactgggtt ttattctttt atccgcgggt acagaaccgc
     1501 agcggtataa aaaactgttt aatggcaagg acctggcagg atggaccaaa cacggtaccg
     1561 aaaattggta tgtggagaat ggtgaactga tctgcgagag tggccctgat aaaaagtacg
     1621 gatacctttc tacggataaa gcctacgata attttatact ggatcttcag tttaaattgg
     1681 aagcaaacgg aaacagcggt gtgtttttcc ggtctggtat cgagggaaca aaaatcagcg
     1741 gctggcaggt ggaagtggca ccgcccgatc atcatacggg gggtatttat gagtcgtatg
     1801 gaagaggatg gctgattcaa cccaaaccgg aagatgagaa aatactgaaa gcggatgact
     1861 ggaatcagct aaagatccag gcgaaagacg atgaaataac cacctggctc aatgggaaac
     1921 agattgtctt cttcaaagat gaaaaaatag gacaggggaa aggctttatt gcccttcaga
     1981 ttcatgacgg gggcggtatt aaagtccgat ggaaaaatat ccggattaaa gaattaaaat
     2041 gaaaccggta gtattcccgg acacatagtt ttaccatact aaaatcagca ctaatgagta
     2101 ataatgaatc aaaccggaga aaattcatcc ggtcactggc cctgagtacg gcggccgttg
     2161 cggcaggtac ccgggcactc ggcaatgaaa acaaatcagc tcactttact attcagcaaa
     2221 aaagccgttt tactgcaaac gacaatatca atattgccct gatcggatcc ggcggtatgg
     2281 gagttcagga taccatcacg gccctgcagg ttcccggagt aaaactgata gccgtctgtg
     2341 atctgtatga tggccgttta caggaggcga agacccgctg gggcaaggat attattacca
     2401 cccgcagtta taaggaaatt ttaagtcgta aggatattga cgccgtgatt attgccacac
     2461 ccgatcactg gcaccagcag atttccattg acgccctgaa agccgggaaa catgtgtatt
     2521 gtgaaaaacc catggtgcat tccatacttg aaggaccggc cgttataaaa gcgcagaaag
     2581 aatccggaaa agtattccag gtgggcagcc agggtgtctc ctcacttggc aatgaaaagg
     2641 caaaagaact gctgaaaagc ggggcgatcg gggaattgaa ttatgcagaa ggattctggg
     2701 ccagacattc tcccacgggg gcctggcaat acccgattcc ggccgatgca tctccggcaa
     2761 atgtggattg ggaaacctat gtaagtaata ccgttaaacg accctttgat gccacccgct
     2821 ttttcaggtg gagaaattac aaggattacg ggacaggcat gtccggggat ctgtttgtac
     2881 atcttttttc cagtttacat tttataacca actctgttgg cccaagtaaa gtttacgcgg
     2941 ccgggggact ccggtactgg aaagatggcc gtgaagtacc ggatgtatta ctgggtactt
     3001 ttgattacca tcagcaccag gcgcatccgg ccttcaacct gtccctgcgt tgcaattttg
     3061 tggatggtac cagtggtacc acctacctga aactcgtggg cagcgaagga tccatggata
     3121 taacctggga tagcgtgacc ttaaaaagaa ataaaagctt tgcttcagat gatcccttct
     3181 tcctcgaaca gatgaagaaa gccggcactc cggtaaccgg aagaaaacgg attctgccac
     3241 cggaagaata tacatttgaa gccgagaaag gataccaggg tggtccgtat gatcattttg
     3301 taaacttctt taccgccata cggaatggag ggaacgttac ggaagacgca gtattcggct
     3361 accgggcagc cgcacccgca ctgctgtgca atgacagcta taaccaggac acagcgatat
     3421 tatgggatcc tgaaaaaatg caattagtaa aaaaataata aacagtatat gcaaataaaa
     3481 tcattgaaaa tcatggccgg gttaatcctg atgacaggaa tcgtttcctg taataatcag
     3541 gcagccggtg acaaatcaac taccgataca accgggaatg ctggtggaga taaaacagaa
     3601 gcagcaccat ctgcctgggt ggccattttt gacggagtaa gtcagaaggg ctggcatggc
     3661 tacaataaac agcctggtga actgaaaaac tggatgattg aagacagttc ccttgtttgc
     3721 ctgggagccg cagccgatgc acatggcggg gatattataa ccgataagac ctatacggat
     3781 tttgaactca cctgggactg gaaagtaacg aagggggcta atagcggggt catgtatcat
     3841 gtggtggaag acacggccta tcaggctccc tatgagaccg ggccggagta ccaggtaata
     3901 gatgatatcg gatttccgga taaactggaa gaatggcaga aagccggggc cgactacgcg
     3961 atgcataaca gcaatgaccg gaaaaaactc aagcccgccg gagaatggaa ttcaagtaaa
     4021 attattttca acaagggcca tgtggaacat tggttaaatg gcgaaaaaat cgttgacttc
     4081 caggcatggg atgcggaatg gactaagaaa aaggaggaag gcaaatggaa agattatccg
     4141 ggctacggaa cggcgaagac cggccatatt gcattgcagg atcatggcaa taaggtgtac
     4201 tacaggaata ttatgatcag ggaattataa gaagaacaat agatacggcc tgctcgcgga
     4261 aacatttagg gatctgcggg caggcttttt ttgtagctat ttacacaggc taaagaggga
     4321 gatccgtgtt gttttcatta tcacggtgaa atcacgaatg aagaccagct ggattatttt
     4381 ttttcgaagt tttgtatagg gaagtagggt gcgtaacata ttcatattca attgtttact
     4441 cttattaaag ttactgattt taaataactt agattaacta agcatttgaa aatcaatgtg
     4501 atttcgcacg aaagtgtcag tttttaggaa gtttttgtgc ccatttggtg cccaaataag
     4561 cgggcaacaa atggcgaaat catgcttcat acagctacat gtagctacaa tgagtagtaa
     4621 tatcagactt caagaaaact gtcaattttg tggcccgact tttatggcaa aaaccacggt
     4681 gaattaatgt tgttctgatg ttctttccaa agaatccgtt gccggaggat gaggatcgat
     4741 tgcagccacc aatctcagta caaaaattga cttacttaac ccatgattag gtaagaactt
     4801 ttaaaagacc agaaaaaaaa cgggtgcata aaattgctta tatttactat gcttaacgcg
     4861 gaagcatgcc actcgtctta gcaaagctgt atcagcgccg ccttcaaata aattatctat
     4921 cgaaaccaat ttctcgtttc gaaagatggt agacgggtga catttttccg gcgagagttt
     4981 tggataacga tggataccaa agtaaaaggt catacggccg catttcttaa aagacaagcg
     5041 aagaatctca aaaaaactaa aggcatttca tatctgagcg ccttagacca ggttgccttg
     5101 aatgccggtt ttaccaactg gaataatttt gtaaatcttc tgaatgctac taccgcagat
     5161 agcagaagta atcaatctgg taggaaagaa ccatccagat taagtcttac acaaaaccag
     5221 caggcatcag atataaatcc ttacaagaaa ttgctgatcg ctgcgctgaa tgaattatta
     5281 aaccgtaatc tgatttcatt aactgctcct aatgattcag tagatgaaaa gggacatgtt
     5341 tttttggaac tattcgggta tacttccgta gtgatgtggg atgatatcgg gtttgaagaa
     5401 ttgcggattt cggtttggtg gaagtatgat cattcaaagc acccccaggc gaatttgacg
     5461 gggaatgcca aagagagttt tagttgtgca tcgccattag ctaatagatt tcattacaaa
     5521 aactttgtcg gcatcactgc aagcggatgg ctggaaagaa aagtaggtaa ataccttcag
     5581 ggtaaaggca acagaggcat ttttgatatt tacacaagaa agggggaaaa aactgagctg
     5641 gagaaaatct caacgccaac acctaatggc tttggaatag aagggccagt gcacatgtaa
     5701 aatagataaa ttactttctg gctaatttat ggtaaggtta aagcaaacct ctgctttttg
     5761 aagggtagag gtttgctgat gcctcactac aacataccta cagataatga gattggagaa
     5821 aaaacaaagg cactgatcac cactgatctt tcagtggttt attcgctagt ttcatt
//
LOCUS       DAOPMW010000198         5871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15686_length_5871_cov_6.000516, whole genome shotgun sequence.
ACCESSION   DAOPMW010000198 DAOPMW010000000
VERSION     DAOPMW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15686_length_5871_cov_6.000516"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1882)
                     /locus_tag="PLU11_09820"
     CDS             complement(110..1882)
                     /locus_tag="PLU11_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN59462.1"
                     /translation="MKKTLFLALLLYTIATNSFGQPACPLKGDFSISRNPCAPFDINF
                     TTNISGHTNIRWNFGDAGTASGNTTISHTYASTGNYLVSVITDYPSCSDTVKKTITLD
                     ILPDQQLIQTADTIICSGSTKQLKSNPGFQFCWYPVTGLDNPASATPVCSVTQNTTYY
                     LNSYTTGVNLITNGNFNAGNSGFTSGYTYNPSSGFNPGMYTVTSNLQSWHPSMPACGD
                     HSTGSGNMMVVNGAEIPNIKVWAQTVTVQPNTNYAFSTWLQHVTSSNPAILQFSINDV
                     TIGPVFTANNTACIWDQFYTIWNSGNNTSAAIAIVNQNQVYTGNDFALDDISFAPVVF
                     SQDSIKITVDKPAIVSSPDQQICSGKSIQLNTTGGNSYSWTPASGLSSTSIPNPVASP
                     LTTTKYYVTGTNSNNCTGNDSVLITVNNLPTIEVSKSGDISCASTGSLLSATGAVQYS
                     WSPSTGLSNAAISNPLATPADTTSYIVTGTDANGCTNTGTITVNVTSTGKSLYLMPTA
                     FTPNGDGINDCYGINFWGTVNQLDFSIYNRWGQLVFHTSNPGECWNGYFKGELQDAAV
                     YVYVVKAKTLCGDTFKKGTFALIK"
     gene            complement(1907..3250)
                     /gene="purB"
                     /locus_tag="PLU11_09825"
     CDS             complement(1907..3250)
                     /gene="purB"
                     /locus_tag="PLU11_09825"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPN59463.1"
                     /translation="MELTPLTAISAIDGRYRKQVQHLDEYFSEYGLMKYRVLVEIEYL
                     LFLEKKKFFKLTPKAIKHLAKLAEDFGPEDAQEIKQIEFTTNHDVKAVEYFLKNELEK
                     CGAAEAKEWVHFGLTSQDINNTAIPLSWKHAIEYEYLPSLLNLNMELKLLVDEWRDIP
                     MLAHTHGQPASPTRLGKEIMVFAERIQNQIEQFIGVPFSGKFGGATGNFNAHHIAFPK
                     TDWIKFGNEFLQKLGLSRQQFTTQIEHYDSLAAHFDSMKRINNILIDFCRDIWTYVSM
                     EYFRQRTVKGEVGSSAMPHKVNPIDFENAEGNLGLANAILEHLSAKLPVSRLQRDLTD
                     STVLRNIGVPFAHTLIAFRSIEKGLSKLVVNDAKIYEDLDNNWAVVAEAIQTVLRREK
                     YPNPYEALKELTRGNNKIDKKAIHGFIDGLKVKETLKKELRKITPHNYTGISPFK"
     gene            3307..4050
                     /locus_tag="PLU11_09830"
     CDS             3307..4050
                     /locus_tag="PLU11_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPN59464.1"
                     /translation="MEKIRTGIVNYLNTRPLIYGLERQPVAGQIELIGDYPARIAAML
                     ISGEIDLGLIPVAVIPQLPEYHIVGDYCIGTEGEIASVALFSEVPMEEVEKVYLDYQS
                     RTSVALLRFLMKEYWGINPEIIQASGEDYRQEIKGHTAGLVIGDRALEQRKISTFIYD
                     LGSEWKKITGLPFVFAAWVSTRPLPEDFIRDFNQGNAMGLEKIDEIVAANPYPLYDLK
                     KYYKLHLSYQLDEAKIKGMQRFLEVIKDL"
     gene            complement(4054..4794)
                     /locus_tag="PLU11_09835"
     CDS             complement(4054..4794)
                     /locus_tag="PLU11_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPN59465.1"
                     /translation="MGFPFSGAIKSFLTRTFFRHVRRSYSQSGEDIIISDLFNRLQIK
                     SPRYLDIGANDPVSLNNTYRLYQRGASGVCVEPNPILCEKIRQKRSRDICINAGVAFD
                     EKREADFYVFPEKFNGLNTFSKEEADFWEKEGNDEIGKHKVEKVVRMQLLEINELMQQ
                     HFTPHPNLVSIDVEGFDFQIMQGIDFSRFRPEVFCIETLGFVEDNREIKKNDIIDFFL
                     SKGYFVYADTYINTIFCRKDSYTILKME"
     gene            4884..5789
                     /locus_tag="PLU11_09840"
     CDS             4884..5789
                     /locus_tag="PLU11_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPN59466.1"
                     /translation="MRTLIRNFLKRYGYDIVKTGVPYIPATNNDVLVTVGKFQVWMPG
                     YNVQAVNYRLYPDLNAQFGRLAKTIASKYPGMTLIDVGANVGDTIAVVKTEVDVPVIG
                     VEGDEVTFSYLEKNSKQFNNVSIVKTFLGEKSEELNVNFEKSGWNATIIPDDKGGKQV
                     KFKTLDAVISEGGFDNRTIKLLKVDVEGFDTIVLRGAGEIIKRHKPVVFLEYNRQNMI
                     SIKEEGLSTLLSFEQAGYNKVAFFDHKGTLILATSIKNKEVVTYLHDYASSVKNLIGY
                     YDICLFHEEDDHLAEQFLTLEKKFV"
BASE COUNT         1588 a         1205 c         1370 g         1708 t
ORIGIN      
        1 tgacaaaagg acagtaaata tgcttttttt catttgttgc ttttgctggt tttcaattct
       61 cattttatta gaatgaatca gcctttttat gtatttatga aaatctgttt tatttgatta
      121 atgcaaatgt tcctttctta aaggtgtcgc cacagagggt ttttgctttt acaacataca
      181 cgtagacagc tgcgtcctgc agttctcctt tgaaataccc gttccagcat tctccgggat
      241 tgctggtatg aaaaaccagt tgcccccacc tgttatatat gctgaaatcg agctgattaa
      301 cggtacccca gaaattaatg ccataacaat cattgatgcc atcgccgttt ggggtgaaag
      361 cggtaggcat caggtagaga ctttttccag tagatgtcac atttacggtt atagtaccgg
      421 tattcgtaca tccattggca tctgtgccgg ttactatata gcttgtggta tcggcgggtg
      481 ttgcaagcgg atttgagatc gctgcattgc tcaatccggt tgaagggctc caactgtatt
      541 gaacggcgcc ggtggcagat aacaggctgc cggtactcgc acaactgata tcacctgatt
      601 ttgaaacttc gatcgtgggc aggttgttca ccgtaatcag aacagaatca ttgcctgtgc
      661 aattattgct gttggttccc gttacataat atttagtggt ggtaagcgga gaagctaccg
      721 gattcggaat actggttgaa gagagcccac tagccggagt ccagctatag ctgtttccac
      781 ccgtcgtatt aagctgaatg gatttacctg aacagatctg ctgatcaggg gaactaacaa
      841 ttgccggctt gtcaacggtt atttttatgc tgtcctgact gaaaacaact ggggcaaatg
      901 aaatatcatc taaagcgaaa tcattccccg tataaacctg attttgattt acgatcgcaa
      961 tggccgcgct tgtattattg ccggaattcc agatggtgta aaattgatcc cagatacaag
     1021 cggtattgtt tgctgtaaaa accggaccaa tagttacatc gttaatggag aattgcagta
     1081 tggctgggtt agaagaggtg acgtgctgaa gccatgtgga gaaagcataa ttcgtattag
     1141 gttgtaccgt tacggtttgt gcccagactt ttatattggg aatttccgcc ccatttacga
     1201 ccatcatatt gccgcttccc gtgctatgat cgccgcaagc aggcatggat ggatgccagg
     1261 actgcaaatt ggaagttacg gtatacatac ccgggttaaa accggaagat ggattatacg
     1321 tgtatcctga tgtaaatccg gaattccccg cattgaaatt gccattcgtg ataagattca
     1381 caccagttgt ataagaatta agataatagg ttgtattctg tgttacagaa cagaccgggg
     1441 tggcagaggc cggattgtca agtcccgtta caggatacca acaaaattgg aaacccgggt
     1501 ttgattttaa ttgcttggta cttccggaac agataattgt atcagcagtc tgtatcaact
     1561 gctggtcggg aaggatatcg agcgtgatgg tttttttgac cgtatctgag caggaaggat
     1621 aatccgtaat tacgctaacc aggtaattcc cggttgaagc ataggtgtga gagatagtgg
     1681 tattcccact ggcagttccc gcatcgccaa aattccatcg gatattggta tggcctgaaa
     1741 tattggttgt aaaatttatg tcaaaggggg cacagggatt tctgctaata gaaaaatcgc
     1801 ccttcagggg acaggcaggc tgtccaaaag aatttgttgc aatggtatat agcaacaggg
     1861 caaggaataa ggtttttttc atgtaccaat aaatcagggc atgaatttac ttaaaagggc
     1921 ttatacctgt gtaattgtgg ggggtgattt ttctcagttc ttttttcagg gtttccttaa
     1981 ctttcaatcc atcaataaac ccgtgaattg cttttttatc gattttatta ttaccccttg
     2041 tcagttcctt caaagcttca tagggattcg ggtatttttc tcttcttaat acggtttgaa
     2101 tcgcctcggc tactaccgcc cagttattat ccaggtcttc gtagattttc gcatcattca
     2161 ctaccagttt gctcaatcct ttctcaatcg aacggaaggc aatcaacgta tgcgcaaagg
     2221 gtacgccgat attccgtaaa accgtagaat ctgtcaggtc tctctgcaaa cgggataccg
     2281 gtaatttggc cgacagatgt tccaggatgg cattggctag tccgagatta ccttccgcat
     2341 tttcaaaatc aatcgggttg actttatgcg gcatggctga cgaacccact tcgcctttta
     2401 ctgtacgctg gcggaaatat tccatggaaa cataggtcca gatatcccgg caaaaatcga
     2461 tcagaatatt gttgatcctt ttcatggaat caaaatgggc ggccaaactg tcataatgtt
     2521 cgatctgggt cgtgaattgc tggcgactca atccgagttt ctgtaaaaat tcattgccga
     2581 atttgatcca gtccgttttc ggaaatgcaa tatgatgggc gttgaaatta cccgtggccc
     2641 cgccaaattt tccggagaaa gggacgccga taaactgctc aatctggttc tgtatccgtt
     2701 ccgcaaatac catgatctct ttacctagtc tggttggact ggccggctgt ccatgggtat
     2761 gggccagcat cggaatatcc ctccattcat ccaccaacag tttcagctcc atattcaggt
     2821 tcagcaggga gggcaggtac tcatattcga tggcatgttt ccacgaaagg gggatcgccg
     2881 tattattgat gtcctgtgaa gtaagtccga aatgcaccca ttcttttgct tccgctgccc
     2941 cgcatttttc cagttcattt ttcaggaaat actccaccgc ttttacatca tggttggtgg
     3001 taaactcaat ctgcttgatc tcctgggcat cttcgggtcc gaaatcctcg gccagtttag
     3061 ccaggtgttt tatggctttg ggcgtgagtt tgaagaattt ctttttttcc aggaaaagga
     3121 ggtactcaat ctccaccaac acccggtact tcatcagtcc gtattcagaa aaatattcgt
     3181 ccaggtgctg aacctgtttg cgatagcgtc cgtcgatggc tgaaatagcg gtgagggggg
     3241 tcagttccat gtgagttgtt tatagataaa gatatccttt acctttgctg cgcaaaaata
     3301 acccaattgg agaagattcg cacaggcatt gttaattacc tcaataccag gcccctgatt
     3361 tatgggctgg aaagacaacc ggtggccgga cagattgagc tgatcggtga ttatccggcc
     3421 aggattgctg caatgctcat cagcggtgaa attgatctcg gcctgatccc ggtggccgtg
     3481 atccctcaat tacccgaata tcatattgtc ggggattatt gtatcggtac tgaaggggaa
     3541 atcgcttcag tagctttgtt cagcgaagtg cccatggaag aagtggagaa agtatacctt
     3601 gattaccaaa gccgcacctc ggtggccttg cttcgtttcc tgatgaaaga atattggggg
     3661 atcaatccgg aaatcataca ggcttccggt gaagattacc ggcaggagat caaggggcat
     3721 actgcaggat tggtcattgg tgaccgggcg ctggagcaac gaaaaatttc cacttttatc
     3781 tatgacctgg ggtcggaatg gaaaaaaatt accggacttc cctttgtatt tgcggcctgg
     3841 gtcagtaccc ggcctttacc cgaagacttt atccgtgatt ttaatcaggg gaatgccatg
     3901 ggactggaaa agatcgatga aattgtggcg gccaatcctt atccgcttta tgaccttaaa
     3961 aagtattata aactgcacct aagctaccaa ttggatgagg ccaagataaa gggaatgcag
     4021 cggttcctgg aagtgattaa ggatttataa tcgtcattcc attttcagga tggtataact
     4081 gtccttcctg caaaaaatgg tattgatgta ggtgtctgca tagacaaaat accctttgct
     4141 gagaaaaaaa tcaatgatat cgttcttttt gatctcccgg ttatcttcca caaatcccag
     4201 ggtttcaata caaaaaacct cgggcctgaa acggctgaaa tcaattcctt gcatgatctg
     4261 aaaatcgaaa ccttccacgt caatcgaaac caggttggga tgtggtgtaa aatgttgctg
     4321 catcagttcg ttgatctcca gcaactgcat tctcactact ttttccactt tatgctttcc
     4381 gatttcatcg tttccctctt tttcccagaa atcagcttct tcttttgaga atgtattcag
     4441 tccgttaaat ttttcaggaa aaacataaaa gtccgcttcc cttttttcat caaatgctac
     4501 tccggcgttg atgcagatat cacggctcct tttttggcgg attttttcac acaggatggg
     4561 gttgggttct acacaaaccc cgctggcacc ccgttggtac agccggtagg tattgttgag
     4621 cgaaacaggg tcattggccc cgatatccag gtaacggggg gactttatct ggaggcggtt
     4681 gaataaatca ctaataatta tatcttcgcc actttgggaa tagcttctcc ttacatgacg
     4741 gaagaatgtg cgggttaaaa aggatttgat agcaccagaa aaggggaagc ccatgggtta
     4801 aatgtattat agccgccaaa gatagtgaag caggcagaaa caggaagtct gccggctgat
     4861 tttagtaaaa tttgatacag attatgagaa cattaatcag gaatttctta aagcgttacg
     4921 ggtatgatat tgttaaaacc ggggttccct atattccggc caccaataat gatgtattag
     4981 taacagtagg aaaattccag gtatggatgc ctggctataa tgtacaggcg gtcaattacc
     5041 ggctttatcc ggatctgaat gcacagtttg gaaggctggc taaaacaatc gcatcaaaat
     5101 atccgggtat gaccctgatt gatgtgggag cgaatgtcgg cgatacgatt gccgtggtga
     5161 aaacagaagt ggatgttcca gtaatcggcg ttgaaggaga tgaagtgact tttagttacc
     5221 tggagaagaa cagtaagcag tttaataatg tctcaattgt taaaactttt cttggtgaaa
     5281 agtcagaaga actgaatgta aattttgaaa aaagtggctg gaatgccacc atcattccgg
     5341 atgacaaagg gggtaaacag gtaaagttta aaacgctgga tgccgtgatc agcgaaggtg
     5401 ggtttgacaa ccgtacgatc aaattgctga aagtagatgt ggaaggattt gatacaatcg
     5461 tattacgtgg agccggagaa atcattaaaa gacacaagcc ggttgtattc cttgaatata
     5521 accgtcagaa tatgatttct atcaaagaag aaggactttc cacattattg tcttttgaac
     5581 aggccgggta caataaagtt gccttttttg atcacaaagg caccctcatt ttggctacct
     5641 caattaagaa taaagaagtg gttacttacc tgcatgacta tgcttcttct gtaaaaaatc
     5701 tgatcggcta ttacgatatc tgtcttttcc acgaggagga tgaccatctg gcagaacaat
     5761 ttttaacgtt ggaaaaaaag tttgtgtaat cacttagcaa gctgatccaa ctgcttcacc
     5821 agcttctcaa actgcggctg gtacaggaaa tcataggcat tgggcccttc g
//
LOCUS       DAOPMW010000199         5866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15702_length_5866_cov_9.466873, whole genome shotgun sequence.
ACCESSION   DAOPMW010000199 DAOPMW010000000
VERSION     DAOPMW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15702_length_5866_cov_9.466873"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="radC"
                     /locus_tag="PLU11_09845"
     CDS             complement(<1..601)
                     /gene="radC"
                     /locus_tag="PLU11_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPN59467.1"
                     /translation="MQEHKYPINQWAKDDRPREKLRYTGAENLSHSELLAILIQNGTR
                     QKSAIDLAKEVLKLGKDNLVELGKLSVKDLMSIKGIGEAKAITIAAALELGRRQQAAA
                     PLPRELVRSSQDIASILQARFSHLRHEVFAVLYLNRANKIKQLEVVSEGGITGTVADP
                     RIILRKALEQDAVSLVLCHNHPSGSLRPSRADEQLTLKIK"
     gene            706..927
                     /locus_tag="PLU11_09850"
     CDS             706..927
                     /locus_tag="PLU11_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59468.1"
                     /translation="MKTKTLTPMGSLHPIIFFVVVYVVALLLSIFICSSLFYTFSGSS
                     RASGQIQPLEEAVTTSNTVVTASTVSLQR"
     gene            1011..1964
                     /locus_tag="PLU11_09855"
     CDS             1011..1964
                     /locus_tag="PLU11_09855"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPN59469.1"
                     /translation="MQYAKIFAGTGSQHLAEQICKRYGTKLGKVNIQRFSDGEISPIF
                     LESVRGDYVFLVQSTFSPAENLMELLLMIDAARRASAYKVIAVIPYYGYARQDRKDRP
                     RVAIGSKLVANMLVAAGADRVITMDLHAPQIQGYFDIPVDHLDSHAVFIPYIESLKLE
                     NLTFAAPDVGATNRIREIASYFNAEMVICDKHRKRANEIASMVVIGDVKDRDVVIIDD
                     ICDTGGTLAKSAGLLKEKGARTVRALITHPVLSGKAYQNIENSVLSELVVCDTIPVKQ
                     DTETDKIKVLSVAELFAVAIRNAYENKSITSLFIHSQRRDK"
     gene            1985..2623
                     /locus_tag="PLU11_09860"
     CDS             1985..2623
                     /locus_tag="PLU11_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPN59470.1"
                     /translation="MKTITIEGQLRTGFGKSASRQLRSQDMVPGVIYGGSAEINFYAP
                     TAAFKNFVYTPSFQIAEVVLDGKTYRTILKDLQFDKVDDKLIHIDLLELVEDKKVIAD
                     IPVKFVGTSKGVKDGGKLVTKMKSLKIKTYPKYLKEQIEVAIDNLELNGNIRVEDVKE
                     PNYEILNSPRIPVASVVLTRQLKQEEATAPAGAAKPAAAAAAPAAAKPAAKK"
     gene            2703..3278
                     /gene="pth"
                     /locus_tag="PLU11_09865"
     CDS             2703..3278
                     /gene="pth"
                     /locus_tag="PLU11_09865"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPN59471.1"
                     /translation="MKFLIAGLGNPGAEYAHTRHNIGFDVVNAFVQKHGGQFRSDRLA
                     FVADVKWKGKNFVCICPTTFMNLSGKAVKYWMDKEKVALEQVLVVVDDLALPLSKLRL
                     KPSGSDAGHNGLKSLQEILGTKDYPKLRFGIGSDYPKGLQADFVLGKWKKEEEPLVKL
                     KIEKSVEAIEMFAAQGLTAAMNQVNNKEFSL"
     gene            3452..4063
                     /locus_tag="PLU11_09870"
     CDS             3452..4063
                     /locus_tag="PLU11_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPN59472.1"
                     /translation="MKIFCVGRNYVAHAKELGNEVPDEPVIFMKPKSALLQPHTPFYY
                     PEFTNELHYECELVLRISKNGKYIQDKFASKYYDAITAGIDFTARDIQNELKEKGLPW
                     EKAKAWDNSAVIGKWVPFANIKNKKDINFCLYKNKELVQQGNSTLMIHSFDKIVSYIS
                     NFFSVNIGDIIFTGTPAGVGEVVVGDELEGFIEDDSMFALEVK"
     gene            4204..>5866
                     /locus_tag="PLU11_09875"
     CDS             4204..>5866
                     /locus_tag="PLU11_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPN59473.1"
                     /translation="MTELSILLRAYKLMSQTRAMADLYEANRSICKYVHSTSRGHEAI
                     QLATAFNLQSQDFVSPYYRDESMLQGLGFTPYEQMGQLLAKGDDIFTGGREYYSHPNY
                     RGDDKPLIIHQSSATGMQAIPTTGIAQGIRYWEKSDPARLKKGLNGELPVVLCSMGDA
                     SVTEGEVSEAMQFAVLKQLPVIYLVQDNNWGISVSAGEARAMNAYEYVAGFKGMNRIQ
                     VDGSDFEASYKVMKDVCEFVRRERKPVLVHAETPLLGHHTSGVRSDFYRSQVDLEKHH
                     QHDPIPRLRKRLEERGVSEAELSEIERAAEERVTADFKKAVAAPDPNPLTVGDHVFAP
                     SPVIEESGERSPANAEKVLMVDAALYAIRELMEDNPDMIIYGQDVGKRLGGVFREAAT
                     LAEQFGDHRVFNTAIQEAYIVGSTVGLNAVGIKPVVEVQFADYIYPGFNQLVTEISKS
                     CYLTMGKFPISMVLRVPIGAYGGGGPYHSGSVETTLLSIKGIKVVYPSNAADMKGLMK
                     AAYADGNPVVMLEHKGLYWSKVPGTEEAKMVEPSRDYIIPLGKASI"
BASE COUNT         1590 a         1282 c         1511 g         1483 t
ORIGIN      
        1 ctttgatctt cagggtgagt tgttcgtcgg cacggctggg gcggaggctg ccggaagggt
       61 ggttgtgaca gagaacaagg ctgacggcat cctgttccag ggctttgcgg agaatgatac
      121 ggggatcggc tacagtgccg gtgattcctc cttcactgac cacttccagt tgtttgattt
      181 tattggcccg gttgaggtag agtacggcaa acacttcgtg ccgcaggtga ctgaaacggg
      241 cctggagtat actggcaata tcctggctgc ttctgaccag ttcgcggggc aggggagcgg
      301 ctgcctgttg gcggcggccc agttcaagtg ccgcggcaat agtgatggcc ttagcttccc
      361 cgataccttt aatggacatg agatccttta cactgagctt gcccagttcc acgaggttat
      421 ccttccccag tttcagcact tctttggcca ggtctatagc agatttctga cgggttccat
      481 tctgaattag gatggccagc aattccgaat gactgaggtt ttctgcaccg gtgtatcgta
      541 atttttcgcg tggtctgtcg tcttttgccc attgattaat cgggtacttg tgctcttgca
      601 tgcaacaacg atagtcaata attttacatt cgtcaatacc actttcaggt attgttcagg
      661 cccgttccac atccactcta tgaagatcat taaaatctga tgaacatgaa aaccaagacc
      721 cttactccca tgggatcctt acatcccatc attttctttg tagtagttta tgtagtggct
      781 ttattgctgt ctatttttat ctgctcctcc cttttttaca cgtttagcgg ttcttcccgt
      841 gcttccggtc aaattcagcc tttggaagag gcggtaacta ccagtaacac ggtggtgaca
      901 gccagtacgg taagcttgca gcgctgatcc tgtaatcacg tcaatatttt tggcagtgtc
      961 atgcggtgat tgcggtgaga gtaatatctt cgccgcacat tcaatctgcc atgcaatacg
     1021 caaaaatatt tgccggtacc ggctcacaac acctggctga acaaatctgc aaacgttacg
     1081 ggactaaact gggtaaagtc aatatccagc ggttcagtga cggagaaatc agcccgattt
     1141 tcctcgaaag tgtccgtgga gactatgttt tcctggttca aagcactttt tcccccgccg
     1201 aaaacctgat ggagctgctg ctcatgatcg atgcagcccg ccgggcatct gcttacaaag
     1261 taattgccgt tattccttat tatgggtatg cccgccagga ccggaaggac cgtccccggg
     1321 tagccatagg tagtaagctg gttgccaata tgttagtcgc tgccggagcg gaccgggtga
     1381 ttaccatgga cctccatgct ccccagattc agggttattt tgacataccg gtggatcacc
     1441 tggacagcca cgcagttttt atcccttata tagagagttt gaagctggaa aatcttacct
     1501 ttgccgcccc cgacgtagga gcgaccaacc gtatccggga gattgccagt tatttcaatg
     1561 cagaaatggt gatctgcgac aagcaccgga aacgggctaa tgagattgcg agtatggtgg
     1621 tgatcgggga tgtgaaagac cgggatgtgg tgattattga tgatatctgt gataccgggg
     1681 gtaccctggc gaaaagtgca ggtcttttaa aagaaaaagg agcccggacc gtaagagccc
     1741 tgattacgca cccggttttg agcggcaaag catatcagaa tatcgagaac agtgtgcttt
     1801 ccgaactggt ggtatgcgat acgatacccg taaaacagga tacggagaca gataaaatca
     1861 aagtgttgag tgtggctgaa ctttttgcgg tagcgatcag gaatgcctat gagaataaaa
     1921 gtatcaccag cctgtttatc cattcgcaac gaagagacaa gtaaactata aaacaacaat
     1981 aaaaatgaag acaattacaa tcgaaggaca actgaggacc ggattcggca aaagcgccag
     2041 ccgacaactc cgctctcagg atatggtgcc aggtgtaatt tatggcggct cagcggagat
     2101 taatttctac gctcctactg cagctttcaa aaatttcgtg tacacaccaa gtttccagat
     2161 cgctgaagta gtgctggatg gcaagaccta ccgcactatc ctgaaagacc tgcagtttga
     2221 caaagtggat gacaaactga tccatattga tctgctggaa ctggtagaag ataaaaaagt
     2281 aatcgctgac atccctgtga aatttgtggg tacttctaaa ggagtaaaag atggtggtaa
     2341 actggtgacc aagatgaagt cactgaagat taaaacctat cctaaatacc tgaaagaaca
     2401 gattgaagta gctattgata acctggaact gaacggtaat atccgtgtgg aagatgtaaa
     2461 ggagccgaat tatgagatcc ttaactcccc ccgtattccg gttgcgtctg tggtattgac
     2521 ccgtcagctg aaacaggaag aagcaactgc acctgccggt gctgcaaaac ctgccgctgc
     2581 tgccgctgcc cccgctgctg cgaagccagc cgccaaaaaa taaggtttta cacttatatc
     2641 ataaacccgt atcaactgat gcgggttttt tattggctat caccgatatt tgagatacga
     2701 ccatgaaatt tttaatcgcc ggactgggta atccgggcgc agaatacgcc cacacccgtc
     2761 ataatattgg atttgatgta gtgaatgcct ttgtacaaaa gcatggggga cagttccgga
     2821 gtgaccggct ggcctttgtg gctgatgtga aatggaaggg gaagaatttt gtctgtatct
     2881 gccccactac tttcatgaac ctgagcggga aagcggtgaa atactggatg gataaagaaa
     2941 aagtagccct ggaacaggta ttagtggtgg tggacgacct ggccctgccc ctgagtaaac
     3001 tgcgtctaaa gccttctggc agcgatgcag ggcataatgg cctgaaaagc ctccaggaaa
     3061 tcctgggaac gaaggattat cccaaactcc ggttcggaat cgggagcgat taccccaaag
     3121 gattacaggc ggattttgtg ctgggaaaat ggaaaaaaga ggaggagccg ctggttaaac
     3181 tgaagatcga aaagtcggtg gaagcgattg aaatgtttgc ggcacaaggg cttactgccg
     3241 ccatgaacca ggtaaataat aaagagtttt cattatgaga aatcagatgg aatccttact
     3301 ttagcactcc tgacctctcc ccggaaggcg cctgattagc aattttttac cggggaaatc
     3361 caatatacat attgattacc actgaatata tgggatcttg tcaccccagg tagggtatcc
     3421 ggtatattta attaaaatgt tacttcctgt tatgaaaatt ttctgtgttg gacgaaacta
     3481 tgttgcacac gcgaaagaac tcggaaacga agtgcccgat gagcctgtga tattcatgaa
     3541 gcccaaaagt gctttattgc aaccgcatac gccattttat tatcccgaat ttaccaatga
     3601 actgcattat gaatgcgaac tggtgctgcg tatcagcaag aatggtaaat atatccagga
     3661 caaatttgct tccaaatatt atgatgccat tacggccggt atcgacttta ctgcccgtga
     3721 tatacagaat gaattgaaag aaaaaggact gccctgggaa aaagcaaaag cctgggataa
     3781 ctccgcggtg attggcaaat gggtaccgtt cgccaatatc aagaataaaa aggacatcaa
     3841 tttctgcctg tacaagaata aagaactggt acagcaggga aactccactc tcatgatcca
     3901 tagttttgac aagatcgtgt cttatatctc caacttcttt tctgtaaata tcggggatat
     3961 catttttacc ggcacccccg caggtgtagg ggaagtggtg gtaggagatg agctggaagg
     4021 atttatcgaa gacgacagta tgttcgcgct tgaagtaaaa taagcgggac tcaggatttt
     4081 tatattccga caaaaggagc accaatacca actgctatca gggggttgta tcaaaaggag
     4141 atacaacccc ttattttttc tgaaccgctt tatcttactt ttgttttgta atgtttgctg
     4201 cagatgactg aactatccat tttactccgc gcatataagc tcatgagcca gaccagggcc
     4261 atggccgatt tgtatgaggc taaccgctcc atttgtaaat acgtgcattc cacttcgcgg
     4321 ggacatgaag ccattcaact ggccaccgct ttcaatttac aatcacagga ttttgtcagc
     4381 ccttattacc gggatgaaag tatgctgcag ggactgggtt ttacgcccta tgaacaaatg
     4441 gggcaactcc tggcaaaagg ggatgatatt tttactggcg ggagggaata ctattcccat
     4501 ccgaattacc ggggcgatga caagccgctg atcattcacc agagcagtgc cacgggtatg
     4561 caggccatcc ccaccaccgg tattgcccaa gggatacggt actgggaaaa atcagatcct
     4621 gcccgcctga agaaaggcct gaatggagaa ttgcccgtag tactctgttc catgggtgat
     4681 gccagtgtga ccgaaggaga ggtgagtgag gctatgcagt ttgccgtatt aaaacaatta
     4741 ccggtgatct atctcgtgca ggataataac tgggggatta gtgtatcagc cggagaagca
     4801 cgcgccatga acgcgtatga gtatgtagcg ggatttaaag gcatgaaccg gatacaggtg
     4861 gatggaagtg attttgaagc ttcctataaa gtgatgaaag atgtttgtga gtttgtacgc
     4921 cgggaaagaa agcccgtgct tgtgcatgca gaaaccccgt tgttgggaca ccatacgagt
     4981 ggtgtgcggt cggactttta ccgcagccag gtagatctgg aaaaacatca tcagcatgat
     5041 cctattcccc gtttacggaa gcgactggag gaaagagggg tatctgaagc tgaattaagt
     5101 gaaattgaaa gggccgcgga agaacgcgta acggctgatt ttaaaaaagc ggtggcggcc
     5161 ccggatccga atccgctgac agtgggcgac catgtttttg ctccatcacc cgtaattgaa
     5221 gaaagtggcg agcgctcacc ggctaacgcg gaaaaagtac tgatggtgga tgccgccctc
     5281 tatgccatcc gcgaattaat ggaagacaat cccgacatga tcatctacgg acaggatgtt
     5341 ggcaaacgtt tgggtggtgt gtttcgcgaa gcggccaccc tggccgaaca gttcggagat
     5401 caccgggtat ttaatacagc gatacaggaa gcctatatcg tgggatcgac tgtaggactg
     5461 aatgcagtgg gcatcaaacc tgtagtggaa gtgcaatttg ccgattatat ttatccgggt
     5521 ttcaaccagc tggtgacaga gatatccaag agctgttacc taacgatggg caaattcccg
     5581 atctccatgg tattacgggt acctataggc gcatatggtg gcggcggtcc gtatcatagt
     5641 gggagtgtgg aaaccacttt gctttccatt aaaggaatta aagtggtgta tccttctaat
     5701 gcggcggata tgaaagggct gatgaaagcc gcctatgcgg atggcaaccc ggtagtgatg
     5761 ctcgagcata aaggacttta ctggagtaaa gtgcctggta cggaagaagc taaaatggtg
     5821 gaaccgtccc gcgattatat tattccctta gggaaggcca gtattg
//
LOCUS       DAOPMW010000200         5862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15719_length_5862_cov_7.358188, whole genome shotgun sequence.
ACCESSION   DAOPMW010000200 DAOPMW010000000
VERSION     DAOPMW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15719_length_5862_cov_7.358188"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLU11_09880"
     CDS             complement(<1..697)
                     /locus_tag="PLU11_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HPN59474.1"
                     /translation="MTIGLLKEPAHETRVSLLAEAVATLTKKGITVLTETGAGERAFC
                     SDADYVKAGATVKSRAEVLQSAELLLTIHQPDPGEISGLSGKILIGVYQPLFNTDQMK
                     TWAVGGLTSFSLDMLPRTTRAQAMDVLSSQANIAGYKAVLTAANAYGRYFPMFMTAAG
                     SIAPAKMLILGAGVAGLQAIATSRRLGAVVEVFDTRPAVKEEVMSLGAKFIEVEGAAD
                     ASKAGGYAVEQSED"
     gene            complement(819..1616)
                     /locus_tag="PLU11_09885"
     CDS             complement(819..1616)
                     /locus_tag="PLU11_09885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN59475.1"
                     /translation="MYSLPQLALRYLQYRLRASNGRGHGMHSPFVFDFITRVLNDRSL
                     YPAYKDIESLRHQLKQDRQVIGVEDFGAGSVYRSQQQRSIGSIVRNAAKPAKYGQLLH
                     RMVKYFQPANILELGTSLGITSAYLATAAPGSRVVTMEGASEIASRARKNFSMLGLKN
                     VQVVEGNFEVTLPAFLETTGVIDFAFIDGNHRKEPTLRYFEQLLAHQGNESIFIFDDI
                     HWSAEMEEAWEIIRKHPSVQCSIDLFFIGIVVFRKEFREKQQFEIRF"
     assembly_gap    1727..1826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1827..2320)
                     /gene="thyA"
                     /locus_tag="PLU11_09890"
     CDS             complement(<1827..2320)
                     /gene="thyA"
                     /locus_tag="PLU11_09890"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPN59476.1"
                     /translation="MQNYLALLQHILDQGTDKTDRTGTGTRSVFGYQLRYDLQKGFPL
                     VTTKKVHLKSIIYELLWFLRGETNIAYLKENGVSIWNEWADDNGELGPVYGKQWRSWE
                     GKDGKVIDQVTELIAQIKKNPDSRRLIINAWNVADLPEMALMPCHTMFQFYVADGKLS
                     CQLY"
     gene            complement(2399..3298)
                     /locus_tag="PLU11_09895"
     CDS             complement(2399..3298)
                     /locus_tag="PLU11_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59477.1"
                     /translation="MRNLLLITALIWVVELNSQGNVLPSGELGTSFLSSLQLGKFGPL
                     EKYIPTVAFYKSLGKEMAGRSDKEIGQLRDQSTTRLKENWKQILERVRKDGIDLAGVL
                     IRETLVYDIMPGKPMQGMVVVYGYKGHEYDDLSLIVNQQPGKTWLLEIPNSTRVFKMD
                     DSTLRNSKQARQVIALSDPQLKKQLESQVKAMIRQAKGDSLQAFGSQVIFRGEPASKN
                     WKSALNMNLPEEVEQAERLMKQVSKAMAGCSDYKMESMRVEKESEGNWLVQPVKCGSK
                     KVYFAFLMTGSGLLLGDLDTESE"
     gene            3477..3830
                     /locus_tag="PLU11_09900"
     CDS             3477..3830
                     /locus_tag="PLU11_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59478.1"
                     /translation="MFYAIAAADKKIRPEEKKTLHDEILFAWKHHENSTDRFGSDRAF
                     LIEFEFETLEDDAVTAENAYQLFETYYLGNEKNFDAASRIKIFNSARHIAECVRKINH
                     EELNYLVRLKALLKL"
     gene            complement(3831..5102)
                     /locus_tag="PLU11_09905"
     CDS             complement(3831..5102)
                     /locus_tag="PLU11_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPN59479.1"
                     /translation="MGLWINFGATYLRFMDNKYPDISFDNTEFAFEYKSDKDLKKAHF
                     LFTMMGKPWLVKIGTRLAPWAINMGLPVKGMIRNTIFSQFCGGESLEKTVGVAQMLAK
                     YNVKIILDYGVEGKEGEANFDQASDVFIKVIDYAATQPNIPFMSVKVTGFARHALLEK
                     IDSMMKAGEGSLNNRFEKALYDLVPEERSEWDRIHHRVNRVCAAAAAKSIGVSIDAEE
                     TWIQDPVDMLTILMMEKYNKEKPIVYNTLQHYRHDRLKFLEDSYNAAVAHNFILGSKL
                     VRGAYMERERRRAAEKGYPSPIQPDKESTDRDFNAGVKFCIDHIDRVALVVASHNEYS
                     NFYATQLLKEKGLPLSHPYAHWSQLYGMSDNITFNLAHAGCSVSKYLPFGPIKDVIPY
                     LMRRAQENTSVKGQTGRELGLIDRELKRRKL"
     gene            complement(5123..5839)
                     /locus_tag="PLU11_09910"
     CDS             complement(5123..5839)
                     /locus_tag="PLU11_09910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN59480.1"
                     /translation="MESLKGKSVLITGATGGIGAATARLLAASGAQVFITGRNEERLK
                     ALQQELQLPADRFFAADLSSASEVDKLKERFHAVFPTIDILVNAAGVGIIKPIDTLTG
                     EEFMTTLQVNLVAPFLLMKAFLPAMKDVKKGLIINIPGVLGKVPMAGAAAYSASKYGL
                     TGMMQSIREELKRTDIRITNLFLGGVDSAFWDHIDLRVQREKMIRAEEAAKSVWFLCQ
                     QPLSGVVSEMVLQPFNHQAI"
BASE COUNT         1464 a         1507 c         1212 g         1579 t
ORIGIN      
        1 aatcttcgga ttgctccacg gcatacccac ccgccttgct cgcatccgcg gcgccttcca
       61 cttctataaa tttggctccg agactcatga cttcctcttt aactgcagga cgggtatcaa
      121 atacttccac tactgctcct aaacgtcttg atgtggcaat ggcttgtaaa ccggccacgc
      181 ctgctccgag aatcaacatc ttcgccgggg cgatactacc cgccgctgtc ataaacatcg
      241 ggaagtaacg gccataggca ttggccgcag taagcaccgc tttataaccg gcaatattgg
      301 cctgcgaact cagcacatcc atggcctgtg cccgggtggt acgcggcagc atatccagag
      361 aaaaagaagt cagccccccg accgcccagg tcttcatctg gtctgtattg aacaagggct
      421 gatatacacc gattagtatt ttacccgata atccgcttat ttcaccggga tctggctgat
      481 ggatcgtcaa cagcagttcg gccgattgta atacttccgc ccggcttttt actgtagcac
      541 ccgctttgac ataatccgca tcgctgcaga aagcccgctc tccggcaccg gtttcggtaa
      601 gtacagtaat tccttttttt gtcagtgtgg ccacggcttc tgccaggagg gagaccctgg
      661 tttcgtgggc tggttctttc aataaaccga tcgtcataag ttgatcttgt taggagagaa
      721 aatagaattt tctatagatg tgaagatatt gaatttcctc cggagggacg ccttcaattg
      781 cattgatttt cttcaccttc gcagctgttg ttcgtcagtc agaaccggat ttcaaactgt
      841 tgtttttccc tgaattcctt ccggaaaacc acgattccga taaaaaacag atcaatgctg
      901 cactgtacag atggatgctt ccggatgatt tcccatgctt cctccatctc cgcactccag
      961 tgaatatcat caaagatgaa aatactctcg tttccctggt gggccagcaa ttgttcaaaa
     1021 taacgaagag ttggttcttt ccggtgattg ccatcgatga aagcaaaatc aatcacccct
     1081 gttgtctcca gaaaagctgg cagggtgacc tcaaaattac cttccactac ctgcacattt
     1141 ttaaggccca gcatggaaaa attcttgcgg gccctgctgg caatttcact ggcgccttcc
     1201 attgtgacca cccgggagcc gggagccgca gtggccaggt aagcactggt aatgcccaac
     1261 gatgtaccaa gctcaagtat gtttgccggt tgaaaatatt ttaccatacg gtgcagtaat
     1321 tgcccgtatt tcgcaggctt ggctgcatta cggacaattg acccgatgct ccgctgttgt
     1381 tgggagcggt acacagaacc agccccgaaa tcctcaaccc cgatgacctg ccggtcctgt
     1441 tttaactgat gacgtaatga ttctatgtct ttataagccg ggtaaaggct gcggtcattc
     1501 aacacccggg taataaaatc aaatacaaaa ggagaatgca tgccatgtcc ccggccattc
     1561 gaggcacgta accggtattg caggtagcga agcgctaatt ggggaagact atacatgtac
     1621 tgttgattga aaactatccc agtctttccc attgctggaa agaataattg aagggatttt
     1681 tctcatccgc ttcatgattg cgctgactga tcagctgcca ctgtttnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnntggt acaactggca actgagttta ccatcggcca
     1861 catagaattg aaacatcgta tgacagggca tcagggccat ttccggaaga tcggctacat
     1921 tccaggcatt gatgatcagc cggcggctgt ccgggttctt tttaatctgg gcgatcagtt
     1981 ccgttacctg gtcaatcact ttaccatcct tgccttccca gcttctccat tgtttaccgt
     2041 acacgggtcc cagttcgcca ttatcatcgg cccattcatt ccagatagag accccatttt
     2101 ccttaagata agcaatattg gtttcacccc gtaaaaacca gagcagttca taaataatac
     2161 ttttcaggtg aacttttttg gtggttacca gcggaaatcc tttttgtaaa tcataccgta
     2221 gctggtatcc gaaaacacta cgggtgccgg taccggtacg atcggtttta tcggtaccct
     2281 gatccagaat atgctgcaat aacgctaagt aattttgcat ggctgcaagt tagggagaat
     2341 gctgcttgca gccgtgccgg gtagcaccct gtggaaaaat ccgggctact gccccgtatt
     2401 attctgattc cgtatccaga tcacccagca ataatcctga accggtcatg agaaaggcaa
     2461 agtatacttt cttactgcca catttcaccg gctgaaccaa ccagtttcct tccgattcct
     2521 tttccacccg catcgattcc attttatagt cactgcagcc ggccattgct ttactgacct
     2581 gcttcatcag tctttctgcc tgctccactt cttccggcaa attcatattg agtgcagatt
     2641 tccagttttt tgatgccggt tcaccccgaa aaataacctg tgacccgaat gcctgtaaac
     2701 tatccccttt tgcctggcgg atcatcgctt taacctggct ttccagctgc tttttcaact
     2761 gaggatctga aagcgcaatc acctgccggg cttgtttgct gttacgcagg gtggaatcgt
     2821 ccattttaaa tacacgggtg gaattgggga tctcgagcag ccaggttttt ccaggctgct
     2881 gattgacaat taaactcagg tcatcatact catggccctt atacccatac acgaccacca
     2941 ttccctgcat gggtttgcct ggcataatat catatacaag tgtttcacgg atcaaaactc
     3001 ccgctaagtc aatcccatcc ttgcggacac gctcaagaat ctgcttccag ttctctttta
     3061 atcgggtggt actttgatcc cttaactgtc cgatctcttt atctgaacgg ccggccattt
     3121 ccttgcccag tgatttataa aaggcaacag ttggaatata tttttccagc ggaccgaact
     3181 tacccagctg aagggaggat aaaaaagagg tacccagttc cccggagggc agtacattac
     3241 cctgactgtt taattcaaca acccaaatta aggcggttat aagcaggaga ttccgcatgt
     3301 ttgttttttt tgtaaaatac gaagcaagag tcgtttatta ataccctgaa gatggtatat
     3361 actgcgagca atccgcttca ggcagtagca tgataaaggt cattccttta tttccgaaaa
     3421 gattctagct ttaagatatg caaaacccaa gagaattata tagagaattg ggaaacttgt
     3481 tttacgctat tgcagccgcc gataaaaaaa tcaggcccga agaaaagaag acccttcatg
     3541 acgaaatact atttgcctgg aaacatcatg aaaacagcac agatcgtttc ggttcagacc
     3601 gggccttcct gatcgaattt gaatttgaaa cactggaaga tgatgctgtt actgctgaaa
     3661 acgcttatca gctatttgaa acctattacc tgggaaatga aaagaatttt gatgcggcca
     3721 gccgtattaa aatattcaat agcgcccggc atatcgcgga gtgcgttcgt aaaatcaacc
     3781 atgaagaatt aaattacctg gtccggttga aagccctgct gaaactctga tcagagtttt
     3841 cgccttttca attcccggtc gatcagtccc agttcacgcc cggtttgtcc cttcactgaa
     3901 gtattttcct gtgctctcct catcaggtac ggaatcacat ccttaatggg tccaaaaggc
     3961 aggtatttac tgacactgca accggcgtgt gccaggttaa atgtgatatt atcactcatg
     4021 ccatataact ggctccaatg cgcataagga tgactcagag gcaggccttt ttcctttaaa
     4081 agctgcgtgg cataaaaatt actatactcg ttatgagaag cgactacaag cgcaacccgg
     4141 tcaatatggt cgatacaaaa tttcaccccg gcattaaaat cacggtccgt actttcttta
     4201 tccggctgga tgggtgaagg ataccctttc tctgcagccc ttctccgctc ccgctccata
     4261 taagccccac ggaccagctt tgaacctaaa ataaaattat gggcaaccgc tgcattataa
     4321 ctgtcttcca gaaacttaag tcgatcgtgt cgatagtgtt gcagggtatt atatacaatt
     4381 ggtttctcct tattgtattt ctccatcatc aggatcgtca gcatatccac cggatcctga
     4441 atccaggttt cttccgcatc aatggacact ccgatcgatt tggcggcagc ggcggcacag
     4501 accctgttta cccgatgatg tattcgatcc cactccgacc gctcttcggg tacgaggtca
     4561 taaagcgctt tttcaaaccg gttattcaga ctgccttccc cggctttcat catggaatca
     4621 atcttttcca acagcgcatg acgggcaaat cccgttactt tcacactcat gaaaggtata
     4681 ttgggctggg tagccgcata atcgattacc ttaataaata catcactggc ctggtcaaaa
     4741 ttggcttccc cttccttgcc ctccaccccg taatccagta taatcttcac attgtatttg
     4801 gccagcattt gtgctacccc aacagtcttt tccagcgatt cgcccccgca aaactgggag
     4861 aaaatcgtat tgcggatcat accttttacc ggcaaaccca tgttaatcgc ccaaggtgcc
     4921 agtcttgtgc caatcttcac cagccagggc ttccccatca tggtgaaaag aaaatgtgcc
     4981 ttttttaagt ctttgtcgga tttgtactca aaggcaaact ccgtattgtc aaatgatatg
     5041 tcggggtatt tgttatccat aaaacgaaga taagtggctc caaaattaat ccaaagcccc
     5101 atacaaacat ttatgagttt tgtcagatcg cctggtgatt aaaaggctgc aataccatct
     5161 cactgaccac cccgctgagc ggttgctggc agagaaacca gacagatttt gccgcttctt
     5221 ctgcccggat cattttttcc cgctgtaccc gcaggtcaat atgatcccaa aaagcggagt
     5281 ccacccctcc caggaagaga ttggtgattc gtatatccgt cctcttcagc tcttcgcgga
     5341 tactctgcat cataccggtc aatccatact tactggcact gtaggctgca gccccggcca
     5401 ttggcacttt gcccaaaacc cctggaatat ttatgatcag cccttttttt acatccttca
     5461 tggctggaag gaatgccttc atcagcagaa aaggtgcaac caggttgacc tgcagggtag
     5521 tcataaactc ttcccctgtc agggtgtcaa tcggcttgat aatccccacc ccggctgcat
     5581 tgaccagtat atctatcgtg ggaaatactg catgaaaccg ctctttcagc ttgtctacct
     5641 ctgatgctga actgagatca gccgcaaaaa aacgatcagc cggcaattgt aattcctgct
     5701 gcaacgcttt taaccgctct tcattccggc cggtgataaa tacttgcgcc ccactggcag
     5761 ccagcaaacg ggctgtagcc gctccaatac caccggtggc cccggtgatc aataccgatt
     5821 ttcctttcag ggattccata taaactgttt acacgtcaac aa
//
LOCUS       DAOPMW010000201         5842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15800_length_5842_cov_9.630551, whole genome shotgun sequence.
ACCESSION   DAOPMW010000201 DAOPMW010000000
VERSION     DAOPMW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15800_length_5842_cov_9.630551"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLU11_09915"
     CDS             complement(<1..678)
                     /locus_tag="PLU11_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPN59481.1"
                     /translation="MQVEKFYYDNKIVKAFGIATIVFGIVGMLAGLWAAIQIYYPAIS
                     LNIAPTTFGRLRPLHTNAIIFAFVGNSIFTGVYYSLQRLCKARMFSDKLSWIHFWGWQ
                     AIIAAAAITLLMGITTSKEYAELEWPIDIAITLVWVIFGINMFGTILKRRERHLYVAI
                     WFYIATWVTVAMLHIVNSMEIPVSLTKSYSMYAGVQDALVQWWYGHNAVAFFLTTPVL
                     GLMYYFLP"
     gene            complement(703..864)
                     /gene="ccoS"
                     /locus_tag="PLU11_09920"
     CDS             complement(703..864)
                     /gene="ccoS"
                     /locus_tag="PLU11_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HPN59482.1"
                     /translation="MSVIIILMIASISVAAIFLVAFLWGVKSGQFEDDFSPSSRILFE
                     DKPASTKTV"
     gene            complement(982..3378)
                     /locus_tag="PLU11_09925"
     CDS             complement(982..3378)
                     /locus_tag="PLU11_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HPN59483.1"
                     /translation="MSSSVITEQEIHCYHCGETCLNTKIRMAEKVFCCDGCKMVYQIL
                     NQSELCEYYNLNDNPGINQRVRVRHDKFAFLDDEKIQLQLISFEDEKQVHITFYLPQM
                     HCSSCLYLLENLHRLNPGVVSSKVNFTRKEADIVFLKDKTSLRSVAETLTNIGYEPYI
                     SLNDLKEKRPAIDRSMVYQIGVAGFAFGNIMLMSFPEYLGIDASEKGLLTIFRWANFG
                     LAIPVLLYSALPFYISSWKSLKHKFLNIDAPIALAVIITFIRSAYEVISGTGGGYFDS
                     MTGIVFFMLAGRILQDKTYRQLSFERDYTSYFPIAISVLKEGKETPTALPDIKPGDTL
                     LIHNEELIPADGLLTRGKAFIDYSFVTGESLPVQKEIGEIVYAGGKQTEGNIEILVVK
                     EVTQSYLTKLWNRDEFKEQEEINTKSFVHLLSRYFTYIVLAIAAATVIYWQFNDPSRV
                     WPAMTSIFIIACPCALFLSNTFTNGNILRILGRNHLYLRNAQTIEDLGKATHIVFDKT
                     GTLTSSQEQDIVYEGKELSELQLQQVAALADHSNHPLSKALARHIGRKQGFDIDHFKE
                     IPGQGIEGQFGEDSLKLGSYVFTTGNTGHTSGTSVFVRFGDEVFGQFRFSNHYREAIP
                     ELIRELKKEYKLSVLSGDNMAEQENLQKLMGSNATLLFHQKPEDKLNYIRFLQEKGEK
                     VMMIGDGLNDAGALKQSDIGIAVAEQTNNFTPSSDAIIEAKKLSGLTRFIRLCRANRN
                     IVVASFVLSIVYNVIGLFFAVQGTLSPLIAAILMPSSSLSILLLTFGSSSLIAKRLKL
                     "
     gene            3526..4587
                     /locus_tag="PLU11_09930"
     CDS             3526..4587
                     /locus_tag="PLU11_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN59484.1"
                     /translation="MPQRTILIIDDNEDIRENTAEILSLGGYKTLTAENGKRGVEAAL
                     LEKPDLIVCDIMMPELDGYGVLHLLRKNPETENIPLIFLTAKAERSDLRKGMEMGADD
                     YITKPFEEIELMNAIESRLKKYEVLQKNYNASGKGLSELANDLRESGMLQFNPDNYNS
                     ELYTKKQVIYAEGKRPRFLYFLVKGKVKGFKTHEDGKEYITDLYSDGDFIGYPALIEE
                     KNYDDSAVALEDTEIVQVPREDFQQMIDGNISVASKFIRIITQNVKEKEERLLSLAYS
                     SLRKRVAKALVDIHGKFNSAGENKPIEISREDIAHYVGTATESLIRTLSDFKSEKLIE
                     IKDGKISISNIEKLKNLLY"
     gene            complement(4662..5114)
                     /locus_tag="PLU11_09935"
     CDS             complement(4662..5114)
                     /locus_tag="PLU11_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPN59485.1"
                     /translation="MESKALDKPRSSWPTLFDSGWMDKFFNAPIDEFFNMGKVMNVPA
                     VNVSETDKEFRLCVAAPGMEKKDFKVEAFDEMLTISAEKEKEEKEEKNGRFNRREYNY
                     SSWSRSFTLPENCDTAKIAAEYNNGELKILIPKLEINQPKKVKSISVN"
     gene            complement(5268..>5842)
                     /locus_tag="PLU11_09940"
     CDS             complement(5268..>5842)
                     /locus_tag="PLU11_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPN59486.1"
                     /translation="YYNSYEFIELMLSQPLSRTRILLSEYAGVCVSLLSSFWIGVGVP
                     VILYAPDATGFALLFTGTSLTVVFTSIAFLAAVRSRDKARGIGFALLLWFYFALIYDG
                     LVLLTLFAFSDYPMEKFTLLLSALNPIDLGRIYIMLKMDISALMGYTGALYKDFFGSG
                     SGVLFTAGIMALWGIVPLWLALRSFKRKDM"
BASE COUNT         1688 a         1315 c         1327 g         1512 t
ORIGIN      
        1 gggcaggaag taatacatca gacccagcac cggggtagtc aggaaaaagg ccaccgcatt
       61 gtgaccatac caccattgta ccagggcatc ctgcacaccg gcatacatac tgtaactctt
      121 ggtcagggaa accgggattt ccatggagtt gacaatatgc agcatggcta cggtaaccca
      181 ggtagctata taaaaccaga tggccacata aagatgacgt tcccttcttt tcaggatcgt
      241 accaaacatg ttgataccga aaatcaccca tacaagtgta atcgcaatgt cgatcggcca
      301 ttcgagctct gcatattcct tactggtagt aatacccatc aataaagtaa tagctgcagc
      361 tgcaataatg gcctgccagc cccagaaatg gatccaggat aatttatcac tgaacatccg
      421 ggctttacaa agccgctgaa gggaatagta gacccctgta aaaatggagt ttccgacaaa
      481 tgcaaaaatg atggcattgg tgtgcaaagg acggagacgg ccaaatgtgg tgggagcaat
      541 attcagactg atcgccggat agtaaatctg gatagcagcc cataatcccg ccagcatgcc
      601 tacgatacca aacacgatcg tggcaattcc gaatgccttc acaatcttgt tgtcataata
      661 gaatttttct acttgcataa aactgggttg ttgtttgttg atttatacgg ttttggtgga
      721 tgcaggtttg tcctcaaaca gaatgcggga ggagggagag aaatcatctt caaactggcc
      781 ggactttacg ccccatagaa aagcaaccag aaagatggct gcaacagaaa tactggcaat
      841 cattaagatg ataattacac tcatgtttag ttcaattagg ggtaaaaata ttttgtcacg
      901 acagtgtaat ggttaaccac tatcaacagg cagactgatt ttaatcagtc aaacccattg
      961 attcaggagt ttgtgtcgta tttacagttt caatcgtttg gcaatcaagc tactactgcc
     1021 aaaagtcagt aacaggatac tcagggaact gctgggcatt aatatagccg caatcaacgg
     1081 cgataatgtg ccttgtaccg caaagaagag tccgattaca ttatagacta tcgacaggac
     1141 aaaactggcc accacaatat tccggttggc ccggcagagc cggataaacc gggtaagccc
     1201 ggacagtttc ttcgcttcga taatggcatc actggaaggg gtgaaattgt tggtttgttc
     1261 agctacggct attcctatat cactctgttt caaggctccg gcgtcattga ggccatcccc
     1321 gatcatcatt actttctccc ctttttcctg caaaaaccgg atataattca acttgtcctc
     1381 tggtttctgg tgaaaaagca gggtggcatt ggagcccatc agcttttgca ggttttcctg
     1441 ttcagccata ttatcaccgg ataaaacaga tagcttatac tcttttttca gctcccggat
     1501 cagttcagga atcgcttccc ggtaatggtt actgaaccgg aattgaccaa atacttcatc
     1561 gccaaaccgt acaaatacac tggttccgga agtatgtccg gtattgcctg ttgtaaatac
     1621 ataagatcca agtttaaggg aatcttctcc aaactgcccc tcaattccct gacccggtat
     1681 ttctttaaaa tgatcaatat caaatccctg ctttctgccg atatgccggg ccagcgcctt
     1741 actcaatgga tgattggaat gatcggccag tgcggctacc tgttgcaact gcaattcact
     1801 caattctttt ccttcgtaaa cgatgtcttg ttcctgtgaa gacgtgagtg tgccggtctt
     1861 atcaaatacg atatgcgtag cttttcccag gtcttcaatg gtctgggcat tccgcaggta
     1921 taaatgatta cgtcccagga tccggaggat gtttccattg gtaaatgtgt tggagagaaa
     1981 cagggcacag ggacaggcaa tgataaaaat ggaagtcata gccggccata ccctggacgg
     2041 atcgttgaac tgccagtaaa taaccgtagc agcggcaatc gccagcacga tataggtaaa
     2101 ataccggctg agcaggtgta caaatgattt ggtattgatc tcctcctgct ctttgaattc
     2161 atcccggttc cagagtttgg tcaggtaact ctgcgtcact tctttcacca ccaggatctc
     2221 gatgtttcct tcggtctgtt tacctccggc atatacgatt tcccctatct ctttttgtac
     2281 aggcagcgat tcgccggtta caaaactata atcaataaaa gccttacccc ttgtcagcaa
     2341 gccatcagcg ggaatcagct cttcattatg gatcagcagg gtgtcaccgg gtttgatatc
     2401 cggtaaggca gttggcgtct ctttcccctc ttttaataca gagattgcga tcgggaaata
     2461 agaagtataa tctcgttcaa aggataactg gcggtaggtt ttgtcctgca ggatccggcc
     2521 ggccaacata aagaaaacga tgcccgtcat ggaatcaaaa taaccaccac cggtcccgct
     2581 gatgacctca taggcgctcc ggataaaggt gatgattacc gccagggcaa tgggggcatc
     2641 tatattgaga aacttatgct tcaggctttt ccaggaagaa atataaaagg gtaaggccga
     2701 atacaataat acagggatcg ccagtccgaa attggcccag cggaaaatgg tgagcaatcc
     2761 tttttcgctg gcatcaatac ccaggtactc cgggaagctc atcagcataa tattaccaaa
     2821 ggcaaagccg gccaccccga tctggtaaac catacttctg tcgatagccg gccttttttc
     2881 tttgaggtca ttcaggctga tataaggttc ataaccgata ttggtcaggg tttctgccac
     2941 gctccgcaga ctggttttat ccttgaggaa gacgatatcg gcttcttttc gtgtaaaatt
     3001 gactttactg gacacaaccc ccgggtttag ccggtgcagg ttttcgagca ggtaaagaca
     3061 ggaactgcag tgcatctggg gcaggtaaaa agtgatatgg acctgctttt catcttcaaa
     3121 actgatcagt tgtaattgta ttttctcatc atccagaaag gcgaacttgt catgccgtac
     3181 ccgcacccgc tgattgattc ccggattgtc gttaaggttg taatactcac agagttcgct
     3241 ctggttaagg atctggtaaa ccattttgca accatcacaa caaaacactt tttcagccat
     3301 cctgatcttt gtattcaggc aggtttcccc acagtggtag caatgaattt cctgttcagt
     3361 tataacggag gaggacataa taggtgaaaa aatgttgaag ttgctaaaat agccgcagac
     3421 cccggagcag ggcctgagaa agttcacccg gcaaactgat ttttatcaaa acgttggttt
     3481 tgcctatttt gcaccagttt tagcaacaaa aaaaccaacc gcactatgcc gcaaagaacg
     3541 attctgatta tcgatgacaa tgaagatatc cgggagaata ctgctgaaat cctctccctt
     3601 ggggggtaca agaccctgac ggccgaaaac gggaaaagag gggtggaagc tgctttactg
     3661 gaaaagccag acctgattgt ctgtgatatt atgatgccgg aactggatgg ttatggggta
     3721 cttcacctgc ttcggaagaa tccggaaacc gaaaatattc ccctgatctt cctgacagcc
     3781 aaagcagaac gcagtgatct ccgcaaggga atggagatgg gtgctgatga ttatatcacg
     3841 aagccctttg aagagattga gctgatgaat gccatcgaat cccggcttaa gaaatatgaa
     3901 gtactccaga aaaattacaa tgcaagcgga aaaggcctga gcgaactggc caatgatctc
     3961 cgggaatccg ggatgctcca gttcaacccc gacaattata attctgaatt atataccaaa
     4021 aagcaggtga tatatgccga gggcaaacgc ccccgtttcc tgtattttct ggtaaaagga
     4081 aaagtaaagg gcttcaaaac acatgaagac gggaaggaat atatcaccga tctgtacagt
     4141 gatggtgatt ttatcggata ccccgccctg attgaagaga agaattatga tgattcggca
     4201 gtagccctcg aggatacgga gattgttcag gtgccccgcg aagatttcca gcaaatgatt
     4261 gacggaaata tttcagtggc atccaagttt atccgcatca tcacccagaa tgtaaaggaa
     4321 aaagaagagc gtctgctgag cctggcctat agttcacttc gtaaaagggt agcaaaggcc
     4381 ctggtggata tccatggaaa gttcaattcc gcgggcgaaa acaaacccat cgagatttcc
     4441 agggaggata tcgctcacta tgtaggaacg gccactgaat cgctgatccg taccctgagt
     4501 gatttcaaat cggaaaaact gattgagata aaggatggta aaatcagtat cagcaatata
     4561 gagaaactga agaacctgtt atattaatta cagggataaa agcatttata aaaatgaggc
     4621 tgtccggatc agggcagcct ctccttttca gacacatcct gtcaatttac agagatgctc
     4681 ttcacttttt tgggttgatt gatttccagt ttgggaatca atattttcag ttccccgttg
     4741 ttgtattcgg ccgcaatttt cgcggtatcg cagttttcag gaagggtaaa acttctggac
     4801 cagctgctgt agttgtattc cctgcggttg aaacgtccgt tcttttcttc tttctcttct
     4861 ttttcttttt cagcgctgat ggtcagcatt tcatcaaagg cttccacctt aaaatctttt
     4921 ttctccatgc cgggagcagc tacacaaagc ctgaattcct tatcggtttc ggaaacattc
     4981 acagccggta cattcatgac cttacccatg ttgaaaaatt catcaatcgg ggcgttgaag
     5041 aatttgtcca tccagccact gtcaaacaaa gtgggccagg aagacctggg cttgtcaagt
     5101 gctttgcttt ccatattgat tttttagggt gaataatatt gttgacaccc caatatcaag
     5161 ttcagcgatc aggaaaaaca atgatgaata tgaatttgat caatgacttt aatcattggt
     5221 tgctgctggt taaatgagta tattatatgt ttgtgtacga aggacgatta catgtctttc
     5281 cgcttaaaac tccgcaatgc cagccaaagg ggcacaatac cccagagagc cataatgccg
     5341 gcggtaaaaa gcaccccgct gccactgcca aaaaagtcct tgtacaaagc gcctgtatag
     5401 cccatcagtg cactgatatc cattttcagc atgatataga tccggcccag gtcaatggga
     5461 ttgagggcgg ataagagtaa agtaaatttt tccatcggat aatcactgaa agcaaagagg
     5521 gtcagcagta ccagcccgtc ataaatcaga gcaaaataaa accataacaa aagtgcgaag
     5581 ccaattcccc gggctttatc ccgggaacgt acagctgcca gaaaggcaat gctggtgaat
     5641 acaaccgtta aggaagtgcc ggtaaataac agcgcaaaac cagtggcatc gggtgcataa
     5701 agtattacgg gtactcccac gccgatccag aatgaagata agagggaaac acaaacgccc
     5761 gcatattcac tcagcaggat acgggtccgg ctcaggggtt gtgaaagcat cagctcaata
     5821 aattcatacg aattataata gt
//
LOCUS       DAOPMW010000202         5818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_15889_length_5818_cov_7.067673, whole genome shotgun sequence.
ACCESSION   DAOPMW010000202 DAOPMW010000000
VERSION     DAOPMW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_5818_cov_7.067673"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLU11_09945"
     CDS             complement(<1..180)
                     /locus_tag="PLU11_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59487.1"
                     /translation="MKKIVSILLLASTTVTLSAQTGHVMQGVGAHNMSMGGASTGQVL
                     DINGALQWNPAGITAF"
     gene            521..955
                     /locus_tag="PLU11_09950"
     CDS             521..955
                     /locus_tag="PLU11_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59488.1"
                     /translation="MKQEYWINVKHVDNRLVIYLNGETVWDSGIVHDDPALNKFIEIT
                     SNLDEHPGHTSELIFEGFNDDYTSSGDEGALNPWHFSYRVFKRTVNGNGEVVGESELV
                     NPYNEKHLSNPNTRAINNSYQLVLKSGEYKVVSNALSQQFYK"
     gene            1146..2069
                     /locus_tag="PLU11_09955"
     CDS             1146..2069
                     /locus_tag="PLU11_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN59489.1"
                     /translation="MQTILGANGTIGSLLARELAVYTNRIRLVSRNPVAVNPSDELFP
                     ADLSDPDIVEKAIAGSEVVYLLVGFDYKLTVWQEKWPRLMRSVIDACIRHQAKLVFFD
                     NVYLYDVGAIGHMTEESPINPPSQKGAVRSRIAGMLMEEVKAGKLQSLIARSADFYGP
                     RNEKSFLIEVVYKNFRKGKKANWFMNADKIHSFTYTPDAAKATALLGNTADAWNQVWH
                     LPTDPARITGRQMISLFAREMKQPDKVMVMPLWLLRVLGLFMPLMREMPEMMYQYDRD
                     YFFDSAKFQQRFGIQPTTYENGVKATIEAGI"
     gene            complement(2137..2427)
                     /locus_tag="PLU11_09960"
     CDS             complement(2137..2427)
                     /locus_tag="PLU11_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HPN59490.1"
                     /translation="MVTKTYYKTKDYCKVKFSFQVENAETIEILGLNSNWEESVIMSR
                     KKDGTFSADVNLPKDSKHEFKYLVNEKEWINDPEADSQSPNIYGGTNSVVVI"
     gene            2673..2924
                     /locus_tag="PLU11_09965"
     CDS             2673..2924
                     /locus_tag="PLU11_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59491.1"
                     /translation="MNYLKFNDQAFIAESFNMDEDFTFQSARLAFFKINSYKLSLINA
                     SFCVTRDELKSSISKSLLNYTSPDMVLLTTGFFNQELDN"
     gene            complement(3152..4597)
                     /locus_tag="PLU11_09970"
     CDS             complement(3152..4597)
                     /locus_tag="PLU11_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59492.1"
                     /translation="MEFTAQPLLQQKETATRYERRFDFQRKQILTLFNLIQSFGVTAA
                     MEEYDQRKLRIFNQVNFFQLLIGLLFPIAGMLMSRGLPASAWLVACLPALVSLLVLFL
                     NYTKRQEMSLVAYFILYPFFTCVVYLYGFNPGISLSFILFGILAVFFLNSLGNIIFSL
                     SFSMISYFLLAVVIRHQPFELREISNGLYLMNQGLSLLFIFYGLFLIKKENSIYQGRI
                     LTKNTILEDKNTQIQKQSRKLEESSTLLKQQATQLAELNSVKTKLFSIISHDLKAPMY
                     AIRNLFKEAHEQKLSAEDLKNNIPEVLNDMNYAVGLMENLLQWAKAQMQAHTVNPEVV
                     DIEDSINEVYQALLLPARNKGVILLNDAPPKVFGYCDKDMMSLVLRNLLSNAIKFTPE
                     KGKICIGVHEHPTFLEIYVQDSGMGMSHEALTRIRNNDYFTTKGTASESGTGLGLMLC
                     KEFLVRNGGQLYIESEPGEGSTFSFSVPKPA"
     gene            5083..>5818
                     /locus_tag="PLU11_09975"
     CDS             5083..>5818
                     /locus_tag="PLU11_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPN59493.1"
                     /translation="MAEHSHQLAAILFADIVGYTAMMQENEEDSVEKVNRFRESVELI
                     AEELNGKIIQYYGDGSLLLFQSSTEAAEFAKLLQEDLYNEPRIPVRIGIHMGDVLVHS
                     GNIFGDVVNIASRIQALAPVGGVYISEMVYRNIANKKELEALFVREEKLKNVKGSIRI
                     YQLLTEGAEAYVQPSGVPDTPVAASGPSNIAVLPFANLSSDPQQDYFSDGLTEDIITQ
                     LSKIRAYKVISRTSVMQYKHSPKSVKE"
BASE COUNT         1647 a         1236 c         1363 g         1572 t
ORIGIN      
        1 aaaggcggta ataccagcag ggttccactg taaggcccca ttgatatcca gcacctggcc
       61 ggtggaagca cctcccattg acatattgtg ggcacctacg ccctgcatga catgcccggt
      121 ctgggcagac agagtaacag ttgtacttgc aagtagcaaa attgatacaa tctttttcat
      181 atagtggtat ttaaatgaag cggcaatccg catataaaag taataaaact acctgaaggc
      241 tgtagtggca tatgtgactg ctatcacatc cggggagaaa tcatataggg atatgatgta
      301 gaacaggaaa agggcggatc ggtaaccggg aaaaacggat tttccctgtg aagttagaga
      361 atcacgcagc gggaaaatcc ggggaaagat ggtggaccat cgattaacaa gtatcaggtt
      421 ggcggataac ttttgtcatg gaactgaaac ggaacttatt tctattttcg ttccggaatt
      481 tttttttaac ccattcgtta aagcctaata ttttagcaac atgaaacaag aatactggat
      541 caacgtaaaa catgtggaca atcgcctcgt tatttatctc aatggagaaa cggtttggga
      601 cagtggcatc gtgcatgatg accctgccct gaataaattt attgaaatca ccagcaacct
      661 ggatgagcat cctggtcata ccagcgaact gatttttgaa gggttcaatg atgattatac
      721 ttccagtggt gatgaagggg ccctgaaccc ctggcatttc agttaccgcg tattcaaaag
      781 aacggttaat ggaaacgggg aagtggtagg tgaatcagaa ctggtgaacc cttacaatga
      841 gaaacacctc tccaatccca atacccgggc gatcaataac agttaccaac tggtattgaa
      901 aagcggggaa tataaagtgg tgtccaatgc actgtcccag cagttctata aataagtgaa
      961 cgttaatttt taatatccaa cctgttctca ggttgctctg attatcgcaa gggaggtatc
     1021 cgtgaacaga tgccgccctt tttttatgcc cggttttcag cctgtttacc cgttattacc
     1081 tgccattcac cgaatgacca ctgaagggta cttctgtatt tggtactttg attaaaattt
     1141 aatttatgca gacaatttta ggagccaatg gtactatcgg ttcattgctg gccagagagc
     1201 tggctgtata taccaaccgg atacgcctgg tcagccggaa cccggttgcc gtgaatcctt
     1261 cggatgaact cttcccggcc gatctgagtg atccggatat agtggagaaa gcaatagccg
     1321 gatctgaagt ggtttacctg ctggttggct ttgattataa attgacggtc tggcaggaaa
     1381 aatggcccag gctgatgcgt tcggtcattg acgcgtgtat ccggcatcag gctaaacttg
     1441 ttttctttga taatgtttac ttgtatgatg tgggggcaat cggacatatg acagaagaga
     1501 gtcccatcaa cccgccgagc caaaaaggag ccgtcagaag caggattgca ggtatgctga
     1561 tggaggaggt gaaagcgggg aaactgcaga gcctgattgc ccgttcagcc gatttttacg
     1621 gaccccggaa tgagaagagt tttctgattg aagtcgtgta taaaaatttc aggaaaggaa
     1681 agaaagcgaa ctggttcatg aatgcggata aaatccactc ttttacctat actcctgatg
     1741 cggcaaaagc cacggcactg ttggggaata cagcggatgc atggaaccag gtatggcatc
     1801 ttccaacaga tccggcccgg attaccggcc ggcagatgat ctcccttttt gcccgtgaga
     1861 tgaaacagcc ggataaggta atggtgatgc ccctgtggtt gttgcgggtg cttggtttat
     1921 ttatgccact gatgcgggag atgcccgaaa tgatgtacca gtatgaccgg gattatttct
     1981 ttgattcggc aaagtttcag caacggttcg gtatccaacc caccacttat gaaaatgggg
     2041 taaaagcaac gattgaagca gggatatagc aatacagtga cccgccatca aaggcagatc
     2101 actgtatgct gattatccag tgtacggatg aacgaatcag attacaacga cactgtttgt
     2161 gccgccataa atattggggg actggctgtc tgcttcagga tcattgatcc attctttttc
     2221 attgaccagg tacttgaatt catgtttaga atctttaggc agatttacat ccgcactgaa
     2281 ggttccatct tttttacggc tcataataac tgattcttcc cagttactgt tgagccccag
     2341 aatttcaatt gtttctgcgt tttctacctg aaaagaaaat ttcaccttgc aataatcctt
     2401 tgtcttgtaa taggtcttag ttaccatttg ccgttttttg gggttaaata atatgcgtaa
     2461 ttcattgaca aacagcaatc ggggagtttt ctggagggtg caagtatccg tctttaatac
     2521 ccgaaagggc aaaaggtaac ccggctggat ccaggcgctg ttaacaggtt atccacgtca
     2581 ggaaaacggg attataaaac caggaataca attttccgtt aaatcagtcg tttagaggat
     2641 aatccaatcg aagaaaaact aatatccgtc gtatgaacta cctgaaattt aatgatcagg
     2701 catttattgc cgagtcattc aacatggacg aagattttac gttccagtca gccaggctgg
     2761 cctttttcaa aattaattcc tacaaacttt cactgattaa cgcctctttc tgtgtcaccc
     2821 gtgacgaact gaagagcagc atcagcaaga gcctgctcaa ttatacgagt ccggatatgg
     2881 tgttgctgac aaccggtttt ttcaaccagg aattagacaa ttagcataac tctgcgggat
     2941 tggataccct gcgatttcat atgcctgcga accgggcatg ggaaagctgt ctcataacga
     3001 gatggctttt ttagtttagg taattgatcg gtgatagggg tagaagtcag ttgtcagttg
     3061 tcaggtgtca gatgtcagga aacagcccca cctcacagat aaatgttcaa ggcacgtcag
     3121 tcgtacttcg tccctcttac ttcgtacttt atcaggcggg ttttggtact gaaaacgaga
     3181 acgtgcttcc ttctccgggc tcactttcga tgtaaagctg tccgccgttt ctcacgagga
     3241 attctttgca gagcatcagt cccagtccgg taccggattc gctggccgta cccttggtgg
     3301 tgaagtagtc gttgttacgg atacgcgtca gggcttcatg actcatcccc ataccactgt
     3361 cctgcacata tatttcaagg aaggtcgggt gttcgtgtac cccgatacag atcttccctt
     3421 tttcgggagt gaatttgatg gcattggaga gcaggttccg cagcacgaga ctcatcatat
     3481 ctttatcaca ataaccaaat accttgggag gggcatcatt gagtaagatc actcctttat
     3541 tacgggctgg caggaggagg gcctggtata cttcattgat tgaatcctct atatccacca
     3601 cctcgggatt aacggtatgc gcctgcatct gggctttggc ccactgcagc aggttctcca
     3661 tcagtcctac cgcataattc atatcattga gtacttccgg tatattgttt ttcaggtctt
     3721 ccgcagaaag tttttgctca tgcgcttctt taaacaggtt gcggattgca tacatcggcg
     3781 ctttcaggtc atgcgaaatg atgctgaata atttggtctt tacggaattc aattcagcga
     3841 gctgggtggc ctgttgcttt agcagggtgg aactttcttc cagcttacgg ctttgcttct
     3901 gaatctgcgt gttcttatcc tccaaaatgg tgttcttggt aaggatacgt ccctggtaaa
     3961 tactgttctc tttttttatc aggaataagc catagaatat aaatagtaag gaaaggccct
     4021 ggttcatcag gtataagcca ttactgattt cacggagttc aaatggctgg tgacggatta
     4081 ccaccgccag caggaagtag ctgatcatgg aaaaactcag ggagaaaata atattaccca
     4141 gtgaattcag gaagaaaacg gccagtatcc caaacaggat aaaggaaagg ctgatgcccg
     4201 gattaaaacc atagagatac accacacagg tgaaaaacgg gtaaaggata aaataagcca
     4261 ccaatgacat ttcctgtctt ttcgtgtagt tcaggaaaag aaccagcaaa ctcaccaatg
     4321 cgggcagaca ggccaccagc caggcagagg cgggtaatcc ccggctcatc agcataccgg
     4381 caatcgggaa gagcagaccg atcagcagct gaaagaaatt tacctggtta aaaatgcgca
     4441 gctttcgctg atcatattct tccatagccg cagtcacccc aaatgactgg atgagattaa
     4501 aaagggtcag gatctgtttc cgctgaaaat caaaacggcg ctcataccgt gttgcagttt
     4561 ctttctgctg gagtagtggc tgtgcagtaa attccattgg tttaacgctt aagtattgga
     4621 taaacaaaat aacaggtaat gcgaacaatt acctacaaat caacgagaaa cttacttcca
     4681 aatcgtataa ttacgtatcc aggactgcgg aaaactgcat aatcaaaaca gggtagaact
     4741 aaccgataat cctgaaaata gcgaaatgag catacgtgat gtcttctaaa accgaccggc
     4801 aactggccgg tcacagtttt ttgcataaaa aaacaatccg gatagggctg atcctattac
     4861 ttcttgcttt tatgtgttaa atatttattg aatgagaaca gtttattcag gttgattgca
     4921 atttattttc ttaataagga ataagggtat aacagtaaat gagatcaatt tttgcactgg
     4981 ggtataccgg aattttgaaa atttaaagga aattagtttt cataggtgaa ccacttctga
     5041 aaagagtatt gcttttacta actttgcgca gacttcaacc aaatggcaga acattcacat
     5101 cagctggcag caatcctttt tgctgatata gtcggttata cggctatgat gcaggaaaac
     5161 gaggaggact ccgtagaaaa agtgaaccgt ttcagagaat cggtcgaact gatcgccgag
     5221 gaactcaacg gtaaaatcat ccaatattac ggggatggtt ccttattact atttcagagc
     5281 tctactgaag ccgcggaatt tgccaaatta ctccaggaag acctgtataa tgaaccccgg
     5341 attccggtcc gtattggcat tcatatgggt gatgtcctcg tacatagcgg aaatattttc
     5401 ggggatgtgg tcaatattgc ctcccggata caggccctgg ctccagtagg gggtgtctat
     5461 atttctgaaa tggtctatcg caatattgcc aataaaaagg agctggaagc cttatttgtc
     5521 agggaagaaa agctgaaaaa tgtgaaagga tcgatacgca tttaccagct gcttaccgaa
     5581 ggggcagaag cctatgttca gccatccggc gtccccgata cgccggttgc cgcttccgga
     5641 cccagtaata ttgccgttct cccgtttgca aacctgagca gcgatccgca gcaggattat
     5701 ttcagcgatg gacttacgga agatatcata acccagctgt ccaaaatccg ggcctacaaa
     5761 gtcatttccc ggacttcggt catgcaatac aagcattcac ctaaaagtgt aaaagaaa
//
LOCUS       DAOPMW010000203         5770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16064_length_5770_cov_4.761680, whole genome shotgun sequence.
ACCESSION   DAOPMW010000203 DAOPMW010000000
VERSION     DAOPMW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16064_length_5770_cov_4.761680"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /gene="bamD"
                     /locus_tag="PLU11_09980"
     CDS             <1..689
                     /gene="bamD"
                     /locus_tag="PLU11_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPN59494.1"
                     /translation="QYVKAQQVYEDIMPYYKTTPEFQDIYYKYAYCAYNLEDYMNAEN
                     LFNSFLEIFPNSPMAEEMDYMRAYSFYKQSPKAELDQTNTIRAMGMLQTFINTHPGSA
                     RNKEANEIIDLCRAKLEIKDFKSAQLYYDLGQFRAAGVAFSALLNDYPESMKGDEYKL
                     MIIKSYYKYAEMSVEEKKTERFEQVISHCYEFTDRFPDSKLKPEADNYLNLSQSNIKN
                     LNNEQIKTPA"
     gene            664..1005
                     /locus_tag="PLU11_09985"
     CDS             664..1005
                     /locus_tag="PLU11_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPN59495.1"
                     /translation="MSKLRRQLSAGITNNVETRNLDDIKAKTGNLYESIAIIGKRSSQ
                     INISLKEELHNKLEEFASHTDSLEEIHENKEQIEISRAYERMPNPALLATQEFMEDKV
                     YYRKGEDELFS"
     gene            1118..2317
                     /gene="coaBC"
                     /locus_tag="PLU11_09990"
     CDS             1118..2317
                     /gene="coaBC"
                     /locus_tag="PLU11_09990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPN59496.1"
                     /translation="MLKGKKIMLGITGSIAAYKAVVLVRELVKAGAEVRVIMTPAAKD
                     FVTPLTLSTLSRNPVLTDLFDEQSWSNHVMLGRWADVFLVAPMSCNTLAKMANGICDN
                     LLMAVYLSATCPVVLAPAMDEDMWQHPSTRINLTRLEEFGNKIIPVGTGALASGLNGE
                     GRMAEPEQILQFLGEQVFKKGALQGKKALVTAGPTYEPIDPVRFIGNHSSGKMGMALA
                     RELQARGAEVTLVLGPVGDEPELPGINIVSVMTAAQMYTVCLQVFPASDMAIMAAAVA
                     DYTPVQTAVEKIKKTGESLPLELKKTRDILKSLGEIKTKDQVLVGFALETTEEKKNAL
                     AKLETKNADIIVLNSLNDAGAGFGKDTNKVTLFTRDGKEFPFDTKSKQAVAADITDTI
                     IQLYYAK"
     gene            2307..3221
                     /locus_tag="PLU11_09995"
     CDS             2307..3221
                     /locus_tag="PLU11_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPN59497.1"
                     /translation="MRSRIILVPFLLLLVMNSSAQEIQARLSVIASKVSTQIDRKIFN
                     TLQTGLTNFLNARKWTNDVFQPSEKIQCSFLITIEKDLGNNVFEGKMTIQAARPAFNT
                     TYDCPMLNFQDDNIIFKYQEFQPIEFNENRVQGNDPLVGNLTAIMAYYVNIILGFDYD
                     SYSLRGGDLYFQKAWNIVNNAPEGREITGWKSFESLRNRYWLAENMNNSRFALMHDVL
                     YSYYRSGVDVFFENEEAGRSGIINALNFLNTLNTENPNSMVMQLFFQGKANELVKIFS
                     KAGGEQKSRAKELLTRLDVTNATQYKAL"
     gene            3221..3664
                     /locus_tag="PLU11_10000"
     CDS             3221..3664
                     /locus_tag="PLU11_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPN59498.1"
                     /translation="MRKCLLLFIIFFAVACNSKTDAPAKLLSRDKMEDILWDLMRADL
                     FINNYMLIKDTALDKKKQGIELYSQILKLHKVSQEQFRESFIYYRSQPEELKVLMDSL
                     SHHSDSAQLQKSAKTPTADSLPEQVVIPEKKDTGKKKIIPVRKIE"
     gene            3720..4421
                     /locus_tag="PLU11_10005"
     CDS             3720..4421
                     /locus_tag="PLU11_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPN59499.1"
                     /translation="MNKVVSGAEEAIRDIADGATLMLGGFGLCGIPENCIDALVKKGI
                     SQLTCISNNAGVDDFGIGLMLKQKQVRKMISSYVGENAEFERQLLSGELEVELIPQGT
                     LATRCLAAGYGMPAVFTPAGVGTEVADGKESRVFNGKEYLMEMAFEADFAMVKAWKGD
                     TQGNLVYRETARNFNPLMAMAGKITIAEVEELVPAGTLDPDHIHTPGIYVHRIFKGNA
                     YQKRIEQRTVRNKGE"
     gene            4455..5111
                     /locus_tag="PLU11_10010"
     CDS             4455..5111
                     /locus_tag="PLU11_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPN59500.1"
                     /translation="MALNKEQIAQRIARELQDGFYVNLGIGIPTLVANYIPPGMDVVL
                     QSENGLLGMGPFPFEGEEDPDLINAGKQTITTVPGSVFFDSAMSFGMIRAGKVDLTVL
                     GAMEVSEKGDIANWKIPGKMVKGMGGAMDLVASARNIIVAMMHTNPKGESKLLPACSL
                     PLTGVGCVKKIVSDLAVLDITPQGFKLLERAPGVSVEEIKAKTAGKLIVEGDIPEISF
                     "
     gene            complement(5124..5765)
                     /locus_tag="PLU11_10015"
     CDS             complement(5124..5765)
                     /locus_tag="PLU11_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59501.1"
                     /translation="MKVAIADDHKIFRKGVILSLRPYSNIKFVQEAENGEDLLNGLPA
                     SEPDVILMDLRMPGKDGIEATKAVSKQYPQIKVLILSMYEDERFVYHLMENGANGYLL
                     KSAEPQEIRRAIMEVHEKGYYLNNFVNRILLKKSHSRQKVVPSLNSEITLSDKEKDVL
                     RFICMEFTAAEIAQKMEISPRTVEAIKDRLMERFGSKNTAGLVFFAVKNNLIE"
BASE COUNT         1639 a         1229 c         1468 g         1434 t
ORIGIN      
        1 agcaatatgt aaaagcccag caggtatatg aggatatcat gccgtattat aaaacaacgc
       61 cggaattcca ggatatctat tataagtacg cctattgtgc ttataacctc gaagactata
      121 tgaatgcgga gaacctgttt aacagttttc ttgagatatt cccgaacagc cccatggcag
      181 aagaaatgga ttatatgcgg gcctattcgt tttataagca atcgccaaaa gccgaactgg
      241 accagaccaa tacgataaga gccatgggga tgctgcagac ctttatcaat acacacccgg
      301 ggtcagcacg gaataaagag gccaatgaaa tcattgacct ttgccgggca aaactcgaaa
      361 taaaggattt taaaagcgcc cagttatact atgatctcgg acagttcagg gccgccggtg
      421 tggccttcag cgccttgctg aatgattacc cggagtcaat gaaaggtgat gagtataaac
      481 tgatgatcat taagtcgtat tataagtacg ccgaaatgag tgtggaggaa aagaaaacag
      541 agcgttttga acaggtgatc agtcattgct atgagtttac ggacagattt cccgacagta
      601 aactgaagcc ggaagccgat aattacttaa atttatcaca gtcaaatatt aaaaatctga
      661 ataatgagca aattaagacg ccagcttagc gcaggtatca ccaacaatgt ggaaacccgc
      721 aatcttgatg acatcaaagc caagaccggc aatctgtatg aatcaattgc cattattggt
      781 aaaagatcca gccagatcaa tatctcgctg aaagaagaac tgcacaataa actggaagaa
      841 tttgccagtc ataccgacag cctggaagag atccacgaaa acaaagaaca gatcgagatc
      901 tcccgtgctt atgaaagaat gccgaacccg gccctgctgg caacacagga attcatggaa
      961 gacaaagtgt attaccgtaa aggagaagat gagctgttca gctgatcttt tgtaactttt
     1021 tatagcaagc ggcagggttc ctgccgcttt tttatttaac agctatttac cgccacagtt
     1081 atgtcctttt ataaggaagt ggtatttttg atcagctatg ttgaaaggta agaaaatcat
     1141 gttgggaatt acgggtagca ttgccgctta taaggccgtg gtgctggttc gtgaactggt
     1201 taaagccggg gcagaagtcc gggtgatcat gacgcctgcc gccaaagatt ttgtgacccc
     1261 cctgactctt tccaccctca gccggaatcc ggtactgacc gatctgtttg atgaacaaag
     1321 ctggtccaac catgtaatgc tgggccgctg ggctgatgta tttctggtag cacctatgag
     1381 ttgtaatacc ctggccaaaa tggccaatgg catttgcgat aacctgctga tggctgtata
     1441 tctctcggcc acctgcccgg tagtgttggc cccggctatg gacgaggata tgtggcagca
     1501 tccttccacc cggattaatc ttacccggct ggaagaattt ggaaacaaaa ttattcccgt
     1561 cggtaccggg gcgttggcca gcggactcaa cggggaagga cgaatggctg agcccgagca
     1621 aatactgcag ttcctcgggg agcaggtttt taaaaaaggg gctttacagg gcaaaaaagc
     1681 cctggtcact gccgggccca catacgaacc aattgatccg gttcgtttta tcggtaatca
     1741 ctcttcagga aagatgggaa tggccctggc gcgcgaattg caggccaggg gagctgaagt
     1801 aacccttgta ctgggacctg tgggggatga accggagttg cctggaataa atatcgtatc
     1861 agtaatgacg gctgcacaaa tgtatacagt ctgtctgcag gtttttcctg catcagatat
     1921 ggccattatg gcggcggcgg tggcggatta tacaccggtg caaacagctg ttgaaaagat
     1981 taagaaaacc ggtgaatcgc ttcccctgga actgaaaaag acccgggata ttctgaagag
     2041 cctgggtgaa ataaaaacaa aagaccaggt gctggtgggc tttgccctcg aaaccactga
     2101 ggagaaaaaa aatgcgttgg ccaaactgga aacgaagaat gccgatatca tcgtgttgaa
     2161 ttcgctcaat gatgccggcg ctggttttgg aaaggatacc aataaagtaa ccctgtttac
     2221 ccgtgacggt aaggagtttc cttttgatac aaaatccaag caggcggtgg ctgctgatat
     2281 tacggacaca ataatccaat tgtattatgc gaagtagaat tatacttgtt cctttcctgt
     2341 tactgctggt gatgaatagt tccgcgcagg aaattcaggc gcgtctgtca gtcattgcca
     2401 gtaaagtaag tacgcaaatt gaccggaaga tttttaatac cctgcaaacc ggactgacta
     2461 atttcctgaa tgcccggaaa tggaccaatg atgtgtttca accttcggaa aaaatccaat
     2521 gcagtttcct gatcaccatt gaaaaagatc tgggcaataa tgtctttgaa ggcaaaatga
     2581 ctatccaggc ggcccgtccg gcttttaata ccacgtacga ttgtccgatg ctgaacttcc
     2641 aggacgacaa tataattttc aaataccagg aattccagcc gattgaattt aatgagaacc
     2701 gggtgcaggg aaatgatccg ttggtgggaa acctgaccgc aataatggct tattatgtca
     2761 atatcatact gggatttgat tatgattcct attctcttcg gggaggggat ctttatttcc
     2821 agaaagcatg gaatatagtc aacaatgccc cggaaggccg ggaaatcacc ggatggaaat
     2881 cttttgaaag cctgcgtaac cgctactggc tggcggagaa tatgaataac agccgctttg
     2941 cgctgatgca tgatgtgctg tattcttatt accgatccgg cgtggatgtc ttttttgaaa
     3001 acgaggaagc cggccggagc gggatcatca atgcactcaa ttttctcaat accctgaata
     3061 cggagaatcc gaactccatg gtaatgcagc tctttttcca gggcaaggcc aatgaactcg
     3121 tcaaaatttt cagtaaagcc ggaggggaac aaaagagcag ggccaaagaa ctcctgaccc
     3181 gcctggatgt aactaatgca actcagtata aagcactgta atgagaaaat gcctgttgct
     3241 gtttattatt ttttttgcgg ttgcctgtaa tagcaagacg gatgccccgg ctaaattatt
     3301 gtcgcgggat aaaatggagg acattctctg ggacctgatg cgtgcagatt tgtttatcaa
     3361 taattatatg ctgataaagg atacagccct ggataaaaaa aagcagggga ttgaattgta
     3421 cagccagatt ttaaagttgc ataaagtcag ccaggagcaa ttcagggaaa gttttatata
     3481 ctaccgttcc cagccggaag aattgaaagt gctgatggat tccctgagtc atcatagtga
     3541 cagcgcacaa ttacagaagt ccgctaaaac cccaacggca gacagtcttc cggaacaggt
     3601 ggttattcca gagaaaaaag acacgggaaa aaagaaaata atccctgtca ggaaaatcga
     3661 atgaacgttc ggttgcaatt cggctcaagt ccttattttt actgtctaat caagtttaca
     3721 tgaataaagt tgtcagcggg gctgaagaag ccatccgtga tattgctgat ggagccaccc
     3781 tgatgttggg tggatttggt ttatgcggta tcccggaaaa ctgtatcgat gccctggtaa
     3841 aaaaagggat cagtcagctc acctgtattt ccaataatgc aggagtagat gatttcggga
     3901 tcggattgat gctgaaacaa aaacaggtca ggaagatgat atcttcctat gtgggggaga
     3961 atgcggagtt tgagcggcaa ttactcagcg gggaactgga agtggagctc ataccccagg
     4021 gtactttggc tacccgttgc ctggcggcag ggtatggcat gcctgccgtg tttacacctg
     4081 ccggtgtggg tactgaagtc gccgatggta aagaaagccg ggtttttaac gggaaggaat
     4141 atttaatgga aatggccttt gaagctgatt ttgccatggt gaaggcctgg aaaggcgaca
     4201 cgcaagggaa cctggtatac cgggaaaccg ccaggaattt caatcccctg atggccatgg
     4261 cgggaaagat aaccattgca gaagtggaag aactggtacc cgccggcacc ctggatccgg
     4321 accatattca tacacccgga atatatgtac accgcatttt taaaggcaat gcttaccaga
     4381 aacgcattga gcaacgtaca gtacgaaata agggtgaata agaaagtttc tttcattcaa
     4441 caaccaacta atttatggca ctgaataaag aacagattgc gcagcggatt gcccgcgaac
     4501 ttcaggatgg tttttatgtc aacctgggca ttggaattcc aaccctggta gcgaactata
     4561 tacctcccgg aatggatgtg gtattacaaa gtgaaaacgg gttattaggg atggggccat
     4621 ttcctttcga aggagaggag gatccggacc tgatcaatgc cggaaaacaa accatcacta
     4681 cggtgccggg ctctgtcttc tttgattcag cgatgagttt cggcatgatc cgggccggaa
     4741 aagtagacct gaccgtgctg ggcgccatgg aagtgagtga gaagggagat attgccaatt
     4801 ggaagatccc gggtaaaatg gtgaagggca tgggaggcgc gatggattta gtcgccagtg
     4861 cccggaatat cattgtggcg atgatgcata ccaatcccaa aggggaatcg aaattattac
     4921 cagcctgcag tctgccactc accggggtgg gttgtgtgaa aaaaatcgta tcggacctgg
     4981 ctgtactgga tataactccg caagggttta agttactgga acgggcacct ggggtgtcgg
     5041 tagaagaaat aaaggcaaaa acagcaggaa aactgatcgt ggagggtgat attccggaaa
     5101 tctcttttta aggcgatccg ggcttattct atcaggttgt tttttactgc aaagaagacc
     5161 aggccggcgg tattcttgct cccaaaacgc tccataaggc ggtccttgat ggcttccacg
     5221 gtacggggac tgatctccat cttctgggcg atctccgcgg ccgtaaattc catacaaata
     5281 aaacggagta catctttttc cttatcactg agggtgatct cgctattgag ggagggcacc
     5341 actttctgcc gggagtgcga tttcttcagc aggatgcggt tcacaaaatt attcaggtaa
     5401 tagccttttt catggacttc catgatggcc cggcggattt cctgtggttc ggcgcttttc
     5461 aggaggtaac cattcgcccc gttttccatc aggtgataga caaagcgttc atcttcgtac
     5521 atactcagga tcagcacttt tatctggggg tattgcttac tcaccgcctt ggtggcttcg
     5581 atgccgtctt taccaggcat acgcaggtcc atcaggatca catctggctc agaagcaggc
     5641 agcccgttga gcaggtcttc cccgttttct gcttcctgta caaattttat attggaataa
     5701 gggcgcaggg agagaatgac tcctttccgg aagatcttat gatcgtcagc tatcgctacc
     5761 ttgatggttc
//
LOCUS       DAOPMW010000204         5755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16121_length_5755_cov_7.417193, whole genome shotgun sequence.
ACCESSION   DAOPMW010000204 DAOPMW010000000
VERSION     DAOPMW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16121_length_5755_cov_7.417193"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..574
                     /gene="crcB"
                     /locus_tag="PLU11_10020"
     CDS             203..574
                     /gene="crcB"
                     /locus_tag="PLU11_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPN59502.1"
                     /translation="MLNNLLLVGLGGGLGSMARYLCQRWFAEVYPHSFPWGTFAVNIT
                     GCLMIGVIWGVSFRSFDSNEQWKLFLMTGLCGGFTTFSAFTLEGIGLLREQKTALFFA
                     YLAASVLLGLLATYAGMKLTR"
     gene            585..1127
                     /locus_tag="PLU11_10025"
     CDS             585..1127
                     /locus_tag="PLU11_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPN59503.1"
                     /translation="MLTVSHPWHGVNYGKKAPATVNALIEIPQGSRSKYEVDKESGLL
                     KLDRVIYSSFQYPVNYGFIPQTLGLDGDPLDILVLCSQSIRSLCLVEANVIGNMQMID
                     SGQEDDKIIAVAAQDPSVNHFRRIEELPQHFLLELKNFFEQYKVLENKKVEIDTFQDQ
                     GIAFRIIKESIDLYNETYKK"
     gene            1151..1567
                     /locus_tag="PLU11_10030"
     CDS             1151..1567
                     /locus_tag="PLU11_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPN59504.1"
                     /translation="MATQLVITALLIGLSAGVLSGLVGVGGGIIMVPALVFFMHYTQH
                     QAQGTSLAVLTLPVVVLASIYYYYQCQKLGTPIDLKIVGLLAVGFIGGGYFGGKLALS
                     IDQESLKKIFAVILMYTAFKMLHWDMLLVKWVKSIF"
     gene            1564..2187
                     /locus_tag="PLU11_10035"
     CDS             1564..2187
                     /locus_tag="PLU11_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59505.1"
                     /translation="MKPEKFMKFLAVLFLVLSLSTLHAQPEKDRLDSLTKQYFIVNPD
                     GHTIPEFLKELTKSGTFTTDTLIPASKNSLLYWRGYSTKLNPFSFPVSRLEMQIRQTL
                     LRHQDQSIPADTVLVFQLIAQAGADEEYKQWVKKETAFLHQQFAPCIHRFKHSQSKRN
                     GKMPFEQWQGYQEAVTFPVISFGWAGHYTDKSVHTIQLTRYFQTKPE"
     gene            2184..2834
                     /locus_tag="PLU11_10040"
     CDS             2184..2834
                     /locus_tag="PLU11_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59506.1"
                     /translation="MKHKTHLLLLTTLLLTSTLYSQISDEKIMGAAGKIAHDYFRVSP
                     FNKDFSTFVSRLMNDPSVQQSTIRRKTDTTLFFLEGIYSTHKPFFFRPDKVKIILSER
                     ETEGDSINPAQPFFLYQIVGYAPPGKEGKTDVQDEYEKLVRQYKRAFDMNHPRELKDG
                     DTLSGEIRDFYFENVSFPVMTLAWSTNEINGNVLAITIRFFVYDNRAFLPIAAYRF"
     gene            complement(2808..3575)
                     /locus_tag="PLU11_10045"
     CDS             complement(2808..3575)
                     /locus_tag="PLU11_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPN59507.1"
                     /translation="MKYAIVNVPVAALRRKPRHPKEMVNQLLFGETVQVLKTKGDAWV
                     KVKSLHDNYEGWMTLSLLEETDEITALQKPVFVTAGLLTRISQDSQAMHIPIGASLPN
                     FTEGTCMINKQAFFVEGLVLNRMEHPASSVLIDKLVQPWLNAPYLWGGRTPLGVDCSG
                     FVQVNFKLMGIDLPRDAWQQAQYGEPVESINEAKPGDLVFFDDKEEIVHVGILLNKET
                     VIHASGKVRIDPIDKKGIINAQTGKRTYRLKAIRRYW"
     gene            3672..4250
                     /locus_tag="PLU11_10050"
     CDS             3672..4250
                     /locus_tag="PLU11_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN59508.1"
                     /translation="MKADKYAHISDQELLDQFYADHNNEWLGILLQRYTLLLLGVSMK
                     YLKNEEEAKDSVQQIFLKVIQELQKYKVEYFKSWLYMVAKNHCLMRIREKQGKITTEI
                     SERHTATPGEETDRQALIQNDHTLDLMERSLSELNPEQQQCVTLFYLQKKSYQEVSEE
                     TGFSMLQVKSYIQNGKRNLRLLVEKKLKEKGI"
     gene            4360..4722
                     /locus_tag="PLU11_10055"
     CDS             4360..4722
                     /locus_tag="PLU11_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59509.1"
                     /translation="MSENLKDILSNEHPEIDPETMMLYLQGKLTPEQKHEVEKLLMQG
                     DFENEAMEGLQEFKDKVQIQYTIEALNRDLKKKTEKKQKRRDKLKLPDQTWIIISVVI
                     VLLLVIVSYFVIRKLLQN"
     gene            4906..5397
                     /locus_tag="PLU11_10060"
     CDS             4906..5397
                     /locus_tag="PLU11_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59510.1"
                     /translation="MRWEKVSFTPDNEVYHLYKDEQKILTLHFHPFSHSARVETGNEK
                     RVFQIRKEGFLRNKTVLRTEYGIKLGELGHENGRSFINIENERYFYSIINNPLAELQI
                     FKEQAEKPMITCGLKTDEGNPEVRFKRDKNQLDGSYSSLLMALCWYMFLPVAKENTPE
                     MAI"
BASE COUNT         1689 a         1320 c         1235 g         1511 t
ORIGIN      
        1 ctcaaagtga tcaaaaccgg aggcaaatgg ctggtggatt tcaaatacct gtacctgcat
       61 gataccgata cagtgactaa taaaccgaaa acaccttaat cggttgtcag ttgccgggtg
      121 tcgggtgtca ggaacagccc ttactaagca gattcccgtt taaacgtcaa acgcctaaac
      181 aactaaaccc tgataatttc caatgctaaa taatcttctc ttagtgggtc tcggtggtgg
      241 tcttggcagt atggcccgct acttatgcca gcgatggttt gctgaagtgt atccccatag
      301 ttttccctgg ggtacgtttg cagtcaatat caccggctgt ctgatgattg gggtgatctg
      361 gggagtgagt ttccggagtt ttgattcaaa tgaacagtgg aagctttttc tcatgaccgg
      421 actctgcggg ggatttacta ctttttccgc ctttacgctg gaaggaattg gcctgctcag
      481 ggaacaaaaa acagctcttt tctttgccta tttagcagcc agtgtgctgc ttggattgct
      541 ggccacttat gccggcatga aattaacccg ataaactcta aactatgtta acagtcagtc
      601 acccatggca tggcgtcaat tatgggaaaa aagccccggc aaccgtcaat gccctgatcg
      661 aaattccaca gggttcccgt tctaaatatg aagtggataa agaatccggt ctgctgaaac
      721 ttgaccgcgt gatctattcc tccttccaat acccggtcaa ttacgggttc atcccccaga
      781 ccctcggact ggatggggat ccgttggata ttctggtact ctgttcccaa tccatccgct
      841 ccctctgcct ggtggaagcc aacgtgatcg gcaatatgca gatgatcgac agcggacagg
      901 aggatgataa gattattgcc gtagctgccc aggatccatc ggtgaatcac ttccggcgga
      961 tcgaggaatt gccacaacat ttcctgctgg aactaaaaaa cttctttgaa cagtataaag
     1021 tactggagaa taaaaaagtg gagattgata ctttccagga ccagggcatt gccttccgga
     1081 tcatcaagga atccatcgat ctgtacaatg agacttataa aaaatgatgc aaggcaccca
     1141 aaaacaaact atggcaacac aactggtgat caccgccctg ctgatcgggt tgtcggcagg
     1201 cgtactcagc ggactggtcg gcgtgggcgg cggtattatc atggtaccgg cccttgtctt
     1261 ctttatgcat tatacccagc accaggccca gggtacatcc ctggccgtac tgacattacc
     1321 ggttgtagtg ctggcatcga tttattatta ttatcagtgc cagaaactgg gtaccccgat
     1381 agatcttaaa atagtaggac tactggcggt tggttttatc gggggtggct attttggcgg
     1441 taaactggct ttatccatcg atcaggaatc cctgaagaaa atctttgcgg tgatcctgat
     1501 gtacacggca tttaaaatgt tgcattggga tatgctctta gtcaaatggg tcaaatccat
     1561 attttgaaac ctgaaaaatt tatgaaattt ctggccgtat tatttttagt actttctttg
     1621 tcaaccctgc atgcacagcc agaaaaagac agattggatt cacttaccaa acagtatttt
     1681 atagttaatc cagatgggca taccataccc gagtttctga aagaactgac aaaaagcgga
     1741 acatttacta ccgacacact tattccagca agtaaaaaca gccttcttta ctggcgggga
     1801 tattccacaa agctcaaccc gttttccttt ccggtgagcc ggctggaaat gcaaatccgg
     1861 caaacactat tacgacatca ggatcagtca ataccagcgg acacggtgct tgttttccaa
     1921 ttgattgccc aggcaggagc agatgaagag tacaaacaat gggtaaaaaa agaaacagca
     1981 tttctgcatc agcaatttgc cccttgtatc caccggttta aacattcaca atctaaaaga
     2041 aatggtaaaa tgccttttga acaatggcag ggctatcagg aagctgtaac tttcccggtg
     2101 attagttttg gatgggctgg ccattatact gacaaaagtg tacatacaat acagctcacc
     2161 cggtattttc aaaccaaacc ggaatgaaac acaagacaca cctgttgctg cttaccacac
     2221 tgcttctaac cagcaccctc tacagccaga tatcggatga aaaaataatg ggggccgcgg
     2281 gaaaaatcgc ccacgattat ttccgggtca gtcccttcaa taaagacttc agcacttttg
     2341 tatcccggct gatgaatgat ccatccgttc aacagagtac cattcgccgc aaaacagata
     2401 ccaccctgtt ctttctggaa ggaatataca gcacacataa accttttttc ttccggccgg
     2461 ataaagtaaa aatcatcctg tctgaaaggg aaaccgaggg cgactccatc aatccggccc
     2521 aacctttttt cctgtaccag atcgttggtt atgccccgcc cggaaaggaa gggaaaacgg
     2581 atgtacagga tgaatatgaa aaactggtac gtcagtataa gcgcgctttt gatatgaatc
     2641 atccccgtga actgaaagac ggggataccc tttccggaga aatccgtgat ttttattttg
     2701 aaaatgtcag cttcccggtt atgaccctgg cctggagcac caatgagatc aatggcaatg
     2761 tgctggccat caccatccgg ttttttgtat atgataaccg ggctttttta ccaatagcgg
     2821 cgtatcgctt ttaaccggta agttcttttg cctgtctgcg cattaataat tcctttttta
     2881 tcaatcggat caatcctgac cttccccgat gcatggatga ccgtttcttt attcagcagg
     2941 atacccacat gaacgatctc ttctttatca tcaaagaaga ccaggtcgcc cggcttcgct
     3001 tcatttatac tttccaccgg ttctccgtac tgtgcctgtt gccaggcatc ccgtggcagg
     3061 tcgatcccca ttaatttaaa attgacctgg acaaaaccac tgcaatcgac ccccaacggc
     3121 gtccggccac cccataaata aggggcattg agccagggct gaaccagttt atctattaat
     3181 acggaagaag ccggatgttc catacggttc agcaccagtc cttctacaaa aaaagcctgc
     3241 ttgttgatca tacaggtacc ctccgtaaaa tttgggagcg atgcaccaat gggaatatgc
     3301 atggcctggc tatcctggct gatccgggta agcaatccgg ccgtcacaaa aaccggtttc
     3361 tgcaaggcgg taatctcgtc ggtctcctcc agcagactca gggtcatcca gccttcatag
     3421 ttatcgtgca ggcttttgac ttttacccag gcatcccctt tagttttcag tacctgcacc
     3481 gtttccccga aaagcaactg gttcaccatt tccttagggt gcctcggttt ccggcgtaaa
     3541 gcagcaacgg gtacattcac gatggcgtat ttcatgaaaa tctttttccg aaattaagga
     3601 atgttctttc cgaaaatttt gttgaaatac acaaatcccg catcttacat gaagaaatca
     3661 ttatcccagc ggtgaaagcg gacaaatacg cccatatcag cgaccaggaa ctgcttgatc
     3721 agttttatgc cgaccacaat aacgagtggc tgggcatcct gctgcaacgg tataccctgc
     3781 tgctgctggg ggtttccatg aagtacctga aaaatgaaga ggaagccaaa gacagtgtgc
     3841 aacagatctt cctcaaagtg atccaggaac tgcagaaata caaagtggag tatttcaaat
     3901 cctggctcta tatggtggcc aaaaatcatt gcctgatgcg gatccgggaa aaacagggga
     3961 agatcaccac cgagatcagc gaaaggcata cggccacccc gggtgaagag accgaccggc
     4021 aggccctgat acagaatgac cataccctgg acctgatgga acgctccttg tccgaactaa
     4081 accccgagca gcagcaatgt gtaactttgt tttaccttca gaaaaaaagt taccaggaag
     4141 tgagtgagga aaccggattc agtatgttac aggtgaagag ttatatacag aacgggaaga
     4201 ggaatctgag gttgctggtg gagaaaaaac taaaagaaaa aggaatttaa gtttttagct
     4261 gtgataaagc gaattatcca atatgccaat tagccaatgt gccaatgaaa gagtaattaa
     4321 agttcagaca gacaaaatga acagaactgc gtaataaaaa tgtcagaaaa tttaaaggac
     4381 atattatcca atgagcatcc ggaaattgat ccggagacca tgatgttata cctgcaggga
     4441 aaacttacgc ctgagcagaa gcatgaggtc gaaaaattgc tgatgcaggg agattttgaa
     4501 aacgaggcca tggaaggttt gcaggaattc aaggacaagg tacaaatcca gtatactata
     4561 gaagccctca accgtgacct gaagaaaaaa acggagaaaa agcaaaaacg ccgggataaa
     4621 ctcaaactgc cggatcaaac ctggataatt atttcagttg ttattgtatt gttacttgtg
     4681 atagtatctt atttcgtaat aaggaaatta ctgcagaatt agccaatttc taatacactc
     4741 ataagttctt atttactgac aatcaattaa ttaagaaata ttttccaggt tttccactgt
     4801 cttttcacgt accttctccc aatatgaact tactgttaac tttaggtaaa gtggaattag
     4861 ccaccctgaa gcaacctacc ttaccaacga ttaaaacaat ttgtcatgag atgggaaaaa
     4921 gtttctttta cgccagacaa tgaagtctat catttgtaca aggatgagca gaagatcctg
     4981 acactccatt ttcacccttt ttctcactca gcccgggttg aaaccggcaa tgaaaaacgg
     5041 gtattccaga tccgcaaaga aggctttctc cggaacaaga ccgtgttacg taccgaatat
     5101 ggtattaaac tcggcgaatt gggccatgag aacggccgta gttttatcaa tattgaaaac
     5161 gaacgctatt tttattccat catcaataat ccccttgccg aattacagat cttcaaagaa
     5221 caggcagaaa aacccatgat cacttgcgga ctcaaaaccg atgaaggaaa tccggaagta
     5281 cgttttaaaa gagacaagaa tcagttggac ggctcctatt ccagtctgct gatggcctta
     5341 tgctggtata tgttcttacc ggtagcaaaa gagaatacgc ccgagatggc aatttaacga
     5401 ctcttcttag aaagcttttt ttgactaagt attttttact gacttacaat tagcaggtaa
     5461 aatgtgccaa tgtgccaatt agccaatgtg ccaattgacc tactaagatt gttattcaaa
     5521 aatgtggttg acttgtgtta agttgaacct cttcagtatc attaacgacc atcgatcttt
     5581 tagccacttc cacaattggc acattggcat attgactaat tggctaatta taacctgagt
     5641 cctaaatcaa ctatcagccc tcgtataaag ttgtatttgt agtaacccta caacctctcc
     5701 caactttctt ttgcaaattg ctccagggaa ggatcccttg cacccataag caata
//
LOCUS       DAOPMW010000205         5745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16158_length_5745_cov_5.263093, whole genome shotgun sequence.
ACCESSION   DAOPMW010000205 DAOPMW010000000
VERSION     DAOPMW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16158_length_5745_cov_5.263093"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1264)
                     /locus_tag="PLU11_10065"
     CDS             complement(98..1264)
                     /locus_tag="PLU11_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59511.1"
                     /translation="MKRIFTFVFYILSLASCDTISKKAGVQAVNPDGSVMQEGKDYVI
                     LKRFRVMDQQGFSQPLEVSSFLLPANWTVNSDVSWNFTNKCLPDRLQASVQAVSPDKE
                     YELFVYPATQFDWSDDPIYLDAMQKGFVMHSCTIAQPQDAAGYINTIMAPYLNATVKS
                     AGIIEELQQQMNAGAMQMTQAARQGGNTAYNHKGSAAEGLLQFEEGKEGLAFCTLMQT
                     LVSVPGAQGGMSTNTQCYVSMRVVIKYKSGNEPMARKIMSTFFSSARINPQWAGGVQA
                     YFAAVTRNAQDEGWKQIQISHQAQQEIGDNIIRSWESRNAGSSSPSSDTDGFGQYLRG
                     VDNWTDDGGNKIELTSGYSNAWSRGDGSYMLSNNPAFDPNTMAGETQSWSRLKQ"
     gene            complement(1318..2490)
                     /locus_tag="PLU11_10070"
     CDS             complement(1318..2490)
                     /locus_tag="PLU11_10070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN59512.1"
                     /translation="MKSLQTFLLLFLSGILFSCSKSGGGSAPVPATQADIAAVDSKVA
                     TFMSTYNVPGASLAISKNGKLVYIKGYGKANTSTNELVTPAHRFRLASVSKTFTGVAI
                     LKLVQDGRLSLDAKVFGTGGVLAADYGTAPYNSNLLNITVRHLLQNVSGSWGASTGGD
                     VIDQNPSYTYKQLLDWIINTRPNPSAPGMVYDYSNVNFCIAGRIIEKISGKSYVNFIK
                     EDIMAPIGATQTDISGKTAGEKKANEVSYYGQGLDANFVYNIAFPRRDADGGLISTAS
                     DLLRLLNAIDGFSTRTDLLSPASITALTTPSAVFSSYACGIGIWSAENLWYNYGSLPG
                     TRTGFFRHNNGMCVALLLNSRADPSAGENPFVFAMQDLMLDLVKNSSYSWQNIDQF"
     gene            2621..4576
                     /locus_tag="PLU11_10075"
     CDS             2621..4576
                     /locus_tag="PLU11_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPN59513.1"
                     /translation="MMKRIGYLLLPALMTFCSAYGQFPGLDRAREAIYRATSDQERLS
                     RLQAAAAFKNSMPGDTIYHYAQWAKELALKTGNNKALAWAEYNLISGDISSGKTDSVI
                     PRIERNKLFIEIKKTDPDLFYKIQLLKANVLNRTNRRTEALELQLQLLSAAEKENNTL
                     ARLFLMNYTGATYFNTIGQQQAARKIWESGLEIIREANKPDYREIETYLLSNLALYHL
                     GNYYVKPSGALMDSCFLFLNHTIDLCRKNESMGVLASTLSYRGNLYGRSKLYPEAEAD
                     FKETVQIRSKIGDPLYMSEDFKNLSVFYFERNNLDKCLATVNEGLRLCEQFHIRESSL
                     ELLKLKAGVFHKKGDREQYTATLEKLLLVADSAYRFNTAEKIAKLQTEFEVQKKEALI
                     AKQQLDLLKRNFLLYAAGIATFLLALYFVFRFRKYKRKQQLLMEEKKQQHALQVKEAE
                     EKERKRIAAELHDNLGVQANAILYNSSQLTAGSGNPVQIANDLQETAKEMLLNLRETL
                     WAMKNSDVNAVQLWLRVINFMQQMGRHYTGIQFKVSGTAPKDFIIPANQALHMVLVIQ
                     ETVNNAVKHAGAGMISAHSTLYEKGWTIVIQDDGSGFDIGKAMAEEDRYGLKHIQERA
                     LAGAFICQLESEPGNGTKTTLQISDNR"
     gene            4628..5293
                     /locus_tag="PLU11_10080"
     CDS             4628..5293
                     /locus_tag="PLU11_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59514.1"
                     /translation="MIRVAIADDKVYNRKVIADKLSRSSLFTLSFQAANGNEFLQQMR
                     ELKQEELPHIVLMDLEMPEVDGVSAIGTASALYPSVKFVVLTIFDDDDKIFRAIRAGA
                     CGYLLKEESGEVITDMLQHLWENGAGPISPSIAYKILQMVQQPAPAVNDKNKAENIFQ
                     LSERELEILQLLCEGLEYKEIGTRIFISANTVKKHCISIYQKLHVNSKAQALRIAYTK
                     GLV"
     gene            complement(5312..>5745)
                     /locus_tag="PLU11_10085"
     CDS             complement(5312..>5745)
                     /locus_tag="PLU11_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HPN59515.1"
                     /translation="RIMGLPIVLPPLRERENDILLLAKHFADEFAKENNLGTIQFSQE
                     AKDKLMRHPFPGNVRELKAVIDLAVVMCEDNEIRDADITFAPGKREDFFLSEQKTLRQ
                     FTCDIVKQYLKKHNNDVVAAANALDIGKSTIYKMMQAGELD"
BASE COUNT         1599 a         1388 c         1322 g         1436 t
ORIGIN      
        1 ttatcgtacg ttcaggcaaa aatcagaagg aggtcaccac attatgacaa cctccatttt
       61 tcatagtaga aaggacggaa gaagccggat aaaatggcta ttgcttcaac cggctccagc
      121 tctgtgtttc accagccatc gtattcggat caaaggcggg attattactc agcatataac
      181 tgccatctcc ccggctccac gcattggaat agccactggt gagttcaatt ttattgccgc
      241 catcatcagt ccagttatcc acccctcgca ggtattgacc aaatccatcg gtgtccgatg
      301 atggactgct actacctgca ttcctggatt cccagctgcg gataatatta tcgccgatct
      361 cctgctgggc ctggtgactg atctgtatct gtttccagcc ctcatcctgc gcattgcggg
      421 taaccgctgc aaaataagcc tgcaccccac ccgcccattg cggattgatc cgggcactgc
      481 tgaaaaacgt actcatgatt ttccgggcca ttggctcatt acctgacttg tattttatca
      541 ccacccgcat actcacataa cactgcgtat ttgtagacat gccgccttgt gcgcccggta
      601 ccgaaaccag tgtttgcatc agcgtgcaaa aggccagccc ttctttgcct tcctcaaact
      661 gcaatagccc ttcggccgca ctgcctttat gattatacgc cgtattgcct ccctggcgag
      721 ccgcctgggt catctgcatc gctcccgcat tcatttgttg ttgcagttcc tcaatgatgc
      781 cggccgattt cactgttgca ttcagatagg gagccatgat tgtattgata taccccgcgg
      841 catcctgtgg ttgtgctatc gtacaggaat gcatcacaaa acctttctgc atggcatcca
      901 ggtaaatggg atcatcactc cagtcaaatt gtgtggccgg ataaacaaac aattcatatt
      961 ccttatccgg tgaaaccgcc tgcaccgatg cctgcaatct gtcaggcaaa catttatttg
     1021 taaagttcca actcacatca ctattcactg tccagtttgc gggtaataaa aaggaactga
     1081 cttcgagtgg ctgactgaaa ccctgttgat ccatcacccg gaaacgtttc agaatcacat
     1141 aatctttccc ttcctgcatc acagagccat caggatttac tgcctgcact cctgcttttt
     1201 tactgatggt atcacaggaa gcaaggctca ggatataaaa cacaaaggtg aatattcgtt
     1261 tcatgatttt taatggtttg aatgcctgat tcaaaaaaaa tcttccggca actatattta
     1321 aaactgatca atattctgcc aggaataact gctgtttttt acgaggtcca gcatcaggtc
     1381 ctgcatggca aaaacaaatg gattttctcc agccgacgga tccgcccttg aattgagcaa
     1441 cagggccaca cacatcccgt tgttgtgccg gaaaaaaccg gtgcgggtgc ctggcaaact
     1501 gccatagttg taccataaat tttccgcact ccagatacca ataccacagg cataagacga
     1561 aaacacagcc gaaggagtcg tgagtgctgt aatactggca ggggacaaca aatctgtacg
     1621 ggtgctgaat ccatcaatcg cattgagcag gcgtaataga tccgacgctg tggatataag
     1681 tccgccatcc gcgtcccttc tcgggaatgc gatattgtaa acaaaattgg cgtcaagtcc
     1741 ctggccataa taggaaactt cattggcctt cttctctcct gctgtttttc cactgatatc
     1801 ggtttgtgta gccccgatcg gtgccatgat atcttccttt ataaagttga cataggattt
     1861 accggaaatt ttctcaatga tcctgccggc aatacagaaa ttgacattgg aatagtcgta
     1921 caccattccg ggggcacttg gattcggccg ggtattgatg atccagtcca gcagttgttt
     1981 gtaggtatac gatggattct gatcaatcac atcgcccccg gtggatgcac cccatgaacc
     2041 agatacattc tgcaacaagt gacgaacagt gatattgagc aaattactgt tataaggtgc
     2101 tgttccataa tcggcggcca gcactccacc ggtgccgaac actttggcat ccagtgaaag
     2161 acgaccatcc tgtaccagtt tcaatatagc aacaccggta aaagtcttgg acacactggc
     2221 cagacgaaac cggtgagcag gtgtcaccag ttcgtttgta ctggtattgg ccttcccata
     2281 ccctttgata tatacgagtt ttccattttt gctgattgcc agagaggcgc cgggcacatt
     2341 gtaggtactc ataaacgtgg ccactttact atctaccgca gcaatgtctg cctgggtagc
     2401 gggtaccgga gctgatccac cgccggactt tgaacaggaa aataaaatac cgctcaaaaa
     2461 aagcaacagg aatgtttgca aggatttcat gtctgaataa ttagtgtttt attaaaacga
     2521 ataagggctt tgtaaaaatg gagggattcc ttacggtccc gtatactcca tttggatagt
     2581 tttttgaacg ggggtcgtcc ggtagtttta cataaattgt atgatgaaac ggattggata
     2641 cctgctttta cctgcgctga tgactttttg tagcgcctac ggacagtttc cgggtctcga
     2701 ccgtgcccgg gaagcgattt acagggccac cagtgaccag gaacgcctga gccggttgca
     2761 ggccgccgct gcctttaaaa attcaatgcc cggtgataca atctatcact atgcacaatg
     2821 ggcgaaggaa ctggcgctga aaaccgggaa taataaagcg ctcgcctggg ccgagtacaa
     2881 cctgatcagc ggggatattt ccagtggaaa aacagacagt gttattcccc ggatcgaacg
     2941 gaataaacta ttcattgaaa taaaaaaaac cgaccctgat cttttttata aaatccaatt
     3001 attaaaggcc aatgtactga accgtaccaa ccggcgtacc gaagcactgg aactgcaatt
     3061 gcaactattg agtgcggcgg aaaaagaaaa caataccctg gcacggctct ttttaatgaa
     3121 ctataccggc gccacctatt ttaataccat tggacaacag caagccgcgc ggaaaatatg
     3181 ggaaagcggg ctggagatca tccgggaggc aaacaaaccg gattaccggg aaattgagac
     3241 ttatctgctt tcgaatctgg cgctctatca cctggggaat tattatgtga agccctccgg
     3301 cgccctgatg gacagttgct ttttattcct gaatcatacc atcgacctct gccgcaagaa
     3361 tgaatcgatg ggtgtactgg cttccacttt atcctaccgg ggaaatttat acggccggag
     3421 taaattgtat cccgaggcag aagccgattt taaggaaaca gtgcagatcc ggagtaaaat
     3481 cggggaccca ttgtatatgt cagaggattt taaaaaccta tcggttttct atttcgaacg
     3541 taataacctg gacaaatgcc tggctaccgt caacgaggga ctccggctct gtgaacaatt
     3601 tcatatacgg gagtcatcac tggaactatt gaaattaaaa gccggcgtat tccataaaaa
     3661 aggggatcgg gaacaataca ccgctacact tgagaaactg ttactggtgg ctgactctgc
     3721 ttaccggttt aatacagcgg aaaaaatcgc caagcttcaa actgagtttg aagttcagaa
     3781 aaaagaagcc ctgatcgcca aacagcaact ggatctgctc aaacggaatt tcctgcttta
     3841 tgcggcggga atcgccactt tcctcctcgc cctgtatttt gtattccgct tcagaaaata
     3901 caaacggaaa caacagctgt tgatggagga aaagaaacag caacatgcgc tccaggtaaa
     3961 agaagcagag gaaaaagaac gaaaaagaat cgccgccgag ttgcatgaca acctgggcgt
     4021 gcaggccaat gccatcctgt ataacagcag tcagcttact gcaggatcgg gaaacccggt
     4081 acagattgcc aatgacctgc aggaaaccgc caaagaaatg ctgttgaacc tgcgggaaac
     4141 tttatgggcg atgaaaaact cggacgtgaa tgccgttcaa ctctggctgc gggtcatcaa
     4201 ttttatgcaa cagatggggc gtcattatac gggcatccag tttaaagtaa gcggcacggc
     4261 acccaaagac tttatcatcc cggccaacca ggcactgcat atggttctgg tgatccagga
     4321 aaccgtgaat aatgcggtaa aacatgcagg agccggcatg atcagtgcgc atagtacatt
     4381 atatgaaaag ggatggacca ttgtaattca ggatgacggc agcggatttg acatcggaaa
     4441 agcaatggcg gaagaagacc gctatggact caaacatatt caggaacggg cattggctgg
     4501 tgcattcatc tgtcagcttg aatcagaacc gggcaatggc acaaagacca ccctgcagat
     4561 cagcgataac cggtaaagca aagcccggca ttggccagaa aattgtttta cttccttata
     4621 tttaattatg atcagagtag ctattgcaga cgataaagta tataaccgga aggtgatcgc
     4681 tgataagctg tcccgaagca gcttgtttac gctcagcttc caggctgcga atgggaatga
     4741 atttttacag cagatgcggg aactgaaaca agaggaactt ccgcatattg tgctgatgga
     4801 cctggaaatg ccggaagtgg atggtgtgtc cgccatcgga acagcttccg cgctctatcc
     4861 ttctgtaaaa tttgtcgtcc tgaccatttt cgatgacgat gataaaatat tcagggcgat
     4921 ccgggcgggc gcctgtgggt atttattaaa ggaagaaagc ggggaagtaa tcaccgatat
     4981 gctgcaacat ctgtgggaaa acggcgccgg tccgatttca ccgagcattg cctataaaat
     5041 attgcagatg gtgcagcaac cggctcctgc cgtgaatgat aaaaacaaag cggagaatat
     5101 ttttcagtta agtgagcgtg aactggaaat cctgcagcta ttatgtgagg gactggaata
     5161 caaagaaatc ggcacccgga ttttcatcag tgccaatact gtaaagaaac attgcatcag
     5221 catttaccag aaactgcatg taaacagtaa ggcccaggca ctgcggattg cctataccaa
     5281 gggactggtg taaataacga agcgtaaagg attaatccag ttctccggcc tgcatcatct
     5341 tataaatagt gcttttcccg atatccagcg cattcgcggc agccaccaca tcattattat
     5401 gcttcttcag gtattgtttt acaatatcac aggtaaactg tcgaagcgtt ttctgctcac
     5461 taagaaaaaa gtcctcccgc ttacccggtg caaaagtgat atcggcatcc cgtatttcat
     5521 tgtcctcaca catcaccacg gccaggtcga ttaccgcttt aagctcacgt acattacccg
     5581 ggaatggatg acgcatcagt ttgtcctttg cttcctggct gaactggatc gtgcccaggt
     5641 tattttcttt ggcaaactca tccgcaaaat gcttggccaa taaaaggata tcattctccc
     5701 gttcacgcag tggcggcaac acgatcggca aacccatgat ccgga
//
LOCUS       DAOPMW010000206         5733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16214_length_5733_cov_5.973230, whole genome shotgun sequence.
ACCESSION   DAOPMW010000206 DAOPMW010000000
VERSION     DAOPMW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16214_length_5733_cov_5.973230"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLU11_10090"
     CDS             <1..750
                     /locus_tag="PLU11_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN59516.1"
                     /translation="GFDLLYKSGLLQLIFPPMVELAGAEYKDGMGHKDNFYHTLQVLD
                     NLSVHTPDLWLRWAAILHDIAKPATKRFEEGHGWTFHGHEAVGGRMVPKIFAKLKLPQ
                     NEKMRLVKKLVELHLRPISVTKENITDSAIRRLLFDAGDDFDALMMLCEADITSKNKQ
                     KVKRFLENFQLVRQRCKEVEEKDHVRNWQPPITGELIMETFGLSPSKPVGIIKDALKD
                     AMLDGLIPNTYEAAFAFMLEKAASMGILPVK"
     gene            799..1359
                     /locus_tag="PLU11_10095"
     CDS             799..1359
                     /locus_tag="PLU11_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59517.1"
                     /translation="MAILKFRVYFEEDESVYRDVAIRHTQTFKDLHDVILKAYEFDNK
                     HKATFFRSNDHWLRGREISLEVYDKEYKAPPLIMGDTTIGSEIRDPNQRFIYLYDFNK
                     NWGFLVELINVSKEENPKLSYPAVIRTEGIAPSQYGTKGLLGEKFADIEEKYDLTQVG
                     EGFGEKDESGEDVALDEGTTGEEEEI"
     gene            1360..2274
                     /gene="miaA"
                     /locus_tag="PLU11_10100"
     CDS             1360..2274
                     /gene="miaA"
                     /locus_tag="PLU11_10100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPN59518.1"
                     /translation="MNSTTPKTVILITGPTASGKTAVAIELARVYQTEIISADSRQCF
                     REMAIGVARPSSAELGAVPHHFIASHSIQEEVTAATFEKYALNKTATLFQEKDVVIVA
                     GGTGLYIRAFCEGMDQIPEIDPAIRQQVIREYKKNGLGWLQSSLQEKDPAFYSSGEIQ
                     NPQRSMRALEVVLSTGKSILTFQTGKKTKREFNVIKIGLDLPKEILHQQIHRRVDQMI
                     TTGLEQEVKSLLLFRQHNALQTVGYTEMFEYLDGKLSLEQAVEAIKTHTRQYAKRQLT
                     WFRRDKDIHWFSPHDTGAIKSFLQTSMC"
     gene            complement(2271..3962)
                     /locus_tag="PLU11_10105"
     CDS             complement(2271..3962)
                     /locus_tag="PLU11_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN59519.1"
                     /translation="MKYIVIGAVAGGASAAARLRRLNEKAEIVIFEKGEYISYANCGL
                     PYYIGDVISDRNKLFVQTATSFRNRFNIDVRISTEVTGIDAMNKTVSARNLLTGEEYT
                     ESYDKLVLSPGAEPIRPPLPGIGLPGIFTLRNVTDTDFIKQYVDQRKSGRAVVIGAGF
                     IGLEMAENLHDLGMRVSVVEMGNQILAPVDFPVAAMAQQHIRSKGVDLRLSSAVTGFE
                     KENDTLKVLLKDGTVLDADVVILSIGVRPDTRLAVAAGLDLGAAKGIWVNEYLQTSHP
                     DIYAVGDAIEFANPVTGQSMNTYLAGPANKQGRIAANNIVLGNKHLYNGSINTAIVKV
                     FDMTVATAGTASKHLKMAGINHIVSTNHSGSHAGYYPGAKQMTIQIAFSPENGRLYSA
                     QIAGYDGVDKRIDILASVIKNKGTIYDLTEFEHAYAPPYSSAKDPVNMAGFIAENILT
                     DRLNIFYWNDLGDGDDNSYLLDVRTVREFEAGHIEGAVNIPVDEIRDRLNEIPAGKRI
                     DIYCEAGLRGYLASRILRQNGFNEVFNLSGGYVLWNACTRETALTKPVQQVAATV"
     gene            complement(4199..4918)
                     /locus_tag="PLU11_10110"
     CDS             complement(4199..4918)
                     /locus_tag="PLU11_10110"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPN59520.1"
                     /translation="MTKLSVNINKLATLRNSRGGNNPDLLKAAVDVQLFGADGVTVHP
                     RPDERHIRYEDVRQIRKIISTEFNIEGNCTEQKFVDLVLETKPDQVTLVPDALGQLTS
                     DHGWDTINNKDYLRDIISVFKKAGIRVSIFVDPVVEMVIGAAETGTDRIELYTEGYAR
                     ESGVGRRESGVAPYITAAIKAKEIGLGLNAGHDLDLNNLQFFKQHIPWLDEVSIGHAL
                     ICDAIYLGLENTIQLYKRQLQ"
     gene            complement(4940..>5733)
                     /locus_tag="PLU11_10115"
     CDS             complement(4940..>5733)
                     /locus_tag="PLU11_10115"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HPN59521.1"
                     /translation="TMVQAYNFLASWQLFPEKGVYEYGERPKSGIYKIEAVADKKELQ
                     IFHNWVTLENQAFSSGYRINADGELNTFENPELAEKVQALIPDGITFEIHFYKKGEIA
                     LHVVHEIMPNGYLRVTQQSRRPDGTSYTNMEYYHKQMSVLPYSASVSGAVIKPTEEGV
                     IKHKALTAMEEQTNMQLDQIRKQIELLALQAHEIQQRKELSMMIYNARLSFKPNIGQT
                     YYLYEKNDDSFMLSLVSPKEWGGTGPFKKFVATVQLLADHTWKEL"
BASE COUNT         1491 a         1340 c         1343 g         1559 t
ORIGIN      
        1 ggatttgacc tgctttataa aagcgggttg ttacaactca tctttccgcc tatggtagag
       61 ctggcgggtg cggagtacaa ggatgggatg gggcataagg ataattttta ccataccctc
      121 caggtgctgg acaacctctc tgtgcatacg cccgatctgt ggttacgctg ggcggccatc
      181 ctgcatgata ttgccaagcc ggccaccaaa cgttttgaag aagggcatgg ctggactttt
      241 catgggcatg aagcggtggg tgggcgaatg gtgccgaaaa tttttgccaa actcaaactg
      301 ccgcaaaatg agaaaatgcg gctggttaaa aaactggtcg aattgcacct gcgtccgatc
      361 agtgtgacca aagaaaatat cacggattcc gccatccgtc ggttactttt tgatgccggc
      421 gatgattttg atgccctgat gatgttgtgt gaagccgata tcacgtctaa gaataaacaa
      481 aaagtcaaac gtttcctgga gaacttccag ctggtacggc aacgttgtaa agaagtcgag
      541 gaaaaagacc atgtccgcaa ctggcagcca ccgattaccg gagaactcat catggaaaca
      601 ttcgggttgt ctccttccaa accagtgggg ataatcaagg atgcgttgaa agacgctatg
      661 ctggatggcc tcattcccaa tacctatgag gcagcctttg catttatgct tgaaaaggca
      721 gcttcgatgg gaattttacc cgtaaaataa ttcaataaca aaattcgggc tgaaggggat
      781 taatcttaaa tttgtgctat ggccatttta aaattcagag tctattttga ggaggacgaa
      841 agcgtgtacc gtgatgtggc gatccgtcat acccagactt ttaaggacct gcatgatgtc
      901 attctgaaag catacgagtt tgacaataaa cataaagcga cttttttccg cagcaatgac
      961 cattggctgc gtggacgtga gatttccctt gaggtttatg ataaggaata caaagcacct
     1021 ccactgatca tgggtgatac cacgatcggt tccgaaatcc gcgatcccaa tcagcggttt
     1081 atctatctct acgattttaa taagaactgg ggcttcctgg tggaactcat caatgtatcc
     1141 aaagaagaaa accccaaact cagttatccg gctgtgattc gtacagaagg cattgctccc
     1201 agccagtacg gcaccaaagg tttactcggc gaaaaatttg cggatatcga ggaaaagtat
     1261 gacctgaccc aggtcggtga gggcttcggg gaaaaggatg agtcaggcga agatgtagcc
     1321 ctggatgaag gaacaaccgg ggaagaagaa gagatataat tgaatagcac cactccaaaa
     1381 accgttatac tgattaccgg gcctacggct tcgggaaaaa cagctgtggc tattgaactg
     1441 gcccgggttt atcagactga aatcatatcg gccgacagcc gccaatgctt ccgggaaatg
     1501 gcgattggtg tggcccgtcc gtcatctgcc gaactgggtg ccgttcccca tcattttatt
     1561 gcttctcata gtattcaaga ggaagtaaca gcagctactt ttgagaaata tgcccttaat
     1621 aaaacagcaa cactcttcca ggagaaagac gtagtaatcg tggcaggtgg gaccggttta
     1681 tatatccggg ctttttgtga gggaatggac cagatccctg aaattgatcc ggctatccgg
     1741 cagcaggtga tccgtgagta taaaaaaaat gggcttggct ggttgcaatc ttccttgcaa
     1801 gaaaaagacc cggcttttta ttccagcggc gaaattcaaa atccgcagcg aagtatgcgg
     1861 gcgctggaag tcgttctttc caccggtaaa agtattctga cctttcagac aggaaaaaaa
     1921 acaaaaaggg aattcaatgt cataaaaatc ggacttgatt tacctaagga aatattacat
     1981 cagcagattc accggcgcgt tgatcagatg atcactaccg gtcttgagca ggaggtgaaa
     2041 tccttattgc ttttcagaca gcacaatgcc ttgcagactg ttggctacac tgaaatgttt
     2101 gagtacctgg atggaaaact cagcctcgag caggcggttg aagccatcaa aacccatacc
     2161 cggcaatatg ccaaacgaca gctgacctgg ttcagaagag acaaggatat ccactggttt
     2221 tcaccgcatg ataccggagc cattaaatcc tttttacaga cttcaatgtg ttaaacggtg
     2281 gctgctacct gttgtaccgg ttttgtcaaa gccgtttccc gggtacaggc attccacaat
     2341 acatatccac cggagaggtt aaagacttca ttaaatccgt tctgccggag aattcggctg
     2401 gccaggtaac ctcttaatcc tgcttcacaa tagatatcga tgcgtttgcc ggcgggaatt
     2461 tcattcagtc tgtcacggat ctcatccacc ggaatattca ctgctccttc aatatggccc
     2521 gcttcaaatt ccctcacggt tcttacatca agcaggtaac tgttgtcgtc gccatccccc
     2581 aggtcattcc agtaaaatat attcaaccgg tccgtcaaaa tattttctgc tataaaaccg
     2641 gccatattca ccggatcctt ggcagaggaa tagggagggg cataggcatg ttcaaattca
     2701 gtgaggtcat agatcgtgcc tttgtttttt attaccgaag ccaggatgtc tatccgtttg
     2761 tccacaccat catatcccgc aatctgggca ctgtacagcc ggccattttc cggggagaat
     2821 gcgatctgga tcgtcatttg ttttgccccg ggataataac ccgcatggga gccgctgtga
     2881 ttggtggata cgatatgatt tattccggcc attttcaaat gcttgctggc ggtaccggcg
     2941 gtagccacgg tcatgtcaaa aacttttaca atcgctgtat tgattgatcc attgtacagg
     3001 tgtttatttc cgagtacaat attattggcg gcaatacgtc cctgtttatt agcaggacct
     3061 gccaggtagg tattcatgga ttgtccggtt accggattgg caaactcaat cgcatctccc
     3121 acggcataaa tatccggatg tgatgtctgc aggtattcat tcacccagat gcctttggcc
     3181 gcgcccaggt cgagtccggc tgctacggca aggcgggtgt caggtctgac cccaatagaa
     3241 agaataacca catccgcatc cagtactgta ccatctttga gtaatacttt caatgtatca
     3301 ttttcttttt caaaaccggt caccgcactg ctgagccgca ggtccacccc tttggagcgg
     3361 atatgttgct gtgccatggc ggctaccgga aaatcaaccg gtgccaggat ctgattgccc
     3421 atttcaacta cggagacgcg catgccgagg tcatgcaggt tttctgccat ctccagtccg
     3481 ataaaaccgg ccccgatcac caccgcacga ccgcttttcc gttgatccac atattgtttg
     3541 atgaagtcgg tatccgtaac attccgcagg gtgaatatgc ctggtaatcc aatacccggg
     3601 aggggcggac ggataggttc tgctcccgga gagagaacca gtttatcata tgattcggta
     3661 tattcttctc cggtcagcag gttacgggca ctgacagttt tattcatggc gtctatacca
     3721 gtcacttctg tggaaatacg tacatcaata ttgaaccggt tacggaaaga ggtggcggtt
     3781 tgtacaaaca atttattgcg gtcgctgatt acatcaccaa tataataagg cagtccgcag
     3841 ttggcatagg agatatattc ccctttttca aatattacga tctcggcttt ttcatttaat
     3901 cttctcagac gggcggcggc actggctccc ccggctaccg cgcctatgac aatgtacttc
     3961 atgtagtgct tttatttgta aaggttgaaa ggttgaaaag tttataagtt gaaaaggtat
     4021 gttctaagaa aacttccctg ttaacctttc aacttttcaa cttgtcaact tcatttgact
     4081 cagcaaaaga ataccataaa tagacaacgg aatgtaacct tggttacgca agaggggaca
     4141 ggcaggcagg gaggaatatg attttcgtca tataacaggt tctcttcgta tatatgcctt
     4201 attgtaactg tcttttataa agctgaatgg tattttccaa acccaggtag atcgcgtcac
     4261 aaatcaacgc gtgcccgata cttacttcat cgagccaggg tatatgctgc ttaaagaatt
     4321 gcagattatt caaatccagg tcatgtccgg cgttcagtcc caatccgatt tctttggctt
     4381 ttattgctgc tgttatataa ggggcaacac ccgactcccg tctcccgact cctgactctc
     4441 tggcgtaccc ttccgtatac aactcaatcc tgtctgtccc ggtttccgcc gctccaatca
     4501 ccatttccac caccggatct acaaaaatgc tgacgcgtat acccgctttt ttgaaaacgg
     4561 aaatgatatc ccggagatag tctttgttgt tgatcgtatc ccagccatga tcactggtca
     4621 gttgtcccag ggcatcgggc accagggtta cctggtcggg tttggtttcc agtaccaggt
     4681 ccacaaattt ctgttcggta caattgcctt ctatattgaa ttcggtgctg atgatttttc
     4741 tgatctgacg gacatcttca taccggatat gccgttcatc cgggcggggg tgaacggtta
     4801 ctccatcagc gccaaataac tggacatcca cagctgcttt taacagatcc ggattattgc
     4861 cgcctcttga gttgcgaagg gtggccagct tattgatgtt taccgaaagt ttcgtcatgc
     4921 cgcaaaagta caaaaagcat cagagttctt tccaggtatg atccgccagt aattgaaccg
     4981 tggccacaaa ttttttaaaa gggccggttc ctccccattc tttcggagat accagggaga
     5041 gcatgaacga gtcatcgttt ttttcgtaga gataataggt ctgcccgata ttgggtttga
     5101 atgaaagccg ggcattatag atcatcatcg ataattcttt ccgttgctgg atttcgtgtg
     5161 cctgaagggc cagcaattca atctgcttcc ggatctggtc cagttgcata ttggtctgtt
     5221 cttccattgc tgtcagggcc ttgtgtttga tcaccccttc ttcagtcggt ttgatcacgg
     5281 cgcctgaaac ggaggcggaa taagggagca cactcatctg tttgtgatag tactccatat
     5341 tggtataaga agtgccatcc ggccggcggc tttgctgggt tacccgtaaa tatccattgg
     5401 gcatgatttc atgtaccacg tgcaaagcaa tttccccttt tttataaaaa tggatttcaa
     5461 acgtgatacc atctggaatc agggcctgca ctttttcggc cagctccgga ttttcaaaag
     5521 tattgagttc gccatccgca ttgatccggt aaccggaaga aaaggcctga ttttccaggg
     5581 taacccagtt atgaaaaatc tgcaattctt ttttgtctgc tacggcttca attttgtaaa
     5641 taccgctttt gggacgttca ccatactcat aaaccccctt ttcagggaat aactgccagg
     5701 aagcgagaaa attataggcc tgtaccatag tgc
//
LOCUS       DAOPMW010000207         5727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16236_length_5727_cov_6.666079, whole genome shotgun sequence.
ACCESSION   DAOPMW010000207 DAOPMW010000000
VERSION     DAOPMW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_5727_cov_6.666079"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..841)
                     /gene="argB"
                     /locus_tag="PLU11_10120"
     CDS             complement(53..841)
                     /gene="argB"
                     /locus_tag="PLU11_10120"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPN59522.1"
                     /translation="MDKLYIIKIGGNIIDDEAKLATFLRDFAAIEEKKILVHGGGKLA
                     TRLAEKMGIEQQLVDGRRITDAETLKIVTMVYAGFINKNIVATLQSNHCNAIGLCGAD
                     GDVILAHKRKHPVLDYGFVGDVDSINTDLISTLLHKNISIVFAPITHDQQGQLLNTNA
                     DTIAQEIAKGMSGLYDTELIYSFEKSGVLLDANDDSTVIPEIRPSYYLELKAKNKIFA
                     GMIPKLDNAFTALNSGVSKVIIGKAEQLSDLLQGKAGTSILHEQ"
     gene            complement(834..1799)
                     /locus_tag="PLU11_10125"
     CDS             complement(834..1799)
                     /locus_tag="PLU11_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HPN59523.1"
                     /translation="MKNFVSVHDVNNIDALVQRALAHKANPLAHKTLGANKRIGCLFL
                     NPSMRTRLSTQIAAQNLGMEAIVFNVGSEGWALEFEEEAIMSGTSVEHVKDAAPVFGK
                     YFDILAIRTFPSLKNREDDYSELYIKQFIKYAGIPVLSMESATLHPLQSLTDIITITE
                     SMQEQPLNHKPKIVLSWAPHVKPLPQCVANSFSQWVNAWGQADFVITHPEDYELSEEF
                     TKGATITHNQEEALKDADFVYVKNWSTYTDYGKIYESDPKWMLTNKKLEATNNAKVMH
                     CLPVRRNVELSDEILDGPNSIVTREAGNRVWAAQAVLEQLLTTNG"
     gene            complement(1801..2952)
                     /locus_tag="PLU11_10130"
     CDS             complement(1801..2952)
                     /locus_tag="PLU11_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN59524.1"
                     /translation="MNLFDVYPINEVTIVKAKGSYVWDEKGDQYLDLYGGHAVISIGH
                     THPHWVKRIEEQLEKIAFYSNSIKIPLQVQLAEKLGKVSGKEDYQLFLCNSGAEANEN
                     ALKLASFHTGRKKIVAFSKSFHGRTSLAVAVTDNKNYSAPVNETDNVIFLPFNDGEAL
                     EACFAKEGKNIAAVIIEGIQGVGGINVADDTFLRSIRSLCDTHGAMYIADSVQCGYGR
                     SGKFFSHDFAGVSADIYTMAKGMGNGFPVAGIIIAPHIKPKHFQLGTTFGGNHLACAA
                     ALAVLEIIEEEKLMGNAVMIGKYLRDELNAIPELKNVRGRGLMIGFDVPDELKDLKKN
                     LLWKQKIFTGEAKPNVIRLLPSLGLKKKDAEQFIESIKEEVNLLTTPAS"
     gene            complement(3007..3372)
                     /locus_tag="PLU11_10135"
     CDS             complement(3007..3372)
                     /locus_tag="PLU11_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN59525.1"
                     /translation="MQNFKDLKVWEKAHHFTLRVYEVSRAFPKEEVYGLTSQLKRAAS
                     SIPSNIAEGCGKNSQNDFANFLNIALGSANESEYFLILAKDLTYLKLPAFEELSVTIN
                     EIKAMLISLLTKVRSNTKN"
     gene            complement(3408..4397)
                     /gene="argC"
                     /locus_tag="PLU11_10140"
     CDS             complement(3408..4397)
                     /gene="argC"
                     /locus_tag="PLU11_10140"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPN59526.1"
                     /translation="MSINIGIIGGAGYTGGELIRLLIHHPEAEIRMIHSRSNAGKAVS
                     AIHQDLIGETSLTFTDDSSPLEKGSIDVLFLCLGHGESKKFLAENTIAASIKIIDLAN
                     DFRLAAHANAGNRQFVYGLPELNRDIIKTAKNIANPGCFATAIQLGLLPLAKAGLLAD
                     IYTTGITGSTGAGQSLAATSHFSWRANNIQAYKTLTHQHLGEIGESLLQLQPVNDVDV
                     HFVPWRGDFTRGIFISSTLHCDESLEEMYTLFEEYYKGHPFTTVSREPIFLKQVVNTN
                     KAVIQLEKAGTKLVVHSSIDNLLKGASGQAVQNMNLVCGLEETTGLKLKANYF"
     gene            complement(4586..>5727)
                     /locus_tag="PLU11_10145"
     CDS             complement(4586..>5727)
                     /locus_tag="PLU11_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPN59527.1"
                     /translation="YCVKYLGEDKGYEVHSIIVNTGGFTEEELKKIEAHAYALGVKTH
                     TTIDAVKGYYDRIIKYLVYGNVLKNNTYPLSVSAERLSQALHIAEHAKKLNADAVAHG
                     STGAGNDQVRFDMIFHIMIPGVEIITPIRDLKLSREEEISYLKTKGVDMNFAKAMYSI
                     NKGLWGTSVGGKETLSSKGMLPEEAWPTQVTKTGTEEVKLGFVKGELTTVNDQSFGHP
                     TEAIQYLQTIAGAYGIGRDIHVGDTIIGIKGRVGFEAAAPMLILKGHHALEKHVLTKW
                     QLQWKDQLASFYGNWLHEGQILDPVMRDIEAFLSNSQQNVTGEVFIQLMPYRFQVIGI
                     ESEFDLMNSKFGKYGEMNTGFTGDDVRGFSKIFGNQTSIWKAVNE"
BASE COUNT         1344 a         1353 c         1342 g         1688 t
ORIGIN      
        1 ggcgatcagc tctttcagca gttcaatggc gttggtttgt aatatggtta gttcactgtt
       61 catgtaaaat acttgtacct gcctttcctt gtaacaggtc ggacagttgt tccgctttgc
      121 cgatgatcac cttgctcacc ccgctgttca gcgcggtaaa agcattgtcg agtttgggga
      181 tcattccggc aaaaatcttg ttttttgctt tcagttccag gtaataagaa ggcctgatct
      241 ccgggatcac cgtgctgtcg tcattggcat ccagcaacac gccacttttt tcaaaagaat
      301 agatcagctc cgtatcatac aggccgctca ttcctttggc aatctcctgc gcaatggtat
      361 ccgcgttggt attcagtaat tgtccttgct gatcatgagt gatcggggca aagacaatgg
      421 aaatattctt gtggagcagg gtactgatca ggtcggtatt gatgctatcc acatccccga
      481 caaatccata atccaatacc gggtgttttc ttttatgggc gagtatcaca tctccatcgg
      541 cgccgcaaag tccgatcgcg ttgcaatgat tggactgaag cgtagctacg atattcttat
      601 tgataaaacc ggcatagacc atggtcacga tcttcagggt ttcggcatcg gtgattctcc
      661 gtccgtccac taattgctgc tcaataccca ttttttcggc cagtcgggtg gccagtttac
      721 cacctccgtg aaccaatatc tttttttctt caatggcagc aaagtcgcgc aggaaggttg
      781 cgagtttggc ttcatcatca atgatgttgc ctccgatctt tatgatatag agtttatcca
      841 ttggtcgtta aaagttgttc caataccgcc tgcgcagccc atacccggtt accggcttca
      901 cgggtcacga tgctgttggg tccgtcaagg atctcatcac tgagttccac attacggcgc
      961 accggcaggc aatgcatgac tttggcgttg ttggtcgcct cgagtttttt attggtcagc
     1021 atccatttcg ggtcgctttc atagatcttt ccgtaatcag tatatgtact ccagttcttt
     1081 acatagacaa agtcggcatc tttcaatgct tcttcctggt tatgggtaat cgtggctcct
     1141 ttggtaaatt cctcactcag ttcataatct tccgggtgag tgatcacaaa atctgcctga
     1201 ccccaggcat tcacccattg ggaaaaactg ttggccacac attgaggaag tggcttcaca
     1261 tgcggagccc agctcaatac gattttcggt ttatggttca gcggttgttc ctgcatggac
     1321 tccgttatgg taatgatatc agtcagcgac tgcagcggat gcagggtggc actttccata
     1381 ctcagcaccg ggatacccgc gtatttgata aattgtttga tgtatagttc gctgtaatca
     1441 tcttcccggt ttttcaatga ggggaaagta cggatggcga ggatatcgaa atattttccg
     1501 aataccggcg cggcatcctt cacgtgttcc accgatgtac cactcatgat ggcttcttcc
     1561 tcaaactcga gggcccagcc ttcactgcct acattgaaga caatagcttc catgcccagg
     1621 ttctgcgcgg cgatctgtgt actcagacgg gtacgcatac tgggattgag gaataaacac
     1681 ccgatccgct tattggcacc aagtgtttta tgggccagcg ggttggcttt atgggccagg
     1741 gcacgttgta ccagggcgtc aatattgttt acatcatgaa cagaaacgaa atttttcata
     1801 tcagctggca ggggttgtaa ggaggttgac ctcctctttt atggattcga taaattgttc
     1861 ggcgtctttt ttctttaaac caagggaagg taagaggcgg atcacattgg gtttggcttc
     1921 cccggtaaag atcttttgtt tccagagcag gtttttcttc aggtccttca gttcgtccgg
     1981 cacatcaaac ccgatcatca gtccgcggcc cctgacattt ttcagttcag gaatggcatt
     2041 cagttcgtcc cgcaggtatt tgccaatcat gacggcattg cccatcagtt tttcttcttc
     2101 gatgatttcc agaaccgcca atgccgcagc acaagccagg tgattaccac caaacgtagt
     2161 gcccagctgg aaatgtttgg gtttgatatg gggagcaatg atgatgcctg ctaccggaaa
     2221 tccatttccc attcctttgg ccatggtata gatatcggca cttacgccgg caaaatcatg
     2281 actgaagaat ttgccgctcc gtccataacc acactgtaca ctgtcggcaa tgtacatggc
     2341 gccatgtgta tcacaaagtg aacggatact gcgcaggaaa gtgtcatcgg caacatttat
     2401 gccaccaact ccctgtatgc cttcaataat cacggcagcg atgttttttc cttcttttgc
     2461 gaaacaggct tcaagcgcct ctccatcatt gaaaggcagg aagatcacat tatcggtttc
     2521 attgaccggg gccgaatagt ttttattatc cgtcaccgcc acagcgagtg aagtccgtcc
     2581 gtggaaggac ttactgaagg cgacgatctt tttacgaccc gtatggaaag aggcaagttt
     2641 cagcgcattt tcattggctt cggcgccgct gttgcagagg aataactggt agtcttcctt
     2701 gccggatact ttacccagtt tttcagccaa ttgtacctgc aggggtattt taatggagtt
     2761 ggaatagaag gcaattttct ccagctgctc ttcaatccgc tttacccaat gcgggtgggt
     2821 atgaccgata ctgatcaccg catggccgcc atacaggtca aggtactgat cgcctttttc
     2881 gtcccataca taggagcctt tggcttttac gatggtgact tcgttgatag gatatacatc
     2941 aaagaggttc ataagagtta taagttataa gttgtaagtt ataagtaatg tactaagggg
     3001 tactggttaa ttttttgtat ttgaacgaac tttggttaaa agtgaaatga gcattgcttt
     3061 tatttcattg attgttactg ataattcctc aaaggcagga agtttcagat atgtaaggtc
     3121 tttggcaaga atcaggaaat attctgattc attggcagag ccaagtgcta tgttcaggaa
     3181 gttggcaaag tcattctgag agtttttgcc acagccttca gcaatattgg atggaattga
     3241 agatgcagct ctctttaact ggcttgtcag gccataaact tcctctttag ggaaagcccg
     3301 tgacacttca tacactctca atgtgaagtg atgggctttt tcccaaactt taaggtcctt
     3361 gaaattctgc atacttataa cttactactt acaacttacc actgctctta gaaatagttt
     3421 gccttcaatt tcaaaccagt tgtttcttcc agaccgcaaa ccaggttcat attctgcacc
     3481 gcttgtccgc tggcgccttt taataaatta tcgatggagg aatgcactac cagtttagtg
     3541 ccggctttct ccaattggat gaccgcttta ttggtgttta caacctgctt gaggaaaatg
     3601 ggttcgcggc ttacggtggt aaaggggtgg cctttgtagt actcctcaaa gagtgtatac
     3661 atttcttcca ggctttcgtc acaatgcagg gtggaactga taaaaatgcc gcgggtgaaa
     3721 tcgccgcgcc aggggacaaa atgaacgtcc acatcattta ccggttgtaa ctgcagcagg
     3781 gattctccga tttccccgag gtgctggtgg gtgagcgttt tgtaggcctg gatattattg
     3841 gccctccagc tgaaatggga ggtggccgcc agggactgac ctgcacccgt tgaacccgtg
     3901 ataccggtgg tatagatatc tgccagtaaa cctgctttgg ccaatggcaa taaacccagc
     3961 tggatagcgg tcgcaaaaca accgggattg gcaatatttt ttgctgtctt tatgatatcg
     4021 cgattgagtt caggtaaacc atatacaaac tgacggttac cggcatttgc atgagccgct
     4081 aacctgaaat cattggccag gtcgatgatc ttaatggagg cggcgattgt attttctgcc
     4141 agaaactttt tggattcacc atggccaaga caaagaaaca atacatcaat gctccctttt
     4201 tcgagcggac tgctgtcatc agtaaaggtc agggaagttt ccccgatcag gtcctggtgg
     4261 atagcactca ccgctttgcc ggcattgctg cggctgtgaa tcatgcgaat ctccgcctcc
     4321 ggatgatgga tcagtaaacg gatcaattca cccccggtgt accctgcgcc tcctatgatg
     4381 ccgatgttga tggacatttg ttgctttatt tacgtattct atcttaatta cgaagattag
     4441 tagttaagat gtaaaaagtt gtcagttgtc agttgccggt tgtcagtaac agcccctcct
     4501 gagtttgttt atccttaaat aacaagaaca atcacttttg ctgacgatct ttcgtacttc
     4561 ggacttctaa cctcgtactt tctcctcact cgttcactgc cttccaaata ctcgtctgat
     4621 tcccgaaaat cttgctgaat ccgcggacat catcgccggt aaatccggta ttcatttcgc
     4681 cgtatttgcc aaacttactg ttcatcagat caaactcaga ttctataccg attacctgaa
     4741 aacgataggg catcagctgt ataaatactt cacctgttac attctgctgg ctgttgctga
     4801 ggaaggcttc gatgtccctc ataacggggt cgaggatctg tccttcatgc agccagttgc
     4861 catagaagga ggccagctgg tctttccatt gtaattgcca tttggtgagc acatgttttt
     4921 ccagggcatg gtgtcctttc aggatcagca tgggggcggc ggcttcaaaa cccacacggc
     4981 ccttgatacc gatgatggta tcgcccacgt ggatatcacg tccaatgcca taagcaccgg
     5041 cgatggtttg taaatattga atggcttcgg tgggatgacc gaaagactga tcattgaccg
     5101 tagtgagttc accttttaca aagccgagtt tcacttcttc agtgcccgtc tttgtaacct
     5161 gggtcggcca ggcttcttcg ggcagcatgc ctttggaaga aagggtttcc ttaccgccga
     5221 cactggtacc ccagagtcct ttgttgatgg agtacatggc tttggcaaaa ttcatgtcca
     5281 cgcctttggt cttcaggtag ctgatctctt cttcgcggct cagtttcagg tcgcggatcg
     5341 gcgtgatgat ttccacaccg ggaatcatga tatgaaagat catatcaaaa cgcacctggt
     5401 cgttgccggc gccggtgctg ccatgggcta ctgcatcggc attcagtttc ttcgcatgtt
     5461 cagcgatatg cagggcctgg ctcaggcgtt cggcggatac ggaaaggggg tacgtgttat
     5521 tcttcaacac gttgccatag accaggtatt ttatgatccg gtcataatag ccttttaccg
     5581 catcgatcgt ggtatgggtt tttacaccca acgcatatgc atgtgcttca atttttttta
     5641 attcttcttc ggtaaagcca ccagtattca cgatgatact gtgtacttca tagcccttgt
     5701 cttcacccag gtatttaacg cagtaag
//
LOCUS       DAOPMW010000208         5693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16364_length_5693_cov_7.358815, whole genome shotgun sequence.
ACCESSION   DAOPMW010000208 DAOPMW010000000
VERSION     DAOPMW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16364_length_5693_cov_7.358815"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLU11_10150"
     CDS             <1..1221
                     /locus_tag="PLU11_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59528.1"
                     /translation="DLKGYIHNPAWYFASDNDSVKMATDLLMMTHGWRRFRWQELAQK
                     VKQPLPFVDPSYITITGRVTLDGTNRAFGEKNLLALITAEGMKRNAQFISTDKQGNFK
                     LDSLLFFGSARIFILDIKGRKSQYIDVKMTSDSLGRPYPIAIPGSSDFATVNVKEKQK
                     QLAEDFDAIQKANGIMLEGITLKVRKKSPTEELDDKYTRGMFSGMAQKTLDLVNTDQF
                     ISENNIFDYLMSRVPGLDVTNEGAEYKVYYRQGPSLSSLGPIPMTLYLNEIETDASVI
                     ATLPATEIAMVKVYSQFVGAVGGGAGGAMAIYTRKGSDIVNSSRGDIIRYSGFTLVKD
                     FFAPNYKTDPALLKKPDTRITIDWRPNIFVNNIDPVIPISFYNSDRTKRFRVVVEGMT
                     TSGKLISLEEIISK"
     gene            1259..3718
                     /locus_tag="PLU11_10155"
     CDS             1259..3718
                     /locus_tag="PLU11_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59529.1"
                     /translation="MRFRMIVLGFLICGFTGSFLHAQDNLAKNNFMSEKVHLHIDRYV
                     FGAGETVWFKAYILSGDTSGLTSSVLHVELLFGDSLLSYQRLPVVLSVSNGQFFLADS
                     LVTGWYTVRAYTEAMLNQQPNSFFQSSIFIHGRKNGQKSQTDVFRDSMLVSFFPEGGN
                     LVKGLESTVAFKATDEWGFPVDFSGVVRAENGDSIAVANTFKFGMGEFKLRPSIGQRY
                     YVSTSVKGSIIKYYLPEVVDEGVALSMIYHPEGIYFELNQAGSNRMKKASVVVGHMFQ
                     KEVFRRKITDKENTFQGVLNTKQLTSGILAVTVLNDDGLPLAERLIFINNKEYRQAVK
                     IIADTVDFRSKARNYLKIALTDTVLGSLSLSITNADYGYTSVRPASIISDLLLTSELK
                     GDIVNPDWYFQEGNDSVSTGLDLLMLTQGWRNYKWAIHDKNSDLSKLYKDPAYITIRG
                     RALLRGTKSPLSDSRLMVVLSAKGITNNILLTETDKQGYFTIDSLLFFGKARFYFAEQ
                     REKKKARYIDIILSEGISSDKLLPEKRTRQLLVDRQHQHYQKIPDSIINYLEEITRAE
                     GKMMQGVTVTAKMKTPVEKLEDEYTVGAFSTNAFVERVIDLVNTNEPVVQPNIFEYLK
                     SRIPGLMIADPDLSKSPPDPSQPGFDPRNDPTKLRLFYRQMPSVSSLGNPPMVVYLNE
                     IETDPDVIATIPAAEIALIKIYSSFVSAPGAGPGGVMAIYTKKSKDMSDPLGSTISYE
                     GFSVIKEFFAPNYKADPSIIFKPDTRITIDWRPNIFVNNIDPVIPISFYNSDRTKRFR
                     VVVEGMTTSGKLISIEQIISK"
     gene            complement(3866..4489)
                     /locus_tag="PLU11_10160"
     CDS             complement(3866..4489)
                     /locus_tag="PLU11_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59530.1"
                     /translation="MLRRTTGRLLLLLLVITGSAGTIVNNSISKSDRKYAITLMKESQ
                     KDLVKATKGLSEAQLDFKAAPDRWSVKECVFHIAASEKMLWGMFENAMKAAPNPEKRT
                     EIKVKDEELVMMVKDRSKKNQAPEPIQPKNTGYKNLEEALADFKDTRTAHIRYMRTST
                     EDMRNRVVQLGTNWMDCYQLYLLIGAHSQRHTMQLNEVKAAAGFPKE"
     gene            4631..5656
                     /locus_tag="PLU11_10165"
     CDS             4631..5656
                     /locus_tag="PLU11_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPN59531.1"
                     /translation="MKIATVISFLEAWAPSSLQESYDNAGLITGSPEWDCTGIICSLD
                     ATEAVIREAKARGCNLVVAHHPIIFGGLKKINGKNYVEQTIIRAIKEDIAIYAIHTNL
                     DNVLSGVNGRIADQLGLLNQSILAEKDLTLKKLFTFVPVDHAEKVRTALFDAGGGQIG
                     NYSECSFNSEGTGTFRAGEGANPFVGQLNERHSEPEIKLELVFPAHLQGNMIRALRAA
                     HPYEEVAFDVMALANLHPAIGSGVIGELPEPLSEPEFLKKVKSVFGTPVIRHTAFSGQ
                     VVKRVAVCGGAGSFLISKALAKGADAYLTADMKYHEFFDANDRLLLADLGHYESEQLR
                     SGCWKRF"
BASE COUNT         1545 a         1177 c         1381 g         1590 t
ORIGIN      
        1 gatctcaaag ggtatattca taacccggcc tggtattttg catccgataa tgattctgta
       61 aaaatggcta cggatctgct gatgatgacc catggctggc gccgcttccg ctggcaggaa
      121 ctggcacaaa aagtaaaaca accgttaccc tttgttgatc cgtcttatat taccattacc
      181 ggaagagtga ccctggatgg caccaaccgg gcttttgggg agaaaaacct gctggcattg
      241 attactgccg agggaatgaa aaggaatgcg cagtttatca gtacggataa gcagggaaat
      301 tttaaactcg attcattact attctttggc agtgcccgga tctttatcct ggatatcaag
      361 ggccgtaaga gtcagtacat tgatgtgaaa atgaccagtg attcattggg tcgcccatac
      421 ccgatagcca ttcccggatc gtcagatttt gccacggtga atgtgaaaga aaaacagaaa
      481 cagctcgcgg aagattttga tgccatccag aaagccaatg gcattatgct ggaaggaatt
      541 acgcttaaag tgcggaagaa aagtccgacc gaagaactgg atgataagta tacccgtggg
      601 atgttcagtg gcatggctca gaaaaccttg gacctggtga acacggacca atttatttcg
      661 gagaataata ttttcgatta cctgatgtca cgggtaccag gtctggatgt aaccaatgaa
      721 ggggctgagt ataaagtgta ttaccggcag ggtccatcat tgagttcact gggtcctatc
      781 cccatgaccc tttacctcaa tgaaattgaa accgatgcca gtgtgattgc tactttgcct
      841 gctactgaaa ttgcgatggt aaaagtttac agccagtttg tcggggcagt cggtggaggt
      901 gccggaggtg ccatggccat ctatacccgc aaaggatctg atattgtcaa ttcctcccgc
      961 ggcgatatca tccgctatag tgggtttacc ctggtcaagg atttctttgc ccccaattac
     1021 aaaaccgacc cggccctgct gaagaaaccc gatacccgca tcaccatcga ctggcgcccg
     1081 aatattttcg tcaacaatat tgatccggtg attccgatca gtttctacaa cagcgaccgg
     1141 accaaacggt tccgcgttgt ggtggaaggg atgacgacgt cggggaagct gatatcgttg
     1201 gaggagataa tatctaagta aacccattat tttttttaaa aatttctaaa gcaatgttat
     1261 gagattccgc atgattgttt tggggttctt aatatgcggt tttaccggat catttttaca
     1321 tgcacaggac aatcttgcaa aaaacaactt catgtctgaa aaagtgcatt tgcatattga
     1381 caggtatgtt tttggtgcag gggaaacagt ctggtttaaa gcctacattc tgtcaggcga
     1441 caccagtgga ttaaccagtt ctgttttaca tgtcgaactg ctttttggag attctctgtt
     1501 aagctatcaa cgattgcccg ttgttctttc tgtaagcaat ggtcagtttt ttctagctga
     1561 cagtttagtg acagggtggt atactgtcag ggcatataca gaagcaatgt taaatcagca
     1621 gccgaacagt ttttttcaat cttcaatatt tattcatggg cgaaagaacg gtcaaaaatc
     1681 gcagacagat gtttttagag attcaatgct tgtttctttt tttccggagg gaggaaatct
     1741 tgtaaaagga ctggagtcga ccgtagcttt taaggcgact gatgaatggg gtttccccgt
     1801 tgatttttct ggagtcgtaa gggctgaaaa tggggactca atagctgttg ctaatacatt
     1861 taagtttgga atgggtgaat tcaaattaag gccttccatc ggacagcgat actatgtcag
     1921 tacatcagtt aaaggaagca ttattaagta ttacctgcct gaagtggttg atgagggagt
     1981 tgcgctttca atgatatatc acccggaggg tatttatttt gaattgaatc aggcgggtag
     2041 caaccggatg aaaaaagcct ccgttgttgt tggtcatatg tttcaaaaag aagtattcag
     2101 aagaaaaatc acagataagg aaaatacttt tcagggtgta cttaatacga aacaacttac
     2161 ttcaggtata cttgcagtta cggtattgaa tgatgatgga ttgccgctgg cagagcggct
     2221 gatatttatt aataataaag aataccggca ggcagtaaaa atcattgctg atactgttga
     2281 ttttcggtca aaagccagga attaccttaa gattgccctg acagatacag ttctgggtag
     2341 cctgtcatta tcgattacaa atgcagatta tggctatacc tcagtgcggc cggcatcgat
     2401 aatcagtgat ttattactga cttcagaact aaaaggggac atagtgaatc ctgactggta
     2461 ttttcaggag ggaaatgata gtgtatctac ggggcttgat cttctaatgc tcactcaggg
     2521 ctggcgtaat tataaatggg cgattcatga taaaaactca gacttgtcga agctgtataa
     2581 ggatccggca tatattacaa tacgtggccg ggcgctgctt aggggcacaa aatcacctct
     2641 ttcagattca cgattgatgg tagtgctttc tgctaaaggg ataacgaata atattcttct
     2701 gactgaaaca gataaacagg gctatttcac tattgattcg cttctttttt tcggcaaggc
     2761 aaggttttat tttgccgaac aacgcgaaaa aaagaaagcg aggtatattg atatcattct
     2821 ttcagaagga atttcttcag acaaacttct gccagagaaa agaaccaggc aactattagt
     2881 ggatcgccag catcagcatt atcaaaagat acctgacagt attatcaatt acctggagga
     2941 gatcacacgg gcagagggta agatgatgca aggggtaact gttacagcaa aaatgaaaac
     3001 tccggttgaa aaattggagg atgaatatac ggttggtgct tttagtacaa atgcttttgt
     3061 agagcgggtc attgatttgg ttaatacgaa tgaacctgtt gttcagccta atattttcga
     3121 gtatttaaag tcgcgtattc ctggactcat gattgcagat ccggacctga gtaaaagccc
     3181 cccggatcct tcacaacccg gttttgaccc gcggaatgat ccaacaaagc tgcggttatt
     3241 ttatcgtcaa atgcctagtg tcagcagcct tggaaatccg ccaatggtgg tatacctcaa
     3301 tgaaattgag accgatccgg atgtaatagc gacaatacct gccgctgaaa ttgcattaat
     3361 taagatttac agcagttttg tttcagctcc tggtgcaggc cctgggggtg taatggctat
     3421 ttatacaaaa aaaagtaagg atatgtctga tcccttggga agtactattt cttacgaagg
     3481 gttttcagtg attaaagaat tttttgctcc aaactataaa gctgatcctt cgataatttt
     3541 caaacccgat acccgcatca ccatcgactg gcggccgaat atttttgtca acaatattga
     3601 tcccgtgatc ccgatcagtt tctacaacag cgaccgcacc aaacgtttcc gcgtagtggt
     3661 ggaagggatg acgacgtcgg ggaagctgat atcaattgaa cagatcattt ctaagtaaat
     3721 ttttgcggaa tcttgttgca gaaaattatt gtttattaat tatttattat tgcgtgaaag
     3781 cagcaatagt aaaagcgggg agttgccttt ctttaaggta actccccgct ttctaataat
     3841 aaataattta ataattaata atggtctatt ccttggggaa tcccgctgcc gccttcacct
     3901 cattcagctg catcgtatgc cgttggctgt gtgcgccgat cagcagataa agctggtaac
     3961 agtccatcca gttggttcct aactgtacca cccggttgcg catgtcttct gtggaggtgc
     4021 gcatataccg gatatgagcc gtgcgcgtgt ctttgaagtc agccagggcc tcttctaaat
     4081 tcttatagcc ggtgtttttg ggttgaatgg gttccggcgc ctgattcttt ttagaacggt
     4141 ctttcaccat catcaccagc tcctcatcct ttaccttgat ctcggttctt ttttccgggt
     4201 taggggccgc tttcatagcg ttttcaaaca tgccccagag cattttttct gatgcggcaa
     4261 tatgaaatac acactccttc accgaccagc ggtcaggtgc ggccttaaaa tccagttggg
     4321 cttctgaaag acctttcgtg gctttcacca gatccttctg cgactccttc atcagggtga
     4381 tcgcatattt acggtcagat tttgaaatgg aattgttgac aatggttccg gcagatccgg
     4441 taatcaccag caggaggagt aacaggcggc cggtcgttct tctgagcatg gcaggtaaat
     4501 taagattaaa aaagcaatgc gttaacagtt cagtgggcag aagcgatgta aaaggtacta
     4561 aagtttttga atttcccgca taagcttttt cggctgttta tggtttaacg ctttgctgta
     4621 actttccgct atgaaaatag ccactgtcat ttcctttttg gaagcctggg cgccttcctc
     4681 actccaggag tcttacgata atgccggact gatcacgggc agccctgaat gggattgtac
     4741 cgggatcatc tgcagtctgg atgccaccga agccgtgatc cgggaagcca aagccagagg
     4801 gtgtaacctg gtggtggccc atcaccccat cattttcggg gggttaaaaa aaattaatgg
     4861 gaagaattac gtagagcaga cgattatacg ggccatcaag gaggatattg ccatttatgc
     4921 catacatacc aacctggata atgtgctttc cggtgttaat ggacggatag cggatcagct
     4981 tgggctgttg aatcaaagta tactggctga gaaagacctg accctgaaaa agctattcac
     5041 ttttgtaccg gttgaccatg cggagaaagt cagaaccgcc ctgttcgacg ccggcggcgg
     5101 ccagatcggt aactatagcg agtgtagttt caattcagaa ggcacaggca ctttccgggc
     5161 tggggaagga gccaatccct ttgttggtca gctaaatgag cggcatagcg agccggaaat
     5221 caaactcgaa cttgtgtttc cggcccacct gcagggcaat atgatccggg ctctccgggc
     5281 tgcccatccc tacgaggaag tggcttttga tgtgatggcc ctggccaatc tgcatcccgc
     5341 gatcgggtcc ggtgtgatcg gtgaattacc ggagccgctg tcggagccgg aatttctgaa
     5401 aaaagtcaaa tctgtttttg gtaccccggt aatccggcat accgcctttt cgggacaggt
     5461 tgtgaaaagg gtggcggttt gcggaggagc gggtagtttc ctgatatcca aagcattagc
     5521 aaaaggggca gatgcttacc tgacagccga tatgaaatat cacgaattct ttgatgccaa
     5581 tgaccggctg ctcctggcag atctggggca ttatgagagc gagcaattac gatcgggttg
     5641 ctggaagagg ttttagccaa aaaattccct acctttgccg tccttaaaac tga
//
LOCUS       DAOPMW010000209         5669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16466_length_5669_cov_10.550766, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000209 DAOPMW010000000
VERSION     DAOPMW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16466_length_5669_cov_10.550766"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLU11_10170"
     CDS             <1..674
                     /locus_tag="PLU11_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59532.1"
                     /translation="IRNQQYIHPTINYRRTYMETLKKIMTGSILLFAVLIVSAQSESK
                     WQAAFNESYTQEANKQYSAAINSLKAVYDEKSYELNLRLGWLTYLNKEYTTAQSYYQK
                     AVNLRPYSLEAKFGLIQPLAALQLTDKTLQVYEDILKIDPQQTKANYWVGVIWYNRKK
                     YETAARYFEKLINLYPFDYDGNHMLGWTYLNMGRSNDAKLLFGKALLAKPGDASCLEG
                     LKQVR"
     gene            complement(711..1490)
                     /locus_tag="PLU11_10175"
     CDS             complement(711..1490)
                     /locus_tag="PLU11_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPN59533.1"
                     /translation="MKATVPAKTPDQYIAGFPAAVKKRLEEIRKTIRDNAPDAVEGIS
                     YGMPGFKYLGKPLVYFAGYENHIGFYATPTGHEAFKKDLAGYKTGKGSVQFPLTEPLP
                     LALIKKIVQFRVKENEGMAAAKKKITATPVKKKATPADKEAGQVKQWLDKQEPAMRKE
                     IDVVRKLIKKAAPALNERIKWNAPSYYTTVDLFTFGPYKNKRILLVFHHPAIVEINSP
                     LLEGEYKDRRLVYFNSSKEAVKNEKELSRIIRLLTKKAGNL"
     gene            complement(1509..2033)
                     /locus_tag="PLU11_10180"
     CDS             complement(1509..2033)
                     /locus_tag="PLU11_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN59534.1"
                     /translation="MIFLYILAAIIAAFFLTALLVKKDFSLEKQIVINKTQKEVFDYL
                     KMIRNQEKYSVWVMRDPKINLVYSGTDGTVGFKSAWTSNDKNTGIGEQEIIKLTEGVS
                     TEVEIRFKKPFEATNWAKTTVTAVSEHETRVHQLFYGRSKFPMNIMNLFMDKLLGKDM
                     LQNLVNMKKNLEEN"
     gene            complement(2075..2452)
                     /locus_tag="PLU11_10185"
     CDS             complement(2075..2452)
                     /locus_tag="PLU11_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPN59535.1"
                     /translation="MKKQTILILYWVFTLLFSAFMIWSSVPGINPKGEVLQFMNGYLG
                     YPVYFIHFISMAKIIGSVVILIPGLNKIKEWAYAGLFFDLAGAVFSIYMVQKKFDAGM
                     LFILVPVLLGAASYYFWNKRNAL"
     gene            complement(2606..3454)
                     /locus_tag="PLU11_10190"
     CDS             complement(2606..3454)
                     /locus_tag="PLU11_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN59536.1"
                     /translation="MSNNIHPCLWFNGNAKEAAEFYCSLFPESRITFDTPMVVNFYLH
                     GQKFMGLNGGPMFKFNPSVSMFVTSENNEEIDALYAKLIEGGMVMMPLDKYEWSDYYA
                     FVQDKFGLAWQLYKGSYSAVNQKITPCFLFTDDHFGKAEAAVNYYTALFPSSSINGIL
                     LYQPDEGEQVAGKVKHSQFIIDDKVFMAMDGAGNHGFSFNEAISFVVECKDQEEIDKY
                     WNQLTANGGAESMCGWLKDPFGVSWQIIPAKMGQIMTDSGNGAGAMKALMQMKKIVIA
                     ELESSR"
     gene            complement(3595..4032)
                     /locus_tag="PLU11_10195"
     CDS             complement(3595..4032)
                     /locus_tag="PLU11_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN59537.1"
                     /translation="MARVSTYLNFARNTEEAFLFYKDAFKTEFIGPIMRFGEVPPQPG
                     APPMAEADKNLVMHVELPVTGGHILMGTDAPESWGFTLNFGNNAHIMLEPDSREECDR
                     LFNALSVGGKVSMAMQDMFWGSYFGSFSDKFGVMWMVSHTPQK"
     gene            complement(4260..4679)
                     /locus_tag="PLU11_10200"
     CDS             complement(4260..4679)
                     /locus_tag="PLU11_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN59538.1"
                     /translation="MSTPTRITVEAVISAPVEKVWDYYTGPEHIMQWNQASPDWHCPA
                     STNDLRPGGKFSSTMAAKDGSFSFDFWGIYDEVDTHKKIVYTMGDGRKADVLFTKNEN
                     DTHVAVIFEPESENSLELQQEGWQAILNSFKIYTETH"
     gene            4792..>5669
                     /locus_tag="PLU11_10205"
     CDS             4792..>5669
                     /locus_tag="PLU11_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPN59539.1"
                     /translation="MKHVCILVPETAVIEAVADPRYLFSAVNQFLAAAGRAPLFEVKL
                     VAARRETRLDNAAFSVHADLLLDEVEHTDLVIIPALSGNLSAAIDANKALIPWIIEQY
                     NKGAEVASLCLGAFLFASTGLLDGKKCSTHWGFCNEFRAMFPKVEMVDGSIITDEQRL
                     YSSGGANSYWNLLLYLVEKYTDRPTAILAAKYFAVDIERDSQAAFMLFNGQRAHEDDT
                     IKQVQAYLEAHYTNKVTVDELAERFAIGRRSFERRFKKATNNTVVEYLQRVKMEAAKR
                     SFETSRKNISEVMFDVG"
BASE COUNT         1516 a         1216 c         1353 g         1584 t
ORIGIN      
        1 ttatacggaa ccaacaatac attcacccaa caatcaatta caggaggact tatatggaaa
       61 ctttaaagaa aataatgacc ggcagtatac tgttattcgc cgtattgata gtcagtgccc
      121 aatccgaaag caaatggcag gcggcattta atgaaagtta tacgcaggaa gcgaacaagc
      181 aatatagtgc tgcgatcaat tcgctgaaag cggtatatga cgagaagagt tatgaactga
      241 atctccggtt gggctggctc acctatctga ataaggagta taccacagcg cagtcctatt
      301 accagaaagc cgttaatcta cggccgtatt cactggaggc gaagtttgga ctgatacagc
      361 cgctggccgc gctgcagctg acagataaaa ctttacaggt gtatgaggat atattaaaaa
      421 ttgatccgca gcagaccaag gccaattact gggtgggcgt gatctggtac aaccggaaga
      481 agtatgaaac ggctgcccgg tattttgaga aactcatcaa cctctatccc tttgactatg
      541 atggcaatca tatgctgggc tggacctatc tgaatatggg gcgaagcaat gatgcgaagc
      601 tgctttttgg taaagcttta ttggctaagc ccggggatgc ttcttgtttg gagggtttga
      661 agcaggtgag gtaagtttta agttataagt aaagaactgt ttgttatgcg ttagaggttt
      721 ccggcttttt ttgttagaag gcggatgatg cgggacagtt ctttttcatt tttaacggct
      781 tctttgctgc tgttgaaata caccagtcgc cggtccttgt attctccttc cagtaaaggg
      841 gagttgattt ccacaatcgc cggatggtga aagacgagca ggatgcgttt gtttttgtat
      901 ggaccgaaag tgaataaatc gacagttgtg taataactgg gggcattcca tttgatccgt
      961 tcattcagcg cgggggctgc ttttttgatc agtttacgga cgacatcaat ttccttacgc
     1021 atggcgggtt cctgtttatc cagccattgc tttacctggc cagcttcttt atctgctgga
     1081 gttgcttttt ttttaacggg agttgcggtg attttctttt tggcagcggc catcccttca
     1141 ttttccttta ccctgaactg tacgatcttt ttgatcaggg ccaggggcag gggttctgtc
     1201 aatggaaatt gtaccgaccc tttaccggtt ttataaccag ccagatcttt tttgaaagct
     1261 tcatgtccgg tgggcgttgc ataaaatccg atatggtttt cataaccggc aaagtacacc
     1321 agcggtttac ccaggtattt gaaaccgggc atgccataac tgatcccttc tactgcatcc
     1381 ggggcattgt cacgaatggt tttacgtatt tcttccagtc gttttttaac cgcggccggg
     1441 aaaccggcta tatactgatc aggtgttttg gcaggaactg ttgctttcat aaaataaaat
     1501 tgaaaagttt aattttcttc caggttcttc ttcatattca ccaggttttg cagcatatct
     1561 tttcccagca gtttatccat aaagaggttc atgatattca tgggaaactt gctgcgtcca
     1621 tagaataatt ggtgtacccg ggtttcgtgt tcgctcaccg cggtcactgt cgttttcgcc
     1681 cagttggtgg cttcaaaggg ttttttaaat cggatttcca cttcagtgct gacaccctct
     1741 gtcagtttaa tgatttcctg ttccccgatc cctgtatttt tatcattact ggtccaggct
     1801 gatttgaatc caaccgtgcc atcggtgcct gaatacacca gattaatttt ggggtctctc
     1861 atcacccaga cactgtattt ttcctggttc cggatcattt tcaggtaatc gaatacttcc
     1921 ttctgtgttt tgttgatcac gatttgtttt tcaagggaga aatctttttt taccagcaaa
     1981 gcggttaaga aaaaagcagc gatgatggcg gcgaggatat agaggaagat catggttggg
     2041 ggattggggg attaagaaat agggattggg gtgatcagag ggcattcctt ttgttccaga
     2101 agtaatacga agcggctccc aggagtaccg ggacaaggat gaataacatg ccggcatcga
     2161 attttttctg taccatataa atagagaaaa cggctccggc cagatcaaag aaaagtccgg
     2221 cataagccca ttcctttatt ttattcagtc cgggtatcag gatgacgacc gagccgatga
     2281 tcttggccat actgataaaa tggataaaat agaccgggta cccgagatag ccgttcatga
     2341 actggaggac ttcgcccttg gggttaatgc ccggcacaga ggaccagatc atgaaggcag
     2401 agaagaggag ggtgaagacc cagtataaaa tgaggattgt ttgttttttc ataattatag
     2461 tttttaagaa ccaagaacca agaaccaaga accaagatgc caagacgcca aagaagcatc
     2521 aatgtacaag atccaaagaa aaatcaatat tcaaatctca aagccttcta aggaggtttt
     2581 attgagattt gggaattact atttactaac gactactctc taattccgct atcactatct
     2641 tcttcatttg catcagcgcc ttcatcgctc cggcgccatt tccggaatcc gtcatgattt
     2701 gtcccatttt agcggggatg atttgccagg atacaccgaa gggatctttg agccagccgc
     2761 acatgctttc ggcaccacca tttgcggtga gctgattcca gtatttatct atttcctcct
     2821 ggtctttgca ttccactaca aaggagatgg cttcattaaa tgagaatccg tgattaccgg
     2881 caccatccat ggccatgaag actttgtcat caatgataaa ctgggaatgt tttactttcc
     2941 cggctacctg ctcaccctca tcaggctggt acaacaggat tccattgatg gaggaagaag
     3001 ggaaaagtgc ggtataatag ttcaccgcag cttctgcttt tccaaaatga tcatctgtaa
     3061 ataagaagca gggcgtgatt ttttgattga cggcgctgta acttccttta tataattgcc
     3121 aggccagccc gaatttatcc tgtacaaaag catagtagtc actccattca tatttatcca
     3181 ggggcatcat gaccataccg ccctctatca gtttcgcata gagtgcatca atctcttcgt
     3241 tgttttcact ggtgacaaac atggaaacag aaggattgaa tttaaacatc ggtccgccat
     3301 tcagtcccat gaacttctgt ccatgcaggt agaaattcac caccatgggt gtatcaaacg
     3361 taatacggga ctccgggaac aggctgcagt agaattcagc ggcttcttta gcattgccgt
     3421 tgaaccagag gcaggggtgg atattgttgg acatgttgaa aagttaacaa gttaaaagtt
     3481 tataagaaca tctataaagt acgaggtacg aagtacgaag tacgctgacg ttctatgaag
     3541 aactgcttct tagaacgggc tgtttcctga cacctgacac ccgacatctg acacctattt
     3601 ctgcggtgta tgactgacca tccacatcac cccaaactta tcgctgaaac tgccaaagta
     3661 ggagccccag aacatatcct gcatggccat ggagactttt ccgccaacgg aaagcgcatt
     3721 gaacagccgg tcacattctt cccggctatc gggttcgagc atgatatggg cattgttgcc
     3781 aaagttcagg gtaaaacccc aggactccgg tgcatcggtg cccatcagaa tatgtccgcc
     3841 ggtgacaggt aattccacgt gcatcacgag gttcttatcg gcttccgcca ttgggggagc
     3901 accgggttgc ggcggtactt ctccgaagcg catgattggt ccgataaact cagttttaaa
     3961 agcatcctta taaaaaagga aggcttcttc cgtattacgg gcgaagttga ggtaagtgga
     4021 gacgcgggcc atatgaggtc gtttaaaagg tgaattattt aatataaagt acgagacact
     4081 taattcaaag tacgaggtac gaagtacgaa cagcattccg taaaacgagt aactgtttat
     4141 tattatagat tcatatccta aggaggggct gttggctacc gactaccgac tacgaactac
     4201 cgactagttt caggggctgt ttcctcacac cagacagctg acacccgaca ccttcttcct
     4261 taatgcgttt ccgtataaat tttaaaacta ttcaatatcg cctgccaccc ctcttgctgt
     4321 aattcaagtg aattttcaga ttcgggttca aagatgacgg ctacatgggt gtcattttca
     4381 tttttagtaa ataaaacatc cgctttgcga ccatcgccca tggtataaac aattttttta
     4441 tgggtgtcca cttcatcata gatgccccag aagtcgaaac tgaagctgcc gtctttggcg
     4501 gccatggtgg aagagaactt accacccgga cggagatcgt tcgtggaagc cgggcaatgc
     4561 caatcgggac tggcctggtt ccattgcatg atatgttcag gacctgtata atagtcccat
     4621 actttttcga ccggagctga aatcacggct tcaaccgtga tacgtgttgg agtagacatg
     4681 ttggtttttg ttttgcaatg taaacttact gagattaaac aaaagggatg accggtaaaa
     4741 acgacaattc aaagggttga ttgcgacaac cgtttttaat acctttaaaa gatgaaacat
     4801 gtatgcatcc ttgtacctga gacagcagta attgaagcgg tggctgatcc ccgatacctg
     4861 ttttccgctg taaatcaatt cctggcagca gccggaagag cgcctttatt tgaagtaaaa
     4921 ctggtggcgg cccgcaggga aacccgactc gacaatgctg cattctccgt gcatgcggac
     4981 ctgttgctgg atgaagtgga acataccgac ctcgtgatca tacctgcttt gagcggtaac
     5041 ctgtcagccg ctatcgatgc gaacaaagcg ctgatcccct ggattattga acaatataat
     5101 aaaggggcgg aggttgcttc actttgcctg ggtgcttttc tgtttgcctc caccgggttg
     5161 ctggatggaa aaaaatgttc tactcattgg ggcttttgca atgagttcag ggccatgttc
     5221 cctaaggtgg aaatggtgga tggcagtatc atcaccgatg agcaacgact gtatagcagc
     5281 gggggtgcta attcttactg gaacctttta ttatacctgg tagagaaata tactgatcgt
     5341 ccaacagcga tactggccgc caagtatttt gcggtggata ttgagcggga cagccaggcg
     5401 gcttttatgt tgttcaacgg acagcgggcg catgaagacg atacgatcaa gcaggtgcag
     5461 gcttatcttg aagcgcatta taccaataaa gtaacggtgg atgaactggc cgaacgattt
     5521 gcgattggcc ggaggagttt tgaaagaaga tttaagaaag ccacgaacaa tacggttgtc
     5581 gaatacctgc aacgggtaaa aatggaagct gccaaacgga gttttgaaac cagccgtaag
     5641 aatatcagtg aagtgatgtt tgatgtggg
//
LOCUS       DAOPMW010000210         5638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16608_length_5638_cov_7.898442, whole genome shotgun sequence.
ACCESSION   DAOPMW010000210 DAOPMW010000000
VERSION     DAOPMW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16608_length_5638_cov_7.898442"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..1489)
                     /locus_tag="PLU11_10210"
     CDS             complement(263..1489)
                     /locus_tag="PLU11_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59540.1"
                     /translation="MKKTLPVILLSLAIYCSKPVSAQSTAITLDVVSWNMEWFGYPPS
                     GPSNEDLQEANAKKILRYLNADLYGMVEVVDSMRFRRLVDSLGNNEYGFFISPFCSNN
                     STGTGNSWLSGQKLAFIYRKSIFSNIRTRGLLRSSVSANTNWASGRFPYMFSATATIN
                     SVSKDINFIVIHGKAGSTANDYDRRKAGAQELKDTLDAQFANTINIIIGDYNDALNTT
                     ISTGSGPASSFQPIIADSTDADHYKSITLPLGVAGQSSMINFPNVVDNHVISNELTPY
                     YVLNSAKIRNDITAVVPDYITTHNTSDHYPVFSQFSLEGLITGIPVVTPTAFGIQVSP
                     NPFGSSLRVTATKSLANVQVRLLNMQGQIIHTKSLGFLSAGSSYEPSFTDLPRGIYFM
                     QVETKQQRTVVKLTHL"
     gene            complement(1559..3262)
                     /gene="recJ"
                     /locus_tag="PLU11_10215"
     CDS             complement(1559..3262)
                     /gene="recJ"
                     /locus_tag="PLU11_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPN59541.1"
                     /translation="MAIEKRWKILEADAVKAAALQQALTIHPSLCSILVQRGIETFEE
                     AKAYFRPRMAELHSPWLMKDMEKAVDRILAAVGVEEKILVFGDYDVDGTTAVASMYQF
                     LHKFHQPLDFYIPHRYREGYGVSKAGIDFAHENGFTLIISLDCGIKSADLIRYAAGLG
                     IDFIVCDHHLPDEAIPPAVAILNPKQVDCSYPFKELCGCGVGFKLMCALAERMELPPE
                     TAYDHLDLLATAIAADIVPMYGENRILAFHGLIKANLQPNFGIKALATLSGVVKSLHI
                     TSLVFMIAPRVNAAGRMDDARKAVQMFVAETETEALHFAEQLHSDNSDRKEADKSITE
                     EALQTIRANEAWLNRKSTLVYQPHWHKGVVGIVASRLIEHFYRPTIVLTRSGDYVAGS
                     ARSVPGFNLYEAIHACREHLLGYGGHFAAAGMTLETGQVDAFREKFESVVSSTIEPRL
                     LIPEIVIDAVISLKDIRPSIYSILCQMEPFGPENLRPIFLARRLLNTGYSKIVKEEHI
                     RFSLKQDQVTLTGIGFGMAAKFSLLEQGSPVDVVFTIDENEWNDTKTLQMRVIDFRAS
                     E"
     gene            complement(3289..4251)
                     /locus_tag="PLU11_10220"
     CDS             complement(3289..4251)
                     /locus_tag="PLU11_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPN59542.1"
                     /translation="MQLPTDLIQDELTIFEQRFKEAVRSNAPLLDRIMRYVVKRKGKQ
                     LRPMFVLLSAKLCGPISDPAYRAASLVEILHTATLVHDDVVDDSMERRGFFSPYALWK
                     AKANVLIGDYLLAKGLLLSLDNHDYQILHILSDAVRKMSEGELLQLEKARSLNLKEDI
                     YYEIIRNKTASLLASACAAGAWSASQDEGITEKMRQFGEKTGMAFQIKDDLFDYASED
                     VGKPTGNDIKEKKLTLPLIYTLNNTDKETRRKIIYIVKNNNQDKTKVKWVIDQVTAAG
                     GIRYATEKMNAYKKEALDILHSFPESPYRKGLEDMVLFVTDRKY"
     gene            4446..>5638
                     /locus_tag="PLU11_10225"
     CDS             4446..>5638
                     /locus_tag="PLU11_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59543.1"
                     /translation="MASKKYLFLAVSFCLLASYGFSQRVGTSYDVKDSSLVPGKRMPQ
                     HTEFLNGSYNFPSKPRNQWEIGVKGGLFQVSGDIPAQFVSPGFGAHVRKAFGYIFSLR
                     LEYMYGIGKGYSFRAAQNYAKNPAWGANIADPNQRYSAPQQINGVGTPLIVSSKTRLT
                     TTPLEQVFYNYKTKVQDLSLEAVFTLNNVRFHKSKTGFNIYGFAGVGGSIYDTKVNSL
                     DEDANNQKYNFNSITQASYKNRRDFIKDMKKNILDGSFETAAENQGERRPKLFGDTFK
                     PSGTVGMGIAFKLSNRINLALEDRWTIIKDDLLDGQRWQEQPWGDATLTRDFDSYNYM
                     TVGLNINLGAKSVEPLWWLNPLDYAYSEIRNPRLMRLPKPVLPDTDADGVTDQFDREQ
                     TPAGCP"
BASE COUNT         1439 a         1305 c         1356 g         1538 t
ORIGIN      
        1 aacagccctt tctgaaggtg ttagctgttc gttgttagct gtcaggaaac agccctttct
       61 gaaggtgtta gctgttcgtt gttagctgtc aggaaacagc cctttctgaa ggtgttagct
      121 gttcgttgtt agctgtcagg aaacagcccg ttctcagaaa gttcctctaa actctccttt
      181 aagtctcctt ctgctttagt acctcctaaa ccttctaaac gctcctgtaa atctttttct
      241 tctatcagat gccctaaacc ctctacaggt gtgtcagttt aactactgtt ctctgttgtt
      301 tcgtttcgac ttgcatgaaa tatattcccc gcggcagatc agtaaagctg ggttcatagc
      361 tggaacccgc actcaggaaa ccaagtgatt ttgtatggat gatctggccc tgcatattca
      421 gtaaccttac ctgtacattg gccagtgatt tggtggctgt tacacgaagg gaggacccga
      481 atggattcgg gctgacctgt ataccaaatg cagtgggtgt taccacgggt attccggtaa
      541 tcagtccttc caggctgaac tgcgaaaaaa cggggtagtg gtctgaggtg ttatgtgtgg
      601 tgatatagtc cgggacgaca gccgtaatat cattcctgat tttagccgag tttaatacgt
      661 aataaggggt aagttcattg gagataacat gattgtccac cacattcgga aaattgatca
      721 tactgctttg cccggccacc cccaatggca gggtgattga tttataatga tcggcatccg
      781 tactatcggc aataatgggc tgaaaagaag aggccggacc tgagccagtg gagatggtgg
      841 tattcagcgc gtcattataa tccccgataa taatattgat cgtattggca aactgggcat
      901 cgagtgtgtc tttcagttcc tgtgcccccg ctttgcgtcg gtcataatca tttgcagtgc
      961 tgcccgcttt tccatggatg acaataaaat taatatcctt gctcacactg ttgatggtgg
     1021 cagtggcaga aaacatataa gggaaacgtc cggaagccca attggtattg gcactgacac
     1081 tgctgcgaag taatccacgg gttctgatat tggaaaaaat ggatttacgg taaatgaaag
     1141 cgagtttctg tccgctcaac cagctattgc ccgtacccgt gctgttgtta ctgcaaaagg
     1201 gagaaataaa aaagccatat tcattatttc ccaatgaatc caccagccgg cgaaaccgca
     1261 tgctgtccac cacttccacc atcccgtata aatcggcatt caggtaacgg agaatcttct
     1321 ttgcattggc ttcctgcaga tcttcgttgg aagggcctga tggagggtaa ccaaaccatt
     1381 ccatattcca gctgaccaca tccagggtaa tggccgtact ttgagctgat accggtttac
     1441 tgcagtaaat cgcgagggac aggaggatga cgggtaacgt ttttttcatg cgaaacattt
     1501 caacggcaaa tgtaagtcca atttgcagaa ccccgttgta cgaatggttg cttacgtact
     1561 attccgatgc acgaaagtcg atgacacgca tttgcagtgt tttggtgtcg ttccattcgt
     1621 tttcatcaat cgtgaatact acatctaccg gggaaccctg ttccagcagg gagaatttag
     1681 ccgccatgcc aaaaccaata cctgtcaggg tgacctggtc ttgttttaaa gagaaacgga
     1741 tatgttcttc ttttacaatt tttgagtatc cggtatttaa aagtcgtcgg gccaggaata
     1801 tgggacggag attttcgggt ccgaaaggct ccatctgaca gagaatagaa taaattgaag
     1861 gacggatatc tttgagtgag atgacggcat caattacaat ctccggaata agcaaacggg
     1921 gttcaatcgt tgaggatacc accgattcaa atttttcacg aaaggcgtct acctgaccgg
     1981 tttccaacgt cattccggcg gctgcaaaat gcccgccata gcccagtagg tgttcacggc
     2041 aggcatggat cgcttcatat aaattaaacc ccggtacact acgggcactt ccggctacat
     2101 agtcgccgga gcgggtgagc acaatggtcg gccggtaaaa atgttcaatc agccgggaag
     2161 ccacaatgcc caccacccct ttatgccagt gaggctggta tacaagggtg gatttccggt
     2221 tcagccaggc ttcattggca cggatcgttt gcagggcttc ttctgtaata gacttgtccg
     2281 cttcttttcg gtcggaatta tcactatgta attgttctgc aaaatgaagg gcttctgttt
     2341 ccgtttcggc tacaaacatc tgtactgctt tccgggcatc atccattcgt ccggctgcat
     2401 tcacgcgtgg ggcaatcata aataccaggc tggtgatatg taatgatttt accacgccac
     2461 tcagggtagc taatgccttt atgccaaaat ttggttgcag gttggccttg atcagtccgt
     2521 gaaaagccag gatcctgttt tctccgtaca tgggaacgat atcggcagca atggcagtag
     2581 ccagcagatc gagatgatca taggcggttt ctggtggaag ttccatccgt tcggccaggg
     2641 cacacatcag tttaaagccg accccgcaac cgcataattc tttaaaagga tacgagcaat
     2701 caacttgttt ggggttgagg atggctacgg ccggtggaat cgcctcatcg gggaggtgat
     2761 ggtcgcagac aataaaatca atacccaggc cggcagcata acggataagg tcagctgatt
     2821 ttatgccaca gtccagtgaa atgatcaggg taaatccgtt ttcatgggca aagtcgattc
     2881 cggctttgct gaccccatac ccttcgcggt aacggtgcgg gatataaaaa tcaaggggct
     2941 ggtggaattt gtgcaaaaac tggtacatac tggccacggc ggtggtacca tccacgtcat
     3001 aatccccgaa gaccaggatt ttttcttcga ctcctacggc ggccagtatc cggtctaccg
     3061 ccttttccat atccttcatc agccagggag aatgcagttc ggccatccgg ggcctgaaat
     3121 aagctttggc ttcttcaaag gtttcaatcc ctcgttgtac cagtatactg cacagggaag
     3181 ggtgtatcgt taatgcctgt tgcagtgcag ccgcttttac agcatccgcc tccagaattt
     3241 tccatctttt ttctatggcc attgggtaat taggtttggc accgataatc aatatttccg
     3301 gtcagtaacg aacaggacca tatcttccag tcctttgcgg tagggacttt cggggaaact
     3361 gtgcaggata tcaagggctt ctttcttata cgcattcatc ttttcagtgg catatcggat
     3421 accacccgcg gcggttacct ggtcgatcac ccatttcact tttgttttat cctggttatt
     3481 gtttttcaca atataaatga tctttctccg ggtttcctta tcggtattat taagggtata
     3541 gatcaacggt agtgtcagct ttttttcctt gatatcattg ccggtgggtt tgcccacatc
     3601 ctcactcgcg tagtcgaaaa ggtcgtcttt tatctggaaa gccataccgg ttttttcccc
     3661 gaactggcgc attttttcgg ttataccttc gtcctggctt gccgaccagg cgccggcggc
     3721 acaggcagaa gccagtaaag aagccgtttt attccgtata atctcgtaat aaatgtcctc
     3781 tttcaggttc aggctccggg ctttttccag ttgcaggagt tctccttcac tcattttccg
     3841 gacggcgtct gacaaaatat gaaggatctg gtaatcgtga ttatcgagag aaagcagcaa
     3901 tcctttggcc agcaggtagt cgccaatcag cacattggct ttcgctttcc agagggcata
     3961 aggagagaaa aatccacgcc tttccatcga atcatcgacc acgtcgtcgt gcacaagtgt
     4021 cgcagtatga aggatttcca ccaaagaagc ggcacgatag gccgggtcgg aaatgggccc
     4081 gcagagtttg gcggagagca gtacaaacat gggccggagc tgctttcctt tacgttttac
     4141 cacataccgc atgatccggt ccaggagggg tgcattgctg cgtacagcct ctttgaatcg
     4201 ttgctcaaaa atggtcaatt cgtcctggat gagatcggtg ggtagttgca tgccaaaaaa
     4261 aagcaattta cgggcaaaaa aataattgtt tgcagatgca acaaaaaagg gtcaaaaatt
     4321 gactaagtta ttgaaaaata aagggcaaaa gggtaaataa atgagttaaa aatttggtat
     4381 ttagtcctca tttaatattt ttgcgtatta tttaggacat tgttcacaaa tcttaatcct
     4441 tagttatggc aagcaaaaag tacttatttc tggcggtcag tttctgtctg ctggcgtctt
     4501 atggtttttc acagagggtg ggaaccagtt acgacgttaa agattcttcc cttgtacccg
     4561 gtaaacggat gccgcagcat accgagtttc tgaacgggtc ttacaatttt ccttcaaaac
     4621 cccgcaatca gtgggagatt ggtgtgaaag gtggcttatt ccaggtgagc ggtgatatcc
     4681 ccgcacaatt tgtttctccg ggattcggag ctcacgtaag aaaggctttc ggatatattt
     4741 tctcgctgcg tttagagtac atgtatggta ttggaaaggg ctacagtttc agagctgccc
     4801 agaactatgc taagaacccg gcatggggtg caaatattgc tgacccgaat cagcgttatt
     4861 cagctccaca gcaaattaac ggggtaggta caccgctgat tgtttcttca aaaacaaggc
     4921 tgactaccac accgcttgag caggtgttct acaactacaa aaccaaggtt caggatctga
     4981 gcctggaagc agtgttcacc ctgaacaacg tacgttttca caaatccaaa accggattca
     5041 acatctacgg ttttgccggt gtgggtggtt ctatctacga caccaaagtg aattcactgg
     5101 atgaagatgc aaacaaccag aagtacaact tcaattccat cacacaggct tcttacaaga
     5161 accgtcgtga tttcatcaag gacatgaaga aaaacatcct ggatggcagt ttcgaaacag
     5221 ctgctgaaaa ccagggagag cgtcgtccga aattattcgg cgatacgttc aaaccttccg
     5281 gaacagtagg tatgggtatt gctttcaaac tgagcaaccg tatcaacctg gcactggaag
     5341 accgctggac catcatcaaa gatgatctgc tggatggtca acgttggcaa gagcagccct
     5401 ggggcgatgc tacactgacc cgtgattttg attcttacaa ctacatgacg gttggcctga
     5461 acattaacct gggtgctaaa tctgttgagc cactgtggtg gttgaacccc ctggattatg
     5521 cttacagcga aatccgcaac ccccgcctga tgcgtcttcc caagcctgta ttacctgata
     5581 cagatgcgga tggtgtaaca gaccagttcg accgcgagca aacccctgct ggttgccc
//
LOCUS       DAOPMW010000211         5630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16649_length_5630_cov_11.747265, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000211 DAOPMW010000000
VERSION     DAOPMW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16649_length_5630_cov_11.747265"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLU11_10230"
     CDS             <1..263
                     /locus_tag="PLU11_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59544.1"
                     /translation="KIITYDASSFVANKEKAEALFNQYVAKLESCGTLQDRQVAQTKN
                     LITLSYSARVNNRAKEVSLKLMDLGEGYVITVNVTDKYAKLN"
     gene            294..>1326
                     /locus_tag="PLU11_10235"
     CDS             294..>1326
                     /locus_tag="PLU11_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59545.1"
                     /translation="MKGILFFFTMLCCCSISQAQDSLGVYVRVKPEAVIFDDYKLEHD
                     PNYRKAMAQRGKPYASAAVRISEQMNSFLKGYPQKFDIGNYDARMDTIQKYLPGYHAF
                     IVASWNKKCSDYSSIPENTPLILVEIPLEYNDFFPFELQPYPRRFFVLLSPASVEKQS
                     EVAYTTFKAAYKKRKEPVVVKKEPTPPPVIKTTEPVVVAPAPVVKPAPPKPAATVDYN
                     PPTGTAFKADGKNEGYFQHKRKMDRFYVTNIRTALVNVNFSNQEYFKSKLVGVAGYSY
                     IDMSTFSYMNESGFYPPGIRTPALREANGKLAANYNCYMVENLVDNNNTMYDLLWVPY
                     SENLGMPKDM"
     assembly_gap    1327..1426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1585..2064
                     /locus_tag="PLU11_10240"
     CDS             1585..2064
                     /locus_tag="PLU11_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59546.1"
                     /translation="MKKVLFLFCLICLSVSMHAQQPVLQAPSLNRMLDLLTRDSVSYF
                     RGEEKSDPMNGTYHVSTLPAMGKYVINISESPQQPIPFSFFIEVPVSKAVTFAKIKAE
                     LVALTKAKKLKYKMVVKENYDERILALGDGVLIIQIHDSMGEGGNIRIAVFRDEKYE"
     gene            complement(2088..3164)
                     /gene="paaK"
                     /locus_tag="PLU11_10245"
     CDS             complement(2088..3164)
                     /gene="paaK"
                     /locus_tag="PLU11_10245"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HPN59547.1"
                     /translation="MSLHFHRLTVKEVKKETPECVSVLFDVPETLQNDFQFSQGQSLT
                     LRTTINGEEIRRNYSICSSPLENKLKVAIKKVEGGLFSSFANELLKKGDVLDVMPPVG
                     KFNTPLHPENKKNYLAFAAGSGITPVISLIKTTLATEKHSRFTLVFGNRNRNSIIFFE
                     ELEALKNQYMERFSLVHILSRERMDTDLNFGRISQEKLEELDRLEDFTGTDEFFICGP
                     TEMIFTVKAFLEKKGIAGNKIHLELFNSTKSRQSTVDSPQINTAGAVSNIDVKVDGRS
                     FEVTIPFNSDQTILDAALQQGADLPYACKGGMCCTCKAKLLEGEVSMDVHWGLEEEEV
                     AKGYILTCQSHPKTEKVVVDFDAK"
     gene            complement(3166..3759)
                     /locus_tag="PLU11_10250"
     CDS             complement(3166..3759)
                     /locus_tag="PLU11_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN59548.1"
                     /translation="MAKAQRKKASKKGLILKKAAAMFREKGFAATSMRDLAEAVGIEA
                     ASLYNHIRSKNEILEAICFDVANVYNTTIDTIEASNQKSIAKIDQLLRFHIRQMTENY
                     EDVYVSDREWKHLDEPYLSNFQTQRRNYRKKFASIIEEGIAKNEIRKIDAPTAVLIML
                     HAVSGIESWHRSTAKISADELENNMVMIMIDGLRKQG"
     gene            complement(3810..4394)
                     /locus_tag="PLU11_10255"
     CDS             complement(3810..4394)
                     /locus_tag="PLU11_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59549.1"
                     /translation="MKQNRFFCRLLLILVLLVTLDANAQFDSSFVKSQITKCADSLAY
                     GFRNRNWEVFARYSNPAMIGSLGGKEEFIRTIASMFGDISPDAWKLYKPGLVLQVIKS
                     GADLQCMIEMNSIIETDGKRITSKSHLIGQSWDGGVFWTFFDSQNNREAARQIKPDLS
                     ESIVIPAKVPDKVDPVNRPSPLKPEEPAPKRNNK"
     gene            4756..>5630
                     /locus_tag="PLU11_10260"
     CDS             4756..>5630
                     /locus_tag="PLU11_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPN59550.1"
                     /translation="MSLITVGTMAFDAIETPFGKTDKIVGGSAVYVAYAASNFVKPVQ
                     QISIIGDDFPEEEMADLRSRGVHLDGVEIIKGKKSFFWSGRYHEDMNSRDTLVTELNV
                     LADFDPKIPDSYQGAEFLMLGNLAPKIQLNVIQELKQRPKLIAMDTMNFWMDIAMPDL
                     KIVLRYIDMLIVNDAEARQLTGMFSLVKAAKSIMAMGPKFLIIKKGEHGALLFHGDDV
                     FYAPALPLEDVFDPTGAGDTFAGGFMGHIAKTGDISFENMKRGIIVGSAMASFCVEKF
                     GPTRLKEITAEDINTR"
BASE COUNT         1446 a         1317 c         1298 g         1469 t
ORIGIN      
        1 ataaaataat cacttatgat gccagcagtt ttgtagcgaa taaggaaaag gcggaagccc
       61 tgtttaatca gtatgtggct aaactcgaat cctgcggcac actgcaggac cgccaggtag
      121 cacagaccaa aaacctgatc accctcagct attctgccag ggtgaataac cgggcgaaag
      181 aagtcagtct gaaattaatg gacctcggtg aagggtatgt gatcacggta aatgtgacgg
      241 ataaatatgc aaaactaaat tgactggtca attaatctga aataaacatt acgatgaaag
      301 ggatactttt tttctttacg atgctctgct gctgttccat ttcccaggca caggattcat
      361 taggcgtgta tgtccgggta aaaccggaag cggtgatttt tgatgattat aaactggagc
      421 atgatcccaa ttacaggaaa gctatggcgc aacgggggaa accgtacgcg tctgccgcgg
      481 tccgtatttc cgaacagatg aacagctttt tgaaagggta tccgcagaaa ttcgatatcg
      541 ggaactatga tgcccggatg gacaccatac aaaagtattt gccgggctat catgcgttta
      601 ttgttgcttc ctggaataaa aaatgcagtg attattcctc catacctgaa aacacacccc
      661 tgatattggt ggagattccg cttgagtaca atgatttttt tccgtttgaa ctccagcctt
      721 atccacgcag attctttgtt ctgctctccc cggcctctgt ggaaaagcag tccgaggtgg
      781 cctataccac ttttaaagca gcatataaaa aacgcaaaga accagtcgtc gtaaaaaagg
      841 aaccgactcc gccacctgtg ataaaaacaa ctgaaccggt tgtagtggca cctgcccctg
      901 tggttaagcc tgcgccgcca aagccggcag caacggttga ttacaaccca cccaccggca
      961 ctgcttttaa agcggatggg aaaaatgaag gctatttcca gcataaacgg aaaatggatc
     1021 gattttatgt aaccaacatc agaacagcac tggtcaatgt taattttagt aaccaggagt
     1081 acttcaagag taaactggta ggggttgccg ggtattcgta tatcgacatg tccaccttca
     1141 gttacatgaa tgaatccggg ttttatccgc caggtatccg gaccccggca ttacgcgaag
     1201 cgaatggaaa actggcggct aattacaatt gttatatggt ggagaatctg gtggacaata
     1261 acaataccat gtatgacctg ctttgggtgc cctacagcga aaatctcggg atgccaaaag
     1321 atatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgaa ggcgaaagtg
     1441 tatgccacgc agccttacct ccggtacgat tttgtggaaa tggaacgatg catggatgtt
     1501 cgtaaacatg taaccaaaaa aatcggcagt gatgcggccc gtaacggctc ctgttctttc
     1561 ttttaatctt taataatcat tcaaatgaaa aaagtactct ttctcttttg cctgatctgc
     1621 ctttctgttt ccatgcatgc ccagcagcct gttttacagg ccccctccct gaaccggatg
     1681 ctcgatctgc tgacccgcga ttcagtctcg tatttccggg gtgaagaaaa atcggaccct
     1741 atgaacggga cctatcatgt atccacgcta ccggccatgg gaaaatatgt catcaatatt
     1801 tccgaaagcc ctcagcaacc gatccccttt tcatttttta ttgaagtacc cgtttccaaa
     1861 gcggtcactt ttgccaaaat aaaggcagaa ttagtcgccc tcaccaaagc aaaaaaactg
     1921 aagtataaaa tggtggtgaa ggaaaattat gacgaaagaa tactggcgct gggggatggt
     1981 gtacttatca ttcagattca tgacagcatg ggagagggtg gtaatatccg gattgccgtt
     2041 ttccgcgacg aaaaatatga atagggaagt gctgccgcac cggcagctta tttcgcatca
     2101 aagtccacca ctactttttc cgttttcggg tgactctggc aggtgaggat ataacctttg
     2161 gctacttcct cctcttccag tccccaatgt acatccatgc tcacttcgcc ttcaagcagt
     2221 ttggctttac aggtacaaca catgccaccc ttacaggcat aaggcaggtc cgccccctgc
     2281 tgcaacgccg catcaagaat ggtctgatca ctgttaaaag gaatggtcac ctcaaagctg
     2341 cgcccatcca cctttacatc aatattgctt acggccccgg ctgtattgat ctgtggactg
     2401 tcgactgtgg actgtcgact ctttgtgctg ttaaacaact ccaggtgaat cttatttccc
     2461 gcaatccctt tcttttccag aaaagctttt accgtaaaga tcatctctgt cggtccgcag
     2521 atgaaaaatt catcggtgcc ggtaaagtct tctaaacggt ccagctcttc cagcttctcc
     2581 tggctgatcc gtccgaaatt aagatcggta tccatccgtt cccggctcag gatatggacc
     2641 aggctgaaac gctccatata ctggttcttg agtgcttcca gttcttcaaa gaaaataatg
     2701 gagttccggt tccggttccc gaataccagg gtaaagcggc tatgcttctc cgtggccagg
     2761 gtggttttaa tcagggagat caccggggtg atgccactgc cggcggcgaa agcgagataa
     2821 ttctttttat tttcaggatg aagcggggtg ttgaatttgc caacaggcgg cattacatcc
     2881 agcacatccc cctttttcaa tagctcattc gcaaaagaag aaaacaggcc accttccact
     2941 tttttgatcg ccactttgag tttattctcc agcggcgagg aacatataga gtagttacgg
     3001 cggatttctt cgccattgat cgtggtgcgg agggtcagac tctggccctg gctgaactga
     3061 aaatcgtttt gcagggtttc aggtacatca aaaagtacag aaacacattc aggggtttct
     3121 ttctttacct cttttaccgt gaggcggtgg aaatgaaggg acatatcagc cttgtttacg
     3181 taatccgtcg atcatgatca taaccatatt attctcgagt tcatcggcac tgatcttggc
     3241 cgtactcctg tgccagcttt caataccgct gaccgcatgg agcatgatga ggaccgctgt
     3301 cggggcatct attttgcgga tctcattttt cgcaatgcct tcttctatga tggaagcaaa
     3361 ttttttacgg taattacggc gttgggtctg gaaattagag agataaggct catccaggtg
     3421 tttccactcc cggtcactca catacacatc ctcatagttt tcggtcatct gccggatgtg
     3481 aaaacgtaag agttgatcaa ttttagcaat cgacttctgg ttactggcct caatcgtatc
     3541 gatcgtggta ttatacacat tggctacgtc aaagcagatc gcttccagta tctcgttttt
     3601 agaacggata tggttataca ggctggccgc ttcaatacct accgcttctg cgaggtcacg
     3661 catggaagtg gcggcgaatc ctttttcgcg gaacattgcg gctgcttttt tcaggatcag
     3721 tccttttttg gacgcttttt tacgttgggc ctttgccata gtcattaatt ataagtaaca
     3781 agtagaaata taaatatagg gaactattct catttgttat ttcgtttggg agccggttct
     3841 tccggtttca acggggatgg acggttaacc ggatccactt tgtcggggac ttttgccggt
     3901 atcacgatgg actcgctcag gtcgggtttg atctgccggg ccgcctcccg attattctga
     3961 gaatcaaaaa aggtccagaa aacaccgcca tcccaggact ggccgatcag gtgacttttc
     4021 gaggtgattc tcttaccatc ggtctcgatg atggaattca tttcaatcat gcattgcaga
     4081 tcggcgccgc ttttgatgac ctgtaaaacc aaaccgggtt tatacaattt ccaggcatca
     4141 ggggagatat ccccgaacat ggatgcgatc gtccggataa actcttcttt cccgcccagg
     4201 ctaccaatca ttgccgggtt gctataccgg gcaaatacct cccagttccg gttacggaat
     4261 ccataggcca ggctgtcggc gcatttggtg atctggctct ttacaaaact gctgtcaaac
     4321 tgggcattgg catccagggt cactaaaagg accaggatca gcagcagccg gcagaaaaag
     4381 cggttttgtt tcatagtatc aaaggtatag aatcggaagg gactaagtat cccggtctta
     4441 cgggtagcag gcattaagta cgaggtacga ggtacgaccc ttcgatacgt cccgcatgaa
     4501 aatttttcac aagcctgaaa aactagtttt gcgggactac tcccaccttc acttcgtttc
     4561 ggtcggcagg cagggtgaca agaagatcag taaaacgagg aacatcaggt tattttagat
     4621 taaaacttct caggaggtcc tgacacccga cacctgacac ccgacacctg acacccgaca
     4681 cctgatacct cccaactatc gtctcttttc agtagttttg cacgccttta cgtaataaaa
     4741 tacaaaaaac aaatcatgtc tttaatcacc gttggaacca tggcttttga tgccatcgaa
     4801 acaccctttg gaaaaaccga taaaatcgtc ggaggatctg ctgtttatgt ggcttatgcc
     4861 gccagtaatt ttgtgaaacc ggtacagcag atttcgatta tcggggatga ttttcccgaa
     4921 gaggaaatgg ccgatttacg cagccgtggg gtgcacctgg acggggtgga gattattaaa
     4981 ggcaagaaat cctttttctg gagcggtcgt tatcacgaag acatgaacag ccgcgataca
     5041 ctcgtgactg aactgaacgt actggctgac tttgatccga agatcccgga tagctaccag
     5101 ggagccgaat tcctgatgct gggtaatctg gcacccaaga tccagctgaa tgtgatccag
     5161 gagctgaaac aacgtcccaa gctgattgcc atggatacca tgaacttctg gatggatatt
     5221 gccatgcccg acctgaagat cgtacttcgt tatattgata tgctgatcgt gaatgatgcc
     5281 gaagcccgcc agctgaccgg catgttctcc ctcgtaaaag cggccaaatc cattatggcc
     5341 atggggccga aattcctgat cattaagaaa ggagagcacg gcgctttatt attccacggc
     5401 gatgatgtgt tctatgcacc cgccctgcct ttggaagatg tgttcgatcc gactggtgcc
     5461 ggtgatacgt ttgccggtgg tttcatggga catatcgcca agaccggcga tatctccttt
     5521 gaaaacatga agcgcggtat catcgtgggt tcggccatgg ccagcttctg cgtggaaaaa
     5581 tttggtccta cccggctgaa ggaaatcacc gctgaagata tcaatacccg
//
LOCUS       DAOPMW010000212         5620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16690_length_5620_cov_6.508176, whole genome shotgun sequence.
ACCESSION   DAOPMW010000212 DAOPMW010000000
VERSION     DAOPMW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16690_length_5620_cov_6.508176"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2203)
                     /locus_tag="PLU11_10265"
     CDS             complement(<1..2203)
                     /locus_tag="PLU11_10265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPN59551.1"
                     /translation="MRLLPLATSVVITAGLVFALNKQWGAVPPMGKFLNPQYGFWQNA
                     EPVGADYDASISLPGLKGKADVYLDDRLVPHVFAENDEDLYFIQGFLHAKFRLFQMDL
                     QTKAAAGRASEIAGPKAIAFDREQRRLGMVYAAENALKEIEKDPDSKKQYDAYTNGVN
                     AYLADLKEADLPVEYKLLNIVPEKWSNFRTALLLKMMAKMLSSGTESDLANTNAKSIF
                     IPDELKLIYPQVPDSLVPIVPKGTPFAAPGIVPIAPVSADSLYIGRKDTVTAKEISKP
                     DINNGSNNWVVAGSKTASGSPILCNDPHLELSLPSIWYEIQLQTPTSNAYGVSLPGSP
                     FVIIGFNEHIAWGVTNAQRDVKDYYEIRFRDESKQEYFFNGKWEKTTLKEETIKIKGQ
                     ADLKDTVAYTIFGPVMFDDKFSNELSNNRNLAVRWVAHDPSNEGYALYKLNRATNYDD
                     YAEAIKGFQCPGQNFIFASKTGDIAIWQQGKFPARWDRQGLFVMPGEDSSYMWQGFIP
                     QPENPHAKNPERGFLESANQRAADSTYPYFIPGSYLTPRGITIENKLAAMSSITPKDM
                     MVLHSNYFNTLAEDARGIMLQYVKENELDPVAKHFLGIFREWDLNASPDSKGQTIYQC
                     WWDSLEVEIWKDELSRSTQLVPWPEEQITMELLKKDSALKYIDNINTAEKETLFDVVT
                     TALKKAATDLAKKETEGKLEWTKFKNPTVYHLIKDLKGFARPGLPVGGYGNI"
     gene            2349..2909
                     /locus_tag="PLU11_10270"
     CDS             2349..2909
                     /locus_tag="PLU11_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPN59552.1"
                     /translation="MPVLIVLGIIALIIVWVISLYNGLVRLRNRRQNAFADIDVQLRQ
                     RHDLVPQLVDTVKGYASHEKELLTRITEARTAAMSATSIDGKIVAEQQLSAALSGLRV
                     QVEAYPDLKANQNFLQLQEELSDIENKLAAARRFFNGATTEYNNAVESFPGNLVARNF
                     GFAKEIMFDLGTDTRKQMEEPPKIQF"
     gene            2936..3874
                     /locus_tag="PLU11_10275"
     CDS             2936..3874
                     /locus_tag="PLU11_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPN59553.1"
                     /translation="MEYVGLDKQIRRNNFNSTLLLIAFPALLLGMLYALVYFTREDHG
                     EDPNQLFINTAPLVLAGVGIWFLIAWAGHSTFIRLATGSKHLERTENKRVYNLLENLC
                     ISQGMTMPKLYIIEDDSLNAFASGIDQRSFSISLSRGIINKLDDEELEGVIAHELSHI
                     KNRDVRVLIITIIFVGIFSFLAELAFRSLRFTGGGRKSSKDSKGSGAIILIAIVVTAV
                     AYFISLLLRFGISRRREYLADAGAADMTKKPYALANALRKVSNDPAIEAVESRDVAQL
                     FFDNPKPSSHKSASWDNMFSTHPPIDKRIELLEQFV"
     gene            complement(3932..4408)
                     /locus_tag="PLU11_10280"
     CDS             complement(3932..4408)
                     /locus_tag="PLU11_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59554.1"
                     /translation="MNKSKTAFISTIVTIVAVFSLIWMLDRLKDQDRTPVIIHAFRSG
                     LEPEMFSIDFRGNGTYKCGKGLFLGNHHYMRGRYTIKDSILILDRSNLYNQIISNRLL
                     LKTIPQADSLKRKQGFLGKLLGLPKPDTTAKTYLFQLDHQSDTIPSALVLKVKWIK"
     gene            complement(4810..5340)
                     /locus_tag="PLU11_10285"
     CDS             complement(4810..5340)
                     /locus_tag="PLU11_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59555.1"
                     /translation="MGKIDDEPAEVLPGCSWYCGGNVTGFKVSSSLAPAGKISFSANK
                     AHDFDITTAWVEGKPDNGIGEYLEYAIDMTADNASQKLGITQIILANGYKKSKATWQE
                     NARVKKLKMVVNGKPYGMLELLDAIEFQVIDIGKIMLPPQKITTIRFEIVEVFPGSKY
                     KDTAISELLFDGVGVH"
BASE COUNT         1471 a         1310 c         1246 g         1593 t
ORIGIN      
        1 cgatattacc ataacctcct accggtaatc ccggccttgc aaatcccttg aggtctttga
       61 tcaggtgata gacggtgggg tttttgaatt tagtccattc cagtttaccc tccgtttctt
      121 ttttagccag gtcagttgcg gctttcttca gggcagtggt caccacatca aaaagggttt
      181 ctttttctgc cgtattgata ttatcgatat actttaaagc cgaatccttt ttcagcagtt
      241 ccatcgtgat ctgttcttcg ggccagggca ccagttgggt actgcgggat aattcatcct
      301 tccaaatctc cacttccagg ctatcccacc agcattgata gatggtttgt ccttttgaat
      361 caggggaggc attcaggtcc cattcacgga aaatacccag gaagtgtttc gctacgggat
      421 ccagttcatt ttcttttaca tattgcagca tgattccccg tgcatcttcg gccagggtat
      481 taaagtaatt gctgtgcagc accatcatat ccttgggagt aatgctgctc atggcagcca
      541 gcttattctc aatggtgatt ccccggggcg tcagatagct gcccggtata aaatatggat
      601 aagtggaatc tgcggcacgc tggttagcac tttcgaggaa tccacgctcc ggatttttgg
      661 catgcggatt ttccggttgc ggtataaagc cctgccacat ataactgctg tcttcaccgg
      721 gcatcacaaa caatccctgg cgatcccagc gggcgggaaa tttaccctgc tgccagatag
      781 cgatatcgcc ggttttggaa gcaaagatga aattctgtcc cggacattga aatcccttaa
      841 tggcttccgc atagtcatca tagttagtcg cccggttcag tttgtataaa gcataacctt
      901 cattactcgg atcatgggcc acccagcgta cggccaggtt gcggttattg gacaactcat
      961 tagagaattt atcatcaaac atcacggggc cgaagatggt ataagccaca gtgtctttca
     1021 gatcagcctg ccctttaatt tttatcgttt cttctttcag cgttgttttt tcccatttcc
     1081 cgttaaagaa atattcctgt ttgctttcat cgcggaaccg gatctcataa tagtccttta
     1141 catcccgttg ggcattggtt actccccagg caatgtgttc attgaacccg ataatcacga
     1201 agggacttcc cggcagggac accccgtaag cattgctggt tggtgtctgt aattgtattt
     1261 cataccagat cgaaggcagg gaaagttcca ggtgcgggtc attacaaaga atgggtgaac
     1321 cactggcggt tttgctacca gccactaccc agttattact gccattattg atatccggtt
     1381 tggagatttc ttttgcggtt acggtatcct tcctgccgat atacagggaa tctgcactga
     1441 ccggcgcaat gggcacaatt cccggggcag caaagggcgt gcctttcggt acgatcggca
     1501 ccagggaatc gggcacctgc gggtaaatca gttttaattc atccggaata aaaatggact
     1561 tggcattggt attggccagg tcactttctg taccggagga cagcattttc gccatcatct
     1621 ttaataagag tgccgtacga aagttgctcc atttttcagg cacgatattg agcagtttat
     1681 actccaccgg caggtcggct tctttcaggt cagccaggta agcattcacg ccattggtat
     1741 acgcatcata ttgttttttt gaatcgggat ctttttcaat ttctttcagg gcattttccg
     1801 cagcatagac cattcccagc cgacgttgct cccggtcaaa ggcaatggcc ttgggtccgg
     1861 cgatttcact cgcacgaccg gctgcggcct tggtctggag gtccatctgg aagagccgga
     1921 atttggcgtg caggaaaccc tggataaaat acaggtcttc gtcgttttcg gcaaacacgt
     1981 ggggaacgag gcggtcatcg aggtagacat ccgcttttcc tttgagtccg ggcagactga
     2041 tggacgcatc ataatccgct ccaactggtt cggcattctg ccagaagcca tattggggat
     2101 taaggaattt tcccatcggg ggcacggctc cccattgttt attcagggca aaaacaagac
     2161 ccgctgtaat gacaacggaa gtagctaagg gcaataatct catgaagaag caattaggag
     2221 cgaaatatac aactttattc ggcttttaaa aacgggattc cactgataaa aactaccgta
     2281 gtgtccccaa aacagctttt agtggtattc ttattttttg cgtaatttca ggaactaaac
     2341 acactattat gccagtctta attgtccttg gaattatagc cctcatcatc gtttgggtca
     2401 tcagtctcta caacggactc gtgcgtctcc gcaaccgtcg tcagaatgct tttgctgata
     2461 ttgatgtaca gcttcgtcag cgacacgacc tggtacccca gttagtggat acggtaaaag
     2521 gctatgccag tcatgaaaaa gagttattga cccggatcac agaagcccgt acggccgcca
     2581 tgagtgccac cagcattgat ggaaagattg tggccgagca acaattaagt gcagccctgt
     2641 ccggattacg tgtacaggtg gaagcctatc ctgatctcaa agccaaccag aatttcctgc
     2701 agttacagga agagctgagt gatattgaaa acaagctggc agccgcccgt cgtttcttca
     2761 atggagcaac aacggagtac aacaatgcag tggaatcctt ccccggcaat ctcgtggccc
     2821 gcaatttcgg atttgccaaa gagattatgt ttgacctggg taccgatacc cgcaagcaga
     2881 tggaagagcc gccgaaaatt cagttttaat acagggttga tcccactaac tgcaaatgga
     2941 atacgtaggc ttagacaaac agatccgccg gaataatttc aattccacgc tcctgctgat
     3001 tgcttttccg gctttattgc tggggatgct gtatgcattg gtttatttta cccgcgagga
     3061 tcatggggaa gaccccaatc aattatttat aaataccgcg cccctggtat tagccggtgt
     3121 aggcatctgg ttcctgattg cctgggccgg gcattccact tttatccggt tggctacggg
     3181 cagcaagcat ttggaacgga ccgagaataa aagggtttac aacctgctgg agaatctctg
     3241 tatttcccag ggcatgacca tgcctaaatt atatatcatc gaagacgact ctctgaatgc
     3301 ctttgccagc gggatcgacc agcggtcttt ttccatttcc ctctcccgcg gcatcatcaa
     3361 caaactggat gatgaggaat tggaaggcgt gatcgcccat gagttgtccc atatcaaaaa
     3421 ccgggatgtg cgggtgctga tcatcactat catttttgtt ggcatcttct ctttcctggc
     3481 cgaactcgct ttccgcagtc tccgttttac cggcggtggc cgcaagagca gtaaggactc
     3541 caagggcagc ggcgccatca tcctgattgc gattgtagtg actgctgtag cctatttcat
     3601 ctccctcctc ttacgttttg gcatcagtcg ccgccgcgaa tacctagccg atgccggagc
     3661 ggcagacatg accaagaagc cctatgcgct ggccaatgct ttacgtaaag tatctaatga
     3721 tcccgctatc gaagcagtcg agagccggga tgtggcccaa ttgttttttg ataatccgaa
     3781 gccatcttcg cataaatcag cttcctggga taatatgttt tcgacacatc ctcccattga
     3841 taagagaatt gagttgctgg agcagttcgt gtgagtcagt tgtcagatgt cagatgtcag
     3901 ttgtcagggc ctgctaagag acttgggttt attacttgat ccatttcacc ttaaggacaa
     3961 gagcggaagg aattgtatcg ctctgatggt cgagttgaaa taagtaagtt tttgctgtag
     4021 tatccggttt gggtaggcca agcagttttc cgaggaatcc ctgctttctt tttaatgaat
     4081 cagcttgggg aatggttttc agcaaaagac ggttactgat gatctggttg tacagatttg
     4141 atctgtccag tatcaaaata ctatccttaa tcgtatagcg accacgcata taatgatgat
     4201 ttcccaaaaa aagtcctttc ccgcatttat aagtaccatt tcccctgaaa tcaatagaga
     4261 acatttccgg ctccaatcca ctcctgaaag catgtataat tacaggtgta cggtcctgat
     4321 cttttaagcg atcaagcatc cagattaagg aaaaaacagc tacaatcgtc acgattgtgg
     4381 aaatgaatgc cgttttggat ttattcaact tatacctatt aatatccttg taaatacata
     4441 tcagggaaac aatggaagcc aagatcagga atccagcatg aacaaaactt tccaggagat
     4501 attgatggtc gaagtcgcta taaaaccgat agatgcataa aatccatacg acaagaatta
     4561 gtactattct gccaataaaa atctcgcccc ttctcattcg taatttaatt cgttttagat
     4621 ttccctatcg tatataaata catactataa acgccagaaa acactactct ctatttcatc
     4681 atgaattcgc ccgacaataa atatctacat atatccacaa aattgaataa gacaaattta
     4741 taaaccaatt ttatttgcaa agttgtgggc tctatgggat caggtcatgc atatctgaaa
     4801 gtcgggtatt taatgaacac caacaccgtc gaaaagtaat tcgctgatag ctgtgtcctt
     4861 gtatttagaa cctgggaaaa cttcaacaat ttcgaatcgg atcgttgtga ttttctgagg
     4921 cggcagcatg attttgccga tatcaatgac ctgaaattcg atagcatcca gtaattccag
     4981 cattccatag ggcttaccat tcacgaccat tttcagcttt tttacccggg cattctcctg
     5041 ccaggttgct ttcgatttct tatacccgtt cgcaagaatg atctgtgtaa tacccagttt
     5101 ctgactggca ttatcggcag tcatatcaat cgcgtattcc aagtactctc cgatcccgtt
     5161 atctggtttt ccttccaccc aggctgttgt tatatcaaaa tcgtgggcct tatttgccga
     5221 aaaagaaatt ttgcctgcgg gtgccagaga ggaggatact ttaaatccgg ttacatttcc
     5281 accgcaatac cagctgcatc ctggtaaaac ttcagcaggc tcatcgtcaa ttttacccat
     5341 aaaatcagca tccttagcct gtacagcttt gaataccgga atttggtgcg gaacagaggc
     5401 tgaaaaagaa aataaataga ttgtacaaaa aagaatcttc atgatttctt aaacaaatta
     5461 aaatttatac tatcaaaacg ttgacaaata aatctaatct ttagttaatg tagaaaggat
     5521 agtaattacc gagtcaagcg acgatcgatg gagggctgaa gctgtaaaac tgaaccctcc
     5581 tattttcata aaactggtat aataacccgt tcccgtttgg
//
LOCUS       DAOPMW010000213         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16708_length_5615_cov_8.510252, whole genome shotgun sequence.
ACCESSION   DAOPMW010000213 DAOPMW010000000
VERSION     DAOPMW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16708_length_5615_cov_8.510252"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1133
                     /gene="gmd"
                     /locus_tag="PLU11_10290"
     CDS             21..1133
                     /gene="gmd"
                     /locus_tag="PLU11_10290"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN59556.1"
                     /translation="MNKTALITGITGQDGAYLAEFLLKKGYIVHGIKRRSSLFNTDRI
                     DSLYEDPHISNRHFVLHYGDLTDSTNLIRIIQQVQPDEIYNLGAMSHVKVSFDVPEYT
                     ANADGIGTLRILEAVRLLGLTKKTRIYQASTSELYGLVQEVPQSETTPFYPRSPYGVA
                     KMYAYWITVNYREAYDMFACNGILFNHESPLRGETFVTRKITRAVAQMSLGMQEKLYM
                     GNLDAKRDWGHAKDYVEAMWLILQQEKPEDFVIATGITTPVRDFIRMAFREVGVELGF
                     KGEGSEEKGYVASSNDAVHFPVGKEVVAVDPNYYRPTEVDLLIGDPTKANTVLGWKPK
                     YTLDAMVKEMVAADIALFQKNKILKEAGFNVLRQFE"
     gene            1278..2210
                     /locus_tag="PLU11_10295"
     CDS             1278..2210
                     /locus_tag="PLU11_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPN59557.1"
                     /translation="MEKNSKIYIAGHRGMVGSALMRKWQAEGFTNIITRTSAELDLRN
                     QAAVTDFFSAAKPEYVILAAAKVGGIVANNTYRAEFLYENLMIQNNVIHQSYVNGVKK
                     LLFLGSSCIYPKLAPQPLKEEYLLTGSLEHTNEPYAIAKIAGLKMCEAYHDQYGCNFI
                     SVMPTNLYGPNDNYDLKNSHVLPALLRKFHEAKESGAPSVNLWGTGSPLREFLHSDDL
                     AAACYFLMRDYNETKPVNIGVGHDISIRDLAGLIQRITGFTGTIEWDSSKPDGTPRKL
                     MDVSRINAAGWKASITLEEGITRVYQETFLTGKP"
     gene            2213..3040
                     /locus_tag="PLU11_10300"
     CDS             2213..3040
                     /locus_tag="PLU11_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN59558.1"
                     /translation="MAYKIIIESGRAEKNYWKDLWHYRELFYILSWRDIKVRYKQTTL
                     GVLWAIIRPLLTMIIFTFVFGKVAKMEVQSVVPYALIVFAGLLPWQFFSNALTESSNS
                     LVGNERLVTKVYFPRMIIPASSVITSFIDFLISFAILLVLFVVYQYMPSARIFWMPLF
                     WIMAFLASFGPGLYLTAMNVKYRDFRYIIPFIVQFGLFISPVGYTSAQIPEKYQAIYA
                     LNPMVGVIDGFRWCIVKDAPNPMLHYPFYISLAVIVFFLALAVYQFRKMEKNFADLI"
     gene            3065..4000
                     /locus_tag="PLU11_10305"
     CDS             3065..4000
                     /locus_tag="PLU11_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN59559.1"
                     /translation="MNTILVTGGAGNVGGSLAKKLVTNPDNFVVVMDDLSTGNAGKLP
                     DATRYPNFKFIKGDVNSLSDISAVILRYGFDFIFHYAAVVGVKRTLDNPAKVLHDIQG
                     IKNVLEVAKNTGVKRVFFSSSSEVYGEPVEFPQNEHTTPLNSKLPYAIVKNVGEAFLK
                     SYKQEYDLDYTIFRFFNTYGPDQSSDFVISKFIDLALVNKPITLYGDGLQTRTFCYID
                     DNIEATTNALYENKFVNDVVNIGSDYEMTIKELAELIIKEARSSSPIIHLPPLPEGDM
                     TRRKPDIGKMKQLLSREMTPVTEGVRRILALRKLS"
     gene            4029..5225
                     /locus_tag="PLU11_10310"
     CDS             4029..5225
                     /locus_tag="PLU11_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59560.1"
                     /translation="MSEPIIKVEGLGKKYIISHEQRESYTALRDVISRKAKSLTSRGK
                     IKTVREDFWALKDVNFEINPGEAVGIIGRNGAGKSTLLKVLSRITEPSTGRIEINGRI
                     ASLLEVGTGFHPELTGRENVFLNGAILGMTRSEIRSKFDEIVAFSGVEKFLDTPVKRY
                     SSGMYVRLAFAVAAHLEPEILVVDEVLAVGDAEFQNKCLGKMNEVSHQEGRTVLFVSH
                     NMRAIQDLCQKSIYLQNGQVLDIGPTDQVIEHYLNTGTSQTWSDATGPVQLVEVNQDK
                     KEIKIDVQYKVSDEYVLEIPNFGFVIYDQMNTPVCGFNPSLLKEPLPLKQYRKEGRIQ
                     ITMREPRLLDGRYNFSFWFGVNSQFDIQYVKDAISFEVVGMTEHKQLPSNILGTSGVE
                     CQFDFA"
     gene            5257..>5615
                     /locus_tag="PLU11_10315"
     CDS             5257..>5615
                     /locus_tag="PLU11_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59561.1"
                     /translation="MKFTVTANKQFPEIKEYAVKNAAIGFYGYVFKGLQNLQPIMPAD
                     YRPLSFYSVLRNTVSRKTVRVDKPALSIVNSWYDNFYHFAWESLVKLYFLRDHIDTAT
                     VVFPEERRKFHDQWLNLL"
BASE COUNT         1480 a         1369 c         1383 g         1383 t
ORIGIN      
        1 ccattcaacc tcttcaaact atgaataaaa cagcactcat caccggtatc accggtcagg
       61 acggcgccta cctggcggaa tttctcctta aaaaagggta catcgtacat ggaataaaaa
      121 gaagatcctc cctgttcaat acagaccgga tcgatagcct gtatgaagat ccccatattt
      181 ccaaccggca ttttgttttg cattatggcg atctgaccga cagcacgaac ctgatccgca
      241 ttatccagca ggttcaaccg gacgagatct ataacctcgg ggccatgagc catgtaaaag
      301 tgagttttga tgtgccggaa tatacggcca atgccgacgg gatcggtacc ttacgcatac
      361 tcgaagccgt tagattgctc gggctgacca aaaagacccg gatctaccag gcctccacgt
      421 ccgagttata cggattggtt caggaagtgc cgcaaagtga aaccacgccg ttctatcccc
      481 gcagtccgta tggggtggct aaaatgtatg cttactggat cacggtcaac taccgtgaag
      541 cctacgatat gtttgcctgt aacggtattc tttttaatca cgagagtcct ttacggggcg
      601 aaacctttgt aacccgcaaa atcacccgtg ccgtggccca gatgtcactc ggtatgcagg
      661 aaaaattata catgggtaac ctcgatgcca agcgtgactg gggtcatgcc aaggattatg
      721 tggaagccat gtggctgatc ctgcagcagg aaaaaccgga ggattttgtg atcgctaccg
      781 gcatcaccac cccggtccgt gattttatcc ggatggcttt ccgtgaagta ggcgtggagc
      841 tcggattcaa aggagaaggc agtgaagaga aaggatatgt agccagctcc aacgacgcgg
      901 tacatttccc cgttggtaaa gaagtagtag ccgtggatcc gaactattac cgtcctaccg
      961 aagtagacct gctgatcggt gaccccacca aggccaatac ggtactgggc tggaaaccca
     1021 aatacaccct cgacgccatg gtgaaggaaa tggtagccgc cgatatcgcc ctgtttcaga
     1081 aaaataaaat tttaaaagaa gccgggttta atgtactgag gcagtttgaa tagtctctcg
     1141 gtatggttcg gcacccttcg gcatggttcg gcaagcctgc ctgtccggta ggcaggctca
     1201 gggtgacaag tgctgcttgt tgtcatggtg agcgtgtcga accatgacta gcaaaaacat
     1261 caattcaaga gaccacaatg gaaaaaaaca gtaaaatcta cattgccgga caccgcggaa
     1321 tggtgggcag tgcgctgatg cgtaaatggc aggccgaagg ctttaccaat attatcaccc
     1381 gcacttctgc ggaactagac ctgcgtaacc aggcggcagt aaccgatttc ttttcggccg
     1441 caaagccgga gtacgtgatc ctggcggcgg ctaaagtggg tggtattgta gccaataata
     1501 cctaccgcgc cgaatttctt tacgaaaacc tgatgatcca gaacaatgtg attcatcagt
     1561 cctatgtaaa cggcgtgaag aaactcctgt ttctcggctc ctcctgtatc taccccaaac
     1621 tggccccgca accattgaag gaagaatacc tgctgaccgg ttcactggaa cataccaatg
     1681 agccctatgc tattgccaag atcgcgggac tgaaaatgtg cgaagcctat cacgatcagt
     1741 acggatgcaa tttcatttcg gtgatgccca ccaatctgta tggcccgaat gataattatg
     1801 acctgaagaa ttcccatgtg ctgccggcgc tcttacgaaa attccatgaa gccaaagaat
     1861 ccggcgcacc cagcgtaaat ctatggggta ccggttcacc cctgcgggaa ttcctgcact
     1921 cagatgacct ggccgctgcc tgttatttcc tgatgcggga ttataatgaa accaaacccg
     1981 tgaatatcgg ggtgggccat gatatcagta tccgcgatct cgcgggactt attcagcgga
     2041 tcaccggatt taccggaacg attgaatggg acagcagcaa acccgatggc actccccgca
     2101 aactgatgga tgtaagccgc atcaatgcag ctggctggaa agcatccatc accctggaag
     2161 aaggcattac ccgcgtatac caggaaacat ttttaaccgg taaaccctaa gcatggcgta
     2221 taaaattatt attgagtctg gtcgtgcgga aaaaaattac tggaaagacc tctggcatta
     2281 ccgggaactg ttttatatcc tgagctggag ggatattaaa gtacgctaca agcaaaccac
     2341 gcttggtgtg ctctgggcca tcatccgtcc gttgctcacc atgatcatct ttacttttgt
     2401 atttggcaaa gtggccaaaa tggaagtaca gagtgtggtg ccttatgccc ttatcgtttt
     2461 tgccggactg ctgccctggc agtttttctc caatgctttg acagaaagca gcaacagcct
     2521 tgtgggcaat gagcgcctgg tgaccaaagt ctattttccc cggatgatta taccggccag
     2581 ttcggtgatc accagcttta tcgacttcct gatttctttc gcgatcctgc tggtattatt
     2641 tgttgtgtac cagtatatgc cttcagcccg tattttctgg atgcccctgt tctggatcat
     2701 ggctttcctg gcttcttttg gtccgggact ttacctcacc gcaatgaatg tgaagtaccg
     2761 cgatttccgt tatatcattc cgttcattgt acagtttggt ttatttatct ctcctgttgg
     2821 gtataccagt gcgcagatcc cggaaaaata ccaggccatc tatgccctga accccatggt
     2881 gggtgtgatt gatggtttcc gctggtgcat tgtaaaagat gccccgaatc ccatgctgca
     2941 ctatccattc tatatttcac tggcagtgat cgtttttttc ctggccctcg cggtgtacca
     3001 gttcaggaaa atggaaaaga actttgccga tcttatttaa tagcattaaa actaatcccc
     3061 aagaatgaat accattttag taacaggagg cgccggtaat gttggcggtt ccctggccaa
     3121 aaaactggtc accaacccgg ataattttgt agtggtcatg gatgacctct ccaccggcaa
     3181 tgccggtaaa ttacccgatg ccacccgcta tccgaacttt aaatttatca aaggggatgt
     3241 taactccctg tcggatatct ccgcggtcat tctgcgttac gggtttgatt tcatctttca
     3301 ttacgcggcc gtggtagggg tgaagcgcac cctcgataac ccggccaaag tattacacga
     3361 tatccagggt attaaaaatg tactggaagt agccaagaac accggcgtga aaagggtgtt
     3421 cttctcctct tcttctgaag tatacggtga gccggtggaa tttccgcaga atgaacatac
     3481 caccccgctc aattccaaac tgccctacgc cattgtaaag aatgtgggcg aggccttcct
     3541 gaaatcctac aaacaggaat acgatctgga ttataccatc ttccgtttct ttaataccta
     3601 cggaccggat cagagcagcg attttgtgat ctccaaattc attgacctgg ccctggtgaa
     3661 taaaccgatt acattgtacg gggacggatt acaaacacgg accttctgct atattgacga
     3721 caatatcgaa gccaccacca atgcgctgta tgaaaataaa tttgtgaatg atgtggtgaa
     3781 catcggttcc gattatgaaa tgacgatcaa ggaactggcg gaactgatta ttaaagaagc
     3841 ccgttcttcc tctcccatca ttcacctgcc gccattgccg gaaggggata tgacccgccg
     3901 caagccggat atcggcaaga tgaaacaact tctttcgcgg gaaatgaccc cggtgacaga
     3961 aggggtacga agaatactgg ccctgagaaa attatcctaa ctgaacagca taccaaagac
     4021 cggagcaaat gagtgaaccc attatcaagg tagaaggact cggcaagaag tatatcattt
     4081 cgcatgaaca acgcgaaagc tataccgccc tgcgcgatgt gatcagccgg aaagcaaaaa
     4141 gcctgaccag tcgcggcaaa atcaaaacgg tgcgggagga tttctgggcc ttaaaagacg
     4201 tgaacttcga aatcaatccc ggtgaagcgg tagggattat cggccgcaac ggcgcgggaa
     4261 agagtaccct gctcaaagta ctgagccgga tcactgaacc cagcaccggc cggattgaga
     4321 tcaatggacg gatcgcctct ctgctggaag taggcaccgg ttttcacccg gagcttaccg
     4381 gccgcgagaa tgtatttctg aacggtgcga tcctggggat gacccggtca gagatccgga
     4441 gtaagtttga tgagatcgtg gccttcagtg gcgtggaaaa attcctcgat acacccgtga
     4501 agcggtacag cagcgggatg tatgtgcgac tcgcctttgc cgtagcggcc cacctggaac
     4561 cggagatact ggtagtggat gaagtactgg ccgtgggtga tgccgagttt cagaataaat
     4621 gtctcggtaa aatgaatgaa gtgagtcacc aggaaggccg gacagtttta tttgtgagtc
     4681 ataatatgcg ggccatccag gatctctgtc agaaatccat ttacctgcag aacgggcagg
     4741 tactggatat tggtccaacc gaccaggtga tcgaacacta tctcaatacc ggtacttcac
     4801 aaacctggtc ggatgccact ggtccggtgc agctggtaga agtaaaccag gataaaaagg
     4861 aaataaagat tgatgtgcag tataaagtga gcgatgaata tgtgctggag attcccaact
     4921 tcgggtttgt gatctatgat cagatgaaca cccccgtttg tggctttaat ccttccttgc
     4981 tgaaggagcc tcttccattg aagcaatacc ggaaagaggg ccgcattcag attaccatgc
     5041 gtgaaccccg tctcctcgat ggccggtata atttcagttt ctggtttggg gtgaattcac
     5101 agttcgatat acagtatgtg aaagatgcca tctcctttga agtggtgggt atgaccgaac
     5161 acaaacaatt accctccaat atcctgggca caagcggggt ggaatgtcag tttgattttg
     5221 cctgagcccg ctgttacagt aacccttttt gatagtatga aattcaccgt taccgccaat
     5281 aagcagtttc ccgaaataaa agagtatgcg gttaaaaatg ccgccatcgg attttatggc
     5341 tatgtattta aaggcttgca gaacctgcag ccaatcatgc ctgcggatta ccggcccctc
     5401 agtttttatt cagtgctccg caatacggtg agccggaaaa cggtccgggt ggataaaccg
     5461 gccttaagca ttgtcaacag ctggtacgat aatttttatc attttgcctg ggagtcgctg
     5521 gtcaaactct acttcctgcg cgatcatatt gataccgcca ccgtggtctt cccggaagaa
     5581 cggcggaaat ttcatgatca gtggctgaac ctgct
//
LOCUS       DAOPMW010000214         5589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16820_length_5589_cov_7.703289, whole genome shotgun sequence.
ACCESSION   DAOPMW010000214 DAOPMW010000000
VERSION     DAOPMW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16820_length_5589_cov_7.703289"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..797)
                     /locus_tag="PLU11_10320"
     CDS             complement(93..797)
                     /locus_tag="PLU11_10320"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HPN59562.1"
                     /translation="MHSLRFLYYTVLLQSGLCLQAQEKDPGIRLALQLDSIASSAAPS
                     SHFAKLYTETVLISLQHYQRADAREKEFIQRFEQTFAGYFFRAAASRGRGHGSEAWQV
                     YFGDSTLTPLQYQLLGINAHINADLSETLTGVFTVKELQSNKKTFIRFQEGLRNQFKQ
                     FYVENVQATRLTRLLDKLTLGLARSYGSIMMSRWRKRQFRLAIWQSTHPEKEARLKRK
                     TTRCKERIDRLILRHL"
     gene            892..2031
                     /locus_tag="PLU11_10325"
     CDS             892..2031
                     /locus_tag="PLU11_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59563.1"
                     /translation="MLQRFIIVLFCCLTGLSLSAQDMSGIWRGYFVTENYEHYKFELQ
                     IKQSGNSVSGVSYSYLSTVFYGKASLTGSYNKAGKKILIQEIRTVELRMSDGSAACIM
                     KCNFEYSRSGKEEFLEGTYTSKFEKDGLLTKKGENCGGGKVYLRRVANSDFYIEPFLR
                     GKVKPGPILINKAPVKSNPADKKTSPVLVNKAPVKKDTVKAKVNPPVVKKPPVVKNPP
                     VVTKPKPVIRKDTAVLKPVAGNLAKHDSAMRKVKPALITPAPPDIKNRINELVKTLTI
                     HSNQVTVKLFDNGEIDGDTISVYLDNKKVLSEKRLTAAPLVLRFDLSEQEDEHELTMV
                     AENLGTIPPNTSLMIVEAGTQRFEVRITSTEQKNAVVKFRYQKPG"
     gene            2123..2980
                     /locus_tag="PLU11_10330"
     CDS             2123..2980
                     /locus_tag="PLU11_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59564.1"
                     /translation="MARHFFLYILVFTGISLSAQNINGIWKGKVVMAPSGCFPVYQIE
                     LQLQLAGSHITGTAYHFSDSSNYLKENFEGTYNRDSNLITIQEIGIVTFKIKEDCVPC
                     IKTYKLSFHRGNGNVVTEEQLRGTWSTPTGKAIDGKTVCEPGSIVLTRFDKTLFKPEV
                     KLPPSLTNRKAELVKEIKVDTGNIKIDFYDNGQIDGDTISVYVNSMPLVSNRMLKTQP
                     ISVSVRVDQKKTVQEVIMVGENLGSIPPNTALMIITAGTKRYQLYLTSDEQKNAMVRF
                     VYEKPLTDK"
     gene            complement(3194..5476)
                     /locus_tag="PLU11_10335"
     CDS             complement(3194..5476)
                     /locus_tag="PLU11_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN59565.1"
                     /translation="MNGYVKDSLSGETIIGATVAVNGLSRGVTSNQYGFYSLTLDEGE
                     YDIQVSHVSYMGQVVPLHLHSNQTLNFDLLSKSALINEVVVYSKKRDGNVKNAQMGKI
                     DLTMNQVRAIPAFMGEVDILKAIQLLPGVQSAGEGNAGFYVRGGGPDQNLIMLDDAVV
                     YNTGHLFGFFSIFNGDAIKNVSLIKGGMPAQYGGRLSSVLDVSMKEGNINKFQTEGGI
                     GLIASRFSVQGPIKKEKASFIVSARRTYIDALVKPFVSKSSSFYGSGYYFYDLNAKVN
                     YRFSDKDRLYLSGYFGRDVFDFRNSKRSFNTNIPWGNSTATLRWNHVFNRRLFANTTA
                     VYNDYNFKFSAAQENFEIGLSSGIRDMTIKSDFDYYPLPNHKLKFGGLLTHHKFIPNV
                     VTGRQDSILFNPNNEGAKYAMEAAVYIQDDWEIGEKLKINYGIRWSSFNQVGPYMLYT
                     RDVNGNKLDSISYARLKTVKSYGGLEPRMTIRYALDDESSLKAAVTRNYQYIHLVTNA
                     GTTLPTDLWVPSTYIVRPQLSWLYSAGYFRNFADNRYETSVEVYYKDMRNQVEYKEGY
                     TPSLADPEEEFVFGKGWSYGAEFFVNKTRGRMTGWIGYTLSWTWRKFEQLNDGGRFPA
                     RYDRRHDISVVGNYDAGKKWKLGAVFVYGTGNAITLPERFYIINGVLTQEYSNLNQYR
                     MKAYHRMDFSATYTPVPKKKRKLTSSWVFSIYNVYSRLNPYFLYFDQEGSIATNDLQV
                     STKQVSLFPVLPAVTWNFKF"
BASE COUNT         1590 a         1282 c         1231 g         1486 t
ORIGIN      
        1 gcttcgtatt gcctgactgc accatccggc agggtaatat taatcatctg ttttacttgt
       61 ttttatcgga gagcaaaggt aacgaataat gcctaaagat gacgcagaat caggcggtca
      121 atcctttctt tgcagcgggt ggtttttctt ttcaaccggg cttctttttc aggatgggtg
      181 gattgccaga ttgccagccg gaattggcgt tttcgccacc gggacatcat aatcgatcca
      241 taggaccggg ctaagcctaa agtcagttta tccaaaagcc gggtaagacg tgtcgcttgt
      301 acgttctcca cataaaactg cttaaactgg ttcctgagcc cttcctgaaa ccggataaat
      361 gttttcttat tcgactgtag ttctttcacc gtaaataccc cggtcagggt ttcggacaga
      421 tctgcattga tatgggcatt aataccaagt aactggtatt gtaaaggggt caatgtactg
      481 tcgccgaaat acacctgcca ggcttccgat ccgtgtcccc gccccctgct agccgccgcc
      541 cggaaaaaat agccggcaaa agtctgctca aaccgttgaa taaattcttt ctcccgggca
      601 tcagcccttt gataatgctg aagggaaatc agtacggttt cggtataaag tttagcaaaa
      661 tgactggagg gtgcggcgga actggcaatt gaatccaact gaagggccag ccggattccg
      721 ggatcttttt cctgtgcctg caggcatagc ccactttgga gaagaacagt atagtaaagg
      781 aatcgaagag aatgcatatt tttaaaaacc tgttaaaatt aacaaactat tgcccggtat
      841 cgccatttca aacgatcttg atttcagtaa atttgcagac tgtcgaagcc tatgttacaa
      901 cgattcatta ttgttttatt ttgctgcctg accggcttgt ccctgtcggc ccaggatatg
      961 tctggtatct ggcggggcta ttttgtcacc gaaaattacg agcattacaa atttgaactt
     1021 cagatcaaac agtccggtaa ttccgtttcc ggtgtttcct actcctacct ctccactgta
     1081 ttttatggga aagcgtcact gaccggaagt tataataaag cgggcaaaaa gattctgatc
     1141 caggaaatca gaaccgtgga gttacgcatg tcagacggat cggctgcctg tatcatgaaa
     1201 tgcaattttg aatacagtcg atccggaaag gaagagttcc tggaaggcac ctataccagt
     1261 aaatttgaaa aagacgggtt gctgactaag aaaggtgaaa actgtggtgg aggtaaggtt
     1321 tacctgcgcc gggtggccaa ctccgatttt tatatagaac ccttccttcg cggcaaagta
     1381 aaacccgggc ctatactgat caataaagca ccggttaaat cgaacccggc tgataaaaaa
     1441 acatccccgg tcctggtgaa taaggcaccg gttaaaaaag atacagttaa agccaaagtc
     1501 aatccgccgg tagttaagaa acccccggtg gtaaagaatc cacccgtggt aaccaaaccc
     1561 aaacccgtta tccggaaaga tacggcggta ctgaagcctg tcgccggtaa cctggccaag
     1621 catgacagtg ctatgcggaa agtaaagccg gccctgatca cacctgcccc gccggatatt
     1681 aagaaccgga tcaatgaact ggtaaagacg ttaaccatcc atagcaacca ggttacggtt
     1741 aaactatttg acaacggcga aattgacggg gataccatct ctgtttacct ggataataaa
     1801 aaggtacttt ccgaaaaaag actcaccgct gcaccgctcg ttttgcgttt tgacctgagt
     1861 gaacaagagg atgaacatga actgacaatg gtggccgaaa acctgggaac cataccccct
     1921 aatacctccc tgatgattgt ggaagccggt acgcaacgat ttgaggtgcg gattacgtct
     1981 actgaacaga aaaacgcagt ggttaaattc aggtatcaga aaccaggata accggtaaga
     2041 cggactggat tccgggcaaa gaaaattgcc gctgctgtaa ttttttcagg accgcttctt
     2101 ttttccttat ttttccaccg ttatggcaag gcatttcttc ctttatatat tagtatttac
     2161 cggtatttca ttgtccgccc agaatattaa cggcatctgg aaagggaaag tggtgatggc
     2221 gcccagtggg tgttttcccg tttatcagat agaactccag ctccaactgg ccggcagcca
     2281 tatcacggga acagcctatc acttttctga ttcttccaat tatttaaaag agaactttga
     2341 aggaacttat aaccgtgaca gcaacttaat taccatccag gaaatcggta ttgtgacatt
     2401 taagattaaa gaggattgtg ttccctgtat caaaacctat aaactcagtt ttcaccgggg
     2461 caacgggaat gtggtgaccg aagaacaact acgtggtacc tggtctacac ccaccggtaa
     2521 agccattgac gggaagaccg tttgcgaacc ggggtccatt gtcctcaccc gttttgataa
     2581 gacgctcttc aaaccggaag tgaaactccc tccttcactc accaaccgga aagcggaact
     2641 ggtgaaggaa ataaaagtag acaccgggaa tatcaaaatt gatttttatg ataacgggca
     2701 gatagatggt gatacgattt ctgtttatgt aaacagtatg ccgttggtgt ccaaccggat
     2761 gctgaaaact caaccaatat ctgtaagtgt ccgggtggat cagaagaaaa cagtacagga
     2821 agtgatcatg gtgggagaaa acctgggctc cattcctccc aataccgcat taatgattat
     2881 taccgccggc acaaaacgtt atcaactcta tcttacttct gatgaacaga aaaatgccat
     2941 ggtccgtttt gtttatgaaa agccgttgac ggataaatga tctttattat cttttttgtc
     3001 gtagaatgac tacaaaaaag taagtatgaa taacaaacaa tttaaataac tggtaagaaa
     3061 gctaaatata ttaagctatt agtttgtgga gaatacttta ataggtaact ttagtatgca
     3121 aacaatttta atttcctcaa tttctttgac gcaagaaggc tccgttccaa cggggctttt
     3181 ttgtttaggg aaatcaaaac ttaaaattcc aggtgactgc tggtaataca gggaacaggg
     3241 atacctgttt ggtcgaaacc tgcagatcat tcgtggcgat acttccttcc tggtcaaaat
     3301 agagaaagta aggattcagc cggctgtaga cattataaat gctgaatacc catgaactgg
     3361 ttaatttccg tttcttttta ggtacaggcg tataggtggc cgaaaaatcc atccggtgat
     3421 aagctttcat ccggtattga ttcagattac tgtattcctg cgtcagtacc ccgttgataa
     3481 tataaaatct ctccggcaaa gtgattgcat tgccggtacc atacacaaat acagccccca
     3541 gtttccattt tttcccggca tcataattac caaccactga aatatcatgt ctccggtcat
     3601 aacgggccgg aaaacgcccc ccatcattta attgttcaaa tttgcgccag gtccaggata
     3661 aagtataacc gatccaaccc gtcatccgcc cccgggtttt atttacaaaa aattcagctc
     3721 cataactcca gcctttacca aaaacaaatt cctcttcagg atcggccagt gaaggcgtgt
     3781 atccttcttt atactctacc tggttgcgca tatccttata atacacttcc accgaggttt
     3841 cataccggtt atctgcaaaa ttcctgaagt aaccggcaga atacaaccag ctcaactgag
     3901 ggcggacaat atacgtactc ggcacccaca gatcagtggg taaagtggtg ccggcattcg
     3961 tcaccaggtg gatatattgg taattgcggg taaccgcggc tttcagggag gattcatcat
     4021 cgagtgcata ccggatcgtc atacggggtt ccagtcctcc ataggatttt acggttttta
     4081 aacgggcata agaaatactg tccagtttat ttccattcac gtcgcgggta tacaacatat
     4141 agggtcccac ctggttaaaa ctgctccaac gtattccgta gttgattttc agtttctccc
     4201 ctatttccca gtcatcctgt atatacacgg ccgcttccat ggcatatttg gctccttcat
     4261 tattagggtt gaaaagaatg gaatcctggc gaccggtcac cacattcggt ataaacttat
     4321 gatgggtgag cagacccccg aatttcagtt tgtgattggg taaggggtaa taatcaaaat
     4381 ctgatttgat tgtcatatcc cggatgccgg aggataaacc aatctcgaaa ttttcctggg
     4441 cagcggaaaa tttaaaatta taatcattgt atacagctgt ggtattggca aacaggcggc
     4501 ggttgaatac atgattccac cggagcgtcg ccgtggaatt tccccatggg atattcgtgt
     4561 tgaaagatcg tttggaattc cggaagtcaa aaacatctct gccgaaatag ccactcaggt
     4621 aaagccggtc tttatccgaa aagcgatagt tgaccttggc attcaaatca tagaaatagt
     4681 agcctgatcc gtaaaaactg ctgctcttac ttacaaaggg ttttaccaat gcatcgatat
     4741 aggttcgccg ggctgataca ataaaggaag ctttttcctt tttgatcggt ccttgtacgg
     4801 aaaaacggga ggcaatcaac ccgatccctc cttccgtctg gaatttattg atattcccct
     4861 ccttcatgga tacatccagc accgaggaga gccggccacc atactgggcg ggcataccac
     4921 ccttaatgag ggaaacattc ttgatggcat ctccattgaa aatggaaaag aagccaaaca
     4981 gatgcccggt attatacacc actgcatcat cgagcatgat caggttctgg tccggtccac
     5041 cgccccggac ataaaaaccg gcattaccct caccggcact ttgcaccccc ggcaacaact
     5101 ggattgcttt cagaatatcc acttccccca taaaagccgg tatggccctg acctggttca
     5161 tggtcagatc gatcttcccc atctgggcat tttttacatt cccgtccctt tttttactgt
     5221 acaccaccac ttcgttgatc agggcggatt tggagagtaa atcaaaattc aaggtctggt
     5281 tggaatgcaa gtgtagcgga actacctggc ccatatatga aacatgggag acctggatat
     5341 cgtattcccc ctcgtcgagg gtgagtgaat aaaaaccata ttgattgctg gttacccccc
     5401 gggacaggcc atttacggct accgtggctc caatgatggt ttcaccggaa agactgtctt
     5461 ttacataacc gttcaaagta tagcgggttt gggcggctat gcctgaagac agcattaaaa
     5521 gggagacaac gactacatga aaccgaaaca tattcactac aacaaattat ttaatgattg
     5581 gttgatcag
//
LOCUS       DAOPMW010000215         5588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16828_length_5588_cov_5.934574, whole genome shotgun sequence.
ACCESSION   DAOPMW010000215 DAOPMW010000000
VERSION     DAOPMW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16828_length_5588_cov_5.934574"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1540)
                     /locus_tag="PLU11_10340"
     CDS             complement(122..1540)
                     /locus_tag="PLU11_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN59566.1"
                     /translation="MKVEQIYTGCLAQGAYYIESNGEAAVIDPLREVAPYIQKAEINN
                     SSIKYVFETHFHADFVSGHIDLSQKTGAPIVYGPTAVPSFPAHIATDGEVFKIGAVTI
                     TAIHTPGHTMESTSYLLKDENGKPAGLFSGDTLFIGDVGRPDLAQKVVADLTQEILAG
                     HLYDSLRNKIMPLPDDLIVYPAHGAGSACGKNMSKETTDTLGHQKATNYALRADMTKE
                     EFIKEVTAGLVAPPAYFPLNVMLNIQGYESIDAVLKRGQQALSPNGFDIAATETNALV
                     LDTRDPQIFAAGFIPNSINIGIDGQFAPWVGAMIPDIKQEILLVTDEGREEEVITRLA
                     RVGYDFTIGYLKGGFNAWKAAGKEIDRIESIDAAELSRRMFHEKLTVLDVRKKSEYGS
                     EHLEESISAPLDFINESMTLIDKQKTAYVHCAGGYRSMIFNSILRARGYNKLVDVKGG
                     FKALKESGLFPVTDYVCPTTLL"
     gene            complement(1627..2424)
                     /locus_tag="PLU11_10345"
     CDS             complement(1627..2424)
                     /locus_tag="PLU11_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPN59567.1"
                     /translation="MEIIGYISAILIGISLGLVGSGGSILTVPVLVYLMSVDPLLATT
                     SSLFIVGFTSLIGGIRAYSKKQLDYRAVTEFGFPSVLSIFITRHNLLPAIPDIIFTTA
                     GITVTKNMLLMLVFAVLMLLASWSMIRSREGTNLVKAKEGGARVFPLMIFGLLIGVVT
                     GLLGAGGGFLIIPTLVLFLGLDMKTAVGTSLLIIAINSLFGFLFSLNHFTFNWILLLS
                     FTALAAAGLFIGSHFSDKISPATLKKGFGWFILFMGIFVIVRELFLS"
     gene            complement(2482..3105)
                     /locus_tag="PLU11_10350"
     CDS             complement(2482..3105)
                     /locus_tag="PLU11_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPN59568.1"
                     /translation="MNWKEQFPQFEPGLIELIEKEAVQRSFNAGDIIMRTGQYIKSTV
                     LVLEGRIKIYRENQDGGEFLMYYLQPGQACAVSMICAIQSGTSEIMAMAEEDSEVLMI
                     PVHLMDDMMNQYKSWYQFVIQTYRGRFDELLSVVDNIAFRNMDERLEFYIKRYVEKAG
                     KKSLEISHQQIADDLNSSREVISRLLKKMEQRGLVKLHRNMIELIPQ"
     gene            complement(3115..4395)
                     /gene="murF"
                     /locus_tag="PLU11_10355"
     CDS             complement(3115..4395)
                     /gene="murF"
                     /locus_tag="PLU11_10355"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPN59569.1"
                     /translation="MNISSLYELYLRNPSVQTDTRKLQPGDLFFALKGDNFNGNKFAK
                     QAIEAGAACAVIDEAAYAIDGKTFLVDDVLTALQELAKHHRQQFSIPFIAITGSNGKT
                     TTKELVHAVLSRKYRTATTEGNLNNHIGIPLTILKIKSDAEIAVIEMGANHQREIAGY
                     CTYTLPDYGIITNCGKAHLEGFGGVEGVRKGKGELFDWLREHDGTAFVMWDYDYLRDM
                     SQGLSHIITYGTTDASIEGHTIKSEPFLEVSITYGAATGTIHSQLVGDYNLPNLLLAV
                     TIGKHFSVPDEDIRKAIEAYQPSNSRSQLMQVGSNQIILDAYNANPSSMKLAIENFAR
                     LAAPHKVLMLGAMAELGAESLSEHRQIIELIGKFKWKEVVLVGGDFLNLQHHWISFPD
                     SAAAKQWYLDQHFEQTHLLVKGSRSMKMEKILEA"
     gene            complement(4658..>5588)
                     /locus_tag="PLU11_10360"
     CDS             complement(4658..>5588)
                     /locus_tag="PLU11_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPN59570.1"
                     /translation="TTKSYLLGLYTAPPGKAMQSKKGQPPMAPTMESGVMSPDYRLPF
                     EAEWEYAAYGLINQNPRPSLKEGKRGEELQSNKQVYPWSQNVNGLRDTRRGSWQGTFL
                     ANFKRGSGDNAGVAGGLNDRAFYTAPVNSFFPNGFGVYNMAGNVSEWVEDTYRPLSSL
                     DFDDQPAPFRGNEYKKLYVADPTTMDPATRYEKDSLGRVKMVNVTDEESKNRRNYQRG
                     NVISYLDGDSLSQSSYGYANTTLVHPEKSKVYKGGSWNDRAYWLTPGSRRHLEEDQAL
                     STLGFRCAMNRMGSPEGNKRKTGQYFKSRRQKR"
BASE COUNT         1393 a         1310 c         1338 g         1547 t
ORIGIN      
        1 gaatatgttc cattgcaaat tccatcgggc tgcgtacatc cacaatgatg gtggagggat
       61 ctttaaccag ttcttttaaa ttagccattt tatattgttt ttatttttat gtattcgttt
      121 attaaagtaa tgtggtcgga caaacataat cagtgaccgg gaacaatccg ctttctttca
      181 gggctttgaa accgcctttc acatccacca gtttgttata tcctcttgcc cgcaggatcg
      241 aattaaagat catagagcgg taaccaccgg cacaatgcac ataggcagtt ttctgtttat
      301 cgatcaacgt catactctca ttgataaaat cgagcggagc gctgatggat tcctccaggt
      361 gctcactgcc gtattcactt tttttccgga catccagcac ggtcagtttt tcatgaaaca
      421 ttctacggga taactcggct gcatcaatgg attcgatgcg gtcaatttct tttccggctg
      481 ccttccaggc attgaatcct cctttcagat agccgatcgt aaagtcgtaa cccacacggg
      541 ccagccgggt gattacttct tcctcacggc cctcatcggt tacgagcagg atctcctgtt
      601 tgatatcagg aatcatggcc cccacccagg gagcaaattg tccgtcaatc ccgatattaa
      661 tggagttggg aatgaaaccg gctgcaaaaa tttgcggatc acgggtatcc agcaccagtg
      721 cattggtttc tgttgctgct atatcaaatc cgttcgggct cagggcctgt tgtcctcttt
      781 ttaaaaccgc atcaatgctt tcatagccct gaatattcaa catgacattc aacgggaaat
      841 aagcaggggg tgctacaaga ccagccgtaa cttcttttat gaattcttct ttcgtcatat
      901 cggcccgcaa tgcataattg gtggctttct gatgaccgag tgtatcggtg gtttccttac
      961 tcatgttttt tccgcaggca ctgccggctc catgggccgg gtaaacaatc aggtcatccg
     1021 gcaggggcat gattttattc cgcagggaat catacagatg cccggctaat atttcctggg
     1081 taaggtcagc caccactttc tgtgcaaggt cgggacgacc cacatcgccg atgaataaag
     1141 tatcaccgga gaaaagcccg gctggctttc cattttcatc ctttaataaa tagctggtgc
     1201 tttccatggt atgcccgggt gtatggatcg ccgttatcgt gaccgccccg attttaaaca
     1261 cttcaccatc ggtggcaata tgagccggaa aagaagggac tgctgtcgga ccatacacga
     1321 tcggtgcacc ggtcttttgg gacaagtcaa tatggccgct tacaaaatca gcatggaaat
     1381 gggtttcaaa aacatattta atgctggaat tgttaatctc cgctttttga atatagggag
     1441 ccacttcacg gagcggatcg atcaccgctg cttctccatt actctctata tagtaagcgc
     1501 cctgtgccag gcagcctgta tatatctgtt ctactttcat agcttccatt tttaattgtg
     1561 aatacaaaaa tcaaatttta gcgggaacta tacagtgaca tccatcacag taggttggag
     1621 ggcggttcag gataaaaaaa gttccctgac aatcacgaaa atacccatga aaaggatgaa
     1681 ccagccaaaa ccttttttca gcgtggcggg acttatttta tctgagaaat gactgccaat
     1741 aaaaagaccg gcagcagcaa gtgcggtaaa ggaaagcagc agaatccagt tgaaggtgaa
     1801 atgattcagg ctgaataaaa aaccgaaaag ggagttaata gcaatgatca gcagggaggt
     1861 tcctacggct gttttcatat ccagcccgag gaataatacc agggtgggga tgatcaggaa
     1921 cccgccaccg gcaccgagca gacctgttac cacaccgatc agcagaccaa aaatcatcag
     1981 cggaaacacc cgggctccgc cttcttttgc cttgacaagg ttggtgcctt cccggctacg
     2041 gatcatggac caggaagcca gcagcatcaa taccgcaaag acgagcatca gtaacatgtt
     2101 ctttgttaca gtgatgcccg ctgtagtaaa aatgatatcg ggtatcgccg gtaacaaatt
     2161 atgccgggta atgaagattg aaagaacaga tggaaatccg aattcggtta cggccctgta
     2221 atccagttgt ttttttgaat aggccctgat accgccgatc aggctggtaa atccaactat
     2281 aaagagggaa ctggtagtgg ccagcaaagg gtccacactc ataaggtaaa ccaatacggg
     2341 tacagtcaga atcgagccgc cgctgcccac caggccaagg gatatgccaa ttaagatggc
     2401 ggatatgtaa ccaataatct ccatgaatat tttgcaagat tagctgaata gaaacggccc
     2461 gggtgtgaca gaaatcacgg gttactgggg gatcagttca atcatattcc ggtgcagttt
     2521 caccaatccg cgctgttcca tctttttcaa taaccgggaa atcacttcac gggaactgtt
     2581 caggtcatcg gcgatttgtt gatgcgaaat ttccaggctt ttctttcctg ctttttccac
     2641 atagcgttta atataaaatt caagccgttc gtccatattg cgaaaggcaa tattatccac
     2701 cacggataaa agttcgtcaa accgtccacg gtaggtctgg atcacaaact ggtaccagct
     2761 cttatactgg ttcatcatat cgtccatcag gtgtaccggg atcatgagca cttctgaatc
     2821 ttcttcggcc atggccataa tttcactggt tcctgattgg atcgcacaga tcatcgatac
     2881 cgcacaggcc tgaccgggtt gcaggtagta catgagaaac tcacccccgt cctggttttc
     2941 gcggtagatt tttatccgtc cttccagcac caataccgtg gatttgatgt attggccggt
     3001 acgcataatg atatcgccgg cattaaaact gcgttggacc gcttcctttt cgatcagttc
     3061 gatcaggccg ggttcaaatt gggggaattg ctctttccag ttcataagac aaagttaggc
     3121 ttctaaaatc ttttccatct tcatactcct gcttcctttc accaggagat gggtttgctc
     3181 aaaatgctga tcaagatacc attgtttggc tgctgctgag tcgggaaagg agatccagtg
     3241 atgttgcagg tttaagaaat ctccccctac caataccact tctttccatt tgaattttcc
     3301 gatcagttca atgatctgcc ggtgttctga caggctctct gctcctaatt ctgccatggc
     3361 acccagcatc agtactttgt ggggggcggc cagacgggca aagttttcaa tcgccagttt
     3421 catgctgctg ggattggcat tataggcatc aaggataatc tggttgcttc ccacttgcat
     3481 taattgggag cggctgttgg aaggttggta ggcttcaatg gctttacgga tatcctcatc
     3541 cggtacgctg aaatgtttac caatcgttac ggccagtagt aaattcggga gattgtaatc
     3601 acctaccagt tgtgaatgga tggtaccggt agctgcgccg tacgtaatag agacttccag
     3661 gaagggttcg ctttttattg tatgtccttc gatactggca tcggtggtac cataggtgat
     3721 gatatgggat aatccctggc tcatatcgcg gaggtagtca taatcccaca tcacaaaagc
     3781 ggtgccgtca tgctcgcgga gccagtcaaa caattctcct tttcctttcc gtacaccttc
     3841 cactccgcca aaaccttcga gatgggcttt gccgcaattg gtgatgatgc cgtaatccgg
     3901 cagggtatac gtgcaatagc cggcaatttc ccgctggtga ttggcgccca tttctatgac
     3961 cgcgatttct gcatcggatt ttatttttaa aatggtgagg ggaataccaa tatggttatt
     4021 gagattgcct tcggtggtgg cggtgcggta tttgcggctc agtacggcat gtaccagttc
     4081 tttggtagtg gttttgccat tactgcctgt aattgcaata aatggtatgg agaactgttg
     4141 ccggtgatgt ttcgcgagtt cctgtaaagc ggtcagcaca tcgtcgacca ggaaggtctt
     4201 tccatcaata gcatacgccg cttcatcgat caccgcacag gcggctcctg cttcgatagc
     4261 ctgttttgca aatttgtttc cgttgaagtt atctcccttt agtgcaaaga ataaatcgcc
     4321 gggttgcagt ttacgcgtat ccgtttgcac ggaggggttg cgcaggtaga gttcgtagag
     4381 ggaggagatg ttcattttgt aaaagtagta aatgaagttg gaggttggag gttggaagtt
     4441 cgaggttgga ggttcgaggt tggaagttgg aggttcttaa agcagaaaaa tcctaactgt
     4501 ctttaaaatg gttgattatt gtaccattta aagagagcgc cagactgtaa tgaagcctcc
     4561 ctcatcacga aacctccaac ttcgaacttc caacctcgaa cctcgaacaa aaaaaaccct
     4621 ccatattgtg gagggttttc ttataagttc acttagacta tcttttttgt cttcttgatt
     4681 tgaagtactg acctgttttg cgtttgttac cttcggggct tcccatgcgg ttcatcgcgc
     4741 aacggaatcc gagggtgctg agggcctggt cttcttcgag gtgacggcgg ctgcccggtg
     4801 ttaaccagta agcgcggtca ttccagcttc ctcctttgta cactttggac ttttccgggt
     4861 gtaccagggt agtattggca tagccataag aagattggga taaggagtca ccatcgaggt
     4921 agctgatcac attaccgcgc tgatagttcc ggcggttttt gctttcctca tcggttacat
     4981 tcaccatttt cacacgaccc aggctgtctt tctcatagcg ggtggcggga tccatggttg
     5041 tggggtctgc tacatataat ttcttgtatt cgttaccacg gaacggagct ggctggtcgt
     5101 caaagtcaag tgaagaaagc ggacgatagg tatcttccac ccactcactt acgttaccgg
     5161 ccatattata tacaccgaaa ccattcggga aaaatgaatt aacaggagcg gtgtagaaag
     5221 cgcggtcatt cagaccaccg gccacaccag cgttatcacc tgaacctctt ttgaagttgg
     5281 ccaggaaggt accttgccag ctgccacgac gggtatcacg caatccgttc acattctgtg
     5341 accaggggta aacctgtttg ttggactgta actcttcccc tctcttacct tctttcaggc
     5401 tgggacgggg gttttggttg atcagaccat aagcggcata ttcccactcg gcttcaaaag
     5461 gaaggcggta atcaggcgac atcacaccag actccatcgt aggagccatc ggcggctggc
     5521 cttttttaga ctgcatggct ttgccgggag gggctgtata caaacctaac aggtaggatt
     5581 ttgtagta
//
LOCUS       DAOPMW010000216         5559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16942_length_5559_cov_7.502725, whole genome shotgun sequence.
ACCESSION   DAOPMW010000216 DAOPMW010000000
VERSION     DAOPMW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16942_length_5559_cov_7.502725"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLU11_10365"
     CDS             <1..446
                     /locus_tag="PLU11_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN59571.1"
                     /translation="FGKISTGAQNDLQQITRIAYSMVTVYGMNSKVGNVSFYDPQQET
                     AFTKPYSDETARMIDEEVRKLIDEAYEKTKDLLTMRKGEVEKLAQALLDSEVLFQSDV
                     EALIGKRPYEEKKSLGVDETDDQPVLGESAISNPFPLDGHSTTGA"
     gene            476..1111
                     /locus_tag="PLU11_10370"
     CDS             476..1111
                     /locus_tag="PLU11_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPN59572.1"
                     /translation="MNVSPSKENILKKIRKALSHSTPLPFPQSEGSGTVYQPLQQEAE
                     IEFAEQFTKLQGKFIFCINRQELAFQLGSLVRKQDWQKVYCVEEVLIDSFTPQIEDRL
                     VKTDLAHCDVAITSCEYLVARTGSIVLSTSTASGRSTSVYAPIHICIAYTSQVVYDLK
                     DALQAVKDKYGNNLPSLITFASGPSRTADIEKTLVVGVHGPKEVYLFLVEA"
     gene            1138..1563
                     /locus_tag="PLU11_10375"
     CDS             1138..1563
                     /locus_tag="PLU11_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPN59573.1"
                     /translation="MTSHTIHNLFVYGSLRSGFQSHAYEYISRFFTLVGPAKVKGKLV
                     DMGAYPAGIPTDEERFIYGELYQIKNANEFSWAMGQLDDYEGVNAEEEHERLYGRAIT
                     EVIAGDMTLEAWIYWYLGDTSGQPVIESGDILLYMKDKQ"
     gene            1622..2407
                     /locus_tag="PLU11_10380"
     CDS             1622..2407
                     /locus_tag="PLU11_10380"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPN59574.1"
                     /translation="MTITSGKKIYFLSDFHLGAPDHASSLERERKIVQFLDEIKDQAA
                     EIFFVGDMFDFWYEYRTVVPKGFVRLLGKIGELSDAGIPMHFFVGNHDMWMKNYFQQE
                     FNMPVYYAPVEFERNGKKFLIGHGDGLGPGDHGYKRLKKVFRNPVSQFLFGIFPPMLG
                     MGLANYMSRRSRAQTGTREEVFLGEDKEWLVIYCKEKLKQKKSDFFVFGHRHLPIDFR
                     LNESSRYINLGDWIRYFTYAEFDGQDMELKSYTGMEQNIIRKG"
     gene            2404..3237
                     /locus_tag="PLU11_10385"
     CDS             2404..3237
                     /locus_tag="PLU11_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59575.1"
                     /translation="MKQLSVTILIICCTAFMGRAQTDTSFRLLRSIKGDIVHFTIDNL
                     DNIYVLNSQNQVKKYNANGDSVAIFNDIRKYGRASLIDVSNPLKVLLYYRDFATVVML
                     DRFLNPVNTVDLRKENIFQAKTIAQSYDNKIWVYDEVENKLKKIGEDGKLIQETPDFR
                     LLLDQSVNPVKISDENQYVYLYDSTKGVYVFDYFGSLKNVIQIRKWKNLKVAGNYIFG
                     SLGNTLYRYEISSFLYDEWTLPAELSSATVFNFSNSRLYALRPEADGKSAVIAIYTLR
                     R"
     gene            3283..4371
                     /locus_tag="PLU11_10390"
     CDS             3283..4371
                     /locus_tag="PLU11_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPN59576.1"
                     /translation="MNDFLNEVWGGNPIRSYLTVAAVILFVWLFRSFISRYFAGLLYG
                     IVHRIWKNVDKKAFTSLVLKPMGFFLLILVSVAALYKLNFPEELNETIYRYSIKEILQ
                     SLGTIILIVSLFELLLRLIDFIALILERKANLTPDQSDNQLIVFFKDFFKVILVIIGI
                     MLVLRFAFSLDVGGLLTGLSIVGAAIALALRESLENLIASFIIFFDKPFITGDLVKVN
                     AITGTVERIGLRSTRIRTEQKSYVTVPNKQMVDSILDNLSLRTQRRGDLKLEIGLQTP
                     PASIEALLEGIRKIVGREEVEEYNVNLSEITSQAIVIVSDYYTAPIGIKEFFTIRQEI
                     NLAALKLMEELKIEIAGASTDVKLVSSR"
     gene            4807..5373
                     /locus_tag="PLU11_10395"
     CDS             4807..5373
                     /locus_tag="PLU11_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59577.1"
                     /translation="MKYLYLLAFFFSTSNLESGCKKTNNLIIWDQHYQLTDDAFKSKE
                     PAYLNTLAESFIGLRVEYEYRDTLVFDVLCTMDKSKSWMIENSIYVLKHEKGHFDIGE
                     IYARILRKSIFSVKKSIKPANFKIIDSLYYDIGKKMLDEQRKYDTATTIPARNREAQS
                     AWNLSIQKRLDSLDGYKRNKSEKLRLKR"
BASE COUNT         1563 a         1108 c         1386 g         1502 t
ORIGIN      
        1 tctttggtaa aatttccacc ggtgcacaga atgacctgca gcagatcacc cggatcgctt
       61 attccatggt gacggtctat ggtatgaata gtaaagtggg taatgtgagt ttctatgatc
      121 cgcaacagga aaccgctttc accaaaccgt attctgatga aacggccaga atgattgatg
      181 aagaagtgag aaagctcatt gatgaagcat atgaaaaaac aaaagacctg ctgacgatgc
      241 gaaaagggga agtggaaaaa ctggcccagg cattgctgga cagtgaagtg ttattccaga
      301 gtgatgtgga agccctgatc ggtaagcgtc cttatgagga aaagaagtca ctgggtgtgg
      361 atgaaacaga tgatcagccg gtattaggtg aatccgctat ttccaatcca tttccgttag
      421 atggccattc aacaaccggt gcctgattgg cgggccggtt ttataaaact gttgtatgaa
      481 cgtttctcca tcaaaagaaa acatacttaa aaaaatccgc aaggcgttga gtcactcaac
      541 gcctttgcct tttccgcaaa gtgaaggaag cgggaccgta taccagccct tgcagcagga
      601 agcggagatt gaatttgccg agcagtttac caaactgcag ggtaaattta tattctgtat
      661 caaccggcag gaactggctt ttcagctggg cagcctggtg cgtaaacagg actggcagaa
      721 agtatattgt gtggaggagg tattgattga ttcttttacg ccccagatcg aagaccggct
      781 ggtgaaaacg gacctggcgc attgtgatgt agccattacc agttgcgaat acctggtggc
      841 ccgcaccggt tcgattgtac tcagtacttc cacggccagt ggccggagta ccagtgtgta
      901 tgcgcccatt catatctgta tcgcgtatac cagccaggtg gtgtatgacc tgaaagacgc
      961 cttgcaggca gtgaaagata aatacgggaa taacctgccc tcgctgatca cctttgcttc
     1021 cggacccagc cgtaccgccg atattgaaaa gaccctggtt gtgggggtgc atggtcccaa
     1081 agaagtctat ttattcctgg ttgaagcata agtcagcccc cttttgtatt tttaggtatg
     1141 actagccata cgatacataa tctttttgtt tacggttcat tgcgcagtgg tttccagagc
     1201 catgcctatg aatatatcag ccgttttttt accctggtag gacctgcaaa agtaaaaggc
     1261 aagctggtgg atatgggtgc ctacccggcc gggattccaa ctgatgaaga acgtttcatt
     1321 tacggggaac tttaccagat aaagaatgcg aatgaatttt cctgggccat gggtcagctg
     1381 gatgactatg aaggggtgaa tgcggaagaa gagcatgaac gtctctatgg ccgtgcgata
     1441 accgaggtga tcgccggcga tatgaccctg gaggcctgga tctattggta tttgggtgat
     1501 accagcggac agccggtaat tgaatccgga gatattcttc tgtatatgaa agataaacag
     1561 taatcagcat caggaattta cagccctgta cttcttcgat caataatcct gacaaaggca
     1621 catgacaatt acatccggaa aaaaaatata tttcctttcc gattttcatc tcggtgcccc
     1681 cgatcatgcc agcagcctgg aacgggagcg gaaaatcgtg cagttcctcg atgagatcaa
     1741 agaccaggcg gctgaaatat ttttcgtggg tgatatgttt gatttctggt acgagtaccg
     1801 gaccgtggtg cccaagggct ttgtccgcct gttgggaaaa atcggtgaat tatcagatgc
     1861 tggcatcccc atgcattttt ttgtgggcaa ccatgatatg tggatgaaaa attatttcca
     1921 gcaggaattc aatatgcccg tgtactatgc accggtggag tttgaaagga atgggaagaa
     1981 attcctgata ggtcatggag acggactcgg tcccggggat catggctata aacgactcaa
     2041 gaaggtattc cggaatccgg tcagccagtt tctttttgga atattccccc cgatgcttgg
     2101 catgggatta gctaattata tgagcaggcg aagccgggca cagaccggta ccagggaaga
     2161 agtcttcctg ggtgaagaca aggaatggtt ggtgatttac tgtaaagaaa aactgaagca
     2221 aaaaaagtca gatttttttg tgttcgggca tagacactta ccgattgatt ttcgtctgaa
     2281 tgaaagcagc cggtatatca acctgggcga ctggatccgt tatttcactt acgcagagtt
     2341 tgacggccag gatatggaac ttaaatcgta taccggcatg gaacaaaata ttatccggaa
     2401 aggatgaagc aactgagtgt aaccatattg atcatttgtt gtaccgcctt tatgggacgg
     2461 gcgcaaacgg ataccagttt ccggttgctc cggtcgatca aaggggatat tgtgcatttt
     2521 acgattgaca acctggataa tatctatgtg ctcaacagcc agaaccaggt gaagaaatac
     2581 aatgcgaatg gcgattcggt agccattttc aatgatatcc gtaaatacgg aagagccagt
     2641 ctgattgatg tgtcgaatcc gctcaaggta ttattgtatt accgcgactt tgctacggtg
     2701 gtgatgctcg accgtttcct caaccccgtg aatacggtgg atctgcgtaa agagaatatt
     2761 ttccaggcaa aaacgattgc ccagtcttat gataataaaa tatgggtgta tgatgaggtg
     2821 gagaacaagc tgaaaaagat cggggaggat gggaaactga tccaggagac cccggacttc
     2881 cgcttattgc tggatcagtc ggtcaacccg gtgaagatct cggatgagaa tcagtatgtc
     2941 tatctctatg actccaccaa gggggtttat gtctttgatt atttcggttc cctgaaaaat
     3001 gtgatccaga tccggaaatg gaagaacctg aaagtggccg gtaattatat cttcggttca
     3061 ctaggtaata cactttaccg gtatgagatc agcagtttct tgtatgatga gtggacgctg
     3121 ccggctgagc tcagttccgc cacggttttt aatttcagca acagtcgctt atatgcgttg
     3181 cgtccggaag cggatggcaa atccgctgtg attgccattt atacccttcg tcgctgagcg
     3241 atgtccttcc agtttaataa ttttgtggtt taatttactg ttatgaatga tttcctgaac
     3301 gaagtgtggg gaggtaatcc gatacgcagt tacctgacag tggcggcggt gatccttttt
     3361 gtatggctgt ttcgttcctt tatttcccgc tattttgccg gattacttta tggtatcgtg
     3421 caccggatct ggaagaatgt agataaaaag gccttcacca gcctggtgtt aaagcctatg
     3481 ggttttttcc tgctgatcct ggtgtctgtg gccgctttat ataaactgaa ttttcccgaa
     3541 gagctcaatg agacgatcta tcgttacagt atcaaagaga tcctgcagag cctgggtacc
     3601 atcatcctga tcgtttccct gtttgagttg ctgctccggc tgattgattt tatcgccctg
     3661 attctggaaa ggaaagcgaa cctgacgccg gaccaatccg ataaccagct catcgttttc
     3721 ttcaaagact ttttcaaagt catcctcgtg atcatcggca tcatgctggt cctgcgtttt
     3781 gctttcagcc tggatgtggg aggtttactt accggtctga gtatcgtagg tgcggccatt
     3841 gcccttgctc tgcgggaaag tctggagaac ctgatcgctt cctttatcat cttctttgat
     3901 aaacccttta tcaccggcga cctggtaaaa gtgaatgcta ttaccggtac agtagagcgg
     3961 attggcttgc ggagcacacg tatccgtacc gagcagaaga gttatgtcac tgttccgaat
     4021 aaacaaatgg tggattccat actcgataac ctttcgttac gcacccagcg ccgcggcgat
     4081 ctgaaactgg agatcggatt acaaaccccg ccggcctcaa ttgaagcatt actggaaggc
     4141 atccgtaaga tcgtgggtag agaggaagtg gaggaataca atgtcaacct gtccgagatc
     4201 accagtcagg ccattgtgat tgtatccgat tattatacag ccccgatcgg catcaaagaa
     4261 ttctttacca ttcgtcagga gattaacctg gctgcgttga agctgatgga agaattgaag
     4321 attgaaatag cgggtgctag tacagatgtg aaactagtta gtagtcggta gttagtagtc
     4381 gggagtcagt agtagtcgtt gtcaccctga gcgtgccgaa gggtgaagta gggagtggtg
     4441 agtagttgtg ggacacggat aaagagatat tacctgcggt aataggggat gaacacggat
     4501 ggatacggat tagggtgaaa caatatagtt aaaagcgacc agctcatgga agagatatat
     4561 aggcccgaag ggctgataca attatagcaa gaagttccta agatgcctat gagccccgaa
     4621 ggggtgacag atcaagtgat cgtgaataac aaacaatgag gattttgaag gcctgcggca
     4681 ggaaagttgg ccactccttt cgcgccttag cggggaaagc atacaaattg gcactaaaaa
     4741 gcccgacctg ccgaccgaaa cgcagtggag gtaggagggg tgaaatgata aagacttctg
     4801 aactgaatga aatatttata ccttttagct ttctttttct caacatccaa tctggaatca
     4861 ggttgtaaga aaaccaataa tttaataatc tgggatcaac actatcaact gactgacgac
     4921 gccttcaaga gtaaagagcc ggcttattta aacactcttg cggaaagctt cattggactg
     4981 agggtagaat atgaatacag ggatacgtta gtttttgacg tgttatgtac aatggacaag
     5041 tcgaaatcat ggatgattga aaattcaata tatgtattga aacatgaaaa agggcatttt
     5101 gatattggag aaatatatgc acgaatatta cggaaatcga ttttctctgt aaagaagagt
     5161 attaaacctg cgaattttaa aataattgat tcattgtatt atgatattgg caagaagatg
     5221 cttgatgagc aaagaaaata cgatactgca acaacaattc cggcaagaaa tcgagaagca
     5281 caatcagcct ggaatttaag tattcaaaaa agacttgatt cacttgatgg gtataagcgg
     5341 aataaatctg aaaagctcag acttaagcga taagggtgtg caaaaatgtg aaaatccccc
     5401 tttttttcat attcttttat acatttaaat agctcaagtt tacaattagc aattttccaa
     5461 tgcgacatca aacgctttta tatttctgtg tttttctttt acctttaagt tgcatagaag
     5521 aacccaagaa taagatattc attaaatctg ttgcatctt
//
LOCUS       DAOPMW010000217         5554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_16960_length_5554_cov_7.545736, whole genome shotgun sequence.
ACCESSION   DAOPMW010000217 DAOPMW010000000
VERSION     DAOPMW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_5554_cov_7.545736"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(968..3505)
                     /locus_tag="PLU11_10400"
     CDS             complement(968..3505)
                     /locus_tag="PLU11_10400"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPN59578.1"
                     /translation="MKPTLLAFCLLLFAQCVLAQVKKPVTPVKTKPVIDSVKALRDST
                     WKAQTKNKQKTEGLFTFYQDTVSGSATMYIRKDQLNKEFIYQSFSMGGPALLFLNQNM
                     IRETWVFKMRKVYGRMEFMRCNTNYYYDSTNAVSKAKDVDVSDAVFHTGAVRAEDSLG
                     YLINADSLFISEKLDMVKPILPPTVPPTAVFNLGNLVTDKSGFEKIRSFPQNSDVVVS
                     LAYDNPAPMNGGDRSITDARYVRVRMQHSFIKMPENNYQPRFDDPRVGYFTTESDNLT
                     SLKSTRYHDLIHRWNLEKKDPTAKLSEPVEPITWWIENTTPVELRQLFLEAGARWNEA
                     FEYAGFKNAVVMKMMPDTASWDPADIRYNVIRYVASDLGFAIGPSFVNPRTGQILGAD
                     ITMDHGFYKGILDEQSVFKQGTHPAGMNHKNFSSCTIASGMMKEFAAGYVFAELSGMP
                     AIELTHLREQFYTELVMHEMGHTMGLQHNMKASQMLSPAELLDTAITRKWGVIGSVMD
                     YSAVNVAPDLKPVDYYTTKTGPYDWWAIEFGYRPFNKGEETAGLKKILERSTEPKLIF
                     GNDADIASDHTGIDPRVQVWDMSSDMASYATGRFQLVNNLMGKLKDKYAVTGESYVDL
                     RRKYNALFGQRFGMAVGLSYYVSGVMVDRSFVGQQTSAKPFTPVPDEYKRKVLSALGT
                     YVFAPNAFDSDASLYPYLQMQRRGYNFGDGTEDPKPENDVMALQNTVLSGILNNTALA
                     RASRSTLYGNGFTPAVILNELSSLIFDADLKTDVNLYRQNLQYAYISKLISMMKDPAY
                     DHASASASFDNLKQLRAKMDKLNTGNEQTKAHRERLKFMIDKALVVK"
     gene            3888..>5554
                     /locus_tag="PLU11_10405"
     CDS             3888..>5554
                     /locus_tag="PLU11_10405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPN59579.1"
                     /translation="MKRLWFSLFLILVYNTLPAQPSSLVNPFIGTGGHGHTFPGATMP
                     FGMMQLSPDTRLEGWDGCGGYHYSDSAIYGFSHTHLSGTGIADYCDVLLMPFTGEVKW
                     KNKEYASPFSHKNEKASPGYYEVLLDKFNIRAALTTSLCSGMHQYTYDASAAEGKVLL
                     DLQHRDEVLGSSLELVNEYEVKGSRISKSWAKEQHLFFYIRFEKPIKRYRMALDDQPV
                     NGLNKLEGKNIKSYFSFDLGEEKIVRLRIGISGVSTENAKQNLEQEISHWDFNRLKQQ
                     AENAWNNELGKIEVKGGTHDQQVAFYTALYHTMIAPNIYTDVNGEYRGTDLKVHKADG
                     FTNYSVFSLWDTHRAQHPLQTIINKQRTNDWIKTFLAQYKAGGMLPVWELSGNETFCM
                     IGYHAVPVIVDAWKKGVQWFDKELALKAMIDYAESSRFGLPAYIKQGYVSNEDDHEST
                     SKTVEYAYDDWCISEFAAQIGNTKISEQYRKRALNYRNLFDPRVKHIRGKVQGFWYSP
                     FDATEVNNFFTEGNSWHYSFTAQQDIDGLKKLYGGQAGFAKQLNELFT"
BASE COUNT         1441 a         1291 c         1347 g         1475 t
ORIGIN      
        1 cacccgggag gcgctgtatg atctgaattt actcgcatcc gatcgtttgc atccatcgaa
       61 gattgaatac aggaagtggg cggttaggtt gatgccgttg gtacaagcag tagttagata
      121 gtagttagta gtcggtagtt agtagcctgc ctgccggtag gcaggtcaac agcccgttct
      181 gaggaggttt ttgtttaagc ctgaagggct ggtattatta tagaaacagt tcggttagaa
      241 aacttgtcac cccgaccgga gggaggggta gaacggccag taataaaatg gatatagatt
      301 ccgccgcagg aggaatcttg tatagaaacg aaattgcgta gaacagtcag atccctccct
      361 tcggtcggga ttgatgtggt attagttatt ggttgtctta tccctccttc ccttcgataa
      421 actcagggta aactccggga tgacaaaaca gccccttcta agaaaagata acttacacaa
      481 cgtactgatc ctcgttttat ggaatccggt tcatcccgat acttcgtccc gagatttcgg
      541 gattctaacc tcgtacttaa aaaatacaag gttcctcgtt agtcggaatg atcataataa
      601 ccggatgatc tcagccccga aggggtgtta caattatagc ccggcaagaa taaatgtgcc
      661 cattagcccc gaaggggtga cacaattgta ataaagaatc cttccataga tccctcgcta
      721 caatcaaata cattgttcgt tgcaaatccg gaatgaaggt aacgaaacaa ggttcgtcgc
      781 tcattgggaa tgacggcaac aatacaaggt ccctcacttc gttcggaatg acggcaaggt
      841 tggggagaga agagggaaat acaagatttc tcgctgcgct cgaaatgcca gcacaacctc
      901 ttcatttaaa aacaaagctc tcgttttacg aaatccaatt cgtacttcgt acttctaacc
      961 tcgtacttta ctttaccacc agcgctttat caatcataaa cttcaatctt tcccggtggg
     1021 cttttgtttg ttcgttgccc gtatttagtt tatccatttt tgcccgcagt tgcttcaggt
     1081 tgtcaaagga tgctgaagcg gaagcatgat cataggcagg gtccttcatc atactgatga
     1141 gtttgctgat ataggcatat tgcaggttct gacggtaaag attcacatcc gttttcaggt
     1201 cggcatcaaa gatcaggctg ctgagttcat tcaggatcac ggcgggggtg aagccattgc
     1261 catacaaggt ggaacggctg gccctggcta aagctgtatt gttcaggatg cctgataaaa
     1321 ccgtgttctg caaagccatt acatcatttt ccggtttggg gtcttcggta ccatccccga
     1381 aattataccc acgacgttgc atttgcaggt aagggtataa agaagcatcg ctgtcaaagg
     1441 cattgggggc aaatacatag gtgcccaggg cagacaatac ttttcgttta tactcatccg
     1501 gtaccggggt aaagggtttt gccgatgtct gttgtccgac aaaactccgg tcgaccatga
     1561 ccccgcttac ataataggat aatcccacgg ccatgccaaa gcgctggcca aagagtgcat
     1621 tatacttccg gcgcagatca acatagcttt cgccggttac tgcatattta tctttcagct
     1681 tacccatcag gttattcacc agctggaaac ggcctgtggc ataactggcc atatcactgc
     1741 tcatatccca tacctgtacc cgcggatcga tcccggtatg atcagaggcg atatccgcat
     1801 cattcccaaa aatcagtttc ggttcggtac ttctttccag tattttttta agccctgctg
     1861 tttcttctcc cttgttaaag ggccggtatc caaactcgat ggcccaccag tcgtaaggac
     1921 ctgttttggt cgtataataa tccactggtt tgaggtccgg tgcaacattc accgcggagt
     1981 aatccatcac agaaccaatg acgccccatt tacgggtaat ggccgtatcc agtaattcgg
     2041 ccggactgag catctggctg gctttcatat tgtgctgcag tcccatggta tgacccattt
     2101 cgtgcataac aagttctgta tagaactgct cgcggagatg ggtcagttca atggccggca
     2161 taccggagag ttcagcaaat acatagcctg cggcaaactc cttcatcatg ccactggcta
     2221 tagtgcagga gctgaagttt ttatggttca taccggccgg atgagtcccc tgtttaaaaa
     2281 cagactgctc gtccaatatg cctttgtaga aaccatgatc catagtgata tcggcaccta
     2341 agatctgccc ggtacgcggg tttacaaaac tcggaccgat ggcaaaaccc aggtcggaag
     2401 ccacataccg gatcacattg tagcggatat cggcgggatc ccagctggcg gtatccggca
     2461 tcatcttcat caccactgcg tttttaaatc cggcatattc aaaggcttca ttccaccggg
     2521 caccagcctc caggaagagc tgccttaatt ccaccggcgt ggtattctcg atccaccagg
     2581 tgattggctc cactggttcg ctgagtttgg cagttggatc ttttttctcc aggttccagc
     2641 gatggatcag gtcatgatag cgcgttgact tcaggctggt taaattatct gattcggtgg
     2701 tgaaataacc aacacgggga tcatcgaacc ggggttggta attattctcc ggcattttaa
     2761 taaaactatg ctgcatcctg acccgcacat atcgggcatc tgtgatcgac cgatcgccgc
     2821 cgttcatagg agcgggattg tcgtaggcaa gtgaaaccac cacatcggag ttctgcggga
     2881 atgagcgaat cttttcaaaa ccggatttat cggtaacgag gtttcccagg ttgaaaacag
     2941 cggtgggtgg aacagtgggt ggcaggatgg gctttaccat atccagtttt tcactgataa
     3001 acagtgaatc ggcattgatc aggtaaccca gactatcttc ggctcttaca gcaccggtgt
     3061 ggaagacagc atcactcaca tccacgtcct ttgccttgct tacggcattg gtactatcgt
     3121 agtagtaatt ggtattgcag cgcatgaact ccatccgccc gtacactttc cgcatcttga
     3181 acacccaggt ctcgcggatc atattctgat taaggaagag caaggcaggt ccacccatgg
     3241 aaaaactctg gtagatgaac tccttgttga gctggtcttt ccgaatatac atggtagccg
     3301 aaccgctgac agtatcctgg taaaaagtaa aaagaccttc tgttttctgt ttattttttg
     3361 tctgtgcttt ccaggtggag tcacgcagcg ctttcaccga gtcgataaca ggtttggtct
     3421 taacgggagt aaccggtttt tttacctgag cgagtacaca ttgggcaaat agaaggaggc
     3481 aaaaggccag cagggttggt ttcattcaat gatagtttaa tggtggtgat gaaatacaat
     3541 ttagggtatt tactgcatac cggaaaaatt ttgccgccat tcctgtctac ctcaccccct
     3601 gccccctctc cctgcctacc ggacaggcag gcttgaggag agggggtagc gaggtcgttt
     3661 aaagcagctt caaatcaaac agtatttatt tgcgccaaca gtatctgctt agcattttac
     3721 tgctttaaag tatgttgctc cccctctcct caagcctgcc tgtccggtag gcagggagag
     3781 gggtgagtga attttcaact ttaataaact ttcgaggggt gaggttgtac ggggtgaggc
     3841 attacttcaa cttctttccc gaccttcgca taatcaaatc ctgatttatg aaaaggttat
     3901 ggttctcctt gttcctgata ttagtttaca acacattacc cgcccaacca tcttccctcg
     3961 tcaatccctt tatcggtacc ggcggccacg gccatacttt tcccggggcc acgatgcctt
     4021 tcgggatgat gcaactgagt cctgatacgc ggctcgaagg atgggatggc tgcgggggtt
     4081 atcattactc cgacagtgcg atctatggat tttcgcatac gcatctgagt gggactggca
     4141 ttgccgatta ttgtgatgta ttgctgatgc cgtttaccgg cgaggtgaag tggaagaaca
     4201 aagagtatgc ctctcctttt tcccataaaa atgaaaaagc cagtccgggt tattatgaag
     4261 tgctgctgga taaatttaat atccgggcgg ccctgactac ctccctatgt tcaggtatgc
     4321 accaatacac ttatgatgct tcagctgccg agggcaaagt attactcgat ctgcagcacc
     4381 gtgatgaagt gctgggttcc tccctggaac tggtcaatga atatgaagta aaaggatcca
     4441 ggatcagtaa atcatgggcc aaagaacagc atttgttctt ttatatccgt tttgaaaaac
     4501 cgatcaaacg gtaccgcatg gccctcgatg accagcctgt caatggattg aataaactcg
     4561 aggggaaaaa catcaaatct tatttctctt ttgatcttgg cgaggaaaag atcgtgcggt
     4621 taaggatcgg catctccggc gtgagtacgg agaatgcgaa gcagaacctg gagcaggaga
     4681 tcagtcactg ggattttaac cggttgaaac aacaggcgga gaatgcctgg aacaatgaac
     4741 ttggtaagat tgaagtgaag ggcggaacgc atgatcagca ggtggcattt tatacggccc
     4801 tctatcatac catgattgcc ccgaatatct atacagatgt gaatggagaa taccggggca
     4861 cggatctcaa agtgcacaaa gcagatggct ttaccaatta ctccgtgttc tccctctggg
     4921 atacacatag agctcagcat ccattacaga ccattattaa taagcaacgc acgaatgact
     4981 ggatcaaaac ctttctggcc caatacaagg ccggaggtat gttacccgta tgggaactga
     5041 gtggcaatga aactttttgt atgatcggct accatgccgt accggtaatt gtggatgcct
     5101 ggaaaaaagg cgtgcagtgg tttgataagg aactggcctt aaaagccatg atcgattatg
     5161 cagagagtag ccgatttggc ctccctgctt atatcaaaca aggttatgta agcaatgaag
     5221 atgatcatga gagtacctcc aagacggtgg aatatgcgta tgatgactgg tgcatctccg
     5281 agtttgctgc ccagatcggc aacacgaaga tcagtgagca ataccgcaag cgggcattga
     5341 actaccggaa tttatttgat ccccgcgtaa aacatatccg cggtaaagtt cagggcttct
     5401 ggtattcgcc ctttgatgct acggaagtca ataacttttt taccgagggc aacagctggc
     5461 attacagttt taccgcacaa caggatattg acggattgaa aaaactctat ggcggtcagg
     5521 ccggctttgc gaagcaattg aacgaactat ttac
//
LOCUS       DAOPMW010000218         5505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17145_length_5505_cov_10.648073, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000218 DAOPMW010000000
VERSION     DAOPMW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_5505_cov_10.648073"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..808)
                     /gene="recO"
                     /locus_tag="PLU11_10410"
     CDS             complement(62..808)
                     /gene="recO"
                     /locus_tag="PLU11_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPN59580.1"
                     /translation="MADKLHKTKGLVLRTVKYGETSIIVTIFTELFGVQTYLVNGVRS
                     SSKKGAGKAGLFQPAALLDLVVYHNELKQLNRIKEFRWSRLYQHIFSDVPKNAVALFM
                     VELLTKCLRQPEGNPDLFQFTEDCFLHLDQSQGAVMANLPLFFALHLPVHFGFRMTDN
                     HSEEEVYLDLKEGQFSAEHPGHPYFLEGQQAVTTSQLLKVMQPEELDQIRLHHDFRRL
                     LLHQLELYYALHIQDFGTMKTLPVLREVLG"
     gene            complement(808..3090)
                     /locus_tag="PLU11_10415"
     CDS             complement(808..3090)
                     /locus_tag="PLU11_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN59581.1"
                     /translation="MRTRFILCFLLAGLFTAAQDQLAPVGQWREHLPYNSAIDLAAGN
                     TRIFCATPYSLFSVTYTDAQTERFSRVTGLSETGISTICLNNNRDKLLVAYTNSNLDI
                     LQLNRVYNIPDIKRDNIPGDKSIYRIYALQDKFYLASGLGVIVVDANRYEISDTWLVG
                     NGGNPVKVTGFSSDAGFYYAATTEGLKKAAIGITDPADYRNWQTIPGTLGVQQVLNLS
                     GTMVLQKNDSLFIQNGNNWNLFYQDGWPLVNVEVSENKLLLCERQTTGISRVVILDPG
                     GAVNRVLDNTGAVSFPRKALLVNNEPWVADQFAGLSRIANSNAYANFSPGSPQAVGGG
                     EMAVADKVFYATAGAVNDAWNYQYNGDGIFILKEGSWTNINRYRYPAIDTLLDYIALA
                     YDKRDASIWAGSYGGGLLHLNEGPAFTIYKQGYLDVTAGDPGSYRVAGLCFDTEQNLW
                     VSNFGAPQPLRVMKADGSWKSFSIPFTLFENAVAQIIVDDNGYKWIVSPLGNGLIVFD
                     HGNSIDNTGDDRWRKLGMNAGNGNLPSSNVTCLAKDKSGFIWVGTTDGVAVFQCPDQL
                     FSTGCDAYWPIVPNGNFAGYLFKGEEVRSIAVDGADRKWVATKNGVFLVDASGEKVLA
                     RFTETNSPLLSNEVNKMAIDGKTGEVFFATLKGICSFSGNATEGGITNEEVLVFPNPV
                     PPGFGGTIAIRGLVNNAIVKITELDGKLVYQTRALGGQANWDGKNYRGQRIASGVYLV
                     LVQEDGGKERTATKIFFINR"
     gene            3172..>3413
                     /locus_tag="PLU11_10420"
     CDS             3172..>3413
                     /locus_tag="PLU11_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HPN59582.1"
                     /translation="MWYRLGQFILQYRIALLVLLLVLTGFMGYHASKVQLSYDFTRAI
                     PLDNPKYKAYQEFRKKFGDDGNLLVIGIQTDQFFNE"
     assembly_gap    3414..3513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3514..5406
                     /locus_tag="PLU11_10425"
     CDS             <3514..5406
                     /locus_tag="PLU11_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN59583.1"
                     /translation="NKDLMASKAREGVVKEINKVTDAFGEVQNLTIYKSGLPYIRTEI
                     AIMLQEEMRFFLIASIALSALILLLFFRSISATILSLLVVGIGVVWSMGTIELLGYRI
                     SLLTALIPPLVVVIGIPNCIYFLNKFHVSWNQSGDKRQSLVAMVDKMGIVTLFCNVAA
                     AIGFAVFALTQSQILKEFGVVAGINIMALFLISLILIPAALSFLPAPKTRHTKYLDNP
                     RLNRWLDRLERWSLNHRRLIYGITTALVIVSLFGIYRLKSEGFIVDDLPKSDKIYTDL
                     KFFEKNFKGIMPLEVVVDTKKKQGVVRNPANLEKIDTFSRFLANIPDIARPLSLAEGL
                     KFAKQAFFDGDPANYNMPSSNDLVALSQYLSFKGDTTGSKSSLATLVNSFMDSTKQRA
                     RISVNMADIGSARLPGMLDSIQEMAEKIFNTDSVEALTADTATREANKEDSTWGKRRF
                     DIQLTGTSVTFLEGSRFIISGLKDSIFWAFLLIAACMLYLFRSFRILICSLIPNIIPL
                     MITAGVMGWVGVPLKPSTVLVFSVALGIAIDVTIRFLVNYKQELPAQNYDLTKTVIET
                     IHSTGISIIYTSLVLIAGFVIFYFSEFGGTKALGWLTSLTLVTATLTNLVLLPAILIS
                     FIRVKK"
BASE COUNT         1379 a         1406 c         1184 g         1436 t
ORIGIN      
        1 actcattact catttcttct ttaaacctct acccatacta taagaactct tcccgacaca
       61 atcacccaag cacttccctt aacaccggca acgtcttcat cgtcccgaag tcctgtatat
      121 gcaacgcata atacaattcc agctggtgaa gtaacaatcg tctgaaatca tgatgcagcc
      181 ggatctggtc cagttcttcc ggttgcatga cttttaataa ttgtgaagta gtgactgcct
      241 gctgaccttc caggaaatac ggatgccccg gatgttcggc tgaaaactgt ccctctttta
      301 aatccaggta cacttcttcc tcactgtggt tatccgtcat ccggaaacca aaatgtaccg
      361 gcaggtgcaa ggcaaaaaat aaaggcagat tcgccattac cgcaccctga ctttgatcca
      421 ggtgcagaaa acaatcttct gtaaactgga aaagatccgg attgccttcg ggttgccgca
      481 ggcatttggt cagcagttcc accataaaca aggccacggc atttttgggc acatccgaaa
      541 aaatatgctg ataaagccgg gaccagcgga actcttttat gcggttgagc tgtttcagtt
      601 cattgtggta caccaccaga tcaagcaggg cagccggttg aaataaaccc gctttaccag
      661 cgcctttttt agaagaactc cgtacgccat tgaccaggta agtctgtact ccgaaaagtt
      721 cggtgaagat ggtcacgatg atactggtct ccccgtactt caccgtacgc agcaccagcc
      781 cctttgtttt atgcagttta tcggccatca gcgattgata aaaaatattt ttgtagctgt
      841 ccgctctttc cctccatcct cctgtaccag caccaggtaa accccgctgg ctatccgttg
      901 accccggtaa ttcttcccat cccagttggc ctgtccgcca agcgctcttg tctgatacac
      961 taacttccca tccagttccg tgattttgac aatggcatta ttcactaatc cacggatggc
     1021 aatagtacca ccgaatcctg gaggaaccgg gttaggaaac accaatactt cttcattggt
     1081 tattcctcct tccgtcgcat ttccgctgaa tgaacagatt cctttcaatg tggcgaaaaa
     1141 gacttccccc gtttttccat cgatcgccat tttattcact tcattactga gcaagggact
     1201 gttggtttcg gtaaaacggg ccagtacttt ctctcccgag gcatccacca ggaatacccc
     1261 gttttttgtg gccacccatt tccggtcagc cccatccacc gctatacttc gtacttcctc
     1321 gcctttaaaa agatagccgg caaaattgcc attgggtaca atgggccagt aagcatcaca
     1381 gcccgtagaa aataattggt caggacattg aaataccgcc accccatcgg tagtacccac
     1441 ccagataaat ccgctcttat cctttgccag gcaggttaca ttggaagaag gcagatttcc
     1501 attaccggca ttcataccca gcttccgcca gcggtcatct ccggtattat ctattgaatt
     1561 accatgatca aatacgatca atccgtttcc taaaggagat acgatccatt tatacccatt
     1621 atcatccacg ataatctgtg ctactgcatt ttcaaaaagc gtaaatggaa tggaaaaact
     1681 tttccagctg ccatccgctt tcattacccg caatggttgc ggagccccga aatttgatac
     1741 ccacaaattt tgttccgtat caaaacagag accggccacg cggtaactgc ccggatcacc
     1801 ggccgtgaca tcgagatagc cctgtttgta aatagtaaat gcgggacctt cgttcaggtg
     1861 tagtaatcct cccccatacg aaccggccca gatactggca tcccgtttat cataagccag
     1921 ggcgatataa tccagcagcg tatcaatggc cggataacga tagcggttga tattggtcca
     1981 gctgccttct tttagtataa aaataccatc cccgttatac tggtaattcc aggcatcatt
     2041 aaccgcccct gctgttgcat aaaaaacctt gtccgccacc gccatttcgc cgccgccaac
     2101 agcctgcggg gagcccgggc taaaattcgc ataggcattg ctgttagcaa tccgggaaag
     2161 tccggcaaac tgatccgcca cccagggttc gttattgact aaaagcgctt ttcgggggaa
     2221 agacacggcc ccggtattat ccagtacccg gttaactgca ccgccgggat cgaggatcac
     2281 cacccggctg atcccggtcg tttgtcgttc acaaagcaac agtttgtttt cactgacttc
     2341 cacattcacc aatggccagc catcctggta aaacaggttc cagttatttc cattttgtat
     2401 aaacagggag tcgtttttct gtaaaaccat agtgccggaa agattcaaaa cctgctgaac
     2461 acccaatgta cctggtattg tttgccagtt ccggtaatca gccggatcgg ttattccgat
     2521 tgcggctttt ttcaagcctt ctgtcgtggc cgcataataa aaaccagcat cgctggagaa
     2581 accggtgact ttaaccggat tgcccccgtt tccaaccaac caggtatcgc tgatctcata
     2641 ccggtttgca tcgaccacga tcacaccgag tccggaagcg aggtaaaatt tatcctgcaa
     2701 tgcataaatg cgatagatcg atttatctcc cggtatatta tcccgcttga tatccggaat
     2761 attataaacc cggttgagct ggaggatatc caggttgctg ttcgtataag ccaccaggag
     2821 tttatcccgg ttattattca ggcagatcgt actgatccct gtttcactca gtccggtaac
     2881 ccggctgaac cgctcggttt gtgcatcagt gtaagtgact gaaaaaaggc tgtaaggagt
     2941 ggcacagaaa atacgcgtat taccggctgc caggtcaatg gcactgttat acggcaggtg
     3001 ttcccgccat tgccccacag gtgccagctg atcctgtgcg gctgtaaaca gtccggccag
     3061 taaaaaacaa aggatgaaac gggttctcac ccctcaaaag tatcacaatc agtcatgttc
     3121 ctattgggct tttttgcctt tctttgcagc ctcacaatcc ctgaaacatt tatgtggtac
     3181 cggctcggac aatttattct ccaatatcgt attgcactcc tggttctttt actggtcctg
     3241 accggtttta tgggctatca tgccagcaaa gtccagttaa gttatgattt tacccgcgcc
     3301 atcccgctcg ataatcccaa atacaaagcc taccaggaat tcaggaaaaa attcggcgac
     3361 gatggtaacc tgctggtgat cgggatacag acggatcagt tttttaatga aaannnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaataaag acctgatggc ttccaaagca
     3541 agggaaggcg tggtaaaaga aataaacaaa gtgaccgatg cttttggtga ggtgcaaaac
     3601 ctgaccatct ataaaagcgg gcttccatat atccggacag aaatcgcaat tatgctgcag
     3661 gaagaaatgc gttttttcct gattgcgtcg attgccttat ccgcccttat cctgcttttg
     3721 tttttccgct ccatcagtgc caccatactt tccctgctgg ttgttggtat cggggtggtc
     3781 tggagtatgg gaaccattga actgctcggg taccggatca gcctgctcac tgcattgatc
     3841 cccccgctgg tggtggtaat cgggataccc aactgtatat attttctcaa taaattccat
     3901 gtgtcctgga accagtccgg agataaacgg caatccctgg tggcaatggt ggacaaaatg
     3961 ggaatcgtga ccctgttttg caatgtagcg gcggccatcg gctttgccgt atttgccctg
     4021 acccagagcc agatcctcaa agaattcggg gtggtcgcag gtattaatat catggctttg
     4081 tttctgattt cactgatcct gattccggcc gcattaagtt ttttacccgc cccgaaaacc
     4141 aggcatacca aatacctcga taatccccgg ctaaaccgct ggctggatcg tctcgaaaga
     4201 tggtccctga accaccgccg cctgatttat ggcattacca cggccctcgt catagtttcg
     4261 ttgtttggca tttaccgcct gaaaagcgaa ggtttcattg tggatgatct gccaaaatcg
     4321 gataaaatct atacggacct gaagtttttt gaaaagaatt tcaaaggcat catgcccctg
     4381 gaagtagtgg tggataccaa aaagaaacag ggagtggtcc gcaatccggc taacctggaa
     4441 aaaattgata cattctcccg tttcctggcc aatattcctg atatcgcccg gccattgagc
     4501 ctggccgaag gtttaaaatt tgccaaacag gccttcttcg atggagatcc ggccaactat
     4561 aacatgccat ccagtaatga cttggtggcc ctgtcccaat acctcagttt taaaggagat
     4621 acaaccggca gtaaaagttc gctggccacc ctggtgaaca gttttatgga cagcaccaaa
     4681 cagcgggccc ggatcagtgt gaatatggcc gatatcggca gtgcccggtt acccggcatg
     4741 cttgacagta tccaggagat ggccgaaaaa atctttaata cagacagtgt ggaagccctg
     4801 accgcagata cagcaacccg ggaagccaat aaagaggatt ccacctgggg aaagcgccgg
     4861 ttcgatatcc agctgaccgg cacaagtgta accttcctgg aaggtagccg gtttattatc
     4921 agcgggttaa aagacagtat tttctgggcc ttcctgctga ttgccgcctg tatgctgtat
     4981 ttattccgat ccttccggat cctcatctgt tccctgatcc cgaacatcat tccgctaatg
     5041 atcaccgccg gggtgatggg ctgggtgggc gtaccactga agccctccac cgtcttggtt
     5101 ttcagtgtgg ccttaggcat tgcaatcgac gtaactatca gatttttagt taattataag
     5161 caagaattgc ccgcacaaaa ctacgatctg accaagaccg taattgaaac catccatagt
     5221 acaggtatca gtattatcta cacatcattg gtactgattg ccggttttgt aatcttttat
     5281 ttcagtgagt ttgggggtac caaggcattg ggctggttaa cctctctgac cctggtcaca
     5341 gctaccctca ccaacctggt acttttgccg gccattctta tttcttttat tcgggttaag
     5401 aaataggcga tttcattact tttacggcag gactgcaggc ttttaatgcc ctgtaagtgc
     5461 agcaattaat ttaggttaat tgtcaaagag ttggcgggag aatgg
//
LOCUS       DAOPMW010000219         5499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17174_length_5499_cov_5.952241, whole genome shotgun sequence.
ACCESSION   DAOPMW010000219 DAOPMW010000000
VERSION     DAOPMW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17174_length_5499_cov_5.952241"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLU11_10430"
     CDS             <1..248
                     /locus_tag="PLU11_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59584.1"
                     /translation="FSEYDSDNLLLRQAYQEVLDQQMEEARLRDMLQRIQESTIVLTF
                     PVQLTPFCFPIKVDSMREDLSSEKLEDRIKKMQQQLK"
     gene            280..948
                     /gene="pdeM"
                     /locus_tag="PLU11_10435"
     CDS             280..948
                     /gene="pdeM"
                     /locus_tag="PLU11_10435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HPN59585.1"
                     /translation="MKNPIPHILREQTLWLSPERCILWEEENTLIVADLHLGKSGHFR
                     KEGIGIPQSIYKADLQRLMAQLYLYKADRLIIAGDFTHSTANKELDLFLKWRKDFSLL
                     QIDLVKGNHDILGDQWYETAGIQVHEWKLAAGPFLFLHDKKAEKDLTAEELSMYRFTG
                     HLHPAITLKGKGKQTLRFPCFYFTRDCAVLPAFSRFTGGFKVEPVKGEQVFALVEDGI
                     MQLS"
     gene            complement(945..3398)
                     /locus_tag="PLU11_10440"
     CDS             complement(945..3398)
                     /locus_tag="PLU11_10440"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN59586.1"
                     /translation="MRLRYYYYIALLLLCFQSVAQVKTTQAVKTLQAPRIDGVLNDAV
                     WQDAPVLTDFIQNSPSPGSKSSSGTEVRILYDNSAIYVGAYLFDDPALIRKQFTARDG
                     EQMRDVDYFSVFFDTYNDKQNGFQFLVTTANVQSDARVSPTYSGEFGSYGDKTWDAVW
                     ESKVSIQKNGWVVEMRIPYVSLRFSKKEVQDWGLQLLRFTRRNNETDFWNPISPQVNG
                     FANQFGQLTSLQHIEPPLRLSFSPYVTTGFRSSPIGNSYLNEWLRNGGMDLKYGLNES
                     FTLDATLVPDFGQVISDNVVNNLTPYEVQFQENRQFFTEGTEIFNKAKLFYSRRVGAL
                     PGGYYSVLGMADADPDLEIIKNPTSTQLYNAVKFSGRTTKKLGIGIFNAVTAPMKAVL
                     RNKISGLETTIETEPLANYNILVLDQAFKGRSSLTFTNTNVMRNGAARDANVSAVDFA
                     LYDSRNLHALQGTARYSKIWSANGYDGYNTTLRYGKVSGNWQYYVLGNVESKNYDPND
                     LGILQAANEISYRASISYRRYKPTRSLIDYSYSLLPRLQYLYQPYAYNRFDVQARGFW
                     LFRNFWDLTVVAGVVPGWENNYFELRTDGRPLRYPSNYSLEASGSTDSRKKAFVSFGL
                     VYAVAPEFSNTFRSVSLGMRYRFGDRFSLDLQTSSHLEDNQLGYAFMRETNGEPIVGF
                     RKNRDFTSVLSGIYNFAYRLNLTLRARHYWNKVNYYSFHNVDMKGDLISRAFISNQDD
                     NVNVFNVDAFLTWDFRLGSRLILGYKNWLGNEENIQFGSGQNSYLRNLGKTFDLRHGN
                     EVTVRFIYFLDYNQLRRKP"
     gene            complement(3493..4242)
                     /locus_tag="PLU11_10445"
     CDS             complement(3493..4242)
                     /locus_tag="PLU11_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HPN59587.1"
                     /translation="MAVSNQNLKYLRKLRGWTQEEFANKLHIKRSLLGAYEEERAEPR
                     IDVLEVVCDMFKLTLDEILRKDLSDNKANYLAKRRAMKLAAGKSEIQFVPVKAAAGYL
                     AGYADPEFIDELNTFTLPMLTGGNYRAFEIIGDSMLPTPSGSVIVGEKVDNFEDMKNN
                     AACIVISRNEGIVYKRVQKHNKLKNKLTLISDNPSFHPYTVNAEEVLEMWQAQVVIAR
                     ANTQNRWDMNQLANVVSDLQQQVSSLKKRIN"
     gene            4350..5159
                     /gene="surE"
                     /locus_tag="PLU11_10450"
     CDS             4350..5159
                     /gene="surE"
                     /locus_tag="PLU11_10450"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPN59588.1"
                     /translation="MKKKAITSPKKKSRVNEKPVILITNDDGITAPGIRSLVEAVKDI
                     GKIVVVAPDKAQSGMGHAITIGQPLRLHKVQMFEGVESWSCTGTPVDCVKLAVDKVLH
                     RKPDLCLSGINHGANHSINVIYSGTMSAAVEAAIESIPSAGFSLLDYSIDADFSGAKK
                     YARIIVEKMLSTKLEKHTVLNVNVPSKPEILLKGIKICRQAYAKYVEDFIERKDPHGK
                     HYYWLTGEFVNFDKGKDTDVWALANNYVSVVPVQFDLTHYAMKDKLKNWEK"
     gene            5156..5452
                     /locus_tag="PLU11_10455"
     CDS             5156..5452
                     /locus_tag="PLU11_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59589.1"
                     /translation="MIFTKDNLKLGLVLGLVGPLAGLAVVYFVSYSAFTFIEFLDNFI
                     HENKLITSIGSMSLLANAVLFTLYVNTNRDKTAKGIFIVTLIYGIGILVLKIFN"
BASE COUNT         1505 a         1264 c         1265 g         1465 t
ORIGIN      
        1 tattcagtga atatgattcg gacaacctgc tgctgcgcca ggcctaccag gaagtactgg
       61 atcagcaaat ggaagaagcc cggttgcggg atatgcttca acggatccag gaatcaacaa
      121 tcgtccttac ttttccagta cagctcaccc ccttctgttt cccgattaaa gtggatagta
      181 tgcgggaaga cctttcatca gaaaaactgg aagaccggat caaaaaaatg caacagcaac
      241 tgaaataagc caattgtttt atagatttga cccctgtcaa tgaaaaaccc gattccccat
      301 attctccgcg agcaaacact ctggttatcc cccgaacgtt gtattttatg ggaagaagaa
      361 aacaccctga ttgtggccga tcttcacctg ggtaaatcgg ggcatttccg gaaagaagga
      421 attggtattc cccagagtat ctataaagcg gatctgcaac ggctgatggc acagctttat
      481 ctgtataagg cggacaggct gattattgct ggtgacttta cccatagtac ggctaacaag
      541 gaactggatc tctttctcaa atggcgaaag gatttctccc tgttgcagat cgacctggtg
      601 aaagggaatc atgatatcct gggtgatcag tggtatgaga ccgccggtat tcaggtacac
      661 gagtggaaac tggctgcagg cccattttta tttctacatg ataagaaagc ggaaaaagac
      721 ctgaccgctg aagaactgag catgtaccgg tttaccggtc acctgcatcc ggccattacc
      781 ttaaaaggga aaggcaaaca aacccttcgt ttcccttgct tttattttac cagagattgt
      841 gcggtactac cagcgtttag ccgtttcacg ggtggattta aagttgagcc cgtaaaaggg
      901 gaacaggttt ttgccctggt ggaagacggc attatgcaac tgtcttaagg tttgcggcga
      961 agctggttgt aatccagaaa atatataaag cggaccgtca cttcattacc atgacggaga
     1021 tcaaatgttt tgcccaggtt acgcaggtaa ctgttctgac cggaaccaaa ctgaatattt
     1081 tcttcattcc ccaaccagtt tttatatcca aggatcagcc ggcttccgag acggaaatcc
     1141 caggtcagga aagcatccac attaaataca ttgacattgt catcctggtt gctgataaag
     1201 gcacggctga tcagatcgcc cttcatgtct acattatgaa aactataata gtttacttta
     1261 ttccagtaat gccttgccct gagtgtcagg ttgagccggt aggcaaaatt atatatacca
     1321 gaaaggacag aagtgaagtc gcggtttttg cggaatccca cgatcggttc cccgtttgtt
     1381 tcccgcataa acgcgtagcc taattgattg tcttcgaggt gtgagctggt ttgaagatcc
     1441 agtgagaatc gatccccgaa gcgataacgc atgccgagtg aaacactccg gaatgtatta
     1501 ctgaactccg gtgctacagc atataccaat ccgaaactga caaaggcctt cttccggcta
     1561 tcggtactgc ccgaggcttc cagggaataa ttggacggat aacgaagggg acgtccgtct
     1621 gtacggagtt cgaaatagtt gttttcccaa ccgggtacca cgccggcaac cacggtcagg
     1681 tcccagaaat tccggaataa ccagaagccc ctggcctgca catcaaaccg gttataggca
     1741 taaggctgat acaggtattg caaacggggc aacaaggaat aactgtaatc aataagcgaa
     1801 cgggtgggtt tgtaccggcg ataagaaata ctggcccggt aactgatttc attcgctgcc
     1861 tgcagaatgc cgagatcatt cgggtcataa ttttttgact ccacattccc gagtacatag
     1921 tattgccagt tgccactgac tttcccatat cgcagggtgg tgttataccc atcatagccg
     1981 ttggccgacc agattttact gtaccgggct gttccctgta aggcatgcag gtttcgtgaa
     2041 tcataaagtg caaaatcgac agcagataca ttggcatcac gggccgcacc attgcgcatt
     2101 acgtttgtat tggtaaaggt cagggaggag cgacctttaa atgcctggtc cagtaccagg
     2161 atattgtaat tcgccagcgg ctctgtttcg atagtggtct ccagaccact gatcttatta
     2221 cggagaactg ctttcattgg agccgttacc gcgttaaata tcccgatacc cagttttttc
     2281 gtagtgcggc cggagaattt aacggcatta tataattggg tgctggttgg attcttaatg
     2341 atctccaggt ccgggtcggc atcagccatc cctaataccg agtaataacc accggggagt
     2401 gctcctaccc tgcgtgaata aaagagtttt gccttattga aaatttctgt gccttcggta
     2461 aaaaactgcc ggttttcctg aaactgcact tcataaggag tcaggttatt gaccacattg
     2521 tcagagatca cctgtccaaa atccggtacc agggtggcat ccagtgtaaa actttcattt
     2581 aacccgtatt tgagatccat tcctccattc cggagccatt cattaagata actgttccca
     2641 atgggcgaag aacggaatcc tgttgtaaca taaggggaaa aactaagccg caacggtggt
     2701 tcaatatgct gtaagctggt cagctgccca aactggttgg caaaaccatt cacctgcggg
     2761 ctgatcggat tccagaaatc cgtttcatta ttccggcgtg taaagcgtag tagctgcagc
     2821 ccccagtcct gtacttcctt ttttgaaaac cttagggaga catatggaat acgcatttca
     2881 accacccatc cgttcttttg gatactcact ttactttccc aaacagcatc ccaggtttta
     2941 tcgccatagc tgccaaactc tcctgagtaa gtgggcgata cccgggcatc cgactgtaca
     3001 ttggccgtgg taaccaggaa ctggaaacca ttttgtttgt cattatacgt atcaaaaaaa
     3061 acagagaaat agtccacatc cctcatttgc tcaccatccc tggcggtaaa ctgctttcgg
     3121 attagggctg gatcgtcaaa gagataagcg ccaacatata tagctgaatt atcatacaaa
     3181 atccggactt ctgtgccgga gctgctttta ctaccggggg agggagaatt ctgaataaaa
     3241 tcagttaata caggtgcgtc ctgccatacg gcatcattca acaccccatc aatgcggggt
     3301 gcttgtaagg tttttacagc ctgtgttgtt ttaacctgcg caactgattg aaaacaaagg
     3361 agtaaaaggg caatatagta ataatatctc agcctcatct gcagtagtcc tgctacaaat
     3421 atatagacaa aaaagcagga ccaaaggctg cccttacatt ggttgaaaac ttactaagtg
     3481 attgtttgat actcagttaa tacgtttctt tagggaactt acctgctgtt gcagatcgga
     3541 taccacattg gccagctggt tcatatccca gcggttctgg gtatttgccc gggcgatgac
     3601 cacctgggcc tgccacattt ccagcacttc ctccgcattt accgtatacg gatgaaaaga
     3661 tgggttatcg ctgatcaggg tcagtttatt tttaagctta ttatgcttct gtacgcgttt
     3721 ataaacaatg ccttcattcc tggaaatcac aatacaggcg gcattgttct tcatgtcttc
     3781 aaagttgtcc actttttcac cgacaattac cgatccgctg ggggtaggca gcatcgagtc
     3841 cccgattatt tcaaatgccc ggtaatttcc cccggttaac atcggaagcg taaaggtatt
     3901 cagctcatca ataaattccg gatccgcata accagccagg tagccggcgg ctgcttttac
     3961 cgggacaaac tggatttctg attttccggc agccaacttc atggctcttc gcttggcgag
     4021 ataatttgcc ttattatcgc tcaggtcctt ccgtagaatt tcatccaatg ttagcttaaa
     4081 catatcacaa acaacttcca ggacatcaat acggggctcg gccctttctt cttcataggc
     4141 acccagcaag gaacgtttaa tgtgtagttt gttggcaaac tcctcctgtg tccagccacg
     4201 cagcttgcgg agatatttca ggttttgatt tgagacagcc atgatagtct aatttattta
     4261 gctgcaaaat actaattatt ttagatattc caaatccttt cttttaagcg gcttggataa
     4321 cccggaaaaa acaaggattt ttgtatccta tgaaaaagaa agccataacc agtcctaaga
     4381 aaaaaagccg ggtaaatgaa aagccggtga tcctgattac caatgacgac ggcattactg
     4441 cacccggcat ccgcagcctg gtggaggccg taaaggatat cgggaaaatt gttgtagtgg
     4501 cccctgataa agcccagtcg ggcatgggcc atgccatcac cattggccag ccgctgcgcc
     4561 tgcataaagt gcagatgttt gagggagtgg aatcctggtc ctgtaccgga acgccggtgg
     4621 attgtgtgaa actggccgtg gacaaagtat tgcaccgtaa accggatctc tgtctgagtg
     4681 gcatcaatca tggcgccaac cacagtatca atgtaattta ttcagggacg atgtcggccg
     4741 cggtggaagc cgccattgaa agtattccat ctgccgggtt ctccctgctc gattacagta
     4801 ttgacgcgga ttttagcgga gctaaaaaat atgcccgtat cattgtggag aaaatgctga
     4861 gtaccaaact ggagaaacat acggtgctga atgtaaatgt gccttctaaa ccggaaatac
     4921 tcctcaaagg cattaaaata tgccggcagg cctacgcaaa atatgttgaa gattttattg
     4981 aacggaaaga cccgcatggc aagcattact attggctgac cggtgagttt gtgaattttg
     5041 ataaaggaaa agataccgat gtgtgggccc tggcaaataa ttatgtgagt gtggtaccgg
     5101 tacagtttga cctgacacac tatgccatga aagacaaact taaaaactgg gaaaaatgat
     5161 attcaccaaa gacaacctga aacttggtct ggtgcttgga ttggttggtc cgctggccgg
     5221 actggcggtg gtttattttg taagctattc agcttttacc tttattgaat tcctcgataa
     5281 ttttatacat gagaacaaac tcatcacctc cattggttcc atgagtttgc tggccaatgc
     5341 ggtactattc acgctctatg taaataccaa ccgggataaa acggctaaag gaatatttat
     5401 tgtcaccctg atttacggta tcggcatcct ggtattaaaa atcttcaact aaggggttca
     5461 tgaagtatta catcatagca ggggaagcct ccggtgatt
//
LOCUS       DAOPMW010000220         5494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17200_length_5494_cov_3.718514, whole genome shotgun sequence.
ACCESSION   DAOPMW010000220 DAOPMW010000000
VERSION     DAOPMW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17200_length_5494_cov_3.718514"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1785)
                     /locus_tag="PLU11_10460"
     CDS             complement(100..1785)
                     /locus_tag="PLU11_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPN59590.1"
                     /translation="MYKSSLYVLTLCLLNGFAQSLSAQGEPFTSRTVVSGLNSAWEVL
                     YGPNDSLWVTENRSYLISRISIANGTKTVLVDLRATDGTINFLASSPNQPQGGLMGIA
                     IHPNLYSTDAATRAAKPWVYAAYVYNKGVCPGSNTSCIYTTKIVRFDYDGNTLTNPTT
                     IINTIPGSSDHNSGRLVISPVIEPGADAAHTQYRLYYTVGDMGAGQFLNTTRAENAQV
                     LNNLEGKVLRLNTESDGDAGQDAWIPNDNPFFNASSITAQDYVFTLGHRNAQGLAWGN
                     VNGTNILYSTEQMDRTDDEINIITAGKNYGWDQVSGYCDDNVNGYKIGQNTSANENAF
                     CSVPANNHQEPIFTTFTEPASGMAALMAQSNNQLWPTIASSSVDFYSSGKISGWKYSL
                     FISPLKKDRVYRVKLNADGSDVVGDTIPYFAGNGNRIRRIRVSPDGLKFYVARDVGAT
                     SNAGAIMEYTYAGTVLSLNENPAEGPARVNDMISIYPNPVTDILNIHGKRDLRKPLFA
                     QIMDINGRLLLTGSSFRNDFTMDVQALQAGTYFLKLYNGNNMEVQVVKFVKVK"
     gene            1965..4259
                     /locus_tag="PLU11_10465"
     CDS             1965..4259
                     /locus_tag="PLU11_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59591.1"
                     /translation="MKTTIPNCIHPYLLVILVALLPGSVQVMAQNIDFGKSYINVTKG
                     LNGGTVETGDTLEIRATSVVRSGTYDSCRYQDAVPASTVYIPGTVRVLTNEGKIYKQF
                     TDTYGDDEGWITGSNVTINLGYRTGAAPASAFRRGRVANTHKPSFYGSSCIMIASFRV
                     RVTAATGSNISTGGGNMTYKLGSASINTFTFPANTLRVYPNYGICSNSIGTNAIGTEF
                     NGTFGSGQPRNRGTSGNVPVGYTYNFFDLNSPQDYYYGIANNTSTRSNYTTSNSWAKP
                     DNSSPTHRVFTVWDIIGDHTGALNPVLGNPAADTVANPNAGYMLVINAAYRIDSAFQQ
                     TISGLCPNTYYEISCWMRNICSKCGCDSNGKGASNSSGPPYYIPTGTGDSSGVAPNLT
                     FEIDGIDYYTSGNLMYSGQWVKKGFTFLTGAAQTSFTLKFFNNAPGGGGNDWALDDIS
                     VSTCSPNMSYSPSLTPIVCDSNILTIYDTVRSFFNNYTYYKWQRSTDGGTNWSDVTAP
                     AGPATPVWNGTAWEYVTSYTVPASETLLTNNGDLYRLVVATTSANLSNALCNFTDVAN
                     IIAMNVIDCGIPLAAQLLSFNGKNNNALAELQWTTSEEEEPLQFIIEKSPDGIHFSEI
                     GSVNSHLNNTGEEQIYNFTDPVALSGKTYYRVRIQNQRGQSIWSRTIQLSNRQEKLSF
                     VSVINPFSHELQLDIAASQYSKSEAELIDASGKIVRHKTVDLASGVTRIKFENTMGLA
                     PGMYFLRIRNQDGYIQKTVLKTNQ"
     gene            complement(4263..4931)
                     /locus_tag="PLU11_10470"
     CDS             complement(4263..4931)
                     /locus_tag="PLU11_10470"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPN59592.1"
                     /translation="MKLRSVALLIALIIIADQALKIWIKTSHDTGEVLRVFGADWFRL
                     HFIENPGMAWGWKFGNETGKMILTLFRLAAVIFGTWYLGQIVKQKYSKGFIICAGLIY
                     AGALGNLIDSMFYGMIFDKGLHYDTALNDYISYSGVAAFSSNGYSSFLHGSVVDMLYF
                     PIIDTNGFVFFSPIFNIADASISIGVITLLIFQKRFFKKPEPADANPTVETSSEISDK
                     MQVS"
     gene            complement(5049..>5494)
                     /locus_tag="PLU11_10475"
     CDS             complement(5049..>5494)
                     /locus_tag="PLU11_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HPN59593.1"
                     /translation="GIGLYGVDSAGTAALDLQTVATLKSTIAQLKWLKPGDSVSYNRK
                     GRVEKASLIATIRLGYADGYPRRLGNGKGSVLINGKLAPVIGTVCMDMFMADVTAIPG
                     VTQGDEVIIFGKELPVTELARWADTIPYEILTGVSQRVKRVYFEE"
BASE COUNT         1491 a         1327 c         1252 g         1424 t
ORIGIN      
        1 tacaggagag tagtgcctgt aatacatact gatctttagt gataactaaa cctatgtgtt
       61 aaaaaaatcc gcgaagcgga tagcggccac gccgcccccc tacttcacct tcacaaactt
      121 caccacctgc acctccatat tattcccatt gtacagtttc aggaaataag tgccggcctg
      181 tagtgcctgt acatccatgg tgaaatcatt gcggaagctg cttccggtaa gcaagaggcg
      241 tccattgata tccatgatct gggcaaagag tggtttgcgt aaatctcttt tcccgtgaat
      301 attcaggata tcggtgaccg gattcggata gatgctgatc atatcattca cccgggctgg
      361 cccttcagcc ggattttcat tgagagataa aaccgtacct gcgtaggtgt attccattat
      421 cgcacccgcg ttactggtgg cgcccacatc acgcgctaca taaaatttta atccgtccgg
      481 agataccctg atccgccgga tacggttccc attcccggca aaatagggaa tcgtatcgcc
      541 gaccacatcg gacccgtccg catttaattt tacccggtaa accctgtctt ttttaagcgg
      601 actgataaag agagaatact tccagccgct gatcttacct gaactgtaaa agtccacact
      661 ggatgaggcg atggtgggcc acaattgatt attcgattga gccatcaacg cggccatgcc
      721 ggatgcgggc tctgtaaagg tggtgaaaat gggttcctgg tgattattgg ctggtaccga
      781 acaaaaagca ttttcattgg cgctggtatt ctgaccgatt ttatacccgt ttacattgtc
      841 atcacaataa ccggaaacct ggtcccatcc ataatttttt ccggctgtaa taatattgat
      901 ctcatcatcg gttcggtcca tttgttccgt gctgtacaaa atatttgttc cattgacatt
      961 accccaggcc agtccttgtg cattccggtg ccccagtgta aatacataat cctgtgcagt
     1021 aatagacgat gcattgaaaa aaggattgtc attgggaatc caggcatcct gtcctgcatc
     1081 gccatcgctt tctgtattga ggcgtaacac tttcccttcc aggttattca gcacctgtgc
     1141 attttctgca cgagtggtat ttaaaaactg accagcaccc atatccccta cagtataata
     1201 gagacggtac tgagtatgtg cggcatcggc ccctggttct atgaccggac tgatgaccag
     1261 cctgccggaa ttatgatcac tgctgccggg aatcgtattg ataatagtgg tgggattggt
     1321 aagtgtattc ccatcataat caaagcgtac aatctttgtg gtgtaaatac aactggtatt
     1381 gctacccgga caaacccctt tattatatac ataggctgca tagacccagg gtttggcagc
     1441 gcgggttgcc gcatcagtgg aataaagatt cgggtgtatg gcgattccca ttaatccacc
     1501 ctgcggctga ttcggagagc tggctaaaaa attgattgtg ccgtctgtgg ccctcagatc
     1561 caccaaaacg gtcttagtcc cgttggcaat actgatgcgg gaaatgaggt aagacctgtt
     1621 ctctgtcacc cagagtgaat cattgggtcc atagagtact tcccaggctg agttcagacc
     1681 ggaaaccacc gttcgggaag taaatggttc accctgagca gacaaggatt gggcaaagcc
     1741 atttaacaaa cagagcgtca atacatacag ggaggattta tacatagtgt tataattcag
     1801 gtcgaaaata cgtctaagta attcctcttg caatagggaa ttttacacaa caggtgcata
     1861 gtatttgacg ttatccatag tgaagattta ctattggcaa tggtaaacca ttaatctaat
     1921 tttaaaacct ggagtcagac tatgtagaaa cagaaaccgt atcaatgaaa acaaccatac
     1981 caaactgtat ccatccttac ctgcttgtta tcctggtggc tttgctgccg ggctccgttc
     2041 aggtcatggc ccagaatatt gactttggta aaagttatat taatgttacc aagggactga
     2101 acggtggcac tgttgaaaca ggggatacat tggaaataag ggcaacctct gtggtacgct
     2161 ccggaactta tgacagttgc cgttaccagg atgccgttcc tgcaagcacc gtttatattc
     2221 ccggcacagt cagggtgctg accaatgaag gaaaaatcta taaacagttt acagacacat
     2281 atggagatga tgagggctgg atcactggat caaatgtgac cataaacctg ggttacagaa
     2341 ccggtgctgc tccggccagt gcctttcgcc ggggacgggt ggccaacacc cataagcctt
     2401 ctttttatgg cagttcctgt atcatgatcg cttcattcag ggtccgggta acggcagcca
     2461 ccggatccaa tatcagtacc ggaggaggca atatgaccta taaactggga tctgcatcta
     2521 ttaacacatt cacttttccg gccaatacat tgcgtgtata tccaaactat ggcatttgtt
     2581 ccaattctat cggcaccaat gcaattggaa cagaattcaa cgggactttt ggaagtggcc
     2641 agcccaggaa ccgcggcaca tccggtaatg tgcctgttgg ttatacgtat aacttttttg
     2701 acctgaattc tccgcaggat tattattacg ggattgcgaa caacacttcc acccgttcaa
     2761 actataccac ttccaatagc tgggccaaac cagataattc gagtcccaca caccgggtgt
     2821 ttacagtatg ggatattatt ggtgatcata ccggtgcatt gaatccggta ctgggtaatc
     2881 cggcggctga tacggtggcc aatccgaatg cgggatatat gctggtgatc aatgcggctt
     2941 accggatcga ctccgctttc cagcaaacga tttcaggtct ttgcccgaac acgtattacg
     3001 agatctcctg ctggatgcgg aatatctgtt ccaaatgtgg ttgtgactcg aatggcaaag
     3061 gcgcttccaa ttcatccggg cctccctatt atatacccac cggaaccggt gattcatcgg
     3121 gtgtggcacc taatcttact tttgaaattg acgggatcga ttattataca tccggaaacc
     3181 tgatgtatag cgggcaatgg gtaaaaaaag gatttacttt cctgaccggt gcggcacaga
     3241 ccagttttac cctgaaattc tttaacaatg cccctggagg tgggggcaat gactgggcac
     3301 tggatgatat ttccgtttcc acctgttcgc ccaatatgag ttattcgcct tcgcttacgc
     3361 cgattgtttg tgacagtaac attctcacca tttatgacac ggtccgttcc ttctttaata
     3421 actacactta ctataaatgg caacggagta cggatggcgg gaccaattgg tctgatgtta
     3481 ccgctccggc gggacctgcc acgccggtat ggaacggtac cgcctgggaa tatgtaactt
     3541 cttataccgt acctgcatcc gaaacattac taacaaataa tggtgacctg taccgcctgg
     3601 ttgtagccac tacttccgcg aatctttcga atgcattgtg caattttacc gatgtagcca
     3661 atataatcgc gatgaatgtg attgactgcg ggatcccgct cgcggcacaa ttgttatcct
     3721 tcaatgggaa aaacaataac gcactggccg aattacaatg gacaacaagt gaggaagaag
     3781 aacccctgca gtttatcatc gaaaaaagtc cggacggcat tcacttttca gaaattggct
     3841 ctgtcaacag tcatctgaat aataccggag aagaacaaat ttataacttt acagatccgg
     3901 tagccctttc gggtaaaacc tattataggg tacgcataca gaatcaacgc ggacaatcga
     3961 tctggtccag aactattcag ctatcaaacc ggcaggagaa actcagtttt gtttccgtta
     4021 tcaacccctt ctcccatgaa ctgcagttgg atatcgctgc ttcacagtac agtaaatcag
     4081 aagccgaact gattgatgcc tccggtaaaa ttgtccggca taaaactgtg gatcttgcat
     4141 ccggcgtaac ccggatcaaa tttgaaaaca caatgggact ggcgcctggt atgtattttt
     4201 tgcgcatccg caaccaggat gggtatatac agaaaacagt attaaaaacg aatcagtagt
     4261 cgtcaggaaa cctgcatttt atcactgatc tcactgctgg tctccaccgt aggatttgca
     4321 tccgccggtt caggcttctt gaaaaaccgt ttttggaaaa tcagcaaggt gatcaccccg
     4381 atggagatag aagcatccgc tatattaaat atggggctga aaaaaacaaa gccattggtg
     4441 tcaatgattg ggaaataaag catatccacc acactcccat gcaggaagga agaataaccg
     4501 ttggatgaaa aggcagccac gcctgaataa ctgatgtagt cattcaatgc ggtatcataa
     4561 tgcaggcctt tatcaaagat catcccgtaa aacatactgt cgatcaggtt tcccagcgca
     4621 ccggcataga tcagtccggc acagataata aaccccttac tgtacttctg tttcacaatc
     4681 tgtccgaggt accaggttcc aaagatcacc gcagccaggc ggaaaagggt caggatcatt
     4741 ttgccggtct cattgccaaa tttccagccc caggccatgc ccggattttc aataaaatga
     4801 agccggaacc aatctgcccc gaatacccgc agcacttcac ccgtatcatg ggacgtttta
     4861 atccagattt tcagggcctg gtcagctatg ataataaggg caataaggag ggcaactgat
     4921 ctgagtttca atgacagaat tttaaggagc aaatataagg aaaaggagtc gggagagaga
     4981 agtcgggagt cgggagtcgg gagtaaacag cccctactga gaaaaggata gtaaaccctt
     5041 aagtccccct attcttcaaa gtagaccctt ttaacccgtt gggaaacgcc ggtgaggatt
     5101 tcatagggga tggtatctgc ccatcgggcc agttcggtga ctggtagctc tttaccgaat
     5161 ataatcactt catcaccctg agtgacccct ggaatggccg tcacatccgc catgaacata
     5221 tccatacaaa cagtacctat gaccggggcc agttttccat tgatcagcac actgcctttt
     5281 ccattaccca gccggcgggg atagccgtca gcataaccca gccggatcgt ggcgatcagc
     5341 gaggctttct ctactctccc tttccggtta tagctgacag aatcgccggg cttcagccat
     5401 ttcagctgtg caatggttga ctttaaggtc gccactgttt gcaagtccaa cgcagcggta
     5461 ccggcgctat ctacaccata taggccaatc ccca
//
LOCUS       DAOPMW010000221         5414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17521_length_5414_cov_8.410711, whole genome shotgun sequence.
ACCESSION   DAOPMW010000221 DAOPMW010000000
VERSION     DAOPMW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17521_length_5414_cov_8.410711"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLU11_10480"
     CDS             complement(<1..316)
                     /locus_tag="PLU11_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPN59594.1"
                     /translation="MFAEVIIPAALPKNYTWSVPAHLQETARPGCRVEVNLGKSKKYA
                     GVIKRLHEEKPEYFETKDILNVLDVEPVVFEEQLKLWEWIASYYMRSEGEVMAAALPS
                     HFK"
     gene            complement(613..1371)
                     /locus_tag="PLU11_10485"
     CDS             complement(613..1371)
                     /locus_tag="PLU11_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN59595.1"
                     /translation="MKGLILLLKAVYSIYAFIVFVAIMLLVFPFSILASFFGRIKGGN
                     LMMRLCMIWADTWFLLIGMPHPKIYESKHDKKKAYIFVSNHISYLDAAIIPKAIRQPV
                     RPLGKVEMSKLPVFGFIYRNAIVTVDRSSPANRANSVRILKSLINKGISVLVFPEGTF
                     NMTRHPLKEFYDGAFRVAIETQTPVKPVLFLDAYRRMPYESIFRMSPGRSRILYLEEI
                     PVEGLSMADVQVLKEQVYRIMEEKLVEYNAVGSR"
     gene            complement(1372..1845)
                     /locus_tag="PLU11_10490"
     CDS             complement(1372..1845)
                     /locus_tag="PLU11_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPN59596.1"
                     /translation="MNKKWVPYLLMGLMLLVIIVVKQCRNSPSPAPKPVVTDNNRPKD
                     PASNPTDRDKGFDRRVSYLEYSNHAKCRMQCRKISQAEVEEIMRDGKINYNKSDLQNA
                     RCPRYAVEGLTRDDQRVRIVYAQCNDKTVVVTVIDLETDFKCDCPGDDDKYKNKK"
     gene            2008..3300
                     /gene="mtaB"
                     /locus_tag="PLU11_10495"
     CDS             2008..3300
                     /gene="mtaB"
                     /locus_tag="PLU11_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPN59597.1"
                     /translation="MSESRTVAFHTLGCKLNFSETSSLSRMLELEGFEKKEFDDLADV
                     YVINTCSVTENADKECRQLVRRIQRKAPESFVVITGCYAQLKPQDIVSIPGVDLVLGA
                     AEKFNIARHIRELAKGDPAKICSCDIEEVSGFHSSWSVNDRTRTFLKVQDGCDYTCSF
                     CTIPMARGKSRSDRIANVVRNAEELAAGGVKEIVLTGVNLGDFGKGPDGSGATMPLSG
                     REENFFELVKELDKVAGISRYRISSIEPNLLTNEIIEFVANSDKFMPHFHIPLQSGSN
                     KTLGAMRRRYKRELYTERVSLIKKLMPHCAIGVDVIVGFPGETEDDFRETYEFLHSLD
                     VSYLHVFTYSERDNTQALEISPVVPMSVRNERNKTLRNLSYMKMQYFTGQHQGQSRKV
                     LFEHQDKNGMMEGYTDNYIRITTPWREEWVNNIVAWNI"
     gene            3347..3421
                     /locus_tag="PLU11_10500"
     tRNA            3347..3421
                     /locus_tag="PLU11_10500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3382..3384,aa:Val,seq:gac)
     gene            3727..4017
                     /locus_tag="PLU11_10505"
     CDS             3727..4017
                     /locus_tag="PLU11_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59598.1"
                     /translation="MQKYQVTIQFEVSEAFMEQVPAHRTYINFLINKGTIDHYAVSME
                     SHRSWITLNAESKKEVEKILKKSPLSVFWTFEIDELFVLDGQHYRLPEVNPN"
     gene            complement(4014..4580)
                     /locus_tag="PLU11_10510"
     CDS             complement(4014..4580)
                     /locus_tag="PLU11_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPN59599.1"
                     /translation="MAATYCEYVDNKGKNTVHQHYHDKEYGFPLINDDQLFGRLVLEI
                     NQAGLSWETILKKKEHFFKVFDGFRIEKVARYSEAKKAKLLNDPGIIRNRLKVEAAIY
                     NAGQILIIQKEYGSFLNWLDHHHPLTREEWVKLFKTRFRFTGGEIVNEFLMSTGYLPG
                     AHVETCLIYKKVLKCKPRWATKKGNGKG"
     gene            4765..>5414
                     /locus_tag="PLU11_10515"
     CDS             4765..>5414
                     /locus_tag="PLU11_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPN59600.1"
                     /translation="MKILYKTQWSLLFRPGISRTKLFLLILFIAAGNVVSAQNALDFP
                     TGAASNTNYVSLPNLIPSGSYTKEAWINGRSFASGTNNILSGSNSAFWVVNGQLCAGH
                     NAVYTGIFDVTGFSTSQWYHVAVTYDAGTNFMQLYIDGAAVAGAGSGNPVGVYSETNA
                     YIGAFNDGSQIAYQFDGQIDEVRVWNVVRTATEIANNRSCGLTGDEPGLLAYYRFN"
BASE COUNT         1459 a         1252 c         1165 g         1538 t
ORIGIN      
        1 atttaaaatg cgaaggcagg gcggccgcca ttacttcccc ttcactacgc atataataag
       61 aggcgatcca ttcccagagt ttcagctgtt cttcaaatac caccggctcc acatccagca
      121 cattcaggat atccttggtt tcgaaatatt ccggcttttc ctcatgcagc cgcttgatca
      181 ctcctgcgta ctttttactc ttacccaggt tcacttccac acggcaaccc ggccgggccg
      241 tttcctgcaa atgcgcaggc accgaccagg tgtagttttt gggtaatgca gcaggtataa
      301 ttacttcagc aaacaagaat atatttttta aaagaaccaa gaaccaaaga tgccaagaag
      361 ccaaataaga accaagaaat aaaatccaac taaaaatcaa gtcacaaata acaaatcctc
      421 ctaagaagtt tttggtgcat atttttattt catagtgagc cggtactttt gtcatgctga
      481 gcctgcctgt ccggtaggca ggcctgccga agcacgacca accaatatgc actttttctt
      541 actcaccctt cgacacgctc agggtgacaa gagcattccg cttagtaccg gctgttgact
      601 cctgactgca gactaccgac tgccgactgc attatattca accagcttct cctccataat
      661 ccggtaaacc tgctctttta acacctgtac atcggccatg gacagacctt ctaccggtat
      721 ttcctctaag tacaagatcc ggctgcggcc cgggctcatc ctgaaaatac tttcatacgg
      781 catccttcga taggcatcca ggaagagaac cggttttacc ggggtctggg tttcgatggc
      841 gacccggaat gctccgtcat aaaattcctt gagcggatgt ctggtcatat taaatgtacc
      901 ctctggaaaa acgagaaccg aaattccttt attgataagc gacttcagta ttcgtacact
      961 attcgcccgg ttagccggac tactgcggtc aacggtaaca attgcattcc ggtaaataaa
     1021 tccaaagacc ggcagtttac tcatttccac ttttcccaat gggcgtaccg gctgacggat
     1081 ggctttgggt attatagccg catcgagata ggaaatatga ttgctgacaa aaatgtaagc
     1141 tttcttttta tcatgtttag attcatatat tttcggatgc ggcattccaa taagaagaaa
     1201 ccaggtatcc gcccatatca tacataaccg catcatcagg ttgcctcctt tgatccggcc
     1261 aaaaaaactg gccaggatac tgaaggggaa tacgagcagc atgatggcca cgaatacgat
     1321 aaacgcgtaa atagaataaa ccgccttcag caacaatatc aagcccttca tctatttctt
     1381 gtttttatat ttatcatcat cgcctggaca atcacattta aaatccgttt caaggtctat
     1441 tacggttact accacagtct tatcattgca ttgtgcatac acgattctta cccgttggtc
     1501 atcccgggtg agcccttcca cggcataacg tggacaacgg gcattttgca gatcgctttt
     1561 attataatta atcttcccgt cccgcatgat ttcttccact tctgcctgtg aaatcttccg
     1621 gcattgcatc cggcacttcg catggttgga atattccagg taggataccc gtcggtcaaa
     1681 gcccttgtcc cggtctgtcg gattgctggc cggatctttg ggacggttat tatcggtcac
     1741 cacgggtttg ggagccggtg acgggctgtt cctgcattgt ttaacgacaa taatcaccag
     1801 cagcatcagc cccattaata aatagggaac ccacttctta ttcactttca aacatttgga
     1861 taaaaataag gaaattgggc aggatggggt cagtttggtt gctcttcatt tccccggctc
     1921 ccgtcttcct cgtatctttg ccggctccct gcattaaccc cgtccctaaa cttatggacg
     1981 taatgaaaaa tggcagggaa atcaggtatg tcagaatcaa gaaccgttgc ctttcataca
     2041 ctcggttgta aacttaattt ttcggaaact tcttcccttt cccggatgct ggaactggag
     2101 ggttttgaaa agaaagagtt tgacgacctg gccgacgtgt atgtaatcaa tacctgttcg
     2161 gttaccgaaa atgcggataa ggaatgtcgt cagttggttc gaagaattca acggaaagcg
     2221 cctgaaagtt ttgtagtgat cactggctgt tatgcccagc tgaaaccaca ggatatcgtt
     2281 tcgattccgg gggtggacct cgtactgggg gctgccgaaa agtttaatat cgcccggcat
     2341 atccgggaac tggccaaggg agatccggca aagatctgca gttgtgatat tgaagaagtc
     2401 tccggttttc attcttcctg gtccgtaaat gaccgtaccc gcacttttct taaagtgcag
     2461 gatggctgtg attatacctg ttctttctgc accattccta tggcccgggg caaaagccgg
     2521 agtgaccgta ttgccaatgt agtccgtaat gcggaggaat tggccgcagg cggcgtgaag
     2581 gaaattgtac taacgggggt caatctgggg gattttggga aaggaccgga tggcagcggg
     2641 gctaccatgc cgctaagcgg ccgggaagag aatttctttg aactggtcaa agaactggat
     2701 aaagtggccg gcatcagtcg ttaccggatt tcctcaatcg agccgaacct gttaaccaat
     2761 gaaatcattg aatttgtagc caatagcgat aagtttatgc cccattttca tattcccctg
     2821 caaagcggca gcaataagac cctgggggct atgcgcagaa ggtacaaacg ggaattatat
     2881 acggagcggg tcagtctgat taaaaaactg atgcctcatt gtgcaatcgg cgtggacgtg
     2941 attgttggat ttccaggaga aacagaagat gatttcaggg aaacctatga atttcttcat
     3001 tcactggatg tctcctacct ccatgttttc acttattcag aacgagataa tactcaggca
     3061 ctggagatca gtccggtggt acccatgtct gtccggaatg aacggaacaa gacgctgcgt
     3121 aatctttctt acatgaagat gcagtatttt accgggcagc atcagggaca gtcccgtaaa
     3181 gttctttttg aacaccagga taaaaacggg atgatggaag gatatacgga taattatatc
     3241 cgcatcacca ctccctggcg ggaagaatgg gtcaataata ttgtggcatg gaatatttaa
     3301 gtaaagcgct tatttttaca gccccgtttt aaatgggtta ttaaccggaa cgttagctca
     3361 gttggttcag agcattactt tgacagagta ggggtcactg gttcgagtcc agtacgttcc
     3421 acgttcttct ccgaagcatt tccaccttcg ttaaaacttc ggtggatgaa aagcgaagga
     3481 gaactaagcc ctaaaagata atcatctttt agggtttttt ttaccccaga ttattcagca
     3541 agtcaaaatt caaaagtaaa aagtaaaaac ctctatagta gtaaattccc tgccggcttc
     3601 attactgatc ttggccgccg tctgcctact gccgactgcc tactgccgac tgttacctgt
     3661 gtctgcataa ctttctttcc cctcccgctg caactttcct acatttaagt tgtctttata
     3721 tatagtatgc agaaatacca ggttaccata cagtttgaag tgagtgaggc atttatggaa
     3781 caggttccgg cccaccgtac gtatatcaat ttcctgatta ataaagggac gattgatcat
     3841 tatgcggtga gtatggaatc gcatcgttcc tggattaccc tgaatgcaga aagtaagaaa
     3901 gaagttgaaa aaatattgaa gaaatccccc ctcagcgtct tctggacatt tgaaattgat
     3961 gagttgtttg tcctggacgg gcaacattac cggttgccgg aagtcaatcc gaattaaccc
     4021 tttccattcc cttttttagt ggcccatcgg ggtttacatt ttaatacttt tttatagatc
     4081 aggcaggttt ctacatgggc acccgggaga taacccgtgc tcatcagaaa ttcattgacg
     4141 atttctccac cggtaaaacg gaaacgggtc ttaaaaagtt tcacccactc ctcccgggta
     4201 agtggatgat gatggtccag ccagttgaga aaggatccat actctttttg aattatcagg
     4261 atttggccgg cattataaat agccgcctct actttcagcc ggttgcggat aatccccggg
     4321 tcgttcagca attttgcttt ttttgcttcg ctataccgcg ctactttttc aatacgaaag
     4381 ccatcaaaga ctttaaaaaa atgttctttc tttttcaata tagtctccca gctcaaccca
     4441 gcctgattga tttccaaaac gagccggcca aataattgat cgtcattaat aagtggaaac
     4501 ccgtattctt tatcatggta atgctggtgg acggtattct tacctttgtt atccacatat
     4561 tcacaatatg ttgcagccat ttcaagagat tttatacaat tatcagcaaa aaaactgatc
     4621 tgcatttcaa actcggtgtt tctacttaaa ttaaatttta tagtatagta taattttata
     4681 cttttagagc agaaatccat cctgacaaaa tccacttaat cggtacagtt tcctacgcta
     4741 ttaaacaact aaaaattgaa ttgcatgaaa attctttaca aaactcagtg gtccttattg
     4801 ttcagacccg gtattagccg gacaaagcta ttcctgttaa ttttatttat cgctgccggg
     4861 aacgtcgttt ccgctcagaa tgccctggac tttcctacag gcgcggcttc taataccaat
     4921 tatgtgagcc tgcccaactt gattccaagt ggttcttaca ccaaagaagc ctggatcaat
     4981 ggccgttcat ttgccagcgg aaccaataat attctttccg ggtcaaattc tgctttctgg
     5041 gtagtcaatg gccaattatg tgccggacat aatgcagttt atactggcat attcgatgtt
     5101 acgggtttta gtaccagcca atggtaccat gtagcggtaa cctatgatgc cggcaccaac
     5161 tttatgcaac tttatattga tggagcagct gttgccggag ccggatctgg taacccggtt
     5221 ggcgtataca gtgaaaccaa cgcttacatt ggtgcgttta atgatggatc acaaattgcc
     5281 tatcaattcg atggccagat cgatgaagta agggtttgga atgtagtacg taccgccact
     5341 gaaattgcca ataaccgcag ttgtggatta accggtgatg aacccggctt attagcttat
     5401 tatcgtttta acca
//
LOCUS       DAOPMW010000222         5401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17581_length_5401_cov_6.901983, whole genome shotgun sequence.
ACCESSION   DAOPMW010000222 DAOPMW010000000
VERSION     DAOPMW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17581_length_5401_cov_6.901983"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLU11_10520"
     CDS             complement(<1..661)
                     /locus_tag="PLU11_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59601.1"
                     /translation="MNRNKTGVALMGCLLLFSAFIAADTIITRLGLEHKTARYYILSN
                     LAGSFEEGPMEENEEDGGSNADAYDQMKSFRIPYARLLPAVIKGDKNGAARELCSYVK
                     MYVNSSDFMNDYNKRRDAAKPTTEPWRPDAEMIQSQRQSVKEMDKQLADLKKQKGISP
                     DIIAAYEKGIQDQKKNLANWEDPHPNLTRWEKHYPADPAPLVKQKLQDYLALVASVDF
                     DA"
     gene            complement(683..1972)
                     /locus_tag="PLU11_10525"
     CDS             complement(683..1972)
                     /locus_tag="PLU11_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59602.1"
                     /translation="MKKFFPLLFLFLLIESAMAQNESSPAGWITIDLNSFMCQKATND
                     DFLNFDGFGDEVYFVIYYSVLDKNGVTRYTNKVVSKTYGDTYRFPDRVLAGHANPDNK
                     GGLSTGSQFFPGNEFPNIRRVRLEAGDYITVIPTIWEWDNNSNSQLQNSIDSRIINSF
                     NSINQPIPGILQYCYGSYKCYQFNNLSVIRMPTFADLLKSVNGVNGSRPIGISKAGEF
                     SPIVFSLKSQMIITQNKNNPIDGKTYSMSYNDFVINEESMGNTRDHGIYMLRFHFTFE
                     KDLSKPAPPPAPAPAGNNNVFVNPPRPVKQSIPVKTTGIKIASVPVNNELMSGVWKGT
                     TGTMTSATDGPLNFKCSNYGFWLLNNNGSGTAVVAGGYNFENGNFTGSYTDSNNWKWI
                     YTSTGYNTQTGELSGSWTCTGSGYYKTGKWIMKKISN"
     gene            complement(2122..3435)
                     /locus_tag="PLU11_10530"
     CDS             complement(2122..3435)
                     /locus_tag="PLU11_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HPN59603.1"
                     /translation="MFKKITFTGLILFCLAMSVLAQRVQPQKVGKPVQLERPVKLNQA
                     ILVQDPVRMCVDRYPSTGNFPPRDNSQVQPPPRINADGTVPSVGVTRQPLAGETMKMW
                     NPGQVIGVYLSTNNGSELIRQKVITYAKKWETIANIKFSFTNNINDAQIKVEFSDDNR
                     SWSWIGRDVLFNPLGFYTMHFGWFDSNTKETEFKKTILHEFGHALGFIHEHQSPANNI
                     QWNKPEVYKYYAGPPNNWSGIEVDINLFAKYNAGSTNSSRYDPKSIMHYSIDKGLTLD
                     GFTVGVNYDFSPMDITFARQVYPFPFKPGNSSGLLQTGDDCDMVDFTVEYDVVASDKI
                     EFILELGKRNNVAVTWWKQISIPLRNNRKQELWVQNHSLIASENLTWTNALINVNDLD
                     KANGIAFWKAKAFGVHTLLGYRWNVLSALPGGSRVRLVWKNDSCR"
     gene            3688..5391
                     /locus_tag="PLU11_10535"
     CDS             3688..5391
                     /locus_tag="PLU11_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59604.1"
                     /translation="MNWRKIILTSVTALFCVPLLLWGQGTPKTDSLLKALAVAKKDTS
                     KILLLYAIAEQYENSEPDKARKYIKQGGELSREINYQPGIMKSYRMLSYVYSFQSKFD
                     SVIYYNRLVLDIARSRNDSFNIGVSFFNIGIGFRFMSELDSAVVYTLQGAKLLEGKGY
                     SNIESSINDGLQTLYMTLGQYEKAISYGNKAVELARKLEDQSALANSLSNLGLSYSET
                     NRVSEAKKIYLEALQIAESTNNKSIQAVVLNNLSDIAIKENQFDRLKTYAERSIALAG
                     EMEDEGTLISGKLSLAAYLLSRQEYVAAQEQALAALALSEKQNLLEGKTTSLGMLSAI
                     AFSRQDYKKGFEYYYQKMDFEGKVFNQSLQQKEAALRIRYETEKKDTQILLQRTELQR
                     KTVFNYLLIGGAAALLLITLLSYRTYRQKQKLQQHRIEELETQQQLLAAEAVMKGEEQ
                     ERTRLAKDLHDGLGGMLSGIKYSFQTMKGNLIMTPENQQAFERSMDMLDSSIKEMRRV
                     AHNMMPEVLVKFGLDTAMKDFCDDINRSGALRVVYQSLGMDLLQVEQTAAIAVYRIVQ
                     E"
BASE COUNT         1411 a         1151 c         1275 g         1564 t
ORIGIN      
        1 ctgcatcaaa atccacggaa gcaacaagcg ccaggtagtc ctgaagtttt tgctttacca
       61 atggggcagg gtctgccgga taatgtttct cccagcgggt aaggttcggg tggggatctt
      121 cccagttggc caggtttttt ttctggtcct gaattccttt ttcataggca gcaataatat
      181 ccggcgatat tcctttttgt ttttttaaat ccgccagttg tttatccatt tcttttacag
      241 attgacgctg gctttggatc atttcagcat ccggtcgcca gggttcggta gtgggtttgg
      301 cggcatccct tcttttgttg taatcgttca taaaatctga actgtttaca tacattttta
      361 cataggaaca aagttcccgg gccgccccgt tcttgtctcc ttttatgacg gctggcaaca
      421 gacgggcata gggaatgcgg aatgatttca tctgatcata cgcatctgca ttactccctc
      481 catcttcctc attttcttcc atcgggccct cttcaaaact tccggccagg ttgctgagga
      541 tataatagcg ggctgtttta tgttcaagtc caagccgggt tatgatcgta tctgcagcga
      601 taaaagcgct gaaaagcagc agacagccca ttaaggcaac cccagtttta tttctattca
      661 tggtaaaatg gtttattttt tcttagttgc ttattttttt cataatccat tttccggtct
      721 tataatatcc ggaaccggta caggtccagc tgccgctgag ttcacctgtt tgtgtgttgt
      781 accccgtgga agtatatatc catttccagt tattactgtc agtatagctt cctgtgaaat
      841 ttccgttttc aaaattatat ccccctgcca ctaccgctgt tccactgccg ttgttattca
      901 gcagccagaa tccatagttg ctgcatttga agttcaacgg accgtctgtc gcactggtca
      961 ttgttccggt tgttcctttc cagacacccg acatcagttc attattaacc gggacagatg
     1021 ctattttaat acccgtggtt ttaaccggaa tggattgttt aaccgggcgg ggtggattaa
     1081 cgaatacatt gttgtttccg gcgggagcag gtgcgggtgg tggtgccggt ttgctgagat
     1141 ccttttcaaa cgtgaaatga aaccgcaaca tatagatccc atgatccctg gtattcccca
     1201 ttgattcttc atttatgaca aaatcgttat aactcatgct ataggttttg ccatcaatcg
     1261 ggttgttttt attctgcgta ataatcattt gcgatttaag cgaaaaaacg attgggctaa
     1321 actcaccggc tttacttatc cctatcggac ggctgccgtt gaccccatta accgatttaa
     1381 gaaggtcggc aaaagtgggc attcggatca cacttaagtt attgaactga taacatttat
     1441 aactcccata acagtattgc agtattcctg gaatcggctg attgatacta ttgaatgaat
     1501 tgattatacg gctgtcaata gaattctgta gctgactatt gctgttatta tcccattccc
     1561 agatcgtcgg gattaccgtt atataatccc cggcttccaa ccggacacgg cggatattgg
     1621 gaaattcatt accggggaaa aactggctgc cggtggagag ccccccttta ttatctgggt
     1681 tggcatggcc agccagcacc cggtccggaa aacggtacgt atccccgtaa gttttggaca
     1741 ccactttatt tgtatagcgg gtaaccccgt ttttatccag gaccgaataa tagatcacaa
     1801 aatagacttc gtcgccaaag ccatcaaaat tcaggaaatc atcattggtg gctttctggc
     1861 acataaagct attcaggtca atagtgatcc agccggctgg tgaggattca ttctgggcca
     1921 tggcggattc aatcagcaga aataagaaca acaacgggaa gaactttttc ataattgcaa
     1981 tttgttgtgt aaatatggaa tttaccggtg agccaggtta tccctgaaaa ccatgaattt
     2041 tagcgaaaac ggtacatgcg aaagtcctgc aaacagcgtg tttcagcgat tggcaggact
     2101 ttcagtagat attgccgggt tttaccggca ggagtcattt ttccatacca gtcttaccct
     2161 tgagccacca ggcagtgcgc tcagcacatt ccagcgatag ccaagcaggg tatgtacccc
     2221 aaaggctttt gctttccaga atgcaattcc attcgctttg tccaggtcat ttacattgat
     2281 aagtgcattg gtccaggtca ggttttcaga agcaatcaat gaatggtttt gcacccatag
     2341 ttcctgtttc cggttgtttc ttagtggtat gcttatctgt ttccaccagg taacggccac
     2401 attgttcctt ttgcccagtt caaggatgaa ttcaattttg tcactggcca caacatcgta
     2461 ttcgacggtg aaatccacca tgtcgcaatc atcaccggtt tgcaacaggc cgctgctgtt
     2521 acctggcttg aaggggaaag ggtatacctg gcgggcaaaa gtgatgtcca tcggagagaa
     2581 gtcataattc actccaactg taaatccatc gagggtcagt cctttatcga tcgaatagtg
     2641 catgatcgat ttgggatcat acctggaaga attggtactc cccgcattgt attttgcaaa
     2701 gagattgatg tccacttcta tacctgacca gttattaggc gggcctgcgt agtacttgta
     2761 tacctctggt ttattccact gtatattatt ggctggtgac tggtgttcat gtataaatcc
     2821 gagggcatgg ccgaattcat gcaaaatcgt ttttttgaat tctgtttctt tcgtattgct
     2881 gtcgaaccat ccaaagtgca tggtataaaa accgagcggg ttgaacaaaa catcacgtcc
     2941 tatccaggac cagctcctgt tgtcgtcaga gaactccact ttgatctgtg catcattaat
     3001 gttgttggtg aagctgaatt taatattggc gatcgtttcc catttttttg cataggttat
     3061 cactttttgc cggatcagct cggagccgtt attcgtactt aagtaaacgc cgatgacttg
     3121 tcccgggttc cacatcttca tggtttcacc tgcgaggggt tgtctggtta cgccaacgga
     3181 aggtaccgtt ccatccgcat tgattctggg tgggggctgt acctgtgagt tgtctcttgg
     3241 agggaaatta cccgtggaag gatatcggtc cacacacatt ctgaccgggt cctgaaccaa
     3301 tatggcctga ttcagtttta ccggacgttc aagttgtacc ggtttgccaa ccttttgagg
     3361 ttgtacccgt tgggcaagaa cggacatcgc caggcaaaac aggatgagtc cggtaaaagt
     3421 tatttttttg aacatagttt agtgtttatt tggttaatgg taatacaagg acctcacccc
     3481 gggtttaaag ggcagcttga tttttttcat atcatacatt ataaagattc aagcatcttt
     3541 tctgacccga agaggtaaaa ctgtttttat tttatgatag ttcctatcct gtaaaaacag
     3601 gaatttccac aggtatcgaa aaatccctta aaaaggggag gctgcatacg gggttgtatc
     3661 ctttatttgc agtataatgc tggaaaaatg aattggagga agattatatt gacaagtgtt
     3721 accgcactgt tctgtgttcc tctgctgctg tgggggcagg gtaccccaaa gacggatagt
     3781 ctgcttaaag cgttggcagt tgccaaaaaa gataccagta aaattttatt gttgtatgct
     3841 atcgcggagc aatatgaaaa ttctgaaccc gataaagcga gaaaatatat taaacagggc
     3901 ggtgaattaa gccgggagat caattatcaa cccggcatta tgaagtcata ccggatgctt
     3961 tcttatgtat actctttcca gtcaaaattc gattcagtta tttattacaa tagactggtg
     4021 ttggatatcg cccgcagccg gaatgattca tttaatatcg gggtttcatt ttttaatatc
     4081 gggatcggct tccggtttat gtccgaactg gatagtgcgg tggtttatac cctgcagggg
     4141 gcaaaattac tggaggggaa ggggtacagt aatattgaaa gcagcataaa tgacgggctg
     4201 cagaccctct atatgaccct gggtcaatac gaaaaagcga tcagttacgg gaacaaagcg
     4261 gttgagctgg cccgtaaact ggaagaccag tctgcactgg ctaacagtct ttctaacctg
     4321 gggctttctt attcggaaac caatcgggta tcagaggcaa agaaaatata tctggaggcc
     4381 cttcagattg cagaatcaac taataataaa tccatccagg cggtggtgct gaataatctt
     4441 tcggatatcg ctattaagga aaatcagttt gaccggttaa agacatatgc cgaaagaagt
     4501 atagccctgg caggcgaaat ggaagatgag ggtacactga tcagtggcaa actcagcctg
     4561 gcggcttatc tgctgagccg gcaggaatat gtggcggcac aggaacaggc tttagcagct
     4621 cttgctttgt ctgaaaagca gaacctgctg gaggggaaga ccacttcgct gggtatgctt
     4681 tctgccattg ctttttcccg gcaggattac aaaaaagggt ttgaatatta ttaccagaaa
     4741 atggattttg agggaaaggt attcaatcag tcgctccagc aaaaagaggc ggcactcagg
     4801 atccggtatg aaacggagaa aaaagataca cagatactgc ttcaacggac cgaacttcaa
     4861 cggaaaacag tttttaatta tctcctgatc ggcggcgcgg cggccctgtt gctgatcaca
     4921 ctcctttctt atcgtacgta caggcagaag cagaaattac agcaacaccg gatcgaagaa
     4981 ctggaaacac agcaacaatt attggcagcc gaagcagtaa tgaaagggga agaacaggag
     5041 cgaacccgcc tggcaaaaga cctccatgac ggattaggag ggatgttgtc gggtattaaa
     5101 tactcatttc agactatgaa aggaaacctg attatgacgc cggagaacca acaggcgttt
     5161 gaacgaagca tggatatgct ggacagttca attaaggaaa tgcgccgtgt ggcacataat
     5221 atgatgcctg aagttttggt gaaattcgga ctggatacgg ccatgaagga tttctgtgat
     5281 gacattaaca ggtccggagc cctgcgggtg gtttatcagt cattggggat ggacctgtta
     5341 caggttgaac agacagccgc tattgctgtt taccggatag tacaggaata gcggctgtct
     5401 g
//
LOCUS       DAOPMW010000223         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17589_length_5399_cov_7.057260, whole genome shotgun sequence.
ACCESSION   DAOPMW010000223 DAOPMW010000000
VERSION     DAOPMW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17589_length_5399_cov_7.057260"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLU11_10540"
     CDS             <1..1122
                     /locus_tag="PLU11_10540"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPN59605.1"
                     /translation="AYAAEFQKRVNANPAFKEQYATLLGDLEAAYQELNKYGLARDYY
                     TETISRIEAFALGSRINSLAGIKQKNGDKAFKDAVPKTIETLGELYKEYSPAVDKKLF
                     EALMQKFTNDQPEEFLAPELLTLVKSYQSDFAKMADDFYTNVDLLNNGPKVLANLKAN
                     PDLVLMANDLNKANQLFLQLNSHYASTVTKKLSGIQARVNQLQRTYMKAQMEVMKEKK
                     FYPDANSTLRVTYGNVKGYQPRDAVKFDFYTYLDGVMDKYKPGDYEFDVPEKLRTLYK
                     NKDFGQYGKNGKMPVCFIAMNHTTGGNSGSPALDANGNLVGLNFDRAWEGTMSDINYD
                     PSICRNIMVDIRYVLFIVDKYAGAGHLVQEMKLVRAGKK"
     gene            1319..3469
                     /locus_tag="PLU11_10545"
     CDS             1319..3469
                     /locus_tag="PLU11_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPN59606.1"
                     /translation="MSIIQDIRDKYAKLTVVLIALALVGFILTDYFAGKSRGMVGGPS
                     NTVGVVNGKKILFEDFSKKVTQAEENMKAQGYPPAAASQQALDQAWSQEINQAILSDE
                     FEKLGIAVGRKELGDILYGQNAPQDIKQQFTDEKTGQFNAALAKQNIDQKLKSGTAEE
                     KANINAYINQLVNLRMADKFFSLMSGTINFPKWMIEKQNAENAQMARISLVRETYASI
                     PDSSIKVEDSEIEAFVNKNKKLFKQAETRSISFVSFSAAPSAKDSAATLSSLVTLRDR
                     FASSDSVAQFLAGEGAQYFDAYVSGNAIQIPVKDSIFKLPVGGVYGPYLDANSYVLAK
                     MIGVRVLPDSVKCRHVLVSNNLQQGGVDDSIASRRIDSIKLAINGGANWDSMVQKYNP
                     PSDASRQNKGEMTFSSTEIQGPNFAREFGQFILLDGKVGERKVVKTSFGYHFIEIMSA
                     IKPSTHYKVAYLPKEIIASQETDNAALNEANSFAAESTDLKSFDDTYEKKLKAKGFQK
                     GIATDILRASTSIRGLENASRSFVRDIYAAKRGEVLKPERVDNSYVVAVVTEALEEGT
                     MSTARARAAGAETEVRVKKKGELLKKKIGTVTTLEDAIAKLGGLKQIEPIDSLRIYGS
                     NLSYEPKVIGAAFNPANKGKVVPEVLVGKQGVYVIRVEDQSSTAVPAEDINAVRLSRY
                     QQAKQSLGNQYSQNNPLTILRNAADIKDKRSERF"
     gene            3587..4111
                     /locus_tag="PLU11_10550"
     CDS             3587..4111
                     /locus_tag="PLU11_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HPN59607.1"
                     /translation="MSEPFVNKVADSGLITLDLEKWYPREETAVFDIKDYLFMGMILK
                     EKDFREALKAKDWTIYQDKNVALTCSVDAIIPVWAYMLVAANLQPFANEIVMGDEKEL
                     HKTIFLKNLSAIDVQEFYDKRVVIKGCGETPIADYVYMEITKLLRPVAKSIMYGEPCS
                     TVPVFKKQGGPKKD"
     gene            complement(4108..>5399)
                     /locus_tag="PLU11_10555"
     CDS             complement(4108..>5399)
                     /locus_tag="PLU11_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPN59608.1"
                     /translation="ISSLTKGEAVINVMNYLSLDAACIGNHEFDHGWENVPVQLKKAR
                     FPVVNGNIFLKGKKKLIWNNPYTIIQMDGIRIGVIGLHGKFAFYDTIADEMIQGVEAR
                     NEEIYLKKYIKELRKKADLIILLVHQGIPGRQSSTGSTDVARNLQRDIDLAAAIPGID
                     IMITGHAHQGTPQALLSNGTIIVSTDALGSELGKLEINYHPVKDKIIAHKNTLGYLFD
                     DEVADDSATAKAILVWKEKVKKITEEEVTTIPFALTRSYGEESLMGNMVADAILYAYP
                     GFDLAVTNSGGLRQDIEAGTVTTGNIISAFPFPNTIHQLELTGADLYELFEHGAALTN
                     GILQVSKGVEIVYNETLPVGSRITSCKLNTEELNRNKIYKVLTSNFLADGGDGFLAFK
                     KAVSRKKTTLEIVQAMISYLKTFKEYKPVLEGRIKKS"
BASE COUNT         1578 a         1127 c         1240 g         1454 t
ORIGIN      
        1 gcctatgcgg ccgaatttca gaaaagggtc aacgccaacc ctgcctttaa agaacagtat
       61 gctaccctgc tcggtgatct ggaagccgcc taccaggaac tgaataaata tggcttggcc
      121 agggattatt acactgaaac tataagccgt attgaggctt ttgcacttgg ttccagaatc
      181 aatagtcttg caggcatcaa acaaaaaaac ggtgataaag cgtttaaaga tgctgtgcct
      241 aaaacgattg aaacactcgg tgaactctac aaggaatata gtccggcggt ggataagaaa
      301 ctctttgaag cattgatgca gaaatttacc aacgaccagc ctgaagagtt cctggcaccg
      361 gaactattaa cactggtaaa gtcctatcaa tccgattttg caaaaatggc ggatgatttt
      421 tacacgaatg tagacctgct gaataacggg cctaaagtac tggccaacct gaaggcaaat
      481 ccggatctgg tgctgatggc caatgacctg aataaggcta accagttgtt tttacagtta
      541 aacagccatt atgcatcgac tgtaacaaag aagttgagcg gtatacaggc ccgggtgaat
      601 cagttgcagc gtacctatat gaaagcccag atggaggtga tgaaggagaa gaaattctat
      661 cccgatgcca acagtaccct ccgtgtcact tatggtaatg taaaaggata tcagcccaga
      721 gatgccgtga aatttgattt ctacacttat ctcgatggag tgatggataa atacaaaccc
      781 ggcgactacg aatttgatgt tcctgaaaag ctcaggacct tgtataaaaa caaggatttt
      841 ggccaatatg gtaaaaacgg gaaaatgccg gtttgtttta tcgccatgaa ccatactaca
      901 gggggaaatt ccggaagtcc cgctctggat gctaacggca acctggttgg actcaatttt
      961 gaccgtgcct gggagggaac gatgagcgat atcaactacg atcccagtat ttgccggaat
     1021 attatggtag atattcgtta tgtgttattt atcgttgaca aatacgccgg agcgggtcat
     1081 ttggtccagg aaatgaaact ggtcagggct ggaaaaaaat aggattctgg cgaatagctt
     1141 tccaaaaaaa acgggctgac ccactaaata cgggtcagcc cgtttaattt ccctgaaaac
     1201 agcccccatg cccgtttttt catctacttt caccctcaaa acgggtgcat taattataga
     1261 ttttccacag tttttcccct atttttgcca tccttaaaaa aatcagtaat aatatattat
     1321 gtctattatt caggatatcc gagacaagta cgccaagctt actgtagtgt tgattgcact
     1381 ggccctggta gggttcattc tgaccgatta ttttgccggt aaatcaagag gtatggtggg
     1441 tggtccctcc aatacggttg gtgtagtaaa tggtaaaaaa attctctttg aggatttcag
     1501 taagaaagtt acacaggccg aagagaacat gaaagcgcag ggttatccac cagctgctgc
     1561 aagccagcag gcactggatc aggcctggtc acaggagatc aaccaggcca ttctgagtga
     1621 tgagtttgaa aagcttggta ttgccgttgg ccggaaagag ctgggtgata ttctttacgg
     1681 acaaaatgca ccgcaggata tcaaacaaca gtttactgat gaaaaaaccg gacagtttaa
     1741 tgctgcgctt gccaagcaga acatagatca gaaactgaaa agcggtacag cggaagaaaa
     1801 agcaaatatc aacgcctata tcaatcagtt ggtgaactta cgcatggcgg ataaattttt
     1861 ctctctgatg tctggtacaa tcaactttcc taaatggatg attgaaaaac aaaatgcgga
     1921 aaatgcacaa atggcccgca tttcactggt aagagaaaca tacgccagta ttcctgacag
     1981 cagtattaaa gtagaagaca gcgagattga agcgtttgtc aacaagaata aaaaactttt
     2041 caaacaggca gaaacccgga gcatcagctt tgtaagtttc agcgctgcgc cttctgcaaa
     2101 agatagtgcc gctacgctta gtagcttagt tacacttcgg gaccgtttcg cttcttcaga
     2161 tagtgttgct cagtttctgg ccggagaggg tgcccagtat tttgatgcat atgtaagcgg
     2221 aaatgcgatt cagattccgg ttaaggattc catctttaaa ttacccgttg gtggggttta
     2281 cggaccatat ctcgatgcaa atagttatgt actggctaaa atgatcggtg taagagtatt
     2341 gcctgattct gttaaatgcc gccacgtatt ggtaagtaat aatctccagc agggtggtgt
     2401 ggatgacagt attgcttccc gcaggatcga tagtattaaa ttagcgatta acggcggtgc
     2461 taactgggat tccatggtgc agaaatataa tccgcccagc gatgccagcc gccagaacaa
     2521 aggtgagatg acgttttcct ctactgaaat tcagggtccg aactttgccc gggaattcgg
     2581 acagttcata ttacttgacg gtaaagttgg cgaacgtaaa gtggtaaaaa cctcttttgg
     2641 ttatcatttt atcgaaatca tgtctgccat caaaccgtct acccattaca aagtggccta
     2701 cctgcctaag gaaatcattg ccagccagga gacagataat gctgcattga atgaagccaa
     2761 ttcctttgct gcagaaagca ccgatttaaa aagttttgat gatacctatg aaaagaaact
     2821 gaaagcaaaa ggtttccaga aaggtattgc gaccgatatt cttcgggcca gcacatctat
     2881 ccgtggtctt gaaaacgctt cccgttcttt tgtaagagat atatatgcgg caaaaagagg
     2941 cgaggtattg aaaccggaac gcgtggataa cagttatgtg gtagccgttg taacagaagc
     3001 actggaagag gggacaatga gtaccgcccg tgcccgtgca gctggtgcag aaactgaagt
     3061 gcgagttaaa aagaaaggcg aactgctgaa aaagaaaatc ggcacagtta ccacactgga
     3121 agacgcaatt gcgaaactgg gtggtcttaa gcagattgag ccgattgaca gcctgcgtat
     3181 ttacggctca aacctgtcat atgaacccaa agtaatcggg gctgccttta atccggccaa
     3241 taaaggaaag gttgttcctg aagtactggt tggaaaacaa ggtgtttatg tgataagggt
     3301 ggaagaccag agcagtacag cagttccggc tgaagatatc aatgccgtaa gactttcacg
     3361 ttaccagcag gccaagcaaa gcctgggtaa tcaatatagt cagaacaacc ccctcactat
     3421 cctgcggaat gcagcggata tcaaggacaa gcgttctgaa cgtttctaag cgaattcgtt
     3481 aactgatcgt tgaaccccgg aaccctaaac gttccggggt tttttgtttt ccagatttcg
     3541 caaccagggc gcggctcaat ttgttatttt tacataccga aagaatatga gcgaaccttt
     3601 tgtaaataaa gtggctgaca gtggcctgat caccctggac ctcgagaaat ggtaccccag
     3661 ggaagagaca gctgtttttg atattaagga ttacctgttc atgggcatga ttctgaagga
     3721 aaaggatttc agggaagcgc tgaaagcaaa ggattggaca atttatcagg acaaaaatgt
     3781 ggcgctgacc tgcagcgtgg atgccatcat tcccgtgtgg gcctatatgc tggtagccgc
     3841 caacctgcag ccctttgcga atgaaattgt gatgggtgat gaaaaggaac tacataaaac
     3901 cattttcctg aagaatcttt ctgcaattga cgtgcaggag ttttatgata aacgcgtggt
     3961 gattaaagga tgtggcgaaa cacctattgc ggattatgtc tacatggaga tcacgaaact
     4021 tcttcgtccg gtggctaaaa gcattatgta tggcgaaccc tgcagtacgg taccggtgtt
     4081 taaaaagcag gggggcccaa agaaagatta gctttttttt attcttcctt ccaatacggg
     4141 cttatattcc ttaaatgttt taagatagct gatcatggcc tggacgattt cgagggttgt
     4201 tttctttctg gatacagcct ttttaaatgc caggaaacca tcgccgccat cagccagaaa
     4261 attggaagta agtactttat aaattttatt ccgattcagt tcttctgtat ttaatttaca
     4321 actggttatt cgtgaaccaa caggaagggt ttcattataa acgatttcaa ctcctttcga
     4381 cacctgcaat atgccgttgg ttagtgcggc gccatgctcg aacaactcat acaaatcagc
     4441 ccccgtcagc tctaactgat ggatagtgtt tggaaaaggg aaggcagata ttatatttcc
     4501 tgtggttacg gttcccgcct caatatcctg tcttaaccca ccactattag taacggccag
     4561 atcaaagccc ggataagcat acagaatagc atctgccacc atattcccca ttaaagattc
     4621 ttcgccatat gaacgggtaa gcgcaaacgg aattgttgta acctcttctt cggtaatttt
     4681 ttttactttt tctttccata caagaattgc ttttgctgta gcagagtcat ccgctacttc
     4741 atcatcaaac aggtagccca gggtgttttt atgggcaatg attttatctt taaccggatg
     4801 ataatttatt tcaagcttgc ccaattctga gcccaacgca tcggtggata caataatagt
     4861 tccattggaa agcaaggctt gtggtgtacc ctggtgtgcg tgaccggtaa tcataatatc
     4921 aattcccgga atggccgcag caagatcaat atcccgttgc aggttacggg ccacatcggt
     4981 acttcctgtg gaggattgtc gacccggtat tccctggtgg accaacagga ttatgaggtc
     5041 tgctttttta cggagttctt ttatatattt tttcaaatag atttcttcat ttcttgcttc
     5101 aaccccttgt atcatttcat cggcaatcgt atcgtaaaaa gcaaattttc cgtgcagtcc
     5161 aattacgcca atgcgtatac catccatctg aataatggtg taaggattat tccagatcaa
     5221 ttttttcttt cctttcaaaa agatattccc gtttactacc gggaatcggg ctttcttcaa
     5281 ctgtacaggt acgttttccc agccgtgatc aaattcgtga ttgccgatac acgcggcatc
     5341 gagcgagaga taattcatga catttataac agcctctcct ttggtcaatg agctgataa
//
LOCUS       DAOPMW010000224         5394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17621_length_5394_cov_8.430043, whole genome shotgun sequence.
ACCESSION   DAOPMW010000224 DAOPMW010000000
VERSION     DAOPMW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_5394_cov_8.430043"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="iscX"
                     /locus_tag="PLU11_10560"
     CDS             complement(<1..173)
                     /gene="iscX"
                     /locus_tag="PLU11_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HPN59609.1"
                     /translation="MSTHFEPPINWNDHEDIALKLYERFGDDFTESKIYRIRFTDLLE
                     WVLEIPRFEGTREG"
     gene            complement(276..611)
                     /locus_tag="PLU11_10565"
     CDS             complement(276..611)
                     /locus_tag="PLU11_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN59610.1"
                     /translation="MYTIKFNFEQKDLEPVVLSNIESGQSLLEIVLKNDIELHHNCGG
                     VCACTTCHLYVEKGMEYIDELSDREEDFIDRAMNPRLNSRLGCQSLLLDGSGEIEVTL
                     PDQTQFLGE"
     gene            complement(684..1445)
                     /locus_tag="PLU11_10570"
     CDS             complement(684..1445)
                     /locus_tag="PLU11_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPN59611.1"
                     /translation="MDIVIIGSGNVAAVLGRKFKAAGHTILQVISRNAKAASELAYEW
                     DTESSNYKSTINRLADIYIIAISDHAIAEMVDDLKLPGKVVAHTAASVPKEVLQNVTE
                     HFGVFYPLQSLRTEMPGLPDIPVYVEGSDELTTTKLKALAQSISPEQVAEADAESRAK
                     LHVAAVLVSNFTNHLYKLAADYCRKEGLDFKQLQPLIEETALRLRSMSPGEAQTGPAI
                     RQDEETIQKHLDLLKNEPGLKEIYEQMTRSIRTTN"
     gene            1637..2497
                     /locus_tag="PLU11_10575"
     CDS             1637..2497
                     /locus_tag="PLU11_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN59612.1"
                     /translation="MKLAQRIALRYFRTKFSLLATISKKKAAEKAFELFCTPQSRNKK
                     ELPPIFKQADPLSFKLDGVIVKGWRWMPESGELPATGRKAMICHGFESSVINFDRYIN
                     PLLKKGYEVLAFDAPAHGRSGGRQINAPLYKKMILEVNKQYGPVHSFMAHSFGGLGLS
                     LAMEEINHTPSHKLVLIAPATESKTAADTFFRFLQLNPSIRQEFDRLIIQKSGLSIEW
                     YSIRRAMKNIRASVLWVHDEDDDTTPLSDALKVKEDGNENVEFMITKGLGHRRIYREN
                     KVTKAIIDFL"
     gene            2608..3213
                     /locus_tag="PLU11_10580"
     CDS             2608..3213
                     /locus_tag="PLU11_10580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN59613.1"
                     /translation="MNNYTYLALGDSYTIGEQVPFADNYPNQAVQLLRKKGVDFHAPE
                     IVARTGWTTDELQFGINNRLFRSSYDFVTLLIGVNNQYRGRSVEEYEEQFTVLLEQAL
                     AFAGNRKERVAVLSIPDWGITPFAATREGGKIAEEIDRFNEVNQRVSTAAGIHYIYIT
                     GLTREAAQDASLLAPDGLHPSGKEYQRWAEKVAAFFSNIIS"
     gene            3392..>5394
                     /gene="topA"
                     /locus_tag="PLU11_10585"
     CDS             3392..>5394
                     /gene="topA"
                     /locus_tag="PLU11_10585"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPN59614.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGKDFQVKSCFGHIRDLEKAGMGI
                     NLEKNFEPSYIVPADKERVVNELKSLAKKSDEVWLATDEDREGEAISWHLCEVLGLDP
                     KTTKRIVFHEITKPAIQAAVQNPRLLDMNLVMAQQARRILDRIVGFELSPVLWRKMSM
                     RNNLSAGRVQSVAVRLIAEREREINAFIPVSTFKIEAAFTAKDVTDKNVTFTAEGKKQ
                     NNPEDAESFLKSCIGATYKVNDIQVKPGKRSPAPPFTTSTLQQEASRKLGYGVSRTMQ
                     IAQRLYENGYITYMRTDSLNLSNTALGDITNTVKSMYGEEYHQFRKFKNKNDSAQEAH
                     EAIRPTYMSNSTVPEPEWLRLYELIWKRTMACQMADAELEKTTAKIEISTNKEELTAS
                     GEVLKFDGFLKVYREDKDEEELEEEANEGMLPPLSVGQQLPFRDMKATERFSRPLPRY
                     TEASLVKKMEELGIGRPSTYAPTISTILKRGYVEKRDKEGTRRDFQVFQLKKDEVTKL
                     MEQENTGAEKSKLFPSDLGLVVTDFLKQYFDDIMDYSFTARIEEEFDEVAEGKMKWNK
                     MILDFYDPFKKDVDNTIENAERIKGERVLGVDPESGKPVVSRMGRFGPMIQIGEANDE
                     EKPRFAKIPTGQSIETITFEEAMNLFGAQGSMGLYEEKEVSVNV"
BASE COUNT         1529 a         1228 c         1284 g         1353 t
ORIGIN      
        1 ccttcccggg tgccttcaaa acgggggatt tcaagcaccc attccagcag gtcggtaaaa
       61 cggatacggt agatcttgct ctccgtaaaa tcatccccga accgctcata cagtttcagt
      121 gcgatatctt cgtgatcatt ccagttaatt ggtggctcga agtgagtaga catgtttaaa
      181 tgatattcgt gattatgtaa ttctaataaa aagatccaag atgccaagat gccaattaaa
      241 aatcaaagca taaataccaa accgttctca gtgagttatt cacccaggaa ctgcgtctgg
      301 tcaggcaatg tgacttcgat ctcaccgctt ccgtcgagta agagggattg acaacctaat
      361 cttgaattca gccggggatt catagcccgg tcgataaaat cttcctcccg gtctgagagt
      421 tcgtcaatat actccatgcc tttttccaca taaagatgac aggtggtaca ggcacaaacc
      481 ccgccacaat tgtggtgaag ctcaatatcg tttttcagga cgatctcgag gagggactgg
      541 cctgactcta tattggaaag tacaaccggt tccaggtcct tctgctcaaa attgaattta
      601 atcgtgtaca tggtattgtt ttcgtttcgg ggagcaaatt tacctcaaaa acacggaaac
      661 ggtttacttg ttcgggaaaa caatcagttt gtggtccgga tactccgggt catttgttca
      721 taaatttcct tcaaacccgg ttcattcttc aataaatcca ggtgtttttg gatagtttcc
      781 tcatcctggc ggatagcggg accggtctgg gcttctccgg gactcatact ccgtagccgg
      841 agggctgttt cttctatcag gggctggagt tgtttgaaat ccaggccctc tttccggcaa
      901 taatccgccg cgagtttata cagatgattg gtaaaattgc tgaccagcac tgccgccaca
      961 tgcagtttgg cccggctttc tgcatcagcc tccgctactt gttcggggga aatggattgc
     1021 gccaatgctt ttaatttagt cgtagtcagt tcatcactcc cttctacata aacagggata
     1081 tcaggtaaac ccggcatttc ggtacgcagg ctctgcaaag gataaaacac gccaaaatgt
     1141 tccgtcacgt tttgcaatac ttcttttgga acagaagcgg ccgtatgcgc caccactttg
     1201 ccgggcagtt tcaggtcatc taccatttcg gctatggcat gatcacttat tgcaattata
     1261 taaatatccg ccagacggtt aatcgtgctt ttataatttg aagattctgt atcccactcg
     1321 taggccagtt cagaggcggc tttcgcgtta cgactgataa cctggaggat agtatgtccc
     1381 gctgctttaa attttctccc caataccgca gctacattgc ctgaaccgat gatgacgatg
     1441 tccatttggt agaatttaga gtaaaggtaa gtaatgttga aaggcggaaa ggttgaaagg
     1501 ttgacaagtt gataggttga taaggaagga gataccgtaa aattcaagaa ataattctaa
     1561 ggaggtcctt gttaactttt caacttgtta acttatcaac ttctgtactt gtcaacttgt
     1621 taactaattt cgtcccatga aactcgccca gcgcattgcc cttcgctatt tcaggactaa
     1681 attcagcctg ctggctacca tctccaaaaa gaaagcggcg gagaaggctt ttgagttgtt
     1741 ctgtactccg caaagccgga ataagaagga gcttccgccc atatttaaac aggcagaccc
     1801 gctttctttt aagctcgatg gagtaatagt gaaaggctgg cgctggatgc cggagagcgg
     1861 cgaattaccg gccactggca gaaaagcaat gatctgccat ggatttgaat cttccgttat
     1921 caattttgac cggtatataa accctttgct gaaaaaagga tatgaagtac tggcatttga
     1981 tgcaccggca catgggcgaa gtggcggcag acaaatcaat gccccgctct ataaaaaaat
     2041 gatcctcgaa gtcaataagc aatacggacc ggtgcattcc tttatggccc attcctttgg
     2101 cggacttggt ctcagcctgg ccatggaaga gatcaatcat accccctccc ataaactggt
     2161 attaattgca cccgctactg aatcaaagac cgccgctgat acgttcttcc gtttcctgca
     2221 attgaatcca tcgatccgtc aggagtttga ccgtcttatt atccaaaaaa gcggattgag
     2281 tattgaatgg tactccatcc gccgggcgat gaaaaatatc agggcaagcg tgctctgggt
     2341 acatgatgaa gatgatgata ccacgcccct ctccgatgcc ctgaaagtaa aagaagatgg
     2401 caatgagaat gttgaattca tgatcaccaa aggactcggt caccgccgga tctaccggga
     2461 gaataaagtc accaaagcga tcattgactt cctctaacca ctcaccactc accactcact
     2521 actcactact caccattcac cattcaccaa tttcccttaa ttttcaacct gcctgccggt
     2581 aggcaggtat tcaatgctca attttcaatg aacaattata cctacctcgc tcttggcgat
     2641 tcgtatacga tcggagaaca ggttccgttt gcggataatt atcccaacca ggccgtgcag
     2701 ttgctccgga aaaaaggggt tgattttcac gcccccgaaa tagtggcccg gaccgggtgg
     2761 accaccgatg aactgcaatt tggtatcaat aaccggcttt tcaggtcttc ctatgatttt
     2821 gtgaccctgc tgatcggggt aaataaccag taccggggac gttccgtgga ggaatatgaa
     2881 gagcagttta ccgttttgct ggaacaggcc ctggcctttg ccggaaaccg gaaagagcgg
     2941 gtggcggttc tctctattcc tgattgggga atcacccctt ttgccgctac cagggaaggc
     3001 gggaaaatcg ctgaagaaat tgaccgtttt aatgaggtca accagcgggt ttccaccgct
     3061 gccggcatac attatatata tattaccgga ctgacccgcg aagctgccca ggacgcttca
     3121 ttactggctc cggatggttt acatccgtcg ggaaaagagt atcagcgctg ggcggagaaa
     3181 gtcgctgctt ttttttcgaa cataattagc tgattatctg ctagttgaaa gctacctatg
     3241 aaagcaataa aatgacttgt caggcgacga taaaaaatct ttcgggaaaa atttattatc
     3301 cttgtcctaa cttgcggccg ctgaaaggcg gttttttcgt aaaagtcagc cggaaaaaaa
     3361 ggcgaaagac cgataaaata cagtatccct tatggcgaaa aatttactga tagtagagag
     3421 ccctgcgaaa gctaaaacga ttgagaagat cctgggaaaa gacttccagg tgaagagttg
     3481 tttcggccat atccgtgacc tcgaaaaggc cggtatgggt atcaatctcg agaaaaattt
     3541 cgaacccagc tatatcgttc ctgcggataa agaacgcgtt gtgaatgagt taaaatcact
     3601 ggccaaaaaa tcagacgaag tatggcttgc aacggatgag gaccgtgagg gggaagccat
     3661 cagctggcat ttatgcgagg tactcggcct cgatcccaaa accactaaac ggattgtatt
     3721 ccacgagatc actaaacccg ccattcaggc agcagtacag aatccgcgtt tactcgatat
     3781 gaacctggta atggctcagc aggcccgccg gatacttgac cggattgttg gatttgagct
     3841 cagtccggta ctctggcgca aaatgagtat gcggaataat ctcagtgccg ggcgtgtaca
     3901 gagtgtggcg gtacgactga ttgctgagag ggaaagagaa atcaacgcat ttattccggt
     3961 cagtactttc aaaatcgaag ccgcattcac tgcaaaagat gtaacggata aaaatgtaac
     4021 ctttactgct gaaggtaaaa aacagaacaa tccggaagat gcagaaagct ttttgaaaag
     4081 ctgtatcggt gctacttata aagtaaatga tattcaggtg aagccgggca aacgttctcc
     4141 cgccccgcct ttcactacct ctactctgca acaggaagcc tcccgtaaat taggatatgg
     4201 tgtatcccgt accatgcaga tcgcccagcg gttgtatgaa aacggatata tcacctatat
     4261 gcgtaccgac agtctgaacc tgagtaatac cgccctgggc gatatcacca atacggttaa
     4321 aagcatgtac ggggaagaat accaccagtt ccgtaaattc aaaaacaaaa atgacagtgc
     4381 ccaggaagcg cacgaagcga ttcgtccgac ctatatgagc aacagcacgg tgccggaacc
     4441 ggaatggtta cgtctttatg aactgatctg gaaacggacc atggcctgcc agatggcaga
     4501 tgctgaactg gaaaaaacca cggcgaaaat tgaaatttcc accaataaag aagaactcac
     4561 ggccagtgga gaagtattaa aatttgacgg tttcttaaaa gtataccggg aagataaaga
     4621 cgaagaggaa ctggaagaag aagccaacga aggcatgttg ccaccgctga gtgtgggaca
     4681 gcaattacct ttccgcgaca tgaaagctac ggaacgtttc agtcgtcccc tgccccgtta
     4741 tacggaggct tctctcgtaa agaaaatgga ggaactcggc atcggccgtc cttccaccta
     4801 tgctcccacc atttccacca ttcttaaaag aggttatgtg gagaagcggg ataaagaagg
     4861 aacccgtcgt gatttccagg tgttccagtt gaagaaagat gaagttacca aactgatgga
     4921 gcaggaaaat accggggcgg agaaatccaa actgtttcct tccgatctgg gcctggtagt
     4981 gaccgacttt ctgaaacagt attttgatga tatcatggat tacagtttca cggcccgtat
     5041 tgaagaagag tttgacgaag tggcggaggg caagatgaaa tggaataaaa tgattcttga
     5101 tttctacgat ccatttaaaa aggacgtgga taatacgatt gaaaatgcgg aacgcattaa
     5161 aggggaacgg gtactcggtg tggatccgga gagcggcaag ccggtggtat cccgtatggg
     5221 tcgttttgga ccgatgatcc agattggtga agccaatgat gaggaaaagc cccgctttgc
     5281 caaaattccg accggacaaa gtattgagac gatcacgttt gaagaagcca tgaacctttt
     5341 tggcgcacag ggttccatgg gattatatga agagaaagaa gtgtccgtaa atgt
//
LOCUS       DAOPMW010000225         5383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17677_length_5383_cov_6.316817, whole genome shotgun sequence.
ACCESSION   DAOPMW010000225 DAOPMW010000000
VERSION     DAOPMW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17677_length_5383_cov_6.316817"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLU11_10590"
     CDS             complement(<1..1035)
                     /locus_tag="PLU11_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN59615.1"
                     /translation="MRLKSLEIKGFKSFADKTVVNFDDNITGIVGPNGCGKSNIVDSI
                     RWVIGEQKISQLRSENLDSLVFNGSKTRSASGLAEVSLTFENTRNLLPTEFSTVTVTR
                     KFYKSGESEYRLNDVQCRLKDIQNLFMDTGISTDSYAIIALDMVDDLIKDKDNSRRKM
                     LEQAAGISIYKTRKKEARQKLEATESDLARIEDLLFEINNQLKALESQAKKAEKYHEI
                     KKDYREISIELAKASLEGFNLSYKELNEQSEVETDKKHRLEAEIGTREAELEQEKLAF
                     VERERQLQSMQHQFNEMLQTVRSKENDKNLSAQRLEHLHEKEGSLGEFLQKAEGQLKG
                     IEESIGFTSLQ"
     gene            1272..1997
                     /locus_tag="PLU11_10595"
     CDS             1272..1997
                     /locus_tag="PLU11_10595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN59616.1"
                     /translation="MKYWNPLLWLILLLPFGCNPAKRLTGPKVVVVWQPSHQTDTGKD
                     FSEAATCNAIVEAAMATQSGRKEYKVWSLGKTEYHHADSGSNTKIQHTSALIEGKISG
                     YAYELQESNKLKPRVFISVHNNGGTRRNAVWGYIHYGDKYEADNRELAAHLVKAICEV
                     SGMENRGVLLDSTTGRNDYRCAATGKLSFYSLDEQVNTAPYRVLLEIGDNAASRAILT
                     DPAKQKLIGEAIKKALVEWMNTK"
     gene            2260..3075
                     /locus_tag="PLU11_10600"
     CDS             2260..3075
                     /locus_tag="PLU11_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HPN59617.1"
                     /translation="MKKIIAAVLCLFLITDGSAQSGDWDTYLARYEKGPGSTLVNMSL
                     KQKAPDSTRPFLFAAGVKFKNCSADGLPAPTAFITLNRISDSIVALVGRHPGSLLTGT
                     FSYQCERKDYFYVRDTAGLKSAVTRLLTDFFPGYTAAFLIKEDRTWDAYLHFLYPNEE
                     TRLFMYNQAIVMRLEKSGDQTAIPRPIDHILFFKTEKDRECFIYKAISQQYKIVIKDS
                     TDRKEGSYRLQITRNDKADLATVNKITGWIKKEATRCKGEYEGWETVIRQLPG"
     gene            3226..4272
                     /locus_tag="PLU11_10605"
     CDS             3226..4272
                     /locus_tag="PLU11_10605"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPN59618.1"
                     /translation="MSDSGTFTADDEESANASTKRRTLQPYPAMLAYWDKDELCRFVN
                     NAGLEWFNQKSESVIGKMSIRDFVGQDLYNENRYYIRSALSGHPHVFERSLKAVDKRE
                     ISAVVTFIPDTNKGETVGFYSHVADVGHLKKNSDARLADEKELLRAIIDIQEKERSQI
                     SEILRDNVNQLLVYVNLMMQGKKDNPEQGAFSEDMKLAIHQAVLELNKLSNQLYPSAL
                     SLLGLLPSIENLISNYRQHGAPDISFYCNEPQIEELSHKDKLSVYRIVQDFIGLILNR
                     RKSSYIITELSYRDFCLMIRIVYNGEGPTIDHRSDEFRDIRSRIDYYSGKIREFHRED
                     EKVFIAHLVFHPQD"
     gene            complement(4273..>5383)
                     /locus_tag="PLU11_10610"
     CDS             complement(4273..>5383)
                     /locus_tag="PLU11_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59619.1"
                     /translation="LINAVTDEHIWADSYDRPMLDIFSIQREVALAIAAVLNTSLSNK
                     ETRELDYIPTTDLLAYDYYLRGKLLLENRNKADVLLARDYFIQAVNRDKTFAIAYSGL
                     ADTYLLSSFRGYEDPVMMLWQAKKYIDTAASLDSFSGETQASLGYWYFQSFDWHAAEI
                     TYRRSISLNPNQTNVYLWLAILLEAKGENEEALKIFEMGAEVNPMWDYLQKNKILLLV
                     NMNRKEEAFACMKGLITKSHDSSVKRKRYEELSRLHWAYNEKTEAIAAARKSGNLPLL
                     KFYTDGDYSALQKMVDDHYEGLRTRSEYVSQLWMGLDYAHAGAREKALDCFNNAVALK
                     EAAVSLLLIRDFEYLNIKYLSMALLTRKVKQLINF"
BASE COUNT         1423 a         1257 c         1226 g         1477 t
ORIGIN      
        1 ctggagtgag gtaaagccaa tactttcctc tatccctttc agttgtcctt ctgctttctg
       61 caggaattca ccgagacttc cttctttttc atgcaggtgt tccaggcgtt gggcagaaag
      121 gttcttatca ttttctttac tgcggacagt ctgcaacatt tcgttgaact ggtgctgcat
      181 cgactgtagt tgtctttcac gctctacaaa tgccagcttc tcctgttcca gttccgcttc
      241 gcgggtacca atttccgctt cgagacggtg tttcttgtcc gtctccactt cactttgttc
      301 attcagttct ttatacgaaa gattgaatcc ttccagcgat gccttggcca gctcaatact
      361 gatctcgcgg taatcctttt tgatctcatg gtatttctcg gctttcttcg cctggctttc
      421 cagcgctttg agctgattgt tgatttcaaa aagaaggtct tcaatacgcg ccaggtcaga
      481 ttcggttgct tccagtttct gccgggcttc ttttttccgg gttttgtaga tggagatgcc
      541 agccgcttgt tccagcattt tgcgccggct gttatccttg tccttgatca ggtcatctac
      601 catgtcgagg gcgatgatgg cataactgtc cgtgctgata ccagtatcca taaacaggtt
      661 ctggatatct ttcagacggc actgtacatc attcaggcgg tattcgctct ccccgctttt
      721 ataaaattta cgggtaaccg taaccgtgct gaattcagtc ggtaacaggt tgcgggtatt
      781 ttcaaatgta aggcttacct cggccagtcc gctggcacta cgggtctttg atccgttgaa
      841 caccaggctg tccagatttt cactccggag ctgactgatt ttttgttcac cgattaccca
      901 acggatactg tccacgatat tcgatttacc acatccgttc ggtccgacga taccggtgat
      961 attgtcgtca aaattcacca ccgtcttgtc ggcaaagctc ttaaagccct tgatttctaa
     1021 actctttaat ctcacggttt actttttaat ttcgggttaa gtcaggcggc gaatttaagc
     1081 caaagggaga taaaatatgg gacgctgtgg gggcagttat tcacaataag gatatgaaaa
     1141 tgtgggaaga ttttttaagg attggcccca tggacaggaa tcagacccag cccaaaaaag
     1201 gtcaatataa accagcactt tatcaggaca gaaaagcggg caattgtcct acttttagct
     1261 aaagaaccga tatgaaatac tggaaccccc tgctttggct gatcctttta ctaccctttg
     1321 gttgcaaccc ggctaaaagg ctaactggtc ctaaagtggt tgtagtctgg caaccctccc
     1381 accagaccga caccggcaag gatttcagtg aagcagcaac ctgtaatgct atagtggaag
     1441 cagcgatggc tacccaatcc ggacggaaag aatataaagt ctggagcctg ggaaaaacgg
     1501 aataccacca tgccgactct ggcagcaata ccaaaatcca gcatacttcg gctcttattg
     1561 aggggaaaat atccggttat gcttatgaat tacaggaatc aaataagttg aagccccggg
     1621 ttttcatttc tgtacacaat aacgggggca cccggcggaa tgccgtatgg ggctatatcc
     1681 attacggaga taagtatgag gcagacaatc gggaactggc agctcacctg gtaaaagcca
     1741 tttgtgaggt ttcgggtatg gaaaaccggg gggtgttgct ggacagcaca accggccgga
     1801 atgactatcg ctgtgccgcc accggcaaac tcagttttta cagcctggat gaacaggtca
     1861 ataccgcccc ctaccgggta ttgctcgaaa taggcgataa tgcggccagc cgggcaatcc
     1921 tcaccgatcc cgcaaaacaa aaactgatcg gggaagcgat caagaaggcg ctggtggaat
     1981 ggatgaatac aaagtaagaa gtacgaggta cgaagtacga ctgacgtgcc ttgaacatat
     2041 aacttatagg aggggctgtt tcctaacacc taacagctaa caacgaacac cttctaaata
     2101 agggctgttt cctgacatct ggcaactggc atctgacaac tttttttcgg gctgttccct
     2161 gacacctgac acctggcatc tgacaaccga tttctaatta aatcataaaa tatcaaccct
     2221 tccccgtgtc ccggcacaat cgggttaact tcgccgggca tgaaaaaaat aatagcagca
     2281 gttttatgct tattcctgat tactgacgga tctgcacagt ccggtgattg ggatacttac
     2341 ctggcgcggt atgaaaaagg accgggcagt accctggtaa atatgtcgtt gaagcaaaaa
     2401 gccccggaca gtacccgtcc ttttttgttt gcggctgggg tgaaatttaa aaactgttcg
     2461 gctgacggat taccggcccc cactgctttt atcacattga acagaatttc agactctata
     2521 gtagcattag ttggccgcca tcccggcagt ttgctgaccg gcactttctc ctaccaatgt
     2581 gaacgtaagg attattttta tgtccgtgat actgccggac tgaaaagtgc tgtaacccgg
     2641 ctgctgactg atttttttcc gggttatacg gccgctttcc tgatcaagga agacagaacc
     2701 tgggatgctt atctccattt cctttatccc aatgaggaga cccggttatt tatgtacaat
     2761 caggcaatcg taatgcgatt ggaaaagtcc ggagatcaaa ccgctatacc cagacccatt
     2821 gaccacatcc tgttttttaa gacggagaaa gaccgggagt gttttatcta caaagcgatc
     2881 agtcagcaat acaaaattgt tatcaaagac agtacggacc ggaaagaggg atcttatcgt
     2941 ttacaaatta cccggaacga taaagccgat ctcgcaacag tcaataaaat caccggttgg
     3001 ataaaaaagg aagccacccg atgcaaaggc gagtatgaag gatgggaaac tgtgatccgg
     3061 caattacccg gttaaaaaca cttatttttc atttacccct actatttttt gtaaaaaaat
     3121 agtcatatct gcttagtgaa cccggatttt acctttcctg gtacaggggt tttacatttt
     3181 gtttaccctt ctttttttac taaattacag gtaacaaatc gcgctatgtc agacagtgga
     3241 acttttactg ctgatgacga agaatcagcc aatgcttcaa ccaaaagaag aactctccag
     3301 ccttatccgg ccatgctggc atactgggat aaagatgaat tatgccggtt tgtaaataat
     3361 gccgggctgg aatggtttaa tcagaaatct gaatcagtaa tcgggaagat gagtatccgc
     3421 gattttgtgg gacaggatct ttacaatgaa aaccggtatt atatccgctc cgctttatcc
     3481 ggacatccgc atgtatttga acggtcatta aaggcggttg acaaaaggga gatttcagcc
     3541 gtggttactt ttattccgga taccaacaaa ggggaaacgg tgggcttcta ttcgcatgta
     3601 gcggatgtag gacatctgaa aaaaaattcg gacgccagac tggccgatga aaaagaatta
     3661 ctccgggcca ttattgatat ccaggaaaaa gaaaggtcgc agatttctga aatcttacgg
     3721 gacaatgtca accagctgct ggtatatgtc aacctgatga tgcagggaaa aaaagacaac
     3781 ccggaacagg gtgctttttc agaagacatg aaactcgcca tccatcaggc cgtcctggaa
     3841 ctgaataaac tgagcaatca gctctaccct tctgctttgt cgctgctcgg tcttttaccc
     3901 agtattgaga atctcattag taattaccgt cagcacggcg caccggatat cagtttttat
     3961 tgcaacgaac cacagattga agaactcagt cataaggaca agttatcggt gtaccggatt
     4021 gtccaggatt ttattggact tatactgaac cgccgtaaaa gcagctatat catcaccgag
     4081 ctcagttacc gggatttttg cctgatgatc cgcattgttt ataacgggga aggccccaca
     4141 atcgatcacc gaagtgatga attcagggat atccgttccc ggatcgatta ttactctgga
     4201 aaaatcaggg aatttcaccg ggaagatgag aaggtattta tcgcccacct ggtgtttcat
     4261 ccacaggact gatcagaaat tgatcagttg tttgacttta cgggtaagca gggccatgct
     4321 caggtattta atattcaggt attcaaaatc acggatcagt aatagtgaaa cggcggcttc
     4381 tttgagggct accgcattat taaaacaatc cagggctttt tcccgggcac cggcatgggc
     4441 ataatccagt cccatccata gttgtgatac atattcactc cgggtgcgga gtccttcata
     4501 gtgatcatcc accattttct gcagggcgga ataatccccg tcggtataga atttaagcaa
     4561 aggcaggttg cctgatttcc gggcggctgc tattgcttcg gttttctcat tatatgccca
     4621 gtgcagcctt gataattcct cataccgctt tcttttcacg ctgctgtcat gcgatttagt
     4681 gatcagcccc ttcatacagg caaaagcttc ctccttcctg ttcatgttga caagaaggag
     4741 aatcttgttt ttttgtaaat aatcccacat gggattcact tctgctccca tttcaaatat
     4801 cttgagggct tcctcatttt ccccttttgc ttccaggagg attgccagcc agagatatac
     4861 attggtctga ttcggattga ggctgatgga acgacggtag gtgatttccg ctgcatgcca
     4921 gtcaaaactc tggaaatacc agtaacctaa agaagcctgt gtttcgcccg aaaatgagtc
     4981 caggcttgca gcggtatcaa tatacttttt ggcctgccag agcatcatta ccggatcttc
     5041 atatccccgg aaagaagaca acaggtaggt atccgccaat ccggaataag caatagcaaa
     5101 ggttttgtca cggtttacgg cctggataaa gtaatcccgg gccaggagta catcggcctt
     5161 attcctgttt tccagcagca atttaccccg gaggtaatag tcatatgcca gcaggtccgt
     5221 ggttggtata taatcaagtt cccgggtttc cttgtttgaa agactcgtgt tcaacaccgc
     5281 tgcaatcgcc agggccactt cccgttgtat agaaaagatg tccagcatcg gtcggtcata
     5341 tgaatctgcc cagatatgct cgtccgtaac tgcattgatc agc
//
LOCUS       DAOPMW010000226         5372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17739_length_5372_cov_7.589994, whole genome shotgun sequence.
ACCESSION   DAOPMW010000226 DAOPMW010000000
VERSION     DAOPMW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17739_length_5372_cov_7.589994"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PLU11_10615"
     CDS             <1..1511
                     /locus_tag="PLU11_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59620.1"
                     /translation="TVINNLVQGVYVFRLTATDNNGATADAFVTITVNAAPNLPPVAS
                     AGSDRIITLPTNSLTLTGSGSDNDGTIAAYQWNKISGPAQFVILTPTQAQTLVNNLVQ
                     GTYQFELRVTDNQGAVGRDTVTVIVNPAAPSNQPPVANAGSNLTITLPVNNVTLFGSG
                     TDSDGLIATYQWTKIAGPATFIIATAAQAQTAINNLVQGTYQFELTVTDNLGASGKDT
                     VTVTVNPAIAVNQAPIVNAGNDQSISLPVNTVNVLGSATDTDGTIAAYQWTKIAGPAS
                     FTIVFPTQAQTTINNLMEGTYQFELRATDNLGAIGRDTVTIIVTSAPNLAPSANAGPD
                     RSMTLPTNSITHTGSANDADGTIASYLWTKIAGPARFTIATPDQVQTTFSDLEEGVYQ
                     FELAVTDNLGAIDRDTVTVTVKGSGSLLKQVYPNPATDLINIKVNGTNARTSLVMHIY
                     DSRGVSVYNKVLPAGQPLLTETVELSKFSNGIYIIEVMADGANRSTMKFVKQ"
     gene            complement(1777..1842)
                     /locus_tag="PLU11_10620"
     tRNA            complement(1777..1842)
                     /locus_tag="PLU11_10620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1792..1794),aa:Ser,seq:cga)
     gene            complement(1957..2079)
                     /locus_tag="PLU11_10625"
     CDS             complement(1957..2079)
                     /locus_tag="PLU11_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPN59621.1"
                     /translation="MPFTVYIIYSKIIDQYYIGHTENLDDRLYRHRNGGSLSTK"
     gene            complement(2115..2188)
                     /locus_tag="PLU11_10630"
     tRNA            complement(2115..2188)
                     /locus_tag="PLU11_10630"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ala,seq:ggc)
     assembly_gap    2290..2389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2504..3562)
                     /locus_tag="PLU11_10635"
     CDS             complement(2504..3562)
                     /locus_tag="PLU11_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN59622.1"
                     /translation="MKFEAVTIKDIAKALGLSTSTVSRALRDSYEISPETKKVVLEYA
                     EKLNYHPNPIALSLKERRSRSIGVIVCEIANSYFSQVINGIESIANTNGYNVIISQSH
                     ESAEKELVNLQYLTSRSVDGLIISVSTETKDFNYFNELHDRGMPIVFFDRIVDEIQTH
                     KVIADNFKGAYDATTHLINSGYKRIATLCNTVFLSIGKERLAGYQAALKDHGLEPDES
                     LIRYCQHGGMVAEEMDQAMDELFTLQNPPDAIFAAADKLTTGCLRALRAKGLSVPEDV
                     GLIGFSNSELTELLNPSLSVIRQPAFEMGEVATSLLLELIESKRPVTDFQSRVLTTEM
                     ILRSSTRMQKTHSKPVIS"
     gene            4076..>5372
                     /locus_tag="PLU11_10640"
     CDS             4076..>5372
                     /locus_tag="PLU11_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN59623.1"
                     /translation="MNRRFQLKGLFMLLFALIPLLGLMAQTRPVTGTVVSSENKQPLQ
                     GVTVTVKGTRTGTTTDANGNFKINAGENDVLIFSNVSFTTLEVTVGKNSNLSVTLISD
                     AKAMDDVVVIGYQTVRRKDLLASVSSVGSKDLKDIPINSAAEALNGRLAGVTATTSEG
                     SPDAEIRIRVRGGMSITGDNSPLYIVDGVQVENGLSTISPQDIQSIDVLKDAAATAIY
                     GARGANGVIVITTKSGKPGRLIISYNGFVGVKELAKRLDVFNPYDFVKYQWERSRSSS
                     TDSASFARSFGTTWDTLQNYKNVDAVDWQGETFGRTGISTTHNLSASGGSKKLTYNFG
                     YTYNNDKAIVINSDYKRHLFNAKGEYKINKQLKIGAGARYTHQEVQGAGVSDTKGSSY
                     NRLRNAVKYRPFLSDAQDIDDSDPLADQNVGNGLNLYNPV"
BASE COUNT         1459 a         1235 c         1175 g         1403 t
ORIGIN      
        1 aaaccgtgat caataacctg gtacagggtg tatatgtatt ccgcctgact gctactgata
       61 acaatggcgc aacagccgat gcatttgtaa cgatcaccgt caatgcagct cctaacctgc
      121 caccggtggc cagtgccggt tccgacagga tcattacttt acccactaat agcttaacct
      181 taaccggcag cggttcagat aatgatggca cgatcgccgc ttatcagtgg aataaaattt
      241 caggtccggc ccagtttgtg atcctgacac caacacaggc gcagacactg gtgaataacc
      301 tggtacaagg cacttatcaa tttgaattaa gagtaacaga taaccagggt gcagttggaa
      361 gagatacggt aacggtcatt gtaaaccccg ccgctccatc caaccagcct ccggtagcga
      421 atgctggcag caacctgacc atcaccctgc cggtgaataa tgtaactctt ttcggaagtg
      481 gtactgattc ggatggtctg attgccactt atcagtggac aaaaattgcc ggacctgcaa
      541 cctttatcat tgcaacggct gcacaggcac aaacggcaat caataacctg gtgcagggta
      601 cttaccagtt tgagctcacc gttacagata atctcggtgc cagtggaaaa gatacggtaa
      661 ccgtaacggt gaatccggcg atagcagtca accaggcccc gatagtgaat gcaggaaatg
      721 accagtccat ttctttaccg gttaacacgg tgaatgtact tggcagtgct actgatacag
      781 acggtacaat tgccgcttat cagtggacaa aaattgcagg acctgcttcc tttactattg
      841 tattcccgac gcaggcgcag actaccatta ataacctgat ggaaggaaca taccagtttg
      901 agttacgtgc cacggataac ctgggtgcta tcggaaggga tacggtcacc attattgtaa
      961 ccagtgcccc gaacctggct ccttctgcca atgcaggacc cgaccggtct atgaccctgc
     1021 ctactaattc cattacacat acgggtagtg cgaatgatgc agatggaacg atcgccagct
     1081 atctgtggac taaaatcgca ggcccggccc ggtttaccat tgcaacgcct gaccaggtac
     1141 aaaccacctt cagtgatctc gaagaaggcg tgtaccagtt tgaactggca gtaaccgaca
     1201 atcttggtgc aattgaccgg gatacggtaa cggttacagt aaaaggatca ggcagcttat
     1261 taaaacaggt ttacccgaat ccggctacag acctgatcaa tatcaaagtc aatggcacca
     1321 atgcccgtac ctccctggtg atgcatatct atgatagtcg tggggtgagt gtgtataata
     1381 aagtgctgcc ggctggtcaa cccttgctga cagaaacagt tgaattgtca aaattctcca
     1441 atgggatata tattatcgaa gtcatggcag acggggccaa ccgctcaacc atgaagtttg
     1501 taaaacaata aaattcagac cctgagcatt acaaaaggcc gcttcaaaca gaagcggctt
     1561 tttgtttttc catttattca gatcagaaat ttaaaatgaa ttttgcttaa aagtgattga
     1621 tggcatggcc ttattgccat tattaccaac ggggatatag tgttcagtta tgtttttatt
     1681 atggccggtg taagaaaaaa tctatctgtt ttggataaat agtgtaaaca gtaaatggca
     1741 taaaaaaagt catactgttt tttagtatga ctttagtgga gaataccgga gtcgaaccgg
     1801 tgaccttccc gcttgcggcg ggacgctcta tccaacttcc ccgatcagtt gctcaatata
     1861 cttcctgctt tttttagctt taattaccat ttcccgcatg gccgcttcag aacgggtctc
     1921 gaattgctct gtgtacttca actcccagtc ttttgcttat ttggtggaaa ggctaccgcc
     1981 gtttcggtgt ctgtacaggc ggtcatccag attctcagta tgaccgatat aatactggtc
     2041 aatgattttt gaataaatga tatagactgt aaatggcata aaaaaagtca tactgttttt
     2101 tagtatgacc ttagtggaga ataccggagt cgaaccggtg acctcttgca tgccatgcaa
     2161 gcgctctagc caactgagct aattcccctt ccttattttt tgtcgacttt gaccttcccg
     2221 aattccattc gggacgctct agccaactga gctaattccc cttccttatt ttttgtcgac
     2281 tttgaccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccgaattcca
     2401 ttcgggacac tctagccaac tgagctaatt cccctttttt gtttgggagt gcaaatataa
     2461 agggtgaaat caatcgtacc aaaagaaaat gaaatgaaaa aggtcaggaa atcaccggtt
     2521 tagagtgggt tttctgcatg cgggttgaac tccggagaat catttccgtc gtcaagaccc
     2581 gggactggaa atccgttacc ggtcttttac tttcgatcag ctccaataac aggctggtag
     2641 ccacttctcc catttcaaag gcgggttgac ggatgactga aagtgagggg tttaacaact
     2701 cggtcagctc gctgttagaa aaaccgatca gccccacatc ttcgggaacg gataagcctt
     2761 tggcgcggag ggcgcgaaga cagccggtgg taagtttatc ggcagctgca aaaattgcat
     2821 cggggggatt ttggagagtg aataattcat ccatcgcctg gtccatctct tccgccacca
     2881 tacctccatg ctggcaataa cggatcaggg attcatccgg ttccaaccca tgatctttta
     2941 acgcagcctg atacccggcc agcctttcct tacctatgga aagaaacacc gtattgcaca
     3001 aagtggcaat ccgtttatac ccgctgttta tcaaatgagt ggtggcatca taagccccct
     3061 taaaattatc cgctatgact ttatgcgtct gtatttcatc cacaatccgg tcaaaaaaaa
     3121 caatcggcat tccccggtca tggagttcat taaaataatt gaaatctttc gtttccgttg
     3181 agacggaaat aatgagccca tctactgatc gggaagtcag gtattgcagg tttactaatt
     3241 ccttttccgc cgattcatgg ctctgagaga tgattacgtt gtatccatta gtgtttgcaa
     3301 tggactcaat tccgttaatc acctgggaga aatagctgtt ggcaatttca caaacaatga
     3361 ccccaattga acggcttctc cgttctttca gactcagggc aatgggattc gggtgataat
     3421 tgagcttttc ggcatattcc agcactacct tcttggtctc cggactaatc tcataactgt
     3481 cacgcaaggc cctggacact gtggaagtgg acagtccgag cgctttggcg atatctttta
     3541 tagttacagc ttcaaacttc atgtctgttt ccagtggttt ttgcagtcga tgtcagggta
     3601 atcctggccg atatccccaa cagacctgta aattacccta tttgctgcaa attcaaccga
     3661 ttgtccggcg attaccctga aacaggcttt ccacacttta aattttcagt tttcaggcct
     3721 gttttccgga cacgataccg ggaacgattg cgtaaaaata ttgttagtaa tcagggactt
     3781 ataaaaaata acggtgtaga cttgctatgc cgattattga taataacgac agaaaactgc
     3841 ttgccgaatt gaaaagatgt tatgttacca attgatgcta aacaacattt tgagaacgat
     3901 tgtaagaagg tcagtacttc ttttctaatc ccggatacgg catttttctg cactattttc
     3961 ttctcagcat tttattgttc gcttgttaac cgatttcgga cgggcaatta agctggcagg
     4021 gaacggcatt ggaccttatt gtttcttatt ctgcttatca attaaaaact gcattatgaa
     4081 ccgaagattc cagttaaaag gacttttcat gctactgttt gccctgatcc ccctgcttgg
     4141 attaatggca caaacccggc ctgtaacggg tacagtagtc tcctccgaaa acaaacaacc
     4201 attacagggt gttacggtta ccgtaaaggg aacaagaaca ggaaccacta cagacgcaaa
     4261 tggtaatttc aaaattaatg ccggcgaaaa tgatgtactc attttctcca atgtatcttt
     4321 taccacgctc gaagtaacag taggcaaaaa cagcaacctg agtgtcaccc tcatttccga
     4381 cgccaaagcc atggatgatg tggtggtgat cggctaccag accgtacgac gcaaagatct
     4441 gctggcctcc gtatcctctg tgggttcaaa ggatttaaaa gatattccta tcaactccgc
     4501 tgcagaggcc ttaaacggaa ggctggccgg ggtaactgcc accacatccg agggttctcc
     4561 ggatgcagag atcaggatcc gtgtacgggg cggaatgtcc atcactggtg ataattcacc
     4621 actctatatt gtagatggtg tgcaggtgga aaacggactc tccaccatct ctccgcagga
     4681 tatacaatcc attgatgtat taaaagacgc ggctgccacg gctatctatg gtgcaagagg
     4741 cgccaatgga gtaattgtaa ttaccactaa aagcggtaaa cccggacgcc tcattatcag
     4801 ttataatgga tttgtaggag tcaaagaact ggctaaaaga ctggatgttt tcaaccccta
     4861 tgactttgta aaataccaat gggaaagatc ccgcagcagc tccaccgaca gtgccagttt
     4921 tgcccggagt ttcggtacca cctgggatac cctgcagaat tataaaaatg tggacgcggt
     4981 agactggcag ggtgaaacct ttggccgtac cggcatcagc accacgcata acctgagtgc
     5041 atccggtggc agcaagaaac tgacttacaa ttttggctat acctataata atgataaagc
     5101 cattgtgatt aactccgatt acaaacgaca tctctttaat gcaaagggcg aatataaaat
     5161 caacaagcaa ttaaaaatag gagccggcgc ccgctatacc catcaggaag ttcagggtgc
     5221 tggtgtatcc gacacaaaag gatcttccta taaccggctg agaaatgccg tgaagtaccg
     5281 tcccttcctt tccgatgcac aggatattga tgacagtgat ccgctggctg atcagaatgt
     5341 aggtaacggt cttaacctgt ataacccggt gg
//
LOCUS       DAOPMW010000227         5347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_17842_length_5347_cov_7.597128, whole genome shotgun sequence.
ACCESSION   DAOPMW010000227 DAOPMW010000000
VERSION     DAOPMW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17842_length_5347_cov_7.597128"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLU11_10645"
     CDS             <1..361
                     /locus_tag="PLU11_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN59624.1"
                     /translation="ADNYQRYQGIKLVNRFNVISYYRLSQSMDSHDTGRNRGGVKAAL
                     ASIRAKTLVIGIYSDLLYPISEQEFLQQHIPGAELLTITSEFGHDGFLLEYEKIENAL
                     VKFIDERKLSHLKIVNE"
     gene            365..1627
                     /locus_tag="PLU11_10650"
     CDS             365..1627
                     /locus_tag="PLU11_10650"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPN59625.1"
                     /translation="METHKQLTIGLFGFGVVGEGLYKVLQQTPSLKASIKKICIKNIE
                     KKRNAPASLFTTERDELLNDKDINVIVEVIDDADAAFHIVSTALNNGKDVVSASKKMI
                     AEHLTALLELQETTGRSLLYEAAACASIPIIRNLEEYYDNDLLHSIKAIVNGSTNFIL
                     TKMFDEGLDFKQALVLAQQLGFAESNPKLDVEGYDAVNKWTFLLTHAYGITEAPEQIL
                     FNGIQNIQAADAGVAKSKGQAIRLVAQAQKLRNGKVAAFVLPQFINQDDHLAFVKNEY
                     NGVVIESGFADKQFFYGKGAGSFPTASAVLSDIAALRYQYRYEYKKLYHHQPHQLSND
                     IFLRAYVSFDDINLIPKDRFEWIEEWHANEDRKYLVGVISFDELKNNNWWRENNTSLV
                     LTPDPVIEDLELRKLKKKSLELAGIMES"
     gene            complement(1811..2818)
                     /locus_tag="PLU11_10655"
     CDS             complement(1811..2818)
                     /locus_tag="PLU11_10655"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HPN59626.1"
                     /translation="MRKLALSALALMMAMGSLFAKEVDDSTKLLLKQLEAYAKFQDSV
                     KKALKYETGMISLPSGVAKLNVPKGFKFLGLEQSRYVIQDLWGNLPQQHLQGMVFPED
                     GDPFSDSSYAYVVEYNPVGYVKDADAKDINYDDLLKEMKSDDAEENAQRKAMGGEGMY
                     TEGWAAHPFYDDQKKVLHWALDIRVEGRDEHTLNYKVIHLGRKGMMTMSAVSGMGQLD
                     LVKSDVDKVISMAEYTEGNRYSDFDSKVDDVAAWTVGGLVAGKVLLKTAAGAGILKFL
                     KFIIIGIIAAGGAIWKWFSGRRKKEEEFVYEPQPAPVHIEETQLPEPPQATEGTTDKQ
                     I"
     assembly_gap    3090..3189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3330..4277
                     /locus_tag="PLU11_10660"
     CDS             3330..4277
                     /locus_tag="PLU11_10660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN59627.1"
                     /translation="MPELSLVITVKNEEENIAPLLAAVHTALRGMDYEVILVDDGSTD
                     QTKQRIIESADDRTWLVELRKNYGQSNAMMAGIDQAKGTYIALLDGDLQNDPSDIPSM
                     LELLKKEDWDVVAGNRINRQDSMLLRQVPSRIANALIRSFTGVHIKDYGCTLKLFRRE
                     IAAELGLYGELHRFIPVLAKMQGARITQVNVQHHARKYGKGKYGMNRTFKVLSDLVLM
                     VFLRRYMQKPMHLFGTMGFISFGLGLIINLYLLGLKLMGNNIGGKPLLILGLILLLGG
                     IQLITIGIVADINIRTYYESQNKKTYNIRKIYKGKNIIS"
     gene            complement(4297..5289)
                     /locus_tag="PLU11_10665"
     CDS             complement(4297..5289)
                     /locus_tag="PLU11_10665"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN59628.1"
                     /translation="MPQHYTTMHILITGGAGFIGSNLTRKLFQDDPSVRITCIDNFDP
                     FYKEEIKRFNIRDFIALPGYQFIQADISTCSAEEMTGLIKEPVDVIVHIAAKAGVRPS
                     IENPLAYQQANVIGLQQMLDFARLNKIKQFVFASSSSVYGVNDHYPWKEDEQLMPISP
                     YAGTKLAGEMMGHVYSHLFGIRFLALRFFTVYGPGQRPDLAIHKFTKFILQGKPIQMF
                     GDGSTSRDYTFVGDTVKGIIGAIHYTGSDFEIINLGNNYTVSLKELIEAIEDVIGKKA
                     VIEMHPEQPGDVPRTFADVSKAKSLLGYDPQTKLKDGLTAFYDWFNANSELLMK"
BASE COUNT         1452 a         1193 c         1191 g         1411 t
ORIGIN      
        1 tgcggataac taccagcgtt accaggggat caaactggtc aaccgtttta atgtgatcag
       61 ctactaccgg ttgtcgcaga gcatggacag tcatgacacc ggccgtaacc gcggaggggt
      121 gaaagcagcg ttggcttcca tcagggccaa aacactggtg attggtatat actctgatct
      181 cttatatcct atctcggaac aggaattttt gcagcaacat ataccgggcg ccgaactgct
      241 caccatcacc agtgaattcg gacatgacgg attcctgctt gaatacgaga aaattgaaaa
      301 cgccctggtg aaatttatag acgaacgaaa actatctcat ttaaaaattg tgaacgaata
      361 aaatatggaa acacataaac agttaacaat cggattattc ggatttggtg tagtgggcga
      421 aggcttatat aaggtcttac agcaaacccc ttcgctcaaa gccagcatta agaaaatatg
      481 tattaaaaat attgagaaga aacggaatgc ccccgccagc ctgtttacca cggaacgtga
      541 tgaactcctg aatgacaagg acattaatgt aattgtggaa gtgattgatg acgcggatgc
      601 tgcttttcat attgtaagta cagcgctaaa taatggaaaa gatgtggtca gtgccagcaa
      661 aaaaatgatc gccgaacatt taaccgccct gctggaacta caggaaacca ccggccgttc
      721 cctgttgtat gaagcagccg cctgcgcttc catcccgatc atccgcaacc tggaggaata
      781 ttatgacaac gacctgctgc attcgatcaa ggcgattgtt aacggatcta ccaattttat
      841 ccttaccaaa atgtttgatg aaggattgga tttcaaacag gcactggtac tggcccagca
      901 actgggcttt gcggaaagca atcccaaact ggatgtggaa ggatatgatg cggtgaacaa
      961 gtggaccttc ctgctcaccc atgcttatgg cattacggag gcacctgaac agatcctttt
     1021 taatgggata cagaatatcc aggcggctga tgccggggta gccaaatcaa aaggacaggc
     1081 catccggctg gtagcccagg cgcagaaatt acggaacggg aaagtagcgg cctttgtttt
     1141 gccgcagttt attaaccagg atgatcacct cgcttttgtg aaaaatgaat acaacggggt
     1201 ggttattgag agtggttttg ccgataaaca atttttttat ggaaaagggg ccggcagttt
     1261 tccgacggcc tctgccgttt taagtgatat agccgccctg cgataccagt atcggtatga
     1321 gtacaaaaaa ctataccatc atcagcccca tcaactcagc aacgatatct ttctccgggc
     1381 ctatgtcagt ttcgatgata ttaatttaat cccgaaagac cgctttgaat ggattgagga
     1441 atggcatgcc aacgaagacc ggaaatacct ggtaggcgtg atctcctttg atgaactgaa
     1501 aaacaataac tggtggaggg aaaacaatac ttcattagtg ctcacccccg acccggtcat
     1561 tgaagatctg gaattgagaa agctgaagaa gaagagcctg gaactggcgg gaatcatgga
     1621 gtcttaaaaa ggataatggt ttaaagaagg gtttaaaagg tttggaggac taaaggagaa
     1681 agatgtggaa agaagagttt aaaaggttaa aaaatattct cttagaatgg gctgtttcct
     1741 gacaactgac agctgacaac cgacaactat aaaaaaagaa gaggctaccc tttgagtagc
     1801 ctcttctttt ttaaatttgt ttatccgtag ttccttccgt tgcttgcgga ggttcaggca
     1861 gctgtgtttc ttctatatgc accggcgcgg gttgcggttc atagacaaat tcctcttcct
     1921 ttttgcgacg gccggaaaac catttccaga tggcaccacc ggctgcaatg atcccaatga
     1981 ttataaactt caggaatttc aggataccgg caccggctgc tgttttcaac agcactttac
     2041 ccgccaccag gccacccact gtccaggcgg ccacgtcatc cactttagaa tcaaagtcgc
     2101 tgtaacggtt cccctccgta tattctgcca tggagatcac cttatctaca tcggatttaa
     2161 ccaaatcgag ttgtcccatg ccggagactg cactcatggt catcattcct tttcttccca
     2221 aatgaattac tttataatta agggtatgct catcccgtcc ttccacccgg atatccaggg
     2281 cccaatgcag tacttttttc tgatcatcat aaaaaggatg agcagcccat ccttccgtat
     2341 acataccctc accacccatg gctttacgtt gggcattttc ctccgcatca tctgatttca
     2401 tttccttcag cagatcatca taattaatat ccttggcgtc tgcatccttc acatagccaa
     2461 ccggattgta ttcgaccaca taggcatagg aactgtcact gaacgggtct ccgtcttccg
     2521 gaaagaccat accctgcagg tgttgctgcg gcaggttacc ccacagatcc tggatcacat
     2581 accggctttg ttcgagcccc aggaacttga acccttttgg tacattcaat tttgccacac
     2641 ctgacggaag gctgatcata cccgtttcat acttcaatgc tttttttact gaatcctgga
     2701 atttcgcata ggcttccagt tgtttgagca gtagtttagt tgagtcatct acctctttgg
     2761 caaaaaggga acccatggcc atcatcaggg ccagggcgga taaggccagt tttctcatgt
     2821 tgtttgtttt taggtggctg acaaaataat aaaattattt gaaaaaagta ttgatggaag
     2881 tggggaagag tcgggagtca ggagtcggga gtcgggagtc gggagaacag cccctactta
     2941 gaaagttttt ctaaactcta ctttaaacca ctagcttctc tagtactcta taaaccttct
     3001 aaactcttct ttcagtctct ttctcctttc gggctttcta aactctactt taaaccacta
     3061 gcttctctag tactctataa accttctaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnna ctttaaacca ctagcttctc tagtactcta taaaccttct aaactcttct
     3241 ttcaatccct atctcctttc aggctttcta aacccttctt tcaacctctg tgtcctgtaa
     3301 agcatttatc tttacccata tttaccacta tgcccgaatt gtcattggtg attactgtta
     3361 aaaatgaaga ggagaatatt gctcccttgt tggcggctgt gcatacggca ctccggggca
     3421 tggactatga agtgatcctg gtagatgacg gatccacgga tcagacaaaa caacggatta
     3481 ttgaaagtgc tgatgaccgg acctggttgg tcgagctccg gaaaaattat ggacaaagca
     3541 atgccatgat ggccggaatt gatcaggcga aaggaactta tatcgcttta ctggatggtg
     3601 atctgcaaaa tgatccttct gatattccat ccatgctgga attactgaaa aaagaggatt
     3661 gggatgtcgt agccggcaac cggatcaacc ggcaggatag tatgctctta cggcaggtgc
     3721 ccagccggat tgcgaatgcg ctgatccgca gctttaccgg ggtgcatatt aaggattacg
     3781 gatgtacgtt gaaattattc aggcgagaaa tcgccgcaga attgggatta tatggggaat
     3841 tacaccgctt tatcccggtg ctggcgaaaa tgcagggggc ccggattaca caggttaatg
     3901 tgcagcatca tgcccggaag tatggcaaag ggaaatatgg aatgaaccgg acttttaaag
     3961 tactcagcga cctggtactg atggtctttc tgcggcggta tatgcaaaag ccgatgcact
     4021 tattcggcac tatgggtttt atcagtttcg gactggggct gatcatcaac ctgtatctgc
     4081 tgggactgaa gctgatggga aataatatcg ggggaaagcc gctgctgatt ctcggactga
     4141 tcctcttact gggaggtatt caactgatca cgattggtat tgtggcagat atcaatatcc
     4201 ggacctatta tgagtcccag aataagaaga cctacaacat ccggaaaatt tacaaaggga
     4261 aaaacataat ttcctgaggc acttattgag ccccttttat ttcataagca gctcactgtt
     4321 tgcattaaac cagtcataaa aggcggtcaa tccatctttc agtttggttt gcggatcata
     4381 gcccagtaaa gatttggcct ttgacacatc agcaaaggtc cggggcacat caccgggttg
     4441 ttccggatgc atttctataa ccgctttctt gccgattaca tcctcaatgg cttcgatcag
     4501 ttctttcaat gaaacagtat aattattacc caggttgatt atttcaaaat cactgccggt
     4561 ataatgaata gcgccgataa tgccttttac ggtatccccg acaaatgtat aatcacggct
     4621 ggtactgcca tccccgaaca tctggatcgg ctttccctgc aggataaatt tggtgaattt
     4681 gtgaattgcg agatcaggcc gctgtcccgg accataaacg gtaaaaaaac ggagtgccag
     4741 gaagcggatg ccaaataaat gactgtagac atgccccatc atttccccgg cgagtttggt
     4801 accggcgtaa ggactgatcg gcatcagctg ctcatcttcc ttccagggat aatggtcatt
     4861 caccccgtat acactgctgc tggaagcaaa tacaaattgt tttattttat ttaaccgggc
     4921 aaaatccagc atttgctgta acccgattac gtttgcctgt tggtaagcca gtgggttttc
     4981 aatactcggc ctgacccctg ctttggccgc aatatgcacg attacatcca cgggttcctt
     5041 tattagcccg gtcatttcct ccgcagagca ggtggaaata tctgcctgaa taaactggta
     5101 cccgggaagg gcaataaaat cacggatatt aaaacgttta atttcctctt tgtaaaaggg
     5161 gtcaaaattg tcgatacagg tgatccggac cgacgggtcg tcctggaata atttgcgggt
     5221 aaggttactg ccgataaatc cggctcctcc ggtgatcagg atatgcatgg tggtataatg
     5281 ttggggcaag atagaaaaat aaactggcta ataaaagagg caaacatgca ttcttgcctt
     5341 ccccgtg
//
LOCUS       DAOPMW010000228         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18120_length_5289_cov_5.525602, whole genome shotgun sequence.
ACCESSION   DAOPMW010000228 DAOPMW010000000
VERSION     DAOPMW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18120_length_5289_cov_5.525602"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLU11_10670"
     CDS             <1..469
                     /locus_tag="PLU11_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPN59629.1"
                     /translation="TTRRIDSLYWKEAPAGNIPYKVVVMVRATDPRGYGDYIRYFTKQ
                     NRDPFYPGLNSVYDDQVIDGTTYEIQVERGILRNGIAGEAYSFFDKGDTVTLKLCNID
                     KATFDFWRTMEFTYSSVGNPFSSPTKVMSNIKGGALGYFGGYAAQYKGIVIPQ"
     gene            637..1578
                     /gene="trxB"
                     /locus_tag="PLU11_10675"
     CDS             637..1578
                     /gene="trxB"
                     /locus_tag="PLU11_10675"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPN59630.1"
                     /translation="MDATEKVHCLIIGSGPAGYTAAIYASRAGLNPVLYQGIQPGGQL
                     TITTEVENYPGYPEGIQGPEMMVHFEKQAARMGADIRYGLATAVDFSGPVHKVQIDEE
                     KWIEADAVIISTGASAKWLGLESEQRLNGFGVSACAVCDGFFFKGKEVAIVGAGDTAA
                     EEALYLSKICSQVHMIVRRDEMRASKVMQERVKNTPNIKIYWNSVTDEVLGDKKVHAV
                     RIHNTKTDVKEEVPVSAFFVAIGHQPNSGIFKPFIDMDEAGYIKTIPGTSKTNVEGVF
                     ASGDVQDKIYRQAVTAAGSGCMAALDAERYLSAKGLH"
     gene            1578..1940
                     /gene="folB"
                     /locus_tag="PLU11_10680"
     CDS             1578..1940
                     /gene="folB"
                     /locus_tag="PLU11_10680"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPN59631.1"
                     /translation="MPAVMSIELRDLHFHAFHGLYPEERKTGNDFRVWLQVDYTPPEE
                     VITAIEDTINYATLFEMVQKAMQNPVELLETLVMDIAGSIHVRFPQVIKISISIHKLH
                     PPIARFNGEVGVKYEKEY"
     gene            complement(1937..3298)
                     /locus_tag="PLU11_10685"
     CDS             complement(1937..3298)
                     /locus_tag="PLU11_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPN59632.1"
                     /translation="MSANTHSQLQVGISNRQIFRIALPISLAILIPQLNFIINNIFLG
                     HYNGDAYALAVAGITGVYYLIFAAIGYGLNNGLQALIARRAGENRPEEIGKLFTQGVL
                     IGLLVAIAGIIITWVLTPAIFSLIIHDPAVQEKAVSFLRIRIWGLPFLYIYQLRNALL
                     VGTNQSRYLVAGTLAETLANVGLDYLLIFGHWGLPELGFNGAAIASVIAEFVGMFVIF
                     LVIRWKGISKRFGLFKDMRWNKENSALIFSMSAPLMFQHAISIISWQFFFLLTEHHGD
                     MALKVSNVMRNIFGVFGCVTWAFAATTNAMVSNVIGQNKKDQVVPLIIRIVRLSTGWA
                     LIACTLLNIFPETFLSIFGQGEVFIQAALPSLRVVTAAMILMSFSIILLSAVTGTGNT
                     KITFLIELFAIIMYSSYVYLVNEYFFLPIHIGWMSEWLYWLCLFVPSLWYIRSGRWKA
                     KVF"
     gene            3366..4172
                     /locus_tag="PLU11_10690"
     CDS             3366..4172
                     /locus_tag="PLU11_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPN59633.1"
                     /translation="MKQLFTIENNPETAVQPVLSLRLGNHHLGYSITSPDGDKLYSLA
                     YYRTEQTDTAALEELLAQTPALHNHFYSVQVAWDFTGNALLSSVEHQPEESGTMLRAL
                     FGTNGQEEVITENISNWQLFNVYAVPAGLLQKIKQQYPAAQSRHQISLSIKQVIAATE
                     EGNMYVDFRPDDFTVLLVKSSRLLLAQTYTYSTPEDVVYYLLKICSRLGLSQQELQLQ
                     VSGLIDSDSALYKELYQYFLHIGFRESGWQGNDYPAHFFTTLNDLARCAS"
     gene            4160..4705
                     /locus_tag="PLU11_10695"
     CDS             4160..4705
                     /locus_tag="PLU11_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPN59634.1"
                     /translation="MRIIGGEHGGRKFNPPTNMPYTRPTTDIAKEGLFNVLQHKMDFE
                     NLKTLDLFGGTGSISYELASRGVPDLTIVEKDHAMFDFIKKTAAALKLENFKIIKMDV
                     FKYIQQCTERFDFIFAGPPYALTNIDDLPRLIFEKQLLNKEGRFVLEHTPRNDYKATP
                     FFVAERNYGTTVFSTFVEDRD"
     gene            4708..5277
                     /locus_tag="PLU11_10700"
     CDS             4708..5277
                     /locus_tag="PLU11_10700"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPN59635.1"
                     /translation="MTKKELRNFYKEKRKALTTGEQSRLDDLLLIQFQLADIPFVETL
                     FSYWPMATMREPDTHLFADFMEFRNPELRVAYPRITSDGNTMISVLTDDSTRFLQNGF
                     GTYEPENGEILSPDTIDIIFVPLLCFDKNGYRVGYGKGYYDRYLATCNPDCIRVGFSY
                     FEPIDEIPDKHEFDVPLSLCMTPQSIYVF"
BASE COUNT         1475 a         1276 c         1252 g         1286 t
ORIGIN      
        1 tacaacgcgg cggattgatt ccctgtactg gaaagaagcc ccggcaggca atatccctta
       61 taaagtggtg gtgatggtgc gggccactga tccacgtggc tatggtgact acatccggta
      121 ttttaccaaa cagaaccggg atcctttcta tcccggactg aattccgttt atgacgatca
      181 ggtgattgac ggtaccactt atgaaatcca ggtggaacgg ggtatccttc gtaatggcat
      241 tgccggcgaa gcgtatagct tttttgataa aggggatacc gtaaccttga aactctgtaa
      301 tattgataaa gccacattcg acttctggag aaccatggaa ttcacttatt ccagtgtcgg
      361 aaaccctttt tcttcaccca ccaaagttat gagcaatatc aagggtgggg cacttggcta
      421 cttcggtggc tatgcagcac aatacaaggg aatcgtgata ccccaataat aagtacggag
      481 ttagaagtac gaagtacgac tgacgtgctt tgaataattg ttcttagcat gggctgctcc
      541 tgacacccga cacctgacat cttacacctc tcaacctgtc aaccttttcc ttaattttgc
      601 caactgacca cctttaaccg gtcatttatt tataccatgg acgccacaga aaaagtacac
      661 tgcctgatca ttggttcagg tcctgccgga tatactgccg ctatttatgc cagccgtgct
      721 ggtctcaatc ctgttttata ccagggtata caacccggtg gtcagctgac catcactact
      781 gaagtggaaa attatcccgg ctatccggaa gggatccagg ggcctgaaat gatggtgcat
      841 tttgagaaac aggcggcccg tatgggtgcg gatatacgtt acggtctggc taccgctgta
      901 gacttttccg gacctgtaca taaagtacag atcgatgaag aaaaatggat cgaagctgat
      961 gcggtgatca tttcaaccgg tgcctccgct aaatggctgg ggctggaaag tgagcaacgc
     1021 ctcaatggtt ttggcgttag tgcctgcgcg gtttgtgatg gtttcttttt taaaggaaaa
     1081 gaagtggcaa ttgtaggagc aggtgatacg gccgccgagg aagcactcta tctttccaag
     1141 atctgttcac aggtgcatat gatcgtacgc cgggatgaaa tgcgcgccag taaagtgatg
     1201 caggaaagag tgaagaacac cccgaatata aaaatatact ggaacagtgt aacggatgaa
     1261 gtactcggcg acaaaaaagt acatgccgta cgtatccaca atacaaaaac ggatgtgaaa
     1321 gaagaagtac cggtcagcgc cttcttcgtc gccatcggcc atcagcccaa ttccggtatc
     1381 ttcaaaccct ttatcgatat ggatgaagcg ggctatatca aaaccattcc cggtaccagc
     1441 aagaccaatg tggaaggggt gtttgcctcc ggtgatgtgc aggataaaat ttaccgccag
     1501 gctgtgaccg ctgccggcag tggctgtatg gccgccctgg atgcagaacg ttacctcagc
     1561 gccaaaggtc ttcactgatg ccggctgtaa tgtccatcga gttgcgtgat ctgcatttcc
     1621 atgctttcca tggcctctat ccggaggaac ggaaaacagg aaatgatttc cgcgtatggc
     1681 tacaagtgga ttatacaccg ccggaagaag tgatcacggc tattgaggat acgattaact
     1741 atgccactct ttttgaaatg gtacagaaag cgatgcaaaa cccggttgaa ttactggaaa
     1801 ccttagtgat ggatatcgcc ggcagcattc atgtccgttt tccccaggtc ataaaaatca
     1861 gtatttcgat tcataaactg catccgccta ttgcccggtt taacggggaa gtaggggtga
     1921 aatatgaaaa ggaatattaa aatactttag ccttccaacg gccactgcgg atataccaga
     1981 gggaggggac aaaaagacaa agccagtaaa gccactcact catccagccg atatgaatcg
     2041 gcagaaagaa atactcattc accaggtaaa cataggatga atacataatg atcgcaaaca
     2101 gctcgatcag gaatgtgatc ttcgtattgc cggttccggt aaccgcactc agcagaatga
     2161 ttgaaaaaga catcaggatc atcgccgctg taaccacccg cagggatggc agcgctgcct
     2221 ggataaatac ctctccctgg ccgaaaatgg ataaaaaggt ctccggaaaa atattgagga
     2281 gcgtacaggc aatcagtgcc caacctgtac tcagccgcac aatgcggatg atcaggggca
     2341 ccacctggtc tttcttattc tgaccgatca cattactcac catggcattg gtggtggcgg
     2401 caaaagccca ggttacacaa ccaaaaaccc cgaagatgtt ccgcatcaca ttcgacactt
     2461 tcagggccat atccccatga tgctcggtca ataaaaagaa aaactgccag ctgatgatac
     2521 tgatcgcatg ctggaacatg agcggggcag acatcgaaaa gataagcgcg ctgttttctt
     2581 tattccaccg catgtcttta aataacccaa accgtttact gattcctttc cagcggatca
     2641 ccaggaagat gacaaacata cccacgaatt ctgcaatcac cgatgcgatg gccgccccgt
     2701 taaacccgag ttccggcaac ccccaatgac caaaaatgag caggtaatcc agtccgacat
     2761 tcgccagggt ttccgccagg gtgccggcta ccaggtaacg gctctggttg gtgccgacca
     2821 ggagcgcatt ccgcagctgg tagatataca gaaaggggag cccccagatc cggatacgca
     2881 ggaagctgac ggctttttcc tgaacagcag ggtcatgtat aatcagactg aaaatagcag
     2941 gcgtaagcac ccaggtaata ataattcccg caatagccac cagcaacccg atcagtacgc
     3001 cctgggtaaa gagcttaccg atttcctccg gcctgttttc cccggctctg cgggcgatca
     3061 gggcctgcaa gccattattg agtccatatc cgatcgcggc aaaaatgagg taatagaccc
     3121 cggttatacc ggccacggcc aatgcatagg catccccgtt gtaatgtccc aggaaaatat
     3181 tattgataat aaaattcaac tggggaatca ggatggccag actgatgggc agggcgatcc
     3241 ggaagatctg ccggttgctg atccccacct gtaattggct atgtgtgttg gcggacatgg
     3301 taaaaaggag gcaaacctac gatatttaaa tttttgtcta ttattgcacg aattgaaaac
     3361 cgattttgaa acagttattt accatagaaa ataatccgga aacagccgtt cagcccgttc
     3421 tttccctccg gctgggtaat catcacctgg ggtattccat taccagtccg gatggagata
     3481 agctgtacag cctggcctat taccgcaccg aacagaccga taccgccgca ttggaggaat
     3541 tactggccca aacacccgca ttgcacaacc atttttacag tgttcaggtg gcctgggatt
     3601 ttaccggcaa tgccctgtta tcttcagtag aacatcagcc ggaagaatcc ggtacgatgc
     3661 tcagggcctt attcggtaca aacggacagg aagaagtaat cacggagaat atcagtaact
     3721 ggcaattatt caatgtgtat gccgtaccgg ccggacttct gcaaaaaata aaacaacaat
     3781 acccggctgc acagagccgt caccagatct cactttccat taaacaggtg attgccgcca
     3841 cagaagaagg aaatatgtat gtggatttcc gcccggatga tttcaccgta ttgctggtaa
     3901 aatcaagccg gctgttactg gcacagacct atacatactc cacaccggaa gatgtggtgt
     3961 attacctgct taaaatatgc agccggctgg gtctttccca acaggaattg cagttacagg
     4021 tatccggact gatcgatagt gattctgcct tgtataaaga actttaccag tatttccttc
     4081 atatcggatt ccgtgaatcc ggttggcagg gaaatgacta cccggcccat ttttttacca
     4141 cccttaacga cctggcccga tgcgcatcat aggaggtgaa catggcggac gaaaattcaa
     4201 cccgcccacg aatatgccct atacccgtcc caccacggat atagccaagg aaggattatt
     4261 caatgtactc cagcataaaa tggatttcga aaacctgaaa acactggatt tgtttggcgg
     4321 aacgggcagt atcagttatg aactggcgtc aaggggtgta ccggacctga ccattgtgga
     4381 gaaggaccat gccatgtttg attttattaa aaaaacggca gcggcattaa aactggaaaa
     4441 tttcaaaata atcaaaatgg atgtgtttaa atacatccag caatgtacgg agcgctttga
     4501 ttttattttt gcaggaccgc cttatgcgct caccaatatc gacgacctgc cccggctgat
     4561 ttttgaaaaa cagttactga ataaagaggg ccgcttcgtc cttgagcaca cgccccggaa
     4621 tgactataaa gccaccccgt tttttgtagc ggaacggaac tacggtacca ctgtattctc
     4681 tacttttgta gaggacaggg actaactatg acgaagaaag aactccgtaa tttttacaag
     4741 gaaaaaagaa aggcactgac cactggtgaa cagtccaggc tggatgatct gctgctcata
     4801 cagttccagc tggcggatat cccttttgtg gaaacccttt tttcctactg gccgatggcg
     4861 accatgcggg agcccgatac ccacctgttt gctgatttta tggagttcag aaatccggaa
     4921 ctccgggtag cctatccgcg gatcacttcg gatggaaaca ccatgatttc agtgttgact
     4981 gatgactcca cccgtttttt gcaaaatggg tttggtacat acgagccgga aaacggcgaa
     5041 atcctgtctc cggacacaat cgatatcata tttgtgcccc tgctttgttt tgataaaaac
     5101 ggctaccgtg tcggctatgg aaaaggctac tatgaccgct acctggccac ttgtaatccg
     5161 gattgtatcc gggtgggatt ctcctatttt gaaccaatag acgaaatccc ggataagcac
     5221 gaatttgacg tacctttaag tctttgtatg acgcctcaat cgatttatgt tttctaattt
     5281 ttttctcaa
//
LOCUS       DAOPMW010000229         5288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18122_length_5288_cov_9.060768, whole genome shotgun sequence.
ACCESSION   DAOPMW010000229 DAOPMW010000000
VERSION     DAOPMW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18122_length_5288_cov_9.060768"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLU11_10705"
     CDS             complement(<1..775)
                     /locus_tag="PLU11_10705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPN59636.1"
                     /translation="MKKVVLFLSLISVCNHLFAQPVSEKIRKSFQRFMSDSQLKHASV
                     SLYVIDAKTGQPVFDVNSQMGLAPASTQKIITSVTAFELLGKNYRYKTRFGLITEQGI
                     IQTICIKPSGDPTLGSNRYTHTNPGRVFDRCFEQLTRNGYKGGLISFLIDSSGFETNP
                     IPDGWVWQDIGNYYGAGSGPINWRENQFDISLKSGEVPGSKTEILLFNGSKDRIGMFI
                     NEVKTAAKGTGDNAYIYLPLSNGEYRIRGTIPAGESNFSI"
     gene            854..2611
                     /locus_tag="PLU11_10710"
     CDS             854..2611
                     /locus_tag="PLU11_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPN59637.1"
                     /translation="MSAATINASIITIGDELLIGQTIDTNSAFIAQELNKIGVWVRRR
                     VAVGDVFEDIWKALDEEGAASDIVIITGGLGPTADDITKPLLCQYFGGRLVVNEEVKA
                     HVKNLFENVYRRPGALLERNLKQAEVPDVCTVLHNARGTAPGMMFYKPRVVAPLNPPK
                     EGNSASQRQKMNYEYRTANTDAYTRLKEFVEENRGNQTPAEEKLWAILRGKKIGGYKF
                     RRQHIIGNYIADFICLNENLIIEVDGNIHQLPENQANDESRTKDLNILGYKVIRFTND
                     EVLFNSDHVISSILSALKKENIELPESQSPPLGGGGASTIFISLPGVPHEMKGLITDQ
                     VIPRLMKEFTMPVIIHRTAFTAGQGESMLAEMLMDFEASLPDSVKMAYLPNYGMVKLR
                     LTAKGITREEVETILNPVFDRLLEEVKNFLVTDTDEGLEVVVGKILKAKGLTLGTAES
                     CTGGHIAQLITSVPGSSAYFKGGIVSYANEVKTGVLGVAPETLQSAGAVSEETVRQMV
                     QGAIRVLEVDYAVAVSGIMGPDGGSPEKPVGTVWIAVGNKEKTDAIRLNLRFDRQRNI
                     GLAAVNALNYLRKFILTNS"
     gene            2748..4103
                     /locus_tag="PLU11_10715"
     CDS             2748..4103
                     /locus_tag="PLU11_10715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPN59638.1"
                     /translation="MAIVDLIMPKLGESIMEATVLKWHKQPGDTVKMDETVLEIATDK
                     VDSEVPSTAEGVIEEILFKVNDVVPIGTVIARIRSGAAATSTPAPAPVPEKTPDTPAP
                     VKQTQDIEYVQSVTPNFQPPTSNLQPSSANRFYSPLVLNIAASEGVSMSELEYIPGTG
                     NEGRVTKKDILQYVQEKKAGKAGSPQQAVGSQQQVTVIKESSTPTTNNQQLTTYSGNV
                     EIIEMDRMRKLIADHMVRSKQTSPHVTSFTEADVTNLVQWREKVKKDFEKREGTKITF
                     TPIFIEAIVKCIKKFPLINCSLDGDRIIVKKDINIGMATALPSGNLIVPVIKNADQLN
                     LVGLSKQVNGLADAARNGKLRADDTQGGTFTMTNVGTFGSLMGTPIINQPQVAIMAVG
                     AIKKRPVVVETPAGDSIAIRHMMYLSMSYDHRIVDGSLGATFLTAVAKELENFNPERE
                     Y"
     gene            complement(4242..4751)
                     /locus_tag="PLU11_10720"
     CDS             complement(4242..4751)
                     /locus_tag="PLU11_10720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPN59639.1"
                     /translation="MKYLLLIALFSLGACGNTIYIVRHAEKEPVPAGSSQMMANNPPL
                     SEAGKERSFALRDRLRHERIRFIFSTNTTRTVSTAQPLNELLGTTRIELYSSKKDSTD
                     FFISQLKSIKKGNILVVGHSNTIDDIANKLCGKTVVPGDLDEKEYSNLFVVKRKGGRY
                     VFKRKRYGN"
     gene            4837..>5288
                     /locus_tag="PLU11_10725"
     CDS             4837..>5288
                     /locus_tag="PLU11_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59640.1"
                     /translation="MKSLSETWFAEGRIDFELKKYTLLAYLQEVNKYFDENKLYPQLA
                     DIIFHYNNIVAFRENKKYLQEHFPKKLTGLQMEKLQLIYEQMLEDDELMQELENIIHY
                     SASRIKKTISNGTEIYEFVEDKICITPIGLIPLDTKEGYFFLSAGNVK"
BASE COUNT         1554 a         1214 c         1224 g         1296 t
ORIGIN      
        1 tgatactgaa attggattct cctgcaggaa tggttccccg aatcctgtat tccccattac
       61 tcaatggcaa gtagatataa gcattgtcac ccgtaccctt cgcggctgtt ttcacttcat
      121 tgataaacat cccaattctg tctttgcttc cgttaaataa tagaatttcc gtttttgatc
      181 caggcacttc tccggatttc agcgaaatat caaattgatt ttcccgccag ttgatgggcc
      241 cggatccggc accatagtaa tttccaatat cctgccagac ccatccatcg ggaatgggat
      301 ttgtttcaaa acctgaacta tcaattaaaa aactaatcag tcctccttta taaccgttcc
      361 tggttaattg ttcaaagcac ctgtcaaaaa cacgaccggg gttggtatgc gtataccggt
      421 tgcttcccaa tgtaggatcg ccggaaggtt ttatacaaat tgtctggatg atcccctgtt
      481 cagtaattaa tccaaaccgg gttttatacc ggtagttttt tcccagcaac tcaaacgccg
      541 tcacactcgt aataatcttc tgcgtggaag caggggccaa ccccatttgt gaattgacat
      601 caaagacggg ttgcccggtt tttgcatcga tcacgtacag ggataccgac gcatgtttca
      661 actgactgtc ggacataaag cgctgaaagg atttccggat cttctctgaa acgggttggg
      721 caaacaggtg attacagacg gatatcaggg acaggaacag aacaactttt ttcatcattt
      781 atggttataa cttcgcgcca gcctgccggc aggcaggttc aaattaattc atttcccgct
      841 aatactttct taaatgagtg cagccactat caatgcctcc atcatcacca tcggagatga
      901 actcctgatc ggtcagacca tcgataccaa ttccgctttt atagcccagg aactgaataa
      961 gatcggggtg tgggtacgac gccgcgtcgc cgtgggtgat gtatttgagg atatctggaa
     1021 agccctggat gaagaaggcg ccgcttccga tattgtcatt attaccggcg gattgggacc
     1081 caccgcggat gatatcacca aacccttgct ctgccaatat ttcggaggca gattagtggt
     1141 gaatgaagaa gtcaaagccc atgtgaaaaa cctgtttgaa aatgtatacc gccgtccggg
     1201 tgccctcctc gaacgcaacc tgaaacaagc cgaagttccg gatgtatgta ccgtgttgca
     1261 taatgcgagg ggaacggcgc cggggatgat gttctacaag ccccgtgttg tggcccccct
     1321 aaatcccccc aaagagggga attcagcctc ccaaagacag aaaatgaact acgagtaccg
     1381 gactgcaaat acggatgctt atacccggct aaaggaattt gttgaagaaa acagagggaa
     1441 ccaaacacca gcagaagaaa aattatgggc gattttaaga ggaaaaaaaa taggtgggta
     1501 taaattcaga agacagcata ttatcggaaa ctacatcgca gattttattt gtcttaatga
     1561 gaatctgatc atagaggttg atgggaatat tcatcagctt cctgagaatc aggcaaacga
     1621 tgaaagcaga accaaagatc ttaacatact tgggtataag gtaatccggt ttactaatga
     1681 tgaggtgttg ttcaattcag accatgtaat ctccagtatt ctctccgcat taaaaaagga
     1741 aaatatcgaa ttgccggagt ctcaaagccc ccctttgggg ggtggggggg cctcgaccat
     1801 ctttatctcc ctccccggtg tgccccatga aatgaaagga ctgatcaccg accaggtcat
     1861 cccaaggctg atgaaggaat tcaccatgcc ggtgattatt caccggacag cctttacggc
     1921 cggacaaggc gaatccatgc tggccgaaat gctcatggat tttgaagcaa gtttgccgga
     1981 ttccgttaaa atggcctacc tgcccaatta tggaatggtc aaactccggc taactgcaaa
     2041 aggaattaca cgggaagaag tggaaaccat actcaacccg gtctttgacc ggttgctgga
     2101 agaagtaaag aatttcctgg tcaccgatac cgatgaaggc ctggaagtag tggtaggcaa
     2161 aatcctgaaa gcaaaaggtc ttacactggg gactgctgaa agctgtaccg gcggacatat
     2221 cgcccaactg atcacctccg tacccggatc atctgcctat tttaaaggcg gcatcgtcag
     2281 ttatgccaat gaagtcaaga ccggcgtact gggcgttgct cccgaaaccc ttcaatccgc
     2341 cggagctgta agtgaggaaa ccgtaagaca aatggttcag ggtgcaatcc gtgtactgga
     2401 ggtggattat gcagtagctg tttctggtat catgggtcct gatggtggaa gtcccgaaaa
     2461 accggtgggc acggtctgga ttgccgtagg caacaaagaa aaaaccgatg ctatccggct
     2521 caacctccgc tttgaccgtc agcggaatat tggcctcgct gcggtgaatg ccctgaatta
     2581 cctgcgtaaa ttcatcctga ctaacagttg atagtgtttt agggcaaatc attatctttg
     2641 accgttctta aatcgacccg gatcaggaag tttttgtgcc gaaatgaggg ggaccgggat
     2701 actattatta atattgcgat tgctgttata aaaaactaaa gatatttatg gctattgtcg
     2761 atttaatcat gccaaaactg ggtgaaagta tcatggaagc aaccgtgctg aaatggcata
     2821 aacaacccgg ggatacggta aagatggatg aaaccgtact ggagattgcc accgataaag
     2881 tggacagtga agtacccagt actgccgaag gtgtgattga agagatcctg ttcaaggtaa
     2941 atgatgtggt gccaatcgga acggtcatcg cccgtatccg ctccggcgca gcagccactt
     3001 caacccctgc cccggctccc gtacctgaaa aaacgcccga tactccggct cctgttaagc
     3061 aaacacagga catagagtat gtgcaatcag ttacgcctaa cttccaacct ccaacttcga
     3121 acctccaacc ctcttccgcg aaccgcttct actcccccct cgtcctcaac atcgccgcca
     3181 gtgaaggtgt aagtatgagc gagctggaat atattccggg aacagggaat gaaggaagag
     3241 tgacgaagaa ggatatactg cagtatgtac aagagaaaaa agcaggaaaa gccggcagtc
     3301 cccagcaggc agttggcagt cagcaacagg taacagttat taaagaatct tccaccccaa
     3361 caacgaacaa ccaacagcta acaacttatt cagggaacgt ggagatcatc gaaatggacc
     3421 gcatgcgcaa actgatcgcc gatcatatgg tgcgcagtaa acagaccagt ccgcacgtaa
     3481 ccagttttac cgaagccgat gtgaccaacc tggtacaatg gcgggaaaaa gtgaaaaagg
     3541 attttgagaa aagagaaggc acgaagatta cttttacccc catctttatt gaagcaattg
     3601 taaaatgtat taagaaattc ccgctgatca actgctccct ggacggagac cggatcatcg
     3661 tgaagaaaga tattaatatt ggtatggcca ccgccctgcc cagcggtaac ctcattgtac
     3721 cggtaattaa aaatgcagat cagcttaacc tggtaggttt atctaaacaa gtgaacggac
     3781 tggccgatgc ggcccgcaat ggtaaactcc gggcagatga tacccagggg ggaacattca
     3841 ccatgaccaa tgtgggtaca ttcggcagcc tgatgggcac ccccatcatt aaccaaccac
     3901 aggtagcgat tatggccgtt ggggccatta agaaaagacc tgtagtagtg gaaacgcccg
     3961 ccggagacag cattgccatc cgccatatga tgtatctcag tatgagttac gaccaccgga
     4021 ttgtggacgg cagcctggga gctaccttcc tgacggcagt agcaaaagaa ctggagaact
     4081 ttaatccgga acgggaatat taggagagtt tagagcgtct taatggagaa agagattgaa
     4141 agaaaagttt agaaggttta taaagtctga aagcagaaag aggcataaag aagtgtttag
     4201 atgggcattc ttagaaaggg ctgttgacta ctatctacta actagttacc ataccttttt
     4261 cttttgaaaa cataacgccc tccttttcgt ttcaccacaa acaaattact gtattccttc
     4321 tcatccaggt cacctggcac aaccgttttc ccacagagtt tgttggctat atcatcaatg
     4381 gtattggaat gcccgaccac cagtatattt cctttcttaa tggattttaa ttgtgaaata
     4441 aaaaaatcag tggaatcctt tttgctgctg tacagttcaa tccgggtggt gcccaacaat
     4501 tcgttcagcg gctgagccgt ggataccgtg cgggtggtat tcgtagagaa tataaaacgg
     4561 attcgttcat gccgtaatct atcccgcaat gcaaaggacc tttcttttcc tgcttctgat
     4621 aagggcggat tattggccat catctgagag gaaccggcag gcacgggttc tttttccgca
     4681 tgccggacga tataaatagt attaccacag gcaccaagag aaaacaaagc gatcagcagg
     4741 agatatttca ttgattaata tttaacaggt taaagttaaa ccccatttgc actttttccg
     4801 tctttttagt aaattaggtt accaaaaatc cgcaatatga agtcactatc cgaaacctgg
     4861 ttcgccgaag gccgtatcga ctttgaattg aaaaaataca ccctgcttgc ctacctgcag
     4921 gaagtcaaca agtatttcga cgaaaacaaa ctctatccgc aactggccga tattattttc
     4981 cattataaca atatcgttgc tttccgggaa aacaagaaat acctgcaaga gcattttcct
     5041 aaaaaactga ccggtttgca gatggaaaaa cttcaactga tctatgaaca gatgctggaa
     5101 gatgatgaac tgatgcagga gttggaaaac atcattcact actccgccag ccggatcaaa
     5161 aaaacgatca gcaatggtac ggaaatctat gagtttgtag aggataagat ctgtatcacc
     5221 ccgatcggac tgatacccct ggataccaag gaaggttatt ttttcctgag tgccggaaat
     5281 gtaaaaag
//
LOCUS       DAOPMW010000230         5288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18128_length_5288_cov_4.263138, whole genome shotgun sequence.
ACCESSION   DAOPMW010000230 DAOPMW010000000
VERSION     DAOPMW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18128_length_5288_cov_4.263138"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1507)
                     /gene="galK"
                     /locus_tag="PLU11_10730"
     CDS             complement(323..1507)
                     /gene="galK"
                     /locus_tag="PLU11_10730"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPN59641.1"
                     /translation="MKLNADSMAERFIGLFGNAADPMVVRSPGRVNLIGEHTDYNDGF
                     VLPAAINKAAWVAVTSRNDNQVLLFAEDYHAHYNTTLDSLAPTDEHWPNYILGVADQL
                     RKSGIVISGFNLLLAGDIPIGAGLSSSAAVECATLFALNILFDLHLSKAEMAHIAQHA
                     EHDFAGVKCGVMDMFTSLFGKKDEVINLDCRSLFYEYVPLDLKGYKLLLLNTNLNHRL
                     SSSDYNTRRQQCEQGVAWVKEKVPSVEALRDVTSDMLERYVKERDPLIYQRCLYVVEE
                     NHRLQQACRDLKKGDIRSLGKRMFETHEGLSKQYEVSCPELDFLVDAVKGNADVLGAR
                     MMGGGFGGCTINIVKAEAIKNLVSTLRPGYERSTGLSLGDYIVSVENGTELMRKRETV
                     HA"
     gene            complement(1504..2661)
                     /locus_tag="PLU11_10735"
     CDS             complement(1504..2661)
                     /locus_tag="PLU11_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN59642.1"
                     /translation="MRPIIIHTLLLFSTGLLIFSCSSLRNTVSEKEKAADVLAVIHTV
                     NRYWQSNNPDHGRAFWDNAAYHTGNIEVVKLTGDESYRQYSESWAVKNEWKGATSDNP
                     VEWKYSYGEKPEYVLFGDWQICFQTYLDLYQLKPADYKVARAKEVMGYQVRTGKNDYW
                     WWADGLYMVMPVMVKMYKLTGDPVYLDKLYSWFSYANSIMYDSAAHIYYRDAKYVFPK
                     HATASGKKDFWARGNGWVFAGLAKVLQELPVTDPHRDEYIQTFRQMALTLKQYQQPEG
                     YWTRSIMDNTQSPGYETSGTAFFTYGYFWGINNGYLDSITFLPVAEKGWNYLLTLALQ
                     PEGKIGYVQPIGERAIPGQVVDKDSTANFGVGAFLLAACERYRFLERIKQL"
     gene            complement(2665..3981)
                     /locus_tag="PLU11_10740"
     CDS             complement(2665..3981)
                     /locus_tag="PLU11_10740"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HPN59643.1"
                     /translation="MKLTQSIVAIFFLLLTGSLYGQTGKPVLERVIPAGEGWAGNSVN
                     TVVFRKNSLASLNGIQFISYYDAEGNVVLGKRVITDSSWELKRTRFKGKVTDAHRSIS
                     IALDGEGYLHMAWDHHGNHLRYCRSLQPFSLELSDEIPMTGQHESNVTYPEFYRMPDG
                     GLLFFYRDGSSGNGNLIINTYQPAEKKWTVLQDNLISGEGKRNAYWQACVDNRGAIHI
                     SWVWRESPDVASNHDLCYAVSTDGGRTWEKSTGEKYTIPVTQSSAEIICSIPQRSELI
                     NQTSMYVDGKGKIVIASYWKGQGDLPQYQLVYHTGNQWKQADLAFRKTKFSLSGQGTK
                     KIPVSRPQVIAWQRGRKTFAALIFRDEERGSVVSVVSAQLKKKMKWRISDLPGGSAGD
                     WEPSYDTDLWKKENILHLFVQPVFQADAEGVVKSPPSLVKVLECHF"
     gene            complement(4108..>5288)
                     /locus_tag="PLU11_10745"
     CDS             complement(4108..>5288)
                     /locus_tag="PLU11_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN59644.1"
                     /translation="FFRAYAYNILATCFGGVLIQKEPLTKPKTDFTRAPIDSVNNLII
                     EDLLYAAANLPEPGAFGSRTNVNGKPTARANKYQAMQLLAEVYIRAGKPALAEAQCDL
                     IINSGKFALNTTRFGIKSSQPGDPFNDMFWYGNQRRYQGNKEAIWVMEQENPTNIPGG
                     ITNNPQQRRNWGAAYYQISGMKLCDSLGGRGIARMRLTNWVIYDLYETADMRNSQYNL
                     RRKFYYNTPGNPNYGQQVPYTGADTVFKICPHTTKWYEFNPNDEFGFAMIKDFMLMRL
                     GETYLLKAEAQLNQSNPNGAATTINVLRTRANASQVTGSQITLDFILDERARELLAEE
                     NRRMTLMRTGKLVTRTTAYNTQTINPVLGISSKNLLLPIPLSEINLNKDAVLDQNPGY
                     "
BASE COUNT         1324 a         1332 c         1153 g         1479 t
ORIGIN      
        1 atcatctttg atcaacgatg ctttgtctgc atacaacata aacgacgcat gcggggcttc
       61 cttattgact tcgtataaga gagggttttc ccagtcattt acggggggtt gagccaccag
      121 tccctgaaca atgctgatac tgaaaagaat agccaggtta aattttcgaa tcataacatt
      181 tctttattaa agtaactaag ataatactga ataaaccctg ttgtaaaaac aacagggttg
      241 tttgcttatg cttgctatat gacaaccact tactcttccc gcaggcgggc cggagtaagt
      301 ataatctgaa agaactattt aatcaggcat gaaccgtttc ccttttccgc atcagctcag
      361 ttccattctc caccgaaaca atataatcac ccagtgatag tcctgtggat ctttcatacc
      421 cgggcctaag cgtacttacc aggtttttaa tggcttccgc tttcacaata tttatggtac
      481 atccgccaaa acctcccccc atcatccggg ctcccagtac gtcggcattt ccttttactg
      541 catccaccag gaaatccagt tcagggcaac ttacttcata ttgtttactc agtccttcat
      601 gcgtttcaaa cattcttttt ccgagcgacc ggatgtctcc ttttttgaga tcacggcagg
      661 cctgctgtag ccggtggttt tcctccacca catacaggca gcgctggtaa atcaacggat
      721 ctctttcctt tacatatctt tccagcatgt cactggttac atccctgagt gcctccacag
      781 aaggaacttt ttcttttacc caggctaccc cctgttcaca ttgctgtcgt cttgtattat
      841 agtcggaaga ggagagccgg tgattcagat tggtatttaa aagtaataat ttataccctt
      901 tcaggtccag gggcacatac tcatagaata aactccggca gtccagattg atcacctcgt
      961 cttttttccc gaataatgaa gtgaacatat ccattacccc gcatttaacc cctgcaaaat
     1021 catgttccgc atgctgcgcg atatgtgcca tttccgcttt ggaaagatgc aggtcaaaaa
     1081 gtatattcag ggcaaagagt gtggcacatt caacggcagc agatgaagac aaccctgccc
     1141 cgatcggaat atctcctgcc agcaacaggt taaagccact gatgacaatc cctgatttcc
     1201 ggagttgatc ggctaccccg agtatgtagt tgggccagtg ttcatcagta ggtgccaggc
     1261 tatccagcgt ggtattataa tgcgcatgat aatcttcggc aaaaagtaat acctgattat
     1321 cgttccggct ggtaaccgcc acccaggccg ctttattgat ggcggcgggt aatacaaacc
     1381 cgtcattgta atcggtgtgt tccccgatca gattgacccg gcccggcgaa cggactacca
     1441 ttggatcagc ggcattaccg aataaaccga taaaccgctc agccatgctg tctgcattca
     1501 gtttcatagc tgttttatcc gctccaggaa gcggtatctt tcacaggcgg ccagcagaaa
     1561 agcaccgaca ccgaaattgg ctgtcgaatc cttatccact acctgcccgg gaatagctct
     1621 ctcgccgatg ggctgaacat atccgatctt gccttccggc tggagtgcca gggtgagtag
     1681 ataattccat cctttctctg caacgggaag gaaggttata gagtccaggt atccgttgtt
     1741 gataccccag aaatatccat aagtaaagaa tgcagtccca ctggtttcat aaccgggcga
     1801 ttgggtattg tccattatac tgcgggtcca atacccttcc ggttgctgat actgtttcag
     1861 cgtcagtgcc atctgcctga atgtttgtat atactcatcc cggtgggggt cggttaccgg
     1921 cagctcctgt aatacttttg ccagtccggc aaagacccat ccgttgccac gggcccagaa
     1981 atcttttttc ccggaagcag tggcatgttt tggaaaaaca tatttggcat cccggtaata
     2041 aatatgcgct gcgctgtcat acataatgct gttggcatag ctgaaccagc tgtatagttt
     2101 atccaggtaa accggatccc cggtcagttt atacattttc accatcacgg gcatcaccat
     2161 atacaagcca tcggcccacc accagtaatc atttttcccg gtccttacct gatagcccat
     2221 cacctctttc gccctcgcca ctttataatc cgccggtttc agctgataga gatcaagata
     2281 ggtctgaaag catatctgcc agtcaccaaa aagcacatac tccggctttt ccccataact
     2341 gtatttccat tcaacgggat tgtctgatgt ggcgcctttc cactcattct tcaccgccca
     2401 ggattctgaa tattgccgat acgattcatc accggtcagt ttaactactt cgatattacc
     2461 ggtatgatat gcggcattat cccagaaagc ccgtccgtga tccgggttat tggactgcca
     2521 gtaacggtta acagtatgaa tgaccgctag tacatcggca gctttctcct tttctgaaac
     2581 cgtgttcctg agtgaggagc aggaaaaaat aaggagcccg gtactaaata ataatagtgt
     2641 atgtataata ataggtcgca ttgctcaaaa atgacattcc agcactttca ccagcgaagg
     2701 tggagacttc accacccctt ccgcgtcggc ctggaataca ggttgtacaa ataaatgcag
     2761 tatattttct tttttccaaa gatctgtatc ataggaaggc tcccaatcac cggcggaacc
     2821 tccaggaagg tcagagatac gccatttcat tttcttcttt agttgagcag acacgactga
     2881 tacaacagat cccctttctt catcccggaa aatcagtgcg gcaaatgttt ttcttccccg
     2941 ctgccaggcg atcacttgcg gtcgggatac cggaatcttt ttagtgcctt gtccgctaag
     3001 tgaaaacttt gttttccgaa aggccagatc ggcctgtttc cattgattgc cggtatgata
     3061 aaccagctgg tattgcggca ggtcgccctg ccccttccaa taagaggcta ttactatttt
     3121 tcctttccca tccacataca ttgaagtctg gttgatcagt tcgcttcgtt ggggtatgga
     3181 gcagataatt tctgcagaac tttgcgtaac agggatcgtg tatttttccc cggtcgattt
     3241 ttcccaggtt cgtcccccat ccgttgatac cgcataacag aggtcatgat tactggccac
     3301 atccggactt tcgcgccaga cccacgaaat atggatcgcc cccctgttat ccacacatgc
     3361 ctgccagtat gcattgcgtt tcccttctcc gctgataaga ttatcctgca gtaccgtcca
     3421 ctttttttca gcaggctgat aagtattaat gatcaggttt ccattaccgg aacttccgtc
     3481 gcggtaaaaa aataataacc cgccatcggg catccggtaa aattcgggat aggtaacatt
     3541 ggattcatgc tgaccagtca tgggtatttc atcacttaac tccagggaaa acggttgcag
     3601 gctccggcag taacgaagat ggttgccatg atgatcccag gccatatgca ggtatccttc
     3661 tccatccagg gcgatgctga tactacggtg agcatcggtc accttccctt taaagcgggt
     3721 tcttttcaac tcccagctgc tgtcagtaat cacacgttta cccaatacca catttccctc
     3781 tgcatcataa taactgataa actggatacc atttaaactg gccagagaat ttttccgaaa
     3841 taccactgta ttaactgaat taccagccca gccttcgccg gctggtatga ccctctccag
     3901 cacgggtttc cctgtttgtc cgtacaaaga accggttaac aacaggaaga aaatggctac
     3961 tatggattgg gtcagtttca agggagaaac gtaaataatt attgtaattc gtcaggcatt
     4021 attccttatt gtaaatttat cagattttaa ggaaggccgg ttgtaaaaac aaccggcctt
     4081 tttgctatga cgactgctta tgccacatta ataacccggg ttttgatcca ataccgcatc
     4141 cttattcagg ttgatttcgg agagcggaat cggaagtaac aggtttttgc tgctgatacc
     4201 cagtacagga tttatggttt gtgtgttata agcggtggta cgggttacca gtttgccggt
     4261 acgcatcagg gtcattctcc tgttttcttc ggccagcaat tcgcgggcac gctcatccag
     4321 gataaaatca agggtgattt gtgaaccggt aacctggctg gcattggccc ttgtacggag
     4381 cacattgatg gtcgttgccg ccccattcgg attgctttga tttaactgtg cttccgcttt
     4441 cagcaaataa gtttcgccca aacgcatcag cataaaatcc ttgatcatcg caaaaccaaa
     4501 ctcatcatta ggattgaact cataccattt agtggtatgc ggacagattt taaaaacggt
     4561 atcagcaccg gtgtaaggaa cctgttggcc ataattgggg ttccctggtg tattgtaata
     4621 aaacttccgg cgcaggttgt attgggagtt gcgcatatcg gcagtttcat acagatcata
     4681 aatcacccag ttggtcaggc gcatccgggc aatgcctctt ccacccagtg aatcgcagag
     4741 tttcatacca ctaatttgat aatacgcagc accccagtta cgacgttgtt gcggattgtt
     4801 agtaatacct ccgggaatat tggttggatt ttcctgttcc atcacccaga tagcttcttt
     4861 gttgccctgg taacggcgtt ggttgccata ccagaacata tcattaaacg gatccccggg
     4921 ctgtgaggat ttgattccaa aacgcgtggt gttcagggca aatttgccac tgttgataat
     4981 cagatcacat tgtgcttctg ccagggctgg cttaccggcc cggatataaa cctccgccag
     5041 caactgcatg gcctggtatt tattggcccg ggctgtaggt ttaccattga cgtttgttct
     5101 ggacccaaaa gcaccgggtt ccggcagatt ggccgccgca tacaagaggt cttcaatgat
     5161 caggttgttg acgctgtcaa tcggagcgcg ggtgaaatct gttttaggtt tggtaagggg
     5221 ctccttttga atgagcacgc caccaaaaca agtagccaga atattgtacg cataagcccg
     5281 gaagaagc
//
LOCUS       DAOPMW010000231         5285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18144_length_5285_cov_7.750860, whole genome shotgun sequence.
ACCESSION   DAOPMW010000231 DAOPMW010000000
VERSION     DAOPMW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18144_length_5285_cov_7.750860"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="trpB"
                     /locus_tag="PLU11_10750"
     CDS             complement(<1..892)
                     /gene="trpB"
                     /locus_tag="PLU11_10750"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPN59645.1"
                     /translation="MESMNEKVLIGSPNEQGYYGSFGGAYIPEMLHRNVEELRTKYLE
                     IMYEEEFQKEFQYLLRDYVGRPTPLYLAERLSKRYGAQIYLKREDLCHTGAHKINNTI
                     GQILLAQRLGKTRIIAETGAGQHGVATATVCALKGMECIVYMGEKDIERQAPNVARMK
                     MLGATVIPATSGSKTLKDATNEAIRDWINHPDDTHYIIGSVVGPHPYPDMVARFQSVI
                     SEEMRKQLLEKTGTELPQYVIACVGGGSNAAGAFYHFIEEPSVKLIAVEAAGEGVNSG
                     KSAATTQLGKPGILHGSKSLL"
     gene            complement(970..1596)
                     /locus_tag="PLU11_10755"
     CDS             complement(970..1596)
                     /locus_tag="PLU11_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPN59646.1"
                     /translation="MNIKVCGITEMKQLQQLDGLDIDFAGMIFYKDSPRYIGGKLNAK
                     DIKKADFDLKKTGVFVNPEMIEVLDAIDEYGLEVVQLHGDETPEMCEDLSSEVEVIKA
                     FRIKGDEDIDKLVAPYDAVCDYYLFDTGGLKESFGGTGQQFDWSILAKAKIEKPFFLS
                     GGIGVEDAARVKAFKHPDMFGVDINSKFEVSPGVKDMKLLLQFKQGLK"
     gene            complement(1604..2236)
                     /locus_tag="PLU11_10760"
     CDS             complement(1604..2236)
                     /locus_tag="PLU11_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPN59647.1"
                     /translation="MKLKVCGNILPEQVKAYDDLGVDMAGFNFYPSSPRYMRQKISPE
                     KMKQLKGKFARVGIFVNARYDELVRTVEEYRLDMVQLSGDETPEYCNKIADFISVIKV
                     FRLAGKEHLDPLTIPYKNVCDFFMFDTAGTGYGSTGKKFNWKLLEKSPPSKPYFLSGG
                     IELSDVEKLKNLAAGPAGVKLFAVDVNSQFEMGAGVKDMNKLKQFIDQLK"
     gene            complement(2184..3029)
                     /gene="trpC"
                     /locus_tag="PLU11_10765"
     CDS             complement(2184..3029)
                     /gene="trpC"
                     /locus_tag="PLU11_10765"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPN59648.1"
                     /translation="MDILEKIVAHKKAEIEPFKKLSPVSRFENEGFFWEIQTRSLVHH
                     LLLPNSTGIIAEFKRKSPSKGWFKTKELEVEPVVTAYNKSAAGISVLTDNEFFGGDLD
                     DLIQTKVVTDIPVLRKDFIVDPWQIAEARAFGADVILLIAACLTPAQVKEYAAYAASI
                     GLESILEIHNEEELGHICEEVSMVGINNRNLKTFEVDINTSLQLRKFIPAGKPVIAES
                     GITNVETIVSLKQAGFLGFLIGENFMKADTPAIAFADFIDQLNAKAHEIESMRQHSAG
                     TGESL"
     gene            complement(3408..4403)
                     /gene="trpD"
                     /locus_tag="PLU11_10770"
     CDS             complement(3408..4403)
                     /gene="trpD"
                     /locus_tag="PLU11_10770"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPN59649.1"
                     /translation="MKKILQYLFEHKTLTREAAKEALVNIGRGLYNEHEVTAFMTVYL
                     MRSITIDELQGFRDALLELCVKAELGTYETIDIVGTGGDGKNTFNISTLACFIVAGTG
                     QKVAKHGNYGASSVSGASNVMEQMGYVFSNDSAKLRKELEAANICFLHAPLFHPALKS
                     VGPIRKNLAMRTFFNMLGPMANPANPAFQLVGVYNLEMARIYNYLLQLSGKPFTIIHS
                     LDGYDEISLTSDTKVITQEGERVMTPEQLGKRMVMAEDISGGNTVEEAAAIFRKILKG
                     EGSWAQNAVVLANAAMALNCTGKYQSYDEAYNAAVQSLESEMAWRGLELLIGLQQ"
     gene            complement(4493..5074)
                     /locus_tag="PLU11_10775"
     CDS             complement(4493..5074)
                     /locus_tag="PLU11_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPN59650.1"
                     /translation="MRILVFDNYDSFTYNLVHLVEKITRVKVEVWRNDQISLEKVKDF
                     DKIILSPGPGIPVEAGLLLPLIREYASSKSILGVCLGHQAIGEAFGGTLVNLTKVYHG
                     VATPIEVKSQNSKVKSPLFSGLPERFDVGRYHSWVVSDQDFPEELEITAVDDNGYIMG
                     LQHKTFDVQGVQFHPESVLTPDGEKILRNWLDR"
BASE COUNT         1325 a         1427 c         1012 g         1521 t
ORIGIN      
        1 tcaataagct tttgctgcca tgcagtatac ctggtttacc cagttgtgtg gtagccgcac
       61 tctttccact attcactcct tcaccggcag cttcaaccgc gatcagttta acagaaggct
      121 cttcaataaa atgatagaat gcaccagctg cattactgcc tccacccacg caggcaatca
      181 catactgcgg tagctctgtt cctgtttttt ccagcagctg tttccgcatt tcttcactga
      241 tcacactctg gaaacgcgcc accatatccg gataaggatg cgggcctact acggagccaa
      301 taatataatg ggtgtcatcg ggatgattga tccagtcccg gatggcttca ttggtcgcat
      361 ccttgagggt cttactgcca ctggtggcgg gaattaccgt cgcgcccaac attttcatcc
      421 gggccacatt gggtgcctgc cgttcaatat ctttttcccc catgtacact atgcattcca
      481 tacccttgag ggcacatact gttgctgtag ccactccatg ctgaccggct ccggtctcgg
      541 caatgatacg tgtcttaccc agtcgttgag ccaggagtat ttgcccgatg gtattattga
      601 tcttatgtgc acctgtatga caaaggtctt cgcgctttaa ataaatctgt gcgccataac
      661 gcttactcag tcgctccgcc aggtatagcg gagtcggtct tccgacataa tcacgtaaca
      721 aatactggaa ctctttttga aattcctcct catacatgat ctccaggtac tttgtccgga
      781 gttcctccac attgcgatgc agcatttcag gaatataagc cccgccaaat gaaccataat
      841 acccttgctc attcggcgat ccgatcagca ctttctcatt catactttcc atatcaaacc
      901 gtatttcaac ttttttaatc aacaatactc attcccattt cccactcact actcaccact
      961 caccactccc tacttcagcc cctgcttaaa ctgcaacaat aacttcatat ccttcacccc
     1021 cggtgacact tcaaacttac tgttgatatc caccccgaac atatcaggat gtttaaatgc
     1081 tttgacgcgg gccgcgtctt caaccccgat gcccccactc aggaaaaaag gtttttcaat
     1141 cttggccttg gccaggatac tccagtcgaa ttgctggccg gtgcccccaa agctttcttt
     1201 taatccaccg gtatcgaaga ggtaataatc gcagacagca tcatatggtg ccacgagttt
     1261 atctatatcc tcatcgcctt tgatacgaaa ggctttgatc acttctactt cactgctcag
     1321 gtcctcgcac atttccggtg tttcatcgcc atgtaactgt acaacttcca gcccgtattc
     1381 atcaatcgca tccagcactt cgatcatttc cggatttaca aacaccccgg tttttttcag
     1441 atcgaagtcg gctttcttaa tgtcttttgc attcagctta ccgccgatat aacggggaga
     1501 atccttgtaa aaaatcatac cggcaaaatc aatatccagt ccatccagtt gttgcagctg
     1561 tttcatttcg gtaatgccgc atactttaat attcatactt gttttatttc agttggtcaa
     1621 taaattgctt tagtttattc atatccttta cccctgctcc catttcaaac tggctgttca
     1681 catccactgc gaataattta actcctgccg gaccggctgc caggtttttc agtttttcga
     1741 catccgacaa ctcaattcct ccgctgagga aatacggttt tgaaggaggt gatttttcca
     1801 acaacttcca gttaaacttt ttacccgtgc tcccgtatcc tgtacccgcc gtatcaaaca
     1861 taaaaaaatc gcacacattt ttatacggaa tggtcagcgg atccaggtgt tcttttccgg
     1921 cgaggcggaa cactttgatc accgaaataa agtcggcgat cttattgcag tattccgggg
     1981 tttcatcccc gctcagctgc accatatcca gccggtattc ttctacggtc ctgaccagct
     2041 catcatagcg ggcatttaca aaaataccta cccgggcaaa ctttcccttg agttgtttca
     2101 tcttttccgg gctgatcttc tgccgcatgt aacggggtga ggatggatag aagttaaacc
     2161 cggccatatc cacacccagg tcatcatagg ctttcacctg ttccggcaga atgttgccgc
     2221 atactttcaa tttcatgtgc ttttgcattt aattggtcaa taaaatcagc aaaagcaatc
     2281 gctggcgtat ccgctttcat gaagttttcc ccaatcagaa aacccaggaa accggcttgt
     2341 tttaaagata cgatagtttc cacattagta atcccactct ccgcaatgac cggcttgccg
     2401 gccgggataa atttccgcaa ttgcagggaa gtattgatat ccacttcaaa agtcttgaga
     2461 ttccggttat tgatccccac catactcact tcttcacaga tatgccccag ctcctcctca
     2521 ttatgtattt ccagaatgga ttcaaggccg atactggcgg cataggctgc atattccttt
     2581 acctgggccg gcgtcagaca ggcggcgatc agcaggatca catccgcgcc aaaggcccgg
     2641 gcttcggcga tctgccaggg atcgacgata aaatccttac ggagtaccgg aatatcagtc
     2701 accaccttgg tctggatcag gtcgtccagg tcacccccaa aaaattcgtt atcggtcagc
     2761 acggaaatac cggcagccga tttattatat gcggtcacca cgggttccac ttccagctcc
     2821 tttgttttga accagccctt actcggactc ttgcgtttga actccgctat aataccggtg
     2881 ctattgggca acaacaggtg atgcaccagc gaccgggttt gtatttccca gaaaaagcct
     2941 tcattttcaa accgggacac agggctgagt tttttaaacg gttcaatttc cgcttttttg
     3001 tgggcaacga ttttttctaa gatgtccatg ttaaaacaat tcgttgtaaa acaattaatg
     3061 ttctttctat gatcattgaa aattgaacat tgaattttga gcattttcta aacttaggaa
     3121 aacaatgttc aattttcaac gctcaatttt caatagttga accccttcag ggttccaggg
     3181 cattacctac atccctgacc ccgggtttca cccggggcta ttattgttca accccttcgg
     3241 ggttggtaaa tcaacacaat tttattcaaa tcaacttaat ctccgccaat gaaaattgaa
     3301 caatgagcat tgaatattga gctttgggct tcttagaaaa acaatgttca acctgcctgc
     3361 aggcaggcag gtattcaata agcaatgctc aattctcatt taaagagcta ttgctgtaat
     3421 ccaatcagta gttccagtcc cctccatgcc atttcacttt ccagactctg tacagctgca
     3481 ttgtatgctt catcgtagga ctgatacttt ccggtgcaat tcaaagccat agcagcattg
     3541 gccagcacca ctgcattttg agcccagctg ccttcacctt tcagaatttt ccggaaaatg
     3601 gccgcagctt cctccactgt attcccaccg ctgatatctt cggccattac cattcgttta
     3661 cctaattgtt ccggggtcat gacccgctcc ccttcttggg tgattacctt ggtatcactg
     3721 gtcagggata tttcatcata cccatccaga ctatgaatga tcgtaaatgg cttgccgctt
     3781 aattgaagca ggtaattata aatacgggcc atctccaggt tatacactcc tacgagctga
     3841 aatgccggat ttgccggatt ggccatcggg ccgagcatat tgaaaaaagt ccgcatagcc
     3901 aggttcttcc ggatcggacc aaccgattta agggccggat gaaacaaggg cgcgtgcagg
     3961 aaacaaatat tggcggcttc caattccttc cgcagtttag ctgaatcatt ggagaaaaca
     4021 taccccatct gctccatcac attggaggct ccgctgaccg aagaggcacc atagttacca
     4081 tgcttggcta ctttctgccc cgtacctgcc acgataaaac aagccagcgt ggagatatta
     4141 aacgtgttct tcccgtcacc cccggtaccc acaatatcga tggtctcata ggtgccgagc
     4201 tctgctttta cacaaagttc cagcagtgca tcccggaatc cctgcagttc atcaatggtg
     4261 atactacgca tgaggtaaac ggtcatgaaa gcggtcactt catgctcatt gtataaccct
     4321 ctgcctatat tcaccagggc ctcctttgcc gcttcgcggg tgagggtttt gtgttcgaac
     4381 aagtattgta agattttctt cattagaaaa gtttaaagag tttggtgttt agtcgtgtag
     4441 gtacgcttac ttagaagatc cctgacacct gacagccgac acctgacagc ttttacctat
     4501 caagccaatt cctgagtatc ttctctccgt ccggcgttaa tacactttcc ggatggaatt
     4561 gtacgccctg cacatcaaag gttttatgtt gtaaccccat gatataccca ttatcatcaa
     4621 cggctgtaat ctccagttcc tccggaaaat cctgatcact caccacccag ctatgatacc
     4681 gtccaacatc aaacctttca ggtaagccgc tgaataaagg gctttttact tttgaatttt
     4741 gactttttac ttctatagga gtagcgacac catgatacac cttcgtcaga ttcaccaacg
     4801 ttcccccaaa agcctccccg atcgcctgat gtccgagaca aactcccaat atggatttcg
     4861 aggacgcata ttctctgatc agtggcaaca gcagtccggc ttcaaccgga ataccaggtc
     4921 ccggtgataa aattatttta tcaaagtcct ttactttctc caacgaaatc tggtcattgc
     4981 gccagacctc cactttcacc cgcgtgatct tttcgaccag gtggacgagg ttgtacgtaa
     5041 aagaatcgta attatcaaaa accagtattc tcatcttact tagtttaata gtttagacgt
     5101 ttagtcgttt agacgtttag ttgtttagaa gaacatccgc taaacaacta aacgactcaa
     5161 caactaaaca attttctccg cttccactat cgccttcttc aatgccccca gcttattatt
     5221 cacttcctgt aattcacttt ccgggttact ggccaccacc acaccggcac cggcctggta
     5281 agtga
//
LOCUS       DAOPMW010000232         5244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18335_length_5244_cov_8.499133, whole genome shotgun sequence.
ACCESSION   DAOPMW010000232 DAOPMW010000000
VERSION     DAOPMW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18335_length_5244_cov_8.499133"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="PLU11_10780"
     CDS             complement(<1..2036)
                     /locus_tag="PLU11_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN59651.1"
                     /translation="MKISNRHSGLTILFLLLTLLCFSQKKYAFVSGKVVDDNENPLTK
                     VSVVILGQSNGITTNDSGYFRIRVEADKAFALIFSYAGRRAEQKNFLLSEGEEETVTI
                     RLEKSENTLQEVIVSDQRDRRETGLIRPNPKTILNLPSAITGVESLIKIFVGSNNELT
                     SQYSVRGGSYDENLIYVNDFEVFRPYLVRSGQQEGLSFINPEMVRNISFYNGGYQARY
                     GDKMSSVLDIQYKRPKKFGGSAYVGILEQGLQLEGAGNNNKFSYLLGARNRSNRNLLS
                     RQDTKGNYVPSSADFQALLHYQFSNKWQAELLSNISQTKFTLIPQFSQLTTSVFSPIY
                     SATLGVDIFFEGREKDQYQTNMLGFALINQPTKRLRLKWMASRFANDESENIDITGAY
                     LFGDRDFDKRNPTYGLIVNPLGAGVYQNWARNELNIENWNISHKGNYDLGKHAWQWGL
                     GYDKSKINDKLNEWEFQDSAGYALPYQPNLLQLSKVIKSNASLDINKFSGYIQDNLLL
                     GDSAHSFTVTAGLRFNYNSLNQEFLLSPRLGASWKPASKKDIVYRVALGAYHQPPFYR
                     ELRRYDGTVNSDLKAQKSWQAVGGFDYNFIGLNNRPMRWTTEAYYKSMSDVVPYDVDN
                     VRIRYFGENVAKAYATGIEMRLHGELVPDAESWISIGIMKSAENIEGDHYYRYK"
     gene            complement(2094..2561)
                     /locus_tag="PLU11_10785"
     CDS             complement(2094..2561)
                     /locus_tag="PLU11_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59652.1"
                     /translation="MRLLLTFLIGSLLIVSCGSTPCGPAELNFRLVGFSNSETDTMIV
                     HRLKKNSNEVISRVEYNPSNPVRFEHINDTSILVAYQSDLLMRSNFDYQIEFPVANRS
                     FLITGINEMISYSKSRGLFSNTKEYCMNTISNCRVDGMLQSVLQFPNTIYLRK"
     gene            complement(2584..3234)
                     /gene="rpe"
                     /locus_tag="PLU11_10790"
     CDS             complement(2584..3234)
                     /gene="rpe"
                     /locus_tag="PLU11_10790"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPN59653.1"
                     /translation="MPIIAPSLLAANFLNLSNECEMLNKSEADWFHLDVMDGRFVPNI
                     SFGPMIIEFIRKATTKTCDVHLMIEEPEKYAEEFKKAGADILTVHIEACRHLHRNIQQ
                     IKSLGMKAGVALNPHTPVAGLADILHEIDLVCLMSVNPGFGGQKFIPFTLEKIKQLRK
                     MITDRGLNVHIEIDGGVTQENAQSILDAGADVLVAGSTVFKAPDPIGMIHQLKQLS"
     gene            3228..3425
                     /locus_tag="PLU11_10795"
     CDS             3228..3425
                     /locus_tag="PLU11_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59654.1"
                     /translation="MAYLGDLRGKFTVFETLVEKNQEAKMPKKEPRSQDAKTPRCQKR
                     TKKPRCQEAKEESNLNIQKPS"
     gene            complement(3557..4564)
                     /locus_tag="PLU11_10800"
     CDS             complement(3557..4564)
                     /locus_tag="PLU11_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59655.1"
                     /translation="MNKYAKIGPFIAGILTLISACTDRNDGATTFESLLSGPPYSALT
                     DSIKNDPKKDELYFRRAIQLNSNQQPEAALADFRKAWELKKNEQYALGIGSLLLESKP
                     SEALVFLKEALTVLPESLLLNLTLARSYDNQGKTEEALQLISGLLIRNPEQVDLLKMK
                     ANLLGKKGNIPEAISLLEKAYNLTPYDVELNYILALQYAESKNSRVLDLCDSLARADS
                     LGEHAEPYYYKGIYYTAINDKKKALLAFDEAIKHDYTYFESYIEKGSILFEMKNFTDA
                     LKVLNLALTISPKYPETYYWIAKCQQALGQKEEAKLNYQRAFSLDQSFTSARDSANKL
                     K"
     gene            4706..>5244
                     /locus_tag="PLU11_10805"
     CDS             4706..>5244
                     /locus_tag="PLU11_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59656.1"
                     /translation="MKKIALLLIIAGVIIALPACKNSSKLTAANAAADTLRYPDETRL
                     KNIRQLTFGGDNAEAYWSYDGKYLVFQRTNPKEGINCDQIFVGKVPTKAGEPFTYKLV
                     STGTGRTTCPFFTKDGKHIIYASTHLGSKDCPPTPDRAKYGNRYIWPLYDTYDIFMAD
                     LDGKIVKQLTHSKGYDAEAT"
BASE COUNT         1365 a         1197 c         1231 g         1451 t
ORIGIN      
        1 tttttatacc ggtaataatg atccccttca atattctcgg cactcttcat aataccaata
       61 ctgatccagc tctcggcatc cggcaccagt tcgccatgca gccgcatttc aatgccggtg
      121 gcataggcct tggctacatt ttccccgaaa taacgtatgc gtacattgtc cacatcatag
      181 ggtaccacat cgctcatgct tttataatag gcttccgtgg tccagcgcat gggccgattg
      241 ttcaacccga taaaattata atcaaagcca cccacggctt gccagctttt ctgtgctttc
      301 agatcggagt ttactgtacc atcgtaacgg cgtaactccc ggtagaaggg cggctgatgg
      361 tatgcgccga gggccacgcg gtatacgata tcttttttag aagccggttt ccagctggct
      421 cctaaccggg gagaaagcag gaattcctgg ttgagtgaat tgtaattgaa acgtaacccg
      481 gccgttaccg taaaggaatg ggccgaatca ccgagtaata agttatcctg tatataaccg
      541 gagaatttat tgatatcgag tgacgcattt gatttaataa ccttgcttaa ctgaagcagg
      601 ttgggctggt agggcagggc atagccggca gaatcctgga actcccattc attcagcttg
      661 tcattgatct tacttttatc ataacccagc ccccattgcc aggcatgttt gcccaggtca
      721 tagtttcctt tatgcgagat attccagttt tcaatgttta gttcattccg ggcccagttc
      781 tggtacaccc cggctccaag cggattcacg attagtccat aagtgggatt ccgtttatca
      841 aaatcacggt ccccaaacaa atacgctccg gtaatatcta tattttctga ttcatcattg
      901 gcaaagcggg aagccatcca ttttaagcgg agtcttttag tgggctggtt gatcagggca
      961 aagcccagca tattggtctg gtattggtct ttttcccttc cttcaaaaaa tatatccacg
     1021 cccagggtgg cgctgtatat tggcgagaaa acggaagtgg tgagctgact gaattgcggg
     1081 atcagggtaa attttgtctg tgaaatatta ctcaataact ccgcctgcca tttattactg
     1141 aactgatagt gaagtaaggc ctggaaatcg gccgaagaag gcacataatt ccccttggta
     1201 tcctggcggc tcagcaggtt acggttgctc cggttacgcg cccccagcaa gtaactgaat
     1261 ttattattat taccagcccc ttccagttgc aggccttgtt ccagtatccc cacataggcc
     1321 gatccgccga attttttagg tcgtttatac tgaatatcca gcaccgaact cattttatca
     1381 ccataccggg cctggtaacc gccattgtaa aaactgatat tgcggaccat ttcggggttg
     1441 ataaaactca gtccttcctg ttgtccgctc cgtacgagat acggccggaa cacttcaaaa
     1501 tcgtttacat agatcaggtt ctcatcatag ctgcccccac gtaccgagta ctgggaggtc
     1561 agttcattat tggaacctac aaatattttg atcaggcttt ccacaccggt aatggcggag
     1621 ggcagattga gaatggtttt gggattgggt cgtatcagtc cggtttcgcg ccggtcccgc
     1681 tgatcggaga caatgacttc ctgcaaggtg ttttcgcttt tttccaggcg gatcgtgacc
     1741 gtttcttctt ccccttcact gaggaggaaa tttttttgtt cggcccggcg gccggcataa
     1801 ctgaaaatca gggcaaaggc tttatcggct tccacccgga tccggaaata acccgaatca
     1861 ttggtggtaa taccattgga ctgaccaagt atgactacgg acactttggt taatgggttt
     1921 tcgttatcat ccaccacttt accggataca aaagcatatt ttttctgaga aaaacagagc
     1981 agggtgagaa gcaggaagag tatggtcagc ccggaatggc ggttgcttat tttcataaga
     2041 aaatgatacg acaagatacg aagagttcag tgcaggtgaa agggttctgt cctttatttt
     2101 ctgagataga tcgtgtttgg gaattgcagt acggattgca acattccatc tacccggcaa
     2161 ttggaaatgg tattcatgca atactctttg gtgttgctga ataagccacg ggatttacta
     2221 tacgagatca tctcgttaat tccggtgata aggaaactgc ggttggccac cggaaattca
     2281 atctggtaat caaaattgct gcgcattaag aggtctgact ggtaagcgac caaaatggag
     2341 gtatcattga tatgctcaaa tcttacaggg ttggagggat tatattccac ccggcttata
     2401 acttcattgc tgtttttttt cagccggtga acgatcatgg tgtctgtttc cgaattggaa
     2461 aagccaacca gccggaaatt cagttcggca ggcccgcaag gggtggaacc gcaggagacg
     2521 atcaataaac taccaatcag gaaggtcagg agaaggcgca taaagtgtat gacaaccgga
     2581 atatcaggat agttgcttca gctgatggat cattccgatc gggtcggggg ctttgaaaac
     2641 ggtgctgccg gctaccagca catcggcacc tgcatccagg atcgattggg cattttcctg
     2701 ggtcaccccg ccatcgatct cgatatgtac attcagcccg cggtcggtga tcattttccg
     2761 caactgtttg atcttttcca gggtaaaggg gatgaatttt tgtccgccga agcccgggtt
     2821 cacactcatc aggcagacca ggtcaatctc atgcaggata tcggctaaac cggccacagg
     2881 ggtatgcggg ttcagggcca cgccggcttt catccccaat gattttatct gttgaatatt
     2941 acgatgcaaa tgacggcagg cttcgatatg cacggtcagg atatcggccc cggctttttt
     3001 aaactcttcg gcatatttct ccggttcttc gatcatcagg tgtacatcgc aggttttggt
     3061 ggtggctttg cggataaact caatgatcat cggcccgaaa ctgatatttg gtacaaaccg
     3121 cccgtccatt acatccaggt gaaaccagtc ggcctcgctt ttattcagca tttcgcattc
     3181 attgctgaga tttaggaagt tagcggctaa aagggaaggg gcaatgattg gcatatttag
     3241 gtgatttgcg tggcaaattt acggtttttg aaacacttgt tgaaaagaac caagaagcca
     3301 agatgccaaa aaaagaacca agaagccaag atgccaagac accaagatgc caaaaaagaa
     3361 caaagaagcc aagatgccaa gaagccaaag aagaatcaaa tttaaatata caaaaacctt
     3421 cttagatgta cttgttgtaa atagggaggc ttgctttctt aaatatagct gctacctatt
     3481 gtgaagtaag gttaaattat tttactcttc cccaattggc acattggcat attggcacat
     3541 tggctaattg atctgactac ttcaacttat tagccgaatc ccgtgctgaa gtaaaactct
     3601 ggtctaaact aaaagcccgt tgatagttca gtttcgcctc ttccttctgt ccgagtgcct
     3661 gctggcattt ggcgatccaa taataggttt cggggtattt gggcgagatg gtgagcgcca
     3721 ggtttaagac cttcagggca tcggtgaaat ttttcatttc aaacaagatg gacccttttt
     3781 cgatataact ttcaaaataa gtatagtcat gtttgattgc ttcatcaaaa gccaggaggg
     3841 cttttttctt atcattgatt gctgtgtaat aaatgccttt gtaataatag ggttctgcat
     3901 gttcgcccaa cgaatccgcc ctggcgagtg aatcgcataa atccagcacc cgtgaatttt
     3961 ttgattcagc atattgaagg gccagtatat aattcagttc cacatcataa ggggtgaggt
     4021 tgtaggcttt ctccagcaga ctgatggctt cgggaatatt tccctttttt cccagcaggt
     4081 tggctttcat tttcagaaga tctacctgtt cgggattcct gattaagagg ccggaaatca
     4141 gttggagagc ttcttctgtt ttgccctggt tgtcataact ccgggccagg gtcaggttga
     4201 gcaggaggct ttcaggaagt acggttaatg cttctttgag aaagaccagg gcttcggacg
     4261 gtttggattc caaaaggagg gagccgatgc ccagggcata ttgttcattc tttttcagct
     4321 cccaggcttt gcggaaatcg gccagcgcgg cttcgggctg ttgattcgaa ttaagctgga
     4381 ttgcccggcg gaaatagagt tcatcttttt tagggtcatt tttaatactg tccgtcaggg
     4441 cgctgtaggg cgggccagac aataaacttt caaacgttgt tgcaccatca ttacgatcag
     4501 tacaggcact gatcaaagtc agtattccgg caataaaggg ccctattttt gcatacttgt
     4561 tcatcttcgg tcaatcatta attagaaggc acaaaactac tatttttggc gctgaggtat
     4621 gacaaatagc tgacaaacca acgtaataac aaaaataact ttgcaccctc cggtacagcc
     4681 ccggattcca aagaaacaca tcagtatgaa aaaaattgca ttgctactta tcattgccgg
     4741 ggtaattatt gcccttccgg cctgtaagaa ttcttcgaaa ttaaccgccg ccaatgccgc
     4801 cgctgatacc ctccggtatc ccgacgaaac ccggctgaaa aatatccgcc agctgacttt
     4861 tggcggagat aatgcggaag cttactggag ttatgatggt aaataccttg ttttccagcg
     4921 gaccaatccc aaagagggga tcaattgtga ccagattttt gtaggaaaag tgcccaccaa
     4981 ggccggcgaa ccttttacct ataaactggt cagtaccggt acgggacgaa ctacctgtcc
     5041 tttctttacc aaagacggga aacatatcat ttatgcctcc acccacctgg gtagtaagga
     5101 ttgtccgcct acacctgacc gtgccaaata cggtaaccgc tacatctggc ccttgtacga
     5161 tacgtacgat attttcatgg ccgatctgga tggtaaaatc gtcaagcaac tgacccattc
     5221 caaagggtat gatgcggaag ctac
//
LOCUS       DAOPMW010000233         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18540_length_5197_cov_6.068650, whole genome shotgun sequence.
ACCESSION   DAOPMW010000233 DAOPMW010000000
VERSION     DAOPMW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18540_length_5197_cov_6.068650"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="PLU11_10810"
     CDS             complement(<1..2251)
                     /locus_tag="PLU11_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN59657.1"
                     /translation="MRPLLLSLLLLIQLISISQDGYKTPPKDVADMLMAKRPPNVSVD
                     DKGEWMLFMESSSYPSVAELARPELRIAGLRINPANFAPSRQSFITGLYLKNSKTDKE
                     YKISGLPSPLFAGSVSWSGNDKKIAFTHTTDNRVDLYIIDVATQKATCINKTALNSIS
                     GTYSWYDENTVLYRGTIKPFSAAPPKPAVPDGPTIQENYGKAAPRPTYQDLIKSPYDE
                     QLYAFYSTTQLIKNTNGIETRIGQPAIYSSIVISPDKKYMLIRTLRKPFSYAVPAGGF
                     PALVTITDMNGKLVKQLAELPSAETAPSGYDNVQPFARGFEWRDDEPSTIVWCMPLDS
                     GLIKKQVEYHDAVYAQKAPFTGEAELLFKTKMRFSGIVWGTESLAIVTEGLRSKQLTQ
                     VDRYHPVTGDLEKLITRNTTDAYSSPGFPVTHPNQYGKNVLWTIDNGNKILLNNSTGS
                     SPKGDLPFLMSYDVHKKKADTIWRCAEGYFENVVKVLDAEKGILLTSRESEKEVPNYW
                     IRNTKLKMADRQLTRFGNPYPQMDGVTKEKIKYKRADGVDLTGDLYLPKGYNKERDGK
                     LPVIIWAYPAEYNSAADAAQIRGSEHRYTMVGGGSPVFYVTQGYAVLNNAEMPIVAAS
                     KDKKPNDDFIQQLTMNAEAAINTLDSMGVGDRNKMAVGGHSYGAFMTANLLAHTKLFK
                     AGIARSGAYNRSLTPFGFQNEDRTYWQATELYNKMSPFNYADQIKTPLLLIHGEMDNN
                     TGTFPIQSER"
     gene            2644..>5197
                     /locus_tag="PLU11_10815"
     CDS             2644..>5197
                     /locus_tag="PLU11_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HPN59658.1"
                     /translation="MQLAVVFGYSQQNIKAADRFTRDKGGHDLYGISNHFIENKGQYG
                     HTVKGVEHMGPVLYGYEGLGMPVLFTGKGLIHLQRKLDKISHAEEERLEKMGVPEEEI
                     EKKKIITDRVITMEWLNTNPSLQIQAFDKAAAYHTYRMLNEKVYAFGGITYKNIYPGI
                     DLVYHFTDNAKSGFEYSLKVQPGADLSQVALKYGGDIVSIKTDNKGQLEIRSSIEGVT
                     VSVPITYYGTDLSRAAKAEVNTAFQLDDKTVRFAFPQQYDQSKALIIDPFVSSTINLT
                     GLNTGKAKDVDFDYNGNVYVTGGGAYTGNHSLAKYNAAGALQWTFNGSLTVPSWTYGT
                     YYGGWMVEKPSGNVYLGQGFSPGTGFRVIRISTTGLYDNYITTANASFMEAWKMFWSC
                     NNGSPQILIAGGGLNSNINFGAFTPPSTTVTGLNITGIPYVGGTGWAQDIADFVFDPS
                     TNEMYSIFGSTFGTPGVNNKIYKNTAPYSAASVAWNVPSGFTTIQEAANRPYLVATGL
                     SDNSANMLWVNASYLYYWDGRNLKAFNKATGAGVGTPVLTGNIPFMQGGVIADACNNV
                     FVGEANGTVKVYQFNGTSFSDAPADITIPGMSGKSVYDLAYDESRKLLYVSGDGFVAS
                     MDVSAYCPNTLYTITATPNCATAGVTATIAPVPPVGSTVTYVLFNGTTQVATNNTGVF
                     TGLVSNTTYTIVATVNQFCNGSQASTNFLLPGPLVGATVTNSTCGNTDGMIVATGSGA
                     LAPYTFSIDGTNFQGSGTFAGLGAGVYTITVRDANGCKSTSVVTITNINGPQLAHTNT
                     NAACGINNGSVTATATGGTAPYTYSMNGVTYQNSNFFTGLTGGTYTLYVKDAN"
BASE COUNT         1317 a         1184 c         1271 g         1425 t
ORIGIN      
        1 tgcgttcact ctggatgggg aaagtacctg tattattatc catctcgcca tggatcagca
       61 ggagcggggt cttgatctga tcggcatagt tgaagggact cattttatta tacaactccg
      121 tggcctgcca ataggtacgg tcttcattct gaaaaccaaa gggtgtgaga gaacggttat
      181 aagcgccact gcgggcaatg cctgctttga agagtttagt atgtgccaac aggttggccg
      241 tcatgaaagc gccataacta tgaccgccta cagccatttt attgcggtcg cccacgccca
      301 tcgagtcaag ggtgttgatt gcagcttcgg cattcatggt cagctgctga ataaaatcat
      361 cattgggttt tttatccttg ctggcggcga ctatgggcat ctccgcatta ttcagtacgg
      421 catagccctg ggtgacatag aaaaccgggg agccaccacc caccatcgta taacggtgct
      481 cactgcccct gatctgcgcg gcatcggcgg cggaattgta ttcggccggg taagcccaga
      541 taattactgg cagtttacca tctctttcct tattatatcc ttttggcagg taaagatcac
      601 ccgtaaggtc aacgccgtcg gcccgcttgt atttgatttt ttctttggtc actccatcca
      661 tttgcggata gggatttcca aaacgcgtta actggcggtc ggccattttc aactttgtat
      721 tccggatcca gtagttgggt acttcttttt cactttccct cgaagtaagc agaatacctt
      781 tctccgcatc cagcactttc accacatttt caaaataccc ttcagcacaa cgccagatcg
      841 tatcggcttt ctttttgtgc acatcataac tcatcaggaa gggcagatct cctttgggtg
      901 aactgccggt tgaattattc agcagtattt tatttccatt gtcaatggtc cataatacat
      961 ttttgccgta ctggttagga tgggtgaccg gaaaaccggg acttgaatag gcgtctgtgg
     1021 tattgcgtgt aatcagtttt tccaggtcgc cggttacagg atgataacga tccacctggg
     1081 tcagctgctt gctgcggagg ccttcggtta caatggccag gctctctgta ccccatacta
     1141 ttccgctgaa cctcattttg gttttaaaca acagttccgc ctcaccggta aaaggcgctt
     1201 tctgcgcata tacggcatcg tgatattcta cctgtttttt gatcaggccg ctgtcgagcg
     1261 gcatacacca tacgatggta gacggttcat catcccgcca ttcaaatcca cgggcaaagg
     1321 gttgtacatt gtcatacccg gaaggggcgg tttcggcaga aggaagttcc gccaattgct
     1381 tcaccagttt gccattcata tccgtaatgg tcaccagggc cggaaatcct ccggcaggta
     1441 ctgcatagga aaacggtttc cggagcgttc ttatcaacat gtattttttg tccggtgata
     1501 taacaatact gctatagatc gccggctggc cgatcctggt ttcaatgccg ttggtatttt
     1561 taattaactg cgtggtggaa tagaaagcat acaactgctc atcgtatgga cttttgatca
     1621 ggtcctgata ggtcggacgg ggagcggctt ttccataatt ctcctggatg gtaggaccat
     1681 caggtacggc gggtttgggt ggtgccgcac tgaagggttt aatggtgccc cggtagagaa
     1741 cggtgttttc atcgtaccag gaataagtgc cactgatgga gttcaaggct gttttattaa
     1801 tacaagtggc tttctgtgtg gccacatcga ttatatatag gtccacgcgg ttatcggtgg
     1861 tatgggtaaa ggcgatcttt ttatcattac cgctccagga aacactgccg gcaaaaagcg
     1921 gggaggggag tccgctgatc ttatactctt tatcggtctt gctattctta agataaagac
     1981 cagtgataaa gctctggcgg ctgggtgcga aattggccgg attgatccgc agtcccgcta
     2041 tacgcaactc gggccgggcc agttcggcca ccgagggata actgctgctt tccataaaca
     2101 acatccattc tcctttatca tccacgctca cattcggtgg gcgtttggcc atcagcatat
     2161 cggccacatc tttgggcggg gttttgtagc cgtcctggga aatgctgatc aattgtatta
     2221 gtaacagaag ggataacagg agaggtctca taaaagaagt atttggtgaa agttagtaca
     2281 aatcagagaa agtcaaaagg caaaaggaaa aagtaaaaaa tcctaacact gaagtcccat
     2341 tacatattat aagtaactcc aatagcagat gtattcttct acattttgat gctgagtgac
     2401 acttttgaaa cgaccacttt ttacttttta ctttttcctt ttgacttccc ccaccggtca
     2461 ttttctgcag caaaaaataa attttaaatc taaggttaca tttcccgttt tcgcgcccac
     2521 tcaatgttgt aacatggttt atcggttccc ggttggccag gttttttgac catgactgtt
     2581 tatttgaaca tatcaaaccg ggcgctgggc cagtctgtaa gacgaggtat cttattgctt
     2641 atcatgcagt tagctgttgt attcggctat tcccagcaaa atatcaaagc cgctgaccgg
     2701 tttacccggg ataaaggtgg ccatgatctg tatggtattt ccaaccattt tattgaaaac
     2761 aaaggtcaat atgggcatac tgtaaaaggg gtggagcata tgggaccggt gctatatggg
     2821 tatgaaggat tgggcatgcc ggtattgttt acgggcaaag ggctgattca cctgcaacgg
     2881 aagctggaca aaatttcgca tgccgaagaa gagcggttgg agaaaatggg tgtacccgaa
     2941 gaggaaattg aaaaaaagaa gatcatcacc gaccgggtga ttaccatgga atggctgaat
     3001 acgaatcctt ccctccagat tcaggccttt gataaagcgg cggcctatca tacctaccgg
     3061 atgctgaacg aaaaagttta tgcgtttggg ggaatcacct acaagaatat ttatcccggg
     3121 attgatcttg tatatcattt tacggataac gcaaagagtg gatttgaata tagtctcaaa
     3181 gtgcaacctg gtgccgatct cagtcaggtg gccttaaaat atggcggtga tatcgtttca
     3241 attaaaacgg ataataaagg acaacttgaa atccggtcat ccatcgaagg ggtgacggtc
     3301 tctgttccaa tcacctatta cgggactgat ttaagtcggg ctgcaaaagc agaagtaaat
     3361 actgcttttc aactggatga taaaacagtc cgctttgcat ttccgcaaca gtatgatcaa
     3421 tcaaaagcct taatcattga tccttttgtc agcagcacca tcaatttaac cgggcttaac
     3481 acgggcaagg ccaaggatgt ggattttgat tataacggta atgtatatgt gacgggcggg
     3541 ggagcgtata ccggcaatca cagtctggcc aaatacaatg cagccggcgc tttgcaatgg
     3601 accttcaatg gttctctcac cgttcccagc tggacctacg gtacttatta tggtggctgg
     3661 atggtggaga agccatccgg taatgtttac ctcggccagg gattcagtcc cggcaccggc
     3721 ttccgcgtga tccggatcag caccaccggg ttgtatgata actatatcac cacggcgaat
     3781 gccagtttta tggaagcctg gaaaatgttc tggagttgta ataatggttc cccgcagata
     3841 ctgattgcag ggggtggact aaattccaat atcaacttcg gggcgtttac tcctccgtcc
     3901 actacggtta ccggtcttaa tatcacaggt atcccatatg tagggggaac cggttgggca
     3961 caggatattg cggactttgt ttttgatccg tccaccaacg aaatgtacag catcttcggc
     4021 agtacattcg gtacccctgg ggtcaataac aaaatttata aaaacacagc accttacagt
     4081 gcagcttcag tggcctggaa tgttccttcc ggatttacca ccatacaaga ggcggccaac
     4141 cgtccttacc tggttgccac cggactaagt gataactccg ccaatatgct ttgggtaaat
     4201 gcctcctatt tatattactg ggatggccgt aacctgaaag cctttaataa agcaaccggt
     4261 gctggtgtgg gaacgccggt actcaccggc aatatcccct ttatgcaagg tggtgttatc
     4321 gcagatgcct gtaacaatgt atttgtgggt gaggccaatg gtacggtgaa agtgtatcag
     4381 tttaacggaa cctctttcag tgatgcaccg gccgatatta cgattccggg aatgtcgggc
     4441 aaatcggtat atgacctggc gtatgatgaa tccaggaaat tgttatatgt aagcggggat
     4501 ggatttgtcg cctccatgga tgtatccgcc tattgtccga atacacttta caccataaca
     4561 gctacaccca attgcgccac ggcaggagtt acggctacca ttgcaccggt gcctccggtt
     4621 ggttcaacgg taacgtatgt gttatttaac ggaactacac aggtggcaac caataacacg
     4681 ggcgtattca ccggactcgt ctccaatacc acgtacacca ttgtagccac agtcaaccag
     4741 ttttgtaatg gatcacaggc cagtactaat tttttattgc ccggaccctt ggtcggtgct
     4801 acagtcacca acagtacctg tggaaatacc gacgggatga ttgtggccac cggttccgga
     4861 gctttggcac cttatacatt cagtatcgat ggcactaatt tccagggaag cggcacgttt
     4921 gccggattgg gtgcaggcgt gtatacgata acggtccgcg atgccaatgg ctgtaagagt
     4981 acgagtgtgg ttacgattac taacataaat ggtccgcagt tggctcatac caatacgaat
     5041 gcagcatgtg gtatcaataa tggttcggtt actgctacgg ctactggtgg tacagctccc
     5101 tatacttaca gtatgaatgg agtaacctac cagaacagta atttcttcac cgggcttaca
     5161 ggaggaacgt atacacttta tgttaaagac gcgaata
//
LOCUS       DAOPMW010000234         5196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18546_length_5196_cov_4.803929, whole genome shotgun sequence.
ACCESSION   DAOPMW010000234 DAOPMW010000000
VERSION     DAOPMW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18546_length_5196_cov_4.803929"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLU11_10820"
     CDS             <1..247
                     /locus_tag="PLU11_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPN59659.1"
                     /translation="VDDVIADKIRDTARQVYKVFNCRGVVRIDFIFNEKEGRPYMLEV
                     NTIPGQSEASIVPQQVKVLGWSLKEFYTKLVLECFGE"
     gene            275..1075
                     /locus_tag="PLU11_10825"
     CDS             275..1075
                     /locus_tag="PLU11_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPN59660.1"
                     /translation="MLKAITGRPLWLNVLVGIILAIGIFALFLLSLNWITGHGKSATV
                     PSVTGKTFEQAKTILKKAGFAVEIQDSIYVDTVPALHVIKQIPDADEVVKSNRTVFLV
                     IRRAVPPEVEMPNLTGYSFRNAEMVLKSMDLRVGDTTFKPDFARNAVLEQIYNGVIIK
                     PGTKIRKGSVISLVLGDGIGNQEFSVPIITGMTFCDAKAELESMGLSVGAIVLNGVTD
                     TCNAFIFKQTPGRYDDEKRILRIRSGQTIDVWLQSDRPVTDTVQQLPE"
     gene            1095..1406
                     /locus_tag="PLU11_10830"
     CDS             1095..1406
                     /locus_tag="PLU11_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59661.1"
                     /translation="MENMTVEELKARLDAGEKLNLIDCREPAEYAEFNIGAKLIPLGM
                     IQTMQADELNDLKEEEVIIHCRSGKRSMMACMFLDTMGFKKTVNVTGGVLAWQEKFGS
                     K"
     gene            complement(1862..4021)
                     /locus_tag="PLU11_10835"
     CDS             complement(1862..4021)
                     /locus_tag="PLU11_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN59662.1"
                     /translation="MKRIFMSVCLIGAVISSFAQLKESVKDSFYLLSPVEVKAIRAGE
                     NAPFTKSNLSRKAIAASNLGQDIPYLLNQTPSVVVNADAGNGIGYTGIRIRGTDATRL
                     NMTINGIPYNDAESQGLFFVNLPDLSSSAGSIQIQRGVGTSSNGAGAFGASMNFSTNE
                     VVKESAIELNNSAGSFNSWKNTLRYSSGLVNGFTTDVRLSRISSDGFIDRARANLQSF
                     FISTAWMNNKTSVRFNAFTGKEKTYQAWYGISEADLLAGNRTINYAGTEKPGSPYANE
                     TDNYKQSHYQLFFNQVLNDRLSLNIASFLTRGKGYYEQYKADEDYVKYNLPYPVNGND
                     TAFTSDFIRQLWLDNYFYGSVFSLQYKSNKTQVTGGGGLTRYDGKHYGKVNWASNGLT
                     GLDTWYNLDAFKTDFNLYGKQQTEIAAGVYLFYDLQYRYVKYDINGFRDNPELVINND
                     WHFFNPKAGITLKKNNWKTYLSYGMANKEPNRDDFEAGAALQPRPEQLHDIEAGVERN
                     GKKYNWGATLYYMRYKDQLVLTGRINDVGAYTRTNIPESYRAGIELQGSYNWQSWLNV
                     SGNLSFSRNKVLDMEEYIDDYDNGGQKLNRYASADIAYSPNITGSATIELVPVKRTAI
                     SLISKYVSRQYLDNTSNKARSLDPFFVQDLRLSYTIKSKKLKELNIIGQLNNVFNNEY
                     EPNGYTFSYIYGGQQTTENFYFPMAGINFMIGVNVRL"
     regulatory      complement(4071..4173)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4289..5011
                     /locus_tag="PLU11_10840"
     CDS             4289..5011
                     /locus_tag="PLU11_10840"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPN59663.1"
                     /translation="MKKLCLSLLFLTGFASYLGAQQTINDPNAVKVDVASFHGIEVAT
                     GIELFLNAGNVEEVAISASNKEYRDRIVARVENGILKIYYESKLKSINKKKESKQLKA
                     YVSYKQLDELQANTGALVRFQGVLKTVALDMKVNTGAQVMGEVDIKSLVVKQNTGSQV
                     SLTGKTEQLKVDGDTGSKFKGEEMTTQSCDVTVSTGANVTIDAEKEIMVKASTGGIVK
                     YKGNAAIREIKTSTGGTVKKIG"
BASE COUNT         1471 a         1118 c         1211 g         1396 t
ORIGIN      
        1 tgtggatgat gtgatagcgg ataaaatcag ggatacagcc agacaggtgt acaaggtatt
       61 taattgtcgc ggggtagtgc ggatagattt tatatttaat gaaaaggaag ggcgacccta
      121 tatgctggaa gtaaacacga ttccggggca aagtgaagcc agtatcgtac cacagcaggt
      181 aaaagtattg ggctggagcc tgaaagaatt ttatacaaaa ctggtgctcg aatgttttgg
      241 tgaataaaac cctgtccatt ttaaaaacca tctgatgtta aaagctatta caggtcgtcc
      301 tttgtggctc aatgtactgg tcggtatcat actggccatt ggcatttttg cccttttcct
      361 gctttccctg aactggatta caggtcatgg gaaatcagca accgtgcctt ctgtaaccgg
      421 taaaaccttt gaacaggcaa agacgatcct gaaaaaagcc ggatttgccg tggagataca
      481 ggattccatt tatgtggata ccgtgcccgc cctgcatgtc ataaaacaaa taccggatgc
      541 agatgaagtc gtgaaatcca accgtaccgt attcctggtg atccgtcgcg ccgttccacc
      601 tgaagtggaa atgcccaact tgacaggcta tagtttccgg aatgcagaaa tggtactgaa
      661 aagtatggac ctgcgggtag gcgataccac attcaaaccg gattttgccc gcaatgcggt
      721 actcgaacaa atctataacg gggtaatcat caagccggga accaaaatcc gtaaaggatc
      781 cgttatctca cttgtcttgg gagatgggat cggaaaccag gaattttctg tccccattat
      841 taccggcatg actttttgtg atgccaaagc agaattggaa tccatgggac tgtctgtagg
      901 agcaatcgta ctgaatgggg ttacagacac ctgcaatgcc ttcatattta aacagacacc
      961 cggccgttat gacgacgaga aaaggatatt gagaatccgg tcagggcaga ccatcgatgt
     1021 ctggttgcag agtgatcgtc cggtgacgga taccgttcaa cagctccctg aataaaacaa
     1081 aaaccaaaac gtatatggaa aacatgaccg tagaagagtt gaaagccagg ttggatgccg
     1141 gcgaaaaact gaacctgatc gattgccggg aaccggcgga atatgccgag tttaatatcg
     1201 gggccaaact gattccactt ggcatgatcc aaaccatgca ggcagacgaa ctaaatgacc
     1261 tcaaagaaga ggaagtaatc attcattgcc gcagcggaaa acgaagtatg atggcctgta
     1321 tgtttctcga cacgatgggg tttaaaaaaa cggtgaatgt aacgggagga gtactggcct
     1381 ggcaggagaa gttcggaagt aaataattag ccaattagcc aatgggtttc ttagcattta
     1441 tctttttaaa tagcacattt ttctgtatta gatttttcag ttattaatta gccaattagc
     1501 caatatgcca atgtgccaat tatttacaga tcgaagagta taatcatctt gaatattccg
     1561 agaatcagtc aacgaattat ttataaaaaa aagcccatcg ggcttttttt tataaatacg
     1621 ccttcaataa cataaagaag tttcaatctt tcaggctttt cattcattgg cacattggca
     1681 cattggcaca ttggctaatt ggcacatttt taccggttaa tctaagtcag aaaagagtac
     1741 taaccagtaa ccgacgtact aagaatttca ttggcatatt agctaaatgg cacattataa
     1801 ccggctaaac taaatcagaa aaactacaaa cctgttacca acgtactaag aacagaattg
     1861 actaaagccg cacattcacc ccgatcataa aattgattcc cgccatcggg aaataaaaat
     1921 tctccgtagt ctgttgtccg ccgtaaatat aactgaaggt atagccattg ggttcgtatt
     1981 cgttattgaa tacattattc agctgtccaa tgatattcag ttcctttaat tttttcgatt
     2041 ttatggtata gctcagccgg aggtcctgta caaaaaacgg atccagggac cgggctttat
     2101 tgctggtatt gtcgaggtac tggcggctta catatttgct gataagactg atggctgtac
     2161 gtttcactgg aaccagttca atagttgcgc tcccggtaat gttcggtgaa taggcaatat
     2221 ccgcggaagc ataacggttg agtttttgcc cgccattatc atagtcatca atatattctt
     2281 ccatatccag cactttattc cggctgaagg atagatttcc ggaaacgttc agccagcttt
     2341 gccaattgta ggatccctgc agttcgatac ccgcccggta gctttccggt atattggttc
     2401 ttgtataggc ccctacatca ttaatccggc cggtgagcac cagctgatct ttgtaccgca
     2461 tataatacag ggtggcaccc cagttgtatt ttttcccatt ccgttccaca ccggcttcaa
     2521 tatcgtgtaa ctgttcagga cggggttgta aagcggcgcc cgcttcaaaa tcatcccggt
     2581 tgggctcctt attggccatg ccgtaggaga ggtaagtctt ccagttattc ttcttcaacg
     2641 taataccggc tttggggttg aagaaatgcc agtcattgtt tatcaccagc tccggattat
     2701 cccggaaacc gttgatatcg tatttgacat agcggtattg aaggtcataa aacaggtaca
     2761 cgccggcagc gatttctgtc tgctgtttgc catacagatt aaaatctgtt ttgaatgcat
     2821 ccaggttata ccaggtatcc agcccggtga gcccattgga cgcccagttc actttcccgt
     2881 aatgtttccc atcatatcgg gtcagtccgc caccaccggt aacttgtgtt ttgttggact
     2941 tatactgcag ggaaaagacc gaaccataaa aataattatc gagccacaac tgccggataa
     3001 agtctgaggt gaatgccgtg tcattcccgt tgacagggta gggcaggtta tattttacat
     3061 agtcctcatc cgctttatat tgttcataat aaccttttcc cctcgtaagg aagctggcta
     3121 tattcaggct gaggcggtca ttgagcacct ggttgaagaa aagctggtaa tgcgattgct
     3181 tgtaattatc cgtttcgttt gcatacgggc tgcccggttt ttcggtgccg gcatagttga
     3241 tcgttctgtt tccggcaagc aaatccgctt ctgaaatgcc ataccaggcc tgatacgttt
     3301 tctctttccc ggtaaaagca ttgaaacgta ccgaagtttt gttattcatc caggcggtgg
     3361 agataaaaaa ggattgcagg tttgcacggg cccggtcgat aaatccatca ctgctgatcc
     3421 gggataaacg gacatcggtc gtaaatccgt tcaccaatcc gctgctgtac ctgagggtgt
     3481 ttttccagga gttaaaagaa ccggcactgt tatttaactc gatcgcggac tcctttacca
     3541 cttcattggt gctgaaattc atactggcgc cgaaagcccc ggctccattg gaagaggtgc
     3601 ctactccccg ttgaatctga atactgcctg cagaggaact gagatcgggc aggttgacaa
     3661 agaacaagcc ctgactttcg gcatcattat aggggatacc atttatggtc atattgagcc
     3721 gcgtggcgtc tgtgcccctt atccggatac cggtgtagcc gattccgttt ccggcatcgg
     3781 cattaactac cacagatggg gtttggttga gcaggtaagg aatatcctgc cccaggttgg
     3841 aagcggcaat cgccttcctg ctcagattag atttagtgaa tggtgcattt tcaccggccc
     3901 ggatggcttt cacctccacc ggcgataaca ggtagaagct gtcttttacg ctctctttta
     3961 gttgcgcaaa actgctgatg actgccccaa taaggcagac ggacataaaa atcctcttca
     4021 tttttttaca tttaaccgtt aaaaaataag agggaaacgc tgtggagaaa ggacaactga
     4081 agactccttc ccttcgcagg cattaccctg accaggttct acgggtttga tctcagattc
     4141 cggcttatgc cggaaacacc ccgggaaaca ctgaaaatca ggcctttttg ttacaaaggc
     4201 cctaacaaat acgaaattac aaagaaaaat gatacccggg ttgtaacttt tctacgctgc
     4261 ctttcgtatt acttctaaac catcgtttat gaagaagtta tgtttatcct tgttgttcct
     4321 tactggcttt gcctcctacc tgggagccca gcaaaccatc aacgacccta atgcggttaa
     4381 ggttgatgtg gcctctttcc atggaattga agtagctacc ggaattgaat tattcctaaa
     4441 tgccggaaat gtggaggaag ttgcgatcag tgcttccaac aaagaatacc gcgaccggat
     4501 tgtagccaga gtggagaatg gtattttaaa aatatactat gagtccaagt tgaaatccat
     4561 aaacaaaaag aaggagagta aacaattaaa ggcctatgtt tcttacaaac aactcgatga
     4621 attacaggcc aacaccggag ccctggtaag atttcaaggg gtgttgaaaa cggtggcctt
     4681 ggatatgaaa gtaaataccg gtgcccaggt gatgggtgaa gtcgatatca aatcgctggt
     4741 agttaaacaa aacaccggat cacaggtaag cctgacgggt aaaacggagc aactgaaagt
     4801 ggatggggat acgggcagca aattcaaggg agaggaaatg accacacagt cctgtgatgt
     4861 tactgtaagt accggggcga atgtgaccat tgatgccgaa aaagaaatta tggtgaaagc
     4921 cagtaccggt gggatcgtaa agtataaagg gaatgcggct atccgtgaaa taaaaaccag
     4981 cacgggtggg acagtaaaaa agattggtta aaccctgtaa ttttataagt atgaaaaaaa
     5041 tattttcaac cctggccatt cttgttttgt catttactgt gcaggcacag atcaatgatg
     5101 cgaatgccca ggtgcgtacg gccagcggct atcatggcat tcatgtttcc agtgctttta
     5161 atgtttacct gagtcagagt aatgaagaag cggtgg
//
LOCUS       DAOPMW010000235         5161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18702_length_5161_cov_4.725225, whole genome shotgun sequence.
ACCESSION   DAOPMW010000235 DAOPMW010000000
VERSION     DAOPMW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18702_length_5161_cov_4.725225"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLU11_10845"
     CDS             <1..587
                     /locus_tag="PLU11_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPN59664.1"
                     /translation="FKNSWAIPGGFVLDKESLEEAVRRELQEETGITVHYLEQLYTFG
                     EPDRDPRQRVVSVAYFGLVKTSQYNELKASTDAENAAWFSIQKLPVLAFDHKQILAIA
                     IERLRTKIRYQPIGFELLDKKFPFSDLEKLYMALLGRDINRRNFSKKILSFGFLEETG
                     ELSRSVGKGRPSKMFQFNQKRYRELLKEGYHFEI"
     gene            complement(588..1517)
                     /locus_tag="PLU11_10850"
     CDS             complement(588..1517)
                     /locus_tag="PLU11_10850"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HPN59665.1"
                     /translation="MGLFDFLFGRAEKSKPAQTPASKSAKKPQAELDYSRELITKEIA
                     PGISLPAAFESQWSQIKGTAIHTIQIKANVSENLTLAQSKFGHFPMMPAGADYPKDSQ
                     GAFMYPLAQLNFSEIPELEGYPRSGYLQFYISITDDAFGMDFENPQSQVNFRVLFFED
                     EEVKDFRSDFAFLEETLRSDMSPVYKPHVLQFEKKTDYLGAADFRSSFHPAFNLTRFI
                     AAYPEDVADELATAAWDTFGCNGHKIGGYAYFTQSDPREKEEESGKYILLFQLDTDDE
                     IMWGDCGVANFFIHPDDLAKKDFSKVLFNWDCC"
     gene            1642..2451
                     /locus_tag="PLU11_10855"
     CDS             1642..2451
                     /locus_tag="PLU11_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HPN59666.1"
                     /translation="MKTINLHTGTHTRFTLFPDNQPHVTIMGVEAGEEVQVIADLTNS
                     VKLIQLLQVANAIGHAGAVKKQLVIPYLLGARYDRIMQPGDSFDLEVIAGMINSCGFL
                     EVLLFDVHSDIAPSLIQHATAISNKRMVQAYKEKNAVLICPDNGAAKKTGEYRDWNSG
                     ISDIVYCEKKRDLSTGKITLKVTEPEKCLGRSCVIIDDICDGGATFLAIADQVSPSDI
                     TLVVSHGIFSKGFALLEQKFDRIICSSSLGQVYQHPIVQTIDIDWTSLKTL"
     gene            2453..3913
                     /locus_tag="PLU11_10860"
     CDS             2453..3913
                     /locus_tag="PLU11_10860"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006629.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPN59667.1"
                     /translation="MKTNPFLLTDYYKVGHVFQYPENTTLVYSNLTPRKSRLNGVDEM
                     VFFGLQYFIKEYLQDYFNENFFNQPKDTVMAAYKRRIQTSLGAPLPSYKHLEDLHDLG
                     YLPVEIKALPEGSKVPMRVPCLTIENTKPEFYWLTNFLESLMSAITWQPSTSATIAWQ
                     YRKLLNRYAVETGMPADFIQWQGHDFSFRGMSSLESAVLSGMGHLLSFSGTDSIPAID
                     ALEKYYGADADKELIGGSVAATEHSVMCSGSQDGELETFRRLITEVYQDGIVSIVSDT
                     WDLWKVCTDYLSALKETVLNRNGKVVIRPDSGDPVKIICGNPEGKTIAEQKGVVELLW
                     DIFGGTITDKGYKFLDSHIGAIYGDSINLERAEAICEGLKLKGFASQVVFGIGSFTYQ
                     YNTRDTFGLAMKATYVEIGGEGRAIFKKPVTDDGTKISATGLLQVIEENGKYVLRDHV
                     SREEEAQSALKTVFKDGKLIREFTLSEIRERINSHQ"
     gene            3947..4486
                     /locus_tag="PLU11_10865"
     CDS             3947..4486
                     /locus_tag="PLU11_10865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HPN59668.1"
                     /translation="MEKNLKHLSKFISLVLRHKPEEIGLLLDANGWADTEELIRKINE
                     KGGGLTLSLLEEIVETNDKKRFAFNEDKSRIRASQGHSIDIELGLEPVQPPEILYHGT
                     ADKNLPSILENGLASQSRQHVHLSATTDTARQVGTRHGKPVVLTIDAAAMYRDGHVFF
                     LSANKVWLTHSVPLKYIAV"
     gene            4483..>5161
                     /locus_tag="PLU11_10870"
     CDS             4483..>5161
                     /locus_tag="PLU11_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN59669.1"
                     /translation="MKRTLVIGDIHGGLRALKQVLQRANVTLDDRLIFLGDLVDGWSE
                     SAQVVDYVMELALQQECIFIKGNHDEWCEDWLRTGQADSRWLPHGGMETLSSYDGYKE
                     ETKNKHLAFIAGMNYYEIDLENRLFIHAGFTSMHGPHAEFHASNFSWDRTLWETALTM
                     DKQLTKESPYYPKRLKLFSEIYIGHTPTQYFNCDLPMNGANVWNIDTGAAFKGRLSVM
                     DIHSKEYW"
BASE COUNT         1480 a         1103 c         1281 g         1297 t
ORIGIN      
        1 catttaaaaa cagctgggcc atacccggtg ggtttgtact ggataaggaa tcactggaag
       61 aggcggtgcg aagggagctg caggaagaga caggaatcac cgtgcactac ctggaacaac
      121 tctatacatt cggtgagccg gaccgtgatc cgagacaacg ggttgtatcc gtagcctatt
      181 tcgggcttgt aaaaaccagc cagtataatg aacttaaagc gagcactgat gcggaaaacg
      241 cagcatggtt cagtattcaa aaactaccag tactggcatt cgatcacaaa cagattttag
      301 caatagctat tgaacggttg cggaccaaga tccgctacca gccaattggt tttgagctgc
      361 tcgataagaa attccctttc tccgaccttg agaaattata tatggcattg ctgggccggg
      421 atatcaaccg gcgtaatttt agtaagaaaa ttctttcttt tggctttctc gaagaaaccg
      481 gcgaactctc caggtcagta gggaagggcc ggccaagtaa aatgttccag tttaatcaga
      541 aacggtaccg agaattactg aaagagggct atcatttcga gatctgatca acagcaatcc
      601 cagttaaaaa gcacttttga aaaatctttt ttagcgagat catccgggtg aataaaaaaa
      661 ttagccacgc cgcaatcccc ccacattatt tcatcatcgg tatctaattg aaacagcagg
      721 atatatttac cgctttcttc ctctttctcc cttgggtctg attgtgtgaa atacgcgtag
      781 cctccgattt tatggccatt acaaccaaat gtatcccagg cggccgttgc cagttcatct
      841 gcaacatctt ccgggtaagc agcgatgaac cgggtaagat taaatgccgg gtggaagctg
      901 ctccggaagt ctgcagcacc caggtagtct gtttttttct cgaattggag gacatgaggt
      961 ttgtacacag gtgacatgtc tgaacgtagc gtttcctcga ggaaggcgaa gtcggatctg
     1021 aagtctttaa cttcttcatc ttcaaaaaac aatacacgga aattgacctg tgattgtgga
     1081 ttttcaaaat ccataccgaa tgcgtcgtca gtaattgaga tataaaactg gagataaccc
     1141 gaacgaggat acccttccaa ctccggaatt tcactgaaat ttaattgggc cagcgggtac
     1201 ataaaggcac cctggctgtc tttggggtag tcagcaccag caggcatcat ggggaaatgc
     1261 ccgaatttac tttgtgccag cgtcaggttt tcagacacat tagcttttat ctgaatagtg
     1321 tgtatggcag tacccttgat ttgtgaccac tgactttcaa aggcggcagg taaggatatg
     1381 cccggtgcga tttcctttgt aataagttca cggctgtaat ccagttcagc ttgcggtttt
     1441 ttagcggatt tggaagccgg tgtctgggca ggtttacttt tttcagcccg gccaaacaga
     1501 aaatcaaata agcccatttt tttaatttaa agctacaaaa gccgcggcga acctggaaaa
     1561 aaataaaatg cttcaaaatg aagcaaaatt tggatatccg ggattttgaa tttaaatttg
     1621 cttataatta aagcaaaata aatgaaaacg atcaatctcc ataccggcac acatacccgg
     1681 ttcacgcttt tccccgataa ccagccgcat gtaaccatca tgggcgtaga agcaggggag
     1741 gaagtgcagg tgattgcgga cctgaccaat tctgtaaaac tgatacaact gctccaggtg
     1801 gccaatgcga tcggtcatgc cggcgctgta aaaaagcaac tggtgattcc ctacctgctc
     1861 ggtgcccgct acgaccggat catgcaacca ggcgacagtt ttgatctcga agtgattgcc
     1921 gggatgatca acagttgtgg cttcctggaa gtactgctgt ttgatgtgca ctctgatatt
     1981 gccccgagcc tgattcagca cgccactgcc atatccaata agagaatggt gcaggcatat
     2041 aaagaaaaaa atgccgtact gatctgtccg gataacgggg cagcgaagaa aacgggtgag
     2101 taccgtgact ggaatagcgg gatcagtgat attgtgtatt gtgagaaaaa aagggacctg
     2161 tcaaccggta aaataacact caaagtgaca gaacccgaaa aatgcctggg aagatcttgt
     2221 gtgatcattg atgatatctg tgacgggggc gccactttcc tggctattgc ggatcaggtc
     2281 agtccttctg acatcacctt agtggtttcg cacgggatct tctccaaagg ttttgcatta
     2341 ctggaacaaa aatttgaccg gatcatctgc agtagcagtc tgggccaggt ctatcagcat
     2401 ccaatcgttc aaacaatcga cattgactgg acttctttaa aaacacttta atatgaaaac
     2461 gaatcctttt ttactgactg attattacaa agtgggccac gtgtttcaat acccggagaa
     2521 taccaccttg gtatacagta acctgacacc gcggaaaagc cggttgaatg gcgtggatga
     2581 aatggttttt ttcggactgc agtattttat caaagaatac ctgcaagact actttaatga
     2641 aaattttttc aaccaaccca aggacacggt gatggcggcc tataagcgcc ggattcaaac
     2701 ttcactgggg gcacctcttc cttcgtataa gcacctggaa gacctgcatg acctgggtta
     2761 cctgccggtt gagatcaagg ccttgcccga ggggagtaaa gtaccgatga gggtgccctg
     2821 cctgaccata gaaaatacaa agccggaatt ttactggctc actaatttcc tggaaagcct
     2881 gatgtccgct attacctggc aaccttccac cagtgctacc attgcctggc aataccgtaa
     2941 actactcaac cgttacgcgg ttgaaacagg aatgccggct gattttatcc aatggcaggg
     3001 acatgatttc agtttcaggg gcatgagcag cctggaatcc gccgtactca gtggaatggg
     3061 gcacctgctc tctttttcgg gaacggatag tattcctgcc attgatgcgc tggaaaagta
     3121 ttatggtgcg gatgcggata aggaactgat cgggggaagt gtggcagcaa ccgaacacag
     3181 tgtaatgtgc agtggcagcc aggatgggga gttggaaacc ttccgccggc tgatcaccga
     3241 agtttatcag gatggcattg taagtattgt cagtgatacc tgggacctgt ggaaagtctg
     3301 tacagattat ttatctgcat tgaaagaaac agtacttaac cggaatggga aagtggtgat
     3361 tcgtcctgac agcggtgacc cggtaaaaat tatctgtggg aatccggaag ggaaaacaat
     3421 cgccgaacag aaaggggtgg tggaattgct ctgggatatt tttggcggca ccattacgga
     3481 taaaggatat aagtttctgg atagccatat cggggccatc tatggagata gtattaatct
     3541 cgaacgggcg gaagcgattt gtgaaggatt gaaattaaaa ggttttgcca gccaggtggt
     3601 ctttggaatc ggtagtttta cttaccagta caatacccgt gatacatttg gtcttgccat
     3661 gaaagccact tatgtagaaa tcggcggcga aggcagggct atttttaaaa aaccagtcac
     3721 cgatgatggt accaagattt ctgccacagg tctcttgcag gtgattgaag aaaatggtaa
     3781 atatgtactc cgggatcatg tcagccggga agaagaagcc caatcagctt tgaaaacagt
     3841 ctttaaagat ggaaagctga tcagggaatt taccttgtcc gagatcaggg aaagaataaa
     3901 ttctcatcaa tgaataatat gaggcagtga ctataacttt tcaacaatgg aaaaaaatct
     3961 aaagcatctc agcaaattca tcagtctcgt tctccggcat aagccggaag aaatcggact
     4021 gttgctggat gccaatggct gggccgatac ggaggagctg atccggaaaa tcaatgaaaa
     4081 aggagggggg ctgacccttt ctttacttga agaaattgtt gagaccaacg ataagaagcg
     4141 gtttgccttc aatgaggata aatcccgcat ccgggccagc cagggacata gcattgatat
     4201 cgaactcgga ctggagcccg tgcagcctcc tgaaatactg tatcatggga cagcagataa
     4261 aaatctgcct tccattctgg agaatggttt ggccagtcaa agccggcaac atgtacacct
     4321 gagtgccaca accgatactg ccagacaggt cggtacccgt catggaaaac cggtggtgct
     4381 gaccattgat gccgccgcga tgtataggga cggacatgtc ttctttctgt cggccaataa
     4441 agtctggctg acccattccg taccattgaa atatattgca gtatgaaaag aacactagtc
     4501 attggcgaca tacatggagg tttaagggca ttgaagcagg tattgcaaag agccaatgta
     4561 actctcgatg accggctgat attcctgggc gacctggtgg acggctggag tgaatcggcc
     4621 caggtggtgg attatgtgat ggaactggca ctacaacagg aatgtatttt catcaaagga
     4681 aatcatgatg aatggtgtga ggactggttg cgtaccggac aggcagacag ccgttggctc
     4741 cctcatggcg gtatggaaac gctcagcagt tatgatggat ataaggagga aacaaagaat
     4801 aaacatcttg cctttattgc agggatgaat tattatgaaa tcgacctgga aaaccgcctc
     4861 tttattcatg ccggttttac atccatgcat ggtccgcacg cagagtttca tgcgtctaat
     4921 ttcagctggg accgaaccct gtgggaaaca gcactgacca tggataaaca attgactaaa
     4981 gaatcaccgt attatccaaa acggctgaaa ttattcagtg aaatttatat cgggcatact
     5041 cccacccagt atttcaactg tgatttgccg atgaacggag ccaatgtctg gaatatcgat
     5101 accggtgccg cgtttaaagg aaggttgtct gtcatggaca tacacagcaa agagtattgg
     5161 c
//
LOCUS       DAOPMW010000236         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_18752_length_5152_cov_5.108888, whole genome shotgun sequence.
ACCESSION   DAOPMW010000236 DAOPMW010000000
VERSION     DAOPMW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18752_length_5152_cov_5.108888"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PLU11_10875"
     CDS             <1..173
                     /locus_tag="PLU11_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HPN59670.1"
                     /translation="WKGYRRGDAGCHERQALVLVNYGRALGSEIFQLSEDILQSVHQK
                     FGVELEREVNII"
     gene            209..685
                     /locus_tag="PLU11_10880"
     CDS             209..685
                     /locus_tag="PLU11_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59671.1"
                     /translation="MKKNIYLFLFLLSTTGSAGQSHQAIYTELGKNGIIIHAGYDQQF
                     INHHTGFRVGIGSNFDRYLKAYAITAGVYKLAGRKYHFFETGLDLQYLNIQENSDDQR
                     GFGFIYPDYTVAAFYPSLNLGYRYSGQKFLFRAGCSPGIIKKEFIPGAYFTFGFCW"
     gene            816..1298
                     /locus_tag="PLU11_10885"
     CDS             816..1298
                     /locus_tag="PLU11_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59672.1"
                     /translation="MRKLIILAMVSIFSSQLMQAQSAYVELGGPGLASINFDTRFTSK
                     DDGIGGRVGVGGFSISDGNGSNAGVVFIPLGVNYLFGKDERNYFEVGGGITPVLGTGN
                     SGSNFSETFGHILFGYRMQPKMGGFTFRAFICPIFASGTFIPYYGGLSFGYKFNAKKE
                     "
     gene            complement(1357..2883)
                     /locus_tag="PLU11_10890"
     CDS             complement(1357..2883)
                     /locus_tag="PLU11_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HPN59673.1"
                     /translation="MNIQNIKTLGELKASGYQSKPIKEEIRANLLKRLKSHEPTFTGI
                     VGYEDTVIPDVERALLSRHNILFLGLRGQAKTRMARQMTELLDEYIPSIAGSDINDDP
                     INPISRFAQEEIATHGDETAIHWIHRSERYGEKLATPDVSVADLIGDIDPIKAANLKL
                     SFADDRVIHYGIIPRSNRGIFVINELPDLQARIQVALFNILEEGDVQIRGFKLRLPLD
                     ILFVFTANPEDYTNRGAIVTPLKDRIQSQILTHYPKNLEHALEITALEADLSAEQQAR
                     VDISDLVKRLIEQVAFEARSSELVDKKSGVSARLTISALENAVSSAERRAIMNNEKQT
                     QVWISDLAGIIPSITGKVELVYEGEQEGPFQVAMNLVDKAIRTQFIQYFPNPDQLKKR
                     KNTGKPSQELIKEDNPYRPITAWFDKGNHLNIDFNTTDKEKIMLLYQVDGLHSLVKKH
                     FPGTNEEQSALLMEFVLHGLASYSLISKKMFESKVEFKDLMGSMMDFNEKDFEFDEEA
                     "
     gene            complement(3061..4158)
                     /locus_tag="PLU11_10895"
     CDS             complement(3061..4158)
                     /locus_tag="PLU11_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPN59674.1"
                     /translation="MRGFHFTRFDPDKDGKSRFEQLLDLFMQLLTYTSGDVTEAMQWL
                     NELDKKYQLTGNEYGMGDFIEDLKQKGYITDPDQQGQIKITPKTEQGIRKKSLEEIFG
                     KLKKTKQGDHHSFKPGQGDEVNPETRQFQFGDMLEQIDFTESIRNAQINHGIDSFHMR
                     EDDLSIRETDYKSQTSTVLMIDISHSMILYGEDRITPAKKVAMALSELIKTRYPKDTL
                     DIVVFGNDAWPIEVKDLPYLQVGPYHTNTVAGLELAMDILRRRKNPNKQIFMITDGKP
                     TCLKIGKRYYKNSFGLDRKVINRCLNLAAQCKKLKIPITTFMIATDPYLQSFVQEFTE
                     MNHGKAFFASLDKLGAFIFKDFESGKRKTVY"
     gene            complement(4282..>5152)
                     /locus_tag="PLU11_10900"
     CDS             complement(4282..>5152)
                     /locus_tag="PLU11_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPN59675.1"
                     /translation="EYLLKTGAVQPGDSILPEFRFELPKRAMMKNDLIILNILAANKW
                     KRPIYFTSPMGELGFGPFMRKEGIAYRLLPVMPKMPQMNWVVDQSLRSKGLGGTQLRD
                     MNLDTTFNNLMTKYEFGGANIKGVYFDEENRRHILNLRSIYGEAAGNMADAGKLDQAK
                     QLIDKVEAGINTANLPYAMTSRYNGHNQTALIYLEACFKAGKKELAEKVAAEIRKDLE
                     QQARYYTYIRDSKPDFWGYYERSEAPINERLLVVLSDIEARYKSAAGSKPVNELNTPS
                     IQTPAKPDSNKQN"
BASE COUNT         1311 a         1115 c         1144 g         1582 t
ORIGIN      
        1 gctggaaggg ataccggaga ggggatgccg gttgtcatga acgccaggcc ctcgtactgg
       61 ttaattatgg aagggcactg ggcagcgaaa tatttcagct cagtgaagat atactgcaat
      121 ctgtacatca gaaattcggg gtggagttgg aacgggaagt aaatatcatc tgatgcaacg
      181 aacttcataa aacagggttc ataccaatat gaaaaaaaat atttacctgt ttcttttttt
      241 actcagcaca accggatctg ccggacaatc acatcaggct atttatacag aattaggaaa
      301 aaacggaata atcatacatg ccggatatga ccagcagttt atcaaccatc atacgggttt
      361 ccgggttggt attggatcca attttgaccg gtacctgaag gcttatgcaa ttactgcggg
      421 tgtatataaa ctggccggaa gaaaatatca ttttttcgag accggactgg accttcaata
      481 ccttaacatt caagagaaca gtgatgatca gcggggattt ggctttattt atcctgatta
      541 tacggttgcg gcattttatc cctctttgaa cctgggttac cggtattcgg gacagaaatt
      601 cctgttcaga gctggctgtt caccgggaat tattaaaaaa gaatttattc ccggagccta
      661 ttttactttt ggcttttgct ggtaaatgat acgtacatgt tttaaacctg taacatttag
      721 cacattacaa tcgtcaaaag ggcgttaata aaatatcccg atgttattat ttaaaaaata
      781 aaatagttac tttaacagca tcaaaaacta ctaatatgcg taaactcatt attctcgcta
      841 tggtatccat tttcagcagc cagctcatgc aggctcagtc cgcttatgtg gagttgggag
      901 gccccggtct cgcatccatc aactttgaca cgaggtttac ttctaaagat gatggcattg
      961 gaggaagagt aggtgtcggt gggttcagta tcagtgatgg aaacggaagt aatgccggtg
     1021 tggtatttat tccactgggg gtaaactacc tgtttggcaa agatgagcgt aactatttcg
     1081 aagtaggagg aggcatcacc ccggtactgg gtacgggcaa tagtggcagt aatttttctg
     1141 aaacctttgg gcacatatta ttcggataca gaatgcagcc taaaatgggc ggattcactt
     1201 tccgggcttt tatctgtccc atttttgcca gtggaacttt tattccatat tacggtggat
     1261 tgtctttcgg atataaattc aatgcgaaaa aggagtaaag tattttttta tatagtgtaa
     1321 atggccggaa caatcagttc cggccatttt ttattctcag gcttcttcat caaattcaaa
     1381 gtctttttca ttgaaatcca tcatgctgcc catcaggtct ttgaattcca ctttggattc
     1441 aaacattttt ttactgatga gggaatagga agcgagtcca tgtaaaacga actccatcag
     1501 cagggccgac tgttcttcat tggtgccggg aaaatgcttc tttaccagtg aatgcaggcc
     1561 atctacctgg tacaaaagca tgattttttc cttatccgtg gtattgaaat caatattcag
     1621 gtgattgccc ttatcaaacc aggccgtaat cgggcggtat ggattatcct ctttgattag
     1681 ttcctgtgat ggttttccgg tgttttttct ctttttgagt tggtcaggat tgggaaagta
     1741 ctggataaac tgggtacgga tggctttatc tacgaggttc atcgccacct ggaaagggcc
     1801 ttcctgttcg ccttcatata ccagttccac tttaccggtt atggaaggta tgatacctgc
     1861 caggtcactg atccagacct gggtctgctt ttcattattc ataatagctc tgcgttcggc
     1921 ggagcttact gcattttcaa gtgctgaaat cgtcagacgg gcactgaccc cactcttctt
     1981 atcgaccagt tcactgctcc ttgcttcaaa agctacctgt tcgatcagtc tttttacaag
     2041 atcacttatg tctacccgtg cctgctgctc tgcactcaga tcggcttcca gtgcggtaat
     2101 ctccaacgca tgttcaaggt tctttgggta gtgggtcagt atctggcttt gaatccggtc
     2161 ctttaaaggc gtaacgatgg ccccccggtt agtatagtct tccggatttg ccgtaaatac
     2221 aaataatata tcaagcggta aacgaagttt gaaaccgcgg atctgaacgt ctccttcttc
     2281 taatatatta aacaaagcaa cctgtatcct ggcttgtaaa tcaggcagtt cgtttattac
     2341 aaatatgcca cgattactgc ggggtataat accgtaatgg atcacccggt catcggcaaa
     2401 actgagtttg aggttggcgg ctttgatcgg atcaatgtcc ccgatcaggt cagccacact
     2461 cacatcgggc gtggctagtt tttcgccata tcgttctgag cggtgtatcc aatggatggc
     2521 ggtttcgtct ccatgagtgg caatttcttc ctgggcaaac cgggaaatgg ggttgatcgg
     2581 atcatcatta atatcactac ctgcaataga agggatatat tcgtccagca attcggtcat
     2641 ctggcgggcc attcttgttt tggcttgtcc gcgtaaaccg agaaacagga tattatgccg
     2701 gctcaataat gcccgctcca catccggaat tacagtgtct tcatatccga caattccggt
     2761 aaaagtgggt tcgtgggatt ttaaccgttt caggagattc gcacgtatct cttctttaat
     2821 tggtttcgat tgataaccgg aggctttgag ttcaccaagg gttttgatat tttggatatt
     2881 catgttagta gtaattaaaa attaaaaagt aaaaagtaaa aaaagggtca gtttttcgca
     2941 tatgcattaa aggaaagggc atttataatc tataatattc tgagtacagg aggccttaat
     3001 tgtttatccg tataaggatt tttacttttt acttttgaat ttttactttg ataagtttct
     3061 tcaataaacc gtcttccgct tcccgctttc aaaatcctta aaaataaacg ctcccagttt
     3121 atccagcgaa gcaaaaaacg cttttccatg attcatctct gtaaattcct gtacaaaact
     3181 ttgcaggtaa ggatcagtgg cgatcataaa agtggtgatc ggaatcttca gttttttgca
     3241 ttgtgccgcg aggttcaggc agcggttgat cactttacga tccagaccaa aactgttttt
     3301 atagtagcgt tttccaatct tcagacaggt gggtttccca tcggtgatca tgaagatctg
     3361 tttattcgga tttttccggc gccttaaaat atccatcgcc agttccaaac cggccaccgt
     3421 gttggtatga tagggaccta cctgcagata aggaagatct ttaacctcga ttggccaggc
     3481 atcattacca aataccacaa tatcgagtgt gtctttcggg taacgggttt tgatcagttc
     3541 gctcagcgcc atagccactt tcttggcagg tgtgatccgg tcctctccat acaggatcat
     3601 actatgtgaa atatcgatca tcagtacggt ggaggtctgc gatttgtaat ctgtttcccg
     3661 gatactcagg tcgtcttccc gcatatggaa actgtcgata ccatggttga tctgggcatt
     3721 ccgtatactt tcagtaaaat caatctgctc gagcatatcc ccaaactgaa actgccgggt
     3781 ctcgggattt acttcatctc cctgtccggg tttaaaacta tggtgatcac cctgtttggt
     3841 ttttttgagt ttgccgaata tctcttccag gctttttttc cggatgccct gttcggtttt
     3901 cggtgtgatt tttatctgac cttgctgatc aggatcggtg atatacccct tctgtttcag
     3961 gtcttcaata aaatcaccca ttccatattc gttaccggtg agctggtact ttttatccag
     4021 ttcgttcagc cattgcatgg cttccgtcac atccccgctg gtgtaggtca gcaattgcat
     4081 aaaaaggtca agcaattgct caaagcggct tttgccatcc ttatcaggat caaaacgggt
     4141 gaaatgaaaa cctctcatcg gatatacaat ttacgacaat tcgggaaggg gagatacatg
     4201 cttgtaacaa ccgggccgcc ggtaagttca tataaaagaa aaccctccgg tccggagggt
     4261 tttctaaagc gtaacttaaa attaattctg tttattactg tctggtttgg ccggggtctg
     4321 gatactgggt gtattcagtt cattgaccgg tttgctaccg gctgcacttt tataccgggc
     4381 ttcgatatca ctcagaacta ccagtaaacg ttcattgatc ggggcttctg agcgctcata
     4441 atacccccag aaatcgggtt tgctatcccg gatataggta taataacggg cctgttgttc
     4501 caggtctttc ctgatctcgg cggcaacttt ttctgccagc tctttcttac ccgccttaaa
     4561 acaggcttcc aggtagatca gggcggtctg gttatggcca ttataacggc tcgtcattgc
     4621 ataaggcaga ttagcggtat tgataccggc ttccactttg tcgatcagtt gtttggcctg
     4681 atccagttta ccggcatctg ccatattacc cgcggcttcc ccataaatgc tgcggagatt
     4741 gagtatatga cgacgatttt cttcatcgaa ataaaccccc ttaatattag ctcccccgaa
     4801 ttcatacttg gtcatcaggt tgttgaacgt ggtatccagg ttcatatctc tgagctgtgt
     4861 gccgccaagt cctttgctgc gtaaagactg gtctaccacc cagttcatct ggggcatttt
     4921 tggcatcacc ggtaataaac ggtaggcgat accttccttg cgcataaacg gtccaaaacc
     4981 gagttcaccc attggtgaag taaaataaat agggcgtttc catttattgg cggcaaggat
     5041 gttcaggata atgaggtcat tcttcatcat agcccgtttg ggtaattcaa aacggaactc
     5101 cggtaagata ctgtcaccag gctgcacagc ccctgttttc agcaggtatt ca
//
LOCUS       DAOPMW010000237         5056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19238_length_5056_cov_7.176565, whole genome shotgun sequence.
ACCESSION   DAOPMW010000237 DAOPMW010000000
VERSION     DAOPMW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19238_length_5056_cov_7.176565"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLU11_10905"
     CDS             complement(<1..1232)
                     /locus_tag="PLU11_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN59676.1"
                     /translation="MKNFKHYILAGILLATTFQITAQGYKLPAYEKFKLPNGLVIYLM
                     EQHEVPLISVSAIFPAGAIYDGEQSGLASLTAAGLKHGTQSMTKAQLDEELDFLGASV
                     NTFASKESSGLSAKFSAKDKDKILSLIKEILLNPVFDSTEFAKEKRRLLVSLEQQKES
                     PRSVIGAYFDKFLYGEHVYGNIVQGKISTVTPLTAEDLKKFYTANYHPDGSAISVVGD
                     FNAKEMKAKLTALFASWKKGPKTAGANPASMAINKPSENRVLLVNKEDAKETTFFIGA
                     PGIARNNPDYVAIDVVNTLFGGRFTSMLNDELRVNSGLTYGAGSRFSALKNGGSFIIS
                     TFTAGKTTEPAIDKTLEVINKLHSNGIDEKSLTSAKNYVKGQFPPRFETASQLAGLMT
                     QMFWYNFDESFINNFEKNV"
     gene            complement(1237..>2551)
                     /locus_tag="PLU11_10910"
     CDS             complement(1237..>2551)
                     /locus_tag="PLU11_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN59677.1"
                     /translation="MKKLFFPALLLLGFITQAQMKADDVKTFTLKNGMKFLVVEDFSI
                     PNANMYLFYKVGSRNEHQGITGLSHFFEHMMFNGAKKYGPKLFDQTMEFNGGANNAYT
                     TENVTVYTNWFPASATEVIFDLEGDRISSLSIDPKMVESERGVVLSERSTGLENSPWE
                     QLSQTLGGVAFLEHPYHWPVIGYEDDMKNWKQEDLERYFKTYYAPNNCVVVITGGIKL
                     EEVKKLAEKYLEPIPAQPAPPKVNIVEPEQTGERRVLVQKEAATPYLMIAYHVPEAKH
                     EDYYALNILNSILSSGKSSRLYTTLVDQQQLATSVFSGFGESFDPTLFQIYGITAKGV
                     NEAALEKGIYEVLEKIKNEGITETELQKIKNQKLMEFYDQVETINGKSNNIGTYEVFF
                     GDYRKMFDAPAAFNKVTAADVQRVAKKYFTKSNRTVGVLKSNTEE"
     assembly_gap    2552..2651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2873..3775)
                     /locus_tag="PLU11_10915"
     CDS             complement(2873..3775)
                     /locus_tag="PLU11_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59678.1"
                     /translation="MKKISLLITLLCAGIIVTAQNNFMIYSFKGNVTLIENATENKVK
                     VGKTMNSSATIKIPAGGVATIVCNEATMFTINKPGSYPLSKFGDSCKVNHSSFTSNYI
                     KYVWNQMTKINGSPGSNRKAFMNTVGAVSRSINNIWIDPRLDTVNYSGVAGTFPLSWK
                     SYAEAKEFNFSLYTADNSTTAVYSTTVTKLKIPLTAFVSKLKPGTSYHWVSAIKGEEN
                     DERKVLNFVSKESFAALLETLKTQGAQFEAPAEQAYRLGFMLEDAHYLAEALDYYAQA
                     AKLDPTNVLYRSTLMSFKKDYEVK"
     gene            complement(3803..>5056)
                     /locus_tag="PLU11_10920"
     CDS             complement(3803..>5056)
                     /locus_tag="PLU11_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPN59679.1"
                     /translation="YLYKRDTLFATIWVFVFIVVLGSIPINFYALNPLKLGLKDFDFH
                     DMAYSKLDVHKDTLDRQITIINIGKADREGLSMLIEKTAALAPKVMGLDVLFEGPRAP
                     YQDSLLASTIARHPQLILASKLDWNDDNDPYKENFFKTNTNAEGYVNMLAEDYATVRL
                     WSPFFENEQNLQADPYSSFDLAILKKYNDTAYQRQVKRKKTDEIINYTRRNNQYRVIQ
                     PENLLNGEVEADWIRGKLVLFGYVNDHPDDIEDKKFTPMNKRFYGKTIPDMNGIVVHA
                     NILSMALENNYIRKLPGWFNWLFAVLIGWIHMALFIKYYLDHHIWFHLVAKIAQVLSA
                     FLFVYAGIWLFENYRIKLDMKYTLYVIIMAVDVIYFYEAWATWMHKKFRYHTVFGHHG
                     HGPEPAHHKEQVLPEPATPPTTDHQ"
BASE COUNT         1236 a         1118 c         1157 g         1445 t
ORIGIN      
        1 acattctttt caaaattatt gataaaggac tcgtcaaaat tgtaccagaa catctgcgtc
       61 ataagtccgg ccagctgact ggcggtttca aaacggggag gaaactgtcc tttgacatag
      121 ttcttagccg aggtcagtga tttttcatct ataccgttgc tatgcagttt gttgattact
      181 tcaagtgttt tatcaatcgc cggttccgtg gtcttacccg cggtaaatgt actgatgata
      241 aaactgcccc catttttcaa cgcactgaaa cggctgccgg caccatacgt caatccgctg
      301 ttgacccgca gctcatcgtt gagcatggaa gtaaaccgtc cgccgaagag ggtattgacc
      361 acatctatgg caacatagtc cgggttatta cgggcaatac cgggcgcgcc aataaagaaa
      421 gtggtttctt tggcatcctc tttattgacc agcagaacgc ggttctccga tggtttattg
      481 atggccattg aagcgggatt ggcacctgct gttttcggtc cttttttcca gctggcaaac
      541 aaggccgtca gtttcgcctt catttctttg gcgttaaaat cacccaccac gctgatggcc
      601 gatccgtccg gatggtaatt agcggtatag aatttcttca ggtcttctgc cgtcagcggg
      661 gtgaccgtag agatttttcc ctgaacgata ttgccgtata catgttcgcc atacagaaat
      721 ttatcaaaat aagcgccgat cacggaacgg gggctttcct tctgctgttc gaggctgacg
      781 agcaaccgtc ttttttcttt agcaaactcg gtgctgtcaa aaaccggatt aagcaggatc
      841 tcttttataa gcgacagtat tttgtccttg tctttggcag aaaattttgc ggagagaccg
      901 gaagattctt tcgaagcgaa tgtattgaca ctggcaccca ggaaatccag ttcctcatcc
      961 agctgggcct tggtcatgga ttgtgtacca tgtttaagtc ccgcggcggt caaagaagcc
     1021 agtcctgatt gctcgccatc ataaatggcg cccgcaggaa agatggctga tacactgatc
     1081 aggggtactt catgttgctc catcaggtaa atcaccagtc cattgggtaa tttaaatttt
     1141 tcgtaagccg gcagtttata tccctgcgct gtaatctgga aagtggtggc cagtaaaatg
     1201 ccggccagta tataatgttt gaaatttttc atggtattat tcctctgtat ttgattttaa
     1261 cacccccacc gtacggttgg atttggtgaa atatttttta gccacccgtt gtacatcagc
     1321 ggcggtgact ttattaaagg ccgcgggcgc atcaaacatt ttacggtagt cgccgaaaaa
     1381 cacttcatag gtcccgatat tattggactt gccgttgatg gtctccacct ggtcgtaaaa
     1441 ctccatcagt ttctggttct tgatcttctg cagttccgtt tcggtaatgc cttcgttctt
     1501 tattttctcc aatacctcat aaatgccctt ttcgagtgcg gcctcgttta cacctttagc
     1561 cgtgattccg tagatctgaa aaagagtggg atcaaaactt tccccaaagc cggaaaagac
     1621 cgaagtcgcc agttgttgct gatccaccaa tgtggtataa agacgggagg attttccact
     1681 ggaaagaatg gaattcagaa tattcaatgc ataatagtcc tcatgttttg cttccggtac
     1741 atggtaggca atcatgagat agggggtggc ggcttctttc tgcaccagta cccgccgttc
     1801 gccggtttgt tccggttcca cgatattcac tttgggaggg gccggttggg cgggaatggg
     1861 ttccaggtat ttctccgcca gttttttcac ttcttccagt ttaatgccac ctgttatcac
     1921 caccacacag ttattggggg catagtaggt tttaaaatag cgttccaggt cttcctgttt
     1981 ccagttcttc atatcatcct catacccgat caccggccaa tggtagggat gttcgaggaa
     2041 ggccacgcca cccaatgttt gcgataattg ctcccaggga gaattctcca atccggtact
     2101 tctttcactg agtaccacgc ctctttcact ttccaccatt ttggggtcga tgctgagact
     2161 ggaaatacga tcgccttcga gatcaaagat cacttccgtg gctgatgcag ggaaccaatt
     2221 ggtatagact gtgacatttt cggttgtata agcattgttg gccccgccgt tgaattccat
     2281 ggtttggtca aacagcttcg ggccatattt tttagcaccg ttaaacatca tgtgttcaaa
     2341 aaaatgggag agtccggtga tgccctggtg ctcgttccgg ctgcccactt tataaaaaag
     2401 atacatattg gcattgggga tggaaaagtc ttccaccacc aggaacttca tcccgttttt
     2461 cagggtaaaa gtctttacgt catcggcctt catttgtgcc tgagtgataa atcccaggag
     2521 caggagggcc ggaaagaata gttttttcat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn ntgcctgagt gataaatccc aggagcagga gggccggaaa gaatagtttt
     2701 ttcatgcaag taaattaggg gataaagcta cggaatgccg gataaagtac gaggttagaa
     2761 gtacgaagta cgaatgagat gccgtaaaag gagtaactgt acggtatatt agattcaacc
     2821 tcctttggag gggctgttga ctactaacta ccgactacta actacgaact agctacttta
     2881 cctcgtagtc tttcttaaac gacatcagcg tcgaccgata cagcacattc gtagggtcca
     2941 gtttggcggc ctgtgcgtaa tagtcgagtg cttcagccag ataatgggca tcttccagca
     3001 taaagccgag ccggtaggcc tgttcagcgg gggcttcaaa ctgggcaccc tgagttttta
     3061 aggtttcgag cagggcggca aagctttcct tgctgacaaa attcaacact ttacgttcgt
     3121 cattctcttc gcctttgata gcggataccc aatggtaact ggtaccgggt ttgagtttgc
     3181 tgacaaaagc agttaaggga attttcagtt tggtaacggt tgtactatac actgctgttg
     3241 tggaattatc agctgtatag aggctaaaat taaattcttt ggcttctgca taggatttcc
     3301 aggacagggg gaacgtaccg gctacacctg aataattcac ggtgtccagg cgcggatcga
     3361 tccagatatt gttgatgctg cggctcacgg cacctactgt attcataaag gccttgcggt
     3421 tactaccggg agaaccgttg atctttgtca tctgattcca cacgtatttg atatagttgg
     3481 atgtaaatga actgtggttc actttgcagg agtctccaaa tttgctgagt ggataactgc
     3541 cgggtttgtt gatggtaaac atcgtggctt cattacatac aatagtggcc accccgccgg
     3601 ccgggatttt aatagtagcc gaactgttca tcgtcttgcc gactttcact ttattctccg
     3661 tggcgttttc aatgagtgtt acattgccct tgaaactata gatcataaaa ttgttctggg
     3721 cggttacgat tatgccagcg caaagcaggg tgattaataa actgatcttt ttcatttgtc
     3781 ttttttattt cggttgagtt tgttattgat ggtctgttgt tggaggggtg gccggctctg
     3841 gcagcacctg ctctttatga tgtgccggtt ccggtccatg gccatgatgc ccgaatacgg
     3901 tgtggtaacg gaatttctta tgcatccagg tagcccaggc ttcatagaag taaatcacat
     3961 ccaccgccat gatgattaca taaagtgtgt atttcatatc cagtttgatc ctatagtttt
     4021 caaaaagcca gattcccgca tacacaaaaa gaaaagcaga gagtacctgg gcgattttag
     4081 ccaccaggtg aaaccagata tgatgatcca ggtaatattt tatgaaaagg gccatgtgga
     4141 tccagccaat cagcacggca aataaccagt tgaaccaccc gggtaatttc cggatataat
     4201 tattttccag cgccatgctg aggatattgg catgtaccac aatcccattc atgtccggga
     4261 tggttttgcc ataaaaccgt ttattcatcg gggtgaactt tttatcctca atatcgtccg
     4321 gatgatcgtt gacatatccg aacaaaacca gttttcccct gatccaatca gcttccactt
     4381 ccccattaag cagattctcg ggctgaataa ccctgtattg attattacgc cgcgtatagt
     4441 tgatgatctc atccgttttt tttctcttta cttgccgttg ataggccgta tcgttatact
     4501 ttttcaggat cgccagatca aaactggaat aggggtccgc ctgaagattt tgctcatttt
     4561 caaaaaaagg agaccataaa cgaaccgtgg cataatcctc ggccagcata tttacatacc
     4621 cctctgcatt ggtattggtt ttaaaaaaat tctctttgta agggtcattg tcgtcattcc
     4681 agtcaagctt tgatgccagt atcaactgtg gatgacgggc gatcgtggag gccagtaatg
     4741 aatcctggta gggggcccgc ggaccttcaa acaaaacatc cagtcccatt acttttggcg
     4801 ctaaggcggc agttttctca atcagcatgg acaggccttc ccggtctgct ttgccgatgt
     4861 taatgatcgt aatttgccgg tcaagggtgt ctttatgaac atccagcttg gaataagcca
     4921 tatcatgaaa gtcaaaatcc ttcagaccca gttttagtgg attcagtgca tagaagttaa
     4981 tcgggatgga accgaggaca actataaaaa caaagaccca gatggtggca aacaaagtat
     5041 cccgcttata caggta
//
LOCUS       DAOPMW010000238         5032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19369_length_5032_cov_6.965039, whole genome shotgun sequence.
ACCESSION   DAOPMW010000238 DAOPMW010000000
VERSION     DAOPMW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19369_length_5032_cov_6.965039"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..590)
                     /locus_tag="PLU11_10925"
     CDS             complement(225..590)
                     /locus_tag="PLU11_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59680.1"
                     /translation="MVQVDISQHSAECNAWRAQLRNYRDELMQDENQIRQIAGKPLSK
                     EQLLEVEHLHNQFHIQLINIHDLKQSIKLHERQLQVESPASEETAGTHEKLQDEFRSL
                     ELTLQQLRSEFSGFLSRTS"
     gene            complement(828..1739)
                     /locus_tag="PLU11_10930"
     CDS             complement(828..1739)
                     /locus_tag="PLU11_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59681.1"
                     /translation="MKAFSKLSTLITGFLFIGASATAQQTGVDSTGLPGDHFSLQGAL
                     QLFQQSTSIEDFEKALNLESNHVNNLDLNGDGETDYIRVIDKSEKEVHAFVLQVPVSA
                     SENQDIAVIELEKTGDSTAVVQIIGDEDIYGEAVIVEPDGGGEEEAFMDYNDSKLSGP
                     NAGYNFMTPRIVINVWAWPSVRFIYGPVYRPWISPWRWKNYPGWWRPWRPFAWQVWHP
                     YRMHYHRPFVVVRTHRVLHAHRVYTPFRTTSVTVRTRHASTVGNYRVNRTRTTVAGPR
                     GNKATIKKTTVTGPRGNKATRVKVRKH"
     gene            1942..3975
                     /gene="dnaG"
                     /locus_tag="PLU11_10935"
     CDS             1942..3975
                     /gene="dnaG"
                     /locus_tag="PLU11_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPN59682.1"
                     /translation="MISPNTIQQILGRTDIIDVVGSFVKLKRRGSNYLGLCPFHNEKS
                     PSFTVSPSKEIYKCFGCGKSGNTVSFVMEHEKYSYVEALKWLANRYGIEIEETFVSDE
                     QRQQLQAADSLYIINSFAQQFFSRMLFESEEGQDIGLAYFKERGFREEIIKKFQLGYS
                     PEQRDAFTKEAIGKQFNPELLVKSGLVANRNDQLMDNYRGRVIFPVHNHSGKVLGFGA
                     RILKANEKAPKYINTPENELYIKSKILYGSYFARQAIDKADECLLVEGYTDVVSLHQA
                     GIENVVASGGTSLTPDQLRLIRKYTNNLTIIYDGDSAGIKAALRGLDLALEEGLNVKL
                     VLIPDKEDPDSYVNKVGAGAFTQFIQKNKKDFILFQLEVALKDAGSDSIKKSEVVNRM
                     AETIARINKAEDFTRQQDYIKQCSELLKIDEGGLHALVNKFIRDRVSSMERKQPAEEA
                     KQFEQNARQGEESNFDETTFNLLFKDDLQEREVARIILEYGIKKWDDQKLVGEYILEE
                     MVDESLLDNKEVIRLIAVFRELLQQRPEQASRSFFIHHPDSNLSAFAVSLLNFPYEES
                     DHWRSEFSQAGGYQKSLFEQSYEDFIRTVSPENNDQLLRFLKIDEDKTNDQVESAVNY
                     LKLRKIKRMLLENQLDLEKEHTPEEFSMLHQTHDHLKQMEIELTRKMGTVIIR"
     gene            complement(3965..4498)
                     /locus_tag="PLU11_10940"
     CDS             complement(3965..4498)
                     /locus_tag="PLU11_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPN59683.1"
                     /translation="MSYLNVEIKARSKDSARIREYLVSQGAELKGTDRQTDTYFNVPN
                     GRLKLREGNIENNLIFYERPDQAGPKQSAFQLVRVPDAAGLKKALASSNGIKVVVEKI
                     REIYYLRNVKFHIDEVPGLGSFVEIEAGNIKAPELDAGQLKAQCDFYLAAFGIAAPDL
                     VEVSYSDLLMAKQGISG"
     gene            complement(4554..5000)
                     /locus_tag="PLU11_10945"
     CDS             complement(4554..5000)
                     /locus_tag="PLU11_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59684.1"
                     /translation="MSQTVFEKLQLADEKNLLIQGLPSSIEKQFSKLSFCKNMTPLLK
                     SRKIDFALVFAVNENQLNGILRDVMPSLKTGSKFWVAYPKTTSKIVTDLNRECSWNGL
                     TCEGYESIERVELDHVWSAMRFTRCEEYRGADIGASRKRAAAAVAE"
BASE COUNT         1378 a         1101 c         1217 g         1336 t
ORIGIN      
        1 atgcctgctt caaacagcat gcactccgct aaggtggtct taccggagcc ggcatggccg
       61 agcaagacaa tattttttac gtgtgaggta tcaaattctg gcatagcaat aaaatttagt
      121 attgaagaaa atgtgtatga cttctacaaa tcttcaaaat tgatcagata gtaacaatga
      181 acccggttag ggtccgggct gattaaagtc agtaatcagt cgcttcagga agttcgcgac
      241 agaaaaccac tgaactcaga acggagctgc tgaagagtaa gttcgagtga gcggaattca
      301 tcctgtaatt tttcatgtgt accggctgtt tcttctgagg cgggggattc aacctgtaat
      361 tggcgttcgt gtagtttgat cgactgcttg agatcgtgaa tgttgatcag ctggatgtga
      421 aactggttgt ggagatgttc gacttcgagt aattgttctt tggagagcgg tttaccggcg
      481 atttgccgga tctggttttc gtcttgcatc aattcatcac gataattgcg gagctgtgct
      541 ctccaggcat tgcactccgc agagtgctgt gagatgtcta cctgtaccat ggcatttaaa
      601 atttagaagt taattaataa aagaactggt tggaaatact gtctgaagct gacagattat
      661 ccagggttat ggacttagca gaggagggtt tttgttcttt ttgccacagt taagttaaga
      721 ccattttaaa taaattcaaa taaaaaacag gcttaaataa aaaaagaaat gcacaggatg
      781 atcctgtgca ttttttccgt atattctatt tattcattgt taaaacatca atgttttctc
      841 acttttaccc gggtggcttt attgccacgc ggaccggtta cggttgtttt tttaatggtg
      901 gctttattgc cgcgcgggcc agccacggtg gtacgggtac ggttgacgcg atagttgcca
      961 accgttgacg catgtcgggt acggacagtt actgaagtgg tacggaacgg cgtgtaaacc
     1021 cggtgtgcat gcaacactct gtgcgtgcga accactacaa aggggcgatg ataatgcatc
     1081 cggtacggat gccagacctg ccaggcaaag ggacgccagg gtctccacca accggggtaa
     1141 tttttccagc gccagggaga aatccaggga cggtaaacgg gcccatatat aaaccgaacc
     1201 gaaggccatg cccatacatt gatcactata cggggcgtca tgaaattata gccggcattc
     1261 ggaccgctca atttactgtc attataatcc atgaaagctt cttcctctcc tcctccatcc
     1321 ggttcaacga tcaccgcttc cccatatata tcttcatccc cgatgatctg aaccaccgcg
     1381 gtgctgtcac ctgttttttc cagttcaatc accgcgatat cctggttctc tgaggcagag
     1441 accggtacct gcaaaacaaa agcatgcacc tctttctctg atttatctat gacacggata
     1501 taatctgttt ccccgtcccc attcaggtcg aggttattga catgattgga ttccaggttc
     1561 agggctttct caaaatcttc gatgctggtg gattgctgaa acaattgcag ggcgccctgc
     1621 aaactgaaat ggtcgccggg taacccggtg gaatcaaccc ccgtttgctg ggcggttgcg
     1681 gaagctccta taaacaggaa tccggtaatc agggtggata atttgctgaa tgctttcatg
     1741 taataagttt tgtatgagac gagaaaagta tccgatggtt tattcaacgt cgggtgtttt
     1801 ttcggaattt atcacatctg agtgatttta aacgattttc agggataaaa tcaagggggt
     1861 aaactgctgt tttcaactgg ccaacccaat tattgacgag tccgggattg tcccgcattt
     1921 ttaataactt gccctccctt catgatttca cccaatacca tacaacaaat actcggacgc
     1981 acggatatta ttgatgtggt aggcagtttt gtcaagctga aaagacgggg ttccaactac
     2041 ctgggccttt gcccttttca caatgaaaaa tcgccctcct tcacagtttc gcccagcaag
     2101 gaaatctata agtgctttgg ctgtggaaaa agcgggaata ccgtcagctt tgtgatggag
     2161 catgaaaagt acagttatgt tgaagccctt aaatggctgg ccaaccggta cggtattgag
     2221 attgaagaaa cctttgtaag cgatgaacaa cggcagcaat tacaggccgc cgacagcctt
     2281 tatatcatca acagttttgc ccagcagttt ttcagccgga tgctatttga gtctgaagaa
     2341 ggccaggata taggactggc ctatttcaag gaacggggat tccgggaaga gatcattaag
     2401 aaattccagt tagggtattc gcccgaacaa cgggatgcat ttacaaaaga ggctattgga
     2461 aaacaattca atccggaact cctggttaaa tccggactcg ttgcgaaccg gaatgatcag
     2521 ttgatggaca attaccgggg acgggtcatc ttcccggtac ataatcatag cggcaaagtg
     2581 ctcgggtttg gtgcccgtat tctcaaggcc aatgagaagg ctcccaagta tatcaatacc
     2641 ccggagaacg aactctatat aaaaagtaag attctttatg gctcctattt tgcccggcag
     2701 gccattgata aagcagacga atgtctgctg gtggaaggtt atacagatgt ggtgtcttta
     2761 caccaggcag gaattgaaaa cgttgttgcc agtgggggta catccctgac ccccgatcag
     2821 ttgcggctga tccggaaata cacgaataac ctcaccatta tttatgatgg ggattccgcg
     2881 ggaataaaag cggcgctgcg ggggctggac ctggcgctgg aagaagggtt gaatgtaaaa
     2941 ctggtgctga taccggataa agaggacccg gacagttatg tcaataaagt gggtgccggg
     3001 gcattcaccc agtttatcca gaaaaataaa aaggacttta tcctcttcca gctggaagtg
     3061 gccctgaaag atgccggttc cgattccatt aagaaatcgg aagtggtgaa ccggatggcg
     3121 gaaaccattg cccggatcaa taaggcggag gattttacca ggcagcagga ttatatcaag
     3181 caatgctccg aactgctgaa aattgatgaa ggaggactgc atgcactggt caataaattt
     3241 atccgtgacc gggtcagcag tatggagcgg aagcaaccgg cagaggaggc aaagcagttt
     3301 gagcaaaatg ccaggcaggg agaagaaagc aattttgatg aaactacttt caacctcctg
     3361 tttaaagatg atctgcagga acgggaagtt gcccggatta tcctggaata cgggatcaaa
     3421 aaatgggatg atcaaaaact ggtgggggaa tatatactgg aggaaatggt agatgaatcc
     3481 ctgcttgata ataaagaagt gatccggctt atcgctgtct tcagggaatt attgcaacaa
     3541 cgtccggaac aggccagccg cagttttttt attcatcatc ctgattccaa tttaagtgct
     3601 tttgccgtat ccctgctcaa ttttccttac gaggaaagtg atcattggcg gagtgaattc
     3661 agtcaggccg gtggctacca gaaaagcctt tttgaacaat cctatgagga ttttatccgg
     3721 acggtttcgc cggaaaacaa tgatcagtta cttcgtttcc tgaaaataga tgaggataag
     3781 accaatgacc aggttgaatc agccgtaaac tacctgaaac tgcggaaaat taaaaggatg
     3841 ctgctggaga atcagctgga cctggaaaaa gaacataccc cggaggaatt cagtatgctg
     3901 caccagaccc atgatcacct gaaacagatg gaaattgaac tgacccgcaa aatgggtact
     3961 gtaattatcc gctgatgccc tgctttgcca tcagcagatc actgtaagat acttccacca
     4021 gatcgggcgc tgcaatacca aaagccgcta aataaaaatc acattgtgct tttaactgcc
     4081 ccgcatccaa ttcaggtgct ttaatatttc cagcttcgat ttccacaaag gaacccaggc
     4141 cgggtacctc gtcaatatgg aatttgacat tccggagata atagatttcc cggatttttt
     4201 ccacgactac ttttatgcca tttgaactgg ccagggcttt tttaagtccg gccgcatcgg
     4261 gtacccgaac cagttggaag gcggattgtt tgggaccagc ctggtccgga cgttcataaa
     4321 aaatcaggtt attctcgata tttccctccc tgagtttcaa ccggccattg gggacattaa
     4381 aataagtgtc ggtctggcgg tctgttcctt ttaattcggc cccctggctg accaggtatt
     4441 cacggatcct ggcggaatcc ttacttcggg cctttatctc cacattcagg taactcataa
     4501 aattaaaaag ggctgcatca tggagataca gccctggaat tcaatttaaa aatttattca
     4561 gctacagcag cagcggctct tttacggctt gcaccgatat cagcgccacg gtactcttcg
     4621 caacgggtaa aacgcattgc actccataca tgatccagtt caacccgttc gatgctttcg
     4681 tacccttcac aggtcagccc gttccagctg cattcccggt tgaggtcggt cacaattttg
     4741 gaagtggtct tgggataagc cacccagaat ttagaaccgg ttttaaggga aggcatcaca
     4801 tcacggagaa taccattcag ctggttttca tttacagcaa atacgagcgc aaagtcaatt
     4861 ttccggcttt ttaaaagcgg cgtcatgttc ttgcagaaag acagcttgct gaattgtttc
     4921 tcgatggagg agggcaaacc ctggattaga agattctttt cgtctgccag ttgtaacttt
     4981 tcaaaaacag tttgtgacat acttagaagc tattttaaag gttccggctg at
//
LOCUS       DAOPMW010000239         5030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19385_length_5030_cov_6.873166, whole genome shotgun sequence.
ACCESSION   DAOPMW010000239 DAOPMW010000000
VERSION     DAOPMW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19385_length_5030_cov_6.873166"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLU11_10950"
     CDS             <1..191
                     /locus_tag="PLU11_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59685.1"
                     /translation="IPLADFNQPKRWRFFACGGPKEVKGEFKEKKEILIFVYRLQTLT
                     NPNDGGFSPAAILKSLEV"
     gene            518..1546
                     /gene="pheS"
                     /locus_tag="PLU11_10955"
     CDS             518..1546
                     /gene="pheS"
                     /locus_tag="PLU11_10955"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPN59686.1"
                     /translation="MDNLLNQIAGYKNEISAFTAADEKQVEEYRIKWLGTKGIVKATM
                     GEMKNVAPEQKKEAGQILNEFKQFVEARYEELKAAATGTVQTGFSIDISLPGDTMPLG
                     SRHPVTLMRNRIVSIFQRLGFAVAEGPEIEDDWHNFGALNLPAHHPARDMQDTFYIQQ
                     NPDWVLRTHTSSVQIREMEKGKLPIRMLMPGRVYRNETVSARSHCFFHQVEGLYIDEN
                     VSFADLKQTLYFFVKEMYGKDVKVRFRPSYFPFTEPSAEMDVTCFICGGSGCNICKKT
                     GWVEILGCGMVHPNVLSNCGIDPNKYTGFAFGMGIERPAMLKYGVNDLRLFSENDVRF
                     LKQFTSAI"
     gene            complement(1731..2609)
                     /locus_tag="PLU11_10960"
     CDS             complement(1731..2609)
                     /locus_tag="PLU11_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59687.1"
                     /translation="MKSIPFLIVFFIQLSLHSQTRERWEQVVNWDGVSHWSRYMITLP
                     AFQGPNSLPVPQLGNGRIDSNSYIGVSGAFHFSKGDNTQNVVINGHLAVVKDVVGVEL
                     TWIPWEHYTMSTAIKEKRHVYYEKFNDNKAAGDLMLNTNFRLLKKWAPVMDLIFQIGY
                     RMPSGTDYGSARYSDGPGYHFAINMGKPLGKAKTVKLTAMAGFYVWQIESDIFKQDDA
                     FLFGAGLEYRKKNFSLHTNIAGYLGYLENQGDKPVVFRTRAEQQFKKMKLVLGIQQGL
                     NDFDYSSFDAGLRFYF"
     gene            complement(2824..3399)
                     /locus_tag="PLU11_10965"
     CDS             complement(2824..3399)
                     /locus_tag="PLU11_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59688.1"
                     /translation="METSQTETPAMPVTPVPQTPGILATKIPATTSFVIAIAVFLMPF
                     LDIRCNEMSLKKINGVELATGYQIDSPGKNNSLLGGLENKETGSFSKNEKKESNIYAM
                     AALLIGAAGLVLSLLNAKTAMAAAAATGALAAVALIGLMLDINRQVSTQQAEQSKDVV
                     ISVNFTVWFYLSVLAFLAAAYFSYQRMRQSK"
     gene            3478..4326
                     /locus_tag="PLU11_10970"
     CDS             3478..4326
                     /locus_tag="PLU11_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPN59689.1"
                     /translation="MEINSICVCGAGTMGSGIAQAAAQSGLATILYELNAAVLEKAKA
                     GIDRNLQSLVEKGKMTAEEKQKVFGLIRFTDDLQTCLADVFIEAIVEKPELKIALFNQ
                     LAELNHGDVIFASNTSSLSITEIAKGVLHPERVIGMHFFNPATIMKLVEVVNTPFTNT
                     ATTETIISLAKQLKKTAVVCIDSPGFIVNRVARPYYIESLRLLEEGHTEKEQLDRLLE
                     ATGFKMGPFKLMDLIGHDINYAVSCSVWEQLGRPERLKPSLLQEEIVKQGKLGKKTGE
                     GFYKYE"
     gene            4382..>5030
                     /locus_tag="PLU11_10975"
     CDS             4382..>5030
                     /locus_tag="PLU11_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN59690.1"
                     /translation="MSKKILVTGGTGFLGSYIIKELVEKNYTVRAIRRGNKLPGWIPA
                     SVLQQVEWVEGDILDVVSLEDAMEGIDTVIHSAAVVSFVKKDRNQMYQVNVEGTANVV
                     NIALEKNIQKLVHISSVAALGRNAMGGHVNEEKKWEDNKINTHYAKSKFKAELHVWRG
                     ISEGLDAVILNPGTILGYGDWHQSSCAIFKQVYEGFKWYTSGINGFVDVQDVSRAT"
BASE COUNT         1322 a         1157 c         1221 g         1330 t
ORIGIN      
        1 gtataccgct tgcagacttt aaccaaccca aacgatggag gtttttcgcc tgcggaggcc
       61 ctaaagaggt caaaggagaa tttaaagaga aaaaagagat cttgattttc gtataccgct
      121 tgcagacttt aaccaaccca aacgatggag gtttttcgcc tgcggcaatc ttaaagagtt
      181 tagaggttta gtcgtttgac gtttaaagtt gtcttttatt atatcactcc ctctttattc
      241 tcctttatct cttttgacct ctttagcgcc ccgcagggcc taataggttt aaaggtttag
      301 tcgtttgacg tttaaagttg tcttttatta tatcactccc tctttattct cctttatctc
      361 ttttgacctc tttagcgccc cgcagggcct tttaggtttt gaggtttaga gaagcatttc
      421 cttatatcac gccctcttaa ttctcctttg cctctttgac ctctttagtg ccccgcaggc
      481 atctcccaat ttcccgctca tcgcttatct ttgcaccatg gacaatttgc tgaaccagat
      541 cgccggatac aagaacgaaa tcagtgcctt cacggctgcc gatgagaagc aggtggagga
      601 ataccgcatc aaatggctgg gtaccaaggg tattgttaag gccaccatgg gggaaatgaa
      661 gaatgtggcc cctgaacaga agaaagaagc cggacagatc ctgaatgaat tcaagcaatt
      721 tgtggaagcc cggtatgaag aactcaaggc cgcagctacc gggacagtac agaccggttt
      781 ttcgattgat atcagtctcc ccggagatac catgcccctg ggttcccgtc acccggtgac
      841 cctgatgcgc aaccggatcg tgtccatctt tcagcgactg ggatttgcgg tggctgaggg
      901 tccggagatc gaggatgact ggcataattt cggtgccctg aacctgccgg ctcatcaccc
      961 ggcccgggac atgcaggata ccttttatat tcagcagaac cccgactggg tgctgcgtac
     1021 gcatacatcc agtgtacaga tccgggaaat ggaaaagggc aaactgccga tccggatgtt
     1081 gatgcccgga cgtgtttacc gtaatgagac ggtgagcgcc cggagccatt gcttctttca
     1141 ccaggtggaa ggactgtata ttgacgaaaa tgtatctttc gctgacctta aacaaacatt
     1201 atacttcttc gtaaaagaga tgtacggcaa ggatgtgaaa gtccgtttcc gtccctctta
     1261 tttccccttc accgagccca gcgccgaaat ggatgtgacc tgttttatct gcggtggcag
     1321 cggctgtaat atctgtaaga aaaccggttg ggtggagatc ctcggttgcg gcatggtgca
     1381 tcccaatgta ctgagcaact gcggcatcga tccgaataaa tacacgggct tcgcctttgg
     1441 catgggcatc gaaagaccgg ccatgctgaa atacggggtg aatgacctgc ggttgttctc
     1501 ggagaatgac gtaaggtttt tgaagcagtt tacttcggcg atttaaatac aaatacattt
     1561 cccgcagatt acgctgattt ttagcgcaga ttttcgcaga ttagaatccg aaagttctga
     1621 tctcattaca gcatcatatt tcccctctag cggtaaatac taaataaaaa aatctgcgca
     1681 aatctgcgtt atcatctgcg taatctgcgg gaaaaatgta ttcaggccta ttagaaatag
     1741 aaccgcaggc cggcatcgaa actgctgtag tcgaaatcat tgagcccctg ctggataccc
     1801 aataccagtt tcatcttttt aaattgttgt tcggcgcggg tgcggaagac caccggttta
     1861 tcgccctggt tttcgagata gccgaggtag ccggcaatat tggtatggag actaaagttc
     1921 tttttccggt attccaaccc ggccccgaat aaaaacgcat catcctgttt gaagatatcg
     1981 gattcaatct gccagacata gaatccggcc atcgcggtga gctttactgt ttttgctttt
     2041 cctagcggct ttcccatatt gatggcaaaa tgataaccgg gaccatcact gtagcgggcc
     2101 gaaccataat cggtgccaga gggcatgcgg tagccgatct ggaaaatcag gtccatcacg
     2161 ggcgcccatt ttttcagcaa cctgaaattg gtattgagca tcagatcccc ggcggccttg
     2221 ttatcattga acttttcata gtacacatgc cgcttttcct tgatggccgt gctcatggta
     2281 taatgctccc agggaatcca ggtcagctcc acccccacca cgtctttcac cacggcgaga
     2341 tgtccattga tcactacatt ctgcgtgtta tctccttttg aaaaatgaaa agccccggat
     2401 accccgatat aagaattact atcaatccgt ccattgccca gttgcggaac cggcagcgag
     2461 ttcggtccct ggaaagccgg cagcgtgatc atataccttg accagtgact caccccatcc
     2521 cagttcacta cttgttccca gcgctcgcgg gtctgggaat gcagggaaag ttgaatgaag
     2581 aagactatca ggaatggtat ggacttcata ttgctttaag aaagttatgt ttcagttgtc
     2641 ggatgtcaga tgtcagttgt caggaaacag cccttactta gaaggttttc ctaaaataac
     2701 gaatcgatac ccgttttacg gcatctcctt gtcaccctga gtagtcccgc taaagtattt
     2761 tttcagggct tatgaaaatt ttcatgcggg acgtatcgaa gggtcgtagt acgtactttg
     2821 tacttattta ctttgcctca tccgttgata cgaaaaataa gcggctgcca ggaatgccag
     2881 gacggaaaga taaaaccaga ctgtgaaatt gacagatatt acaacatcct tactttgctc
     2941 cgcctgttga gtactaacct gccggttgat atccagcatg agtccgatca gcgctacggc
     3001 tgccagtgcc cctgtggcag cagcagcggc cattgccgtt tttgcattca ataaggatag
     3061 taccaggccg gctgcaccga tcagcaaagc agccattgca tatatatttg actccttttt
     3121 ttcattcttt gaaaaactgc cggtttcctt attttcaagc cccccaagca atgaattatt
     3181 ctttccggga ctatctatct ggtaacccgt tgccagttcc acgccgttga tttttttcaa
     3241 tgacatttca ttacagcgga tatccagaaa aggcatcagg aaaacggcta tagcaataac
     3301 aaaactggtc gtggccggaa tttttgtagc cagtataccc ggtgtttgag gcacaggtgt
     3361 tacaggcatt gctggggttt ctgtctgact ggtttccatg attaaaaatt tttttcaagt
     3421 taagctaaag caggcacagt tcagtaaccg gcttatcttt acggaataat ctggtttatg
     3481 gagatcaaca gtatttgcgt ttgcggggcg ggtacgatgg gcagtggcat tgcacaggca
     3541 gcggcccagt cggggttggc cacgatcctg tatgaattga atgcagccgt attggaaaag
     3601 gccaaagccg gtatcgaccg aaacctgcag tcactggtgg agaaaggaaa aatgacggcg
     3661 gaagaaaaac aaaaggtatt cggactgatc cggtttacgg atgacctgca aacctgtctt
     3721 gccgatgtat ttattgaagc cattgtggaa aaacctgaac tgaagattgc tttatttaat
     3781 caactggccg aactcaacca tggagatgta atcttcgcca gcaatacctc ttctctttcc
     3841 attactgaaa ttgccaaagg agtgctgcat cctgaacggg tgatcggtat gcattttttc
     3901 aatccggcta ccattatgaa actggtggaa gtggtaaata cgccttttac aaacaccgct
     3961 acgactgaaa ccatcatttc actggcgaaa caactgaaaa aaacggcggt ggtctgcatt
     4021 gactcccctg gatttatcgt gaaccgggta gcccgtccat actatattga atcgttgcgt
     4081 ttgctggaag aaggccatac ggaaaaagag cagctggacc gtttgctgga agccaccgga
     4141 tttaaaatgg gtccgtttaa actgatggac ctgatcgggc atgatatcaa ttacgcagtg
     4201 agttgttccg tttgggaaca attgggccgg ccggaacgat tgaaaccatc tttattacag
     4261 gaagaaatag taaaacaggg aaagctgggc aaaaaaacgg gagaaggctt ttacaaatac
     4321 gaataaacag aaacggtaca aggattatct tcgctcctca ttcacctgat aaaccatcca
     4381 tttgtcaaaa aaaatactgg tcaccggcgg cacgggattt cttggttctt atatcatcaa
     4441 agaactggtg gagaagaatt atactgtccg cgccatccgg cggggcaata aattacccgg
     4501 ctggattccc gcatccgtgt tgcagcaagt ggaatgggtg gaaggcgata tcctggatgt
     4561 ggtttccctc gaggatgcca tggaagggat cgataccgtg attcattccg ccgcggtggt
     4621 ttcttttgta aaaaaagacc ggaaccagat gtaccaggta aatgtggagg ggacggccaa
     4681 tgtggtgaat attgcccttg aaaaaaatat tcaaaaactg gtccatatca gttcggtagc
     4741 ggcactcgga cggaatgcca tgggcgggca cgtgaatgaa gaaaagaaat gggaagacaa
     4801 taagatcaat acccattatg ccaaaagcaa attcaaggca gagctgcatg tgtggagagg
     4861 aatcagcgag ggactggatg cggtgatcct caatccgggt accatactcg gttatggtga
     4921 ctggcaccag agcagttgcg ccattttcaa acaggtttac gagggtttta aatggtatac
     4981 atcgggcatc aacggatttg tggatgtgca ggatgtgtcc cgggccacgg
//
LOCUS       DAOPMW010000240         5008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19495_length_5008_cov_7.514234, whole genome shotgun sequence.
ACCESSION   DAOPMW010000240 DAOPMW010000000
VERSION     DAOPMW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_5008_cov_7.514234"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="infC"
                     /locus_tag="PLU11_10980"
     CDS             <1..493
                     /gene="infC"
                     /locus_tag="PLU11_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPN59691.1"
                     /translation="FIRVPQVRLVGENVEVGIYSTQEALKMAQDQQLDLVEISPTVDP
                     PVCKIIDYNKFLYDKKKKEKEMKAKSKVSEVKEIRFTPNTDDHDFDFKAKHAIEFLKD
                     GNKVKAYVQFKGRAIQFQDRGQLLLLKFAERLAEIGVLENMPKMEGRRMLAMFAPKGK
                     KKA"
     gene            652..3198
                     /locus_tag="PLU11_10985"
     CDS             652..3198
                     /locus_tag="PLU11_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPN59692.1"
                     /translation="MQFLKKQGFILLLLAGCSLTVTAQNNGAPPRADTTKKPFNGPGG
                     GPSTGPKAYKEVITSKAVSDAGMFTIHKVEDKYFFEIPDSMLNRDILVVNRLSKAGAS
                     MRVGGFAGFAGDDISRNVIQFQVGPKNKIFLRTISFAEYTKDTTSSMYSAVSRSNVQA
                     IAASFDIKAMGRDSNGVVIDMTDYISGDNDILFFSSGTKSTYRIGSFQPDKSYIESLR
                     SFPINIEIKTVKTYSRMPAPSAGGFGGSQAAPGGNLTAEINSSLVLLPKVPMQPRYYD
                     KRVGYFTTGYTDYDANPQGVESISMVKRWRLEPKPGDLEKYKRGELVEPQKPIIFYID
                     PATPKKWIPYLIQGVNDWQAAFEKAGFKNAIMGKMAPTYAEDSTWSLDDARNSAIVYK
                     PSNVANASGPSIADPRSGEIMESHINWYHNIMSLLRDWYFIQCAPSDLEARKMVFDDE
                     LMGQLIRFVSSHEVGHTLGLRHNFGSSSTVPVEKLRDKAWVEANGHTPSIMDYARFNY
                     VAQPEDKIGRSGLFPRIGDYDHWAIEWGYRLFPEYKTADAEKGKLNTWIMDKLKNPRL
                     WWGDGEANQEDSRNQTEDLGDDAMKAGMYGIKNLQRIVPNLLQWTREENKDYAGLQNM
                     YEQVTGQFQRYMVHIARNVGGQMLTPKMVEQSGPVYEMNTESKQREAVDFLNKNIFAT
                     PTWLLNNEIFNRTGITGTSVVGQVQDAVISRLLNARILGKLTDAEALLGNSTYQVTEL
                     LADLKKGIFTELTSAKPVDIYRRNLQKSFVSSLINLLAPVTTTMLSTGNIVSSGGLND
                     KADAKSIIRAYLTGLRAEIAGSAARSTDNMTKYHLQDLAERINKTLNPNK"
     gene            3415..3612
                     /gene="rpmI"
                     /locus_tag="PLU11_10990"
     CDS             3415..3612
                     /gene="rpmI"
                     /locus_tag="PLU11_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPN59693.1"
                     /translation="MPKVKTNSSAKKRFKVTGTGKIMHQKAFKRHILTKKSKKRKRNM
                     RLDGTVAKSHVDFVKRLLRLK"
     gene            3670..4014
                     /gene="rplT"
                     /locus_tag="PLU11_10995"
     CDS             3670..4014
                     /gene="rplT"
                     /locus_tag="PLU11_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPN59694.1"
                     /translation="MPRSKNAVASKARRKKILKQAKGYYGKRKNVLTVAKNVVEKGMT
                     YMFVGRKLKKREYRTLWIARINAAVREEGMTYSQFINKLQTKGIELDRKVLADLAMNN
                     PETFKSLVNSVK"
     gene            complement(4288..4695)
                     /locus_tag="PLU11_11000"
     CDS             complement(4288..4695)
                     /locus_tag="PLU11_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HPN59695.1"
                     /translation="MKSILLFALAFLLLSTAPVTSVAAIVVPVATTTEPDPAKVKTAM
                     DEFNNLSRKEKRARVKEVKKEIKAYKTAKKAGKEADTDTLLLVILAILLPPLAVYLYE
                     GEINNRFWISLILTLLGWLPGIIYALVVILGQH"
     gene            complement(4793..4948)
                     /locus_tag="PLU11_11005"
     CDS             complement(4793..4948)
                     /locus_tag="PLU11_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59696.1"
                     /translation="MEQTKKYRGLWWLVFLASTAALLFAIYAHWEWLTLILPFQTTAF
                     VKAMDIM"
BASE COUNT         1520 a         1104 c         1175 g         1209 t
ORIGIN      
        1 ttttattcgg gttccccagg tacgactggt gggcgagaat gtggaagtag gaatctacag
       61 cacacaggaa gcattaaaga tggcgcagga ccagcaactg gacctggtgg aaatttctcc
      121 caccgtagat ccgccggtct gcaaaattat tgactataat aaattccttt acgacaagaa
      181 gaagaaggaa aaagagatga aagctaaaag caaagtctcc gaagtaaagg aaatccgttt
      241 cacccccaat acggatgacc atgactttga ttttaaagca aagcatgcaa tcgaattcct
      301 gaaggatggc aataaagtga aagcctacgt acagttcaaa ggaagggcca tccagttcca
      361 ggaccgcggt cagcttttac tgctgaaatt tgcagaacgc ctggctgaaa tcggtgtact
      421 ggaaaacatg ccgaaaatgg aaggaagacg aatgctggct atgtttgccc ccaaaggcaa
      481 aaagaaggcg taaccttttt acaaaaatac ctgacccgtc ttgtcaacaa gacgggtttt
      541 ttatttgtaa tgagcaaggt tacttcctaa ataaccgtct gataattcgt cacagaaatt
      601 ccttaataaa agcgctcttt ctgtatattg agccacttta aaatgatcct tatgcaattt
      661 ttgaaaaaac aaggttttat tcttttgcta ctggccggct gttccctgac tgtaacagcc
      721 cagaataatg gggcgcctcc ccgtgcggat acgacaaaaa aaccgttcaa tggcccaggc
      781 ggcggtcctt ctaccggtcc caaagcgtac aaagaggtga ttacatcaaa agccgtttcc
      841 gatgcgggaa tgttcaccat tcacaaagtg gaggataaat atttttttga aatccctgat
      901 tccatgctca accgggatat cctggtagta aaccgactga gtaaggccgg cgccagcatg
      961 cgggtaggcg gatttgccgg atttgccgga gatgatatca gccggaatgt gattcagttt
     1021 caggttggtc cgaaaaataa aattttcctg cgtactattt cctttgctga gtatacaaaa
     1081 gataccacgt cctccatgta tagtgcggtt tcccgttcaa atgttcaggc cattgctgcc
     1141 tcctttgata ttaaagcaat ggggcgcgat tccaacgggg tggtgatcga tatgacggat
     1201 tatatcagcg gagacaatga tatcctgttt ttcagcagtg gaaccaaatc cacttaccgg
     1261 atcggttcat tccagccgga taaatcatat atagagagcc tgcgttcctt cccgattaat
     1321 attgaaatca aaaccgttaa aacctatagc cgtatgccgg ctccaagcgc tggtggattt
     1381 ggcggctcac aggcagcacc gggtggtaat ctgacggcag agatcaacag ttcccttgta
     1441 ttgctcccga aagtcccgat gcagccccgc tattatgaca aaagggttgg ctatttcact
     1501 accggatata cggattacga cgccaatccg cagggtgtgg aaagtatatc gatggtaaaa
     1561 cggtggagac tggaacccaa acccggagac ctggagaagt ataaaagagg tgagctggtt
     1621 gagccacaga aaccgatcat tttttacatt gacccggcca caccaaaaaa atggattcct
     1681 tacctgatac agggtgtgaa tgactggcag gccgcatttg aaaaagcggg tttcaaaaat
     1741 gcgatcatgg gcaaaatggc cccaacctat gcagaagaca gtacctggag cctcgatgat
     1801 gcccgcaatt ctgccattgt ttacaaacct tccaatgtag ccaatgccag cggacccagt
     1861 attgcagatc cccgcagtgg cgagatcatg gaaagtcata tcaactggta tcataatatc
     1921 atgtcactac tgcgtgactg gtactttatc cagtgtgcac cttctgacct ggaagcccgt
     1981 aaaatggtgt ttgatgatga actgatgggc cagctgatcc gtttcgtttc ttcacatgaa
     2041 gtaggacata cgctcggtct ccgtcataat ttcggatcca gttccacggt tccggttgag
     2101 aaactccggg ataaagcatg ggtggaagcc aacggacata caccgtcgat tatggactat
     2161 gcccgtttca attatgtggc tcagccggaa gataagatcg gccggagcgg actctttccc
     2221 cggatcggag actatgatca ttgggccatt gaatggggct accggttgtt tcctgaatat
     2281 aaaacggcag acgctgaaaa aggaaaactg aatacctgga tcatggacaa actgaaaaat
     2341 ccccgtctct ggtggggtga tggagaagcg aaccaggaag attcccggaa ccagactgaa
     2401 gatctgggcg acgatgccat gaaagccggt atgtatggaa ttaaaaacct gcaacgtatt
     2461 gtacccaacc tgctgcaatg gacccgggaa gagaataaag actatgccgg tctgcaaaat
     2521 atgtatgagc aggtgacagg acagttccag cggtatatgg tgcatattgc ccgtaatgtt
     2581 ggcggtcaga tgctgactcc gaaaatggta gaacaaagcg gtccggttta tgaaatgaat
     2641 accgaatcaa aacaacggga agccgtggat tttctcaata agaacatttt tgccaccccc
     2701 acctggttgc tcaataacga aattttcaac cgtacaggaa ttaccggaac aagcgtagta
     2761 ggccaggtac aggatgcagt gatcagccgc ctgctgaatg cccggatttt aggcaaactg
     2821 actgatgcgg aagccctact gggtaactca acctatcagg taactgaatt gctggccgac
     2881 ctgaaaaaag gaattttcac tgaactgaca tcggcaaagc cggttgatat ttaccgccgg
     2941 aatcttcaga aatcatttgt atcctctttg atcaacctgc ttgcaccagt taccacgacg
     3001 atgttgtcca caggtaacat cgtcagcagt ggcggactca atgacaaagc cgatgcaaag
     3061 agcattatcc gggcttacct gaccggctta cgggccgaaa tcgcaggtag tgctgccaga
     3121 agtactgata acatgactaa ataccatttg caggatctgg ctgaaagaat caataaaacg
     3181 ttgaatccca acaagtaata actaattttg catattaaaa cgggcctctt cggaggcctg
     3241 tttttatttt actgccttct tggctgtttc cctgattccc agtatctttg caacccgttg
     3301 aaaaacggta gttctcttac agtctgccag gcagacaacc attgccgaag aggttcgaaa
     3361 aaggtccccc ggtggggaat gcctcaaggg tataattatt aaatactggg taacatgcct
     3421 aaagtaaaaa caaactccag tgccaaaaaa cgtttcaaag taacgggcac cgggaagatc
     3481 atgcatcaga aagcattcaa gcgtcacatt ctgacgaaga aatcaaaaaa acgtaaacgt
     3541 aacatgcgcc tcgacggaac tgttgcaaaa tcacatgtgg attttgtaaa acgcctgctg
     3601 cgtctgaaat aaaccttacg ttcaacacat ttattcatca gtcagtctgc tgactaaaaa
     3661 ttatacacta tgcctcgttc aaaaaatgct gtggcttcca aagcccgccg caaaaaaata
     3721 ttaaaacaag ccaaaggtta ctatggcaaa cgtaaaaacg tattaacggt tgccaaaaac
     3781 gtggtggaga aagggatgac ctacatgttt gtaggccgta aactgaagaa aagagaatac
     3841 cgcactttat ggattgcccg tatcaacgcg gctgttcgtg aagaaggaat gacctattcc
     3901 cagttcatca acaaactgca aaccaaaggc atcgaactgg atcgtaaagt actggctgac
     3961 ctggccatga acaaccccga gacgttcaag agtctggtga attcagtgaa gtaattcttc
     4021 aaagtagtta tagcaaaagg cccggaagtt ccgggccttt tgcgtaagca gaccagaagg
     4081 aattacaggg tagggataga atgagagaag gatgagtaat gagtaatcag taatgagtag
     4141 cccgggaagg gaactacaga gtagaaggaa gatgagagaa ggatgagtaa tgagtaatca
     4201 gtacctaaag taatgcgccc tccgaaaatc ggagggcgta tttataatta gagtctacca
     4261 cacttactca ttactcatta ctcattccta gtgttgtccc agtatcacca ccaacgcata
     4321 aataattcca ggtaaccaac ccaacaacgt cagaatcagg ctgatccaaa aacgattatt
     4381 tatctctcct tcgtagagat atacagccag cggggggaga aggatcgcca ggatcaccag
     4441 caggagggtg tccgtgtcgg cttctttacc agcttttttg gctgttttat aggctttaat
     4501 ttccttcttc acttccttca cacgggcccg tttttctttc cgggagaggt tgttaaactc
     4561 atccattgcc gtttttactt tggcaggatc aggttcagtt gttgtggcaa caggaacgac
     4621 tatagccgct acagatgtga cgggagcggt gcttaagagc agaaaagcca gggcaaaaag
     4681 taaaatagat ttcatacact acaattttag gtttatgtca cgaaaaatat gccggattca
     4741 caaaaataat cccgcgcatg gcgggactat catatatagt tataattaat atttacatga
     4801 tatccatcgc ctttacaaag gccgtagtct gaaagggcag gatcagggtc agccattccc
     4861 aatgggcgta aattgcgaaa agaagggcgg ctgtcgaagc caggaaaaca agccaccaaa
     4921 ggcctctata cttttttgtc tgctccatgt tgaaaaattt taggcaaaaa taaggtccgg
     4981 acggtaaacc aaaaaacaga tttttaaa
//
LOCUS       DAOPMW010000241         5006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19506_length_5006_cov_6.429812, whole genome shotgun sequence.
ACCESSION   DAOPMW010000241 DAOPMW010000000
VERSION     DAOPMW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19506_length_5006_cov_6.429812"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..707
                     /locus_tag="PLU11_11010"
     CDS             63..707
                     /locus_tag="PLU11_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HPN59697.1"
                     /translation="MALDILQEGNDRFIKNLKAQRDLLGQVNATREGQWPFATILSCM
                     DSRTSAELIFDQGLGDIFSLRIAGNIVNTDILGSMEFACKVAGSKLIVILGHTKCGAV
                     KGACDHVEMGNLTELLSKLQPAVYQERETVNQEMRNSKNPVFVENVSSINVRRTVKSI
                     IERSYIIEQMVETGAIGVVGAMYNIDTGRVEFYDDVQYIKDDLNPEFSVADLRH"
     gene            738..3263
                     /locus_tag="PLU11_11015"
     CDS             738..3263
                     /locus_tag="PLU11_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HPN59698.1"
                     /translation="MQQPFDADEVIHQLKHYLPAQQALKDFIHHNSLHAFQHQKFYDG
                     IFKASRIFGFQVHLQLGEYREMYQTGRIREDVVDRVIEWRKGKKEMISWKEKMLLQGY
                     NPVFEPRIGKLRARWKQFYGIDLDNRVHPLLFRILCSFLDQGIALKELPVVNRSFTTT
                     IRELESKSLVSFFNTRKVRQLFISGDYTIDGLLKQVAGDEAYFEQYLFDQQFAHHGWS
                     GFVSAAEDNPQTLPDRKQITLRELVEFELLLELDALYKELGNTWKPLTTHIAEAPADL
                     FAPVPQTEFTEVIACWQTAFEWSYYDSVLRGMLMAKPKPVPEQKSLQAIFCIDEREDS
                     IRRHIEAVDKQAETFGAPGFFGVEFYFQQQGSKFYDKLCPAPVTPKFLIKESEASTVH
                     QNDLIYTKYTHGIVSGFFLSIGFGFWALAKKLQLLFRPKMSPAISNAYGHMDKKSRLS
                     IENQDPAHIENGLQIGYTVEEMTARAEAFLRGIGLIKDFAPLVYIVAHGSSSANNPHH
                     GAHDCGACSGRPGATNARVQAFILNHEKVRNILAERGISIPASTQFVGCMHDTAADVM
                     DYYDEDVLDETNGQAHLLNIQRFETALNLNAKERSRRFASINTKQELEQVRKAIHNRS
                     VSLFEPRPELGHGTNTLAIIGRRQVSKGLFLDRRAFLNSYDYTTDPDGSILSAVMRPI
                     GLVCGGINLEYYFSRVDNIKLGAGTKLPHNVMGLFGVANSSDGDLRPGLPWQMIEVHD
                     PVRLLVIVEQSPEIVLKAIQSAPAVFEWYQNEWVHIVALHPATQQFYYFRNGVFEPYQ
                     LLTAADEIRTIHNMEEFIEGAREMETNHIVHATEENLPVYLLD"
     gene            3323..>5006
                     /locus_tag="PLU11_11020"
     CDS             3323..>5006
                     /locus_tag="PLU11_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN59699.1"
                     /translation="MGQLLVLAVLVPLLAFFATLLWQNKNERPIAWIVRIAKVVNIGL
                     AVLFAAWWILDGLKAVDYQLLTLYKSDHFVFALHFYYDEITAVFSLTGAVLFFLVATF
                     SKYYMHRDQGFKRFFNTILLFSTAYNLIILSGNFETLFIGWEIKGICSFLLIAFYRDR
                     YLPAKNAFKAVSNYRISDVSLMLAMWMMHHLMNQNISFPQLGLAKETALATASPFMSV
                     FIVCMMILPAVVKSAQFPFTTWLPRALEGPTSSSAIFYGSLSVHIGVFLLLRTHPFWE
                     DMLWAKILIIVIGALTAITATVIARVQPTVKTQIAYSSAAQIGLMFIEIALGFHWLVL
                     LHFAGNAFLRTYQLLVSPSVLNYLVHHQIFHYTAPVHKAVSRWKATLYVLGIKEWNLD
                     TLLYRYGWKPFKTLGAVFGFLQNKVFANTLAIMGVFLLIAGYNKPDSFQSQVSGMPML
                     LMSVALVLFLYSFSSRGRAITAWWYLLTGHIFILAAVLFNAPHIYFSELFFYASGVLI
                     AFLLGYYCLIKIERIDQDISLSRFHGYVYEQENTGFLFLLAAMGMLGFPITAA"
BASE COUNT         1268 a         1143 c         1244 g         1351 t
ORIGIN      
        1 aaaaaagaaa ttataaaatg agagtacata ctaaagaaac acaatcacac ctgactccgc
       61 ggatggccct tgatatcctg caggaaggaa atgaccggtt tatcaaaaac ctgaaagcac
      121 agcgcgatct gctcggccag gtaaatgcca cccgcgaagg acagtggccc tttgccacga
      181 ttctgagttg tatggacagc cgtacatctg ccgaactgat ttttgaccag ggactggggg
      241 atattttttc gttgcgcatt gcgggaaata ttgtgaatac ggatatcctc ggcagtatgg
      301 aatttgcctg taaagtagcc ggttccaaac tgatcgtgat tttgggccat acaaaatgcg
      361 gtgcggtaaa aggagcctgt gatcatgtag aaatgggcaa cctgaccgag ctgttgtcaa
      421 aactgcaacc ggctgtttac caggaaaggg aaaccgtaaa tcaggaaatg cgtaactcaa
      481 agaatcctgt atttgtagaa aatgtttcct ccatcaatgt tcgccgtacg gtgaagagta
      541 ttattgaacg gagctatatc attgaacaaa tggtggaaac cggagcgatc ggggtggtag
      601 gtgctatgta taatattgat actggccggg tagagttcta tgatgatgta caatacataa
      661 aagacgatct gaatccggaa ttcagcgtgg ctgatttgcg gcattaagca gggatcccgg
      721 gtttataaat aaaatttatg cagcaaccat ttgacgcgga tgaagtgatt catcagctga
      781 aacactatct ccccgcccag caggcattga aggattttat ccatcacaac tcgttgcatg
      841 ctttccagca ccagaaattc tatgatggca ttttcaaggc ctcccggatt tttggattcc
      901 aggtacacct gcaattagga gaataccggg agatgtacca gaccggacgt atccgggagg
      961 atgtggtgga ccgggtgatt gaatggagaa aagggaaaaa ggaaatgatc tcctggaaag
     1021 agaaaatgct gttgcaggga tacaacccgg tttttgaacc tcgcatagga aaattgcggg
     1081 cgaggtggaa acagttttac ggcatcgatc tggataaccg ggtgcacccc ctgttattcc
     1141 gcattctctg cagcttcctt gatcagggta ttgccctgaa ggaactgccc gtggtcaacc
     1201 ggagtttcac cactaccatc cgtgagctgg agtcaaaatc attggttagt ttttttaata
     1261 cccggaaagt caggcaactt tttatctccg gggattatac catagacgga ttgctgaagc
     1321 aggtggcagg ggatgaagcc tactttgaac aatacctttt tgaccagcaa ttcgcccatc
     1381 atggctggag tggttttgta agtgcagcag aagacaaccc gcagacattg ccggatcgta
     1441 aacagataac acttcgtgaa ctggtcgagt ttgaattgct actggaactg gatgcattgt
     1501 acaaggaact gggcaacacc tggaaacccc tgaccactca tattgctgaa gctccggctg
     1561 atttgtttgc gcctgtacca caaacggagt ttaccgaagt gatagcctgc tggcagaccg
     1621 catttgagtg gagctattat gacagcgtac tgcgggggat gctgatggca aaaccgaagc
     1681 cagttccgga acagaaaagc ctccaggcca ttttttgtat tgatgaacgg gaagattcca
     1741 tccgtcgtca tatagaagcc gtggataaac aggcagaaac attcggcgca cctggttttt
     1801 ttggggttga attttatttt cagcagcagg gcagtaagtt ttacgataag ttatgtccgg
     1861 cccctgtaac acctaaattc ctgatcaagg aatcggaagc cagcaccgta caccaaaatg
     1921 acctgatcta tacaaaatat acgcacggca ttgtttccgg atttttcctg agtattggtt
     1981 tcggattctg ggcactggca aaaaaactgc agctgctttt ccgtcccaaa atgagcccgg
     2041 ctatttccaa tgcctatgga catatggata aaaaatccag gctgtccatc gaaaaccagg
     2101 atccggcgca tatcgaaaat gggttacaga tcggatatac cgtggaagag atgacagccc
     2161 gggcggaagc gttcttgcgt ggaattgggc tgataaaaga ttttgccccc ctggtctata
     2221 tcgtagccca tggaagcagc agtgccaata acccgcatca cggggcccat gattgtggcg
     2281 cctgcagcgg aagacctggt gccaccaatg cacgggtaca ggcctttatt ctgaatcatg
     2341 aaaaagtcag aaatatattg gcagagcggg gtatctctat tccggcaagc acccagttcg
     2401 tcggttgtat gcacgatacc gctgcggatg taatggatta ttatgatgag gatgtgctgg
     2461 atgaaactaa cggacaggca cacctgctga atatccagcg ttttgaaaca gccctgaacc
     2521 tgaatgccaa agagcgaagc cgtcgttttg cctccatcaa taccaaacag gaactggaac
     2581 aggtacggaa agccattcat aaccggtcag tttctttatt tgaaccaaga ccagagctgg
     2641 gtcatggcac gaatacctta gccattatcg gccgtcgcca ggtcagtaag ggattgttcc
     2701 tggacagacg ggccttcctc aacagttatg actatacgac cgatcccgat ggttcgatcc
     2761 tgtctgctgt tatgcggccg atcggactgg tgtgtggcgg gatcaacctt gagtattatt
     2821 tttcccgggt ggataatatc aaactcgggg caggtaccaa attgccccat aatgtgatgg
     2881 gtttattcgg ggtagccaac agcagtgacg gcgatctgcg gcccggtctt ccctggcaga
     2941 tgattgaagt acatgacccg gtacgtttgc tggtgattgt agaacagtct ccggaaatag
     3001 tattgaaggc gattcaatct gcgcctgccg tatttgagtg gtaccagaat gaatgggtgc
     3061 atatagtggc ccttcacccg gcaacgcagc aattttatta tttccgcaac ggcgtatttg
     3121 aaccttacca gttgctgacg gctgcagacg agatcaggac cattcacaat atggaagagt
     3181 ttattgaagg ggccagggag atggagacca atcatattgt acatgccacg gaggaaaacc
     3241 tgccggtgta tctgctggat taaaaggatc gggttattaa tacaggcaga tgccacaagg
     3301 gcctctgcaa aaaatgaatt caatgggtca attacttgta ttagctgtac tggtgccgct
     3361 gctggctttt tttgccaccc tgctctggca aaataaaaat gagcggccca tcgcctggat
     3421 cgttcgtata gcgaaagtgg tcaatatcgg gctggccgta ttatttgccg cctggtggat
     3481 cctggatggc ctaaaagcgg tggattatca gttactcacg ttgtataagt ctgatcattt
     3541 cgtttttgcc cttcattttt attacgacga aataacagcg gttttcagcc tcaccggtgc
     3601 ggttctcttt ttcctggtgg ccacattcag caagtattat atgcaccgtg atcagggctt
     3661 caaacggttt tttaacacaa ttctcctttt ttcaacggct tataacctga ttatcctatc
     3721 cggcaatttt gaaacgcttt ttatcggctg ggagatcaag ggtatctgtt ctttcctgct
     3781 catcgcgttc taccgtgacc gttacctgcc ggcaaaaaat gcctttaaag ccgtgtccaa
     3841 ttaccggatc agtgacgtct ctctcatgct ggccatgtgg atgatgcatc acctgatgaa
     3901 ccagaatatc agtttcccgc aactcggact tgccaaagaa acggcactgg ccaccgcttc
     3961 gccctttatg tccgtgttca ttgtttgtat gatgatcctg ccagctgtag tgaaatcagc
     4021 ccagtttccg tttaccacct ggctgcccag agcactggaa ggtcccactt catcatcggc
     4081 gatcttttac ggttcattga gtgtgcatat tggtgtattc cttttattac gtacacatcc
     4141 cttctgggaa gatatgcttt gggccaagat cctgatcatc gttatcggag cgctcacggc
     4201 cattaccgct acggtgattg cccgggtaca acctacggtg aagacacaga tcgcttattc
     4261 atcggcggca cagatcggac tgatgtttat tgaaattgcc ctaggtttcc attggctggt
     4321 tctgcttcac tttgccggca atgcttttct ccgcacctac cagctgctgg tgtctccatc
     4381 cgtactcaac tatctcgtac atcaccagat ttttcattat accgcaccgg ttcacaaagc
     4441 agtttcccgc tggaaagcta ccctgtatgt cctggggatt aaagaatgga accttgacac
     4501 attattatac cgctatggat ggaaaccctt caagacgctg ggtgctgtat ttggtttttt
     4561 gcaaaacaag gtttttgcca ataccctggc catcatgggt gtctttctgc tgattgcggg
     4621 gtataataaa ccggattcct tccagagcca ggtcagcggg atgcccatgc tgctcatgtc
     4681 ggtggcactt gtattgtttc tctattcttt ttcatccagg ggcagggcta ttacagcctg
     4741 gtggtacctg ctgacaggac atatttttat actcgccgct gttttattca atgcaccgca
     4801 tatttatttc agtgagttgt ttttctatgc cagcggggtg ctgattgcct ttttactggg
     4861 ttattattgc ctgattaaaa tagaacgtat cgatcaggat atttctttgt cccgtttcca
     4921 cggttatgta tatgaacagg agaataccgg gttcctcttt ctgttggcgg ccatgggcat
     4981 gctgggtttc ccgatcacgg ctgcgt
//
LOCUS       DAOPMW010000242         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19550_length_4996_cov_10.778385, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000242 DAOPMW010000000
VERSION     DAOPMW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19550_length_4996_cov_10.778385"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /gene="rlmD"
                     /locus_tag="PLU11_11025"
     CDS             complement(<1..1161)
                     /gene="rlmD"
                     /locus_tag="PLU11_11025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPN59700.1"
                     /translation="MRKKKKNIVLQNVRVDDYAAEGKSLARVDGKVIFMEGVVPGDLV
                     DVRLLKNKKDWAEGMVLKIHEFSGERVKPFCSHFGVCGGCQWQMLPYEKQLQYKSKQV
                     SDVLQRIGKVPLPPIQPILGAAETRYYRNKIEYTFGNKRFLTKEEIGDETINGQQDVA
                     GFHAKGFFDKVVDIDTCYLQDEPTNAIRLFVKQLAITKGWPFYDIRNHTGFLRTMQVR
                     LCQTGELMVNIMVGEDDQDKIRILMEAVKNEFPAITTLLYTINLKWNDSLYDLEPVTY
                     HGKGYVIEKLENFQFKIGPKSFFQTNTKQGEELYKVTREFAELSGTETVYDLYCGTGS
                     IGIFVSEKAKKIIGVEMVEAAIEDAKENAALNNLHSTAFFAGDVIDICNDDFF"
     gene            1316..2749
                     /locus_tag="PLU11_11030"
     CDS             1316..2749
                     /locus_tag="PLU11_11030"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HPN59701.1"
                     /translation="MKKALIFTGLLWSLAAAAQTGYEINVTFKPFKNQYIYLGHYFGK
                     SYPIIDSAMLDENSQASFKGTKALSGGIYLVGYPNKAGFFEILIDKQQHFSVVADTAT
                     IAKGASYVNSPDNVLFTNYQQRMSAFGRAISGLQAQLKVAANKADSAKITEALTAEDK
                     QVGNYREELINNNPDALLTTLLICMREPELKGALKNPANKADSTEAYNYFKSHFWDGV
                     NFWDGRLAFTPFFEEKLEKYFSQLVLPHPDTVIKEIDRMLSYASINEEMNRFLLVKFI
                     NRYLNQKYMWEDAVFVHLFEKYFSQKTYSWLNEAGKKTITERAYSLMANILGTPASDI
                     ELNNTENKPASLYSTSASFTIVAFWDPTCGHCKEVLPKLDSFYRSKWKADGLKIFAVA
                     KETEGTKADWLKFVKDNKLTAWTHVYYSKEADKARTDNGIPGYSQLYDVQSFPTLYLL
                     DKDKRIIAKKLTYQQLDEIIQLKKKGQ"
     gene            2770..4716
                     /locus_tag="PLU11_11035"
     CDS             2770..4716
                     /locus_tag="PLU11_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN59702.1"
                     /translation="MNRKNLLLLLLCGSMLQASTQTLFTYGPYKADAKDFLRAFNKNN
                     MNVAGNKSLAMKEYLDLYIKSKLKVREAYERRYDTLITIRTEVENLRAQIADGFMTDP
                     EIINRLQKEAFQRSLKNIRVAHIFIAFRNSMGVTDTLVAQKKKEQLMARLQKGEEFGK
                     LAKEFSDDPSVKTNNGEIGFITVFTLPYDFENAIYATAPGKVTVPVRSKIGYHIFKNL
                     EEKKSPGKMKARQILLAFPPDATVQVKQQLARLADSLHKRLLAGDAFATLATSFSNDY
                     ISAANGGLMPDISVGQYDALFEKNLWALSKDGAISKPFQTSHGWHILKRESVKPVITD
                     ANNKDNLQELQQKIIADSRWKASKDHIYKQVRDKAGVKLLLNNEAGFRAYSDSILDRL
                     PMQEAGRLFTPSTPVFTIGTEVYDLAAWFNYASVYRFRTDGSGAKAHELVKEEWLQHA
                     MFEYYKKHLEEFNEEFRSQMAEFRDGNLFFEIMQQEVWTKAQSDTVALQALYEKNKKQ
                     YTWQQSADVVIFFCSDPAVAQLIHDKVKANPAGWKNITDQYSEKVLADSSRYEWSQIP
                     NLNKQVPKAGMMTAPVINQTDNTASFAYIFNAYPQLLQRSYSEAKGIVINDYQAILEK
                     EWEEVLKKKYPVKVEEKVLAEIAK"
BASE COUNT         1383 a         1169 c         1160 g         1284 t
ORIGIN      
        1 aaaaaaatcg tcattacaga tatcgatcac atcgccggca aaaaaggcgg tgctgtgtaa
       61 attattcagg gccgcatttt cctttgcgtc ttcgatggcg gcttccacca tttccacccc
      121 gatgatcttt ttcgcttttt cactgacaaa gatcccgata ctgcccgtac cacagtataa
      181 gtcgtacacg gtttctgtac cgctgagttc cgcaaattca cgggtcactt tatacaattc
      241 ttctccctgc tttgtattgg tctggaaaaa ggacttggga ccgatcttaa actgaaaatt
      301 ttccagtttt tcgatcacat atcctttccc gtggtacgtc actggttcca ggtcgtacag
      361 actgtcattc catttcagat tgatcgtata taacaaagta gtaatggcgg gaaattcgtt
      421 tttcaccgct tccatcagga tacggatctt gtcctggtca tcttcgccca ccatgatatt
      481 caccatgagt tcgccggtct ggcaaagccg tacctgcatg gtccgcagga agccggtatg
      541 gttgcggata tcataaaaag gccagccctt ggttatcgcc agttgtttta cgaaaagccg
      601 gatagcattc gtgggctcgt cctgcaggta acaggtatca atgtccacca ctttatcaaa
      661 aaagcctttg gcatgaaagc cggccacatc ctgctgtccg ttgatcgttt catccccgat
      721 ttcttctttg gtcagaaagc gcttattgcc gaatgtgtat tcgatcttgt tccggtagta
      781 ccttgtttca gccgcaccca ggatgggttg tatcggtggc aggggcactt tcccgatccg
      841 ctgaagcaca tcggaaacct gcttgctttt atactgcagt tgtttttcat aaggcagcat
      901 ctgccattga caaccaccac ataccccgaa atgggagcag aagggcttga cccgttcgcc
      961 ggagaactca tggatcttca gcaccatgcc ttcagcccag tcctttttgt tcttcaggag
     1021 gcgcacatcc accaggtcac ctggcaccac gccttccata aaaatcactt tcccgtccac
     1081 ccgggccagg ctttttcctt cggcggcata gtcatccacc cggacatttt gcaatacgat
     1141 atttttcttc ttttttctca cgccgcaaat atacctaagc ggccccgccc tgccgccgga
     1201 attttccttt ccccaggcaa cgaattgtcc gtctttatcc tcttaatcaa cgattttcgc
     1261 agggtctctt tcgggggctg cccaatttta atatctttac cggattcaac agattatgaa
     1321 aaaagcattg atttttacgg gactcctttg gtcactggca gctgcggcgc aaaccggtta
     1381 tgagatcaac gtgaccttta agccctttaa aaaccagtat atctacctgg gtcattattt
     1441 tggtaaaagc tatcccatta tcgattcggc tatgctggat gagaacagcc aggccagttt
     1501 taaaggcaca aaggccttat cgggaggcat ctatctggtt ggctatccca ataaggccgg
     1561 tttttttgaa atcctgatcg ataagcaaca gcatttttca gtggtggccg ataccgctac
     1621 tatcgctaaa ggggccagtt atgttaattc ccccgataat gtacttttca cgaactatca
     1681 gcaacggatg tcggcttttg ggagagcgat cagtggcctg caggcacagc tgaaagtggc
     1741 tgctaataaa gcggattctg cgaaaatcac cgaagcactt acggcagaag acaaacaggt
     1801 aggcaactat cgggaggaac tgatcaacaa caatccggat gccctgctga ccaccttgct
     1861 gatctgtatg cgtgaacccg aactgaaagg cgcgttgaag aatccggcaa acaaagcgga
     1921 ttccacggaa gcttataatt atttcaaatc ccatttctgg gatggcgtga atttctggga
     1981 cggacgactg gcctttacgc cgttttttga ggaaaaactg gagaaatact ttagccagct
     2041 ggtactgccg catccggata cggtgatcaa agaaattgac cggatgctca gctatgccag
     2101 tatcaacgag gaaatgaacc gttttctcct ggtgaagttt attaaccgtt acctcaatca
     2161 gaaatacatg tgggaggatg cggtttttgt acaccttttt gaaaaatatt tctctcagaa
     2221 aacctattcc tggctcaatg aagcgggtaa gaaaacaatc acagaaagag cctatagcct
     2281 gatggcgaat atcctcggta caccggcatc ggatattgaa ctgaataata cggaaaacaa
     2341 gccggcatca ctttatagca caagcgccag ttttacgata gtggcgttct gggatcccac
     2401 ctgcggacat tgcaaggaag tgctgccgaa actggattcc ttttaccgca gcaaatggaa
     2461 ggccgacgga ttgaagatct ttgcagttgc caaggaaacc gaaggcacca aggctgactg
     2521 gctgaagttt gtaaaagaca ataagctcac cgcctggact catgtttact attcaaaaga
     2581 ggcggataaa gccagaacgg acaatgggat acccgggtat tctcagttat atgatgtgca
     2641 gtcattccct actttatatt tgctggacaa agacaaacga attatcgcca aaaaactgac
     2701 ctatcagcag ctggatgaaa ttatccagtt gaaaaagaaa gggcagtagt gtttaaaatc
     2761 agatccggta tgaacagaaa aaaccttctc ctgctgttgt tatgcggcag tatgcttcag
     2821 gcatccacgc aaaccctatt tacttacgga ccgtacaaag cggatgcaaa agatttctta
     2881 agagccttca ataagaataa catgaatgtc gcgggcaaca agtccctggc gatgaaggag
     2941 tacctcgatt tatatatcaa atcaaaatta aaagtcaggg aggcttatga aagaaggtat
     3001 gataccctga tcacgatccg caccgaggtg gaaaacctgc gggcccagat tgcggatgga
     3061 ttcatgaccg atccggaaat catcaaccgg ttgcagaaag aagcctttca acgcagcctg
     3121 aaaaatatac gcgtcgctca tatttttatt gcgttccgga atagtatggg tgttacggat
     3181 accttggtgg cccagaaaaa gaaggagcag ttgatggccc ggcttcagaa aggggaggag
     3241 tttggtaaac ttgcaaaaga gttttcggat gatccctctg tcaaaacaaa taacggggag
     3301 atcggtttta ttactgtttt caccctgcct tatgattttg aaaatgccat ctacgccaca
     3361 gctcctggta aagtaaccgt accggtccgc tcaaaaatcg ggtaccatat ttttaaaaac
     3421 ctggaagaaa aaaaatcgcc gggaaaaatg aaagcccgcc agatcctgct ggcatttccg
     3481 ccggatgcta ctgttcaggt aaaacaacaa ttggcccgcc tggctgattc tctacacaaa
     3541 cgcctgctgg ccggtgatgc tttcgccacg ctggccacca gtttcagcaa tgattatatc
     3601 agtgccgcga atggagggtt gatgcccgat atatccgttg ggcagtatga tgcgttgttt
     3661 gaaaaaaatc tttgggccct gtcaaaagat ggtgcgatca gcaagccctt tcagaccagt
     3721 catggctggc atatcctgaa gcgggaatcc gttaagcctg ttattactga tgccaataat
     3781 aaagacaacc tgcaggaatt acagcaaaag atcattgcag acagtcgctg gaaagcatca
     3841 aaggatcata tttacaaaca ggtcagggat aaagccggcg ttaaactgct gctgaataat
     3901 gaagcgggat tccgggccta ttccgatagt atactggatc ggctgcccat gcaggaagcc
     3961 ggccgtttgt tcactccttc caccccggta ttcacgatcg gtactgaagt gtatgatctg
     4021 gctgcctggt tcaattatgc cagtgtgtac cggttccgta ccgacgggtc aggtgccaaa
     4081 gcccatgagc tggtgaaaga agaatggctg cagcatgcca tgttcgaata ttataaaaag
     4141 cacctggaag aattcaatga ggaattccgc agccagatgg ctgagttcag ggatggtaat
     4201 ctctttttcg aaatcatgca acaggaagtc tggaccaaag cacaaagtga cactgtcgct
     4261 ctccaggctt tatatgaaaa gaataaaaaa caatatacct ggcagcagag tgcggatgtg
     4321 gtgattttct tttgttcaga tcctgcggtg gctcagctca tccatgataa agtaaaagcc
     4381 aatccggccg gctggaagaa tatcaccgat cagtattccg agaaagtgct ggctgattcg
     4441 tcgcgttatg aatggagcca gattcctaat ctgaataagc aggtacctaa agccggtatg
     4501 atgacggcac cagtaatcaa tcaaacagat aataccgcct cctttgccta tatctttaat
     4561 gcttacccgc aattgctgca acgcagttat agtgaggcaa aaggaattgt gatcaacgat
     4621 taccaggcga tcctggagaa agagtgggag gaagtgctga agaagaagta cccggtgaag
     4681 gtggaggaga aggtgttggc ggagattgct aaataaggta cagccctgcg gggcggcaaa
     4741 gagttcaaaa gagaatacaa ggaggaaaaa gaggtcgtga taagggaaaa gtataataaa
     4801 aaagctccag ccagttggtt gaagcttttt ttatttcatt tactattgtt ctgaatggtc
     4861 agtaatcagc gactatctat tagaaaaacc cttccattat cacgccctct tttttctctt
     4921 ttaaaaaact cttttgacct ctttagcgcc gcccgcaggg cctttatatc tccttcacca
     4981 ctcccgccac cagcaa
//
LOCUS       DAOPMW010000243         4987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19603_length_4987_cov_5.084347, whole genome shotgun sequence.
ACCESSION   DAOPMW010000243 DAOPMW010000000
VERSION     DAOPMW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19603_length_4987_cov_5.084347"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PLU11_11040"
     CDS             complement(<1..1498)
                     /locus_tag="PLU11_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN59703.1"
                     /translation="MTKKNCLQFRIHFPELVTCSGIRRTVSKNLTVLLLALALPFFVL
                     AQERTITGHVHDAETGDPVVGATVSIKGKSSGVITDALGAFKLKAADADVIEISSVGY
                     QTQEMQAGSVADLQIKLKVLNKQLNEVVVVGYGTKKRSDVTGSVATVPKNRLEKLPVT
                     NVLHAIEGSVAGVNITQFSSVPGSSANVQVRGQNSLNGGTGPFIVVDGVPFSKTGSVT
                     NDINPNDIASIEILKDASATAIYGVNGANGVILITTKRGATGKPVIRYNGYVGFDNIS
                     NTMTPQSPAEYVQKYADWWKQTYPTTTQANVLPNAYEIANYNAGKTTDWLKEVTQQGI
                     MQDHNLSISGGNKEVKYYISGDYLKQKGAVQGYSYSRANLRANLDVNVTDFLTLGTSL
                     FFNNNNYGGGRSNFYLAAAMSPYGTLRNSSGAYEIYPMNPELLYKNPMLGLYTDREDK
                     SSNLNGNVFAEVKFSGLLKGFKYRLNTGYTYFTTRYGYYEGRNTGNTIG"
     gene            complement(1714..>4987)
                     /locus_tag="PLU11_11045"
     CDS             complement(1714..>4987)
                     /locus_tag="PLU11_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN59704.1"
                     /translation="FNQLWIGTDRGGITLVDRATKQFRYIESGDGSDKLSSETVSSIL
                     RDKESRIWIGTIKGGCNIIDVQKGRFTSISRQPFSNNTLPVSTVYSFCEDRDRDLLIG
                     TDGGGLSVWNRVTNQFTNYNHQPGDKGSLSHNSVTRILQDNTGIIWVATYGGGISHFN
                     KQKGRFEPVPCIDERTGYENKNISLLYEDRQHNIWASAFNDGKLYLFDRRLNKFELFD
                     PGLTNLISMLEDRKGNLWVGNSSLLVKLDLLQKKHTSFSIGRPVRSIFEDKAGRFWLG
                     TEGGGLLLFDREKGQLIERFSDADGLCNNGVLNILEDSTGHLWMSTFNGLSQFDFASR
                     VFKNYYQSDGLQSNQFSYRAATQLRNGELAFGGINGFSVFKPEQIVPRAYMPPVYITN
                     ILVNGKPVAELSRFIRPIRNNDIAKMVVPFDQAVFNFSFTALEYTSPEKIKYGYFLEG
                     WDKNWNYSGNINNINYNNLAEGSYTLHLKSTNVNGQWNEQETLLKLVILPPWYRSWWA
                     YLIYMMAAGSLIWLYLRYRSQQAHLKYKMKLTELNAEKEKEINEKRQSFFTNITHEFR
                     TPLTLIINPLKDLVNGKPKTESADIELVYRNARRLLSLVDQLLLFRKTESETGKLHVA
                     RLDTRQMGEETFSFFIQQARSKGIRYTYECPDQLPAVYGDREKLQIVFYNLLSNALKY
                     TPADGQVSFRIWEEGDKVLAEVADSGPGIPPETGSRLFEKFYQVNKKEAKAKAGFGIG
                     LYLVKQLVEEHKGQIGYESTPGKGTRFFMALKTGYQHFGDVPVGESSGESEHELLDEI
                     IAGEEPVPVLKWQTDGLESVVSEKHSILITDDNPQMRSYLAQVFKEDFIVYEAGSGEE
                     AIKMAAELMPDIIISDVVMENGSGIEFCKMVKDTAALSHIPFVLITGSFSQESKLKGI
                     ESGADDYITKPFEKDMLVARVQSLIKKQQNLQKYFYNEITHQQNTLNISGEYKEFLES
                     CIAAVERNLDKDDFNIQVLAQDMGMSHSKLYKKVKTISGQSANAFIRYIRLRKAAELF
                     INSDYNINETAFYVGIKDIKYFREQFTRTFGMKPSEYIEKYRKIHGRNFKLNDKLTRE
                     GE"
BASE COUNT         1293 a         1209 c         1047 g         1438 t
ORIGIN      
        1 aaccaatggt attgccggta ttccttcctt catagtatcc atagcgggtg gtgaaatacg
       61 tatagccggt attgagacgg tatttaaatc ctttgagtaa gccgctgaat tttacttcag
      121 caaatacatt cccgttcagg ttactgctct tgtcttcacg atcagtatat aatccgagca
      181 tcggattttt gtaaagcaat tcgggattca tcgggtaaat ctcataagca ccggaactat
      241 tccggagtgt tccataagga ctcatggcag cagccaggta aaagttggaa cgtccacccc
      301 cgtaattgtt attattaaag aacagggacg tacccaaggt caggaagtcg gtcacattta
      361 catccaggtt ggcacgcagg ttggcacggc tgtatgaata gccctgcacc gctccttttt
      421 gtttaagata atctccggag atatagtatt tcacttcttt attaccacca gagatactca
      481 ggttatgatc ctgcatgatt ccctgttggg ttacttcttt cagccagtca gttgttttac
      541 cggcattata attggcaatc tcgtaagcat tcggtaatac attagcctgg gtggtggtgg
      601 gataggtttg tttccaccag tcggcatatt tctgtacata ttcagccgga ctctgtggtg
      661 tcattgtatt agagatatta tcaaatccta catagccatt gtaacgaatc accggtttac
      721 cggtagcccc tcttttagtg gtgatgagaa tcacgccatt ggccccattc accccgtaga
      781 tcgcggttgc tgaggcatct ttcaggattt caatggaggc aatatcattg gggttaatat
      841 cattggtcac actgcccgtt ttgctgaatg gcacaccatc cactacaata aacggacctg
      901 taccaccatt caacgaattc tgaccacgaa cctggacgtt ggcagaacta ccaggcacag
      961 acgaaaactg ggtgatattt acacctgcaa cagaaccttc aatagcatgc agcacattgg
     1021 ttacaggcag tttttcaaga cgatttttgg gaacagtggc caccgaaccg gttacatcgg
     1081 accttttctt ggttccataa cccacgacca ctacttcatt cagctgtttg tttaacactt
     1141 tcagctttat ctgcagatcg gcaacacttc ccgcctgcat ttcctgggtc tgataaccca
     1201 cactggaaat ttcgattaca tctgcatcgg ccgcttttag tttgaaagca cccagggcat
     1261 cggtgattac ccctgaactt ttgcccttaa tggacactgt ggcaccaacc accgggtcac
     1321 cggtttcggc atcatgcacg tgaccggtaa tggttctttc ctgggctaat acgaaaaatg
     1381 gaagtgcgag tgcgaggagc aggactgtca ggttttttga aacagtccgc ctgattccgg
     1441 agcatgttac caattccggg aaatgaatcc ggaactgtaa gcagttcttt ttagtcatat
     1501 gacggcaatt ttagtgagtg aatgaagaag gtctggttgg caatccttct cttagagcat
     1561 ccaaatatga gctcgttttt tgccggcagc gggggaataa ttagtacaaa aagggggtaa
     1621 attcggattt tttcaaaaaa acaggggggt aaaacaggat tttatgccgc ttcggacctt
     1681 aaacaccaaa aaaagtgtcg ttccctcatc aagctattcg ccttcacggg taagcttatc
     1741 attcagtttg aaattccggc cgtgaatttt tctgtatttt tcaatatact cggatggttt
     1801 catgccaaat gtgcgggtaa actgctcacg gaagtatttg atatccttaa tcccaacata
     1861 aaaagctgtt tcattaatat tataatcaga attaataaac agctcagcgg ctttccttaa
     1921 acggatatac cggataaagg cattcgcgct ctgcccggaa atggttttta cttttttgta
     1981 taacttactg tgactcattc ccatatcttg agccaacacc tggatattga agtcatcctt
     2041 atccaggttc ctttccaccg cagcgataca ggactcgagg aactccttat actcgccgga
     2101 aatattcagg gtgttttgct gatgggtaat ttcattatag aaatatttct ggagattttg
     2161 ttgctttttg atcaggcttt gaaccctggc caccagcata tccttttcaa acggcttggt
     2221 aatatagtca tctgcccctg actcgatccc tttcagtttt gattcctggg aaaaactgcc
     2281 ggtgatcagc acaaagggga tatggctcag ggcggctgtg tcttttacca ttttgcaaaa
     2341 ttcaatcccc gatccgtttt ccatcaccac atcactgata atgatatcgg gcatcaactc
     2401 ggcagccatt tttattgctt cttccccact accggcttca tacacgataa agtcctcctt
     2461 aaacacctgg gccaggtaac tcctcatctg cggattatca tcggttatta atatggagtg
     2521 cttttcactg accaccgact ccaatccgtc agtttgccat ttcaaaacag gcaccggttc
     2581 ctccccggca ataatctcat ccagcaactc atgttcagac tctccggagg actccccgac
     2641 cggcacatcc ccgaaatgct gatatccggt cttgagggcc ataaaaaaac gggtaccttt
     2701 tccgggcgta ctttcatacc ctatttgtcc cttgtgttcc tccaccagtt gcttaacaag
     2761 ataaagcccg attccaaatc cggccttggc ctttgcttct tttttattga cctggtaaaa
     2821 tttttcaaat agacggctac ccgtttccgg aggtatcccg ggtccggaat cggccacttc
     2881 tgccaacacc ttgtctcctt cttcccaaat gcggaaacta acttgtccat cagccggcgt
     2941 gtatttaagg gcattggaca acagattata aaaaacaatc tgtagttttt cacggtcacc
     3001 ataaacagcg ggtaactgat cgggacattc gtaggtatac cgtatgcctt ttgacctggc
     3061 ttgctgaata aagaaggaaa aagtttcttc tcccatctgc cgggtatcga gcctggctac
     3121 atgcaattta cctgtctcgc tttccgtttt cctgaacaat aataactgat ccaccaggct
     3181 aagtaaacgc ctggcgtttc ggtacaccag ttctatgtcc gcactttctg tttttggctt
     3241 gccatttacc aggtccttaa gcggattgat gatcagggtc agcggggttc tgaactcatg
     3301 agtaatattg gtaaaaaaag actgcctttt ctcattgatc tctttttctt tttcagcatt
     3361 cagctccgtc agtttcattt tatatttcag gtgcgcctgc tgggagcggt accggagata
     3421 caaccagatc agggacccgg cggccatcat atatataagg taagcccacc agctgcggta
     3481 ccagggtgga aggatcacca gtttcaacaa ggtttcctgt tcattccact gaccgttgac
     3541 attagtgctc ttcaggtgaa gggtataact gccttctgcc aggttgttgt agttaatatt
     3601 atttatatta ccggaatagt tccagttctt atcccagcct tcgaggaaat agccatactt
     3661 tattttttcc ggtgaagtat actccaatgc cgtaaaactg aaattgaaaa cggcctgatc
     3721 aaaaggaacg accattttgg cgatatcatt atttctaatt ggacggatga agcgactaag
     3781 ttctgcaaca ggcttcccgt taaccaggat attggttata tagaccggag gcatgtacgc
     3841 ccggggaaca atctgttccg gtttgaacac gctgaatccg tttataccac caaacgccaa
     3901 ctcaccattt ctgagttgtg tggcagcccg gtagctgaac tggttactct gtaatccatc
     3961 actctgataa taatttttga aaacacggct tgcaaaatca aactgggata acccgttgaa
     4021 ggtactcatc cacaaatggc cggtactgtc ttccagaata ttcaagaccc cattattaca
     4081 caatccgtcc gcatcggaaa agcgctctat caattgtcct ttctcccggt caaataaaag
     4141 taaacctccc ccttccgtac ccaaccagaa acggccggct ttatcttcaa aaatggaacg
     4201 taccgggcgg ccaatcgaga aggaagtatg ttttttctgt aaaaggtcca gtttaacgag
     4261 caaactggag ttgccgaccc ataaattccc tttacggtct tcgagcatag aaatcaggtt
     4321 agttaacccg ggatcgaata attcaaactt atttaaccgc cggtcgaaca ggtaaagctt
     4381 cccgtcatta aatgcagagg cccagatatt atgttgcctg tcttcataca aaagggaaat
     4441 gtttttgttc tcataaccgg tccgttcatc gatgcaggga accggctcaa aacggccttt
     4501 ctgtttatta aaatgactga tccccccgcc ataagtagcc acccagatga tgcctgtatt
     4561 atcctgaagg atccgggtca ctgaattatg gctgagtgat cctttgtctc ccggctgatg
     4621 attatagttg gtaaactggt tagttacccg gttccagaca cttagaccgc caccatccgt
     4681 accaatgagc agatcgcggt cgcggtcttc acaaaaggaa taaacagtac tgaccgggag
     4741 tgtgttatta ctgaaaggct gacgtgagat actggtaaaa cggccctttt gtacatcaat
     4801 aatattacac cctcccttga tagtaccgat ccagatgcgg gactccttgt cccttaatat
     4861 ggaactgacc gtttcactgg aaagcttgtc agagccatcc cctgattcaa tatagcggaa
     4921 ttgctttgtg gcccggtcaa ctaaagtaat cccgccccga tctgtcccaa tccatagctg
     4981 attaaac
//
LOCUS       DAOPMW010000244         4963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19717_length_4963_cov_7.136308, whole genome shotgun sequence.
ACCESSION   DAOPMW010000244 DAOPMW010000000
VERSION     DAOPMW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19717_length_4963_cov_7.136308"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..2777
                     /locus_tag="PLU11_11050"
     CDS             282..2777
                     /locus_tag="PLU11_11050"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPN59705.1"
                     /translation="MKKLLFLLIAACQFTGGKTQAPQNWTSAEMYQALLKLKVLGAVL
                     YVAAHPDDENTRLITYLSKDKLYRTGYLSMTRGDGGQNLVGEEQGIELGLLRTQELLA
                     ARRTDGGEQFFTRAFDFGYSKTPEETFSKWDKEKILADVVWVIRKFQPDIIVTRFPTT
                     GEGGHGHHTGSALLANEAFAAAADPSRFPEQLKYVSVWQTKRVMWNTFNFGGNNTTRE
                     DQFKVDVGGYNPLLGKSYGEIAAESRSQHKSQGFGVPASRGESWEYFRAVKGDQPVND
                     LLDGVDPTWNRVKGGAAVAAMLDSISARFDFIHPEKSVKGLVQLYQLISSLPDHYWKT
                     QKLKEVEELIAQCSGLFLDVTTSAQFAVQTDSVRVSFSLNNRLGTQAVLEAVSISGFD
                     SVLNKKLDKNRNLSFSKTIYVPVERTITQPYWLVAKMEEGSFNVANQQKIGQPDADPA
                     YEAVLNISIYGTSFRFTRPVRYKFTDPVRGELYWPLVVVPPAEFEFTEANNISVNDTT
                     AQTALHFSTNLRKDTSAYHVQLKYPSAKWYAKRQEFTYKPWPDNSSNAAFFNLRPVTK
                     NKNSTDSLSVTVIQNTKPIYAGERKQVKYDHVPSIIYFKDAKANLVQIDLKTYGKKIG
                     YITGAGDKVPEALEQMGYEVIPLGDKELTRNNLAQFDAIITGVRAYNTHAWMNKHFDK
                     LMKYVNEGGNLIVQYNTSNQIGPVRAKIGPYPFTITRNRVTDENAPVRMLNPEHPVFS
                     FPNKITAEDFTGWKQERSIYHASDTSGKFEKLIGMADPGEKSDDGSLLVAKYGKGWFT
                     YTGLVFFRELPAGVPGAYRLLANIIALNKKKGF"
     gene            2777..2956
                     /locus_tag="PLU11_11055"
     CDS             2777..2956
                     /locus_tag="PLU11_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59706.1"
                     /translation="MPEEKKGGIRRSEMAFLVAIVLGLVLGILIKKIRIGILVGLVLG
                     FVIVLTGWLRTTRKK"
     gene            3081..4817
                     /locus_tag="PLU11_11060"
     CDS             3081..4817
                     /locus_tag="PLU11_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPN59707.1"
                     /translation="MSLADWIVLVVTLLSIVAYGLYKSRTSRNLDGYFLSNRSMPWGL
                     VLLSIMGTQASAITFLSAPGQAYTDGMRFVQYYFGIPLAMVVISIFFVPIFHRLRVYT
                     AYEYLEQRFDGKTRTLTSLLFFLQRGLSTGISVFAPSIILSSLLGWDIIWTNLFMGGL
                     LIIYTMSGGAKAVAYTQQIQLFIVFTGMFLAAYMVVKMLPADVGFADALQVGGKLGKL
                     NIISTGFTEKGFNWSDQYNLLSGIIGGFFLALSYFGTDQSQVGRYLTAKSLKESRMGL
                     LMNGIVKVPMQFLILLIGVLVFTFYQFNNAPIFFNEVQLKKLEASDYKDSLKIVQGHY
                     AAVAVNKQQAVRDYTMARHDGEEDKETASLASLQSLQKESDSLRKKVVYWLNKKVPGG
                     DGNDTNYIFLRFVVDHLPVGLVGLLIAIIFLASWGSIAAAINSLASCSMVDFHRRFTK
                     KKDSGEDEYRLSKWYTLGWGVFCIIVAMFTYNIGNSLIEAVNILGSLFYGVILGVFLV
                     AFFFKKITNSNPVFWGAILAQIMVLFVFILTKVGVFQMGFLWLNPIGAFGVVGFTLLL
                     DQLFRRKLEARG"
BASE COUNT         1312 a         1074 c         1252 g         1325 t
ORIGIN      
        1 acagccgaca gccgacagcc gacagccaac agccgacagc cgacagccga cagccgacag
       61 ccgacagact cacttattag cgattttttt aataacaccg tggaggaaca ttttcttccc
      121 ggtgtttttt ttagttaaat ggagtttatg ttgtctctcg gcttttgatt atttgtcatt
      181 cggatatccc cacttccggt cttcccggtt tacccccttc cggatattca ggcttcccga
      241 cttccgtact ttccgatata ttcgctgaat aatactatcg gatgaaaaaa ttgctttttt
      301 tactcatagc ggcctgccag tttacagggg ggaaaactca ggcaccgcag aactggacat
      361 cggctgaaat gtaccaggcc ttactgaaac tgaaagtgct gggtgcggtg ttatatgtgg
      421 ccgcccaccc ggatgatgag aatacccggc tgattacgta tttatcaaaa gataaactct
      481 atcgtacagg ctatctgtcc atgacccgcg gggatggcgg acaaaacctg gtgggagagg
      541 aacagggtat tgaactgggt ttgctacgta cacaggaatt gctggctgcc agacgtacag
      601 acgggggcga acaattcttt acccgggctt ttgatttcgg ctattcaaaa acacctgaag
      661 aaactttctc taaatgggat aaggaaaaga tacttgcaga tgtggtgtgg gtgatccgca
      721 aattccaacc ggatattatc gtgacccgtt tccccacaac cggtgaaggc ggacatggac
      781 accataccgg atcagcctta ctggccaatg aagcttttgc ggcggcggcc gatccatcgc
      841 gttttccgga acaattgaaa tatgtatccg tctggcagac caaacgtgtc atgtggaata
      901 ccttcaattt cggaggcaac aataccaccc gtgaagatca gtttaaagtg gatgtaggcg
      961 gttataatcc attgctagga aaaagttatg gagagattgc cgccgaaagc cggagccagc
     1021 ataagagcca gggcttcggg gtgcctgctt cccgggggga gtcctgggaa tatttcagag
     1081 ccgtgaaagg cgaccaaccg gtcaatgatt tactggatgg agtagatcca acctggaacc
     1141 gcgtaaaggg tggggcagcc gtagcggcga tgctggacag tatcagtgcc cgctttgact
     1201 ttatacatcc tgaaaagtct gtcaagggac tggtgcagtt ataccagctg atcagttcac
     1261 tgccggatca ttactggaaa acccagaaat taaaggaagt ggaagaactg atcgcacaat
     1321 gcagcggatt attcctggat gtcactacca gtgcacagtt tgcggtacaa acggattcag
     1381 tacgggtcag cttcagtctg aataaccgcc tgggtactca ggctgtactt gaagcggtca
     1441 gtataagtgg ctttgattct gtattaaata agaaactgga taaaaacagg aatctcagtt
     1501 tttcaaaaac aatttacgtt ccggttgaaa ggactatcac ccagccgtat tggctggtgg
     1561 ccaaaatgga agaaggatct tttaatgtgg ccaatcagca gaaaatcggg caaccggatg
     1621 ctgatcccgc ttatgaggct gtgttgaata tcagcattta tgggacgtca ttccgtttta
     1681 cccgaccggt tcggtataaa ttcacagacc cggtaagggg ggagctttat tggcctttag
     1741 tagtagtgcc tcccgctgag tttgagttta cggaagccaa taatatttct gttaatgata
     1801 ctaccgcaca aaccgctttg catttcagca ctaatctgcg gaaagatact tctgcctacc
     1861 atgtacaact gaaatatccg tcagcgaagt ggtatgcgaa acggcaggag tttacttata
     1921 agccctggcc tgataacagc agtaatgctg cttttttcaa cctgaggcct gttacaaaga
     1981 ataaaaacag tacagatagt ctttcagtaa cggttattca aaacactaaa cccatttatg
     2041 ctggcgaacg aaagcaggta aaatatgacc atgtgccctc aatcatttat tttaaggatg
     2101 cgaaagccaa cctggtacaa attgatttga aaacttatgg gaagaagatc ggttatatta
     2161 ccggtgccgg tgataaagtg cctgaggccc tggagcaaat gggttatgaa gtgattccgc
     2221 tcggggataa ggaactgacc agaaataacc tcgcccagtt tgatgctatc attaccgggg
     2281 tgagggctta taatacacat gcctggatga acaagcattt tgataagctg atgaagtatg
     2341 tgaacgaagg gggaaacctg attgttcaat ataacacgag taaccagata ggtccggtaa
     2401 gggcaaagat cggtccttat ccctttacca ttacccgcaa ccgggtgacc gatgaaaatg
     2461 cgccggtacg gatgttgaat cctgaacacc cggtcttttc ttttccgaat aaaattacgg
     2521 ccgaagattt caccggctgg aagcaggagc gtagtattta ccatgccagc gatacatccg
     2581 gtaaatttga gaaactgatt ggtatggctg atcccggtga gaaatcagat gacggcagtc
     2641 tgctcgtggc taaatatggc aagggttggt tcacgtatac cggcctggtc tttttccgtg
     2701 aattgcctgc tggcgtacct ggagcctacc gcttactggc aaatattatt gcgttaaaca
     2761 agaaaaaagg attctgatgc cagaagagaa aaaaggcggg atccgccgga gtgaaatggc
     2821 ttttctggta gcaatagtcc ttggacttgt actggggata ctgatcaaga aaatcagaat
     2881 cggtatcttg gtcggcctgg tacttgggtt tgttattgta ctgaccggct ggttgcggac
     2941 cacacgaaaa aaataactat gaaagaatcc gataacgaaa aagccccctt tttcaaaagc
     3001 tggaatagct ggtatgtact ggttatagga tttctgtttt tgttgatcat cctgttctac
     3061 ttaattacta aacgatttgc atgagcctgg cggactggat agtactggtt gttaccttgc
     3121 tgagtattgt ggcttatggc ctctacaaaa gccgtacttc gagaaatctc gatgggtatt
     3181 tcctgagtaa ccgcagcatg ccctggggac tggtcttact cagcatcatg ggtacacagg
     3241 ccagtgcgat tactttttta tcggcccccg gacaggcata tacggatgga atgcggtttg
     3301 ttcagtatta ttttggcatt cccctcgcca tggtggtgat ctccatcttc tttgttccga
     3361 tctttcaccg gctccgggtg tatacggcat atgaatacct ggaacaacgc tttgatggta
     3421 aaaccagaac gcttacctcc ctgctttttt tcctgcagcg gggcctgtca acagggatca
     3481 gtgtatttgc cccttcgatc attttatctt ctttattggg atgggatatt atctggacca
     3541 atctcttcat gggcggacta ctcatcattt acaccatgag cggcggagcc aaggccgtag
     3601 cctataccca acagatccag ttattcattg tattcaccgg gatgtttctt gctgcgtaca
     3661 tggtagtgaa aatgctccct gctgatgtgg gttttgcgga tgccctgcaa gtggggggca
     3721 aactgggcaa actgaatatt atcagcaccg gtttcacaga gaagggattc aactggagtg
     3781 atcagtataa tttgctaagc gggatcatcg gcggtttttt cctggccctg tcttatttcg
     3841 gtaccgacca gagccaggtg ggccgttatc tcacggccaa atcactgaag gaaagccgga
     3901 tgggattact gatgaacggg atcgtgaaag tgcccatgca gttcctgatc ctgctgatcg
     3961 gggtattggt ctttacattt taccaattta acaatgcgcc catttttttc aatgaggtac
     4021 aactgaaaaa actggaagcc tcggactata aggattcatt gaaaatcgta cagggtcatt
     4081 atgccgctgt agcggtgaat aaacaacagg cagtgcggga ttataccatg gcccgacatg
     4141 acggtgagga agataaagaa acggcttcac tggcctcttt acaatcctta cagaaagaat
     4201 cggacagtct gcggaagaaa gtcgtgtatt ggctgaataa aaaagtgcct ggcggtgacg
     4261 ggaatgatac caattatatt ttcctgcgct ttgtggtaga tcatttgccg gtaggactcg
     4321 tagggctgct catcgcgatc atctttctcg cttcctgggg cagtattgcc gcggcaatca
     4381 attcgctggc ttcctgttcc atggtggatt ttcaccgccg gtttacgaag aaaaaagaca
     4441 gcggtgagga tgaataccgc ttatcaaaat ggtatacact gggctggggt gtgttctgta
     4501 tcatcgtggc catgtttacg tataatatcg ggaacagcct gatcgaagcg gtgaatatcc
     4561 tgggctcttt attctacgga gtgatcctgg gggtgttcct ggtggcattc ttcttcaaaa
     4621 aaatcaccaa tagcaatccg gtgttctggg gcgccatcct ggcacagata atggtgttgt
     4681 ttgtatttat cctcacaaag gttggcgtat ttcaaatggg cttcctctgg ctgaatccga
     4741 tcggggcgtt tggggtggtt gggttcacgt tacttttaga tcagttattt cggaggaagc
     4801 tagaagctag aggctagaag cgagaagggg catttcgtaa aacgaggaac atttcatgtt
     4861 ttaagaataa tattctaaga aggggctgtt gacctaacac ctatcagcta acacctaaca
     4921 ccagcataaa atacaatgtt cttcgctaaa aatacaaggt ccc
//
LOCUS       DAOPMW010000245         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19824_length_4942_cov_10.208103, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000245 DAOPMW010000000
VERSION     DAOPMW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19824_length_4942_cov_10.208103"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="ftsA"
                     /locus_tag="PLU11_11065"
     CDS             complement(<1..1196)
                     /gene="ftsA"
                     /locus_tag="PLU11_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPN59708.1"
                     /translation="MNSEQPIIVGLDIGTTKIAVIAGRKNEYGKLEILGFGKANSNGV
                     KHGQVLNIDETIKAIRTALDNCFASNPDLDISEVYVGIAGHHIKSLQTRGDIVRQRTD
                     EEITQREIDQLIDDQYKTYIPAGDQIIDVIPQEFTVDNFQNIPNPIGYGGVKIGANFH
                     IITGDKNAIRNINRSVEKSGLRTKDLVLQPLASSSAVMGQEDVEAGVAIVDIGGGTTD
                     LAVFYEGILKHTAVIPFAGENITNDIKTGLGVLKTQAEQMKVQFGSALANEAKANAYI
                     TIPGLRGMPAKEISVKNLANIIQARMSEIMDFVTYHLKQVGLDNKALNGGIILTGGGS
                     QLKHLIQLTEYVTGLNARIGYPNEHLAGGHIEELSKPTYSTCIGLILKGYDDYEHNRK
                     EFDKDFR"
     gene            complement(1240..2346)
                     /locus_tag="PLU11_11070"
     CDS             complement(1240..2346)
                     /locus_tag="PLU11_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPN59709.1"
                     /translation="MSAKKTIRKIIFVTVWLCIGAGMFTLLMAAITRKNNGHCADYSI
                     RIKGVEKKYFIDEKDVEALLKKAAGGAIKGQPLTDLNLHDMEVTLERNTWISDAELYV
                     DNQDVLHVMIVEEEPVARIFTSTGNSFYIDKTGKKLPLSDKISARVPVFTGYPDLKKM
                     NKADSALLAKVTTAANYIVADPFWMAQVAQMHITPERNFEMIPVVGNHQVKMGAGEQI
                     DRQFGRLMIFYKEVLSKTGFDRFKVIDVQYKGQVVASKTVGNTKVDSVQLRRNVEMLL
                     QQSRQAENDTVARLLPVAAIPLETDAPDESPIVESPDKPKTLPTNTNPVPLKSPVPAD
                     RKVQPKPVKQNKPALKQPKAVMPPKGEDPDNGYN"
     gene            complement(2348..3745)
                     /gene="murC"
                     /locus_tag="PLU11_11075"
     CDS             complement(2348..3745)
                     /gene="murC"
                     /locus_tag="PLU11_11075"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPN59710.1"
                     /translation="MNTPFGGIDNLKEVYFIGIGGIGMSAIARFFQSGGVKVSGYDKT
                     PTPLTRELEASGIAVHYEENTVLIPKTPDLIVYTPAVPAAHAELVYYRENGFSVVKRS
                     DVLQLISKSSFNICIAGTHGKTTITTMTAHLLRDSGFGCNAFLGGIAVNYGSNFWSSE
                     MNVCVIEADEYDRSFLKLSPDVAVITAMDADHLDIYGTAEAMEDAFIGFSKAIKPGGL
                     LIRQFGLKRGKELQASQHFTYSLQNEAADLYATNIRMMNGSYEFDVVLQEEVIENVKL
                     NMGGMHNVENAVAAIAIVSSLGIDKEKIKTAVENFRGVKRRFEYILKNDRIVFIDDYA
                     HHPEELRALINGAKTLFRQKKCTVLFQPHLFSRTRDLAEGFAEVLDLADEVVLLPIYP
                     ARELPVEGVNSEMVLKRMQIGNKAVMDKEALMDWIKQHYLPGVNKEFGAVLVTAGAGD
                     IDNLIEPIKNLLKEI"
     gene            complement(3854..4942)
                     /gene="murG"
                     /locus_tag="PLU11_11080"
     CDS             complement(3854..4942)
                     /gene="murG"
                     /locus_tag="PLU11_11080"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPN59711.1"
                     /translation="MSKRIIIAGGGTGGHIFPAIAIANALKKKDPSIEILFVGAKGKM
                     EMEKVPQAGYKIEGLDIAGFNRSSLIKNIGLPFKLVKSFLQVRRIFNQFRPDAAIGVG
                     GYSSFPVLKTAQTKGIKTFIHESNSFAGKSNILLGKKATRVFTGTDGMEKFFPADKIR
                     VTGNPVRVAITRASVTREEGINFFGLDPQQKTLFVVGGSLGARSVNEAIGAGLEELLG
                     AGLQLIWQTGKPYANTAKELAKGKSGVWVNEFITQMEFAYAAADIVVARSGAMTVAEL
                     CVARKPVVFVPYPFAAEDHQTVNAQQLVNKKAAMMVKDNEVMQVLVATTIGLAKDEAR
                     QQEMKANIASMAITNADEVVADEILKLI"
BASE COUNT         1108 a         1307 c         1071 g         1456 t
ORIGIN      
        1 cggaagtctt tatcaaactc tttccggttg tgctcataat catcataccc tttcaggata
       61 agtccgatac aggtggaata agttggcttg gagagttcct caatatgtcc acccgccagg
      121 tgctcattgg gataaccgat cctggcattg agccctgtta catattcagt cagctggatc
      181 aggtgtttca gttgcgaacc accacccgtg aggatgattc ctccattgag tgctttattg
      241 tccagtccca cctgtttcag gtgataggtc acaaagtcca tgatctcact catacgcgcc
      301 tggatgatat tggccaggtt cttcacgctg atttccttgg caggcatgcc gcgtaaaccg
      361 gggatggtga tataggcatt ggctttggcc tcattggcaa gagccgaacc aaactgcact
      421 ttcatttgct cggcctgtgt tttcaaaaca cccagaccgg ttttgatgtc gttggtaata
      481 ttttctcccg cgaatggaat taccgcggta tgttttaata tgccttcata gaagacagcc
      541 aggtcggtgg ttccaccacc gatatccacg atcgccacac cagcttccac atcttcctgt
      601 cccatgacgg cagaagaaga agcgaggggt tgcaacacga ggtccttggt acgcagaccg
      661 gatttttcca cactgcggtt gatattgcga atggcattct tatcaccggt aatgatgtga
      721 aaattggcgc cgatcttcac cccaccatac ccgatcgggt tggggatgtt ctggaaatta
      781 tccacggtga actcctgggg gatcacatca atgatctgat caccggcagg aatataagtt
      841 ttgtactgat catcaatcag ctggtcgatc tcccgttggg tgatctcctc gtcggtccgc
      901 tgacggacaa tgtcgccacg ggtctgaagg ctcttgatgt gatggccggc aataccgaca
      961 tagacttcgc tgatgtcgag gtcaggattg gaggcaaagc aattgtcaag ggctgtacgg
     1021 atggctttga tggtttcatc gatgttgagc acctgtccgt gctttacacc attggagttg
     1081 gcttttccga atcccagaat ctcgagtttt ccatactcgt tcttccggcc ggcaattacc
     1141 gcgatcttag tggtaccgat gtcaagaccg acaataatgg gttgttcgct gttcatagtt
     1201 gttgttttag tagttgtttt tcgaaatcgt tatgttgttt tagttgtatc cattgtccgg
     1261 gtcttctcct tttggcggca ttaccgcttt gggttgttta agggccggtt tgttttgctt
     1321 tactggtttt ggctgaactt tcctgtcagc cggaacagga gatttcaaag ggacgggatt
     1381 ggtattcgtt ggtaatgttt tcggtttgtc cgggctttcc acgatgggtg attcatccgg
     1441 tgcatctgtt tcaaggggga tggccgcaac gggcaataat cttgccacag tatcattttc
     1501 ggcttgtctt gactgttgta aaagcatttc cacattccgg cgcaactgaa cggaatccac
     1561 tttggtattt ccaactgtct ttgaagccac cacctgtcct ttatattgca catcgatcac
     1621 tttaaaacga tcgaatcctg ttttactcag cacctctttg taaaagatca tcagtcggcc
     1681 aaactgccgg tcaatctgtt cccccgctcc cattttcacc tggtggttac cgaccaccgg
     1741 tatcatttca aaattccttt ccggggtgat atgcatctgt gccacctgtg ccatccaaaa
     1801 aggatccgcc acgatatagt tggcagccgt cgtcactttt gccagtaatg cactatccgc
     1861 tttattcatt ttcttcaggt ccgggtaccc ggtaaagaca ggcaccctgg cactgatttt
     1921 atctgaaagc ggcagtttct tgccggtttt gtcaatataa aagctgttgc cggtgctggt
     1981 aaaaatgcgg gcaaccggct cttcttccac aatcattaca tgcagcacgt cctggttatc
     2041 gacatacaat tcggcatcac tgatccaggt attgcgttcc agggtcactt ccatgtcatg
     2101 cagattgagg tccgtaagtg gctgcccctt tattgcgcct ccggcggctt tcttcaacag
     2161 ggcttccaca tctttctcat caataaaata tttcttctcc acccctttga tccggatcga
     2221 ataatctgca caatgcccgt tgtttttgcg ggtgatcgcg gccatcagca gggtaaacat
     2281 acccgccccg atgcacagcc ataccgtgac aaaaatgatt ttccgtatcg ttttctttgc
     2341 gctcaattta tatttccttc aacaggtttt taatgggttc aatcaggttg tcaatatctc
     2401 ctgccccggc ggtgaccagt actgcgccaa attctttatt cactccgggc aggtaatgct
     2461 gcttaatcca atccatcagg gcctctttat ccatcaccgc tttattcccg atctgcatcc
     2521 gcttcagtac catctcactg ttcactcctt ctaccggcaa ttcgcgggcc gggtagatgg
     2581 ggagcaggac tacttcatca gccaggtcca gtacttccgc aaatccttcg gccaggtcgc
     2641 gggtgcggct gaacaaatgc ggttgaaaga ggaccgtaca ttttttctgc cggaacaggg
     2701 ttttcgcccc gttgatcagc gccctcagtt cttccgggtg gtgggcataa tcatcaatga
     2761 atacgatccg gtcatttttc aggatatatt caaagcgtct tttcaccccg cggaaatttt
     2821 ctaccgctgt tttgatcttt tctttatcaa ttcccaaaga actgacaatg gcaatcgcgg
     2881 ccaccgcatt ttccacattg tgcatccccc ccatgttcag tttcacattt tcaatcacct
     2941 cttcctgcag caccacatca aattcatagc tgccattcat catccggata ttagtggcat
     3001 agaggtcagc agcttcattt tgcaggctgt acgtaaaatg ctgactggcc tgcaattcct
     3061 ttcctctttt caatccaaac tggcggatca gtaatcctcc cggttttatt gctttgctga
     3121 aaccgataaa cgcatcttcc atcgcttccg ccgtgccata gatatcgagg tgatcagcat
     3181 ccatggccgt gatcaccgcc acatccggac tcagtttcaa aaagctgcgg tcatattcat
     3241 cggcttcaat cacacataca ttcatttcac tgctccagaa attcgaaccg taattcaccg
     3301 caatgcctcc taagaaagca ttgcatccga aaccactgtc gcgcagcaaa tgcgcggtca
     3361 tggttgttat ggtcgttttg ccatgggtac cggcaataca gatattaaaa gaacttttac
     3421 tgatcagctg taatacatca cttcttttta ccacactaaa tccattctcc cggtaataaa
     3481 ccagttccgc atgcgccgcc ggaacagccg gtgtatacac aatcagatcg ggggtctttg
     3541 gaatcagtac cgtattttct tcatagtgta ccgcgattcc gctggcttcc agttcccggg
     3601 tcaggggcgt cggggtttta tcatacccgc tcacttttac tccaccagac tggaagaaac
     3661 gggcgatggc actcatgccg attcctccga ttccgataaa gtagacctct ttcaggttgt
     3721 caatcccccc aaagggggta ttcataagga cagtattggc ttgtacggtt ttcccacctg
     3781 cacctggaaa agtgcctgga gagaatgttt ttattaaaaa atcaattttc aaaaggttcc
     3841 gtctatatag ttttcagatc agtttcaaaa tttcatccgc caccacttca tccgcattcg
     3901 ttatggccat tgaggcaata tttgctttca tttcctgttg tctggcttca tcttttgcca
     3961 gcccgatggt tgttgcaacc agtacctgca ttacttcatt atcctttacc atcatcgcag
     4021 ccttcttatt caccaattgt tgtgcgttca cagtctgatg atcttcggcc gcaaacggat
     4081 agggcacaaa cactacgggc tttctggcga cacataattc agctaccgtc atcgcccctg
     4141 aacgtgcgac cactatatca gccgccgcat aggcaaattc catctgggtg ataaattcat
     4201 tcacccatac cccgcttttc cctttcgcta attcttttgc ggtgttggca taaggctttc
     4261 cggtttgcca gatcagttga agacctgcac caaggagttc ttccaatccg gcacctattg
     4321 cttcattgac ggatctggcc ccgagacttc ctcccaccac gaacaaggtt ttctgctgcg
     4381 gatccagtcc aaagaagttg attccttctt cgcgggtaac gctggcccgg gtgatcgcca
     4441 cccttaccgg attaccggtg acccttattt tatccgccgg aaaaaacttt tccattccat
     4501 ctgtaccggt aaatacacgg gttgcttttt tacccagcag gatattcgat ttcccggcaa
     4561 atgaattcga ctcatggata aaggttttga ttccctttgt ttgtgcggtt ttcaaaaccg
     4621 ggaaactgga ataacctccc accccgatgg cggcatccgg tctgaactga ttgaagatgc
     4681 gccgcacctg caaaaagctt ttaaccagtt tgaatggtaa ccctatattt tttatcaaag
     4741 agctccggtt aaacccggca atatccaatc cttcaatttt ataacccgcc tgcggtactt
     4801 tttccatttc catctttcct ttcgctccca caaacaggat ctcaatggaa ggatcttttt
     4861 tcttcagggc attggcgatg gcgatagccg gaaaaatgtg accgcctgta cccccgcccg
     4921 caatgatgat ccgtttgctc at
//
LOCUS       DAOPMW010000246         4940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_19837_length_4940_cov_10.470215, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000246 DAOPMW010000000
VERSION     DAOPMW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19837_length_4940_cov_10.470215"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLU11_11085"
     CDS             complement(<1..494)
                     /locus_tag="PLU11_11085"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN59712.1"
                     /translation="MLLLYIVLGLFAVLLIAASLLPKSYNVEKNIIIKRPADYVMQRI
                     SDLNYYSQWNPWQQSDPSAKSTITGTAGLSGHKYAWEGKKVGIGSLTLLSMDAKHINF
                     DLQFIKPMNSKASDNWLFESWGDGNETKVTWQNSGELPFPIGRLMGPMISGQLNKQFV
                     QGLE"
     gene            complement(554..1117)
                     /locus_tag="PLU11_11090"
     CDS             complement(554..1117)
                     /locus_tag="PLU11_11090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN59713.1"
                     /translation="MLIGACSGPKQTYSVRDLVNGKFKADSSFIYSIPFKTGKNVFVI
                     QGYETPFSHKGERALDFKVKTGTLVCAAREGIVTSVRKDSDKGGLKPEYLSEGNYVII
                     RHADSSTAWYWHFKKDGVLVNEGDMVTDGQPIGYSGNTGYSAFPHLHFEVQGTDAAGN
                     YTQLATRFYTREGAIYLRPLRFYRTRR"
     gene            complement(1241..2284)
                     /locus_tag="PLU11_11095"
     CDS             complement(1241..2284)
                     /locus_tag="PLU11_11095"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPN59714.1"
                     /translation="MPATKAYAAHSANTPLEPWSLERRAPKPNDVEMEILFCGVCHSD
                     LHFARNEWGMTVFPIVPGHEIVGRVTAVGDHVKKFKAGDLAGIGCLVDSCRECDNCKE
                     GLEQYCAQGGVFSYNGFERDGSGITYGGYSKSIVCHEDFVLRISDKLDLAATAPLLCA
                     GITTYSPLRHWKVGKGMKVGILGLGGLGHMAVKFAVSMGAEVTMLSHSPSKETDAKRL
                     GAHKFILTSNEEQAKSVMGSLDFILDTVSAPHDYNFYLGMLRTNGVMVCVGVPPEPVP
                     LHPMSLIFGRKSLGGSLIGGLPETQEMLDYCAEHNIVSDIEMIRMDQINEAYERLLKS
                     DVKYRFVIDMATL"
     gene            2514..3194
                     /locus_tag="PLU11_11100"
     CDS             2514..3194
                     /locus_tag="PLU11_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59715.1"
                     /translation="MKKLLFLSLALLICSALLAQGSGNTVNKHSFLAFHAGPSFPVGA
                     FGSNNPGNDDAGLAKTGYTIAMNYGYRLNSPLGLEAAVFYNHFGTQKMVLTVDVGDGT
                     RTIDMTLDDWHLYGISVGPSLEFSLAKKMSAGLHVMGGVANVRLPAFYFDGEDATKAD
                     WGIGPVVQAGLNLKIDVGKQVFFFVNGDYQFMRPVFNVLDLWTDESEKAYQKISVLQA
                     SAGIGFRF"
     gene            3327..4511
                     /locus_tag="PLU11_11105"
     CDS             3327..4511
                     /locus_tag="PLU11_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN59716.1"
                     /translation="MTDNSNLSAASKRTMRIAVSAFYFCMGFSFASWASRIPDIKAKL
                     HMNEAALGTVLLCLPVGQLLMMPFSGRLVTKYGSKKILSFAIVLYTLELTNIGWATEM
                     WQLGLALFIFGIVGNMSNISVNTQGVLAQNIYGRPIMTTFHGIWSLSGLTGALVGWLM
                     VNLKVPPRPHFFIVAGLVLITVFIAWKYLIAGTSSPAGNSKLFSKPEGVLVKLGIIAF
                     CSMAAEGAMFDWSGVYFREVVKAPVSMVILGYTSFMIMMTAGRFMGDQLIAKIGRKKT
                     LQISGILVFTGLTISVLFPWVVTAAIGFMIVGLGASSVIPTIYSTAASASKKSPGMAL
                     AGVSSIGFLGFLFGPPLIGYIAQLFSLRTSFGVVAIFGLLITLMVTKLKVMDDVKLIK
                     TA"
     gene            4514..4876
                     /locus_tag="PLU11_11110"
     CDS             4514..4876
                     /locus_tag="PLU11_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPN59717.1"
                     /translation="MKLDLAAFRPINVQRATEGFKCYDNQPLTYWTTALAGEVGELCN
                     MIKKMQRVERGGVDGGSSYTAKDITKEMLKEEIGGIAIYLDLLASLLDISLEEAIVDT
                     FNSKSEKYGFKEKIVVSS"
BASE COUNT         1264 a         1196 c         1139 g         1341 t
ORIGIN      
        1 ctctccaagc cctgtacaaa ctgcttattc agctgaccgc tgatcatcgg acccatcagg
       61 cggccaatgg ggaagggcag ttcgccgctg ttttgccagg taaccttggt ttcgtttccg
      121 tcgccccagg attcaaagag ccagttatcg ctggctttgc tgttcatggg tttgataaac
      181 tgcagatcga aatttatatg ctttgcatcc atgctcagca gcgtaagact cccaataccg
      241 actttcttcc cctcccaggc atatttatgt ccggatagtc ctgctgtgcc ggttatggta
      301 cttttagcgc taggatcgga ttgctgccag gggttccatt gactgtaata attgaggtcg
      361 cttatgcgtt gcatcacgta atcggctggt cgtttgataa taatattctt ctctacattg
      421 taggatttgg gaagcaggga ggcagcgatc agtaacacag caaataagcc gagaacgata
      481 taaagtaaaa gcatggcaaa cggtttagac tgttaattta agaaattcct gagggctgcc
      541 cgtgattatt ccatcagcgg cgggtgcggt aaaaacggag gggacgaaga taaatggctc
      601 cttcccgggt atagaatcgg gtggccagct gtgtatagtt gcctgcagca tccgttccct
      661 gtacttcaaa atgcaggtgg ggaaaagctg aatagccggt attgccacta tacccaatgg
      721 gttgtccgtc tgtcaccata tccccttcat tgaccaacac cccgtctttt ttaaaatgcc
      781 agtaccaggc cgtactgctg tctgcatgcc ggataatcac ataattgcct tcagacaggt
      841 attcgggttt cagcccccct ttatccgaat ccttccttac tgatgtaaca attccctccc
      901 gcgccgcaca gaccagggtt cctgttttca ctttaaaatc gagtgcacgt tctcccttat
      961 gactgaatgg ggtttcatac ccctggatca cgaatacatt ctttcctgtc ttgaagggga
     1021 ttgaataaat aaaactgctg tccgctttga atttaccatt gacaagatcc cgcaccgaat
     1081 acgtctgctt tggaccactg catgccccga tcagcaaaca aaaagggatt atatatttaa
     1141 aaaaagagaa ggacacaaaa aagatagacc tgtaatatac aaaacagaat agaaaaaggg
     1201 ctgcttcctt atcagaaaca gcccttactt taagcatttt ttataaagtg gccatgtcaa
     1261 tcacaaaccg gtacttcaca tcactcttca ataaacgctc atacgcttcg ttgatctgat
     1321 ccatgcggat catttcaata tcgcttacaa tattatgctc tgcacaatag tccagcattt
     1381 cctgtgtttc cggcaaacca ccgatgagtg atccccccag gctcttgcgg ccgaaaataa
     1441 gactcatggg atgtaacggc accggctccg gcggcacacc cacacagacc atgaccccgt
     1501 tggtacgcag catgccgaga tagaaattat aatcatgcgg tgcggaaacc gtatcaagaa
     1561 taaaatctaa agaacccatc acggatttgg cctgctcctc attactggtg aggataaatt
     1621 tatgggcacc caggcgtttt gcatctgttt ctttcgaagg gctgtgactg agcatggtca
     1681 cttctgcacc catgctgacc gcaaacttaa ccgccatatg tccaagacca cccaggccca
     1741 atatacccac cttcatgcct ttgcccacct tccaatgacg cagtggtgag tacgtggtaa
     1801 tgcctgcaca aagcaatggt gccgttgcgg caagatccag tttgtcagaa atacgcagta
     1861 caaaatcttc atgacataca atgcttttac tgtacccgcc atacgtaatg ccactgccgt
     1921 ctctttcaaa tccgttgtaa ctgaaaacac ctccctgtgc acaatactgt tccagtccct
     1981 ctttacaatt atcacattcc cggcaactgt ccaccaggca accgataccg gcgaggtcgc
     2041 ctgctttaaa tttctttaca tgatcaccaa cggcggttac acgacctact atttcatgtc
     2101 cgggtacaat aggaaaaacc gtcattcccc attcattccg ggcaaagtga agatccgagt
     2161 ggcaaacgcc acagaataag atttccattt ccacatcatt tggtttggga gctctgcgct
     2221 cgagggacca gggttcaagg ggcgtgttgg cactgtgggc agcgtaggct ttggtagcag
     2281 gcatatattt ctttttaatt tgactaaata tacccttatt aaaagagaac caggtattaa
     2341 ttaatcgttt cccggatggc aaactatgaa tgtacatgcg ttgaccaccc gacaacaatg
     2401 atgaatatca actgtcagtt aataactggt catatcagtg ggctttccgg caccgttagc
     2461 ttactgccgg gacacagata atgtgcgatc tgcttacttt ataaatcagc tatatgaaaa
     2521 agcttctctt tttatcgctt gcattactga tttgcagtgc attgcttgcc cagggaagcg
     2581 ggaatacagt aaacaaacat tcctttcttg catttcatgc aggaccttca tttcctgtgg
     2641 gcgcattcgg cagtaataat cccggcaatg atgatgcagg gctggccaaa accggttata
     2701 ccattgccat gaactatggt taccggttaa atagtccgct tggcctggag gctgctgttt
     2761 tctacaacca ctttggcacc cagaaaatgg tgctgacggt agacgtgggt gacggtaccc
     2821 gtaccattga tatgacattg gatgactggc acctctatgg catcagtgtt ggaccttcac
     2881 ttgaattcag cctggctaaa aaaatgagtg caggtcttca tgttatgggc ggggtagcca
     2941 atgtccgcct gcccgctttc tacttcgatg gggaagatgc cactaaagcc gattggggaa
     3001 tcggaccagt ggtacaggcc ggcttgaacc tgaagatcga tgtcggcaaa caggttttct
     3061 tctttgttaa cggggattac cagtttatgc ggcccgtatt taatgtactg gatctctgga
     3121 ctgatgaatc tgaaaaagct tatcagaaaa tatccgtact gcaagcctca gccggaatcg
     3181 gattccggtt ctgagtgtgt gtttgtgagt ccgtctcccg gtcactggcc gggagacttt
     3241 tcaattcatg gagttgtaag gattatcttt gtgccgtccc gtttatggac cagggttcat
     3301 aaaaccaacg aaaacaggag actcctttga cagacaattc caatttatca gctgcctcca
     3361 aaagaaccat gcgcattgcc gtatcggcct tttatttctg catgggtttc agtttcgcct
     3421 catgggcaag ccggattccg gatatcaaag cgaaacttca catgaatgaa gcagcgttgg
     3481 gtacagtgct gctttgcctc ccggtcggac aattactgat gatgcccttc tcggggcgac
     3541 tggtcacgaa atacggcagt aaaaaaatcc tctcctttgc tattgtttta tatacgcttg
     3601 aactgaccaa tatcggttgg gctaccgaaa tgtggcagct cggcctggcc cttttcatat
     3661 tcggcatcgt tgggaatatg tccaatatct ccgtcaatac gcagggagtg ttggcccaga
     3721 atatttatgg tcgtcctatt atgaccacgt tccatggtat ctggagtttg tccggcctta
     3781 ccggcgcgct cgtgggttgg ctgatggtca atctgaaagt tcccccgaga ccacacttct
     3841 tcattgtagc cggtttagta ctgatcaccg tcttcattgc ctggaaatac ctgattgccg
     3901 ggacctcatc tccagccgga aatagcaaac tattctctaa acctgaaggt gtactggtaa
     3961 aactcggtat catcgccttt tgcagtatgg ccgccgaagg agcgatgttt gactggagcg
     4021 gcgtttattt ccgggaagtg gtgaaagcac cggtgtccat ggtcatcctg ggctacacca
     4081 gttttatgat catgatgacc gccgggcgtt ttatgggcga tcagctgatt gcaaaaatcg
     4141 gaagaaagaa aacactccag ataagtggta ttcttgtgtt caccggttta acaatctcgg
     4201 tactttttcc ctgggtagtg actgcagcca tcggctttat gattgtggga ttaggtgcct
     4261 catcggtgat tcctactatt tacagtacgg ccgcgagtgc ttctaaaaaa tcacccggta
     4321 tggccttagc cggtgtttcc agtatcggct ttctcggttt cctttttggt ccgccgctga
     4381 tcggctatat cgcacaactg ttcagcctgc gtacctcctt tggcgtagtg gccattttcg
     4441 ggttattgat tacgttgatg gtcaccaaac tgaaagtaat ggatgatgta aaattgataa
     4501 aaactgcata aatatgaaac tcgatctcgc cgctttccgc cctatcaatg tacaaagagc
     4561 taccgaagga tttaaatgtt atgacaacca gccgctcacc tactggacca ctgccctcgc
     4621 cggtgaagta ggcgaactct gcaatatgat caaaaaaatg cagcgcgtgg aaagaggcgg
     4681 cgtggatggc ggcagcagct atactgctaa agatatcacc aaagaaatgc tcaaagaaga
     4741 aatagggggc atcgcgatct acctggacct gctggcgtcg ctgctggaca tctctttaga
     4801 agaagccatc gttgatacgt ttaacagtaa gtcggagaag tatggattca aggaaaagat
     4861 agtagttagt agttagtagt caacagcccc tcctgagaaa ttttatctta atcaacgaat
     4921 tgcattggca tttcgagcgc
//
LOCUS       DAOPMW010000247         4890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20116_length_4890_cov_9.717890, whole genome shotgun sequence.
ACCESSION   DAOPMW010000247 DAOPMW010000000
VERSION     DAOPMW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20116_length_4890_cov_9.717890"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLU11_11115"
     CDS             complement(<1..526)
                     /locus_tag="PLU11_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59718.1"
                     /translation="MTPKNNIAQELRELESSLADLSAQNTYSVPEGYFEGLAKQVLNR
                     IRAMEAATAAEELGHLSPLLSQLPKTMPYTVPAGYFEEMESTLVSVAMYGEQDASEEL
                     GNISPLLSGLKKEMPYSVPSGYFDSISAPVAETHQPAKVVSMGGSRKWFRYAAAAVVT
                     GVVALLSILYISQDN"
     gene            complement(562..1113)
                     /locus_tag="PLU11_11120"
     CDS             complement(562..1113)
                     /locus_tag="PLU11_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN59719.1"
                     /translation="MSTIASDSELLVQFRVPATKERAYTSLIRKYQEKLYWHIRRMVV
                     EHEDANDVLQNVLIRVWNGLENFREDSQLYTWLYRIATNECLTYLEQQKKKASVSLSD
                     VESGLSNKIVADKHFDANKVEWKLQIAMQHLPEKQRVVFALRYYDEMPYEEMSRVLET
                     SEGALKASYHHAVKKIEDYMLNH"
     gene            complement(1248..2207)
                     /locus_tag="PLU11_11125"
     CDS             complement(1248..2207)
                     /locus_tag="PLU11_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPN59720.1"
                     /translation="MSLKEIQSTGNKDTRSGFGDGIVEAARKNPNVVALTADLLGSMK
                     LNQFIKEFPDRFIQCGIAEANMIGVAAGLTIGGKIPYTTTFANFSTGRVYDQIRQSVA
                     YSGKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVIVPCDYAQTKAATMAVA
                     DYEGPVYLRFGRPVWPIFTADKPFEIGKAQQFSEGTDVTIFACGHLVWNAIQAGIQLG
                     EKGLSVEVINIHTIKPLDEEAVLKSIRKTKCAVTAEEHNIIGGLGDAISQVAAKNFPI
                     PIEYVGTKDTFGESGTPAQLLKKYGLDVPDIVAAAEKVISRKS"
     gene            complement(2279..3334)
                     /gene="galE"
                     /locus_tag="PLU11_11130"
     CDS             complement(2279..3334)
                     /gene="galE"
                     /locus_tag="PLU11_11130"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPN59721.1"
                     /translation="MAKILVTGGCGFIGSHTIVDLLENGYEVISVDNNSRSNPNILLG
                     VEKITGKKVKNYKVDLCNFDDTFAIFQENTDITGIIHFAAYKAVGESVAQPLMYFENN
                     LVSLINLLKCVQEFQIPHFVFSSSCTVYGEPDEIPVTEKTSPKPAESPYGYTKQMSEQ
                     IINEFAKAAKSKNILLRYFNPAGAHPSGIIGEMPLGKPQNLVPAITQTAIGRIEKMHV
                     FGNDYPTRDGSNLRDYIHVCDLAHAHTLSLQYLESGKQQELCKVFNLGTGSGITVLEA
                     IQAFEKTSGTKLNYEIAPRRPGDVVAIYANNDLAREELGWNPKYTLEDIMSTAWKWEL
                     RLKADETVFSSQYGELN"
     gene            complement(3397..4608)
                     /locus_tag="PLU11_11135"
     CDS             complement(3397..4608)
                     /locus_tag="PLU11_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPN59722.1"
                     /translation="MTSFLITIIVLALIFDYINGFHDAANSIATVVSTKVLTPFQAVL
                     WAAVFNSLAFFIFKDHGVADTVAKTVKPDHILQGEMMRVIFSGLIAAIAWNLLTWWLG
                     IPSSSSHTLIGGFAGAAIAAGGFSAVNSPIILKTASFILFAPLMGMLMAFIMSLWFLS
                     SFKKGWTSKIFSVGVIIFVLAFLSLSLEFDRAVLAKKTHYESYINQVIFYSKNFKWIL
                     LSFILLTMATFSLYLSKLNAYRANQWFKRLQLISSAAFSIGHGGNDAQKVMGIITAAL
                     IAGGNIADFKDMPPWVPIACYAAIGLGTMSGGWKIVKTMGTKITKVTPFEGVAAETAG
                     ALTLFITEALKIPVSTTHTITGAIMGVGATKRLSAVRWGVTINLIWAWVLTIPISGLL
                     AGGVYFIVKLF"
     gene            complement(4639..>4890)
                     /locus_tag="PLU11_11140"
     CDS             complement(4639..>4890)
                     /locus_tag="PLU11_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HPN59723.1"
                     /translation="KIAVTELRDMKNMRKITEALVKINSLENQADDVFDMSIERLFAT
                     EPDAKEVIKKREIYQVMEIVTDKCEDASNVIESIIIKYA"
BASE COUNT         1212 a         1178 c         1168 g         1332 t
ORIGIN      
        1 tattatcctg gctgatgtat aatatgctca gtaaagcaac cactcccgtt accaccgccg
       61 ctgccgcata ccggaaccat ttacggctgc cacccatgga taccactttg gctggttgat
      121 gagtttcggc taccggcgca ctgatcgaat caaaataacc ggaaggcaca ctgtaaggca
      181 tttctttttt cagtccgctg agcaggggag atatgtttcc cagttcttca gacgcatcct
      241 gttcaccata catggccacc gataccaggg tggattccat ttcctcaaaa taacccgccg
      301 gtaccgtata aggcatcgtc ttcggcaact gactgagcag gggtgataaa tgccccagtt
      361 cttccgcagc cgtagcggcc tccatcgcac ggatccggtt cagcacctgc ttggccagtc
      421 cttcaaaata accctccggt acggagtagg tattttgggc agacaggtca gccagggagc
      481 tttctaattc cctcagttcc tgtgctatgt tatttttcgg tgtcatttac gttaattagg
      541 acggctaagt aaacccaagg tttaatgatt cagcatgtaa tcctcaattt ttttgaccgc
      601 gtggtggtaa ctggccttca atgccccctc actggtctcc agtacccggc tcatttcttc
      661 ataaggcatt tcatcataat accggagtgc aaatacaact ctttgttttt caggtagatg
      721 ctgcatggcg atctgcaatt tccattctac tttattggca tcaaaatgct tatcagccac
      781 tattttgtta ctcaatccgc tctctacatc gctcaaactc accgaggctt tctttttctg
      841 ctgttccagg taggtcaggc actcattggt ggcaatccgg tacagccagg tgtagagctg
      901 actgtcttcc cggaaattct ccaatccgtt ccatacccgg atcaatacat tctgcagcac
      961 atcattggcg tcttcgtgct cgaccaccat cctccggata tgccagtata gtttttcctg
     1021 gtacttacgg atcagactcg tgtaggccct ttctttggtg gccggtacac ggaactgaac
     1081 cagtaattcg ctgtcggatg caatggttga catggtttta tcgggctttg gtggggctta
     1141 gaatactgga ataaccaatt gtttaattgg cttcacgtaa taaaaataaa aaagcaggtc
     1201 cgattttgaa agaacctgct gaattagttt caaagaaaat agtggaatca gcttttacgg
     1261 ctgattactt tttcggctgc agccacgata tcaggtacat ccaggccata tttcttcaat
     1321 aactgggcgg gtgtaccgct ttcgccaaac gtatctttcg tacctacata ttcgatggga
     1381 atggggaaat tctttgcggc cacctgactg atggcatcac ccagtccgcc gataatatta
     1441 tgttcttccg ccgttaccgc acatttcgtt ttgcggatgg atttcagtac agcttcctca
     1501 tccaggggtt tgatcgtgtg gatattgatt acttccacgc tcagtccttt ttcccccagc
     1561 tggataccgg cctgtatggc attccatacc aggtgaccgc aggcaaagat ggtgacatcg
     1621 gttccttcgg agaattgctg ggctttcccg atctcaaagg gtttatcagc ggtaaaaatg
     1681 ggccataccg ggcgaccgaa acgcaggtaa accggacctt cataatccgc aaccgccatg
     1741 gtggctgctt tggtctgagc ataatcgcag ggtacaatca cggtcatacc gggcagcatt
     1801 ttcatcagtc cgatatcttc gaggatctgg tgcgtggctc catcttcacc cagggtaagt
     1861 ccggcgtggg aggcacagat cttgacattt ttgccgctat aagctacaga ctggcggatc
     1921 tggtcataaa cccgtccggt agagaagttg gcaaaagtgg tggtataagg aattttacca
     1981 ccaatggtca gaccggcagc taccccgatc atattggctt cggcaatacc gcactgaata
     2041 aagcggtccg ggaattcttt aataaactga ttcagtttca tggaccccag caggtcggcc
     2101 gtcagggcca ccacattcgg gttcttacgg gcggcttcca caataccatc tccaaaacca
     2161 ctgcgggtgt ctttattacc ggtggactga atctccttta atgacatgat tatgcttttt
     2221 tgtgccgcaa aagtaaggcg cttgtgccaa tgggcaatga ttaaaaagat ccccgggctt
     2281 aattcaattc cccgtactgg ctgctgaaaa cggtttcatc ggccttaagc cgcagttccc
     2341 atttccaggc ggtggacata atatcttcca gggtgtattt ggggttccag cccagttctt
     2401 cacgggccag gtcgttattg gcgtagatcg ccaccacatc ccccgggcga cggggggcaa
     2461 tttcgtaatt cagttttgta ccgcttgttt tttcaaaagc ctggatggcc tccaacacgg
     2521 tgatcccgct gccggtaccc agattgaata ctttacagag ttcctgttgt ttgccacttt
     2581 ccaggtattg caatgaaagc gtatgggcat gggccaggtc acaaacatgg atataatccc
     2641 gcaggttgga gccgtcgcgg gtgggatagt catttccaaa tacatgcatt ttctcaatac
     2701 ggccgatagc cgtctgtgta atggccggta ccaggttctg gggtttgccc aggggcattt
     2761 caccaataat gccggaggga tgtgcaccag ccgggttgaa ataacggagc aggatgtttt
     2821 tggacttggc agctttggca aattcattaa taatctgctc gctcatctgc ttggtatatc
     2881 cgtaagggga ttctgctggc ttgggactgg ttttttcggt aaccggtatt tcgtcaggtt
     2941 ctccgtacac ggtgcaggaa gaagagaata caaaatgagg aatctggaat tcctgcacac
     3001 atttcagcag gttgatcagg gataccaggt tattctcaaa atacatcaag ggttgggcta
     3061 cggattcacc caccgctttg taagctgcaa aatgaatgat accggtaatg tcagtattct
     3121 cctggaagat cgcgaacgta tcgtcgaaat tgcagagatc gactttatag ttcttcactt
     3181 ttttacccgt gattttttct acgcccaaca ggatgttggg attagaacgg gaattgttat
     3241 ccactgaaat tacttcatag ccgttttcca ggaggtccac aatggtatgt gaaccaataa
     3301 agccgcagcc gcctgttaca agtatttttg ccatataaat taaaatcccg acaaaagtca
     3361 ggattttaat tggtaaaaat actttcggga gtcaggtcag aataatttca caataaagta
     3421 cacaccgccg gccagtaatc cgctgatcgg gatggtcagc acccaggccc agatcaggtt
     3481 gatcgtcact ccccagcgta cggcggataa acgtttggtg gcgccgactc ccatgattgc
     3541 cccggtaata gtatgggtgg tactaacggg gatcttcagg gcttcggtga taaataaggt
     3601 aagggcaccg gcggtttcag cggccactcc ttcaaaaggt gtcactttcg tgatcttggt
     3661 acccatggtt tttacaatct tccatccacc actcatggta cccagaccaa tagcggcata
     3721 acaggcaatg ggtacccatg gtggcatgtc tttaaaatca gcaatatttc caccggctat
     3781 cagggctgct gttatgatcc ccattacttt ctgtgcatca ttaccaccgt gtccgatact
     3841 gaaagcggcc gaggaaatca gctgtaaacg cttgaaccat tggttggctc tataggcatt
     3901 cagtttactc aggtacaggg agaaagtggc catggtgagc aggataaagc tcagcaggat
     3961 ccatttgaaa ttcttggagt agaaaataac ctggttgata taactctcat aatgtgtttt
     4021 cttggctaat acggcccggt cgaattcgag gctaagtgac aggaaggcta aaacgaagat
     4081 gatcacgcca acggagaata ttttggaagt ccatcctttt ttaaaggaac ttaaaaacca
     4141 gagtgacata ataaaggcca tcagcatacc catgagtggt gcaaacagga taaaactggc
     4201 ggtcttcaga attatcgggg agtttacggc actgaatccg ccggcagcaa ttgccgctcc
     4261 ggcaaatccg ccaatcaagg tatgggaaga ggaggaggga atacccagcc accaggtcag
     4321 caggttccag gcaatagcgg caatcagtcc ggagaaaatc acccgcatca tctccccttg
     4381 caggatatga tccggtttta ccgttttagc gactgtgtcc gctacaccat ggtctttgaa
     4441 aatgaaaaag gcaagcgagt taaaaaccgc tgcccaaagt accgcctgaa agggagtcag
     4501 tactttggtg gataccacgg tggcaatgga attcgccgca tcatggaatc cgttgatata
     4561 atcaaagatc agcgccagga cgatgatcgt tatcaggaat gaagtcataa ttgttaatca
     4621 ggtgtggagg aatatcgttt aggcgtactt gataatgatg gattcgatta cattgctcgc
     4681 atcctcgcat ttatcggtaa cgatctccat tacctggtag atctctcttt tcttgatcac
     4741 ttctttggca tcgggttcgg tggcaaagag gcgttcgata ctcatgtcaa acacatcgtc
     4801 ggcctggttt tccaggctgt tgatcttcac cagggcttca gtgatcttgc gcatattttt
     4861 catatcacgc agttcagtta ctgcaatttt
//
LOCUS       DAOPMW010000248         4863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20276_length_4863_cov_7.373128, whole genome shotgun sequence.
ACCESSION   DAOPMW010000248 DAOPMW010000000
VERSION     DAOPMW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_4863_cov_7.373128"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..627
                     /locus_tag="PLU11_11145"
     CDS             262..627
                     /locus_tag="PLU11_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN59724.1"
                     /translation="MTENNRKFDLEDRTSRFAERVRDFCLKLPKNQANNEFVPQLIRS
                     GSSPGANYIEANEGIGGKDFIMKIKTCRREAKESGYWLKLVITDGSSKEEEERSALRQ
                     EAREFVLIFTAILKKKGDS"
     gene            817..2133
                     /locus_tag="PLU11_11150"
     CDS             817..2133
                     /locus_tag="PLU11_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN59725.1"
                     /translation="MQIDHTSFNDISIFHPEEEFSIFHKLNFTRTVGGREWLRKFFTE
                     PHSDLNKIRGTQKVIRTFMEHIHHWPSEISNGTVLVIDKFLDYPIDPISEAPASFNNL
                     LYKWLHNADYSMIKYSMPHFGDFFRGLRQVSELLTDLDLPPAIKLYVDRISGLLKEPP
                     LKKLAETARGERLSTGQNLYFAYHMRGRYKNSTLELIDIFSRLDAWYSMAVAVKTYQL
                     SFPEFVEQRTPLVDAKGLYHILLPTPVAYDLQMNPEQNFVFLTGANMAGKSTLIKSVG
                     AAVFLAHIGMGVPAQHMKLTLFDGLLSNINVVDNIAKGESFFFNEVQRIKNTIDKINN
                     GKKWLVLIDELFKGTNVQDAMKCSLTVIKGLIKIKNSLFILSTHLYEIGEELKTYPNI
                     SFRYFETNVTEDQLSFSYQLREGISNDRIGYVILKREKVVEMLENL"
     gene            2193..3812
                     /locus_tag="PLU11_11155"
     CDS             2193..3812
                     /locus_tag="PLU11_11155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN59726.1"
                     /translation="MKLSLTALFALLFSASVTAQTNTDILHYRFAIELNDQNDTIVGH
                     TQITLVTTSAGRSVTLDLQSISPKGKGMIVGRTVFPYVVNGNDNAPTSRFTHQKNKLT
                     IEALQPFNTGDTLQLFIQYKGIPEDGMIISKNSYGHRTFFADNWPDRAHHWIPCVDQP
                     GDKASFEFLVTAPEHYQVVSNGIQIEETSLSVHKKLTHWREDIALPTKVMVIGVAAFA
                     VQHTGFVNNCIPVSSWVFPENAEKGFYDYSIGTEVLAFFDQYIGPYPYRKLANVQSKT
                     VYGGMENAGAIFYYENSVTGQRSEEGLFAHEIVHQWFGDMATEKSFAHLWLSEGFATY
                     LTHIYMEHQYGTDSLNKRMKEDRASVLSFVKESKRPVVDSTPNYKSLLNANSYQKGSW
                     VLHMLRREIGDSLFHAGVRAYYDLYKGRNAETKDLQVVFERISGKPLETFFRQWLFRG
                     VNPKLQISWKEDPSTDGLIITIDQVQKGDPFEMALEMELHYEGNSKEVRRFDLSKISH
                     SFLITGKGNLLKIIPDPGTSLLAEFNILSGK"
     gene            3884..>4863
                     /locus_tag="PLU11_11160"
     CDS             3884..>4863
                     /locus_tag="PLU11_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPN59727.1"
                     /translation="MLIKTFGSAVYGVEATTITIEVNWIQNQGKDPLIVGLPDNAVKE
                     SLQRIESTFKTNSYDFPRTKVVVNMAPADIRKSGTAFDLPIALGILAASEQISNPESL
                     SSYIIMGELSLNGEIRPIKGALPIAIQARKEGFKGLIVPNVNAKEAGMVNQLQVYGIG
                     HINEVIDFFINGEQGLEPIVINTREEFFHSQYDFEIDFSEVKGQENIKRALEIAAAGG
                     HNAILIGPPGAGKTMLAKRLPTILPPLTLQEALETTKIHSVAGKLPENATLVSKRPFR
                     SPHHTISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQP"
BASE COUNT         1428 a         1048 c         1155 g         1232 t
ORIGIN      
        1 accaagatgc cgaaagaaga accaagaacc aaagatgcca agatgccaaa gaaggaccaa
       61 atgacaagat gccaaaaaaa gatccaagaa ccgaagatgc caagaagcca aagaaggacc
      121 aaatgacaag gatcaaaaaa gcatcaatag ggagaggttg aaagaggaaa agacaagatg
      181 aagaggggga aaaaagcaga aaattgtggt gttttggaca tctcgatgaa aatagttgtt
      241 attaggtttg gttaaaaacc aatgacagaa aataatagaa aatttgatct ggaggataga
      301 accagtcggt ttgcggaacg tgtgagggat ttctgtttaa agttgccaaa aaaccaggca
      361 aataatgagt ttgtaccaca attgatacgt tcaggaagtt cgccaggtgc gaattatatt
      421 gaagcaaacg agggaattgg tggaaaggac tttattatga aaataaaaac ctgccgtcgt
      481 gaagcaaaag aatcaggata ttggttaaag ttggtcatta cggatggcag tagtaaggag
      541 gaagaagaaa gatcagcact cagacaggag gcaagagaat ttgtattaat ttttaccgcc
      601 attctgaaaa agaaaggcga ttcctgaccc agcaacactt ttttgtattt tagttcatag
      661 aaaacataaa tcccaaaact gaagtaaaga acatagaata tcttgtttat tatttgtcct
      721 ttgtaatttg aaccttcttt ggcatcttgg catctttggt tcttggttct tctttcggca
      781 tcttggcatc ttcggttctt ggttcttttt taagttatgc aaatcgacca cacttccttc
      841 aacgacatct ccatcttcca cccggaagag gaattctcca tattccataa actgaacttt
      901 acccgtacgg tgggtggacg ggaatggttg cggaagtttt ttacggagcc gcacagtgac
      961 ctgaataaga tcaggggtac ccagaaagtg atccggacct ttatggaaca tattcatcac
     1021 tggccgtcgg agatcagcaa tggtacggtg ctggttatcg ataaatttct cgactacccg
     1081 atcgatccga tcagtgaggc gcccgcttct tttaataacc tgttgtacaa atggctgcac
     1141 aatgcggatt attcgatgat caaatattcg atgccgcatt tcggggattt tttccgggga
     1201 ctccgacagg tgtccgaatt gctgacggac ctcgacctgc ctcccgccat taaattatat
     1261 gtggaccgaa tcagcggttt actcaaggaa cctcctttga aaaaactggc tgaaacggcc
     1321 aggggggaac ggctcagtac aggtcagaat ttgtatttcg cgtatcatat gcgtggccgg
     1381 tataaaaaca gtacgctgga actgattgat attttcagcc ggctggacgc ctggtattcg
     1441 atggcggtgg cagttaaaac ataccagctc agcttcccgg aatttgtaga acaacgcacg
     1501 cccctggtgg atgccaaagg actgtaccat attctgcttc ccacgcctgt cgcttacgac
     1561 ctgcaaatga atcccgaaca gaatttcgtt ttcctgaccg gggcgaatat ggcggggaaa
     1621 agtacactga tcaaatctgt aggtgccgcc gtattcctgg cccatatcgg aatgggtgta
     1681 cccgcccaac acatgaagct gaccctgttt gacgggctgc tcagtaatat caatgtagtg
     1741 gataatattg ccaaagggga aagttttttc ttcaatgaag tgcaacggat caagaacacc
     1801 attgacaaaa taaataacgg aaaaaaatgg ctggtgctga tcgatgaact gttcaaagga
     1861 accaatgtac aggatgccat gaagtgttcc ctcaccgtga tcaaaggact gatcaaaata
     1921 aaaaattccc ttttcatcct ttccacccac ctctatgaga tcggggaaga gctgaaaacc
     1981 tatcccaata tttccttccg gtattttgaa accaacgtta ccgaagacca gctttccttc
     2041 agctaccagc tccgcgaagg catcagcaat gaccggatcg gctatgtgat cctgaagcgg
     2101 gaaaaagtgg tggaaatgct ggagaatcta taggcaaccg ttcctcattt tccattatga
     2161 cccggcggat tatttttcta tcttgtgtcc ctatgaagtt atccctgacg gccctgtttg
     2221 ccttgctgtt ttccgcgtcg gttaccgccc agacaaatac agatatcctg cattaccggt
     2281 ttgctattga gctgaatgac cagaatgaca ctattgttgg ccatacccag atcaccctgg
     2341 taacaacatc tgcgggccgt tcagtaaccc tcgacctgca aagcatttct ccaaaaggaa
     2401 aagggatgat cgtcggcagg acggtatttc cttatgttgt aaacggaaac gacaatgcgc
     2461 ccaccagccg gttcacacat cagaaaaata aactgaccat tgaagcactc cagccattca
     2521 ataccggtga tacgcttcag ttgtttattc aatacaaagg gatcccggaa gatggaatga
     2581 tcatttcaaa aaacagttat ggccaccgta cattttttgc agacaactgg cctgaccggg
     2641 cccaccactg gattccctgt gtggatcagc cgggtgataa agccagtttt gaatttttag
     2701 tcaccgcccc tgaacactac caggtggtgt ccaatggaat acaaattgaa gaaaccagtt
     2761 tgtccgttca taaaaagcta acacactggc gtgaagacat tgccttgcca acaaaagtga
     2821 tggtgatcgg ggtagcagcc tttgccgtgc agcataccgg ttttgtcaat aattgtattc
     2881 cggtcagttc ctgggtattc ccggaaaatg cagagaaggg tttttatgat tacagcatcg
     2941 gcacggaagt actcgccttc tttgatcagt acatcggccc ctacccttac cgaaaactgg
     3001 ccaatgtaca atccaaaacc gtctatggag gaatggaaaa tgcgggagcc atattttatt
     3061 atgaaaactc cgtaaccggg cagcggagcg aagaaggatt atttgcccat gaaatcgtac
     3121 accagtggtt tggggatatg gctacggaga aaagttttgc gcatctctgg ctcagtgagg
     3181 gatttgctac ctacctgacc catatttata tggaacacca atacggtact gacagtctga
     3241 acaaaagaat gaaagaggat cgggcttcgg tattaagttt tgttaaagaa agtaagcgtc
     3301 cggtggtcga cagcaccccc aactataaaa gtctgctcaa tgcaaacagc taccaaaaag
     3361 gcagttgggt gttgcatatg ttgcgacggg aaatcgggga ctccctattt catgctgggg
     3421 ttcgtgcgta ctatgatttg tataaaggac gtaatgcaga aaccaaagac ctgcaggttg
     3481 tatttgaaag aatttcaggc aaaccgctgg aaacattttt ccggcaatgg ttattccgcg
     3541 gtgtaaatcc gaaattgcag atcagctgga aggaagatcc gtcgacggat ggcctgatca
     3601 tcaccattga ccaggtgcaa aaaggagatc cttttgaaat ggcgctggaa atggaattgc
     3661 attatgaagg gaatagtaaa gaagtacgtc gttttgatct cagtaaaatc tcccatagct
     3721 ttttaataac cggtaaagga aatttgttga aaataatccc ggatccaggg acttccttac
     3781 ttgctgaatt caacatttta tccgggaaat aaaaccattt ttccgggaaa aacacaggta
     3841 gcagaacgga cagtctggct ttattattct ctacctttcc ttcatgctga ttaaaacatt
     3901 tggcagtgcc gtttatggcg tggaagccac cacgattacc attgaagtaa actggattca
     3961 gaaccagggc aaggacccgt tgattgtggg tttaccggat aatgcggtaa aggaaagcct
     4021 gcaacgtatc gagagtacct ttaaaacaaa cagctatgat tttccccgca ccaaagtggt
     4081 ggtgaatatg gcaccggcgg atatccgtaa aagtggtacg gcgtttgacc ttcctattgc
     4141 attgggaata ctcgctgctt ctgaacaaat cagtaatcct gagtccctgt cttcctatat
     4201 tatcatgggt gagttatcac ttaatggtga aatacggccc atcaagggag cattacccat
     4261 tgccatccag gcgcgcaaag aagggtttaa aggattaatc gtgcccaatg taaatgccaa
     4321 agaagccggt atggtcaacc agttgcaggt ctacggcatt ggtcatatca atgaagtgat
     4381 tgattttttt atcaatgggg agcaggggct ggaacccatc gtgatcaata cccgggaaga
     4441 attttttcat tcacagtatg attttgagat tgatttctcc gaagtaaaag ggcaggaaaa
     4501 catcaagcgt gcattggaaa ttgcggccgc cggtggacat aacgccattc tgatcggtcc
     4561 gcccggtgcc ggtaaaacca tgctggctaa aagactgccc accattctgc ctccgctgac
     4621 cttgcaggaa gcattggaga ccaccaagat tcattcggtg gccggaaagt taccggagaa
     4681 tgccacgctg gtttcgaagc gccccttcag aagtcctcat cacaccattt ccgatgtagc
     4741 ccttgtaggc ggaggaggca ttccacagcc cggagaaatt tcactggcac ataacggggt
     4801 gttgtttttg gatgagttgc ctgaatttaa acggacagtt ctggaagtga tgcggcagcc
     4861 gat
//
LOCUS       DAOPMW010000249         4862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20282_length_4862_cov_7.131475, whole genome shotgun sequence.
ACCESSION   DAOPMW010000249 DAOPMW010000000
VERSION     DAOPMW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20282_length_4862_cov_7.131475"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="PLU11_11165"
     CDS             complement(<1..1922)
                     /locus_tag="PLU11_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPN59728.1"
                     /translation="MKRLLLLLAPIFVFMLAFRPVNNVTITGKVTDEKGNPVVGAVIR
                     LKGTHIGTTTGKDGVYTLSVNELKGTLIFSSIDMEQIEVAINGHAVIDVVLNSMQRTM
                     QEVIVTSTHTTKKRMTTSTSIVDGGSSKGIPVNLNNALSGKVAGIQITNGNNNGYYSP
                     APYGYFDGESYEKINENKFLSVSNEPLSTFSIDVDAAGYSNVRRFLNNGQLPPANAVR
                     IEEMVNYFKYEYPQPAGKDPFSINTEISDAPWNKDHKLVMIGLQGKKIPMENLPASNL
                     VFLIDVSGSMQGPERLGLVKASMKMLVEQLREQDKVAIVVYAGAAGLVLPSTSGEEKE
                     TILEAIEKLEAGGSTAGGAGIKLAYKTARNYFIKGGNNRVILCTDGDFNVGASSDQDM
                     ETLIEQERKSGVFLTVLGYGMGNYQDAKMQKLADKGNGNHAYIDGITEAKKVLVNEFG
                     GTLFTIAKDVKLQIEFNPAKVQAYRLIGYENRMLAKEDFNNDKKDAGELGSGHTVTAL
                     YEVIPVGVKSSFFGSVDPLKYQAPKEESTKNVVTDEILTVKFRYKAPDGEVSKLIEHP
                     VLDQSVPIAKTSENFRFAASVAQFGLLLRNSEFKSAASFDNVLNLARKAKNHDEEGYR
                     AEFVRLVESARLLSKG"
     gene            2008..2598
                     /locus_tag="PLU11_11170"
     CDS             2008..2598
                     /locus_tag="PLU11_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN59729.1"
                     /translation="MAFLKQIPQEPLSDKELVTRYKQGGDMAVLGELYQRYMELVYGV
                     CLKYFKEPERAKDSVMAIFEELVSKLKKHEVENFRAWLHQLAKNHCLMALRTPKNMKM
                     VEFKGELMQSEENLHLNGILEKEENFRKLEDCLGSLAAEQRDSIRLFYLEGKSYNEVA
                     SQTGLEWNKVRSLIQNGRRNLKNCMETTGNNEMDFD"
     gene            2626..4044
                     /locus_tag="PLU11_11175"
     CDS             2626..4044
                     /locus_tag="PLU11_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN59730.1"
                     /translation="MANGHTIKKYSATDIEKYQQGLLSPAEAHAMEKAALEDPFLADA
                     MEGYAEAGPTLQTDLNDLKNRLAQRTEEKTKVIPLGGAGKSSFPFMRAAVMIGVLAGA
                     GTLAYLFMFKEKEQPIAQAKPEKNSPAVVATDTGKINANPALTVNDSTIQTGSNNAVL
                     NTEKQTNTFYFNQDSIPAKDEKKLADKEGSDAALASEELVVVNDELKDLAKSTRWKTA
                     DQEKKKAESAKKENGSGIIAIPAPGKTAPAASDQVKAKAVSPAVANKTEESNFLKTNI
                     FRGRVTDGMNFGVPFAKVTNTDDNVGTYTDARGFFNLTSPDSVLHVQVRSIGFENNAS
                     QLRYSVPNNQVILQNDSRSLNALVINNQRANSFRRSDNNNTRLEEPVPADGWENYDTY
                     LSNNLVMPEEYRNKPSTTTTAVEVSFEVDAKGEPVNIRVEKSLCDKCDKEAIRLIKEG
                     PKWKQNARNNRTTVTIPFNQLF"
     gene            4106..>4862
                     /locus_tag="PLU11_11180"
     CDS             4106..>4862
                     /locus_tag="PLU11_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN59731.1"
                     /translation="MKKVFFTLLAALCLINAEAQLKTPAPSPTQTVKQDFGLGSIELS
                     YSRPVMKGRKIFGDLVPFGNVWRTGANSATLVTFSDEVTIGGKKVPAGKYGLLSIPNK
                     DSWTLIISKQLDVTSPAAYKAESDVVRLDVNTMKLAEKTESFTIQFANVKATSCELHI
                     MWENTAVSLPISTDVDAKVMTQIDQLMNKDTRPYFNAAMYYMDNGKDLNQALSWFDKA
                     VEMNGAAFWIHHQRANCLAKLGKKAEAKAAAEKS"
BASE COUNT         1388 a         1219 c         1055 g         1200 t
ORIGIN      
        1 ttccccttac tcagcaaacg ggcactctcc accaggcgga caaactccgc acggtaacct
       61 tcttcatcat gattttttgc tttgcgggcc aggttgagca cattatcaaa actggcggct
      121 gatttgaact cagaattgcg caagaggaga ccaaactggg cgactgaagc tgcgaacctg
      181 aagttttcag atgtttttgc gattggaaca gactgatcca gtaccggatg ttcgatcagc
      241 ttgctgacct ctccatccgg ggctttatag cggaatttca cagtgagtat ttcatccgta
      301 accacattct tcgttgactc ttctttcgga gcctggtact tcaacggatc aactgatcca
      361 aagaaagaac tcttcacacc caccggtatc acttcgtaca aagctgtcac tgtatgacca
      421 ctgcccaatt cgcccgcatc ttttttatca ttattaaaat cttctttggc cagcatccgg
      481 ttctcatacc cgatgagccg gtacgcctgt accttggccg gattgaattc gatctgaagt
      541 tttacatcct tggcaatggt aaataacgtg cctccgaatt cattcaccag cactttcttt
      601 gcttccgtta taccatcgat atacgcatga ttgccatttc ccttatcggc cagcttctgc
      661 atcttggcat cctggtagtt gcccatacca tatcccagta ctgtcaggaa caccccgctt
      721 tttcgttcct gttcaatcag ggtttccata tcctggtcac tgctggctcc tacattaaag
      781 tccccatcag tacaaaggat cacccggtta ttaccgccct taataaagta attacgggcg
      841 gttttatacg ccagttttat accggcacca ccagcagtgg aaccaccggc ttccagcttt
      901 tcaatcgctt ccaggatggt ttccttttct tccccggagg tggaaggcaa aaccagtcct
      961 gcggcaccgg catacaccac gatcgccact ttgtcctgct cccttaattg ctccaccagc
     1021 attttcatag aggcttttac cagacccagc ctttccggtc cctgcattga accagataca
     1081 tcaatcagga aaaccaggtt ggaggcgggc agattttcca tgggtatttt tttaccctgc
     1141 aacccgatca ttaccagttt atgatcctta ttccaaggtg catccgaaat ttcggtgttg
     1201 atggaaaacg gatccttgcc cgctggttgc ggatattcat acttaaaata gttcaccatc
     1261 tcttcgatac gtacagcatt agccggtggt aactggccat tattaagaaa gcggcgtaca
     1321 ttgctgtaac cagccgcatc cacatcaatg gagaaagtgg aaagtggctc attacttaca
     1381 gataaaaatt tattctcgtt gatcttctca tagctttccc catcaaaata accataaggt
     1441 gccggtgaat aatagccatt attgtttcca ttggtgatct gtataccagc caccttacca
     1501 gacaatgcat tattcagatt aaccggaatg cctttactgc ttcctccatc gacgatacta
     1561 gtgcttgtag tcattctctt tttcgttgta tgggtgctgg ttacaatcac ttcctgcata
     1621 gtacgttgca tgctattaag caccacatct attactgcat gaccgtttat tgcaacttcg
     1681 atttgttcca tatcgatcga agaaaaaatc agtgttcctt tcagttcatt aacactcagg
     1741 gtatacacac catcttttcc agtggtagtg ccaatatggg ttcctttcag acgaataaca
     1801 gctccaacaa ccggattacc tttttcatct gttaccttac cggtgattgt gacattatta
     1861 accgggcgaa aagccagcat aaatacaaat attggggcca gcagaagaag tagtcgtttc
     1921 atattttagc attttcatac ccagatgcgg gtattttagc cattgcataa atcgttcata
     1981 aaaattttat tccctaattt taaggccttg gctttcctga agcagatacc acaagaacct
     2041 ctctctgata aagagctcgt tacccgctat aaacaagggg gcgatatggc cgtgctcggc
     2101 gaactctacc aacgctatat ggaactggtc tacggggttt gcctcaaata cttcaaggaa
     2161 cccgaacggg ctaaagacag tgtgatggcg atctttgagg aactggtatc aaaattgaag
     2221 aagcatgaag tggaaaactt ccgggcctgg ctgcaccaac tggctaaaaa tcattgtctc
     2281 atggccctcc gcaccccaaa gaacatgaaa atggtggaat tcaaggggga acttatgcaa
     2341 tcggaggaaa acctgcatct gaatggcata ttggagaagg aagaaaactt ccggaaactg
     2401 gaagactgcc tggggagcct ggcagccgag caacgggatt caatccggct tttttacctg
     2461 gagggcaaat cctacaatga ggtggccagc cagaccgggc tggaatggaa taaagtcagg
     2521 agcctgatcc agaacggaag acggaacctg aaaaactgta tggaaaccac agggaacaac
     2581 gaaatggatt ttgattaaaa aaaattaaga actttatact gccggatggc aaacgggcat
     2641 accataaaaa aatattcagc aactgatatc gaaaaatatc aacagggatt gctttcacca
     2701 gccgaggcgc atgccatgga aaaagccgcc ctggaagacc ccttcctggc cgatgctatg
     2761 gaaggctatg cagaagcagg ccccacgctg cagacagacc tgaatgacct gaagaacaga
     2821 ctggcccaac gcacggagga aaaaacaaaa gtgattcccc tgggaggtgc cggcaagtcc
     2881 tccttcccct ttatgcgggc ggccgtcatg attggggtat tggcaggtgc aggtaccctg
     2941 gcctacctgt ttatgtttaa agaaaaagaa cagccgattg cccaggcaaa accggaaaaa
     3001 aacagcccgg ctgtagtggc aacggatact ggtaaaatca atgccaatcc ggctctaact
     3061 gttaacgatt caacgattca aaccggcagc aacaatgccg tattgaatac agaaaaacag
     3121 acaaacacct tttattttaa ccaggacagt ataccagcaa aagatgaaaa gaaacttgct
     3181 gacaaggagg gttcagacgc tgctctggcc agtgaagaat tagtagtggt caatgacgaa
     3241 ctgaaagacc ttgcaaaatc aacccgttgg aaaactgccg atcaggaaaa aaagaaggcg
     3301 gaatctgcta aaaaggaaaa cggatcaggc attatcgcca ttccggcacc cggtaaaact
     3361 gcaccagcag cgtctgatca ggtaaaagcc aaggcagttt cacctgcggt cgccaataaa
     3421 acagaagaaa gcaattttct taaaaccaat atattccgtg ggcgggtgac tgacggtatg
     3481 aactttggtg ttccttttgc caaagtcacc aataccgatg acaatgtcgg aacgtatacc
     3541 gatgcccgcg ggttcttcaa ccttacctcc cccgactccg tgctgcatgt acaggtcagg
     3601 tccattggtt tcgaaaacaa tgccagccag ttgaggtata gtgtacccaa taaccaggtc
     3661 attctccaga atgacagccg cagtctcaat gccctcgtga tcaataacca gcgtgccaat
     3721 tccttccgcc gttctgataa taataacacc aggctggaag aacccgtacc ggctgatggc
     3781 tgggaaaact atgacaccta ccttagtaat aacctggtga tgccggaaga ataccggaat
     3841 aaacccagta ccacgactac cgctgtcgaa gtatctttcg aagtggatgc caaaggggaa
     3901 ccggtgaata tccgggttga aaagtcgctt tgcgataaat gcgacaaaga agccatccgt
     3961 cttatcaaag aaggcccgaa atggaaacag aatgcccgga acaatcgtac caccgtgacc
     4021 attccgttca accaactgtt ttaatccaca gaccatccgg aggtacttat ttcccttacc
     4081 ttcgtcaatc aaaaatcagc ttattatgaa aaaagtattt tttaccttac tggctgcttt
     4141 atgcctgatt aacgctgaag cccagctaaa gacccccgct cccagtccca cgcaaaccgt
     4201 gaaacaggac tttggcctgg gcagtattga actttcttat tcacgcccag tcatgaaagg
     4261 tcgtaaaata ttcggcgacc tggttccttt tggaaatgtt tggcgtaccg gtgccaatag
     4321 tgccaccctg gttactttca gcgatgaagt aactattggc ggtaaaaaag tacctgccgg
     4381 caaatatgga ctgcttagca tacccaacaa ggacagctgg accctgatca tctccaaaca
     4441 actcgatgta accagtccgg ccgcctacaa agccgaaagc gatgtagtac gtttggatgt
     4501 gaacactatg aaactggcgg agaaaacaga atccttcacc attcagtttg ccaatgtaaa
     4561 agccaccagt tgtgaactgc acattatgtg ggaaaacacc gcagtctccc tgccgatcag
     4621 tacggatgtc gatgctaaag taatgaccca gatcgatcag ctgatgaata aagacacccg
     4681 tccttatttc aatgcggcca tgtactatat ggacaatggc aaagacctca accaggccct
     4741 gagctggttt gacaaagccg ttgaaatgaa tggggctgct ttctggatcc atcatcagcg
     4801 tgccaattgc ctggccaaac tgggcaagaa agccgaagcc aaagccgccg ctgaaaaatc
     4861 ca
//
LOCUS       DAOPMW010000250         4853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20336_length_4853_cov_6.043560, whole genome shotgun sequence.
ACCESSION   DAOPMW010000250 DAOPMW010000000
VERSION     DAOPMW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20336_length_4853_cov_6.043560"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLU11_11185"
     CDS             <1..262
                     /locus_tag="PLU11_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPN59732.1"
                     /translation="FADIGNIWLMKEQESYAGGNFSFNRFYKEFAIDVGLGLRLDFNF
                     FILRVDFATPLRQPAMPAGKRWVANDWHISDVIINFGIGYPF"
     gene            complement(275..1348)
                     /locus_tag="PLU11_11190"
     CDS             complement(275..1348)
                     /locus_tag="PLU11_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPN59733.1"
                     /translation="MKKEDFGHDNRTAFEAKTEAQKIAFGPVMFQAARALIKLGILHE
                     VSEAGKQGVASFEIAEKLGLTIYGTEVLLDAGLSMGIVLLKDEKYILTKTGYFILKDT
                     MTRVNMDFVHDVCYEPFFYLEESVRNGKPEGLKVFGEWKTIYPALQFLPEQAKRSWFA
                     FDHYYSDVAFPEIIPVLAAGKPRRLMDIGGNTGRFAIQCVNQIPGLNVTIVDLPDTLN
                     AAENIITGMGLQDRISFFAADMLDSGQRLPDGFDAIWMSQFLDCFSEEEIISILRRAS
                     TAMNPGALLYILELFWDRQKYEASSYSLNATSLYFTCLANGNSRMYHSADLYRMIEKA
                     GLKIKEVSDHIGISHTLVTCTGS"
     gene            complement(1385..2530)
                     /locus_tag="PLU11_11195"
     CDS             complement(1385..2530)
                     /locus_tag="PLU11_11195"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPN59734.1"
                     /translation="MPVFREMTSNESNRPFSFLVTVLAGASAGLLVRQLLNHRTDYRY
                     TGTVIRTLLVSLILEPFRWWEIIRWRRKVRKVVISKPPVFVIGFWRSGTTLLHNLLCC
                     LPDAAYVTTFQTVFPGLLLSGSWWFKPIIGKFWPTHRPFDQVRMGMHLPQEEEIALAN
                     LQRISFYNFLIFPKDFERFYHRELFFHDIDSETKIKWIHKYKELIRKAMMNTGGDRLI
                     SKSPSNMARIPTLLEMFPGARFIFLYRDPYTTVESFYRFFHEVLPVMQLQDSGNILSR
                     DRMARVYADMVRQYLDDKYLIPKENLVELRYEQFLVNIRQELQEVCQQLGIPGFEEAR
                     PQMEKYLNETAGHRKSGYEIHADTIKYINQYASDIVEFLGYPHRLNE"
     gene            2711..3682
                     /locus_tag="PLU11_11200"
     CDS             2711..3682
                     /locus_tag="PLU11_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HPN59735.1"
                     /translation="MSKVEYDTSYISTYTTDYTCRLFGSVKYGQTSYNDNLNGKSLYY
                     KPNSKFLPGIGINHGFLGLNIAINLPFLNQDDDKYGETKYYDLTARVFSPRFNSTLYL
                     QYYRGFYLSNTRDMVPGWEYGDPYYTRGDIRSVTAGLDLIYIFNSRKFSYRAAVIQNE
                     WQKKSSGSFLGGGSIIYNATFGDSSLVPSNLYYQSFYDGLKIDRSNNFSLGPAFGYAY
                     TVVIKKHYFIMGSLNGSGTIGFTRFIIHENDEKVKSGVVFGLRSEILVSAGYNSEKWY
                     FGLSFVNMSVTNQSPLDECTINYDTGMFRFNLARRIPTKKPIRLLNS"
     gene            3797..4741
                     /locus_tag="PLU11_11205"
     CDS             3797..4741
                     /locus_tag="PLU11_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HPN59736.1"
                     /translation="MKSITAIFRLLTITLPLAVAGQQLENPGFEEWETAAPSIIEPTE
                     WNSIKTADDPGIAAMAPITIDRSEDAHTGNYSLKLYNVTPFPNLVATGAITNGRFHAE
                     FDLTKSYSYTQRSDPKYHTSFTWRPDSLTGWFKYFPNLNDAAQFKVILHVDSCKLPTD
                     SLSQLNIVGMGVFVTEHGVTYDSWTRFSVPFQYFNDSLPEYALTTMNSGDSITAIDGS
                     YLLIDDLELKYAPAGINEPKKPDSFLTYGKGILNLTLAPETYMNQWLYIIDASGQTVY
                     STRLEKGTINLPESIPGAVYIALLITSNRQYAQKILIP"
BASE COUNT         1361 a         1130 c         1072 g         1290 t
ORIGIN      
        1 ttttgcagac attggcaaca tatggctgat gaaggaacag gagtcatatg ccggaggtaa
       61 tttcagtttc aaccggtttt acaaggagtt cgccatagat gtggggcttg ggctgaggct
      121 tgatttcaat tttttcattt tgcgtgtcga ttttgccact ccgcttcgcc agcctgcaat
      181 gccggcaggc aaacgttggg tagccaatga ctggcatatc tccgatgtca tcatcaactt
      241 tgggatcgga tatcccttct gaccgggggt gatgtcagga tccagtgcag gtcacgagtg
      301 tatgggaaat cccgatatgg tccgacactt ctttgatttt caatcctgct ttctcaatca
      361 tccggtataa atcagccgag tggtacatcc ggctgttacc atttgccagg cacgtaaaat
      421 acaatgatgt ggcattgagg ctataggatg aagcctcgta tttctgccga tcccagaaaa
      481 gttccaggat ataaagcaat gcacctggat tcatggctgt agaggccctt ctaagtatac
      541 tgatgatctc ttcctctgaa aagcaatcga ggaactggct catccagatg gcatcaaaac
      601 cgtcgggcaa tcgttggccg gaatcaagca tatcagcggc aaagaagctg atgcggtcct
      661 gcagtcccat tccggtgatg atattttccg cagcgttaag ggtatccggc aaatccacaa
      721 tggttacgtt caaaccgggg atctgattaa cgcattgtat ggcgaaccgg cctgtgttac
      781 ctccgatatc catcaggcgc ctgggtttgc ccgctgccag caccgggata atttcaggaa
      841 aggccacatc cgagtaataa tggtcaaaag caaaccagct ccttttggcc tgctccggaa
      901 ggaactgcag agcgggataa atcgttttcc actctccaaa tacttttaat ccttccggtt
      961 taccgtttcg caccgattcc tccaggtaaa aaaacggttc ataacagaca tcatggacaa
     1021 aatccatatt gacacgtgtc atggtatctt taagaataaa ataaccggtt ttggtcagga
     1081 tgtatttttc atccttcaac aaaacgattc ccatactcag tccggcatcc agcaaaactt
     1141 cagtgccata aatggtcagg ccaagttttt cagctatttc aaaagacgcg acgccctgct
     1201 ttccggcttc gctgacctca tgcaaaatgc caagttttat caatgccctg gctgcctgga
     1261 acataacagg tccgaaagct atcttctgcg cttccgtttt ggcttcgaag gctgtacggt
     1321 tatcatgccc gaaatcttct ttcttcatct tcttttaata aaaaaaccgt tgcgaatata
     1381 agggttattc gttcaaccgg tgaggatagc caagaaattc gactatatcg gaagcgtatt
     1441 ggttaatata ttttatcgta tccgcatgaa tttcgtagcc ggatttccgg tgacctgccg
     1501 tttcattcag gtacttctcc atttggggcc gggcttcctc aaaaccggga ataccaagtt
     1561 gctggcacac ttcctgaagt tcctgccgga tattaaccaa aaattgttca taacgaagct
     1621 ctaccaggtt ttctttggga atcaaatatt tatcatccaa atactgcctg accatgtcgg
     1681 catataccct ggccatccgg tcacgtgaca gaatatttcc ggaatcctgc aattgcataa
     1741 cgggaagcac ttcatggaaa aagcggtaaa aagattcgac cgtggtgtaa ggatcacgat
     1801 aaaggaagat gaacctggct ccgggaaaca tctccagcag cgtcggaatt cgtgccatat
     1861 tggaagggct tttggaaatc aaccggtcac cgccggtatt catcatcgct ttgcggatca
     1921 gttccttgta tttatgaatc catttgatct ttgtctcact gtcgatgtca tgaaaaaaaa
     1981 gttcgcggtg atagaaccgt tcaaagtctt tcggaaaaat aaggaaatta taaaagctta
     2041 ttctttgaag attagccagg gcgatctcct cttcctgcgg caagtgcatt cccatcctga
     2101 cttgatcaaa aggacgatgg gtgggccaga atttcccgat gatcggttta aaccaccagg
     2161 aacctgaaag aaggaggccg ggaaaaaccg tctggaaggt agtcacatat gccgcatccg
     2221 gcaaacagca aaggaggtta tgcagtaaag tagtgccgct ccgccagaaa ccgatgacaa
     2281 aaaccggggg ttttgagatc actacctttc tgacttttct tctccaacgg ataatctccc
     2341 accatcgaaa gggttcaagg atcaaggaaa ccagcagggt gcgaataacg gtgccggtgt
     2401 aacggtaatc ggttctatgg ttaagtaatt gcctgacaag caacccggct gatgcgccgg
     2461 ccagtaccgt caccaggaat gaaaacggcc gattggattc attggaggtc atttccctga
     2521 aaactggcat ccaaacaaaa ataataatcc ttttctaatt tatcaagcga acaggaaaga
     2581 ttacgtattt ttgacactcc cggatttagt ttttataatg ctcaaaaaca agaaatatcc
     2641 aatctgcctt tcacttcttc tatttattcc cggattgagt gttttcggtc agcgcgattt
     2701 tttaaagaat ttgtcgaagg ttgaatacga caccagctat atttcgacct acaccaccga
     2761 ctatacctgc aggctattcg gttcggtcaa atacggacaa accagttaca acgacaatct
     2821 gaacggtaag agtctgtatt acaagcccaa cagcaagttt ctgcccggga ttggcatcaa
     2881 tcatggattt cttggattga acatcgcaat aaacctgcca tttttgaacc aggacgacga
     2941 caaatacggt gaaacaaaat attatgacct cacggcgaga gttttttcac cgcggttcaa
     3001 ttccaccttg taccttcaat attacagggg attttacctg agcaacaccc gggatatggt
     3061 tccgggatgg gagtatggtg atccctatta caccaggggg gatatacggt cggtcacggc
     3121 tgggcttgac ctcatttaca ttttcaacag caggaagttc tcatacaggg cagcggttat
     3181 tcagaatgaa tggcagaaga aaagttcggg atcattcctg ggcggaggca gtatcattta
     3241 taatgccaca tttggtgatt cgtcgctggt accttcaaat ctttattatc agtcatttta
     3301 tgatggcctt aagatcgacc ggagcaacaa tttctcactg ggccccgcat tcggatacgc
     3361 ttatactgtg gtgataaaaa agcattattt catcatggga tcattgaacg gatcgggcac
     3421 tatcgggttc acccgtttca ttattcatga aaacgatgaa aaagtgaaat cgggagtggt
     3481 attcgggctc cggtctgaaa tcctggtatc ggccggttac aacagtgaaa aatggtattt
     3541 cgggctttct ttcgtcaata tgtcggttac caaccaatcg ccactcgacg aatgcacgat
     3601 caattacgat accggaatgt tccggttcaa cctggccaga aggattccca ctaaaaaacc
     3661 gataaggcta ctcaactcgt gaaaaatctg acggttcata aatactcata ctaaatattc
     3721 ataattattt ggatttttat aattttaccc tcccgaaagt caatttttta atgtttaacc
     3781 ataaacaagc ccagatatga aaagcattac agccatattc cggcttttaa ccatcaccct
     3841 gccgctggca gttgcgggac agcaactgga aaatcccgga tttgaggagt gggaaacagc
     3901 agcgcccagt attattgagc ctacagagtg gaacagcatc aagacggccg atgacccggg
     3961 aattgcagcc atggcgccga tcaccatcga ccggagtgaa gatgcccaca ccggtaacta
     4021 ttccctgaaa ctttacaatg tcactccttt cccaaacctg gttgccacgg gagctattac
     4081 caacggaagg tttcacgcgg aattcgatct caccaagagc tattcatata cgcaaaggag
     4141 cgatccgaaa tatcacacca gcttcacctg gcgtccggac agtctgacgg gatggttcaa
     4201 atatttcccc aacctgaacg atgcggccca gttcaaagtg atcctgcacg ttgactcatg
     4261 taaattacca accgactcat tatcacaact caacatcgta ggcatgggcg tttttgtcac
     4321 tgaacacggt gtgacttatg attcttggac ccgcttttcg gtacctttcc aatatttcaa
     4381 tgacagttta cctgaatatg cgctgacgac tatgaattca ggcgacagca ttacagccat
     4441 cgacgggagt tatcttttga tcgacgatct ggaactcaaa tatgctccgg caggtatcaa
     4501 tgaacccaaa aaaccggact catttctcac ctatggcaaa ggtattttaa atctgaccct
     4561 tgccccggaa acttatatga accaatggct ttatattatt gatgcatcgg gacagaccgt
     4621 ttattcgaca cggttggaaa agggtacgat aaaccttcct gaaagcatac ccggtgccgt
     4681 ttatattgca ttactgataa ccagtaacag gcaatatgcc caaaaaatat tgatcccata
     4741 aagaatgaag aaaattgtct tgttattggc cgttttactt acggcggttt atgcattcag
     4801 ccaggataaa ggaacattca gagggattgt cagggataaa acgaacaacg aac
//
LOCUS       DAOPMW010000251         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20375_length_4846_cov_6.037153, whole genome shotgun sequence.
ACCESSION   DAOPMW010000251 DAOPMW010000000
VERSION     DAOPMW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20375_length_4846_cov_6.037153"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLU11_11210"
     CDS             <1..338
                     /locus_tag="PLU11_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPN59737.1"
                     /translation="MANVGQLEMYKLEKNLDILSVISKAAPIFGFVGTLIGLMQLFSG
                     ITSSNKFDVSIISGGIYTKLITSISGLVIGLIAYLCYSYLNTQIDKTANKMEAASADF
                     LDILQEPTR"
     gene            348..749
                     /locus_tag="PLU11_11215"
     CDS             348..749
                     /locus_tag="PLU11_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPN59738.1"
                     /translation="MKFRKKHRKESEVFTDSLNDILFILLMFFLIVATLANPNVVPVG
                     LPRGKKETKAKQDIMVSINKDQEYFIGSTKVDAASFDSLLSLEIRKQKAFVDTPTVVI
                     NADTSAFYGGVFHVMGIAKKDTAKVVARVQQ"
     gene            902..1267
                     /locus_tag="PLU11_11220"
     CDS             902..1267
                     /locus_tag="PLU11_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59739.1"
                     /translation="MKRVAIILFLSVFFFSCKKETNISIVGNWIEDANYYMDSTGQYT
                     WGPSSRWPLNLTFTTDGTCEFFQEMAGSTCSYYYNPSTRELIYDSNAAPAYKVSLLNE
                     EFLILDSHYPGEKTRYRRK"
     gene            complement(1472..2335)
                     /gene="prmC"
                     /locus_tag="PLU11_11225"
     CDS             complement(1472..2335)
                     /gene="prmC"
                     /locus_tag="PLU11_11225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPN59740.1"
                     /translation="MTLQEAGFFLLNKLRTVYDEGEASAIGDWVMESLTGSDKTERMI
                     YKNNPITPEEEEQLHGYAAKLLKHTPVQYVLNESWFCGLKFYVDENVLIPRPETEELV
                     EWVISNCRFPIDTLSILDIGTGSGCIPISLKRRLRKAKVTALDISDGALTVAQRNAAK
                     LGAEVEFIQLDILDPAQWAILKTADIIISNPPYIPENNKASMHPNVLEFEPHNALFVP
                     DQDTLLFYKAIIALAKTKLTLEGLLFFEIHENLGKEVTELLSAAGFMSEIKKDMQGKD
                     RMVMGRKSLEG"
     gene            2467..3552
                     /gene="ribD"
                     /locus_tag="PLU11_11230"
     CDS             2467..3552
                     /gene="ribD"
                     /locus_tag="PLU11_11230"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPN59741.1"
                     /translation="MTTDEKYMYRCIELARKGAGYVAPNPMVGAVLVRDDRIIGEGWH
                     QRYGEAHAEVGAIGEATGLATLSGLTSSFSSNTTLYVSLEPCAHHGKTPPCADLIIRT
                     GIPRVVIGCRDPFEAVNGKGIEKLKAAGIEVVEGLLEKECRELNKRFFTFHEQQRPYV
                     ILKWAQTADGFMAAEAESPRLLITNEYSNRRVHQWRSEEDAIFVGTNTALLDDPELTN
                     RLWTGPSPVRLVLDMELRLPASLKLFSKEQKTIVFNSIKEEEQPNLIYYRLEKNKSLI
                     SQFMDALYQLRIQSVLIEGGRRLLDSFILAGAWDEARIITNMQKKIGNGLAAPELMNK
                     QRINQEKFLSDELLTWINTVQIKTNDI"
     gene            3549..3782
                     /locus_tag="PLU11_11235"
     CDS             3549..3782
                     /locus_tag="PLU11_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPN59742.1"
                     /translation="MNWIDWVGYLAAGLVVISFMMGSNLRTVRLINMFGAITFLIYGV
                     LLDVNLPIIIPNGVITMIQFYYLFIKKEKRDGE"
     gene            3769..4386
                     /locus_tag="PLU11_11240"
     CDS             3769..4386
                     /locus_tag="PLU11_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPN59743.1"
                     /translation="MTENNTETFYYNTVEQAGQAEFKDRGSRFIAYVYPLADINDFKE
                     KLAALKKEHPKAVHHCFAYRLGLDSNTFRVSDDGEPSGTAGKPILGQIDSKKITNVLI
                     VVVRYFGGTLLGVPGLINAYKTVAALALQVIPTVAKPIQLNYHIQFDYTQMNEVMRVI
                     KHFDCTVHRQETQLFCSIDAGIPKNRLNEVLFRLKELRGVDCTVI"
     gene            complement(4383..>4846)
                     /locus_tag="PLU11_11245"
     CDS             complement(4383..>4846)
                     /locus_tag="PLU11_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPN59744.1"
                     /translation="IINHPGTSKEVTDNIVALSKDLGKVPCVVNDFPGFISNRILMPM
                     INEAIYCLHEGVAGVTEIDTIMKLGMAHPMGPLQLADLIGLDVCLSIMNVLLDGFNNP
                     KYTPCPMLVEMVKNGKLGAKSGEGFYVHKAGSKELVVSDLTVQATPSGQNS"
BASE COUNT         1293 a         1087 c         1146 g         1320 t
ORIGIN      
        1 gcatggccaa tgtgggtcag ctggaaatgt acaaactgga gaaaaacctg gatatcctgt
       61 ccgttatttc aaaagcagcc cccatttttg gtttcgttgg taccctgatc ggtctgatgc
      121 agttgttttc aggtatcact tcctccaata agtttgatgt gagtattata tccggtggta
      181 tctataccaa actgattact tccatttccg gactggtgat tggtctgatc gcttacctgt
      241 gctacagtta cctcaatacc cagatcgaca agaccgccaa taaaatggaa gcggcttctg
      301 ctgatttcct ggacatcctg caagaaccca cccgataatt tacctacatg aaattcagaa
      361 agaaacatag aaaagaatcg gaggtcttta cggactcgct gaatgatatt ctcttcatcc
      421 tgctgatgtt ctttttgatc gtggctacac tggcgaaccc caacgtggtt ccggtaggac
      481 taccccgggg taagaaagaa accaaggcca agcaggatat catggtgtcc attaataaag
      541 accaggaata cttcataggc tctactaaag tcgatgccgc cagttttgat tcactcctta
      601 gcctcgaaat caggaaacaa aaagcttttg tggatacgcc aacggtggtg atcaatgccg
      661 ataccagtgc tttttacggg ggggtgtttc atgtcatggg tattgctaag aaagacacgg
      721 cgaaagtggt ggcgagagtg cagcagtaga gaagtcgata gcctgcctgc cggcaaaggc
      781 aggcccatat tgtagctggt tgggatacca gttttgggtg tggaattcag ggtaaatacg
      841 gcgtaatact accacgcatt tcccaggggg gatgactact tttaccgggt caatcctacc
      901 aatgaaacga gttgcaatca tcctcttctt atccgtcttc tttttttcat gtaaaaagga
      961 aaccaatatt tcaattgtcg gcaactggat tgaagacgcg aattattaca tggatagtac
     1021 cggtcaatat acctggggcc catcaagccg ctggccatta aacctgacct ttacaacgga
     1081 tggcacttgc gaatttttcc aggaaatggc tggaagcacc tgttcctact actataatcc
     1141 ttctacaagg gaattaattt atgacagcaa tgctgcaccc gcttacaaag tttccctgct
     1201 gaacgaagaa tttttaattc tggatagtca ttatcccgga gagaaaacga ggtacaggag
     1261 aaagtagggc gtcggtagtc agcagtcggc agtcaacagt cggcagtcgg cagtcagcag
     1321 tcagcagtcg gcagcccatt ctaaaaagga ttgtctaaac cttctaaacc cttcattaaa
     1381 acgctatctc ctttcggcct ttataaaccg tactttacta ctcattctgc tttaatacgc
     1441 tataaactct tctttaaatc gctttcttca gtcagccttc taaactcttt cttcccatta
     1501 ccatccggtc ttttccctgc atgtctttct ttatttctga cataaatcct gctgcggata
     1561 ggagttccgt tacttccttt cccaggtttt catgaatttc aaaaaagagg agaccttcca
     1621 gggtaagttt tgtttttgcc agggcaatga tggctttata aaacaataat gtatcctgat
     1681 ccgggacaaa caaagcgttg tgcggttcga actccaacac attcggatgc atggatgctt
     1741 tattgttttc aggtatgtaa ggcgggttac tgatgatgat atctgccgtt ttcagtatgg
     1801 cccattgagc ggggtccagt atatccaatt ggatgaattc cacttcggct cccagtttgg
     1861 ccgcatttct ttgggccacc gttaaagccc catcactgat atccagggcc gtgacctttg
     1921 cttttctcag tcttcttttt aatgagatgg gaatacagcc actgcctgtg ccaatatcca
     1981 aaatggataa agtgtcaata ggaaacctgc aattggaaat gacccattcc accagttctt
     2041 ctgtctcagg gcgtgggatc aatacgtttt catccacata aaacttcagc ccgcaaaacc
     2101 aggattcatt cagcacatat tgtacgggtg tatgttttaa gagttttgcg gcatatccat
     2161 ggagctgttc ttcctcctcc ggtgtaatgg ggttgttttt gtagatcatc cgttcggtct
     2221 tgtctgaacc ggtcagcgat tccattaccc aatcacctat agcagaagct tctccttcat
     2281 cataaacggt gcggagttta ttcaataaaa aaaagccagc ttcctgtaaa gtcattcgca
     2341 aatatagtta atagtggagt ggtgagtggt gagtggtgag tggtgagtgg tgagtagtga
     2401 gtggctccta acagctaaca actaacagct aacaaccttc tcttatcttt gcgggattaa
     2461 ctgaaaatga ccaccgacga aaaatacatg taccgctgca tcgaactggc ccggaaagga
     2521 gctggttatg tggcgcctaa cccgatggtt ggggcggtgc tggtgcggga cgaccggatc
     2581 atcggagaag gctggcacca gcgttacggg gaagcgcatg cggaggtggg tgctattgga
     2641 gaagctactg gtctggccac tctgagtggc ctgaccagta gcttctcctc caacaccact
     2701 ctctacgtct ccctcgaacc ctgcgcccat cacggaaaga ctcctccctg tgccgatctg
     2761 atcatccgca ccggtattcc ccgggtggtt attggctgcc gggatccttt tgaagcggtg
     2821 aatggtaagg gtattgaaaa attaaaagca gccggtattg aagtggtgga agggttattg
     2881 gaaaaggaat gccgggaact gaataagcgg ttttttacat ttcatgaaca gcaacggccc
     2941 tatgttatcc ttaaatgggc acaaacggct gatggtttta tggccgctga agctgaaagc
     3001 ccaagattat tgatcacgaa tgaatattcc aaccgccgtg tgcaccagtg gcggagtgaa
     3061 gaagatgcta tttttgtcgg taccaatacc gctttgctgg atgacccgga gcttacgaat
     3121 cgtttatgga ccggcccctc accggtgcgg ctggtactgg acatggaact ccgtttgccg
     3181 gcttcgctga aattgtttag caaggaacag aaaaccattg tctttaattc gataaaggaa
     3241 gaggagcagc ccaacctgat ttattaccgg ctggaaaaaa ataaaagcct tatttcgcag
     3301 ttcatggacg ccttgtacca gctcaggata caaagtgtgc tgattgaagg ggggcgccgg
     3361 ctattggaca gtttcatact ggccggggcc tgggatgagg ccagaataat taccaatatg
     3421 caaaagaaaa tcgggaatgg actggctgca ccggagttga tgaacaagca acggattaac
     3481 caggaaaagt ttttatcaga tgaattacta acctggataa atacagtaca gattaaaacc
     3541 aatgatatat gaactggata gattgggtag gatacctggc tgccggactg gtggtgatca
     3601 gcttcatgat gggatccaat ctcaggaccg tgcgactgat caatatgttt ggggcaatca
     3661 cctttttgat ttacggagta ctgctggatg tcaacctgcc gattattata ccaaacgggg
     3721 ttatcactat gatacaattc tattatctct ttattaaaaa ggaaaagcgt gacggagaat
     3781 aatacggaga ccttttacta taataccgtt gagcaggcgg ggcaggcgga atttaaagac
     3841 cgggggagcc gttttattgc ctatgtctat cccctggctg atatcaatga tttcaaggaa
     3901 aaactggctg ctttaaaaaa agaacatccg aaagccgtac atcattgctt tgcttaccgg
     3961 ctaggactcg atagcaacac ctttcgtgtc agtgatgatg gcgaaccttc cggtacggca
     4021 ggtaagccca tcctcgggca gatcgacagt aaaaagatca ccaatgtact gatcgtggtg
     4081 gtacgttatt ttggcggtac gctactcggg gtgcccggac tgataaatgc gtataaaacg
     4141 gtagctgcgc ttgccttaca agtgataccg actgtagcaa aaccgattca gctcaattat
     4201 catattcagt ttgattatac acaaatgaat gaagtgatgc gggtgatcaa gcactttgat
     4261 tgcaccgttc accggcagga gacacagtta ttctgttcca ttgatgcagg catacccaag
     4321 aacaggttga atgaggtatt gttcaggctg aaggagctga gaggggttga ttgtactgtg
     4381 atttaactgt tctggccact aggagtggcc tgaacagtta aatcactcac caccaattcc
     4441 ttactccctg ctttatgcac ataaaatcct tccccgcttt tggctcccag ctttccattc
     4501 ttcaccattt caaccaacat gggacaagga gtatattttg gattgttgaa tccatccagt
     4561 aatacattca taatagagag gcaaacatcc agcccgatca gatcggccag ttgtaagggc
     4621 cccatgggat gcgccatgcc cagtttcatg atggtatcaa tttcggttac tcctgccacc
     4681 ccttcatgca aacaataaat ggcctcattg atcatgggca tcaggatccg gttagagata
     4741 aatcctggaa aatcattgac cacgcagggt actttaccca ggtctttgct tagcgcaaca
     4801 atattgtccg tcacttcttt ggaagtgccc gggtgattga tgatct
//
LOCUS       DAOPMW010000252         4845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20379_length_4845_cov_7.581628, whole genome shotgun sequence.
ACCESSION   DAOPMW010000252 DAOPMW010000000
VERSION     DAOPMW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20379_length_4845_cov_7.581628"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1567
                     /locus_tag="PLU11_11250"
     CDS             110..1567
                     /locus_tag="PLU11_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPN59745.1"
                     /translation="MSKIQVKLVSLLAGVLVALFLYYVNPFHVAEPAARVLAVAGLMI
                     TWWVSEALPMPVVALLPLVLFPLLKINTMKSTAASYGNEVIFLFMGGFMLGLAIEKWN
                     LHRRIALNIVKATGTSGNRIILGFILATGFLSMWLSNTATTMMMFPIALSVIKVMGDK
                     QEGGNYAHFNLSMMLAIAWSSNFGGIATIIGTPPNVQFVGYFERKYNVNIDFVDWMIL
                     CTPLALILMFALYILTTKVLFPNRIRSSEAARKHIHGEIALLGKLSGPEKRVLLVFSV
                     TALLWIFKDLINLSQSMFKLDDNMIAIMGGLALFIFPAGKTELNGDHNKADGLLEWSD
                     TKDLAWGILLLFGGGIALADAMEKAGLITQLGAWLAQYAGGGIFLVFMVTFLSIFISE
                     VMSNVAQVIVFSPVVCSLADSMGMNPLLLGIPMTLAASCASMLPMGTPPNAIVFASGH
                     IRLKQMIKAGLVMNLVALVLITLFSWYLLPLLMKN"
     gene            complement(1738..2751)
                     /locus_tag="PLU11_11255"
     CDS             complement(1738..2751)
                     /locus_tag="PLU11_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPN59746.1"
                     /translation="MKKIFSTFLFCVSMATVSKAQDPNFSQFFASPLTLNPALTGKFD
                     GDFRVAGNYRNQWPTINNAFVTKTVSLDFGVMKNRLSELDQMGVGILGVTDRAGDGVL
                     TTNYAGLSLAYHKGLDEDGFHQLGAGFQGIYINKRLDVTKVQFADQLTPLGFTGVTSE
                     IFNNNQLNLSYFDLNAGVLYNGSTNGYNNFYIGASMYHINRPKESFQGGNYILNTRTT
                     IQAGGKIPMGSYNYLHIAANHSMQAKAYNTVVGGAFSYNVNNNEDDPVNVYIGAWYRL
                     KDAAIPYFGLEFKGLQVGFSYDANTSSLKPASNTRGGMEISMIYIKKHSDPNAKKLNC
                     PRF"
     gene            complement(3016..3666)
                     /gene="upp"
                     /locus_tag="PLU11_11260"
     CDS             complement(3016..3666)
                     /gene="upp"
                     /locus_tag="PLU11_11260"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPN59747.1"
                     /translation="MIVNLSQQHSLISNWVSELRDTQIQTDRMRFRRNLERIGEVAAY
                     EISKVLPAVEIEVSTPLGTAVHRVLKEYPVLATILRAGLPLHQGLLNYFDKSDNAFIS
                     AYRKHQPDGSFEISLEYMSSPELDDRVIIISDPMLATGSSLVKTIRFLKAEGQPREIH
                     IVAAIACTVGIEYVKREEPSVKIWCGDIDDELTAKGYIVPGLGDAGDLAYGPKMQM"
     gene            3822..>4845
                     /locus_tag="PLU11_11265"
     CDS             3822..>4845
                     /locus_tag="PLU11_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPN59748.1"
                     /translation="MVYRAIGLMSGSSLDGLDIVFAEFQEQGGQWSYELIEAGCLPYS
                     ADWKTRLSGATGLSARDYLLLHSEYGHYTGQQINAFIDQFNLHYKVAVIGSHGHTSFH
                     LPGQKMTAQLGDGSAIAAVTQLPVVSDLRALDVAFGGQGAPIVPIGEKLLFSGYDYFL
                     NIGGIANISGHANAYIAFDICPANRVLNLLAAEAGKEMDEGGAMAASGNIHHGLLGQL
                     NALDYYRQPWPKSLANDFGTDTVYPLIKGTGLTLADALCTYTEHIAIQTAEAVMAIQQ
                     QGKKENSRLLVTGGGAFNDHLLQRLDAHLSQLGVTTILPDPGLINYKEALIMAFIGVL
                     RWRQEYN"
BASE COUNT         1213 a         1102 c         1176 g         1354 t
ORIGIN      
        1 cgtaccggtt ttatataaag ggtaatccct atgtaagtgg ccgcaataaa acatagttca
       61 gcagccgatc cttttttcaa ataacttatc ttaccccaaa ttttcaggtt tgtcgaagat
      121 tcaagtcaaa ttagtttcct tactggccgg tgttctggtg gccctgtttt tatattacgt
      181 gaatccattt catgtagcag agcctgctgc ccgcgtgctg gcggtggccg gattgatgat
      241 cacctggtgg gtgtcggaag cattgccgat gccggtggtg gccttgttgc cattggtctt
      301 gtttccgctc ttaaagatca atacgatgaa atccaccgca gcttcttatg ggaatgaagt
      361 gatctttttg tttatgggcg gttttatgct gggactggcc attgaaaaat ggaacctcca
      421 ccgccggatt gccttgaata ttgtaaaagc aacaggcacc agtggaaaca ggatcattct
      481 gggtttcatt ctggctaccg gttttctcag tatgtggctg agcaatacgg ccaccaccat
      541 gatgatgttt ccgattgcac tctctgtcat aaaagtaatg ggtgataaac aggaaggagg
      601 taattacgcg catttcaacc tgagtatgat gctggctatt gcctggtcct ccaatttcgg
      661 aggcatcgcc accattatcg gcaccccgcc gaatgtgcag tttgtgggtt attttgaacg
      721 caagtataat gtcaatatag attttgtgga ctggatgatc ctgtgcacac ccctagcgct
      781 gatcctgatg tttgccttgt atatactgac tacaaaagtc ctttttccca accgtatccg
      841 ttccagtgaa gcagcccgta aacatataca tggtgaaatt gccctgctgg gcaaactcag
      901 tggtcccgag aaaagagtat tactggtgtt cagcgtgaca gcactcttgt ggatattcaa
      961 ggacctgatc aacctgagcc aatccatgtt taaactggat gataatatga ttgccattat
     1021 gggtggactt gccttattta ttttcccagc gggtaaaacg gaactgaacg gcgatcataa
     1081 caaagcggac ggattgctgg aatggagtga taccaaagat ctggcctggg gtattttatt
     1141 attatttggg ggcggaattg ccctggccga tgccatggag aaagcgggac tcatcaccca
     1201 gttaggtgcc tggctggctc agtatgccgg gggaggcatt ttcctggtat tcatggtcac
     1261 cttcctgtcc atttttatca gtgaagtaat gagtaatgta gcacaggtga ttgtattttc
     1321 cccggtcgtc tgttcattag ccgattccat gggaatgaat ccattattac tgggtatccc
     1381 catgaccctc gccgccagtt gtgccagtat gctgcccatg ggcactccgc ccaatgccat
     1441 tgtatttgcc agcggtcata tacggctgaa acaaatgatc aaggccgggc tggtgatgaa
     1501 tttagtagcg ctggtgctga ttacgttgtt cagctggtat cttttgccac ttcttatgaa
     1561 gaactaaaag ctttatagct atgaggaacg agcaacatgc cgtaaaagcg attcggtttg
     1621 ttatttaaga atcaacttac taaggagagg cagtttcctg acacccgaca cccgacatct
     1681 gacacctgtt ccccggctgt tcctaacacc taacagctaa caccgagcac cccttcttta
     1741 gaacctgggg caattcagct tcttcgcatt cggatccgaa tgtttcttaa tatagatcat
     1801 ggagatttcc ataccgccac gggtattgga agccggtttc aggctggagg tattggcgtc
     1861 gtaagagaag cctacttgta gtcctttgaa ctcaagaccg aagtaaggaa tggccgcatc
     1921 tttcaggcgg taccaggcac cgatatatac atttaccgga tcgtcttcgt tgttgttgac
     1981 attgtaggag aatgcaccac ccacaactgt attatacgct ttggcctgca tactgtgatt
     2041 ggcagcaata tgcaggtagt tataagagcc catcgggatt ttaccaccag cctgtatcgt
     2101 ggtacgggtg tttaagatat agttgccgcc ctgaaaactt tctttcggac ggttgatatg
     2161 atacatggag gcaccgatat agaagttatt gtaaccattg gtgcttccgt tatagagtac
     2221 accggcattg aggtcaaaat aactcaggtt gagctggtta ttattgaaga tttcactggt
     2281 taccccggta aagccaagtg gggtcagctg atcagcaaat tgtactttgg ttacatccag
     2341 tcttttgttg atatagattc cctgaaaacc tgcacccaat tggtgaaatc cgtcttcatc
     2401 cagtccttta tgataagcca gggaaagacc cgcataattg gtggtcagta cgccatcacc
     2461 ggcacggtca gtcacaccca aaatacccac acccatctgg tccagttcgg aaagacgatt
     2521 cttcatcacc ccgaaatcca gggaaacagt tttagtcaca aaagcattgt tgatcgtagg
     2581 ccattggtta cggtaattgc cggctacccg gaagtctcca tcaaacttac cggtcagcgc
     2641 cgggttcaat gtaagcgggg atgcgaaaaa ctgggaaaag ttgggatcct gcgccttgga
     2701 cacagtggcc atggacacac aaaacaggaa ggtggaaaag atttttttca tctctgcaat
     2761 taagtttttt cgttcaatct ggcaggaaaa gggatagcaa aaccgtaaaa aacactaaac
     2821 gtatattttc tccttttgat tgccccgtcc ttcaataatt ccgccggtac gaaccggtaa
     2881 aatacaactt tttaaagacc tgagaaatag aattaatgct gcttggcctg gttttgatat
     2941 gaatcattat atgtaaccgg atattttgtc taacaatctg gttataagat ttatacaaac
     3001 aaaaatctat attttctaca tctgcatttt cggtccgtag gccagatcac cggcatcgcc
     3061 gagtccgggt acgatatagc ccttggccgt aagttcatca tcaatatccc cgcaccagat
     3121 cttcaccgag ggctcttctc ttttaacata ttctatgcca acggtgcagg ctatggccgc
     3181 tacaatatga atttcacggg gttgtccctc tgcttttaaa aaacggatgg tttttacgag
     3241 ggaagaaccc gtggccagca tcggatcaga gatgataatc acccggtcat ccagttccgg
     3301 acttgacata tattccaggc tgatctcaaa actgccatcg ggctggtgtt tgcggtaggc
     3361 cgaaataaaa gcattgtccg atttgtcaaa atagttcagc agtccctggt gcagcggcaa
     3421 accggcccgc agaatggtag ccagtaccgg gtattctttg agcacccggt gtaccgcggt
     3481 ccccagcggg gtggatactt ctatttccac agcgggcagc actttgctga tttcataggc
     3541 cgctacttcc ccgattcgtt ccaggttgcg acgaaaccgc atccggtcgg tttgtatctg
     3601 tgtatccctt aattcactaa cccaattact gattaaagaa tgctgctggc tgagattgac
     3661 gatcatgtta agtcccggtt tggtaaccct ggcacaggaa tttgtaactc ccggtcctgg
     3721 ttttttgatt tttttctccc tgcctggcgg atgaagtata aagtcatacc gtaaagcatc
     3781 aggatcattt actttcgcaa aactaatcta aaaaagcttt catggtttac cgggctattg
     3841 gtttaatgag tggcagttcg ctggacggac tggatattgt ttttgctgaa tttcaggagc
     3901 aggggggaca gtggagttat gaactgattg aggcaggatg tcttccttat tcagcggatt
     3961 ggaaaacccg tctcagcggg gctaccggac tttctgcccg ggattatctc ctgctgcata
     4021 gtgaatatgg ccattatacc gggcagcaga tcaatgcctt tattgatcag tttaatctgc
     4081 attataaagt ggctgttatc ggttcacatg gccataccag ttttcacctg cctggacaaa
     4141 aaatgaccgc tcaactggga gatggttcag ccattgccgc ggtgacacaa ttgccggtgg
     4201 tctctgacct gcgggccctg gatgtggcat ttggtggcca gggagcaccg attgttccga
     4261 ttggtgaaaa attattattc agcgggtatg attatttcct gaatatcggc ggcattgcaa
     4321 atatttcagg tcatgccaat gcctatatcg catttgatat ttgtccggcc aaccgggtac
     4381 tcaacctgct ggctgcagaa gccggtaagg aaatggatga gggtggggca atggcggcct
     4441 cgggcaatat acatcacgga ttgctggggc aactgaatgc gctggattat taccggcagc
     4501 cctggcccaa atcactcgct aatgatttcg ggaccgatac ggtttaccca ttgatcaaag
     4561 ggaccggact tacactggcc gatgcgcttt gtacttatac agaacatatc gcgatccaga
     4621 ccgctgaagc cgtaatggcc attcagcagc aaggaaaaaa agaaaattca cgcctgctgg
     4681 tcacgggagg cggtgcgttt aatgatcatt tattgcagcg gctggatgcc catttatctc
     4741 aacttggggt gacaactata ttgccggatc ccggtctgat caattacaag gaagccctga
     4801 tcatggcttt tatcggggta ttacgttgga ggcaggagta caatg
//
LOCUS       DAOPMW010000253         4831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20462_length_4831_cov_8.331030, whole genome shotgun sequence.
ACCESSION   DAOPMW010000253 DAOPMW010000000
VERSION     DAOPMW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20462_length_4831_cov_8.331030"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="PLU11_11270"
     CDS             <1..1688
                     /locus_tag="PLU11_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc carboxypeptidase"
                     /protein_id="HPN59749.1"
                     /translation="HGWLYFTRERFDLLYPSYGDTYPTYNGSIGMTYEQGGIGAGLGV
                     IVEEGDTLTLVDRAQHHFTTSLSTIEIASLNAGRLVKEYRKFFNDATATGFGEHKSYV
                     IKYEARNQERFEQLIRLLDKNGIQYAAGTGAASKGFSYFSGKEESFSAGANDLVISAL
                     QPRSAMVKVLFEPKTKLSDSATYDITAWALPYVYGLNAYATKDKLAAAGAINLRTSVT
                     NPETTYGYVIPWNGIKTVKTVGHLLQKGILLRYAEQPFEVNGNKFERGAIIVLKTSNQ
                     KFGGDFWKMVRETTAADGVQIYAVSSGFVDKGFDFGSGMVHPMRMPKVVLVTGEGVSG
                     NSAGEIWHFFDQQLDFPVTLINANDLARANWNEIDVLIMPDGNYRFLSNKDELEAFKS
                     WVSKGGRVVALEGAVAQLAKADIGIKQKKTEDADKKDKSDSYEALQDYEDRERSALSG
                     YTPGSIWKVTLDPSHPLAFGYPDYYYTLKQDDIIYEFMKEGGWNVGVIKKENSVAGFV
                     GTALQPKLKDGMLFGSVQVGNGTVNLLVDNVMFRSFWENGKLMFCNAVFLAGE"
     gene            complement(1831..2229)
                     /locus_tag="PLU11_11275"
     CDS             complement(1831..2229)
                     /locus_tag="PLU11_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPN59750.1"
                     /translation="MIEPYSINELTAVVVDLCVKIHTKVGPGCYERVYEEILYYELTK
                     MGFNVKRQLLLPIQYEELFIPHAYKLDLLVEDRLVLELKSQHPLPLVFFNQIRTHLSF
                     LNLKNGMLLNFKVPRMKDGIFRIFNNSGLE"
     gene            2745..4649
                     /locus_tag="PLU11_11280"
     CDS             2745..4649
                     /locus_tag="PLU11_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPN59751.1"
                     /translation="MDKHEIIKWSLIIGIPLFLLLFYKFILRVCFGLVIVPEDRIGLV
                     TKQFVLFGQQELPEGRIIATKGEAGYQAQTLPPGVYFWKWIWQYSITFQPFIIIPTGK
                     IGLVLAKDGAELETGRILGRKVECDSFQDAEAFLHNGGRKGRQTSIIAPGSYRINTYL
                     FEVELTDMTMIPDNAVGIVTTAEGKPLEEGQIAGKIIEGHNKFQDADMFLQKEGLKGL
                     QEQVILAGSYFLNPWFARIEMVKMTEIPIGHVGVVISYVGTEGVDLSGVEFKHGNIVS
                     KGTKGVWAEPLGPGKYPINPYIMKVEMVPTTNLVLNWASARSESHQLDKNLSTITVRS
                     KDGFPFNLDVSQIIHIPTTEAPKVIARFGNMNNLVSQVLEPTIGNYFRNSAQDSDVIA
                     FLGTRKERQISAKEHIGKVLDQYNVYGVDTLIGDIVPPESLMKTLTDRKLAEEQKITY
                     ETEKRAQETRQTLEKETAIAEIQKEIVKADQGVLIAERIADASVKKATGDANSVRLQA
                     NAEADRLKLLASGEAEKTRLLAKADAEKIEWLAKAEAEKISLTGNAEAEKILAIGKSN
                     AESYKLSVEAMGGDNFTQLKVIESIAAEKVKIMPDVLIGGGDGANGGISGLLGLQLLE
                     KLGKKIGEKE"
BASE COUNT         1348 a         1005 c         1246 g         1232 t
ORIGIN      
        1 agcatggctg gctttatttt acccgggagc gttttgacct tttatatcct tcttacggcg
       61 atacctatcc cacctataat ggtagtatag gtatgactta cgagcaaggc ggtatcggtg
      121 ccggacttgg cgtgatcgtg gaagaagggg ataccctgac cctggtggac agggcccagc
      181 atcatttcac cacctcgctg agtaccattg aaattgcttc cctgaatgcg gggaggctgg
      241 tgaaagaata ccgtaaattt ttcaatgatg ctaccgcaac tggttttggt gaacataaaa
      301 gttatgtgat caaatatgaa gcccgaaatc aggaacgttt tgaacaactg atccgcttgc
      361 tggataaaaa tggtattcag tatgcggccg gaaccggagc tgcctcgaaa gggttcagtt
      421 atttcagcgg taaagaagag tcgtttagcg ccggagccaa tgacctggtg atctctgccc
      481 tgcagccacg ttcggccatg gtaaaagtgt tatttgaacc taaaacaaaa ttatctgact
      541 ccgctacgta tgatattacg gcctgggcat tgccatatgt gtatggactg aatgcttatg
      601 ccacaaaaga taaattagct gcagcaggtg cgattaatct gcgtacttcc gtaaccaacc
      661 cggaaaccac atatggatat gttataccct ggaatggaat taaaaccgtg aagacggtcg
      721 gtcatctttt acagaaaggc atcctgcttc gttatgcgga gcaaccattt gaagtcaatg
      781 gaaataaatt tgaacgcggt gccatcattg tcttaaagac ctccaaccaa aaatttggcg
      841 gagatttctg gaagatggtc cgcgagacaa ccgccgcaga tggcgtacag atttatgcgg
      901 tcagcagtgg atttgtagat aaaggctttg atttcggtag tggtatggta catccgatgc
      961 ggatgccgaa agtggtgctt gtaaccggag aaggggtgtc cggtaattct gcgggggaga
     1021 tctggcattt ctttgaccaa cagctggact ttccggtgac cctgatcaat gccaatgatc
     1081 tggcccgtgc caactggaat gagatcgatg tgttgatcat gccggatggc aattatcgtt
     1141 tcctcagcaa caaagatgaa ctggaagctt ttaagtcatg ggtcagcaag ggcggacgtg
     1201 tagtggccct ggaaggagcc gttgcccagt tagccaaagc agatatcggc attaagcaaa
     1261 agaaaacaga agatgcggat aaaaaagata aaagtgattc ttatgaggcg ctgcaagatt
     1321 atgaggatcg tgaacgcagt gcactttccg gttatactcc cggctcgatc tggaaagtaa
     1381 cgctggatcc gtctcacccg cttgcattcg gttatcctga ctattattat actcttaagc
     1441 aggatgatat tatatacgaa ttcatgaaag agggtggttg gaatgtcggg gtgatcaaaa
     1501 aggaaaattc tgtagctggt tttgtaggca ccgccctgca acctaagctg aaagacggga
     1561 tgctgtttgg ttcagtacaa gtgggaaatg gcacggtgaa tctgctggtg gataatgtga
     1621 tgttcaggag tttttgggag aatgggaagc tgatgttttg taatgcggtg tttttggcgg
     1681 gggaataggg gggagaatcg cctgcggcgg gcgctaaaga ggtcaaaaga ggaaaaaaga
     1741 gaatataaaa gagggcgtgc tattggtaat gcccattaag caacaataag ctaaacgtat
     1801 cagcttattt gaatctcaga tatccattct ctactctaat cctgaattgt tgaatattct
     1861 gaaaatgcca tctttcattc gtggtacttt gaagttcagc agcataccat tctttaaatt
     1921 cagaaaagaa agatgggtcc tgatctgatt aaagaaaacc aagggcagag ggtgttgtga
     1981 tttcagctca agtaccagtc tgtcttcgac cagcaggtca agtttatatg catgtggaat
     2041 aaataactcc tcatactgta ttggaagcag aagctgtctt tttacattaa accccatctt
     2101 tgtcagttcg taatatagta tctcttcata tacccgctca taacatcccg gaccgacttt
     2161 agtgtggatt ttaacacaca gatcaactac gactgcagtt agctcattta tagagtatgg
     2221 ctctatcata aaaatgtttt tttgatgtat ttgttttttg cctgttaaaa gtatagtggt
     2281 ttttatttta tatcaggcac cgtcgccgac tacagacaaa ttaatttaaa gcgtttaggg
     2341 gttgtcgttt agaaagtcat tgccaataac aggctctctt tttctccttt tctcttttga
     2401 cctctttagc gcccgccgca ggcgcgtctc aaaaggtctc ttccggtttt tttgtggatt
     2461 attcaacttt gttgtaattt tactccttca aacaagcaaa atgaaccccg aatcagacca
     2521 tacggtcttc tcttctatcg agtccgaaat acggcacacg atttacaata cttctccacc
     2581 ccttcagtaa aaagaaaatg gttaaacagt tgatcttgta aagacggagt cgtctttcag
     2641 ggcaacgtgt atcatgtcga aaacttgtgt gcattttctg ctacaggtca taggctgctg
     2701 tgtgcatatc agataatttc aataaccctt aatcaataat caacatggac aaacatgaaa
     2761 tcatcaagtg gagcctcatt atcggcatcc ccttattctt attgcttttt tacaaattta
     2821 ttctccgggt atgcttcgga ctggtcattg taccagaaga ccgtatcggg ttagtaacaa
     2881 aacaattcgt gcttttcgga cagcaggaat taccggaagg acgtattatc gccaccaaag
     2941 gcgaagcggg ttaccaggcg cagaccttac ctccgggtgt gtatttctgg aaatggatct
     3001 ggcagtattc catcactttt cagccattta tcattatccc gaccggtaag atcggactgg
     3061 tactggcaaa ggacggtgcg gaattggaga caggtcggat cctcggacgt aaagtggaat
     3121 gtgattcttt ccaggatgca gaggcgtttc tgcacaacgg tggacgaaaa ggccggcaga
     3181 cctctatcat tgcccctgga tcctatcgta tcaataccta cctgtttgag gtggaactga
     3241 ccgatatgac catgatcccc gacaatgcgg tgggtattgt aaccacggct gaaggaaagc
     3301 ccctggaaga aggacagatt gccggtaaaa tcattgaagg gcataacaag tttcaggatg
     3361 cggatatgtt ccttcaaaaa gagggcttaa agggtttgca ggagcaggta atcctggccg
     3421 gttcttattt cttaaacccc tggtttgccc ggatagaaat ggtgaagatg accgagattc
     3481 ctattggaca tgtcggagta gtgatttcct atgtaggaac agaaggcgtg gacctgagtg
     3541 gtgtggaatt taaacatgga aatattgttt cgaaagggac gaaaggcgta tgggccgaac
     3601 cgttaggtcc gggaaaatac ccgatcaacc cttatatcat gaaggtggaa atggtgccca
     3661 ctaccaacct ggtgctgaac tgggccagtg caagaagtga gagtcatcag ctggataaaa
     3721 acctgtccac gatcacggtc cgcagtaagg acgggttccc gttcaatctc gacgtatcgc
     3781 agatcatcca tattccaacc accgaagcgc ctaaagtaat tgcgcggttt ggtaatatga
     3841 ataacctggt gagccaggtg ctggaaccaa ccattggtaa ctatttccgt aacagtgccc
     3901 aggacagcga tgtgatcgcc ttccttggaa cccgtaagga acggcagatt tctgctaaag
     3961 agcatatcgg caaagtgctg gatcaatata atgtgtacgg ggtggatacc ttgattggtg
     4021 atatcgtacc gccggagagc ctgatgaaaa ccctgaccga tcgtaaactg gccgaagagc
     4081 agaagatcac gtatgaaacc gagaagaggg cgcaggaaac ccggcagacc ctggagaaag
     4141 aaaccgccat cgctgaaatc cagaaggaga tcgtaaaggc ggatcagggt gtactcattg
     4201 cggaacgtat cgctgatgct tctgtgaaga aagcgaccgg tgatgccaac agtgttcgtc
     4261 tgcaggcgaa tgcggaagct gaccggttga aattactggc cagtggtgaa gcggaaaaaa
     4321 caaggctgct tgccaaagcg gatgcggaga aaattgaatg gctcgcaaaa gcagaagcgg
     4381 agaaaatctc acttactggt aatgcggaag cagaaaagat cctggcgatt ggtaagtcaa
     4441 atgcggaatc ctataaactg tccgtagaag ccatgggtgg cgataatttc actcagctga
     4501 aagtgatcga atccattgct gcagaaaaag tgaagatcat gccggatgtg ctgatcggtg
     4561 gtggtgacgg ggccaatgga ggtatcagtg ggttactggg actgcagttg ctggagaaac
     4621 tggggaagaa gattggagag aaggaatagt tagtagttag tagataggag ttagtagtca
     4681 acagccaaca gccgcagcgc ccgccacgcc accagccgct tcctcggcgt cgcctgggac
     4741 cgccgccggc aaaagtgggt cgcctatatc aagaaacacg gccgccagaa gaacctcggc
     4801 ctcttcgacg tcgaagaaga cgccgcccga g
//
LOCUS       DAOPMW010000254         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20538_length_4813_cov_6.545607, whole genome shotgun sequence.
ACCESSION   DAOPMW010000254 DAOPMW010000000
VERSION     DAOPMW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20538_length_4813_cov_6.545607"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1295)
                     /locus_tag="PLU11_11285"
     CDS             complement(87..1295)
                     /locus_tag="PLU11_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPN59752.1"
                     /translation="MKLIPIVFLAFFLHFCGSPRQVQSEKETRILTGADQTEKYLPLL
                     NGKRVAIMANPTTVIGNTHLVDSLQKRGVNIVKVFGPEHGFRGNASAGVHVADETDPI
                     TGIPVISLYGAKNKPSKQDLADVDILLYDLQDVGCRFYTNINALARLMDACYENGKEM
                     LILDRPNPNGYFVDGPVLDMKFKSGIGMFPIPIAHGLTVGEFAQMVNGEGWLSNKVKC
                     PVTIIPVANYTHDMPYTLPVKPSPNLNTQQSILLYPSTCLFEGTYLNHGRGTYFPFTI
                     IGSPALKGKYEFSFTPTGIKGMSETPLFMNQLCYGLDLRNYDVAELRKTKQINLQWMI
                     ELYKASPNKEQFFDNKLSKEMGVIEKLTGHALFRQQIMEGKSEKEIRASWEPGLSAYK
                     TTRKKYLLYP"
     gene            complement(1442..1942)
                     /locus_tag="PLU11_11290"
     CDS             complement(1442..1942)
                     /locus_tag="PLU11_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59753.1"
                     /translation="MTFTASLLKEMENEARTTRKILERVPDGNYDWKPHEKSMSIRQL
                     ATHVAEITGWIPLILNTDEMDFLKMEYKPAAINNQQELMTHFEKSLEEGKSALAAVTD
                     EKLINETWTMRSGDTIHSAESRLDNVRHCFCQIVHHRAQLGVYLRLLNIPLPGSYGPS
                     ADDNSF"
     gene            complement(2008..2697)
                     /locus_tag="PLU11_11295"
     CDS             complement(2008..2697)
                     /locus_tag="PLU11_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPN59754.1"
                     /translation="MNRMDRLLGMLTLLQSRKYVTAEKIAAKFSISVRTVYRDVKALG
                     ELGIPVSFEAPKGYFIVQGYFLPPVSFSTEEANALLLIEVLVNGFTDQSVRKQYASAL
                     DKIRSVLRGHQKDQLELLNDKIRFQKPACYDLHFEYLPTLQNAIAARSVIELEYLNQK
                     EENSKRKVEPIGLIFYAFNWHLIGWCHSRRDYRDFRVSRIKKITDTGQPFRKKEHMEL
                     NEYMKQLPVNY"
     gene            complement(2758..4062)
                     /locus_tag="PLU11_11300"
     CDS             complement(2758..4062)
                     /locus_tag="PLU11_11300"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPN59755.1"
                     /translation="MKIQTTRLPHRVQLKLSSSAWLAISYLALSIVWIMLGDSMASQI
                     SGSDQVLYEKIQSLKGIFFILVSAVFLYLFSRKLYLGIELSHLQTESVQKKYQALNEA
                     AREGLFEYDYKENKASLNTKMKFFFPAEGNELPDFWQNCQRRIHADDLDRLSREYEEI
                     IRTYKQIWKSEFRLLGVDGKFYRVISNVYLIRHSSSNEIIRMIGAIQDITDLRSLQTD
                     YYEQKLKHRRTLAASIIQAQETERTRWAEELHDNVCQILSVANMYAIDIADHPENLPN
                     LGPQLKKLVAESINEIRQLSATIRTPAFDQETLVESIEKLSANINRLKPVSIELDNQS
                     LNEGELENDKKLMIYRVVQEQINNIIKYAEARSVEIKLDNTSSNLVLISVRDDGKGFD
                     PEKVRTGIGLRNIQSRLQVYNGSLKINTSPGNGCLLEASFPN"
     gene            4340..>4813
                     /locus_tag="PLU11_11305"
     CDS             4340..>4813
                     /locus_tag="PLU11_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59756.1"
                     /translation="MLKRLSSAIIAFFTLTVSLSAQVERVQEQGPGISARLQYEIDIT
                     KDPSLGYVPKGRLVEAYQQRQERLQNLANRATAFTWTERGPNSDVTGPSNGNTRPGNG
                     ITSGRVRAIWEDLGDVTRKTVWVGGIDGGLWKTTDITATPATWTPINDFFGNLAVG"
BASE COUNT         1215 a         1065 c         1149 g         1384 t
ORIGIN      
        1 ggagaataaa aaagccgccc cgggtggcag gcggttttat tattcttttt aaaaataatc
       61 aggagtcgta ctgcaagcag tttgctttac ggatacaaca ggtatttttt ccgtgttgtt
      121 ttgtatgcac tcagaccagg ttcccaactg gcacggattt ccttttcgga ttttccttcc
      181 atgatctgct gacggaataa cgcgtggcca gtaagttttt caatgacccc catttcctta
      241 cttagtttgt tgtcgaaaaa ctgttcctta ttcggcgagg ctttatatag ctcaatcatc
      301 cattggaggt tgatttgttt tgtttttctt aattcggcca catcataatt gcgcaggtcc
      361 aggccataac aaagctggtt catgaataag ggtgtctccg acataccttt aatgccggtg
      421 ggggtaaaac tgaattcgta ttttcctttc aatgccggac tgccgatgat agtgaagggg
      481 aagtaggtgc cccggccatg attcaggtat gtgccttcaa aaagacaagt ggatggatag
      541 agtaaaatgg attgctgggt attcagattc ggggaaggtt ttaccggcag ggtatagggc
      601 atgtcatgtg tataattggc caccgggatg atggtgaccg gacatttgac tttattggac
      661 agccagcctt ccccattgac catttgtgca aattcaccaa cggtcagacc gtgggcgata
      721 gggatcggga acatgccaat gcctgatttg aatttcatat ccagcacggg gccatctacg
      781 aagtatccgt tcggattagg ccggtcaagg atcaacattt cttttccgtt ttcataacag
      841 gcatccatca gccgggcgag cgcattgata ttggtataaa agcggcatcc cacatcctgc
      901 agatcatata aaagaatgtc tacatcagcg aggtcctgtt tggacggttt attttttgca
      961 ccatacaaag agattaccgg tataccggta atcgggtctg tttcatcggc cacatgcacg
     1021 ccggcactgg catttccccg aaagccatgt tcgggcccaa aaactttaac aatattcacc
     1081 ccgcgtttct gcagactatc caccaggtgc gtgttaccga ttacggttgt agggttggcc
     1141 ataatggcca cccgctttcc gttgagcaac ggtaaatatt tttcagtctg gtcagcgcca
     1201 gtgagtatcc tggtttcttt ttctgattgc acttgccggg gacttccgca aaaatgaaga
     1261 aagaaggcaa ggaatacgat ggggatcagt ttcatgttag tgaattgaac cttaaaaata
     1321 aggaatattc aatcctgact gttgaggctg acaggttgca aaaaaagaaa tacgtattag
     1381 actcatctga aatatatgaa aatgcagggc ttacttccaa tcgatgtaag ccctgttttt
     1441 atcagaagct gttatcatcg gcgctggggc cataactacc cggcagtggt atatttaaca
     1501 gccgcaggta aaccccgagt tgtgcccggt gatgtacgat ctggcaaaag caatgccgca
     1561 cattatcaag cctggattcg gcactgtgaa tggtatcccc gctccgcatg gtccaggttt
     1621 cgttaatcag tttttcatct gtcactgcag ctaatgcact ctttccttcc tccagcgatt
     1681 tttcgaaatg ggtcatcagc tcctgctgat tattaatggc agcaggcttg tattccattt
     1741 tcaaaaaatc catttcatcc gtgttcagga tcaggggaat ccacccggta atttcagcta
     1801 catgggtggc cagttgccgg atactcatac ttttttcgtg gggcttccag tcgtaattcc
     1861 cgtccggaac ccgttccaga attttacggg tggtacgggc ttcattttcc atttctttca
     1921 ataaggaggc agtaaatgtc atgttgtttg attttagact acaaaacaaa catgccccac
     1981 tgacaaccct atggcagccg caaaaaatta ataattcacg ggtaattgtt tcatgtactc
     2041 attgagttcc atgtgttcct ttttacggaa tggctgaccg gtgtccgtga tttttttgat
     2101 ccgggaaacc cggaagtccc ggtaatcccg tcggctatgg caccagccga tgaggtgcca
     2161 gttaaacgcg taaaaaatca gtccgatggg ttcgactttc cgtttgctgt tttcctcttt
     2221 ctgattcagg tattcaagtt ctatgactga ccgggcggca atggcatttt gcagggtggg
     2281 gaggtattca aaatggagat cataacaggc cggtttctga aaccggatct tgtcattcag
     2341 caattccagt tgatctttct gatgccccct gagtaccgaa cggattttat ccagggcgct
     2401 ggcatattgt ttacgtacag actgatcggt gaatccgttg accaatactt caatcagcag
     2461 gagggcatta gcttcttcgg tgctgaagga cacgggtggc agaaaatagc cctgaacaat
     2521 aaaatagcct ttcggcgctt caaaacttac cggtataccc aattcgccca atgcttttac
     2581 atcgcggtag acggtacgga cactgataga gaatttggct gcaatttttt cagcggttac
     2641 atattttcgt gattgcaaca gtgtgagcat gcccagcagg cgatccatcc ggttcatagt
     2701 ataaatatag tggaattcaa caggcttttc cagcggggat aatacatagc gcctggttta
     2761 attcgggaaa ctggcttcaa gcaaacagcc attacccggt gaggtattta ttttaagact
     2821 gccattataa acctgcaaac ggctttggat attccggagg ccgatgccgg ttcttacttt
     2881 ctccgggtca aaccctttgc catcgtcacg gacggaaatc agcaccagat tactgctggt
     2941 attatccagt tttatttcta ccgaccgggc ttccgcatat ttgatgatat tgttgatttg
     3001 ttcctgtacc acccggtaga tcatcagctt cttatcattt tccagttcac cttcattcag
     3061 cgattgatta tccagctcaa tgctaaccgg tttcagccgg ttgatattgg cggatagttt
     3121 ttcaatggat tccaccaaag tttcctgatc aaatgcaggc gtgcggatcg ttgcggaaag
     3181 ctgccggatc tcattgatac tttcagctac cagttttttt aattgtggtc ccaggttcgg
     3241 cagattctcc ggatggtcgg cgatatcgat tgcatacata ttcgctacag agagaatctg
     3301 gcacacattg tcatgcaact cttcggccca gcgggttctt tctgtttcct gtgcctgtat
     3361 aatggaagca gccagtgtcc gcctgtgctt taatttttgt tcatagtaat cagtctgcag
     3421 gctgcgcaga tcggtgatat cctgtatggc gccgatcatc cggatgattt cattactgga
     3481 ggagtggcgg atcaggtaga cattactgat cacccggtag aatttcccgt ctacccccag
     3541 taatcggaat tcggatttcc agatctgttt gtacgtgcgg atgatttcct catattcccg
     3601 ggaaagacga tccaggtcat cggcatgaat acgacgctga cagttttgcc agaaatccgg
     3661 caactcattt ccttctgcgg ggaaaaagaa tttcattttt gtattcaggc tggctttgtt
     3721 ttccttgtaa tcatattcaa acaggccttc cctcgccgct tcattcagtg cctgatattt
     3781 tttttgtaca ctttctgttt gcagatgaga gagctcaatt cccaggtaga gtttccggct
     3841 gaacaggtaa agaaaaacgg cggataccaa aataaaaaag atgcctttga gggattgtat
     3901 tttttcatag agtacctggt cactgccgga tatctgggat gccattgagt cgccaagcat
     3961 gatccagacg atactcaggg ccagatagct gatggccagc caggcggagg aggataattt
     4021 caattgcaca cgatggggca atcgggtggt ctgaattttc atgcggtggt tttgttaaac
     4081 gaaagctaag ataaatctgc gtataaacga attctatgac ctatgtagtt atgcttatga
     4141 tttacctcat tgcgaatggt tgattttggg gaacagcctg agaatcagct gtgttttttt
     4201 agcttatttc atggacttta gataggtaat gaaggatagc catggaaaaa ggggtattaa
     4261 tatctggtag acgggcttgt cagggtaaaa aaaaaagtaa aatattagac tttatatttc
     4321 cctcaccaaa aaccaatcta tgcttaagcg actcagctcc gcgattattg cattttttac
     4381 attaaccgtt tccctttccg cacaggtgga aagagttcag gaacaaggcc cgggaatttc
     4441 agcccgtttg cagtacgaaa tcgatatcac caaggatcct tctttgggtt atgtgcctaa
     4501 gggacgatta gtggaagctt atcagcaacg ccaggaacgt ttgcaaaacc tggctaacag
     4561 ggccacggct tttacctgga cggaacgagg accgaacagc gatgtgaccg gtcccagtaa
     4621 cggcaatacc cgtcccggaa acgggataac ctcaggaagg gtccgggcaa tttgggagga
     4681 ccttggagat gtgacccgta aaacagtgtg ggtaggcggg atagatggcg gattatggaa
     4741 aacgacagat attacagcaa caccggcaac ctggacaccc atcaacgatt tctttggcaa
     4801 cctggctgtg ggt
//
LOCUS       DAOPMW010000255         4780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_20740_length_4780_cov_6.313228, whole genome shotgun sequence.
ACCESSION   DAOPMW010000255 DAOPMW010000000
VERSION     DAOPMW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20740_length_4780_cov_6.313228"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..747)
                     /locus_tag="PLU11_11310"
     CDS             complement(154..747)
                     /locus_tag="PLU11_11310"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HPN59757.1"
                     /translation="MPDVFDTLINSYISHNTGIDPFFLSEELANGLRLHIITLQHAGL
                     LTAAGIGNKENKGIHEEIRNDRIYWLDKSNHNSFEQEFLQLADEFIACLNQSCYTGIN
                     ACEFHYAIYDNNSFYKRHLDQFRNDGNRKFSLITYLNQDWTVADGGQLQIFQGDQTQQ
                     ILPHSRTAVFFKSDELEHEVMVTHRQRLSVTGWLKRV"
     gene            complement(750..1301)
                     /locus_tag="PLU11_11315"
     CDS             complement(750..1301)
                     /locus_tag="PLU11_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59758.1"
                     /translation="MTSNSIIHFIGFVTRLESEKFIPAWEQYAKKEMFRKKAPLLLQQ
                     DNGSRNKFNFLSLHIWPDTETHFSFTKSDKAGYFHEMPVQVVHIGGYLPLEQTNTSLH
                     KDTECRLLVMAGHNENDPAFYKTLPYSFRLNSYQAYYESCTYGYIFEYFAAEQEMEEI
                     MNVLKQRAGTERILYRDCFATHI"
     gene            complement(1319..1744)
                     /locus_tag="PLU11_11320"
     CDS             complement(1319..1744)
                     /locus_tag="PLU11_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN59759.1"
                     /translation="MATVLELSQADKNLVSFMKAIKASGMEDELKAVGPFTFLAPVNL
                     AFGKLSQGDLFETLLKQDGSRLRAILSYHIIKGKKMQRDFRTGQKVQTLGGQELQVTT
                     RDGDIYINNARILAKDRQGSNGVVHCIDSINLPPELKPV"
     gene            complement(1872..2141)
                     /locus_tag="PLU11_11325"
     CDS             complement(1872..2141)
                     /locus_tag="PLU11_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPN59760.1"
                     /translation="MNLYVSNLSFHTADNELRTLFEQYGAVSSAKVIMDRESGRSRGF
                     GFVEMSSDNEANEAIKNLHQKEIEGRAMSVTVAREKAPRTENKRW"
     gene            2444..2830
                     /locus_tag="PLU11_11330"
     CDS             2444..2830
                     /locus_tag="PLU11_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59761.1"
                     /translation="MKFVIFLLLWSGISLNSSAQGKSRVHVVTTWDNRQASLKLSFSI
                     NGTVYKFLPGKCLELELTSDSLHVVMTDTRWVKKETVDLHIPIDDDLYVHVFWGRKTE
                     DPKWMTRAIAEQVCKDCFDELKKKCR"
     gene            2960..3694
                     /locus_tag="PLU11_11335"
     CDS             2960..3694
                     /locus_tag="PLU11_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59762.1"
                     /translation="MTAISFLLLFSSCEKKPACAGSLTLTANTLTPTVGDNIVISAPD
                     RGTTNVVYQWNGPGTNLTNQSNTLEINDIKLSQSGVYSLNEGNSDCNTSLSDTIRINV
                     QLKQETPPCTNTNNTATPSSIPATTFTTVNKSFSSTWNTIALEASGSFGYPSYTVLFN
                     SYNGNTEPRDGVYTTADVAIFNPLQEPNEVSVSFIYSSNYFHCRPGNKVYVSHVNGKL
                     QVSFCNLEFSSPPFVTSVSAKITQLN"
     gene            complement(3691..4341)
                     /gene="hxpB"
                     /locus_tag="PLU11_11340"
     CDS             complement(3691..4341)
                     /gene="hxpB"
                     /locus_tag="PLU11_11340"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HPN59763.1"
                     /translation="MEYKAAIFDMDGLLIDSEPLWQEAGIETLSGFGISLTLEQYHTS
                     TGLRTEEWVAHWFRQYQVSPAHAPEAVQVIIRKAIEKIAANGIALPGATEILGFFNKQ
                     NIRTGLATSSPMSLVDVVVDKLHIRDQFQVFTSAEYLPYGKPHPMVFLECAKSLDVLP
                     EDCIVFEDSFNGMIAAKAARMKCVVVPAPEQADLPKWGAADMKLLSLNEFLTYNSF"
     gene            4469..>4780
                     /locus_tag="PLU11_11345"
     CDS             4469..>4780
                     /locus_tag="PLU11_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59764.1"
                     /translation="MKKFLLILMVIAVLHAVAQKGRFHTGLIVTTSGDTLKGTISWKK
                     NPGRLDSLLYKKNIQSDIVSYAWSSLTFFATSGGEEHIVRTVKRNLEYIDPYTFNINL
                     AD"
BASE COUNT         1286 a         1013 c         1059 g         1422 t
ORIGIN      
        1 ctggcgtgag ccgacgctgc tatgctttat gtctggcgta agccgacgct gctatgcttt
       61 atgtctggcg taagccgacg ctgctatgtt ttatgtctgg cgtgagccga cgctgctatg
      121 ctttatgtct ggcgtaagcc gacgctgcta tactcacact cttttcagcc agcccgttac
      181 actcagccgt tgccggtggg tgaccatgac ttcatgttcc agttcatcgc ttttgaaaaa
      241 cacggcggta cgggaatggg ggagtatctg ctgggtttga tcaccctgga aaatttgcaa
      301 ctgccctcca tcggctacgg tccagtcctg gttcagatag gtaatcaggg agaattttcg
      361 attgccgtca ttccggaact gatccaggtg gcgtttgtaa aaactgttat tgtcataaat
      421 ggcgtaatga aattcgcagg cattgatacc ggtataacag ctttggttca gacaggcgat
      481 aaattcatca gccagttgca ggaactcctg ttcaaatgaa ttgtgattgc ttttatcgag
      541 ccagtagatc cggtcattcc ggatttcttc atggattcct ttgttctctt tattaccaat
      601 gccggctgct gttaacagac cggcatgttg taatgtaata atatgtaatc gcaggccatt
      661 ggccagctct tccgataaaa aaaatggatc gattcctgta ttatggctga tatagctgtt
      721 gatcagggta tcgaatacat ctggcaaagt cagatatggg tggcgaagca atccctgtac
      781 aggatacgtt cagtgccggc cctttgttta agaacgttca tgatctcttc catctcctgc
      841 tctgcggcaa aatattcaaa gatgtatccg taggtacagc tttcatagta ggcctggtaa
      901 ctgttgagtc ggaaggagta gggcagtgtt ttataaaaag ccggatcgtt ttcattatgt
      961 ccggccatga caagtaaacg gcattctgta tccttgtgca gactggtatt ggtttgttcc
     1021 aatggcaggt aaccaccgat gtgaactact tgtaccggca tttcatggaa ataaccggct
     1081 ttgtctgatt tggtaaagct gaaatgcgtt tcggtatccg gccagatatg gagggataga
     1141 aaattgaatt tattccggct gccgttatcc tgctgaagca acaagggtgc ttttttccgg
     1201 aacatttctt tttttgcata ttgttcccag gccggaataa atttttccga ttccaggcgg
     1261 gttacaaatc cgataaagtg tattatacta tttgacgtca tactgatctt taaattattt
     1321 aaacgggttt cagttcaggg ggcaggttga tgctgtcaat acaatggacc acaccattgg
     1381 aaccctgacg gtctttagcc agtatacggg cattgttgat ataaatatcc ccgtcccggg
     1441 tggtgacctg gagttcctgt ccgccgagcg tttgtacttt ttgcccggtt ctgaaatcac
     1501 gctgcatttt ttttcctttg ataatgtgat aggagagtat ggcccgcagc cggctgccat
     1561 cctgctttaa taaggtttca aacaaatcac cctggctgag tttgccaaat gcgagattga
     1621 ccggggccag aaaggtaaaa ggccctacgg cttttaattc atcttccatt ccggatgctt
     1681 ttattgcttt cataaaagaa accaggtttt tatctgcctg tgataattcg agtactgttg
     1741 ccatagtaat agtttattgc agcaaaaaac cgcaagcagg tactgctcag gatcaggctg
     1801 gtgcatgaat cggatgtaat acggaaaagg gaagaaataa agagctgaat catcagctct
     1861 ttatgctgga attaccatct tttattttct gtgcggggag ctttttcccg ggcaacagtc
     1921 acggacatgg cgcgtccttc gatttctttc tggtgcaggt tcttgattgc ttcattggct
     1981 tcattatcag aagacatttc cacaaatcca aaaccacggc ttctgccgga ctctctgtcc
     2041 atgatcactt tagcagaact tacggcgcca tattgctcga ataaggtgcg gagttcattg
     2101 tctgcagtgt ggaagctaag attagaaaca tacaaattca taatacaatt ttttaaataa
     2161 acaatagaaa ataaggtgaa aggaactgct gcacgaagta gagaggagag gaattagtac
     2221 aatggatgtc tctgtaacaa ttaatgtcga tcagaactca atgtaggaag aacttatttc
     2281 actaaagtga agatacgctt ttatacggtc attgctgttc ctattttcaa gcgggatgag
     2341 ttttaacatg tttgcttaag aaaaggaagc taagaggctc attcttagcc ttgtgacaac
     2401 agttgttttt tttgctgttt aactgatcaa ttatatttga tggatgaagt ttgtcatttt
     2461 tctgttatta tggagcggca tttccctgaa ttcaagtgca cagggcaaga gccgggtaca
     2521 tgttgtaacc acatgggata atcgtcaggc aagtctgaaa ctcagttttt ctattaacgg
     2581 gactgtgtat aaattcctgc cgggcaaatg tcttgaattg gaattaacat cagatagcct
     2641 gcatgtggtt atgactgata cccgctgggt taaaaaagaa acagtggatc tgcatattcc
     2701 catcgatgat gatctatatg tacatgtatt ctggggcaga aaaacagaag atcccaaatg
     2761 gatgacccgg gcgatagccg aacaggtctg taaagactgt tttgatgagc taaagaaaaa
     2821 atgcaggtag caaactgatc tgaatcaatt accttttcag gagataaaac cgctattgat
     2881 agttttgttt tttcaccatt gtatttacgg tatccgttct atatattcgc attcaaatta
     2941 ttttatgaaa atttctgttt tgactgcgat atcatttttg ctgttgtttt cttcctgtga
     3001 aaagaaaccg gcctgtgcag gttcacttac acttacagcc aataccctga cacccacagt
     3061 gggtgacaat atagtcatct cagctccgga ccgtggtacc accaatgtcg tttatcaatg
     3121 gaatggacca ggaaccaatc tcaccaatca atccaataca cttgagataa atgatattaa
     3181 gctatcccag agtggggtct atagtttgaa tgaaggaaac agtgattgta atacatcatt
     3241 gagtgatacc atccgtatca atgtacagtt aaaacaggaa accccgccct gtactaatac
     3301 caacaatacg gccaccccgt ccagtatccc ggctactact ttcaccaccg tgaataaatc
     3361 tttcagcagt acctggaata cgattgcgct tgaagccagt ggcagtttcg gttatccttc
     3421 ctatacggta ttgttcaact cttataacgg aaatacagag ccacgggatg gtgtttatac
     3481 cacagccgat gtagccattt ttaatccgct gcaggaaccc aatgaagtgt ccgtcagttt
     3541 tatctattcc agcaattact ttcattgccg gccaggtaat aaagtttatg tgtcacatgt
     3601 aaatggcaaa cttcaggtca gtttttgcaa tcttgagttt tcgtctccgc cttttgttac
     3661 gtcagtctct gccaaaatca cacagttaaa ctaaaaggaa ttataagtaa gaaattcatt
     3721 cagactgagc aatttcatgt ccgccgcccc ccatttcgga agatccgcct gttcaggtgc
     3781 cggtaccacc acacatttca tcctggcggc ttttgctgcg atcattccat tgaacgaatc
     3841 ttcaaataca atacaatctt cgggtaacac gtcaagcgat ttggcacatt ccaggaagac
     3901 catcggatgt ggtttgccat aggggaggta ttcggcggat gtgaaaacct ggaactgatc
     3961 ccggatatgt agtttgtcga ctaccacatc taccaatgac ataggggaag atgtcgctaa
     4021 tccggttctt atattttgct tattgaaaaa gccaagaatc tccgtagcac caggcaaagc
     4081 aatgccatta gctgcaattt tttcaatcgc tttgcggatt atcacctgaa ctgcttccgg
     4141 tgcatgtgca ggagaaacct ggtactgcct gaaccaatgc gccacccatt cttccgtacg
     4201 aagtcctgtg ctggtatggt attgttcaag ggtcagactg attccaaatc cggagagcgt
     4261 ctcaatcccg gcttcctgcc agaggggctc actgtctatc agcaaaccat ccatatcaaa
     4321 aatcgccgct ttgtattcca tgctgcaaac attcaacaag caaaggagga ttccaaaaaa
     4381 ataagtgatt tcctgataag gaagtgctgc tttgcaaaaa actcaggttt ttttttattc
     4441 tgtgggaatg tatatttgta cacctgctat gaaaaaattc cttttaatat tgatggtaat
     4501 tgcagtttta catgctgttg cccagaaagg ccggtttcat accgggctga tcgttaccac
     4561 gtcaggggat accctgaaag gcaccatttc ctggaagaaa aatccggggc gattggatag
     4621 tttgctgtac aaaaaaaata ttcaaagcga tattgtttcc tatgcatggt cctcgctgac
     4681 tttttttgcc acatccggcg gagaagaaca tattgtccgt accgtaaagc gtaacctgga
     4741 gtacattgat ccctatacgt ttaatattaa tttagcggac
//
LOCUS       DAOPMW010000256         4683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21364_length_4683_cov_5.104797, whole genome shotgun sequence.
ACCESSION   DAOPMW010000256 DAOPMW010000000
VERSION     DAOPMW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21364_length_4683_cov_5.104797"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1909
                     /locus_tag="PLU11_11350"
     CDS             290..1909
                     /locus_tag="PLU11_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPN59765.1"
                     /translation="MKKKAYNQLIVLFLPILVIAQQNEPAWDLTANKSWPLPFQQVQI
                     KSTMDNHLQQAWYYESTGNPLQPLIISLHTWSGDYNQEDPLAREALLRNWNYIHPDFR
                     GANNKPEACGSPLVIADIEDAIRFAIQKGKVDTHNIHIIGVSGGGYTALLAYMKIRYP
                     VKSFSAWVPISDLRSWYWESRGRKAKYADDVEKVAMKNGAMDWPDLDSRSPIHLLYPA
                     SLRKNAELHIYAGVHDGYTGSVPISQSMQFYNKIAAAIYPTRKHLLITETLQQSLLAK
                     RINPAADSTLKLNDRQIHLFRKTDRISITLFEGGHEMLVPSALTLIPADNNKNNGNLN
                     ILAIGDSNGAAADGWPNQLQKLLPYSTIINTCVSGNTIGFDNLGQEKLNTLKNITRYL
                     EDTYRQLGPEGQLDYILIGLGTNDTKAIFKEQQKEVPANLSKLLDSINAFIHTRKLKA
                     PAICLLLPPPVDEQKADSVKYGGSADRLLLNNPLLKKVADTHKAATIDLYQLLSSTTS
                     ELTTDGIHLTEMAQYKVAGFIVHRLNTSHKK"
     gene            1906..3675
                     /locus_tag="PLU11_11355"
     CDS             1906..3675
                     /locus_tag="PLU11_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPN59766.1"
                     /translation="MRTFISTWTLLLLITFPCSHTWSQQNKNSRYIVADTNLILPPAW
                     AFGILYGGYTNQQETIKRVDSIMNHQYPIDAYWIDSWFWSHADKGIGPHKYIDFVADT
                     LGYPDRNKMWDYLQQNGIKGGFWAWDCILETGNEKAFTDFKQQGYFSSVYTNKNSWHN
                     KGTSTAMFQEKAEHPGTACGNIDFDNPAAVAYFKQRMKHFFAEGADFIKLDRTSKIST
                     CKAMFEMSQEFGLETKGRGFILSHTGGMETDEYKRYPTKWTDDTRSDWNIENPLIKFN
                     SWVPSVALKENIAMFTDPKNPTSRIPFLTNDLGGFDMGFTKKPAEELYIRWLQFSMFN
                     PITEVFSQPENPTANLAWNYSEKADSIFRFYAQLRMKLFPYIYSYAHRSRLEGKPMIG
                     KLPGQLYEYMFGNEMLVAPVYEKGSVSRKIYLPEGQWINYWTGEKLTGKKEYQVNAPE
                     EQLPLFIKEGSIIPSRRYAASIEKGNNDTLTLDIYPGKRGRFRLVEDDGLSNEYLKGI
                     FATTTIELRNSTLRINPTEGNYNGQSSSRNWIIQFHSAKKPKKVLLNGQVCTYDFNPE
                     QKLITITTGEKTKNKKWKIRLIP"
     gene            complement(3712..4614)
                     /locus_tag="PLU11_11360"
     CDS             complement(3712..4614)
                     /locus_tag="PLU11_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN59767.1"
                     /translation="MKKNWLILLIILVLSTGLANSQAADTTYKAVEYPEGYSAKLNVV
                     YATVKDWEGKMDLYLAPREKGLSPVVINIHGGGWNHGVKESQTGFSTFFKAGFAVANI
                     EYRLTGQATAPAAVEDTRCVLIYLIKNAKELNIDVNKIVIMGGSSGGHLALMGGLLGN
                     DHRFDGNCPGVENIKVAAIIDKYGITDVWDWGYGKILTSKSARNWLGDKATDPVFAAS
                     VSPITYVTKNSPPVFIVHGDADPTVPYQQSVDLHKKLKEAGVKTEFITVPGGLHGKFE
                     KTVNSDLNKAIMKFITEVFNNPKM"
BASE COUNT         1412 a         1102 c          985 g         1184 t
ORIGIN      
        1 gcaagcgcaa cagtaccgta ttatcgtata attgataccc gttcttttta gcaatcgtaa
       61 ctaaaattaa gaaataaccc tgatcatcac aaaatatgtt ctgaccaggg ttgaaaaaac
      121 gatatgattt ttatcaggtt tcctggtcaa ttacaggcta tttcattgcc acttgcagcc
      181 actccatgaa aaaaaataat aaaaaagtaa tatgccattt tatacttcac acaaaaaccg
      241 cgatgcggat atgaatctgt tgtagttttg aatgacaaaa tgattgcctt tgaaaaaaaa
      301 agcatacaat cagctgatcg tactttttct gccaatcctg gtcatcgcac agcaaaatga
      361 accggcatgg gatctcacag caaataaaag ctggcccctc ccttttcaac aagtacaaat
      421 caaatccacc atggataacc acttgcagca ggcctggtat tacgagagta ccggtaaccc
      481 gcttcaaccc ctgattatat ccctgcacac ctggagcggc gattacaacc aggaagaccc
      541 actggcccgg gaagcgttgc tcagaaactg gaattatatt catccggatt tcaggggtgc
      601 caataacaag ccggaagcct gtggcagccc cctcgtgatt gctgatatag aagatgccat
      661 ccggtttgcc attcaaaaag ggaaggtgga tacccataat atccatatca tcggtgtgtc
      721 cggcggagga tatactgccc tgctggccta tatgaaaatc cgctacccgg ttaaaagctt
      781 ttctgcctgg gtacccatca gcgacctgcg cagctggtac tgggaatcca ggggaagaaa
      841 agccaagtat gcagacgatg ttgaaaaagt ggccatgaaa aacggggcaa tggattggcc
      901 tgacctggac agccgctcac cgattcacct tctctaccct gcctctctcc gaaaaaatgc
      961 ggagctgcat atatatgccg gtgtccatga cggctacacc ggttccgtac ctatcagcca
     1021 gtctatgcag ttctacaata aaatcgctgc tgccatttat ccaacccgaa agcatttatt
     1081 aattactgaa acactgcaac agtctctcct ggctaaacga ataaacccgg cagctgatag
     1141 tacattaaaa ctgaacgacc ggcaaatcca cttgttcaga aaaacagacc ggatcagcat
     1201 cacccttttc gaaggaggac acgaaatgct ggtcccttcc gccctgacac tgatccctgc
     1261 ggataataat aaaaacaacg gcaacctgaa tattttggct attggcgatt caaacggggc
     1321 ggcggcggat ggatggccaa accaattgca gaaattactc ccctattcca ccataattaa
     1381 tacctgtgtt tccggcaata cgatcgggtt tgataatctc ggacaggaaa aactaaacac
     1441 ccttaaaaat ataacccgct acctggaaga tacctatcgc cagctgggac cggaaggtca
     1501 gctggattat attctgatcg gactgggtac caatgacaca aaagccatat ttaaagaaca
     1561 acaaaaagaa gtaccggcta atttatcaaa actgcttgat tcaatcaacg cttttatcca
     1621 tacccgaaaa ctcaaagcgc cggcaatctg tttattatta cctccgccgg tagatgaaca
     1681 aaaagcggat tcagttaaat acgggggatc agcggatcgg cttcttttaa acaatcccct
     1741 tttaaaaaaa gtagccgata cccataaagc tgccactata gatctgtatc aattgctcag
     1801 ttcaacaacc agtgaactga caacggatgg catccatctc actgaaatgg cacagtataa
     1861 agtggccggc tttattgttc accgattaaa taccagtcac aaaaaatgag aacatttatt
     1921 tcaacctgga cacttttgtt gctgattacc tttccctgct cccatacctg gtcgcaacaa
     1981 aataaaaaca gccggtatat tgtcgctgac accaacctga tcttaccccc agcctgggca
     2041 tttggtatac tgtatggtgg ctataccaat caacaggaga ccataaaacg ggtggattca
     2101 atcatgaatc accaatatcc aattgacgct tattggatag actcgtggtt ctggagtcat
     2161 gcggacaaag gcattggtcc gcataaatac atcgactttg ttgcggacac ccttggatat
     2221 cctgaccgga ataaaatgtg ggattacctg caacaaaatg gcatcaaagg aggcttctgg
     2281 gcctgggatt gcattcttga aaccggaaat gaaaaagcgt ttactgattt taagcagcag
     2341 ggatatttct cttccgttta taccaataaa aacagctggc ataataaggg taccagcacg
     2401 gccatgttcc aggaaaaagc agagcacccg ggtactgcct gcggcaatat agattttgat
     2461 aaccccgctg cagttgccta tttcaaacaa cgcatgaaac atttttttgc agaaggagct
     2521 gattttatca aactggaccg tacatcaaaa atcagtactt gcaaagccat gtttgaaatg
     2581 agccaggaat tcggactgga gacaaaaggc cggggtttca ttttatccca taccggtgga
     2641 atggaaactg atgaatacaa acgctatccc accaagtgga cagacgatac ccggagtgac
     2701 tggaatattg aaaacccatt gataaaattc aactcctggg ttccttcggt ggccttaaaa
     2761 gagaatattg cgatgtttac agatccgaaa aatccaacca gccggatccc cttcctgacc
     2821 aatgacctgg gaggatttga tatgggcttt accaaaaaac cagctgaaga gttatatatc
     2881 agatggctgc agttctctat gtttaatccg atcaccgaag tgttttccca acctgaaaac
     2941 ccaaccgcca atctggcatg gaattattcc gaaaaagcag atagtatatt ccggttctat
     3001 gcacagttgc ggatgaagtt gttcccatat atctacagtt atgcgcatcg gagccggctg
     3061 gaaggcaaac ctatgatcgg taaactgccc ggacaattgt atgaatatat gtttggcaat
     3121 gaaatgttgg tggcacctgt gtatgagaaa ggatcggtca gccggaaaat ttatttgccg
     3181 gaagggcaat ggataaacta ttggaccggg gagaaactta ccggaaaaaa ggaataccag
     3241 gtgaatgctc ctgaagaaca gctccctctt ttcataaaag aaggttccat tatcccctcc
     3301 cgacgttatg ctgcatccat tgaaaaagga aataatgata cgttgacact ggacatttat
     3361 cccgggaaaa gaggccggtt ccggttagtg gaagacgatg gtctgagcaa tgagtacctg
     3421 aaagggattt tcgccacaac aactattgaa ctgagaaatt caaccctgcg tataaatcca
     3481 actgaaggga attacaacgg ccagtcatcg agcagaaact ggatcatcca atttcattct
     3541 gctaaaaagc cgaagaaagt attattgaat gggcaggtgt gtacatacga tttcaaccct
     3601 gaacagaaac ttataacgat tactacagga gaaaaaacaa aaaataaaaa gtggaagatc
     3661 cggttgattc cttaaccgga cattccactt tttaaacata gtatattcaa cctacatttt
     3721 cgggttattg aaaacttcgg tgataaattt catgattgct ttattcagat cgctgttaac
     3781 tgttttctca aattttccat gtaaccctcc gggcacggta ataaattcgg tcttcacccc
     3841 tgcttctttt aatttcttat gcaggtccac cgactgctga taaggcacgg tagggtctgc
     3901 atcgccatgg acgataaaaa caggcggact gttcttcgtt acataagtga tcggtgaaac
     3961 agaagcggca aaaacggggt cagtcgcctt atcgccaagc cagttacggg ccgacttgct
     4021 ggtcaggatt ttcccatatc cccaatccca tacatcggtg atcccatact tatcaataat
     4081 ggcggccacc tttatattct ccacgcccgg gcagttgccg tcaaaccggt gatcgttgcc
     4141 gagtaaaccg cccattaaag cgagatgccc tccggaactg ccgcccatga tcactatttt
     4201 gttcacatca atattgagtt ctttggcatt tttaatcagg tagatcaaga cacagcgggt
     4261 atcttccacc gctgcgggtg ccgtggcctg accagtcagc cggtactcga tattggctac
     4321 tgcgaaaccg gctttaaaaa aagtactgaa accggtctgc gattctttaa ccccatggtt
     4381 ccagccaccg ccgtgtatat tgatcactac cggggatagt cctttttccc ggggcgccag
     4441 gtagagatcc attttccctt cccagtcttt caccgttgca taaacgacat tcaattttgc
     4501 actataccct tcgggatatt ccaccgcttt ataggtggta tccgccgctt gtgagttggc
     4561 cagaccggtt gacaaaacca gtattatcag gagaataagc cagttttttt tcatatactg
     4621 ttattgatat taataaccag ggttctgcgg aatgaccagt tgtgaaaaaa aactggctta
     4681 ttc
//
LOCUS       DAOPMW010000257         4679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21392_length_4679_cov_4.811851, whole genome shotgun sequence.
ACCESSION   DAOPMW010000257 DAOPMW010000000
VERSION     DAOPMW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21392_length_4679_cov_4.811851"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PLU11_11365"
     CDS             complement(<1..1580)
                     /locus_tag="PLU11_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN59768.1"
                     /translation="MPDYKEKYESLVRLASDGCIIYDPGGKILEFNEGACRHLGYTPE
                     EFKQLALFDLFLEEDLRKRPLRFDLMKNGDTALDYRKIKRKNGEAYLIELNSLMLPDG
                     NIMALATDVTENAKIRKELRMKEHAIASSISGMGITDLEGKIIYVNRALCDMWGALSE
                     PDLMGRNVQEMFEGTQVFDILPGLQDKAIEYGECTGKRLNGTIFPVAYSANIVPDKNN
                     NPAYIFGSFLDLSEQKKTRYLSDSIIDNLPVIFFMYDVPSGTIRRWNTRMEELTGYTR
                     AEISDMKYFELVYEQDRELAARKLLETVQLGKCDAEISLVDKQGKLHPFYFTGFLVKL
                     GDKEVVIANGIDISERKLSEEKLQRKYDQLQLIANLSDAVSKANQLDDIYKLALDGLL
                     TTIQADRASVLQFDEEGVMQFKAAHGLSEHYRLNAAGHSPWKQDAINPIPIFVTDVSE
                     EPSLSGLLPVIQNEGITALGFIPLVYMNHLLGKFMVYFNKAHQFTEEESQLLQTIARE
                     VAFAIGEKRNEIALRKSE"
     gene            complement(1878..2126)
                     /locus_tag="PLU11_11370"
     CDS             complement(1878..2126)
                     /locus_tag="PLU11_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59769.1"
                     /translation="MKDYNRFQVSPNPAPLNASITVLFPTDPVLAESYQITDDSGHLI
                     RRGKITERDRELFLSTGGMRDGVYWLVVGESRARFTIL"
     gene            complement(2289..>4679)
                     /locus_tag="PLU11_11375"
     CDS             complement(2289..>4679)
                     /locus_tag="PLU11_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN59770.1"
                     /translation="NRLRVNIGGMLAWLFIFSVSIAVIMLSENKKVEWEKRKRMADKM
                     AQSTDRSSEMLMSIAIQYLDADFLRENFNRFGSPESGKKIRDSIIADNYRGYLNRYDT
                     RLYVYDELNRPLFNEDATTYESLNTILTVQAKETEVEGLYYYETDLDKYNYITRRDIK
                     DTSNNRVGTFFIVSNPKIYSNEALFPELFRQFKQMDPENSPTYSIGVYHGGRLISPPG
                     NYSFPIWLKPEELPRREFEQRVNGDFNELWYKSGNEKVVVIARKKETLIQAITLFSYI
                     FCSFLFIVAVVQLLGFVFRTWLNGISLRRMFQLTIRKQIHSTFIFISVFSFVVIFFAT
                     VSSFISRYEQNNGEKLSRTMKIMVNEMQKKMADHTTFDDVIKIYDSVSNTGLQALVDE
                     VSDIHGVDVNVYDLNGNLQVSSEGNVYNKGVLSKKMDPMAYYNLEKLRKVLYLQKEKV
                     GTFSFYSMYSPVRDADGQAYAYINIPYFTSQPELEKEISNFLVTIINLNAFIFLIAGL
                     IAVFITNRITDSFSLISDKMKEVNLGKMNEAINWNRNDEIGELVQEYNTMVAKLGASA
                     TALAKSEREGAWREMARQVAHEIKNPLTPMKLSIQYLQKAINNNQENVKELSANVANT
                     LVEQIDHLSKIAADFSQFANIGNINTTEFDLHEVIFSLKELYAANEKVSFAWNAAAGP
                     VRLQADKTQMNRLFTNLFANAVEACSEKSHCHIIVNERLGKDTILVSIRDNGEGIPPE
                     MQERIFVPNFTTKSSGTGLGLAMCKGIVEQAKGRIWFETEQGSGTVFHVELPTLTS"
BASE COUNT         1252 a         1120 c          967 g         1340 t
ORIGIN      
        1 tgttcacttt tacgaagggc tatttcattt cgtttttcac caatggcaaa agccacttca
       61 cgggcaatag tctggagtaa ctgcgattcc tcttccgtaa actggtgagc cttgttaaag
      121 tagaccatga atttacccag gagatggttc atatagacca gcggtataaa accaagtgcg
      181 gtaatcccct cattctgaat caccggcagc agtccgctca aagaaggctc ttcagacaca
      241 tctgttacaa agattggaat cggattaatg gcgtcttgtt tccagggaga gtgccccgcc
      301 gcattcaacc ggtaatgttc cgataatcca tgcgcggcct tgaactgcat tacaccttct
      361 tcatcaaact gcaacacaga tgcccgatca gcctggatag tggttaacag cccatcgagt
      421 gccagcttat aaatatcatc cagctgattg gccttgctta cggcatccga tagattggcg
      481 atcagttgta actggtcata tttgcgttga agtttttctt cagataattt tctttctgaa
      541 atatcaatcc cattcgcaat caccacttct ttatccccca gttttaccag gaacccggta
      601 aagtagaagg gatgaagttt gccctgttta tccactaatg aaatttccgc atcgcacttc
      661 cctagttgaa ccgtttcgag cagcttccgg gctgccagct cccggtcttg ctcatagacc
      721 agttcaaaat atttcatgtc tgatatctct gcacgggtat aaccagtcaa ttcctccata
      781 cgggtattcc aacgcctgat ggttccggag ggcacatcat acataaaaaa gatgactggt
      841 aaattatcaa tgatgctgtc tgataaataa cgcgtctttt tttgttccga cagatcgagg
      901 aaagagccaa aaatataagc gggattatta tttttatccg gtactatatt ggctgaataa
      961 gcgacgggga aaatggttcc attgagtctt ttacctgtac attccccgta ttcaatggcc
     1021 ttatcctgta aaccgggcag tatatcaaat acctgggtac cttcaaacat ctcctgtaca
     1081 ttccgcccca ttaaatccgg ttcacttaag gccccccaca tatcacaaag cgccctgttt
     1141 acataaatga ttttgccttc caggtcggta atccccattc cactgatgga actggcaatc
     1201 gcatgttctt tcatgcgtaa ttccttacga atcttggcat tttcagtaac atcagtagcc
     1261 agtgccatga tattgccgtc aggcagcatc agggagttca gttcaatcag gtaggcctcc
     1321 ccgtttttcc gcttgatctt ccggtaatcg agggctgtat ccccgttttt catcaggtca
     1381 aaacgaaggg gtcgtttccg gagatcttct tccagaaaca aatcaaacaa agcaagttgt
     1441 ttaaattctt caggggtata gccaagatgc ctgcaggccc cttcattaaa ttccaggatt
     1501 tttccaccgg gatcataaat gatacaaccg tctgaggcaa gcctgacaag gctttcatat
     1561 ttttctttat aatcaggcat actaatcaaa atacctcttc cagttaagaa gttgaataca
     1621 cataaagata ataaaattag taagtgattt ttaaaggctg gtctttttgg cgtccttttt
     1681 cagaatatca gtgtttaccc ttgccgggag gaatgggtaa gtaagcaaaa tagaaaatga
     1741 gcagtaatcc tggcagcttt aggatccgca cagaagggat aaccgctttt tataagataa
     1801 cgacccgctg ccggatatat ttccagctgt ggatactaaa caaccggatg aaacccggga
     1861 gtcctgtttg attcaaatca tagaatcgtg aatcttgctc tgctctctcc taccaccaac
     1921 caatatacac catccctcat acctcccgtg ctcaggaaca attcccggtc gcgttcggta
     1981 atctttcccc gcctgatcag gtgaccggaa tcatctgtga tctggtagga ttcagcaaga
     2041 acaggatcag ttggaaacag cacagtaata ctggcattta aaggggccgg attaggactc
     2101 acctggaaac gattgtagtc tttcatgtaa tcagtgtctt aaccgggctg gtgaggcccg
     2161 aaaggatata tggtgctgtt ataaaaggaa caaaaaaaat gccatggatc aggcattgtt
     2221 aattacaccc ttcaaatgtg tatatcttca gaagttattt aagtagtcct gctgattacc
     2281 ctgcagaatt acgaagtaag agtgggcaat tccacatgaa agaccgttcc ggatccctgt
     2341 tcggtttcaa accagatacg tcccttggcc tgttccacaa tccctttaca catggccagt
     2401 ccaagtccgg ttccggatga tttagtggta aaattgggca caaagatgcg ctcctgcatt
     2461 tccgggggta tgccctcacc attgtcacgg atactcacca gtatggtatc ctttcctaac
     2521 cgctcattga cgatgatatg gcaatggctt ttctcactgc aggcttccac agcattggca
     2581 aacaggttgg taaataaacg gttcatctgg gttttatcgg cttgcagcct taccggtccc
     2641 gctgcggcat tccaggcaaa acttactttt tcattagcgg catagagttc tttcagtgaa
     2701 aatatcactt catgcagatc aaattccgtg gtattgatat taccaatatt ggcaaactgg
     2761 gaaaaatcag ctgcaatttt tgacaaatga tcaatctgct ccaccagggt attggctaca
     2821 ttggcagaaa gttcttttac gttttcctgg ttattattga tggctttctg caggtactga
     2881 atacttagtt tcattggggt cagcggattc ttgatctcat gagccacctg ccgggccatt
     2941 tcccgccagg ccccttctct ttcacttttg gctaaagccg tggcactggc acccagtttg
     3001 gccaccatcg tattatattc ctgaaccagt tcccctatct cgtcgttccg gttccagttg
     3061 atggcctcat tcatcttacc caggtttact tctttcatct tgtcactgat caatgaaaat
     3121 gaatcggtga tccggttagt gataaatacg gcaatcagtc cggcaatcag gaaaatgaag
     3181 gcattcagat taatgatcgt aaccaggaag ttggaaattt ctttctccag ttccggttgc
     3241 gaggtgaaat aaggaatatt tatataggca taggcctgtc catccgcatc cctgacgggg
     3301 ctgtacatac tgtaaaaaga aaaagttcct accttctctt tttgcaggta cagtactttc
     3361 cttagctttt ccaggttata ataggccatg ggatccatct tcttactgag caccccttta
     3421 ttataaacat taccttcgga agatacctgc aggttgccgt tcaggtcata cacattcaca
     3481 tccacgccat gaatatctga cacttcatcc accaatgcct ggagaccggt attagaaaca
     3541 gagtcataga tttttatgac atcatcaaag gtggtatggt ccgccatctt tttctgcatt
     3601 tcgttaacca tgatcttcat ggtacggctg agtttctccc cgttattctg ctcataccgg
     3661 ctgataaagg aggagacggt ggcaaagaag atcaccacaa aggaaaacac actgataaat
     3721 ataaacgtac tgtgaatctg tttacggatc gtcagttgaa acatccggcg gagactgatt
     3781 ccgttgagcc aggtacgaaa gacaaagccc agcagctgga caacggccac gatgaacagg
     3841 aaagaacaga aaatataaga gaacagggta atggcctgta taagtgtttc tttcttacgg
     3901 gcgatgacca ccactttttc attccctgac ttgtaccaga gttcattaaa atccccgttg
     3961 actctttgtt caaattccct ccggggcagc tcttccggtt tcagccagat aggaaatgaa
     4021 taattaccag ggggtgagat cagccggcct ccgtggtaaa caccaatcga ataagtgggg
     4081 gaattttccg gatccatctg cttaaattgc cggaacaatt cagggaacag cgcttcatta
     4141 ctgtaaatct ttggattaga tactatgaaa aaagtaccca cccggttgtt actggtatcc
     4201 tttatatccc gccgggtgat ataattatac ttatccagat ccgtttcata ataatataat
     4261 ccctccacct ccgtttcctt tgcctggacg gtcaggatgg tattcagtga ttcataggta
     4321 gtcgcatctt cgttgaacag cggccggttc aattcatcgt atacatataa acgggtatca
     4381 taccggttca ggtatccccg gtagttgtca gcgattatac tgtcacggat tttcttaccg
     4441 ctttccggac tgccaaaccg gttaaaattt tcccttagaa aatcggcatc caggtactga
     4501 atcgcaatgc tcatcagcat ttcactggag cggtcggtac tctgtgccat tttatctgcc
     4561 atccgcttcc gcttttccca ctccactttc ttattttcag acagcataat cactgcaatc
     4621 gatacagaga aaataaagag ccaggccagc ataccgccga tattcacccg gagccggtt
//
LOCUS       DAOPMW010000258         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21460_length_4668_cov_4.986126, whole genome shotgun sequence.
ACCESSION   DAOPMW010000258 DAOPMW010000000
VERSION     DAOPMW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21460_length_4668_cov_4.986126"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLU11_11380"
     CDS             <1..239
                     /locus_tag="PLU11_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HPN59771.1"
                     /translation="CNAQMSSKQLKEICVINSVGANLLKVAMQKLNLSARAYDRILKV
                     SRTIADLSDSPDIKPEHLAEAIHYRSLDREGWGG"
     gene            complement(286..1464)
                     /locus_tag="PLU11_11385"
     CDS             complement(286..1464)
                     /locus_tag="PLU11_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN59772.1"
                     /translation="MNTDKGNRRKFLQEVTKASGVMMLAPIAGNFTYVEPVKSSKMPV
                     IQPGKIKFAVININHPHIYGMTEAIKRGGGELVALYAKEADLTAAYLKAYPEARLAKS
                     ENEILEDPSIHLVLSSGIPDERAPLGIRVMQHGKDYLTDKPGIITLKQLADVKKVQQA
                     TKRIYSIMYSKRLENKGTEKASQLVKAGAIGQVIQTINLAPHRINNNIGKTGLAVRPD
                     WFWDKKRFGGILTDIGSHQFDQYLHFTGSTEAAVVASQVGNLHHLDKPLFEDFGDVML
                     RGNGGMGYIRVDWFTPDALDSWGDGRLTIMGTDGYIEVRKNTDILSGKKGGNHLYLVN
                     KKEMLHIDCNNEMLPFGPLFVDDVINRTETAMPQAHCFLATELALKAQKKATLLGLNN
                     "
     gene            1740..3590
                     /locus_tag="PLU11_11390"
     CDS             1740..3590
                     /locus_tag="PLU11_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN59773.1"
                     /translation="MKILFKYLRPYKWLVGLVLLLAAVNIGFSLVDPIIFGRLINLAT
                     YHQNKIAFSWDSFLFFNESITEEVIKVKPGGIVEKVMETRHYYGVAWLLLASITVAMI
                     SRIAKAFQDYFLNLITQKFGATVFTEGLQHAMKLPYQEFEDARSGETLSVLQKVRLDT
                     EKFVSSFINVLFPVVVGILFVAVYATSIHWSLPLVYFGGIFLLTIISNLLSKKVKTIQ
                     KKIVGQTTALAGATTESLRNIELVKSLGLTQQEVSRLNKNTFKILGLELTKVKRIRSI
                     SFIQGTFVNTLRQIILFLLMLFIYRGRMDAGQIVTMQIFSFFVFGPLQEIGNIILTYR
                     EAQASLENFANLMKKSPEPKATNPSHLGKIETLDFDHVAFKHQTASQNAINDISFKVK
                     TGETIAFVGPSGSGKTTLMKLLVGLYRPQDGKILYNGMDENSIDFEDLRNQIGFVTQD
                     TQLFSGSIRENLLFVNPAATEEDLEDVLNKASCQNLLVRAEKGLDTMIGEGGLKLSGG
                     EKQRLSIARALLRKPKLLLFDEATSALDSLTEEEISDTIRDISKEGNQITILIAHRLS
                     TIMHADRIYVLEKGDVVETGNHELLIAEKGLYYAMWRQQIGERKNRAAVV"
     gene            3600..3908
                     /locus_tag="PLU11_11395"
     CDS             3600..3908
                     /locus_tag="PLU11_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN59774.1"
                     /translation="MGIFSFFGNGSIKKALRQGAVVIDVRSASEYDRGRVPGSLNIPV
                     DRIGAGIPRIKGLNKPVICVCASGHRSRIASATLKQAGLKDVYNGGSWERVLRLIESL
                     "
     gene            complement(3909..4355)
                     /locus_tag="PLU11_11400"
     CDS             complement(3909..4355)
                     /locus_tag="PLU11_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59775.1"
                     /translation="MRNGIQFIFLSLFLTGLLMACSKSGSSTDDGGGGGGPHIVTDND
                     TTRPVLTVITPLSNQVYASGSSISVTGTITDDMGLYRGSIRITNDVTGEILKEQLYEI
                     HGIRSYNFNVPAIANVTAVTDYTVSVFFEDHGLNSISQTLKVKMNP"
     gene            complement(4373..>4668)
                     /locus_tag="PLU11_11405"
     CDS             complement(4373..>4668)
                     /locus_tag="PLU11_11405"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HPN59776.1"
                     /translation="WQIKNLRRRINLIRYMQGLGVFSFLGCVGCMYSLYRGAAVVAEF
                     IFAVALLSLLASLFISLAEIIQSTKAIELELSDIEELEKGNIFTDILSGTKEK"
BASE COUNT         1219 a         1084 c         1061 g         1304 t
ORIGIN      
        1 attgcaatgc acagatgagc agtaagcagc taaaagaaat ctgtgtgatt aattcggtgg
       61 gagccaatct tctcaaagta gccatgcaaa agctcaactt gtcagcccgg gcctatgacc
      121 ggatcctgaa agtcagccgg acaatcgctg acctgtctga cagtcccgat ataaaacccg
      181 aacacctggc agaagcgatc cattaccgga gtcttgaccg ggagggctgg ggcggataat
      241 tgatgacagc actttgtcag cgctgttcat atcatcaaag gtggattaat tgtttaatcc
      301 aaggagggtt gctttcttct gtgcttttaa tgccagttcg gtggcgagga aacaatgtgc
      361 ctgcggcata gccgtttctg tcctgtttat cacatcgtct acaaacaaag gaccaaaggg
      421 cagcatttca ttgttgcaat ctatgtgcag catttctttt ttattcacga ggtataaatg
      481 atttccgcct tttttcccag aaagtatatc cgtgtttttt cgtacttcta tatagccatc
      541 cgtgcccata atggttaacc tcccatcgcc ccaggagtcg agcgcatcgg gtgtaaacca
      601 gtcgacccgg atataaccca ttcctccgtt accgcgcagc atcacatcgc caaagtcctc
      661 aaacaatggt ttatccaggt ggtgcagatt tccaacctgt gacgccacca ctgctgcttc
      721 cgtactgccg gtaaaatgca ggtactggtc gaactgatgc gaaccaatat cagtcaggat
      781 accaccgaac cgtttcttat cccagaacca atccggacgt acagccagtc cggttttgcc
      841 tatattatta ttgatcctgt gtggcgctaa attgatcgtt tgaatcacct ggccaatagc
      901 ccctgcttta accagctgac tggctttctc tgtgcctttg ttttccagcc gtttgctgta
      961 catgatggag taaatccgtt tggtagcctg ttgtactttt tttacgtccg ccagttgctt
     1021 gagcgtaatg ataccgggtt tatccgttaa ataatccttg ccgtgctgca tgacccggat
     1081 acccaaggga gcccgttcat ccggtatccc tgaacttaac actaaatgaa tagaagggtc
     1141 ttccagtatt tcgttttcac tttttgccag tctggcctcc ggatacgctt ttaaataggc
     1201 ggccgtcagg tctgcttctt tggcatataa ggccaccagt tctcccccgc ctcttttaat
     1261 cgcttccgtc atcccataaa tatgcggatg gttgatattg atgacggcaa attttatttt
     1321 cccaggttga ataacaggca tcttgctgga tttgacaggc tctacatagg taaagttgcc
     1381 ggcgattggc gccaacatca tcactccaga cgcttttgtc acttcctgca aaaactttct
     1441 tctgttccct ttatctgtat tcatacctgt tatattattt ttttaatact aaagtgatca
     1501 tttgaaattc actaccggct gccaaccgga catacctcgt ataaaaatac tgaatctgac
     1561 tgaccggcta cctggcggcg gccattaata cccggactgc tgatcggaga gacaacctgt
     1621 ataaccggtt aattaacaga aatatacaac cagggacctg ttaacttggc atatttccta
     1681 aacttgtacg aagtttgtac attgaatctg catttccatt tttcgcctca atcccattta
     1741 tgaagatatt atttaaatac ctcaggccat ataaatggct agtaggactt gttttattac
     1801 ttgctgctgt gaatatcggg ttctccctgg tggaccccat cattttcggg cggctgatca
     1861 acctggctac ctatcatcag aataagattg ctttttcttg ggattcgttc ctgttcttca
     1921 acgaaagcat tacagaagaa gtgatcaagg taaaaccagg cgggatcgtt gaaaaagtca
     1981 tggaaacccg tcattattac ggggtggcct ggctcttgct ggcttccatt accgtggcga
     2041 tgatcagccg gattgccaaa gctttccagg actatttcct gaacctgatt acgcagaaat
     2101 ttggagctac cgtatttaca gaaggcttgc agcatgcgat gaaactcccc taccaggaat
     2161 ttgaagatgc ccggagcggg gaaaccttaa gtgttttgca gaaagtaaga ctggatacgg
     2221 aaaagtttgt ctcttcgttt atcaatgtac ttttcccggt agttgtgggt attttgtttg
     2281 tggcggttta tgccaccagt attcactgga gtttaccgct cgtttatttt ggaggtatct
     2341 tcctgctgac cattatttcc aacctgctga gtaaaaaggt aaagacgatt cagaaaaaga
     2401 tcgtgggaca aaccacagcg ctggccggtg ccactacgga atccctgcgt aatattgaac
     2461 tggtgaaaag cctcgggcta acccaacagg aagtatcacg actgaataaa aacaccttta
     2521 agatactcgg actggaactg accaaggtaa aacgtatccg cagtatcagt ttcatacagg
     2581 gtacatttgt caatacactt cgccagatca tattattcct gctgatgttg tttatctacc
     2641 ggggaagaat ggatgccgga cagattgtga cgatgcagat cttttccttt tttgtgtttg
     2701 gtccgttgca ggagattggc aatattattc tcacttatcg cgaggcccag gcttccctgg
     2761 agaattttgc caacctgatg aaaaaatcgc cggaacccaa agccactaat ccgagtcatt
     2821 tgggaaagat cgagaccctg gattttgatc atgtggcatt caagcaccag accgcttcac
     2881 agaatgcgat caacgatatc agcttcaaag taaaaaccgg ggagaccatt gcttttgtag
     2941 ggccttccgg ttccggtaaa accactttaa tgaaattact ggttggcttg tacaggccgc
     3001 aggatggaaa gatcctgtat aatggaatgg atgagaacag tattgacttt gaagacctgc
     3061 gcaaccagat cggttttgtg acccaggata cccagctttt ttccggctct atccgtgaga
     3121 atctgctgtt tgtgaatcca gctgcaactg aagaagacct tgaagatgta ttgaataaag
     3181 cctcctgtca gaatctgtta gtacgtgcgg agaaagggct ggataccatg atcggtgaag
     3241 gagggctcaa gttgtccggt ggagaaaaac aacgtttatc tattgcccgt gcgttgttac
     3301 gcaagcccaa attgttatta tttgacgaag ccacttcagc gcttgattca cttacggaag
     3361 aagaaatttc agacactatc cgcgatatat caaaagaagg caatcagatt acaattctga
     3421 tcgcccaccg gctgagtacc attatgcatg cggaccggat ctatgtactc gagaaaggcg
     3481 atgtggtgga gacggggaat catgaattac tgattgcgga gaagggatta tattatgcga
     3541 tgtggaggca gcagatcggg gaacgaaaaa acagggctgc tgtcgtttaa acttattgta
     3601 tgggaatttt ctcctttttt ggaaatggct ccatcaaaaa agccttaagg cagggagctg
     3661 tagttattga tgtccgatct gccagtgagt atgaccgtgg acgcgtcccc ggttcactga
     3721 atattccggt tgaccggatc ggtgcgggta taccacgtat taaagggctg aataaaccgg
     3781 tgatttgtgt ttgtgccagt ggtcaccgga gccggatcgc ttcagctact ttgaaacaag
     3841 ccggtttgaa ggacgtatat aatggaggaa gctgggagcg ggtgctccgg ctgattgaat
     3901 cgctctgatc agggattcat ttttacttta agggtctgac tgatactgtt cagcccgtgg
     3961 tcttcaaaaa atacactcac ggtataatct gtaacggcgg taacattggc gatggccggt
     4021 acattgaaat tataggatct tattccatgt atctcgtata gctgctcttt caggatttca
     4081 cccgtcacgt cattggtaat acggatactg ccccggtata agcccatatc atcggtaatt
     4141 gttcccgtaa cactgataga agaaccactc gcatatacct ggttactcag cggggtgatt
     4201 acggtaagca ccggccgggt ggtatcatta tccgtgacaa tatgcggacc gcctcctccc
     4261 ccaccatcat cggtactgct tccggatttc gaacaggcca tcaggagacc tgtcagaaaa
     4321 agtgataaga atataaactg tatcccgttc cgcatgtctc aaatatattg ttttattttt
     4381 ctttagtccc cgagaggata tccgtaaaaa tattgccttt ctccaactcc tcaatatcac
     4441 tcagttccag ttcaatagcc ttggtactct ggataatttc cgccagggaa ataaacaggg
     4501 aggccagcag gctcagcaac gccacagcaa aaataaactc tgctaccaca gccgctcccc
     4561 ggtataaact gtacatacaa ccaacacatc ccaggaaact gaataccccc agtccctgca
     4621 tataacggat caggttgatc cggcgacgca gatttttgat ctgccaga
//
LOCUS       DAOPMW010000259         4667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21465_length_4667_cov_8.033825, whole genome shotgun sequence.
ACCESSION   DAOPMW010000259 DAOPMW010000000
VERSION     DAOPMW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21465_length_4667_cov_8.033825"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLU11_11410"
     CDS             <1..576
                     /locus_tag="PLU11_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPN59777.1"
                     /translation="TMAKAKYTTENVGHYGLGFEHYCHFTSPIRRYPDVQVHRILFDV
                     LNNTMQPDKKMEEKCKHTSERERAAMQAERDSNKYKQVQYMRNYMGEEFDGVISGVAS
                     FGFWVETVDHKCEGLVSINSLLEYDEFRHVEAEYCLLGHRSAKTFRMGDKVRIKVVGA
                     NLGKRQLDYEWVVSGEFAEEKSTRQKGKKRK"
     gene            592..1557
                     /locus_tag="PLU11_11415"
     CDS             592..1557
                     /locus_tag="PLU11_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPN59778.1"
                     /translation="MQSFELLSQKFAFHFEKAHFPEAPSTLYQPNEYFLKLGGKRVRP
                     VLCLMGNELFDDIREDAWELATAVELFHNFTLIHDDIMDKAPLRRGMQTVHTKYGENT
                     ALLAGDVMLVKAYEYLSRIEGDFLKPIMSLFNQTAREVCEGQQWDMDYEKQETVHLDE
                     YLRMIELKTSVLLAASLKMGAILGGSGERNQNLLYEFGRKLGIAFQVQDDYLDAFGDP
                     EKFGKQVGGDILANKKTFLLIHALETANAGQLEELKQLIAGNPADKVEQVLRIFRDCK
                     VDEWAIELKDKYLDEAFQHLDDIAVLSKRKQPLTDLARFLVQREH"
     gene            1626..3332
                     /locus_tag="PLU11_11420"
     CDS             1626..3332
                     /locus_tag="PLU11_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN59779.1"
                     /translation="MSNSLFRKKSIDVIKKEYEAGLSDHSSGLKKILGVRDLTFMGIA
                     AVIGAGIFSTIGGAAYNGGPGISLLFVITAITCGFSALCYAEFASRVPVAGSAYTYSY
                     VTFGELIAWIIGWALILEYAIGNIVVAISWSGYFNNLLVHVFNINLPSWMLVDPVTAG
                     NAFTEASNAMAAGNIADPEKLKSYQFAIDAYNNAPVIGGNHIFFNLPAFIVVALITWL
                     AYIGIKESKQSANFMVIFKVAVIIFVIIAGAFFVDTNNWTPFMPNGFEGVLKGVSAVF
                     YAYIGFDAISTMAEECKDPQRDMPKGMIYSLIVCTVLYILIALVLTGIESYSSFKDVN
                     DPLAFVFEKRAPWIEKIVSISAVVATTSVLLVFQIGQPRIWMSMSRDGLLPKIFQKIH
                     PKFQTPSFATILTGVIVGVGALFIQSGLVTDLTSIGTLFAFVLVSGGVLLLPRVAKEP
                     GKFHLPYINGKFIVPALMALFIYVFRDRLQESLKHIGHENYQEVLFLVFVLIAVILSV
                     LTFIRNFSFIPIMGVLSCLYLMIEIPAVSWTWFFAWMGLGLLIYFMYGYRNSKLAGKV
                     RS"
     gene            3473..4507
                     /locus_tag="PLU11_11425"
     CDS             3473..4507
                     /locus_tag="PLU11_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPN59780.1"
                     /translation="MKYQIGDTVLILHSNEEAKVVDIINNKMLMVETKGVSYPVYMDQ
                     VDFPYFKRFSEKKLVPEKKEKKYIDDVRKEKYVESKRVEDGVWLTFLPVMDQDEFGDL
                     VVEELKLHLVNHTKTPYNFVYKLHFFGKPDFELKNTIQPFEDFYLHDIEFSELNDSPS
                     FDFEFTLVTPDKNLAPHFEAGVKLKPKQLFAKIEELKKKNLATFSQRLFETYPVRTYE
                     EVVESENAWKHQLSKAGYKIYDASKARQHLEPARSVVDLHIEKLTDHWQDMDNYEMLQ
                     LQLKNFEKYFHLAVAHHQPSLIIIHGVGEGRLRDEIHDLLRLKKEVKSFVNRHHPAYG
                     YGATEIFFQY"
BASE COUNT         1232 a         1030 c         1174 g         1231 t
ORIGIN      
        1 accatggcca aggcaaaata taccacggag aatgtggggc attatggcct gggctttgaa
       61 cattattgtc attttacttc acctatccgc cggtacccgg atgtgcaggt gcaccggatt
      121 ctgtttgatg tactgaacaa cacgatgcag cctgataaga agatggaaga aaaatgcaaa
      181 cataccagcg aacgcgaacg ggcggcgatg caggcagaac gggattccaa caagtacaaa
      241 caggtgcagt atatgcgcaa ttatatgggc gaggaatttg acggagtgat cagcggggtg
      301 gcctcatttg gattctgggt cgagaccgtg gatcacaaat gtgaaggact tgtcagcatc
      361 aacagcctgc tggaatacga tgaattcagg catgtggaag ccgaatactg cctgctgggt
      421 caccgcagtg ccaaaacctt ccggatgggt gataaggtcc ggatcaaagt ggtgggtgcc
      481 aatcttggta aacgtcaact ggattatgaa tgggtggttt ccggcgaatt cgccgaagaa
      541 aaaagcaccc ggcaaaaagg taaaaaacgt aaatgatccc tatattggcc tatgcaaagt
      601 tttgaattac tctcacagaa atttgccttt cattttgaaa aagcgcattt tccggaagcc
      661 ccgtccacac tctaccagcc caatgagtat tttctgaagc tgggggggaa gcgggtcaga
      721 ccggtgctct gccttatggg caatgaacta tttgacgata tccgggaaga tgcctgggaa
      781 ctggccaccg cggtagaact ttttcacaat ttcacgctca ttcatgatga tatcatggat
      841 aaggctccgc tgcgtcgcgg catgcagacg gtacatacga agtatgggga gaacactgcc
      901 ctccttgccg gagatgtgat gctggtgaaa gcctatgagt acctgagccg gattgaaggg
      961 gatttcctga agccgatcat gagtctgttt aatcagacgg cccgggaagt ctgtgaaggc
     1021 cagcaatggg atatggatta tgagaaacag gaaaccgtac acctggatga atatctgcgg
     1081 atgatagagt tgaaaacatc cgtattgctg gcggccagtc tcaaaatggg cgccattctt
     1141 ggcggctccg gggaacgcaa ccagaatctt ctatatgaat ttggccgtaa actcggcatt
     1201 gccttccagg tgcaggatga ttacctggat gcattcggcg atccggaaaa attcggtaag
     1261 caggtagggg gcgatattct cgctaataaa aaaaccttcc tgctgatcca tgccctggaa
     1321 accgccaatg ccgggcaact cgaagaactg aaacagttaa tcgccggtaa tcccgccgat
     1381 aaagtggagc aggtactccg gatattcagg gattgcaaag tagatgaatg ggctattgag
     1441 ttaaaagata aatacctgga cgaggccttc cagcacctcg atgatattgc ggtgctgtcg
     1501 aaacggaaac aacccctgac cgacctggcc aggttcctgg tacagcggga acattgattg
     1561 tttgccgtta ttccctgtta tgccatattt ttttcctatt ttgatcccac aaaaccaact
     1621 gactgatgtc aaattcatta ttccggaaaa aatctattga tgtcattaaa aaagaatatg
     1681 aagccggact ttccgatcat agttcaggac tgaaaaagat actgggtgtc agggacctca
     1741 cgtttatggg gattgccgcg gtcatcgggg ccggtatctt ttccactatt ggtggagccg
     1801 cttataatgg cggacccggc atctcacttt tatttgtaat caccgccatt acctgtggtt
     1861 tttcggccct ttgttatgca gaatttgcaa gccgtgttcc ggtggcgggc agtgcctata
     1921 cctattccta tgtaacattc ggggaactga ttgcctggat tattggctgg gccctgatcc
     1981 tggaatatgc catcggaaat attgtagtgg cgatttcctg gagtggctat tttaataacc
     2041 tgctggtaca tgtctttaat atcaatctgc cttcctggat gctggtggat ccggtaactg
     2101 cggggaatgc ttttacggaa gccagcaatg cgatggcggc cgggaatatc gcggatccgg
     2161 aaaaattgaa aagttatcag tttgctattg atgcgtataa taatgcaccg gttatcgggg
     2221 gtaatcatat cttctttaac ctgccggcct ttatcgtagt agccctgatt acctggctgg
     2281 cctatatcgg gatcaaggag agtaagcagt ccgctaattt catggtgatc ttcaaagtgg
     2341 ccgttatcat ttttgtgatt attgccggcg cattctttgt ggataccaac aactggacgc
     2401 cctttatgcc taatggattt gagggcgtac tgaaaggggt atccgccgtg ttctatgcct
     2461 atatcggatt tgatgccatt tccaccatgg ccgaggagtg caaggacccg caacgggata
     2521 tgcccaaggg gatgatctat tctttaattg tatgtaccgt attatatatc ctgatcgccc
     2581 tggtgctgac tggtattgaa agctattcat cctttaaaga tgtaaatgac ccgctggctt
     2641 ttgtttttga aaagagagcc ccgtggattg aaaaaattgt ttcaataagt gccgtggtag
     2701 ccactacatc tgtactgctg gttttccaga tcgggcagcc ccgtatctgg atgagcatga
     2761 gccgcgatgg tttattgcct aaaatatttc agaaaataca tccgaaattc cagaccccct
     2821 ctttcgctac catcctgacc ggagtgatcg tgggagttgg cgctttgttt atccagagcg
     2881 gactcgtaac cgatctgacc agtatcggga ccttatttgc tttcgtgctg gtatccggcg
     2941 gtgtcctgct attaccacgg gtggctaaag agccgggtaa atttcatctg ccttatatca
     3001 atggcaaatt tatagttccg gccctgatgg ccctgtttat ctatgtcttc cgcgaccgct
     3061 tacaggaatc cttaaaacat atcggacatg agaattacca ggaagttctc tttctggtat
     3121 ttgtattaat cgcagtaatc ctgtctgtat tgacattcat ccgcaatttt tctttcatcc
     3181 ccatcatggg cgtactatcc tgtctctacc tgatgattga aatcccggcc gtcagctgga
     3241 cctggttctt tgcctggatg ggtctggggc tgctgattta ttttatgtac gggtacagga
     3301 atagtaagct agcgggtaaa gtacgaagtt agaagtacga agtacgaaag tagtccgtaa
     3361 aagtgattga gcctgttaga attgattgta aatgctaaga tggtactttc gtacctcgca
     3421 cctcgtactt cgtacctgtg ccttatcttt gctttctttt gaaaaccccg caatgaagta
     3481 ccagatcggc gatactgtcc tcatcctcca ttccaacgaa gaggccaaag tggtggatat
     3541 catcaacaac aaaatgctga tggtggagac caagggcgtg agctatccgg tgtatatgga
     3601 tcaggtggat ttcccgtatt ttaaacgatt ttctgaaaag aagctggtgc cggagaaaaa
     3661 ggaaaagaaa tatattgatg atgtgcggaa ggagaaatat gttgaatcca agcgggtgga
     3721 agacggggtg tggctgacct ttctgccggt aatggatcag gatgaatttg gcgacctggt
     3781 ggtggaggaa ctgaaactgc atctggtaaa tcataccaaa accccgtaca attttgtata
     3841 taagctgcat tttttcggca aacccgattt tgaactgaag aataccattc agccctttga
     3901 agatttttac ctgcacgata tcgaattcag tgaattgaat gacagtccga gttttgattt
     3961 tgaatttacc ctcgttactc cggataaaaa cctggccccg cattttgaag ccggggtgaa
     4021 actcaaaccc aaacagcttt ttgctaaaat cgaagagctg aagaaaaaga acctggccac
     4081 tttttcccag cgtttatttg agacctaccc ggtccgtacc tatgaagaag tggtggaatc
     4141 ggaaaatgcc tggaaacacc agttgtcaaa agccgggtat aaaatatatg atgcctccaa
     4201 agcccggcaa cacctggaac cggcgcgttc ggtcgtagac ctgcacatcg aaaaactcac
     4261 agatcactgg caggatatgg ataattatga aatgctccag ttgcaactga agaatttcga
     4321 aaaatacttt catctcgccg tggctcatca ccagccctcc ctgattatca ttcacggggt
     4381 aggggaaggc cgtctccgtg atgaaataca tgacctgctc cggctgaaga aggaagtaaa
     4441 gtcctttgtc aaccgccacc atccggccta tgggtacgga gctactgaaa tattcttcca
     4501 atactgattt tcctttctac ctttgaacag ctacaagcca ggctatcgct gcctgtcatc
     4561 ctgagcgcgt cgaaggagac aggtggagga aagtccggac agcacagagc aatgcaccgg
     4621 tgaagagccg gcctgttgaa agacagagag atagtgccac agaaaat
//
LOCUS       DAOPMW010000260         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21471_length_4666_cov_6.579050, whole genome shotgun sequence.
ACCESSION   DAOPMW010000260 DAOPMW010000000
VERSION     DAOPMW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21471_length_4666_cov_6.579050"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1944
                     /gene="rpsA"
                     /locus_tag="PLU11_11430"
     CDS             16..1944
                     /gene="rpsA"
                     /locus_tag="PLU11_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPN59781.1"
                     /translation="MRETNIMFENNIKKQLNADVQESGAEETVAAATTTAPVVAAATA
                     HDDFDWSVDKRNVTSYTKEEKEKYDKVYENTFVQLNDGELMKGLVVGLTKTDVVLNIG
                     FKSDGLISLNEFRDIPNLSIGDEVEVMIVEKEDREGHLNLSRRQARITRAWERIVEVH
                     KTGEVITGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKIN
                     ETIKNAVVSHKALIESDIEAQRAEIMSKLEKGQVLEGTVKNITDFGAFMDLGGLDGLL
                     YITDISWGRISHPSEVLKLDQKVNVVVLDFDDEKKRISLGLKQLTPHPWDVLGENIVE
                     GNVVKGKVVNIEDYGAFLEIQPGVEGLVHVSEITWANTPINAKEFFKLGDEHEAKIVT
                     LDKDSRKMSLSIKQLSADPWNDIETKFAEGSRHTGLVKNITNYGVFVELAPGIGGMIH
                     ISDLSWLKRFNHPSEYTKVASNIEVIILGIDKDNRKLQLGHKQLEEDPWNTLQDTFAI
                     GSIHEGTVTRRDDKGATVQLPYGLEGFAPNRHLNKEDGKSIGADETNQFMVIEFDRNE
                     KRIVVSHTRIWEQGKVEEVESAKKEARAEADKTKKAVKTIQSKVEKATLGDLGVLADL
                     KKKMEGNTEEGAAEAKPE"
     gene            2204..2938
                     /locus_tag="PLU11_11435"
     CDS             2204..2938
                     /locus_tag="PLU11_11435"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59782.1"
                     /translation="MNTLMSRMYFVLFNFIRPYSLLFFILFVSLQVRGSGHSAAHIHS
                     YTSQQLYKVDTGGGILPLHNFTLTVTLKENTVFLKWVAENEMNTEKFVIQRSTDGNSF
                     KNMGELPPTGPINILTEYNSADDIQGIVSNVAYYRIKAEDNRGNFAYSNVVPVRLTKA
                     DGFSFWPNPFSTSLNLSYNASLSTSIRVEMYDNSGRRVIQQLFNVNRGMNQLSVNGAA
                     GLSHGLYHVRIIDLLTNEVSFSKMAK"
     gene            3027..3794
                     /locus_tag="PLU11_11440"
     CDS             3027..3794
                     /locus_tag="PLU11_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN59783.1"
                     /translation="MAAILNRLKYFNPVRKLVYMIVGAASYPGLAIINKLSISGTEHL
                     KGLPREKVLFVSNHQTYFADVITFLQIFCAVKWGKMNRLGVPYYLLNPFTRVNYVAAE
                     ETMKGSLISRLFLLAGGLTVKRTWRAEGKEVRRGLDPSDTRKITRALERNWVITFPQG
                     TTKPFAPGRKGTALIIKQVKPIVIPVVINGFWRAFNKKGLKFKKKGSLLSVTFKAPLE
                     IDYEAPADVILAQLMDAIEQSKKFMMMGKHHWQSTDK"
     gene            complement(3795..4310)
                     /locus_tag="PLU11_11445"
     CDS             complement(3795..4310)
                     /locus_tag="PLU11_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPN59784.1"
                     /translation="MDARKASASLTIMTEIVLPNDTNVFGNLMGGRLMYWMDIAAALA
                     SQKHCNAPVVTASVDNISFENPIKLGNAVHIEAKVTRAFNTSMEIHMKVWGEDMTQQF
                     RYKSNEAYYTFVALDPNRKPRPVPAVIAENEEEKKLFEGALRRRQLRLILGGKMKPDD
                     ALELKAFFMKD"
     gene            complement(4363..>4666)
                     /locus_tag="PLU11_11450"
     CDS             complement(4363..>4666)
                     /locus_tag="PLU11_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59785.1"
                     /translation="SIPIFLSGIPYKLFTRQQIQMPFVLPGDVTAHTNFPAPMEMFSD
                     SYNWVMVPNVYGMTQFADGGLMTTKPYISGSNYLIKMGAGKILQKALPQQIEPVPG"
BASE COUNT         1338 a         1019 c         1099 g         1210 t
ORIGIN      
        1 ccccgaaata aaaccattcg ggaaacaaat atcatgtttg aaaacaacat taaaaaacaa
       61 ttaaacgctg atgtacagga gtccggtgcc gaagagaccg tggctgcagc gacaacaaca
      121 gcacctgtgg tagccgcggc tactgcccac gacgatttcg actggagcgt ggacaaacgt
      181 aatgtcactt cttataccaa agaggaaaaa gagaagtacg acaaagtgta cgaaaataca
      241 tttgtacagc tgaatgatgg cgaactgatg aaaggtctgg ttgttggtct gaccaaaacg
      301 gatgtggtgc tgaacatcgg attcaagagt gatggtctta tttcactcaa cgaattccgt
      361 gacatcccca acctgtcaat cggtgatgaa gtggaagtca tgatcgtgga gaaagaagac
      421 cgggaaggtc atctgaacct cagccgccgc caggcacgta ttacccgtgc atgggagcgt
      481 atcgttgaag tacacaaaac aggtgaagta atcaccggta ccgtgacttc taaaaccaag
      541 ggtggtctga tcgtggatgt gtttggaatg gaaacattct tacccggatc acagatcgac
      601 gttaaaccgg ttaccgatta cgatcagttt gtaggaaaga ccatggaatt caaagtggtg
      661 aagatcaacg aaaccatcaa gaacgccgta gtttctcaca aagccctgat cgaaagtgat
      721 atcgaagcac aacgtgcaga gatcatgagc aaactggaga aaggccaggt gctcgaagga
      781 actgtgaaga atatcaccga cttcggtgca ttcatggacc tgggcggact cgatggtctg
      841 ctgtatatca cggatatttc atggggccgt atctctcacc cgtctgaggt gctgaaactt
      901 gaccagaagg tcaatgtggt ggtacttgat ttcgatgatg agaaaaaacg tatcagcctc
      961 ggtctgaaac aactgactcc gcatccttgg gatgtactgg gtgagaacat tgttgaaggc
     1021 aatgtagtaa aaggtaaagt ggtgaatatt gaagattacg gagctttcct ggaaattcaa
     1081 ccgggtgttg aagggctggt gcacgtaagc gaaatcacct gggccaatac cccgatcaat
     1141 gccaaagagt tcttcaaatt aggtgatgag catgaagcaa agatcgttac cctggataaa
     1201 gacagccgca agatgagtct ctctatcaag cagctttctg ctgatccatg gaatgatatc
     1261 gaaacaaaat ttgctgaggg cagccgtcat accggcctgg tgaaaaacat caccaattac
     1321 ggtgtgtttg tggaactggc tcctggtatc ggtggtatga tccatatcag cgacctgagc
     1381 tggctgaaac gctttaatca cccgagtgaa tacacgaaag tggcttcaaa cattgaagta
     1441 atcattctcg gtattgataa agacaaccgc aaactgcaac tcggacacaa acaactggaa
     1501 gaagatccct ggaatacgct gcaggatacc tttgcaatcg gcagcattca tgaaggaacc
     1561 gtaacccgcc gcgatgataa aggcgccact gtacagctgc cttatggcct ggaaggtttt
     1621 gcccctaacc gtcacctgaa caaggaagat ggtaagagca tcggtgcgga tgaaaccaac
     1681 cagttcatgg tcatcgaatt tgaccgcaat gaaaaacgca ttgtagtctc tcatacccgc
     1741 atctgggaac aaggcaaggt tgaggaagta gagtccgcca agaaagaggc cagggctgaa
     1801 gctgacaaaa ccaaaaaagc cgtgaagacc atccagagta aggtagagaa agccaccctg
     1861 ggcgacctgg gcgtgctggc tgacctgaaa aagaaaatgg aagggaatac tgaagaaggt
     1921 gccgctgaag ctaaacctga gtaagtaaaa atcctactga tacgactgaa ggctgtcttt
     1981 ttttaaaaga cagccttttt ttttaagata aaacactcat ttacgtataa atactatgaa
     2041 tttaatgctg tttaaacttg catataccgc aagtgattat tttctttgtt gtaccatcct
     2101 tttgaatatg ccatcaagtg atttcagtaa gattaccctt ttctgatttg tacttaagca
     2161 tcctttgtcc tggagagcaa gctaccgtac caaacttaag attatgaaca ctcttatgtc
     2221 caggatgtat tttgtccttt tcaacttcat ccgcccttat agcctgttgt ttttcatcct
     2281 gtttgtttcc ctgcaagtaa gagggagcgg acattcagct gcacatattc attcatacac
     2341 atctcagcaa ttatataaag tggacaccgg tggcgggata ctgccgctgc ataatttcac
     2401 actaacggtg acccttaaag aaaataccgt tttcctgaag tgggtggccg aaaatgaaat
     2461 gaatacagag aagtttgtca tccaacggag cacggatggg aattccttta aaaacatggg
     2521 ggaacttcct cccacaggtc cgatcaatat cctgacggag tataacagtg cagatgatat
     2581 tcagggaata gtcagcaatg tggcatacta ccggatcaaa gcggaagaca accggggtaa
     2641 ttttgcctat tccaatgtgg tgcctgttcg cttaaccaaa gctgacggat ttagtttctg
     2701 gcccaatcca ttcagtacgt cactgaatct ttcctataat gccagccttt ctacttctat
     2761 ccgggttgaa atgtatgata atagcggtcg ccgggtcatc cagcaattgt tcaatgtaaa
     2821 cagggggatg aaccagttat cagtaaacgg ggccgcgggt ctgtcgcatg gattatacca
     2881 tgtacgtatc attgacttgc tgaccaatga ggtttctttt tctaaaatgg cgaaataagc
     2941 ctccttctta tttgttgtct attttttcat caggtttctg cacattttct taatttcagc
     3001 cgtgaaaggt ttaccaaaac ttcgacatgg ctgctatatt gaaccgtctg aaatatttta
     3061 acccggtccg gaaactggtg tacatgattg tgggagctgc ttcctatccg gggctggcga
     3121 tcattaacaa actgagcatt tccggtacgg agcatctgaa aggcctccca cgcgaaaaag
     3181 tattattcgt cagtaaccac caaacttatt ttgccgatgt cattactttc ctgcagatat
     3241 tctgtgcagt gaaatggggt aaaatgaaca ggctgggtgt gccctattac ctgttgaatc
     3301 cttttacccg agtcaattat gtggcagcag aggaaaccat gaagggcagc ctgatcagtc
     3361 gcttgttttt actggctggc ggacttacgg taaagcgaac ctggcgggca gaaggaaagg
     3421 aagtaagaag agggctggat ccttccgata cccggaaaat taccagggcc ctggaaagaa
     3481 actgggtcat cacctttcca cagggaacca ccaaaccttt tgcgccaggc agaaaaggga
     3541 cagcccttat tatcaagcag gtaaaaccca tcgtgatccc ggtcgtcata aacggattct
     3601 ggagggcctt taataagaag gggcttaagt ttaaaaagaa aggttctttg ttatccgtaa
     3661 cctttaaagc cccgctcgaa attgattatg aagcacctgc cgacgtgatc ctcgcacaac
     3721 tgatggatgc gatcgaacag agtaagaagt ttatgatgat ggggaaacat cactggcaat
     3781 ccacggataa gtaatcagtc tttcataaag aacgctttta attccaacgc gtcatccggt
     3841 ttcattttgc cacccagtat caaccgcagc tgtcttcttc gcaatgcacc ctcaaataac
     3901 tttttctctt cctcattttc tgcaattacc gcgggaaccg gtcttggttt acggttagga
     3961 tcaagggcta caaacgtata ataggcttca ttgcttttgt atcggaattg ctgcgtcatg
     4021 tcttcacccc atactttcat gtggatctcc atggaagtat taaacgcgcg ggttaccttg
     4081 gcttcgatat gcactgcatt gcccagttta atcgggtttt caaaagagat attatcaacc
     4141 gaggcagtga ccaccggggc attgcaatgt ttctgcgaag caagtgctgc cgcaatatcc
     4201 atccagtaca tcagccggcc acccatgagg ttgccaaata cattggtgtc attggggagt
     4261 acgatttcag tcatgatcgt caggctggcc gacgcttttc ttgcatccat atcttctttt
     4321 ttgcaaatat aggcagttaa atatcagtca tccgtttgtc tgtcatcccg ggacaggttc
     4381 tatttgttgc ggcaacgctt tctgcagtat tttacctgct cccattttaa taagataatt
     4441 gctgccgctg atgtaaggct tggtggtcat caacccgccg tcagcaaact gggtcatgcc
     4501 gtatacatta ggtaccatca cccagttata gctgtcggaa aacatttcca ttggtgcggg
     4561 aaaattagtg tgagctgtaa catcaccggg gagaacaaat ggcatttgta tttgttgacg
     4621 tgtaaacagt ttatatggaa tccctgaaag gaaaatcggt attgat
//
LOCUS       DAOPMW010000261         4654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21544_length_4654_cov_9.466188, whole genome shotgun sequence.
ACCESSION   DAOPMW010000261 DAOPMW010000000
VERSION     DAOPMW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21544_length_4654_cov_9.466188"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLU11_11455"
     CDS             <1..378
                     /locus_tag="PLU11_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59786.1"
                     /translation="NRIFDSICAGADGYLLKKTSPLQLVSALQEVMSGGVAMSPFVAQ
                     KVFQFFRQSNPATPQYNLTPRETVILELLVKGNSYKMIADKSSVTIDTVKKHLQNIYH
                     KLHVSCGTEAVAKALQHKIVKLE"
     gene            631..1227
                     /locus_tag="PLU11_11460"
     CDS             631..1227
                     /locus_tag="PLU11_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59787.1"
                     /translation="MRLSIFMLFALALLSGGLLRAQPFTLDNITPTELLLVDYKKEDT
                     LRRGLINVTTVNQVKDTAWYFVKGLSMFSPVYFGLTAPEGNGNIRVQLCKDNWKTANR
                     SGETGEKRHYQEQFKTEGDFGIMVIAEQKPASYTMITWTGKEAKEVGITSPFKGEGEG
                     AIKEQGGNFLKENYLYILIGLMVVVIAYLVFKMKKSKN"
     gene            1232..1915
                     /locus_tag="PLU11_11465"
     CDS             1232..1915
                     /locus_tag="PLU11_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59788.1"
                     /translation="MKKICLLLFCMVSLQCLAQNEGDNDVVQQMGEDELNQLEESHQN
                     TTNVANGINDAIGHLNTALGLYEDAQDLYNSSQALSSGECSPDFSTTPNSMMSSSCKR
                     EDECAQCYTRAVGELNFIRRQLGRLGCIYQNTKNFTNSAIAFGDNASGIHAVTGLAWQ
                     NARGEIVATMNHFKQTYDNKYRDMIGALERALREIDKCENKYGQEGWFQRSGFIYLEF
                     LKDKYRRND"
     gene            1945..2523
                     /locus_tag="PLU11_11470"
     CDS             1945..2523
                     /locus_tag="PLU11_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59789.1"
                     /translation="MKKYLLLLGCVFLVANVFAQQPPFNQKDLNTSGKKDIRPEKLKP
                     IEPVVAKVAFSALLIGPRELILGVDGNILRRFTRMEFNEGNGFDAVSGVFTAPSAGFY
                     CLVLNADMPNYGCVQDPVPLGMYVMKNNLRHQLFHLPVGYGSGSSSAGFVTFIQLNKS
                     DKVSLSPLPLGCSPGGQQPVVSQVIFSGYKIM"
     gene            2529..3086
                     /locus_tag="PLU11_11475"
     CDS             2529..3086
                     /locus_tag="PLU11_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59790.1"
                     /translation="MLSFILSFVLLFAAPVNADELITEQAVPAGSRPVFSAEMKERRT
                     TDNSRRGGELLQGYTRVEKNEGQVFDPATGIFTVPADGVYHLTGRFTIGCYQCRTREF
                     LDLPVSFDVTVMKNNSPTYHRFMFPVDYTGMESINPNKRAHEFNVLLQLKAGDQIRLR
                     YQSKKCSPEKDAYLYEATFSGMQIN"
     gene            3095..3601
                     /locus_tag="PLU11_11480"
     CDS             3095..3601
                     /locus_tag="PLU11_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPN59791.1"
                     /translation="MKTMKYILLFVYLATGLMLNAQQPVSPGNLPKGPGSAKLKPAAP
                     PDLKVEELSLVSVTRDEANKSINVQVLIRIKNTGAIRCGVSKADAFIKCPADGGRERK
                     LAVSLPVEALNPGASFVKVYTFREAESSFRSADFSFWIKADAANQVNESNETNNTSNR
                     ITGVVPPR"
     gene            3621..4475
                     /locus_tag="PLU11_11485"
     CDS             3621..4475
                     /locus_tag="PLU11_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59792.1"
                     /translation="MKQLFIFCLLLIFFSCNNSAEEKPSVVKDSITKAEAIPPPATVE
                     VPGDSAEPVKEPVTADVGNVSCTRLIFFQPGAEIESVSYDGKGKENSRQVTRILSVSE
                     KGGFTVAKVASTDTEAGEKGKQSKVFYDYKCDGKNIYFDIASMFRTEKKNSDSSFEST
                     PIPFPISVKAGQVLPDAKGVMSAQRGDRKMTMTYTFKKRKVEAMEEITTPAGTWKCYK
                     ISNAVEVEMDIPGMEEKTKAMMKQMQGIMKMTSSTWLSPEFGIVRMETYLNGEMKSRN
                     EVVSFKKK"
BASE COUNT         1322 a          983 c         1170 g         1179 t
ORIGIN      
        1 aaccgcatct tcgattccat ctgtgccggg gccgatggtt acctgctgaa gaaaacttct
       61 cctttacaac tggttagcgc cctgcaggaa gtgatgagcg ggggcgtggc catgagcccc
      121 tttgtggcac aaaaagtctt ccagtttttc cgacagtcca atcccgccac tccccaatac
      181 aacctcactc cccgggagac cgtcatcctt gaactcctcg tcaaaggcaa ttcctacaaa
      241 atgatcgccg ataaaagcag cgtgaccatt gatactgtaa agaagcacct gcagaatatc
      301 tatcacaaac tccatgtaag ttgtggaacg gaagcggtgg cgaaggcgtt acaacacaag
      361 atcgtgaagc tggaataatg agtaatcagt aatgagtaat gagtagtgag tagtgagtgg
      421 tgagtggtga gtggtgagtg tatgtttact tataaatatt agctacgact ttaaaatcct
      481 cttttcctcc ttttctcttt tgaactctta gccgccccgc agggcctccc ttacccgatt
      541 gtgtaattga ctcgcacctt ttcttttcaa atctttacag cgcattcgaa accggcttca
      601 tgccatcgtt ttaccattaa ccagatgtat atgcgcttat caattttcat gctttttgca
      661 cttgcccttt tgtccggcgg actgctgcgg gcccaacctt ttaccctgga taatatcacc
      721 cccacggagc tgctcctggt ggattacaaa aaggaggata ccctgcgccg ggggttgatc
      781 aatgtaacta cggtgaacca ggtaaaggac acggcctggt attttgtcaa gggcctgagt
      841 atgttttcgc cggtttattt cggactgacc gccccggaag ggaatgggaa tatccgggtt
      901 caactttgca aagacaactg gaaaaccgct aaccggagcg gggaaacggg ggagaagcgt
      961 cattaccagg agcagtttaa aacggaaggt gacttcggaa ttatggtaat cgcggaacag
     1021 aagccggcga gttataccat gattacctgg accgggaaag aagcaaagga agtaggcatc
     1081 acttcgccgt ttaaagggga aggagagggt gctataaaag agcagggcgg aaattttcta
     1141 aaagagaatt acctgtatat cctgatcggc ttaatggtag tcgtgattgc ctacctggta
     1201 tttaaaatga aaaaaagtaa aaattaagga tatgaaaaaa atctgtttac tactgttttg
     1261 catggtcagt ctgcaatgcc tggcacaaaa tgaaggggac aatgatgtgg tgcagcagat
     1321 gggggaggat gaacttaatc aattggagga atcccatcag aataccacga atgtcgccaa
     1381 cggaatcaat gatgccatcg ggcatttaaa taccgcgttg ggcttgtatg aggatgcaca
     1441 ggatctgtat aattcgagcc aggccctgag cagcggggaa tgttcgcctg atttttccac
     1501 cacgcccaat tccatgatgt cttccagttg taaaagggag gatgaatgtg cccagtgtta
     1561 tacccgggcc gtaggcgaat taaattttat ccgccgccag ctgggccgtt tgggttgtat
     1621 ttaccagaac actaagaatt tcaccaacag tgccattgcg tttggcgata atgccagtgg
     1681 tattcatgcc gtcaccggac tggcctggca aaatgcccgc ggggaaattg tagccaccat
     1741 gaatcatttc aaacaaacct atgataacaa gtaccgcgat atgatcggtg ccctggaaag
     1801 agccctgcgg gaaatagata agtgtgaaaa caaatacgga caggaaggtt ggtttcaacg
     1861 gtccggattt atttacctgg agtttctgaa agataaatac cggagaaatg attgatcatc
     1921 ggacagaaat caaaaataca ttatatgaaa aaatacctgt tgttactggg ttgtgtattt
     1981 ctggttgcaa atgtctttgc ccagcaacca cccttcaatc aaaaagacct gaatacaagc
     2041 ggtaaaaagg atatccggcc tgaaaaacta aagccgatag aaccggtagt ggcaaaagtg
     2101 gcttttagtg cattactcat cggtccgcgt gaattgatat tgggggtgga tggtaatatt
     2161 ctccgccggt ttacccgaat ggagtttaac gaaggaaacg gatttgatgc ggtttctggt
     2221 gtattcactg cgccatctgc cggattttat tgtctggttt taaatgcaga tatgcccaat
     2281 tatggctgtg tacaggatcc ggtgccgttg ggcatgtatg tgatgaaaaa taatctgcgg
     2341 catcagttgt ttcatttgcc ggtgggatat ggcagcggaa gcagttcggc cgggtttgtt
     2401 acgttcattc agttaaataa gagtgataaa gtttctcttt caccattacc gttaggctgt
     2461 tcaccaggcg ggcaacaacc tgtagtcagt caggttattt tcagtggtta taaaataatg
     2521 taatatctat gctatccttc attctttcct ttgtcttatt attcgctgcc ccggttaacg
     2581 cagacgaatt aataaccgaa caagcggttc ctgccggaag cagaccagtc ttttctgcgg
     2641 aaatgaaaga aagacgaacg acggataata gcagaagggg aggagaactg ctgcaagggt
     2701 atacccgggt ggaaaagaat gaaggacagg tttttgatcc ggctaccggc atatttaccg
     2761 ttcctgctga tggagtttat catctcaccg gtcgttttac cattgggtgt taccaatgcc
     2821 gtacccggga attcctggat ttgcccgtct cctttgatgt tacggtgatg aagaataatt
     2881 cacccactta tcaccggttc atgtttccgg tggattatac cgggatggaa agtattaatc
     2941 ccaataaacg ggcgcatgaa tttaatgtgc tgcttcagtt aaaagccggt gatcagatcc
     3001 ggttacgata ccagtctaaa aagtgcagtc cggaaaaaga cgcctatcta tatgaggcaa
     3061 cgtttagcgg catgcagatt aattaattaa accaatgaaa acgatgaaat atatactgtt
     3121 attcgtttac ctggccaccg gcctgatgct gaatgcccaa cagcctgtta gtcccgggaa
     3181 cctgccaaaa ggaccgggat cggcaaaatt aaaaccggct gctccgcctg atctgaaggt
     3241 ggaggaactc agcctggtat cggtaacccg tgatgaagcc aacaaatcca tcaacgtgca
     3301 ggtactgatc cggattaaga ataccggggc gatccgttgc ggggtttcaa aggcggatgc
     3361 ttttataaaa tgtccggcag atggaggtag ggaacggaaa ttggctgttt ctttgccggt
     3421 ggaagccttg aatcccggtg cctcttttgt gaaagtgtat accttcaggg aagcggagag
     3481 cagttttcgc tccgctgatt tcagtttttg gataaaggcg gatgcagcga accaggtgaa
     3541 tgagtcaaac gaaaccaata atacgtcaaa ccggataaca ggagttgttc ctccaaggtg
     3601 atctggaaaa tatatcacac atgaagcagt tgttcatttt ttgtttacta ctgatatttt
     3661 tttcctgcaa taattcggcg gaagaaaagc cctccgttgt taaagattct attacaaagg
     3721 ctgaagcaat tccgccgcct gcaaccgttg aagtaccagg tgattcagcg gagccggtaa
     3781 aggaaccggt aacagccgat gttggtaatg tatcctgtac ccgcctgatc ttttttcaac
     3841 cgggagcgga aatagaatcg gtaagttatg atgggaaagg aaaagaaaat tcacgccagg
     3901 tgacccgaat attatccgtg agcgaaaaag gaggctttac cgtggcgaaa gtcgccagta
     3961 ccgatacaga agccggggag aaaggcaagc agtcgaaggt tttttatgac tacaagtgcg
     4021 acgggaagaa tatttatttt gatattgcct ccatgttccg gacggagaaa aagaacagcg
     4081 attccagttt tgaatccacc ccgattccat tcccgatcag cgtaaaggcc gggcaggtat
     4141 tgccggatgc taaaggggtt atgagcgctc agcgcggcga ccggaaaatg acgatgacct
     4201 atacctttaa aaaacgaaag gtggaagcga tggaagaaat caccacaccg gccggtacct
     4261 ggaaatgtta taagatcagc aatgccgtgg aagtggaaat ggatataccc ggtatggaag
     4321 agaaaaccaa agccatgatg aaacaaatgc agggaataat gaaaatgacc agcagtacct
     4381 ggttatcgcc cgagttcggg attgtgagaa tggaaacata tctcaatggg gaaatgaagt
     4441 caaggaatga agtcgtgtct tttaaaaaga agtagtggtc ctgcccaact aaatttagtt
     4501 atgaaatatt tcttcttcct cctgttgttc tccgcctgtt tatccgcccc tgcccaaccc
     4561 ggaaaatttg ccggcactaa aaaatcgctg atcggtaaag aatatacaga cagccgcctg
     4621 atgcccggat tgaaaagctg gaccttcaga gaag
//
LOCUS       DAOPMW010000262         4600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21917_length_4600_cov_9.212321, whole genome shotgun sequence.
ACCESSION   DAOPMW010000262 DAOPMW010000000
VERSION     DAOPMW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21917_length_4600_cov_9.212321"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(451..1254)
                     /locus_tag="PLU11_11490"
     CDS             complement(451..1254)
                     /locus_tag="PLU11_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPN59793.1"
                     /translation="MKKLLLFGMALSLAISNLTAQQNQGKVTYNRTVKLQMSFAGMPG
                     GLEQQLPSSRTDKYELVFGNNQSLWKQGEQEPEEEQSFGDGGMQIRMVVAGSNDVLYC
                     NFDNSRRVEKREMFDKTFIVDDTITKLKWKMTGESKTILNHNCMKAEAVRISKRMTMN
                     MDNGKMERKEIDDTAKIVAWFAMDIPVSAGPSEFQGQLPGLILELDVRNGDQVYLANS
                     IAETADLAVIKEPTGKKKYTQEEFKKEREKMMEEMNQNNGGNRRVIRMN"
     gene            complement(1345..2142)
                     /locus_tag="PLU11_11495"
     CDS             complement(1345..2142)
                     /locus_tag="PLU11_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPN59794.1"
                     /translation="MKKIFLAPLLLILFLGFAAAQQNQGRVVYNLTIRLQFSFGGQST
                     GLEQLLPASRTVKYELLFANNQSTWKQKPETEETLPDFGSEGNTMKIVVAGSEDLLYC
                     NFDTRMKTEKRMLYDKQFLIEDSLSKLTWKLTGETKKILDHVCVKAESIRIKKEYITT
                     VEGGTFGKKEVDDTSKIIAWFTTEIPVSAGPSEFQDQLPGLILELNINEGEQLFSAVS
                     IDPLADLSEIKAPAGKKKYTQKQFLKEKEKLADEINSRGGNKRIFRM"
     gene            2289..3743
                     /locus_tag="PLU11_11500"
     CDS             2289..3743
                     /locus_tag="PLU11_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN59795.1"
                     /translation="MLISIMGIAAFLFYWLTENYKREEKTLRLKTDMVFREEMMKLQV
                     ARLKLGKSGTDSTDKGRVRVIMGDRNRGPHRPEEMEEEVVSSINIIRDKLADSMVTFS
                     PTGKKPVMITMQTSTSFDTGNDSMNIEMNVPPVYAPGNPIFSFLYGVDSLQDSIRVTE
                     IDSVIRFALQKEKIGIPFTIIKSAKSVSRGNRNFGSVTIGLKNPQTYSLAIGNSWPFL
                     VKRIAMPVIFSLLLLGITIAAFLVLYKNLRKQRKLAELKNEFISNITHELKTPIATVG
                     VAIEALKNFNAIDNPERTREYLDISQNELHRLSLLVDKVLKLSMFENQGIELKYETLN
                     LRGIVTEVVESLKLQLEKFKATVDVQYNGETELRGDRLHLLSVVFNLLDNALKYRSTD
                     KEVRIEVQINGTADAVQLIIRDNGIGISPEYQEKIFEKFFRVPHGDTHNAKGYGLGLS
                     YVAQVIKKHEGTITVQSVPGESTAFTITLPKSQS"
     gene            3740..4429
                     /locus_tag="PLU11_11505"
     CDS             3740..4429
                     /locus_tag="PLU11_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59796.1"
                     /translation="MNKTKVLYVEDELFLGKIVRESLESRGFEVLMEEDGAKVLDRFK
                     KGKPDICVLDVMLPNKDGFTIADEIRELDEEVPIIFLTAKTQTEDVVKGFTLGGNDYI
                     RKPFSMEELIVRMQHLLRQKTEGPQKIQGNTVTMGKFSFQLNRQLLVIGTEEKKLSFR
                     ESELLKLLYENRDKIIDRRDILNLLWGNDSFFNSRNLDVYITKLRGFLKPDPSLEIIT
                     IKGVGYRFVVG"
BASE COUNT         1211 a         1029 c         1040 g         1320 t
ORIGIN      
        1 cacaccggcg gtcatcagca acagatccct gagcagccgt ctgaatgtaa tcgtcagttc
       61 ccgataggat ttcgcataac ggtagcgaga tattttgttt tcgccatttg ctgctgctgc
      121 ctgcggaacc cgggtgctta cgtcaataag ttttcttaag aattgcttca ttcggtgggg
      181 tttcgttggt tctttcaaat ttaccccttt tcgccgggaa cagcccattc taagaagcaa
      241 atcttcaaag aatgtcagcg tactttgtac ttctaacttc gtactttctt aaatacaagg
      301 ttcctcactt cgttcggaat gacgggctga ttagggggga gagaaaaaat acaagagttc
      361 tcgctacgct cgaaataaca gtcaatagtg cataaaaaaa aggttgctcc gttttggtgg
      421 caacctcttt ttttattgtt tgatacgtgg ttaattcatc cggatcaccc gtcggttgcc
      481 accgttattc tgattcattt cttccatcat cttttcccgt tctttcttga actcttcctg
      541 tgtatacttt ttcttgccgg tgggttcttt gattacagct agatcggctg tttcggcaat
      601 actgtttgcc aggtatacct gatcgccatt gcgcacatcc agttcgagga tcagtccggg
      661 cagttgtccc tggaattcag aaggaccagc ggatacggga atgtccatgg caaaccaggc
      721 tacgattttc gccgtatcgt cgatttcctt tctttccatt ttgccattgt ccatattcat
      781 agtcatacgt ttgctgatcc gcacggcttc cgctttcatg caattatggt tcaggatggt
      841 ttttgattca ccggtcattt tccacttcag tttggtaatg gtatcatcca caataaaggt
      901 cttgtcaaac atttcccttt tttccacgcg gcggctgttg tcaaaattgc aatacagtac
      961 atcgttgctg ccggctacca ccatccggat ctgcatgcct ccgtctccaa aggactgctc
     1021 ctcttcgggt tcctgttcgc cttgtttcca gagcgactga ttattcccaa ataccagttc
     1081 atatttatcg gtccggctgc tgggcagttg ctgttccagc ccaccgggca taccggcaaa
     1141 actcatctgg agtttaacgg tccggttata agtgactttt ccctggtttt gctgggcggt
     1201 aaggttggag atggccagtg agagcgccat tccgaataaa agcagttttt tcatgttttt
     1261 ctatttgtcc caaagctaca ttcttgcgtg ttaaccagtt tgaaacaaag gttaataaag
     1321 gttaatacgc cagatcgtat caggttacat cctgaaaatt cgtttatttc ccccacggct
     1381 atttatttca tccgctaatt tttccttttc tttcaaaaat tgcttctggg tatatttctt
     1441 ctttccggca ggtgctttta tctcagacag gtctgcaagc ggatcaatcg aaacggctga
     1501 aaaaagctgt tcgccttcat ttatattcag ttcaaggatc aatcccggta attgatcctg
     1561 aaactccgaa ggtccggcgg atacaggaat ttcagtagtg aaccacgcga taatcttaga
     1621 cgtatcgtcc acttcctttt taccaaaggt tcctccttca acggtagtaa tgtattcctt
     1681 ttttatccgg atcgattcag ctttgacaca aacatgatca aggatctttt ttgtttcacc
     1741 ggtcagtttc caggttaatt tggagagact gtcctctatc aggaattgtt tatcatataa
     1801 cattcttttt tccgttttca tccgggtatc gaagttgcag tataacagat cctcgctacc
     1861 agccaccaca attttcatcg tatttccctc tgatccaaaa tcagggagtg tttcctccgt
     1921 ctccggcttt tgtttccagg tggactgatt attagcgaat aataactcat acttcacggt
     1981 acggctggca ggcaggagct gttcaagtcc tgtggattgg ccaccgaaac tgaattgtaa
     2041 ccgtatggtc aggttatata ccactcttcc ctggttttgc tgcgcagccg cgaagcctaa
     2101 aaaaagaatc agtaaaagtg gggctagaaa tatttttttc atggttttag tgtttcccaa
     2161 atctagatta cagcaggtta atccatcttg taataaggtt aataaaggtt aaggatcgat
     2221 tacatgcccc cggttttagc tatttttgat gtgcaatgaa aagtcgccgc atacctggcc
     2281 ctctcctgat gctgatttcc ataatgggaa tcgcggcctt tcttttttac tggcttacgg
     2341 aaaactataa acgggaagaa aaaaccctgc gactgaaaac agacatggtc ttccgcgaag
     2401 aaatgatgaa attgcaggtc gcccgtttaa aactgggtaa atccggtacg gattcaacgg
     2461 ataagggacg ggttcgtgta attatgggtg accgcaacag gggacctcac agacccgaag
     2521 agatggagga agaagtggtt tcttccatca atattatccg ggataaactg gcggattcaa
     2581 tggtgacatt ttccccgaca ggaaaaaagc cggtcatgat taccatgcag acttcaacat
     2641 ctttcgatac gggcaatgat tccatgaata ttgaaatgaa cgttccgcct gtctatgctc
     2701 ccggcaatcc gatatttagc tttctctacg gcgtggattc cctgcaggat agtatccggg
     2761 taactgaaat tgattcggtg attcgtttcg cccttcaaaa ggaaaaaatt ggaattccgt
     2821 ttaccattat caagtcagcc aaatccgtat caaggggaaa ccgaaatttc ggtagcgtta
     2881 cgatcgggct caaaaatcca cagacctatt cgctggccat tggtaacagc tggcccttcc
     2941 tggtgaagcg cattgccatg ccggttattt tttccctgtt actgctgggc atcacgatcg
     3001 ccgctttcct tgtcttgtat aaaaatctcc ggaagcaacg caagctggcc gaactcaaga
     3061 atgagtttat cagcaatatc acccatgaat taaaaacccc gattgccacg gtaggcgtgg
     3121 ccattgaagc gctgaaaaat ttcaacgcca ttgataatcc ggagcgcacc cgggaatacc
     3181 tcgatatttc gcagaatgaa ttgcaccggc ttagtctgtt ggtggataaa gtactcaagc
     3241 tgtcgatgtt tgaaaaccag ggaatcgaac tgaagtacga aacattgaat ctgcgtggta
     3301 tcgtcaccga agtggtggaa tcgctgaaac tgcaactgga gaaattcaag gcaacggttg
     3361 atgtacaata taacggagaa acggaattgc ggggtgatcg ccttcacctg ttgagtgtgg
     3421 tgtttaacct gcttgataat gccctgaagt accgaagcac tgataaagag gtaaggattg
     3481 aagtccagat caacggaacg gccgatgcgg tacaactgat tatccgcgac aatggaattg
     3541 gtatttcacc ggaataccag gaaaagatat ttgaaaaatt cttccgggtg ccgcatggcg
     3601 atacccataa tgctaaaggc tacggacttg gattaagtta tgtggcccag gttatcaaaa
     3661 aacacgaagg aactattacc gttcagtcag tacccggtga aagcaccgca tttaccatca
     3721 ctttaccaaa gagccagtca tgaacaagac caaagtatta tatgtagagg atgaactgtt
     3781 tctgggaaag atcgtccggg agagcctgga aagccgcggc tttgaggtgt tgatggaaga
     3841 ggatggcgcc aaagtgctgg accggtttaa aaaaggtaaa ccggatatct gtgtgctgga
     3901 tgtaatgctg cccaataagg acggttttac cattgccgat gagatccgcg agctggatga
     3961 ggaagtgccg atcatttttc tcaccgccaa aacccagacc gaggatgtcg tgaaaggatt
     4021 tactcttggc gggaatgatt atatccgcaa gcccttcagt atggaagaac tgatcgtgcg
     4081 gatgcagcac ctcctgcggc agaaaacgga aggaccgcag aagatccagg gcaatacggt
     4141 gacgatggga aaattcagtt tccagctcaa ccggcagctg ctcgtaatcg gtaccgagga
     4201 aaagaaactc tccttccgcg aaagcgaatt attgaaatta ttatacgaaa accgcgataa
     4261 gatcattgac cgccgcgaca tccttaatct tctttggggc aatgattcct tcttcaacag
     4321 ccgcaacctg gatgtctata tcactaaact gcggggtttt ctgaaaccgg atccttcttt
     4381 agaaattatt acgataaaag gggtggggta tcggtttgtt gtaggataat aaagaaccaa
     4441 gaagccaaga cgccaagatc caaagaaaaa tcaaatgaca aatttcaaaa aagcatcaaa
     4501 aggaaataat caaagccttc tgtgaaagtt ctcaggagga ttggtgcttt tttggagttt
     4561 ctcttttggt ccttctttga tccttgaatt ttggttcttc
//
LOCUS       DAOPMW010000263         4596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21951_length_4596_cov_5.677384, whole genome shotgun sequence.
ACCESSION   DAOPMW010000263 DAOPMW010000000
VERSION     DAOPMW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21951_length_4596_cov_5.677384"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLU11_11510"
     CDS             complement(<1..233)
                     /locus_tag="PLU11_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59797.1"
                     /translation="MGGKEEAVQIKWGIGSGNANGSINYQIVDYLREMFNIQFSMLNK
                     QQARLLTANCQLPTANCQLPTANCRLPTANCRLP"
     gene            268..2448
                     /locus_tag="PLU11_11515"
     CDS             268..2448
                     /locus_tag="PLU11_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN59798.1"
                     /translation="MRKLFLLLLLAPVWVMAQTKPITLEDIYKKGTFRGEFVPAVFDT
                     TQKEPELKLDEVKDETGKSFGRADDQVENPVNKNFWLLKKGSEQIYRRSSKSFVYLYD
                     MVTGKATKLENEKVLHPTFSPDGSKVAYVKENNLYVYDIAAGKSTAVTTDGKWNFVIN
                     GNCDWVYEEEFEFTRAYEWSPKGNFLAYYRFDESQVKEYNMTMFDNAHNKDYRYKYPK
                     AGDGNSSVDIYIYDVAKGSKAKAQYEQGDIYIPRIKWTRDDNTLVLYWMNRYQNDLKL
                     LATNASTGASSLLYQETNKYYVEINDDWWYLKDGKNFLFTSEMNGYKHLYLYSLDGKT
                     KTQVTKGNYEVTDINGVDEANKRIYFTMAYPRPMDRNLFVTDFAGKKTYALTQGEAWH
                     RVEFNEDFTKFFAFRSDLNTPQTVTLNKLVVDKKKGISAVVEKTMNESAKQKAKLAEY
                     GYGKADFIRVPNSKGDTLNGWMLKPAGFDPAKKYPVLFCNYGGPGSQQVANRFGAVSA
                     WHQLLAQKGFIVVSVDNTGTGFRGEEFKKKTYLQLGKFEIEDQIDAAKYLGTMPFVDK
                     DNIGHWGWSYGGFMSSLAITKGNEVFSAAVAVAPVTNWRYYDNIYTERYMRTPQENAK
                     GYDDNSPINFTDKIKGKYLIIHGTADDNVHFQNATQMITALVKSNIDFESAYYPNKNH
                     GIGGMADNTTFHLWSKMTNWILENMGNDNTQKPAGGQVKQPKGF"
     gene            2907..3464
                     /locus_tag="PLU11_11520"
     CDS             2907..3464
                     /locus_tag="PLU11_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPN59799.1"
                     /translation="MRKILVAVSGASGSVYASLLMNRLLAIKDQWTEVSVIMTTNAKE
                     VWKTELSNETWADYPFRYFSTTDFSAPFASGSGQYDTMIIIPCSMGTLGRIATGISND
                     LITRAADVVLKERRKLICVIRDTPYNLIHIRNMETVTLAGGVICPATPSFYSRPQTIE
                     EAAATVVDRVLDLAGLRIDTYRWGK"
     gene            complement(3519..>4596)
                     /locus_tag="PLU11_11525"
     CDS             complement(3519..>4596)
                     /locus_tag="PLU11_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59800.1"
                     /translation="SGLAAGSYTITAKNAAGCTGTATFTVSNPPNPCTGITITVTATS
                     INPTTVGGTDGSISATAAGSSNYTFSINGGAFQASGNFTGLPAGTYSIVAKDANGCQG
                     SASFTLVNPNPCAGVTISVTGTTTNPTSSSASDGSISATASGSTGFTYNLNGGAYQAS
                     GNFTGLGGGSYTVGAKDGNGCTGTANFTLTAPNPCAGVTITVSNTTVGNIPCQTNNGT
                     LTATAAGGTGPYLYSLNSGAFQASNIFLNLGPGTYAVQAKDANSCNGASGNTTLANRP
                     AGPLFSDVKAVLQANCVSCHNNTQQEGGMNWMVDCNIVANKDRIKARAVDASPSAMPP
                     TGLLPATERQKITSWINAGGKFTD"
BASE COUNT         1280 a         1054 c         1084 g         1178 t
ORIGIN      
        1 ggcagtcggc agttggcagt cggcagtcgg cagttggcag tcggcagttg gcagttggca
       61 gttggcagtt ggcagttggc agtcagcagt cgggcttgtt gtttattgag cattgaaaat
      121 tgaatattga acatttctct caaataatca acaatctgat aattaatact cccattggca
      181 ttcccgcttc ctatccccca ttttatctga acggcttcct cctttccccc caatccccta
      241 catttacgta ccaaaaatta tcacactatg cgtaagcttt ttttgttgct gttactggca
      301 ccggtatggg tcatggcgca gacaaaaccc atcactttag aagatattta taagaaaggt
      361 actttccggg gagaattcgt tcccgcggta tttgacacca cgcagaaaga accggaactg
      421 aaactggatg aagtgaaaga tgaaaccggc aaatcttttg gcagggccga cgatcaggtg
      481 gagaaccctg tcaataagaa tttctggtta ctgaaaaaag ggtcggaaca gatttaccgc
      541 cgttcatcga aatcctttgt atatctctac gatatggtta ccggcaaagc cactaaactg
      601 gagaacgaga aagtattaca ccctaccttt tcccccgatg gcagtaaagt agcctatgta
      661 aaagagaata atttatacgt gtatgatatt gctgccggta aatccactgc ggtaaccacc
      721 gatggcaaat ggaattttgt cattaacggt aactgtgact gggtatacga agaagaattc
      781 gaatttaccc gggcatatga atggagtcca aaaggaaatt tcctggccta ttaccggttt
      841 gatgaaagcc aggtaaagga atacaatatg accatgttcg acaatgctca taataaggac
      901 taccggtaca aataccccaa ggctggtgat ggaaattcct cagtggacat atacatttat
      961 gacgtggcca aaggcagcaa ggcaaaagcg caatacgaac aaggcgatat ttatatcccc
     1021 cgcatcaaat ggacccggga tgacaatacc ctggtgctgt actggatgaa ccgttatcag
     1081 aatgacctga aactgcttgc caccaatgcc agcaccggcg ccagcagcct gctgtaccag
     1141 gaaaccaata aatactatgt cgaaataaat gatgactggt ggtacctgaa agatggaaaa
     1201 aatttccttt ttaccagtga aatgaatggc tataagcacc tttacctgta cagcctggat
     1261 ggcaaaacaa aaacccaggt caccaagggc aattatgaag taactgatat caatggcgtg
     1321 gacgaagcaa ataagcgcat ttactttacg atggcttatc cccgcccgat ggaccgcaat
     1381 ttgtttgtca cggatttcgc tggcaaaaaa acgtatgcac ttacccaggg agaagcctgg
     1441 caccgggtgg aattcaatga agatttcacc aagttctttg cttttcgttc agaccttaat
     1501 accccgcaga cggtgaccct gaataaattg gtggtggata agaaaaaagg gatcagcgcc
     1561 gtggtggaaa agacaatgaa tgaaagcgcc aaacaaaaag caaaacttgc tgaatatgga
     1621 tatggcaaag cggatttcat ccgggtgccc aatagcaagg gcgacaccct gaatggctgg
     1681 atgctgaagc ccgccggatt tgaccctgct aaaaaatacc cggtgctgtt ctgcaactac
     1741 ggggggcctg gttcacaaca ggtagccaac cgatttggtg ccgtaagtgc ctggcaccag
     1801 ttactggctc agaaaggttt cattgtagtg agtgtcgata atacaggaac cggtttccgt
     1861 ggagaagagt ttaaaaagaa aacctacctc cagcttggta aatttgagat tgaagaccag
     1921 atcgatgcgg ccaaatacct gggtaccatg ccctttgttg ataaagacaa tattggtcac
     1981 tggggctgga gctatggtgg ctttatgagt tcgctggcca tcactaaggg caatgaagtg
     2041 ttcagcgcag ccgtagcagt ggcaccggta accaactggc gctattatga taatatctat
     2101 accgaacgtt atatgcgcac cccgcaggag aatgccaaag gatacgatga caactctccc
     2161 atcaacttca ccgataaaat caaagggaag tacctgatta ttcatggaac ggcggatgac
     2221 aatgtgcatt tccagaatgc gacacaaatg atcaccgcat tagtaaaaag taatattgat
     2281 tttgaaagtg cctactaccc gaacaagaat cacgggatcg gcggcatggc ggataatacc
     2341 acattccatc tctggagcaa gatgaccaac tggatcctgg agaatatggg aaatgataat
     2401 acacagaagc cggcgggtgg tcaggtgaag cagccgaagg gattttgatg gtgagtggag
     2461 aatggagagt ggtaagtggt gagtgggggg cgtaactgat cggatatacc ctaaataaat
     2521 tgagccctcc gaattacgga gggctcaatc attatatgat gtattcccac tttccactct
     2581 ccattttcca ctctccactt acctaaaaaa aagaaccccc gatgtaaaat cggggggccc
     2641 taatcaaaaa ccattaacca ttaaacctta tcctatgaaa attcattgct aatatcggca
     2701 ttaatgcgaa atcacaaaac atttgtgccg tgctttttag ccgaaatgga caaaatcatc
     2761 ctcaaatcac gattttttga ctttactcaa ctcattcata atgagatttg tagacgatgt
     2821 actacatagg tcatgtgcaa tcgtttgcgc aatttaccct accttacggc aactttctcc
     2881 agttttacag ggttatacag ttcaatatga gaaaaattct tgtggccgtt tccggcgcca
     2941 gtggttccgt ctatgcttct ttactgatga accggttact ggctataaaa gatcagtgga
     3001 ccgaagtatc cgtgatcatg accacgaatg ccaaagaagt ctggaaaacg gaactgagta
     3061 atgagacctg ggcggattat cctttccgtt atttcagcac caccgatttc tctgcccctt
     3121 ttgcatctgg ttcgggccag tatgacacga tgattattat tccctgttca atgggtaccc
     3181 tcggacgtat tgccacgggc atttccaatg acctgattac ccgtgcagct gatgtggtgc
     3241 tgaaagaaag aagaaaactg atctgtgtga tccgtgacac gccttataac ctgatccata
     3301 tccgcaatat ggaaaccgta acgctggctg gtggtgttat ttgtccggcc accccttcct
     3361 tttacagtcg tccgcaaact attgaagaag ctgctgccac ggttgtagac cgcgtgcttg
     3421 acctggccgg tctccgtatt gatacttacc gttggggtaa ataaaagggc cataccagtg
     3481 aactggtacg gccccaaaca tacaccatca tgaaccaact aatcggtgaa ctttccacca
     3541 gcgttaatcc agctcgttat tttctgacgt tcggttgccg gtaataatcc ggtcggcggc
     3601 atggcagaag gtgaagcatc cactgctctt gcctttattc tgtccttatt tgctacaata
     3661 ttgcaatcta ccatccagtt cataccacct tcctgttgcg tgttgttatg acaggaaaca
     3721 caattggcct gcaacacggc tttcacgtca ctgaataatg gaccggccgg gcgatttgcc
     3781 agcgtggtgt taccacttgc gccgttacag gaattcgcat cttttgcctg aactgcatat
     3841 gtgcccggac ccaggttaag gaatatattt gatgcctgga aagcaccgct gttgagacta
     3901 tacaggtaag ggcctgttcc tcccgcagca gtcgcggtta atgttccgtt attcgtttga
     3961 caagggatat tcccaaccgt tgtgttactt acagtgatgg ttacccccgc acaaggatta
     4021 ggagcagtca gcgtgaaatt agccgtacct gtacagccgt tgccatcttt agccccaact
     4081 gtatatgaac caccgccaag gcctgtaaaa ttgccactgg cctggtaagc acctccattc
     4141 aggttatacg tgaatccggt actgccggat gctgtagcac tgatactacc atccgaagct
     4201 gaagaagatg tagggtttgt ggtagtacca gttacagaaa tggtcactcc tgcacaagga
     4261 ttgggattta ccagtgtaaa gctggcactg ccctgacaac cattggcgtc tttggcgaca
     4321 attgaatatg ttccggcggg gagtccggtg aaatttccac tggcttgaaa agctccacca
     4381 ttgatgctga atgtatagtt gctgcttcct gctgcagtgg cactgattga tccgtccgta
     4441 ccccctactg ttgttgggtt gatactggtg gccgttactg ttatggttat accggtacag
     4501 ggattaggtg gattagatac agtaaatgta gcagtaccgg tacatccggc cgcgttttta
     4561 gccgtaatgg tataacttcc tgccgcaagg ccgctg
//
LOCUS       DAOPMW010000264         4591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_21982_length_4591_cov_8.861772, whole genome shotgun sequence.
ACCESSION   DAOPMW010000264 DAOPMW010000000
VERSION     DAOPMW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21982_length_4591_cov_8.861772"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLU11_11530"
     CDS             <1..245
                     /locus_tag="PLU11_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59801.1"
                     /translation="LTALQDLDIEGDGFSSMKPVVLSDLKFLESIPWLKRLCLKMVRV
                     KDKDYSPILKIKELKWLSLPHYKEVEQQMHLFKHLN"
     gene            467..1783
                     /locus_tag="PLU11_11535"
     CDS             467..1783
                     /locus_tag="PLU11_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN59802.1"
                     /translation="MRPFTPFKNLLNVDGLPAEAPNSRDNFLVREGNQVHKALIYIEA
                     FYGFFFLFVTLIQGYYYQSVLNAALFVFSCLAYIIQYKGYAFYSKIFNLSQLIIILAL
                     MFYFPASPYGIHANDSVLAFYIPVSVGTLIAFQGKENKYGYIFSGLILLVSLTLIVFD
                     PHYLTDQPVATLTGISYDLLYNIIGAAIATFTEVAYILALNNRLNESLTKANHELDNF
                     VYIVSHDLRSPLLLTKGLLDISKLKVGEKEEVLKYLDLAGKSINNLDDIIKEILAYSR
                     NSRTGLQYESFDLKTIIKEIKDGLEIKDGPSFSFLEEYNGDSRIYSDKGRLHTILRNL
                     ITNAVKYRKKELNDTYVKIVFGKTGNRFTITVSDNGQGIARESMDKVFDMFYRATSAA
                     PGTGLGLYICKEMLEKMGAEFYLDSTEGQGTSFTFTLEQATPVVPA"
     gene            1943..3376
                     /locus_tag="PLU11_11540"
     CDS             1943..3376
                     /locus_tag="PLU11_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN59803.1"
                     /translation="MLSNIHLRNLLDVDIHFSKGYYSTDNILVSKGDRVHTTLLSIVI
                     FFGVVYTVISLIQQYHYQAFLSFSCGVFAFFSWLIHRRGYTLLSKLVNFTQLIAFIAL
                     MFYFPSSPYGEHINDSVLAFYVPVLIGTLIAFQGKQKVYGLVLAVIVIFVISFLILTD
                     IHYPEHPVLQNYTGSNYDMLFNILGAAVATFAEVAYILALNDRLNSSLVKTNHELDNF
                     IYIVSHDLRSPLLSTKGLIDLAKIKIDEREQVLKYLSLAGQSINNLDHIIHEILTYSR
                     NARTELKEEEFELKDVVKEVFDGLRFSAGSGFSFLEEYSGETKIYADKNRLSTVLRNI
                     ISNSVKYKKKDIDNPFVLLRFENNNLHCSFSITDNGEGIPLESQHRVFEMFYRGTSSA
                     TGTGLGLYICKEMLDKMNAVFAISSKPGEGTTFNIQLNKTATSLAKEKKEPAFNTTKQ
                     SGSPTASVKLNEQLNSIQGFSSFNQAG"
     gene            3562..>4591
                     /locus_tag="PLU11_11545"
     CDS             3562..>4591
                     /locus_tag="PLU11_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPN59804.1"
                     /translation="MPRRLIPIFLILTCLCTGAAAQQALPDFTRIDKQARSIRFDGDL
                     IKLTTTLTSPYPDKLSKVRSIFIWITEHIRYDWKFFNKDEEIEIPECKSGQDCDEVLA
                     NWEKKYLSNILRKGKAISDGYARLFTRMCDIAGLEAATINGHTKTKLYQIGNAGPVNH
                     SWNVIWLDTTWHLLDATWSAGYTTEDENTGKLNGFVKQYNDYYFLTPFAEFSRNHFPQ
                     YTRWVLKPGYTKDKFAANPYFDPDILSKIKLHSPTTGMINGKKGDTIHFKFEYTGTIE
                     KLQVNTNLYRNAEVWGWEQINKRKKVWVQDTFALKRQRYTPFVKTGNLYEFDYVIPEN
                     SLYYLDILF"
BASE COUNT         1322 a         1115 c          920 g         1234 t
ORIGIN      
        1 ccctgaccgc cctgcaggat ctcgatattg aaggagatgg gttctcctcc atgaaaccgg
       61 tagtactttc tgacctgaaa ttccttgaat ctattccctg gctgaaacgg ctctgcctga
      121 aaatggtcag agtaaaagac aaagattatt cacccatcct taaaataaag gagttgaagt
      181 ggctgagtct gccgcattat aaagaagtgg aacaacagat gcacctgttc aaacacctta
      241 actaagtacg aagtacgaga tacgaatacc aaagtatccc gacgtactgt cgccataaaa
      301 cgatattcat ccgtctaagg atatccccaa ctcctggcac caacacccgg caactgatct
      361 gatacttagt taaaaataag taccggattg ttttcagatc aacgtttttt aaaccccgaa
      421 ttgatacctt taataccctg gctttttgaa aaacgatcgg gaaacaatgc gaccatttac
      481 tccttttaaa aatctgctga atgtggatgg ccttcctgcc gaagcgccta acagccggga
      541 taatttcctg gtcagggaag gaaaccaggt tcataaagcc ctgatctata ttgaagcctt
      601 ttacggattt ttcttcctgt tcgtgaccct gatacagggc tattattatc aatcagtatt
      661 gaatgcagcg ctttttgttt tttcatgcct ggcttatatt atccaataca aaggatatgc
      721 tttttattcg aaaatcttta acctcagcca gctgatcatt attctcgccc tgatgtttta
      781 ctttccggct tccccttatg gaatacacgc caatgacagt gttttggcct tctatatacc
      841 ggtaagcgta ggcacactga ttgctttcca gggaaaagaa aacaagtatg gttatatttt
      901 ttcaggtctg attttgcttg tttcgctgac cctgattgtt ttcgatccac actacctgac
      961 cgatcaacct gttgccacac tgaccggaat cagttatgac cttctctata atattatcgg
     1021 tgccgctatc gccactttca ccgaagtagc ctacatactg gcgttgaaca accggttgaa
     1081 tgaaagcctc accaaggcta atcatgagct ggataatttt gtatatatcg tgagccacga
     1141 tctgcgttcc ccgttgctgc tgaccaaagg attgctggat atttcaaaac ttaaagtcgg
     1201 ggagaaggaa gaagtcctga agtaccttga tctcgcgggc aaaagcatca ataacctgga
     1261 tgacattatc aaagaaattc tggcctattc ccgcaattca agaacaggac tgcagtatga
     1321 atcgtttgac ctcaaaacca tcataaagga aataaaagat ggattggaaa taaaggacgg
     1381 cccgtccttc tccttcctcg aagaatacaa cggggatagc cggatttatt ccgataaagg
     1441 cagacttcat acgattctcc ggaacctgat caccaatgcg gtgaaatacc ggaaaaaaga
     1501 attgaacgac acatatgtaa aaattgtatt cggcaaaaca ggtaaccggt ttaccattac
     1561 ggtttcggac aacgggcagg ggattgccag ggaaagtatg gataaagtat ttgatatgtt
     1621 ttaccgggct acttcagccg cccccggaac cggactgggt ttatacatat gcaaagaaat
     1681 gctggaaaaa atgggagctg aattttacct ggattccact gaaggccagg gtaccagctt
     1741 tactttcaca ctggagcagg caaccccggt ggttcccgca taactattta taagtaacaa
     1801 ctactatctc cggctaatca ttcatcatta attcctgata atcaggtgct tttgcagcgg
     1861 gttcatgacc tcaaaactgg tattttccgt atttacgaaa tatctttata ccggttaagt
     1921 agcaccaata ataagtaccc tgatgctttc aaatatacat ctgagaaatc tgctcgatgt
     1981 ggatatccac ttctccaaag gttattattc taccgataat atcctggtca gtaaaggcga
     2041 ccgggtacat actacactgc tgtccattgt aatctttttt ggggtagtct ataccgtcat
     2101 tagcctgatc cagcaatatc attaccaggc tttcctcagt ttctcctgcg gggttttcgc
     2161 atttttttcc tggctgatac accgccgtgg ctatacactt ctttcaaagc tggtcaactt
     2221 cacccagctg attgccttta ttgccctgat gttttacttt ccttcctcac cctatggcga
     2281 acatatcaac gacagtgtac tggcctttta tgttccggta ttaatcggca cgctgatcgc
     2341 cttccagggc aagcaaaaag tttacggact ggtgctcgcc gtcatcgtga tattcgttat
     2401 atcatttttg attcttacag atattcatta ccccgaacac ccggtgctgc aaaactatac
     2461 aggttcgaac tatgatatgc tgtttaatat cctcggggcc gccgtggcca cattcgcaga
     2521 agtcgcttac atcctggcgc tgaatgaccg gctcaacagc agtctcgtca aaaccaatca
     2581 cgaactggat aattttatct atatcgtatc tcatgatctg cgttccccgc tgctgtccac
     2641 caaggggttg attgaccttg caaaaataaa aatcgacgaa cgggaacagg tattgaaata
     2701 cctcagtctg gcaggacaaa gcattaataa cctggaccat attatacatg aaattctcac
     2761 ttactcaaga aatgcccgta cggaattaaa ggaagaggaa tttgaattga aggatgtagt
     2821 gaaagaagtc tttgacggac tccgcttttc ggcaggatcg ggcttttcat tccttgaaga
     2881 atattccggt gaaaccaaaa tctatgcgga taaaaaccgg ctcagtaccg tgcttcgtaa
     2941 tatcatcagt aactctgtta aatataaaaa aaaggatatt gacaatccct ttgttctcct
     3001 gcgttttgaa aacaacaacc tgcactgttc attcagtata acagataacg gagagggtat
     3061 tcccctggaa agccagcacc gggtattcga aatgttttat cgtggtactt caagcgccac
     3121 cggcaccgga ctcggtcttt atatctgtaa ggaaatgctg gataaaatga atgctgtttt
     3181 cgccatttct tccaaaccgg gagaaggcac gacttttaat atacagctca acaaaacagc
     3241 aacctcatta gccaaagaaa aaaaagaacc ggcatttaat accacaaaac aatccggatc
     3301 ccccactgct tccgtaaaac tgaatgaaca gttgaatagc attcaagggt tcagttcttt
     3361 taaccaggca ggttaagccc tcaccccatt cttgcctctc gcttctcgcc tccctcacaa
     3421 aaccgcggag gacgcagaga gcgcagagaa atacaaacac cctggcagaa aagtccttct
     3481 cgcttctagc ttctggcttc tggctttctt gcacttcccg ctttaaatac gtaccttacc
     3541 tcctgagccc gtaaccaacc catgccccgc cgcctgatcc cgatattcct gatcctgacc
     3601 tgtttatgta caggtgctgc agcccagcag gcattgcctg attttacccg gatagacaaa
     3661 caggcgcgca gcatccgctt tgatggcgac ctgataaaac tcactaccac actcaccagt
     3721 ccatacccgg ataagctttc taaagtccgc tccattttta tctggatcac ggaacatatc
     3781 cgctacgact ggaaattctt taataaggat gaagaaatag agatccccga atgtaaatcc
     3841 ggacaggatt gtgatgaagt actcgccaat tgggaaaaaa aatacctgtc gaatattctc
     3901 cggaaaggaa aagcgatcag cgatggctat gcccggctct ttacccgcat gtgtgatatt
     3961 gccggactgg aagcggctac catcaacgga cataccaaga ctaaactcta ccagatcggc
     4021 aatgccggtc ctgtgaacca cagctggaat gtaatctggc tcgataccac ctggcactta
     4081 ctcgatgcca cctggtcagc cgggtatacc accgaagatg aaaacaccgg caaactcaac
     4141 ggctttgtca aacaatacaa cgactattat ttcctgaccc cctttgctga attctcgcgg
     4201 aatcactttc cacaatacac ccgctgggta ctcaagccgg gctataccaa ggacaagttt
     4261 gccgccaatc cctattttga tccggatatt ctgagcaaga tcaaattaca ttcacccacg
     4321 accggtatga tcaacggaaa aaaaggcgat accatacatt tcaaatttga atatacaggc
     4381 accattgaaa agctgcaggt aaataccaat ctttaccgca acgcggaagt ctggggttgg
     4441 gaacagatta ataaacgtaa aaaagtctgg gtgcaggata ccttcgccct gaaaagacaa
     4501 cgctatactc cttttgtgaa aacaggtaac ctgtacgaat ttgattatgt gattccggag
     4561 aactcgctct attacctgga tattcttttc g
//
LOCUS       DAOPMW010000265         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22025_length_4583_cov_4.450088, whole genome shotgun sequence.
ACCESSION   DAOPMW010000265 DAOPMW010000000
VERSION     DAOPMW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22025_length_4583_cov_4.450088"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLU11_11550"
     CDS             complement(<1..517)
                     /locus_tag="PLU11_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59805.1"
                     /translation="MYRLFLFLLLNMAMVHTAAGQTSMVDSMRHSIDQLKPGAAFINA
                     IVQLAEQNINPDTLLPYVIRAEHMAVAAGNPELNRLAERGRLNYLIRKNDHDSALVLL
                     ESMLRYYQKEKTRQPLYLSLLFTKSKVYDRAGQFTLALSNMIDVISLAEELRDTLTLV
                     QARTGIGWVQLE"
     gene            688..1479
                     /locus_tag="PLU11_11555"
     CDS             688..1479
                     /locus_tag="PLU11_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59806.1"
                     /translation="MKQKLIFFPLVMLALISCNKSDSGTTPTGLAAPPASLGLSSFYK
                     KYTDAGGIPVISSAAVEDQALLKARDIILYMISTIPAEKAKMIENKLRVGILGINERP
                     TQMPEYSDLYTAFPGTDWDNRARAYGATLQRPLTSNCEENMLCRQDIDRYKGEEILTH
                     EFAHAIHELGIRFTTPGFDTELQAAFNNAIANNLWTNTYSRTDLREYWAEGVQSYFNC
                     NKEVAAADGVHNEINTRAELQSYDPTLFNIIKRFFSAESRKHGCY"
     gene            complement(1558..2448)
                     /gene="fabD"
                     /locus_tag="PLU11_11560"
     CDS             complement(1558..2448)
                     /gene="fabD"
                     /locus_tag="PLU11_11560"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPN59807.1"
                     /translation="MKHAFIFPGQGSQFPGMGKDHYENSAFAKKLFEQANEILGFRIS
                     DLMFDGTEEDLKRTNVTQPAIFLHSVMAFKSIESVRPDMVAGHSLGEFTALVANGTLS
                     FEDALELVAVRATAMQKACEVNPSTMAAVLNLADDKVENICMEVQQETGEIVVPANYN
                     CPGQLVISGSKKGIDIACERMKAAGAKRALVLPVGGAFHSPLMLPAKEELQTKILSTK
                     FHTPTCAIYQNVAAKAVMDNEEIKQNLVDQLTGAVKWTQSVQAMIADGATRFTEAGPG
                     KVLQGLVLKINKEMQVEGVN"
     gene            complement(2535..3131)
                     /gene="folE"
                     /locus_tag="PLU11_11565"
     CDS             complement(2535..3131)
                     /gene="folE"
                     /locus_tag="PLU11_11565"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPN59808.1"
                     /translation="MAYSKIEQYDEHCTNGLIQSYKDVLGLIGEQPEREGLLKTPERV
                     AKAMQYLTQGYQLDAHAILNSAKFHEEVSEMIVVKDIEIFSMCEHHMLPFFGKAHVAY
                     IPNGWITGLSKVARVVDVYARRLQVQERLTIQIKDAIKETLNPLGVAVVIEAKHLCMM
                     MRGVQKQNSVTTTSAFDGEFQKNSTRGEFMKLIGSRLS"
     gene            complement(3142..3555)
                     /locus_tag="PLU11_11570"
     CDS             complement(3142..3555)
                     /locus_tag="PLU11_11570"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPN59809.1"
                     /translation="MSNKVAVFRKESFNAAHRLFNPAWDNATNDRIFGKCALPNYHGH
                     NYDLVVKVVGEPDPATGYVMDMKLLSDLVQQEIVERFDHRNLNLDTVEFRNLNPTAEN
                     IAVVIFNLLRPHIESRLELQVRLYETPRNFVEYPA"
     gene            complement(3611..4039)
                     /locus_tag="PLU11_11575"
     CDS             complement(3611..4039)
                     /locus_tag="PLU11_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPN59810.1"
                     /translation="MVYLTRIEHFNSAHKLYNPRWTKEKNESVFGRCANANWHGHNFE
                     LYVTIKGNPDPDTGFIFDAKQLGKLVQERIIEKLDHRNLNEDVDFMKGKMCSIENLVI
                     GIWNELQPCLPESAPLHCLKLVETAKIYVEYYGEQALPNK"
     gene            4038..>4583
                     /locus_tag="PLU11_11580"
     CDS             4038..>4583
                     /locus_tag="PLU11_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59811.1"
                     /translation="MLYFLSFPSGIRAEGRPLYFSGGAKGTRNFPFSYPDKEQISTFA
                     ETAKMNILVIAATPIEISPFLEQLRNGAITQQPDVLITGIGLTATTWSLTRQLLIKKP
                     DLVIQAGVGGCFDRSIATGTVMAIKQEAIADQSVIELNALKTLFDLNLVPQNQFPFTK
                     GWMVNKNEVLKKVKLKKVKGIS"
BASE COUNT         1191 a         1122 c         1015 g         1255 t
ORIGIN      
        1 tctccaattg tacccagcct atacccgtcc ttgcctgtac cagggtcagt gtatccctga
       61 gttcttcggc caggctgatc acatcgatca tattggataa agccagtgta aactgaccgg
      121 cccggtcata cactttggat ttcgtgaata aaagagacag gtataatggt tgccgcgttt
      181 tctctttctg ataatagcgt agcattgatt ccagcaatac caaggcactg tcatgatcat
      241 tcttccggat caaataattc agccgccccc tttctgctag ccggttcaat tccgggttac
      301 cggcagcaac tgccatatgc tccgcccgga tgacataggg caacaaggta tccggattta
      361 tattctgctc ggccagttgc actatcgcat tgataaaggc cgctccgggt tttagctgat
      421 caatggaatg ccgcatactg tccaccatgg atgtttgtcc ggctgcagtg tgcaccatag
      481 ccatattcag gagtaaaaaa aggaataacc ggtacatggg aatcagaaaa tgattttcct
      541 aaaataatca tttctcctct tgcctgtgca ggaaaaatag ccctactaca aattgagtat
      601 tgggcggtcc ggcccgatga gtcaatttca ggcctgaaaa acctcttatg ttttccttct
      661 ttcattccat gaacaaaaac aacacatatg aaacaaaaac tcattttctt tccgctagtg
      721 atgctggccc ttatcagttg caataaatcg gattccggaa ccacgcctac cggactggca
      781 gctcctccag catccctggg cctgagctcc ttttataaaa agtatacaga tgccgggggt
      841 attcctgtca tcagttcggc ggcagtggag gaccaggctc ttttaaaagc cagggatatt
      901 atactttata tgatcagtac catccctgcc gaaaaagcaa aaatgatcga aaataaatta
      961 cgggtcggca ttttaggcat caatgagcgg cccacacaaa tgccggagta cagtgatctc
     1021 tataccgcct tcccgggcac agactgggat aaccgtgccc gtgcttatgg tgccacgctg
     1081 cagcgcccgc tgaccagtaa ttgtgaagaa aacatgttgt gccgtcagga tattgaccgt
     1141 tataaggggg aggaaattct gacccatgaa tttgcccatg ctattcatga actgggtata
     1201 cggtttacca cgccgggttt tgatacggaa ctgcaggcgg cttttaataa tgccatcgcc
     1261 aataatctat ggacgaacac ctattcacga actgatttac gtgaatactg ggcagagggc
     1321 gtacagagtt atttcaattg taataaggaa gtagctgcgg ctgatggggt acataatgaa
     1381 ataaataccc gggccgagct acagagttac gacccaactc tcttcaatat catcaaacgg
     1441 tttttcagtg cggaaagccg taaacatggc tgttactaac ccaggctgaa tatttgtcaa
     1501 aaaaaaatca cccggatttt ccgggtgatt tttatatata tggttggcag acatttttta
     1561 attcacgcct tccacctgca tttctttatt gattttcagc accagtccct gcagcacttt
     1621 acccggacct gcctctgtaa aacgggtagc gccatccgca atcattgcct gtacactttg
     1681 ggtccatttt acggcaccgg tcagctggtc taccaggttt tgtttaattt cttcattgtc
     1741 catcacggct ttggccgcta cattctggta gatggcacag gtgggggtat gaaatttagt
     1801 agagagaatc ttggtctgca attcttcctt ggccggcaac atcagtgggg agtgaaaagc
     1861 accgcccacg ggtaagacca gggctctttt ggcacccgcg gccttcattc gttcacaggc
     1921 aatatcaata cctttctttg aaccactgat caccagctgg cccggacaat tataattagc
     1981 cggcacgaca atctctccgg tttcctgttg tacttccata cagatgtttt ccactttatc
     2041 atctgccagg ttgagcacgg ctgccatcgt ggatggattc acttcacagg ctttctgcat
     2101 cgccgtggca cggaccgcca ctaactcaag cgcatcctca aaactcaggg tgccattcgc
     2161 caccagggcg gtaaactccc ccagtgaatg gccggccacc atatccggcc ggacgctttc
     2221 tatactctta aaagccatta ctgaatggag aaaaatagca ggctgggtta cgtttgtccg
     2281 tttgaggtct tcctctgtcc cgtcaaacat gaggtctgaa atacggaagc ctaatatttc
     2341 gttcgcctgt tcaaataatt ttttagcaaa tgcactgttc tcataatgat cctttcccat
     2401 tcccggaaac tgggatcctt gcccgggaaa aataaacgcg tgtttcatgg tagctggtag
     2461 ttatagaaac aaaaatgcca taaaggtttt aattagaaaa atctttatgg catttccgca
     2521 aataatattc attatcagct taaacgggag ccgatcagtt tcataaactc tccccgggtg
     2581 gagttttttt ggaattcgcc gtcaaaagca gaagtcgttg taacagaatt ttgcttttgc
     2641 accccccgca tcatcataca aaggtgtttg gcttcaatga ccacggcaac tcccagcgga
     2701 ttcagggttt ccttaatggc atccttgatc tggatggtga gtcgttcctg tacctgtaaa
     2761 cggcgggcat atacatccac cacccgggct actttgctta gtccggtgat ccagccattc
     2821 gggatatagg cgacatgggc tttcccgaaa aacgggagca tatggtgttc acacatactg
     2881 aaaatctcaa tatcctttac cacaatcatc tcacttactt cttcatggaa cttagccgaa
     2941 ttcaggatcg catgcgcatc cagctggtag ccctgtgtga ggtactgcat cgctttggct
     3001 actcgttcgg gtgtttttaa taaaccttcc ctttccggct gttcaccgat caggccgagt
     3061 acatctttat aggattgaat aagcccgttg gtgcagtgtt catcatactg ttcaatttta
     3121 ctatatgcca taattccatt tttatgccgg atactctaca aagttccggg gtgtttcata
     3181 taaacgcacc tgcaattcca gtctggattc aatatgcggg cgcaataaat taaaaattac
     3241 gaccgcgata ttttcagcag tcgggttcag gttcctgaat tccaccgtat cgagattcag
     3301 gttgcggtga tcgaagcgtt ccacaatttc ctgctgcacc aggtcactca gcaacttcat
     3361 atccattaca taaccggtag ccggatccgg ctcacccacc acttttacca ccaggtcata
     3421 gttatgtccg tggtaattgg gcagggcaca ttttccaaat atgcgatcat tggtcgcgtt
     3481 gtcccatgca ggattaaaca gccggtgagc ggcattaaaa ctttcttttc ggaaaacggc
     3541 caccttatta ctcatgttac aatttatgaa atatgaacgg ctaatgcgaa agtaataaca
     3601 gggaaaagga ttatttgttc ggtaatgcct gctccccgta atattccaca taaatcttag
     3661 ccgtttccac cagtttcagg caatgcaggg gggctgattc cggcaaacag ggctgtaatt
     3721 cattccagat accaatcacc aggttttcaa tagaacacat tttccctttc atgaaatcca
     3781 catcttcatt gaggttccgg tgatccagtt tctcgataat ccgctcctgt accagtttac
     3841 ccagttgttt ggcatcaaaa ataaagccgg tatccgggtc cggattgcct ttgatggtta
     3901 catataattc aaaattatga ccatgccagt ttgcgttggc acagcgcccg aaaacggact
     3961 cgttcttttc tttagtccag cggggattat acagcttgtg ggcagaattg aaatgttcaa
     4021 tgcgggttaa gtaaaccatg ctttactttt tgagtttccc ttccggcata cgggcggaag
     4081 ggcggccgtt gtattttagc ggcggcgcca aaggtacgag aaacttcccc ttttcttacc
     4141 cggataagga acaaataagc acctttgcag aaaccgcaaa aatgaatatc ctggtcattg
     4201 ctgccactcc cattgaaatc agtcctttcc tggagcagtt gagaaacggg gccattacac
     4261 agcagccgga tgtactcatc acgggcatcg ggctgaccgc caccacctgg tcactgaccc
     4321 gtcaactgct gatcaaaaaa ccggacctgg ttatacaggc gggagtaggt ggttgttttg
     4381 accggagcat tgccaccggt acggtgatgg ccatcaaaca ggaagccatc gccgatcaga
     4441 gtgtgatcga actgaatgcg ttgaaaacgt tgtttgacct taatctcgtg ccccagaatc
     4501 agtttccctt tactaagggc tggatggtca ataaaaatga agtgctgaaa aaagtgaaat
     4561 tgaaaaaagt gaagggcatc tcg
//
LOCUS       DAOPMW010000266         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22238_length_4552_cov_5.866578, whole genome shotgun sequence.
ACCESSION   DAOPMW010000266 DAOPMW010000000
VERSION     DAOPMW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22238_length_4552_cov_5.866578"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="PLU11_11585"
     CDS             complement(<1..2405)
                     /locus_tag="PLU11_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN59812.1"
                     /translation="MRKILQLFLAFSVMLLLLPQVMQAQERTITGTIVSEDNKTPLAG
                     VTVRVKGTRKITQTDANGKFTLKVNPGEVLQITYVGYVTADIKPGDGATLGISMKTAD
                     NTMGEVVVTAMDIKRSPRSLGYSTQSIKGDEIQETQRENFLNGLQGRVAGLTLDQTSG
                     VAGSSSSIVLRGFNSLSLSNQPLYVVDGVIMDNSSLNETSSGGATNGLASDRPNRVSD
                     YQNRIADINPSDIESVTVLKGPEATALYGSQASSGAIIITTRKAKTNKLAIQYDNSFR
                     IQEVTRFPEIQNTYGGGLNGIASGSFNRFGPAYPAGTQFYDNVDNFFRTGFAQTHNIG
                     MDFGVGESKFRVSGSLFDQKGVVPNNDFKRYNFRISNTTKFWKGKADITPSFAYIKTE
                     NNKVLRSSGGYLLSLLIWPNTNDIRKFENDLSGDKLDVFEGTSANGEYDNPLWNVNNN
                     FGKDESARKTLSLGVNINPWKWLTISGRFGYDTYDQNGFLFFHPQSFYVSASSGGSLD
                     NYWRKYTGYNHTITGTVKKDLSKNLSLRVMGGTMWQDYKTEMFAVYGTNMIDTVGGIT
                     NTTGNGKMYKNGNIITDKDLAALMGNYMDSNVTKASSRVRLNRNKFGEWNYVQTRQIA
                     YFGEVALSYKNLAFINYTHRFEEASTLPKQNRKYNYPGASISLIVSDLIPALKKGNIL
                     NYWKIRTSRATTARLNSPYSTQSVFVDNLASGGGYSYGFFNNNAELEPEKQNTQELGT
                     EFRLFNSRLSVDFTYYNTLNKGQIIENFRLSYGTGFVLNTQNAGSTRNKGIEIVLDYS
                     VIK"
     gene            complement(2453..>2659)
                     /locus_tag="PLU11_11590"
     CDS             complement(2453..>2659)
                     /locus_tag="PLU11_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HPN59813.1"
                     /translation="DNDWEVVQLKKAMIESSKKVICLSIAEKINTVQPIRVCGLSEID
                     MLITELEPGNPLLKTFVDAGVHVL"
     assembly_gap    2660..2759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2760..3357)
                     /locus_tag="PLU11_11595"
     CDS             complement(<2760..3357)
                     /locus_tag="PLU11_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPN59814.1"
                     /translation="MLKKERQAYILHQVNLHNKVLSSSLCTEINVSEDTIRRDLQELA
                     EEGKVIKVHGGALSHSFNEVSFPISGVYSQNQKRLIAQKAISQIQNGMFILTSGGTTI
                     MELARTLPPQLKATFISGSIPVILEYMHHPNIEVILIGDKISKNSKITVGSEAIAKIK
                     QIKPDLCFLGTNAIDAEHGITDNDWEVVQLKKAMIESSK"
     gene            complement(3712..4203)
                     /locus_tag="PLU11_11600"
     CDS             complement(3712..4203)
                     /locus_tag="PLU11_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59815.1"
                     /translation="MKKIIVTLLVVLTTGIFYTASAQQPYTTAVGVRLSSSNAMVSNA
                     ISVKHFINDKMAVEGLLSFGDPLALGALLELHQPLSSPGLSWYYGGGAYLAFLKTVNT
                     TTQKTTTDPNFGAQGVIGLDYKFMNIPLNISLDWKPELNIVNDINFEPAAIGFTARFT
                     FGK"
BASE COUNT         1189 a          980 c          987 g         1296 t
ORIGIN      
        1 ttcttaataa cactgtagtc cagcacgatc tcaatccctt tattgcgggt agaaccggca
       61 ttctgggtat tcagtacgaa accggttcca taactcagac ggaaattttc aatgatctgg
      121 cctttgttca gtgtattgta ataggtgaaa tcaacactca gacgactatt gaacagtctg
      181 aattctgtac cgagttcctg ggtattttgt ttttccggtt ccagttctgc gttgttattg
      241 aagaatccgt aagaataacc accaccactg gccaggttat ccacgaaaac agactgggtg
      301 gaatagggag agttcagacg ggcggtggtc gcacgtgaag ttcttatttt ccagtagtta
      361 aggatattcc cttttttcag ggcagggatc aggtcactca cgatcagact gatactggcc
      421 ccgggataat tatatttacg gttttgcttg ggaagcgtgg acgcttcttc aaaacggtgt
      481 gtatagttaa taaaggccag gtttttgtaa ctcagggcta cttcaccgaa gtaggcaatc
      541 tggcgagtct gtacatagtt ccattcgcca aatttattcc ggttcaggcg tacacgggag
      601 gaagctttag tgacattact gtccatataa ttacccatga gagctgccag atctttgtca
      661 gtaatgatat ttccgttttt atacatttta ccatttccgg ttgtattggt aataccccct
      721 acagtatcaa tcatgttggt gccgtatacc gcaaacatct ccgttttata atcctgccac
      781 atggttccgc ccatcactct caggctcaaa tttttgctca ggtctttttt caccgtaccc
      841 gtgatggtat ggttataccc ggtatacttt ctccagtaat tatccagtga gccaccggat
      901 gatgctgaaa cataaaaaga ctggggatgg aagaacagaa atccgttctg gtcgtatgta
      961 tcataaccga aacgtccgga aatcgtcagc catttccagg gattgatatt cacacccagg
     1021 cttaaggtct tccgggcaga ttcatcctta ccaaagttgt tgttgacgtt ccagagtggg
     1081 ttgtcatatt ctccattcgc tgaagttcct tcaaaaacat ccagtttgtc accggagaga
     1141 tcattctcga atttccggat atcattggta ttcggccaga tcagcagact cagcaggtaa
     1201 ccaccggagc ttctgagtac tttgttgttt tctgttttga tataggcaaa ggaaggcgtg
     1261 atatctgctt tacctttcca gaatttggtc gtattggaaa tacggaagtt ataacgcttg
     1321 aagtcgttat tgggaaccac gcctttctga tcaaacaaac ttccggaaac gcggaattta
     1381 gattcgccaa ccccgaaatc cataccgata ttatgggtct gggcaaaacc ggtgcggaag
     1441 aaattgtcga cattatcata aaactgggta ccggcagggt aggcgggacc aaagcggtta
     1501 aaagaaccac ttgcaatacc attcagacca ccaccatacg tgttctggat ttcagggaaa
     1561 cgggttactt cctgaatccg gaaactattg tcgtactgga tggccagttt attggttttg
     1621 gctttgcggg tggtaatgat gatggcaccg gagctggcct ggctaccata aagcgcggtg
     1681 gcttcagggc ctttcagtac ggttacactt tcaatatcac tggggttgat atccgcaatc
     1741 cggttctggt aatcactcac ccggttcggg cggtcagaag ccagaccatt ggtagcacca
     1801 ccggaagaag tttcattcag ggaagagtta tccatgatca ctccatctac cacatataaa
     1861 ggctggttgc tgagggagag ggagttgaat ccacgtaata caatggagga tgaagaaccg
     1921 gccacacccg atgtctgatc gagggtaagt ccggctacac ggccttgcag gccattgagg
     1981 aaattctcac gctgggtctc ctggatttca tcacccttga tactctgagt ggaataaccg
     2041 agtgaccgcg ggcttcgttt aatatccatg gcggtcacca ccacttcacc catcgtgtta
     2101 tcagcggtct tcatgctgat acccaatgta gcaccatcac cgggttttat atctgcagtc
     2161 acataaccta cataagttat ctgcaatact tcaccggggt ttactttcag ggtaaatttt
     2221 ccgtttgcat ccgtctgtgt gatcttacgg gtgcctttca ccctgaccgt tactccggcc
     2281 agcggcgtct tattatcctc tgacacgatt gtgcctgtaa tggtgcgctc ctgtgcctgc
     2341 ataacctgcg gcaacaacag cagcatcaca gagaaggcca ggaataactg gagaattttt
     2401 ctcatagctg ttttttttga tataattatt aaataagcag tttacaaatt aattacaaaa
     2461 catggactcc ggcatcaacg aatgttttca acagggggtt gccaggttct aattctgtga
     2521 tcagcatatc aatttcactg agcccgcata cccttattgg ctgtaccgta ttgatctttt
     2581 ccgcgatact gagacaaatc acttttttag aagactctat catggccttc tttagctgca
     2641 ccacttccca atcattatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2761 tttagaagac tctatcatgg ccttctttag ctgcaccact tcccaatcat tatcggtaat
     2821 tccgtgttcg gcatcgattg cgttggttcc taaaaaacag agatctggtt tgatttgttt
     2881 aattttcgca attgcctcag aaccaaccgt aatcttagag ttttttgaaa ttttatctcc
     2941 gataagaatc acttcaatat tgggatgatg catatattcc aggatcaccg ggatactacc
     3001 ggaaataaac gtggccttta actggggggg caatgtccgg gccagttcca taatagtggt
     3061 tccgccactg gtcaggataa acatgccatt ctgaatctgg ctgatcgctt tttgggcaat
     3121 caatcttttc tgattttgtg aataaacccc gctgattgga aaagatactt cattaaagga
     3181 atgagagagg gcaccgccgt gtactttgat gacttttccc tcttcggcca gttcctgcag
     3241 gtcgcgacgg atcgtatctt ctgacacatt gatttctgtg cagagagaag aggataaaac
     3301 cttattatga aggttcacct gatgcagtat ataggcctgt ctttcctttt taagcatccg
     3361 tcagttttag caggactaaa ataagggtat tcctgcaaaa aaacgcagaa accgggggaa
     3421 atttgcaaaa gaaagtgact taaagcagga tactcccgat caagtcaaaa gtcataagta
     3481 aaaagtaaaa aatagccccc ttgggctgaa aaagctttaa aaggcggcaa tgttccattc
     3541 aggtaccgga ttttgttttc cagggaatta taccaggaag atccttgtca tagagtattt
     3601 accaatcaca tgctggtatt ttgacttttt ccttttgact tttgaattaa aaaagggcta
     3661 catctatgta ctaagatgta accctttacc gtcccgatcc ctaaggaaaa attacttgcc
     3721 aaatgtgaac ctggccgtaa acccgatggc tgccggttca aaattgatat cgtttacaat
     3781 attcagttcc ggtttccagt ccagtgaaat attaagcgga atattcatga atttataatc
     3841 cagaccaatc accccctgtg ctccgaaatt tggatccgtg gttgttttct gtgtagtggt
     3901 attaactgtt ttcaaaaacg ccaggtaggc accaccacca taataccagc tcagtccggg
     3961 tgaactcagc ggctgatgca attccagcaa ggctccaagg gccaacggat caccaaagga
     4021 caacagtcct tctaccgcca tcttatcgtt tataaaatgt ttaacagaaa tcgcattact
     4081 gaccatcgcg tttgaactgc tgaggcgtac cccgacggcc gtggtataag gttgttgggc
     4141 ggaagcggta taaaaaatac cagtagtgag gactacaagc aaggtgacaa tgattttctt
     4201 catgatgatt gatttgtact ttctaaaacg aaaatgatgc cagctaagtc taaaggaaat
     4261 gtaaaagtta gtcgggagtc aggagtcggt agtcgggagt caatacagct tctaaggagg
     4321 ttgaagctaa aaaaaacgat gttactcgtt ttacgaaatg cctttcgtac ttcgtacctc
     4381 gtacttaact caattgttga attttagctg aaagtagtta ttcaagatcc atttcttaga
     4441 agtctattca aacaactcat actttgtctt gggtctcctg ttgtcgagcc attgaaattt
     4501 ttcgagacgc atggaggagg gggatttgtc tttcatgggc cagatggtgc cg
//
LOCUS       DAOPMW010000267         4529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22371_length_4529_cov_12.860304, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000267 DAOPMW010000000
VERSION     DAOPMW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22371_length_4529_cov_12.860304"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..937)
                     /locus_tag="PLU11_11605"
     CDS             complement(146..937)
                     /locus_tag="PLU11_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPN59816.1"
                     /translation="MDQFQGMIAGIILFVIILFSSAIRILREYERGVIFRLGRLISGD
                     GVKGPGLILLIPFVDRMVKVSLRTVVLDVPPQDVITQDNVSIKVNAVVYFRVMEPQKA
                     VVQVENFLAATSQISQTTLRSVLGQSELDDLLSQRDKINHKLQQIIDTNTEPWGVKVS
                     NVEVKQIDLPQEMQRAMAKQAEAERERRSKVIAAEGEFQASQRLADAAKILGEQPSAL
                     TLRYLQTLREIATENNSTTIFPVPIDLLKPFLNSGGKKLEEESTK"
     gene            complement(948..2234)
                     /locus_tag="PLU11_11610"
     CDS             complement(948..2234)
                     /locus_tag="PLU11_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPN59817.1"
                     /translation="MKQFLLYGFFLFFLSGSLVAQTVISMKVEGAINPVTADFIKEGI
                     EKATQQKAACLLIELNTPGGLLKSTRNIVSYILESPVPVIVYISPAGSQAGSVGVFIT
                     LAAHIAAMTPGTNIGAAHPVTLQGQMDSIMSEKVTNDAAAFIRSIAEKRNRNIQWAEN
                     AVRRSFSYTETEALEDSAIDLIAVNVQDLLNKVDGKTILLFNGTQATLQTKTAVIEPL
                     KMNFIQQFLHTISDPTIAYILLLLGFFGILFELFIPGAILPGVIGGISLILAYYAMHT
                     LPINMAGLALILFALILFLLEIKVVSYGILAIGGVISLMIGSMMLIKSDSLLELVSIS
                     STIIYGATAVIALFFLFIIAKGIKAQKRENVTGPSALIGRTGEVTETLSPTGIVLVNG
                     ELWNSESVSGTVAVGEKVRITEVRKLKLYVESITHT"
     gene            complement(2665..>3276)
                     /locus_tag="PLU11_11615"
     CDS             complement(2665..>3276)
                     /locus_tag="PLU11_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN59818.1"
                     /translation="AVTVQTDKGTYTGKKLVITVGPWSAELVPGIQEKLKITRQVLSW
                     ISPDSLEQFTMDRFPCWMIVKPGSPGSYYGFPAMKSGEVGGPAGIKLALHHPGQVTSA
                     GTIDRSIHPADLDHLVSFAKEYIPSAGTAINDTATCMYSNSPDEHFIIDHLPGTDKQV
                     ITAWGFSGHGFKFVSVVGEVLAELVVDGRTELKIGFLSADRLG"
     assembly_gap    3277..3376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3377..3624)
                     /locus_tag="PLU11_11620"
     CDS             complement(<3377..3624)
                     /locus_tag="PLU11_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN59819.1"
                     /translation="MAQQEALLYDCIVLGVGSMGASACYHLAACGQKVLGIEQFDVLH
                     TQGAHSGHTRIIRKAYFEHPDYIPLLKRAYENWAALES"
     gene            3769..>4529
                     /locus_tag="PLU11_11625"
     CDS             3769..>4529
                     /locus_tag="PLU11_11625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPN59820.1"
                     /translation="MVNKLSPFIFMLLFTACSSESKKVKAPAFADMKWIDLTYTYDST
                     TLYWPNNVKNFQHSTEAEGKTALGFYYSSYSICTPEHGGTHLDAPIHFAENKMTVDEL
                     PLSSLTGDAVVIDVSKKALANRDYLISIADVEEWEKTNGTFPENTIVLFKTGYGQFYP
                     NREKYFGSAKTGPEAIPELHFPGVDPVTTEWLVNKKKIKAMGLDTPSIDYGQSKDFKT
                     HQILLGANKPGFENVANLDQLPAKGIYVVALPMKIG"
BASE COUNT         1190 a         1097 c          979 g         1163 t
ORIGIN      
        1 aaatacaaga tttctcgctg cgctcgaaat acccgtgata gatcaaaggg aagcgagcaa
       61 ccagaagcta gaagcaatcc taaagcagga tgaacctgct agtcaagcca tctatctcag
      121 gaggccactt cgtacttcgt acctcttact tcgtactttc ttcctccagc ttcttccccc
      181 cactattcaa aaaaggtttc agcaaatcaa tcggcacggg gaatatcgtg gtggaattat
      241 tctccgtggc aatttccctt aatgtctgca ggtaccgcag ggttaatgca ctgggttgtt
      301 cgccgagaat cttggcggca tcggccagtc gttgggaggc ctggaattct ccttcggcag
      361 caataacctt gctccggcgt tctcgttccg cttcggcctg tttggccatg gcccgttgca
      421 tttcctgtgg caggtcaatc tgcttaactt ccacattcga tactttcaca ccccagggtt
      481 ctgtattggt atcaatgatc tgctgcaact tatgattgat cttatcccgc tgtgacaaca
      541 ggtcatccag ttccgattgt cccaatacac tacgcaaggt tgtttgagaa atctgcgatg
      601 tagccgccag aaaattctcg acctgcacca cggctttctg tggttccatg accctgaaat
      661 acaccaccgc atttacttta atcgaaacgt tgtcctgggt gatcacatcc tgaggaggta
      721 catccagtac cacggtccgt aaactgactt ttaccattcg gtcaacaaac ggtatcagca
      781 ggatcagtcc ggggcctttt actccatcac cactgatcag ccgacccaac ctgaaaataa
      841 cgccccgttc atattcccgg agaatgcgga tggcactgga aaataaaatg attacaaata
      901 aaataatacc ggcaatcata ccttggaatt ggtccataaa aatttattta agtgtgagta
      961 atagattcaa catataattt cagtttcctg acttccgtaa tacgtacctt ttcaccaacg
     1021 gctactgttc cggataccga ttcactgttc cataattccc cgtttactaa aacgattccg
     1081 gtgggagaaa gcgtttctgt tacttcgccc gtacggccaa tcaaggcaga tggaccagtc
     1141 acattttccc gtttttgtgc ttttattcct tttgctatta taaataagaa gaacagggcg
     1201 atgacggccg tagccccata tataatggtg ctggatatac ttaccagttc gagcagggaa
     1261 tcagatttta tcagcatcat ggatccgatc attaaggaaa taacaccgcc gatggcgagt
     1321 atgccatagc tgaccacttt gatctctaat agaaaaagga tcagggcaaa caggattaat
     1381 gccagtccgg ccatattgat cggcagggta tgcatggcat aataggccag gatcagactg
     1441 atgcccccga tcaccccggg taggatggca cctggaataa agagttcgaa caggatacca
     1501 aaaaaaccaa gcagcagtaa gatatacgca atagtaggat cactgatcgt atgcaaaaac
     1561 tgctgaatga aattcatctt caacggctca atcactgccg ttttagtttg cagtgttgct
     1621 tgtgtgccgt taaacagcaa aatagtttta ccatccacct tgttcagcag gtcctgcaca
     1681 ttcaccgcaa tcagatcaat ggcggaatct tcaagcgctt ctgtttctgt ataggaaaaa
     1741 cttcgtcgga ccgcattctc cgcccactgt atattacggt ttcttttctc cgcgatcgaa
     1801 cggataaatg ccgccgcatc attggtcact ttttccgaca taatgctgtc catctgtccc
     1861 tgcagcgtaa ccggatgagc ggccccgata ttggttcccg gtgtcatggc cgcaatatgg
     1921 gctgccaacg tgataaacac accaaccgaa ccagcctgtg aaccagccgg ggaaatgtaa
     1981 acgatcaccg gcactggcga ctcgagtata tagctgacga tattgcgggt tgatttcaat
     2041 aatccgccag gcgtattcag ttcgatcagc aggcaagctg ctttttgttg ggttgctttt
     2101 tcaatgcctt ctttaataaa atcagcggta acgggattaa tagcaccctc cactttcatg
     2161 gaaatcactg tttgtgcaac cagtgagcca gaaagaaaaa acaggaagaa accatatagt
     2221 agaaactgtt tcataacacc agccggatct gcagcggtcc ggtggttgag ttggtagtta
     2281 cgtaatttac tgaattttat cgagagaagc gagaaactag aagcgagaag gggcctccta
     2341 agggctgtta cgtgacatcc ggcaactgac aactgatacc tttgctgctt cctaattccc
     2401 tctattaata caaggttcct ccttcgtcgg aatgacgagg cttgagggga aaaaagggaa
     2461 atacaagatt tctcgctgcg ctcgaaatag caatagcaat ttgttgttct agaagttttc
     2521 ctcaggtggg actgtttcct aacacctaac agctaacaac gaacaccttc tctcaggaga
     2581 ggctgtttcc taacacctaa cagctaacaa cgaacacctt ctctcaggag gggctgttcc
     2641 cttctcgctt ctagcttctt gcttctagcc taacctatca gcgctaagaa aaccgatctt
     2701 caactctgtt ctcccatcta ctacaagctc cgctaacacc tcccctacta cggaaacaaa
     2761 cttaaacccg tgcccgctga agccccaggc agtaatcacc tgtttatcag tgccggggag
     2821 atgatcaatg ataaaatgtt catcgggtga attgctgtac atacaggtgg ccgtgtcgtt
     2881 gatggcagta ccggctgatg gaatatactc ctttgcaaac gatacgagat gatcaagatc
     2941 tgccgggtgt atcgaccggt caatggtgcc agccgaggtg acttgtcccg gatgatggag
     3001 ggccagtttg ataccagccg gtccgcccac ttctccggat ttcatggcgg gaaaaccata
     3061 ataggatcct ggactgcctg gtttcacgat catccagcat ggaaaacgat ccatagtaaa
     3121 ttgttccaat gaatcaggcg atatccaaga aagcacctgc ctggtgattt ttaatttttc
     3181 ctgtataccc ggcactagtt ccgcagacca gggcccgaca gtgatcacca gttttttacc
     3241 agtataggtt cctttatctg tttgcactgt aactgcnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnatgc tttccaacgc tgcccagttt tcataagccc gcttcaacag
     3421 cggaatataa tccggatgtt caaaataggc tttgcggata atgcgggtat gtccactatg
     3481 cgccccctgt gtatgcagca catcaaactg ctcaatcccc aatacttttt gtccgcaggc
     3541 cgccaggtga taacaggcag aggcgcccat ggagccgacg ccgagaacga tgcagtcgta
     3601 taggagggct tcttgttgtg ccatggaatt tggttggagt tgtaagtggt aagttatacg
     3661 ttttaagtat ggaattcggt ctgaataatg ggagtttcgg taaattaggg aattctctgc
     3721 tttccggttt tttaattttt acttttgact ttaaactttc tataaactat ggtaaataag
     3781 ttatcgccgt tcatcttcat gctgcttttc accgcctgca gttcagaaag caaaaaagtg
     3841 aaagcccccg cttttgctga tatgaaatgg atcgacctca cttatactta cgacagtacc
     3901 actttgtact ggcccaataa tgtgaagaac ttccagcatt ccacagaagc agaagggaaa
     3961 acagcgttgg gtttttatta ttcctcctac tccatctgta cccccgaaca tggcggtacc
     4021 cacctggatg caccgatcca ttttgcggaa aataaaatga ccgtggatga actgccgctc
     4081 agcagtctga ccggtgatgc cgtggtaatc gatgtatcca aaaaagcatt ggccaaccgt
     4141 gattatctca tcagcatcgc cgatgtggag gagtgggaaa aaaccaacgg tacattcccg
     4201 gagaatacca tcgtgttatt caaaaccggt tacgggcagt tctaccctaa ccgtgaaaag
     4261 tattttggaa gtgccaagac aggtcccgaa gccatccccg aattgcattt ccccggtgtg
     4321 gaccccgtaa ccaccgaatg gctggtcaat aaaaagaaga taaaagccat gggactggat
     4381 acaccaagta ttgattacgg ccagtcaaaa gattttaaaa cgcaccagat acttctcggt
     4441 gccaacaaac ccggttttga aaatgtcgcc aacctcgatc agctgccggc caaaggcatt
     4501 tatgtcgtag cattgcccat gaagatcgg
//
LOCUS       DAOPMW010000268         4474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22779_length_4474_cov_6.349853, whole genome shotgun sequence.
ACCESSION   DAOPMW010000268 DAOPMW010000000
VERSION     DAOPMW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22779_length_4474_cov_6.349853"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLU11_11630"
     CDS             <1..554
                     /locus_tag="PLU11_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPN59821.1"
                     /translation="EVTKEMRYKAKSVNFGIIYGQGAFGLADNLGISRTEAKEIIDNY
                     KKEFPGIQQYMDTTINFAREQGFVETLMGRKRWLRDINSSNFTVRGFAERNAINSPIQ
                     GSAADMIKLAMQKVHAAMKKANMKSRMILQVHDELVFDALKTEVNELKPLILENMETA
                     LLLPNEVPVIAECGEGNNWLEAH"
     gene            complement(602..3019)
                     /locus_tag="PLU11_11635"
     CDS             complement(602..3019)
                     /locus_tag="PLU11_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN59822.1"
                     /translation="MKRIIQPILILLLIVQAVPAQVWQPLGPDDTNWPYTGGINYLSA
                     AISGQGVPYVAYMDRGLGDKASCRKFQGGQWVDVGQRGFSEGAVAFTSIAIKDNGVPY
                     VVYQENNQKASVKKFISGNWVNVGQPGFTESYALNTSIVLDDNGTPYVVYIDGYGPMA
                     GAVTVKKFTTGQWTTVGQRQFSAGKAEAPSMAIDNNGTPYVVYQDWTANYKTVVKKFT
                     GSNWAEVGNIDHFSYKETKIAINTNGVPFIAYQDETAGLIRVKKFNLGIWKDIGTSGL
                     PAVVNGPVHLAINTPGTPYVSYVSYSPESNYVKKFTGGNWVEVGVPGEAGALIGFDGQ
                     HVPYVFSETVRKLTNGQWKDLGTRYGFSAGSAANVAAVMDGNGTPYVVYRDLANNGKA
                     TVMKYENGSWMNTGSAGFSADSAWYTSLAIKNNGVPFVVYKDKGSNGRATVKKFSNYS
                     WRNVGLPGFSAGEVAYTSIVLNKNAVPYVVYKDLAYNGKATVRKFTDGSWKNVGPPGF
                     TQAAVTNTLIRMECGTVPWIVYLDQLSGNVELRKFQQGNWVEIPSPGQSSSDVDMVIN
                     EWGTPYVFFREYAYPRPLTVKYLTGDTWTTAGPPVETAENFEFFNQQILLNAGSDPYV
                     LYTKTGPVQPESFVTREISVKYFNGSDWADIWPAGLGGIHVSAATFAFKPTGQLFVAY
                     HQNNGRGMFAKIAEAPYYNPASYARNSMSQTGPEPSTSDNEISMHVYPNPVVTNAVVY
                     ISLNNASSVALRLSTLKGQLIWAKEVSLSQGLSVLDLPVSQLNGGVYLLTAKTTDGRI
                     TTVRIIK"
     gene            3205..3891
                     /locus_tag="PLU11_11640"
     CDS             3205..3891
                     /locus_tag="PLU11_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HPN59823.1"
                     /translation="MSSYLRLMEPVIYYCYDAYCGWCYGFSPVIKKIADEWKDRMRFE
                     VLSGGMIPKEAARPVSSIAGYIVEAYKTVEERTGIRFGEDFLWHMRNPDQSDWFMHSE
                     KAAAALCIFKEIHRDLQVSFAADLQYSLNYEGRDLTDDEAYRHLLVKYGIPEDSFYSR
                     LNSEEYIEKARYEFTLCKQLQVTGFPQVLLQVADQKFYLLSKGYTDYETLNNRFLSVW
                     KEINKDTGNK"
     gene            3904..>4474
                     /locus_tag="PLU11_11645"
     CDS             3904..>4474
                     /locus_tag="PLU11_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPN59824.1"
                     /translation="MILTVTLNPCIDKSSRVEKMKPESKLRCAEVVNEPGGGGINVSK
                     ALKKLETSSVALFPAGGHNGDMLCSLLKKEGILFHAVDTKVETRENWIVLETSTNSQY
                     RFTFPGREVLEETVKTMVDQIRSFAPSFVVASGSLPPGLPDYFYGLIVKNAAAVGARC
                     IVDTSGPALQALKGKHAFLIKPNIGELCKM"
BASE COUNT         1201 a         1046 c         1025 g         1202 t
ORIGIN      
        1 aagaagtaac caaagagatg cgctacaaag caaagagtgt aaactttggt atcatttacg
       61 gacaaggagc atttggcctg gccgataatc tgggtatttc ccgtactgaa gccaaagaga
      121 ttattgataa ttataaaaag gaatttcccg gtatccagca atacatggat accaccatca
      181 attttgcccg ggagcagggt tttgtggaaa ccctgatggg acgtaagcgc tggttacgcg
      241 atatcaactc ttccaatttc acggtccggg gatttgcgga acgaaacgct atcaattccc
      301 cgattcaggg ttcggcagct gatatgatca aactggcgat gcagaaagta catgcggcga
      361 tgaaaaaggc aaacatgaaa agccggatga ttttgcaggt gcatgatgaa ctggtatttg
      421 atgcactgaa aacagaagtg aatgaactga agcccttgat actcgaaaat atggagacgg
      481 ccctgctgct acccaatgaa gtgccggtga ttgcagagtg cggcgaaggg aataactggc
      541 tggaagcgca ttaaaactaa aagcgggccg gtggtccgct tttagtttta ctattactgc
      601 gttattttat gatccggaca gtggttatcc ggccatccgt tgtttttgct gtaagcaggt
      661 atacacctcc gtttagctgg ctgactggta aatcaagtac agataatccc tgggacaggg
      721 atacttcttt ggcccatatc aattgacctt ttaaagttga caggcgaagc gcaacactgg
      781 atgcattatt caatgaaata taaacaacgg catttgtcac aaccggatta ggatatacat
      841 gcattgatat ttcattatcg ctggttgacg gctcagggcc agtctggctc atactgttcc
      901 tggcgtatga tgcagggtta tagtatggtg cttctgctat tttcgcaaac atcccccggc
      961 cattattttg atgataggca acaaataact gaccagtggg tttaaacgca aaagtggcgg
     1021 cggatacatg tatacctccc aggccggccg gccagatatc cgcccaatca gacccgttaa
     1081 aatatttcac acttatttcc cgggtgacaa atgattctgg ctgtaccgga cccgtctttg
     1141 tatataagac atatggatcg cttccggcat tcaggagaat ctgttgattg aagaattcaa
     1201 aattctcagc agtttcaacc ggcgggcccg ctgtagtcca ggtatcaccc gttaaatact
     1261 tcactgttaa aggccgggga taagcatatt cacgaaaaaa cacatagggg gttccccatt
     1321 catttattac catatccaca tctgaggatg attgtccggg tgatggaatc tcaacccagt
     1381 ttccctgttg aaattttcgc agttccacat tcccgctaag ttggtctagg tatacaatcc
     1441 agggcactgt tccacattcc atccggatca atgtatttgt tacagccgcc tgggtaaaac
     1501 cgggaggtcc tacatttttc caacttccat ctgtaaattt tctgaccgtt gctttcccgt
     1561 tatatgccaa atccttgtaa accacataag gcaccgcatt tttattcaaa acgatcgaag
     1621 tatatgccac ttcaccggct gaaaagccag ggagtccaac atttcgccag ctgtaattgc
     1681 tgaatttttt tacagtggca cgcccgttag accccttgtc tttatacacg acaaaaggca
     1741 ccccgttatt tttaatggcg agggaagtat accaggcaga atcagctgaa aatccggcgc
     1801 tccctgtatt catccagctg ccgttctcgt atttcattac ggtggctttc ccgttatttg
     1861 ccaggtcccg gtaaactaca taaggtgtac cgttgccatc cattacggca gctacatttg
     1921 ctgccgaccc tgcagagaat ccataccggg tacccaggtc tttccattgc ccgttagtca
     1981 gtttgcggac agtttctgaa aacacgtaag gaacatgctg accatcaaaa ccgattaaag
     2041 cacctgcttc acccggcacc cccacttcga cccaattacc tcctgtaaat ttttttacat
     2101 agttagattc aggggagtag gatacataag atacataagg tgtacccggc gtattgatag
     2161 cgagatgaac aggaccattt accactgcag ggagaccgga tgtcccaata tctttccata
     2221 ttcccaaatt gaatttttta acccgtataa gaccggcggt ttcatcctga tatgctataa
     2281 aaggcacccc attagtatta attgcaatct ttgtttcctt ataactaaag tggtcaatat
     2341 tacccacctc cgcccagttg cttccggtaa attttttaac caccgtttta taatttgctg
     2401 tccagtcctg gtagactaca tagggtgtgc cgttattatc aatggccatt gaaggtgctt
     2461 ccgccttgcc agctgaaaat tgtcgttgtc cgacagtagt ccattgaccc gtggtgaatt
     2521 ttttaaccgt aactgcgccg gccattggcc catatccatc aatatataca acataaggag
     2581 ttccattatc atcaaggacg atggacgtgt ttaatgcata actttctgta aaaccgggct
     2641 ggccaacatt cacccagttg ccagaaataa attttttaac agaggccttc tgattgtttt
     2701 cctggtatac cacgtatgga acaccattgt ctttaatggc gatggaagta aatgcaacgg
     2761 ccccttctga aaacccccgc tgtcccacat ccacccattg gcctccctgg aattttctgc
     2821 agctggcttt atcgcccaac cccctgtcca tataggccac atacggaacg ccctgaccgc
     2881 tgatagccgc tgataagtaa ttgatgcctc cggtataggg ccagttcgta tcatccggtc
     2941 ctagcggctg ccatacctgt gccgggactg cttgtacgat caacaacaga atcagtatgg
     3001 gttgaattat tcttttcata atcacctcct tttaagcctt ctctgaagtt agtatcaacc
     3061 tgccggggtg aatatatgat acaggtcaca ttgacgggtg acaggaatca aatcggccat
     3121 cttaatcacc tgagaatgtt tcgtatgccg gaaataattc caaaacatga ccgatacagg
     3181 agatggtttg gtaattaata ttctatgtca tcttatcttc gcctgatgga gccggttatt
     3241 tattattgct atgatgccta ctgcggctgg tgctatggct tcagtccggt tattaaaaaa
     3301 atcgcggacg agtggaaaga ccggatgaga tttgaagtac tctccggggg tatgatcccc
     3361 aaagaagcag ccaggcctgt aagttcaatt gccggctata tcgtggaggc ctataaaacc
     3421 gtggaggaac ggaccggcat ccgtttcggg gaggattttc tctggcatat gaggaatcca
     3481 gaccagagtg attggtttat gcattcagaa aaagcggcgg cggccctttg tattttcaag
     3541 gaaattcacc gggatctcca ggtgtctttt gcagcagacc ttcagtacag tctgaattat
     3601 gaggggagag acctgaccga tgatgaagcg taccgtcatt tactggtgaa atacggcatt
     3661 ccggaagaca gcttttactc ccggctgaac agtgaggagt atatcgaaaa agccaggtat
     3721 gaatttacgc tctgcaaaca actgcaggtg accggcttcc cgcaggtgtt gctgcaggtt
     3781 gccgaccaga aattctactt gttgtccaaa ggctatacag attatgaaac attaaacaac
     3841 cggttcctgt ccgtatggaa agagattaat aaggataccg gaaacaaata aaatattttt
     3901 cgcatgatct taactgtaac actcaatccc tgtatagata aaagttcccg ggtggaaaaa
     3961 atgaaacccg aaagcaaact gcgttgtgct gaagtggtga atgagcccgg cggcggcggc
     4021 atcaatgtca gcaaggccct gaagaaactg gaaacctcct cggtggccct ttttccggca
     4081 ggcggacaca acggcgatat gctttgttct ttattgaaaa aagaaggcat tctctttcat
     4141 gcggtggata ccaaggtgga gaccagggaa aactggattg tactggaaac ttccaccaat
     4201 agccagtacc gctttacttt tccgggtcgc gaagtgctgg aagaaaccgt aaagaccatg
     4261 gtggaccaga tccgctcttt tgcgcccagt tttgtggtgg ccagtggcag tctccctccc
     4321 ggtcttcctg attatttcta tggactgatt gtaaaaaacg cagcggccgt gggtgcccgt
     4381 tgtattgtag ataccagtgg ccctgcgctg caggccctga aaggcaaaca tgcttttctg
     4441 atcaaaccca atatcgggga actctgtaaa atgc
//
LOCUS       DAOPMW010000269         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22840_length_4465_cov_9.189796, whole genome shotgun sequence.
ACCESSION   DAOPMW010000269 DAOPMW010000000
VERSION     DAOPMW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22840_length_4465_cov_9.189796"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLU11_11650"
     CDS             complement(<1..603)
                     /locus_tag="PLU11_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPN59825.1"
                     /translation="MKEKFADFIAKSTVKAADRDHRRKINFNIGKYNSVVPQGKMQFT
                     DLHLARERAKNIKWKAISTLDTQLEDFEAAITKRGAKVIWAENAAEALAAVKKICEEK
                     NCKTLVKSKSMVTEEIHLNEFLEKNGIESVETDLGEYIQQLDGEPPYHIVTPAMHKSK
                     EDVAKLFSHKLGTDPKATPEQLTLVARKVLREKYVQAEVGV"
     gene            complement(613..1218)
                     /locus_tag="PLU11_11655"
     CDS             complement(613..1218)
                     /locus_tag="PLU11_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPN59826.1"
                     /translation="MIVIDNILVSEDVVDKRFVCDLNKCKGGCCEEGDAGAPVDEDEM
                     QIMREVYDKVKHLLPADAIKEIDRTGLFAKDDEFGWVTNTIGSNKGICVFAFHDEQRI
                     IKCAFEQAYRDGLISWKKPISCHLYPITAKKGKNGDYERVNYEPREVLCKPGCNLGEE
                     LKVPVYQFLKEPLIRKYGEDFYNALDTIAKGEWEEIDFKEK"
     gene            complement(1348..3141)
                     /locus_tag="PLU11_11660"
     CDS             complement(1348..3141)
                     /locus_tag="PLU11_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59827.1"
                     /translation="MNRLRRLIPVILLLIVSLSARSQEAAAVDSLRMAMAKANTLEEK
                     FPLMEELSRVLMNVNPAQSDEVGSQLITLAEESRDRKYMFKAYLSNGTRCSYMASQKA
                     YSDKSLSFYNKALELARQNRLTEETAIAQQHLALFYLMIPDKEKAFAYITQASSQTAT
                     LPNDSLRAESFNIFGQVYQAKNEYIMALRSYLNGLRLAEEIKNPALVRNCYIYLSDFY
                     SDIEDYDKSIDYMTLAYKKLDQINEKNVAYQKVIYTNNLGNLFAKKKNYDIAISYFER
                     SIRMADSLKFSTLKIPGYISLLNQYLRIDEPQKALNYLNSKEGDELKKYLTNFGMAPV
                     IDQAYGVVYTGLGRYDSAGILLEKAGPMFENSGNDYNKVGFLAQLASYYNKSGNYKMA
                     IEKYLLVKEFSERNGWLENVEKASKYLDTLYRKTGNLQESGKYNALYYQYKDSIKTLK
                     RENEMRKEEADDEQQRLLKSQKEAEELKKRRNNIQYLAIVIGIISLFLSLVVLGMFKV
                     SAGVIKAIGFFVFLMFFEFIFLIFKKNIYEFTKGEPWKDLAFMIALAAILVPLHHWLE
                     HKVLHYLTSHNRLTAAGHQIRRRIFGRGDAE"
     gene            complement(3242..3880)
                     /locus_tag="PLU11_11665"
     CDS             complement(3242..3880)
                     /locus_tag="PLU11_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59828.1"
                     /translation="MIRMTRQRLSYLLCAMLLASFSLLSCNSDYSVGKKKGYFRISFP
                     EKKYQVFDQPGYPYTFEYPVYATVTRDTTFFDDKAGDWWINIDFPRFEGRIHVSYKPI
                     NAENRFDSLVSDGFKMAFKQHVDVSTGINDSLIQTPNGVEGIYFSLGGNTATANQFFL
                     TDSTRHFLRGALYFNAAPNADSLGIVNDFLKQDLKQLINTLRWVPVGDNTNR"
     gene            complement(3877..>4465)
                     /locus_tag="PLU11_11670"
     CDS             complement(3877..>4465)
                     /locus_tag="PLU11_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPN59829.1"
                     /translation="RVEELHYSRLPVYKDSLDEVVGILNTKDLIPYLNEGELFDWHSL
                     MRQPYFVPEPKLIEDLLKEFQQKRTHFAVVVDEFGGTSGIVTMEDILEEVIGDIRDEF
                     DEEENDSYKLDDYNYIFEGKIMIHDMCRIMKLPMNTFDHIKGESESLAGLVLELAGEL
                     PTVDWVIPCGDFEFAIMQAENNRIQLVKVTIKPKQ"
BASE COUNT         1074 a         1115 c          971 g         1305 t
ORIGIN      
        1 aacccctact tctgcctgca cgtatttttc gcgtaatact ttccgtgcca ccagggtcag
       61 ttgttccggc gtggctttgg ggtcggtacc gagtttatgt gaaaaaagtt tcgccacatc
      121 ttccttgctt ttgtgcatgg cgggcgtcac aatatgatag ggaggctcac cgtccagttg
      181 ctggatatat tcaccaaggt cggtctctac tgattcgatg ccgttttttt ccaggaattc
      241 attcaggtga atctcttccg tcaccatact cttgctcttc accagggtct tgcagttttt
      301 ctcttcgcag atctttttca ccgctgctaa tgcttctgct gcattctcgg cccagattac
      361 tttagcccct cttttagtaa tagccgcttc aaaatcctcc agctgggtgt cgagggtgga
      421 gatggctttc catttaatat tcttggctct ttcgcgggcc aggtgcagat ccgtgaactg
      481 cattttgccc tgcggcacca cggaattgta tttgccgata ttgaaattga ttttccggcg
      541 atggtcacgg tccgccgctt taacggtgct tttggcaata aaatcagcaa atttctcttt
      601 cacgtgacag gtttattttt ctttgaaatc aatttcctcc cattcaccct tggcaatggt
      661 atcaagggca ttgtaaaaat cttcgccata cttgcgaatc agtggctctt tcaggaactg
      721 gtacacgggc acttttaatt cctccccgag attacaaccc ggtttgcaca atacttcccg
      781 cggttcgtaa tttacccgtt cataatcccc gttcttccct ttcttggccg tgatcgggta
      841 taaatggcag ctgatgggtt tcttccaact gatcaatccg tcccggtagg cctgttcaaa
      901 agcgcatttg ataatccgtt gttcatcatg aaaggcaaat acgcagatac ctttgttact
      961 gccgatggta ttggttaccc agccaaattc gtcgtctttg gcaaacaggc cggtgcggtc
     1021 aatctctttt atggcatccg ccggcagaag atgtttcact ttatcatata cttcccgcat
     1081 aatctgcatc tcatcttcat ccaccggggc cccggcatca ccttcctcgc agcagccacc
     1141 cttgcattta ttcagatcgc agacaaagcg tttgtccacc acatcttcac tcaccagtat
     1201 attatcgatt actatcaagc gttaaagttt tgccccaaag gtacaccagt tgttggtgaa
     1261 aacaccaaca actggcaggg tatatcccgt aaatgacgta gccagtagtt ggtgttatca
     1321 cccgccagta gttggtgtta tcaccaacta ctcagcatcc cccctcccga atatcctcct
     1381 ccgtatctga tgccccgccg ccgtcagccg gttatgggag gtcaggtagt gcagaacttt
     1441 atgttccagc cagtggtgca ggggcaccag gatcgcagcc agggcgatca taaatgccag
     1501 gtccttccag ggctcaccct tagtgaattc ataaatattc ttcttaaaga tcaggaagat
     1561 gaactcgaag aacatcagga aaacaaaaaa tccaatggcc ttgattactc cggcagacac
     1621 cttaaacatc ccgagaacca ccagggataa aaaaagactg ataatgccga ttacgattgc
     1681 gaggtactga atattgttcc ttctcttttt caactcctcc gcctctttct gcgatttcag
     1741 caggcgctgt tgttcatcgt ctgcctcctc ttttctcatt tcattttccc ttttcagggt
     1801 tttgatactg tctttatatt gataataaag ggcattgtat tttccggact cctgtaaatt
     1861 gccggtttta cgataaagcg tatccaggta ttttgaggct ttttccacat tctccagcca
     1921 gccattccgc tcactgaatt cttttaccag gagatatttc tcaatagcca ttttgtaatt
     1981 tcctgactta ttgtaatagg aagccagctg ggccaaaaac cccactttat tgtaatcgtt
     2041 accactgttt tcaaacatcg gacctgcttt ttccaataaa ataccggcag aatcatagcg
     2101 gcccagacct gtatatacca ccccgtaagc ctggtcgatc accggggcca tcccgaaatt
     2161 ggtcaggtat ttcttcagct cgtcgccttc ttttgaattc aggtaattca gtgccttttg
     2221 tggctcatca atccgcaggt actggttgag caggctgata taacccggaa ttttcagggt
     2281 agagaatttc agggagtccg ccatccggat cgaccgttca aaataactga tagcgatatc
     2341 ataattcttt tttttcgcga agagattgcc caggttatta gtataaatga ctttctgata
     2401 agccacattt ttttcgttga tctgatccag ctttttatag gccagggtca tatagtcaat
     2461 cgatttgtca taatcctcaa tatctgaata gaaatcggag aggtagatat aacagtttcg
     2521 caccagtgcg ggattcttta tttcctccgc cagccgcagg ccattcaggt aactgcgcag
     2581 ggccattata tattcgtttt tggcctgata cacctggccg aaaatattga aggattctgc
     2641 ccgaagacta tcattcggca aggtggcagt ctgggaagaa gcctgggtga tataggcaaa
     2701 tgctttctcc ttatccggaa tcatgaggta gaacaaagcc aggtgttgct gagcgattgc
     2761 ggtttcttcc gtcaaacggt tttgcctggc cagctccagt gctttattgt aaaaactgag
     2821 ggatttatct gagtaggctt tctgactcgc catataactg catcgggttc cgttagagag
     2881 ataggcctta aacatgtatt tacgatcccg ggattcttct gccagcgtaa ttaattgtga
     2941 tccgacctca tccgactgtg ccggattcac attcatcagc acccggctga gctcttccat
     3001 caggggaaat ttttcctcaa gcgtatttgc cttggccatt gccatgcgga gtgaatccac
     3061 tgctgccgct tcctgggaac gggcggacag ggatacaatc aataggagta taacggggat
     3121 gagtctgcgt aaccgattca tgggtatata tcttgttggt gaaaacacca acaagatact
     3181 gcatctggtt ggtgttttca ccaactggtt acctgttggt gttttcacca actggactcc
     3241 actacctgtt ggtgttatca ccaactggta cccagcgaag cgtgttgatc aattgcttaa
     3301 ggtcctgttt caggaagtcg ttgacgatac ccagcgagtc agcattgggc gctgcattga
     3361 agtagagagc gccccgcagg aaatgacggg ttgaatcggt caggaaaaac tggttggcgg
     3421 tggcggtatt tccacccagt gaaaagtaaa tgccctccac cccattgggt gtctggatca
     3481 ggctgtcatt gattccggtg gatacatcca cgtgctgttt aaaagccatt ttaaaaccat
     3541 cgctcaccag cgaatcgaaa cggttttccg cattgatcgg tttatagctt acatggatcc
     3601 gtccttcaaa ccggggaaaa tcaatattaa tccaccagtc acctgccttg tcatcaaaga
     3661 aagtagtatc ccgggtcacc gttgcataca ccgggtattc aaacgtgtag ggatagccgg
     3721 gttggtcaaa tacctggtac tttttttcgg ggaagctgat ccggaaatag cctttctttt
     3781 tcccaacgga ataatcgctg ttgcaggaca aaaggctgaa tgatgccaac agcatggcac
     3841 aaagaagata gctaaggcgc tgacgggtca tccggatcat tgtttcggtt taatggtcac
     3901 tttgactaac tggatacggt tattctctgc ctgcataatg gcaaattcaa aatcgccaca
     3961 ggggatcacc cagtccaccg tgggcagttc gccggccagt tccagtacga gaccggccag
     4021 tgattcgctt tcccctttga tatgatcaaa ggtattcatg gggagcttca taatccggca
     4081 catatcgtgg atcatgatct tcccttcaaa aatataattg tagtcatcga gtttgtagct
     4141 gtcattttcc tcctcatcaa attcatcccg gatatccccg atcacttctt ccaggatatc
     4201 ttccatcgta acgatcccgc tggtaccgcc gaattcatcg acaaccactg caaaatgcgt
     4261 tcttttctgt tgaaactctt tcagcaggtc ttcaatcagt ttaggttccg gtacaaaata
     4321 aggctgccgc atcagggaat gccagtcaaa aagctcccct tcattgagat aaggaatcag
     4381 gtccttggta ttcaaaatac ccaccacttc atccagactg tctttataaa caggcagtcg
     4441 ggaataatgc agttcttcca cccgg
//
LOCUS       DAOPMW010000270         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22841_length_4465_cov_9.051247, whole genome shotgun sequence.
ACCESSION   DAOPMW010000270 DAOPMW010000000
VERSION     DAOPMW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22841_length_4465_cov_9.051247"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLU11_11675"
     CDS             <1..190
                     /locus_tag="PLU11_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPN59830.1"
                     /translation="DYLARITLLKRMGQVHPVVTVLGVIVGLGLFGFIGLIFGPLLVN
                     YIILLFRIYCNEFILEKK"
     gene            complement(294..1646)
                     /gene="gdhA"
                     /locus_tag="PLU11_11680"
     CDS             complement(294..1646)
                     /gene="gdhA"
                     /locus_tag="PLU11_11680"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPN59831.1"
                     /translation="MITTKKSMADKFMQLMKKRNPDQPEFLQAVSEVAQAIIPYIEEH
                     PQYKKANIMERLTEAERIIIFRVPWVDDAGNFRVNRGYRVQMNNAIGPYKGGLRFHPN
                     VSLSILKFLAFEQVFKNSLTTLPMGGAKGGSDFDPKGKSDNEVMRFCQSFMTELYRHI
                     GNDTDIPAGDIGVGGREIGFLFGQYKRLSNEFTGVLTGKGFTYGGSLIRPEATGYGLL
                     FFVDEMLRTKGETLKNKRVVVSGSGNVAQYAIEKTIAMGGIPLTASDSDGFIYDPDGI
                     DTEKLEYILDLKNVKRGRISEYATKYNVPYYEGEKPWMIPCDVALPCAVQNELDKVGA
                     EQLVKNGCKLVAEGANMPTTPEAIEVLHENGIMYAPGKAANAGGVATSGLEMSQNSIR
                     LSWTREEVENKLRDIMRSIHEQCMMHGKREDGTIDYVRGANIAGFVRVADAMLAEGHV
                     "
     gene            1701..2564
                     /locus_tag="PLU11_11685"
     CDS             1701..2564
                     /locus_tag="PLU11_11685"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPN59832.1"
                     /translation="MYQPKAIIEDCGLQVQRFEQVHGGDINSAYCLHTHEGIFFLKVN
                     QAGFYPDMFLKEAAGLSELKKTRTVSIPEVIKTGIAGEQQYLLLEWIERGQPDKQFWE
                     HFGHQLARLHQQPQTYFGWTTDNYIGRLKQSNKRHGNWDEFYTECRIMPLVEILYNKQ
                     LFTRADLNAAGHFCNQLDSLFPVEHPSLLHGDLWSGNYSVSAGGQPVIFDPAIYYGHR
                     EMDLGMTQLFGGFDPRFYKAYQEVYPLEPGWEKRLKLTEAYPLLVHAVLFGGFYIGRA
                     REVLRYFNDWT"
     gene            2749..4140
                     /locus_tag="PLU11_11690"
     CDS             2749..4140
                     /locus_tag="PLU11_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59833.1"
                     /translation="MKPDQIHPKQPGRTQSWLPYFLAGLFLLGILLIFRGFPDEKRFT
                     VVQLKGNGLVTNWSKSFHFIFFPSSNREKGAILTEKNDVLGFNDTYFRYQPTKEDTLR
                     INDEGDSLVFLNSKINSIVIHKNEDLLPWFRQMKPEQLTDLASILIQDPIPESYIPYL
                     KTIASQHPHLSLTFAENDSLNLTTDYIRKAGFFQPTVVFVPVINEEIPALVYWKKAKC
                     LFIVLDDSVILNPLPALPEMKECIVYGDDLKSMPPDFFINNLQLKKLSLLLNKPFYEL
                     LAPLDQLDELVLKNGDSVAGLDKLKNIPERLSVLLISGKCTGIEQLVKAKGLKWLGLP
                     VNTSQKEFNMLTASLKKIQVLEMAGNSNLTDFTNLQEMPDLRALIIIDTVTDKKSIGQ
                     LKKLRYLSLPHDTKEDSAYLKEMAKALPGCIVVANSGACLGSGWLLLLLPLALLSGIL
                     FHKKTNTEHETTG"
     gene            4124..>4465
                     /locus_tag="PLU11_11695"
     CDS             4124..>4465
                     /locus_tag="PLU11_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59834.1"
                     /translation="MKLPAKKIPVNHYGILREVFLVSLCILLFAWLVSWPVPVKWFSF
                     LPLTCAGLIIGPRLFNTALLRSGLFRLGPVKVLYCITGLLMGIAGALYYRGMYSMPLL
                     PAWFHGFVLIAV"
BASE COUNT         1147 a         1105 c          970 g         1243 t
ORIGIN      
        1 agattacctg gcccggatta ccctattgaa aagaatggga caggtacacc cggtggtcac
       61 cgtactcggc gtcatcgtag gccttgggct ctttggcttc atcggactga tctttggtcc
      121 gctactggta aattatatta tcctgctctt ccgcatttac tgcaacgaat tcattcttga
      181 gaagaaataa aaaaaggttg tctcaccaaa gtaagacaac ccaatactta tcaactatct
      241 ttctacggca ggtatacctg ttaacctgtc aatccgttaa cctgtcaacc tccctaaacg
      301 tgtccctccg ccagcatggc atccgctact cttacaaatc cggctatatt cgcgcccctt
      361 acatagtcaa tcgtgccatc ctcccgtttt ccatgcatca tacattgttc atggatactg
      421 cgcatgatat cgcggagttt attctcgact tcttcgcggg tccaggaaag gcggatggag
      481 ttctgagaca tttcaagtcc ggaagtggct acaccaccgg cattggcggc tttacccggc
      541 gcatacatga tgccgttttc atgtaacact tcaatggctt ccggggtggt tggcatattg
      601 gcgccttcgg ccaccagctt acaaccattc ttcaccagtt gctcagcacc aactttatcc
      661 agttcgttct gaacggcaca aggcaacgct acatcacatg ggatcatcca gggcttttca
      721 ccttcataat aaggtacatt gtatttggtg gcgtattcgg atatacggcc gcgttttaca
      781 tttttcagat ccaggatata ttccagtttc tccgtatcaa taccatccgg gtcataaatg
      841 aatccatcac tgtcggaagc ggtcagcgga ataccaccca tggcaatggt tttttcaata
      901 gcatactggg ctacattacc actaccggaa accactaccc ttttattttt cagggtttct
      961 ccttttgtac gcagcatttc atccacaaag aacaataatc catatccggt agcttccgga
     1021 cggatcaggc tgcctccata agtaaagccc ttgccagtca gcacgccggt aaattcattg
     1081 gacagtcttt tgtattggcc aaacaggaac ccgatctcac gaccacccac cccgatatca
     1141 ccggcgggta tatccgtatc attaccaata tggcgataca gttcggtcat gaagctctgg
     1201 cagaaacgca tcacttcatt atcggatttg cccttgggat caaaatccga tccccctttg
     1261 gcacccccca ttggtaaagt ggtcagcgaa tttttgaata cctgttcaaa agccagaaat
     1321 ttcaggatac taagactgac attcgggtga aaacggagcc cgcctttata gggaccgatc
     1381 gcgttgttca tctgaacgcg gtaacccctg ttgacccgga aattaccggc atcatccacc
     1441 cagggtacac ggaaaatgat gatccgttca gcctctgtca aacgttccat aatattcgct
     1501 ttcttgtatt gggggtgttc ctcgatataa ggaataatgg cttgagcaac ttcagaaacg
     1561 gcttgcagga attccggttg atcagggtta cgtttcttca tcaattgcat gaacttatca
     1621 gccattgatt tttttgttgt aatcatacag caaagtttta ggtgtttaca atcgtttaat
     1681 actgaccggt atacggattg gtgtaccaac caaaggcgat cattgaagat tgcggtcttc
     1741 aggttcagcg ctttgaacag gtgcacggcg gggatatcaa cagcgcatac tgcctgcata
     1801 cccacgaagg gatttttttt ctcaaagtaa accaggcggg tttttatccg gatatgtttc
     1861 ttaaagaagc cgccgggctc agcgaattga aaaagaccag gacggtcagt atccctgaag
     1921 tgatcaaaac cgggattgcc ggtgaacagc aatacctgct gctggagtgg atcgaaagag
     1981 gtcaacctga taaacagttc tgggaacatt ttggtcatca gctggcccgg ttacatcaac
     2041 agccacaaac ctatttcgga tggactacgg acaattatat cggccggctg aaacaatcca
     2101 ataaacgaca tggcaactgg gatgaatttt ataccgaatg ccggatcatg ccgcttgttg
     2161 aaatacttta caataaacaa ctctttaccc gtgctgacct caatgccgcc ggacatttct
     2221 gcaaccagct ggactcactt ttcccggtcg aacacccttc gctcctgcat ggtgatctct
     2281 ggagtggcaa ttacagcgtt tctgccggcg gtcagcccgt catctttgac ccggccatat
     2341 attatggtca ccgggaaatg gacctcggca tgacgcagtt atttggcgga tttgaccccc
     2401 ggttttataa agcctatcag gaggtctatc ccctggagcc cggatgggag aagcggttaa
     2461 aactcacaga agcctacccc ctgctggtgc atgccgtgct gtttggcggg ttttatatcg
     2521 gccgtgcccg ggaagtactg cgctatttta acgactggac ctgattcttc ctgtccccgg
     2581 tcatgtacag ggttaacctg catcattgtt ccaccctctt ccacgcagtt acatttcgat
     2641 tgtccagatc ctgtccttaa cccgggagct acgtaccact ctgctttccc gggaaaatga
     2701 tttcccgccg ctcctgtaaa ctgataactc atcttacaaa ctttgtctat gaaaccagat
     2761 caaatccatc cgaaacagcc gggaaggact caaagctggc tgccatactt tctcgcagga
     2821 ctctttttgc tgggtatcct gcttattttc cgcggattcc cggacgaaaa acgttttact
     2881 gttgtgcagc taaaaggaaa tggattggtt accaattggt ctaaatcatt tcattttatt
     2941 ttttttccgt cttccaacag ggaaaaaggg gccatactta cggaaaaaaa tgatgtgctt
     3001 ggatttaatg atacctattt ccgttatcag ccaaccaaag aagatacgct ccggattaac
     3061 gacgagggtg actcccttgt tttcttaaac agtaaaataa attccatagt tatacataaa
     3121 aacgaggatt tgctaccctg gttcaggcaa atgaaaccgg aacagctgac tgatctggcg
     3181 tcaatcttaa tacaggatcc catccctgaa tcctatatcc cctatcttaa aactatcgct
     3241 tcacaacatc cccatttgtc gctcacattt gccgaaaatg attccctcaa cctgacgaca
     3301 gattatatcc gtaaagccgg ttttttccag ccaaccgttg tgtttgttcc ggttatcaat
     3361 gaggaaatcc cggcactggt gtactggaag aaggcaaaat gcctctttat cgtactggac
     3421 gattcagtca tcctgaatcc gctcccggca cttcctgaaa tgaaagagtg tattgtatat
     3481 ggggatgatt taaaatcgat gcccccggac ttttttatca ataaccttca actgaaaaaa
     3541 ctgagcctct tactgaataa gcctttctat gaactcctgg ctccactgga tcagctggat
     3601 gaactggttt taaaaaacgg agatagtgtt gcgggcctgg acaaactaaa aaatataccg
     3661 gaaagattat cggtattgtt gatttccggc aaatgcaccg gcattgaaca actggtaaaa
     3721 gccaaaggat tgaaatggct gggattgccg gttaatacat cacaaaaaga gttcaatatg
     3781 cttaccgcca gcctgaaaaa aatacaggta ttggaaatgg ctggtaattc caacctgacc
     3841 gattttacaa acctgcagga aatgccggat ctccgtgcac tgataattat tgatacggtg
     3901 accgataaaa aaagcatcgg ccagttgaaa aaactgcgtt atctctcctt accacatgat
     3961 acgaaagaag acagtgctta tctgaaggaa atggccaaag ccttgcccgg ttgtattgta
     4021 gttgccaaca gcggggcctg tctgggttcc ggctggctgc tattattact tccattggcc
     4081 ctgctgtctg gaatactgtt ccataaaaaa acaaatactg aacatgaaac taccggctaa
     4141 aaaaatacct gtcaatcatt acgggatact ccgggaagtg ttcctggtca gtttgtgtat
     4201 tttattattt gcctggctgg tcagttggcc tgtgccggtg aagtggtttt ctttccttcc
     4261 acttacctgt gccggtctga ttattggccc ccggcttttc aataccgcct tgctcaggtc
     4321 cggtttgttc cgcctcggtc cggtcaaggt tttatactgt attacaggcc tgttgatggg
     4381 aattgccggc gccttatact accggggtat gtattcgatg ccgcttttac cggcctggtt
     4441 tcatgggttt gttttgatag cggtt
//
LOCUS       DAOPMW010000271         4464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22851_length_4464_cov_8.288047, whole genome shotgun sequence.
ACCESSION   DAOPMW010000271 DAOPMW010000000
VERSION     DAOPMW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22851_length_4464_cov_8.288047"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLU11_11700"
     CDS             <1..1016
                     /locus_tag="PLU11_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HPN59835.1"
                     /translation="FRDPKVSWNTAANKWIMTLAVADRIHFYSSPDLKIWTKESEFGE
                     KAGAHGGVWECPDLFPMKHDGKEIWVLLVSLNPGGPNKGSATQYFLGDFDGKVFHPYS
                     TETKWIDYGPDNYAGVTWSNTRDKTFFMGWMSNWSYAQVVPTTTWRSAMTLPRELRLE
                     KAGNELRIASWPVKSLLPMLSGTVISKGMGKSPYDVFRMSNKSGIPCQINLSATKHAF
                     SLRFSNEAGEELLIGYDDTLNQYYIDRSKSGNTAFHADFPLRTVAPRISLTDTIKLSV
                     VVDQSSVEVFADKGLTAMTALYFPSGSFTKAELISAMPFKFDAVELTNLGMQKTNVTK
                     TSQ"
     gene            complement(991..2409)
                     /locus_tag="PLU11_11705"
     CDS             complement(991..2409)
                     /locus_tag="PLU11_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPN59836.1"
                     /translation="MKNILLCLLLLTLQQAVVLAQAKRPNIIFIFADDLGYGEIGCYG
                     QQKIETPQIDKMATAGIRFTRFYSGSTVCAPARTSLLTGMHTGHTLIRGNLPFAPEGQ
                     IPLPENAKTFANYLQENGYATAAFGKWGLGFNQNSGDPNKHGFDRFYGYNCQSLAHDY
                     YPPYLWDNHQKVELPNNGADSIYSAALIHQQAINYIKAAGTQPFFMYLPYTLPHGDVI
                     GPHDEWYKHYAQKFNEAPLTGNKLKTRAHNAVPEPLPHAQFAAMISRLDQYVGEIIKA
                     IADKGLAENTLIIFTSDNGPHRENGGDPDFFNSSGAFRGIKRDLYEGGIRVPYVAYWP
                     GKIKPAVNEDPAALWDMYPTILELAGVKKKVKTDGLSLVPVFFQKGKRKTHEYFYWEF
                     HENDGRQAISWKNWKAIRLNVSTDSPSPVELYDLSKDPDEKHNLADQYPSIVNKLSGW
                     MMKAHQYNPKWPLLFTEKSLSH"
     gene            complement(2413..3540)
                     /locus_tag="PLU11_11710"
     CDS             complement(2413..3540)
                     /locus_tag="PLU11_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN59837.1"
                     /translation="MKRILLLTGFLSLMLSSFSQSLPKRKKILKTMQLTNQYFMNKWP
                     DAGKSIFTNIERPSNIWTRAVYYEGLMSLYGIDKKKTYYDYMLQWGEKHKWGLRGGIK
                     TRNADNQCCGQTYIDMYILDGQQRPERVKDIKACIDTMLSTPKVDDWNWIDALQMGMP
                     VFVKLGNLYNDTRYFDRMYEMYAFTKYKHGGKGLYNTAEKLWWRDKDFVPPYKEPNGD
                     DCYWSRGNGWVVAALVKTLEALPKSDSHYNEYLQDYKDMCAALLPLQRTDGYWNVSLN
                     DPAHFGGKEVSGTSLFIYGFAWGINHGLLDKKTYKPAIVKAWKAMAKEAVHPDGKLGF
                     VQGTGKEPKDGQPVGYDKTPDFEDYGLGCFLLAGTEIYKMK"
     gene            3687..4001
                     /gene="rhaM"
                     /locus_tag="PLU11_11715"
     CDS             3687..4001
                     /gene="rhaM"
                     /locus_tag="PLU11_11715"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPN59838.1"
                     /translation="MQRLAFKMKLFKGQEAEYRKRHDEIWPELSQLLKDNGISDYSIF
                     LEEETGFLFGVLKVEDPANMEVLPAQAIMKKWWAYMKDIMESNDDNSPVSVPLREVFY
                     MD"
BASE COUNT         1179 a          987 c         1030 g         1268 t
ORIGIN      
        1 atttccgtga tcccaaagtt tcctggaata cagccgccaa taaatggatc atgacgctgg
       61 ccgtggccga ccggattcat ttctattctt ctccggattt aaaaatctgg accaaagaaa
      121 gtgagtttgg ggagaaagcc ggcgcacatg gtggcgtatg ggaatgtccg gatctttttc
      181 cgatgaaaca cgatgggaaa gaaatctggg tattactggt cagtctgaac ccgggtggtc
      241 cgaataaagg ctcggccact caatacttcc tgggcgattt tgacgggaag gtttttcatc
      301 catacagcac cgaaacaaaa tggatcgatt acgggccgga taattatgcc ggggtgacct
      361 ggagtaatac ccgtgacaaa accttcttta tggggtggat gagtaactgg tcttatgcgc
      421 aggtggtgcc cactactacc tggcggagtg cgatgaccct gccgcgtgaa ctacggttgg
      481 aaaaagccgg aaatgaatta cgtattgctt cctggcccgt aaaatcactc cttcctatgt
      541 taagtggtac ggttatttca aaaggcatgg gaaaaagccc gtatgatgtt tttcgcatgt
      601 caaacaaatc cggcattcct tgccagatca atctctcggc gactaaacat gctttctctt
      661 tacgcttttc aaatgaagcc ggggaagaac tgctgatcgg ttatgacgat acattgaatc
      721 aatattatat cgaccgcagc aagtcgggca atacagcctt tcatgccgac ttcccattaa
      781 ggactgtggc gccacgtatc agtttaaccg atactattaa gctttctgtg gtagtggatc
      841 agtcctccgt tgaagtattc gccgataagg gtcttacggc tatgacggcc ttgtactttc
      901 cttcgggttc atttacaaag gctgaactga tttccgcaat gccttttaag ttcgatgcgg
      961 ttgaactgac taacctcggt atgcaaaaaa ctaatgtgac aaagacttct cagtaaagag
     1021 tagcggccac ttcggattat actgatgcgc cttcatcatc cagccggaaa gtttatttac
     1081 aatggaaggg tattgatccg ccagattatg cttctcatcc gggtctttgg ataaatcata
     1141 gagttctaca ggggatggac tgtccgtaga aacattcaac cggatcgctt tccagttctt
     1201 ccagctgatc gcctgccgac catcattctc atgaaactcc caataaaagt attcatgtgt
     1261 ttttcgtttg cctttttgaa agaaaacagg aaccaatgac agaccatctg ttttgacttt
     1321 cttctttacg cccgccagtt caagtatggt cggatacata tcccataaag cagcgggatc
     1381 ttcatttaca gccggtttga ttttgccggg ccagtaggcc acataaggca cgcggatccc
     1441 gccttcatac agatcacgtt tgatgcctcg gaatgcgcca ctgctattga agaaatccgg
     1501 atcgccgccg ttctcccgat gaggaccgtt atcactggta aaaatgatca gggtgttttc
     1561 ggccagtcct ttatcggcaa tagcctttat gatttcgcct acatactgat ccagtcttga
     1621 gatcatggcc gcaaactgtg catggggcag gggctcgggt actgcattat gggcacgggt
     1681 cttcagctta tttccggtca gcggtgcttc attaaacttt tgggcataat gtttatacca
     1741 ttcatcatgc ggaccgatca catcaccatg tggtaaggta taaggcaggt acatgaagaa
     1801 gggttgtgtt cctgctgcct tgatataatt aatggcctgc tggtgaatca gtgcggcaga
     1861 gtaaatggaa tcagccccgt tattgggtaa ttccactttc tgatgattat cccagagata
     1921 aggcgggtaa taatcatgcg ccaggctctg gcaattgtat ccgtaaaaac gatcgaagcc
     1981 atgtttgttg ggatcgccgc tgttctggtt aaaacccagt ccccatttcc caaaagccgc
     2041 agtagcatac ccgttttcct gcaggtagtt tgcaaaggtt tttgcatttt caggtaatgg
     2101 aatttgtcct tccggtgcaa aaggaagatt gccccggatc agcgtatgtc ccgtatgcat
     2161 accagtgagc agggaagtgc gggccggtgc acagacagta gaaccggaat agaaacgggt
     2221 aaagcggata cccgctgttg ccattttatc aatctgaggg gtttcgatct tttgttgtcc
     2281 gtaacaacca atctccccat aacccaggtc atcggcaaag atgaaaataa tattgggtct
     2341 tttggcctgg gccagtacaa cggcttgttg taacgtaagg agcaataaac aaagaagtat
     2401 gtttttcatg ccttacttca ttttataaat ttccgtcccg gccagcagga aacaaccgag
     2461 tccgtagtct tcaaagtcgg gggtcttgtc atagcccact ggttgaccat ccttgggttc
     2521 tttgccggtg ccctgcacaa agccgagttt accatcggga tgtaccgctt ctttcgccat
     2581 cgctttccag gctttcacta tcgcgggctt ataggttttt ttatccagta aaccgtgatt
     2641 gatgccccag gcaaaaccat agataaaaag ggaagtaccg gatacttctt taccaccaaa
     2701 atgtgctggg tcgttcagac ttacattcca gtaaccatcc gtacgttgca ggggcaggag
     2761 ggcggcacac atgtctttat aatcctgcag gtattcattg taatgcgaat cggacttggg
     2821 taaagcctcc agggttttca ccagggcggc cactacccag ccattaccgc ggctccagta
     2881 acaatcatcg ccattgggtt ctttatatgg cggtacaaag tctttatctc tccaccagag
     2941 tttttcagcg gtattgtaca aacccttgcc accatgtttg tatttggtaa aagcatacat
     3001 ctcgtacatc cggtcaaaat agcgggtgtc attatagaga ttgcccagtt tgacaaatac
     3061 cggcataccc atctgcaagg catcgatcca gttccagtca tccaccttgg gggtggagag
     3121 catggtgtca atgcaggctt tgatatcttt cacccgttca ggacgttgct gtccatcgag
     3181 gatatacata tctatataag tctgaccaca acattgatta tccgcattac gggttttgat
     3241 accaccgcgc agtccccatt tgtgtttttc gccccactgg agcatatagt cataataggt
     3301 tttctttttg tcaataccat aaagcgacat caatccttcg taatagaccg cccgggtcca
     3361 gatattactg gggcgttcga tattggtaaa aatggattta ccggcatcag gccatttgtt
     3421 catgaaatac tgattggtca gctgcatcgt cttcaggatc tttttacgct tgggtaaaga
     3481 ctggctgaaa gatgagagca tcagcgacaa aaaaccggtg agcaagagga ttcttttcat
     3541 atacaatcgt tatgctttta attcagtgtg tcgtaaaatt cattcaaaaa ccggagattt
     3601 ggtaaattca ttcctttaaa aaaaacagac agtacgtgac agtggacagg gaaggttcag
     3661 atactttaaa tctataaata aaaaatatgc aacgattggc tttcaaaatg aaattattca
     3721 agggacagga agcggagtat cgcaagcggc atgatgaaat ctggcctgag ttatcgcaac
     3781 tgctgaaaga taacgggatc agcgactatt cgatcttcct ggaagaagaa accggttttc
     3841 tgtttggggt gttgaaagtg gaggatccgg caaatatgga agtattacct gcccaggcca
     3901 ttatgaaaaa atggtgggcc tatatgaagg atattatgga gagtaatgat gacaattccc
     3961 ctgtttccgt gccattaaga gaggtttttt atatggactg acacccgttg agaaggcaga
     4021 ttcatctaaa gaaagatact aaaagctgat accggtgttc cgggaacgat tgcgtaaaaa
     4081 aatacgagtt cctttgactt gcttttttct gaatttttag gtagaaagta cccctataac
     4141 gttacataaa acagtaataa tcaatttcaa atgcgcttat tgctcctttt tttatcaggt
     4201 agatctgtca aactttaaag gcagttcaca tttccatcct ctcttaagtc tgtttcgcag
     4261 gatgcatcag gatggtttct tctcaatcaa tcagttattt agtacagttt ttcgactgta
     4321 tctggccggg aaaacagggt tttaagccag atgattgcta attgcttata tgaacaacat
     4381 gttatttcag aaccaagctg catttatgtg cgctcaatat atttctgcat tgaccgggaa
     4441 ggctcctgcc ttacccggtt tttt
//
LOCUS       DAOPMW010000272         4460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_22876_length_4460_cov_8.596141, whole genome shotgun sequence.
ACCESSION   DAOPMW010000272 DAOPMW010000000
VERSION     DAOPMW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_4460_cov_8.596141"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLU11_11720"
     CDS             complement(<1..712)
                     /locus_tag="PLU11_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59839.1"
                     /translation="MIKKRLITICSVLLIAASCFSQGKTALSLTKGKYGDGPDSIAPS
                     GIIKNLNDEQLLELVQKQTFRFFWHGAHPYSGLALERSNTVLAEHYWDYINEAWDEPN
                     FSKTVFGPDAGAIGGTGFGIMSTVVAVDRKWIGRDTAVRRLIQITDFLINADCYHGIY
                     PHFMNGRTGKTIKFDRLDDGADIVETSYLLMGLLCAREYFTGNTPREVYLRKRINQIW
                     GAANWNWHTNGQPKLYWHW"
     gene            complement(722..2737)
                     /locus_tag="PLU11_11725"
     CDS             complement(722..2737)
                     /locus_tag="PLU11_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN59840.1"
                     /translation="MYTARKTILIFLHLLFVGTAARGQQGFGAPVYWQHFGISPKGEA
                     VIGPALPQGRTSLQRSTTLCPPPGYYSIIKRVPAGSCFNDEWIPLSNDQNSTYDMTHA
                     EGNLMLVNNTVATGSRLLYMDTVQQSLCPGIDYFFSAAIINVDRPVTCPGSHFFPRLA
                     FSLETEAGQILVTDTIRNGISFANPFMGYKFGEFGIWLNMPAITGRLVLRIDLLTTYY
                     DCAEDFAIDDIRLRPKGAAMNIQFPGEPNNTVKAACYQQHPTITFSGTMEPYYNNPAL
                     QWQISMDSGATWTDIPGATSPLLTQTYTLPDTFYYRLSGADLSMITNPACRVVSNKLR
                     VEIQGPPAGYTISSNSPVCSGLPLLFKADGAASYEWNGPNGFYDNVPTPSIYYSTLAD
                     SGVYYIAVSSWGGCITMDSIRVKIIGTDVRAWPDTAICIGNTVSLHASPGISYAWTGD
                     GVTGSALQHPLIKPAKNGRYTVKVTDTSGCSDTASVQVIIRNNVPVKAIMDFSPYLCR
                     VYDSLLFKERSQGHLKTWHWDFGNGISSTEQKPATQYYNIAGNQFMYMSRLAVADTAG
                     CTDTAYQLIQVEDNCYIAVPTGFTPNNDGRNDYLYPLNAFKVTDLHFRVFDRRGQVVF
                     ESRDWSRKWDGRVGGVEQTTGVYVWTLEYTEVNGKKVFLKGTTTLIR"
     gene            complement(2758..4104)
                     /locus_tag="PLU11_11730"
     CDS             complement(2758..4104)
                     /locus_tag="PLU11_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPN59841.1"
                     /translation="MRIKRQLLTGVMALLLLPAQGQKTDPLFNPAGRPKNLTDSALLD
                     LVQKQTFRYFWDFAHPVSGMARERSNESYGYGEETVTTGGTGFGVMSVIVATERKWIS
                     RDTAAKFLLKLINFLLKADSYHGAFPHWLNGATGKTIPFSRKDDGADLVETSYLFQGL
                     LCARQYFTGTNATETELRNRISWMWPDIEWNWFTNGGQEVLYWHWSPNNGWAMNFPVR
                     GFNECLIMYVLAASSPNEKYSVSPAVYHRGWAESNFFKNGKSFYDIKLPLGFDYGGPL
                     FFSQYSFLGLDPRGLKDRYADYWEQNLNHTLINREHCVRNPNKFKGYGENCWGLTASD
                     TYNGYAAHSPTEDLGTISPTAALSAFPYTPVYSMQALRHFYTDLGDKIWSEYGFVDAF
                     NESKNWVASSHLAIDQGPIIVMIENYRSGLLWNLFMSCPEIQTGIKRLGFTSPASH"
     gene            complement(4133..>4460)
                     /locus_tag="PLU11_11735"
     CDS             complement(4133..>4460)
                     /locus_tag="PLU11_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPN59842.1"
                     /translation="TASSPTNDRGVIAPTAALASFPYTPDQSMAALKFFYYVLGDKIW
                     KEYGFVDAFSLRDTWFATSFLAIDQGPVIIMLENHRSGLLWNLFTSCPEVKTGMLSLG
                     FSAPYL"
BASE COUNT         1157 a         1105 c         1045 g         1153 t
ORIGIN      
        1 tccagtgcca gtagagtttg ggttgtccgt tggtatgcca gttccagttg gcggcacccc
       61 agatctggtt gatccgttta cggaggtata cttcccgggg ggtgttgccg gtaaagtact
      121 cccgggcaca gagtaagccc atcagcaggt aggaggtctc tacaatatca gcgccatcat
      181 cgagacggtc aaacttaatc gtcttgccgg ttctgccgtt catgaaatgc ggatagatgc
      241 catgatagca atcggcatta atcaggaaat cggtaatctg aatcaggcgt ctaactgccg
      301 tatccctgcc gatccacttg cggtccacag ccaccacggt actcatgata ccaaaaccgg
      361 taccacctat ggccccggca tccggtccga aaacagtctt actgaaattg ggttcatccc
      421 atgcttcatt gatataatcc cagtaatgtt ccgccaatac agtattgctc ctttccaagg
      481 ccagtccgct gtacggatgg gctccatgcc agaaaaaacg gaaggtttgc ttctgtacca
      541 gctccagcaa ctgttcatca ttcagatttt taataatgcc ggagggtgca atactgtccg
      601 gaccatcccc gtatttcccc ttggtgagtg acagggccgt cttcccctgt gaaaagcagg
      661 aggctgcgat cagtaataca ctgcaaatgg taatcaatcg ttttttaatc ataacggtgt
      721 tttatcgtat caatgtagtg gttcctttta aaaaaacttt tttcccattc acttcagtat
      781 actccagtgt ccatacatag accccggtcg tctgctccac cccgcctact cgaccgtccc
      841 atttccggct ccagtcacgg ctttcaaata ccacttggcc tctgcggtca aatacacgga
      901 agtgcaggtc ggttacttta aatgcattca gcggatagag ataatcattc cgtccgtcat
      961 tattgggagt gaagcccgtg ggtaccgcga tatagcaatt gtcttctacc tgtatcagct
     1021 gatacgctgt atcggtacag ccggctgtat ccgcaacagc cagccgggac atatacatga
     1081 attgattacc cgctatgttg taatactggg tagcgggttt ttgttccgtt gaactgatcc
     1141 catttccgaa atcccagtgc caggttttca aatggccctg gctcctttct ttgaacagca
     1201 atgaatcata caccctgcag agataaggcg aaaaatccat gatggctttt accggcacat
     1261 tattccggat gatcacctgc accgaagcgg tatctgaaca accggatgta tcagtgactt
     1321 tcacggtata ccgtccgttc ttcgccggtt ttataagtgg gtgttgcagg gcgctgccgg
     1381 taactccgtc accggtccat gcataactga taccgggact ggcatgcagg gaaactgtgt
     1441 ttcctataca aattgccgta tccggccagg cgcgaacatc tgtaccgatg attttcaccc
     1501 ggatagaatc catagttatg caaccacccc agctggatac agctatataa tacacaccac
     1561 tgtctgcaag tgtgctgtaa taaattgaag gggtgggtac attatcataa aaaccattgg
     1621 ggccgttcca ttcataactg gctgctccat ctgctttaaa taacaagggc agtcccgagc
     1681 aaaccggcga attactgctg atggtataac cggcaggtgg tccctgaatt tccacccgga
     1741 gtttattgga taccacccgg caggccggat tggtaatcat actcagatct gcaccactca
     1801 gcctgtaata aaaagtatcc ggcagtgtat aggtttgtgt gagtaacggg ctggtggcac
     1861 cgggaatatc ggtccaggtg gcgccggaat ccatacttat ttgccactgc aaggcaggat
     1921 tattgtaata tggctccatg gtaccgctga atgtaattgt gggatgctgc tgatagcagg
     1981 ccgctttcac tgtattattc ggttcgccgg gaaactgaat attcatagcg gccccttttg
     2041 gacgaagccg gatatcatca atagcaaaat cctccgcaca atcataatat gtcgtcaaca
     2101 ggtcaatccg taagaccaac ctgcctgtaa tggccggcat attgagccag ataccgaatt
     2161 cgccaaactt atagcccata aacggattgg caaaagagat cccgtttcgt atagtgtctg
     2221 tcaccaggat ctgaccggct tcggtttcca gtgaaaatgc aagccgtgga aagaaatggg
     2281 agcccggaca ggtaacgggt ctgtctacat ttataatagc cgctgaaaaa aaataatcaa
     2341 ttcccggaca aagggattgc tgtaccgtat ccatatacaa caggcgcgaa ccggttgcca
     2401 cggtattgtt caccagcatc aggttgcctt cagcatgggt catatcatag gtgctgttct
     2461 gatcatttga tagcggaatc cattcatcat tgaagcaact gcctgcaggt acccgtttga
     2521 taatagaata atatccggga ggcgggcaaa gggtggtact gcgctgaagg gaggtgcgac
     2581 cctggggcaa ggccggcccg ataactgcct ctcctttggg agaaatcccg aaatgctgcc
     2641 agtataccgg tgcaccaaaa ccctgctgac ctcttgcagc agtgccaaca aacagcaggt
     2701 gtaaaaatat taatatcgtt ttcctggccg tatacattca ataccccggg gcttgtttca
     2761 atgtgaggcg ggactggtaa acccgagtcg ctttattccg gtttgaattt ccgggcagct
     2821 cataaagaga ttccacaaca gtccgctccg gtaattttct atcattacaa taatggggcc
     2881 ctggtcaatc gccaggtgag aagaggctac ccagtttttt gattcgttga aagcatccac
     2941 aaaaccatac tcactccaga ttttatcacc gagatcagta taaaaatgac gtaatgcctg
     3001 catcgaatat acgggtgtgt aagggaatgc gctcagcgcg gctgttgggg aaatggtgcc
     3061 aaggtcctcg gtgggggaat gcgcggcata tccattatag gtatcactgg ccgttaagcc
     3121 ccagcagttt tcaccatagc ctttaaattt atttggatta cgtacacaat gttcccggtt
     3181 gatcagggta tggttcaggt tttgttccca gtaatcagcg taccggtctt ttaatcctcg
     3241 cggatcaagg cccaggaaag aatactgtga gaaaaacaat gggcccccat aatcaaatcc
     3301 aagcgggagt ttgatatcat aaaatgattt cccgtttttg aagaaattac tctccgccca
     3361 gccccggtga tacacggccg gagatacact gtatttttca ttgggagatg aagccgcgag
     3421 aacatacatg atcaggcatt cgttgaatcc ccgtacagga aaattcatgg cccatccgtt
     3481 attcggactc cagtgccaat acaatacttc ctgtccgcca ttggtaaacc agttccattc
     3541 aatatccggc cacatccagg atatccggtt acgcaattct gtttcagtgg cattggtgcc
     3601 agtgaaatac tgacgggcac ataacaatcc ctgaaataag taagaagttt ccaccaggtc
     3661 agccccgtca tcttttcggc tgaacgggat tgttttgccg gtggcgccat ttaaccaatg
     3721 tggaaaggct ccgtgatagc tatctgcttt cagtaaaaaa ttaattagtt taaggaggaa
     3781 ttttgccgct gtatcgcggc tgatccattt tctttcagtg gctacgatta cgctcataac
     3841 cccaaaacct gttccgccgg tggttaccgt ttcctcacca tagccataac tttcattact
     3901 tctttccctg gccatcccgg agaccggatg agcaaaatcc cagaaataac gaaatgtctg
     3961 tttttggacc aggtcaagca gcgcactgtc cgttaagttt ttgggtcggc ctgccggatt
     4021 gaatagcggg tcagtctttt gtccctgagc cggcaaaagt aacaaggcca taaccccggt
     4081 aagaagttgt ctctttattc gcataagatg ttcttgattc aggaatagat cttcacaggt
     4141 atggcgcact aaaaccaagg gataacatcc cggtttttac ttccggacaa ctggtaaaca
     4201 ggttccacaa caaaccgctg cggtgatttt caagcatgat gataacaggc ccctggtcaa
     4261 ttgccagaaa agaagtggca aaccaggtat cccttaatga aaaggcatcc acgaaaccat
     4321 actctttcca tattttatca ccgagtacat aataaaagaa tttcaaagcg gccatagact
     4381 ggtcaggtgt atacggaaaa gacgccaggg cggctgtagg cgcaatcacc cccctgtcgt
     4441 tggtgggtga actggccgta
//
LOCUS       DAOPMW010000273         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23044_length_4440_cov_6.315165, whole genome shotgun sequence.
ACCESSION   DAOPMW010000273 DAOPMW010000000
VERSION     DAOPMW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23044_length_4440_cov_6.315165"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLU11_11740"
     CDS             complement(<1..913)
                     /locus_tag="PLU11_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN59843.1"
                     /translation="MKNILFFIVLLTTSLLSLAQQKVIPLYDGVAPGTETWNWSEAVN
                     DNNSWNTKVVYNVTQPSLTVFQPAAGKANGTAVVICPGGGFYAHSINSEGFDVAEWLV
                     KKGVTCFVLKYRLAHTTGTDPTEELNRVMGTKEFETVTAKIIPMAVADGKAAIAWVRK
                     HAAEYNLIRNRIGIMGFSAGGTVASSALYNYTAENKPDFAAPVYPFFPKEMQGTIGTD
                     LPPVFIAAATDDQLGLAPHSADLYNSLMASRKSVELHMYARGGHGFGMRKQNLPSDQW
                     IDRFGEWLDQQGMMKRPVFNPDFALFEQ"
     gene            complement(993..3311)
                     /locus_tag="PLU11_11745"
     CDS             complement(993..3311)
                     /locus_tag="PLU11_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPN59844.1"
                     /translation="MKYLLAVAAALVITSAKAQTPSAKTLLSKMTLEEKVNLVVGMGM
                     NIPGLNTGAPVVGQSMDKVAGAAGTTFAMPQLGIPNTVLADGPAGLRIDPTRKEDRRT
                     YYATAWPVATLLASTWDTPLVEKVGKAMGNEVKEYGVDVLLAPALNIHRNSLGGRNFE
                     YYSEDPLVSGKMAAAMVNGVESNGVGTSIKHFAANNQETNRNTVNTILSERALREIYL
                     RGFQITVKESQPWTVMSSYNLINGVYASEDYNLLTTILRKEWGFKGLVMTDWFGGKDA
                     VAQMKAGNDLLMPGTAQQKKAILDAVNNGTLDIKILDQNAERIVNYILGSPAYKKFDF
                     SNKPDLTAHAHLVRSAAAEGMILLKNDGQALPLSTKNKLAAFGNTSYSFIAGGTGSGD
                     VNEAYTISLVQGLQNSGYTLESDLQKAYSSHLDDYNSKHPKKDFFTEIMNPTPSAPEF
                     AGYSSMLSKEADNTDAALITIGRNAGEFRDRKKENDFYLSAAETSLIKEVAAAYHAKG
                     KKVIVIINAGGVIDIASWSNEVDAILLTWQGGQEAGNAVADILSGKVSPSGKLATTIP
                     IKYEDEYSSKNFPGVEFPEKATTGMLGMKAVPAEVRYEEGIYVGYRYFNTFNVKPAFP
                     FGYGLSYSSFSYSNLKMSSTQMNGKITASITIQNTGKTAGKEVVQVYITAPGKTMDKP
                     AIELKAFAKTRLLAPGEKQTLQFVLTATDLASFDPARSVWVAEPGEYQVKVGASSQNI
                     LQTARFTLAKEIITEKCNKVMLPEMPVKELTH"
     gene            complement(3379..3942)
                     /locus_tag="PLU11_11750"
     CDS             complement(3379..3942)
                     /locus_tag="PLU11_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPN59845.1"
                     /translation="MDALKKAVFGISPLSPIEEKAFMGAWKCWDLEKDHVLLREKMVS
                     DYIYFVDKGIARIFYHKNDKEITEWIAMDQQFFLSITSFFQRSPSHLSIHTLEPARIY
                     GIHHTDFMSLADRYHEVERLLRKMVTGSLILSQIRMDSIQFETAYQRYERLLRTYPEI
                     IQRVPLMYIASFLGVTLETLSRIRSSR"
     gene            3978..>4440
                     /locus_tag="PLU11_11755"
     CDS             3978..>4440
                     /locus_tag="PLU11_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPN59846.1"
                     /translation="MIQLPPYLQKGDTIGLVCPAGYMPFEKVKTCVQILQEWGYNVKV
                     GNTVGGGSMTYFSGTDDERLRDFQQMLDDNEVKAVLCARGGYGTGRIIDRIDFKKFQK
                     QPKWIVGYSDITVLHSHLFSNYYIASLHAPMAGAFNEEGFKNEYVLSLKNNL"
BASE COUNT         1097 a         1072 c         1043 g         1228 t
ORIGIN      
        1 gttgctcaaa taaggcaaaa tccggattaa agaccggtct tttcatcatt ccctgctgat
       61 ccagccattc tccaaagcga tcgatccact gatcggaagg caggttttgt ttgcgcatcc
      121 cgaatccatg tccgcctctg gcatacatgt gcagttccac agatttacgg gaggccatca
      181 aactattata cagatcagca ctatgcgggg ccaggcctaa ctggtcatca gtcgctgctg
      241 caataaatac aggtgggaga tctgttccga tagtgccctg catttctttc gggaagaacg
      301 gatagaccgg agctgcaaaa tcaggtttgt tttccgctgt ataattatac aaggcagaac
      361 tggccacagt tcccccggca gaaaatccca tgatgccgat ccggttgcgg atcaggttat
      421 attcagcggc atgttttcta acccaggcaa tcgctgcttt cccgtcggca acggccatgg
      481 ggattatttt agcagtgacc gtttcaaatt ctttggtccc catcacccgg ttgagttctt
      541 cagttggatc tgtgccggtg gtatgtgcca gccggtattt caacacaaaa caagtcacgc
      601 ctttttttac cagccattcg gccacatcaa atccttcgct gttgatggag tgggcataaa
      661 atccgccacc cggacaaata actacagctg taccatttgc ctttcccgct gcgggttgga
      721 aaacggtcag actgggctgg gtaacattat agactacttt ggtattccag ctgttattgt
      781 cattcacggc ttccgaccag ttccaggttt cagttcccgg ggcaacacca tcatataacg
      841 gaattacctt ttgttgtgcc aggctcaata gggaagtagt cagcaggact atgaagaata
      901 gtatgttttt catatgcgca gtttaaggac tataaaagta aatgaaaaac ccttcgccgg
      961 agggaagggt ttttatgatt cctgtcaggg ccttaatgag tcagttcttt aactggcatt
     1021 tccggcagca ttaccttatt acatttttct gtaatgattt ctttggccag tgtaaaccgg
     1081 gcagtttgta aaatattctg cgaggaagca cccaccttaa cctggtattc gccaggttcg
     1141 gccacccata cactgcgtgc cggatcgaag gaagcaaggt cggttgctgt caggacaaat
     1201 tgcagggtct gcttttcgcc cggagccagg agcctggtct tggcaaaagc tttcagttca
     1261 atggcgggtt tatccatggt cttgccggga gcggtaatat agacttgtac cacttccttg
     1321 ccagcagttt tgcccgtatt ctgaatggta atactggccg tgatcttacc gttcatttgg
     1381 gtactgctca ttttaagatt actataggag aaggaagaat aactgagacc ataaccaaac
     1441 gggaaagcgg gctttacatt aaaagtattg aagtaacggt agccgacata gatcccttct
     1501 tcataccgga cttcagccgg aacggctttc atccccagca taccggtagt ggctttttca
     1561 ggaaactcca caccggggaa atttttggag gagtactcat cttcatattt tattggaata
     1621 gtagtggcca gtttgccaga agggctcact ttgccactca gaatatccgc tacggcatta
     1681 cctgcctcct gtccgccctg ccaggtcagc aggatggcat ccacctcatt actccaggaa
     1741 gcgatatcga ttacaccacc ggcattgatg atcacgatga cttttttacc ttttgcatga
     1801 taagcggccg ccacttcttt gatcaggctg gtttcggcag cagataaata aaaatcattt
     1861 tcttttttgc ggtccctgaa ctccccggca tttcgtccga tcgtgatcag ggcggcatcg
     1921 gtgttatccg cttctttgga aagcatggaa ctgtaacccg caaattccgg tgctgaagga
     1981 gtagggttca tgatctcagt aaagaaatcc tttttggggt gtttgctgtt atagtcatcc
     2041 aggtgactgc tgtaagcttt ctgtaaatcg ctttccagtg tataaccact gttttgtaat
     2101 ccctgaacca gggaaatcgt atacgcttca ttcacatccc cgcttcctgt acccccggca
     2161 ataaaactat aggatgtatt tccaaaggca gccagtttat ttttagtact caatggcaag
     2221 gcctgcccgt catttttcag caggatcata ccttctgccg cagcgctgcg caccaggtgg
     2281 gcgtgtgcgg taagatcagg cttattactg aaatcgaatt ttttgtatgc gggtgatccc
     2341 aggatataat tcacaatcct ttctgcattc tgatcgagaa ttttaatatc cagtgtacca
     2401 ttattgaccg catccaggat ggctttcttt tgctgggccg taccgggcat cagcaggtca
     2461 ttaccggctt tcatctgggc tacggcatct ttacccccaa accaatcagt catgaccagt
     2521 cctttaaaac cccattcctt ccggaggatg gtggtcagca ggttataatc ttcagaagcg
     2581 tacaccccgt taatcaggtt atacgaactc atgacggtcc agggttgtga ttctttcacg
     2641 gtgatctgga atccgcgtag atagatttca cgaagggccc tttcgctgag aatggtattg
     2701 actgtattac ggttggtctc ctggttattc gcggcaaagt gtttgatgga tgtgcccacc
     2761 ccattacttt ccactccatt caccatggcg gcagccattt ttccggagac cagcgggtct
     2821 tcagaataat attcaaaatt tcttccgccc agtgagttgc ggtggatatt gagcgccggc
     2881 gccagcaata catccacgcc gtattccttt acttcattcc ccatggcctt ccccactttt
     2941 tccaccaatg gagtatccca ggtagaagcc agtaaagtgg ctaccggcca ggccgtggca
     3001 taatacgtgc gccggtcttc ttttcgcgtc ggatcaatac gcaatccggc cggaccatcg
     3061 gccagtacgg tattgggtat cccgagttga ggcatggcaa aggtggtccc tgctgcacct
     3121 gccactttat ccatggactg tcccaccacc ggggcaccgg tattgaggcc gggaatgttc
     3181 atgcccatgc ctacgacgag atttactttt tcttccaggg tcattttcga cagcagtgtt
     3241 tttgctgaag gagtttgtgc ctttgcagag gtgattacaa gtgctgccgc aacagccaga
     3301 aggtatttca tatatcaatc gtttaatgtt taaaggtccg gcacttaaca ttaacctgat
     3361 ttgatatata tcaaaatatc aacgggagga acggatacgg cttagtgttt cgagcgtaac
     3421 tccaaggaaa gaagcaatat acatcagggg cacccgttgg ataatctcgg gataagttct
     3481 taataaacgt tcataccgct ggtacgccgt ttcaaactgg atggagtcca ttcttatctg
     3541 ggagaggatc agggagccgg tgaccatttt gcgcaggagc cgttctactt catgatagcg
     3601 gtcggccagt gacatgaaat cggtatgatg aatcccgtat atccgggcag gttcaagggt
     3661 gtgaatgctg aggtgactgg gactccgctg aaaaaaactc gtgatcgaca aaaaaaactg
     3721 ttgatccatc gcgatccact ccgtgatttc cttgtcgttt ttgtgataga aaatccgggc
     3781 gatgcctttg tctacaaaat atatataatc actcaccatt ttctcccgga gcaatacatg
     3841 atccttttcc aggtcccagc atttccaggc acccataaaa gccttttctt ctatcggaga
     3901 aaggggcgaa ataccgaata ctgctttttt caatgcatcc atatccggaa attaaggaaa
     3961 gtataatact tttaagcatg attcagcttc caccttacct gcaaaaagga gataccatcg
     4021 ggctggtatg ccccgccggc tatatgcctt tcgaaaaagt gaaaacctgt gtgcagatcc
     4081 tgcaggaatg ggggtacaat gtaaaagtgg gcaataccgt tggcggtggt tccatgacct
     4141 atttctccgg aacggatgac gaacgactgc gcgattttca gcagatgctg gatgataatg
     4201 aagtgaaagc agtactctgt gcccgaggcg ggtacggcac cggacggatc attgaccgga
     4261 ttgattttaa aaaatttcaa aagcaaccca aatggattgt cggttacagt gatatcaccg
     4321 tacttcattc ccatctcttc agtaattatt atatcgcctc cctgcatgcg ccgatggcgg
     4381 gagccttcaa tgaagaagga tttaagaatg agtatgtgct atccctgaaa aataacctcg
//
LOCUS       DAOPMW010000274         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23311_length_4401_cov_6.080304, whole genome shotgun sequence.
ACCESSION   DAOPMW010000274 DAOPMW010000000
VERSION     DAOPMW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23311_length_4401_cov_6.080304"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLU11_11760"
     CDS             <1..356
                     /locus_tag="PLU11_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HPN59847.1"
                     /translation="WTEHEIKQAKHMKETFKEVMHNMGAIITWGGDDNESNNWGLSKP
                     GEIIHEAGTVRMGNDPKTSALNKWSQAHDCKNLFCVDGGQFVSQADKNITWTILALSM
                     RACEYIVEEMKKQNL"
     gene            372..1040
                     /locus_tag="PLU11_11765"
     CDS             372..1040
                     /locus_tag="PLU11_11765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HPN59848.1"
                     /translation="MDRRKSLKLIATGAVAVPAALAGCATDDKKVADKAAEPVFNLDR
                     NPDEVKYEKELLAKGPFFNAHELATITVLCDIIIPKDEVSGSASEAGVPDFIDFIVRD
                     MPNHQVPLQGGLRWLDLQCFKRYEKPFKDCTAAQQIELVDMIAYPEKAKPEMSQGVQF
                     FNLMRNLTASGFYTSAIGIKDIGYAGNQPNQWNGVPPEVLAQYKLAYTEKELKECISF
                     DKEA"
     gene            1080..2261
                     /locus_tag="PLU11_11770"
     CDS             1080..2261
                     /locus_tag="PLU11_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59849.1"
                     /translation="MKKYALLAVVFLYTFLLSAQSYVTIHEDCDYRGKSYTLEAGTYR
                     LYQMRIGNDKLSSFQVPSGFRITIYENDDFNSRSKTFTESVSCLDSAWDNNASAIVVE
                     NTNIQQTNPNEYVVIYSDCYSRGFSRSLRPGVYKGTDLGELKNNISSFAIFGNLRMRI
                     YTGSDDASGYFVTLENSETCLSRNYNDRISSLVVEYKPYTTPGTGNNSNNGSYAEFYT
                     DCNYKGNKLSLLPGRYTGEELGLFRKTIGSMQIPAGISVKVYEGDNLMGASETYSSGF
                     DCISYSWRNRISSLVVEEKGGWSGNGNPMVNELTLYTDAGYKGQSVTLLPGTYKTMAA
                     ANGFPEKALSSIQIPPGYRVVLYDQPNLRGKSMTLTASRSSFAMSSWNDRAASIAVYR
                     N"
     gene            complement(2315..>4401)
                     /locus_tag="PLU11_11775"
     CDS             complement(2315..>4401)
                     /locus_tag="PLU11_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPN59850.1"
                     /translation="AGSGKTTLAECMLFEAGIITRKGSIAERNTTGDYHELEQERGNS
                     IFSKLLHTKWRGYKINIIDTPGYDDFSGEVISALRVADTGVMLINAVMGVEVGTDIIW
                     EYTEQFKTPMIFGVNKLDDDNADFEKTIREAKSHFGNNVVVVQYPRQTGAGFHEIIDV
                     LRMTMYHFKDNGGKPEKMAIPDEEKAKAEELHKELIEAIASNDETLMEKYFDKGELDE
                     DEMKNGLKKAMINHDLFPLFCLSAERNMGSGRLMGFIDNVCPSANEMPPQTTKKGEAL
                     PCDANGPACIFIYKTISEPHVGELSFFKVYSGTIKSGMELENETTGVTEKITQLFLVE
                     GNKRTPINELVAGDIGATLKLKNTHVNNTLHTKGKNFELPPIVFPAPNMTVAIETLKK
                     GEEEKLSLALHQLREEDPTLLVEVSSELKQTLIHCQGDMHLAVAKWKIEHQHKLEVKF
                     SKPRIAYRETIRKMADANYRHKKQSGGAGQFGEVYMRIEPWYEGMPEPDDLNVRGRDI
                     HELDWGGRLVFYNCIVGGAIDARFLPSVLKGVMEKMHNGPLTGSYARDIRVCVYDGKM
                     HPVDSNDISFKIAGLQAFRQAFQQADPQILEPIYKLEVLCPDDLTGAVMGDLQSRRAI
                     VEGIDSEGHFQKVIAKVPLAEMDGYSSSLRSITQGRAKFKMAIAGYEAVPFDLQRKLI
                     DEYSKTAKEEFA"
BASE COUNT         1152 a         1018 c         1034 g         1197 t
ORIGIN      
        1 agtggaccga gcacgagatc aagcaggcaa aacacatgaa ggaaaccttt aaggaggtga
       61 tgcataatat gggtgccatc attacctggg gtggtgatga taatgaaagc aataactggg
      121 gactttccaa acccggtgag atcatccatg aagccggaac cgtccggatg gggaatgatc
      181 ccaaaacctc ggccctgaat aaatggagcc aggcccatga ttgtaaaaac ctgttctgtg
      241 tggatggcgg tcagtttgta agccaggcgg ataaaaatat cacctggacg attctcgctt
      301 tatccatgag agcctgtgag tatattgtag aggaaatgaa aaagcaaaac ctgtaattga
      361 aatactattt tatggacaga agaaaatcgt tgaaactgat tgccaccgga gccgttgcgg
      421 ttcctgctgc tcttgccggt tgtgccacgg atgataaaaa agtagcggat aaagcagctg
      481 agccggtctt taacctggac cggaatccgg atgaagttaa gtacgagaaa gagctattgg
      541 caaaaggacc tttttttaat gcccatgaac tggccaccat taccgtcttg tgtgatatca
      601 tcattcccaa agatgaagtc agcggcagtg ccagtgaagc gggcgtaccg gattttattg
      661 attttattgt aagagatatg cccaatcacc aggtgccctt acagggcggc ttgcgctggc
      721 tggatctgca atgcttcaaa cgatatgaaa agccctttaa agattgtacc gccgcacagc
      781 agatcgaact ggtggatatg attgcctatc cggagaaagc aaaaccggag atgagtcagg
      841 gggttcagtt tttcaacctg atgcgaaatc tgaccgcctc tggtttttac acatcagcga
      901 ttggcatcaa agacattgga tatgcaggta accagcccaa tcaatggaat ggagtgcctc
      961 cggaagtgct ggcacagtat aaactggctt ataccgagaa agagctgaaa gaatgtatta
     1021 gttttgacaa ggaagcctga tccggaatga cggtaaggcg gtctgctaaa atttttgcta
     1081 tgaaaaagta tgccttactc gcggttgtat ttttatatac atttctgtta tctgcccaga
     1141 gttatgtaac tattcatgag gattgtgatt atcgtggaaa gagttatacc ctggaagcag
     1201 gcacctaccg tttataccag atgcggatcg gcaatgataa actgtcctcc ttccaggtgc
     1261 cgtctggttt ccggattact atttatgaga atgatgattt caacagccgg tcaaaaacat
     1321 tcaccgaatc ggtcagttgc ctggattcag cctgggataa taatgcatcc gccattgtag
     1381 tggagaatac caatatccag caaaccaatc ccaatgagta tgtcgtgata tacagcgatt
     1441 gttattcccg tggtttctcc cgcagtcttc gtccgggggt atacaaaggc acagacctgg
     1501 gtgaattaaa aaataatatt tcctccttcg ccattttcgg caatctgcgg atgcggatct
     1561 ataccgggtc agatgatgcc agtggttatt ttgtaaccct ggaaaacagt gaaacctgcc
     1621 tgagccggaa ttataatgac cggatcagtt cattggtggt ggaatataaa ccatatacta
     1681 caccgggtac cggtaataac agtaacaatg gatcttatgc cgaattttat acggattgca
     1741 attacaaagg aaataaacta agcctgcttc ccggacgata taccggggag gaactgggtt
     1801 tattccggaa gaccatcggc tccatgcaaa tccctgccgg aatttctgtt aaagtgtatg
     1861 aaggagataa tctcatgggc gcttcagaaa cttattcttc cggatttgat tgtatcagtt
     1921 acagttggag aaaccggatc agttcactgg tggtggaaga aaaagggggc tggagcggta
     1981 atggtaaccc gatggtgaat gaactgaccc tctacacgga tgcggggtat aaaggacaat
     2041 cggttacctt attaccgggt acttataaaa caatggccgc tgcaaacgga ttccccgaaa
     2101 aagccctcag ctccattcag atccccccgg gataccgggt agtactttat gaccaaccca
     2161 atctcagggg caagagcatg acgctgacgg cttccaggag tagttttgca atgtccagct
     2221 ggaatgaccg ggctgcttcc atagctgttt atcgcaatta ataaaaaacg ccggtcagta
     2281 atctggccgg cgtcaaagca tagttggtta cgacttacgc aaactcttct ttagcggttt
     2341 tgctgtattc atcgatcagt ttccgttgca ggtcaaatgg cacggcttca taacccgcaa
     2401 tagccatttt gaatttagcc cgtccctggg taatggaacg aagggatgag gaataaccat
     2461 ccatttcagc caacggtact ttagcgatca ctttctggaa atgcccttcg ctgtcgattc
     2521 cttccacgat ggcccggcgg ctttgcaaat cgcccattac ggcgccggtc aggtcatccg
     2581 gacaaagtac ctccagttta taaataggtt ccagaatctg aggatccgcc tgctggaaag
     2641 cctggcggaa tgcctgtaag cccgcaattt tgaaagagat atcattgctg tccaccgggt
     2701 gcatctttcc atcatacaca caaacacgga tatcgcgtgc ataagaaccg gtcagtggcc
     2761 cgttatgcat tttctccatg actcctttca aaacggaagg cagaaaacgg gcatcaatag
     2821 ctccacccac gatacagttg taaaatacca gacgtccacc ccaatcgagt tcatggatat
     2881 cccggcctct tacattcagg tcatccggtt ccggcatacc ttcataccag ggctcaatac
     2941 gcatatagac ttctccaaac tgtccggcac ccccactctg ttttttgtgg cgataattgg
     3001 catcggccat tttacggatg gtttcacgat aagcgatacg cggcttgctg aatttaactt
     3061 ccagtttatg ctggtgttca attttccatt tggctacggc caggtgcatg tccccctggc
     3121 aatgaatcag ggtctgtttc aattcggatg aaacctccac cagtaatgtg ggatcttctt
     3181 cccgcaactg gtgcagggcc agggagagtt tttcctcttc tccctttttc agtgtttcaa
     3241 tcgccacggt catattgggc gcagggaaca caatcggagg aagttcaaag tttttaccct
     3301 tggtatgaag cgtattgttc acatgggtgt ttttgagctt cagggtagcc ccgatatcac
     3361 cggccaccaa ttcattgatc ggtgtccgtt tatttccttc caccaggaat aactgggtaa
     3421 tcttttcggt aaccccggtg gtttcatttt ccagttccat tccgcttttt atggtacccg
     3481 aatacacttt aaagaaagaa agttcgccca catggggttc tgaaatggtt ttataaatga
     3541 aaatgcaggc gggtccgttt gcatcacagg gaagtgcttc gccttttttg gtggtctgcg
     3601 gtggcatttc gttggcactg ggacaaacat tgtcgataaa acccatcagg cggccactgc
     3661 ccatattgcg ttcggcagac agacagaata acggaaacag gtcatgattg atcatggctt
     3721 tcttcaggcc attcttcatt tcatcttcgt ccagttctcc tttatcgaag tatttttcca
     3781 tcagggtttc gtcattgctg gcaattgctt cgatcagttc cttatgcagt tcttccgctt
     3841 ttgctttttc ttcatcgggg atggccattt tttcgggttt cccgccattg tctttgaaat
     3901 ggtacatggt catccgtaat acatcgataa tttcatggaa gccggcaccg gtctgccggg
     3961 gatactgcac caccactaca ttattaccaa aatgcgattt ggcttcccgg atggtctttt
     4021 caaaatccgc attatcatca tccagtttat tcaccccgaa aatcattggt gttttaaact
     4081 gttcggtata ttcccaaatg atatctgttc ctacttccac acccattacg gcattaataa
     4141 gcatgacacc ggtatcggcc acgcgaaggg cggagatcac ttcaccggag aagtcatcat
     4201 aaccgggggt atcaataata ttaatcttgt atcctctcca ttttgtatgg aggagcttgc
     4261 tgaaaataga attaccacgt tcctgttcaa gttcgtggta atcacctgtt gtattccgct
     4321 ctgcgataga acccttccgg gtaataatgc ctgcttcaaa cagcatgcac tccgctaagg
     4381 tggtcttacc ggagccggca t
//
LOCUS       DAOPMW010000275         4391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23386_length_4391_cov_10.168127, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000275 DAOPMW010000000
VERSION     DAOPMW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23386_length_4391_cov_10.168127"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PLU11_11780"
     CDS             <1..2055
                     /locus_tag="PLU11_11780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN59851.1"
                     /translation="ISINKDHTINLIVLQHEFGLFKDCRDEFVNFLGMLKKPLLIVFH
                     TVLPDPGDSLRAQVRLLADVSNRVMVMTNKSSDILIKDYGLEKYKITMIPHGTHLVLH
                     TDCETLKRKYKLTGRKVLTTFGLLSSGKGIETTLNALPEIVASDPEVIFLVIGKTHPA
                     VIRSEGEVYRQLLEKKVTELGMTKYVRFINYFLPLGELLEYLQLTDVYLFTSQDRSQA
                     VSGTFSYAISCGCPVVSTPIPHAVEVLKNDGGIIIDFGNSAQLGAAVTSLLQNKQLRA
                     NIVLNGLHRMAPTAWENAAIAHAFLFQEMLHGKIDLQYKIPPLNTAHIREMTTSTGIY
                     QFSVLHNPDPADGYTLDDNARALVATCQHFEKTGDPEDLEYISIYLQFIRLCQQPDGS
                     FLNYMNQEGQFTKQNDEVNLDDANGRAVWALGYVVSRCTILPEKFSTLAFTILQQRLA
                     TCEPIHSTRAMAFIIKGLYYKTTSYTDQDDVELIRLFADRLVQMYLHETDRDWHWFEG
                     YLTYANSLLPEALLCAWLATGNMIYKEIAKKSFDFLLAKIFHGNRITVISNHKWLHDR
                     LVSEVETTGGEQPIDVAYTIMALKRFYTVFRDPGYYDKMQASFNWFLGENHLHQIIYN
                     PCTGGCYDGLESDYVNLNQGAESTVSYLMARLTIDEDALKRRLPEEEEILLNEIYETQ
                     SV"
     gene            2150..2350
                     /locus_tag="PLU11_11785"
     CDS             2150..2350
                     /locus_tag="PLU11_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein CsbD"
                     /protein_id="HPN59852.1"
                     /translation="MKNTDGTSLAEAGSWEDMKNKLKAKFSWLTDADLQYTEGKKEEM
                     MNRLQIKIGKTKDELNKIFAGL"
     gene            complement(2478..>4391)
                     /locus_tag="PLU11_11790"
     CDS             complement(2478..>4391)
                     /locus_tag="PLU11_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN59853.1"
                     /translation="VNEEKYRSIIANMNLGLLEVNMEEVIQFANNSFCEMSGYSLEEL
                     IGKKAAELFVRGDNTEIAETKNELRKEGISDAYEIGVTNKKGEQKWWLISGAPRFDDQ
                     DNLVGSIGIHLDITAQKKLELELLDARESAEQSAAAKEIFLANMSHEIRTPMNAIIGM
                     GRQLHRTILNDQQQFYLETINKAAEHLLVLINDILDISKIEAGKLNLEQIGFRPDEVI
                     DHCIQVMEHKAEEKGLKLMKENSPEKSTIFLGDPYRLTQILLNLLSNAIKFTEEGNVT
                     ISCLLQPVINDRQLIILSVKDTGIGMDGEFLSSIFRKFTQEEQSTARKYGGTGLGMSI
                     SKQLTELMNGHIEVSSKKGEGTTITLTIPFSVGTEKDIPQTKGKVIDSSILKEKKILL
                     VEDNEMNRLVATTLLENYGVNIAEVHDGAEAVRALKTGYYDLVLMDVQMPVLNGLDAT
                     RIIRQEVNQFVPIIALTANAIKGEAEKCLKAGMNDYISKPFEEEELINAIARWLGEEP
                     ESFTTLQPSAMENKPAIPLYDLKKLEQIAQNNQPFVLKMVTLFSRQAPADVHEIEMAF
                     EKQDYHTVRSVAHRMKPSIDNMGILSLHDLIREIELFNQTEKTTDQLENRIRELRDTI
                     TQVVDLLQKEVLI"
BASE COUNT         1146 a          936 c         1009 g         1300 t
ORIGIN      
        1 atttctataa acaaggatca tacgatcaat cttattgtat tacagcatga gtttggcctg
       61 tttaaggatt gccgcgatga gtttgtcaat ttccttggaa tgcttaaaaa gccgcttttg
      121 attgtttttc ataccgtatt gcctgatcca ggtgattcac ttcgggcaca ggtaaggctt
      181 ttggcagatg tcagcaaccg ggtaatggta atgacgaata aatcatcgga tattctgatt
      241 aaggattacg gcctggaaaa atataaaatt accatgatac cgcatggcac tcacctggta
      301 ttgcatacgg actgtgagac attgaagcgg aagtataaac taacgggcag gaaagtgtta
      361 accacatttg gattgctcag ttcaggaaaa ggtatagaaa ccaccctgaa tgccctccct
      421 gaaattgtgg cgtcggaccc ggaggtaatc tttctcgtga ttggcaagac tcatccggca
      481 gttatacggt cggaaggaga agtataccgg caactgcttg agaagaaagt gacggaattg
      541 gggatgacta aatatgtccg gtttattaat tattttcttc cgttgggaga actgctggag
      601 tatttacaac tgaccgatgt atacctgttt acctctcagg atcgcagtca ggcagtgagt
      661 ggtaccttct catatgcaat cagctgcggg tgtccggtgg tttccacccc gattccacat
      721 gccgttgaag tattaaaaaa tgatggcggg ataattatcg atttcggaaa ttcggcccag
      781 cttggggcag ccgttacttc tttattgcag aataaacagt taagagcgaa tattgtttta
      841 aacggacttc accggatggc tcctacagcg tgggaaaatg cggcgattgc ccatgctttc
      901 ctgttccagg aaatgctgca tggaaaaatt gacctgcagt ataagatccc gccactcaat
      961 accgcccata tccgggagat gaccaccagc acaggaattt accagttttc cgttttgcat
     1021 aatccggatc cggcagatgg ctatacactg gacgataatg cgagggcgct ggtagcgacc
     1081 tgccagcatt ttgagaagac cggtgatccg gaagacctgg agtatatcag tatctacttg
     1141 cagtttattc gtttatgtca gcagccggat ggctcgtttc tcaattatat gaaccaggaa
     1201 ggccagttta ccaaacagaa tgatgaagta aatctggatg atgcgaatgg aagggccgtc
     1261 tgggccctgg ggtatgtagt gtcccgttgt accatattgc cggaaaaatt ttcaacgctg
     1321 gcatttacaa tccttcagca acggcttgct acctgtgagc ctatacattc caccagggct
     1381 atggccttta ttattaaagg cctttactat aaaacaacca gttacactga tcaggatgac
     1441 gtggagctga tccgtttatt tgccgaccgg ctggtgcaga tgtaccttca tgaaactgac
     1501 cgggactggc attggtttga aggatacctg acctatgcca acagtctgtt accggaagcc
     1561 ctgctttgtg catggctggc cactggaaat atgatttata aagagattgc taaaaaatct
     1621 tttgattttc ttttggcaaa aatattccac ggaaacagga tcactgtgat ttccaatcat
     1681 aaatggttgc atgaccggtt ggtttcggaa gtagagacaa ccggtggtga acaaccaatc
     1741 gatgtggcat atacgataat ggccctaaaa agattttata cggttttcag ggacccggga
     1801 tattatgata aaatgcaggc ctcctttaac tggtttttgg gggagaatca tttgcaccag
     1861 attatttata atccttgtac cggtggttgt tacgatggac tggaaagcga ttatgtaaac
     1921 ctcaaccagg gagcggaatc aacggtaagt tacctgatgg cccggctgac gattgatgag
     1981 gatgcgctaa aacgccgact gccggaggag gaagaaatac tgttgaatga aatctatgaa
     2041 acccaatctg tgtaactggt aattatgtaa aaggttggca aagagttgta acaagccaac
     2101 agtataattt acagaactta cactgagtaa aataatttaa atcccgaaaa tgaaaaatac
     2161 tgatggtacc tcattggccg aagctggaag ctgggaggat atgaagaaca aactcaaagc
     2221 taaattttcg tggctgaccg atgcggatct tcagtatact gaaggcaaaa aagaagaaat
     2281 gatgaaccgg cttcagatta aaatcgggaa gacaaaagat gaattaaata agatttttgc
     2341 cggattatag caatatccgc attctatact ctgaatttat cagggacggt cacagttatt
     2401 ttaaacggta gttataagca aaaagaggat tccggtaaaa ctcatcatga aaatggtgag
     2461 ttttttttgc agccgggtta tatcaatact tctttttgca gtaagtccac tacctgagtg
     2521 atcgtgtccc gcaactccct gatcctattt tctaattggt ctgtagtctt ttcggtttga
     2581 ttaaataatt caatttccct tattaaatcg tgtaaagaca aaatgcccat attgtcaata
     2641 gagggtttca tccggtgggc aactgaacgg accgtgtggt aatcctgctt ttcaaaggcc
     2701 atttcaattt catggacatc ggccggagcc tgccggctga acaaggtgac cattttcagg
     2761 acaaatggtt gattgttctg ggcaatttgc tccagttttt tcaggtcgta taaagggatg
     2821 gcgggtttgt tttccatggc agaaggttga agagtggtaa aagattccgg ttcttcccca
     2881 agccacctgg cgatagcatt gatgagttct tcttcttcaa atggtttgga gatatagtca
     2941 ttcattcctg ctttcaggca tttttcggct tcccctttaa tggcattggc cgtcagggca
     3001 ataatcggaa caaattgatt gacctcctga cggatgatcc gggtggcatc cagtccgttc
     3061 agtaccggca tctgaacatc catcagtacc aggtcgtaat acccggtttt gagcgccctt
     3121 actgcttctg ccccgtcgtg cacctctgcg atatttaccc catagttttc aagcagggtg
     3181 gtggcgacca gcctgttcat ttcattgtct tccaccagca ggattttctt ttctttcagg
     3241 atagaactgt caatcacttt tccttttgtc tgcggtatat ctttttccgt accgacagaa
     3301 aacgggatgg tgagggtgat tgtggtgcct tcccctttct tactggatac ttcgatatgt
     3361 ccgttcatca gttcagtgag ttgtttactg atggacatcc ccagccctgt tcctccatat
     3421 tttctggcgg tggattgttc ttcctgggtg aatttccgga agatcgagga aagaaattct
     3481 ccatccattc cgattccggt gtcttttacc gacaggatga tcagttggcg gtcattgata
     3541 acgggttgca gcaggcagga aatagtgaca ttgccttctt ctgtgaattt aatcgcatta
     3601 ctcagcaggt tcaggaggat ctgcgtaagc cggtaggggt caccgaggaa aatcgttgat
     3661 ttttccgggc tgttttcctt cattaatttc aacccctttt cttccgcttt gtgttccatc
     3721 acttgtatac aatggtcaat cacttcatcc ggacggaagc cgatctgttc caggttcagt
     3781 ttacccgctt ctattttgga aatatctaat atatcgttga tcagcaccag caggtgttct
     3841 gcagctttat tgattgtttc caggtaaaat tgttgctgat cgttcagtat tgtccggtgc
     3901 aactgccgtc ccataccaat gatcgcattc attggcgttc ggatctcgtg gctcatattg
     3961 gccaggaaaa tttctttagc agcggcagac tgttctgcgg attcacgggc atcgagtaat
     4021 tccagttcca gttttttctg cgctgttata tccaggtgta taccaataga gcccaccagg
     4081 ttatcctggt catcaaatcg gggagcacca ctgatcagcc accatttctg ttctcctttt
     4141 ttattggtga caccgatttc gtaggcatca gaaatgcctt ctttacgtaa ctcattcttc
     4201 gtttctgcaa tttcagtatt atcgccacgc acaaaaagtt cagctgcttt ttttccgatc
     4261 aattcctcca ggctatagcc cgacatttca caaaaactat tattggcaaa ctggatcacc
     4321 tcctccatat ttacttcgag cagccccagg ttcatgttgg caataatgct ccggtatttt
     4381 tcttcattaa c
//
LOCUS       DAOPMW010000276         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23461_length_4380_cov_9.636532, whole genome shotgun sequence.
ACCESSION   DAOPMW010000276 DAOPMW010000000
VERSION     DAOPMW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23461_length_4380_cov_9.636532"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLU11_11795"
     CDS             complement(<1..645)
                     /locus_tag="PLU11_11795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN59854.1"
                     /translation="MQKFISSLVVLGLFTFSSCSKSKGSGAPPPDTTQKDISAIDNAV
                     QNFMTAYNIPGTSIAITKDDKLVYVKSYGQISATDNTPINNNNLFRIASVSKPITAVG
                     IMKLLEANKLTLDSKVFGTGSILGNDFPSTQLAAVSDITVRHLLHHTSNAWPNDGNDP
                     MFKQPTYNFSQLISWTLNNYNAAVTRGVYKYSNFGYCLLGRIIEKLSGKSYEQFI"
     gene            complement(677..1273)
                     /locus_tag="PLU11_11800"
     CDS             complement(677..1273)
                     /locus_tag="PLU11_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPN59855.1"
                     /translation="MKKVILLLIVICITRFSSIAQLSYGVKAGVNLAFISFSDEKYNT
                     HMKPGILAGGFAHYPLNAKTGLQAELLFSTEGNKWDTKPASVTGIIKSSQVRIPVLIQ
                     YKIHPRFALEAGPQFRWLITMKQTAVGDTYSIKPLYYTGALGLAAGASFQLPGKFPGL
                     KAGFRINRELSKINKQDVGGGTLHNTVMALSLAYTFKK"
     gene            complement(1429..>4380)
                     /locus_tag="PLU11_11805"
     CDS             complement(1429..>4380)
                     /locus_tag="PLU11_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPN59856.1"
                     /translation="EARKASPRTLVKACREAAEFFANNGQNSEGYRSCELSLDYAQEL
                     TERKELEIAQCEYNLGVYAQRLANYNLSQSHHRRSLLIRQTIKGATPEDLYASFNAMG
                     GIYWYASKYDSAALCYTSALENVKKMPENDLNRLYRPAMIYNNLAALQSAEGKTSEGI
                     RNTELTISYFQQFIATKDPHPKKKDAVSGLFAAIDNLAGMYKEVGDYGKAGDLLRYSY
                     QEKLKRLNTGNPGIFISEILIGQHYNSIHEYDSALIYLTRGLEKLEKAEGDYLFWAGD
                     AHYNLAMVFANRKETAKATYYYEKAEAYYDSSYQGDYDNIIMDFLRNAAIFYAKNNQP
                     DKALSRAKKVYAYLVKVGQAKSLQAFYQLLNLAEVSYLTTQYNEALNYSGQALKVVNE
                     KVTAGMTLLDSIKIDVFKPKAILLQEKAGYALQKTHDTVYLSAMASRLNQALQILEKR
                     KVLIDDAESINILIADNTELIDFTKQIELELYEKSGQTAHLDRFINLHESGLYNRIRS
                     RLDKKRAIRFSNLPLNIQEEEQQLKAAITTSLNRDKSNSELIQDYLTAVKNWEAFLVK
                     VKEQYPVYYTMRYASIFRSLPEMQSALPANTTLVRYYFTGVRLVVLVADSVSKKLVSL
                     DNNNLESQIAGLLQQGYQEKPAAATLSSLYQTLWAPLAPAVSTKKVLIIPDGILFNFS
                     FELLTPTAIRTYSELAGNSLLAKHEITYHYSLFMSGQQQAEDTLNQNYVAFAPGFSDE
                     LKKEYVESVNDSLKLDMQYLSLLPQPNTGKLVKKFKSLLGGQTYLNRSSTPAEFRKNA
                     GGHKIIHIGTHAEYNNINPERSRLIFAKTPLVSADTNSLFLYDIYNCNVSSDLTLLTA
                     CETGRPGYQDGEGMVSLAHAFNYAGSRSILTGLWKIDEQSSSQITESFIRHLKEGLPS
                     DEALRQAKLDYLSGNPERLLAPAYWSGLVLMGKPGSIQFDNPGLSPWWWVLAGTGILA
                     ILLFLRRKFSR"
BASE COUNT         1068 a          926 c         1120 g         1266 t
ORIGIN      
        1 aataaactgc tcatagcttt taccagaaag cttttcaatg atgcgcccga gcagacagta
       61 accgaagttg gagtatttgt aaacaccccg ggtgacggcg gcattataat tattcagggt
      121 ccagctgatc agctggctga agttataggt tggttgcttg aacatgggat cattgccatc
      181 attgggccag gcattgctgg tatgatgcag gaggtgacga acggtaatat cactgacggc
      241 tgccagttgt gtagacggga aatcattacc gaggatacta cctgtgccga agactttgga
      301 gtccagggtg agtttatttg cttccagcaa tttcatgatt cccactgcgg taatgggttt
      361 ggaaacactg gcaatccgga aaaggttatt gttgttgatt ggggtattat cggtggcact
      421 gatctgtcca tacgatttta catagaccag tttgtcatct tttgtaatgg caatggatgt
      481 gccggggata ttataagcag tcatgaagtt ctgtacagcg ttatcaatag ctgaaatgtc
      541 tttctgagtc gtatccggtg gcggggcgcc tgaccctttg ctttttgaac agctgctgaa
      601 ggtgaacagg ccaagaacca cgagggatga aataaacttt tgcattacat tggttttggg
      661 atgaatgaag aggtgattat tttttaaatg tataagcgag cgacagggcc attacggtat
      721 tgtgcagggt accaccgcct acatcctgtt tatttatttt ggagagttcc cggttgatcc
      781 gaaaaccggc tttcaatccg ggaaattttc cgggaagttg aaaactggca ccggcggcta
      841 atcccagtgc cccggtataa tacaagggtt ttatactgta tgtgtcacca acagctgtct
      901 gtttcatggt gatcagccag cggaattgcg gacctgcttc aagagcaaag cgtggatgaa
      961 ttttatactg aattaatact ggaattctga cctggcttga tttgatgata cccgttacgg
     1021 aagcaggttt tgtatcccat ttattccctt cggtggagaa gagcagttcc gcctgcaggc
     1081 cggtttttgc atttaacgga taatgggcaa aaccacccgc caatattccg ggtttcatat
     1141 gggtattgta cttttcatcg ctgaacgata tgaaagccag gttaacaccg gcttttacgc
     1201 catagctaag ctgggcaatg gaagaaaacc gggtaataca tattacaata agcagaagga
     1261 tgactttttt cattagtgaa atcatttttt agttgaggtg aaaaggcgat ctctgacagt
     1321 acatcggtgg aggagcagaa aggtcatcag ccggacagtg gctgatagaa ataaaaaagc
     1381 ctgatctgta atgtatacag atcaggcccg taaatcagga aaggggtatt aacggctgaa
     1441 ttttctgcgc aaaaagagga gtattgccag gatacctgtt ccggcaagca cccaccacca
     1501 gggactcagt ccggggttat caaactgtat ggaccccggc tttcccatca acaccagtcc
     1561 gctccagtat gcgggtgcca gcaaacgttc cggattacct gacaggtagt ccagttttgc
     1621 ctggcgaagt gcttcatcgc tgggtagtcc ttctttgagg tgacggataa atgactccgt
     1681 gatctggctg ctgctttgtt catctatttt ccataaaccg gtcaggatac tccggctgcc
     1741 ggcataatta aaagcgtgtg cgagcgagac catgccttcg ccgtcctggt agcccggtcg
     1801 cccggtttca caggcggtga gcagggtaag gtcggagctt acattacagt tataaatatc
     1861 atagagaaaa agtgaattgg tatcggccga aacaagcggt gttttagcaa agatcagccg
     1921 ggagcgttcc gggttgatat tgttgtattc cgcgtgggtg ccgatatgga taatcttatg
     1981 gccaccggca tttttcctga attcagccgg ggtggaagac cggttgagat aggtttgtcc
     2041 cccaagcagg gatttaaatt tctttaccag tttgccggta ttcggctgag gcaatagaga
     2101 aaggtattgc atatcgagtt tcagggaatc gtttaccgat tccacatatt ctttctttaa
     2161 ttcatctgag aatccggggg caaaggccac ataattctga ttgagggtat cttctgcctg
     2221 ctgttgaccg gacataaaca ggctgtaatg ataagtgatt tcatgtttgg ccagcaggct
     2281 gttgccggcc agttcactgt acgtccggat ggcagtgggt gtgagtaatt caaaactgaa
     2341 attaaaaagg ataccatcag gaatgatcag cacttttttt gtggaaacag caggggccag
     2401 tggggcccag agtgtctggt acaaagagga gagggttgca gcagctggtt tttcctggta
     2461 accttgttgt aataagccgg ctatctggct ttcgaggtta ttgttgtcca gggaaaccag
     2521 tttctttgaa acactatcgg ccaccaggac tactaatcgt acgccggtaa agtaataccg
     2581 gaccagtgtg gtatttgcag gcagggcaga ttgcatttca ggtagcgagc ggaaaatgct
     2641 ggcataccgc atggtataat aaaccgggta ttgttctttt acttttacca gaaatgcttc
     2701 ccagtttttt acagcggtca ggtagtcctg aatgagttca ctgttggatt tatcccggtt
     2761 cagggaggtc gtaatggcgg ccttcaactg ttgttcttcc tcctgaatgt tcaggggcag
     2821 gttgctgaac cggatagctc tttttttatc aagccggctg cggatccggt tataaagtcc
     2881 ggattcgtgc agattgataa agcggtcgag atgggcagtt tgtcctgatt tttcatagag
     2941 ctcaagctcg atctgtttgg tgaaatcaat cagttcggta ttgtccgcaa tcaggatatt
     3001 gatgctttcg gcatcatcga tcagcacttt tcttttttca aggatctgta aagcctggtt
     3061 caggcgggat gccatcgcag acagataaac ggtatcatga gttttttgca aggcataccc
     3121 ggctttctcc tgtaaaagaa tggctttggg tttgaatacg tcaatcttaa tcgaatccag
     3181 cagggtcata cccgccgtta ctttttcatt caccactttc agtgcctgtc cgctgtagtt
     3241 cagggcttcg ttatattgag tggtcaggta actgacttct gccaggttaa gcagctggta
     3301 aaaggcctga aggctttttg cctgacctac tttcaccagg taggcatata cttttttcgc
     3361 ccggctgagt gctttatcag gttggttgtt cttggcataa aagatagccg cgtttcgcag
     3421 gaaatccatg atgatattat cataatcacc ctggtaggag gaatcgtaat aggcttctgc
     3481 tttttcataa tagtaagtgg cttttgccgt ctctttccgg ttggcaaaga ccatggccag
     3541 gttataatgc gcatctccgg cccagaacag gtaatcccct tccgcttttt caagtttttc
     3601 aagtccacgg gtcaggtaga tcagggcaga gtcgtattca tggatcgagt tataatgctg
     3661 tcctatcagg atttcggaaa taaaaatacc cgggttaccg gtattcagtc ttttcagttt
     3721 ttcctggtac gaataacgga gcaggtcacc cgcttttcca taatcaccca cttctttata
     3781 catgccggcc aggttatcaa tggctgcaaa gagcccagat actgcatctt tctttttagg
     3841 atgcgggtct ttggtggcga taaactgctg aaaatagctg atggttaatt ccgtgttacg
     3901 tatgccttcc gaagtctttc cttctgcact ttgcagggcc gccaggttgt tatagatcat
     3961 ggcaggacgg tatagccggt tcaggtcatt ctccggcatt tttttgacat tttccaatgc
     4021 gctggtataa cacaaggcag cactgtcgta tttggaagca taccagtaaa tcccccccat
     4081 ggcattgaag gaagcataga gatcttccgg agtggcacct tttattgttt gacgaataag
     4141 gagcgagcgg cggtgatggc tttgtgaaag attgtaattc gccaaccgct gggcatacac
     4201 cccaagatta tattcacact gggctatttc cagttctttc cgttccgtca gttcctgggc
     4261 atagtccagt gataactcac aggaacggta tccttcactg ttctgaccat tattggcaaa
     4321 aaattcggcg gcttcccggc aggcttttac cagtgttctg ggtgatgctt tccgggcttc
//
LOCUS       DAOPMW010000277         4378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23482_length_4378_cov_9.391858, whole genome shotgun sequence.
ACCESSION   DAOPMW010000277 DAOPMW010000000
VERSION     DAOPMW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23482_length_4378_cov_9.391858"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLU11_11810"
     CDS             <1..373
                     /locus_tag="PLU11_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPN59857.1"
                     /translation="VSFLDKTMIPHLLQEEEVIFPYIRQISHAYESKESYASLLVRTL
                     RKPVEDLMHDEHNSIGKTLTQLRELTNNYTAPENACTSHRLAYSLIKELDDDLVQHIY
                     LENDILFPKAISMEKELLERH"
     gene            424..1989
                     /locus_tag="PLU11_11815"
     CDS             424..1989
                     /locus_tag="PLU11_11815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPN59858.1"
                     /translation="MDNLNGRSMTYWAILVFSFSLTLAFLFRDYQLSVSGLLLFVILT
                     AFIPGWRSTLAAGVISMVVMTALVIYFKKDDDDITRALLSQIYSLFVVVFSVVVIFYL
                     KKMQQNFAHEKTHMASLFENATEGILLTDKSGSIVLVNPAAERMFGYGAEELIEEKIE
                     KLIPMRFLDRHVGLREGFYKHPSNRTMGSGRDLYARRKDGSEFPVEVSLSHYKQKDED
                     FVIGFIVDITARKEIEKNLLRQKMELEKISDDIRKLNTDLEGKVEERTIILKEALQKL
                     EESQQELSESLDKERQLNEIKSRFVSMASHEFRTPLSTILSSATLVSKYPSGEEFDKR
                     EKHIRRIKDSVNHMNELLEDFLSLGKLEEGKVGVTVTVFSVKDFVEDVVDEMKAHLKS
                     GQEIVLDCIGDGNFKTDKRMLKNIMLNLISNAIKFSGEGKKVWLCTNTVNSVLNISVR
                     DQGLGIPEEDQPHLFSTFFRAKNVSNIQGTGLGLPIVKRYVNLLGGDIHLNSELEKGT
                     EVVISLPEYKEPD"
     gene            complement(2030..2731)
                     /locus_tag="PLU11_11820"
     CDS             complement(2030..2731)
                     /locus_tag="PLU11_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPN59859.1"
                     /translation="MISGLTLGAAGSLHCVGMCGPLSLALPTGHLSRAKQFFSLLVYQ
                     FGRIITYSLIGLLFGLAGRRIYISGYQRWFSIGLGILVLALAVIYFVQKNTIHFAFFN
                     RFYNNIQRLIGRILQKNKGPLGFFWLGLANGLLPCGLVYVAIAATLSFSEIIESVSFM
                     AAFGAGTLPAMMMVGMAGSMIKPEARQLIRKAVPVFVTLMGIVLILRGMNLGIPFISP
                     ELPGSPGDAVICHPQ"
     gene            complement(2749..3186)
                     /locus_tag="PLU11_11825"
     CDS             complement(2749..3186)
                     /locus_tag="PLU11_11825"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HPN59860.1"
                     /translation="MNWGNRLVLVFLVFGAGMGYLVYRSMNTDYELVESDYYKQELRY
                     QHVIDEHKSANALSAAVNIEKKEGALLLQFPEEMKNKSLTGELYFYCAYDSKKDKRVS
                     LQSDSNGVQTIPAGTILPGSYTVKIKWSNGEKSYYSEQNLSVL"
     gene            complement(3213..>4378)
                     /gene="ccoG"
                     /locus_tag="PLU11_11830"
     CDS             complement(3213..>4378)
                     /gene="ccoG"
                     /locus_tag="PLU11_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HPN59861.1"
                     /translation="QDFFIFGLTMVTFIIFIVLFTAAFGRLFCGWVCPQTVFMEMLFR
                     KVEFLIEGDGPKQKMLRNSGWTGEKIRKKLTKHLVFFLLSFIIANFFLAYIIGIDELK
                     KIITEPVSQHFVGFMAILLFSAIFYGVYAHFREQACTVVCPYGRLQSVLLDRNSMIVA
                     YDYKRGEPRGKYKKDQPAELALGDCIDCHQCVKVCPTGIDIRNGTQMECVGCTACIDA
                     CDKMMDAVGRPRGLVRYASETGIAEGAKLRYTTRMKLYIVLLIVLTALLTVLLLTRKD
                     VGGTIVRAGGMLFQERGADSISNLYTIKLTNKTMKDINLTLKLEDAPGRIIEAAGENI
                     LVKKEDQGKGSFFIVLPRNYISKRKLSLRVGLYEGDKKITTIKTNFMGPFSLH"
BASE COUNT         1236 a          945 c         1033 g         1164 t
ORIGIN      
        1 ggtcagtttt ctcgataaga ccatgattcc tcatttactg caggaagaag aagtgatatt
       61 cccctatatc cgccagatca gtcatgcgta tgaaagtaag gagtcttatg ccagtttgtt
      121 ggtacgtacg ttgcgtaaac ccgtggaaga cctgatgcat gatgaacata acagtattgg
      181 gaaaacgctt actcaattaa gagaactcac taataactat actgcgcccg agaatgcctg
      241 taccagtcac cggctggcat attctttgat caaagagctt gatgatgacc ttgtgcagca
      301 tatttatctt gaaaacgaca ttcttttccc caaggccatc tctatggaaa aagaattact
      361 ggagaggcac taaaaatttt atacactctt tttttcagat tatcttgctt aattttccgg
      421 caaatggata atcttaacgg acggtcgatg acctattggg ccatcctcgt atttagtttt
      481 tctctcacgt tagctttttt attccgtgac tatcagcttt cggtcagcgg gctgctgctt
      541 tttgtgattc tcacggcttt catcccgggc tggcgatcaa cgctggcagc cggtgtaata
      601 agtatggttg ttatgaccgc tcttgtcatt tattttaaaa aagatgacga tgatattacc
      661 cgggccctcc tctctcaaat ctattccctg tttgtagtgg tgttttccgt agtggtaatt
      721 ttttacctga aaaagatgca gcagaatttt gcccatgaaa aaacgcatat ggcttccctg
      781 ttcgagaatg ctacagaggg gatcctgctg actgataaat ccggcagtat tgtactggta
      841 aatccggctg cagaacggat gtttggttac ggagctgagg aactgattga agaaaaaata
      901 gagaaactga ttccaatgcg tttcctggat cgccatgtag gattgcggga aggattctat
      961 aaacaccctt ccaatcgcac catgggctcc ggccgggatc tttatgcccg ccggaaagac
     1021 ggtagtgaat tccccgtgga agtgagtctg agtcattaca agcaaaagga cgaggatttt
     1081 gtaatcggct ttatcgtgga tatcaccgcc cgtaaagaaa ttgagaaaaa cctgttgcgc
     1141 cagaaaatgg aactggagaa aataagtgat gatatccgga aactgaatac tgacctggaa
     1201 ggtaaagtgg aggaaagaac cattatcctg aaagaagccc tgcagaaact ggaagagtca
     1261 caacaggagc ttagtgaatc cctggataaa gagcggcagc tgaatgagat caaaagtcgc
     1321 tttgtgtcta tggcttccca tgaattcaga acaccgctga gtaccattct ttcttcagcc
     1381 accctggtct cgaaatatcc ttcgggggag gagtttgata aacgggaaaa acacatccgc
     1441 cggattaagg attctgtgaa tcatatgaac gaactgctgg aagactttct gtccctgggt
     1501 aaactggaag aagggaaagt gggagtgacg gtcactgttt tttcagtaaa agattttgtg
     1561 gaggatgtgg tggatgagat gaaggcccat ttaaagagcg gccaggaaat tgtattggat
     1621 tgtatcgggg atggtaattt taagaccgat aagcggatgc tgaaaaatat catgctgaac
     1681 ctgatttcca atgccatcaa gttttcaggg gaagggaaga aggtctggct ttgtaccaat
     1741 acggtaaata gcgtattaaa tatttcggtc cgcgaccagg gacttggcat accggaggaa
     1801 gaccagcccc atttgttcag cacttttttc cgggccaaga atgtaagtaa tattcagggt
     1861 acgggtctgg gacttccgat tgtcaaacgt tatgtaaatc tgttgggtgg cgatatacac
     1921 ctgaacagtg aattggaaaa aggtaccgaa gtggtgatca gtttgccaga atacaaagaa
     1981 ccggattagt atttcccacg gggaaggggg tacagattaa gaaagtgaat tactgcggat
     2041 ggcagatcac ggcatcgccg ggggaaccgg gtaattccgg actgataaag ggaatcccga
     2101 ggttcatgcc ccgcaggatg agcacaatac ccatcagggt aacaaatact ggtacagctt
     2161 tacgaatcag ttgccgggct tccggtttga tcatactacc ggccattccc accatcatca
     2221 tagcaggcag ggtgccggct ccaaaagcag ccataaaact tacactttca attatttcac
     2281 taaaggagag ggtggctgca atagccacat agaccagtcc gcagggcaat agcccattgg
     2341 ccagtcccag ccagaaaaaa cccaacgggc ctttattttt ttggagtatg cgtccaatca
     2401 ggcgttggat attattataa aagcggttga aaaaggcaaa atgtatggtg tttttctgta
     2461 caaaataaat aacggccagg gcgagaacga ggatgcctaa cccgatggag aaccagcgtt
     2521 gatatccgct gatataaatc cggcggccag cgagcccgaa caataatccg atgaggctat
     2581 aggtaatgat tcgtccaaac tggtagacca gcagggagaa aaactgtttt gccctggaca
     2641 ggtgtccggt aggaagggcc aggctaagtg gaccgcacat gcccacacag tgcaggctgc
     2701 cggccgcacc aagtgttaaa cctgatatca tggccgccca tagcatgatt aaagtaccga
     2761 taagttttgc tcggaataat aggacttttc gccattgctc catttaattt tcaccgtata
     2821 agaaccggga aggatagtac cggccggaat ggtttgtacc ccattgctat ctgattgcag
     2881 cgaaacccgt ttgtcttttt tagaatcgta ggcacagtaa aaataaagtt cgccggtcag
     2941 tgatttattt ttcatttcct ccggaaactg cagcaggagg gccccttctt ttttttcaat
     3001 atttacggct gcagagagcg cattggccga cttgtgctca tctataacat gctggtagcg
     3061 taattcttgt ttataataat cggattccac caactcataa tccgtattca tggaacgata
     3121 gaccaggtag cccatgccgg ccccgaatac aagaaaaacg agtactaagc gattgcccca
     3181 gttcatatta attggttttt gtcaggtaac ttttagtgaa gactaaaagg tcccatgaaa
     3241 ttagtcttta ttgtcgtgat tttcttatcc ccttcatata aaccaacccg cagactgagt
     3301 ttgcgcttac tgatatagtt tctgggaagt acaataaaga atgagccctt tccctggtct
     3361 tcctttttta ccagaatatt ttcaccggca gcttcaataa tacgtccggg tgcatcttcc
     3421 agtttcaggg tcaggttgat gtccttcatt gttttgttcg tcagtttgat cgtatacagg
     3481 ttggagatac tatcggcccc tctttcctgg aatagcatac ctccggctct tacaatggtg
     3541 ccacctacat ctttgcgggt cagtaaaaga acagttaaga gggcggtcaa tacgatcagt
     3601 aaaacgatat aaagtttcat gcgggtggtg taacgcagtt tagcaccttc cgcaatacct
     3661 gtttctgaag catatctaac gagtccgcgg ggtcttccca cggcatccat cattttatca
     3721 caggcatcga tacaggcggt acaaccgaca cattccatct gggtgccgtt gcggatatca
     3781 ataccggtag gacaaacttt cacacactgg tgacagtcga tacaatctcc caatgcaagt
     3841 tctgcaggtt ggtccttttt atattttccc cggggttctc cacgtttgta atcataggcg
     3901 acaatcatgg aattgcgatc cagtaatacg ctttgtaatc tgccgtaagg acataccacc
     3961 gtgcaggcct gttcgcggaa atgggcatat acaccataaa aaatcgccga gaacagaagg
     4021 atggccataa agcccacaaa gtgctggctt accggttcag tgatgatttt tttcaattca
     4081 tcgatcccga taatataggc gaggaaaaag ttggcaataa taaaagagag taaaaagaaa
     4141 accaggtgct tagtcagttt cttcctgatc ttctctccgg tccaacccga attccgcagc
     4201 attttttgtt taggtccgtc tccttcgatc aggaattcta ctttacggaa gagcatttcc
     4261 ataaataccg tctggggaca aacccaacca cagaataacc ggccaaatgc ggcggtaaac
     4321 aatacaatga aaatgataaa ggtgaccatc gtaagcccga agatgaaaaa gtcctgtg
//
LOCUS       DAOPMW010000278         4372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23519_length_4372_cov_8.573546, whole genome shotgun sequence.
ACCESSION   DAOPMW010000278 DAOPMW010000000
VERSION     DAOPMW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23519_length_4372_cov_8.573546"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1424)
                     /locus_tag="PLU11_11835"
     CDS             complement(300..1424)
                     /locus_tag="PLU11_11835"
                     /inference="COORDINATES: protein motif:HMM:NF019096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HPN59862.1"
                     /translation="MKKYPYQNTGLKSLKGERWKNIPGFEMYFMVSNFGRIKRLEYEL
                     EYSDGRVYLKPARIIKPVLVKIPNVFMKDHIYFLRTSITLYKQKYHFSIARLVYHCFK
                     SPIKLKDESIVILTKDRNGQNIRVSNLVKASLSQKQKRISDLNRRTPLVVDKEARIRG
                     IAQAKLTNNKRVTQYNLQGKKVKTHPSIAVAAEKIGISHSLISNRARGTEFSAGGFIW
                     RFGDESVIDILPMLKTIAERRKKNKAIFGKKVTQYKMNGRRVAIFPTITDAARETGIK
                     RAEISRVIQKNRYSTGGFFWQEGEGPALIDLSGHEYGEVVRAKNRQRPVQQYSIQGKP
                     LQQFDSIKKAAEVVGVKSTTIIGALTGKQQSAGGYQWKYL"
     gene            complement(1534..>2163)
                     /locus_tag="PLU11_11840"
     CDS             complement(1534..>2163)
                     /locus_tag="PLU11_11840"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPN59863.1"
                     /translation="KRRTKDVDIAVLLDAEEKFYQIKEALVRTGDFEERVNKPIKLIY
                     RGETEVDLLPFGEIENEDRELFLNRHALLVMDMSGFREVYPFVNTVEIESGVSLNVCS
                     LEGLILLKLIAHHDNPSRTKDITDIEHLLKVYFELNSHEIYTDYISVMEMYSTVTNNY
                     LDLVSARVIGRKIGLMLKDYSKTSERIKAILSSRPVPAWLAMLDGMNES"
     assembly_gap    2164..2263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2264..3115)
                     /locus_tag="PLU11_11845"
     CDS             complement(<2264..3115)
                     /locus_tag="PLU11_11845"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HPN59864.1"
                     /translation="MRQVEIRNTAIQRFGDITGINIKIIDNETNTQNKWLADAQLILY
                     TGKQETRFWAEIKNEFRARDLPAMLARAGKNKEAWILICQYIPGPLKEVLRHEGINYL
                     EAAGNCFIRQTGLYFYINDQPVTALRTPKEGKLWKQAGLKFLFGVLQKPEWLNLPYRQ
                     VAELTGVALGNIGPFIDEMKKEGYLKQGMKEGVYIWILENKEALLKKWVELFVVVLRP
                     KLRTGLFRFLNNKEQNAWMNLPDQDFFWGGETGGARLTNYLVPEKYSIYTREDKMKLM
                     KDLKLVPD"
     gene            complement(3240..>4372)
                     /locus_tag="PLU11_11850"
     CDS             complement(3240..>4372)
                     /locus_tag="PLU11_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPN59865.1"
                     /translation="ITEHNAVKYELTENYRSKPEIVQFANSWAETISKRMKSQPGFAV
                     QQTSGEVAITEHAGTKLVVPVVNAILSASLKGTTAVLTRSNEEAMLVNGLLNKHKIPA
                     KLIQSLDGFPLSALYEIRYFSDMVLADPDSPTISPERWQESVRKLEIHARESRYLDLV
                     VRLIRDFEAVNTQRKYKSDWLSYLRESRLEDFVNADSQTVIVSTVHKAKGKEFDNVFV
                     LLSSLDTSKDEDKRVFYVAITRAKSNLQVHYTGTFLKPIASEPVIYQTDNEVYGDPKV
                     IATSLTHKDVFLGFFAGKQYLMENLFSGSTLNFNEGGLCNVRKQELIRYSNAFKLKLE
                     EYQQKGYKPVAARVGFVVYWMDKKTEKEVKLILPEIELERAD"
BASE COUNT         1108 a         1036 c          783 g         1345 t
ORIGIN      
        1 ggagtggaga aaaagcgggt taaaaagaga aatccagtga ataccagtaa gctttacttt
       61 tccgtcaaca attccttcaa tctgtaattg acgtaataca ttggccttct tattgtctta
      121 gttttaaaat atttgcctcc gtcaatttat tcgatattga tttcatgtcg aattcattaa
      181 tcattttcga catgaagttt gtttggtgtc gattcggtga taatcaagaa tgctaaatga
      241 tgaaaaattg tctttttttc gacatgaaac tactgagcaa tggggtttta taaagatcat
      301 caaagatatt tccactggta gccaccagct gactgctgtt tacccgttaa agcaccaatt
      361 atcgttgtac ttttaacccc aaccacttca gcggcttttt ttatactgtc aaattgctgc
      421 aacggctttc cttgtattga atattgctga actggccgct gccggttttt tgccctgaca
      481 acttccccat actcatgacc agataaatca attaaagcag ggccttctcc ttcctgccaa
      541 aaaaatccac cggtactata cctgtttttt tgaattaccc gggaaatttc agcacgttta
      601 ataccggttt cccttgctgc atcagtaatg gttgggaaaa ttgccacacg acgaccgttc
      661 attttgtatt gggttacttt cttaccaaag attgctttgt tttttttcct gcgttcagca
      721 attgtcttaa gcattggcag aatatcaatt actgattcat caccaaaacg ccagataaat
      781 cctccggctg aaaattcagt acctctggcc cggttactga taagactatg gctgattccg
      841 attttttcag cagcaaccgc gatacttggg tgggtcttaa cctttttacc ctgtaggttg
      901 tattgggtta cccgtttgtt attggtcagc tttgcttgcg caataccccg tatcctcgct
      961 tccttatcaa caacaagcgg cgtcctgcga tttaaatcgg atatgcgttt ttgtttctga
     1021 gaaagagaag ctttaaccaa attagaaacc ctgatattct gaccatttct atctttagtg
     1081 agtatcacaa tcgattcatc cttcaattta atcgggctct taaaacaatg ataaactaac
     1141 ctcgctatag agaaatgata tttttgtttg tataaagtga tggaagtacg caaaaaataa
     1201 atatggtcct tcataaacac gtttgggatt ttcaccaata ccggtttaat tattcgcgct
     1261 ggctttaagt acaccctgcc atcactgtat tcaagttcat attccagtct tttaattcgc
     1321 ccgaaatttg aaaccataaa atacatttca aagccgggaa tatttttcca gcgttctcct
     1381 tttaaactct tcagtccggt attttgataa gggtattttt tcatttccta ttcaagataa
     1441 ttaatgcagg ataaacgtgg atatcagcta aagccaccgc ttttgtcatc catcatcgcc
     1501 ccgatcatcc aggcccttac tgtaagggtt tgcttagctt tcattcatgc cgtccagcat
     1561 ggcgagccag gcaggtacag ggcggctgga taatatggct ttgatccgtt cgcttgtttt
     1621 tgaatagtct tttaacatga gtccgatttt tctgcctatt acccgggcag aaacgagatc
     1681 gagataattg tttgttaccg tactatacat ttccataacc gaaatatagt ctgtatagat
     1741 ttcgtgactg ttcagctcaa aataaacctt caataaatgt tcaatgtctg ttatatcctt
     1801 agtgcgtgat ggattatcat gatgagcaat cagtttcagc aatattaaac cctccagtga
     1861 acagacattt aatgaaacgc ccgattcaat ttctactgtg ttgacaaatg gatatacttc
     1921 ccggaatcca ctcatgtcca taaccagtaa agcgtgccgg ttaagaaaca attccctgtc
     1981 ttcattttca atttccccga aaggcagtaa gtcaacttcg gtttctcctc tgtatatcag
     2041 cttaatgggt ttattgacac gctcttcaaa atcacctgtt ctaacaaggg cctctttaat
     2101 ctgataaaat ttttcctctg catccagcag gaccgcaata tcaacatcct ttgttcttcg
     2161 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatccggt accagtttca
     2281 ggtccttcat cagtttcatt ttatcttcac gggtgtaaat gctgtatttt tccggcacca
     2341 ggtaatttgt cagcctggcc cctcccgttt cgccacccca gaaaaaatcc tgatcgggta
     2401 aattcatcca cgcattttgt tccttattat tcaggaaccg gaataatccg gttctgagtt
     2461 ttggccggag taccaccaca aataattcca cccatttctt taataacgct tctttgtttt
     2521 ccagtatcca gatatagact ccttccttca tgccttgttt caaataccct tcctttttca
     2581 tctcgtctat aaacggacca atattgccca gcgccacacc cgtcagctct gccacctgtc
     2641 gataaggaag attcagccat tccggctttt gaagtacacc aaacaagaac ttcaaaccgg
     2701 cctgcttcca tagctttccc tctttgggag tccggagggc agttaccggc tgatcattta
     2761 tataaaaata gagcccggtt tgccggataa aacagttacc cgccgcttcc agatagttga
     2821 tcccttcatg ccggagcacc tctttcagcg gcccgggtat atactggcaa atcagtatcc
     2881 aggcctcttt atttttgcca gcccttgcca gcatggccgg caaatccctg gccctgaatt
     2941 cgttctttat ttccgcccaa aaacgggttt cctgctttcc ggtatacagt atcaattggg
     3001 cgtcagccag ccacttattt tgcgtatttg tctcattatc aattatttta atattgattc
     3061 cggtgatatc cccaaaccgc tgaatggctg tatttcttat ttctacttgc cgcatgttca
     3121 ataaaatatt tcgttcatga atcatgaaca atcaaattta ttgaacatta cgtgaaaatc
     3181 caaacattat tttcccgacg ggagatagta ctgtcttatc atcaacatag gttccttcat
     3241 cagtccgctc gctccaactc aatctccggc aaaatcaact tcacctcctt ctctgtcttc
     3301 ttatccatcc agtaaacaac aaaacccacc ctcgcagcca ccggtttata ccctttctgc
     3361 tgatactcct ccagttttag tttaaaggca ttggaatacc tgattagctc ctgtttgcgg
     3421 acattacaaa gaccgccttc attgaagttg agtgtggaac cactaaacaa attttccatc
     3481 aggtactgct tacccgcaaa gaagccgaga aacacatcct tgtgagttag tgaggtggca
     3541 attacttttg ggtcaccata tacttcgttg tctgtttggt aaatcaccgg ctccgatgca
     3601 attggcttta gaaaagtccc ggtatagtgt acctgcagat ttgatttggc ccgggtgatg
     3661 gctacataaa aaacccgctt atcctcatcc tttgaggtat ccaatgaaga taaaagcaca
     3721 aatacattat caaactcttt gcccttggct ttgtgcaccg tgcttacgat cacggtttgg
     3781 ctatcggcat tgacaaaatc ctccagcctg gattcccgta aataactcag ccagtcactt
     3841 ttgtacttac gttgggtatt aacggcttca aaatcacgga tcaggcgaac caccaggtcc
     3901 aggtaccggc tctcccgggc atgaatctcc aatttgcgta ccgactcctg ccagcgttcc
     3961 ggacttattg tagggctgtc cgggtcagcc aataccatat cggagaaata ccggatctca
     4021 tacaaggcac taagcggaaa accatccaat gactgaatga gctttgcggg tattttatgc
     4081 ttattcagca agccattcac caacatggcc tcctcatttg accgggtaag cacagcggtg
     4141 gtgcctttta aagaggccga taaaatagca tttaccaccg gaacaaccag tttagtcccg
     4201 gcatgctctg tgatggctac ctcgccgctg gtctgttgca cagcaaaccc tggctgcgat
     4261 ttcatccgtt tgctgatggt ctccgcccag ctgttggcaa actgtacaat ctccggtttg
     4321 ctgcggtaat tctctgtcag ctcatatttt acagcattgt gctccgtaat ca
//
LOCUS       DAOPMW010000279         4369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23549_length_4369_cov_7.920260, whole genome shotgun sequence.
ACCESSION   DAOPMW010000279 DAOPMW010000000
VERSION     DAOPMW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23549_length_4369_cov_7.920260"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..613
                     /locus_tag="PLU11_11855"
     CDS             35..613
                     /locus_tag="PLU11_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN59866.1"
                     /translation="MTPERNQNISDSDLIRGCMESDRRMQEELYRRFSPRMYAVCLRY
                     AGNAEEAEDILQEGFIKVFKKLDSFRNEGSFEGWIRRIFVNTAIEHFRRKKYLLPVTE
                     KEENTIEGKYTSVLDELGAKDIMALVQELSPGYRTVFNMYVVEGYTHKEIADQLGISE
                     GTSKSQLSRAKVILQDMVRTHIDRQRGISTKS"
     gene            610..1500
                     /locus_tag="PLU11_11860"
     CDS             610..1500
                     /locus_tag="PLU11_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59867.1"
                     /translation="MSEQIRNTLLNHEVPPPPAAWEHILSELREMEAYKTTGHQLAVA
                     EVNPAPHNWNRILSGLEEISIGQQLANTEVTPPHGTWDKIAHELDTEQQPIKRITPWW
                     RYAAAAAILVTIGVGIMRMVQPGVKGEEMALHQDTLPAIDPGKSDLTKETLDELVATA
                     SAENEDARNDAALEASKKTYAKLEYPDKKIAREVSGFYFNTYSGDAGTRGLTTEETVL
                     SENKPAERYIKLMTPEGNIIRVSKKLGGMVCCLSGEAEDPACTDQLKKWREKMITSSL
                     GHSGDSFLDLLLLINTEEEN"
     gene            1535..1969
                     /locus_tag="PLU11_11865"
     CDS             1535..1969
                     /locus_tag="PLU11_11865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPN59868.1"
                     /translation="MGRNKLVTPAVFTFSCPIPVRITDINYGGHAGNDTILSLIHEAR
                     MQYLGSFGYTEMNLAGSALIMSDVCIEFKKELFYGESLMAFVTAGEISRVGFELFYKL
                     EKNTPAGNITVALARTGMVCFDYTLKKITGVPEEARLKMKGF"
     gene            complement(1966..2700)
                     /locus_tag="PLU11_11870"
     CDS             complement(1966..2700)
                     /locus_tag="PLU11_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPN59869.1"
                     /translation="MRLFGLIGNPLGHSFSEKYFTRKFEELQIHNCRYKLFPLSAITE
                     LPALLDAHPSLEGLNVTIPYKKAVLAYLTDSLIPEGLNACNCIKIRNGKLTGYNTDVL
                     GFGKSLTPLLNSAQQRALVLGNGGATESILYVLKEKGIPFEVVSRSLHKGSTLTYSEL
                     TRDIVQAHTLIINTTPLGTYPHTDNSPDIPYEGITHRHLLYDLVYNPPLTKFLQQGKE
                     KGAQVKNGEEMLVIQAEESWSFWNRD"
     gene            complement(2700..3473)
                     /locus_tag="PLU11_11875"
     CDS             complement(2700..3473)
                     /locus_tag="PLU11_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HPN59870.1"
                     /translation="MNFNLTQIPDRKQKPRQSGLTMVMDKGLSIEEAKNFMSVSYPHV
                     DIVKLGFGTSFVTPNLREKLEVYRSYDIPVYFGGTLFEAFLIRNQFEDYISVCKDFGI
                     SYMEVSDGSITIPHAEKCGYIEKLTKHGTVLSEVGSKDAAHIIPPYKWIELMRAELEA
                     GSSYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPGEQIIWEAPQKAQQLYFIELL
                     GCNANLGNIAPSEVIPLEAMRIGLRGDTFELYLDKKESV"
     gene            complement(3563..>4369)
                     /locus_tag="PLU11_11880"
     CDS             complement(3563..>4369)
                     /locus_tag="PLU11_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN59871.1"
                     /translation="ELAWFNLAAAYQGLKLYEKAIDAYKYAITIEEKFDYAYRNMGDA
                     YIRLRKYKEAIEALEKVNELAKPEDVIFEAIGYCYEKLKNPAQARFHYRKASHLRQDD
                     SKLFYKIASTYFNERQWTACIRQLETAMKIHRNQPEYNLLAGECKLQLGLYREAVQYF
                     STVVRLRPRNANGWEALIRSLYMGGFWEEGIEQAEAALKLTGNKPLFLYYKAALLFAA
                     GKSKEALLWLENALQAAPRLLKKMVELNPALLQNQSLVDLLARYKRNKSI"
BASE COUNT         1198 a         1015 c         1008 g         1148 t
ORIGIN      
        1 atttttgaat agcctgtaat aaaaccacca aatcttgaca cctgaacgca accaaaatat
       61 cagtgacagc gaccttataa gaggatgcat ggaaagtgac cggcgaatgc aggaggaatt
      121 gtaccgccgg ttttcccccc ggatgtatgc tgtctgtctc cgttatgccg gcaatgccga
      181 agaagcggaa gacatcttac aggagggttt tattaaggtc tttaaaaaac tggacagttt
      241 ccggaacgaa ggctcttttg agggctggat ccggaggatt tttgtcaata cggccattga
      301 gcatttccgc cgaaagaaat acctgctgcc cgtaacagaa aaagaagaaa atacaatcga
      361 gggaaaatat acgtccgtgc tggatgaact gggagccaaa gatattatgg cgctggtaca
      421 ggaactgagc cctggttacc gtaccgtttt taacatgtat gtggttgagg gctataccca
      481 caaagagata gccgaccagt taggcatcag cgagggcacc agtaagtcac aattatccag
      541 ggccaaggtc atcctgcaag acatggtcag aacgcatata gaccgtcaaa gaggaataag
      601 tacgaaatca tgagtgagca gatccgtaat acactattga atcacgaagt acccccgcca
      661 ccggctgcct gggagcatat tctttcggag ctccgggaaa tggaagcgta taaaactacc
      721 ggacatcagc ttgccgtggc agaagtgaac ccggctccgc ataactggaa ccggatttta
      781 tcaggccttg aagaaatcag tatcgggcaa caactcgcaa atactgaagt aactccccca
      841 catggaacct gggacaaaat tgcccatgaa cttgatactg aacaacaacc aattaaaaga
      901 ataaccccct ggtggcgcta cgctgccgca gcagctattt tagttaccat tggcgttggc
      961 attatgcgga tggttcagcc cggagttaaa ggtgaagaga tggccttaca ccaggacaca
     1021 cttcctgcta ttgatccggg caaatcggat ctgacaaaag aaacgctgga tgaactggta
     1081 gccacagctt ctgcagagaa tgaggatgcc cgcaatgatg ccgcgctgga agccagtaaa
     1141 aaaacatatg caaaactgga gtaccctgat aaaaaaatag cccgcgaagt atccggcttt
     1201 tattttaata cctatagcgg ggatgcggga acaagaggac ttactaccga ggaaacggta
     1261 ctttctgaaa acaaaccggc tgaacgttat ataaagctta tgaccccgga aggaaatatc
     1321 atccgggttt ccaaaaagct cggcggtatg gtttgctgtc tttccggtga agcagaggat
     1381 ccggcctgta cagatcagct gaaaaaatgg agggaaaaaa tgattacatc atcgcttggg
     1441 cattcaggag acagtttcct ggacttactg ttgctcatca atacggaaga agagaactaa
     1501 ccgataaaaa acagcgaacc ggcactacac aaatatggga aggaataaat tagtaacacc
     1561 cgcggtattt acgttttcct gtccgatccc tgtccggata acagatatta actatggcgg
     1621 acatgccggc aatgacacga tcctgtccct gatccatgag gcgcgtatgc agtaccttgg
     1681 gagtttcggt tatacggaaa tgaatctggc cggttctgca ctgatcatgt ctgacgtgtg
     1741 catagagttt aaaaaagaat tgttttatgg cgaatcctta atggcctttg tgactgctgg
     1801 cgaaatttca cgggttggat ttgaactgtt ttataaactg gaaaaaaaca cccccgccgg
     1861 aaacataacc gtggcccttg ccaggaccgg gatggtctgt ttcgattata ctttaaagaa
     1921 aattacaggt gtaccggaag aagcccgtct gaagatgaag ggcttttaat cccggttcca
     1981 gaatgaccag ctttcttcag cctgaatcac cagcatttct tccccgtttt tgacctgtgc
     2041 ccccttttct tttccctgtt gcaggaattt tgtgagtggc gggttataca ccagatcata
     2101 aagtaaatgc cggtgggtaa tcccctcata aggaatatcc ggggaattgt ctgtatgagg
     2161 atatgtacct aatggtgtgg tattgatgat cagtgtatgt gcttgtacga tatcccgcgt
     2221 gagctcgctg taagtgaggg tagatccttt atgcaggctg cggctcacca cttcaaacgg
     2281 aattcctttt tctttcaata catacagaat cgactcagtt gctccgccat ttcccaatac
     2341 cagcgcccgc tgctgtgcgc tgttcagcaa tggggtcagg cttttcccga accccaatac
     2401 atccgtatta taaccagtca gctttccgtt tctgatctta atgcaattgc aggcatttag
     2461 tccttccggt ataagagagt ccgttaagta agctaaaacg gctttcttat aggggattgt
     2521 gacattcaat ccttcgagtg aaggatgcgc atccagtaat gctggcaatt cagtgatagc
     2581 tgaaaggggg aacaatttgt accggcagtt gtggatttgc agttcctcaa acttccgggt
     2641 aaaatacttt tcagagaaag aatgtcccag tggatttcca atcaggccga atagccgcat
     2701 cagaccgatt cttttttatc aaggtatagt tcgaaggtat cacctcgtaa accgatccgc
     2761 atcgcttcca ggggtattac ttcagaaggt gcaatatttc ccaggttggc attacaaccc
     2821 agtaattcga taaaatagag ctgctgtgct ttctggggtg cttcccagat gatctgctca
     2881 ccgggaatct gtgtcagaat ttcctgcacc aatccttccc gtacttcgcc actaccccgg
     2941 tatataccca cattaccggc ctcccgggct tctgctatta cataagagga tccggcttcg
     3001 agttcagccc gcatcagttc aatccatttg taggggggaa tgatatgggc agcatcttta
     3061 ctgcctactt cgctcagtac agtaccatgc ttggtgagct tttcaatata gccacacttt
     3121 tcggcatgcg ggatggtgat agagccgtca ctgacttcca tatagctgat gccaaaatcc
     3181 ttgcaaacac tgatataatc ttcaaactgg ttacggatca ggaaggcttc aaacaatgtt
     3241 ccgccgaaat aaaccgggat atcgtaggaa cggtacactt ccagcttttc ccgcaggttg
     3301 ggtgtcacga atgaagtgcc aaatcccagt ttaacaatat ccacatgcgg ataagaaaca
     3361 ctcatgaaat ttttggcttc ctcaatactc agccctttat ccattaccat tgtaaggcct
     3421 gattgcctgg gcttttgttt cctgtccggt atttgcgtta aattaaaatt catactctta
     3481 taattaaatg tacctcaata ttaaaacgcg gcaaaaataa ggaagattcg cgggcttctg
     3541 tgtgatttaa tgaaggaacg gatcagattg acttgtttcg cttgtacctg gcaagcagat
     3601 cgaccaatga ctggttctgc aggagggccg gatttaattc caccattttt ttcaataacc
     3661 ggggtgctgc ctgtaatgca ttttccagcc acaaaagggc ctcttttgat ttaccggcgg
     3721 caaacagcag tgctgcttta taatacagga acaggggttt gtttccggtt aacttcaaag
     3781 cggcttccgc ctgttctatt ccttcttccc aaaaaccacc catgtacagg ctgcgtatca
     3841 gggcttccca tccgttggcg ttccgtggac gcaaccggac aacggttgaa aaatactgta
     3901 ctgcttcacg gtaaaggcca agctgcagtt tacattcacc ggccagtaaa ttatactccg
     3961 gttgattacg gtgtattttc atggcggttt ccagctgacg gatacaggcc gtccactgcc
     4021 tttcattaaa ataggtggac gcgattttat aaaacaactt actatcgtcc tggcgcaggt
     4081 gcgaagcctt ccggtaatga aagcgggcct gggccggatt cttcagcttt tcataacaat
     4141 aaccaatcgc ttcaaaaatc acatcttccg gcttggcaag ttcatttact ttctcaagcg
     4201 cttcaatcgc ttctttatat ttccttaagc ggatataggc gtcccccata ttccggtaag
     4261 cgtaatcaaa tttttcttca atcgtgatcg cgtatttata ggcatctatg gctttttcat
     4321 agagctttaa accctggtaa gcagccgcaa ggttaaacca ggccagttc
//
LOCUS       DAOPMW010000280         4367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23557_length_4367_cov_7.716373, whole genome shotgun sequence.
ACCESSION   DAOPMW010000280 DAOPMW010000000
VERSION     DAOPMW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23557_length_4367_cov_7.716373"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLU11_11885"
     CDS             complement(<1..280)
                     /locus_tag="PLU11_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59872.1"
                     /translation="MSQINYLRQVTLFILSAVFAFSATELHQLIRLPQLVAHYHEHKK
                     EDPSLSLIGFLQLHYTANHPMDNDEEDDNQLPFKTTDAIGHVDFSIGIG"
     gene            complement(361..1458)
                     /locus_tag="PLU11_11890"
     CDS             complement(361..1458)
                     /locus_tag="PLU11_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPN59873.1"
                     /translation="MPDLRSIQTEVDAGYLYSQLAKHEENEQVADIFRQMSEIEQGHA
                     EAFLKKINLPAEKMPSPSFRAKTLNRIGKVFGYDYVLGTLMDTEKSLSSSVINLKKKT
                     KMPVTGTETNHVKVLRNIVENSAVSGSTIARFETKHRSVGGNALRAAVLGGNDGLVSN
                     FSLVMGVAGATHGQEGVLLAGLAGLLAGALSMALGEWISVKSSQELYENQMEIEMEEL
                     QTSPENEMKELELIYKAKGIPEQQAKEMAAQLLKDPEHAHELLVKEELGINAEELKGS
                     AFEAALYSFFLFAIGAIIPVFPFMFLSGTNAILLSTAASAVGLFVIGAAITLFTGKSI
                     WYSGFRQVIFGLAAAAITFGIGKLIGVSVAG"
     gene            complement(1488..2234)
                     /locus_tag="PLU11_11895"
     CDS             complement(1488..2234)
                     /locus_tag="PLU11_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59874.1"
                     /translation="MNTVIPTTASIVNKPRKVIRNAVIFTLLMIAGAVITGNSGLDGM
                     NGGYALIVLFGFLAMSSLVTALVYLPRAREFDKLVSQLHPLAHWTYSNEEWNAFIKED
                     LKEMIEVNKATLRLVIIISLVVGAILLLLYRDNLFILIIGGIILMLSLVAFLAPRIRS
                     RILRKGIHESLIGETSAYVGGTFQTWKQLGAHLVGTDIYEEAEIPVLHIIFEFPTLQA
                     SQQEIIRIPVPAGKMEEARKITEILRKQIN"
     gene            complement(2244..>4367)
                     /locus_tag="PLU11_11900"
     CDS             complement(2244..>4367)
                     /locus_tag="PLU11_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN59875.1"
                     /translation="VTRKNLPGTQKCINDLNKLQLNMPGQMANKWSQEKSRKFNDLLK
                     SCDSAYHLTRTNWTNDFKAKDFENALPRLEAFKAEWTAMPETMKEIESSIQLCKMQIA
                     EKKKIYEDFLVTRSKFENGLPIDPKQNATTIEYTASTRFSSNDARQKEMIGFARGMDK
                     RQKDMIANRDRAAQLKKEGQQAETAGQKETALGKYKESVALIPDAVLENRIRVLETEV
                     AAIKTRKAKADQLWNDGLKLADKKKTKKEGLGKMKESLEWWSDADRVKKIRDLEKEVN
                     GSFSNLDIRGTWRHGNSETMTFTPLGEGQYAVIEKGFDNATGTLSMMGENGVIQYTTK
                     SGISGQYLLSFSADGNKATGKWNDNRNESGIRSFTRISKPETPVTVKTPEPAPVKTPE
                     PVTTQTPPTKKKKKSFTDVLNGINKGLGKLDSTISGKKPVPPKTEPSGPVIPPVNNDV
                     GVNPADVATEEKIFDNGNIGGVSGGPSQPTRFTLSKTTYITRIENYHYFNGGKKPGTI
                     ALLHSNGQQYGPWQSYGLIGQGGVQNAYWVAQPKMQLPAGTYTVIDSDPSTWSQNGQS
                     GGAGFTTVWALKSAKQVNQPAKEVTPPPTTQPPVQTNDGTVSQGVIYGTVFKTREESG
                     KTNPYDLEGVPLPNTTVTIKYVQNGQTITKKATTDGSGKFRFTGLPLNVTISINSRNT
                     TVNRLLTSSGPQYVQFGWDGEIEIK"
BASE COUNT         1020 a         1019 c          957 g         1371 t
ORIGIN      
        1 ggccaatacc aattgaaaaa tcaacatgac caatggcatc tgttgttttg aatggaagct
       61 ggttgtcatc ttcttcatca ttatccattg gatgattggc cgtataatga agctgtaaaa
      121 atccaatcaa tgacagagaa gggtcttcct ttttatgctc atgatagtgt gccaccagtt
      181 gtggtagccg gatcaactga tgcagttcag ttgcactgaa ggcgaaaaca gcggataata
      241 taaaaagggt tacctggcgc aaataattga tttgcgacaa acttaattgt attcactgac
      301 ttaagaaatg actttgtccc tggttgggtg ctgattaagc taaaaacctg ttaattaacc
      361 tcaaccggca acggataccc caatcagttt gccgatacca aatgtgatgg cagcggctgc
      421 gagaccgaat atcacctggc ggaaacctga ataccaaata cttttcccgg taaagagggt
      481 aatcgcagca ccgatgacga ataaaccaac tgcactggcg gctgtactta ataaaatggc
      541 attggtgccc gataaaaaca taaaagggaa taccggtatg atggccccga tcgcaaaaag
      601 aaagaaagaa tacagggcgg cttcaaaagc ggaacctttt aattcttccg cattaatacc
      661 cagttcttct ttgaccagta attcatgtgc atgttcggga tcttttaata actgagcggc
      721 catttctttt gcctgctgct cagggatgcc tttggcttta taaatcagtt ccagttcttt
      781 catttcattc tccggactgg tctgcaattc ttccatttca atttccatct ggttttcata
      841 tagttcctgg gaactcttca ccgagatcca ttctccaaga gccatggaaa gtgctccggc
      901 cagtaatccg gcaagtcctg ccagcaacac gccttcctgt ccatgcgtgg caccggccac
      961 gcccatcact agactgaagt tggatacaag cccatcatta cctcctaata ctgccgcacg
     1021 caaagcattg ccgcccactg aacggtgttt ggtttcgaac cgcgcaatgg tactgccgga
     1081 aacagcggag ttttcaacaa tattcctgag tactttgaca tggttggtct cggtacccgt
     1141 tacaggcatt tttgttttct tcttcaggtt gatgaccgaa ctggaaagac ttttctccgt
     1201 atccatcagg gtacccaata cataatcata gccgaacact tttcctatcc ggttcagtgt
     1261 ttttgcgcgg aacgagggtg acggcatttt ttctgccggc aggtttattt ttttaagaaa
     1321 ggcttcagca tggccttgct caatttcact catttgcctg aaaatatcag ctacctgttc
     1381 attttcctca tgtttggcca gctgactata aagataaccg gcatccactt ctgtctgaat
     1441 acttctgaga tcgggcattt gaggtatgtt taattatcaa agttatttta attaatctgc
     1501 tttcttagta tttcagttat tttacgcgct tcctccattt tgccggctgg taccgggata
     1561 cggattattt cctgttggga agcctgcagg gtaggaaact caaaaatgat atgcagtact
     1621 ggtatttcag cctcttcata gatgtcggta cctaccaggt gagcgcccaa ttgtttccag
     1681 gtctggaaag taccacccac ataagccgat gtttcgccga tcagtgattc atgtattcct
     1741 ttacggagga tacgcgaacg tatacgggga gccagaaaag cgacaaggga aagcatcagg
     1801 ataattccgc cgataatgag aataaacagg ttgtcccggt atagcagcag caggatcgca
     1861 ccaactacca gtgatatgat gatgaccagc cgcagggtgg ctttattcac ttcaatcatt
     1921 tctttaagat cttccttgat aaaagcattc cattcttcgt ttgaataggt ccaatgtgcc
     1981 agcggatgca attgactgac cagtttgtca aattcccttg cccttggcag atagacaagg
     2041 gccgtgacaa gggaggacat ggccagaaat ccaaatagta caatcaatgc atagccgcca
     2101 ttcatgccat ccagaccgct gttgccggtt ataacagccc cggcaatcat aagaagagta
     2161 aatatcacag cattccggat tactttccgg ggtttgttca caatggaggc tgttgttggt
     2221 attaccgtat tcatttttta agattattta atttctattt ccccatccca gccaaattgt
     2281 acatactgtg gtccggaact ggttaacagc cggttgaccg ttgtgttcct gctgtttatg
     2341 gaaattgtaa cattcagggg caacccggta aagcggaatt tcccacttcc atctgtagtt
     2401 gcttttttgg ttatcgtctg gccgttttgt acatacttta tagtaacagt ggtattaggc
     2461 agaggtaccc cttccaggtc atatgggttt gttttcccag attcctcccg ggttttaaat
     2521 acggtgccat atataacccc ctggctaact gtcccatcat ttgtttgaac tggtggctgt
     2581 gtggttggag ggggagtgac ttctttcgct ggctgattaa cctgttttgc acttttcaat
     2641 gcccatacgg tggtaaagcc tgctccgcca gactggccat tctgtgacca ggtggaaggg
     2701 tcgctatcaa ttaccgtata ggtgcctgct ggcagctgca ttttcggttg tgctacccag
     2761 taagcatttt gcacacctcc ctggccaata aggccatagg actgccaggg gccgtattgc
     2821 tgaccattgc tgtgcagcag ggcaatcgtt cctggttttt tacccccatt gaaatagtga
     2881 tagttttcaa tccgggtgat atacgttgtt tttgacaatg taaagcgggt cggctgcgat
     2941 ggccctccgg atactccgcc aatattacca ttgtcaaaaa tcttttcttc tgtcgcgaca
     3001 tcagccggat tgacccccac atcattatta accggtggaa taaccggtcc tgaaggttca
     3061 gtctttggag ggacaggttt cttaccgctg atggtgctgt ccagtttacc caatcctttg
     3121 tttattccat tcaatacatc agtgaagctc ttctttttct tcttggttgg cggtgtttgt
     3181 gtggttactg gttcgggtgt ttttacagga gctggttctg gtgttttaac ggttaccggg
     3241 gtttcaggct tactgatccg tgtaaaactc cttataccac tttcattgcg gttgtcattc
     3301 cattttccgg tagccttgtt gccatcggct gaaaaactga gtaaatactg accggagatg
     3361 ccggattttg ttgtgtattg gatgactccg ttttcaccca tcattgacag cgtaccggtt
     3421 gcattatcga atcctttttc aataacagcg tattgtcctt cacccagtgg agtaaaggtc
     3481 atagtttccg aattaccatg ccgccaggta ccgcggatat caaggttgct gaacgagccg
     3541 ttcacttctt tttcaagatc cctgattttt ttcacccgat cggcatcact ccaccattca
     3601 aggctttctt tcattttacc aagcccttct ttttttgttt ttttcttatc ggctaatttc
     3661 aggccatcat tccataattg atctgctttg gccttcctgg tcttgattgc tgcgacttcg
     3721 gtttccagta cacggatacg attttccaat acggcgtcgg ggatcaaagc cacactttcc
     3781 ttgtatttgc caagagccgt ttctttttgt cctgcagttt ctgcctgctg gccttccttt
     3841 ttaagttgag ccgctctgtc ccggttggct atcatatcct tctgccgttt atccataccc
     3901 cgtgcaaatc cgatcatttc tttttgccgg gcatcattac tgctgaaccg ggtgctggcc
     3961 gtgtattcaa tcgtggtggc attttgttta ggatcgatgg ggagcccgtt ttcaaatttt
     4021 gatctggtga ccaggaagtc ctcatagatt tttttctttt cagcaatctg cattttacac
     4081 aactggatcg aactttctat ttctttcatc gtttccggca tggcggtcca ttctgctttg
     4141 aatgcttcca gacggggtaa tgcattttca aaatcctttg ctttaaaatc attggtccag
     4201 tttgtcctgg taaggtggta tgccgaatca caacttttga gcagatcatt gaatttccgg
     4261 ctcttctcct gtgaccattt attggccatc tggccaggca tattcagctg gagtttattc
     4321 aggtcattta tacatttttg tgtaccgggc agatttttcc gggttac
//
LOCUS       DAOPMW010000281         4367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23558_length_4367_cov_6.807978, whole genome shotgun sequence.
ACCESSION   DAOPMW010000281 DAOPMW010000000
VERSION     DAOPMW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23558_length_4367_cov_6.807978"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..728)
                     /locus_tag="PLU11_11905"
     CDS             complement(27..728)
                     /locus_tag="PLU11_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPN59876.1"
                     /translation="MTDAIIALISLIALEVILGIDNIIFISILADRLPEQQRNKLRLW
                     GIGLAMVMRLLLLAVISWILKLDKTLFTLFDKDFSGKGLILIVGGLFLIYKATKEIYH
                     KTEMEDEKPGAGQKATFSRLLGEIILLDLVFSVDSIITAVGMVQDLWVMYTAVVVTVI
                     IMLIASRPISQFIHKHPSFKILALCFLLLIGVSLLAEGFSFEIPKGYIYFSMAFAFLV
                     DIIQMKTVGRKNASR"
     gene            complement(972..2129)
                     /locus_tag="PLU11_11910"
     CDS             complement(972..2129)
                     /locus_tag="PLU11_11910"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPN59877.1"
                     /translation="MPAEWEQQERVYLSWFGRERRDSVSCRVVQALIPHVAITINLQQ
                     ESLLAAVKNKLRTYSIDPEQIQFVIDSSADFWTRDPFLFIQKKNKQLSVVQFRYTMYG
                     AYPDLLGEPPPRDILLVGEYGERLAARLQLPLIKSDYVFEAGGIESNGKGTIMLIREM
                     ALQRNPGKTIEAIELELKRVTGARKIIWLKEGLAEDRIYKNYGPFYKNYFGGGANMHI
                     DELCRFTDAHTVILPYITEADARKSPVDSLNFAMLEDNLAILRSATTADGKKLRIFRF
                     PMPEAETLKYVQVIDPSNQFEVKEWGFKPGDTIYRVPAASYMNYFVSNKVVLIPKYWM
                     PGMPESQQQKDEMARQLFVQLFPGRTIIPIFALSINRGGGGIHCMTHEQPR"
     gene            complement(2423..3052)
                     /locus_tag="PLU11_11915"
     CDS             complement(2423..3052)
                     /locus_tag="PLU11_11915"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN59878.1"
                     /translation="MSSPSRRQFIRSTSIGTGLLGVLPLSGFASEPPADDRPNVIGPR
                     EGFSPQVGTVLSMMTWMRGAVLSSVRNLTMEQLDYLHDDKSNTIGSMLFHLAATERFY
                     QIHTFEGKKWGDWAKADNDRFGIAMELGEEARKVIKGHDLKFYLDTLTEVRDHTIAEF
                     KKRDDQWLMSVDEKWFWGPTNNYCKWFHVCEHESNHNGQMKWIRGRVRK"
     gene            complement(3156..3926)
                     /locus_tag="PLU11_11920"
     CDS             complement(3156..3926)
                     /locus_tag="PLU11_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN59879.1"
                     /translation="MNYFDPISAVTRYAKGRPDFHRNTVGKIQAFLFPGQRVGQALDI
                     ACGTGLSTKALLDIADEVYGTDISRAMLDQAHAPDRIKYSLAPAEQQPFPDHFFDLIT
                     VSSGVHWFDIDRFLAEARRLLKSRAWLVLYENHFIAEMEGHDGFRDWFTDLYLKKFPS
                     PPRNNEYNWSAENLQPRGLAFKFEDRFRNRVEMSKEQLILYFTTQSNIISAVEMGDTT
                     YPQVESWLTEQLSPFFEQGNDKRVIYYGNWIKYIQRTN"
     gene            complement(4031..4303)
                     /locus_tag="PLU11_11925"
     CDS             complement(4031..4303)
                     /locus_tag="PLU11_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HPN59880.1"
                     /translation="MPKYVIEREIPGAGKLTPEQLKGISQTSCGVLSNLGPQIQWLHS
                     YVTGDKIYCVYIAPNEEMVREHAKQGGFPANAVNEVMTVIDPITAE"
BASE COUNT         1127 a         1040 c         1027 g         1173 t
ORIGIN      
        1 gttgactaca gactcccgac tcgctactac cgacttgcat tttttctccc taccgtcttc
       61 atctgaataa tatcgaccaa aaaggcaaaa gccatggaga agtagatata tcctttcggt
      121 atttcaaaac tgaaaccttc ggccagcagg gacaccccga tgaggagaag gaagcatagg
      181 gccagtattt tgaaggaagg atgtttgtga atgaactgac tgatcggccg tgaggcgatg
      241 agcatgatga tcacggtcac cacaacagcc gtatacatca cccaaagatc ctgcaccata
      301 cccacggcgg tgatgatgga atccacggag aagaccaggt ccagcaggat gatctctccg
      361 aggaggcggg agaaggttgc tttttgtccg gccccgggct tttcatcttc catttccgtc
      421 ttatggtaga tctctttcgt ggctttatag atcaggaaga gtcctcccac aatcagtatc
      481 aggcccttgc ctgaaaagtc tttatcaaag agggtaaaca gggtcttgtc cagtttcagt
      541 atccaggaga tcacggccag caggagcagg cgcatgacca tggccagtcc gatcccccat
      601 aaccgcaatt tattccgttg ctgctcgggc agcctatccg ccagtatgga gataaaaata
      661 atattgtcga tcccgaggat gacttccagg gcgataaggg aaatgagggc aatgatggcg
      721 tcagtcataa actactatta caattctgag ggaaattagt tgaaactgtt aagggtgaaa
      781 acaacgataa agtacgaagt acgaggtacg aagtacggaa tacaaagtac aaagtacgag
      841 cggtatttcg taaagcgtgg ggcaagaagg gaataccgga tgccgtaaaa gtgatagaat
      901 tccttgcttt tgcttgaatt tactttggag gccctgacac cttacagcag acacctagca
      961 acgagcactt tctacctggg ctgttcatgt gtcatgcaat gaatgcctcc tccaccacgg
     1021 tttatgctta gggcaaagat gggtataatt gttcttcctg gaaaaagctg tacaaagagc
     1081 tggcgggcca tttcatcttt ttgctgctgg ctctcaggca tacccggcat ccagtatttg
     1141 gggatcagga caactttatt actgacaaag taattcatat aactggcagc cggtacccgg
     1201 taaattgtat cacccggttt gaatccccac tcttttactt cgaactgatt agaggggtcg
     1261 ataacctgta catatttcaa tgtttccgct tcaggcatcg ggaagcggaa tatccgtaat
     1321 tttttaccat cggcggtggt ggcagaccgt agtatggcaa gattatcctc cagcatggca
     1381 aaattgaggc tgtccaccgg acttttcctg gcgtctgctt cagttatata aggaagaata
     1441 accgtgtgag catccgtaaa ccgacagagt tcatcaatat gcatattggc cccgccgccg
     1501 aaataattct tataaaaagg tccgtaattt ttatagatcc ggtcttctgc caaaccctct
     1561 ttcaaccaga tgatcttacg ggcaccggtc actcttttca gttccagttc aatcgcttca
     1621 atggttttac ccggattccg ttgcagggcc atttcccgaa tcagcataat ggttcccttt
     1681 ccgtttgatt caataccacc ggcttcaaac acataatcac tttttatcaa tggcagttgc
     1741 aatctggcgg ccagtctttc tccatattcc ccgaccagca gtatatcccg gggtggcggt
     1801 tcccctaaca gatcgggata agccccatac atagtatacc tgaactgaac aaccgataat
     1861 tgcttattct ttttttgaat aaaaaggaaa ggatccctgg tccagaaatc ggcgctggaa
     1921 tctatgacaa actgtatttg ttccggatct atcgaatatg tcctcagctt attttttact
     1981 gctgcaagaa gtgattcctg ctgtagatta atagtaatag ccacatgagg aatcaatgcc
     2041 tgcacgaccc ggcaactgac gctgtccctt ctttcccggc cgaaccagct cagatacacc
     2101 cgttcctgtt gttcccactc ggcaggcatt ctggcgggct gagctattaa cagttgagct
     2161 gataaggcaa ggatgaacgc gaatgtgaag cggcgaaaat ggttatacat ttgagcaatt
     2221 gtaatgtaaa aaaggtcaag gcaaacaaga tcaatgctat tcatctaatg acttcagact
     2281 ggttcggcgt agtaaatgat tcttattcca tatccaggtt tggcacccga ggctattgct
     2341 atgtcccacc tccacttctt atcctccgaa accttggtgt aggaagttcg ttatggtcgg
     2401 caggcaggct actaactcct gactattttc tcacccttcc cctgatccac ttcatctgcc
     2461 cgttatgatt cgattcatgc tcgcagacat ggaaccattt gcagtaattg ttggtggggc
     2521 cccagaacca tttctcatcc acgctcatga gccactggtc atcgcgcttc ttaaattccg
     2581 caatggtatg atcccggact tctgttaatg tatccaggta aaatttcagg tcatgtcctt
     2641 tgatgacttt gcgggcctcc tctcctagtt ccatggcgat gccgaaacgg tcgttgtcgg
     2701 ccttggccca gtcgccccat ttctttcctt cgaaagtatg aatctggtaa aaacgttcgg
     2761 tggccgcaag gtggaagagc atgctgccga tggtattgga tttatcatcg tgtaaataat
     2821 caagttgctc catggtcagg ttacggacgg aactcagtac ggcgccgcgc atccaggtca
     2881 tcatagaaag tactgtgcct acctgcggac tgaatccttc gcggggaccg attacattgg
     2941 gtcggtcgtc ggcaggtggt tcgctggcaa aaccagataa aggaagtact ccaagaagac
     3001 cggtgccgat agaggtggac cggataaact gacggcggga aggtgaagac atggttatag
     3061 gtttgaggtg attaatgttt ctatcaggaa aatacaaaaa gtctaaaatc cgggcaacaa
     3121 atgatctata attaactgtt aacagatcag catggtcaat ttgtccgctg tatatacttg
     3181 atccagttcc cataataaat aactctctta tcattcccct gttcaaagaa aggtgaaagt
     3241 tgttcagtca gccagctttc cacttgtggg taagtggtat cccccatttc aacggctgag
     3301 atgatattgc tttgcgtggt gaaatacagg atcagctgtt cttttgacat ctccacccgg
     3361 ttcctgaacc ggtcttcaaa tttaaaggcc aaccccctgg gttgtaaatt ttcagctgac
     3421 caattatact cattgtttcg cggcggagaa ggaaacttct tcaggtacag atccgtgaac
     3481 caatccctga acccgtcatg cccttccatc tcagcaataa aatgattctc ataaagtacc
     3541 agccaggcgc ggcttttcag cagccggcgg gcttccgcca ggaaacgatc aatatcaaac
     3601 caatgtacac cggagctaac cgtgatcagg tcaaaaaaat gatcggggaa tggctgttgc
     3661 tctgcgggag caagggaata ctttattctg tcaggagcat gggcctgatc tagcatggcc
     3721 cgggaaatat ccgtcccgta tacttcatcc gcaatatcca gcaatgcttt ggtggagagc
     3781 ccggtaccgc aggcaatatc cagggcctgt cctacccgtt gtccgggaaa caggaatgcc
     3841 tgaatcttgc cgactgtgtt ccggtggaaa tcgggacgcc cttttgcata gcgggtgacg
     3901 gctgatatgg ggtcgaagta attcattggc tatgattact gatcaagagg aaagataatg
     3961 cacttttaaa ataagatgtg ggtaaaccca cttcctaatt ccccattcct aatttctgat
     4021 tttaatttgt ttactcagcc gtaatgggat cgataacagt catcacttca ttgacggcat
     4081 tggccgggaa gccgccctgt ttggcatgct cacggaccat ttcctcattg ggagcaatgt
     4141 acacgcagta gatcttatca ccggtgacat agctatgcag ccattggatc tgggggccca
     4201 ggttgctgag cacgccgcag gaggtttggg agatcccttt taactgttca ggggttaact
     4261 tcccggctcc gggaatttcg cgttcgatta catacttagg cataagtggt tgggttttgg
     4321 tttcctactc atatggcttt tcggttacgc cccgttttta aagtagt
//
LOCUS       DAOPMW010000282         4345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_23739_length_4345_cov_6.854079, whole genome shotgun sequence.
ACCESSION   DAOPMW010000282 DAOPMW010000000
VERSION     DAOPMW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23739_length_4345_cov_6.854079"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLU11_11930"
     CDS             complement(<1..681)
                     /locus_tag="PLU11_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPN59881.1"
                     /translation="MCGIVGYTGPREAYPIIIKGLKRLEYRGYDSTGVALQNGGLNVY
                     KKKGKVAELEDSIIGKNLHSHIGIGHTRWATHGEPSDRNAHPHQSSSGQLAMIHNGII
                     ENYNQLKQELINKGYTFSSDTDTEVLLNFIEEIKKNNDCSLEEAVRVALKRVTGAYVI
                     LLMSADDPETIIAARKGSPLVIGVGKGEHFLGSDASPMLEYTKEVVYVNDYELAIVKP
                     DELVLKNLG"
     gene            complement(796..2187)
                     /gene="mgtE"
                     /locus_tag="PLU11_11935"
     CDS             complement(796..2187)
                     /gene="mgtE"
                     /locus_tag="PLU11_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPN59882.1"
                     /translation="MSLELEQPTLLEQFESVIQTEDRLMIRDFLDDQNISDVAFLVHE
                     FPDFQSQIVANMSVHRAASVFKILDLSIQKSVIHDLPANTTANLLNELPADDRTDFLE
                     ELPSNVVRELIKLLDPDERKVTLSLLGYPENSIGRLMTPDYVYVYPWNTIEEVFSTIR
                     KFGKDSETINVIYVINDKGELLDDIRIREFILNPPDKKVSELMDERFIALFPDNDQET
                     AAEVFKMNNRVALPVVSKSNKLLGIVTIDDILWVTQEEFSEDMQKIGGTAALNEPYLE
                     IPILKLFKKRVVWLVVLFLGELLTATAMAFFEDEIKKAVVLALFVPLIISSGGNSGSQ
                     ASTLIIQAMAIGEITIKDWWRVMRREIISGLLLGAVLGLIGFLRIYAWHSFDPALYGI
                     YWWQIGVTVSVTLLGVVLWGTLSGSMLPLLLKRLGADPAVSSAPFVATLVDVTGLIIY
                     FSVAYAMLSGSLL"
     gene            2303..2686
                     /locus_tag="PLU11_11940"
     CDS             2303..2686
                     /locus_tag="PLU11_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPN59883.1"
                     /translation="MILPFLFIAPTETRGRGVYTSEDIEEGVMVEVSPVVVMSMEERK
                     LLDQTLLHDYIFEWGENKDHCCMALGYVPVYNHSYKSNCEYGMDYEQQLISITTVRAI
                     KAGEELLVNYNGDWDDATALWFDVK"
     gene            complement(2736..3233)
                     /locus_tag="PLU11_11945"
     CDS             complement(2736..3233)
                     /locus_tag="PLU11_11945"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HPN59884.1"
                     /translation="MKLMKLTALPLFMVASLFMISSCESDAELKRTSDYSKTGIAMTG
                     AQETPAVTSAALGTLDVSYTRSSKILTYTFNWSGLTGPVTAAHIHGLAPTGFAAGVVQ
                     TFSTSAITRCATVSNTSCGSYKGSLLVDEVVVKEQDLLNGVYYVNLHTAAFPGGEIRG
                     QIRFQ"
     gene            complement(3320..>4345)
                     /locus_tag="PLU11_11950"
     CDS             complement(3320..>4345)
                     /locus_tag="PLU11_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN59885.1"
                     /translation="AELYDALLNELFDYLAPVQTNGNLLAIMDCLACHAHNAIQYHYK
                     KPVLHTGDEWIVKEGRHPVIERNLPVGETYISNDVELNKNNQQVIILTGPNMSGKSAL
                     LRQTALITLMAHTGSFVPAAEARLPLTDKIFTRVGASDNLSGGESTFMVEMNETASII
                     NNLTSRSLILLDEIGRGTSTYDGISIAWSIAEYLHNSPHQPITFFATHYHELNELEEK
                     CPRIKNFHVTNKEVGNKIIFLRKLARGGSTHSFGIHVAKMAGMPPALLDRANEILGQL
                     ETKHVQDETGEALKKISTPKMQLSIFDAHSETFDEIRRMLEETDINRLTPVEALLKLQ
                     EIKNRLK"
BASE COUNT         1130 a          993 c         1039 g         1183 t
ORIGIN      
        1 cccgaggttt tttaaaacca gttcatccgg ttttacgatc gccagttcat aatcatttac
       61 ataaaccact tctttggtgt actcgagcat cgggctggca tcggaaccca ggaaatgttc
      121 tccttttccc acacctataa ccaacggact tccttttcgt gctgcaatta tagtttccgg
      181 atcatcagca gacatcagca gtatcacata agccccggta acccgtttca aagccacccg
      241 tacggcttct tccaatgaac aatcattgtt ttttttaatt tcttcaataa agttgaggag
      301 tacttctgta tcagtatcgc tggaaaatgt atagcctttg ttgatcagtt cctgtttgag
      361 ctgattataa ttttcaatga tcccgttatg gatcatggcc agctgaccgg acgaagattg
      421 atgggggtgc gcattccggt cactcggttc gccatgggtg gcccagcggg tatgaccgat
      481 tccaatatga gaatgcagat tttttcctat gatactatct tccagttcgg ctaccttacc
      541 ttttttctta taaacattga gtccgccgtt ttgaagggct acgccggtac tgtcgtaacc
      601 gcggtactcc agtcttttca gtcctttgat aattatggga tatgcttccc tgggtccggt
      661 atagccaacg attccacaca taaagtttga tggttttttg cgtcaatagt atcgatttct
      721 aaacatgcaa acgtttgact gcctgcataa gtgtaaaggt gcgaatttaa ttgtagacgt
      781 caaaaactta acgtattaaa gaagactccc gcttaacatg gcataggcca ccgaaaagta
      841 aataatcagt ccggttacat ccaccagcgt ggccacaaag ggggcagaac tgacggcagg
      901 gtcggcgcca agtcttttaa gcaggagggg gagcatacta ccggaaaggg tgccccagag
      961 aactacaccg aggagtgtta cggaaacggt aaccccgatc tgccaccagt aaattccata
     1021 gagtgccggg tcaaaagaat gccaggcata gatccgcagg aaaccgatga ggcccagtac
     1081 ggcgcccagg agtaatccgg aaatgatttc ccgccgcata acacgccacc agtctttgat
     1141 cgtgatttca ccaatagcca tagcctgtat aatcagggtg gaggcttgag aaccgctgtt
     1201 acccccgctg gaaataatca ggggaacaaa taaggccagt acaacggctt tttttatttc
     1261 atcctcaaag aaagccatgg ccgtggcggt cagcagctct cccaaaaaca gcaccacgag
     1321 ccatacgacc ctttttttga agagtttcag aatcggaatc tccaggtagg gttcattcaa
     1381 cgcggcggta ccccctattt tctgcatatc ctcgctaaac tcttcctggg tcacccaaag
     1441 gatatcatcg atggtaacga tgccaaggag tttattgctt ttactgacca ccggtaaagc
     1501 tacccggtta ttcattttaa acacttcagc cgccgtttcc tggtcattat caggaaacag
     1561 ggcaataaaa cgttcatcca tgagttccga tactttttta tccggcggat tcaggatgaa
     1621 ttcgcggatg cggatatcat ccagcaattc tcccttatca ttgattacat agattacatt
     1681 gatggtctca ctgtctttgc cgaatttccg gatcgtgctg aaaacctctt caatcgtatt
     1741 ccaggggtat acataaacat aatcgggcgt catcagccgc ccgatactgt tttcaggata
     1801 gcccagcagg gaaagagtga ctttccgttc gtcggggtcg agtaatttga tcagttcgcg
     1861 tactacatta ctgggcagtt cttccaggaa atcggtacgg tcatccgcgg gcagttcatt
     1921 cagcaggttg gccgtggtgt tggccggcag gtcatggatc accgatttct gaatggagag
     1981 atccagtatt ttaaatacac tggctgcccg gtgtaccgac atattggcca cgatctggct
     2041 ctggaaatct ggaaattcat gtaccaggaa ggcgacatca ctgatattct ggtcatccag
     2101 gaaatcacgg atcatcaggc ggtcttccgt ctgtatgacc gattcaaact gttccagtaa
     2161 agtgggttgt tccagctcca gcgacatggg ccaaaaatac gaaggggggc gcatacctcc
     2221 ctgtcaaatc tgacaggaaa attacccgtt tgttgaggtt tgaatattac cgtacttcgt
     2281 atgcattaaa gcaggatgat ttatgatatt gcctttttta tttattgccc cgacggaaac
     2341 ccgtggcagg ggtgtataca cttcggagga tattgaagaa ggagtaatgg tggaagtgtc
     2401 gccagtagtg gttatgagta tggaagaacg aaaactgctg gatcagaccc tgctgcatga
     2461 ttatattttt gaatgggggg aaaacaagga tcactgttgt atggcattgg gatatgttcc
     2521 ggtgtataat cattcctaca aaagtaattg tgagtacgga atggattatg agcaacaact
     2581 gatcagtatt actacggtac gggccataaa agcaggggag gaactgctgg tcaattataa
     2641 tggcgactgg gatgatgcta ctgcactctg gtttgatgtg aaataaaaaa accggtccgt
     2701 aactgcggac cggtttttta aatatagtta atcggttact gaaaacggat ctgtccgcgg
     2761 atttcgccac cagggaatgc agccgtatgc agatttacat aatacacccc attcaacagg
     2821 tcctgttctt ttaccactac ttcatctacc aacagtgatc ccttataaga accacaggaa
     2881 gtattgctga ctgtagcaca gcgggtgata gcggaagtag agaaagtctg aacaacccct
     2941 gcggcaaaac cagttggggc caatccgtga atatgggctg cggttacagg tccggttaaa
     3001 ccagaccaat taaatgtgta agtaagaatc tttgagctcc gggtgtagga tacatccaat
     3061 gtacccagcg cagcagaggt aaccgcagga gtttcctggg cgccggtcat tgcaattccg
     3121 gttttggaat aatctgaagt tcttttcagt tctgcgtccg attcacagga agaaatcata
     3181 aacagtgaag cgaccatgaa caggggtaaa gcagttagtt tcatcaattt catgattaca
     3241 ttatttgggg taaaaataag gatttttttg aatgggcaat acttttacaa aataagctaa
     3301 aaccctgtgg gtaaactgtt tatttcaggc gattttttat ttcctgcaac ttcaataaag
     3361 cttcaaccgg agtcaggcgg tttatatccg tttcttccag catccggcgg atttcgtcga
     3421 aggtttcact gtgggcatcg aaaatggata actgcatttt cggggtactg atcttcttca
     3481 gtgcttcccc ggtctcatcc tgtacatgtt tggtttccaa ttgcccaagg atctcattgg
     3541 cccggtcgag cagggcaggg ggcatcccgg ccattttagc cacatggatc ccgaagctgt
     3601 gggtgctgcc accccgggcc agtttcctca gaaaaatgat tttattcccg acttctttat
     3661 tggtgacatg gaagtttttg atccggggac atttttcttc cagttcattc agttcatgat
     3721 agtgcgtggc aaaaaacgta atgggctgat ggggggagtt atgcagatat tcagcaatac
     3781 tccaggcaat ggaaattcca tcataagtcg aagtgccccg gccgatctca tccagcagga
     3841 tcaggctccg ggaagtgagg ttgttaatga tagaagccgt ctcattcatt tccaccataa
     3901 aagtggattc gcctcccgac aggttatcgg aggcacctac cctggtaaag attttatcag
     3961 tcaggggtaa acgggcttct gccgccggta caaaactgcc ggtatgcgcc atcagggtga
     4021 tcagggcggt ctgccggagt aaggccgact taccactcat attaggaccc gtcaggatga
     4081 tgacctgctg gttgttttta ttcagttcca catcattgct gatgtaagtt tcgcccaccg
     4141 gcagattacg ttctatcacc gggtgccgtc cctccttcac gatccattca tcaccggtat
     4201 gcagtaccgg ctttttatag tggtattgga tggcattgtg tgcatgacag gccaggcaat
     4261 ccataatggc cagcaggttg ccattggttt gaaccggtgc caggtaatca aacaattcat
     4321 tgagcagggc atcatacaat tcggc
//
LOCUS       DAOPMW010000283         4294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24155_length_4294_cov_7.012503, whole genome shotgun sequence.
ACCESSION   DAOPMW010000283 DAOPMW010000000
VERSION     DAOPMW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24155_length_4294_cov_7.012503"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLU11_11955"
     CDS             complement(<1..463)
                     /locus_tag="PLU11_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59886.1"
                     /translation="MLPLPYLRQGGFTILAGLLLLSLKSTAQLPEDLEKMGKEAGGYQ
                     FHLKFRGTTTTISDEKYAKISSLLEKYKVQVPADLQRPFKVQGAEAGQKPFLTDEQQS
                     SLKNSSANFFREMANLNFTLWDLQQYKDQFKNKNPKPGDNPVEPKIAWKGEF"
     gene            684..2768
                     /gene="ppk1"
                     /locus_tag="PLU11_11960"
     CDS             684..2768
                     /gene="ppk1"
                     /locus_tag="PLU11_11960"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPN59887.1"
                     /translation="MNTERNIYLNRDLSWLSFNGRVLEEAADPTVALYNRFLFLSIFS
                     SNLDEFFRVRMPAIFAFSGLTTKKISLEEEYPAGLVQQVQQTIQLQQEEFGRILRDEL
                     IPALKSHQIYFCYDEPLPVHHLAAIREYFLSTVMSFLQPVFIHRIPPGSVFLENNALY
                     FILESESGERPGEPEYILLNIPSAALPRFLELPAGEGWSSILFLDDIIRACLTDVFPD
                     RTIQRAWSVKLTRNADMNLEDEFAGNLAEKIEKQLEKRDAGPATRLLVDGAMPERARL
                     FVLSFFELDQREIVMGGRYHHLKDLAGIPVAKRPGLAEEAWPPLSHPGFDLQVPVIES
                     IRKGDQLLHIPYHSYHPILRFFNEAALDPAVREIYITLYRVAPDSHIVHALMSAARNG
                     KSVTVFVELKARFDEANNLKWSKKMKAAGIKIISSIPTLKVHAKVAMLRRMEKGEMVD
                     YCYLGTGNFNELTGRFYTDHGFFTASKAVGQELQLLFSYLLSGKQPEVYGKIPFHHLL
                     VSQFNLVRKFDKLIRQEISRANEGRPAGIIIKMNNLQERSMIDRLYEASRAGVKIQLL
                     VRGICCLAPGVEGQSENITVTRIVDRYLEHARVFVFHNDGEPVYIMGSADWMNRNLHS
                     RIEVCFKVEEPRLKSEIATILDLQLKDNCKASYLIPQMENKRVLNDAPRFQAQEAIYQ
                     WLTQSVASAAGQ"
     gene            2810..3706
                     /locus_tag="PLU11_11965"
     CDS             2810..3706
                     /locus_tag="PLU11_11965"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPN59888.1"
                     /translation="MHFEWKGVFPALLTPFTAHDEVDLPMFGKNLQAQLDAGIQGVII
                     GGSLGEASTITVAEKEQLVKYALDYVKGKIPVILNIAESTTKDAIEQAANAENWGADG
                     LMLLPPMRYKSDDRETVTYFKTVARSTRLPIMIYNNPVDYKIDAHPALFDQLLECENI
                     TALKESSRDVTNLIRMKNRFGDRFKILCGVDTLAMEELCLEADGLVAGLVCAFPKETM
                     AIYNLVKAGKIAEAALIYRWFMPLLELDIHPKLVQYIKLAATQTGISNEYVRAPRLVL
                     EGEERERVLGIIKQGIATRPAL"
     gene            3837..>4294
                     /locus_tag="PLU11_11970"
     CDS             3837..>4294
                     /locus_tag="PLU11_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN59889.1"
                     /translation="MTNDATIQEINEVMSASWKAFHVYRKLSLKQRAGFMRAIAKELE
                     AAGDELIQTAMQETNLPEARLRGERGRTIYQLESYAEACEKGNWLEARIDHANPGKTP
                     PKPDIRKMLVPVGPVVVFGASNFPFAYSTAGGDTATAFAAGCPVIVKAHPA"
BASE COUNT         1139 a          934 c         1091 g         1130 t
ORIGIN      
        1 caaattcccc cttccaggca atcttcggtt ctaccggatt gtcaccgggt ttagggttct
       61 tatttttaaa ctggtcttta tattgctgaa ggtcccataa cgtaaaattc aggttagcca
      121 tttcgcggaa gaaattggcc gatgaatttt tcagagagga ctgctgttca tctgtcagga
      181 agggtttttg acccgcttcg gctccctgta ctttaaatgg acgttgcaga tctgcaggaa
      241 cctgaacttt gtatttttcc agcaagctgc tgatttttgc atatttctca tcggaaatgg
      301 tggttgtggt gcctctgaat ttgagatgaa actggtagcc tcctgcttct ttgcccattt
      361 tttccaggtc ttcgggtagt tgtgcggtac tttttaaaga gagtaaaaga agtccggcca
      421 ggatagtaaa acccccctgc cgcaggtatg gaagaggtag cataaggaga tagtttaggg
      481 tagtgtcctg taacaatcta cacccgccac cagccctgtt gtattgatag ctgtcacaac
      541 aactactgat ttcggtcacg aaactgctta ctgacttaaa agatatacag gtaccggatt
      601 tttcctggtt acctaattgt tatggaacag gttacggtaa cagggttttt taatttcagt
      661 atcttagttt aaagaacggc tgcatgaata ccgaacggaa tatttatctt aacagggacc
      721 tgagctggct gagttttaac ggaagggttt tagaggaagc ggccgatcct actgttgcct
      781 tgtataaccg cttcctgttt ctgtccatct tctcttccaa tctcgatgag tttttccggg
      841 tgcgtatgcc ggcgattttt gctttcagcg gactgactac caaaaaaatc tccctggaag
      901 aggaatatcc tgcaggactg gtgcaacagg tacagcaaac gatacagtta cagcaggaag
      961 aatttggccg gattctccgg gatgaactga ttccggcact caaaagtcat caaatttatt
     1021 tctgttacga tgaaccgctg cctgtccatc acctggcagc catccgcgaa tattttttat
     1081 ccacggtcat gtcatttctc cagccggtat ttatacaccg gataccgccc ggatcagttt
     1141 tcctggagaa caatgcgctt tattttatac tggaatcgga atccggagaa agaccgggtg
     1201 aaccggaata tatcctgctg aatattcctt cggcggcact gccccgtttc cttgaacttc
     1261 ctgcgggaga aggatggagc agtattcttt ttctggatga tattatcagg gcatgtctga
     1321 cagatgtatt ccccgacagg acgatacaga gagcctggtc ggtcaagctc acccgtaatg
     1381 cagatatgaa cctggaagat gagtttgccg gtaatcttgc tgaaaaaata gaaaagcaat
     1441 tggagaaacg ggatgccggt ccggccaccc ggttgctggt agatggagcg atgccggaaa
     1501 gagcccgtct gtttgttttg tcattttttg aattggatca acgggaaatc gttatgggtg
     1561 gaaggtatca tcatctgaaa gatctcgcag gcattccggt ggcaaaacgt ccgggcttag
     1621 cagaagaagc ctggcctcca ttgtcacatc cgggctttga tttacaggtg cccgtaatcg
     1681 aaagtatccg gaaaggtgac cagcttctgc atatacctta tcattcctat cacccgatcc
     1741 ttcgtttttt taatgaagcc gcccttgatc cagccgtcag ggaaatttat atcacccttt
     1801 accgggtagc tccggattca catattgtcc atgcgctgat gagtgcggcg cggaatggaa
     1861 agtcggtgac ggtttttgtg gaactgaaag cccgctttga tgaggccaat aacctgaaat
     1921 ggtcgaagaa gatgaaggca gccggtatta agatcatcag cagtatacct acactgaaag
     1981 tacatgcgaa agtagcgatg ctacgacgga tggaaaaggg agagatggtg gattattgct
     2041 acctgggcac cggtaatttc aatgaactga ccggccggtt ttataccgac catggatttt
     2101 tcacggcctc caaagccgtc ggacaggaac tgcagttgtt attcagttac ctgttatcgg
     2161 gtaaacaacc ggaagtttat ggcaaaattc cctttcatca tttactggtg tcacaattca
     2221 acctggttag gaaatttgac aaactgatcc ggcaggagat cagtcgtgct aatgaaggcc
     2281 ggccggctgg aattatcatt aaaatgaata acctgcagga acgatccatg attgaccggt
     2341 tatatgaggc cagccgggca ggggtgaaaa tacaattact ggtaaggggc atctgttgcc
     2401 tggccccggg ggtggaaggg caaagtgaaa atattacggt gacccgaatc gttgatcgtt
     2461 acctggaaca tgcgcgtgtc tttgtttttc acaatgacgg ggaaccggtc tatatcatgg
     2521 gtagtgccga ttggatgaac cggaacctgc atagccggat tgaagtttgt ttcaaggtgg
     2581 aagaaccccg gctgaaatcg gaaatagcca ccatactgga cttacaattg aaggataatt
     2641 gtaaagccag ttatctcatt ccacaaatgg agaataagcg tgttttgaat gacgctcccc
     2701 gttttcaggc acaggaggcg atctatcaat ggttgacgca atcggttgct tctgctgcgg
     2761 gacagtaaaa attgctgaat cccttatctt tagtcctaaa acaactgata tgcattttga
     2821 atggaaaggc gtttttcccg cactactgac cccgtttaca gcccatgatg aagtggatct
     2881 tcccatgttt ggaaaaaacc tgcaggccca gctcgatgcc gggatacaag gggttattat
     2941 tggggggtca ttgggtgagg ccagtaccat taccgtggcc gaaaaagaac aactcgtaaa
     3001 atatgcactg gattatgtaa agggaaagat cccggtgatc ctgaatattg cagaaagtac
     3061 cacaaaggat gcgatcgaac aggcggccaa tgcagaaaac tggggtgccg acggactgat
     3121 gctgttgccc ccgatgcggt ataaatccga tgaccgggaa accgtcactt atttcaaaac
     3181 ggttgcccgt tccacccggc ttccgattat gatttataat aacccggttg attataagat
     3241 tgatgcacat cccgctttgt ttgatcagtt gctggaatgt gaaaatatta ccgcgcttaa
     3301 agaaagttca cgtgatgtga ccaacctgat ccggatgaag aacaggtttg gtgaccgctt
     3361 taaaattctc tgtggagtgg atacgctggc aatggaagag ttatgcctgg aagcagatgg
     3421 gttggttgcc ggactggtat gtgccttccc gaaagaaacc atggccatct ataacctggt
     3481 aaaagcaggt aagattgctg aagctgcctt gatataccgc tggtttatgc ctttgctgga
     3541 actcgatatt catcccaagc tggtacaata cattaaactg gccgcaaccc agaccggcat
     3601 cagtaatgaa tatgtacggg cgccaagact ggtgttggaa ggagaggaaa gagaacgggt
     3661 gctggggatt ataaagcagg ggattgcaac cagaccggct ttataagtaa aaagtaaaaa
     3721 gtaaaaagta aaaaacccaa tagaggtaga atcggtatag ttaacaaaac tctctaagga
     3781 ggggctgttc ccttctcgct tctcgcttct agcctctcgc ttctaactaa cgactaatga
     3841 caaacgacgc aaccatacaa gagatcaacg aagtgatgtc cgcttcctgg aaagcttttc
     3901 atgtttaccg gaaattatca ctgaaacagc gggccggatt catgcgagcg attgcaaaag
     3961 aactggaagc tgccggtgat gagctgattc agaccgccat gcaggaaacc aatctgccgg
     4021 aagcccggct gagaggagag cgtggtcgca caatctatca gctcgaaagt tatgcggaag
     4081 cctgtgagaa aggtaattgg ctggaagcac gcattgatca tgccaatcct gggaagacgc
     4141 cgcctaaacc ggatatccgc aagatgcttg ttccggttgg tccggtagtg gttttcggag
     4201 ccagtaattt tccttttgct tactctactg ctggcggaga cacggctact gcttttgccg
     4261 cgggttgtcc cgtgattgtg aaagcacatc cggc
//
LOCUS       DAOPMW010000284         4267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24356_length_4267_cov_6.629867, whole genome shotgun sequence.
ACCESSION   DAOPMW010000284 DAOPMW010000000
VERSION     DAOPMW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24356_length_4267_cov_6.629867"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /locus_tag="PLU11_11975"
     CDS             <1..2171
                     /locus_tag="PLU11_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN59890.1"
                     /translation="ATALPRFPDAPVTMALFIILSFNTKLVKIRRQLKPAPVRYSLVN
                     YTTARYMLKQILILFYLLLLALQPMAQQNCTEPGQTPVSAIPVCGSEPFTITTPTYCG
                     ITNIPVPCAGGFSYQNTNPNFFRMACFSSGTLGFLITPSVATANYDWQLFDITSRNPF
                     DIFTNPDAFVACNWSGEPGETGASDDGTELIVCAGSGMNTYSKMPQVVQGRTYLLMVA
                     NQSASGGSYQLTLTGGTASITDAIDPKMDYAMMSCDGQKITLRLNKKIVCGSIAADGS
                     DFSLSNGTAIISAAAADCSSPFGSDSIFIRLAQPLPNGNYTLRINNGTDNNTLVDVCN
                     KNIAAGQTLAFSVSDQQRTYADEAVIFSCAPGYVRVGFNRPIRCGSLLADGSQFNITG
                     PQSVTVIAAEALNCGPSLLSNEVILRFATAVNLAGIYEIRLNPAGSNGSTLLDECGIA
                     PVNSSAIPYFTSSNPVSARFSFIKNETCKGSQFLFSHNGANAVNNWSWIFGNTGTSNL
                     QHPVYSFTSGGQHQVQLVVSNGACSDTIRQTLNSIQTVKAAFETDALVCPGDTLTFKN
                     NSSGSIDRWEWNFGNGSTNTLPLPRNFQFDPVPAETLYPITLVAYNNALNCSDTARQL
                     VRVLNSCIIAVPTAFTPNGDGLNDFLYPLNTERAEQTRFSVYNRNGQLVFHSTDRSGK
                     WDGKVNGKGQDAGVYVWIFSYTTAQSKQPVLLKGTTLLIR"
     gene            2227..4143
                     /locus_tag="PLU11_11980"
     CDS             2227..4143
                     /locus_tag="PLU11_11980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPN59891.1"
                     /translation="MQSTLKVLLLTLISVSSFAQQNFSFTPEKPKPGETISITYRPAG
                     DIANTILPVEAIVYQHGQSSQKADDLLLKKTADGFTASFIADTAANFIYFSFSADKKF
                     DNNANEGYIIHLYENDKVRKGSNINQFMFYQFYGSGAGVERNNEKALAAMETEFSLYP
                     DNRKPWLGNYIRLKNTVKKDATNALALQEIERLIKGGLKEETDYETLAGLYSLAKLPE
                     QSKWISQLWKEKYPDGKWTVNETLQAYYTQPDPEKREVMLNEIIQKTKTDKKWETVIQ
                     SIPRLQTIIVSGYATKKNWEKMRAYAAKIEDKATLAQTYNSAAWEMQKKGENLEIAEE
                     MSWFATEYSREQWLKPTAKKPESMTEKQWKKNAEFSYAQNADTYGMVLYRMGQFKKGL
                     PFAKEAAIKIQQGKDADYNNTYALLAEKVMPAKKLKPELEQFVKEAKSTSEMTEILKR
                     IYTSEKKTEDGFEQYITALKEESRLKMLKDIQQSMLNENSPSFALVNLEGKKVDIAEL
                     KGKVVVVDFWATWCGPCIASFPGMQKMVTKYKDNPDVKFVFVDTWETKEEKVKNAQEF
                     ISSNKYTFDVLMDNDNKVVEQFKVDGIPTKFVIDKNGVIRFKSVGFSGSDDKLIAELT
                     AMIDIAANPPSKSF"
BASE COUNT         1275 a          964 c         1006 g         1022 t
ORIGIN      
        1 ctgccactgc ccttcccagg tttccggatg cgccggttac gatggccctt ttcattattc
       61 ttagttttaa tactaaatta gtaaaaatcc gcaggcagct aaagccggca cctgttcgat
      121 atagtttagt aaattacaca accgcccggt acatgctgaa gcaaatcctg atattgtttt
      181 accttttgct gctggctttg cagccaatgg cccagcaaaa ctgtacagaa ccgggacaga
      241 ctccggtttc cgccataccg gtctgtggca gtgagccatt taccattact actccaacct
      301 attgcggcat caccaatatt ccggtcccct gtgccggagg tttcagttat cagaatacca
      361 atcccaactt tttccggatg gcctgcttca gttccggcac cctgggtttc ctgataacac
      421 cctctgtagc caccgccaat tacgactggc agctttttga tatcacgagc cggaatccgt
      481 ttgacatttt caccaacccg gatgcatttg tagcctgtaa ctggtccggc gaacccggtg
      541 aaacaggcgc atccgatgat ggaacagaac taattgtttg tgccggctct ggtatgaata
      601 cctatagtaa aatgccccag gtcgttcagg gtcgtactta tctgctgatg gtagccaatc
      661 aaagtgcttc aggtggcagt tatcaactga cccttaccgg gggaacggcc agtataacgg
      721 atgctattga tcctaaaatg gattatgcca tgatgagttg tgatggacag aagatcaccc
      781 tgagactgaa taagaagatc gtatgtggca gtatagcagc tgacggttcg gactttagtc
      841 tgagtaacgg cacagcaatc atttcagcgg cagctgcaga ttgcagttca ccatttggca
      901 gtgattccat atttatccgg ctggcacaac cccttccaaa tggcaattat accttacgta
      961 tcaataacgg gacagataat aatacgctgg tagatgtatg taataaaaat attgcggccg
     1021 gtcagacgct tgccttttca gtttccgatc agcagcggac ctatgcggat gaagcagtca
     1081 tcttttcctg cgcacccggg tatgtccggg taggattcaa ccggcccatc cgttgtggca
     1141 gcctgctggc tgatgggagt caattcaaca taaccggtcc tcaatctgta acagtgattg
     1201 cggcagaagc gctcaattgt ggaccttcac ttctcagcaa tgaagtgata ctccgtttcg
     1261 ccacagctgt gaacctggcc gggatctatg aaatacgatt aaatcctgcg ggaagcaatg
     1321 ggagtacttt actggatgaa tgtggtattg ctccggtcaa ttcatctgcc attccttact
     1381 tcaccagcag caacccggta tccgcccggt ttagttttat aaaaaatgaa acctgcaaag
     1441 gaagccagtt tctcttctcc cataatgggg ccaatgcggt caataactgg tcctggattt
     1501 ttggtaacac cggcaccagc aatcttcagc atccggtcta ttcttttact tcaggtggcc
     1561 agcaccaggt gcagcttgtg gtatcgaacg gagcctgttc ggatacgatc cggcagacat
     1621 tgaacagtat tcaaacggtt aaagctgctt ttgaaacgga tgccctggtg tgcccgggtg
     1681 atacattaac ttttaagaat aacagttccg gcagtattga ccggtgggaa tggaatttcg
     1741 ggaatggaag caccaataca ctgcccctgc cccgcaactt tcagtttgac ccggtaccgg
     1801 cagaaacact ttatcccatt accctggtgg cgtataacaa tgccctgaat tgcagtgata
     1861 ccgcccgcca gctggtgagg gtactgaaca gttgtatcat cgcggtgccc accgctttca
     1921 cgccaaacgg agatggatta aatgactttc tctacccgtt gaatacggag cgtgctgagc
     1981 aaactcgttt tagtgtatac aatcgcaatg gtcagctcgt ctttcatagc acggaccgtt
     2041 ccggtaaatg ggacggaaaa gtaaatggca aggggcagga tgccggtgta tatgtatgga
     2101 tattcagtta taccactgct caaagtaagc agcctgtctt gctaaaagga accaccctgc
     2161 tgatccggta attttaacat taaacacgaa gggatttgtg ttaaattgct ccaaaaacca
     2221 aaagccatgc aatctactct caaggtattg ctcctgaccc tcatttccgt cagcagcttt
     2281 gcccagcaaa atttcagctt tacgccagaa aagccaaaac ccggagaaac catcagcatc
     2341 acgtaccgtc cagccgggga tattgccaat acgatactac cggtggaagc catcgtttac
     2401 cagcatggac agagttcaca aaaagcggat gacctgctcc tcaaaaaaac agcggatgga
     2461 tttaccgcca gttttatagc ggatacggca gctaacttta tttacttcag ttttagtgcc
     2521 gacaaaaagt ttgataataa tgccaatgaa ggatacatca ttcatttgta tgaaaatgat
     2581 aaagtacgca aaggcagtaa tatcaatcag tttatgtttt accagtttta tggcagcggg
     2641 gccggagtgg aacgaaataa tgaaaaagcg ctggccgcca tggaaactga gttcagctta
     2701 tacccggata accggaaacc ctggttggga aactatatac ggcttaagaa cacggtaaaa
     2761 aaggatgcaa caaatgccct ggccttacaa gagatcgagc gtctgatcaa aggcggactg
     2821 aaagaagaaa ctgactatga aaccctggct ggcctgtaca gtcttgcaaa actgccggaa
     2881 caatcgaaat ggatttccca actctggaaa gaaaaatatc cggacggcaa gtggactgtc
     2941 aatgaaacac tacaggcgta ttatacacaa cctgacccgg aaaaaaggga agtgatgtta
     3001 aatgaaatca tacagaaaac gaagacggat aaaaagtggg aaacagtcat tcaaagtatt
     3061 ccccgtttgc agacaatcat tgtaagcggg tatgccacaa agaagaactg ggagaaaatg
     3121 agagcgtatg cagcaaaaat tgaagacaag gcaacgctgg ctcaaaccta caattccgcc
     3181 gcctgggaaa tgcagaagaa aggcgaaaac cttgagatag cagaggaaat gtcctggttt
     3241 gctacggaat acagtcgcga acagtggctc aaaccaacgg caaaaaaacc ggaaagcatg
     3301 accgagaagc aatggaagaa aaatgccgaa ttcagttatg cgcagaacgc ggatacctat
     3361 ggcatggtgc tgtaccggat gggacagttc aaaaaaggac ttccctttgc caaagaagct
     3421 gcgattaaaa ttcaacaggg aaaggatgcg gattataata atacctatgc cctgctggca
     3481 gaaaaagtaa tgccggccaa aaaactgaaa ccggaactgg agcaatttgt aaaagaggca
     3541 aaatccacca gtgaaatgac ggaaatcctg aaaaggatct ataccagtga aaagaaaaca
     3601 gaagatggtt tcgagcaata tatcacagcg ttgaaagaag aaagccggtt gaaaatgctg
     3661 aaagatattc aacaatccat gttgaatgaa aacagtcctt cttttgccct ggtcaacctg
     3721 gaaggcaaaa aagtggatat cgcagaactg aaaggcaaag tagtggtggt ggatttctgg
     3781 gccacctggt gcggcccctg tattgccagt ttcccgggta tgcagaaaat ggtgactaaa
     3841 tacaaagaca acccagatgt gaaatttgta tttgttgaca cctgggaaac caaagaagag
     3901 aaagtgaaaa atgcgcagga atttatcagc agcaataaat acacttttga tgtgctgatg
     3961 gacaatgaca acaaagtggt ggagcaattc aaagtggacg gcattcccac caagtttgta
     4021 attgataaga acggggtgat ccgtttcaaa tccgttggat tcagtggcag tgatgataag
     4081 ctgatcgccg aactgactgc gatgatcgat attgctgcga atccgcccag caaatcattc
     4141 tgataaagag aataggcggc ttcgccgcag ttttaacaca tagattcata gggacatagg
     4201 agacacatag caagaaattg tatttctatg tgtctcctat ttattctaac agagctccct
     4261 gctttag
//
LOCUS       DAOPMW010000285         4247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24529_length_4247_cov_9.367366, whole genome shotgun sequence.
ACCESSION   DAOPMW010000285 DAOPMW010000000
VERSION     DAOPMW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24529_length_4247_cov_9.367366"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1249)
                     /locus_tag="PLU11_11985"
     CDS             complement(338..1249)
                     /locus_tag="PLU11_11985"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HPN59892.1"
                     /translation="MYRVLLFVFVLSTFTVHAQRELFLDITAHNDFFNYQGTDKYYTG
                     GHRIGLSFRNPKKADRFHSVAVQQKIYTPSEISVPGYTAGDYPYNGLLYLSYRQGRLF
                     RQGKASVTVQVDAGTTGGSSAAGEVQTGFHKLIQDQLPRGWHQVLELGELLQVQLDFR
                     RLVYQQKGVALTMLKSFQTGTIYNRLQFGVEVKAGNDVFPFLRQRGAFATGSSKKTGF
                     YFFASPVLSYVHRNRMFDTRQLQIETAAAPASTDLETEELVGTLNTGFVYFSPGFQLT
                     LSQYMNTPEFRGATTHTYGEIGLQFRL"
     gene            complement(1455..2363)
                     /locus_tag="PLU11_11990"
     CDS             complement(1455..2363)
                     /locus_tag="PLU11_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN59893.1"
                     /translation="MPETTKRKSVFNHAALNMPEGAADAYTKGFSVHTFMTEEQLAHY
                     RRQSRSDFIAIVLVTEGELVCKVNLRECVARKNNVVLLSPGQVKQLVHLEPGTRISML
                     SFTPGFITAMGFPKQFSELVEYLSSATDQLWELESGDMRTLMVLLRQLSAYTKQTSAH
                     NYGKELLYHQFSIFLYEMAGFRKKYTQRLTAVVSRKENLLLRFSSLVKKHYKKQRSVL
                     AYARQLHVTAKYLSATVKELTGQTAGEMIDEQVLLEARYCLHDPDTTIAEIAHHLNFP
                     DPSVFGKFFKRHTGVTPMSYRRQQLM"
     gene            2563..3063
                     /locus_tag="PLU11_11995"
     CDS             2563..3063
                     /locus_tag="PLU11_11995"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPN59894.1"
                     /translation="MEENLSGPQEEQHLFDNFQSVALTQAGSGKRLANYIVDLISFYV
                     FMYFFSYVIVEVSMDLAIIMYAEPEYGGRPLLAQLINMGFYGFYMGLLETLFRGRSLG
                     KLITGTIAVNEDGTRINGRKALLRGLCRAVPFNALSALGTPCYPWHDKWNNTYVVGYA
                     DFSANQ"
     gene            complement(3142..>4247)
                     /locus_tag="PLU11_12000"
     CDS             complement(3142..>4247)
                     /locus_tag="PLU11_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPN59895.1"
                     /translation="ICTEFYPNNPDGLIGNEDCGQMSAWLVLSAMGFYPVTPGSDVYA
                     LGTPLFDKIDISLENGKHIYIKAKNHGPENFYVESMQLNGKDHPASFFRHSDLANGAE
                     ILFNMSDKPALKRGIKDADMPHSKVADENFVAVPYFDMESYKFKSTAQVKLAHIDPAA
                     KIYYEVKSEHKQVHTFPTYIKPFSLTKTDTVVAWAMKNGISSPRIYRVFYKVPANISI
                     EVKSKVHPMYTAGGKEALIDGIMGNENWKTGDWQSYYDQDFKAVIDLHTVRPVKYVGV
                     HVLQEVSPWIVYPQELMVETSNDGKTYQFLTVIKNEVGTDVKGPVHQVLGQYVNAKTR
                     YLRFTALTGGPLPAWHESAGSGSHVFVDEVIVK"
BASE COUNT         1084 a         1064 c          965 g         1134 t
ORIGIN      
        1 aacaatggat gaacggattg aatctgccag agagcaaatt attgaaaact atattataaa
       61 atggtcaggg gattagagaa gttcagggag tattttactg actttccgga tagctatata
      121 atcatcggag gtactgcttt agatagggtg atatgcaaat tggaagaaaa gcagatatgg
      181 ccgctttatt tgaacagttt aaaacaaact ttctgtctga tttttcataa aagccattga
      241 tactttccgc tttaggtgca aagaatcttt cctcaccctt cggcacgcct gcctgtccgg
      301 taggcaggga gagggggcag ggggtgaggt aatgcaatca caaccgaaac tgcaacccga
      361 tctccccata cgtatgagta gtggcacccc tgaactccgg tgtattcata tactgggaaa
      421 gcgtgagctg aaagcctggt gagaaataga caaagcctgt gttgagcgta ccaaccaact
      481 cttctgtctc cagatccgtg ctggcaggag ctgcggccgt ttcaatctgc aactgccgcg
      541 tatcaaacat ccggttgcgg tgcacataac tcagcaccgg actggcgaaa aaataaaacc
      601 ctgttttctt actgctcccc gtggcaaatg caccccgctg ccgcagaaaa ggaaagacat
      661 cattccccgc ttttacttct accccaaatt gcaagcgatt ataaatggtt ccggtctgga
      721 atgatttcag catggtaagc gccactccct tctgctgata caccagccgg cggaaatcga
      781 gttgcacctg taacaactca ccgagctcca gcacctggtg ccagccacgt ggcaactgat
      841 cctgtatgag tttatgaaaa ccggtctgca cttcccctgc cgcagatgat ccgcccgtgg
      901 tacccgcatc cacctgcacc gtaacactgg ccttgccctg gcggaataaa cggccctgcc
      961 ggtaactgag gtataataaa ccattgtacg gataatcacc cgccgtataa cccggtaccg
     1021 aaatctccga tggcgtatag atcttctgct gtaccgccac cgaatgaaaa cgatccgctt
     1081 tcttcggatt gcggaaactc agaccaatgc ggtgtccgcc ggtatagtac ttgtctgtac
     1141 cctggtaatt gaaaaaatca ttgtgcgcgg taatatccag gaatagctcg cgctgggcat
     1201 ggaccgtaaa cgtgctcagg acaaaaacaa aaagtaaaac tctgtacata ggcgtttatt
     1261 tgaggcacag gattccctgt tccgctcttc ccttcgctcc ggaaccctgt tagcccgttg
     1321 ttctataatt cgggtaaaag gacaatgaac acattgtccg ctgtatgctg ttcaggttac
     1381 aaaattgcac ctgaaagagc ctttcaaaaa gggagaaagg gcggcagttc tggtcgaaat
     1441 gtcgcttttg ttgtttacat caactgctga cggcgataag acatgggcgt gacacccgta
     1501 tgccgtttga agaatttacc aaagacagaa ggatctggaa aattgaggtg atgggcaatc
     1561 tctgcaatcg tggtatccgg atcatgcagg caataccggg cttcgagcaa tacctgttca
     1621 tcaatcatct caccggctgt ctgcccggtt agttctttta ctgttgctga aagatacttg
     1681 gcggttacat gcagctgacg ggcataggcc agcacggacc gctgtttttt atagtgcttc
     1741 tttaccaagg atgaaaaccg caacaggagg ttctccttcc gggacaccac agccgttaat
     1801 cgttgggtgt acttcttccg gaaaccggcc atctcatata aaaaaatgga aaactggtga
     1861 tacagaagtt ccttcccgta attatgcgca ctggtctgtt ttgtataggc tgaaagctgc
     1921 cgcagcaaga ccatcagggt acgcatatcg ccggactcca gttcccataa ctggtcagtc
     1981 gccgaagaaa gatactccac cagctcactg aactgtttgg gaaatcccat cgccgtaata
     2041 aaaccggggg taaaactcag catggaaatc cgggtacccg gctccaggtg cacgagttgc
     2101 ttgacctgtc ccggtgataa taaaaccaca ttgttcttac gggccacaca ttcccggaga
     2161 ttcactttgc agaccagttc cccttcggta accagtacaa tggcgataaa atcagaacgt
     2221 gactgacgac ggtaatgagc cagctgctcc tccgtcataa atgtatgtac cgaaaacccc
     2281 ttggtatagg catccgccgc cccttctggc atatttaagg ccgcgtgatt aaaaacggat
     2341 tttcttttcg tggtttcggg cattcgtgcc ggacagtagg ttagtccggg gcaaattaac
     2401 aacaggcggc tttcagatat ggggacttat atcacaatcg ctacgtgcag cgtcaccttt
     2461 ttgtataatt atgtttgtaa taaatccaga tcaatttcaa cagggcctta tccttttatt
     2521 ttgagaattt attatcttgg gccgaaccaa aactttatcc gcatggaaga aaatttatcc
     2581 ggcccgcagg aagaacaaca cctctttgat aatttccagt ccgtcgccct gacacaagcc
     2641 ggttccggta aacgactggc caattatatt gtcgatctga tttcattcta tgttttcatg
     2701 tactttttca gctatgtgat cgtagaggtt agtatggacc tcgccattat aatgtatgct
     2761 gaacctgaat atggaggacg tcccctcctt gctcaactga tcaatatggg cttttacggc
     2821 ttctatatgg gactgctgga aactttgttc cgtggccgct ccctgggcaa actgattact
     2881 ggtaccatcg cggtgaatga agatggcaca cgcatcaacg gcagaaaagc cctgctccgc
     2941 ggactgtgcc gtgcggttcc ctttaatgca ctgagtgctt tgggcactcc ctgctatccc
     3001 tggcatgaca agtggaacaa tacctatgta gtgggatacg ctgatttttc ggctaaccag
     3061 taataagatc gtttggctct tccgtattag aactgcatct gtactgaaga gccttcccgc
     3121 tctctactca ttactgatta ctcatttaac tatcacctca tccacaaaca catgactccc
     3181 gctccccgca ctctcatgcc aggccggtaa cgggccgcct gttagcgcgg tgaaacggag
     3241 atatcgggtt tttgcattga cgtactgacc gagtacctgg tgtaccggtc ctttgacatc
     3301 cgtacccact tcatttttta taacggttaa aaactggtag gtcttgccat cgttgctggt
     3361 ctctaccatc agttcttggg gatatacaat ccagggactc acttcctgca acacatgcac
     3421 gcctacatat ttcacgggac gaacagtatg caggtcgatc acagctttga aatcctggtc
     3481 ataataactc tgccagtcac cggtcttcca gttctcattg cccatgattc cgtcaatcaa
     3541 cgcttcctta ccacccgcgg tatacatggg atgtaccttg ctctttacct caatactgat
     3601 attggcgggt actttataga agacccggta gatacgggga ctgctgatgc cattcttcat
     3661 ggcccaggca actaccgtat cggttttagt taaactgaat ggttttatgt aagtgggaaa
     3721 ggtatgtacc tgtttgtgct cactctttac ttcataataa attttcgccg ccggatcaat
     3781 atgcgccaat ttcacctgtg ctgtggactt aaacttataa ctctccatat caaaataagg
     3841 gaccgctaca aagttttcat cggctacctt actatgtggc atatctgcat cttttattcc
     3901 acgcttcagg gccggcttgt ccgacatatt gaaaaggatc tcggccccat tggccaggtc
     3961 actgtggcgg aaaaagctgg cgggatggtc tttgccattg agctgcatac tttccacata
     4021 aaaattttcc gggccgtggt ttttggcctt gatataaata tgtttcccgt tttccaggct
     4081 gatatcgatc ttatcaaata agggtgtacc gagtgcataa acatcactgc cgggggtcac
     4141 aggataaaaa cccattgcac ttaatacgag ccaggcactc atttgtccgc agtcctcatt
     4201 gccaatcagt ccatccgggt tattgggata aaactccgtg cagatct
//
LOCUS       DAOPMW010000286         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24569_length_4242_cov_8.969190, whole genome shotgun sequence.
ACCESSION   DAOPMW010000286 DAOPMW010000000
VERSION     DAOPMW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24569_length_4242_cov_8.969190"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1115
                     /locus_tag="PLU11_12005"
     CDS             231..1115
                     /locus_tag="PLU11_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HPN59896.1"
                     /translation="MISYNPRDWFTFIIRFHKADTFRQLLPLIIGISIYTGAVAWLEI
                     YYWQLPEKHYIRNIPVMHTLLGFAISMLLVFRTNTAYDRWWEGRKLWGALVNNSRNLA
                     MKLETILPEGDLKSRTFFRKAIPAFASALHHHLHKEQTRLELFEDEENRHLFQKIDWE
                     KHVPNQVALLMYRQVQQLYREGVLTAEQLLFLNAELQSFTDICGACERIKNTPIPFSY
                     SVFIKKFIFFYVMTLPFGFVFQLGFYAIPVTAFVFYVLASLELIAEEIEDPFGGDAND
                     VPTETLSRNIHRHVGELI"
     gene            1250..1618
                     /locus_tag="PLU11_12010"
     CDS             1250..1618
                     /locus_tag="PLU11_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59897.1"
                     /translation="MKQQRFILVLPLITFFLYACSKESNGSNNSGGGALDCSAVVNKN
                     FAADVNPIIQSSCATTSSCHGSGSVNGPGALTSFTAIFNSRNSIRSAVSSGSMPKNSS
                     LSTAQKNSILCWIDSGASDN"
     gene            1695..4115
                     /locus_tag="PLU11_12015"
     CDS             1695..4115
                     /locus_tag="PLU11_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN59898.1"
                     /translation="MKKLAVLFLLFLYNCAGAQTGLSPLSVEKIMRDPRWMGSSPSSP
                     SWSNDGKRLYFMWNPDKAPADSLYYITLTDKKPVKASVKEKVEYNATGNFIYNQARTA
                     YLFSKNGDIFYTDTKTGRVRQITQTLEAENNPQFGFNETRIIYTRNQNLYSWDIMGGE
                     TIQLTNIRTGESTTAPAAGGFQRSGAPPASARAGANPQEQWLQQDQLHWFQVLKERKK
                     KREMAEAYTKSVPKEKELRSINLEDKMLQGLSISPDGRYISYRLQKAAGSKSTIVPSF
                     VTETGFTVDLPARSKVGTLQGLSEMYLYDREADTIYQVRTDSIPGIKDLPDYVKDYPK
                     LLEEKTRKSALRPVNFGSLNWSPKGSNAVLEIRSQDNKDRWLMKFDRDKGIFSLLDRQ
                     RDEAWIGGPGTGGFGSASSGWIDDQLFWFRSEASGYSHIYLVNISTGEKRALTSGSYE
                     VQQVQISRDKKYFYITTNEVHPGEQHFYRLTIADGKKEKITTATGAHQVLVSPDEKWL
                     ADLYSYSTKPWELFLKENKTNGKEEQITFLGKSEEYNSYNWKDPEVVSFTARDGATVY
                     ARLYKPANPHPNKPAVLFVHGAGYLQNAHKWWSSYFREYMFNNLLADQGYVVLDIDYR
                     GSAGYGRDWRTGIYRHMGGKDLSDHVDAVNYLVKNHGINPQHVGLYGGSYGGFITLMG
                     MFNEPGVFAAGAALRPVTDWANYNHGYTSNILNEPFTDSLAYRKSSPYYFAEGLKGNL
                     LICHGMVDVNVHYQDAVKLAQRLIELGKDNWELASYPMEDHGFVEPSSWTDEYKRIFR
                     LFESVLKK"
BASE COUNT         1197 a          939 c          980 g         1126 t
ORIGIN      
        1 ggtggaatca ttaaggcgtt tgaagccatt aaagtggcgt caaccattaa aggggaaagg
       61 aaacctgaaa aaccggtcag taaagtaaaa gccaaaagca agcaataatc cattgatctg
      121 ataagcagaa agctgttcca tccgggacag cttttttttt gaagggttaa ctgtacatac
      181 atgtaaacaa ccatttaccc gattgcctta tttttgaaat aaaaaccgga atgatttctt
      241 ataatccccg tgactggttc acttttatta tccgatttca caaagcagat acgttcaggc
      301 agttactgcc attaattatt gggatcagta tctataccgg agcggtggcc tggctggaaa
      361 tttattactg gcagctcccg gagaaacact atatccgcaa tatcccggtg atgcataccc
      421 tcctggggtt cgcgatctcg atgttgctgg tattccgcac caataccgcc tatgaccgct
      481 ggtgggaagg ccgtaaatta tggggcgccc tggtcaacaa cagccgcaac ctggccatga
      541 aactggaaac cattcttccg gaaggagatc tgaaaagcag gacctttttc cggaaagcca
      601 ttccggcctt tgcctctgcc ctgcaccatc acctgcataa ggagcagacc cgtcttgaat
      661 tatttgaaga cgaggagaac cgtcatttat ttcaaaaaat tgactgggaa aaacatgtac
      721 ccaaccaggt cgcattattg atgtaccggc aggttcagca attataccgg gaaggggttt
      781 taacggcaga acaactactt tttctcaatg cggaactgca atcctttacg gatatatgcg
      841 gtgcctgtga acggatcaag aataccccta tccctttttc ctatagtgtg tttatcaaga
      901 aattcatatt cttttatgtc atgaccctgc cctttggatt tgtgtttcag ttgggattct
      961 atgctatccc tgtcactgcg tttgtttttt atgtactcgc gagcctggaa ctgattgctg
     1021 aagaaataga ggatccgttt ggcggagatg ccaatgatgt acctacagaa accctctcca
     1081 ggaatataca ccggcatgtg ggggaactga tctgacggaa cggattcgtt gatgtatacg
     1141 tgaatgccgg acttttcaat cgtaatcacc cttgtggttt ttgcttttta tcctggcagc
     1201 aggcaactta tttttcccga atccgttttg ataaaaaacg gatcaccgta tgaaacagca
     1261 acgattcatt cttgtcctcc cgctcattac tttttttcta tatgcctgca gtaaagaaag
     1321 taacgggagc aataactccg ggggtggagc cctggattgt tctgccgttg taaataaaaa
     1381 ttttgccgct gatgtaaatc caatcataca atccagctgc gccactactt cttcctgcca
     1441 tggaagtggc agtgtcaacg gccctggggc tttgaccagt tttaccgcca tttttaattc
     1501 gaggaacagt atccgttctg ccgtgtcttc gggttctatg cctaaaaaca gttcactcag
     1561 caccgctcag aaaaactcga tcctttgttg gattgacagt ggcgcatccg ataattaatc
     1621 ccgtttatcc agatttcaga ttcgtatacc cggtaatgct cattctataa tatatttata
     1681 gaaaaataag ggttatgaaa aaactggctg ttctctttct tttattcctg tacaattgtg
     1741 ccggggcaca aacgggttta tcaccgttgt ccgtagaaaa aatcatgcgg gatccccggt
     1801 ggatgggaag ttctccctcc tctccttcct ggagtaatga cggtaaacgg ctttatttta
     1861 tgtggaatcc tgataaagca ccggcagatt cattgtatta tatcacactg accgataaaa
     1921 agccggtaaa agcttccgta aaggaaaaag tggaatacaa cgccactggc aattttattt
     1981 ataaccaggc acggacagcc tacctgtttt ccaaaaacgg ggatattttt tatacggata
     2041 ctaaaacagg gcgtgtccgc cagatcactc aaacccttga agcggaaaat aatccccagt
     2101 ttggtttcaa tgagacccgc atcatatata cccggaatca gaatttgtat tcatgggata
     2161 tcatgggtgg agaaacgatt cagctaacca atatacgcac aggagaatcc accacagcgc
     2221 cggcagccgg tggctttcag cgaagcggtg ctccacctgc aagtgccaga gccggggcta
     2281 accctcagga acaatggctg caacaggacc aactccattg gttccaggta ttgaaagaaa
     2341 gaaagaaaaa aagggaaatg gctgaagctt ataccaagtc tgtacccaaa gaaaaagaat
     2401 tacgcagtat aaacctggaa gataaaatgc tgcaaggatt atccatcagt ccggatggcc
     2461 gttatatcag ttaccggttg caaaaggcag ccggttctaa atccactatt gtcccgagtt
     2521 ttgttacgga aaccgggttt accgtggatt tacccgcccg ttccaaagta ggcaccctgc
     2581 agggactgag tgaaatgtat ctctatgacc gcgaagccga cacgatctac caggtacgta
     2641 cagattctat ccccggtata aaagacctgc cggattatgt gaaggattat ccgaaactgc
     2701 tggaagaaaa aaccagaaaa tccgctttac gcccggtaaa cttcggatct ctgaactggt
     2761 cacctaaagg ctcgaatgcc gttcttgaaa tccgttcaca ggataataaa gaccggtggc
     2821 tgatgaaatt tgaccgggat aaaggcattt tcagtttact ggatcgtcag cgggatgaag
     2881 cctggatcgg gggacccggt accggcggct ttggcagtgc ctcctccgga tggattgatg
     2941 atcagctctt ctggttccgc tcagaagcca gcggctactc acatatctac ctggtaaata
     3001 tcagtacggg tgaaaaaaga gcgctcacat ccggcagtta tgaagtgcag caggtgcaga
     3061 tttcccggga taaaaaatat ttttatatca ccaccaatga agttcatccg ggtgagcaac
     3121 atttttaccg gctgactatt gccgatggga aaaaagagaa aatcaccact gctacggggg
     3181 cgcatcaggt gctggtttcc ccggatgaaa aatggctggc tgatttatac tcctattcca
     3241 caaaaccctg ggaattgttc ctgaaggaaa ataaaaccaa tggcaaagaa gagcagatta
     3301 cttttttagg aaagagcgag gaatataatt cctataactg gaaggatcct gaagtagtat
     3361 cttttactgc ccgggatgga gccacggtgt atgcccgttt atataaaccc gccaatcctc
     3421 atcccaataa accggctgta ctctttgtgc atggggccgg ttatctgcaa aatgcacata
     3481 aatggtggag cagttatttc agggagtata tgtttaataa cctgcttgcc gaccagggat
     3541 atgtagttct ggatattgat taccggggaa gtgccggata cggccgtgac tggagaaccg
     3601 gtatttatcg tcacatgggt ggaaaagatt tatccgatca tgtggatgcc gtgaactacc
     3661 tcgtcaaaaa tcacgggatc aatccgcaac atgtaggact ttacggaggc tcatacggag
     3721 gttttattac cttgatgggt atgttcaacg aacccggcgt ttttgctgcc ggagcggctt
     3781 tacggccggt taccgactgg gctaattata atcatggtta cacttccaat atcctgaatg
     3841 aaccttttac cgatagcctg gcttaccgga agagttcccc ttattacttt gccgaaggac
     3901 tcaaaggaaa cctgctgatc tgtcatggaa tggtggatgt aaatgtacat tatcaggatg
     3961 cggtaaaact cgcacaacgg ctgatcgagc tgggaaaaga taactgggaa ctggcttcct
     4021 atccgatgga agatcatgga tttgtggagc ccagcagctg gaccgatgag tataagcgga
     4081 tattccggtt atttgagtct gttttaaaaa aataagcgaa aaataagggg ctaaatactt
     4141 aatttaccgc attttacgca ctaattacaa agtcactata tttgtagttc aagattcggt
     4201 tatggctaaa caaattgaag attttgacgc gaacctgtct gg
//
LOCUS       DAOPMW010000287         4239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24598_length_4239_cov_9.827916, whole genome shotgun sequence.
ACCESSION   DAOPMW010000287 DAOPMW010000000
VERSION     DAOPMW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24598_length_4239_cov_9.827916"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLU11_12020"
     CDS             complement(<1..236)
                     /locus_tag="PLU11_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPN59899.1"
                     /translation="MTSSEKLRLDKYLWSIRLFKTRSAASAACESGKVKYDGNAAKPA
                     RSVSVGDEYEVKTEAKRWRIKVTGLLHNRMAYAEA"
     gene            complement(296..496)
                     /locus_tag="PLU11_12025"
     CDS             complement(296..496)
                     /locus_tag="PLU11_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN59900.1"
                     /translation="MKNKVKDLRTEKGMTQQELADAIGVSRQTIFAMETSRYVPSTIL
                     ALKLAMIFRKKVEDLFELEKKD"
     gene            complement(496..942)
                     /locus_tag="PLU11_12030"
     CDS             complement(496..942)
                     /locus_tag="PLU11_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59901.1"
                     /translation="MMKFLLPHRFKKMGMLLLPLGLFLWVGGQSGLFNTLLTSLHFRR
                     PGFQLILITGFFSFLAGIYFLIFSKEKKEDEFIRHIRLESFHFAALFQLLFFVLLFLV
                     MAIFSLEPGGESAFMLLLAGAILLFWLSYIIRFNYILHIKNRVTRE"
     gene            complement(1055..>2389)
                     /locus_tag="PLU11_12035"
     CDS             complement(1055..>2389)
                     /locus_tag="PLU11_12035"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPN59902.1"
                     /translation="KNNERFLNEMLDLLRIPSISAKIENKDDMLKCAEAVKKSLLAAG
                     CDKAEVMPTAGHPVVYGEKIIDPSKPTVLVYGHYDVQPVDPIELWHSGPFEPTIKDGK
                     VFARGSADDKGQFYMHVKALEIMNQTGTMSTNIKFLIEGEEEVGSPNLGPFVAAHKDL
                     LKCDVILISDSALLSMDTPSLDVGMRGLSYIEVEVTGANRDLHSGTYGGAVANPITIL
                     AKMIASCHDENNHITIPGFYDDVLVATPEERALLNKAPYNEKEYMDELGVQELWGEKG
                     YSTYERTGIRPTLELNGIWGGYTGEGAKTVLPSKAFAKISARLVPNQSTHKITDILIN
                     YLKSIAPKSVTVKAELHHGGEPYLTPIDSKGYQAASKAVETTFGKAPIPVRGGGSIPI
                     CTILEKELGVKIVFMGMGLDNDNLHSPNEKYNIENYYKGIETIPYFHKYFAE"
     assembly_gap    2390..2489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2490..2779
                     /locus_tag="PLU11_12040"
     CDS             <2490..2779
                     /locus_tag="PLU11_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59903.1"
                     /translation="ALAAGTLTRKQRRKLRWRLAWEMTKAKMGFRKSKKEKMSVGLII
                     LLVILLAVSFGTAIWLGMLKEFLILIGAFLLFFILLAGAFKREHGHLSSGK"
     gene            complement(2814..3983)
                     /gene="dinB"
                     /locus_tag="PLU11_12045"
     CDS             complement(2814..3983)
                     /gene="dinB"
                     /locus_tag="PLU11_12045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPN59904.1"
                     /translation="MSENKIRHIAHFDLDSFFVSVEIRNNPSLKGKPVLVGGYERGVV
                     AACSYEARKFGIHSAMPMKKAMQLCPQAIITNASRDQYSKYSRWVTDIIASKVPLFEK
                     ASIDEFYIDLTGMNKFFGVSKYAKELRELIMSETGLPISCGLSSARFISKMATNEAKP
                     NGFLEIPHGKEIEFLWPLGIEKINGVGKQTEQQLKNFGIYTIEDIAKTPVEVMERIGG
                     KWGESLWHKAHGRGSADITTDWEQKSMSHENTFDTDYTDLSFLHKELVRLTEKTAYSL
                     REDEKMTGCVTVKIRYSDFETTSCQETIDYTALDDTLIAKVKDLFTKNWLKGRPVRLL
                     GVRFSHLIPITMQMSLFDNNAGKLNLYKAVDEIKDRFGNKLVTKAVTIKKEKKGN"
     gene            4007..>4239
                     /locus_tag="PLU11_12050"
     CDS             4007..>4239
                     /locus_tag="PLU11_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59905.1"
                     /translation="MNLEWLKTGMGPLKDLLSFLNSEGKTTDVLKKQVIRELRNNLNI
                     FHNAYLNKVSPDIMIELLSNEAIRKAIEENFKF"
BASE COUNT         1072 a          978 c          928 g         1161 t
ORIGIN      
        1 gcttccgcat aggccatccg gttatgcagc aagcccgtca ctttgatccg ccagcgtttg
       61 gcttcggtct ttacctcata ttcatccccc acgcttacac tcctggccgg tttggcggca
      121 tttccatcat atttcacttt gccactctcg caggcagcgg aggcggcgct tctggttttg
      181 aagaggcgga tggaccagag gtatttgtcg agacggagtt tttcggaaga agtcaaaagt
      241 caaaattaaa aagtaaaaaa atgaacagtc agtaaagcgg gactcgtttc acggttcagt
      301 cctttttttc caactcaaat aaatcctcca cttttttcct gaagatcatg gccagtttta
      361 atgccaggat cgtagacgga acatagcggg atgtttccat ggcaaagata gtttgcctgg
      421 acacaccaat ggcgtcggcc agttcctgtt gggtcattcc cttctctgtt cgcaaatctt
      481 ttaccttgtt tttcattact cccgggtaac cctgttttta atatgaagaa tatagttgaa
      541 ccgtataata taggacagcc agaaaagtaa tatagcaccc gccagcaaaa gcataaatgc
      601 cgattccccg ccgggctcaa gggagaagat ggccatcact aaaaaaagca ggacaaagaa
      661 taacagctgg aaaagggctg cgaaatgaaa actttccagc cggatatgtc gaataaactc
      721 atcttccttt ttttctttgg agaatatcag gaagtaaatg ccagccagga atgaaaagaa
      781 tccggtaatc aggatcagct gaaagccagg ccgcctgaag tgcagagagg ttaatagcgt
      841 attaaataat cctgactgcc cgccaaccca aagaaacaat cccagcggga gaagcaacat
      901 ccccattttt ttaaaccggt gaggtaaaag gaatttcatc ataaagttct tatttaattt
      961 actaatgtaa agtttacttt acactcttcc aaataaaaaa aagtcaaaaa gggaagactg
     1021 gtcccatttt gacttttgac tttttacttt ccccctactc cgcgaagtac ttatggaaat
     1081 aagggatcgt ttcaattccc ttataataat tctcaatatt atacttctca ttcggactgt
     1141 gcaggttgtc attatccagt cccatgccca tgaacacgat cttcacgccc agttcttttt
     1201 caagaatggt gcaaatggga atactgccgc cgccacgaac agggatcggg gctttaccaa
     1261 acgtggtttc tactgccttg gaagcagcct ggtagccttt gctgtcaatg ggggtcaggt
     1321 aaggttcacc accatgatgc agttcagctt tcaccgtaac tgatttcggg gcgatggact
     1381 tcaggtaatt gatcaggata tccgtgatct tatgcgtaga ctggttaggc accagccggg
     1441 cggaaatttt ggcaaaggct ttagaaggca atactgtctt tgctccttcg ccggtataac
     1501 ctccccagat accattcagt tcgagggtag gacggatacc ggttctctca taggtggaat
     1561 agcctttctc tccccacaat tcctgtacac ccagctcgtc catgtattcc ttttcgttgt
     1621 aaggggcttt attcagcaaa gctctttctt caggtgtcgc taccagcaca tcatcataaa
     1681 atccgggtat ggtgatgtgg ttgttttcat catgacagct ggcaatcatt ttggccagta
     1741 tggtgatggg gttggctacc gcaccgccat aggtaccact gtgcaagtca cggttagcgc
     1801 cggttacttc cacttcaata taactcagac cgcgcattcc cacatccagc gaaggcgtat
     1861 ccatactcaa cagggcactg tcactgatca gaatcacatc acatttgagt aagtctttat
     1921 gagcggctac aaagggacca agattgggag atcccacttc ttcttcccct tcgatcagga
     1981 atttgatatt ggtggacatg gtgccggtct ggttcatgat ctccagcgct ttcacatgca
     2041 tgtaaaattg tcctttgtca tcggcagaac cacgggcaaa aaccttcccg tctttaatag
     2101 taggttcaaa ggggccgcta tgccagagtt cgatcggatc cactggttga acatcatagt
     2161 gtccatacac caatacagtg ggtttggaag ggtcaatgat tttttctccg taaacaaccg
     2221 ggtgaccggc tgtaggcatg acttccgctt tatcgcagcc ggcagccagt aaacttttct
     2281 ttacggcttc agcacatttc agcatgtcat ctttgttttc aattttggcg ctgattgacg
     2341 ggatgcggag caggtccagc atttcattca ggaaacgttc attgtttttn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgccctggcg gccggtaccc tgacccgtaa
     2521 gcaacgccgt aagctcaggt ggcggctggc ttgggaaatg acaaaagcca aaatgggttt
     2581 ccggaaaagt aaaaaggaga aaatgagtgt gggcctgatt atcctgctgg tgattctgct
     2641 ggcagtcagt tttggtacag ctatctggct tggcatgctc aaggaattcc tgatcctgat
     2701 cggtgctttt ttattattct ttattctcct ggccggggct ttcaaacggg aacacggaca
     2761 cctgtcttca ggtaaataac tacaaccgga tttgttaatt gaaaacagga atattagttt
     2821 ccttttttct cttttttgat ggtaacggcc ttggtcacca gtttattccc aaaccggtcc
     2881 ttgatctcat ccaccgcttt ataaagatta agctttccgg cattattatc aaacaggctc
     2941 atttgcatgg taatcggaat caggtgactg aaacgtacgc ccagtaaacg gactggcctt
     3001 cctttcagcc agtttttagt aaacaggtct ttcaccttgg cgatcagggt atcgtccagg
     3061 gcggtatagt caatggtttc ctggcaactg gtcgtttcaa aatcggaata gcggattttc
     3121 accgttacac aaccggtcat tttctcatcc tctcttaatg aataagccgt tttttcggtt
     3181 agtcgcacca gttctttatg caggaaggac aggtctgtat agtcggtatc aaacgtattt
     3241 tcatgactca tgctcttctg ctcccagtca gtggtaatgt ctgcactgcc ccgtccatgg
     3301 gctttgtgcc agaggctttc tccccattta cccccgatcc gctccattac ttcaacaggg
     3361 gttttagcaa tgtcttcgat ggtataaatg ccaaagtttt tcaattgctg ctctgtctgc
     3421 ttacccacac cgtttatttt ttcaataccc agtggccaga gaaattcaat ctctttacca
     3481 tgaggaattt ccaggaagcc attgggcttg gcttcattgg tggccatctt gctgataaag
     3541 cgggcggagg aaagtccgca ggaaataggc aggccggtct ccgacatgat cagctcacgg
     3601 agttccttag cgtatttact gaccccgaaa aatttattca taccggtgag gtcaatataa
     3661 aattcatcga tactcgcttt ttcaaataaa gggacttttg atgcaataat atcagtcacc
     3721 cagcgggaat acttactgta ctggtcccgg ctggcattgg tgataatggc ttgcggacaa
     3781 agttgcatgg cttttttcat cggcatggcg gaatggatcc caaacttccg ggcttcatag
     3841 ctgcaggccg ccaccacacc ccgttcatac ccgcccacca gtacgggttt tcccttcagg
     3901 gaaggattgt tccggatctc aacggacacg aaaaaagagt cgaggtcaaa atgggcgata
     3961 tgtcgtattt tgttttcaga cactcaccaa agttacttaa agcaatatga acctggaatg
     4021 gctgaaaacg ggaatggggc cactgaaaga cctgctgtct ttcctgaaca gtgaaggaaa
     4081 gaccacggac gtgctgaaga aacaggtaat ccgtgaatta cggaataacc tgaatatttt
     4141 ccataatgcc tacctcaata aagtttcccc ggatatcatg atcgaactgc tttccaatga
     4201 agcaatccgg aaagccattg aggaaaattt taaattcag
//
LOCUS       DAOPMW010000288         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24727_length_4223_cov_5.353167, whole genome shotgun sequence.
ACCESSION   DAOPMW010000288 DAOPMW010000000
VERSION     DAOPMW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24727_length_4223_cov_5.353167"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLU11_12055"
     CDS             <1..1340
                     /locus_tag="PLU11_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPN59906.1"
                     /translation="PPQGGPRDTIPPVLLKSNPGDSAVNFTGNKIVFTFDEFMDVQDI
                     QGNLLVSPMAKINPVVDFKLREVTVKLKDSLEANTTYTLDFGKALKDITEGNIIKDFR
                     FTFSTGAYIDSLELKGNVLMAETAKPDSTMIVLLYTNKDDSAVVKEKPKYIARLDGKG
                     NFHFKNLPAKTFYIYALKDEGGMRRYMNEKQAFAFSDSAVTMQLKNEPVSLFAYAIKE
                     KQVAATDDFGAPGSKPKPGTATEKRLKFQANITNNQQDLLSDLILNFETPLKKFDSTG
                     LRLYTDSTYQPVTELSMLTDSLARTITLRTTWKENTLYNLILDKEFAEDSSGRKLFKT
                     DTISFITKKKTDYGTLKIRFRKLELEKNPVVQLVNGQGIVSSFPLNETEFNLAIFNPG
                     DYEVRILFDANKNGSWDPGQFFGTRRQPEKVTLIGRKITIKPNWTNEYDIDVQ"
     gene            1377..1994
                     /gene="recR"
                     /locus_tag="PLU11_12060"
     CDS             1377..1994
                     /gene="recR"
                     /locus_tag="PLU11_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPN59907.1"
                     /translation="MQFSSSLLENAVNEFAKLPGIGKKTALRLVLHLLKQDSKEVNQF
                     SEVMSRMRSEIKFCQRCFNVADADICSICANSARKQEIICVVESIRDVIAIESTQQYN
                     GIYHILGGVISPLDGVGPDQLNIDSLVNRVQKEKTEELIFALNPNIQGDTTIYYIQKK
                     LQASAIRITTIARGIAFGGELEYADEMTLARSLQNRLPVEKYMSH"
     gene            2204..3232
                     /locus_tag="PLU11_12065"
     CDS             2204..3232
                     /locus_tag="PLU11_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPN59908.1"
                     /translation="MKTIHDCLQERILVIDGAMGTMIQRHKLTEADYRGERFNDWPSD
                     VKGNNDLLNLTRPDIITGIHKEYLEAGADIIETNTFSSTVIAMADYDMQSLAYELNVA
                     AARCARKAIDEYYAAHPEAAPGEKWVAGAIGPLNKTLSLSPDVNNPGFRAVTFDEVVN
                     AYYEQVKGLVDGGVDLLLVETIFDTLNAKGAIYAIKKYFREHPEHQQLPVMISGTITD
                     ASGRTLSGQTLEAFYTSVMHAKPLSVGLNCALGAKEMRPHIEELSQIAGCFVSAYPNA
                     GLPNTMGEYDENPADTGHYLEDWAKEGFVNIVGGCCGTTPDHIKHIAEHVKTIKPRVL
                     PVVETSLA"
     gene            3328..>4223
                     /locus_tag="PLU11_12070"
     CDS             3328..>4223
                     /locus_tag="PLU11_12070"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPN59909.1"
                     /translation="MQSTTVIRPYLRLAGLEPLVVRPETNFVNVGERTNVTGSKKFAR
                     LIRENKYEEALSVARQQVDNGAQVLDVNMDDALLDGVLAMTTFLNLVQSEPDIAKIPI
                     MIDSSKFEIIEAGLKCVQGKCIVNSISMKEGEAKFIEQAIICQSYGAAVIVMAFDELG
                     QADTELRKVEICHRAYKILTEQVGFDPQDIIFDPNIFAVATGLEEHNNYGVDFIEATR
                     KIKKLMPLTKVSGGVSNLSFSFRGNDHVREAMHSVFLFHAIKAGMDMGIVNAGQLVVY
                     DEIEPMLRDLCEDVILNRNNDN"
BASE COUNT         1220 a         1017 c          997 g          989 t
ORIGIN      
        1 tacccccgca aggaggaccg agagatacga ttcccccggt attactgaaa tccaatcccg
       61 gcgattcggc ggtgaatttc actggcaata aaatcgtctt cacgttcgat gaatttatgg
      121 acgtgcagga tatccagggc aatctgctgg tatcgcccat ggcaaagatc aatccggtag
      181 tggactttaa actacgggaa gtcaccgtga aactcaaaga ttcactggaa gccaacacca
      241 cctataccct tgactttggc aaggcattaa aagatatcac cgaagggaat atcattaagg
      301 atttcaggtt taccttttcc accggtgcct atatcgattc cctggaactg aaaggcaatg
      361 tactcatggc ggaaacggct aagcctgaca gtacgatgat cgtcttgctc tacaccaata
      421 aagatgattc tgccgtggta aaagagaaac ctaagtacat cgcccggctg gatggaaaag
      481 gaaactttca ttttaaaaac ctgccggcca aaacctttta tatctatgcc ctgaaagacg
      541 aaggtggcat gcgccggtat atgaacgaaa agcaggcttt tgccttttcc gattcagcgg
      601 ttactatgca actgaaaaat gagcccgtct ccctttttgc ctatgcaata aaagaaaaac
      661 aggtggccgc gaccgatgat ttcggggcac ccggcagcaa acccaagccg ggcacagcga
      721 ctgaaaagcg gctgaaattc caggccaata tcacgaataa ccaacaggat ctgctaagcg
      781 acctgatcct gaattttgaa acacccctta aaaaatttga cagcacggga cttcgtttat
      841 ataccgacag cacttaccag ccggtgacag aactatccat gctgactgat agcctggccc
      901 gcactatcac cctgcgtacg acctggaaag aaaacacgtt gtataacctg atcctggata
      961 aagaatttgc ggaagacagc agcggccgga aattatttaa gaccgatacg atcagtttta
     1021 taactaagaa aaaaacggat tacggtacat tgaagatccg gttccgcaaa ctggaactgg
     1081 aaaaaaaccc ggttgtacag ttggtcaatg gacaaggcat tgtcagttcc ttcccactga
     1141 acgaaactga atttaacctg gccatattta accctggcga ttatgaggtc cgtattttgt
     1201 ttgatgccaa taagaacggc agttgggatc cgggccagtt cttcggtacc cgccgccagc
     1261 ccgaaaaagt aaccctgatc ggacgcaaga ttaccattaa gccaaactgg acgaatgagt
     1321 atgatattga tgtgcagtag gtcacaactt tcaccccacc cgactatctt tgcgccatgc
     1381 aattttcctc ttccttattg gagaacgcgg tcaatgagtt tgccaaacta cccggtattg
     1441 gtaagaaaac cgcgctgcgg ctggtgcttc acctgttgaa gcaggacagc aaggaagtga
     1501 atcagttcag tgaagtaatg agccggatgc gttcggaaat aaaattctgt cagcgttgtt
     1561 ttaatgtggc cgatgctgat atctgttcta tctgcgccaa ttcagcccgg aagcaggaga
     1621 tcatttgtgt ggtggagagt atccgtgatg tgattgccat cgaaagtacc cagcaataca
     1681 atggcatata tcatatactg ggcggggtga tctccccgct ggatggagta ggccccgatc
     1741 agctgaatat tgactccctg gtaaaccggg tacagaagga aaaaacggaa gaactcatct
     1801 ttgccctgaa ccccaatatc cagggggata ccactatcta ttatatccag aaaaaactgc
     1861 aggcatcggc catacgcatc acgaccattg cccgtggtat cgcctttggc ggggaactgg
     1921 aatatgcgga tgaaatgacc ctggcccgca gccttcagaa ccgcctgccg gtcgaaaaat
     1981 acatgagcca ttaatctgtt taaaaacagg actttataaa gaaaagaagc tgtgcttccc
     2041 cctacttgtt tattaaactt attaaatgta attttgcata tcattcaggc ggatttgttt
     2101 attgttcggc ctgccccgga tcccgggaaa aaaacagaac taataaacgg tacggaaccc
     2161 ccaccagagt ttcccaacgt ttatagttca taaaccatta agcatgaaga caatccatga
     2221 ttgtttacaa gaacgcatat tggtcattga tggcgcgatg gggaccatga tccagcggca
     2281 taaactgact gaagcggatt accggggcga acggttcaac gactggccca gtgatgttaa
     2341 aggaaacaat gacctgttga atctgacccg gccggatatc attaccggta ttcacaaaga
     2401 atacctggaa gccggagccg atattataga aaccaacacg ttcagcagta ccgtgattgc
     2461 tatggctgat tatgacatgc aatccctggc ttacgaactg aacgtggccg cagcccgatg
     2521 tgcccgtaaa gccatcgacg aatattatgc agcgcatccc gaagccgctc ccggtgaaaa
     2581 atgggtggct ggtgctatcg gacccttgaa taaaaccctc tccctttccc ccgatgtaaa
     2641 taatccagga ttccgggccg ttacttttga tgaagttgtc aacgcctatt acgaacaggt
     2701 aaaaggactg gtggatggtg gagttgatct cttattggta gaaaccattt ttgacaccct
     2761 gaatgccaaa ggcgcgattt acgccatcaa aaaatatttc cgtgaacatc cggagcacca
     2821 acaactgccg gtaatgatca gcggaacgat caccgatgct tccggacgta cgctcagcgg
     2881 acaaaccctt gaagccttct atacatcggt aatgcacgct aaacctctga gtgtgggcct
     2941 gaactgcgca ctgggtgcaa aggaaatgcg gccgcatatt gaagagcttt cccagatcgc
     3001 cggttgtttt gtatccgcct atcccaatgc cggtttaccc aataccatgg gagaatatga
     3061 tgaaaacccg gcagataccg gccattacct ggaagattgg gccaaagaag gatttgtgaa
     3121 tatagtaggc ggctgctgtg gcaccacccc tgaccatatc aaacatattg ccgaacatgt
     3181 aaaaaccatt aagccacggg tactgcctgt ggtggaaacc tctttagcat gacgacacct
     3241 caatacctga tactgctctc catccttata ctcgttgtaa ataacttttc catgctccgg
     3301 aagttatttg taaaaaagaa ctcatagatg caatccacta ctgtaatcag accttacctg
     3361 agattagcgg gactcgaacc ccttgttgta cgtccggaaa ccaactttgt gaatgttggg
     3421 gaacgcacca atgtaaccgg ttccaaaaaa tttgcccggc tgatccggga aaataaatac
     3481 gaagaagccc tcagcgtggc gcgccagcag gtagataacg gtgcccaggt gctggatgta
     3541 aacatggatg atgccctgct cgacggggta ctggccatga ccaccttcct caacctggtg
     3601 cagagtgagc ccgatattgc caagattcct atcatgatcg acagctctaa gtttgagatc
     3661 atcgaagccg gactgaaatg tgtacaggga aaatgtattg taaactcgat ctccatgaaa
     3721 gaaggagaag cgaagtttat tgaacaggcc atcatctgtc agagctatgg cgccgccgtg
     3781 attgtaatgg cttttgatga actgggacag gccgatacgg aattacgcaa agtggaaatc
     3841 tgccaccggg cttataaaat cctcacggaa caagtaggct tcgatccgca ggatatcatt
     3901 ttcgatccga atattttcgc ggtagccacc ggactggaag aacacaacaa ctatggcgtg
     3961 gattttatag aagccacccg gaaaataaaa aagctgatgc ccctgaccaa agtgagtggc
     4021 ggcgtcagta atctctcctt ctctttccgg ggcaacgacc acgtaaggga ggccatgcac
     4081 agtgtattcc ttttccatgc catcaaagcc ggtatggata tgggtatcgt gaatgccggt
     4141 cagctggtgg tgtatgatga aatagaaccc atgctccggg acctctgcga agatgtgatc
     4201 ctgaaccgga ataacgacaa taa
//
LOCUS       DAOPMW010000289         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24738_length_4222_cov_9.385169, whole genome shotgun sequence.
ACCESSION   DAOPMW010000289 DAOPMW010000000
VERSION     DAOPMW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24738_length_4222_cov_9.385169"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1368
                     /locus_tag="PLU11_12075"
     CDS             28..1368
                     /locus_tag="PLU11_12075"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HPN59910.1"
                     /translation="MKKRLLFLATVLSTLSIFAQPRIVSGPMLGPVELRDAKIWVEVS
                     PEVKSVALQYNKKGDKIRKTIVYKGALGNAFNPLSLTVGGLDYNTTYEYRLLVDGKLT
                     TRGGQFTTKDLWQWRKPIPEFSFLTGSCSYFNEPEFDRPGTPYGKDASIFNTMAKENA
                     AFMLWLGDAWYTREVDYYSNWGLWYRASHDRAVPALQEFLTSMPHLATWDDHDYGPND
                     IGKNYILKETSRTVFNSYFCNPSAGENGQGIYTMTSWGDADIFLTDNRWWRSADLLPD
                     SVNGKPNPEKKMLGDQQMEWLKNSLLYSTATFKIIVVGSQVLNPVSPFDKWRDFPAEY
                     QELMDFLTDHKINGVLFLTGDRHHSEIIKVERPGTYPLYDITVSPLTSGTHVFGAAEK
                     NNPYRVFGLDQHQNYGRFNFSGPRGSRKLMVEFLGVKGEKLGEWAITENELKNR"
     gene            1378..1857
                     /locus_tag="PLU11_12080"
     CDS             1378..1857
                     /locus_tag="PLU11_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HPN59911.1"
                     /translation="MTIYFGYDKRQVIQALRYHFISRREIRLMLILVNVFALATIIMY
                     AMKKITPMPFFVGSSLWIVLMISFWFILPFMVYRKAVTFKHEFSMTFRDDGFTLAHER
                     GSKFWEWTKLSNYLESPHFFHFYFDSRSFLLVPKNGCKDADEVFELRNLIREKVKKG"
     gene            complement(1861..2289)
                     /locus_tag="PLU11_12085"
     CDS             complement(1861..2289)
                     /locus_tag="PLU11_12085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN59912.1"
                     /translation="MALKIIEYGSPDYQKMIGLRDNVLRKPLGLIFTPGELEDEKENI
                     LIGAFEDEDILGCCMLVEENPKVIRLRQMAVLNNLQGKGVGSAILNFAENIARDRGYK
                     VLSMHARKNAIGFYEKMGYKVSGEEFTEVTIPHYLMEKNL"
     gene            2492..2728
                     /locus_tag="PLU11_12090"
     CDS             2492..2728
                     /locus_tag="PLU11_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPN59913.1"
                     /translation="MSDIASRVKKIIVDKLGVDEAEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAETITTVGQAITYLEEHAK"
     gene            2852..4105
                     /gene="fabF"
                     /locus_tag="PLU11_12095"
     CDS             2852..4105
                     /gene="fabF"
                     /locus_tag="PLU11_12095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPN59914.1"
                     /translation="MELKRVVVTGMGAITPLGNTIEDYWTGLINGVSGADMITQFDAS
                     KFRTKFACEVKNFEPTQFLDKKEARKIDRFTQFALVVSDQAMTDAGLNKENINPDRIG
                     VVFASGIGGLTTFQHEVTDFAKGDGTPRFNPFFIPKMILDIAAGQISMRHNLRGPNFA
                     VVSACASSTNAIMDAFNLIRLGKADIILTGGSEAVISEAGVGGFNAMKAMSERNDDPK
                     TASRPYDKDRDGFVMGEAAGMLVLEDLDHALARGAKIYCEVAGCGATADAYHITAPHP
                     EGLGARNVMLAALAEAGMQPDEIDYINTHGTSTPIGDGAEVKAITAVFGEHAYKLNIS
                     ATKSMTGHCLGAAGVIEAIASIKALIHNIVPPTINHFTDDPELDPKLNFTFNKAQQRP
                     VRAALSNTFGFGGHNACVIVKKYPA"
BASE COUNT         1180 a          997 c          970 g         1075 t
ORIGIN      
        1 ctaaattgcg gctccaaaac tgtgtatatg aaaaaacggc tcctctttct cgccacagtc
       61 ctatcaaccc tcagcatttt tgcacaaccc cgtattgttt ccggacctat gctcggacca
      121 gtggaattgc gggatgcgaa gatttgggtg gaggtttcac cggaagtaaa atcagtcgcc
      181 cttcaataca ataagaaagg agataagatc cgtaagacaa tcgtctataa aggagctctt
      241 ggtaatgcgt ttaaccccct gtccttaacc gttggcggat tagattataa taccacgtac
      301 gagtatcgtt tgcttgttga tggcaagcta acaacacgtg gagggcagtt cacgaccaaa
      361 gacctctggc aatggcgtaa acccattccc gaattcagtt tcctgaccgg ctcctgttct
      421 tactttaatg aacctgaatt tgaccgcccg ggtactccct atggcaaaga cgcttccatc
      481 ttcaatacaa tggccaaaga aaatgctgct ttcatgctct ggctgggcga cgcctggtat
      541 acccgggaag tggactatta cagtaattgg ggactttggt accgggccag tcatgaccgg
      601 gccgtaccag cattacagga attccttaca tccatgccgc acctggccac ctgggatgat
      661 catgattatg gtcccaatga tatcggaaag aattatatcc tgaaagaaac cagtcgtaca
      721 gtatttaaca gctatttctg taatccatcc gccggtgaaa acggacaagg catttataca
      781 atgacctcct ggggagatgc ggatattttc ctgacggata accgctggtg gcggagcgcc
      841 gatctgctgc cggattcagt aaacgggaaa cccaatcctg aaaagaaaat gttgggtgac
      901 caacaaatgg aatggctgaa gaattccctc ctatacagta ctgcgacttt taaaattatc
      961 gtcgtcggca gccaggtact gaacccggtt tccccttttg ataaatggcg tgattttccg
     1021 gctgaatacc aggaactgat ggacttcctg actgatcata aaattaacgg agtgctcttt
     1081 ctgaccggcg accggcatca cagtgaaatc attaaagtgg aacgtcccgg tacatatccg
     1141 ctctatgata ttactgtatc accactgacc tcagggaccc atgtttttgg cgcagccgaa
     1201 aaaaataatc cgtatcgagt attcggactg gatcagcacc agaattatgg acgcttcaat
     1261 ttttctggtc ccagaggcag ccggaaactt atggtggaat ttctcggggt aaagggagag
     1321 aaattggggg aatgggccat cacggaaaat gaattaaaga accgctaata cacaagtatg
     1381 actatctatt tcggatacga caaacgacag gtgatccagg cgctgcgcta tcacttcatc
     1441 agccgcaggg aaatcagatt aatgttgatc ctggtcaatg tatttgcact ggctacgata
     1501 atcatgtatg ccatgaaaaa aatcaccccg atgccctttt ttgtcgggtc ttctttatgg
     1561 atcgtactga tgatcagttt ctggtttatc ctgcctttta tggtttatcg gaaagccgtt
     1621 acatttaaac atgaattcag catgaccttc cgggatgacg ggtttaccct tgcccatgaa
     1681 cgaggttcaa aattctggga atggacaaaa ctgagtaatt acctggaaag cccgcatttt
     1741 tttcacttct attttgacag ccggtcattc ctgctggtgc ccaaaaacgg gtgcaaggac
     1801 gctgacgaag tatttgaact ccgtaacctg atcagggaga aagtaaaaaa gggatagggg
     1861 tcagagattc ttttccatca gataatgtgg aatcgttact tcggtaaatt cttcaccgga
     1921 gactttatac cccatttttt catagaagcc aatggcattc ttacgggcat gcatggagag
     1981 cactttgtat ccacggtcac gggcaatatt ttcggcaaaa ttcaggatag cactgcccac
     2041 cccttttccc tggagattat tgaggaccgc catctgccgg agacggatga ccttaggatt
     2101 ttcctctacc agcatacaac agcccaggat atcctcatcc tcaaaagcac cgatcaggat
     2161 attttccttt tcatcctcca gttcgccggg ggtaaagatc agtcccagcg gtttccggag
     2221 cacattatcc cgtaaaccaa tcattttctg gtagtccggg ctgccatatt cgattatttt
     2281 tagtgccata actgaagttt aaggggagag ggacttcaaa atacaaagat ccggtaatac
     2341 taccaaccgt ttattgtgga aatcaggccc agtctgcgct gccgggccct cattttcatt
     2401 attttaagac aattacggaa aaaagacgct ttcgcacgct tatttattca aaaagtatat
     2461 ttttgcaccc aataaacaaa actttacgaa catgtcagac attgcatcaa gagttaaaaa
     2521 gatcatcgtt gacaaactgg gcgttgacga agcagaagtt acaaacgaag cttcttttac
     2581 caacgatctc ggtgccgatt ctttagacac tgtagaactc attatggaat ttgaaaaaga
     2641 attcaacatt tctattcctg atgagcaagc cgaaacgatc acaactgtag gtcaggctat
     2701 tacttacctg gaagagcacg ccaagtaatg tatcatattt tgcctgcctg acggcaggaa
     2761 ataaatgtat cacccgcctt cttaatcccg tacgcacggg acggaaggcg gttggttttt
     2821 atttttgact aaactgcttt actttcacca tatggaactg aaaagagttg tcgttaccgg
     2881 tatgggtgcc atcaccccat tgggaaatac tattgaagat tactggacag gtctcataaa
     2941 cggtgtctca ggtgccgata tgatcaccca gtttgatgct tcaaaattcc ggacaaagtt
     3001 cgcctgtgag gtaaagaact ttgaacccac ccagttcctg gataaaaaag aagcccgtaa
     3061 gattgaccgc ttcacccagt ttgcactggt ggtaagcgat caggccatga cggatgccgg
     3121 actcaataaa gaaaatatca acccggaccg tatcggggtg gtatttgcca gtggtatcgg
     3181 cggactgacc accttccagc atgaagtgac tgatttcgcc aagggagatg gaacaccccg
     3241 tttcaatcct ttctttattc ccaaaatgat cctggatatt gccgccggac aaatcagtat
     3301 gcggcataac ctgcggggac ccaatttcgc agtcgtcagc gcctgcgcca gcagtaccaa
     3361 cgcgatcatg gatgcattca acctgattcg tctcgggaaa gcagatataa tattaaccgg
     3421 cggaagtgaa gcggtgatca gtgaagccgg tgtaggcgga ttcaatgcta tgaaagcaat
     3481 gagtgaacgc aatgatgatc ccaaaacagc cagccggccc tatgataagg accgtgacgg
     3541 atttgtaatg ggtgaagcag ccggtatgct ggtactggaa gacctggatc atgccctggc
     3601 cagaggcgca aaaatttatt gtgaagtggc cggatgcggc gccaccgccg atgcctacca
     3661 tatcacggct ccccatcccg aaggactcgg tgcccgcaat gtaatgctgg cggccctggc
     3721 tgaagccggt atgcagcccg atgagattga ttatatcaat acgcatggca cgtccactcc
     3781 tataggggat ggagctgaag taaaagccat caccgcagtg tttggagaac atgcctacaa
     3841 actcaatatc agtgctacta agtccatgac cggacattgc ctgggcgccg ccggcgtgat
     3901 cgaagcaata gccagtatca aggcccttat acataatatt gtaccaccaa ctatcaatca
     3961 cttcacggac gacccggaac tggatccgaa acttaatttc acatttaata aagcacagca
     4021 acgacccgtg cgggcagccc tgagcaatac gtttgggttt ggcggacata acgcctgtgt
     4081 gattgttaaa aagtatccgg cctgattcgc caaatattcg tagcttgaat accgtggatt
     4141 tttttaaaaa cttttttacc aaacacgaag gttcctcttt caaaaaagaa ctcaggaata
     4201 tgctgggtta taaacccggc aa
//
LOCUS       DAOPMW010000290         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_24891_length_4206_cov_4.841966, whole genome shotgun sequence.
ACCESSION   DAOPMW010000290 DAOPMW010000000
VERSION     DAOPMW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24891_length_4206_cov_4.841966"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1046
                     /locus_tag="PLU11_12100"
     CDS             21..1046
                     /locus_tag="PLU11_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPN59915.1"
                     /translation="MRNSTLLLVYAAFFFMYFFGFGIIAATNAGVPYPWHNQISPLDF
                     SYYIYTIGTAAAAHLIVFRRYYYVKPRWKLVLGIVLLLLAFILYRYSVEELLFPALLG
                     EGNYNPGTTFRFYFFDNVYYGSIRIFIGFVLFLLDELIRSRIQQAALKEQQQKAELRF
                     LEAQMNPHFLFNSLNNIYSLALDKHPETANAVLKLSDMMRYVTYERGQQASLEKELSY
                     IHTMLSIEQLRHDYKLQYQLISSPEAMACRLSPLLLIPLVENALKHGDLSDPTCPLEI
                     KLSKEGPLLNILIRNKKKEQPHATKGGLGLTNLRKRLALSFRPEEFVFRVEENQQTFT
                     VNLHLPA"
     gene            1049..1732
                     /locus_tag="PLU11_12105"
     CDS             1049..1732
                     /locus_tag="PLU11_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPN59916.1"
                     /translation="MYRCIAIDDEPLALNIMADYIRKTDSLHLVDITTSPQQALQRVL
                     NGEADLIFLDIEMPGLNGIEFMQLAGSQQRFILTTAYPDYALKGYEYNVVDYLLKPIA
                     YDRFLKAVQKLPLTTNHPELKQISFFFVKSEYKLLRIDYDDVLFLESLRDYVAIHTVS
                     GHKILTLQSLSSFEKELPSRLFIRIHKSYIVAVNKLELIAKNRVQIGKQEIPIGDTYR
                     TNLNRLTKQ"
     gene            complement(1810..2916)
                     /locus_tag="PLU11_12110"
     CDS             complement(1810..2916)
                     /locus_tag="PLU11_12110"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HPN59917.1"
                     /translation="MRRLLLLLLIAVCTTALSQDHSHSMGRKIVFPDIPGYKTLKTDL
                     HQHTVFSDGSVWPDIRVMEALLDGLDVISLTEHLEYQPHKDDLPHPDRNRSYNLALAE
                     AKNHNLIIVRGSEITRKMPPGHNNAVFIKDANGLLIKDSVEVFKAAKAQGAFVFWNHP
                     NWTAQRKDGIARLTDMHTMLIKEKLLDGIEVVNDDTYSEEALQIALDNNLTIMGTSDI
                     HKLIDWDFKVNDGGHRPVTLVFAKERTEESVKEALFERRTVVYYKDLLIGREPYLVPL
                     LQASITVKSVSYTGKTDVLKVVLVNGTNSPIILSNKTGFTLHSNYDVVTLQPGENTIE
                     VKTLERLKELTLNFEVLSAVFAPGKHPVVGWKVL"
     gene            3202..3651
                     /locus_tag="PLU11_12115"
     CDS             3202..3651
                     /locus_tag="PLU11_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59918.1"
                     /translation="MKKIIIHSLLVLATAISFSLVSNAQPPAGELPMGPPPEMSNKLV
                     TNEETEISRLQMDWMKKKLKLNKEQQQVVEKITREHAKKILLLKNKEGYIAATDPGKK
                     QADLERDEAMKKILTEKQFSRFNKNKQVLENSFDNISGGGLPPPPGM"
     gene            complement(3839..>4206)
                     /locus_tag="PLU11_12120"
     CDS             complement(3839..>4206)
                     /locus_tag="PLU11_12120"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HPN59919.1"
                     /translation="LSNLSTPHLSAMTKHLFSVLYNLPYELPKEKSSIAMTEEELKQY
                     TGVFDLNADLVVKIYVEDGKLIGAPEGQEPLQLHPEKKDHFFLKEIEAKVRFNRNERG
                     EVESMTLFQNGSDITGKKR"
BASE COUNT         1200 a         1009 c          873 g         1124 t
ORIGIN      
        1 ggcttcagta gatttgatgg atgagaaaca gcacgctcct cctggtctat gccgctttct
       61 ttttcatgta ctttttcgga tttggcatta ttgcggccac caatgccggt gtgccctatc
      121 cctggcataa tcagataagt ccgctggatt tttcctacta catctataca atcggaacgg
      181 ctgccgcagc tcatttaatt gtattcaggc gttattacta tgtaaaaccc cgctggaaac
      241 tggtcctggg tattgtcctc ctgctactgg catttatcct ttaccgttat agtgttgaag
      301 aactgctctt tccggcctta ctgggagaag ggaattataa tccgggtact actttccgtt
      361 tctatttctt tgataatgta tactatggtt ccatccggat ttttatcggc tttgtacttt
      421 tcctgctcga tgaactgatc cgcagccgga tacaacaggc cgctttaaaa gaacagcagc
      481 aaaaagcgga actccgcttc cttgaggcac agatgaatcc ccatttcctg ttcaactccc
      541 tgaacaatat ttattcactc gccctcgata aacacccgga aactgccaac gcagtactca
      601 aactcagcga tatgatgcgc tatgtcacct acgaaagagg gcagcaagcc agcctggaaa
      661 aagaactgag ctatatccat accatgcttt cgattgaaca attacggcat gattataaat
      721 tacagtacca gctcatttca tcccctgaag ccatggcctg ccgcctgtct cccctgctgc
      781 ttataccact ggtggaaaat gcccttaaac atggtgacct gtccgaccct acctgtcccc
      841 tggaaataaa actcagtaaa gagggcccct tactgaatat actgatccgg aataaaaaaa
      901 aggaacagcc ccacgctacc aagggtgggc tgggattaac caatctccgg aaaagactgg
      961 cattaagctt ccggccggaa gaatttgtat tccgggttga agaaaatcaa caaacattta
     1021 cagttaatct tcacttacct gcctgactat gtaccgatgt atcgccatag atgatgaacc
     1081 actcgccctg aatatcatgg cagactatat ccgtaaaacg gattcccttc acctggtcga
     1141 tatcacgacc agcccgcaac aagccctgca acgggtactg aatggcgaag cagacctgat
     1201 cttcctggat attgaaatgc ccggactgaa tgggattgaa tttatgcagc tggcaggatc
     1261 tcagcaacgg tttattctca ctacagcata ccctgattat gcgttgaagg gctatgaata
     1321 caatgtggtg gactacctgc ttaaacccat tgcgtacgat cgctttctga aagcggtgca
     1381 gaaacttcct ttaacaacga atcacccgga gttgaaacag atttcttttt tctttgttaa
     1441 atcagagtat aaattgctgc gaattgatta tgacgacgtg ctctttctcg aatcattaag
     1501 ggactatgta gccatccata ccgtatccgg tcacaaaatt ctgaccctgc aaagcctgag
     1561 ttcattcgaa aaagaacttc cttcccggct ctttatccgc atacataaat cttatatagt
     1621 agcagtaaac aaactcgaac tcattgccaa aaaccgggta caaatcggga aacaggaaat
     1681 tcccataggg gatacatacc ggaccaatct aaacagattg actaaacaat agattcagtt
     1741 gccggatgac agttgccaat tgcctgtaaa caaccccttc taagaaagac accataaaca
     1801 ttataaaccc tataacactt tccaacccac caccggatgt tttcccggcg caaataccgc
     1861 actcagtact tcaaaattca gcgtcagttc tttgagtctt tccagtgttt ttacttcaat
     1921 agtattttcc ccgggttgca gggtaaccac atcataatta ctgtgcaaag tgaatccggt
     1981 tttattactg agtataatgg ggctgtttgt gccgttgacc aacacgacct tcagaacatc
     2041 cgtcttaccg gtatagctga ccgattttac agtaatactg gcttgcagca gcggcaccag
     2101 gtagggttcg cgaccaatca ggaggtcttt ataatacaca acggtacgcc tttcaaagag
     2161 ggcttccttt acactctctt ctgttctttc tttggcaaag accaatgtta ccgggcggtg
     2221 accgccgtca ttaactttaa agtcccagtc aatcagttta tggatatcgc tggtacccat
     2281 aatggtgagg ttattatcca gggcaatctg caaggcttct tctgaataag tgtcatcatt
     2341 cactacttca atgccgtcca gcagtttttc cttaataagc atggtatgca tatcggtaag
     2401 ccgggcaatg ccatctttac gttgggctgt ccagttggga tggttccaga atacaaaagc
     2461 accctgtgct ttggccgctt taaaaacttc aacggagtcc ttgatcagta acccgttggc
     2521 atcctttata aacacagcat tattatgacc gggtggcatt ttccgggtga tttctgatcc
     2581 acggacaata atcaggttgt ggttcttcgc ttcggccagg gccaggttat aagaacggtt
     2641 ccggtcagga tgaggtaaat catccttatg tggctggtac tcgaggtgtt ctgtcaatga
     2701 gatcacatcc aatccgtcca acagggcttc cattacccgg atatccggcc atacactccc
     2761 atccgaaaag acagtgtgct gatgcaggtc cgttttcagg gtcttgtacc cgggaatatc
     2821 gggaaataca atcttccggc ccatggaatg actatggtcc tgggataaag cggttgtaca
     2881 tacggcaatc agtaagagaa gcagcagtcg gcgcatagca ggtgttttat gaaaggtaaa
     2941 tttttcaaag taaaaaagca aaagtaaaaa gtaaaaacca ggaagcaccc tttaccttgc
     3001 taaatctcta ttatcaattt attaaatgtt agaattatgc taagaaaagg aagcttccaa
     3061 aacaataaac aaacatctta agaggggctg ttgactgcct tctgcgaact gttttcaccg
     3121 accaatctgt ccatttcatc agcttttcac tttctgtccg caggatacat gccgatattt
     3181 acttccaata aaaaagcgat catgaaaaaa ataatcatac actccttgct ggtactggca
     3241 acggcaattt ctttttctct cgtcagcaat gcccagccgc cagcaggaga attgcccatg
     3301 gggcctcccc cggaaatgag caacaaactg gtcacgaatg aggaaacaga aatcagccgc
     3361 ctgcagatgg actggatgaa gaaaaagctg aaactgaata aagaacaaca acaggtcgtt
     3421 gaaaaaatca cacgggagca cgcaaaaaaa attcttcttc taaaaaataa ggaaggctat
     3481 atagccgcca ccgatcccgg caaaaaacag gccgacctcg aacgggatga agccatgaaa
     3541 aaaatcctga cggaaaaaca attcagccgc ttcaataaga acaaacaggt gctggagaac
     3601 agttttgata atatcagtgg aggcgggctg ccgcctccac cgggaatgta gggagaagca
     3661 acgaggcacg aggtacaaaa ttggtgagca cctgattcta cctgaatggc tataaaaaaa
     3721 ccatcgatta atcttcttaa gaaaactatt gccagattat ctgacaacta tcttagaaag
     3781 ggctgttgac tgttggctgt tggctgtcga ctgtggactg tggactgctt tcctcttctc
     3841 accttttctt cccggtaatg tcactgccat tctgaaacaa agtcatcgat tccacttccc
     3901 ccctttcatt acggttgaag cggaccttag cctctatctc tttgagaaag aaatgatctt
     3961 ttttctccgg atgcaattgt aaaggttctt gtccttcagg agcaccgatg agtttgccat
     4021 cctccacata gattttcacg accaggtcag catttaagtc aaatacaccg gtgtattgtt
     4081 tcagttcctc ctccgtcatg gcgatggacg acttttcttt cggcaattcg taaggcagat
     4141 tataaagaac ggaaaacaga tgcttagtca tggccgagag atgaggtgta cttaaattac
     4201 tcagca
//
LOCUS       DAOPMW010000291         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25035_length_4190_cov_10.022975, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000291 DAOPMW010000000
VERSION     DAOPMW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25035_length_4190_cov_10.022975"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLU11_12125"
     CDS             complement(<1..581)
                     /locus_tag="PLU11_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59920.1"
                     /translation="MKKVSLVQSAVLFFCGLFLTASVFQCTKTGDVIKNLDRSYAGGA
                     DSTLYASFYESNNVQHTDGTPDVNDVIKFRGVQTILHEYCGSSNCHGGPIKPQFDTYA
                     EIMKFVTAGSPETSKLWEYITTNDFNKAMPPVNSSFELGTTDKAVIYNWIKNGAKERP
                     DLADFRPAAIRIINDGCGSANCHNQATATGGWAR"
     gene            849..1064
                     /locus_tag="PLU11_12130"
     CDS             849..1064
                     /locus_tag="PLU11_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59921.1"
                     /translation="MGNNKNNNDQSRRGFFSSFLTKKETEKIKMLTPDGKLVEVDKAV
                     FDQVTGKKKASKEEILNWMENPSKENN"
     gene            1098..2075
                     /locus_tag="PLU11_12135"
     CDS             1098..2075
                     /locus_tag="PLU11_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPN59922.1"
                     /translation="MKQEEINDQSRRGFLKTAAIATVATATCGSLACSCSSKKAPAGD
                     KVKLLSPNGEIVEIDSAYLNHQEHMSIVSKLEAREGIAGKRFVMVVDLANCKNARSCI
                     SACQKWHYRPKEQEWLTVKLLKDSDKGAPYWFPKQCFHCDNPPCVKVCPVDATFKRQD
                     GLVLIDNDRCIGCKFCMAACPYSTRVFNWDEPELPMDIRDLAYSPETGIPAKVGTVEK
                     CDFCPDRAREGLLPPCVEACPNGVFYFGDENEDTVSNGLDTVSFTKLITDRAGYRYME
                     ELGTKPRVYYLPPKDRSFPVEKGFQDADSSIQERFKEYKDILGKKESAE"
     gene            2114..3448
                     /gene="nrfD"
                     /locus_tag="PLU11_12140"
     CDS             2114..3448
                     /gene="nrfD"
                     /locus_tag="PLU11_12140"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPN59923.1"
                     /translation="MELNTYQKEAFDYQRKNIETYGKRAWGWSIFFVICILVGLYALY
                     LQIAKGHGVTGMRDNVVWGLFIVNFIFFIGLSYAGAIIAGLLHLFKVKWGKPIIRLAQ
                     FMTLISVIVGPIFILLCVGRFDRMHHLFIYPRIQSPLTWDVMAVVTFFAGSVLFLWLA
                     LIKDLSVYRDAKLNIPKWKQKVYKFLALGYRGAASQKRHLIISQNLMAIIMIPLAIMV
                     ASILSWIFGMTLRPGWHSTIFGPYFVLGAVYSGCGLLIVAMWFYRKMYKLDYYFTDKH
                     FKYLGYLMLVLAAGYGYFTFSEYFTDWFASEKWDSELIHKLFSTSEYGWWTLFTNVAG
                     IALPILIVAIPATRKPGPITFAAFMMVIAMWVKRYLIIVPTLETTLLPMQDTRAEYVK
                     YAATWPEWALTIAGVATFLLFFTVMSKFVTVVPVSGLEDTHTEHEKHLLHHE"
     gene            3485..>4190
                     /locus_tag="PLU11_12145"
     CDS             3485..>4190
                     /locus_tag="PLU11_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59924.1"
                     /translation="MKQVKEFISPRSSMRPLCLLAVLLVCLLRPASMLAQTDSGAPAA
                     TDTAAAVAAPEEVELISPAIDLISIQKGDSSVDLKATAKAKVDGKMRKLYGLKAVFYM
                     VTDSAETELGTVVTDRNGFAILNVKAGQVVTNADGTLHFKVAVAGPKNMEAGEGEVTV
                     KRAMLIMTPVKEVGLLSVQLKLVDLSTGEEKPVPETAVGIFVKRSFYPLKLGEGNTDE
                     NGEATVEIPNNLPGDPL"
BASE COUNT         1128 a          961 c          976 g         1125 t
ORIGIN      
        1 cgggcccatc cgccggtggc agtggcctgg ttgtgacagt tggcagaacc gcaaccatca
       61 ttaataatac ggatcgcggc aggtctgaag tctgccagat caggtctttc tttagcgccg
      121 tttttaatcc agttataaat aactgcctta tcggtggttc ccagttcgaa ggatgagtta
      181 accggaggca ttgctttgtt aaaatcattg gttgtgatat actcccagag tttgctggtt
      241 tccggactgc cggctgttac aaacttcatg atctctgcat aggtatcaaa ttggggtttg
      301 atcggaccac catggcagtt ggatgaacca caatactcat gcaggattgt ctgcacaccc
      361 ctgaacttaa taacatcatt tacatcgggt gtaccatctg tatgctgaac attgttgctt
      421 tcataaaatg aagcatacaa cgtggagtcg gccccccctg cataagatct gtccagattt
      481 ttaataacat caccggtctt agtacactga aatacggaag ctgtcagaaa cagtccgcag
      541 aagaaaagta ccgccgattg gactaatgaa acctttttca taaaaatcag tttagattga
      601 gaattgatgc ttttgaaaag aactttccct tttaaatgca tttcaaaatt atctcatgca
      661 atggtgacag tttcatgaca agcagctgac cgtaatatga cttttgtcac atgccttagt
      721 aattgctttc taaaacggac cactgtgacg tttctcactt taatgcttga ctactgtcat
      781 gagattgtca atcagtcaat tttaagttgc actgtctttt aattcgttga attaaaaatt
      841 catttaatat gggcaacaac aaaaacaaca acgaccaatc ccgccggggt tttttctctt
      901 ctttcctcac caaaaaggaa acagaaaaaa ttaaaatgct gacaccggat ggtaaactgg
      961 tggaagtgga taaagccgtg tttgaccagg taaccggtaa gaaaaaagcc agtaaagaag
     1021 agatcttaaa ctggatggag aatccttcca aagagaacaa ctaagcattt ccgaaatcaa
     1081 cttattaata tatactaatg aaacaggaag agataaatga ccaaagccgg cggggttttt
     1141 taaaaactgc cgctattgcc accgttgcca ctgccacctg cggcagcctg gcctgttcct
     1201 gctcttccaa aaaagcaccg gcaggtgata aagtaaaact gctttctccc aatggagaaa
     1261 ttgtagagat tgactccgcc tacctgaatc accaggaaca tatgtccata gtatccaaac
     1321 tcgaagcacg cgaaggaatc gcgggcaaaa ggtttgtaat ggtggttgac ctggccaact
     1381 gtaagaatgc ccgtagctgt atttccgcct gtcagaaatg gcactaccgc ccgaaagaac
     1441 aggagtggct gacggtaaaa ctgctcaaag acagtgataa aggagcccct tactggttcc
     1501 ccaaacaatg tttccattgc gacaacccgc cctgtgtgaa agtttgcccg gtggatgcta
     1561 cgtttaaaag acaggatgga ctggtactga tcgataacga ccgttgtatc ggttgtaaat
     1621 tctgtatggc cgcctgcccc tattctaccc gcgtatttaa ctgggacgaa cctgaactgc
     1681 cgatggatat ccgcgacctg gcctattcac ccgaaaccgg tatccccgcc aaagtgggta
     1741 cggtggaaaa atgtgatttc tgtccggacc gcgcccgtga aggactcctc cctccatgtg
     1801 tggaagcctg tcccaatggt gtattctatt tcggcgatga aaatgaagat accgtcagta
     1861 acggtcttga cactgtcagc tttaccaaac tgatcacgga tcgcgccgga taccgctata
     1921 tggaagaact tgggactaaa ccccgggtat attacctgcc gccgaaggac cgctcattcc
     1981 ctgtagaaaa aggcttccag gatgcggact catccatcca ggaacgcttc aaagaataca
     2041 aggatattct tggtaaaaag gaatcagctg aataagccgg tccattgttc atctatttaa
     2101 tgaaatagta attatggaac tcaacaccta ccaaaaagaa gctttcgatt accaacggaa
     2161 gaatattgaa acatatggca aacgtgcctg gggctggagc attttttttg tcatttgcat
     2221 tttagtaggt ctgtatgccc tttacctgca gattgcaaaa ggacatggcg tgaccggtat
     2281 gcgggataat gtggtatggg ggctttttat tgtcaacttt atcttcttta tcgggctgag
     2341 ttatgccggt gctattattg caggtttgct ccatctgttt aaagtaaaat gggggaaacc
     2401 aatcattcga ctggcccagt ttatgaccct gatctccgtg attgtcggtc cgatcttcat
     2461 cctgctttgc gtgggtcgct ttgaccggat gcatcacctg ttcatttatc cgcggatcca
     2521 gtcgcccctg acctgggacg taatggccgt ggtcactttc tttgcaggca gtgtcctctt
     2581 cctgtggctg gccctgatca aggatctttc cgtttaccgg gatgccaaac tgaacatccc
     2641 taaatggaaa caaaaagtgt ataaattcct ggcccttggt taccgtggag ctgccagtca
     2701 gaaaagacac ctgattattt cccaaaacct gatggcgatc attatgattc cgctggccat
     2761 catggtggct tctattctgt cctggatctt tggtatgacc ctccgtccgg gatggcacag
     2821 taccatcttt ggtccttact tcgtactggg tgcggtttac tccggttgtg gtttactgat
     2881 cgtggccatg tggttctacc gcaaaatgta taaactggat tattatttta ccgataagca
     2941 tttcaagtat ctcggatacc tgatgctggt gctggcagcg ggttacggtt actttacttt
     3001 ctctgaatat tttacggact ggtttgcatc agaaaaatgg gacagtgaac taatccataa
     3061 attattcagt acttcagaat atggctggtg gaccctgttt accaatgttg ccggtattgc
     3121 cttaccgata ctgatcgttg ctattccggc caccagaaaa ccaggaccta tcacgtttgc
     3181 ggcctttatg atggtgatcg cgatgtgggt gaaacgttac ctgatcattg tgcccaccct
     3241 cgaaaccacc ctgttaccca tgcaggatac aagggctgaa tatgtgaagt acgctgctac
     3301 ctggcctgaa tgggctttga ctattgccgg tgttgccact ttccttttat tctttaccgt
     3361 gatgtccaag tttgtaaccg ttgtacctgt gtccgggctg gaagacaccc ataccgaaca
     3421 cgaaaaacac ttgctgcatc acgaataagt taattgatta tagaccctgc ttaaataaaa
     3481 agatatgaaa caagtaaaag aatttatatc cccccgcagc tcaatgaggc ctttgtgcct
     3541 gctggctgtg ttgctggttt gtctgcttcg gccagcatcg atgctggcac aaactgacag
     3601 cggagccccg gctgcgaccg ataccgcagc ggctgtggct gccccggagg aagtggaact
     3661 gatttcacct gccattgatt taatcagtat ccagaaagga gatagttctg tagacctgaa
     3721 agccactgcc aaagctaaag tggatgggaa aatgcggaaa ctctatggat taaaagccgt
     3781 gttttatatg gtgaccgact ccgcggagac tgaattaggg accgtggtca ccgaccgcaa
     3841 tggatttgcc attctgaatg ttaaggccgg acaggtagtc accaatgctg atggcacgct
     3901 gcatttcaaa gtagctgttg ccggtcctaa aaacatggaa gccggtgagg gagaagtaac
     3961 cgtgaaaaga gccatgctga tcatgacccc ggtaaaagaa gtaggcttgc tttccgtaca
     4021 actgaaactg gtggatctgt ccaccggtga agaaaaacca gttccggaaa cagctgttgg
     4081 aattttcgtg aaaagaagtt tttatccgtt gaaactcgga gaaggcaata ccgatgaaaa
     4141 cggggaagcc accgttgaaa tccccaacaa cctgcctggt gatccgctgg
//
LOCUS       DAOPMW010000292         4178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25142_length_4178_cov_11.073490, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000292 DAOPMW010000000
VERSION     DAOPMW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25142_length_4178_cov_11.073490"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLU11_12150"
     CDS             complement(<1..188)
                     /locus_tag="PLU11_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPN59925.1"
                     /translation="MFSAKNSSPKQIAAFTALVLAIPESIMVYIVEQSWWWAAGSFLA
                     LFIGGYFLIYFMLERFIYR"
     gene            complement(395..1093)
                     /locus_tag="PLU11_12155"
     CDS             complement(395..1093)
                     /locus_tag="PLU11_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59926.1"
                     /translation="MRKQFPTMESNARKVLIADDEPDILEILKYNLTNEGYEVITAKD
                     GDEALEKARRHSPDLVVLDVMMPKKTGVEVCELLRAQAAFKETLIIFLTAVNDEGTQI
                     RGLETGADDYVSKPISPKVFISRVNALFRRLNKTEVRVLNIGNMTVDPERFIVKVEEQ
                     EIILAKKEFELLYLLALKPGRVFLRNEILNTVWGNDVIVGDRTIDVHIRKIRQKLGID
                     CITTVKGVGYKFEL"
     gene            1293..3551
                     /locus_tag="PLU11_12160"
     CDS             1293..3551
                     /locus_tag="PLU11_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59927.1"
                     /translation="MIKISRYIIFSGLLLVFTCATAQGFAGFDSLSIPAGYKPEFRRQ
                     LNHDGIDGEQKTIMDGDGREDNQFTPSDKEEINQLLTRSLVRKTDWLQFRIETDSTLD
                     HRLKVFYLSGLENLLKYFRQNWKQTGDRNVNPLNFSLIIDAYETCMKTDRKGATIEYI
                     IYSLPYDAGTNLINATIFNKNPGYQPARNHLVLKLCRMHPDRVFTILQQNPDVPFADS
                     LLRALDKRKFARQFYDYAAAANRLGVLIRNIQDDIFIKTIATMAQSRSGQQYFPFLDN
                     IVSGRMTLREIDAAKDDSLLYFRLLVKTQQDYIARAINKDTAFEFKALSRRLEDKAKA
                     DFVNVINGLHNERNLDIRFKSIQQMNAQELYYLAVSSDGSIYTSSFVKGVFPLMMKQS
                     NNRGDSLLMLVNFDKYRKFIKMSAGFNTLDQFLASFPKASAEGEEDPANTLMRAFVNR
                     LEQSDGLEDGVDVADSYASITETLKPVADRMLLNIQDNYERNLGTGNPKGIAIYNILG
                     KLFLSADSTRNIDLTKELGIPPVYEVPFTTLNGDSNRVIVQLFIYGDKDGIGVFPGLI
                     SMFNNPNWKIDQSNKQWVTVSSAKGKPVSLYMNRPLPEETNEDAKAQEALCKYLEDKK
                     LIPTVTINRGHSYNAPYTIDQMSTASKIVFMGSCGGYRMIHDILQKAPDAHIIGTKQI
                     ADAPVNNPFLRLIMEKLRTGSDIKWIPFWEELDKIVTDKIFEDYVPPHKNLGALFIKA
                     YTKAMGPEAINQ"
     gene            3607..>4178
                     /locus_tag="PLU11_12165"
     CDS             3607..>4178
                     /locus_tag="PLU11_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPN59928.1"
                     /translation="MAEPKKTFFDFALLKRVFRYAAPYKRQFYLSVVLAILLALITPV
                     RPLLIKVTVNEYISNEMAAMLINITLIQIGLILVETAMRFLFSYTTALLGQSVVKDMR
                     VATYKKIMHLNLSQFDKTPIGTLTTRTINDIESINDIFSDGLVPIIADLLSIACVLTY
                     MFISDWQLTLIALIPFPIMIIGTYYFKESI"
BASE COUNT         1105 a         1046 c          933 g         1094 t
ORIGIN      
        1 cggtaaataa accgttccag cataaaatag atcaggaaat acccgccgat aaacaaggcc
       61 aggaaagaac ccgccgccca ccaccaggat tgctccacga tatagaccat gatgctctcc
      121 gggatagcca gtaccagggc tgtaaatgca gcgatttgtt tgggggaact gtttttggca
      181 ctgaacatgg cgtaaaatta cggaatacga agtactaagg agaaagtacg aagtacgagg
      241 tacgaagtac gggtttgcta aagcagaaag aggtcttatt tgcagtctgg cgtaattggt
      301 ccttcgaacc tttagttagc tatcggactt taagtttcgt acttcgtacc tcgtacttgg
      361 tttcgtactt aaaactattc ctccgtgata aaactcaaag ctcaaatttg taaccgacgc
      421 ccttaactgt tgtaatgcaa tcaatcccca gcttctgcct gatcttccgg atatgcacat
      481 caatcgtgcg gtcgccgacg atcacgtcgt tgccccatac ggtattgagg atctcgtttc
      541 ggagaaagac cctgcctggt ttgagggcca gcaggtaaag cagttcaaat tcttttttcg
      601 ccaggatgat ctcctgttcc tccactttta caataaaccg ctccgggtca acagtcatat
      661 tgccgatatt caggacccgg acctcggtct tgttcaatcg ccggaacagg gcattgaccc
      721 ggctgataaa tactttggga ctgatgggtt tgctgacata atcgtccgcg ccggtctcga
      781 gtccgcggat ctgggtgcct tcgtcgttta cagccgtgag gaagatgatc agggtttctt
      841 taaaagcggc ctgggcgcga agcagttcac agacctccac cccggtcttt tttggcatca
      901 tcacatccag taccaccagg tcgggactat gccgccgggc tttttccagg gcctcatctc
      961 cgtctttggc ggtaatcacc tcatatcctt cattcgtcag gttatacttg aggatctcga
     1021 gaatgtccgg ttcgtcatcc gcaatcaata ctttccgggc gttggattcc atggtgggaa
     1081 attgtttgcg caaacctaaa gtttaattcc attgcccgga aatttaaccc cgctgcttta
     1141 cacagactta atgataagtt aataccgctt aaacttcctt tttttcattt aacagccgcc
     1201 tgacttccat tttcagcttt aaatcgtctc tatctgttat aaatcaggca tgaatctttc
     1261 cctaaggctt agtaacttta cttttcgatt ctatgattaa aatcagcaga tacattattt
     1321 tctccggact cttactggtc tttacctgtg caacagccca gggctttgcc ggctttgact
     1381 ctctttccat tccggccggg tataagccgg agttccggag acagctgaat catgatggta
     1441 ttgatggcga acagaagacg atcatggacg gggatggacg ggaagacaat cagtttacgc
     1501 ccagtgacaa agaagagatt aaccagctgc tgacccgctc cctggtccgt aaaacagact
     1561 ggctgcagtt ccggatcgaa accgattcca cactggacca ccggttgaaa gtgttttacc
     1621 tctccggact ggagaacctg cttaaatatt tccggcaaaa ctggaaacag accggcgacc
     1681 ggaatgtcaa tcccctgaat ttttccctga tcattgatgc ttacgaaacc tgtatgaaaa
     1741 cagatcggaa aggggctacc attgagtata ttatttatag cctgccgtat gatgccggta
     1801 cgaatctgat taatgccact attttcaata aaaatcccgg ttaccagcct gcccgtaatc
     1861 acctggtgtt gaaactttgc agaatgcacc ccgaccgggt ctttaccatt ttacaacaaa
     1921 acccggatgt gccatttgcg gacagcctgc tccgggccct ggataaacgg aaatttgccc
     1981 gccagtttta tgattacgcc gctgcggcca acagactggg cgtcctgatc cgcaatatcc
     2041 aggatgatat ttttattaaa accattgcca ccatggccca gagccgcagt ggtcagcaat
     2101 actttccctt cctcgataat attgtcagtg gtcgtatgac cctgcgcgaa attgatgcgg
     2161 cgaaggatga ctcgttactt tatttccgtt tactggttaa aacacaacag gactatattg
     2221 cccgggctat caataaagac acggcctttg aattcaaagc cctgtcacgc cggctggaag
     2281 acaaagccaa agctgatttt gtaaatgtca tcaacgggct gcacaatgaa cgtaacctgg
     2341 atatccggtt caaaagtatc cagcagatga acgcccagga actctattac ctggccgttt
     2401 cttccgatgg atcaatttat acctccagtt ttgtaaaagg cgtttttcca ctgatgatga
     2461 agcagagcaa taaccgcggg gattcactgc tgatgctggt aaactttgac aaataccgga
     2521 agtttatcaa aatgtctgcc ggtttcaata ccctcgacca gtttctcgct tcatttccaa
     2581 aagcatccgc tgaaggggag gaagatccgg ccaataccct gatgagagcc tttgtaaacc
     2641 ggttggaaca atcagatggt ctggaagacg gggtggatgt agccgattca tatgccagta
     2701 ttacggaaac actaaaaccc gtggccgacc gtatgctgct gaatatccag gataattatg
     2761 aacgtaacct gggtaccggt aatcccaaag gcatcgctat ttataatatt cttggcaaac
     2821 tttttctcag tgcggattcg acccggaata tagacctgac caaagagttg ggtattcccc
     2881 cggtatatga agtgccgttt accacgctta acggcgacag caaccgggta atcgtacagt
     2941 tatttatcta tggcgacaaa gacgggatcg gggtgtttcc cggactgatc agcatgttta
     3001 ataaccccaa ttggaaaatt gatcagagca ataaacaatg ggtgaccgta tcttccgcta
     3061 aaggcaaacc ggtttccctc tatatgaacc gtcccctgcc cgaggagacc aacgaagatg
     3121 cgaaagcaca ggaagcctta tgcaaatacc tggaagataa aaaactgata ccaacggtca
     3181 ccattaaccg cggacacagt tataatgccc cgtataccat tgaccagatg tccaccgctt
     3241 ccaagatcgt attcatggga tcctgcggcg gttaccggat gatccacgat atcctgcaaa
     3301 aagcaccgga tgcacatatc atcggcacca aacagatcgc agacgccccg gtgaataatc
     3361 ctttcctccg gttgatcatg gaaaaactcc ggaccggcag cgatatcaag tggatcccct
     3421 tctgggaaga actggataag atagtgaccg ataaaatctt cgaggattat gtacccccgc
     3481 acaaaaacct gggtgctttg tttatcaaag cctatacaaa agccatgggg ccggaagcca
     3541 tcaatcaata aacggtaacg ggttctgtct gcttctggtt aaatttgcgg tttacaacac
     3601 attattttgg ctgaaccaaa gaaaacattc ttcgactttg cattgctgaa acgggtattc
     3661 cgttatgcag cgccctataa acggcagttt tacctctccg tagtactggc catcctgctg
     3721 gccctgatca ccccggtgcg cccgcttctg atcaaagtga ccgtgaatga gtatatcagc
     3781 aatgaaatgg ccgctatgct catcaatatc accctgatcc agatcggact catcctggtg
     3841 gaaacggcca tgcggtttct tttttcttat actacggccc tgttgggtca atctgtagta
     3901 aaagatatgc gggtggccac ttataaaaag atcatgcatc tcaacctgtc gcaatttgac
     3961 aagaccccga tcggcaccct tactacgcgg accatcaatg atattgaatc catcaatgat
     4021 atcttctcgg atgggctggt acccattatc gcggatctgt tgtccattgc ctgtgtgctg
     4081 acctatatgt ttatcagcga ctggcaactc acgctgattg ccctgatccc tttcccgatc
     4141 atgatcatcg ggacttatta tttcaaagag agtatcaa
//
LOCUS       DAOPMW010000293         4169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25212_length_4169_cov_6.783179, whole genome shotgun sequence.
ACCESSION   DAOPMW010000293 DAOPMW010000000
VERSION     DAOPMW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25212_length_4169_cov_6.783179"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLU11_12170"
     CDS             <1..200
                     /locus_tag="PLU11_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59929.1"
                     /translation="DSKELRIYSTAIPTSLKLYTLMHNPQYRLSIAWQNVAYNQPPHV
                     SYFLGQGMKMPELPSITVIKK"
     gene            204..974
                     /locus_tag="PLU11_12175"
     CDS             204..974
                     /locus_tag="PLU11_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HPN59930.1"
                     /translation="MKNRLLLLSVILITASAFIFGPQRKPVLYIIGDSTVRNTNRPQC
                     GWGEMIGELLDTAKIKVSNQAMAGRSTRTFIKEKRWEKVINAIQPGDYLIMQFGHNEG
                     SKPDTSRGGYRGVLRGTGNDSVSLNWGDTLTEVVHSYGWYLRKFVREAKAKGGIPVIC
                     SMIPRNQFVEGKVKRADKDFGKWAEEIAKEEGVFFINLNSITADKYDTMGPEQVKTFF
                     PGDHTHTNEEGARVNAASVAEGIRLIKALTLNQYLKNN"
     gene            977..1768
                     /locus_tag="PLU11_12180"
     CDS             977..1768
                     /locus_tag="PLU11_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HPN59931.1"
                     /translation="MKKTGLIYRLLVPVLAILLLSFVWKPQEKPVFYIIGDSTVRNGD
                     GTGKGSLWGWGSFIADQVDTNRLQVQNHAMGGRSSRTFQTEGRWDKIMKTLKKGDYVI
                     MQFGHNDASPLDDTARARGVLRGISDKDTLIYNPIRKMQETVNTYGWYMRKFVKDTKS
                     KGATPIICSLVPRNSWKEGKVQRSGDSWAGWAKQVAAEEGAFFIDLNELVAAEYDQLG
                     AEKIKALYFPSDNTHTNLEGAKINAGYVIDQLRKQNPAMISQYLK"
     gene            complement(1769..2800)
                     /locus_tag="PLU11_12185"
     CDS             complement(1769..2800)
                     /locus_tag="PLU11_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPN59932.1"
                     /translation="MKKNVLFDYIHIDYLSATPKYMQLANSVIKAISEEKITKDDLLP
                     SINELSFEFEISRDTVEKGYKYLKQIGVLGSVPGKGYFVKSTEVEQPLRIFLLFNKLG
                     AHKKIVYDAFVKALGDLALIDFYIYNNDFSLFKKLIQNHKKEYTHYVIIPHFLEGGEG
                     AYDIINQLPSEQLLIMDKEISGIKGNYAAVYENFEKDIYAALEELKEPLSKYHTIKII
                     FPDDTYHPEEILKGFTKFCRQYAFNYRVVHDISKEPIKEGEVFINLMEEDLVTLIEKI
                     LVTKMKVGKHVGVISYNETPLKKIILNGITTISTDFEFMGKKAAEMILNGSTEHVEIP
                     FYVTNRSSL"
     gene            3015..4157
                     /gene="rhaT"
                     /locus_tag="PLU11_12190"
     CDS             3015..4157
                     /gene="rhaT"
                     /locus_tag="PLU11_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HPN59933.1"
                     /translation="MNVILGVIFHFLGGFASGSFYIPYKKVRGWSWESYWIVGGLFSW
                     LVVPPIAAWLTIPGFTEMISGAASSTLGYTYLFGVLWGIGGLTYGLGVRYLGVSLGSS
                     IILGLCMVFGALIPAIYYDFVPTAGKDTFSEMVSSQWGLTIMAGLLLCVVGIVISGKA
                     GMMKEKQLCGAPADPHGTEVKMEYKFGLGMFVSIVSGVLSACFNFGLEAGQELATVAN
                     DTWKAANPGEGEFLYRNNVIYVVLLWGGLTTNFIWCMVLNARNKSFGDYTNKKTPLAR
                     NYLFSAIAGTAWFLQFFFYGMGESKLGNGPSSWILHMAFIILVANMWGLVLKEWKGVS
                     KQTKTVIAVGIATIILSVLLVGRGNSLHEKSKNEKEITYVSSNSKL"
BASE COUNT         1121 a          889 c         1019 g         1140 t
ORIGIN      
        1 atgatagtaa agaactgcgg atatacagta ccgcgattcc tacttcttta aaattatata
       61 ccctcatgca taatccccag taccgcttga gtattgcctg gcagaatgtg gcctataatc
      121 aaccgccgca tgtgagttat ttcctgggac aggggatgaa gatgcccgaa ctaccatcga
      181 tcacggtcat taaaaaataa cgaatgaaga atcgtctgct cctcctttca gtgatactca
      241 taacggcgag tgcatttata ttcgggccgc agcggaaacc ggtactttat attatcggcg
      301 attccactgt taggaacacc aaccgtccgc aatgcggatg gggtgaaatg atcggggagt
      361 tgctggatac ggcaaagatc aaagtgagca accaggccat ggccggtcgc agtacccgga
      421 cttttatcaa agagaaaagg tgggagaaag tcatcaatgc cattcagccc ggtgattacc
      481 tcatcatgca gtttggtcat aatgaaggca gtaaacccga tacttccagg ggcggatatc
      541 ggggtgtact cagaggcacc ggtaatgata gtgtgtcact caactggggc gatacgctga
      601 cggaagtggt acacagttat ggctggtatc tccgcaaatt tgtccgcgaa gcaaaagcca
      661 aaggaggcat tcctgtgatc tgttccatga tcccccgtaa tcagtttgtg gaaggtaaag
      721 tgaaaagagc ggataaggat tttggcaaat gggccgaaga gattgccaaa gaagaaggcg
      781 tatttttcat aaacctgaat agtattaccg ctgataaata cgatacgatg ggaccggaac
      841 aggtgaaaac attttttccg ggtgatcata cccataccaa tgaagaaggt gcccgggtca
      901 atgcggcttc cgtggccgaa ggcatccgtt taataaaagc attgactttg aatcaatacc
      961 tgaaaaataa ctgatcatga aaaagaccgg actgatttac agactcctcg tacctgtgtt
     1021 agctatcctg ttgttgtcat ttgtatggaa accacaggag aaaccggtgt tctatatcat
     1081 tggcgacagc accgtccgca atggagatgg aacgggtaaa ggcagcttat ggggatgggg
     1141 cagctttatt gccgaccagg ttgatacgaa caggctgcag gtacagaatc atgccatggg
     1201 tggacgcagc agccgtacat ttcaaacaga aggccgctgg gataagatca tgaagaccct
     1261 gaagaaagga gattatgtca ttatgcagtt tgggcacaat gatgccagtc cgctggatga
     1321 tactgcccgg gcaaggggtg tacttcgtgg catcagtgat aaggataccc ttatttataa
     1381 tcccatccgg aaaatgcagg aaaccgtaaa tacctacggc tggtatatgc gcaaatttgt
     1441 aaaggatacc aaatccaaag gcgctactcc cattatttgt tcactggtac cccgcaacag
     1501 ctggaaggaa ggaaaagtgc aacgttccgg ggattcctgg gccggatggg caaagcaggt
     1561 ggcagcagag gaaggggctt tctttattga cctgaatgaa ttggtcgcgg cggaatatga
     1621 tcaactcgga gcagagaaaa ttaaggcact ttattttcct tctgacaata cccataccaa
     1681 tctggagggc gcgaagatca atgccggtta cgtaatcgat cagctcagga aacaaaaccc
     1741 ggctatgatc agtcagtacc tgaagtgatt ataacgaaga ccggttcgtg acatagaagg
     1801 gaatctccac atgttcggtg gaaccgttca ggatcatttc ggcagctttc tttcccatga
     1861 attcaaaatc agtggagata gtggtaatgc cgttcaggat aattttcttt aacggggttt
     1921 cattatagga gatcaccccc acgtgtttac ctactttcat tttggtaacc agtatctttt
     1981 cgatcagggt cacgagatcc tcttccatga gattgataaa gacttctccc tccttgatcg
     2041 gttctttgga gatatcatgc accacccggt aattaaaggc gtactgacga cagaatttgg
     2101 taaatccttt cagaatctct tcagggtgat aggtgtcgtc cgggaaaata atcttgatgg
     2161 tatggtattt actcaacggc tcttttaatt cctccagtgc cgcataaata tccttttcaa
     2221 aattctcata taccgcggca tagtttcctt tgatgccgga aatttctttg tccataatca
     2281 gcaactgctc agaggggagc tggttgatga tatcataagc accttcacct ccttcgagga
     2341 aatgcgggat gatcacgtag tgggtgtatt cttttttatg attctggatc agctttttaa
     2401 aaagcgagaa gtcgttatta taaatataga aatcgatcag ggccagatcg ccgagggcct
     2461 tcacaaaagc atcatagacg atttttttat gggcgcccag tttgttaaac agcaggaaaa
     2521 tccggagcgg ttgttccacc tctgtagact tcacaaaata gcccttgccg ggaaccgatc
     2581 ccagcacccc gatttgtttc aggtatttat atcctttttc taccgtatcc ctggaaattt
     2641 cgaattcaaa actcagttca ttaatggagg gcaggaggtc atctttcgtg attttttctt
     2701 cactgatggc cttaatgacg gaattggcca gctgcatgta tttgggtgtg gccgatagat
     2761 agtcaatatg tatgtaatca aataatacgt tttttttcat aacccattcc ttttaaacag
     2821 tttccggggg ctgggaccaa gtccggaacc tgtaagcagt cgtacgtttt aaaagtatat
     2881 atatttcctg atttggccct ttttaatagc ttcaggacat aaaataaagg catgacaggc
     2941 catgacggga gaggaatact attccttagt tttatgatgc gtaaactgat tgcttccaac
     3001 ttgaattccc ctttatgaat gttattttag gtgttatctt tcactttttg ggcggttttg
     3061 cctcgggtag tttttacatc ccttataaga aggtaagagg ctggagctgg gagagttact
     3121 ggattgtcgg tggcttgttt tcctggttag ttgtcccccc gattgccgcc tggctcacta
     3181 ttcctggttt tacagaaatg atcagcggag cggccagttc cacccttggt tatacctatc
     3241 tttttggcgt gctttggggc attggcggac tgacctatgg actgggtgtc cggtatctcg
     3301 gtgtctccct tggaagcagc attatcctcg gtctctgtat ggttttcggc gccctgattc
     3361 ccgctatata ttatgatttt gtgcctaccg caggtaaaga tacattcagt gaaatggtca
     3421 gttcccagtg gggccttacc attatggccg gtctgttgct ttgtgtagtg ggcatcgtca
     3481 tctccggaaa agccggtatg atgaaggaaa aacaactttg tggcgccccg gccgacccac
     3541 atggcacgga agtgaaaatg gaatataaat tcggtctggg tatgttcgta tccattgtat
     3601 cgggtgtact cagtgcctgt ttcaatttcg gtctggaagc cggacaggaa ctggccaccg
     3661 tagccaatga tacctggaaa gcagccaatc ccggtgaagg ggagttttta taccggaaca
     3721 atgttatata tgttgtcctg ttatggggcg gactcaccac caactttatc tggtgtatgg
     3781 tgctgaatgc ccggaacaaa tccttcggtg attataccaa taaaaagact ccgcttgccc
     3841 gcaactattt attctccgcc attgctggta cggcctggtt cctccagttc ttcttctatg
     3901 gcatgggtga aagcaaactc ggcaacggcc ccagctcctg gatactgcac atggctttta
     3961 ttatattagt agccaatatg tggggactgg tgctgaaaga atggaaagga gtgagcaaac
     4021 aaacaaagac agtcatcgca gtcggtatcg cgaccatcat tctctctgta ttactggtgg
     4081 gaagaggcaa cagtctgcat gaaaaatcga aaaacgaaaa agaaattaca tatgtcagta
     4141 gcaacagcaa actttaagca tgtaagtta
//
LOCUS       DAOPMW010000294         4165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25243_length_4165_cov_4.977859, whole genome shotgun sequence.
ACCESSION   DAOPMW010000294 DAOPMW010000000
VERSION     DAOPMW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25243_length_4165_cov_4.977859"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLU11_12195"
     CDS             complement(<1..205)
                     /locus_tag="PLU11_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HPN59934.1"
                     /translation="METFETIKSETPLWDKGKLFIKSIVIFGIALFLWVPTFFIMGMV
                     KEREGRQKEAILDISSKWAGKQTV"
     gene            complement(245..721)
                     /locus_tag="PLU11_12200"
     CDS             complement(245..721)
                     /locus_tag="PLU11_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59935.1"
                     /translation="MNNLIIIRRWIIFFIVCLVVSGATAIPAESELQLATRLIPRGTA
                     IGDWLDKVYMSVVHQNQQAPFLAYGYDWLAFAHFALAILFIGPLKDPVKNTWVIEFGM
                     IACALVIPFALVAGYMRGLPLWWRLVDCSFGVIGFIPLLIVRRKIDLLEAEEECSI"
     gene            complement(767..1831)
                     /locus_tag="PLU11_12205"
     CDS             complement(767..1831)
                     /locus_tag="PLU11_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59936.1"
                     /translation="MFIVIVFFFGSVGLYALADLISVINNNERAGGAFFFRSFEFSVV
                     ILLPLLFLSLFDIGEKNDCCSDSAFFSPAHRLSVYVLILLCSAAYYYSSLRKRTAPPL
                     IELAVNALLSVALILCVLIAMQARETLMWIGGIPAIMFFYIGQMIINHRMLLVETEDQ
                     LTPVQEPYSKAILLILKSNVFIKFPLLLVISVPLLLFLSLVLYVFGQRPDSAIRAFTE
                     TYKHGFSQWDYQCENVECGGHYLCSVAANGHPSVVKPVRIGKRHGHYIICNRQLLVSN
                     AFEELIQEKWPALHRIIRKQYDKVGDMVHRNYNVYSKKWVADMVYVIMKPAEWAFLLV
                     LYLADRKPEQRIARQYAPWD"
     gene            complement(1839..2138)
                     /locus_tag="PLU11_12210"
     CDS             complement(1839..2138)
                     /locus_tag="PLU11_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPN59937.1"
                     /translation="MKNPITGLNKVFDNRIRLGVMSVLMVNEEISFNDLKQLLEVTDG
                     NLATHLVNLEENGFIKVHKGFIGRKTNTTYSITRQGEKAFSDHISALENMIKGVK"
     gene            complement(2156..2800)
                     /locus_tag="PLU11_12215"
     CDS             complement(2156..2800)
                     /locus_tag="PLU11_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59938.1"
                     /translation="MEEKLPGTNSLNDLNDIRRIMERSSRFVSLSGLSGVAAGICAIG
                     GAFIANSYFSNYYGVYNSRGFTTGDEFSQLKLRLILLGGVVFLMAFASSYYLTWRKAR
                     KAGLPVWDHTSKRLAWNMMVPLMAGGAFIIGMLRYDVWIFVAPACLVFYGLALVNASK
                     YTLSDIRYLGYCEIVLGTINMLYPGYGLWFWAAGFGVLHIIYGVIMWNKYERNN"
     gene            2928..>4165
                     /locus_tag="PLU11_12220"
     CDS             2928..>4165
                     /locus_tag="PLU11_12220"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPN59939.1"
                     /translation="MKRPKLLVSYVIFLLLSLATIPAGGQTKIIHGLVRDAHSEEPVP
                     FASILFKNSTIGKLTDSSGQFSFYLSKLPSDTLLITCVGYQPFSLILNNLRDSSSFSI
                     LMERGTFNEGAQLKVKVNKGLLVWRKIVQNKPFNDRYRFDNFSYELYNKLELDLKNIN
                     FEKFGRFKPLKPISELINQNIDTSEGIKYLPTYLTEALSDYYYQKSPKKRREIIKAYN
                     TNGVKNESVSKMLGGMDQVVNVYNNFIPVFDKQFVSPISDNGDYYYNYRVVDTQVIAN
                     KRYFHLVFTPRRKGGDTFEGDCWVHVGSFAIQRMNLRLGKEANVNFIENLSLIQEYKQ
                     INDSTWFLSKDKFVADVSPAGKDRPGFIGRKTTTYDHIVVNDSSVLNELSKNKILEEI
                     ITLSEAGKKTKEFWAVERHEP"
BASE COUNT         1261 a         1029 c          838 g         1037 t
ORIGIN      
        1 taacggtttg tttgcctgcc catttactgc tgatatccag gatcgcttct ttctgccggc
       61 cttcccgttc tttcaccatc cccatgatga aaaaagtggg cacccagaga aagagtgcaa
      121 taccgaagat cacgatggat ttgataaata atttcccttt atcccataaa ggggtttctg
      181 atttgatggt ttcgaatgtt tccatgatac tattattaat tgtttgttgt ttgacgtttg
      241 ttgtttagat ggagcattct tcttccgctt ccagcagatc aatcttccgg cgcacaatca
      301 gcagcggaat aaacccgatc accccaaagg agcaatccac caatctccac cagagcggaa
      361 gtccgcgcat ataaccggcc accagggcaa agggaatcac cagggcgcag gcgatcatac
      421 cgaactcgat cacccaggtg ttcttcacgg ggtcctttaa cggacctata aagagaatag
      481 ccaatgcgaa atgggcaaag gccagccaat catagccgta agccagaaag ggggcctgct
      541 gattttgatg taccactgac atgtaaactt tatccagcca gtcgccaata gcggtacctc
      601 tggggatcag cctggtagcc agttgcaatt cactttcagc aggtatggcg gtggccccgc
      661 tgacaacgag acatacaata aaaaagataa tccagcgtct gataataatt aggttattca
      721 taataaaaag cactttgtat ttcaaagtat aaaaacaaaa aaaaactcag tcccagggag
      781 catactgacg ggcgatccgt tgctccggtt tccggtcggc cagatacagt accaacagga
      841 acgcccattc tgcgggtttc atgattacgt ataccatatc agccacccat tttttactgt
      901 acacattata attccggtgt accatatccc ccactttatc gtactgtttc cggatgatcc
      961 ggtgcaaagc cggccatttc tcctggatca attcttcaaa ggcatttgac accagtaatt
     1021 ggcggttaca gataatatag tgtccgtgtc gtttaccaat tcttactggt ttaactacac
     1081 ttggatgccc gttggcggcc accgaacaga gataatgccc gccacattct acattttcgc
     1141 attggtaatc ccattgcgaa aatccatgct tataagtttc cgtaaaggcc cggatggctg
     1201 aatcggggcg ctgaccgaag acatataaaa ccagtgaaag aaataaaagc aatggcacag
     1261 aaattacaag cagtaaaggg aatttgataa aaacattgga ttttaaaatc aacaagatgg
     1321 ctttactata aggctcctgt acaggtgtaa gctgatcttc tgtttcaacc aacagcatac
     1381 gatgattaat aatcatctgg ccaatataaa aaaacatgat tgccggtatt ccgcctatcc
     1441 acataagcgt ttcccttgcc tgcatggcga tcagaacaca taaaataaga gctacactca
     1501 ataatgcatt aacggccagc tcgatcaaag ggggtgcggt gcgcttacgc aacgaagaat
     1561 aataataagc agcgctgcat aataagataa ggacatatac cgataaacgg tgtgccggtg
     1621 aaaaaaaagc ggaatcggaa caacaatcat tcttttcacc tatgtcaaat aaagatagaa
     1681 aaagtagtgg cagaagaata actactgaga attcgaaact cctgaaaaaa aaggcaccgc
     1741 ctgctctttc attattattg atcacactga tcagatccgc cagggcatac aacccgacag
     1801 atccaaagaa aaacacaatt acaataaaca atcccatact atttaactcc tttaatcatg
     1861 ttttccaatg ccgatatatg atccgagaac gctttctccc cctgccgggt aatggaatag
     1921 gtggtattgg ttttccttcc gataaatcct ttatgcactt taataaaccc attctcctcc
     1981 aggttcacca ggtgagtggc caggttgcca tccgtcactt ccagcagctg cttgaggtcg
     2041 ttgaaactga tctcctcatt gaccatgagc acactcatca cccccagccg gatgcggttg
     2101 tcaaaaactt tatttaaacc ggtgatcgga tttttcatgt cgtaaagttc tatgctcagt
     2161 tatttcgttc gtatttattc cacatgatca caccatagat aatatgcagc accccaaaac
     2221 ctgccgccca gaaccataga ccataaccgg gatagagcat attgatggtc ccgagtacaa
     2281 tttcacaata acccaggtaa cggatatcgg aaagggtgta tttactcgca ttcaccaggg
     2341 ccagcccgta aaataccagg caggccggcg ccacaaaaat ccagacatca tagcgcagca
     2401 taccaataat aaaagcacca cccgccatca acggcaccat catattccag gccaggcgct
     2461 tcgatgtatg atcccatacc ggtagtcccg ccttacgggc cttacgccag gttaaataat
     2521 aagaagaagc aaaggccatt agaaaaacta ctccccccaa aaggatcagt cttagtttca
     2581 actgactaaa ctcatccccc gtggtaaatc cccggctgtt gtagacccca taataattgc
     2641 tgaaataact attggctatg aatgcccctc caatggcaca tatgccagcc gccaccccgc
     2701 tcaatccgct gagtgataca aatcgactcg acctttccat gatccggcgg atatcattga
     2761 ggtcatttaa ggagttggtt ccgggtaatt tttcttccat aaaaagcact ttgtaataca
     2821 aagtatgtga aaattcccca accttccaaa ttttttccgg ctctttctgc cctttcaccg
     2881 attttaacat atgactataa ccataggcga tgtattttta cggctctatg aagcgaccga
     2941 aactgttggt atcttatgta atattcctgc tattgtccct ggctactata ccagcaggcg
     3001 gacaaacaaa aatcatccat ggcctggtcc gggatgccca cagcgaagaa ccggtcccct
     3061 ttgcctccat cctttttaaa aacagcacga tcggtaaact aaccgactct tccggccaat
     3121 tcagcttcta cctgtctaaa ctcccctccg ataccctgct gatcacttgt gtgggttacc
     3181 aacccttcag cctgattcta aataacctac gggattcttc ctccttttcc atactgatgg
     3241 aaagaggaac ctttaatgag ggggcccagt tgaaagtaaa ggtgaacaaa gggttattag
     3301 tctggcgtaa aatcgtgcaa aacaaacctt ttaatgaccg atatcggttt gataattttt
     3361 cctacgagtt atacaacaaa ctcgaactgg acctgaagaa catcaatttt gaaaaattcg
     3421 gccggttcaa accactcaaa ccgattagcg aactgatcaa ccagaatatt gacacgtcag
     3481 aaggtatcaa atacctgcct acttacctca ccgaagcgct ctccgattat tactaccaga
     3541 agtccccgaa gaaaagaagg gagatcatca aagcctataa tactaacggg gtaaaaaatg
     3601 aaagtgtctc taaaatgctg ggcggcatgg accaggtagt aaatgtgtac aacaacttta
     3661 tcccggtatt tgacaaacaa ttcgtgagcc cgatcagcga taacggtgac tattattaca
     3721 actaccgggt ggtagatacc caggtaattg ccaataaacg ctatttccac ctggtattca
     3781 ctccccgccg aaaaggtggc gatacttttg aaggcgattg ctgggtacat gtgggcagtt
     3841 ttgccatcca gcggatgaac ctccgcctgg gcaaggaagc caatgtaaat tttattgaaa
     3901 acctgagcct gatccaggaa tacaaacaaa tcaatgacag cacctggttc ctgtcaaagg
     3961 ataagtttgt ggccgatgtg tcccccgccg ggaaagaccg gcccggattt atcgggagaa
     4021 agaccaccac ctatgaccat attgtggtca atgacagcag tgtgctgaat gaactcagca
     4081 aaaacaaaat cctcgaagaa atcatcaccc tgtcagaagc cggtaaaaaa acaaaggagt
     4141 tctgggccgt ggaacgtcat gaacc
//
LOCUS       DAOPMW010000295         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25256_length_4163_cov_8.824489, whole genome shotgun sequence.
ACCESSION   DAOPMW010000295 DAOPMW010000000
VERSION     DAOPMW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25256_length_4163_cov_8.824489"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLU11_12225"
     CDS             complement(<1..809)
                     /locus_tag="PLU11_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPN59940.1"
                     /translation="MKLVTYLKEEREQLGVLINGMLYDMEVLHPELPNSMNMMLNYWE
                     EFFPFAQAGELMLREGTRTSSRGIPISEAQLLAPIPFPTSCRDGYAFRQHVAAARRNR
                     KVDMIPEFDQYPIFYFTNHHSIQGPGDVRCMPDHFEKLDFELEAAIVICKHGRNIRAE
                     EADQYIGGLMIMNDLSARRLQMEEMLLNLGPAKGKDFSTVIGPWLVTLDELEQFEIPA
                     KENHTGKSWNLRMQCKVNGKQVSDGNVGDMDWTFAEIIERASYGVDLYAGDV"
     gene            895..3447
                     /locus_tag="PLU11_12230"
     CDS             895..3447
                     /locus_tag="PLU11_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPN59941.1"
                     /translation="MKKGFFLLLLFPLLLTAQQAPQSWIRINQLGYTPGGLKTAVWVT
                     KEDNGFGKWELVERTTGKVVMIGYAGKDFGAYGPFKHSYRMQFTAFHKPGEYFIRTGE
                     TTSPVFRIGQDVYKGAADFCLRYMRQQRTGFNPFLKDSCHTHDGYVLYGEKAGIKDST
                     HIDVVGGWHDASDYLQYSSTSANATYHLLMAYRDFPKVFTDEKQANGLDGKNGRADVL
                     DEAKWGLDWLLKMHPKDNWLFNQIGDDRDHMGMRIPKEDTNYYGPGLDRPVYFINGEP
                     QQRGKFMNATTGTSSTAGKFSSAFALGSSIFKKTDPVFAARLSKKSFSAYNFGKKKTG
                     FTQTVSVRSPYIYAEMNWSDDMELAAASLYNNTRDVKYQAASFAYAQIEKLTPWLGSD
                     TAAHYQWYPFINAGHNELAKQLASTSRSTITGFYKQGIEKVWSKAQSNAFYRGIPYIW
                     CSNNLTTSFAIQCYWYKQLTGDNQFSELEQANFDWLFGCNPWGTSMVYGLPAWGDTPV
                     DPHSAFTHLKNYPIDGGLVDGPVYGSIFNGLIGIQLKDADEYAAFQSDLAVYHDDYGD
                     YSTNEPTMDGTASLIYLLAAKEAGAEPEPDPPHQLPWESVHPGSMEIKKNFSHDRGAI
                     IRGDSTRKELALVFTGHEFGEGGTFIDSLLLKEKIQASFFLTGDFYRNEQFRPLIKGL
                     RRRGHYLGAHSDKHLLYCDWTKRDSLLLNEQQFSLDLADNYAAMAAHGIEANKSRFFL
                     PPYEWYNDSIADWTSRFGMQLINYTPGTRSTADYTRDADKNYRDNETILNSITGYEGS
                     RPAGLKGFILLMHAGAGPGRTDKFYKKLPELIKYLKTKGYRFVRIDELLQPG"
     gene            3532..>4163
                     /locus_tag="PLU11_12235"
     CDS             3532..>4163
                     /locus_tag="PLU11_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPN59942.1"
                     /translation="MKFEKIHNKGQARLFENDFLEALTKAHPLVIWGMYIPIMVYIIY
                     LAATRYDYGTGTILLTFFGGAFFWSFFEYLAHRFLFHWIPKGKLATKIVYTMHGNHHH
                     YPRDRQRLFMPPVPSLIIATILFSTQWLIMGSYALMFFPGFLIGYLMYGTMHYAIHAW
                     NPPFKWMKPLWRNHHLHHYKSEHQGFGVSSTIWDHVFGTMFDLKEKEDKE"
BASE COUNT         1074 a         1011 c         1012 g         1066 t
ORIGIN      
        1 acatcgccgg catagaggtc cacgccatag gaagcgcgtt cgatgatctc ggcaaaagtc
       61 cagtccatat cgcccacatt gccatcgctc acctgtttgc cattcacctt gcattgcatc
      121 cgcaggttcc agcttttgcc ggtatggttt tccttggctg gaatttcaaa ttgttccagt
      181 tcgtccaggg tcaccagcca gggtccgatc acggtagaga aatcttttcc cttggcggga
      241 ccgaggttca acaacatttc ttccatctgc aggcgccttg cactcaggtc attcatgatc
      301 atcagtccgc caatatactg gtccgcttct tccgcacgga tatttcggcc atgtttgcag
      361 atcacgatgg cggcttccag ctcaaaatcc agtttttcga aatgatcggg catacaacgc
      421 acatcgcccg gcccctggat actatgatga ttggtaaaat aaaaaatcgg gtactggtca
      481 aactcgggga tcatgtctac tttgcggtta cgtcgtgctg ctgccacgtg ctgacgaaag
      541 gcatagccat cccggcagga agtgggaaag gggatggggg ccaataactg cgcttcgctg
      601 atcggaatac cacgactact ggttcgggtg ccttcacgta acatcagttc gccggcctgg
      661 gcaaacggga aaaactcttc ccagtaattc agcatcatgt tcatgctgtt gggcagttcc
      721 ggatgcagga cttccatatc gtagagcatc ccattgatca ggacgcccag ttgttctctt
      781 tcttctttga ggtaagtgac taatttcatc tggttaacat tataagttgc taagttatac
      841 taaatagggt ttccaatccg gcgggtattg accggcagtt tgtaaattgt gggtatgaaa
      901 aaaggcttct ttctgcttct cttgtttccc ctgctgctga cggctcagca agcaccgcaa
      961 agctggatcc ggatcaatca gctgggctat accccgggcg gactgaaaac cgcggtctgg
     1021 gtaaccaaag aggataacgg ttttggtaaa tgggaattgg tggagcgaac caccggcaaa
     1081 gtggtcatga tcgggtatgc cggcaaggac tttggagctt atggtccttt taaacatagt
     1141 taccgaatgc agttcaccgc ctttcataag ccgggagagt acttcatacg gacaggtgaa
     1201 actacctctc ctgttttccg gatcgggcag gatgtatata aaggggcggc cgatttctgc
     1261 ctgcgttata tgcgacaaca acgcaccggc tttaatcctt ttttaaaaga cagttgtcat
     1321 acccacgacg gctatgtatt gtatggcgaa aaagccggga tcaaagacag cacccatatc
     1381 gatgtggtgg gtggctggca tgatgcttcg gattatttac agtatagttc cacgtcggcc
     1441 aatgccacat atcatttatt gatggcttac cgcgatttcc ccaaagtgtt taccgatgaa
     1501 aagcaggcca acggactcga tgggaagaac ggaagggcgg atgtgctgga tgaagcaaaa
     1561 tggggactgg actggttatt gaaaatgcat ccgaaagata actggctgtt caaccagatc
     1621 ggcgacgacc gcgaccatat gggcatgcgg atccccaaag aggatacgaa ttattacgga
     1681 cctggtctgg atcgtccggt gtactttatt aatggcgaac cgcagcagcg gggtaagttc
     1741 atgaatgcca ctacgggtac gagttctaca gccggaaaat tctccagtgc ttttgccctg
     1801 ggttcatcca tatttaaaaa gacggatcct gtatttgctg ccaggctttc aaaaaaatca
     1861 ttctctgcat acaattttgg gaaaaagaaa acaggattta cacaaacggt atctgtacgt
     1921 tctccctata tatatgcgga aatgaactgg tcggatgata tggaactggc agccgcctct
     1981 ttatataaca atacccgtga tgtaaaatac caggcagcgt cctttgctta tgcacaaata
     2041 gaaaagctga caccctggct gggatcagat acagctgcgc actaccaatg gtatccattc
     2101 attaatgccg gacataatga actggcgaaa caattggcaa gtacttccag aagtaccatc
     2161 accggatttt ataagcaggg tattgaaaaa gtctggagta aagcacagtc caatgccttc
     2221 taccgtggca ttccctatat ctggtgcagc aataacctca ctacatcatt tgcgattcag
     2281 tgttattggt acaaacaact cacaggtgat aatcagttca gcgaactcga acaggctaat
     2341 ttcgactggc tatttggctg taatccctgg ggtacttcga tggtctatgg tttaccggcc
     2401 tggggcgata caccggtgga tccgcattca gcctttaccc atttaaaaaa ttatccgatc
     2461 gatggcgggt tggtagacgg tccggtgtat ggcagtattt tcaacggact gatcggtatc
     2521 caactgaagg atgcggatga atatgccgct tttcaaagtg accttgccgt atatcatgat
     2581 gattatggag attatagtac caacgaacct accatggatg gcacggcatc actgatctac
     2641 ctgctggcgg ccaaagaagc cggtgcagaa cccgagcccg atccacccca ccagttaccc
     2701 tgggaaagtg tgcatcccgg ttccatggaa ataaaaaaga acttcagtca tgaccggggt
     2761 gcgatcatcc ggggtgacag tacccggaag gaattagccc tcgtatttac cgggcatgag
     2821 tttggtgaag gcgggacctt tatcgacagc ctgctgttga aagaaaagat tcaggcttcc
     2881 ttcttcctga ccggcgattt ttaccgcaat gagcaattcc gtccgttgat caaaggactc
     2941 cgccgccgcg ggcattacct gggggcccat tcggataaac acctgttgta ctgcgactgg
     3001 acaaaaagag acagtctgct cctgaatgag caacagttca gtctcgacct ggccgataac
     3061 tatgccgcca tggcggcaca tgggattgaa gcgaataaat cccgcttctt cctgccaccc
     3121 tacgaatggt acaatgattc cattgcggac tggaccagcc ggtttgggat gcaactgatc
     3181 aactatacac ccggcacccg gagtacagcc gactatacca gggatgcaga taaaaattac
     3241 cgggacaatg aaaccatcct gaactccatt accggttacg agggcagccg gccggccgga
     3301 ctaaaagggt ttatcctgct gatgcacgcg ggggcaggac ctggtcggac agataaattt
     3361 tataaaaaac tgcctgaatt aataaaatat ttgaaaacaa aaggttaccg gtttgtacgg
     3421 attgatgaat tattgcagcc cggttagata gttttatttg caactatttt tcattgttaa
     3481 aatcggattt tggtcacaat aaatcaaccc agcccgtatt atctttgcaa catgaaattt
     3541 gagaagattc acaataaagg gcaggcccgc ctattcgaaa atgacttcct ggaggcactg
     3601 acgaaggccc atccacttgt gatctggggg atgtatatcc cgatcatggt ttatattatt
     3661 tacctggctg ccaccaggta cgactacggc acgggtacca tcctgctgac cttctttggc
     3721 ggtgcatttt tctggtcttt tttcgaatac ctggcccacc ggttcctctt tcattggata
     3781 ccgaagggaa agttggcgac caagattgtt tataccatgc atggtaatca ccatcactat
     3841 ccccgtgacc ggcaacgttt gtttatgccg cctgttccca gcctgatcat tgccactatc
     3901 ctgtttagta cgcagtggct aattatgggt tcatatgccc tcatgttttt ccccggtttc
     3961 ctcatcggct acctcatgta tggtaccatg cactatgcca tccatgcctg gaacccgccg
     4021 tttaaatgga tgaaaccgct ctggagaaat catcacctgc atcactataa aagcgaacac
     4081 cagggtttcg gcgtcagctc caccatctgg gaccatgtct tcggtaccat gttcgatctc
     4141 aaagagaagg aggataagga gaa
//
LOCUS       DAOPMW010000296         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25320_length_4155_cov_8.502195, whole genome shotgun sequence.
ACCESSION   DAOPMW010000296 DAOPMW010000000
VERSION     DAOPMW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25320_length_4155_cov_8.502195"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLU11_12240"
     CDS             <1..493
                     /locus_tag="PLU11_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59943.1"
                     /translation="LVLFLYSFSSRGMAITAWWYLLTGHIFILAAVLFNAPHIYFSEL
                     FFYASGVLIAFLLGYYCLLKIERIDQDISLSRFHGYVYEQENTGFLFLLAAMGMLGFP
                     ITAAFIGIDVLFTYIHSDQYVLIAMLGLSLFFVELAAFRILLRIFFGPHKKLYHPVAF
                     RST"
     gene            complement(500..1969)
                     /locus_tag="PLU11_12245"
     CDS             complement(500..1969)
                     /locus_tag="PLU11_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN59944.1"
                     /translation="MQTHSKYKIINWLRQRNQQRKKTILITGLMLILLFLAKLSMAQD
                     SLQLQTLSASQVMSIVKRYHPVARIADINIDMAKADITIARGQFDPQLYSSNAQKTFD
                     GTDYYNYNRPELNIPTWFGVNISTGLEYLSGSRTDPQETSGKTSYFGVTVPLAKNLLM
                     DKRRAALQTAKIYKDASQVEKNILLNNILQESIAAYWNWTLQYQVLQVLNDAIDVNRK
                     RVAFIKTSFRLGDRPGIDTIEAMAQLQNMELLQSQAALEFTNAGLGLSVYLWTPEQQP
                     YTLPAEIIPAAQPGLSELPVPVLDSLLNAARLNHPELRLYDYKLDALAVDKKLKFQEL
                     LPTVNFTYNQLGKGYDVLKTATGPLFENNFRYGFSVGIPLRFSAGRGEYKKARLKITE
                     AETEQRYKQLQVETKVRSYYNELVTLRQQLLLQENNYKNYKLLQQGEETRFKNGESSL
                     FLVNSRENKTLEALQKLQELRNKYVKTFNSLQWAAGQLM"
     gene            complement(1973..3289)
                     /locus_tag="PLU11_12250"
     CDS             complement(1973..3289)
                     /locus_tag="PLU11_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPN59945.1"
                     /translation="MTNLEYPQWSSFKKVYRYNKRSRVRYWFYGLMTALAIILFLPWT
                     QNIRAKGTVTTLRQEQRPQQLNTIIPGRVIKWYVKEGDYVKAGDTILQLAEIKDDYLD
                     PQLLSRTKEQLSAKSLTVDYYKNKVGTSGTQINALGEALQLKMDQLRNKLQQLQLKVQ
                     SDSMESIAATNDFKIATLQFSRQKAMYDSGLVSLTQLEQRNQSYQNSLAKKVSAENKL
                     QNARQDLTITRIEMNATQQDYMEKIAKAQGDQFQSLSQIATGQGDIAKLENQYSNYSI
                     RNGLYFITAPQSGQITKARKAGIGEIVKDSEMIVEIVPDQVQYAVELFVRPLDLPLVS
                     NGQKVRFMFDGFPAIVFSGWPNTSYGTFGGKVVAVESSVSYNGKFRILVTEDPADKPW
                     PRELKMGTGAQGMALLKDVPIWYELWRNINGFPPDYYKAGAEPEKK"
     gene            complement(3282..>4155)
                     /locus_tag="PLU11_12255"
     CDS             complement(3282..>4155)
                     /locus_tag="PLU11_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN59946.1"
                     /translation="QQLNIGQFIAAEIVIISVIASVEKLIGNLDSVYDVLTSVDKIAK
                     ITDKPVETSGTYLLPEKNEGLSIEAGNVCFAYNDEKYVLQNISFRINAGETVCITGKE
                     GSGKSTLLKIVSGSYQGFEGSLLLNELPVGNYELKSLRQQTGILLSQQDIFHGSLLDN
                     ITMGQPGIDVQQIIRLFKETGLADFLSSLPEGFDTWLDPTGKRLSRNVVNKILLVRAL
                     VGQPRLLLLEEPWTHIEEPYRSQIIRLLLKDYPNTTILVVSNDPEFTRLCDKVIDVKN
                     EGCIIKQNKTTDHD"
BASE COUNT          947 a          975 c          954 g         1279 t
ORIGIN      
        1 actggtattg tttctctatt ctttttcatc caggggcatg gctattacag cctggtggta
       61 cctgctgaca ggacatatct ttatactggc agctgtctta ttcaatgcac cgcatattta
      121 tttcagtgaa ttgtttttct acgccagcgg ggtgctgatc gcctttttac tgggctatta
      181 ctgcctgctt aagatagaac gtatcgatca ggatatttcc ttatcccgtt tccacggtta
      241 tgtatatgaa caggagaata ccggtttcct ctttctgttg gcggccatgg gcatgctggg
      301 tttcccgatc acggctgcgt ttataggcat tgatgttctg ttcacgtata ttcatagcga
      361 tcagtatgta ctgatcgcta tgctcggact atccctgttt tttgtagaac tggccgcctt
      421 ccggatcctg ctccgtatct tttttggccc gcataaaaaa ttatatcacc cggtggcatt
      481 ccggagtact taaccggcat tacatcagct gtccagccgc ccactgcaga ctgttgaacg
      541 tcttgacata cttgttgcgc aactcctgta atttctgcag cgcctcgagg gttttgtttt
      601 cccggctatt gaccaggaag agcgaactct ctccgttttt aaaccgggtt tcttcaccct
      661 gctgtagtag cttgtaattt ttataattgt tttcctgcag gagcagctgc tgacggaggg
      721 taaccagttc gttatagtaa gagcggactt ttgtttctac ctgcagttgc ttgtaacgtt
      781 gttctgtttc agcttctgta attttcaatc gcgctttttt atattcaccc cggccggccg
      841 agaagcggag gggaataccc actgagaatc cgtacctgaa attattttca aaaagcggac
      901 ctgttgctgt tttcaataca tcatatcctt tccccagctg gttataggta aagttgacgg
      961 tgggcagtaa ctcctgaaac tttaattttt tgtcgacagc cagtgcatcc agcttatagt
     1021 catatagacg cagttccgga tgatttagcc gcgcagcatt cagcaggctg tccagtacag
     1081 gaaccggcag ctcggataaa ccaggctgtg cagccggtat aatttcggcg ggtagggtat
     1141 aaggctgctg ttccggagtc cacaaataaa cagataatcc aagtccggca ttcgtaaact
     1201 caagtgcggc ctgcgattgc agtaattcca tattctgtaa ctgggccatg gcttcaatgg
     1261 tatcgatacc cggacgatca cccagccgga aactcgtctt aataaacgcc actcttttcc
     1321 ggttcacgtc tatcgcatca tttaatacct gaagcacctg gtattgaagc gtccagttcc
     1381 agtatgcagc aatagattcc tgtaatatat tattcaataa aatatttttt tccacctgcg
     1441 aagcatcctt ataaatcttc gcggtttgca aggcagccct gcgtttatcc atcagcaggt
     1501 ttttagccaa cgggaccgta accccgaaat aactggtctt acccgaagtt tcctgcgggt
     1561 cggtccggct acccgataaa tattccaggc cggtactgat attcacacca aaccaggtag
     1621 ggatattcaa ttcgggccgg ttgtagttgt aatagtcagt tccgtcaaac gtcttttgtg
     1681 cattggaact gtacagctgc ggatcaaatt gcccgcgggc aatggtaata tccgctttgg
     1741 ccatatcaat atttatatcg gcaatccggg ctacaggatg gtatcgttta acgatggaca
     1801 ttacctggct ggctgacagg gtttggagct gcagactgtc ctgtgccatc gaaagtttgg
     1861 caagaaacag caggatcagc atcagtcctg ttataaggat tgtttttttt cgttgctgat
     1921 tccgttgccg cagccagttg atgattttat attttgaatg cgtttgcatg gtttattttt
     1981 tttcaggttc agcaccggct ttataataat ccggcgggaa gccattgata ttccgccaca
     2041 gttcatacca gatgggtaca tctttcaaca gggccatgcc ctgggcgccg gtacccattt
     2101 tcaattcacg gggccagggt ttatctgccg ggtcttcggt aacaagtatg cggaattttc
     2161 cattatagct tactgaactt tccacggcca ccactttacc accaaatgtg ccgtaggagg
     2221 tattgggcca cccgctgaag acaatggcgg gaaacccgtc gaacataaac cgtactttct
     2281 gaccattgga aaccaggggg aggtcaagcg gacgtacaaa taactccacc gcgtattgta
     2341 cctggtcagg tacgatttcc acgatcattt cactgtcttt cacgatttct ccaattccgg
     2401 ctttgcgggc tttggtgatc tgtccgctct gcggtgccgt gataaaatac aaaccattac
     2461 gaatggaata attactgtac tgattttcca gtttggcaat atctccttgt ccggtagcaa
     2521 tttgtgaaag ggattgaaac tgatccccct gcgctttggc aattttctcc atatagtcct
     2581 gctgggtggc attcatttca atccttgtaa tcgtaagatc ctggcgggca ttctgtaatt
     2641 tattttccgc acttactttt ttggccaggc tgttctgata ggattgattt cgctgctcca
     2701 gttgggtaag cgacacaagg ccgctgtcgt acatcgcttt ctgccggctg aattgcagag
     2761 tggcgatttt gaaatcattc gtagcggcaa tggattccat actgtcactc tgtactttta
     2821 attgtaattg ctggagtttg ttgcgcaact gatccatttt cagttgcagg gcttctccca
     2881 gtgcattgat ctgggtgccg gacgtaccta ctttgttttt ataataatcc accgtaaggg
     2941 atttggccga taattgttct ttggtcctgc tcagcaattg cggatcaaga taatcgtcct
     3001 tgatctcggc cagttggaga atggtatccc cggcctttac ataatcccct tctttcacat
     3061 accatttgat gacccgtccc gggataatgg tattcagttg ctggggccgt tgttcctgcc
     3121 gtaaagtggt cacggttcct ttagcccgga tattctgtgt ccagggaaga aagagaatga
     3181 tggccagggc tgtcatcaat ccgtaaaacc agtagcgcac acggcttctt ttattatagc
     3241 ggtatacttt tttgaaggag gaccactgcg ggtattcaag attagtcatg gtcagttgtt
     3301 ttattctgtt tgataataca gccttcattt tttacatcaa ttactttgtc acaaagtcgg
     3361 gtgaattcag gatcattgct gacgacgagg atcgtggtgt tggggtaatc tttcagcagt
     3421 aaccggatga tctggctgcg gtagggttct tcaatatgtg tccagggttc ttcaagcagc
     3481 agcagccgcg gctgccccac gagcgccctg accagcagga ttttattcac tacatttctg
     3541 gacaatcgtt taccggttgg atccagccag gtatcgaatc cttccggcaa gctgctgagg
     3601 aaatcagcaa gtcctgtttc tttgaacagc ctgatgattt gctgcacatc aataccgggt
     3661 tggcccatgg taatattgtc caacaggctg ccgtggaata tatcctgctg gctcagcagg
     3721 ataccggttt gttgacggag tgatttcaat tcatagttgc ctaccggtaa ttcgttcagc
     3781 agtaatgagc cctcaaatcc ctgataggaa ccgcttacga ttttgagcag ggttgatttt
     3841 ccgcttcctt cctttccggt tatacaaaca gtttcgccgg cattgatcct gaagcttata
     3901 ttttgtaata catacttttc gtcattgtat gcgaagcata cattgcccgc ttcaatcgaa
     3961 agtccttcgt ttttctcagg cagtaaatag gtgccggatg tctctactgg tttatccgta
     4021 atctttgcaa ttttatctac ggaagtcagc acatcataca cgctgtctag attgccgatc
     4081 agtttttcaa cggaagcgat cacagagatg atcacaatct cagcggcaat aaactgcccg
     4141 atattcaatt gctga
//
LOCUS       DAOPMW010000297         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25374_length_4149_cov_6.457987, whole genome shotgun sequence.
ACCESSION   DAOPMW010000297 DAOPMW010000000
VERSION     DAOPMW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25374_length_4149_cov_6.457987"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLU11_12260"
     CDS             <1..773
                     /locus_tag="PLU11_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPN59947.1"
                     /translation="DKIINIFIIVLIILNVIAVMLETVPSFHDEAWEKDFFHYFDVIS
                     VIIFTVEYVLRVWSSNHEEKYRGTLKGRLRYIFSGAALIDLLAILPFYIHVIIGLDLR
                     VLRMLRLLRFVRLFRLTAYMKSAHMITNVFKKRFNELLLSLVLVIFLIIIASCAIYFA
                     EHLHPENKYKFTSIPATVWWSVVTLTTTGYGDMYPMTYLGKAMAMLIMLTGVAFFALP
                     AGIITAGFLEEFRLTRIRSTHRCPSCGEHIELDDHKHE"
     gene            complement(992..2329)
                     /locus_tag="PLU11_12265"
     CDS             complement(992..2329)
                     /locus_tag="PLU11_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN59948.1"
                     /translation="MKKLFNLVMLMMAAVASFAQANVLPAPAQKGVMYVKNVTIHVGN
                     GTVIENGVIVVRDGKIEKVGKDIVVPADATNVVDGQGKQVYPGLILPSSSLGLVEISS
                     IRASNDAREIGDMNPNVRSIVAYNTDSKVINTLRSNGILAANIIPQGSFLAGSSSVVQ
                     LDAWNWQDASLKTDGGMHLYMPSLMARPSFGRRGGGGPGGPNPGAESDPVKEGLQQLE
                     NLKGFFREAKAYLAAPTHEEVNLKFESVKDLLAKKQKFYVHANTVKQMLVALDFVKEF
                     GFDLVLVGASESWQIAPLLKQHNVSVILQQMHSLPTTTDDDVDQPYKSAAALQKAGVL
                     FAISDDDSQTRGRNLAFNAGTAVAYGLDKEQAIAAITLNAAKLMGVADKTGSIEVGKD
                     ANFIISSGDILDMATSNVTDAFIQGRKVNLDDKQKQLNDRFEQKYELKQKKGF"
     gene            complement(2459..>4149)
                     /locus_tag="PLU11_12270"
     CDS             complement(2459..>4149)
                     /locus_tag="PLU11_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN59949.1"
                     /translation="VFGVESLDLAGVTCHQRRPGGPGGFGGRGGMTEQPLPASATRLG
                     GVSNGTEWNGTGVDSLGNQLTWTAVFVKAADMKSDSVKKKDLPVIGKVMYPFLPYGWT
                     EEQQPKQETILIKNATVWTSEKDGVLKNTDVLLKNGKISAIGKNLNEPTARVIDGTGK
                     HVSAGIIDEHSHIAAASINEGAQSVTSEVRIGDNLNPEDINIYRQLSGGVTSSHILHG
                     SANVIGGQSQLIKLRWGAGDADLKFKTADGFIKFALGENVKRSSSTQGNNRYPDTRMG
                     VEQVLIDAFTRAKDYKKEWETYTAAKEQLVKAKKPLTGLNAPRRDLELEALVEILDRK
                     RFITCHSYVQSEITGLIEVADKMGFKVNTFTHILEGYKVADKMLKHGANASTFSDWWA
                     YKMEVEDAIPYNAAIMHKVGLNVAINSDDAEMARRLNQEAAKTMKYGGLTAEEALALV
                     TINPAKMLHVDDKVGSIKVGKDADIVLWSDNPLSIYAKSLYTIVDGTVYFDRQKDELL
                     QQEVQAERARLVKKLNGEKRSGAAMMPAMPSYQVMHSCSDHGHSHGLLVVDAEE"
BASE COUNT         1003 a         1131 c          850 g         1165 t
ORIGIN      
        1 gggataagat catcaatatc ttcatcattg tactgatcat cctgaatgtg attgccgtga
       61 tgctggaaac agtccccagt ttccatgatg aggcctggga aaaggatttc ttccattatt
      121 tcgacgtcat ttctgtaatc attttcactg tagaatatgt tttgcgggtt tggagcagta
      181 atcatgaaga aaaataccgc gggacgctaa aaggacggct caggtacatt ttttcgggag
      241 cggccttaat tgaccttctg gcgattctcc ccttctacat acatgttatt atcggcctgg
      301 atcttcgggt attacgaatg cttcgtttac tccggtttgt acggttattc cgccttaccg
      361 cttatatgaa gagcgcccac atgatcacca atgtattcaa aaaaagattc aacgaattac
      421 tcctgagcct ggtgttggtc atctttctca tcattattgc atcctgtgct atctattttg
      481 cggaacattt acatccggaa aataaataca agttcaccag tatacccgct acggtctggt
      541 ggtctgttgt caccctgacc accaccggct acggcgatat gtaccccatg acctatttag
      601 gcaaagccat ggccatgctg atcatgctta ccggtgttgc tttctttgcc ctccctgccg
      661 gtatcatcac cgccggcttc ctcgaagaat tccgcctcac ccgcattcgc agtacgcatc
      721 gctgcccgag ttgcggagaa catattgagc tggatgatca taaacatgaa taaaagttgg
      781 aggttggaag ttcgaggttg gaggtttcgt gctaagggta gtctgtcaca gtttcgcaaa
      841 aactttttat tccaactttt ttactgcctc ccttccttaa aacgaccgtt ctgcttgaag
      901 aacctccaac ttccaacatc caacctccaa cctccaacaa aaacgggctg ctttcagaag
      961 aaaacagccc gttgaattac ttattctttt cttagaaccc tttcttctgc ttcaattcat
     1021 atttctgctc aaagcggtca ttgagttgct tctgcttatc atccaggttg actttacggc
     1081 cctggataaa ggcatcggtt acattactgg tggccatgtc caggatatct ccggaactga
     1141 taataaaatt cgcgtccttt cccacttcaa tactgccggt cttgtcggcc acacccatca
     1201 gcttggcggc attcagggtg atggcggcaa ttgcttgttc cttatccaat ccatacgcta
     1261 cagctgttcc agcattaaaa gccaggttac ggccacgggt ttgggagtca tcatcagaga
     1321 tggcaaacaa aacacctgct ttctgtaaag cggcggcgga tttatagggc tgatccacat
     1381 catcgtcggt agtggtaggc agactatgca tctgctgtaa aatgaccgaa acattatgtt
     1441 gcttcagcaa tggagcaatc tgccagcttt cgcttgcacc caccagtacc aggtcaaacc
     1501 cgaattcctt cacaaagtca agggcaacca gcatttgttt aaccgtattg gcatgaacat
     1561 aaaatttttg ctttttagcc agcaggtctt tcactgattc aaacttcagg ttgacctctt
     1621 catgtgtagg tgctgccagg taagccttag cctcacggaa aaaacctttc aggttctcca
     1681 gttgctgcaa tccttctttt accggatcag attcagctcc cggattggga ccgcccgggc
     1741 caccccctcc acgtctgcca aaagacggac gggccatcag ggaaggcata taaagatgca
     1801 tacccccatc tgttttcagg gaagcatcct gccagttcca cgcatccagc tgcaccaccg
     1861 atgaagatcc tgccaggaaa ctaccctgcg gaatgatatt cgctgccagg attccattgg
     1921 agcggagggt gttgatcact tttgaatcgg tattgtacgc tacaattgaa cgcacattcg
     1981 gattcatatc cccgatctca cgggcatcat tggaggcgcg gatagagctg atctccacca
     2041 gtcccagtga actgctcggt aaaataagcc cgggataaac ctgcttaccc tgaccgtcca
     2101 ccacattggt ggcatcggcc ggcaccacaa tatcctttcc tactttttca attttaccat
     2161 cccttaccac aatcaccccg ttttcaataa cggtcccatt gcccacatgt atggtcacat
     2221 tttttacata catgactcct ttttgtgccg gagcaggtaa tacatttgcc tgggcaaatg
     2281 aagccaccgc agccatcatc agcattacca ggttaaataa ttttttcatt cgtttttatt
     2341 ttaatgtttt taatgatcca acttaattca gtctctcccc tactaaccac tcaccactca
     2401 ccactcacca ctcaccattt atcctccgaa gctttagcga aggaggactc accactcact
     2461 attcctccgc atccaccacc agcaatccat gactatgtcc atgatcactg cagctatgca
     2521 taacctggta agaaggcata gccggcatca ttgccgctcc gcttctcttt tccccattca
     2581 gttttttcac cagtcgggct ctttcagcct gcacttcctg ttgtaacagt tcatctttct
     2641 gacggtcaaa atatacagta ccatccacaa tcgtgtacag tgatttggca taaatactca
     2701 gcgggttgtc agaccacaat acaatgtcag catccttacc cactttgata ctgcccactt
     2761 tatcatccac atgcagcatt ttagcggggt tgatggtaac aagggccagc gcttcttcag
     2821 cggtcagacc accatatttc atggtcttgg ccgcttcctg attcaggcga cgggccattt
     2881 cggcatcatc tgagttgatc gccacattca atccaacttt atgcatgatg gcggcattat
     2941 aaggaatggc atcttccact tccattttat acgcccacca gtcggagaac gttgaagcgt
     3001 tggcgccatg cttcagcatt ttatccgcca ctttataacc ctcaagtata tgcgtaaacg
     3061 tatttacttt gaaacccatt ttatcggcca cttcgatcag accggttatt tcactttgta
     3121 cataggagtg acaggtaatg aaccgcttcc ggtccagtat ctccaccaat gcttccagtt
     3181 caaggtcgcg gcggggcgca tttaatccgg tcagcggttt cttggcttta actaactgct
     3241 ctttggcagc cgtatacgtc tcccattctt tcttgtaatc tttagcccgc gtaaatgcgt
     3301 cgatcagcac ctgctccaca cccatccggg tatccggata acggttattt ccctgagtgg
     3361 aagaagaacg cttcacgttt tcccccagag caaacttgat aaacccatcg gcggttttaa
     3421 atttcaggtc cgcatcgccg gctccccaac ggagtttgat cagttgcgac tgaccgccaa
     3481 tcacattcgc cgaaccatgc aggatatggg aggaagtaac gcctccgcta agctgacggt
     3541 aaatattaat gtcttccgga ttcaggttat ctccgatacg cacttcggaa gtcactgatt
     3601 gggctccttc attaatggaa gccgctgcga tatgcgagtg ttcatcaata ataccggcag
     3661 aaacatgttt accagtgcca tcgatcaccc tggctgtagg ttcattgaga tttttcccaa
     3721 tagcggatat ctttccattt tttaacagta catcggtatt cttcaacacc ccgtcttttt
     3781 cactggtcca taccgttgca tttttaataa gaatagtttc ttgtttgggt tgctgctctt
     3841 ccgtccagcc ataaggcaga aagggataca tcactttccc tatcaccggc aaatcctttt
     3901 tcttcacact atcagatttc atatcagccg cttttacaaa tacagcggtc caggtgagtt
     3961 ggttgcccag tgaatccacc ccggtaccat tccactcggt accattgctc acgccgccaa
     4021 gacgggtggc actggcagga agtggctgtt ccgtcatgcc accacgaccg ccaaatccgc
     4081 cgggaccacc cggacgacgc tggtgacacg tgacgccggc gagatcaaga gattccacgc
     4141 cgaacacgg
//
LOCUS       DAOPMW010000298         4137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25477_length_4137_cov_5.159481, whole genome shotgun sequence.
ACCESSION   DAOPMW010000298 DAOPMW010000000
VERSION     DAOPMW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25477_length_4137_cov_5.159481"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLU11_12275"
     CDS             <1..411
                     /locus_tag="PLU11_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN59950.1"
                     /translation="DRIIMSHVLEHIRKEDMIDTLAHIKKHLLKDGGVFLLMVPNAQS
                     PTGSYWRYEDFTHNILFTSGSCIYVLRAAGFTDIEFIDPDGTKHMNPLKRVIIKSLLA
                     YHRFKENCWNKILQTSFHKGSPRIYTFELKVAAR"
     gene            428..1159
                     /locus_tag="PLU11_12280"
     CDS             428..1159
                     /locus_tag="PLU11_12280"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPN59951.1"
                     /translation="MKSILKKWLTGLAFPHLRSSYSQSGEDIIICDLFTRLGIQHPSY
                     LDIGANEPAALSNTYRLYKRGSSGVCIEPNPRLFKKFREKRKRDTVLNAGVAFDSRKE
                     ADYYLFEGNAHGFSTFSKDDADFWVHTGNDKVGRFKIESVIKTPLVSVTDLITKYFQP
                     HPNLISIDVEGLDLQILKLIDFNSCKTEVICVETLIYEENNKENKNQELINFLIESGY
                     FIYADTYINTIFCRQEAYKATSVKS"
     gene            complement(1154..1897)
                     /locus_tag="PLU11_12285"
     CDS             complement(1154..1897)
                     /locus_tag="PLU11_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPN59952.1"
                     /translation="MEKIRTGIVNYLNTRPLIYGLERQPVAGQIELTGDYPARIAEML
                     INGDIDLGLIPVAVIPQLPEYHIVGDYCIGTEGEIASVALFSEVPMAEVEKVYLDYQS
                     RTSVALLRFLMREYWGISPEIILATGEDYRQEIRGQTAGLVIGDRALEQRKISTFIYD
                     LGSEWRKITGLPFVFAAWVSTKPLPENFVHDFNQGNALGLEKIDEIVAANPYPLYDLK
                     KYYTLHLSYRLDEAKKKGMQRFLEVIKDL"
     gene            1954..3297
                     /gene="purB"
                     /locus_tag="PLU11_12290"
     CDS             1954..3297
                     /gene="purB"
                     /locus_tag="PLU11_12290"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPN59953.1"
                     /translation="MELTPLTAISAIDGRYRKQVQHLDDYFSEYGLMKYRVMVEIEYL
                     LFLEKKKFFKLTPKAIKHLAKLAEDFGPEDAQEIKQIEFTTNHDVKAVEYFLKNELEK
                     CGAAEAKEWVHFGLTSQDINNTAIPLSWKHAIEYEYLPSLLNLNMELKLLVDEWKDIP
                     MLAHTHGQPASPTKLGKEIMVFAERIQNQIEQFIGVPFTGKFGGATGNFNAHHIAFPK
                     TDWIKFGNEFLQKIGLSRQQFTTQIEHYDSLAAHFDAMKRINNILIDFCRDIWTYVSM
                     EYFRQRTIKGEVGSSAMPHKVNPIDFENAEGNLGLANAILEHLSAKLPVSRLQRDLTD
                     STVLRNIGVPFAHTLIAFRSIEKGLSKLVVNDAKIYEDLDNNWAVVAEAIQTVLRREK
                     YPNPYEALKELTRGNNKIDKKAIHGFIDGLKVKEALKKELRKITPHNYTGISPFK"
     gene            3322..>4137
                     /locus_tag="PLU11_12295"
     CDS             3322..>4137
                     /locus_tag="PLU11_12295"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN59954.1"
                     /translation="MKKTLFSFLLLSVAFTVQSQPACNPKGDFSFTRNPCSAFDITYA
                     TNAAGFTAIRWQFGDGNTSSGSSSATHTYASLGNYQITLIIDYPSCSDTIVKTISVTQ
                     QSDAQLIQTVDTLLCSGSTKQLRSSPGFVYCWSPAAFLDNPLSPNPVTSTTQNITYYL
                     NSLSQGTNLITNGDFNLGNTGFTSQYFYTANNTTEGQYYVGNNPSSWYFAHYGCGDHT
                     TGTGNMLLVNGSPQADVEVWKTTVTVSPNTNYTFSTWICSISVPNPAKLAFAIN"
BASE COUNT         1246 a          953 c          829 g         1109 t
ORIGIN      
        1 gatcgtatca ttatgagcca tgtgttggaa catatccgga aagaagatat gatagacacc
       61 cttgctcata taaaaaaaca cttgcttaaa gacggtggtg ttttccttct gatggtgcct
      121 aacgcacaat ctcccacagg atcctattgg cggtatgaag attttacaca taatatactt
      181 ttcacctctg gctcctgtat atatgttctc cgtgcagcag gtttcactga tatagaattc
      241 atcgaccctg atggaacaaa gcacatgaat cctttaaaaa gggttattat caaatcatta
      301 cttgcttatc accgatttaa agaaaactgc tggaataaaa ttctgcagac atcatttcat
      361 aaaggaagcc cacgtatata tacttttgaa ctaaaagtcg cagcccgata aaaaaaccag
      421 cactctgatg aaatctattc tgaaaaaatg gctgactggg ctagccttcc cgcatttaag
      481 atcaagttat tcccaaagcg gggaagatat tattatttgt gatttattta caagacttgg
      541 cattcaacac ccttcctacc tggatattgg cgccaatgaa cctgcagctt taagtaatac
      601 ataccggctt tataagagag gcagcagcgg ggtttgtatt gaacccaatc ctaggttatt
      661 taaaaaattc cgggaaaaaa gaaaaaggga tacagttctg aacgccggag tagcatttga
      721 cagccggaaa gaagccgatt attacctgtt tgaaggaaac gcacacgggt ttagtacttt
      781 ttctaaagat gatgctgact tctgggttca taccggtaat gacaaagtag gtcgatttaa
      841 gattgaaagc gttataaaaa cacctttagt ttctgttaca gacctgatta caaagtattt
      901 tcagccacat ccaaatctaa tatccattga tgtagaaggt cttgatcttc aaatacttaa
      961 attaatcgat ttcaattcct gcaaaactga ggtaatatgt gtggaaaccc tgatttatga
     1021 agaaaataat aaggagaata aaaaccagga actcatcaat tttttaatag aatctggcta
     1081 ttttatctac gctgacactt atatcaatac cattttttgc agacaagaag catacaaagc
     1141 gacatctgta aaatcataaa tcctttatca cttccagaaa ccgctgcatc ccttttttct
     1201 tggcctcgtc cagccggtaa ctcaggtgaa gtgtataata tttcttcagg tcatatagtg
     1261 gatatggatt ggcggctacg atctcgtcaa tcttttccag cccgagcgca tttccctggt
     1321 taaaatcatg tacaaaattt tcaggcaggg gctttgtact gacccaggca gcaaaaacaa
     1381 aaggaagtcc ggtgatcttc cgccattcag acccaaggtc atagataaaa gtggagattt
     1441 tacgttgttc cagggcccgg tcaccaatca ccaatccggc cgtttgcccc ctgatctcct
     1501 gccggtaatc ttcgccggtt gccagtataa tttccgggct gattccccag tattctctca
     1561 tcaggaaacg aagcagggcc acagatgtac ggctctggta atcgagatag accttttcca
     1621 cttccgccat cggtacttca ctgaacaaag ccacagaagc aatttcgcct tcagtaccga
     1681 tacaataatc cccgacaata tgatattcgg gtaattgggg gatgaccgcc accggaatca
     1741 gaccgaggtc aatatcacca ttaatcagca tctccgcaat cctggccggg taatcgccgg
     1801 tcagttcgat ctgtccggcc accggttgcc tttccagtcc gtaaataagg ggcctggtat
     1861 tgaggtaatt aacaatgcct gtgcgaatct tctccaattg ggttattttt gcgctgcaaa
     1921 ggtaaaggat atccttatct ataaacaact cacatggaac tgactcccct caccgccatt
     1981 tcagccatcg acggacgcta tcgcaaacag gtgcaacacc tggatgacta tttctctgaa
     2041 tacggactga tgaaataccg ggtgatggtg gagatcgagt acctgctttt cctggaaaaa
     2101 aagaaattct tcaaactcac tcccaaagcc attaaacacc tggctaaact ggctgaggat
     2161 ttcggacccg aagacgccca ggaaatcaaa caaattgagt ttaccaccaa ccatgatgta
     2221 aaggcggtag agtatttcct gaaaaatgaa ctggaaaaat gcggggcagc agaagccaaa
     2281 gagtgggtgc atttcggcct gacctctcag gatatcaata atacggcgat ccctctttcc
     2341 tggaaacatg ccatagaata cgaatacctg ccctccctgc tcaatctgaa tatggaactg
     2401 aagttgctcg tggatgaatg gaaggatatt ccaatgttgg cccatactca tggacagccg
     2461 gccagtccga ccaaattggg caaagagatc atggtatttg ccgagcggat ccagaaccag
     2521 atcgaacagt ttatcggcgt ccccttcacc ggaaaattcg gtggcgccac gggtaatttc
     2581 aacgcccatc atatcgcatt tccgaaaacg gactggatca aattcggcaa tgaattttta
     2641 caaaaaatcg gattgagccg tcagcaattc acgacccaga tcgaacatta tgatagcctc
     2701 gctgcccatt ttgatgccat gaaacggatc aacaatatcc tgatcgattt ttgcagggat
     2761 atctggacct atgtatctat ggaatatttc cgtcagcgta ccataaaagg tgaagtgggt
     2821 tcatctgcca tgccgcataa agtcaacccg attgattttg aaaatgcaga aggaaatctc
     2881 ggactggcaa atgccattct ggaacacctt tcggccaaat taccagtgtc ccgtctgcaa
     2941 cgcgacctga ccgattccac tgtattacgg aatatcggcg taccctttgc gcataccctg
     3001 atcgccttcc gttcgattga gaaaggattg agcaagctcg ttgtgaatga cgcgaagatc
     3061 tacgaagacc tggacaataa ctgggcagtg gttgccgaag ccattcaaac cgtattgaga
     3121 agggaaaaat acccgaatcc ctatgaggcc ctgaaagaac taacccgggg gaataacaaa
     3181 atcgataaaa aagccataca tggttttatt gatggcctga aagtaaaaga agctttaaaa
     3241 aaggaattga gaaaaatcac cccgcataat tatacaggta taagcccttt taagtaaatt
     3301 catgcccaga ttcatctgta catgaaaaaa accttattct ctttcctgct gctatccgta
     3361 gcttttactg ttcagtccca accagcctgc aatccgaaag gggacttttc ctttacgcgg
     3421 aatccatgtt cagcattcga tattacttat gcgacaaatg ctgccggctt tacagccatc
     3481 cgttggcaat ttggagacgg gaatacttca tcagggagta gctccgccac acacacatat
     3541 gcaagtctgg gtaattacca gatcactttg attattgatt acccttcctg ttctgataca
     3601 attgtaaaga caatcagtgt tacccaacaa tcagatgcac aactgattca gacggtcgac
     3661 actttacttt gtagcggttc aaccaaacaa ttgagatcga gccctggctt tgtctattgc
     3721 tggagtccag ctgcttttct ggacaatcct ttaagcccaa atccggttac cagcactaca
     3781 cagaatatca cttattactt aaactcttta tctcaaggaa caaatttgat tactaacgga
     3841 gattttaatc ttggcaatac cggttttaca tcccagtatt tttacactgc aaataataca
     3901 actgaaggcc aatattatgt tggaaataat cccagctcct ggtattttgc ccactatggt
     3961 tgtggcgatc acacaactgg caccggaaat atgttgttgg tgaacggatc acctcaagct
     4021 gatgttgaag tatggaaaac aaccgttact gtaagtccca acacaaatta cactttctct
     4081 acctggatat gttctatctc cgttccaaat ccagccaaac tcgctttcgc tataaat
//
LOCUS       DAOPMW010000299         4132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25519_length_4132_cov_5.605838, whole genome shotgun sequence.
ACCESSION   DAOPMW010000299 DAOPMW010000000
VERSION     DAOPMW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25519_length_4132_cov_5.605838"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..647
                     /locus_tag="PLU11_12300"
     CDS             6..647
                     /locus_tag="PLU11_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN59955.1"
                     /translation="MKVAIADDHKIFRKGVILSLRPYSNIKFVQEAENGEDLLNGLPA
                     SEPDVILMDLRMPGKDGIEATKAVSKQYPQIKVLILSMYEDERFVYHLMENGANGYLL
                     KSAEPQEIRRAIMEVHEKGYYLNNFVNRILLKKSHSRQKVVPSLNSEITLSDKEKDVL
                     RFICMEFTAAEIAQKMEISPRTVEAIKDRLMERFGSKNTAGLVFFAVKNNLIE"
     gene            complement(660..1316)
                     /locus_tag="PLU11_12305"
     CDS             complement(660..1316)
                     /locus_tag="PLU11_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPN59956.1"
                     /translation="MALNKEQIAKRIARELKDGYYVNLGIGIPTLVANYIPAGMDVVL
                     QSENGLLGMGPFPFEGEEDPDLINAGKQTITTVPGSVFFDSAMSFGMIRAGKVDLTVL
                     GAMEVSERGDIANWKIPGKMVKGMGGAMDLVASARNIIVAMMHTNPKGESKLLPACSL
                     PLTGVGCVKKIVSDLAVLDITPHGFQLLERAPGVSVEEIKAKTAGKLMVEGEIPEIVF
                     "
     gene            complement(1353..2051)
                     /locus_tag="PLU11_12310"
     CDS             complement(1353..2051)
                     /locus_tag="PLU11_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPN59957.1"
                     /translation="MNKVVSGAEEAIRDIADGNTLMLGGFGLCGIPENCINALVKKGV
                     SGLTCISNNAGVDDFGIGLMLKQKQVKKMIASYVGENAEFERQLLSGELEVELIPQGT
                     LATRCLAAGYGMPAVFTPAGVGTEVADGKESRVFNNKEYLMEMAFDADFAIVKAWKGD
                     TQGNLVYKETARNFNPLMAMAGRVTIAEVEELVPAGELDPDHIHTPGIYVHRIFQGRA
                     YEKRIEQRTVRTRE"
     gene            complement(2107..2553)
                     /locus_tag="PLU11_12315"
     CDS             complement(2107..2553)
                     /locus_tag="PLU11_12315"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPN59958.1"
                     /translation="MKKALLLLGFGILVFSCNNKSEKPEKLLSRDKMEDILWDLMRAD
                     LFINNYMLVKDTALDKKKQGIELYSQILKLHKVSQDQFRESFLYYRSQPDELKVLMDS
                     LSHHSDSSQLKQAAKVPKADSMAEQVIVPEKKDTQRRTGIPRSKAE"
     gene            complement(2553..3467)
                     /locus_tag="PLU11_12320"
     CDS             complement(2553..3467)
                     /locus_tag="PLU11_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPN59959.1"
                     /translation="MHKRFLIAFFLFFLAGSITAQEIQARLTVIASKVSTQIDRKIFN
                     TLQTGLTNFLNARKWTNDVFQPSEKIQCSFLITIEKDLGNNVFEGKMTIQAARPAYNT
                     TYDCPLLNFQDDNFIFKYQEFQPIEFNENRVQGNDPLVGNLTAVLAYYVNIILGFDYD
                     SYSLRGGDVYFQKAWNIVNNAPEGREITGWKSFESLRNRYWLAENMNNSRFALMHDVL
                     YSYYRSGIDVFFENEEAGRSGIINALNFLNTLNTENPNSMVMQLFFQGRANELVKIFS
                     KAGGEQKSRAKELLTRLDVTNANQYKTL"
     gene            complement(3460..>4132)
                     /gene="coaBC"
                     /locus_tag="PLU11_12325"
     CDS             complement(3460..>4132)
                     /gene="coaBC"
                     /locus_tag="PLU11_12325"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPN59960.1"
                     /translation="QVFSNGALYGKKVLVTAGPTYEPIDPVRFIGNHSSGKMGIALAR
                     EMQARGAEVTLVLGPVSGESILPGIRVISVVTAAQMYEACMNEFPVSSIAVMAAAVAD
                     YTPAQTAAEKIKKTGETLPLELKKTRDILKSLGEIKRTDQVLVGFALETNDEKKHALA
                     KLENKNADMIVLNSLNDAGAGFGKDTNKVTLFTRDGKEFPFGTKPKQAVAADITDTII
                     QLYYA"
BASE COUNT         1036 a         1098 c          890 g         1108 t
ORIGIN      
        1 gaaccatcaa ggtagcgata gctgacgatc ataagatctt ccggaaagga gtcatactct
       61 ccctgcgccc atattccaat attaaatttg tacaggaggc cgagaacggg gaagacctgc
      121 ttaacggact gcccgcttct gagccggatg tgatcctgat ggacctgcgt atgccgggaa
      181 aagacgggat cgaagccacc aaggcggtca gcaaacaata cccccagata aaagtactga
      241 tcctgagtat gtatgaagat gagcgctttg tttaccatct catggaaaac ggggccaatg
      301 gctacctcct gaaaagcgcc gaaccgcagg aaatacgccg ggccatcatg gaagtccatg
      361 aaaaagggta ttacctgaat aatttcgtca accgcatcct gctgaagaaa tcccattccc
      421 ggcagaaagt ggttccctcc ctcaacagtg agatcaccct cagtgataaa gaaaaagatg
      481 tgctccggtt tatttgtatg gagtttacgg ccgccgagat cgcccagaag atggagatca
      541 gtccccgtac ggtagaagcc atcaaggacc ggcttatgga gcgattcggg agcaagaata
      601 ccgccggcct ggtcttcttt gcggtaaaaa acaacctgat agaataagcg cagaatggcc
      661 taaaaaacga tttccggaat ttccccctcc accatcagtt tacccgcagt tttagctttt
      721 atttcttcca ccgatactcc cggtgcccgt tccagcaatt gaaatccatg cggcgtaata
      781 tccagcaccg ccaggtctga tacaattttt ttgacacacc ctaccccggt cagcggcagg
      841 ctgcaggccg gtaataattt ggactccccc tttggattgg tatgcatcat ggccacaatg
      901 atattccggg cactggccac caggtccatc gctcctccca taccttttac catcttcccc
      961 ggaattttcc aattggcgat atccccccgc tcactgactt ccatggcccc caatacggtc
     1021 aggtctactt tgcccgcccg gatcataccg aaactcattg ctgagtcaaa gaagaccgaa
     1081 ccgggtaccg tggtgatggt ttgcttgccc gcattgatca ggtccggatc ctcctcccct
     1141 tcaaacggga agggccccat ccccaataat ccgttttcgc tttgcaatac cacatccatt
     1201 ccagccggaa tatagttggc taccagggtc gggataccga tacccaggtt gacataatac
     1261 ccatccttca gttcacgggc gatccgtttt gcaatctgtt ctttatttaa tgccataccc
     1321 tttgtttgtt aaatgaaata agcccgttac tgttactccc ttgtccgcac cgtccgttgc
     1381 tcaatccgct tctcatatgc acggccctgg aaaatacggt gtacataaat accgggtgta
     1441 tggatatgat ccggatccag ttcaccggcc ggcaccagtt cctccacttc agcaatcgtg
     1501 acgcgaccag ccatcgccat caggggattg aaattgcggg ccgtttcctt ataaacgagg
     1561 ttgccctgtg tatcaccttt ccaggctttt acgatggcaa aatcagcgtc aaaggccatt
     1621 tccattaaat attccttgtt attaaatacc cggctttctt ttccatccgc gacttccgta
     1681 cctactcctg ccggcgtaaa gaccgccggc ataccatagc ctgctgccag gcatcgggtg
     1741 gccaatgtac cctggggtat cagttccact tccagttccc cgctcagcaa ttgccgctca
     1801 aactctgcat tctcccccac ataagaggcg atcattttct tcacctgctt ttgcttcagc
     1861 atcaatccga tcccaaaatc atccaccccg gcattattgg aaatacaggt gaggccactg
     1921 accccttttt ttaccagggc attaatacaa ttctccggta taccgcataa gccaaatcca
     1981 cccagcatca gggtgttccc atcagcaata tcccggatgg cttcctcagc cccgctgaca
     2041 actttattca tgtaatcgtg attaggtagt aaaaataagg acttgcaagg aaattcaacc
     2101 gaacgttcat tctgctttac tccggggtat cccggtccgg cgttgggtat ctttcttttc
     2161 agggactatc acctgttcag ccatactatc ggcttttggg actttggcgg cttgctttaa
     2221 ctgcgaacta tcactatgat gactcaggga atccataagc actttcagtt cgtccggctg
     2281 agaacggtag taaagaaaac tctccctgaa ctgatcctgg ctgaccttat gcaattttaa
     2341 aatctggctg tataattcaa tcccttgctt ctttttatcc agggcggtgt ctttgaccag
     2401 catatagtta tttataaaaa gatccgcccg catcaggtcc cagagaatat cctccatttt
     2461 gtcacgcgac aacagttttt ccggcttttc cgatttgttg ttacaggaaa aaacaaggat
     2521 accgaagccc agcagtagca gtgccttttt cattacaagg ttttatactg gtttgcattg
     2581 gttacatcca gccgggtgag gagttctttc gcacggcttt tctgttctcc accagcctta
     2641 ctgaatattt tcacgagttc attcgctctt ccctggaaaa aaagctgcat gaccatggag
     2701 tttggattct ctgtattcag ggtattcaga aaattgagcg cgttgataat accgctgcga
     2761 ccggcttcct cattttcaaa aaacacatca atacccgagc ggtaatagga atataacaca
     2821 tcatgcatca gggcaaagcg gctgttattc atattttcgg ccagccagta ccggttacgc
     2881 agactttcaa aggatttcca gccggtgatc tcacgaccct ccggtgcatt attgacaata
     2941 ttccacgctt tttgaaagta gacatccccg ccacgcagtg aatacgaatc ataatcaaat
     3001 cccaggataa tgttgacata ataagccagg acggcagtca ggttgccgac cagggggtca
     3061 ttcccctgta cccggttctc attgaattca atgggttgaa attcctggta tttgaagatg
     3121 aaattatcat cctgaaaatt cagtaaggga caatcatacg tggtattata agccgggcgg
     3181 gccgcctgta tggtcatctt tccctcaaag acattattgc ccaggtcctt ttcgatggtg
     3241 atcaggaaac tgcattgaat tttttcggag ggctgaaata catcattggt ccacttacgg
     3301 gcattcagaa aattggtcag tcccgtctgc agggtattga aaattttccg gtcgatctgc
     3361 gtgcttactt tactggcaat caccgtcagc cttgcctgaa tttcctgggc agtaatggaa
     3421 cccgccagga aaaaaaggaa aaaggcaatc agaaaacgct tatgcatagt acaattggat
     3481 tatagtgtca gtaatatcag cagccacggc ctgcttgggt ttggtgccga aaggaaattc
     3541 tttaccgtca cgggtaaaca aggttacttt attggtatct tttccaaaac cggcgcctgc
     3601 atcattcagt gaattcagta caatcatatc cgcgttctta ttttccagtt tagccagcgc
     3661 atgtttcttt tcatcatttg tttcgagggc aaagcccacc aatacctggt ctgttctttt
     3721 tatctccccg aggcttttca atatatccct tgttttcttc aactccaggg gaagagtttc
     3781 accggttttc ttgatcttct ccgctgctgt ctgcgccggt gtataatcgg ccactgccgc
     3841 tgccatcacc gcgatcgatg ataccggaaa ttcattcata caggcttcat acatctgggc
     3901 agctgtaacc acggaaataa cccggatacc cggcaaaatt gattctccgg atactggtcc
     3961 cagtaccagg gttacttcag cgccccgggc ctgcatttca cgggcaaggg caatgcccat
     4021 tttcccggag gaatgatttc cgataaaacg aaccggatca atgggttcgt atgtggggcc
     4081 tgcggtaacc agtacttttt taccatataa agctccatta gaaaaaacct gc
//
LOCUS       DAOPMW010000300         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25679_length_4114_cov_9.721114, whole genome shotgun sequence.
ACCESSION   DAOPMW010000300 DAOPMW010000000
VERSION     DAOPMW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25679_length_4114_cov_9.721114"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLU11_12330"
     CDS             complement(<1..674)
                     /locus_tag="PLU11_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HPN59961.1"
                     /translation="MTSGPANSPKKGTRGVILGAAFLMATSAIGPGFLTQTSLFTGEL
                     LTSFGFVILISVLLDIGAQLNIWRIITVSGKRAQDISNTMLPGLGFFLAALIAFGGLV
                     FNIGNIAGTGLGLNVLTGIDVKIGAVISCVIALGIFWSKQAGSLIDVFVKVLGLLMIG
                     LTLYIAFISHPPLGEALYRTVWPEKTDMMKIVTLVGGTVGGYISFAGAHRLLDAGIKG
                     KEQLGQV"
     gene            complement(655..1398)
                     /locus_tag="PLU11_12335"
     CDS             complement(655..1398)
                     /locus_tag="PLU11_12335"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPN59962.1"
                     /translation="MRDHIDINCDMGEGMGNEEALMPFISSANIACGYHAGDTNSILH
                     TLELCKQFNVAPGAHPSFLDRSNFGRREMELPVTELYELIIQQLLIMQEMADMAEVSL
                     RHVKPHGALYNMSAREPLVAQVIAKAVKNFNSKLFLVGLSGSCSIEQAIEIGLNTISE
                     VYADRTYQEDGTLTPRGTAEAMITDASVAAAQVLEMVKEGTVTTVSGVKIPIVAESIC
                     LHGDGPHVLEFATTIHRNLKEKGIDIRPS"
     gene            complement(1391..2371)
                     /locus_tag="PLU11_12340"
     CDS             complement(1391..2371)
                     /locus_tag="PLU11_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPN59963.1"
                     /translation="MSLRIIKAGIQDTVQDLGRYGYQQAGINPSGAMDRYAMQVANAL
                     VGNELHEAVLELHFPSSVILFTRPLMIAITGADFSSSINGEPVPLNQGIVVNKNDALQ
                     FHRPVSGARSYLAVQGGFQMNHWLESQSTNLKATAGGYEGRALRKGDELICRTEGEYS
                     KYLNGKNFRILPWTADHQSGDNSKEILILPGKEWDRLPAASRERFLGTPFLITKLADR
                     MGFHLGNEPLDILPGEELVSSAVTFGTIQLLPDGKLIILMADHQTTGGYPCVAHVITA
                     HHSKLAQKRSAEALHFKMTDLETAEQLLQKQQQHIKQLQNACLFKLQPYA"
     gene            complement(2352..3116)
                     /gene="pxpB"
                     /locus_tag="PLU11_12345"
     CDS             complement(2352..3116)
                     /gene="pxpB"
                     /locus_tag="PLU11_12345"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPN59964.1"
                     /translation="MMGYVRVTKKHIIFNNTPMTTPTLSYQLFPLGDSALTLDWGNRI
                     DEAINLEVMARYQQFRHQQFPGFIEAVPAYSSLTVYYDPVFIRKLLPAEGTAFNYVRE
                     RITEIMAVELTYKETNSRMVRIPVCYDASLSPDLEHILEENKITVEELISLHTGRIYR
                     VYMMGFLPGFAYMGEVDEQLAMARKLRPKSTKAGSVGIAGRQTGIYPFTSPGGWQIIG
                     MTPIELFNRNRKEPTLLQAGDRVQFYSIDKYEFAHY"
     gene            3135..>4114
                     /locus_tag="PLU11_12350"
     CDS             3135..>4114
                     /locus_tag="PLU11_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPN59965.1"
                     /translation="MSRLNRRDSINLFSKLTFRRVWNGLKVLASYQVSKWTGKSVQWG
                     YPVSISFEPTTSCNLRCPECPSGLRAFTRPTGMLQRDFFRETIDQLHKELLYLVFYFQ
                     GEPYLNPSFLDMVLYAGSKKIYTATSTNAHYLTDDNAKRTIESGLDRLIISIDGTTQE
                     VYQQYRVGGQLSKVLEGARNIVKWKKELNSTKPFVVFQFLVVKPNEHQIEDVKALAKE
                     IGVDDVWFKTAQIYDYEKDPNQLIPTIDKYSRYRKNGDGYSFKGKLANHCWRLWHDPV
                     ITWDGLVAPCCFDKDAQHQLGDLKKKPFKEIWKNGEYSKFRQQILKGRKN"
BASE COUNT         1051 a         1043 c         1004 g         1016 t
ORIGIN      
        1 acctggccaa gctgttcctt tccctttatg cctgcatcaa gcaaacgatg tgcaccggca
       61 aaagaaatat aaccccctac cgttccccca accagggtca cgatcttcat catatcggtc
      121 ttctccggcc atacggtccg gtagagtgct tcgcccaggg gtggatggga aataaaagcg
      181 atgtacaggg tgagcccaat catcagtaaa cccagcactt ttacaaacac atcgatcaga
      241 cttcccgctt gctttgacca gaagataccc agggcaatca cgcaactgat gaccgcacca
      301 atttttacat caataccggt gagtacattt agtcccaatc ctgtgccggc aatattgccg
      361 atattaaata ccagtccgcc aaatgcaatc aacgccgcta aaaaaaagcc gagtcccggc
      421 agcatcgtat tggaaatatc ctgggcccgc ttcccactga ctgtaataat gcgccagata
      481 ttcaactgtg ccccgatatc cagcaatacc gatatcaata tcacaaaacc aaaactggtg
      541 agcagttcac cggtgaacaa actggtctgg gtaaggaaac ccggaccaat agccgaagtg
      601 gccatcagaa aagcagcacc gagaatgacg cccctggttc cttttttggg ggaattagct
      661 gggccggatg tcaatgcctt tctcttttaa gttgcggtga atagtggtgg cgaattccaa
      721 tacatgagga ccatcgccat gcaggcagat cgattcagcc acaatcggaa tttttacgcc
      781 ggatacggtg gtcacagtac cttctttaac catctccaat acctgggctg ccgctactga
      841 agcatctgtg atcatggctt ccgccgtacc cctgggtgta agcgtaccat cctcctggta
      901 ggttcggtcg gcatagactt cactgatcgt gttgagtccg atctcaatgg cctgttcaat
      961 ggaacaactg ccgctgagtc cgaccaggaa taatttactg ttgaaatttt ttaccgcttt
     1021 agcaatcact tgtgccacca ggggttcgcg ggccgacata ttatacaaag ccccatgcgg
     1081 ttttacatgc cggagagata cttcggccat atcggccatt tcctgcatga tgagtaattg
     1141 ctggatgatg agttcataga gttcagtgac gggcaattcc atttcccttc tgccaaaatt
     1201 tgaccggtcc aggaaggaag gatgtgcacc gggggccaca ttgaattgtt tacagagttc
     1261 cagcgtatgc aggatgctgt tggtgtcccc tgcatggtaa ccgcaggcaa tattggcgga
     1321 ggaaataaaa ggcatcaggg cttcttcatt gcccataccc tctcccatgt cgcagttgat
     1381 atctatatgg tcacgcatat ggttgcaatt taaataaaca agcattctgc agctgcttta
     1441 tatgctgttg ttgtttttgc agcagttgtt cagccgtctc cagatccgtc attttaaagt
     1501 gtaatgcctc tgccgatctt ttctgcgcca gctttgaatg atgggccgtg attacatggg
     1561 ctacgcatgg atagcctccg gtggtctggt gatcggccat caggatgatc agtttgccat
     1621 cgggcaataa ctggatcgtt ccgaatgtga cagcggatga aaccaattcc tccccgggca
     1681 ggatatccag gggttcattg cccaggtgaa aacccatccg gtcggccagt ttggtgatca
     1741 gaaaaggagt tcccaaaaaa cgttccctgg atgcagctgg caaccggtcc cattcctttc
     1801 cgggaagaat taaaatttcc ttactgttat cacccgactg gtgatcggct gtccagggca
     1861 gtatcctgaa atttttccca tttaaatatt tactatactc cccttcggtc cggcaaatca
     1921 gttcatcgcc tttccgcaaa gcccggcctt cataaccacc tgccgtggct ttgaggttgg
     1981 tactctgact ttcgagccag tggttcattt gaaagccacc ttgtacggca agataggaac
     2041 gggctccgct caccggacga tgaaactgca acgcatcatt tttattcacc acgataccct
     2101 ggttcagcgg taccggttcg ccattgatgc tggatgaaaa atcggctccc gtaatggcga
     2161 tcatcagtgg ccgggtaaag agtataacag atgaaggaaa atgaagttcg agcaccgctt
     2221 catgcagctc atttcccacc agcgcattgg ccacctgcat tgcatatcgg tccatggcgc
     2281 cggaaggatt gatccccgct tgctggtaac cgtagcgacc gaggtcctgc acggtatcct
     2341 gtatgcctgc tttaataatg cgcaaactca tatttatcga ttgaataaaa ctgcacccgg
     2401 tcacccgcct gcagcagtgt gggctctttg cggttacggt taaataattc gatgggggtc
     2461 atgccgatga tttgccagcc acccggagag gtaaatggat agatgccggt ctgccggccg
     2521 gcaatgccca cactgccggc tttcgtggac tttggcctga gcttgcgggc catggccagt
     2581 tgctcgtcca cttctcccat ataagcaaat ccgggtagaa aacccatcat gtacacccgg
     2641 taaatccggc cggtatgcag actgatcagt tcctccactg ttattttatt ttcttccagt
     2701 atatgttcaa gatccggcga caagcttgcg tcataacaaa ccggaatgcg taccatgcgg
     2761 gaattagttt ccttataagt tagttcaacc gccattattt cggttattcg ttcccgtacg
     2821 taattaaaag cggtaccctc cgcaggcagc agtttccgga taaacaccgg gtcataatac
     2881 accgtcaggg agctataagc cggaaccgct tcgataaaac cggggaactg ctggtgcctg
     2941 aactgctggt accgggccat gacttccagg ttgatcgctt catcaatacg attaccccag
     3001 tcgagcgtaa gggccgaatc gcccagggga aagagttgat atgataaggt gggggtggtc
     3061 atgggtgtgt tgttgaagat aatgtgtttt ttcgtcaccc gcacataccc catcataact
     3121 tacctttact cccgatgagc cgattaaacc gacgagacag tattaacctc ttttccaaat
     3181 taaccttccg gcgggtgtgg aacgggctga aagtgctggc cagttaccag gtcagcaagt
     3241 ggaccggaaa gtcggtgcag tggggttacc cggtttccat ttcctttgag cccacgacat
     3301 cctgtaatct gcgctgcccg gaatgcccaa gcggactgcg ggcctttacc cgtcccacag
     3361 gtatgctcca acgggatttt ttcagggaga ccatcgatca gctgcataaa gaattactct
     3421 atctcgtatt ttatttccag ggagagcctt acctcaatcc gtcttttctc gatatggtac
     3481 tgtatgcggg atccaaaaaa atttacactg ctacgtccac caatgcgcat tacctgaccg
     3541 atgacaatgc aaagcgcacg atagagagcg gactggaccg gctgattatt tcgatcgacg
     3601 gtaccacgca ggaagtatac cagcagtacc gggttggcgg ccagctcagc aaagtgctgg
     3661 agggggcccg taatattgtg aaatggaaaa aagaactgaa cagtactaaa ccctttgtag
     3721 tgtttcagtt cttagtggtg aaacccaatg aacaccagat tgaagacgta aaagcactgg
     3781 cgaaagaaat cggggtggat gatgtatggt tcaagaccgc tcagatttat gattatgaaa
     3841 aagacccaaa ccagctgatc ccgactattg ataaatacag ccggtaccgg aaaaacgggg
     3901 acggctattc attcaagggg aaactggcca atcactgctg gcggctctgg catgatccgg
     3961 tgattacctg ggacggactg gtggcgccct gttgttttga caaggatgcg cagcaccagc
     4021 tgggcgattt aaagaaaaaa ccgttcaaag aaatctggaa gaacggagaa tacagtaaat
     4081 tcaggcaaca gatcctgaaa ggaagaaaga atat
//
LOCUS       DAOPMW010000301         4104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25777_length_4104_cov_6.168190, whole genome shotgun sequence.
ACCESSION   DAOPMW010000301 DAOPMW010000000
VERSION     DAOPMW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25777_length_4104_cov_6.168190"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLU11_12355"
     CDS             complement(<1..1322)
                     /locus_tag="PLU11_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN59966.1"
                     /translation="MNISALSLQRPVLAIVMNIAIVLFGLIGFKFLGVRDYPAIDPPN
                     ISVRTSYPGANADIIETQITEPLEKAINGIAGVKNITSTSSNGTSNINVEFELSVDLE
                     AAANDVRDKVSQAQRSLPNDLDGPPVVSKADASSDPILSMTVQSNTKNQLEMTEYANN
                     VLVERLQTIPGVSGIQIWGEKRYAMRVWIDPAKLIAYNVTAGDVQSALLRENVELPSG
                     KISGNATELTVRTFGRLNTEDEFENLIVKNVGGADIKLKDVAQAVLGPENEETVLKGN
                     GTPMIALAVIPQPGSNYVAISDEFYKRLDQIKKDVPADVKIDIALDQTKFIKKSILEV
                     QETLMIAFLLVVMIIYAFFRDWIIALRPLIDIPVSLIGAFFIMYLMGFTINVLSLLAI
                     VLATGLVVDDGIVVTENIYKRIEKGMNKYQAALEGSKEIYFAVIATSIT"
     gene            complement(1378..2445)
                     /locus_tag="PLU11_12360"
     CDS             complement(1378..2445)
                     /locus_tag="PLU11_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN59967.1"
                     /translation="MRIKKYSLIALLVITAAACGDSKPKIQPPGAGSGPAKTPPMRVD
                     VYVVKPESFGESIEVPGNILANEVTEIHPEVSGRIVYLNQSEGKFISKGTLMAKLYDG
                     DLQAQLKKLQVQLALAKKTEERQSQLLKIQGISQQDYDISLLQVNNLNADIGIIQTSI
                     AKTEVRAPFSGKLGLRNISTGAYVTPASVIAVVNQTDILKLDFSVPEKYTSQIKIGQL
                     VSFSFEGSDKTHSAKIIATESNVTENTRSLLARAQVIGKDITLLPGAFAKVKLEFAPD
                     PNALLIPTQAVLPQARGKKAVLVKNGMAAFTDITTGIRDSSRIQILDGLKTGDTVVVT
                     GLLSIRPDAPVQVGKIINNKK"
     gene            complement(2582..3703)
                     /locus_tag="PLU11_12365"
     CDS             complement(2582..3703)
                     /locus_tag="PLU11_12365"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPN59968.1"
                     /translation="MQNIGILGGGQLGRMLLQAAANYPVTVHILENDPDCPSAHLCHQ
                     FTLGDIRDFEAVYNFGKNLDAITIEIENVNVEALEKLESEGVKVYPSPAVLKTIKNKI
                     LQKDYYKKHQIPTAEYLITENLAALEKAGDFLPAVHKVGEGGYDGKGVQLIRTREDMS
                     KGFDAPAVLEKMVPIHKEIAQMVAINEKGETALYPPVEMLFDPDLNLLDYQLCPAELS
                     TQTLYRVEAIALTVVRNFKSAGIFAVEMFVDKNGDVLVNETAPRVHNSGHHTIEAHYS
                     SQFDMLWRVMLGYPLGNTDPILPSIMVNIIGAEGFSGDVRYEGLEEVLKMDNAFVHLY
                     GKKQTKPGRKMGHVTILSKEKQDLLHKSNRVKRTLLPKT"
BASE COUNT         1005 a          991 c          932 g         1176 t
ORIGIN      
        1 gtgatggaag tggcgattac ggcaaagtaa atttcctttg aaccttccag ggcagcctgg
       61 tatttattca tgcccttttc gatccgttta taaatatttt cagtgaccac gattccatca
      121 tccaccacca gtccggtggc aagtacaatg gccagcaaag acagtacatt aatggtaaag
      181 cccatcaggt acatgataaa gaaggcaccg atcagcgaaa ccggaatatc gatcagggga
      241 cgcagggcaa tgatccagtc acggaagaac gcgtaaatga tcatcaccac cagcaggaaa
      301 gcgatcatca gggtttcctg cacttcaaga attgattttt tgataaattt tgtctggtcc
      361 agtgcgatat caattttaac atccgcaggt acatcttttt tgatctgatc caaacgctta
      421 taaaactcat ccgaaatagc tacataattg gaacccggct ggggaattac cgccaatgcg
      481 atcatcgggg tgccgtttcc tttgagtacg gtttcttcat tttccgggcc aagtacagcc
      541 tgtgccacgt ctttcagttt gatgtcggcg ccacctacat tttttacgat caggttttca
      601 aactcatctt cggtattcag tcgcccgaaa gtccggacag tgagttctgt ggcatttcct
      661 gatattttcc cggaaggtaa ttccacattt tccctgagca gggcggattg cacatcacca
      721 gcagtgacat tataggcaat taacttggcc ggatcgatcc ataccctcat tgcgtatctt
      781 ttttctcccc agatctgtat accgctcaca ccgggtatgg tctgcaatct ttccaccagt
      841 acattattcg catattcggt catttcgagc tggttcttcg tattgctttg caccgtcatg
      901 gaaagaatgg ggtcggagct ggcatctgcc ttggaaacta cgggtggtcc gtcaaggtca
      961 ttggggagcg accgttgtgc ctgggatact ttatcccgca catcgttggc cgcggcttca
     1021 aggtccacac tcagttcaaa ttccacattg atattactgg tcccattact gctcgtggac
     1081 gtaatgtttt ttacaccggc gatcccattg atcgctttct ccaatggctc agtgatctgt
     1141 gtttcgatga tatcggcatt ggcgccagga taggaggtac gtacactgat attcggcgga
     1201 tcaatagccg gatagtcacg cacaccgaga aacttaaatc caatcaatcc gaataacaca
     1261 atcgcaatat tcatcacgat ggccagaacc ggacgttgta aacttaatgc ggatatattc
     1321 atagcaggat ctttgtaacg cgtcgattat tcagaaaaaa gcgaataacc gaagtgctta
     1381 ctttttatta tttatgatct ttccaacctg gaccggagca tccggccgta tactcagcag
     1441 accggtaacc actacggtgt ctcctgtttt taatccgtcc aatatctgaa tccgggaaga
     1501 atcacggata ccggtggtga tatcagtaaa agctgccatt ccgtttttaa ccaggactgc
     1561 ttttttgccc ctggcctgag gcaataccgc ctgggtaggg atcaataagg cattgggatc
     1621 cggggcaaat tccagtttca ctttcgcgaa agcacccgga agcagggtaa tgtccttgcc
     1681 aatcacctgg gcgcgggcca gcaggctgcg ggtgttttcg gtcacattgg attctgtggc
     1741 gatgatttta gcgctatgtg ttttatcaga accttcaaaa ctgaaactaa ccagttgacc
     1801 aattttgatc tgtgaagtat atttttcagg tactgaaaaa tcaagtttga gaatatctgt
     1861 ttgattaact acggcgatca cagatgcggg agtgacataa gcacctgtac tgatattacg
     1921 cagtccgagt tttccgctga acggggccct gacttctgtt ttggcaatac tggtctggat
     1981 aatgccgata tccgcattca ggttatttac ctgaaggaga ctgatatcgt agtcctgctg
     2041 gctgataccc tggattttca ggagctggga ttgccgttct tctgttttct tcgccaatgc
     2101 cagctggacc tgtaattttt tgagctgggc ctgcagatca ccgtcatata atttggccat
     2161 cagggtaccc ttactgataa atttgccttc ggactggttg agataaacaa tccggccgga
     2221 tacttcagga tgaatttcag ttacttcatt tgccaggata ttaccgggca cttctatcga
     2281 ttccccgaag gactctggct taaccacata tacatctacc cgcatagggg gtgtttttgc
     2341 cggtccagac ccggccccgg gcggttggat cttcggttta ctatcgccgc aggctgcggc
     2401 cgttatcact aacaaagcga tcaggctgta cttcttgatt ctcatcttgt ttggtgccag
     2461 ttggtaaatt aatcaggagg ctaaaggtaa cagataatgc tggctattgg acgcgaaaac
     2521 cacctgattc ttgcacagaa tccgcaggaa ttataaacgg cctgacaggt ggataaaagg
     2581 atcaggtttt tggcaggagg gtgcgtttta cacggtttga tttatgcagc aaatcctgtt
     2641 tttccttgct aaggatcgtc acatgaccca ttttccggcc gggtttggtc tgttttttcc
     2701 catataaatg gacaaaggcg ttatccattt tcagcacttc ctctaatcct tcataacgca
     2761 catccccgct aaatccttcc gccccgataa tattgaccat gatagagggc agaatagggt
     2821 cggtatttcc caggggatat ccaagcatga cccgccagag catgtcaaac tgagagctgt
     2881 aatgggcttc aatcgtatga tggccactgt tatgcacgcg gggagccgtc tcattaacca
     2941 gcacatctcc atttttgtct acaaacattt caacggcaaa gatgcctgcg gatttgaaat
     3001 tccggacaac ggtcagggca atggcttcca cccggtagag ggtctgtgtg ctgagttcgg
     3061 ccggacaaag ctggtaatcg agcaggttga ggtccgggtc aaacaacatt tccaccgggg
     3121 gataaagcgc tgtttcacct ttttcattaa tagccaccat ctgggcgatc tctttatgaa
     3181 tgggtaccat tttttccaac acagccggag catcaaaccc tttactcata tcctcccgtg
     3241 tccgaataag ctgaaccccc tttccgtcgt acccgccttc gcccacttta tgaaccgctg
     3301 gcaggaaatc tccggccttt tccagggccg ccaggttctc agtaatcagg tattctgccg
     3361 tcggaatctg atgcttttta taataatctt tctgaagaat tttgttcttg atggtcttta
     3421 aaacggctgg ggacgggtat actttcacac cctcagattc aagtttttct aatgcttcaa
     3481 cattcacatt ttcaatctct atggtgatgg catctaaatt tttaccgaag ttgtaaactg
     3541 cctcaaaatc acggatatcg cccagggtga actggtgaca aagatgggcc gaaggacagt
     3601 ccgggtcatt ttcgaggata tgtacggtga ccgggtaatt cgctgccgcc tgcaggagca
     3661 tacggcctaa ctggcctcct ccgagtattc caatattctg cataggacaa agataatagt
     3721 tgaagtcctg cacttgcagg cccggtcttt tcttttttat atagatttgc catgtgattc
     3781 ctgactatcc ccataaagca ttcaccgacg aacgcggcca tttcagcctg cttatgcaga
     3841 cattggccat ggatcccttt tcctgaacct gactggtcca tttcttgttt tttaacctgt
     3901 gaccggtacg accacttttt tattttttca tctgccgcca gacggtaaac tgaattcatt
     3961 ttatgagtac aataatcact agcgaaaaga atcttactcc cactaccgat ttcctcccgc
     4021 tggaaggaac tgattatgtg gagttttata tagggaatgc caaacaggcg gcccactatt
     4081 atatgagtgc ctttgggttt cagg
//
LOCUS       DAOPMW010000302         4088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25897_length_4088_cov_7.181999, whole genome shotgun sequence.
ACCESSION   DAOPMW010000302 DAOPMW010000000
VERSION     DAOPMW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25897_length_4088_cov_7.181999"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLU11_12370"
     CDS             <1..263
                     /locus_tag="PLU11_12370"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPN59969.1"
                     /translation="FAAEMGRQPWVVYGLLRTSDALSASVKAEQVLISLILFTLVYAT
                     LFVLFLYLLNKKVKHGPEEHEHTSDAENSGYSTRENPLMKTK"
     gene            289..1317
                     /gene="cydB"
                     /locus_tag="PLU11_12375"
     CDS             289..1317
                     /gene="cydB"
                     /locus_tag="PLU11_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPN59970.1"
                     /translation="MLFGLDYSTWWFLVLGAVISGYAILDGFDLGAGALHLFLKKENS
                     RRIALNAIGPVWDGNEVWLVIAGGALFAGFPVAYAAVFSAFYLPAMIFLIGLIWRAVA
                     IEFRSKEPGKTWRLTWDIIYSAACIIVTLSLGLMLGNIAMGLPLNEQKEFTGSTWSFF
                     NPFSIMVAFTTLALFMMHGAIYLAMKTENRLYTKLTLLARNFTVFFLLSFAITTVYTL
                     LYIPHLSDKLKSSPTYFIFPVIMFLAIANIPRQLNKGNYRFAFISSAVTIAALLITVA
                     IEVYPNLLYASNNPANSITITNASSSLKTMKTLLLIAAIGTPLVGIYTSFVFWTFKGK
                     VKLDEMSY"
     gene            1467..2729
                     /locus_tag="PLU11_12380"
     CDS             1467..2729
                     /locus_tag="PLU11_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPN59971.1"
                     /translation="MPIRAIRGCHLPQANSSVAAIRYLYCFMNVFTDKSTIIITCPKR
                     LAPYLEQEVREMGYNTDETFITGVKLTGTVTECIRLNLNLRCASQVLYKIKQFTAVNA
                     DDIYKHLLDYPWENILPDDGYFSITSNVNNPTINNSMFANLRVKDAIVDRLREKRGVR
                     PSTGAELSGAVINLFWKNENAEVFIDTTGDSLGRHGYRKIPGQAPMLEALASATIYAT
                     TWDRQSPFVNPMCGSGTLAIEAAMIATNRRPGLFRTNYAFMHLNGYDETVYFKEDALL
                     EEQIKEVPGLRIIATDYSSRAIENSRKNAVAAGVADLIEFGVCDFADTEVPQGVPGVF
                     FVNPEYGERLGDIRELEETYARIGDFMKQKCGGYFGYVFTGNLELAKKIGLKAKRRIE
                     FYTSKIDCRLFEYELYSGSRSAPKKETN"
     gene            2747..3016
                     /locus_tag="PLU11_12385"
     CDS             2747..3016
                     /locus_tag="PLU11_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPN59972.1"
                     /translation="MEIAALQQIVRDRYFENDNARGIYHTALWFHEEVGELSSAIARG
                     DKENAKEEFGDVLMWMLTLANLMNVDMEEVVANYLDNPRKKLPGK"
     gene            3027..3716
                     /locus_tag="PLU11_12390"
     CDS             3027..3716
                     /locus_tag="PLU11_12390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN59973.1"
                     /translation="MEMILDNPIWHALSTHHRNFNEGDDRLKYFYPEISPFVAMADWD
                     EEDKEYLVEKLPADRNFFYITAQSLDLPEHCKRGFTIKLFQLVCRKHLPFHPPGIQIR
                     SLGKADIPQMIELTALTKPGPFNQRTIEFGNYIGIFEEERLVAMAGERLKFPGYTEVS
                     AICTHPDYTGRRYSAALLSQATAGIVSEGQTPFLHVRNDNTRAIDLYEQAGYEFRCEM
                     DFAIFKKPSFG"
     gene            complement(3713..>4088)
                     /locus_tag="PLU11_12395"
     CDS             complement(3713..>4088)
                     /locus_tag="PLU11_12395"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN59974.1"
                     /translation="LTTITKTNHMPLQSAVPILAFLDATESAAFYKKIGFDCNDNWKE
                     YLMCSRDKIEIHLWKCDDPAIPKNTGCYIRVDEIDALYKEFNNLGIIHPNGPLENKPW
                     GMRQFSILDNSGNIIHFGQYLD"
BASE COUNT         1098 a          932 c          925 g         1133 t
ORIGIN      
        1 ggtttgccgc ggaaatgggt cgtcagccct gggtggttta tggccttctt cgtacctccg
       61 atgccttatc cgcatcggta aaggcggaac aggtgttgat ctccctgata ctattcacgc
      121 tggtttatgc cacattattt gtcttattcc tttacctgtt gaataaaaaa gtgaagcatg
      181 gccctgaaga gcatgagcat acaagtgatg cggaaaattc cggctacagt acccgtgaaa
      241 acccattgat gaaaacaaaa tagtttttaa aaccagtaaa caaagtatat gttgttcgga
      301 ctcgactatt caacctggtg gtttcttgtt cttggtgcag ttatctcggg ttatgctatc
      361 ctggacgggt ttgatcttgg tgccggggca ctacatctgt ttcttaaaaa agaaaacagt
      421 cgccggatag ccctgaacgc aatcggtccg gtatgggatg ggaatgaagt ctggctggta
      481 attgccggcg gtgcactttt tgccggtttt ccggtcgcct acgcagccgt attttccgct
      541 ttttatctgc cggcaatgat cttcctgatc ggactcatct ggcgtgcagt ggccattgaa
      601 tttcgcagta aggagcctgg aaaaacctgg cggcttacct gggatatcat ttattccgca
      661 gcctgtatca tcgttaccct gtcacttggt ctgatgttgg gtaatatagc catggggctg
      721 cccctgaatg aacaaaagga atttaccgga agtacctggt ccttttttaa tcctttttct
      781 attatggtcg ctttcacaac actcgcattg tttatgatgc atggggctat ttacctggcg
      841 atgaaaacgg agaaccggct ctatactaaa ttaacattac tggccaggaa tttcactgtc
      901 ttcttccttt tatcctttgc tattaccacg gtttatacat tactttatat tccgcatctc
      961 agtgataaac taaaaagcag tcccacctat tttatatttc cggtaatcat gtttctggct
     1021 atcgccaata ttccccggca gttgaataaa ggaaattacc gctttgcctt tatcagttcc
     1081 gctgtaacca tcgctgcctt gctgatcacg gtggctattg aagtttatcc caacctgttg
     1141 tatgcaagta acaatcctgc aaacagcatt acaataacga atgcctcttc ctctttaaaa
     1201 accatgaaaa cattactgct gattgccgca attggcaccc cgctggtggg catctatacc
     1261 agttttgttt tctggacatt caaaggaaag gttaaactgg atgaaatgag ctattaactg
     1321 cagcaacagc tccacatacc tgcctgtgtc catctatgtg atccgcgttc atccatggct
     1381 taccttttgc cgctggccaa tcctttggtg taccttttgt ggcctttagt ggccaccctt
     1441 ttttgccgca ggcaaaacta aaatccgtgc ctatccgtgc tatccgtggc tgccatttac
     1501 cacaggcaaa ttcatccgtg gcagcaatac gttatcttta ctgcttcatg aatgtattta
     1561 ccgataaatc cacaatcatt ataacctgtc ccaaacggct tgccccatac ctggagcagg
     1621 aagtaagaga aatgggctat aacactgatg aaacttttat taccggagta aaactcaccg
     1681 gcaccgtgac cgagtgtatc cggctcaacc tcaatctccg ttgcgccagc caggtattgt
     1741 ataaaataaa acagtttacc gctgttaatg cagatgatat ttataagcac ttacttgatt
     1801 atccctggga aaacatatta ccggatgatg gctacttctc catcaccagt aatgtgaata
     1861 atcctacgat caacaacagt atgtttgcca acctgcgggt gaaagatgcg attgtagacc
     1921 ggttacggga gaaaagaggg gttcgtccca gtaccggagc tgaactcagc ggggccgtga
     1981 tcaatctgtt ctggaaaaat gaaaacgccg aggtatttat tgataccacc ggtgattcac
     2041 tgggacgaca tggttaccgg aaaattcccg gacaggcacc gatgctggaa gcactggcct
     2101 ctgccactat ttatgccacc acctgggacc ggcaatcgcc ctttgtgaat ccgatgtgcg
     2161 gttcgggtac actggccatt gaagcggcca tgatcgccac caaccggaga cctggattat
     2221 tccgcacgaa ctatgccttt atgcatctga atggatatga tgaaaccgtt tattttaagg
     2281 aagatgcatt attggaagaa cagatcaaag aagtcccggg actccggatt attgccaccg
     2341 actatagctc gcgggccatt gaaaattcaa ggaagaatgc ggtagctgcc ggcgtagccg
     2401 acctgatcga atttggggtc tgcgattttg ctgatacaga agtaccgcaa ggagtacccg
     2461 gtgtcttttt cgtgaatccc gaatatggcg aacggctggg tgatataagg gaactggaag
     2521 aaacctatgc ccggatcggc gattttatga aacaaaaatg tggcggctat tttggttatg
     2581 tgtttaccgg caatctcgaa ctggctaaaa aaatcggcct gaaagccaaa cgccggattg
     2641 aattttatac cagtaaaatt gactgccgcc tctttgaata tgaattatac agtggcagcc
     2701 gttcagcacc caagaaagaa accaactaat caataatatt cagtctatgg aaattgcagc
     2761 actgcaacag atcgtcaggg accggtattt tgaaaacgac aatgcccggg gtatttatca
     2821 taccgcttta tggtttcatg aagaagtagg cgaactctct tccgccattg cccggggtga
     2881 taaagaaaat gccaaagaag aatttgggga tgtcctgatg tggatgctca ccctggccaa
     2941 tctcatgaac gtagacatgg aagaagtggt ggccaattac ctggataatc caagaaaaaa
     3001 actaccgggg aaataacacg gctgccatgg aaatgatatt agataatccc atctggcatg
     3061 cattaagtac ccatcaccgg aattttaatg aaggggatga tcggcttaaa tatttctatc
     3121 cggaaatatc tccctttgtg gccatggctg actgggatga agaggacaaa gaatacctgg
     3181 tggaaaaatt acctgctgac cgtaattttt tttacatcac tgctcaatca ctggatctgc
     3241 ctgagcactg caaaagagga ttcactataa aactgttcca actggtttgc cggaaacatt
     3301 tgccgtttca tccgccgggt atccaaatcc ggtcattggg taaagccgat attccgcaaa
     3361 tgatcgagct gacggccctg accaaaccgg gtccatttaa ccaacgtact atagagtttg
     3421 gtaattatat tggcatattt gaagaggaac gcctggtagc catggccgga gaacgtttaa
     3481 aatttcccgg ttatactgaa gtgagtgcga tctgcactca ccctgattat acaggcagaa
     3541 gatacagtgc ggctttattg tcacaggcca cggccggcat tgtgagcgaa ggacagaccc
     3601 ctttcctgca tgtgcggaat gataatacaa gggccattga tctgtatgaa caagccggct
     3661 atgaattccg ttgtgaaatg gattttgcta tttttaaaaa accgtcgttc ggctaatcca
     3721 ggtactgccc gaagtgaatg atattcccgc tgttgtccaa tatactgaac tgccgcattc
     3781 cccagggctt gttttccagc ggaccattgg gatggataat acccaggtta ttaaattctt
     3841 tatagagcgc atcaatctca tccacgcgaa tataacagcc ggtattctta ggaatagccg
     3901 gatcatcaca tttccataaa tggatctcga ttttatcccg gctgcacatg agatactcct
     3961 tccagttatc attgcagtca aatccaattt ttttatagaa tgctgccgat tcggttgcat
     4021 ccaaaaaggc caggatcggt acagcagatt gcaagggcat atgattggtt tttgttatag
     4081 tagttaat
//
LOCUS       DAOPMW010000303         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_25979_length_4077_cov_9.144455, whole genome shotgun sequence.
ACCESSION   DAOPMW010000303 DAOPMW010000000
VERSION     DAOPMW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25979_length_4077_cov_9.144455"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1139)
                     /gene="thiL"
                     /locus_tag="PLU11_12400"
     CDS             complement(105..1139)
                     /gene="thiL"
                     /locus_tag="PLU11_12400"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPN59975.1"
                     /translation="MSTERTEISNLGEFGLIDHLTRNIEIQNASTVLGVGDDAAVIDH
                     FGKQTVLTTDLLVEGVHFDLIYTPLKHLGYKSVVVNLSDVYAMNAIPTQIILSIGISN
                     RFSLEALDEFYEGVYAACEKYGVDLVGGDTTSSQKGFIISLTAIGEVAPDNYVKRSTA
                     KKGDLLCVSGDLGAAYVGLLFLEREKKIFIESPGVQPDLEGETYVIGRLLKPEARKDI
                     VEFFAANQIKPTAMMDISDGLSSEILHICKDSNLGCVLYEEKIPVSEEMKKAAFKFEI
                     DPTACALSGGEDYELLFTVAQEDFEKLVLNEQISVVGYMTEPELGSHIITKGGSKHPV
                     TAQGWNHLQP"
     gene            complement(1136..1915)
                     /locus_tag="PLU11_12405"
     CDS             complement(1136..1915)
                     /locus_tag="PLU11_12405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPN59976.1"
                     /translation="MLKTALITAAKAGAAEILRFFNGEFKVSNKEGVNNLVTEADHAA
                     EKAILSVIKSHFPDHQVLAEETGEIVQDSVYKWIIDPIDGTVNFAHGIPLNCVSIGLE
                     HEGNIIMGAVYNPHLNEFFFAEKGKGATLNDQPIRVSKETAAVKACLVTGFPYTYINM
                     PNGPLEIFDRFIRKGVPVRRLGSAAIDLCWVAAGRFDGFYEHKLEPWDSAAGFLMVEE
                     AGGKVTDFTGAPYSVYQHRILATNGFIHEEMLAVINNEKEL"
     gene            2020..3972
                     /locus_tag="PLU11_12410"
     CDS             2020..3972
                     /locus_tag="PLU11_12410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN59977.1"
                     /translation="MPRLELSVIIVNYNVKHFLEQCLYSVRQALKKTSGEIIVIDNCS
                     SDNSLAYLQPDFPDVRFVANKENLGFARACNQGWKMSSGRFVLFLNPDTILPEDCFEA
                     CIRFFDEHPEAGALGSKMLDGSGTFLKESKRAFPSPLTSLYKLFGLSKLFPRSAHFSR
                     YHLGHLDENKNNEVDVLAGAYMMIRKEVLDQVGGFDERFFMYGEDVDLSYRIQKSGQK
                     NYYFAGSPIIHFKGESTRKASMNYVRMFYSAMSLFVRKHYGGRQAGLFNSLIHLAIWF
                     RAALSASGQFIRRIGLPLIDAVLILFSFWIIKEGWSEYVRPDIQYENRLLWMAIPGFT
                     LLYLVTAYYAGLYDKWYRWSELIRSTAVATIILLAAYSLLPEQFRFSRAIVLFGAVLA
                     FILISTLRYLLIKAGTISSEKNKEAHTTFVVAGSVSEYKEVIRLLEEAGFRQQVLGRV
                     AVETNDTTAIGHISQLPALAASLPFREVIFCQGTLSFTAIIEAIRGLPRGITARIHAA
                     NSHSVVGSDSKDSSGEAVSKENGYQLADPYQLRLKRGADVLVALISLATFPLLLFLVK
                     KPAGLLVNIFMVLLARKTWIGYTGVAEGLPVIRKGVLGCNGMPPHQSLQLSAESLYRA
                     DYWYAHDYTPAADIKLIIRFYRNLGG"
BASE COUNT         1013 a         1013 c          953 g         1098 t
ORIGIN      
        1 gggccgggga aaaggaggac ttgctcacgc cacaggcagt aagcaggatg gtacaacaaa
       61 acatcagtgt tctcctcata ggatctcagg ttccctgttt tattttatgg ctgcaggtga
      121 ttccagcctt gcgcggtgac gggatgtttg cttccgccct tggtaatgat atgcgaaccc
      181 agttcgggtt cagtcatata cccaaccacg ctgatctgct cattcaggac cagtttctca
      241 aaatcttcct gggccacggt aaagagcagt tcataatcct ctcccccact taacgcacaa
      301 gccgtgggat ctatttcaaa tttaaaggcc gcttttttca tttcctccga tacaggaatt
      361 ttttcttcat ataaaacaca gcccaggtta ctgtctttgc agatatgcag gatttctgag
      421 ctcaggccat cgctgatatc catcatggcc gttggtttga tctggttggc ggcaaaaaac
      481 tccacaatgt ctttccgggc ttcgggctta agcaggcggc cgattacata ggtttctcct
      541 tcgagatcag gctgcactcc cggactttca ataaatattt ttttctccct ttccaggaac
      601 aacaatccaa cgtaggccgc tccgagatca ccacttacac aaagcaggtc gcctttttta
      661 gcggtgcttc tttttacata attatccggc gccacttccc cgatcgccgt cagggaaata
      721 ataaatcctt tttgcgatga agtggtatca ccccccacca ggtccacccc gtatttctca
      781 caggcggcat ataccccttc ataaaactca tccagggctt cgagactgaa acggttgctg
      841 atgccgatac ttaagatgat ctgtgtggga atggcattca tcgcatatac atcactgagg
      901 ttgaccacca cgcttttata ccccaggtgt ttcaacgggg tatagatcag gtcaaaatgc
      961 acgccttcca cgagcaggtc agtggtcagt acggtttgtt tgccgaaatg atcgatcacc
     1021 gcagcatcat cgcccactcc caatacagta gaagcgtttt gtatttcgat attacgggtg
     1081 aggtggtcaa tcaggccaaa ttcacccagg ttgctgattt ctgttctttc tgtactcata
     1141 attctttttc attattaata acggccagca tttcctcatg gataaaacca ttggtggcca
     1201 gtatccggtg ctggtaaacg gaataagggg cgccggtaaa atcagtgact ttaccacccg
     1261 cttcttccac catcaggaaa cccgccgcac tatcccatgg ttccagttta tgttcataaa
     1321 atccatcaaa gcgacccgct gccacccagc aaaggtcaat ggcggccgag cccagccggc
     1381 gtaccggtac ccccttgcgg atgaaacggt caaatatttc cagcggccca ttgggcatat
     1441 tgatatacgt ataggggaaa cccgtcacca ggcaggcttt caccgccgcc gtttctttac
     1501 ttacccggat cggttgatca ttcagggtgg cacccttgcc tttctctgca aagaaaaatt
     1561 cattgaggtg cggattatac acggcaccca tgatgatgtt cccttcatgc tccaatccga
     1621 ttgacacaca gttcagcgga atgccatgag caaaattcac cgtcccgtcg atcgggtcaa
     1681 tgatccattt ataaacagaa tcctgtacga tctcaccggt ttcttccgcc agcacctggt
     1741 gatccggaaa atgggattta atcaccgaga ggatggcttt ttcagctgca tgatcagctt
     1801 ctgtaaccag gttgttgacc ccttctttat tactcacttt aaattccccg ttgaaaaacc
     1861 ggagaatctc tgctgcgccg gctttggcag ctgttatcag ggcagtttta agcatgggca
     1921 aagatagacc gcagaaggcg atgcgggaaa tgaatttata gcaatggtaa aggctatttt
     1981 gcactccagt aaaaaacttc gtacttcgtg tctataagta tgccccgctt ggagttgtcg
     2041 gttatcatag tgaattataa tgtaaaacac tttctcgaac agtgtcttta ttctgtccgg
     2101 caagccctta aaaaaacaag cggggaaatc attgtcatcg acaattgctc ctcagacaac
     2161 agcctggctt acctgcagcc ggatttcccg gatgtacgtt ttgtagccaa taaagaaaac
     2221 ctgggctttg cccgcgcctg taaccagggc tggaaaatga gctccggccg ttttgtcctg
     2281 tttctcaacc cggataccat ccttccggaa gattgtttcg aagcttgtat ccgttttttc
     2341 gatgagcatc cggaagcggg agccctgggc agtaaaatgc tggatggcag cggtaccttc
     2401 ctcaaagaat ccaaaagggc ctttccatcg cccctgacct ctctctataa gttgtttggt
     2461 ttatcaaaac tattcccacg atcggctcat ttctcccggt accacctggg tcacctggat
     2521 gaaaataaaa acaatgaagt ggatgtgctc gcaggggcct acatgatgat cagaaaggaa
     2581 gtactggacc aggtaggtgg ttttgatgaa cgtttcttta tgtatgggga ggatgtagac
     2641 ctgagttacc ggattcagaa aagcgggcag aagaattatt atttcgccgg aagtcccatt
     2701 atccacttta agggagagag tacccgcaag gccagcatga actatgtccg gatgttctat
     2761 tctgccatga gcctgtttgt gcgcaaacat tacggtggac ggcaagccgg attgttcaac
     2821 agtctcattc acctcgcgat ctggttcagg gctgcactca gcgcatccgg acagtttatc
     2881 cggcggatcg gtttgcccct gatcgatgcg gtactgattc tgttttcgtt ctggatcatc
     2941 aaagaaggct ggtcggaata tgtccgaccg gatattcaat atgaaaaccg cctgttgtgg
     3001 atggccattc ccgggttcac tttgttgtac ctggtcacgg cctattatgc aggactctac
     3061 gataaatggt accgctggag tgaattgatc cggtccaccg cggtggcaac gattatactc
     3121 ctggcggcct actcactttt acccgaacaa ttccgttttt caagggccat tgttttattc
     3181 ggtgcggtgc tggcttttat attaatcagt acgcttcgtt atttattgat aaaagccgga
     3241 acgatcagca gtgaaaagaa taaagaagca cataccactt ttgtggtggc aggttcggtc
     3301 agtgagtaca aagaagtcat ccggttattg gaagaagccg gattccgtca gcaggtattg
     3361 ggacgggtag ctgtggaaac caacgatacc actgccatcg gtcatatcag tcagctcccg
     3421 gcattggccg ccagtcttcc gttccgggaa gtgattttct gccaaggcac cctttccttc
     3481 accgcaatca ttgaagcaat ccgtggactg ccccgcggta ttacagcaag aatacatgcg
     3541 gcaaatagtc acagtgtagt gggcagtgat tcgaaagaca gttccggtga ggccgtatca
     3601 aaagaaaatg gttaccaact ggccgatccc tatcagttac gtctgaaaag aggagcagat
     3661 gtattggtgg cactcattag tctggccact ttccctctct tattattcct ggtcaagaaa
     3721 cccgccggat tgctggtgaa tatttttatg gtattactgg cccgtaaaac atggatcggg
     3781 tatacgggtg tggcggaagg gcttccggtt atccgaaaag gagtactagg ctgcaacggc
     3841 atgccgccgc atcaatcatt acaactgtcc gctgaaagtc tgtaccgggc tgattactgg
     3901 tatgcgcatg attatacgcc ggcggcagat atcaaactga ttatacggtt ttacagaaac
     3961 ctgggtggtt gacatctctg agtttcataa gccttatatt cgcccttttt aacggtaaca
     4021 atacgtaact ttaagcctaa ccgaaagctc cgaatggcca acatacttga tattaaa
//
LOCUS       DAOPMW010000304         4066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26079_length_4066_cov_6.663675, whole genome shotgun sequence.
ACCESSION   DAOPMW010000304 DAOPMW010000000
VERSION     DAOPMW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26079_length_4066_cov_6.663675"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLU11_12415"
     CDS             <1..885
                     /locus_tag="PLU11_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPN59978.1"
                     /translation="IGDRKERMLYAGLVVISANAGGAWSPIGDVTTTMLWIGGQITAA
                     NIMLKVFLPSMVSMLVPAFLISRQLKGNVSQRPEVLQNKNFTYSASERNLVFWLGVGC
                     LLMVPVFKTITHLPPYMGILFGLGIMWVCTEMIHSKKDEREKGVLSVNHALRKIDTPS
                     ILFFLGILLCVSALESTEILHAAAQWLDKTIGDISAISMTIGLLSALVDNVPLVAGAQ
                     GMYSLQQYPTDHYYWEFLAFCAGTGGSILIIGSAAGVAAMGLEKIDFFWYVKKIGWVA
                     LLGYLAGAAVFIAQQFLV"
     gene            complement(1104..1739)
                     /locus_tag="PLU11_12420"
     CDS             complement(1104..1739)
                     /locus_tag="PLU11_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPN59979.1"
                     /translation="MKPYEVLLHDNKHWAARKKAEDPAFFERLLHVQSPEFLWIGCSD
                     SRVPPDQITQTEPGEIFIHRNVANLVVNTDVNLMSVVDYAVNHLKIKHVVVCGHYGCG
                     GIKAAMSNTDFNQVLNMWLRNIKDIYRIHRKELEAITDEESRVNRMVEINVKEQIFNL
                     AKTAMIQRAWSNDQRPDLHGWVYDLHDGLIKPVIEMKAGADLDDLYEFEGL"
     gene            1958..2296
                     /locus_tag="PLU11_12425"
     CDS             1958..2296
                     /locus_tag="PLU11_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59980.1"
                     /translation="MAQRLSALLMILTLLWLTVSISFVYASMDNAGQDQAATSSLPAD
                     NEEESANPFGNNTEEKNPGSSSFSEEFLHHAESGSHFISSVIAFCTGENASLYVAFHG
                     ELLVPPPNCA"
     gene            2397..4013
                     /locus_tag="PLU11_12430"
     CDS             2397..4013
                     /locus_tag="PLU11_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPN59981.1"
                     /translation="MKKQQSLFANLKFDLPAGLVVFLVALPLCLGISLASGAPFFSGI
                     ISGIIGGVLIGALSNSPLSVSGPAAGLAALVLGAITSIGDFSLFLCAVFIAGILQMLL
                     GVAKLGGIANYIPGSVIKGMLTSIGILIIAKQIPHAFGYDKDEKGNITELFPYGQEDL
                     HELLQPLQHIEVGVFLLCLISIIIMVVWDMPFIKKRVSFVPGALIAVVVSVVINEIWI
                     KSGSQLAVMNEHLVQIKAASSASEFFSFFTLPDFNGFLNGSVIVYGIVIALIASLETL
                     LSIEAVDNLDPQRRVTNTNKELFAQGVGNTVAGLIGGLPVTSVIVRSSANVNSGGKSK
                     LSTIIHGSLLLVSIILIPGLLNKIPLSALAAILLLTGYKLAKPAVFKSFWTAGKFQFI
                     PFIVTVVVIIAVDLFGVLPALSGKGLLVGVLTGIVAAAGSILYGNLKNSYYFHSENHK
                     DGDMINIRLSEEVSFLNKAAIRQTLDHMPENSTVLIDASNTKYIDYDVLELIKEFRDI
                     KVPLKNIRLTLKGFKEAYNIENYSQVQSVH"
BASE COUNT         1018 a          913 c          966 g         1169 t
ORIGIN      
        1 attggtgacc ggaaagagcg gatgttatat gcaggactgg tagtgatatc cgccaatgcc
       61 ggaggcgcct ggtcaccgat aggagatgtg accaccacca tgctctggat cggcgggcag
      121 atcacggccg ccaatatcat gctaaaagta tttctaccca gtatggtgag tatgctggtg
      181 ccggccttcc tgatcagccg tcagttgaaa gggaatgtaa gtcagcggcc ggaagtactc
      241 cagaataaga attttaccta cagtgccagt gaaagaaacc tggtattctg gctgggagtg
      301 ggttgtttgc tgatggtacc ggtgtttaaa accatcaccc atctgccacc ctatatgggc
      361 attctttttg gattgggtat catgtgggtg tgtaccgaaa tgatccatag taagaaagac
      421 gaacgggaaa aaggggtttt atcagtcaat catgccctgc gtaaaattga tacgcccagt
      481 atccttttct tccttggaat attgttatgt gtttcagccc tggaatcgac tgaaatcctg
      541 catgccgcgg cccaatggct tgataaaaca attggcgata tttccgctat ttccatgaca
      601 atcggcctgc tttcggccct ggtggataat gtacccctgg tggccggtgc ccagggtatg
      661 tacagcctgc agcaataccc cactgaccat tattactggg aatttctggc cttttgcgcc
      721 ggtacaggtg gcagtattct gattattggt tcagccgcgg gagtagcagc catgggactg
      781 gagaaaattg attttttctg gtatgtcaaa aaaatcggat gggtggcgtt gctgggatat
      841 ctggcaggag cagcggtgtt tattgcgcaa cagtttttgg tgtagtggta agaaagaaac
      901 cggagttaaa gcttaagcag cgagcagcga gttacgagct tcgagaccgg tagttcgcta
      961 gcgttcttta tggctcgaaa ctcatagctc atacctcgta gctttttctg ctagttacga
     1021 gaagtgagct acgagcttcg agaccggtaa ttcgccctcg acctttatgt ttcgtacctc
     1081 gtacttcgta cctcgtactt tctctaaagt ccctcgaatt catacaaatc atccagatcc
     1141 gcaccggctt tcatttctat aaccggcttg atcagtccgt cgtggagatc atacacccag
     1201 ccatggaggt cgggacgctg gtcgttggac caggcacgct ggatcatggc ggtcttggcc
     1261 aggttaaaga tctgctcctt gacattgatt tccaccatcc ggtttacccg gctctcttca
     1321 tcagtgatgg cctcgagttc tttccggtga atccggtata tatccttgat attacggagc
     1381 cacatattga gcacctgatt gaaatcggta ttggacattg ccgctttgat cccgccacaa
     1441 ccgtaatgcc cgcataccac cacatgtttg attttcaaat ggtttaccgc atagtccacc
     1501 acgctcatca ggtttacatc cgtattcacc accaggttgg ccacattccg gtgaataaaa
     1561 atctccccgg gttcggtctg ggtgatctga tccggtggta cccggctatc actacacccg
     1621 atccacagga attccggaga ttgcacatga agcagccgtt caaaaaaagc gggatcttcc
     1681 gcttttttcc gggcagccca atgcttgttg tcgtgtaaaa ggacttcgta cggtttcatt
     1741 tttttagtta ggcttgaaag atttattcct gtttaatata ttgcaaaata atttgcggat
     1801 ttactaaaca aagtaaaggc gcaggtgttt tgacatttct gcgggttaaa cctgattaaa
     1861 attaaattaa tcttattgaa tttcaattcg tttggtaagg acatgtgaat acgctatttt
     1921 gcgaccccgg atgaaaaatg aacggaaaat acatacaatg gcacagcggc tttcagccct
     1981 gttgatgatc ctgaccttgc tttggctcac ggtcagcatt tcattcgtat acgccagtat
     2041 ggataatgcc ggtcaggatc aggccgcaac ctcctccctg ccagccgata atgaagaaga
     2101 atcggcaaac ccttttggga ataataccga agaaaaaaac cccggaagct cttcattttc
     2161 tgaagagttc ctgcatcatg cggaatcggg tagtcatttt atatcctctg tcattgcttt
     2221 ttgtacaggt gaaaatgcca gtctctatgt ggcattccac ggcgaactcc ttgttccgcc
     2281 tccaaattgt gcttaaaatc agctgattta ccttattgtt ctaaatgaac cgggcagcct
     2341 cattggcctg ccatttaatt actgtaaccg gtacaaacgt ctacttattt atatttatga
     2401 aaaagcaaca gtctcttttt gcaaacctga aatttgattt gcctgccgga cttgtggttt
     2461 tcctggtcgc cctgccactt tgtttgggta tatctctggc ttccggagcg ccttttttca
     2521 gtggtattat ttcaggtatc atcggggggg tcctgattgg tgcactgagt aattcaccgt
     2581 tgagtgtcag tggccccgcc gccggactgg ctgccctggt tctgggagca ataacatcta
     2641 tcggcgattt cagtttattt ctttgtgcag tttttattgc cggtatttta caaatgctgc
     2701 tgggcgtggc caaactggga gggatcgcga actatatacc cggaagtgtg atcaaaggga
     2761 tgctgaccag tattggtatc ctgatcatcg ccaaacaaat tccgcatgcg ttcgggtatg
     2821 ataaagatga aaaagggaat atcaccgagc tgtttccgta tggacaggaa gaccttcatg
     2881 aactgctgca acccctgcag catattgaag ttggtgtatt cctgctctgc ctgatttcca
     2941 tcattatcat ggtggtatgg gacatgcctt ttatcaaaaa aagagtaagc tttgtacccg
     3001 gtgcattgat cgctgttgta gtttcagtag tcatcaatga gatctggata aaatccggca
     3061 gtcagctggc ggttatgaac gaacacctgg tgcagatcaa agcggcttcc tctgccagtg
     3121 agtttttcag ctttttcacc ttgcctgatt tcaacggatt tctcaacggc tcagtcatcg
     3181 tatatggaat tgtgatcgcc ttgatcgcct ctctggaaac cctgctgagc attgaagccg
     3241 tggataacct ggatccgcaa agacgggtaa cgaataccaa taaggaatta tttgcgcagg
     3301 gagttggtaa tacggtggcc ggactgatcg gcggattacc agtaaccagt gtgatcgtcc
     3361 gaagcagtgc aaatgtgaat tcaggtggta agtcaaaatt gtctaccatc attcatggtt
     3421 cattgctgct tgtatcgatc atcctgatcc ccgggttgct gaataaaatt ccgttatcag
     3481 cgcttgcagc catcctgctg cttactggtt ataaactggc caaacctgcc gtttttaaaa
     3541 gtttctggac cgccggaaaa ttccagttca ttccgtttat cgttacggtt gtggtgatca
     3601 ttgcagtcga cttgtttggt gttttgcctg cactcagcgg caaaggactg ctggttgggg
     3661 tattgaccgg tattgtggct gcagccggtt ctattctcta tggcaacctg aagaattcct
     3721 attatttcca ttcagaaaat cacaaagatg gagatatgat caatatccgg ttatcggagg
     3781 aagtgtcctt tctgaataag gccgccatcc gccaaaccct ggatcatatg ccggagaatt
     3841 ccaccgtact gattgatgcg tcaaatacca agtatattga ttatgatgtt ctggagctga
     3901 tcaaagagtt ccgggatatc aaagtgccgt tgaaaaatat acggcttaca ctgaaaggat
     3961 ttaaagaagc gtataacatt gaaaattatt cacaagtaca atctgtacat taaaaaagaa
     4021 attataaaat gagagtacat actaaagaaa cacaatcaca cctgac
//
LOCUS       DAOPMW010000305         4060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26134_length_4060_cov_6.809738, whole genome shotgun sequence.
ACCESSION   DAOPMW010000305 DAOPMW010000000
VERSION     DAOPMW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26134_length_4060_cov_6.809738"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..3571)
                     /locus_tag="PLU11_12435"
     CDS             complement(173..3571)
                     /locus_tag="PLU11_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN59982.1"
                     /translation="MKKVKGLLIIVQLMLFPGYVFSQQPRFNKVLDVEKNGFGPVFSI
                     TQDQKGFVWFTSVLKGLQRYDGKKLTGFTHNNDNPNSLVNNLAVAVSVDSSGFIWVGT
                     IGFGVDRLDPETKKFTHFRHDPTDPSSLGSDTVFAIMTDRSGIIWIGSSRTLDKFNPA
                     TGKFTHYPIDGLVNYVPNSEEIPIVNTIIEDSNGFLWVGWGDPFTGKPDGTGGLSRFD
                     KSSGKFLHYKHDAGNPNSLSDNNVFCVFEDSKKVLWVGTKGNGLQSLDRATGKFTRYG
                     YYDPSHPVGLSRPPNSGSQFDYVSFVVEDIKGGLWTGSKSAGLNKFDPVQQKTTHFGR
                     VRNDKSNLFLKDTLDGFKDSSTFRAFSAKDGLLWIASEGSIYTVDFTSTFIPFTYLNF
                     PAGSFYFEEEKKNIWILTDSGMIRRNLLNNQQKVFRHDPRDANSLSDFSVFDLKGDEE
                     GNIWIATHNTGLEKFNIRTEQFTHIRHDKNNPASLVNDSSHVLFIDRQHFLWVGTHNG
                     LSRMDTKTGKCTNYIYDPADSLSIGKGHINCIIQDKSNRIWVGNDEDMFVLDSQSGKF
                     KRYKIGSGSLAMGCSDLDGVLWVACNAGLYFFDQQTDRFKKFTSQIFPEGIDGICGLV
                     EDEKGNLWVSTISNILRISRGRDEVKVYGTAQGVQSGTAVWMKNIRVNDGRLFLGGAK
                     GFYSFKPNELLDERTAPIINFTSFKIGSEEVVAGPGSPLKQPIWKTNEVRLNYSQNTF
                     SLEFGAIDYKNGGDIKYLCKLENYDIEWRDIGTEPKVTYFNLPAGKYTLYVKAINIDG
                     SVAEKSIAISIAPPWWKSWWAYTFYGLLVILAGFLIYKYQKSYIVKRERERTQQKELA
                     QAREIEKAYTQLKSTQAQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNTELIS
                     ELMEEVDKGNTAEVKSIATDIRENSEKIVHHGKRADAIVKGMLQHSRSSSNQKEPTDI
                     NALADEYLRLAYHGLRAKDKSFNATMHTDYDSSIGSIQIVPQDIGRVILNLITNAFYV
                     VNEKKALRQDQGNSYDPTVSVRTRKLDGKVEIKVSDNGNGIPQKVLDKIFQPFFTTKP
                     AGQGTGLGLSLSYDIVKAHGGEIKLETKEGEGTTFIIQLPDSLS"
     gene            complement(3579..>4060)
                     /locus_tag="PLU11_12440"
     CDS             complement(3579..>4060)
                     /locus_tag="PLU11_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN59983.1"
                     /translation="LRLAYHGLRAKDNSFNATMKTDFDPAIGNVNIVPQDIGRVILNL
                     ISNAFYAAPLPPKGGFSDPDYSHVPTVWVSTKREGDKVVISVRDNGPGIPAEIRDKIF
                     QPFFTTKPTGQGTGLGLSLSYDIVKAHGGELKVDTRTAGGDSVADTGTTFTIYLPVI"
BASE COUNT         1050 a         1012 c          818 g         1180 t
ORIGIN      
        1 aatcgcggtt ggtttcctcg taaaaagtat aaatggctct ggatacttta aactttacta
       61 aatccgctgt agcttgctga aaagcatacc gcaggctgtc aggcaacagg ggctgggcgg
      121 ttaatgtact tgagcaccca atcaataata ttataataac gaaacgtttc atttacgata
      181 atgaatcagg taattgaata atgaaggtgg ttccttcccc ttcttttgtt tccagtttta
      241 tctccccgcc atgtgccttg acaatatcat aacttaatga caaaccgagc ccggtaccct
      301 gcccggcggg cttggtggta aaaaacggtt gaaatatctt atccagcact ttctgtggaa
      361 tgccattccc attatcggac actttaattt ccactttacc atcgagcttc cgtgtcctga
      421 ctgaaaccgt tggatcataa ctattaccct gatcctgccg aagggctttc ttttcattta
      481 ctacataaaa agcattggtg atcaaattca aaatcaccct tccaatatcc tgcggcacaa
      541 tctgaatact tccgatgctt gaatcataat ccgtatgcat cgtcgcatta aaggacttat
      601 ccttggccct taacccatgg taagccagcc gcaaatattc atccgccagt gcattgatat
      661 ccgtaggttc tttctggtta ctgctgctgc ggctgtgctg cagcatgcct tttacaatgg
      721 catcggccct tttaccatgg tgcacgatct tctctgaatt ttccctgata tcggtcgcaa
      781 tagactttac ttcagcggta ttccccttgt ccacctcttc cattaattca cttattaact
      841 ctgtgttgac ttctgaaaaa ttattcacaa agttcaacgg gttttgtatt tcatgggcaa
      901 tgccggcggt aagttccccc aggctggcca ttttctccga ctgaataagc tgcgcctgtg
      961 tggacttcaa ttgcgtatag gctttttcaa tttccctggc ctgtgccagt tctttttgct
     1021 gggttctttc cctttcccgc tttacgatat aacttttctg gtatttgtag atgaggaatc
     1081 cggcaaggat cactaacaac ccatagaaag tataagccca ccaggatttc caccagggcg
     1141 gggcaatgct aatggcgatg cttttctccg ccacagagcc atcaatattg atcgctttga
     1201 catacaaggt gtatttacca gcggggagat taaaatatgt gacctttggc tcagtgccga
     1261 tatcccgcca ctcaatatca taattctcca gtttgcagag gtattttata tcgcccccgt
     1321 ttttataatc aatggccccg aattccaatg aaaaagtatt ttggctgtaa ttcagccgga
     1381 cctcattggt tttccagatt ggctgcttta acgggcttcc cggtccggcc accacctctt
     1441 cactcccgat cttaaaactg gtgaaattta taataggtgc tgttctctca tccagcagtt
     1501 cattgggctt aaagctgtaa aagccttttg ctccgccgag gaacaagcgg ccatcgttta
     1561 ccctgatgtt cttcatccat acagctgtac cggattgaac cccctgggct gtaccgtaca
     1621 ctttcacctc atcccgtccc ctgctgatcc ggaggatatt actaatggtg ctcacccata
     1681 agtttccttt ttcatcttcc acgaggccac aaatgccatc tattccctcc gggaaaatct
     1741 gggaggtaaa ttttttgaaa cggtccgttt gctggtcaaa aaaatacagg ccagcattac
     1801 aggctaccca taacactcca tccagatcgg aacatcccat ggccagtgaa ccactgccta
     1861 ttttgtagcg tttaaactta ccagactgac tgtccagcac gaacatatct tcgtcgttcc
     1921 ctacccatat ccggttactc ttatcctgta tgatacagtt tatatgtccc ttgccaatac
     1981 tcagactatc ggctggatca tagatataat tggtgcattt gccggttttg gtatccatac
     2041 gggacaaccc gttatgcgta ccgacccata gaaaatgctg ccggtcaata aacaggacat
     2101 gcgaggaatc attcaccagg ctggccggat tgtttttatc gtgccggata tgagtaaatt
     2161 gttctgtcct gatattgaat ttttctaaac cggtattatg agtggcgatc cagatatttc
     2221 cttcctcatc cccttttaaa tcgaacaccg aaaaatcaga aagactgttt gcgtcccggg
     2281 gatcatgcct gaatactttt tgctgattat tcaacaaatt tctcctgatc attccgctgt
     2341 cagtcaatat ccagatattc tttttctctt cttcaaaata aaatgagccc gcaggaaagt
     2401 tcagataagt aaaaggaata aacgtggagg taaagtcaac tgtataaatg ctaccctcgc
     2461 tggcaatcca gagtaaaccg tcttttgctg agaaagccct gaaagtggaa ctgtccttaa
     2521 atccatccag ggtatctttc aggaacaggt tcgacttatc atttctgaca cgtccgaaat
     2581 gcgtggtctt ttgctgcacc ggatcgaatt tattgagccc ggcagacttg gaaccggtcc
     2641 ataatcctcc ctttatatct tcgacaacaa aactgacgta atcaaactga ctcccgctat
     2701 tgggtggacg gctcaacccc accggatggg atggatcata atatccatac cgggtaaact
     2761 ttccggttgc ccgatccaga gactgtaacc catttccttt ggtaccaacc caaagtactt
     2821 ttttactgtc ttcaaaaaca caaaaaacgt tattatcaga caggctgttt ggattgcctg
     2881 catcatgctt ataatgtagg aatttgccgg aggacttatc aaatcgggat agcccaccag
     2941 tcccgtccgg tttaccggta aatggatctc cccagccgac ccataaaaaa ccgttactgt
     3001 cttcaataat ggtattgact atcggaatct cttctgaatt ggggacataa ttgacaagcc
     3061 cgtcaattgg gtaatgcgta aactttccgg tagccggatt aaacttgtca agggtccggc
     3121 tggaaccgat ccagatgatt ccagaacgat cggtcattat ggcaaaaaca gtatcacttc
     3181 ccagactgga aggatcggtt gggtcatgcc ggaaatgcgt gaattttttt gtttcaggat
     3241 caaggcgatc cacaccaaaa cctatggttc ctacccaaat aaaaccagat gaatccacgg
     3301 aaacagcaac tgccaggttg ttcaccaggg aattgggatt atcattgtta tgcgtaaagc
     3361 cggttagctt tttcccgtca taccgttgca atcccttcaa tacggaagta aaccagacaa
     3421 atcctttctg atcttgagta atggaaaaca ccggtccaaa gccatttttt tccacgtcca
     3481 gcactttgtt aaaccggggt tgctgtgaaa aaacataccc gggaaacaac atgagctgta
     3541 caatgataag tagcccttta acttttttca tttacgtttt atattacagg taaataaata
     3601 gtgaatgttg taccggtatc agccactgag tcgccacctg ctgtcctggt gtccactttc
     3661 aactcccctc catgcgcttt cacaatatca taactcagac tcaagcccaa ccctgttccc
     3721 tgcccggtcg gcttggtggt gaagaaaggc tgaaatattt tgtccctgat ttcggcagga
     3781 ataccaggtc cattatccct cactgaaata actactttat ccccttccct ttttgtactt
     3841 acccagactg ttggaacatg actataatcg gggtctgaga accccccttt ggggggcagg
     3901 ggggccgcat agaaagcatt gctgatcaga ttgaggatca cccggccgat atcctgcggt
     3961 acaatgttga cattgccaat tgccgggtca aaatccgttt tcatggtggc attaaaggaa
     4021 ttgtccttgg cccggagtcc atgataggcc agccgcaggt
//
LOCUS       DAOPMW010000306         4056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26168_length_4056_cov_5.886028, whole genome shotgun sequence.
ACCESSION   DAOPMW010000306 DAOPMW010000000
VERSION     DAOPMW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26168_length_4056_cov_5.886028"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLU11_12445"
     CDS             <1..1190
                     /locus_tag="PLU11_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN59984.1"
                     /translation="QHLPRLKELGVETLWFMPVTPIAQKNKKGSLGSYYACSDYTSVN
                     PEFGTMEDFKSLVSLAHQLGFKVILDWVANHTGWDHHWTVNHPGYYLKDTATNDFLKA
                     SGMDDIIELDFNNPALRKEMTAAMQFWVKECDIDGYRCDLASWVELDYWLEARPQVDA
                     VKPLFWLGEYDELEKPDYGKAFDASYSWGWMHLTEDFFKKQLPLDTLTRLLHQYDVLG
                     DSTMRAWFTTNHDENSWNGTEFEKYGSMFKALAVFSFTWNGIPLLYSGQELGNTKRID
                     FFDKDTIVAGPWAAELTGFYQSLIKLKSTNPALKAGDPSVTTYRLTTTDDRHVLAFLR
                     KKGNHEVLVVLNLSDQKDLHFEITGTEVTGLYKNAFSGAANDFTNERSFELQDWEFAV
                     YEK"
     gene            complement(1321..2826)
                     /locus_tag="PLU11_12450"
     CDS             complement(1321..2826)
                     /locus_tag="PLU11_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPN59985.1"
                     /translation="MKFKQLLLIVAVSAVSAVGSVWIYSKVVARQYTAAFNQSSDGKV
                     PVNYAGFLDNAAPLGEATDFTKAANAAVPAVVHIKTKTPAKKITNQLPRSRGNGNGFE
                     DLFEQFFNMGPQIQPEQRASGSGVIISDDGYIVTNNHVISDGGQGVAEEITVTLSNKK
                     TYKARVIGKDASSDIAVLKIDGTGFPFLLYGNSDNIKLGQWVLAIGYPLTLETTVTAG
                     IISAKGRSIGINGRQSETPIESFIQTDAAVNQGNSGGALITTDGQLIGINSAIYAPTG
                     TYAGYSFAIPVNLAKKIVNDLIKFGDVKRPYLGIAADNTKVNSTDGAYVGTVAPDGAA
                     ASAGLKKGDIIRKINDVNISSWTELQGTVASLNTGDKIAITYKRDGKEYTTSATLKAD
                     QGTYANLAANNLGDILGADLENLDKKTAIKNEVEGGVLVKKIRPGGAFSKTRMQDEFI
                     ITNVNGVEVKNIEDLGRALARSQSESVNLEGFYDGFDGMYRYPVKLSAGDE"
     gene            complement(2995..3993)
                     /locus_tag="PLU11_12455"
     CDS             complement(2995..3993)
                     /locus_tag="PLU11_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPN59986.1"
                     /translation="MNLQHRIYLLSRLGEYILGNSPDWRQVVEKAGYENGWFIPEFVD
                     SAVENIAKSYLSSEKLVAWADENHVPDRTNSPKKVGLIMAGNIPLVGFHDWLSVFISG
                     HYVVVKPSSRDSVLIRHLAGKIEAWDPAAATRTEFADMLKGCDAYIATGSNNSSRYFE
                     YYFSRYPHIIRKNRTSVAILNGKETADELEGLADDVYLYFGLGCRNITKIYVPEGYDF
                     VPLLEAFRKYSFLSDHHKYKNNFDYNLALHLLNKKYYMTNGSLLLVEEPALFSPISQL
                     NYEYYTEADKLTESLRNSGQVQSITGRGFVPFGQSQYPELADFADGINTLAFLNTI"
BASE COUNT         1046 a          918 c          941 g         1151 t
ORIGIN      
        1 cacaacactt gccccgttta aaagaactgg gtgtggaaac actctggttt atgccggtta
       61 cacccatagc gcaaaagaat aaaaagggaa gcctgggcag ttattatgcc tgttcggatt
      121 atacctctgt taatccggaa ttcggaacca tggaggactt taaatcgctg gtcagcctgg
      181 cccaccagtt gggatttaaa gtcatactgg actgggtcgc caatcataca ggctgggatc
      241 atcactggac ggtgaatcat ccgggttatt acctgaaaga caccgctacc aatgatttcc
      301 tgaaagcctc gggtatggac gatatcattg agcttgattt caataacccg gcgctgcgaa
      361 aggaaatgac agctgccatg caattctggg taaaggaatg tgatattgac ggttaccgat
      421 gcgacctggc ctcctgggtg gaactggatt actggctgga agcaagacca caagtggatg
      481 ccgtaaagcc gttgttctgg ctgggtgaat acgatgaact ggaaaagccg gattacggca
      541 aagcatttga cgcgagctac tcctggggct ggatgcatct gacggaagat ttttttaaga
      601 agcaattgcc actggataca ctgacgcgat tgctccatca atacgacgtc ttgggagatt
      661 cgaccatgag ggcctggttt accaccaatc atgacgagaa tagctggaac ggaaccgaat
      721 ttgaaaaata cggatccatg ttcaaagccc tggccgtatt cagttttacc tggaatggta
      781 ttcctttatt gtattccggc caggaactgg ggaataccaa acggattgat ttctttgata
      841 aggatactat tgtggctggc ccatgggcag cagaactcac tggattctat cagtctttaa
      901 tcaagctcaa atcgactaat ccggcattga aggcgggcga tccttccgtc accacttacc
      961 gcctgactac gaccgatgac cggcatgtgc tggcattctt acgaaaaaaa ggaaatcatg
     1021 aagttttggt tgtactcaac ctgtctgatc aaaaggacct tcatttcgag atcaccggta
     1081 ctgaagtaac cggcctgtat aaaaatgcgt tctctggagc ggccaatgat tttaccaatg
     1141 aaagaagctt tgagttgcag gactgggagt ttgcggtgta tgagaagtga gtggtgagtg
     1201 gtgagtgggg aataggaatt agggaatagg aattagggaa tagggaataa aggtgattac
     1261 aaataaaaaa ggcggtctga agttcaggcc gccttttttt tatgggtgag tcattcatta
     1321 ttattcatct ccggcactca gcttaaccgg gtagcggtac atgccgtcaa atccatcata
     1381 gaatccttca aggttcacgc tttcactttg actgcgtgcc aatgccctgc caaggtcttc
     1441 tatattttta acttccacgc cgttcacatt ggtaataata aattcatcct gcatccgggt
     1501 tttgctgaag gcaccacctg gcctgatttt cttaaccagt actccgcctt ccacttcatt
     1561 tttaatcgcg gttttcttat ccagattttc aaggtctgcc cctagtatat ctcccaggtt
     1621 attggctgcc aggttagcat aagtaccctg atccgctttc agtgtggcac tggtggtata
     1681 ttcctttcca tctctcttat aggtgatggc tattttatcc cccgtattca gggaggctac
     1741 ggtgccttgt aattcggtcc aggaactgat attcacatca ttgatcttgc ggatgatatc
     1801 tccttttttc agaccggcag aagcggctgc accatcagga gcaactgtac caacataggc
     1861 gccgtctgtg gaattaactt tggtattatc agctgcaata ccaaggtatg gtctttttac
     1921 atcgccgaat ttgatcaggt cattgacaat ctttttggcc aggttgactg gtatggcaaa
     1981 cgaataaccg gcataagtcc cggtaggggc ataaatagcc gaattgatac cgatcagttg
     2041 tccatccgtg gtaatcagtg caccaccact gtttccctgg tttacagctg catcggtctg
     2101 tataaaggat tcaatcgggg tttcactctg acgtccattg attccaatcg aacgtccctt
     2161 agcactgatg ataccggctg taacagtggt ttcaagggtc agaggataac cgatcgccag
     2221 tacccattgt cccagtttta tgttatccga gttgccataa agcaggaagg ggaagccggt
     2281 gccatcaatt tttaacacgg ctatgtcgct gctggcgtct ttgccgatta cccgggcttt
     2341 gtatgttttt ttattactca gggttacggt gatttcttca gccacccctt gtccgccatc
     2401 tgagatgacg tgattgttag tgacgatata tccatcatcg ctgataatga ctccgctgcc
     2461 ggaggcacgc tgctctggtt ggatctgcgg tcccatattg aaaaactgct caaacaggtc
     2521 ttcaaaacca tttccattac cacggctgcg gggcaattgg ttggtgattt ttttggccgg
     2581 cgtttttgtt ttgatatgta ctacggcagg tacggctgca ttggctgcct tggtaaaatc
     2641 cgtggcttcg cccaacggag cagcattgtc gaggaaaccg gcataattga ccgggacctt
     2701 tccatctgaa gactggttaa acgcggctgt atattggcgg gcaaccactt tactgtagat
     2761 ccacacactg cctacagcgg aaactgcact gactgcaacg atcaatagaa gctgtttaaa
     2821 tttcatagtg ctttttattt ttttcagttt aaattttaca aatcctgtgc ctttgacgta
     2881 caaaataaca aactgtcagt agttctttgt ttaccattgg gttagtttaa caaatcttta
     2941 ctaaagcctt cgtactttgg ccatccggct gtaatttata taaacaattc agattcagat
     3001 tgtgttcaga aaagccaggg tattgattcc gtcagcaaaa tccgccaatt caggatactg
     3061 actttgtcca aaaggtacga aacccctgcc ggtaatagat tgaacctgac cggaattcct
     3121 gagcgattcc gtcaatttgt cggcctccgt ataatattcg taattcagct ggctgatcgg
     3181 ggagaaaagc gcgggttctt ccaccaacag cagggagcca ttggtcatgt agtatttttt
     3241 attgagcagg tgaagggcca ggttatagtc aaaattgttt ttgtacttgt ggtggtcgga
     3301 aaggaaactg tatttcctga aagcttccag caaaggcaca aaatcatacc cttcgggaac
     3361 gtatattttt gtgatgttcc ggcaacccag gccgaaatac aagtacacat catctgccag
     3421 tccttccagt tcgtcagccg tttccttgcc attcaggatt gcaacggaag tcctgttctt
     3481 ccggattata tgtgggtacc tgctgaagta atattcaaaa taccgggagg aattattact
     3541 gccggtagca atgtaggcat cacatccttt caacatatcc gcaaattccg tccgggttgc
     3601 tgctgcagga tcccatgctt caattttacc ggccaggtgc ctgatcagca cactatcccg
     3661 ggatgaaggt tttacaacca cataatgccc gctgataaaa acggaaagcc agtcgtgaaa
     3721 accaacaagc gggatattgc cggccataat taagcccact tttttggggg aattagtacg
     3781 atcggggaca tgattttcgt cagcccaggc aaccagtttt tcactgctta aataggattt
     3841 tgcaatgttt tccaccgctg aatccacaaa ttcgggtata aaccagccat tttcgtagcc
     3901 cgctttttcc acgacctgac gccagtcagg tgaattgccc aaaatatatt caccaagcct
     3961 tgataataaa taaattctgt gttgtaaatt catccctgtc tactaatttt gtaaggcaaa
     4021 agtaaccctg aacccgttaa tttagtttat tatggc
//
LOCUS       DAOPMW010000307         4040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26304_length_4040_cov_6.160853, whole genome shotgun sequence.
ACCESSION   DAOPMW010000307 DAOPMW010000000
VERSION     DAOPMW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26304_length_4040_cov_6.160853"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1667
                     /gene="gpmI"
                     /locus_tag="PLU11_12460"
     CDS             141..1667
                     /gene="gpmI"
                     /locus_tag="PLU11_12460"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPN59987.1"
                     /translation="MSSKKVILVIMDGWGLGKVASADAIQHAKTPFTNSLYHQFPHTT
                     LITCGEDVGLPEGQMGNSEVGHLNLGAGRIVYQELQRINVAVRDGSFSQNETLLKSIR
                     FAKANNKPLHLLGLVSNGGVHSHINHVKALVDVCKSEGLEKVFIHAFTDGRDCDPKSG
                     LAFIIELQQHLNGSVGKIASVSGRYYAMDRDKRWERVKLAYDALVKGEGAKATDAVAA
                     VENAYAANITDEFIKPTVIVDEAQQPLATIQEGDVAICFNFRTDRCREITQVLTQLDL
                     PEFDMHKLSLHYTTMTEYDKTYKEVHVVFENDNLNNTLGEILEQNGKKQIRIAETEKY
                     PHVSFFFSGGREKPFEGEKRIMVQSPKVATYDLQPEMSAHEITDAIVNEINIQSADFI
                     CLNFANADMVGHTGIWEAAIKAVETVDHCVERVVTAGLANGYTIFLTADHGNSDFMIN
                     EDGSPNTAHTLNPVPFFIIDKEWKGKVKPGKLGDLAPTILTMMQLPIPKEMTGEVLID
                     "
     gene            1685..2173
                     /locus_tag="PLU11_12465"
     CDS             1685..2173
                     /locus_tag="PLU11_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HPN59988.1"
                     /translation="MFKKIMLVLLAALVVIQFIHPKKNTSEGPQPNYIGNNYAVPTDV
                     KSILVKACNDCHSNNTRYPWYAKIQPVDWWLNTHVLDGKKEINFDEFSNRAPRYQYRK
                     MEEVIEMVKEREMPLKSYTWTHKDARLTSIEREKMTGWAQSVMDAMKVKYPVDSLIRP
                     KR"
     gene            2187..2705
                     /locus_tag="PLU11_12470"
     CDS             2187..2705
                     /locus_tag="PLU11_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59989.1"
                     /translation="MRRNRNRPLISLLLLLPVLIPLLFIVMNDLNKWQIKYQGRERAK
                     SSASLVQLRMHESKVQWMDKHEIFVNGRMFDITKSELNNGWYHFTGHYDDPETKLLRK
                     QQKAQQQKDGQQTLLQVFKSLQQLYCESQEPGFLPATASLFQPYIPNDRLPAASLEVS
                     TPPPQISHLTTL"
     gene            2735..>4040
                     /locus_tag="PLU11_12475"
     CDS             2735..>4040
                     /locus_tag="PLU11_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN59990.1"
                     /translation="MRRALLLCVYLYACIPVFAQSDTLNEKTMDEAVVSVNKWEQKLN
                     EVPNKIIKVSKYDILRNNPQTSADLLGQSGTVFIQKSQLGGGSPMIRGFATNRVLLVV
                     DGVRMNNAIYRSGNLQNIISIDPLSTEAAEVIFGPGSLIYGSDAIGGVMDFHTLEPRF
                     SKTDKMQAKGAVLGRYSSANLEKTGHADLNLGWKKWSLLTSVSYSRFDDLKMGKNGGQ
                     DSYLRPEYVKRINNKDSIVKNSDPRIQRFSGYDQLNFLQKIRFKPGQYWDLLYSFTHA
                     KTGNAPRYDRLIQYRNGALRFAEWYYGPMIWDMHTLNVLHTKKTVWYDDVRLTVAYQD
                     YEESRIDRTRANNNRNKQTELVDAISVNLDANKSLVKGQLFYGLEYVHNKVGSLGERT
                     NISTGVVSPMVSRYPNNSTWSTTGIYGSYKINLNPKLTLTTGL"
BASE COUNT         1206 a          914 c          964 g          956 t
ORIGIN      
        1 caccggtact aaaatactac aaatcaggtg cttttaccaa atcagggtgg ataatagctg
       61 aattgatgta gttttacagg gtacggatgg gaaatcttcc ctgattttta ttgtgataac
      121 cccttgaata attgagtcaa atgtcatcaa aaaaagtaat cctggtcatt atggacggtt
      181 ggggactggg aaaagttgca tccgccgacg cgatccaaca cgccaaaaca ccgtttacca
      241 acagtttata tcaccaattt ccccatacca cgctgattac ctgtggtgag gacgtagggc
      301 tgcccgaagg gcaaatgggg aactctgaag taggtcacct gaacctcggc gccggtcgga
      361 tcgtgtacca ggaattgcag cggatcaatg tggcagtcag agatggttcc ttttcacaga
      421 atgaaaccct gttgaaatct atccgctttg ctaaagccaa caataaaccc ctgcacctgc
      481 tgggtctcgt gagtaatgga ggcgtacact cgcatattaa tcatgttaaa gcgctggtgg
      541 atgtgtgtaa atccgaagga ctggaaaaag tattcattca tgcatttact gacggccgcg
      601 attgtgatcc gaaaagcggg ctggccttta tcatcgagtt gcagcagcac ctcaatggaa
      661 gtgtgggaaa aattgcgagt gtaagcggtc gctactatgc gatggaccgg gataaacgct
      721 gggaaagggt aaagctggct tatgacgccc tggtaaaagg agaaggagcc aaagccaccg
      781 atgcagtagc cgctgttgaa aatgcctatg ccgcgaatat tacggatgaa ttcatcaaac
      841 ccaccgtcat tgtggatgaa gcacagcaac ccctggccac catacaggaa ggagatgtgg
      901 cgatctgctt caatttccgc accgaccgtt gccgtgaaat cacccaggtg ctgacccagc
      961 tggatctgcc ggagtttgat atgcataaac tgtcgctgca ctataccacc atgacagaat
     1021 atgataaaac ctacaaagag gttcatgtcg tttttgaaaa tgacaacctg aataatacac
     1081 tgggagaaat tctggaacag aacggaaaaa aacagatccg cattgccgaa acagaaaaat
     1141 acccgcacgt cagtttcttc ttcagtggtg gccgtgaaaa accgtttgag ggtgaaaaaa
     1201 ggattatggt tcagtcaccg aaggttgcga catatgacct gcaacctgaa atgagtgcgc
     1261 atgagatcac cgatgcaatc gtgaacgaaa ttaatatcca gtcggctgat tttatctgcc
     1321 tgaactttgc caatgcggac atggtgggac ataccggtat ctgggaagcg gcaatcaagg
     1381 ccgttgaaac ggtggatcat tgtgtggaaa gggtggtaac cgccggactg gccaatgggt
     1441 atacgatctt cctgacggcc gatcatggca actctgattt tatgatcaat gaagacggca
     1501 gtccgaatac ggcccatacc ctgaacccgg tccccttctt tattatcgat aaagaatgga
     1561 aaggcaaagt gaaaccgggt aagcttggcg acctcgcccc tacgatatta acgatgatgc
     1621 aattgcccat cccgaaagaa atgacgggag aggtacttat agactaacct caaaaacacc
     1681 acgtatgttc aagaaaatca tgcttgtttt actggccgca ctggttgtca tccagtttat
     1741 tcatccgaaa aaaaatacca gtgagggccc gcagcccaac tatatcggga ataactatgc
     1801 ggtgcccacg gatgttaaat ccatcctggt taaagcctgc aatgattgtc acagtaacaa
     1861 cacccgctac ccctggtatg caaaaatcca gccggtggac tggtggctga atacgcacgt
     1921 attggatgga aaaaaagaaa tcaactttga tgaattcagt aaccgggcac cccgttatca
     1981 atatcgtaag atggaagaag taattgaaat ggtgaaagaa agggagatgc ccctgaagtc
     2041 ctatacctgg acccataaag atgccaggct gacttctatt gaaagagaaa aaatgaccgg
     2101 ctgggcacaa tccgttatgg atgccatgaa agtgaaatac ccggttgaca gcctgataag
     2161 gccaaagcga taatccgttt ttgcacatgc gaagaaacag aaaccgacca ctcataagcc
     2221 tcctgctgtt gctgccggta ttgattccgc tgttgtttat cgtcatgaat gacctgaaca
     2281 aatggcagat caaataccag ggccgtgaac gtgcgaaatc atcggcatct ctggttcaac
     2341 tgcgcatgca tgaaagtaaa gtacaatgga tggacaaaca tgagattttt gtgaacggcc
     2401 gcatgtttga tattacaaaa tcagaattaa ataacggctg gtaccatttt accggccatt
     2461 atgatgaccc tgaaaccaaa ttgctgagaa aacaacaaaa ggcacagcaa cagaaagacg
     2521 gtcagcaaac acttttacag gtatttaaat ctttgcaaca actttattgc gaatcgcagg
     2581 aacccggatt tctgccggct actgcttcgc tatttcaacc gtacataccc aatgaccggc
     2641 tacccgccgc cagcctggag gtaagtactc ctcccccgca gatcagccat ctaactactc
     2701 tttaatcgca ttttttttac gtaaactgat ctttatgaga agagcgttac tgctttgcgt
     2761 atacctgtat gcgtgcatac cggtatttgc gcaatcagat accctaaatg aaaaaaccat
     2821 ggatgaagcc gtggtttcgg ttaataaatg ggaacagaag ctgaacgagg ttcccaataa
     2881 aattatcaaa gtcagcaaat atgatatcct gagaaacaat ccgcaaacat cggccgacct
     2941 gctcggtcag agcggcactg tgtttattca gaaaagccag ttaggtggtg gcagcccgat
     3001 gatccgggga tttgctacaa accgggtatt actggtcgtt gatggcgttc ggatgaacaa
     3061 cgccatttac cggagtggca acctgcaaaa tataatcagt atagatccat tgagtacaga
     3121 agcggccgaa gtgatattcg gtccgggttc cctgatatat ggcagtgatg ctattggcgg
     3181 tgttatggat ttccataccc tggagccccg tttttcaaaa acagataaaa tgcaggccaa
     3241 aggggctgtg ctgggcagat actcttcggc caacctggaa aaaaccggtc atgccgacct
     3301 gaatctcggc tggaaaaaat ggtccctcct tacttccgtt tcatacagtc gttttgatga
     3361 cctgaaaatg ggaaaaaacg gcggtcagga cagttacctg cgcccggaat atgtgaaacg
     3421 cattaataac aaggatagta ttgtaaaaaa cagtgatccc cgtattcaac gcttcagcgg
     3481 gtatgatcaa cttaattttt tacaaaaaat tcgtttcaaa ccgggtcagt actgggatct
     3541 gctgtacagc tttacccatg ccaaaacagg gaatgctccc cgttatgacc ggctgattca
     3601 gtaccgcaac ggggccctgc gttttgcaga atggtattat ggtccaatga tctgggatat
     3661 gcataccctg aatgtattgc atacgaaaaa gaccgtatgg tatgatgatg tccggctgac
     3721 ggtagcctat caggattatg aagaaagccg gattgaccgt acccgtgcaa ataataaccg
     3781 gaataagcag acggaattgg ttgatgcgat cagtgtcaac cttgatgcca ataaatcact
     3841 tgtaaaaggc caactttttt atggtctgga atacgtgcat aataaagtgg gctcgctggg
     3901 cgaaagaacc aatatcagca ccggggtggt cagccccatg gtcagccgtt accccaataa
     3961 cagcacctgg agtactacag gcatttacgg cagctataaa atcaatctga acccgaaact
     4021 gaccctgacc accggacttc
//
LOCUS       DAOPMW010000308         4039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26312_length_4039_cov_8.098394, whole genome shotgun sequence.
ACCESSION   DAOPMW010000308 DAOPMW010000000
VERSION     DAOPMW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26312_length_4039_cov_8.098394"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLU11_12480"
     CDS             complement(<1..414)
                     /locus_tag="PLU11_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59991.1"
                     /translation="MKKIILVFIAALLFANQLTAQHCPFDGGHIVVIKLTDAHGNIIT
                     TADSTLFLTETPNPLADSCTYAKGQLNRPFDRPEKNLVKKYEGSWEYRAALYLEECTF
                     KEPGHLVVVLNMAQRSCMIERNNQFTYLERHFEIHQ"
     assembly_gap    1146..1245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1260..2366
                     /gene="carA"
                     /locus_tag="PLU11_12485"
     CDS             1260..2366
                     /gene="carA"
                     /locus_tag="PLU11_12485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPN59992.1"
                     /translation="MPNSSIPAVLLLADGTVHHGQAFGAIGTTTGEICFNTGMTGYQE
                     VFTDPSYFGQILIMNSAHIGNYGVKDGDVESDSVKINGLIGRNLEDQYSRKMANGSLN
                     EYLKTNKVVSIEGVDTRALVAQIRTKGAMNCIISSETTDIEVLKKKLAETPNMDGLEL
                     ASKVSTVSSYELGDAASGLRIAVMDYGVKKNILQCMVDRGAHVKVFPAKTPLEEVKKF
                     QPDGYFISNGPGDPAPMDYAVATVKQILKEEKPLFGICLGHQLLALANDIPTFKMHHG
                     HRGLNHPVKNLISGRSEITTQNHGFGVDPEAVKKAEHIEITHLNLNDQSIEGIRVKGK
                     PAFSVQYHPEATPGPHDSRYLFDEFLELVKAHKN"
     gene            2549..3553
                     /locus_tag="PLU11_12490"
     CDS             2549..3553
                     /locus_tag="PLU11_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPN59993.1"
                     /translation="MHHRLVRHLLCCFLLIYQWLPVAAQSPAKYKEIDAFIKADQLKV
                     KSLADIKVFGRELKRLFTDDEIKARAAFFWLSQHIKYDCEGYRSRRYTADPAEVLSKG
                     IAVCSGYARLMKMFCDELGVECEVVSGFATGISVDIVHLDSMESNHAWNAVKIKGEWK
                     LVDPTWGSGSANNDCTKTYPALNEQYFLSDPRFMIQSHLPEEEKWQLISAPYSARQFA
                     DSANARENASKWAEPRDSVIKRRVGDIIRFRLLKEDLRNFFSVMLYNKEDSLIEDLNV
                     PVKLSADGFYYCDYKVKRAGAYDICLSLYYFTGKETDFVGTSIVSYKLLVNPATSKQP
                     "
     gene            complement(3559..3981)
                     /locus_tag="PLU11_12495"
     CDS             complement(3559..3981)
                     /locus_tag="PLU11_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59994.1"
                     /translation="MTFLEALYGSQYHEIQQRGGDGSKARFNGNLLLAVMVLLGIMIF
                     FLAAIKFVPGFNESITRQVRGLFGYSSGKTIGRLLALPFFFLLFWILQQTTGKAANFK
                     KYVDGFLAYPEEIKKQGNKKILFPFFILLGIVILLVLA"
BASE COUNT         1112 a          796 c          971 g         1060 t
ORIGIN      
        1 ctgatgtatt tcaaagtgac gctcgagata ggtaaactga ttgtttcgtt ctatcatgca
       61 gctgcgctgg gccatgttca gtaccactac taaatggccg ggttctttga acgtacattc
      121 ttcgaggtaa agggcggctc tatactccca ggagccttcg tattttttta ccaggttttt
      181 ttcggggcgg tcaaaaggac ggttgagttg tcctttggcg taggtacagg agtcggctaa
      241 cgggttgggg gtttctgtca gaaaaagcgt gctgtctgct gtagtaataa tatttccatg
      301 ggcatcggtc agttttatga caacaatatg tcctccatca aagggacaat gctgcgcggt
      361 tagctgattg gcgaacagga gtgctgctat aaataccagg ataatctttt tcataagggg
      421 catgcaggtt taaatgatca gaaccgaaag taaaaaatta ttgatcatga tcactaccct
      481 gtttacggta ttttctttta acggatatat attgagatta ttcttgttgc gccaaatcaa
      541 tgattttttt tagcggcgtt tacaccgaat gttaaatccc tttaacttta cgctgatcct
      601 gcaaggggtc gcaccaaaac taacagcgag aaaagcgatc caatatccat ttaaaaagat
      661 cagtagttaa ggttcattat cgaaaccatt tagcggccgc ctcggttggt gataacacca
      721 accgatggcg gtcgttcttg ttggtgttat caccaacaag aagtgttggt gataacacca
      781 acacttgaca gtgggctgga gataacacca actcttgaca gtgggttgga gaaaacacca
      841 acccttagcg gagtctgcgg aactaatttt tgccgtagac tgtaatcagg tagttttcga
      901 tactgattag tttcgggccc gggaagctgt tgatcccccc ttcgctaaag cttcggggga
      961 taaatcggaa gggtaaaatg taaaacgtga attgtatttg gaaaacacgt agctttacaa
     1021 atcatcatgc attaatggca agatgataca acgaattgat ctaatatcca tccaaagact
     1081 gtgttaaggt tcaaatacga actttttcaa aagcggcatc tgaaaagatg tcgctttgct
     1141 tttagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaaag ccctgaaaaa
     1261 tgcctaattc ctccattccc gctgttttat tgttagccga cggaaccgtg caccatggcc
     1321 aggcttttgg cgccatcggg acgaccactg gcgagatctg ttttaatacc gggatgaccg
     1381 gttaccagga agtgtttacc gaccccagtt attttggcca gatcctgatc atgaacagtg
     1441 cgcatatcgg taactacggg gtgaaagatg gggatgtgga atcggacagt gtgaagatta
     1501 atggcctgat cggccggaac ctggaagacc agtattcccg caaaatggcc aatggttccc
     1561 tgaatgaata cctgaaaacc aacaaggtag tatccattga aggggtggat acccgggcac
     1621 tggtggccca gatccggacc aagggtgcta tgaactgtat catttcttcc gagacaaccg
     1681 atattgaagt attgaagaaa aagctggccg aaacccccaa tatggacggg ctggagctgg
     1741 cttccaaagt aagcactgtt tccagttatg aactggggga tgcagcttcc ggactccgga
     1801 tcgcggtgat ggactatggc gtgaaaaaga atatcctcca gtgtatggtg gaccgcgggg
     1861 cacatgtaaa agtattcccg gccaaaaccc cgctggaaga agtaaaaaaa ttccagcccg
     1921 atggctattt tatctccaat ggtcccggcg atccggctcc catggattat gcagtagcta
     1981 ccgttaaaca gatcctgaaa gaagaaaagc ccttgtttgg tatttgcctg gggcaccagc
     2041 tcctggccct ggccaatgat attcccacgt tcaaaatgca tcatggtcac cgtggtctca
     2101 atcacccggt gaagaacctg atcagtggac gctcggagat cacgacccag aaccatggtt
     2161 ttggcgtaga cccggaagct gtgaagaaag cagaacatat tgagatcaca cacctcaacc
     2221 tgaatgacca gagcatcgaa ggcatccggg taaaagggaa gcccgcattt tccgttcaat
     2281 accatccgga agccacaccg ggtccgcatg attcaaggta tttgtttgat gagttcctgg
     2341 agctggtgaa ggcgcataag aattagttgg aggttggagg ttggaggttg gaggttggag
     2401 gttggaggtt tcgtgagaag ggaaaaggta caaagcttgc aatcatgctg acagagggaa
     2461 atattgattt aatagcttgt taatactgta tcaaatgagt aaaaagccgc tttttagaga
     2521 gcggtttttt ttatttttag gatacactat gcaccaccgc cttgtccgtc atcttttatg
     2581 ctgttttctt ttgatttatc agtggcttcc ggttgccgcg caaagtccgg cgaagtataa
     2641 agaaatcgat gcttttatta aagcggatca gttaaaagta aaatcactgg ctgatatcaa
     2701 agtgtttggc agggagctga aacgattatt taccgatgat gaaataaaag ccagggctgc
     2761 ttttttctgg ttatcccagc atattaaata tgattgtgag ggatacaggt ccaggcgtta
     2821 tactgctgac ccggcagaag tattatcaaa aggtatagct gtttgttccg ggtatgccag
     2881 gctcatgaaa atgttctgtg atgaattggg cgttgagtgt gaagtggtca gtggatttgc
     2941 caccggaatt tctgtagata ttgtgcacct tgatagcatg gagtcaaacc atgcatggaa
     3001 tgcagtaaaa attaaagggg aatggaaact ggtggatcct acctggggaa gcgggagtgc
     3061 aaataatgac tgtactaaga catatcctgc tttaaatgag caatatttcc tctctgatcc
     3121 caggttcatg atacagtccc atttgccgga ggaggaaaaa tggcagttga tttctgcccc
     3181 ctattctgcc cggcagtttg ctgattcggc gaatgccagg gaaaatgcat caaaatgggc
     3241 agaaccccgt gattctgtaa ttaaaagaag ggtgggagat ataatacgat tcaggctgct
     3301 aaaagaagat ctccggaatt tcttttcagt tatgttatac aataaagaag acagcctgat
     3361 cgaagacctg aatgttccgg taaaactgag tgccgatgga ttttattatt gtgattataa
     3421 agtaaaacgg gccggtgctt acgatatatg tctgtcactc tattacttta ccggtaaaga
     3481 aaccgatttc gtgggaacaa gcattgtttc ttataagtta ttggtgaatc ctgcaactag
     3541 taaacaaccc tgataaagtt atgccagtac cagcaggatt acgattccca acaatataaa
     3601 aaaaggaaac agtattttct tattcccctg ctttttaatc tcctccggat aagccaaaaa
     3661 tccatccaca tattttttga aatttgccgc tttgccggtt gtttgctgca gaatccaaaa
     3721 gagcaggaaa aagaagggga gggccagtag ccggccgatg gtcttacctg aactgtagcc
     3781 aaaaagcccc ctgacctggc gggtgatgga ttcgttgaaa ccgggtacaa actttatcgc
     3841 tgccaggaaa aagatcatta tgcctaaaag taccatgacg gccagtaaga ggttcccatt
     3901 aaaacgggct ttactgccgt ctccaccgcg ttgctggatt tcgtgatact gactaccgta
     3961 aagggcttcc aggaaggtca taaacagatg ttgattattt ggattatttt cgggaaatga
     4021 agatagcaat tatcaacgg
//
LOCUS       DAOPMW010000309         4034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26365_length_4034_cov_10.617743, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000309 DAOPMW010000000
VERSION     DAOPMW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26365_length_4034_cov_10.617743"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLU11_12500"
     CDS             <1..227
                     /locus_tag="PLU11_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59995.1"
                     /translation="DSTWYNVWLELEAGIQSNDKKDTLYSRQLNGSLLHIDQIKKDSI
                     AVGNTYKFVVQDIISGSCTPRVTMFRFEKF"
     gene            237..1166
                     /locus_tag="PLU11_12505"
     CDS             237..1166
                     /locus_tag="PLU11_12505"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPN59996.1"
                     /translation="MPIASTLLMVRPAAFGYNDETAANNFFQHHPGLDRTTLQQKALT
                     EFDAMVDLLRSHQVELLVLEDSKEPPKPDAVFPNNWISTSPDGKIFIYPMFAPNRRTE
                     KRDELLEQITREYVVVDVQDWSEFEAEGRFLEGTGSMVIDYDHEMIYAAISERTNLSV
                     LEKFATANGFQAIVFLATDKEGQPIYHTNVVMALGEKFAVLCEEAIEEEWELIAVKQL
                     LESTDHTIIPITRDQLYAFAGNMLEIKNKKGESLIVLSETAMSALRPDQIQQLQSFAT
                     LIPVAVPTIEQVEGGSVRCMLAEIFLQRKIKEG"
     gene            complement(1153..2208)
                     /gene="selD"
                     /locus_tag="PLU11_12510"
     CDS             complement(1153..2208)
                     /gene="selD"
                     /locus_tag="PLU11_12510"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPN59997.1"
                     /translation="MQDPVRLTQFSHGAGCGCKISPKVLDEILKTSVAFPDNDKLLVG
                     NHSRDDAAVYDLGNGTAVISTTDFFMPIVDDPFEFGRIAAANSISDVYAMGGKPLMAI
                     AVLGWPVNKLPPDVAQKVVEGGRSICLEAGIPLAGGHSIDSPEPIFGLAVTGILPIEN
                     LKQNNTAKEGDLLFLTKPLGVGILSTAQKRGLITDELLAVIIKQMTTLNKTGEELGKI
                     KGVHAMTDVTGFGFLGHLIEMAEGSGLSAEVWYDKIPAIPGVKEYIAQGVFPDGTTRN
                     WSSYGEKVKFEKGTPVAEAFKLLPDPQTNGGLLIAISPEAKDELIGILEKNGLGGFIT
                     PLGQMKAKGEKAVVILP"
     gene            complement(2295..>4034)
                     /locus_tag="PLU11_12515"
     CDS             complement(2295..>4034)
                     /locus_tag="PLU11_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN59998.1"
                     /translation="WLSFLFLLAPLFFYGQTLTGLWVGALHNDSSSVRKDQSFEIALT
                     EYRGKVFGYSRSEFIVNDTLYYIVKRVKGTIEGDICEVKDDEIIAYNFRGKLDKGIKV
                     TSTFRRNQTDSTWQLDGSWKTNATKKYYSVTGKVNLAEEKDLTASKLFPHLEELNKAD
                     EIAFYKERTEPAPFVKVAKPERIQSEYTEKADILAKNEEKSLSVKKPELPQPAQPVVP
                     EVAVQSVPETKTTPVLPADALPGNNPVATNNQAQEKPGVKNEPALTAVPEKKNPDPQT
                     QGKLTAKAKTETNIPVVPVKTETPPVKTAAPANSANETANTSTANPEVAAVKPAPATS
                     PVVKTQPATPPVTTATVKPSPSQEEKAVVKTTNPSAKNQQTDAPKTIVATKAPEEKIA
                     AVAPENLAKTKPATATVVNNKPAATEPALTPVSNKAVIANPVETNLPKPRVIDPTALL
                     VKKSVEDPIKKSTMIAGRKSEFNQEVYFKSDSLILTLYDNGEIDGDTVSVYLNGEVVM
                     PSQGLKSSAIRKTIHMDDGLETFTLVMFAESLGKYPPNTGLLVIRDGNDVYNLRFSSD
                     FQKSSGIVFRRFK"
BASE COUNT          942 a          921 c         1017 g         1154 t
ORIGIN      
        1 cggacagcac ctggtacaat gtatggctgg aactggaagc cggtatccaa tctaatgata
       61 agaaggatac cctgtattca agacaattaa acggaagtct gctgcatatt gaccagataa
      121 aaaaagacag tattgctgtg gggaatactt ataagttcgt ggtacaggat attatcagcg
      181 gaagctgtac tccccgtgtt acgatgtttc gctttgaaaa attttaattt catcatttgc
      241 caatcgcatc tacattgtta atggtgaggc cggccgcatt cggctataac gacgaaacgg
      301 ctgccaataa tttcttccag catcatcccg ggctggaccg cactacgctg caacaaaaag
      361 ccctgactga atttgatgca atggtggatt tgttgcgcag ccatcaggtg gaactgcttg
      421 tgctggaaga cagcaaggag ccacccaaac ccgatgccgt atttcccaat aactggatca
      481 gcacatctcc cgacggtaaa atatttattt atcccatgtt tgcgcctaac cggcgtacag
      541 agaaaaggga cgagttgctg gaacagatta cccgtgagta tgtggtcgtc gatgtgcagg
      601 actggagtga atttgaagca gaaggtcgtt tcctcgaagg cacgggcagc atggtcatcg
      661 actatgatca tgaaatgatc tacgcggcaa tatcggagcg taccaattta tcggtcctgg
      721 agaaattcgc tactgccaac ggatttcagg ccattgtatt cctggccact gataaggaag
      781 gccaaccgat ttatcatacc aatgtggtga tggcattggg tgaaaaattt gcagtgcttt
      841 gtgaagaggc gatcgaagaa gaatgggagc tcattgcggt gaaacaatta ctggaatcaa
      901 cagatcatac catcatcccc ataacccgcg accagctcta tgcatttgca ggcaatatgc
      961 tcgagatcaa aaacaaaaaa ggagaatcgc tgatcgtgct cagcgagacg gcaatgagtg
     1021 cactacgtcc ggaccagata caacagctac aaagttttgc aacactgata cccgttgctg
     1081 tccccaccat tgaacaggtg gaaggcggca gtgtacgttg tatgctggcg gagatttttc
     1141 tgcagcgaaa aattaaggaa ggataactac tgctttttct ccttttgctt tcatctgtcc
     1201 gagtggggtg atgaatccac ccagcccatt cttttcaagt ataccgatca gttcgtcttt
     1261 tgcctccggt gaaatggcaa tcagtaagcc gccattagtc tgcgggtccg gcaataactt
     1321 aaaggcttct gccaccgggg tgcctttttc aaattttact ttctcgccgt agctgctcca
     1381 gttgcgggtg gtgccatcgg ggaatacgcc ctgggcgata tactctttca cgccggggat
     1441 agcgggaatc ttatcatacc atacttccgc gctcagtccg ctgccttcgg ccatctcgat
     1501 caggtggccc aggaaaccga agccggtcac atcggtcatg gcatgtacgc cttttatttt
     1561 tcccaactcc tcccctgttt tattcagggt ggtcatttgt ttgatgatta cagccagcag
     1621 ttcgtcggta atcaaccctc ttttctgtgc ggttgacaaa atacctacac caagtggttt
     1681 ggtgagaaag agcaggtcgc cttcttttgc ggtattattt tgtttcaggt tttcaatggg
     1741 aagtatacct gttacggcga ggccaaagat tggttccggt gaatcgatgc tgtgaccacc
     1801 ggctaaagga atgccggctt cgaggcagat gctgcgtccg ccttcgacca ccttctgcgc
     1861 aacatcgggg ggcagtttgt ttaccggcca tcccaatacg gcaatcgcca tcaggggttt
     1921 tcctcccatg gcatagacat cgcttatgga attagcagcg gcgatccggc cgaattcaaa
     1981 ggggtcatcc acaatcggca taaagaaatc cgtggtggaa atgactgcgg tcccattgcc
     2041 gagatcataa acagcggcat cgtcacgact atgattacct accagtaatt tatcattatc
     2101 agggaacgct acagaagttt ttaaaatttc atccagtact ttaggcgaaa ttttacagcc
     2161 acagccggcg ccatgggaga attgggtcaa tcttacagga tcctgcatta tcatttgttt
     2221 attgagaatg caaagttagt cattcagtga aggactgaaa actgtctaag ataagcggag
     2281 tgtatttatt ataactattt gaaacgccgg aacacaatcc ctgaactttt ctggaagtcg
     2341 gagctgaagc gaagattgta aacatcattg ccgtcgcgga tcacgagcag tccggtattg
     2401 ggcgggtatt taccgaggct ttcagcaaac atcacgaggg tgaatgtttc gaggccgtca
     2461 tccatgtgaa tggttttacg gatcgctgag cttttcagac cctggctggg catcactact
     2521 tcgccgttga ggtaaacact cacggtatcg ccatcaattt ccccgttatc atagagggtc
     2581 aggatcaggc tgtcggattt gaaatagacc tcctggttga actctgattt acggccggca
     2641 atcatggttg attttttgat cgggtcttcc acagattttt taaccagcag ggccgttgga
     2701 tcgatgactc tcggtttcgg caggtttgtt tcaaccggat tggcgataac cgctttattg
     2761 ctgaccggtg tcagggccgg ttctgttgct gcaggcttat tgtttacaac ggttgctgtg
     2821 gctggttttg ttttcgccag gttttccgga gcaacagccg cgattttttc ttcaggtgct
     2881 ttagtcgcga ctatcgtttt tggcgcatca gtttgctgat tttttgccga aggatttgtt
     2941 gttttaacaa ctgctttttc ttcctgcgac gggcttggtt taacggttgc agtagttaca
     3001 ggaggtgttg ctggctgtgt ttttaccacc gggctggttg ccggggccgg ttttacggca
     3061 gcgacttcag ggttagcggt actggtgttt gcggtttcgt tagctgaatt agccggggct
     3121 gccgttttaa cgggaggggt ttctgttttt acaggaacaa ccgggatatt tgtttcggtt
     3181 ttggctttgg ccgtcagttt accctgggtc tgaggatccg ggtttttctt ttccggaaca
     3241 gccgtcagcg ccggctcgtt ttttactccc ggtttttcct gggcttggtt attcgttgcg
     3301 accggattgt ttcccggtaa cgcatctgct ggtaaaacgg gtgtcgtttt ggtttccggg
     3361 acagactgta ctgctacttc aggcacgaca ggttgcgccg gttggggcaa ttccggtttt
     3421 ttcaccgaaa gcgatttttc ctcatttttg gccaaaatat cggctttttc ggtgtattct
     3481 gactgaattc gctccggttt tgccactttt acgaatggag caggttcggt tctttctttg
     3541 taaaaagcaa tttcatcggc cttattcagt tcttcgaggt gtggaaatag tttggaagcg
     3601 gtcaggtctt tttcctccgc aaggttcact ttcccggtaa ccgaatagta tttcttagtg
     3661 gcattggtct tccagcttcc atcgagttgc caggtgctat cggtctggtt acgccggaat
     3721 gtgctggtta ctttaatccc cttgtccagt tttcccctga aattataagc aatgatttca
     3781 tcgtctttta cttcacaaat atccccttca atggttcctt tgacacgctt aacaatgtaa
     3841 tagagggtat catttacaat aaattcactg cgggaatagc caaatacctt tccccggtat
     3901 tcggtaaggg cgatttcaaa agactggtcc ttacgtacag agctgctgtc gttgtgaaga
     3961 gcaccaaccc agagaccggt cagggtctga ccataaaaaa acaggggggc gagcaggaac
     4021 aggaaactaa gcca
//
LOCUS       DAOPMW010000310         4021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26487_length_4021_cov_10.465961, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000310 DAOPMW010000000
VERSION     DAOPMW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26487_length_4021_cov_10.465961"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLU11_12520"
     CDS             <1..689
                     /locus_tag="PLU11_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPN59999.1"
                     /translation="YLPLPSGTLSPTQAWLIVSIALSISLGLALFITPYLFGIIALAA
                     AIGWAYSMPPFYLKKHHLTAALSISIVRGLLLNAGGFMVFNYLVNDTLVMPENVKILT
                     VFIMVFSIAISWFKDLPDMEGDAKYNIQTLALLYSPKMVLISGHLLVGMAYLFTIYMK
                     YAEYAVSATPSHQTTILLSGHWLLLGLFIFNAVSIKSTRHFSFKTFYKRFWWFFFAEY
                     LLYLLAYAIN"
     gene            828..1310
                     /locus_tag="PLU11_12525"
     CDS             828..1310
                     /locus_tag="PLU11_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60000.1"
                     /translation="MTNNHLTLTRTVKDDLELFFQFQLDQEAIYLAAFTAKDSHDRAA
                     YFEKHTKFLTDPTIHMQTIHVNDRIVGSIARFYIEGDAEITYWIDRPYWGQGIASSAL
                     SGFLKMEMTRPLFGRVAFDNYGSQKVLEKNGFERIGKDSGFANARQSEIEEFIYRLPA
                     "
     gene            1355..1645
                     /locus_tag="PLU11_12530"
     CDS             1355..1645
                     /locus_tag="PLU11_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase alpha subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPN60001.1"
                     /translation="MEPKKKLKTCKNGHSFYKSSDCPVCPICEEQRKPIDDFLSLLAA
                     PARRALENNGITRLEQLSAYSEQEILKLHGMGKSTIPKLQKLLADKKMSFRV"
     gene            1700..2140
                     /locus_tag="PLU11_12535"
     CDS             1700..2140
                     /locus_tag="PLU11_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60002.1"
                     /translation="MSEVPDPEKKVIMFRLFKKKELPPFPLLQESAYRDIYFVRMTEW
                     DWIDGNRQHIVVTDPHQPRLLTFDPWPELVFIAANGQLTVSEYIHYMARQYSGDVPAL
                     LDQTVLDELKTLLGYRIIEMSPVKCRPDPLFELPRSDRNKYLQK"
     gene            2137..2643
                     /locus_tag="PLU11_12540"
     CDS             2137..2643
                     /locus_tag="PLU11_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60003.1"
                     /translation="MNRLLITLLISGLFTSCTGLTGKNDFYSYTRSGDLWRLPLAEPY
                     ELVSPTNTATDWFLILKPHPVLHPDYLNTGDEFQLSSIDSVGKQKSVIVLHSTNVYWP
                     RLAGSYKSTLILDTVSHKVWLFPDQQGEKIFSRLRELKVTDIQLRPIEPIVRMFQDSL
                     KLPDGWRR"
     gene            2740..>4021
                     /locus_tag="PLU11_12545"
     CDS             2740..>4021
                     /locus_tag="PLU11_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPN60004.1"
                     /translation="MRKINWLLLLLISTGSFAQLSVSRLQTENQSFPFGIDAAQPRFS
                     WQLNSPLRNTMQTAYELKVILNKKAVWQTGKTSSGQSVFISYAGARLESNQQYSWQVR
                     VWDNQGKSSAWSQPAFFQTGLLQPTDWKASWIEAGFIEDSTNRPAQYFRKPFSLNKKI
                     KSATAYITALGMYEAQLNGQRVGDAYLTPGWTSYNKRLQYQVYDVTSLLKAGDNALGV
                     TLGNGWYRGFLAWDDHKNIYGKKPALLMQVMITFTDGSKTIIGSDDSWQTSTGPIRYS
                     EIYHGETYDAREEKTGWASPGYNAAGWVNAKKASVTDRPIVATINEPIRKQERFKPLK
                     IFKTPKGELVADFGQNLVGWVELKVKGNAGDQIVLTHTEVLDKAGNFYTANLRVAKQQ
                     NKYTLKGGAEEFFEPHFTFQGFRYIKVEGFPGELT"
BASE COUNT         1108 a          954 c          912 g         1047 t
ORIGIN      
        1 cttatctgcc tctcccgtcg ggtacattaa gtccgaccca agcctggctg attgtaagca
       61 ttgctttatc aatcagtctt ggattggctt tattcatcac gccctatctt tttggcatta
      121 ttgcattagc tgctgcgatt ggctgggcct actccatgcc ccctttttat ttaaagaaac
      181 atcacctgac cgccgctttg tctatttcca tagtgcgtgg cctcctgctc aacgcggggg
      241 gatttatggt atttaattac ctggtgaatg ataccctggt gatgcctgaa aacgttaaga
      301 ttcttaccgt gttcattatg gtcttcagta ttgctatttc ctggtttaag gatctgccgg
      361 atatggaagg tgatgcgaaa tacaatattc agacacttgc cctcctgtat tcgcccaaaa
      421 tggtactgat cagcggccat ctgctggtag gaatggctta cctgtttacc atttacatga
      481 aatatgctga atatgccgtt tcagcaactc cctcccatca aaccacgata cttctttccg
      541 gacattggct gctgcttgga ctttttatct tcaatgcggt ttccataaaa tcaaccaggc
      601 atttctcttt caaaacattt tacaagcgct tctggtggtt cttttttgcc gaatacctgc
      661 tgtacctgct ggcgtatgcg ataaactaac cggcttgata tgaacctgta aattgtcgat
      721 aagaactggt aatcgccgtc ataatttcca gactacaaat tttaattata cattagtact
      781 gcggaccatt tcccggaaac cctttacgtg aatttgaatt cagatcaatg actaacaacc
      841 acctcacact gacccggact gtaaaagatg atctggaact ttttttccag tttcagcttg
      901 accaggaagc gatttattta gccgctttta cggctaaaga ttcgcatgac agggcggcat
      961 attttgaaaa gcatacaaag tttttaactg atccaacgat ccatatgcag accattcatg
     1021 tgaacgaccg gatcgttggg agtatcgcgc ggttttatat tgaaggggat gccgaaatca
     1081 cttattggat tgaccggcca tactggggac aaggcatagc cagctcggcg ctttccgggt
     1141 ttttgaaaat ggaaatgacc cggccgcttt tcggacgggt ggcttttgat aattatggct
     1201 cacagaaagt gctggaaaaa aatggttttg agcgaatcgg gaaggattca ggatttgcca
     1261 atgcgcggca atcagaaatt gaagaattta tttacaggct gcccgcctga tcattattgg
     1321 cgaacaaaca atttccgcat caactgaata taaaatggag cctaaaaaga agttgaaaac
     1381 ctgtaaaaac ggacattcat tctataaaag ttcggactgc ccggtctgcc ccatttgtga
     1441 agaacaacgc aaaccgatag atgatttcct atccttactt gctgcaccgg ccagaagggc
     1501 ccttgaaaac aatggcatca ccagactgga gcaactatcc gcttatagtg aacaggaaat
     1561 cctgaaattg cacggcatgg ggaagagcac gattcctaaa ttgcaaaaat tattagcgga
     1621 caaaaaaatg tcattccggg tataaatttt cccaaagtga agacagatga atcattttac
     1681 taaaactttt caccggagta tgtctgaagt gcctgatcct gaaaaaaaag taatcatgtt
     1741 ccgtcttttt aaaaagaaag aattgccgcc tttccccctg ttacaggaat ctgcttacag
     1801 ggatatatac tttgtacgta tgactgagtg ggactggatt gacggcaaca ggcagcatat
     1861 tgtggtaacg gatccgcatc agccgaggtt attgacgttt gacccctggc ctgaactggt
     1921 gtttatcgcg gccaacggac agctgaccgt cagcgaatat attcattata tggccaggca
     1981 atattccggt gatgtgcctg cgctgctgga ccagaccgtg ctggatgaac tcaaaaccct
     2041 gctgggatac cggattattg agatgagtcc ggtcaagtgc cggccagatc cgctttttga
     2101 acttccccga tctgatcgaa acaaatatct tcaaaaatga accgcctgtt aatcacctta
     2161 ctgatttccg ggcttttcac cagttgcacc ggcctgactg gcaaaaatga tttttactcc
     2221 tacacccgaa gcggtgacct ttggcgtttg cctttagccg aaccctatga actggtctcc
     2281 cccacaaata cagccaccga ctggttcctg atcctgaagc ctcatccggt ccttcatccg
     2341 gattatttga ataccggtga cgagtttcaa ctcagttcca ttgacagcgt tggaaaacag
     2401 aagtcagtga ttgttttaca tagtacgaat gtatactggc cacgtttagc cggctcgtat
     2461 aagagtaccc tgatcctgga taccgtcagt cataaagtct ggttattccc cgatcagcag
     2521 ggagaaaaaa tcttcagccg gctgcgtgaa cttaaagtga cagatatcca gttacggccg
     2581 attgaaccaa tagtcagaat gtttcaggac agtctgaaac taccggatgg gtggaggcga
     2641 taaaaaggat gcttaatcgg catagcacat gacagcccct gcctctcccg gcagtatatt
     2701 taattcggta tcttactgtt ttaaacgaat gctctcccca tgagaaaaat caactggctg
     2761 ctcctgctgc tgatcagcac cggcagtttt gcccaactca gtgtttccag gctccagacc
     2821 gaaaatcagt ccttcccctt cggcatcgat gcggcccaac cgcgtttcag ctggcagctg
     2881 aacagccccc tgcgcaatac gatgcaaaca gcttatgaac tgaaagtgat actgaataaa
     2941 aaagcagtct ggcaaacggg caaaaccagc agcggccaat ccgtttttat ttcttacgcg
     3001 ggagccagac tggaaagcaa tcaacaatac agctggcagg tacgggtatg ggataaccag
     3061 ggtaaaagca gtgcctggag ccagccggca ttttttcaaa ccggtttact gcaacctaca
     3121 gactggaaag ccagctggat cgaagccggt tttatcgaag acagtaccaa tcgtccggcc
     3181 cagtacttcc gcaaaccatt ttccctgaat aaaaaaataa aatccgccac ggcctatatc
     3241 accgcattgg gcatgtatga agcgcagcta aacggtcaac gtgtcggtga tgcttacctg
     3301 acacccggct ggaccagtta taataaacgg ttgcaatacc aggtttatga tgtaaccagt
     3361 ttactgaaag ccggtgacaa tgcactgggt gtaacgctgg gcaatggctg gtaccgcggc
     3421 ttcctggcct gggatgatca taaaaatatt tatggaaaga aaccagccct gctgatgcag
     3481 gtgatgatca cgtttaccga cggcagtaaa accattattg gcagtgatga cagctggcaa
     3541 accagtaccg ggccgatccg ttactccgaa atctatcatg gggagaccta tgatgcccgg
     3601 gaagaaaaga ccggctgggc cagtcccgga tacaatgctg cgggttgggt gaatgcaaag
     3661 aaagcttcgg tgactgaccg gcccatcgtt gccaccatca atgaacccat ccgtaaacag
     3721 gaacgcttta aaccccttaa aatattcaaa actcccaagg gtgaactggt ggccgatttc
     3781 ggtcaaaacc tggtgggctg ggtagaactt aaagtaaaag gtaatgccgg agatcagatc
     3841 gtactcacgc atacggaagt attagacaaa gctggtaatt tttataccgc taacctgcgg
     3901 gttgccaaac aacaaaacaa gtataccctc aaaggcggtg cggaagaatt ctttgaaccc
     3961 catttcactt tccagggttt ccgctatatc aaagtggagg gatttccagg tgaactgact
     4021 g
//
LOCUS       DAOPMW010000311         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26561_length_4013_cov_6.492926, whole genome shotgun sequence.
ACCESSION   DAOPMW010000311 DAOPMW010000000
VERSION     DAOPMW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26561_length_4013_cov_6.492926"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            608..1147
                     /locus_tag="PLU11_12550"
     CDS             608..1147
                     /locus_tag="PLU11_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60005.1"
                     /translation="MKNMLSIRLIRLILAFSVVSFLGTTCDDNEEPVLDYVGKWVAEK
                     QVAVATGYVTIKYYLELTNKGFKETFIRPPRHYHSRGDQLTLEGIVYATENTVKLVIH
                     KLSVSKYDVSTDTASAPHEIHTYKDQDFGFNFEGMVYSISNHQSEYSFIDGKLIFKTD
                     FNMDGIYSEQEKSVYTKQL"
     gene            complement(1618..2472)
                     /locus_tag="PLU11_12555"
     CDS             complement(1618..2472)
                     /locus_tag="PLU11_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPN60006.1"
                     /translation="MMSGALLASENPRDTVVYLGTNGRLVPEEGALFKKEVVTTKMNK
                     KEEISWKKVNGRWEKTATLKIRVLKGDTWELRQGAGRKEGVLLRQYLPAGEGKWQFLE
                     QVGGKLIRKGFTLSRFPLLLDGEVTDYYPNGKTRSVAQYRRNELEGNFNWLENGSKYI
                     DSVFYSVDEEPMLTGGNLFLHQYMKNALEKSGLDLTVLTGNMVLGFVVMETGDLAGVR
                     VIEGFNPRLNEVAVAALQNLSLQWNPAHLDGRPVRYLQLFPINFIYKEANFQSLDFDG
                     WMIHYNKY"
     gene            2903..3622
                     /gene="mtgA"
                     /locus_tag="PLU11_12560"
     CDS             2903..3622
                     /gene="mtgA"
                     /locus_tag="PLU11_12560"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPN60007.1"
                     /translation="MVRRIFRFLLKAAAWFIGLTVLLVLFFKWFPVPVTPLMVIRTVQ
                     QATRGKEIRMQHDWVAMKRISPNLQLAVIASEDQNFVYHRGFDLEAIDKALKDSQKRN
                     RKPRGASTISQQTAKNLFLWPGRSWVRKGLEVWFTGWIELLWSKKRILQVYLNIVEMG
                     NGIYGAEAAAHHFFGVPAEKLTSRQAATLAAVLPNPRRYNAASPGPYVSGRIGWIQGQ
                     MQQYGPLDLKPPRKHKKQKKK"
     gene            3737..>4013
                     /locus_tag="PLU11_12565"
     CDS             3737..>4013
                     /locus_tag="PLU11_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60008.1"
                     /translation="MGKKGEAPLYTENRAPLRANPFIELPLGAIRPEGWLREMLLRQK
                     TGATGHLDELYPAVMGPRNGWLGGDGDQWERGPYWIDGLLPLAWILED"
BASE COUNT         1111 a          944 c          902 g         1056 t
ORIGIN      
        1 ggaaggaatg caccaattat ggaattggct tttaggtatt tacaaaattt ttgtcaaaaa
       61 aaaagctata tgtgccctgg ttgacagcat ctccaaaggt tgtatttcgt accttcgaat
      121 aactatttcc ggtaatatga gcctgggttg ttccgggttt ataaaaccgg aattgatccc
      181 gacagtttaa gcccgattca aattcgaagg catggaagaa aaaccagggg ttggtctaaa
      241 tacagtttga cttatttcgc taatatgtta ttggcaataa tatagatcaa ttttaagggt
      301 gttgtaaatt tttatcatgt acttagtaaa attttataaa tcgctagaca agaatgttct
      361 ctctgaaatc tatgatatta gagatacact tacaattcaa tttcgaggag aggatgaagc
      421 aaggcaattt aacagacact tttctttcaa tcttaactat gatggcaaat atgtttcctt
      481 ttgcaaggag ttaagttcaa agagtaaaat tttcaagaac tactatgagt caatccaggt
      541 tggaggagat atttctcctt caaattatgc aatgatactc cacgactaca attaaataga
      601 tttcaaaatg aaaaatatgt tatctataag attaatccgt ttgatcttag ctttttcagt
      661 agtgtctttt ttgggtacaa cctgtgatga taacgaagaa ccagtcctgg attatgttgg
      721 gaaatgggta gcggaaaaac aagttgcagt ggcaaccgga tatgtaacaa tcaaatacta
      781 cctcgaacta acaaataaag gatttaagga aacttttatc cggcctccac gacactatca
      841 ttctaggggt gaccagctaa ctttggaagg aatagtttat gctactgaaa atactgtaaa
      901 actggttatt cataagttaa gtgtgtcaaa atacgatgtc tccacggata cagcttcagc
      961 acctcacgaa atacatactt ataaagatca ggatttcggg tttaatttcg aaggcatggt
     1021 atattcaatc agtaatcatc aatctgagta tagctttatt gacggaaaat taatatttaa
     1081 aactgatttt aatatggatg gtatatattc tgaacaagaa aaatcagtat ataccaaaca
     1141 gctatgactt cacctgatta taaaaggtaa accacacaag agaataattg accgggttgt
     1201 aaaacaagct tcccttttta tcacgtttaa ttctctgtta caaaaccatc ccttacagta
     1261 atggaatagt taaatccgtt ggattaagga ttagcgaact ataaacaaga tgctgggatt
     1321 ttaaaactcg ctggggatcg tatcaacaga aaggtatgtt tgcggaagtc ctgtgagcca
     1381 ccacctacaa ccggcgcatc aacctttaca tcaacgtttg ggaggtctgc aatgacatta
     1441 cctgtatgag aaggatgaag gactctggct catctccacc agttggtaat acccacattc
     1501 acacgaatac tgcggattct cgtttcgcag ttccgtgtcc ttcgggcatc ccctactttc
     1561 acctaagcct tgacctggcc ggaacgaagt gaaggcctgc tgacctcaac ctaaacctta
     1621 gtatttattg tagtggatca tccagccgtc gaagtcgagc gactggaagt tggcttcctt
     1681 atagatgaag ttgatgggga aaagctgcaa ataacgaacc ggcctgccgt cgaggtgcgc
     1741 cgggttccat tggagactca ggttttgcag ggcagcgacg gccacctcat tcagccgggg
     1801 attaaaccct tctatgaccc tgaccccggc aaggtcgccc gtttccatca ccacgaagcc
     1861 cagcaccatg tttcccgtca agacggtcaa atcgagcccc gacttctcca aggcattttt
     1921 catatactgg tgcaaaaaga gatttccgcc cgttagcata ggttcctcat ccacactgta
     1981 aaataccgaa tcaatatact tgcttccgtt ttccagccaa ttaaagttgc cctcgagttc
     2041 attccggcgg tattgcgcca ccgaccgggt tttgccattg ggataatagt cggtcacctc
     2101 gccgtcgagg agcagaggaa accggctcag ggtaaacccc tttcgaatca gtttaccccc
     2161 gacttgttcc agaaactgcc atttcccctc cccggcagga agatactgcc gcaacagaac
     2221 cccctcttta cgcccggcac cctggcggag ctcccaggta tcccccttca atacgcggat
     2281 tttcagagta gcagtttttt cccaccgccc attgaccttc ttccaggata tctcttcctt
     2341 cttgttcatc ttcgtggtga ccacctcctt tttgaacagg gccccttcct caggaaccag
     2401 cctgccgtta gtccccaggt aaaccaccgt atcccgcgga ttttctgagg cgaggagtgc
     2461 accggacatc atgacaagaa aaagagataa caacgcaggg aataagcccc cccttgaaaa
     2521 agacaaattt aagaggttgc ttgctgagtt tgaatgggaa tcacaatgaa acatatgaaa
     2581 atgatatttt aagctcttgt tttccatgta cctatagctg acttgatgac tacttattgt
     2641 ctgaaaagaa ttgcatacct gttgcatgac tggtatgaca tgataatggg acaaatattt
     2701 atccctgcta aattatataa ttcctgcctt attccgttac gtaaatggcc cttcccgtac
     2761 cggaattcaa gtaaagggat tctgaaagga caagatgatg ctgctccggc tggccggttt
     2821 actacctccg gggttatgtt tggaatccgt cgaaatatac tatttttaag ggcatcaaaa
     2881 gaacgaattg cgtatagcag atatggtccg acgtatattt cggtttttgc tgaaagctgc
     2941 cgcctggttc atcgggctga cggtcctcct tgtgcttttt ttcaaatggt tcccggtgcc
     3001 ggttactccg ctgatggtaa tccggacggt ccagcaggcc accaggggca aagagatccg
     3061 catgcagcac gactgggtgg ccatgaaaag gatctcgcca aacctccaac tggcagtcat
     3121 cgcttccgaa gaccagaact tcgtttacca ccggggattt gacctggagg ccatcgataa
     3181 agccctgaaa gattcacaga agagaaaccg caaaccccgg ggggccagca ccatctccca
     3241 gcaaacagcc aagaatctat ttctctggcc cgggcgaagc tgggtccgca agggactgga
     3301 agtgtggttc acgggttgga tagaattgct ctggagcaaa aagcgtattc tacaggtcta
     3361 tttgaacatc gttgagatgg gtaacggcat ttacggggcg gaagcggccg cccatcattt
     3421 tttcggtgtt cccgcggaaa agctcacctc ccgtcaggcc gccaccctgg ccgccgtcct
     3481 gcccaatccc cgacgttaca acgccgcttc accgggccct tatgtttcgg gacggatcgg
     3541 ttggatccag ggacagatgc aacaatatgg tcccctggac ctgaaacctc cccggaaaca
     3601 caaaaaacag aaaaagaaat aaccattttc aagagggtag ttcccggtgg cctgaccatt
     3661 tatatcaagc cattatatgc acattatgaa agcaactgca atgatcttat ttttcgccct
     3721 tctcgcgggg tgcggcatgg gaaagaaagg ggaggctccc ctttatacgg agaacagggc
     3781 acccctgcgt gccaatcctt ttattgagct tccgctgggt gccattcgtc cggaggggtg
     3841 gctgcgggag atgcttctgc gccaaaaaac aggagccacg ggtcaccttg atgaactcta
     3901 tcctgcggtg atgggacccc gcaacggatg gctcggaggc gacggcgacc agtgggaacg
     3961 gggtccctac tggatcgacg gtctccttcc cctggcctgg atcctcgaag acg
//
LOCUS       DAOPMW010000312         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26563_length_4013_cov_5.322890, whole genome shotgun sequence.
ACCESSION   DAOPMW010000312 DAOPMW010000000
VERSION     DAOPMW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26563_length_4013_cov_5.322890"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLU11_12570"
     CDS             complement(<1..502)
                     /locus_tag="PLU11_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPN60009.1"
                     /translation="MVDLSSYDPNSVSNPNNNIFGLPSDEESARLIILPVPWEVTVSY
                     GAGTARAPEAILKASYQVDLFDPEMPDAWKQGFYMRPVDRKLLMKSDYLRKEAELYID
                     YISKGDDVNANQFMCKTLKEVNEGGVYLTEWMHAQSKALLDQDKLVGVLGGDHSVPLG
                     YMKALGE"
     gene            complement(898..1962)
                     /locus_tag="PLU11_12575"
     CDS             complement(898..1962)
                     /locus_tag="PLU11_12575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPN60010.1"
                     /translation="MNFPKFSGSAQTFHAELKKRINDYFNEAGKSYTGNFQLYFKAVV
                     LVIAYVAVYTHLVFFTPATWLALTECVVLGGLTAAIGFNIMHDGMHGSFSRKKWVNYV
                     AGLSLNFLGANNFMWKTKHNIIHHTYTNIEGLDDDIEARPLLRLCDTQKYYKIHRFQH
                     WYFWAAYSLLYIWWVFVTDYKKYFRKKIGEVPLKPMSAWDHISFWGFKVLNIFIFVVI
                     PITQLGFTSWLIGYLVFGLFAGFVLSIVFQLAHTVEHTHFPHADDHNRMEDEWAIHQL
                     KTTANFATNNKVISWLVGGLNFQVEHHLFPKISHVHYPAISKIIKKACEDFGVTYIEY
                     PKMRLALASHVHHLKNLSRK"
     gene            complement(2075..2812)
                     /gene="bshB1"
                     /locus_tag="PLU11_12580"
     CDS             complement(2075..2812)
                     /gene="bshB1"
                     /locus_tag="PLU11_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HPN60011.1"
                     /translation="MKVDILAIGVHPDDVELGCSGTLINEVKSGKKVAILDLTQGELG
                     TRGTVETRYEEAANAAMIMGVHARENLKMRDGFFRNDEEHQLQLIAAIRKYQPSIVIG
                     NILKDRHPDHGRAGNLITESCFLSGLAKIETKDSEGTPQAKWRPTYVLQYMQDWYHEP
                     DFLVDISDVFAQRMKSIEAYTTQFHNGNAPQEGPQTYISKPGFLEAVVARARMFGKRI
                     GVDYAEGFLSEKKLGLRDLGALIQNET"
     gene            complement(2884..3594)
                     /locus_tag="PLU11_12585"
     CDS             complement(2884..3594)
                     /locus_tag="PLU11_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPN60012.1"
                     /translation="MRARFFHTIALPALLVLMLTIGTTVNAQVVLPEPAVDSTDWALE
                     YMKEQRKKWGPNDTIILPAIWYQSEIVNYKEMEMAWVSNLSPEKLQKYIQEWTRLRNA
                     VYVTYPYARIAGGTINEINATLTGITSKKERKSVIKSREKELKSEFTEPLSNLSVYQG
                     KILMKLINRQTGNNCYEIIKEYKGGFNARFYQTIAFFVGGNLKQQWNLKENKMDRDIE
                     NIVREIDGSWYGNQYRAR"
     gene            complement(3672..>4013)
                     /locus_tag="PLU11_12590"
     CDS             complement(3672..>4013)
                     /locus_tag="PLU11_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HPN60013.1"
                     /translation="YLTELPLVVHAKTGSNYTLTSRLANAQNGIFAIPDTLHAEGLII
                     QLQQVNPDKSVEVGVKESDALLKYVTLKAYRFPFILLLWAGVYITAMGILISMVRRIQ
                     LNRKKKAGEEI"
BASE COUNT         1040 a          913 c          905 g         1155 t
ORIGIN      
        1 tttctcccag cgctttcata tagcccaggg gtacgctatg atcacctccg agtacaccca
       61 ccagtttatc ctgatccagc agggccttgc tctgggcatg catccattca gtaagataaa
      121 cgcccccttc atttacctct ttcagggttt tgcacatgaa ctggttggca ttcacgtcat
      181 ccccctttga aatatagtcg atatataatt ccgcttcctt acgcaggtaa tcgctcttca
      241 tgagaagttt acgatccacc ggacgcatat agaaaccctg tttccaggca tccggcattt
      301 cgggatcaaa cagatcaacc tggtaactgg ctttgagaat ggcctcaggt gcacgggcgg
      361 tacccgcacc ataacttacc gttacctccc agggtacggg cagaataatc agccgggcac
      421 tctcttcatc actgggaagt ccgaaaatgt tgttattagg attgctgacg ctgttagggt
      481 cgtaggagga aaggtctacc attttgattt catattgaaa aacggtgcaa gttacaggtg
      541 ttttctggta gctgcaaaaa gaatccttca tccctttttt ttcccgcaaa gcaaagaaga
      601 cagatttaaa gaggcagatt tactcctggt ctggaagaat gggtctttcg atctggataa
      661 gatatttctg gtatccgatt gtcttttaat tctttgcggg acaaaaaaat ccagcaactg
      721 tgggattcgc ccgcaaagca aagaagatag tcttaaagaa attgtttgaa ctacaggtca
      781 ggaactgtaa acatttcaat tgtaatctac aaaccgggta tgatttttct acaatcgggt
      841 tgtcttttaa ttctttgcgg gacaaaaaaa atcccgcaac ctgcgggatt cagtatttta
      901 ttttctgctg agattcttca ggtggtgcac atgcgaagca agggccagcc gcattttggg
      961 gtattcgata tacgttacac caaagtcctc acaggctttc tttatgattt tgctgatagc
     1021 aggataatgc acgtgtgaga tcttagggaa caggtggtgt tcaacctgaa aattcaaacc
     1081 gcccaccagc caggaaatca ctttattatt agtggcaaaa ttggctgtcg tttttaactg
     1141 gtgaatcgcc cattcatctt ccatccggtt atgatcatcc gcatggggga aatgggtatg
     1201 ctcaacggta tgggccagct ggaaaacaat actcaggaca aagccggcaa ataacccaaa
     1261 tacaaggtag ccgatcagcc aggaagtaaa gccaagttgt gtaataggga tcactacgaa
     1321 aataaaaata ttcaacactt taaatcccca gaaagagata tgatcccagg cactcatcgg
     1381 tttcagcggt acttcgccga tctttttacg gaaatacttt ttatagtcgg ttacaaaaac
     1441 ccaccagata tagagcaaag aatacgcagc ccagaaatac cagtgctgga agcggtgaat
     1501 cttataatat ttctgtgtgt cacaaagacg gagcaggggg cgcgcttcga tatcatcatc
     1561 cagtccttcg atatttgtat acgtatggtg gatgatattg tgcttggtct tccacatgaa
     1621 gttattggct ccaaggaagt tcagcgaaag tccggccaca taattgaccc attttttccg
     1681 gctgaaacta ccatgcatgc catcatgcat gatgttgaaa ccaatggctg cagttagtcc
     1741 gcccagcacc acacattctg tgagtgccag ccaggtggcc ggtgtgaaaa acaccagatg
     1801 ggtatatact gccacatagg cgattaccag cactacggct ttaaaataga gttggaagtt
     1861 accggtgtag gatttgccgg cttcgttaaa atagtcgttg attcttttct tgagttctgc
     1921 gtgaaaagtc tgtgcgctac ctgaaaattt tggaaaattc attattaatg cttgtttcga
     1981 taggtaaagg taacccttaa agcccatatt tcgttaaaaa ctatccacta catgaggaga
     2041 atcatttcta gttaaagtat tgcaaatttg tgacttatgt ttcgttttgg atgagcgcac
     2101 cgaggtcacg gagccccagt tttttctcac tcagaaagcc ttctgcataa tccactccaa
     2161 tccgttttcc aaacatccgt gcccgggcta ccacggcttc gaggaagcct ggtttagaga
     2221 tataagtttg cggaccttcc tgcggggcat taccgttgtg aaactgggtg gtataagctt
     2281 caattgattt catccgttgt gcaaaaacgt cactgatatc taccaggaaa tccggctcat
     2341 ggtaccagtc ctgcatatat tgaagcacat aggtggggcg ccattttgcc tgtggggtgc
     2401 cttcgctgtc ttttgtttcg atttttgcca gtccggaaag aaaacaactt tctgtaatca
     2461 ggttgccggc ccgcccatgg tccgggtgac ggtccttcag gatatttccg atcacaatgg
     2521 aaggctggta tttacggatg gcggctataa gctgcagttg gtgttcttca tcattgcgga
     2581 aaaagccatc ccgcattttc aggttttcac gggcatgtac acccatgatc atggcggcat
     2641 tggcggcttc ttcgtacctg gtttcgacgg tacctcttgt gccgagttcc ccctgggtaa
     2701 gatccaggat cgctactttt tttccagact taacttcatt gatcagggtg cctgaacagc
     2761 ccagttccac atcatccgga tgaacgccaa tggccagaat atccactttc atacttatgg
     2821 ttggttttaa acccggcaaa gatatttgtt tcggaggaga gtcgcttttt tagaaaggcg
     2881 ggcttaacgg gctctgtatt ggttgccata ccagctgcca tcaatttcac ggacaatatt
     2941 ttctatatcc cggtccattt tattttcctt cagattccat tgctgtttca ggttacctcc
     3001 cacaaagaag gcaatcgtct ggtaaaaccg ggcattaaaa ccacctttat attctttgat
     3061 gatttcataa caattattcc ccgtctgccg gttaataagt ttcatgagga ttttgccctg
     3121 gtagaccgaa aggttggaca acggttctgt gaactcggac ttcaattcct tttcccgtga
     3181 tttgataacg gattttcttt ctttttttga cgtgatccca gtcagcgttg cattgatttc
     3241 attaatagtg ccgccggcaa tccgggcgta gggatacgtg acataaaccg cattgcggag
     3301 ccgggtccat tcctggatat acttctgtaa tttttccggg gagaggttag acacccaggc
     3361 catttccatt tccttataat tcacaatttc cgactggtac cagatggcgg gaaggatgat
     3421 ggtatcattc ggcccccatt tcttccgttg ttctttcatg tattccagtg cccaatcggt
     3481 ggaatcaaca gccggttcag gcaataccac ctgtgcgttg acagtggtac caattgtcag
     3541 catcaaaact aacagggctg gtaaggcgat ggtatggaag aatcgggcac gcatgcttct
     3601 ttaaagttag aaaacagaaa cgatatttcc gaaaagacag taaaaccagg cccggcgctg
     3661 tttcaataat cttaaatttc ttccccggcc ttctttttcc ggttaagttg aatccggcgg
     3721 accatcgaga tcaatatgcc catagccgta atataaacac ctgcccacaa taataatatg
     3781 aatgggaacc ggtaggcttt gagtgtcacg tatttaagca aagcatctga ttcttttact
     3841 ccaacttcaa cggatttatc cggatttacc tgttgtaact gtatgatcag gccttctgca
     3901 tgaagggtgt caggaatggc aaaaatgccg ttttgtgcat tcgccagccg gctggtcagc
     3961 gtgtaatttg accctgtttt ggcatgaacc accaggggga gttcggtcag gta
//
LOCUS       DAOPMW010000313         4011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26579_length_4011_cov_7.544237, whole genome shotgun sequence.
ACCESSION   DAOPMW010000313 DAOPMW010000000
VERSION     DAOPMW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26579_length_4011_cov_7.544237"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLU11_12595"
     CDS             <1..599
                     /locus_tag="PLU11_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPN60014.1"
                     /translation="WNEGTEQEIYTKEELIQKFSMERVHSAGAKFDYEKAKWFNHEWL
                     KRLEVGSLKLEVKTLLTEKGITVTDDSVLEKVIGLVKDRCQLLTDFVQQSDYFFKAPE
                     TIDTAAVKPKWNEAKQLFFAELIRNYELATDWEAHTLENNLKEIAAVNDLKPGELMLP
                     LRIMLVGGKFGPGVFDIAAILGKAETIKRMETALALLQ"
     gene            complement(616..1605)
                     /locus_tag="PLU11_12600"
     CDS             complement(616..1605)
                     /locus_tag="PLU11_12600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN60015.1"
                     /translation="MQYRKFGNTDLRVSEIGFGAWAIGGGAMIGSTAIGWGDVDDRVS
                     EAAILASVEAGINFFDTADIYGLGHSEELLGRTIGQMKEILIASKVGNVSRNEQFTVD
                     YSAEYITTACDQSLKRLKRDRIDFYQLHTARLTHLQQGDCIDAMLQLQQAGKIRFWGL
                     SLNTFDPFPEAGYLMEHEFGNGFQLVLNLINQHSLPLLKVAGEKGYGIIARMPLQFGL
                     LTGKFDGGASFPENDHRRKRLVPSIVEACAQALAPVWQLCEKYNCSKTQLALSYLLSY
                     PEVSVIIPGIRTPEQVQGNTTGLFQLDVQDLKLIESLGSSTFVSLMEQIQKQG"
     gene            1666..2073
                     /locus_tag="PLU11_12605"
     CDS             1666..2073
                     /locus_tag="PLU11_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPN60016.1"
                     /translation="MNRPIRVLVAKCGLDGHDRGAKVIATALRDAGMEVIYTGLRQTP
                     EMVVNAALQEDVDAIGISILSGAHNTVFPKVITLMKEKQLDDVLLTGGGIIPDEDIEE
                     LNKIGVGRLFHPGTNTHEIADYITGWVKEHRNF"
     gene            2271..3716
                     /locus_tag="PLU11_12610"
     CDS             2271..3716
                     /locus_tag="PLU11_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPN60017.1"
                     /translation="MESHSLLASYGTDPRVWDEMFGKNGIRSAYQNIVSAIQDLSADE
                     MTHKDELAKKLFMSQGITFTVYSSGEGIEKIFPFDIIPRILEAAEWKHIEEGIKQRLK
                     ALNIFLKDIYHQQFIIKDGIVPAKLIYSCPNFVRQMVNVNVPFDIYTHISGIDLIRDH
                     DGTYYVLEDNLRTPSGVSYMLENRSITYRIFPDLLPRNKVRSVKDYPDLLLKNLVTLA
                     NRQKSDPTVVLLTPGIYNSAYFEHTTLARMMGIELVEGSDLLVDNHKVYMKTTSGLKQ
                     VDVIYRRVDDDFIDPLVFRGDSMLGVPGIYGAYRTGNVAIVNAMGNGVADDKAVYSYV
                     PAMIRYYLNEEPILKNVPTYQLELPENRKLVFENMDKMVIKKTNESGGYGMLIGSAAT
                     EKQMEEFRVAVEDDPRSYIAQPIISLSSAPCYINGILQPRRVDLRPFALYGPDGIDIV
                     PGGLTRVALKEGSLVVNSSQGGGSKDTWVLE"
BASE COUNT         1059 a          950 c          974 g         1028 t
ORIGIN      
        1 gctggaatga aggcaccgaa caggagatct ataccaaaga agagctgatc caaaagttct
       61 ccatggaacg ggtacacagt gccggggcga aatttgatta tgagaaagcg aagtggttta
      121 atcatgaatg gctgaagcgg ttggaggttg gaagtttgaa gttggaggtt aagaccctgc
      181 tgactgaaaa aggcattact gtaaccgacg attcggtatt ggaaaaagta attggtttgg
      241 tgaaagaccg ttgtcagttg ctcaccgatt ttgtacaaca gtccgactat ttctttaaag
      301 caccggaaac tatcgataca gcagcggtga aaccgaagtg gaatgaagcc aaacaattat
      361 tctttgcgga actgatccgg aattatgagc tggctactga ctgggaggcc catacgctgg
      421 aaaataattt aaaggagatc gctgcagtaa atgacctcaa gccaggtgaa ctgatgcttc
      481 ccctccggat tatgctggtg ggtggtaaat tcggtccggg ggtttttgat attgccgcta
      541 tactgggaaa agcagaaacc atcaaacgga tggagacggc tctggccttg ttgcaataga
      601 tcattccgcc gcaaatcaac cctgcttttg tatttgctcc atcagcgaaa caaaagtact
      661 gctgcccaaa gattcaatca gtttgaggtc ttgcacatcc agctgaaaca atcccgttgt
      721 attgccctgt acctgttccg gcgtgcggat acccggaatg atcacggaca cttccggata
      781 actgagcagg tagcttaaag ccagctgcgt tttgctgcaa ttatattttt cacagagctg
      841 ccagaccggg gcaagcgcct gcgcacaagc ttccacgatt gagggtacca gccgtttacg
      901 tcggtgatca ttttcaggaa atgaagcccc tccatcaaat ttacctgtga gcagaccaaa
      961 ctgcaaaggc atgcgggcaa tgatcccgta tcctttttcg cccgccactt tcagcagcgg
     1021 aagactgtgc tggttgatca ggttcagtac cagctgaaac ccattcccga attcatgttc
     1081 catcaaatac cccgcttccg gaaaaggatc aaatgtatta agggagagtc cccagaagcg
     1141 gatctttccg gcctgttgca attggagcat ggcatcaata caatctccct gttgtaaatg
     1201 ggtcaaacgg gcagtatgta attgataaaa gtcaatgcgg tctcttttga gccttttcag
     1261 ggattggtca caagctgttg tgatatactc agcagaataa tccacggtga attgttcatt
     1321 ccgcgacaca ttccccactt tggaggcaat caggatctct ttcatctgcc cgattgttct
     1381 acccagtaat tcctctgaat ggcccagtcc ataaatatca gcggtatcaa agaagttaat
     1441 accggcctca acggatgcca ggatagctgc ttccgacaca cgatcatcca catcccccca
     1501 tccgatggcc gtggagccaa tcatggctcc gccgccaatg gcccaggctc caaagccaat
     1561 ttcactgacc cgcaggtccg tatttccgaa tttccggtat tgcatgctgt aatttacagg
     1621 gtagtgaagg aaagcaccta attttgtatc ctgaaatttt gccagatgaa cagacctatc
     1681 agagtattag tggccaaatg tggactcgat ggccatgacc gcggtgccaa agtaattgcc
     1741 accgccctgc gggatgcagg aatggaagta atttataccg gacttcgtca gacccccgag
     1801 atggtggtga acgccgccct gcaggaagat gtggatgcca ttggtatcag cattctgtcc
     1861 ggtgcacaca ataccgtttt cccgaaagtg attaccctga tgaaagaaaa acaactggat
     1921 gatgtattac tgaccggcgg tggaattatt ccggatgaag acattgagga gcttaataaa
     1981 atcggcgtgg gccgcctttt ccatccgggt accaataccc atgaaattgc ggactatatt
     2041 actggttggg taaaagaaca ccgtaatttc taactgttac atccccgttt tactgaccgg
     2101 gtaacccctg gcctggtttt gattttcacc ccccaaaggg tccacggaat aattgtttaa
     2161 ttcaattatc catcttatct tccggctgcg cagtgattac tactgttgcc cgctacggtt
     2221 acatccactt agactaccac tacttctttt ttatcgtaaa ctgattacgt atggaatctc
     2281 attcccttct ggcgagctat ggtacagacc cccgtgtctg ggatgaaatg ttcggcaaaa
     2341 acggcatccg ttccgcctac caaaacattg tgtccgccat ccaggatttg tcagccgatg
     2401 aaatgaccca taaggatgaa ctggccaaaa aattatttat gagccagggc atcaccttta
     2461 cggtttacag cagcggcgaa gggattgaaa aaattttccc cttcgacatc attccccgta
     2521 tcctggaagc agccgagtgg aaacatattg aagaaggcat caaacagcgg ctgaaagcgc
     2581 tgaatatttt cctgaaagat atttatcacc agcagttcat cattaaagac gggatcgtac
     2641 cggccaaact gatctactcc tgtcccaact tcgtgcggca gatggtgaat gtgaatgtgc
     2701 cctttgatat ctatacccat atttccggca tcgatctgat ccgggatcat gacggcactt
     2761 attatgtact ggaagataac ctgcgtaccc cctctggcgt ttcttatatg ctggaaaacc
     2821 gcagtatcac ttaccgaatt tttccggatc tgttgcctcg taataaggtt cgttccgtaa
     2881 aagattatcc ggatctttta ttgaaaaatc tggttaccct ggccaatcgc cagaaaagtg
     2941 accccaccgt ggtattgctc actcccggta tttataactc tgcctatttt gaacatacca
     3001 ccctggcccg gatgatgggc attgagctgg tggaaggcag tgacctcctc gtggataatc
     3061 ataaagtgta catgaaaacc accagcggac tgaagcaggt ggacgtgatc tatcgccgcg
     3121 tggatgatga ttttattgat ccgttggttt tccggggcga cagtatgctt ggtgtacccg
     3181 gcatttatgg cgcttaccgt accggcaatg tggccattgt aaatgccatg ggcaacggag
     3241 tggccgatga taaggccgtg tactcttatg tacctgccat gatccgttac tacctcaatg
     3301 aagagccgat cctgaaaaat gtacccacct atcagctgga acttcctgaa aaccggaaac
     3361 tggtttttga gaatatggat aaaatggtga tcaagaaaac caatgagtcc ggcggctatg
     3421 ggatgctgat cggcagcgcc gccacggaga aacaaatgga agaattcaga gtggcggtgg
     3481 aggatgatcc gcgcagttat atcgcgcagc cgatcatcag tctttcctcc gcgccctgtt
     3541 atatcaatgg catcctgcag ccaagacggg tagacctgcg tccctttgcc ttgtacggac
     3601 cggacggcat tgatattgta ccaggcggac tgaccagggt ggcgttgaag gaggggtcgt
     3661 tggtggttaa ctcttcacaa ggtggaggca gcaaagatac ttgggtactt gagtaatggc
     3721 aagctacgag ctacgagtta cgagccaaaa gtccgaagta tcaggctcgc cattcgtaac
     3781 tcgccactcg caaccctgta ctcttgaatt aaaacagatt ttacaaattt ttagagagat
     3841 tacttatgct tagtcgtgtc gccgattccg ttttctggat ggcccgttat atggaacgga
     3901 ccaatgggat gttgcggata ttacgaacca attatatttc ctcgcaggat gaagtaaagg
     3961 atttcaactg gcataccctg ttgcgtaatt acagtgatgc cggggcggag g
//
LOCUS       DAOPMW010000314         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26689_length_4001_cov_5.361885, whole genome shotgun sequence.
ACCESSION   DAOPMW010000314 DAOPMW010000000
VERSION     DAOPMW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26689_length_4001_cov_5.361885"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PLU11_12615"
     CDS             <1..1460
                     /locus_tag="PLU11_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HPN60018.1"
                     /translation="WNVSSGTARQQAVGGAMASLGGDLSAAYVNPAGLAFYKTGDLVL
                     SPGFNTWNNNATYFSRKEKDNRKYASFGTSGLVIGSGTEKDRKGRNSNSAFALAINRT
                     ANFGSNLLYRGVNNQSSYSHKFLEEINGVGDANAVAGNYPFGTSLAFNTYWIDTIAGG
                     SSGNYQFQTRAPFGTGLIQENVVESRGGITELALAFAGASHDKWFMGFTLGVPFLRYD
                     RTSTFTEADAGTDPNNKFDYAVFTQNLETKGSGINLKLGVIYKPVERVRLGLAFHTPS
                     FYRLTDQYSASITTNTESYQGTMSQASSLLNNNVDGQFTYWMFTPYRVMASGSYVLRE
                     IEDVRKQRGFLTADIEYVNYKASSYSTDPEGDNSQGTKDYLKSLNKAIDNAYKGAFNF
                     RAGGELKFTTIMTRLGLAYYGNPYQDIAGEKGNRFQLSGGLGYRNKGIFIDLTYVHTM
                     GKDVDFAYRLQNRPFSAANLKQTGGNVLLTLGFKI"
     gene            1726..>4001
                     /locus_tag="PLU11_12620"
     CDS             1726..>4001
                     /locus_tag="PLU11_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60019.1"
                     /translation="MRQVTSRLSKDLQTKISIKRVDFSLFDKMHLEGLLLEDRAGDTL
                     LYAGDMKVRITDWFFLKKNIELKYVGLENAVVKFQRTDSVWRQQFIFDYFASSSTDTS
                     KKKSGLQLNLQIAELKNVTFIKKDAWLGEDMRIKVGTLTLDANNLGLTGNRFEINSLQ
                     VTNPDIALYNYSKLKPAGNNQADVPSSEASAAWNEARTVFKIGELKITNGTFSSDRQT
                     DRAPLAYFDGLHIKFTGINAHLNNSSFTGDTVFAKLQITAKERSGLEIKNLSADMKLT
                     PQGMTFNQLDLQTNNSQLRNYFSMSYNDMSELGDFIHKVKMAAQFEDAHIDSDDIAFF
                     APAMSDWNKRIALKGKVRGTVDDLVGKDLLLQAGSNTLLNGDISLTGLPDINQTFIDF
                     KANDFRTVYTDAVQIVPAIQKIKNPDLSKIKYLTFTGSFTGFINDFVTYGTIQTNLGT
                     IRSDLNMKLPAGQDPVYSGSIATDNFRLGEFLGDNNIGLVSMTGTVKGKGFNEKTRNT
                     LVDGDIRYADYKGYRYNNISLKGKLDKKLFDGTASINDAHLQLDMSGVIDFNSSTPRF
                     NLLAKVDKANLKDLNITRDSIDFNGKLNFNFTSSNIDDFLGEARITEAQILKNGTPLP
                     FDSLIVTSAFVDSVKTLNAVSNEFTATIKGNYSLEELPDAFTYFLSRYYPAYIKPPAR
                     LPRNQDISFDINTYYIDEYVKILAPGITGFDNSHFEGTLNLAKNELNFSADVPQLHYK
                     QYNFDNVKFTATGNYDSLV"
BASE COUNT         1105 a          907 c          935 g         1054 t
ORIGIN      
        1 cctggaacgt gtcctccggt acggcccggc aacaggctgt cggcggagcc atggcttccc
       61 tgggtggaga tctttctgcg gcctatgtaa atcccgccgg cctggcattt tataaaaccg
      121 gggatttagt gttgagcccc gggtttaata cctggaataa caatgccact tatttttccc
      181 gtaaggaaaa agataaccgg aagtatgcct cttttggtac atccggactg gtcattggca
      241 gcggaactga aaaagaccgg aaaggaagaa attccaattc tgccttcgcc ctggccatta
      301 accggacggc taatttcgga agcaatctcc tttaccgtgg ggtcaacaac cagagttctt
      361 actcccataa attcctggaa gaaatcaatg gagttgggga tgccaatgcc gtggccggaa
      421 attatccttt cggaaccagt ctggccttta atacctactg gattgatacc atagccggtg
      481 gcagttcggg gaattaccag ttccagaccc gtgctccttt tggcaccgga ctgatccagg
      541 aaaatgtggt ggaatcacgg ggaggaatta cggaacttgc ccttgcattt gcgggtgctt
      601 cccatgacaa atggtttatg ggattcaccc tgggtgttcc ttttctgcgt tatgaccgca
      661 cttccacttt tacagaagct gacgccggta cggatcccaa caataaattt gactatgccg
      721 tttttaccca gaacctcgaa accaaaggtt ccggtattaa cctgaaactg ggcgtgattt
      781 ataaaccggt ggagcgtgtt cgcctgggcc tggcttttca tacacccagt ttttaccgcc
      841 tgaccgatca gtacagtgct tccatcacca ccaatacgga aagctaccag ggtacgatgt
      901 cccaggcctc ttctttactg aataataatg tagacgggca gttcacttac tggatgttta
      961 ccccttaccg ggtgatggcc agtggctcct atgtgttgcg tgaaattgag gatgtgcgta
     1021 aacaaagagg atttctgacc gcggatattg agtatgtgaa ttataaagca tcttcttatt
     1081 caaccgatcc ggaaggcgat aacagccagg gtacaaaaga ttacctgaaa tcgctgaata
     1141 aagcgatcga caatgcctat aaaggcgcct ttaatttcag ggcgggcggc gaactgaagt
     1201 ttaccacgat tatgacccgg cttggcctgg cttattatgg taatccgtac caggatattg
     1261 ccggtgaaaa gggaaaccgg ttccagttgt ccggcggact cgggtaccgg aataaaggca
     1321 tatttatcga cctgacctat gtacatacga tgggtaagga tgtggatttt gcttaccggt
     1381 tgcagaaccg gcctttcagt gcggcaaacc tgaaacaaac cggcggcaat gtattgctca
     1441 cgttaggttt taaaatataa atgaatatta tacgagcata aaaggctgtc ccggcaaagg
     1501 aacagccttt ttttataccc ggcttctgca tgttttgcta aatatccgtt caccccatgg
     1561 attggtattg aatttgccta taggtagcga ttacgtattt ttacaattaa atagctgagt
     1621 ctgaagcggt ttaaaaaaat attaaaatgg tttttcatct gtttactgct gttgattaca
     1681 gggggctata tttttattca atctccctgg ggccaaaact ggattgtgcg ccaggtaacc
     1741 agccgccttt ccaaggatct tcagacaaag atcagcatca aacgggttga cttttcctta
     1801 ttcgacaaaa tgcacctgga aggtttgttg cttgaagacc gtgccggaga taccctgttg
     1861 tatgcaggag atatgaaagt gcggatcacg gactggtttt ttcttaaaaa aaatattgaa
     1921 ctcaaatatg tcggcctgga aaatgcggtg gtaaaattcc aacgcaccga ttcagtctgg
     1981 agacagcaat ttattttcga ttattttgct tcctcctcca ccgataccag taagaaaaaa
     2041 agcgggctgc agctgaacct gcaaatagcg gagttgaaga atgtaacgtt tatcaagaaa
     2101 gacgcctggc tcggagagga tatgcggatc aaagtgggaa cccttacgct ggatgccaat
     2161 aacctgggac tgaccggcaa ccgttttgaa attaattcgc ttcaggtcac caatccggat
     2221 atcgccctgt acaattattc caaacttaaa cctgccggca ataaccaggc agacgttcct
     2281 tccagtgagg catctgcagc ctggaatgaa gcccggactg tttttaagat cggggaacta
     2341 aagatcacca atggcacttt tagttctgac cgtcagacgg acagagcgcc acttgcctat
     2401 ttcgatggct tacatataaa attcaccggc atcaatgccc atctgaataa ttcctctttt
     2461 accggggata ccgtttttgc aaagcttcag atcacggcaa aagagagaag cggactggag
     2521 ataaagaatc tgtcagccga tatgaagtta accccgcagg ggatgacgtt taatcaactc
     2581 gacctgcaga cgaataacag tcagctccgg aattattttt ccatgtcgta taacgacatg
     2641 agtgaactcg gggattttat tcataaagtg aaaatggcgg cacagtttga agacgcccat
     2701 attgacagtg atgatatcgc cttttttgca ccggcgatgt ccgattggaa taaacggatt
     2761 gccctgaaag gaaaagtgcg gggaacggtg gatgacctgg tgggaaaaga tctgttgctg
     2821 caggccggca gcaatacctt actgaatggc gatatctctc ttacagggct gccggatatc
     2881 aaccagactt ttatcgattt taaagccaat gatttccgca cggtatacac cgatgcggta
     2941 caaattgtcc cggccataca aaaaatcaaa aacccggacc tcagtaaaat caagtacctc
     3001 actttcaccg gaagttttac gggattcatc aatgattttg taacctatgg taccattcag
     3061 accaatcttg gtaccatccg cagtgacctg aatatgaaat tgccagccgg acaggatcct
     3121 gtttattccg gatccattgc tactgataat ttcaggctgg gtgaattcct cggcgataat
     3181 aatatcgggc tggtatccat gacagggacg gtgaaaggaa aaggctttaa tgaaaaaaca
     3241 aggaataccc tggtagatgg tgatatccgg tatgccgact ataagggcta tcgttataat
     3301 aatataagtt taaaaggaaa gctggataaa aaactgtttg acggcacggc ttcaatcaat
     3361 gatgcccacc tgcagctgga tatgtccggt gtgattgatt ttaacagcag taccccccgc
     3421 ttcaacctgc tggcgaaagt ggacaaggcg aacctgaaag acctgaacat cacccgtgac
     3481 agtattgact ttaacggcaa gcttaatttt aattttacca gtagcaatat cgacgacttc
     3541 ctgggagaag cccgtatcac cgaggcgcag attttgaaaa acggtacccc cctgcccttc
     3601 gattcgctga tcgtgacctc tgcttttgtg gatagtgtta aaaccctgaa tgcagtttcc
     3661 aatgaattta cggcaaccat taaaggcaat tacagcctgg aggaattgcc ggatgctttc
     3721 acctattttc tgagccggta ttaccctgct tatatcaaac ccccggcccg gctgccccgt
     3781 aaccaggata tcagctttga tatcaatacc tattatattg atgaatatgt aaaaatactg
     3841 gccccgggta tcactggttt tgacaacagt cattttgaag gcaccctgaa cctggctaaa
     3901 aatgagctca atttttcggc tgatgtgccc cagttacatt ataagcagta taactttgat
     3961 aatgtaaaat tcacggctac cggtaattac gacagccttg t
//
LOCUS       DAOPMW010000315         3971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_26981_length_3971_cov_5.089888, whole genome shotgun sequence.
ACCESSION   DAOPMW010000315 DAOPMW010000000
VERSION     DAOPMW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26981_length_3971_cov_5.089888"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLU11_12625"
     CDS             complement(<1..248)
                     /locus_tag="PLU11_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60020.1"
                     /translation="MKFYNLLIKYRLWVGLALIALGAYVNFASSFWPAFPLYLVGVIL
                     IAGHFFFGPLRLIQEYMESGDMDGAEKVINSIKFPNLL"
     gene            609..1361
                     /locus_tag="PLU11_12630"
     CDS             609..1361
                     /locus_tag="PLU11_12630"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HPN60021.1"
                     /translation="MPFTLAHPLLPVLIKKAVPRLSLSALIAGSVIPDMENFFKMQDA
                     WSFGHGLYGLLLLDLPAGLLLCFVFHLLLRNSFIANLPWYYRNRFASYTEFDWTRYAW
                     ANKTVVLISLIAGGLSHLGWDAFTHKDGFFVQLFPLLSRKIILAGTAYPVHYLLQLIS
                     SVLGIWLLYRYIRLLPSKPASEHLVKPDRLYRLTFLGILIVLLLVRLLGWPDLNTYLG
                     LWRAGMGALFYTWVIVSFIFYKRDMRVSNVPL"
     gene            1374..3050
                     /locus_tag="PLU11_12635"
     CDS             1374..3050
                     /locus_tag="PLU11_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPN60022.1"
                     /translation="MIQFRKTNLFYGGLLLLILSSVLDLPAQNNAWLVDSTVQLIDGR
                     SPRFSLVKPGDTIFLTAGNRDKLLIRNLSGSSAQPVVIMNRGGTVHINTAHYYGLSLS
                     GCRYLRISGQGNSPDFYGFQINRVANGAGIGLSAFSSDIEIDHLLIENCFTAGIYAKT
                     DPDCTFLATREKFTQFNTSIHDNSIAHVGNEGMYIGSSFYSGVTLNCNGKDTLVYPPV
                     LNGVQVFNNKVAYTGWDAIQVSSAVTACAVYGNTIEYDSQSETDSQMSGILIGGGSKC
                     DCSHNYISNGKGDGIEDHGLGGNRIYNNIIVNAGRSYFPGNPAKMKHGIFVSDVSVQK
                     DSAFTIVFNTIIQPKSDGIRFQSVKSKNSLIASNLVADPGNYDYYELGTTFFRGKDAY
                     VMLPSPLSSIQLQHNYFTPSVANARLSAAFVPMTGSPLINQGYQYNLDITDDFLKQKR
                     PCGDWYDIGAIEYTGGADSLISFSGFNPVAFPDPAHSEITIRYLNKPAFPSVITIYSI
                     QGTRFLQQSVSSPVPGIQEVQFPINRLPAGLYLFTIRTGSEISRGKFMKL"
     gene            complement(3054..>3971)
                     /locus_tag="PLU11_12640"
     CDS             complement(3054..>3971)
                     /locus_tag="PLU11_12640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60023.1"
                     /translation="DDGTPELLKQYKEAIVLHENGRNGKIGAMNRGMKLVNTPIVIFS
                     DANTMLGKESIRRIVRLFETPTVGCVSGEKRITDKDTDVASGAGEGLYWKYESTLKKW
                     DAELYSVVGAAGELFAIRTSLYREVEKDTLLDDFIISLRVAQEGYTIQYDPEAYAVES
                     ASANVKEELKRKIRISAGGIQSVVRLRSLLNIFKYGTLSFQYISHRVLRWTLTPLCLL
                     LLIPAGFALAWSEGLFSPGVYMILFWLQLAFYIMALTGWFLENRSTSIKVLFVPYYFF
                     IMNLSVVLGFMRYMKKSQTVNWERAKRAA"
BASE COUNT         1022 a          874 c          905 g         1170 t
ORIGIN      
        1 tataacaggt tcggaaattt gatcgaatta attactttct ccgctccatc catatccccg
       61 ctttccatat attcctggat cagccgcagc ggtccgaaga agaaatgccc ggcaatcagg
      121 atcactccga ccaggtataa aggaaaggct ggccagaaag aggaagcaaa atttacataa
      181 gcacccagtg caatcagcgc taatccgacc caaagccggt atttgatcag taagttgtaa
      241 aatttcattt tttgtttatt tacccggccg gtttgattgc cggaccagga attcgttaat
      301 aattaattaa ttatcagtgc tttatatgca aaaggcagat gccggaccct gcaaaagtat
      361 tgaaaactcc gcttacccgg tacaatgaaa agaatcggcg ttttcgctcc tttatggctg
      421 ccagaaaagg agtatccggg atgttgaatg actggatttt acaatcaatg ggcagtagta
      481 tctgtttaac ttatatatgc ttaagtgtct gtacttagat atgaaattca ggagggcatt
      541 tttgaatcaa ctggtttatt taattgtatt ttaccacagg tgtttatatt aaaaaaggcg
      601 ctgaatcaat gccatttacc ttagcacatc ctctcttgcc ggttttaata aaaaaagccg
      661 ttcctcgttt atcactgtcc gccctgattg ccggaagcgt gattccggat atggagaatt
      721 ttttcaaaat gcaggatgcc tggtcattcg ggcacggact gtatggactg ctcctgttag
      781 atttgccggc aggattgctg ctttgttttg ttttccattt gttattgcga aattctttta
      841 ttgccaatct tccctggtat taccgcaatc ggtttgcttc gtataccgaa tttgactgga
      901 cccgttatgc atgggctaat aaaacagtgg tgctgatttc attgattgca ggtgggttgt
      961 ctcatcttgg ctgggatgca ttcacccata aggatggatt ttttgttcag ctttttccac
     1021 tactcagccg aaaaattata ttggccggta ctgcttatcc ggttcattat cttttacaac
     1081 tgatatcaag tgtgctgggt atctggctgc tgtatcggta tatccggctc ttaccgtcaa
     1141 aaccggccag tgaacacctc gtaaaacctg acaggttata ccggttgacg ttcctgggta
     1201 tactgatcgt attattattg gtacgcttgc tgggatggcc ggacttaaat acttacctgg
     1261 gactttggag agccggaatg ggtgctcttt tctatacctg ggttattgta tcctttattt
     1321 tttataaacg cgacatgcgg gtcagtaatg tgcccttgta aacagatcct atcatgattc
     1381 aattccggaa aaccaattta ttttatggag gcctgctact cttaatactg tcatctgttt
     1441 tggatttgcc ggcgcagaat aatgcatggt tggtggattc aacagtacaa ctaatcgatg
     1501 gccgaagtcc gcgtttcagc ctggtgaagc cgggggatac catttttctg acggcaggaa
     1561 accgggacaa actactcatc cgaaatcttt ccggatcatc tgcacaaccg gtggtcatta
     1621 tgaatagggg aggaacggtg catataaata ccgcacatta ctatggcctc tccttatccg
     1681 gctgccggta tctccgtatt tccggacaag ggaattcacc tgatttctat ggcttccaga
     1741 tcaaccgggt ggcaaacgga gccggtattg gcctcagtgc cttcagcagc gatattgaaa
     1801 ttgatcatct gctgattgaa aactgtttca ccgctggtat ctatgccaaa actgacccgg
     1861 attgcacgtt cctggccacc cgggaaaaat ttactcagtt taatacaagt attcatgata
     1921 attccatagc ccatgtgggc aatgaaggaa tgtatatcgg cagttctttt tattccgggg
     1981 tcaccctgaa ttgtaatggg aaggacaccc ttgtttatcc cccggtatta aacggggtgc
     2041 aagtgtttaa caacaaagtg gcgtataccg gttgggatgc gattcaggtc agttcagcag
     2101 ttactgcctg tgcggtctat ggtaatacga ttgaatacga tagtcagtca gaaacggata
     2161 gtcagatgtc cggtatcctg atcggcggcg gttctaaatg tgattgcagt cataattata
     2221 tcagcaatgg aaagggcgat ggtattgaag atcatggcct gggtggtaac cggatttata
     2281 ataatattat tgtcaatgca ggacgttctt atttccccgg caatcctgcc aaaatgaaac
     2341 acggcatttt tgtatcggat gtatctgtac aaaaagactc ggcttttacc attgtgttta
     2401 atacgatcat tcagcccaag tcagatggta tccggtttca gagtgtgaag agtaaaaaca
     2461 gtctgatcgc ttccaacctg gtggcggatc cgggtaatta tgattactat gaattgggta
     2521 ctactttttt cagaggtaaa gatgcttatg tgatgttgcc gtctccttta tcctccattc
     2581 aattgcaaca taattatttt acaccgtctg tcgccaatgc ccgtttgtcg gcagcatttg
     2641 ttccaatgac cggatctcca ctgatcaatc agggctatca gtataacctg gacattacag
     2701 atgattttct gaagcagaag cgtccctgtg gagattggta tgatatcggg gctatagaat
     2761 atacaggtgg tgcggattcg ctgatttctt tttcaggatt taatccggtg gcttttccgg
     2821 atcctgccca ttcagaaatt accatacgtt atctgaataa accggcattc ccctcagtga
     2881 ttacaattta ttccatacaa ggaacccggt ttcttcagca aagcgtatcc tccccggttc
     2941 cgggtataca ggaggtgcag tttccaatta acagacttcc tgccggtctg tatcttttta
     3001 ctatccgcac aggatcggaa atcagccggg gtaagtttat gaagttataa tgctcaggcg
     3061 gctctttttg cccgttccca gtttaccgtt tgtgactttt tcatataccg cataaaaccc
     3121 agtacaacgg agaggttcat gatgaaaaaa taatacggca caaacagcac tttgatgctg
     3181 gtggatctgt tttcgagaaa ccaacctgta agggccatga tatagaaggc aagttgcagc
     3241 cagaaaagaa tcatatacac gcccggacta aacaatcctt cggaccaggc gagcgcaaaa
     3301 ccagccggga tgagcagtaa caggcagagc ggggtaaggg tccagcgcag taccctgtga
     3361 gagatatact ggaaagaaag ggtgccgtac ttaaaaatat tcagcaggga gcggaggcgg
     3421 accaccgatt gtatcccgcc ggcagatatc ctgattttac gtttgagttc ctcctttaca
     3481 ttagcagagg cggattccac ggcataggct tcggggtcat actgaatggt atatccttcc
     3541 tgggccacgc gtaaggagat aataaaatca tccagcaacg tgtctttttc cacttcgcgg
     3601 tacagggatg tacggatggc aaacaactca ccagctgcac cgaccacaga gtataattct
     3661 gcatcccatt ttttgagcgt ggattcatat ttccagtaca gtccttctcc tgcgccggag
     3721 gccacatccg tatctttatc cgtgattctt ttttcaccgg atacacagcc tacggttggg
     3781 gtttcaaata aacggacaat ccggcggatt gattctttgc ccagcatggt attggcatca
     3841 ctgaatatca caatcggggt gttcacgagt ttcatacccc ggttcatggc cccaatttta
     3901 ccattacgcc cgttttcatg taatacaatc gcttccttgt attgctttaa caattccggg
     3961 gtgccgtcat c
//
LOCUS       DAOPMW010000316         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27028_length_3966_cov_2.906162, whole genome shotgun sequence.
ACCESSION   DAOPMW010000316 DAOPMW010000000
VERSION     DAOPMW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27028_length_3966_cov_2.906162"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..2059
                     /locus_tag="PLU11_12645"
     CDS             149..2059
                     /locus_tag="PLU11_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPN60024.1"
                     /translation="MKKYQCFALLFLFTGTLFAQQNAATLNLIPVPVSIKQGQGQCML
                     PYNATIYYNGQEDARKIVNQLADRLNTGGNLIQIKKGKSALPGSINYVLTNEPGIPAE
                     GYQLIVSQKNITLKASNTAGLFYGTQTLLQLLPAAVEFPERQKPASSWILPVVTIEDH
                     PRFGWRGLMLDVSRHFFTVAQVKDYIDQMVKYKFNLLHLHLTDDQGWRLQIKSLPKLT
                     EVGAWRVERTGTFGTLSKPLPGEKATYGGFYTHEDIKELVKYAADRFVNILPEIDVPG
                     HSLAAIAAYPELSCTPGEYYVSPGDRFMIWPGGGQHFYGTLDNTICPALEKNFEFLDK
                     VFTEVAELFPFGYIHMGGDETARNFWEKSEQIKALMQEKGLKNLDEVQSYFVKRVEKI
                     INSKGKKMIGWDEILDGGLAPNAAVMSWRGMKGGIEAAKQGHEVVMSPTDFAYIDYMQ
                     GDASIEPPVYATLRLKKAYQFEPIPEGVNPALIKGGQANLWTEQVYNTRHLQYMTWPR
                     GMAIAEALWSPKEKRNWSDFSGRVVSHFTRLDMAGVKYAPSIYDPIITAEKKDTATVM
                     IKLSTELDDLKIHYSFDNSFPDTWYPVYTEPLLVPKDASTMKLITSRNGKITGRMMSI
                     PVTDLKKRAGIK"
     gene            complement(2290..>3966)
                     /locus_tag="PLU11_12650"
     CDS             complement(2290..>3966)
                     /locus_tag="PLU11_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60025.1"
                     /translation="HLLCHGYKVEPEIGAPYVIVYGQDISDRIRIEEDLYRAKMQSEK
                     NAQAKESFLAKVSHEIRTPMNGILGVADLLSKTNPDEQQNRYINVLKMSAQNLLAIVN
                     DVLDIEKILAGKMLLEDIPFDLQKTVDLLKDIFSSEASVRNNNFSIVNELEPGTQFNG
                     DPFRISQVLSNLLSNAVKFTNGGIITLRISRVEEDHQNSRICFRVEDNGIGIPAEKIN
                     EIFEPFSQVDYSAGSGMLGTGLGLTICREIAELMNGSLVVESKLGKGSVFTFTVPLQK
                     KQPASTIPVEPPAGKTGKSLEGYHILLVEDMELNLFIIQEMMRDWELQPDIARNGEEA
                     VLFSLQKKYDLILMDIQMPVMNGVEATTRIRTDQSNPNHHTPIIAISANAFESDKVSY
                     FKAGMNGVLSKPFDADKLYIVISENLDEGKTTQAQVLSAGTPVLVSPQVKINLSYLLQ
                     IGKNNRGFVGMMLQSFRDSSNEIIADMEHSLERKDWTKTAQLVHKLKFSLNVMGAGSL
                     DEEVKWIESNTRHPLPENEHEMQVRIYAFITVIRELDQHAGQLIESGEWS"
BASE COUNT         1080 a          926 c          903 g         1057 t
ORIGIN      
        1 gttcgaggtt ggaggttcga ggttggaggt tggaggttcg aggttggagg ttggaagttc
       61 gaggttggaa gttcgaggtt gacagggatg tcagcctttt tttattgctg ggattatata
      121 gtaaattgcc ggacaacacc attgcaccat gaaaaaatat caatgctttg ccctattgtt
      181 tctcttcacg ggaacattgt ttgctcagca gaacgcggct acactgaacc tcattccggt
      241 tcccgtatca attaaacaag ggcagggcca gtgtatgctg ccctacaatg ccacgattta
      301 ttataatgga caagaagatg cccggaaaat cgtgaaccag ctggctgacc ggttgaatac
      361 cggcggaaac ctgattcaga ttaaaaaagg gaaatcagcg ttacctggtt caatcaatta
      421 cgtgctgacc aatgaacccg gaatacctgc agaaggatat cagctgatcg tcagccaaaa
      481 aaacatcaca ctcaaagcca gtaacacggc aggattattc tatggcacac agaccctcct
      541 gcaattgttg cctgcagcag ttgaatttcc tgaaagacaa aaaccggcaa gttcatggat
      601 ccttcctgtt gtgactattg aagatcatcc ccgctttggc tggcgcggac tgatgctgga
      661 tgtgtcccgg catttcttca ccgtggccca ggtaaaagat tatatcgacc agatggtgaa
      721 gtataaattc aacctgctgc acttacatct cacggatgac cagggctggc gcttgcagat
      781 aaagagttta cctaaactga cagaagtagg tgcctggcgg gtggaaagaa ccggcacatt
      841 cggaacattg tctaaacccc tgccgggtga aaaagctact tatggcggat tctataccca
      901 tgaggatata aaagaattag tgaaatatgc ggctgaccgc tttgtcaata tacttcctga
      961 aatagatgtt ccggggcata gcctcgccgc tattgctgct tacccggaac tgtcctgtac
     1021 accgggtgaa tattatgtaa gtccgggtga tcgttttatg atctggcccg gtggcggtca
     1081 gcatttctac ggcacactgg ataataccat ttgtccggca cttgaaaaaa actttgaatt
     1141 cctcgataaa gtatttactg aagtagccga actttttcct tttggataca tccacatggg
     1201 cggagatgaa acagcccgca acttctggga aaaaagtgaa cagatcaaag ccctgatgca
     1261 ggagaaagga ttaaaaaacc tggatgaagt gcaaagctat tttgtaaaac gggtggagaa
     1321 aatcatcaac agtaagggaa agaaaatgat cggctgggat gagatccttg acggaggtct
     1381 ggctcccaac gcggccgtga tgagctggcg agggatgaaa ggaggcattg aagccgctaa
     1441 gcaagggcat gaagtggtga tgagccctac tgactttgct tatatcgatt atatgcaggg
     1501 cgatgcttcc attgaaccgc ctgtatatgc cacccttcgt ttaaaaaaag cctaccagtt
     1561 tgaacctata ccggaaggcg tcaatccggc ccttattaaa ggcggtcagg ccaatctctg
     1621 gaccgaacag gtatacaata cccgtcatct gcaatatatg acctggccac gggggatggc
     1681 gattgccgaa gcactctgga gtccgaaaga aaaaagaaac tggagtgatt tttcgggaag
     1741 agtggtcagc catttcaccc ggctggatat ggccggggtg aaatatgctc ccagcatcta
     1801 cgacccgatc atcactgcgg aaaagaaaga caccgccacc gtgatgatta agttaagtac
     1861 agaactggat gatttaaaaa ttcattacag ctttgacaac tcattccccg acacctggta
     1921 tccggtttat accgaaccgc tgctggtacc caaagatgcg tcaacaatga aactcatcac
     1981 gagccggaat ggaaaaatta ccggccgtat gatgagtatc ccggtaaccg atttgaaaaa
     2041 aagagccggt attaaataag aaggctatgt gtatgcgaaa agaaacggct atgtatatac
     2101 ccttatttta caggaaaaaa cagttcgcga ttttatttct attaaccagc cccttacttt
     2161 tatagtcaag gataaggtcc ggtttctacc ggcaggttta atggttatat gttcaagatt
     2221 caaaaaagac tggtttttta aaaccagttt tttttattct cctaccgttc ccttttaatt
     2281 gaccgatatt caactccact ctccggactc tatcagttgc ccggcatgct gatccagttc
     2341 ccggataacg gtaataaaag catagatacg cacctgcatt tcatgttcat tctccggcag
     2401 cggatggcgg gtattgcttt caatccattt tacttcttca tccaggctgc ctgcacccat
     2461 tacattgagg gaaaatttaa gtttatgtac caattgagcc gttttggtcc agtctttccg
     2521 ttccagtgaa tgctccatat cagcgatgat ctcattggag gaatccctga atgactggag
     2581 catcataccc acaaagcccc ggttgttttt tcctatttgt aaaagatagg aaaggttgat
     2641 cttcacctgg ggcgaaacga gaaccggtgt tccggcagac agaacctgtg cctgtgttgt
     2701 tttcccctcg tccaggtttt ctgaaatgac tatgtaaagt ttatccgcat caaatggttt
     2761 tgataaaacc ccattcatac ccgccttgaa ataggatact ttatcggact caaacgcgtt
     2821 ggcagaaatg gcaatgatgg gtgtatgatg attgggattg ctttgatcag tcctgatccg
     2881 ggtcgtggct tccaccccgt tcattaccgg catctgaata tccatcagga tcaggtcgta
     2941 ctttttctga agggaaaaca ggaccgcttc ttctccgttc ctggcaatat caggttgcag
     3001 ttcccagtca cgcatcattt cctgtataat aaacaggttt aactccatat cttccacaag
     3061 cagtatatga tatccttcca ggcttttccc ggttttaccg gcaggcggct caaccggaat
     3121 cgtagatgcc ggctgcttct tctgcaaagg caccgtaaac gtaaacacag agcctttgcc
     3181 cagtttactt tccaccacca ggcttccgtt catcagttcg gctatttcgc ggcagatcgt
     3241 aagtccgaga ccggttccaa gcattcctga gccggcacta taatccacct gggaaaaagg
     3301 ttcaaaaatc tcattgatct tttccgccgg tattccaatc ccgttgtcct ccacccggaa
     3361 acagatccgg ctgttttgat gatcttcttc cacccggctg atccggagtg ttataatccc
     3421 tccattggta aattttaccg cattggaaag aagattactt aacacctggg aaatacggaa
     3481 aggatctccg ttaaactgtg taccgggctc cagttcgttg acaatggaaa aattattatt
     3541 ccggaccgat gcttcgctgc tgaaaatatc cttcaagaga tcaacggtct tctgcaggtc
     3601 aaaaggaata tcctccaaca gcatttttcc cgccagaatt ttttcaatat ccagcacatc
     3661 atttacaatg gccaggaggt tctgggcaga catcttcagg acattaatat accggttctg
     3721 ctgctcatcg gggttggtct tggataacag gtcggccacc cccaaaattc cattcatagg
     3781 ggtgcgtatt tcatgactca ctttagcaag aaaggactct ttcgcctggg cattcttttc
     3841 cgactgcatt ttagcccggt aaagatcctc ttctatacgt atgcggtcac tgatatcctg
     3901 accatagaca attacataag gcgcccctat ttcgggctcc actttatacc catgacataa
     3961 caaatg
//
LOCUS       DAOPMW010000317         3962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27061_length_3962_cov_8.659073, whole genome shotgun sequence.
ACCESSION   DAOPMW010000317 DAOPMW010000000
VERSION     DAOPMW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27061_length_3962_cov_8.659073"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLU11_12655"
     CDS             complement(<1..185)
                     /locus_tag="PLU11_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPN60026.1"
                     /translation="MIDWYSIVFFLLTLLLMGFFAGIEMAFYSANRLSLELKKKQGDS
                     NMEILSEFLESPARFLGT"
     gene            complement(274..879)
                     /gene="gmk"
                     /locus_tag="PLU11_12660"
     CDS             complement(274..879)
                     /gene="gmk"
                     /locus_tag="PLU11_12660"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPN60027.1"
                     /translation="MPGKSAKIIIIAAPSGAGKTSVVRHLLKVMPEQLSFSISAATRK
                     PRNNEKDGVDYYFLSVEDFEAKIAADDFVEWEMVYEGKYYGTLKEEVARIWNEGKAPL
                     LDVDVQGGLHVQDKFRGQTLSLFIEPPSIRELERRLRSRGTETEESLKARVSKAAYEL
                     TFKDQFDHVILNDELPRACSEAETLIRHFLMNGSNWPQISK"
     gene            complement(917..3109)
                     /locus_tag="PLU11_12665"
     CDS             complement(917..3109)
                     /locus_tag="PLU11_12665"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HPN60028.1"
                     /translation="MKKLLFLVVLFACTAGQSQITTRIELSNALFKTGDNLEWSKPGL
                     DDHDWKPIKPQYNWDKQGFADYDGFGWYHFHFELSSLIKLNSFWADSVRLFLAKIDDA
                     DEVYLNGKRISKTGRFPDDPGGYSTQWDRWRDLRIATNDPVLQWDKDNVLAIRVYDGG
                     GIGGMFGGIPGISMLQLKDMASLEYKNKALHFRHQLPEKITGMLTIKITDPATQTEVK
                     NYSQTITVEPNSDYTLPLPTDPKTRQEIAASFMENKSGQLVYLLTVPPYLLTPAPAAA
                     PRINNASVFGVRPGSPFLFKIAATGDKPLQYSATGLPKGLKLDPATGIITGSLQRAGE
                     YTVQLLVKNKKGRARKNFTIKCGDLLALTPPMGWNSWNCWGLSVSSEKVKASAQALID
                     KGLIDHGWSYMNIDDGWEAPKRNEKGEIVTNDKFPDMKALGDWLHSKGLKFGIYSSPG
                     PLTCGGYLGSYQHEKQDADMYTQWGIDYLKYDWCSYGQIHGGKDTSTASYIKPYTTMQ
                     TALRTQPRDIVYSLCQYGMKDVWKWGHEVDGNCWRTTGDIEDTWESMYAIAFNQTAMQ
                     PYAKPGRWNDPDMLIVGNVGWGENLHPTRLNADEQYTHISLWCLLASPLLIGCDIASM
                     DDFTLNLLSNDEVNAINQDAAGKQAIRVIKRENYQVWVKDLEDGSKAIGIFNVGEKYE
                     NLRVDWTELGLEGNYNIRDLWRQKELGSGNSYSSRIPPHGVSLIKISR"
     gene            complement(3149..3784)
                     /locus_tag="PLU11_12670"
     CDS             complement(3149..3784)
                     /locus_tag="PLU11_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60029.1"
                     /translation="MNSNREQIFRWEIIPLAGIPLFILLYFIAARFYPGGHPGNPQAE
                     GFSWMHNYWCNLLNEQAINGERNTGRPVAMAGMIVLCISLATFWFYYPVKPGIPRKLQ
                     WLIRISGPLAMGTALFITVFDHDGVTNIASLLGLIAVTGTLVILYKTKQTWLFRLGLL
                     NLLLVAVNNLFYYTPSLLHLLPVVQKLSFISVLGWIWLCGYCSGHSKWPEQ"
BASE COUNT          977 a         1009 c          938 g         1038 t
ORIGIN      
        1 gtgcccagga aacgggccgg tgattccagg aattcggaga gaatctccat attactgtcc
       61 ccctgtttct ttttcaactc caggctcagc cggttggcac tgtaaaaggc catttcaatt
      121 cctgcgaaaa aacccatgag caggagcgtg aggaggaaaa acacaataga gtaccaatcg
      181 atcatgggac aaatatagta aatggagaat agagaataga aaatggggcc tcctgaaggg
      241 ccaatcttaa gactataaat tattgattat taattattta ctgatttgtg gccaattact
      301 tccattcatc agaaaatgac gaatcagggt ctctgcctca ctacaggcac ggggcagttc
      361 gtcattcagg atcacatgat caaactgatc tttgaacgtg agctcgtagg cggctttgct
      421 tacgcgggcc ttgagggatt cttcagtttc tgttccgcgt gaacggagcc ggcgctccag
      481 ttcccgaatg gatggaggtt caataaataa ggaaagggtt tgtcccctga atttatcctg
      541 cacatgtagt ccgccttgta catccacatc cagcagaggg gctttgcctt cgttccagat
      601 ccgcgctact tcttctttaa gtgtgccgta atattttcct tcatacacca tttcccattc
      661 cacaaaatca tcggctgcga tctttgcttc aaaatcttcc acggaaagaa aataatagtc
      721 cacgccgtcc ttttcattat tccggggttt tcgcgtggcg gctgatatgg aaaaggaaag
      781 ctgctccggc atcactttta gcaaatgacg aaccacggag gtcttacccg ccccggaagg
      841 cgcggcaata atgatgattt tagcagattt acccggcata taaaggcggc aaaggtaaag
      901 attaatcggg gcaaactcag cggctgattt tgatcagact cactccatgc ggaggaatac
      961 ggctggagta actgttgcca ctgcccagtt ccttttgccg ccacagatcc cggatgttat
     1021 aattgccttc cagtccgagt tccgtccagt caacccgcag gttttcatat ttctccccca
     1081 cattaaaaat accaatagcc ttgctgccgt cttccagatc cttaacccat acctggtagt
     1141 tttctctttt aatcacccgg atagcctgct tgccggctgc atcctggtta atcgcattta
     1201 cttcatcatt gctgagcaga ttcaatgtga aatcatccat actggcaata tcacaaccga
     1261 tcagtaaagg agaggccagc aggcaccaga gactgatatg ggtatattgt tcgtctgcat
     1321 taagccgtgt cgggtgcagg ttctcccccc agcccacatt gcccacgatc agcatatcgg
     1381 gatcattcca gcggccgggc ttggcatagg gttgcatagc ggtctgatta aaagcaatgg
     1441 catacatgct ttcccaggta tcttcaatat ccccggtagt gcgccagcaa ttgccatcca
     1501 cttcatgtcc ccatttccac acgtctttca ttccatactg acataaacta tacacgatat
     1561 cgcggggttg ggtccgcagg gcggtctgca tcgtagtata aggttttata taggaagcag
     1621 tactcgtatc tttcccgcca tggatctgtc cataactgca ccagtcatat tttaaataat
     1681 cgatccccca ttgggtatac atatcggcat cctgcttttc atgctggtaa gaaccgagat
     1741 aacctccgca ggtgagcgga ccaggagagg agtaaatacc gaatttcaat cccttgctgt
     1801 gcagccagtc gcccagggct ttcatatcgg ggaacttgtc attggtaacg atctcccctt
     1861 tttcattacg ctttggcgct tcccatccgt catcaatatt catgtaactc cagccatgat
     1921 cgatcagacc tttatcaatc aaggcctggg ccgaggcttt cactttttcc gaactcacac
     1981 tgagtcccca gcaattccag ctgttccagc ccatgggtgg agtgagcgcc aggaggtctc
     2041 cgcatttgat ggtaaagttt ttgcgggccc ggcctttttt attcttcacc agcagttgaa
     2101 cggtatactc cccggcccgt tgcagcgaac cggtaatgat gcctgtggcc ggatccagtt
     2161 ttaatccttt gggcaaaccg gttgcactat actgcagtgg cttatccccg gtggcggcta
     2221 tcttaaacag aaagggcgag cccggacgaa ccccaaatac cgacgcgtta ttgatcctgg
     2281 gtgctgctgc cggagctggg gtaagcaggt aaggtggaac ggtcaacaga taaaccagtt
     2341 gcccgctttt attttccata aaactggccg caatctcctg ccgggtcttg gggtctgttg
     2401 gtaacggtaa agtatagtcg ctgttaggtt ccactgttat cgtctgactg taatttttga
     2461 cttcggtctg tgtggccggg tcggtgattt ttatcgtcag catcccggtg attttttccg
     2521 gcagctggtg acgaaaatgc agcgctttgt ttttgtattc caggctggcc atatccttca
     2581 gctggagcat gctgatgccg ggtatgccgc caaacattcc cccgatacca cccccgtcat
     2641 atacccggat ggccagtaca ttgtctttat cccactgcag caccggatca ttcgtggcga
     2701 tccgcaggtc gcgccagcgg tcccactggg tactgtagcc ccccggatca tccggaaaac
     2761 gacctgtctt gctgatccgt ttaccattca ggtacacttc atcggcatca tcgatcttgg
     2821 ccaggaagag acggacgcta tcggcccaaa agctattgag ctttatcagt gaggataatt
     2881 caaaatgaaa atgataccag ccaaaaccat cgtagtcggc aaaaccctgt ttgtcccagt
     2941 tgtactgggg tttgataggt ttccagtcat gatcatccag tcccggttta ctccattcga
     3001 ggttatcccc ggttttgaac agggcattac tgagttcaat ccgggtggtg atttgtgatt
     3061 ggcctgcggt acaggcaaag aggactacta aaaagaggag ctttttcata tcagcgtttt
     3121 gcagcttaaa attagtggtt tctcgcggtt actgttctgg ccacttagag tggccagaac
     3181 agtaaccgca taaccatatc caccccaaca ccgaaataaa actcagtttt tgtaccactg
     3241 gcagcagatg aagtaacgaa ggagtgtaat aaaacagatt gttaaccgct acaagcaaca
     3301 ggttcaacag cccgagccgg aataaccagg tctgttttgt tttatagagt ataaccaagg
     3361 tacctgttac ggcaatcagt cccagtaaag aagcgatatt ggtcacccca tcatgatcaa
     3421 agaccgtaat aaagagtgcc gtccccatcg ccaacggacc gctgatccgg atcagccatt
     3481 gcaatttccg gggaatgccc ggctttaccg gatagtaaaa ccagaaggta gcgagactga
     3541 tgcataatac gatcataccg gccattgcca ccgggcggcc tgtgttccgt tctccgttta
     3601 tagcctgttc attgaggaga ttgcaccaat aattgtgcat ccagctgaat ccctcggcct
     3661 ggggattacc gggatggcca ccgggataaa aacgggcggc aataaaatag agcagaatga
     3721 acagtggaat tcctgcaagg gggatgattt cccaacggaa tatttgttca cggtttgagt
     3781 tcataactat tgtttggtaa ttgttctggc cacttcgagt ggcctgaaca attaccacca
     3841 ctactgcgcc acgctgttga cttcaatcca gtacctatcc gggtcctgta aatagatctg
     3901 aaggatgcca tcgggacgaa catgcacttt gccgggcgtg ccgggccagt cggtgaacgg
     3961 aa
//
LOCUS       DAOPMW010000318         3962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27064_length_3962_cov_6.688764, whole genome shotgun sequence.
ACCESSION   DAOPMW010000318 DAOPMW010000000
VERSION     DAOPMW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27064_length_3962_cov_6.688764"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..2251
                     /gene="glgB"
                     /locus_tag="PLU11_12675"
     CDS             212..2251
                     /gene="glgB"
                     /locus_tag="PLU11_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPN60030.1"
                     /translation="MSAKKNTTAADKARKKKSVASLKDKSRQPVPSYSKKYEEENFVD
                     TSLLVWNYSLLTDEDIQNYQQGTNYQLYKKFGSHSIEVNNTWGMYFCVWAPNATAVSV
                     KGNFNDWKNHEYALYPRWDKSGVWEGFIPGFKLGEAYKYHITGFGKKELDKGDPFANF
                     WEKRPQTASITWDMHYDWEDKEWMKKRKKHNSLEAPWSVYELHLASWMRPDKNDEERY
                     NSYDDIRDRLVPYLKETGFTHVEFMPLMEHPFDGSWGYQCTGFFAATSRFGDPQGLMR
                     LIDALHQAEIGVLLDWVPSHFPYDAHGLFMFDGTHTYEYADMRKGFHPDWNSYIFNYK
                     RGEVKSFLISSARFWFDLFHIDGIRVDAVSSMLKLNYSRKHGQWEPNEYGGDGNLEAI
                     AFIKDLNETIFRDFPDVQTIAEEATDWPGVSKPTFEGGLGFGMKWMMGWMHDTLDYFK
                     FDPIFRQHHQNKFSFSMMYYYDENFMLPLSHDEVVHGKSPMLYKMPGDEWQKFANLRL
                     LYTYMWTHPGAKLLFMGSEFGQTSEWNYKSELQWELLQFDCHRLLKDCVSDLNKLLRQ
                     ESAMHFNQFSMDGFEWVDLNHRAESVIVYKRKGKKKGDDLLVILNMTPLVRQDWSIEV
                     IGKPYSAEIFNSDRTIYWGAGVVFNPEVRCELIDKAEKKYRVTVNLPALAGLILK"
     gene            2366..3427
                     /locus_tag="PLU11_12680"
     CDS             2366..3427
                     /locus_tag="PLU11_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN60031.1"
                     /translation="MLKIFITTCMLLSGSLVYSQTAVHTIVVPVDSADFFLQKGLTEK
                     QNGRRMESLKNFEKAVRYDSSSKSIITELASAYHDLRKYNNALVMYKRLIEMGEETPA
                     LYKQVLQLCFQMKQNEEVVKFASRLKKLDPSEKISFYVGKANYEMENYGDALKALNEA
                     AKEDPANAEIPYMIARSYADMMNYKLAVPFFKKAIELDPKQSYWVYELGLICYAMHDD
                     KNALKYILEAADKGHRRDNEYLENLAIAYLNVGQLDSGVAMLNEILKKRPSDMNILNM
                     VAEAFYYKAKYDQAMEYWDRVLEYDKENATALYMIGMCYQKKGGRENTDKGITLCDKA
                     IELDPSLANLKQKKMMAGL"
     gene            complement(3617..>3962)
                     /locus_tag="PLU11_12685"
     CDS             complement(3617..>3962)
                     /locus_tag="PLU11_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPN60032.1"
                     /translation="IQFIHARIKSILRKEQPAEAPYAGDLLKLEKELIVTLEQYPGVI
                     EQACAEHNPSLLAIYIFGIAKLYSSFYTEHHVLTAETAEKKQLRLKLSEMTAHVIASA
                     MGLLGIRVPERM"
BASE COUNT         1154 a          791 c          972 g         1045 t
ORIGIN      
        1 cggttatctg acctttaatg gtggaagact gggcataggt ggaaagggct ccggttagaa
       61 gcaaaaggcc caaaatgagc tttaatttca tggcttatgg ttggtttagg tcaggttaag
      121 gctttacagg ccgctaattt cctatgaaat tctgtttaat acaatgccgt tcgtgtaatt
      181 ttaagttaaa acacggaata accggaaata tatgagcgct aaaaagaata cgactgctgc
      241 tgataaagcc cgaaaaaaga aatctgttgc ttcgctgaaa gataaatcca ggcaaccggt
      301 tccatcatat agtaaaaaat atgaagaaga gaattttgta gataccagtt tactggtttg
      361 gaattactct ttacttactg atgaggatat tcagaattac cagcaaggca ccaactatca
      421 gttatataaa aaattcggtt ctcattccat tgaagtgaat aatacctggg gtatgtattt
      481 ctgtgtatgg gcgcccaatg ccaccgctgt ttccgtgaaa gggaatttca atgactggaa
      541 gaatcatgaa tatgcgcttt atccccgttg ggataaaagc ggggtatggg aagggtttat
      601 ccctggtttt aaactggggg aagcgtacaa gtatcatatt accggttttg ggaaaaaaga
      661 actggataaa ggggatccct ttgctaattt ctgggaaaag cggccacaga ccgccagtat
      721 tacctgggat atgcattatg actgggagga taaggagtgg atgaagaaaa gaaaaaaaca
      781 caattcactg gaggcgccct ggagtgtgta tgaactgcac ctggccagct ggatgcgccc
      841 tgacaagaat gatgaggaac gctataacag ttatgatgat atccgcgacc ggctggtgcc
      901 ttacctgaag gaaaccggct tcacccatgt ggagtttatg ccattaatgg aacatccgtt
      961 cgatggcagc tggggttatc agtgtaccgg cttctttgca gccacatccc gcttcggcga
     1021 tccgcaggga cttatgcggt tgattgatgc gcttcaccag gcagagatcg gggtgttgct
     1081 ggactgggtg ccttcgcatt tcccttacga tgcacacgga ttattcatgt ttgacgggac
     1141 tcacacttat gagtatgcgg atatgcggaa agggtttcat cccgactgga acagttatat
     1201 attcaactac aaaaggggag aagtgaagtc ctttctgatc agcagtgccc gtttctggtt
     1261 tgatcttttc catattgacg ggatacgggt ggatgcggtg tcttccatgc tcaaattgaa
     1321 ttattcacgt aaacacgggc agtgggagcc caatgaatac gggggagatg gcaacctgga
     1381 agccattgct tttattaaag acctgaatga aaccatcttc cgtgatttcc cggatgtgca
     1441 gacgattgct gaagaagcga cagactggcc gggtgtgagc aagccaacat ttgaaggggg
     1501 attgggtttt ggcatgaaat ggatgatggg ctggatgcac gataccctgg actatttcaa
     1561 atttgatccc attttccggc aacaccatca gaataagttt tcattcagca tgatgtatta
     1621 ctatgatgaa aacttcatgc tgcccctgag tcatgatgaa gtggtacatg gcaaaagccc
     1681 gatgctttac aaaatgcccg gtgatgaatg gcagaaattt gccaacctgc ggctccttta
     1741 cacgtatatg tggacccatc cgggcgccaa actgcttttt atgggcagtg aattcggcca
     1801 gacaagtgaa tggaattata aatcagaatt acagtgggaa ttgttacaat tcgattgtca
     1861 ccggctacta aaggattgtg tgagtgatct gaataaactg ctgcgccagg aatctgctat
     1921 gcattttaac cagttcagta tggatgggtt tgaatgggtt gacctgaatc accgggcgga
     1981 atcagtgatt gtgtataaac ggaagggcaa gaaaaaaggg gatgacctgc tcgttatcct
     2041 gaacatgacc ccattggtca ggcaggactg gagtattgaa gtaattggaa agccttattc
     2101 tgcagaaata tttaacagcg accgtacaat ttattggggg gctggtgtcg tttttaaccc
     2161 ggaagtaaga tgtgagctga tcgacaaagc agagaaaaaa taccgggtga ccgtaaattt
     2221 accggcactt gcgggtttaa tactgaagta attacgattt ggtactgaaa ttggttgata
     2281 aagaacggat taagctttga tttgtcattt ctgcgtttta tcttagttcc ccggctttca
     2341 cttatggaag gccaaaattc atcctatgtt aaaaatattc atcactacgt gtatgttgct
     2401 gtccggcagt ctggtttatt ctcagaccgc ggtacacacg attgttgtgc cggttgatag
     2461 tgccgatttc tttttacaaa aaggattaac ggaaaagcaa aatggccggc gaatggagtc
     2521 cctcaaaaat tttgagaagg cagtccgtta cgattcaagc agcaaatcca tcattactga
     2581 actggcatcc gcctatcatg atctgcggaa atacaacaat gccctggtca tgtacaaacg
     2641 cctgattgaa atgggcgaag aaacacctgc tctgtataaa caagtcttgc agctttgttt
     2701 ccagatgaag caaaatgaag aagtggtgaa atttgccagc cggctgaaaa aactggatcc
     2761 ttcggagaaa atcagtttct atgttggcaa agccaattac gaaatggaaa attacgggga
     2821 tgcactcaaa gctttaaatg aagcagcaaa agaagatccg gccaatgctg aaataccgta
     2881 tatgatcgcc cgcagttatg cggacatgat gaattacaaa ctggcggtgc ctttctttaa
     2941 gaaagcgatt gaactggatc ccaaacaaag ctattgggtc tatgaacttg ggctgatctg
     3001 ctatgcgatg catgatgaca aaaatgcgct gaagtatatc cttgaagcgg ccgataaagg
     3061 gcacagacgc gataacgaat acctggaaaa cctggccatt gcctacctga atgtgggaca
     3121 actggactcc ggtgtggcca tgctcaatga gatcctgaaa aaaaggccaa gtgacatgaa
     3181 tatcctgaac atggtggctg aagcgtttta ttacaaagcc aaatatgatc aggccatgga
     3241 atactgggat cgggtgctcg aatacgacaa ggaaaacgcc accgcccttt atatgatcgg
     3301 catgtgctac cagaaaaaag gcggacggga gaataccgat aaaggaatca ccctctgcga
     3361 taaagccatt gaactcgacc caagcctcgc aaatctgaaa cagaagaaga tgatggcggg
     3421 actgtagggg agtcggtagt catgagtcag tagtcggtag tcaacagccc atactgcgag
     3481 attattaaat aaaaaaatgc aaccacttta ttggttgcat tttttgttat actgaaaatc
     3541 tcatttattc ttagtataca atactcaggt ttcttctgaa aaatctgact cccgactacc
     3601 gactcatgac taccgactac attctctccg gtacccttat tcccagcaat cccatagccg
     3661 aagcgatcac atgagcggtc atttctgata attttaatcg caattgtttt ttctccgctg
     3721 tttccgccgt taatacatgg tgctcggtat aaaaggaact gtatagtttg gcaatgccaa
     3781 atatatagat agccagcaag gaggggttat gctccgcaca ggcctgttca ataacacccg
     3841 gatattgttc cagggtgacg atcagttcct tttcgagttt cagcagatcg cctgcataag
     3901 gcgcttcagc cggttgctct ttacgcagga tcgatttgat acgcgcatgg ataaactgga
     3961 ta
//
LOCUS       DAOPMW010000319         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27211_length_3947_cov_6.770298, whole genome shotgun sequence.
ACCESSION   DAOPMW010000319 DAOPMW010000000
VERSION     DAOPMW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27211_length_3947_cov_6.770298"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1213
                     /locus_tag="PLU11_12690"
     CDS             8..1213
                     /locus_tag="PLU11_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN60033.1"
                     /translation="MINSYLSLMKSFLFAALLISANLFAQDGRLSMGKLIERNFQLAA
                     SQYKLLEKNTPDSLMPRFFDPAANKAGTSNTKWWCSGFFPGSLLYIYEQTKDEEIRRI
                     AEKRLAILEKEKYYTGNHDLGFMIYCSFGNAYRLLGKPEYKEAVMTAAGSLASRYRPA
                     ISSIQSWNSSDRLKCPVIIDNMMNLELLYWVSDNGGDAKYRDIAVTHANTTLKYHFRP
                     DNSSYHVLDWDLEKKEIIKKITWQGAADSSAWARGQSWGLYGYTMMYRFSKDKAYLEQ
                     AKKIANYLLNHPNLPADKIPYWDFNAPGIPDTYRDASAGAVMASALLELGQYVSKKEK
                     KKYVGAAKQMLLSLSSNAYLTQPGENGGFLIKQNVGSLPHKSELKVSLTYADYYFLEA
                     MLRYKNWYL"
     gene            1220..1687
                     /gene="rnhA"
                     /locus_tag="PLU11_12695"
     CDS             1220..1687
                     /gene="rnhA"
                     /locus_tag="PLU11_12695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPN60034.1"
                     /translation="MSTTITMYTDGSSRGNPGPGGYGTILMSGTHRKELSQGYKRTTN
                     NRMELMAVIAGLKALKKDGMQVTVYSDSQYVVRAWEEGWLKNWIKTNFKGGKKNPDLW
                     REFYELSHKHHIKFKWVKGHADNPFNNRCDELATSAADAAHHTLLTDTGFEEK"
     gene            complement(2078..2650)
                     /locus_tag="PLU11_12700"
     CDS             complement(2078..2650)
                     /locus_tag="PLU11_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60035.1"
                     /translation="MKINGRLVLFTIALVAIATACKFYFGPNIEWSGFSPVIAIALFA
                     GFIIKQKDNSFLLPLLALFFSDVAIQVLYQQGLFDYPGFYGGQWKNYAVLLISTLIGW
                     GLKGRNYGSLLAGGLLAPTVFFLVSNLMVWSGAGAEAMYPKTFSGLMLCYEAGLPFFK
                     NSVMATLVFVPVVAMAYNLMTQKKAEIRLA"
     gene            complement(2709..3884)
                     /gene="manA"
                     /locus_tag="PLU11_12705"
     CDS             complement(2709..3884)
                     /gene="manA"
                     /locus_tag="PLU11_12705"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPN60036.1"
                     /translation="MKGVYTLKGVVKHYDWGGFSFIPGLLKQQNWEKKPFAEYWMGTH
                     PLGISTVEFEKGATGGLDILAGNLPYLFKAQEVKEMLSIQAHPSKKAAEIEFARENAE
                     GIPLDAAYRNYKDDNHKPEMLVALGDFWLLHGFKPEVQLESTLNDTAELQGLLPIWKE
                     GGYSALYKYIMEMPVEMVNKMLTPLVNRVLPLYEAGELQKDNPDFWAGRAVKTFTHDN
                     NYDRGIFSIYLFNIVHLSRGEGLFQDAGVPHAYLEGFNVELMANSDNVLRGGLTPKHI
                     DVPELLKHVKCEATIPAIIKGKAGEEGEINYATPVPDFRLSLFQLKAGETVAFTPESV
                     EIILLTDGIAELDDDENLVKLQLGNPSAVVFPGQVVYLAAATDCTVFRAREGIHNRE"
BASE COUNT         1112 a          988 c          860 g          987 t
ORIGIN      
        1 aaatctgatg atcaattctt atctttcact tatgaaatca ttcctttttg cagcgctgtt
       61 aatttccgca aaccttttcg cccaggacgg ccgtttgtca atgggaaaac tcattgagcg
      121 gaattttcag cttgccgctt ctcagtataa actccttgaa aaaaatacac ctgatagcct
      181 gatgccccgg ttctttgatc cggcagctaa taaagcagga accagtaata ccaaatggtg
      241 gtgcagcgga ttctttccgg gcagcctgct ctatatttat gaacagacaa aagatgaaga
      301 gatcagacgc atcgcggaaa aaagactggc catccttgaa aaagaaaaat actataccgg
      361 caatcatgat cttggcttta tgatctattg cagtttcggt aatgcgtatc gtctcctcgg
      421 caaacctgaa tataaagagg ccgtcatgac cgctgcgggt tccctggcca gccgttatcg
      481 tccggccatt agctccattc aatcctggaa cagcagcgac cggctgaaat gcccggtgat
      541 catcgacaat atgatgaacc tggaactact ttattgggta agtgataatg ggggtgacgc
      601 gaaataccgg gatatcgccg tgacccatgc gaacaccacc ctgaaatatc atttccgtcc
      661 ggataacagc agttaccatg tactggactg ggacctggaa aagaaagaaa ttattaaaaa
      721 aatcacctgg cagggggcag ccgattcatc agcctgggcc cgtggacaat cctggggact
      781 ctatggatac acgatgatgt accgtttctc caaagacaaa gcgtacctgg agcaggcaaa
      841 aaaaatcgct aactatttat tgaatcaccc caatttgccg gcggataaaa tcccctactg
      901 ggattttaat gcaccgggta ttcccgatac ttatcgtgat gcatcagccg gagcagtaat
      961 ggcatccgcc ttactcgaat taggtcaata tgtttctaaa aaagaaaaaa agaaatatgt
     1021 cggggctgca aagcaaatgc tcctctccct ttcctctaat gcgtacctga cccaaccggg
     1081 agaaaacggg ggattcctga tcaaacagaa tgtgggatct ttgccccaca aaagtgaatt
     1141 gaaggtatcg ctgacttacg ctgattatta tttcctggaa gcgatgctcc gatacaaaaa
     1201 ctggtacctg taaaataaga tgagcacaac gatcacgatg tatacagacg gatcctcgag
     1261 gggcaatccg ggtcccggcg gttacggcac cattctcatg agcggcaccc accgcaagga
     1321 actctcacag ggttacaaac gaaccaccaa taaccggatg gaactgatgg ccgtgatcgc
     1381 gggactgaag gccctgaaga aagacgggat gcaggttacg gtttattccg acagtcaata
     1441 cgtggttcgt gcctgggaag aaggctggtt gaaaaactgg atcaaaacca atttcaaagg
     1501 aggcaaaaaa aaccccgatc tctggcgtga attctatgaa ctctcccaca aacaccatat
     1561 aaaatttaaa tgggtgaaag gccatgccga caaccctttc aataaccgct gcgacgagtt
     1621 agccacttcg gcggctgatg cggcacatca tacgctgctc accgatactg gcttcgaaga
     1681 aaaatagaac tgcctgcggc aggatagttt gccactaaaa cacacaaaaa atcacgaaag
     1741 aatgttgctg cgcaacattt gggccacgga ttacacagat cagcacggat tgatgccctt
     1801 tgtatttttt gtgtcctttc gtgctgtttt gtggccaacc cttttttgcc gcaggcaaaa
     1861 aaatccgtgt tgatccgtgt gacctgcctg ccggcaaagg caggtccgtg gcttgtattc
     1921 ctgtattatt tcgtgccctt tcgtgtcaat tcgtggccac ctattcgccg ccaggcgaat
     1981 aaaaatccgt gttgatctgt gtgatccgtg gctacctttt tgccggaggc aaaaaaaatc
     2041 ccgcaccagc gggattaaat aagtttaggt ggcattctta cgccagtctg atctcggctt
     2101 tcttctgagt catcaggtta tatgccattg caaccaccgg cacaaacacc aacgtggcca
     2161 ttaccgaatt tttgaagaat ggcaaccctg cttcatagca aagcatcagc ccgctgaatg
     2221 ttttgggata catggcttcc gcaccggcac cgctccatac catcagatta gaaacgagga
     2281 aaaataccgt cggggccagt aaaccaccgg ccagcaaaga accatagttc cggcctttca
     2341 gtccccagcc gatcaatgtg gaaatcagca ataccgcata gttcttccat tgaccaccat
     2401 agaatcccgg gtaatcaaat aaaccctgtt gatataatac ctgaatggcc acatcactga
     2461 aaaacagggc cagtaagggt aaaagaaatg agttgtcttt ttgcttaatg ataaaaccgg
     2521 caaaaagcgc gatggcaatc accggggaaa aaccagacca ttcgatattg ggtccgaaat
     2581 aaaatttaca ggctgtggca atcgctacca gggctattgt gaaaaggact aaacgaccgt
     2641 tgatcttcat actgaatgat tataatgtga ggcaaaatta actgaattgg cccaaataga
     2701 ccacctagtt attcacgatt gtggataccc tcccgggccc tgaaaaccgt gcaatcggta
     2761 gccgccgcca gataaacaac ctgtcccgga aaaaccacgg ctgaggggtt ccccaattgc
     2821 aatttcacca ggttttcgtc atcatccagt tccgcaatgc catcagtcaa aaggatgatt
     2881 tccacagatt ccggggtaaa agccaccgtt tcccccgctt tcagctgaaa cagactaagt
     2941 ctgaaatccg ggactggtgt tgcataattg atttccccct cctcacctgc ttttcctttt
     3001 ataatagccg gaatggtagc ttcgcatttc acatgtttga gcaactccgg cacatcaata
     3061 tgtttggggg ttaatccgcc ccttaacaca ttatccgaat tagccataag ctctacatta
     3121 aacccttcca ggtaagcatg gggcaccccc gcatcctgga aaagtccctc tccgcgactc
     3181 aggtgcacta tattgaacag gtaaatggag aaaatacccc ggtcataatt attgtcatgg
     3241 gtaaaggtct tcactgcccg gcctgcccag aaatcaggat tatctttttg caattccccg
     3301 gcttcataca acggaagcac cctgtttacc aggggcgtaa gcattttgtt taccatttct
     3361 accggcattt ccatgatata cttatacaaa gcgctgtatc cgccctcttt ccagatggga
     3421 agtagcccct gaagttctgc tgtatcattt aatgtggact ccagctgtac ttccggctta
     3481 aacccatgca acaaccagaa atctcccagt gctaccagca tctctggttt atgattatcg
     3541 tctttataat tacggtatgc ggcatccagc ggaattcctt ctgcattttc gcgggcaaat
     3601 tcaatttccg ctgctttttt cgaaggatgt gcctggatag acaacatctc cttcacttcc
     3661 tgggccttga ataaataagg cagattaccg gccagaatgt caagtccgcc ggtagctcct
     3721 ttttcaaact ccacggtgga aatgcccagc ggatgtgtcc ccatccaata ctcagcaaac
     3781 ggtttttttt cccaattttg ctgttttaaa agcccgggga taaaggaaaa tcctccccaa
     3841 tcatagtgtt taacaacgcc tttcaaggtg taaactccct tcatagaaaa tttgtgttgg
     3901 tttaacttac tgtaaagata agttatggca gcgcacaacg aactcgg
//
LOCUS       DAOPMW010000320         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27374_length_3930_cov_8.013419, whole genome shotgun sequence.
ACCESSION   DAOPMW010000320 DAOPMW010000000
VERSION     DAOPMW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27374_length_3930_cov_8.013419"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..977)
                     /locus_tag="PLU11_12710"
     CDS             complement(21..977)
                     /locus_tag="PLU11_12710"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPN60037.1"
                     /translation="MRFEQPVPLTTIATMIGATLIGNKEGMATGINEIHKVETGDLVF
                     VDHPKYYATCINSAASFIIINTETDIPEGKALLVVSDPFEAYLKIVQFYRPFTPSGKA
                     ISDSATIGEGSVVMPGAFIGNHVQIGRNCIIHPNVSILDHCILGDEVIIQAGTVIGSD
                     AFYYNKKTNRDIHYKRMASCGRVILENAVEIGANCTIDRGVTHDTILGAGTKLDNLVH
                     VGHDTTVGKNCLIAAGVGIAGAVIIEDNVILWGQVGVNKTLTIGQGAIVLGQTGVTNS
                     IEGGKTYFGTPMEDARIKRRELVWVKRIPELWEKVMGQKDKD"
     gene            1582..2457
                     /locus_tag="PLU11_12715"
     CDS             1582..2457
                     /locus_tag="PLU11_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPN60038.1"
                     /translation="MLKSMTGFGRAEKNVADKTFLVDIKSLNGKQFELQLKIPAFLKP
                     FEFDIRRLLSEKLGRGSVDCIISLKDTGTAKPVTINTDLAKAYYQPLAELSKALNLDA
                     SNILSTLVKLPEVITPSSDTLTDAEWNQFQSVLETAIGNLNTHRLDEGKSLEDDLTAR
                     INNIGQLQEEVIKLEPLRKQKIREGITRLLEENVGKENYDGNRLEQELIYYIEKIDIS
                     EEQVRLKNHLDYFKDLLADPDDAKGKKLSFILQEIGREINTTGSKAYDSTIQKCVVQM
                     KDELEKAKEQVLNVL"
     gene            2495..2977
                     /locus_tag="PLU11_12720"
     CDS             2495..2977
                     /locus_tag="PLU11_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPN60039.1"
                     /translation="MKLLQPFAFIVLVLGTFSCTHTDLYEKTAVIPEHSWKSTFKPSF
                     SFTIKDTSSPYMVYLIIRHNDRYSFNNIYVNLYAKGPGQDTAQVVPYDMRLGTDETGW
                     LGSGMDDIYEHREPITPRSSEKAFYFRKPGDYTFTIEQLMRQDPLENVLNAGIRIEKK
                     "
     gene            2987..>3930
                     /locus_tag="PLU11_12725"
     CDS             2987..>3930
                     /locus_tag="PLU11_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60040.1"
                     /translation="MPDETRKRLKRTTVIYCTLLFYVLAALIWWFISLEKQSKEIARQ
                     QYENLAARSETMSTLKLADEMQAITREEKRNTRKYIAEGITFLILIVIGALMVYRSIR
                     KQFKLQQQQQNFMMAVTHELKTPIAVARLNLETLQKYTLDPEKQKKLIRNTLDETTRL
                     HLLTNNILVSSQLESDRYHSAMEELDLSTLLKDCMRDFRKRFPDRVFEEEIEEEADLR
                     GDPLLLQMLINNLVENAIKYSPKEGKIKAVLAKASGSIRLQVIDEGPGIPEEEKTRVF
                     SKFYRIGNESTRKAQGTGLGLYLCRKIARDHNGDIAVT"
BASE COUNT         1111 a          961 c          845 g         1013 t
ORIGIN      
        1 tgattaattt attcttaaga tcagtctttg tctttctggc ccatcacttt ttcccagagc
       61 tcgggaatcc gtttcaccca taccagttct ctgcgtttaa tacgggcatc ctccattggt
      121 gtgccgaaat atgtcttgcc cccttcgatg ctgttggtca cacctgtctg tcccaacaca
      181 atggcccctt gtccgatcgt cagtgtttta ttgacgccca cctgtcccca aagaatgaca
      241 ttgtcttcaa taataaccgc accggcaata cccaccccgg cagcaatcag gcaattctta
      301 cccaccgtgg tatcatgtcc tacatggacc aggttatcca gtttggtacc tgcaccaaga
      361 atggtatcat gcgttacccc gcggtctatc gtacaattgg caccaatctc cacggcattt
      421 tccaggatca ccctgccgca actggccatc cgtttgtagt ggatatcccg gttggttttt
      481 ttattgtaat aaaaggcgtc actgccaatc acggtaccag cctgtatgat cacctcatca
      541 ccgaggatgc aatgatcgag gatacttaca ttgggatgaa tgatgcagtt cctgccgatt
      601 tgtacgtgat taccaataaa tgctccgggc atcaccacgc ttccttcgcc gatagtggct
      661 gaatcactga tggccttccc ggatggcgtg aagggccggt agaactgtac gatcttcagg
      721 tacgcttcaa acggatcact aactaccagc agggccttcc cttccggtat atccgtttca
      781 gtattgataa taataaaact ggcggcagag tttatacagg tggcataata cttggggtga
      841 tcgacaaaga ccaggtcacc agtttcgact ttgtgtatct cattgatccc ggtggccatt
      901 ccctctttat tgccaatgag ggtggcacca atcatcgtgg caatcgtagt aagaggaacc
      961 ggttgttcaa atcgcatatc attcgttttg ccaaaggtaa taagggaatc attggcgaaa
     1021 agaccctttt tgcaaccgga ggcagcggat accggtttcc gcattcacgt gtctttacgc
     1081 tactgtcttc tccgctttat tcagttgcat ttattcaatc attgttgttg catttatgat
     1141 ctgttgttgt tgcataaaaa aaacaaaacg cctgaaaccc gctgttcatg catgtttcag
     1201 acgatctttt tttttattcg ctgataaaaa aaatatcggc actattgaaa tgcagcaaac
     1261 aaacagatca gttattgatg aaaaattatt tggtttatcc acagctgatt ttaaaatgtt
     1321 aataaaatag tttggaaata tttttttgtt acataaaatt atttttaata ttgcataggg
     1381 aatgaaagtt ccctgtactt actaacccat ccatatatta tccctccgct tactacggag
     1441 ggattttttt tagctttgta tcaataaaaa acagacttct tatacttttc cggttcaatc
     1501 attatctctg ctcaccgttt tttacccggc tgattaatca ataataaaaa atacctttac
     1561 aggaatcata aatcccgttc catgcttaaa tcaatgaccg gttttggaag agcagaaaag
     1621 aatgtagctg acaagacctt cctggtggat atcaaatcgc tcaatggcaa acaatttgaa
     1681 ctgcagttga aaatccccgc tttcctgaag ccctttgaat ttgacatccg tcgcctgtta
     1741 tcggaaaaac tcggcagggg cagtgtggat tgtatcatca gtctgaaaga taccggcacc
     1801 gccaaacccg ttaccatcaa taccgacctg gccaaagctt attaccagcc cctggccgaa
     1861 ttatcgaaag ccctgaacct ggatgcttcg aatatcctga gcaccctggt taaattaccg
     1921 gaagtaatca cgcccagcag cgataccctc accgatgccg aatggaatca gtttcagtcc
     1981 gtactggaaa ccgccatcgg caacctgaac acacatcgcc tggatgaagg caaatccctt
     2041 gaagatgacc tgaccgcccg tattaataat atcggccagc tacaggaaga agtgatcaaa
     2101 ctggaacccc tccgcaaaca aaagatccgg gaaggtatta cccgcctgct ggaagaaaat
     2161 gtgggcaagg aaaactatga tggcaaccgc ctggagcagg aactcattta ttatattgaa
     2221 aagatcgata tcagtgagga acaggtgcgg ttgaaaaatc acctggacta tttcaaagac
     2281 ctcctggctg acccggatga tgcgaaaggg aagaaactct cttttatcct ccaggaaatc
     2341 ggccgggaaa tcaataccac cggttccaaa gcctatgact ccaccatcca gaaatgtgtg
     2401 gttcagatga aagacgaact ggaaaaagca aaagaacagg tattgaatgt actctgagcg
     2461 ttgcgttctg tttatttttg taaaaaccgt ttacttgaaa ctccttcagc cctttgcctt
     2521 tattgtcctc gtgctcggaa ccttttcctg cacccatact gatttgtatg aaaaaacagc
     2581 cgtgattccg gaacatagct ggaaaagtac atttaaacct tcattcagtt ttaccataaa
     2641 ggacaccagt tcgccttata tggtatacct gatcatccgg cacaatgacc gctatagttt
     2701 caataatatt tatgtgaacc tgtatgctaa aggacccgga caggatactg cacaggtggt
     2761 tccttacgat atgcgtttgg gaaccgacga aacgggctgg ctcggcagtg gcatggatga
     2821 tatttatgaa caccgggaac ccatcacacc ccggtcatct gaaaaagcgt tttatttccg
     2881 taaacccgga gactatacgt tcacgattga acaactcatg cgccaggacc cactggaaaa
     2941 tgtcctgaat gccggaatac ggatcgaaaa gaaataacag cttcccatgc cggatgagac
     3001 acgaaaaaga ctgaaacgca ctacggttat ttactgcacc ctgcttttct atgtactggc
     3061 cgcactgatc tggtggttta tctccctcga gaaacaaagc aaggaaattg cccggcagca
     3121 atatgaaaac ctggcagccc gctcggaaac catgagcacc ctgaaactgg cggacgaaat
     3181 gcaagccatt acccgtgagg aaaaacggaa tacccggaaa tatatcgcgg aaggtatcac
     3241 cttcctgatt ctgatagtga ttggtgccct gatggtttac cgctcgatcc gtaaacaatt
     3301 caaactgcag cagcaacagc agaattttat gatggccgtc actcatgaac tcaaaacgcc
     3361 cattgccgta gcgcggctca acctggaaac cttacagaaa tacacattgg atccggaaaa
     3421 gcagaaaaaa ctgatccgga atacgcttga cgaaaccacc cgcctgcacc tgctgaccaa
     3481 taatatcctg gtctcttccc agctggaatc cgaccggtat cactccgcca tggaagaact
     3541 ggacctgtct acactgctga aagactgcat gcgtgatttc aggaaacggt tcccggaccg
     3601 cgtgtttgag gaagagattg aagaagaggc cgatctccgg ggtgatccct tgttattaca
     3661 aatgctgatc aacaacctgg tggagaacgc catcaaatat tcccccaaag aaggaaagat
     3721 caaagcggtg ctggccaaag cgtccggatc gatccggctg caggttattg atgaagggcc
     3781 gggtatacca gaagaggaaa agaccagggt tttctccaag ttttaccgga tcggcaatga
     3841 atctacccgg aaagcgcagg gaaccggact gggattgtac ctttgccgta agattgcccg
     3901 cgaccataat ggcgacattg cggtgacaaa
//
LOCUS       DAOPMW010000321         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27535_length_3914_cov_12.498316, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000321 DAOPMW010000000
VERSION     DAOPMW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27535_length_3914_cov_12.498316"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLU11_12730"
     CDS             <1..468
                     /locus_tag="PLU11_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPN60041.1"
                     /translation="AKQHIDRFAADHDEYNKILVQILADRMVEAFAECLHEQTRKEFW
                     GYDKEEQLSNVQLIHEEYKGIRPAPGYPACPDHTEKIKLFELLNVTENIGITLTESLA
                     MNPPASVCGWYIGHPKSHYFGLGKISKDQLVDYAKRKGMPLEEAERWLRPVLE"
     gene            complement(1289..2236)
                     /locus_tag="PLU11_12735"
     CDS             complement(1289..2236)
                     /locus_tag="PLU11_12735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HPN60042.1"
                     /translation="MSPASDFTAFYEKELRELLLPLEDQRKKIKKWGVYGFVLLGLAI
                     VFFIIGTTSQLPAAIIIAIVVFVAAIIVLILFANRLKKFTGIFKTQIVSRIVAFINPS
                     LSYSPERFIAEADYKKSGLYLKSSDRYKGDDYVEGLHDKTFFCFSELHTEERVSSGKN
                     TRWETIFKGMFIIADFNKNFSGRTYVWSENKPQLNFLNKLFSSFASGLEKVKLESPDF
                     EKRFIVYSNDQVEARYILTPSFMERLVRLQQMTSTDTSMSFVQTNIHIAIPMNKELFE
                     PSVFKANDFSRLEQFRSTIQIVYEIIDELKLNERLWTKE"
     gene            complement(2246..2794)
                     /locus_tag="PLU11_12740"
     CDS             complement(2246..2794)
                     /locus_tag="PLU11_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPN60043.1"
                     /translation="MYIAIGVLVVLLLVIIGLYNSLIVKKNAVDNAFFSMDVMLKKRH
                     DLIPQLLDTVKGYMQHEKELLNNLTRLRQQMDLPQLPVNEKIKLENQLQSILQQVRVQ
                     VENYPDLKASANFLKLQGAINETEEQLAASRRFYNAAVNDYHNGIQTFPSSIIASMAG
                     MVRKEYFAVGEDAKEVPVVNFK"
     gene            complement(2804..3415)
                     /locus_tag="PLU11_12745"
     CDS             complement(2804..3415)
                     /locus_tag="PLU11_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPN60044.1"
                     /translation="MAVNTFIWDLGGVLVDWNPHYVFHEHYFESAEKRNYFFEHICTH
                     DWNEQQDAGRSIVEATQELVARFPEWEREIRDYYGRWTDMLNGPVHGTVSIFRELIDS
                     GRYKHYALTNWQADLFNIALVRYDFLHWFDGRVVSGVEKVRKPFPAFYQRLLDRYQVA
                     PANTVFIDDNLRNVKAAEEMGIKSIHFHHPDQLRAELQQLSLI"
     gene            complement(3419..3679)
                     /locus_tag="PLU11_12750"
     CDS             complement(3419..3679)
                     /locus_tag="PLU11_12750"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HPN60045.1"
                     /translation="MTAADILGYAAGAVTAFTFLPQVVKTWKEKSAKDISLYMFLIAF
                     VNEVMWLVYGFMIDNMVVILTNAVMLVMSGIMIGLKLKYNHK"
     gene            complement(3679..>3914)
                     /locus_tag="PLU11_12755"
     CDS             complement(3679..>3914)
                     /locus_tag="PLU11_12755"
                     /inference="COORDINATES: protein motif:HMM:NF025862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HPN60046.1"
                     /translation="NFTQSLSFLDADKKYVATIYRDADNAHWKDNPEAYVIEKFLVDA
                     KTMLKLKLVPGGGTAVSLIPAKAEEVKLYKKYK"
BASE COUNT         1089 a          932 c          854 g         1039 t
ORIGIN      
        1 gccaaacaac atatcgaccg ttttgcggcc gatcatgatg aatacaataa aatattggtg
       61 cagatcctgg cggaccggat ggtggaagct tttgcagagt gcctgcatga acagacccgg
      121 aaggaattct ggggctatga taaagaagag caactcagta atgtgcagtt gatccatgaa
      181 gaatacaagg gtattcgtcc ggcaccgggt taccccgcct gcccggatca taccgagaaa
      241 attaaattgt ttgagctgtt gaatgttaca gagaacattg gtattacgct gacggaaagc
      301 ctggcgatga atccgccggc gtcagtttgc ggctggtata tcgggcatcc gaagagccat
      361 tatttcggat taggcaagat aagtaaagat cagttggtgg actatgccaa acggaaaggc
      421 atgccgctgg aggaagcgga aagatggttg cgccctgttt tagaataaaa aatgaaaaat
      481 caagatatac cggttatcct gaaaagggta accggttttt tattgggaat atatcggtgc
      541 aatacaccgg gcttcgccct tgtgtaattt cattcaaccc cgttggggtt gcgggggtcg
      601 tcgcaatgga caaaccccgg gttgcacccg gggctatgaa aagttcaacc ccgttggggt
      661 taggagtggt atcaccggat acgggcaatt ttatttccat gaagttctga atattaaatt
      721 atgagaaaca aacaatcagt tgaaattgta cacccgatca aatgccaatt gattccctac
      781 aatccattta caaatccttt gtgtaacgcc atacgtcagt ggtacaaaac accgggcttc
      841 gcccttgtgt aatattcatt caacccctcc agggttgcgg ggatcatcca aatggccgaa
      901 ccccgggttg cacccggggc tatcaaaagt tcaaccccgt tggggttggc agtgatatta
      961 tcggaattgg actatttaag ttccatgaat tgctgaatat taaaattccg gtcacctggc
     1021 aatcggttcc tccaattaaa atgaaatccc ggattgaatg actgaatagc ccgaagggct
     1081 taaattatta atagccccgg gtgcaacccg gggtacggga ccaacgtgat gcccgcaacc
     1141 ccgacttgtc cgccgtagca aagcgaaggt ggaacggggt tgaactatct ggatggcatt
     1201 aacataacaa aataccctaa cactcaccta ttgtccaatg cattgaactg ccaactgctg
     1261 cctgcctact gcctactgcc gactgcccct actccttcgt ccacaacctt tcattcaatt
     1321 tcaactcatc aataatttca taaacaatct gaatggtgga ccggaactgc tccagccggc
     1381 tgaaatcatt ggccttgaaa accgatggct cgaaaagttc tttattcatg ggaatggcaa
     1441 tatgaatatt tgtctgaaca aacgacatgg aagtatccgt actggtcatt tgctgcagtc
     1501 gtaccagtcg ctccataaaa gagggcgtga ggatataacg ggcttccacc tggtcattgc
     1561 tatacacaat aaaccgcttt tcaaaatcag ggctttcgag ttttactttt tcaagtccgg
     1621 aagcgaagga agaaaacagt ttgttcagga aattaagttg tggtttgttt tcactccata
     1681 catacgtgcg gccggagaaa tttttattaa aatccgcgat gataaacatc cctttgaaga
     1741 ttgtctccca acgggtgttc ttcccagaac tgaccctttc ttccgtatgc agctcggaaa
     1801 aacaaaaaaa ggttttatcg tggagacctt ccacataatc atcgccttta tagcggtcag
     1861 aacttttcag gtaaagtccg ctctttttat aatctgcctc agcaataaac cgttccgggc
     1921 tataactgag cgacggattg ataaaggcaa caatacggct aacgatctgt gttttaaaaa
     1981 taccggtaaa tttctttagc cggttcgcaa acaggatcag cacgataatg gctgccacga
     2041 aaacaactat ggcaataatg atagcagctg gcagctgact ggtggttcct ataataaaaa
     2101 aaacaatggc gagtcccagt aaaacaaagc cgtatacgcc ccacttttta attttctttc
     2161 gttgatcttc cagcggaagc agcaactccc tcagctcctt ttcataaaat gcggtaaaat
     2221 cactggcagg agacataggt gaattttatt taaagttgac caccggtact tctttcgcgt
     2281 cctcacccac ggcaaaatat tctttacgga ccatgcccgc cattgaagca atgatagagg
     2341 aaggaaaagt ctggatgcca ttatggtaat cattcaccgc ggcattatag aaccggcggg
     2401 aagcggccag ttgctcttcg gtttcattga tcgccccttg aagcttcaga aaattggcac
     2461 tggctttcag atccgggtag ttttccacct gcaccctgac ctgttgtaag atggactgaa
     2521 gctgattttc cagtttgatt ttctcattca ccggcaattg gggcaggtcc atttgctgcc
     2581 gtaaacgggt caggttgttg agcaattctt tttcatgctg catatatccc ttaaccgtat
     2641 ccaggagctg cgggatcagg tcatgccttt ttttaagcat gacatccatg gagaaaaaag
     2701 cattgtctac tgcgttcttc ttcacaataa gactgttata caggccaatg atgaccagta
     2761 acagcacaac caggacgcca atcgctatat acatatttac cagttaaatc agggacaatt
     2821 gctgcagttc agcccgcagc tgatcaggat ggtggaaatg aatactctta atacccatct
     2881 cctccgctgc tttcacattc cgtaaattat catctataaa tacagtgttt gccggcgcca
     2941 cctggtagcg atccagcaaa cgctgataaa atgcaggaaa gggtttgcgg actttctcca
     3001 cgccactcac taccctgccg tcaaaccaat gcaggaaatc atagcgtacc agcgcgatat
     3061 tgaacagatc cgcctgccag ttggtcagcg cgtaatgttt gtatctacca ctatcgatca
     3121 gttcacggaa aatggatacc gttccatgta ccggaccatt cagcatatcg gtccagcggc
     3181 cataataatc ccggatctcc ctttcccatt cgggaaaccg ggccaccagt tcctgcgtgg
     3241 cttccacaat ggaacggccc gcatcctgct gctcgttcca gtcatgggtg caaatatgtt
     3301 caaagaaata atttcttttt tcagccgatt caaaatagtg ctcgtgaaat acatagtggg
     3361 ggttccagtc cactaaaact ccgcccaaat cccagataaa tgtatttacc gccatagact
     3421 acttatggtt atattttaat ttcagaccga tcatgatgcc ggacatgacc aacatcacgg
     3481 cattggtcag gatgaccacc atattatcaa tcataaaacc gtataccagc cacatgacct
     3541 catttacaaa ggcgatgaga aacatataaa gtgaaatatc tttggcagac ttctctttcc
     3601 aggttttcac cacctgtggc aaaaaggtaa aggcggttac ggctcctgcg gcatagccca
     3661 gtatatcggc tgctgtcatt atttatactt tttatacagt ttgacttctt cggcttttgc
     3721 cggtatcaaa cttacggcag ttccgccacc cggtaccaat tttaatttca acatcgtttt
     3781 ggcatccacc aggaactttt cgatcacata cgcttccgga ttatccttcc aatgggcatt
     3841 gtcagcatcg cggtagatcg tggccacata ttttttatcc gcatccagga aggacaatga
     3901 ctgtgtgaaa ttcc
//
LOCUS       DAOPMW010000322         3900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27681_length_3900_cov_5.298049, whole genome shotgun sequence.
ACCESSION   DAOPMW010000322 DAOPMW010000000
VERSION     DAOPMW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27681_length_3900_cov_5.298049"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLU11_12760"
     CDS             complement(<1..454)
                     /locus_tag="PLU11_12760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN60047.1"
                     /translation="MKKIFFLLALLSMQYGYAQKKTTRPVDPLAGIDTLLERILKDRK
                     AAGFAVAVVKKDKIIYAKGFGYRDYEKKMPVTPNTLFAIGSCTKAFTSSLMGLLNKDS
                     KLEYDKPARDYLPALQFYNNELNNGVTVRDIMCHRTGLPRHDFSWYLFN"
     gene            complement(471..1712)
                     /locus_tag="PLU11_12765"
     CDS             complement(471..1712)
                     /locus_tag="PLU11_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPN60048.1"
                     /translation="MEKNFPTDSLNNQAPFTGWSSVAVHGGHKKDPMYAHQVPIYASS
                     TYVFDSAEQGMRRFDGKEEGYIYSRWGNPTMTEAEEKITALETFQLGMEAKGILHASG
                     MAAITTLLLANLKPGDKILSHYSLYGGTNEIISKVLPGLQINPVIADLRNLNLAEELM
                     KADPQIKMLYLETPANPTIQCVDLEALCGLAKKYNIITACDNTFATPYLQQPFKQGVD
                     FIVHSTTKFLNGHGTAIGGILLGRDLAMMNGPVTKLHRTLGGNSNPFDAFLLIQGMKT
                     LEVRMERHCHNAMEVAGFLENHPAIARVNYTGLASHPDHTVAMKQMRHPGAMLSFELK
                     GGLQAGIGFMNRLQMCVRTVSLGTCDTLLSHPASMTHYSVPKAEKEKYGITDGLIRMN
                     TGIENIQDIISDLDQALQPFK"
     gene            1813..2307
                     /locus_tag="PLU11_12770"
     CDS             1813..2307
                     /locus_tag="PLU11_12770"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPN60049.1"
                     /translation="MKELMKQQAAHNHWANQRMADTLLLLPDGVCIQEVSSSFNSLQK
                     TLLHIWDAESGWWQRLKLQERIELPGKTFNGRIEEAAGGLLYQSRQWEDWTKEASANA
                     LDHVIQFQTSKRELVKIKTCQLLLHVFNHSTYHRGQLITMLYQLGYPKVPSTDFFLWL
                     KMKK"
     gene            complement(2310..2543)
                     /locus_tag="PLU11_12775"
     CDS             complement(2310..2543)
                     /locus_tag="PLU11_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60050.1"
                     /translation="MSSRSRANKKFWNKVFRLTLIYLLIVPVIFYLLDKSSVSNDLKN
                     TPVGFILKMAGIAFGISLIISFWGHRDPELRKY"
     gene            complement(2649..3656)
                     /locus_tag="PLU11_12780"
     CDS             complement(2649..3656)
                     /locus_tag="PLU11_12780"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPN60051.1"
                     /translation="MSKKITATITAVGGYVPEDKLTNFDLEKMVDTNDEWIRTRTGIS
                     ERRILRGEGKATSDMVVPAVQELCRKRGISPEEIDCLIVGTVTPDMVFPSTANIACDK
                     LGTKNAWGFDVAAACSGFLYSLTQGAALIESGRYKKVVVCGADKMSAIIDYSDRATCI
                     IFGDGAGAVLLEPSEEEGYGILDSILRSDGSGAPFLHMKAGGSLRPASMATVMAKEHY
                     AYQEGQAVFKFAVKGMADVSAELLERNNLTGEDIAWLVPHQANLRIIDATANRMGLPR
                     EKVMINIERYGNTTAGTIPLCLWEWESQLKKGDNVILSAFGGGFTWGATWVKWGYNGT
                     K"
BASE COUNT          976 a          916 c          912 g         1096 t
ORIGIN      
        1 tattgaaaag ataccaggag aaatcatggc ggggtaatcc ggtacggtgg cacatgatat
       61 cccttaccgt aacgccatta ttcaactcgt tgttatagaa ttgcagagca ggcaggtaat
      121 ccctggcggg tttatcgtac tccagtttgc tgtccttatt tagtaaaccc ataagggagg
      181 aggtaaatgc cttggtacag gagccaatgg cgaatagtgt attgggcgtg accggcattt
      241 tcttttcata atcccggtaa ccgaaccctt tcgcataaat gatcttatcc ttcttcacca
      301 cagccactgc aaatcccgct gccttccggt ctttcagtat tcgttccagc agggtgtcaa
      361 tcccggcgag gggatcaaca gggcgtgttg ttttcttttg agcatagcca tattgcatac
      421 tgagcagggc cagcaggaag aagatttttt tcataacagt ttgttttagc ttatttgaat
      481 ggttgcaatg cctgatcgag atccgaaata atgtcctgga tattttcaat cccggtattc
      541 atccggatca gcccatcagt gatgccgtat ttttcttttt cggctttagg tacactatag
      601 tgagtcattg aggctggatg agacaataac gtgtcgcagg tgcccaatga tacggtacgc
      661 acacacatct gcagccggtt cataaatccg ataccggcct gcagcccccc tttgagttca
      721 aaactcagca tagctcccgg gtgccgcatt tgtttcatcg ccacggtatg atcgggatga
      781 ctggccaggc cggtataatt tacccgggct atcgccggat gattttccag gaagcccgcc
      841 acttccatag cattgtggca atgtctttcc atccgcactt ccagggtttt cattccttgt
      901 ataagcagga aagcatcaaa tgggttggag ttgccaccca gggtccggtg cagtttagtg
      961 accgggccat tcatcattgc aagatcccgc cctaaaagta ttccaccaat agcagtacca
     1021 tgtccgttga gaaacttggt cgtggagtgt acaataaaat caacaccctg tttaaaaggt
     1081 tgctggagat agggagtggc aaacgtattg tcgcaggcgg tgatgatatt atattttttc
     1141 gccaatccgc atagtgcttc caggtccaca cattggatcg tgggattggc cggcgtttcc
     1201 aggtataaca tttttatctg cggatcagct ttcatcagct cttctgccag gttcaggttg
     1261 cgcaggtcag caatcaccgg gttgatttgt aatccgggca gaactttact gatgatttca
     1321 ttggtgccgc cgtataatga gtaatgacta agtattttat cgcccggctt caggttggcc
     1381 agcagcaggg tggtgatggc ggccattccg gatgcatgaa gaatgccctt cgcttccata
     1441 cccagttgaa aggtttcaag tgccgtgatc ttttcttcgg cttccgtcat agtgggattg
     1501 ccccagcggc tgtaaatata tccttcttct tttccgtcga aacggcgcat accctgttcg
     1561 gcggaatcga atacataggt actgctggca tagataggca cctggtgggc atacatcgga
     1621 tcttttttat gtccgccatg tacggccacg gaactccagc cggtaaatgg tgcctgatta
     1681 ttcaaagaat cggtgggaaa gtttttctcc atgtttatga attgtgtccg aattttatcg
     1741 gaaccggtaa tcgagaaagc aataaaagcc ttctcaaatt tagcggtttt caatctttaa
     1801 tgagcagcaa ctatgaaaga actgatgaaa cagcaggccg cccataatca ctgggcaaat
     1861 caacggatgg cggacacact cttgttgctg ccggatgggg tttgtataca ggaagtttct
     1921 tccagtttca acagtcttca gaaaaccctg ctgcatatct gggatgcgga atcgggctgg
     1981 tggcagcggt taaaactgca ggagcggatt gagctgccag gtaaaacgtt taatggcagg
     2041 attgaggagg ccgctggcgg tttgctatat cagtccaggc aatgggaaga ttggacgaaa
     2101 gaagcttcgg caaatgccct ggaccatgtg atccagtttc aaaccagcaa gcgggaactg
     2161 gtgaaaataa aaacctgcca gctgttgctg catgtattca atcacagcac gtatcaccgg
     2221 ggacaactga tcaccatgct gtatcagctg ggttatccga aagtcccttc aacggatttc
     2281 tttctttggc taaaaatgaa aaagtaagat cagtatttgc gtaattcggg atcgcgatgt
     2341 ccccagaagc taatgatcag cgaaatgccg aatgcaatcc cggccatttt taatataaag
     2401 ccgaccggtg tatttttcag gtcattactg acactggatt tgtccagcag gtaaaaaatt
     2461 acaggtacaa tcaggagata gatcagggta agtcgaaata ctttgttcca gaattttttg
     2521 ttggcacggc tacggctaga cataatcagt tctacaatca gattttaaat aagagactcc
     2581 ttaacgggcg caagataggg agaaaccact tctcactgcc gttaatgttt aatgataaca
     2641 atcaattatt acttcgttcc gttatatccc catttcaccc aggtagctcc ccaggtaaaa
     2701 ccaccaccaa aggctgacag tatcacattg tcgccttttt ttaactggct ttcccattcc
     2761 cataaacaaa gggggatggt gccggcggtt gtattaccat accgttcgat attgatcatc
     2821 actttttccc tgggaagtcc catccggttg gccgtggcat caatgatccg caggttggcc
     2881 tggtgtggaa ccagccaggc aatatcctca ccggtcaggt tatttctttc cagtaattcg
     2941 gcactcacgt ccgccatacc ttttacggca aatttgaaaa cggcttgacc ttcctggtaa
     3001 gcataatgtt ctttagccat gaccgttgcc atactggccg gacgtaatga tccaccggct
     3061 ttcatatgca ggaaaggggc gccgctgcca tctgagcgga ggatactgtc gagtatgcca
     3121 tagccctctt cttccgaagg ttcaagcagt acggcaccgg ctccgtcgcc aaaaataata
     3181 caggtggcac ggtcactata gtcgatgatc gcactcattt tatcggcccc gcaaaccacc
     3241 acttttttat accgaccgct ttcaattaat gcggcaccct gggtcagtga atataaaaaa
     3301 ccggaacaag cggcagccac atcaaatccc caggcatttt tggtacccag tttatcgcag
     3361 gcaatattgg cagtagaagg gaataccatg tccggggtta ctgttcccac aatcaggcaa
     3421 tcaatttctt ccggactgat tcctcttttg cggcataatt cctgcacagc cggcaccacc
     3481 atgtcggatg tggctttgcc ttcccctctc agtatccttc tttcagaaat gccggtgcgg
     3541 gtacggatcc actcatcatt ggtatccacc attttctcca gatcaaaatt ggtcagtttg
     3601 tcttccggta catagccccc cactgcggta atggtggcgg tgattttttt actcatgcct
     3661 tgtgaattag taaagtttgc aaatttaagg aaaatgagag aagtcgttag tcgttagtca
     3721 atagtcctta gtcaacagcc cattctaagt aaatttgttg gtatttaagt aattcttgtt
     3781 caaccagaac atttacaaag caagcaccaa acatccttag aaggattggt gcttatttga
     3841 tatttggttc ttggttcttc tttggcttct tggatcttcg gttcttggtt ctttgcattt
//
LOCUS       DAOPMW010000323         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27735_length_3894_cov_8.062256, whole genome shotgun sequence.
ACCESSION   DAOPMW010000323 DAOPMW010000000
VERSION     DAOPMW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27735_length_3894_cov_8.062256"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1577)
                     /locus_tag="PLU11_12785"
     CDS             complement(24..1577)
                     /locus_tag="PLU11_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPN60052.1"
                     /translation="MPIQHIFFVYLVSTLIVFLPSFGLAPLFVKAGVPAWKAYVPFYN
                     TWVMQGLCQRPKHWVFWQFIPVIGWFISPGIFIEFAKVFGQFSLRSHTAAALFAPFYF
                     PYLSMKVKPSYIGPEAVKNHKKADWREWVDAGIFAVVAATLIRTFIFEAYTIPSGSME
                     KSLLINDFLFVSKFSYGPRIPNTPLSVPFVHNYLPFGSSRKSYSEWVEIPYIRWFSTP
                     VKRGDAVVFNFPAGDTVVNRPGYQSFKEYYTLCRDLGDGDMAKGRAFILNSDTLPLAV
                     HPVDKTDNYIKRCIGVSGDLLEIRKGEVYINGQLQKMPDHSIMSYQVETNGQVLDPEI
                     LKSEYGVNYDEEVASVGMNVYYFRNLTAKALADMKAAGLVKRAEPELVPADKMDFSNF
                     RNQVYPYDTLHKWTIDDFGPIWIPKKGATLQLTPKDYSIYERAIRVYEKNEFYRKDNA
                     FFLNGKEVKSYTFKMDYYWMMGDNRHGSQDSRYWGFVPEDRVVGKAWLIWFSWEGGPR
                     WKRLFRTVK"
     gene            1702..2592
                     /locus_tag="PLU11_12790"
     CDS             1702..2592
                     /locus_tag="PLU11_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPN60053.1"
                     /translation="MQQFSIRDIEHLCGIKAHTLRAWEQRYQLGISDRLGGQHRVYSN
                     EDLKEWLRISYLYHNGCKISTIAAMSQTEREARLEKAYETDNPDEGFIHQLIEASLDF
                     NKEQFEKIIHCVVLRNGIEHCVQRIFYPYLHRIGLLWLTNHAIPAQEHFASHIIRKKM
                     LVATDGLEKPEREGPRILLFAPAGEYHEIPLLTAQYFFKKAGFKTVYFGTNVSAESLV
                     YYISNHPVDYLYTHVITFLQETSPEAYLLELKKHFKGKIIRSGPAFRLLPDDSTGEIV
                     LHSLEELILLTRNLTSQPTI"
     gene            complement(2585..3745)
                     /locus_tag="PLU11_12795"
     CDS             complement(2585..3745)
                     /locus_tag="PLU11_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN60054.1"
                     /translation="MADILIIDDERAIRKTLSEILSFEGYKVDEASDGEEGLKKFREK
                     TFDLVLCDIKMPKLDGIEFLQRAGEINPDIPIIMISGHGNIETAVEAVKKGAYDFISK
                     PPDLNRLLITIRNAMDKSTLVTETKVLKRKVSRVQEMIGESAGIRKIKDTIEKVAPTE
                     ARILITGDNGVGKELVARWIHEKSNRASGPIIEVNCAAIPTELIESELFGHEKGSFTS
                     AIKQRIGKFESANGGTLFLDEIGDMSANAQAKVLRALQEGKITRVGADKDINVDVRVI
                     AATNKDLLKEVEAKNFRLDLYHRLSVILIHVPSLNDRRDDIPLLVDQFLTDICADYGI
                     ARKHTEPEAISLLQEYNWTGNIRELRNVVERLVILSAKSITAEDVRSYVLPK"
BASE COUNT         1044 a          959 c          842 g         1049 t
ORIGIN      
        1 catcaagggc atttaaagtt agtctatttt acggttcgga acaatcgctt ccacctcggt
       61 cctccttccc agctaaacca gatcagccag gcttttccga ccacccggtc ttccggaaca
      121 aaaccccagt aacgggaatc ctgtgaccca tgtcggttgt ctcccatcat ccagtaataa
      181 tccattttaa atgtataaga tttcacttct tttccattca ggaaaaaggc attatccttc
      241 ctataaaatt cattcttttc atacacccgg atcgcccgtt cataaataga ataatctttg
      301 ggcgtcagtt gcagggtggc tcctttttta gggatccaga tcggaccaaa atcatcgatg
      361 gtccatttat gcaatgtatc gtaaggatat acctggttcc tgaaattact gaagtccatt
      421 ttatcagccg gcactaattc cggttcggct cttttcacca acccggctgc tttcatatcg
      481 gccagggctt tggctgtcag gtttctgaaa taatatacat tcatccccac actggccact
      541 tcctcatcgt aattcacgcc atattccgat ttcaggattt ccggatccag cacctgtccg
      601 tttgtttcca cctggtaact catgatggaa tgatcaggca ttttctgtaa ttgtccgttg
      661 atgtatactt cccctttccg gatttccagt aaatcaccgg aaacaccaat acaacgtttg
      721 atataattat ccgttttatc aaccggatgt acggctaaag gcaaggtatc tgaattcagg
      781 ataaaggccc ttcctttcgc catatcccca tcgcctaaat cacggcaaag ggtgtaatac
      841 tctttgaatg actggtaacc gggacgattg accacggtat cacctgccgg gaaattaaac
      901 accacggcat ctcccctttt aaccggggtg gaaaaccagc ggatgtaagg aatctctacc
      961 cattcagaat aggatttccg gctggagcca aagggcaggt aattatgtac aaatggaaca
     1021 gacaaggggg tattcggaat ccggggacca taactgaatt tgctcacgaa aagaaaatca
     1081 ttaataagca atgacttttc catggagccg gagggaatag tataggcttc aaaaatgaaa
     1141 gtacggatca gggtggcggc caccacggcg aaaataccgg catccaccca ttctctccag
     1201 tctgcttttt tatgattctt aactgcctca gggccgatat atgacggttt tactttcatg
     1261 gacaggtaag gaaagtaaaa aggggcaaac aacgcggccg ctgtatgtga gcgcaaagaa
     1321 aactggccaa acactttggc aaactcaata aatattcccg ggctgataaa ccatccgatc
     1381 accggtataa actgccagaa cacccaatgt ttgggccgtt ggcaaagtcc ctgcatcacc
     1441 caggtattat aaaatggcac ataggctttc caggcaggta ccccggcttt tacaaagagc
     1501 ggggccagcc cgaaggaagg caggaaaacg atcagggtgc tgactaaata gacaaaaaaa
     1561 atgtgttgga tgggcatgta cctgggttta tagctgacaa aaatatctta aatatttgcc
     1621 tttcagaaat atagattctt caaagttttg tgaattctga gctgttttca gtggaaataa
     1681 tgataattta ggctcgttca tatgcagcag ttttccatac gagacatcga acacctttgc
     1741 gggatcaagg cccataccct ccgggcctgg gagcaacgat accagttggg tatttctgac
     1801 cgcctgggtg gccagcaccg ggtctatagt aatgaagacc tgaaggaatg gctgcggatc
     1861 tcctatttat atcacaacgg ttgtaaaata tccaccattg cggccatgag tcagaccgag
     1921 cgggaagccc ggctggaaaa agcttatgaa acagataatc ccgacgaagg attcatccac
     1981 caactcattg aggccagtct tgacttcaat aaagagcaat tcgaaaaaat aattcactgt
     2041 gtggtattgc ggaatgggat tgagcattgt gtgcagcgaa tcttttaccc ttacctgcac
     2101 cggatcggct tactttggct gaccaaccat gcaattcccg cccaggaaca ttttgccagt
     2161 catatcatcc ggaaaaaaat gctggtcgcc accgatggac tcgagaagcc ggaacgggaa
     2221 ggtccccgta tccttttatt tgctccggct ggggagtacc atgaaatccc gttactgact
     2281 gcccagtact tttttaagaa agccggattt aagacggtgt attttggcac caatgtttcc
     2341 gcagaaagcc tcgtctatta tatatccaat cacccggtgg attatcttta tacgcatgtc
     2401 atcacctttc tacaggaaac cagtccggaa gcctatctgc ttgaattaaa aaagcatttt
     2461 aaaggaaaaa taatccggtc aggacctgct ttccggctat taccggatga ttcaaccggt
     2521 gaaatcgtac tgcattcact ggaagaatta atcctgctaa cgcgaaacct gacgagccaa
     2581 ccaactattt aggcaataca taactcctga catcttcggc ggttatggat ttggcggaca
     2641 gaattaccag gcgttccacc acgttccgca gttcccggat atttccggtc cagttgtact
     2701 cctgcaaaag actgatggct tctggttccg tatgtttcct ggcaatcccg taatctgcac
     2761 agatatccgt aagaaactga tccaccagta aggggatatc gtcgcgccgg tcattcaggg
     2821 agggtacatg tatgaggatt acactcagac ggtgatacag gtcaagacgg aaattctttg
     2881 cttccacttc cttcagcagg tctttattag tggccgcaat cacccgtaca tccacattaa
     2941 tatccttatc tgcacctacg cgggtgatct tcccttcctg cagggcccgg agcactttgg
     3001 cctgggcatt ggcgctcata tccccgattt catccaggaa taaagtgccc ccattggccg
     3061 attcaaactt cccgatccgt tgtttgattg cggaagtaaa cgaccctttt tcatgaccaa
     3121 acaattcact ctcgatcaac tctgtgggga tggccgcaca attcacctcg atgatcggtc
     3181 cggaagcccg gttacttttc tcgtgaatcc aacgggctac cagttccttt cccaccccat
     3241 tatcaccggt tatgagaata cgggcctcgg tcggggctac tttttcaatg gtatccttga
     3301 tttttctgat accggctgat tcaccgatca tttcctgcac ccggcttact tttcgcttca
     3361 gcaccttggt ttcggtcacc aatgtgcttt tatccatagc attccggatg gtaatcagga
     3421 ggcggttcag atccggaggc ttggagataa agtcataggc ccctttttta acggcttcca
     3481 cggccgtttc aatattgccg tgcccggaaa tcatgataat cggaatatcc gggttaatct
     3541 ctccggccct ttgcagaaat tctatcccgt ctagtttggg cattttaata tcacataaga
     3601 ccaggtcaaa ggtcttttcc ctgaactttt ttaatccttc ctcgccgtca gaagcctcgt
     3661 ccactttata accttcaaaa gacagtattt ctgagagggt tttccggatg gccctttcat
     3721 cgtcgataat caggatatca gccattatat tcgttttagg tataaaatta aatcatttgt
     3781 cccgaaggca gggtaattca gggattggaa ggggggtaaa aaaatagagg ccgggtagaa
     3841 acccagcctc gttttaaaat ccaatatcct atgaaaaacc gagacaaaga tgca
//
LOCUS       DAOPMW010000324         3886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27808_length_3886_cov_10.367267, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000324 DAOPMW010000000
VERSION     DAOPMW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27808_length_3886_cov_10.367267"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PLU11_12800"
     CDS             <1..1094
                     /locus_tag="PLU11_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN60055.1"
                     /translation="ALIKGAQLPFSKWLLGAMVAPTPVSALLHSSTMVKIAPFIILRL
                     SPALKDTPVAMVIIGLTGFVFIASAISALSQDNFKRILAHSTIALLALMIMMGAVGTP
                     VTVVASLVLVLFHGISKCMLFLNAGVLERVFHLKQSSDMDRLGESGPFTALVMTIGFM
                     SLLLPPFGAFIGKWLSIETLGALATDKRLLGALVIVAVAGGGAVLSLLYFKVMGLLIN
                     RSGEKDHIKFEKTGPYYAGTIYILLGLVLATMAGLPLLLTYYFAPVASATIGQPVPVL
                     TEGWNLFIGTMRIPLLPMLIAFFLLPVTVVVAMFIRFRQVDRAKEYMCGEKVNYAFSS
                     FYFSTDRAIPYFSAAGILFFIALIMVALI"
     gene            1105..1944
                     /locus_tag="PLU11_12805"
     CDS             1105..1944
                     /locus_tag="PLU11_12805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPN60056.1"
                     /translation="MNEYLIPVLLIVLAPVIGGLVYGFDRIVKARMQRRIGPPLLQPF
                     YDFLKLADKRQMMVHSTHAFLGIMHFVSLWFALAVLLLGGDLILVIFLHLLSTSLLIL
                     AGYSTRSVFSHLGANRMAISVLAYEPVLIIIAVSIFMINGSFDTSVVFASDQPLLYKM
                     PLAFIALLLILPIKLKKSPFDVAEAHQEITGGVEIEYSGLFFEAVYTAKWLDYIFCYT
                     LVFLFAGSNILLGIGLVVFTFFYLAALDNSTARVNYKQMLRFSLGVAFTLAFINLLIN
                     SLI"
     gene            1941..2465
                     /locus_tag="PLU11_12810"
     CDS             1941..2465
                     /locus_tag="PLU11_12810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPN60057.1"
                     /translation="MNLASYRKKSPWILHFNAGGCNGCDIEILACLGPKYDIERFGMI
                     NTGNPKQSDILLITGPVTKRTRERLVEIYAQMPEPKVVISCGACASTGGVFRGMYNCE
                     GGVDQFIPVDVYVCGCGTRPEQIIDGVVQALGILEKKTKALEESVKLKKEAWQEDGQL
                     NRRNIADEIKPLLA"
     gene            2462..2803
                     /locus_tag="PLU11_12815"
     CDS             2462..2803
                     /locus_tag="PLU11_12815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN60058.1"
                     /translation="MKKIETTLANLRKDIAAYYKPELHHFMVMNAVETGEQMEVQWFF
                     ADYAHPCATTCFYTMIGRDEEVPSIKDIVLSAWVAEAELVDLMNINVENTAKGFVLEP
                     DFESGPLLKKK"
     gene            2805..>3886
                     /locus_tag="PLU11_12820"
     CDS             2805..>3886
                     /locus_tag="PLU11_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPN60059.1"
                     /translation="MGRQYEIPVGAQHISLLEPLHFKFTTENEKIISTDANVYYVHRG
                     IEQACVSKFKFRQVSFVVGRVCGLCSISHTTAYSQVAEKLLKIEIPKRAKYLRMLVIE
                     LDRIHSHLLCLSHVAENSGFEALFMKTMQYREFSMELLEAVCGNRIQYDFSIVGGVAR
                     DLKPEIGQLILQRLKTFMPQLEELVNIYENNWTLSLKNKGVGVLTKQDAMLLNVVGPF
                     ARAAGLAVDVRDETEDLLPWKEVGFEMITDTAGDVHARNMIRLREIYVSIRIIQNIIN
                     GLPEGELVTETKAFPDGEATVRLEAPRGELFYYAKGNKTQVLERLKIKAPTFSNVAGM
                     VKAFEGNEYGSAPAIIASYDPCLSCTA"
BASE COUNT          938 a          861 c          994 g         1093 t
ORIGIN      
        1 ccgccttaat taaaggggcc cagctgccct tcagtaaatg gttattggga gccatggtgg
       61 cccccacacc ggtgagtgcc ttactgcact cctcaacgat ggtaaagatt gcaccattta
      121 ttatacttcg tttatccccg gccttgaaag atacacctgt ggccatggtc atcatcgggc
      181 tgaccggatt tgtatttata gcttcagcga tcagtgcttt gtcgcaggat aatttcaaac
      241 gtatcctcgc tcattccacc attgccttac tggccctgat gattatgatg ggagccgtgg
      301 gaacgcccgt aacagtagtg gcctcattag tgctggtact ctttcatggt atatccaagt
      361 gcatgctctt cctgaatgca ggtgtgctgg aacgggtttt ccatctcaaa caatcctccg
      421 atatggatcg cctgggtgaa agcggacctt ttaccgccct ggtgatgacg atcggtttta
      481 tgtccttatt actgccgccc tttggcgctt ttatcggcaa atggctgagt attgaaacat
      541 tgggagccct ggctaccgat aaacgattac tgggtgcttt ggtgattgtt gcggtggcag
      601 gtggtggcgc tgttttgtcc ctgctttatt ttaaggtaat gggattgctg atcaaccgca
      661 gcggcgaaaa ggatcatatt aagtttgaaa agaccggacc ttactatgcc ggcaccatct
      721 atatattact cggactggtc cttgccacta tggcgggcct gcctttatta ctcacttatt
      781 attttgcgcc ggtggcttcg gccaccatcg gacaaccggt gccggtgctg acggagggat
      841 ggaacctgtt tatcggtacg atgcgtatcc ccttattgcc aatgctgatc gctttctttt
      901 tattgcctgt taccgtggtg gtggccatgt ttatccggtt tcgccaggtg gacagggcta
      961 aggaatatat gtgcggggaa aaagtgaact atgctttttc ttctttctat ttttcaacag
     1021 acagggctat cccgtatttt tcagccgcgg gtatcctgtt ttttatagcg ctgatcatgg
     1081 tggcattgat ttaaaacgaa tgttatgaat gagtacctga tcccggtttt actgattgta
     1141 ctggccccgg ttattggcgg actggtttat ggctttgacc gtattgtgaa agcccgtatg
     1201 caacgcagaa tcggacctcc ccttttacag cctttttatg atttcctgaa actggccgac
     1261 aaacggcaga tgatggtgca ttccacccat gctttcctgg gcatcatgca ttttgtttcg
     1321 ctctggtttg ccctggcggt cttgttactt ggtggcgacc tgatcctcgt gatcttcctg
     1381 cacctcctgt caacttcttt attgatcctg gctggttaca gtacccgctc ggtcttcagt
     1441 catttggggg ccaaccggat ggccatcagt gtattggcat atgaacccgt gctgattatt
     1501 atcgccgtat ccatctttat gatcaatggc agttttgata catcggtcgt gtttgcttct
     1561 gatcagccct tgttatataa aatgccgctg gcttttattg ccctcttact gatcttaccc
     1621 attaaactga aaaagtcacc ctttgatgtg gccgaagccc accaggagat taccggcgga
     1681 gtggaaattg aatattccgg tttattcttt gaagcggtct ataccgccaa atggctggat
     1741 tatattttct gctataccct tgttttcctt tttgccggca gcaatatttt actgggtatt
     1801 ggcttagtcg tctttacctt cttttacctg gccgccctgg ataattccac ggcaagggtt
     1861 aattataaac aaatgctgcg tttttcgctg ggagtggcat tcactttagc ttttattaat
     1921 ctattaatca atagcctcat atgaacctgg cttcctacag aaaaaaatca ccctggatcc
     1981 tgcattttaa cgcgggagga tgcaatggct gtgatattga gatcctggcc tgcctgggtc
     2041 ccaagtatga tattgaacga ttcgggatga tcaatacggg taacccgaaa caatcggata
     2101 ttttattaat caccgggccg gtgaccaaac gtacccgcga acggctggtg gagatctatg
     2161 cacagatgcc cgagcccaaa gtggtgatca gttgcggtgc ctgtgccagt actggtgggg
     2221 tcttcagggg tatgtacaac tgcgagggtg gagtagacca gtttatcccg gtggatgtgt
     2281 atgtatgcgg ttgtggtacc cgtcccgaac agatcattga tggagtagta caggccctgg
     2341 gcatactgga aaagaaaaca aaagcactgg aagaatctgt aaaactgaaa aaagaagcct
     2401 ggcaggaaga tggccagctg aaccgaagga atatcgctga tgagataaaa ccattactgg
     2461 catgaaaaaa attgaaacca cattagccaa cctgcgcaag gatattgcgg cctattataa
     2521 accggagctc catcatttta tggtgatgaa tgcggtggaa accggcgaac aaatggaagt
     2581 gcaatggttc tttgcggatt atgcacatcc ttgcgccacc acttgtttct ataccatgat
     2641 cgggcgggac gaagaagtgc ccagtatcaa agacattgtt ttatctgcct gggtggcgga
     2701 ggccgaactg gtggacctga tgaatattaa tgttgaaaac acagcgaagg gatttgtcct
     2761 ggagcctgat tttgaaagcg gaccattatt aaagaaaaaa tgatatggga aggcaatatg
     2821 aaataccggt aggcgcccag catatatcac tgctggagcc cctgcatttt aagtttacga
     2881 cggaaaatga aaagatcatc agtacggatg cgaatgtata ctatgtacac cggggcatcg
     2941 aacaagcctg tgtgagcaag ttcaaattcc gccaggtgag ttttgtggtg ggacgtgttt
     3001 gcggattatg ctccatctcg catacgactg cctattctca ggtagccgaa aaattactga
     3061 agatcgagat tcctaaaaga gcgaagtacc tgcggatgct ggtcattgaa ctcgaccgga
     3121 tccactcaca cctgctttgt ctgagtcacg tagcggagaa ctccgggttt gaagccttat
     3181 tcatgaagac catgcagtac cgcgaatttt ctatggaact gcttgaagcc gtttgcggta
     3241 accggataca gtatgatttt tccattgtgg ggggtgtagc ccgtgacctg aaaccggaga
     3301 tcggtcagct gatcctgcaa cgactgaaaa catttatgcc ccagctggaa gagctggtga
     3361 atatatacga aaacaactgg acgctgtccc tgaagaacaa aggcgtgggg gtactgacga
     3421 aacaagatgc gatgttgttg aatgtggtgg gtccttttgc acgggcagct ggtttggcgg
     3481 tggatgtacg cgatgaaacc gaagacttat tgccctggaa agaagtgggc tttgaaatga
     3541 tcacagacac ggctggtgat gtgcatgccc ggaatatgat ccggctgcgc gagatctatg
     3601 tgtccatccg gatcattcag aatattatca atggtttacc ggaaggtgaa ctcgttaccg
     3661 aaacaaaagc ttttcctgat ggggaagcca cggtacgtct cgaagctccg cgtggtgaat
     3721 tattttatta cgcaaaggga aacaagacac aggtactgga acgcttaaaa ataaaagcac
     3781 cgactttttc caatgtggct ggtatggtaa aggcctttga aggaaatgaa tatgggtccg
     3841 ccccggcgat cattgcgtcg tatgatccgt gtttgtcgtg tacggc
//
LOCUS       DAOPMW010000325         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27860_length_3881_cov_4.967852, whole genome shotgun sequence.
ACCESSION   DAOPMW010000325 DAOPMW010000000
VERSION     DAOPMW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27860_length_3881_cov_4.967852"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PLU11_12825"
     CDS             complement(<1..1635)
                     /locus_tag="PLU11_12825"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPN60060.1"
                     /translation="MQLKGLVRFFTVLLIVYSLYQLSFTWFVKNHEKKMEAKAQEYVK
                     KNFPTAAVKFPGDKKAQEAYQEELDKIRTRYSDSLIKATENTTVTYGINGAMSYKKAK
                     EEELNLGLDLQGGMNVTLEVEMTGLLTSLSNGSTDPNFLTALKNADARKGNSDADFIT
                     LFIQEFRKLDANKPLASIFAGGSNNVIDVKSSDKKTEDYIRDQAKTAFNLTFDRLSTR
                     IDQFGVAQPSINPIPSRGLINVELPGIKDKERVRKLLQSTANLQFWEVYTLEDKNYET
                     GWQRTIDAYNAKYAGVKDTTAVKDSAAKSTDTAGTKIKDTTKTANINDMVDTGKKKTD
                     TSATAAADVNVINKFIQFDLPRQDEKGIMRFPAWIGYVMIRDTAAFRNYLTEFKSNFP
                     SDAVFAYGIPDKDKKGVAVEFVPLYALKTFNRTTAKLEGDAISDARQDFDQYGKVAVQ
                     MNMKPQGAAIWKKMTEDNVGKPLAIVLDNVVQSAPNVNNVIPSGESSITGSYTQQEAS
                     DLANILKIGKLPAPAKIVQEQQVGPTLGAKAIEGGSM"
     gene            2654..3844
                     /locus_tag="PLU11_12830"
     CDS             2654..3844
                     /locus_tag="PLU11_12830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPN60061.1"
                     /translation="MQLSSFLQRFNEPETLKMAKLGRELRAKGIDVIDLSLGEPDFDT
                     PEHIKQAAVKAIQDNWSHYTPVSGYPDLREAVCTKLKRDNNLDYKAENIVTSTGAKQS
                     LANAILALVDDNDEVIIPTPYWVTYSELVKIARGKVVEVKSSPATGFKINAAQLEAAI
                     TPNSKVFLFSSPCNPSGAVYSKEELEALAAVFLRHPQILIISDEIYEYINFVGKHESI
                     AQFADLKDRVIIVNGLSKGFAMTGWRLGYIAAHADIAKACEKIQGQFTSGTNSITQKA
                     AVVALTTDLRPTMEMVEEFTRRRSKVLEIVKDIPGVICPEPEGAFYIFPDVSYYYGKA
                     DGDNTISNAADLSMYLLNTAHVSSVMGDAFGEPKCVRFSFANSMEKIEEGWKRIKVAL
                     AKLK"
BASE COUNT         1006 a          880 c          955 g         1040 t
ORIGIN      
        1 catgctgcct ccttcgatgg ctttggctcc gagagtgggt cctacctgct gttcctgaac
       61 aatcttggca ggggcgggga gtttaccgat tttcaggata ttcgccagat cggacgcttc
      121 ctgctgggta taactaccgg tgatggatga ttcaccactg ggaattacat tattaacatt
      181 cggggcagac tgtaccacat tatccagcac aatggccagg ggtttgccca cattatcttc
      241 ggtcattttt ttccagatgg cagcaccctg gggcttcatg ttcatctgta cggctacttt
      301 accgtactgg tcaaagtcct ggcgggcatc agaaatggca tccccttcca gtttggccgt
      361 agtccggtta aaggttttaa gcgcatagag cggaacgaat tccacagcca cgcctttttt
      421 atctttatca ggaataccgt aagcaaaaac cgcatcactg gggaaattgc ttttaaattc
      481 agttaagtag ttgcggaaag ctgccgtgtc acggatcatt acataaccga tccaggcggg
      541 gaaacgcata atccctttct catcctggcg gggcaggtca aactggataa acttattgat
      601 tacattgaca tcggccgcag cggttgcgga ggtatctgtt tttttcttac cggtatccac
      661 catatcattg atattggcgg tcttggtggt atcttttatt ttagtgccag ccgtatcagt
      721 tgattttgca gcagagtctt ttaccgctgt agtatctttt acgccagcat atttagcgtt
      781 gtaagcatcg attgtgcgct gccacccggt ttcgtaattc ttatcttcca gggtatacac
      841 ttcccagaac tgcaggttgg ccgtactctg taacagctta cgtacccttt ctttgtcctt
      901 gataccgggc agttccacat tgatcagacc gcggctggga atcgggttga tggaaggctg
      961 ggccacacca aactggtcga tacgggtact taaacgatca aaggtcaggt tgaaagcggt
     1021 cttggcctgg tcgcggatat aatcttccgt ttttttatcg ctcgatttta catcgatcac
     1081 gttattactt cccccggcaa aaatggatgc caagggcttg ttcgcatcaa gcttgcggaa
     1141 ttcctggatg aacagggtga tgaaatctgc atcactgttc cctttccgtg catctgcatt
     1201 tttcaaagcg gtcaggaagt ttggatcagt ggagccattg gacaatgaag tcagcagtcc
     1261 ggtcatctcc acttcaaggg tcacgttcat tccaccctgg aggtcaagac cgagattcaa
     1321 ctcttcttct ttggctttct tgtagctcat agccccgttg ataccgtaag taacggtcgt
     1381 gttctccgtg gctttgatca ggctgtccga ataacgggtg cggatcttgt ccagttcttc
     1441 ctggtaagct tcctgcgctt tcttgtcgcc ggggaatttc actgccgcag tggggaaatt
     1501 cttctttaca tattcctgcg ctttggcttc cattttcttt tcatggtttt ttacaaacca
     1561 ggtaaaagag agctggtaca gcgagtacac aataagcagc acagtgaaaa agcgaaccaa
     1621 acctttcagt tgcatacgtt gatcgattta cattttatcc cgcacaatcg ttgatgcctt
     1681 tgggataaag ggtttataga aattttaaaa gcagtcccgt aacagaataa ggcccgttac
     1741 gggcgggcaa agatagtttt ttcggatcaa ttcccggcaa ggaaaaaagg gttcttgtag
     1801 actgattttt aaaaacttaa cggggggcaa ttcatgcctt taaccagcga tcttttccgc
     1861 tcaacggaga ctgcttttgg gccttttttg ggggctgccg gaatcttaac tttcaggcag
     1921 gatcagtcgg tttctggctg cttttaccat ccgggaaggg agaaaatgaa gtccaggcca
     1981 atgtctgatt cattcatata caatctttta cgaagcatcc cacccgggtt gagcccattt
     2041 tgaccaattt ggaacaattg gggaggcagg tcatttcctc ccgggtcgac cgggataaaa
     2101 agggccactc gtcttttttt aacaccggtt ccgggcgggg gctggcaggc gttttcatcc
     2161 ggccgctaaa gtaaaacaac cggttgccat cccggctttg cagcggctga acaagctgaa
     2221 aatacccgga ccggacaaaa atgccacccg gatcaagggt ccggaagtaa aaccgaagag
     2281 tataactttc tccggcgaaa taggtcaacg atgccgggag tatgatggct tcctgttcga
     2341 tgcagcgtat gcgaccgata cagtgaacca ggtggaatgt attttaagcg cagtgattta
     2401 ttgcagcaga gcagggatct tcaatgatga tcattttgag atgacagtac cggactgcca
     2461 ttcatgaaac aactggggca agtgataccg gtgtatgaac gaacaagagt taggaaatac
     2521 aaaccagatc tttttcaatt catatacaac tataaagatg gagttttatt aatcgttatc
     2581 tttgcaagcc gaatgtaagt acgaagtacg gagtacaaac actaaacttt caaccctcaa
     2641 cacgcaacac aaaatgcaat tatcttcttt cctgcaacgc ttcaatgaac cagagacttt
     2701 aaagatggcc aaactgggcc gtgaattaag agcaaaagga attgatgtta ttgacctgag
     2761 ccttggtgaa cccgattttg atacaccgga acatatcaaa caggccgccg taaaagcgat
     2821 ccaggacaac tggagtcatt atacaccggt atccggttat ccggatctgc gtgaagcggt
     2881 ttgtacgaaa ctgaaacggg ataataacct ggactataaa gcagagaata tcgttacttc
     2941 cacgggtgcc aagcagagcc tggccaatgc tatcctcgcc ctggtggatg ataatgatga
     3001 agtgatcatt ccaaccccgt attgggtaac ctacagtgaa ttggtaaaga ttgcccgggg
     3061 taaagtggtt gaagtgaaga gcagcccggc aacaggattt aagatcaatg cggcgcaact
     3121 ggaagcggca attaccccta attcaaaagt gttccttttc tcttctccct gtaatccgag
     3181 cggtgcggta tatagcaaag aagaactgga agcgctggca gctgttttcc tcaggcatcc
     3241 gcagattctc atcatctccg atgagatcta tgagtatatc aatttcgttg gcaaacacga
     3301 gagcattgca cagttcgctg atctgaaaga ccgggtgatc attgtcaatg gacttagtaa
     3361 aggatttgcc atgaccggct ggaggcttgg ttatattgcc gctcatgcgg atattgccaa
     3421 agcctgcgaa aaaatacagg gccagtttac cagtggcacc aactccatta cccagaaagc
     3481 agccgtggtg gcgctgacca cagatcttcg tccgacgatg gaaatggtgg aagagttcac
     3541 ccgccgccgc tctaaagtac tggagattgt gaaagatatt ccgggagtga tttgtccgga
     3601 gcctgaaggg gctttttata tcttcccgga tgtgagttat tactatggta aggctgatgg
     3661 tgataacacc atcagtaatg cagcagatct ctcgatgtac ctgctcaaca cagcacacgt
     3721 atcttctgta atgggggatg ccttcgggga accgaaatgt gtgcgttttt cctttgccaa
     3781 cagcatggag aaaattgagg aaggctggaa gcggatcaaa gtggcactgg caaaactgaa
     3841 ataagaaggt aaaagaagaa taaagaagag ccctgcagtg a
//
LOCUS       DAOPMW010000326         3878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27889_length_3878_cov_5.891708, whole genome shotgun sequence.
ACCESSION   DAOPMW010000326 DAOPMW010000000
VERSION     DAOPMW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27889_length_3878_cov_5.891708"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLU11_12835"
     CDS             <1..394
                     /locus_tag="PLU11_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60062.1"
                     /translation="IKDKETGRRSVILPDGAKLTIKGGSDYLGLIRSVSIIDGSTSQL
                     LSFSCNQVVLEHSSSSPTLASALDQLEADGETSAVEFTTDGLLWVNIYSENVQGTKVY
                     SRVKLGELKWAIPSNVTDHYDDPRIGHT"
     gene            773..2992
                     /gene="katG"
                     /locus_tag="PLU11_12840"
     CDS             773..2992
                     /gene="katG"
                     /locus_tag="PLU11_12840"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPN60063.1"
                     /translation="MENNTTQSTGGKCPFTGHTGKQTAGSGLGNREWWPNQLKLNILR
                     QRSSLTNPMEASFNYASEFKSLDLDAVKEDIVNLMTSSQDWWPADYGHYGPFFIRMAW
                     HSAGTYRIQDGRGGGGNGSQRFAPLNSWPDNANLDKARLLLWPVKQKYGRKLSWADLM
                     ILAGNCALESMGFKTFGFAGGREDIWEPEQDIYWGSETEWLGDKRYTGDRQLENPLGA
                     VQMGLIYVNPEGPNGNPDPLAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAAD
                     PSKYVGREPAAAGIEEQGLGWKNTYGTGHGVHTITSGLEGAWTTTPTKWSNNFFWNLF
                     GYEWELTKSPAGAQQWIPKHGMGKDTVPDAHDATKRHTPVMLTTDLALRVDPAYEKIS
                     KRFLDNPDQFADAFARAWFKLTHRDMGPRSRYLGKEVPAEVLIWQDPVPELTHELINE
                     SDIKTLKEKILAAGISVASLISTAWASASTFRGSDKRGGANGARIRLAPQKYWEVNNP
                     TQLSKVLDALEAIQKDFNSTAGNKKVSIADLIVLGGAAAIEQAAKSAGYTVSVPFTPG
                     RTDATQEQTDVESFAPLEPIADGFRNYVKKAHSAAAEDWLIDKAQLLTLTAPEMTVLV
                     GGLRVLNSNYDQSAHGVFTRNAGVLSNDFFVNLIDFGTTWKATSPANDLFEGLDRKTG
                     EVKWTGTRVDLIFGSNSELRALAEVYASADAKEKFVKDFIAAWAKVMNLDRFELAKG"
     gene            3351..>3878
                     /locus_tag="PLU11_12845"
     CDS             3351..>3878
                     /locus_tag="PLU11_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN60064.1"
                     /translation="MRKLLMLTTVLMYSICIMAQEKTVTGKVTDEKDNTPLSGVSVSV
                     KGTTIGTTTGTDGTFSLTVPETAKALHFTLVDYTEVEVLLAGKTNFTLTMASASKALS
                     EVVVVAYGNQKKESITGSVAVIGSEQLQTRLATNISQALAGAAPGISATSGNGQPGSS
                     AAIRIRGFGSVNASNS"
BASE COUNT         1050 a          807 c          957 g         1064 t
ORIGIN      
        1 cattaaggat aaagagaccg gccgccgctc agttatttta cccgatggcg ccaaactgac
       61 gattaagggt gggagcgatt acctgggtct catccgttcg gtcagcatca ttgacgggag
      121 taccagtcaa ttgctcagtt tttcatgcaa tcaggtggta ctcgaacata gctcctcttc
      181 acctacactg gcctccgcac tggatcagct ggaagccgac ggagaaacat cagctgtgga
      241 gttcactacc gacggattac tctgggtgaa tatatattcg gaaaatgttc agggaacaaa
      301 agtatacagc cgggtgaagt tgggtgaact aaagtgggcc ataccaagca atgtaaccga
      361 tcattatgat gatccccgca ttgggcatac ataactaact caaactttac tgattctttt
      421 tacaaccacc ggctttcaat tacagaaggc cggtggtttt ttattgccta caggtgaagt
      481 aatgaccggt ccgccagttt tctttcatca ataatttcta tcgggcgatt tgttttatct
      541 atcagccatt aaaaacgaaa tgaatatttt gtatataatc aattgataat aaattaattg
      601 tcaaagatgt ttgaagtatt gatttactct gtgcatgggt ggggtctgtc agcatttgtt
      661 gaaccgggta taactccttt tctggtattt tgcaatgttg tatcattgtc tgcttatcaa
      721 cattatctat ctgatttttt ttattattac taacatctat aattcttaaa caatggaaaa
      781 caatactaca caaagtacgg gaggtaagtg tccctttacc gggcataccg gcaagcaaac
      841 ggcaggcagt ggtcttggta accgtgaatg gtggcctaat cagttaaaat taaacatcct
      901 tcgtcagcgt tcttcgttga caaatccgat ggaagcttct tttaattatg catcggaatt
      961 caaatcactg gacctggatg ccgtgaaaga ggatatcgtg aatctgatga ccagttccca
     1021 ggactggtgg cccgctgact atggacatta tggtccattc tttatccgta tggcctggca
     1081 cagtgccggt acttaccgta ttcaggatgg tcgtggtggc ggcggcaacg gatcacagcg
     1141 gtttgcgcca ttgaacagct ggcccgataa cgcaaatctt gataaagccc ggttattgct
     1201 ctggcctgtt aaacaaaaat atggccgcaa gctttcctgg gctgacctga tgatcctggc
     1261 cggtaactgt gcgctggaat ccatgggctt taaaaccttt ggctttgctg gtggccgtga
     1321 agatatctgg gaacctgagc aggatattta ctggggttcg gaaacagagt ggctgggtga
     1381 caaacgttat accggtgatc gtcagttgga aaatccgctc ggtgcggtac aaatgggact
     1441 gatctatgta aatcctgaag gaccgaatgg caatcctgat cctttggcgg ctgcccgtga
     1501 tatccgcgaa acatttgccc gcatggctat gaatgatgag gaaacggtgg cattgattgc
     1561 cggtggtcat acattcggta aaactcacgg ggctgcggat ccttccaaat atgtaggacg
     1621 tgaaccggcg gctgctggaa ttgaagaaca gggtctgggt tggaaaaata cttatggcac
     1681 cggacatggc gtacatacga ttaccagtgg tcttgaaggc gcctggacta ccacacctac
     1741 aaaatggagt aataattttt tctggaacct tttcggttat gaatgggaac tgacgaagag
     1801 tcctgcaggt gcacaacaat ggatccccaa acacggaatg ggtaaggata ccgtgccgga
     1861 tgcacatgat gctacgaaac ggcatacccc ggtaatgctg acaacggatc tggctttacg
     1921 ggttgatccc gcttatgaaa agatttcaaa acgtttcctc gataatccgg atcagtttgc
     1981 tgatgctttt gcccgtgcct ggttcaaact gacacaccgc gatatgggac cccgttcccg
     2041 ttacctgggt aaagaagtgc cggctgaagt cctgatctgg caggacccgg taccggaact
     2101 gacgcatgaa ctgattaatg agtctgatat caaaacatta aaagaaaaaa ttcttgcggc
     2161 tggtatctct gtcgcttcat taatttccac tgcctgggct tctgcttcca ctttccgtgg
     2221 ttcagataag cggggcgggg ctaatggggc ccgtatccgc ctggcaccgc agaaatattg
     2281 ggaagtgaat aacccgaccc agttgtccaa agtactggat gcattggaag cgattcaaaa
     2341 agactttaac agtaccgctg gcaataagaa agtatcgatt gctgacttaa tcgtactcgg
     2401 tggcgcggct gctattgaac aggccgctaa aagtgctggt tatacagttt cggttccttt
     2461 cactcccggg cgtacggatg ctacgcagga gcagacagat gtggaatcat ttgccccgct
     2521 ggaacccatt gccgatggat tccgtaatta tgtgaaaaaa gcgcatagtg ctgccgcaga
     2581 ggactggctg attgataaag cccaattact gaccctgact gcccctgaaa tgaccgtact
     2641 ggtaggagga ttgcgggtgt tgaattccaa ctatgatcag tctgcacatg gtgtatttac
     2701 ccggaacgcc ggcgttctca gcaatgattt ctttgtaaat ctgatcgatt ttggtactac
     2761 ctggaaagcc acttcaccag ccaatgattt atttgaaggc cttgaccgca agaccggtga
     2821 agtaaaatgg acaggtaccc gggttgatct catctttggt tccaactctg agttgagagc
     2881 gcttgctgaa gtatatgcct ctgctgatgc taaggagaaa tttgtaaaag actttatagc
     2941 cgcctgggcc aaagtgatga atctggatcg gtttgaattg gcgaaggggt gagtggtgag
     3001 tggtgagtgg tgagtagtga gtagtgagta gtgagtagtg agtaacggtg ttataagaat
     3061 gattgtcatc ccaaatcgaa acgagggagc agcataggaa atggtggtct cagggccacc
     3121 atttttttta cattctttta gcgaaagcac ttgatttaaa ttcccaaatg catataataa
     3181 taaacacatg taaataaata taaatatata attatgagta aagcaaagcg aatatttatt
     3241 tttttgatga atggagcagc atttatatca taatagtgtt caatgtattg taattgtttt
     3301 tcaatgtttc tttatatttg aatcttaatc ttacattaaa aaaaatgcta atgagaaaac
     3361 tgctaatgct aacaactgtt ctgatgtatt ccatatgcat catggcacag gaaaaaacag
     3421 ttaccggaaa ggtaacggac gagaaagaca acacgccgtt gtctggtgtt tcggtctcgg
     3481 ttaaagggac cacgattggt actacaacag gcacagatgg aacgtttagt ttgactgttc
     3541 ctgaaacagc taaagccctt cattttacat tggtcgacta taccgaagta gaagtgttac
     3601 tggccggtaa gaccaacttt acattgacca tggcatctgc ttctaaagct ttatctgaag
     3661 tagtggtagt cgcttatggt aatcagaaaa aagaatcgat taccggttcc gtggcggtga
     3721 tcggatctga acaattacag acccgtcttg ccactaatat ttcacaggcg ctggcgggag
     3781 ccgctcctgg tatctctgcc acatcgggca atggacaacc gggtagcagt gcggctatcc
     3841 gtatccgtgg ttttggatcg gtgaatgcca gtaactct
//
LOCUS       DAOPMW010000327         3875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27915_length_3875_cov_12.390052, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000327 DAOPMW010000000
VERSION     DAOPMW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27915_length_3875_cov_12.390052"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLU11_12850"
     CDS             <1..657
                     /locus_tag="PLU11_12850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN60065.1"
                     /translation="ISIISHDIVTPLKFLNVAGKNLIEKRKLMSEDLQVETLKEITNT
                     SQELQLLSTNILNWIKYQNENRRLVKENFNLQEMVTQLLGLLQSLARQKKLIILNDVD
                     PALEIHQYYEPLKILIYNLLTNAIHFTEKGAIVVSAIKENGHITVSVKDEGIGMSPEQ
                     IQRLMAEEVVITSANVDNKKGHGLGYLIIKDLLKTMDASLDIQSKKGQGATVSIQMKN
                     "
     gene            701..1249
                     /locus_tag="PLU11_12855"
     CDS             701..1249
                     /locus_tag="PLU11_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60066.1"
                     /translation="MASTAQDTLPKLILVNQHRPSKKITLKNDWDTYFFTATGKYHGE
                     VIALTDSSLQLMRYYKNGKDSSYTKKVRVSRRHPEGLKIKHLSLYTRDTIQLLLKDIL
                     IVRKPWINKRGWMLIPAYLLGGAILAVPLLPVAAITKGSAGVRNWLQFEALLLGISGP
                     SLFIGTRHKKYVIGEKWKLVLE"
     gene            complement(1250..1717)
                     /locus_tag="PLU11_12860"
     CDS             complement(1250..1717)
                     /locus_tag="PLU11_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPN60067.1"
                     /translation="MYKKSLTKEQALQKLKHYCAYQERAHSEVKEKLYDLGVWKKDHD
                     EIISTLIEENYLNEERFAIAFAGGKWRVKHWGRVKIKYELKQKQVSEYCIKKAMKQID
                     EDEYMTVLKKMADERYASLKSDQYMVRKKKTMDFLMARGFEMDLIRKAVEDKA"
     gene            complement(2358..>3875)
                     /locus_tag="PLU11_12865"
     CDS             complement(2358..>3875)
                     /locus_tag="PLU11_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN60068.1"
                     /translation="KAGISTSRSNIRNKTLTIIEADTDGDGEYEALRANATFSDGSSA
                     DISSSIKKSPASGGSPSLTIDEAGTQRRRVEVLKSNKTGNPNARRLTGISVAGTGITL
                     RATATFSDGSSSDITESLEVNNAHNGVRQYSVMVSDVDGDGHADAVLKTRTKSNQTNE
                     RMAANAEEEIWSPRSNIKTIPVTTGDLDGDGAADMIAGNNGYDQTHRLTRGTKSGGAM
                     SRQAGGPIGGIIVKGGKNPGGQMRTMTTDENGMFEFTGLEAGSYTFTVEQKLIINDET
                     FVSVGDGSSSRAQDHNSSRSNKSGIVAPDGGGGSGGSGGTKAQDHNSTRSNKTASIAA
                     PDPGSGGGGGGGSKAQDHNSTRSNKTASVIDNDLDNGDGSFRKGDVKVTASQNTQSLR
                     TISVQADLDGDGVYETDVTSKISDELILDKEGNLTEPQQKAGISTSRSNIRNRSSLQP
                     VTQTVFTGYGTTTINGKEVEVKTVYKVVEKATSGLKDTLKTQVSIHPAKKYSPTV"
BASE COUNT          966 a          998 c          841 g         1070 t
ORIGIN      
        1 atctccatta tcagtcatga tatcgtgacc ccgttgaaat tcctcaacgt ggccggtaag
       61 aacctgatcg agaaaaggaa gctgatgagt gaggacctgc aggtggaaac cttaaaagaa
      121 atcacgaata cttcacagga actgcagcta ctctccacca atatcctcaa ctggatcaag
      181 tatcaaaatg agaaccgtcg tctggtaaaa gaaaatttca acctgcagga gatggtaacg
      241 cagttgctgg gtttattaca atccctcgcc cggcagaaaa aactcatcat tctcaacgat
      301 gtcgatccgg ccctggagat ccaccagtac tacgaacccc tgaagatcct gatctataac
      361 ctgctcacca atgcgattca ttttacggag aaaggcgcca ttgtggtatc cgccattaaa
      421 gaaaatggcc atatcactgt ctcggtcaaa gacgaaggta tcggcatgag tcccgaacag
      481 atccagcgcc tgatggccga ggaagtggtg atcacttccg ccaatgtcga taataagaaa
      541 ggccacgggc tgggttacct gattattaaa gatttgttga aaacaatgga tgcctcgctg
      601 gatatacaga gtaagaaggg gcaaggagct acggtgtcta ttcaaatgaa aaactaatat
      661 gcgtcgccat ctgtttatat ttctaagttt actgatatct gtggcttcaa ctgctcagga
      721 cacactgcca aaactgatct tagtgaatca gcaccgtcct tcgaaaaaga tcaccctgaa
      781 aaatgactgg gatacttatt ttttcaccgc cactggcaaa taccacggtg aagtcatagc
      841 actcaccgat tcctccttac aactaatgcg gtattataaa aacgggaaag acagcagcta
      901 caccaaaaaa gtgcgggtca gccgccgtca ccccgaagga ctgaagatta aacacctatc
      961 gctttatacc cgggatacca tacaattgtt gctgaaagat atcctgattg tacgcaaacc
     1021 ctggatcaat aagcggggct ggatgcttat tcctgcttac ctgttggggg gtgcgatact
     1081 ggccgtaccc ttactgccgg tggcagccat cacgaaaggc agtgccggtg tgcgaaactg
     1141 gctgcagttt gaagccctgc tgctgggcat ctccggacct tcccttttta tcggtacccg
     1201 gcataaaaaa tatgtgatag gagaaaagtg gaaactggta ttggaataat caggctttgt
     1261 cttccaccgc cttccgtata aggtccatct caaaacctct cgccatcagg aaatccatcg
     1321 tcttcttctt ccgaaccata tactgatccg attttaaaga ggcataccgt tcatcggcca
     1381 tcttctttaa cacggtcata tattcatcct catcaatctg cttcatggct ttcttaatac
     1441 aatactcact cacctgcttc tgcttcagtt catatttgat cttcacccga ccccagtgct
     1501 tcacccgcca cttgcccccg gcaaaagcaa ttgcaaaccg ttcttcattc aggtaattct
     1561 cttcaatcag tgtggagata atctcatcat gatccttctt ccacactcca aggtcataca
     1621 acttctcctt cacctcacta tgcgcccgct cctggtaagc acaatagtgc ttcagctttt
     1681 gcagggcctg ttctttggtg agggattttt tgtacatact gcgaagataa tacttcggca
     1741 agctcagtat gacaacgact gtcaccctga gcttgtcgaa gggcaccctt cgatacgacg
     1801 catgcttctg caagcctgcc tgtccggtag gcaggctcag catgacaagg actgttgtca
     1861 ccctgagttt atcgaagggc aggatgacac gatcatttcg ctccgaatgt tgaaaagcag
     1921 gaagtgtctg tatttcacta ccaaccccga agggggttga acgattaata gccccgggtg
     1981 tcaacccggg gtccgatgtt tacgatatct cctggaaccc tgaaggggtt caactaaatc
     2041 ttcgtatgta attattttgg cttgttatct gtaggacatt agatttaaaa aaactttaaa
     2101 agtttaatga attccgcctg cctatccggt agacaggttt atcgatggga tgggtgacat
     2161 agaggaaaca taataagttg aacccctccg gggttcgagg agatcgcggg aatatattaa
     2221 ccccgggttg acacccgggg ctattaatag ttcaaccctt tcagggttat gagagaatgt
     2281 ttattgaaaa tccttcccgc ttttcctctt acgaagcgga atgttgttgt caggttgagt
     2341 agtcccgcca aaaaaattta cacggttgga gaatactttt ttgcgggatg tatcgaaacc
     2401 tgcgtcttca acgtatcctt caacccgctc gtcgctttct ccactacttt atacaccgtt
     2461 tttacttcca cctctttccc gttgatagtg gttgtaccat aaccggtgaa aacggtctgg
     2521 gtgacgggtt gtaatgaact acggttgcgg atattgctgc gggacgtact gataccggct
     2581 ttctgttgcg gttcagtcag gttaccttcc ttatccagta ttaattcatc cgaaattttg
     2641 gaggtcacat ctgtttcata aacaccatca ccgtcgaggt cggcctgtac agagatggta
     2701 cgtaatgatt gtgtattctg ggaagcagtg actttcacat ctcctttacg gaagctgcca
     2761 tcgccgttat ccaaatcatt atcaatcaca ctggccgttt tattcgaacg cgttgaatta
     2821 tgatcctgtg ctttgctgcc gccgccgcca cctccaccac ttccgggatc aggtgctgca
     2881 atactggcgg ttttattgga gcgggttgaa ttatgatcct gtgctttagt tccgccgctg
     2941 ccaccactgc cgccgccacc atccggagca actataccgg atttattaga tcttgatgaa
     3001 ttgtgatcct gtgcacggct gctactgcca tcacctacag aaacaaatgt ctcgtcgttg
     3061 atgatcagtt tttgttctac cgtaaaggta taactgcctg cctcaaggcc ggtaaactca
     3121 aacatcccgt tttcatcggt ggtcatggtc ctcatctgac cacccgggtt cttgcctcct
     3181 ttaacaatga tccctccgat cggaccaccc gcctgccgcg acatagcacc accggattta
     3241 gtacccctgg tcaaccggtg ggtctgatca tatccattgt tacccgcgat catatccgct
     3301 gcaccatctc catccaggtc accggttgtt actggtattg ttttaatatt cgaccgggga
     3361 ctccagatct cttcctctgc attggcggcc atccgttcat tggtctgatt agatttcgtt
     3421 ctggttttca gcacggcatc tgcgtgtcca tccccatcca catcgctcac cataacacta
     3481 tactgacgaa ccccgttatg cgcgttgttt acttccagac tctccgtaat atctgatgag
     3541 ctaccatcag aaaaagtggc ggtagctctt aaagtgattc ctgtgccggc gacactgata
     3601 ccggtcagtc ttctggcatt cggatttccc gtcttatttg attttaatac ttccaccctg
     3661 cgccgttggg tacccgcttc atcaatcgtc agggaaggtg aaccacctga ggcaggagat
     3721 tttttaatgg atgaactgat atcggctgaa ctgccatcac tgaatgtcgc attcgccctt
     3781 aaggcttcat actccccatc accatctgta tcggcttcaa taatcgtcag cgttttattc
     3841 cggatattcg accgggaggt actgataccg gcttt
//
LOCUS       DAOPMW010000328         3868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_27976_length_3868_cov_4.077367, whole genome shotgun sequence.
ACCESSION   DAOPMW010000328 DAOPMW010000000
VERSION     DAOPMW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27976_length_3868_cov_4.077367"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLU11_12870"
     CDS             complement(<1..611)
                     /locus_tag="PLU11_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60069.1"
                     /translation="MNRNNLHLSLHYRVAWKRLSGFLSICLLMSCSLLAQTEDPPPIV
                     EEDTTETRTTIEVAPVETVEEEATYNQFLPLSETDSIRLQLRKVPDSVVTGLQEEDEF
                     WYANNRIQKKKPETNKPQRNYNPYSGSSGFTTLLWVLIVGLFLGVVIYYMAESGLFRR
                     RIKIKADQGQDLEEMPEDIFAINYQKEIDKAVANGDYRLAVRL"
     gene            complement(611..1579)
                     /locus_tag="PLU11_12875"
     CDS             complement(611..1579)
                     /locus_tag="PLU11_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPN60070.1"
                     /translation="MREALFIKKNKDRWLKNQHLPPDDPDEMAASFTQLVDDLAYAKT
                     FYPSGKVTHYINSQASRIYLDIYKNRKDDSNRIWKFWRYDLPLTIRKHHGIMLFATLF
                     FILFFVIGFFTASADETVAAEVLGPGYIEETHKNITKGNPFGIYEGGNSILTWLGIMV
                     NNLLVALRMFASGLAMGIPTLYSLAETGVMVGVFDQIFAAKGLGADFWLVVFVHGTLE
                     ITAIILTGGAGLVLGKGFLFPGTIRRIDAFKQSAKDGTKIMIGLLPVFAVAAFFEGFI
                     TRLYNDLSWLTTAVFSVSVIFVIWYFIIYPIRLHKRLSVQLNEEEA"
     gene            1675..2490
                     /locus_tag="PLU11_12880"
     CDS             1675..2490
                     /locus_tag="PLU11_12880"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPN60071.1"
                     /translation="MAVIKIPTNFNIDLEFEVPEFFRRILALVIDTILLIFYFKISFA
                     VLISMARNMNEGIEDNLQNLHWTYIFMVMGPIFFYHIIMEILLNGQSVGKKIMQLRVV
                     NENGGRASISQLFIRWMLRDVWFVMILLMGLNKDYMGEKTEAVLLILAVVGFFIADII
                     LVVSSKKGQRIGDLLAKTIIIRTQTKGDIEDTVFIEVADSYVPSYPQIMQLSDRDINS
                     IKSILETARKRGDYAMAESAAAKIKAHLNIETNMAAFDFLDVLLKDYNYLSTN"
     gene            complement(2578..3018)
                     /locus_tag="PLU11_12885"
     CDS             complement(2578..3018)
                     /locus_tag="PLU11_12885"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPN60072.1"
                     /translation="MENSSSNNSLFDLSFDNNVKSGLRDMAVWAGLAAIVSLINSIMG
                     LISYFIQVNKINKMVGDYPGTQRAMAGGLIWPLISLGIGVALFVFLNKFSKMAKSGVD
                     TNDSYLINEALGNLSTYFKFVGILIIIVLVIFLLVFMFGLAMGA"
     gene            complement(3034..3456)
                     /locus_tag="PLU11_12890"
     CDS             complement(3034..3456)
                     /locus_tag="PLU11_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60073.1"
                     /translation="MEPNQEQSLFDTSMDHVTQEHLQSVAKWTRFISITGFVSLGLVL
                     ILLLVAGSQLAGQLSTFSGFGGLDMAGAMIAIVIVALLIMGLWIYFLFKGSNLLSSGL
                     QNRDSAMLADGFRSMKTYFIISFVLSILSLLSALTTSF"
     gene            complement(3553..>3868)
                     /locus_tag="PLU11_12895"
     CDS             complement(3553..>3868)
                     /locus_tag="PLU11_12895"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPN60074.1"
                     /translation="LDGIAGYIDNLLLEIQQFMFDKAKAYRDANITPANSWEEFEKLL
                     DEKAGFISAHWDGTPETEEAIKEKTKATIRCIPLDNPKEEGTCIFSGKPSAERVLFAR
                     AY"
BASE COUNT         1073 a          908 c          852 g         1035 t
ORIGIN      
        1 tgcagccgca cggccagccg gtaatcccca ttggctacgg ctttatctat ctccttctgg
       61 taattgatag cgaaaatatc ctccggcatt tcttccaggt cctgaccctg atcggctttg
      121 atcttaatac ggcgccggaa taaaccactt tctgccatat agtagatgac caccccaagg
      181 aaaagcccga cgatcaatac ccaaagcagg gttgtaaaac cggaactccc gctatagggg
      241 ttgtaattcc gttggggttt atttgtttcc ggcttctttt tttgaatacg gttattcgca
      301 taccagaatt catcctcctc ctgcaatccg gtaaccacac tgtccggcac tttccgcagt
      361 tgcaggcgga tggaatctgt ctcgctgagg ggcaaaaact gattgtaggt cgcttcttcc
      421 tctaccgttt ccaccggtgc cacttcaata gtggttctcg tttctgtggt atcttcttcc
      481 actatcggtg gaggatcctc agtttgtgcc agtaatgagc aactcatcag caggcagata
      541 cttaaaaagc ccgacaggcg tttccaggca actctataat gcagtgaaag atgcaggtta
      601 ttccggttca ctaggcttcc tcctcgttta actgtaccga caaacgctta tgcaggcgga
      661 tgggataaat gataaaatac cagatcacaa aaatgacaga caccgaaaaa acagcggtcg
      721 tcagccagga taaatcatta tacagacgcg tgatgaatcc ttcaaaaaat gcagccaccg
      781 caaaaacagg cagaagaccg atcatgattt ttgtcccatc tttagcgctt tgtttgaaag
      841 catcaatccg cctgatcgtt ccggggaaaa gaaatccttt cccgagaacc agtccggcac
      901 cgccggttaa tatgatagca gtgatttcca gtgtgccatg tacaaaaacc accagccaga
      961 aatcagcacc tagtcctttg gctgcaaata tctggtcaaa cacgcccacc atcacacccg
     1021 tctctgccag gctgtacaaa gtggggatac ccattgccag tccggaagca aacatgcgca
     1081 acgccactaa caggttattg accataattc ccagccaagt gaggatactg ttcccccctt
     1141 catagatccc aaacggattt cctttagtta tgtttttatg ggtttcttca atataacccg
     1201 gtcccagcac ttcagccgct actgtttcat ccgcggatgc ggtaaaaaag ccgatcacaa
     1261 aaaacaggat aaagaaaagg gtggcaaaca gcatgatccc atggtgtttc cggatggtca
     1321 gcgggagatc atagcgccag aatttccaga tccggtttga atcatctttc cggttcttat
     1381 aaatatccag gtaaatgcgg gacgcctgtg aattaatata atgggtgact tttccggaag
     1441 gatagaaggt tttggcatag gccaggtcat ctactaattg ggtaaaggaa gccgccattt
     1501 catccggatc atccgggggg agatgctggt ttttcaacca gcggtcttta ttcttcttta
     1561 taaacagggc ttccctcaat ccgtgtattt tgctcgtaaa atactatttt taaaataaga
     1621 aaagcaggac ctgccgaata ttatttctcc cattcagtac ttacctttac cctgatggct
     1681 gttatcaaga taccgaccaa ttttaatatt gacctggaat tcgaagtacc cgaattcttc
     1741 cgccgtatcc tggccctggt aattgatacc attttactca tcttttattt taagatttca
     1801 tttgcggttc tgatttcgat ggcccgcaac atgaatgaag ggatagaaga caacctgcaa
     1861 aacctgcact ggacttatat ctttatggtg atggggccca tcttttttta ccatattatc
     1921 atggaaatcc tgctcaacgg ccagagtgtg ggaaaaaaaa tcatgcaact gagggtcgtg
     1981 aatgaaaatg ggggccgggc cagcatcagc cagctgttta tccggtggat gctgcgtgat
     2041 gtctggtttg tgatgatcct gctgatggga ctcaataaag attatatggg ggaaaagaca
     2101 gaagccgtgt tactgatcct ggccgtggtg ggttttttta ttgccgacat catcctggtg
     2161 gtttccagta aaaaagggca acgcatcggc gatttgctgg ccaagacaat cattatccgc
     2221 acccagacaa aaggggatat tgaagatacc gtctttattg aagtggccga tagttacgta
     2281 ccctcctatc cccagatcat gcagttgagc gaccgggata tcaattcgat taaaagcata
     2341 ttggaaacgg cccgtaaaag aggggattat gcgatggcgg aatcagccgc tgcaaaaatc
     2401 aaagcccacc tgaatatcga aaccaatatg gcggcttttg atttcctcga tgtactgtta
     2461 aaggattaca attatctctc caccaactaa cccattcgtt ttttaaataa aaaccgggaa
     2521 aacagcatcg ggtgatgccg ctttaccggt tgatatttta ttcgttaaat acgctactta
     2581 agcccccatt gccagtccga acatgaagac cagtaagaat atcacaagaa cgataataat
     2641 cagaatcccg acaaacttga agtaagtaga caggtttccc agtgcttcat tgatcaggta
     2701 gctgtcattg gtatccacac cggatttcgc catctttgag aatttgttca agaagacaaa
     2761 taaggcgacc ccgataccca ggctgatcag gggccagatg aggccaccgg ccattgcccg
     2821 ttgcgtgccg ggataatcac ctaccatttt gtttatttta ttaacctgta taaaataact
     2881 gataaggccc atgattgaat tgatgaggga gacgatggcg gcaagaccgg cccatactgc
     2941 catgtcgcga agccctgact ttacattatt atcaaaactc aggtcgaata atgaattgtt
     3001 gctggatgaa ttttccattt ttaatttttt gagttagaag ctggtggtta aggcagacaa
     3061 aaggctcagg atcgacagaa cgaaactgat aatgaaatag gtcttcatgg acctgaatcc
     3121 atccgccagc atggcggaat ccctgttctg aagtccgctg ctcagcaggt ttgatccttt
     3181 gaagagaaaa taaatccaga ggcccatgat cagcaaggcc acgatgacaa tggcgatcat
     3241 tgccccggcc atatccagcc ctccgaatcc tgaaaaagtg gataactgac ctgctaactg
     3301 ggatccggcc accagcaata agatcaatac cagtccgagc gagacaaaac cggtaattga
     3361 aataaagcgg gtccattttg ccactgactg caggtgttcc tgcgtgacat gatccatact
     3421 ggtgtcaaat aatgattgtt cctggttagg ttccatatac agttgattta gtcttaaata
     3481 taaaactccg gtctgtctac tgcaaatatt tttacaatta aaagaccgga gcaataaaaa
     3541 aaggatggtt gatcagtaag ctctcgcaaa aagcacccgt tcagcagaag gctttccgga
     3601 aaagatacag gttccctctt ccttcggatt atccaggggg atacaacgga ttgtagcttt
     3661 agtcttttct ttaatagcct cttctgtttc cggtgtcccg tcccaatgtg cggaaataaa
     3721 accggctttt tcatccagga gtttttcaaa ctcttcccaa ctgttggcgg gagtgatatt
     3781 ggcatcgcgg taggcttttg ccttatcaaa catgaattgc tggatttcca gcaacaggtt
     3841 gtctatatat cctgcgatcc catccagg
//
LOCUS       DAOPMW010000329         3862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28038_length_3862_cov_6.636459, whole genome shotgun sequence.
ACCESSION   DAOPMW010000329 DAOPMW010000000
VERSION     DAOPMW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28038_length_3862_cov_6.636459"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1112)
                     /locus_tag="PLU11_12900"
     CDS             complement(24..1112)
                     /locus_tag="PLU11_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPN60075.1"
                     /translation="MNDFLNEVWGGNPVRSYLIVAAVILFVWLFRSFISRYFAGLLFG
                     IVHRIWKNVDKPAFTSLVIKPMGFFLLILVSVAALYKLNFPEELNETIYRYSIKEILQ
                     SVGTVILIVSFFELLLRLIDFIALILEKKANLTPDQSDNQLIVFFKDFFKVILVIIGT
                     MLVLRFAFGLDVGGLLTGLSIVGAAIALALRESLENLIASFIIFFDKPFITGDLVKVN
                     AIIGTVERIGLRSTRIRTDQKTYVTVPNKQMVDSVLDNLSLRTQRRGDLRLDIGLQTP
                     PASIEQLLEGIRKIVGREEVEEYNVNLSEISSQAIVIVSDYYTAPIGIKEFFTIRQEI
                     NLAALKLMEELKIEVAGASTDVKLVSSR"
     gene            complement(1158..1991)
                     /locus_tag="PLU11_12905"
     CDS             complement(1158..1991)
                     /locus_tag="PLU11_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60076.1"
                     /translation="MKQLSGLILILFFAAFQGSAQTDTSFRLLRILKGDIVHFTIDNL
                     DNIYVLNNQNQVKKYNANGDSVAIFNDIRKYGRASLIDVSNPLKVLLYYRDFATVVML
                     DRFLNPVNTVDLRKENIFQAKTIAQSYDNKIWVYDEVENKLKKIGEEGKLIQETPDFR
                     LLLDQSVNPVKIADENQYVYLYDSTKGVYVFDYFGSLKNVIQIHGWKNLKVNGNYIFG
                     SLRDTLYRYEISSFLYDEWILPAELRSATVFNFSNSRLYALRPEADGKTTGIAIYTLR
                     R"
     gene            complement(1988..2773)
                     /locus_tag="PLU11_12910"
     CDS             complement(1988..2773)
                     /locus_tag="PLU11_12910"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPN60077.1"
                     /translation="MTITPEKKIYFLSDFHLGAPDHASSLERERKIVQFLDEIKDQAA
                     EIFFVGDMFDFWYEYRTVVPKGFVRLLGKIGELSDAGIPMHFFVGNHDMWMKNYFQQE
                     FNMPVYYAPEEFERNGKKILVGHGDGLGPGDHGYKRLKKVFRNPVSQFLFGIFPPMLG
                     MGLANYMSRRSRAQTGTKEEVFLGEDKEWLVIYCKEKLKQKKTDFFVFGHRHLPIDFR
                     LNESSRYINLGDWIHYFTYAVFDGVDMELKSYTGMEHKIIRKG"
     gene            complement(2806..3231)
                     /locus_tag="PLU11_12915"
     CDS             complement(2806..3231)
                     /locus_tag="PLU11_12915"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPN60078.1"
                     /translation="MTSHTNHNLFVYGSLRSGFQSHAYEYISRFFTLVGPAKVKGRLV
                     DMGAYPAGIPTDEAHYIIGELYQIKNANEFSWAMGQLDDYEGVNAEEEHERLYGRAIT
                     EVIAGDMPLEAWIYWYLGDTSGRPVIESGDILQYMAGKQ"
     gene            complement(3258..>3862)
                     /locus_tag="PLU11_12920"
     CDS             complement(3258..>3862)
                     /locus_tag="PLU11_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPN60079.1"
                     /translation="KKIRKALSHSTPLPFPQSEGNGTVYQPLQQEAEVEFAEQFTKLQ
                     GKFIFCINRQELAFQLGSLVRKQDWQKVYCVEEVLIDSLSAQIEDRLVKTDLAHCDVA
                     ITSCEYLVARTGSIVLSASTASGRSTSVYAPIHICIAYTSQVVYDLKDALQAVKDKYG
                     NNLPSLITFASGPSRTADIEKTLVVGVHGPKEVYLFLVEA"
BASE COUNT         1060 a         1010 c          806 g          986 t
ORIGIN      
        1 tgttgttgac tactaactac taactaccga ctactaacta gtttcacgtc cgtactcgct
       61 cccgccacct caatcttcaa ctcctccatc aacttcaacg ccgccaggtt aatctcctgg
      121 cgaatggtaa agaattcctt aataccaatc ggggcggtat aataatcaga gacgatcaca
      181 atagcctgac tgctgatctc ggagaggttg acattgtact cctccacttc ctctctgccc
      241 acgatcttac gaatgccttc cagtaattgc tcaattgagg ctggcggcgt ttgtaagccg
      301 atatcaagcc gcagatcacc ccgacgctgt gtacgcaagg agagattatc cagcaccgag
      361 tccaccatct gcttattggg taccgtaaca taggtcttct gatcagtacg gatccgcgtg
      421 ctgcgcaaac cgatccgctc caccgtacca ataatagcat tcacttttac caggtcaccg
      481 gtaataaaag gcttatcaaa gaaaataata aaggaagcaa tcaggttctc cagactctcc
      541 cgaagagcaa gggcaatggc cgcacctacg atactcagac cggtaagtaa ccctcccaca
      601 tcgagtccaa aagcaaaacg cagcaccagc atagtaccaa tgatcaccag gatcacttta
      661 aagaaatcct taaagaaaac aatcagctgg ttatcggact gatccggggt gaggttggcc
      721 tttttctcca ggatcagagc aatgaaatcg atcaggcgta acagcaattc aaaaaaggaa
      781 acaataagaa taacggtacc cacgctctgc aaaatttctt tgatactgta ccggtagatc
      841 gtctcattga gttcttcggg aaaattcagt ttatataaag cagccacaga taccaggatg
      901 agcaggaaga agcccatcgg ctttatcacc aggctggtaa acgccggctt atctacattc
      961 ttccatatgc ggtgtacaat cccgaataaa aggcccgcga aatagcggga aataaaggat
     1021 cgaaacagcc atacaaaaag gatcaccgcg gccactatca ggtaactgcg aaccggattc
     1081 ccgccccaca cttcattcag aaaatcattc atgataactt attaaaaagc aaaagtattg
     1141 aaacaacggg atatggatca gcgacgcagg gtgtaaatgg cgattcctgt tgttttccca
     1201 tctgcttccg gacgcagcgc atagagacgg ctgttgctga aattaaaaac agtggctgac
     1261 cggagctcag caggcaggat ccattcatca tacaaaaagc tgctgatctc gtaacgatac
     1321 aacgtatccc gcagggaacc gaaaatataa ttcccgttta ctttgaggtt tttccaacca
     1381 tgaatctgga tcacattttt caacgacccg aaataatcaa atacataaac gcctttggtg
     1441 gaatcataga gatagacata ctgattctca tcagctatct tcaccgggtt taccgactga
     1501 tccagtaata aacggaaatc cggtgtctcc tggatcagct tcccctcctc gccgatcttt
     1561 ttcagcttgt tctccacttc atcatacacc catattttat tatcataaga ctgggcaatc
     1621 gtttttgcct ggaaaatatt ctctttacgc agatccaccg tattcacggg attgagaaag
     1681 cggtcaagca tgaccacggt ggcaaagtcg cggtaataca ataatacctt gagcggattc
     1741 gacacatcga tcagactggc cctcccgtat ttccggatat cattgaaaat ggccaccgag
     1801 tccccgttgg cattgtactt tttcacctgg ttctggttgt tgagtacata gatattatcc
     1861 aggttgtcga tggtaaaatg gacaatatcc cctttcagga tgcgcagtaa ccggaaactc
     1921 gtatccgtct gtgcacttcc ctggaaagct gcaaaaaata aaatcaatat cagtccgctt
     1981 agctgcttca tcctttccgg ataattttat gttccatgcc cgtatacgat ttcagttcca
     2041 tatccacccc gtcaaacacg gcatacgtaa aataatgaat ccagtcgccg aggttgatat
     2101 accggctgct ttcattcaga cgaaaatcaa tcggtaaatg tctgtggccg aacacaaaga
     2161 aatccgtctt cttttgcttc agtttctctt tacagtaaat caccagccat tccttgtctt
     2221 cgcccaggaa tacttcctct tttgttcccg tctgtgcccg gctgcgtctg ctcatataat
     2281 tagccaatcc catgccaagc atcggtggaa atatcccaaa aagaaactgg ctgaccggat
     2341 tacggaaaac tttcttgagt cgtttgtagc catgatcccc gggaccgagc ccgtcaccat
     2401 gtcccaccag tatctttttc ccgttccgtt caaattcctc gggagcataa tacaccggca
     2461 tattgaattc ctgctgaaaa taatttttca tccacatatc atggttaccc acaaaaaaat
     2521 gcatggggat gccggcatcg gataattcac cgatttttcc gagtaaacgt acaaaaccct
     2581 tgggcaccac ggtgcggtac tcataccaga aatcaaacat atcgcccacg aaaaagatct
     2641 ccgccgcctg gtctttgatc tcatccagga actgcacgat tttccgttcc cgctccaggc
     2701 tgctggcatg atcgggggcg ccgagatgga agtcggagag gaagtagatt ttcttttcag
     2761 gagtgatcgt catgcgcgct gctaaaagaa ttttccggtt actgattact gtttgccggc
     2821 catatactga aggatatccc cggattcgat gaccggtcgg ccgctggtat cgccaaggta
     2881 ccagtagatc caggcctcca ggggcatatc gccggcgatc acttcggtta ttgcgcggcc
     2941 atagagtcgc tcgtgttcct cttccgcatt cacgccttca taatcatcca gctgacccat
     3001 ggcccaggaa aattcattcg catttttgat ctggtaaagt tccccgatga tataatgcgc
     3061 ttcatctgtc ggaattcctg ccgggtaggc acccatatcc accagcctgc cttttacttt
     3121 cgcaggaccc accagggtaa aaaaacggct gatatattca tacgcatggc tctggaatcc
     3181 actgcgcagg gagccgtaaa caaaaagatt atggttcgta tggctagtca tacctaaaag
     3241 tacaaaaggg agctgattta tgcttcaacc aggaataaat agacttcttt gggaccatgt
     3301 acacctacta ccagtgtttt ttctatatct gcagtacgac tgggaccgga agcaaaagtg
     3361 atcagtgagg gcaggttatt cccgtattta tcttttactg cctgcaaggc gtctttaaga
     3421 tcatacacca cctgactggt atacgcaata cagatatgaa tgggcgcata tacactggta
     3481 ctccggccgc tggcagtgga agcactcagc acaatcgatc ctgtacgggc caccaggtat
     3541 tcacaactgg taatggccac atcacaatgc gccaggtctg tttttaccag ccggtcctcg
     3601 atctgtgccg ataaagaatc aattaacacc tcctccacgc aatacacttt ctgccagtcc
     3661 tgtttgcgta ccaggctccc cagctgaaaa gccagttcct gccggttgat gcagaaaata
     3721 aacttgccct gcagtttggt aaactgctcg gcaaattcaa cttctgcttc ctgctgcagg
     3781 ggctgataca ccgtaccatt cccttcgctt tgcggaaaag gcaaaggcgt tgagtgactc
     3841 aacgccttgc ggattttttt aa
//
LOCUS       DAOPMW010000330         3851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28152_length_3851_cov_6.117229, whole genome shotgun sequence.
ACCESSION   DAOPMW010000330 DAOPMW010000000
VERSION     DAOPMW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28152_length_3851_cov_6.117229"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1243
                     /locus_tag="PLU11_12925"
     CDS             41..1243
                     /locus_tag="PLU11_12925"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPN60080.1"
                     /translation="MNKNLAKLWQAGQKSSRIILGLMSGTSFDGLDLALCRISGSGAE
                     TSVNLLNFTTVPYKPAFKESLKSIFSRRDADLENVCLLNSQVAIEHAQMINQVLRDWS
                     VKNEAVDLVASHGQTIYHAPAFLHGRPEFGNATLQIGDGDHIAVHTGIITISDFRQKH
                     VAAGGEGAPLAVYGDYLVFSKKLENRIMLNIGGIANFTYLPGDLDAGKVFSSDVGPGN
                     TMMDALMQSAYPGQYYDQDAGVARKGTVHPALLMALKEHAFFSGPFPKTIGPELFNLH
                     YLDKAIQSCGAGALPVEDQMATLNQLSADMIVDAIKRTVDMNSPFTVYVSGGGMHNPL
                     LIENIREQLKGIPFHSTQALEINPDAKEAVLFALLANETVAGGAVQLGENRKGIPNVS
                     MGKISFPD"
     gene            complement(1295..1792)
                     /locus_tag="PLU11_12930"
     CDS             complement(1295..1792)
                     /locus_tag="PLU11_12930"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN60081.1"
                     /translation="MKQILLLLSVFMITHTSNAQGGGKKSPHDTVSSKVTTVTYGRPY
                     KKGRVIFGELEKYGKVWRVGADEATTIKFASDVKFGGVDVKAGTYTLFAIPGETEWTL
                     ILNSQLGQWGAYGYEKNKDKDIASVKVGSQKLDMPVEQLTIRFIESGAMVIEWDATRV
                     LVTIN"
     gene            complement(1849..2349)
                     /locus_tag="PLU11_12935"
     CDS             complement(1849..2349)
                     /locus_tag="PLU11_12935"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN60082.1"
                     /translation="MRLIKLAFISFFVFFLLITGISLFFPSHVRISKAIDINAPRDSV
                     RAMISQPLHWKNWYPGADTLALYEADGVIKGIRMPGNQVLMITGFNDSTVMAANSSSG
                     TERASMGWNLIQSNPKTVTVQWYMDFKLRWYPWEKFSSLLLEKRYGPVMEQGLAKLKT
                     TVQATP"
     gene            complement(2410..3528)
                     /locus_tag="PLU11_12940"
     CDS             complement(2410..3528)
                     /locus_tag="PLU11_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN60083.1"
                     /translation="MSNVQKPYSQTRALWAITRASFKAIFGNPSAIIFSILFPIIFVL
                     IFGAFGGGGGVSYRIALAGKVDTADFFYKGLLQIPGVKIVAYTDSAVLRKDLIKGKLT
                     AILDIQKTTDSTEKPHYNVSIKSTSASSNTIYSFVPALKMVKLMLEKELNDSYVSYVD
                     IEEPVIEQVRQYRQIDFILPGQLGFSILFSTLFGIAFTFFNLREQLVLKRFYASPVKK
                     INILLGVGMSRLFFQVLSVLVLLLFGHFFMNFTLQNGIFTFFSIITMTLIMLFLLMGV
                     GLIFSSIVKSDTSIPLLINLFGFPQMMLSGTFFPIEVFPKWLQEICRLLPLTQYNDAV
                     RKISFEGLSLLECWKEIGILGIWMVIIYAIATKVLKWE"
     gene            3586..>3851
                     /gene="folK"
                     /locus_tag="PLU11_12945"
     CDS             3586..>3851
                     /gene="folK"
                     /locus_tag="PLU11_12945"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPN60084.1"
                     /translation="MNTAWLLTGSNMGIRENYLSAAKREIALQCGTVQKQSSIYETAA
                     WGKTDQPAFLNQALEIMTPLNARQLIRRLLKIEKQLGRIREEKYG"
BASE COUNT         1089 a          901 c          876 g          985 t
ORIGIN      
        1 taaatttttc cggcaaaaac cgacaatcta actttaaaac atgaacaaaa acctggcaaa
       61 actctggcaa gccggccaaa aatcttcccg tattatactt ggattgatga gtggtacatc
      121 gttcgatgga cttgacctgg cattatgccg gatcagtgga agtggtgccg aaacttcggt
      181 aaatctgttg aatttcacta cagtccccta caaacccgct tttaaagaat ccctgaaatc
      241 cattttttcc cgcagagatg ctgatctcga aaatgtgtgt ttgctcaaca gccaggtagc
      301 cattgaacat gcccaaatga ttaaccaggt tctgcgggat tggtctgtaa aaaatgaagc
      361 ggttgacctg gtggccagtc atggtcagac gatttaccat gcacccgctt ttttacatgg
      421 ccggccggag tttggaaatg ccaccctgca gattggcgac ggagatcata ttgccgtgca
      481 caccggcatc ataaccatca gtgatttcag gcaaaaacat gtggcagccg gcggagaagg
      541 tgcaccactg gccgtttatg gagattacct ggtgttttcg aaaaagttag aaaacaggat
      601 catgctgaat atcgggggga ttgccaactt cacttatctg cctggagacc tggatgccgg
      661 gaaagtattc agttcggatg tggggccggg taataccatg atggatgcat tgatgcaatc
      721 agcctatccg gggcaatact acgatcagga tgccggagtt gcccgtaaag gaaccgtgca
      781 cccggctttg ctgatggctt taaaagagca tgcatttttt agtgggcctt tccccaaaac
      841 aatcggaccg gaattattca atctccacta cctggataaa gccattcagt cctgtggggc
      901 aggagcactg ccagtggaag accagatggc taccttaaat caactcagtg ccgacatgat
      961 cgttgatgcg attaaaagga ctgtggatat gaatagcccg tttactgttt atgtgagtgg
     1021 tggcggaatg cataacccgc ttctgattga aaatatccgg gagcagctaa aaggaatacc
     1081 cttccattcc acccaggccc tggaaataaa tccggatgcc aaagaagccg ttttatttgc
     1141 cctgctggcc aatgaaacag tggccggcgg agctgtacag ctgggtgaaa acaggaaggg
     1201 tattccgaat gtatcgatgg ggaagattag ttttccggat tagtgtggca actggtaagg
     1261 ccacttagag tggccatacc agttgccaca aggactagtt aatcgttacc agtaccctcg
     1321 tcgcatccca ctcaatcacc atagcaccgg attcaataaa gcgaatcgtt agttgttcca
     1381 ccggcatatc cagtttctgt gatccaactt ttacggaagc aatatccttg tctttgttct
     1441 tctcataccc ataagcgccc cattgaccca actgtgaatt caggatgagt gtccattctg
     1501 tctcaccggg aatagcgaat aaagtatagg taccggcttt tacatccacg ccgccaaatt
     1561 tcacatccga agcaaacttt atggtagtgg cttcatcggc ccctacccgc cataccttac
     1621 catatttttc cagttcacca aaaatcaccc ggcctttctt atacggacgg ccataggtaa
     1681 cggtagtaac ttttgaactt acggtatcat gcggactttt ctttccacca ccctgtgcgt
     1741 ttgaagtgtg ggtgatcata aaaacggaaa ggagaaggag gatttgtttc atatgtttag
     1801 tttttgttta ctaattgttc tggccactga agacaactgt tctggccact acggagtggc
     1861 ctgaacagtt gtcttcaact tcgccagccc ctgctccatc accggaccgt atcgtttctc
     1921 cagcaaaaga ctggaaaatt tttcccaggg ataccagcgc agtttgaaat ccatatacca
     1981 ctgaactgtt acggtttttg gattgctctg tatcaggttc cagcccatac tggcccgttc
     2041 agttccactg ctgctgttgg ccgccattac cgtgctgtca ttaaaaccgg taatcatgag
     2101 cacctggttg cctggcatcc ggattccttt gatcactcca tctgcttcat agagcgccag
     2161 ggtatcggca ccaggatacc agtttttcca gtgaagcggc tgactgatca ttgcccgtac
     2221 cgaatccctg ggggcattga tatcaatcgc ttttgaaatc cggacatggg acgggaaaaa
     2281 aagggaaata ccggtgatca gcaaaaaaaa tacaaaaaaa cttataaagg ccagtttaat
     2341 gagtcgcata ccaaacagat ttctgcttat gcaggttaat aaaggtcaac aattttgggt
     2401 ccaaagggtt tactcccatt tcaggacttt agtcgcaatc gcataaataa ttaccatcca
     2461 gatacccagt atcccgattt ccttccagca ttccagcaaa ctcagtccct caaatgaaat
     2521 tttgcggacc gcatcattat attgggtcaa tggtaataac cggcagattt cctgtaacca
     2581 ttttgggaaa acttcaatcg gaaagaatgt tcccgataac atcatctggg gaaaaccgaa
     2641 gaggttgatc agcaagggaa tcgaggtatc actttttaca atactgctga aaatcaatcc
     2701 gactcccatc agcaggaaca gcatgataag ggtcatcgta atgatggaga agaaggtaaa
     2761 gattccgttt tgaagcgtga agttcataaa gaaatgaccg aacaggagca gcaccagcac
     2821 acttaatacc tggaaaaaca ggcggctcat gcccacgccg agcaggatat tgatcttttt
     2881 aaccggtgaa gcataaaaac gtttgagcac caattgttcc cgcaggttaa aaaaggtaaa
     2941 ggcgatacca aataaagtag agaacaaaat ggaaaagcct aactgtcccg gcaggatgaa
     3001 atcgatctgc cggtactgcc tgacttgctc aatcacgggt tcttcaatat cgacataact
     3061 gacataactg tcattcagtt ctttctccag catcagtttt accatcttca gagcaggcac
     3121 aaacgaatag atggtatttg aactggcgga agttgattta atacttacat tatagtgtgg
     3181 cttctccgtg ctatccgttg ttttttgaat atccaggata gcggtcagtt ttcctttaat
     3241 caggtctttt cttaataccg ctgagtctgt atatgctaca atttttacac cgggtatctg
     3301 tagtagtcct ttataaaaaa aatcagccgt atccacttta ccggccagcg caatccggta
     3361 actcacccca cccccgccgc caaaggcacc gaaaatcagc acaaaaatga ttgggaaaag
     3421 tatactgaat ataatggcac tcgggttgcc gaaaatagct ttaaaactgg cccgggtgat
     3481 ggcccataaa gcccgggtct ggctataagg tttttgaacg tttgacatgc tgggggcaaa
     3541 tgtatttaat tacggggcat cccggaaaat aaagatattt ggggcatgaa tacggcctgg
     3601 ttactgacgg gaagcaatat gggtatacgg gaaaactacc tttccgccgc aaaacgggag
     3661 atcgcgttac aatgtggaac ggttcaaaaa caatcctcca tttatgaaac ggccgcctgg
     3721 ggcaagaccg accaacctgc cttcctgaat caggcgctcg aaatcatgac cccgctcaat
     3781 gcccgtcaac tgatccgccg tttgctgaaa attgaaaaac aactgggcag gatccgggag
     3841 gaaaaatatg g
//
LOCUS       DAOPMW010000331         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28175_length_3849_cov_11.232472, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000331 DAOPMW010000000
VERSION     DAOPMW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28175_length_3849_cov_11.232472"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1076
                     /locus_tag="PLU11_12950"
     CDS             165..1076
                     /locus_tag="PLU11_12950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN60085.1"
                     /translation="MVSVIVPSYNYGHLIADTIASIRKQSDPDWEMIIVDDGSTDDTE
                     TVVRSLMQEDPRIRFFRQQNAGPSAARNLGLREAKGNFIQFLDADDLLQPEKFALQLA
                     VFASSPETDVVYGPVRYFTKDPADESALLYTYWGVNKEWMPKISGQGKDLLVPALNGS
                     FAHISCFLFRKSVVDKAGPWDTTKRAAEDYLFVLNCVLEGACFAYIDRKGSYALVRWH
                     DSNASRDVKWIHEGERQMRIELAPRIEMTGNAAAIETNRNAIKALAMMNQHSWRKFFL
                     SGGPLDFLKKGLRWLGLEKWARKIFYK"
     gene            1109..>1661
                     /locus_tag="PLU11_12955"
     CDS             1109..>1661
                     /locus_tag="PLU11_12955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60086.1"
                     /translation="MKVLFDTQVFDWQINGGISRYFIEVLNRLRQDQEVEVLFKCRHS
                     YNTYIQGTPWLTNRPVLKKLHFKGKLGALKIINQQINRPWSNRQLSKEKAAIFHPTYY
                     DPYFLQRLGKQKMVLTVYDLTNEKFNDHTPLTEKVLGWKKQLIERADHIIAISENTRK
                     DVIDYYHADPDKVTTVYLSGGFDA"
     assembly_gap    1662..1761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1762..2055
                     /locus_tag="PLU11_12960"
     CDS             <1762..2055
                     /locus_tag="PLU11_12960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60087.1"
                     /translation="FVFPSLYEGFGLPVLEAMQCGCPTLLSNNSSLPEVGGGAGAYFD
                     PQQPGALTPVLDELLRDETRRQQMIAAGYQQATRFNWDRTAEGHKEVYKKLLR"
     gene            2052..3029
                     /locus_tag="PLU11_12965"
     CDS             2052..3029
                     /locus_tag="PLU11_12965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN60088.1"
                     /translation="MKKAIISGISGQDGAYLARLLVQQGYQVTGLVRGSQGEGLWRLK
                     YLGIAEQVQLTECDLTDMSQIFRLIQEIQPDEVYHLAAQSSVSVSFSQPIATINYNTI
                     STLNLLETIRLSHASLRFYQASSSEMFGAVQRLPIDEDTVINPQSPYAISKATGYWMT
                     RNYREAYKLFATSGILFNHESALRQPNFVIKKIIRTALDIREGKADKLVLGNIDVRRD
                     FGYAPAYVEAMWRIMQQDQPDDFLICSGFSSSIREIVEMVFVLLEVPLSALHTEQSLF
                     RPAEIDENFGNPEKAKRVLNWSYSMNLEQLLRQLILDEYEVREFLKLHS"
     gene            3061..>3849
                     /locus_tag="PLU11_12970"
     CDS             3061..>3849
                     /locus_tag="PLU11_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN60089.1"
                     /translation="MNAPRISIITPSFNQGDFIEETILSVLNQDYPNLEYIIIDGGST
                     DQTVDIIKKYSDRISYWVSEKDRGQSHAINKGFERAKGEIINWINSDDQLMPGTLHKL
                     AALFNVDPELMMVHGRIEYFGETRPYYSVNLPAKDLDTRYISHICMPQPATFYRRQLL
                     LEQGLLDESLHFSMDTDLFVRAGLHYKIHQSDEVFARFRLHSASKSVSAFNKNFLKDN
                     AVIFSKTITSLGLKQAIAEMKQLDLYTAPSELYKPSSRTFDGNKM"
BASE COUNT         1016 a          893 c          894 g          946 t
ORIGIN      
        1 cacctggcag aaaacctggt tttattggtg ttgctgtcct ggttgtcatt tgtattaatt
       61 gaaaaacgtt ttctgggtct gaaaaaatat ttcgaatata gtcctgagcg ccccgtggca
      121 acggcaaaat gatccgggat tacggtacct cttttcatcc taccatggtt tctgtaatag
      181 tcccttctta taattatggc cacctgattg cggataccat tgcctccatc cggaagcaat
      241 ctgatccgga ttgggaaatg atcattgtgg atgacggatc cactgatgat accgaaacgg
      301 tggtacggtc actgatgcag gaagacccgc ggattcgttt ctttcgccag caaaatgcgg
      361 gacccagtgc cgcccggaac cttggtcttc gggaagccaa aggaaatttc attcagttcc
      421 tcgatgcgga tgacctgctg cagccggaaa aatttgcact tcagctcgcc gtttttgcgt
      481 ccagcccgga gacggatgtg gtctacggac ccgtccggta ttttacaaag gaccccgccg
      541 atgaatcggc attattatat acctactggg gagtgaacaa ggaatggatg ccaaaaatca
      601 gcgggcaggg taaggattta ttagtgcccg ctttaaatgg cagttttgcc catatcagtt
      661 gttttctttt ccgcaaatcc gtcgtggaca aagcgggccc ctgggatact accaagcggg
      721 cggctgaaga ttatctgttt gtattaaact gtgtgctgga aggcgcctgt tttgcttaca
      781 tagaccggaa gggcagctat gccctcgtgc gctggcacga ttccaatgcc agccgggatg
      841 taaaatggat tcatgaggga gaacgacaga tgcggatcga actggcaccc cggatcgaaa
      901 tgaccggtaa cgccgccgcc atcgaaacga accggaatgc cattaaagcc ctcgccatga
      961 tgaatcagca ctcctggcgg aaattttttc tcagcggcgg cccccttgat tttttgaaaa
     1021 agggattgcg ctggctgggc ctggaaaaat gggccagaaa gattttttat aaataactgt
     1081 atccacacga ctttaaaaat cagcagcatt gaaagttctt tttgatacac aggtattcga
     1141 ctggcagatc aatggcggca tttcccgcta ttttatcgag gtactgaacc ggctccggca
     1201 ggatcaggaa gtggaagtgc tttttaaatg ccgtcattct tacaatactt atattcaggg
     1261 tacgccctgg ctgaccaacc ggccggtact gaaaaaatta catttcaaag gcaaactggg
     1321 cgccctcaaa attattaacc agcagatcaa ccggccctgg agcaaccggc agctgagtaa
     1381 agaaaaagcg gccatttttc atcctaccta ttatgatcct tattttttac aaagattggg
     1441 aaaacaaaaa atggtactga cggtctatga cctgaccaat gaaaaattta atgaccatac
     1501 gccgctgacc gaaaaagtgc tgggctggaa aaagcaatta atcgaaagag ctgatcatat
     1561 cattgctatc tcggaaaata cccggaaaga cgtaattgat tattatcacg cggatccgga
     1621 taaagtaacc acggtttacc tgtctggtgg attcgacgcc gnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn ntttgtattt ccatccctct atgaaggatt cggactgccg
     1801 gtactcgaag ccatgcaatg cgggtgcccc acattgctga gcaacaattc ctccctgccg
     1861 gaagtggggg gaggagccgg tgcttatttt gatccgcagc aaccgggtgc tttaaccccg
     1921 gtgctggatg aactgctccg cgatgaaacc cgccggcaac aaatgatcgc cgcaggttac
     1981 cagcaggcaa cccgctttaa ctgggatcgt actgccgaag ggcataaaga agtatataaa
     2041 aaactgctcc gatgaaaaaa gcaattatat ccggaatatc cggacaggac ggtgcctatc
     2101 tcgcccgctt attggtgcag cagggttacc aggttaccgg actggttcgt ggcagccagg
     2161 gcgaaggact gtggcggttg aaatacctgg gtattgctga acaggttcaa ttgacggaat
     2221 gtgacctcac ggatatgtcc cagatattcc ggctgatcca ggaaatacaa ccggatgaag
     2281 tctatcacct ggcggcacaa agctccgtgt cggtttcttt ctcacaaccg attgccacga
     2341 ttaattacaa tacgatttcc acccttaact tgctggagac catccggctt tcgcatgcat
     2401 ccctccgctt ttaccaggcg tccagcagcg aaatgtttgg ggcggtacaa cggttaccga
     2461 ttgatgagga tacggtaatc aatccgcaaa gtccctatgc catttccaaa gccaccggct
     2521 actggatgac ccgcaattac cgggaagcct acaaactgtt tgccaccagt ggtattttat
     2581 tcaatcatga atcagccctg cgtcagccca attttgtgat aaagaagatt atcagaacgg
     2641 ccctggatat ccgggagggc aaggccgata aactggtatt gggaaatatt gatgtccgcc
     2701 gcgatttcgg ttatgctccg gcttatgtgg aagcgatgtg gcgtatcatg caacaggatc
     2761 aaccggatga tttcctgatc tgttccggat tctcgtcctc catccgtgaa atagtggaaa
     2821 tggtatttgt cctcctcgaa gtacccctca gtgccttaca taccgagcaa agtcttttca
     2881 gaccggcaga aattgatgaa aacttcggca atccggaaaa agcgaaaaga gtgctgaact
     2941 ggagctatag tatgaatctc gaacaattat taagacaact tatattggat gaatatgagg
     3001 taagggagtt tttaaaatta cacagctgat tttctcgctt tttttaatat cactttttta
     3061 atgaacgctc cccgcatatc aataattact ccttccttca atcagggaga ttttatcgaa
     3121 gagaccattc tttccgtatt gaaccaggat tatcccaacc tggaatacat catcattgat
     3181 ggaggcagta ccgaccagac tgttgatatc ataaagaaat acagtgaccg gatcagttac
     3241 tgggtatccg aaaaagaccg gggacaaagc catgcgatca ataaagggtt tgaaagggcc
     3301 aaaggcgaga tcatcaactg gatcaacagc gacgatcagc tgatgccggg caccctgcat
     3361 aaactggcgg ccctgtttaa tgttgaccct gaactgatga tggtccatgg acggattgaa
     3421 tattttggtg aaacccgtcc ctattattcg gtgaacctgc ctgccaaaga cctggatacc
     3481 cgctacatct cccatatctg tatgccccag ccggctacat tctaccggcg ccaactcctc
     3541 ctggaacaag ggctgctcga tgaatccctg catttttcca tggatacaga tctttttgtc
     3601 cgggcagggt tgcattataa aatccaccag agcgatgaag tgtttgcccg tttccgcctt
     3661 cattcggcca gtaaatcggt cagcgcattc aataaaaatt ttctgaaaga caatgcggtt
     3721 attttcagca aaaccataac cagccttgga ttaaaacagg ccattgctga aatgaaacaa
     3781 ctggacctgt atacagcacc cagtgaattg tataagcctt ccagccggac ctttgatggg
     3841 aataaaatg
//
LOCUS       DAOPMW010000332         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28229_length_3845_cov_4.095251, whole genome shotgun sequence.
ACCESSION   DAOPMW010000332 DAOPMW010000000
VERSION     DAOPMW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28229_length_3845_cov_4.095251"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="lepA"
                     /locus_tag="PLU11_12975"
     CDS             <1..1137
                     /gene="lepA"
                     /locus_tag="PLU11_12975"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPN60090.1"
                     /translation="NDKVKFVSTGQEYGADEIGILKLKMTEKNVVKAGDVGYIITGIK
                     NAKEVKVGDTITVASNTNPQQIQGFEEVKPMVFAGIFPVNTDEFEELRDCMDKLQLND
                     ASLTYELETSQALGFGFRCGFLGMLHMEIIQERLEREFNQTVITTVPNVSFIAYTTKG
                     EKVIVNNPTEFPDPVRIDHIDEPFIKAQIISKPEYIGNIMTLCLGKRGILMNQSYLTP
                     TRVELLFEMPLTEIVFDFYDKLKSQTRGYASFDYHPIGYRESDICKMDILLNADKVDA
                     LSALIHRSRAQDFGRKLCEKLKELLPRQQFQIAIQAAIGAKVVARENISAMRKDVTAK
                     CYGGDISRKRKLLEKQKEGKKRMRQIGNVEVPQEAFLAVLKLDD"
     gene            complement(1180..1830)
                     /locus_tag="PLU11_12980"
     CDS             complement(1180..1830)
                     /locus_tag="PLU11_12980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN60091.1"
                     /translation="MTGRIREWLKRKFPVRNSRRIFGSIYRDREWDTGETLSGPGSTI
                     RYTEELRITLPALLKKWKIRSLLDAPCGDYHWMSQTKLDGIEYTGADVVAGLIESNRQ
                     LYPDVKFLIADITKDWLPKTDAVLCRDCFIHLSNKQVMMAIQQFKKSGIRYMIASTYP
                     VEINEEIPTGYYRPVNLVKDPFKLPAPVDCIKDYPDGEVERYLGVWDLFNQLVVSS"
     gene            complement(1827..2936)
                     /locus_tag="PLU11_12985"
     CDS             complement(1827..2936)
                     /locus_tag="PLU11_12985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN60092.1"
                     /translation="MKGKKIIFICSRLDTPGGIERAVINTANLFVQKGHSVTLLVADV
                     TGNTRCCYPLHPAVQVDFRSYSFGIGIRGNAISRKIRLYQDIKALRMYLKNQAADLLI
                     TTEYPFSIAAVLSGAGKKSRIIAWEHQHFNWIKKNKFWRWLQENFYPRLERMVCLNET
                     EAAHYSRYTPVTVIPNFIEQVYKDNQSYHKKQLLTVGWLIHRKGTDLLLPIAKTILNK
                     FPDISWKLVGDGELKELVQDFIRNENLGGRLLLQSPGETDIDAVYRESGLFVLPSRNE
                     AFPMVLLEALSYGIPCISFNCPSGPSAIITQAEDGWLVEPENTARLSEAIIQLLNEPE
                     RRQQMSVNALKNIQRFDKEKIYPLWATLINNTNQK"
     gene            complement(2936..>3845)
                     /locus_tag="PLU11_12990"
     CDS             complement(2936..>3845)
                     /locus_tag="PLU11_12990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60093.1"
                     /translation="IDLQYPMQPNDHLTVIKKLEALSFKYADAIFAHSPMNQAELKAL
                     DYPQAFFCRAPWILPQQTTGLRKPRKNSFLVISSLQMIKGAELMTQAAALAGKSMDAL
                     MVYWVGEDSYSAPGGQLTSAYLQQKYPDSWQQQLVWLGPKNREEINELIREASAVIIP
                     SLWDTFNYVVPETIYSGTPLILSDKTGASYLAQDYPTAILFPAGDVAALADKLISFDE
                     LAKKGSTSTDLTLENYFNPEAITAERMKLYATVIKTKSNPVNAEIRQALQFLDEYLTP
                     GRKIYYRLRKKIKALIHPDFNKLRHK"
BASE COUNT         1017 a          871 c          893 g         1064 t
ORIGIN      
        1 aacgataaag taaaatttgt aagtaccggc caggaatacg gggccgacga gattggtatc
       61 ctcaaactta agatgacgga gaagaatgtg gtgaaggcgg gcgatgtggg ttatatcatc
      121 accggtatca aaaacgcgaa agaagtaaaa gtaggggata ccatcacagt ggcctccaat
      181 accaatccgc agcagatcca ggggtttgaa gaagtgaagc cgatggtatt tgccggtatc
      241 ttcccggtga atacagatga gtttgaggaa ctgcgtgact gtatggataa actgcagttg
      301 aatgacgctt ccctgactta tgaactggaa acctcccagg cacttggttt tggtttccgt
      361 tgcggtttcc tcggtatgct gcacatggag atcatccagg aacgactgga gcgggaattc
      421 aatcagaccg tgatcaccac ggttcccaac gttagtttca ttgcctacac taccaaagga
      481 gaaaaagtga tcgtgaataa cccgacagaa tttccggatc ctgtacggat tgatcatatt
      541 gacgagccct ttatcaaagc acagatcatc tccaagccgg aatacattgg aaatatcatg
      601 accctttgcc tgggtaagcg gggtattctg atgaaccaga gttacctgac tccgacccgg
      661 gtggagctgc tgtttgaaat gccgctcact gaaatagtat tcgattttta cgataaactg
      721 aaatcacaga cgcgtggtta tgcttctttc gattatcatc cgattggcta ccgcgaaagt
      781 gatatctgta aaatggatat cctgctgaat gcggataaag tagatgcctt gtctgccctg
      841 attcaccgca gccgggccca ggatttcggc cgcaagcttt gcgaaaaact gaaagagtta
      901 ttgccccgtc agcaattcca gattgctata caagcggcta tcggcgccaa agtggtggcc
      961 cgcgaaaaca tctcggccat gcggaaagat gttaccgcca aatgttatgg aggggatatc
     1021 agccgtaaac gtaaactcct cgagaaacag aaagaaggaa agaaaagaat gagacagatc
     1081 ggtaatgtgg aagtaccaca ggaagcgttt ctggcggtgc tgaaactgga tgattgagga
     1141 agatgttttt tcttagaagt ggctgttgac tactatctac taactactaa ctactaactg
     1201 attgaagagg tcccaaactc ccaggtaacg ttccacctcc ccgtcgggat aatctttaat
     1261 gcaatcaaca ggggcgggta atttgaaagg atcttttacg aggtttaccg gtcgataata
     1321 accggttgga atttcttcat tgatttcaac gggataggta ctggcaatca tatacctgat
     1381 cccgcttttt ttaaactgct gaatcgccat catcacctgt ttgttcgaca aatgaataaa
     1441 gcaatcccgg caaagcacgg cgtccgtctt cggtaaccaa tctttggtaa tatccgcaat
     1501 cagaaatttg acatcagggt ataactgccg gttggactca atcagtccgg ctaccacatc
     1561 cgcacctgta tattcaatgc catccagttt tgtctgactc atccagtgat aatcaccgca
     1621 gggggcatcc agcagactcc tgatcttcca tttttttagt aaggcgggta aagttatacg
     1681 gagttcctct gtatatcgga tcgtggagcc tgggcctgaa agtgtttccc ctgtatccca
     1741 ttcgcggtca cggtaaatgg aaccgaagat ccgccggctg ttacgaaccg ggaatttcct
     1801 tttcaaccat tcacgtatac gtccggtcat ttctggttag tgttgtttat cagggtagcc
     1861 cacaacgggt aaattttttc cttatcaaaa cgttggatat tcttcagtgc attgacggac
     1921 atctgttgac gccgctccgg ttcattcagc aattgaataa tggcttcgct taaacgggct
     1981 gtattttccg gttcaaccag ccatccgtcc tctgcttgcg taataatggc tgacggaccg
     2041 gatggacaat tgaaactgat acagggtata ccatacgaga gcgcttccag taagaccatt
     2101 ggaaaagctt cattgcggga ggggagtaca aataaaccgg actcccggta tacagcatct
     2161 atatctgttt ctccgggaga ctgaagcagt aaccggcccc cgaggttttc attccggata
     2221 aaatcctgta ccagttcttt taattctcca tcgccgacca gtttccaact gatatccgga
     2281 aatttattta aaatggtttt agctatcggc aataacaagt cagtcccttt gcggtggatc
     2341 agccacccga cagtcagtag ttgtttttta tggtatgatt ggttgtcttt atatacctgt
     2401 tcaataaaat tcggaattac ggtaacgggt gtataccggc tataatgcgc cgcttctgtt
     2461 tcattcaggc ataccatccg ttccagccgg ggatagaaat tttcctgtaa ccagcgccag
     2521 aatttatttt tcttaatcca gttaaaatgc tgatgttccc aggcaataat gcggcttttt
     2581 tttccggctc cgctaagcac ggccgctatt gaaaacggat attcagtagt gatcagcaga
     2641 tccgcagcct ggttttttaa atacatcctt aacgccttta tatcctggta caggcggatt
     2701 ttccggctga ttgcattgcc cctgatacct atcccaaaac tgtaagaccg gaaatcaacc
     2761 tgaacagccg ggtgaagcgg ataacaacaa cgggtattgc ctgtaacgtc tgctacaagt
     2821 agtgttactg aatgtccttt ttgaacaaac agattcgccg tattgatcac cgctctttca
     2881 ataccgccgg gtgtatccag tcgggaacaa ataaatataa ttttttttcc cttcattact
     2941 tgtgcctcaa tttattaaaa tccggatgga tcagtgcctt tatttttttt ctaagccggt
     3001 aataaatttt cctgccggga gtgaggtact catccaggaa ttgaagggct tgcctgattt
     3061 cagcatttac cgggtttgac tttgtcttta ttaccgtagc atataatttc atcctttcag
     3121 cagtaatggc ttccggattg aaatagtttt caagtgtaag atccgtactc gtgctccctt
     3181 tttttgcaag ttcatcaaag ctgatcagtt tgtctgccaa agcagctaca tccccggctg
     3241 ggaataaaat agcagttgga taatcctgtg ccaggtagga cgctcctgtt ttgtcggaaa
     3301 gaatcaatgg agtaccggaa taaatggttt caggaaccac atagttgaat gtatcccaca
     3361 aggagggaat tataacagcg gatgcctccc tgataagttc attgatttct tccctgtttt
     3421 tgggaccgag ccagaccaac tgctgctgcc agctgtccgg atatttttgc tgcagatagg
     3481 cggaagtcag ttgaccaccg ggggctgaat agctgtcttc acctacccaa taaaccataa
     3541 gagcatccat ggatttgccg gccaaggcag cggcctgagt catcagttcc gctcctttga
     3601 tcatctgcaa actgctgatg actaaaaaac tattcttacg gggttttctt aacccggttg
     3661 tttgttgagg caatatccag ggagcccggc agaaaaaggc ctgcggataa tccaaggctt
     3721 ttaattcagc ctggttcatc ggactatgcg caaaaatggc atccgcgtat ttgaaagaca
     3781 gggcttccag ttttttgatg accgtcaggt ggtcattcgg ctgcatggga tactgcaggt
     3841 cgatc
//
LOCUS       DAOPMW010000333         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28246_length_3843_cov_5.370908, whole genome shotgun sequence.
ACCESSION   DAOPMW010000333 DAOPMW010000000
VERSION     DAOPMW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28246_length_3843_cov_5.370908"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLU11_12995"
     CDS             complement(<1..1467)
                     /locus_tag="PLU11_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN60094.1"
                     /translation="MQTHSKYKFITWLRQRNQQRKRTILLTGLMLLLLFLAKLSTAQD
                     SLSASTLSASQVMSIVKRYHPVARIADINIDMAKADITIARGQFDPQLYSYNAQKTFD
                     GTDYYNYNRPELNIPTWFGVSISTGLEYLSGSRTDPQETSGKTSYFGVTVPLAKNLLM
                     DKRRAALQTAKIYKDASQVEKNVIANNILQESIAAYWNWTLQYQVIQVLNEAVEVNRK
                     RVEFIKTGFRLGDRPGIDTIEAIAQLQNMELLQYQAILDFNNAGLGLSVYLWTSEQQP
                     YTLPSTVIPAPQPGLTELAVPVLDSLLNAARILHPELRLYDYKLDALAVEKKLKFQEL
                     LPTVNFTYNQLGKGYDVLKTATGPLFENNFRYGFSVGIPLRFSAGRGEYKKARLKIME
                     AETAQRYKQLQVETKVRAYYNELVTLRQQLLLQENNYKNYQLLLQGEETRFKNGESSL
                     FLVNTRENKTLEALQKLQELRNKYVKTFNSLQWAAGQLL"
     gene            complement(1471..2787)
                     /locus_tag="PLU11_13000"
     CDS             complement(1471..2787)
                     /locus_tag="PLU11_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPN60095.1"
                     /translation="MNNNEHPQWSSFRKVYRYNKRSRVRYWFYGLMTAMAIILFLPWT
                     QNIRAKGTVTTLRQEQRPQQLNTIIPGRVIKWYVKEGDYVQAGDTILQLAEIKDDYLD
                     PQLLSRTKEQLSAKSLTVDYYKNKVGTSGTQINALNEALQLKMDQLRNKLQQLQLKVQ
                     SDSMESIAASNDFKIATQQFTRQKAMYDSGLVSLTQLEQRNQTYQNSLAKKVSAENKW
                     QNARQDLTITRIEMNATQQDYMEKIAKAQGDQFQSLSQIATGQGDIAKLENQYSNYSI
                     RNGLYFITAPQSGQITKARKAGIGEIVKDSEMIVEIVPDQVQYAVELFVRPVDLPLIS
                     AGQEVRFMFDGFPAIVFSGWPNTSYGTFGGKVVAVESSVSYNGRFRILITEDPADKPW
                     PRELKMGTGAQGMALLKDVPIWYELWRNVNGFPPDFYKAGAEPEKK"
     gene            complement(2780..>3843)
                     /locus_tag="PLU11_13005"
     CDS             complement(2780..>3843)
                     /locus_tag="PLU11_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN60096.1"
                     /translation="IKSFKFSKGSVINMQKTDEKVSGYLDARNSHFSILQFQFKVLIA
                     FKVLITASMLIIGSLLLVNQQLNIGQFIAAEIVIISIIASVEKLIGNLDSVYDVLTSV
                     DKIAKITDKPVETSGSYILPEKREGLSVEAEKVSFAYDDEKYVLKDASFRIDAGETVC
                     ITGKEGSGKSTLLKLVSGSYQGFEGSLLLNRLPVGNYELKSLREQTGILLNQQDIFHG
                     SLLENITMGQPGIDLQQVIRLFGEVGLANFLGTLPQGFDTWLDPTGKRLSRNVVNKIL
                     LVRALVGQPRLLLLEEPWTHIEEPFRSRIIRLLLNDYPHTTILVVSADPEFTSRCSKV
                     IDVNCDGCIVKTNKSSNHE"
BASE COUNT          913 a          930 c          879 g         1121 t
ORIGIN      
        1 caatagctga ccggccgccc actgcaggct gttgaaggtc ttgacatatt tgttgcgcaa
       61 ctcctgtaat ttctgcaggg cttcgagggt tttgttttcc cgggtattga ccaggaacag
      121 cgaactctcc ccgtttttga aacgggtctc ttcgccctgc aacagcagct ggtaattttt
      181 ataattgttt tcctgcagga gcagctgctg acggagggtc accagttcgt tatagtaagc
      241 gcgtactttc gtctccacct gcagttgctt ataacgttgt gctgtttctg cttccataat
      301 tttcagcctg gctttcttgt attctccccg gcccgccgaa aaacgaaggg ggatacctac
      361 agaaaaacca tacctgaaat tattttcaaa gagcggtcct gtagctgttt tcaacacatc
      421 ataccctttc cccagctggt tatacgtaaa gttgactgtg ggcagtaatt cctggaactt
      481 taatttcttt tctaccgcca atgcgtccag tttataatca tagagccgca gttccgggtg
      541 tagaatacgg gctgcattca ggagactgtc cagtacagga accgccagct cggttaatcc
      601 cggttgtggg gctggaataa cggttgaagg cagggtatag ggctgttgtt ccgacgtcca
      661 gagataaacg gataatccaa gtccggcatt attaaaatca agaatggcct ggtattgcag
      721 cagttccata ttctgcaact gggcaatcgc ttcgatagta tcaatacctg gccggtcacc
      781 cagccggaaa cccgttttaa taaactccac tcttttgcgg ttcacttcca ccgcctcatt
      841 caatacctgg attacctggt attgaagtgt ccagttccaa taagcggcaa tggattcctg
      901 gagaatattg tttgcgataa catttttttc cacctgcgag gcatctttgt agatcttcgc
      961 tgtttgtagt gcagccctgc gtttatccat caacaggttt ttggccagcg gaacggtgac
     1021 tccgaaataa ctggttttac ccgatgtttc ctgcggatcg gtccggctgc ccgataaata
     1081 ttcgagcccg gtgctgatac tcaccccgaa ccaggtgggt atattcaatt caggccggtt
     1141 ataattgtaa taatcagtcc catcaaaggt tttttgtgca ttgtaactgt acagttgcgg
     1201 atcaaattgc ccacgggcaa tggtgatatc cgctttggcc atatcaatat taatatcggc
     1261 aatccgggct accgggtggt atcgtttaac gatggacatt acctggctgg ctgacagggt
     1321 tgacgcactc aggctatcct gtgcggtgga aagtttggca agaaagagaa gtaacaacat
     1381 cagtccggtt aagagtatcg tcctttttcg ttgttggttc ctttgccgca gccaggttat
     1441 aaatttatat tttgaatgtg tttgcatagg ttattttttt tcaggttcag caccagcttt
     1501 ataaaaatcc ggcgggaagc cattaacatt ccgccacagt tcataccaga taggcacatc
     1561 tttcagcaag gccattccct gggcaccggt gcccattttc aattcacgcg gccagggttt
     1621 atcagccggg tcttcggtaa taagaatgcg aaaacggcca ttataactca ccgaactttc
     1681 cacggccacc actttgcccc cgaatgtgcc atacgatgta ttgggccatc cgctgaagac
     1741 aatggccgga aatccatcga acataaaccg cacttcttgt ccggcagata tgagggggag
     1801 atccaccggg cggacaaaaa gctccacggc atactgcacc tggtcaggca cgatctccac
     1861 aatcatttca ctgtccttta caatttcacc aataccggct ttacgggcct tcgtgatctg
     1921 gccactttgg ggtgctgtga taaaatataa accattgcgg atggaataat tactgtactg
     1981 gttttccagt ttagcaatat ctccttgtcc ggtggctatc tgtgaaagcg actgaaactg
     2041 gtcaccctgc gccttggcaa ttttctccat ataatcctgc tgggtagcat tcatctcaat
     2101 tcttgtaatt gtcaggtcct gccgggcatt ctgccattta ttttccgcgc ttactttttt
     2161 agccaggctg ttctgatagg tttggttacg ttgctccagt tgggtaagtg agacgagtcc
     2221 gctgtcgtac atggcttttt gccgggtaaa ctgctgggtg gcgattttaa aatcatttga
     2281 tgctgcaatg gactccatac tgtcactctg cacttttaat tgtaattgct gcagtttatt
     2341 ccggagctga tccattttca gctgcagggc ttcattcaag gcattgatct gggtaccgga
     2401 agtgcctact ttgtttttat agtaatctac ggtcagggat ttcgccgata actgttcttt
     2461 ggtccggcta agcaattgcg gatccaggta atcgtccttg atctcggcca gttgaaggat
     2521 tgtatccccg gcctgtacat aatccccttc tttcacatac catttgatca cccgtcccgg
     2581 gataatggtg ttcagttgct ggggccgttg ttcctgccgt agagtggtca cggttccctt
     2641 tgcccgtata ttctgtgtcc acggaagaaa gagaatgatg gccatagccg tcattaatcc
     2701 gtaaaaccag tagcgcaccc ggcttctttt attatagcgg tataccttgc ggaaggagga
     2761 ccactgcggg tgttcattat tattcatggt tgcttgattt attcgttttg acaatacagc
     2821 catcgcagtt tacatctatt actttgctgc agcgactggt aaattcaggg tcagcgctaa
     2881 ccaccaggat ggtggtatgc ggataatcat tcaacagaag ccggattata cgactgcgga
     2941 aaggttcttc aatatgtgtc cagggttctt ccagcagaag caatcggggc tgacctacca
     3001 gggccctgac cagcaaaatt ttattcacaa catttctgga caatcgttta ccggttggat
     3061 ccagccaggt atcaaacccc tgcggcaaag tgcccaggaa attggccagc ccgacttccc
     3121 caaacaaacg gatgacctgt tgcaaatcaa ttccgggttg ccccatggtg atattttcca
     3181 gcaggctgcc atggaatata tcctgctgat taagcagaat accggtttgt tcacgcaatg
     3241 atttcaattc atagttgcca accggcaatc tgttcagcaa taaagaacct tcaaaaccct
     3301 ggtaagagcc gcttaccagt ttgagcaggg tggatttgcc gcttccttct tttccggtaa
     3361 tacaaaccgt ttcgccggca tcaatccgga aactggcatc tttcagtaca tatttttcat
     3421 cgtcataggc gaaactcact ttttcggctt caacagataa tccctctctt ttttccggaa
     3481 ggatatagct gccggatgtc tctaccggtt tgtccgtgat cttggctatt ttatctaccg
     3541 aggtcagtac atcatataca ctgtccagat ttccgatcag cttttccacc gaagcgataa
     3601 tggaaatgat tacgatctcg gctgcaataa actgcccgat attcagctgc tgattgacca
     3661 ataaaaggga tccgataatc agcatggatg ccgtgatcag cactttaaag gcgatcagca
     3721 ctttgaactg gaattgcagg atactgaaat gactgttccg ggcatccagg tagccactga
     3781 ctttctcatc tgttttctgc atattaataa ccgaaccctt ggagaatttg aaggatttga
     3841 tca
//
LOCUS       DAOPMW010000334         3828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28391_length_3828_cov_10.026769, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000334 DAOPMW010000000
VERSION     DAOPMW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28391_length_3828_cov_10.026769"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1205)
                     /locus_tag="PLU11_13010"
     CDS             complement(33..1205)
                     /locus_tag="PLU11_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN60097.1"
                     /translation="MSDTISREKFLKTTALAGAALLVSSLEGWAASLPDKKLRVAVIG
                     CGSVSNRYIPQLLSSKWIEVVSLCDIKPERAVAQNKEYKVNAATYGHIDEMLKGVPFD
                     MMVTLTDMQVHGELNKKALMAGKHVWSEKPMANTYAEGKALLDLAKTKKLRIWGAPAV
                     VNSPQFAYMSKMIQEGKLGRVAAAHGQYGHTGPTWSAFFYEKNGGSMPDLGVYNIATL
                     TGLLGPARSIMAMTSIVNPERTVDDKGKIKVEAEDNAQILMEHDKGVISHVMCGFNYF
                     DPHGHEAGNQSLHSIQIYGDYGNMRLIGYDWETNGVMLDVHWDKPAELVCTDKGGYEW
                     QEGARVVGESLVNGTEPRINVEHALHVLEIIEAARKSSATGMKVKLKSKFPWPIVK"
     gene            complement(1218..3713)
                     /locus_tag="PLU11_13015"
     CDS             complement(1218..3713)
                     /locus_tag="PLU11_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPN60098.1"
                     /translation="MLRQGFYSIYILAGIFLFIQPLTAQLRTVDAVTSYRKIPGGIEG
                     KTRFTRFEIKAWSANTIRVRITKEKTFNPVQYALAADTGAFYPAVVNSMEKQIEIVTP
                     TLTCVVEQSPYFRVIFKNAAGEIIQEDMPGAGFGTSWLGDKISLYKKMQEGERFLGLG
                     EVLGPLDKRGMAFTLNNTDTYKYGDPRLSMYISIPFYIGVHHRQVYGLYFNNTWKTQF
                     NFGISTPQFSSINADGGEADYFFFYDQSISGILNQYSTITGKMPLPPKWSLGYHQSRC
                     SYYPQSNVELLGETFRRKQIPVDGIVLDADYQQDYKPFLVNKERFPDLPGLSRKLESM
                     HISLTASVYPGVKLDSTYDSYTDGLKNDVFIKYADGRLFETEIAPLRCYLPDYTNPKT
                     RAWWIGKMKWMEENGIRGYWNDMNEPAVGGSYLPDNLLFDFDGRKAYAPEAKNVYGFQ
                     MARSSYDAAVRHGKGKRPFVLTRSAFAGVQRYAAVWSGDNMATNEGLLSGVLLNSQMG
                     LSGMPFVGPDLGGYIGDGSKDLFKRWMQVGVFSPYLRNHKEFFATANEPWSYGEEAEA
                     ISKTYIGFRYRLMPYLYSKFYEAAQTGLPVARSLALDYPFDKNIYDPVYQYQFLFGDA
                     IMVVPVTTEEKSKKLYLPEGEWYDLFTDLLYSGNREINCSTPAYSIPLFVKSSAIIPV
                     QGLVQSTRDIASDTLQIHLYKGTTNNRFLFYEDAGEGNGYKEGEFAKRFIVYDPSARQ
                     LKLTVTEGIYTSDYKKIQWVFHGFGMEIVRVNVNGIVQEPLHYTGRLLDPLKDLEDYY
                     GKDRVKGLYLADPVLKQVSLVTANPRSELIIQW"
BASE COUNT          989 a          993 c          797 g         1049 t
ORIGIN      
        1 gatccgagaa tagcgagttt cataagctta tcttatttaa caatgggcca gggaaattta
       61 cttttcaatt ttactttcat gccggtggcg gaggatttac gggccgcttc aatgatctcc
      121 agcacatgta aggcatgttc cacattgatc cgtggctctg tgccattgac cagactttcg
      181 ccgactacgc gggcaccttc ctgccactca tagccacctt tatcggtaca gaccagttcg
      241 gccggcttat cccaatgtac atccagcatc actccattcg tttcccaatc atacccgatc
      301 aaccgcatat ttccataatc accatagatc tgaatggaat gtaaggactg attgccggct
      361 tcatgtccgt gcggatcaaa ataattgaaa ccacacatca catgactgat cacaccttta
      421 tcatgctcca tcaatatttg tgcattgtct tccgcctcca cttttatttt tcctttatca
      481 tccaccgtac gctccggatt aacgatactg gtcatggcca tgatgctgcg ggcgggtccc
      541 aataaaccgg ttaaggtggc gatattatat accccgagat ccggcatact gcccccgttc
      601 ttttcataga agaaagcgga ccaggtagga cccgtatgtc cgtattgacc atgagccgcg
      661 gccacccgtc ccagttttcc ttcctggatc attttactca tataggcaaa ctgcggactg
      721 ttaactactg cgggagcgcc ccagattctt aatttttttg ttttagccag atccagcaag
      781 gccttgccct cggcataggt attggccata ggtttttcac tccacacatg cttgccggcc
      841 ataagggctt tcttattcaa ttcaccatgc acctgcatat cggtcagagt gaccatcata
      901 tcaaagggca cccctttgag catttcatcg atatggccgt aggtagcggc atttacttta
      961 tactctttgt tctgcgccac tgctcgttcg ggctttatat cacaaagact caccacttca
     1021 atccacttcg atgaaagcaa ctggggtata taacggttgc ttacactgcc gcaaccgatt
     1081 accgccaccc gcagtttttt atccggcaga gaagccgccc agccttccag tgaggaaacg
     1141 agtaatgccg ccccggccag agccgtggtc ttcaaaaatt tttcgcggga aattgtatca
     1201 gacatggtta tttattttta ccattgaata atcagttcgc tgcggggatt agcagtcacc
     1261 aggctgacct gctttaatac cggatcagcc aggtacaagc ctttcaccct gtcttttcca
     1321 taataatctt ccaggtcttt caagggatcc agcagtcgtc cggtatagtg caatggctcc
     1381 tgaacaattc cattgacatt tacccgaact atttccattc caaatccatg gaagacccat
     1441 tggatttttt tatagtcaga ggtataaatt ccttctgtaa ccgtgagttt cagttgacgg
     1501 gccgaaggat catacacgat gaaacgtttg gcaaattccc cttctttata cccattgcct
     1561 tccccggcat cttcataaaa aagaaagcgg ttattagtag tacccttgta caaatggatc
     1621 tgtaaagtgt cggaagctat atcgcgggtg gattgtacca atccctgcac cggaatgatc
     1681 gcggaagact ttacaaacaa cggaatggaa tacgccggtg tgctgcaatt aatttcccgg
     1741 tttccggaat acaacagatc cgtaaaaagg tcataccatt ccccttcagg cagatacagt
     1801 tttttgcttt tctcctccgt tgtcacaggc acaaccatga tcgcatcccc gaagagaaac
     1861 tggtattggt aaacagggtc atatatgttt ttatcaaacg gataatccag ggcgaggctg
     1921 cgggccaccg gcaaaccagt ttgtgccgct tcataaaatt tggaatagag ataaggcatc
     1981 agccggtagc ggaatccaat ataggttttg gaaatggctt ctgcttcttc gccataactc
     2041 cagggttcat tggccgtcgc aaaaaattct ttatggttgc gcagataagg agagaacacc
     2101 cccacctgca tccagcgttt gaagagatct ttgctgccat ctccaatata tccacccaga
     2161 tccggcccta caaagggcat tccggataaa cccatctggc tgttcaataa aacaccactg
     2221 agcaatcctt cattggtagc catattatct ccgctccata ccgccgcata tcgttgtaca
     2281 ccggcaaaag cggagcgggt gagtacaaaa ggacgtttcc cctttccatg tctcacagcg
     2341 gcatcataac tacttctggc catttgaaat ccatagacat tctttgcttc cggagcatag
     2401 gctttacggc cgtcaaaatc aaagagcagg ttatccggca ggtaactgcc tcctaccgcc
     2461 ggttcattca tatcattcca gtaaccacgg atcccattct cttccatcca tttcattttg
     2521 ccgatccacc atgctcttgt tttaggattg gtataatccg gcaaatagca gcgcagcgga
     2581 gcaatctcgg tttcaaacag acggccatcc gcatatttaa taaacacatc attttttaac
     2641 ccatcggtat aagaatcata ggtgctgtcc agtttcaccc cgggatatac cgaagccgtg
     2701 agggaaatat gcatgctttc cagtttcctg gataaacccg gtaaatccgg aaaacgttct
     2761 ttgtttacca ggaatggttt atagtcctgc tggtaatccg catccagcac aatcccatcc
     2821 accggtatct gtttgcgccg gaaggtttca cctaataact ccacattgga ctgcgggtag
     2881 taactgcaac gggactgatg atacccgaga ctccatttgg gaggcagggg catcttaccg
     2941 gtaatcgtac tgtattgatt caggataccc gaaatacttt gatcataaaa gaaaaaataa
     3001 tcggcttccc ctccatcggc attgatcgat gaaaactgcg gcgtggaaat accaaagttg
     3061 aattgcgtct tccaggtatt attaaaatac aacccataaa cctgccggtg atgcaccccg
     3121 atataaaacg gaatactgat atacatggat aaacgcgggt ctccgtactt ataggtatcc
     3181 gtattgttga gggtgaaagc cattcctctt ttatccaacg ggccaagcac ttcgcccagt
     3241 ccgagaaaac gctcgccctc ctgcattttt ttatacagac ttatcttatc gcccaaccag
     3301 ctggtaccaa atcctgcacc cggcatatcc tcctggatta tttcaccagc tgcattctta
     3361 aaaatcacgc ggaaataagg cgattgctcc accacacagg tcagggtggg tgtaacgatt
     3421 tcaatttgct tttccatgct gtttaccaca gccggataga aagcgcccgt gtctgccgcc
     3481 agggcatact gtaccgggtt aaatgttttt tctttggtga tccgcacccg gatagtattg
     3541 gccgaccagg ctttgatttc aaaacgggtg aaccgggttt taccttcaat ccctcccggt
     3601 attttccggt aactggtaac tgcatccacc gtacgcagct gggcagtcag cggctggatg
     3661 aacaggaaaa ttcccgccag tatatatatg ctataaaatc cttgccttaa catgcgtttg
     3721 attagtccgt ttaaaattca agagatgcca tcaccggaaa atgatcggaa ggatacttca
     3781 tgtcataaga atccgtaatg gtggcaaatt ttctgaccgt aagttttt
//
LOCUS       DAOPMW010000335         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28491_length_3819_cov_7.844315, whole genome shotgun sequence.
ACCESSION   DAOPMW010000335 DAOPMW010000000
VERSION     DAOPMW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28491_length_3819_cov_7.844315"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1818)
                     /locus_tag="PLU11_13020"
     CDS             complement(121..1818)
                     /locus_tag="PLU11_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60099.1"
                     /translation="MSVKLKTKVALGGIFLFALLMLVGAVSFFYLNRITDEARGIIKD
                     NYETLNYSRDMLKDMDELNGPDSLSAILRFEKNLRLQEDNITEPGEGERTVSLRQHFN
                     LLKQVESTDSIQSLMRKDISSIMELNLRALNNKNLAAQASADNAKTIITVILTICLLL
                     GVTFIVNFPSLVADPVARLTEGIKGVGARNYSERIHLNRKDEFGELANAFNTMAERLD
                     EYEHSNLAKILFEKQRAEAVINSLKDASIGIDNKGMVLFANQEALQLLSLSNADILGR
                     SQEEVKKQNDLFRFLVNEQNNIPFKIVVEGKENYFTKEIMELTHQDQKTGVVIVLKNI
                     TPFKELDVAKTNFIATVSHELKTPLASSDFSLKLLEDERIGTLTHEQKELVQNLKEDN
                     KRLLRILSELLDMSQVESGKIQLTLQDTSATLLVEKAIAAVSAVAREKKIVVHMKKDE
                     VGGEVYADAEKCAWVLNNFLTNAIRYSPEGQVIDVSIRPSGNQLEFSVRDFGKGIDPQ
                     YKEKIFDRYFRVPGSKEGTGLGLAICKEFIEAQGGKIGVESTPGEGSLFYFTLPLKE"
     gene            complement(1818..2939)
                     /locus_tag="PLU11_13025"
     CDS             complement(1818..2939)
                     /locus_tag="PLU11_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HPN60100.1"
                     /translation="MPDKEANIWLEKLKKERKGKLKIYIGMSAGVGKSYRMLQEAHTL
                     LRNGVYVKIGYIETHGRKETEALVEGLSEVPRRKVFYKGKELDELDVQAILLLHPEVV
                     IVDELAHTNIPGSKNEKRWQDVLDIIEAGINVITAVNIQHIESINDDVKAITGVEVKE
                     RVPDKILQLADEVVNIDLTADELITRLKEGKIYDHSKVQQALANFFQAEKILQLRELA
                     LKEVAGQVERKVDHELTNKDRSWRHEHIMACISSNHEMAQNIIRKTARLASYYNSKWV
                     VLYVQTAKESVDNIPLAAQRHLINNFKNATELGAEVIRIKAGNVAQGILKMVEERHIT
                     TLCIGKPHLNLYQIILRTNVFNQLLKTLSSKDVDLIILS"
     gene            complement(2941..>3819)
                     /locus_tag="PLU11_13030"
     CDS             complement(2941..>3819)
                     /locus_tag="PLU11_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPN60101.1"
                     /translation="GFIKANYTADRVRANLALAAGTYMNANYAAEPGVLKNVYEANTG
                     VKLSKKKNFWLDAGIFASHIGFESAISKDCWTPTRSLLADNSPYYEAGAKLTYTTADG
                     KWLLSGLALNGWQRIKRVDGNSRMSWGTQVQYKPSDKVLLNYSTFIGTDKPDSARLNR
                     LFHNFYGIFSLADQWGIILAVDAGTEEKTPSGNKLNTWYSPVVILQYAVSDKWKMAGR
                     YEYYSDKNGVIIASNSPNSFQTNGYSFNVDHLPAGNVSLRLEARCLAAKEALFSKGTG
                     FSKSAFFITSSIAVSF"
BASE COUNT          945 a          966 c          759 g         1149 t
ORIGIN      
        1 taacagctaa caacgaacac ctctcagaat gggctgtttc ctaacaacca acagctaaca
       61 acgaacacct ctcagaatgg gctgtttcct aacaactaac agctaacaac gaacagcttc
      121 ttattctttc agtggcaacg tgaaataaaa caagctccct tcaccgggag tagattcgac
      181 tcctattttt ccaccttgag cttcgataaa ttctttgcag atcgcgagtc caagtccggt
      241 tccttctttt gaaccgggga cccggaaata ccggtcgaat attttttcct tgtattgggg
      301 atcaatgcct tttccaaaat cgcggacact gaattcgagc tggttaccgg atggccggat
      361 gcttacatca atcacttgcc cctcggggga ataacgaatc gcattggtca gaaaattatt
      421 cagcacccag gcacactttt cagcatcggc atagacctcg ccccctacct catccttctt
      481 catgtgaacc actatttttt tctccctggc tacggcagag accgctgcaa ttgctttttc
      541 caccagcaaa gtggcggatg tatcctgcag cgtcagttga atttttccgc tttctacctg
      601 ggacatatcc aggagctcac tcagtatgcg taataaccgt ttattatctt ccttcaggtt
      661 ctgtaccagt tccttctgtt catgggtcaa tgtaccaata cgttcatcct ccaggagttt
      721 caggctgaaa tcagaagaag ccaggggtgt tttcagttca tgggagaccg tagcgataaa
      781 attcgtttta gctacatcca gttctttaaa aggggtaatg tttttcagca cgatcaccac
      841 cccggttttc tgatcctggt gcgtcagttc catgatttcc ttggtaaaat aattttcttt
      901 tccctccact actattttaa acggaatatt attttgttca ttgaccagga accggaaaag
      961 atcattttgt tttttcactt cctcctggga acggcccagg atatcggcat tggataaact
     1021 gagtaactga agggcttcct ggttggcaaa taacaccatt cccttattat caatgccgat
     1081 actggcgtct ttgagtgaat tgataactgc ctccgcccgt tgcttttcaa agagaatttt
     1141 agccaggtta ctatgttcat attcatccag tctttccgcc atggtattaa acgcattggc
     1201 cagttcgcca aattcatctt tgcggttcag gtgtatacgt tcgctgtaat tcctggcccc
     1261 aactcccttt atcccttccg tcaaccgggc caccggatca gccaccagtg aaggaaaatt
     1321 gacaataaag gtcaccccca gtaacaggca gatcgtaaga atcacggtaa tgatcgtctt
     1381 tgcattgtct gccgaagcct gggcggccag gtttttatta ttgagtgccc gcaaattcag
     1441 ttccatgata ctgctgatat ctttccgcat cagcgactga atggaatctg tagattccac
     1501 ctgttttaat aaattgaaat gctgccggag agaaaccgtc cgttcacctt caccgggttc
     1561 ggtgatatta tcttcctgca accgaagatt tttttcaaaa cgaagaatcg cggacaagct
     1621 atccggcccg ttcagttcat ccatatcttt caacatatcc ctgctgtaat tcagggtttc
     1681 ataattgtct ttaataatac ccctggcttc gtccgtaatc cggttcaggt agaaaaagct
     1741 gactgctcct accagcatca ggagggcaaa aaggaatata cctcccaggg ctacttttgt
     1801 tttaagtttg actgacatca ggataatatt atcaggtcta catccttact ggagagtgtt
     1861 ttgagcaact ggttaaaaac attggtccga aggataatct ggtacaaatt taaatgaggt
     1921 tttccgatac acagggtggt aatatgccgt tcctctacca ttttaaggat gccctgggcc
     1981 acattcccgg cttttatacg gatgacttcc gccccaagtt cggtggcatt tttaaaatta
     2041 ttgatcaggt gacgctgagc ggccagcggg atattatcca ccgactcttt agcagtttga
     2101 acatacagca ccacccattt gctgttataa taggaagcca gccgcgccgt tttccggatg
     2161 atattctgtg ccatttcatg attggaggat atacaggcca taatatgctc atgccgccag
     2221 gagcgatctt tgtttgtcag ttcatgatct actttccgtt ccacctgtcc ggccacttct
     2281 ttcaatgcca gttcccgtaa ttgcaggatc ttctccgcct ggaaaaagtt tgccagcgcc
     2341 tgttgcacct tactgtgatc atagattttc ccttctttta accgggtaat caattcgtcg
     2401 gctgtaagat caatattcac tacctcatcg gccagttgca gtattttatc cggcaccctt
     2461 tctttcacct ccaccccggt aatggctttt acatcatcat tgatactttc aatatgctga
     2521 atattaacag ccgtaatcac atttataccg gcttcaataa tatccagcac atcctgccag
     2581 cgtttttcgt ttttacttcc gggaatattg gtatgcgcca gttcgtccac gatcaccact
     2641 tccgggtgga gcaacaggat cgcctgtaca tccagttcat ccagttcctt gcctttataa
     2701 aaaacctttc gtctgggcac ttcggataat ccttccacga gcgcttcggt ttctttccgt
     2761 ccatgggttt cgatataccc gatttttaca taaaccccgt tccggagtaa ggtatgggct
     2821 tcctggagca tccgatagct tttacctact ccggcactca taccaatgta gatcttgagc
     2881 tttcctttac gctctttctt taatttttca agccatatgt ttgcttcttt atcaggcata
     2941 tcaaaaagaa actgcaatag atgaggtaat aaaaaaagct gacttgctga aaccggttcc
     3001 tttgctgaaa agagcttcct ttgccgccag gcacctggct tccagtcgca gactgacatt
     3061 cccggccggc agatgatcca cattgaaaga ataaccgttc gtctgaaagc tgttgggact
     3121 gttactggca atgatcaccc cgtttttgtc actgtaatat tcatagcggc cagccatctt
     3181 ccatttatcc gaaacggcat attgcaaaat cactaccggg ctataccagg tgttgagttt
     3241 attacctgag ggtgttttct cttctgttcc ggcatccacc gccaggataa ttccccattg
     3301 atcagcaagt gaaaaaatgc cgtaaaagtt atgaaacaac cggttcaacc ttgcgctgtc
     3361 gggtttatct gttccaataa aagtgctgta attcaacaac actttatcag aaggtttgta
     3421 ctgtacctgt gtaccccagc tcatgcgtga attaccatcc actcttttga tccgttgcca
     3481 gccattcagg gccaatccac tcagtaacca tttaccatcc gcagtggtat aggtgagttt
     3541 tgcccccgct tcataatagg gagaattgtc agccaggagg ctccgggtcg gtgtccagca
     3601 atctttactg atggcacttt cgaaaccgat atgggaagca aaaatacccg catccagcca
     3661 gaaattcttt ttcttactca gcttcactcc tgtattggct tcatacacat tttttaatac
     3721 acccggctct gcggcatagt ttgcattcat gtaagtacct gcggctaacg cgagattggc
     3781 tcttactcta tcagcggtgt aattggcttt tataaaacc
//
LOCUS       DAOPMW010000336         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28608_length_3807_cov_5.402719, whole genome shotgun sequence.
ACCESSION   DAOPMW010000336 DAOPMW010000000
VERSION     DAOPMW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28608_length_3807_cov_5.402719"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1990)
                     /locus_tag="PLU11_13035"
     CDS             complement(<1..1990)
                     /locus_tag="PLU11_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HPN60102.1"
                     /translation="MYKLLLFPVILLTSLISTANTILVRNSEELQEAEKKAMPGDIIL
                     LKNGTWNNVLLRLNAKGTESAPVSFRAETAGKVIITGQSRLEIGGSYIVVEGFLFTNG
                     FAGKQPVITFRSGKDQLASHCRVTNTVIDGFNNPGRMEENNWITFYGKNNRLDHCSFQ
                     NKLNLGVLLAVILEDERSRENHHSIDHNYFGYRIPLASNGGEMIRVGVSQHCEFNSNT
                     KITANFFEKCDGETEIVSIKSGSNEVTNNIFRECQGSVVLRHGNYNIVSGNYFLGNNK
                     PGSGGVRVINKGQQVLNNVFYQCRGTDFRSPLAVMNGIPNSPAHRYVQVTDAVISGNT
                     FFECTPATFCEGSDAERTLPPDQVTVSNNNFYNTRDSIIYKSYDRMDGILFSNNLVNL
                     KARQQLPGGFTKGNDAPAKKAISMISQVEKKTRLGAGAGWYNKNNSQPSISWKQVNCP
                     DLLSLTKALASPEPVAIRLTGKEYKTDQPLQITKRVRISSTGTGYTEFSSSPMESLFI
                     IEGKGQLQLDKLRLDASGLQVSSFISSSKNAPSNHFNLQLSNSQIKNLGKQFPAMSLI
                     FANKSMVADSIIIRHCTFTDFNAGLLHMTEEKDNKGYYNAEKIVISNNSITNLQGALM
                     AIYRGGNDESTMGPDLTFSNNRIENCNSESTTPLISLYG"
     gene            complement(1998..3035)
                     /locus_tag="PLU11_13040"
     CDS             complement(1998..3035)
                     /locus_tag="PLU11_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN60103.1"
                     /translation="MNKPATIKDIAKQLNISISTVSRAIRNAADVNPETKRAVMALAE
                     ELDYQPNRLALSLKQKQTHNIGVLVPNLDYVLSTMVKGIDEVALEAGYTVMVCQSNES
                     FGREIVNTRRLLDSLVDGFIISVSSETKVFDHFKKILDKKMPVVVFDRVTPFLQAPGV
                     RIDNEDGGFQATEHLIEQGYKRIAILAGPKNLQISNQRMEGYLKSLKKHKIKPDKELI
                     IYCDFNQDYAYLATKELLAMKKRPDAIFTISDRMAIGAMLAIKEKGLKMPHDIGLVGF
                     NNEPVTSLVTPGISSVDQPAFELGKIAAKLFIEKMHRDTDMSQVEEVLKPKLIIRESS
                     LRILPRGGRLK"
     gene            3359..>3807
                     /locus_tag="PLU11_13045"
     CDS             3359..>3807
                     /locus_tag="PLU11_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN60104.1"
                     /translation="MNHFSGKVAIVTGGSRDIGRQVCLKLAAGGARVCVNYFGNRDLA
                     DETIKQIKAVGGEAIVAYGDMTKPADVKQVVDACVAAFGNNVHILVNVAGGLMGRKVL
                     ADLDEAFWDAVIDVNLKTAYLVTRAVVPYMNEGGAIVNFSSQAARDGG"
BASE COUNT          919 a          831 c          898 g         1159 t
ORIGIN      
        1 cgccatacag gctgatcagc ggtgttgttg attcgctatt gcagttttct atccggttat
       61 tgctgaaggt tagatccggc cccatggtac tttcgtcatt acctccacga tagatggcca
      121 ttaatgcacc ttgaaggtta gtgatgctgt tgttgctgat cacgattttc tccgcgttat
      181 aatatccttt attgtctttt tcctcggtca tgtgcagcaa cccggcatta aaatccgtaa
      241 aagtgcaatg gcggataatg atactgtcgg ccaccatgct tttattggca aaaatgaggc
      301 tcatggccgg gaattgcttt cccaggtttt taatctggct attcgagagc tgcagattga
      361 aatgattgga cggagcattt ttgctgctgg aaatgaatga tgaaacctgc aggcccgatg
      421 catccagcct gagtttatct aactgcaatt gcccttttcc ctctatgata aacaggcttt
      481 ccatcgggga ggagctgaat tcggtataac cggtgcctgt gctgctgatc cggacccgtt
      541 tggtaatctg taagggttga tctgttttgt actctttgcc ggtcagccgg atggctaccg
      601 gttcaggaga agccagtgcc ttggttaagc tcagcaggtc cgggcaattc acctgtttcc
      661 agctgatgga gggttggctg ttatttttat tataccagcc ggctcctgcg cccagccggg
      721 ttttcttttc cacctggctg atcatgctga ttgctttttt tgcgggcgca tcatttccct
      781 ttgtaaatcc gcctggcaat tgctgacggg ccttaagatt gaccaggttg ttgctgaata
      841 agatgccatc catccggtca taacttttat aaatgatact gtcccgggta ttgtagaagt
      901 tattattgct tacagttacc tgatcaggtg gtaatgtccg ttctgcatca ctgccttcac
      961 agaaagtggc cggggtacat tcgaaaaaag tattcccact gataacagca tccgttacct
     1021 gtacataccg gtgtgcaggg gagttgggga tgccgttcat tacggccagt ggtgaacgga
     1081 aatcggtgcc ccggcattgg taaaatacgt tgttgagcac ctgttgccct ttgtttatca
     1141 cacggacgcc cccgctgccg ggtttattat tgcccaggaa ataattgccg gatacaatat
     1201 tgtaattgcc atgacgtaac acgaccgagc cctggcattc gcggaatata ttattggtaa
     1261 cttcattact accggatttg atagagacga tttctgtttc cccatcgcat ttttcaaaaa
     1321 aatttgccgt gatttttgta ttcgaattga attcacaatg ctgggataca cccactctga
     1381 tcatttctcc gccattggat gccaggggta tacggtaccc aaaatagtta tgatcaatgg
     1441 agtgatggtt ttcccggctt cgttcatcct ccaggatcac agccagcagc acaccgaggt
     1501 tgagtttgtt ttgaaagctg caatgatcca gccggttgtt tttgccataa aaagtgatcc
     1561 agttattctc ctccattcgt ccggggttat taaagccgtc tatcactgta ttggtgacac
     1621 gacagtggct ggctaactga tcctttccgc tgcggaatgt aatgaccggt tgcttacctg
     1681 caaatccgtt tgtgaataaa aagccttcaa ccacgatata actgcctccg atttcaagcc
     1741 gggactgacc ggtgatgatt actttacctg ccgtttctgc ccggaagctg accggtgctg
     1801 attcagtacc ctttgcgttc aggcgcagca ggacattatt ccaggttccg tttttcagca
     1861 ggataatatc acccggcatg gctttttttt ctgcttcctg tagttcttcg ctgtttctta
     1921 caagaatcgt attggctgtg gaaataaggg aggtcagtaa aatgaccgga aataaaagaa
     1981 gtttgtacat ggtgctttta ttttaaacga ccgccacgag gcaggatacg aagtgatgat
     2041 tcccggatga tcagtttggg ctttaatact tcttcaacct ggctcatatc cgtatcgcgg
     2101 tgcatctttt ctataaatag tttcgcggct atttttccca attcaaatgc gggttggtct
     2161 acactggata ttcccggggt cacaaggctg gttaccggtt cgttattaaa tcccactaaa
     2221 ccgatatcat gcggcatttt taaccctttt tcttttatcg ccagcatggc acctatggcc
     2281 atccggtcac tgatggtgaa aatggcatcg ggtctttttt tcatagccag gagttccttg
     2341 gtggcgaggt aggcataatc ctggttgaaa tcacagtaga tgatcagttc tttatcgggc
     2401 ttgatcttat gtttttttaa gctcttcagg tatccttcca tccgctggtt actgatctgc
     2461 aggttcttcg gtccggccag aatagcgatc cgtttatagc cctgttcaat taaatgttcg
     2521 gtggcctgga aaccgccatc ttcattatca atccggacac cgggtgcctg caggaaagga
     2581 gtaacccggt caaataccac cacgggcatt tttttatcca gtattttttt gaagtgatca
     2641 aagactttgg tttcactgga aacggaaata atgaatccat caaccagact atccagcaaa
     2701 cgtcgggtgt ttacaatttc acggccaaag gattcattac tctgacaaac catcacggta
     2761 tacccggctt ccagggccac ttcatcaatt ccttttacca tggtggaaag cacataatcc
     2821 aggttgggta ccaatacccc gatattatgg gtttgctttt gtttaagact tagtgcaagc
     2881 cggttaggtt gatagtccag ttcttctgcg agggccatta ccgctctttt tgtttccggg
     2941 tttacatccg ctgcgttacg gatggccctg gataccgtgg aaatggaaat attcagctgt
     3001 ttggctatgt ctttaatggt ggcaggctta ttcatggttt gcaggcatca gggaataaag
     3061 ctatgaagaa gaagtttatg aaaatcccgg cagcgttgcc ggtaacgtta tcggtctctt
     3121 ttatttagaa aatatataaa aaatataata aaaatcttaa tttgtttaaa aaatgtacat
     3181 ttaattaata atattctaat cgacaaaaaa aagcccacct gattattcca cttaatatta
     3241 ccatcgaaag cgtccggcat gtcgaaacaa tcagcagaaa tcgggtattg cttcatttgc
     3301 cgctccggtt ttttaatcat acccttctct attcatttta gcgtctaaaa ttttttttat
     3361 gaatcatttt tcaggtaaag tggctatcgt tacaggtggt tccagggata tcggtcgaca
     3421 ggtttgttta aaacttgcag ccggcggcgc aagggtatgt gtgaattatt ttggaaacag
     3481 agatctggcc gatgaaacga tcaaacaaat taaagcggtc ggcggtgaag ccatcgtggc
     3541 ttatggggat atgacaaaac cagctgatgt taaacaggtg gtggatgcct gtgtggcggc
     3601 attcggaaac aatgttcata tactggtgaa tgtggcaggc ggactgatgg gtcgtaaagt
     3661 gctggctgat cttgatgaag cattctggga tgcggtgatt gatgtaaacc tgaaaacagc
     3721 ctacctggtg accagggcag tggtacctta tatgaacgaa ggaggggcta ttgtcaactt
     3781 ctcctcacaa gcggcccggg atggagg
//
LOCUS       DAOPMW010000337         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28689_length_3799_cov_7.984241, whole genome shotgun sequence.
ACCESSION   DAOPMW010000337 DAOPMW010000000
VERSION     DAOPMW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28689_length_3799_cov_7.984241"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLU11_13050"
     CDS             <1..222
                     /locus_tag="PLU11_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60105.1"
                     /translation="YSNPDGITYSEETCRRLASMRTAAPDFRIFWDNAYFIHALDPEH
                     PDRVPEMLSLCAVTCVLTVPSVITLPPIS"
     gene            complement(219..1085)
                     /locus_tag="PLU11_13055"
     CDS             complement(219..1085)
                     /locus_tag="PLU11_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPN60106.1"
                     /translation="MEKVQWKVEGMDCTTCALNIHKYLDKKGAANIKVNFATGDVSFD
                     RSADLAEEKLATGVTDLGYKVITASASGHAHGHDHDEIDTPFFTSHLQRFWFCLPFTA
                     LLMLHMVPGLHIHWLMNHWVQLGLTIPVYIIGMSFFGKSAWKSLRNGMPNMNVLIALG
                     STAAFVYSLYGSLTGQAEQYMFYETAATIITLVFFGNYMEDASIASTQRELNKLAKSQ
                     RVMANMIAFDDEHKEQIFPVENTALRVGDLLLIKSGEQVPIDCKILWGNVHVNESIIT
                     GESMPVNKKAKD"
     gene            1177..1602
                     /gene="tsaE"
                     /locus_tag="PLU11_13060"
     CDS             1177..1602
                     /gene="tsaE"
                     /locus_tag="PLU11_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPN60107.1"
                     /translation="MEWKFSVETIKMTAASFLNQAGSASVFAFHGPMGAGKTTFIHAL
                     CEELGVKDVVGSPTFSIINEYRYPADGKMKPLYHMDLYRLRDEEEAQRAGVEDALYSG
                     HICLVEWPEKAPGIFPEHTINVFIELVDNQTRRLRIANN"
     gene            1645..2859
                     /locus_tag="PLU11_13065"
     CDS             1645..2859
                     /locus_tag="PLU11_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPN60108.1"
                     /translation="MSQQKPKISTTFSYETLEETLDIRPKAEGMMIGIPKEIAFQENR
                     VALTPDAVGVLVSNGHQVIIEHNAGEASHFRDKDYSEAGAKIVYDRESVYKAPILVKS
                     APVIEEDLPLLQMNQIIISPIHLSVLKAMILEKMMEKKITAISFENLKDDSDSYPIVR
                     SMSEIAGSAVMLIAAQYLGSANHGKGVLLGGISGIAPTKVIIIGAGIVGEYAARAALA
                     LGASVKVFDNSVYRLKRLQNNIGQRMWTSVLEPRLLAKQLKTCEVAVGALGSQTGRTP
                     LVVTEEMVSNMRPGSVIIDVSIDRGGCFETSEITSHEHPIFMKYGVIHYCVPNIPSGF
                     SRTASQAISNVLMPLLLEAGEEGGFENLVWHKLHLRNGIYLFKGALTNFYLSQRFDLK
                     YTDLNLLIASQR"
     gene            complement(2952..>3799)
                     /locus_tag="PLU11_13070"
     CDS             complement(2952..>3799)
                     /locus_tag="PLU11_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN60109.1"
                     /translation="FISTALTSQEAKDHISILGVTDAGRQELLKNLSITASRKLSPWL
                     KSSISLAYAERMPTATELYGFYLFNAQDGYDYLGNPGLKPEQSLQGEFSLITLGKKAR
                     LKVTAFYNQIRHFISGTVNPAFSTMTIGANGVKSFSAVPHATLAGLETSLFLKPLPQL
                     DIVSTLRYTDGNDNTGDPLPMIAPLKSISSVRYQFNPFFLQLETELAAAQNKISIKYG
                     EDRTNGYALLHIRSGFPFRLAGKETDLQFGVENLFDKHYHEHLDWGNVKRQGRNAYLM
                     LKWFF"
BASE COUNT          975 a          877 c          912 g         1035 t
ORIGIN      
        1 tacagcaacc cggacggcat cacctattcc gaagaaacct gcaggcgcct cgcttccatg
       61 cgcaccgccg cgccggactt ccgcattttc tgggacaacg cgtattttat ccacgcgctg
      121 gatccggagc atccggaccg cgtcccggag atgctttccc tttgcgcggt aacctgtgtt
      181 ttaacggtgc catctgtaat tacgctgcca ccaatcagct agtcttttgc ttttttattt
      241 accggcatac tttccccggt aatgattgat tcattcacat gcacattccc ccataatatt
      301 ttacaatcaa tcggaacttg ttctccgctt ttgatcagta acaagtcacc aacccgcagc
      361 gcggtatttt ccaccggaaa aatctgttct ttatgttcat cgtcaaaagc aatcatgtta
      421 gccatcactc tttgtgattt ggccagctta tttagctccc gttgcgtgga ggcgatagag
      481 gcatcctcca tatagttacc gaaaaaaacg agggtgatga tggtggcagc agtttcataa
      541 aacatatact gttctgcctg gccggtcagt gatccataga ggctgtaaac aaaagccgct
      601 gtggaaccca aggcgatcag tacattcata ttgggcatcc cgttccgcag gctcttccag
      661 gcgctctttc cgaaaaaact cataccaatt atataaaccg ggatggttaa gcccagttgc
      721 acccaatgat tcatcagcca gtggatatgc aggcctggga ccatatggag cattaacaag
      781 gctgtaaaag gcaggcagaa ccagaatcgt tggaggtgag aggtaaagaa tggcgtgtcg
      841 atctcatcgt gatcatggcc atgtgcatgc ccgcttgctg aggcagttat tactttgtat
      901 ccaaggtccg ttacccccgt ggcgagtttt tcttcggcta aatctgcgct gcggtcaaaa
      961 ctcacatcgc cggtggcaaa attgactttg atattggcgg cacctttttt atccaggtat
     1021 ttgtgaatgt tcagcgcaca ggtggtgcag tccatgccct ctactttcca ttgtaccttt
     1081 tccatagggt aaagttacca aaagggggaa agccggaggt tcctcaagcg ggaatatgtt
     1141 tacaacatgg ttgctataac gccttatttt tgtggtatgg aatggaaatt ttctgtggaa
     1201 accattaaaa tgacagcggc ctcttttcta aatcaggccg ggtctgctag tgtttttgcc
     1261 tttcatggtc cgatgggggc cggtaaaacc acgtttatcc atgctctctg cgaagaactg
     1321 ggggtgaaag atgtagtggg gagtcctact ttttcaatta tcaatgaata ccgctacccg
     1381 gcggatggga aaatgaagcc actctatcac atggatcttt accggttgcg ggatgaggag
     1441 gaagcccagc gggccggtgt ggaagatgcc ttatacagtg gtcatatctg cctggtggaa
     1501 tggcccgaaa aagctccggg tatttttccg gaacatacaa taaatgtgtt tattgaactg
     1561 gtggataacc agacccgaag gcttagaatc gccaataatt aattagcttt ggagtaacga
     1621 ctgtttacct tagcaattaa cttcatgagt cagcaaaaac ccaaaataag caccactttc
     1681 agctacgaaa cgctggaaga aaccctggat atccgcccta aggcggaggg aatgatgatc
     1741 ggcatcccta aagaaattgc ttttcaggaa aacagggtgg cgctgacccc cgatgcggtg
     1801 ggggtattag tgagtaacgg ccaccaggtg atcatcgaac ataatgccgg agaagcctcc
     1861 catttccgcg ataaggatta cagcgaagca ggtgcaaaaa tcgtatacga ccgtgagtcg
     1921 gtttataaag caccaatatt agttaaaagt gccccggtga ttgaagaaga cctgccgttg
     1981 ctgcaaatga accagattat tatttccccg attcatctgt ctgtattgaa ggcgatgatc
     2041 cttgaaaaaa tgatggagaa gaagataacg gctatttctt ttgaaaacct gaaggatgac
     2101 agtgattcct atcccattgt ccgtagtatg agtgaaattg cggggagtgc agtaatgctg
     2161 attgcggcgc agtaccttgg ttctgccaat catggcaagg gtgttttgct tggcggtatt
     2221 tccggtattg ctcccaccaa ggttatcatt attggtgccg gtattgtggg tgaatatgcg
     2281 gcccgtgcgg ctcttgcgct gggtgcttca gtaaaggtgt ttgacaacag cgtatatcgt
     2341 ttaaaaagat tacagaataa tattggtcag cggatgtgga cctccgtact ggagccccga
     2401 ttactggcca aacaactcaa gacctgtgaa gtggccgtag gtgccctggg ttcacaaacc
     2461 ggacgaaccc cattggtcgt tactgaagaa atggtgagta atatgcgccc gggttccgtg
     2521 atcattgatg tgagtatcga ccggggtggt tgttttgaaa cctctgaaat tacttctcac
     2581 gaacacccca tctttatgaa atacggggtg attcattatt gtgtgccgaa tattccctcc
     2641 ggtttttccc gcaccgcctc ccaggctatc agcaatgtac tgatgccctt attactggaa
     2701 gctggtgaag aaggcggatt cgaaaacctg gtctggcaca aactccatct ccgcaacggg
     2761 atctatttat ttaaaggagc gttaacgaat ttctacctga gccagcgctt cgatttaaag
     2821 tatacggacc tcaatcttct gatcgcgagt cagcgctgaa gcaggttgaa gggttgaaag
     2881 gtttacaagt tgacaggtgt catactaaga aaaccacctt gtcaacttgt aaaccattca
     2941 acttgtcaac atcaaaaaaa ccacttcaac attaaatacg cattccttcc ctgtctcttc
     3001 acattccccc aatccaggtg ttcatgataa tgtttatcaa ataaattttc cacgccgaac
     3061 tgcaggtctg tttctttgcc cgctaatcta aatggaaaac cgcttctgat atgtagtaac
     3121 gcatatccat ttgtccggtc ttcaccatac ttaatgctga tcttattttg cgccgctgcc
     3181 agttctgttt ccagttgcag gaagaaaggg ttgaactgat aacggacaga actgatgctt
     3241 ttcaacgggg caatcatggg taacggatct ccggtattgt cgttgccatc cgtataacgc
     3301 agggtggata cgatatccag ttgcggcagg ggtttcagga acaggctggt ttcaagtccg
     3361 gcaagcgttg catgaggaac agcggaaaaa cttttcaccc cgttggcccc gatcgtcatt
     3421 gtgctgaatg caggattcac cgtaccactg ataaaatgcc ggatttggtt atagaatgct
     3481 gttaccttta atctcgcttt tttgcccagt gtaatcagcg agaattcacc ttgcagggat
     3541 tgctccggtt ttaatccggg attaccgagg taatcgtagc catcctgtgc attgaataaa
     3601 tagaatccat acagttccgt ggcagtaggc atccgttcag cgtaggccaa tgaaatactg
     3661 cttttcaacc agggacttag tttccgtgaa gcggtaatgg aaaggttctt cagcaattcc
     3721 tgtcgccctg catcggttac cccgagaata ctgatatgat cttttgcttc ctggctggtt
     3781 aaagcggtac tgataaaat
//
LOCUS       DAOPMW010000338         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_28888_length_3781_cov_11.241009, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000338 DAOPMW010000000
VERSION     DAOPMW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28888_length_3781_cov_11.241009"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..3365
                     /gene="secA"
                     /locus_tag="PLU11_13075"
     CDS             69..3365
                     /gene="secA"
                     /locus_tag="PLU11_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPN60110.1"
                     /translation="MLGFIQKIFGGSKSEKDVKKIEPYVARINQFFTSYQSLSNDQLR
                     GKTQEFRGRIKEHLSAIDTEIAQKNAAAEALPFNDLLGKDAVYQEVDKLKKDRDKKIE
                     EILEEILPEAFATVKETARRFKENSELVTTATDMDRDLSVKKDYVTIDGDKSIFKNTW
                     TAAGGQVTWNMVHYDVQLIGGIVLHQGKIAEMATGEGKTLVSTLPSYLNALPGEGVHI
                     VTVNDYLARRDQEWNGTIFEWLGITVDCIDKHQPNSEERRKAYLADITYGTNNEFGFD
                     YLRDNMVHTPEEMVQRKHHYAMVDEVDSVLIDDARTPLIISGPVPRGEDQQYHIHKPR
                     IQQLVQEQERIVRNALNEAKKRFAEGDDDPKSGGLHLFRAFRGLPKYSPVIKFLSEPG
                     VKVKMQKAENYYLQDQMRNMHIVDEELLFHIDEKNKSVELTDRGLAMITRTGEDPEFF
                     VLPDIGVKLAEVEKNVLNAEEKLQVKESILNEYAQKADRIHTVQQLLKAYTLFQIDVE
                     YIIDNGAIKIVDEQTGRVLDGRRYSDGLHQAIEAKENVKIEAATQTYATITLQNYFRM
                     YHKLGGMTGTAETEAAELWSIYKLDVVSIPTNIKMIRDDKQDLVFKTKREKYKSVIDE
                     IEVLRNAGRPCLVGTTSVEVSELLSRMLQQKKIPHNVLNAKQHAREAQVVAEAGLPGA
                     VTIATNMAGRGTDIKLGTGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQF
                     YVSLEDDLMRMFGSERIASLMDKLGYKEGDVIQHSMISNSIQRAQKKVEENNFGIRKR
                     LLEYDDVMNMQRNAVYKRRNHALFGERLALDIDTSFSAIAEGLVSSFREQEDFEGFRM
                     SCIVNFGLDTRIDEEEFKKGDINKLIDQLYTEATETYNQHKEEIQKNAIPVFRNIRHT
                     QGSHIENVVVPFTDGRKSLNVLTNLDKTVNTQGAELTNSLEKSITLAVIDDSWKEHLR
                     AMDDLKQSVQTAYLEQKDPLVIYKMEAYNQFRNMNADVNKDIVSFLSHAALPVQEENA
                     PLKEGRQEKTDMSRMKANKEEVDAAGEDYAANEKDKPVPVTVGPKIGRNDPCPCGSGK
                     KYKQCHGREA"
BASE COUNT         1161 a          869 c          920 g          831 t
ORIGIN      
        1 ttttttttgt agcaccgttt ttgacaaccc aaaacactaa caatcaataa tattttaata
       61 ttacaaccat gcttggtttc atccagaaaa tttttggcgg cagcaagtct gaaaaagacg
      121 taaaaaagat tgaaccttat gttgccagaa tcaaccagtt ttttacttcc tatcaatccc
      181 tcagcaatga ccagttgcgt ggtaaaaccc aggaattccg gggtcgtatc aaagaacacc
      241 tgtcagctat tgatacggaa attgcccaaa aaaatgccgc tgctgaagcg cttcccttta
      301 atgacctgct gggaaaagat gcggtttacc aggaagtgga caaactgaaa aaagaccggg
      361 acaaaaaaat tgaggagata ctcgaagaga ttttacccga agcttttgcc acggtaaaag
      421 aaacggcccg tcgttttaaa gaaaattcag aactcgttac cacggctacg gatatggacc
      481 gggacctgag tgtaaaaaaa gattatgtaa ccattgatgg agataaatcc attttcaaaa
      541 atacctggac cgccgccggt ggtcaggtta cctggaacat ggtacactat gatgtacagc
      601 tgatcggtgg tatcgttctg caccagggca agattgcgga gatggcaacg ggtgaaggta
      661 aaaccctggt atccaccctg ccctcctacc tcaatgcact tcccggtgaa ggggtacaca
      721 ttgtaacggt gaatgattac ctggcccgtc gtgaccagga atggaatgga actattttcg
      781 aatggctcgg cattaccgtt gattgtatcg acaaacacca gcccaacagt gaagaaagaa
      841 gaaaagctta cctggccgat atcacctatg gtaccaataa cgaatttggt tttgattatc
      901 tccgggataa tatggtgcat acaccggaag aaatggtaca gcgcaaacac cattatgcca
      961 tggtggatga ggtggactcc gtattaattg atgacgcccg tactcccctg atcatttcgg
     1021 gtccggtgcc aagaggcgaa gaccagcagt accatatcca caaaccccgt atccagcaac
     1081 tggtgcagga acaggaacgc attgtgcgaa atgcactgaa tgaagccaaa aaacgctttg
     1141 ccgaaggaga tgatgatccg aaaagcggcg gactccattt gttccgtgca ttccgtggat
     1201 tacccaagta cagcccggta atcaaattcc tgagcgaacc cggggtgaaa gtgaaaatgc
     1261 agaaagcgga aaactattac ctgcaggacc agatgcgcaa tatgcacatc gtggatgaag
     1321 aactgctgtt tcatattgat gaaaaaaaca aatccgttga actgactgac aggggactgg
     1381 ccatgatcac ccgcaccggg gaagatcctg agttttttgt attgcctgac atcggggtga
     1441 agctggcaga agtggaaaag aatgtgctga acgccgaaga aaaactgcag gtaaaggaat
     1501 ctatcctgaa tgagtatgcg caaaaagccg accgcattca tactgtgcag caattgctga
     1561 aagcctatac gctcttccag atcgatgtgg aatatatcat tgacaacgga gccatcaaga
     1621 tcgtggacga acaaaccggt cgtgtactgg atggccgccg atactcagat ggtttacacc
     1681 aggctattga ggcgaaagaa aatgtaaaga tcgaagcggc tacccagacc tatgcgacca
     1741 tcacgctgca gaactacttc cggatgtatc acaaactcgg gggtatgacc ggtacggccg
     1801 aaactgaagc cgcggaattg tggagcatat acaaactgga tgtggtgagt attcccacta
     1861 atatcaaaat gatccgcgat gacaaacagg atctcgtgtt caaaaccaaa cgcgagaaat
     1921 ataaatcggt gatcgatgag atagaagttc tccgcaatgc cggacgaccc tgtctcgtgg
     1981 gtaccacatc ggtggaagtc agtgaactgc tgagccggat gctgcaacag aaaaaaattc
     2041 ctcacaacgt ccttaacgcg aaacaacacg cgcgggaagc ccaggtggta gctgaagcgg
     2101 gtttacccgg tgcggtcacc attgccacca acatggcggg tcgtggtacg gatatcaaac
     2161 tcggtacagg ggtgaaagaa gcgggtggat tggccattat tggtacagaa cgtcacgaaa
     2221 gccgccgggt agaccgtcag ctccgtggtc gcgccggtcg ccagggagat cccggttcct
     2281 cccagttcta tgtatccctg gaagatgacc tgatgcggat gttcggcagc gaacgtatcg
     2341 cctccctgat ggataaactc ggctataaag aaggggatgt gatccagcac agcatgatca
     2401 gtaacagtat tcagcgggcc cagaaaaaag tagaggaaaa caactttggt atccgtaaac
     2461 gtctgctcga atatgatgat gtgatgaata tgcagcggaa tgcggtttac aaaagaagga
     2521 atcacgcctt gtttggtgaa cgcctggccc tggatatcga cacttccttc tccgcaatag
     2581 cagaaggtct ggttagttcc ttcagggaac aggaagactt cgaaggattc cggatgtcct
     2641 gtatcgtcaa tttcgggctc gatacacgta ttgatgaaga agaattcaag aaaggagata
     2701 tcaacaaact gatcgaccag ttatacacgg aagccacgga gacgtataac cagcataaag
     2761 aagaaattca gaagaatgcc attccggttt tcaggaatat ccgccataca caaggcagcc
     2821 atattgaaaa tgtggtggtg ccctttacag acggacgcaa atccctgaac gtactcacca
     2881 acctggataa gacggttaat actcagggcg ctgaattaac caactcactg gaaaaaagta
     2941 tcaccctggc cgtcatcgac gattcctgga aagagcacct gcgtgccatg gatgacctga
     3001 aacagagtgt gcaaacggcc tacctggaac agaaggaccc gttggtcatt tataaaatgg
     3061 aagcgtataa ccagttccgg aatatgaatg ccgatgtaaa caaggatatt gtttcattcc
     3121 tgagtcatgc cgccctgccg gtacaggaag aaaatgcccc gctgaaagaa ggccgccagg
     3181 agaaaaccga catgagccgc atgaaggcca ataaggaaga agtggatgcc gccggtgaag
     3241 attatgccgc caacgaaaaa gacaaacccg ttcccgtaac ggtaggtccc aagatcggcc
     3301 gcaatgatcc ttgtccctgc ggcagtggca aaaagtataa gcaatgccac ggaagggaag
     3361 cctaaacaag aagtagtttt tataagtaga ccggttaaaa gccggtcttt tttttgacct
     3421 tgaaaaccct caaataagta cgaagtacga actacgaagt acgaagggca ttccgtaaaa
     3481 cgaagaacat ttggttgatt aagaaaaacg ttctaaggat gggctgttcc cttctcgctt
     3541 ctagcttctt gcttctcgcc tccagggctg tttcctgaca ccagacaccc gacacccgac
     3601 acctttttcc cccatcaact tattccatta tctttaaatc aaccaaaacc agcgacttca
     3661 tgtacaggac caccaaaaag aaagtacacg gattgctcca tccggaaatt gtaggagata
     3721 aacattggga taagatcatc aatatcttca tcattgtact gatcatcctg aatgtgattg
     3781 c
//
LOCUS       DAOPMW010000339         3770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29008_length_3770_cov_6.733513, whole genome shotgun sequence.
ACCESSION   DAOPMW010000339 DAOPMW010000000
VERSION     DAOPMW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29008_length_3770_cov_6.733513"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLU11_13080"
     CDS             <1..1185
                     /locus_tag="PLU11_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPN60111.1"
                     /translation="LGNAQVKKGRLNEALSSYLEAQPLREKVGDPFFIVSDQAEISKL
                     YAKLGNTKEGIAIGEKALRIAQENNITAKLPMIYTSLASNYETANDFEKASAYYKKIS
                     DLKDSMYADANPQAIAEMRTKYETEKQDRLIEQQNNRLKMQNYLFAGIAGFVLLSSLL
                     LLSWYKRNKLRQETAMKSELMRQQETAIRAVMEAEENERQRIAKDLHDGVGQMMSAAK
                     MNLSAFESELQFDNEEQRKSFEKVINLVDESCREVRTVSHVMMPNALLKNNLGEAIRE
                     FTGKLNQKDLKVVVYTEGLEQRLDSNIETMLYRVVQECVNNTIKHAGASTLDISLIRD
                     KDGISGTIEDNGKGFDTTDAEKLKGFGIKNISSRIEYLKGSVDFDSAPGRGTVVALHI
                     PL"
     gene            1380..2069
                     /locus_tag="PLU11_13085"
     CDS             1380..2069
                     /locus_tag="PLU11_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN60112.1"
                     /translation="MEAAKTKIGLSYEPLVRMNRKISLALVDDHQIVIDGLMSLLKGH
                     EVFRLSFATTDPTEVLAKMAEHPVDILLTDVMMPGLPGNELARLVKQKFPAVKILALS
                     MSGQGDLVNEMINDADISGYVLKNVGKQELIKALGKIAAGGIYFSEEVISELQRSSER
                     KKQIEDAHLTDREIEIIRLIEKEYNNKQIAEELFISERTVETHRKNIFRKTNTNSVIG
                     LVKYAYEHKLI"
     gene            2190..3299
                     /locus_tag="PLU11_13090"
     CDS             2190..3299
                     /locus_tag="PLU11_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN60113.1"
                     /translation="MKQSLLLTCIVLTFTISQAQRSLTMLPSGGNKKASVSEQIGITA
                     VTIHYDRPGVKEREGKVWGELVHLGFADPEFGTSKSSPWRAGANENTTIEFSTDVMIE
                     GESLPAGKYGFFIAYGPNESTLIFSKNSGSWGHYYYSEKEDALRVKVKPVALDKSVEW
                     LKYEFSQQTENSAVVTLQWEKLSIPFKIEVDYLKTQIASFRQELRTDKGFYWLSWNQA
                     ANWCLQRNTNLEEALLWADSASSRNFGGEFVFAPWVTKAKILEKLGRNEEAAAVMKKA
                     LPMGSIIDIHQYGRQLLSLKQPKEALDVFKLNFTKYPNQFTTLMGLVRGYSGVGDFKN
                     ALKYANLAIPQAPDEQNKKSVQGMIDKLKEGKDVN"
     gene            complement(3413..>3770)
                     /locus_tag="PLU11_13095"
     CDS             complement(3413..>3770)
                     /locus_tag="PLU11_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN60114.1"
                     /translation="RGKYVLVDFWASWCGPCRQENPNVVENFKKFSPKNFTVLGISLD
                     RPGQKERWIEAIHEDNLTWTHVSDLQFWNNAVARLYRIQSIPQNYLLDPEGKIIAKNL
                     RGPALEAKLCELLGCN"
BASE COUNT         1136 a          845 c          892 g          897 t
ORIGIN      
        1 cttggcaatg cgcaggttaa aaagggacga ctcaatgaag cgctgtcttc ctatctggaa
       61 gcacagccct tgcgggaaaa ggttggcgat ccgtttttta ttgtatcaga ccaggccgaa
      121 atctcaaagc tttacgcaaa actgggtaat accaaagaag gtattgctat tggggaaaaa
      181 gcactccgta ttgctcagga aaataatatc acggccaagc tgcccatgat ttatacatca
      241 ctggccagta attatgaaac ggccaatgac tttgaaaaag cgtcagcgta ttataaaaaa
      301 atcagtgacc tgaaggacag catgtatgca gatgccaatc cccaggcaat tgcagaaatg
      361 agaacaaaat atgagacgga aaaacaggac cggctgattg aacagcagaa taacagattg
      421 aaaatgcaga attacctgtt tgccggtatc gccggatttg ttttgttatc cagcctgctc
      481 ttgttgtcct ggtacaaacg caataaactc cgccaggaaa ccgccatgaa gtcggaactc
      541 atgcggcagc aggaaaccgc catccgggca gtaatggaag cggaggaaaa tgaacggcaa
      601 cggattgcga aagacctgca tgacggagtg ggccagatga tgagtgcggc caaaatgaac
      661 ctgtccgctt ttgagtctga actgcaattc gacaatgaag aacaacgaaa gtcctttgaa
      721 aaagtaatta acctggtgga tgaaagttgt cgggaagtac ggactgtttc ccatgtaatg
      781 atgccgaatg cccttcttaa aaacaacctg ggtgaagcta tccgggagtt tacgggcaag
      841 ctgaaccaga aggatctgaa ggtcgttgta tatactgaag gactggaaca acggctggat
      901 tccaatattg aaaccatgct gtaccgggtg gtacaggaat gtgtcaataa cacgatcaaa
      961 catgcgggtg ccagcaccct ggatatctcc ctgatccgcg acaaagacgg gatcagcgga
     1021 actattgaag acaatggcaa aggttttgat accaccgacg cggagaaact gaaaggcttc
     1081 ggcataaaaa atatcagttc acggatcgaa tacctgaagg gttcggtcga ctttgattct
     1141 gccccgggaa gaggaacggt ggtggcgctt catattccac tttaaggtgt cgggtgtcag
     1201 ctgtcaggtg tcgggtgtca ggaaacagcc cctactaaag gcagcacctt ataaacctgt
     1261 caacctatta acctgtcaac ctcttaaacc ttttaaacct cttaaacttc tctccccagt
     1321 ttaacatttc accaactgca atcccaccct cccctgcatc tattggggaa ataataaaca
     1381 tggaagctgc caaaaccaaa attggtttat cttacgaacc attagtacgc atgaaccgca
     1441 aaatctccct ggccctggtt gacgatcacc agatcgtaat agacggactc atgagcctgc
     1501 tgaaaggtca cgaggtgttc cggctttcat ttgccacaac cgatcccact gaagtattgg
     1561 ctaaaatggc tgagcatccg gttgatatcc tgcttaccga tgtaatgatg ccgggcttgc
     1621 cgggtaatga actggcccgg ttagtcaaac agaagttccc ggctgttaaa atccttgccc
     1681 tttcgatgag tggccagggt gacctggtga atgaaatgat taatgatgca gacatttccg
     1741 gttatgtatt gaaaaatgtc ggaaaacagg agctgatcaa agcactcggg aaaattgccg
     1801 caggcggcat ctacttcagc gaagaagtaa tcagtgaact gcaacggagc agtgaacgga
     1861 aaaagcagat tgaagacgcc cacctgacag accgggaaat tgaaattatc cggctgatcg
     1921 agaaggaata caacaacaaa cagattgcgg aagaattatt catttctgag cgtacggttg
     1981 aaacccaccg gaagaatata ttccgtaaaa cgaataccaa tagtgtgatc ggtctggtga
     2041 agtatgccta tgagcataag ctgatctgat tacatttttt aaacacggga aggcttaaca
     2101 caccatttaa ccattctttc ttccattcat cacgactgtt cattacgaaa tcaggggatt
     2161 gtttagttta atggtataaa ccagcaacca tgaaacagtc cctgctcctt acatgtattg
     2221 tactgacctt tactatcagc caggctcaac gctcgttaac catgttaccc agcggtggta
     2281 ataaaaaagc gtctgtcagt gaacagattg gtataaccgc tgtaaccatc cattacgatc
     2341 gtcctggcgt taaagaacgg gaagggaaag tttggggaga gctggtacac ctgggatttg
     2401 ccgatccaga attcggcaca agcaaatcat ctccctggag ggccggtgcc aacgaaaaca
     2461 cgaccatcga attcagtacc gatgtcatga tcgaaggtga gtcactgcca gccggtaaat
     2521 acggattctt cattgcatat ggcccgaatg aatccacact gatcttttct aaaaattcgg
     2581 gttcatgggg ccattattat tacagcgaaa aagaagatgc cttgcgggta aaagtaaaac
     2641 ccgtggccct ggataagtct gttgaatggc tgaaatatga attcagccaa caaaccgaaa
     2701 acagtgcagt cgtaaccctg cagtgggaga aactgtccat cccttttaaa attgaagtgg
     2761 actaccttaa aacgcagatt gcttctttcc ggcaggaact gaggactgat aaaggttttt
     2821 actggctgag ctggaatcag gccgccaact ggtgtctgca acggaatacg aatcttgaag
     2881 aggccttgct ttgggcagac tctgccagca gccggaattt tggaggcgaa tttgtatttg
     2941 ctccctgggt gacgaaagca aagatcctgg agaaactggg gcgtaatgaa gaagcggccg
     3001 ccgtgatgaa aaaagccctt ccgatgggaa gtattattga tatccaccag tatggacgtc
     3061 agttactcag cctgaaacag cccaaagaag cgctggacgt attcaagttg aattttacaa
     3121 aatacccaaa tcagttcacc accttaatgg gactggtacg tggatactcg ggcgttggtg
     3181 attttaaaaa cgcattgaaa tatgcaaatc tggccatccc ccaggctccg gatgaacaaa
     3241 acaaaaagtc cgttcaggga atgattgata aactcaaaga ggggaaagac gtgaattaaa
     3301 aagtaaaaaa taaaaagtaa aaaccgacta aacagaattg ctcaaaccag tatgtaaatg
     3361 acctgaggaa gggctgttta ctcctgacta ccgactcctg actaccgact gattagttac
     3421 atccgagcaa ttcacacaat ttcgcctcca gcgcgggacc ccgtaggttc ttggcaatga
     3481 tttttccttc ggggtccagg agataattct gcggaatact ctgaatccgg tacagccggg
     3541 caactgcatt attccagaat tgcagatcgc ttacatgtgt ccaggtaaga ttgtcctcat
     3601 gtatggcttc tatccatctt tctttttgtc cagggcgatc cagcgaaata cccagcacgg
     3661 taaaattctt tggactgaac tttttgaaat tttctaccac atttggattt tcctggcggc
     3721 aggggccgca ccagcttgcc cagaaatcca ccagcacata tttccctctg
//
LOCUS       DAOPMW010000340         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29205_length_3751_cov_6.019481, whole genome shotgun sequence.
ACCESSION   DAOPMW010000340 DAOPMW010000000
VERSION     DAOPMW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29205_length_3751_cov_6.019481"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLU11_13100"
     CDS             <1..546
                     /locus_tag="PLU11_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPN60115.1"
                     /translation="KTIPVIEALDGQLITNRLDLVPKLANGEIISDTENDVLKIVVVN
                     RYQEAPVAKAFIKGIGLKQGALASSVAHDSHNIVAVGVDDESLCRAVNLVIAERGGLS
                     AAGQGQELVLGLPVAGLMSNGDGYEIADAYTRLDQFTKNELGSILASPFMTLSFMALL
                     VIPHLKLSDKGLFDGDGFRFV"
     gene            587..2509
                     /locus_tag="PLU11_13105"
     CDS             587..2509
                     /locus_tag="PLU11_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60116.1"
                     /translation="MNKSIFLLFILLVFVKHNSWANYFTIITDKSSYKHTETLSYTVF
                     TQLTACQMAQPAAYVYIHSADHVLISRQILQLNQESTIISIPLTGFTNGFYFISVFGY
                     AVGKNSKLDGNTVCIAVDLPDSVTQQLDNRTAELLPAGGNALINFRNRMIVLLKSCGG
                     NPVYDKISFRNRQQQLIAVCQTNRFGWGAVDLPVAPDDSIFITDSKNTLLSVIPVENN
                     RLFSNSGFSLHLPPYNDTMIIQVRRGDAATGHKVYLEVYEKEHAIAELPVRFLNDTSI
                     VNILFPASDFSNKLLKVLLKNEKAEIIAERYIYNENKDHNDSTGLFCEAISGTAPFYL
                     DTGNPALNDGLIAFEPEYRDPANNLAEEGFRLAFSHPDYSKTQIDYYLYNSRHEIIQA
                     GSSFADSSGQIRISGCDFYDSGYVKLYVNKKEVDGFEEVLRGMDPEELRKINARFSQI
                     KNINIGQTIIPSAAQPATVTNFKTLENINLNVIVKNRIPELEKKYISNGLFRDHNSIQ
                     VNVEDDPLALNYSLTEYLVRKIPGLFIYNGELRYRNGYVEIYIDEMYMSNILLNQLIQ
                     TNDIGYIKFFRNPISSGLQATDLGSAAKGNSYAGGIQGSLAIYTRKSATINYNQPFRN
                     GLLVKGYANEEIRPAQ"
     gene            complement(2565..>3751)
                     /locus_tag="PLU11_13110"
     CDS             complement(2565..>3751)
                     /locus_tag="PLU11_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HPN60117.1"
                     /translation="FEADKKKTGKPDWDFLERAFQKLLMNFTWWVNQKDANGTDIFEG
                     GFLGLDNIGVFDRNHMPPGIKKLQQADATSWMAMYSINMLRMSLELAEHNRAYEESAA
                     KFFRHFLNIGWAMHHIGKKDISLWDDKDAFYYDAIQFENGSSERLRIRSLVGIIPMLA
                     VEVMHGGLFEQLKQFNTRLEVIRLTRPDLTRIISDINKKNAEGNYLFAIMIGDRMEHL
                     LKRLLDEAEFLSDYGIRSLSKIHETPYAFSYKGNEYSIQYEPGESSSSMFGGNSNWRG
                     PIWLPLNYLIIHSLRKYYDYYGDNYTHEFPAGSGKKLNLKQIANELTKRLLKLFERNK
                     ERDGAFQYHDQQHPQWSENYFREHHLFYEFFHGDNGKGLGASHQTGWTAMVVNLLLEM
                     EE"
BASE COUNT         1073 a          867 c          831 g          980 t
ORIGIN      
        1 aaaactattc ccgttattga ggccctggat ggacaactga tcaccaacag actggacctc
       61 gtacctaaac tggcaaatgg agaaataata agtgatactg aaaatgatgt gctgaagatc
      121 gtggtggtga accggtacca ggaagcaccg gtggccaaag cctttatcaa aggaatcgga
      181 ttgaaacagg gcgcccttgc ttcttccgtg gcacatgaca gccataatat cgtggccgtg
      241 ggggtggatg atgaaagtct ctgccgggcc gtaaacctcg tgatcgcgga acggggtggt
      301 ctgagtgccg caggccaggg tcaggaactg gtgcttggtc tgcctgtagc cggactcatg
      361 agcaatggcg atggctacga aattgccgat gcctataccc ggctcgatca attcaccaag
      421 aacgaactcg gctcaatcct tgcctcaccc tttatgacgc tatcttttat ggctttactg
      481 gtgatcccgc atttgaagct gagtgataaa gggttgtttg acggggatgg gttcaggttt
      541 gtgtaaaaaa aaaataaata tttgctgagg cgttacataa aaaactatga acaaatcaat
      601 ttttctttta tttatactcc tcgtattcgt caaacataat agttgggcga attatttcac
      661 gattataaca gacaaatcct cctacaagca cacagaaacg ctcagctata cggtattcac
      721 acagcttacg gcttgtcaaa tggcacagcc tgctgcctat gtgtatatcc attctgctga
      781 ccatgttctc atcagcaggc agattttaca actcaaccag gaatccacta ttatatccat
      841 tcctttaacg ggctttacta acgggtttta tttcatcagt gtcttcggct atgctgtcgg
      901 caaaaattca aaacttgacg ggaatacggt ttgtatagcc gttgacctgc ccgattcggt
      961 tacacagcag ttagacaacc gtacggctga gctgctacca gcaggtggca acgcgctgat
     1021 taattttaga aaccggatga tcgtcctact taagtcatgc ggcgggaacc cggtctatga
     1081 taaaatctct ttcagaaacc ggcagcagca attaatcgcc gtttgtcaga ccaatcgttt
     1141 tggctggggt gcagtagatc tccctgttgc tccggatgac agtatcttca taaccgattc
     1201 aaaaaataca ctgctatcgg ttataccggt tgagaataac cgattattca gcaatagtgg
     1261 attttcactt cacttaccgc catacaacga cacgatgatc atccaggtaa gaagaggaga
     1321 tgcagctaca ggacataaag tatacctgga agtatatgaa aaagaacacg ccattgcaga
     1381 actacctgtc aggttcctca atgacacatc gattgtaaac atactctttc cggccagcga
     1441 tttcagcaac aaattgctga aagtcctgct aaaaaatgaa aaggctgaaa taattgctga
     1501 acgttatata tacaatgaaa acaaggacca taacgattca accggattgt tttgtgaagc
     1561 catttccggc actgctcctt tctacctcga taccggcaat cctgctttaa atgacggcct
     1621 gatcgccttt gaaccggagt atcgtgatcc agcgaacaac ctggcagaag aaggtttccg
     1681 gcttgctttc agtcatccgg attacagtaa gacacaaata gattattatc tgtacaatag
     1741 ccggcatgaa atcattcagg ccggcagttc cttcgctgac agttccggtc agatccggat
     1801 cagtggttgt gatttttatg acagtggcta tgtgaaatta tatgtaaata aaaaagaggt
     1861 tgatggattt gaagaagtat tacgcggaat ggatccggag gagctgagaa aaataaatgc
     1921 ccgattcagc cagatcaaaa atataaatat aggccaaact attattccat ctgctgcaca
     1981 gccggcgacc gtgaccaatt ttaaaacgct ggagaatatc aaccttaacg ttattgtcaa
     2041 aaaccgtatt cccgaattgg agaaaaagta tatcagcaat ggactattca gggaccataa
     2101 ttcgatccag gtcaacgtgg aagacgaccc gcttgcctta aactactccc tgactgaata
     2161 cctggtaaga aaaatacctg gtttattcat ttacaacggt gaactaagat acagaaacgg
     2221 ctatgtagaa atctatattg atgaaatgta tatgtccaat atcctcctga atcaattaat
     2281 acagacgaat gatattggct atatcaaatt tttcagaaac ccgatcagca gtggcctgca
     2341 ggcaacagat ttgggaagtg ccgcaaaagg caacagttat gcaggaggta tacagggaag
     2401 ccttgctata tacacaagaa aatcagcgac aataaattat aatcaacctt ttcgaaacgg
     2461 tttattagta aaaggatacg caaatgaaga aatccggcca gcacaataat catacatgga
     2521 tgaccgctaa actccggctt caggactact atctactaac tggtctattc ttccatttcc
     2581 agcaataaat tcaccaccat tgccgtccac cccgtctgat gcgaagcccc caggcccttg
     2641 ccgttatctc catgaaaaaa ttcatagaag aggtggtgtt cacgaaaata attctccgac
     2701 cattgcgggt gttgctgatc atggtactgg aaggccccgt ctctttcctt gttgcgttcg
     2761 aatagtttta acaaacgctt cgtgagttca ttggcgatct gcttcaggtt gagttttttg
     2821 cccgaaccgg ccgggaattc atgggtataa ttgtcaccgt agtagtcgta gtatttgcgc
     2881 agcgagtgaa tgatcaggta attcagcggc agccagatgg ggccgcgcca gttggaattg
     2941 ccgccaaaca tggaactgga actctccccg ggttcatatt gaatactgta ttcattgccc
     3001 ttataactga aggcataagg cgtttcatgt attttggata aggagcggat accataatcg
     3061 gacaagaatt ccgcttcatc aagtagccgc ttgagcaggt gctccatccg gtcgccaatc
     3121 atgatggcaa agagataatt gccctccgca ttcttcttat tgatatcgga aatgatacgg
     3181 gtgagatcag gccgtgtgag ccggatcact tccagccggg tattgaattg tttgagctgt
     3241 tcaaacagtc ccccatgcat cacttccacc gccagcatgg gaatgatccc caccagggag
     3301 cggatcctca ggcgttcgct gctgccgttt tcaaactgga tggcatcata ataaaaagca
     3361 tccttatcat cccagagtga gatatccttc ttcccgatat ggtgcatggc ccatccgata
     3421 ttcaggaagt gccggaagaa cttggccgcg gattcctcat aagccctgtt atgttccgcc
     3481 agttcgagcg acattcgcag catattgatc gaatacatag ccatccagct ggtggcatca
     3541 gcctgctgga gtttcttgat ccccggaggc atatggttgc ggtcaaatac cccgatatta
     3601 tccagaccaa ggaaaccacc ttcaaaaata tccgttccat tggcatcctt ctggttcacc
     3661 caccaggtga aattcatcag caatttctga aaagcccgtt ccaggaaatc ccagtcgggt
     3721 ttacccgttt tctttttatc cgcttcaaac a
//
LOCUS       DAOPMW010000341         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29245_length_3748_cov_3.711887, whole genome shotgun sequence.
ACCESSION   DAOPMW010000341 DAOPMW010000000
VERSION     DAOPMW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29245_length_3748_cov_3.711887"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU11_13115"
     CDS             <1..886
                     /locus_tag="PLU11_13115"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPN60118.1"
                     /translation="LNGNGEMTGTTWITESGFLETPVMITNTNSVGVVRDAVLKWFVK
                     TGWYKEDFWYTYPVVAETYDGFLNDIYGFHVKEENAWEALDSAKSGPLKEGNVGGGTG
                     MMCLGFKGGTGTSSRVFKVKDSTYTLGVLVQANFGAKRNLTIAGVPVGRELKDTLNYE
                     LKAPPAYNPGDGSIIVVLATDAPLLPHQLKRIAARIPLGIGIVGGRGENGSGDIFIAF
                     STANPAAFQREDFTKVDELPNDLINPLFDATVQATEEAIINAMVAAGTMEGINGNKAY
                     ALPHQKVMELLRKWGRVK"
     gene            964..1416
                     /locus_tag="PLU11_13120"
     CDS             964..1416
                     /locus_tag="PLU11_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN60119.1"
                     /translation="MVKYIVLTLMVTVQFFSAVSQSKVSAKELKVQNTIIALFQSLAD
                     RDYEKMMENCTADFTVLENGVIWNLDTLQEKISLSKAIPDFNRVNTIEFTDSRVKGKT
                     AWVSYHNRADYIRNGKKSYVQWLESVVLVKEGRKWRVQLLHSTVVKRG"
     gene            1580..3085
                     /locus_tag="PLU11_13125"
     CDS             1580..3085
                     /locus_tag="PLU11_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPN60120.1"
                     /translation="MKAVTLLLSLTLTLLLAGCKKKSTPDPATEPDQSSFASASVINQ
                     RLGRGINLGDTYEASWAEKVADPADFQRIAASGFKHVRIPVRWEMPTRSTYSSPYIIQ
                     PAFLQSVKKAVEAALKNKLHVIINMHHHDSLFKNPDGLKPMFLAQWKQIASYFKQYSD
                     SLLFEVLNEPHDQLDADKWNVFFADALTEIRKTNPKRTVLLGVAEWGGVSALNKLLIP
                     SDTHLILTIHYYNPFTFTHQGAEWVSGSNPWLGTKWYDTEYDREAVVNDFQAVLRIQQ
                     EKNIPVHIGEFGAYSKADMNSRVKWTRFLSRWFEQNGFSWAYWEFNSGFGVYDPVTGQ
                     YRTALLNALTTEAMTSPTPVNLIDLYSSNFSTSLDGWNLYNNDASAASVLSLVSNRAQ
                     VAITSPGNQNWHIQLIRPGITIQSGKTYRVSFNAYSTGSRSLSASLMLNASPWTLYSY
                     ADFTISSTDKLYSYIFTATATDPAAAFVFSMGNAGVLPVTLYNIKFQEVQY"
     gene            3154..3543
                     /locus_tag="PLU11_13130"
     CDS             3154..3543
                     /locus_tag="PLU11_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HPN60121.1"
                     /translation="MEPSTTAKIINYFIATVWLVNGLFCKILNLVPRHQEIVANILGQ
                     EHSRFLTIAIGISEVLMAVWILSGIRSRLNTILQIVVIAVMNTLEFILVPGLLLWGRF
                     NSLFALLLILVIYYHEFHLKPKITPHS"
     gene            3543..>3748
                     /locus_tag="PLU11_13135"
     CDS             3543..>3748
                     /locus_tag="PLU11_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HPN60122.1"
                     /translation="MFSFLKDHPFAVEAFFESSLVLTFAVPKEQLEPYIPECLQLDTF
                     QDRWAFIAIAMVQTKDLRPKGFPR"
BASE COUNT         1003 a          778 c          946 g         1021 t
ORIGIN      
        1 cctcaatggc aacggcgaaa tgacgggcac cacctggatc accgaatccg gtttcctgga
       61 aaccccggtt atgatcacca ataccaatag tgtgggtgtg gtaagagatg cagtactgaa
      121 atggtttgta aagactggct ggtacaaaga ggatttctgg tacacctatc ctgtggtggc
      181 cgaaacctat gatggttttc tcaatgacat ttatggattt catgtaaagg aggaaaatgc
      241 ctgggaggcg ctggacagtg cgaaaagcgg tccgcttaaa gaaggtaatg tcggcggtgg
      301 tacggggatg atgtgtcttg gttttaaagg cggtaccggc acttcatcca gggtttttaa
      361 agtaaaagat tcaacttata cattgggggt tttagtacag gccaattttg gcgccaaacg
      421 aaacctgacg attgccggcg tgccagtggg tcgggaatta aaagataccc tcaactatga
      481 actgaaagcc ccaccagctt ataatcccgg agatggttct atcattgtag tactggctac
      541 cgatgcgcct ttattaccgc accagttaaa gcggattgcg gccagaattc cattgggtat
      601 tggtattgtg ggaggccggg gagagaatgg ctcaggcgat atctttattg ctttctccac
      661 cgcaaaccct gcggcttttc aacgggagga ttttaccaaa gtggatgagt tgcccaatga
      721 tttgatcaat ccgttgtttg acgccacggt gcaggcaacg gaagaagcta taattaatgc
      781 gatggtggct gccgggacca tggaaggcat caatgggaat aaggcgtatg cattgccaca
      841 tcagaaagtg atggagttgt tgaggaagtg gggaagagtg aaatgagtcg gtagtctgca
      901 gtcggtagtt agtagtcaac agcccatcct gtgagtgtta gtttaaaata tgaaggtttt
      961 attatggtga agtatatagt actgactttg atggtgactg ttcagttttt ttccgcggta
     1021 tcccaaagta aggtgtcagc gaaggagttg aaagtgcaga atacgattat tgctctgttc
     1081 cagtcattag cggaccgcga ttatgaaaaa atgatggaaa actgcaccgc ggattttact
     1141 gtattggaaa acggggttat ctggaacctg gatactttac aggagaagat cagtctcagc
     1201 aaagccattc ctgattttaa ccgggtcaat acgattgaat ttaccgatag ccgggtaaaa
     1261 ggaaagacag cctgggttag ttatcacaac cgggcagatt atatccggaa cggaaaaaag
     1321 agttatgtgc aatggctgga gagtgtggtt ttggtgaaag agggaagaaa gtggagggta
     1381 cagttgttgc attccacggt agtgaagcgg ggatagccct gagggacttc aacagtataa
     1441 atctataagc ggtggaatac gcttatatga aacaaagttc cattgtattc atctgcgtag
     1501 tctgcgtact gatctgcgcc attctgcggg aaatggctgt tcacttgaat ttccatagat
     1561 tatagattaa ctttacgcta tgaaagcggt aaccctactc ctttccctga ccctcacact
     1621 gttactcgcc ggttgcaaaa agaaaagcac tcccgatcct gccactgagc ccgatcagtc
     1681 ttcttttgct tccgcttcgg tcattaatca acggctgggc cggggtataa atcttggtga
     1741 tacgtatgaa gccagttggg cggaaaaagt ggcagatccg gccgattttc aacggatcgc
     1801 tgccagtggt ttcaaacatg tacgtattcc ggtacgttgg gaaatgccca cccgtagtac
     1861 ttactcgtct ccgtatatca ttcagcctgc atttttacag tccgtaaaga aagccgtgga
     1921 agcggccctg aagaataaac tccatgtgat catcaatatg catcaccatg acagtctgtt
     1981 caaaaatcct gatgggttga agcccatgtt cctggcccag tggaaacaga ttgcttccta
     2041 ttttaaacaa tactccgaca gtcttttatt tgaagtgctc aatgaaccac atgatcagct
     2101 ggatgcggat aaatggaatg ttttttttgc cgatgcactc acagagatcc ggaaaacaaa
     2161 tccgaaacgt accgtattgc tgggcgtggc cgaatggggt ggggtatcgg ccctgaataa
     2221 actgctgatt ccatccgaca cacatttaat cctgacaatc cattattata atccgtttac
     2281 gtttacgcac cagggggctg aatgggtgag tggctctaat ccctggcttg gaacgaagtg
     2341 gtatgatacg gagtatgacc gggaagcggt ggtgaatgat ttccaggccg tactccggat
     2401 acagcaggaa aagaacatac ccgtgcatat cggggagttt ggtgcgtata gtaaggccga
     2461 catgaattcg cgggtaaaat ggacgcgttt cctatcgcgt tggtttgaac agaacggatt
     2521 cagctgggcc tactgggagt tcaattccgg tttcggggta tacgacccgg ttacaggtca
     2581 gtatcgtact gccttgctga atgcccttac tacagaggca atgacaagtc ccaccccggt
     2641 gaacctgatc gacctctaca gcagtaattt cagtacctca ctggatgggt ggaatctcta
     2701 taacaacgat gcatcggctg cttctgttct gagtttagtt tcaaacagag cccaggtggc
     2761 aattaccagt ccgggcaacc agaactggca tatccagtta ataagacccg gcattacaat
     2821 acaatcgggc aaaacctacc gcgtgagttt taatgcttat tctacaggca gccgtagttt
     2881 atccgcctcg ttgatgctga atgcatcccc atggactttg tatagctatg ctgactttac
     2941 tatttcatct actgataagt tgtatagtta tattttcacg gctacggcca ctgacccggc
     3001 cgctgccttt gtattttcga tggggaatgc tggggtactg ccggtgacac tgtataatat
     3061 taagtttcag gaagtgcaat actgagcaga aaatgagaaa taaagaatga ggaataagga
     3121 ataaggaagt ttgctactcg ccggtactta gttatggaac cttcaactac agctaaaata
     3181 ataaattact tcattgctac tgtctggctg gtcaatggtt tattttgtaa aatattgaac
     3241 ctggttccac ggcaccagga aattgtagcc aatatacttg gacaggaaca ttcccggttt
     3301 ttgacaatag caatcggtat cagtgaagtg ctgatggctg tatggatttt aagcgggatc
     3361 agatcaagac tgaataccat tctccagatc gtcgtgatcg cggtaatgaa tacgctggaa
     3421 tttatactgg taccgggact cctcttgtgg ggaagattca attcactttt tgctttactg
     3481 ctgatacttg taatatatta ccatgaattt cacctgaaac ccaaaatcac cccgcattcc
     3541 tgatgttttc ctttttgaaa gatcatcctt ttgccgtgga ggcttttttt gaaagttcgc
     3601 tggtgctgac ttttgcggta cctaaggaac agctggaacc ttatattccg gaatgcctgc
     3661 aactggatac tttccaggat cgatgggctt ttattgccat tgccatggta cagacaaaag
     3721 acctgcgtcc gaaaggcttc ccgcgatt
//
LOCUS       DAOPMW010000342         3739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29352_length_3739_cov_5.357763, whole genome shotgun sequence.
ACCESSION   DAOPMW010000342 DAOPMW010000000
VERSION     DAOPMW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29352_length_3739_cov_5.357763"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..680)
                     /locus_tag="PLU11_13140"
     CDS             complement(69..680)
                     /locus_tag="PLU11_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60123.1"
                     /translation="MKPLILISAATLLVFTSCTSTKIVSSWREPDKQVTVDKLNKVLV
                     LALFKDETSRYKAEDQMVQYLDGKGVASYSYLKTSFNENNEEALRRKIKNDGFDGAVT
                     MRLVDVDKEKTYTPGIGSSYPNYYRNFSGYYYRSWSYFSNPGYYSTTKTYTVETNVYS
                     IKEDRIIWTGLTETTDPDGVDKMTAEITNLIKKEMIKEGCINR"
     gene            912..1412
                     /locus_tag="PLU11_13145"
     CDS             912..1412
                     /locus_tag="PLU11_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HPN60124.1"
                     /translation="MTTMIKTTNKMKAEIGVTGLNLKAVANELNVILADEYVLYTKTK
                     NAHWNVEGPDFFEKHTFFEGQFTQLDDIIDKVAERIRTIGHYAAGSLKLYLELTQLTE
                     SGDKNDSQGFIKELLMDHETIIIRCRENIHRFAEEYKDAGSSDFVTGIMEVHEKMAWF
                     LRAHLD"
     gene            1424..2533
                     /locus_tag="PLU11_13150"
     CDS             1424..2533
                     /locus_tag="PLU11_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HPN60125.1"
                     /translation="MKQQNIFDPLFIGEGTLHNRIVMAPMTRCRATGNIPNKMMAEYY
                     RQRSGAGLIITEGTSPSANGLGYARIPGIFTSQQEEGWNKVTQAVHGNGGKIIIQLMH
                     CGRISHPLNMPEGSRIIAPSAVQPAGKMWTDAKQLQDYPVPEVMTEDDISTVIVEYVA
                     AAKRAIAAGFDGVELHGANGYLLEQFLSPVTNIREDGYGGSITNRCRFVLEVVDAVSA
                     AIGKDKTGIRLSPYGVAGDMSPYPETDPTYQHLSKKLNKIGISYIHLVDHTSMGAPVV
                     PFLIKKMIRKNFSKTLIVCGGYDLERARDVIEDGMADLVAFGRPFINNPDLVERFINH
                     WPLEKELKTDLFYTAEDKGYTDYPVHQEEPVAAGI"
     gene            2557..2796
                     /locus_tag="PLU11_13155"
     CDS             2557..2796
                     /locus_tag="PLU11_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60126.1"
                     /translation="MATYKSIMADILSTTMRIQEQFPELTKFMPEMPVAHPVNSDQEI
                     TIKSLKEYRDSLKKLLENYSTQHEAEPLPEKNNLT"
     gene            2793..3296
                     /locus_tag="PLU11_13160"
     CDS             2793..3296
                     /locus_tag="PLU11_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN60127.1"
                     /translation="MKKILIALDYDPSAQKVAETGVSLCSESGDVGTLLHVIDSPAYY
                     VSEGYNPIMGFTGYMNPEQTGPLELDQILGGTEKYLQKTRQHLGHPEMRTLVKEGDPG
                     KIIISTAEEMKVDLIIMGSHSRRWLEMIPLGSITTYVLHHTRIPVLIVPTGKEGQQTG
                     SEAATLP"
     gene            3430..>3739
                     /locus_tag="PLU11_13165"
     CDS             3430..>3739
                     /locus_tag="PLU11_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60128.1"
                     /translation="MAVLLLSACAKKITLNTSSKVPAARGFIKVKKDKNSNYAIDVKV
                     NNLAEAERLQAGKKVYVIWLVSNNEYPKNVGLINSSTKQFSKKLTADFHTVSSAKPDK
                     V"
BASE COUNT         1111 a          724 c          882 g         1022 t
ORIGIN      
        1 taaacgtcca tttttatggt aaaaatatat tatgtgtaat atggctaaaa aatgattgcc
       61 atgatgattt accgattgat acagccttct ttaatcattt cttttttaat caaattagtt
      121 atttctgctg tcattttatc gactccgtct gggtcagtgg tttcagttaa tccggtccag
      181 ataatccggt cttctttgat ggaatacaca ttggtttcaa cggtataagt ttttgtagtg
      241 gagtaataac ccgggttgga aaaataggac caactgcggt aatagtagcc actgaagttg
      301 cggtagtagt tgggatagga agagccgatg ccgggcgtat aggttttttc cttgtccaca
      361 tccacgagcc gcatcgtcac tgctccgtca aagccatcgt tttttatttt tctccgcagg
      421 gcttcttcat tgttttcatt gaaactggtt ttgagataac tataggaggc cactccttta
      481 ccatccaggt attgtaccat ctggtcttcc gctttatagc ggcttgtttc gtccttgaat
      541 aaagccagta caagtacttt attcagtttg tcaacggtca cctgtttgtc aggttctctc
      601 cagctactga caattttggt agaggtacag gaagtaaaaa caagcagcgt ggctgctgaa
      661 ataaggatca acggtttcat aaaaatttgt tttaaacgat gttgccggga aaaagaaatc
      721 gatctatgca aaattgatca atctgagcca gccaatactt ataccacttt gccggaatgt
      781 tgcagatttc cgggttattt tttttccatc cggtttaaac aagtgcttct cagaggtata
      841 cctgtgaaag atgatactat ttgtgaatac catacaatac gaaaccaacg ctttagatcg
      901 aactttatag tatgactaca atgattaaaa caacaaataa aatgaaagca gaaattggag
      961 ttaccgggct taacctgaag gcggtagcta atgaactaaa tgtaatcctg gcagatgagt
     1021 atgtgcttta taccaaaaca aagaatgcgc actggaatgt ggaagggcct gacttttttg
     1081 agaagcatac tttttttgaa ggtcagttta ctcaacttga tgacattatt gataaagtgg
     1141 cggaacggat ccgtacgatc gggcattatg cggcgggttc actgaagtta tacctggaac
     1201 taacccagct gaccgagagt ggtgataaga atgacagcca gggatttata aaagagttac
     1261 tgatggatca tgaaactatc attatccgct gccgggaaaa tattcaccgg tttgcggagg
     1321 agtataaaga cgcgggcagc agtgattttg taaccggtat tatggaagta catgagaaga
     1381 tggcctggtt tttacgggca cacctggatt aaaagacgga agaatgaaac agcaaaacat
     1441 atttgatccc ttattcattg gcgagggaac tttgcataac cggatcgtca tggctcctat
     1501 gacccggtgc cgggcaacag gtaatattcc caataaaatg atggctgaat attaccggca
     1561 acgatcagga gccggtttaa taatcactga gggcacttca ccttctgcaa atgggctggg
     1621 ttatgcacgt ataccgggga tttttacttc ccaacaggag gaaggctgga ataaggttac
     1681 ccaggccgtg catggaaatg gaggaaagat aattattcag ttaatgcatt gcggccggat
     1741 cagtcatccg ttgaatatgc cggaaggcag ccgtattatt gctccatctg ccgtacaacc
     1801 cgcgggtaag atgtggaccg atgcaaagca gttacaggat tatcctgtac cggaagtaat
     1861 gactgaggat gatatttcaa cagtcattgt agaatatgtg gctgccgcca agcgggctat
     1921 tgccgccgga tttgatgggg tggagcttca tggggccaat ggatatttgt tggaacaatt
     1981 tctctcaccg gtcactaata tccgtgaaga tggatatgga ggcagtatta cgaaccgctg
     2041 ccggtttgta ttggaagtgg ttgatgcggt ttctgctgcg atcgggaagg ataaaacagg
     2101 catccgttta tcaccttatg gagtggcggg tgatatgtca ccctatccgg aaacagatcc
     2161 cacttaccag cacttgtcaa agaaattaaa caagattggt atttcctata tccacctggt
     2221 ggatcatact tccatggggg caccggtagt tccgtttctt attaaaaaga tgatccgcaa
     2281 gaacttttcc aaaacgctga tcgtttgtgg agggtatgac cttgaaaggg cgagggatgt
     2341 aattgaagat ggaatggcgg acctggtggc ttttgggagg ccgtttatta ataaccctga
     2401 cctcgtggaa agattcatca atcattggcc cctggaaaaa gagttgaaaa cggatttatt
     2461 ttatacggcg gaagataaag ggtatactga ttatccggta catcaggagg agcccgttgc
     2521 ggcaggcatc tgaatagtat cattaattaa aaaattatgg caacttacaa aagcataatg
     2581 gcggatattt taagcactac catgcggata caggaacaat ttccggagtt aacgaagttt
     2641 atgccggaga tgcctgttgc ccatcctgta aacagcgatc aggaaataac gattaaaagc
     2701 ctgaaggaat atcgtgattc attaaagaag ctgttagaaa actattccac acaacacgag
     2761 gctgaaccgt tgcctgaaaa aaataattta acatgaaaaa aatattaatc gcgttggatt
     2821 atgatccaag tgcacaaaaa gttgctgaaa ccggggtgtc gctgtgcagt gaatccggtg
     2881 atgtggggac gttgcttcat gttattgata gtccggccta ttatgtatcg gaaggttaca
     2941 atccgattat gggctttacc ggctatatga atccggaaca aaccggacct ctggaacttg
     3001 atcagattct cgggggcacg gagaaatacc ttcaaaagac aaggcaacac ctgggacatc
     3061 cggagatgag aaccctggta aaggaaggtg atcccggaaa aattattata tcgacagccg
     3121 aagagatgaa ggtggacctg attattatgg gatcccacag ccggcgatgg ctggaaatga
     3181 taccgttggg tagcatcaca acatatgtat tgcatcatac ccgtattccg gtattaattg
     3241 tgccaaccgg aaaggaaggg cagcagaccg gtagcgaggc tgctactttg ccgtgatgtc
     3301 ctcttacttt caggtcggaa acagatcata caaatgttat tcatcaaaca acaattttat
     3361 tcattttaaa tacaatttat gcattcagtt accttaaagc gaacaattgc agcaggaatt
     3421 ttcctttcaa tggcagtgct cctgctcagt gcctgtgcca aaaaaattac cctcaatact
     3481 tcttctaaag taccagccgc ccgtggattt atcaaggtaa aaaaagacaa gaattctaat
     3541 tacgcaatcg acgtgaaagt caataacctg gccgaagcag aaagattaca ggccggaaag
     3601 aaagtatatg tgatctggct ggtttcaaac aatgagtatc cgaagaatgt tggcctgatc
     3661 aatagttcaa cgaagcaatt ttccaaaaag ctgaccgccg attttcacac ggtatcttcc
     3721 gcaaagcctg acaaagtat
//
LOCUS       DAOPMW010000343         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29415_length_3734_cov_9.360968, whole genome shotgun sequence.
ACCESSION   DAOPMW010000343 DAOPMW010000000
VERSION     DAOPMW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29415_length_3734_cov_9.360968"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLU11_13170"
     CDS             <1..239
                     /locus_tag="PLU11_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60129.1"
                     /translation="NKRISLEKYNFIKANLLAVLKKTELTHTDLMEALYQRVKDEFEG
                     GVQWYGETVKLDLEARKQIERTGTKPEKYKLKAK"
     gene            273..644
                     /locus_tag="PLU11_13175"
     CDS             273..644
                     /locus_tag="PLU11_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HPN60130.1"
                     /translation="MLTFNLRFFLLSILFFVIEVLIALYVKDDFVRPYVGDYLVVMLI
                     YCAVRTFIKANPVKVAIAVLLFAYMIELLQYFRIVDRLGLSGNQVAKTVIGYGFEWWD
                     MLAYTLGVLTIVLMEKRPKKN"
     gene            680..1759
                     /locus_tag="PLU11_13180"
     CDS             680..1759
                     /locus_tag="PLU11_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HPN60131.1"
                     /translation="MNQRIIGFDLARAYAIFGMFIVNFNFSFGSVMAPSGLTGRFLNL
                     FTGNSTAIFIICAGMGVALMTNKPVYSKEEKAVLKSRILKRSWFLFVLGLLLFNWWSG
                     DILHFYGGYMHVAAFLLFVPKRFYLWGALVAIVVFHLLLLLLPIETSWDFSNFSYRDF
                     WTAPGFLRNTFYNGWNSLFPWIAYFFLGMWLGRLNWQDSLVRRKVFFTGLIVFILLQG
                     LRLLAKEQLFSQYWTDYILAEYFPPYLPFVLLTMAFACMVIPVCMYIGEKYAKNPVVL
                     ALQQTGQMTLTHYVMHLTLGMILMAAMTGKHYTGFLEDEPPTSPFYILGYSVLFYLFS
                     ILFSLLWRKQFRNGPLEMLMRKISG"
     gene            1946..3715
                     /locus_tag="PLU11_13185"
     CDS             1946..3715
                     /locus_tag="PLU11_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPN60132.1"
                     /translation="MNYTLKDGLVVISCLLFFGCGMNKPPLVQAQDAPTEKSPPKKIP
                     IVQKLRQNDHLPVEERIKLYHQLKKEAPQLYSFEDETELTMYGYSILWANKTTEAIEV
                     FKLIATEFPNSSNAYDNLGEGYLKNGNKELALLNYERSLAMDPENFNAEDQVANIRFP
                     DRVPLQPAELFKKVYPATAYRDDLDQLGTTLLKVHPNALKFITREAFWKSIEDKKRQI
                     TDRTTYGAFAWYCSEIIANVHCSHTNMGSFDYEAAMLPESARFPLQTRWISNQLFVID
                     PLSNNKTVKIKDEVVSINGKAVAEIIKECYKHIPAQGYIETTKNHFFNTWSTAMIPFA
                     LGFPDRYEIIIKGTNAPIILNASANIEEPYFDRTLDLSGNNLSLSFIEGNTIAFLKVA
                     SFNYYRWADFEVFKAFIDSSFSEIRKKDVKNLVIDLRQNRGGSQSSSIHLLQYLVNQP
                     FTYYSNAQFEGKRDKIEGEDIVYPFTNRFDGKTCFIIDGVGNSTTGHFMSIARYLKLG
                     TIVGEELGSNQFCSAGMTTRRLANTKLVYYVANNTHESLAVDLPDETGILPDYTVTQG
                     IDDFLIKRDAVKAFAIQLAKQNK"
BASE COUNT         1028 a          740 c          859 g         1107 t
ORIGIN      
        1 ccaataagcg catttcgctg gagaaataca attttataaa agcaaatctg ctggccgtat
       61 taaagaaaac ggagttgacc cataccgatc tcatggaagc cttataccag cgggtgaagg
      121 atgagtttga aggcggggta caatggtatg gggagacggt caagctggat ctggaagcga
      181 gaaagcagat tgaacggaca gggacaaagc cggagaagta caaactcaaa gcaaagtaaa
      241 aaaagattgt tctgtttatt aatttttaag ttatgctgac ttttaatctc cgcttcttcc
      301 tgctcagcat cctgttcttc gttatagaag tattgattgc cttatatgtg aaggatgatt
      361 ttgtccggcc ttatgtgggc gactacctgg tggtgatgct gatctattgt gcggtgcgga
      421 cttttatcaa ggccaatcct gttaaagtag ctattgccgt tctgttattt gcttatatga
      481 tagaactact ccagtatttc cggattgtag atcgcttagg cttgtcgggc aaccaggtag
      541 ccaaaacagt aatcggctat gggtttgagt ggtgggatat gcttgcctat acacttggtg
      601 tgttaacaat agtcttaatg gaaaagcggc ctaaaaaaaa ttagccagca gaccataaca
      661 tgattgtaat ttcggacaaa tgaaccaacg gatcatcgga tttgacctcg cccgggccta
      721 tgccattttt ggcatgttta tcgtaaactt caacttcagt tttggttcgg tgatggcacc
      781 ttccggttta accggccgtt ttcttaacct ctttaccggt aattccaccg ccatctttat
      841 tatttgtgcc ggcatgggag tggccctgat gacgaataag cccgtataca gcaaggaaga
      901 aaaagcagta ctgaaatcca gaatcctcaa acggtcctgg tttttatttg tgctgggttt
      961 attactgttc aactggtggt cgggggatat cctgcatttt tatggagggt atatgcatgt
     1021 ggctgccttc ctgttgtttg ttccgaagcg tttttatttg tggggtgcat tggtcgctat
     1081 tgtagtattt cacctgctct tgttgctgtt accaattgaa accagttggg atttttcgaa
     1141 tttcagttac cgggatttct ggacagcacc cggttttctg cgcaatacat tttacaatgg
     1201 ctggaactcc ctgtttccct ggatcgctta ttttttcctg gggatgtggc tgggaaggct
     1261 caactggcag gatagccttg tacgacggaa agtgtttttc acgggcttga ttgtttttat
     1321 attgttgcag ggactccgcc tgctggccaa agagcagttg ttcagtcaat actggacgga
     1381 ttatatcctg gcggaatatt tcccgcccta tttacctttt gtattgctga ccatggcttt
     1441 tgcgtgcatg gtaattccgg tttgtatgta tatcggtgaa aaatatgcga aaaacccggt
     1501 tgtgctggct ttgcagcaaa ccgggcaaat gacgttgacg cactatgtca tgcaccttac
     1561 cctgggaatg atcctgatgg ccgcgatgac aggcaaacac tacaccggct tcctggaaga
     1621 tgaaccccct acttctccct tttatatcct tggttactcc gtgctttttt atctattcag
     1681 tattttgttc agccttttat ggagaaaaca attcaggaac ggtccattgg aaatgttgat
     1741 gcggaaaata tcgggatagg aattgaataa ggtccgggtt agcggtatcc ggaatggtaa
     1801 gttccttttt ttagcttaaa tctgatttat attcgactaa agcatagtgg tttacattaa
     1861 atttaccatg ttttacactg ttttacacgg tctatactgc aaactatacg caacagatta
     1921 tctttaaaaa aatggtatgc agtttatgaa ttacacactt aaagatggtc tggttgttat
     1981 tagttgcctt ttgttttttg gctgcgggat gaataagccg ccactcgttc aggcacagga
     2041 tgccccaaca gaaaaatcgc cccctaaaaa aatacccatt gttcaaaagc tcaggcaaaa
     2101 tgatcactta cctgttgaag aacggataaa actgtaccac cagttaaaaa aggaagcccc
     2161 tcaactttac agtttcgagg atgaaaccga actgaccatg tatggctatt ccattttgtg
     2221 ggccaataaa acgacggaag ccattgaagt gtttaaactg attgcgactg aattccccaa
     2281 ttcctcaaat gcgtatgata acttagggga aggatatctg aaaaacggga acaaggaact
     2341 ggccctgttg aattatgaac gttcactggc catggatccg gaaaatttca acgcagaaga
     2401 tcaggtggca aatatccgtt ttcccgacag ggtgccgctt caacccgctg aattgtttaa
     2461 gaaagtctat ccggccactg cttaccggga tgatctggat cagttgggaa ctaccttgct
     2521 aaaagtacat ccgaatgcgc tcaaatttat tacccgtgag gcattctgga aatcaattga
     2581 agataagaag cggcaaatca ctgatcgtac cacttatgga gcatttgcct ggtattgcag
     2641 tgagatcatt gctaatgtac attgttcgca taccaatatg gggagttttg attatgaagc
     2701 ggctatgctg cccgaatccg cccgctttcc actgcaaacg cgttggatca gcaaccagct
     2761 gtttgtaatt gatccattga gtaataacaa aacagtaaaa attaaggatg aggtagtgag
     2821 tataaatggt aaagcagttg ctgaaattat caaagagtgc tacaaacata ttcctgcaca
     2881 aggttatatc gaaaccacca agaatcattt ttttaatacc tggagtacag ccatgattcc
     2941 ctttgccttg ggatttcctg acaggtatga gattattata aaaggaacaa atgcgcccat
     3001 tatcctgaat gcatcagcaa acatagagga gccctatttt gacagaaccc tggatctttc
     3061 tggtaacaat ctttcactca gttttataga ggggaatacg atcgcttttc ttaaagtggc
     3121 ttcatttaat tattaccggt gggctgactt tgaagtgttt aaggctttta ttgacagtag
     3181 ttttagtgaa atccgaaaga aagatgttaa aaaccttgtt attgacctgc ggcaaaacag
     3241 gggcggttcc caatcctcca gtattcattt attgcagtac ctggtgaatc aacccttcac
     3301 ctattattca aatgcccagt ttgagggcaa gcgggataaa attgaagggg aggatattgt
     3361 atatccgttt acaaaccggt ttgacgggaa aacctgcttt ataattgatg gagttggcaa
     3421 ctcaacgaca ggtcacttta tgtccattgc ccggtatctg aagctgggaa cgattgtggg
     3481 ggaagagctt ggttccaatc agttttgttc ggccggcatg acgaccagga gactggccaa
     3541 tacaaaattg gtctactatg tggcgaataa tacccatgag tcgttggcgg ttgacctgcc
     3601 ggatgaaacc ggaattttac cggattatac agttacacag gggatagatg atttcttaat
     3661 taaaagggat gccgtaaaag cttttgcaat ccaactggct aaacaaaata agtaagcaac
     3721 gaatccgtta ttca
//
LOCUS       DAOPMW010000344         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29650_length_3713_cov_9.080098, whole genome shotgun sequence.
ACCESSION   DAOPMW010000344 DAOPMW010000000
VERSION     DAOPMW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29650_length_3713_cov_9.080098"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLU11_13190"
     CDS             <1..490
                     /locus_tag="PLU11_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPN60133.1"
                     /translation="IINEILINACQAPNHGRTEPWQFTVFSGEGLTMLAEFQSNLYKD
                     LAGENFKEATYAKLKQQPLKASHIIAIGMKRSSLKNIPEIEDIEAVACAVQNIYLSVT
                     AYGLGGYWTTGGITYKEEAKSFFGLDEADKLLGFFYIGQVAVPSVGGSRSPLEQKVNW
                     IK"
     gene            495..926
                     /locus_tag="PLU11_13195"
     CDS             495..926
                     /locus_tag="PLU11_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPN60134.1"
                     /translation="MVKQPVTPPYHIYYPVKVAKSKIAGKGAYALQRIPARKKIGDLG
                     GVIITMKEAMRLIKNLKVINLVELDNNLALNASANPNDMRFINHSCGPNTYLRVMKDR
                     VEFYALRDIKKGEELSCDYGETHHEGKLPCKCGAKNCRGFI"
     gene            993..2057
                     /locus_tag="PLU11_13200"
     CDS             993..2057
                     /locus_tag="PLU11_13200"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPN60135.1"
                     /translation="MKKLSVLAFIAMGFMICISFTNQSLRHQYSRPAAEWPAPRVDEG
                     VSWTELGALPPVPVDLTNDSIKNIVELGKALFFDPRLSSSGKISCATCHDPALNWTDA
                     KPRSVGHEGAMGKRNAPTIQNSWFYKKLFWDGRSRDLQDQAFAPIVSESEMNSEMHDV
                     VRKLRRINGYTEWFKKAFGSEHINPHLMTEAIAVFEKSISSGESRFDRFIKGDHRALN
                     KSELRGLHLFRTKAGCFNCHNGPLFTDNQFHRLGAVINNPYDTKDVGLYKVTHLDADT
                     GKFRTPSLRDVMRTGPWFHDGSENNMDSLIFRFHLNSPGPNGVFRPLNLSRKEQKDLR
                     AFLEALSAPPVPFNRPALPQ"
     gene            2122..2598
                     /locus_tag="PLU11_13205"
     CDS             2122..2598
                     /locus_tag="PLU11_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HPN60136.1"
                     /translation="MKKFLLTLFVAGFGFIANAQPPEGEAKPGEWYGDKVTTEGAVNL
                     TDVVAKLNGGAEFPEVKIQAKIVEVCPKKGCWLKLELANGETAMVKMKDYGFFLPLAA
                     KGKTVIIDGKVELKTTSVAELKHYAEDAKKSKEEIDAITQPVKEVKVLAKGIVVVK"
     gene            2761..>3713
                     /locus_tag="PLU11_13210"
     CDS             2761..>3713
                     /locus_tag="PLU11_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN60137.1"
                     /translation="MKTTTLLIAILFLLVSGIRAQETETTLNGTVKTSDGKPASMVSV
                     ILKELKKGVITDEIGQFQFKGIKEGRYTIIVSFVGLETVESQVNFNGKDNRIFDFVLR
                     ENHNELEEIVITSGYTSNEKKMTTGKINIRLMDLPQAVTVISKEAIERQQALHLSDVL
                     QQVNGVYLMGNTGGFQEEIAARGFSFSSSNTFKNGVRFNNAIMPEMSSIEKLEFLKGG
                     SAILFGNVAPGGIMNIVTKKPKFEQGGELSFRTGSYGFYKPSLDVYSGFGKKKQAAFR
                     VNTVYEKAQSFRDYVSSERYYINPSLLFNLSSKTELLIQGD"
BASE COUNT         1102 a          804 c          882 g          925 t
ORIGIN      
        1 catcatcaat gaaatactga tcaatgcctg tcaggcaccc aatcatgggc gtactgaacc
       61 ctggcagttt accgtattca gcggggaggg cttgacaatg cttgcggaat tccaaagcaa
      121 tttgtataaa gacctggcgg gtgaaaattt taaagaagcc acgtatgcca aattaaagca
      181 acagccatta aaagcctccc atattattgc catcgggatg aagcgttctt cgttgaagaa
      241 tattccggaa attgaagaca ttgaagcggt agcctgtgcc gttcagaata tctatttatc
      301 agtgactgct tacgggcttg gtggctactg gaccaccggg ggaattacct ataaagaaga
      361 agctaaatcc ttttttggtc ttgatgaagc cgataaactg cttggatttt tttatattgg
      421 acaagtcgct gttccttccg tgggaggcag ccgcagtccg ctggaacaaa aagtgaactg
      481 gataaaataa gattatggta aaacaacctg ttactcctcc ctatcatatt tattacccgg
      541 taaaagtggc aaaaagtaaa attgccggga aaggggccta tgccttacag cgaattcctg
      601 cccgtaagaa aattggcgac ctgggtggtg tgatcatcac gatgaaagaa gcaatgcggc
      661 tgatcaaaaa cctgaaagtg atcaacctgg tggaactgga caataacctg gcgctgaatg
      721 cctctgccaa tcccaatgat atgcgcttta tcaatcactc ctgtggtccg aacacctatt
      781 tacgggtaat gaaagaccgg gtggagtttt atgcccttcg ggatattaag aaaggagaag
      841 aactgagctg cgactatggc gaaacgcacc atgaaggcaa actcccttgc aaatgcgggg
      901 caaaaaactg ccgcggcttt atctgatctt gtttgtaatc aatatcgtta ttcattcaga
      961 caaaatttgc tttcttcacg ctttattgca atatgaaaaa attatcagtg ctggctttta
     1021 tagcgatggg gtttatgatt tgtatatcat ttaccaatca atcgctccgc catcagtata
     1081 gccgccctgc cgctgaatgg ccggcccccc gggtagatga aggagtcagc tggaccgagc
     1141 tgggagcgct ccctccggtg ccggtggatc tgactaatga ttctataaaa aatattgtag
     1201 aacttggcaa agccctcttc tttgaccccc gtctcagcag ttcaggaaaa atttcctgtg
     1261 ccacctgtca tgatcctgcc ttaaactgga cggatgcaaa accccggtct gtcggacatg
     1321 aaggtgccat ggggaaaaga aatgccccaa cgatccagaa cagctggttt tataaaaaac
     1381 ttttttggga tgggcgttca cgtgacctgc aggatcaggc ttttgctccc atcgtcagtg
     1441 aatcagaaat gaatagtgag atgcacgatg tggtgcgcaa actacgccgc attaacgggt
     1501 atacggaatg gtttaaaaag gcctttggat cggaacatat taacccgcac ctgatgacgg
     1561 aagccattgc ggtatttgaa aaatcaatca gcagtggcga aagccgtttt gaccggttta
     1621 ttaaagggga tcaccgggct ttaaacaaaa gtgaattacg ggggcttcac ctgttccgca
     1681 ccaaggccgg ttgcttcaac tgtcataatg gccccctgtt taccgacaat cagtttcacc
     1741 ggctgggagc ggtgattaac aacccatatg atacaaaaga tgtgggatta tacaaagtca
     1801 cccacctgga tgccgatacc ggaaagttca gaaccccctc tctccgggat gtgatgcgga
     1861 ccggcccctg gtttcatgat ggctcagaaa ataatatgga cagccttatt ttccggtttc
     1921 atctcaacag cccgggcccc aatggtgtat tcagaccact gaacctcagc aggaaagaac
     1981 aaaaagacct ccgggccttc ctggaagcac tatcagctcc gccagttccc tttaatcgcc
     2041 cggctttacc gcaataggca actatttcaa ctgaaaacct atctttgttt atacacaaaa
     2101 atcaattcaa aaaagataca catgaaaaaa ttccttttaa cactttttgt tgctggtttt
     2161 ggttttatcg caaatgccca acctcctgag ggggaagcca aaccgggtga atggtatggc
     2221 gataaagtaa ccaccgaggg ggctgtaaat ctgaccgatg tggtagccaa actgaacggg
     2281 ggtgccgaat tcccggaagt aaagatccag gcgaaaattg tggaagtatg ccccaagaaa
     2341 ggctgctggc tcaaactcga actggcgaat ggtgaaacag cgatggttaa aatgaaagac
     2401 tatggtttct tcctgcccct ggcggctaaa ggaaaaacag taatcattga tggtaaagtg
     2461 gaactgaaaa ccacttccgt tgcagaactg aagcactatg ccgaagatgc caaaaaatca
     2521 aaagaagaaa ttgacgcaat cacccagccg gtgaaagaag tgaaagtgct ggcgaaaggg
     2581 attgtggtgg tgaagtaatc aacgacctta tttatacaag ggctgctccg gaaaggagca
     2641 gccctttttt tatgctttcc gttttgcgta aaccaatttc ccgatacggc aggtatacag
     2701 actcaatgca ttttagtttt gttcccgcaa gaataatgtg taaaacgaaa aatcattgaa
     2761 atgaaaacaa ccacactgct tatagctatt ctcttcttac ttgtttccgg catccgggcc
     2821 caggaaactg aaacgacact caacggaacc gttaaaacca gcgatggaaa gccggcctcc
     2881 atggtcagtg tcattctcaa agaattgaaa aaaggcgtga tcactgacga aattggtcaa
     2941 tttcagttta agggcattaa agaaggacgc tatacgatta ttgtatcctt cgtgggactc
     3001 gaaaccgtgg aatcacaagt gaactttaac gggaaggata accgcatatt tgatttcgta
     3061 ctccgggaaa accataacga actggaagaa atcgtgatta cctccgggta tacatccaat
     3121 gaaaagaaaa tgaccaccgg aaaaatcaat atacgcctga tggacctacc gcaagccgta
     3181 accgtgatca gtaaagaagc gattgagcgg caacaggccc tgcatctcag tgatgtgctg
     3241 cagcaggtca acggtgtgta cctgatggga aatacgggag gtttccagga agaaattgcc
     3301 gcccggggat tttcgttttc gagttcaaat acttttaaaa atggagtgcg gttcaataat
     3361 gcaattatgc cggaaatgag cagcatcgag aaactggaat ttttaaaggg gggtagtgcg
     3421 atcctgtttg ggaatgtggc tcccggtggc atcatgaata ttgtgactaa aaaacccaaa
     3481 tttgaacagg gtggggaact gagtttccgg accggaagtt atggattcta taaaccttcc
     3541 ctggatgtgt acagtggttt tggtaaaaag aaacaggccg cattccgggt aaatactgtt
     3601 tatgaaaagg cgcagagttt ccgggattat gtatcgtccg aacgttatta tattaatcca
     3661 tcccttttat ttaacctgtc ttcaaaaaca gaattgttaa tccaggggga tta
//
LOCUS       DAOPMW010000345         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29741_length_3703_cov_9.793860, whole genome shotgun sequence.
ACCESSION   DAOPMW010000345 DAOPMW010000000
VERSION     DAOPMW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29741_length_3703_cov_9.793860"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..983
                     /locus_tag="PLU11_13215"
     CDS             33..983
                     /locus_tag="PLU11_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HPN60138.1"
                     /translation="MKKIILLTGSCLLLLVISAQPVRQFGQLSVKGTQLISADGKPVV
                     LHGMSFGWHNLWPRFYNKGAVHELVKKWNCTVIRASMGIELNDSGYLKAPARSEQLMR
                     NVIDACIKENVYVIIDWHDHNLHEKEAIEFFSRMAADYGTNPHVIYEIFNEPDYETWP
                     EVKNYSEKVIAAIREKDPDNIILLGTPRWDQDIHLAAADPVKGYNNLMYTMHFYAGTH
                     KQWLRDRTDEAIKAGLPVFVSECAGMEATGDGPVDHKEWEAFVKWMDSRKLSWIAWSV
                     SDKKETCSVLLPAARSRGKWKGNVIKEWGQLVRGYLAAYR"
     gene            1098..1223
                     /locus_tag="PLU11_13220"
     CDS             1098..1223
                     /locus_tag="PLU11_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60139.1"
                     /translation="MKGRILIVLIIAVIVFESCGRVMTPYQAANNPRGKKCAVIR"
     gene            complement(1228..1374)
                     /locus_tag="PLU11_13225"
     CDS             complement(1228..1374)
                     /locus_tag="PLU11_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60140.1"
                     /translation="MQELVDKLKAEAGLTDEQAKKAISTIKNFVVEKFPMLEGAVNNV
                     FGGD"
     gene            complement(1495..2739)
                     /gene="clpX"
                     /locus_tag="PLU11_13230"
     CDS             complement(1495..2739)
                     /gene="clpX"
                     /locus_tag="PLU11_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPN60141.1"
                     /translation="MAKNPLHCSFCGRSRDEVKILIAGQEGHICENCVEHAREIIEQE
                     LTVKDDRSSSSFKLTVKKPIEIKKFLDEYAIGQDEAKKVLAVAVYNHYKRLQQKVSDL
                     NTGNEVEIEKSNIIMVGETGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVES
                     ILTRLLQVCNYDVTSAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGMLKLLEGT
                     DVLVPPQGGRKHPEQKLIKINTQNILFICGGAFDGIDKIIARRVQTNTIGFNVDKELQ
                     DNMKKNLLSYVNATDLKTFGLIPELLGRLPVVTHLDPLDAVTLRAILTEPKNALIKQY
                     SKLFELEGIELSIENDVLDFMVEKAMEFKLGARGLRSICEGILTDAMYELPSSNETQF
                     VLDLEYAKRKFDKSKLSLLKVA"
     gene            complement(2867..3496)
                     /locus_tag="PLU11_13235"
     CDS             complement(2867..3496)
                     /locus_tag="PLU11_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPN60142.1"
                     /translation="MNRNNYQYSNRRMNEDEENDEAPKSDLGMFNKKLERYFFEKRAV
                     YLWGVVDDKSAKDVVSKLLLLDADKPGEEIKFYINSPGGVVTSGMVMYDTMQMIKSPV
                     STICMGLAASMGSILLSGGVKGKRYIFPHGEVMIHQPSLGGHIQGVSTDLEIQAEQTR
                     RVKEIGATILAKNCGKTVEQIMKDFDRDYWMDARKAIEYGIADHLVESL"
     gene            complement(3521..>3703)
                     /locus_tag="PLU11_13240"
     CDS             complement(3521..>3703)
                     /locus_tag="PLU11_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPN60143.1"
                     /translation="NEIRKLKRELYEVVNNHTGKSLEQIEKDFDRDNWMTAIEAKEYG
                     LVDEVLLTNPRKQKKD"
BASE COUNT          943 a          871 c          808 g         1081 t
ORIGIN      
        1 tttgaaaagg acctgaagaa aatttataag cgatgaaaaa aattatcctg ttaaccggca
       61 gctgcctgct gctgctggtt atttccgccc aaccggtcag gcaatttgga cagttatctg
      121 taaaaggcac tcagcttata tcggctgatg gtaaaccggt ggttctccac ggcatgagtt
      181 ttggctggca taacctctgg ccccgttttt ataataaagg ggccgtgcat gaactggtaa
      241 agaaatggaa ttgcaccgtg atccgtgcct cgatgggcat tgaattaaat gacagcggtt
      301 atctcaaagc gccggcccgt tcggaacaac ttatgcggaa tgtaattgat gcctgcatca
      361 aagagaatgt ctatgtgatc attgactggc acgatcataa tctgcacgaa aaagaagcca
      421 ttgagttttt cagccggatg gccgcagact acggaactaa tccgcatgtg atctacgaaa
      481 tttttaatga acctgattat gagacctggc cggaagtaaa aaactattca gaaaaagtca
      541 tagcggctat ccgggaaaag gatccggata atatcatcct gctgggaaca ccccgctggg
      601 accaggatat tcatctggct gcagctgatc ctgttaaagg atacaacaac ctgatgtata
      661 ccatgcattt ttatgccggt acccataagc aatggctgcg ggatcgtaca gacgaagcca
      721 taaaagccgg tttgcccgta tttgtctcag agtgcgcggg tatggaagca accggagatg
      781 gtccggttga tcataaagaa tgggaggctt ttgtaaaatg gatggatagc cgcaaactca
      841 gctggattgc ctggtcggtc tcagataaaa aagaaacctg ttcagtacta ttgcctgctg
      901 caagatcccg tggaaaatgg aagggaaatg tgataaaaga atggggacaa ctggtcaggg
      961 gttacctggc tgcatacagg taagccggta tacgtagaac cccccggatt agggtaagat
     1021 attacggtca gcaaaacagg tttttgctat tgacccgcag caacaagtct cctacttttg
     1081 tccattaatt cgacactatg aaaggaagaa tcctgattgt actgatcatt gccgtaattg
     1141 tttttgaatc ctgtggccgc gtaatgactc cttaccaggc agccaataat ccgcgtggta
     1201 aaaaatgcgc cgttatccgt taatgtatta atctcctcca aatacattat tcaccgcgcc
     1261 ttccagcatc gggaacttct cgactacaaa atttttgatg gtggagatgg ctttctttgc
     1321 ctgttcgtct gtcagtccgg cttcggcttt cagtttgtct actaattcct gcatatagag
     1381 tttgttttag gaccgcaaaa taagaaaggt caggcgaatg cccgaccttt taaactttta
     1441 aactcttaaa ccttttaaac tttttaaacc tcttaaacct cttaaacctt tcctctacgc
     1501 cactttcagc agactcagct tactcttatc aaacttccgc tttgcatact ccagatccag
     1561 cacaaactgc gtttcgttgg aggagggtaa ttcatacatg gcatctgtca gaataccttc
     1621 acaaatgctg cggagtccac gggcgcctaa tttaaattcc atggcttttt ccaccataaa
     1681 atccagcaca tcattttcta tactcagttc aatgccttcg agttcaaaca gtttactgta
     1741 ctgtttaatg agggcatttt taggttctgt caggatagcc cggagtgtga ctgcatcaag
     1801 cggatccagg tgggtgacca cgggcagacg acccagtaat tccggaatca gtccgaatgt
     1861 tttcaggtcg gtggcgttta cgtaactcag caggtttttc ttcatattat cctgcagttc
     1921 cttatccacg ttgaaaccaa tggtattggt ttgtacccgg cgggcaatga ttttatcaat
     1981 gccatcaaaa gcgccaccac agatgaagag tatgttctgg gtattgattt tgatgagttt
     2041 ttgttcgggg tgtttacgcc ctccctgtgg tggaaccaat acgtccgttc cttcgaggag
     2101 tttcagcata ccctgctgca ccccttcccc gctgacatca cgggtgatag aggggttatc
     2161 gcctttacgg gcaattttgt caatctcgtc gatatataca ataccgcgtt cagcagaagt
     2221 gacgtcatag ttgcagacct ggagtaaacg ggtgagaatg ctttccacat cttcgcccac
     2281 atatccggct tctgtaaata ccgtggcatc cacaatggca aaaggcacat tcagcatacg
     2341 ggcaatactc ttggccagca gggttttacc ggtaccggtt tccccaacca tgatgatatt
     2401 gctcttctca atctccacct cgttgccggt gttgagatcg gatacttttt gctgtagacg
     2461 tttgtagtga ttatatacgg caacggccag tacttttttg gcctcatcct gaccgatggc
     2521 gtattcgtcc aggaatttct tgatctcaat gggtttctta accgtgagtt tgaaagaaga
     2581 gctcgagcgg tcatctttga cagtcagttc ctgttcgatg atttcgcggg catgctccac
     2641 acaattctca cagatatgcc cttcctgtcc ggcgatcagg atcttaactt catcgcggct
     2701 tctgccacaa aaagaacaat gtaacgggtt ttttgccatg ttgcttaacc ttaacttaac
     2761 cttaaacaca aatccggctt caggtaatat acaaaaatat aaaaaaccgc cggtaaaagg
     2821 cggtttttat ttacaaaaat aatgcaaagc acttatatat aataggttag agcgactcaa
     2881 ccaggtggtc tgcgatgcca tattcgatcg ctttccgggc atccatccaa tagtcgcggt
     2941 caaagtcctt catgatctgt tcaactgttt ttccacaatt tttggccaga atggttgctc
     3001 cgatctcttt aacccggcgg gtttgttcgg cctggatctc cagatccgta ctcactccct
     3061 gaatatgtcc gcccagggaa ggctggtgta tcatcacttc accatgtggg aaaatatagc
     3121 gcttcccttt aacaccacca ctcagtaata tactgcccat agaggccgcc aatcccatgc
     3181 aaatggtact gaccgggctt ttaatcatct gcatggtatc atacatgacc ataccgctgg
     3241 tgaccacacc acccggactg ttgatataga attttatctc ttcaccgggt ttgtctgcat
     3301 ccagcagcag gagtttgctg accacatctt tggcactttt atcatcaact acaccccata
     3361 ggtataccgc tcttttttca aagaaataac gctccagttt tttgttgaac attcccaggt
     3421 cgcttttagg ggcctcgtca ttttcttcgt cttcgttcat tctccggttg gagtattggt
     3481 aattattacg gttcattttt ctgtattaat aagtaatgaa ttagtctttt ttctgtttgc
     3541 ggggattggt cagcagtact tcatccacca gtccgtattc tttggcctcg atcgcggtca
     3601 tccagttgtc gcggtcaaaa tccttttcaa tttgttcgag tgatttaccc gtgtggttat
     3661 tcaccacttc ataaagctct cttttgagct tccggatctc att
//
LOCUS       DAOPMW010000346         3699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29773_length_3699_cov_8.214325, whole genome shotgun sequence.
ACCESSION   DAOPMW010000346 DAOPMW010000000
VERSION     DAOPMW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29773_length_3699_cov_8.214325"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLU11_13245"
     CDS             <1..378
                     /locus_tag="PLU11_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60144.1"
                     /translation="TSGQMAPTTLPGMHTTTSPDGRDVSYYGAPIKVSELMSNLPGSF
                     YVTRQAVNNPNAVRRTKKALLNAFKYQQVHKGTCLVEIIGNCPSNWKMTPVEASKFID
                     EEMIKTFPLGDIKIPNAEDLKIF"
     gene            397..978
                     /locus_tag="PLU11_13250"
     CDS             397..978
                     /locus_tag="PLU11_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPN60145.1"
                     /translation="MNSLNDCRQLEELIVAGFGGQGVLSLGMTLAYAGMVENKEISWM
                     PSYGPEMRGGTANCIVILSENKISSPILSQFDSAIVLNQPSMDKFASRIKPGGLLLYE
                     SGNIFKPATREDIDIIGIPATTEALKMKNAKTMNMIMLGAYLQLKPVVKNESILEALA
                     KVLPEKYHHLLPVNRQALELGEALARELKPVLV"
     gene            1055..1603
                     /locus_tag="PLU11_13255"
     CDS             1055..1603
                     /locus_tag="PLU11_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60146.1"
                     /translation="MTKFRGCFSTIVLCCIMAGIYGILIDQLTYTVSPEYYTQYKFPL
                     YHINPFEFGGDRMAAIVIGFIATWWTGLFIGAGIGFTGLIFENQRIRRKNMAIAVLIV
                     LITTLLLGVIGYFFGVMIEVDEVVENRRLIQSLANPVDFIVVGYVHQYSYVGAFLGLF
                     LGISFLFTQKAKSNFLQAKFAV"
     gene            1600..2244
                     /gene="pdxH"
                     /locus_tag="PLU11_13260"
     CDS             1600..2244
                     /gene="pdxH"
                     /locus_tag="PLU11_13260"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPN60147.1"
                     /translation="MTAEEIARLRKDYALNSLNEDEVADSPIIQFEKWWDDASKSEIL
                     EYNAMTLASTGHDGMPEARTVLLKAFDEKGFVFFTNYNSAKSQQLDFNANCCLLFFWK
                     ELERQVRVNGTAERITMKESIDYFDSRPDGSKIGAWASPQSMVVAGKAWLKETFEYYA
                     ERFKHGQIPKPPHWGGYRVKPTKIEFWQGRPSRMHDRILYTPEGEGWKRERLAP"
     gene            complement(2578..3417)
                     /locus_tag="PLU11_13265"
     CDS             complement(2578..3417)
                     /locus_tag="PLU11_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN60148.1"
                     /translation="MPIYLCSLNSGSNANCYYVGNADEAILVDTGLSCRETERRMQQR
                     ELDMGKVKAIFISHEHSDHITGMSGLSKKYHIPVYITNGTFLNSRMPLEEDLICTFKH
                     AAPVNIGGLTITPFKKSHDAADPHSFIITGNGVHIGVITDIGYACKKVIKYFGQCHAA
                     FLESNYCPDMLRNGSYPYHLQKRISGDEGHLSNHQALELFQQYRSPELQLLILSHLSK
                     NNNSPETVANLFAAHAAGVNVVVASRYEAGEVFCLEDKEVPKEVMKTRKKVLKNENQL
                     SLF"
BASE COUNT          945 a          826 c          911 g         1017 t
ORIGIN      
        1 accagtggcc agatggcacc caccacttta ccgggtatgc atactactac cagtccggat
       61 ggccgggacg tgagttatta tggggcaccg attaaagtat ccgaactgat gtccaacctg
      121 cccggttctt tttatgtcac ccggcaggcg gtaaataatc cgaatgctgt ccgcagaaca
      181 aagaaagcct tattaaatgc gtttaaatac cagcaagttc ataaaggcac ttgcctggtg
      241 gagatcatcg gtaattgtcc gtccaactgg aagatgaccc cggtggaagc ctctaagttt
      301 attgatgagg aaatgatcaa aaccttcccg ttgggggata ttaaaatccc caatgcggag
      361 gaccttaaaa tattttaatt aataaaacga accgatatga acagcctgaa tgattgccgc
      421 cagctggaag aactgattgt tgctggtttt ggtggccagg gtgtattatc acttggcatg
      481 acgctcgcct atgcggggat ggtggagaat aaggaaattt cctggatgcc ctcttatggt
      541 ccggagatgc ggggtggtac ggctaactgt attgtgatcc tgtcggaaaa caagatcagt
      601 tccccgatcc tgtcccagtt tgattccgcc attgtgctga atcagccatc catggataag
      661 tttgcctccc ggatcaaacc cggtggttta ctgttatatg aatccggaaa tatcttcaag
      721 ccggctaccc gcgaagatat tgacatcatt gggatacccg ccacaactga agcattgaag
      781 atgaagaatg ctaaaacgat gaacatgatc atgctcggtg cttacctgca gctgaagccg
      841 gttgttaaaa atgaatctat actggaagca ctggccaaag tgctgccgga gaaataccac
      901 catctcttac cagtcaatcg ccaggcactg gaactgggtg aagcattggc ccgggaactg
      961 aagccggtat tggtgtgacc gcatttctga ttagtaacag gtagccgggg taacccggct
     1021 tttttttgta tatttccctt gccaaacata ttctatgaca aaattccggg gctgtttcag
     1081 caccattgta ctttgttgca ttatggcggg tatctatggt atcctcatcg accagctgac
     1141 ctatacggtc tcaccggaat attacactca atataagttt cccctttatc atatcaatcc
     1201 gtttgagttt ggcggcgacc gcatggctgc gatcgtaatt gggtttatag ctacctggtg
     1261 gaccggcctt ttcataggag cggggattgg tttcaccggt ttaatctttg aaaatcagcg
     1321 tatacgcaga aagaatatgg ctattgccgt tctgattgta ctgataacca cattgttatt
     1381 gggtgtgatt ggctattttt ttggcgtgat gattgaagtg gatgaagtgg tggaaaacag
     1441 gaggctgatc caatccctgg ctaatccggt tgactttatc gttgtgggat atgttcacca
     1501 gtacagctat gtcggggcct ttctgggtct tttcctgggt attagttttc tctttacaca
     1561 aaaagcgaag tctaactttc tccaggctaa atttgcagta tgacagcaga agaaattgcc
     1621 cggctccgga aggattatgc attgaattca ctcaatgaag atgaagtggc cgactcgcca
     1681 atcatccagt ttgaaaaatg gtgggacgat gccagcaaat ctgagatact ggaatataat
     1741 gccatgaccc tggccagtac cggtcatgat gggatgcccg aggcccgcac cgttttactg
     1801 aaagcctttg atgaaaaagg atttgtcttt tttaccaatt acaatagtgc caagagtcag
     1861 caactggact tcaatgccaa ctgttgttta ttatttttct ggaaagaact ggaacgccag
     1921 gtacgtgtca atggcactgc tgagcggatc acgatgaaag agagcattga ttattttgac
     1981 agtcgccccg atggcagcaa aatcggtgcc tgggcctccc cccagagtat ggtagtggcg
     2041 ggtaaagcct ggctgaaaga aacctttgaa tactacgcgg aacgtttcaa acacggacag
     2101 attcccaagc ccccgcactg gggtggttac cgggtaaaac ccactaaaat agaattctgg
     2161 cagggaagac cgagccggat gcatgaccgc attttgtata cgccggaggg agaggggtgg
     2221 aagagggaaa ggttagcacc ttaggctagt cggtagttag tagttcgtag ttagtagcct
     2281 gcctgccggc aaaggcaggt caacagccca ttctgaggag atatgtctga aacaacaaac
     2341 tgatgctgct atttcgagcg taacgagaaa tcttgtattt ccctttttta tcccctacat
     2401 gcgttgtcat tccgacgaag gaggaacctt gtatttagaa tctggtgttc ggtgttagtt
     2461 gtttgtcaac agctcctcct tagaatgttt atcaagagca acaaacagtt actcatttta
     2521 agaaatctgc tcgcatctcg caactcgcat ctcgcaactc cttctgtcat gctctagcta
     2581 gaacagtgat aactggtttt cattcttcag caccttcttc ctcgtcttca ttacttcctt
     2641 tggtacttct ttatcctcca ggcagaagac ttcaccggct tcgtatctgg aagcgactac
     2701 tacattgaca cctgctgcat gtgctgcgaa gagattggcg actgtttcgg gggaattatt
     2761 gtttttggat aagtgggaga ggatcagcag ttggagttca ggggaacgat actgctggaa
     2821 taattccagt gcctggtggt tggacaggtg accttcgtcg ccgctgatgc gtttttgcaa
     2881 atgataagga taggatccgt tgcggagcat atccggacaa tagttggatt ccaggaaggc
     2941 ggcatggcac tgaccgaaat atttaatcac ttttttacag gcatacccga tatcggtgat
     3001 cacgcctata tgtactccgt taccagtaat aataaagcta tgtggatcag ccgcgtcatg
     3061 tgatttcttg aacggggtaa tggtcagtcc accaatgttg accggtgccg catgtttgaa
     3121 tgtgcagatc aggtcttctt ccaggggcat ccggctgttg aggaatgtac cattcgtgat
     3181 ataaaccggg atatgatact tcttcgacaa tcccgacatc ccggtgatat gatcactatg
     3241 ttcatgggaa atgaaaatcg ccttcacttt tcccatatcc agctcccgtt gctgcattct
     3301 ccgctcggtt tcccggcaac tcagcccggt atccaccagg atcgcttcat cagcgttacc
     3361 gacatagtaa caattggcat tactgcctga attaagggag cagaggtaaa tgggcatgag
     3421 gcgctaaagt acggaaaaaa gctagaggct agaagctaga agcgaaaagc gagaagcgag
     3481 aagcgagaag caagaagggg cctcctgagg gaataatcta ttcaattatg tgttttcctc
     3541 tttaggattc cttctcgctt ctcgcttcta gtttctagct tactttattg tcgcctctgt
     3601 tccttcagcc caatacttat actccggcgt ataatacaga taaaccgtgc tggcctttgc
     3661 tttatacgtt ccggggattt cggctttcag gtccagtcc
//
LOCUS       DAOPMW010000347         3680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_29981_length_3680_cov_4.070345, whole genome shotgun sequence.
ACCESSION   DAOPMW010000347 DAOPMW010000000
VERSION     DAOPMW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29981_length_3680_cov_4.070345"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PLU11_13270"
     CDS             <1..1161
                     /locus_tag="PLU11_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN60149.1"
                     /translation="VSGAAISWTALGGALNYDVDYKLASGSTWTNAATATTATSVNLS
                     GLTENSLYDWRVRANCTGATGTYSTAQFTTLSSTPTCGTVSGLTSSGITTAAATVSWT
                     TLSGALNYDVDYKTNASATWINAATGTTSTSVSLSGLAAGTLYDWRVRANCTGATGAY
                     TQAQFTTTAAVTCPGPYDVSTNGTASGAATIPFNTDVKGLINPSGDNDYYRFVITTGG
                     TATITLTTLPANYNIRLYSSNGTTLLASSTRNGTNTETISRTYTAGTYFVRVNGSNSN
                     AFNATNCYTLRVALGTASSPEGGIMEPVNEKPVLALFPNPVRQQLTLYVANYDKQKVV
                     EIYNISGQRVMRLNAMDNAMTLDVSKLKSGMYLLRVSSTEGRLISQEKFIHE"
     gene            1247..2014
                     /locus_tag="PLU11_13275"
     CDS             1247..2014
                     /locus_tag="PLU11_13275"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HPN60150.1"
                     /translation="MKYLYLLLIPFLVNSCQQPEASVYPQNKLNETEVHPSTIGHIRL
                     PDGFSRSPNPSGTFASWLRNLALKQDKTVYLYNRQLKKNQSAQYAVLEMPRSTTDLQQ
                     CADVLMRLRAEYLFSQQRYTDIRFTDFEGREYAWKNSSNRNEFERYLTNVFGWCGSAS
                     LEKQLTPVPDFNSIEPGDVLIRGGFPGHAVMVADMALNKNGEKIFTLVQGYQPAQDMH
                     ILLNPLAQDVSPWYSIPAGDTIYTPEWVFYKQQLRRW"
     gene            2114..3136
                     /locus_tag="PLU11_13280"
     CDS             2114..3136
                     /locus_tag="PLU11_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPN60151.1"
                     /translation="MLAYMDSLTHIAIGACIGEVFAGKKLGKKALLWGALAQSIPDID
                     FIATAWSSTSENLLAHRGFTHSFLFAILITPLLAFAADRWHRPHTITFKKWLFFFGTQ
                     ILIHLLIDGMNVYGAGWFEPFSHQRVSYNWIYVADPFFSIWPGISLLVLLLSNRNFRL
                     RQFWARSALLAGSVYLLYCGLNKAKIDTEVRRIMASQQVPYSRYFTTPTPLNNWLWYV
                     VAAADSGFYIGYRSVFDKKDSIRFHYVAQQQSLLKPVAGQEDLHNLLRFSNGYYSVAL
                     HNDTLLYNDIRFGEQIGWRSSDAPFVFYYYLEHPDANKLVVQRGRFAGWDRKAFRSLL
                     NRIRGE"
     gene            complement(3137..>3680)
                     /locus_tag="PLU11_13285"
     CDS             complement(3137..>3680)
                     /locus_tag="PLU11_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPN60152.1"
                     /translation="VQIWRSYLEAFSHSEKELTIMCSYFIPGLLYRRKLAQAVKKGVR
                     VRVILAGISDIKMAKHAERFLYDWMLKNKIAIFEYQETVLHAKVATCDGSWTTVGSYN
                     VNNISAYASLELNLDIRDPGFAGITTDMMETIIQDHCKEITPENYLNKVNFFTRVWQR
                     FSYGVFNTVLKLFTFYFRQE"
BASE COUNT          916 a          842 c          893 g         1029 t
ORIGIN      
        1 gtttccgggg ctgccatcag ctggaccgca ttgggtgggg cacttaatta tgatgtggat
       61 tataaactgg catcgggttc cacctggacc aatgctgcca cagctactac tgctacttcg
      121 gttaatctca gcggacttac ggagaacagc ctttatgact ggagggtgcg ggccaactgt
      181 accggtgcta ccggtaccta cagtacggct caattcacga ccctctcttc cacaccaacc
      241 tgtggaacgg tgagcggtct gacttcttcc ggcattacga cagctgccgc tacggtcagc
      301 tggacgactt taagtggcgc ccttaattat gatgtggatt ataaaacgaa tgcttcggct
      361 acctggatca atgccgctac cggaactacc tccacttcag ttagtctgag cggattggct
      421 gccggtactt tatatgactg gagagtccgc gccaattgta ccggagctac cggtgcttat
      481 acccaggcgc agtttaccac gacggctgct gttacctgtc ctggtcctta tgatgtgtca
      541 accaatggta cagccagtgg agcggccact attccattca ataccgatgt aaaagggctg
      601 attaatccga gtggtgataa tgattattat cgttttgtca ttacaacggg tggcacggca
      661 acgattacgc tgaccacttt gccagccaat tataatatcc gcttgtatag cagtaatgga
      721 acaacgctgc tggcttcttc aacccggaac ggaacgaata cggagacgat ttcgcgtacc
      781 tatacagcag gtacttattt tgtcagggta aacgggagta acagcaatgc ctttaatgct
      841 acgaactgtt ataccttaag ggtggcgctg ggtaccgctt cttctccgga aggcggaatc
      901 atggagccgg ttaatgaaaa accggtactg gccctgttcc cgaatccggt tcgtcagcaa
      961 ttgaccttgt atgtcgccaa ctacgataag cagaaagtgg tggagattta caatatcagc
     1021 ggacagcggg ttatgcggct caatgcgatg gataatgcga tgaccctgga tgtaagtaaa
     1081 ctgaaaagcg gcatgtatct gcttcgggtg agcagtacag aaggccggct gatcagccag
     1141 gagaaattta ttcacgagta aaataaaatc atgtcctcaa agccccgtgt tgcattacac
     1201 ggggcttttt tgttatggaa attcatcttc tgttcatctt ttagggatga aatacctgta
     1261 tctgctgcta ataccctttt tagttaattc ctgtcaacag ccggaggcat cggtttaccc
     1321 gcagaataag ttgaacgaaa cggaagtcca tccatcaaca atcggccata tccgcttgcc
     1381 ggacggattt tcccggagtc ccaatccgtc cggaacgttt gccagttggt taaggaatct
     1441 ggctttaaag caggataaga cggtgtattt atataacaga cagctgaaga agaatcagtc
     1501 tgcccagtat gcagttttgg agatgccccg tagtaccaca gaccttcagc aatgtgcgga
     1561 tgtgctcatg cggttaagag cggaatatct tttttcacaa caacgatata ctgacatccg
     1621 gttcactgat tttgaaggtc gtgaatatgc ctggaagaac agcagcaaca ggaacgaatt
     1681 tgagcggtac ctgacgaatg tgtttggctg gtgcggctcg gcatctttgg aaaagcaact
     1741 gacaccagtt ccggatttta attcaatcga accaggcgat gtattgatcc ggggaggttt
     1801 tcccgggcat gcagtaatgg tagccgacat ggcgttgaat aaaaatgggg agaaaatttt
     1861 tacactggtt cagggctatc agccggcaca ggatatgcat atattattga atccattagc
     1921 tcaggatgtt tctccctggt attctattcc cgccggtgat acaatttata ccccggaatg
     1981 ggtattttat aagcagcagt tacgacgctg gtgactttat ttgttttagc gggttctgtt
     2041 agatatcgta aagaaaataa atagtcctga atggaagaaa atcatacaat cctgcctgga
     2101 cttttaatag attgtgttag catatatgga ttccctgacg catatcgcca taggggcttg
     2161 tatcggagag gtcttcgccg gaaaaaagct gggaaagaaa gccctattat ggggcgcact
     2221 ggcccagagt ataccggata ttgattttat tgccacggcc tggagcagta catccgaaaa
     2281 tttgctggca caccggggct ttacccattc ttttcttttt gctatcctga tcactccttt
     2341 gttagctttt gcggctgacc gctggcaccg cccgcatact attacattta agaaatggct
     2401 gttttttttc ggcacccaga tcctgatcca tttactgatt gacgggatga atgtgtatgg
     2461 cgccggatgg tttgagccgt tcagtcatca acgggtttct tataactgga tttatgtggc
     2521 ggatccgttc ttctctatct ggccagggat cagtttattg gtcctgcttt tgtccaaccg
     2581 aaacttccgt ttacgtcaat tctgggcccg gtcggcattg ctggcaggat cagtatacct
     2641 gttgtattgc ggactgaata aagcaaagat cgacacggag gtcagaagaa taatggccag
     2701 tcagcaggtt ccttattccc ggtatttcac cacgcccacg ccgttgaata actggctctg
     2761 gtatgtggtg gctgctgctg attccggatt ttatatcggg taccgttctg tatttgataa
     2821 aaaagacagt atccgttttc attatgtggc acagcaacaa tcattgttaa aaccggttgc
     2881 aggacaggaa gacctgcata acctgctccg cttttcaaat ggctattatt ccgtggcact
     2941 acacaatgac acacttttgt acaatgacat ccgttttggc gaacaaatcg gctggcgaag
     3001 ctcggatgcc ccttttgttt tttattatta cctggaacat cccgatgcga ataaactggt
     3061 ggtgcagcgg ggtcgttttg ccggatggga ccggaaagcc tttcgttctt tgcttaaccg
     3121 gatcaggggg gagtaattat tcctgccgga agtagaaagt aaataattta agcaccgtgt
     3181 taaatacacc atagctgaac cgttgccata cccgggtgaa aaaattcact ttgttcagat
     3241 aattttccgg ggtgatttcc ttacaatggt cctgtatgat cgtttccatc atgtcagtcg
     3301 tgattccggc aaatcccgga tcccggatat ccagattcaa ttcaaggctg gcataggcac
     3361 tgatattgtt tacattatac gatcctaccg ttgtccagct accatcacag gtggccactt
     3421 ttgcatgcaa cactgtttcc tggtattcaa agatggcaat tttatttttc agcatccagt
     3481 catatagaaa ccgctccgca tgtttagcca tttttatatc ggatatgccg gccagaatca
     3541 ctcttacccg gacgcctttt ttaactgcct gggccagttt gcgccggtag agcaagcccg
     3601 gtataaaata actgcacatg atcgtcagtt ctttttctga gtggctgaac gcttccaggt
     3661 aggaacgcca gatctgcact
//
LOCUS       DAOPMW010000348         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30029_length_3675_cov_4.455525, whole genome shotgun sequence.
ACCESSION   DAOPMW010000348 DAOPMW010000000
VERSION     DAOPMW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30029_length_3675_cov_4.455525"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..763
                     /locus_tag="PLU11_13290"
     CDS             296..763
                     /locus_tag="PLU11_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPN60153.1"
                     /translation="MALDKTDIRLLELLQADARLTTKELADKLGKSVTPVYERKKRLE
                     QEGYITRYTAIVDKHKIGKSLVAFTNVQLKQHAQSILLNFEKVIVKFEEVMECYHMTG
                     VYDYLLKVAVTDMAAYQDFIVNKLAKLTDIGTVQSSFIMTEVKHETAYKIKMS"
     gene            complement(829..2253)
                     /locus_tag="PLU11_13295"
     CDS             complement(829..2253)
                     /locus_tag="PLU11_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN60154.1"
                     /translation="MKIEQIYTGCLAEAAYYIESNGEAAIIDPLRETKPYVDKADRNG
                     ATIKYILETHFHADFVSGHLDLANKTGATIVFGPTAAPAYDVHVATDGELLKLGNVTI
                     KVLHTPGHTMESTTYLLLDEKGKEYCIFTGDTLFIGDVGRPDLVQKVKAEITPEILGG
                     HLYDSLRNKIMPLPDEVIVYPGHGAGSACGKNMSKETTDTLGHQKDTNYALRADMTKE
                     EFIKAVTDGLVEPPQYFPFNVLLNLKGGMDSIDDIIRKGKNPLSAKEFEAIWESTDAL
                     VLDTRKKEEFAAEHIPGSIFIGVDDSFAPWVGTLITDLKQPILFVADEGREEEVIVRL
                     ARVGYDHTIGFLKGGVAAWKEAGYATELIKEISPEDFATLYEMDNKHTHLLDARRKSE
                     FDSEHLVGAENFPLDFINQNMAQLDRDKKYFIHCAGGYRSLIMCSILKSRGFDHLVNI
                     KGGYKVLSQTGLPKTKYTAPKTML"
     gene            2745..2954
                     /locus_tag="PLU11_13300"
     CDS             2745..2954
                     /locus_tag="PLU11_13300"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HPN60155.1"
                     /translation="MKNWVLNNKWTIAGVVLGAVAGYLYWQQIGCSSGTCMITSKWHN
                     STAYGALMGGLLFSMFKKEKHADKQ"
     gene            2938..3261
                     /gene="trxA"
                     /locus_tag="PLU11_13305"
     CDS             2938..3261
                     /gene="trxA"
                     /locus_tag="PLU11_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPN60156.1"
                     /translation="MQTSNKKESFGELIRGNTPVLVDFTATWCGPCKMMKPVLEQLHQ
                     RMGDKVRIIKIDIDQSPAAAQAYNVQSVPTLLLFQQGKQLWRQSGVIQATQLEKIIEQ
                     HTTNE"
     gene            3264..>3675
                     /locus_tag="PLU11_13310"
     CDS             3264..>3675
                     /locus_tag="PLU11_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HPN60157.1"
                     /translation="MSDKKENRGYLASALTCHCPRCREGKLFKHPLSLSFKKNLEMYK
                     SCPVCGQMTEIEVGFYYGTSYVSYAMTVALSVATLVAWWVIIGLSTEDNRFFYWMGFN
                     AAFLIILQPWLMRLSRSLWISWFVKFDPYWKENQP"
BASE COUNT          997 a          824 c          833 g         1021 t
ORIGIN      
        1 aagccttccc tgcttcagca gatttaaaaa caaaagtgga ggtctgaaaa atggggcatt
       61 taacagcacc ctcactcagt tcaggtttgt aaccataacc cagcatctgg gtttcggggg
      121 caaatgaatc ttgtttcata acggatcatt taggttgagt ccgaatttca tacaaaatac
      181 cagtcgaatg aatgatttta aaaaaagcag aaaacaaatc ttttttatcc tgatttacac
      241 tccgaaaaag atattttatc tgaattatta actaaattta gataaatcaa cttttatggc
      301 tttagataaa acagatatcc gcctgcttga attattgcag gcagacgccc ggctcaccac
      361 aaaggaactg gctgacaaac tgggcaaatc ggttaccccg gtttatgaac gaaaaaaaag
      421 gctggagcag gaagggtata ttacccgcta tacggccatc gtggataagc ataagatcgg
      481 caagagcctt gtggctttca ctaatgtgca gttaaaacaa catgcacaga gcatcctgct
      541 gaattttgaa aaagtgatcg tcaaatttga agaagtgatg gagtgttatc atatgaccgg
      601 cgtgtatgat tacctcctga aagtagccgt aacggatatg gccgcttacc aggattttat
      661 cgtcaacaaa ctggccaaac tcaccgatat cggtacggta cagagcagtt ttatcatgac
      721 cgaagtgaaa cacgaaacgg catataaaat aaaaatgtcc tgaaaaaaag atgctgcttc
      781 atcatttgaa acagcaccca atttgcagca agcaatcggt caaaaaaatc aaagcatcgt
      841 tttaggtgcc gtatattttg tttttggcag tccggtctgg ctaagcactt tataccctcc
      901 tttgatattc accaggtgat caaagccccg ggatttcaga atggagcaca tgatcaggga
      961 gcgatatccg ccggcacaat ggataaagta ctttttatcg cggtccagct gggccatatt
     1021 ctggttgata aaatctaatg ggaagttctc ggctcctacc aggtgctcag aatcaaactc
     1081 acttttccgt cgggcatcga gcaggtgggt atgcttattg tccatttcat ataatgtggc
     1141 aaagtcttcc ggactaatct ccttgatcag ttcagttgca taccctgctt ctttccaggc
     1201 agccacccct cctttcagaa aaccaatcgt atgatcatat cccacacggg cgagtcgtac
     1261 aatcacttcc tcctcacgtc cttcatcggc tacaaaaagg ataggctgtt taaggtcagt
     1321 gatcagggtg cctacccagg gggcaaaact gtcatccacc ccgataaaga tggagccggg
     1381 aatatgttct gcggcaaatt cttctttctt acgggtatcg agcacaaggg catcggtact
     1441 ttcccagatg gcttcaaact ctttggcaga aagtggattt ttccccttcc tgattatatc
     1501 atcaatactg tccatccctc ctttcaggtt cagtaataca ttaaatggaa aatattgcgg
     1561 cggttccacc agtccatccg ttacggcttt tataaactct tccttagtca tatccgcccg
     1621 gagcgcatag ttggtatctt tctgatggcc gagtgtatcc gtggtttcct tactcatgtt
     1681 cttcccgcag gcactgccgg caccatgacc tggatacaca atcacttcat cgggcagagg
     1741 catgatctta ttgcgcagtg aatcatacaa atgacctccc agaatttcgg gagtaatttc
     1801 cgcttttact ttttgtacca ggtccggacg acccacatct ccgataaaca atgtgtcgcc
     1861 ggtaaagatg caatactcct tccctttttc atccagcaag aggtatgtgg tggactccat
     1921 cgtatggccg ggcgtatgca gtactttaat tgtaacgtta cccagtttga gtaattcccc
     1981 atcagtagct acatgtacat cataagccgg ggcagcggtt ggaccgaaca cgatggtggc
     2041 ccctgtttta ttggccaggt ccaggtgtcc acttacaaaa tctgcatgaa aatgggtttc
     2101 aagaatatac tttatcgtag ccccgttccg gtcagctttg tctacatagg gtttagtttc
     2161 ccggagagga tcaattatgg cggcttcgcc attgctttca atataatacg cggcttctgc
     2221 gaggcagccg gtatagattt gttcaatttt cattgcgcat ttatttaatg gatggtgtat
     2281 atgttcatta catgaaactg ccaggagtaa atttgggtat tactcccggt agtttatgtg
     2341 cttctggtca cccaaagtaa atcccacgtg acgaaaccat gactgaccgt cgtcagtccg
     2401 gtacctgctt tcgctctgtt tgctgaaaac aggatatccg ggtttttttt gtaactatag
     2461 tcaccccttt cccggttccg attcaggaaa tttgcagaaa tgactgaaca tgaacatatt
     2521 tgaccggatt aaaagcgggt ggaccaccct gaaattcatt cgggttgggc tgggttcgtt
     2581 aattttgtat tcttccatca gtgaaggtca ggtgcctggt atatttcttg gcgggttttt
     2641 cctggtcttt tcattagtaa ccgatggcgt ttgttgtgca ggaaatgcct gttatacacc
     2701 gccagccaga aatttgaatc aaaaaacagt tgaaaataca gactatgaag aactgggtgc
     2761 taaataacaa gtggaccatc gccggggtag ttttgggagc cgtggcgggt tacctttact
     2821 ggcagcagat tggctgcagc agcggcacct gtatgattac gtccaaatgg cataacagta
     2881 ccgcctacgg agccctgatg ggcggattat tgttcagtat gtttaaaaaa gaaaaacatg
     2941 cagacaagca ataaaaaaga aagttttggg gaactgatcc ggggaaacac cccagtattg
     3001 gtggatttca cggccacctg gtgtggtccc tgtaagatga tgaaacccgt attggagcaa
     3061 ttacatcagc ggatgggcga taaagtgcgg atcattaaaa ttgatataga tcagagtccc
     3121 gctgcggcac aggcttacaa tgtacaaagt gtacctaccc tgttattatt tcagcagggc
     3181 aagcagttat ggcggcaatc cggggttatt caggcgacgc agctggagaa gatcattgaa
     3241 caacacacaa ccaacgaata agtatgtcag acaaaaaaga aaaccgtggc tacctcgctt
     3301 ctgccttaac ctgtcattgt ccccgttgcc gagaaggaaa attattcaag catccgttat
     3361 ccctcagttt caaaaagaac ctcgaaatgt ataaaagttg cccggtttgt ggtcagatga
     3421 ccgaaatcga ggtcggtttc tattatggca ccagttatgt aagttatgcc atgacagttg
     3481 ccctgagtgt ggccacattg gtcgcctggt gggtcattat cggtttgtct accgaggata
     3541 accgtttttt ctattggatg ggctttaatg ccgccttcct gatcatcctc cagccctggc
     3601 tgatgcgcct gagccggagt ctctggatct cctggtttgt gaaatttgac ccttattgga
     3661 aggaaaatca gcctg
//
LOCUS       DAOPMW010000349         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30165_length_3663_cov_6.155488, whole genome shotgun sequence.
ACCESSION   DAOPMW010000349 DAOPMW010000000
VERSION     DAOPMW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30165_length_3663_cov_6.155488"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1743)
                     /locus_tag="PLU11_13315"
     CDS             complement(85..1743)
                     /locus_tag="PLU11_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN60158.1"
                     /translation="MKAHQPFLLGLLIFIQLTSTAQTTWYQSQDGQLQPNGTYAAGIL
                     SLTRQSVVAAYMWRLDNENYTWKISKSHTNGTEQRTFFITAPYGIVEMKTGYRNSLYL
                     LVRSFPFAQDPQYTIYKLDSNLVVKAQRMISFAGSYTILNLNVFELDEAGNLYFAGDG
                     QYPDGPGFSPASFIMKTDKNMVTRWTRMDSVQTSYSRLHIERNGTIRLIEDFYTFFPD
                     IKVQKISAAGQLLQTRVYQTDPGRQSLTTLLDRDDNLLIAGTLDRTAGQSLYLHKIAR
                     HSGQVVYRKTFFNEVAANLDDVKLDEEGQLYALMTQYFNSSSQCRLARIQPRSGSLSW
                     SRTFPFSQDSCMLRNIVLSDDDRLYVIGDRRSGAYYTKGFAVQVKRNGQRGDTYIAPD
                     SVTFAISHTLFQGIIDQQNRLIVVGNTNDLDTVTGSSTYFRSFAMGMGRRHRDNDCED
                     DHHSNSLSESLMAKGAIAETAPLNISIYPNPATDRITVQNPDPEKYNQALIYDIRGNQ
                     LSLQYFNSHSASFDLSRMSAGFYLVVLRSSKGAADQTRTFTISR"
     gene            complement(1928..3601)
                     /gene="recN"
                     /locus_tag="PLU11_13320"
     CDS             complement(1928..3601)
                     /gene="recN"
                     /locus_tag="PLU11_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPN60159.1"
                     /translation="MLQRLSISNYAIIDELEIDFSGKLNVITGETGAGKSIIMGALGL
                     ILGERADTAALVKKEKKCVVEGFFQAGQKKAVAAFLKANELDNEDQLVLRREISTSGK
                     SRAFINDTPVNLSQLQQLSTLLVDLHQQFDTLTLGESGFQREVIDALASQTELLDEYQ
                     QAFRKWQVVKKEAEELKGQKQQFDKEADYNRFQFTELEEAGFKENELEDMDAELKLLN
                     NAEGIKAALSKTFFELSESESPMVQQLKIIINQLNPYTSYHPDLPSLLQRLQSAQIEL
                     QDIAGEVDRISDHVNYDPEKVERLNERLSLGYKLQKKHGVNTTAELLSIQAELQDKLQ
                     AVLNIDQAIEQKEKEKQQLYDKAALLAQKISANRKKQVKPFEDKVNKLLQQVGMPNAR
                     IKTMINPVELTESGADVVEFLFDANVPAGDQAKGQFLPLRKVASGGELSRLMLCIKSL
                     VAQSVDLPTMIFDEIDTGISGEAARQVGIIMKELAAKRQVICITHQPQIAGKADAHYF
                     VYKEIVKDLVKTNIRQLTREERITSIAKMLSGEKPTAAAMENAREMLMN"
BASE COUNT          906 a          872 c          837 g         1048 t
ORIGIN      
        1 aggaggggct gttgactacc gactaccgac tacgaactac taactagttt agggggctgt
       61 tgactaccgg cttccgacta cgaactaccg actaatagta aacgtccttg tctgatcagc
      121 ggctcctttg gaggaacgaa ggactaccag atagaaaccg gcactcattc gtgaaagatc
      181 gaaggaagct gaatggctat tgaaatattg tagggataat tgatttccgc ggatatcata
      241 aatcaaggcc tggttatatt tttccggatc cggattttgt accgtgatac ggtcagtggc
      301 cggattggga tagatcgaaa tatttaaagg ggctgtttca gctattgccc ctttagccat
      361 caatgattca gacaggctat tggaatgatg atcatcttcg caatcattat cccggtgacg
      421 acgccccatg cccatggcga atgaacggaa ataagtgctg cttccggtta ccgtatcaag
      481 atcattggta ttacctacca caatcagccg gttctgctga tcgatgatgc cctgaaacaa
      541 tgtatgcgaa atggcaaatg tgacactgtc cggcgcgata taggtatctc ctctttgccc
      601 gtttcttttc acctgtacag caaatccttt tgtataatac gcaccacttc tccggtcccc
      661 tataacatat aagcggtcat catccgatag tacaatgttc cggagcatac aactgtcctg
      721 cgaaaacgga aaagtccggc tccaggaaag agatccgctg cggggttgta tccgggccag
      781 ccggcattga ctgctgctat taaaatattg cgtcatcaag gcatacaatt gaccttcttc
      841 atccagtttc acatcatcca ggtttgcggc cacctcgttg aaaaatgtct tccggtacac
      901 gacctgcccg ctatgcctgg ctattttatg caggtataaa gactgaccgg ctgttctgtc
      961 caatgtacca gcgatcagca ggttgtcatc ccggtcaagt aaagtggtca gactctgacg
     1021 acctggatct gtctggtaca cccttgtttg tagtaattgg ccggccgcac tgatcttttg
     1081 tacttttata tccgggaaga aagtataaaa atcttcgatc agtcttattg ttccattccg
     1141 ttcaatatgc aaacgggaat aggacgtctg taccgaatcc atccgggtcc agcgggtcac
     1201 catgttctta tctgttttca ttataaaaga agccggactg aacccgggcc catcaggata
     1261 ctgtccatct ccggcgaagt aaaggttacc ggcttcatcc aattcaaaaa cgttcaggtt
     1321 taaaatggta tagctgcctg caaaactaat catgcgttgt gcctttacca cgagattgct
     1381 atccagttta taaatagtgt actgtggatc ctgggcaaaa ggaaaactgc gcacaagcag
     1441 atagaggctg ttccggtaac cggttttcat ttccacaatc ccatagggtg ccgtaataaa
     1501 aaaagtacgt tgctcggtcc cattcgtatg cgacttggaa attttccagg tatagttttc
     1561 attgtccagc cgccacatat aggccgctac aactgattgc cgggtcaacg ataaaatacc
     1621 ggcggcgtaa gtgccattcg gctggagttg tccatcctgc gactgatacc aggttgtctg
     1681 tgcagtactg gttaactgaa taaatattaa caggccaaga aggaagggct gatgtgcttt
     1741 catacatacg gattttctag ttcggaaggg acacctaaat gtatacaaga atcatgcacc
     1801 agaagcctga cccggagttg acctatgaat agttttttaa aaaaagggat gatacggcgt
     1861 agaagaagaa aacaggccgg ggttattccg tgaaaaaaca acagggataa atgagaaagg
     1921 aatgtgctta gttcatcagc atttcacgcg cattctccat agcggccgcc gtcggttttt
     1981 caccactgag cattttggca atactggtga tccgctcttc cctggtcagc tgcctgatat
     2041 tggtttttac gaggtctttg acaatctctt tataaacgaa gtaatgcgca tccgctttac
     2101 cggcaatctg cggctgatgc gtgatacaga tgacctggcg tttggcagcg agctctttca
     2161 ttataattcc tacttgcctg gctgcttcac cggaaatacc cgtatcaatt tcatcaaaga
     2221 tcatcgtggg cagatccacc gattgcgcca ccagtgattt aatgcaaagc atcaggcgac
     2281 tgagttcacc accacttgct acttttcgca gtggcaggaa ttgaccttta gcctgatctc
     2341 cagccggcac attcgcatca aataaaaact ccaccacatc ggccccggac tctgtcagtt
     2401 caaccggatt gatcattgtt tttatccggg cattgggcat gcctacctgt tgcaagagtt
     2461 tattgacctt atcttcaaaa ggcttcacct gttttttccg gttggccgat atcttttgtg
     2521 ccagcaaggc tgctttatca tacaactgct gcttttcttt ttccttctgc tctattgcct
     2581 gatcaatatt caataccgcc tgcagtttat cctgcagttc ggcttgtatg gacaaaagtt
     2641 cggctgtagt attcactcca tgtttcttct ggagtttata tccaagcgaa agcctttcat
     2701 tcaggcgttc taccttttcc ggatcataat tcacatgatc actgatgcgg tccacttccc
     2761 cggcaatatc ctgtaattcg atctgggcag attgcaaacg ttgaagcagg gaaggaagat
     2821 cagggtgata gctggtataa gggttcagct ggttgatgat gatcttcagt tgctgcacca
     2881 ttggactttc actttcactc aactcaaaga atgttttact caaagccgct ttgatgcctt
     2941 ccgcattgtt caggagtttc agttcggcat ccatatcctc cagttcattt tctttaaacc
     3001 cggcttcttc cagttcggta aactggaagc ggttataatc ggcttcttta tcgaattgct
     3061 gtttttgccc tttcagttct tcggcttctt ttttcaccac ctgccatttc cggaaagcct
     3121 gctgatattc gtccagtagt tcagtttggc tggcgagtgc atctattact tcccgttgaa
     3181 aaccggattc gcccaaggtc agggtatcga actgctgatg caggtctacg agtaaggtgc
     3241 ttaattgctg aagctgggaa agattaaccg gggtatcgtt gataaatgcg cgggatttac
     3301 cgctggtact gatttcgcga cgaaggacca gctggtcttc attgtccagt tcattggctt
     3361 tgagaaaagc ggccacggct tttttctgtc cggcctggaa gaaaccttcc accacacatt
     3421 tcttttcttt cttcactaaa gcggcggtat cggcgcgttc gccaaggatc agtccgaggg
     3481 cgcccatgat aatggattta ccggcaccgg tttctccggt gatcacgttg agtttgccgg
     3541 aaaaatcgat ttccagttcg tcgatgatgg cgtaattcga tatggagagt cgttgcaaca
     3601 ttgccgcagc aaattaagtt ttttcgacac aagttgggcc accgtgaaaa aggatttgcc
     3661 tgc
//
LOCUS       DAOPMW010000350         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30237_length_3656_cov_8.286587, whole genome shotgun sequence.
ACCESSION   DAOPMW010000350 DAOPMW010000000
VERSION     DAOPMW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30237_length_3656_cov_8.286587"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLU11_13325"
     CDS             <1..300
                     /locus_tag="PLU11_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HPN60160.1"
                     /translation="VGKDVPPYIKAGRTPLSYAGVNSIGLKRRGFNVERINHILDIYR
                     VLYNKGMNTSQAIEYLEEEMPATDERDEIVTFIRESGRGIIKRFTKGSNDDDLPI"
     gene            278..907
                     /locus_tag="PLU11_13330"
     CDS             278..907
                     /locus_tag="PLU11_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN60161.1"
                     /translation="MMTISLSEAGKRFNRDWIYRHFTYQFTSGNAYAITGPNGSGKST
                     LLQALSGSMLINEGSIDWHLDGKKLDPEMIYSHVSICAPYLELIEEMTLQEFLAFHQG
                     FKPFLPGLSANDIIARIGLEKAIHKQIRNYSSGMKQRVKLAQCILSDTSIVLLDEPCT
                     NLDAAGISLYLSLISDYGKDRLIVVSSNDETEYSFCGELVNISDYKLTK"
     gene            1013..1459
                     /locus_tag="PLU11_13335"
     CDS             1013..1459
                     /locus_tag="PLU11_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60162.1"
                     /translation="MSFIISVPDIHQFLYSPGLPIFAPVKKGIAFILLLIFTLQTFYT
                     SGFTVWFYANRKAIASEHCINKNRPELKCDGKCFLSMKMKEAGQHQDENAPLQVKQLL
                     EGSPYTLLNNYVELGMPESRIKHQSSYTIRYSYLHSNSLFRPPLSA"
     gene            1551..2378
                     /locus_tag="PLU11_13340"
     CDS             1551..2378
                     /locus_tag="PLU11_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HPN60163.1"
                     /translation="MKKILYLLLSFAVLCIIVFLSACSKDKTAQNPTAGLTKISEGYA
                     IGAGAKVEVYTKETSLTTGYTKFYLALYDSITGKRLDDAHIHLTPMMDMGMMQHSAPY
                     ENPASTDAVNYLFPCSVTFIMSSMGGSWTLEIEVHNHANDKEGSITLPITVAEPATSK
                     QKSFTATHNGAKYFISLISPTSANSKVGINDLEIAIYKKASMMSWPADSSLTVAVTPE
                     MPTMGHGSPNNVNPTHVANGHYKGKVNFTMTGLWRLNLDFMSGSNVADTTQYFDVEF"
     gene            2431..>3656
                     /locus_tag="PLU11_13345"
     CDS             2431..>3656
                     /locus_tag="PLU11_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN60164.1"
                     /translation="MKSFFFLLLLGSYLPGLAQNNNNSLSADSTRSILLSEVLVSTKT
                     RSDQQRMLQFYRSNQSSGLEDILSRLPEMSLIRRGTYGMEPAIRSLSGGQINVLVDGM
                     RIHGACTDKMDPATIYIEPINLEQLQLQTGTNGFINGSAIGGTLNLKTATPSCHTPNR
                     FSGTISTGFQSAAKNFYESMQLNYGTGKWSLRGTATWRKSSDYRSGGGERIAFSQYEK
                     VNYSFSAKYQLNQYNSIKADYIGDNGWNIGYPALLMDVGYAVARIGSVSLQQENPGQR
                     LYQWSAKLYANQVRHYMDDTHRPNVPMHMDMPGTSLTYGVFTEGMAKLNNKQQLRVVA
                     DFSATRLKASMTMYEPGQRPMYMLTWPDNRKDQAGIGVNWEYRADSNWLLQSVARLDF
                     ISTALTSQEAKDHISIL"
BASE COUNT         1025 a          886 c          797 g          948 t
ORIGIN      
        1 gtgggtaaag atgtacctcc ttatatcaaa gccggccgta caccacttag ttatgccggt
       61 gtaaactcca tcgggctgaa acgccggggg ttcaatgttg aacggatcaa tcatatcctc
      121 gatatctacc gcgtattata taataagggc atgaatacca gccaggctat tgagtacctg
      181 gaagaagaaa tgccggctac cgatgaacgc gatgagatcg ttacttttat ccgggaaagc
      241 ggccggggca ttatcaaacg cttcaccaaa ggcagcaatg atgacgatct ccctatctga
      301 ggccggaaag cgtttcaacc gtgactggat ctaccgtcat ttcacctacc agtttacttc
      361 aggcaatgcc tatgccatca ccggtcctaa cggatccggg aaaagcaccc ttttacaagc
      421 gttaagcgga tcgatgctta ttaacgaagg aagtattgac tggcacctgg acggaaaaaa
      481 gctcgatccg gaaatgatct atagccatgt ctccatctgt gcaccttatc ttgaactcat
      541 tgaggaaatg accctgcagg agttccttgc ttttcaccag ggtttcaaac cttttctacc
      601 cggactgagt gccaatgata tcattgcacg gatcggattg gagaaagcca ttcataaaca
      661 aatcaggaat tattcctcgg gcatgaaaca acgggttaaa ctcgctcaat gcattctctc
      721 agatacctcc atcgtattac tggatgaacc ctgcaccaac ctcgatgcag caggtatttc
      781 actttacctt tcgctgatca gcgattatgg caaggaccgg cttattgtgg tgagtagtaa
      841 tgatgagacg gaatactcgt tctgtgggga gctagttaat ataagtgatt ataagttgac
      901 taagtaagta gttgacaggt tgataagtta acaagttgat aagggggctt cttagtacac
      961 gtccttgtca acttgttaac ttattaactt ttcaacccta aaaaagaccc ctatgtcttt
     1021 tatcatctcc gttcctgata ttcatcaatt cctctattcc cctggcctcc ctatatttgc
     1081 acctgtgaaa aaagggatcg ctttcatatt actactgatc ttcaccctgc aaaccttcta
     1141 tacatcgggt ttcacagtat ggttttatgc caaccggaaa gccatcgcca gcgagcattg
     1201 tatcaataaa aaccggccgg aactgaaatg tgatggaaaa tgttttctca gcatgaaaat
     1261 gaaggaagcg ggtcagcacc aggatgaaaa cgccccgctc caggtaaaac aactgctgga
     1321 aggaagccct tatacccttt tgaataatta cgtagaactg ggaatgcctg aatcaaggat
     1381 aaaacaccag tcatcctata ccatccggta cagctatctc cacagcaatt cccttttccg
     1441 tccgccgctg tctgcctgat acatacgccg ttcatttatt gcatgctgat cctgttgccc
     1501 cgcagcagtt atccctctat actattcctt aacttaatca atcttgaatt atgaagaaaa
     1561 tactatacct gctattatcc tttgcggtcc tctgtatcat cgttttccta tccgcctgtt
     1621 caaaagataa aacagcacaa aatcctaccg ccggactgac caagatctct gaaggctacg
     1681 ccataggtgc cggtgctaaa gttgaagtat acacgaaaga gacttcgctt accacgggat
     1741 atactaaatt ttacctggcc ctttatgact ccattaccgg caaacggctg gatgatgcgc
     1801 atattcattt gactccgatg atggatatgg ggatgatgca acactccgcc ccctatgaaa
     1861 atccggcttc taccgatgca gttaattacc tgttcccctg cagtgtcact tttattatgt
     1921 cctcgatggg cggcagctgg acactggaaa ttgaagtgca caatcatgcc aatgacaaag
     1981 aaggttccat tacccttcca attaccgttg cggaaccggc cacttcaaaa caaaagtcat
     2041 ttacagccac acataatggg gcgaaatact ttatttccct gatcagtcct acgtctgcca
     2101 attcaaaagt gggtatcaat gatctggaaa tcgccatata taaaaaggca tccatgatga
     2161 gctggcctgc agacagcagt ctcaccgttg ctgttacacc cgaaatgccc acgatgggtc
     2221 atggctcacc caataatgta aatccgacgc atgtggccaa tggccattat aaaggcaagg
     2281 tgaattttac catgactggt ttgtggcggc tgaacctgga ttttatgtcc ggcagcaatg
     2341 tggccgatac gacccagtac ttcgatgttg aattttagca gttcataaaa tggcgggcgg
     2401 gttctcccgc ctgcttttaa aaatataatc atgaagtcat ttttttttct gctcctgctg
     2461 ggcagttacc tgcccggttt ggctcaaaac aataataaca gtctgtcggc agacagtacc
     2521 cgaagtattc tgctcagcga agtactggta agcacgaaaa cacgcagtga tcagcaacgg
     2581 atgcttcagt tttaccggag caaccagtct tcaggtcttg aagatattct atcccgtctt
     2641 cctgaaatgt cacttatacg ccgtggtact tatggtatgg aaccggcgat ccgttcttta
     2701 tccggcggac agatcaatgt gctcgtagat ggaatgcgta tccatggtgc ctgtaccgac
     2761 aagatggatc cggccacgat atatattgaa ccaatcaatc tggaacaact acaactgcaa
     2821 accggtacaa atggtttcat aaatgggtca gccatcggag gcacattgaa cctgaaaacg
     2881 gctacccctt cttgccatac cccaaataga ttcagcggaa cgatcagcac tggttttcag
     2941 tcagccgcca aaaactttta tgagtcaatg caactcaact acggaaccgg caaatggtcc
     3001 ttacgtggaa cggccacctg gcgtaaaagc agtgactacc ggagtggtgg cggggaacga
     3061 atcgcttttt cacaatatga aaaagtaaac tattcttttt cggcaaaata tcagctgaat
     3121 caatacaaca gtatcaaggc tgattacatt ggcgataatg gctggaatat cggctatccg
     3181 gccttactca tggatgtagg ttatgcggta gcccggatcg gttcagtcag cctgcaacag
     3241 gaaaacccgg gacaacggct ctatcaatgg tcagctaaat tatatgccaa ccaggttcgc
     3301 cattatatgg atgacaccca ccgtcccaat gtaccgatgc acatggatat gcccggtacc
     3361 agtctgactt atggagtatt tacggaaggc atggctaaac tgaacaacaa acaacagctc
     3421 agggtggtgg ccgacttttc ggcgacaagg ttaaaagctt cgatgaccat gtatgaaccc
     3481 gggcaacgcc cgatgtatat gctgacctgg cccgacaacc gtaaagacca ggctgggatt
     3541 ggtgtgaact gggaatacag ggcagacagt aattggttgc ttcaatcagt tgcccggctt
     3601 gattttatca gtaccgcttt aaccagccag gaagcaaaag atcatatcag tattct
//
LOCUS       DAOPMW010000351         3654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30264_length_3654_cov_8.004168, whole genome shotgun sequence.
ACCESSION   DAOPMW010000351 DAOPMW010000000
VERSION     DAOPMW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30264_length_3654_cov_8.004168"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLU11_13350"
     CDS             <1..907
                     /locus_tag="PLU11_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN60165.1"
                     /translation="GWGLYLWSPLAGTWNLQFNYKNGSLGSPSTTAFTTGVWHHVTAI
                     RNNGTLSMYIDGVLRASGAEGTQTPSYPTSMIAGAMAYLSYSPPRYNPFGGKMDEIRV
                     YNRALSSTEVAQLNAFVLPLKLGQFSATKKTGQVDLIWETLSEENTSHFEIERSSDGN
                     NWTRIGEVSANGNSTQKQSYSYTDPAPSVLTSFYRLKMADKDGIYTYSRVITVTTATE
                     FTGIRLFPNPARETLQVQYEAARSGSLQLMVTDAGGRIVYQSTVSGRSGINTFSVPVQ
                     MLSPGYYQLAILADDRKKSGSFVKE"
     gene            1193..>3654
                     /locus_tag="PLU11_13355"
     CDS             1193..>3654
                     /locus_tag="PLU11_13355"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN60166.1"
                     /translation="MIFKTNIALGALLLLCTCTFAQTNPPRQLPALRINKPIKVDGLL
                     NDSAWKSAPIMSDLVEFRPKVGARENFENRTETYLIYSDEGIYFGGYCYERTRDSIAT
                     ELSGRDGFGTNDYIGIIFDTYYDKINGFEYFVTPLNEQWDAKMSPGDGNNGGEDFGWN
                     AVWESGAVIHDNGWSFEMFIPFAAIRFSKKEVQTWGLNITRRRRKTEQQYTWNPIDPN
                     VNGFLTQEGVWTGITGIKPPLRLQFSPYLSTYVNHFPQSTAGAKDWTSSVNGGMDVKY
                     GINQAFTLDATLVPDFGQVQSDNRVLNLTPFEVKYQENRPFFTEGTELFNKSGLFYPR
                     RIGIDPILTHSIDEYMNGNDRILKNPVESKLINATKISGRTSKGLGVGVLNAVTNNRY
                     ASLENLSDLKQREVLIDPLTNYNIMVLDQSLKHNSSITFTNMNVWRSGSEYDANVSAA
                     QFSLYDKKNTWNVSGKLAMSMLSGAGITDNNRRGYGHSFSFGKSSGRFTFNIKQDLID
                     RKFRGNDLGYFTTTNQVEHYAYAGYNWLKPTRWYNRMFFNFNFYHSYRLMNPAGFQGV
                     NVNVNMNGQLKNLWYVGGMVGYEPKGNNYYEPRVEGRYFKGWSNYFMSAWFETNNAKK
                     YSLSLEAMVIDRSLFNSKRYSFEFSNRYRFNNKLTISHGLSLNPQKRNTGFAAFDGDK
                     IIFGRRDIQTVDNTIGIKYSFNSKMNINTRIRHYWSQVHYDRYFTLRNDGSLARNDNF
                     SGNADYNINFFNIDMVYTWQFAPGSFLNVVWKNAAVNGNQEVTGDYFKNIGRTMQADD
                     NNNLSLKVIYFLDYLQLKKHKK"
BASE COUNT         1011 a          874 c          853 g          916 t
ORIGIN      
        1 tggctgggga ctttatctct ggtccccgct tgcaggcacc tggaacctgc aattcaatta
       61 taaaaatggc agtctcggca gtccctccac caccgccttt accaccggtg tttggcacca
      121 tgtaacggcc atccgtaaca acgggacctt gtccatgtat attgatggtg tattgcgtgc
      181 atcgggagcc gagggtacac aaactccgag ctatccgaca agtatgatcg cgggggccat
      241 ggcctacctg tcctactctc cgccccggta caaccctttt ggtggcaaaa tggatgaaat
      301 cagggtgtac aaccgggccc tcagctctac ggaagtggcg caactcaacg ctttcgtact
      361 gccccttaaa cttggccagt tcagtgccac taaaaaaacc ggtcaggtgg acctgatctg
      421 ggaaacactg agtgaagaaa atacttcgca ttttgaaatt gaaagaagca gtgatggcaa
      481 taactggaca cgcattggtg aagtcagtgc caatggaaat tccacgcaaa aacaatccta
      541 ctcctatacc gaccctgctc cttctgtact caccagcttt taccggctga agatggcgga
      601 caaagacggc atttatacat atagccgggt gattaccgtt acgacagcca ctgaatttac
      661 cggtatccgt ttattcccta atccggcgag ggaaacactc caggtgcagt atgaagcagc
      721 acgttcgggg tccttacaat taatggtgac tgatgcaggt gggaggatcg tttatcaatc
      781 aactgtgtcc ggacgatccg gcatcaatac gttcagtgtg ccggtgcaaa tgctttctcc
      841 gggttattat cagttggcga ttttggctga tgaccgcaaa aaaagcggat cctttgtaaa
      901 ggaataattc taatgcaccc ttcggcacgc tcagcatgac aacgggctgt ccttgtcatg
      961 ctgagcattc cgaagcattg ccgaagcatc ccaggcaatg attatttccc aataaaaagt
     1021 taacgcccct gtattttaac cactcgtcag gtcatacatg ccggtcagat aaatccaaaa
     1081 cctattccct ttttaccgct atacttttac cgtacgaaag acttagtagt tctttcccga
     1141 atcagtaaca aaacctgctg cttcaacgtc ctgactactt taaagtatag ccatgatttt
     1201 caaaaccaac atcgccttag gggctctcct gctcctgtgt acctgcactt ttgcccaaac
     1261 taacccaccc cggcaattgc cggctttacg cattaacaaa ccgatcaaag tagacggcct
     1321 gctgaatgat tccgcctgga aatcggcgcc cataatgtcg gacctggtgg agttcaggcc
     1381 caaagtcggc gcccgggaga actttgaaaa ccggaccgag acttacctga tctatagtga
     1441 tgaaggcatt tattttggcg gatattgcta tgaacgcacg cgtgacagca tcgccaccga
     1501 attatccggc cgggatggtt ttggtaccaa tgattatatc gggatcattt tcgacaccta
     1561 ttacgataag atcaacggct ttgaatattt tgtcaccccc cttaacgaac aatgggatgc
     1621 taaaatgtcg cctggtgacg gaaacaacgg gggcgaggat tttggatgga atgctgtttg
     1681 ggagagcggc gccgtgatcc atgataatgg ctggagtttt gaaatgttca tcccctttgc
     1741 cgccatccgg ttcagtaaaa aagaagtgca gacatgggga ctgaatatca cccgccgccg
     1801 tcgaaaaacg gaacagcagt atacctggaa cccgattgat ccgaatgtaa acggcttcct
     1861 gacacaggag ggggtctgga caggtattac gggcattaaa cccccactgc ggttgcagtt
     1921 ttctccttac ctgtctactt atgtgaacca tttcccgcaa tccactgccg gggcaaaaga
     1981 ctggacctcg tcagtcaatg gcggaatgga tgtgaaatac ggaatcaacc aggcttttac
     2041 ccttgatgcc accctcgtac ctgatttcgg acaggtgcaa agtgacaacc gggtactcaa
     2101 cctcactccg tttgaagtga aataccagga aaaccgcccc tttttcacgg agggtactga
     2161 actgtttaat aagagtggtc tcttttatcc gcgacgtatc ggaattgatc cgatacttac
     2221 tcacagcatt gatgaatata tgaatggcaa tgaccggatc ctgaaaaacc cggtggaatc
     2281 caaactgatt aatgcgacaa aaatttccgg cagaacctca aaaggcctgg gggtgggtgt
     2341 actcaatgcg gtcaccaata accgctatgc ttcactggaa aatttgtctg acctgaaaca
     2401 aagagaggta ctgatcgatc cgctcaccaa ttacaatatc atggtactgg atcagtcgtt
     2461 gaaacataat tccagtatca ctttcaccaa tatgaatgtg tggcgcagcg gatccgaata
     2521 cgatgccaat gtaagcgcgg cacagttcag tctgtatgac aaaaagaaca cctggaatgt
     2581 aagtggtaaa ctggcgatga gtatgttgtc cggagccggg ataacggata ataaccgccg
     2641 gggatacgga catagctttt cgtttggcaa atccagcgga cgattcacct tcaatataaa
     2701 acaggacctg attgaccgga aattccgggg gaatgacctg ggctatttca ccaccaccaa
     2761 ccaggttgaa cattatgcgt atgccggcta caactggctt aaacccacgc gttggtataa
     2821 ccggatgttc ttcaatttta acttctatca ttcctaccgg ttgatgaatc cagctggctt
     2881 ccagggggtg aatgtaaatg tgaatatgaa cggacaactg aaaaacctct ggtatgtggg
     2941 agggatggtt ggctatgagc cgaaaggcaa taactattat gaaccgcggg tggaaggacg
     3001 ttatttcaaa ggctggtcga attatttcat gtcggcatgg ttcgaaacca ataatgcaaa
     3061 aaaatacagt ctctcattgg aggcaatggt catcgaccgt tccctgttca acagcaaacg
     3121 ttattcattt gaattttcca accggtaccg atttaataat aagctgacca tttcgcacgg
     3181 cctctctctg aatccgcaga aaaggaatac cggctttgct gccttcgatg gtgataagat
     3241 tattttcgga agaagggata tccaaaccgt agacaataca atcggcatca agtattcctt
     3301 taacagtaag atgaatatca atacccgtat ccgtcattac tggagccagg tgcattatga
     3361 ccggtatttt accctgcgca atgatggctc actggcccgg aatgacaact ttagcggcaa
     3421 tgccgattac aatatcaact tctttaatat tgatatggtg tatacctggc agtttgcccc
     3481 gggcagtttt ctgaatgtgg tctggaaaaa cgctgcagtc aacggtaacc aggaagtgac
     3541 cggcgattat tttaaaaaca tcggtcgtac gatgcaggca gatgataaca ataacctgtc
     3601 actgaaagtg atttactttt tggattacct gcagttgaaa aagcataaga agaa
//
LOCUS       DAOPMW010000352         3654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30265_length_3654_cov_7.326757, whole genome shotgun sequence.
ACCESSION   DAOPMW010000352 DAOPMW010000000
VERSION     DAOPMW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30265_length_3654_cov_7.326757"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLU11_13360"
     CDS             <1..357
                     /locus_tag="PLU11_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN60167.1"
                     /translation="VDQLNRWQNPGDDTDVPQLRLGYGNGIGASSRYVENASYVRLKN
                     ITVAYNVDSKLLKKLKIRSARFYLTGVNLATFTKYTGWDPEVNTDYRAGNRNQGGDFY
                     AAPQIKSMTFGINLGF"
     gene            378..1724
                     /locus_tag="PLU11_13365"
     CDS             378..1724
                     /locus_tag="PLU11_13365"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN60168.1"
                     /translation="MKKYISIVFALGVLFTSCDKKLDIFPEDSVADEIALTTDVNVKK
                     VLNGAYDAISSASLYGGDLFLFSELLAANGEIRWEGTFNQPREIWLKSMLTTNSYVRD
                     SWLDAYNTINVCNNILGAINVVNTADQNRVKGEALFMRGAMFFELVKFFAKPYSAGSV
                     TSNPGIPLILTPTRGIDQSSFVARSTVEQVYTQILADLTEAENLLPNTNSVYATKPAA
                     SAMLSRVYLQMERFADARDAANRGITVATANGKSLAASYAAAFNNSSNVAEYLLAIQV
                     SAQDGSNDMHLFWSIPSYGGRDGDVSIQASHTALYSSGDARLDLFYSGAGDVRSGKWQ
                     QQYKVLPVIRLAELYLTRAEANLRSSTSIGDSPVNDVNRIRNRAGLTSLGSVVLADIL
                     AERKLELAHEGHAVHDLKRLKQSADGFAYNADKMVFPIPQREVDASRGTLAQNSGY"
     gene            complement(2063..3619)
                     /locus_tag="PLU11_13370"
     CDS             complement(2063..3619)
                     /locus_tag="PLU11_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPN60169.1"
                     /translation="MNKELIINAAPQGVEIALLEDKKLVELHNEKTDARFAVGDLYLG
                     KVKKLIPGLNAAFVDVGFEKDAFLHYTDLSPYAKSLLKFTTMCMNHKSEQGLDFGKFT
                     IEPEIVKTGKINEVLGGKPHILVQILKEPIAAKGPRLSCEISLPGRFVVLTPFNDIVA
                     VSRKIHSSEERKRLQKIVEAIKSKNFGVIVRTAAEGKNTAELHEDLSALVEMWETIQK
                     NLKGAVAPAKILSEQTKTTSILRDLLNEDFNRIVVNDKTIFADTKSYIQRIAPDKADI
                     ISFYQNGSTIFDSFGITKQVKSSFGKTVNLNSGAYVIIEHTEALHVIDVNSGYKSVSN
                     NQEQNALETNLEAAEEIARQLRLRDLGGIIVVDFIDMKLPDNKRKLVERMEEFMSTDR
                     AKHAVLPISKFGLMQITRQRMKPEMNINTQEVCPSCNGTGKISSTLLLEDEIEKNLSY
                     LITHKNGNLKLVVHPILYSYLSKGWPWNTKMAKWKRKFGQKTRLEQDTNYHLTEYKFF
                     DKHDEEIKMN"
BASE COUNT          892 a          839 c          865 g         1058 t
ORIGIN      
        1 gttgatcagc tgaaccgctg gcagaatccc ggtgatgata ccgatgtgcc ccagctgcgt
       61 ctcggctatg gaaatgggat cggtgcttcc agccggtatg tggagaatgc ttcttatgta
      121 cgtctgaaga atatcacagt ggcgtataat gtggacagca agctgctgaa aaaactgaag
      181 atacgttctg cccgttttta cctgaccggc gttaacctgg ctactttcac caagtatacc
      241 ggatgggatc ctgaagtgaa tactgattac cgtgccggaa accgtaacca gggtggtgat
      301 ttctatgcag ccccccagat caaatccatg acatttggta ttaatctcgg attctaatcc
      361 attaaatgaa atcaattatg aaaaaatata tttcgatagt ctttgcactt ggagtgcttt
      421 tcacttcctg tgataaaaag ctggacattt ttccagaaga cagtgttgcg gacgagatag
      481 cgctgactac tgatgtaaat gtgaaaaaag tactgaatgg ggcctatgat gcaatcagta
      541 gcgccagcct ttatggtggt gacctgtttc ttttttcaga attactggca gccaatggtg
      601 aaatccgttg ggaaggtact tttaaccagc ccagggaaat ctggctgaaa tccatgctca
      661 ctaccaatag ttacgtacgt gattcatggc tggatgcata caacactatt aatgtttgca
      721 ataatattct tggtgccatc aatgtggtaa acactgctga tcagaacagg gttaaaggtg
      781 aagcgttgtt tatgcgtggt gccatgttct ttgaactggt gaagtttttt gcaaaaccat
      841 atagtgctgg ttctgttacc agcaatcccg gtatacccct gattcttact cccacccggg
      901 gtattgacca gagttccttt gtggcccgca gcaccgtgga gcaggtgtat acccagatcc
      961 tggccgattt aaccgaagcc gaaaatctgc tgcccaatac caattctgtg tatgcaacca
     1021 agcctgcggc atctgctatg ctttcacgtg tgtacctgca gatggagcgt ttcgcagatg
     1081 caagggatgc cgctaatcgt ggcatcactg tagcaacggc caatgggaaa agcctggctg
     1141 cttcatatgc cgctgcattc aacaacagtt ccaatgtagc tgaatacctc ctggccatcc
     1201 aggtaagtgc acaggatggg tccaacgata tgcacctgtt ctggtctatt ccttcttatg
     1261 gtggacggga tggtgatgta tctatccagg ctagtcatac cgccctttac agcagcggag
     1321 atgcccgtct tgacctgttt tactccggtg ccggagatgt acgcagtggt aaatggcaac
     1381 aacagtataa agtactgccg gttatccgcc tggcagaact ttaccttacc cgtgctgaag
     1441 ctaatttacg ttccagcact tctattggag atagcccggt aaatgatgtc aaccgtatcc
     1501 gtaacagggc gggtttgact tctttaggtt cagtggtact ggctgatatc ctggctgaaa
     1561 gaaaactcga actggctcat gaaggacatg cagtgcatga cctgaagcgc ctgaagcaaa
     1621 gtgcggatgg atttgcctat aatgccgaca aaatggtatt ccccattccc cagcgggaag
     1681 tggatgcctc ccgtggcacc ttggcacaga attccggata ttaatagtta atcatcatct
     1741 ttatagaaga ggctgtcttg aaaagggcag cctctttttt tgtgggggga ggggggggga
     1801 tgagtaatga gtaatgagta cagggggaaa gtcattactg taagaggctt tttccaaagc
     1861 gggttgtgtc aaaattaagt tcagtactac cagctactca ttactcattc cccctctaaa
     1921 cctgcatctg ctatgaaagt attttcccgg gctactcatt actgattact cattactcat
     1981 tcccccgctc agcctgcttc tgttataaac caaagggccg ttacttttca gcaacagccc
     2041 ttaaaaaaaa tttaaatccc ctttaattca tctttatttc ctcatcatgc ttatcgaaaa
     2101 acttatactc ggtaagatgg taattggtgt cttgttccag tcttgtcttc tgtccgaatt
     2161 tacgtttcca cttggccatc ttggtattcc agggccagcc tttggacagg taggagtata
     2221 atatcgggtg taccaccaat ttgaggttgc cgtttttatg ggtgatcagg tagctgaggt
     2281 tcttttcaat ttcatcttcc agtaaaaggg tggaagagat ttttccggta ccattacaac
     2341 tcgggcagac ttcctgggta ttgatattca tttccggctt catccgctgg cgtgtgatct
     2401 gcatcaggcc aaacttggag atcggcagta ccgcatgctt ggcacgatcg gtactcatga
     2461 actcttccat tctttcgacc agcttccgtt tgttgtcggg cagcttcata tcgatgaagt
     2521 ctactacaat aatacctccg aggtcgcgca ggcgcagctg acgggcaatt tcttctgccg
     2581 cttccaggtt ggtttcgagg gcattttgct cctggttatt gctgacactc ttataaccgc
     2641 tgttaacgtc aataacatgc agggcttcgg tgtgttcaat gatcacatag gcgccgctgt
     2701 tgaggttgac cgttttgccg aaagaggatt tcacttgttt ggtaatgccg aaagaatcga
     2761 aaatggtgga tccgttctgg taaaaggaaa tgatatccgc tttatccggt gctatgcgct
     2821 ggatatagct tttagtatct gcaaaaatcg ttttgtcatt gaccacaatc cggttgaagt
     2881 cttcgttgag cagatcgcgc agaatactcg tagtcttggt ctgctcactc aggatcttgg
     2941 ccggcgccac agctcctttc agatttttct gaatggtttc ccacatctct acgagggcag
     3001 aaagatcttc atgaagttcg gccgtgttct ttccttccgc cgcggtccgt acaatcacgc
     3061 cgaaattctt tgatttgatg gcttccacaa tcttctgcag gcgttttctt tcctccgaag
     3121 aatggatctt ccgggaaacg gctacaatat cattaaaggg agtgagtacc acaaaacggc
     3181 cgggcaggga gatttcacag ctcagacggg ggcctttggc agcgatgggt tctttgagaa
     3241 tctgaacgag aatatgcggt ttaccaccca gtacttcatt gatcttgccg gtctttacga
     3301 tctcgggttc aatcgtgaat ttgccaaaat ccagtccctg ctcagactta tggttcatgc
     3361 acatggtcgt gaacttcagg agggatttgg cataagggct caggtcggta taatgcagaa
     3421 aggcgtcttt ttcaaaaccc acatctacaa atgccgcgtt cagtcccgga atcagttttt
     3481 taacttttcc gagatacagg tctcccacag caaaacgtgc atccgttttt tcgttatgca
     3541 gttctaccag ctttttatct tccagcaggg cgatttcaac gccctgggga gccgcattaa
     3601 taattagttc tttgttcatt acagcttaca taaatgaaac accttagtgc tgta
//
LOCUS       DAOPMW010000353         3636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30446_length_3636_cov_6.314158, whole genome shotgun sequence.
ACCESSION   DAOPMW010000353 DAOPMW010000000
VERSION     DAOPMW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30446_length_3636_cov_6.314158"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..907)
                     /locus_tag="PLU11_13375"
     CDS             complement(80..907)
                     /locus_tag="PLU11_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60170.1"
                     /translation="MSDRVWTNPLSQDAGRRRTELCKAQDFHQDWYHRWCAEMKETPR
                     FHRKQWEYAYNMQALWERGCIREGSRGLVFAVGTEPGPSIFAGYGCQILATDIMPERG
                     KEKGWDNGQLCFGLESLNARQLCSDKILQEKVSYRPLDMNQIPDDITGFDFNWSSCSF
                     EHLGSIEKGLAFLKNQLKTLKPGGWAVHTTEYNISSNQATQDLNETVIFRQRDIVQVV
                     GELRAEGHYVEETDFSIGGMPEDFQVDLTPHQQEIHVKLQVGPFVVTSLGLIIRKKQ"
     gene            complement(909..2000)
                     /locus_tag="PLU11_13380"
     CDS             complement(909..2000)
                     /locus_tag="PLU11_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN60171.1"
                     /translation="MSTPPKNIILDCDLMKYPNSGLYHYCLNLGNEMSELLTDAADLG
                     ISFYTPPSAKNDFNRPSSAIVERKGLLRYFKPFLHRCSIWHAPFQSGRIVPDKRRFPE
                     LKVLLTIHDLNPLHEGKSNEEQEKSLAHTQSLIQKADALVCISEFTKSDVLNYCDTGN
                     KPVHVIHNGSHQVHTPSLGDQSYRPSRPFLFGMGYVNRKKNYHVLLPLLENESIEMVI
                     AGRLDEADYVNSILNTAESMGVSDRLRLTGPVSEGEKAWYYQHCLAFVHPSLAEGFGA
                     PVVEVMQFGKPLFLSDRTSLPEIGGDAAFYFSSFEKEHMHHIFRQGMEKYNSNGLADH
                     IRKRGAAFDWKQKAKEYLAVYRSMIGQSS"
     gene            2185..3243
                     /locus_tag="PLU11_13385"
     CDS             2185..3243
                     /locus_tag="PLU11_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN60172.1"
                     /translation="MQKIIFDCERMKYPDTGLYHYCMNLGRYLEKNINLREEQLLFYT
                     PPGEQDWSYSRNNHFTQHPLHKFRLPDLGSAAIWHATYQNTHYMPLRNKKVKVVLSIH
                     DLNFLYDEKKSAAKKQKYLQYLQQLINRADALVCISEFSRQDVLLHCEVGNKPLFVIH
                     NGSNLLETPSLNIKSYRPRKPFLFSIGVVHRKKNFHVLLPLLKQNTMELLIAGRHDDD
                     HYISFIKEKSRELGVADNLHLLGRVTEQEKSWYFNNCRAFAFPSISEGFGLPVVEAMS
                     CGKPLFLSDRTALPEIGGDVSFYFRDFNPDHMQEVFNAGMDSYTEQNMCKKIQERGTH
                     FNWHQSAKEYLSVYRSLY"
     gene            3264..>3636
                     /locus_tag="PLU11_13390"
     CDS             3264..>3636
                     /locus_tag="PLU11_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60173.1"
                     /translation="MNVYKRLIIYARPLRWFLVPFFIFTLLAVFFTVFQYALIIPLLN
                     VLFENSPSGNTTTLPAFSFSGHFFKDFFYYQVYRLKAIHPAYALYFITAVLVGAVLLA
                     NLFRYLAQRTLIRARTLLVKRI"
BASE COUNT          925 a          866 c          821 g         1024 t
ORIGIN      
        1 aaattaaaga tttttaactg tggaaaaggg gttgaaaacc cagcctgaaa aaggggcttt
       61 ccggctctga ggtcctgttt tactgctttt tacggataat cagtcccagg gaagtgacta
      121 caaaggggcc cacctgcagt tttacatgga tttcctgttg gtgcggggtc aggtctacct
      181 ggaaatcctc aggcatcccg cctattgaaa aatcggtctc ctccacataa tggccttccg
      241 cccgcagctc tccgaccacc tgtactatat cccgctgcct gaagatcacc gtttcattca
      301 ggtcctgggt ggcttgatta ctggaaatat tatattccgt ggtgtgaacc gcccagccac
      361 cgggtttcag cgttttaagc tggtttttta gaaatgccag tcctttttca atactgccga
      421 ggtgttcaaa ggagcaactg gaccagttaa agtcaaatcc ggtaatatcg tccggtatct
      481 ggttcatgtc caacggacgg taactgactt tttcctgtaa aatcttatca ctgcagagtt
      541 gccgggcatt cagggattcc agtccgaaac agagctgccc gttatcccag cccttttcct
      601 tcccccgttc aggcataata tcagtggcca gaatctgaca gccataaccg gcaaaaatag
      661 agggacccgg ttcggttccc acggcaaata ccagtccgcg actcccctcc cggatacagc
      721 cccgctccca taatgcctgc atattgtagg catactccca ttgcttgcgg tgaaaccgtg
      781 gagtttcttt catttctgca caccagcgat ggtaccagtc ctggtgaaaa tcctgggcct
      841 tgcacaattc tgtccttcgc cttccggcgt cctgacttaa ggggtttgtc cagactctat
      901 ctgacatttc aggaggattg accgatcatg gaccggtata cagcaaggta ctcttttgct
      961 ttttgtttcc agtcaaaagc agccccgcgt ttccggatat gatccgccaa cccgttgctg
     1021 ttatattttt ccatgccttg ccggaaaata tgatgcatat gttccttttc aaaagacgaa
     1081 aaataaaagg ccgcatcccc gccaatctcc ggtaatgatg tccggtcaga aaggaataat
     1141 ggtttgccaa attgcatgac ctccaccacc ggggcaccga acccttcggc cagtgaggga
     1201 tgtacaaaag ccaggcaatg ctggtagtac cacgcttttt cgccctcgct aaccggcccg
     1261 gtgaggcgga gacggtctga tacacccatg ctttctgcgg tattcaggat actgttcacg
     1321 taatctgctt catccagtct gccagctatg accatttcaa tggattcatt ttccagcaat
     1381 ggcaataaaa catggtagtt ttttttgcga ttgacatagc ccataccaaa caaaaatggt
     1441 ctggatgggc ggtaagattg gtctccgagt gacggtgtgt gtacctgatg agaaccattg
     1501 tgaatcacat ggacgggttt attaccggta tcgcaataat tcaggacgtc ggatttagta
     1561 aattcagaaa tacaaaccag tgcatccgct ttttggatca gggattgggt atgtgccaga
     1621 cttttttcct gttcttcatt cgatttgcct tcatgtaaag gattcagatc atgaatagtg
     1681 agcagtactt tcagttccgg aaaccggcgc ttgtccggaa cgatcctacc cgactgaaaa
     1741 ggtgcatgcc agatactaca acgatgcaga aagggtttga aataacggag cagtcctttt
     1801 ctttccacga tggcggatga cggcctgtta aaatcatttt tggcggaggg aggggtgtaa
     1861 aatgaaatac caagatccgc cgcatcggtc agtaattcgc tcatttcatt ccccaggttg
     1921 aggcagtagt gataaagtcc tgagttgggg tacttcatca ggtcacaatc cagaataata
     1981 tttttgggag gggttgacat gctataaagg ttaaaattag tgtacgccgg gcaatcgggt
     2041 gtaaccatga attttatcct tgccggagtt acatccggat cattatgatt taccaaccat
     2101 atggatgggg cactatattt gacgtttaat ggcaaatgac cctgatcttg aataatcaac
     2161 ggcttcattt atataatcag acctatgcaa aaaatcattt ttgactgtga gcggatgaag
     2221 tacccggata ccggtttgta tcattattgt atgaacctgg gacggtatct ggaaaaaaac
     2281 atcaacctgc gggaagaaca actcctgttt tataccccac cgggtgaaca ggactggtct
     2341 tattccagga acaaccattt cacccagcat cctttacata aattccgctt acctgacctg
     2401 ggtagtgccg ctatctggca tgccacgtac cagaacaccc attatatgcc cctgcggaat
     2461 aaaaaagtga aagtggtgct cagtattcat gacctgaact tcctgtatga tgaaaaaaaa
     2521 tcagcagcaa aaaaacagaa gtacctgcag tacctgcagc agctcatcaa ccgggccgat
     2581 gcgctggttt gtatttctga attcagccga caggatgtgt tactgcattg tgaagtgggc
     2641 aacaaacccc tttttgtaat ccataacgga agtaacctcc tggaaacccc ttctttaaat
     2701 atcaaatcct accggccccg taaacccttt cttttttcca ttggcgtggt gcaccggaag
     2761 aaaaatttcc atgtactcct tcctttgctg aaacagaaca ccatggaact gctgattgcc
     2821 ggcaggcatg atgacgacca ttatatatct tttattaagg aaaaatccag ggaactgggg
     2881 gtagctgata acctgcattt gctggggcgg gtgacggaac aggaaaaatc ctggtacttt
     2941 aataactgcc gggcatttgc ttttccgtcg atatcggaag gttttggttt acccgtggtg
     3001 gaagcgatga gttgtggaaa gccgctgttt ctgtcagacc gtaccgcatt acctgaaatc
     3061 ggcggggatg tttcctttta tttcagggac tttaatcccg accacatgca ggaagtattt
     3121 aatgcgggta tggattcgta tactgaacag aatatgtgta aaaaaattca ggaacggggt
     3181 acccatttta actggcacca gtcggcaaaa gagtatcttt ctgtctatcg ttccctttac
     3241 taataaaaag tacagcctcc tgtatgaatg tttataaacg cctgataatt tatgcacgtc
     3301 ccttgaggtg gttcctcgtg ccctttttta tttttaccct gctggctgtt ttttttaccg
     3361 tctttcagta tgcactgatc attccgttgc tgaatgtatt gtttgagaat tcaccatcag
     3421 ggaataccac cacattaccg gccttttctt tttccgggca ttttttcaaa gacttttttt
     3481 attaccaggt ataccggtta aaggctatcc acccggctta tgcgctttat tttattacag
     3541 cggtactggt gggagctgta ttactggcca acctgtttcg ttatctcgcc cagcgaaccc
     3601 tgatccgggc ccgcaccctg ctggtcaaaa gaatcc
//
LOCUS       DAOPMW010000354         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30597_length_3624_cov_7.962735, whole genome shotgun sequence.
ACCESSION   DAOPMW010000354 DAOPMW010000000
VERSION     DAOPMW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30597_length_3624_cov_7.962735"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLU11_13395"
     CDS             complement(<1..565)
                     /locus_tag="PLU11_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60174.1"
                     /translation="MDREQRREERRQRREDYRMRWEEQRQQYMDNAPRHGHVWTGLFI
                     VLIGVAALLHASFDDLPAWIFSWQMFLIALGLFLGLKHGFRGAAWIVLIAIGSFFMLQ
                     EFYPELAIRRYFWPLLLIAGGAFIIIRPRRKSWEQCVRKENKMNTGTTAGTATNIGAT
                     GSTDNFMGSGETWSKDDFVDSTSIFGGD"
     gene            complement(832..1128)
                     /locus_tag="PLU11_13400"
     CDS             complement(832..1128)
                     /locus_tag="PLU11_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60175.1"
                     /translation="MKQHLHISAAFDQPELVQNLVERLAYRRYDAGYDKEIMPPGCKA
                     LETSSTEAFVSGTMGIESLNKQEHYNMPFITCFMFTCIKGKEEPYQLIWSSSLN"
     gene            complement(1634..2017)
                     /locus_tag="PLU11_13405"
     CDS             complement(1634..2017)
                     /locus_tag="PLU11_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60176.1"
                     /translation="MKKLLNSLLLLSSFFGYLEWPPDNHGFIFQLEAEILQIAKTNAS
                     SVAHPFILLPFIGQVLLLVSLFQTPPKRWLTLTGLLCTGLLMLLLFLIGLLTAHVGIV
                     ASTLPYLLISFFVVRINFQKKGRLS"
     gene            complement(2042..2947)
                     /locus_tag="PLU11_13410"
     CDS             complement(2042..2947)
                     /locus_tag="PLU11_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60177.1"
                     /translation="MRKFIFFSGFLLFLSFYQTANAQGCSDAGFCSMSSFKPQSNAGV
                     KYQDRLRIGFSAGKADNDIFITGQYLELNKSISDQFSVDTRITSLSQSGTPASTFGFS
                     DIYINGNYKTGKVGLTLGAKIPLNPADRTKNNLPLPMDYQSSLGTFDLLAGIGFTIKK
                     LQFNTALQQPLTQNKNRFMATDYPNGSPFQQFQSTSRFKRKGDVLLRLSYPLNLGSKL
                     TFTPGVLSIYHLGTDTYRDNMGIQKEIIGSDGLTLNSNLFFDYAINNRQGLQLSFGAP
                     FLVRDTRPDGLTRKFVAGLEYSIRF"
BASE COUNT         1030 a          769 c          872 g          953 t
ORIGIN      
        1 tatctccgcc gaagatggag gttgaatcga caaagtcatc cttgctccag gtttctccgg
       61 aacccataaa attatcggta ctaccggtgg caccaatatt tgtagccgta cctgcagttg
      121 tgccagtatt cattttgttt tctttccgta cacactgctc ccaggatttg cgacggggcc
      181 ggataatgat aaaggcgccg cctgcaatca gtaataaggg ccagaaatag cggcgaatag
      241 cgagttcggg gtagaactcc tgtaacataa agaagctgcc gatggcgatc agcacgatcc
      301 acgcggctcc ccggaacccg tgcttgagcc cgagaaaaag accaagagcg atcaggaaca
      361 tctgccagct gaagatccag gcgggcaggt cgtcaaaact ggcatggagc agggcggcca
      421 caccgatcag gacaataaag aggccggtcc agacatgccc atgccggggg gcattatcca
      481 tatattgctg acgttgttct tcccagcgca tccggtaatc ctcccgtcgc tgtcgtcttt
      541 cttctcgtct ttgttcgcgg tccatacaat caattttaac caaaactaag gtctgagacc
      601 ctttggggca agcgggaatg ggctggagca ggggaattat cggtgaatgg cgggaaacct
      661 ggggtgaaag acaaagaacc aagatgccaa gacaccaaga tgccaaagaa gaaccaaaca
      721 acaaaaccca aaaaaggacc aataaagaaa aaatccaaag cctccttaga agggtttgga
      781 ttttacttct tgatgcttat ttggcatctt ggtgtcttgg catcttggat cttagtttag
      841 agagctgctc caaatcaact gataaggctc ctccttaccc ttgatgcagg taaacataaa
      901 acaggtaata aacggcatat tatagtgttc ctgtttattg aggctttcaa tccccatggt
      961 gccacttaca aaggcttcgg tggaagaggt ttccagggcc ttgcagccag gcggcatgat
     1021 ctctttatca taaccggcat cataacggcg gtaggcgaga cgttctacga ggttctgcac
     1081 cagctcaggc tggtcgaagg cggcggaaat gtggaggtgc tgtttcatat cactggtttt
     1141 tgggccagcc ccgttactca cattccaacc gcactggctg tacttattta ttagatgcag
     1201 gattggttga attggttact aaggaaatgt gaaagtcggg tgtcagatgt cagatgtcag
     1261 ttgccaggac tttcttcgga gtttgccttt ttatcccggg cggagcgatg catagttgca
     1321 taagtgaaaa gcacaattat tgtagttgtt acacgacaga gtccttgtca ttccgtttga
     1381 agcattgttc aattttaatg ggaaaattta aaagaaacct tgtcatcccg aagtttaccc
     1441 tgagtttgcc gaagggaagg agggataaga ggattctttt cccatcgaat gttgattccg
     1501 aagcaaagcg aggaatcttg aatcctgtca tttggttaaa ttgatgcaaa gttttcaatt
     1561 taacctcaat gatatcttta ccaggagaat tacttgtgaa cgcgtatact taaaaaccag
     1621 tagtcggttt attttacgac aacctccctt ttttctgaaa attaattcga accacaaaaa
     1681 aggaaatcag taaataggga agtgtggaag ccacgatccc gacatgcgcg gtaagcaacc
     1741 caatcaaaaa aagtaaaagc atcagcagtc cggtacaaag cagtccggtc aaagtgagcc
     1801 agcgttttgg tggtgtttga aacaggctga ccagtaacag cacctgcccg ataaaaggca
     1861 ataggataaa aggatgtgcc acggagctgg cattggtttt tgcgatctga agtatttctg
     1921 cttccagttg gaaaataaat ccatgattat ccggcggcca ttccaggtag ccgaaaaagg
     1981 aactaagtaa caggaggctg ttgagaagtt ttttcatccg acagcgcaaa gataaacaga
     2041 atcaaaaccg gatgctgtat tccaatccag ctacgaattt ccgcgtaaga ccatccggtc
     2101 gcgtatctct taccaggaat ggagccccga aacttaattg taatccttgc cggttattga
     2161 tggcatagtc aaaaaacaga ttactattca gggtcaatcc atccgagcca atgatctctt
     2221 tttgaatgcc catattatcg cggtaagtat cagtgccaag atgataaatg gataaaacac
     2281 ccggggtaaa tgtgagtttt gatcccaggt tcagcggata agataatcgg agcaatacat
     2341 ctcccttgcg tttaaagcga ctggtggact gaaactgctg aaacggagaa ccgtttggat
     2401 aatcagtagc cataaacctg ttcttgttct gggtcagcgg ttgttgaaga gcggtgttaa
     2461 actgtaattt ctttatcgta aagccaatcc cggcaagcag gtcaaacgtc cccaggctcg
     2521 attgataatc catcggtaac gggagattat ttttagtacg atctgccggg ttcagcggta
     2581 ttttagcacc gagtgtcagg ccgacctttc cggttttata attgccatta atataaatat
     2641 ctgaaaagcc aaaagtgctt gccggagtgc cggactgtga cagggaggta atacgggtat
     2701 ctacagaaaa ctgatcactg attgatttat tcaattcgag gtactgtccg gttatgaata
     2761 tatcattatc tgctttgcca gccgaaaaac caatccgcaa ccggtcctgg tacttaacgc
     2821 ctgcgtttga ctggggttta aatgaactca tactgcagaa tccggcatcg ctgcaccctt
     2881 gcgcattagc cgtttgatag aaagacaaaa agagaagaaa gcccgagaag aaaataaatt
     2941 ttcgcataaa cagttttttt tcaatgtagt caaatataat acttgcactg tttatgaacg
     3001 gactaaatgc atgggaggta tttcgggagg gtgttaaata attgtcaaga tgagtttcgc
     3061 tcagacttcg gagatgcctc ctgacatctg acacccgaca cctgacacct gaaataaacc
     3121 cggaacaact gcttcaatgg ttactgttat gattgatcag gatggagggc aggataggca
     3181 gtcgctccgg gctgtatctt agggtatatt tatcgggtac gtgtacctgc tgacagtaac
     3241 cggccggcag ggtcaggtat tttacacgga attattattc cgcgcatgac gaacatattt
     3301 gatacgggaa gggtagcagg atgggagggg attaagatta cagggtaaag gtaaaccggc
     3361 cagacataca accctatacc actttcttgg tattttttgg acagggagtc actgttaaag
     3421 ggggatgact actacatttt atgtatttcc tacacaatgc tgccgggcta gttttgataa
     3481 aaatcatcaa ttatgtgtaa atcatttttt caaccctttg cttttttgtt attaaccgtt
     3541 ttagcagcag gtctttcgct gaccagttgt ggtaaggata attccggcgg taataataat
     3601 tccgccaccc cattctggaa aggg
//
LOCUS       DAOPMW010000355         3621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30641_length_3621_cov_11.627033, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000355 DAOPMW010000000
VERSION     DAOPMW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30641_length_3621_cov_11.627033"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLU11_13415"
     CDS             <1..369
                     /locus_tag="PLU11_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN60178.1"
                     /translation="YDDEQLKFRFMTDLTGVHYPNQTGRELAVVYHLHNLVDNIRLRF
                     KVFTDINQPDIYTATGLFSAANWMERETYDFFGVNFVGHPNLKRILNVDEMDYFPLRK
                     EFPLEDQTRIDKDDEMFGRG"
     gene            494..1717
                     /locus_tag="PLU11_13420"
     CDS             494..1717
                     /locus_tag="PLU11_13420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPN60179.1"
                     /translation="MSEHHIKLPEGSIEKTTTTLNLGPTHPATHGVMQNILELDGERI
                     VSTEQTIGYIHRAFEKLAERRPLAQITTLTDRLNYCSSPLNNMGWHLTCEKLLGVKTP
                     KRVDYLRVIIMELARISDHLICNSIVGVDAGAYTGFLYVMQYRELIYEIYEEVCGARL
                     TTNIGRIGGFERNFNDIAFQKLEKFLREYPAVLKEFENLFQRNRIFMERTIGTGAISA
                     DRALNYGFTGPNLRAAGVDYDVRVHSPYSSYEDFQFNIPVGTTGDNYDRWLVREKEMW
                     ESLSIIEQAYQKVQEFKGAEADVFHADVPEYYLPPKAEVYTKMEALIYHFKIIMGETN
                     IPAGEVYNSIEAGNGELGFYLVSDGGRSPYRLHFRRPCFVYYQAYEELIKGSMLSDAI
                     MTMSSLNLIAGELDA"
     gene            1974..2450
                     /locus_tag="PLU11_13425"
     CDS             1974..2450
                     /locus_tag="PLU11_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN60180.1"
                     /translation="MNEVQVIISRYPQGKQKSALIPVLHIAQQEFGGWLSTETMDYVA
                     SVLQLKPIEVYEVATFYSMFNLKPVGKYMFEVCQTGPCMLNGSDDIVKYIFDTLGIKP
                     GETSADGLFTLKTVECLGACGYAPMMQQGKYYREHLTKEKIDAIIAECRNTAAQQN"
     gene            2450..2887
                     /locus_tag="PLU11_13430"
     CDS             2450..2887
                     /locus_tag="PLU11_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN60181.1"
                     /translation="MKKIITILFLSMGLSGIAQSKDQVKVLAASKQFQETVFGSKDSL
                     ILERLFAATVHYEHSSGKVENRAEAIRGISRNKSVYVMIPDPSPYKISKQGDTLVVNT
                     VLKAVEQKADGSKTDLNLSIDLYWIKEGKQWKLTKRVATKRHE"
     gene            2884..3303
                     /locus_tag="PLU11_13435"
     CDS             2884..3303
                     /locus_tag="PLU11_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN60182.1"
                     /translation="MKFFVTIVLLCWQMAGLAQTDEVKLTSTLKEFHQALVNKNKVSI
                     NQQTDKALSYGHSNGWVETKKELIEHLESGYMEYNSFREDSIKVEVNGNVAHARFIAE
                     INATMGGKEFTFQLRVLEVWVKKGKRWVLFARQAVRK"
BASE COUNT         1089 a          719 c          930 g          883 t
ORIGIN      
        1 tacgatgatg agcagctgaa attcaggttc atgaccgatc tgaccggtgt tcattatccg
       61 aatcagaccg gacgcgaact ggccgtggtg taccacctcc ataacctggt ggataatatc
      121 cgtctccggt tcaaagtgtt tactgatatc aatcagccgg acatttacac ggctaccggg
      181 ttgttctccg ctgctaactg gatggaaaga gaaacctatg atttcttcgg ggtcaacttt
      241 gtaggacatc cgaatctgaa gcgtatcctg aatgtagatg aaatggacta tttcccgtta
      301 aggaaagagt tcccgctgga agatcagaca aggattgata aggatgatga aatgtttgga
      361 agaggataat tttcaattag ccaataggcc aatgtgccaa ttggggagag actgaatgat
      421 aagaagtctt ttgaaattgg aggaataaat aagatagctg atcgtaagat tgaataacag
      481 acacatttaa aaaatgtcag aacatcatat caaattaccg gaagggtcga tcgagaaaac
      541 cactaccacg ctgaacctgg gtcctacgca cccggccaca cacggggtga tgcagaatat
      601 ccttgagctc gatggcgaaa ggatcgtttc caccgaacaa actatcggtt acatccaccg
      661 ggcttttgaa aagctggccg aacgtcgtcc gctggcacag attaccaccc tgaccgatcg
      721 tctcaattat tgctcttcgc ccctgaacaa tatgggctgg cacctgacct gcgagaaact
      781 gctcggggtg aaaacgccaa aacgcgtgga ctatctccgc gtgatcatca tggaactggc
      841 ccgtatctcc gatcacctga tctgtaattc tattgtgggt gtggacgccg gggcatatac
      901 cggtttcctg tatgtgatgc agtatcgtga actgatttat gaaatctatg aagaagtgtg
      961 tggcgcccgt ctcacaacca atatcggtcg tatcggtggt tttgaacgca acttcaacga
     1021 tatcgcattt cagaaactcg agaaatttct ccgcgaatat ccagccgtgc tgaaagaatt
     1081 tgaaaacctc ttccagcgta accgcatctt tatggaacgt accatcggta cgggtgccat
     1141 cagcgctgac agggcgttaa attatggatt taccggtccc aacctgcggg cagcaggcgt
     1201 ggattatgat gtacgggtgc attctccgta cagttcttac gaagattttc agtttaatat
     1261 acccgttggt actaccggcg acaactatga ccgctggctt gtacgtgaaa aagaaatgtg
     1321 ggagagtttg agcatcattg aacaggccta ccagaaagtt caggaattca aaggagctga
     1381 agccgatgtg tttcatgcgg atgttcccga gtactacctg cctcccaagg ctgaagtcta
     1441 taccaagatg gaagccctca tctatcattt caagatcatc atgggggaga ccaacatccc
     1501 ggccggggaa gtatataatt cgatagaagc aggaaacggt gaattagggt tttacctggt
     1561 tagtgatggc ggacgttcgc cctaccggct gcatttccgc cgcccctgtt ttgtatacta
     1621 ccaggcgtat gaagaactga tcaagggatc gatgctgagt gatgcgatca tgacgatgag
     1681 tagtttgaac ctgattgcgg gggagctgga cgcataggaa gtaaaaattc aaaagtaaaa
     1741 agtaaaaaat tctaaagcag agagtggatt agagatagca ccagcatgca aacagaattt
     1801 aaattttgaa attgatttaa cgggaataaa tggttttgaa ataacttaaa tagaaacagg
     1861 aaaagcagct gattgaatgt ttgtatcaga tctgtgagat ggattcagtt aaaggttttt
     1921 tactttttaa ttttgacttt tgatttttta tgataaagtt ctcagaaaat aaaatgaacg
     1981 aagtgcaggt catcatctcc cgctatcccc agggaaagca gaagagtgcc ctgatcccgg
     2041 tgctgcatat tgctcagcag gagtttggtg gctggctgag taccgaaacc atggactatg
     2101 tggcatctgt actccagctg aaaccgatcg aagtatatga agtggcgacc ttctacagta
     2161 tgttcaacct gaaaccggtg ggcaaataca tgtttgaagt atgccagacc ggtccctgta
     2221 tgctcaatgg cagtgatgat atcgtgaaat atatctttga caccctgggt ataaaacccg
     2281 gtgagacgag tgcagatgga ttgtttaccc tgaaaaccgt tgagtgcctg ggcgcctgtg
     2341 gctatgcgcc gatgatgcag cagggtaaat actaccggga acacctgacc aaagaaaaaa
     2401 ttgatgccat tattgcggaa tgcagaaaca cagccgcaca acaaaactga tgaaaaaaat
     2461 aatcaccata ttgtttttaa gcatgggcct ttccggcata gcccagtcaa aagaccaggt
     2521 gaaagtactg gccgcatcca aacagttcca ggaaaccgta ttcgggagca aggatagcct
     2581 gatactggaa cgattatttg ccgccactgt tcattatgag cattcaagtg gaaaagtgga
     2641 aaaccgggct gaagcgatcc gtggaatcag ccggaataaa tcagtctatg tgatgatacc
     2701 cgatccgtcg ccttacaaga tcagtaagca aggagataca cttgtggtga ataccgtgct
     2761 gaaagcggtg gagcagaaag ctgatggaag caaaacggac ctgaacctgt caatcgactt
     2821 gtactggata aaagaaggaa agcagtggaa gctgaccaaa agggtggcga caaaacggca
     2881 tgaatgaagt tctttgtaac gatagttctt ttatgctggc agatggccgg cctggcgcag
     2941 accgatgagg tcaaattgac cagtacactg aaagaatttc accaggccct ggtgaataag
     3001 aataaggtgt ccatcaacca gcaaactgat aaggcattga gctacggtca tagtaacggc
     3061 tgggtggaaa ccaagaaaga actgattgaa cacctggaaa gcggttatat ggagtataac
     3121 agtttccggg aggatagtat aaaagtggaa gtgaacggga atgtggcgca tgcccgtttt
     3181 atagcggaaa tcaatgccac gatgggcgga aaggaattca cgtttcagtt gcgggtgttg
     3241 gaagtgtggg tgaagaaagg gaaacggtgg gtgctgtttg caagacaggc cgtaaggaag
     3301 taaaaattaa aaagtcaaaa gtaaaaaaac ctatagcagg agtcggttta atgaatggtg
     3361 aatcgctgta atgtaaattc gaattgagtg ttttttaatt tttactttta acttttgact
     3421 tgttctggcc gattctttag aaagaagaaa ttataaaatg ggacgtaaat tattattaga
     3481 caaagcgcac gttgaaaaca tccggctcta cgatgtatac cgtcgtgaag gtggttaccg
     3541 cagtgtggag aaggccctga aatcaatgag ccccgaccag gtaacggagg aggtgaagaa
     3601 aagcggattg cggggacgtg g
//
LOCUS       DAOPMW010000356         3620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30655_length_3620_cov_8.644881, whole genome shotgun sequence.
ACCESSION   DAOPMW010000356 DAOPMW010000000
VERSION     DAOPMW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30655_length_3620_cov_8.644881"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1921)
                     /locus_tag="PLU11_13440"
     CDS             complement(2..1921)
                     /locus_tag="PLU11_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN60183.1"
                     /translation="MKQTLLLITAFFLIQSGFSQPYDPGKVNKKAQQYFDQAYERAAD
                     GNYANALGLLQLAVKTDPRFVDAYLAMGNLYGKMKNYKTSIENYEKAFAIDSGYTLPE
                     RLPYSVNLAGIGEFEKALAAVNELLEKKKPSNPITLENLGKRKLSYEFAVEYAKNNPE
                     KNYVFAPMNMGDSINSNESEYFPCLTVDGKEFTFTRKLNGINEDFFYSELYKDSSWSR
                     SKPMEGNVNTENSEGAQNISQDGQWLVFTGCYRKDGMGGCDLYISYKTEKGWSEAINL
                     GPGINTDQWDAQPSLSPDKRELYFVSRRFGGFGGSDIYVCRMQPNGRWGKPENLGGGI
                     NTPGDEQCPFIHADNQTLYFTSSFWQGYGDEDLFFVRRQPNGSWTRPVNLGYPINTID
                     REGTLFIASDGKTAYYASDRNDSRGGLDIYRFELRENVRPFKTLWVKGKVFDKKTNAG
                     LPSTVELIDLETKKPMTQVETNEIGEYLITLAIGKDYAFNINKKGYLFYSDNFSLKGI
                     NPDTTYEKNIGLQPFEVNANIVLNNIFFDVNKFELKTESQAELDLLVQLLLDNPTVKI
                     EIGGHTDNVGKPADNLALSNNRAKAVVNYLVSKNIPAQRLTAKGYGETKPVADNKTEA
                     GRAINRRTELKVIGK"
     gene            complement(2288..2908)
                     /locus_tag="PLU11_13445"
     CDS             complement(2288..2908)
                     /locus_tag="PLU11_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPN60184.1"
                     /translation="MNEVKNDMVALPVMEHFYTLQGEGFHQGRAAYFIRLGGCDVGCV
                     WCDVKDSWDADKHPKFSVEQLVTAVKKTPTQLVVITGGEPLMHQLDELTIALQAAGLE
                     TNIETSGAHPLSGHWNWICLSPKKFKEPLPGILPHAHELKVVIFNKSDFDWAEKYAAL
                     VSPSCKLYLQPEWDKAAQVTPLIIDYIKSHPQWELSLQVHKYINVP"
     gene            complement(2920..>3620)
                     /locus_tag="PLU11_13450"
     CDS             complement(2920..>3620)
                     /locus_tag="PLU11_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPN60185.1"
                     /translation="GFKSTLIHYEETISETKLLDKIRDLNTDPDIDGILVQLPLPKHI
                     SDEEVINSIDPDKDVDGFHPINVGYMVQGLPTFVPATPHGIMLLLEHFKVETKGKHAV
                     VVGRSNIVGRPMSILLSGNTNPGNCTVTICHSQTKNIKEFCLQADIIVAALGKPDFIT
                     ADMVKEGAIVIDVGITRVEDATKKSGFKLKGDVDYENVAPKCSWITPVPGGVGPMTIA
                     ALMKNTYRSCSQKN"
BASE COUNT          893 a          856 c          847 g         1024 t
ORIGIN      
        1 attatttacc gatcaccttt agctcggttc ttcggttaat tgcccgtccg gcttctgttt
       61 tattatcagc cacgggtttg gtttccccat agcctttggc ggtcagccgt tgggccggaa
      121 tattcttgct gaccaggtaa ttcaccaccg ctttagcacg gttattactc agggccaggt
      181 tgtctgccgg ttttccgaca ttatcggtat ggccaccgat ctcaattttc accgtgggat
      241 tatccagcag caactgtacc agcaggtcca gttcggcctg ggattcggtt ttcaattcaa
      301 acttgttgac atcaaagaaa atattgttca ggacaatatt cgcattgact tcaaaaggct
      361 gcagcccgat atttttttca taggtggtat cggggttaat gcccttcagg gaaaaattat
      421 ccgagtaaaa caggtatcct tttttattga tattgaacgc atagtctttc cctatggcta
      481 aggtgatcag gtattcgcct atttcattgg tttccacctg ggtcatgggt ttcttggttt
      541 caagatcaat cagctcaaca gtggagggga gaccggcatt tgttttttta tcaaatactt
      601 ttcccttcac ccatagtgtt ttaaatggcc ggacattttc cctcaattca aaacgatata
      661 tatcgagccc acccctggaa tcattccggt cactggcata atatgccgtc ttgccatcag
      721 aagcaataaa caaagtgcct tcgcggtcaa tggtattaat cgggtatccg aggttgacgg
      781 ggcgtgtcca gctgccatta ggctggcggc ggacaaaaaa cagatcctca tcgccgtagc
      841 cctgccagaa ggaggaagtg aaatacaaag tctgattatc ggcatggata aagggacatt
      901 gttcatcccc gggtgtattg atgccacccc cgagattttc aggtttaccc cagcgaccat
      961 taggctgcat gcggcagaca taaatatcgc ttccgccaaa tccgccgaag cgacggctta
     1021 caaaatagag ttcgcgttta tcaggtgaaa gactcggttg tgcatcccat tggtcggtat
     1081 tgataccagg cccaagattg atcgcttcgc tccagccttt ttctgtttta taggaaatat
     1141 acaggtcgca accacccatg ccgtccttgc ggtagcaacc ggtaaacacc agccattgtc
     1201 catcctgtga aatattctgg gctccttcac tgttctccgt attcacattg ccctccatgg
     1261 gtttggagcg gctccagctg ctgtctttgt atagctcgct gtaaaagaaa tcttcattga
     1321 ttccatttag tttacgggta aacgtgaact cctttccatc aacggtgagg cagggaaaat
     1381 attcggattc attggagttg atactgtcgc ccatattcat gggagcgaaa acataatttt
     1441 tctccggatt attctttgca tattccacgg caaactcata agaaagtttt cgtttgccca
     1501 ggttttccag ggtgatgggg ttactgggtt tctttttttc caggagttca ttcacagcgg
     1561 ccagggcttt ttcaaattcg ccgattccgg ccaggtttac actgtagggc aatcgttccg
     1621 gcagggtata gccggagtcg atggcaaagg ctttttcgta attttcgata ctggttttgt
     1681 aattcttcat cttaccataa agattcccca tggcgaggta ggcatccaca aagcggggat
     1741 cggtcttaac ggccagttgc aggagtccga gggcattggc ataattgcca tccgccgccc
     1801 tttcgtaagc ctggtcgaaa tattgctggg cttttttatt tactttcccc gggtcgtagg
     1861 gctgggaaaa tccggactgg attaaaaaga aagcggtaat taaaagtagc gtttgcttca
     1921 tgcagggaaa ttgggatttc tataacgaag ctaagctaaa attattcctc tttttagcgg
     1981 aaaatgttaa agaaaggagt gcttcgacaa gctcagcatg acaagtgcgc cattgtcacc
     2041 ctgagtagcc gtaaccaaaa agcacagggt tgctgaaaaa tgttcaccgg cgtaccgaag
     2101 ggcggcaagc ctgcctaccg gacaggcagg ctcaccatga caaaactaca tccacggcat
     2161 ggcagaaatg ctcgttgtca ccctgagcgt gtcgaatggt gtataagtaa tacttcgtca
     2221 gtgcttcggc aagctcagca tgacaacaac agctcaacaa aacaagatat ctcgcataac
     2281 gtaagaatca gggcacatta atatacttat gcacctgcaa actcagttcc cattggggat
     2341 gggactttat ataatcgatg atcaggggag taacctgtgc ggctttatcc cactcgggtt
     2401 gcaggtatag tttgcaggat ggggagacca gtgccgcgta tttctcggcc cagtcgaaat
     2461 ctgatttatt aaagatcacc actttcagtt catgtgcatg tggtaaaata ccgggcaacg
     2521 gttccttgaa ttttttcggt gagagacata tccagttcca gtgaccactg agcggatgag
     2581 caccggaggt ttcaatattg gtttcaaggc cggcggcctg aagggctatg gttaattcat
     2641 ccagttgatg catcaggggt tcaccaccag tgatcactac gagttgggtg ggcgtttttt
     2701 ttacggctgt cactaattgt tcaacggaga acttcggatg tttatccgcg tcccagctgt
     2761 cttttacatc gcaccacacg cagcctacat cacaacctcc taaccggata aaataggcgg
     2821 ctcttccctg gtgaaagcct tcgccctgca gggtataaaa atgttccatt accggaagcg
     2881 ccaccatgtc attcttcact tcattcattt taaaaaaact taatttttct gactgcagga
     2941 acggtacgtg ttcttcatca gggcagcaat ggtcatgggg cctacaccac ctggtacggg
     3001 ggtgatccag gaacatttcg gcgctacatt ttcataatcc acatcgcctt ttaatttaaa
     3061 gccacttttt tttgtggcat cttccacacg ggtgatgccc acatcaatca caatcgcgcc
     3121 ttctttaacc atatccgcag taataaaatc cggtttgccc agcgcggcca caatgatatc
     3181 tgcctggaga cagaattcct taatgttttt ggtctgcgaa tgacaaatag tgacggtgca
     3241 gttaccaggg ttggtattgc cgcttaacaa aatgctcatc gggcggccta ctatattgct
     3301 gcggccgacg accacggcat gcttcccttt ggtttctact ttgaaatgtt cgagtaataa
     3361 cataatgcca tgcggggtag ccggtacaaa agtgggcaga ccctgtacca tgtacccgac
     3421 attgatcggg tgaaagccat ccacatcttt atcgggatcg atactgttga tcacttcttc
     3481 gtcagaaata tgtttgggca ggggcagctg caccaggatg ccatcaatat ccggatcggt
     3541 gttcagatca cggattttat ccagcagttt ggtttcactg atcgtttctt cataatggat
     3601 cagggtggat ttgaaaccca
//
LOCUS       DAOPMW010000357         3612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30751_length_3612_cov_6.165870, whole genome shotgun sequence.
ACCESSION   DAOPMW010000357 DAOPMW010000000
VERSION     DAOPMW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30751_length_3612_cov_6.165870"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLU11_13455"
     CDS             complement(<1..487)
                     /locus_tag="PLU11_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN60186.1"
                     /translation="MEEPIFQQRTDLSALNAAVMAIRSEIKKIIVGQDEMVKLIIAAL
                     LADGHVLIEGVPGVAKTLTAKLVAKSVNAGFSRVQFTPDLMPSDVLGTPVFNPKEASF
                     EFKKGPVFSNIILVDEINRAPAKTQSALLEIMEERQATIDGKTYPMASPFMVLATQNP
                     VE"
     gene            complement(490..1716)
                     /locus_tag="PLU11_13460"
     CDS             complement(490..1716)
                     /locus_tag="PLU11_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60187.1"
                     /translation="MLVTVLAAAIILLYISGKAARKRNFDDRVTLKRQDKIPYATYVA
                     FQQLPYQFPGARIQVTRKEPGYWDSVSVYDDRQAFICITDRFGADESEMKKLIDFARY
                     GNDVFISARYISSKADEFLRCGSNAPEYYRQYPGDEEDGKVRLTLNTPPFSDTVHYQY
                     PGKTFSSYFTQVDERSTQVLGFDESGRPDFIRLQTGKGNFYVHLEPLAFSNYFLLHEK
                     NIGYYEKVFSLISPDVKSIIWDEYYLNKKMSYDQSQQQKGWMTVLMNMKNAEGEKPFR
                     AAFWLLILLLIVYVLMEMRRRQRYIPVVRKPRNDSLDFVKTIGRLYYDRSDHKNLCRK
                     MSAYFLEFVRSNYKLPTGILNDDFIRLLHFKSGVEEKEIAEIVSFIRQLDEGDTIHER
                     QLAFFHNRLESFYKKA"
     gene            complement(1743..2585)
                     /locus_tag="PLU11_13465"
     CDS             complement(1743..2585)
                     /locus_tag="PLU11_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPN60188.1"
                     /translation="MSRNTLNPISFSLVAWKRFLVLLIMALLSGGLLQAQTDDPDMEE
                     EDTTITRTTIEVAPVETLDEEEESTAHFIPLRDMDSVSLYRRKAPDSVINALQEDDDF
                     WYANANIEKKKPEKEKRRRNLQPDWGFSWMSTILWVVIVGVFLAVVIYYMAESGLFRR
                     KRRVVTDNRKDPSLEEMPEDIFAIRYQQEIDKAVAGGDYRLAVRLQYLRLLKHMAEKN
                     RIRYQQDKTNMDYLLQLYNTEYYHDFFRITRHYEYSWYGRFAVSPDAYRLISQDMEQF
                     QNKR"
     gene            complement(2585..3553)
                     /locus_tag="PLU11_13470"
     CDS             complement(2585..3553)
                     /locus_tag="PLU11_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPN60189.1"
                     /translation="MREALFIKKNKDRWLKNQHLPPDDPDEMAASFTQLVDDLAYAKT
                     FYPSGKVTHYINSQASRIYLDIYKNRKEDSNRIWKFWKYDLPLTIRKHHGILLFATLF
                     FILFFVIGFFTASVDESVAAEVLGPGYIEETHKNIEKGNPFGIYEGGNSILTWLGIMV
                     NNMIVALRMFASGLALGIPSLYGLAETGVMVGVFDQIFAAKGLAADFWLVVFVHGTLE
                     ITAIILAGGAGLILGKGFLFPGTIKRMDAFKQSAKDGTKIMIGLLPVFAVAAFFEGFI
                     TRLYNDLSWLTTGVFSVSVIFVIWYFIIYPIRLHKRLSVQLNEEDV"
BASE COUNT          975 a          878 c          786 g          973 t
ORIGIN      
        1 gttctaccgg gttctgcgta gccagtacca tgaacggaga ggccatcggg taggttttgc
       61 catcgatggt tgcctgtcgt tcttccatga tttccagcaa ggccgactgt gttttagccg
      121 gagccctgtt gatctcatcc accagtatta tattgctgaa tactggtcct tttttaaatt
      181 caaaactggc ttccttggga ttgaatacag gcgtacccaa tacatcggac ggcatgagat
      241 cgggggtgaa ctggacccgg gagaagccgg cattcacgct ttttgccacg agttttgcgg
      301 taagggtctt ggccacaccg ggcacacctt cgatcagtac gtgaccgtct gccaacagag
      361 cggctataat caacttcacc atttcatcct gacctacgat aattttttta atctcggaac
      421 ggattgccat aactgctgca ttcagtgcag agagatcggt acgttgttga aaaattggtt
      481 cttccatatt tatgcttttt tatagaatga ttcaagtcgg ttatgaaaaa aggcgagttg
      541 ccgttcatgt atagtatccc cttcatccag ttgccggata aaggaaacga tttcagcaat
      601 ctccttttct tcaacccctg acttaaagtg gagcagccgg atgaaatcgt cattgagaat
      661 acccgtaggc agtttataat tgcttcgtac aaattcaagg aaataggcac tcattttccg
      721 gcatagattc ttatggtcac tccggtcata atacagccgg ccaatcgttt tcacaaaatc
      781 gagtgagtca ttgcgcggtt tccgtaccac cggtatataa cgctgccggc gccgcatttc
      841 catcagcaca taaacaatga gcaacaggat taacaaccag aatgcggccc gaaatggctt
      901 ttccccttct gcattcttca tattcatcag gaccgtcatc caccccttct gctgctgcga
      961 ctgatcgtac gacatttttt tattgagata atactcatcc caaataatgg atttcacgtc
     1021 cgggctgatc agggaaaaaa ctttttcata atacccgata tttttttcat gcagtaaaaa
     1081 ataattggaa aaagccagcg gttccaggtg aacgtaaaaa ttacctttcc ccgtctgcag
     1141 gcggataaaa tccggacgtc cgctttcatc aaaaccgagc acctgggtag aacgttcatc
     1201 cacttgtgtg aaataagatg aaaaggtttt accagggtat tgataatgta cggtatcaga
     1261 aaatggcggg gtattaaggg tcaaacggac tttcccgtcc tcttcatcac ctggatactg
     1321 tctgtaatac tccggagcgt tactgccaca acgaagaaat tcatctgctt tggaagagat
     1381 atagcgggca ctgataaaaa catcatttcc gtaacgtgca aaatctatca gcttcttcat
     1441 ttctgattcg tctgccccga accggtccgt aatgcagata aaagcctgcc ggtcatcata
     1501 tacggaaacc gaatcccaat accctggttc tttccgggtt acctgaatac gggcaccagg
     1561 aaactgataa ggtaactgct gaaaagctac ataagtggca tagggaattt tatcctgccg
     1621 ctttaatgta acacggtcgt caaagtttct tttcctggcc gcctttcctg aaatatataa
     1681 caggatgatg gcggcagcca gcacagtaac aagcaaatat ggtagtatac gtttcacctg
     1741 ggttatcgct tgttttgaaa ctgttccata tcctgcgaaa tcagccggta ggcgtccggc
     1801 gatacagcaa accggccata ccagctatac tcgtaatggc gggtgatacg gaaaaagtca
     1861 tgataatatt cagtattata cagctgcaac aggtagtcca tattggtttt gtcctgctga
     1921 tagcggatcc ggtttttctc tgccatatgt ttcaacaacc ggagatattg caggcgtacg
     1981 gcaagccggt agtctccacc tgcaacagcc ttatcaattt cctgctgata gcggattgca
     2041 aatatatctt ccggcatttc ctccagtgat gggtcctttc tattatccgt caccactctc
     2101 cttttgcgtc tgaacaaacc actttcagcc atatagtaaa tcaccaccgc gagaaatacg
     2161 ccgacaatca ccacccaaag aatggtcgac atccagctaa agccccagtc tggttggaga
     2221 ttccggcgcc tcttttcttt ttccggtttc tttttttcta tgttggcatt ggcataccag
     2281 aagtcgtcgt cctcctgtaa agcgttgatc acactgtcgg gggctttccg gcggtaaagg
     2341 ctgactgaat ccatgtcacg gagtggaata aaatgagcag tggattcttc ctcttcatct
     2401 agggtctcaa ccggcgccac ttctattgtc gtccgggtga tggtggtatc ttcctcctcc
     2461 atgtcaggat catccgtttg cgcctgcaat aaaccacctg aaagcagggc catgatcagc
     2521 aagaccagaa agcgcttcca ggccacaagg gaaaaagaaa tcgggttcag ggtattccgg
     2581 ctcactatac gtcctcctcg tttaactgta ccgataagcg cttatgcagt cggatggggt
     2641 aaatgataaa ataccagatg acaaaaatga cagaaacaga aaacacaccg gttgtcagcc
     2701 aggaaagatc gttatacaga cgtgttataa atccttcaaa aaatgcagcc acagcaaata
     2761 caggcaacag gccgatcatg atcttagtcc cgtcctttgc actttgtttg aacgcatcca
     2821 tccgtttgat tgttccggga aaaagaaatc cctttcccag gatcagtccg gccccaccgg
     2881 ctaatataat tgctgtaatt tcaagtgtac cgtgtacaaa aaccaccagc cagaaatctg
     2941 cagccagtcc tttcgccgca aatatctggt caaacacgcc gaccatcacc ccggtttcag
     3001 ccaacccata aagggaaggt attccaagtg ccagtccaga agcaaacatg cgcagggcga
     3061 cgatcatatt atttaccatg attcccagcc aggtcaggat actgtttcct ccttcataga
     3121 tgccaaaggg gtttcctttt tctatatttt tatgtgtttc ctcgatatag ccggggccca
     3181 gtacttccgc ggcaacagat tcatccacgg aagccgtaaa aaagccaatc acaaaaaaaa
     3241 ggataaaaaa gagggtggca aaaagcagta tgccatgatg tttgcggatg gtcagtggca
     3301 gatcatactt ccagaatttc cagatccggt tggaatcctc tttcctgttt ttatagatat
     3361 cgaggtatat ccgcgaagcc tgcgaattga tatagtgggt aaccttccca gatggataga
     3421 atgttttggc atacgccaga tcatccacca gctgtgtaaa ggaagcggcc atctcatccg
     3481 ggtcatccgg gggaaggtgc tggtttttca gccagcggtc tttattcttc tttataaaca
     3541 gggcttccct caatcgatgt attttgctcg taaaatacta tttttaaaat aagaaaagca
     3601 ggacctgccg aa
//
LOCUS       DAOPMW010000358         3599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30916_length_3599_cov_5.186795, whole genome shotgun sequence.
ACCESSION   DAOPMW010000358 DAOPMW010000000
VERSION     DAOPMW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30916_length_3599_cov_5.186795"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLU11_13475"
     CDS             <1..813
                     /locus_tag="PLU11_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60190.1"
                     /translation="LAVDNHCISTVMLGGKEYFLDGTEKYVPFGEYAWRIQGKEVLIG
                     KGDKYEVKKVPVQDKEKNKLLTQTSFQLKDNLLKGHVKVTLTGEQRTGFHQFYHDLPN
                     DDKKKMIQRYLEFGNRNLVVSNVKTSDLNNREIPIVFEGDIDLSNYVITEDKELYLGI
                     DFFPENLRGLQPEKDRQQDYALSSSYVSVDETELILPAGYKVASLPAPISEKTTDYEV
                     TGSYTAKDNKVIFSKTLAFNTGRIRKADFENWKSFTKKLKDFNSNLILVKKP"
     gene            1066..3210
                     /locus_tag="PLU11_13480"
     CDS             1066..3210
                     /locus_tag="PLU11_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60191.1"
                     /translation="MKKVLLFLNLLLSFTLVAQDDDTFSPSKLEAIARNMKTVWDDTD
                     PDFGISSVPDKWKEESGVIIAQKTRFSFDKDANKLAVYEITRRRIKLNDRDAVNSYSS
                     VYFRIGSANDGAGIKVIKANGSVQDVSLRNAVYVEDNDDVPSTFTPYIGKASTYLDKS
                     KSRVIFFKIAVPDLDPGDIIDYGTIFYDDNTVKKMSYIEFDPIYFVCTREYPVLSQKF
                     EIDTDNNSFVNSKSTMGAPAFKETGNANAEYSWEDRNREKIPDTKWVNRMIEFPMLKF
                     QIVFSRSENRSDLFIGDRGELKQTISPDELAKKMNHLYNRLDGSMYYSMAKAYLKQIG
                     YADLREEDFIQKTYYILRHMSFYRANGFSSELFASCLTQCLELKSIPYELVVTAPLTL
                     TKPGDIIFRTEPEWMVKVKDKFIFNATIFSNPYDFKEEFLNTPAYIISLGKTPTATPL
                     TLPATKADDNITTNTIIASMDTATRNMKVQLQRAATGLAKKKYNYQGLVYTTAFDDDH
                     RSYGGEDDVRASMKGAALDSYEEKLRERKKEDKTRKLETMKNELDDDYDNLTAYTEFT
                     LNSDGRTWRKQELNYTNKFELGDMVKIAGDNLLVAVPGLIADQLWISQDDRKREVDAY
                     MQYPESIRNIINFTIPAGYKVVGIQSLNTNIDNAVGTFAVQASVEGNTLNILVKKHYK
                     QTTVKKEDWSKLLEMLDAAYNFSQKKVLLKKL"
     gene            3311..>3599
                     /locus_tag="PLU11_13485"
     CDS             3311..>3599
                     /locus_tag="PLU11_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60192.1"
                     /translation="MNPCPVADDGDPAKRLTKTISSIAFLVIILVFTACADKAEQKPL
                     PSPGQDSGYLKEKEKKRVDSLAALTRKKKKIYLTFDDGPNKGTRNVLEVIKT"
BASE COUNT         1099 a          826 c          812 g          862 t
ORIGIN      
        1 ctggcggtgg acaatcactg tatcagtaca gttatgcttg gaggcaagga atatttcctg
       61 gatggtacgg aaaagtatgt accctttggc gaatatgcct ggcggatcca gggcaaggag
      121 gtcctgatcg gcaagggcga caagtatgaa gtaaaaaaag ttcccgttca ggataaggag
      181 aaaaataaat tactgaccca gaccagtttc cagttaaaag ataacctgct caaagggcat
      241 gtgaaagtca ccctcaccgg ggagcagcgg accggtttcc accaattcta tcatgatctg
      301 cccaatgatg ataaaaagaa aatgatccag cgctatctcg aatttggcaa ccgcaacctg
      361 gtcgtgtcaa atgtcaaaac atcggacctg aacaaccgcg aaataccaat tgtatttgaa
      421 ggcgatattg atctcagtaa ttatgtgatt accgaagaca aggaacttta cctgggtatt
      481 gactttttcc cggaaaatct gcggggactg caaccggaaa aagaccggca acaggactat
      541 gccctctcct ccagctatgt atccgtggat gaaaccgaac tgatcctgcc agccgggtac
      601 aaagtggctt cgttacccgc accgatcagc gagaaaacaa cagactatga agtcacgggt
      661 tcgtataccg ccaaagacaa taaagtcatt ttcagcaaga cactggcctt caataccggc
      721 aggatacgga aagcggattt tgagaactgg aagagtttta cgaaaaagct gaaggatttt
      781 aacagcaacc tgattttggt gaaaaaacct tgaggaagtg ggcattcggc agttggcagt
      841 tggcagttgg cagaacttac taaataaatt atttgaataa cagtgataat agcaattaag
      901 aaaaaatgag ataaagattt gagtgtggca aaacggagat tacgaaaaga gtcagaattt
      961 tcctccttta ctcattactc attactgatt actcattcat aacctgaaca gcaaagaaaa
     1021 aatatcaggc tctttcatca taagtcgcct gcaaagaaat taagtatgaa aaaagtactc
     1081 ttatttttaa acctgcttct ctcattcacc ctggttgcgc aggacgacga cactttcagc
     1141 ccctccaaac tggaagccat tgcccggaat atgaaaacag tatgggatga taccgatccg
     1201 gattttggta tcagttcagt tcctgataaa tggaaagagg aaagcggggt gatcattgcc
     1261 cagaaaaccc ggttttcctt tgataaggat gccaataaac tcgcggtgta tgaaatcacc
     1321 cgccgccgga tcaaattgaa tgaccgggat gccgtgaata gttattcatc tgtttacttc
     1381 cggattggtt ccgccaatga cggagccggt ataaaagtaa tcaaggccaa tggttcggta
     1441 caggatgttt ctctccgcaa tgcggtgtat gtggaagaca atgatgatgt gccctccaca
     1501 tttacgccct atatcggcaa ggccagtact tacctggaca agagtaagag ccgcgtcatc
     1561 ttctttaaaa ttgccgttcc tgatctggac ccgggtgata tcattgatta tggcaccatt
     1621 ttttatgatg ataacacggt gaaaaagatg agctacatcg agtttgaccc gatatatttt
     1681 gtttgtaccc gggaataccc ggtgctcagc cagaaatttg aaattgatac cgataacaat
     1741 tcctttgtca actccaaatc caccatgggt gctcccgctt ttaaagaaac agggaatgcc
     1801 aatgctgaat acagctggga agaccggaac cgggaaaaga tcccggatac caaatgggtg
     1861 aaccggatga tcgaattccc catgttgaaa ttccagatcg tcttctcccg cagtgaaaac
     1921 cgttctgatc tttttatcgg agaccggggg gaactgaaac aaacaatcag cccggatgaa
     1981 ctggcaaaaa aaatgaacca cctctacaac cggctcgatg gttccatgta ttactccatg
     2041 gccaaagcct atctgaaaca aatcggctat gcagatttgc gggaggagga ttttatccag
     2101 aaaacctatt atatcctacg ccacatgtct ttttaccggg ccaatggttt ttccagtgag
     2161 ctttttgcct cctgcctcac ccaatgcctg gagttaaaga gtattcccta cgaactggtg
     2221 gtgaccgctc ccctgacatt gaccaaaccc ggtgatatca ttttccgcac tgaaccggag
     2281 tggatggtga aagtgaaaga caaatttatt ttcaatgcta cgattttttc caacccctac
     2341 gatttcaaag aagaattcct gaatacccct gcttacatta tcagtctggg taaaacacca
     2401 acggctactc cactcacatt acccgctacc aaagcggatg ataacatcac gaccaatacg
     2461 atcattgctt ctatggacac tgccacccgg aatatgaagg tacagttgca gcgggcggca
     2521 acaggattgg ccaagaaaaa atacaactac cagggactgg tgtataccac cgcatttgat
     2581 gatgaccatc gcagttatgg cggcgaagat gatgttcggg ccagtatgaa aggagctgca
     2641 ctggatagtt atgaagaaaa actccgggag cggaaaaaag aggacaagac ccgcaagctg
     2701 gaaaccatga agaatgaact ggatgacgat tatgataacc ttaccgccta tactgaattt
     2761 accctgaact ccgatggtcg tacctggcgc aagcaggagt tgaattatac caataagttt
     2821 gaactgggtg atatggtaaa aattgcgggg gacaatctgc tcgtggctgt acccgggctg
     2881 atcgcagacc agttatggat ttcgcaggat gaccgcaaac gggaagtgga tgcctatatg
     2941 caatacccgg aatccatccg gaatatcatc aatttcacca tccccgccgg ttacaaagta
     3001 gtcggcatcc agagcctgaa taccaatatt gacaatgccg ttggcacttt tgccgtacag
     3061 gccagcgtgg aaggtaacac cctcaatatc ctggtcaaga aacattacaa gcaaaccact
     3121 gtaaaaaagg aggattggtc caaattgctg gagatgctgg atgccgctta taatttttcg
     3181 cagaagaaag tattattgaa gaagctgtaa gaaagcgggg actttaatca ttctttaacc
     3241 aaacgccatt tgcaatcctg ctgccaactc catactttca tggtggaaat taaaacgcca
     3301 cacccttttt atgaacccct gtcctgtcgc cgatgatggc gaccctgcca aacggctgac
     3361 caaaaccata tcttccattg ctttcctggt aataattctt gtttttactg cctgtgctga
     3421 taaagcagag caaaaacccc tgccctcacc gggccaggac tccggatacc tgaaggaaaa
     3481 ggagaaaaaa cgcgttgact ccctggcagc ccttacccgg aaaaagaaaa aaatctatct
     3541 cacttttgat gacggcccca acaaaggcac ccgcaatgtg ctggaagtga tcaaaaccg
//
LOCUS       DAOPMW010000359         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30925_length_3598_cov_7.800169, whole genome shotgun sequence.
ACCESSION   DAOPMW010000359 DAOPMW010000000
VERSION     DAOPMW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30925_length_3598_cov_7.800169"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLU11_13490"
     CDS             complement(<1..1188)
                     /locus_tag="PLU11_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPN60193.1"
                     /translation="MQYENAYNFLLPKLQHELPSWVTYHTAAHTRSVLNSAITLAKAE
                     LVEGEDLILIKTAALFHDAGFLHQSDGHEELSCRIAEEHLPRFGYSPEQIARVCRIIM
                     ATRIPQAPVDHLGRIMADADLFYFGTDEYDQTSAKLLEELKQSGKQKTASEWLLMQTE
                     FLTAHQYFTESAKKMLDEKKADNLRKINARLESSIQAHHESSPRQLVLDSMLAFAGVM
                     IAAIALKLFLVPNHFFDGGITGISLLVHEIYDFNLGLVILLFNLPLIIVSYYSIGQRF
                     ALRTLMAVILLGICLMLIPMYALTADKLLICIFGGAFLGIGIGLIMRTGSALDGIEVL
                     AVYTLKRTSFTITEIILGINIVIFTIAGFRFGIETALYSILTYFTATRCIDYVVEGLQ
                     AYTG"
     gene            complement(1214..2254)
                     /locus_tag="PLU11_13495"
     CDS             complement(1214..2254)
                     /locus_tag="PLU11_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPN60194.1"
                     /translation="MAKILVVDDEADLEVLIRQKFRQKIRENLYQFVFAANGVHALEQ
                     LTQHHDVDVVLSDINMPEMDGLTLLTKLGEQNGLLKSVIVSAYGDMDNIRTAMNRGAF
                     DFITKPVNFEDLELTMEKTIKHVTQLRETMKAIKENNILRMYVDETVLNFMGGREFES
                     SLAANETVEATIAFFDICGFTSISENETPDKVVQLLNSYFDVMVKEIIAQGGYIDKFM
                     GDAVMAVFRGDYHLDRAIDAALAVRTKIANLPVVQELKSYTPKVSTGINSGEMISGNI
                     GSATLRRLDYTVIGDVVNTAQRLQSSAAPGQILISESSYQKVKDSFNCNSVGEMHLKN
                     KSQPVNTYEVVD"
     gene            complement(2254..2631)
                     /locus_tag="PLU11_13500"
     CDS             complement(2254..2631)
                     /locus_tag="PLU11_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN60195.1"
                     /translation="MMKILVVDDETDVQTLFEQRFRREIKNGEMHFDFAFSGEQALKY
                     LNEHEQEAVLILSDINMPGMSGLELLGQIKSKYFKPPPVVMMITAYGDAENFNTAKQL
                     GADDFLTKPVDFTELKEKLKTLI"
     gene            complement(2636..>3598)
                     /locus_tag="PLU11_13505"
     CDS             complement(2636..>3598)
                     /locus_tag="PLU11_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60196.1"
                     /translation="VLKKTGDHWLIVHVHGSMPDYRLAGQNYMTNAETSKRNIELEQQ
                     VFQRTKELNKTIEILKSTQAQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNTE
                     LIDEMKEEFSKGNTTEAVQLAEDIKQNNEKISFHGKRADSIVKGMLQHSRNSSGQKEL
                     TDINNLADECLRLSYHGFRAKDHSFNSKTETHFEEGLPDIQVAPQDIGRVLLNLITNS
                     FYSVAQKRKQVGEGYEPMIEVATKNLGNAIEIRVRDNGNGVPQKVLDKIFQPFFTTKP
                     TGEGTGLGLSMSYEIITKGHGGQLQVDTQEGEYAEFRITIPFKH"
BASE COUNT          899 a          898 c          778 g         1023 t
ORIGIN      
        1 accggtatag gcctggagac cttccaccac ataatcaata caacgggtgg cggtgaaata
       61 cgtaagaata gaatacaaag ctgtttcaat accaaagcgg aagccggcaa tggtgaagat
      121 gacgatattg atccccagga taatctccgt gatcgtaaag gaagttcgct tcaatgtata
      181 gaccgccagt acttcaatgc catccagtgc ggaacctgtc cgcataatca gaccaattcc
      241 aattcccagg aaagcaccgc cgaagataca gatcagtagt ttatcggctg tcaatgcata
      301 cataggaatc aacatgaggc aaatgcccaa gaggattacg gccatgagtg ttcgtaaagc
      361 gaaacgctgt ccgatggaat aataactgac gatgatcagt ggaagattga ataacaaaat
      421 caccagtccg agattgaaat cataaatctc atggaccagc agggagattc ccgtgatccc
      481 gccatcaaaa aagtgattgg gcaccaggaa taatttcaag gcaatggccg caatcatcac
      541 ccccgcaaag gccagcatac tgtccagtac cagctgccgc ggactgcttt catgatgtgc
      601 ctgtatgctg gattcgagcc gggcattgat ctttctcagg ttatccgcct tcttttcatc
      661 cagcattttt ttggcacttt ccgtaaagta ctggtgggcc gtgagaaatt cagtctgcat
      721 cagtaaccac tccgaagcgg ttttttgttt accagactgt ttaagctctt ccagcaattt
      781 ggcagaagtc tgatcatatt catctgtacc aaaataaaaa agatccgcat ccgccatgat
      841 ccggccaaga tgatccaccg gtgcctgggg aatacgggtg gccataatga tccggcaaac
      901 cctggcgatc tgttcggggc tgtatccgaa acggggcagg tgttcttctg caatacgaca
      961 ggataattcc tcatgcccat ccgactgatg aagaaaaccg gcatcgtgaa acagagcggc
     1021 cgttttgatg aggatcagat cctctccctc caccagctca gctttagcca gtgtaatggc
     1081 tgaattcagc accgaccggg tatgcgcagc cgtatgatag gtcacccagg aaggaagctc
     1141 atgctgaagc ttgggtagca ggaaattata ggcgttttcg tattgcatgt tcttacaagt
     1201 taaaataaat attctaatcc actacttcat aggtgtttac cggctgcgat ttgtttttca
     1261 agtgcatctc ccccactgag ttgcaattaa atgagtcttt tactttctgg taggaggact
     1321 cgctgatcag aatctggcct ggtgccgcgg aagactggag tcgctgagcg gtattcacca
     1381 catccccgat caccgtataa tccagccggc gcagggttgc cgaaccaata ttcccggaga
     1441 tcatttcacc ggaattgata ccggttgaca ctttgggcgt ataacttttc agttcctgca
     1501 caaccggaag attggcaatt tttgtccgga ccgccagtgc cgcatcgatg gccctgtcca
     1561 ggtggtaatc accccggaac accgccatca ccgcatcacc cataaactta tcaatataac
     1621 caccctgtgc aatgatttct ttgaccatca catcaaaata actgttgagg agttgcacca
     1681 ctttatcggg tgtctcattc tcgctgatag aggtaaagcc acagatatca aaaaaagcaa
     1741 tcgtggcttc caccgtttcg ttagccgcca aagaagattc aaactcacgt ccgcccataa
     1801 agttcagcac cgtttcatcc acatacattc tcaaaatatt gttctccttg atggctttca
     1861 tggtttcccg caactgcgtg acatgtttaa tggtcttctc catcgtcagt tcgaggtcct
     1921 cgaaattgac tggcttggta ataaaatcaa aagctccccg gttcattgcg gtacggatat
     1981 tatccatatc gccgtaagcg gaaacgatca ctgatttcaa caagccattt tgttcaccca
     2041 gtttggtgag cagggttagt ccgtccattt caggcatatt gatatcgctg agcaccacat
     2101 ccacatcgtg gtgttgggtc agctgttcca gcgcatgcac cccgtttgcc gcaaatacaa
     2161 actgatacag gttctcccgg atcttctgcc ggaacttctg ccggatcagt acttccaggt
     2221 ctgcttcgtc gtctactaca agtattttag ccatcagatc agtgttttta atttttcttt
     2281 taattcggta aagtcaaccg gtttggtcag gaaatcatcc gcccctaatt gtttggcggt
     2341 gttgaagttt tccgcgtccc cgtaagcagt aatcatcatt accacgggcg gaggtttaaa
     2401 gtacttggat ttgatctgcc ccagcagttc aagtccactc attcccggca tattgatgtc
     2461 ggagagtatc agcacggctt cctgttcatg ctcattcagg tatttcagtg cctgttcgcc
     2521 ggaaaaagca aaatcaaaat gcatttcacc gtttttaatt tcacggcgga atcgctgttc
     2581 aaataaggtc tgaacatctg tttcgtcgtc tacaactaag attttcatca ttggttcaat
     2641 gtttaaatgg tattgtaatt ctgaattcag catattctcc ttcttgggta tccacctgca
     2701 attgcccgcc atgcccttta gtaatgattt cataactcat actgagcccc agccctgttc
     2761 cttcaccggt gggtttggtg gtaaaaaatg gctggaaaat tttatccagt actttttgcg
     2821 gcaccccgtt tccattgtcc cgtaccctta tttcaatggc atttcccagg tttttagtag
     2881 ccacttcgat catcggttca tacccttcgc caacctgttt acgtttctgg gctacggaat
     2941 aaaacgagtt ggtgatcagg ttcagtaata cccgtccaat atcctgtggc gctacctgta
     3001 tatcgggcaa tccttcttca aaatgggttt ctgtttttga attgaacgaa tgatccttgg
     3061 ccctgaagcc atgataactc aggcgcaggc attcatcggc caggttattg atatcggtta
     3121 gttctttctg cccgctgctg ttccggctgt gctgcaacat accttttaca atcgagtcgg
     3181 ctcttttgcc atgaaaggat atcttctcat tattttgttt gatatcctca gccagctgca
     3241 ccgcttccgt cgtattccct ttgctgaatt cctccttcat ttcatcgatc agctcggtat
     3301 ttacttcgga aaaattgttg acgaagttca acggattctg gatttcatga gcgataccgg
     3361 cagtgagttc acccagcgaa gccattttct ccgattggat taactgggcc tgggtggatt
     3421 tcagtatttc aatggtctta ttcaattcct ttgtccgttg aaatacctgc tgttccagtt
     3481 cgatattacg cttactggtc tccgcattgg tcatataatt ctgcccggcc agccgataat
     3541 caggcatgga tccatgcaca tgaactatca gccaatggtc acctgttttc ttcagtac
//
LOCUS       DAOPMW010000360         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_30978_length_3593_cov_5.585359, whole genome shotgun sequence.
ACCESSION   DAOPMW010000360 DAOPMW010000000
VERSION     DAOPMW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30978_length_3593_cov_5.585359"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLU11_13510"
     CDS             <1..536
                     /locus_tag="PLU11_13510"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPN60197.1"
                     /translation="TQGIKNIMGSDADFSKWKFYVADNLNLKLKGQLRYRAGVGGFLN
                     NRSVELPDYQHFNGTITRFVPEYLRSFQFLPQYLFSNKDKFYSEAHIEYNLKGFLTNK
                     LPLIRNLNLYLVTGANGIWLNNHKYYYEVFAGFDNIFKQIRVDFVQSYQNGKPFQHGI
                     RIGLTKLNLSGGREDWP"
     gene            696..1733
                     /locus_tag="PLU11_13515"
     CDS             696..1733
                     /locus_tag="PLU11_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HPN60198.1"
                     /translation="MALHNRVSRKELKEALLNDPTPRTTISFYCYFKIEDPAVFRNML
                     YRELKQLGVLGRIYVANEGINAQISAPSANLEALRAYLYTIEPLNGLRLNIAVDDDGK
                     SFFVLDIKVRKKIVADGIEDPLFDMANKGKYVNAEQFNQLTEDPDTVVIDMRNYYEYE
                     VGHFKNAIEIPSDTFREQLPMAADMMQEKKETNIIMYCTGGIRCEKASAYMLHQGFKN
                     VFHLEGGIINYAKQIKEKGLPSKFIGKNFVFDERLGERITEDIIAHCHQCGKPADTHT
                     NCKNDGCHLLFIQCEECAKAHEGCCSKECQDTLLLPDERRKELRKGVDKGQHIFNKSR
                     QRLRPRLGDES"
     gene            complement(1730..2821)
                     /locus_tag="PLU11_13520"
     CDS             complement(1730..2821)
                     /locus_tag="PLU11_13520"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HPN60199.1"
                     /translation="MILITGFTASAQNYYLLVGTYDSPKSEGIYVYRFNTSDGSSSPV
                     SHIITSNPSYLSIAPGGQYVYAVHENAKEGKGGEVAAFSFNNKTGELQFINQQGTGGD
                     HPCYVETDQTGKWVFAGNYTSGSLSVMPVLPGGAVGAFSSHIAHEGNGPSDRQKSPHV
                     HCTLISPDNKTLLVPDLGIDKVMIYQFDVVTGKLTPGKQSFAASEPGAGPRHFTFHPN
                     QRFGYLIEELSGTVVAYTFKKGKLKQFQRISTMPAGDTQFPGSADIHVSQDGKFLYAS
                     NRSNSNTIAIYAIDKKGRLTLAGHQSTLGNGPRNFNFDPTGKFLLVGNQNSDEIVIFE
                     RNPDTGLLKDTGKRIALGKPVCIKWIPAG"
     gene            2981..3397
                     /locus_tag="PLU11_13525"
     CDS             2981..3397
                     /locus_tag="PLU11_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60200.1"
                     /translation="MKRIFTLTISFLLASVFLTSCIRETIYENNDNYWLSKEMGEVVY
                     SDPYCNYYVVETFNGYNIVRTYGGYKPYEGSIVYGNFSAAGTRDFYNRSSGVIYTGTV
                     TDYWMSYVDAQYALDYYCPVGKGATREFKKAVSLKK"
BASE COUNT          974 a          800 c          848 g          971 t
ORIGIN      
        1 acacgcaggg gatcaaaaat ataatgggca gtgatgccga tttcagtaaa tggaagtttt
       61 atgtggcgga taacctgaac ctgaagctga aagggcaact ccgctaccgg gccggggtgg
      121 gtggctttct gaataaccgt tcggtggaat tgcccgacta ccagcatttc aacggaacca
      181 tcacccggtt tgtcccggag tacctgcgga gcttccagtt tttgccgcaa tacctgttca
      241 gcaataagga caagttttat tccgaagcac atatcgaata taacctcaaa gggttcctga
      301 ccaataagct gccgctgatc cgcaacctca atctttacct ggtcaccggg gcaaatggga
      361 tctggttgaa taatcacaaa tattactatg aagtctttgc cgggttcgat aatatcttca
      421 agcagatccg ggtggatttt gttcaatcct accagaatgg caagcccttt caacacggaa
      481 tccggatcgg gttgactaaa ctgaatctca gtggtggccg ggaagactgg ccataattct
      541 ctgataatca ctgctgtctg ctgttccccg gaatttttgt aattttgcag cactgaaaag
      601 cccaacccgg atatttcagt gaagtttggt tcagcctgca actgtccaat tatttattaa
      661 ttatcccgca cctgcgggat tcagatttca acactatggc cttacacaac cgcgtctccc
      721 gcaaggagct gaaggaagca ttattgaacg atcccacacc gaggaccacg atttcctttt
      781 attgttattt taaaattgaa gatcccgctg tattccggaa tatgctttac cgtgagctga
      841 agcaactggg tgtactggga cgaatctatg tggccaatga ggggattaac gcgcagatca
      901 gcgccccttc cgccaacctg gaggcattac gggcttacct gtatacgatc gaaccattga
      961 atggactgcg gctgaatatt gccgtggatg atgatggtaa atcttttttt gtcctggata
     1021 tcaaagtgcg gaagaagatc gtagccgatg gcattgagga tcccttattt gatatggcca
     1081 ataaagggaa gtatgtgaac gcggaacaat tcaaccagct caccgaagat ccggataccg
     1141 tggtgatcga tatgcgtaat tactatgagt atgaagtagg acattttaaa aatgcgattg
     1201 agatcccttc agacacattc cgggaacaat tgcccatggc ggctgatatg atgcaggaga
     1261 aaaaggagac caatatcatt atgtattgta ccgggggtat ccgttgtgag aaagcctccg
     1321 cttatatgct gcaccaggga tttaaaaatg tctttcacct ggaaggcgga atcatcaatt
     1381 atgcaaaaca gataaaagag aaaggcctgc ccagcaaatt catcggaaag aactttgtct
     1441 ttgacgaaag actgggggag cggattacgg aggatataat cgcccattgt catcagtgcg
     1501 gcaaaccggc cgatacccat accaattgca agaatgatgg ctgtcatctc ctgttcatcc
     1561 aatgcgagga atgtgccaaa gcccatgaag gctgttgcag caaggaatgc caggatacgc
     1621 tgctgcttcc ggatgagcga cggaaggagt taagaaaagg cgtggataaa gggcagcata
     1681 tttttaataa atcccgtcaa cggttaaggc cccgtctggg agatgaatct taacctgccg
     1741 gaatccattt aatacaaacc ggcttgccca acgcaattcg tttgccggtg tcttttaata
     1801 agccggtatc cgggttacgt tcaaagatca cgatctcatc actgttctga tttcccacca
     1861 gcaggaattt tccggtagga tcaaaattga aattgcgcgg accgtttccc agggtggact
     1921 ggtgtccggc cagggtcagt ctgccttttt tatcaattgc atatatcgca atcgtatttg
     1981 aattgctccg gttagaggca tacaggaatt tcccatcctg gcttacatgg atatccgcac
     2041 tgcccggaaa ttgggtatcg ccggccggca tagtactgat ccgttgaaat tgtttcagtt
     2101 ttcctttttt gaatgtataa gcgactacgg ttccactcag ctcctcgatc aggtaaccga
     2161 atctttgatt gggatgaaat gtaaaatgac gcgggccggc accgggttca gaagcggcaa
     2221 aggattgttt cccgggagtc agtttaccgg taactacatc gaactgatag atcatgactt
     2281 tatcaatacc caggtcgggc accaggagcg tcttgttatc cggactgatc agcgtgcaat
     2341 gtacatgtgg cgatttctgc cgatcggagg ggccattgcc ttcatgcgca atatgcgaac
     2401 taaaggcacc tactgctccg cctggtaaga caggcatcac cgataaactg ccgctggtat
     2461 aattgccggc aaagacccat ttgcctgtct gatcggtttc tacataacag ggatgatctc
     2521 cacccgtgcc ttgctgattg ataaactgca gttcgccagt tttattattg aaagagaagg
     2581 ccgctacttc accgccctta ccttctttgg cattttcatg tacggcataa acatattgtc
     2641 cgcccggcgc aatggacagg taagaaggat tggatgttat aatatgactt acggggctgg
     2701 aactgccatc cgaagtatta aaacgataca catagattcc ttcactcttt ggcgaatcgt
     2761 aagtgcccac caacaggtaa tagttttgtg cactggcggt gaatcctgtt attaaaatca
     2821 taaatgccaa tatccggtgt aacataacgg ggattgttta gctggtgaat ttcagtcaaa
     2881 gttttcactt taacaaacgc taaacaatcc tttttgaatc tggcacagtc tttctattat
     2941 aacaggtgtt agtcaattat tcaaccttaa atcaaaagcc atgaaacgga tttttacact
     3001 cacgatctct tttttacttg cctctgtttt tctgaccagt tgtatcaggg aaaccattta
     3061 tgaaaacaat gacaactatt ggttgtctaa ggaaatgggc gaagtggtat acagtgaccc
     3121 ttactgtaat tactatgtag tggaaacctt taacgggtac aatatcgtac gcacctacgg
     3181 cggctacaaa ccttatgaag gcagtattgt atacggcaac ttcagtgccg cgggtacccg
     3241 tgatttctat aatcgttctt ccggggtgat ctatacagga accgttactg attactggat
     3301 gagctacgtg gatgcacagt atgcactgga ttattattgt ccggtaggta agggggccac
     3361 ccgtgaattt aaaaaagcgg tctcattgaa aaaataaatg aagtggcata aaaaagaggc
     3421 ggtcagatgg ccgcctcttt ttatttaaaa acctgcacat cagttcaggt tattcaacgc
     3481 agtgttgata cggctgaatg tttcctcatc cggtaaagct tccggccgga atggtttccc
     3541 gatcactttt tcgtacagct caatgtagcg tttggaaatc gtgttcaccc att
//
LOCUS       DAOPMW010000361         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31059_length_3586_cov_14.168508, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000361 DAOPMW010000000
VERSION     DAOPMW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31059_length_3586_cov_14.168508"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLU11_13530"
     CDS             <1..642
                     /locus_tag="PLU11_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPN60201.1"
                     /translation="FDDGPNKGTRNVLEVIKTEQVAASFFVVGQHTLDTRQQKETWEM
                     LKADAGIELCNHSYSHARNRYTSFYESPEGVINDFNQSKKLLGFTNNVARMPGRNAWR
                     IDSIDHTDIMESKAAIDSLHASGYAIMGWDIEWTFNHKTLAPDADTALLLRRIYNMLE
                     ANKTKTPGHLVLLAHDQAYQQAEYVENLRYVIKQLKNNPEYELLLASKYPGVR"
     gene            complement(751..1554)
                     /locus_tag="PLU11_13535"
     CDS             complement(751..1554)
                     /locus_tag="PLU11_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPN60202.1"
                     /translation="MAETPRVNHDIDYKIYGEELQFVEIELDPQETAIAESGAMMMME
                     DGIQMQTIFGDGSTQQSGGFLGKLMSAGKRILTGESLFMTAFTNAVQGKKKVSFAAPY
                     TGKIITLDLQELGGTIIAQKDAFLCAAKGVSVGIHFQRKLGTGLFGGEGFIMQKLEGD
                     GFAFLHAGGFIVERILKPGEVLKIDTGCVVAYTPDVDFDIEFVRGIKNFMFGGEGLFF
                     ARMQGPGKVWIQSLPISRLAGRIMQYGTYNRKEEGSLLGGIGNIFDGRE"
     gene            1722..>3586
                     /locus_tag="PLU11_13540"
     CDS             1722..>3586
                     /locus_tag="PLU11_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPN60203.1"
                     /translation="MKRVLLLTPFFFLLALLANGQTKLVEKITRQGNQIVIPYEKYVL
                     PNGLTLVVHEDHSDPVVHVDVTYHVGSAREEIGKSGFAHFFEHMMFQGSDHVADEQHF
                     KIVSDAGGTLNGSTNRDRTNYYETVPSNQLEKMLWLEADRMGFLLDAVTQKKFEVQRS
                     TVKNERGQNYDNRPYGLAGETSSRSLYPYGHPYSWLTIGYVEDLNRVDVNDLKNFFLR
                     WYGPNNATVTIGGDVKPAEVVKLVEKYFGSIPRGPEVKPVQVPRAILNSDRYVSYTDN
                     YARLPLLSISFPTVPNYHKDMGALACLAQVLGQGKNAVLYQQLTKKQLAVQASAFSSL
                     SELAGELSFQVVPSPGKTLAKMDTLFRAALDSFEARGVTDEDVSKFKGGIEAQQINGL
                     QSVSGKVSQLAAFQTFAGNPNKIADLIQMYQSVTKEDVMRVYNEYIKGKHAVFLSVLP
                     KGQENLIAAANNYTIDSSKYDAPDYGYNGLKYAKATDNFDRSKIPGTGANPVVKVPAF
                     WKKDLPNGIKMIGTESNEIPTVTLTITIPGGHLLQANDTAKVGLAGMFGSMMNEDTKN
                     FTAEQMAVELQKLGSNVSVSSSFDGITFSVQSLKKNLDKTLTLVQEKMFNPKFTEA"
BASE COUNT         1024 a          863 c          802 g          897 t
ORIGIN      
        1 tttgatgacg gccccaacaa aggcacccgc aatgtgctgg aagtgatcaa aaccgagcag
       61 gtagccgcca gtttttttgt agtaggtcag cacacattgg atacccgcca gcaaaaagaa
      121 acctgggaaa tgctgaaagc ggatgcgggt attgaactgt gtaatcacag ttatagtcat
      181 gcccgtaacc ggtatacttc attttacgaa agtccggaag gcgtaatcaa tgattttaac
      241 cagtcaaaaa aattattagg attcaccaat aacgtagccc gtatgcccgg ccgtaatgcc
      301 tggcggattg atagtattga ccacacagat attatggaaa gcaaggcggc catcgactcc
      361 ctgcatgctt cgggttatgc catcatgggc tgggatatcg aatggacctt taaccataaa
      421 accctggcac cggatgcgga taccgcgttg ttattacgcc ggatctataa tatgctggag
      481 gcgaataaaa caaaaacacc cggtcacctc gtgctgctgg cacatgatca ggcttatcag
      541 caggcggaat atgtggaaaa tctgcggtat gtaattaagc agctaaaaaa taacccggag
      601 tatgagttgc tgctggccag taaatatccg ggggtcagat aagtcaaaat taaaaagtca
      661 aaattaaaaa aacggagcgt ctgatttttg taatgacttt ccggtttaat gattttgact
      721 ttttaatttt tacttttgac tttaatgagg tcactctctg ccatcaaaaa tattcccgat
      781 cccaccgagc aaactacctt cctcttttcg gttatacgta ccatattgca tgatccggcc
      841 ggcgaggcgg ctgatgggca gagattgtat ccatacttta ccgggcccct gcatgcgggc
      901 aaagaataaa ccttccccgc caaacataaa gttcttgatg ccccttacaa actcgatatc
      961 gaaatccaca tccggggtat aggctaccac gcatcccgta tcaattttca atacttcacc
     1021 gggtttcaga atccgctcca cgatgaatcc acccgcatgg agaaaagcaa atccatctcc
     1081 ttcgagtttt tgcataataa agccttcgcc accaaataaa cccgtcccca gcttacgttg
     1141 aaaatgaata cccacactca cgcctttagc cgcacataaa aaagcatctt tctgcgcaat
     1201 gatggttccg cccagttcct gcagatccag tgttataatc ttgccggtat aaggagcagc
     1261 aaaactcact ttctttttac cctgtaccgc attggtaaaa gccgtcataa aaagactctc
     1321 cccggtgagt atccgtttac cggcactcat cagttttccc agaaaaccac cggattgctg
     1381 cgtcgaccca tctccaaaaa tggtttgcat ctgaatgcca tcctccatca tcatcatcgc
     1441 cccgctttcc gcgatggcgg tctcctgcgg gtccagttcg atctctacaa attgcaactc
     1501 ttcaccatat attttgtaat cgatatcatg atttaccctt ggtgtttcag ccatagttgt
     1561 attttttttt tacaaagtta aacaggtcgg ggatatccgg accggctttt ccgcgaacgg
     1621 aggaaaattg ccggtgaaaa tgataaatgg catcgggata gggtatttat ggtgtaattt
     1681 gcctgaccta aatgcttcac tgcactttca aaaaaattaa tatgaaaaga gtcctgctac
     1741 tgacgccctt tttctttttg ctggccctgc tggcaaatgg tcaaaccaaa ctggtggaga
     1801 aaataaccag acagggaaac cagatcgtga ttccctatga gaaatatgtt ttacccaacg
     1861 gactgaccct ggttgtccat gaggatcaca gtgaccctgt ggtacatgtc gatgttactt
     1921 accatgttgg ttccgcccgg gaggaaatcg ggaaatccgg ttttgcgcac ttttttgagc
     1981 acatgatgtt ccagggcagt gatcacgtag ccgacgaaca acattttaaa attgtttctg
     2041 atgccggagg tacactcaat ggaagtacca accgcgaccg caccaattat tatgaaaccg
     2101 tacccagcaa ccagttggaa aaaatgctct ggctcgaagc cgaccggatg ggctttctgc
     2161 tggatgcggt cacccaaaag aaatttgaag tacagcgttc tacggtaaaa aatgaaagag
     2221 gacagaatta tgataaccgt ccatatggac tggccggcga aacctcttcc cgcagccttt
     2281 atccctatgg ccacccctac tcctggctga ctatcggtta tgtggaagat ctgaaccggg
     2341 tagatgtaaa tgatctgaaa aactttttcc tgcgctggta tggccccaat aatgccactg
     2401 tcactattgg tggggatgta aagcctgcag aagttgtgaa actggtagaa aaatatttcg
     2461 gttccattcc ccgcggaccg gaagttaaac cggtacaggt tccccgggcg atcctaaaca
     2521 gcgaccgcta tgtatcctat accgataact atgcccgatt gcctcttctt tcaattagtt
     2581 ttcctaccgt accaaattac cataaggaca tgggggccct ggcctgtctg gcccaggtac
     2641 tcggacaagg aaagaatgct gtcttatacc agcaactgac taaaaaacaa ctggccgttc
     2701 aggccagtgc attcagttcg ctctcagaac tggccggtga attaagtttc caggtggtgc
     2761 cttctccggg caagaccctt gcgaaaatgg ataccttatt ccgggcagct ttggattcct
     2821 ttgaagccag gggtgtaacg gatgaagatg tatcaaaatt taaagggggg attgaagcgc
     2881 agcaaatcaa cggactgcag agtgtatccg gcaaagtatc ccagctggcc gccttccaga
     2941 ctttcgcagg taaccccaat aaaatcgccg acctgatcca gatgtaccag tccgtgacca
     3001 aggaagatgt gatgcgcgtg tataatgaat atatcaaagg caaacatgct gtgttcctga
     3061 gtgtattgcc caaaggacag gagaacctga ttgctgccgc caataattat accattgact
     3121 ccagcaaata tgatgcgcct gactatggtt acaacggttt gaaatatgcg aaagcaacgg
     3181 acaattttga ccgcagtaaa atacccggca ccggcgccaa tcccgtagtg aaagtgcctg
     3241 ctttctggaa aaaagatttg ccaaacggta ttaaaatgat cggaacggaa agcaatgaaa
     3301 tcccaaccgt tactttaacg attacaatac ccggcggaca cctcctgcag gcaaatgata
     3361 ccgctaaagt gggactggcc ggcatgtttg gcagtatgat gaatgaagac acgaagaatt
     3421 ttaccgctga acaaatggcg gtggaactgc aaaaactggg cagcaatgtt tccgtcagta
     3481 gcagttttga cggcatcact ttcagtgttc agagcctgaa gaaaaatctg gacaaaacac
     3541 tcacactggt ccaggaaaaa atgtttaacc cgaaatttac cgaagc
//
LOCUS       DAOPMW010000362         3580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31139_length_3580_cov_9.831773, whole genome shotgun sequence.
ACCESSION   DAOPMW010000362 DAOPMW010000000
VERSION     DAOPMW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31139_length_3580_cov_9.831773"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLU11_13545"
     CDS             <1..315
                     /locus_tag="PLU11_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60204.1"
                     /translation="PNVVTSQNAVAVQSVPKNNIPKGILTKTDVTNNTGSSPDIKPGI
                     DFAVANSDEQTPEIEDNDGKKNKLRGFFRKVTRTFEKRTNIDPTDQDNRLLVGSLAIK
                     LK"
     gene            379..1221
                     /locus_tag="PLU11_13550"
     CDS             379..1221
                     /locus_tag="PLU11_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN60205.1"
                     /translation="MKRIFTFCMALLMTLCSVAQTDTTAVPKTAPHDDTIRIGGMVII
                     RKAGSKDREIIHDREYRMRNRRVEKPSNLSTNWWIVDIGFSNYTDNSNYVNSAFDGFT
                     ASGITKDDLVARAGKSRNVNLWFFMQKLNIAKHVLNLKYGMGLELNNYHFENDKLLFS
                     KNPTLISEGSTSFKKVKLAVDYLTVPIMLNINFTPNRKNGFGISGGVSAGYMYSARFK
                     TKVDGDKNKVRSDFDLERFKLSYIGELTLGPVKLYGSYAMNNMWDKSLDMTPYTLGFR
                     FSNF"
     gene            1499..2059
                     /locus_tag="PLU11_13555"
     CDS             1499..2059
                     /locus_tag="PLU11_13555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPN60206.1"
                     /translation="MKQIVFLLSAFLGSSLTATDYHSSRPITNNKRFSANAGPVGRVS
                     IMIDKSDYELTVYDEEGWYATYPVVFGNNSLEDKKMEGDKNTPEGSFRITTKRVHDKW
                     CRFLAIDYPTKESIEKFKRRKQTGEIPANAKIGGGIGIHGVWPHEDFVIDRYKNWTLG
                     CISMKNEDVKDLYQYIGSGTQVTIRK"
     gene            complement(2067..3053)
                     /locus_tag="PLU11_13560"
     CDS             complement(2067..3053)
                     /locus_tag="PLU11_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN60207.1"
                     /translation="MKETKSSRRLPALFRWIGWVLLFQLVLINISAAIYAHRLTHFYN
                     DPSLQGSHSSGNIFRKTWKLFTGPKFPKSVIRQTPSFSYDTVRLMRSNGNWVDAWYGE
                     PDSLARGTVIMFHGLSASKSYMVREAATFRLMGYKVMLVDLRAHGNSDGQNTSIGRKE
                     TEEVQLAYNYIQSKGEKSIFLWGVSMGAVVVARSVAVGAVQPAGIIMELPFESLQSHL
                     KARARVLGFPQQPFAFLVTGWIGIESGFNGYSHSTVNYAKKINCPVLLQYGAKDTYVL
                     KSEIDGIYKAIPGNHKNLVGYEEAGHESLLMVDPERWKETTGSFLLQNQRQG"
     gene            complement(3164..>3580)
                     /locus_tag="PLU11_13565"
     CDS             complement(3164..>3580)
                     /locus_tag="PLU11_13565"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HPN60208.1"
                     /translation="NYRITGRVDDVLNVSGHRLGTAEVENAINMHAGVVESAVVGYPH
                     DIKGQGVYAYVIFEHVHGDENLTRQDILQTVTRVIGPIAKPDKIQFVPGLPKTRSGKI
                     MRRILRKIAEGEMENLGDTSTLLDPGVVDEIKKGKL"
BASE COUNT         1025 a          849 c          784 g          922 t
ORIGIN      
        1 ccgaatgttg tcacaagtca aaatgctgta gctgtacagt ctgttccaaa aaataatatt
       61 cctaaaggca ttttaacaaa aacagatgta acaaataata caggttcttc gcctgatata
      121 aaaccgggta ttgatttcgc agttgccaac tctgatgaac agactcctga gatagaagat
      181 aatgatggta agaaaaacaa actccgtggc ttcttccgga aggtgacacg gacttttgag
      241 aaacgaacca acattgaccc gaccgatcag gacaaccggt tactggttgg cagtttggca
      301 atcaagttaa aataatccgg gcccagatgg cgacggatga tcttcaaaaa acattattaa
      361 tcgaagaaaa caaataacat gaaaaggatt tttactttct gtatggcttt attgatgaca
      421 ctttgcagtg ttgcacaaac cgataccacg gctgtaccta agacagcgcc acatgatgat
      481 acgatccgga tcggcgggat ggttattatc cgtaaagccg gtagcaaaga ccgtgaaatt
      541 attcatgacc gtgaataccg gatgcgcaac cgccgtgtgg aaaaaccatc taacctcagt
      601 accaactggt ggatcgtgga tatcggtttc tccaactaca ccgacaattc caattatgta
      661 aattccgcat ttgacggctt taccgccagc ggtattacca aagacgacct ggtagccaga
      721 gccggtaaat cacgcaatgt gaatctctgg ttcttcatgc aaaaactgaa tattgccaaa
      781 catgtgttga acctgaaata cgggatggga ctggagttga acaattatca ttttgagaat
      841 gacaagctcc tgttttctaa aaaccccacc ctgatcagcg aaggcagtac ctcctttaaa
      901 aaagtaaaac tggcggttga ctacctgacc gtacccatca tgctcaacat caatttcacc
      961 ccgaaccgta aaaacggatt tggcatcagc ggaggtgtga gtgcgggtta tatgtattcc
     1021 gcacgtttta aaacaaaagt ggatggtgac aaaaataaag tcaggagtga ttttgacctg
     1081 gaacgattca aattgtccta tatcggggaa ttaacccttg gacccgtgaa actgtatggc
     1141 agttatgcca tgaacaatat gtgggataag agtcttgata tgaccccata tacgctgggt
     1201 ttccgcttca gcaactttta agaatttctc atcttcatac attaccggcc tgttcccagg
     1261 ccggtttttt tattttccta agcaggttta ccgggtaaaa ggccccgtaa attttctaaa
     1321 cgggctgtta aattgaaaca gacgggtact atagttatcc accaccggaa aataaaaaat
     1381 ctggcactcc gtttgaacgg taggacggac caccttttat ttttctaaag ccatggaatc
     1441 aatgctgctc ccaccaggtg gcgggttaca accggcttta ttgaaaacac taatggacat
     1501 gaaacaaatc gtatttcttc tttccgcatt tttgggcagc agccttacag caacagatta
     1561 tcatagctcc aggccgatta ccaacaataa gcggttctcg gcaaatgctg gtcctgttgg
     1621 acgggtgtcc attatgatcg ataaatcaga ctatgaactt accgtatatg acgaagaggg
     1681 ctggtatgcc acctacccgg tagtattcgg gaataacagt cttgaggata aaaaaatgga
     1741 aggggataaa aacacaccgg aaggcagttt ccggatcacc accaaaaggg tgcatgataa
     1801 atggtgccgg tttctggcaa tagattatcc cacgaaagaa agtatcgaaa aattcaagcg
     1861 gcggaaacaa accggagaaa taccggccaa tgcaaaaatt ggtggcggta ttggtataca
     1921 tggcgtctgg ccacacgaag attttgtgat tgaccgctat aagaactgga cccttggctg
     1981 tatctccatg aagaatgagg atgtaaagga cctgtaccag tatatcggat cgggaaccca
     2041 ggtcaccata cgcaaataag caacgattaa ccctgacgtt gattttgcaa caggaaagaa
     2101 cctgttgtct ctttccatcg ttccggatct accattagca gggattcgtg acctgcttct
     2161 tcatacccca ccaggttttt atggtttccg ggaatggctt tataaatccc atcaatttct
     2221 gacttcaata catatgtatc ctttgcgcca tattgcagca aaaccgggca attgattttc
     2281 ttcgcatagt tcaccgtgga atgactgtag ccattgaaac cactttcaat gccgatccat
     2341 ccggttacca gaaatgcaaa tggttgctga ggaaagccta atacacgggc cctggctttg
     2401 agatgtgact gcaatgattc aaatggcaat tccataatga ttcctgcagg ctgaaccgca
     2461 ccaaccgcca ctgaacgggc cactactact gctcccatcg atacacccca taagaaaatg
     2521 gatttttccc cttttgactg aatataatta taagccagtt gcacttcttc cgtttccttc
     2581 cgtccgatgc tggtgttctg cccgtcactg ttcccatgcg ccctcagatc aaccagcatc
     2641 accttgtaac ccatgagccg gaaagtagcg gcctcccgaa ccatatagct tttactggca
     2701 ctcagtccgt gaaacatgat cactgtgccc cgggccaggg aatctggttc gccataccag
     2761 gcatccaccc agtttccgtt ggaacgcatt aaacgtaccg tatcgtagga aaaggaaggt
     2821 gtttggcgaa taaccgattt tggaaatttt ggtccggtaa atagtttcca ggttttacgg
     2881 aaaatattcc ccgaagaatg actgccctgt aaagagggat cattataaaa atgggtcagc
     2941 cggtgagcat agatggcggc actgatattg atcagcacca actggaaaag caggacccat
     3001 ccgatccaac ggaacaaggc gggtaagcgg cggctggact ttgtttcttt cataaggtgc
     3061 tgtttccggc aagcgttatc cgccaaaaat aatttttacc cgccacagaa acgactattt
     3121 accttctttt ttataaccag cagaaaaaac aggggcaact ggctcagagt ttaccctttt
     3181 taatctcgtc cacgacaccc ggatccagta aggtggacgt atcgcccagg ttctccattt
     3241 ccccttccgc gatctttcgc aggatacgcc gcatgatctt accgcttctt gttttgggca
     3301 atccgggtac aaactggatt ttatccggtt tggcaatggg accgatcacg cgggttacag
     3361 tctgcaggat atcctggcgg gtcaggtttt catctccatg tacatgttca aagatgacgt
     3421 aggcgtaaac tccctgtccc ttgatatcat gcggataacc cacgactgca ctttccacca
     3481 ccccggcgtg catattgatc gcattttcca cttcggcagt gccaagccgg tgtccgctca
     3541 cattcaatac atcatccacc cggccggtaa tacggtaatt
//
LOCUS       DAOPMW010000363         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31187_length_3575_cov_6.740341, whole genome shotgun sequence.
ACCESSION   DAOPMW010000363 DAOPMW010000000
VERSION     DAOPMW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31187_length_3575_cov_6.740341"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLU11_13570"
     CDS             complement(<1..313)
                     /locus_tag="PLU11_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60209.1"
                     /translation="MNQSRKFLVISLFAAFSAGFMACDKPGNDVPENPSGPYKLSYGD
                     SIIYLKPGSGDYIVNPVTTRPGEYTGFPDGIEIDETTGAINVSKSETGLRYRITHTSP
                     TG"
     gene            484..1686
                     /locus_tag="PLU11_13575"
     CDS             484..1686
                     /locus_tag="PLU11_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN60210.1"
                     /translation="MKRIGLIREGKIPADNRVALTPAQCKWILKNSPDIQIVVQSSPD
                     RCFSDREYISAGAELKEDLSDCDILFGIKEVPVSQLIPGKTYLFFSHTKKKQPHNQPL
                     IKAVMDKKITLVDYECLEHEDGQRIIGFGFFAGIVGAHNGMMAYGLRTGLYKLDRVYK
                     QRSFRELIHNYFGLRLPNVKIAITGSGRVAHGILEIMNLMGIHEVENDEYLRRRFSYP
                     VYTQVKGAELYGHKVTGSYNRLDFHAHPDQYTCRFLPYAAQTDILMNGVYWDKNVPRL
                     FEKEEACSDEFIIQTIADITDDANGSVPINVGDQTIEDPIYGIDKKTLEKTAPYLPGS
                     IDIMAVGNLPNELPRDASRYFGEQLIKFILEDLLSNGSPIIERATIVKEGALTAEYEY
                     MREYAEGK"
     gene            complement(1689..2423)
                     /locus_tag="PLU11_13580"
     CDS             complement(1689..2423)
                     /locus_tag="PLU11_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPN60211.1"
                     /translation="MANSYFRFKQFTIHQDQSAMKVTTDGCLFGAWAAKAIHERKPEK
                     GRWLDIGTGTGLLSLMVAQQNPDNLIDAVEIEIAAAEEARKNILGAGKEKQIIVHQAD
                     IKQYHPAHQYDIIFSNPPFYEKELKGNNTEKNKAHHNEGLLFHQLLACCKQLLKKEGS
                     IFLLLPYKREKELLEKIKETGFVVHQHIRVRPAVRLDYFRMLIELQFNAEAPGAIVTG
                     ELAVKDEKDQYTPEFVGLLRDYYLYL"
     gene            complement(2427..3476)
                     /gene="tsaD"
                     /locus_tag="PLU11_13585"
     CDS             complement(2427..3476)
                     /gene="tsaD"
                     /locus_tag="PLU11_13585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPN60212.1"
                     /translation="MPVILAIESSCDETSAAICADGKVLSNFIATQAIHEQYGGVVPE
                     LASRAHMQHIVPVVDKALKEANHTLAEVDAIAFTQSPGLIGSLLVGGQFAKSLSLALD
                     KPLIAVHHMQAHVLANLIDDPKPAFPFLCLTVSGGHTQIVLCESPVKMKVIGETIDDA
                     AGEAFDKSAKLLGLPYPGGPLIDKYAKEGNPNRFPFPEPQIAGLNFSFSGLKTSILYF
                     LQNAGKSNLFKEEFTATEEEKQAFIQDNLADICASIQHRIVTILLNKVKKAAVETGIK
                     EICLAGGVSANSGLRNSFQEMGKKYNWNTYIPAFQYCTDNAGMIAITAYYKFLEGQTD
                     PITISSSARAAWSSD"
BASE COUNT          962 a          816 c          818 g          979 t
ORIGIN      
        1 cacctgtagg ggatgtatgc gtgatgcgat accggaggcc ggtttcactt tttgaaacat
       61 ttatagcacc ggtggtttca tcgatttcaa tgccatccgg aaaaccggta tactcgcccg
      121 gacgggtggt gaccgggttt acaatataat cacctgaacc gggttttaaa taaatgattg
      181 aatcgccata gctcagttta tacggccccg aaggattttc aggaacatca ttacccggct
      241 tgtcacaagc cataaagccc gcactgaaag ctgcgaataa gctgattaca agaaatttac
      301 gggactgatt catgggcctt ttttgtgggt aaccgccgtt tcaaaaaatg taaccggcag
      361 acagaaatga attacaaaat tttttcaaag ttacaacatt tattatgcca aaaagatgac
      421 cgaaatcagt ttgtctggca ttgatcaaat gggcaccttt gcggaactga aaatgaaggc
      481 catatgaaaa gaatcggact tatacgtgaa ggaaaaattc cggcagataa ccgggtggca
      541 ctgacccccg ctcaatgtaa atggatactc aagaatagtc ctgatattca aattgttgtg
      601 caaagttcac cagaccgttg tttcagcgac cgggagtaca tttccgccgg agctgaattg
      661 aaagaagacc tgtctgattg tgacatcctg tttggaatta aagaagtgcc tgtcagccag
      721 ctgattcccg gaaaaaccta cctctttttt tcgcacacaa agaaaaagca accccataat
      781 cagcccctga ttaaagctgt aatggataaa aaaatcaccc tggttgatta tgaatgcctt
      841 gaacatgagg atggacaacg gatcattggt tttggctttt ttgccggaat tgttggcgct
      901 cataatggca tgatggcgta cggactccgt acaggtttat acaagctgga ccgtgtgtac
      961 aaacaacgca gtttccggga actcatccac aattatttcg ggttgcgttt accgaatgtg
     1021 aaaattgcca taaccggttc aggccgggtg gcgcacggga ttctggagat catgaacctg
     1081 atgggtattc atgaagtgga aaatgatgaa tacctgcgcc ggcggttctc ttacccggta
     1141 tacacccagg taaaaggggc cgaactctac ggacacaaag taacgggcag ctataatcgc
     1201 cttgattttc atgcgcatcc ggaccaatat acctgcaggt ttttgccata tgctgctcag
     1261 acggatattc tgatgaacgg ggtgtactgg gataaaaacg tgccgcgttt gtttgagaag
     1321 gaagaagcct gctcggatga attcatcatt cagactattg cggatattac tgatgatgct
     1381 aatggttcgg ttcctattaa tgtcggggat cagaccattg aggatccgat ctatggtatc
     1441 gataagaaaa cactggaaaa aacagcgcct tatcttcctg gttccattga tatcatggcc
     1501 gtaggtaatc ttcccaatga actgcctcgt gacgccagcc gctatttcgg cgaacaactg
     1561 atcaaattca tattggaaga cctgctcagc aatggttctc cgatcattga acgggctacc
     1621 atcgttaaag aaggagcatt gaccgctgaa tatgagtata tgcgggaata cgctgaagga
     1681 aaataaattc agagatagag ataataatcc cggagcaacc ccacaaactc cggggtatac
     1741 tgatcctttt catctttaac cgccagttct ccggttacaa tagcgccggg tgcttcggca
     1801 ttgaattgta attcaatcaa catcctgaaa taatccagcc gcaccgccgg tctgacccgg
     1861 atgtgctgat gaactacaaa gcccgtttct tttatttttt ccagtagttc tttttccctt
     1921 ttatagggta ataagagaaa gatacttccc tctttcttca gcagttgttt acaacaggct
     1981 aacagttgat gaaacaggag tccttcgttg tgatgggctt tatttttttc cgtgttattt
     2041 cctttcagtt ccttttcata aaaaggcgga ttactgaaaa tgatatcata ttgatgagcg
     2101 ggatgatatt gcttgatgtc tgcctggtga acgataatct gtttctcttt tcccgctcca
     2161 agaatattct tacgcgcttc ttccgctgcc gcaatttcaa tttccactgc atcaatcaga
     2221 ttatccgggt tctgttgggc caccatcaga ctgagcaagc cagtacctgt gccgatgtcc
     2281 agccaacggc ccttttccgg ttttctttca tgaatggctt tggctgccca ggcaccaaac
     2341 aggcacccat cggtggtcac cttcatagcg gattggtcct ggtgaatggt aaattgtttg
     2401 aaacggaaat aggagttagc cataccttaa tcagatgacc aggcagccct ggcagaagag
     2461 gaaatggtga tcggatccgt ttgaccttcc aggaatttgt aataagcggt aatggcgatc
     2521 ataccggcat tgtcggtaca atactggaac gcggggatat aagtgttcca gttgtatttt
     2581 tttcccattt cctggaaact gttccgcagt ccactgttgg cagaaactcc tccggccagg
     2641 cagatctctt ttataccggt ttcaaccgcc gcttttttca ctttattcag gagtatggtc
     2701 acaatacggt gctggataga cgcacagata tcggccagat tgtcctggat aaaagcctgt
     2761 ttctcttctt cagttgcggt aaattcttcc ttgaataaat tactcttacc ggcattttgt
     2821 aaaaaataaa ggatcgaggt tttcaaacca ctgaaactga agttcagtcc agcgatctgt
     2881 ggttcaggaa aagggaaacg gttaggattt ccttccttcg catatttatc aatcaggggg
     2941 ccgcccgggt aaggaagtcc aagcagtttg gctgatttat caaacgcttc gcccgcggca
     3001 tcatcaattg tttcaccgat cactttcatt ttcaccggac tctcacataa tacgatctgt
     3061 gtatgtccgc cactcaccgt cagacaaaga aaggggaacg ccggtttggg atcatcaatc
     3121 aggttggcca gcacatgggc ctgcatatga tgtacagcga tcaggggttt atccagtgca
     3181 agtgaaagtg atttggcaaa ttgaccacct acgagcagcg atccgatcag tccgggtgat
     3241 tgtgtaaagg caatggcgtc cacctcggcc agggtatgat tcgcttcctt caacgcctta
     3301 tccactaccg gtacaatatg ctgcatatgg gctcttgaag ccagttccgg cactactccg
     3361 ccatattgtt catggatcgc ctgtgtggca ataaaattcg acaaaacctt tccatcagca
     3421 cagatagcgg ctgaggtttc atcacaactt gattcaatgg ctaaaatcac cggcatccag
     3481 caaaattatg taattagctt atttactgcg aatagcttct accggatcca tactcgccgc
     3541 ctgccgggca ggaataaatc cggcgattac gccca
//
LOCUS       DAOPMW010000364         3551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31479_length_3551_cov_6.827803, whole genome shotgun sequence.
ACCESSION   DAOPMW010000364 DAOPMW010000000
VERSION     DAOPMW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31479_length_3551_cov_6.827803"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLU11_13590"
     CDS             complement(<1..489)
                     /locus_tag="PLU11_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPN60213.1"
                     /translation="MEKLYIIKIGGNIIDDEAKLAAFLRDFAAIEEKKILVHGGGKLA
                     TRLAEKMGIEQQLVDGRRITDAETLKIVTMVYAGFINKNIVATLQANHCNAIGLCGAD
                     GDAILAHKRNHPVLDYGFVGDVDSINTDLISTLLNKNISVVFAPITHDQQGQLLNTNA
                     DTI"
     gene            complement(535..1497)
                     /locus_tag="PLU11_13595"
     CDS             complement(535..1497)
                     /locus_tag="PLU11_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HPN60214.1"
                     /translation="MKNFVSVHDVNNIDALVQRALAHKANPLAHKTLGANKRIGCLFL
                     NPSMRTRLSTQIAAQNLGMEAIVFNVGSEGWALEFEEEAIMSGTSVEHVKDAAPVFGK
                     YFDILAIRTFPSLKNREDDYSELYIKQFIKYAGIPVLSLESATLHPLQSLTDIITITE
                     TLQEKPLNRKPKIVLSWAPHVKPLPQCVANSFSQWVNAWGQADFVITHPEDYELSEEF
                     TKGATITHNQEEALKDADFVYVKNWSTYTDYGKIYESDPKWMLTNKKLEATNNAKVMH
                     CLPVRRNVELSDEILDGPNSIVTREAGNRVWAAQAVLESLLQNN"
     gene            complement(1499..2650)
                     /locus_tag="PLU11_13600"
     CDS             complement(1499..2650)
                     /locus_tag="PLU11_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN60215.1"
                     /translation="MNLFDVYPINEVTIVKAKGSYVWDDKGEQYLDLYGGHAVISIGH
                     THPHWVKRIEEQLEKIAFYSNSIKIPLQVQLAEKLGKVSGKDDYQLFLCNSGAEANEN
                     ALKLASFHTGRKKIVAFSKSFHGRTSLAVAVTDNKNYTAPVNETDNVIFLPFNDGEAL
                     EACFEKEGKNIAAVIIEGIQGVGGINVADDGFLRSIRSLCDKHGAVYIADSVQCGYGR
                     SGKFFSHDFAGVSADIYTMAKGMGNGFPVAGIIIAPHIKPKHFQLGTTFGGNHLACAA
                     ALAVLEIIEEEKLMGNAVMIGKYLRDELNAIPELKNVRGRGLMIGFDVPEELKDLKKN
                     LLWKQKIFTGEAKPNVIRLLPSLGLKKKDAEQFIESIKEEINLLTTPAS"
     gene            complement(2766..3131)
                     /locus_tag="PLU11_13605"
     CDS             complement(2766..3131)
                     /locus_tag="PLU11_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN60216.1"
                     /translation="MQNFKDLVVWERAHHFTLKVYEASRSFPKEEVYGLTSQLKRAAS
                     SIPSNIAEGCGKNTQSDFAHYLNIALGSANESEYFLILAKDLKYLELPAFEILSSTIN
                     EIKAMLISLITKVRGKTNN"
     gene            complement(3167..>3551)
                     /locus_tag="PLU11_13610"
     CDS             complement(3167..>3551)
                     /locus_tag="PLU11_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPN60217.1"
                     /translation="IGESLLQLQPVNDVDVHFVPWRGDFTRGIFISSTLHCDESLEDM
                     YKLFEEYYNGHPFTTVSREPIFLKQVVNTNKAVLQLEKAGTKLVVHSAIDNLLKGASG
                     QAVQNMNLVCGLPETTGLKLKANYF"
BASE COUNT          853 a          833 c          814 g         1051 t
ORIGIN      
        1 tatggtatcg gcattggtat tcagtaattg tccctgctga tcatgcgtaa tgggcgcgaa
       61 taccacggaa atatttttat tcagcagggt gctgatcagg tcggtattga tgctatccac
      121 atccccgaca aatccatagt ccaataccgg gtgattgcgt ttgtgcgcca ggattgcatc
      181 tccatcagcg ccacaaagac cgatagcatt gcagtgattg gcctgaagcg tagccacgat
      241 attcttattg atgaaacctg catacaccat ggtcacgatc ttcagggttt cggcatccgt
      301 gatccggcgg ccatctacca gctgttgttc aattcccatc ttttccgcca gccgggtggc
      361 cagcttgcca cctccatgta ccaatatctt tttttcctca atggcggcaa agtcgcgcag
      421 gaaggccgcg agcttggctt catcatcaat gatgttgcct ccgatcttta tgatatacaa
      481 tttttccatc tggttttatt tttcgcaggc tgcaaaataa cagacccgtg acggtcagtt
      541 attttgcagc aggctctcca ataccgcctg tgccgcccat acccggttgc cggcttcacg
      601 ggtcacaatg ctgttgggtc cgtcgaggat ctcatcactc agttccacat tacggcgcac
      661 cggcaggcaa tgcatgactt tggcgttgtt ggtcgcctcg agttttttat tggtcagcat
      721 ccatttcggg tcgctttcat agatctttcc gtaatcagta tatgtactcc agttctttac
      781 atagacaaag tcggcatctt tcaatgcttc ttcctggtta tgggtgatgg tggctccttt
      841 agtgaattct tcactcagtt cataatcttc cgggtgcgtg atcacaaagt ccgcctgtcc
      901 ccaggcattc acccattggg aaaaactgtt ggccacacat tgaggaaggg gcttcacatg
      961 cggagcccag ctcaatacga tcttcggttt gcggttcagc ggtttttcct gcagtgtttc
     1021 agtgatcgtg atgatatcgg tcagtgactg caagggatgc agggtagcgc tttccaggct
     1081 cagcaccggg atgcccgcgt atttgataaa ttgtttgata tataattcgc tgtaatcatc
     1141 ttcccggttc ttcaatgatg ggaaggtacg gatagccagg atatcgaaat actttccgaa
     1201 gaccggagcg gcgtctttca cgtgttccac agatgtacca ctcatgatgg cttcttcctc
     1261 gaactcaagg gcccagcctt cactgcctac attgaagaca atggcttcca tacccaggtt
     1321 ttgtgcagcg atctgtgtac tcagacgggt acgcatactg ggattgagga acaaacaccc
     1381 gatacgctta ttggcgccaa gtgttttgtg tgcaagcggg ttggctttgt gggccagggc
     1441 acgttgcacc agggcgtcaa tattgtttac atcatgaacg gaaacgaaat ttttcatatc
     1501 agctggcagg ggttgtaagg aggttgatct cctcttttat ggattcgata aattgttcag
     1561 catctttttt ctttaaacct aaagaaggta aaaggcggat cacattgggt ttggcttcac
     1621 cagtgaagat tttttgcttc cagagcaggt tcttcttcag gtccttcagt tcttccggca
     1681 catcaaaacc gatcatcagt ccgcgtcccc tgacattttt caattcagga atggcattca
     1741 gttcgtcccg caggtatttg ccaatcatga cggcattgcc catcagtttt tcttcttcga
     1801 tgatttccag aaccgccaat gccgcagcac aagccaggtg attaccacca aacgtagtgc
     1861 ccagctggaa atgtttgggt ttgatatgag gggcaatgat gatacccgct accgggaacc
     1921 catttcccat tcctttggcc atggtataaa tatcggcact cactccggca aaatcatgac
     1981 tgaagaattt accactccgt ccataaccgc attgtacact atcggcaata tacacggcgc
     2041 cgtgtttatc acaaagagaa cggatgctgc gcagaaaacc atcatccgct acattgattc
     2101 ctccaacgcc ttgaatgcct tcaataataa cggccgcaat gttcttgccc tctttttcaa
     2161 aacaggcttc gagcgcctct ccatcattga acggcaggaa aattacattg tcggtttcat
     2221 tgaccggggc tgtgtagttt ttattatccg tcaccgccac ggcgagggaa gtgcgtccgt
     2281 ggaaggactt gctgaatgcg acgatctttt tacgacctgt atggaaagaa gccagtttca
     2341 gcgcattttc attggcttcg gcaccgctgt tgcagaggaa taactggtag tcgtccttcc
     2401 cggatacttt gcccagtttt tccgccagct gcacctgcag gggtatttta atggagttgg
     2461 aatagaaagc gatcttttcc aactgctctt caatccgctt tacccaatgc ggatgggtat
     2521 gtccgatgct gataacggca tgtcctccat aaagatccag gtactgttcg cctttatcgt
     2581 cccatacata ggagcctttg gcttttacga tagtgacttc gttgataggg tatacatcga
     2641 ataaattcat gagagttgta agttttatga aaagagttgt aagttttaag taataagttg
     2701 taagtaacgg agtatgttat aagttttaag taataagttg taagtgacag attctggttt
     2761 ataaattaat tattagtctt tcctcggact ttggtaatga gtgatataag catggcttta
     2821 atttcattga ttgtcgatga caatatttca aaagctggta attcaaggta tttaaggtct
     2881 ttggcaagaa tcaggaaata ttcagattca ttggcggaac ccagtgctat gtttaagtag
     2941 tgggcgaaat cgctctgtgt gttttttcca caaccttcag caatattgga tggaatagaa
     3001 gatgcagctc tctttaattg gcttgttagt ccataaactt cttctttggg aaaactccgc
     3061 gacgcctcat acactttcaa tgtaaagtga tgggctcttt cccaaactac aaggtctttg
     3121 aagttttgca tacttattac ttacaactta taacttacca ctttccctaa aaatagtttg
     3181 ctttcaattt caatcccgtc gtctccggca acccacaaac aaggttcata ttctgcaccg
     3241 cttgtccgct ggcgcctttt aataaattgt cgatggcgga atgcactacc agtttagtgc
     3301 cggctttttc caattggagc acggctttat tggtgttcac cacctgcttg agaaaaatgg
     3361 gttcgcggct gacggtggta aaggggtgac cattgtagta ctcctcaaag agtttataca
     3421 tgtcttccag gctttcgtcg caatgcaggg tggaactgat aaagatgccg cgggtgaaat
     3481 cgccccgcca ggggacaaaa tgaacgtcca catcatttac cggttgtaac tgcaggagcg
     3541 attcaccgat c
//
LOCUS       DAOPMW010000365         3547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31521_length_3547_cov_5.368557, whole genome shotgun sequence.
ACCESSION   DAOPMW010000365 DAOPMW010000000
VERSION     DAOPMW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31521_length_3547_cov_5.368557"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..770
                     /locus_tag="PLU11_13615"
     CDS             252..770
                     /locus_tag="PLU11_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60218.1"
                     /translation="MKLVFKFLYYFCITFIVLIFLTAAIIRFSPLDFASKEYDEIFDI
                     LFFGGIPVAILLTACRIGFQSMDRTKKRNTIIIHFLLSLGCFFLFFLYALVSFGEGMC
                     LYSDEETLFVNRSNPSTTITLRSYGCGATDSGPSSLSVELRKMYSPVFCSYTKVDTTR
                     IDRKEWTRTTGH"
     gene            977..2410
                     /locus_tag="PLU11_13620"
     CDS             977..2410
                     /locus_tag="PLU11_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN60219.1"
                     /translation="MSFKINLRNLLDVDIHFSKGYYSTDNILVSKGDRVHTMLIAIVG
                     FFGFTYTIIGLIQAYHYQAILSFSCGIFALIAWLIHRKGYTLASKLVNFIQLIAFIAL
                     MFYFPSSPYGEHINDSVLAFYVPVLIGTLIAFQEKQRIYGRVLAVIILLLISLLVLTD
                     IHYPEHEPVAVLNGANFDMLINIIGAAAATFAEVAYILALNDRLNNSLIKTNHELDNF
                     IYIVSHDLRSPLLSTKGLIDLAKMKIDDREQVLKYLGLAGKSINNLDQIIHEILTYSR
                     NARTEIQEEEFELKDVVKEIFDGLRFSAGPGFSFQEEYSGETKIYADKNRLSTVLRNI
                     ISNSVKYKKKEIENPFVFLRFENNNHHCSFSITDNGEGIPLECQPRVFEMFYRGTSSA
                     TGTGLGLYICKEMLDKMDVLFAISSKPGEGTTFNIQLKKIATSLVKEKKAPALNPAKQ
                     SGSPTASLKLNEQLNSIQGLSSFNQAG"
     gene            2518..>3547
                     /locus_tag="PLU11_13625"
     CDS             2518..>3547
                     /locus_tag="PLU11_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPN60220.1"
                     /translation="MSRRLIPIFLILTCLCAAVNAQPARPDFNPIDLRARSIRYDDDI
                     VKLVQNLTSPYSDKLSKVRSIFIWITDHIRYDWKFFNKDEEIEIPECKSGQDCDEVLA
                     NWEKKYLANILRKGKAISDGYARLFTRMCDIAGLEAATINGHTKTKLYQIGNAGPVNH
                     SWNVIWLDTTWHLLDATWSAGYTTEDENTGKLNGFVKQYNDYYFLTPFAEFSRNHFPQ
                     YTRWVLKPGYTKDKFAANPYFDPDILSKIRLHSPQTGMINGKKGDTIHFKFEYTGTIE
                     KLQVNTNLYRNAEVWGWEQINKRKKIWVQDTFALKRQRYTPFIKTGNLYEFDYVIPEN
                     SLYYLDILF"
BASE COUNT          978 a          859 c          717 g          993 t
ORIGIN      
        1 tatttgatat gttttaccgg gctactactg ccgcgccggg taccgggctg ggtttataca
       61 tctgcaaaga aatgctggaa aaaatgaggg cttctttcca cctggattcc actgagggcc
      121 atggcaccag ttttaccttc acacttgagc aggcgccccg ggtggtttca gcataattca
      181 aaatcattcc atgttttttt attcggtaac ttaacatagg ttaacgaatc acccgtcata
      241 ttattcctga aatgaaactg gtatttaaat ttttatacta tttctgtatc acatttattg
      301 tgctgatctt cctgaccgca gctataatcc ggttcagtcc gctggatttt gccagcaaag
      361 aatatgacga gatattcgat atccttttct ttgggggtat cccggtcgct atcctgctga
      421 cggcctgccg gatcggattc cagtccatgg accggacaaa gaaaagaaac acgattatta
      481 ttcattttct gcttagtctt ggttgtttct ttctcttctt tttatatgca ctggtttcat
      541 ttggtgaagg aatgtgttta tactccgatg aagagacctt gtttgtaaat agatccaacc
      601 cttccactac aattacactc cgttcttatg gttgcggggc aactgacagc ggtccctctt
      661 ccctgtctgt tgaattaagg aaaatgtatt cgcccgtttt ttgttcctac accaaagtgg
      721 ataccacccg gattgaccgg aaggaatgga cccggactac gggccattaa ttgcccattc
      781 tccggaccgg taataaggga tccgggacaa ccatagcttt ttctaagtac caactactac
      841 ctgttactaa tcattcatca ttagttcctg ataatcaagt gtttttacaa cctgttttgc
      901 accccggtta ctggtatttc ccaggattcc ggaatatctt tataccggtt aagtagcacc
      961 aataattagt accctgatgt cttttaaaat taatctgagg aatttactcg atgttgatat
     1021 ccacttctct aaaggatatt acagcaccga taatatattg gtcagtaaag gggaccgggt
     1081 acacaccatg cttatcgcca tcgttggttt ttttggtttc acctatacta tcatcggcct
     1141 gatccaggca tatcattacc aggctatcct cagtttttcc tgtggaattt tcgccttaat
     1201 tgcctggctg atccaccgga aaggatatac gctcgcgtca aaactggtca acttcatcca
     1261 gctgattgcc ttcatcgcac tgatgttcta ctttccttcc tctccttatg gcgaacatat
     1321 caatgacagt gttctggctt tttatgtacc ggtgctgatt ggcacgctga tcgctttcca
     1381 ggaaaaacag agaatatacg gaagggtcct ggcggtaatc atattgctgc ttatctcctt
     1441 gctggtcctc acggatattc attatccgga acatgaaccg gtggcagtac tgaatggggc
     1501 caacttcgat atgctcatca atataatcgg agcagcagcg gccacgtttg ctgaagtggc
     1561 ttatatcctg gcgctgaatg accggctgaa taacagtctc atcaagacca atcatgaact
     1621 cgataatttc atttatatcg tttcacatga ccttcgctcc cccttgcttt ccaccaaggg
     1681 actgattgac cttgcaaaaa tgaaaattga tgaccgggag caggtactca aatacctcgg
     1741 actggcgggt aaaagcatta ataacctgga tcagattatt catgaaatcc tcacctattc
     1801 caggaatgcc cgtaccgaaa ttcaggaaga agaattcgaa ttaaaggatg tggtaaagga
     1861 aatattcgac ggactacgct tttccgccgg tccgggcttt tcgtttcagg aggagtattc
     1921 cggtgaaacc aaaatctatg cggataaaaa ccggctcagt accgtgcttc gtaatatcat
     1981 cagtaactct gttaaatata aaaaaaagga aattgaaaat ccctttgttt tcctgcgttt
     2041 tgaaaacaac aaccatcact gttcattcag cataacagat aatggagagg gcatacccct
     2101 ggaatgccag ccccgggtat ttgaaatgtt ttaccgtggt acttcaagcg ccaccggcac
     2161 cggactcggt ctttatatct gtaaagaaat gctggataaa atggatgtcc ttttcgccat
     2221 ttcttcaaaa ccgggagaag gcacgacttt taacatacag ctcaaaaaaa tcgcaacctc
     2281 attagtcaaa gaaaaaaaag caccggcatt aaatcccgca aaacaatccg gatcccccac
     2341 cgcctcctta aaattaaacg agcagttaaa tagtattcaa ggcctcagct cttttaacca
     2401 ggcgggttag tacctcatac ccgactcccg actcgtcctc cgtagcttca gcgaaggagg
     2461 atcttgctct tcccactatt aatacgtacc ttacttctcc gaatccttaa ccattctatg
     2521 tcccgccgcc tgatcccgat attcctgatc ctgacctgtt tatgcgcagc tgtaaacgcc
     2581 cagcctgccc ggcccgattt taacccgata gacctgcgcg cccgcagtat ccgctacgat
     2641 gatgatatcg tcaaactggt acaaaacctg accagtccct attccgataa attatcaaaa
     2701 gtccgctcta tttttatctg gatcaccgat catatccggt acgactggaa attctttaat
     2761 aaagacgagg agattgaaat ccccgaatgt aaatccggac aggattgtga tgaagtgctc
     2821 gctaattggg aaaaaaaata cctggcgaat attctccgga aaggaaaagc aatcagcgat
     2881 ggctatgccc ggctctttac ccggatgtgt gatatcgccg ggctggaagc agccacaatc
     2941 aacggacata ccaagaccaa actctaccag atcggcaatg ctggtcccgt aaaccatagc
     3001 tggaacgtga tctggctcga taccacctgg catttactcg atgccacctg gtcggcaggc
     3061 tataccaccg aagatgaaaa caccggcaaa ctcaatggtt ttgtcaaaca gtacaatgac
     3121 tattatttcc tgacaccttt tgccgaattt tcccgcaacc atttcccgca atacacccgc
     3181 tgggtattaa aacctggtta taccaaggac aagtttgctg ccaatcccta ttttgatccg
     3241 gatattctga gcaagatcag attgcattca ccacagacgg gaatgatcaa cgggaaaaaa
     3301 ggcgatacca ttcattttaa atttgaatac accggcacga ttgagaagtt gcaggtgaat
     3361 acgaatctct atcgcaacgc ggaagtctgg ggctgggaac aaataaataa acgcaaaaaa
     3421 atatgggtgc aggatacctt cgccctgaaa agacaacgct atacgccatt tataaaaacc
     3481 ggcaatctct atgaatttga ttatgtgatt ccggagaact cgctctatta cctggatatt
     3541 cttttcg
//
LOCUS       DAOPMW010000366         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31832_length_3522_cov_7.142486, whole genome shotgun sequence.
ACCESSION   DAOPMW010000366 DAOPMW010000000
VERSION     DAOPMW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31832_length_3522_cov_7.142486"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLU11_13630"
     CDS             <1..1073
                     /locus_tag="PLU11_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPN60221.1"
                     /translation="EGCQGSYLQRAMEAMQKNGGVALRQYPYNDQDCERAPNSSDVQM
                     GRQNVIHGFTRITNGDNINEISVRGIKEHLAKDAPVAIGMMVGQSFMQDMMGKELWQP
                     AGMDASQAGMGGHAMCVIGYDDRKFGGAFQIMNSWGPDWGKDGVAWVRYGDFRTYVRE
                     AYGIDPLPKRSAVSNIPLECSIGLVNNANKEYIQLRSSGGNTFQTVSPIALGTRFKME
                     IKNETECYIYIFGQEVDGKSFVLFPYLKQGESVSKHSPYCGITGYRLFPKAQSLEADS
                     IGTRDFMAIVVSKDELDYNQVNQAISNSSQPGYAGKVNEALRNIQIRSARYNNTNDGT
                     IYFKVDANDNKAVAMIVSFDKK"
     gene            1082..1585
                     /locus_tag="PLU11_13635"
     CDS             1082..1585
                     /locus_tag="PLU11_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HPN60222.1"
                     /translation="MNPHRSAFVRLIKHPLKFRLFLLMKLPAAFIAGVKVREIDEHRA
                     VVTVPFKWLTQNPFRSTYFASLSMAAEMSTGVLALAELHQRQPAVSMLVVKVESAYFK
                     KATDRSSFVCEDGELFRKAIDETIETGEARTVSARSVGTNKAGEKVAEFWITWSFKVR
                     STRYEVR"
     gene            1669..3072
                     /locus_tag="PLU11_13640"
     CDS             1669..3072
                     /locus_tag="PLU11_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN60223.1"
                     /translation="MKISFHGAARTVTGSKHVITLKNGKKLLLDCGMFQGLGKDTDML
                     NRNFGFEPEEIEVMILSHAHIDHSGLIPKLIKDGFRGKIFCTHGTSELAGVLLEDSAE
                     IQEDDVKYTNKRRAANGLPYLKPLYTVDDAKKAVDHLIEADYGKWFDVMEGVQAMFTD
                     AGHIIGSACVHLKIHESGKEIRITFSGDIGRYRDAILKSPEDFPQADYIIMESTYGNS
                     LHDTHVTTPDNLLEWIEKACVQKKGKLILAAFSVGRTQEILFSLNQLELENRLPDVPY
                     FVDSPLSIEATEIMKKYPEYFNKTIQKIMKTDKDPFSFEGLKYVKSVEQSKLLNFQDG
                     PMVIISASGMADAGRVKHHISNNIENSHNTILMTGYCEPRSLGGRLLKGDKEVNIYGV
                     LHEVHAEIGAVRSMSAHGDYEDMSQWLSCQDPRQVTKLFLVHGEFDVQQDFKQRLIRK
                     GFLDVEIPEQHQEIGLG"
     gene            3159..3232
                     /locus_tag="PLU11_13645"
     tRNA            3159..3232
                     /locus_tag="PLU11_13645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3193..3195,aa:Met,seq:cat)
     gene            3338..>3522
                     /locus_tag="PLU11_13650"
     CDS             3338..>3522
                     /locus_tag="PLU11_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60224.1"
                     /translation="MIKLPNGCSCSELSVFPRNWKTKNAKTSLKWYIKYRFYDPRFKD
                     PKQIVLKGMNQFNALFE"
BASE COUNT          980 a          718 c          949 g          875 t
ORIGIN      
        1 tggaaggttg ccagggctcc tacctgcaac gggccatgga agccatgcag aaaaacggcg
       61 gggtggccct tcgtcagtat ccctataatg accaggattg tgaacgggca cccaattctt
      121 ctgacgttca aatgggccgt caaaatgtaa tacatgggtt tacccggatc actaatgggg
      181 ataatatcaa tgagatcagt gtacggggaa tcaaagagca cctggccaag gacgcgccgg
      241 tagcgatcgg gatgatggtg gggcagagtt tcatgcagga tatgatgggc aaagagttgt
      301 ggcagcccgc cggaatggat gcctcccagg cggggatggg tggtcatgcc atgtgtgtga
      361 tcggctatga cgaccggaaa ttcggcgggg cttttcagat tatgaacagc tggggacccg
      421 actggggaaa ggatggggtg gcctgggtga ggtatggaga cttcaggacc tatgtacgcg
      481 aagcctatgg aatcgatccg cttcctaaac ggagtgccgt ttccaatatt ccattggaat
      541 gttctattgg tctggtcaat aatgctaaca aagaatatat tcaactacgc tcctccggag
      601 gcaatacgtt tcagacggta tcaccgattg cgttgggcac ccggttcaaa atggaaatca
      661 aaaacgagac cgagtgttat atctatatct tcggacagga agtggatgga aaaagttttg
      721 tgttgtttcc ttacctgaaa cagggggaat cggtttccaa gcactcccca tactgtggca
      781 ttaccgggta ccgtttattc cccaaagcgc agtcgctgga agcagacagt atcggaacac
      841 gcgactttat ggccatcgtg gtcagtaagg atgaactgga ttataaccag gtgaaccagg
      901 cgatcagtaa cagttcgcaa cctggctatg cgggtaaagt gaacgaggcg ttgcgaaata
      961 tccagatccg ttcagcgaga tataacaata ccaatgatgg cacgatttat ttcaaagtgg
     1021 acgccaatga caacaaggcg gtggccatga ttgtgtcatt tgataaaaag taaaggacgt
     1081 catgaatccg caccggtctg cctttgtccg tttgattaaa cacccgctga agttccggtt
     1141 gtttctgctc atgaaactac ctgcggcctt tattgccggg gtaaaagtga gggagatcga
     1201 tgagcaccgg gcggtggtca cagtaccgtt taagtggtta acgcaaaacc cattccggtc
     1261 cacctatttt gccagcctga gtatggccgc ggaaatgagt accggggtgc tggcccttgc
     1321 tgagttacat cagcgacagc ccgccgtttc gatgctggta gtaaaagtgg aatcggcgta
     1381 ttttaagaag gcaacggacc gatcttcttt tgtttgtgag gatggtgaat tgttccggaa
     1441 ggcgattgat gaaacaatag agactggaga agcccggacc gtgagcgccc gttctgttgg
     1501 tacgaataaa gcgggggaga aagtagctga attctggatc acatggtcat tcaaagtacg
     1561 aagtacgagg tatgaagtac gctgacgtaa attgaacata tagttgtaag gaggggctgt
     1621 ttcctaacag cgaacaccta acagctaaca cctattaaaa gaagaattat gaaaatatcc
     1681 ttccacggcg cagcacgcac agtaactggt tccaagcatg tgattacgtt aaagaatgga
     1741 aagaagctcc tgcttgactg cgggatgttt cagggattgg gaaaggatac cgatatgctg
     1801 aaccggaatt ttggttttga gccggaagaa attgaggtga tgattctttc gcatgcgcat
     1861 attgatcaca gtgggcttat ccctaaactg atcaaagacg gattccgggg taagatattc
     1921 tgtacccatg gtaccagtga actggcaggc gtgttgctgg aggattcagc agaaatccag
     1981 gaagatgatg tgaagtatac gaataagcgc agggcggcca acggactgcc atacctgaaa
     2041 cccctgtata cagtagatga tgcaaaaaaa gcagtggatc atcttattga agcagattat
     2101 ggaaaatggt ttgatgtgat ggaaggggtg caggcgatgt ttacagatgc cggacatatt
     2161 atcggcagtg cctgtgtgca tctgaagatt catgaaagcg gaaaagagat acggattact
     2221 ttcagcgggg atattggtcg ttaccgggat gcgatattaa aatctccgga ggattttcct
     2281 caggccgatt atatcattat ggaatccact tatggaaaca gtctgcatga tacccatgta
     2341 accacacccg ataatctgct ggaatggatt gagaaagcct gtgtgcagaa aaagggcaaa
     2401 ctcatattgg ccgctttcag tgtggggcgg acccaggaga ttctgttttc cctgaaccag
     2461 ctggaactgg aaaaccgctt gccggatgtg ccttacttcg tagatagtcc gttaagtatt
     2521 gaagccacgg agatcatgaa gaaatatccg gagtatttca ataaaacgat acagaagatc
     2581 atgaagaccg ataaggaccc gttttccttt gaaggattga agtatgtgaa gtcggtcgag
     2641 caatccaagc tgctcaattt ccaggatggt ccgatggtga tcatttctgc cagtggtatg
     2701 gcggatgccg gccgggtgaa gcaccatatc agtaataata tcgagaacag tcacaatacg
     2761 atcctgatga cgggttattg tgaaccccgt tcattgggtg gacggttatt gaaaggcgat
     2821 aaggaagtga atatttacgg ggtattgcat gaagtccatg ccgaaatcgg cgccgtccgc
     2881 agtatgagtg cccatgggga ttatgaggat atgagccagt ggctttcctg tcaggatcct
     2941 cgccaggtga ctaaattatt tctggtccac ggagagtttg acgtgcagca ggattttaag
     3001 caacggttga tccgcaaagg cttcctggat gtggagattc cggagcagca tcaggaaatc
     3061 gggcttgggt gacaaggagt tagccggaaa atctcttaaa aaaaggttcc aatttcattt
     3121 cgccaatttc cctaaaatcg ctatttttgc cgcccgttgc cggggtagct cagctggtta
     3181 gagcatcgga ttcataaccc gaaggtcggc agttcaagtc tgctctccgg tacttagcct
     3241 aaatctttta caataaaagg tttaggcttt tttgtgccct gtcaatagtt gtagtatttc
     3301 ctaacagtta ccaaaatggt taccaaaaac tacaacaatg ataaaactac ctaatggctg
     3361 ctcctgcagt gaattatctg tctttccccg gaactggaaa acaaaaaatg ccaagacctc
     3421 ccttaaatgg tacatcaagt acaggtttta tgatcccaga tttaaggatc caaagcaaat
     3481 cgttttaaaa gggatgaatc aatttaatgc cttgtttgaa ag
//
LOCUS       DAOPMW010000367         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_31924_length_3515_cov_6.532370, whole genome shotgun sequence.
ACCESSION   DAOPMW010000367 DAOPMW010000000
VERSION     DAOPMW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31924_length_3515_cov_6.532370"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLU11_13655"
     CDS             <1..1181
                     /locus_tag="PLU11_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPN60225.1"
                     /translation="LAFKIMTDPFVGRLAFFRCYSGHLDAGSYVLNVRSGKKERISRI
                     MKMFANKQNPIDFIEAGDIGAAVGFKEIKTGDTLCDENHPITLENMFIPEPVIAVAVE
                     PKTQADVDKMGMAIAKLVEEDPTLRVNTDEDTGQTILRGMGELHLEIIIDRMRREFKV
                     EVNQGAPQVAYKEAFQATIEHRETLKKQTGGRGKFADIQFSLGPVDAEWKTENPDKHY
                     QFVNDLFGGSIPREFVPAIQKGFEQAMTNGVLANYPVDNLKIRVFDGSFHAVDSDSMS
                     FELCAKQGFREAARKAKPVLLEPIMKVEVITPAQYMGDVTGDLNRRRGMLEGMDTRAG
                     AEVIKAKVPLSEMFGYVTQLRSLSSGRASSTMEFSHYSPAPNNIAEEVIAKVKGKVNA
                     "
     gene            complement(1360..2253)
                     /locus_tag="PLU11_13660"
     CDS             complement(1360..2253)
                     /locus_tag="PLU11_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPN60226.1"
                     /translation="MNQDRSRFRWLKLALKIVVSGVCIWYISGKVDVEQAGKLLLQAD
                     WFYGLPALVLFVLSKLVSAIRLQVYFSNIQLPITARENTRLYWLGMFYNLFLPGAISG
                     DIYKVVLLSKRFNQPFKKITGAVILDRFSGLLGLGILLAFLACIVSLPAAVPILISLA
                     TLLSITLFYFFLRRYFPDFKKGFYHTLLLGIAVQALQIACVYMIMKSLGITINEPAYL
                     FIFLVSSAASVLPVTIGGLGIRELVFLEGSVWFQNSTEKAVLISLIFYLLTLFTSVWG
                     AVWLFKSPINEKGPSDEPLIS"
     gene            2288..>3515
                     /locus_tag="PLU11_13665"
     CDS             2288..>3515
                     /locus_tag="PLU11_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPN60227.1"
                     /translation="MTKRILTFLLIFLTTTITAQELTLRAGLRITQSQKIKKGIYLLD
                     APADTSHSLILIEGNNLIIDFNQAELKGSNGKMPDAFFGVALLIRNSKHVTIRNLKAN
                     GYKVALLARNVEQLTLENCDFSYNYRQHLNSTQEKEDISDWMSYHHNEKDEWLRYGAA
                     MYLRNCDQAVIRHCRVTGGQNALMMTESDHGKIYNNDFSFNSGIGIGIYRSSANEFSY
                     NRLNFNVRGYSYGVYNRGQDSAGFLVYEQSHYNIFYKNSATHSGDGFFLWAGQTTMDT
                     GEGGCNDNVIHENDFSYAPTNGVEVTFSRVAVSGNLIYECDHGIWGGYSFNSKFINNQ
                     FRDNRIAIAIEHGVHNEISYNTFSKDKEAIRLWSRKNQPSDWGYARFRDTRSADYIIA
                     GNSFNHHPLGLNISGTD"
BASE COUNT         1006 a          765 c          862 g          882 t
ORIGIN      
        1 ccctggcttt caaaatcatg actgacccgt ttgtaggtcg tctggccttc ttccgttgct
       61 actccggaca cctggatgct ggttcttatg tgctgaacgt aagaagtggt aagaaagagc
      121 ggatcagccg gatcatgaaa atgtttgcca acaagcagaa cccgatcgac ttcatcgaag
      181 ccggtgatat cggtgccgct gttggtttca aagagatcaa aaccggggat accctttgcg
      241 atgagaacca tccgatcacc ctggagaata tgtttatccc tgagcctgta atcgctgttg
      301 cggtagaacc taaaacacag gctgacgtgg acaaaatggg tatggcgatt gccaaactcg
      361 tggaagaaga tcctacactg cgtgtaaata ccgatgaaga taccggtcag accatccttc
      421 gtggaatggg tgagttgcac ctggagatca tcattgaccg tatgcgtcgt gagtttaaag
      481 ttgaagtaaa ccagggtgca ccccaggtag cttataaaga agccttccag gccactattg
      541 aacaccgtga gacgcttaaa aagcagactg gtggtcgtgg taaattcgcc gatatccagt
      601 tcagtctcgg accggtggat gcagaatgga agaccgaaaa tcctgacaag cattaccagt
      661 ttgtaaacga tctgtttggt ggatctattc cccgtgaatt tgttccggct attcagaaag
      721 gttttgagca ggcgatgacc aatggggtgc tggccaacta tcccgtggac aacctgaaga
      781 tccgagtatt cgacggtagc ttccacgctg ttgactctga ctctatgtct ttcgaacttt
      841 gcgccaagca aggtttccgt gaagcggccc gcaaagcgaa acccgttctg ctggagccca
      901 tcatgaaagt ggaagtcatc acacccgccc agtacatggg agatgtgaca ggtgacctga
      961 accgccgtcg tggtatgctg gaaggtatgg atacccgtgc cggagcagag gtaatcaagg
     1021 ccaaagtgcc cctgagcgaa atgtttggtt atgtaaccca actgcgttct ctgagttctg
     1081 gccgtgcttc ttcaaccatg gagttctcgc actattctcc cgcccccaac aatatcgctg
     1141 aagaagtgat tgcgaaggtg aaagggaaag tgaacgcctg attcatctga tttttctata
     1201 aaaaaccccg gaattttccg gggttttttc atttacttta cctgaatcaa tcgtgttttt
     1261 acgaaatggc attgtgtttg ctataaaata gcggaattcc ccgtttttta gccaatacct
     1321 gtttaaaggc ctgattcaaa gaagtatgaa gaactccgtt caagagatta agggctcatc
     1381 actgggccct ttttcattta tagggctttt aaacagccag accgctcccc acactgaagt
     1441 aaacaaggtc aacagataaa aaatcaggct gataagcacc gctttctctg tactgttttg
     1501 aaaccagaca gacccctcca ggaaaaccaa ttcccttata cccaatcccc caatagtcac
     1561 aggcaataca gacgccgccg aagaaaccag aaagataaac aggtaagccg gttcgtttat
     1621 ggtgattccc aacgatttca tgatcatgta aacacaggca atttgcagtg cctgaacggc
     1681 tatacccagg agcagggtgt ggtagaatcc ttttttaaaa tccgggaaat atcggcgcag
     1741 aaaaaaataa aacagtgtta tagataacag ggtggccaaa ctgatcagta tgggaacagc
     1801 ggccggtaat gagacaatgc aggccaaaaa ggccaacagg atgcccagac cgagcaggcc
     1861 actgaaccga tccagaatca ccgcaccggt gatcttttta aagggttggt tgaatctttt
     1921 ggatagcagg actactttgt aaatatcccc gctgatggct cccggtaaaa aaagattgta
     1981 aaacataccc agccagtaaa gccgggtatt ttcacgggcg gtgatgggta gctgtatatt
     2041 gctgaaatat acctgaaggc ggatggcgga gaccagtttg gaaagaacaa acaaaaccag
     2101 tgcaggcaac ccgtaaaacc aatctgcctg caggagcaat ttcccggcct gctccacatc
     2161 aacttttcct gaaatatacc agatacagac tccggacacc actattttaa gtgctaactt
     2221 gagccagcgg aaacgggatc gatcctgatt cataatggtt gatatgtttg caagtaaact
     2281 taaaatgatg acaaaacgaa ttctgacgtt tttgctgatt tttttaacga caacgattac
     2341 agctcaggaa ctgacgctgc gggcaggttt gcgcatcacc caaagtcaga aaattaaaaa
     2401 agggatttat ctgcttgacg cacccgctga taccagccat tcattaattc tcattgaagg
     2461 aaataacctg atcatcgatt tcaatcaggc tgaactgaaa gggagtaatg ggaaaatgcc
     2521 ggatgctttt tttggtgtgg ccctgctgat ccgcaatagt aagcatgtaa ccatccgcaa
     2581 tctgaaagcc aatggctata aagtggcgtt actggcccgg aatgtggaac agctgaccct
     2641 ggagaattgt gacttcagtt ataattaccg gcaacatctt aacagcaccc aggagaaaga
     2701 agatatctcc gactggatga gctatcatca caatgaaaaa gatgaatggc tgcggtatgg
     2761 ggcggcgatg tatctgcgta attgtgatca ggccgttatc aggcattgcc gggtaacggg
     2821 tgggcagaat gcgttgatga tgacggagag tgatcatggt aaaatttata acaatgattt
     2881 ttcctttaat tcaggtatcg gtatcgggat atacaggagt agtgccaatg agttttccta
     2941 caaccggctg aattttaatg taaggggata cagttatggt gtttataaca ggggacagga
     3001 cagtgccggg ttcctggttt atgagcaaag ccactacaat attttttata agaattcagc
     3061 tacccacagt ggcgatggtt tttttctctg ggccggtcag accacgatgg atacaggaga
     3121 aggtggttgt aatgataatg tgatccatga gaatgatttt tcgtacgccc ctaccaatgg
     3181 ggtggaggtg acattcagcc gggtggccgt atccggtaat ttgatttatg aatgtgatca
     3241 tgggatctgg ggcggttaca gttttaattc aaaattcatc aataatcaat tcagggacaa
     3301 ccggattgcc attgcgattg agcatggtgt acataacgaa atttcctata atactttttc
     3361 aaaggacaag gaagcaatca ggttatggtc ccggaagaac cagccttccg attggggtta
     3421 tgcccggttc agggacacca gaagtgcgga ttatatcatt gccggtaata gcttcaatca
     3481 ccatccttta gggctaaaca tcagcgggac agaca
//
LOCUS       DAOPMW010000368         3508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_32017_length_3508_cov_6.978569, whole genome shotgun sequence.
ACCESSION   DAOPMW010000368 DAOPMW010000000
VERSION     DAOPMW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32017_length_3508_cov_6.978569"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLU11_13670"
     CDS             <1..198
                     /locus_tag="PLU11_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60228.1"
                     /translation="LDLDLVGFFSRFGFGRFFFQYWILVFFGSGSFGLLLDWIGLVFR
                     QIRICRFSVGFGSQTVFSKSS"
     gene            complement(618..1958)
                     /locus_tag="PLU11_13675"
     CDS             complement(618..1958)
                     /locus_tag="PLU11_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPN60229.1"
                     /translation="MMDIKNNILETIGNTPLIRLNKITREIPATVLAKVDYFNPGNSI
                     KDRMALKMVEVAEQEGKLKPGGTIIEGTSGNTGMGLALAAVVKGYKCIFVTTDKQSKE
                     KADILKAVGAEVIVCPTNVLPEDPRSYYSVAARLAREVPNSFHMNQYDNLANRLAHYE
                     STGPEIWNQTDGKITHLVGTPGTAQYLKEKNPAIQIWAIDVYGSLLTKYFRTGEIDMN
                     EVHPYISEGFGEDFVPKNYDMSVIDYFEQVTDKDGAVMARRLAKEEGLFCGYSAGSCI
                     QGLMQLKNKLTKDDLVVCIFHDHGSRYVGKIYNDQWMIERGFLDVKTFKDVVSARGTK
                     RLITVEPSQTVASAVELMKKYDIEQIPVFNETGPVGAISEGGLFQKIFDNPEIKNATV
                     AEVLEPTFPLVAFDTPVERLSHLINKENGAVLAKDEAGNYHIVTKYDVIQSLGN"
     gene            complement(2081..2590)
                     /locus_tag="PLU11_13680"
     CDS             complement(2081..2590)
                     /locus_tag="PLU11_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60230.1"
                     /translation="MNTQQSMEERLWEYIDGTATKEDRLRIETLLQSQAEWKEKYSEL
                     LEINQLLHSSELDAPSLRFSKNIMEEITRLQIAPATKSYINKKIIWGIGIFFITLIVA
                     ILGYGFSQMDWTTTGKSSFPEQLSKIDTSRFFNNDLINVFMMINVLLGLVLLDNYFSN
                     KRKQFRKEA"
     gene            complement(2597..3196)
                     /locus_tag="PLU11_13685"
     CDS             complement(2597..3196)
                     /locus_tag="PLU11_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN60231.1"
                     /translation="MLSYYQMSAGQNDNELISKVLRGDHLAYAGLVNRYQNYVFTLTL
                     RLVKNREEAEEVAQDVFIKAYKYLADFRGDSKFSTWLYTIVNNTCISFLRKKKLIIHS
                     LDDERVFAKADNQDAGFRADGVEQKSRHVMVGEAISRLKPDDASIITLFYKNEQSLEE
                     IAQVLGLEVNTAKVRLHRARARLKELMETHYAHEVKDLN"
BASE COUNT          862 a          780 c          716 g         1150 t
ORIGIN      
        1 ttggatctgg atcttgttgg ttttttctct aggtttggat ttggacggtt tttctttcag
       61 tactggattt tggttttttt cggatctgga tcttttggtc ttcttttgga ttggattggt
      121 ttggtttttc gtcagataag gatctgtcgg ttttcagtcg ggtttggatc tcaaacggta
      181 ttttcaaaaa gctcttaaag taaaaaaaag aagttgaccg atactggatt tttaattgtc
      241 tggtttttct aaggattggg ttttgtcagt ttttggacgg ttcttctaat tttggatatt
      301 ggactttgac aaaagattga tacgatatca atcaacttct gatacaaaag taattggcat
      361 ttcattcaca aaacagagca aaataacctc ttttttttac gacatttttt accgttttta
      421 tcgtaaagtc atcatactta tccttcgcat tttcacaagt aatccacacg taattatacg
      481 ataaaaagac cccgtcaatt ggttgacggg gtctttttat tttttaaaat atttgtagtc
      541 agtcttttgt gaagcttact taaagcttgg tctggcgcat tcattttatt gaaaaattgc
      601 ctttatcatt tattgtgtca gttcccgagt gactgaatca cgtcatattt cgttacaata
      661 tggtagttcc cggcctcatc ttttgccagt acggcaccgt tctctttgtt aatcaggtga
      721 cttaagcgtt ctaccggtgt atcaaaagcc actaatggga aggttggttc taaaacttcg
      781 gccaccgttg cgttttttat ttcagggtta tcgaaaattt tctgaaataa accaccttcg
      841 ctgatagccc cgacggggcc ggtttcattg aacaccggta tttgttcgat atcatatttc
      901 ttcatcagtt ctaccgccga ggcaactgtt tgtgatggct caacagtgat cagtcttttc
      961 gtacctctcg cactcactac atctttgaat gttttaacat cgaggaatcc acgctcaatc
     1021 atccactgat cattgtagat cttaccaaca taacggcttc catgatcatg gaaaatgcaa
     1081 acgaccaggt cgtctttggt taatttgttt ttcagctgca ttaatccttg tatacaactg
     1141 ccggcactat agccgcagaa caatccttct tccttggcga gtcttctggc catcacggct
     1201 ccgtctttat ccgttacctg ttcgaaataa tcgatcacgc tcatatcata attcttcggg
     1261 acaaaatcct ctccgaatcc ttcactgata tatggatgta cttcattcat atcgatctca
     1321 cccgtccgga aatatttggt gagcagtgaa ccataaacat cgatggccca tatctggatt
     1381 gcggggttct tttctttcag gtattgcgcc gtaccggggg tacccaccag gtgggtgatc
     1441 ttaccatccg tttgattcca gatctcaggc ccggttgatt cataatgtgc cagccggttc
     1501 gccaggttat catattggtt catgtgaaag gaattgggca cttccctggc cagccgggcg
     1561 gctacgctat aatagctgcg gggatcttcg ggtaacacat tggtgggaca aacaatcact
     1621 tctgccccca ccgctttcaa tatatctgct ttttccttgg attgtttatc cgtggtgaca
     1681 aatatgcatt tgtatccttt aaccactgcc gccagggcca gtcccatacc ggtattgcca
     1741 ctggtgcctt caatgattgt accgccaggt ttcagtttgc cttcctgttc ggccacttcg
     1801 accattttca gggccatccg gtctttaatg gaattaccgg gattgaaata atccacttta
     1861 gccagtacgg tggctggtat ttcacgggtg attttattta accggatcag gggggtgttt
     1921 ccgatcgttt cgagtatatt attttttata tccatcattg gttttttctg atggcaaagg
     1981 taggttttta ggcctgtatc attaacagct gttagttaaa acaaataaaa agcccctcct
     2041 ggtaccagga ggggttgccg gacttaatga acagttgtca tcaagcttct tttctgaatt
     2101 gttttcgttt attgctaaag tagttgtcca gcaaaaccaa tcccaaaagc acattgatca
     2161 tcataaaaac attaatcagg tcattattga aaaaccttga agtatctatt ttactgagtt
     2221 gttcagggaa actgctcttg ccggtggtgg tccaatccat ctggctgaaa ccatatccca
     2281 agatggctac aattaaagtg ataaaaaaaa tgccgatgcc ccagatgatc tttttgttta
     2341 tataggattt ggtagccggt gcaatctgca accgggtgat ttcctccata atatttttgc
     2401 tgaaccgtag ggagggggca tccagctcag acgaatgcag cagttgattt atttccagca
     2461 gttcgctgta tttttctttc cattcggcct gtgattgcaa tagtgtttca atccgcagcc
     2521 ggtcctcttt agttgcggtg ccatcaatat attcccacaa ccgttcttcc atactctgtt
     2581 gcgtattcat aatggttcaa tttaagtctt ttacttcatg tgcataatgt gtttccatca
     2641 gttctttcaa cctggccctt gcccggtgta gccgcacttt ggctgtattc acttccagtc
     2701 ccaatacctg ggcaatttct tccaggcttt gttcattttt ataaaacagg gtgataatcg
     2761 atgcatcatc cggtttgagc cggctgatcg cttccccgac catcacatgt cttgatttct
     2821 gctccacccc gtctgccctg aatcccgcat cctggttatc tgcctttgca aaaacccgct
     2881 catcatccag ggaatgaatg atcagtttct tctttcgcag aaaactgata caggtattat
     2941 tcacgatcgt atagagccag gtgctgaatt ttgaatcacc cctgaaatcc gccaggtatt
     3001 tataggcctt tataaatacg tcctgggcca cttcctctgc ttcctcccgg ttcttcacca
     3061 ggcgcagcgt aagcgtaaaa acatagttct ggtaacggtt caccaaaccg gcataagcca
     3121 ggtggtctcc ccgcaaaact ttgctaatga gttcattatc attttgtccg gctgacatct
     3181 ggtaataaga cagcaacagg ggtgttcagg ttacaatgtt ggatattaat atataatgcc
     3241 cccgtttgaa atagaaagtt acggaaagac tgtaacctgt cgtggccggc tgtggtctga
     3301 tattttgtaa actgttacaa aaaacttcta ccggcctgta accggaacaa aacggaagga
     3361 gtcatagcta acaagcgatt aaaaaaaaca taaaacttac aactatgaac ggagcagaag
     3421 tattagtacc cgtagtgatg ttcgcaggcg gatttgccat gatctttggt attgcctacc
     3481 tcaaaacccg ccagaacctg gccatgat
//
LOCUS       DAOPMW010000369         3483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_32330_length_3483_cov_7.375146, whole genome shotgun sequence.
ACCESSION   DAOPMW010000369 DAOPMW010000000
VERSION     DAOPMW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32330_length_3483_cov_7.375146"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1000
                     /locus_tag="PLU11_13690"
     CDS             113..1000
                     /locus_tag="PLU11_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN60232.1"
                     /translation="MKKDWLILMLLVFSTGQAYAQAADTVYKPVEFPEGYSTKLNVVY
                     AKVKDWEGKMDLYLAPKEKGLSPVVINIHGGGWNHGVKESQTGFSTFFKAGFAVANIE
                     YRLTGQATAPAAVEDTRCALIYLIKNAKELNIDVNKIVIMGGSAGGHLALMGGLLGND
                     HRFDGNCPGVENIKVAAIIDKYGITDVWDWGFGKNLTSKSARNWLGEKANDQSFAASV
                     SPITYVTKESPPVFIVHGDADPTVPYQQSVDLHKKLKEAGVKTEFITVPGGLHGKFEK
                     TVNSDLNKAIIKFIMEVFN"
     gene            complement(1027..2793)
                     /locus_tag="PLU11_13695"
     CDS             complement(1027..2793)
                     /locus_tag="PLU11_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPN60233.1"
                     /translation="MKKVLFTAGFLLQVFLIPSTTRSQQLNNSLVQVADSNLILPPAW
                     AFGVLYGGYTNQHETIKRVDSIMAHQYPIDAYWIDSWFWSHADKGIGPHKYIDFVADT
                     IGYPDRKKMWGYLQQYGIKGGFWTWDCILETGNEKAFHDFKKQGYFSSVYTNKNSWHN
                     KGTSTAMFQEKAEHPGTACGNIDFDNPAAVAYFKQQMKRFFSEGADFIKLDRTSKIST
                     CKAMFEMSQEFGLETKGRGFILSHTGGMETEEYKRYPTKWTDDTRSDWNIEQPLLKFN
                     PWVPAVAFKENIAMFTDPGNPSSRIPFLTNDLGGFDMGFTAKPEEELYIRWLQFSMFN
                     PITEVFSQPENPTANLAWNYSERADSIFRFYAHLRMKLFPYIYSYAHRSRLDGKTMIG
                     KLPGQLYEYLFGDEILVAPVYVRGAVNRQVFLPAGNWFNYWTGEKLAGNRTHIVVAPI
                     QQIPLFVKEGSIIPYRNYASSIEKGNNKELFLDIYSDKTGSFTLVEDDGLSNEYLEGK
                     YTATRIELKKSGITILPSTGSFNGQLLKRNWTIRMYNSKKPKKIWLNGSTVKFSYIPE
                     KKLLTIETGEISTSELQHIRIK"
     gene            complement(2790..>3483)
                     /locus_tag="PLU11_13700"
     CDS             complement(2790..>3483)
                     /locus_tag="PLU11_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPN60234.1"
                     /translation="GGHEMLVPSALTLISADHIKNTGKLNILAIGDSNGAAANGWPNQ
                     MQKLLPYSTILNSCISGNTIGFDNLGQEKLNTLKNINRYLDEAYRQLGPSGQLDYILI
                     GLGTNDTKTIFNEQQKEIPELLAKLLEQIKNHLQVNNKKSPAIILLLPPPVEEQKADQ
                     AKYGGSAGRLQINQPLFKMVAASHQAAIIDLSILRAELTDELTIDGIHLTVKAQFRVA
                     AIILEYINKGKK"
BASE COUNT          912 a          745 c          799 g         1027 t
ORIGIN      
        1 ggggtatata acacgtgaca aattgttatt gccgattcct caacgggaaa ttgataacaa
       61 cacacaactg gtcatcccgc agaatcctgg ttattaatta taaataataa aaatgaaaaa
      121 ggactggctt atactgatgt tactggtttt ttcaaccgga caggcttatg ctcaagcggc
      181 ggatacggtc tataaaccgg tagagtttcc cgaagggtac agtacgaaat tgaatgtggt
      241 ctatgccaag gtgaaagact gggaagggaa gatggatctc tacctggcac caaaggagaa
      301 aggactgtcc ccggtagtga tcaatattca tggaggagga tggaatcatg gagtaaaaga
      361 atcacagacc ggtttcagta ctttctttaa ggcaggcttt gccgtggcca atatcgaata
      421 ccggctaact ggccaggcta cggcacccgc tgcagtggag gatacccgct gtgccctgat
      481 ctacctgatt aaaaatgcaa aagagttgaa tattgatgta aacaaaattg tgatcatggg
      541 tggcagtgcc ggtggtcacc tggctttgat gggtggctta cttggtaatg atcaccgttt
      601 tgacggcaac tgtccgggtg tggagaatat aaaagtggcg gccataattg ataaatacgg
      661 gatcactgat gtatgggact ggggatttgg aaagaatctt accagtaaat ccgcgcggaa
      721 ttggttaggg gagaaggcca atgatcagtc atttgcagca tccgtttcgc cgataacata
      781 tgtaacaaaa gagagtcctc cggtttttat tgtacatggg gatgcagatc cgaccgttcc
      841 ttatcagcag tcggtggacc tgcataagaa attaaaagaa gcaggcgtga agaccgaatt
      901 tatcaccgtt ccaggcggcc tccacggaaa atttgaaaaa acagtaaaca gcgatctgaa
      961 taaagcgatt attaagttta ttatggaggt gtttaactaa ccattctcca tacaacatgt
     1021 ccgggtttac ttaatccgga tatgttgtaa ttcgcttgtt gaaatctccc ctgtttcaat
     1081 tgtcagcagc ttcttttctg ggatgtagct gaatttaacc gtgctcccgt ttaaccaaat
     1141 tttttttggt tttttactgt tatacatccg gatcgtccaa ttccttttta atagctgacc
     1201 gttgaagctt cccgttgaag gaagaatggt tatacctgat tttttgagtt caatgcgtgt
     1261 agctgtatat ttcccttcca ggtattcatt gcttagtccg tcatcttcca ccagtgtaaa
     1321 actgcctgtc ttgtcagaat agatatccag aaacaattcc ttattgttgc ctttctcaat
     1381 tgaagaggca taattccgat aaggaataat tgatccctct tttacaaaga gcgggatctg
     1441 ttgaatcgga gcaacaacta tatgcgtcct gtttcccgcc agtttttcac cggtccagta
     1501 attgaaccag ttacctgcag gcaggaatac ctgccggttt actgctccac gcacgtacac
     1561 cggggctacc aggatttcat caccaaacaa atattcatac aactggccgg gtaatttacc
     1621 gatcattgtt ttgccgtcca gccggcttcg gtgcgcataa ctatagatgt aaggaaataa
     1681 cttcattcgt aaatgtgcat agaaccggaa aatgctatcg gctctttcgg aataattcca
     1741 ggccaggttg gcagttggat tttccggttg agaaaatact tcagtaattg gattaaacat
     1801 ggagaattgc agccagcgga tatatagctc ttcttccggt tttgctgtga atcccatatc
     1861 aaatccaccc aggtcattgg tcagaaaagg aatccggctg cttggattgc cggggtcggt
     1921 aaacatagcg atattttcct tgaatgccac tgccggtacc caaggattga attttaataa
     1981 gggttgttct atgttccagt cactccttgt gtcgtctgtc cacttggtgg gatagcgttt
     2041 gtattcttct gtttccatgc cgccagtatg ggataagatg aatccccggc cctttgtttc
     2101 cagtccgaac tcctggctca tttcaaacat agctttacag gtactgatct ttgatgtacg
     2161 gtcgagtttg ataaagtcgg ctccttctga gaaaaatctt ttcatctgct gtttgaaata
     2221 agcgacagca gcagggttgt caaaatcaat attgccacag gctgttcctg ggtgttcggc
     2281 tttttcctgg aacatagccg tgctggtacc cttattatgc cagctgtttt tattggtata
     2341 aacggaagag aaatacccct gtttcttaaa atcatgaaat gctttttcgt taccggtttc
     2401 caggatacag tcccaggtcc agaaaccgcc tttaatgcca tattgctgaa ggtagcccca
     2461 catttttttc cggtccggat acccaatcgt atcagctaca aagtcgatgt atttatgcgg
     2521 accgatccct ttatctgcat ggctccagaa ccaggagtcg atccaatagg catcaatcgg
     2581 gtattgatgt gccatgatcg aatccacccg tttaatcgtt tcatgctgat tagtatatcc
     2641 accatataaa accccaaaag cccaggctgg aggcaggatc agattactgt cagcaacctg
     2701 tacaaggctg ttgtttagtt gctgtgacct tgtagtggaa ggaatcagga atacctgcaa
     2761 caggaagcct gctgtaaaaa gcactttctt cattttttcc ctttgtttat atattccaga
     2821 ataattgcgg caacacgaaa ctgggctttt actgtcaggt gaatcccgtc aatggtaagt
     2881 tcgtccgtta gttcagcacg cagtatggac aaatcaataa tggctgcctg gtggctggct
     2941 gccaccatct taaaaagggg ctggtttatt tgcagccgcc cggccgaacc gccatatttt
     3001 gcctgatccg ctttttgctc ttcaaccggc gggggcagca ataaaatgat agccgggctt
     3061 ttcttattat ttacttgcag gtggtttttt atttgctcaa gcagttttgc caaaagctcc
     3121 ggtatttctt tttgctgttc attgaatatg gttttggtat cattggtacc gaggccaatc
     3181 aggatataat ccagctgacc tgacggcccc agttgccggt aggcttcatc caggtaacgg
     3241 ttaatatttt tcagcgtgtt cagtttttcc tgtccgagat tgtcaaagcc aattgtgtta
     3301 cctgaaatac aggaattaag gatagtggaa taaggaagca gtttctgcat ctgattgggc
     3361 cagccattgg ctgccgcgcc atttgaatca ccaatggcca ggatattcag tttgccggta
     3421 ttctttatgt gatcagctga aatcaatgtc aatgcggaag gtaccagcat ttcatgacca
     3481 cct
//
LOCUS       DAOPMW010000370         3458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_32649_length_3458_cov_8.930356, whole genome shotgun sequence.
ACCESSION   DAOPMW010000370 DAOPMW010000000
VERSION     DAOPMW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32649_length_3458_cov_8.930356"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..860)
                     /gene="dcd"
                     /locus_tag="PLU11_13705"
     CDS             complement(324..860)
                     /gene="dcd"
                     /locus_tag="PLU11_13705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPN60235.1"
                     /translation="MILSDTRILEEMEKGTIKVVPYQRDCLGSNSYDVHLGKTLATYK
                     EHMIDAKKHNEIDYFEIPDEGIVLYPHIFYLGVTEEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVKVYKGMPIGQLIYFPVDGEIEIKYNQKKNSK
                     YSNQPDKPVESMMWKNKF"
     gene            911..1540
                     /locus_tag="PLU11_13710"
     CDS             911..1540
                     /locus_tag="PLU11_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPN60236.1"
                     /translation="MPVFFQQQINESTRLGVWKIEETEAFFRGNVPIHRDVTHPKKRL
                     QHLAGRFLLQYLFPDFPYEQIRIADTRKPYLPDERYHFSISHCGDYAAAIVSRTHRVG
                     VDVEETEDRILRIKDKFMSAKEQSLFIPDPFTPDYRLLTLLWSAKESVYKWYGAGEVD
                     FRRHILLSELDVTDNKLRCDFGLTGDRLKVEFRFFDGLVLSWVVDRFTS"
     gene            complement(1627..2823)
                     /locus_tag="PLU11_13715"
     CDS             complement(1627..2823)
                     /locus_tag="PLU11_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HPN60237.1"
                     /translation="MSTEIKCPNCGHQFEPNDAIRDQVEQELRAKIADWQKKKNEEFQ
                     QKMDEEKKRLQESLEGNLRKSIAADFETQLKMAEQNNKDYEEKLQQARKQQLEFLKKE
                     QELKNKEAELELSVQQQLQKEREKLSVEIRKLEEQRMSAKETEFQLQLKELQKQLDDQ
                     KKLADEMRRKAEQGSMQTQGEVQELLLEELLKTAFPFDLVSEVGKGVRGADCIQTVRN
                     NFGQECGKIIFESKRTKDFGNDWIEKLKADMRSQGADVAVIVSQALPKDMDRFGEKDG
                     VWICSFAEVKPLVQMIRYGIINVANAARSQENKGDKMHLLYKYLTSREFADQWSAIRE
                     GFMSMKLSIQKERDAMEKLWKAREKQLEKVMINATGIRGSIEGISGADVDLNLLGDGN
                     DDLVLD"
     gene            2997..>3458
                     /locus_tag="PLU11_13720"
     CDS             2997..>3458
                     /locus_tag="PLU11_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60238.1"
                     /translation="MSKSSFLIHPQQLFFSTESQTSNFYPACRLPRFLGICLFSILIF
                     SSCKKGDTPAAEELKLGTCAAVTPKAGMIQSGVADSFVYRTSGGGIIVIKPALSVTIR
                     HEQYPGFKIELWGMEVVNGTAKLVGNHENLNGKHIKDKETGRRSVILPDGAK"
BASE COUNT          854 a          832 c          725 g         1047 t
ORIGIN      
        1 tacgatattc gcttttggtc aggtaaataa agcggccgcc tggctacttg tgccatatat
       61 tgcctgggta tcatttgcag gtatactgaa ttatacaatc tggagattga attaaggcgg
      121 gcagaaaaca gccgttacat actatgaaac tctatataaa ccttctaaac taaccaccat
      181 ccggcaataa cccaaagtac tgtatcagaa ctggtactac gaattgagct ttacctaaac
      241 cttctaaact atcatctgcc gggcattaac agaaatgtcc tttttagagc tggcactact
      301 aaatgaaaaa aatctaaacc tttctagaat ttattcttcc acatcatgct ctccaccggt
      361 ttgtccggct gattgctgta ttttgaattc tttttctggt tgtatttgat ttcaatttca
      421 ccgtctaccg ggaaataaat caattggccg attggcattc ctttatatac tttgaccggt
      481 tgcttaacag atatttcgag ggtccagtta ccgcagaacc ccacatctcc ttttccggcg
      541 gtggcatgga tatcgatgcc cagtcttccg gtggaggatt tgccttccag gaaaggcacg
      601 tgggcatggg tttccgtgta ttcctcggtt accccaagat aaaagatatg ggggtataaa
      661 acgattcctt catcgggaat ctcaaaatag tcgatctcat tgtgcttttt cgcatcgatc
      721 atatgctctt tgtacgtagc cagggtcttg cccaggtgta catcgtagga attgctgcca
      781 agacagtcac gctgataggg gaccacttta atggtgcctt tctccatttc ctccagaatg
      841 cgggtatcag aaagtatcat ttatcttcgt ttttaataag ggcagcaaag taaacaggaa
      901 taagaaagga atgccggttt tttttcaaca acaaatcaac gaatcgaccc gtttgggggt
      961 ctggaagata gaagaaacgg aagctttctt ccggggaaat gtgcccatac accgggacgt
     1021 gacccatccg aaaaagcggc tgcagcacct ggccggacgt tttctgctgc aatatttgtt
     1081 tcctgatttc ccttacgaac agatccggat cgctgacaca cgtaagcctt acctgccgga
     1141 tgaacgctat catttttcca tctctcattg cggggattat gcggcggcca ttgtcagtcg
     1201 tacccatcgg gtgggcgtgg atgtggaaga aacggaagac cggatcctcc ggatcaaaga
     1261 caaatttatg tccgcgaaag aacaatccct ttttataccg gatcccttta cacccgatta
     1321 ccggttactc accttactct ggtccgccaa ggaatcagtc tacaaatggt acggagcagg
     1381 ggaggtggat ttcaggagac atattcttct gagtgaattg gatgtgacgg ataataagct
     1441 acgatgtgat ttcgggctta caggagatcg gttgaaagtg gagttcaggt tttttgacgg
     1501 gttggtactg agttgggtgg ttgatagatt tacaagttaa caagttgata ggttgactag
     1561 gacctcctaa ggaattattg ttaaatttta cggccacttc atccttttca acttctcaac
     1621 cagtctttaa tccaaaacga gatcatcatt cccatctccc aacaaattca aatccacatc
     1681 tgcgccggaa ataccttcaa tagaaccccg gataccagtg gcatttatca tcactttttc
     1741 taactgtttt tcacgggcct tccagagttt ttccatcgca tccctttctt tctggatcga
     1801 gagtttcata ctcataaatc cttcccggat agcagaccac tgatcggcaa attcgcggct
     1861 ggtaaggtat ttatataaaa gatgcatttt gtctcccttg ttttcctggc tgcgggcggc
     1921 attggccaca ttgatgatgc cataacggat catttgcacc aatggcttta cttccgcaaa
     1981 actgcagatc cacacgccat ctttttctcc aaaacggtcc atgtcttttg gtaaagcctg
     2041 ggaaacaatt accgccacat ccgctccctg gctgcgcata tcggctttta atttctcgat
     2101 ccagtcattt ccaaaatcct tggtgcgctt gctctcaaaa atgatcttgc cacattcctg
     2161 gccaaagtta ttgcgaacgg tttgaatgca gtcagccccg cgtacccctt tccccacttc
     2221 actgaccagg tcgaagggga aagctgtttt tagtaattcc tccagcaaca gttcctgaac
     2281 ctctccctgg gtctgcatac ttccctgttc agctttacgc cgcatttcat cggccagttt
     2341 cttctggtca tccagctgct tctgcagttc tttcagctgt aactggaatt cggtttcttt
     2401 tgcactcatc cgctgttctt ccagtttgcg tatctccacc gacaattttt ccctttcttt
     2461 ctgaagttgc tgctggaccg atagctccag ttccgcttcc ttatttttca gttcctgctc
     2521 tttttttaag aattccagtt gctgtttccg ggcctgctgc agtttttctt catagtcttt
     2581 gttattctgc tccgccattt tcagctgggt ttcaaaatca gctgcgatgc tcttacggag
     2641 atttccctca aggctttcct gcaatctttt cttttcctcg tccatttttt gctggaattc
     2701 ctcattcttc tttttctgcc agtccgctat tttagcccgc agttcctgtt ccacctgatc
     2761 ccggatggca tcattgggtt caaactggtg gccgcaattc ggacacttga tttccgtcga
     2821 catgctttgg tattttccca aaactacaaa taccagcggg aatagacaat tcaaccacta
     2881 atcagcgctc cgatcattca gtattgtcca aaaaaaatga aaatacctga tgactatttg
     2941 agaaagatcc gatgtattga tatccggaat gcccgggtca cctctaaata aagaatatgt
     3001 caaaaagcag ttttttgatt catccgcagc aactattttt ttcgaccgaa tcccagacca
     3061 gtaattttta tccggcttgc aggctgcccc gttttttggg gatatgttta ttttctatac
     3121 tgatattcag ttcttgtaag aaaggggata cgcccgcggc agaagaactg aaactgggta
     3181 cctgtgccgc ggtcacacct aaagccggta tgatacagtc tggtgttgcc gattcatttg
     3241 tgtaccggac cagtggaggg ggaatcattg taatcaagcc tgcactttcc gtaaccatcc
     3301 ggcatgaaca atatccggga tttaaaattg aactttgggg tatggaagta gtgaatggta
     3361 ccgcaaaact ggtgggcaat catgagaatc tgaatggtaa acacattaag gataaagaga
     3421 ccggccgccg ctcagttatt ttacccgatg gcgccaaa
//
LOCUS       DAOPMW010000371         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_32714_length_3454_cov_3.498088, whole genome shotgun sequence.
ACCESSION   DAOPMW010000371 DAOPMW010000000
VERSION     DAOPMW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32714_length_3454_cov_3.498088"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3454)
                     /locus_tag="PLU11_13725"
     CDS             complement(<1..>3454)
                     /locus_tag="PLU11_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPN60239.1"
                     /translation="KRVFRSSSVLLFFFFLFVSASAQKNNWPNTLLWKISGNGLSKPS
                     YLFGTMHLQDKRIFNLGDSFYHHFERAEGFAIEVDFREYMDSLLTKGFQMAEDKNLKD
                     EEDEYSDAVIDTSVIQKPPPPSADMPAVKSDAEKKMSRNLRKEFRKMRNEQIKSLLLH
                     GRMPTILDAYLYGMAMKQGKWLGAVEELNDQLSIRDELGKDIDEKEEMEQPERILLTS
                     LGNMIKVYLAQDLNQIEEIALNQQSAKTKTIVFNNRNLKMARSMDSLSHLRSMFYAVG
                     AAHLPGDSGVILLLRQKGYTLTPVISTSTMAAEKYAAGLPSLAWYEVGQTDGLYKVDM
                     PGIPSEYNLYGELVKMKVYVDVTTMNFFMAGHTIAQFGDDELESAFKNMGKSMGGKII
                     NIKKFSRDEAKGVEGMVNNGSTSFRLQMLKKGNSAFFLMVGSSGSYKISNADADKFFY
                     SFKAGETARSSTPSGWKEFSLPDKGVTVKMPAVPKRNKSFERQAEGSGWNFQVYDCTD
                     PAAGLYYLFQVRDVSAGNFLEGDSLYFEQFKENLLKEDVVKLRDETAVIGGQPVLRFD
                     ATSKSESLFYKTLNLIRGNRVYTLMVLGHVSLKDEEGAEHFFQSLKLEELKKTVFTLQ
                     TADDLSFSSRVPDKFVRAIDEEKEDSTRIHYTSYDPNEVTSYEVVKDRLPPLFWVKSD
                     TAFYRKRASLAVGYDDSLLHYNIVQNGKIRGVEQLVQLSGSSMVKRMRYLLHDDTLYT
                     LVSYIPAAIASDKYHTEFFEQFRIHNDHSVSSILKSKAAALLESLASKDSLQFTRASE
                     VIDLVSFEKSDLPLLHKALLGTYYDDTLSWNNARNKLVRAIEPVADSSTVDFIKTNYA
                     LLADRGGDQLAMLNVLVNYKDRYAYEALKDLFVRKPPVNPGDRYSVSYRMTDSLELTK
                     ILFPEILDLLKNEHYWDNITDYTVRLLDSGVVTREILLPQTKVLFHIADTLLTGGTLD
                     SEEVWSWKYSSLLELFGYINSGESNQYLQRFLKGKDLYLKSLAALRLVKNGQTVDPAE
                     LTKLAADNEYRLDLYNDLKELGKTSLFPAKYLTQRYFAEAELYTYASDDYSPSSMEYI
                     GERLAELNGQKMRFYLFKIGFEDEEGNDTESYLGMAGPYEIDQKNLVTTNDATGYQYD
                     EAYNK"
BASE COUNT          870 a          894 c          695 g          995 t
ORIGIN      
        1 tcttattgta cgcttcatca tattgatacc cggtggcatc attggtggtc accaggtttt
       61 tttgatcaat ctcataagga cccgccatac caagatagga ttccgtatca tttccttctt
      121 catcctcaaa accgatttta aaaagataaa aacgcatttt ctgtccgttc agttcagcca
      181 gcctttcccc tatatattcc atggaggaag gagagtaatc atcagaagca taggtatata
      241 attccgcttc tgcaaaataa cgctgcgtca ggtacttcgc cgggaacagg gaagtcttcc
      301 ccagttcttt caggtcgttg tacaaatcca gccggtattc gttatcggct gccaacttcg
      361 tcagttctgc cggatcaacc gtttgaccat ttttcaccag gcgtaatgcc gccaggcttt
      421 tgagataaag gtccttccct tttaaaaacc gttgtaaata ctgattcgat tcaccgctat
      481 tgatataccc gaaaagttcc aacagactgc tgtatttcca gctccaaacc tcctcgctgt
      541 ccagtgtacc accagtaagc aatgtatcgg caatatgaaa gagcaccttt gtttgaggca
      601 ggaggatttc ccgggtgacc actccgctat ccagtaacct gacggtataa tccgtgatgt
      661 tatcccagta atgctcattc tttaataaat ccaatatttc gggaaacagt attttcgtca
      721 gttccagcga gtccgtcatc cggtaactga ccgaataacg atcacccgga ttcacaggcg
      781 gtttacgcac aaataagtcc ttcagggctt catacgcgta ccggtccttg taattcacca
      841 gcacattcag catggccagc tgatctccac cccggtctgc cagtagcgca tagtttgtct
      901 taataaaatc caccgtactg ctatccgcta ccggttcgat ggctctgacc agtttgttgc
      961 gggcattatt ccatgaaagg gtgtcgtcat aataagtgcc gagtaatgct ttatgcagca
     1021 gtggcaaatc gcttttttca aaactgacca gatcaatcac ttcactggcc cttgtaaact
     1081 gaagactatc ttttgatgcc agtgactcca gtaaagcagc cgctttactt ttcaggatgg
     1141 aagaaacgga atgatcattg tgaatccgga actgttcaaa gaattcagta tggtatttgt
     1201 cggaagcaat ggccgcgggt atatagctga ctaatgtata tagagtgtcg tcatgcaaca
     1261 gataacgcat tcgctttacc atactgcttc cggaaagttg taccagttgt tccacgcccc
     1321 tgatcttccc attctgtaca atattgtagt gtaataaaga gtcatcataa cccacggcta
     1381 aagaagctct tttccggtaa aaagcagtat cagatttaac ccagaacaac ggaggcagcc
     1441 tgtctttcac cacttcatag gaagtcactt cgttcggatc ataactggta taatgaatac
     1501 gggtactgtc ttctttttct tcatctatcg ccctgacaaa tttatccggc accctgctgc
     1561 tgaagctgag atcatcggcc gtttgcaggg tgaataccgt tttcttcagt tcctccagct
     1621 tcagtgactg gaaaaaatgt tccgccccct cctcatcttt cagggacaca tgacccagta
     1681 ccatcagtgt atagacccgg ttgccccgga tcaggttcag cgttttataa aacagggatt
     1741 ccgatttgga cgttgcatca aaacgcaaca ctggttgccc ccctattaca gcggtctcat
     1801 cccggagttt taccacatcc tctttcaata agttttcctt gaattgttca aagtagagtg
     1861 aatccccttc caggaaatta ccggccgaaa catccctcac ctggaaaaga tagtagagtc
     1921 ccgcagcagg atctgtacaa tcataaacct gaaaattcca acctgatcct tctgcctgcc
     1981 tctcaaaact tttgtttctt tttggcacgg cgggcatttt tactgtaacc cctttatccg
     2041 gcagactgaa ctctttccag ccggatggag tgctgctgcg ggctgtttca cctgctttaa
     2101 atgaatagaa aaatttatcc gcatcggcat tgctgatttt gtaagaaccc gaactgccca
     2161 ccatcaggaa aaaagcactg ttcccttttt tcagcatctg cagccggaaa ctggtgctcc
     2221 cattatttac catcccttcc actccctttg cctcatcccg gctgaacttt ttaatgttta
     2281 tgattttccc tcccatggat ttgcccatat tcttaaatgc actttcaagt tcgtcatcgc
     2341 caaattgagc aattgtatga ccggccataa agaaattcat ggtagtgaca tccacataga
     2401 ctttcatctt taccaattcc ccgtaaagat tgtattccga cgggatgccc ggcatatcca
     2461 ctttgtacag accatctgtc tgccccactt cataccacgc aagggaaggc agacccgccg
     2521 catacttttc cgccgccatg gtgctggtag atatgaccgg ggtaagggta tagccttttt
     2581 gtctcaataa aagaataaca cctgaatcac cgggcaggtg agcggcccct actgcataga
     2641 acatactgcg gagatgactt aaactgtcca tactcctggc cattttgaga ttgcggttat
     2701 tgaagacaat cgtcttggtc tttgcacttt gctggttcag ggcaatttcc tcaatctggt
     2761 tcagatcctg ggccaggtac actttaatca tattgcccaa tgaggtgagc aggatccgtt
     2821 cgggttgttc catttcttct ttctcatcaa tatccttacc cagttcatca cggatactca
     2881 gctggtcgtt taattcttcc actgcaccca gccatttgcc ctgcttcata gccattccgt
     2941 ataagtaagc atccaggatg gtgggcattc gcccatgcag caacagactc tttatttgtt
     3001 cgttccgcat tttccggaat tccttccgca ggttacggga catttttttc tctgcatccg
     3061 atttcacagc cggcatatca gccgatggag gtggtggctt ctgtattacc gacgtatcaa
     3121 ttacggcgtc gctgtactca tcctcctcgt ccttcagatt tttgtcttcc gccatctgaa
     3181 aacccttggt caggagtgaa tccatatatt ccctgaaatc cacttcaatc gcaaaacctt
     3241 ccgcccgttc aaaatgatgg taaaatgaat cccccaggtt aaagatccgt ttgtcctgca
     3301 ggtgcatggt cccgaacaga taagagggct tgctgagtcc gttcccgctg attttccata
     3361 aaagggtatt aggccagttg tttttctgcg cagaggcgga aacaaaaagg aaaaaaaaaa
     3421 acagcagtac ggaactgctc cggaacactc tttt
//
LOCUS       DAOPMW010000372         3429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33029_length_3429_cov_7.891523, whole genome shotgun sequence.
ACCESSION   DAOPMW010000372 DAOPMW010000000
VERSION     DAOPMW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33029_length_3429_cov_7.891523"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLU11_13730"
     CDS             complement(<1..182)
                     /locus_tag="PLU11_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN60240.1"
                     /translation="MIIDSHQHFWLYEPEKHSWIDEEMKSIRRDFLPQELAAVFKENG
                     VDGSVAVQADQKEIETA"
     gene            complement(371..1225)
                     /locus_tag="PLU11_13735"
     CDS             complement(371..1225)
                     /locus_tag="PLU11_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPN60241.1"
                     /translation="MKLIRFGAPGEEKPGIHINGTNYDLSAFYRDYDELFFADNGLAD
                     LAALLKMSAGKLPVVPEGTRLGPPVARPSKIVCIGLNYSKHAKETNAPIPKEPIIFFK
                     STTSYNGPNDPITIPKNSVKTDWEVELAFIMGKKASYVEVSEAMDYVAGYALHNDVSE
                     REFQLERGGTWDKGKGCDTFAPIGPWLVTKDEIPDPHNLRLWLTLNGKMMQDSNTNDL
                     IFNIPFLVSYISQFMTLLPGDIISTGTPAGVGLGFNPNIFLKEGDVVELGIDGLGTSR
                     QVCYDYKK"
     gene            complement(1234..1995)
                     /locus_tag="PLU11_13740"
     CDS             complement(1234..1995)
                     /locus_tag="PLU11_13740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN60242.1"
                     /translation="MFSLSNKTALVTGGGSGIGRAISTLFAKQGASVYILDLDEKGAT
                     QTVEEIKAAGGQAGFIICNVSDQQAVKAAVAQLPAIHILVNNAGVAHVGNAENTAVED
                     FTRIFEINVKGIYNCLHEVIPVMKQTGGVILNMASIASSVGITDRFAYSMSKGAVVGM
                     TLSTARDFMAAGIRCNCISPARVHTPFVDGFISKNYPGKETEMFEKLSKSQPIGRMGH
                     VDEIAALALYLCSDEASFITGNDYPIDGGFIKLNN"
     gene            complement(2009..2347)
                     /locus_tag="PLU11_13745"
     CDS             complement(2009..2347)
                     /locus_tag="PLU11_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPN60243.1"
                     /translation="MKRYCLALDLVDDPTLIAEYEKWHKPGAQLPGIPESIKAAGILQ
                     MEIYRTGNRLFMIMETTDNFDPARKAAADASDPAVQEWEQLMWKFQQPLPWAAPGEKW
                     VMMQQIFQLT"
     gene            complement(2344..>3429)
                     /gene="fucP"
                     /locus_tag="PLU11_13750"
     CDS             complement(2344..>3429)
                     /gene="fucP"
                     /locus_tag="PLU11_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HPN60244.1"
                     /translation="KFGYKAGVILGLVLFAIGSFLFIPAANTQQYAFFLGALFVIASG
                     LTFLETAANPYADLLGDPETSTQRLNLSQSFNGLAATMAPIIGVNLILTKDYSNVELA
                     GMTEEARKLALAAEASTVKTPYLILGLCILLVAIIFWFTHLPEVKQTEKTGKEKSKSI
                     LHAFRHAHLSWAVVAQFFYVGAQVCVFSFFLLYAPKAAGVSDRVAGIFLGWGCGLAFM
                     FGRFFGTYLMVEMVKPARLLSIYALINIVLCAVAMFASGIPALIAVIGIAFFMSIMFP
                     TIFSLGIKDLGTDTEYGSSLIIMSIVGGAILPPVFGWIADSTGNIQLAYIVPLLCFAV
                     ILLFGLKGYKVKNLNEAQKKSTINIIR"
BASE COUNT          883 a          815 c          811 g          920 t
ORIGIN      
        1 gcggtttcga tttctttctg atcggcctgt acggcaaccg aaccatctac tccgttctcc
       61 ttaaaaacag cagccagttc ttgcggaagg aaatcccggc ggatcgattt catttcttca
      121 tcgatccagc tgtgtttttc gggttcgtag agccagaaat gctggtggct gtcgataatc
      181 atgagggttt gttgtttgtt gtttgttgtt tgttgtttgt tgtttgttgt ttgttgtttg
      241 ttgtttattg tttagttgtt tagttgttta gttcactttc tactcaccct tcggcaagct
      301 cagggtgaca accggtgtcc ttgtcatggt gagcttgccg aaccatgaac ttgcagaagc
      361 ataaaccaaa tcacttctta taatcataac aaacttgccg gctcgttccc aatccatcaa
      421 tccccagttc cactacatcg ccttctttca ggaagatatt gggattgaag ccgagtccga
      481 ctcctgcggg ggtgccggtg gatatgatat cgccggggag cagggtcatg aactggctga
      541 tataggaaac gaggaaggga atattaaaga tcaggtcatt ggtattgctg tcctgcatca
      601 tcttgccgtt gagggttaac cagagccgga gattatgcgg atcaggaatt tcgtcttttg
      661 tgacgagcca gggaccgatg ggggcgaatg tatcacaacc ttttcctttg tcccaggtac
      721 cgccgcgttc cagctgaaat tcacgttcac tcacatcatt gtgcagcgca tagccggcca
      781 catagtccat ggcttcactc acttccacat aggatgcttt tttccccatg ataaatgcca
      841 gctctacttc ccaatccgtc ttcacggaat tcttaggaat cgtaattgga tcattggggc
      901 cgttataaga agtggttgat ttgaaaaaga tgatcggctc tttggggatc ggggcattgg
      961 tttccttcgc gtgtttggaa taattcaatc cgatgcatac gatcttggaa ggtcgggcca
     1021 ccgggggacc taagcgggtt ccttcgggca ctactggcag tttgcctgct gacattttca
     1081 ggagcgctgc aagatcagcg agtccgttat cggcgaaaaa gagttcatca taatcccggt
     1141 agaaggcact caggtcataa ttggtcccgt taatatgtat gccgggcttt tcttccccag
     1201 gtgcaccaaa acgaatcagt ttcatattgt gtattaatta ttcagtttga taaatccgcc
     1261 atcgatcgga taatcgttgc cggtgataaa tgaagcttcg tcactgcaga gatagagcgc
     1321 cagggcggct atttcatcca catgacccat acggccgatg ggctggctct ttgataactt
     1381 ttcaaacatt tctgtttcct tacccggata gttcttggaa ataaatccat ctacaaaagg
     1441 cgtatggaca cgggccggag aaatacaatt gcaacggata cccgcagcca tgaaatcacg
     1501 ggccgtggaa agagtcatac caaccaccgc acctttactc atgctatagg caaaccgatc
     1561 ggtaataccc acggaggagg ctatggaagc catatttaaa atgacgccac ctgtttgttt
     1621 cattacggga atcacttcat gcagacaatt gtaaatccct tttacattga tttcaaaaat
     1681 gcgggtgaaa tcttccaccg cagtattttc cgcattcccc acatgggcca ccccggcatt
     1741 attcaccaaa atatgaatag cgggtaattg agcaaccgct gcttttacag cctgctgatc
     1801 ggaaacatta caaatgataa atccggcctg accgccggcc gctttaattt cttccacggt
     1861 ttgtgtggca cctttttcat ccaggtccag tatataaacc gaagcaccct gtttggcgaa
     1921 aagcgtggag atcgcccggc cgataccact acctccaccg gtcacgaggg ctgttttatt
     1981 ggatagggaa aacatgattg aaattatatc aggttaattg aaagatctgt tgcatcatta
     2041 cccatttttc tccaggagcg gcccagggta agggttgctg gaatttccac atcagttgct
     2101 cccattcctg tacggctgga tcagatgcat ccgctgctgc tttacgggcc ggatcaaaat
     2161 tgtcggtggt ttccataatc ataaatagcc ggttgcccgt gcggtaaatt tccatctgca
     2221 ggataccggc ggctttgatg ctttcgggta tcccgggtaa ctgggctccg ggcttatgcc
     2281 atttctcata ttcggcgatc aatgtgggat catccacgag atcaagggcc agacaataac
     2341 gtttcatcgg attatgttta tggttgactt tttctgggct tcattcaggt tcttcacttt
     2401 gtaccctttc agtccgaata aaagtattac tgcaaaacat aataaaggta caatataggc
     2461 caactgaatg ttccctgtac tgtctgcaat ccaaccgaaa accggaggaa gaatggcgcc
     2521 acccacaatg gacatgatga tgagactgct gccatactct gtatccgtac ccaggtcttt
     2581 aatacccagg gagaaaatcg tcgggaacat gatggacata aaaaaagcga taccgatgac
     2641 ggctatcaga gccggtattc cggaagcaaa catcgccacg gcacataaaa caatattgat
     2701 aagtgcataa atggataaga ggcgggcggg ttttaccatt tccaccatca ggtaagtacc
     2761 aaagaagcgg ccaaacataa aggcgagccc gcaaccccat ccgagaaaaa ttccggccac
     2821 tctatcactt acccctgcgg cttttggcgc atagaggagg aagaaactga atacacaaac
     2881 ctgggcaccg acataaaaaa actgtgccac caccgcccag gataaatgag cgtgtcgaaa
     2941 cgcatgcagg atggatttac ttttctcttt acctgttttt tcggtctgtt ttacttccgg
     3001 caaatgggtg aaccaaaaga taatggcgac taataaaata caaagtccca ggatcaggta
     3061 gggcgtcttt actgttgagg cttcagcagc cagggccagt ttacgggctt cttccgtcat
     3121 accggccagt tcgacgtttg aataatcctt tgtaagaatc aggttcaccc cgattatggg
     3181 ggccatggtg gcggcgagtc cgttaaagga ttgcgacagg ttcaaccgtt gtgtggatgt
     3241 ttccggatca cccagcagat cggcatacgg atttgccgcc gtttccaaga atgtgagtcc
     3301 gctcgcaatc acaaataaag cacccagaaa aaatgcatat tgctgggtat tggcagccgg
     3361 tataaaaaga aaggacccaa tagcaaagag gaccagtccc aggatcaccc ccgctttata
     3421 accgaattt
//
LOCUS       DAOPMW010000373         3408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33288_length_3408_cov_5.059946, whole genome shotgun sequence.
ACCESSION   DAOPMW010000373 DAOPMW010000000
VERSION     DAOPMW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33288_length_3408_cov_5.059946"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..2088
                     /gene="recN"
                     /locus_tag="PLU11_13755"
     CDS             415..2088
                     /gene="recN"
                     /locus_tag="PLU11_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPN60245.1"
                     /translation="MLQRLSISNYAIIDELEIDFSGKLNVITGETGAGKSIIMGALGL
                     ILGERADTAALVKKEKKCVVEGLFQAGQKKAVAAFLKANELDNEDQLVLRREISTNGK
                     SRAFINDTPVNLSQLQQLSALLVDLHQQFDTLELGESGFQREVIDALAGQAALLDEYL
                     QAFRKWQVVKKEAEELKLQKQLFDKEADYNRFQFTELEEAGFKENELEDTDAELKLLN
                     NAEGIKAALNKTFFELRESESPMVQQLKIMINQLNPYTGYHPDLPTLLQRLQSAQIEL
                     QDIAGEVDRISDHVNYDPEKVERLNERLSLGYKLQKKHGVNTTAELLSIQAGLQDKLQ
                     AVLNIDQSIAQKEKEKQQLYDSASMLAQKISANRKKQVKPFEDKVNKLLHQVGMPNAR
                     IKTMINPVELMETGADNVEFLFDANVPAGDQAKGQYQPLRKVASGGELSRLMLCIKSL
                     VAQSVDLPTMIFDEIDTGISGEAARQVGIIMKELAAKRQVICITHQPQIAGKADAHVF
                     VFTEIVKDQVKTNIRLLSTEERITSIARMLSGEKPTAAAMENAREMLMN"
     gene            2269..>3408
                     /locus_tag="PLU11_13760"
     CDS             2269..>3408
                     /locus_tag="PLU11_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60246.1"
                     /translation="MKAHQPFLLGLFLFIQLISNAQISWYQSQDGQLQPSGTYAAGIL
                     SLTRQSFVAAYMWRLDNENYTWKISKSHTNGTEQRTFFITAPYGIVEMKTGYRNSLYL
                     LVRSFPFAQDPQYTFYKLDSNLVVKAQRTISLAGSFTIFNLNVFELDEAGNLYLAGDG
                     QYPDGPGFSPASFIMKTDKNLVTRWTRMDSVQTSYSRLHIERNGTIRLIEDFYTFFPD
                     IKVQKISAAGQLLQTRVYQTDPGRQSLTTILDRDDNLLIAGTMDRAAGQSLYLHKIAR
                     HSGQVVYRKTFFNALAANLDDVKLDEDGQLYALMTQYYNNGRQSRLARIQPRQGSLSW
                     SRTFPFAQDSCMLRTIVLSDEDRIYVIGDRKSGPYYTKGFAVQVKR"
BASE COUNT          969 a          757 c          816 g          866 t
ORIGIN      
        1 gccagcacaa aaagatcatg atctgcgaaa actgcggtcg tatcctggtg gatgaagagc
       61 tgagcaataa tgtagagatc aagtagtttt cttagaaaat attgttagta cgagaaggtt
      121 ctgtttttac agaacctttt ttagttagta gatagtagtt agaagttagt agtcaacagc
      181 ccctcctgag aaaagttgtc agatgtcaga tgtcggatgt caggaaacag ccccttctaa
      241 gtgagattta tctgatccgt gggtatctgt gcaatccgtg gcctgccttt ctgtattctt
      301 tcgtgccctt ttgtgtattt cgtggccacc ccgctgccgc aggcagcatt tgcgtagcaa
      361 atccttttcc accgtggccc aacttgtagc gaaaaaactt aatttgcccc ttcaatgctc
      421 caacggctct ccatatcgaa ctacgccatc atcgacgaac tggaaattga tttctccggc
      481 aaactcaacg tgatcaccgg tgaaaccggt gccggtaaat ccattatcat gggcgccctc
      541 ggattgattc ttggcgaacg ggctgatacc gccgctttag tgaagaaaga aaagaaatgt
      601 gtggtggaag gcttattcca ggccggacag aaaaaagccg tggccgcttt tctcaaagcc
      661 aatgaacttg acaatgaaga ccagctggtt cttcgtcgtg aaatcagcac caatggtaaa
      721 tcacgcgctt ttatcaatga taccccggtc aatctttccc aacttcaaca attaagcgcc
      781 ttactcgtgg acctgcacca gcagttcgat acgcttgaac tgggcgaatc cggctttcaa
      841 cgggaagtga ttgatgcgct ggccggacaa gccgctttgc tggacgagta tctgcaggct
      901 ttccggaaat ggcaggtcgt aaagaaagaa gcggaagaat taaaattaca gaaacagtta
      961 ttcgataagg aagccgatta taaccgtttc cagtttaccg aactggaaga agccggcttt
     1021 aaagaaaacg aactggagga tacggatgcc gaactgaaac tcctgaataa tgcagaaggg
     1081 atcaaagcgg ctttgaataa aacattcttt gagttgcggg agagtgaaag tcccatggta
     1141 cagcaactaa agatcatgat caatcaactg aatccttata ccggatatca tcctgatctg
     1201 cccacccttt tacaacgcct gcaatcagcg cagatcgaat tacaggatat tgccggggaa
     1261 gtggatcgca tcagtgatca cgtgaattat gatcctgaaa aagtggagcg cttgaatgaa
     1321 cggctttccc ttgggtataa actccagaaa aaacatggag tgaataccac cgctgaactt
     1381 ttatccatac aggccgggct gcaggataaa ctacaggcgg tgttgaatat tgaccagtcg
     1441 atagcgcaga aggaaaaaga aaagcagcaa ttgtatgata gtgcatccat gctggctcaa
     1501 aagatatcgg ccaatcgtaa aaagcaggtg aagccttttg aagacaaggt caataaactc
     1561 ttgcatcagg taggtatgcc caatgcccgg ataaaaacaa tgatcaatcc ggtagagctg
     1621 atggaaaccg gggctgataa tgtggagttt ttatttgatg ccaatgttcc cgccggtgat
     1681 caggccaaag gtcagtatca accccttcgc aaagtggcca gtggaggaga actgagccgg
     1741 ctgatgctat gcataaaatc gctcgtggcg caatccgtgg atctgcctac gatgatcttt
     1801 gatgaaattg atacgggtat ttccggtgaa gcggccaggc aggtgggcat cattatgaaa
     1861 gaacttgccg ccaaacgaca ggttatctgc atcacacatc agccacagat tgccggaaag
     1921 gccgatgcgc atgtcttcgt gtttacggaa attgtgaaag accaggtgaa aaccaatatc
     1981 cgcctgctta gtaccgaaga gcggattacc agtatagcac ggatgctcag tggcgaaaaa
     2041 cctacagcag ccgctatgga gaatgcacgt gaaatgctga tgaattaagt acaatactgt
     2101 cttttttagc ctgttgtttt ttctcgaaat aacccgtcgg ttctcctgcc ttctacgcag
     2161 tatcattcct ttttttgaaa aacattcccg ccgtaaatgc aggtcagtag cctgatgcat
     2221 gattattgta caggtagtat tgatctttaa gcactaaaat ccgtatgtat gaaagcacat
     2281 caacccttcc ttcttggcct ttttttattt attcagctaa tcagtaatgc tcagatttcc
     2341 tggtatcagt cgcaggacgg acaactccag ccgagtggta cttatgccgc cggaatttta
     2401 tccctgaccc gacaatcttt tgtggccgct tatatgtggc ggctcgacaa tgaaaattat
     2461 acctggaaaa tttccaaatc acatacgaat gggaccgagc aacgtacttt ttttataacg
     2521 gcaccttatg gtattgtgga aatgaaaacc ggttaccgga acagccttta tctgcttgta
     2581 cgcagttttc ctttcgccca ggatccacag tacacttttt ataaactgga cagtaatctg
     2641 gtggtaaagg cacaacgaac aattagttta gcggggagct ttaccatttt taacctgaat
     2701 gtttttgaat tggatgaggc cggaaatctt tacctcgccg gagacggaca atatcctgat
     2761 ggacccggat tcagtccggc ttcttttatt atgaaaacag ataagaacct ggtgacccgc
     2821 tggacccgca tggattcggt acagacttcc tattcccgct tgcatattga acggaatgga
     2881 acgatcagac tgatcgagga tttttatacg ttcttcccgg atattaaagt gcaaaagatc
     2941 agcgcggccg gtcagttgtt gcaaaccagg gtataccaga cagatcctgg tcgtcagagt
     3001 ctgaccacca tactggaccg ggatgacaac ctgctgattg caggtacaat ggatagagca
     3061 gcaggtcagt ctttatacct gcataaaata gccaggcata gcgggcaggt tgtataccgt
     3121 aagacatttt tcaatgcgct ggcagcaaac ctggatgatg tgaaactgga tgaagacggg
     3181 caattgtatg ccttgatgac gcaatactat aataacggaa gacagagcag gctggcccgg
     3241 atacaacccc ggcaaggatc actctcctgg agccggactt ttccgtttgc acaggacagt
     3301 tgtatgctcc gcactatcgt actatcggac gaggaccgta tttatgtcat aggggaccgg
     3361 aaaagcgggc cgtattatac aaaaggattt gcagtacagg ttaaaaga
//
LOCUS       DAOPMW010000374         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33369_length_3403_cov_9.215352, whole genome shotgun sequence.
ACCESSION   DAOPMW010000374 DAOPMW010000000
VERSION     DAOPMW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33369_length_3403_cov_9.215352"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..667)
                     /locus_tag="PLU11_13765"
     CDS             complement(191..667)
                     /locus_tag="PLU11_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60247.1"
                     /translation="MNLLFRAKYKYKLNESPAQVKAELETLFSTPWHKTAPRLSGYFS
                     NDFSFKIRSVLSTAVAFFGIFQSFAVLQGRLQAEEERTVIRLTARPGTISLLLFYALV
                     VVTILAAVKAQAALTPETIVVAAALTGLLISYYFILRFSRNSLRRYFQRQMGIKSK"
     gene            complement(790..1311)
                     /locus_tag="PLU11_13770"
     CDS             complement(790..1311)
                     /locus_tag="PLU11_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60248.1"
                     /translation="MLLLVLTAGITGSSYACTCIGKDKQTTESEFSSSKLVVKGKIIA
                     STDYLYYDTLITLFTGIPFDRATSGYMIRQYKIYTLVVESRFKSDKGLADTLRIVTGY
                     GNGDCGYEFETGKDYIVYANAWTEKKLIIKQRKKKIKKKIREEPVTNWFYTDICRLTQ
                     ESNQLELDKIKNL"
     gene            complement(1383..1898)
                     /locus_tag="PLU11_13775"
     CDS             complement(1383..1898)
                     /locus_tag="PLU11_13775"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60249.1"
                     /translation="MISIVKAQVKDATLLAGLATQTFIESHGHSAAAADIDAYVAENY
                     TPDILQAELEDPANIYHILYYHKLPVGFSKILLNTSYEGSPGNAITKLERIYLLKSYY
                     GTNLGPALMDFLMHLMKKEKQTGVWLYVWKENIRAIRFYEKKGFKITGSHDFPISPSH
                     SNPNHRMLLEF"
     gene            complement(1931..>3403)
                     /locus_tag="PLU11_13780"
     CDS             complement(1931..>3403)
                     /locus_tag="PLU11_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPN60250.1"
                     /translation="TFQGFRYIKVEGFPGELTADNLTAVALYSDMPPTGTFTSSNKLI
                     NQLQHNIQWGQKGNFLDVPTDCPQRDERLGWTGDAQVFSRTAAFNFGVNNFFAKWMKD
                     VAADQLENGSVPFVIPNVLGAGSAGSTGWADAASIIPWNMYLAYGDKKILEDQYPSMK
                     AWQGFMEKNSKDDLWATGFHFGDWLFYRPFDDNDGRAAVTDKYLIAQCFFAHSTDLMR
                     RTATVLGNTADADHYRNRLEKIKAAFRKEYMTANGKLVSGTQTAYTLALQFDMLPEEL
                     RQTAAARLVENIKSYGYHLTTGFLGTPYLCHVLSRFGYADIAYRLLLQETYPSWLYPV
                     KMGATTIWERWDGIKPDSTFQVPSMNSFNHYAYGAIGDWMYRVAAGLDTREEAPGYQQ
                     LRIEPHTGGSFTQLEARLKTNYGEAVSGWKISNQLLILEISIPVNSSALIYLPSSDLS
                     AITENGVPLSGHKDLTLQGTENGKIVVKAGSGQYRFEIKK"
BASE COUNT          903 a          779 c          775 g          946 t
ORIGIN      
        1 ttccgggttc acacacaatt tttgtagtgg cacaaattag gggaaagatg gggtgcagac
       61 ttcttttttt tattgggaaa acacgatttt ttccatgatt tccggcaatc catggattta
      121 gccttaaact gtggatttca tgtataaaaa agaagggaag ggaatgctat tctttcagga
      181 atactgcttt ttatttcgat ttaatcccca tctgccgttg aaagtatcgt ctcagactgt
      241 ttctcgaaaa gcgcaggata aaataataac tgatcagcag ccctgtaagc gcggcggcca
      301 cgacaatagt ttcgggtgta agggcggcct gggctttgac agcagcgagt atcgttacaa
      361 ccactaaggc gtaaaaaagg agtaaggaga tagtgccggg acgggcagtt aaccggataa
      421 cggtccgttc ctcttcggcc tgcagacggc cctgtaatac agcgaaggat tgaaaaatgc
      481 caaaaaaggc caccgcggtt gatagcacag aacgaatttt aaaggaaaaa tcattactga
      541 aataaccgct gagccgggga gctgttttat gccagggagt tgaaaacaag gtctccagtt
      601 cggcttttac ctgggccggg gattcgttga gcttgtattt atacttagca cggaaaagaa
      661 gattcataga taaaatggta agcaattaaa tagaacaggc cggctaacta attttcaggc
      721 cttggaaaat atcctgcaaa tgtacaattc tccttacttc actagcttcc ttttatgcgg
      781 atactctgct tacaggtttt ttattttatc gagctccagc tggttgcttt cctgggtcag
      841 ccggcagata tcagtataaa accagttcgt aacgggttct tcccggatct tctttttgat
      901 cttttttttc cgctgtttga tgattaattt tttttctgtc caggcattgg catatacaat
      961 gtaatccttg cctgtttcaa attcatatcc gcaatcgcca tttccgtagc cagttacaat
     1021 acgaagggta tcagccaatc ctttatcact cttaaacctg ctctccacta ccagtgtata
     1081 gattttatac tgacgtatca tatacccgga agtggcccgg tcaaagggaa ttccggtaaa
     1141 caaggtgatt aaggtatcat aataaagata atcagttgaa gcaatgattt tacctttcac
     1201 gaccagcttc gatgaactga actcagactc cgttgtctgt ttgtctttac cgatacaggt
     1261 gcaggcataa ctactgcccg ttatcccggc ggtaaggacc agtaacaaca ttctgatgat
     1321 tggccggaag cgatataaca tagttgaaac aattcgaagt gtatattccg ggaggccgcc
     1381 gatcaaaact ccagcagcat ccggtgattc ggattgctat gagagggaga gatcgggaaa
     1441 tcgtgactgc cggtgatttt aaaccctttc ttttcataaa aacggatggc ccggatattt
     1501 tccttccaaa cgtaaagcca gactccggtt tgtttttcct tcttcatcag gtgcattaaa
     1561 aaatccatca gggccggtcc caggttggtg ccataataac ttttcaggag ataaatccgc
     1621 tccagtttgg tgatagcgtt gcccggactt ccctcatagg aggtattcaa taatatcttc
     1681 gaaaaaccca caggcaattt atgataatac aggatatgat aaatattggc cgggtcttcc
     1741 agttcggcct gcaaaatatc cggcgtataa ttttcggcta cataggcatc aatatctgcc
     1801 gccgccgcgc tatgcccatg tgattctata aaggtctgtg ttgctaatcc ggctaaaagg
     1861 gttgcgtcct taacctgcgc ttttacaatc gaaatcatgg ggcaaagtta atttataaaa
     1921 tgcgcagttc tcatttcttt atttcaaacc ggtattgccc cgatccggct ttaaccacga
     1981 ttttcccgtt ttcagttccc tgcagggtaa gatctttatg accggaaagc ggaactccgt
     2041 tttcagtaat agccgataaa tcggatgaag gaagatagat cagagcactg ctgtttaccg
     2101 gaatgcttat ttccaggatc agcaattgat tactgatttt ccagccactg acggcttctc
     2161 cataattcgt tttcagtctg gcttccagtt gagtaaaact tccaccggta tgcggttcaa
     2221 tccttaactg ctgatagccg ggggcttctt cacgggtatc cagtccggca gccacccggt
     2281 acatccagtc acctatggca ccataagcat agtgattaaa tgaattcatg gagggcacct
     2341 ggaaggtact gtccggtttt attccatccc aacgttccca gatcgtggtg gcgcccattt
     2401 taaccgggta caaccaggaa ggataggttt cctgtaataa taaacgataa gcgatatcgg
     2461 catagccaaa gcggctgagt acatgacaga gatagggcgt gcccaggaaa cccgtggtca
     2521 ggtgataacc ataactcttt atattttcca ccaatcgtgc ggcagcagtc tggcgcagtt
     2581 cttccggcag catatcaaat tgcagggcca gggtataggc ggtctgtgta ccggatacca
     2641 gtttcccatt agcggtcatg tattctttgc ggaaggcggc ttttattttt tctaaacgat
     2701 tccggtaatg atccgcatcg gctgtgttgc caaggaccgt ggcggttctc ctcatcaggt
     2761 ccgtggaatg cgcaaagaaa cattgggcga tcaggtattt atcggttacc gcggccctgc
     2821 catcattatc atcaaagggc cggtaaaata accagtcccc gaaatgaaaa ccggtggccc
     2881 ataaatcatc cttgctgttt ttttccataa aaccctgcca ggctttcatg ctgggatact
     2941 gatcttcgag tatttttttg tcaccatagg cgagatacat attccatgga atgatgctgg
     3001 cagcatcggc ccagccggta gatccggccg aacccgcacc caatacattg ggaatgacaa
     3061 agggcacact gccattttcc agctgatcag cggctacatc cttcatccat ttggcaaaaa
     3121 agttattgac gccaaaatta aaagccgctg tccgtgaaaa tacctgtgca tcaccggtcc
     3181 agcccaaccg ctcatcgcgt tgcggacaat cagtaggtac atccaggaag tttccttttt
     3241 gtccccattg aatattatgc tgcaactggt tgatgagttt attggaactg gtaaacgtac
     3301 cggtgggcgg catatcggaa taaagtgcca cagcggtcag gttatcggca gtcagttcac
     3361 ctggaaatcc ctccactttg atatagcgga aaccctggaa agt
//
LOCUS       DAOPMW010000375         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33643_length_3382_cov_7.797415, whole genome shotgun sequence.
ACCESSION   DAOPMW010000375 DAOPMW010000000
VERSION     DAOPMW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33643_length_3382_cov_7.797415"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLU11_13785"
     CDS             <1..682
                     /locus_tag="PLU11_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPN60251.1"
                     /translation="YYKLIAANLALYVFFAKKHRMTIGIIATTDQKDALLAQGFAAEA
                     RIEWMDRPAPLPGAVAVIDLMNRVEEYPAEWFESASTLLFINQVEKPIPENWPGHFIR
                     FNGWPGFWERSIVEATIKDASFQAKAESILAMLYKKTEWVADLPGFLSARILASIINE
                     AYLTFEEGVSSKEDIDTAMTLGTNYPLGPFAWCEKIGKSRIYILLNQLSANNQYQPAY
                     LLKNEAGF"
     gene            682..1350
                     /gene="tsaB"
                     /locus_tag="PLU11_13790"
     CDS             682..1350
                     /gene="tsaB"
                     /locus_tag="PLU11_13790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPN60252.1"
                     /translation="MALLLNIDTSQETASLCIARDEEIIGLKVNEQQHDHASWIHTAI
                     QALLKENGLVLSQIDAIAVTIGPGSYTGLRVGLATAKGLCFALSKPLIAIPTLEMMAF
                     AARFTTAALICPMIDARRMEVYAAIYDKNMNMVVSPEAVILEPTTYQSYITAHPVCFC
                     GSGSKKLQPLLQSQHAQFEDIPFSAMHMASISNRKFILQDFASLAYAEPLYIKEFYTA
                     ARRK"
     gene            1441..1779
                     /locus_tag="PLU11_13795"
     CDS             1441..1779
                     /locus_tag="PLU11_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPN60253.1"
                     /translation="MSTYTITLNDQKENGTLLKVDLLEVKKAALVLRALNHKLRQQIL
                     KMIDEGGKMTVTELYVKLRLEQSVASQHLAILRKAGFVKTDRDGKFIYYSVNINRMQE
                     LNQFVKQLLG"
     gene            1889..3298
                     /locus_tag="PLU11_13800"
     CDS             1889..3298
                     /locus_tag="PLU11_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPN60254.1"
                     /translation="MFIKQLYTSCLSEAAYYIEDNGEAAVIDPLRDIEAYLQLAHERN
                     ARIKFIFETHFHADFVSGHLDLGQATGAPIVYGPGTTTKFPVHVAKDGEKFNVGNMEI
                     EVLHTPGHTLESSCYLLRDAAGKAHCVFTGDTLFVGDVGRPDLAQKGAELTTTDLAGM
                     LYESIHQKLFPLADDVIIYPAHGPGSSCGKNLGPETQSTIGVEKQQNYAMKAPTKEDF
                     IKQVTEGITAPPSYFPVNARINKEGYDSLEKVVNKGMQALSPAAFNEKITDDLLILDT
                     RHANIFMNGYVPGAISIGLEGRFAEWAGSLLPFDKPMLLVTEAGKEKESIIRLARVGF
                     DKIEGHLSGGFEAWQEAGLPIDMIIDVEADELMMDMPHDPNLVVVDVRRETEFADGHL
                     KDAINIPLNEMTDPGSIANIEDHHNLYVHCAGGYRSVIAASLLKRQGIHNLRNINGGW
                     AKIKEEKKAEIVKESSVLN"
BASE COUNT          974 a          724 c          784 g          900 t
ORIGIN      
        1 atattataaa ttgattgcag cgaatttagc tttatacgtt ttctttgcaa aaaaacaccg
       61 gatgaccatc gggataatcg caactactga tcaaaaagat gccttgcttg cgcagggatt
      121 tgcagcggaa gcccgtattg aatggatgga ccgtcctgcc ccattgccgg gagcagtcgc
      181 tgtgatagat ttgatgaacc gggtggaaga atacccggct gaatggttcg aatctgcgtc
      241 tactctccta tttatcaatc aggtggaaaa accgattccg gaaaactggc ctggacattt
      301 tatccgtttt aacggttggc cgggcttttg ggaacgttca attgtggaag caaccattaa
      361 agatgcctcc tttcaggcaa aggcagaatc aatactagct atgttataca aaaaaacaga
      421 atgggtcgct gatcttccgg gatttctgtc tgcccgtata ctggcttcca tcattaatga
      481 agcctattta actttcgaag aaggagtgag cagtaaagaa gatattgaca cagcgatgac
      541 attagggacc aactaccctc ttggtccttt tgcctggtgt gaaaaaattg ggaaaagcag
      601 gatttatata ttgttgaatc aactatcagc aaacaatcaa taccagcccg cttatttatt
      661 aaaaaatgaa gccggttttt aatggcccta ctattaaata tagacacctc acaggaaact
      721 gcctctctct gcattgcccg ggatgaagag ataataggtc tgaaagtcaa tgagcagcaa
      781 catgatcatg catcctggat tcatactgct atacaggcat tactaaagga aaacggactc
      841 gttttgtcac aaattgatgc catcgccgtt actattggtc ccggatctta tactggttta
      901 agggtcggac tggccaccgc gaaagggttg tgtttcgccc tcagcaagcc gctgattgcg
      961 attcctaccc ttgaaatgat ggcattcgcc gcccgtttta ctactgctgc gttaatctgt
     1021 cccatgatcg atgcacgacg gatggaagtg tatgcagcga tatatgataa aaatatgaat
     1081 atggtggttt cgccggaagc tgtgatactc gaacccacca cttatcagtc atatattacc
     1141 gctcatcctg tttgtttctg cggaagtggc agcaaaaaac tgcaaccact tttacagtct
     1201 caacatgcac aatttgaaga cattccgttt tcggctatgc atatggcatc catttcaaac
     1261 cgaaagttca ttttgcagga ttttgcaagc cttgcctatg cagaaccctt atatatcaaa
     1321 gaattttata ctgctgcccg aaggaaatag atatagttca cgtcttatca tttaattact
     1381 taatacctga catccggaag gtatttttgt ttcttttttc caaccaaccc gttcacagat
     1441 atgagtacgt atacaattac actaaatgac caaaaagaga atggaaccct tttaaaagtg
     1501 gaccttcttg aagtaaaaaa agcagccctg gttttgagag ctttaaatca taagttacga
     1561 cagcagattc tgaagatgat tgatgaaggt gggaaaatga cggtcacgga attgtatgta
     1621 aaactccgtc ttgaacaatc ggttgcttcc cagcacctgg cgattcttcg taaggctggt
     1681 tttgtgaaaa cggaccgtga cggaaaattc atttactact cagtgaatat caaccggatg
     1741 caggagctca atcaattcgt gaaacaatta ctgggataac cacattgcta aaaacgttga
     1801 aaagccccgt tctctgtcaa aagagacggg gcttttcata ttataaagta attttgcatt
     1861 tttccggaaa ttcaaaaacc acgaatctat gtttattaag caattatata cttcctgtct
     1921 gagtgaggcg gcctattata ttgaagataa cggtgaagcg gccgtgatag atccgcttcg
     1981 tgatatcgaa gcgtacctgc aactggctca tgaacgcaat gcccgcatca aattcatttt
     2041 tgaaactcat ttccacgccg actttgtgag cggccatctg gatctcggcc aggctaccgg
     2101 cgccccgatt gtatatggtc ccggcactac aacaaagttc ccggttcatg tggcaaagga
     2161 tggtgaaaaa ttcaacgtgg ggaacatgga aatagaagtc ttacatacac ccggccacac
     2221 gcttgaatcc agttgttatt tattaaggga tgcggctggt aaagcgcatt gcgtttttac
     2281 cggtgatact ttatttgtag gagatgtggg tcgtccggac ctggcacaaa aaggagcaga
     2341 actgaccact actgaccttg caggtatgtt gtacgaaagt attcaccaga aattgttccc
     2401 gctggcggat gacgtgatca tttatccggc acatggaccg ggcagttcct gcggtaaaaa
     2461 cctcggtccg gaaacacaga gcacgattgg cgttgagaag caacagaatt atgccatgaa
     2521 ggcgccaacc aaggaagatt ttattaaaca ggttacagaa ggcataaccg cccctccctc
     2581 ctatttcccg gtcaatgcac gtatcaataa agaaggttat gacagcctgg aaaaagtagt
     2641 gaataaaggg atgcaggcgt tgagtccggc tgcattcaat gaaaagatta cagacgatct
     2701 gctcatcctt gacactcgtc atgcaaatat atttatgaac gggtatgtac caggtgccat
     2761 cagcatcgga ctggaaggtc ggtttgccga atgggccggc agcctgcttc cctttgataa
     2821 accgatgcta ctggtaactg aggccggtaa agaaaaagaa tcgatcatcc gtctggcccg
     2881 ggtaggcttt gataaaattg aagggcattt atccggcggc tttgaagcct ggcaggaagc
     2941 gggtttaccc attgacatga tcattgatgt ggaagccgat gaactgatga tggatatgcc
     3001 gcatgatccc aacctggtgg tggtggatgt acgtcgcgaa actgaatttg ccgatggaca
     3061 tttaaaagat gccatcaata ttcccctgaa tgaaatgacc gatcccgggt ccattgctaa
     3121 tatcgaagac caccataatc tgtatgtgca ttgtgccggc gggtaccgca gtgtaatcgc
     3181 tgcttcctta ctcaaaagac aaggcatcca taacctgcgt aatatcaatg gtggctgggc
     3241 gaagatcaag gaagagaaga aggcggagat tgtgaaggag agtagtgtgc ttaactagtt
     3301 agtagatagt agtcggtagt tagtagtcaa cagcccgttc taagttagat atccttgatc
     3361 cgtgaatacc tgcctgccgg ca
//
LOCUS       DAOPMW010000376         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33693_length_3378_cov_6.211556, whole genome shotgun sequence.
ACCESSION   DAOPMW010000376 DAOPMW010000000
VERSION     DAOPMW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33693_length_3378_cov_6.211556"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="murB"
                     /locus_tag="PLU11_13805"
     CDS             complement(<1..865)
                     /gene="murB"
                     /locus_tag="PLU11_13805"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPN60255.1"
                     /translation="MKPEENIALAPFTSFGVESTARYYSTFRDEAELEELLFWGARYP
                     LLILGGGSNLLLTRDVDGLVLKNEIRGITELHEDNDYVYIRAGAGENWHQFVLYCLSR
                     NWAGLENLSLIPGNAGASPMQNIGAYGVELEELFWDLEAWHIREQKKVTFTKSDCGFG
                     YRDSVFKGKFKGQFVILNVTYRLRKKPRYTTSYGAIEKELEKMGVKELSIKAISDAVI
                     SIRSSKLPDPRVIGNAGSFFKNPSVSAEKHALLQKQFPDLVAYPNVDGTFKLAAGWMI
                     EQSGPAPGISWK"
     gene            1033..2397
                     /locus_tag="PLU11_13810"
     CDS             1033..2397
                     /locus_tag="PLU11_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN60256.1"
                     /translation="MKKYFLLLLLPAVFIFSCKSRNDKPESAATAGNGSHPDWIMQGN
                     IYEVNTRQYTPEGTFNAFAAHLDRLKEMGVQTVWFMPINPISKKDRKGTLGSYYAVAD
                     YTGINPEYGTLDDWKALVKKIHEKEMKVIIDWVPNHTGADHPWLKTHPDFFVKDSTGQ
                     AATMFDWTDTRVLDFKNNVMQDSMIAAMKYWITETGIDGYRVDVAWNVPGEFWKKCIP
                     QLKAVNKDLFFLAEGDKAYLMTSGFDAYYPWEMFHMMVKIAAGNRPAFGLDSIKAKYD
                     TTYPKEAIPMYFTSNHDENSWNKSDYATFPGVSHAPFAVFTQTMSTTVPLVYSGQEEP
                     LLRPLPFFEKDSLVFSKLERAKFYTTLQKLRTANPALAADAAFRKVKAGDEKAVYAYV
                     REKAGKKILVILNLSKTEQAIQIADKTLHGKPYNVFMGTTEPLGDTEWKMQPWGYVIY
                     EYKD"
     gene            2425..3036
                     /locus_tag="PLU11_13815"
     CDS             2425..3036
                     /locus_tag="PLU11_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPN60257.1"
                     /translation="MHATIIGSTGLIGGHLLDLLINDPFFKPVRILIRRPLDISHPKL
                     EKKLVDFSDIESFRLALEGTDVIFCCIGTTMKKMKGDKTAYRKIDYDIAVHAAKLGKA
                     NGCEKFVLVSAVGADSKSRNFYLKLKGEIEEAIQATGIESVYILRPSLLMGNRKEFRA
                     GEKFASWLMPLFAFLLPARYKPVKASEVAKAMVNAVKEPVLKN"
     gene            3130..>3378
                     /locus_tag="PLU11_13820"
     CDS             3130..>3378
                     /locus_tag="PLU11_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60258.1"
                     /translation="MRKTFILVIAGLLFCNALSAQKEKKSWFGVKGGFNHTVINGVET
                     DGDKTGFVGSTLYGGLFTENPIGATTLLMNELIFSWVND"
BASE COUNT          956 a          787 c          738 g          897 t
ORIGIN      
        1 ccttccagct gattccgggt gcgggtcctg attgttcaat catccaaccg gctgccagtt
       61 tgaaggtccc atctacattg ggataagcca ccaggtccgg gaattgtttt tgcaaaagag
      121 catgcttttc tgcactgact gatgggttct taaaaaaact gccggcattt ccgattaccc
      181 ggggatcagg taatttggaa gaccggatac tgattaccgc atctgatatt gcttttatgg
      241 aaagttcttt cacccccatt ttttccagtt ccttctcaat agccccataa ctggtagtat
      301 accggggctt tttcctcaac cggtaggtta cattcagtat tacaaactgt cctttaaatt
      361 ttcccttgaa cacactatcc cggtagccaa atccacaatc actctttgta aatgtgacct
      421 ttttctgctc ccggatatgc caggcttcca ggtcccagaa cagctcttcc agttccactc
      481 cgtatgcgcc gatattctgc attggagaag cgcctgcatt tcctggaatc agggaaagat
      541 tctccagtcc tgcccagtta cgactaagac agtataacac aaactggtgc cagttttctc
      601 cggctcccgc ccggatatag acataatcat tgtcttcgtg caattcggta attcccctta
      661 tttcattttt taacaccaac ccgtccacat cccgggtcag caacaggttg cttcctcctc
      721 ccaaaatcaa taaaggatac cgggctcccc aaaataaaag ttcttctaac tccgcttcat
      781 ccctgaatgt ggaataatag cgggccgtgc tctccacccc aaaactggta aatggggcca
      841 gggctatatt ttcttccggt ttcatcaact gcaatttaat catgaaaatt acaaactcag
      901 gagctggaga aaaaataaac gcctgatttt catttttaaa aggggttgta ctgaacagcc
      961 ccatttttgc tgtccggcca taatggacta atttcactgt ccggtttctg tcacttatta
     1021 aaaaataagg ttatgaaaaa atacttcctg cttcttttac tccccgctgt ttttattttt
     1081 tcctgcaaat ccaggaatga taaacctgaa tcggctgcta ccgcaggtaa tggatcgcat
     1141 cccgactgga tcatgcaggg caatatctac gaagtcaata cccgccagta tactcccgaa
     1201 ggaacattca acgcttttgc tgcacatctc gaccggttaa aagaaatggg cgtgcaaacc
     1261 gtgtggttta tgccgatcaa cccgatcagt aaaaaagacc gtaaaggaac gctgggcagt
     1321 tattatgcag tggcggatta tactggtatc aatccggagt atggcactct ggatgactgg
     1381 aaagcgttgg ttaaaaaaat tcacgagaag gaaatgaaag taatcatcga ctgggttccc
     1441 aaccatacgg gtgctgatca tccctggctg aaaacgcatc ctgatttttt tgtaaaagac
     1501 agtaccggtc aggcagccac catgtttgac tggacagaca cccgcgtgct ggattttaaa
     1561 aacaacgtca tgcaggacag catgattgcc gcgatgaaat actggataac cgaaaccggc
     1621 atagacggtt accgggtgga tgtggcctgg aatgtacctg gcgaattctg gaaaaaatgt
     1681 atcccgcagc taaaggcggt caataaagat ttattcttcc tggcagaagg cgacaaagct
     1741 tacctgatga ccagtggttt tgatgcttac tatccctggg aaatgtttca tatgatggtt
     1801 aaaatagctg ctggcaaccg acctgcattc ggactggaca gtatcaaggc taaatatgat
     1861 accacttatc ccaaagaagc gatccccatg tattttacca gtaatcatga tgaaaatagc
     1921 tggaataaat cggattacgc cactttcccg ggagtttctc atgctccatt tgccgtgttc
     1981 acgcaaacca tgagtaccac cgtcccgctc gtatatagcg gtcaggaaga acccctgctc
     2041 cgtcccctgc cattctttga aaaagacagc cttgttttca gtaaactgga gcgggctaaa
     2101 ttttatacga ccctgcaaaa acttcggacg gctaatcccg cattggcagc cgacgccgcg
     2161 ttcaggaaag taaaagcagg agatgaaaaa gcggtctacg cttatgtaag ggaaaaagca
     2221 ggaaaaaaga tcctggtcat cctgaatctt tccaaaacag aacaagccat tcaaatcgct
     2281 gataaaacat tacacggcaa accctataat gttttcatgg gaactacgga acccttgggt
     2341 gatacggagt ggaaaatgca accatggggt tatgtaattt atgagtacaa agactgataa
     2401 ccgcttttgt aaaccctgtt cgttatgcac gcaaccatta tcggttccac cggacttatt
     2461 ggcggccact tgcttgatct gctgatcaac gatccctttt ttaaacccgt taggattttg
     2521 atcaggcggc cattggacat cagccatccg aaactggaaa aaaagctggt ggacttcagt
     2581 gatatagaaa gttttcgcct ggccctcgaa ggaaccgatg tgatcttctg ttgcatcggt
     2641 accacgatga agaaaatgaa aggggataaa accgcttacc ggaaaataga ctatgatatt
     2701 gccgtacatg cggcaaaact gggcaaggcc aacggctgtg aaaaatttgt cctggtgtct
     2761 gcagttggtg ccgacagtaa aagccggaat ttctacttga aattaaaagg tgaaattgaa
     2821 gaagccattc aggcgaccgg aattgaatcg gtatatattt taaggccttc ccttcttatg
     2881 ggtaaccgca aagaattcag agcaggtgaa aagttcgctt cctggctgat gcccttgttt
     2941 gctttcctgt tacctgccag gtacaaacca gtgaaagcca gtgaagtggc taaggcgatg
     3001 gtgaatgcgg tgaaagaacc ggtcctcaaa aattgattcc ctgtaaacca gttataatat
     3061 aattcaatga aattcgtttg attatacaac cgttgcgggc accggcccca aaaacaataa
     3121 tctaaacaca tgagaaagac tttcatcctg gtcattgcag gactcttgtt ttgcaacgcc
     3181 ctttctgcgc aaaaagaaaa aaaatcctgg tttggggtta aaggtggatt caatcatacc
     3241 gttattaacg gggttgaaac ggatggagac aaaaccggtt ttgtgggttc cactttgtat
     3301 ggaggtcttt tcacggagaa ccctatcgga gctaccacct tactgatgaa tgaacttata
     3361 ttctcctggg tgaatgac
//
LOCUS       DAOPMW010000377         3371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33793_length_3371_cov_6.462002, whole genome shotgun sequence.
ACCESSION   DAOPMW010000377 DAOPMW010000000
VERSION     DAOPMW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33793_length_3371_cov_6.462002"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1310
                     /locus_tag="PLU11_13825"
     CDS             72..1310
                     /locus_tag="PLU11_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPN60259.1"
                     /translation="MKHPKELSILDYTYPLPETRIAAFPLAERDASKLLIYEKGNIRT
                     DTYHQLADYIPADSLLVFNNTKVVEARLLFQKNTGGVIEVFCLEPHEQYADITSAMLQ
                     QGKVQWQCLVGGASKWKPGQVLEKKIPYNGNEIILQAAYMEKRAGCFIIELSWTPTTM
                     SFAELLHAAGAIPLPPYIKRKAESSDTERYQTVYAKHDGSVAAPTAGLHFTEQLFQSL
                     KGKNVRHDYVTLHVGAGTFKPVKSEKMVEHDMHAEWIDVSRSFIETLLKSLSGNVITV
                     GTTSLRTVESLYWIGRKAASGELRVTSIEITQWEVYKRTENELSAKEALSSLLVWMKE
                     HNLERLVTKTQILIAPGYQFRIINGLVTNFHQPQSTLLLLVAALIGDDWRNVYNYALE
                     NEFRFLSYGDGSLLWSGKEV"
     gene            complement(1522..2535)
                     /locus_tag="PLU11_13830"
     CDS             complement(1522..2535)
                     /locus_tag="PLU11_13830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN60260.1"
                     /translation="MTYLKILLASLFLYLSASGQTEIQLVLKNPENHHIDKVDAFDLS
                     QVEIYKYKFKDTLLLRFKKNNIDCYNIRYHENGKMYRQQIWLDPGKITIKAHIDGDNL
                     VIDTVLNSPFYYQVADFNEAYRSFLTTRDSTGMNTFLLNSFEENSNNPFSMNVGLYFL
                     TLNQNKKDQLQKLRTLIDGQAGRFNWFLLFPMVSGRLDKLLTVNRIDFAKFSFINEYK
                     RKTAIKLDGAEFYVIDCWFLACMPCIAQHKEIKENLDKLRSRKVEVIGVSTDRDWKKW
                     KTYLTDHNYTWANFTEDKSLAFTKELSIAAFPTYLILNKDGEILESFNSFKEVLMRFQ
                     IVE"
     gene            complement(2591..>3371)
                     /locus_tag="PLU11_13835"
     CDS             complement(2591..>3371)
                     /locus_tag="PLU11_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN60261.1"
                     /translation="ATSVLFTIIILAAFYLTVRTMLRQKKLGEIKNDFINNMTHEFKT
                     PIATISLAVDAMRNDKVISDKDKLRYFSGIIKEENQRMNRQVETILKASQLEKQEVDL
                     NLKPVHVHEIIKDVVGNFALQLESKNGKVDLLLNATNDLVEADEVHFSNLVNNLVDNA
                     VKYARDNNPPQIRISTQSGSKYFNLRIEDNGIGMNRDTLKRIFERFYRAHTGNMHNVK
                     GFGLGLSYVKTMVEAHHGEIKAESTLGKGSVFTIDIPLKRG"
BASE COUNT          930 a          750 c          728 g          963 t
ORIGIN      
        1 cagtacgatc agctactcat tactgattac tcattactca ttatcttcac catacaacag
       61 ttggttgtgt catgaaacat cccaaagaac tttctattct cgactacacg tatccgttac
      121 ctgaaacccg tatagccgca ttcccattgg cagagcggga tgcttctaag ttgctgatct
      181 atgagaaagg aaatatccgt acagatactt accatcaact cgctgattat attcctgctg
      241 attcattact ggtattcaac aataccaaag tggtggaagc ccgtctgctc tttcagaaaa
      301 atacaggtgg ggtgattgaa gtgttttgtc tcgaacctca tgagcaatat gcggatatca
      361 caagtgccat gttgcagcaa gggaaagtac aatggcaatg cctggtgggt ggcgcctcca
      421 aatggaaacc gggacaggtg ctggagaaaa aaatcccgta taacggtaat gagatcattc
      481 tgcaggctgc ctatatggag aaacgtgccg gctgttttat cattgaactc agctggacgc
      541 caaccactat gagttttgcc gaactgctgc atgcagccgg ggcgattccc ctcccccctt
      601 atatcaaacg taaagctgag agcagtgata ccgaaagata ccagaccgta tatgcaaagc
      661 atgacggatc cgtagcagcg ccgacagccg gtctgcattt cactgaacaa ctattccagt
      721 cactgaaggg aaaaaatgtc cgccatgatt atgtaaccct gcatgtgggt gccggcacct
      781 tcaaaccggt gaagtcggag aagatggttg aacatgatat gcacgcggag tggatcgatg
      841 tgagccggtc ctttattgaa acattgttga agtccttaag cggaaatgtc attacggtcg
      901 ggactacttc tttaagaacg gtggagagtc tgtactggat cgggcggaaa gctgcgagcg
      961 gcgagttacg agttacgagt atagagataa cccaatggga agtgtataaa cgaacagaaa
     1021 atgagttatc agcgaaggaa gcattgagtt cattgctggt atggatgaag gaacataacc
     1081 ttgaaagatt agtgacgaaa acacagatcc tgatcgctcc cggttatcaa ttccggatca
     1141 tcaatgggct ggttactaat tttcatcagc cgcaatccac cttgttattg ctggtagcgg
     1201 cattgattgg cgatgactgg cgaaacgtgt acaattatgc gctggaaaat gagttccggt
     1261 ttctgagtta tggggacggt agtttgttgt ggagcgggaa ggaggtttag aggtttggtc
     1321 gtttggaata ctcttcttag gagagtttgt tgttttactt ccatcaaaaa gcgaagagag
     1381 gaacctgcca tccggtaaga aacgccgtcc attattgagc tactacattc aataatcata
     1441 cacaatagca aaacaacctc acctgaatgc cgatccacgt ttcccggtta cccaccagaa
     1501 aaccgccaaa ctttaccgta cctactcaac gatctgaaat ctcatgagta cttccttaaa
     1561 ggaattgaat gattccagaa tttccccgtc tttatttaat ataaggtatg taggaaaagc
     1621 cgcgatactc agctcttttg taaaagccag tgatttatcc tccgtaaagt tggcccaggt
     1681 ataattatga tccgtcaaat aagtcttcca tttcttccag tccctgtcag ttgacacccc
     1741 gataacttca actttcctgc tcctcagttt atctaaattt tcctttatct ctttatgctg
     1801 tgcaatacag ggcatacaag ccaaaaacca gcaatcaatt acataaaatt cagccccgtc
     1861 aagtttgatc gcagttttcc ttttatactc attaatgaat gagaactttg caaagtcaat
     1921 tctgtttacg gttaacagtt tgtcaagacg gcccgatacc atcggaaaaa gcaggaacca
     1981 gttaaaccgg cctgcctggc catcaatcag tgttcttagc ttttgcagct gatccttttt
     2041 attctggttc aatgtcagaa aatagagtcc tacattcatt gaaaaaggat tgttgctgtt
     2101 ttcctcaaaa ctgtttagca aaaaagtatt catcccggta ctgtcccggg ttgtcaagaa
     2161 agatctgtat gcttcattaa aatcagcgac ctggtaataa aacggagaat tcagtacggt
     2221 atcaatgacc agattgtcac cgtcaatatg ggctttgatt gtgattttcc cgggatcaag
     2281 ccatatctgc tgtctataca ttttcccatt ctcatgatac cggatattat aacagtcaat
     2341 attgttcttt ttgaatctga gtaacaatgt atccttaaac ttatacttat aaatttccac
     2401 ctggctcaaa tcaaaagcgt ccactttatc gatgtgatga ttctccggat tttttaaaac
     2461 cagctgaatt tcagtttgcc cggaagcaga caggtataga aataaagaag caagaagtat
     2521 tttaaggtaa gtcatacatg cagcattaca gggttagcct tcattttttg attcaggcaa
     2581 taacaatccc ctatcctctc ttcaatggaa tatcaatcgt aaacacactt cccttcccca
     2641 gtgtactctc cgctttaatc tccccgtgat gggcttccac catggttttt acatagctga
     2701 ggccgagacc gaagcctttt acattgtgca tattaccggt atgggcccgg tagaaacgtt
     2761 cgaagatgcg ttttaaggta tctcggttca taccgatacc attgtcttcg atacggaggt
     2821 tgaagtattt actgccagac tgggtactga tccggatttg tggcgggttg ttatcgcggg
     2881 catatttcac cgcattatcg accagattat tgaccaggtt ggaaaaatgc acttcatcag
     2941 cctccaccag gtcattggtg gcattaagca acaggtccac tttaccgttc ttactttcca
     3001 gctgaagggc aaaatttccc accacatctt tgatgatctc atgcacgtgg accggtttga
     3061 gattcaggtc cacttcctgt ttttccagtt gcgaggcttt caggatggtt tccacctgcc
     3121 ggttcatccg ctgattctct tccttgataa tcccgctgaa ataacggagt ttatccttgt
     3181 cactgatcac tttatcattc cgcatggcat ccacggccag cgagatggtg gcaatcggtg
     3241 ttttaaactc atgcgtcatg ttattgataa agtcattctt gatctcgccc agttttttct
     3301 gtcgcaacat ggtgcgaacg gtaaggtaaa aggcggccag gatgatgatg gtaaacagta
     3361 cagaagtggc a
//
LOCUS       DAOPMW010000378         3366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33870_length_3366_cov_6.056176, whole genome shotgun sequence.
ACCESSION   DAOPMW010000378 DAOPMW010000000
VERSION     DAOPMW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33870_length_3366_cov_6.056176"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLU11_13840"
     CDS             complement(<1..978)
                     /locus_tag="PLU11_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60262.1"
                     /translation="MKNRIKKILIAAVWLLSGTVYAQRDTILLNTGWVFSIDKKGEGV
                     SKKWFAEPLPYARSVQLPHTWNIEAQSENHYGWGWYQRKLEIPVNWKNKNVVVRFGAI
                     NHSSFIYLNGEKIGEHIGDGLNAFTLNLAGKLKYGKENSITIAVNNDYGRNKVPFGSS
                     FDWPNDGGLIRKVMLIVSDRPAADYIRVTPALNVKTHEGKLKIKLGFTGLKDSSQLQL
                     AITITEENQQTQQTIYQRTQKPGWQNNEAVVDIVLPKINPWHFDRPDLYRIDVRVVSG
                     KKTSDHISTSTGFREILFAGGKTWLNGEYIRLMGVEWTAGSNPDYGFAET"
     gene            complement(987..2153)
                     /locus_tag="PLU11_13845"
     CDS             complement(987..2153)
                     /locus_tag="PLU11_13845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPN60263.1"
                     /translation="MNKIVLLYSLSLFFLLSVAAQEKIPVFVSGTEGHKSYRIPAIIK
                     NPNGDLLAFCEGRVNGSGDFGDINIVMKKSSDQGKTWTKLETIVDADSLQAGNPAPVV
                     DLNDPVYPGGRIFLFYNTGNNHEGEVRKGKGLREVWYKTSSDGGLTWSAAVNITTQTH
                     RPKQPQVNAAYKFLEDWRSYANTPGHALQLTGGQYKGRIYVAANHSEGAPKGRFEDYF
                     AHGFYTDDHGQTFRLSETIQLPGGNEATAAEISNNGLLLNARNQKGDVRARIVARSND
                     GGQTWKSIDFDKNLPDPVCEGSILNIGVKKRKHLLAFCNAADTAKRNNLTLRISYDEG
                     HTWAKSIPLDKSEDGKKDFTAYSDIVKVSKKEIGVLYERDGYSQIVFTLVNWKK"
     gene            complement(2150..>3366)
                     /locus_tag="PLU11_13850"
     CDS             complement(2150..>3366)
                     /locus_tag="PLU11_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPN60264.1"
                     /translation="NNILIGDIWICLGQSNMEWPMIREKHYPEEIKNSLQPLLRFYNP
                     TYAGKNIFGNAFPDSVAALLTPRRFYKGYWQACDSSSIKSMSAVAWYFGKQLAAELNI
                     PVGLINLSIGGAPLETFIDVNTLLSSKQFAAKVKGDWLKNNALPVWIRERGKQNIENI
                     PDAPVDEYGKNHAFKPGFAFESGIKSLLPMPVKGIICYQGESNAQEMERVMEYGALTA
                     LMVNDYRKKWGKPGLPFYFVQLSSMDSANYKSQLWPLFRDEQRNLLQLIPNTGMAVSS
                     DIGAKNDVHPTNKKAVGERLARWALNKIYHKKMLPSGPLPLKARYENGKVTITFNYAG
                     SRLLTADGTIIRGFSTDGNTDCNAFIENDTVIIPAEARPAYVYYGWKPFTNANLINAE
                     GLPASTFKIKVQ"
BASE COUNT          786 a          817 c          729 g         1034 t
ORIGIN      
        1 ggtttctgca aaaccataat ccggattgct gccggcggtc cattcgaccc ccatcagcct
       61 gatatactca ccattcagcc aggtttttcc cccggcaaaa aggatttccc tgaatccggt
      121 gctggtggaa atatgatcac ttgttttttt tccggatact acacgcacgt caatacggta
      181 aagatccggt cggtcaaaat gccaggggtt tattttgggc aatacgatat caacaaccgc
      241 ttcattgttt tgccagcctg gtttttgtgt tcgttggtaa atagtctgct gtgtctgctg
      301 gttttcctcc gttatggtaa tggccaattg caattggctg ctgtctttca gcccggtaaa
      361 gcccaatttg attttcaact taccttcatg cgttttgaca ttcaatgccg gtgtgacacg
      421 aatatagtcc gcagcgggcc ggtcactgac aatgagcatg actttacgga tcaggccacc
      481 atcgttgggc cagtcgaatg aactgccaaa aggaactttg tttcgcccat agtcattatt
      541 caccgctatg gtaatgctgt tttcttttcc atatttcagt ttgccagcta aattcaacgt
      601 gaatgcgttc aagccgtcgc cgatatgctc cccgatcttt tccccattga gatagatgaa
      661 cgaactgtga ttgatggcgc caaaccgcac taccacattt ttatttttcc agttgaccgg
      721 gatctccagt tttcgctgat accaacccca tccgtaatga ttttcgcttt gggcttcaat
      781 attccaggta tggggaagct gcacggatct ggcgtagggc aggggttctg caaaccattt
      841 tttgctgacg ccttctcctt ttttatcaat actaaaaacc catccggtat ttaataagat
      901 tgtatcccgt tgggcataaa cggttcctga aagcaaccaa acagcggcaa tcagtatttt
      961 ttttatccgg tttttcattc gcatggttat ttcttccagt tgaccagggt gaaaacaatc
     1021 tgcgagtagc catccctttc atataaaacg cctatttctt ttttactgac cttaacgata
     1081 tccgagtaag ccgtaaagtc tttctttcca tcctccgatt tatccagcgg tatacttttg
     1141 gcccaggtgt ggccctcatc ataactgata cgcagggtca ggttgtttct ttttgcggta
     1201 tccgccgcat tgcaaaaagc aagcaggtgt ttccgttttt taacacctat attcaggata
     1261 ctgccttcgc agaccgggtc aggcagattt ttatcaaagt ctatgctctt ccaggtttgt
     1321 cccccatcat tgcttctggc cactatcctg gcccgtacat cccctttttg attccgcgca
     1381 ttcagcagta atccgttatt ggagatttca gcagcggtgg cttcgttgcc cccgggtaat
     1441 tgaatggttt cgcttaaacg aaaggtttgg ccatgatcat ccgtataaaa gccatgggca
     1501 aagtaatctt caaaacgacc tttaggggca ccctcagagt gattggctgc aacatatatc
     1561 cgtcctttgt attgaccgcc tgtcaactgc agtgcatggc cgggtgtatt ggcataactc
     1621 cgccagtctt ctaaaaattt ataggcggca ttcacctggg gttgtttcgg acgatgtgtc
     1681 tgggtggtga tatttacagc cgcgctccag gttaagcccc cgtctgagga ggttttatac
     1741 catacttcac gcaagccttt tcctttcctg acttctcctt catgattatt gccggtatta
     1801 taaaacagga aaattcttcc acccggataa accggatcgt tcaggtcaac gacaggagcc
     1861 ggatttccag cctgaagact gtctgcatct acgatggttt ccagttttgt ccaggttttt
     1921 ccttggtcac tgcttttttt catgacaata ttgatatccc cgaagtcgcc acttccgttt
     1981 acccggcctt cacaaaacgc cagcagatca ccattgggat tttttatgat ggcgggtatc
     2041 cggtaacttt tatgtccttc ggtgccggat acgaataccg gaattttttc ctgtgctgca
     2101 acagaaagaa gaaagaaaag gctcagggag taaagcaata ctatcttgtt cattgtactt
     2161 taattttaaa ggtggaggcc ggcagccctt cagcgtttat aagattggca ttggtaaagg
     2221 gtttccagcc ataatacaca taagcagggc gggcttcagc cgggatgatc actgtgtcgt
     2281 tttcaatgaa tgcattacag tccgtattac cgtctgttga aaagcctctt attatagttc
     2341 catcggcggt cagcaaacgg cttcccgcat aattaaaagt gatggtaacc ttaccgtttt
     2401 cataccttgc tttcaaaggt aatggaccgg aaggaagcat ttttttatga tagattttat
     2461 tcagtgccca gcgggcgagc cgttcaccca ccgctttttt atttgtcggg tgcacgtcgt
     2521 ttttcgcgcc aatatcgctg ctcaccgcca tgccggtatt gggaatcagt tgcagcaggt
     2581 ttcgctgttc atccctgaac aagggccata actgactttt atagttggcc gaatccatgg
     2641 atgaaagctg tacaaaataa aatggcagcc ctggctttcc ccattttttc cggtaatcat
     2701 tcaccattaa tgcggttaat gcgccatatt ccattaccct ttccatttcc tgggcattgc
     2761 tttctccctg gtaacagatg attcctttta ccggcatcgg caataaggat tttatgcctg
     2821 attcaaaggc aaatccgggt ttgaatgcat gattctttcc atattcatca accggtgcat
     2881 cggggatgtt ttcgatattc tgcttccctc tttctcttat ccataccggt aatgcattgt
     2941 tcttaagcca gtcacctttt actttagccg caaattgttt gctggaaagc agggtattca
     3001 catcaatgaa tgtttcaagc ggcgccccgc cgatggaaag attgatcagg cccacgggta
     3061 tattcagttc agccgctaac tgtttgccga aataccaggc taccgcactc attgatttga
     3121 tactgctgct atcacaggcc tgccagtatc ctttgtagaa acgcctgggg gtaagcagtg
     3181 cagccacaga atcagggaag gcgtttccga aaatattctt accggcataa gtgggattat
     3241 aaaaacggag cagtggctga agactgttct ttatctcttc cgggtaatgt ttctcccgga
     3301 tcatcggcca ttccatatta ctttgtccga ggcagatcca gatatcacct atcaggatat
     3361 tgttaa
//
LOCUS       DAOPMW010000379         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33917_length_3362_cov_9.213789, whole genome shotgun sequence.
ACCESSION   DAOPMW010000379 DAOPMW010000000
VERSION     DAOPMW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33917_length_3362_cov_9.213789"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1358)
                     /locus_tag="PLU11_13855"
     CDS             complement(108..1358)
                     /locus_tag="PLU11_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN60265.1"
                     /translation="MKHLVILGAGTAGTMMANHLSHHLKRPEWRISIIDERTEHHYQP
                     GYLFLPFDIYEPEDIVKSIQEFIPKTVDLIQASIDRIVPAENKVMMTDGSVVVYDVMI
                     VATGAKIAPEETEGMMGSEWHQSVFDFYTFEGALNLRNKLRDWKGGKLVVHITEMPIK
                     CPVAPLEFAFLADSFFKNKHMRDKVEITYVTPLSGAFTKPKATATLEHLLEEKHINIE
                     SDFAIEQVDNENKKIIDYGGREIPFDILVTVPTNKGDAVMERSGMGDDLNYVPTNKAT
                     LQSKAYQNVFVLGDASNIPASKAGSVAHFEAEILTENILRFVKGEPLKEEFDGHANCF
                     IETGGGKALLIDFNYTHEPVEGTFPFPGLGPLRLLKESRMNHMGKLAFRWVYWHMLLK
                     GTHIPFVSATMSESGKHYEKTETV"
     gene            1668..2141
                     /locus_tag="PLU11_13860"
     CDS             1668..2141
                     /locus_tag="PLU11_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HPN60266.1"
                     /translation="MRKNKIGLDAAKAKVLGDKLNVLLSNLQVLYINSRGFHWNIKGD
                     NFFELHLKFEELYTDLQLKIDEVAERILTLGHVPDHSYTDYLKHAKITEVTNQSNGKD
                     ALKSVMDSISILLEEEREILQLAADANDEGTNALMSDYIRQQEKQVWMFCSYLSH"
     gene            complement(2147..2740)
                     /locus_tag="PLU11_13865"
     CDS             complement(2147..2740)
                     /locus_tag="PLU11_13865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN60267.1"
                     /translation="MQQFWNERYQENEHVYGEAPNAFFKSFIDPLQPGTLLLPAEGEG
                     RNAIYAASRGWVVEAFDYSEVAREKALKRAASKGVSIRYDLQDISLFKASKQYDMVAL
                     LYVHLPEPLRHNFHTAIYKSVKPGGFLLLEAFAKEQQGFDSGGPKDPALLYDAPSICS
                     DFPFLHQLSCEQKEVILDEGPFHQGKAAVLRLAGQRL"
     gene            2848..>3362
                     /locus_tag="PLU11_13870"
     CDS             2848..>3362
                     /locus_tag="PLU11_13870"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN60268.1"
                     /translation="MKIVFLSATLFFLLAACNDNPSVKAQGPDSANKIVETTPAPPTV
                     IPDTMQTNTTPDPAPATASALPSFRMRNGRGEIVDLAQFRGKKVFVNLWATWCPPCRA
                     EIPSIESLGTKTDPGKVEFVMLSLDDNFDKAKTYAANNNMKLPVYYPASDLPQLFVTD
                     GIPMTVIFNEKG"
BASE COUNT          895 a          799 c          744 g          924 t
ORIGIN      
        1 ttatcccatt ctttaaaatc ggtcataaaa ccttcttcgt ttacgtggat ggttttacct
       61 gctattactt tttccataaa ataaattttt aagtgtgaat gattgtatta tacagtttct
      121 gttttttcat aatgcttacc agactctgac atggtagcgg agacaaacgg aatatgcgtt
      181 cctttcagca acatgtgcca gtaaacccag cggaaggcca gtttacccat atggttcatc
      241 cggctttctt taagtaaccg cagcgggcca agtccaggga aggggaaggt tccttcaacc
      301 ggctcatgcg tgtagttgaa gtcgatcagg agggctttac cgccgcctgt ttcaatgaaa
      361 cagttggcat gtccgtcaaa ctcttctttc agtggctccc cttttacaaa gcgtaaaatg
      421 ttttcagtca gaatctcggc ttcaaagtgg gctacagaac cggctttgga agcggggata
      481 ttactggcat cacccagtac aaacacattc tggtaagcct tggattgcaa agtggctttg
      541 ttggtcggca catagttcag gtcatcaccc ataccggagc gttccatcac ggcatctcct
      601 ttattggtgg gtacggtaac caggatatca aaaggaattt cacgtccgcc gtaatcaata
      661 atctttttgt tttcattatc cacctgctcg atcgcgaaat cgctttcaat attgatatgc
      721 ttttcttcca gcaagtgctc cagcgtggcg gtggctttgg gtttggtaaa ggcgccactc
      781 agcggagtta catatgtaat ctccacttta tcccgcatgt gtttattctt gaaaaatgaa
      841 tcggccagga aggcaaattc caacggggca accgggcatt tgattggcat ttcggtgata
      901 tgtaccacca gtttaccgcc tttccagtct cttaacttat tccggagatt caacgccccc
      961 tcaaaagtat aaaaatcaaa aacggactga tgccattcac ttcccatcat tccttctgtt
     1021 tcctccgggg caattttggc accggtggcc acgatcatta cgtcgtaaac aaccacactg
     1081 ccgtctgtca tcatcacttt attttcggca ggcacaatcc ggtcaataga agcctggatc
     1141 agatcaacgg ttttaggaat gaactcctgg atggatttca caatatcttc cggctcataa
     1201 atatcaaagg gaagaaagag gtatcctggc tgatagtggt gttcagtacg ttcatcgata
     1261 atactgatac gccattcggg tcttttgaga tgatggctaa ggtgattggc catcatggta
     1321 ccggccgtac ctgctccgag aatgacaagg tgtttcatgt tgagcgtttt ttgtgagtgt
     1381 tgaattacga tgtaaaattg cacccgccaa ctccccggtt ctgcgtgttc tgtcacagtt
     1441 ccggatgatt cgcctcaggc ttccagttga tttatcaacg gataaccctg attttccctg
     1501 tttcagtgat tgaagtcaca tttcgttaag tcatgctttt gtaaaaaacc ggtcagaaaa
     1561 gacaaccttg tctgaaatga aaatcagggc ttttggaatt tttaactgag gttaatcaaa
     1621 tacccgcaaa cggccattgg ttaactttgt actgaaaatg atgaatcatg agaaaaaaca
     1681 aaatcggcct tgatgccgct aaagcgaaag tgctgggaga caaactgaat gtattattat
     1741 ccaacctgca ggtgctgtat atcaacagcc gcggttttca ctggaatatc aaaggtgata
     1801 atttctttga attgcacctg aagtttgaag aactctatac ggatctgcaa ctgaagatcg
     1861 atgaagtggc tgaacggata ctcaccctgg gccatgtacc cgaccacagt tatacggatt
     1921 acctgaaaca tgcaaaaatt acggaagtta ccaaccagtc aaatggaaag gatgctttaa
     1981 agtcagtaat ggacagcatc agtattctgc tggaagaaga acgggaaatc ttacagttag
     2041 ccgcggatgc caatgatgaa ggcaccaacg ccctgatgag tgattatatc cgccagcagg
     2101 aaaaacaggt atggatgttc tgctcatacc tgagccatta accgcattat aaacgctgac
     2161 cggccagccg taatacggct gctttgccct ggtgaaacgg cccttcatcg aggatgactt
     2221 ctttttgttc gcaggaaagc tgatgcagaa aaggaaaatc actgcaaatg gaaggcgcat
     2281 catacaatag tgcagggtct ttcggaccgc cactgtcaaa tccctgttgt tctttggcaa
     2341 aagcttccag caacaggaat ccgcccggtt ttacagattt atagatggcc gtatgaaagt
     2401 tatgccggag aggttcgggc aggtgtacat acagcaaggc caccatatca tattgtttgg
     2461 atgctttaaa aagagaaata tcctgcaggt cgtagcggat actaacccct ttagatgctg
     2521 ccctcttcag cgctttttcc cgggctacct cactataatc aaatgcttcc accacccagc
     2581 ccctggaagc ggcgtagatc gcgttacgcc cctctccctc tgccggcaac aacagcgtgc
     2641 ctggctgaag ggggtcaata aaggacttga aaaacgcatt gggcgcttcg ccgtatacat
     2701 gctcgttttc ctggtaccgt tcgttccaaa actgctgcat ttgaattagt tttaacaaat
     2761 ttgcatctta gaccctgatt cagcggtgat attaatcacc gatctttcca ctgaatcacc
     2821 ggattttgca cctcaaatta ttaaaatatg aaaattgttt tcctttccgc taccctgttt
     2881 tttttactgg ccgcctgcaa tgataaccca tccgttaaag cccagggtcc cgattccgcc
     2941 aataagatcg ttgaaacaac cccggctccc ccgacggtta ttccggatac aatgcagaca
     3001 aataccacac ccgatcctgc accggcaacg gcctcggccc tcccgagttt ccgtatgcgg
     3061 aatggccgcg gagaaattgt ggacctggcc caattcaggg gcaaaaaagt atttgtgaat
     3121 ttatgggcta cctggtgccc gccctgccgt gctgaaatac cttccattga aagtcttggt
     3181 accaaaacgg accccggcaa agtggaattt gtcatgctct cgctggacga caattttgac
     3241 aaagccaaaa cctatgcggc taataataat atgaagcttc ctgtttatta cccggcgagc
     3301 gacctgcccc agctttttgt aacggatggg attccgatga cagttatttt caatgaaaaa
     3361 gg
//
LOCUS       DAOPMW010000380         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33935_length_3361_cov_6.846038, whole genome shotgun sequence.
ACCESSION   DAOPMW010000380 DAOPMW010000000
VERSION     DAOPMW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33935_length_3361_cov_6.846038"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLU11_13875"
     CDS             complement(<1..607)
                     /locus_tag="PLU11_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN60269.1"
                     /translation="MLLVVKRTCLLLSGLFFALLLQAQNSEVPKGWHMMDKKASGYYG
                     ISSEKAYQFVKSKKLKSNTVIVAVIDSGIDTLHEDLKSVLWTNPKEIPGNGIDDDKNG
                     YVDDVHGWNFLGGRDGQNVDKDSYEAARVYHRYKSKFADKNIKPEELSAEEAIEFADW
                     KRAEKEVVGENKQNGLELLMMRRAYNNCLKADTVLRKALGKD"
     gene            complement(727..2142)
                     /locus_tag="PLU11_13880"
     CDS             complement(727..2142)
                     /locus_tag="PLU11_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN60270.1"
                     /translation="MRKIYAFLFILLGCGLISAAQVRVAIVAGGHQSTVIEENELPNW
                     NDIKNNYTGRIGFHTGFIADIAFSPKSKLFFQPGVIYYNKGRKYAQSFDPPVGTIVRQ
                     QSTQYINYIDLPLNLVLKFGQRTKFVIGGGPYGSFFYTGKETSQTELNTGVVDSEENS
                     DLSVGNQPGQYRTYNFGVNGLVGVEFKGFFITANYSRGLNDFYESTSYIGSFRHQTIG
                     GTIGVFLGKPEKIEKKVLDKDKDGIEDVKDGCPDEPGTAATNGCPDKDADGIADKTDK
                     CPDMAGLIKYNGCPVPDTDKDGINDENDKCPNEFGVIRYAGCPIPDKDGDGVNDEEDK
                     CPEVKGTRQYNGCLPEEVKKEIIEKVNYAAKRLQFKQADAVLLPNSLKVLDEVAKILQ
                     DNPDIKLSIEGHTANNGYFDANMRLSQQRADNVKAYLEKKGISSERLKAKGFGPTQPV
                     SKGKSPAEVALNRRVELKLSY"
     gene            complement(2249..>3361)
                     /locus_tag="PLU11_13885"
     CDS             complement(2249..>3361)
                     /locus_tag="PLU11_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN60271.1"
                     /translation="PAHRMYEQYHPLGIVGIISAFNFPVAVWSWNTMLAWVCGDVCVW
                     KPSEKVPLCAVACQNIIADVFRRNNIPEGVSGLVIGGREIGEWMSADTRVPLVSATGS
                     TRMGKAVGTTVAARLGRSLLELGGNNAIIISKEADLNIAILGALFGAVGTAGQRCTTT
                     RRLIIHEDVYESVKNKLVAAYQQLKIGDPLDQHNHVGPLIDKDAVRMYLASIDACKKE
                     GGRFIVEGAVLEGPGYESGCYVKPCIAEAEPGYQIVQHETFAPILYLLKYKTMEEAIA
                     IQNEVPQGLSSSIMTLNMREAEQFLSHAGSDCGIANVNIGTSGAEIGGAFGGEKETGG
                     GRESGSDAWKAYMRRQTNTINYSQSLPLAQGIRFDL"
BASE COUNT          840 a          856 c          701 g          964 t
ORIGIN      
        1 catctttacc cagggccttg cgcaaaacag tatcggcttt caggcagtta ttgtacgccc
       61 tgcgcatcat cagcagttca agaccattct gtttgttttc gccaaccact tctttttctg
      121 cacgtttcca atcagcaaat tcaatcgctt cttctgcaga caactcctca ggttttatat
      181 ttttatccgc gaactttgat ttataccggt gataaacacg ggctgcttca taggagtcct
      241 tgtcaacatt ttgtccatcc cggcctccca ggaaattcca accatgtaca tcgtctacat
      301 aaccgttttt atcatcgtca attccgttcc ccgggatttc tttggggttg gtccacaaaa
      361 ctgatttaag gtcttcgtgc agggtatcga taccggaatc aataacggcc acaatcaccg
      421 tattgctttt taattttttt gatttgacaa actggtaggc tttttcagaa ctgattccat
      481 aataaccact ggcttttttg tccatcatat gccagccttt aggcacttca ctgttctgcg
      541 cctggagcaa cagggcgaag aataaaccgg ataggagcag gcaggtacgt tttactacta
      601 ataacattga tcagatttta ttttacaaat ttggtatagg cgtaaaaata atgattacca
      661 ttcatacaat tttcacaaat aaagccccgg aaaaccgggg ctttagcatt ttattcacgt
      721 tggggatcag taactcagtt tcagttccac cctccggttc agcgcaacct cagccgggga
      781 tttacccttg ctgaccggct gtgtaggtcc gaaaccttta gctttcagcc tttcagaact
      841 gattcctttc ttctccaggt aggcttttac attgtctgcc cgctgctgag aaaggcgcat
      901 attggcatca aaatacccgt tattggcggt atgtccttcg atggataact taatatcggg
      961 gttatcctga aggattttag caacctcatc cagcactttc agggagttgg ggagaaggac
     1021 ggcgtctgcc tgtttgaact ggagccgttt ggcggcataa tttactttct caataatctc
     1081 ctttttcact tcttccggga ggcaaccatt atactgacgg gttcctttta cttccggaca
     1141 tttatcttct tcatcattta ctccgtcccc gtctttatcg ggtatcgggc aacctgcata
     1201 acggatcacg ccaaattcgt tcggacattt atcattctca tcattaattc cgtccttatc
     1261 ggtatcaggc acggggcaac cattatattt aatgagaccg gccatatccg ggcatttgtc
     1321 tgttttatcg gcaatcccat ctgcgtcttt atccgggcaa ccattggtgg cagcagtacc
     1381 gggttcatcc ggacaaccat cttttacatc ttcaattccg tctttgtctt tgtccagcac
     1441 tttcttttca attttctccg gttttcccag gaaaacgcca atcgttcctc caatagtctg
     1501 atgacgaaaa ctaccaatat aggaagtaga ctcatagaaa tcattcaatc cgcggctgta
     1561 attggctgtg ataaaaaaac ctttaaactc cacgcccact aaaccattca cgccaaaatt
     1621 atacgtacgg tattgccctg gctggtttcc taccgacaaa tcactgtttt cttccgagtc
     1681 aaccacaccg gtattcagtt cagtctggga ggtttcctta ccggtataga agaaggaacc
     1741 atatgggccg ccaccgatta cgaatttggt tcgctgacca aatttcagca ccaggttcag
     1801 gggaagatca atataattga tatactgggt gctttgctga cgtacaatag tcccaaccgg
     1861 cgggtcaaag gactgggcat acttacgtcc tttattataa taaataaccc cgggctggaa
     1921 aaagagtttg gatttggggg agaacgctat atcagcaata aaaccggtat gaaaaccgat
     1981 ccggccggta tagttatttt taatatcatt ccagttggga agttcatttt cctcgataac
     2041 cgtggactgg tgtcctccgg caacgattgc cactcttacc tgggcggccg aaatcagccc
     2101 gcaaccaagc aggatgaaga ggaaagcata aatctttctc ataggcgttg attgtcgcat
     2161 taatgggttt gtttgcaaaa gtcaacaaaa aaaccggtat atcataatat gccggttttc
     2221 aatgttttca acagctgtgg agatcttatc agaggtcaaa cctgattccc tgtgccaggg
     2281 gcagtgattg gctgtaattt atcgtattcg tctggcgccg catataagct ttccaggcat
     2341 cactgccgct ttcccggcca ccacctgttt ctttttcacc accgaatgct ccgccgattt
     2401 cagccccact ggtgccaata ttgacattgg caataccaca atcactgccg gcatgagata
     2461 gaaattgttc ggcttcccgc atattcagag tcataatgga ggaggagagt ccctggggca
     2521 cttcgttctg tatggctatg gcttcctcca ttgttttgta ctttaataag tacaggatcg
     2581 gggcaaaggt ttcatgctgc acgatctgat aacccggttc tgcttcggca atacagggtt
     2641 ttacataaca accgctttcg tagccgggtc cttccagcac ggcaccttct acaataaacc
     2701 gtcctccttc ttttttgcag gcatcaatgg atgccaggta catccgtaca gcatccttat
     2761 caattaatgg tccgacatga ttatgctgat ccaacggatc accgatcttc aattgctgat
     2821 aagcggctac cagtttattt tttacggatt catacacgtc ctcatgaata atcagccggc
     2881 gggtagtggt acagcgttgc ccggcagttc ccaccgcacc gaataaagca cccaggatgg
     2941 caatattcag atctgcttct ttggaaataa taatagcgtt gttgccaccg agttccagta
     3001 aggaacggcc cagcctggcc gcaaccgtgg tgcccaccgc tttacccatc cgggtagaac
     3061 ccgttgcgga aacaaggggt acgcgggtat cggcagacat ccattctccg atttcccgac
     3121 caccaattac cagtccggaa accccttccg gaatattatt tctccggaag acatccgcta
     3181 ttatattctg acaggcaacc gcgcaaagcg gaactttttc agagggtttc catacacata
     3241 catccccgca aacccaggcc agcatcgtat tccagctcca taccgcaacc gggaaattga
     3301 atgcggaaat gatacccaca atacccagcg gatgatattg ttcatacatc cggtgtgcgg
     3361 g
//
LOCUS       DAOPMW010000381         3360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_33946_length_3360_cov_6.839939, whole genome shotgun sequence.
ACCESSION   DAOPMW010000381 DAOPMW010000000
VERSION     DAOPMW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33946_length_3360_cov_6.839939"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLU11_13890"
     CDS             complement(<1..493)
                     /locus_tag="PLU11_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60272.1"
                     /translation="MTKLLWLLPLTILLYACPFESNMPLEKAPVEPIDSGLLGFWYGI
                     VKDGSDYFGIEALEISRQSDSLYSIIRYGKAVKGDMILPDTSFYTAFSSRVRDQSFMN
                     LAALGPDGSPNRIWYLSAFSRSQDTLQVRTVTETFTTRKSFPRPEDFKKLVLDMSQNR
                     NIYD"
     gene            complement(534..1586)
                     /locus_tag="PLU11_13895"
     CDS             complement(534..1586)
                     /locus_tag="PLU11_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN60273.1"
                     /translation="MKLIFVFAFIVIYFTPSIACYNESHIIKSGKQTNRVEPMALFYN
                     EPDKAEAKSFLAKYDLAKIKTYKKDIQSDIAVNLGYLGRYTEALDILKKLQAAYPGDY
                     NITANLGTTYELLGKNELALQFIKKGMELNPDSHEGSEWVHVKILEAKLKMAGQPGWI
                     YANRVLGTGVNFDSKESEALNDKAWDVEYQLQERIPFTPFPDALLANLFDELGDLYAT
                     QQSVELAYIAYDFALRNDSSDHFGVKAKMEALKPVLKKNKIPVPSWKEHYYNRQLSKI
                     QSEITEEVIEQLTNPEKLKKTTETLNKLVDGFSGRNKEKEAKNRQNNLILAGSIILAG
                     FAGIFIYTRLKKPKTS"
     gene            1671..>3360
                     /locus_tag="PLU11_13900"
     CDS             1671..>3360
                     /locus_tag="PLU11_13900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN60274.1"
                     /translation="MVKPYILFFLLIGHLLVAAQTGPAYPKGYFRNPVGIPLQLSANF
                     GELRPNHWHMGLDVRTQAKENLPVYAAAQGYIAKIGIRSQSFGRFIIINHPNGLSTLY
                     GHLNNFYPELEAWVTAQQYDQESWAVELDIPKEKFPVSKGTFIAYSGNTGGSQGPHVH
                     FEIIDTKTTKRLNPLLFGFPIADNVPPALIKLAMYDRGRSVYDQSPRFFPLKNTDSGY
                     IIPKLPVIETGLSRISFALQAYDRLSGSTNPNGIYSATLYLDEQPQVSFILDSVDYDE
                     TAYMNAHVDFKLRANGGGWLQHLSAMPGDAGSVYKKINGDGVLHLVDTTLHTVRVVVS
                     DAYGNQSSLQFQVKYNDSLSRPIFRQPDAMYFIPQEQTQLSKPDFQLNMSERGLYDTV
                     KAYYSRGTNVIPANALSPVHQVNDADVPVHAPFTVRIKPYRAAPGGTEGKLLLQRTFR
                     SGRTVKKAAWDNEWLKAEFDDFGYFQAFFDTIPPELDELGKGDTINLSAATRIIFSPS
                     DNFGVLRNFRAELNGQWLRFTNDKSRNWIYIFDERCPYGVHELKVSVEDLVGNRT"
BASE COUNT          858 a          749 c          801 g          952 t
ORIGIN      
        1 catcataaat attccggttt tgactcatat caagtaccag ctttttaaaa tcttccgggc
       61 ggggaaagga cttgcgggta gtaaaggttt cggttacagt ccggacctgt aaggtatcct
      121 ggctccggct gaaggcggag aggtaccaga tcctgtttgg acttccatcc ggacccaggg
      181 cggccaggtt cataaaggat tgatccctga cccgggaact gaaagcggta taaaaagagg
      241 tatccggcag gatcatatcg cctttgacag ccttgccata ccggattatg ctgtagagag
      301 aatctgattg acggctgatt tccagagctt caatgccgaa atagtcgctg ccatccttca
      361 caatgccata ccagaaaccg agcaggcctg agtcgattgg ttccaccggg gctttctcca
      421 ggggcatatt cgattcgaaa ggacaggcat acaataaaat ggtcaggggc aggagccaca
      481 gtagtttggt cataacaggc gaaaaagata caaaatgtcc ggtgaaaggg gacttagctt
      541 gtttttggct ttttcaatcg ggtataaata aagataccgg caaatccggc caggataatg
      601 gagccggcaa ggatcaggtt gttttgcctg tttttggctt ccttttcttt attccggccg
      661 ctaaacccat ccaccagctt atttaaagtc tcggtggttt ttttcagttt ttccggatta
      721 gtcagctgct caattacttc ttctgttatt tctgattgaa tcttactgag ttgccggtta
      781 taataatgtt ccttccagga agggaccgga atcttgttct ttttcagtac aggtttcaat
      841 gcttccattt ttgcttttac gccaaaatga tcggacgaat cattccgtaa cgcaaaatca
      901 taagcgatat aggccagttc aaccgattgc tgggtggcat agagatcccc gagttcatca
      961 aataaattag cgagcagggc atccggaaaa ggggtgaagg ggattctttc ctgtaactgg
     1021 tattccacat cccaggcttt atcattcagg gcttcggatt cctttgaatc aaaattcacc
     1081 ccggtaccta ataccctgtt agcatagatc cagcccggtt ggccggccat tttcagtttt
     1141 gcttccagga tctttacatg cacccactca gatccttcat gcgaatccgg gttcagctcc
     1201 attccttttt taatgaattg taatgccagt tcatttttcc ccagtaattc ataagtggta
     1261 ccgagattgg ccgtgatatt gtaatctccg ggatatgccg cctgcagttt ttttaaaatg
     1321 tccagggctt ctgtgtaacg acccaggtag cccagattca cggcaatgtc tgactggata
     1381 tcctttttat aggtttttat tttagccagg tcatatttgg cgaggaagga cttggcttcc
     1441 gctttatccg gttcattata aaaaagcgcc atgggttcta cccgattggt ttgttttccg
     1501 cttttaataa tatgcgattc attgtagcag gcaattgacg gggtaaaata aattacaatg
     1561 aaagcgaata cgaatatcaa tttcataacg gatatggttt attttaaatg attcctggtg
     1621 attttgggag taaatttgtt tttaaattta ataaaaaagt atacagcctg atggttaaac
     1681 cgtatatcct gttttttttg ctgatcggtc atttgttggt agcggcgcaa accgggccgg
     1741 cctatcccaa aggttatttc cgtaatccgg tggggatacc gctgcaactt tctgccaatt
     1801 tcggtgaact ccggcccaat cactggcata tgggtcttga tgttcggaca caggcaaagg
     1861 aaaatctccc ggtatatgcg gcggcacaag gttatatcgc caaaatcggc atccggtccc
     1921 agagtttcgg tcgttttatc attatcaacc atcccaacgg gctctccact ttatacgggc
     1981 atttgaataa tttttatccc gaactggaag cctgggtaac ggcgcaacaa tatgaccagg
     2041 aatcctgggc ggtggaattg gatattccca aggagaaatt tccggtgagt aaaggaacct
     2101 ttatcgccta cagtgggaac accggcggat cacaggggcc gcatgtgcat ttcgaaatca
     2161 ttgataccaa aactaccaaa cggctgaatc cgcttttgtt tggatttcca atagccgaca
     2221 atgttccgcc agccctgatc aaactggcca tgtatgaccg cggccgttcg gtgtatgatc
     2281 agtcgccccg gtttttcccg ttaaaaaata ccgacagcgg gtatatcatc cctaagttgc
     2341 cggttataga aacgggatta tccaggatca gttttgccct gcaggcttat gaccggctgt
     2401 ccggctccac caatcccaat ggaatctatt cggctacctt atatctggat gaacaaccac
     2461 aggtatcttt tatactggac agtgtggatt atgatgaaac ggcttatatg aatgcacatg
     2521 tggacttcaa gctccgggcc aacggtggtg gatggctgca gcatttatca gcaatgccgg
     2581 gtgatgccgg atcggtgtat aaaaaaatca acggggatgg agtgcttcac ctcgtggata
     2641 ccaccctgca taccgtgcgg gtcgtcgtca gtgatgctta tggcaatcaa tcttcgctgc
     2701 aatttcaagt gaaatataat gacagtttgt cccgccccat atttcggcaa ccggatgcta
     2761 tgtactttat accccaggaa caaacgcaat tgagtaaacc cgattttcag ctgaatatgt
     2821 cggagcgagg tttgtacgat accgtaaaag cttattattc ccgtggtacg aatgtcattc
     2881 cggccaatgc attatctcct gtacatcaag tcaacgatgc ggatgtaccg gtgcatgctc
     2941 catttacagt aaggatcaaa ccctatcgtg cagcccctgg gggaacggaa ggaaaattat
     3001 tgctccagcg gacattccgc agtggtcgaa ccgtaaaaaa agctgcctgg gataatgaat
     3061 ggctgaaagc ggagtttgat gacttcggtt atttccaggc cttttttgat actattcctc
     3121 cggagctgga tgaattaggc aagggcgata cgattaacct gagtgcagct acccggatta
     3181 ttttttcacc ttccgataat tttggggtat tacgaaattt ccgcgctgaa ctaaacggtc
     3241 agtggctgcg ttttaccaat gacaagagcc gtaactggat ctatattttt gatgaacgtt
     3301 gtccatacgg ggtgcatgaa ctgaaagtaa gtgtggagga cctggtgggc aaccggacga
//
LOCUS       DAOPMW010000382         3349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_34093_length_3349_cov_7.033090, whole genome shotgun sequence.
ACCESSION   DAOPMW010000382 DAOPMW010000000
VERSION     DAOPMW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34093_length_3349_cov_7.033090"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..954
                     /gene="atpG"
                     /locus_tag="PLU11_13905"
     CDS             73..954
                     /gene="atpG"
                     /locus_tag="PLU11_13905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPN60275.1"
                     /translation="MSGQLKEVRSRIKSVQSTQQITKAMKMVSAAKLRRAQDAIIQMR
                     PYAQKLQEMLSNIVSNSEAGTGMALATERPVEKVLMIVITSDRGLAGAFNANVIKMTK
                     ATIAEKYAEQYKKGNLTVWGIGKKGMEFFIKNNYKTNDSFRDIFLHLNFENVQKAAQA
                     ARTAFINKEFDVVELVYSQFKNAATQKFVVERFLPIPKVEGKAGAAKADFIYDPSKEI
                     LISELMPKILNTQLFKAVLDSNASEHGARMTAMDKASENANEMLRSLKISYNRARQAT
                     ITTELTEIVSGAAALQG"
     gene            1366..2073
                     /gene="scpB"
                     /locus_tag="PLU11_13910"
     CDS             1366..2073
                     /gene="scpB"
                     /locus_tag="PLU11_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPN60276.1"
                     /translation="MTIDNLIPHIEALIFASDKPLTTMEVTELINNAFGFMEEKIMLD
                     QAESAIDGIREKYASEFYPFELRQSGGGWQFLTKKEFHKTIAQLNGDKFLKRLSAASM
                     ETLAIIAYKQPVTKGEIEAIRGVSSDYAVQKLLEKELIFITGRNEKMPGHPLVYCTSK
                     NFMDYFGINSADDLPKIKEVLADQLVEPTIMKDMQVTEEEESTERNDDMDGTTALAVT
                     EGGELVINDDAGESEEN"
     gene            2149..>3349
                     /gene="lysA"
                     /locus_tag="PLU11_13915"
     CDS             2149..>3349
                     /gene="lysA"
                     /locus_tag="PLU11_13915"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPN60277.1"
                     /translation="MATELSHEQLSALAREYGTPLYVYHAERIREQYNNLLEAFRGAD
                     VRFFYAAKALTNINVLRYVNHIGCNVDCSSVNEARLALHAGFDPEKVLYTSNNVSFEE
                     IEEAQSLGVHINIDSISNLRKFGRKFGHSYPVGIRLRPNIMAGGNLKISTGHADSKFG
                     IPLENITEVLDVIRETNLFIRTLHIHTGSEIKDVEVFARGIEVLFEVAQQFPELEVID
                     LGGGFKVPYMPGEEGTDIPLLGKKVKEEFEQYEAKYGRKLQAWFEPGKYIVSECGYFI
                     TKVNVLKPAVNAVFAGVDTGLNHLIRPMFYNAYHQIENISNPTGAIQQYNVVGNICET
                     DTFAENRPLPEIREGDFLVFRNAGAYCFEMSSHYNSRFKPAEVMVEDGTARLIRKRDE
                     LSDLLAGQ"
BASE COUNT          945 a          718 c          819 g          867 t
ORIGIN      
        1 gtttaatttt aaaaagaagc atggattttt accttctgcg ccctaccttt gcggccaatt
       61 taagtatcaa ctatgtcagg tcagttaaaa gaggtacgta gccggattaa atccgttcaa
      121 agcacgcagc agatcaccaa agcgatgaag atggtaagtg cggctaaatt gcgccgtgca
      181 caggatgcga ttatccagat gcgtccttat gcccagaagc tgcaggaaat gctcagcaac
      241 atcgtgagca acagtgaagc cggaacaggg atggccctgg ctaccgaacg ccccgtggag
      301 aaagtcctga tgatcgtgat taccagtgac cgcggtctcg ccggcgcttt caatgccaac
      361 gtgatcaaaa tgaccaaggc gaccattgct gagaaatatg cagagcaata taaaaaaggc
      421 aatctgaccg tatggggtat tggtaaaaaa ggaatggagt tctttattaa gaacaattac
      481 aaaaccaatg actctttccg tgatatattc ctgcacctga attttgagaa tgtacagaaa
      541 gccgcacagg ctgcccgtac tgcttttatc aataaggagt ttgatgtggt ggagctggta
      601 tacagccagt tcaaaaatgc agccacccaa aaatttgtgg tagagcgttt ccttccgatc
      661 cccaaagtgg aaggtaaagc cggagcagcc aaggctgatt tcatttatga tccttccaag
      721 gaaatactga tctccgagct gatgccgaag atcctgaata cacagctgtt caaggcggta
      781 ctggattcca atgcttccga gcacggtgcc cgtatgaccg ctatggacaa ggccagcgaa
      841 aatgccaatg aaatgctacg ttccctgaag atttcctaca acagggcccg ccaggcaacc
      901 attaccaccg agctgactga gattgtgagc ggagcggcgg ctttgcaagg ataaatttaa
      961 gtacgaatta cgaagtacga agtacgaagt acgaaagaat acagaaccgg tttacaagtt
     1021 gtagaccggt tttttatggg gaaagtacga gtcggcagtc ggcagtcggc agtcaacagc
     1081 cccttctaag aagagatttc taaactcttc tttatatctc tttctgcttt agtaaatcat
     1141 aaaccttcta aacccttctt tcagcctctt tctgctttag tacctcataa accttctaaa
     1201 ccctcctttc ggcctctttc tgctttaagc ccccctaaac cctccttatg agtgtgaata
     1261 aactgtgtat agcaagtccc ttgtgatagt tatcttgcca atccgtttgg cgttgatctg
     1321 agtgatacgg agcggttgat tttcacctta tttgactata ctattatgac tatcgataat
     1381 ctcatacccc atattgaagc actgattttt gccagtgata agccgcttac taccatggaa
     1441 gtgacggagc ttattaataa cgcttttggt ttcatggaag agaagattat gctcgaccag
     1501 gcagaatcgg ccattgacgg gattcgcgaa aaatatgcgt ctgaatttta cccctttgaa
     1561 ttaaggcaga gtggtggtgg ctggcagttt ctgaccaaga aggaatttca taaaaccatt
     1621 gctcagttga atggggataa attcctgaaa cgcctttccg ccgcatctat ggaaaccctg
     1681 gccattattg cctataagca acccgtgacc aagggagaaa tcgaggccat ccgcggggtg
     1741 agttcagatt atgcggtgca gaagctgctg gaaaaagaac tgatctttat taccggccgc
     1801 aatgaaaaaa tgcccggcca tccgctggtg tattgtactt ccaagaactt catggactat
     1861 ttcggaatca actccgccga tgatttacca aaaatcaaag aagtgctggc cgaccagttg
     1921 gtggagccaa ccattatgaa agatatgcag gttactgaag aagaggagtc aaccgaaaga
     1981 aatgatgaca tggatggtac aaccgcactt gccgttaccg aaggcgggga actggtgatc
     2041 aatgatgatg ccggtgaatc agaagaaaat taagtattac ttattatccc aaatttataa
     2101 accggctcag ttcattactg agccggttgt ttttttatct tcgttcctat ggcgactgaa
     2161 ctttcacacg aacaactgtc agcattggcc cgtgaatacg gtaccccttt atatgtatac
     2221 catgcggaaa gaatcaggga acaatacaat aacctgctgg aggctttccg cggggcggat
     2281 gtacgttttt tttatgcagc caaggcgctg acgaatataa atgtcctgcg ttatgtcaac
     2341 catatcggat gtaatgttga ttgcagttcg gtgaatgaag cgcggctggc gctccatgcc
     2401 ggttttgatc cggagaaggt gctgtatact tccaacaatg tgtcttttga agaaatagaa
     2461 gaagcccaaa gcctgggggt gcatatcaat attgacagta tttccaatct ccggaagttt
     2521 ggccgaaaat tcggtcatag ctacccggta ggcatccggc tcaggcccaa tatcatggcc
     2581 ggtggtaacc tgaagatatc taccggtcat gcggacagca agtttggcat accattggaa
     2641 aatataaccg aggtactaga tgtgatccgg gaaaccaacc tgtttatccg taccctgcat
     2701 atccataccg gtagtgagat taaagatgtg gaggtatttg ccaggggtat agaagtattg
     2761 tttgaagtgg ctcagcaatt tccggaactc gaagtgatcg atctcggggg tggatttaaa
     2821 gtaccgtata tgccgggcga agaaggaacc gatattccgt tgttggggaa gaaagttaag
     2881 gaggagtttg agcagtacga agccaaatac ggacgcaaac tgcaggcctg gtttgagccg
     2941 ggtaaatata tcgtgagtga atgtggttat tttattacga aagtcaatgt gctgaaaccg
     3001 gcggtcaatg ccgtatttgc cggggtggat accggattaa atcacctgat ccgcccaatg
     3061 ttttacaacg cttatcacca gattgaaaat atctctaatc ccaccggtgc tatacagcag
     3121 tataatgtgg tgggtaatat ctgcgaaacg gacacttttg ctgaaaaccg gccgcttccc
     3181 gaaatccgcg aaggggattt tcttgttttt cgcaatgccg gggcctattg ttttgaaatg
     3241 tcatcgcatt ataattcccg gtttaaacct gcggaagtaa tggtagagga tgggacggcc
     3301 aggcttatac gaaaaaggga tgagttgagt gatttgctgg caggacagg
//
LOCUS       DAOPMW010000383         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_34141_length_3346_cov_4.855059, whole genome shotgun sequence.
ACCESSION   DAOPMW010000383 DAOPMW010000000
VERSION     DAOPMW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34141_length_3346_cov_4.855059"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1872
                     /locus_tag="PLU11_13920"
     CDS             193..1872
                     /locus_tag="PLU11_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPN60278.1"
                     /translation="MKKVIAPLLILLTVLYACTYSGKNSLLAKPGDMPADEYTIDTER
                     DTILVTKNGALLKIPQGAIDAGNTKQVTLEIKEAYTIAQMIKNGLTTSSGEEPMSSGG
                     MIYINPKKGQEATIKKAIDVALPTLYKDPEMKLFKGEEGSDGRVNWVKPDTLPVTALD
                     KSLATGKAIFNAKCVACHKLGQRATGPDLAHYLRQYSGDTLLVRGYSLHKPNFFWRNE
                     DGADTLYFNPLSAKLAHINMELWSNQYTYFCNQVNQYGSKGTNFPELREDDYDAIYRY
                     VQNESDLLQLPDPGLSALNACIDSCEIFTKRYNELRNRQEELENSRQKQIEENGLQTE
                     EIRKPGPIAPLDTVIQGITPVFDDKEVVNPANPEAIYYQFSIETFGWNNVDVLLKEVI
                     GNTKADLSVRITGSYQEKLDVFLIVPDKKIYTKGGLKNDGSGRYVFAYTDGSIYLPMG
                     AAGYILALTENADGIAYGLLPFSIQASQELSIELQKSSTESFNKAVNDLNMNDIRVSA
                     RQSKHAESIRKADTNLRDINLELKKTELLRPKNCDCDCGSEGDIGFRELMK"
     gene            2033..>3346
                     /locus_tag="PLU11_13925"
     CDS             2033..>3346
                     /locus_tag="PLU11_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPN60279.1"
                     /translation="MDKLIYLVPVMGLIGLLYTLIKFNWVSKQDAGNDRMKEIANYIA
                     VGAMAFLKAEWKILGYFVVIVGILLAIMAQANPHSHWLISVAFVVGAVFSATAGYIGM
                     KVATRANVRTAQAARTSLSKALNVSFTGGAVMGLGVSGLAVLGLGGLYIVLKQYFAPG
                     ASVDSEEMIRTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATIVLGQQTTIDFGNDTLGGYSPI
                     VLPMLIAGVGIIFSMVGTLFVRVSDSAGISTDNVQKALNMGNWGSIVLTAGAAAGLVY
                     FVLPEGTMTLRGFTFTKWGVLGAIGVGLTVGALMSIITEYYTAMGKRPVNSIIRQSST
                     GHATNVIGGLAVGMESTFLPILVLAAGIWGSYEFAGLYGVAIAAAG"
BASE COUNT          894 a          750 c          812 g          890 t
ORIGIN      
        1 ccaattgcaa tttctctcac cccgttaggg gtgcattgtt ggtagcatat caatatgtaa
       61 ccccaaccca atgtttcacc ccgttagggg tgtattgtga tgaccggata aattgaatta
      121 tccaccccaa ttccgggtta ccatttatgc aatttggaat aaatggtatc ttattcttcc
      181 aagaccttat ttatgaaaaa agtcattgcc ccgctcctga tcctcctcac cgtgctttat
      241 gcctgtacct attccggcaa aaattccctt cttgcaaagc ccggtgacat gccagccgat
      301 gaatatacga tcgatacaga acgggatacg atcctggtta caaagaatgg tgctttattg
      361 aaaattccgc agggggccat tgatgcgggg aatacgaaac aggtaaccct ggagatcaaa
      421 gaagcctata ccatcgcgca gatgattaaa aacggactga ccactagttc gggtgaagaa
      481 cccatgagca gcggggggat gatttatatc aacccgaaga aaggacagga ggcaaccata
      541 aaaaaagcga ttgatgtggc cctgcccacc ctgtataagg atccggagat gaaattattt
      601 aaaggagaag aaggcagtga tggccgggtc aactgggtga agcctgatac attgccggta
      661 actgccctgg ataaaagttt agcaaccggt aaagcaattt tcaatgccaa atgtgtcgcc
      721 tgtcataaat taggtcagcg tgccacaggt cctgacctgg cgcattatct ccggcagtat
      781 tcgggggaca cattattagt caggggatat agtttacata agcccaattt tttctggaga
      841 aatgaagacg gcgctgatac tttgtatttt aaccctcttt cagcaaaact ggcccatatc
      901 aatatggagt tatggagtaa ccaatatact tatttctgta atcaggtaaa ccagtatggc
      961 agtaaaggga ccaatttccc tgaattaaga gaagatgatt atgatgcaat ttacaggtat
     1021 gtccagaatg aaagtgacct tcttcaacta cctgacccgg gattaagcgc tttaaatgcc
     1081 tgtattgaca gttgtgagat cttcactaaa cgctataatg aactcaggaa ccgtcaggag
     1141 gaattagaga attcgcggca aaagcagatt gaggaaaacg gattgcaaac agaagaaatc
     1201 cgtaaacctg gtccgatagc ccctcttgat acggtaatcc agggaatcac tcctgttttt
     1261 gatgacaagg aagtcgtgaa tccagcaaac ccggaagcca tttattacca attctccatc
     1321 gaaacctttg gctggaacaa tgtggatgtg ctgctgaaag aggtaatcgg aaatacgaaa
     1381 gccgacctct ccgtccggat taccggaagc taccaggaaa agctggatgt cttcctgatc
     1441 gttccggata aaaagattta taccaaaggg ggattaaaga atgacggttc cggccgttac
     1501 gtatttgctt acaccgatgg cagtatttac ttacccatgg gggctgccgg ctatatttta
     1561 gcccttaccg aaaatgccga tgggattgct tatggattgc tgcccttttc catccaggcc
     1621 agccaggaat tgagcattga actgcaaaaa tcatccaccg aaagtttcaa caaggctgtc
     1681 aatgacttga atatgaacga cataagagta agtgcccgtc aatctaagca tgctgaatct
     1741 atccgtaaag cggatacaaa tctccgggat attaacctgg agcttaaaaa aacggaattg
     1801 ttacgcccca aaaactgtga ctgcgattgt ggatcagaag gcgatatagg atttcgtgaa
     1861 ttaatgaaat agtcccggaa ataagaagtt aaaataagtg tgttttttac acttcaagcc
     1921 cctgaaaata aagcctatca ggggcttgtg ccattgggtc ccaaccccta tctttgccgc
     1981 ccggccaaaa tggccgtttt taatctaaac catacactaa gaatccactg ttatggacaa
     2041 attgatctac ctggtacccg tcatgggact gattggcttg ctttataccc tgatcaagtt
     2101 caattgggta tccaaacaag acgccggtaa cgaccgtatg aaagaaatcg ccaactacat
     2161 cgctgttggt gccatggcct tcctcaaggc ggaatggaaa attcttggct atttcgtggt
     2221 aatcgtgggt atcctgctgg ccattatggc ccaggccaat ccacattctc actggctgat
     2281 ttctgtggcc tttgtagtcg gtgcggtatt tagtgccact gccggttata tcggtatgaa
     2341 agtagccacc cgtgccaacg tgcgcactgc acaagctgca cgtaccagtt taagtaaagc
     2401 actcaatgtc tcctttaccg gcggtgccgt tatgggtctg ggtgtttccg gcctggctgt
     2461 actcggtctg ggcggtttat atatcgtatt aaaacaatac ttcgctcccg gtgcttctgt
     2521 ggattcagaa gaaatgatcc gtacgattga ggtactgacc ggtttctctc tcggtgcgga
     2581 gtcgattgcc cttttcgccc gtgtgggtgg gggtatctat accaaggccg ctgacgtagg
     2641 ggctgacctc gtgggtaaag tagaagccgg tatccctgaa gatgatccac gtaacccggc
     2701 caccattgcc gataacgtgg gagataacgt aggtgacgta gcgggtatgg gcgccgactt
     2761 attcggttct tatgtagcaa cggtactggc gaccatcgta ctcggtcaac aaaccactat
     2821 tgactttggc aatgataccc tgggtggtta ctctcccatc gtattaccaa tgctgattgc
     2881 cggtgtgggt attatctttt ctatggtggg taccctgttt gttcgtgtaa gcgactctgc
     2941 aggtatcagt actgataatg tacagaaagc cctgaatatg ggtaactggg gatccattgt
     3001 gctgactgcg ggtgcagcag ccggactggt ttactttgta ttacccgaag gcacgatgac
     3061 tttaagaggt ttcactttca ctaagtgggg tgtacttggt gccattggtg tgggattaac
     3121 cgtaggtgct ttaatgagta tcatcacgga atattatacc gctatgggca aacgcccggt
     3181 gaactcgatc atccgtcagt cttctaccgg acatgccacc aacgtaatcg gtggtctggc
     3241 cgtgggtatg gaatccactt tccttcctat tctggtatta gccgccggta tatggggttc
     3301 ttatgaattt gccggtcttt acggagtagc cattgccgct gccggt
//
LOCUS       DAOPMW010000384         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_34222_length_3340_cov_12.219482, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000384 DAOPMW010000000
VERSION     DAOPMW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34222_length_3340_cov_12.219482"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1848
                     /locus_tag="PLU11_13930"
     CDS             64..1848
                     /locus_tag="PLU11_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPN60280.1"
                     /translation="MTEPKRLFDCLDYHLERTPLDIMLAGKEGGQWKKHSTASVKETV
                     DRLSAGLLSLGVSCGDMSPEGRDKIAILAKNRPEWVMLDLAVQQIGAVLTPIYPTINV
                     NELEFVLNDAQVKYVFVNDEELFLKVYSLKEKVHSVKEIYTFEHVPNALHWKEVLNLA
                     TPELIAQIKPVSDKITYEDLATIIYTSGTTGTPKGVMLSHRNILSNVNASMPCFPPGE
                     EMKALSFLPLNHIFERMVTYLYLFRGISIYYAESLETIADNLKEVQPHMFTTVPRLLE
                     KVYNKIMQKGNELTGTKRKLFFWAHGLAEKYEINKDQGPMYGFKLGLANKIIFSKWRE
                     GLGGNIKCIVTGGAACQVRLIRIFSAAKITIMEGYGLTETSPVVAVNRYEESGRRFGS
                     VGPLIGGVEVKIAEDGEILCKGPNIMMGYYKRPDLTAEVIDQDGWFYSGDIGDLSADN
                     FLKITDRKKELFKTSGGKYVAPLPIENKLKESPFIEQVMLVGSDRKFVSALIVPSFPN
                     LLDWCRHHGIHATGHEEVIRDPKVQEMYKDLVESFNKYFNHVEQVKKFELLPNEWSVE
                     TGEVTPKLSLKRKVVIEKYKDAIERIYS"
     gene            complement(1851..2321)
                     /locus_tag="PLU11_13935"
     CDS             complement(1851..2321)
                     /locus_tag="PLU11_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60281.1"
                     /translation="MKSSNKILTLVIGLLLVSNIVLVYFMVNGSAKKPPKKDRMDPAE
                     MMIKEVGMDEQQAATHKQMKDDHFKAVRPLYDSLRAAKAVYFGLVKDASVTDSIGKVY
                     REKIAVWQSTIDSMTFAHFRKMRNLLNPQQQVKYDEFVQKLMQRGRKDSAAKSK"
     gene            complement(2321..2662)
                     /locus_tag="PLU11_13940"
     CDS             complement(2321..2662)
                     /locus_tag="PLU11_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60282.1"
                     /translation="MNTNKENRIDEILNSLDGCTQATAPDFFYTRLKARMEKEVAPAA
                     RTSWMLRPAYAFAGMAIILLINVLAVFNQPGTEDNTATSEVDTFQSLASEYRLNEGNS
                     SISMYNLNQDK"
     gene            complement(2713..3276)
                     /locus_tag="PLU11_13945"
     CDS             complement(2713..3276)
                     /locus_tag="PLU11_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN60283.1"
                     /translation="MTEFELIQGLRNGDESAFRYLVDTYQNRVFNTAMGIVQNAEDAE
                     DVAQEVFIQVFRSIGSFKAESKLSTWIYRITTTRSLDLLRSRKSKKRFGFIQRLLGED
                     NEPAQEIPDFNHPGVELDRKENAARLFKVIGKLPENQKIAFTLHKLEDLSYQEVSEVM
                     QTTVAAVESLMHRAKQNLRKLLEKELE"
BASE COUNT          882 a          736 c          820 g          902 t
ORIGIN      
        1 caaaaaaata gtatattgaa cccacgttac acctcaagtg tttacataaa aacaacgatt
       61 gccatgaccg agccaaaaag actatttgac tgcctggact atcacctgga acgcaccccg
      121 ctggacatca tgctggccgg caaagaaggg gggcagtgga aaaaacacag tactgcttcc
      181 gtaaaggaaa cggtggaccg gctcagtgcc ggtttattga gcctgggtgt cagctgcggg
      241 gatatgagtc cggaaggccg ggataagatt gccattcttg ctaaaaaccg acccgaatgg
      301 gtaatgctcg atctggctgt tcagcagatc ggtgccgtgc tgaccccgat ctaccccacg
      361 atcaatgtca atgaactgga atttgtgctc aatgatgcac aagtaaaata tgtattcgtc
      421 aatgatgagg aactcttcct gaaagtatat agcctgaagg aaaaagtgca tagtgtgaaa
      481 gagatctata cgtttgaaca tgtgcccaat gcattgcact ggaaagaagt actgaatctg
      541 gccaccccgg aattgattgc acaaatcaaa ccggtttcgg ataaaatcac ctacgaggac
      601 ctggccacga ttatttatac ctccggcaca acaggtacgc ccaagggggt aatgctttca
      661 caccggaata ttctttccaa tgtgaatgcc agtatgccct gcttcccccc gggagaagag
      721 atgaaggcac tgagcttctt acctctcaat catatatttg agcggatggt cacttatctc
      781 tacctgttca ggggtatttc gatttattat gcggaaagtc tcgagaccat tgccgacaac
      841 ctgaaagaag tgcaaccgca tatgttcacc accgtgccgc gcctgctgga aaaagtgtat
      901 aacaagatca tgcagaaagg caatgaactc acgggcacca aacgtaaatt gtttttctgg
      961 gcgcatggac tggcggagaa atatgaaatc aataaagacc agggaccgat gtatggcttc
     1021 aagctcggcc tggccaataa aatcatcttc agtaaatggc gggaaggact aggtggtaat
     1081 atcaagtgta ttgtaaccgg tggggcagcc tgccaggtgc ggctgatccg catattcagt
     1141 gcggcaaaga tcaccattat ggaagggtat ggattaacgg aaacctcccc tgtggtggct
     1201 gtaaatcgtt atgaagaaag cggccgtcgt tttggttcag taggtccgct gatcggaggt
     1261 gtggaagtaa agattgcgga ggatggggaa atactctgta agggaccgaa tatcatgatg
     1321 ggttattaca aacggccgga tctgacagcg gaagtaatag atcaggacgg ttggttttat
     1381 tccggtgata tcggcgacct gagtgcggat aattttttaa agatcaccga tcgcaaaaaa
     1441 gaattattca agaccagcgg gggaaagtat gtcgctcccc tgcccattga aaacaagctg
     1501 aaggaatctc cttttattga gcaggtgatg ctggtcggtt cggaccggaa atttgtcagt
     1561 gcattgatcg taccctcatt ccctaatttg ctcgactggt gccgtcatca tggtatacat
     1621 gcaaccgggc atgaagaagt gatccgtgat ccgaaggtgc aggagatgta taaagacctg
     1681 gtggaaagtt ttaataaata tttcaatcat gtggagcagg tgaaaaaatt tgaactgctg
     1741 cccaatgaat ggagtgtgga gacgggtgaa gtgacgccta agttatccct gaaacggaaa
     1801 gtggtgatag agaaatataa agatgcaatt gaacggatct atagctgatc ctatttgctt
     1861 ttggcggcac tgtctttccg gcctctttgc atcagttttt gtacaaattc atcgtatttc
     1921 acctgctgct gcggattgag caggtttctc attttccgga aatgggcaaa tgtcatacta
     1981 tcgatagtgc tttgccagac ggcgattttt tcccggtata ctttccctat gctatctgtt
     2041 acagaagcat cttttaccag tccgaaataa acagcttttg ccgcacggag tgaatcataa
     2101 agcgggcgaa ccgctttaaa atgatcatcc ttcatttgct tgtgggtagc ggcctgttgt
     2161 tcatccatac ccacttcttt gatcatcatt tcagccggat ccatccgatc ttttttcggt
     2221 ggctttttag cactaccatt gaccataaag taaaccagta cgatattcga tacgagtaac
     2281 agcccgatca ccagggtcag tattttgttg ctgctcttca tcacttatcc tgatttaggt
     2341 tgtacatgga aatggagctg ttaccttcat tgagccggta ttctgaagcg agggactgga
     2401 aggtatccac ttccgacgtc gctgtattgt cttcggttcc gggttggttg aatacggcca
     2461 gtacattaat cagcaggata atggccatgc cggcaaatgc ataggcagga cgcagcatcc
     2521 aggaagtcct ggcagccgga gccacctctt tttccatcct ggctttcagc cgggtataga
     2581 agaaatcggg ggctgtagcc tgggtacagc catcgaggct gttcaggatt tcatcgattc
     2641 tattttcctt gtttgtattc atataacaag tttttgacgt taacagggga taaaaccttc
     2701 gaattgactt ttttattcca gttctttctc cagtaatttc cggaggtttt gtttggcccg
     2761 gtgcatcagt gactccacgg ctgccacggt ggtttgcatc acctcgctaa cttcctgata
     2821 actaagatct tccagtttat gtaaggtaaa tgcaattttc tgattttccg gcaattttcc
     2881 aatgacttta aacaagcggg cggcattttc tttccggtcc agttccaccc cgggatgatt
     2941 aaaatcaggt atttcctggg ccggttcgtt gtcttcaccc aataatcgtt gaataaaccc
     3001 gaaccgtttt ttgcttttcc ggctccggag caggtccagg gatcgggtag tagtgatccg
     3061 gtagatccag gtcgatagtt tggattcggc tttaaaggaa ccgatagaac gaaaaacctg
     3121 gataaacact tcctgggcca catcttcggc gtcttcggcg ttttgaacga tacccatagc
     3181 ggtattgaac acccggttct ggtaggtatc caccaggtac cggaaggcgg attcatctcc
     3241 gttccgaagc ccttgtatca gttcaaactc agtcaactag cgatcagcaa ttttatgcac
     3301 gaacttacga aagaggcgga taattctttt gtatatttgc
//
LOCUS       DAOPMW010000385         3304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_34802_length_3304_cov_5.734380, whole genome shotgun sequence.
ACCESSION   DAOPMW010000385 DAOPMW010000000
VERSION     DAOPMW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34802_length_3304_cov_5.734380"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLU11_13950"
     CDS             complement(<1..858)
                     /locus_tag="PLU11_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPN60284.1"
                     /translation="MSAAANPVSRIFRLVKLERKEISAIYFYAILNGLIQLSLPVGVQ
                     AIIGFVLGGSMSTSLVILIGLVVAGVFFTGLLQVGQMQLIEKIQQNIFVRYSFELADR
                     IPRLDLKKTDAYYIPELVNRFFEIPALQKGLAKLLLDIPTATIQIVFGLILLSFYHPA
                     FILFGMILVLILWLILYYTGNKGLQTSLTESAYKYGVAAWLEEMGRLIKSFKFSKGSV
                     INLRKTDEKVSGYLDARNSHFNILQFQFKVLIAFKVLITAAMLIIGSLLLVNQQLNIG
                     QFIAAEIVII"
     gene            complement(928..1215)
                     /locus_tag="PLU11_13955"
     CDS             complement(928..1215)
                     /locus_tag="PLU11_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60285.1"
                     /translation="MKKIALFILLLFAIVQVIPGLQAMFSNQVCSVIFNVDEEKNGEK
                     TVDGNQKFNKEYSDFCQLSAILSQRLTIAFHLTEKILPAPSLEQLTPPPNC"
     gene            complement(1319..3223)
                     /locus_tag="PLU11_13960"
     CDS             complement(1319..3223)
                     /locus_tag="PLU11_13960"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPN60286.1"
                     /translation="MEKYNAIYRQSIDNKEVFWKEQAAQLHWFKAPETILSQNEKGFY
                     RWFADGEINTCYLCLDQQVEQGRGEQIALIYDSPVTHTIKKYSYQELLDLVSRFAGGL
                     QSAGIRKGDTVVIYMPMIPQAVIAMLACARIGAIHSVVFGGFAPHELAMRIDDALPKA
                     IISASYGVEFDKTIPYKPLVDRAIEEALHKPPVHIFYERTPGYDELDGISEMDYNQLL
                     LSEPVPCVPVFSGDPLYILYTSGTTGKPKGIIRDHGGYATALKFSMDHIYDAKPGEVY
                     WAASDVGWVVGHSYIVYGPLLQGCTTILYEGKPIRTPDAAAFWRVISEHGVNVMFTAP
                     TAIRAIKKEDHEGDGTRRYDLSSLKYLFLAGERCDPATYHWVKALLQIPVIDHWWQTE
                     TGWPMVGLMMGHDHLPSKAGSAGLPVCGYEIHILSEDGVELGKGKEGYIAAKLPLPPG
                     CLPTLWKNDDRFIAGYLQHFPGYYQTGDGGYLDDEGYCYIMGRVDDVINVSGHRLSTG
                     EMEELVASHPAIAECAVMGIADELRGQRPIALVVLKDGHQIEEEIIEEELVALIREKI
                     GAVAYFRNAAIAKRLPKTRSGKILRKTIRQIADGESFALPSTIDDPKILDEIREILEE
                     RKLGIAFNKV"
BASE COUNT          879 a          793 c          742 g          890 t
ORIGIN      
        1 gatgataacg atttcagccg cgataaactg tccgatattc aattgctgat tgacgagtaa
       61 gagggaccca atgatcagca tggccgcggt gataagtact ttaaaagcga tcaatacttt
      121 aaactgaaac tgaaggatat tgaaatggct gttgcgggca tccagatagc cgctgacttt
      181 ctcatccgtt ttccggagat tgatgaccga tcctttggaa aatttgaacg acttgatcag
      241 ccggcccatt tcctccagcc aggcagccac gccgtactta tatgcacttt ccgtcagact
      301 ggtctgtaat cctttattac cggtataata caggatcagc cagaggatca gcaccaggat
      361 catcccgaat aatataaagg ccggatgata aaaggagagc aggatcagtc cgaacacgat
      421 ttgtattgta gcagtaggta tatccagcag gagtttggcc agcccctttt gtaaggccgg
      481 tatttcaaaa aaacgattga ccagctcagg gatataatag gcgtccgttt ttttaagatc
      541 gagccttggt atcctgtccg ccagctcaaa ggagtatcgg acaaaaatat tttgctgaat
      601 tttttcaatc agctgcattt gtcccacctg caataatccg gtgaaaaata caccggcaac
      661 caccagtccg atgagtatga ccagtgaggt ggacatggaa ccgcctaata caaatccaat
      721 aattgcctgc acccctacgg gcagtgataa ctggatcagt ccattcagga tggcataaaa
      781 ataaatagca gatatttctt tacgttccag tttgaccagg cggaaaatcc tggaaaccgg
      841 gttagcagca gcactcatac aatgaggtta ataagtttga aaaaatggaa tatcgggcat
      901 tggtctgccc atgaatctat tgccggatca gcaattagga ggtggcgtta gttgttccag
      961 gctgggggct ggaagtattt tttctgtcag gtgaaatgca atagtcagcc gttggctgag
     1021 tatggctgag agctggcaga agtcggaata ttctttatta aatttctgat ttccatccac
     1081 ggttttctcc ccgtttttct cttcatccac attaaaaata accgaacaga cctggttact
     1141 aaacatggct tgtagcccgg gaataacctg gacaatggcg aagaggagta atatgaacag
     1201 ggctatcttt ttcatgtgtt tacgcctgca tataacatta ataaaagcag attgttttac
     1261 taaaagcccg ttttttataa aaaatatttt acagtgcaag tgttttaata tcaatcaatt
     1321 atactttatt gaaggctatt cctaattttc gttcctccag gatttccctg atttcatcca
     1381 gtattttcgg gtcatcaatt gtcgaaggga gggcaaagga ttctccatct gcaatctgac
     1441 ggatggtctt cctgagaatc tttccactcc gggttttagg taaacgtttt gctattgcgg
     1501 cattccggaa ataggccacc gctccgatct tctcccggat caatgccacc agttcctctt
     1561 caatgatttc ctcctcgatt tgatggccgt ctttcagtac caccagggca ataggccgtt
     1621 gtccacggag ttcatctgca atacccatta ctgcacattc tgcaatggcc ggatgtgaag
     1681 ccaccagttc ttccatttct ccggtactca accggtgtcc gctcacattg atcacatcat
     1741 ccacccgtcc catgatataa caataccctt catcatcgag ataacctcca tctccggtct
     1801 ggtaataccc ggggaaatgt tgcaggtaac cggcaataaa gcggtcgtcg ttcttccata
     1861 gtgttggcag acaacccggt ggcaggggca gtttagcagc gatatacccc tctttccctt
     1921 ttcccagttc cacgccatct tcggaaagta tatgtatttc atagccacaa accggcaaac
     1981 ccgctgatcc ggccttggat ggaagatgat catgtcccat catcaggccg accatcggcc
     2041 agccggtttc tgtctgccac caatgatcga tcaccggtat ttgcaacagg gctttgaccc
     2101 agtgataagt ggcggggtca caacgttccc cggcgagaaa aagatatttt aaagaactga
     2161 ggtcataacg ccgggtacca tctccttcat gatcttcttt tttaatcgcc cgtatggcag
     2221 tgggggcggt aaacatcaca ttcaccccat gttcactaat tacgcgccag aaggctgctg
     2281 catccggagt acgaatgggt tttccttcat aaagaatcgt ggtacaaccc tgcagcaatg
     2341 ggccatatac aatataacta tgaccgacta cccagcctac atcactggcc gcccagtata
     2401 cttcaccggg tttggcatca taaatatgat ccatgctgaa cttaagggcg gtagcatagc
     2461 cgccatgatc acggataatg cccttgggtt ttccggtggt gccggaggta taaaggatgt
     2521 ataaagggtc gccggaaaaa acgggcacac aaggaaccgg ttcagacaat agcaactggt
     2581 tgtaatccat ttcactgatg ccatccagtt cgtcgtaacc gggagttcgt tcataaaata
     2641 tatgcaccgg gggtttatgc agggcttctt caatggccct gtccactaat ggtttgtaag
     2701 gaatggtttt atcaaattcc acaccataac tcgccgatat gattgctttg ggaagggcat
     2761 cgtcaatccg catcgcgagt tcgtgcgggg caaaaccgcc gaatacgacg gagtgaattg
     2821 cgccgatccg cgcacaggcc aacattgcaa tcaccgcctg tggaatcatt ggcatgtaga
     2881 taaccaccgt atctccctta cggatgccag ctgattgtaa cccaccggca aagcgggaaa
     2941 caagatccag taactcctgg taactgtatt tttttatcgt atgtgtaacc ggtgaatcat
     3001 agatcagggc aatctgttcg ccccggccct gttccacctg ctgatccagg cagagataac
     3061 aggtgttgat ttccccatcg gcaaaccacc ggtaaaagcc tttttcattt tgggagagaa
     3121 tggtttccgg ggctttaaac caatgcagtt gtgccgcctg ttctttccag aagacttctt
     3181 tattgtcgat actctgccgg tatatcgcgt tgtatttttc catgctgcaa ttcgttgcta
     3241 caaattacaa ccggaagcgg agggttgaga caaacctata ccaattaacc agaattttat
     3301 ataa
//
LOCUS       DAOPMW010000386         3281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_35158_length_3281_cov_4.112523, whole genome shotgun sequence.
ACCESSION   DAOPMW010000386 DAOPMW010000000
VERSION     DAOPMW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35158_length_3281_cov_4.112523"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLU11_13965"
     CDS             <1..1322
                     /locus_tag="PLU11_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60287.1"
                     /translation="LYSNLAMVHSKLGHIDSARYYIDVAIRDARLNNNLVFLANALSM
                     QARIFIDNHQAPLAEQPLHEALMIRKKINDPFYIVYDMSSLAAYYAANNQPEKGIRLG
                     KEGVALAMESRLTSQLMMIYQALADNYKAAGLNESYGETLEKILRLKDSFNNINSSKM
                     LADLQANREAVRQEKLISEQKLNLTLKNYWLAGTILFSMMAATIGLLLFRHYSRKKKL
                     EMENALREEKRNAEAAIRNAEEKERVRIAADLHDNLGAYAASMASNLNYIRIQENDPL
                     SAAAFNELKNNSGSVISQLNDTIWVLRKENLSLTALSDRVKLFISRIGRSYPDIRLEV
                     SEQIGKDHQLSAAQAFHLYRVVQEGVNNALKHSGASAIIVNIESNDRWKISITDNGKG
                     FDPGLRANGGGHGLHTMRERSRESGWDIRWRVHPTGGTVVEMESTTN"
     gene            1362..2036
                     /locus_tag="PLU11_13970"
     CDS             1362..2036
                     /locus_tag="PLU11_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN60288.1"
                     /translation="MNFNIALTEDNSINRNTFLQKIRTMDLLNLVFVANNGKQCLEEL
                     KGLPVARLPQVIFMDLEMPEMNGIETIRLAKSLHPSIHFIVLTVFDEEEKIFEAIRAG
                     ASGYLLKHEPASVLQEAVTNVMEYGGAPMSPAVARKALQLMSRADVPGIVEDKNAIPS
                     MITSREEEILKHMVKGWDAKRIGVELNISVLTVRKHIANVYEKLHVQSKAEVISMAHQ
                     QNWFRS"
     gene            complement(2039..2488)
                     /locus_tag="PLU11_13975"
     CDS             complement(2039..2488)
                     /locus_tag="PLU11_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60289.1"
                     /translation="MPINVRRAVKEDCPRLLELVKELALYEKAPDEVTVTMEHFVESG
                     FGEKPVWWAFVAEEEGFIYGFALYYIRYSTWKGQAMYLEDIVVTEKARGKGIGKLLFD
                     RLMEESREKGFKRMVWQVLEWNEPAINFYKKYKAEFDGEWLNGSIYP"
     gene            complement(2497..>3281)
                     /locus_tag="PLU11_13980"
     CDS             complement(2497..>3281)
                     /locus_tag="PLU11_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN60290.1"
                     /translation="KLAMHNLWGKADSLLSIMDQARKEGVNITSDIYPYAFWHSTIRV
                     LFPDRNFTDEKEAEMILRDITLPGDIILAAYEIDPAYEGKTLAAIAALENKTPARMLI
                     ELIARIEAWEKTHNDECRESIMATSMAEQDITRLMQWPYANICSDGSSSGRHPRGYGA
                     FTRVLSKYTREEKALTLAGAIYKMTGLAATNLSIEKRGFLRPGYYADIVLFNPETVKD
                     LASVEQPHALSEGILQVWVNGQPVYQDKKGSGLFPGQLIKRK"
BASE COUNT          895 a          737 c          800 g          849 t
ORIGIN      
        1 ccttgtatag taacctggcg atggtacata gtaagctggg acacattgat tctgcccggt
       61 attatattga cgtggcgatc cgggatgcaa gactgaataa taacctggtc tttctggcca
      121 atgcattgag tatgcaggca agaattttta tagataatca tcaggcaccc ctggccgaac
      181 aacctttgca tgaagcattg atgatccgga aaaaaataaa tgaccccttt tatattgttt
      241 atgatatgag cagcctggcg gcgtattacg ccgccaataa tcagccggag aaaggaatca
      301 ggttgggtaa agagggcgtc gccctggcaa tggaatcaag actcacttca cagctgatga
      361 tgatttacca ggcactggca gataattaca aagcagccgg attaaatgag tcatatgggg
      421 agacattgga aaaaatcctg cggctgaagg actcttttaa taatattaat tcttccaaaa
      481 tgctggcgga cctccaggca aaccgggaag cagtaagaca ggagaaactg atcagtgagc
      541 aaaagctcaa ccttacgtta aagaattact ggctggcagg tactattctt ttttcaatga
      601 tggcggccac tattggtttg ttgttattcc gtcattacag ccggaagaaa aaactggaaa
      661 tggaaaacgc cctgcgtgaa gaaaaaagaa atgcggaagc cgcgatccgc aatgcagaag
      721 aaaaggaacg ggtccggatc gcggccgacc tgcatgataa cctgggtgct tatgccgcct
      781 ctatggcttc caacctgaat tatatacgga tccaggaaaa cgatccgtta tccgctgccg
      841 cattcaatga attaaaaaat aattccggat cagttatttc ccagttgaat gacacgatct
      901 gggttttacg aaaggaaaac ctgtcgctaa ctgctctcag tgaccgggta aaattattta
      961 tcagccgcat tgggcggagt tatccggaca tccgcctcga agtttcggaa caaatcggga
     1021 aggatcatca gctgtctgcc gcacaggcct ttcatttgta ccgggtggta caggaagggg
     1081 tgaacaatgc attaaaacac agcggcgctt ctgcgattat tgtaaatatc gaatcgaatg
     1141 atcgatggaa aataagtatt acagataatg gaaaagggtt tgatcccggt ctgcgggcta
     1201 atggcggagg acatggatta cataccatgc gggaaaggag ccgggagtcg ggctgggata
     1261 tccgctggcg tgtgcatcca acgggaggta cggtggtgga aatggagtct actacaaact
     1321 gattatttac tatccggaaa gggtccggta aattgcagga tatgaacttc aatatcgcct
     1381 taacggaaga caacagcatc aaccggaata cttttttaca gaagatcagg acgatggacc
     1441 tgctgaacct ggtatttgtg gccaataatg gaaagcaatg cctggaagaa ctgaaagggt
     1501 taccggttgc ccggttgcca caggtgattt ttatggatct ggagatgccg gagatgaatg
     1561 gtattgaaac aatccggttg gccaaatccc tgcatccctc catacatttt attgtactga
     1621 ctgtttttga tgaggaagaa aaaatatttg aagccatccg ggccggggct tccggttact
     1681 tgctaaaaca tgaaccggcc agcgtgctgc aggaagcggt gaccaatgtg atggaatatg
     1741 gcggggcacc catgagtccg gcagtggccc ggaaagccct tcagttaatg agccgggccg
     1801 atgtaccggg tatagtagaa gataaaaatg ccattccttc catgatcacc agccgggaag
     1861 aggaaattct gaaacacatg gtgaaaggat gggatgccaa gcggattggg gtggagctga
     1921 atatcagtgt gctcaccgta cgcaagcata ttgcgaatgt gtatgaaaaa ctgcatgtgc
     1981 aatccaaagc agaagtcatc agcatggccc accagcaaaa ctggttcagg tcttaggttc
     2041 agggataaat ggatccgttc agccattcac catcaaattc tgccttatat tttttataaa
     2101 aatttatagc aggttcattc cattccagca cctgccatac cattcgtttg aacccctttt
     2161 ccctggattc ctccatcaaa cggtcaaaca acaatttgcc aatacctttg ccccgggctt
     2221 tttcagttac aacaatatct tccaggtaca tggcttgtcc cttccaggtg gaataacgga
     2281 tataatagag ggcaaaacca taaatgaacc cttcttcttc agccacaaag gcccaccata
     2341 ccggtttttc gccaaatccg ctttctacaa aatgttccat cgttaccgtt acttcatccg
     2401 gtgctttttc gtacagggcc agttccttta ccagctccag taaacggggg cagtcttctt
     2461 tcactgctct tcgtacattg atcggcatag cttttcttat ttacgtttta tcagctgacc
     2521 cggaaagagg ccgcttcctt ttttatcctg gtaaaccggc tgtccgttca cccatacctg
     2581 taggatgcct tctgataaag catgtggttg ttctacggag gccaggtctt tgactgtctc
     2641 cggattaaag aggacgatat ccgcataata acccgggcgg agaaatcctc ttttttcaat
     2701 ggacaggttg gtggcggcca gcccggtcat tttgtaaatg gccccggcca gggtcagtgc
     2761 tttttcctcc cgggtatatt tggataatac acgggtaaaa gcaccgtaac cacgtggatg
     2821 tcttccggag gatgaaccgt cggaacagat attggcatag ggccattgca tcagccgggt
     2881 tatatcctgt tctgccattg atgtagccat gatactttcg cggcattcat cattatgagt
     2941 cttttcccag gcttcaatcc gggcaatcag ttcaatcagc atccgcgcag gggttttgtt
     3001 ttccagtgcg gcaatagcgg ccagcgtttt tccttcatag gccggatcaa tctcataagc
     3061 agcaagaata atatcaccgg gtagtgtaat atcccggaga atcatttccg cttctttctc
     3121 atctgtaaag ttgcgatcag gaaacaagac cctgatggtt gaatgccaga atgcatacgg
     3181 ataaatatca ctggtgatgt tcacaccttc ttttctggcc tggtccatga tggacagcag
     3241 gctgtctgct tttccccaga gattatgcat ggccagtttt a
//
LOCUS       DAOPMW010000387         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_35480_length_3260_cov_5.735101, whole genome shotgun sequence.
ACCESSION   DAOPMW010000387 DAOPMW010000000
VERSION     DAOPMW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35480_length_3260_cov_5.735101"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLU11_13985"
     CDS             <1..768
                     /locus_tag="PLU11_13985"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN60291.1"
                     /translation="LFPESGITFDTPMVVNFNLHGQKFMGLNGGPMFKFNPSISMFVT
                     SETNEEIDALYAKLIEGGMVMMPLDKYEWSEYYAFVQDKFGLAWQLYKGSYSAVNQKI
                     TPCFLFTDDHFGKAEAAVNYYTALFPSSSVNGILKYQDNEGAEVAGKVKHSQFIVDDK
                     VFMAMDGAGNHGFSFNEAISFVVECKDQEEIDHYWNYMTSNGGSESMCGWLKDPFGVS
                     WQIIPAKMGQIMTDSGKGAGAMKALMQMKKIVIAELG"
     gene            1009..1386
                     /locus_tag="PLU11_13990"
     CDS             1009..1386
                     /locus_tag="PLU11_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPN60292.1"
                     /translation="MKQKTTTILYWVFTLLFSAFMIWSSYSGINPKGQTLEFLNGYLG
                     YPVYFIQFISVAKIIGAVVILVPGLNKIKEWAYAGLFFDLAGAIFSIYMVQKKFDTGV
                     LFMLVPVLLGAASYYFWNKQKIS"
     gene            1430..1954
                     /locus_tag="PLU11_13995"
     CDS             1430..1954
                     /locus_tag="PLU11_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPN60293.1"
                     /translation="MIFVYILAAIIAAFFLTALLVKKDFSLEKQIVINKTQKEVFDYL
                     KLIRNQEKYSVWVMRDPHINLVYTGTDGTVGFKSAWTSNDKNVGIGEQEITKLTEGVS
                     TEVEIRFKKPFEATNWAKTTVTAVSEQETRVHQLFYGRSKFPMNIMNLFMDKLLGKDM
                     LQNLVNMKKNLEEN"
     gene            1973..2749
                     /locus_tag="PLU11_14000"
     CDS             1973..2749
                     /locus_tag="PLU11_14000"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPN60294.1"
                     /translation="MKSTAPAKTPDQYIAGFPATVKKRLEEIRKTIRDNAPDAVEGIS
                     YGMPGFKYLGKPLVYFAGYENHIGFYATPTGHEAFKKDLAGYKTGKGSVQFPLTEPLP
                     LALIKKIVLFRVKENEGMAGKKKTTVTAVKKKAAPENKEAAQVKEWLDKQEPAVRKEI
                     DTVRKLIKKAAPTLNERIKWNAPSYYTTADLFTFGPYKNKRILLVFHHPAIVEIKSPL
                     LEGEYKDRRLVYFNSSKEAVKNEKELSRIIRLLTNKAGNH"
     gene            complement(2804..>3260)
                     /locus_tag="PLU11_14005"
     CDS             complement(2804..>3260)
                     /locus_tag="PLU11_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN60295.1"
                     /translation="DEKSYELNLRLGWLTYLNKEYTTSQSYYQKAVNLRPYSLEAKFG
                     LIQPLAALQLTDKTLLVYEDILKIDPQQTKANYWVGVIWYNRKKYETAARYFEKLVNL
                     YPFDYDGNHMLAWTYLNMGRSNDAKLLFGKALLAKPGDASCLEGLKQVR"
BASE COUNT          980 a          758 c          714 g          808 t
ORIGIN      
        1 ttgttcccgg aatccggtat tacgttcgat acgccaatgg tagtgaactt caacctgcac
       61 ggacagaagt ttatgggact gaatggcgga ccgatgttta aattcaatcc atccatttcc
      121 atgtttgtca ccagtgaaac caacgaagag attgatgcgc tctatgcaaa actgattgag
      181 ggcggtatgg tcatgatgcc actggataaa tatgagtgga gtgaatacta tgccttcgta
      241 caggataaat tcgggctggc ctggcaatta tataaaggaa gctacagtgc cgttaatcag
      301 aagattaccc cctgcttctt atttacagat gaccatttcg gtaaagcgga agcggccgtg
      361 aattattata cagcgctttt cccttcctct tcggtcaatg gtatcctgaa ataccaggac
      421 aacgagggag ccgaagtagc aggtaaagtc aagcattccc agttcatcgt tgacgacaaa
      481 gtcttcatgg ccatggatgg cgccggcaat catggttttt cttttaatga agctatttcc
      541 tttgtggtgg aatgtaaaga ccaggaagag atcgatcatt actggaatta tatgaccagc
      601 aatggcgggt cggaaagcat gtgcggctgg ctcaaagatc cctttggagt gtcctggcaa
      661 atcatccccg ccaaaatggg acaaatcatg acggattccg gaaaaggcgc cggagcgatg
      721 aaggcgttga tgcagatgaa gaagatagtg atagcggagc ttggttaaaa tgccctacgc
      781 cagttgaaat aaggaactgt acattgtccc ccgctggcgg gggaagtgga tcgagcagaa
      841 tgtaactttc aaataggggt ggataaaaga aactctacct atccacccct atttgaaaaa
      901 aattttaatc cgaacacccg gcccccgcca gcgggggata acgcaaagtc attcaatccg
      961 gattgcgtaa tttgatggac taattatctt taattaattg atatcaccgt gaaacaaaaa
     1021 acaacaacaa tcctctactg ggtcttcacc ctcctcttct cggccttcat gatctggtct
     1081 tcttattcag ggataaaccc caaaggacag acccttgaat tcctgaatgg ctacctcggg
     1141 taccctgttt actttattca gtttatcagt gtggcgaaga tcatcggcgc cgtggttatc
     1201 ctggttccgg gactcaataa aataaaagaa tgggcctatg ccggtctttt cttcgacctg
     1261 gccggtgcga tcttctccat ttatatggta cagaaaaaat tcgatactgg cgttttattc
     1321 atgctcgtcc cggtactcct gggagcagct tcctattatt tctggaacaa acagaaaatc
     1381 tcctgatccc caattcctaa tcctccattc accaatcccc caattaacca tgatcttcgt
     1441 gtatatcctc gccgccatca tcgctgcttt tttcttaacc gctttgctgg taaaaaaaga
     1501 tttctctctc gaaaagcaga tcgtgatcaa taaaacacag aaggaggtat tcgattacct
     1561 gaaactgatc cgcaaccagg agaaatacag tgtatgggta atgcgggacc cccatattaa
     1621 tctggtgtat accggaaccg atggcacggt cggattcaaa tcggcctgga ccagcaatga
     1681 taaaaatgta gggatcggtg aacaggaaat cactaaacta acagagggtg tcagcaccga
     1741 agtggaaatc cgtttcaaaa aacccttcga agccaccaac tgggcgaaga cgacggtgac
     1801 cgcggtgagt gaacaggaga cccgggtgca ccagttattc tatggacgca gcaagttccc
     1861 catgaatatc atgaacttgt ttatggataa actgcttgga aaagatatgc tgcaaaacct
     1921 ggtgaatatg aagaagaacc tggaagaaaa ttaaaccttt caattttatt ttatgaaatc
     1981 aacagctcct gccaaaacac cagatcagta tatagccggt ttcccggcca cggtaaaaaa
     2041 acgcctggaa gaaatacgca aaactattcg tgacaatgca ccggatgcgg tggaagggat
     2101 cagttatggt atgcccggtt tcaaatacct gggcaaaccg ctggtgtact ttgccggcta
     2161 tgaaaaccat atcggttttt atgccacgcc caccggtcat gaagctttta aaaaagacct
     2221 ggctggttat aaaaccggga aagggtcggt acaatttcca ttgacagaac ccctgcccct
     2281 ggccctgatt aaaaagatag tgctgttccg ggtaaaagaa aatgaaggaa tggccggcaa
     2341 aaagaaaact acagtaacag cggttaaaaa aaaagcagcg cccgaaaata aagaagccgc
     2401 ccaggtaaag gaatggctgg acaaacagga acccgctgtg cgtaaagaaa ttgataccgt
     2461 ccgaaaactg atcaaaaaag cagcgcccac cctgaatgaa cggatcaaat ggaatgcacc
     2521 cagttattac acaactgccg atttattcac cttcggtcca tacaaaaaca aacgcatcct
     2581 gctcgtcttt caccatccgg cgattgttga aatcaaatcc cctttactgg aaggagaata
     2641 caaggaccgg cggctggtgt atttcaacag cagcaaagaa gcggttaaaa atgaaaaaga
     2701 actgtcccgc atcatccgcc ttttaacaaa caaagccgga aaccactaac ccataacaaa
     2761 cagctcacaa cttataactt aaaacttttt acttacgact tacttacctg acctgcttca
     2821 acccctccag acaagaagca tcacctggct ttgccaataa agctttacca aaaagcagct
     2881 tcgcatcatt actccggccc atgttcagat aagtccacgc cagcatatga ttgccatcgt
     2941 agtcaaaggg atataggttg accagttttt caaaatacct ggctgctgtt tcatattttt
     3001 tccggttata ccagatcacc ccaacccagt aattggcctt ggtctgttgc ggatcaatct
     3061 tcaatatgtc ttcataaacc agtaaggttt tatccgtcag ttgcaatgca gccagtggct
     3121 gtatcagtcc aaatttagcc tctagcgaat aaggacggag gttaacggct ttctgataat
     3181 aagactgtga tgttgtatac tccttgttca gataggtgag ccagcccaac cggagattca
     3241 gttcataact cttctcgtca
//
LOCUS       DAOPMW010000388         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_35741_length_3242_cov_9.776906, whole genome shotgun sequence.
ACCESSION   DAOPMW010000388 DAOPMW010000000
VERSION     DAOPMW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35741_length_3242_cov_9.776906"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /locus_tag="PLU11_14010"
     CDS             complement(<1..2111)
                     /locus_tag="PLU11_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HPN60296.1"
                     /translation="MRKNKPNRTRISKCLLMICCCTCWIFSSHAQLVSTGKTGFDLTG
                     ALIYTDPAEDILVKKSAEFLQQDIERVTGRKPAIVSQYEKTKALYIIIGTTGKSGLIH
                     QLSVSGKIGLKEITGKWEAYKIQTITGIPGQPAAKALVIAGSDRRGVSYGVLELSRQM
                     GVSPWYWWADVPVQKKPAIYINTSVIINDAPKVKYRGIFINDEAPCLSTWSKEKFGGF
                     NHVFYEHVFELILRLKGNYLWPAMWGNAFYDDDSLNRKSADEYGIVIGTSHHEPLMRA
                     HDEWRRYGKGKWNYDSNEVRLKEFWKSGMQRAWNEKIVSVGMRGDGDEPMSRETATAL
                     LERIVKDQREIIAEVTGKPAGETPQLWALYKEVQDYYDKGMRVPDDITLLLCDDNWGN
                     IRKLPQHGEKPREGGYGLYYHFDYVGGPRNYKWINTNPIPRIREQLHLAWSYNVKQIW
                     IVNVGDIKPMEFPISFFLDYAWNPEKIGPADVKKYTEQWAAAQFGKKQAPVIGELLSG
                     YAKFNSRRKPEMLSDNTYSFNYGEWPRVVREYTALAEKAEKIGQRLPSDYQDAYFQLV
                     LHPVKASANLYQLYYNVALNKQAYANKWLSANHYADKVKEFYANDSLITLAYHQLNNG
                     KWNHMMSQTHIGYTSWQQPIRQKMPMVNYVNESAAAAETDTTSISRKAKFPANTPYPV
                     FYEQSQYVSIGADHYTKAVNS"
     gene            complement(2130..>3242)
                     /locus_tag="PLU11_14015"
     CDS             complement(2130..>3242)
                     /locus_tag="PLU11_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPN60297.1"
                     /translation="LTEWTDHGMIITQNNVPWVRPDSYSMWAPDCIERNGKYYFYFPS
                     APADTLAYGKGFTIGVAVADKPEGPYTAEITPINGVRGIDPNVFIDKDGQAYLYWSAG
                     NIYGARLKENMKELDSEVKILGDLPNKGLKEGPFLFEREGVYYLTYPHVANKTEQLEY
                     AMADSPLGPFKVTGVIMDESPTGCWTNHQSIIQFNNQWYLFYHHNDYSPSFDKARSIR
                     ADSLFFNEDKTIRKVIPTLRGIGITKATEKIQLDRYSAISPQGTAIEYLDTNNRFAGW
                     KTVLSNPGAWIQYNSVEFKNNKTATLHMNGEENGFIEIRTGKPDGPLIAKMLVVKGSS
                     MNTINAALRKRISGQHNLFVVSKCNKPVYIDWLSFQ"
BASE COUNT          749 a          754 c          746 g          993 t
ORIGIN      
        1 ttgctgttga ccgcttttgt ataatgatct gccccgatgg agacatactg actctgttca
       61 taaaaaacag gataaggggt attggccgga aattttgctt ttcgggatat tgaagtggta
      121 tcagtttcag cagcagcagc tgattcattg acataattta ccattggcat tttctgacgg
      181 atcggttgtt gccagctggt gtagccaata tgggtctggc tcatcatatg attccacttt
      241 ccgttgttta actggtggta agctaacgtg atcagggaat cattggcata aaactctttt
      301 actttatcgg catagtggtt ggcggataac catttattgg cataagcctg tttgtttaag
      361 gccacgttgt aataaagttg gtaaagattg gcacttgctt tgaccggatg caataccagc
      421 tgaaaatagg catcctggta gtccgatggt aatcgttgac cgatcttttc agctttttca
      481 gctaatgccg tatattctct taccaccctt ggccattctc cataattaaa actatacgta
      541 ttatcgctga gcatttccgg ttttctccgg ctgttgaatt tggcatagcc ggacaagagt
      601 tcgccaatta ccggggcctg ttttttgccg aactgggcag cggcccactg ttctgtatat
      661 tttttcacat ctgcagggcc aatcttttcc ggattccagg cataatcaag aaaaaaagaa
      721 atgggaaact ccatgggttt gatatcgccc acgtttacga tccatatctg ttttacatta
      781 tacgaccagg ccaggtgcag ttgttcccgg atccggggaa tggggttggt gttgatccat
      841 ttgtaattgc gggggccgcc tacatagtca aaatggtaat acaaaccata gccaccttcc
      901 cggggctttt ctccgtgttg aggcaatttg cggatattgc cccagttatc atcgcataat
      961 aatagcgtaa tgtcatcggg tacccgcatg cctttgtcat agtaatcctg tacttcttta
     1021 tacagggccc aaagctgagg cgtttcaccg gcgggtttgc cggttacttc cgctatgatt
     1081 tcccgctggt ctttcacaat gcgttcaagc agggccgtgg cggtttcgcg actcatcggt
     1141 tcatccccat ctccacgcat acccaccgaa actattttct cgttccaggc gcgttgcata
     1201 cctgatttcc agaattcttt cagccggact tcattactgt cataattcca tttgcctttt
     1261 ccatatcgtc tccattcatc atgggcccgc atcaggggtt catgatggga ggtgccgatt
     1321 acaattccat actcgtccgc tgattttctg ttcagcgagt catcgtcata aaaggcgttt
     1381 ccccacatgg ccggccagag gtaattgccc ttcagacgca ggatgagttc aaaaacatgt
     1441 tcatagaata catgattgaa tcctccgaat ttttctttgc tccaggtact caggcagggg
     1501 gcctcatcat taataaatat gccccggtat tttacttttg gcgcatcatt gataatgacg
     1561 gaagtattta tatagatcgc cggctttttc tgaacaggta catcagccca ccaataccag
     1621 ggcgatacac ccatttgccg ggagagttcc aatactccat agctgactcc ccggcggtca
     1681 ctgccagcga tcaccagtgc tttggctgcg ggttgaccag ggatacctgt aatcgtttgt
     1741 attttataag cctcccattt gccagtaatc tccttcagcc cgatcttgcc ggaaacggaa
     1801 agttggtgta ttaaccctga ttttcctgtg gtaccaataa tgatatacag ggctttcgtt
     1861 ttttcatatt ggctgacgat tgcaggtttc ctgccggtaa cccgttcaat atcctgttgc
     1921 aggaattctg cggatttctt taccaggata tcttcagcag gatccgtata gatcaacgcc
     1981 ccggtaagat caaacccggt cttgccggtt gaaaccagtt gtgcatggct gctgaagatc
     2041 cagcaggtgc aacaacagat catcagcagg catttactga tacgggtccg gttcggttta
     2101 ttttttctca tacatgatcc gtttaaaagt tattgaaagc tcagccagtc gatatataca
     2161 ggtttgttgc atttggatac gacaaacagg ttgtgttgac cagaaatgcg ttttcgcagc
     2221 gcggcgttaa tggtattcat tgaacttccc ttcaccacga gcatttttgc gatcaatggc
     2281 ccgtctggct ttccggttct gatttctata aatccattct cttcgccgtt catgtggaga
     2341 gttgccgttt tattattttt aaactcaaca ctattgtact gtatccaggc ccccggattg
     2401 gaaagaacgg ttttccatcc ggcaaagcgg ttattggtat ccaggtattc aatggcagtg
     2461 ccttgcgggc taatggctga ataccggtca agctggattt tttctgttgc ctttgttata
     2521 ccgatgcccc ttaacgtggg gatcactttc cggatggttt tatcttcatt gaagaataaa
     2581 ctgtcggccc gtatggatcg ggctttgtca aaggaagggg agtagtcgtt atgatgataa
     2641 aacaggtacc attggttgtt aaactggatg atggattgat gattggtcca gcatccggtc
     2701 ggggattcat ccatgatcac accggttact ttgaatggcc ccagcggact gtctgccatg
     2761 gcatactcca gttgttctgt tttattggcc acatgagggt aggtgaggta gtatactccc
     2821 tcccgctcaa acaggaatgg accttctttt aatcctttgt tgggcaggtc accaagtatc
     2881 tttacttccg agtccagttc tttcatgttt tcttttaacc gggccccgta aatattacca
     2941 gccgaccaat agagataggc ctgtccgtct ttatcgataa atacgttggg gtcaatgcca
     3001 cggacaccgt tgatcggtgt tatttctgct gtatagggac cttccggttt atcggctaca
     3061 gccactccaa tggtaaaacc ttttccgtag gccaaggtat cagccggtgc ggaaggaaaa
     3121 tagaaataat atttgccatt tcgttcaatg caatccggag cccacatgct ataactgtcc
     3181 ggacggaccc agggaacatt gttttgtgtg atgatcatac catggtctgt ccattcggtc
     3241 ag
//
LOCUS       DAOPMW010000389         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_35818_length_3237_cov_4.082652, whole genome shotgun sequence.
ACCESSION   DAOPMW010000389 DAOPMW010000000
VERSION     DAOPMW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35818_length_3237_cov_4.082652"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PLU11_14020"
     CDS             <1..1552
                     /locus_tag="PLU11_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPN60298.1"
                     /translation="DYIDMALYGTKKDGKSGFIALMDGLFSPDGFYAEGPYYTRYAIL
                     PYYLLATALDKVRPELKIFQYRDQILQKALMAGLQQTNLDGTFYSFNDALKEKDYTSN
                     ELVTAVDIAWDKYGKDDGLLVVARAQNRVLLNKGGVSIAAALASGKNNTLRFPYKTVE
                     YRDGSNGDRGGVTVLRSGKDDQLSSLIFKYTSHGLSHGHYDKLNYQFYDKGNEILRDY
                     GAVRFIGIEQKYGGRYLPETKGYAAQTIAHNTLVLDEKSHFNGKEETAEKYPAQKRFS
                     QLKGPAVQVVSAGTDKAYDGVNLQRTVYLLDLPGGNRLVADLFTVESAAEHQYDLPFQ
                     YNGQLISTSVKYTPFTDKQETLGKKNGYQYLWKEAEARVNDTLVQLTFLNDRTYYSLS
                     TLVQGPATVFFTRSGANDPNFNLRHEPAMIVRNTGRNQTYVSVLEVHGQFDPINEFST
                     YAYASVKQIRLLRQDAQYTLAEIMLGEKRIIIAQSNSTTDKEASHSVSGYNWKGPYAV
                     YYNGIELR"
     gene            1579..1941
                     /locus_tag="PLU11_14025"
     CDS             1579..1941
                     /locus_tag="PLU11_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN60299.1"
                     /translation="MKEMESTEAARVFIDNNELDWQVVDTGVKRKIMSFDDRLMLVKV
                     SFDKGGVGTLHQHPHSQITHVESGVFEVEIGEEKKILKGGDAFYIPPNILHGAVCLEA
                     GILIDVFSPMREDFIDNK"
     assembly_gap    1943..2042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2109..3134
                     /locus_tag="PLU11_14030"
     CDS             2109..3134
                     /locus_tag="PLU11_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60300.1"
                     /translation="MLRLLCIYSLFFLIGCNKKNTPAQTPDPVPGPGGSSRVVNVSTA
                     AALNAALLDAKPGDEIIMADGIYKGKFVIGATVNGTATKPVILRGSRNVILDAETTTT
                     GYVLHLQASYWILRGFTLTNGLKGLMVDGARKSVIDSLKVFNIGEEAIHLRKFSSSNT
                     VQFCEISQVGLKTPDYGEGIYIGSAKNNWSTYTNGQPDFCDSNRIISNRIGPGITAEC
                     IDVKEGTTGGLIRGNYFDATGITGANSGDSWMDVKGNYYLIEENTGFNPGGPIFKDGF
                     QVHVAYPGWGNNNEFRNNECTVNATGYGFNIQLSGSNGSSTGNKVYVSNTVNGAQSGI
                     ANITLSN"
BASE COUNT          918 a          644 c          779 g          796 t
ORIGIN      
        1 ggactatatt gatatggcct tatatggtac aaagaaggat gggaaaagcg gctttatcgc
       61 cctgatggat ggcctgtttt cacccgacgg cttttatgcg gaaggtcctt attatacacg
      121 ctacgcaatc ctgccttatt atttattggc aaccgcattg gacaaagtac gtcctgaact
      181 gaaaattttt caataccggg atcagatttt acaaaaagca ttaatggccg gtttgcaaca
      241 aacaaacctg gatggcacat tttattcatt caacgatgca ctgaaagaaa aagattatac
      301 gtccaatgaa ctggtaacgg ccgtggatat tgcctgggat aagtatggaa aggatgacgg
      361 tctgctggtg gtggcccgtg cacaaaaccg ggtgctgctc aacaaaggcg gggtttccat
      421 tgccgctgca ctggcatccg ggaaaaataa tacactccgt tttccttata aaacagtgga
      481 ataccgcgat ggaagcaatg gggacagggg cggtgttacc gtattacgca gcggcaaaga
      541 cgatcagctc agcagcctga tttttaaata tacttcacat gggctttctc atggccacta
      601 tgataaactg aattatcagt tttatgataa gggaaatgaa attctccggg attacggagc
      661 cgtccgcttt atcgggatcg aacagaaata cggcggacgt tatttgcctg aaaccaaggg
      721 ctatgcggca caaaccattg cgcataatac cctggtgctg gatgagaaaa gccattttaa
      781 cggaaaagaa gagaccgcag aaaaatatcc ggcacaaaag cgattcagtc aactaaaggg
      841 ccctgccgta caggtggtct cagccggtac agataaagcc tatgacgggg taaacctgca
      901 gcgtacggtt tacctgcttg atctgcctgg aggtaaccgg ttggtggctg acttgtttac
      961 cgttgaatcg gcggctgaac atcagtatga tcttcctttt cagtataacg gacaactgat
     1021 cagtacctcg gtaaaataca ctccatttac agataaacag gaaacactcg gaaaaaagaa
     1081 cggctaccag tatttatgga aggaagcgga agcccgggtg aatgatacat tggtacaact
     1141 tacttttctg aatgaccgga cctattacag tttgtccacg ctggtacaag ggcctgccac
     1201 tgtttttttt acccgttccg gggccaatga tccgaatttt aatctccggc atgagcctgc
     1261 catgatcgta cgaaatacgg gtcgcaacca aacctatgtt agtgtattgg aggtgcatgg
     1321 tcagttcgat ccgatcaacg aattttccac ctatgcctat gcatcggtaa aacagatccg
     1381 tctcctcagg caggatgcgc agtacaccct tgcggagatt atgttgggtg agaagcggat
     1441 cattattgca cagtcaaaca gcaccaccga taaagaggct tctcacagcg tttcaggata
     1501 taactggaaa gggccctatg cggtgtacta taacgggatt gaattaagat aagtcgttaa
     1561 ttatataata ccattattat gaaagaaatg gaatcaactg aagcggccag ggttttcatt
     1621 gataacaatg aactggattg gcaggtggtc gatacaggcg taaaaagaaa aatcatgtca
     1681 tttgatgacc ggctgatgct ggtaaaagta tcgtttgata aaggcggggt gggtactctc
     1741 caccagcacc cgcattcaca gatcacccat gtggaaagcg gggtgtttga agtggaaatc
     1801 ggggaagaga aaaaaatcct gaaagggggc gatgcctttt atatcccacc caatatactt
     1861 catggagccg tctgcctgga agccggaatt ttaattgatg tattcagtcc gatgagagag
     1921 gattttatag ataataaata acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnattttaat tgatgtattc agtccgatga gagaggattt tatagataat aaataacggg
     2101 attgtccaat gctgaggttg ctatgtatat acagtctttt ttttctgatc gggtgtaata
     2161 aaaaaaatac accggcccag acgcctgatc ctgtacccgg tcccggtggt tcttcccggg
     2221 tggttaatgt gtcgaccgca gcggccctga atgccgcctt gctggatgca aagccgggtg
     2281 atgaaattat catggcggat ggcatctaca aaggcaaatt tgtaatcggg gcaactgtaa
     2341 atggtacggc gacaaagccg gtcattcttc gcggcagccg gaatgtaatc ctggatgcag
     2401 aaaccaccac aaccgggtat gtgctacacc tgcaggccag ttactggata ttgagaggct
     2461 ttaccctcac caatggtctt aaagggctca tggttgacgg agcccgtaaa tcggttattg
     2521 atagtctgaa agttttcaat atcggtgaag aagcgataca tctccgcaaa ttcagttcat
     2581 ccaataccgt acagttttgt gaaatcagcc aggtaggatt aaagaccccc gattatggag
     2641 agggtattta tatcggttca gcaaaaaata actggtccac ttataccaac ggacaaccgg
     2701 atttctgcga ttccaaccgg atcatcagta accggatcgg ccccggcatt acggcggaat
     2761 gtattgatgt aaaagaagga accaccggtg gactgatccg tggaaattat tttgatgcca
     2821 ccggtattac gggtgccaac agcggcgaca gctggatgga tgtgaaagga aactattacc
     2881 tcatagaaga gaataccgga tttaatcccg gcggccccat tttcaaggat ggtttccagg
     2941 tacatgtagc ttatccggga tggggtaata ataatgaatt cagaaataat gaatgtacgg
     3001 tgaatgccac gggctatggt tttaatattc agctgagcgg aagcaatggc agttccaccg
     3061 ggaataaagt atatgtcagc aacacggtaa atggtgcaca aagcggtata gcaaatatta
     3121 cgttgagtaa ctgaaaatta tgaaagtaaa aggtttacgt tggtatatta tcgggctcat
     3181 cgggctggca acgatcatta attatattga tcgtacggcc attaatatac tttggcc
//
LOCUS       DAOPMW010000390         3223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36062_length_3223_cov_3.030934, whole genome shotgun sequence.
ACCESSION   DAOPMW010000390 DAOPMW010000000
VERSION     DAOPMW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36062_length_3223_cov_3.030934"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLU11_14035"
     CDS             <1..1007
                     /locus_tag="PLU11_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60301.1"
                     /translation="TLRFNTDPNITEFEERELLIRYIKLGLAPFIARTAAAKDITISY
                     KSGKTDSTVKTVTKLTKDPWNYWVYRVGANGNMDADEVYKNFRYSVNLSANRTTEEVK
                     LGFSMSGSRNRSAFEYDDGTGLIKYVVNNHNFSMGHYLVKSINSHWSWGYETNYSQNT
                     FSNNRGRIFLRTALEYNIFPYKEVNTRSFTLSYGITARQNRYYDTTLYNKLKENLYGH
                     GFNAHMTYNQKWGTSYIGVNYHNYFHDWKYFNLGMEAYTSVRVTGGLSFYISAFGGLS
                     RDQFYLQKGSATSQEVLARQRQLASGYSYYFNLGINYRFGSKLNNFVNPRFDGTSNSN
                     "
     gene            complement(1017..2321)
                     /locus_tag="PLU11_14040"
     CDS             complement(1017..2321)
                     /locus_tag="PLU11_14040"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPN60302.1"
                     /translation="MKPVVTVFWFRRDLRLHDNAGLYQALQSGQPVLPLFIFDTEILD
                     SLEDKDDRRVEFIHRAISVMQEQLTTMGSTLDVRYGKPLQVYQDLLNEYEIKAVYTNH
                     DYEPYARQRDQAIAQWLKTKDIQFHSFKDHVIFEKDEVLKDDGKPYTVFTPYSRKWKA
                     VISTHPIEVFNTKKFYKHFLSQQPMTVPSLAAMGFTAAGLPFPAKSWKKEVITQYKEQ
                     RDIPSLPGTSRLSVHLRFGTISIRELAREAGVLNETFLNELIWRDFYHMILWHFPQVV
                     GHSFKPDYDKIRWRNKETEFDAWCKGQTGYPIVDAGMRELNATGFMHNRVRMIVASFL
                     TKHLLIDWRWGEGYFARKLLDFDLSANNGGWQWAAGSGCDAAPYFRVFNPYLQTQKFD
                     PELKYIRQWVPEFEELTYVRPVVVHEVARKRCLEVYAAALKK"
     gene            complement(2337..3173)
                     /locus_tag="PLU11_14045"
     CDS             complement(2337..3173)
                     /locus_tag="PLU11_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPN60303.1"
                     /translation="MYISFNGKITPADQPVLLASNRSYRYGDGLFETMKLDQGKILLE
                     ELHFQRLLTGLELLKFDRPKLFSTSKLAAEIIQLAEKNQVRDLGRVRLSVFRGNGGLY
                     DADRTLQYLIECWPLPESFSRLNENGLEIGIYPDAPKQISLFSGLKSANFLPYTLASI
                     YAKENKLNDCLLLNQEGHIADSTIANLFVYLEGKFITPGPEQGAVDGVMRKYLVREMR
                     AAGYTVEEGPVNIAALEAANEVFLSNAIHGVKWVRRFRDSIYSNKYVSELYSRFIQTI
                     PR"
BASE COUNT          899 a          759 c          712 g          853 t
ORIGIN      
        1 acacgcttcg ttttaatacg gatccgaata tcacagaatt tgaagaacgg gaattactga
       61 tccggtatat caaactcggg ctcgctcctt ttatcgcccg tactgcagca gcaaaagata
      121 tcacgatcag ttataaatcc ggcaaaaccg attcaacggt aaaaacagta accaaactga
      181 ctaaagatcc ctggaattac tgggtatacc gggtgggcgc aaatggaaat atggatgcag
      241 atgaagtcta taaaaatttc aggtacagcg tgaatctctc cgccaaccgg actacggaag
      301 aggtgaagct tggtttcagc atgagtggca gccgcaaccg ctctgctttt gaatatgacg
      361 atggcaccgg gcttataaaa tatgtggtca ataatcacaa tttttcaatg ggccattacc
      421 tggtgaaaag cataaacagt cattggagct ggggctatga aacaaattac agccagaata
      481 cattttccaa taacagggga agaatttttt tgcgtacagc ccttgagtat aatatctttc
      541 cttacaaaga agtgaatacc cgctcgttca cccttagtta tggtataaca gccaggcaaa
      601 accggtatta tgacactacc ttgtacaata aattaaaaga aaacttatac ggtcatggtt
      661 tcaatgcaca catgacttac aaccaaaaat ggggaacctc ctatattggg gtcaattatc
      721 ataattattt tcacgactgg aaatatttta acctgggtat ggaggcctat accagtgtac
      781 gggtaaccgg tgggctctca ttttatattt cagcatttgg cggattgtca agagatcagt
      841 tttacctgca aaaagggagt gctacttcac aggaggtgct cgcccgccag cggcaactgg
      901 catccggata ttcttattat ttcaacctgg gaatcaatta ccgtttcggt tcaaagctga
      961 ataattttgt aaacccgcgt tttgatggta ccagcaatag caactgaccg gttgaattac
     1021 ttttttaaag ctgcagcata tacttccagg catcttttcc tggccacttc atgcaccact
     1081 acaggtcgta cataagtgag ttcttcaaac tcaggcaccc actggcggat atactttagc
     1141 tcaggatcaa atttctgtgt ttgcagatag ggattaaata ctctgaaata aggagctgca
     1201 tcgcaaccac tgcctgccgc ccattgccag ccaccattat tggcagacaa gtcaaagtcg
     1261 agcagtttcc gggcaaaata gccttctccc catcgccagt cgatcagtaa atgtttggtg
     1321 aggaaagaag ctacgatcat cctgacccgg ttatgcataa agccggtggc attgagttcc
     1381 cgcataccgg cgtctacaat cggataaccg gtctgcccct tacaccaggc gtcaaactcc
     1441 gtttccttgt ttctccagcg gattttatca taatccggtt taaaggaatg accaacaacc
     1501 tgggggaaat gccataaaat catatgatag aaatcccgcc agatcagttc atttaagaat
     1561 gtttcattca acacgccggc ttcccgggcc agttcacgga tactgatggt accaaaacga
     1621 aggtgcacac ttaatcgcga ggtacccggc aaggaaggaa tatccctttg ttctttatat
     1681 tgggtgatga cttctttctt ccagcttttg gccgggaaag gaagacctgc cgctgtaaaa
     1741 cccattgcag ccagtgaagg aacagtcatc ggttgctggg acaggaaatg cttataaaat
     1801 ttctttgtat taaaaacctc aataggatga gtactgatca ccgctttcca tttccgtgaa
     1861 taaggagtaa aaacggtgta gggtttacca tcatccttca gcacttcatc tttttcaaaa
     1921 atcacatggt ccttgaagga atgaaattga atgtcctttg ttttcagcca ttgcgcgatg
     1981 gcctggtccc tttgccgggc atagggttca taatcatgat tggtgtaaac cgcttttatc
     2041 tcatactcat tcagcagatc ctggtacacc tgcaggggtt ttccgtaacg gacatccagg
     2101 gtactgccca tagtggtcag ttgctcctgc ataacagata ttgcccggtg aataaattcc
     2161 acgcgccggt catctttatc ttccagggag tcgaggattt ccgtatcaaa aataaaaagg
     2221 ggaagtacag gctggccgga ttgcagtgcc tggtacagac ccgcattatc gtgcaggcgc
     2281 agatcgcgtc ggaaccagaa aacagtcaca acaggtttca tgagggtgca acaagtttaa
     2341 cggggaatag tttgaatgaa acgggaataa agttccgaga catatttatt actgtaaata
     2401 ctatccctga aacggcgcac ccatttcacg ccatgtatcg cattgctgag aaaaacttca
     2461 ttggccgctt ccaatgcagc tatgttcacc ggcccttctt ccacggtata gccggcagcc
     2521 cgcatttccc ggaccaggta ttttcgcatt accccatcca cagctccttg ttccggtccg
     2581 ggagtaataa atttcccttc caggtataca aatagattgg caattgtact gtctgcaata
     2641 tgcccctcct ggttcagcag caggcaatca ttcagtttgt tttcctttgc ataaatggac
     2701 gccagggtat acggcaggaa gttggccgat ttcaatccgg agaaaagact aatctgcttg
     2761 ggagcatccg gatagatccc gatttccagc ccgttttcat tcaaccggct aaaactctcg
     2821 ggtagtggcc agcattcgat caggtattgc aatgtgcggt cggcatcata cagtccgcca
     2881 ttgccccgga aaaccgataa acgaacccgg cccagatccc gcacctggtt tttctccgcc
     2941 aattgtataa tctctgcagc gagcttactg gttgaaaaaa gtttgggccg gtcaaatttc
     3001 aaaagttcaa ggccagtaag gagccgttga aagtgcaact cctccaggag tatctttccc
     3061 tggtccagtt tcatcgtttc aaataagcca tccccatagc gataactccg gttggaagcc
     3121 agtagcaccg gttgatcagc cggggtgatt ttcccattga atgaaatgta catggctcaa
     3181 agtaacaaaa aagccatttg cgtttggcaa atggctttgt caa
//
LOCUS       DAOPMW010000391         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36084_length_3221_cov_4.969046, whole genome shotgun sequence.
ACCESSION   DAOPMW010000391 DAOPMW010000000
VERSION     DAOPMW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36084_length_3221_cov_4.969046"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLU11_14050"
     CDS             <1..1190
                     /locus_tag="PLU11_14050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPN60304.1"
                     /translation="ARIFIDNHQAPLAEQSLHEALVIRKKINDPFYIVYDMSSLAAYY
                     AANNQPQKGIVLGKEGVALAMESRLTSQLMMIYQALADNYKAAGLNEAYGETLEKILR
                     LKDSFNSINSSKMLADLQANRAAVRQEKLISEQKLNLTLKNYWLTGTILFTLMAAAIG
                     ILLFRHYSRKKKMEMEMVLADEKRNAEEAIRKAEEKERVRIASDLHDNLGAYAASMVS
                     NLNYIRIQENDPISSAAFTELKNNSGSVISQLNDTIWVLRKESLTLTALSDRVKLFIS
                     RIGRSYPDTRLEVTEYVEQDQQLSAAQAFHLYRVVQEGVNNALKHSGASLIYVIIESN
                     NHWKISITDNGKGFDPGLLTNGGGGHGLHTMQERSRESGCTINWRPNSSGGTIVEIVS
                     TTN"
     gene            1230..1904
                     /locus_tag="PLU11_14055"
     CDS             1230..1904
                     /locus_tag="PLU11_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN60305.1"
                     /translation="MKFSIALTEDNSINRNTFFQKIQTMDQLKLVFVANNGKQCLEEL
                     KGLPESRLPQVIFMDLEMPEMNGIETIRVAKSLHPAIHFIVLTVFDEEEKIFEAIRAG
                     ASGYLLKHEPAQVLQEAVTNVMEYGGAPMSPAVARKALLLMSRANLPDMPEDKNTVPS
                     MITSREEEILEYMVKGWDAKRIGVELNISVLTVRKHIANVYEKLHVQSKAEVISMAHQ
                     QNWFRS"
     gene            complement(1907..2356)
                     /locus_tag="PLU11_14060"
     CDS             complement(1907..2356)
                     /locus_tag="PLU11_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60306.1"
                     /translation="MSINVRPAVKEDCPRLLELVRELALYEKAPDEVTVSMEHFVESG
                     FGEKPVWWAFVAEEGNMIYGFALYYIRYSTWKGQAMYLEDIVVTASERGKGIGKLLFD
                     RLIEEAREKGFKRMVWQVLKWNEPAINFYKKYKADFDGEWFNSSIYI"
     gene            complement(2373..>3221)
                     /locus_tag="PLU11_14065"
     CDS             complement(2373..>3221)
                     /locus_tag="PLU11_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN60307.1"
                     /translation="NAIDEIIRIGKTTGVPVQVSHIKLAMHNLWGKADSLLAIMDQAR
                     KEGVQITSDIYPYAFWHSTIRVLFPDRNFSNEKEADMILRDITLPEDIILAAYEINPA
                     YEGKTLAAIAALENKTPARMLVELIARIEAWEKSHNDECRESIMATSMAETDIIRLMQ
                     WPYANICSDGSSSGRHPRGHGAFPRVLAKYTREEKALTLAQAIYKMTGLAATNLAIEK
                     RGFLRTGYFADIVLFNPDTVKDLATVEEPHALSEGILQVWVNGQSVYLTKKHSGLFPG
                     QLIKRK"
BASE COUNT          874 a          744 c          791 g          812 t
ORIGIN      
        1 aggcgcggat ctttattgat aaccaccagg caccgctggc tgaacaatca ttgcatgaag
       61 cactggtgat ccgaaagaag attaatgacc ctttttacat tgtctatgat atgagcagcc
      121 tggccgctta ctatgctgcc aataaccaac cacaaaaagg aatagttctc gggaaggaag
      181 gggtggctct ggccatggaa tccaggctta cgtctcaatt gatgatgatc taccaggcac
      241 tggctgataa ttacaaagca gccgggttaa atgaggcgta tggagaaaca ctggaaaaaa
      301 tccttcggtt aaaagactca tttaacagca tcaactcttc caagatgctc gctgacctcc
      361 aggccaaccg cgcagccgtg cggcaggaaa aactgatcag tgaacaaaag ctcaacctga
      421 cgctgaagaa ttactggctg acaggcacta tcctttttac attaatggcg gcggccatcg
      481 gaatattgtt gttccgtcat tacagccgga agaaaaaaat ggaaatggaa atggtattgg
      541 cggacgagaa acgaaatgcg gaagaggcca tccgcaaagc cgaagaaaag gaacgggtac
      601 ggattgcatc agatcttcat gacaatctcg gtgcttatgc ggcatccatg gtctccaacc
      661 tgaattatat ccgtatacag gagaatgacc ccatatcctc ggctgcgttt accgaattaa
      721 aaaataattc cggttctgtc atatcccagt tgaatgatac tatctgggta ttgcgtaagg
      781 aaagtcttac gcttactgca ctcagtgacc gggtaaaatt atttatcagc cggatcggaa
      841 gaagttaccc ggatacccgg ctggaagtga ccgagtatgt tgagcaggat cagcagttgt
      901 ctgccgccca ggcttttcat ttataccggg tggtgcagga aggggtgaac aatgcgctga
      961 aacacagtgg agcctcctta atctatgtta ttattgaaag taacaaccac tggaaaatca
     1021 gtattacaga taatgggaag gggtttgatc ccggattgct gacaaatggg ggaggtggcc
     1081 acggattaca taccatgcag gaaagaagcc gggagtcggg ctgcacgata aactggcggc
     1141 ccaactcatc tggcggaaca attgtagaaa tagtatctac tacaaactga ttattttatt
     1201 ccccggggag cccgggtaaa ttgcaggata tgaaattcag tatcgcatta acagaagaca
     1261 atagtattaa ccggaatacc ttttttcaga agatccagac aatggatcaa ctgaaactgg
     1321 tctttgtggc gaataacggg aagcaatgcc tggaagaact gaaaggctta ccggagtccc
     1381 ggctgccgca ggtgattttt atggatctgg aaatgcctga aatgaacggg attgaaacaa
     1441 tcagggtcgc caaatcactg catcccgcta ttcattttat cgtgctgact gtttttgatg
     1501 aagaggaaaa aatattcgaa gccatcaggg ccggtgcttc cggttatctg ctgaaacatg
     1561 aacccgccca ggtgctccag gaagcagtga ctaatgtgat ggaatacgga ggagcaccca
     1621 tgagtccggc ggttgcccgg aaagcccttc ttttaatgag cagggctaat ctgccggata
     1681 tgccggagga taaaaacacg gtaccttcta tgatcaccag ccgggaagaa gagatactgg
     1741 aatatatggt caaaggatgg gatgcaaaac ggatcggggt ggaactgaat atcagtgtac
     1801 tcaccgttcg taaacatatt gccaatgtat acgaaaaact gcatgtgcag tccaaagctg
     1861 aagtcatcag tatggctcat cagcagaact ggttcaggtc ataaactcat atataaatgg
     1921 aactgttgaa ccattccccg tcaaagtcag ctttatattt tttataaaaa tttatagccg
     1981 gttcattcca tttcagcacc tgccaaacca tccgcttgaa ccccttttct ctggcttctt
     2041 cgatcagccg gtcaaacaac agtttgccaa tccctttacc tcgttctgat gctgttacca
     2101 caatgtcttc caggtacatg gcctggccct tccaggtgga atagcggata taataaaggg
     2161 caaagccata aatcatgttg ccttcttccg cgacaaatgc ccaccaaacc ggcttttcgc
     2221 caaaaccact ttccacaaag tgctccatgg acacagttac ttcatccggt gctttttcat
     2281 acaaggccag ttccctgacc agttcgagca gccggggaca atcttccttc acggcaggtc
     2341 tgacattgat tgacataacg tatttcagtt ttttattttc gtttgatcag ctgccccggg
     2401 aagaggccgc tgtgtttttt agtaagataa acggattgtc cgttcaccca tacctgcagg
     2461 ataccttcag ataatgcgtg gggttcttcc acggtggcga gatctttcac ggtgtccgga
     2521 ttaaaaagga caatatccgc aaaatagccg gtgcgcagaa agcctctttt ttcaatcgcc
     2581 aggttggtgg cagccagacc ggtcattttg taaatggcct gtgcgagggt gagcgctttt
     2641 tcttcccgcg tgtacttggc taaaacccgg ggaaaggcgc cgtgcccgcg cggatgtctg
     2701 cccgaagagg atccgtctga gcagatattg gcatagggcc attgcatcag ccggataata
     2761 tctgtttcag ccattgaagt ggccatgata ctttcacgac attcatcatt atggcttttt
     2821 tcccaggctt caatccgcgc gatcagttca accagcatcc gggccggggt tttattttca
     2881 agcgcggcga tggcggccag tgtttttcct tcataggccg ggttgatctc ataagccgct
     2941 aggatgatgt cttccggaag ggtgatatcc cggagaatca tgtccgcttc tttttcattg
     3001 ctgaaattgc ggtcgggaaa caaaacccgg atcgtggaat gccagaaggc gtagggataa
     3061 atatcactgg taatttgcac gccttctttc cgggcctgat ccatgatcgc cagcaggctg
     3121 tctgcttttc cccagagatt atgcatggcc agtttgatat gactgacctg taccggaacc
     3181 ccggtagttt tgccgatccg gatgatttca tcaatggcat t
//
LOCUS       DAOPMW010000392         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36221_length_3212_cov_5.946785, whole genome shotgun sequence.
ACCESSION   DAOPMW010000392 DAOPMW010000000
VERSION     DAOPMW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36221_length_3212_cov_5.946785"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="PLU11_14070"
     CDS             complement(<1..1570)
                     /locus_tag="PLU11_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN60308.1"
                     /translation="MWKNIFMKSAATMLLLVIGLTVSAQVVIRGKIADKKGNPLQGVS
                     VSVMDADNRIVSGTSTDIEGNFVLKNVNTKYKLSFSFIGYKTQTIPVGTKTEFAILME
                     DSQTDLGEVVIMSRPTSSNGMVNIQEKNLTTAVARISAKDLEEMQSASIDQALQGRLS
                     GVDITASSGDPGAAMNIRIRGVSSINTTGIPLIVLDGMPLETEIPSDFNFGTADEQGY
                     AQLLNISPADIKEITVLKDAAATGIWGSRGANGVLIITTKRGVIGKPSINYTFKGSVS
                     LVPAAIPLLNGDQYSTLIPEAVMNRTGTPLNTTQVREFNYDPNDPYWYNNYSNNTNWV
                     DAVSRTGNLQDHTVSLTGGGEKARYFASIGLFDQTGITLGTSLQRLNTRINLDYNVSE
                     RIKIFTSVSFTHTDQDRNYLGGNSASGEASIRGMAYIKMPNMSVFEYDELGNLTTNYF
                     SPAANVQGAYTRIYNPVAMAAKAKYNVLGERIVPRFMVDYFIVRNVLKATFDIQFDIN
                     NTKNKSFLPQIATGR"
     gene            complement(1594..>3212)
                     /locus_tag="PLU11_14075"
     CDS             complement(1594..>3212)
                     /locus_tag="PLU11_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPN60309.1"
                     /translation="YTGFNVAEAKVVMKDIAAENGVIHVVDRVITSLPSLDQYIGSNP
                     NYSEFKKILDKYLVQYVLNATVTDNYKTVTGNAVDVFTKVYNASLAFSPNNENFLKLQ
                     DNDGQSDTYTMFVPDNATLTAYINSVLLEHYTSLDAMPVSVIYDFVNAHMWRTAVWPS
                     KFASTYNSVGEEARFNPASNVVDKKVLSNGIFYGTNKVQEANVFSSVYGKAYLDPNYS
                     IMTSLLNLDLKFQISNVSQRYTLFLISNAAFNAAGYFADPTVSNNVNEQWRYTPPGGG
                     TAITGSSALVRLQRILNMHVIPGRDLTSLVNPGVAMSYSGEFIKFSNNTVAASGNSDA
                     AQVANVTNTKTAKNGTVYYLDKILQFSELTPGRHIETLGGTSAATSQFYNFFQYLKSS
                     AIWNNTTKEIVAVASGSFYTFFIPNNAAIVAAVNAGYLPGTGTPPNMVPTFNPSLPAD
                     KDKVINFLYYHILNKRNIATDGQESGSIETLYKFPNGDPSTIFVNNNTANNLIVSDMM
                     NRTANVIFASSNNLSNRASIHLIDNYLRKN"
BASE COUNT          805 a          745 c          760 g          902 t
ORIGIN      
        1 gtcttccggt agcgatctgc ggcaggaagc ttttgttttt agtattgttg atatcgaact
       61 ggatatcaaa agtggctttc agcacattgc ggacaataaa gtagtccacc atgaaacggg
      121 gtacgatcct ttcacccagc acattgtatt tagccttggc ggccatcgcc accggattgt
      181 agatccgggt gtaagcgccc tgtacgttgg cagcgggtga aaaataatta gtagtcaggt
      241 tacccagttc atcgtattca aacacactca tattgggcat cttaatatac gccataccgc
      301 ggatggaggc ttccccgctg gccgagttac cgcctaaata gtttctgtcc tgatcggtat
      361 gcgtaaagga tacactggta aagattttga ttctttcaga cacgttataa tccaggttga
      421 tccttgtatt taaacgctgc agtgaagtac ccagtgtaat accggtctga tcaaaaagtc
      481 cgatagaagc aaagtacctg gccttttcac cgccaccggt cagactcacg gtatgatcct
      541 gcaggttacc ggtacgggat acagcgtcga cccagttggt attgttgctg taattattat
      601 accagtaagg atcattggga tcatagttaa attcacgcac ttgtgtggta ttgagcggcg
      661 taccggtacg gttcatcacc gcttccggaa tcagggtgga atactgatct ccattcaata
      721 aaggaatggc agccggtacg agtgatactg agcctttgaa tgtatagtta atggaaggtt
      781 taccaatcac cccgcgtttg gtggtgatga tcagtacccc gttggcaccc cgtgaacccc
      841 agatacccgt ggcagcggcg tctttcagta cggtgatttc cttgatatcg gccggggaga
      901 tatttaacaa ctgggcatat ccttgttcat cagctgttcc gaagttaaag tcactgggga
      961 tctcggtttc cagcggcatt ccatccagaa cgatcagggg aataccggtg gtgttgatgg
     1021 aagacacccc gcggatccgg atattcatgg cggcacccgg gtcaccgctg ctggccgtga
     1081 tatccacgcc actcagccta ccctgcaggg cctggtcgat actggcggat tgcatttctt
     1141 ccaggtcttt ggcactgatc ctggctaccg ccgtggtcag gtttttctcc tggatattga
     1201 ccattccatt ggaggaagtg ggtcgggaca tgatcactac ttctcccagg tcggtctgcg
     1261 agtcttccat caagatggca aactctgttt tagtaccaac aggaatggtt tgtgttttat
     1321 aaccgataaa ggaaaaagag agtttatatt ttgtatttac atttttcaat acaaaatttc
     1381 cctcgatatc ggtggaggta ccactgacaa tccggttatc cgcatccatc acggaaacag
     1441 ataccccctg caaggggttt ccctttttat ctgcgatctt acccctgatt accacctgtg
     1501 ccgacacggt cagtccgatg accagcagaa gcattgtagc cgcagatttc atgaatatat
     1561 tcttccacat acagaatgag tttggatgga tgattaattt ttccgtaaat agttatcgat
     1621 caggtgaatg ctggcccggt tgctcaggtt attactgctg gcaaaaatga cattggctgt
     1681 ccggttcatc atatcactga caattaaatt attggctgtg ttattattga caaagatcgt
     1741 ggacgggtct ccgttcggga atttgtacag cgtctcaata cttccgcttt cctggccatc
     1801 tgtggcgata ttccgtttat tcagaatatg gtagtacaga aaattgatca ctttgtcttt
     1861 atccgcaggt aaggaaggat taaaggtggg taccatattc ggaggggttc ctgttcccgg
     1921 taaataacca gcgtttacag ccgcaacgat cgccgcgtta ttggggataa agaaggtgta
     1981 gaatgaccca gaagccacag ccacaatttc cttggttgta ttattccaga tggctgaact
     2041 tttcaggtac tggaagaaat tatagaactg tgaggttgcg gccgaagttc ccccaagtgt
     2101 ttcaatatga cgccccggag tgagttcact aaactggaga attttatcca ggtaatacac
     2161 ggtaccgttc tttgcggtct tggtattggt cacattggct acctgtgccg catcgctgtt
     2221 tccggatgca gcaactgtat tattggaaaa cttaataaac tcaccggaat aagacatcgc
     2281 cacacccgga ttcaccaggc tggttaaatc tcttcccgga atcacatgca tattcagaat
     2341 ccgttgcagg cgtaccaagg cggaagaccc tgttatggcg gttcctcctc cgggaggggt
     2401 ataacgccac tgctcattta cattattact tacggttgga tcagcaaaat atccggccgc
     2461 attaaatgcc gcgttgctga tcaggaagag ggtatatctc tggcttacat tagatatctg
     2521 gaatttgagg tccaggttca gcaggctcgt cataatggaa tagttcggat ccaggtaggc
     2581 tttgccgtat acactgctga acacattcgc ctcctgtacc ttattcgtac catagaaaat
     2641 accgttgctg agtacttttt tatcgaccac attggatgcc ggattaaaac gggcttcttc
     2701 tcctactgaa ttataagtag aggcaaattt agacggccat acagcggtcc gccacatatg
     2761 cgcatttaca aaatcataaa tcacactgac cggcatcgca tccaggctgg tataatgttc
     2821 cagcaatacg gagtttatat aggcggtcag ggttgcatta tcgggaacga acatggtata
     2881 tgtatccgat tgtccgtcat tatcctgcag cttcaggaaa ttctcattat tgggggagaa
     2941 tgccaggctg gcgttataca ctttggtaaa tacatcgacg gcatttccgg tcaccgtttt
     3001 ataattatcg gtaacggtgg cattaagaac atactgcacc aggtatttat ccagtatttt
     3061 tttaaactca ctgtagttcg gattgctgcc gatatactga tccaggctgg gcaatgacgt
     3121 gatcacccgg tccacgacat gaatcacgcc gttctcagcc gcaatatcct tcatcaccac
     3181 tttcgcttcc gccacattaa aaccagtata gg
//
LOCUS       DAOPMW010000393         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36239_length_3211_cov_4.673638, whole genome shotgun sequence.
ACCESSION   DAOPMW010000393 DAOPMW010000000
VERSION     DAOPMW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36239_length_3211_cov_4.673638"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLU11_14080"
     CDS             complement(<1..809)
                     /locus_tag="PLU11_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60310.1"
                     /translation="MKFFTTLNKLLNVDSIPADQQNSRENFLVREGNQVHKALIYIEA
                     FYGFFFIFVTWYLGYYYQMALNAALFVFSCLAYIIQDKGFAFYSKIFNLSQLILIIGL
                     MYYFPASPYGIHINDSVLVFYIPVSVGTLIAFQGKENKYGYLLSGIILLVALFLIITD
                     FHYMSDHQAPLLSSVSYDLLFNIIGAAVATFVEVAYILALNNRLNESLSKANHELDNF
                     VYIVSHDLRSPLLLTKGLLDISKLKINEQKEVLKYLDLAGKSINNLDDIIK"
     gene            complement(1012..1713)
                     /locus_tag="PLU11_14085"
     CDS             complement(1012..1713)
                     /locus_tag="PLU11_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPN60311.1"
                     /translation="MPFSYNFGEFPGQTINQGPKLINFSEYKGEADIELCCTQLDNTT
                     AKEQAKIVDEWCDHLSNNTLPLKSIWMETRISQKIFDAICKQTSLTSLWIKWGVYPDL
                     SAISQLTNLEHLHLGNGTSITDLSPVGRLPQLKTLESDGLVNVHDYRFLSSLTALQDL
                     DIEGDGFSSMKPVLLSDLKFLESIPWLQRLCLKMVRVKDKDYSPILKIKDLKWLSLPH
                     YKEVQQQEHLFKNLY"
     gene            complement(1905..2546)
                     /locus_tag="PLU11_14090"
     CDS             complement(1905..2546)
                     /locus_tag="PLU11_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60312.1"
                     /translation="MKKYYFTIMGWLLVFLSVYAFSDNLFTDVGQKSNKDPKYIIHGL
                     FCFAWFIIFLIQVNYVRKLQYKQHIRLGLAGMLAAIGVFVTTLYIFIVIFKGWQLMSP
                     EVKANRFFMPAYAVFVYLGYKYRKQPAAHKRFIFIATIYMLGPVLGRAMAHSFLDNLL
                     VSDLSWDITQMSIWTAFIISLLIYDRMILKRIHPVTIAGTGYYTLTWVLAWIT"
     gene            complement(2682..3167)
                     /locus_tag="PLU11_14095"
     CDS             complement(2682..3167)
                     /locus_tag="PLU11_14095"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN60313.1"
                     /translation="MLKSYWLLITFLGVALSGGAQLAKTDSLFLALKKQDSIFFERSF
                     NLCDLDYLDKTTHPELVFFHDQGGMQHKTEFLLAVKNNICGNPEQKPIRKAVTNSFEV
                     FPLFSNGIFYGVIQTGIHEFYIRETGKPDRYSSRAKFTHVWLLDNGRWLLREVLSYDH
                     K"
BASE COUNT          879 a          663 c          800 g          869 t
ORIGIN      
        1 tccttgatga tatcatccag gttgttgatg cttttaccgg cgaggtccag gtatttaagc
       61 acttcttttt gttcgttaat tttcagtttg gaaatatcca gcagaccttt ggtcagcagc
      121 agcggggagc gcaggtcatg gctcacgata taaacaaaat tatccagttc atgattggct
      181 ttggacaggc tttcgttcag ccggttattc agggccagga tataggctac ttcaacgaaa
      241 gtggccacgg ctgctcctat aatattaaag aggagatcat aacttacgct gctcaggagg
      301 ggtgcctgat gatcggacat gtaatggaaa tctgttatga tcaggaagag ggccaccagc
      361 agaataatcc ctgataaaag ataaccatac ttattctctt tgccctgaaa ggcaatcagt
      421 gtaccaacgc tcacggggat gtaaaacacc agcacactgt cgtttatatg aataccatag
      481 ggagaagcag gaaaatagta catcagtccg atgataagaa tgagctggct gagattaaaa
      541 attttagagt agaaagcaaa gcccttgtct tgtatgatat aggcgagaca ggaaaaaaca
      601 aataaagccg cgttcagcgc catttgatag taatagccca ggtaccaggt gacgaaaata
      661 aaaaagaagc cataaaaagc ctcaatataa atgagggctt tatggacctg gtttccttcc
      721 cgcaccagga agttttcccg gctgttctgc tgatcagccg gaatgctatc cacattcagc
      781 agcttgttaa gcgtggtaaa gaatttcata tgcgcctgat ggttgtaagt acatcatccg
      841 gcgctaaatg taaataccgg gaggaagaaa aactatgatt tgagtgaaac agggtactta
      901 atttcaagtt cgggtatcag gtgtcgggtg tcaggtgtcg ggggaacagc ccgtacttag
      961 aaagtaattg taaacagtac gtcagcgtac ttcgtatttc tgacttctta cttagtatag
     1021 gtttttgaat aaatgctcct gctgttgtac ttctttataa tgtggcaggc tcagccattt
     1081 caggtctttt attttaagga tgggggaata atccttgtct ttcacccgta ccattttcag
     1141 gcagagccgt tgcagccagg gaatggactc caggaatttc aggtcagaca atagaaccgg
     1201 cttcatggag gagaacccat ctccttcaat atcgagatcc tgcagggcgg tcagggaaga
     1261 gaggaaccgg tagtcgtgca cattgaccaa tccgtcggat tccagtgttt tcagttgagg
     1321 caaacggcct accggggata gatcagtgat acttgttcca ttgcccaggt gcaggtgttc
     1381 gaggttggtc agttgactaa tggccgacag gtctgggtac accccccatt tgatccagag
     1441 agaagtaaga ctggtctgtt tgcagattgc atcgaagatt ttctgcgaga tccgcgtttc
     1501 catccagatg cttttcagcg gcaaggtatt attagataga tgatcgcacc attcgtccac
     1561 gatctttgcc tgttccttcg cggttgtatt atccagttgg gtgcaacaaa gttctatatc
     1621 cgcttcgcct ttgtactcac tgaagttgat cagtttgggt ccctggttaa tggtttgtcc
     1681 gggaaattcg ccgaagttgt agctgaatgg cattgggtaa gggtagggag aaaaggaagc
     1741 cggtagttag aagtccccca tagcaggtgt tcgctgttag ctgataggtg tttgggaaca
     1801 gccccttctt aacaggtaga atctaaacca aaatacggcc tcttgcttta ggggatccca
     1861 ctcgtacttt gtcccgctac ttcgggattc tgacatcgta aatattatgt tatccaggcc
     1921 agcacccagg tcaatgtgta atacccggtt cctgcaattg taactggatg aatccgcttt
     1981 aatatcatgc ggtcatagat caggagggaa atgataaatg cggtccagat actcatttgg
     2041 gtgatatccc aggacaagtc gcttacgagc aggttgtcaa ggaaggagtg agccatggcc
     2101 cgtccgagaa caggtcccag catatagatg gtggcgataa atataaagcg tttatgagct
     2161 gccggttgtt tccggtactt gtaaccgaga tagacaaaca ccgcataggc cggcataaag
     2221 aaccggttgg cttttacttc gggtgacatc agttgccatc ctttaaaaat cacgataaaa
     2281 atatagaggg tagtaacaaa tacaccaata gctgccagca tccccgccag acccagccgg
     2341 atatgctgtt tatactgcag tttccttaca taattcacct ggatcagaaa gatgatgaac
     2401 caggcaaagc aaaaaagtcc atggatgatg tatttcgggt ccttgttgga cttctgcccg
     2461 acatcagtga aaagattatc cgagaaggcg tatactgata aaaataccag cagccagccc
     2521 attatggtga agtagtattt tttcaagtgt gtattgccgg gtgtgtactc taatataggc
     2581 aattttgtag gaatcggctg ttggatcttc tatatctcgc tgcttgcact ctgtagtgaa
     2641 acgaaggagt ggcgctcgaa ataagagtgg aggtgtcttt cctatttatg atcgtagctc
     2701 aaaacttcgc ggagcagcca gcggccattg tcaagcagcc agacatgggt gaattttgcc
     2761 cggctgctat agcggtcggg tttaccggtt tcccggatat aaaattcatg gatgccggtc
     2821 tggattacgc cgtagaaaat tccattgcta aaaagcggaa atacttcgaa actgttagtc
     2881 accgctttgc ggatgggctt ttgttcaggg ttgccgcaga tattattttt tactgcaagg
     2941 agaaactctg ttttgtgttg cataccaccc tggtcatgga aaaagaccag ttccggatga
     3001 gtagttttat cgagataatc caggtcgcat aagttaaagg accgttcgaa aaagatactg
     3061 tcctgttttt tgagggccag gaaaagggaa tcggtttttg ccaattgtgc acccccgctt
     3121 aacgcgaccc cgagaaatgt tattaaaagc cagtatgatt tcagcataaa caggattgag
     3181 catcaaagat gccccgggaa tgctgaatag c
//
LOCUS       DAOPMW010000394         3183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36723_length_3183_cov_6.720588, whole genome shotgun sequence.
ACCESSION   DAOPMW010000394 DAOPMW010000000
VERSION     DAOPMW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36723_length_3183_cov_6.720588"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLU11_14100"
     CDS             complement(<1..314)
                     /locus_tag="PLU11_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HPN60314.1"
                     /translation="MTNDLKTEEPKNESFRDRIATVDAKGKRNWVFAHKPKGRFYNIR
                     TIVSLGFFILFFALPFIKVNGRPFFLFNVPEAKFIIFGKVFWPQDFFIFGLTMVTFII
                     FIV"
     gene            complement(347..>1327)
                     /locus_tag="PLU11_14105"
     CDS             complement(347..>1327)
                     /locus_tag="PLU11_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPN60315.1"
                     /translation="DVKLKKKKKAANPATLASVITGLLLLAAPALFAQDATGDTANAV
                     TTGAKTIAGMPSSTFYILATVMFLELFIILALLINIKFLLKAEKDKITSETETEEEKA
                     NKLTWWDKFNALRPVTEESSLDLGHDYDGIRELNNRLPGWWLYGFYGCILFAGVYLYR
                     FHVSHTAPSSIQEYEQSVVRAESQLKEFIKKQGDAVDETNVKLLTGAEDLAAGKALFV
                     KSCAACHLESGAGIVGPNLTDDYWLHGNDIKSMFKVVRYGINAMPAWQTQMSNKQIAQ
                     VSSYIKTLHGTNPPNPKAPQGTLMKEEAAPAAPASPAADSAAQTKKVAMN"
     assembly_gap    1328..1427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1584..3131)
                     /gene="ccoO"
                     /locus_tag="PLU11_14110"
     CDS             complement(1584..3131)
                     /gene="ccoO"
                     /locus_tag="PLU11_14110"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HPN60316.1"
                     /translation="MQDALVQWWYGHNAVAFFLTTPILGLMYYFLPKAANRPVYSYRL
                     SIIHFWALIFIYIWAGPHHLLYTSLPDWAQSLGTVFSIMLIAPSWGGMLNGLFTLRGA
                     WDKVREEPVLKFMVVAITCYGMSTLEGPLLSLKNINAISHFTDWTIAHVHIGGLGWNG
                     FMAFAMIYYLVPKMWNTELYSKKLATNHFWLGTIGIILYAVPMYWAGFEQASMWKQFT
                     PEGQLKFQFLETVTNLIPMYVARSVGGLLYLIGVHMLVYNIYKTMAKGRFIAEEAAEA
                     PALVKEKPHKEYWHRILERKPVTMLVLSLIVVSIGGLVQMIPTFLVKSNVPTIASVKP
                     YTPLELQGRDIYIREGCYTCHSQMIRPFRDEVARYGEYSKAGEFVYDHPFQWGSKRTG
                     PDLARIGGKYPDSWHYNHMYDPRSMSPGSLMPQYVWLLDDALDTSSTPAKIRAMQTLG
                     VPYPEGYDQVANRDLMHQADSIAANLKKDKIETAPNAEIVALIAYLQRMGRDIKLEKK
                     PIAENIK"
BASE COUNT          848 a          692 c          696 g          847 t
ORIGIN      
        1 acaatgaaaa tgataaaggt gaccatcgta agcccgaaga tgaaaaagtc ctgtggccag
       61 aatactttac caaaaatgat aaattttgct tcgggtacat tgaacagaaa aaaaggcctg
      121 ccattcactt taatgaatgg cagggcaaaa aagagtataa agaagcctaa gctgacaatg
      181 gtcctgatat tgtaaaaacg accttttggt ttatgggcaa atacccagtt gcgtttgcct
      241 ttggcatcca ccgtggctat ccggtcacga aacgattcat ttttcggttc ttcagtctta
      301 agatcattag tcattgctta cactcctttt attacgcaca ttattttcag ttcatggcta
      361 ctttcttcgt ttgcgcagct gaatccgctg ccgggcttgc cggtgctgcc ggggctgctt
      421 cttctttcat caacgtaccc tgaggtgctt ttggattagg aggattggtt ccgtgtaaag
      481 ttttaatata gcttgatacc tgggcaattt gtttgttgct catttgtgtc tgccatgcag
      541 gcatggcgtt tatgccatat cgtacaactt taaacattga cttgatgtca tttccatgta
      601 accagtaatc atctgtcagg tttggaccaa caatgccggc gccggattcc agatggcagg
      661 cggcgcaaga cttgacaaat aatgcttttc ccgctgccag atcctcagca ccagtcaata
      721 atttgacatt agtttcatca accgcatctc cttgcttttt tatgaattct tttaattgag
      781 actcggcccg gacaacagat tgttcatatt cctgaatgct ggaaggagca gtatgggaaa
      841 catggaaacg atataaatat acgccggcaa ataagataca tccataaaag ccatatagcc
      901 accagccggg taaccggtta ttcagttcgc ggataccgtc ataatcgtgg cccagatcaa
      961 gggaagattc ttcagtaacg gggcgaaggg cattgaattt atcccaccag gtcagtttgt
     1021 ttgctttttc ttcctcagtt tctgtttctg atgtgatctt atctttctct gctttcagga
     1081 ggaacttaat attgatcagg agagccagaa tgataaacag ttctagaaac attaccgttg
     1141 caagtatata gaaggtagag gagggcatgc ccgcgatggt ctttgcacca gtagtgaccg
     1201 catttgcagt gtcgccggtt gcatcctgcg caaaaagagc aggagcggcc agtaacagca
     1261 atccggtgat gacagaagct aaagtagccg ggttagccgc ttttttcttc tttttcaatt
     1321 ttacatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga atccggctga
     1441 tctcatcata gcttttcttc ttggttttaa atacccaggt cagcatgagg gtaaaaaaca
     1501 ggaagaataa cagcagggaa gagagaccga aaatgtccac accgcttact ttttctagat
     1561 agttgatgaa tttcatttct gaattattta atattttcag cgattggttt tttctccagt
     1621 ttgatatcac gtcccattcg ttgcagataa gcgatcaatg caacaatctc agcatttggc
     1681 gccgtttcaa tcttgtcttt tttcagatta gctgcaatgg aatcggcctg gtgcatcagg
     1741 tcacgattag ctacctggtc atatccttct ggataaggca cacccagtgt ctgcatggcc
     1801 ctgatcttgg ccggtgtgga gcttgtatcc agcgcatcat ccagcaacca tacgtattgt
     1861 ggcatcagtg atccaggaga cattgaacgg ggatcgtaca tgtgattata gtgccagctg
     1921 tcaggatatt taccgccgat acgggccaga tccggaccag tacgcttgct gccccattgg
     1981 aacgggtggt catagacaaa ctcaccggct ttggagtatt ccccataacg ggctacttca
     2041 tctctgaacg gacggatcat ttgcgagtga caggtataac aaccttcccg gatatagata
     2101 tcacgtccct gtaactccag cggcgtataa ggtttaacgc tggcaatagt tggtacattg
     2161 gattttacca ggaaagtggg tatcatttgt acaagacctc caattgatac tacaatcagg
     2221 ctcaatacca gcatggtaac aggcttgcgt tccaggatac ggtgccagta ttccttatgt
     2281 ggtttttctt taacaagggc aggggcttcc gcagcttctt cagctataaa ccggcctttt
     2341 gccatcgttt tataaatatt atagacaagc atatggacac caataaggta taaaagacct
     2401 cctacactac gggctacata catgggtatc aggttggtca ccgtttcaag aaactgaaac
     2461 ttaagctgac cttccggggt aaactgtttc cacatagatg cctgttcaaa accggcccag
     2521 tacatgggta ctgcatataa aattataccg atggttccca gccagaagtg gttggtggcc
     2581 agttttttgg agtagagttc tgtattccac attttcggaa ccagataata gatcatggca
     2641 aaggccataa aaccattcca tccaagaccg ccaatatgca cgtgggcaat agtccagtcg
     2701 gtgaagtgcg agatcgcatt gatatttttc agactcagca gcgggccttc cagggtactc
     2761 ataccataac aggtaatggc cactaccata aatttcaata ctggttcttc ccttacctta
     2821 tcccaggcac cacgtagggt gaacaaacca ttcagcatac ctccccagct cggcgcaatc
     2881 agcatgatgg agaataccgt acccagggat tgagcccagt ctggtagtga ggtatataac
     2941 aggtggtgag gaccggccca gatataaata aaaatcagcg cccagaagtg aatgatggaa
     3001 agacgataag aataaacggg tcgattagcc gctttgggca gaaaataata catcagaccc
     3061 agaataggag tagtcaggaa gaaagccacc gcattatgtc cataccacca ttgtaccaag
     3121 gcatcctgca caccggctta ccaactgtag cttttggtaa gagaaaccgg aatagcaatg
     3181 gag
//
LOCUS       DAOPMW010000395         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36862_length_3175_cov_6.359615, whole genome shotgun sequence.
ACCESSION   DAOPMW010000395 DAOPMW010000000
VERSION     DAOPMW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36862_length_3175_cov_6.359615"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLU11_14115"
     CDS             <1..1018
                     /locus_tag="PLU11_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN60317.1"
                     /translation="LNAQVFTGTAKYNECIAACDSVMNAGGGTQYALESRATYFNQFG
                     PTNGPAYKEFVFAIPFDPSTSNGYMFYSRYDLNRNLGIKYRYSGSTPGTNVDPVLNQT
                     TGNGLVNNKPSGPRCTTQEFYAYFNDVNDIRNQQWLGGLQYWANGNPILVRTTNLGYD
                     QFYSGGSPGATLTYHLNISPLTTSRLGASSYDVGRDELAWNTGYRNIKFLADPNSITR
                     NQNNDVPVFRLSDIILMKAEAIQRGGTATNGHTALSLVNTLRAVRTTSAALTSLTIDD
                     IYAERTRELSWEGWHRNDMIRFGRFEDSWGLGKTNTDTYRRVFPIPTSAIATNPNLAQ
                     NTGY"
     gene            1097..2086
                     /locus_tag="PLU11_14120"
     CDS             1097..2086
                     /locus_tag="PLU11_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN60318.1"
                     /translation="MNRNYYWLVIFSGMLLHAVAGNTQVLINDPAYTRGDTLPVRKDS
                     VLNFIVMGDWGRNGADHQKEVARQMDITATVYHTQFIISTGDNFYPSGVISEWDPLWR
                     YSFEDIYTGFHLQWDWYPVLGNHDYKSNPDAQVKYSKISRRWQMPARYYAKKFYINGD
                     TSQAVLIAFIDTNPLIPLFYKNREYGPNVRSQDSTKQKKWLEKVLSDPSPAIKWKIVV
                     GHHPMYSGGGRTETYDTKAIRKWLSPVFAKYGVDVYLSGHEHSLQYMGPVDKTHHFIS
                     GAASEKTPARLIDISRFSASEYGFMLFSIQKNQLSMQTIDYTGKTIYQTTLSR"
     gene            2093..>3175
                     /locus_tag="PLU11_14125"
     CDS             2093..>3175
                     /locus_tag="PLU11_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60319.1"
                     /translation="MPTVELNKRSSISTGRLHSLDALRGFDMWWIVCGEGIFHGLSGA
                     IKDKYGLVQSNVDWQIRGEAPMNLLERSLIGISNQLHHSVWNGFTFYDLIFPLFIFIA
                     GVSMPYSMGKQLDKAGTDKTAARQAILHSLFKRTIVLLLLGMLVNGALQLNGYEKTRF
                     ASVLGRIALGCLGGALIYLYVPGKKIVYWITGILLAYWALLYLIPVPGYGAGVITPEG
                     NITAWFDRNFLPGKLHRKVYDPEGLLSSIPAVASALLGVLCGQYIRSSAQKGFSAGQA
                     LYLFGTGLVLLLSGWLWNLVFPVNKILWSSSYVLVAGGWSMMVLALFYYIIDVKGFRR
                     WSLPLVWIGTNSILIYVAAHGLVNFES"
BASE COUNT          797 a          772 c          772 g          834 t
ORIGIN      
        1 cctgaatgca caggtgttta cgggaacagc aaaatacaat gaatgtatcg ctgcctgtga
       61 cagtgtgatg aatgccggcg gcggaacaca gtatgcattg gaaagccgtg ccacttattt
      121 taatcagttt ggtccaacga atggccctgc ctataaggag tttgtctttg ctattccctt
      181 tgacccctcc acatccaacg ggtatatgtt ttactcccgg tatgacctga accggaacct
      241 gggtatcaag taccgttatt ctggttccac acccggtacc aatgtagatc cggtgcttaa
      301 tcagaccacg ggtaacggat tggtgaataa taaacccagc ggaccccgtt gtaccaccca
      361 ggaattctat gcctatttca atgatgtcaa cgatatccgt aaccagcaat ggttgggtgg
      421 attgcaatac tgggccaatg gtaatccgat cctggtgcgt accaccaatc tcggatatga
      481 tcagttctac agtggtggca gtcccggtgc cacgttgact tatcacctga acatttctcc
      541 gttgaccaca tcaagactgg gtgccagttc ttatgatgtg ggacgtgatg agctggcctg
      601 gaatacaggt taccggaata taaaattcct ggccgacccc aattctatca cccggaatca
      661 gaacaatgac gtgcccgtgt tccgtttatc tgatattatc ctgatgaaag cggaagcgat
      721 ccagcgcggc ggtaccgcca caaatggtca tacggcctta tcattggtaa ataccctgcg
      781 tgccgtacgt accacttcag cagcactcac ctcccttaca attgacgata tctatgcaga
      841 gcgtacccgt gaactttcct gggaaggatg gcaccgtaat gatatgatcc gcttcggccg
      901 tttcgaagac agctggggac tgggcaaaac aaacacagac acttaccgtc gtgtgttccc
      961 gatcccgaca tcagcgatcg ctacgaaccc gaacctggcg caaaataccg gctattaagg
     1021 ttaagcagta cctcagtagc aggcaatcgg taacccggag ccgtaaggct ccgggttttt
     1081 taatgtacat ttcagcatga acagaaacta ctactggctg gtgatcttct ccggtatgtt
     1141 attgcatgca gtggcaggca atacccaggt gctgatcaat gatccggcct atacacgtgg
     1201 tgatacactc ccggtccgga aagattctgt actgaatttt atcgttatgg gtgactgggg
     1261 ccgcaacggg gcggatcatc agaaagaagt agcccggcaa atggacatca ccgctaccgt
     1321 ttatcatacc cagttcatca tttccaccgg tgataatttc tacccgagtg gcgtgatcag
     1381 cgaatgggat ccgctttggc gctattcctt tgaagatatt tataccggct tccacctcca
     1441 gtgggactgg tacccggtcc tgggaaatca tgattacaaa agcaatccgg atgcacaggt
     1501 aaagtatagc aaaatcagcc ggcgctggca gatgcccgcc cgttactatg caaagaaatt
     1561 ttatatcaat ggggatacgt cgcaggcggt actgatcgct tttattgata ccaatccatt
     1621 gatccccttg ttttataaaa acagggagta cggtcccaat gtccgcagcc aggatagtac
     1681 caaacaaaaa aaatggctcg agaaagtctt aagcgatcct tcgcccgcta tcaaatggaa
     1741 gattgtggta ggccatcatc cgatgtattc aggcggcgga cgtaccgaaa cctatgatac
     1801 aaaagccatc cggaaatggc tgagcccggt ctttgctaaa tacggcgtgg atgtatacct
     1861 cagcggacat gagcatagtc tgcagtatat ggggccggtg gacaagaccc atcattttat
     1921 ttcaggggca gcttctgaaa aaacaccagc ccgcctcatc gatatcagca ggttttctgc
     1981 atccgaatac gggttcatgc ttttttccat tcagaaaaac caactttcca tgcaaaccat
     2041 cgactatacc ggtaaaacca tttaccagac gaccctgtct cgctaattca ttatgccgac
     2101 cgttgaatta aataaaaggt cctccatctc cacaggacgc ctgcattcac tggatgcact
     2161 gcgtgggttt gatatgtggt ggatcgtttg tggtgaaggg atctttcacg gactgtctgg
     2221 agcgatcaag gataagtacg gtcttgtgca aagcaacgtt gactggcaga tccgcggcga
     2281 agccccgatg aacctgttgg aacgtagcct tattggtatc agcaatcaat tgcatcattc
     2341 cgtatggaac gggtttactt tctacgatct gatttttcca ctgtttattt ttatagccgg
     2401 agtcagtatg ccttattcca tgggcaaaca actcgataaa gccggtacgg ataagaccgc
     2461 tgcacgtcag gctattctcc attcactgtt caaaagaacg atcgtcctgc tcttactggg
     2521 tatgttagtg aacggggcac tccaattaaa tggatacgaa aaaacccgat ttgccagtgt
     2581 actcggacgt attgcattgg gttgcttagg aggcgcattg atttatctct atgtaccggg
     2641 taaaaaaatt gtatactgga tcaccggtat cctgctggcc tattgggccc tgttgtatct
     2701 cattccggta cccggctatg gtgccggggt cattactccg gaaggaaata ttaccgcctg
     2761 gtttgaccgg aactttctcc cgggcaaatt gcaccggaaa gtatatgacc ccgaagggtt
     2821 actcagcagc atcccggccg tggcaagcgc cctgctgggt gttctttgcg ggcaatacat
     2881 ccgttcatct gcacaaaagg gcttttcagc gggacaggca ctgtatctct ttggtacagg
     2941 attagtttta ttactcagtg gctggttatg gaatcttgtt tttccggtca ataaaatcct
     3001 gtggagctcc tcttatgtgc tggtggccgg tggatggagc atgatggtat tggctctctt
     3061 ttattatatt attgatgtga aggggttccg gagatggagc ttaccattgg tctggattgg
     3121 aacgaattcc atcctgatct atgtagccgc acacggcctg gtcaattttg aatcc
//
LOCUS       DAOPMW010000396         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36977_length_3167_cov_7.195051, whole genome shotgun sequence.
ACCESSION   DAOPMW010000396 DAOPMW010000000
VERSION     DAOPMW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36977_length_3167_cov_7.195051"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLU11_14130"
     CDS             complement(<1..293)
                     /locus_tag="PLU11_14130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60320.1"
                     /translation="MILLLLTCILFIGYSLLLFRFRQSWEQLALFQPGHPASTRISVI
                     IPARNEEHNIGPLLLALQQQSYPAAFTEIIVVDDHSTDRTAEIVQSFPAVILIR"
     gene            complement(313..1134)
                     /locus_tag="PLU11_14135"
     CDS             complement(313..1134)
                     /locus_tag="PLU11_14135"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN60321.1"
                     /translation="MKQQVITYRSSSIHFTVEGQGEKTMVCLHGYGESAKNFEFLQHY
                     ADNLSFTIIAIDLPFHGQTEWREKEPLYADDLNSIIESIIQVTSGSNHIPYMLTGFSL
                     GGRLALSCYQSAPERIEKLVLIAPDGLKVNGWYWFATQTWAGNQLFALTMKKPYWFFG
                     LLKLMKKLNLVNHSIFKFVNHYISDDSVRHSLYLRWTQLRKLKPDLSLIKKQICEHHT
                     PVRLLYGKHDRIIVTSVGHKFRAGIESCCTLREINCGHQVLHEKHSKEILNAIQQ"
     gene            complement(1194..2456)
                     /locus_tag="PLU11_14140"
     CDS             complement(1194..2456)
                     /locus_tag="PLU11_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN60322.1"
                     /translation="MNKTCFCLLLVLFCTSLQSPLSAQKKVVVMGSSTAMGSAATSSA
                     LSWVGRMRTHYQNNATDGVDTSFLLVGGYGYVTYNQMPGSFTPPPGRPPHIPGYNVDQ
                     ALSLAPDIVIINLPNNDVASGFTKKEVMDNLRYLYSYILSNGTTGIQCYICTSQPRND
                     LPFAQRDSLRTLKDSILLNFGNYAINFWDDLVTNDGQYLLKDEVRHIGFPDADYHLNN
                     QGHQVIFQEARDKNIFSPNILLPVIFKNFLAQKHGTQSRLSWQVEAEEAFTIYQVERS
                     TDGQHFSTLHTRQAQAGSGQVSYTWTDEQPAIGKNLYRIKITENNRVRYSPVMAVIFE
                     EREITIDKLYLNTGFNALVMEIHCRKSQKTQISIRNTLGVITDQFTYTITAPYTTLNI
                     PLTMTSSGLYFITLTDENGKSCSKAFIR"
     gene            2655..3095
                     /locus_tag="PLU11_14145"
     CDS             2655..3095
                     /locus_tag="PLU11_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPN60323.1"
                     /translation="MPNNQFKRGELYSFITGKASTAIARRLQKKFNAAGLNITIEQWS
                     VLYHLWKQEGISQQELCNATFRDKPSITRLVDNLEKLNLVKRVASESDRRINMIYLTK
                     QAQKLQEDTMALAEETLNEALETVPADKVDICKEVLQIVYDNLK"
BASE COUNT          892 a          635 c          768 g          872 t
ORIGIN      
        1 cggatcagga ttactgccgg gaaggattga acgatttctg cggtacggtc ggtagaatga
       61 tcatccacta caataatttc tgtaaaagca gcaggataac tttgttgctg caaagcaagg
      121 agcaagggac cgatattgtg ttcttcattg cgggccggaa taattacact gatccgggta
      181 gatgcaggat gaccgggttg aaataatgcc aattgttccc aggattggcg gaagcggaac
      241 agcaggaggc tgtacccaat gaataatata caggtgagca aaagcaggat catgcggctg
      301 ggacaaaact aatcattgct gaatagcgtt caggatttct ttactatgtt tttcatgcag
      361 cacctggtga ccgcaattga tttcccgcag ggtacagcag gactcgattc cggccctgaa
      421 tttatggcct actgatgtta caatgatgcg gtcgtgtttg ccatataaga gacggacagg
      481 cgtatggtgc tcgcaaatct gttttttaat cagggataaa tcaggtttta attttcttaa
      541 ttgtgtccag cgcagataca gggaatgcct gactgagtca tcactgatat aatgatttac
      601 gaatttaaaa atactgtggt taaccaggtt gagtttcttc atgagtttta acaggccaaa
      661 gaaccagtaa ggctttttca tcgttaaagc gaaaagttgg ttcccggccc atgtttgggt
      721 ggcaaaccag taccagccat tcacttttaa gccatcgggg gcaatcagta ccagtttttc
      781 gattcgttcg ggggctgatt gataacagga aagggccagc cggccgccga ggctgaaacc
      841 tgtaagcata tacggtatat ggttgctgcc tgaggtaacc tgtataatgc tttcgataat
      901 agagttgaga tcatcggcgt acaggggctc tttttcccgc cattctgttt gtccatgaaa
      961 gggaagatca atcgcaataa tggtaaaaga gaggttgtct gcatagtgct gcaggaattc
     1021 aaaatttttc gcggattctc catacccgtg caggcaaacc attgtttttt cgccttgccc
     1081 ttctacggtg aaatgaatgg aagaagacct gtacgttatt acctgttgtt tcattgtatc
     1141 aggtattgaa gatacaggtc ttctttcagt cttacggtta tgctgtcaga aaatcaacgg
     1201 ataaaagcct ttgaacagga ttttccattt tcgtcagtca gcgtaataaa gtataaacct
     1261 gaactggtca ttgttaaagg aatgttcagg gtcgtatagg gagctgttat agtatacgtg
     1321 aattggtctg taatcacacc gagggtgtta cgtatactga tctgtgtttt ttgtgatttc
     1381 cggcaatgta tttccataac caatgcgtta aaaccggtat tcaggtaaag tttatcaatt
     1441 gtaatttccc gttcctcaaa aattacagcc ataaccgggg aataacgtac cctattgttt
     1501 tcagttattt taatccggta caggtttttt ccgatagccg gttgttcatc ggtccaggta
     1561 taacttacct ggccgctgcc tgcctgtgcc tgcctggtat ggagagtaga aaaatgttgt
     1621 ccgtccgtac ttcgttcaac ctggtaaatg gtaaatgctt cttctgcttc cacctgccag
     1681 ctgagccggc tttgtgtacc atgcttttgt gccagaaagt ttttaaaaat aacgggaagc
     1741 agtatgttgg ggctaaatat gtttttgtcc cttgcttcct ggaaaatcac ctggtgtccc
     1801 tgattgttga ggtggtagtc ggcatccgga aaaccgatat gccggacttc atctttaagt
     1861 aaatactggc cgtcattggt taccaggtca tcccagaaat taatggcata atttccaaaa
     1921 tttaaaagaa ttgaatcctt tagtgtccgc agtgaatccc gctgggcaaa ggggaggtca
     1981 ttccggggtt gtgaggtgca gatataacac tggatgccgg ttgtgccatt ggacaaaatg
     2041 tatgaataca ggtatcgaag gttgtccatg acttcttttt ttgtaaatcc ggatgccaca
     2101 tcattgtttg gtaaattaat gatgacaata tcaggggcca gacttaatgc ctggtccaca
     2161 ttgtatcccg gaatatgcgg tggccgcccg gggggagggg taaagctgcc gggcatctga
     2221 ttataggtaa cataaccata acccccaacc aggagaaagc tggtatccac cccatcggtt
     2281 gcattgttct ggtaatgcgt ccgcatccgg ccaacccagc ttaatgcaga agaagtggct
     2341 gctgaaccca tggcggtgga tgaacccatt acgaccacct ttttctgcgc tgagagaggt
     2401 gattgcaggg acgtgcagaa taataccagt aacaggcaga aacatgtttt attcatataa
     2461 caatttttgg gtagtattca gggacggaga aagccagcaa tcagagtctg cccagggcgg
     2521 gacacattaa aattaaaagg tatggattta agaaatttaa gtacatatac ttagttgttc
     2581 acaaaaaaga aacagttaag gtttttccgg aatgaggatt tttcatatat ttgaatagtt
     2641 gcacaaacaa ctatatgcca aacaaccaat ttaaacgagg cgagctgtac agctttatta
     2701 ctggtaaagc gtcgactgct atcgcccgca gactgcagaa gaaatttaat gcggccggac
     2761 tgaatatcac gatcgagcaa tggagtgtat tgtatcattt gtggaaacag gaggggatca
     2821 gtcagcagga attgtgcaat gccactttcc gggataagcc gagtatcacc cgcctggtgg
     2881 acaacctgga aaagctgaac ctcgtaaaaa gggtggcttc ggaaagtgac cgccggatca
     2941 atatgattta cctgaccaaa caggcccaga aacttcagga agataccatg gcactggctg
     3001 aagaaacact gaatgaagcc ctggagactg taccggccga taaagtggat atctgtaagg
     3061 aagttttaca gatcgtgtat gacaacctga aataagccca accattttaa atatataact
     3121 tctaaaacgc ctcattatga gtaccgcaac agaaaccagg aaagcac
//
LOCUS       DAOPMW010000397         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_36980_length_3167_cov_6.506427, whole genome shotgun sequence.
ACCESSION   DAOPMW010000397 DAOPMW010000000
VERSION     DAOPMW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36980_length_3167_cov_6.506427"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PLU11_14150"
     CDS             complement(<1..1393)
                     /locus_tag="PLU11_14150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN60324.1"
                     /translation="MHTRFLIGLLFISVFSFAQNLKSGGRLKAEQAIMDIRHITIALD
                     VDPEKKTIDGYTETDLVLAEPSGVLLFDLWHEMKVTKIWINGKAASFTHTPDDLVRIE
                     SGGILPAGNVKVKIAYGGTPGIAVRPPWTGGFQWEKDAKGNPWIAVSCQSEGAKVYFP
                     CKDHPSDEPNEGAEMIITVPKGLVVAAPGLLQKQSTKKNKTTWHYKTNYTISNYCLVF
                     NVGNYKVAKRVYTSVTGKKIPMEYYVLEENLDKAEKLLDLFEQSSRILEKYFGEYPWA
                     NERIAASETPHLGMEHQTNIAYGNKYRYEKLGSYDFDWLLHHEYGHEWWANKVTNRDW
                     GHMWIQEGICVFGDAMATRELAGEEAYQQRMQQTALNTQNKFPVVRGDEVDSDSAYHG
                     DIYGKGAFFMHMLRFVIGDDVFFPTLKRLSTEPKYVYDNTVVTADVEQLFSSASGKSL
                     QPLFKLFLYTTDKL"
     gene            1528..2037
                     /locus_tag="PLU11_14155"
     CDS             1528..2037
                     /locus_tag="PLU11_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60325.1"
                     /translation="MYFVLHKSRFRLMILISAVFLLSCNSSEKYKSPEELGKTVFEMF
                     QKKETSQLKALLISKSEIETAFGSSPQNQSLTEEERKVFINSTYERNKEIGEGFIDFY
                     TGGDPATTKMLASGKLEKTTSDIRNDNGTSVAWIVNHFTVDGKSVELNLRAAKVKEYW
                     KLLSWIEVK"
     gene            complement(2062..2961)
                     /locus_tag="PLU11_14160"
     CDS             complement(2062..2961)
                     /locus_tag="PLU11_14160"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HPN60326.1"
                     /translation="MIRIVSLTAMILLSSSLAGQRVDWKKIQAAHPDKLLLDGERRPV
                     PVLLLGAFHFGYPNLDGHKTDSAKYIDVKSPVRQKEMEELAAVIMRFKPTRIYVEAND
                     GNWIDSLYDAYRSGNYKLGRNEIFQLGFRVAGLSDHKKVYSVDTWPFSSEFESRYPWI
                     DSLWSHNTPVDSVRDQYWAAKYKRWYDAGDGQELKMTLLENFLNMAEPATLQRMHGHY
                     LASGFSTAEEVNGNGQQGPDALSIWWYNRNLRIFNNILRTKPGPEDRILVLFGNGHMP
                     ILRHCFYSSPEFRVVELKTLLKK"
BASE COUNT          849 a          776 c          672 g          870 t
ORIGIN      
        1 ccagtttatc cgtggtatat aaaaataatt taaacaatgg ctggagtgat ttgccagaag
       61 cggaactgaa taactgttca acatcagccg tcactaccgt attgtcatac acatattttg
      121 gttcggttga taatcgtttc agtgttggaa agaagacgtc atcccctatc acaaaacgaa
      181 gcatatgcat aaagaaagcc cctttgccat agatgtcccc atgataagca ctgtctgaat
      241 ccacttcatc gccccgtacc accggaaatt tattttgggt attgagtgcc gtctgctgca
      301 tacgttgctg ataagcttct tccccggcca gctcacgggt ggccatggca tccccaaata
      361 cacaaattcc ttcctggatc cacatatggc cccaatcgcg gttcgtgact ttattcgccc
      421 accattcgtg tccgtattca tgatgcagca gccagtcaaa atcgtagctg cccagttttt
      481 catatcgata cttattgcca tacgcgatgt tcgtctggtg ctccatcccc aggtgcgggg
      541 tttcagaagc ggcgatccgt tcattggccc aggggtactc tccgaagtat ttctcgagga
      601 tacggctgga ttgttcaaac agatccagca atttctccgc tttatccagg ttttcttcga
      661 gtacataata ctccatcggg atcttctttc cggttactga agtgtatacc cttttggcga
      721 ctttataatt tcctacattg aataccaggc agtaattgct gatggtataa tttgtcttat
      781 aatgccaggt ggttttattc tttttggtgc tttgtttctg caacaaaccg ggtgccgcta
      841 ctaccagtcc tttaggcacg gtgataatca tctcggcgcc ttcgttgggt tcatcgctgg
      901 gatgatcttt gcagggaaaa tagactttgg ccccttcact ctggcaactc actgcaatcc
      961 agggatttcc ttttgcatcc ttctcccact gaaagccacc ggtccagggg ggacgcaccg
     1021 caatacccgg ggtcccacca taggcaattt tcacttttac attaccagca ggtaaaatac
     1081 cacccgattc tatccgtacc aggtcatccg gggtatgagt gaagctggct gcctttccat
     1141 tgatccagat cttcgtcact ttcatttcgt gccacagatc aaataacaaa accccactag
     1201 gttccgccag caccaggtcg gtttcggtat acccgtcaat ggttttcttt tccggatcca
     1261 catccagggc gatagtaata tgacggatat ccataatggc ctgttcggct ttgagccggc
     1321 caccggattt aagattctgg gcaaaagaaa acacggaaat aaacaaaagt ccaatcagaa
     1381 aacgggtatg catgggaact gttttgggga agttacagca attccggcaa ctgggaaacg
     1441 ggtaaaataa atattgctga caagaagcag cctgccctac accgggctaa atttgtatat
     1501 acaattttta caatttaacc ttcagttatg tattttgtgc tgcataagtc ccgcttccgt
     1561 ctgatgatcc tgatatccgc tgtttttttg ctttcctgca actcttctga aaaatacaaa
     1621 agtccggaag aattgggcaa gaccgtattt gaaatgtttc agaaaaagga aaccagccaa
     1681 ctgaaagccc tgcttatcag taaatccgaa attgaaaccg catttggcag cagcccgcaa
     1741 aaccaatcac tcacagaaga ggaacggaaa gtttttatta actccactta cgaacgcaac
     1801 aaggaaatcg gcgaaggctt tattgatttt tacaccggtg gcgatccggc caccacaaaa
     1861 atgctggcca gcggcaagct ggaaaaaacc acctcggata tccggaatga caatggaact
     1921 tcagtagcct ggatcgtcaa tcactttacg gtagacggta agtccgttga actgaatctg
     1981 cgggcggcca aagtaaagga gtattggaag ctgttaagtt ggatagaggt gaaataatca
     2041 atcgccagta tgccaattgt ttcatttttt taaaagggtt tttaattcca ccaccctgaa
     2101 ttcaggagac gaataaaaac aatgcctgag aataggcata tggccattcc cgaataaaac
     2161 aaggatacgg tcttccggac cgggttttgt tcgcagaata ttattaaaga tccttaggtt
     2221 ccggttatac caccagatag acagtgcatc cggtccttgt tgtccgttcc catttacttc
     2281 ttccgctgta ctgaaaccgg aagcgagata atgcccgtgc atacgttgca aggtggcggg
     2341 ttctgccata ttgagaaaat tttccaacag ggtcattttt aattcctggc catctccggc
     2401 atcataccac cgcttgtatt ttgcagccca gtactgatcc cgcaccgaat ccaccggggt
     2461 gttatggctc cacaatgaat caatccaggg gtacctggac tcaaactcac tggaaaaggg
     2521 ccaggtatcc actgaataga ccttcttatg gtccgacagt cccgccaccc ggaatcccaa
     2581 ctgaaaaatt tcatttctcc ctaatttata gttacctgaa cggtaagcat catacaagga
     2641 atcgatccaa ttaccatcat tagcctcgac atagatacga gttggtttaa atcgcatgat
     2701 gacagcagcc agctcctcca tctccttttg ccggaccggt gatttcacat caatatattt
     2761 tgccgaatct gttttgtggc catcgaggtt aggatacccg aaatgaaaag caccaagcaa
     2821 taaaacgggt acaggccggc gctctccatc cagcaacagt ttgtccggat gcgccgcctg
     2881 aatttttttc cagtccactc tctgaccggc taatgaagat gaaagtaaaa tcatcgctgt
     2941 taaactgaca attctgatca tagcttgtgt tttaggagat aaaaatccaa tgctttcttc
     3001 gccttttaaa atccgatctg catacccgtt ttttttgtct gccaaaaaaa atcaggtccc
     3061 ggcttgcaga taaaaagttg agtttcgcag acatctcatg gatttgcgct gcggcttcag
     3121 tagatttgat ggatgagaaa cagcacgctc ctcctggtct atgccgc
//
LOCUS       DAOPMW010000398         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37105_length_3160_cov_5.932045, whole genome shotgun sequence.
ACCESSION   DAOPMW010000398 DAOPMW010000000
VERSION     DAOPMW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37105_length_3160_cov_5.932045"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..582)
                     /gene="rdgB"
                     /locus_tag="PLU11_14165"
     CDS             complement(7..582)
                     /gene="rdgB"
                     /locus_tag="PLU11_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPN60327.1"
                     /translation="MKLIFATNNQHKVDEIRAVLKGPLELITLKEAGINIDIPEPYNT
                     LEENARTKSSTIFELTGSHCFSEDTGLEVEALGGEPGVKSARYAGEEASFEANIDKLL
                     DKLNGTGNRRARFRTVISLLINGQETLFEGICEGNITIERRGNNGFGYDPVFVPDGSD
                     LSFAEMDMEGKNRFSHRKKATQKLVAFLNSL"
     gene            complement(592..1143)
                     /locus_tag="PLU11_14170"
     CDS             complement(592..1143)
                     /locus_tag="PLU11_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60328.1"
                     /translation="MDINHIQLSPVALAGLYKNHLVESGDSSPGAGRSVPVTEPPLID
                     LSASAAATAITVKALGNNEKKVLVLVRHTDVVFLPDEELKFLTGILAACKLSIADVSI
                     VNLHHAPGTGYKDLLTQFHSSRVLLFGLEPGDINLPLSFPEFQLQAFNGCTYHWCPDL
                     GRMENDRALKTRLWNNLKILFTI"
     gene            1215..2279
                     /locus_tag="PLU11_14175"
     CDS             1215..2279
                     /locus_tag="PLU11_14175"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPN60329.1"
                     /translation="MTAAMDISVTRVERSKLQDLDMENLPFGKYFTDHMLEADFENGE
                     WKNIEIKPYQPLLLSPSVAALHYGQAIFEGIKAYKDREGHPFIFRPQDNHKRFNISAE
                     RMQMPAVPEELFVEGMRRLISLDQAWIPMKNDFSLYIRPFMFSSDELIGVRPSDTYKF
                     MIILSPSGPYYAAPMRIYVEEKYVRAVPGGVGYAKAAGNYGAAMYATAEAKKKGYDQV
                     LWMDAFEHKYVQECGTMNVFFIIGNTAITPGLEQGTILGGVTRNSVITLLNEMGVTVE
                     ERDLSIDEIVAAYKAGEHMEVFGTGTAATISPIKELGYKDMSMTFDLDKWKIAPAIKK
                     TITDIREGRQADTHGWMYKI"
     gene            2493..2873
                     /gene="rpsL"
                     /locus_tag="PLU11_14180"
     CDS             2493..2873
                     /gene="rpsL"
                     /locus_tag="PLU11_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPN60330.1"
                     /translation="MPTIQQLVRKGREIIRAKSKSRALDQCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVKDRKQSRSKYGTKKEKAKK"
     gene            2964..>3160
                     /locus_tag="PLU11_14185"
     CDS             2964..>3160
                     /locus_tag="PLU11_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPN60331.1"
                     /translation="MRKAQAKKLPLAPDPKFNDKLVTRFVNNLMWQGKKSGAFTIFYD
                     ALDKVAKFTNEEGYEVWKRALA"
BASE COUNT          904 a          696 c          747 g          813 t
ORIGIN      
        1 gttttattac aggctattca aaaatgctac cagtttttgg gtggctttct tccggtgact
       61 gaaccggttc tttccttcca tgtccatttc cgcaaaacta aggtccgaac cgtcaggtac
      121 aaataccgga tcatagccaa aaccgttatt tccacgtctt tctatggtaa tattgccttc
      181 gcagatgcct tcaaacaggg tttcctgccc gtttattaac aaggatataa cggtccggaa
      241 acgggcccgc cggttaccag ttccattcag tttatccaat agtttgtcaa tatttgcttc
      301 aaaggaagcc tcttcaccgg catatcgggc acttttaacc cccggctccc cacccaacgc
      361 ttccacttcc agtcctgtgt cttcactaaa acagtgggag cctgtcagtt caaaaatagt
      421 ggatgacttg gtacgggcgt tttcctccaa tgtgttatat ggctcaggta tatcaatgtt
      481 gatcccggct tccttcaggg ttatcagctc caacggacct ttcagaacag cgcggatttc
      541 atcaacctta tgctggttat ttgtggcaaa aatcagtttc aatacagcaa tttatatggt
      601 aaagagtatt ttaagattat tccagagacg ggttttaagc gcccggtcgt tttccattct
      661 tccaaggtcc gggcaccaat gataggtaca tccgttgaaa gcctgaagct gaaattcggg
      721 aaaacttaag ggcagattga tatcacccgg ttcaagtccg aacagcaaca cccggctact
      781 gtggaattgg gtcagcaaat ctttataacc agttcctgga gcatggtgca ggtttacaat
      841 cgacacatcg gcaatgctta atttacaggc ggccagtata ccggtcagaa actttaactc
      901 ctcatccggc aaaaaaacaa catccgtgtg ccggaccaat accaacacct tcttttcatt
      961 attgcccagt gctttaaccg taatggccgt cgcagcagct gaagcggata aatcgattaa
     1021 aggcggttct gtaacaggaa cagacctgcc ggcacccggg gaactgtcac cggattctac
     1081 gaggtgattt ttgtataagc ctgccaatgc aaccgggctt aattgaatat gattaatatc
     1141 catagtaacg tgataaaagt actaatagct gttcgctttt ttttcgtttc tttgaggttc
     1201 aaaaataatc ttttatgacg gcagcaatgg atatttcagt aacccgggtg gaaaggagca
     1261 aactccagga cctggatatg gaaaatctgc cttttggaaa gtattttacc gaccatatgc
     1321 tggaggcgga tttcgaaaac ggtgagtgga aaaatattga aatcaagcct tatcaaccgc
     1381 tcttgctgag tccttcagta gcagccctgc attacgggca ggcgattttt gaaggaataa
     1441 aagcctataa agaccgtgaa ggccatccgt ttatctttcg tccgcaggat aaccacaaac
     1501 ggttcaatat ctcagcagaa cggatgcaga tgccggctgt ccctgaagaa ttatttgtgg
     1561 aaggcatgcg caggctgatc tcactcgacc aggcctggat tccgatgaag aatgattttt
     1621 cgctttatat acgtccgttt atgttctcat cggatgagtt gattggagtc cgtccatcgg
     1681 acacatataa attcatgatc attctgagtc cttccggccc ttattatgcc gcgccgatgc
     1741 ggatttatgt ggaggaaaaa tatgtccggg cagtgccggg cggggtaggg tatgccaagg
     1801 ccgccggaaa ttatggtgct gccatgtatg ctaccgcaga agcaaagaaa aagggttatg
     1861 accaggtgtt gtggatggat gcatttgaac ataaatatgt gcaggagtgc ggaacgatga
     1921 atgtcttctt tattattggt aatacagcga tcacacccgg actggaacaa gggacaattc
     1981 ttggaggggt tacccgtaac agtgtgatca ccctgttgaa tgaaatgggg gtaactgttg
     2041 aggaaaggga cctgagtatc gatgagattg tggcggccta taaagccggc gaacatatgg
     2101 aggtatttgg taccggaacc gcagccacta tttccccgat caaagagctc gggtataaag
     2161 acatgtccat gacttttgac ctggataaat ggaagattgc accggcgatc aagaaaacga
     2221 ttaccgatat ccgggaaggt cgccaggctg atacgcatgg ctggatgtat aaaatctaaa
     2281 aagaagcccg gagtaaaaaa tcggtcttat tcaggtgaat tggcagaaaa tcactgattt
     2341 agaagagctt ttccttgttt tagcggaacg aaaaaggttg ttttggccag atttatcccc
     2401 aaaaccggga agaatgtaaa aaacgcccct catttttttg gagattaaga cgtctgcctt
     2461 tacctttgcc ctcccaaaaa taagggtcaa gaatgcctac aattcaacaa ttagtacgaa
     2521 aaggaagaga aattatcagg gcgaagagta aatccagggc cctggatcag tgtcctcagc
     2581 gccgtggtgt ttgtaccagg gtgtatacca ctactcctaa gaagcctaac tctgcactgc
     2641 gtaaagtggc taaagtccgt ctgactaata aaatcgaggt gattgcctac attccgggag
     2701 aaggccacaa cctgcaggaa cactccatcg tactgatcag gggtggtcgt gtgaaagact
     2761 taccaggtgt acgttaccac atcgttcgtg gatcactgga tacagccggt gttaaagacc
     2821 gtaagcaaag ccgttccaaa tacggaacca agaaggagaa agcgaagaag taatccttcg
     2881 gctacgactt ccaccttcgc taatgctacg gtggacaagc aggatggcaa ataatataaa
     2941 aagagactta taataattca gatatgcgga aagcacaagc aaagaaactc cccttagcac
     3001 ccgaccccaa gtttaatgat aaactggtaa cccggtttgt aaacaacctg atgtggcagg
     3061 gtaagaaaag cggagccttt accatttttt acgacgccct tgacaaagta gccaaattca
     3121 cgaatgaaga aggttatgaa gtttggaaaa gagcgctggc
//
LOCUS       DAOPMW010000399         3159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37119_length_3159_cov_8.750000, whole genome shotgun sequence.
ACCESSION   DAOPMW010000399 DAOPMW010000000
VERSION     DAOPMW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37119_length_3159_cov_8.750000"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PLU11_14190"
     CDS             <1..1254
                     /locus_tag="PLU11_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPN60332.1"
                     /translation="TDGTVKTQVTAVGNDTVLSGILNMVKQAQGEKPPVQQLADKISA
                     IFIPVVLAIAVLTLVISWIVLKEFTPALMRSIAVLVIACPCAMGLATPAAIAVGLGRA
                     AKTGILFRNATSLETFKDIKQVVFDKTGTLTTGQFMIGDWKILQAGLTEEGFKQIVYS
                     LEKYSNHPIAKCITEQWKIKDPLQWAKVEEVKGLGMQATDKEGNLYTAGSYKSAEGLT
                     REDQHNVYVLRNGVLQGWIDVKDELRPEAKAVVDYLHRKNIKTILLSGDRQSKCNALA
                     VQLGIDTVIAEQSPEQKLHKIAALSEQVPTAMVGDGINDAPALAKATIGISMNDASQI
                     AVQSAQVILMNHGLKNLPTALGLGKHTYLTIKQNLFWAFAYNIVAIPVAALGFLTPTF
                     GALVMGLSDVVLAINSVRLFVKKVV"
     gene            1335..>3159
                     /locus_tag="PLU11_14195"
     CDS             1335..>3159
                     /locus_tag="PLU11_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPN60333.1"
                     /translation="MTDPKQILKEYWGYDAFRPMQEDIIKSVLNGKDVLALMPTGGGK
                     SLCYQVPAMAMDGLCLVISPLIALMKDQVENLRKRGITAFAIFSGMSRKEVIRTFHTA
                     MESNCKFLYVSPERLETSLFKEYLPGLGVHLIAVDEAHCISQWGYDFRPPYLRIAALR
                     TELPNVPILALTASATPEVQDDIAQKLELQQPQIYRQSFERANLSYSCFRMDSKINKI
                     IDILKKVPGTGIVYCKSRKRTKEIAELLQLQGIPADFYHAGLNQEERSAKQEAWIKNT
                     IRVIVCTNAFGMGIDKPDVRTVVHADVPDCLENYYQEAGRAGRDGKTSYAVLLYDERD
                     LKELDEMASLRFPSLEEIREVYQALANYLQLPTGSGSGQYYDFDIADFLKKFPFAPHT
                     LMNAMRSLEQEDWLSFNEQVFLPSTVIFTINKEGLYAFEGNYPDLEPCIKSLLRAYEG
                     IYDMPVAVSEIVLAGLLRKESAAVKNDLVMLDKAGVIHYQPRKDNPQVYLMRNRVKAG
                     DISIDMVAYNKRKEQFRRRMRQMIQFVQEDTQCRSRIIGHYFGDANMKDCGICDNCLK
                     QKATVLSKEEFVAIEQRLLQLLGTAPVDNKILLQQLSGIKKE"
BASE COUNT          897 a          701 c          803 g          758 t
ORIGIN      
        1 acagatggca ccgttaaaac acaggttacc gcggttggga atgacacggt actctccggg
       61 attctcaata tggtcaaaca ggcccaggga gaaaaaccac ccgtacagca actggccgat
      121 aaaatcagcg ccatctttat tccggttgta ctggctattg cagtacttac cctggtgatc
      181 agctggattg ttttaaaaga attcactccg gccctgatgc ggagtatagc tgtattggtg
      241 atcgcctgtc cctgtgctat gggactggcc acaccggccg ccattgctgt aggattggga
      301 cgtgcggcaa aaaccggtat cctgttccgc aatgccacca gcctggaaac ttttaaggat
      361 attaaacaag tggtctttga taaaaccggt acactcacta caggtcagtt catgatcggc
      421 gattggaaaa tcctgcaagc cgggcttacg gaagaaggat ttaaacaaat cgtctattcg
      481 cttgaaaaat attccaacca cccgattgca aaatgcataa ctgaacaatg gaagataaaa
      541 gacccgttac agtgggcaaa ggtggaagaa gtaaaaggat tgggtatgca ggccactgat
      601 aaagaaggga atttatatac ggccggttcc tataaatccg ccgaaggact gaccagagaa
      661 gaccaacata atgtttatgt acttcgtaat ggggtattac aaggctggat cgacgtgaaa
      721 gatgaactgc gtccggaagc gaaagcagtg gtagattatc tgcaccgcaa aaacataaaa
      781 accattctgc tgagtggcga ccggcagtca aaatgcaatg cactggccgt acaactcggt
      841 attgataccg tgattgccga acagtctccg gagcagaagc tgcataagat tgcagcactc
      901 agtgaacagg tgcctacggc tatggtgggg gacggaatta atgatgcccc ggcactggcc
      961 aaagcaacaa tcggcatctc catgaatgat gcgtctcaga tcgccgtaca aagtgcccag
     1021 gtgatcctga tgaatcacgg attgaagaat ctgcccactg cgctgggatt aggcaaacat
     1081 acctacctca caattaaaca aaaccttttc tgggccttcg cgtataacat tgtggccata
     1141 ccggtggctg cgctcggttt tcttacacct acatttggtg ccctggttat gggactgagt
     1201 gatgtggtac tggcaatcaa ctcggtgaga ttatttgtca agaaagtggt ataaaacaaa
     1261 atccgggatc tgatcccgtt ccggaaaagg aactcccgcc acaataatgt aatttgtcgc
     1321 ctgcaataaa tcacttgaca gacccgaagc aaatactgaa agaatactgg ggctatgatg
     1381 cttttcgccc gatgcaggaa gatattataa agtctgtact gaacgggaaa gatgtgctgg
     1441 ccctgatgcc gaccggcggt ggtaaatcac tttgctacca ggtccctgcc atggcgatgg
     1501 acggactttg cctggtgatc tctccattga tcgccctgat gaaggatcag gtggagaacc
     1561 ttcgcaaaag agggatcact gcttttgcta ttttctccgg tatgagccgc aaggaagtga
     1621 tccgcacgtt tcataccgcc atggagagta attgtaaatt cctgtatgta tcgccggaac
     1681 gattagagac cagcctgttt aaagaatacc tgcccggatt aggcgtacac ctgatagcag
     1741 tggatgaagc gcattgtatt tcacaatggg gctatgattt tcgtccaccg tatttgcgta
     1801 ttgccgctct gcggaccgaa ttacctaatg tgcccatcct ggcacttacc gcttcggcca
     1861 cacctgaagt tcaggacgat attgcacaga aactggaatt acaacagccg cagatttacc
     1921 gtcagtcatt tgaaagggcc aacctttcct atagttgctt ccggatggat tccaagatca
     1981 ataagatcat tgatattctg aaaaaagtac cgggtaccgg tatcgtgtat tgcaaaagcc
     2041 ggaaacgcac caaagaaatc gctgagctgt tacaactgca aggcataccc gcagattttt
     2101 accatgccgg actcaaccag gaagaacggt ccgccaaaca ggaagcctgg atcaaaaata
     2161 cgatccgggt gattgtctgt accaatgctt ttggcatggg gatcgataaa ccggatgtaa
     2221 gaactgtagt acatgccgat gtgccggatt gtctggagaa ttattatcag gaggccggaa
     2281 gggccggccg ggatgggaag acctcgtatg cggtattgtt gtatgatgag cgggatttga
     2341 aagaactgga cgaaatggcc tcccttcgtt ttccctcgct tgaagaaatc agggaagtat
     2401 accaggcttt ggccaattac ctgcagttac caacgggaag tggcagcggg caatattatg
     2461 attttgatat tgctgatttc ctgaaaaaat tcccatttgc tccacacaca ttgatgaatg
     2521 cgatgcggtc gctggaacag gaagactggt tgtcatttaa cgaacaggta ttcctgccct
     2581 ccacggtgat ctttacgatc aataaagaag ggctgtatgc atttgaagga aattacccgg
     2641 atctggaacc ctgtatcaaa tccctgttaa gggcttatga gggaatctat gatatgccgg
     2701 tggccgtttc agaaattgta ttggcgggtt tgctccgcaa ggaatcggcg gcggttaaaa
     2761 atgatttagt aatgctggat aaggccggcg tgattcacta ccaacccaga aaagataatc
     2821 cccaggtgta cctgatgcgt aaccgcgtga aagccggtga tatatctatt gatatggtgg
     2881 cgtataataa acggaaagag cagtttcgcc gccggatgag acagatgatt cagtttgtgc
     2941 aggaagacac ccaatgccgg agccggatca ttggccatta ttttggcgac gccaacatga
     3001 aggactgcgg catctgtgat aattgcctga aacaaaaagc aacggtactg agtaaagagg
     3061 agtttgtcgc cattgagcaa cggttactac agttactggg aacagctccg gttgataata
     3121 aaatcctgct gcaacaactg agtggcatta aaaaagaaa
//
LOCUS       DAOPMW010000400         3156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37182_length_3156_cov_7.239923, whole genome shotgun sequence.
ACCESSION   DAOPMW010000400 DAOPMW010000000
VERSION     DAOPMW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37182_length_3156_cov_7.239923"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLU11_14200"
     CDS             <1..268
                     /locus_tag="PLU11_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPN60334.1"
                     /translation="FLRIYAWHSFDPSLYGIYWWQIGVTVSVTLLGVVLWGTLSGSML
                     PLLLKRLGADPAVSSAPFVATLVDVTGLILYFTVAYAMLSGSLL"
     gene            383..2218
                     /gene="glmS"
                     /locus_tag="PLU11_14205"
     CDS             383..2218
                     /gene="glmS"
                     /locus_tag="PLU11_14205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPN60335.1"
                     /translation="MCGIVGYTGPREAYPIIIKGLKRLEYRGYDSTGVALQNGGLHVY
                     KKKGKVAELEESIVGKNLHSHIGIGHTRWATHGEPSDRNAHPHQSSSGQLAMIHNGII
                     ENYNQLKKELINKGYTFSSDTDTEVLLNFIEEIKKNNECSLEEAVRVALKRVTGAYVI
                     LLMSADDPETIIAARKGSPLVIGVGKGEHFLGSDASPMLEYTKEVVYVNDYELAIVKP
                     DELILKNLGNEKLTPYVQKLDIELAAIEKGGYEHFMLKEIFEQPQTVYDCLRGRLDAT
                     EGTITMSGIQQYAEQIMNANRIVMVACGTSWHAGLVAEYIFEELCRINVEVEYASEFR
                     YRNPVINKGDVIIAISQSGETADTLVAIENAKNNGAIILGVVNVVGSSIARMSHAGAY
                     THAGPEIGVASTKAFTAQLAVLTMIALKIGYIKGSITQKRYMNLLKELQEIPEKVAWT
                     LKHSDNIKALAEKYKDAHDFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALVDETLPVVFVATKDSFHEKVVSNMQEIKARKGRVISVITEGDEMSASLSEDVMV
                     VPEADEILAPMLSVIPLQLLAYYIGLARGCDVDKPRNLAKSVTVE"
     gene            2234..>3156
                     /locus_tag="PLU11_14210"
     CDS             2234..>3156
                     /locus_tag="PLU11_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPN60336.1"
                     /translation="MNIITKKAISSLGYGFIPKEFIPKGKNEYYLRNIQHRSGIRYRK
                     LTPVEIQLLEKNDNSSDDWNKVLVSESFDPLLVKRCSFFGLVRIGKLEPYFHEFNNLH
                     MPVGLYNSTIISCDIGDNVVIDNVSFMSHYIIGNDVMLVNINELATTNHSKFGNGVLK
                     EGEKEAVRVWMEICNENGGRSILPFNEMQAGDAWLWSKYRDDEKLLQKFMEFTDKKFD
                     TRRGYYGKIGDRTVIKNCKIVKDTWIGSDAYLKGANKIKNLTINSSPEAKSQIGEGCE
                     LVNGIIGYGCRIFYGVKAVRFMMGSNSQLKYG"
BASE COUNT          884 a          722 c          786 g          764 t
ORIGIN      
        1 cttcctgcgg atctatgcct ggcattcttt tgacccttca ctctatggaa tctactggtg
       61 gcagatcggg gtcactgttt ctgtaactct cctgggggta gtcctctggg gtactctttc
      121 cggcagtatg ctccccctcc tgctaaaaag actgggtgcc gaccctgcgg tcagttccgc
      181 cccctttgta gccaccctgg ttgatgtaac cgggctgatt ctttacttca cagtagctta
      241 cgccatgttg agcggcagcc ttctttaata cgttaaggct ttgacttcta caattaaatt
      301 cgcaccttta cacttatgca ggcagtcaaa cgtttgcatg tttagaaatt gatacttttg
      361 acgcaaaaaa ccatcaaacc ttatgtgtgg aatcgttggc tataccggac ccagggaggc
      421 ttatcctatt attatcaaag gcctgaaaag gctggaatac cgcggttatg acagtaccgg
      481 ggtggcgctg caaaacggcg gcctgcatgt ttataagaaa aaaggaaaag tcgcggagct
      541 ggaagaaagc attgtcggca aaaacctgca ctcccatatc ggcatcggcc atacccgctg
      601 ggccacccac ggtgaaccca gcgaccggaa tgcccaccca catcagtcct cttccggcca
      661 gctggccatg attcataacg gcatcattga gaattacaac cagctcaaaa aagaactgat
      721 taataaaggg tatacttttt ccagtgatac cgacaccgaa gtactcctca attttattga
      781 ggaaatcaaa aagaataatg aatgttccct ggaagaagca gtccgcgtgg cactgaaaag
      841 ggtaaccggt gcttatgtga tcctgctgat gtctgccgat gatccggaaa ccatcattgc
      901 cgcccgaaaa gggagtcccc tggtgatcgg tgtgggcaaa ggggaacatt ttctgggttc
      961 ggatgccagc ccgatgctgg agtataccaa ggaagtggtg tacgtgaatg actatgaact
     1021 ggcgatagta aagcccgatg aactgattct gaaaaacctc ggaaatgaaa aactgacgcc
     1081 ctatgttcag aagctggaca tcgaactggc cgccatagaa aaagggggtt atgaacattt
     1141 catgctgaaa gaaattttcg agcaacccca aaccgtttat gattgtttac ggggacggct
     1201 ggatgcaacc gaaggtacca tcaccatgag cgggatccag cagtacgccg aacagatcat
     1261 gaatgccaac cggattgtga tggtggcctg tgggaccagc tggcatgcgg gcctggtggc
     1321 agaatatatt tttgaagagc tctgccggat taatgtggag gtggaatatg cttctgaatt
     1381 caggtaccgc aatccggtga tcaataaagg cgatgtcatc attgccattt ctcagagtgg
     1441 cgaaaccgcg gataccctgg tggccattga aaatgccaaa aacaatggtg ctatcatcct
     1501 gggtgtggtc aatgtagtgg gctcttccat tgcccgtatg agtcatgccg gtgcttatac
     1561 ccatgccggt ccggaaatcg gtgttgcgtc taccaaggcc tttaccgcgc agctggcggt
     1621 actcaccatg atcgccctga agatcggata tatcaaaggc agcattacgc aaaaacgcta
     1681 catgaacctg ctgaaagaat tgcaggagat acccgagaaa gtagcctgga cactcaaaca
     1741 cagtgataat ataaaagcac tggctgaaaa atacaaagac gcgcatgatt tcctttatct
     1801 cggccggggg tataattttc cggtagccct tgaaggggcc ctgaaactga aagaaatctc
     1861 ttacatccat gcagagggtt atccggctgc agaaatgaaa catggtccta ttgcactggt
     1921 ggatgaaacc ctgcccgtgg tatttgtagc caccaaagat agtttccatg aaaaagtggt
     1981 aagcaatatg caggagatca aggcccgtaa aggacgggtg atctccgtga tcaccgaagg
     2041 agatgaaatg tctgcttcgc tttctgaaga cgtgatggtg gtaccggaag ccgatgaaat
     2101 cctggctccg atgctgagtg taattccgct tcagttactg gcctactata tcggactggc
     2161 ccgtggctgc gatgtggaca aaccccgtaa cctcgccaaa tcagtaacgg tcgagtaaat
     2221 tttattcccg cacatgaaca tcattactaa aaaagccatt tcctcccttg gatacggatt
     2281 tattcctaaa gaatttattc ccaaaggaaa gaatgaatat tacctgcgca atatccagca
     2341 ccgcagcggt atccgctacc gtaaactaac cccggttgaa attcagttgc tggaaaaaaa
     2401 tgataacagt tcggatgact ggaacaaagt gctggtgtcg gaatcatttg atcccctgct
     2461 ggtcaaaaga tgttccttct ttggcctggt ccgtatcggc aaactggaac cctatttcca
     2521 tgaattcaac aacctgcata tgccggtggg actttacaac agcaccatca tcagttgtga
     2581 tatcggcgat aatgtagtaa tcgataacgt gagttttatg agccattata ttatcggcaa
     2641 cgatgtgatg ctggtgaaca ttaatgaact cgccaccacc aaccactcca aattcggtaa
     2701 cggggtattg aaagaagggg aaaaagaagc agtacgtgta tggatggaaa tctgcaatga
     2761 aaacgggggg cggagtatac ttccgtttaa tgaaatgcag gccggtgatg cctggctctg
     2821 gtcaaaatac cgggacgatg aaaaactcct gcagaaattc atggagttta cggataaaaa
     2881 atttgatacc cgcagaggat actatggtaa aatcggggac cgcacggtga tcaagaattg
     2941 taagattgtc aaagacacct ggatcggttc agatgcttac ctcaaagggg ctaataaaat
     3001 caaaaacctg acgatcaatt cttcgcccga agccaaatct cagatcgggg aaggttgtga
     3061 actggtcaat ggcattattg gatacggctg ccgtatcttc tacggggtga aagccgttcg
     3121 ttttatgatg ggcagcaatt cgcagctgaa atacgg
//
LOCUS       DAOPMW010000401         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37191_length_3155_cov_6.014839, whole genome shotgun sequence.
ACCESSION   DAOPMW010000401 DAOPMW010000000
VERSION     DAOPMW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37191_length_3155_cov_6.014839"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="PLU11_14215"
     CDS             complement(<1..2278)
                     /locus_tag="PLU11_14215"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPN60337.1"
                     /translation="MKRPKLLVLYFTGLLLALATVQATGQTKIIHGLVRDAHSEEPVP
                     FASVLFKNSTTGKLTDSSGQFSFYLAKLPSDTLLITCVGYQPFSLILNNLRDSSSFSI
                     LLERGTFNEGAQLKVKVNKGLLVWRKIVQNKPFNDRYRFDNFSYELYNKLELDLKNIN
                     FEKFGRFKPLKPISELINQNIDTSEGIKYLPTYLTETLSDYYYQKSPKKRREIIRAYN
                     TNGVKNESFSKMLGGMDQVVNVYNNFIPVFDKQFVSPISDNGDYYYNYRVVDTQVIAN
                     KRYFHLVFTPRRKGGDTFEGDCWVHVGSFAIQRMNLRLGKEANVNFIENLSLIQEYKQ
                     INDSTWFLSKDKFVADVSPAGKDRPGFIGRKTTTYDHIVVNDSSVINELSKNKILEEI
                     ITSPEAGKKTKEFWAMERHEPLSKTESSIIKMIDTLTNAPVFQRFTKTMAFIGTGYKD
                     IGNYKIGPWFNWITSNGWEGFRMRFDLGTNKHFDKKIKLHGYLAYGFGDKHLKGMGEI
                     FYLPKKDPRMYLYGSFTNDLDWGQNYYGEVSQDNIFALAIRKKGIPVKNIKVNEKKIE
                     FFKETFPWMSNRITVSHKSTLPLRNLVPIDSFRATASGKPLTSFEVSVRLRFAYLERF
                     LESHFYRSSLGSIYPIGEIFITQGFPGVLKSSYKYTKISASISDYFKVPPFGNISWYV
                     YGGKTFGTIPYTLLDIAPGNELYYYNKYAFNMMNRWEFIHDEFAGINIEHNIGNGIFR
                     LLPKLRWRQFWTAKTLWGS"
     gene            2406..3050
                     /locus_tag="PLU11_14220"
     CDS             2406..3050
                     /locus_tag="PLU11_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60338.1"
                     /translation="MEEKLPGTSSLNDLSDIRRIMERSSRFVSLSGLSGVAAGICAIG
                     GAWVANRYFSNYYGAYNSRGFTTGDDFSQLKLKLMLLGAVVFLLAFASSYYLTWRKAR
                     KAGLPVWDHTSKRLAWNMLVPLMAGGAFIIGMLRYDVWIFVAPACLMFYGLALVNASK
                     YTLSDIRYLGYCEIVLGTVNMLYPGYGLWFWAAGFGVLHIIYGVIMWNKYERNN"
BASE COUNT          800 a          657 c          773 g          925 t
ORIGIN      
        1 ggcttcccca gagtgttttg gccgtccaga actggcgcca gcggagttta ggcagaaggc
       61 ggaagatgcc gttaccaata ttatgctcga tattaatccc ggcaaattca tcatgtatga
      121 attcccagcg gttcatcata ttgaaagcat atttattata atagtacaat tcattacccg
      181 gtgcaatatc caataaggtg taggggatgg tgccaaatgt tttcccgcca tatacatacc
      241 aggatatatt tccgaagggt ggaactttaa agtaatctga aatactggcc gatatcttgg
      301 tgtatttata actgcttttc aacacacccg gaaagccctg ggtgatgaag atttcaccga
      361 tcgggtagat actcccaaga ctgctccggt aaaaatgact ttcaagaaaa cgttccaggt
      421 aggcaaagcg gaggcggaca gatacttcaa aactggtcag cggctttccg ctggcagttg
      481 cccggaatga atcaatgggc accaggttgc gtaagggcag tgtggattta tgtgaaacgg
      541 tgatccggtt cgacatccag ggaaaagtct ccttgaagaa ttcgatcttc ttctcattca
      601 ctttgatgtt ttttaccggg atcccttttt tacggatggc caaagcaaat atattatcct
      661 gcgatacttc gccatagtag ttctgtcccc agtccaggtc attggtaaaa gaaccataga
      721 ggtacatgcg gggatctttc tttggcaggt aaaagatctc gcccatgcct ttcaggtgtt
      781 tatctccaaa cccataagcg agatagccat gtagtttaat ttttttatca aaatgcttat
      841 tagtgcccag gtcaaaacgc atccggaatc cttcccagcc gttggaagtg atccagttga
      901 accaggggcc gatcttgtaa ttgccgatat ctttataacc ggtgccgata aaggccatgg
      961 ttttagtgaa tcgctggaat acaggggcat tcgtcagggt atcgatcatt ttaatgatac
     1021 tgctttccgt tttgctcagc ggttcatgtc gttccatggc ccagaattcc tttgtttttt
     1081 ttccggcttc aggcgaggtg atgatttctt cgagtatttt attcttgctg agttcattga
     1141 ttaccgaact gtcattaacc actatatgat catatgtggt cgtttttcgg ccaataaatc
     1201 caggacgatc cttacctgca ggggatacat cagccacaaa cttatcctta gagaggaacc
     1261 aggtgctgtc attgatctgt ttgtattcct ggatcaggct caggttttca ataaaattga
     1321 cattagcttc cttacccagg cggagattca tccgttgaat ggcgaaactc cctacatgta
     1381 cccaacaatc gccttcaaaa gtgtctcccc cttttcgacg gggcgtgaat accaggtgga
     1441 agtagcgctt attggcaatg acctgggtgt ccaccaccct gtaattataa tagtagtcgc
     1501 cgttgtcgct gatcggactc acaaactgtt tgtcaaaaac cgggatgaag ttattgtaga
     1561 cattcactac ctggtccatg ccccccagca ttttcgaaaa actttcattt tttactccat
     1621 tggtattgta ggcccgaatg atttcccttc tttttttcgg tgatttctgg tagtaataat
     1681 cggagagtgt ttcggtgagg taagtgggca ggtatttgat accttccgaa gtgtcaatat
     1741 tctgattgat cagttcgctg atcggtttga ggggtttaaa ccggccaaat ttttcaaaat
     1801 tgatattctt caggtccagt tcaagtttgt tataaagttc gtaagagaaa ttatcaaagc
     1861 ggtagcggtc attaaagggt ttgttttgca cgattttacg ccagaccaat aatcccttgt
     1921 tcacttttac tttcaattgg gcaccttcgt taaacgttcc tctttccagc aggatggaaa
     1981 aagaggagga atcccggaga ttattcagga tcaggctgaa aggttgatag cccacgcagg
     2041 taatcagcag agtatcggag ggcagtttag caagatagaa gctgaattga ccagaagagt
     2101 ccgtgagttt accggtagtg ctgtttttaa aaagtacgga ggcaaagggg actggttctt
     2161 cgctgtgagc atcccggacc aggccatgga tgatttttgt ttgtccggtg gcctgaactg
     2221 ttgccagtgc cagcagaagt cccgtaaaat agagtaccaa cagtttcggt cgcttcataa
     2281 agtggtaaaa ttacagcgcc ttcgggtata gtggaatgtt aaattcggtg aaaggccgga
     2341 attgaccgga aaaaatttgg agagatgcgg aaatttcaca tactttgtat tacaaagtgc
     2401 tttttatgga agaaaaatta cccggaacca gctccttaaa tgacctcagt gatatccgcc
     2461 ggattatgga acggtccagt cgttttgttt cgctcagcgg actgagtggg gtcgcggctg
     2521 gtatttgtgc cattggcggg gcatgggtgg ccaatcgtta tttcagcaat tattacgggg
     2581 cctataatag tcgcggattt accaccggag atgatttcag ccaactgaaa ctgaaactaa
     2641 tgctgctggg agcggttgtt tttttactag ctttcgcttc ttcttattat ttaacctggc
     2701 gcaaggcccg taaggcaggt ttgccggtat gggaccatac ttcgaagcgg ctggcctgga
     2761 atatgctggt gccgctgatg gcgggtggcg cttttataat cgggatgctg cgctatgatg
     2821 tctggatatt tgtagcaccg gcctgcctga tgttttacgg cctcgccctg gtcaatgcga
     2881 gcaaatacac gctttccgat atccgttacc tgggctattg cgaaattgta ctaggtacgg
     2941 taaatatgct ttaccccggt tatggactct ggttctgggc ggctggtttt ggggtgctgc
     3001 acattatata tggcgtgatc atgtggaata aatacgaacg aaataactga gcatagaacg
     3061 ttacgacatg aagaatccaa tcaccggttt aaataaaata tttgacaacc gcatccggct
     3121 gggggtgatg agtgtgctca tggtcaatga ggaga
//
LOCUS       DAOPMW010000402         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37510_length_3135_cov_6.793182, whole genome shotgun sequence.
ACCESSION   DAOPMW010000402 DAOPMW010000000
VERSION     DAOPMW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37510_length_3135_cov_6.793182"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLU11_14225"
     CDS             <1..749
                     /locus_tag="PLU11_14225"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPN60339.1"
                     /translation="VVTARKPEQVADIVAAYPDTALSLTLDVTVPQQINDAVKTAIDR
                     FGRIDVLVNNAGIGYFGAIEESEEEEVRRMFEINFFGLAHMTNAVLPHMRKQRSGHIL
                     NVASIGGLRSFPAVGFYNATKYAVDGLSEALMKEVAPLGIKVTVICPSGFRTDWAGRS
                     ANNTKIKIEDYATTAGKNMSDLRGYSGNQPGDPVKAARAMIQVTETENPPLRLLLGAA
                     ALKGARLKLEELRKDFDDWAEVSEGADFKS"
     gene            804..3119
                     /locus_tag="PLU11_14230"
     CDS             804..3119
                     /locus_tag="PLU11_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HPN60340.1"
                     /translation="MKSFLTGITVFGLIAVMGALSCLHQQEGTDNMPVTVSYNFHIRP
                     ILSDKCFKCHGPDASKRKASLRLDIADSAYAPLKETKGAYAIIPGKPEASELYLRVIS
                     TDTAYMMPSPEEHLGALTPYETALIKKWIQQGAKYETHWAFIPPVKAPLPAIKDKDKA
                     SNEIDYFILSKLQEKGLDPSEEADKERLLKRVSMDITGLPPSTEMMDRILKEPSSATY
                     EKLVDELLNTPQYGEKMAIHWLDVARYADSYGYQDDNIRTQWPWRDWVIHAFNKNLPY
                     DQFLTWQIAGDMLPDAGKEQILATGFFRNHKYTEEGGVIPEEYRIEYLIDKTKTFGKG
                     IQGITIECAQCHDHKYDPFKQTDYFSLMAFFNNTKEKGFEGDVSVSTPAKFPVLPIND
                     SDVQKVLSFINKKDTGIVTVSVMAEDTLRKTYRLNRGVYDQPAEEVTASAIPAVMKFD
                     STKYPRNRLGLAKWTTDKQNPLTARVFVNQVWQEIFGRGLVKTTGDFGMQGELPTHPE
                     LLDWLSVDFMEHGWNIKRLIKQLVMSATYRQSARTTTEKQKKDPENIYLSRGPRNRLP
                     AELVRDLVLSSSGLLVKTIGGPSVKPYQPKGLWEGATSGRGVLATYKQDKGEDLYRRG
                     MYTFIKLTVPPPGMILFDASNRDQCEVKRSKTNTPLQALIMMNDPTILEASRVLAQRL
                     LAEPGAAEDKIQQAFRLIICRKAVKEETKILTAYFNEQLALFRQNKLDAAATLKAGEY
                     PPANFKDKPVLAALMKTINTIYNLEEAITKT"
BASE COUNT          928 a          725 c          745 g          737 t
ORIGIN      
        1 tagttgtgac tgcccgaaaa cctgaacagg tagcagatat tgtagccgct tatcccgata
       61 ctgctttatc gctgacactg gatgtaaccg ttccccaaca gataaatgat gccgtaaaga
      121 ccgctattga ccgctttggc cggatcgatg tactggtgaa caatgcgggg atcggttatt
      181 tcggtgcgat cgaagaaagt gaggaagaag aagtacgccg catgtttgaa attaatttct
      241 tcggtctggc acacatgacc aatgcggtgt tgccccatat gcgtaaacaa cgcagcggtc
      301 atatattaaa tgtagcgtct attggcggac tgcgttcctt cccggctgtt ggcttctaca
      361 atgccaccaa gtatgccgtt gacggtttaa gcgaagcact catgaaagaa gtagccccat
      421 taggtattaa agtaacggtg atttgcccga gtggtttccg taccgactgg gcaggacgtt
      481 ccgccaataa tactaaaatt aagattgagg attacgcaac gacagcgggt aaaaacatga
      541 gtgatctccg tggatatagt ggcaaccaac ccggcgatcc ggtgaaagca gccagggcca
      601 tgatccaggt aaccgaaacc gaaaaccctc ccctgcgttt actgttagga gccgctgccc
      661 tgaaaggtgc aaggctcaaa ctggaagagt tgagaaagga ttttgatgac tgggcggagg
      721 tgagtgaggg tgctgatttt aaaagttaac gtgaaaaccg gtttttagga aagattctta
      781 acttcaacag atatagttgc cacatgaagt ctttcctgac cggtattact gtttttggtt
      841 taattgctgt tatgggagcc ctctcctgcc ttcaccagca ggagggtacg gataacatgc
      901 ctgtcaccgt cagctataat tttcatatac gccccatact ttccgacaaa tgctttaaat
      961 gccatggtcc ggatgcttct aaacgaaaag cctctctccg gttggatatt gccgatagcg
     1021 cctatgcccc gctcaaagaa acaaaaggag cctatgccat aatacctggc aaacccgaag
     1081 catcagaact ttatttacgg gtcatttcca cagataccgc ctatatgatg ccttcccccg
     1141 aagagcacct gggggcactg actccctatg aaacagccct gataaaaaaa tggattcagc
     1201 aaggcgcgaa atatgaaacc cattgggcct ttattccccc tgtaaaagca ccccttcctg
     1261 caattaagga taaggacaaa gccagcaacg aaatagatta ttttatcctg agtaaactgc
     1321 aggaaaaagg cctggatcct tcggaagaag ccgataagga acgcttattg aagcgggtct
     1381 caatggatat taccggatta ccgccctcaa cagagatgat ggaccgtata ttgaaagaac
     1441 cttcatccgc tacttacgaa aaactggtgg atgaattact caataccccg cagtatggag
     1501 aaaaaatggc catccactgg ctggatgtag cgaggtatgc agacagttat ggttaccagg
     1561 atgataatat aagaacccaa tggccctggc gtgattgggt catccacgcc ttcaataaaa
     1621 atttacccta tgaccagttt ctgacctggc agatcgccgg ggatatgctg cccgatgccg
     1681 gaaaagaaca gatactggct accggttttt tccgaaatca taaatacacg gaagaaggtg
     1741 gcgtgatccc ggaagaatac cggatagaat acctgataga caaaaccaaa acctttggca
     1801 aaggaataca gggaattacc attgaatgtg cccaatgcca cgatcataaa tacgacccgt
     1861 ttaaacaaac cgattatttt tccctgatgg ctttctttaa taatacaaaa gagaaagggt
     1921 ttgagggaga tgtctctgtt tctacaccgg caaaatttcc tgtgctgccg atcaacgata
     1981 gtgatgtaca gaaagtgttg tcttttatca ataaaaaaga taccggtatc gtaacggttt
     2041 cggtaatggc tgaagacacg ttgcggaaaa cgtatcgcct gaaccgtggt gtatatgatc
     2101 agcccgccga ggaagtcacc gcttccgcca tccctgccgt tatgaaattt gattccacca
     2161 aatatccgcg taaccggctt ggattggcta aatggacaac ggataaacaa aatccgttga
     2221 ctgcaagagt atttgtcaac caggtatggc aggagatatt cggaagagga ttggtgaaaa
     2281 ccacgggtga ttttggtatg cagggtgaac tgcccaccca ccccgaattg ctcgactggc
     2341 tgtccgtgga ttttatggag catgggtgga atatcaaacg actgattaaa caactggtga
     2401 tgtcggctac ctatcgccag tccgccagga ccacgactga aaaacaaaaa aaggacccgg
     2461 agaatatcta tttatccaga ggaccgagaa accgtttgcc cgctgaactc gtacgcgacc
     2521 tggtgttatc cagcagtggt ctgctggtaa aaacaatcgg cggtcccagt gtaaaaccct
     2581 atcaacccaa agggctgtgg gaaggagcta cttccggaag aggagtgctg gccacttaca
     2641 aacaggataa aggagaggat ctttaccgaa ggggtatgta cacttttatc aaattgaccg
     2701 tacccccgcc gggtatgatt ttatttgatg cgagtaaccg tgatcagtgt gaggtaaaac
     2761 gttccaaaac caatactccc ttacaggccc tgatcatgat gaatgatccg accatcctgg
     2821 aagcttcgcg ggtactggcc cagcgtttat tagcagaacc gggcgctgcc gaagacaaaa
     2881 ttcaacaggc ctttcgctta atcatttgcc gtaaagcagt caaagaagaa acaaaaattc
     2941 tgactgccta tttcaatgaa caactggccc tgttccggca aaataaactg gatgccgcgg
     3001 ccaccctgaa agcaggtgaa tatccgcctg cgaattttaa agacaaaccg gtgctggccg
     3061 ccctgatgaa aaccatcaac accatttata acctggaaga agccattaca aaaacctgaa
     3121 catgcagaaa gagta
//
LOCUS       DAOPMW010000403         3134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37529_length_3134_cov_6.599545, whole genome shotgun sequence.
ACCESSION   DAOPMW010000403 DAOPMW010000000
VERSION     DAOPMW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37529_length_3134_cov_6.599545"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLU11_14235"
     CDS             complement(<1..507)
                     /locus_tag="PLU11_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HPN60341.1"
                     /translation="MKKFITLTLIAFFATSLFAGRIIHSYNSISPTAKDVGNLNMIMF
                     EGMYLTGKTGEPVLPYQAVKLLLPPGEEAVSVIWKFENELIIAGNFTLYPQQPVQPYS
                     VGAQGIFQQNMDVYSSGNVYPAEAMGRYSTHFLNGHGFLLATFTPVKYIPATGQVSFF
                     QKVTIQVET"
     gene            786..1106
                     /locus_tag="PLU11_14240"
     CDS             786..1106
                     /locus_tag="PLU11_14240"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPN60342.1"
                     /translation="MADEKVINIIRRYVYLLDNEGLGIDKAFLYGSYASGSNSENSDI
                     DLMLVSGLISDNDIQKKSRAWILTKDVDTRIEPYLISTTRFTKDDISPLIEVVKREGI
                     EILL"
     gene            1468..2715
                     /locus_tag="PLU11_14245"
     CDS             1468..2715
                     /locus_tag="PLU11_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60343.1"
                     /translation="MKNLIEMMLVAVFMSGCAEIMTEEEMPDKWNGYFQYLKTGEMVH
                     TLWAGKNINVGTVTYGIDENANFYATYDCSAPGWKMSETHMYAGTKAGMPLNKPGRPK
                     IGQFPNSGCHSPRVSTFTYRVPLSSLPPCASPGFVVAAHCVVHGPGGKTESAWAEGSY
                     TFSDKGWGWYDDYYYDPPQEPGPVLYGTSCANDSLRIYHLDISTGCVEMILEEYVGGS
                     PGFYDGADYDPVTGLLFFIKYTTSELWINHLQGEEPSFCAGILSGPSTCGTFQGDHYY
                     YINNSTGSIQSVSFTSTWLIAEEMPLDTLPAGYAVNDIAMDSSGESLYILAQNQSGSM
                     QLLRWDAGEPAFFSMPVAVSEDAQIAFGSDGLLYAVSSTGECDTCFYIYTIDLENGML
                     EFVEDDVIMFDDPFSDLSRGPSL"
     gene            2790..>3134
                     /locus_tag="PLU11_14250"
     CDS             2790..>3134
                     /locus_tag="PLU11_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60344.1"
                     /translation="MKKLITICMILSGILFAFPVLAQVNKNKPVNKESTSDKKPGTIT
                     ANTKGATATKMFDAAGTLIFTHDQMGYVRNARNRVFFQYTAAGDIIKKRAVIGSVKNG
                     IFRDRLGKEYARI"
BASE COUNT          891 a          704 c          722 g          817 t
ORIGIN      
        1 ggtttcaacc tgtatggtaa ctttttgaaa gaaagaaacc tgacccgtgg cagggatata
       61 tttcaccggt gtaaaagtgg ccaggagaaa cccgtgacca ttgagaaagt gggtggagta
      121 ccgtcccata gcctctgcgg ggtaaacatt acccgaagaa taaacatcca tattctgttg
      181 aaatattcct tgagcgccga cagaatacgg ttgaaccggt tgttgcgggt acagggtaaa
      241 attaccagca ataattagct cattttcaaa tttccaaata accgagaccg cctcttcgcc
      301 cggagggagc agcaatttca ctgcctggta gggcaaaacc ggctctccgg tcttccccgt
      361 caggtacatc ccctcgaaca taatcatatt aagatttccc acatctttcg ccgtaggcga
      421 tatactattg tagctgtgaa tgatgcggcc ggcgaagagc gatgtggcaa agaaagcaat
      481 cagggtaagg gtaatgaatt ttttcatcgg ggttggagat ttgaaatgag cgtctaaata
      541 tttttaaaat tccattcaaa ataccgttac aatattaaat atttctatga tcagtaatac
      601 attcaaaggt taaatggata ataaatgatc aaaaaaaact aaaagcctta tttttgtctc
      661 cctcatttga tgctgaatat agaaaagcag atacaatact ggattaacgg ttcaaacgaa
      721 gatttgatca ccagtgaaat atttctggtt cttgaaattt tcactcagac aaaagaattt
      781 caccaatggc tgatgaaaaa gttataaata ttataaggag atacgtttat cttcttgata
      841 atgaaggtct tgggattgac aaagcattcc tttatggcag ctatgcttcc ggctctaatt
      901 ccgaaaacag cgatattgac cttatgctgg tatccggact gatttccgat aatgatattc
      961 agaaaaaatc ccgggcatgg atattgacaa aggatgtcga cacacggatt gaaccctatc
     1021 tgatcagcac aacccgtttt actaaggacg acatttcacc tttaattgag gtcgtgaaaa
     1081 gggaaggtat tgagattctg ttgtagaatc actatttctt catgcttact aattatactc
     1141 attctttgca ggtttgcagc agcaaagctg cttagaatga cttataccgg ggagaacaac
     1201 tttgttaatt cgcaaaccgc ttcagaatcg gtataacaat cgccaatcaa ttcgcagaac
     1261 tacccactgc cgactatcga ctgccgactg cctactctac tctccctcaa taagttaaaa
     1321 aaatatttcc tctccaccgg gtattttttg gtcaaaaacc ggattaatag ataagaaagc
     1381 gatcatccct ctgcccgacc gatctgccgg attaaatttt ccatcccacc ccagttcact
     1441 gaattttaat ccttaaaaca ctttcttatg aagaatttaa ttgaaatgat gctggtggca
     1501 gttttcatgt cgggttgtgc tgaaatcatg acggaagaag aaatgccgga caaatggaac
     1561 gggtatttcc aatatctcaa aaccggggag atggtccata cattgtgggc cggtaaaaac
     1621 atcaatgtcg gcacggtcac ttacgggatt gatgaaaatg ccaacttcta tgccacttat
     1681 gattgcagcg ccccgggatg gaagatgtcg gaaacacata tgtatgccgg aaccaaagca
     1741 ggaatgccgc tgaataagcc gggcaggccc aagatcggtc agttccccaa ctcgggatgc
     1801 catagtccgc gggtaagcac atttacttat cgcgtgccac tgtcgtcgct tcccccgtgc
     1861 gcctcgccgg ggtttgtcgt ggccgctcac tgtgtagttc atggccccgg aggaaaaacc
     1921 gagagtgcct gggccgaggg cagttatact ttcagcgaca aaggctgggg ttggtacgac
     1981 gactattact acgatccgcc gcaggagccc ggaccggttc tgtatggcac ctcctgcgcc
     2041 aacgactcct tacggatcta tcacctggat atctcaacag gttgtgttga aatgatcctg
     2101 gaagaatacg tcggcggcag ccccggattt tatgacgggg ccgattacga tccggtgacc
     2161 ggtttgctgt tttttatcaa atacaccact tccgaattat ggatcaacca cctgcagggt
     2221 gaagagcctt cattctgtgc cggcatcctg tcagggccat ccacctgcgg gacgtttcaa
     2281 ggggatcact attactacat caacaattcc accggatcga tccaaagcgt gagtttcacc
     2341 agtacctggc tgattgcgga ggaaatgccc ctggatacac tgccggccgg ctacgcagtc
     2401 aatgatatag ccatggattc ctccggcgaa agcctgtata tcctggcgca aaaccaatcg
     2461 ggcagcatgc agttgcttcg ctgggatgcc ggggagccgg cctttttcag catgccggta
     2521 gcggtcagtg aggatgcaca gatagctttt ggcagcgatg gattgcttta tgccgtgagt
     2581 tcaacgggtg aatgcgatac ctgcttctat atttatacca tcgacctgga gaacggaatg
     2641 ctggagtttg ttgaagacga tgtgattatg ttcgatgatc ctttcagtga tctctcgcgc
     2701 gggccatcgc tctgatttgg gaaaacaagc ttattttata ctaattttgg atttttaatg
     2761 ttaatgttaa aaatatattc caaattaata tgaaaaagct gattacaata tgtatgatcc
     2821 tgtcgggaat cctttttgca tttccagtat tggcacaggt taataaaaat aaaccggtca
     2881 acaaggaaag tacgtccgac aaaaaaccgg gaacaattac ggcaaacacc aaaggggcaa
     2941 cggccaccaa aatgttcgat gctgccggaa ccctgatctt cacgcacgac cagatgggat
     3001 acgtaagaaa tgcccgcaac cgggtatttt tccagtacac ggcagccggc gacatcatta
     3061 agaaaagagc ggtcatcggt tctgtgaaga acggcatttt ccgcgacagg ctgggaaaag
     3121 agtacgcccg tata
//
LOCUS       DAOPMW010000404         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_37745_length_3123_cov_7.429270, whole genome shotgun sequence.
ACCESSION   DAOPMW010000404 DAOPMW010000000
VERSION     DAOPMW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37745_length_3123_cov_7.429270"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLU11_14255"
     CDS             <1..957
                     /locus_tag="PLU11_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN60345.1"
                     /translation="IYKVNDASVPSTYTWTVNGITQPGNRHELPITWTTAGVYQLAVQ
                     EHANNGCDGDIRTGTVYVMPTPVANAGPDAIVCYGKTLRLNGSGGLQYQWSPATYLSS
                     TAIAGPLVTLPAAGVYTYVLNVSANGCPALKPDTVKVTMLPPVRVFAGNDTLIALSQP
                     LQLNAQDMNNSGFTSYHWSPSFGLNNALVKTPVATLNSLGSTTYLVTARTADGCEASD
                     DIKITAFVKADLYVPTAFTPNGDGKNDQAVVIPVGIRELQFFRIYNRWGELVFMTQDA
                     ARGWNGVFKGQPQDSNVFVWEAKGVDYNGNVIFKKGTVTLIR"
     gene            complement(1062..2201)
                     /locus_tag="PLU11_14260"
     CDS             complement(1062..2201)
                     /locus_tag="PLU11_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60346.1"
                     /translation="MPVCAQKNPGRTSFEGPPPPPANWKSPKGIANTTSPTDLSYLVP
                     GIKLVPEKKPVYKPKSYYIEQTIDSSFSDSAGFAILPRDYRIQPIRFEGKKNLLFQKY
                     FESVVVKNKSLLPLTLVLKRYQIEEKQVNKADLDISLALCFDVYCRVDSQLCLIQHIG
                     DTLQAEGYVGSKRKYDSLLKVHIRQFLEITDEFTGPFKEFVEHAENTQALVQLAAPKD
                     ESEDADTLYIDDKRYLQWSDFRGTPKQKGIAFGNYGLFLTANILPQDNKVVAAITIYP
                     VFIRNSSWADEDSRSGRLLNHINYELLLTYSHALQLKKKLHNQTFPANKMNEQLNRLL
                     DQEIELLKIERELYFKETQSGQNVKAQAEWEEKIGNDLKQYSKDQ"
     gene            complement(2310..2594)
                     /locus_tag="PLU11_14265"
     CDS             complement(2310..2594)
                     /locus_tag="PLU11_14265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60347.1"
                     /translation="MTIHHKQAGGKGLFFVGEEGAYLAEMVYTLPSTDKMIIEHTEVS
                     EELRGQNVGYQLVQAAVEHARTYSMKIIPLCPFANAVFKKKPEYADVLFS"
     gene            2820..>3123
                     /locus_tag="PLU11_14270"
     CDS             2820..>3123
                     /locus_tag="PLU11_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPN60348.1"
                     /translation="MKKITINTRSKRMLADVYTPVGIYLRLRDKFRDTILLESTDHHS
                     SENSYSFICINAIGGMEVSSKESIEFKYPGRNPEKASLKNTAEVPGLLWNYMQQFDV"
BASE COUNT          780 a          675 c          774 g          894 t
ORIGIN      
        1 atctacaaag tgaatgatgc gtcggtacct tctacctata cctggacagt aaatggaatt
       61 acccagcccg gcaaccgcca tgagttgccc attacctgga ctactgccgg ggtataccag
      121 ctggccgtcc aggagcatgc gaataatgga tgcgatgggg atatccgtac cggtacggtc
      181 tatgttatgc caacaccagt agccaatgca gggccggatg ccatcgtatg ttatggtaag
      241 accttgcggc tgaacggtag cggcggattg cagtatcagt ggagtccggc tacttattta
      301 tccagcacgg cgattgccgg tccgttggtt actttacccg cagccggggt ctatacgtat
      361 gtgttgaatg taagtgccaa tggatgcccg gcattaaaac ccgatacggt aaaagtgacg
      421 atgttgccgc cggttcgcgt gtttgccggt aatgatacac tgatcgcatt gagccagcct
      481 ttacaactca atgcacagga tatgaacaac agtggattca ccagttacca ttggtcacct
      541 tccttcggtc tgaataatgc cctggtcaaa acacctgtgg ctaccctcaa cagcctgggc
      601 agtacaacct atctggttac ggcccgtacg gccgatggtt gtgaagccag cgatgatatt
      661 aagatcaccg cttttgtgaa agccgatctt tatgttccga ccgccttcac acctaatggt
      721 gatggaaaga atgaccaggc cgtggtgata cccgtaggta ttcgtgaatt acaattcttc
      781 cggatctaca accgctgggg cgaactggtc tttatgaccc aggatgcggc ccgcggatgg
      841 aacggcgttt tcaaaggaca accccaggac agcaatgtgt ttgtatggga agccaagggg
      901 gtggattata acgggaatgt gatttttaag aaggggacgg tgacgttgat aaggtaggga
      961 gagtggggaa tggggaatgg ggaatagtga ataaaaatag gaaggaggtt actttgaaag
     1021 gtgacctcct tttttttatt gaaaataaat ggttgaacga gttattgatc tttggaatat
     1081 tgcttaaggt cgttgcctat cttttcttcc cattcagcct gagctttaac attctggccg
     1141 gactgggttt ctttaaaata taattcccgt tcaattttaa gtaattcaat ttcctgatcc
     1201 agcagtctgt tgagctgttc attcattttg tttgcgggaa atgtctggtt gtgtagtttt
     1261 tttttcagct gcagggcatg actatatgtg agtagtagtt cataattgat atggttgagc
     1321 agcctgccgg accgtgagtc ttcatctgcc caggagctat tacggatgaa aaccgggtag
     1381 atagttattg cggcaactac tttgttgtcc tgcgggagaa tgtttgcggt gaggaataat
     1441 ccgtaattac caaaagcgat gcctttctgt tttggtgttc ccctgaagtc cgaccattga
     1501 aggtatcgtt tatcatcgat gtataatgtg tctgcatcct cgctttcgtc cttaggtgct
     1561 gccagttgta caagagcctg ggtgttttcc gcatgctcaa caaattcttt aaacggtcct
     1621 gtaaattcgt ctgtgatttc aaggaattgc cggatatgca ctttcagcag gctgtcgtat
     1681 ttgcgtttac ttccaacata gccttccgcc tgtaacgtgt cccctatatg ctgtatcagg
     1741 cacaattggc tgtcgacccg gcaatagaca tcaaagcaaa gtgccaggct gatatccaga
     1801 tcggctttat tcacttgttt ttcctcgatc tggtatcttt ttaagacgag ggtaagagga
     1861 agcagcgatt tgtttttaac cactacggat tcaaaatatt tttggaataa taagtttttt
     1921 tttccttcaa aacggatggg ttgaatgcgg taatcacgtg gaaggattgc aaagccggca
     1981 ctgtccgaga aactggagtc aatggtttgt tcaatgtaat agcttttcgg tttatacacc
     2041 ggtttttttt ctggcaccag tttgatcccg ggtaccaggt aactcaggtc agtcggggag
     2101 gtggtattgg ctatcccctt tggagatttc cagttagcgg gcggcggggg tggtccttca
     2161 aaactggtcc ggcccggatt tttttgcgca caaacaggca ttgtcaaaaa actggggagg
     2221 accagcaaaa gtatagcgga tctacagaat acccgctgat gcgaaatttt cataaagaat
     2281 acaactttac aaatcagtat tatgctctct tagctaaaca acacatccgc atattccggt
     2341 ttcttcttaa acaccgcatt ggcaaaggga caaagcggga tgatcttcat gctataggtc
     2401 cgggcatgtt ctacggcggc ctgcaccagc tggtagccta cgttctgacc tctgagttcc
     2461 tcactaactt ccgtatgttc gatgatcatt ttatcggtag aaggcagcgt ataaaccatt
     2521 tctgcgaggt aagcgccttc ttcccccaca aagaatagcc ccttgcctcc tgcttgtttg
     2581 tgatggatag tcatagacgt tgttttctta aagctatcaa gatttttttg ctttttcgga
     2641 tttgtcgcca catatttgca gtagcaatga acgttacaac aacaaataat cagtggtggt
     2701 ggcatacaac tcttaagggg ctgtagtgtc atgactgcat ttgtatatca ttcaggagcc
     2761 ccggtattcg gggctcctgt tttttttacc tgccacaggc agcaagcgtg agaaccttta
     2821 tgaagaaaat aacgatcaat acccggagca aaaggatgct ggcggatgtg tataccccgg
     2881 tggggatcta ccttcgtctt cgggataaat tccgggatac gattctgctg gaaagcacgg
     2941 atcatcattc ttcggagaac agctattcct ttatctgtat caatgccatt ggcggaatgg
     3001 aagtgtcttc caaagagtca attgagttca aatatccagg ccgtaacccg gaaaaagcat
     3061 ccctgaaaaa caccgcagaa gtgccaggac tactctggaa ctatatgcag cagtttgatg
     3121 tca
//
LOCUS       DAOPMW010000405         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_38408_length_3086_cov_7.446057, whole genome shotgun sequence.
ACCESSION   DAOPMW010000405 DAOPMW010000000
VERSION     DAOPMW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38408_length_3086_cov_7.446057"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLU11_14275"
     CDS             complement(<1..421)
                     /locus_tag="PLU11_14275"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPN60349.1"
                     /translation="MKKGFPFLLFTLLFLQATAQQNNEITKNESNGLTYMREEEKLAR
                     DVYDSMYAKWDVNPFGNIRQSERVHMDRMKTLMTRYEVKDPVEATGDKPGVFKTAVLQ
                     KVYNELVKSGTTSLTDALKAGAKIEELDIADLDERIAQ"
     gene            complement(453..986)
                     /locus_tag="PLU11_14280"
     CDS             complement(453..986)
                     /locus_tag="PLU11_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60350.1"
                     /translation="MLRKTQILTWLVVLLLVLNAVTIGTIIYHQRQEKKAISDISIGG
                     YAGTNPLNGRFFRQEMGFNTQQMDVFRELNQAFRPASMEITFLIDSLKEVMYNQLTQA
                     KSDSLQLQQLSEEIGALHSQLKKETVLFYIRLKEICTPDQQKQLAEVFKPLFITEHLT
                     DHGNYRNGPGWKRKQTD"
     gene            complement(988..1299)
                     /locus_tag="PLU11_14285"
     CDS             complement(988..1299)
                     /locus_tag="PLU11_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60351.1"
                     /translation="MKKETDIENRIEQLIREERNTEPNPYLSTRVMAAIAKQQDKKAG
                     LLPMFLKPILVMASLTIVVWLGIIAGSAYTSGSTEQATVLTNDATSENFAFYLQTEKE
                     E"
     gene            complement(1341..1877)
                     /locus_tag="PLU11_14290"
     CDS             complement(1341..1877)
                     /locus_tag="PLU11_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN60352.1"
                     /translation="MQETELIQSILNGQTADFSQLVKTYETAVFRIVMGFVHQKEDAE
                     DITQEVFVKAFQSLDRFAGRSSFSTWLFRIAIHTSLNELKRRKRRTLWSMAADLLQLP
                     SSNKDPEQAFAQKTEQELVRKAIDTLPKKQRMAFILSRYEELPQRDVAQIMEISEGAV
                     EQLLLRARDNLKNFLQRP"
     gene            2157..>3086
                     /locus_tag="PLU11_14295"
     CDS             2157..>3086
                     /locus_tag="PLU11_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPN60353.1"
                     /translation="MKRTIRFYRLMWTRHDLPAGLTVFLVALPLCLGIANASGAPVYA
                     GLLSGIIGGLIVSFLSGSHLSVSGPAAGLTTIVAGSIISLGGYSVFLLAVVVAGVFQL
                     LLSVLKAGKISNYFPSSVIKGMLAAIGIMLISKQIPMALGLDKPDFWNSGFSQLFTST
                     EFSSNFKNLNQHITRGALIITVVSLAILIIWQQPFAKKLRVIPAPLAVVITGILLHLL
                     FTHGMPGFFLRPTQSVLVPSNVFAEIRFPDFSQLFSNVEIWKAGLLIGGLASLETLLC
                     VEAVDKLDRHNRITPVNRELMAQGVGNIACGLLG"
BASE COUNT          709 a          720 c          692 g          965 t
ORIGIN      
        1 tttgtgcaat ccgctcatcc aggtcggcaa tatccagttc ttcaattttt gctcctgctt
       61 tcagtgcatc ggttagcgat gtcgttcctg attttaccag ttcattatac actttttgta
      121 aaacggctgt tttgaataca ccgggtttat ctcctgtggc ttccaccgga tctttcactt
      181 cgtaccgggt catcagtgtt ttcatgcgat ccatgtgcac tcgttcactt tgacggatat
      241 ttccaaaggg gttcacgtcc cattttgcat acatggaatc atatacatcc cgggccagct
      301 tttcttcctc acgcatatac gtgagcccgt tactttcatt ctttgtgatt tcattgtttt
      361 gctgagcggt tgcctgcaaa aaaagcaggg tgaataacag gaaggggaat ccttttttca
      421 tgatgaactg ttttatgatt ataaattcag ggttagtctg tttgttttct tttccacccg
      481 ggaccgttcc tgtaatttcc atgatccgtc aggtgttctg ttataaaaag cggcttaaaa
      541 acttccgcca gttgtttttg ctgatcagga gtgcatattt ctttgaggcg tatataaaaa
      601 agtacggttt cttttttgag ctgactgtgc aaagccccga tctcttcgga taattgttgc
      661 aactgcaggc tatcggattt tgcctgggtc agctggttat acatcacttc ttttaatgag
      721 tcaatcagga aggtgatttc catggaggcc ggtctgaatg cctggttcag ttcacggaaa
      781 acatccatct gctgtgtatt gaatcccatt tcctgccgga aaaaacgtcc gttgagcgga
      841 ttagtgccgg catacccgcc tatgctgata tccgatatcg cttttttctc ctggcgctga
      901 tgatagatga tggttccgat ggtaacggca ttcagcacga gtaataaaac caccagccag
      961 gttaatattt gtgttttgcg cagcatccta ttcctccttt tctgtttgca ggtaaaaagc
     1021 aaaattttct gaggtggcgt cattggtaag aactgttgcc tgttctgtgc ttccggatgt
     1081 ataagcgctg cctgcgataa tacccagcca tactactatt gtaaggctcg ccatcaccag
     1141 tattggtttc agaaacattg gcaacaaccc ggccttttta tcctgctgtt tcgctattgc
     1201 tgccattacc cgggtagaca aatagggatt cggctccgta ttccgttctt cgcggatcag
     1261 ctgctctatc ctgttttcta tatccgtttc ttttttcata atcgctcctg gttaattaga
     1321 caatggttga aatgggtttc ctatgggcgc tgtaaaaaat ttttgagatt atcccgcgcc
     1381 cggagcaata actgctcgac cgcaccttcc gaaatctcca ttatttgtgc cacatccctt
     1441 tgtggaagtt cttcataacg gctgagaatg aaggccatac gttgtttttt gggcagcgtg
     1501 tcgatggctt tacggaccag ctcctgctcc gtcttttggg caaaagcctg ttcgggatcc
     1561 ttattggagg aaggcagttg cagcaaatcg gccgccatgg accataaggt ccggcgcttt
     1621 cttcttttca gttcatttaa actggtatgt atagcaatac gaaaaagcca ggtggaaaaa
     1681 gaggagcggc cggcaaagcg gtcaagcgac tgaaaggctt ttacaaaaac ttcctgggtg
     1741 atatcttctg catcttcttt ctgatgcaca aaacccataa caatgcgaaa cacagcggtt
     1801 tcatacgttt ttaccagttg ggaaaagtca gcggtttggc cgtttagaat ggattgtatc
     1861 agttccgttt cctgcatcag gcagatattt tgccgaaacc cggttaaagg tatttcatcc
     1921 catacatacg agaagacaga cccgggaaat gtaaagaaga tgaatttatc cctttacaac
     1981 agtaactaag gtcacgggta accgggtttt aaatcagctg ctgtactgat gtttttgctt
     2041 aagaaaacag gagtgaggat gaataaataa caatcaccca tgccattacc gggcccctaa
     2101 tggatatccg gtgtttattt tttatctttg tccctacact caactcttta tttactgtga
     2161 agcgcacaat caggttctac cggctgatgt ggacaagaca tgacctgccc gccggtctta
     2221 ctgtattttt agtagccctc ccgctttgtc ttggtatcgc gaatgcctcc ggcgctccgg
     2281 tatatgcggg cttactctct ggaataatcg gtggacttat tgtttccttt ctcagtggct
     2341 cccatctcag tgtttcgggt ccggcagcgg gtctgaccac tattgttgct ggctccatca
     2401 tctcgctcgg tggttactcc gttttcctgc tggctgtggt agttgcgggt gtattccagt
     2461 tattattaag tgtactgaaa gcaggtaaaa tttccaatta cttcccctct tctgtaatca
     2521 aaggcatgct ggctgccata ggtattatgc tgatctcaaa gcagattcca atggcgttgg
     2581 gacttgataa acctgatttc tggaacagcg ggttctccca acttttcact tccactgaat
     2641 tcagcagtaa ttttaaaaat ctgaatcagc atatcacccg cggagccctt atcattacgg
     2701 ttgtgtcatt ggccatcctt attatctggc aacaaccttt tgccaaaaaa ttacgggtaa
     2761 ttcctgcacc actggccgtg gtcataaccg gtattttgct gcatttattg tttacacatg
     2821 ggatgccggg ctttttcctg cgtcccaccc aatcagtgct tgtcccctcc aacgtatttg
     2881 ccgaaatacg atttccggac ttcagccagt tattctccaa tgtggagata tggaaagcgg
     2941 gcttactgat cggtggactc gccagcctcg aaactctttt atgcgtggag gcggtagata
     3001 aactggaccg gcacaaccgg atcaccccgg taaaccgcga actgatggct cagggggtgg
     3061 gtaatattgc ctgcggatta cttgga
//
LOCUS       DAOPMW010000406         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_38617_length_3076_cov_3.617345, whole genome shotgun sequence.
ACCESSION   DAOPMW010000406 DAOPMW010000000
VERSION     DAOPMW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38617_length_3076_cov_3.617345"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1950
                     /locus_tag="PLU11_14300"
     CDS             58..1950
                     /locus_tag="PLU11_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPN60354.1"
                     /translation="MHKLITFLLFSALPGLLIAQTADPETQYMKDNYEKFEYRIPMRD
                     GAKLFTLVYMPKDKSKTYPILMNRTCYNASNYGGYNTHGHPSNYLVHDGYILVFQDVR
                     GRYMSNGLFNNMTPNIPGNDPMNKKDVDESSDTWDSIDWMVNNLAGNNGRVGIYGISY
                     PGFYSAASLPDAHPALKAVSPQAPISDFFFDDFHHMGAYLQSYTAAFAVFGYQKKDTT
                     RNDWFNSEIMRMYSKPVKDGYDFHLKLGPLKNVTDKYHHDNFFWNQIVDHPNYDTFWQ
                     KRSILPHLKNTKPAVLTVGGWFDAEDLYGPLNIYKTIEKNTPGAKNSIVMGPWGHGDW
                     ARENGKSTHNHIYFGDSISTFFQKEIERNFFNFHLKAEGEMSLPEAYMFNTGKKEWQK
                     FDVWPPADIPPVKLSFGENGKLLINQPGNAKLSFEYTSDPAKPVPYTSQTEGLTFTPR
                     NYMSDDQREASRRTDVLTFETDVLTDEVTLAGEILAKLKVALSTTDADFVVKLIDVYP
                     DNHPNYPHNPKNVVMGGYQQLVRSEVFRGRFRNSFEKPEPFVPGKVTDVNVPLQDVLH
                     TFKKGHRIMIQIHSTWFPFIDRNPQQYVENIYKADEKDFIKSTIKVYGSSVIGAGGEQ
                     APKKAF"
     gene            1950..2453
                     /locus_tag="PLU11_14305"
     CDS             1950..2453
                     /locus_tag="PLU11_14305"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN60355.1"
                     /translation="MQDRVKQLISHYRMQPHPEGGYFLQTYKSSGSIPLSVLPEGFSG
                     SRPFSTAIFFLLEQGNFSAFHRIRSDECWHFYEGQTLLVHIITPEAEIKTVRLGPDIA
                     NGELYQFVVPANCWFASEPAPGTAYSFVGCTVSPGFDFADFEMADSAGLAAQHPRYGA
                     MIHRLCR"
     gene            complement(2456..>3076)
                     /locus_tag="PLU11_14310"
     CDS             complement(2456..>3076)
                     /locus_tag="PLU11_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN60356.1"
                     /translation="ALSLRFPAGQVMVKAVYHPLIVEKIIELAHNEDAGLIVMGTLGA
                     SGLRKVLFGSNAAHMISRSPVPVLAVPLDYVFKPVQKIVYASDLENLEAELKQIIPFA
                     IALNAVIEVVHLDYGINDKKISQKEAESIIHKSPYQFIHLLFRDADLNKPLLRQLKTH
                     LELIDPDWLIMFTKEKSMWDKLFLGSHTEDMSQALTLPLLSIKKPV"
BASE COUNT          828 a          692 c          717 g          839 t
ORIGIN      
        1 taccagtatc tactctttat taaaaggact tgttttatct tcccggaaaa aatagccatg
       61 cataagctca tcactttttt actgttttca gcccttccgg gtctcctgat tgcacagaca
      121 gctgaccccg aaacacagta tatgaaggat aactatgaga agtttgaata ccggatcccg
      181 atgcgggatg gcgcgaagtt gtttaccctg gtgtacatgc ccaaggataa atcaaagacc
      241 tatcccatcc ttatgaaccg gacctgctac aacgcttcta actatggtgg ttacaatacc
      301 catggtcatc cctccaatta ccttgtgcat gacgggtata tcctggtctt ccaggatgta
      361 cgtggtcgct acatgagtaa tggccttttt aataatatga ccccgaatat ccctggtaat
      421 gacccgatga ataagaagga tgtggatgag agttcggata cctgggacag catcgactgg
      481 atggtgaata acctggccgg caataacggc cgggtgggca tctatggcat ttcctatccc
      541 ggattttatt cagcagcctc tttacccgat gctcacccgg cactgaaagc cgtttctccg
      601 caggcaccta tttctgattt cttctttgat gatttccatc atatgggcgc ctatcttcag
      661 agttatacag ccgcttttgc cgtatttggc tatcagaaaa aagataccac ccgaaatgat
      721 tggttcaata gtgaaatcat gcgtatgtat agtaagccgg taaaagacgg ttatgatttt
      781 cacctgaaac tgggcccact taaaaatgtt accgataagt atcaccatga taattttttc
      841 tggaaccaga tcgtggatca tccgaactat gatacattct ggcaaaaacg aagtatcctg
      901 cctcacctga aaaatacaaa gccggctgta cttacagtgg gtggctggtt tgatgcagag
      961 gatctctatg gcccgctgaa tatttacaag accatcgaaa agaatacgcc cggtgcaaag
     1021 aacagtattg tgatggggcc atggggacat ggggactggg cgcgtgaaaa cggaaagagt
     1081 acccataacc atatttattt tggcgacagt atctctactt ttttccagaa agagatcgag
     1141 cgtaattttt ttaattttca cctgaaagct gagggcgaga tgtcgcttcc cgaagcctat
     1201 atgttcaata ccggtaaaaa ggaatggcag aagtttgatg tatggccgcc ggctgatatt
     1261 cccccggtaa aactcagttt tggggaaaat ggaaaattgc tcattaacca gcccggaaat
     1321 gcgaaactca gttttgaata taccagtgac ccggctaaac cggtacccta tacttcacaa
     1381 acggagggac ttactttcac gccacgcaat tatatgagtg atgaccaacg ggaagcctcc
     1441 cgcagaacgg atgtattgac tttcgaaacg gatgttttaa ccgatgaagt aaccctggca
     1501 ggggagatac tggcaaagct caaagtggcc ttatccacga ccgatgccga ttttgtcgtg
     1561 aagctgatcg atgtgtatcc cgacaatcat cccaattacc cgcataatcc aaagaatgtg
     1621 gtgatgggtg gttatcagca gttggtacgg agtgaagtat tccgtggccg gttccgtaac
     1681 agttttgaaa aaccggaacc gtttgtcccg ggaaaagtga ccgatgtaaa tgtgccgttg
     1741 caggatgtac tgcatacatt taaaaaagga caccggatca tgatccagat acacagcacc
     1801 tggtttcctt ttattgaccg caatccgcag caatatgtag agaatattta taaagcggat
     1861 gaaaaggatt ttattaaatc caccattaaa gtctatggca gttccgttat tggagcggga
     1921 ggcgagcagg cacctaaaaa agcattctga tgcaggatag ggtgaagcaa ctgatcagcc
     1981 attaccggat gcagccccat ccggaaggag gctattttct acagacatat aaatcttccg
     2041 ggagtattcc gttatcggtg cttccggaag gcttttccgg atcccggcca ttctcaacgg
     2101 ctatcttttt tttattggag cagggcaatt tctcggcctt ccaccggata agaagtgatg
     2161 agtgctggca tttttatgaa ggacagacac tgctggtcca tataataaca cctgaagcgg
     2221 agataaaaac agtccggctt ggcccggata tagccaatgg ggaattgtat cagttcgtgg
     2281 tgcctgccaa ttgctggttt gccagtgaac ctgctcccgg aacagcctat tcctttgtag
     2341 gttgcactgt ttctcccggc tttgattttg ccgattttga aatggctgat tcagccggac
     2401 ttgctgcaca gcatcccaga tacggagcaa tgattcatcg gctttgccgc tgatcttaca
     2461 ccggtttttt aatactgagc aggggcaggg tgagtgcctg tgacatgtct tccgtatggc
     2521 ttcccaggaa tagtttatcc cacatgcttt tttctttagt gaacatgatc agccagtcgg
     2581 gatcaatcag ttccaggtgc gttttcagtt gccggagtaa aggtttattc aggtctgcat
     2641 ccctgaaaag caggtgtata aactgataag gggatttgtg gataatactt tccgcctcct
     2701 tctggctgat ttttttatca ttgattccat aatccaggtg taccacttca atcaccgcat
     2761 ttaatgcaat agcaaaggga ataatctgtt taagttcggc ttcgaggttt tcgaggtctg
     2821 acgcataaac tattttctga accggtttga atacataatc cagtggaact gccagtacag
     2881 ggaccggtga acgggaaatc atatgagcgg cattcgaacc aaacagtact ttccttaatc
     2941 cgctggcacc cagggttccc atgacaatca gacccgcatc ttcattatgg gccagttcaa
     3001 ttattttttc cacgatcagc gggtgatata ccgctttaac catgacctga ccagccggga
     3061 atcttaagga aagggc
//
LOCUS       DAOPMW010000407         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_38840_length_3065_cov_7.907973, whole genome shotgun sequence.
ACCESSION   DAOPMW010000407 DAOPMW010000000
VERSION     DAOPMW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38840_length_3065_cov_7.907973"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLU11_14315"
     CDS             complement(<1..896)
                     /locus_tag="PLU11_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60357.1"
                     /translation="MKKIIIRPLLSFLLLSISLVGHSQMITGVWKGRINRQKVEVKII
                     QKGDSLTGTSYYYESASHYRRYSIKGYFDPGTNEAIWWDDQLLEEKAGRFSVSSPGKM
                     PLLARADFNCPGGSKMMLDGKATRRDDENDPKGQVDLDKAGNTDFADEWDYIIDNYMV
                     GGNDPYLIDSIAALASQPVVSPPPVVTAPVRQAPPLVSAPVVINEDPVRKTSSVPGPK
                     PDPKPVIVQSKPEPIPVVLPTIEEKFSSRQKVFIKEIPVAGDSVELRFYDNAEIDGDS
                     ISLFLNNKLLYQHIRLAAGAWT"
     gene            1170..3020
                     /locus_tag="PLU11_14320"
     CDS             1170..3020
                     /locus_tag="PLU11_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60358.1"
                     /translation="MKKIVPQCQLKGRPFSIGWSINEGVKRVFLLAVLFSLFQISYGQ
                     SGYEKPKVVVGELIKVIPSLRNFSADADTSMITRTTEGMVAKKTWTNPAFDFGTNAKG
                     DPVVQLNTKKLGSTATTSPNAGTEITQNFDGMGYTSVAPADPTMCAGPNHVIQMINGG
                     SGAYFKVWDRSGVQVVGQTYMDLLTGAAYSGAGDPVALYDQFSGRYMLTEFGFTGGVT
                     SYINTLIVAVSQTSDPAGGWYIYRFQDNSFFVDYPHWGVWPDAIYATSNDFNTTGTAY
                     LGSSIYALNKAKMLTGDVTAELVRVRLTSTDNKFISTAPVSISGPTPPTAGSPGMFVY
                     YNDDNYTASGTDVDSLGIITMQPNFTTPSSTVITFSQQMTTAPFKSRVCASRNCIASG
                     GAGYDALSDRIMNRVQYRNFGTHEALVLNHTVDANFPGTAKAGLRWYELRRSGGAWSI
                     FQQSTYAPDADHRWMGSVNINSKGQIALAYNHSGTGKYASIYFSGRNEADPPGTLAVN
                     DVLLHQGTAYGTFGNRWGDYNDLANDVVNDSLFWFTAMYGSGASSWSTRVSSFKIGLC
                     TPSVSLALTQAGTVSVPEGTDIVYTNTVSAVCDPINGYTLTDTLPTSVKV"
BASE COUNT          789 a          726 c          708 g          842 t
ORIGIN      
        1 atagtccaag caccagccgc cagcctgata tgctgataca ggagtttatt attcaggaaa
       61 agggaaatgg agtcgccgtc tatttccgcg ttatcataaa aacggagttc gacagagtca
      121 ccggctaccg gtatttcttt aataaatact ttttggcggc tgctgaattt ttcttcaata
      181 gtgggtaata caacaggtat gggttcaggt ttggactgaa caataaccgg ctttggatct
      241 ggtttgggcc cgggcaccga tgaagttttc cggaccggat cttcgttgat cacaacagga
      301 gcagatacaa gcggaggtgc ttgtctgaca ggagccgtaa ccaccggcgg tggtgaaaca
      361 acgggctgtg aagcaagggc ggcaatacta tcaatcaggt acggatcatt tccccctacc
      421 atataattat cgatgatata atcccactca tcggcaaaat cagtgtttcc ggctttatca
      481 agatctacct gccctttcgg atcattttca tcatccctgc gggtggcttt cccatccagc
      541 atcattttac ttcctcccgg acaattaaaa tcagcccgcg ccaggagggg cattttaccc
      601 ggactgctta ccgaaaaccg acccgctttt tcttccagca attgatcatc ccaccaaatg
      661 gcctcgttgg ttcccggatc gaaataacct ttaatgctat aacgacggta atgactggcg
      721 gattcgtagt aatacgaagt gcccgtcagg ctatcgccct tctgaatgat tttcacttcg
      781 actttctggc ggttgatccg gcccttccag acacccgtga tcatctggct gtggccaact
      841 agtgaaatac tgagtaaaag gaagctgagg aggggtcgta tgattatttt tttcaatttg
      901 gaagtttaat gctgtttttc cgggcatcac tcaaacgact gactgataac aaatattttg
      961 cctgaaattc aatgggatta gttttggctg gaatttggtt tatttactat attagtcccg
     1021 tattttgtgc caaaaccagc ttataaagga agtacttacg ctcctttcac aaagttattc
     1081 tacgctttgt gcgtattggt caaaatgccg gatagcctat caaaaccacc agttctacag
     1141 aacacacttt aaaccaaact tctgcaagta tgaaaaaaat tgtacctcag tgtcaattga
     1201 agggccgccc gttttccatt ggctggtcca tcaacgaggg cgtgaaaagg gttttccttt
     1261 tggctgttct cttcagtctt tttcagattt cttatggaca atcaggctat gaaaagccaa
     1321 aggttgtggt aggtgagtta atcaaggtga ttccttctct gaggaatttc tccgctgatg
     1381 ccgatacctc catgattacc cggaccactg agggtatggt tgccaaaaaa acctggacca
     1441 atccggcttt tgactttggc accaatgcca aaggagatcc cgtagtccag cttaatacaa
     1501 agaaactggg tagtaccgcc actaccagtc caaacgccgg tactgagatc actcagaact
     1561 ttgacggaat gggctatacc agtgtggctc ccgctgaccc caccatgtgt gcaggtccca
     1621 accatgttat tcaaatgatt aacggaggtt ccggtgctta ctttaaagtc tgggaccgca
     1681 gtggtgttca ggtggtagga caaacctata tggacctgtt aaccggcgca gcttatagcg
     1741 gtgccggcga ccccgtagct ttatatgatc agttcagcgg ccgctatatg ctgactgaat
     1801 ttggtttcac cggcggtgta accagctata tcaatactct gattgttgct gtatcgcaaa
     1861 ccagtgatcc ggcgggtggc tggtatattt atcgtttcca ggacaacagc ttctttgtgg
     1921 attacccaca ctggggtgtt tggccggatg cgatctatgc tacatccaac gactttaata
     1981 ctaccggtac tgcctatctc ggttcctcta tttatgcatt aaataaggcc aagatgctga
     2041 ccggtgatgt aacagcagaa ctggtacgtg tacgcctgac cagtacggat aataaattta
     2101 tttccacagc acctgttagt atcagcggac caacgcctcc cacggctggt tcaccgggta
     2161 tgtttgtgta ttataatgat gacaactaca cggcctctgg tacagatgtg gatagtctgg
     2221 gtatcattac catgcaaccc aacttcacaa cgccatccag tacggttatc actttttcac
     2281 agcaaatgac aaccgcccct tttaaatcca gggtctgcgc cagcagaaac tgtatcgctt
     2341 cagggggggc cggctatgac gccttaagtg accggatcat gaaccgggta caataccgga
     2401 atttcggcac acatgaagca ttggtattaa atcatacggt agatgcaaac ttccctggca
     2461 ccgctaaagc cggcttacgc tggtatgaac tcagaagaag cggcggagcc tggagcattt
     2521 tccagcagtc tacttacgca ccggatgctg atcaccgctg gatgggttct gtaaatatta
     2581 acagtaaagg acaaattgcc cttgcctata atcatagtgg taccggtaag tatgcctcaa
     2641 tttatttctc cggaagaaat gaagccgatc cccccggcac actggctgtc aatgatgttt
     2701 tattacatca gggaacagct tatggtacat ttggtaaccg ctggggtgac tataatgatc
     2761 tggcgaatga tgtggtgaat gactccctgt tctggtttac cgccatgtac ggaagtggcg
     2821 ccagcagttg gtccaccagg gtatcttcct tcaaaatcgg tctctgtacc ccaagtgtta
     2881 gtttagcact gactcaggca ggtacggtat cagtgcctga aggaactgat attgtttata
     2941 caaatacggt tagtgccgtt tgtgatccga tcaacggtta tactttaact gatacgctgc
     3001 ccacttcagt aaaagtataa ccgttgatcg gatcacaaac ggcactaacc gtatttgtat
     3061 aaaca
//
LOCUS       DAOPMW010000408         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_38887_length_3062_cov_6.337878, whole genome shotgun sequence.
ACCESSION   DAOPMW010000408 DAOPMW010000000
VERSION     DAOPMW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38887_length_3062_cov_6.337878"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLU11_14325"
     CDS             <1..773
                     /locus_tag="PLU11_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPN60359.1"
                     /translation="VLVILEVVLGIDNVIFVSIILNRLKGDKAQRRARRAWMVTGILM
                     RSILLMGLGWLLSQKGKAVFTLGDKGFDLASIVMLAGGLFLIYKSVMEIHHKLEGEDP
                     NESLRNQKPLGFGQAIGQIVLIDAVFSFDSIITAGGTAKHVEIMIAAVVIAMTIMFLF
                     SPAIAGFIHKHPTLKMLALSFLVMIGLSLIIEGWDSEAAHQLHLKNYIYFGMAFSFVV
                     EMLNMTMMGRAKKRKVHVVELNEPVLEEKKEDYNDQAK"
     gene            complement(834..2048)
                     /locus_tag="PLU11_14330"
     CDS             complement(834..2048)
                     /locus_tag="PLU11_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60360.1"
                     /translation="MKKVLIICLVFLASGILQAQPGKNTVLIVLQENTGKLSFAQSIP
                     EPFRSTLNNVVDGLAENFENFKAKLQLSDRYEKVILLTNTACTRANLLTNLINQTKLG
                     KTVDLLVLGHGGNTQSGNTFFLVLNNDERLPEDDVTLTIGKVNKSFKGIRSLITDARA
                     TQGSGFNFNLRLVYMCNCKGGNTNDAWLSIGAKTAVGTVNNNYMPEPQITYFFNDFVI
                     NGKTAQQAANDSWAASRPFYSLIPQMQNRENNLTLIDCSKMKVDGNASLRFAASKLGV
                     NESKSFVIKAGESFNHTTLFMSAGEQYQFTVGGTDKWKNGGTETTANGYQPGILDAAR
                     RQGSYNMMTLVGEIFRDLNNTSYTGTHFKIGTSRTYTAAISGYLMCHANDVLIGYGDN
                     TGLVTMTVKRLK"
     gene            complement(2082..>3062)
                     /locus_tag="PLU11_14335"
     CDS             complement(2082..>3062)
                     /locus_tag="PLU11_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60361.1"
                     /translation="QPFWELFYNRKDLSKYQAANGFMRKLASLDISMGTVQNEETDRR
                     IGMAAKLNLFKKKDPLMVKSMYEDIGIRFTEEKQTLETKLKELQFKLDTVQNIMDKPG
                     IRSEIKSTEEQLFSLNSRRNTEINDRAKIFIAENWNAASLDIAIGRVYTYQSDSAGSL
                     KKLRLNRNTGWGIWLNGALPLGKRWMFSGLVRTTWYEEELDFLLRNTSTLEERTAQAV
                     AENKIYSAGLNLRYGGPLYSFFVEFLYEKKALKTPVEALRDVFTEPANTVVVSNSVKW
                     TVVHPNSLSIGGDWRISQNVIINYGMRWILDVNGKSQAFIPVAGISCMMR"
BASE COUNT          784 a          724 c          673 g          881 t
ORIGIN      
        1 ttgtcctcgt catccttgaa gtggtgctgg gcatcgataa tgtaatattc gtcagtatca
       61 tcctgaaccg cctgaaaggc gataaagcac agcgaagagc ccgtcgtgcc tggatggtca
      121 ccggcatcct gatgcggagt atattactga tgggattggg atggttgctt tcgcaaaaag
      181 gaaaagctgt ttttaccctt ggcgacaaag gatttgatct cgcgagtata gtgatgcttg
      241 ccggcggact cttcctgatc tacaaatcag tgatggaaat acatcataaa ctggaggggg
      301 aagatccgaa tgaatcctta cgcaatcaga aaccacttgg ttttggccag gccatcggtc
      361 agattgtact catcgatgca gtgttctctt ttgacagtat cattacggcc ggtggtacgg
      421 ccaagcacgt agagatcatg atcgcagcag tagtgattgc catgacgatt atgtttttat
      481 tcagtcccgc cattgcgggc tttatccata agcaccccac gctgaaaatg ctggccctct
      541 ccttcctggt aatgattggc ttaagcctga tcattgaagg ctgggacagt gaagcggcac
      601 atcagctaca tctgaaaaat tatatctact tcggaatggc cttctccttt gtggtggaaa
      661 tgctgaatat gaccatgatg ggccgggcga aaaaacgaaa agtacatgtg gtggagctga
      721 atgaaccggt gctggaggag aaaaaagagg attacaacga ccaggcaaaa taatttaaaa
      781 ggcgcttctg cagaagcgcc ttttaaatta tcagatactt atagaacgat tttttacttc
      841 agtcgtttaa ccgtcatggt caccagtcct gtattatccc cataaccaat cagcacatca
      901 ttagcatgac acatcaggta acctgaaatg gctgccgtat aagtccggga agtgccaatt
      961 ttaaaatggg ttcctgtgta agaagtatta ttgagatccc tgaaaatctc ccccaccagg
     1021 gtcatcatat tataactgcc ctgacgacgg gcggcgtcga ggataccggg ttgataacca
     1081 ttggcggtag tttctgtacc cccatttttc catttatcgg ttcctccaac ggtgaactga
     1141 tactgttctc cggcactcat aaaaagggtg gtatggttaa aagattcacc cgcttttata
     1201 acaaaagatt ttgactcatt cacccctaat ttgctggcgg caaaacgcaa gctggcattt
     1261 ccatccactt tcattttgga acaatcaatc agggtcaggt tgttttccct attctgcatt
     1321 tgcggaatca gtgaataaaa cggacgggaa gcagcccagg aatcattagc agcctgctga
     1381 gccgttttcc cattaatcac aaagtcatta aaaaagtaag tgatctgggg ttcgggcata
     1441 taattattgt ttactgtacc cacggccgtt ttcgccccga tactcagcca ggcatcgttg
     1501 gtatttccac ctttgcaatt gcacatataa accagacgca gattaaaatt gaacccgctg
     1561 ccttgtgtgg ctcttgcatc tgttatcagg ctcctgattc ctttaaatga tttgttcact
     1621 ttcccaattg taagcgttac atcatcttcc ggaagtcgtt catcattatt cagcacaaga
     1681 aaaaaagtgt tcccgctctg tgtatttccg ccatgtccaa gcaccagcag gtctaccgtt
     1741 ttgcccaact tagtctgatt gatcagattc gttaacaggt tggccctggt gcaggctgta
     1801 ttcgtcagga ggatgacctt ttcatagcga tcactcaatt gcagctttgc tttaaagttc
     1861 tcaaaattct ctgccaatcc gtccactaca ttattaagcg tgctgcggaa tggttcaggt
     1921 atactttgag caaaactaag cttgcctgta ttttcctgca atacaatcaa tacggtgttt
     1981 tttccaggct gcgcctgcag gattcctgaa gccaggaata ctaaacagat aattaaaact
     2041 tttttcatcg gtgtttgttt tatggtttgt taattgattt attatctcat catacaggat
     2101 atccccgcca ccgggataaa ggcttgtgat tttccattga catccagtat ccagcgcatg
     2161 ccataattga tgatcacgtt ctggctgatc cgccagtcgc cgccgatact gagtgagttg
     2221 ggatgcacca ctgtccattt tactgaatta gaaacgacca ccgtattagc tggttcggta
     2281 aagacatcac gcagtgcttc gaccggtgtt ttgagggcct tcttctcgta gaggaattca
     2341 acaaaaaaac tatagagcgg gccgccataa cggagattaa gaccggcact gtatatttta
     2401 ttttcagcca ctgcctgtgc agtccgctct tccagtgtgc ttgtattgcg gagcagaaaa
     2461 tccaattcct cctcatacca ggtggtacgc accagcccgg aaaacatcca tcgtttaccc
     2521 aggggcaatg cgccattgag ccagatgccc cagccggtgt ttcgattcag tcgcaatttt
     2581 tttaaagaac cggcactatc actctgatac gtgtacacgc ggccaatcgc aatatcaagg
     2641 gaagccgcat tccagttctc cgcaatgaat atttttgcac ggtcattgat ttccgtgtta
     2701 cgccggctgt tcaggctgaa taactgctcc tccgtacttt tgatttcgga cctgataccc
     2761 ggtttatcca taatattctg aacggtatcc agcttaaact gcagttcctt cagtttggtt
     2821 tccagtgttt gtttctcttc agtaaagcgt atccctatat cctcgtacat ggattttacc
     2881 atgagcggat cttttttctt aaacaaattc agtttagcgg ccattccgat ccgccggtca
     2941 gtttcctcat tctggacggt ccccatggaa atatccaggg aggcgagctt gcgcataaaa
     3001 ccattggccg cctggtactt tgacagatcc ttccggttat aaaacaattc ccagaatggc
     3061 tg
//
LOCUS       DAOPMW010000409         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_38952_length_3059_cov_8.489680, whole genome shotgun sequence.
ACCESSION   DAOPMW010000409 DAOPMW010000000
VERSION     DAOPMW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38952_length_3059_cov_8.489680"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="bshA"
                     /locus_tag="PLU11_14340"
     CDS             <1..884
                     /gene="bshA"
                     /locus_tag="PLU11_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HPN60362.1"
                     /translation="IKNNDLDLLHVHYAIPHASAAYMAKQILKKEGKNIPVITTLHGT
                     DITLVGRDKTYAPVVTFSINESDAITAVSENLRMETLSHFHIEKEIDVILNFVDVSRF
                     NRKPIDAFRKVIAPNGERILLHASNFRKIKRVQDVVKIFAAVNKQIPSKLLFVGDGPE
                     RNTAEDLSRELGVCDEIRFVGKQEQMEDILAIADLFLLTSEYESFGLAALEAMAAGVP
                     VVSTNAGGLGEINIQGVTGFLSNVGDVEGMTNQSLAILKDDATLKGFKDRAAEHSKKF
                     DIHHIVPQYEKLYERFL"
     gene            complement(935..2275)
                     /locus_tag="PLU11_14345"
     CDS             complement(935..2275)
                     /locus_tag="PLU11_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPN60363.1"
                     /translation="MRKKILLPFFLFGFIAMGIAQPQDKTQLEKERQEIQRELQEIQG
                     MYNKVKGQTKQNLAQLNMLKRKIDLQEQYINSINKELRSIDDDIYLSELEIYRLNKQL
                     DTLKSQYAKSIVYSYKNRSNYDYVNFIFSASSFNDAVKRIAYLKSYRSYREKQVSTIQ
                     ETQQLIAQRKKQQLVRKDQKNVALQSQTKQVQELAVQKKEKDVVVTQLKSKEKELANQ
                     IASKKKRDRDLLNAVNAIVRREMEAAKAKAKADAEAKRKADEANAKSGDKTGNPVTSS
                     GNNAGTNPPVVTKRNEAVKKPDSYLDFNSKDVALNSSFQANKAKLPWPVDKGVITLRF
                     GTNKIENTLLTFDNPGLTIATSAAGVPVKAVFDGEVSGVYNLGDGMAVTIRHGKYFTT
                     YSNLAGVNVTKGAIVKTGQQIGKAGRDDDGSGGQIDFILMIESRNVDPAPWLRR"
     gene            complement(2355..>3059)
                     /locus_tag="PLU11_14350"
     CDS             complement(2355..>3059)
                     /locus_tag="PLU11_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPN60364.1"
                     /translation="PPLDTGHADSLTLIKEYYTAMINQRISFTTFSGKLDVDYEETGG
                     KKLNLNAELRMYKDSVIWIRITAILGIEGLRAYITKDSVKILNRQDKVYIARSVAFLQ
                     EIAALPLDLASLQDLLIGNPVFISSDISSYSKSSGNVSLHGLNSLFKTLITVGEMDKQ
                     LYSAKLDDLDELRNRTSYLTWSDYENKKGMPFAQKRSITVAEKKTLEIKLNYKQYEFN
                     ETLSFPFSVPKNYKAE"
BASE COUNT          749 a          691 c          701 g          918 t
ORIGIN      
        1 tgatcaagaa caatgacctg gacctgttgc acgtgcatta tgccatcccg catgcctctg
       61 ctgcttatat ggccaaacag atcttaaaga aagaaggaaa gaatattccg gtgatcacca
      121 ccttgcatgg aacggacatt acgttagtag gacgcgataa aacctatgct cccgtggtta
      181 ctttttccat caatgaaagt gatgccatca cggcggtatc ggaaaacctg agaatggaaa
      241 cactcagtca tttccatatt gaaaaagaga tcgatgtcat cctgaacttt gtggatgtga
      301 gtcgtttcaa ccgtaagccc attgatgcgt ttcgcaaagt gatcgccccg aatggagaac
      361 ggatcttatt gcacgcttcc aatttccgga agatcaaacg ggtacaggat gtggtgaaga
      421 tcttcgctgc tgtaaataaa cagatcccct ccaaattatt atttgttggt gacggacctg
      481 agcggaatac cgcggaagac ctgagtcgcg aactgggcgt atgtgatgaa atccggtttg
      541 taggtaaaca ggaacagatg gaagatattc tcgcgatagc tgatttattc ctgctgacat
      601 ctgaatatga aagctttggt ctggcggccc tggaagccat ggcggccggt gtgccggtgg
      661 tatccaccaa tgcaggtggt ctgggtgaaa taaatataca gggcgtaact ggcttcctca
      721 gcaatgtggg cgatgtggag ggtatgacca accagtccct ggcgatttta aaagatgatg
      781 ccaccctcaa agggttcaaa gaccgggccg cggaacattc gaagaaattt gacatccatc
      841 atatcgtacc gcagtacgaa aaattatatg aacgcttttt ataaataaaa aatcccggca
      901 aaaccgggat tttttgttga atatcatttt cagtttaacg gcgcaaccag ggtgccggat
      961 ccacattacg ggattcgatc atcaggataa aatcaatctg tcctccgctg ccgtcatcat
     1021 cccttccggc tttcccgatt tgctgtcctg tttttacaat tgcccctttc gttacattca
     1081 caccggctaa attactgtac gtagtaaagt atttaccatg ccggatggtg acggccattc
     1141 cgtcccctaa attatagaca ccactcactt cgccgtcaaa taccgctttt accggtacac
     1201 cagctgcaga agtggcaatg gtgaggcctg gattatcaaa cgtgagcagg gtgttttcaa
     1261 ttttattagt cccaaagcgc agggtgatca cgcctttatc cacgggccag ggaagcttgg
     1321 ctttattggc ctggaaactg ctgttgaggg ccacgtcttt tgaattaaaa tcgaggtagc
     1381 tgtcgggctt tttgaccgct tcattccgtt tggtaactac tggtggattt gtacctgcat
     1441 tgttccccga agaagtaacc gggttgcctg ttttatcccc gctttttgca ttcgcttcat
     1501 cggctttccg ttttgcttcc gcatccgctt ttgccttggc tttagcggct tccatttccc
     1561 ttcttacaat ggcgtttacc gcattcaaca ggtcacggtc ccgtttcttt ttactggcaa
     1621 tctggttggc cagttctttt tctttggatt tcaattgggt gaccactacg tctttttcct
     1681 ttttctgcac ggcgagctcc tgcacctgtt tagtctgact ctgcagggct acattcttct
     1741 ggtctttgcg taccagttgc tgctttttcc gctgggcaat cagctgctgg gtttcctgga
     1801 tggtggaaac ctgtttctcc cggtaagaac gatagctctt cagataagcg atccgcttga
     1861 ccgcatcatt gaagctactg gcagagaaaa tgaaattgac atagtcgtag ttactcctgt
     1921 ttttatagga gtatacaatc gatttcgcgt actgggattt aagggtgtcc agttgcttat
     1981 tgagacggta aatctcgagt tcgctcaggt aaatatcatc atcgatactg cgaagttcct
     2041 tgttgataga attgatatat tgttcctgca ggtcgatctt ccttttgagc atgttcagct
     2101 gggccagatt ctgtttggtc tgtcctttta ctttattata cataccctgg atctcctgca
     2161 gttcccgctg gatctcctgt ctttcttttt ccaactgtgt tttatcctgt ggctgggcaa
     2221 tacccatggc tataaatccg aacaggaaaa aaggcaacaa gatttttttt ctcatgatta
     2281 actggattga ggttaaaaaa acagggtcgg aatcagttat ggaaaacggc agactgcaaa
     2341 ataaagtata tcctttattc ggctttatag tttttaggca cggaaaaagg gaaacttaac
     2401 gtttcattaa attcatactg tttataattc agtttgattt ccagcgtttt cttttctgct
     2461 accgtgatcg accgtttctg ggcgaagggc atccctttct tattttcata atcactccag
     2521 gtcaggtaac tggtccggtt gcgcaactca tccaggtcat cgagcttggc actgtagagt
     2581 tgtttatcca tttcccccac ggtgatcagg gttttgaaca gactgtttaa cccatgaagt
     2641 gaaacattgc ctgatgattt gctataagaa gaaatatcgg aactgataaa taccgggttg
     2701 ccgatcagga ggtcctgcag ggaggccagg tccaatggca gggcggcgat ttcctggaga
     2761 aaagccacac ttcgggcgat atacacttta tcctgccggt taagaatttt tacggagtcc
     2821 tttgtgatat aggctctcaa tccttcaatg ccgaggatag cggtaatacg gatccagatc
     2881 acactgtctt tgtacatcct taattctgca ttcaggttta attttttacc gccggtttct
     2941 tcataatcca cgtccagttt accagagaaa gtggtaaagc tgatccgctg attgatcatc
     3001 gcagtataat actctttaat cagcgtcaat gaatcggcat gaccggtatc caatggagg
//
LOCUS       DAOPMW010000410         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_39204_length_3046_cov_4.161819, whole genome shotgun sequence.
ACCESSION   DAOPMW010000410 DAOPMW010000000
VERSION     DAOPMW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39204_length_3046_cov_4.161819"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLU11_14355"
     CDS             <1..364
                     /locus_tag="PLU11_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPN60365.1"
                     /translation="ASDSGFYIGYRSVFDKNDSIHFHYVAQQKSLLQPVADHEDLQHL
                     IRFSKGYYTVENRNDTLLFNDIRFGEQIGWRKSGAPFVFYYYLQHPDENKMIVQRGRF
                     AGWDRSAFSSLLRRIKRE"
     gene            complement(365..1543)
                     /locus_tag="PLU11_14360"
     CDS             complement(365..1543)
                     /locus_tag="PLU11_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPN60366.1"
                     /translation="MSQIKKLIHMGYTSRNRVLMVQGGRDFFSRLNDLIEAASHSIHL
                     QFYIFLDDETGNAVIRSLQTAASRGVAVYLQLDAYSSRELSQKSVDALRAAGVQVKWF
                     EPLFKSRHFYFGRRLHHKVVVVDGYRTLVGGINICDRYNDLPGDPAWLDMAVYCEGEA
                     AYITYLICRRMWQDRINQPALPWKDIEAACDTIPEKERTEVRVRQNDWVKRKVQIWSS
                     YLEAFNRSEKELTIMCSYFIPGILYRRKLAQAVKKGVRVKVILAGISDIKMAKHAERF
                     LYEWMLKNKISIYEYQETVLHAKVATYDGSWTTVGSYNVNNISAYASLELNLDIRNKD
                     FATATSAMMDNIIRDHCKEITRENYINKVNIFVRFWQRISYGFFNTVLKLFTFYFKQE
                     "
     gene            1587..1967
                     /locus_tag="PLU11_14365"
     CDS             1587..1967
                     /locus_tag="PLU11_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60367.1"
                     /translation="MSSIICGFDATHLYRLLQGATFKKKDHYYDCNSAAGNSPLPEHC
                     SLDNSGFTTVFRVYIDKEKGYSVFTNVSVSLFYTGSGLLILRIALNTQTGVRMAPVFC
                     GLASFNKSHLYEKDAAFILFQLVW"
     gene            1924..>3046
                     /locus_tag="PLU11_14370"
     CDS             1924..>3046
                     /locus_tag="PLU11_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPN60368.1"
                     /translation="MKKMLLLFYFSWFGNGHYQVLHTQENEPLYYPLLVEQFYQGNQE
                     QLFWFAPGTEATALRMSLSGLNQTALQQGLAARKYHAVIIENGSVPEYADSMALKQQD
                     RYFTDAALSLMFDIFQGVKERPWVGFDAVSEKYRQSDGEKMIQCLLRAKTAEELVITL
                     DLLEPKDTVYAALKNEYQRLLLANRKDSARLVRLSMNYYRWITHFHFDKMIVVNLSAA
                     RLHYYENNQPILQMKTIVGKTSTPTPRFAAWCDKAILYPYWYVPRSITFNEYLPVIRR
                     NPSWLDAKNMQVIDGKGRVVNHHLLDWSSFHAGYFPYTIRQSTGCDNALGVIKFNINT
                     PYGVYLHDTNNKHVFSSGNRYLSHGCIRQRKHAYYLYHAG"
BASE COUNT          778 a          705 c          697 g          866 t
ORIGIN      
        1 cgcatccgac tccggttttt atatcgggta ccgctcggtt tttgataaaa atgacagtat
       61 ccactttcat tatgtggctc agcaaaaatc actgctgcag ccggtggcgg atcatgagga
      121 cctgcagcac cttatccgtt tctccaaagg gtattatacc gtagagaacc ggaatgatac
      181 tttgctgttt aatgatatcc gttttggcga acagatcggc tggcgcaaat ccggtgcccc
      241 ttttgtcttt tattattacc tgcaacatcc ggatgaaaat aaaatgatcg tgcaacgggg
      301 acgttttgcc ggatgggatc gctccgcctt tagttcctta ctccggcgta ttaaaaggga
      361 gtaattattc ctgtttaaaa tagaacgtaa ataatttgag cacggtatta aagaagccat
      421 aactgatccg ttgccagaaa cgtacgaata tatttacttt gttgatgtaa ttctccctgg
      481 tgatttcctt acaatgatcc ctgatgatgt tatccatcat ggcacttgta gctgtggcaa
      541 aatctttatt ccggatatcg aggtttaatt caaggctggc ataggcactg atgttattta
      601 cattgtaaga ccctaccgtg gtccaggatc catcataagt ggccactttt gcatgcagga
      661 ctgtttcctg gtattcataa atggagattt tgtttttcag catccattcg tacaggaaac
      721 gctcggcatg tttggccatt tttatatccg aaatgccagc gagaattact ttcacccgga
      781 cccctttctt tacagcctga gccagtttac gccggtacag gataccagga ataaaataac
      841 tgcacatgat cgtcagttct ttttctgacc ggttgaaagc ttccaggtaa gaactccata
      901 tctgaacctt tcgttttacc cagtcgtttt gccgcacccg cacttctgtc cgttcctttt
      961 ccggtatggt atcacaagcc gcttcgatgt ccttccaggg cagggcgggc tgattgattc
     1021 gatcctgcca catccggcgg cagatcaggt aagtaatata agccgcttct ccttcacaat
     1081 aaacggccat atccagccag gcaggatcgc ctggaagatc attgtaccgg tcgcaaatat
     1141 taatgccgcc aaccaacgtg cggtaaccat ccaccaccac tactttatga tgcagcctgc
     1201 gcccaaagta aaagtgcctg cttttgaaca agggttcaaa ccattttacc tgaacaccgg
     1261 cagcccgtaa cgcatccact gatttctgag atagttcccg ggaagagtag gcgtccagtt
     1321 gcaggtaaac cgccactccc cgggaagcgg cagtctgcag cgatcggatg acggcgttac
     1381 ccgtttcatc gtccaggaat atataaaact gcagatggat ggaatgactg gccgcttcaa
     1441 tcaggtcatt caaccggcta aaaaaatccc ggcctccctg taccattaat acccggttgc
     1501 ggctggtata gcccatatgg atcagttttt ttatctggct cattttgaat ctgggagggc
     1561 aattgcctgt ctgtgcaaaa ttacgtatga gttcaatcat ctgcgggttt gatgcaactc
     1621 atttataccg gctccttcag ggggctacct ttaagaaaaa ggatcattat tatgattgca
     1681 attccgccgc cggaaacagc cctttacctg agcattgcag cctggataac agcggtttca
     1741 ctactgtatt cagggtttat attgataaag aaaaagggta ttccgtcttt actaatgtgt
     1801 ctgtatcgct tttttatacg ggaagcggcc ttttaatcct gcgaattgca ttgaatacac
     1861 aaaccggggt caggatggcc ccggtttttt gtggtcttgc ctcttttaat aaaagccatt
     1921 tatatgaaaa agatgctgct tttattttat ttcagctggt ttggtaacgg acattatcaa
     1981 gtcctgcata cgcaggaaaa tgaaccgcta tattatcccc ttctggtcga acaattttac
     2041 caggggaacc aggagcagtt attctggttt gcacccggta ctgaagcaac tgccttacgg
     2101 atgagtttat ccgggctgaa ccaaacagct cttcagcagg gcctggctgc gaggaagtat
     2161 catgctgtga tcattgaaaa cgggtcagta ccggagtacg ctgattcgat ggctttgaag
     2221 caacaggacc ggtattttac ggatgccgca ctctcgctga tgttcgatat tttccaggga
     2281 gtgaaagaac ggccatgggt tggttttgat gcggtatcag aaaagtacag gcaatccgat
     2341 ggagagaaaa tgatccaatg ccttttgcgg gcaaaaacag cggaggagct ggtcattact
     2401 ttggatctgc ttgagccgaa ggatacggtt tatgcggccc tgaaaaatga atatcagcgt
     2461 ctgctgttgg cgaaccggaa agattccgcc cggttggtcc ggctcagcat gaattattac
     2521 cggtggatta cacattttca ttttgataaa atgatcgtcg ttaacctgtc tgcggcccgg
     2581 cttcattatt atgaaaataa tcaaccgata ctccagatga aaacaattgt aggaaaaacg
     2641 tccaccccca caccgcgctt tgccgcgtgg tgcgataagg ccattcttta tccgtattgg
     2701 tatgttccac gaagtattac tttcaatgaa tacctgccgg taatccggag aaacccttcc
     2761 tggctcgatg caaaaaatat gcaggtgatt gatgggaagg gccgggtggt caatcatcac
     2821 ctgctcgact ggtcttcttt tcatgccggg tattttccgt atactatccg tcaatccacg
     2881 ggatgtgata atgcactcgg ggtcataaag tttaatatca atacgccgta tggtgtttac
     2941 ctgcatgata caaataataa gcatgttttc tcttccggta accgctacct cagccacgga
     3001 tgtatccggc agagaaaaca tgcttattat ttgtatcatg caggta
//
LOCUS       DAOPMW010000411         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_39565_length_3027_cov_8.299462, whole genome shotgun sequence.
ACCESSION   DAOPMW010000411 DAOPMW010000000
VERSION     DAOPMW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39565_length_3027_cov_8.299462"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLU11_14375"
     CDS             complement(<1..266)
                     /locus_tag="PLU11_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN60369.1"
                     /translation="MKRRTMLRQLSMAVPAMYMGSQISPEWLTAFPGPGEPMAKGPFA
                     PDWKSLQQYQVPDWFRDAKFGIWAHWGAQCQPEYGDWYARSMYI"
     gene            complement(278..2038)
                     /locus_tag="PLU11_14380"
     CDS             complement(278..2038)
                     /locus_tag="PLU11_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60370.1"
                     /translation="MDRASFLRMSTLAGLAPLCTPLLSFAGSNNGTEDAALIARLLAA
                     NDKLVKGLLETVQEGKLNFSRKTAYDFALLSGSYCEKSSVYYQDPRIMSKLGILARFL
                     TDSQSADGTVNVGNLESPPDTAFVIEIVSTATAALQQQTVPELKPVLEQIKTMILKAG
                     EALRTGGIHTPNHRWVICAALSQIHALYPDKKYVKRINEWLAEGIYQDADGYYPERSG
                     TYSMVENTAFITMARNLKRPDLLKYVRKNLTTFFYSMEANGDLVSNNSRRQDQFLCRH
                     STIFYLQYRYLAIHDQDRHFAAICKMIEATPWFEKEVLQQGLFYFQENETIRTSLPSP
                     ATLPDRYEKLFPSSHLLRIKDKQTYITLFGGADWPLIIASGRACSPDIFSYRKGKAVL
                     KYLRLSTTFFSMGYFYSEGLKKQDNSYVLHKKLQIPYYQPLPLNKRNKQGDYKLSPSV
                     DDRFWNKMDFANRPQSNIKTLDTSIIMTEKNGEVELAFSITGQQKVPVTIELCFNEGG
                     QFTGLTTDSKGNAILAGDKAIYEVEGEQLHFGPGSFTNAVPENLEGERYSTHFGTLKT
                     SGTRVYLTGLTPFKHTLFIK"
     gene            complement(2056..>3027)
                     /locus_tag="PLU11_14385"
     CDS             complement(2056..>3027)
                     /locus_tag="PLU11_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HPN60371.1"
                     /translation="LGVLRKEAPEHFHMILDESNPNYMDLPGLSVLIGGMLINNLAYW
                     GCNQYIVQRALGADLKTARNGILFAAFLKLLIPVIAVLPGIIMYVLHKNGEFQQEMVD
                     GTVLKPDHAYPTLMNLLPAGLKGVAFAALTAAIVASLAGKANSISTIWSLDIYKKYFN
                     KEAPDRKVVRIGRWAVIISMVIAALITPALRSLDQAYQFIQEYAGFLSPGVLAIFLLG
                     MFWKRTTAAAALVGAVITIPVSTVLKFLPSWTGGAFPDYPFLDRMTITFFTVALVMIL
                     ISLVWKKSEKDRHTIEVDKAMFKVNPSFVIGSVIICGILAALYTIFW"
BASE COUNT          773 a          727 c          724 g          803 t
ORIGIN      
        1 tcgatataca tactgcgggc ataccagtca ccatactccg gctggcactg tgcaccccag
       61 tgggcccaga tcccgaactt ggcatcccgg aaccaatcag gtacctggta ctgttgcagg
      121 cttttccaat cgggagcaaa aggccctttg gccatcggct cacccggacc cggaaatgcg
      181 gttaaccatt ccggtgatat ttggctgccc atatacattg ccggcaccgc catggatagc
      241 tggcggagca tggttcttct tttcatgtct ggttcattta ttttatgaat aatgtgtgtt
      301 taaaaggagt taatcccgta agatacactc ttgtaccgct tgtctttaag gttccgaaat
      361 gtgtactgta ccgttcgcct tccaggtttt ccggaacggc gttggtaaag gaaccgggac
      421 cgaaatgcaa ctgctcccct tccacttcgt atatggcctt atccccggcg agtatagcat
      481 tgcccttact gtcggtggtc agcccggtga actgtccgcc ttcattaaaa cagagttcga
      541 ttgtcaccgg cactttctgt tgcccggtta tactgaaggc cagttctact tccccattct
      601 tctcggtcat gatgatgctg gtgtcgagcg tcttgatatt gctctgcgga cgattggcaa
      661 aatccatttt attccagaaa cgatcatcca cgctcggact caatttataa tcgccttgtt
      721 tattgcgttt attgagaggt aatggttggt aatacgggat ctgtagtttt ttgtgcagta
      781 cataactatt gtcttgcttc ttcagtccct cactatagaa ataacccatg ctgaagaatg
      841 tcgtggacaa gcgcaggtat ttaagcaccg ctttgccttt gcggtaacta aagatatccg
      901 gactgcaggc acggcccgaa gcgatgatca ggggccagtc tgctccacca aagagcgtga
      961 tataggtttg tttgtctttg atacgtaaca ggtgtgatga aggaaataat ttttcatagc
     1021 gatcgggtaa tgtggctgga gaaggcaggc ttgtccggat cgtttcattt tcctgaaaat
     1081 aaaacaagcc ctgttgcaat acttctttct caaaccaggg tgttgcttcg atcattttac
     1141 agatcgcggc aaaatgacgg tcctgatcat gaatggctag ataacggtat tgcaggtaaa
     1201 agatcgtgga gtgacggcag aggaactgat cctgtctgcg tgaattgtta ctcaccaggt
     1261 cgccattggc ctccatactg tagaaaaaag tagtaaggtt cttccgcaca tatttcagta
     1321 aatcgggtcg cttcaggttg cgggccatgg taataaaggc cgtattctcc accatggaat
     1381 acgttccgct acgctccgga taatagccat cggcatcctg gtagatccct tcagccagcc
     1441 attcattgat acgctttaca tattttttat caggatacaa tgcatgaatc tgcgataagg
     1501 ccgcgcagat gacccaacgg tgattaggag tatggatacc tccggtacga agtgcctcgc
     1561 ccgctttcag gatcattgtc ttgatctgtt ccagtaccgg cttcagttca ggcacggttt
     1621 gttgttgtaa ggctgctgta gcggtggata cgatttcaat cacgaaggcc gtatccgggg
     1681 gtgattcaag attaccgaca tttaccgtgc catcggcgct ttgtgaatct gtcaggaaac
     1741 gggcaagtat accaagtttt gacatgattc ggggatcctg gtaataaacg gagcttttct
     1801 cacaatatga accagataat agcgcaaagt cataggcggt cttccggctg aaattcagtt
     1861 taccttcctg aaccgtttcg agcagcccct tcaccaattt atcattggct gccaacagac
     1921 gtgcgatcaa agctgcatct tccgtaccgt tattacttcc cgcaaacgaa agtaaaggag
     1981 tgcagagcgg ggccagtcct gccaatgtac tcatccgtaa aaatgatgcc ctgtccatat
     2041 agtcagtttt ttatactacc aaaaaatcgt atacaaagcg gccagtatac cacagataat
     2101 caccgatccg atcacaaagg acggatttac tttgaacatg gctttatcta cttcaatggt
     2161 atgtctgtct ttttcacttt ttttccatac caggctgatc aggatcatta ccagcgcaac
     2221 ggtgaaaaaa gtaatcgtca tgcgatcgag gaagggataa tcagggaatg caccgcccgt
     2281 ccatgaagga agaaacttca gcactgttga aaccgggatc gtgatcacag ccccaacaag
     2341 cgccgctgcg gcggtagtgc gtttccagaa cattcccagc aggaagatcg cgagtacacc
     2401 aggcgaaagg aagccggcat attcctggat aaactgatag gcctgatcca gtgagcggag
     2461 tgccggtgtg atcagcgcag cgatcaccat agaaatgatc actgcccagc gaccgatacg
     2521 gaccactttg cggtcgggtg cttctttatt aaagtacttc ttataaatat cgagcgacca
     2581 gatcgtggag atgctgttgg ctttacctgc aagtgaggca acaatcgctg ccgttaatgc
     2641 agcaaaagcc acgcctttca atccggcggg taagaggttc attagtgttg gataagcatg
     2701 atcaggcttg agcacggtgc catccaccat ctcctgctga aactcaccgt tcttatggag
     2761 tacatacata atgataccag gtaatacggc aattacagga atcagtaatt tcaggaaagc
     2821 ggcaaaaaga ataccattac gggcggtttt aagatcagcc ccaagggccc gttgtacaat
     2881 atactgatta catccccagt aggccaggtt attgatgagc ataccgccga tcaggaccga
     2941 caacccgggc aggtccatat agtttggatt ggattcatcc aggatcatat gaaaatgttc
     3001 cggtgcttct ttacgcagca cgcccaa
//
LOCUS       DAOPMW010000412         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_39599_length_3026_cov_4.316728, whole genome shotgun sequence.
ACCESSION   DAOPMW010000412 DAOPMW010000000
VERSION     DAOPMW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39599_length_3026_cov_4.316728"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PLU11_14390"
     CDS             complement(<1..1335)
                     /locus_tag="PLU11_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN60372.1"
                     /translation="MIARIFLSALISVTLLQCIEAQTPVNDWENPLLYEVNKEAPHAS
                     FMLYADRASVLKDDFTKSPFYQSLNGSWKFNYTAKVANRIRDFYKPGLNTSQWKDIPV
                     PSNWELQGYGVPIYTNITYPFPKNPPYVGEDNPVGTYRKEFTVPASWNGKEIILHFGS
                     ITGCAFVYVNGQKVGMSKVSKTAAEFNITSLIKPGKNLLAVQVIRWHDGSYLEDQDFW
                     RISGIERDVFLFAMPKLTIWDFFIKSGLDAQYKDGVFSAEISLRSFKGNVQQKGKMEV
                     TVLGADGKTVFSLEKAIVKGTDSIQQILFNGVVNTPLKWSAESPNLYQCVIVLKDESG
                     KVVAQTGTRIGFRQVEIKNAQLLVNGVRVMVHGTNRHEHDDVLGHVPTLALMKKDIQL
                     MKQFNINAVRTSHYPNDPLWYKLCDEYGLYLVDEANIESHGMGAEKQARFNPG"
     gene            complement(1368..2663)
                     /locus_tag="PLU11_14395"
     CDS             complement(1368..2663)
                     /locus_tag="PLU11_14395"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HPN60373.1"
                     /translation="MLLACTLYGQQHGIPVLTRIINVGEGWAGNSVNTVVFRKNSIAS
                     LDSFQVISYYDADGFIVLGKRKLKDSLWEVKRTRYKGTITDAHRSISIALDGQGYLHM
                     AWDQHGNPLRYCRSISPFSLELGPELPMTGKKEGKVTYPEFYRMPDGSLLFFYRDGSS
                     GNGNLVINKYHLAEQNWSVLQDNLIDGEKSRNAYWQACVDSKGYIHISWVWRESPDVA
                     SNHDLCYAVSKDGGNTWEKSTAEKYTIPVTLATAEIICAIPQRSELINQTSMFADGSG
                     NVFIATYHKGRGNVPQYQLVYRIGNRWKRADLGFRKTGFSLSGQGTKKIPVSRPQVIS
                     WKTGRKTFAALIFRDEERGALVSLASAELKRNINWRIADLPTGTTGDWEPSYDSDLWK
                     EEKVLHLFMQPVIQADAEGVVKAAPSMVKVLECRVAGQK"
     gene            complement(2704..>3026)
                     /locus_tag="PLU11_14400"
     CDS             complement(2704..>3026)
                     /locus_tag="PLU11_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPN60374.1"
                     /translation="QKSLSSQDFTKTCLEIPVHYQATLIPSGCCGMAGAFGYEKEHYV
                     VSQQVGELVLFPAIRSAAPETIIAAAGTSCRHQVKDGTGRLSQHPVEILYEALKNDLT
                     ESRQ"
BASE COUNT          751 a          802 c          623 g          850 t
ORIGIN      
        1 acccggattg aaacgggctt gtttctccgc tcccatccca tgactttcaa tattggcttc
       61 atccaccagg tacagcccgt attcatcaca gagtttatac caaagcggat cattgggata
      121 atgggaagta cgtacggcat tgatgttaaa ctgcttcatc agttgaatat ctttcttcat
      181 cagggccagt gtaggaacgt gcccgagtac atcatcatgc tcatgacggt tggtgccatg
      241 caccatcacc cgcactccat tgaccagcaa ctgggcattt ttaatttcca cctggcggaa
      301 cccaatcctc gtacccgttt gcgccaccac tttgccagat tcatctttca aaacaatcac
      361 acattggtag aggttgggcg attcagcact ccatttcagg ggagtattga ccaccccgtt
      421 aaatagaatc tgctgaatac tatctgtccc tttcacaatt gctttttcca ggctgaatac
      481 cgtcttaccg tcagcaccca gcacggtcac ttccattttt cctttttgct gtacatttcc
      541 tttaaacgaa cggaggctga tctcagcact aaaaaccccg tccttgtatt gtgcatcaag
      601 tccggacttg ataaagaagt cccagatcgt cagtttcggc atggcaaaca gaaacacatc
      661 ccgctcaata ccactgatgc gccagaaatc ctggtcttcc aggtaactgc catcgtgcca
      721 gcggatcacc tgaacagcca gcaggttttt tcccggcttt atcaatgaag taatattaaa
      781 ctccgcagcg gttttggaaa ctttgctcat acccactttc tgtccattca catatacaaa
      841 agcacatccg gtgattgatc cgaaatgcag aataatctcc ttcccattcc aggatgcagg
      901 taccgtaaac tccttacgat aggtacccac cggattatcc tcgccaacat aaggcggatt
      961 cttggggaaa ggatacgtga tattggtata gatcggcacc ccgtatccct gcagctccca
     1021 attggatggt accggaatat ctttccattg actggtattc agtccgggct tataaaagtc
     1081 cctgatacga ttcgccactt tcgcggtata attgaacttc cagctgccat tgagtgattg
     1141 ataaaaaggc gacttcgtaa aatcatcctt taacacggag gctctatcgg cgtataacat
     1201 aaatgaagca tggggggcct ccttattgac ttcatataaa agcgggtttt cccagtcatt
     1261 cacaggagtt tgcgcctcta tacattgcaa taatgttaca ctgatcagtg cacttagaaa
     1321 aattctcgct atcataacgg atctgaatta atattacagt agttgttcta tttctgaccg
     1381 gcaacccggc attcaagtac tttaaccatc gaaggggctg cttttactac tccttccgca
     1441 tctgcttgta tcaccggttg catgaacagg tgcagtacct tttcctcttt ccacaagtcc
     1501 gaatcataag atggttccca atccccggtg gtgcccgtgg gaagatcggc aatcctccag
     1561 ttgatgttcc ttttcagttc ggctgatgca agggatacca aggcccctct ttcttcatcc
     1621 ctgaatataa gggcagcaaa ggtctttctt cccgtcttcc atgaaatgac ctgcggcctg
     1681 gaaaccggga ttttttttgt accctggccg ctgagtgaaa aaccggtctt cctgaaaccc
     1741 aggtctgccc gtttccacct gttaccgatg cggtaaacga gctggtattg tggcacatta
     1801 ccacgtccct tgtggtaagt ggcaataaaa acattcccgc taccatccgc aaacatagaa
     1861 gtctgattga tcagttcact ccgttgcggt attgcacaaa tgatctcggc agttgccagg
     1921 gtgaccggta ttgtatattt ctcagcagtt gatttttccc aggtgtttcc cccgtcttta
     1981 gataccgcgt aacagagatc atgattactg gctacatccg ggctttctct ccaaacccag
     2041 gagatatgaa tatatccctt actatccaca catgcctgcc agtaagcgtt gcggcttttt
     2101 tcaccatcaa taagattatc ctgtaatacg gaccagtttt gctcagccag gtgatatttg
     2161 ttgatgacca gatttccgtt gcccgaactt ccatcccggt aaaaaaataa aaggcttcca
     2221 tctggcatcc ggtaaaattc aggataggtc actttcccct ccttcttgcc ggtcattggc
     2281 aattcgggcc ctaattcaag ggagaaggga cttatactcc tgcaataccg aagcgggttt
     2341 ccatgctgat cccaggccat atgcaggtat ccctgtccgt ccagcgcgat actgatgctc
     2401 cggtgtgcat ccgtaatggt gcccttatac cgggttcttt ttacttccca aaggctgtcc
     2461 ttcagctttc gctttcccaa cacgatgaat ccatccgcat cataataact gattacctgg
     2521 aaactgtcca ggctggcaat cgaatttttc ctgaatacaa cggtattgac agaattaccc
     2581 gcccagcctt ccccgacatt tataatccgg gtgagtacag gtattccatg ctgctgtccg
     2641 tacagggtac aggccagcag cattaaaagg cttcctatga aatatttttt tatatacacc
     2701 ggactattgc ctgctttctg ttaaatcgtt cttaagtgcc tcatacaaaa tttcaaccgg
     2761 atgctgactt aaccttcctg taccgtcttt tacctggtgc cggcaggatg tacccgccgc
     2821 cgcaataatt gtttcagggg cagcggatct tattgcaggg aataaaacca gctcacccac
     2881 ctgttgcgac actacatagt gttctttttc ataaccaaat gccccggcca tgccacagca
     2941 ccccgaagga atcagcgtag cctggtaatg gaccggtatc tccagacagg ttttggtaaa
     3001 atcctgggag gaaagtgatt tctggt
//
LOCUS       DAOPMW010000413         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_40538_length_2976_cov_7.494694, whole genome shotgun sequence.
ACCESSION   DAOPMW010000413 DAOPMW010000000
VERSION     DAOPMW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40538_length_2976_cov_7.494694"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLU11_14405"
     CDS             complement(<1..817)
                     /locus_tag="PLU11_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN60375.1"
                     /translation="MKSRRQFIKNSSLTAAGLYLGAMSMSAKSYGRILGANDRVRLGA
                     IGFSDRFRQALFPSFLNHYKELNFDFVAVSDIWKVRREEGEAFLKNAMGHDITACRNN
                     EELYALKDVDAVIIATADFQHALHCIEAVKAKKDAYVEKPFAETMDDNIAAFKAVKAS
                     SQIVQIGSQRRSGGNYTAAADYIQSGKFGPITMVELTWNVNQPGRWRRPALVEICKEE
                     DTDWKRFLLNRPFEKWDPRKYLEYRLFWPYSSGMPGQWMSHQIDTVHWFSGLTH"
     gene            complement(926..2293)
                     /locus_tag="PLU11_14410"
     CDS             complement(926..2293)
                     /locus_tag="PLU11_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN60376.1"
                     /translation="MKRFRYFCLPLLLVSFITVNSQNGWAPLGNGKDLTGWKILGGKA
                     TYKVEDGAIVGTTVANTRNTFLCTEKEYGDFILELDVWVEDEEGNSGIQTRSHFDPAG
                     NNGEGKVYGRQCEVDPTWRRWTGGVYDEGRREWLYPMQLNARAQYAYKPNSFNHYRIE
                     CIGNEMKTWVNGIATAYVVDTLDSKGFIGLQVHAIGNPTHAGKKVVFKNLRIKTTGLT
                     PSAFPPGVYVVNLKPNTLTAYEQKDDWSLLFDGKSSKGWISAKGTAFPSTGWDISGGT
                     MHVLSSEGKEAANGGDIITQEQYRAFDLSFEFKLTPGANSGVKYFVTLNEKTSGSAIG
                     PEYQVLDDTLHPDAKLGRDGNRTLASLYDLIKADKQKRFLRPIGAWNLGRVVVYPNNK
                     VEHYLNGVKVLEYIRGSKEFRDLVAISKYVVWKNFGEAEKGHILLQDHGNEVWYRSVK
                     IKKLK"
     gene            2483..2938
                     /locus_tag="PLU11_14415"
     CDS             2483..2938
                     /locus_tag="PLU11_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60377.1"
                     /translation="MFTCTRTDSTNTDFKMLVNQLDRYLMGINGDDHDFFDQFNKIDN
                     IRHVVVLYLDGQPAGCGAIKEYEPGVMEVKRMFVPPAARGKGLATAILKELEVWAAEL
                     GYEKCILETGDIMPDAIALYKKNGYRSIPNYGQYVDVASSVCFEKHLAG"
BASE COUNT          770 a          762 c          624 g          820 t
ORIGIN      
        1 gatgtgtaag accgctgaac caatgcaccg tatcgatctg gtgactcatc cactgcccgg
       61 gcataccgga agaataaggc cagaacaaac gatactctaa atatttacgg ggatcccatt
      121 tttcaaaagg gcgattcaat aaaaaccgtt tccaatcggt atcctcttcc ttacagattt
      181 ccaccagggc gggtcttctc cagcgaccgg gttgatttac attccaggtg agttccacca
      241 tggtgatggg tccgaatttg cctgactgga tgtaatccgc cgctgccgta taattccccc
      301 cactccttcg ctgtgatccg atctgtacga tctgtgatga ggctttcacc gctttgaagg
      361 ctgcaatatt atcatccatg gtttcggcaa aaggcttttc cacgtaagca tctttctttg
      421 ctttcaccgc ttcaatacaa tgcagcgcat gttgaaaatc ggccgtcgca ataatcaccg
      481 catctacatc cttcaatgca tacagttcct cattattccg gcaggcggta atatcatgcc
      541 ccatggcgtt tttcaaaaaa gcttcgcctt cttcccgacg gaccttccag atatccgaca
      601 cggccacaaa atcaaaattc agctccttgt aatgattcag gaaactggga aacaatgcct
      661 gacggaaccg gtccgaaaaa ccaatagccc cgagccttac ccggtcattg gcccccagga
      721 tacgaccata gcttttggca gacatactca tggcgccgag gtacaggcct gcggcagtga
      781 gggaggagtt cttgataaac tgacgacgtg atttcatttg aaaagtttag atgtttagtc
      841 gtttagccgt ttaggagctt agttagttta gcattgaaaa aagataatct ccctctaaac
      901 aactaaacac ctaaacaact aaaccctatt taagtttctt tatcttaacc gagcgatacc
      961 acacttcatt cccatgatcc tgcaaaagga tatgcccttt ctccgcttcc ccgaagtttt
     1021 tccaaaccac atatttactg atggcgacca ggtcgcggaa ttcttttgat ccacggatat
     1081 attccagcac ttttaccccg ttgagataat gttccacttt gttattggga tagaccacca
     1141 cccgtcccag attccaggca ccgatgggac gcaggaaacg cttttgctta tccgctttga
     1201 tcaggtcata cagggaagcc agggtgcggt tcccgtcccg ccccagtttg gcatccggat
     1261 gtaaagtatc atcgagtacc tggtactctg gtccgatagc cgatccgctg gttttctcgt
     1321 tcagggtgac aaaatatttt accccactgt tcgcgccggg ggtgagttta aattcaaatg
     1381 aaagatcgaa agccctatac tgttcttgtg tgataatatc tccaccattg gcagcctctt
     1441 taccttctga agaaagtaca tgcatggttc ccccactgat atcccatccg gtggacggaa
     1501 aggccgttcc cttcgcactg atccagcctt tgctgctttt cccgtcaaac aacaaggacc
     1561 aatcatcctt ttgttcataa gcggtcagcg tattgggttt cagattcacc acatagactc
     1621 cgggaggaaa agcgctgggc gtcaatccgg ttgttttgat ccgcagattt ttaaacacca
     1681 ctttcttacc cgcatgggta gggtttccga tcgcatgtac ctgtagtccg ataaaaccct
     1741 tgctgtccag ggtatccact acataagcgg tggctattcc gttcacccag gtcttcattt
     1801 cattgccgat acactctatt ctataatgat taaagctatt gggtttatac gcgtattgag
     1861 cccgggcatt cagttgcatg gggtaaagcc attcccggcg accttcatca tacacaccgc
     1921 cggtccatct tctccaggtg ggatccactt cacattgccg gccataaact ttcccttcgc
     1981 cattattacc agccggatcg aaatgactgc gggtctggat cccggaattt ccttcttcat
     2041 cttcgaccca tacatccagc tccaggataa aatcaccata ttccttttcg gtacataaaa
     2101 aagtatttcg ggtattggct accgttgttc cgacaatcgc gccatcttcc actttataag
     2161 tggctttccc gccaagaatt ttccaaccag tgaggtcttt cccgtttccc aacggagccc
     2221 agccattctg actatttaca gtaataaagg aaaccagcaa gaggggtaaa cagaaatacc
     2281 ggaaacgttt catatgtatc gcttaagaag gtaagagaat gaaagtaaat gtaaggaatt
     2341 cagcaacgaa tcagcctgac ctgtatcgct gtttattcac ggttgttgca caattcaaac
     2401 accgcctttt cagcttactc atttttttat atcttaatgt tttaagactg atccggctga
     2461 ataattcaaa ataaagtatc atatgttcac ctgcacccga accgactcca ccaataccga
     2521 tttcaaaatg ctggttaatc agctggaccg gtatctcatg ggcatcaacg gagacgatca
     2581 tgattttttt gatcagttta ataaaattga taacatcaga catgttgtgg tgctctacct
     2641 cgatggccag cccgccggct gcggcgccat caaagaatat gaacccggcg ttatggaagt
     2701 caaacgcatg tttgtgcccc ctgcggcccg gggtaaagga ctggccaccg ctatcttaaa
     2761 agaactggaa gtctgggctg ctgaactcgg ctacgaaaaa tgcattcttg aaaccggtga
     2821 tatcatgccg gatgctattg ctttgtacaa aaagaatggg tatcgttcaa tccccaatta
     2881 cggtcagtat gtggatgtag cttcaagtgt ctgttttgag aagcatttgg caggttgaca
     2941 agttaatagg ttgacaggtt aacaagaacc cttcta
//
LOCUS       DAOPMW010000414         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_40706_length_2968_cov_7.094748, whole genome shotgun sequence.
ACCESSION   DAOPMW010000414 DAOPMW010000000
VERSION     DAOPMW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40706_length_2968_cov_7.094748"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLU11_14420"
     CDS             complement(<1..376)
                     /locus_tag="PLU11_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HPN60378.1"
                     /translation="MKRNSLLIILLLFCYTGTMAQTSNPKQYKYVFTVAKDGTGDYYT
                     IQDAINAMRVYPLAPITLYIRNGTYHEKIEVPATNTDISFIGENVDSTIIVFDDYSGK
                     GKHTTFTSYTAKISGNRFRAANI"
     gene            complement(400..1152)
                     /locus_tag="PLU11_14425"
     CDS             complement(400..1152)
                     /locus_tag="PLU11_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HPN60379.1"
                     /translation="MKKTVILLFSLSLLSFMLPEKKKLKIFLAGDSTISIKEPRYYPE
                     TGWGMPFVYFWDSTVTVVNKAKNGRSTSSFRNEGLWKQIMDECREGDYVFIQFGHNDE
                     VPTKKTYTTETEFKANLVRYVTETREKKVTPLLLTPVARRKFNAAGIIEGTHDVYAQI
                     VRDVAKENGVLLIDLDKKSQELYQQMGVENSKLLFVQLKPGEHPHYPEGKEDNTHFSE
                     LGARLIAQIVLKELKTLHTDLNDRIYLPPVKK"
     gene            complement(1149..2573)
                     /locus_tag="PLU11_14430"
     CDS             complement(1149..2573)
                     /locus_tag="PLU11_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPN60380.1"
                     /translation="MKKQWSILPLLLFICASSCAQPVQQAVSTDPSVAIAFPGAEGFG
                     KFTSGGRGGQVMIVNTLADDGEGSLRKAVNTKGAKIIVFAVSGTIHLTSPLSIKTNTT
                     IAGQTAPGDGICLADYPVSLGGDNIIIRYMRFRMGDRFQSQKGMVDGSGGDDAFGGMK
                     RKNIIIDHCSVSWSTDEVFSVYAGDSTTLQWNIISEPLNYSYHFETGDKDWEHHGFGG
                     IWGGLHLSAHHNLIAHCTNRCPRFNGIRHTPTELVDYRNNVIYNWGSNNIYAGEGGSY
                     NIVNNYFKYGPSTNKNVRYRICNPGKRDPDIGFGSWHVSGNYVDESRPVTNDNWLGVD
                     MGNSGTEEDKKKFVIKEAHPAEALPVEAATVSYEAVLLSAGASLRRDTLDQRIVNDVR
                     NRTGRFIDVQGGFPHGTAYELTVNAWPALRSAPAPADTDKDGMPDAWEKEKGLNPADP
                     ADATKTGLLSYYTNIEVYINSLVK"
     gene            complement(2664..>2968)
                     /locus_tag="PLU11_14435"
     CDS             complement(2664..>2968)
                     /locus_tag="PLU11_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60381.1"
                     /translation="YMAGYPSYSLTNTLPASMYLITNTTADNQQVGGIWFNSLYKTAS
                     TSTPSGTTKIVWVGTTVNTVCAARYATGYVAGKGELLPLPQPAIDANFNLRQNPGY"
BASE COUNT          702 a          675 c          718 g          873 t
ORIGIN      
        1 tgatattggc cgcccggaac cggttacctg aaattttcgc agtatacgaa gtgaaggtgg
       61 tatgttttcc ttttcccgag taatcatcaa atacaatgat ggtgctgtcc acgttttccc
      121 cgataaaact gatgtcggta ttggttgccg ggacctcaat tttttcgtga taggtgccgt
      181 tacggatata cagggtaatc ggggctagcg gatatacccg catggcatta atggcatcct
      241 gaatagtata ataatcacca gtcccgtctt ttgcaaccgt aaacacatat ttatattgtt
      301 tcggattgct ggtctgggcc atagttccgg tatagcagaa caacagtaag attatcagga
      361 gactgtttct tttcatggtt tgtctgattc gggattccac tattttttta ccgggggtaa
      421 ataaatccgg tcattcaggt ctgtatgaag tgttttcagt tcttttaata cgatttgcgc
      481 aatcagccgg gcacccaatt cactgaaatg ggtgttatct tccttccctt ccggataatg
      541 cggatgttca cccggtttga gttgaacaaa gagcagtttt gaattttcca ctcccatttg
      601 ctggtagagt tcctgacttt ttttatcaag gtcaatcagc aacaccccgt tttctttggc
      661 cacatccctg acgatctgcg catatacatc atgggtgcct tcaataattc ctgctgcatt
      721 gaattttcta cgggccacag gtgtgagcag gagcggggtg acttttttct cccgggtctc
      781 ggttacatac cggaccaggt tggccttgaa ttctgtttct gtggtatagg ttttcttagt
      841 aggtacttca tcattatgtc caaactgtat gaaaacataa tctccttccc ggcattcatc
      901 catgatctgt ttccagagcc cttcatttct gaacgaactg gtactgcggc cgtttttggc
      961 tttgttaact actgtaacag tactgtccca gaaatacaca aagggcattc cccatccggt
     1021 ttcgggatag taacggggct ccttgatgct gatggtcgag tcgccggcca ggaaaatttt
     1081 tagtttcttt ttttcaggca gcataaaaga taaaagcgat aggctgaata ggagaatgac
     1141 tgtttttttc attttaccag gctgttaatg tacacttcaa tattggtata atagctgagt
     1201 aatccggttt ttgtggcatc cgccggatcg gccgggttca gtcctttttc cttttcccag
     1261 gcatcgggca tgccgtcttt atcggtatct gccggtgcag gggctgaacg aagtgccggc
     1321 caggcattaa cggtcaattc atacgcggtg ccatgcggga aacctccctg tacatcaatg
     1381 aaccgaccgg tgcggttacg tacgtcattc acgatacgtt ggtcgagggt gtctctgcgt
     1441 aaactggctc ccgctgatag caggactgct tcataggaaa cggtggccgc ttctacgggt
     1501 agtgcttcag ccggatgggc ttctttaatg acaaatttct ttttatcttc ttcggttccg
     1561 ctattgccca tatccacgcc caaccagttg tcgttggtca caggacggga ttcatccaca
     1621 tagtttccgg acacatgcca gctgccaaag ccgatatccg gatcccgttt tcccgggttg
     1681 cagatgcggt acctgacatt tttattggtg ctgggtccgt atttgaaata attgtttact
     1741 atattatatg acccgccctc accggcgtag atattattac tgccccagtt atagatcaca
     1801 ttattccggt aatcaactaa ttcggtcggg gtatggcgga tcccattgaa gcgtggacaa
     1861 cggtttgtgc aatgcgcaat gaggttgtga tgggcactta aatgaagtcc gccccagatg
     1921 ccgccaaagc catgatgttc ccagtcttta tcacctgttt caaaatgata ggaatagttc
     1981 aggggctcac tgatgatatt ccattgcagg gtggtgctgt ctccggcata caccgagaac
     2041 acttcatccg tactccagct gacactgcaa tgatcgatga tgatgttttt ccgtttcatg
     2101 ccgccaaatg catcatcacc accgctgccg tccaccatac ctttctgtga ttgaaagcgg
     2161 tctcccatcc tgaaacgcat ataccggatg ataatattat ctcctccaag tgaaaccgga
     2221 taatcggcca ggcagatgcc atcgccaggg gcggtttggc cggcgatcgt ggtatttgtt
     2281 tttatgctta acggactagt aagatgaatg gtaccggaca ctgcaaagac aataatctta
     2341 gctccttttg tattcacggc ttttcggagg cttccttctc catcatcggc cagggtatta
     2401 acgatcatca cctggcctcc tcgtccacct gaggtgaatt tgccaaaccc ttctgctccg
     2461 gggaaagcaa tggcaacgga agggtcagtg gaaactgctt gttgtaccgg ctgggcacag
     2521 gagctggcac aaatgaaaag caggaggggc aggattgacc attgtttttt cataatgaaa
     2581 agaaggggtt gaagtaaata aggctgcatc ggtaaaaaat tactgatgca gcctcattag
     2641 tggttcgtta tacaaagaac ttgttaatat cccggattct ggcgcaaatt aaagttggcg
     2701 tcaatagctg gttgcggtag gggtaatagt tctccttttc cggcaacata accagtggca
     2761 tagcgggcgg cgcaaacagt attgaccgtg gtacctaccc atacaatttt ggtggtgcct
     2821 gaaggagtag aggtagatgc ggttttgtac agagaattaa accatattcc cccgacttgc
     2881 tgattatcag ctgttgtgtt tgtaatgagg tacatggagg cgggcagtgt attggtcaga
     2941 ctataggaag ggtaacctgc catataag
//
LOCUS       DAOPMW010000415         2955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_40969_length_2955_cov_8.330000, whole genome shotgun sequence.
ACCESSION   DAOPMW010000415 DAOPMW010000000
VERSION     DAOPMW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40969_length_2955_cov_8.330000"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2389)
                     /locus_tag="PLU11_14440"
     CDS             complement(<1..2389)
                     /locus_tag="PLU11_14440"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPN60382.1"
                     /translation="MKNILLFSKTKMKLVDLLPGIATVWHSRFYPEKQIRGASKPGWL
                     RWLAVLLLLACFSPDARSTSLTTVVKGISITVSADDGVLPAGTTLDVRLLTNVEKRGY
                     LELLRSDRGITLRQSMAFDITLHDQQGNEIQPGGEVDVSFSGLNLDGQPEELVVFHAV
                     GSGRNAEKGFAFDKKYPGIQSSGGLKSGTIPLKPERGKLKFSTSHFSIYLVGTTNTAT
                     YNFYVGASLVDTQIVLNGESLFEPEAPEAIEGKRFTGWYEGTTQVVFNTPVTVESTET
                     YTVNANFEDIWYVYFKYSGNIIATKEVVPGGTVDATGVPLIVTETGKAFLHWSASSGG
                     SAFDFESPINANTTLYAVLADMWTVSFNTQGGSTTLPKYIADGQSLGTVEDPIRPGFT
                     FGGWYTEPDGAGTEYTSSTTIEESVILYAKWTGLPVDYTIIYWQENADDANYTYKETA
                     TGSGTAGTILTLPPQAISNTRYQYFSFDYYDEGLTLNGDGTTTVNVYYSRNSYTVIFD
                     LDRTGATLNIGGETYTGSEYTFTAKYDSDISALWPTATNIPNVGLYKFTGWTYPGGSL
                     TLVSKRMNFTPDLFSTTGTRTYEGNWAINVVTYELHYMIQVTSGTGEREYNGTWYNED
                     MAYNQMANTTGGGWSAKQISGVTNVGIQQDRTAGPDGDIDVYFYYNRNSYNLTFYNYN
                     ENDKELNFVFGASIEDTEYTPLKPSVLPEGYTFGGWYDNEATSGDPFDFEGAVMPPNN
                     LILYSKWITPVYTVRIHEVMEDPGPGGNSYTLELPTGSTIDESSLIKEEVPGVAFI"
     gene            complement(2612..>2955)
                     /locus_tag="PLU11_14445"
     CDS             complement(2612..>2955)
                     /locus_tag="PLU11_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60383.1"
                     /translation="HLLVMSHVPVQVVAGTLTYDFTAQQSYILPGSGLWGQKEVDGIF
                     FMFGGNGNQSANADSDTDINADDQSTWESHNGRQGYRSGDYNLNGDTNSNDRIIWERN
                     NGHGTSVIRNY"
BASE COUNT          738 a          705 c          689 g          823 t
ORIGIN      
        1 cgataaaggc caccccgggt acttcttcct tgatcaggct gctttcatca atggtagacc
       61 cggttggaag ctccagagta taggaattgc cgcccggtcc cggatcttcc atgacttcat
      121 gaatacggac cgtgtacacc ggggttatcc attttgaata gagaataaga ttgttgggag
      181 gcatcaccgc tccttcaaaa tcaaacggat caccgctggt cgcttcatta tcgtaccatc
      241 cgccaaaagt gtagccttca ggtaaaacgg atggctttag cggggtgtat tcagtatcct
      301 cgattgatgc tccgaaaacg aaattaagtt ctttatcgtt ttcattataa ttgtaaaatg
      361 tgagattgta ggaattccgg ttgtaataga aataaacatc gatatctcca tcgggccccg
      421 ctgtgcgatc ctgctgaata cctacgtttg taacaccgga aatctgttta gcactccaac
      481 cacctcccgt ggtatttgcc atctgattgt aggccatgtc ttcattgtac caggtgccgt
      541 tatattctct ttctccagtt cccgaagtca cctggatcat gtaatgcaac tcataggtga
      601 caacattgat agcccagttt ccttcatacg ttctggtgcc tgttgtggaa aacaggtcag
      661 gtgtaaagtt catccgtttg gaaactaacg ttaaactacc accaggataa gtccaccccg
      721 taaacttgta aagtccaaca tttggaatgt ttgttgcggt tggccataat gctgatatgt
      781 cggaatcata tttggcggtg aaggtatatt cgctgccggt ataggtttct cctccgatgt
      841 tcagggtggc ccctgtcctg tccaggtcga atattaccgt ataactattc cttgagtaat
      901 atacattgac agtagtagtt ccgtctccgt tgagggttaa accctcatcg taataatcaa
      961 acgagaaata ctgataacgg gtgttgctga tagcctgggg cggaagcgtg aggattgttc
     1021 cggcagtacc cgatcctgta gctgtttcct tgtacgtgta attggcatcg tctgcatttt
     1081 cctgccagta tataattgta tagtcaaccg gcaggcctgt ccatttggca taaagaatca
     1141 cactttcctc aatggtggta gatgaggtat attctgttcc cgctccatcg ggttcggtgt
     1201 accatccacc aaaagtaaat cccggcctga tgggatcttc cacggttccc agggattgcc
     1261 cgtcagcaat gtacttcgga agggtggtac tgccgccctg agtattaaag gagaccgtcc
     1321 acatatcagc aagaacggca taaagggtgg tattggcatt tatcggactt tcaaaatcaa
     1381 aagcagaacc gccagatgaa gcggaccagt gtaagaaggc ttttcctgtt tccgtcacaa
     1441 tcaaaggaac gccggttgca tcaacggtac ctcccggaac cacctccttg gtggctatga
     1501 tatttccgct gtatttaaaa tacacatacc agatgtcctc aaagttcgca ttaacggtat
     1561 aggtctcagt tgactccaca gttacgggtg tattgaaaac cacctgtgtg gttccttcat
     1621 accatccggt aaacctttta ccttctattg cctccggtgc ttcgggttca aagagggatt
     1681 caccgttcag aacaatctgg gtatcaacca gggaagcacc aacataaaaa ttataggtgg
     1741 cagtattggt agttccgacg aggtagatcg agaagtgcga agtagagaac ttcaactttc
     1801 ccctttcggg ttttaagggg attgttcctg atttcagtcc acctgatgac tgtatccccg
     1861 ggtatttttt atcgaaggca aatccttttt cagcgttcct gccactacca acggcatgga
     1921 aaaccaccaa ttcttcaggc tgtccatcca ggttaaggcc cgagaagctg acatccacct
     1981 ctccgccggg ttgaatttca tttccctgtt ggtcatgaag ggtaatatca aaagccatgg
     2041 attgccggag ggttattcca cggtctgaac gcagtaattc gaggtaaccg cgtttttcaa
     2101 catttgttaa aagccggacg tcaagtgttg ttcctgccgg aagtacgccg tcgtcagccg
     2161 aaacggtaat gcttatcccc ttcaccaccg tagtcagtga agttgaccgg gcatccggcg
     2221 aaaaacacgc caggagcagc aggactgcaa gccagcgtag ccatcccggt tttgaggcgc
     2281 cccggatttg tttctccggg taaaacctgg aatgccagac cgttgcaatt cccggcaaca
     2341 ggtcaacaag cttcattttt gttttcgaaa aaagtaaaat gtttttcata gttactaaaa
     2401 ttgagtttga agcaatttat gaagaatata gagttaagaa cgattaaaga atatgtttta
     2461 taacatattt ttgcttttta acaataaatt aaaaaattca aaagtgctta aaatctgccc
     2521 ggacagcgac ttaggaggca ttggaccggg caattagcat aaaaagccga atgtaatttc
     2581 ttaacattcg gcttttggtt ttaacagatc attagtagtt cctgatcacg ctggtaccgt
     2641 ggccgttgtt ccgctcccag atgatccggt cgttgctgtt ggtatccccg ttcaggttat
     2701 agtcgcccga ccggtacccc tgcctgccgt tatggctttc ccatgtgctc tggtcgtcgg
     2761 cgttgatgtc ggtgtcggaa tcagcgtttg ccgactggtt gccgtttccg ccgaacataa
     2821 agaagatccc gtcaacttct ttctggcccc acagacccga tcccggaagg atgtagctct
     2881 gctgtgccgt gaagtcgtag gtcagcgttc cggccacgac ctgtaccggc acgtgcgaca
     2941 tcaccagcag gtggt
//
LOCUS       DAOPMW010000416         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_41073_length_2951_cov_3.090815, whole genome shotgun sequence.
ACCESSION   DAOPMW010000416 DAOPMW010000000
VERSION     DAOPMW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41073_length_2951_cov_3.090815"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2951)
                     /locus_tag="PLU11_14450"
     CDS             complement(<1..>2951)
                     /locus_tag="PLU11_14450"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN60384.1"
                     /translation="TRLAIPALDLLMLTHGYRYFDFIDYVVKEGALKHLPDQSNILSG
                     VVQDLSGNPVQARLFILQNTGGGMGMELTTGNDGTFFFSDLLSNSQYVLFAQSLNKKG
                     RVVIHILQNGIGYNPATARSFSLLAGKPLNFEGIPASPVLNAIRQKQNPALFKRTPDE
                     EIIGGTEVVVTSAYGMKRRRDITGSVAYVRADELLDANRLGNVLQGRVAGLHVMNKSN
                     YLDGSQIRIRGMAAIYGENEPLYIINGVPVSKLFLNTMNQNDIESITVLKDGNATALY
                     GSAAAYGAIIIESKTYRRQRIQLKFGNRYYYSSQLVNTSGANYTPVRKFYAPKYERTE
                     TDERTDFRETIYWNPVVQTNKEGIASVEFYNSDASSTFRAVAEGLGYNGMIGRTEHTY
                     VVQQALQLDAKIPPYLTVGDKALLPLVIRNNSTEAGTYQLNITPPEKFILGPYSNTVS
                     LQPDSSIQLLIPLETGTATSGIIRFSVTGPSSKEVLSLPVAATDNGFPVKMVFSGNQD
                     KQHQFTIANMVPGTLRTNLRLFKSLEGQLVNGIESMLREPHGCFEQTSSSTYPNIYIL
                     KYLKEHGKSNPEIEKTALEYIRQGYKKLIGFETSQNGFEWFGNTPPHEALTAYGLLEF
                     TDMKEFVEVDEKMLERTKKFLMSRRDKEGGFRIQNKGYDQFASVPNKIAHLYIVYALT
                     QAGIGKEIEPEYKSAVKKAFESKDAYQLALMALAASNMKDQANYLALMQTLNQLPEKL
                     NAETSVVNSRDASLRVESWSLHALALLRSANPDLAKAAGLLSNILAEKNYYGYGSTQS
                     TVLALKALTEYARVSGKVSENATVHFKVNDTVLTDYAGVNPLLQTDTNVFAVTYEKKE
                     GAVPYNLELSYNTYLPPTNPDAELKLKTTLKSSETTVGETVRMDIEIINTKSVLQPMA
                     IAKIGIPAGLSIQPWQLKEFMEKNKCAYYEIFDNYLVFYWMGFAPNETKTLGLDLKAE
                     IPGTYKAKAST"
BASE COUNT          687 a          676 c          669 g          919 t
ORIGIN      
        1 ccgtgctggc ctttgcttta tacgttccgg ggatttcggc tttcaggtcc agtccgagtg
       61 ttttcgtttc gttgggggca aaacccatcc agtaaaacac gaggtagtta tcaaaaattt
      121 cataataggc gcatttgttc ttttccatga actctttcag ctgccagggt tgaatggaca
      181 agccggccgg tataccgatc ttggcaatgg ccatgggttg cagtacggac tttgtattga
      241 ttatctcaat gtccattctt accgtttcgc ccactgttgt ttcactgctc ttcagcgtag
      301 ttttcagttt cagctcagca tcgggattcg taggtggtaa gtacgtgtta tatgagagtt
      361 ccagattata cggaacagct ccttcttttt tttcataggt tacagcaaat acattagtat
      421 cagtttgtaa gagcggattc actcctgcat agtcagtcaa gacagtatca ttgactttaa
      481 aatgtacggt tgcattttct gacacttttc cactaacccg ggcatattct gtcagggctt
      541 tcagcgccag caccgttgac tgggtggagc cgtatccgta atagtttttt tctgcgagga
      601 tattagagag aagccctgct gcttttgcaa gatcagggtt agcagatctt aataaagcca
      661 gtgcatgcag gctccatgat tccactctta aggaggcatc tcttgaattg acgacagatg
      721 tttctgcatt tagtttttca ggtaattgat tcagggtctg catcagggcc aggtagtttg
      781 cctggtcttt catgttgctg gcggccagtg ccatcagggc cagctgatag gcatccttgc
      841 tttcaaaggc ttttttcaca gcggatttgt attcaggttc gatttctttg ccaataccgg
      901 cctgcgtcag tgcatataca atatatagat gcgcgatttt atttggtact gaggcaaact
      961 ggtcatagcc cttattttgt atccggaatc caccttcttt gtcccttcgg ctcatcagaa
     1021 attttttcgt gcgttccagc attttctcat ccacctctac gaattctttc atatcggtga
     1081 attcaagcaa cccgtaagcg gttaatgctt catgtggggg tgtgttccca aaccattcaa
     1141 acccgttttg gctggtttca aacccgatca gttttttata tccctgccgg atatactcca
     1201 gtgcagtctt ttcgatttcc ggatttgact ttccgtgttc tttcaggtat ttcaggatat
     1261 agatattcgg ataagtgctg gaagaggttt gttcaaaaca tccatgaggt tcacgcagca
     1321 tggattcaat tccatttacc agctggcctt ccagtgattt gaataaccgc aggttggtac
     1381 gcagggtgcc cggcaccata ttggcaatgg tgaactggtg ctgtttatcc tggttacctg
     1441 agaataccat tttcaccggg aacccgttat cggtagcggc gacaggcaat gaaagtactt
     1501 cttttgagga agggcccgta acactgaaac ggatgatgcc agatgtagcg gtgccggttt
     1561 ccaggggaat cagtagttgt attgaactat cgggttgtaa gcttaccgta tttgaataag
     1621 gacccaggat aaatttctcc gggggagtga tatttaattg gtaggtaccg gcttcagtac
     1681 tgttattcct aataaccagc ggcaacaggg ctttatctcc cacggtgagg tatgggggta
     1741 ttttggcatc cagttgcaat gcttgttgta caacataagt gtgttctgtc cggccaatca
     1801 tcccattata ccccagtcct tctgcaacgg cacgaaaagt gctggaggca tccgaattgt
     1861 aaaactcaac actggctatc ccctctttat ttgtttgtac cactgggttc cagtaaatgg
     1921 tttccctgaa atcagtccgt tcatcggttt ccgttctttc atacttcggc gcatagaatt
     1981 tccgtactgg tgtgtaattg gcaccgctgg tattcaccaa ttgggaagaa taataatacc
     2041 ggttcccgaa ttttagctgt atccgttgcc gccggtaagt ttttgattct ataatgatcg
     2101 ccccgtatgc ggcagctgat ccatagagcg ccgtggcatt gccatcttta aggaccgtga
     2161 tggactcaat atcattttga ttcatagtat tgagaaatag ctttgatact ggtacgccat
     2221 taattatata aagaggttca ttttcgccat aaatggcagc catacccctt atcctgatct
     2281 gtgatccatc caggtaattt gatttattca ttacatgtaa gcccgccact cttccctgga
     2341 gtacattgcc tagccggttc gcatccagca attcatccgc cctgacatat gccactgaac
     2401 cggtaatgtc tctccttctt ttcataccat aggcacttgt tacaaccact tctgttccgc
     2461 ctattatttc ttcatctggg gtccttttaa aaagtgccgg attttgcttt tgccggatcg
     2521 catttagtac cggggaagct ggtattcctt caaagttgag cggtttaccg gcaagcaggg
     2581 aaaaagacct ggcggttgcc gggttatacc cgattccatt ttgcaggata tgtatgacca
     2641 ctcttccttt tttattgagt gattgggcga aaagaacata ctggctgttg gatagtaagt
     2701 cagagaaaaa gaaagtacca tcgttacccg ttgtcaactc catccccatg cccccacctg
     2761 tattttgcag gataaacagc cgggcctgta ccgggttgcc actgaggtcc tgaaccacgc
     2821 cgcttaaaat attactctga tcgggcaggt gtttcaatgc accttctttt accacatagt
     2881 caataaaatc aaaatagcgg taaccatggg tcagcatcag cagatcaagt gccgggatgg
     2941 ctagtctcgt c
//
LOCUS       DAOPMW010000417         2922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_41628_length_2922_cov_8.198116, whole genome shotgun sequence.
ACCESSION   DAOPMW010000417 DAOPMW010000000
VERSION     DAOPMW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41628_length_2922_cov_8.198116"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLU11_14455"
     CDS             complement(<1..332)
                     /locus_tag="PLU11_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HPN60385.1"
                     /translation="MNLTAQQSNEFTGRHIGPRDADTRSMLKTIGEDSLAALINKTVP
                     PGIRMAGALNIPAAMSEHEYLKHIQEVSLKNKLFKNYIGQGYYDTITPSVILRNIFEN
                     PGWYTQYTP"
     gene            535..1026
                     /locus_tag="PLU11_14460"
     CDS             535..1026
                     /locus_tag="PLU11_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPN60386.1"
                     /translation="MTGDLLKNWEKKSKERQKIYKNYLQRVSKNTVLRQLPDYHEEAF
                     SKVDCLQCANCCKNFSPRFKTPDIKRLSKLLRLRESDFIETYLKVDEEGDYVANTSPC
                     PFLGADNYCSIYEDRPSDCRRFPYTDEDVIFKRQPLTLKNSTFCPITYYVLEKLVQAE
                     KQG"
     gene            complement(1158..1658)
                     /locus_tag="PLU11_14465"
     CDS             complement(1158..1658)
                     /locus_tag="PLU11_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPN60387.1"
                     /translation="MMMMNRKRRGMVVALLILLSAGLFFYISYNNQQVRSEKDLYLLT
                     DRFRDYNWSDNGKGSSLTFKLYSYKEKFVVDPAFLAGLKGDEFKSTNMGDSITIGIPA
                     YALPYLNTRQKRIPVYSIQIKGKPLLDASYTLKKKNSNALMIAGSLVLVAGLVVYFTK
                     RRGDSR"
     gene            1925..2818
                     /locus_tag="PLU11_14470"
     CDS             1925..2818
                     /locus_tag="PLU11_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN60388.1"
                     /translation="MNRRNFLTQTALSAGALTLLQNDILTALFQEPWKISMLTSDIGV
                     FTERGGTILFYLSKKGIVVVDSQFPDQSKHLIDELKKKSEKPFKVLINTHHHGDHSGG
                     NISFKGLVEHVVAHENSKINQEKTAKQNKNEDKQLYPDQTYTSTWCQKLGKEKICLHY
                     FGAGHTNGDSFVHFQHANIVHCGDLVFNRRHPYVDRSAGANMKSWIIVLDKALQTFDA
                     KTRFVYGHAGTGFDITGNTDDLKAFRDYIGNVLKFVEGEMKSGKSKEEILKATIIPGS
                     EAWKTEGIQRPLQAAWEELKG"
BASE COUNT          817 a          627 c          714 g          764 t
ORIGIN      
        1 ggggtatact gggtatacca tcccggattt tcaaaaatat tccggaggat caccgagggg
       61 gtgatcgtat cataataccc ttgcccgata taattcttga ataacttatt ctttaaggat
      121 acttcctgaa tatgcttcag gtactcatgt tcactcatgg ccgccgggat attcagggca
      181 cccgccatcc ggatgccggg aggtacggtt ttattgatca gggcggccag gctgtcttcg
      241 ccgatggttt tgagcatgga acgggtgtcc gcatcacggg ggccgatatg ccttccggta
      301 aactcattcg attgttgtgc tgtcagattc atattgagga atattcaggt atttatcagg
      361 aaagtccggg cggtcaggtg gggcaaacgg tcaggaatac gggtcgtaaa actgaaattg
      421 ttagccggga gtgacggtac gacttttttg cccggcaaag ttacggatga gggggcagaa
      481 aaatgctgtt ttgtggaagc tggggataag ccgtgaaata ccgttctttg tggtatgaca
      541 ggtgaccttt taaaaaactg ggaaaagaaa tcgaaggaac ggcagaaaat ctataagaac
      601 tacctccaac gggtcagcaa gaacacggtt ttgcgccaat tgcccgatta tcatgaggag
      661 gctttcagca aagtagactg cctgcaatgc gccaactgct gtaagaattt ctccccccgg
      721 ttcaaaaccc ccgacattaa aaggctgagc aaactgctac gtctgcggga aagcgatttt
      781 atcgaaacct acctcaaagt agacgaggaa ggggattatg tggccaacac cagtccttgt
      841 cctttcctgg gtgccgacaa ctactgttcc atctacgaag accgtccttc cgattgccgg
      901 cggtttccct atactgatga ggatgtgatc ttcaaacgac aaccgctgac acttaagaat
      961 tctacctttt gtccgatcac ctattatgtg ctggagaagt tggtgcaggc ggagaagcag
     1021 ggataggttg gaggttgggg gttcgaggtt cgaggttgga ggttggaagt tggaggttcg
     1081 aagagactga aagtagggag gggatgaaag gttgccaggt tagtaggggc tgtttactaa
     1141 tgactaacga ctaatgacta acgactatct cccctgcgtt ttgtaaaata aaccaccagc
     1201 ccggccacca gcaccagtga tcccgcaatc atcagggcat tactgttttt ctttttcagg
     1261 gtgtaggagg catccagcag cggcttgcct ttaatctgaa tcgaatagac gggaatcctc
     1321 ttttgccggg tattgagata gggcagggca tacgcaggga taccgatggt aatcgaatct
     1381 cccatattgg tggatttaaa ttcatctcct tttaaccccg ctaaaaatgc cggatcaact
     1441 acaaattttt ctttataact ataaagcttg aacgtaagtg atgaaccttt accattatcc
     1501 gaccagttgt aatccctgaa ccggtccgtc aggaggtaca gatctttctc agaacggacc
     1561 tgttggttat tgtaactgat atagaagaac agacctgcag acagcagtat caacaaggca
     1621 acgaccatgc ctcttctttt tcggttcatc atcatcatgt agttaaattt tatatgatac
     1681 tcgcttgcga agctacaaac attagcccga catcagcctg gttctgttga atagcttttt
     1741 cctcctgatt cggtctttgt ctatgaatat tcagtaaatt gaacatgcat tattttaccg
     1801 acacaacctt tttacctctt taaccgtttt caatcatgaa tataactccg aacttttaac
     1861 ctcgaacctc caacctccaa cctcgaacct ccaacctcca acctcgaacc tccaacttta
     1921 taatatgaat cgccggaact ttcttacaca aaccgccctc agtgcaggag ccctgaccct
     1981 cctgcaaaat gatatactga ccgctttatt ccaggaaccc tggaaaataa gtatgctgac
     2041 cagtgatatc ggggtgttta cagagagggg ggggacgatt cttttttatt tatcaaagaa
     2101 aggaatcgtc gtagtggatt cgcaattccc cgaccagagt aaacacctga ttgatgaatt
     2161 gaagaaaaaa tcagaaaagc catttaaggt cctgatcaat acccatcatc acggggatca
     2221 ttcaggaggt aatatttcat ttaaagggct ggtggaacat gtggtggccc atgagaactc
     2281 aaagatcaat caggaaaaaa cagcgaaaca gaataagaat gaggataaac aattgtatcc
     2341 tgatcagact tataccagca cctggtgcca gaaactgggc aaggaaaaga tatgtctcca
     2401 ttatttcggt gccggtcata ccaatggcga tagttttgtt catttccagc atgccaacat
     2461 tgtgcattgt ggcgacctgg tgttcaaccg ccggcatcca tatgttgacc gtagtgcggg
     2521 tgccaatatg aaaagctgga tcattgtatt ggataaagca ctgcaaacct tcgacgccaa
     2581 aacccgtttt gtatacggac atgccggcac cgggttcgat attaccggta acaccgatga
     2641 cctgaaggct ttccgtgatt atattggaaa cgtactgaag tttgttgaag gtgaaatgaa
     2701 atcaggaaaa tcaaaagaag agatcctgaa agccactata attcccggta gtgaggcctg
     2761 gaaaacggaa gggattcaac ggcctttgca agcagcctgg gaagagttga aggggtaggg
     2821 atgttggagg ttggaggttc gaggttggag gttggaagtt cgaggagttt aaaaggttga
     2881 aaaggtttaa aaggtttccg aggttaactt aatcacgctc tc
//
LOCUS       DAOPMW010000418         2916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_41760_length_2916_cov_6.238728, whole genome shotgun sequence.
ACCESSION   DAOPMW010000418 DAOPMW010000000
VERSION     DAOPMW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41760_length_2916_cov_6.238728"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="PLU11_14475"
     CDS             <1..1622
                     /locus_tag="PLU11_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPN60389.1"
                     /translation="AQGEPFTKRTVVSGLSSAWEVLYGPNDSLWITENSAYLISRIDI
                     ATGAKTVLVDLRATDASINFLSSVANPPQGGLMGLVIHPNLYSSDPAVRAAKPWVYAA
                     YVYNRGSCPGTNTSCIYTTKIVRFTYSGNTLNSPVTIINTIPGSSDHNSGRLVLSPVI
                     EPGADAAHTQYRLYYTVGDMGAGQFKNTTRTQNAQNVNIMEGKVLRLNTESDGDAGQD
                     AWIPNDNPFFNASSITPQDYVFTQGHRNAQGLAWGNVNGTNILYSTEQMDRTDDEVNI
                     IEAGKNYGWDQVSGYCDDNVNGYKIGQNTSANEGAFCSVPANNHQEPIFTTFTEPASG
                     MAALFAQPNNQLWPTIASSSLDFYGSGKIGGWKHSLLISPLKKDRVYRVKLNAAGTDV
                     IGDTIPYFAGDGNRIRRIRVSPDALKFYVARDAGATSNGGAIMEYTYAGTVLSLNENP
                     GEGPVRVHDMISIYPNPVSDILNIHGKRDLRKPLFAQVMDVNGRLLLTGSSFRNDFTM
                     DVQTLQAGTYFLKLYNGNNMEVQVVKFVKVK"
     gene            complement(1897..2124)
                     /locus_tag="PLU11_14480"
     CDS             complement(1897..2124)
                     /locus_tag="PLU11_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60390.1"
                     /translation="MKSNARPLSSFIAPQLTAVSKGKTASSEQIKSAYLKAILSGSRI
                     ISGTEKAMPVENPDRAAAIRYIRESATKKNK"
     gene            complement(2265..>2916)
                     /locus_tag="PLU11_14485"
     CDS             complement(2265..>2916)
                     /locus_tag="PLU11_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPN60391.1"
                     /translation="HIATLTKLPFVTAENKFEALAAHDAIVEAHGALKTVAVSLMKIA
                     NDIRMLSSGPRSGIGELFIPDNEPGSSIMPGKVNPTQCEALTMIAAQVMGNDVAINIG
                     GANGHFELNVFKPVMIYNFLHSARLIGDGCVSFNDKCAVGIEPIEANIRKHVENSLML
                     VTSLNTKIGYYKAAEIAQKAHKQGTTLKEMAVQLGYVTPEQFDEWVIPANMVGKLK"
BASE COUNT          775 a          631 c          713 g          797 t
ORIGIN      
        1 ttgctcaggg cgaacccttt acaaaaagga cagtcgtttc cggtctcagt tcagcctggg
       61 aagtccttta tgggcccaac gattctctct ggataacgga gaacagcgct tacctgattt
      121 caagaattga tattgcgacc ggtgctaaaa ccgtattagt ggatctccgg gctacggatg
      181 ccagtatcaa ctttttatcc tcggtggcca atccgccgca gggaggatta atgggactgg
      241 tgattcatcc caacttatat tcatccgatc cggcggtaag ggctgccaaa ccatgggttt
      301 atgcagcata tgtctataac cgtggcagct gtccgggtac taatacaagc tgtatctaca
      361 ctaccaagat tgtccggttt acttattctg gaaatacact caatagtccg gttactatta
      421 ttaatactat tcccggcagc agtgatcata actcggggcg actggtgttg agcccggtga
      481 ttgaacctgg ggcggatgcg gcccataccc aataccggct ttattatacc gtgggagata
      541 tgggggccgg gcagtttaaa aataccacaa ggacccaaaa tgcgcagaat gtaaacatca
      601 tggaaggaaa ggtactgcgg ttgaatacgg agagtgacgg agatgccggt caggatgcgt
      661 ggataccgaa cgataatcct tttttcaatg cttcatctat taccccacag gattatgtat
      721 tcacgcaggg acaccggaat gcacagggac ttgcctgggg aaatgtaaac ggtaccaata
      781 ttctttatag tacagaacag atggaccgga cagatgatga agtcaatatt attgaagccg
      841 gaaaaaatta tggttgggat caggtctccg gctattgcga tgataatgta aatggctaca
      901 agatcgggca gaataccagt gccaacgaag gggctttttg ttctgttccg gccaataatc
      961 atcaggaacc catattcacc acatttacag aaccggcatc tggtatggcc gccctgtttg
     1021 cccagcccaa taaccagctt tggccaacca ttgcatcatc cagtcttgat ttttatgggt
     1081 ctggaaaaat cggtggctgg aaacactcgc ttttaatcag ccctcttaaa aaagaccggg
     1141 tatatcgtgt aaaactgaat gctgctggta cagatgtgat tggtgatacg atcccctact
     1201 tcgccgggga tggcaaccgc atccggagga tacgggtatc acccgatgct ttaaaatttt
     1261 atgtggcccg ggatgcaggg gcaacaagta atggcggtgc cattatggag tatacttatg
     1321 ccggaacagt tttgtcactc aacgaaaatc cgggtgaagg cccggtccgg gtacatgaca
     1381 tgatcagcat ttatccgaat ccggtatccg atatcctgaa tattcacggg aaaagggact
     1441 tacgtaaacc gctttttgcc caggttatgg atgtaaacgg acgactgtta ctgaccggca
     1501 gcagtttccg gaatgacttt acaatggatg ttcaaaccct ccaggcgggc acctattttc
     1561 tgaaattgta caatggaaac aatatggaag tgcaggtggt gaagttcgtg aaagtgaagt
     1621 aagctgtaga aagaagcagc gctttagggg ctttattgat tattaagatt attaattatt
     1681 acataaagca gaagtgtctt ttcagttgcg atttgtccga aaatcattct gtaatatttt
     1741 gagttttcaa agccgtttac gctaataatt aatcatataa taattagtaa tcaactgcta
     1801 taacacagtt ggtattaaaa aaataacccc cgctttcacg ggggcttttt tttaatgctc
     1861 agggttcgaa ataaatacta acgcgttgaa gtttttttat ttattctttt tagtggcgct
     1921 ctcccggata taacggatcg cagcggccct gtccgggttt tctaccggca tggctttttc
     1981 tgtaccggaa atgattctgg atccggaaag aatagctttc aggtaagctg atttgatttg
     2041 ttccgaagaa gcggttttgc ccttggacac agcggttagc tgtggggcaa taaaggatga
     2101 caaaggacgg gcattacttt tcataaggcg gaggggttat ttcagaataa tgaattttac
     2161 ggaagtaagt tacattgatt tacaggccgg aaacaaactt ttaatcctaa aaaaaagaga
     2221 aaacagggtg ttttctcttt ttactaaggg gaaatactag ttttttactt cagcttgcca
     2281 accatattgg ccggtattac ccactcatca aactgttcgg gtgttacata acccagttgc
     2341 accgccattt ccttgagggt ggtaccttgt ttatgggctt tctgcgcaat ctctgccgcc
     2401 ttatagtagc cgattttcgt attgagtgaa gtgaccagca tcagtgaatt ttccacgtgc
     2461 ttcctgatat tggcttcaat gggctcaata cccacggcgc atttatcatt aaaggatacg
     2521 caaccatcac cgatcaggcg ggcactgtgc aggaagttgt agatcatcac cggcttgaac
     2581 acattcagtt caaaatgtcc gttggcaccg ccgatattga tggccacatc attccccatc
     2641 acctgggccg caatcatggt gagggcctca cactgggtgg ggtttacctt gccaggcata
     2701 atggatgagc cgggttcgtt atcgggaata aagagctcgc cgattccgct gcgtggaccg
     2761 gaagacagca tccggatatc gttggcaatc ttcatcaggc ttacggccac cgttttcaat
     2821 gcaccatggg cttccacaat ggcatcatgt gcggccaggg cttcgaactt gttttcggcc
     2881 gttacaaagg gcagtttggt cagggtggca atatgc
//
LOCUS       DAOPMW010000419         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_41914_length_2908_cov_8.130389, whole genome shotgun sequence.
ACCESSION   DAOPMW010000419 DAOPMW010000000
VERSION     DAOPMW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41914_length_2908_cov_8.130389"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLU11_14490"
     CDS             complement(<1..206)
                     /locus_tag="PLU11_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HPN60392.1"
                     /translation="MPAKNQLLNQHLMWRAGFGPAVEQLGDLSRYTPQQIYKALVKAS
                     GRKTEYIDVADDFLKGLMMGIEEVG"
     gene            329..1372
                     /locus_tag="PLU11_14495"
     CDS             329..1372
                     /locus_tag="PLU11_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HPN60393.1"
                     /translation="MKLSAYKYLSPFIIYAGAWHSFSTTGWQIWLPLLWAWVFIPVLE
                     LFIKPSAGNMTAAEEELAKNDPTYDILLYGIVILQYVMLVKFLFSMKDPDMSLVDTAG
                     RIWVMGLLCGVFGINVGHELGHRVNVFEQTLAKALLLTSLYMHFFTEHNKGHHKRVAT
                     PDDPSSARYGEMIYTFYFRTILFSYLSAWHIANDELKKKGSPVISLKNEMIQFHLIQA
                     MFVGLIFWQFGGLTTLYFLASAGIGILLLETVNYIEHYGLQRKSTGEGKYERAMPEHS
                     WNSDHVIGRLMLFELSRHSDHHYMASRKYQVLRHHDHSPQMPTGYPGMMLLSLVPPLW
                     FRVMNQKIKKLNA"
     gene            complement(1373..1582)
                     /locus_tag="PLU11_14500"
     CDS             complement(1373..1582)
                     /locus_tag="PLU11_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60394.1"
                     /translation="MEADLKGDPVKGKQLFSAGCKRCHGLPEVGQFTRSRWEAVLPGM
                     IRNAGLDGQEAADIREYINTNLIKN"
     gene            1844..2812
                     /locus_tag="PLU11_14505"
     CDS             1844..2812
                     /locus_tag="PLU11_14505"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HPN60395.1"
                     /translation="MKRSLVLLAGSLLFMMSCNKSGSTNEDSPADDLPATQRHCAAAD
                     VLADELKADPSLGQRMAEIETFTQQVIAHPEQFRLVNGVIEIPVVFNVLYRTAAQNVS
                     LAQLQSQIDVLNEDFAATNADHNQTSTYNTVKAGDIGVRFVLDAVNRRSTTKTSWSTN
                     NAMKKSAQGIAPTSPTTKLNIWVCNMGGGILGYAQFPGGASATDGVVLDDNATGRTGT
                     AAAPFNKGRTATHEVGHWMNLRHIWGDATCGNDQVGDTPLHNTANYGCPAAGHTSTCT
                     GTPVEMTMNYMDYSDDACMYMFSVGQRSRMLAIFASGGARNSFAQP"
BASE COUNT          768 a          688 c          662 g          790 t
ORIGIN      
        1 cccacttcct caatacccat catcaggcct ttgagaaaat catcggccac atcaatatat
       61 tccgttttcc ggcccgaggc ttttaccagg gctttataga tttgctgagg ggtgtacctg
      121 gacagatcgc ctaattgttc cacggcgggg ccaaacccgg ccctccacat caggtgctgg
      181 ttcaataact gattctttgc cggcatgtgt gtattattta agtatcagac tcttcagaag
      241 ctgtttcgtt taaaaaggat gcgtaataaa ggtagtaacg cccggaaact tatcttattt
      301 ttagtaccgg attgttaaaa ttaaccatgt gaaattaagc gcctacaaat acctctctcc
      361 ttttatcatt tatgccggtg cctggcattc cttttcgacg accggctggc agatctggtt
      421 acccctttta tgggcctggg tttttatccc cgttctggaa ttatttataa aaccatcagc
      481 gggtaatatg actgccgctg aagaagaact ggccaaaaat gaccccacct atgatatcct
      541 gctatatggt attgtgattt tgcaatatgt gatgctcgtg aaatttctgt tttccatgaa
      601 ggatccggat atgtccctgg ttgacaccgc cggacggatc tgggtgatgg gtttgctttg
      661 cggggtgttc gggatcaacg taggccatga actggggcac cgggtgaatg tttttgaaca
      721 gacccttgcc aaagctttat tgctgacctc cctgtatatg cactttttta ctgaacataa
      781 taaggggcat cataaaagag ttgccacacc cgatgatccc agttcagcca ggtacgggga
      841 aatgatttat accttttact tccggaccat cctattcagc tacctgagtg cctggcatat
      901 tgccaatgat gaattgaaga aaaaaggcag ccctgttatt tccttaaaaa atgagatgat
      961 acagtttcac ctgatacagg cgatgtttgt gggtcttatc ttctggcaat tcggtggatt
     1021 gaccacactg tatttcctgg cctcagccgg cattggtatc ctgttgcttg aaaccgttaa
     1081 ttatatagag cattatggat tgcaacgcaa atccaccggg gaaggaaaat atgaacgggc
     1141 catgccggaa cacagctgga acagcgacca tgtaatcggc aggctgatgt tatttgaact
     1201 gagccgtcat tcggatcatc attatatggc cagccgtaaa taccaggtgt tgcggcatca
     1261 tgatcattcc ccccagatgc cgaccggcta tccgggaatg atgttgttgt cactggtccc
     1321 tcctttatgg ttcagggtaa tgaatcagaa aataaagaaa ttgaatgcct gatcagtttt
     1381 tgatcagatt cgtattgatg tattcccgta tatctgccgc ttcctgtcca tccagtcccg
     1441 cattacggat catccctggg agtacggcct cccaacggct gcgggtaaac tggcccactt
     1501 ccggcaatcc atgacaacgt ttacagccgg cactgaagag ttgttttcct ttcaccggat
     1561 cccctttcag atcagcctcc atattcaccg accttgttga aactgttcgc tcgggtatct
     1621 gcggggcagt cttacgggca caggaaacca acacaaataa gattccgaaa ataatacagg
     1681 ggtattttac cattcttaaa aataaacaat ccgtaaaact ccgatttcag ataaataaga
     1741 catcatgttg caataaaaga catccgtata ttctttatat gctaaaaaaa acgaataaat
     1801 tgtttagtca acttttttta cctcaaccca aaccaattta tttatgaagc gttcactcgt
     1861 actgcttgca ggcagcttat tgtttatgat gagctgtaac aaatccggat caactaatga
     1921 agacagtccg gccgatgatc tgccggctac ccaacggcat tgtgccgctg cagatgtact
     1981 ggctgatgaa ttgaaagctg atccgtcttt gggacaacgg atggcggaga ttgaaacatt
     2041 tacccaacag gtcattgccc atcctgaaca attcagactg gtgaacggag tcatagaaat
     2101 accggttgta ttcaatgtat tgtaccgcac tgcggcacag aatgtatcgt tggcccagtt
     2161 acaatcacag atcgatgtac tgaatgagga ttttgccgct accaatgcgg atcataatca
     2221 gacttctacg tataatacag tgaaagctgg tgatatcgga gtacggtttg tactggatgc
     2281 ggttaaccgc cgttctacca caaaaaccag ctggagtacc aacaatgcca tgaagaaaag
     2341 tgcccagggt attgcgccca caagcccgac taccaaactc aatatctggg tttgtaatat
     2401 gggcggcggt atcttaggtt atgcccagtt ccccggtggt gcttcggcca ccgatggtgt
     2461 ggttctggat gataatgcca ccggcagaac aggaaccgca gctgctccat ttaacaaagg
     2521 gagaactgct actcacgaag tgggccattg gatgaacctt cgtcatatct ggggcgacgc
     2581 cacctgtggt aacgaccagg taggtgatac cccgctgcat aataccgcca actatggttg
     2641 tcctgctgca ggacatacca gcacttgtac cggcacaccg gttgaaatga ccatgaacta
     2701 catggactat tcagacgatg cctgtatgta tatgttcagt gtgggacaac gcagccgcat
     2761 gctggcgatc ttcgcttccg gcggagcccg taacagcttt gcacagccct aacggatcga
     2821 acaatcctga ataataagaa cggtccggag taatccggac cgtttctttt ttatttaagt
     2881 cccgcaaaga acgcaaagag tattgctt
//
LOCUS       DAOPMW010000420         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_41974_length_2905_cov_8.909825, whole genome shotgun sequence.
ACCESSION   DAOPMW010000420 DAOPMW010000000
VERSION     DAOPMW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41974_length_2905_cov_8.909825"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..389
                     /locus_tag="PLU11_14510"
     CDS             33..389
                     /locus_tag="PLU11_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPN60396.1"
                     /translation="MAAHNELGKEGEDMAATWLAGNGYKILHRNWVQHPHEIDIIASK
                     GKFLHFIEVKTRKGNESTRPESSVNKQKFRNLKWAANAFLRQNPGNPWIQYDILAITI
                     KQWGETEYFLLEDVYL"
     gene            466..822
                     /locus_tag="PLU11_14515"
     CDS             466..822
                     /locus_tag="PLU11_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN60397.1"
                     /translation="MLNLSHKKLMVYTTALEITEEIYLLTNELPDKERYGLSSQLRRA
                     AVSVCSNLAEGAGRISKTEKKRFFEISRSSLVEIDTQIEICLRAGYLESSHLLKLEKL
                     INSTFAMLSKLITNLS"
     gene            complement(839..1357)
                     /locus_tag="PLU11_14520"
     CDS             complement(839..1357)
                     /locus_tag="PLU11_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPN60398.1"
                     /translation="MSKIPPVNLRSFKAKARHRKTAMRRFLSKLEKQAPRGLDKQIAV
                     LEKEVWQETDCLSCANCCKTMTPTYNPKDLKRISAHFKMTIPEFKKKYLKQERGGERD
                     WMNKWTPCQFLNLEDNKCSIYEIRPEDCAGFPHLSKKFKDYVHIHKQNVEYCPATYRL
                     VEKIQGKLEARS"
     gene            1460..2128
                     /locus_tag="PLU11_14525"
     CDS             1460..2128
                     /locus_tag="PLU11_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60399.1"
                     /translation="MKTLFAILAILVMAASVSCNNNSAPAPKLFCDTVCLKDTIKFTG
                     TDQKKPTVFITAKDCRADSIIWSYDGMGADRKTGFTYLLNATVNINKDFIRTYFKNSE
                     VAYVLFNDCQTGRGFQIKLPYDKSQNFSLKSSGINSFDPKFSIAENLMVNTDRGNLYV
                     EDMSTGKKAMMTFGEKLEIDYDALHDHIDSVNVTNERVWVKILSNKEWITKEKKLTPA
                     DWKD"
     gene            2346..>2905
                     /locus_tag="PLU11_14530"
     CDS             2346..>2905
                     /locus_tag="PLU11_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPN60400.1"
                     /translation="MWLPYKKGFKAYLQLEKSLSDHSVEAYLRDLEHFTSYLLEKNKA
                     INPEAVTLKDLQGFIKWISELGMTGSSQARMISGIKAFYKYCLLESICKKDPTLLLEA
                     PKLKRALPDVLSFEEIESMINQIDLSRPEGPRNKAILEAMYSCGLRVSEVVGLKISHL
                     YLDLGFIRVTGKGDKERLIPIGTNAI"
BASE COUNT          851 a          667 c          648 g          739 t
ORIGIN      
        1 tgtgttggtt taacttactg taaagataag ttatggcagc gcacaacgaa ctcggaaaag
       61 aaggcgaaga tatggccgct acctggctgg ccggcaatgg gtataaaatc cttcaccgaa
      121 actgggtaca gcacccgcat gaaatcgata tcatcgccag caagggaaaa ttcctgcact
      181 tcatcgaagt caaaacccgt aagggcaacg aatccacccg cccagaaagc agcgtgaata
      241 aacagaaatt cagaaacctc aaatgggccg ccaatgcctt cctgcggcaa aaccccggaa
      301 atccatggat acagtacgat attttagcga ttaccataaa gcaatggggg gagacggagt
      361 attttttgct ggaggatgtg tatttgtgag aggctagagg ctagaagcta gaggctagaa
      421 gggaacggcc cctgctaaga ataattaatt aaataaatct gaactatgct gaatttaagt
      481 cataagaaat taatggtgta tacaactgcg cttgaaatta cagaggaaat ctatcttttg
      541 accaatgaac tgcccgataa ggaacgatat ggtctatcat ctcaattacg gcgagcagct
      601 gtttctgttt gcagcaatct ggcagaagga gctggcagaa tttccaaaac tgagaaaaaa
      661 cgtttctttg aaatttcccg tagctcccta gttgaaattg atacccaaat tgaaatatgc
      721 ctgagggctg gctacctcga atcctctcat ttgttgaaat tagaaaaact gataaattcc
      781 acatttgcaa tgctgagcaa acttataacc aatctttctt agaaggtcct tctcgcctct
      841 agcttctagc ctctagcttt ccctggattt tctctaccaa cctgtacgtc gccggacaat
      901 actccacatt ctgcttatgt atatgaacat aatccttaaa cttcttgctc aggtgcggga
      961 agccggcaca gtcctccgga cggatttcgt agatggaaca cttgttatcc tccaggttga
     1021 ggaactggca gggtgtccac ttattcatcc agtcgcgttc cccgccccgc tcctgtttga
     1081 ggtatttttt cttgaattcg ggaatggtca ttttgaaatg tgctgaaatc cgcttgagat
     1141 ctttgggatt gtaagtaggc gtcatggtct tgcaacagtt ggcgcaggat aaacaatccg
     1201 tttcttgcca gacttctttc tccagtacgg caatctgttt gtccagacca cggggggcct
     1261 gtttttccag tttggaaagg aagcggcgca tggctgtttt ccggtgccgg gctttggctt
     1321 tgaatgaccg gagattgaca gggggtattt tactcatggg cccgaagata atgattgagc
     1381 ctgttcatcg tgcagggcca aaagaatttt cattcacagc aggtcattaa ctatttttgc
     1441 aggaatcaaa tcaacagaca tgaaaaccct tttcgcaatc ctggccattc tggtaatggc
     1501 ggcttctgtt tcctgtaaca acaattcagc ccccgctccc aaactctttt gcgataccgt
     1561 ctgcctcaaa gacacgatca aatttaccgg caccgatcag aaaaaaccta ccgtattcat
     1621 taccgcgaag gactgccggg ctgacagtat tatctggagt tatgacggca tgggcgccga
     1681 ccgtaagacc ggctttacct atctcctgaa tgccaccgtg aatatcaata aggattttat
     1741 ccgcacttat tttaaaaaca gtgaagtagc ctatgtactc tttaacgatt gccagaccgg
     1801 acgcggattc cagatcaaac taccttacga caaaagccaa aacttcagcc tgaagagcag
     1861 tggcatcaac agctttgatc ccaagttctc cattgccgaa aacctgatgg ttaataccga
     1921 ccgtggaaac ctctacgtgg aagacatgag caccggtaaa aaagccatga tgacctttgg
     1981 tgaaaagctg gagatcgact atgacgccct ccatgaccat atcgattccg ttaacgtaac
     2041 caacgagcgt gtatgggtaa agatcctttc caacaaagaa tggatcacca aagaaaaaaa
     2101 gttaacaccg gctgattgga aagattaaaa actaaatata actcctgatt taaaaggcga
     2161 caactttcag tacaatactg caggatgtcg ccttttattt ttgaacaact gttggccctg
     2221 cgggcggcag ctaaaaggac taagagatgt aaaatgagaa aaaagtgggc gtgaaaatag
     2281 taatatgctc actaaccttt gaaacccctt aaacctttta aacttcttaa acctcttctt
     2341 cccccatgtg gctcccctac aaaaaaggct tcaaagcata tctccagctc gaaaaatccc
     2401 tttcggatca ttcggtggaa gcctatttgc gggacctgga acatttcacc agctacctgc
     2461 tggaaaaaaa taaggccatc aatccggagg cggtgaccct gaaagacctg cagggattta
     2521 ttaaatggat cagtgagctg ggaatgacgg gcagttcaca ggcccggatg atttcaggca
     2581 tcaaggcctt ttataaatat tgcctgctgg aatcgatttg caaaaaagac cccaccttat
     2641 tattggaagc accaaaactg aagcgagccc tgcctgatgt attgagtttt gaagagattg
     2701 aaagcatgat caaccagatc gacctgagcc gtccggaagg tccccgcaat aaagccatcc
     2761 tggaagcaat gtatagttgc ggactccggg tgagtgaagt ggtgggactg aaaatttctc
     2821 acctctacct cgaccttggt ttcatccggg ttacaggtaa aggtgataaa gaaagactga
     2881 ttcccatcgg gacaaatgcc attaa
//
LOCUS       DAOPMW010000421         2893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_42226_length_2893_cov_4.751938, whole genome shotgun sequence.
ACCESSION   DAOPMW010000421 DAOPMW010000000
VERSION     DAOPMW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42226_length_2893_cov_4.751938"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLU11_14535"
     CDS             complement(<1..437)
                     /locus_tag="PLU11_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPN60401.1"
                     /translation="MNRSFYLNKIVYYIAGAAGILYALSYFLPALYRIAGLMLVLLGA
                     AVLIDFILVYSKKNGMAAKRELPDRFSIGDQNRVRIRLTNHYAFTVKTSIIDELPIQF
                     QERNWIRKLYLSSDQPAELDYQLRPQTRGEYTFHDINVFVHAPL"
     gene            complement(455..1432)
                     /locus_tag="PLU11_14540"
     CDS             complement(455..1432)
                     /locus_tag="PLU11_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPN60402.1"
                     /translation="MEEPIFQQRTDLSALNAAVMAIRSEIKKIIVGQDEMVKLIIAAL
                     LADGHVLIEGVPGVAKTLTAKLVAKSVNAGFSRVQFTPDLMPSDVLGTPVFNPKEASF
                     EFKKGPVFSNIILVDEINRAPAKTQSALLEIMEERQATVDGKTYPMASPFMVLATQNP
                     VEQEGTYRLPEAQLDRFLFKIVVPYPTEAEEMNILSQFHQMGNTSALDMVQPALNAEQ
                     INALRKQIKLQVMEEKLLQFVARLVHQTRNNKSIYLGASPRASLSIMNASKAIAAMQG
                     RDFVTPDDILTVAIPVLRHRIILSPDKEMEGISEDDVIRQIIQGMDVPR"
     gene            complement(1435..2682)
                     /locus_tag="PLU11_14545"
     CDS             complement(1435..2682)
                     /locus_tag="PLU11_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60403.1"
                     /translation="MKRFIPYILVAVLAMAIIVLYVSGKSDQKRVFDDRISLKRQDKI
                     PYATFVAYQQLPQLFPEARIRITRKEPGFWDSVSMYDNRQAYICITDRFAADEGEMNK
                     LLEFVRGGNDVFVSARYISSTVDEFLRCGSNSPDFFTNYGGEQEDLKTRFTLNTPPFT
                     DTVHYQYPGKTFHSYFSYVDKSTTQVLGFDDNGHPNFIRMKAGFGNFYMHLEPLAFSN
                     YFLLHEKNMGYYEKALSLIGPDVKSIVWDEYFINKKLSEGESPKQKGWMTVLMNMKNA
                     EGEKPFRAAFWLFILLLILYVIMEMRRRQRYIPVVRKPSNDSLDFVKTIGRLYYDRSD
                     HRNLCRKMSAYFLEFVRTNYKLPTGMLNDDFIRLLHFKSGVAEKEITEIVHFISQLDQ
                     GANINERQLAAFHKRLESFYQKA"
     gene            complement(2687..>2893)
                     /locus_tag="PLU11_14550"
     CDS             complement(2687..>2893)
                     /locus_tag="PLU11_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60404.1"
                     /translation="HMTEKNMIRYQQDKTNMDYLMQLHNTAYYTDFFRVTLHYEYSWY
                     GRFAVSQEAYRLITRDVEQFENRS"
BASE COUNT          748 a          710 c          648 g          787 t
ORIGIN      
        1 agcggcgcat gtacaaacac attgatatca tgaaaagtat attctcccct ggtttgaggc
       61 cgtagctggt agtcgagctc cgcaggctga tcagatgaca aatacagttt acggatccaa
      121 ttacgttcct ggaactggat cggcaactca tcaataatac tggtcttaac ggtaaatgca
      181 tagtggttcg tcagccggat cctgacccgg ttctgatcac cgatactgaa ccggtccggt
      241 agttcccttt tggcggccat accgtttttc ttactgtaca ccaggataaa atcgatcagc
      301 acagcggctc ccagtaaaac cagcatgagc cctgcgatcc ggtaaagggc cggcaggaaa
      361 taactcaggg catacaggat tcctgccgcc ccggcgatat aataaacaat tttatttaag
      421 tagaatgatc ggttcacaat agtacattat tcgtttaccg gggcacatcc atcccctgga
      481 tgatctggcg gatcacatca tcttcactga tgccctccat ttctttatcg ggcgacagga
      541 taatccggtg gcgtaaaacc ggtatggcca ccgttaaaat atcatccggt gttacaaaat
      601 cacgtccctg catagccgca attgctttgg aagcattcat aatcgacagc gaggcacggg
      661 gtgaggcgcc gaggtagatg gatttattat tccgggtctg atgcaccagt ctggccacaa
      721 actgcagcag tttttcttcc atgacctgca acttgatctg tttccgcagg gcattaatct
      781 gttccgcatt gagtgccggt tgcaccatat ccagcgcgga ggtattgccc atctggtgaa
      841 actggctcag gatattcatc tcctctgctt ccgtgggata tggcaccacg atcttaaata
      901 aaaaacggtc aagctgtgcc tccggtaagc ggtaagttcc ctcctgttcc accggattct
      961 gtgtagcgag caccataaac ggagatgcca tgggataggt tttgccatct acggtagcct
     1021 gtctttcttc catgatttcc agcaaagcgg attgcgtttt ggccggagcc cggttgatct
     1081 catccaccaa tatgatattg ctgaatacgg gccccttctt aaattcaaaa ctggcttcct
     1141 taggattaaa taccggtgta cccagtacat cggaaggcat gagatccggg gtgaactgta
     1201 cccgcgagaa ccccgcgttc acgcttttag ccaccagctt tgccgtcagt gttttggcca
     1261 cccctggcac cccttcgatc agcacatgtc catcggccag cagggcggcg atgatcagtt
     1321 tcaccatttc atcctgaccc acaataattt ttttaatctc ggaccggatc gccatgaccg
     1381 ctgcgttcag ggcagaaagg tcagtacgtt gctgaaaaat tggttcttcc ataattatgc
     1441 tttttgatag aatgattcaa gtcgtttatg aaatgcagcc agctgccttt cgttgatgtt
     1501 tgctccctgg tccagctgac tgataaagtg tacaatttcg gtaatttctt tttctgccac
     1561 cccggattta aaatgcaaga gccgtataaa atcatcattg agcatgccgg taggcagttt
     1621 ataattagta cgtacgaatt cgagaaaata agcactcatc ttccggcaga gattccggtg
     1681 atcgctgcgg tcatagtaga ggcgtccgat ggttttcaca aaatccagtg aatcattgct
     1741 gggtttccgg acaaccggaa tgtagcgttg ccggcggcgc atttccataa tcacatataa
     1801 aatcagcaag aggataaata accagaaagc ggcccggaaa ggtttttccc cttccgcgtt
     1861 tttcatattc atcagcacgg tcatccagcc tttctgcttt ggcgattcgc cttctgataa
     1921 tttcttattg ataaaatatt catcccaaac gatcgatttt acatccggtc cgatcaggga
     1981 caatgccttt tcatagtaac ccatgttttt ttcatggagt aaaaaataat tggaaaatgc
     2041 caggggttcc agatgcatgt aaaaatttcc aaaacctgcc ttcatgcgga taaaattggg
     2101 gtgcccgttg tcatcaaacc cgagcacctg tgtagtgctt ttatccacat agctgaaata
     2161 ggaatgaaag gttttacccg gatactgata atgcaccgtg tcggtaaaag gcggggtgtt
     2221 cagggtaaac cgggtcttca gatcctcttg ttcccctccg taatttgtaa aaaaatccgg
     2281 ggagttgctg ccgcagcgca gaaactcatc cactgtggag gaaatatagc gggcactgac
     2341 aaatacatca ttccctcccc ggacaaattc cagcagttta ttcatttcac cttcatcggc
     2401 tgcgaaacgg tccgtgatac agatataagc ctgccggttg tcgtacatgg aaaccgaatc
     2461 ccaaaaacca ggttctttcc gggttatccg gatccttgct tccggaaata gctgtggcaa
     2521 ttgctgatag gccacaaaag tcgcataggg tattttatcc tgacgcttca gggaaatgcg
     2581 gtcatcaaat actcttttct gatcagactt accggacaca tacaggacaa tgatggccat
     2641 cgccagcact gcaacaagta tgtatggtat aaaacgtttc actttgttaa ctcctgtttt
     2701 caaattgttc cacatcccgt gtgatcagcc ggtaagcttc ctgcgaaacg gcaaaccggc
     2761 cataccaact gtattcataa tgcagggtga ccctgaaaaa atcagtataa taagccgtat
     2821 tatgcaattg catcaggtaa tccatgttag ttttatcctg ctgataacgg atcatatttt
     2881 tctccgtcat atg
//
LOCUS       DAOPMW010000422         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_42528_length_2879_cov_11.808782, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000422 DAOPMW010000000
VERSION     DAOPMW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42528_length_2879_cov_11.808782"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1386)
                     /locus_tag="PLU11_14555"
     CDS             complement(286..1386)
                     /locus_tag="PLU11_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPN60405.1"
                     /translation="MDPQLIIEKYQNAIIQIATSNGTGTGFYVKEFDLIVTNDHVVAE
                     NAEVTIAGKTFDKALSRVWYTDRKHDLAFLEAPKNIELPDLQLGTYEQMKDGDAVVAI
                     GHPFGLNYTATQGVISKVDRIRDGLKFIQIDAAINPGNSGGPLVNMSGEIIGVNSFII
                     RGGDNLGFALPVLYLREALQLYQPNKGQASTRCFSCGSVVTVSTIDSAKYCPNCGTEV
                     KLPEIPEKEVEPVGSAKIIEDILKEMGKDVRLAREGVNHWTVKEGSAKIKITYNAENF
                     FIAGDAYLCQMPADPAKIKALYQYLLQENFRTRGLVLSCVKQNIVLSRIIYDLDMTRE
                     NGAAAFRELFQQADLYDDQLKREFGCTERLEE"
     gene            complement(1402..2724)
                     /locus_tag="PLU11_14560"
     CDS             complement(1402..2724)
                     /locus_tag="PLU11_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60406.1"
                     /translation="MFDKLFGWGKKKPAEAQEPAVVFGRYSDNNKPLSKVNRWTDADN
                     LFKEKKYPESLDAFFDYLRDDVAGNVVYERNGSEGRFELSQGSKIVRGSFNGSLVNAE
                     VTLARMPQPSVPVMRRLLEMNFNLYYSRYALEADRLCMCFDSEATTASPSKLYYGLKE
                     LSTKADKQDDLLVQDFSSLETTDDEHVTPLTDTEKEVKYTFFQQWIGETLDTIDGLDA
                     DKFSGAVAYLLLSLSYRIDYMIAPEGKLLNQLERVIAIYFKKDERPVTEKNRDMAEEF
                     RKLKALSKEEVIGYLFRSKYTFSIVAPQPYKTISDSIHAANQNMVWYRDNNYPAIASH
                     ITEYGISFCQYSYSLPKAITELFHLFMMVNYPDYFSALGFSTKYYDAASNQFQQQAIT
                     DKIYAIQQSWKEKYPLMDFKTANLRFDSLVNFNLTFTNEMEFLNMEPK"
BASE COUNT          737 a          684 c          665 g          793 t
ORIGIN      
        1 aggtgatggt tttcattgta tcaagattta cagagagtaa tgtacggaat agtttggcca
       61 aatgcaagag aacgggaatg agtaatgagt aatcaataat gagtagcaat ggaagactca
      121 gaataaatgg taaagtctga aaccgagtgt ttttgtatat acaatgcact cattatttgg
      181 tgtttatact gatttttacc gtttattttt tatatttctt cattgttttg gtgtgtcttt
      241 tcaggcaggc tcttcccggg ctactcatta ctgattactc attactcatt cttccaaccg
      301 ctccgtacac ccaaactccc gcttcagctg atcatcataa agatccgcct gttggaagag
      361 ctcgcggaag gcagcggcgc cgttttcacg ggtcatatcc agatcataga tgatgcggct
      421 taagacaatg ttttgtttaa cgcaactgag gaccaacccg cgggtgcgga agttctcctg
      481 cagaaggtat tggtagagcg ccttgatctt tgcaggatct gcgggcatct ggcagagata
      541 cgcatcgccg gcgataaaga aattctccgc attataagta atcttgatct tagccgaccc
      601 ttcctttacc gtccagtgat tcaccccctc ccgggccagc cttacatcct tgcccatctc
      661 ttttaatatg tcctcaataa tcttggccga ccccaccggt tccacttctt tctcgggaat
      721 ctcgggcagc ttgacctccg taccacagtt ggggcaatat ttagcggaat caatagtaga
      781 aacagtgacc accgagccgc aactgaaaca acgggtggag gcctggcctt tattcggctg
      841 gtataattgt aaggcttccc gcaggtaaag caccggcagg gcaaaaccca ggttatctcc
      901 gccacggatg ataaatgagt ttacaccaat tatctccccg ctcatattca ccaatggtcc
      961 gccactgttg ccgggattga tggctgcatc gatctgtata aacttaagtc cgtcacggat
     1021 acgatctact ttcgagatca cgccctgcgt ggcggtatag ttcagaccaa agggatgacc
     1081 gatggcaact acggcatccc cgtccttcat ttgctcatag gtccccaact gcagatcggg
     1141 cagttcaata tttttcgggg cttcgagaaa ggccaggtca tgtttacggt ccgtatacca
     1201 gaccctcgat aaggctttat caaaagtctt accagctatc gtcacttccg cattctccgc
     1261 caccacgtgg tcattggtta cgatcaggtc aaactctttt acataaaagc cggtgcctgt
     1321 tccgttggaa gtggcaatct ggataatcgc gttctggtat ttttcgataa tgagttgcgg
     1381 gtccattggt aataattaaa cttatttagg ctccatgttc agaaactcca tctcattggt
     1441 aaacgtgaga ttgaaattca ccaggctgtc aaagcgcagg ttggcggtct tgaaatccat
     1501 caacggatat ttttccttcc agctttgttg aatcgcatag attttatccg tgatggcctg
     1561 ttgctgaaac tgattgctgg cagcatcata gtatttagtg gaaaaaccaa gcgcggaaaa
     1621 ataatccggg taattcacca tcataaataa atgaaacagt tccgtgatgg ccttgggcag
     1681 actgtaggaa tactggcaaa aggaaattcc gtattcagtg atatgactgg caatggccgg
     1741 ataattatta tcgcggtacc agaccatatt ctgattggcg gcatgaatcg agtcactgat
     1801 cgttttatac ggctgtggtg ccacaatgga aaaagtatac ttggaccgga acagatagcc
     1861 aatcacttct tctttggaaa gggccttgag tttcctgaat tcttccgcca tatcccggtt
     1921 tttctccgtt accggtcgtt catctttttt aaagtaaatg gcgatgacac gttccagctg
     1981 gttcagcagt ttgccctcgg gcgcaatcat ataatcgatc cggtaagaaa gggaaagaag
     2041 gagataagca acagcgccgg agaatttgtc cgcatccaga ccatcaatcg tatccagggt
     2101 ttccccgatc cattgctgaa aaaaagtgta tttcacttcc ttttccgtat cagtcaaagg
     2161 ggttacatgt tcatcgtcgg tagtctccag gctcgaaaag tcctgtacca gcaggtcatc
     2221 ctgcttatcg gccttggtgg acagttcttt cagaccgtaa tagagtttac tgggactggc
     2281 cgtggttgcc tcgctgtcaa agcacataca gagccggtcg gcttccaggg catagcggct
     2341 gtaataaagg ttgaagttca tttcaagcag gcggcgcatc accggtacag agggctgggg
     2401 catccgggcc agggtcactt ccgcgttcac cagggagcca ttaaaactgc cccggacgat
     2461 tttggagccc tgactgagtt caaaacggcc ctcggaaccg tttcgttcat atacaacatt
     2521 tccggccaca tcgtcccgca ggtagtcaaa aaaggcatcc aggctttcag ggtacttctt
     2581 ttctttaaaa agattatccg cgtcggtcca gcggtttacc ttggacaacg gcttattatt
     2641 atccgaataa cggccgaaaa cgaccgctgg ttcctgtgct tccgctggct ttttctttcc
     2701 ccagccaaat agtttatcaa acatccggtg aattttggat caggcaatat taaaacaaga
     2761 tagggtaaga cgggcagatt gtcaatgaaa tccgggatga aaacaagtat tccacagcgg
     2821 cgacgaattg attgccgtat gttaaaaatc agtagtttcg ccccattcaa ttcatatta
//
LOCUS       DAOPMW010000423         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_42767_length_2867_cov_7.578592, whole genome shotgun sequence.
ACCESSION   DAOPMW010000423 DAOPMW010000000
VERSION     DAOPMW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42767_length_2867_cov_7.578592"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLU11_14565"
     CDS             <1..323
                     /locus_tag="PLU11_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPN60407.1"
                     /translation="VLLTGSKLGNDARERIQIMTSTNNGFEIAEKDLELRGPGEIEGT
                     RQSGALNFRLASIVQDRDLLEIARNMAAALLEKDPDLVSAENLQLKKFLHYRKGPVQW
                     SKIA"
     gene            426..2798
                     /locus_tag="PLU11_14570"
     CDS             426..2798
                     /locus_tag="PLU11_14570"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPN60408.1"
                     /translation="MTLKNLFTIAVTLLAVCTLSAQGNIEFIENKGQWDERVRYKGDV
                     SNGAFFIRNGGFTVVQHNPADLQRLSQFKHGLSAEGKMIGAPPPVTVRSHAWNVDFMG
                     ASTGASVIPDKPIPTYNNYFIGSNPSKWAADCKIFQGITIKNVYPGVDVRYYTDRGTL
                     KYDILVQPGADPSKIALKYDGVDKVQVKNKELVVSTSLAEFKESAPFTFQPSVTGRTE
                     VNCKYSVTGNVVRFEIKEYDRTKQLVIDPSIVFCSFSGSRSDNWGFTATYGPDESLFG
                     GGIVFGSTGSGFPVSPGAFETTYQGGEFDIGIIKLSPNGNNRIYATYIGGNGKDQPHS
                     LIVDPAGNLVLAGRTNSSDYPTINNGSAGGAGFDIVVTKLNAAGTALLGSRRIGGAGN
                     DGVNISETRGLNSLQRNYGDDGRSEVILDAAGNVYVASSTQSSDFPGTAGFFQQTFGG
                     GIQDGVVLKLSPDLGTLLFSSYIGGGGNDAAYVLSISPLTNNIYVAGGTESQNLLQGT
                     QGGTLGTANHGVIDGFVAVINNTGTNVIRSTYIGTSGIDQVFGVQFDALGFPYIMGQT
                     TGNWPIQNAVFSNAGGKQFICKMQPDLSAYVYSTAFGSGGLTPNISPIAFLVDRCENV
                     YISGWGGFFSTDNAFNSAGTTGLPVTADAFKSTTDGKDLYFFVLKKNAASQLFGSFFG
                     ENNAPGTGCDHVDGGTSRFDRNGKIYQAICGNCNIGTRPFYPTTPGAWSTVNNAVGGG
                     ECNLTMLKIDMNLAGVRAGIQSTINGVPRDTAGCQPLTVDFTDTLATVSG"
BASE COUNT          723 a          672 c          722 g          750 t
ORIGIN      
        1 gtgtattatt aaccgggtca aaattaggta atgatgcccg cgagcgcatc cagattatga
       61 catcgaccaa taacgggttt gagatagctg aaaaggacct ggaacttcga ggtccgggtg
      121 aaatagaagg aacccggcag agtggcgcat taaacttccg gctggccagt atagtccaag
      181 atcgggattt gctggaaatt gccagaaata tggccgctgc cctgctggaa aaagaccctg
      241 atttggtttc ggccgaaaat ttgcagttaa agaaattcct gcattaccgg aaaggacccg
      301 tacaatggag taaaatcgcc tgaccgtccc gtgatttttt acggaaaatg acgtccggat
      361 ggcgattctt aacagaagtt ttagatccga ttctgggaaa aattcgttat tttaaagtaa
      421 ataagttgac gctgaaaaat ctcttcacca tcgccgtaac cctgcttgct gtttgtactc
      481 tctcagccca gggcaacatt gaatttatcg agaataaagg tcagtgggat gaacgggtac
      541 gatataaagg tgatgtgagc aacggcgcct tctttatccg caatggcggg ttcaccgtag
      601 tacaacataa tcctgccgat ctgcaacgac tcagtcagtt taaacacgga ctttcagctg
      661 aaggaaaaat gatcggcgcc cctcctccgg tcaccgttcg ttcacatgcc tggaatgtgg
      721 attttatggg ggcttccact ggagctagcg tcattccgga taaaccgatt cccacctata
      781 acaactattt tatcgggagt aacccctcca aatgggcagc cgattgtaaa atattccagg
      841 ggatcaccat caagaatgta tatccgggtg tggatgtccg ttattatacg gaccgcggca
      901 cattgaagta tgatatcctg gttcagccgg gtgcggatcc ttcaaagatt gccctgaaat
      961 acgatggcgt ggataaagta caggtgaaga acaaagaatt ggtggtaagt acctcattgg
     1021 ctgaatttaa agaatccgct ccgttcactt ttcagccttc tgttaccggc cggaccgaag
     1081 taaattgtaa gtatagcgtc accggtaatg tggtgcgttt tgaaataaaa gagtatgacc
     1141 gcaccaaaca actggtgatc gatcccagca tcgtgttctg ttccttctcc ggcagccgtt
     1201 ccgacaactg gggctttacc gccacttatg gtccggacga aagtctgttt ggcgggggta
     1261 ttgtatttgg tagtaccggc agcggttttc ccgtttctcc cggtgccttt gaaaccactt
     1321 accagggcgg tgaatttgat atcgggatta tcaaattatc tcccaatggc aataaccgga
     1381 tttatgccac ctatatcggg ggtaacggaa aggatcaacc gcattcactg attgtggatc
     1441 ctgccggtaa cctcgtatta gcgggccgga ccaattcctc cgattatccg actatcaata
     1501 atgggtcggc aggcggagcc ggctttgata tcgtggtcac taaactgaat gccgcaggta
     1561 cagcccttct cggttcacgt cgtatcggcg gagccggcaa tgatggagtg aatatttctg
     1621 aaacccgtgg attaaattcc ctgcaacgga attacggcga tgatggccgt agtgaagtga
     1681 ttctcgatgc cgccggaaat gtttatgtgg cttcgtccac ccaatcttcc gattttcccg
     1741 gcaccgcagg attctttcaa caaactttcg gaggtggaat tcaggatggg gtggtactta
     1801 aattatcccc cgatctgggc accctgcttt tttccagcta tatcgggggg ggcggtaatg
     1861 atgctgctta tgtgctttcg atcagtccgc ttaccaataa tatctatgtg gccggtggaa
     1921 ctgaaagtca gaatcttctc cagggaacac agggaggaac actcggtaca gcaaaccatg
     1981 gagtaatcga tggctttgta gcggtcatca ataatacagg taccaatgtt attcgttcca
     2041 cttatatcgg tacctccggt attgaccagg tattcggggt gcagtttgat gccctgggtt
     2101 ttccatatat catgggacaa accacgggta actggcctat acaaaacgcg gtattcagta
     2161 atgcaggcgg taagcagttt atctgcaaaa tgcagcctga tctttctgcc tatgtttatt
     2221 caacggcttt tggttccgga ggactaacgc ccaatatttc acccattgcc ttcctggtag
     2281 accgttgtga aaacgtgtac atctccggat ggggtggttt cttcagtacc gataatgcct
     2341 ttaacagtgc tggtacaacc ggattacccg ttaccgctga tgcatttaaa agtacgaccg
     2401 acggaaaaga tctttacttc tttgtattga aaaagaatgc cgcctcccag ttatttggca
     2461 gtttcttcgg cgaaaataat gccccgggta cgggatgtga tcacgtggat ggaggtacca
     2521 gccgtttcga ccgcaatgga aaaatttacc aggcgatctg tggcaactgt aatatcggta
     2581 ccagaccctt ttatcccact acacccggcg cctggtctac cgtgaataat gcggtgggtg
     2641 gcggagagtg caacctgacc atgctcaaga ttgatatgaa tctggcgggt gtacgggccg
     2701 gaatacaatc cacgatcaat ggagtacccc gggatactgc cggttgtcag ccactcacgg
     2761 ttgactttac tgataccctt gcaaccgtga gtggctgaca accggcagta tcccggggta
     2821 ctccattgat cgtgaattgt attccggccc gtacacccgc cagattc
//
LOCUS       DAOPMW010000424         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_42768_length_2867_cov_6.650427, whole genome shotgun sequence.
ACCESSION   DAOPMW010000424 DAOPMW010000000
VERSION     DAOPMW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42768_length_2867_cov_6.650427"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="PLU11_14575"
     CDS             <1..1756
                     /locus_tag="PLU11_14575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60409.1"
                     /translation="ALDGKGYSLMHMGKLTQSLQCFLEAVKIAEDPKNDNKTFYRKTK
                     AEASKFRLTTLANIHHDWGHLMGSINLDEQIFHYRQTEKIASEIKDSTMLAFVNMNLG
                     HVYTDKGLYDTALVMEQHAAKLFIRSGDKRYLGLVYTITGDIYLNKKERGKALEYYHK
                     AIQTSQQENNLSNLNYNYNRLSDYYFNEQNNDSCLFYARKGMEGSLSMGNMNMERWYT
                     LMYKGYRLRNDRDSVLKYQELALIAKDSSYKKEVDNLTELQRTSFAEQQRMQELEQEK
                     LNSKNKLRNYALLGILAIFLIAGALLFRNNRQKQKAKTEIELAYAELKATQSQLIQSE
                     KMASLGELTAGIAHEIQNPLNFVNNFSEVNTELIDEMKEEFIKGNTTEAVQLAEDIKQ
                     NNEKISFHGKRADSIVKGMLQHSRNSSGQKELTDINNLADECLRLSYHGFRAKDHSFN
                     SKTETHFEEGLPHIQVAPQDIGRVLLNLITNSFYSVALKRKEADDGYEPMIEVSTKNL
                     GNAIEISVRDNGNGVPQKVLDKIFQPFFTTKPTGEGTGLGLSMSYEIITKGHGGQLVV
                     DTKEGAYAEFRITLPFKH"
     gene            1761..2138
                     /locus_tag="PLU11_14580"
     CDS             1761..2138
                     /locus_tag="PLU11_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN60410.1"
                     /translation="MMKILVVDDETDVQTLFEQRFRREIKNGEMHFDFAFSGEQALKY
                     LNEHEQEAVLILSDINMPGMSGLELLGQIKSKYFKPPPVVMMITAYGDAENFNTAKQL
                     GADDFLTKPVDFTELKEKLKTLI"
     gene            2138..>2867
                     /locus_tag="PLU11_14585"
     CDS             2138..>2867
                     /locus_tag="PLU11_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN60411.1"
                     /translation="MAKILVVDDEADLEVLIRQKFRQKIRENLYQFVFAANGVHALEQ
                     LTQHHDVDVVLSDINMPEMDGLTLLTKLGEQNGLLKSVIVSAYGDMDNIRTAMNRGAF
                     DFITKPVNFEDLELTMEKTIKHVTQLRETMKAIKENNILRMYVDETVLNFMGGREFES
                     SLAANETVEASIAFFDICGFTSISENETPDKVVRLLNSYFDVMVKEIIAQGGYIDKFM
                     GDAVMAVFRGDYHLDRAIDAALAVR"
BASE COUNT          912 a          581 c          716 g          658 t
ORIGIN      
        1 tgctttggat ggcaaagggt attcattaat gcacatggga aaactgacgc agtccctgca
       61 gtgttttctg gaggcggtaa aaatagcgga ggacccgaag aacgataata aaacatttta
      121 tagaaaaaca aaagcggagg cgtcaaagtt caggctgacc acactcgcca atattcatca
      181 cgactgggga cacctgatgg gcagtatcaa tctggatgaa cagatatttc attacaggca
      241 aacagaaaag atcgccagtg aaatcaaaga ttcaaccatg ctggcttttg taaatatgaa
      301 cctgggtcat gtatatacag acaagggact atatgacaca gcccttgtga tggaacagca
      361 tgcagcaaaa ctgtttatcc ggtcaggtga caagcgttac ctgggacttg tatatacgat
      421 caccggggat atctatttga acaagaagga gcgggggaag gcattggaat attatcataa
      481 agcgatacaa acgtcgcaac aggagaataa tttatcaaat ctgaattaca attataacag
      541 gttaagtgat tattatttta atgagcagaa caacgattcc tgtctattct atgcccggaa
      601 aggaatggag ggatctctgt caatgggtaa tatgaacatg gagagatggt acacgctgat
      661 gtataaaggg tatcggttac ggaatgacag ggatagcgta ttgaaatacc aggagctagc
      721 cctgatagca aaagatagtt cttacaaaaa agaggttgac aatcttactg aattgcaacg
      781 aacctctttt gccgaacagc aaagaatgca ggagttggag caggagaagc tgaactcaaa
      841 aaacaagttg agaaactatg ctttacttgg catcctggct attttcctga tcgcgggggc
      901 actgcttttc cggaataatc ggcaaaaaca aaaggccaaa acagaaattg aactggccta
      961 tgctgaatta aaagccaccc agtcccagct gatacaaagc gaaaaaatgg ccagcctggg
     1021 agagctaacc gccggtatcg cccatgaaat acaaaacccg ctgaacttcg tcaataactt
     1081 ttccgaagtg aataccgagc tgattgatga aatgaaggag gaatttataa agggaaatac
     1141 tacagaagcg gtgcagctgg cagaggatat aaaacaaaac aacgagaaga tctcttttca
     1201 tggtaaaagg gccgactcaa ttgtaaaagg aatgctgcaa cacagccgga acagcagcgg
     1261 tcagaaggaa ctgacggata tcaataacct ggccgatgaa tgcctgcgac tgagttatca
     1321 cgggttcagg gccaaggatc attcgtttaa ttccaaaacg gaaacccatt ttgaagaagg
     1381 cttgccccat atacaggtag cgccacagga tattggacgg gtactcctga acctgatcac
     1441 caactcattt tattccgtgg ccttgaaacg caaagaagcc gacgatgggt acgagccgat
     1501 gatcgaagtg tctaccaaga acctgggaaa cgccattgaa ataagcgtac gggacaacgg
     1561 caacggggtg ccacaaaaag tactggataa aatattccag ccttttttca ccaccaaacc
     1621 taccggcgaa ggaaccgggc tggggctcag tatgagctac gaaatcatca ccaagggcca
     1681 tggtggtcag ttggtggtgg ataccaaaga aggagcatat gccgaattca gaataacatt
     1741 accatttaaa cattgaacca atgatgaaaa tcttagttgt agatgacgaa acagatgttc
     1801 agaccttatt tgaacaacga ttccgccggg aaataaaaaa cggggaaatg cattttgatt
     1861 ttgccttttc tggcgagcaa gcgctaaaat acctgaatga gcatgaacag gaagccgtgc
     1921 tgatcctctc cgacatcaat atgccgggga tgagcgggct ggaactgctg gggcagatca
     1981 aatccaagta cttcaaaccc ccgcccgtgg tgatgatgat tactgcttac ggtgacgctg
     2041 aaaacttcaa taccgccaag caattggggg cggatgattt tctgaccaaa ccggttgact
     2101 ttactgaatt aaaagaaaaa ttaaaaacac tgatctgatg gctaaaatac ttgtagtaga
     2161 cgacgaagcg gatctggaag tgttgatccg gcagaagttt cgccagaaaa tccgggaaaa
     2221 tctgtatcag tttgtatttg ccgccaacgg ggtgcatgca ctggaacagc tgacccagca
     2281 ccacgatgtg gatgtggtgc tgagcgatat caatatgcct gaaatggacg gactgaccct
     2341 gctcaccaaa ctgggcgagc aaaacgggtt gctgaagtcc gtgatcgttt ccgcctacgg
     2401 ggatatggat aatatccgta ccgcgatgaa ccggggcgct tttgatttta ttaccaagcc
     2461 ggtcaatttt gaagacctcg aactgacgat ggaaaaaacc atcaaacatg tcacccagtt
     2521 gcgggaaacg atgaaagcta tcaaggagaa caatatcctg agaatgtatg tggatgaaac
     2581 ggtactaaac tttatgggcg gtcgtgaatt tgaatcttcc ctggctgcca atgaaacagt
     2641 ggaagcctcc attgcgtttt ttgatatttg tggatttact tccatcagtg aaaatgaaac
     2701 ccccgataaa gtggttagac tcctgaacag ctattttgat gtgatggtca aggagattat
     2761 tgcccagggt ggttatattg ataagttcat gggtgatgcg gtaatggcgg ttttccgggg
     2821 tgattaccac ctggacaggg ccatcgatgc ggcactggcg gtccgga
//
LOCUS       DAOPMW010000425         2849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_43150_length_2849_cov_6.894775, whole genome shotgun sequence.
ACCESSION   DAOPMW010000425 DAOPMW010000000
VERSION     DAOPMW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43150_length_2849_cov_6.894775"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="lpdA"
                     /locus_tag="PLU11_14590"
     CDS             <1..1353
                     /gene="lpdA"
                     /locus_tag="PLU11_14590"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPN60412.1"
                     /translation="AIRASQLGFKTAVIEKESLGGICLNWGCIPTKALLKSAQVIEYI
                     KHAKDFGIEATGTPDFGAVVKRSRGVADKMSKGVQFLMKKNKIDVIMGFGKLIAKGKL
                     EVTDKDGKTQVVEAKHIILATGGRSRQLPTMPIDGKKIIGYREAMVLPEMPKSMIVVG
                     SGAIGVEFAYFYNSMGTKVTIVEFLPRIVPVEDEDISKELEKQYKKKGIDIMTNSEVT
                     AVDTTGAGVKAKVKTATGEVTLEADVLLSAVGVAANIEGIGLEALGVKTEKGRILVDK
                     YSQTNIPGIYAIGDCSPGQALAHVASKEGIVCVEAIAYNEKKYNHLPEAIDYNNVPGC
                     TYCVPEIASVGYTEKQAKDAGYEIKVGKFPLSASGKASAAGHTEGFIKVIFDAKYGEW
                     LGTHMIGYNVTELIAETVTARKLETTYHEVLNAIHPHPTISESIKDAIEVAYGEAIHI
                     "
     gene            complement(1552..2781)
                     /locus_tag="PLU11_14595"
     CDS             complement(1552..2781)
                     /locus_tag="PLU11_14595"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HPN60413.1"
                     /translation="MKAGRILIFNIICLLSFTRAAAQLNCVTTEHTRLQISSNPVQAN
                     TIQQAETFIRQVIANPSAGRLENGIIKIPVVIHNLYHTTSQKITDEQAAAQLDILNKA
                     FRRKNPDTVKTPAVFKSVAADCEIEFHLATSDPLRRSTTGIIRKYTPVTTWVMDDKVK
                     SSADMGDDPWDSRLYLNVWVCQMDRFAGYSTVPGSDAKLDGIVLGLSAFGAGQKTIIH
                     EAGHWMGLKHIWGDEYCGDDGVSDTPKQASYTVGCPGNVRVTCGNSPTGDMYNNYMDF
                     TNDGCMNLFTKGQKARMQAVFAAGAARNSLLNSAGLDKPMIYESPLAEEDPRWLQIKV
                     YPNPVTTTLYLDFSYDVRWIGKTIFISNLVGQSVNNVTISSKIQTIDVTNFKPGVYFL
                     AAKNDQGVSMKVKFIKL"
BASE COUNT          824 a          657 c          674 g          694 t
ORIGIN      
        1 gcaatccggg cttcccaatt aggattcaaa acagcagtga ttgaaaaaga aagcctcggt
       61 ggcatttgcc tgaactgggg ctgcatcccc accaaagccc tcctcaagag cgcccaggtg
      121 attgaatata tcaaacatgc caaagatttc ggcatcgaag ccacaggcac acccgatttt
      181 ggcgccgtgg tgaaaagaag ccggggtgtg gccgataaaa tgagcaaagg cgtgcagttc
      241 ctgatgaaaa agaacaaaat cgatgtgatt atgggcttcg ggaaactgat tgccaaagga
      301 aagctggaag taaccgataa agatggcaaa acccaggtgg tggaagccaa acatatcatt
      361 ctggctaccg gcggcagaag ccgtcagctc cccaccatgc ctatcgatgg taaaaagatc
      421 attggttacc gtgaagctat ggtactgccg gaaatgccca aatccatgat tgtggtaggc
      481 agtggtgcga ttggtgtgga attcgcctat ttctacaaca gtatgggcac taaagtaacc
      541 atcgtggaat tccttccccg cattgttccg gtggaagatg aagacatttc caaagaactc
      601 gaaaaacaat acaaaaagaa aggcattgat attatgacca acagtgaggt aacagctgtg
      661 gatactaccg gtgccggtgt gaaagcaaaa gtaaaaacag ctacaggcga agtcacactg
      721 gaggcggatg tgctattgag tgcggtaggt gtcgctgcta atattgaagg gatcggactg
      781 gaagccctgg gtgttaagac cgaaaaagga cgtatcctgg tagacaaata cagccagacc
      841 aatatccccg gtatttatgc aatcggtgac tgttccccag gccaggccct ggcacacgtt
      901 gccagcaagg aagggattgt ttgcgtggaa gccattgcct ataacgaaaa aaaatacaac
      961 cacctcccgg aagccatcga ttataataat gttcccggtt gtacctactg cgtgccggaa
     1021 atcgcatcag tcgggtatac cgaaaaacag gcgaaagatg ccggttacga aatcaaagtg
     1081 ggtaaattcc cgctgagtgc cagtggtaaa gcctctgccg ccggccatac agaaggtttc
     1141 attaaagtaa tcttcgatgc caaatacggc gaatggctgg gaacccacat gattggctat
     1201 aatgtaaccg aactgattgc ggaaaccgta acggcccgga aactggaaac cacctatcat
     1261 gaggtgctga atgcgattca cccccacccg acgatcagcg aaagtatcaa ggatgctatt
     1321 gaggtggctt atggagaagc catccatatt taagaaagaa acaagaacca aagatgccaa
     1381 gaaccaaata aacatcaagg ttcaacgttc aaatcacaaa acatacttag aggctgtttc
     1441 atggaaatgg acagcctctt tttttgtgca ttatttgatt gactggatta agagcaaaaa
     1501 aaagaccgcc tgagggcggc ctttgcgtta gtatgtattc tggttgcgac tttacagttt
     1561 gataaacttc actttcatag acactccctg atcatttttt gctgcaagga aataaacacc
     1621 cggtttaaaa ttggtcacat cgatagtctg tatttttgag ctaatcgtaa cattattaac
     1681 actttgtccg actaaattgc tgataaaaat agttttcccg atccaacgca catcatacga
     1741 aaagtccagg tataaggtcg tagtgaccgg atttggatac acttttatct gtaaccaacg
     1801 gggatcttct tccgctaatg gtgattcata gatcatcggc ttatccagtc cggcggaatt
     1861 gagcaaacta ttcctcgccg ctcccgccgc aaaaactgcc tgcatcctgg ccttctgtcc
     1921 cttagtaaac agattcatac aaccatcgtt ggtgaaatcc atgtaattgt tatacatatc
     1981 cccggtagga ctattgccac aggtaaccct tacattgccg ggacaaccta cggtataaga
     2041 agcttgcttc ggagtatcac tcaccccatc atccccgcaa tactcgtctc cccagatatg
     2101 tttaagtccc atccaatgtc ctgcttcatg aataatcgtt ttctgacccg ctccaaaagc
     2161 tgaaagtcct agaacaattc cgtccagttt agcatccgaa cccggtaccg tagaatatcc
     2221 ggcaaagcga tccatctggc agacccagac attcaaataa agccggctgt cccaggggtc
     2281 atcgcccata tcggcagaag atttcacttt atcgtccatg acccaggtag taaccggggt
     2341 atacttacgg ataatgccgg ttgtagaccg tcgaaggggg tcagaagtag ccaggtgaaa
     2401 ctcaatttca caatcagctg caacggattt aaaaaccgcg ggcgttttaa ctgtatccgg
     2461 gtttttccgg cggaatgctt tattcagtat gtccagctgg gcggctgcct gttcatcggt
     2521 tattttctgg ctggtagtgt gatagagatt atggatgacc accggtatct tgatgatccc
     2581 gttttcgaga cgaccggcag aaggattcgc tatcacctgc cggataaatg tttccgcctg
     2641 ttgtatggta tttgcctgta ccggattgct gctgatctgc aggcgggtgt gttctgtcgt
     2701 tacacagttt agctgtgctg ccgcacgggt aaatgagagt aagcaaataa tattaaaaat
     2761 caggatcctt cccgccttca tagttccaaa ttgtaatagg tacagggctc ataaaatggc
     2821 catgagtcat tcaacgggat tggagaaaa
//
LOCUS       DAOPMW010000426         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_43205_length_2847_cov_5.448424, whole genome shotgun sequence.
ACCESSION   DAOPMW010000426 DAOPMW010000000
VERSION     DAOPMW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43205_length_2847_cov_5.448424"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2824)
                     /locus_tag="PLU11_14600"
     CDS             complement(<1..2824)
                     /locus_tag="PLU11_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60414.1"
                     /translation="MKKVLSLIACLCTLFVMESAAQVNTVEFGKNRVQYQKFKWKYYQ
                     TENFNSYFSQDGLGLGKYVAQVAEAELPSIEEFVEYGLQRRINIVIYNNYDEMQQSNI
                     GIGIDWQNTGGVTKLVNNKMIVYYDGNHDNLRRQIRQGIARNLVENLLFGDDLGEFAA
                     NQALLDLPKWLTDGYIAYAAENWNTTLDDDLRGVMLAGSYRNFYQFAFEKPMLAGHSF
                     WKYIADKYGKNKTTYFLYLARIYRNLNNASYKIAKKKFKIILRDFMTDVQNQYFKDIR
                     GRRNSPKGQLSVSEYIGKKDYLHFNANPIPRSFTYAVVEFNQGRYQVVLMENFVNKKI
                     LLKQGVRTREDDKHPNYPLLAWDGKGTRLAVLYHEEGKTKFFVYDLVNRVKLWKQTLD
                     KFDQVQDMKYMLDNNTLVLSAVRSGQSDIYVYKIDKQSFEQITNDVYDDLDPSFVAFP
                     NKTGILYSSNRPSAEAGSSDDSLPNRHFNVYLVDNWNKSEVKQISKLTDLRLGNARFP
                     SQYNTSHFTFISDENGIANRYAGFFKTERAGLDTLVFIGDEMLRNPVQKDVDSLLAEW
                     NKTDIDSVGFVSVTNDSSYVFPLTNYQSNTLETRTAGDNQQVSEVVKLGDVKLLYRLK
                     VDENTLRRRNINARPTEYMRKVIEEEKKALRKEAAVTAKTDTLNKKQQDFFNTGFEEE
                     KTDSTRKVGDVVRAEELAKESVLKKAKVYEYRPPKFFNDYLVSGFNNNVLVTRYQPYQ
                     GGNGPIQLSNNDPLNGIIRLGTSDLFEDWKIAGGFRVSTDLTNNEYIFTTQYLKKRID
                     YGFTYYRNGALYTSNVGDVKLFSNLYQGSVSYPFNKVRSLRFNMGYRSDKYVFKANKD
                     VPQTLDLEDIRQTYALARIEFIHDDAISPVLNIWKGLRWKAYMDYNFQIGKNTGTGSG
                     ENPYIMNFGFDARHYLPIYRNVSWSVR"
BASE COUNT          688 a          665 c          663 g          831 t
ORIGIN      
        1 ctcttaccga ccagctgaca ttccggtaga tcggcaggta atgacgggca tcaaacccaa
       61 aattcatgat ataggggttc tcgcctgagc cggttcctgt gtttttacca atctggaagt
      121 tatagtccat gtatgctttc cagcgtagac ctttccagat attcaacaca gggctgatgg
      181 catcatcatg aataaattca atccttgcca gtgcataggt ttgccggata tcttcgagat
      241 ccagggtctg cggtacatct ttattcgctt tgaatacata tttatcactg cgatagccca
      301 tattgaaacg aaggcttctg accttattaa aggggtagga aacggagccc tggtacaggt
      361 ttgagaataa cttcacatcg cccacattgc tggtatacaa tgcgccatta cggtaataag
      421 taaacccata gtcaatccgt tttttcaggt attgggtggt gaaaatatat tcgttattgg
      481 tcaggtcggt actcacccgg aaacctccgg cgatcttcca gtcttcaaac agatcggatg
      541 tgccaagacg gataatcccg tttagcggat cattattgct gagctggata ggtccattgc
      601 caccctggta aggctggtaa cgggtaacca atacattgtt attgaagccg ctgaccagat
      661 aatcgttgaa gaatttcggc ggacggtatt catatacttt tgctttcttc agtacggatt
      721 ccttcgccag ttcttctgct ctcaccacat cgcctacttt acgggtgctg tcggtttttt
      781 cttcctcaaa gccggtatta aaaaagtcct gctgtttttt attgagtgta tcggtcttgg
      841 ctgtcaccgc agcttccttg cgaagggctt tcttttcttc ttcaatcact ttccgcatgt
      901 attcggtcgg acgggcatta atattacgac gacgcaacgt gttttcatcc acttttaagc
      961 gatagagcag tttgacatca cccagtttca ccacttcaga tacctgctga ttatcgccgg
     1021 ccgtgcgggt ctcaagtgta ttgctctggt aattggtcag cgggaataca taggaagaat
     1081 cattcgtaac agaaacaaat cctacggagt cgatatccgt cttattccat tctgccaaca
     1141 gactgtccac atctttctgt accgggttgc ggagcatctc atccccgata aacacgaggg
     1201 tatccagtcc ggccctttct gttttgaaga atccggcata tcggttggca attccgtttt
     1261 catcactgat aaaggtgaag tgcgaagtat tatactgcga agggaaacgg gcattgccca
     1321 ggcgcagatc agtcagtttg gaaatctgtt ttacctcgga cttattccag ttatcaacga
     1381 ggtacacatt gaaatgccgg tttggcagag aatcatcgct gctgcccgct tcggcgctgg
     1441 ggcggttgga ggaatacagg atacccgttt tattcgggaa agcgacaaag gaaggatcga
     1501 gatcatcata cacatcattg gtgatctgtt cgaaactctg tttgtcgatc ttatagacat
     1561 aaatgtcgga ttgcccgctt ctcacggcac tcagcaccag ggtattgtta tccagcatat
     1621 atttcatgtc ctgtacctgg tcaaatttgt caagggtctg tttccagagt ttgacgcggt
     1681 tgacaagatc atacacaaag aattttgtct tgccttcctc atgatagagt acggcgagtc
     1741 gggtaccctt gccatcccag gccagcaggg ggtaattggg gtgtttgtca tcctcacggg
     1801 tacgaacccc ctgtttcaat aatatttttt tattgacgaa gttttccatc aataccacct
     1861 ggtagcggcc ctggttaaat tccaccaccg cataagtaaa gctgcggggt atcgggttgg
     1921 cattgaaatg cagatagtct tttttgccta tatattcact cacggataat tgtcctttgg
     1981 gtgaattgcg ccggccacgg atgtctttga aatactggtt ctgcacatcc gtcatgaaat
     2041 cacgcaggat gatcttgaat tttttcttgg caattttata cgaagcatta ttcaggttgc
     2101 ggtagatccg ggccaggtac aggaaataag tggttttatt ttttccgtat ttatccgcaa
     2161 tatatttcca gaaggagtgc ccggccagca tgggcttttc aaaagcaaac tgataaaaat
     2221 tccggtagct gcctgccagc attacgccgc gcaggtcatc atccagggtg gtgttccagt
     2281 tttcggcggc ataggcgata tagccatcgg tcagccattt gggaagatcc agcagggcct
     2341 ggttggcagc aaattctccc aggtcatctc cgaagagcag gttttcgacc aggttgcggg
     2401 caataccctg gcgaatctgc cggcgcaggt tatcgtgatt gccatcatag taaacaatca
     2461 ttttgttatt caccaacttg gtcaccccgc cggtattttg ccagtcgata ccgatgccga
     2521 tattggactg ctgcatttca tcgtagttgt tatagatcac gatattgatc cggcgctgga
     2581 ggccatattc cacaaactcc tcgatggagg gtagttcggc ttcagctacc tgggccacat
     2641 atttgcccag tcccagcccg tcctggctga aataactgtt aaagttttca gtctggtaat
     2701 atttccattt gaatttctgg tactgcacgc ggtttttacc aaactccacg gtattcacct
     2761 gggcggcaga ttccattaca aagagggtgc aaaggcaggc aataaggcta aggactttct
     2821 tcatagacta tgacttactg ttcaaaa
//
LOCUS       DAOPMW010000427         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_43404_length_2838_cov_6.195832, whole genome shotgun sequence.
ACCESSION   DAOPMW010000427 DAOPMW010000000
VERSION     DAOPMW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43404_length_2838_cov_6.195832"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..809)
                     /locus_tag="PLU11_14605"
     CDS             complement(225..809)
                     /locus_tag="PLU11_14605"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPN60415.1"
                     /translation="MEIESAWFTDEQFKSSRLKNPKIIHSQPTDSLLKPMMHRSDNFF
                     AEQSLLMVSNEYLGMMNDAKIIDTLLKTDFKDLPQKPRWADGSGLSRYNLFTPQDFVT
                     ILNKMKNEFGMERIKTIFPTGGEGTISSYYKAGNGYVYAKTGTLSGVVALSGFLYTKK
                     GKLLIFSTLVNNHQASATEVRRAVERFIESLRKY"
     gene            complement(1828..>2838)
                     /locus_tag="PLU11_14610"
     CDS             complement(1828..>2838)
                     /locus_tag="PLU11_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN60416.1"
                     /translation="GMSGKLFHAPFLQAHPGYELKAIVERHNHDSKERYPDAKLYRSV
                     EELCADPELDLIVVNTPTHLHYEQALLVLNSGKNMVIEKPFAVTTAQAEEITKLAESK
                     HLFISVYQNRRYDGDFRAVKEVIDRGWLGDLREAEIRYDRYRPAFSGKAHKEGDMPGA
                     GIIYDLSPHLVDQAIQLFGFPKALFADVWRMRPDVVANDYFEILFYYDSMRVRLKATC
                     IARESLFAYTIHGMKGSFLQQRSDLQEIKLQEGVKPSLDNWCEAPSKPDGLLHTEING
                     EVVRQETTSGPGNYMGYYDDVYKALTGAGPNPVPAADGIRNMRIIDAALVSVREKRVV
                     LL"
BASE COUNT          771 a          640 c          621 g          806 t
ORIGIN      
        1 gtgccagatg ttttttccgg gtcatcaata gttcaacccc gttggggttg gagggcattc
       61 aactgatcac ctacccccgg gttgacacct ggggctatga atggttcaac cccatctacc
      121 ttcgccaagg cttcggtgga cacgtcgggg ttagttgtaa tggacagtca tagggacatg
      181 gtttgccact caaggtttga ttccgaaacc gtggtgatgt ggttttaata tttccgcaag
      241 ctctcaataa acctttcaac cgcccttctc acctccgttg cactggcctg atgattattg
      301 accagggtgg agaaaatgag cagcttccct ttcttcgtat ataagaaacc gcttaaggcc
      361 accacgccgg agagggtgcc ggtcttggca taaacataac cgttgccggc tttgtagtaa
      421 gaactgatcg tgccttcgcc accggtcggg aaaatggttt tgatccgttc cattccgaat
      481 tcatttttca ttttattcag gatggtgacg aagtcctgcg gggtaaagag attataccgg
      541 ctgagtccgg agccatcagc ccagcggggc ttttgcggca ggtctttgaa atcggtttta
      601 agcagggtgt caatgatctt cgcatcattc atcataccca ggtattcatt gcttaccatc
      661 aggagcgatt gctcggcaaa gaaattgtcc gagcggtgca tcatgggttt gaggagtgag
      721 tcggtgggtt gggagtggat gattttggga ttctttaaac ggcttgattt aaattgttcg
      781 tcagtaaacc atgctgactc gatttccaaa aattttgcac ttgccactgg agaatttcgt
      841 gagtaagaat aatcaatccg tttattcagc gtatcctcaa tcaatacttt tggaaaattc
      901 aaatctatgc taaatggaag ttgttgttta taattgctca catttgtatc agatttcact
      961 tcgtaataat tcttttcaga gggcttttta atctctgctc tgccatttct aaatccgtaa
     1021 gaaattaaat tattcttaga aaaatatccc ggctcagaac ttacgatacc tttattgaca
     1081 cttatacata ccatattccc gtatacaggg aaataagacc tggttggtaa ataattctca
     1141 cctaagtcac caatattcca ccctcctgaa taagaaatcg tattaaccat aggtaataca
     1201 actccaaatc taattggggt agttttaaga aaattataga ctggttgaga aataaaatca
     1261 gaatgcagaa atgttggatc acctgttggt tcaattagca tacttgtatc tcctattgaa
     1321 gcaactattt cataccgcaa ccccaccaaa ctatccccca gatacttcat cgccgcataa
     1381 caagtcggga ttttggtatt gctggccggg acaaaatatt tatcaccctg gtaattgtag
     1441 aggtatttac cggtttcggg atcgtagatg gaaataccga tatgggcggt ttttaaggga
     1501 gccgcgttga ggacggtgtt tttggctgat ttggagattt tggaggaaac tgagcaggag
     1561 gtgagcaata aaaattcaaa agtaaaaagt aaaaataggg gtccgaaaga aggaaatgat
     1621 ttcatgatag ctggttgaga ttacgaaaga tcggaagaac ctgagaaagt caaaatttaa
     1681 aagtaaaaaa tccgaaagat cgcggctaac atttctgtag ccagagataa aattacaatc
     1741 agaaaatatt tttgcttttc gaagtcaaaa tgactcttca atttatatga ctgccatttt
     1801 tactttttaa ttttaacttt tgacttatca aagcaacact acacgtttct cgcgaacgct
     1861 caccaaagcc gcatcaataa tccgcatatt cctgatccca tccgctgccg gtacgggatt
     1921 cggtccggca ccggtgaggg ctttatatac atcgtcgtaa tagcccatgt agttacccgg
     1981 acctgaagtg gtttcctgtc ttaccacttc accgttgatc tctgtatgca acaggccatc
     2041 gggtttggaa ggagcttcgc accagttgtc cagcgatggt tttacgcctt cctgtaattt
     2101 gatctcctgc aaatcggagc gctgctgcag aaaactgcct ttcatcccat gaatagtata
     2161 cgcaaataaa gattcacggg cgatacaggt ggctttgagc cgcacacgca tcgaatcata
     2221 ataaaaaagg atctcgaaat aatcattcgc caccacatcc ggacgcatcc gccatacatc
     2281 ggcaaacagt gctttgggaa aaccgaataa ctgaatggcc tgatccacca ggtggggcga
     2341 caggtcatag ataatacctg ccccgggcat atccccttcc ttatgcgctt tgccactgaa
     2401 ggccggacga taacgatcat accggatctc cgcttctctt aaatccccca gccaaccccg
     2461 gtcgatcact tctttcacgg cacggaagtc gccatcataa cggcggttct gataaacact
     2521 gataaataaa tgctttgatt cagccagttt tgtaatttct tccgcctggg cggttgtcac
     2581 cgcaaatggt ttttcgatca ccatgttttt cccactgttc agtaccagca aagcttgttc
     2641 ataatgcaga tgcgtgggtg tattcaccac aatcaggtcc agctccggat cggcacagag
     2701 ttcttccaca ctacgataca acttagcatc cggatatctt tcttttgaat catggttatg
     2761 ccgttccact atagccttca gttcataccc gggatgggcc tgaaggaagg gtgcgtggaa
     2821 aagtttcccg gacatgcc
//
LOCUS       DAOPMW010000428         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_43583_length_2830_cov_7.069189, whole genome shotgun sequence.
ACCESSION   DAOPMW010000428 DAOPMW010000000
VERSION     DAOPMW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43583_length_2830_cov_7.069189"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..997)
                     /locus_tag="PLU11_14615"
     CDS             complement(404..997)
                     /locus_tag="PLU11_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60417.1"
                     /translation="MRKKMAITVLFLSGGMYFQACNKKDALPNPCLGVSYDIQYFKTE
                     SVGSSNNGSLTITSPVGDTISYQLNSAAFQSSPIFSNLAPGDYRIIVKNQKGCTDTTQ
                     VTVFNYGPKYAQVKQLIAGTGGIGGYCGPCHLNGAINGGKNFDTDASIVASWDRIKAR
                     AVDGLPTFMPEAPNSPLTLADRQKITDWVNAGHRQSD"
     gene            1143..>2830
                     /locus_tag="PLU11_14620"
     CDS             1143..>2830
                     /locus_tag="PLU11_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN60418.1"
                     /translation="MKQKLVAFIWAFFALQVLSAQENLVVTKEHNRVIIPVKVKLTGK
                     ITDSKTGEALAGAYIYFTDDKIGTSSDENGLYVLTNIPPGHHVVEVSFTGYSTLVEHI
                     EINADMQKDFALSTRIVENQGVVITGVSGATSIRKAPVPITSIKKATLLQTPSGNIID
                     ALSHVPGISQVSTGPAISKPIIRGLGANRVVTVNDGIRQEGQQWGDEHGIEIDEMSIS
                     KVEILKGPASLMYGSDALAGVVNFITNVPAPEGTLRANWLSNYQSNNGLLANNISVAG
                     NKNGFNWNIYGSHKSAGDYKNKHDGRVLNSRFNEKNLGGYLGINKGWGYSHLIFSRFD
                     QRLGLVEGDRDDANGKFILYAGTPIERIATEEDLESRKLFIPNQRVQHYKLVSDNNFA
                     IGQSRLKLNLGYQNNRRKEFGNAEDPEEQELFFDLKTINYNLQWQLPEMKEWHTSIGV
                     NGMQQQNKNKGAEVLIPEYSLFDFGAFVYFQRFYKDGTLSGGLRFDNRSVDGKEFFEG
                     TEEKFKAFTRNFSNISGSLGVSYEPAERVTVKANIARGFRAPTLAELASNGTHEG"
BASE COUNT          788 a          594 c          695 g          753 t
ORIGIN      
        1 catcgatacg ctgggccgtg tgatcaaaat tctcgccgac cgggaatata ccgcaggtac
       61 ctatacgctc gactttaaca gtgaaggatt acctaccggt atctactatg cgcgattcca
      121 gaatggagtg acacagcagg tgagggcgat gatgaaagtc agatagaaag ggtgtcgggt
      181 gtcaggtgtc gggtgtcggg tgttagctgt tagctgttag gtgttgggag ccatcttaga
      241 gcattatcat atttaccact gaatctcatt tacgaaaatt gctcataatc gtaaccataa
      301 gctcacagtt tgtattttgc ttcttgattt ttctttggct tcttggcatc ttggtatctt
      361 ggttcttatt tcggcgtctt ggtatcttgg ttctttttct tttttaatcg ctttgcctgt
      421 gccctgcatt cacccaatca gtgatcttct gccggtcagc taatgttaat ggactattcg
      481 gagcttccgg cataaacgtt ggcagtccgt ctacggcccg ggccttgatc cggtcccagc
      541 tggcgacaat gctggcatcg gtatcaaaat tctttccacc gtttatggcc ccattgaggt
      601 gacagggacc gcaataacca ccgataccgc ccgtgccggc aatcagttgt tttacctggg
      661 catatttggg accatagtta aagacggtta cctgcgtggt atcggtacag cccttttgat
      721 ttttaacaat gatgcgataa tcgccaggtg ccaggttgga gaaaataggg gaggattgga
      781 aagcggcact atttagctgg tagctgatcg tgtcacctac cggtgaagta attgtaaggg
      841 aaccattgtt cgaactaccc acggattccg ttttaaagta ttggatatca taactcactc
      901 ccaggcaggg gttgggtaag gcatctttct tattacaggc ctggaaatac attcctccag
      961 acaggaaaag caccgttatc gccattttct tccgcatata atcgatttga cagtcagtaa
     1021 attaaggatt cttgggctga tttggtattc agactaagta aatcagctca ggattagccg
     1081 gtttaaaaat ttatttgtat gaaacgttgt tgcaatttat atttgcaaca tagttacaaa
     1141 atatgaaaca aaaactggtc gcctttatct gggctttctt cgccctgcaa gtcctctctg
     1201 cacaggaaaa cctggttgta acaaaagagc ataatcgcgt aataataccg gtaaaagtaa
     1261 aactgaccgg gaagattacc gatagcaaga ccggtgaggc attggccggc gcctatattt
     1321 attttaccga tgataaaatc ggaacaagca gtgacgaaaa tgggttgtat gtgctgacga
     1381 atattccccc gggacaccat gtagtggaag tatcctttac cggttatagt acactggtag
     1441 agcatattga gatcaatgcc gatatgcaga aggattttgc cctttccacc cgtattgtgg
     1501 agaatcaggg ggtggtgatc accggcgttt ccggggccac cagtatccga aaagcacctg
     1561 tgcccattac ctccatcaaa aaagcaacct tattacaaac cccttccggg aatatcattg
     1621 atgcattaag tcatgtaccg ggaatatcgc aggtgtcaac cggaccggcc atttccaaac
     1681 cgattatccg gggactgggt gccaatcgcg tggtaacagt aaatgacggt atccgccagg
     1741 aaggacagca atggggagat gagcacggga tagagattga tgaaatgagt atttccaaag
     1801 tggaaatatt gaaaggaccg gcatccctga tgtatggcag tgatgcattg gcaggtgtgg
     1861 tgaattttat taccaatgta ccagcccctg aaggcaccct gcgggccaac tggctcagta
     1921 attatcaatc caataacgga ttactggcca ataatatcag tgtggcggga aataaaaacg
     1981 gatttaactg gaatatctat ggctcccata aatcagccgg ggattataaa aacaaacatg
     2041 atggccgggt gctgaattca cggtttaatg agaaaaatct gggtggttat ttaggtatca
     2101 ataagggttg gggctacagc cacttaatct ttagccgatt tgatcaacgt ctcggactgg
     2161 tggaaggtga ccgggatgac gccaacggaa aatttatcct ttatgccgga acaccgatcg
     2221 aaagaatcgc tacggaagaa gacctggagt cccgtaaact ctttataccg aatcaacggg
     2281 tgcagcacta taaactggtc agcgataata attttgctat tggccaaagc cggcttaaac
     2341 tcaacctggg ttaccagaat aatcgccgta aagaatttgg taatgcggaa gatcctgaag
     2401 aacaggaatt gttttttgat ctcaaaacga tcaactacaa cctgcaatgg caattgcctg
     2461 aaatgaaaga atggcatacc agtattgggg tgaatggaat gcaacagcaa aacaaaaata
     2521 aaggagctga agtgctgatc ccggaatata gtttatttga tttcggcgcc tttgtatact
     2581 tccagcggtt ttataaagat ggtaccctca gtgggggact tcggttcgat aacaggtcag
     2641 ttgatgggaa agaatttttc gaaggcactg aagaaaaatt caaagccttt acccgtaact
     2701 tctccaatat atccggaagt ctcggggtga gttatgaacc ggccgagcgg gttaccgtaa
     2761 aagcaaatat cgcccgtggc ttccgggcac ctactttagc ggaactggcc agtaacggaa
     2821 cacacgaggg
//
LOCUS       DAOPMW010000429         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_43818_length_2819_cov_5.278220, whole genome shotgun sequence.
ACCESSION   DAOPMW010000429 DAOPMW010000000
VERSION     DAOPMW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43818_length_2819_cov_5.278220"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2749)
                     /locus_tag="PLU11_14625"
     CDS             complement(<1..2749)
                     /locus_tag="PLU11_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPN60419.1"
                     /translation="MKKMLCLSLTLLFFGLAAIAQKKTITGKVTNQLTGEPLQGVSIL
                     VDKQKGGTTTKADGTYSVVVNSGGATLIFSYVGFTPQTVATEGKTVVDIAMTPVVTDN
                     NEVVVIGYGTQKKSHLTGAVSKYKNEKIDETPNSRLDQALQGKIAGVQVQNISSEAGS
                     DPKIRVRGISSINAGANPLVVVDGHPVPDGLAFVNMADVESVEVLKDAASSAIYGSRG
                     ASGVIIITTKSGKADKPKYSVKFSTGAKTQVELYPMMSTSEYTNLLFYEAALKSKDPS
                     ITPPSGSGIIANNERAAYVVENTIMGGFGANWQQEALRNANVRNLQISVSGGSRQAKY
                     FLSGGYQRDQGMMHHSEYDRFNLRSRLDLQLAKKVKLSFNLNPSYIKRERPSVNFIDF
                     VRFQSFLPVYHTEASAAFVSQVAQWANIKPGDFAQARHFNGRVYTGLMPDGSQWVTTS
                     TTDPFNTANNSPKSVMETRTITSDDYRVLSSGDLTYNILPGLDFKALGSVYMNYTQGL
                     DFTKRNSNREGDVNRGVYSNRLFVDLLSENTLNFSKLIKEHSVNVLAGFTAQKTTIKD
                     EQITGFDYPSDNITTLNTALQIDQAKTATFNLRNQIGLLSYLGRVNYTYKNRYLLSAS
                     YRADGSSYFAPGNKWGSFPSVSLGWVATEEKFTEKLDWLSNLKLRASYGVTGNNRIVD
                     FAFVDLLYAANYPLGTGTGTSVLGQVPSRDILSNTDITWERTFQYNGGIDISLFRNAI
                     TLSVDVYQSKTDQLLLRQAAMGFTGVPLAWNNIGRLQNRGIEFELTTNNIKRKDFRWT
                     TSANIAHNRNKILELGEEAFLLNQGERTELYMNRVGDPLIQYFGYKTDGVWLSQADIT
                     AAQAAGLTSPLSNVFVPGGLKLVDVNGDKIINADDRVVTGSPYPDFNWGVTNNFTYKS
                     FD"
BASE COUNT          744 a          696 c          576 g          803 t
ORIGIN      
        1 gatcaaaaga cttataggtg aagttattgg tcacccccca gttaaaatcg ggatatggcg
       61 aaccggttac cacccgatca tccgcattga tgatcttatc gccatttaca tctaccagtt
      121 tcaagccacc tggtacaaat acattgctta gcggactggt cagaccagct gcctgagcag
      181 cggtaatatc ggcttgtgaa agccatacgc catccgtttt atatccaaaa tactggatca
      241 gcggatcgcc aacccggttc atatataatt cagtacgttc accctggttg agcaggaatg
      301 cctcttcacc caattcaagt attttgttcc ggttatgcgc aatattggct gaagtcgtcc
      361 acctgaaatc ttttctttta atgttattag tggttaactc aaactcgatt cccctgttct
      421 gtaaacggcc gatattattc caggccagcg gtaccccggt aaagcccatg gcagcctgcc
      481 gtaataataa ctgatccgtc tttgactggt agacatccac tgataatgta atggcatttc
      541 tgaaaagact gatatcaata cctccattat actggaacgt acgctcccac gtaatatcag
      601 tattcgacaa tatatctctc gaaggcactt gtccgagtac actggtacct gttcccgtac
      661 ccagcggata gttggccgca tacaaaagat ccacaaaggc aaaatcaaca atccggttgt
      721 taccggtaac accataactg gccctgagct tcaggttgct cagccaatcc agcttctccg
      781 taaatttctc ttctgtggcc acccatccca gcgatacaga agggaatgac ccccatttat
      841 tgccaggcgc gaaataagaa ctgccatctg cccggtaact ggccgataat aaataccggt
      901 ttttataggt ataatttaca cgtcccaggt atgataacaa accaatctgg tttctcagat
      961 tgaatgttgc tgttttcgcc tgatcaatct ggagtgccgt atttaaagta gtaatattat
     1021 cactggggta atcgaagccg gtgatttgtt catctttaat ggtagttttc tgtgctgtaa
     1081 atccggccag aacgttcacc gaatgttcct taatcaattt tgaaaagttc aatgtgtttt
     1141 ctgagagcag atccacaaac agccggttgg aataaacacc ccggttcaca tctccttccc
     1201 ggttagagtt tcttttggta aaatcaagtc cctgtgtata attcatatat acactaccca
     1261 atgctttaaa atccaatccg ggtaaaatgt tatacgtcag gtcaccggaa ctcagtaccc
     1321 ggtaatcatc ggatgtgata gtccttgttt ccattactga ttttggtgaa ttattcgctg
     1381 tattaaacgg atcagtggta gaagtagtca cccactggct tccatccggc atcaacccgg
     1441 tatatacccg gccattaaaa tgcctggctt gtgcaaaatc gcctggtttt atatttgccc
     1501 actgcgccac ctggctgaca aaagcggcac tagcttcggt atggtaaacg ggaaggaagg
     1561 attgaaaacg gacaaaatca ataaagttca cagaaggcct ttcccgttta atataagacg
     1621 gattcagatt aaaactcagc ttcacttttt tagcaagctg cagatccagc cgtgaacgga
     1681 gattgaaacg atcatattca ctatgatgca tcatcccctg gtcgcgctgg tatccgcctg
     1741 ataaaaagta ttttgcctga cgtgagccac ccgatacact gatctgaagg ttccgtacat
     1801 tggcattccg taaagcctcc tgttgccagt tagccccgaa cccgcccatg attgtattct
     1861 ccactacata ggcggctctt tcattgttgg cgatgatgcc ggatccggaa ggcggtgtta
     1921 tgctcggatc cttggatttc agtgctgctt cataaaaaag caggttggtg tattccgacg
     1981 tactcatcat cggatataat tccacctggg ttttggcacc ggtggaaaat ttaacggagt
     2041 acttgggttt atcggcctta ccacttttcg tggtaatgat aataacccca ctggcacctc
     2101 ttgaaccata aatagcggag gaggctgcat ctttcaacac ttccactgat tccacatccg
     2161 ccatattcac aaaagccagt ccatccggca cagggtgtcc gtccacaacg accaatggat
     2221 tggccccggc attaatggag ctgattcccc tgaccctgat cttcggatca gatccggctt
     2281 cagaagaaat attctgtacc tgcacacctg caatcttgcc ctgcaaggcc tggtcaagcc
     2341 tggagttcgg cgtttcatca attttctcat ttttatactt ggaaaccgct ccggtcaggt
     2401 gcgatttctt ctgggtgcca taaccgatca ccaccacttc attattatcg gttacaacgg
     2461 gtgtcatagc aatatccact accgtttttc cttcagtggc aactgtctgt ggagtaaatc
     2521 caacataaga aaaaataagc gtagcacctc cactattgac gactacacta taggttccat
     2581 ctgcttttgt agtcgttccc cccttttgtt tgtcaactag gatagaaact ccctgcaacg
     2641 gttccccggt cagctggttg gtaactttac cggtaatggt ctttttttgt gcaatggcgg
     2701 ccagtccgaa aaacagcagc gtcaaagaca agcaaagcat ttttttcata ttagcagttt
     2761 aaatatttac aaatcgttat tagtttatta aatgaagcag caatttttcc gccttatcg
//
LOCUS       DAOPMW010000430         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_44027_length_2811_cov_3.567489, whole genome shotgun sequence.
ACCESSION   DAOPMW010000430 DAOPMW010000000
VERSION     DAOPMW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44027_length_2811_cov_3.567489"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLU11_14630"
     CDS             <1..339
                     /locus_tag="PLU11_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN60420.1"
                     /translation="DISGYVLKNVGKQELVKALEKIASGGIYFSEEVISELQRSSERK
                     KQIEDAHLTDREIEIVRLIEKEYNNKQIAEELFISERTVETHRKNIFRKTNTNSVIGL
                     VKYAYEHKLI"
     gene            767..1876
                     /locus_tag="PLU11_14635"
     CDS             767..1876
                     /locus_tag="PLU11_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPN60421.1"
                     /translation="MKPVLSILLFTLLLNYSQAQLSLTMLPSGGNKKATVSEQIGLTQ
                     VSIHYDRPGVKGRDGKVWGDLVHVGFTDPEFGTSKSSPWRAGANENTTIEFSTDVMIE
                     GQPLPAGKYGFFIAYDPTECTLIFSKNSGSWGHYFYSEKEDALRIKVKSQATDHSVEW
                     LKYEFNQQTENSAVLSLQWEKRAIPFKIEVDYYKTQLESFRQELRTEKGFFWLSWNQA
                     ADWCLQRNTNLEEALLWADSATSAGFSGDRIFGTWATKARILQKLGRTDESAAVMKKA
                     LPLGDMIDLHQYGRQLLNLKMNKEAFDVFKLNFDKNPNQFTTSMGLARGYSALGDFKN
                     ALKYINQALPLAPDEQNKKNVQALIDKLKEGKDIN"
     gene            1990..2457
                     /locus_tag="PLU11_14640"
     CDS             1990..2457
                     /locus_tag="PLU11_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60422.1"
                     /translation="MKRLLPLFLMLSFNTVLFSQTTEQRETRLLESIGTLSSAYIYNA
                     YIVIGSISDASVDGVYTDSLATAVLKEQQGMLDVVIESLSKLVKEKALKDINDVTYVN
                     TLTPLLKGLKAQAQSYIDFLKTKNTAHTTEFVRLRKSNWKSICGILNIPYKEN"
     gene            complement(2454..>2811)
                     /locus_tag="PLU11_14645"
     CDS             complement(2454..>2811)
                     /locus_tag="PLU11_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN60423.1"
                     /translation="RGKYVLVDFWASWCGPCREENPNVVENYKKFNPKNFTVLGVSLD
                     RPGQKARWVEAIHEDNLTWTHVSDLQFWNNAVARMYRVQSIPQNYLLDPEGKIIAKNL
                     RGPALEAKLCELLGCN"
BASE COUNT          854 a          614 c          632 g          711 t
ORIGIN      
        1 gatatatccg gttatgtatt aaagaatgtg gggaaacagg aactggtaaa ggcccttgaa
       61 aaaattgcca gtggcggcat ctacttcagc gaagaagtga tcagtgaatt gcagcgaagc
      121 agtgaacgga aaaaacaaat cgaagatgcg cacctgacag accgtgaaat tgaaattgtc
      181 cggctgatcg aaaaggaata caacaataaa cagatcgccg aggaattgtt catctctgaa
      241 cggaccgtgg aaacacaccg gaaaaacatt ttccgcaaga caaacaccaa cagtgtgatc
      301 gggctggtga aatatgcata tgagcataaa ctgatctgac ctgaacaatg aacccaaaac
      361 tggcatacat taatcaggga agaggcggcc atgttgttta caccgacacg atatcttcta
      421 tccggtttga ttttgaattt ggtggcggag actgcgttgc tataattttt gttccttcca
      481 tttccagttg ggaagccggg acccggcggc ccctttccga aagagatgaa atccttttat
      541 ttgttgcccg acagtccctg catgatcagg tgtcgaacgg gtattataaa ataatggata
      601 gctgtattga attattcaaa tcctgagccg gatgacaatt ccaggagtgt aaattgcctt
      661 cccgggcaga aagaatacac catttaacca tctttacttc cattcatcac gacggtactt
      721 tttatatgca agggattatt tagtttacca ggataaacca gccatcatga aacctgtatt
      781 atctattctg ttatttaccc ttttactaaa ctatagtcag gcacaactct ccttaaccat
      841 gctgccaagc ggtggtaata agaaagccac cgttagtgag caaattggcc tcacccaggt
      901 ctccattcat tatgaccgtc cgggggtgaa aggccgggac ggtaaggtat ggggagacct
      961 ggtccatgtg ggatttacag atccggagtt tggaaccagt aaatcctcgc cctggagagc
     1021 cggcgccaat gaaaacacga cgattgaatt cagcactgat gttatgattg aaggacaacc
     1081 cctgcccgca ggtaagtacg gtttttttat tgcctacgac ccaacagaat gcaccctgat
     1141 cttttcaaaa aattcaggtt cctggggaca ttatttctac agtgaaaaag aggatgccct
     1201 ccggataaaa gtaaaatcac aggcaaccga tcattcagtg gaatggctga aatatgagtt
     1261 caatcagcaa accgaaaaca gtgcggtgct ttcgcttcag tgggaaaaac gagctatccc
     1321 ttttaaaatt gaagtggact attacaaaac ccaactcgaa tcatttcgcc aggaacttag
     1381 aacggaaaaa gggtttttct ggctgagctg gaatcaggca gctgactggt gtctgcaacg
     1441 gaatacgaac ctggaagaag cattgctctg ggcggattct gccaccagcg ccggattcag
     1501 cggcgaccgg atatttggca cctgggcaac caaagcccgg atacttcaaa aactgggaag
     1561 aactgatgaa tccgctgctg tcatgaagaa agctctgccg ctcggagata tgattgatct
     1621 gcatcagtat ggacggcaac tgcttaacct gaaaatgaat aaagaagcct tcgatgtctt
     1681 taaactgaat tttgataaaa atcccaacca gttcacgacc tcaatgggac tggcacgggg
     1741 ctattctgca ctgggtgatt tcaaaaacgc acttaaatat atcaaccagg cattgccact
     1801 ggcgccggat gaacaaaata agaagaatgt ccaggcgctg attgataaac tgaaagaagg
     1861 aaaagatatt aactgatgat ttaaaaaaat cggttttaaa aaaagggcgg gtactgccct
     1921 ttttttatac cctgaagcgg gtattgaatt tgcctgctcc tgattgtaat ttcaggaaaa
     1981 gcacctatta tgaaacgcct tctcccctta tttttaatgc tgtctttcaa tactgttctt
     2041 ttttcacaaa ctactgaaca aagagaaacc agattacttg aaagcattgg aacattatcc
     2101 tccgcttata tctacaatgc gtatatagtc atcggaagca tttctgatgc gtctgtggat
     2161 ggggtatata ccgattccct ggccactgcc gtgttaaagg aacagcaagg aatgctggat
     2221 gttgtaatag agagcctgag caagctggta aaagaaaaag cgctgaaaga tattaacgat
     2281 gtaacatatg tcaataccct aacccccttg ctcaagggac taaaagcaca ggctcagtcc
     2341 tatattgatt ttttgaaaac aaaaaacacg gcacacacaa cggaattcgt aaggctcagg
     2401 aaatcgaact ggaaatcaat ctgcggaatt ctgaatattc catataagga aaattaatta
     2461 cagccaagta attcacaaag cttggcctcc agggcggggc cccgtaagtt tttagctata
     2521 atttttccct cagggtccag caggtaattt tggggtatac tctgaacgcg gtacatccgg
     2581 gccactgcat tattccagaa ttggagatca ctcacatgag tccaggtgag attatcttca
     2641 tgtatggctt ccacccaacg ggccttctgc ccgggacgat cgagcgaaac cccaagtacg
     2701 gtaaaatttt tggggttgaa ttttttataa ttctccacca cattgggatt ttcctcgcgg
     2761 caggggccgc accagcttgc ccagaaatcc accagcacat atttccctct g
//
LOCUS       DAOPMW010000431         2777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_44807_length_2777_cov_5.917708, whole genome shotgun sequence.
ACCESSION   DAOPMW010000431 DAOPMW010000000
VERSION     DAOPMW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44807_length_2777_cov_5.917708"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLU11_14650"
     CDS             <1..482
                     /locus_tag="PLU11_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPN60424.1"
                     /translation="RVDGLEERVAGGNFIDKTGKILGQHKGYPFYTIGQRKGLDIALG
                     RPVFVTSIDPEKNTVVLGDEADLEQNEMKVGKLNMIKYDQLTPGMEAVTKIRYKDSGA
                     LSNLYPENGTMNVRFYENVKGIAPGQSAVFYEGEDVIGGGIIQSGVRSRELGVNSPS"
     gene            731..1519
                     /locus_tag="PLU11_14655"
     CDS             731..1519
                     /locus_tag="PLU11_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60425.1"
                     /translation="MNKEKPPMRIFYSVRLCLLAFVSIAFFSCNEKEEFTTAPLADYL
                     PLTAKKYITYRLDSTVFTNFGRSTEIHKYQVKHLIDTMIMDNAGRPSYRVYRYLNDSA
                     ATQVWQPAGSYFITPLADQVEVVEDNLRFIKMHLPIRNGFSWKGNKFLPADPYGPLFS
                     FSNDDSMEDWDFYYDGDAGSFSYDGQNYADVYTVEQVDESFNVPITTPTAYAAKSRSV
                     DRYSKGIGLIYREYELWEYQPNPGGAGGPYKTGFGITMWMVDHN"
     gene            1565..>2777
                     /locus_tag="PLU11_14660"
     CDS             1565..>2777
                     /locus_tag="PLU11_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN60426.1"
                     /translation="MKKLLLLGFVCVFLGSEAGAQFTRYIVRLKDKGNASFTLANPGT
                     YLSQRAIDRRTKYSIPLDSNDLPVSPTYVTQIRNVANVTVLNVSKWLNAVSIQTSDPA
                     AITTISALPFVQSVGGLASRPGEGGRLITPNKFALEEVTTPLPPNTGKMGDINGDFFN
                     YGTSSYNEIHLHNGEFLHNIGLRGQGMQIAVLDGGFQNYTSLKAFDSINANGQVLSTW
                     DFVARNASVSEDHQHGMQCLSTIAANIPGQFVGKAPKASFHLFRTEDVASEYPIEEHN
                     WVCGAERADSAGVDIISSSLGYYDFDNAVFNYTYANMDGNTTMAAKGADLAVKKGLLV
                     FNSVGNEGGNAWNFLVTPSDGDSVIAVGAVSTTGAVGSFSSYGPSGDGQVKPDMASVG
                     VAALVQTTSNTI"
BASE COUNT          729 a          646 c          641 g          761 t
ORIGIN      
        1 gccgggtgga cggattggaa gaaagagtgg cgggtggaaa tttcattgac aaaactggta
       61 aaatattagg tcagcataaa ggttatcctt tctatactat cggtcaacgt aagggattgg
      121 atattgccct gggtcgtcct gtttttgtaa ccagcatcga tccggaaaaa aacaccgtag
      181 tgctgggtga tgaagccgat ctggaacaaa atgaaatgaa agtcggtaaa ctgaacatga
      241 tcaaatacga tcagctcaca ccgggcatgg aagccgttac caagatccgc tacaaagaca
      301 gtggcgcctt atccaacctt tacccggaaa acggcaccat gaatgtccgt ttttatgaaa
      361 atgtcaaggg catagcaccc ggtcaaagcg ccgtttttta tgagggcgag gatgtgatcg
      421 gcggagggat aattcagtcg ggagttagga gtcgggagtt aggagtcaac agcccctcct
      481 gagaattatt gtctctattt tgtttttatt gattttcaat catttaaatc atttacacac
      541 tcaatttacg gcctctgact cccgactgcc gactcccgac tgccaactgc ctactgccga
      601 ctgcctactg ccgactgccg tctaccgact gataatcagg gcaataaaca ccctaaattc
      661 ccgttattac atgcggagct gctatgtatc cccgggataa gtttgtagct ttatgtttta
      721 atttatatcc gtgaacaaag aaaaacctcc tatgcgcatt ttctactctg ttcgtctttg
      781 tctgctggcc tttgtcagca ttgccttttt ttcctgcaat gaaaaagaag aatttaccac
      841 tgcaccactt gcagattacc tgcctttgac tgctaagaag tatattactt accggctgga
      901 ctccaccgta tttaccaatt ttgggcggag tacagagatt cacaaatacc aggtaaaaca
      961 cctgatcgat accatgatca tggataatgc gggtcgtccc agctaccggg tctatcgtta
     1021 tctcaatgat tctgccgcta cacaggtttg gcaacctgct ggttcttatt ttatcacgcc
     1081 tcttgctgac caggtagaag tggtggaaga caatctccgt tttataaaaa tgcacctgcc
     1141 gatacgcaat gggttcagtt ggaaaggcaa caaattcctt cctgctgatc cttacgggcc
     1201 tcttttcagt ttcagtaatg atgatagtat ggaagactgg gatttctatt atgatggcga
     1261 tgcgggatca ttttcatatg atggtcagaa ctatgcggat gtttacaccg tagaacaggt
     1321 ggatgaatct ttcaatgttc ctattaccac acccactgct tacgccgcca aatcaagatc
     1381 cgttgaccgt tattcaaaag gaatcggtct gatttaccgc gaatatgaat tatgggaata
     1441 ccagcccaac ccgggcggtg ccggcggacc ttataaaaca ggttttggca ttacgatgtg
     1501 gatggttgat cataattaat ctaccggtta tccttacttt tgatcgggca aaagccggta
     1561 taccatgaaa aaattattac tcctgggatt tgtttgtgta tttctcggat ctgaagccgg
     1621 tgcccagttc acccgttata ttgtgcgact aaaagacaag gggaatgcct cctttacact
     1681 cgccaacccg ggaacctatc tttcccagcg ggccattgac cgccggacca aatacagtat
     1741 tccccttgac agtaacgacc tacccgtttc acccacttat gtaacccaga tcagaaatgt
     1801 ggccaacgtt acggtgctga atgtttctaa atggcttaat gcggtttcca ttcagacttc
     1861 tgatcccgct gctatcacta ccatcagcgc tcttcccttt gtacaaagtg tgggcggact
     1921 tgcctccaga cccggagaag gtggccgttt aatcactcca aacaaatttg cacttgaaga
     1981 agttaccacg cccctccctc ccaataccgg aaagatgggg gatatcaatg gagatttttt
     2041 caactacggc acttcctcct ataatgaaat acacctgcat aacggtgagt tcctgcataa
     2101 tatcggtttg cgcggacaag gtatgcagat cgcagtgctg gatggtggat tccagaacta
     2161 tacttcatta aaagcttttg acagtatcaa tgccaatgga caggtattga gtacctggga
     2221 ttttgttgcc cgaaatgcca gtgtatccga agaccaccag cacgggatgc aatgtctctc
     2281 aactattgcg gcaaacatac cgggccagtt tgttgggaaa gcccccaagg ccagcttcca
     2341 tcttttccgg acagaagatg tggcttcgga atacccgata gaggaacata actgggtttg
     2401 cggggccgaa cgggctgaca gtgcaggcgt ggatattatt tcctcttcgc ttggctatta
     2461 cgattttgat aacgcggttt ttaattatac ctacgctaat atggatggca ataccaccat
     2521 ggccgctaaa ggggcagacc ttgctgttaa aaaaggatta ctggtattta attcagtggg
     2581 aaatgaagga ggcaatgcct ggaatttcct ggttacccca tctgacggag atagtgtgat
     2641 tgctgttggt gcagtatcaa caacaggtgc tgtcggtagc ttctccagtt atggcccatc
     2701 cggcgatggt caggttaaac cggatatggc ttccgtaggt gtggctgcgt tagtacaaac
     2761 caccagcaat accattg
//
LOCUS       DAOPMW010000432         2776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_44832_length_2776_cov_4.845645, whole genome shotgun sequence.
ACCESSION   DAOPMW010000432 DAOPMW010000000
VERSION     DAOPMW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44832_length_2776_cov_4.845645"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLU11_14665"
     CDS             complement(<1..931)
                     /locus_tag="PLU11_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPN60427.1"
                     /translation="MLLRHLPFLRAVFFHSVSAFGGPQAHMGMMMKTFVEQTPYVTEK
                     ELMEYNAFCQLLPGASSTQTLTLIGYKRGGVPLAVLTLIIWILPASILMGALSFLLQY
                     IDKRQLGTDIFKFIPPMAAGFLGYAVLHIFPHAIKNTITWIIMICSVAATYFLFGKTW
                     VIPALIVAGGIATNFSQTRIRQVDEKPRKIRWWNFWLFGIIFIAAGILSETARKNDWY
                     NRKPINLFENTYRMGSLVFGGGQVLMPMMYEQWSVRPEAIKEKNPNAVQIDQEKLYTG
                     MGIVQAVPGPVFSIASFTGGMALKDMGTEMQVLG"
     gene            1030..2355
                     /locus_tag="PLU11_14670"
     CDS             1030..2355
                     /locus_tag="PLU11_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN60428.1"
                     /translation="MKQKLLTLLLFSPFILPAQKGTTDLTAKINAAADKIEAKTITWR
                     RDLHEHPELGNNEVRTAKIIADHLRSLGIEVIEGVGKTGVVGVLKGAKPGPCIGLRAD
                     MDGLPVVERVNLPFASKVKSTYNGQAVGVMHACGHDTHVSILMGVAEILAGMKKDLAG
                     TIKFVFQPAEEGPPEGEEGGAPLMIKDGVLENPQVDVMFGLHINSQTEVGKIKYRTGG
                     IMAASDWFKIKVKGKQTHGSQPWAGIDPVVVSAQIINGLQTIVSRQTELTKNPAVISV
                     SVINGGVRSNIIPEDVTMLGTIRTLDTGMQRIIHEKIALTAAKIAESSGASAETTIDK
                     KTLVTYNDPALTKKMIPSFIKAAGEENVIETEAVTGAEDFSFFAEKVPSIFFFIGGME
                     KGKDPKTAFPHHTPDFVLDESGMKTGVKAFCHLVFDYLQSAATPAKKAF"
     gene            2355..>2776
                     /locus_tag="PLU11_14675"
     CDS             2355..>2776
                     /locus_tag="PLU11_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN60429.1"
                     /translation="MIKLFILSGTFFLCAAFSIQAQRTDKKLQTELRSMVSGYHGDIG
                     IYVKNLRTGKTAVINADTVFPTASIVKVPILLGIADRMNKGELAYDSSLTYRDSLLYE
                     GSDILGSFKSGEKIALKKVLMLMLTTSDNTASLWLQSLA"
BASE COUNT          793 a          667 c          661 g          655 t
ORIGIN      
        1 agcccagcac ctgcatttcg gtgcccatat ccttcagggc catacccccg gtgaaggagg
       61 caattgaaaa gacgggcccg ggtacggcct gtacaatccc catcccggta tataattttt
      121 cctggtctat ctggaccgca ttcgggtttt tctcttttat cgcttccgga cgtacactcc
      181 attgttcata catcataggc atgagcacct gcccgccgcc aaatacgagg ctgcccatcc
      241 ggtaggtatt ttcaaataag ttgatcggtt ttcggttgta ccagtcattt ttacgggcgg
      301 tctccgaaag aatacccgcg gcaataaata tgatcccgaa taaccagaaa ttccaccaac
      361 ggatttttcg gggcttttca tccacctgac ggatacgggt ctgactgaaa ttggtggcaa
      421 tacccccggc cactatcaat gccggaatca cccaggtttt tccgaaaaga aaataagtag
      481 cggccacact gcagatcata atgatccacg tgatcgtgtt ttttatggca tgaggaaaaa
      541 tatgcagcac ggcatatcca aggaagccgg ccgccattgg tggaatgaat ttaaaaatat
      601 cggtgcccag ttgtctttta tcaatatact gtaagagaaa agaaagcgcc cccattaata
      661 tagaagctgg caggatccag ataatcagcg tgagtacggc taaaggaacc cctccccgtt
      721 tataaccgat cagggtcagc gtttgtgtag aagacgcccc gggtaataac tgacagaaag
      781 cgttgtattc catcagttcc ttttcagtaa cataaggcgt ttgctccaca aacgttttca
      841 tcatcatgcc catatgcgcc tgtggtccgc cgaaagcaga aacactgtgg aagaaaacag
      901 cccttaaaaa tggcaggtga cgtagcagca ttgaggttta aatataaaaa atttaaccca
      961 agcaattact gatcacacaa ttggcgcgga attatactaa attcattact taattaataa
     1021 taggtccaga tgaaacaaaa actgcttaca ctcctgctgt tttccccatt tatccttccg
     1081 gcacaaaagg gtacaaccga cctgacggca aaaatcaatg cggccgccga taaaattgaa
     1141 gccaaaacca ttacctggcg ccgtgacctg cacgaacacc cggagctggg caataatgaa
     1201 gtccggacgg caaaaatcat tgcggaccat ctccgctcac tgggcattga agtaattgaa
     1261 ggcgtgggaa aaacaggggt cgtgggtgtg ctcaaaggcg ccaaaccggg accctgtatc
     1321 ggtttaaggg ccgacatgga cggactaccg gtagtagaaa gggtaaacct gccctttgct
     1381 tccaaagtga agtcgactta taacggacaa gccgttggcg tgatgcatgc ctgtggtcac
     1441 gatacacatg tatctatatt aatgggggtg gccgaaatcc tcgccggtat gaagaaagac
     1501 cttgccggaa ccatcaaatt tgttttccaa cccgcggagg aaggtccgcc cgaaggggaa
     1561 gaaggcggcg ctccattaat gattaaagat ggcgtgctgg aaaacccaca ggtcgatgtg
     1621 atgtttggcc tgcatatcaa ctcccagacc gaggtcggca aaatcaaata ccggacaggt
     1681 ggcattatgg cggcttccga ttggttcaag ataaaagtaa agggaaaaca gacccatggt
     1741 tcacagccct gggccggtat agatcccgta gtggtgtcgg cgcaaatcat caacggtctt
     1801 cagacaattg tgagccggca gaccgaactg accaaaaacc cggcggtgat ttcggtcagt
     1861 gtgatcaatg gtggagtccg ttccaatatt atccccgaag atgtaaccat gctgggcacc
     1921 atccggacac tcgacacggg tatgcagcgg attattcatg aaaaaatcgc cctgacggct
     1981 gcaaaaattg ctgaaagttc cggcgccagc gccgaaacaa cgattgacaa aaaaaccctg
     2041 gtcacgtata atgatccggc attaaccaaa aaaatgatcc ccagttttat caaagcggcc
     2101 ggagaggaaa atgtaattga aacagaggcc gtgaccggtg cagaggattt ttccttcttt
     2161 gccgaaaaag tgccctccat attttttttc ataggtggaa tggaaaaagg aaaggatcct
     2221 aaaacggcgt ttccacatca cactcccgac ttcgtattgg atgaaagcgg gatgaaaacc
     2281 ggggtaaaag ccttttgcca cctcgtgttt gattacctgc agtcagccgc aactccagct
     2341 aaaaaagcat tctgatgata aagcttttca tattaagcgg tacatttttt ctgtgtgccg
     2401 ctttttctat ccaggcacaa cgcacggata aaaaattaca aaccgaactt cggtcgatgg
     2461 tcagtggtta ccatggcgat atcggtattt atgtcaaaaa cctgcgtacc gggaaaacag
     2521 ccgtgatcaa tgcggatacg gtttttccga ctgcgagtat tgtaaaagtg ccgatcctgc
     2581 tgggcattgc cgaccgcatg aataaaggag agcttgccta cgattcttct cttacttata
     2641 gagattccct cttgtatgaa ggttccgata tactgggttc attcaaatcc ggcgagaaga
     2701 tcgccctgaa aaaagtattg atgctgatgc tgaccaccag tgataatacc gccagtctct
     2761 ggctgcaaag cctggc
//
LOCUS       DAOPMW010000433         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_45545_length_2746_cov_3.831289, whole genome shotgun sequence.
ACCESSION   DAOPMW010000433 DAOPMW010000000
VERSION     DAOPMW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45545_length_2746_cov_3.831289"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLU11_14680"
     CDS             <1..847
                     /locus_tag="PLU11_14680"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPN60430.1"
                     /translation="FLLFCTYLYPMAVIKIPTNFNIDLEFEVPEFFRRILALFIDTIL
                     LIIYYKISVAFLTSIAGTMNSRSEENWMNLHWASLFIILGPIFFYHISMEILLNGQSL
                     GKKIMQLRVVNENGGKAGISQLFIRWMLRDIWFLLLLLMGLGKIYTGENTQAIILVLS
                     VIGFFITDIILVVASKKGQRLGDLLARTILIRTQTKGDIEDTVFIEVADSYVPSFPQI
                     MQLSDRDINSIKNILDTARRKGDYHMAETAAAKIKAHLKIESGMPAFDFLDLLLKDYN
                     FLSTN"
     gene            complement(861..1325)
                     /locus_tag="PLU11_14685"
     CDS             complement(861..1325)
                     /locus_tag="PLU11_14685"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPN60431.1"
                     /translation="MEQQQENSLFGLSIDSVTAGYLKETASWAKLLAIVGMIGCGFIV
                     IGGIVASMAISSATSELSREMGGSGASAAAGAAMMIFYIIMAIIYFFPCLFTLRFSNH
                     IKVALNANDQMALNEGMRNLKATFRFMGVITIIFISLFLLLFLLGGLGAVMG"
     gene            complement(1426..>2746)
                     /gene="proS"
                     /locus_tag="PLU11_14690"
     CDS             complement(1426..>2746)
                     /gene="proS"
                     /locus_tag="PLU11_14690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPN60432.1"
                     /translation="RMFKETGHVNAYFPLFVPKSLFEAEEKNAEGFAKECAVVTHYRL
                     KTDPDNKGKLIVDPEAKLEEELVVRPTSEAIIWNTYKDWIQSYRDLPILVNQWANVVR
                     WEMRTRLFLRTAEFLWQEGHTAHATKQEAIEEAEKMLHVYADFAENWMAVPVIKGVKS
                     ANERFAGAEDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFSDKENKLEYVWATS
                     WGVSTRLIGALVMAHSDDQGLILPPRIAPTQVVIVPIYKGDEQKAQIDARVQAIMSEL
                     KALGIRVKYDDNDNARPGWKFAEYEMKGVPVRLAIGARDLEKNVVEVARRDTKEKNVV
                     SLDGIAGYVNNLLTEIQQFMFDKAKAYRDANITPANSWEEFEKLLDEKGGFISAHWDG
                     TPETEEAIKEKTKATIRCIPLDNPQEEGECIFSGKPSVQRVLFARAY"
BASE COUNT          712 a          689 c          574 g          771 t
ORIGIN      
        1 atttctcctg ttctgtactt acctttaccc gatggctgta atcaagattc ccaccaattt
       61 caatattgac ctggaattcg aagtacctga attcttccgg aggatccttg ccctttttat
      121 tgataccatt ctcctgatca tttactacaa gatttcagta gcctttctta cgtcaatagc
      181 cgggactatg aacagccgat cggaagaaaa ctggatgaac ctccattggg cttccctttt
      241 tattatactg gggccgatct ttttttatca tatttcgatg gagatcctgc tgaacgggca
      301 gagcctggga aaaaagatca tgcaattgcg ggtggtgaat gagaacggcg gtaaagccgg
      361 tatcagccag ttatttatcc gctggatgct aagggatatc tggtttttgc tcttactctt
      421 aatgggttta ggtaaaatat ataccggaga aaacacacag gcgatcatac tggtactttc
      481 agtcattggc ttctttatca ccgacatcat actggttgta gcgagtaaaa aagggcagcg
      541 cttaggcgac ttgctggcga ggacgatcct tatccgcaca cagaccaaag gcgatataga
      601 agacaccgtg tttattgaag tagccgacag ctatgtcccc tcttttcctc agatcatgca
      661 gctaagcgac cgggatatca attccataaa aaacattctg gacaccgcgc gacgcaaagg
      721 ggattaccat atggcggaaa cagctgccgc aaaaataaag gcacacctga aaattgaatc
      781 aggtatgcct gcttttgatt ttttagattt actcctgaaa gactacaatt tcctttctac
      841 caattgaacc actggtctga tcagcccatt acggcaccaa gtcccccgag taagaataac
      901 agtaagaaca aagaaataaa gataatggtg atcaccccca tgaaacggaa ggtcgctttt
      961 aaattgcgca ttccttcatt cagggccatc tgatcatttg catttaatgc taccttgata
     1021 tggtttgaaa aacggagtgt aaaaagacag gggaaaaagt agataattgc cataatgata
     1081 taaaaaatca tcatcgccgc acctgctgca gctgaagcac cactgccgcc catttcacga
     1141 cttaactcac tggtggcact gctgatcgcc atagacgcca caatgcctcc gataacaata
     1201 aatccgcaac cgatcatccc tacaatagca agcagcttcg cccaggaagc ggtttctttt
     1261 aaataaccag ccgttacgct atcaatgcta aggccaaata atgagttttc ctgttgttgt
     1321 tccatacaat ttttttttag gtttcagtaa atataaaact ccggtctgtt tactgcaaat
     1381 aattttgcgc tgaacaaacc ggagcggttg aaatcgatcc acggcttaat aagcccttgc
     1441 aaaaagcacc cgttgtactg aaggtttccc agaaaagata cactcccctt cttcctgtgg
     1501 attatccaac ggaatacaac ggattgtagc tttggtcttt tccttaatgg cttcttcagt
     1561 ttcaggagtt ccatcccaat gggcggaaat aaaaccacct ttttcgtcca ggagtttttc
     1621 aaactcctcc cagctgtttg ccggtgtgat attggcatcg cggtaggctt ttgccttatc
     1681 aaacatgaat tgctgaatct ccgtcaacag attattgaca tacccggcaa tgccatcaag
     1741 gctgaccaca ttcttttctt tcgtatcccg gcgggccact tccaccacat ttttctcgag
     1801 gtcacgggca ccgatagcca gacgtaccgg tacacccttc atttcatatt ctgcaaactt
     1861 ccagccgggc cgggcattgt cattatcatc atatttgacc cggataccaa gggctttcag
     1921 ctcactcatg atcgcctgca ccctggcatc gatctgggct ttctgttcat ctcctttata
     1981 aatcggtacg atcaccacct gggtaggagc aatccggggt ggcaggatga gtccctgatc
     2041 atcactatgt gccattacca gggctccgat cagacgggta cttacgcccc aactggtggc
     2101 ccatacatac tccagtttgt tttccttatc actaaatttc acatcaaatg ccttggcaaa
     2161 attctgtccc agaaaatggg atgtaccggc ttgtaatgct ttaccgtcct gcatcaacgc
     2221 ttcaatgcaa tacgtatcct ccgctccggc aaagcgttca ttggcggatt tcacgccttt
     2281 gatcacagga acggccatcc agttttcggc aaaatcggca tagacatgaa gcattttctc
     2341 cgcttcttca atggcttcct gtttggtggc atgcgcggta tgtccttcct gccagagaaa
     2401 ctcagctgtg cgaaggaata agcgggttcg catttcccat cgtaccacat tggcccactg
     2461 gttcaccagg atgggcaggt cgcggtagga ttgtatccag tctttatatg tattccagat
     2521 gatggcctct gaagtcgggc gcaccaccag ttcttcctcc agcttggctt cgggatctac
     2581 aatgagttta cctttattat ccgggtcggt tttcagtcgg taatgagtca ccaccgcgca
     2641 ttctttggca aaaccttcgg catttttctc ttccgcctca aacagacttt ttggcacgaa
     2701 caaagggaaa taggcgttta cgtgtcctgt ttccttgaac attcgg
//
LOCUS       DAOPMW010000434         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_46691_length_2697_cov_6.156699, whole genome shotgun sequence.
ACCESSION   DAOPMW010000434 DAOPMW010000000
VERSION     DAOPMW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46691_length_2697_cov_6.156699"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..2227
                     /locus_tag="PLU11_14695"
     CDS             80..2227
                     /locus_tag="PLU11_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HPN60433.1"
                     /translation="MKKLLIASLLFLSFSTAFAENGYDLWLRYLKITHTGTLVQYKKQ
                     VSSPALAGSSPTLLAAKEELQKGLSGLLGYRIPVNNSITTKSTLVIGVAGTPSLRLTP
                     ANEKKLISLGSEGFLINSDKKDNGCILITANTDLGILYGVFHFLRLMQTQQDLRQLDI
                     VSIPRLKLRLLNHWDNLNRTVERGYAGFSIWDWHKLPGYVDKRYIDYARANASIGING
                     AVLNNVNANALILTSQYLQKVKILADVFRPYGIKVYLSAKFSSPIETGKLKTADPLNE
                     EVRQWWKDKAKEIYSLIPDFGGFLVKANSEGQPGPQDYKRTHADGANMLADAVAPYKG
                     NVIWRAFVYASENPVDRHKQAYDEFVPLDGQFRNNVMVQVKNGAIDFMPREPFHPLFG
                     AMPRTPLMPEFQITQEYTGQSTNLVYLAPMFKEVLESDTYSNGPGSTVAHIIDGSTNQ
                     DRLNGMAGVSNIGNDINWCGHPFAQANWYAYGRLCWDYRLSSAALAEEWLRSTFSNDL
                     QFITPARSMMLESRETMVNFSNPLGLHHIMGANGHYGPGPWVSFLNRPEWNSVYYHKA
                     DSIGVGFNRTQSGSNAIAQYFPEARKQWEDLKTIDEKYLLWFHHVPWTYPTKSGRSLW
                     NELCYRYYTGADQVKEMQQTWDRLKTFVDPERFSHVKQLLAIQQEEAIWWRNSCLLYF
                     QTFSKMPIPANYEQPDKTLEYYKSLRFPFAPGN"
     gene            2442..>2697
                     /locus_tag="PLU11_14700"
     CDS             2442..>2697
                     /locus_tag="PLU11_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPN60434.1"
                     /translation="MKSMLKNVIVLFFLNSMTAYGQQPLKNESLNYQQVNTYMNPVLP
                     GDHPDPTLIRIGKDFYHCGSSFHFNPYLPIYHSTDMVHWEI"
BASE COUNT          807 a          659 c          587 g          644 t
ORIGIN      
        1 aacaccattg ttgtccgttc ggatctgatt atccggagct cctccctgaa aaataaaaaa
       61 tgaaactcat ccgccagata tgaaaaagct attaattgcc tccctccttt ttctatcctt
      121 cagcacggcc tttgctgaaa acggatacga cttatggctc cgctacttaa aaatcaccca
      181 taccggaaca cttgtccaat acaaaaaaca ggtcagttcg cctgccttgg ccggcagttc
      241 tcctaccctg cttgctgcaa aagaagaatt acaaaaaggg cttagcggtt tgcttggcta
      301 tcgtataccg gtcaataatt ccattaccac taaatccacg ctggtaatcg gcgtagcggg
      361 aaccccatcc ctcaggctca ccccggcaaa tgaaaaaaaa ctgatcagcc tcggaagtga
      421 aggcttcctg ataaacagtg ataaaaaaga caatggctgt atcctgatca cagcgaatac
      481 ggatttaggg atcctctatg gggttttcca ttttctgcgc ctgatgcaaa cccaacagga
      541 tctccggcag cttgacatag tttccattcc ccgattaaaa ctccggctgc ttaatcattg
      601 ggataacctg aacagaacag tcgaaagagg gtatgcgggt ttcagtatct gggactggca
      661 taaactaccg ggttatgtag acaaacgata cattgattat gccagggcca atgcctccat
      721 tggtattaac ggagcggtat taaacaacgt aaatgccaat gcactcatac tcaccagcca
      781 atacctgcag aaagtaaaaa tattagccga tgtatttcgc ccctacggca tcaaggttta
      841 tctctctgct aaattcagtt cccccattga aacaggtaaa ttaaaaacag ccgatcccct
      901 gaatgaagaa gtcaggcaat ggtggaaaga caaagccaaa gaaatctatt cactgattcc
      961 tgactttggc ggattcctgg tcaaagcaaa cagtgaggga caacccggtc cgcaggatta
     1021 caaaagaacc catgcagacg gtgcgaatat gctggcagat gccgtagccc cctataaagg
     1081 aaatgtgatc tggcgggctt ttgtatacgc ctccgaaaac ccggtggacc gccataaaca
     1141 ggcctatgat gaatttgtgc cgcttgacgg acagttcagg aataacgtga tggtacaggt
     1201 gaaaaacggc gcgattgatt ttatgcccag ggaacccttt catccccttt ttggcgccat
     1261 gccccgcaca cccctgatgc ctgaatttca gattacccag gaatatacag gacaatccac
     1321 gaatcttgta tacctcgctc cgatgtttaa agaagtactg gaaagtgata cctatagtaa
     1381 cggacctggc agtaccgttg cccacatcat tgatggcagt acgaatcaag atcgcctgaa
     1441 cggaatggcc ggcgtatcca atatcggaaa tgatatcaac tggtgcggcc acccgtttgc
     1501 acaagccaac tggtatgcat atggaagatt atgctgggac tacagactga gcagcgcggc
     1561 cctcgcagaa gaatggttgc gcagtacttt ctccaatgat ttgcaattta taacaccggc
     1621 cagatcgatg atgctggaaa gccgggaaac gatggtgaac ttttctaacc cgctgggatt
     1681 acaccatatc atgggggcaa atggacatta cggacctggt ccctgggtca gcttcttaaa
     1741 caggccggaa tggaattccg tctattacca taaagcggat agtattggcg tcggatttaa
     1801 ccggacccaa agtggcagta acgcaattgc acaatatttc cccgaggcaa ggaaacaatg
     1861 ggaagacctt aaaacgatag acgaaaagta cctgttgtgg tttcaccatg tcccttggac
     1921 gtaccctacc aaaagcggcc gctccctctg gaatgaactc tgctaccgtt attacaccgg
     1981 ggccgaccag gtgaaagaga tgcagcaaac atgggaccgg ctaaaaactt ttgtagaccc
     2041 ggaacggttt tctcacgtga agcagctatt ggctattcaa caggaagaag caatctggtg
     2101 gcgtaattcc tgtttactgt acttccagac ctttagtaaa atgccgatac cggcaaacta
     2161 tgaacaacct gataaaactc ttgagtacta taaaagcctg cgtttcccat ttgcaccggg
     2221 caactgacgg gaatcaaggc tgaccttgtt ttcaataagc cggtaataat acaggcatta
     2281 atacagctga gcaaagacga aaacaggtta ttgtatcctg aaatattttt cgtggaaatg
     2341 aagccggaat aaagtgatca atgaccagta tcttctgaag acccgcaccc tgattttgca
     2401 atcggttaca accgggaaat taaaccatga atgatattgc catgaaatca atgctaaaaa
     2461 atgttatcgt cctgttcttt ttaaacagca tgaccgcgta tgggcaacag cccttgaaaa
     2521 acgaatccct caattaccaa caggtaaaca catacatgaa tccggtgtta ccgggcgatc
     2581 accccgaccc aaccttaata agaatcggga aggactttta ccattgcgga tcatcttttc
     2641 atttcaaccc atacctgccc atctatcatt caaccgacat ggtacactgg gaaatta
//
LOCUS       DAOPMW010000435         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_46946_length_2687_cov_6.405015, whole genome shotgun sequence.
ACCESSION   DAOPMW010000435 DAOPMW010000000
VERSION     DAOPMW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46946_length_2687_cov_6.405015"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLU11_14705"
     CDS             <1..273
                     /locus_tag="PLU11_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60435.1"
                     /translation="VIMNELSSLIFDADLKTDINLYRQNLQYAYISKLISMMKDPAYD
                     HASASASFENLKQLRAKMDKLNTGNEQTKAHRERLKFMIDKALVVK"
     gene            complement(794..1465)
                     /locus_tag="PLU11_14710"
     CDS             complement(794..1465)
                     /locus_tag="PLU11_14710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN60436.1"
                     /translation="MLLSCSTSERVPVNNPPPPPPPGGPLKTWLALGDSYTIGQGVDA
                     ADRFPTQTTNLLIQAGINMGTPDYIAATGWTSGVLTNQVQSQNPPQYDVVSLLIGVND
                     QYQTHDTTGYRLRFTGLLEKSIQQARGKKENVFVLSIPDYSVTPFAQYSDTTRIRQEI
                     DWFNAINRQVTAQFQCAYLDITPSTREALYDRTLLASDGLHPSKIEYRKWAVRLLPLV
                     QAVVR"
     gene            1607..>2687
                     /locus_tag="PLU11_14715"
     CDS             1607..>2687
                     /locus_tag="PLU11_14715"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPN60437.1"
                     /translation="MQIAVLGAGMVGRAIAIDLAKDFDVTSFDLNPANLLELHKRNKA
                     IKTLTVNLNQYEQFEHWLAPYDLVVTAVPGFIGYQTLEAVIKAGKNVVDISFFPEDAL
                     QLDKLAKEKGVTVITDCGVAPGMSNLILGRYNKEMEVTAFECYVGGLPKERKPPFEYK
                     APFSPVDVIQEYIRPARLVEKGKIVTKPALSEREIMYFEEAGALEAFNTDGLRSLVYT
                     MGHIPEMKEKTLRYPGHIDLIISLQQAGFFDTTPLRFNQTEISPLDFTSRLLLKEWKL
                     EPGEEEFTVMKVIVKGKNKSITWDLYNEYDPVTKTSSMARTTGYTCTAAVHLITDGLF
                     TQKGVFPPELIGDKKECFDAVTDYLK"
BASE COUNT          751 a          622 c          649 g          665 t
ORIGIN      
        1 gtgatcatga atgagctcag cagcctgatc tttgatgccg acctgaaaac ggatatcaac
       61 ctttaccggc agaacctgca atatgcctat atcagcaagc tgatcagtat gatgaaggac
      121 cccgcttatg accatgcctc agcttctgct tcctttgaaa acctgaaaca actgcgggca
      181 aaaatggata aactgaatac aggtaacgaa caaacaaaag cgcaccggga gagattgaag
      241 tttatgattg ataaggcgtt ggtagtaaag taaagtacga ggttagaagt acgaagtacg
      301 aaccccgaag tatcgggacg tacgggattt cgtaaaacga gagcgttgtt tttaaatggt
      361 gaggtagtgc tgtcatttcg agcgaagcga gaaatcttgt atttcccttt tctctcccct
      421 accttgccgt cattccgaac gaagtgaggg accttgtatt taaaaagtac gaagtacgaa
      481 gttagaagta cgaagtacga atcccgaagt gtcgggatat tcgggattcc gtaaaacgac
      541 gatcagtatg ttatgtaagg atcttttctt agcaggggct gttacctgac atccgacaac
      601 tgacatctga caacagacga accttgtact tgaacttcag caaaaaatac aaggtccctc
      661 gctgcgctcg aaatcggaga tacccgacat acgttttata accgatctgt ttctataata
      721 ataccagccc ttcaggctta aacaaaatgc tctcaggagg ggctgttgac tactaacttc
      781 taactacgaa ctactatcta accaccgctt gcaccaacgg caacaacctc actgcccact
      841 tcctgtactc aatcttcgac ggatgcaaac catccgaagc cagtaaggta cggtcataca
      901 atgcctccct ggtggagggc gtaatatcaa gataggcaca ttgaaactgc gctgttacct
      961 gcctgttaat tgcattgaac cagtcgatct cctgccggat acgggtcgta tcggaatatt
     1021 gggcaaaggg agtgacacta taatcgggaa tcgataacac aaatacattt tcctttttac
     1081 cccgggcctg ttgaattgat ttttccagca atcccgtaaa ccgcagccgg taacccgtag
     1141 tatcatgggt ctggtactga tcattcaccc cgatcagcag actcaccaca tcatattgtg
     1201 gagggttttg tgattgaacc tgattggtca gcaccccgct ggtccagccg gtggcagcga
     1261 tataatcggg ggtgcccata ttaatcccgg cttgtatcag gagattagtg gtctgggttg
     1321 gaaagcggtc ggcggcatcc actccctgtc cgatcgtata agaatcgccc agggccagcc
     1381 aggtttttaa cggtccaccg ggtgggggtg gcgggggcgg attgtttacc ggtacccgtt
     1441 ccgaagtgga acaggacagt agcaaacaaa aaatcatata aaggaaaaag aatcgcatta
     1501 gtcaatttac gtttgaatcg taaaaaaagt tcatcaggtg ggcagataac acaggtcata
     1561 agtgtatttt gcgctaattt ttatttttga tcaaagttta aacgttatgc agattgctgt
     1621 tttaggtgcg ggtatggtag gccgggctat tgcgatagac ctggcaaagg attttgacgt
     1681 caccagtttt gacctgaatc ctgccaatct gctggaactc cataaacgga ataaagccat
     1741 taagacgctt acggtcaatc tgaaccagta tgagcagttt gaacattggc tcgcgccgta
     1801 tgacctggtc gtcaccgccg tgcctggttt tattggttac cagaccctgg aagccgtcat
     1861 aaaagccggc aaaaacgtgg tcgatatttc ctttttcccg gaagatgccc tgcagctgga
     1921 taaactggcg aaagaaaaag gggtaacggt gattacggat tgtggcgtag cgcccggtat
     1981 gagcaacctg atcctcggtc gttacaacaa agaaatggaa gtcaccgcct ttgaatgtta
     2041 tgtaggtgga ctccctaaag aacgaaaacc gccctttgaa tataaagccc ctttctcccc
     2101 ggtggatgtg atccaggaat atatccgtcc ggcccggctc gtggaaaaag gaaagatcgt
     2161 caccaaaccc gctttgagcg aaagagagat catgtatttt gaagaggccg gtgcattgga
     2221 agcctttaat accgatggtt tgcgctcatt ggtgtacacc atgggacata ttccggaaat
     2281 gaaagaaaag acgctgcgtt acccgggaca tatcgacctg attatttccc tccagcaggc
     2341 cggttttttt gataccacgc ccttacgatt caatcagact gaaatttccc cgctggattt
     2401 cacctcccgc ctgctgttaa aggaatggaa actggaaccg ggcgaagaag agttcaccgt
     2461 aatgaaagtc attgtaaaag gaaaaaacaa atcgatcacc tgggatttat ataacgagta
     2521 cgatccggtc accaaaacct cttccatggc ccgcaccacc ggctacacct gtaccgccgc
     2581 ggtgcacctg attaccgatg gtctgtttac ccaaaaaggc gtctttcctc ccgaactgat
     2641 cggggataag aaggagtgtt ttgatgcggt gacggattat ctgaaga
//
LOCUS       DAOPMW010000436         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_47070_length_2683_cov_4.465753, whole genome shotgun sequence.
ACCESSION   DAOPMW010000436 DAOPMW010000000
VERSION     DAOPMW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47070_length_2683_cov_4.465753"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLU11_14720"
     CDS             <1..184
                     /locus_tag="PLU11_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60438.1"
                     /translation="ILVPFVNNSDSFANWVMAAVPFAAFHGCTYLYSGFRIVPILFFW
                     ATVAFIIGFQYWGPGW"
     gene            621..1292
                     /gene="purQ"
                     /locus_tag="PLU11_14725"
     CDS             621..1292
                     /gene="purQ"
                     /locus_tag="PLU11_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPN60439.1"
                     /translation="MKFGVVVFPGSNCDRDMYDALRYDLDQEVTMLWHKDRDLSSFTT
                     DDCIILPGGFSYGDYLRCGAIARFSPMMQSVIEFANAGGKVFGVCNGFQILCESQLLP
                     GGLLRNANQQFVCKNVYITNETGKPYMIPVAHGEGRYYADNQTLDALEANGQVIFYYC
                     DETGKIGGAANPNGSTRHIAGICNASRNVFGMMPHPERACSSALGNTDGREIIRTLLM
                     AEQLV"
     gene            1435..1887
                     /locus_tag="PLU11_14730"
     CDS             1435..1887
                     /locus_tag="PLU11_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HPN60440.1"
                     /translation="MKKWLLALVLMGFAGLAQAQLNPISWTFSAKKISENTYELQMKA
                     SIQTNWHLYSQTQPDDAVAMPTTFVLSPNPLFTLEGKIKEIGKMEKYTDKILKVSANQ
                     YSNTVTFTQVVKLKGKAKTSITGTVEYQTCDDQKCLPPKKVSFKVALG"
     gene            1960..>2683
                     /locus_tag="PLU11_14735"
     CDS             1960..>2683
                     /locus_tag="PLU11_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HPN60441.1"
                     /translation="MSIPRFFRLLLPVVFVFFTATVMAQEAAAYTWKVTSKKTAEREY
                     EISFSTAGKGGGQLYAPGQDLGGVASVALVFPDSGISVTQPIKSVSGAATSFASPLFE
                     NKEVTVFEGAVEWKTTIRFRDTVPGTLQGSLTYFYGSKTQADEFSPDPALLFNVPLEG
                     GMKADPLKVASIDINNPVVPCGDEGTENKSLITIFLLGLAGGLIALLTPCVFPMIPVT
                     VTFFTKKSTDRKKGVTNALLYGF"
BASE COUNT          699 a          624 c          650 g          710 t
ORIGIN      
        1 catcctggtg ccctttgtga ataatagcga cagttttgcc aactgggtca tggctgccgt
       61 gcccttcgcc gcttttcatg gctgtaccta cctctactcc ggcttccgga tcgtgcccat
      121 cctctttttc tgggccacag tggcttttat tattggtttt cagtactggg gaccgggctg
      181 gtaaggggaa gtggtaagta ttaagttata agttataagt aagaagggat tagttgtact
      241 atgaggcctt gtcatcctga cgaaggaagg ataaggcagc cgaccaagaa gacaacttca
      301 atcccgaccg cagggagggt tctggctgtt gactactaac taccgactac taactaccga
      361 ctattctgtc atcccgcttt gccctccgga gtgaaacgca ggagggaagc gaagcgaggg
      421 ataaggcagc cattttcctg cgatagcact gttgccggtc tgacttgact tttcagcctg
      481 aagcaaaaat caataaataa taattaataa taatcaacaa tcccgggcgt ttcgatggca
      541 gacagttgtt cacacctgct tttccagctt ctgccagacc ggcactttac ctatctttgc
      601 ccttcaaaat caccaccgat atgaaatttg gcgttgttgt atttccgggt tctaattgcg
      661 accgcgatat gtatgatgct ttacgttatg acctggatca ggaagtaacc atgctctggc
      721 ataaagaccg cgacctgagc agctttacaa ccgatgattg tattattctt cccggtggtt
      781 tttcctacgg cgattatctc cgttgcggcg cgatcgcccg ctttagtccc atgatgcagt
      841 ccgtgatcga atttgccaat gcaggaggaa aggttttcgg ggtttgtaat gggttccaga
      901 ttctctgcga atcacagctg ctgcccggcg gattactccg caatgccaat cagcagtttg
      961 tttgcaaaaa tgtctatatc accaacgaaa ccggtaaacc ctatatgatt cccgtagccc
     1021 atggcgaagg acggtattat gcggataacc agacgctcga tgcactggag gccaatggcc
     1081 aggtgatttt ctattattgc gatgaaaccg gtaaaattgg cggagccgct aatccaaacg
     1141 ggtctactcg tcatattgcg ggaatttgta atgcaagccg taatgtattt ggcatgatgc
     1201 cgcacccgga aagggcctgt agcagcgccc tggggaatac ggatggccgg gaaattatcc
     1261 gcaccctcct gatggcggag caactggtat aagggttttc tcatctgtat aaaggccatt
     1321 gtaccattag tcacagtggc ccccaattcg taaaattaga tttgcattgg ttttaacaga
     1381 accctgacat ataaagcagg atttttgcac gtaaataagc ataaacagga aagtatgaaa
     1441 aaatggttac tcgctttggt acttatgggc tttgctggtc tggcccaggc gcagctcaat
     1501 ccgatcagct ggaccttcag tgccaaaaag atttcagaaa atacgtacga actgcagatg
     1561 aaggccagca tacagaccaa ttggcacctg tattcacaga cacaacccga tgacgcggtg
     1621 gcgatgccca ctacttttgt cctgagtcca aaccctttat tcaccctcga aggaaagatt
     1681 aaggaaatcg gcaaaatgga aaagtatacc gataagattt taaaggtatc ggccaaccag
     1741 tattcaaata cggtcacttt cacccaggtg gtgaaattga aagggaaagc caagaccagc
     1801 atcacaggca cggtggaata ccagacctgt gatgatcaga aatgcctgcc tcccaaaaaa
     1861 gtaagtttta aagtagccct gggttaacca ggttattata cattatccgg cagggttgta
     1921 tggtttgtac atacagccct tttttataaa acagcaacca tgtccattcc ccgttttttt
     1981 cgtttactgc tccccgtagt ttttgttttt tttactgcta ctgtaatggc gcaggaggct
     2041 gccgcttata cctggaaagt gaccagtaaa aaaaccgctg aacgggaata cgagatcagt
     2101 ttcagtaccg ccggaaaggg tgggggacaa ttatatgcac ccggacagga tcttggtggg
     2161 gtggcttccg tagcacttgt ttttcctgac tccggtatca gcgttacaca gccaataaaa
     2221 tccgtatcgg gtgcggctac ttcatttgcc agccccttat ttgaaaataa agaagtcact
     2281 gtttttgaag gggcggtgga atggaaaacc accatccggt tccgggatac ggtacccggt
     2341 accttacagg gcagcctgac ttatttctat ggcagcaaaa cccaggccga tgaattcagt
     2401 cccgatccgg ccctgctctt taatgtgccg ctcgaaggcg ggatgaaagc agatccgctc
     2461 aaagtcgctt ctattgatat caataatccg gtagtccctt gtggggatga gggcacagag
     2521 aacaagagtt taatcacgat atttttattg ggtttagcag gcggattgat tgcactgctg
     2581 acgccttgcg tgttcccgat gattccggta acggttacat tctttacaaa gaaatcaacg
     2641 gaccggaaga aaggggtgac caatgcctta ctctatggtt ttt
//
LOCUS       DAOPMW010000437         2661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_47638_length_2661_cov_8.121259, whole genome shotgun sequence.
ACCESSION   DAOPMW010000437 DAOPMW010000000
VERSION     DAOPMW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47638_length_2661_cov_8.121259"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..537)
                     /locus_tag="PLU11_14740"
     CDS             complement(154..537)
                     /locus_tag="PLU11_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPN60442.1"
                     /translation="MENRVKKYDLEDRLVDFACICLEVCDLLPPTRAGQNLEYQLSKS
                     STAAALNYGEAQAAESTADFIHKMKVVLKEIRESRVNLKIICRKPVIVNEKTDSCLNE
                     ANELMAIFIKSINTAKKNLHPSKTK"
     gene            800..2080
                     /locus_tag="PLU11_14745"
     CDS             800..2080
                     /locus_tag="PLU11_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPN60443.1"
                     /translation="MNERLLQYIWQFQYFAAGELLTTTGEPVQVIQQGQYNTNQGPDF
                     SSAKIKIAGTTWVGSVELHIRSSDWDKHKHQHDRNYDNVILHVVWEEDGWNYPVPVLE
                     LKGRVPGLLLNRYALLMENPGFIPCEKNITGINELSWTSWKDRLLSERLERKSGLMQQ
                     FLEQNGNHWEEATWWMMARNFGIKVNTEAFESIARSLPVSLLSKQKHQLILLEALLMG
                     QAGLLGDEKPGDKYYTLLQREYRFQQTKYQLRPIAVPIHFLRMRPGNFPTVRLAQLAV
                     LLFQSESLFAKIKETRSLEELKQFFNITANDYWHYHYSFDEASPYKPKRLGSVMLDNL
                     IINTLCPLLFTYGSYHREQEYKDRAVQWLEQTTAESNTVTRGYSQLGVANKNAFDSQA
                     LIELKQSYCDQKRCLECAVGATLLRSQESVVGSR"
     gene            complement(2208..>2661)
                     /locus_tag="PLU11_14750"
     CDS             complement(2208..>2661)
                     /locus_tag="PLU11_14750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPN60444.1"
                     /translation="SLSYNVNLGQNIDMTSSVVYTGDLRTTCTHLQSQSAVETDAPVD
                     NNGKGERFSPTDLMATSLATCMVTVMGIKARTMGFDLNEVRIDVLKIMKADPRRVGGI
                     NLTFHIPDSLKEIDEKTKQILKNTGETCPVIKSIHPDIEVNIDWGSWS"
BASE COUNT          721 a          577 c          597 g          766 t
ORIGIN      
        1 tggtgatttt tataatgtga tggggttgcc ggtgagtaag gtggttaagg cgttggagag
       61 gtttggggat gagaaataag aaatgaaaaa taaggaataa ggaatgagaa agtggggttc
      121 ttagaaattt tggtggaatt ttcttttcat agtttatttg gtttttgaag ggtgaagatt
      181 ctttttggcg gtgttgatgc tttttataaa tatggccatt aattcattgg cctcattcag
      241 gcagctatcg gttttttcat ttactataac tggtttgcgg caaattattt tcaggttaac
      301 acggctttcc ctgatctctt tcagtaccac tttcatttta tgaataaagt ctgcagtgga
      361 ttcagcagcc tgtgcctccc cataattcag cgccgcggct gtgctgcttt ttgatagctg
      421 atactccaga ttctgacctg ctcttgtagg tggaagtaaa tcacaaacct ccagacatat
      481 acaagcaaag tcaaccagcc tgtcttcgag atcgtacttt tttactcgat tttccataga
      541 aaaaatttgc agctaattta gggatatccg gcaatcgaaa tttattcgtc tcctgtgcgt
      601 tttctgctaa agtgacaatt cataatttat atcactacta tgttcttatt cagctaaata
      661 aaataatcat tcctaaaagc cctttacttt ttaattcttt atttctcatt ccttatttct
      721 tatttctata cttgcccccc gcgacggtaa taaaaatatc ccccaattcc ccttagcttc
      781 cctccttaaa ccttatcgca tgaatgaacg attactccag tacatctggc agttccagta
      841 ttttgctgcc ggagaactct taacaacgac cggagaaccg gtgcaggtta tacagcaggg
      901 acagtataat accaatcagg gacccgattt ttcaagtgct aaaattaaaa tcgcaggtac
      961 tacctgggtg ggtagtgttg aactacatat acgttcatct gactgggaca aacacaagca
     1021 tcagcacgac cgcaattatg acaatgtaat cctgcatgtg gtgtgggaag aggatggatg
     1081 gaactacccg gtgccggtgc tggaattaaa aggccgggtt cctggattat tgctgaaccg
     1141 ttatgccctg ctgatggaga atcccggttt tattccctgt gaaaaaaata ttaccgggat
     1201 caatgaactc agctggacca gttggaaaga ccgcttgctg tcagaaagac tggaacgaaa
     1261 atcgggtttg atgcaacagt tcctggaaca gaatggaaat cactgggaag aagcaacctg
     1321 gtggatgatg gcccggaact ttggaataaa agtcaataca gaggcatttg agagtattgc
     1381 ccgttcatta cctgtttctt tactgtccaa acaaaagcac cagctcatcc tgctggaagc
     1441 cttgctgatg ggacaggcag gattgctcgg ggatgaaaag ccaggcgata aatattacac
     1501 cttgcttcaa cgggaatacc ggtttcagca aaccaaatac cagctccggc ctatcgcggt
     1561 tcccattcat tttctgcgga tgcgtcccgg aaattttccc accgtccgcc tggcccagct
     1621 ggcggtatta ctctttcaat ctgaatcact atttgccaaa ataaaagaaa cccggtccct
     1681 ggaggaatta aaacagtttt ttaatattac agcgaatgac tactggcatt atcattacag
     1741 ttttgacgaa gcctctccgt acaaacccaa aagactggga tcggtcatgc tggacaatct
     1801 catcattaat accctttgtc cgcttttatt tacctatggc agttaccacc gtgaacagga
     1861 gtataaagac cgggcggtgc aatggctaga acagacaacc gcagagtcca atacggtgac
     1921 ccggggatac agtcaattgg gtgttgctaa caaaaacgcc ttcgacagcc aggccctgat
     1981 cgaactgaag cagtcctatt gtgatcagaa aagatgcctt gaatgcgcag tgggggcgac
     2041 tctactaagg agtcaggagt cggtagtcgg gagtcggtag tcggtagtca atacaccttc
     2101 ttataaaatg tatctataat taacatgtaa tagttattca ggtttatcta atagtctcaa
     2161 ggtcttctga aaaatcagac tactaactcc cgactcccga ctactaacta gctccagctt
     2221 ccccagtcaa tattcacctc aatatcagga tggatactct tgatcaccgg gcaggtttcg
     2281 ccggtgtttt tgagaatctg ctttgttttt tcgtctattt ctttcagtga atcgggaata
     2341 tgaaatgtta aattaatccc gcccacacga cggggatcgg ctttcatgat tttcaggaca
     2401 tcgattctga cctcattcag gtcgaaaccc atggtccggg ccttgatgcc cataacggtc
     2461 accatacagg tagccaggct ggtggccatc aggtcggtgg gggagaaccg ttcgcccttc
     2521 ccgttgttat ccacgggggc atcggtttct acggcagact ggctttgcaa atgggtacag
     2581 gtggtgcgga gatcgccggt atatactacg gaagaagtca tgtcaatatt ttgccctaaa
     2641 tttacgttat agctaagaga t
//
LOCUS       DAOPMW010000438         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_48084_length_2642_cov_7.320062, whole genome shotgun sequence.
ACCESSION   DAOPMW010000438 DAOPMW010000000
VERSION     DAOPMW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48084_length_2642_cov_7.320062"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..745
                     /locus_tag="PLU11_14755"
     CDS             113..745
                     /locus_tag="PLU11_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPN60445.1"
                     /translation="MLNPQRILVLAPHTDDGELGCGGTISRFCTEGREVWYAAFCLCA
                     KSLPAHLPADTLVQECKKATAVLGIPPDRLLLFNYEVRELPQYRQQILEELLQLNKKI
                     NPDMVLLPAASDVHQDHQVIHQEGMRAFKNTTFAGYELPWNNYSFRTNYFMQLTEAEL
                     ERKTTSLKAYQSQSHRNYMQEDFTRSLAKVRGVQCNSLYAEAFEIYKLIS"
     gene            complement(746..1777)
                     /gene="porQ"
                     /locus_tag="PLU11_14760"
     CDS             complement(746..1777)
                     /gene="porQ"
                     /locus_tag="PLU11_14760"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPN60446.1"
                     /translation="MILHFIPAGAQVLGGRSVFNFLRLSHTPQLTALGGINISQPSDD
                     IGMTFNNPALFTAPMHTQVNAVFNDFYGGIKVFHLSLGYHHQPLNTDFTWGVNFFNYG
                     ATTETDAGGNVLGKFRPVDWVMQIGASRTYLEKWRYGATLKFISSNYGQYRSNGIATD
                     VALLYKDTAKMFTASFMIKNLGVQLRKYNNEQEELPFDMQVGITKRLSKAPFSFSLTA
                     HRLHQYDILYNDTSFNNNNGYPEVPSGFSVGKLFDHLVLGATIHIQEKVELQTGYNFL
                     RRRELNVGNSSNGLNGFSLGAGVFLGKFSLRYARSYYQASSAFNQIGFNLPLNQYFGP
                     GKSGEKTGW"
     gene            complement(1950..>2642)
                     /locus_tag="PLU11_14765"
     CDS             complement(1950..>2642)
                     /locus_tag="PLU11_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPN60447.1"
                     /translation="LDRQLASIMRYQAKELAMYEKVKTALTPDDIEVILGKARYSNDL
                     YKTANMAGVAVGLAWTYVGGDILFIETSISDGKGELKLTGNLGNVMKESASTALTYLQ
                     SNSKKYKLDPKFFEKKNIHIHVPEGAVPKDGPSAGVTMMTAIASAITGKRVKPFLAMT
                     GEITLRGQVLPVGGIKEKVLAAKRAGLKEIILCWQNEKDVQEIDSAFIKGIKFHYVKT
                     MNQVLELALDSK"
BASE COUNT          727 a          658 c          576 g          681 t
ORIGIN      
        1 ataagcaaaa atagcgattg ccggtcaatc atcggccggt ccgataaaac acccttcccc
       61 cattgattca tccgaccggc cgatgattga ccggcaatcg ctatttttgc ttatgctgaa
      121 tccacaaaga atactggtat tggcgccaca tactgatgat ggcgaactgg gatgcggtgg
      181 caccatttcc cggttttgca ccgaaggtcg tgaggtctgg tatgctgctt tttgtctttg
      241 tgccaaatca ctacccgctc atctgccggc cgataccctg gtgcaggaat gcaaaaaggc
      301 aacggcggta ttgggtattc caccggatcg actgttgtta ttcaattatg aagtaaggga
      361 actcccgcag taccggcagc agatcctgga ggaactgctt caactgaata aaaaaatcaa
      421 tccggatatg gttttattac ccgccgcttc ggatgttcac caggatcacc aggtcattca
      481 ccaggaagga atgcgggctt ttaagaatac cacttttgcc ggatatgaat taccctggaa
      541 taattacagc ttccggacaa attatttcat gcaactgaca gaagcggagc tggagcggaa
      601 aaccacttca ttgaaagctt accagtccca gtcgcaccgg aattatatgc aggaagactt
      661 tacccgatcg ctggcaaaag taagaggcgt acaatgcaac agcctgtatg cggaagcctt
      721 tgagatttac aaactgattt cctgattacc aaccggtttt ttcaccggat ttcccaggtc
      781 cgaagtactg atttaacgga aggttgaacc caatctggtt aaaagcagat gatgcctggt
      841 aataagaacg ggcataccgt aaactgaatt tgcccaggaa aacaccggcc cccaatgaaa
      901 atccattcaa cccgttacta ctattcccga cattcagttc acgacgccga aggaaattgt
      961 agcccgtctg taattccact ttctcctgta tatggatggt ggcgccaagc accaggtgat
     1021 caaacaattt accaacggaa aatccggacg gcacttccgg gtagccatta ttgttattaa
     1081 aactggtatc gttataaaga atatcatact gatgcagccg gtgagcggtc agtgaaaaac
     1141 taaagggcgc cttagacaat cgttttgtaa tacccacctg catgtcaaag ggtaactcct
     1201 cctgttcatt attatatttc ctcaactgaa cgccgaggtt tttgatcata aaagaagcgg
     1261 taaacatttt tgccgtgtcc ttatacaaaa gggccacatc ggtggcaatg ccattggaac
     1321 gatactgacc gtaattagaa ctgataaatt tcaacgtcgc cccataccgc catttctcca
     1381 ggtaggtacg ggaagccccg atctgcatca cccaatcaac cggacggaac ttccccagca
     1441 cattgccccc tgcatcggtc tcggtggtag ccccataatt aaagaaatta accccccagg
     1501 taaaatccgt attcaggggc tggtgatgat agccaagtga aagatgaaag acttttatgc
     1561 ctccataaaa atcattgaaa acggcattga cctgggtgtg catgggagca gtgaaaagtg
     1621 ccggattatt aaatgtcatt ccaatgtcat ccgatggctg ggaaatatta attccaccga
     1681 gtgctgttaa ttgtggtgtg tgtgaaagcc ttaaaaaatt aaacacggaa cgtcccccca
     1741 gcacctgtgc acctgccggt ataaaatgga ggatcattaa taggactaaa agccgccgca
     1801 tactcaacga aaataagtca tataaaaaga aaacccctcc gggaacagag gggatttcgt
     1861 gtttccgcat tgcggaacta accttaaaca accaacatgc accttaaata acagcacagg
     1921 gcaaagtttg gttgactgat tttaaaaaat tatttactgt ccagggccag ttcgagcacc
     1981 tggttcatgg tcttgacata gtggaattta atccccttaa taaaggcgga atcaatttcc
     2041 tgtacatctt tttcattctg ccagcaaagg ataatctcct tgagtccggc ccgttttgca
     2101 gccagtactt tttccttaat tcctcccacc ggcagtacct gtccgcggag ggtaatctct
     2161 ccggtcatgg ccaggaaggg tttcaccctt ttcccggtga tggcagaagc aattgccgtc
     2221 atcatagtaa ccccggcact gggaccatcc ttgggcaccg ccccttccgg gacatgtata
     2281 tgaatattct ttttttcaaa aaacttagga tccagtttat atttcttcga attactttgc
     2341 agataagtaa gggcggtact ggcactctcc ttcatcacat tgcccaggtt gcccgtgagc
     2401 ttcagttccc cttttccatc gctgatactg gtttcaataa acaggatatc cccacccaca
     2461 tatgtccagg ccaatccaac agccacccca gccatattgg cggttttgta cagatcatta
     2521 ctgtaccgcg cttttcccag tatcacttca atatcatccg gggtcagggc ggttttcact
     2581 ttttcataca tggccagttc cttggcctgg tagcgcatga tcgaagccag ctggcggtcc
     2641 ag
//
LOCUS       DAOPMW010000439         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_48559_length_2626_cov_4.476468, whole genome shotgun sequence.
ACCESSION   DAOPMW010000439 DAOPMW010000000
VERSION     DAOPMW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48559_length_2626_cov_4.476468"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..1658
                     /gene="glyA"
                     /locus_tag="PLU11_14770"
     CDS             381..1658
                     /gene="glyA"
                     /locus_tag="PLU11_14770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPN60448.1"
                     /translation="MQKDTLVFDLIRKELDRQRNGIELIASENFTSLAVIQATGSVPT
                     NKYAEGYPGKRYYGGCEVVDEIETLAIERLKKVFNASWANVQPHSGASANAAVFLAVM
                     KPGEKFLGLDLSMGGHLTHGSPANFSGKNYEALHYGVTKEGIVDYDQLEAKARAEKPK
                     MIICGASAYSRDWDYQRIRAVADEIGAVVFADIAHPAGLIATGLLNDPFEHCHIVSST
                     THKTLRGPRGGIIMLRHDFENPWGLKDPKGNLRTMCQLLDLAVFPGSQGGPLEHVIAA
                     KAVAFGEILSEDFKAYGQQIIANAQAMATSFINRGYQLISNGTENHLMLIDLRNKNLT
                     GKKAQETLDKAHITLNKNAVPFDDKSPFVTSGIRVGVPAVTTRGMKEAHMETVVELID
                     QVLMNADDENKIAGIKNEVRHFMQQFPLYPELG"
     gene            1811..2233
                     /locus_tag="PLU11_14775"
     CDS             1811..2233
                     /locus_tag="PLU11_14775"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPN60449.1"
                     /translation="MIQRQQTLWLLLATFAAIGSFMFPFVVGDEIRADNPMPVRASID
                     AGSSFLLLLATGASLVISTVTIFLYKNRQQQVWLAAGGMVITLLLLFLYIKEMNKLRE
                     PVLALSAVLPALLLFCYIMALRGIRKDEKLVKSLDKLR"
     gene            complement(2250..>2626)
                     /locus_tag="PLU11_14780"
     CDS             complement(2250..>2626)
                     /locus_tag="PLU11_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPN60450.1"
                     /translation="YSQADLQPAAQTYYRIKATELSGEISYSPVVKVISGDAGAETFS
                     IYPIPVQGKQFTLQYKGLPRENIQLQLFSNTGQVVYSNQWIHPGGQAAQTVELPANLS
                     RGMYYLQVSGENKRESRPVLIQ"
BASE COUNT          662 a          643 c          639 g          682 t
ORIGIN      
        1 atcaaaagcc aacgaaagta ccaattttag gcaaaaaaac gagtattagt ttggagatcc
       61 ccctgacagg ggatttagcc acggatgaca cggacctgcc tttgccggca ggcaggtcac
      121 acagatttac acggatttgg gccactaaaa ggatcaattg cagattgcct tcggcaataa
      181 aattggccac aaaaggacac aaaaaggcac gaaaaaatac cgccccttat taaaaacaac
      241 taaactaaca tagtaggggc tgttgactgc cgactgctga ctgccgactg ccgactgccg
      301 actgctcccc ccccactcac attttatcaa cgcctttttg tttaagtctt ttgaatcccg
      361 ttgaggctta attttgcggc atgcaaaaag acaccctcgt attcgatctg atccggaaag
      421 aactggaccg ccagcgtaat ggtattgaac tgattgcctc tgaaaatttt acttcactgg
      481 cagtgattca ggccaccggc agtgtgccca ccaataaata tgcggaaggc taccccggca
      541 aacgctatta cgggggttgt gaagtggtgg atgaaattga aaccctggcg attgagcgcc
      601 tgaaaaaagt atttaatgct tcctgggcga atgtgcagcc ccatagtggt gcttcggcta
      661 atgccgctgt atttctggca gtaatgaagc cgggcgaaaa atttctcggt ctcgatctca
      721 gcatgggcgg tcacctgacc catggcagtc cggccaactt cagtgggaag aattacgaag
      781 ccctgcatta tggagtgacg aaagaaggga ttgtggatta tgatcagctc gaggccaaag
      841 cccgtgcaga gaaacccaaa atgattatct gtggtgcttc cgcttatagc cgcgactggg
      901 attatcagcg gatccgtgca gtagccgacg agatcggcgc cgtggtattt gcggatatcg
      961 ctcacccggc cggactgatt gccaccggct tattgaatga tccttttgaa cattgtcata
     1021 ttgtttcttc cactacccat aaaaccttac ggggtccccg cggaggaatt atcatgctgc
     1081 gtcatgattt tgaaaatccc tggggcctga aagatcccaa gggcaacctg cgtaccatgt
     1141 gtcagttgct ggatctggcg gtatttccgg gctcacaggg cggaccgttg gaacatgtga
     1201 ttgcggcaaa agccgttgca tttggagaaa ttctttctga agattttaaa gcctacggcc
     1261 agcagattat agccaatgca caggccatgg ctacgtcctt tatcaaccgc ggttaccagc
     1321 tgatcagcaa cggtactgaa aatcacctga tgctgatcga tctccgtaac aaaaacctca
     1381 ctggtaaaaa agcacaggag accctggata aagcccatat caccctgaat aaaaacgcgg
     1441 taccttttga tgataaatca ccttttgtga catccggtat ccgggtaggt gtacccgcgg
     1501 taaccacccg tggcatgaag gaagcacata tggaaacggt ggtggagctg atcgatcagg
     1561 tgttgatgaa tgccgatgat gaaaacaaaa ttgcggggat aaagaatgaa gtaagacatt
     1621 tcatgcaaca gtttccgctg tatccggagt taggttgatt aggttgacca gttgacaggt
     1681 tgaccagttg acaaggaggt tttcttagaa gttccttgtc aacttgtcaa cctgttaact
     1741 tattaacttt gttacactcc ttacccagct gattccactt ataaactaat ttaaccaaaa
     1801 acactccccc atgatccagc gtcagcaaac cctttggcta ctgctcgcca cattcgccgc
     1861 aatcggaagt tttatgtttc ccttcgtggt tggagacgaa atccgggcag ataacccgat
     1921 gccggtccgg gccagcatcg atgccgggag tagtttctta ttattgctgg ctaccggggc
     1981 ttcattggtt atttccaccg taaccatctt tctgtataaa aacaggcaac aacaggtctg
     2041 gcttgcagca ggcgggatgg tgattacctt actgcttctt tttctctata taaaagaaat
     2101 gaataaactc cgggagcctg ttctggcctt gagtgcggta ttaccggccc ttctcctgtt
     2161 ctgctatatc atggcgctcc gcggcatccg gaaagacgag aaactggtta agtcactgga
     2221 taaattgcgc tgattccttc ctgttagtct tattgaatca gtaccgggcg gctttcccgc
     2281 ttattctccc cgctgacctg caggtaatac attcccctgg acagattggc cgggagctcg
     2341 acggtttgtg ccgcctgtcc gcccgggtgt atccattggt tactgtatac cacctgcccg
     2401 gtattgctga ataattgcag ctggatattt tccctcggca atcctttgta ttgcagggta
     2461 aattgttttc cctgcacggg aatgggatag atgctgaatg tttcggcacc tgcatctccg
     2521 ctgattactt ttaccacggg actgtaactg atttctccgc tgagttcggt cgcctttata
     2581 cggtaatacg tttgtgcagc cggctgcagg tccgcctggc tataac
//
LOCUS       DAOPMW010000440         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_48891_length_2613_cov_6.172791, whole genome shotgun sequence.
ACCESSION   DAOPMW010000440 DAOPMW010000000
VERSION     DAOPMW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48891_length_2613_cov_6.172791"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1089)
                     /locus_tag="PLU11_14785"
     CDS             complement(52..1089)
                     /locus_tag="PLU11_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPN60451.1"
                     /translation="MNRSHFIKQSTLLATGLACNPSWLFSKEGEDIILGHNNKRYRID
                     KKWGELDRNRYPVKDCHEMVQDSKGRIILLTNETKNNIIIYDKKGKLISSWGHEYPGG
                     HGLSLFNENGEDVLFICDNNRHQVIKTTLDGRILLTLNYPKETGEYSKEEEYIPTETT
                     IAPNGDIYVADGYGKDFIIQYNTKGEYIRHWGGRGTAEKHLKNAHGVCYDNRDKNNPV
                     LIVTSREEQAFKRYTTDGRYLDTIPVPGAWVCRPVIHGDYLYAAVLQSNSRLWQQSGF
                     ITILDKTNKVISNLSGSTPAYNGTMADEMYQTIKVFQYPHDVCIDDEENLYVAQWNSG
                     QAYPYKLKPVL"
     gene            complement(1121..2572)
                     /locus_tag="PLU11_14790"
     CDS             complement(1121..2572)
                     /locus_tag="PLU11_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPN60452.1"
                     /translation="MQKEYLEHRLNMNRRKFLSRLSLGVGSAALGSLLIPDLFSGSST
                     EEELLMTGLPHFAPKAKRIIYLFQNGAPSQLDLFDYKPRLQEMFGQDLPASIRMGQRL
                     TGMTADQAKFPLAGSVFNFKQHGQAGAWFSELLPHTASIADDICVVKTMFTEAINHDP
                     ALTFFQTGAQVGNRPSMGSWLSYGLGSENKNLPAFCVLLSKGKGNGQGVYSKLWTNGF
                     LDSIHQGVQFSSGEHPVLYLNDPESVSKEDRRKMLDKLGELNEVAYKEFGDPEINTKI
                     QQYEMAYRMQTAVPEITDMSKEPESIIKLYGPECLVPGTYAANCLLARKLSENGVRFI
                     QLYHQGWDGHNNLPGEIRGQCMDTDQASAALITDLKQRGLLDETLVIWGGEFGRTNYC
                     QGPLSKENYGRDHHPRCFSIWMAGGGVKPGVYGETDEFGYNIAKDPVHVHDFHATILH
                     LMGMNHEQLTYKHLGRRYRLTDVSGKLVNGLIA"
BASE COUNT          641 a          635 c          569 g          768 t
ORIGIN      
        1 tatacacagg ccggaaactg cagactaata ataaaatcat caggtaattc ctcatagaac
       61 cggctttaat ttataaggat atgcctgccc cgaattccat tgagccacat acaggttctc
      121 ttcatcatca atgcatacat cgtgcgggta ttgaaagacc tttattgtct ggtacatttc
      181 atcggccatc gtcccgttgt aagccggtgt gctgcctgac aggttggata taaccttatt
      241 ggttttgtcc agtatagtaa taaaaccgga ttgctgccat aagcggctat tgctttgcag
      301 tacggccgca tataaataat caccatgaat cacgggccgg caaacccagg cgcccggaac
      361 gggaatcgta tcaagatacc ggccatcagt tgtataacgt ttgaaagcct gttcttccct
      421 cgatgtcaca atcagtaccg ggttgttttt atcccggtta tcataacaga cgccgtgtgc
      481 attttttaaa tgtttttccg ctgtaccccg gccgccccag tgccggatat actctccttt
      541 agtattgtat tgtataataa agtccttgcc atagccatct gcaacataaa tatctccatt
      601 gggggcaatg gtagtttccg taggaatata ttcttcttct ttactgtatt cgccggtttc
      661 cttcggatag ttcagggtga gtaatatgcg gccatccagt gtggttttaa tcacctggtg
      721 ccggttgtta tcgcagataa acagcacatc ttccccgttt tcattgaaga gggacagtcc
      781 atgtccgccc ggatattcat gaccccagct gctgatcagc ttcccctttt tatcataaat
      841 gataatattg tttttggttt cattggtcag taaaatgatt cttcccttgc tgtcctgcac
      901 catttcgtga cagtctttta cgggataacg gttacgatcc aattcccccc attttttatc
      961 gatccggtaa cgtttattat tatgacccag aataatgtct tcaccttctt ttgaaaacag
     1021 ccatgagggg ttacaggcaa gtcctgtggc tagaagagtt gattgcttga taaaatgact
     1081 acgattcata ataaagatgt acgtcaggta aattgggtgt ttatgcaatc agtccgttga
     1141 ccagcttgcc cgaaacatcg gtgagccggt atcttctgcc aaggtgctta taggtcagtt
     1201 gttcatgatt cattcccatc aggtgtaaaa tagtggcgtg aaaatcgtgt acatgcaccg
     1261 gatccttagc gatattatag ccaaactcat cggtttcgcc atacaccccc ggcttaacac
     1321 cacccccggc catccagatc gaaaagcagc ggggatgatg atccctgccg taattttctt
     1381 ttgaaagcgg gccctggcaa taattggttc tgccaaactc accaccccat attaccagcg
     1441 tttcgtccag taatcctcgc tgtttaagat cagtgatcaa tgcggcactg gcctggtcgg
     1501 tatccatgca ctggccgcgg atttcaccgg gcagattatt atgtccgtcc cagccctggt
     1561 gatagagctg tataaagcgt accccgtttt cggaaagctt acgggcgagc aggcaattgg
     1621 ccgcataagt gccgggtact aaacattcag gtccgtataa tttgataatg ctttccggtt
     1681 ccttgctcat atccgtaatc tctggtacgg cagtctgcat gcggtaagcc atttcatatt
     1741 gctgaatctt ggtattgatt tcaggatcac cgaattcctt gtaagcgact tcattcagtt
     1801 cccctaattt atccagcatt ttccggcggt cttctttgga aacactttcc gggtcattta
     1861 agtataatac cggatgttcg ccactgctga actgaactcc ctggtgaatg gaatctaaaa
     1921 atccattggt ccataatttt gaataaacgc cctggccatt ccccttgcct ttggaaagta
     1981 aaacacaaaa cgcgggtaaa tttttattct cactgcctaa accgtagctc agccaggatc
     2041 ccatacttgg ccggttgccc acctgggcgc ctgtctggaa aaaggtcagt gccggatcat
     2101 gatttatcgc ttcagtgaac attgttttta ccacacatat atcatcggct atagaggcgg
     2161 tatgcggcaa tagctcactg aaccaggcac ccgcttgtcc gtgttgttta aaattaaata
     2221 ctgaaccggc cagtgggaat tttgcctgat cagcggtcat acccgtgagg cgttgtccca
     2281 ttcgtatcga ggctggcagg tcctgcccga acatttcctg taagcggggc ttgtaatcaa
     2341 acaaatcaag ctgtgaaggc gcgccatttt gaaagaggta aataatccgt ttggctttcg
     2401 gtgcaaaatg cggcagtccg gtcatcagca attcttcctc agtactactc ccactaaaca
     2461 gatctggtat taatagtgaa cccaatgccg cactgcccac gccgaggctg agcctggata
     2521 aaaacttgcg gcggttcata ttcagccggt gttctaaata ctctttctgc atgttcaggt
     2581 ttttgtaatg gcttcttcca ggttataaat ggt
//
LOCUS       DAOPMW010000441         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_51182_length_2532_cov_8.203472, whole genome shotgun sequence.
ACCESSION   DAOPMW010000441 DAOPMW010000000
VERSION     DAOPMW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51182_length_2532_cov_8.203472"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLU11_14795"
     CDS             <1..388
                     /locus_tag="PLU11_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60453.1"
                     /translation="VNLRVLDTLIKLAGLQPRQLPPSAILEQRKNELVKLLPDWTNAE
                     KSGIFAENFFPDYPIDSLKKEARDLFAKAGKIVEIKPMKPENQLRGSFIIHGEKADLE
                     VYFTLSPERPALVQEYHIREMPPSIR"
     gene            complement(478..1749)
                     /locus_tag="PLU11_14800"
     CDS             complement(478..1749)
                     /locus_tag="PLU11_14800"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN60454.1"
                     /translation="MKKLFLLLLIGFQIAAIAQPITLATPESQGFSSERLKRIDREMT
                     GWVDKGWMQGGAALVIRNNKIVYYKAVGYNDKELNVQMQKDQIFRIASQTKAITSVAI
                     MILFEEGKLLLDDPVSKYIPAFAKQQVLDKFNAADTTYTTVPAKKEITIRELLTHTSG
                     LGYAQIGSREANAIYAKSNITAGIGVKDDKLLDAMNRLAKLPLMHQPGEKWTYGLNTD
                     LLGCLVEVISGMSLDEFFRTRIFEPLAMTDTYFSIPKNKAWRLAKLYREDSTGKLVKA
                     EANMLNGKTVTPDYPLEGSTYYSGGAGLSSTIYDYGVFLQMLLNGGIHNGKRILSRNT
                     VRLMTMNQIGDLALGNDKFGLGFQVVTERGSARTPSNPGTFSWGGAFATSYWVDPKEK
                     MVFLFYRQLQSSTHGDVVEKFRAMLYGAMND"
     gene            complement(1867..>2532)
                     /locus_tag="PLU11_14805"
     CDS             complement(1867..>2532)
                     /locus_tag="PLU11_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60455.1"
                     /translation="LVQWGDNGATDKDWQFETVGTGNSQEGGRKILFDIMLNNIAVSE
                     ATRNQIDNGDCKRIFGVVSIELWELDNNNEMKTRLRSYNNMSELLYQQTNFRNPPTAA
                     LSKYQDNNNSNTQMSKVTYNIPENLLINKRLMLVVKTNVGSRHKDNDFASYDALKMKE
                     EVQSTFILDNRAARTETIETITDLSSSTRDMHFLDTMIPFAIFQKTDDTHKIWVKISC
                     KKN"
BASE COUNT          613 a          576 c          631 g          712 t
ORIGIN      
        1 agtaaacctt cgggtattgg ataccctgat caaactggcc ggattgcagc cccgtcaatt
       61 accgccatca gcgatattgg aacaacggaa gaatgaactg gtaaaactgc tgcccgactg
      121 gaccaatgcg gaaaagagtg gcatctttgc tgaaaacttc ttcccggatt atcctattga
      181 ttcattgaag aaagaagccc gtgatctctt tgccaaagcc ggaaagattg tcgagataaa
      241 accgatgaag ccggagaatc agctacgcgg ttcctttatc atccatggcg aaaaggctga
      301 cctggaagtg tactttacgc tgagtccgga acggccggcg ctggtgcagg agtatcatat
      361 tagggaaatg cctccttcga tacgctaagt gtgcatggtt cggcaagctc accatgacaa
      421 cgtctgtcac cctgagcctg cctaccggtc aggcaggctt gccgtagggt acaactacta
      481 atcattcata gccccataca acatcgccct gaacttttct accacatcac catgggtgga
      541 actctgtaat tggcgataaa agaggaagac cattttttct ttggggtcta cccagtagga
      601 agtggcgaag gcgccgcccc agctgaaagt gccgggattg gagggcgtgc gggcactgcc
      661 gcgttcggtg accacctgga aaccgagacc gaatttatca ttgcctaatg cgagatcccc
      721 gatctggttc atggtcatca ggcgaactgt gttgcggctg aggatgcgtt tgccattatg
      781 aatgccgcca ttgaggagca tctgcaggaa cacgccataa tcataaatgg tagaggataa
      841 accggcacca ccggaatagt aggtgcttcc ttccagtgga taatcgggag taaccgtttt
      901 accattcagc atattggctt cagcctttac cagtttaccg gtggagtctt cgcggtagag
      961 tttggccagg cgccaggctt tgtttttggg aatactgaaa taggtatcag tcatggccag
     1021 tggttcaaag atcctggtgc ggaaaaattc atcgagggac atgcctgaga ttacctctac
     1081 taaacaacct aacaggtcgg tattaagtcc ataggtccat ttttccccgg gctggtgcat
     1141 caggggcagt ttggccaggc ggttcatcgc atccagcagt ttatcgtcct tcacgccgat
     1201 accggcggtg atattcgatt tggcatagat ggcgtttgct tccctggaac cgatctgtgc
     1261 ataccctaat ccggaagtat gggtcaacag ttcacggatc gtgatttcct ttttggcggg
     1321 taccgtggta taagtagtat cggccgcatt gaatttatcc agcacctgtt gtttggcaaa
     1381 ggcgggaata tattttgaaa ccggatcgtc cagtaatagt ttgccttctt caaaaagaat
     1441 catgatggcc acactggtaa tggctttggt ctgcgaggcg atacgaaaga tctggtcctt
     1501 ttgcatctgc acatttaact ccttgtcatt atagccaacc gctttataat aaacgatctt
     1561 attattccgg atcacgaggg cggctcctcc ctgcatccat cctttgtcca cccaaccggt
     1621 catttcccgg tcaatccgtt tcagtcgttc actggaaaaa ccctgtgatt ccggtgtggc
     1681 aagggtaatg ggttgagcga tagcggctat ttgaaagcca atcagcagaa ggaggaagag
     1741 ttttttcata tgtgaagttg tgggttaaag ttaagggata aacaatcatt agttgtttat
     1801 cccttagagc aacttcatcg cttcgcaaga tctaggtccc tccttcgtcg ggatgacaat
     1861 gttatattag tttttcttgc aagaaatttt cacccatatc ttatgcgtat catctgtttt
     1921 ctgaaagatg gcgaagggga tcatagtatc caggaaatgc atgtcccggg tggatgaact
     1981 gaggtcggtg atagtttcaa tggtttctgt acgggcggca cggttatcca gtatgaaagt
     2041 ggattggact tcctctttca ttttcagggc atcgtaggaa gcgaagtcat tgtctttatg
     2101 ccggctgccc acattggttt ttaccaccag catcagccgt ttattgatca gcaggttttc
     2161 aggaatatta taggtcactt tgctcatttg tgtattgctg ttgttattat cctggtattt
     2221 gctgagggcc gcagtgggtg gattacggaa attggtttgc tggtacagga gttcggacat
     2281 attattatag gaacggagac gggttttcat ttcattgtta ttatccagtt cccagagttc
     2341 aatgcttacc actccgaaaa tccttttaca atcgccatta tcgatttggt tgcgggtggc
     2401 ttctgaaaca gcaatattat tgagcatgat atcgaacagt attttccggc caccttcctg
     2461 ggaattaccg gtacccactg tttcgaactg ccagtcttta tccgtggcgc cattatctcc
     2521 ccattgtacc ag
//
LOCUS       DAOPMW010000442         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_51213_length_2531_cov_6.735864, whole genome shotgun sequence.
ACCESSION   DAOPMW010000442 DAOPMW010000000
VERSION     DAOPMW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51213_length_2531_cov_6.735864"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1442)
                     /locus_tag="PLU11_14810"
     CDS             complement(186..1442)
                     /locus_tag="PLU11_14810"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN60456.1"
                     /translation="MKSFLILVLLATVFYCCNTSVKEKSVPEDSLQYYPPTPAPISKD
                     EFRQYYRQLSSFFDSTLLRYKNFNGQILIAKNGTILYEKYIGKRDLRKKDTMSSATSL
                     HIASTSKTFTGVAILRLAQEGKLSLDDSLSRYFPELPYPGITIKMLLNHRSGLPNYVH
                     FMDRSGWNKKVYCTNPDVLQFMINTPPNKEANPNRKFSYCNTNYLLLAMLIEKVTGKT
                     YPEYLREKFFLPLQMKDSYVFTLKDTLTATPSFKADGYIWTNDFLDGTYGDKNIYTTA
                     GDLLKWDQALYTDQVISKALLDSAFTSYSNERPSVHNYGLGFRLMNLPNNKKIVYHFG
                     KWHGCNAAFARLIDEKVTIIILGNRFTRSIYNTAHQSYDLFGEYFQKTSTEEEETDTL
                     PSKKSARLKEKGAGSLPVKTEKKARR"
     gene            1622..>2531
                     /gene="serA"
                     /locus_tag="PLU11_14815"
     CDS             1622..>2531
                     /gene="serA"
                     /locus_tag="PLU11_14815"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPN60457.1"
                     /translation="MTKTGNNTSYPKEKIRILFLENISDSAVKNFRHHGYVKVEKITK
                     ALTEEELIDEIKDVHILGIRSKTMVTKKVLEAAKKLQAIGCFCIGVNQVDLKSATKNG
                     VVVFNAPYSNTRSVAELVIGLAIMLIRRIPDKNKAAHEGIWNKDAKNSYELRGKTLGI
                     VGYGNIGSQVSVLAESLGMKVIFYDVETKLPLGNAEDARSLKELLGRADVVTLHVPET
                     SQTKNLINKNNLRYFKKGAILLNYARGEVVDLDALRKEIVEGNIGGAAIDVFPWEPEK
                     NGDRFQSPLQDLPNVILTPHIGGSTEE"
BASE COUNT          681 a          539 c          637 g          674 t
ORIGIN      
        1 cgcatctgtt ccgggtattg ccgcaacaac cattccgtac aggccgtagc cgccctttcc
       61 atcagttcaa ttgaatgaat gggctcgtgg gtgatcgtaa actgatccca gtcccggatc
      121 tgttgggccg taaaaatttt cattggagct taaaggtccg ccatttttcc gatatatgga
      181 atgacttatc gccgggcctt tttttctgtt ttaaccggta gagagccggc tcctttttct
      241 ttcaaacggg ctgatttttt ggaggggagc gtatcggttt cttcttcttc ggtactcgtc
      301 ttctggaaat attccccgaa gagatcataa ctttggtggg cagtattgta tatactccgg
      361 gtaaagcggt tgccgaggat aatgatcgtc actttttcgt ctatcagccg cgcaaatgcg
      421 gcattacaac catgccattt cccgaaatga tacacaattt ttttgttatt gggcaggttc
      481 atcagccgga aaccgagccc gtaattatga accgaaggtc gttcattact ataggatgta
      541 aaggctgaat ccagcagcgc tttactgatc acctggtcgg tgtataaggc ctgatcccat
      601 ttcagcaggt cgccggccgt ggtataaata tttttatcac catacgtgcc atccaggaaa
      661 tcattggtcc agatatagcc atcggctttg aatgaaggcg tggccgtgag ggtgtctttc
      721 aaggtaaaaa catagctgtc cttcatctgc aagggaagga agaatttttc ccttaaatat
      781 tccggatagg ttttaccagt cactttttca atcagcatgg ccagcaacag gtaattcgta
      841 ttgcagtaac tgaatttccg gttcgggttc gcctctttat tcgggggggt attgatcatg
      901 aattgcaata catccggatt ggtacaatat actttcttat tccagcccga ccggtccatg
      961 aagtgtacat aattgggcaa accgctccgg tgattcagca gcattttgat ggtgattccc
     1021 ggatagggta gttcgggaaa ataacgggac aggctatcat ccagtgataa ctttccttcc
     1081 tgtgccagcc gtaatatggc caccccggta aacgtcttac tggtggaagc aatatgcagg
     1141 gacgtagcgg acgacatggt atctttcttc cgcagatccc ttttcccgat atacttttca
     1201 tagaggattg tcccgttttt ggcaatcagg atctgaccgt tgaaattttt ataccgcagg
     1261 agggtactgt caaaaaagga agaaagttga cggtaatact gccggaattc atccttgctg
     1321 atcggagcag gggtgggcgg ataatattgc agggaatctt cgggaacgga tttttccttt
     1381 acagatgtat tacaacaata aaagaccgtg gccagcagga ccagtataag gaatgatttc
     1441 aaaagtgtag gttcgttggt ttgtttacaa aaataaaagc ctgatgcccc ggtcagggtt
     1501 atagttttcc ccaaccgctc tggtaatgtt aataataaaa caagtgttag tgttaatatt
     1561 ttgggcagga gtttccaata caagtgaaaa tgcagttgtt catacgtagt atatttgctg
     1621 tatgacaaag actggcaata acacgagtta tcccaaagag aagatccgca tccttttcct
     1681 ggaaaatatc agtgattcag cggtaaagaa tttccgccat cacgggtatg tgaaagtgga
     1741 gaagatcacc aaggcactaa cggaagagga actgattgat gaaatcaaag atgtacatat
     1801 tctcggcatc cgttccaaaa ccatggtgac gaaaaaagtg ctggaggcgg ctaaaaaact
     1861 gcaggccatt ggttgctttt gcataggagt taaccaggta gatctgaagt cagctaccaa
     1921 aaacggggta gtggttttca atgcacccta ctccaataca cgcagtgtgg ctgaactggt
     1981 gatcggactg gcgattatgc tgatccgccg gatcccggat aagaataaag cggcgcatga
     2041 gggcatctgg aataaggatg ccaaaaacag ttatgaatta agaggcaaga ccctcggtat
     2101 tgttggatat ggaaatatcg gcagccaggt cagtgtactg gcggaatcac tcggaatgaa
     2161 agtgattttt tatgatgtgg aaaccaaact gcccctgggc aatgcggaag atgcccgtag
     2221 cctgaaagaa ctgctgggcc gggccgatgt ggtcaccctg catgtgccgg aaaccagtca
     2281 gacaaaaaat ctgatcaata agaacaacct gcgttatttc aagaaagggg ccatcctgct
     2341 caactatgcc cgcggggaag tggttgacct ggacgcactc cgcaaagaaa ttgtagaagg
     2401 gaatataggt ggtgcagcga tcgatgtttt tccctgggag ccggagaaaa acggcgatcg
     2461 ttttcagtcc ccgttacagg acctgcccaa tgtgatcctg actccgcata tcgggggcag
     2521 caccgaagag g
//
LOCUS       DAOPMW010000443         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_51330_length_2527_cov_11.639563, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000443 DAOPMW010000000
VERSION     DAOPMW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51330_length_2527_cov_11.639563"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="PLU11_14820"
     CDS             <1..1905
                     /locus_tag="PLU11_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPN60458.1"
                     /translation="YGKAIFIEDNLTITPTACGGSSGSIEGLIVEGTEPLALEWYDGD
                     GNLVADALDIYNLAVNNYYLHVLDDHGCETVSEAYTITDAGDVEISGVDYSPGHCGQE
                     NGFIEITATTGSGTQLLYSIDNGNSWQTGNQFADLPAGAYFIKVGDQVGCETVYAGNP
                     VVIENIPGPDITAVTTTPENDYLADGSIDMEAIIGSGDLYYSVDNGSSFQTNNGLFEN
                     LSAGTYTCVVKDDFGCDTSFTVTIDRILSQLIDAIAGDGYTCIGDVTASQLLLNNFTE
                     VDSFHVQLTYDPAVIQCDGYIQIHPDLENGLQASLIPALGEVHITWKGNMPITLSANA
                     KMLELVFSGLEEGVSQVDWIAEPGEGQFFNVYGEEIAVQYELGTIRIFTRPEIILGIE
                     LHVCEGDNIYLPAFVMGGTGEYDYYWTGPSNFSSTDKELKLDGIQANQAGDYQLTVTD
                     TIGCTGQNTIKLTVSDNPVAAFHGSDTLEMQAGDVLDAGAGLSSYRWNTGNSTESIVI
                     NATGMYKVEMESPVGCLGSDSVYVKLTSEEIPETHLFIPNAFTPDGDGVNDTFQAFYN
                     GNDISHFTMEVYDRWGGRIFRSENILLGWDGKKNGKECPGGVYVYKIVFSLDGISGNQ
                     ERVGTVTLVR"
BASE COUNT          723 a          539 c          602 g          663 t
ORIGIN      
        1 tacggcaagg caatttttat tgaagacaac ctgaccatca cacccacggc ctgcggggga
       61 agcagcggca gcatcgaagg gcttatagtc gaaggaaccg aaccgcttgc ccttgaatgg
      121 tatgatggcg acgggaacct ggtggccgat gcattagaca tttataacct ggctgtgaac
      181 aattactatt tacatgtact cgatgaccat ggatgcgaaa cggtatccga agcctatacc
      241 attaccgatg ccggggatgt tgagatatct ggggttgact acagccccgg acattgcggc
      301 caggaaaacg gcttcatcga gatcacggcc actaccggtt ccggcacaca gttgctgtat
      361 tctatcgaca atggcaacag ttggcagaca ggcaaccagt ttgccgacct gccggcgggc
      421 gcttacttta ttaaagtcgg cgaccaggtg ggctgtgaaa ccgtttatgc cggcaacccg
      481 gtggtgatcg aaaacattcc tggcccggat atcacggcag ttactactac accggaaaac
      541 gattaccttg ccgatggcag cattgacatg gaagcgatca ttggtagtgg cgatctttac
      601 tattcagtcg ataacggaag cagcttccaa acaaacaacg gtctttttga gaatcttagt
      661 gcaggaacat acacttgtgt ggtaaaagat gactttggtt gcgatacatc ttttacagtg
      721 accattgaca ggatactctc gcagcttatt gatgccatag caggtgacgg ttacacctgt
      781 atcggcgatg taacggccag tcaattgttg ttgaataatt ttaccgaggt agatagtttt
      841 catgttcaac tgacctacga tccggccgtt atccaatgtg atggttatat tcagatccat
      901 cctgatctgg aaaacggatt gcaggcaagc ctgatccctg cgctgggaga agtccatatc
      961 acctggaaag ggaacatgcc catcaccttg tctgcaaatg caaaaatgct ggaactggta
     1021 ttcagcggac ttgaagaagg ggtatcccag gtcgattgga tagccgaacc gggcgaaggg
     1081 cagtttttca atgtttacgg agaagaaatt gctgtgcaat acgaactggg tacgatccga
     1141 atcttcacac ggccggagat cattttggga atagaattac acgtatgcga aggcgataac
     1201 atttacttac cggcatttgt catgggaggt acaggagagt atgattacta ttggacaggc
     1261 ccctcaaatt tttcaagcac tgacaaagaa ctcaagctgg atggaatcca ggcaaatcag
     1321 gctggagact accagttaac ggttacagac accataggat gcactggcca aaatactatc
     1381 aaacttaccg tcagcgataa tccggtagct gctttccatg gcagcgatac cctggaaatg
     1441 caggcagggg atgtgctgga tgctggagca ggcttatctt cctatcgctg gaataccggc
     1501 aacagcacgg aaagtattgt catcaatgct acaggaatgt acaaagttga gatggagtct
     1561 ccagtgggtt gtttgggttc agattccgtt tatgtaaaat tgacatcaga agaaataccc
     1621 gaaactcatt tgttcatccc caatgccttt accccggatg gagacggagt gaatgatacc
     1681 tttcaagcat tttacaatgg caatgacatt tcacatttca caatggaggt atatgaccgg
     1741 tggggaggac gaattttcag atcagaaaac atccttcttg gttgggatgg aaagaaaaat
     1801 ggaaaggaat gtccgggtgg agtgtatgta tacaaaatcg tattttccct ggatggtatt
     1861 tccgggaacc aggagagggt tgggacggtg acgctggtca gataacccgt tcactcttca
     1921 ccgctcacag ttcaccacat caaatcttta ataaaaatta ttggggctaa cggttggcgc
     1981 aactgccggg cagtgttttt ggccgggcag gcgggtattg catcaaagct taaaggtaag
     2041 aattaatttc tcaaaatgcc cgcctgggcg gacaagattt tttcaacact ggctggcagt
     2101 tgcgtttttg ttagccccag taatttcaaa tcgaaaatgg taaattaata gaaaagctat
     2161 tcgccctggc gcaccatctt tgaaagtaaa atttatcctt ttcaaaaata tttatcagac
     2221 gcacagtgca tttttcgaaa tttataatta tctttagctc cccccgatat gatggctaag
     2281 aaaataaaat attgtcaaat ttctcaccta tttgcgaatt cccatccgcc atgaagggcc
     2341 agcaaccctg agaaaattta aaaatcccga tcaatcaaac tcctttatta tcatttaaca
     2401 gctggattta gagaagaagt tccgatctgt actcgtattt atcaatttat ctatttttat
     2461 gcaagtatta atcctgatca attcaataaa agcataaagt tataacgttt ggattgaatt
     2521 tcttagc
//
LOCUS       DAOPMW010000444         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_54099_length_2439_cov_8.340185, whole genome shotgun sequence.
ACCESSION   DAOPMW010000444 DAOPMW010000000
VERSION     DAOPMW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54099_length_2439_cov_8.340185"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLU11_14825"
     CDS             <1..608
                     /locus_tag="PLU11_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN60459.1"
                     /translation="CNDRTFPGVSHTVYTNMDERQLVKSCLKGKPAAQKELYDRFAEQ
                     MLGVCYRYTKSLADAEDVLQDGFVKVYRFLHTYQFEGPLGAWIRRIMVTTALNFLKKH
                     SRYRTDLSFDNNDALHPVVENDAEVRMTARELAELIRQLPTGYQTIFNLHAVEGFTHV
                     EIGSMLGISDGTSRSQYARARSLLISWVEKKSQIKNDHYAG"
     gene            598..2100
                     /locus_tag="PLU11_14830"
     CDS             598..2100
                     /locus_tag="PLU11_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60460.1"
                     /translation="MRDNEFEKGVQEKMGEFRLPPSAPVWVEVERRIRERKRRRILIF
                     WFCLAGLLMAGFGGWWFWESNDRPALSITKEGSNSSVIRSTEKNTKDPREEIALPAKD
                     DKEQKINVADPSSSPSVGKDLSKINRGETTTGINAPLLVKSSSLKQLNKKTADKELTS
                     KAQLTAGSGKTTRQQTSPKNKVDKLVPDNDSQVATQKSTTEFPVASTSENKSTTEPPV
                     PATATLAVAVPVDISTAKPADTVQAVGNDKQQTTPSLRKSKKSKWQTGIVFAYGESRL
                     IRGGLSLFSEKSFDALQSGVSSGNGNFNNQSFADSVPLKGSGIHTGLFVKRQLGKKTS
                     FSAGLNWSYYSGKQRVGTRVDSIRQLSSPFNTDTRNGFYRSGNASRFTNRYYYLQLPL
                     LLHWQVNKGSNWPAIEWENGFVPSFLIASRALVYDPASRIFFRDKKVYNSFSLVYQTG
                     LTATLASRTKHPLTAGVYYNYHISRLQKTNPPDFNHQSSYGIQFRWLLKK"
     gene            2151..>2439
                     /locus_tag="PLU11_14835"
     CDS             2151..>2439
                     /locus_tag="PLU11_14835"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60461.1"
                     /translation="MRIRIFLLTVLTAFVFQSCLKDKMLYTYTIARPVYEQKSVVYAN
                     IRSNAPQQVEHPGKIYLYGNYIFLSEVDKGVHIIDNSNPANPVRKAFINIPG"
BASE COUNT          707 a          563 c          580 g          589 t
ORIGIN      
        1 cgtgcaacga ccggacattt cccggtgtca gtcatactgt atacacaaac atggatgaac
       61 gccaattggt aaaaagctgc cttaaaggca aaccagctgc ccagaaagaa ctgtatgacc
      121 gctttgcgga gcagatgctg ggggtctgtt atcgctatac caaatcgctg gccgatgcgg
      181 aagatgtgtt acaggatggc ttcgtgaaag tataccggtt cctgcatacc tatcagtttg
      241 aagggccact cggagcctgg atccggcgga tcatggtcac taccgccctg aattttctga
      301 aaaaacacag ccgctatcgt acggatcttt catttgacaa caatgacgcg cttcatccgg
      361 tagtggaaaa tgatgcagaa gtcagaatga ctgcccggga actggcggag ctgatccggc
      421 aactcccaac gggttaccag accattttca acctgcatgc cgtggaaggg tttacccatg
      481 tggaaatagg ctccatgctg ggaatctctg atggtacttc ccgttcgcag tatgccaggg
      541 cccgtagcct gctgatcagt tgggttgaaa aaaaatcaca gattaaaaat gaccactatg
      601 cgggataatg aatttgaaaa aggggtacag gaaaagatgg gggagttccg ccttccgcct
      661 tctgcaccgg tatgggtgga agtggagcgc aggatccgtg aacgcaaaag aagaagaatc
      721 ctgatcttct ggttttgcct ggccggattg ctgatggcag gattcggtgg ttggtggttt
      781 tgggaaagta atgaccggcc tgcactcagc ataacaaagg aagggagtaa ctcatccgtt
      841 atcagatcaa ctgaaaaaaa tacgaaagat cccagggaag aaatagcgtt gcctgcaaag
      901 gacgacaagg aacaaaagat caatgtagct gacccatcca gcagtccatc agttggaaaa
      961 gatttgtcaa aaatcaaccg cggtgaaact actaccggaa taaacgctcc attgctggtt
     1021 aaaagcagtt cccttaaaca actaaataaa aaaacggcag ataaagaact tacttcaaag
     1081 gcacaactca ctgctggttc aggaaagaca acaaggcagc agacctcgcc aaaaaataag
     1141 gttgataaat tagtcccgga taatgatagc caggttgcga ctcaaaagag tacaacggaa
     1201 tttcctgttg cttcaacatc ggagaacaaa agtactacgg aaccccctgt tccagccaca
     1261 gcaactcttg ccgttgcagt gcctgttgat ataagtacag ccaaacctgc agataccgtt
     1321 caggcggtcg ggaatgataa gcagcagacc acgccatcat tgcggaaatc caaaaaaagt
     1381 aaatggcaga ccggaatcgt attcgcttat ggagagtcca gactaatccg gggagggttg
     1441 tctttgttca gtgagaaaag ttttgatgcc ctgcaatcag gggtatcctc cggaaatgga
     1501 aacttcaata accagagttt tgcggactct gtgccgctca aaggttcggg catacatacc
     1561 ggtctttttg taaaacgcca gttaggaaaa aagacctcct tttctgcagg actgaattgg
     1621 tcatactatt caggaaaaca acgggtgggt acaagagtgg acagtatacg ccagttgagt
     1681 tccccattta atacagatac acggaatggg ttttaccgat ccggaaatgc ctccaggttt
     1741 acgaaccgtt attactacct gcagctgccc ttactcctgc actggcaagt gaataaaggg
     1801 tcgaattggc cggccattga atgggaaaac ggttttgtac cctccttcct gattgccagc
     1861 cgggcactgg tttatgatcc ggcaagccgg attttcttcc gggataaaaa agtgtataat
     1921 tctttcagcc tggtctacca gaccggactt acagccaccc tggcttcccg gaccaagcat
     1981 ccgttgacag caggtgttta ttacaattac catatctccc ggctgcagaa aaccaacccg
     2041 cctgacttca atcatcagag cagttatgga atccagtttc gctggctatt gaaaaaatag
     2101 ccgtgcaacg gtaattttaa ttgcattgtc aatctatcaa actgtttaat atgcgtatca
     2161 gaattttcct ccttactgta ttgaccgcat ttgtgtttca gtcctgcctc aaggacaaaa
     2221 tgctttatac ctataccatt gcccgtcctg tctatgaaca gaagtcggtg gtatatgcca
     2281 atatccgttc caatgccccg caacaagtgg agcatccggg taaaatctat ctctatggta
     2341 attatatttt cctgagtgaa gtagacaagg gtgtgcatat cattgacaat agcaatccgg
     2401 ctaatccggt ccgtaaggcc tttatcaata ttcctggta
//
LOCUS       DAOPMW010000445         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_55315_length_2401_cov_12.682012, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000445 DAOPMW010000000
VERSION     DAOPMW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55315_length_2401_cov_12.682012"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLU11_14840"
     CDS             complement(<1..361)
                     /locus_tag="PLU11_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60462.1"
                     /translation="MKKSLLSLLFLFTAWTLLYAQSPSGKYILVKDSDGKTPRGGAEI
                     SMTFTTGKFSLKAAMPGQTVTDNGTWKITGSNITIAFKEMEQGTKTGAWSLTNGTLTL
                     PFMMLSNGKGSSTWQQAG"
     gene            937..2397
                     /locus_tag="PLU11_14845"
     CDS             937..2397
                     /locus_tag="PLU11_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN60463.1"
                     /translation="MAKVKRYINADTVKLYVAADKKKTIAHLLWGDRVTYESTARATN
                     GCLKVKARGREGYVEKEFLGTEKLLELYFIDVGQGDGVLIKTPDEKHIMIDGGYKRRA
                     QQSGKNAADFIDWKFAKDYGMKSIELDAMIASHNDADHYGGLWDLINPAEEKEMDIPT
                     DKVNVKKFYHAGVAWMKKGSSRNLGPVKDKKLTLLVEDLADIKSLLKNERDGYLLQGE
                     WASFMQCLVDKDIPCERLSQKSGHLKEFEKPGFSIKVLSPIEVDYNGKPAYNNLGSHS
                     FNTNGHSVTLRVDYKAVKIMLTGDLNAASQQRIMEFYEAADNTKVLACDVAKSCHHGS
                     DDCSFEFLSHLGPGATVISSGDAEGHCHPRPSIVAASALTGHKQIHQDKVVTPLIYST
                     EISRSYKLGSPQELKRKDNGESLFPDTELSVTYLETNAGDLNPARRTKSFWDKKIVGG
                     IIYGLVNVRTDGETILCATMSEKSNEWDIKKFKTRF"
BASE COUNT          724 a          505 c          588 g          584 t
ORIGIN      
        1 tacccgcctg ctgccaggta gaactgcctt tcccattact cagcatcata aagggaaggg
       61 tcagcgtgcc gttagtgagt gaccaggcgc ctgtctttgt accttgttcc atttctttaa
      121 aagcaatagt gatattgctg cccgtgattt tccaggtgcc attatccgta acagtttgtc
      181 ccggcatggc cgccttaagg ctgaattttc ctgtggtaaa agtcatggag atctccgctc
      241 cgcccctggg tgttttgcca tcactgtctt tcaccaggat gtatttaccg gaaggggatt
      301 gggcatagag cagggtccag gcagtaaaaa gaaacagcag ggagagcagg ctttttttca
      361 taaaaaggga gtttgccggg aggccggtat ttggggacta aagtccgttt tttttcaggg
      421 gaatcgcatc ccctgaaaat gggatatttt agaaaaaaga accaagaacc aagatgccaa
      481 gacgccaaag aataatcaag aaccaagaaa ccaagaatcc aaagaataat caagaaacaa
      541 gacgccaaaa caagaaccaa gaaaccccac cttcgctaaa gctacggtgg gcaagcaaga
      601 tcaaattgca aatttcaaaa agcaccaata ttaaaattcc aacgcctgct aagggatatt
      661 gggttggttg acagtagctt gctgcatttt gggtatgaaa acctgaaaaa ccgtttaaaa
      721 acccttttct gatattattt aactgttagc tttaactgca agcagtgggg gaatggattt
      781 tatttattaa tttaatattc ttcacttagg cctgtttagt tgcaactttc cggatattga
      841 tttagcagtg gcattacacc ggaatgccgg atcaatattt ctgccgttga tcagcgtatt
      901 tattaatcag aaccattaac caataaacca tcctttatgg caaaagtaaa acgttacatc
      961 aatgctgaca ccgtgaaatt gtatgtggca gcagacaaga aaaaaacaat cgctcacctg
     1021 ctctggggag accgggtcac ttatgaaagt actgcgaggg ccacgaatgg ctgtctgaaa
     1081 gtaaaagccc ggggtcggga gggttatgta gagaaggaat ttcttggaac ggagaaactg
     1141 cttgaactct atttcatcga cgtggggcag ggggatggcg tactgatcaa aacgccggat
     1201 gaaaaacata tcatgatcga tggcgggtat aaacgcaggg cccagcagag cggaaagaat
     1261 gcagctgatt ttattgactg gaaatttgcc aaagattatg gtatgaaatc cattgaactg
     1321 gatgccatga tcgcctccca taatgacgca gatcactatg gtggtttgtg ggacctgatc
     1381 aatcctgcag aagaaaaaga aatggatata ccaaccgata aagtgaatgt gaaaaagttc
     1441 tatcatgccg gtgtggcctg gatgaaaaaa gggagttcac gaaacctggg tccggtaaaa
     1501 gataaaaagc tgaccctgct ggtggaagac ctcgcggata taaaatccct cctgaaaaat
     1561 gaaagggatg gttacctgct gcagggagag tgggcatctt tcatgcaatg cctggtggat
     1621 aaggacatac cctgtgaacg cttatcgcag aaatcaggtc acctgaaaga atttgagaaa
     1681 ccgggtttct ccatcaaagt cctttctcca atagaagtgg attacaatgg caaaccggct
     1741 tataataacc tgggcagtca ttccttcaat acaaatggcc atagtgtaac actcagggtc
     1801 gattacaaag cggttaaaat aatgctgacc ggagacctga atgcggccag tcagcaacgg
     1861 atcatggagt tttatgaagc ggccgataat accaaggtcc tggcctgcga tgtagcgaag
     1921 tcctgtcacc acggcagcga cgattgttcc tttgagtttc tctcacatct tggacctggt
     1981 gctacggtga tttcgtccgg cgatgcagaa ggtcactgtc atccacgtcc atcgattgtg
     2041 gccgccagtg ccctgaccgg acataaacag attcatcagg ataaagtggt tactccgctg
     2101 atctactcca ccgaaatttc aagaagctat aaactgggat caccccagga acttaaacgg
     2161 aaggataatg gtgaatcgct gttcccggac accgaacttt cagttacgta cctggaaacc
     2221 aatgccggtg acctgaaccc ggccaggcgc accaaatcct tctgggataa aaagatcgtg
     2281 ggaggtatta tttatgggtt ggtaaatgta cggaccgacg gcgaaaccat tctctgtgcc
     2341 accatgagtg aaaaaagtaa tgaatgggat attaagaagt ttaagacgag attctgaggg
     2401 a
//
LOCUS       DAOPMW010000446         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_55457_length_2396_cov_6.493806, whole genome shotgun sequence.
ACCESSION   DAOPMW010000446 DAOPMW010000000
VERSION     DAOPMW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55457_length_2396_cov_6.493806"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLU11_14850"
     CDS             complement(<1..340)
                     /locus_tag="PLU11_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN60464.1"
                     /translation="MDNQHIIIRVATPSDKVYSKTITDEMAASAAARGTGIAKRSPDY
                     IEQKIDEGKAVIAITSQNEWVGFCYIETWSHGEYVANSGLIVAPAYRKSGVAKSIKKR
                     IFNLSREKYPE"
     gene            648..1112
                     /locus_tag="PLU11_14855"
     CDS             648..1112
                     /locus_tag="PLU11_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60465.1"
                     /translation="MSNNPINSPEQSLAQPTVKKKSGFRRFLRILLLLLLIGLALFFY
                     FRYFFVFGEGSKAGELNFFVKKGYVFKTYEGRVIQTGYNSKVPGSIQSNEFEFSVVNE
                     RVAQQLMSNSGAFMELHYKEYLGALPWRGMSKYVVDSVISVQKINKAETPLP"
     gene            complement(1116..1826)
                     /locus_tag="PLU11_14860"
     CDS             complement(1116..1826)
                     /locus_tag="PLU11_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPN60466.1"
                     /translation="MSTIYLIPAILQEEGIGAIPPSITDAIKTCQVFFVENERSTRRY
                     FKQLWKQFLPGQEIIIDNYEWVNMAEPGAEDLFRKKIKEDKIIGIVSEAGCPGVADPG
                     QTLIAIAQAMNTPVKPLVGPSSILLALMASGMNGQQFKFNGYLPIDNGLRIKALKELE
                     AESAKKDCTQIFIETPYRNNQLIEAVLKNCHPDTRFCIAVDITGPAEWIKTKSVRQWQ
                     NEKTDIHKLPAIFLLHAR"
     gene            complement(1831..>2396)
                     /locus_tag="PLU11_14865"
     CDS             complement(1831..>2396)
                     /locus_tag="PLU11_14865"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HPN60467.1"
                     /translation="GVEVWLQKERSEWLSFFFVMTGMMLRLVLVLFYFSLFKYAILII
                     GSPLFTYLSERTEAIIDEKEHVFNWSSIRKDAKRGIRLAIRNAGWQMVYLVALILLSL
                     VPVVGWITPVIALFLECYYFGVSMLDYSFARNNFSLSQSMAFTGRHKGLAIGNGLLFY
                     AMHALIILAPAYAIIAATLSVNKVKNS"
BASE COUNT          636 a          532 c          559 g          669 t
ORIGIN      
        1 cttcggggta tttttcccgg gagaggttaa agattctttt cttgatggat ttggcgacac
       61 cgctcttgcg ataggccggg gctacgatca gcccactatt cgctacgtat tcaccatggc
      121 tccaggtttc gatataacag aaacctaccc attcattttg actggtgata gcgattacgg
      181 ccttgccttc atcaattttt tgttcaatat aatcaggaga acgcttggcg atacccgtac
      241 cacgggcagc ggcagaagcg gccatttcgt cggtaattgt tttagagtag accttgtcgg
      301 aaggtgttgc taccctgata ataatatgtt gattgtccac tattgtaacg gtctggttat
      361 gagacgggcc accaatcctg aataattttc tcgggggaat tcttcactga cagggaccat
      421 ggtgagcggt tcgtcctgtc agtaaacagg gcgacgtcgg ggccgtgaaa aggtaaacac
      481 atcaaatcca acggaatata ccccgctggt gatgggcgcg tggaacgcaa attgcttggt
      541 atgtgtcaga atattcgcca ttgggctgaa taggttgtaa cttggacgca aatttataat
      601 aataattggt ttttaagacc aaaaagtttt gttataaaac actaagtatg agcaacaacc
      661 ctatcaattc acctgaacaa tcgttagccc aacccactgt aaagaaaaaa tcaggtttcc
      721 gtcgttttct ccggatctta ctcctcctgc tactgattgg cctggcactg tttttttatt
      781 tccggtattt ctttgttttc ggtgaaggca gtaaggcagg tgaactgaat ttctttgtga
      841 agaagggata tgttttcaag acctatgaag gccgggtgat ccaaaccggc tataattcaa
      901 aagtgccggg aagtattcag tccaatgaat ttgaattttc cgtggtgaat gaaagagtgg
      961 cccaacaact gatgtctaac agtggtgctt ttatggaatt acattacaaa gaatacctgg
     1021 gtgcgctgcc ctggagaggc atgagtaaat atgtggtgga tagtgtgatt tcggtgcaga
     1081 aaataaacaa agctgaaacg cccttgcctt aaccatcaac gtgcatgtaa taaaaatatg
     1141 gcaggtagtt tatgaatgtc tgttttttcg ttctgccatt gccgcactga ttttgtttta
     1201 atccattcgg ccggacctgt tatatccacg gcgatacaga aacgggtatc cggatggcag
     1261 ttcttaagta cggcttcgat aagctggttg ttccggtatg gtgtttcaat aaagatctgt
     1321 gtacagtctt tttttgctga ttcagcttcc agttctttca gtgcttttat ccgcaatcca
     1381 ttgtcaatag gtaagtagcc attgaattta aactgctggc cgttcatccc gctggccatc
     1441 agggcgagga ggatcgaact tggccccaca agtggcttaa cgggtgtatt catggcctgt
     1501 gctattgcga tcagggtttg acccggatcg gccacacccg gacacccggc ttcactcaca
     1561 atcccgataa tcttgtcttc ctttattttt ttccgaaaca ggtcttctgc tccgggttcc
     1621 gccatattca cccattcata gttatcaatg ataatctcct gaccgggcag gaactgtttc
     1681 cataattgtt tgaaataccg tcgggtgctt cgttcgttct ccacaaaaaa aacctggcat
     1741 gttttgatgg catcggtgat tgatggtggt atagcaccaa tgccctcttc ctgtaaaatg
     1801 gcggggatca gataaatggt ggacatcagt tcagctgttt ttaactttat taacactcag
     1861 ggtagccgca atgatagcat aggccggggc aagaatgatc agggcgtgca tcgcataaaa
     1921 gaggagtccg tttcctatgg ccagtccttt gtgacgaccg gtaaaagcca tactctgtga
     1981 cagactgaaa ttattccggg caaaagaata atcaagcatg gatacgccaa aataataaca
     2041 ttccaggaac agggcgatga ccggggtaat ccagcccact acgggtacga gggatagcag
     2101 gatcagggca accaggtaca ccatttgcca gcccgcgttt cggatagcca gccgtatacc
     2161 acgtttggca tctttccgga tggaagacca attgaagaca tgttcctttt catcgatgat
     2221 cgcttcagtt ctttcactca ggtaagtaaa aagcggggaa ccgatgatca ggatcgcata
     2281 tttaaacaac gaaaaataaa aaagaaccag taccagccgc aacatcatgc cggtcattac
     2341 aaaaaagaaa ctcagccatt cactacgctc cttctgtaac cagacttcca ccccca
//
LOCUS       DAOPMW010000447         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_56306_length_2372_cov_3.070350, whole genome shotgun sequence.
ACCESSION   DAOPMW010000447 DAOPMW010000000
VERSION     DAOPMW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56306_length_2372_cov_3.070350"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1305)
                     /locus_tag="PLU11_14870"
     CDS             complement(115..1305)
                     /locus_tag="PLU11_14870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN60468.1"
                     /translation="MLFVKILFWVMIAIVLYTYIGYGLLLYLLVKLKRIFKKSTPAVN
                     KVSYEPEVTLFIAAYNEKDYVADKMKNTLGLDYPKDKLRIVWVTDGSDDGTPELLKQY
                     KEAIVLHENGRNGKIGAMNRGMKLVKTPIVIFSDANTMLGKDSIRRIVRLFETPTVGC
                     VSGEKRITDKDTDVASGAGEGLYWKYESTLKKWDAELYSVVGAAGELFAIRTALYREV
                     EKDTLLDDFIISLRVAQEGYTIQYDPEAYAVESASANVKEELKRKIRISAGGIQSVVR
                     LRSLLNIFKYGTLSFQYISHRVLRWTLTPLCLVLLIPAGFALAWSDGLLSPGLYMILF
                     WLQLSFYVMALTGWFLENRSTSIKVLFVPYYFFIMNLSVVLGFMRYLKKSQTVNWERA
                     KRAA"
     gene            complement(1340..>2372)
                     /locus_tag="PLU11_14875"
     CDS             complement(1340..>2372)
                     /locus_tag="PLU11_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN60469.1"
                     /translation="VEVTASSYPAWEQLALPRAAEQAGCEILHCTSNTAPANCSVPLV
                     LTLHDIIYMERSYSGMLTGSSSSYQKFGNIYRKLIVPGIMKKSKKIITVSHFEKNRIA
                     DFFGMNTDSRLTAVYNGVSTHFKPVTDTDELKRVKKLYHLPDHYFFFLGNTDPKKNTR
                     GVLESFAAFRKQHPTDYKLVMLDYDRKELASLLNDIGSPDLAEHIILTGYVKNTDLPA
                     IYSQCQAFLYPSLRESFGIPMLEAMACGVPVITSNTSSMPEVAGDAAMIVDPYKPAEI
                     TEALLQICNDREYAATLVNRGIKRAADFSWKAMAQDVLKIYYKTVASIRYNKLMRKLD
                     LENPVPAIR"
BASE COUNT          615 a          547 c          554 g          656 t
ORIGIN      
        1 ctagtgtaga tttaacatgt accgggtatg atacaatcat cctgcccccg gaattaatca
       61 acatgcagct ttatataaat gtagtggtgt ttaccggttg gaatacgatt ctggtcaggc
      121 cgctcttttt gcgcgttccc agtttaccgt ttgtgatttt tttaagtagc gcataaaccc
      181 aagtacaacg gacaggttca tgatgaaaaa gtaatacggt acaaagagta ctttgatact
      241 ggtggatctg ttttcgagaa accagccggt aagtgccatg acataaaagg aaagctgtag
      301 ccagaagaga atcatataca agccaggact gagcaaacca tcggaccagg cgagtgcaaa
      361 tcctgccgga atcagtaata cgaggcagag cggggtgagc gtccagcgca gtacccgatg
      421 tgagatatac tggaaggaaa gggtgccgta tttaaaaata ttcaacaggg aacggaggcg
      481 aaccaccgat tgtattcccc ctgcagaaat ccgtatttta cgtttgagtt cttcctttac
      541 attggcagag gcggattcca cggcataggc ttcaggatca tattggatgg tatacccttc
      601 ctgggccacc cgtaatgaaa taataaaatc atcaagcagg gtatcttttt ccacttcccg
      661 gtacaaggcg gtccggatag caaagagttc accggcagcg cccactactg aatataactc
      721 ggcatcccat tttttaagtg tggattcata tttccagtac aatccttcac cggctccgga
      781 cgccacatcc gtatctttat ccgtgattct tttttcgccg gatacacagc ctacggtcgg
      841 ggtttcaaac aagcggacaa tccggcgtat ggaatccttt cccagcatgg tattggcatc
      901 gctgaaaatc acgatcggcg tttttacgag tttcatcccc cggttcatgg ccccgatttt
      961 accattccgc ccgttttcat gcaaaacaat ggcttccttg tattgcttta acaactccgg
     1021 ggtcccgtca tctgatccat cggtcaccca tacaatccgg agcttatctt ttggataatc
     1081 cagccccagt gtatttttca ttttatcagc cacataatct ttctcgttat aggcggcaat
     1141 aaaaagggtg acttccggct catagctgac cttgtttacc gccggtgttg attttttgaa
     1201 aattcttttc agttttacca gcagataaag caataggcca tagccgatat aggtataaag
     1261 aacaatggcg atcatgaccc agaaaagaat ttttacaaag agcatatttc agcatttagt
     1321 atgttttcaa cggatttgtt tatctgatag ctggtacggg attctcgaga tccaatttgc
     1381 gcattagttt attgtacctg atcgatgcaa ccgtcttata atagattttc agtacatcct
     1441 gcgccattgc tttccaggaa aaatcagctg cccgtttgat gccccggttg accagggtag
     1501 cggcgtattc cctgtcgtta caaatctgta acagggcttc tgttatttca gccggtttat
     1561 atggatcaac gatcatggcg gcatcgcccg ctacttccgg catcgatgaa gtgttggacg
     1621 tgatcacggg cacaccacat gccattgctt ccagcattgg gataccaaaa ctttcacgga
     1681 gtgaaggata caggaaggcc tggcactgac tgtaaatagc cggcaggtcg gtgtttttta
     1741 cataaccggt caggataatg tgctcggcca gatccggact gccgatatca ttcagcaggg
     1801 acgccagttc tttccggtca taatccagca tcaccagttt ataatcggtc gggtgctgtt
     1861 ttctgaatgc ggcaaaggac tccagtacac cacgggtgtt tttcttgggg tctgtattgc
     1921 caaggaaaaa gaaataatga tcaggcaaat gatacaattt cttcacccgt tttaattcgt
     1981 ctgtatccgt tacaggttta aaatgggtgc tgaccccgtt ataaacggcg gtcagccggg
     2041 aatcggtatt catgccaaag aaatcagcga tcctgttttt ttcaaaatgg gaaacggtaa
     2101 tgatcttctt gctttttttc ataatgccgg gtacgatcag tttacggtag atattaccga
     2161 atttctgata agaacttgac gatccggtca gcatacccga ataggagcgc tccatataaa
     2221 taatgtcatg cagggtcagt accagtggta cgctgcaatt agccggtgcg gtattactgg
     2281 tgcaatggag gatttcacat ccggcttgtt cggcagcacg cggcagggca agctgctccc
     2341 aggccgggta ggaggaggcc gtcacctcta cg
//
LOCUS       DAOPMW010000448         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_57882_length_2326_cov_13.102158, whole genome shotgun
            sequence.
ACCESSION   DAOPMW010000448 DAOPMW010000000
VERSION     DAOPMW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57882_length_2326_cov_13.102158"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLU11_14880"
     CDS             complement(<1..908)
                     /locus_tag="PLU11_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60470.1"
                     /translation="MGIHQTVKNILAGFIIIIAGNVLFAQTPDSDADGVPDSEDLCPT
                     TKGTKANKGCPDAAAGNKTTNYFAKDDFTTILDVICKSELAKYRHPQVPAQQLEGVAT
                     TLPQTGVNKQFPVYYSTDGKGSFIAMLILSNQVNEFAKAVDFVQQQLLYNSSSCLAYK
                     LFKPVPTPSGDTTFFAFNQNDEGPLMQIKVYKHRISASEIYVILGVETVPRRAPPVAK
                     VADPALCNDLLKIMEASSEGFKKVRSNPKKNSISGLDDYETPLPGLGLEKKKLRVEDW
                     YDVMELKEHKIITYDASSFVANKEKA"
     gene            complement(938..>2326)
                     /locus_tag="PLU11_14885"
     CDS             complement(938..>2326)
                     /locus_tag="PLU11_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60471.1"
                     /translation="DAWFWDFKDAGKFFRPQFIGNKSYIIYKDYEGLLETNGTASGTR
                     VLTSTGGKGEIFDVVATEKFLYMLTRSKSTDAKLYHYRLFRIDPVTDQTIQVTDPASG
                     KTVQFNREIEGGDMSYRLSAYKNRVIFVFFLEAGILPFKSLSVLHDEDPVPAVHYLVY
                     ETDMKLSGENLWKRIAVTNDKVYYYDSANYKVQIKEFRKTNGNNDRPDYYDFAGFDFA
                     KNGIRILSLFDNTNGEAFMLGAYEKGADKGRHFVLQFKDRKAAVLAKFESNLDEVVVN
                     NGTIYLFGYRRVVELKMDGTLQPVLSLQGENGLTPIGENRKMLLFQGARLFYGISADL
                     GSMLRTEYWCHDLAANKDYKIATVDMESMYNDKVRERKWLHVAGDYFYFLEPKQAYYM
                     LKRKNFITGAPEEEAVITSFDGTSASGNVGDIHQAGQVIFFWGNFTYKKKVKKKSKEF
                     KNRVIGAVSLQG"
BASE COUNT          569 a          609 c          452 g          696 t
ORIGIN      
        1 tccgcctttt ccttattcgc tacaaaactg ctggcatcat aagtgattat tttatgctcc
       61 ttcagctcca tcacatcata ccagtcctcc acccggagtt ttttcttttc caggcccaga
      121 ccgggcagag gggtttcata atcatccagt cccgaaatac tgttcttctt cggattgcta
      181 cgcacttttt taaacccttc actgctggct tccatgattt tcagcaggtc attacagaga
      241 gcagggtcgg ccacttttgc tactggcggc gcacgacggg gtacagtttc cactcctaat
      301 atgacataga tctcactggc actgatccgg tgtttataca ctttgatctg catcagcggc
      361 ccttcgtcgt tctggttaaa ggcaaaaaaa gtagtatcgc ccgagggggt tggtaccggt
      421 ttaaacaact tgtatgccag gcaggaagaa gaattgtaca gcagttgttg ctgtacaaaa
      481 tctaccgcct tggcaaactc attgacctga ttactcagga tcagcatcgc gataaagctc
      541 ccctttccat cggtcgaata atacacggga aattgtttgt ttacgcctgt ttgcggtaag
      601 gtagtggcta ctccttccag ttgttgcgcc ggcacctgcg gatgccggta tttcgccagc
      661 tccgatttac agatcacatc gaggatagtc gtaaaatcat ctttggcaaa ataattggtc
      721 gtcttatttc cggcagctgc atccggacaa cctttatttg ccttcgttcc ttttgtggtg
      781 ggacaaagat cttcgctgtc gggcaccccg tccgcatcgc tgtcgggcgt ttgggcaaaa
      841 agaacatttc cggctattat aattataaag ccagccagta tatttttcac tgtttgatgt
      901 atccccatag ttgttcagat taactgaatt ctcaccctca cccctgcaaa ctgactgctc
      961 cgatgacccg gtttttgaat tcttttgatt tcttttttac tttcttttta tacgtgaaat
     1021 ttccccaaaa gaaaatcacc tggcctgcct ggtgaatatc ccccacgttt ccgctggcgg
     1081 atgtgccgtc aaaactggta ataacggcct cttcttcagg agcacccgtt atgaaatttt
     1141 ttcgcttcag catataatag gcctgtttcg gttccaggaa ataaaaataa tctccggcca
     1201 catgcagcca cttgcgttcc cgaactttat cgttatacat actctccata tccaccgtgg
     1261 cgattttata gtccttgttt gctgccaggt cgtgacacca gtattccgtc ctgagcatac
     1321 tgcccaggtc cgcactgata ccataaaaca atctggcacc ttgaaacaat aacatttttc
     1381 tattttctcc aattggggtg agtccatttt ccccttgcag cgataataca ggttggagtg
     1441 tgccatccat cttcaactcc accacccgcc ggtatccaaa caaataaata gtgccgttat
     1501 ttaccaccac ctcgtccagg ttactttcaa attttgccag caccgcggct ttcctgtctt
     1561 taaactgaag tacgaagtgc cggcctttat cggccccttt ttcgtatgcg cccagcataa
     1621 acgcttcccc gtttgtgttg tcgaacaggg ataggatacg gatgccattc ttcgcaaaat
     1681 caaatcccgc aaaatcataa taatcgggac gatcattatt tccattggtc ttcctgaatt
     1741 ctttgatctg cactttataa ttagccgaat cataatagta cactttgtca ttggtgaccg
     1801 ctatccgttt ccaaagattc tccccgctca atttcatgtc tgtttcataa accaggtaat
     1861 ggaccgcggg taccgggtct tcgtcatgaa gcaccgacag acttttaaaa ggcaatatcc
     1921 ctgcttccag aaaaaacaca aagatcaccc tgtttttata agcactcagg cggtaagaca
     1981 tatccccacc ttctatctcc ctgttaaact gaacggtctt tccggatgca gggtcagtta
     2041 cctgaatggt ctgatccgtt accggatcga tacgaaacag cctgtaatga tagagcttag
     2101 catccgttga tttactacgc gtcaacatat agagaaattt ctctgtagca accacatcga
     2161 aaatctctcc ttttcccccg gtactggtta aaacccgggt accgctggct gtgccatttg
     2221 tttccagcaa accttcatag tctttataaa tgatataact tttattgcca ataaactgtg
     2281 gcctgaaaaa tttgcctgca tccttaaaat cccagaacca ggcgtc
//
LOCUS       DAOPMW010000449         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_61670_length_2226_cov_7.374021, whole genome shotgun sequence.
ACCESSION   DAOPMW010000449 DAOPMW010000000
VERSION     DAOPMW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61670_length_2226_cov_7.374021"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLU11_14890"
     CDS             <1..753
                     /locus_tag="PLU11_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPN60472.1"
                     /translation="ESSSRSNIEIPQAQKDLLNALLKTGKPVVLVLFTGRPLAIKEES
                     EKVPAILNVWFGGSEAGFAIADVLFGDVNPSGKLTTTFPQNVGQVPIFYAHKNTGRPL
                     EEGKWFSKFRSNYLDVSNDPVYPFGYGLSYTSFAYSDISLSSTSLKGNQKLTATVTVT
                     NNGKYEGKEVVQLYIRDVVGSVTRPVKELKGFQKISLKPGESKQVIFTITPEDLKFYN
                     YDLKFDWEAGDFQIMIGTNSKDVKMAKVNWVK"
     gene            901..2202
                     /locus_tag="PLU11_14895"
     CDS             901..2202
                     /locus_tag="PLU11_14895"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN60473.1"
                     /translation="MQRIEIQQLKKWKQSNGRKPMIIRGARQVGKTWLMKEFGRLEYK
                     DVVYVNCEADPAVRNMFTGDFDTTRIIRGLQIIAGKPIEPNHTLIIIDEIQEAPGALT
                     SLKYFQENAPEYHVIAAGSLLGITLNRSGSFPVGKVEFLDLFPLSFVEFLDAIHETQL
                     ADLLRNPDWSLVASFSIKFTDRLRQYYYTGGMPEVVLQYSVSGDMNQVREIQKRILVS
                     YEQDFSKHAPAEIVPRIRMIWSGVPAQLAKENRKFIYGQIKAGARAKDFELALSWLID
                     CGLLYKVHNVSKPALPLKAYEELNTFKLFMLDIGLLGALTELDARTLLEGNTVFTEFK
                     GALTEQYVYQQLRTMNDLPVYYWSAGNARSEVDFLLQHNGQIIPLEVKAEENLHAKSL
                     RVFCEKYKPALAVRSSMSGYRKEDWMTNLPLYAVSTITQLQ"
BASE COUNT          679 a          414 c          528 g          605 t
ORIGIN      
        1 gagagcagca gccgcagcaa tattgaaatt ccgcaggcac agaaagattt attaaatgcc
       61 ttattaaaaa ccggcaaacc ggtggtgctg gtcttattca ccggtcgtcc gctcgctata
      121 aaagaggaaa gtgagaaagt accggctatt ctgaatgtat ggtttggtgg cagtgaagcc
      181 ggctttgcga ttgccgatgt attattcggg gatgtaaatc cttccggcaa actgactaca
      241 acctttccgc agaatgtagg acaggtgccg atcttctatg cccacaagaa taccggccgt
      301 ccgctcgaag aaggtaaatg gttttccaag ttccgttcca actacctcga tgtaagcaat
      361 gatcccgttt atccctttgg atatggatta agctatacca gttttgcgta cagtgatatc
      421 agtctcagca gcacgtcgct gaaagggaat caaaagctga ctgctacggt tactgttacg
      481 aataatggga aatatgaggg taaggaagtc gtacaacttt atatccgcga tgtggtgggc
      541 agtgtaaccc gtccggtaaa agaactgaaa ggtttccaga aaatcagtct gaaacccgga
      601 gagtctaaac aggtgatttt cactattact cccgaagacc tgaagtttta caattatgac
      661 ctgaaatttg actgggaggc cggagatttt cagattatga ttggcactaa ttcgaaggac
      721 gtgaagatgg cgaaagtgaa ttgggtaaaa taggccactt agactacaaa ctccttagaa
      781 ccggcagtat tggtaacata ttgccggttt tttcgtgctt aaaaatgtga tattattcaa
      841 aaactcgtgc ttaaaaatgt gatatttagc ataaattcgt acgtataaat gtattgaaaa
      901 atgcaaagaa ttgaaataca gcaacttaaa aaatggaaac agtctaatgg tcgtaagccc
      961 atgattatcc ggggagcgag gcaggttggt aaaacctggc tgatgaaaga atttggaaga
     1021 ctggagtata aagacgtggt atatgtcaac tgtgaagcag atccggcagt acgaaacatg
     1081 tttacgggtg attttgatac aacaaggatc atccgcggat tacaaattat agccggaaaa
     1141 cccattgaac cgaatcacac attaatcatc attgatgaga tacaggaagc gcccggtgcc
     1201 ctgacttcat tgaagtactt tcaggaaaac gcgccggagt atcatgtaat agctgcaggc
     1261 tctttgttgg gaattacact taatcgttca ggatcattcc ccgttggtaa agttgaattc
     1321 cttgatttat tccccctgag ttttgttgag ttcctggatg ccattcatga aactcagtta
     1381 gcggatcttt tacgaaatcc ggattggtca ttagttgctt ctttcagtat aaaatttacg
     1441 gatcggttaa ggcaatacta ttataccggt ggtatgcccg aagttgtatt acaatattcc
     1501 gttagcgggg acatgaacca ggtaagagag atacagaagc ggatacttgt ctcgtatgaa
     1561 caggattttt cgaagcatgc gccggctgaa attgtacccc gtatccggat gatctggagc
     1621 ggggtccctg cacagctggc aaaagaaaac cggaaattta tttatggtca gataaaagcc
     1681 ggtgcaagag caaaggattt tgaactggcg ttatcctggt tgatcgattg cggattactg
     1741 tataaagtac ataatgtatc taagccggca ctgcccctga aagcatatga ggaattaaat
     1801 acatttaaac tttttatgct ggatataggt ttattggggg cgcttactga actggatgca
     1861 agaacactgc tggaaggcaa tactgtcttt actgaattca aaggagcgtt aacagagcag
     1921 tatgtttatc agcaattacg cacaatgaat gatcttccgg tttattattg gtcggcaggg
     1981 aatgcccgtt cagaggtgga ttttctttta cagcataatg gacaaatcat tccattggaa
     2041 gttaaggcag aagaaaacct gcatgcgaag agtctccgtg ttttttgcga aaaatataaa
     2101 ccagcacttg cggttcgtag ctccatgtcg ggctacagaa aagaagactg gatgacaaat
     2161 ctgccacttt atgcggtgag tacaattaca cagttacagt aaaaagtaat gaaagaaaat
     2221 atttct
//
LOCUS       DAOPMW010000450         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_61793_length_2223_cov_7.394373, whole genome shotgun sequence.
ACCESSION   DAOPMW010000450 DAOPMW010000000
VERSION     DAOPMW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61793_length_2223_cov_7.394373"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLU11_14900"
     CDS             complement(<1..756)
                     /locus_tag="PLU11_14900"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPN60474.1"
                     /translation="MKGKRIFVVGSSNTDMVVKSAKLPRPGETIMGGMFLMNAGGKGA
                     NQAVAAARLGGEVTFVAKVGNDIFGAQSVEGFKKEGIQTQYVLVDDSHPSGTALIMVN
                     EEGENCIVVAPGANAALRPADLERVEELKEAAIILMQLEIPLETIEAAKRIAVGNHRR
                     VMINPAPAQALSDELLTGLFLITPNESEAELLTGIAVTDMESATRSADFFLNKGVENV
                     VITMGKQGAYFKNGSTSFMVPAPAVKAVDTTAAG"
     gene            complement(763..>2223)
                     /locus_tag="PLU11_14905"
     CDS             complement(763..>2223)
                     /locus_tag="PLU11_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPN60475.1"
                     /translation="YMWKFGAGGDRPRKIEKLVSELIGEEKASRFWTLFRKHYITEAD
                     IKRIAELGFNSVRPALNSRLFMTEGDNPVYVEEGFQLLDSLISWCRKYNLYVIIDMHG
                     APGGQTGQNIDDSPNDLPELFMEKKYQDQLEGLWIKLATKYKDEPVVAAYDLLNEPLP
                     VGTGAAAKYKHLLVPLYKRLTAAIRKVDTRHMITLEGFDWSNDWSLFDKPFDENVFYQ
                     FHYYCWARPDLLGGIDKFIKKRAELNTPIWVGETGEKGNTIYWSTTQLFEANNIGFSF
                     WPWKKMDTENTPYSIKKPANWDLIAEYSRGGTKPDPAICIRAFDELLENIKLTNCVYY
                     QDVCNAILTRIPGKIEAENYGHTGYMQSYFVRDTTARSAYYRKTEPVKIALDSKDEKQ
                     FWSEQSVEVKKSEWLNYTFASEDGNKRKLTIRASAVNGPVLLELRMNKRKLKVMVSDT
                     GFTEIDLGNVPVKQVGNTIRVLVKSGVVKIDWLKLQ"
BASE COUNT          538 a          614 c          429 g          642 t
ORIGIN      
        1 acccgcagcc gtggtatcca cggctttgac tgcaggcgca ggtaccataa atgaagttga
       61 cccgttttta aaataagccc cctgcttccc catagttatg accacatttt ctactccttt
      121 gttcagaaaa aaatctgccg accgcgtagc agattccata tctgtcacgg caataccagt
      181 aagtaattcc gcctctgatt cgttgggtgt aatcaggaat aatccggtca gcagttcatc
      241 ggagagtgcc tgggcaggtg ccggattaat catcactcgt cgatgattgc caacggcgat
      301 tcttttagcc gcttcaatcg tctccagcgg aatttccagc tgcatcagaa ttattgctgc
      361 ctctttcagt tcttctacac gctccaggtc tgcgggccgt aaggccgcat ttgcaccagg
      421 agccactaca atacaatttt ccccttcctc attgaccata atcagtgcgg tacccgatgg
      481 atggctatca tccaccaaca catactgggt ctgaattcct tcctttttaa atccctccac
      541 gctttgggca ccgaaaatat cgttccccac ttttgcaacg aatgtaacct cccctcccag
      601 cctggcagcc gccaccgcct ggttggctcc tttgcctccg gcattcatta aaaacatccc
      661 acccataata gtttcacccg gcctgggtag tttagcggat tttaccacca tatcggtgtt
      721 cgaactgcca acaacaaata tgcgtttccc tttcattctt tcttattgaa gtttcagcca
      781 atctatcttc acaactcctg atttcaccaa caccctgatc gtattcccaa cctgcttaac
      841 cggaacattt cccaaatcga tttctgtaaa tccggtatca ctcaccataa ctttcagttt
      901 ccgtttattc atacgaagtt ccagcagcac cggcccattg accgctgacg ccctgatcgt
      961 cagtttccgc ttgttgccat cctcactggc gaaagtatag ttcagccatt ctgatttttt
     1021 tacttccact gattgttcgg accagaattg cttttcatcc ttactgtcca gcgcgatctt
     1081 taccggttct gttttccggt aataagcaga cctcgccgtg gtatccctca cgaaatagga
     1141 ttgcatatat ccagtatgcc catagttctc tgcttctatc ttacctggaa tgcgggtgag
     1201 gatcgcatta caaacatctt gataatagac acaattcgtc agtttgatat tctccaataa
     1261 ttcatcaaat gcccggatgc aaatagccgg atccggtttt gtaccccctc ttgaatactc
     1321 cgcaatcaag tcccagttgg ctggtttctt aatagaataa ggggtgttct ccgtatccat
     1381 tttcttccag ggccagaaag agaaaccgat attgtttgct tcaaataact gggtagtact
     1441 ccaatagatt gtattgcctt tttccccggt ctctcctacc cagatggggg tattgagttc
     1501 cgctcttttt ttaataaact tatcgatgcc tcccaagaga tcaggccttg cccaacagta
     1561 gtaatggaac tggtaaaata cattttcatc aaagggctta tcaaacaggg accagtcgtt
     1621 tgaccagtca aagccctcca atgtaatcat gtgccgggtg tccactttac ggatcgccgc
     1681 ggttaaccgt ttgtataagg gcaccagtaa atgtttgtac ttcgcagccg caccggttcc
     1741 tacaggtaac ggctcattaa gcaaatcgta ggccgccacc accggctcat ccttatattt
     1801 cgtggccaat tttatccaga ggccttccaa ttgatcctgg tatttttttt ccatgaacag
     1861 ctcagggagg tcgttgggac tatcatctat attctgcccg gtctgcccac cgggtgcccc
     1921 atgcatatct ataatcacat agagattata cttccggcac cagctgatca atgaatccaa
     1981 taactgaaag ccttcttcca cataaaccgg gttatctccc tcggtcatga agagccggga
     2041 attgagtgca gggcgtacag aattgaaacc cagctcagca atacgtttga tatcagcttc
     2101 ggtaatataa tgtttacgga ataatgtcca gaaacggctg gctttctctt ccccaatcag
     2161 ctccgaaacg agcttttcaa tttttcgtgg ccggtcgccc ccggcaccaa acttccacat
     2221 ata
//
LOCUS       DAOPMW010000451         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_62748_length_2200_cov_4.509557, whole genome shotgun sequence.
ACCESSION   DAOPMW010000451 DAOPMW010000000
VERSION     DAOPMW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62748_length_2200_cov_4.509557"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..997
                     /locus_tag="PLU11_14910"
     CDS             242..997
                     /locus_tag="PLU11_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN60476.1"
                     /translation="MSQFSGKVAIVTGGSRDIGRQVCLKLAAGGARVCVNYFGNKELA
                     DETLKQINAAGGEAIIAYGDMTKAADVQQVVESCVAAFGNTIHILVNVAGGLMGRKVL
                     ADLDENFWDAVIDVNLKTAFLTTRAVVPYMTDGGTIVNFSSQAARDGGGFGALAYATA
                     KGGVLTMTRGLAKELGPKGIRVNCVSPGMINTTFHNTFTKPEVRTNVAAATPLRREGE
                     ASEVADLVCYLAGPASSFINGESVEINGGTYFV"
     gene            1036..>2200
                     /locus_tag="PLU11_14915"
     CDS             1036..>2200
                     /locus_tag="PLU11_14915"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPN60477.1"
                     /translation="MRLLKTFCLLFFGFPLLTEAQHPVTFFTKTEAALVKKDLLKYPL
                     LSRSYRDIKQEVDQWLLKDIDVPVPKDPAGGYTHDKHKYNYTLMFNSGILYNLTGEKK
                     YAQLVSRTLLKYAALNPTLKNHPQATSSSPGRLFWQALNDANWLVYSGMAYDLAYNGI
                     PAADRKIIEEGAFKPEVEFITRDLQTWFDLIHNHGVWACAGVGLVGIATGNKDYIDMA
                     LYGTKKDGKSGFIALMDGLFSPDGFYAEGPYYTRYAILPYYLLATALDKVRPELKIFK
                     YRENILQKALMAGLQQTNLDGTFYSFNDALKEKDYTSNELVTAVDIAWDKYGKDDGLL
                     VVARAQNRVLLNKGGVSIAAALASGKNNTLRFPYKTVEYRDGSKGDRGGVTVLR"
BASE COUNT          604 a          465 c          549 g          582 t
ORIGIN      
        1 ccctttattt agaaaatatg taaaaaatag gataaatatc ttatttaaat taaataaagt
       61 acttttaatt aaaaatattc ttatacacaa aaaaaagctc gcctgattat tcctcttaat
      121 attaccatcg aaatcaaacc ggtaagctga aacagccata tgaaatcagg tactgcttct
      181 ctgccgtgct cgtttttagt cctgccttct ccatttatta gtagtacgta aaatattttt
      241 tatgagtcaa ttttcaggta aggtcgctat tgttacaggc ggttccaggg atatcggacg
      301 tcaggtttgt ttaaaactgg ccgctggtgg ggcacgtgta tgtgtcaatt attttggcaa
      361 taaagaactc gctgacgaaa ccctgaaaca aatcaacgcg gcaggtggcg aagcgatcat
      421 cgcttatgga gatatgacga aagctgccga tgtacaacaa gttgtagagt cctgtgtagc
      481 cgcctttgga aacacgattc atatattggt aaatgtggcc gggggactga tggggcgtaa
      541 agtactggcc gatctggatg aaaacttctg ggacgcggtg attgatgtaa atctgaagac
      601 agccttcctg acgaccaggg ccgtggtacc gtatatgaca gacggcggaa ccatcgttaa
      661 tttctcatcc caggcagcgc gggatggcgg tggatttggt gcattagcat atgcgactgc
      721 caaaggcggt gtattgacca tgacccgtgg attggccaag gaactcggtc ccaaaggtat
      781 ccgggtgaac tgtgtatctc ccggtatgat caataccact ttccataaca cgtttaccaa
      841 accggaagtc agaactaatg tggccgctgc cacaccgttg cgccgggaag gggaagcgag
      901 tgaagtagct gacctggttt gttacctggc cggacctgct tcttctttta ttaacgggga
      961 aagtgtggag attaatggcg gaacctattt tgtatagtaa tgatttgtta gcggatcttt
     1021 caatagatta attacatgcg attgctgaaa acgttttgcc tgcttttttt tggcttccct
     1081 ttactgacgg aagcccagca cccggttact ttttttacaa agacggaagc ggccctggtg
     1141 aaaaaagacc tgctgaaata ccccttgctg agccggtcct accgcgatat caaacaggaa
     1201 gtggaccagt ggctcctgaa ggatatcgat gttccggttc ccaaagatcc ggcgggaggg
     1261 tatacccatg ataaacataa gtacaattat accctgatgt ttaacagcgg tatattatat
     1321 aatctgaccg gcgaaaagaa gtatgcacaa ctggtcagcc gtactttatt gaagtatgca
     1381 gccctgaatc ccacgttgaa aaatcatccg caagccacca gcagttcacc cggtcgcctg
     1441 ttctggcagg cactcaatga tgcaaactgg cttgtatata gcggcatggc gtatgacctg
     1501 gcctataacg gtatccccgc agccgatcgt aaaataatag aagagggtgc ttttaaaccc
     1561 gaagtggaat tcattacccg tgatctccag acctggtttg atcttattca taatcatggt
     1621 gtctgggcct gcgcaggtgt gggactggtt ggaatcgcca ccggtaataa ggactatatt
     1681 gatatggcct tatatggcac aaagaaagat gggaaaagtg gctttatcgc cctgatggat
     1741 ggtttgtttt caccggacgg cttttatgcg gaaggccctt attatacacg ttatgcgatc
     1801 ctgccttatt atttattggc cactgcattg gacaaagtac gtcctgaact gaagatcttt
     1861 aaataccggg agaatatttt acagaaagca ttgatggcgg gtctgcaaca aacaaacctg
     1921 gatggcacgt tctactcatt caacgatgcc ctgaaagaaa aagattatac gtccaatgaa
     1981 ctggtgacgg cggtggatat tgcctgggac aaatatggca aggatgacgg tctgctggtg
     2041 gtggcccgtg cacaaaaccg ggtgctgctc aataaaggcg gggtatccat tgctgccgcg
     2101 ttggcatccg gaaaaaacaa tacgctccgg tttccttata aaacagtgga ataccgggat
     2161 ggcagtaaag gggacagggg cggtgtaacc gtattacgca
//
LOCUS       DAOPMW010000452         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_63421_length_2184_cov_7.473462, whole genome shotgun sequence.
ACCESSION   DAOPMW010000452 DAOPMW010000000
VERSION     DAOPMW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63421_length_2184_cov_7.473462"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLU11_14920"
     CDS             <1..434
                     /locus_tag="PLU11_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN60478.1"
                     /translation="LVSFIELVQQDKFRGESTVKTFLYSLNRYTWLNELKRRGRALAR
                     EEKYEKGQVHIEMDAGHTIANREGKAEVLKVVAELGDTCKKILLLFYYENLSMKEILD
                     QTDYESEQVVRNKKYKCLKQLEQMVNANPALRETLKNLIHG"
     gene            427..1191
                     /locus_tag="PLU11_14925"
     CDS             427..1191
                     /locus_tag="PLU11_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60479.1"
                     /translation="MDNSTPEMDEKLVRLLDGDLSPEERITVEQQLAGNALLLQQYDR
                     LLATKAAIRHFGLQQQVGAIHSQMMAELNAPIRKISPVRKMIRYTSAIAAGILLLVAG
                     FFAYQYFSLTPEKVFSANYQAYELNTSRGTTTNSLIEEAFRQKKYEEVIRLRDAATEP
                     NIKNEFLAGAAALELNNLSLARQRFASVIEQNKTANTTVLNDEAEYYLALTQVRQKDF
                     AAAVMLLEKIQQDSGHTYSAKVKNGLLNDVRKLSDQ"
     gene            1370..>2184
                     /locus_tag="PLU11_14930"
     CDS             1370..>2184
                     /locus_tag="PLU11_14930"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HPN60480.1"
                     /translation="MKSKFLITAIALFSASLVFYSCQKEVSGNSTDPNKPLPVSIYLT
                     DHQTPVFDSVFIDIQGLQVKLEDDTLPNGGWVTLPIRPGVYNILRFRNGLDTLFGNGT
                     LPNARLKKVKLVLGTQNSVMKDGQSTPLRIKDEDREVIANLDASNFEISNGQVLFWID
                     FDAGNSIQVDNSGSGNNNGYRLKSHIKIFTRSRSGSIEGRILPRAADAIVKAVIGSDT
                     ATAIPFDDNGEFKIVGLNAGSYTLLIDGQNGYRDTAINNVIVRNREDTHIPVIT"
BASE COUNT          647 a          487 c          507 g          543 t
ORIGIN      
        1 tgctggtcag ttttattgaa ctggtacaac aggataaatt caggggagaa tccactgtga
       61 agactttttt atactcattg aaccggtaca cctggctcaa tgaactgaaa agaaggggca
      121 gggccctggc cagggaagaa aagtatgaga aaggccaggt gcatatagaa atggatgccg
      181 gtcataccat cgccaaccgg gaagggaagg cggaagtgct gaaagtggtg gcggaactgg
      241 gcgatacctg taaaaagatt ttattgcttt tttattatga aaatctctcg atgaaagaaa
      301 tcctggatca gaccgattat gaatctgaac aggtggtgcg gaataaaaaa tataaatgtc
      361 tgaaacagct ggaacaaatg gtgaatgcca atccggcttt acgagaaacc ttaaaaaacc
      421 tgatacatgg ataattctac acctgaaatg gatgaaaaac tggttcgcct gctcgacgga
      481 gacctttctc cggaagaacg gataaccgtg gaacaacaac tggccggcaa tgccctgctg
      541 ctacagcagt atgacagact gctggctaca aaagcggcta tccgtcattt cgggttgcaa
      601 cagcaggtgg gggctattca tagtcagatg atggccgagt tgaatgcacc aataagaaaa
      661 ataagtccgg ttcgtaaaat gatccggtat acttcggcta ttgcggcggg tatcctgtta
      721 ttggttgccg gtttttttgc ctatcagtat ttcagtctta ctccggaaaa agtattcagt
      781 gccaattacc aggcgtatga acttaatacc agccgtggta ccactaccaa ttctttaatt
      841 gaagaagctt ttcgtcaaaa aaaatatgaa gaggtgattc gtttacggga tgctgccaca
      901 gaaccgaata ttaaaaatga gttcctggca ggagccgccg ccctcgaact gaataacctt
      961 tccctcgcca ggcaacggtt tgccagtgtg atcgaacaga ataaaacggc caacacgacc
     1021 gtgctaaatg acgaggctga gtattacctg gcattgaccc aagtccgtca gaaggatttt
     1081 gccgccgccg taatgttact tgaaaaaatt cagcaagact cagggcatac ctattctgct
     1141 aaagtaaaga acggtctgct taacgatgta cgcaaactca gtgatcaata aacctgatta
     1201 ttagtagcac taagcctctt ccttgcggag aggttttttt ttgcccttaa ggttacatcc
     1261 ggacttccgg tgttgactat ttatggtaag caggggtgcc tgttaaatga ataacctgct
     1321 aaacagtccg tattttctaa acagtaacaa acaacaaaaa aacacaacca tgaaaagcaa
     1381 gtttttaatc acggccattg ccctcttcag tgcttccctg gtattttatt cctgccagaa
     1441 agaagtatcc ggtaacagta ctgaccccaa caaacccctg ccggtatcca tttacctgac
     1501 ggatcatcag acaccggtgt tcgacagtgt gttcattgac atccagggct tacaggtaaa
     1561 actggaagat gataccttgc ccaatggcgg ctgggttaca cttcctatcc gtcccggggt
     1621 ctataatatc ctccgtttcc ggaatggatt ggatacctta tttggcaacg gtacacttcc
     1681 caatgcccgc ttaaagaaag tgaagctggt actcggtaca cagaacagtg tgatgaagga
     1741 tggacagtca accccgctcc gcatcaaaga tgaagatcgt gaggtgattg ccaacctgga
     1801 tgccagcaat tttgaaatca gcaacggaca ggtattattc tggatcgatt ttgatgcagg
     1861 aaattccatc caggtggaca acagtggttc cggtaacaat aatggctatc gcctgaaatc
     1921 ccatatcaag atctttacgc gcagccgttc cggcagcatc gaaggaagaa tcctgccccg
     1981 cgctgcagac gccattgtga aagctgtcat cggatccgat accgccaccg ctattccctt
     2041 tgatgataat ggagaattta aaattgttgg actcaatgcc ggttcttata ctttattaat
     2101 cgatggacaa aacggttaca gggataccgc catcaataat gtaatcgtcc gcaacaggga
     2161 ggatacacat atcccagtga tcac
//
LOCUS       DAOPMW010000453         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_63762_length_2176_cov_8.723715, whole genome shotgun sequence.
ACCESSION   DAOPMW010000453 DAOPMW010000000
VERSION     DAOPMW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63762_length_2176_cov_8.723715"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="PLU11_14935"
     CDS             complement(<1..2081)
                     /locus_tag="PLU11_14935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPN60481.1"
                     /translation="MKRIVFFDIELNPQTETIEDIGCIQDDGAVFHKKDIPGFFDFIK
                     DAEFICGHNILAHDINWLQKHTSEKDWGKERAIDTLLFSPLLFPKKPYHSLLKDDKIL
                     SEERNNPVNDSKKAKELFDEEVTAFNELPAELKLLYYNLLNGRPGFNSFFVYIGYNTV
                     AEEEGISRIIKRFFHHQVCESADIAQLIKAYPVELAYALSLIHCNDRYSITPPWVLKK
                     YPKVEQVLYLLRSNPCLTGCAYCEQAFNPYRVLKSYFGYDGFRKYDDEPLQEKAVMSA
                     VNNDSLLAVFPTGGGKSITFQVPALMAGENAKALTVVISPLQSLMKDQVDNLEKKGIT
                     DAVTINGLLDPIERSKAVERVEDGSAFILYISPESLRSVTIQRLLLKRKIARFVIDEA
                     HCFSSWGQDFRVDYLYIGDFIKNLQDIKGPDTRIPVSCFTATAKQKVIEDIQQYFKEK
                     LGLNLVLYRATAGRKNLQYEVFKKDTDEEKYAQLRDLIETRNCPTIVYVSRTQRAYKL
                     ATRLTEDGLEAKAYHGKMAKEEKTANQNAFMGGNVNIMVATSAFGMGVDKDNVGLVIH
                     YDISDSLENYVQEAGRAGRDEKIKAECQILFSEDDLDKHFILLNQTKITVKEINQIWK
                     AVKDLTKVKDSVSNSALEIARRAGWDESVKEIETRVVTAIAALEGAGYIKRGQNMPRV
                     YANSILSANAQ"
BASE COUNT          553 a          510 c          444 g          669 t
ORIGIN      
        1 tcctgggcat tcgcgctgag tatactgttg gcatacactc ggggcatgtt ttgtcccctt
       61 ttgatatagc ctgccccctc caaagccgca atcgcggtca ccaccctcgt ttcaatttcc
      121 tttacgctct catcccaacc cgcccgccgg gcaatctcca gtgccgaatt ggatacactg
      181 tcttttactt tggttagatc ctttacggct ttccagatct ggttgatttc ctttaccgtg
      241 atcttggtct ggttgagcag gataaaatgt ttatccaggt catcctctga aaacagaatc
      301 tggcactctg ctttaatttt ttcatcccgt ccggcacgtc ctgcctcttg cacatagttc
      361 tcgagtgaat cagatatatc ataatggata actagtccta cgttgtcttt gtccaccccc
      421 attccaaatg ccgatgtagc caccatgata ttcacattcc cgcccataaa agcattctga
      481 ttggccgttt tttcttcttt agccattttt ccatgatatg cttttgcctc aagtccatct
      541 tccgtcagtc tggtagccag tttgtatgcc ctttgtgtac gagacacata gacgatggtt
      601 ggacaattgc gggtttcaat aaggtctctt agttgcgcat atttttcttc gtccgtatct
      661 tttttaaaca cctcatactg caggtttttc cgtcctgcag tcgctctgta taatacaaga
      721 tttaatccca gtttttcctt gaaatactgc tgaatgtctt caattacttt ttgtttggcg
      781 gtagcggtaa aacaggaaac gggtattctt gtgtccggcc cttttatatc ctgcaggttt
      841 ttaataaaat ctccgatata gagatagtct acccgaaagt cctgacccca ggaagaaaag
      901 cagtgggcct catcaatgac aaaccgggcg atttttcttt ttagcagtag tcgttgaata
      961 gtaacagaac gaagggattc gggtgaaatg tataagataa aagccgagcc atcctccacc
     1021 cgttcaactg ccttgcttcg ttcaatggga tccagcagcc cgttaatggt tactgcatcg
     1081 gtaatcccct ttttctccag gttgtccacc tgatctttca tcagtgattg taaaggagaa
     1141 atgaccacag tgagtgcctt ggcattttca ccagccatca gtgcgggtac ctggaaagtg
     1201 atagacttcc cgccaccagt tgggaaaacc gccagtaagg aatcattatt aaccgcagac
     1261 attacggctt tctcctgcaa tggctcatca tcgtatttcc ggaatccatc atagccaaaa
     1321 taacttttca gcacccggta gggattaaaa gcctgttcac aataggcgca tccggtaaga
     1381 cagggattgc tccgcaacaa gtacaatacc tgttcaactt tgggatactt tttcagtacc
     1441 cagggaggag taatggaata gcggtcgtta caatgaatta gtgatagggc ataggccagt
     1501 tcaaccggat aggcctttat cagttgtgca atatccgcgg attcacaaac ctgatgatga
     1561 aaaaagcgtt ttatgattcg tgatattcct tcttcttctg ccacagtatt atatccgata
     1621 tacacaaaga aggaattaaa ccccggcctt ccattcagca aattataata cagtaatttg
     1681 agttcggcag gcaattcatt aaaagcggtt acttcctcgt caaataactc tttcgctttt
     1741 tttgaatcgt tgaccgggtt gtttcgctct tctgaaagaa tcttatcatc ttttaagagg
     1801 ctgtggtagg gtttctttgg aaacaacaaa ggagaaaaca acaaggtatc aatcgcgcgt
     1861 tctttccccc agtctttttc agatgtatgc ttttgaagcc agttgatgtc atgcgccagg
     1921 atattatgtc cgcagatgaa ttcagcatct tttataaagt caaagaatcc cggaatatcc
     1981 tttttgtgaa acacagctcc atcatcttgg atgcagccga tatcttcaat cgtttccgtt
     2041 tgggggttga gttcgatatc aaaaaagacg atccttttca tgattaacta ttttgaaaag
     2101 ggtttgtacc aatctggaga caaaacgtgg aatcccggtt taaacagcag ggctgatctc
     2161 cggcggcatg agtaaa
//
LOCUS       DAOPMW010000454         2164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_64307_length_2164_cov_9.013751, whole genome shotgun sequence.
ACCESSION   DAOPMW010000454 DAOPMW010000000
VERSION     DAOPMW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64307_length_2164_cov_9.013751"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..722)
                     /locus_tag="PLU11_14940"
     CDS             complement(81..722)
                     /locus_tag="PLU11_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN60482.1"
                     /translation="MKKVYFISGLGADKRIFSFLDLSFCEPVFIDWITPLKNEKLESY
                     ALRLRQQIPEESPWIVGISFGGMLTVEIAKADPAVHAIILSSNKTKKEFPVYFRAGLY
                     LPLYKWSPARLSKKFTLRTSSLLGGTNEAEKKLLHQIIRESDIHFVRWAISAILHWKN
                     MTVPENIIHIHGTADKLLPSRYVKADYLIAGGTHVMTLDKHPEISQLLQQLIQ"
     gene            complement(731..2008)
                     /gene="tyrS"
                     /locus_tag="PLU11_14945"
     CDS             complement(731..2008)
                     /gene="tyrS"
                     /locus_tag="PLU11_14945"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPN60483.1"
                     /translation="MNLIEELRWRGMIADIMPGTEEQLNKEMTTGYIGFDPTADSLHI
                     GSLVPILLLVHFQKAGHKPIALVGGATGMVGDPSGKSEERNLLSEEALQKNVAGVKGQ
                     LEKFLDFNPALPNAAEMANNYDWFKGISFIDFLRDAGKHITVNYMMAKDSVKKRIEGE
                     TGISYTEFAYQLMQGYDFYWLHQHKNCKLQMGGSDQWGNMTTGTELIRRKSGGEAFVF
                     TNPLITKADGGKFGKTESGNVWLDPVRTTPYQFYQFWLNASDTDAEKWIRIFTFLTRD
                     TIDMLIHEHQMNPSARVLQKTLAQEITTFVHGKEEYEKAVETTQKLFANQNAPAESLS
                     TEDLEGMEGVIKVDFAKDKIVAGTDIVSFLAETGILSSKGEARKLVEGGGISINRKKA
                     DGVKMTIDSSLLLHDKYILVQKGRKNYYLVQAV"
BASE COUNT          507 a          517 c          488 g          652 t
ORIGIN      
        1 aaatcctgac ctacgaccgg gtccacatga atgcaaaagg gaaccagttt ctcgccgacg
       61 cgatgtggac taccataaaa tcactgaatt aattgttgta acagctggct gatttcgggg
      121 tgtttatcca gtgtcatcac atgggtgcca cccgcaatta aataatctgc ttttacatac
      181 cgggagggaa ggagtttgtc tgccgttcca tgtatgtgaa taatattttc cggaacagtc
      241 atattcttcc aatgcagaat ggcgctgatg gcccagcgta caaaatggat atcactttcc
      301 cggatgatct ggtgcagcag ttttttctct gcttcattcg ttccgcccaa tagggagctt
      361 gtacgtaatg tgaatttttt agacaaacgg gccgggctcc atttatagag tggcaggtaa
      421 agtcccgccc tgaaatagac cgggaattcc ttctttgttt tattgcttga aagaataatg
      481 gcgtgtactg cagggtcggc cttggctatt tccaccgtca gcattccgcc aaaggatatg
      541 cctacaatcc agggtgattc ttccggaatc tgctgacgca gccgcagcgc ataactttcc
      601 agcttttcat ttttcagcgg ggtgatccag tctataaaaa ccggttcaca gaaggacagg
      661 tcaagaaagg agaaaatcct tttgtcggcc cctaatccgc tgataaaata aacctttttc
      721 aatttattat ttaaacagcc tgtaccaggt agtaattctt tcttcctttt tgtaccagga
      781 tgtatttgtc atgaagtaac aggctgctgt caatcgtcat tttcacccca tcggcttttt
      841 tccggttgat actgatcccg ccaccctcaa ccaatttacg ggcttcccct ttactggaga
      901 gaatacctgt ttcggccagg aatgaaacga tatcggtgcc tgccacgatt ttgtcttttg
      961 caaaatccac tttgatcaca ccttccatgc cttccaggtc ttcggtgctc aggctttcgg
     1021 ccggtgcgtt ttgattggca aaaagttttt gggtggtttc cacggctttt tcatactctt
     1081 cttttccatg tacaaacgtg gtaatttcct gcgccagtgt tttttgaagt acccgggcgg
     1141 aaggattcat ttggtgttca tggatgagca tatcaatggt atcccgggta aggaaagtga
     1201 agatgcggat ccatttttcc gcatcggtat cactggcatt gagccagaac tgatagaact
     1261 ggtagggagt agtgcgtacc ggatccagcc atacattccc gctttctgtt ttaccaaatt
     1321 tgccgccatc ggctttcgtg atcaggggat tggtaaaaac aaaagcctcc ccaccgcttt
     1381 tacggcggat cagttcggtg ccggtggtca tattacccca ctggtcgctg ccacccatct
     1441 gcagtttgca atttttatgc tggtgcagcc agtagaaatc atagccctgc atcagttggt
     1501 aagcaaattc cgtatagctg atgcccgttt cgccctcaat tctcttcttc acactatcct
     1561 tggccatcat ataattcacc gtgatatgtt tgcccgcatc ccgcaggaaa tctataaagg
     1621 agatgccttt gaaccagtca tagttattgg ccatttctgc tgcgttgggc agggccgggt
     1681 tgaagtcaag gaatttttcc agctggcctt ttacgcccgc cacatttttt tgtaaggctt
     1741 cttcgctgag caggtttctt tcttcacttt ttccgctggg gtcccccacc attccggtgg
     1801 ctccgccaac gagggcaatg ggcttgtggc cggctttctg aaaatgaacg agcagtaaaa
     1861 tcgggaccag gctaccgata tgcagactgt cggctgttgg gtcaaagccg atatatccgg
     1921 tggtcatttc tttgtttaat tgttcctccg taccgggcat tatatccgca atcatgcctc
     1981 tccagcgtaa ttcttcgatc aggttcattt tttaattaat tgattgtcaa tgtgggggga
     2041 tatgtaatta cccctaataa tccagcaaaa ataaggcaga atgcagattt aggccctgcc
     2101 ggggcaatat tcatgtaaaa tattcgtttt caatgattag tctaaaccct ccacaatgtt
     2161 atga
//
LOCUS       DAOPMW010000455         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_70152_length_2041_cov_6.784491, whole genome shotgun sequence.
ACCESSION   DAOPMW010000455 DAOPMW010000000
VERSION     DAOPMW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70152_length_2041_cov_6.784491"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="rsgA"
                     /locus_tag="PLU11_14950"
     CDS             complement(<1..629)
                     /gene="rsgA"
                     /locus_tag="PLU11_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPN60484.1"
                     /translation="MKAIVYKSTGSWYTLKDEGGRFWNGRMKGVMKLDDITSTNPVAV
                     GDWVEADIENDDSNTAIINEIHDRNNYINRQSPRHKHQHHIVAANLDQSLLIATLREP
                     RTSQGFIDRFLVACEMYHVAAVIVFNKSDLYKAKDMEKYEEWKAMYEAIGYKVILVSA
                     EKNEGLDQFGQVLKDKTTLLSGHSGVGKSSLLNTMFPGMNRKTQDVSGW"
     gene            complement(657..1670)
                     /locus_tag="PLU11_14955"
     CDS             complement(657..1670)
                     /locus_tag="PLU11_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPN60485.1"
                     /translation="MFYEWIRHMDFGFPESFALLALLPLLIWWYVTNNNRQQARVKVS
                     TIRAFTVRSWKNRFRHLPFIFRLLAFTALIFALARPQTRNDQQQTEGEGIDIVLCMDV
                     SGSMGSRDILPSRMEVAKQVAEEFVRSRPVDRIGLVIFSGESFTQCPVTTDRNTLITQ
                     IQHLESRRYLKDGTVIGEGLATAVDRLSDSKGKSKVVILLTDGKEDPPETRLIDPLTA
                     LEIAKSKGVKVYAIGMGAAPSSIVENTGNVSYKKNPMVDFLDEELLKKMADETGGQFF
                     RAKDKEGLQDIYRQIDKLEKSKVEVQSFKRYEERFIPFLLAALFFVFLGQLLHFTLLR
                     KFP"
     gene            complement(1670..2026)
                     /locus_tag="PLU11_14960"
     CDS             complement(1670..2026)
                     /locus_tag="PLU11_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60486.1"
                     /translation="MAEVKAVPVISPLDEALDGLQKLKSANAETKLFHTRLADIFRMY
                     VYRKKGLLSLQKTTDDLVVQLRELGLSKAEFDELAQVLRLGDFVKFARFIPSATDNEK
                     AFETIKKSIEKIEQLN"
BASE COUNT          500 a          578 c          410 g          553 t
ORIGIN      
        1 ctccagccac tcacatcctg ggtcttccgg ttcatacccg gaaacatggt atttaacagg
       61 gaagatttcc cgacaccact atgtccgctg agcaaggtgg ttttatcttt cagcacctgt
      121 ccgaattgat ccaacccctc gtttttttcc gcactgacca ggattacttt atacccaatg
      181 gcttcataca tggctttcca ctcctcatac ttctccatat cctttgcctt atacagatcc
      241 gatttattaa aaacgatgac cgccgccaca tggtacattt cgcaagccac caggaaacga
      301 tcaataaagc cttgtgaagt ccggggttcc cgcagggtgg ctatcaggag cgactgatcc
      361 aggttggccg ctacaatatg atgctggtgt ttatgccggg gcgattgccg gttgatataa
      421 ttattccggt catgtatttc attgatgatc gcggtattgc tatcgtcatt ttcaatatcc
      481 gcttccaccc agtcacctac cgccaccggg ttagtgctgg tgatatcatc cagtttcatc
      541 acgcccttca tacggccatt ccagaaacgg cccccctcat ctttgagtgt gtaccagctg
      601 cccgtggatt tataaacaat tgctttcatg cgcctgcaat atactcgata aaaagatcag
      661 gggaatttgc ggagcagggt aaaatggagc agttgcccga ggaagacaaa aaacaaagcg
      721 gccagcagga agggaataaa ccgctcttca tatcttttaa acgactgaac ctctactttt
      781 gatttttcca gtttatcaat ctgccggtaa atatcctgta acccctcttt atcttttgcc
      841 ctgaaaaact gccccccggt ttcatccgcc attttcttca gcaactcctc atccaggaaa
      901 tcaaccatcg gattcttttt ataagaaaca ttcccggtat tctcgacaat actggaaggc
      961 gccgctccca tgccaatggc atacactttt acccctttcg acttggcgat ctccaaagct
     1021 gtcagcggat caatcagccg ggtttcaggc ggatcttcct tcccatcggt cagcaggatc
     1081 accaccttgc ttttcccttt actgtccgac aaccgatcca ccgctgtggc cagtccctcg
     1141 ccaatcaccg ttccgtcttt cagatacctc cggctctcca ggtgctgaat ctgggtaatc
     1201 aatgtattcc ggtctgtagt caccgggcac tgcgtaaaac tttccccgga aaaaataacc
     1261 agtccgatcc ggtctaccgg gcgactgcgt acaaattcct ccgccacttg tttggccact
     1321 tccatccggg aaggaaggat atcccgggag cccatactac cgctgacgtc catgcataac
     1381 acgatatcaa ttccctctcc ttcggtctgc tgctggtcat tccttgtttg cggacgggcc
     1441 agtgcaaaga tcagggccgt aaatgccagt aaccggaaga taaaaggcag atgcctgaac
     1501 cggttcttcc aggaacggac cgtaaaagcc cggatcgttg acacttttac ccttgcctgt
     1561 tgccggttat tatttgtaac ataccaccat atcagcaatg gcagtaaggc caggagcgca
     1621 aacgactcgg gaaagccaaa atccatatgt ctgatccatt cgtaaaacat cagttcagtt
     1681 gttcaatctt ttctattgat tttttaatcg tttcaaaagc tttctcatta tccgttgcag
     1741 acggaataaa ccgggcaaat tttacaaaat cacccagccg taatacctgg gccagttcat
     1801 caaactccgc tttacttaac cccagttccc ttaactgtac taccaggtcg tccgtggtct
     1861 tctgcaagga tagcaggcct tttttcctat acacatacat ccggaaaata tccgccagcc
     1921 gggtatgaaa caacttggtc tccgcattgg ccgacttaag tttctgtaaa ccatccaacg
     1981 cttcatcaag cggtgagata accggtaccg cttttacttc cgccactgcc ggtttcaact
     2041 a
//
LOCUS       DAOPMW010000456         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_72423_length_1997_cov_5.315139, whole genome shotgun sequence.
ACCESSION   DAOPMW010000456 DAOPMW010000000
VERSION     DAOPMW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72423_length_1997_cov_5.315139"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..877)
                     /locus_tag="PLU11_14965"
     CDS             complement(68..877)
                     /locus_tag="PLU11_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPN60487.1"
                     /translation="MALVTLTSDIGSPDYLVGAIKAQLLQTNPEFRLIDISHTIPPFN
                     YPQAAYVCRSAIRQFPDFSYHIILVNLFDKKPEQLLLAFHQNQYFLCADNGLLTMILE
                     DNPEVIIGLPLDKEAVKNTIYLTSVMGEAVNKLVRGESIQGIGIPDVPYQEKRHLRPM
                     VENNSIEGQIIFIDSFENVIVNITREQFEEQRMGRKFTIVFKRDEVIDKISETYADVP
                     EGEKLALFNSAGYLEIAINKGNAAGLFGLKGFSEKLRQAQLSYQTVRVYFS"
     gene            complement(907..1716)
                     /locus_tag="PLU11_14970"
     CDS             complement(907..1716)
                     /locus_tag="PLU11_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN60488.1"
                     /translation="MSTYYKDKVVVVTGGTDGIGKALVEKLLDLGAKVATCGRSHDKL
                     YQLQASHPSSALHTMVADVSNENDCRRLMETTIKMFGGIDVLINNAGISMRAILQEAN
                     IDVFKKVMDINYFGVIYCTKYALDSIIERKGTIVGVSSIAGYRGLPGRSGYSASKFAL
                     QGWLESIKTELMTQGVHVMWVCPGFTTSNIRNAALNKDADSHGETPMDESKMMPAGEC
                     ADHILRAIRKKKRTLVLTFTGKRTVFMQKFLPGLADKMVHKFFFKDGKLAK"
BASE COUNT          509 a          449 c          482 g          557 t
ORIGIN      
        1 cggttccgct tccgcagaaa tagcaattta ggaaaacttt cgtacttctg gaagttccgg
       61 ggaattttta agaaaagtaa acacggacag tctgatagga gagctgggcc tgtcttaatt
      121 tttccgaaaa gcctttcaga ccgaataaac cggccgcgtt gcctttgttg atagcgattt
      181 cgaggtagcc ggcactgttg aaaagggcca gtttctctcc ttccggcaca tcggcatagg
      241 tttcactgat tttatcaatc acttcatccc gtttgaacac gatggtgaac ttcctgccca
      301 tacgttgttc ttcaaactgc tcacgggtaa tattgacaat cacattttca aaactgtcaa
      361 taaatatgat ttgcccttca atactgttgt tttccaccat gggccgcagg tgtctttttt
      421 cctggtaggg cacatcaggt atcccgatgc cctggatgga ttcgcccctg accagtttat
      481 tgaccgcttc gcccatcact gaggtaagat agatggtgtt tttaaccgcc tccttatcca
      541 gcgggagtcc gatgattact tccggattgt cttccaggat catcgtgagc aacccgttat
      601 cggcgcaaag aaaatactgg ttctggtgaa aagccagtaa gagctgttcc ggctttttat
      661 caaacaggtt gaccaggatg atatgataac tgaaatccgg gaattgccgg atcgcactcc
      721 ggcagacata ggcagcctgc gggtaattga aaggcggaat cgtatgcgaa atatcgatga
      781 gtctgaactc cgggttggtc tgcaatagct gcgccttaat tgctcccacg agataatcgg
      841 gactgccaat atctgatgta agggttacta aggccaaaac gaaaagggtt taaagggtca
      901 gcgggattat ttggccagtt tgccgtcttt gaaaaagaat ttatgaacca tcttgtccgc
      961 cagcccgggc agaaatttct gcataaaaac tgttcgtttc ccggtaaagg tcagtaccag
     1021 tgtccttttc tttttccgga tggcccgcag aatatggtcg gcacattcac cagccggcat
     1081 cattttgctt tcatccatcg gggtttcgcc gtgcgaatcc gcatctttat tcagggcggc
     1141 gttgcggata tttgaggtag tgaaaccagg acaaacccac atcacatgca ccccctgggt
     1201 cattaactcc gtcttgatcg attccagcca gccctgaagg gcaaatttac tggctgaata
     1261 accgctacgg ccagggaggc cgcggtaacc ggcaatggaa gagacaccga caatagtccc
     1321 ttttcgttct ataattgaat cgagggcata cttggtgcaa tagatcaccc cgaagtaatt
     1381 aatgtccatt acttttttga aaacatcaat attggcttcc tgcaggattg cccgcatgga
     1441 aattccggca ttattgatca gcacatcaat accgccaaac attttgatgg tggtttccat
     1501 cagccggcgg caatcatttt cattgctcac atcggccacc atggtatgca gggcagagga
     1561 ggggtgactg gcttgtaact ggtacaattt atcatggctc cggccacagg tagccacttt
     1621 ggctcccaga tcaagcaatt tttctaccaa tgctttcccg atcccgtctg tacctccggt
     1681 cacgacgaca accttatctt tatagtaagt ggacatggtg agtggtttat accacaaacc
     1741 tactaaaaaa gttggaggtt ggaggttgga ggttcgaggt tggaggttgg aggttcgagg
     1801 ttggaagttg gaggttcgag gttggaagtt cgaaaatttc ataacggttg gcgacggtat
     1861 gaatttgtga aagttttttt gtagatattt agaccgatag gttcaattga cttattcctc
     1921 ttatttatga aaattcagta taatggcaac cgtttcacga tttgaagaac ttgaaatatg
     1981 gaaaatggcc agagagc
//
LOCUS       DAOPMW010000457         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_82614_length_1830_cov_7.741408, whole genome shotgun sequence.
ACCESSION   DAOPMW010000457 DAOPMW010000000
VERSION     DAOPMW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82614_length_1830_cov_7.741408"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLU11_14975"
     CDS             <1..181
                     /locus_tag="PLU11_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN60489.1"
                     /translation="GEIRVGDKVKMKKNHQVGQVIELRGKKAIVKIGMLPIQTDLAGL
                     VVVKEKVADPPADMK"
     gene            complement(231..659)
                     /locus_tag="PLU11_14980"
     CDS             complement(231..659)
                     /locus_tag="PLU11_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPN60490.1"
                     /translation="MINVATVLNRKNFKPVTVAPDTSVIEALGIMAIKNIGSLVVMRG
                     EEYLGIVTERDYSRKVILKGKHSNETRVEEIMSTDLPHIKPSDSIDHCMELMSGQNIR
                     YMPVFDNGKLTGIISMTDVVKETILAQKETINQLHSYINS"
     gene            787..>1830
                     /locus_tag="PLU11_14985"
     CDS             787..>1830
                     /locus_tag="PLU11_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN60491.1"
                     /translation="MAVVNTTVRMEQKPTTIKEIAKRLNVSVSTVSRALHNHSSIGLR
                     TKMQVQQLAAELNYEPNQAAISFKQGKTMTLGVILPNLQEEFFSLAINGIEDIASRNN
                     YTVLIGQSRDSAEREARIVDTMRRHRVDGLVVSLAKTTKNFDHFTQLEKYNIPVVFFD
                     RIPDLPDMYSVSCNLQNSSIELVDWLTAQGHRKIGFIKGPDTLLPTRERLLGYQQGLK
                     KHGIKPESSYVVSTDLSAAKTADAIHKLLALRNRPTAVITFNDYVALDAMKYVKQLGL
                     NINKDIDFVSFANLPVTSYLDHPPAASVEQFPYQQAEKATEVLLQLINKQEPPTAVRR
                     IVMEGRLVINKKQQ"
BASE COUNT          525 a          387 c          429 g          489 t
ORIGIN      
        1 aggcgagatc cgggttggag ataaagtgaa aatgaaaaaa aatcaccaag tgggacaagt
       61 gattgaatta agaggtaaaa aagccatcgt aaaaatcgga atgctgccca ttcagactga
      121 tcttgccgga ctggtggtcg tgaaagaaaa agtagcagat ccccctgcag atatgaaata
      181 aaaaaaacga agccttacgg ggcttcgttg ctatttaatg agcaggtaag tcagctgttg
      241 atgtaactgt gtaactggtt aatggtttct ttctgtgcca gtattgtttc tttcaccaca
      301 tcggtcattg atataattcc ggttaatttt ccgttgtcaa acacaggcat atagcggata
      361 ttctggcctg acatcagttc catacaatga tcaatgctgt cggacggttt gatatggggt
      421 aagtcagtgg acatgatttc ttccactctt gtttcattgg aatgttttcc tttcagaatc
      481 actttgcgtg aataatcccg ttccgtaact atgccaaggt actcttcacc ccgcataacg
      541 acgagggaac caatgttttt tatggccatg attcccaggg cctctataac agatgtatcc
      601 ggggcaaccg taaccggctt aaaatttttt ctgtttaata cagtcgcaac attaatcata
      661 tggcgtatgt ttctgatgca aaagttacaa aataaacagg tttatacaaa atcatttttt
      721 agaaggccgt aaaattacag gcccggtaat cagctaagta gtagttctca cttaatatgt
      781 tgtaacattg cggttgtaaa caccacggtc cggatggagc agaaacccac aaccataaaa
      841 gagatagcga aacgactgaa tgtctcggtt tcgaccgttt ccagggcgct tcataatcac
      901 tcgagtatcg gcttgcggac aaaaatgcag gtacaacaac tggcagcgga actcaattat
      961 gagcccaatc aggccgctat ctcattcaaa caggggaaga cgatgaccct gggcgtcata
     1021 ttgcccaacc tgcaggaaga gtttttttca ctggcaatta atgggataga agatattgcg
     1081 agccgcaata attatacggt actcatcggc cagtcaaggg atagtgccga gcgtgaagcc
     1141 cggattgtgg atacgatgcg ccgccaccgg gtggatggcc tggtggtatc cttagctaaa
     1201 accaccaaaa attttgacca ttttacccag ttggaaaaat ataatatacc ggtcgttttc
     1261 tttgaccgga ttccggatct tccggatatg tattcggttt cctgtaatct gcaaaacagt
     1321 tcaattgagc tcgtggactg gctgacagct cagggccacc ggaagatcgg gttcattaaa
     1381 ggtcctgata cattgctgcc cacccgtgaa cgcttactcg gttatcagca ggggctgaaa
     1441 aaacatggta ttaagccgga gtcttcttat gtggtatcca ctgacctcag tgcggcaaaa
     1501 accgctgatg cgatacataa actgctggct ttaaggaacc gtccgaccgc ggttatcact
     1561 tttaatgatt atgtggcgct tgatgccatg aagtatgtaa agcaacttgg gttgaatatc
     1621 aataaggata ttgattttgt aagttttgcc aacctgccgg taaccagtta cctggatcat
     1681 cctccagccg cctcggttga acagttcccg taccagcagg cggaaaaagc gacagaagta
     1741 ttgttgcagc ttattaataa gcaggaaccg cctactgcgg tgcgccggat agtgatggaa
     1801 ggacgtcttg ttataaataa aaagcagcag
//
LOCUS       DAOPMW010000458         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_88694_length_1749_cov_6.479339, whole genome shotgun sequence.
ACCESSION   DAOPMW010000458 DAOPMW010000000
VERSION     DAOPMW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88694_length_1749_cov_6.479339"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PLU11_14990"
     CDS             complement(<1..1602)
                     /locus_tag="PLU11_14990"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPN60492.1"
                     /translation="MKKTNYNKIVLLGAFIIALLAGSCKKFLDQKPITEVTAPLVFKD
                     VNSTYQALASVYSRLVGDAVYGIRVSLYYPLDNDEMQGPTGSSDNDRRDIARYAATPG
                     NAQLTNPFNQLFQGIEFANICIANIPQMDIYTNGTEQEKKKLQRMYGEALTLRAQFYF
                     EAIRNWGDLPAHFLPAATQSGSDPFPARVSMDTLYNRLIADLDMAEGLVPWRNEITGI
                     GDPLDERITKGTVKGLRARLALFRGGYALRSNGTMQRNADYQSFYQIARAECNEIMTS
                     GQHSLNPSFRGLWKDQVCARTLADPNGELMFQASATGGNATADTKLGYYNGPRVNNLG
                     NSSVNPLPTYFYLFDSTDARRDVTVCHYFTSSTGTRIGQPISNMTDGKYRRNWITNPV
                     VLPTNAIQYFSLKWQILRYADVLLMFAEAENELNGPTTAAYNAINMVRRRGFDKPINT
                     PDASVDIPAGLSKADFFKYIVRERALELGGEGIRKYDLIRWNLLGTALAESKANMLKM
                     AAATPMVDPSYMAGYPSYSLTNTLPA"
BASE COUNT          450 a          402 c          416 g          481 t
ORIGIN      
        1 ggcgggcagt gtattggtca gactatatga agggtagcct gccatataag atggatcaac
       61 cattggagta gctgcggcca ttttaagcat attagctttt gattcagcta aagctgtgcc
      121 taacagattc cagcggatca gatcatattt ccgaatacct tcaccaccta actcaagcgc
      181 tctttcccgt acgatatact taaagaaatc agccttggat aaaccggcag gaatatccac
      241 actggcatcc ggggtattga tcggtttgtc aaatcctctt ctgcgtacca tattaatcgc
      301 attataggca gcagttgtag ggccattaag ttcattttct gcttctgcaa acatcaataa
      361 cacatcggca taccggagaa tctgccattt aaggctaaaa tactgaatgg cattcgtggg
      421 taaaacaact ggattagtga tccagttacg gcggtattta ccatctgtca tatttgaaat
      481 aggttgtccg atacgtgttc cggtactact ggtaaaataa tgacaaaccg ttacatcacg
      541 ccgggcatca gtagaatcaa acagatagaa atatgtgggt agcgggttca cactgctgtt
      601 acccaggtta ttcacccgcg gaccattata atagccaagt tttgtatcgg ctgtggcatt
      661 accacctgtt gcactggcct ggaacatcaa ttcaccattc ggatcggcta atgttcgggc
      721 acaaacctga tccttccaga gtcctctgaa gcttggattc agcgagtgtt ggccggatgt
      781 catgatttcg ttgcattcgg ctctggcaat ctggtaaaag gattgatagt ctgcattacg
      841 ttgcatggta ccgttactgc ggagtgcata accgccacgg aataaggcca ggcgggcacg
      901 taatcctttt acagtgcctt tggtaatccg ttcatccagc ggatcgccga tgccggtgat
      961 ttcattccgc cagggcacca ggccttctgc catatccagg tcagcaatga gccggttata
     1021 taaagtgtcc atgctgactc tcgccgggaa ggggtcgctt ccggattggg tggcagcagg
     1081 caggaaatgt gccgggagat ccccccagtt gcggatggct tcaaaataaa actgggcacg
     1141 aagcgtaagt gcttcaccgt acatccgttg cagttttttc ttttcctgtt cggtaccatt
     1201 ggtatatata tccatctggg gaatattggc gatacagata ttcgcaaatt caatcccctg
     1261 gaaaagctgg ttaaacggat tcgtcagctg ggcatttccc ggtgtggcgg catagcgggc
     1321 aatatctctc cggtcattgt cagaggagcc cgttggtccc tgcatttcat cattatccag
     1381 tggataataa agactcacgc gtattccata aacggcatca cccacaagac gactatatac
     1441 actcgccagc gcctggtaag tagagtttac atctttaaat accaatgggg cggttacctc
     1501 agtaatgggt ttctggtcca ggaatttttt acaactgccg gccagcaggg ctatgataaa
     1561 ggcccccagt aaaacgattt tattataatt agtttttttc attttgaata agttgttgag
     1621 ttattaaaat gaagcattta caccaaagac aaaactgcgg cttttcggat acgcggagta
     1681 atcgaggccg ggtgtcagcg gattgcttct tacgctgact tccggatcat aacccgtgta
     1741 gttagtcag
//
LOCUS       DAOPMW010000459         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_3616
            NODE_91513_length_1714_cov_6.807715, whole genome shotgun sequence.
ACCESSION   DAOPMW010000459 DAOPMW010000000
VERSION     DAOPMW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865587
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:58:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,985
            CDSs (with protein)               :: 2,985
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 3 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91513_length_1714_cov_6.807715"
                     /isolate="AMR_MDS_3616"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLU11_14995"
     CDS             <1..471
                     /locus_tag="PLU11_14995"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPN60493.1"
                     /translation="NIPLIADGGIRYTGDMVKALAAGANCVMMGSIFAGTEESPGETI
                     IYEGRKFKEYRGMGSLGAMATGSSDRYFQDPEADIKKYVPEGIEGRVAYKGLLREVVY
                     QYTGGLRAGMGYCGAKNINDLQGATFVKITNAGMHESHAHDIDITKEAPNYSRR"
     gene            complement(584..1315)
                     /locus_tag="PLU11_15000"
     CDS             complement(584..1315)
                     /locus_tag="PLU11_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584500.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPN60494.1"
                     /translation="MKSYVTPLIIALSIITGVFVLGNAYKYKFKTQETISVTGLAETN
                     FVSDQIVWTGNYSRKTMDLKSAYSQLKEDESKIRSYLKGKGVTDAEMVFSAISIDKEF
                     SSRYDENGRITGSEFTGYNLKQNVTVDSKDIEKVEKISREVTELIESGIEFNSSSPSY
                     YYTKLSELKIDLLAKASADAKQRAETIAKNSGSGLGAIKKANMGVFQITGQNSDEDYS
                     YGGAFNTSSKNKTASITIKIEYAVD"
     gene            complement(1484..>1714)
                     /locus_tag="PLU11_15005"
     CDS             complement(1484..>1714)
                     /locus_tag="PLU11_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPN60495.1"
                     /translation="NNGAAPGRYSVEVKFVVDVEGHVSDIVALTKHGYGMEDEAIRVI
                     RKSAKWEPAVFEGRMVKAYRRQMITFVVEGEE"
BASE COUNT          451 a          427 c          368 g          468 t
ORIGIN      
        1 aatattccac tgatcgccga tggcggtatc cgttataccg gtgatatggt gaaagccctg
       61 gccgccggcg ccaactgcgt tatgatgggc agcatctttg ccggtacaga agaaagtccg
      121 ggtgaaacca tcatctacga aggacgtaaa ttcaaagaat accgaggtat gggttcactc
      181 ggggccatgg ccacgggcag cagtgaccgc tatttccagg atcccgaagc ggatataaag
      241 aaatatgtac ctgaaggaat cgaaggccgg gtggcttaca aaggattatt acgggaagta
      301 gtgtatcagt acacgggtgg actacgtgcc ggaatgggtt attgtggagc taaaaacatc
      361 aatgacctgc agggcgccac ctttgtcaaa atcaccaatg ccggtatgca tgaaagtcat
      421 gcacacgaca ttgatatcac caaagaagcg cctaactaca gtcgtcgctg acattgaaac
      481 aacaataaaa aaaagggtcc gatataacgg cccctttttt tataaaagct tgcattaaat
      541 actgaacttc cgctaaagaa caaacgtcta aacttctaaa caattagtca acggcatact
      601 cgatcttaat cgtaatcgac gccgtcttat tttttgagct ggtattgaag gctcctccgt
      661 aactataatc ctcatcactg ttctgtcccg ttatctggaa cacgcccata ttggctttct
      721 tgatcgctcc taatccgcta ccggaatttt tggcaatggt ctccgcccgt tgtttggcat
      781 ccgcactggc tttggccagc aggtcgatct tcagttcgga taatttggta tagtaatacg
      841 agggggagga agaattaaat tcaatcccgc tctcgatcag ttcggttact tcgcgggaga
      901 tcttttccac tttctcaata tcctttgaat ccactgttac attctgtttc aggttgtatc
      961 cggtaaattc actgccggta atccggccat tttcatcata gcggctggaa aactctttat
     1021 caattgaaat ggcagagaac accatttctg catcggtcac gcccttccct ttcaggtaag
     1081 acctgatctt gctttcatct tccttcagtt gtgaataagc ggatttaaga tccatcgtct
     1141 tgcggctgta attgccggtc cagacgatct gatcgctgac aaaattggtt tccgccagtc
     1201 cggtcacgct gatggtttcc tgggttttga atttgtactt gtaggcattg cctaaaacaa
     1261 aaacacccgt gataatcgac aacgcgatga tcagcggggt aacataactt ttcatgatgt
     1321 ggggttaatg gtaaaaataa ctgaacctaa agataacagg cctttcctat atttttcgtt
     1381 aattccagct gatatgacga aaatcactgt ccgcaccagc cagatactcc agggccggtg
     1441 cagacagcga ctactaactt ctaactgcta actacgaact ggttcattct tctccttcca
     1501 ccacaaacgt aatcatctgt ctccgatacg ctttcaccat ccggccttca aatacagccg
     1561 gttcccattt ggcggatttg cggatcacgc ggatcgcttc atcttccata ccatagccat
     1621 gtttggtcaa tgccacaata tcactgacat gcccttccac atccactacg aatttcactt
     1681 ctactgaata ccggcccgga gcggccccgt tgtt
//
